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Sample records for high antioxidant activity

  1. Highly antioxidant carotene-lipid nanocarriers: synthesis and antibacterial activity

    Energy Technology Data Exchange (ETDEWEB)

    Lacatusu, Ioana; Badea, Nicoleta, E-mail: nicoleta.badea@gmail.com; Ovidiu, Oprea [University POLITEHNICA of Bucharest, Faculty of Applied Chemistry and Materials Science (Romania); Bojin, Dionezie [Faculty of Engineering and Materials Science (Romania); Meghea, Aurelia [University POLITEHNICA of Bucharest, Faculty of Applied Chemistry and Materials Science (Romania)

    2012-06-15

    The objective of this study was to explore the potential of two natural oils (squalene-Sq and grape seed oil-GSO) to prepare biocompatible antioxidant nanostructured lipid carriers-NLCs as a safety and protective formulation for sensitive {beta}-carotene. For this purpose different oil-in-water nanoemulsions stabilized by a combination of alkylpolyoxy ethylene sorbitans, lecithin and a block copolymer, were prepared using a melt high-shear homogenization process. The physico-chemical characteristics of the carotene-loaded NLCs were firstly investigated in detail. The smaller lipid nanoparticles have been obtained by using Tween 20 as main non-ionic surfactant, with average diameters of about 85 nm for GSO and 89 nm for Sq, with a polydispersity index <0.19. The developed carotene-NLCs presented an excellent physical stability with almost all zeta potential values ranging between -29 Division-Sign -40 mV. The differential scanning calorimetry analysis showed that the {beta}-carotene incorporation has led to a perturbation of solid lipid matrix with a less ordered arrangement. By UV-Vis spectroscopy it was evidenced that after encapsulation {beta}-carotene adopts a supramolecular structure demonstrated by appearance of a shoulder at 530 nm related to a {beta}-carotene triplet-triplet absorption. The carotene-NLCs have been also evaluated in terms of in vitro antioxidant properties. The presence of Sq and GSO produced a significant effect on the antioxidant capacity of developed NLCs. The samples prepared with GSO and Tween 80 as main surfactant showed the highest antioxidant activity (AA %) against free oxygen radicals, exhibiting an enhancement of 35 % for loaded NLCs, as comparing to pure carotene. In addition to these properties, the ability of NLCs to manifest antibacterial activity was tested against Escherichiacoli bacteria. The antibacterial analysis shown that loaded-NLCs develop an effective inhibition zone against bacteria growth and it was dependent in a

  2. Tetrahydrofolate and 5-methyltetrahydrofolate are folates with high antioxidant activity. Identification of the antioxidant pharmacophore.

    NARCIS (Netherlands)

    Rezk, BM; Haenen, GR; Vijgh, van der W.J.F.

    2003-01-01

    The presumed protective effect of folic acid on the pathogenesis of cardiovascular, hematological and neurological diseases and cancer has been associated with the antioxidant activity of folic acid. Peroxynitrite (PON) scavenging activity and inhibition of lipid peroxidation (LPO) of the physiologi

  3. Effects of Glycyrrhiza glabra polysaccharides on immune and antioxidant activities in high-fat mice.

    Science.gov (United States)

    Hong, Ying-Kai; Wu, Hua-Tao; Ma, Tao; Liu, Wei-Juan; He, Xue-Jun

    2009-07-01

    The purpose of this study was to investigate the immune and antioxidant activities of Glycyrrhiza glabra polysaccharides (GGP) in rats fed high-fat diet. The experiment was performed on four groups of growing Kunming mice. The results of the experiment showed a statistically significant decrease in serum antioxidant enzyme activities in high-fat group. Administration of GGP dose-dependently significantly enhanced immune and antioxidant enzyme activities in the GGP-treated mice compared to the high-fat model mice. It is concluded that GGP treatment can enhance immune activities, and reduce oxidative stress in high-fat mice.

  4. Enhanced antioxidant and antityrosinase activities of longan fruit pericarp by ultra-high-pressure-assisted extraction.

    Science.gov (United States)

    Prasad, K Nagendra; Yang, Bao; Shi, John; Yu, Chunyan; Zhao, Mouming; Xue, Sophia; Jiang, Yueming

    2010-01-20

    The health benefits of fruits acting against chronic diseases are ascribed to their antioxidant activities which are mainly responsible due to the presence of phenolic compounds. The use of ultra-high-pressure-assisted extraction (UHPE) has shown great advantages for the extraction of these phenolic compounds from longan fruit pericarp (LFP). Studies were carried out to investigate the effects of UHPE at pressures of 200, 300, 400 and 500 MPa on total phenolic contents, extraction yield, antioxidant and antityrosinase activities from LFP. The antioxidant activities of these extracts were analyzed, using various antioxidant models like 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, total antioxidant capacity and superoxide anion radical scavenging activity. Extract from ultra-high-pressure-assisted extraction at 500MPa (UHPE-500) showed the highest antioxidant activities of all the tested models. In addition, it also showed moderate tyrosinase inhibitory activity. Three phenolic acids, namely gallic acid, ellagic acid, and corilagin were identified and quantified by HPLC. Corilagin content was the highest compared to other phenolic acids identified. UHPE-500 obtained the higher phenolic acid contents compared to other high pressure processing and conventional extractions (CE). Compared with CE, UHPE-500 exhibited good extraction effectiveness in terms of higher extraction yields with high phenolic contents and also with higher antioxidant and antityrosinase activities.

  5. Highly antioxidant carotene-lipid nanocarriers: synthesis and antibacterial activity

    Science.gov (United States)

    Lacatusu, Ioana; Badea, Nicoleta; Ovidiu, Oprea; Bojin, Dionezie; Meghea, Aurelia

    2012-06-01

    The objective of this study was to explore the potential of two natural oils (squalene—Sq and grape seed oil—GSO) to prepare biocompatible antioxidant nanostructured lipid carriers—NLCs as a safety and protective formulation for sensitive β-carotene. For this purpose different oil-in-water nanoemulsions stabilized by a combination of alkylpolyoxy ethylene sorbitans, lecithin and a block copolymer, were prepared using a melt high-shear homogenization process. The physico-chemical characteristics of the carotene-loaded NLCs were firstly investigated in detail. The smaller lipid nanoparticles have been obtained by using Tween 20 as main non-ionic surfactant, with average diameters of about 85 nm for GSO and 89 nm for Sq, with a polydispersity index Escherichia coli bacteria. The antibacterial analysis shown that loaded-NLCs develop an effective inhibition zone against bacteria growth and it was dependent in a higher extent on the liquid lipid and carotene concentrations than on their particle size.

  6. Investigation of the antioxidant activity of chitooligosaccharides on mice with high-fat diet

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    Daofeng Qu

    Full Text Available ABSTRACT The objective of this study was to analyze the antioxidant activities of chitooligosaccharides (COS both in vitro and in high-fat diet (HFD-mouse model. In antioxidant assays in HFD-mouse model, mice were administered with normal diet, HFD, or HFD with 0.5% COS for six weeks. The administration of HFD with 0.5% COS resulted in significant increase in the activity of superoxide dismutase, catalase, and glutathione peroxidase in stomach, liver, and serum of mice as compared with the HFD-fed group, which means that COS may have certain antioxidant activity and can restore the activity of the enzymes affected by the HFD. Through morphological measurements of the small intestinal mucosa, mice fed HFD showed decreased villus height compared with other groups. On the other hand, HFD with 0.5% COS group showed similar ratio of villus height to depth compared with control mice, indicating that intestinal integrity was improved when COS was added. Chitooligosaccharides have potent antioxidant activity that can protect mice from oxidative stress.

  7. High Performance Liquid Chromatography-mass Spectrometry Analysis of High Antioxidant Australian Fruits with Antiproliferative Activity Against Cancer Cells

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    Sirdaarta, Joseph; Maen, Anton; Rayan, Paran; Matthews, Ben; Cock, Ian Edwin

    2016-01-01

    Background: High antioxidant capacities have been linked to the treatment and prevention of several cancers. Recent reports have identified several native Australian fruits with high antioxidant capacities. Despite this, several of these species are yet to be tested for anticancer activity. Materials and Methods: Solvent extracts prepared from high antioxidant native Australian fruits were analyzed for antioxidant capacity by the di (phenyl)-(2,4,6-trinitrophenyl) iminoazanium free radical scavenging assay. Antiproliferative activities against CaCo2 and HeLa cancer cells were determined by a multicellular tumor spheroid-based cell proliferation assay. Toxicity was determined by Artemia franciscana bioassay. Results: Methanolic extracts of all plant species displayed high antioxidant contents (equivalent to approximately 7–16 mg of vitamin C per gram of fruit extracted). Most aqueous extracts also contained relatively high antioxidant capacities. In contrast, the ethyl acetate, chloroform, and hexane extracts of most species (except lemon aspen and bush tomato) had lower antioxidant contents (below 1.5 mg of vitamin C equivalents per gram of plant material extracted). The antioxidant contents correlated with the ability of the extracts to inhibit proliferation of CaCo2 and HeLa cancer cell lines. The high antioxidant methanolic extracts of all species were potent inhibitors of cell proliferation. The methanolic lemon aspen extract was particularly effective, with IC50 values of 480 and 769 μg/mL against HeLa and CaCo2 cells, respectively. In contrast, the lower antioxidant ethyl acetate and hexane extracts (except the lemon aspen ethyl acetate extract) generally did not inhibit cancer cell proliferation or inhibited to only a minor degree. Indeed, most of the ethyl acetate and hexane extracts induced potent cell proliferation. The native tamarind ethyl acetate extract displayed low-moderate toxicity in the A. franciscana bioassay (LC50 values below 1000

  8. Fingerprinting antioxidative activities in plants

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    Saleh, Livia; Plieth, Christoph

    2009-01-01

    Background A plethora of concurrent cellular activities is mobilised in the adaptation of plants to adverse environmental conditions. This response can be quantified by physiological experiments or metabolic profiling. The intention of this work is to reduce the number of metabolic processes studied to a minimum of relevant parameters with a maximum yield of information. Therefore, we inspected 'summary parameters' characteristic for whole classes of antioxidative metabolites and key enzymes. Results Three bioluminescence assays are presented. A horseradish peroxidase-based total antioxidative capacity (TAC) assay is used to probe low molecular weight antioxidants. Peroxidases are quantified by their luminol converting activity (LUPO). Finally, we quantify high molecular weight superoxide anion scavenging activity (SOSA) using coelenterazine. Experiments with Lepidium sativum L. show how salt, drought, cold, and heat influence the antioxidative system represented here by TAC, LUPO, SOSA, catalase, and glutathione reductase (GR). LUPO and SOSA run anti-parallel under all investigated stress conditions suggesting shifts in antioxidative functions rather than formation of antioxidative power. TAC runs in parallel with GR. This indicates that a majority of low molecular weight antioxidants in plants is represented by glutathione. Conclusion The set of assays presented here is capable of characterising antioxidative activities in plants. It is inexpensive, quick and reproducible and delivers quantitative data. 'Summary parameters' like TAC, LUPO, and SOSA are quantitative traits which may be promising for implementation in high-throughput screening for robustness of novel mutants, transgenics, or breeds. PMID:19171044

  9. High Antioxidant Activity Facilitates Maintenance of Cell Division in Leaves of Drought Tolerant Maize Hybrids

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    Avramova, Viktoriya; AbdElgawad, Hamada; Vasileva, Ivanina; Petrova, Alexandra S.; Holek, Anna; Mariën, Joachim; Asard, Han; Beemster, Gerrit T. S.

    2017-01-01

    We studied the impact of drought on growth regulation in leaves of 13 maize varieties with different drought sensitivity and geographic origins (Western Europe, Egypt, South Africa) and the inbred line B73. Combining kinematic analysis of the maize leaf growth zone with biochemical measurements at a high spatial resolution allowed us to examine the correlation between the regulation of the cellular processes cell division and elongation, and the molecular redox-regulation in response to drought. Moreover, we demonstrated differences in the response of the maize lines to mild and severe levels of water deficit. Kinematic analysis indicated that drought tolerant lines experienced less impact on leaf elongation rate due to a smaller reduction of cell production, which, in turn, was due to a smaller decrease of meristem size and number of cells in the leaf meristem. Clear differences in growth responses between the groups of lines with different geographic origin were observed in response to drought. The difference in drought tolerance between the Egyptian hybrids was significantly larger than between the European and South-African hybrids. Through biochemical analyses, we investigated whether antioxidant activity in the growth zone, contributes to the drought sensitivity differences. We used a hierarchical clustering to visualize the patterns of lipid peroxidation, H2O2 and antioxidant concentrations, and enzyme activities throughout the growth zone, in response to stress. The results showed that the lines with different geographic region used different molecular strategies to cope with the stress, with the Egyptian hybrids responding more at the metabolite level and African and the European hybrids at the enzyme level. However, drought tolerance correlated with both, higher antioxidant levels throughout the growth zone and higher activities of the redox-regulating enzymes CAT, POX, APX, and GR specifically in leaf meristems. These findings provide evidence for a link

  10. Salicylic acid promotes seed germination under high salinity by modulating antioxidant activity in Arabidopsis.

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    Lee, Sangmin; Kim, Sang-Gyu; Park, Chung-Mo

    2010-10-01

    • Findings regarding the role of salicylic acid (SA) in seed germination are somewhat variable, depending on the plant genotypes and experimental conditions used, and thus the molecular mechanisms underlying SA regulation of germination are still unclear. Here, we report that physiological concentrations of SA promote germination under high salinity by modulating antioxidant activity in Arabidopsis. • Germination of SA induction deficient 2 (sid2) seeds was hypersensitive to high salinity. While the inhibitory effect of high salinity was exaggerated in the presence of higher concentrations of SA (> 100 μM), it was significantly reduced in the presence of lower concentrations of SA (salinity, the endogenous contents of H(2) O(2) were elevated in wild-type and sid2 seeds but reduced to original concentrations after treatment with 1 μM SA. • Germination of NahG transgenic plants was influenced to a lesser degree by high salinity (NahG is a bacterial gene encoding salicylate hydroxylase that converts salicylic acid to catechol). We found that catechol, an SA degradation product accumulated in the transgenic plants, acts as an antioxidant that compromises the inhibitory effects of high salinity. • Our observations indicate that, although SA is not essential for germination under normal growth conditions, it plays a promotive role in seed germination under high salinity by reducing oxidative damage.

  11. Assessment of antioxidant activity in Victorian marine algal extracts using high performance thin-layer chromatography and multivariate analysis.

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    Agatonovic-Kustrin, Snezana; Morton, David W; Ristivojević, Petar

    2016-10-14

    The aim of this study was to develop and validate a rapid and simple high performance thin layer chromatographic (HPTLC) method to screen for antioxidant activity in algal samples. 16 algal species were collected from local Victorian beaches. Fucoxanthin, one of the most abundant marine carotenoids was quantified directly from the HPTLC plates before derivatization, while derivatization either with 2,2-diphenyl-1-picrylhydrazyl (DPPH) or ferric chloride (FeCl3) was used to analyze antioxidants in marine algae, based on their ability to scavenge non biological stable free radical (DPPH) or to chelate iron ions. Principal component analysis of obtained HPTLC fingerprints has classified algae species into 5 groups according to their chemical/antioxidant profiles. The investigated brown algae samples were found to be rich in non-and moderate-polar compounds and phenolic compounds with antioxidant activity. Most of the phenolic iron chelators also have shown free radical scavenging activity. Strong positive and significant correlations between total phenolic content and DPPH radical scavenging activity showed that, phenolic compounds, including flavonoids are the main contributors of antioxidant activity in these species. The results suggest that certain brown algae possess significantly higher antioxidant potential when compared to red or green algae and could be considered for future applications in medicine, dietary supplements, cosmetics or food industries. Cystophora monilifera extract was found to have the highest antioxidant concentration, followed by Zonaria angustata, Cystophora pectinate, Codium fragile, and Cystophora pectinata. Fucoxanthin was found mainly in the brown algae species. The proposed methods provide an edge in terms of screening for antioxidants and quantification of antioxidant constituents in complex mixtures. The current application also demonstrates flexibility and versatility of a standard HPTLC system in the drug discovery. Proposed

  12. Methods for testing antioxidant activity.

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    Antolovich, Michael; Prenzler, Paul D; Patsalides, Emilios; McDonald, Suzanne; Robards, Kevin

    2002-01-01

    Antioxidant activity has been assessed in many ways. The limitation of many newer methods is the frequent lack of an actual substrate in the procedure. The combination of all approaches with the many test methods available explains the large variety of ways in which results of antioxidant testing are reported. The measurement of antioxidant activities, especially of antioxidants that are mixtures, multifunctional or are acting in complex multiphase systems, cannot be evaluated satisfactorily by a simple antioxidant test without due regard to the many variables influencing the results. Several test procedures may be required to evaluate such antioxidant activities. A general method of reporting antioxidant activity independent of the test procedure is proposed.

  13. Membrane processes application on the Symphytum officinale and Geranium robertianum extracts concentration to obtain high antioxidative activity compounds

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    Paun Gabriela

    2012-01-01

    Full Text Available The paper reports the successful application of membranes processes to obtain the good quality extracts with compounds of high antioxidative activity and therapeutic value. This study provided investigations on the phenolic compounds from two plant species used in Romanian ethno-medicine and evaluated the antioxidant and cytotoxic activities. Three extracts prepared from Geranium robertianum and Symphytum officinale were concentrated by microfiltration and ultrafiltration. The levels of phenolic compounds and flavonoids were determined by UV-Vis spectroscopy and HPLC. The freeradical scavenging activity of concentrated extracts was determined by DPPH method. The preliminary tests of cytotoxic activity for the concentrated extracts have been carried out on human epidermoid laryngeal carcinoma cell line (Hep-2p and normal monkey kidneys cells (RM. The results showed that all concentrated extracts had a very low cytotoxicity against healthy cells, but a significant cytotoxic effect on Hep-2p tumor cells. The concentrated extracts have a high antioxidant activity (% DPPH inhibition > 80%.

  14. N-Nicotinoyl dopamine, a novel niacinamide derivative, retains high antioxidant activity and inhibits skin pigmentation.

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    Kim, Bora; Kim, Jin Eun; Lee, Su Min; Lee, Soung-Hoon; Lee, Jin Won; Kim, Myung Kyoo; Lee, Kye Jong; Kim, Hyuk; Lee, Joo Dong; Choi, Kang-Yell

    2011-11-01

    We synthesized a novel derivative of a well-known skin-lightening compound niacinamide, N-nicotinoyl dopamine (NND). NND did not show inhibitory effects of tyrosinase and melanin synthesis in B16F10 mouse melanoma cells. However, NND retains high antioxidant activity without affecting viability of cells. In a reconstructed skin model, topical applications of 0.05% and 0.1% NND induced skin lightening and decreased melanin production without affecting the viability and morphology of melanocytes and overall tissue histology. Moreover, no evidence for skin irritation or sensitization was observed when 0.1% NND emulsion was applied onto the skin of 52 volunteers. The effect of NND on skin lightening was further revealed by pigmented spot analyses of human clinical trial. Overall, NND treatment may be a useful trial for skin lightening and treating pigmentary disorders.

  15. Encapsulation of phycocyanin-alginate for high stability and antioxidant activity

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    Hadiyanto; Suzery, Meiny; Setyawan, Deny; Majid, Dian; Sutanto, Heri

    2017-02-01

    The aim of this study was to obtain optimal condition of phycocyanin-alginate encapsulation, encapsulation efficiency and phycocyanin load, physicochemical properties of beads, in vitro release study, stability and antioxidant activity. The result product with alginate content 1,5% (w/v) and 2% (w/v) produced were in spherical shape than product with alginate content 2,5% (w/v) by ratio of phycocyanin 1:1. Increasing alginate content on encapsulation process will increase of encapsulation efficiency and phycocyanin load. In vitro released study showed that phycocyanin-alginate beads were more resistant in simulated gastric fluid, while rapidly release in simulated intestinal fluid. The antioxidant activity showed that phycocyanin antioxidant activity decreased after encapsulation process due to duration of storage and the possibility of a cracking which will cause reduced stability of phycocyanin.

  16. Production of monodisperse epigallocatechin gallate (EGCG) microparticles by spray drying for high antioxidant activity retention.

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    Fu, Nan; Zhou, Zihao; Jones, Tyson Byrne; Tan, Timothy T Y; Wu, Winston Duo; Lin, Sean Xuqi; Chen, Xiao Dong; Chan, Peggy P Y

    2011-07-15

    Epigallocatechin gallate (EGCG) originated from green tea is well-known for its pharmaceutical potential and antiproliferating effect on carcinoma cells. For drug delivery, EGCG in a micro-/nanoparticle form is desirable for their optimized chemopreventive effect. In this study, first time reports that EGCG microparticles produced by low temperature spray drying can maintain high antioxidant activity. A monodisperse droplet generation system was used to realize the production of EGCG microparticles. EGCG microparticles were obtained with narrow size distribution and diameter of 30.24 ± 1.88 μM and 43.39 ± 0.69 μM for pure EGCG and lactose-added EGCG, respectively. The EC50 value (the amount of EGCG necessary to scavenge 50% of free radical in the medium) of spray dried pure EGCG particles obtained from different temperature is in the range of 3.029-3.075 μM compared to untreated EGCG with EC50 value of 3.028 μM. Varying the drying temperatures from 70°C and 130°C showed little detrimental effect on EGCG antioxidant activity. NMR spectrum demonstrated the EGCG did not undergo chemical structural change after spray drying. The major protective mechanism was considered to be: (1) the use of low temperature and (2) the heat loss from water evaporation that kept the particle temperature at low level. With further drier optimization, this monodisperse spray drying technique can be used as an efficient and economic approach to produce EGCG micro-/nanoparticles.

  17. High value co-products from wine byproducts (II): polyphenols and antioxidant activity

    Energy Technology Data Exchange (ETDEWEB)

    Femenia, A.; Gonzalez-Centeno, M. R.; Garau, M. C.; Sastre-Serrano, G.; Rosello, C.

    2009-07-01

    The by-products of the grape/wine industry have recently attracted considerable interest as important sources of high-value antioxidants. these can be extracted from stems, such as resveratrol,and from grape pomace which contains polyphenols, procyanidin and antrocyanins. (Author)

  18. Antioxidative activity of high-density lipoprotein (HDL): Mechanistic insights into potential clinical benefit.

    Science.gov (United States)

    Brites, Fernando; Martin, Maximiliano; Guillas, Isabelle; Kontush, Anatol

    2017-12-01

    Uptake of low-density lipoprotein (LDL) particles by macrophages represents a key step in the development of atherosclerotic plaques, leading to the foam cell formation. Chemical modification of LDL is however necessary to induce this process. Proatherogenic LDL modifications include aggregation, enzymatic digestion and oxidation. LDL oxidation by one-electron (free radicals) and two-electron oxidants dramatically increases LDL affinity to macrophage scavenger receptors, leading to rapid LDL uptake and fatty streak formation. Circulating high-density lipoprotein (HDL) particles, primarily small, dense, protein-rich HDL3, provide potent protection of LDL from oxidative damage by free radicals, resulting in the inhibition of the generation of pro-inflammatory oxidized lipids. HDL-mediated inactivation of lipid hydroperoxides involves their initial transfer from LDL to HDL and subsequent reduction to inactive hydroxides by redox-active Met residues of apolipoprotein A-I. Several HDL-associated enzymes are present at elevated concentrations in HDL3 relative to large, light HDL2 and can be involved in the inactivation of short-chain oxidized phospholipids. Therefore, HDL represents a multimolecular complex capable of acquiring and inactivating proatherogenic lipids. Antioxidative function of HDL can be impaired in several metabolic and inflammatory diseases. Structural and compositional anomalies in the HDL proteome and lipidome underlie such functional deficiency. Concomitant normalization of the metabolism, circulating levels, composition and biological activities of HDL particles, primarily those of small, dense HDL3, can constitute future therapeutic target.

  19. Antioxidant Activity from Various Tomato Processing

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    Retno Sri Iswari

    2016-04-01

    Full Text Available Tomato is one of the high antioxidant potential vegetables. Nowadays, there are many techniques of tomato processings instead of fresh consumption, i.e. boiled, steamed, juiced and sauteed. Every treatment of cooking will influence the chemical compound inside the fruits and the body's nutrition intake. It is important to conduct the research on antioxidant compound especially lycopene, β-carotene, vitamin C, α-tocopherol, and its activity after processing. This research has been done using the experimental method. Tomatoes were cooked into six difference ways, and then it was extracted using the same procedure continued with antioxidant measurement. The research results showed that steaming had promoted the higher antioxidant numbers (lycopene. α-tocopherol, β-carotene and vitamin C and higher TCA and antioxidant activities in the tomatoes than other processings. It was indicated that steaming was the best way to enhance amount, capacity and activities of antioxidants of the tomatoes.

  20. Antioxidant activity of Citrus fruits.

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    Zou, Zhuo; Xi, Wanpeng; Hu, Yan; Nie, Chao; Zhou, Zhiqin

    2016-04-01

    Citrus is well-known for its nutrition and health-promotion values. This reputation is derived from the studies on the biological functions of phytochemicals in Citrus fruits and their derived products in the past decades. In recent years, the antioxidant activity of Citrus fruits and their roles in the prevention and treatment of various human chronic and degenerative diseases have attracted more and more attention. Citrus fruits are suggested to be a good source of dietary antioxidants. To have a better understanding of the mechanism underlying the antioxidant activity of Citrus fruits, we reviewed a study on the antioxidant activity of the phytochemicals in Citrus fruits, introduced methods for antioxidant activity evaluation, discussed the factors which influence the antioxidant activity of Citrus fruits, and summarized the underlying mechanism of action. Some suggestions for future study were also presented.

  1. Coffee modulates transcription factor Nrf2 and highly increases the activity of antioxidant enzymes in rats.

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    Vicente, Silvio J V; Ishimoto, Emília Y; Torres, Elizabeth A F S

    2014-01-08

    This study investigated the effect of a 28 day administration of coffee brew on the activity of antioxidant enzymes in rats. After this period of 2.0 mL/day dosages of this beverage, the activities of hepatic superoxide dismutase, catalase, and glutathione peroxidase increased 74.8, 59.4, and 135.2%, respectively, whereas the cytosolic level of Nrf2 increased 131.3%. At the same time, the total antioxidant capacity of the hepatic tissue increased 25.1%, improving the defensive status against oxidative stress. At the end of the experiment, the levels of biomarkers alanine transaminase and aspartate transaminase remained equal to the control group, and no changes were observed in the hepatic histoarchiteture of the animals, suggesting that the liver tissue was not impaired by the exposure to coffee. The changes in enzyme activities and antioxidant capacity were statistically significant (p < 0.05), indicating that coffee could be considered an important alternative against oxidative stress and its correlated degenerative diseases.

  2. Investigation of antioxidant, antimicrobial and toxicity activities of lichens from high altitude regions of Nepal.

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    Jha, Baidya Nath; Shrestha, Mitesh; Pandey, Durga Prasad; Bhattarai, Tribikram; Bhattarai, Hari Datta; Paudel, Babita

    2017-05-25

    Several lichen species are reported to be used tradiationally in many theraupatic practices. Many lichen species are reported as sources of several bioactive natural compounds. Several lichen species of Nepal are so far chemically unexplored. The morphological, anatomical and phytochemical characteristics of lichens were compared for the taxonomic identification of the species. Methanol- water extract of lichens were sub fractionated into hexane, dichloromethane and methanol fractions for bioactivity assays. Antimicrobial activities of extracts were evaluated agaisnt pathogenic bacteria and fungal species. DPPH test was used for antioxidant potential evaluation. Brineshrimp test was perfermed to evaluate toxicity of the extracts. A total of 84 lichen specimens were collected and identified from Annapurna Conservation Area (ACA) Nepal. The specimens were identified as belonging to 19 genera and 47 species. Methanol fractions of 16 specimens and dichloromethane (DCM) fractions of 21 lichens specimens showed antioxidant activities comparable with commercial standards (BHA, Butylated hydroxyanisole, IC50=4.9±0.9 μg/mL) even at crude extract level. Similarly, the DCM fraction of 17 lichens showed potential antimicrobial activity against a Gram-positive bacterium (Staphylococcus aureus KCTC3881) and DCM fractions of 45 lichens showed antimicrobial activity against a Gram-negative bacterium (Klebsiella pneumoniae KCTC2242). DCM fractions of three lichens showed antifungal activity against the yeast, Candida albicans KCTC 7965. Likewise, methanol fractions of 39 lichens and DCM fractions of 74 lichens showed strong toxicity against brine shrimp nauplii with more than 80% mortality. Such biological activity-rich lichen specimens warrant further research on exploration of natural products with antioxidant, antimicrobial and anti cancer (toxic) potential.

  3. In search of novel highly active mitochondria-targeted antioxidants: thymoquinone and its cationic derivatives.

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    Severina, Inna I; Severin, Fedor F; Korshunova, Galina A; Sumbatyan, Natalya V; Ilyasova, Tatyana M; Simonyan, Ruben A; Rogov, Anton G; Trendeleva, Tatyana A; Zvyagilskaya, Renata A; Dugina, Vera B; Domnina, Lidia V; Fetisova, Elena K; Lyamzaev, Konstantin G; Vyssokikh, Mikhail Yu; Chernyak, Boris V; Skulachev, Maxim V; Skulachev, Vladimir P; Sadovnichii, Viktor A

    2013-06-27

    Since the times of the Bible, an extract of black cumin seeds was used as a medicine to treat many human pathologies. Thymoquinone (2-demethylplastoquinone derivative) was identified as an active antioxidant component of this extract. Recently, it was shown that conjugates of plastoquinone and penetrating cations are potent mitochondria-targeted antioxidants effective in treating a large number of age-related pathologies. This review summarizes new data on the antioxidant and some other properties of membrane-penetrating cationic compounds where 2-demethylplastoquinone substitutes for plastoquinone. It was found that such a substitution significantly increases a window between anti- and prooxidant concentrations of the conjugates. Like the original plastoquinone derivatives, the novel compounds are easily reduced by the respiratory chain, penetrate through model and natural membranes, specifically accumulate in mitochondria in an electrophoretic fashion, and strongly inhibit H2O2-induced apoptosis at pico- and nanomolar concentrations in cell cultures. At present, cationic demethylplastoquinone derivatives appear to be the most promising mitochondria-targeted drugs of the quinone series.

  4. High frequency in vitro plantlet regeneration and antioxidant activity of Enicostema axillare(Lam. Raynal ssp. littoralis (Blume Raynal: An important medicinal plant

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    Kousalya Loganathan

    2014-09-01

    Conclusion: The present study not only enables reinforcement of wild plant populations using ex situ growth of individuals, but it also helps for high scale production of plantlets. A high correlation between antioxidant capacities and their total phenolic contents indicated that flavonoid compounds were a major contributor of antioxidant activity of both plants grown from nature and nodal culture.

  5. A cytochrome c mutant with high electron transfer and antioxidant activities but devoid of apoptogenic effect.

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    Abdullaev, Ziedulla Kh; Bodrova, Marina E; Chernyak, Boris V; Dolgikh, Dmitry A; Kluck, Ruth M; Pereverzev, Mikhail O; Arseniev, Alexander S; Efremov, Roman G; Kirpichnikov, Mikhail P; Mokhova, Elena N; Newmeyer, Donald D; Roder, Heinrich; Skulachev, Vladimir P

    2002-01-01

    A cytochrome c mutant lacking apoptogenic function but competent in electron transfer and antioxidant activities has been constructed. To this end, mutant species of horse and yeast cytochromes c with substitutions in the N-terminal alpha-helix or position 72 were obtained. It was found that yeast cytochrome c was much less effective than the horse protein in activating respiration of rat liver mitoplasts deficient in endogenous cytochrome c as well as in inhibition of H(2)O(2) production by the initial segment of the respiratory chain of intact rat heart mitochondria. The major role in the difference between the horse and yeast proteins was shown to be played by the amino acid residue in position 4 (glutamate in horse, and lysine in yeast; horse protein numbering). A mutant of the yeast cytochrome c containing K4E and some other "horse" modifications in the N-terminal alpha-helix, proved to be (i) much more active in electron transfer and antioxidant activity than the wild-type yeast cytochrome c and (ii), like the yeast cytochrome c, inactive in caspase stimulation, even if added in 400-fold excess compared with the horse protein. Thus this mutant seems to be a good candidate for knock-in studies of the role of cytochrome c-mediated apoptosis, in contrast with the horse K72R, K72G, K72L and K72A mutant cytochromes that at low concentrations were less active in apoptosis than the wild-type, but were quite active when the concentrations were increased by a factor of 2-12. PMID:11879204

  6. Antioxidant activity of polyaniline nanofibers

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Well-confined uniform polyaniline (PANT) nanofibers were synthesized by using photo-assisted chemical oxidative polymerization of aniline in the presence of different dopant acids, and the radical scavenging ability of the produced PANI nanofibers was determined by the DPPH assay. It was found that the antioxidant activity of PANI nanofibers was higher than conventional PANI,and increased with decreasing of averaged diameter of the nanofibers. The enhanced antioxidant activity was concerned with increased surface area of PANI nanofibers.

  7. Determination of antioxidant activity of milk - vegetable extracts byliquid chromatography

    Directory of Open Access Journals (Sweden)

    E. S. Rudnichenko

    2012-01-01

    Full Text Available Antioxidant activity of milk-plant extract of natural sweetener yakon has been established. To extract yakon solid tubers for the first time as an extractant used ultrafiltrate of cheese whey - a product of the extraction separation of whey. Antioxidant activity of extracts was measured amperometrically yakon on the liquid chromatograph "Colour-Yauza-01-AA."A technique for measuring the content of antioxidant. The equations for calculating antioxidant activityyakon. The total content of natural antioxidants can be recommended extract yakon to create new foods with high antioxidant activity.

  8. Effects of high hydrostatic pressure and thermal processing on bioactive compounds, antioxidant activity, and volatile profile of mulberry juice.

    Science.gov (United States)

    Wang, Fan; Du, Bao-Lei; Cui, Zheng-Wei; Xu, Li-Ping; Li, Chun-Yang

    2017-03-01

    The aim of this study was to investigate the effects of high hydrostatic pressure and thermal processing on microbiological quality, bioactive compounds, antioxidant activity, and volatile profile of mulberry juice. High hydrostatic pressure processing at 500 MPa for 10 min reduced the total viable count from 4.38 log cfu/ml to nondetectable level and completely inactivated yeasts and molds in raw mulberry juice, ensuring the microbiological safety as thermal processing at 85 ℃ for 15 min. High hydrostatic pressure processing maintained significantly (p juice than thermal processing. The main volatile compounds of mulberry juice were aldehydes, alcohols, and ketones. High hydrostatic pressure processing enhanced the volatile compound concentrations of mulberry juice while thermal processing reduced them in comparison with the control. These results suggested that high hydrostatic pressure processing could be an alternative to conventional thermal processing for production of high-quality mulberry juice.

  9. Antiradical and antioxidant activities of new bio-antioxidants.

    Science.gov (United States)

    Kancheva, V D; Saso, L; Angelova, S E; Foti, M C; Slavova-Kasakova, A; Daquino, C; Enchev, V; Firuzi, O; Nechev, J

    2012-02-01

    Antioxidants could be promising agents for management of oxidative stress-related diseases. New biologically active compounds, belonging to a rare class of natural lignans with antiangiogenic, antitumoral and DNA intercalating properties, have been recently synthesized. These compounds are benzo[kl]xanthene lignans (1,2) and dihydrobenzofuran neolignans (3,4). The radical scavenging and chain-breaking antioxidant activities of compounds 1-4 were studied by applying different methods: radical scavenging activity by DPPH rapid test, chain-breaking antioxidant activity and quantum chemical calculations. All studied compounds were found to be active as DPPH scavengers but reaction time with DPPH and compounds' concentrations influenced deeply the evaluation. The highest values of radical scavenging activity (%RSAmax) and largest rate constants for reaction with DPPH were obtained for compounds 2 and 3. Comparison of %RSAmax with that of standard antioxidants DL-α-tocopherol (TOH), caffeic acid (CA) and butylated hydroxyl toluene (BHT) give the following new order of %RSA max: TOH (61.1%) > CA (58.6%) > 3 (36.3%) > 2 (28.1%) > 4 (6.7%) > 1 (3.6%) = BHT (3.6%). Chain-breaking antioxidant activities of individual compounds (0.1-1.0 mM) and of their equimolar binary mixtures (0.1 mM) with TOH were determined from the kinetic curves of lipid autoxidation at 80 °C. On the basis of a comparable kinetic analysis with standard antioxidants a new order of the antioxidant efficiency (i.e., protection factor, PF) of compounds 1-4 were obtained: 2 (7.2) ≥ TOH (7.0) ≥ CA (6.7) > 1 (3.1) > 3 (2.2) > ferulic acid FA (1.5) > 4 (0.6); and of the antioxidant reactivity (i.e. inhibition degree, ID): 2 (44.0) > TOH (18.7) > CA (9.3) > 1 (8.4) > 3 (2.8) > FA (1.0) > 4 (0.9). The important role of the catecholic structure in these compounds, which is responsible for the high chain-breaking antioxidant activity, is discussed and a reaction

  10. Effects of tryptophan supplementation on growth performance, antioxidative activity, and meat quality of ducks under high stocking density.

    Science.gov (United States)

    Liu, Y; Yuan, J M; Zhang, L S; Zhang, Y R; Cai, S M; Yu, J H; Xia, Z F

    2015-08-01

    High stocking density (STD) could affect duck welfare and production. The objective of our study was to investigate whether dietary tryptophan (TRP) supplementation could alleviate the detrimental effects of high STD on ducks. White Pekin ducks at 4 to 6 wk of age were raised at 11 birds/m(2) and fed diets containing 0.18, 0.48, 0.78, or 1.08% TRP for 21 d. Growth performance, concentrations of TRP and metabolites in the blood and hypothalamus, antioxidative activities in serum and tissue, meat quality, serum uric acid, and urea nitrogen were measured. Weight gain and feed efficiency were significantly improved by TRP supplementation at ≥ 0.48 and ≥ 0.78% (P 0.05). Breast muscle shear force was increased significantly when dietary TRP level increased to 1.08% (P ducks raised at 11 birds/m², dietary TRP supplementation could alleviate stress and improve growth performance, antioxidative activity, and meat quality.

  11. The effect of high-pressure processing on colour, bioactive compounds, and antioxidant activity in smoothies during refrigerated storage.

    Science.gov (United States)

    Andrés, Víctor; Villanueva, María J; Tenorio, María D

    2016-02-01

    The effects of high-pressure processing--HPP--(450 and 600 MPa/3 min/20 °C) on the colour, carotenoids, ascorbic acid, polyphenols and antioxidant activity (FRAP and DPPH) of a smoothie were compared to thermal processing (80 °C/3 min). Stability during 45 days at 4 °C was also evaluated. HPP samples showed slight differences (p colour compared to untreated smoothies. Both HPP significantly increased the extractability of lycopene, β-carotene and polyphenols compared to untreated samples. After HPP, ascorbic acid was retained by more than 92% of the initial content. The best results for antioxidant activity were obtained when HPP was applied at 600 MPa. FRAP and DPPH showed a high correlation with ascorbic acid (R(2) = 0.7135 and 0.8107, respectively) and polyphenolic compounds (R(2) = 0.6819 and 0.6935, respectively), but not with total carotenoids. Changes in bioactive compounds during the storage period were lower in the HPP smoothie than in the thermal-treated sample.

  12. A high performance liquid chromatography with ultraviolet method for Eschweilera nana leaves and their anti-inflammatory and antioxidant activities

    Science.gov (United States)

    Outuki, Priscila M.; Lazzeri, Nides S.; de Francisco, Lizziane M. B.; Bersani-Amado, Ciomar A.; Ferreira, Izabel C. P.; Cardoso, Mara Lane C.

    2015-01-01

    Background: Eschweilera nana Miers is a tree widely distributed in Cerrado, Brazil. Objective: In this study, we aimed to describe its phytochemical properties and antioxidant and topical anti-inflammatory effects for the first time, as well validate an high performance liquid chromatography with ultraviolet/visible (HPLC-UV-Vis) method for the separation and quantification of the main components (hyperoside and rutin) in the hydroalcoholic extract of E. nana leaves. Materials and Methods: Structural identification of compounds in E. nana extract was performed by analysis of spectral data by 1H nuclear magnetic resonance, 13C nuclear magnetic resonance and/or ESI/EM. The HPLC-UV-Vis method was validated according International Conference on Harmonization (ICH) parameters. The 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) method were used for determination of in vitro antioxidant activities and the croton oil-induced inflammation for evaluation of in vivo anti-inflammatory effects. Results: Hyperoside, rutin, α-amirin, β-amirin, β-sitosterol, and stigmasterol were identified in the hydroalcoholic extract of E. nana leaves. HPLC-UV-Vis was validated according to ICH parameters. Furthermore, in vitro and in vivo assays demonstrated that the hydroalcoholic extract and methanol fraction showed significant antioxidant and topical anti-inflammatory effects, as they were able to reduce ear edema induced by croton-oil application. Conclusions: This research showed the first phytochemical study of E. nana extract and their biological activities may be associated with the presence of flavonoids in the extracts. PMID:26246741

  13. Glucose tolerance and antioxidant activity of spent brewer's yeast hydrolysate with a high content of Cyclo-His-Pro (CHP).

    Science.gov (United States)

    Jung, Eun Young; Lee, Hyun-Sun; Choi, Jang Won; Ra, Kyung Soo; Kim, Mi-Ryung; Suh, Hyung Joo

    2011-03-01

    To elevate the Cyclo-His-Pro (CHP) content in yeast, the yeast hydrolysate that was obtained from enzymatic hydrolysis was subjected to various treatments. Flavourzyme-treated hydrolysate showed the highest CHP content (674.0 μg/g) among the various proteases treatments. Ultrafiltration was selected as the best method for concentrating CHP in yeast hydrolysate, based on the yields and CHP contents. In addition, we evaluated the radical scavenge and glucose tolerance of yeast hydrolysate with a high content of CHP. Yeast hydrolysate showed intense scavenging abilities of both 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals. The IC(50) values of yeast hydrolysate on DPPH and ABTS radicals were 1.9 and 0.9 mg/mL, respectively. There were significant differences in glucose level between the diabetes-control and yeast hydrolysate group at 30, 60, 90, and 120 min after injection in a type 1 diabetes model (P CHP as an antioxidative and/or antidiabetic material for the preparation of functional foods. This study tried to develop a material containing a high content of CHP using yeast for possible applications of this cyclic dipeptide in the therapy of metabolic disorders. The yeast hydrolysate prepared with Flavourzyme showed a high level of CHP. The hydrolysate with a high content of CHP showed high levels of radical scavenging activities and oral glucose tolerance activity. Therefore, it is possible to use the yeast hydrolysate with high levels of CHP as an antioxidative and/or antidiabetic material for the preparation of functional foods.

  14. Antioxidative activity of Geranium macrorrhizum

    NARCIS (Netherlands)

    Miliauskas, G.; Beek, van T.A.; Venskutonis, P.R.; Linssen, J.P.H.; Waard, de P.

    2004-01-01

    The composition of radical-scavenging compounds from Geranium macrorrhizum leaves was analyzed and the antioxidative activities of various extracts was determined. Seven compounds, namely gallic acid, ellagic acid, 4-galloyl quinic acid, the flavonoid quercetin and three of its glycosides, quercetin

  15. Different Citrus rootstocks present high dissimilarities in their antioxidant activity and vitamins content according to the ripening stage.

    Science.gov (United States)

    Cardeñosa, Vanessa; Barros, Lillian; Barreira, João C M; Arenas, Francisco; Moreno-Rojas, José M; Ferreira, Isabel C F R

    2015-02-01

    "Lane Late" sweet orange grafted on six different citrus rootstocks and grown in the Guadalquivir valley (Seville, Spain) were picked at different ripening stages in two consecutive seasons to characterize their antioxidant activity (free radicals scavenging activity, reducing power and lipid peroxidation inhibition) and quantify their main antioxidant compounds (vitamin E and vitamin C). Linear discriminant analysis and 2-way ANOVA were applied to compare the effects induced by citrus rootstock and ripening stage. The results showed that differences in antioxidant activity and related compounds are mainly dependent on the citrus rootstock, despite ripening stage had also some particular effects. Changes observed in 2012 showed less marked differences among the citrus rootstock. Nevertheless, Cleopatra rootstock showed the highest antioxidant activity in both years, indicating that an increase in its cultivation might be a good solution to sweet orange farmers. Concerning the ripening stage, samples collected in January presented higher vitamin contents, while those collected in April showed higher antioxidant activity. This result allows deciding the harvesting period according to the desired effect.

  16. Antioxidant activity, phenolic-flavonoid content and high-performance liquid chromatography profiling of three different variants of Syzygium cumini seeds: A comparative study

    Science.gov (United States)

    Priya, Syama Hari; Prakasan, Nisha; Purushothaman, Jayamurthy

    2017-01-01

    Background: The medicinally important phytochemicals present in Syzygium cumini seeds probably accounts for its wide use in traditional systems of medicines in India, like Ayurveda, Unani, and Siddha. Aim: The aim of the study was to determine the antioxidant potential of three different geographical variants of S. cumini seeds and to compare the phenolic profiling to know the effect of geographical variation in phenolic composition. Materials and Methods: Total phenolic and flavonoid content of S. cumini seeds were analyzed. Antioxidant activities in terms of 2,2-diphenyl-1-picrylhydrazyl, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), nitric oxide and superoxide radical scavenging assays were performed. The most active fractions were subjected to high-performance liquid chromatography (HPLC) profiling to identify the phenolic composition. Results: Among all the fractions, 70% methanol fraction of S. cumini seed showed significant antioxidant potential. There existed a linear correlation between phenolic content and antioxidant activity. HPLC profiling of 70% methanol (ME) fractions of all the variants revealed the presence of phenolic compounds with high concentrations of ellagic acid and gallic acid. The differences in phenolic concentration due to geographical changes might be the reason for higher antioxidant potential showed by 70% ME of Trivandrum variant. Conclusion: 70% methanolic fraction of S. cumini can act as a novel source of natural antioxidant. PMID:28163968

  17. Women with endometriosis improved their peripheral antioxidant markers after the application of a high antioxidant diet

    Directory of Open Access Journals (Sweden)

    Casanueva Esther

    2009-05-01

    Full Text Available Abstract Background Oxidative stress has been identified in the peritoneal fluid and peripheral blood of women with endometriosis. However, there is little information on the antioxidant intake for this group of women. The objectives of this work were 1 to compare the antioxidant intake among women with and without endometriosis and 2 to design and apply a high antioxidant diet to evaluate its capacity to reduce oxidative stress markers and improve antioxidant markers in the peripheral blood of women with endometriosis. Methods Women with (WEN, n = 83 and without endometriosis (WWE, n = 80 were interviewed using a Food Frequency Questionnaire to compare their antioxidant intake (of vitamins and minerals. Then, the WEN participated in the application of a control (n = 35 and high antioxidant diet (n = 37 for four months. The high antioxidant diet (HAD guaranteed the intake of 150% of the suggested daily intake of vitamin A (1050 μg retinol equivalents, 660% of the recommended daily intake (RDI of vitamin C (500 mg and 133% of the RDI of vitamin E (20 mg. Oxidative stress and antioxidant markers (vitamins and antioxidant enzymatic activity were determined in plasma every month. Results Comparison of antioxidant intake between WWE and WEN showed a lower intake of vitamins A, C, E, zinc, and copper by WEN (p Conclusion WEN had a lower intake of antioxidants in comparison to WWE. Peripheral oxidative stress markers diminished, and antioxidant markers were enhanced, in WEN after the application of the HAD.

  18. Antioxidant activity of potato juice

    Directory of Open Access Journals (Sweden)

    Przemysław Kowalczewski

    2012-06-01

    Full Text Available Background. The interest in potato juice as a therapeutic agent goes back to the 19th century but its application was not supported by any knowledge about biological activity of this raw material. Factors restricting the medical application of potato juice include its inattractive sensory and functional properties. The aim of the presented investigations was preliminary evaluation of the biological activity of potato juice and the impact on it of some technological operations such as: cryoconcentration and hydrolysis in a membrane reactor. Material and methods. Experiments comprised investigations of antioxidative potentials of fresh potato juice, products of its processing as well as fractions separated because of the size of their molecules using, for this purpose, Folin-Ciocalteu methods and reactions with the ABTS cation radical. Results. The value of the antioxidative potential of fresh potato juice determined by means of the ABTS reagent corresponded to approximately 330 μmol/100 g which is in keeping with literature data. As a result of the cryoconcentration process, the value determined by the Folin-Ciocalteu method was found to increase only slightly whereas the value determined by means of the ABTS reagent almost tripled. The antioxidative potential was found to grow even more strongly in the case of the application of both methods when the process of enzymatic hydrolysis was employed. The total of 5 protein fractions of molecular masses ranging from 11 000 Da to over 600 000 Da, as well as an organic non-protein fraction of the molecular mass of 600 Da, were obtained as a result of the performed separation. All the examined fractions exhibited antioxidative activities. The highest values determined by the Folin-Ciocalteu method were recorded for the protein fraction of 80 000 Da mean molecular mass, while using the ABTS reagent – for the organic, non-protein fraction. Conclusions. Potato juice possesses antioxidative activity which

  19. Rapid Identification and Comparison of Compounds with Antioxidant Activity in Coreopsis tinctoria Herbal Tea by High-Performance Thin-Layer Chromatography Coupled with DPPH Bioautography and Densitometry.

    Science.gov (United States)

    Lam, Shing-Chung; Lam, Sio-Fong; Zhao, Jing; Li, Shao-Ping

    2016-09-01

    A simple and efficient method based on high-performance thin-layer chromatography coupled with 2,2-diphenyl-1-picrylhydrazyl (DPPH) bioautography (HPTLC-DPPH) was established for the screening and comparison of antioxidants in different parts of Coreopsis tinctoria herbal tea from different origins and other related herbal tea materials, which used Chrysanthemum morifolium cv. "Gongju" and "Hangju" in this study. Scanning densitometry after DPPH derivatization was applied for the determination of antioxidant capacities of isolated compounds in each sample. It is considered that ethanol extracts of C. tinctoria had stronger antioxidant activity and more characteristic bands than those of 2 compared samples, C. morifolium cv. "Gongju" and "Hangju." Chemometric analysis results showed that the combination of hierarchical clustering analysis and principal component analysis based on determined antioxidant capacities could be used for the discrimination of different parts of C. tinctoria and C. morifolium. Results showed that 7 compounds made up the major contributions of antioxidant activity in C. tinctoria, including okanin, isookanin, marein, flavanomarein, 5,7,3',5'-tetrahydroxyflavanone-7-O-glucoside, 3,5-dicaffeoylquinic acid, and chlorogenic acid. Therefore, 7 compounds were identified as major antioxidant biomarkers for quality control of C. tinctoria. Results demonstrated that the established method could be applied for the identification of C. tinctoria, and were beneficial for the bioactivity-based quality control of C. tinctoria.

  20. Changes in extreme high-temperature tolerance and activities of antioxidant enzymes of sacred lotus seeds.

    Science.gov (United States)

    Ding, YanFen; Cheng, HongYan; Song, SongQuan

    2008-09-01

    Sacred lotus (Nelumbo nucifera Gaertn. 'Tielian') seed is long-lived and extremely tolerant of high temperature. Water content of lotus and maize seeds was 0.103 and 0.129 g H2O [g DW](-1), respectively. Water content, germination percentage and fresh weight of seedlings produced by surviving seeds gradually decreased with increasing treatment time at 100 degrees C. Germination percentage of maize (Zea mays L. 'Huangbaogu') seeds was zero after they were treated at 100 degrees C for 15 min and that of lotus seeds was 13.5% following the treatment at 100 degrees C for 24 h. The time in which 50% of lotus and maize seeds were killed by 100 degrees C was about 14.5 h and 6 min, respectively. With increasing treatment time at 100 degrees C, relative electrolyte leakage of lotus axes increased significantly, and total chlorophyll content of lotus axes markedly decreased. When treatment time at 100 degrees C was less than 12 h, subcellular structure of lotus hypocotyls remained fully intact. When treatment time at 100 degrees C was more than 12 h, plasmolysis gradually occurred, endoplasmic reticulum became unclear, nuclei and nucleoli broke down, most of mitochondria swelled, lipid granules accumulated at the cell periphery, and organelles and plasmolemma collapsed. Malondialdehyde (MDA) content of lotus axes and cotyledons decreased during 0 -12 h of the treatment at 100 degrees C and then increased. By contrast, the MDA content of maize embryos and endosperms increased during 5-10 min of the treatment at 100 degrees C and then decreased slightly. For lotus seeds: (1) activities of superoxide dismutase (SOD) and glutathione reductase (GR) of axes and cotyledons and of catalase (CAT) of axes increased during the early phase of treatment at 100 degrees C and then decreased; and (2) activities of ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR) of axes and cotyledons and of CAT of cotyledons gradually decreased with increasing treatment time at 100 degrees

  1. Changes in extreme high-temperature tolerance and activities of antioxidant enzymes of sacred lotus seeds

    Institute of Scientific and Technical Information of China (English)

    DING YanFen; CHENG HongYan; SONG SongQuan

    2008-01-01

    Sacred lotus (Nelumbo nucifera Gaertn. 'Tielian') seed is long-lived and extremely tolerant of high temperature. Water content of lotus and maize seeds was 0.103 and 0.129 g H2O [g DW]-1, respectively. Water content, germination percentage and fresh weight of seedlings produced by surviving seeds gradually decreased with increasing treatment time at 100℃. Germination percentage of maize (Zea mays L. 'Huangbaogu') seeds was zero after they were treated at 100℃ for 15 min and that of lotus seeds was 13.5% following the treatment at 100℃ for 24 h. The time in which 50% of lotus and maize seeds were killed by 100℃ was about 14.5 h and 6 min, respectively. With increasing treatment time at 100℃, relative electrolyte leakage of lotus axes increased significantly, and total chlorophyll content of lotus axes markedly decreased. When treatment time at 100℃ was less than 12 h, subcellular structure of lotus hypocotyls remained fully intact. When treatment time at 100℃ was more than 12 h, plasmoly-sis gradually occurred, endoplasmic reticulum became unclear, nuclei and nucleoli broke down, most of mitochondria swelled, lipid granules accumulated at the cell periphery, and organelles and plas-molemma collapsed. Malondialdehyde (MDA) content of lotus axes and cotyledons decreased during 0 -12 h of the treatment at 100℃ and then increased. By contrast, the MDA content of maize embryos and endosperms increased during 5-10 min of the treatment at 100℃ and then decreased slightly. For lotus seeds: (1) activities of superoxide dismutase (SOD) and glutathione reductase (GR) of axes and cotyledons and of catalase (CAT) of axes increased during the early phase of treatment at 100℃ and then decreased; and (2) activities of ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR) of axes and cotyledons and of CAT of cotyledons gradually decreased with increasing treat-ment time at 100℃. For maize seeds: (1) activities of SOD and DHAR of embryos and

  2. Changes in extreme high-temperature tolerance and activities of antioxidant enzymes of sacred lotus seeds

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Sacred lotus (Nelumbo nucifera Gaertn. ‘Tielian’) seed is long-lived and extremely tolerant of high temperature. Water content of lotus and maize seeds was 0.103 and 0.129 g H2O [g DW] ?1, respectively. Water content, germination percentage and fresh weight of seedlings produced by surviving seeds gradually decreased with increasing treatment time at 100℃. Germination percentage of maize (Zea mays L. ‘Huangbaogu’) seeds was zero after they were treated at 100℃ for 15 min and that of lotus seeds was 13.5% following the treatment at 100℃ for 24 h. The time in which 50% of lotus and maize seeds were killed by 100℃ was about 14.5 h and 6 min, respectively. With increasing treatment time at 100℃, relative electrolyte leakage of lotus axes increased significantly, and total chlorophyll content of lotus axes markedly decreased. When treatment time at 100℃ was less than 12 h, subcellular structure of lotus hypocotyls remained fully intact. When treatment time at 100℃ was more than 12 h, plasmoly-sis gradually occurred, endoplasmic reticulum became unclear, nuclei and nucleoli broke down, most of mitochondria swelled, lipid granules accumulated at the cell periphery, and organelles and plas-molemma collapsed. Malondialdehyde (MDA) content of lotus axes and cotyledons decreased during 0-12 h of the treatment at 100℃ and then increased. By contrast, the MDA content of maize embryos and endosperms increased during 5-10 min of the treatment at 100℃ and then decreased slightly. For lotus seeds: (1) activities of superoxide dismutase (SOD) and glutathione reductase (GR) of axes and cotyledons and of catalase (CAT) of axes increased during the early phase of treatment at 100℃ and then decreased; and (2) activities of ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR) of axes and cotyledons and of CAT of cotyledons gradually decreased with increasing treat-ment time at 100℃. For maize seeds: (1) activities of SOD and DHAR of embryos and

  3. Antioxidant Activity of Ethanol Extract of Pomegranate Seed

    Institute of Scientific and Technical Information of China (English)

    Yuzhong SHI; Yuan LU; Benguo LIU; Sasa ZUO

    2014-01-01

    In this study, the ethanol extract of pomegranate seed was prepared and its antioxidant activities were investigated. It was found the total phenolic content in the extract was as high as 41.791 mg GAE/g. And the extract showed high antioxi-dant activity measured as scavenging of DPPH radicals, hydroxyl radicals. It also exhibited strong antioxidant activity in reducing power and Rancimat test. These re-sults demonstrated Pomegranate seeds could serve as a new source of natural an-tioxidant.

  4. Determination of antioxidant content and antioxidant activity in foods using infrared spectroscopy and chemometrics: a review.

    Science.gov (United States)

    Lu, Xiaonan; Rasco, Barbara A

    2012-01-01

    Developing rapid analytical methods for bioactive components and predicting both the concentration and biological availability of nutraceutical components in foods is a topic of growing interest. Here, analysis of bioactive components and total antioxidant activity in food matrices using infrared spectroscopy coupled with chemometric predictive models is described. Infrared spectroscopy offers an alternative to wet chemistry, chromatographic determination of antioxidants, and in vitro biochemical assays for assessment of antioxidant activity. Spectroscopic methods provide a technique that can be used with biological tissues without extraction, which can often lead to degradation of the antioxidant components. Sample preparation time greatly decreases and analysis time is very short once a predictive model has been developed. Spectroscopic methods can have a high degree of precision when applied to analysis of nutraceutical compound concentration and antioxidant activity in foods. This article summarizes recent advances in vibrational spectroscopy and chemometrics and applications of these methods for antioxidant detection in foods.

  5. IN VITRO ANTIOXIDANT ACTIVITY OF IPOEMA BILOBA

    Directory of Open Access Journals (Sweden)

    Priti Tagde

    2012-01-01

    Full Text Available Biomolecules can be oxidized by free radicals. This oxidative damage has an important etiological role in aging and the development of diseases like cancer, atherosclerosis, and other inflammatory disorders. Synthetic antioxidants, like butylated hydroxyanisole, are good free radical scavengers; however, the synthetic antioxidants can be carcinogenic. Therefore, there is an increasing interest in searching for antioxidants of natural origin.   Antioxidants with different chemical properties may recharge each other in an antioxidant network. The total antioxidant content of dietary plants may therefore be a useful tool for testing the 'antioxidant network' hypothesis. Several berries, fruits, nuts, seeds, vegetables, drinks and spices have been found to be high in total antioxidants. Initial studies in animals and humans are supportive as to the beneficial effects of dietary plants rich in total antioxidants. Additionally, antioxidants and other plant compounds may also improve the endogenous antioxidant defense through induction of antioxidant and phase 2 enzymes.1,2 Dietary plants rich in such compounds include broccoli, brussel sprouts, cabbage, kale, cauliflower, carrots, onions, tomatoes, spinach and garlic , antioxidants and other plant compounds may also improve the endogenous antioxidant defense through induction of antioxidant and phase 2 enzymes.

  6. Effect of high hydrostatic pressure on phenolic compounds, ascorbic acid and antioxidant activity in cashew apple juice

    Science.gov (United States)

    Queiroz, C.; Moreira, C. F. F.; Lavinas, F. C.; Lopes, M. L. M.; Fialho, E.; Valente-Mesquita, V. L.

    2010-12-01

    The cashew apple is native to Brazil, but there is insufficient information regarding the nutritional properties of this fruit. The objective of this study was to evaluate the impact of high pressure processing (HPP) at room temperature (25 °C) on phenolic compound and ascorbic acid contents and antioxidant capacity of cashew apple juice. This study showed that HPP at 250 or 400 MPa for 3, 5 and 7 min did not change pH, acidity, total soluble solids, ascorbic acid or hydrolysable polyphenol contents. However, juice pressurized for 3 and 5 min showed higher soluble polyphenol contents. Antioxidant capacity, measured by the ferric-reducing antioxidant power method, was not altered by HPP, but when treated at 250 MPa for 3 min, it resulted in an increased value when 2,2-diphenyl-1-picrylhydrazyl was used. These data demonstrate that HPP can be used in the food industry for the generation of products with higher nutritional quality.

  7. Antioxidative Activity of Tobacco Leaf Protein Hydrolysates

    Directory of Open Access Journals (Sweden)

    Guohua Rao

    2007-01-01

    Full Text Available Discarded tobacco leaf protein hydrolysate (DTLPH was prepared by enzymatic hydrolysis using papain and then separated using ultrafiltration (UF membranes with molecular mass cut-off (MMCO of 10, 5, 3 and 1 kDa. Four permeate fractions including 10-K, 5-K, 3-K and 1-K (the permeate fractions from 10, 5, 3 and 1 kDa hydrolysate fractions were obtained. The 5-K hydrolysate fraction had high oxidation inhibilitory ratio (42.62 %, which was about twofold higher than the original hydrolysate and as high as that of vitamin E (α-tocopherol. The fractionated hydrolysates were superior to the original hydrolysate in the antioxidative activity tested. Moreover, these separated hydrolysates showed the enhanced functional property. The amino acid composition of 5-K hydrolysate was analyzed and the results show that the high antioxidative activity of 5-K hydrolysate was derived from high content of histidine, methionine, cystine and tryptophan.

  8. Antioxidant activity of Sphaerococcus coronopifolius associated bacteria

    Directory of Open Access Journals (Sweden)

    Nádia Fino

    2014-06-01

    Full Text Available Associated bacteria living on macroalgae surfaces are an interesting source of new secondary metabolites with biological activities. The aim of this study was the isolation and identification of epiphytic bacteria from the marine algae Sphaerococcus coronopifolius and the evaluation of the antioxidant activity of the bacteria extracts. The identification of epiphytic bacteria was determined by 16S rRNA gene sequencing. Bacteria extracts were obtained with methanol and dichloromethane (1:1 extraction. Antioxidant activity was evaluated by quantification of total phenolic content (TPC, 2,2-diphenyl-1-picrylhydrazyl (DPPH radical scavenging activity and oxygen radical absorbent capacity (ORAC. The extracts with higher antioxidant activity were tested on MCF-7 and HepG-2 cell lines in oxidative stress conditions induced by H2O2 at 0.2 mM and 0.5 mM, respectively. In total were isolated 21 Sphaerococcus coronopifolius associated bacteria and identified as Vibrio sp. (28.57%, Shewanella sp. (23.81%, Pseudoalteromonas sp. (19.05%, Bacillus sp. (9.52% and Halomonas sp. (9.52%. Two (9.52% of them presented less than 90% Basic Local Alignment Search Tool (BLAST match. The epiphytic bacteria with the most antioxidant potential evaluated by ORAC and DPPH methods were Sp2, Sp12, Sp23, Sp25 and Sp27. The strain Sp4 show high antioxidant activity in all antioxidant methods (ORAC, DPPH and TPC. In oxidative stress conditions on MCF-7 cell line, the extracts of bacteria (1mg.ml-1: 24hours Sp4 (16.15%, Sp25 (17.95% and Sp27 (10.65% prevented the cell death induced by H2O2. In the HepG-2 cell line was the extracts of Sp2 (9.01%, Sp4 (11.21%, Sp12 (7.20% and Sp23 (8.81% bacteria that high prevented the oxidative stress condition induced by H2O2. In conclusion, the Sphaerococcus coronopifolius associated bacteria can be an interesting and excellent source of marine natural compounds with antioxidant activity.

  9. Antioxidant activity of the microalga Spirulina maxima

    OpenAIRE

    Miranda, M. S.; Cintra,R.G.; S.B.M. Barros; J. Mancini-Filho

    1998-01-01

    Spirulina maxima, which is used as a food additive, is a microalga rich in protein and other essential nutrients. Spirulina contains phenolic acids, tocopherols and ß-carotene which are known to exhibit antioxidant properties. The aim of the present study was to evaluate the antioxidant capacity of a Spirulina extract. The antioxidant activity of a methanolic extract of Spirulina was determined in vitro and in vivo. The in vitro antioxidant capacity was tested on a brain homogenate incubated ...

  10. Antioxidative activity of Silibum marianum cultivated in Mongolia

    Directory of Open Access Journals (Sweden)

    J Ganbaatar

    2014-12-01

    Full Text Available Silibum marianum (Milk thistle contains high amount of phenolic compounds with antioxidant properties. The aim of this work was to evaluate the antioxidant activity of  phenolic compounds included in Milk thistle. The  antioxidant properties of the leaves and seeds of milk thistle were examined by determining its ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl (DPPH free radical. The results obtained from DPPH assay confirm that Milk thistle extracts have high antioxidative activity.DOI: http://doi.dx.org/10.5564/mjc.v15i0.323 Mongolian Journal of Chemistry 15 (41, 2014, p53-55

  11. ANTIOXIDANT ACTIVITIES AND PHENOLIC PROFILE OF SIX MOROCCAN SELECTED HERBS

    Directory of Open Access Journals (Sweden)

    Madiha Bichra

    2013-02-01

    Full Text Available The present work evaluated the antioxidant capacity of six plants commonly used in traditional Moroccan medicine. The antioxidant capacity was estimated by DPPH test, ferrous ion chelating activity and ABTS test. As results, the highest antioxidant activities were found in Mentha suaveolens, Salvia officinalis and Mentha viridis. Different species showed significant differences in their total phenolic content (TPC. The highest level of phenolics was found in Salvia officinalis and the lowest in Pelargonium roseum. Linear correlation was found between TPC, especially the non-flavonoid content (NFC and the antioxidant activity. Qualitative and quantitative analyzes of major phenolics by reverse-phase high-performance liquid chromatography (RP-HPLC were also performed. On the basis of the obtained results, these studied medicinal herbs were found to serve as a potential source of natural antioxidants due to their richness in phenolic compounds and marked antioxidant activity.

  12. Antioxidant activity assays on-line with liquid chromatography

    NARCIS (Netherlands)

    Niederlander, Harm A. G.; van Beek, Teris A.; Bartasiute, Aiste; Koieva, Irina I.

    2008-01-01

    Screening for antioxidants requires simple in vitro model systems to investigate antioxidant activity. High resolution screening (HRS), combining a separation technique like HPLC with fast post-column (bio)chemical detection can rapidly pinpoint active compounds in complex mixtures. In this paper bo

  13. Fingerprint analysis, multi-component quantitation, and antioxidant activity for the quality evaluation of Salvia miltiorrhiza var. alba by high-performance liquid chromatography and chemometrics.

    Science.gov (United States)

    Zhang, Danlu; Duan, Xiaoju; Deng, Shuhong; Nie, Lei; Zang, Hengchang

    2015-10-01

    Salvia miltiorrhiza Bge. var. alba C.Y. Wu and H.W. Li has wide prospects in clinical practice. A useful comprehensive method was developed for the quality evaluation of S. miltiorrhiza var. alba by three quantitative parameters: high-performance liquid chromatography fingerprint, ten-component contents, and antioxidant activity. The established method was validated for linearity, precision, repeatability, stability, and recovery. Principal components analysis and hierarchical clustering analysis were both used to evaluate the quality of the samples from different origins. The results showed that there were category discrepancies in quality of S. miltiorrhiza var. alba samples according to the three quantitative parameters. Multivariate linear regression was adopted to explore the relationship between components and antioxidant activity. Three constituents, namely, danshensu, rosmarinic acid, and salvianolic acid B, significantly correlated with antioxidant activity, and were successfully elucidated by the optimized multivariate linear regression model. The combined use of high-performance liquid chromatography fingerprint analysis, simultaneous multicomponent quantitative analysis, and antioxidant activity for the quality evaluation of S. miltiorrhiza var. alba is a reliable, comprehensive, and promising approach, which might provide a valuable reference for other herbal products in general to improve their quality control.

  14. Comparison and Characterization of Compounds with Antioxidant Activity in Lycium barbarum Using High-Performance Thin Layer Chromatography Coupled with DPPH Bioautography and Tandem Mass Spectrometry.

    Science.gov (United States)

    Lam, Shing-Chung; Luo, Zhen; Wu, Ding-Tao; Cheong, Kit-Leong; Hu, De-Jun; Xia, Zu-Meng; Zhao, Jing; Li, Shao-Ping

    2016-06-01

    Methanol extracts from 50 batches of Lycium barbarum (L. barbarum, wolfberry) in China were compared and characterized using high-performance thin-layer chromatography coupled with 2,2-diphenyl-1-picrylhydrazyl (DPPH) bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS), respectively. Results showed that similar components occupying the major antioxidant activity existed in L. barbarum collected from different origins. However, the average antioxidant capacities of methanol extracts of L. barbarum collected in Ningxia were significantly higher than those of Qinghai, Xinjiang, Inner Mongolia, and Gansu, which may contribute to rational use of L. barbarum in China. Furthermore, the chemical structure of compound with the highest antioxidant capacity was tentatively identified as 2-O-β-d-glucopyranosyl-l-ascorbic acid using ESI-Q-TOF-MS/MS analysis, which possessed high potentials to be used as an antioxidant biomarker for the quality control of L. barbarum. Results are helpful for the bioactivity-based quality control of L. barbarum, and beneficial for the improvement of their performance in functional/health foods area, suggesting that HPTLC-DPPH bioautography with ESI-Q-TOF-MS/MS could be used as a routine approach for quality control of antioxidant components in L. barbarum. © 2016 Institute of Food Technologists®

  15. Cytotoxic and antioxidant activity of selected marine sponges

    Directory of Open Access Journals (Sweden)

    Chairman K

    2012-06-01

    Full Text Available Objective: To evaluate the anticancer activity of the crude extracts of Rhabdastrella globostellata (R. globostellata and Spirastrella inconstans (S. inconstans var. moeandrina Dendy. Methods: Soxhlet extraction method was used to extract the secondary metabolites and various assays antioxidant, anticancer and various assays were carried out. The extract were tested anticancer activity against a HeLa, Raw 264.7 and Hek-293. Results: The sponge extracts tested exhibited from median to high toxicity in at least one of the toxicity bioassays performed. The antioxidant activity of the isolated metabolite in ethylacetate solution was assessed by SOD and GTH assays and compared with that of other known natural antioxidants. Conclusions: Potent antioxidants have been detected among both phenolic metabolites and alkaloids. Antioxidant effects of tested compounds have been attributed to their action as chain-breaking antioxidants and/or as scavengers of radicals

  16. Innate antioxidant activity of some traditional formulations

    Directory of Open Access Journals (Sweden)

    Gunpreet Kaur

    2017-01-01

    Full Text Available Herbal medicine is the oldest form of healthcare known to humanity. Recently, much attention is being directed toward the use of antioxidants. There are some very commonly used Ayurvedic preparations that might have inbuilt antioxidant activity, and their therapeutic potential can be partially attributable to its antioxidant activity. Hence, it was proposed to find out antioxidant activity of such common formulations. Estimation of innate antioxidant activity of some commonly used traditional formulations. In this study, five formulations were evaluated for antioxidant activity in comparison to gallic acid (standard using the in vitro reducing power method and superoxide radical scavenging activity by dimethyl sulfoxide method followed by calculation of scavenging activity and inhibitory concentration 50% (IC 50 . The result shows that Ayurvedic drug extracts possess good reducing power and antioxidant activity. Laxmivilas Ras shows higher reducing power ranging from 117 ± 0.021 to 0.176 ± 0.012 as compared to other extracts. The drug extracts were also found to be an efficient scavenger of superoxide radical. The IC 50 values for Laxmivilas Ras, Agnitundi Vati, Ajmodadi Churna, Tribhuvankirti Rasa, gallic acid (standard and Sitopladi Churna, were found to be 50.07, 98.41, 105.13, 116.39, 176.80, and 200.17, respectively. From this study, it can be concluded that the above Ayurvedic formulations possess antioxidant property. However, work could be initiated on the isolation and identification of these antioxidant components.

  17. Synthesis and Antioxidant Activity of Sapriparaquinone Analogues

    Institute of Scientific and Technical Information of China (English)

    Fei DENG; Fu Jun ZHANG; Ming ZHAO; Yi Ping WANG; Jin Sheng ZHANG

    2006-01-01

    Twenty two sapriparaquinone derivatives were synthesized and their antioxidant activities were evaluated in vitro. Many of this kind of compounds demonstrated potent antioxidant activity against lipid peroxidation, especially compound 7 (ICs0 = 3.7 μg/mL). The preliminary structure-activity relationship of sapriparaquinone derivatives was discussed.

  18. Evaluation of Antioxidant Activity of Some Pteridophytes

    Directory of Open Access Journals (Sweden)

    Amit Semwal

    2013-04-01

    Full Text Available The present study was undertaken to find the antioxidant value of certain Pteridophytes in Garhwalregion. Antioxidants have been reported to prevent oxidative damage caused by free radical and can be used in cardiovascular and anti-inflammatory diseases to treat of burn and wounds. The methanolic crude extracts of some commonly used Pteridophytes were screened for their free radical scavengingproperties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH free radical. The overall antioxidant activity of Diplaziumesculantum was the strongest, followed in descending order by Adiantumlunulatum, Pterisvittata, Equisetum romosissimumand Ampelopterisprolifera. All the methanolicextracts exhibited antioxidant activity significantly. The IC50 of the methanolic extracts ranged between 0.32 ± 0.12 and 0.81 ± 0.21 mg/ml. The study reveals that the consumption of these spices would exert several beneficial effects by virtue of their antioxidant activity.

  19. [Antioxidant activity of flaxseed oil].

    Science.gov (United States)

    Prozorovskaia, N N; Rusina, I F; Lupinovich, V L; Beketova, N A; Sorokin, I V; Ipatova, O M; Levachev, M M

    2003-01-01

    Effective concentration of antioxidants and its reactivity toward peroxil radicals (constant k7) have been measured by the chemiluminescence technique for flaxseed oil. Effective concentration of antioxidants is shown to depend on the technology of producing flaxseed oil; period of seed storage before use; and storing duration of flaxseed oil also. Minor component content of flaxseed oil, which may be the members of antioxidant pool, has been quantitatively estimated.

  20. Antioxidant activity of lichen Cetraria aculeata

    Directory of Open Access Journals (Sweden)

    Tomović Jovica

    2016-01-01

    Full Text Available The aim of the present study is to investigate the antioxidant properties of the lichen Cetraria aculeata. Antioxidant activity of the methanol and ethyl acetate extracts of lichen was tested by different methods including determination of total phenolics content, determination of total antioxidant capacity, DPPH free radical scavenging activity, inhibitory activity towards lipid peroxidation, ferrous ion chelating ability and hydroxyl radical scavenging activity. The extracts of the lichen C. aculeata showed significant antioxidant activity. The methanol extract showed higher values for total phenolics and total antioxidant capacity compared to the ethyl acetate extract, while the ethyl acetate extract demonstrated better results for DPPH radical scavenging, inhibitory activity towards lipid peroxidation, chelating ability and hydroxyl radical scavenging than the methanol extract. This is the first report of the antioxidant properties of Cetraria aculeata growing in Serbia. The results of antioxidant activity indicate the application of this lichen as source of natural antioxidants that could be used as a possible food supplement, in the pharmaceutical industry and in the treatment of various diseases.

  1. Antioxidant activity of wine assessed by different in vitro methods

    Directory of Open Access Journals (Sweden)

    Di Lorenzo Chiara

    2017-01-01

    Full Text Available Epidemiological studies have suggested that a diet rich in antioxidant compounds could help in counteracting the effects of reactive oxygen species, reducing the risk factors for chronic diseases. The moderate consumption of wine, especially red wine, has been associated with the reduction in mortalities from cardiovascular diseases. One of the possible reasons for the protective effect of wine can be identified in the high content of polyphenols (mainly flavonoids, which have significant antioxidant activity. Even though several in vitro tests have been developed for the measure of the antioxidant property, no method has showed a satisfactory correlation with the in vivo situation. On these bases, the aim of this study was the application and comparison of different in vitro methods to assess the antioxidant activity of red, rosé and white wines. The methods were: 1 Folin-Cocalteau's assay for the quantification of total polyphenol content; 2 the DPPH (1,1-diphenyl-2-picrylhydrazyl spectrophotometric assay and the Trolox Equivalent Antioxidant Capacity (TEAC spectrophotometric assay for measuring the antioxidant activity of samples; 3 High Performance Thin Layer Chromatography for separation of phenolic substances and assessment of the associated antioxidant activity; 4 electrochemical detection by using a biosensor. Although all the approaches show some limitations, this battery of tests offers a more reliable body of data on the antioxidant activity of vine derivatives.

  2. Tannins and Antioxidant Activities of the Walnut (Juglans regia) Pellicle.

    Science.gov (United States)

    Yin, Tian-Peng; Cai, Le; Chen, Yang; Li, Ying; Wang, Ya-Rong; Liu, Chuan-Shui; Ding, Zhong-Tao

    2015-12-01

    The total phenolic content and antioxidant activities of the acetone extract and derived fractions from the walnut (Juglans regia) pellicle were estimated. The BuOH fraction exhibited the strongest antioxidant activity with the highest phenolic content. A phytochemical investigation of this fraction led to the isolation of three tannins, 2,3-hexahydroxydiphenoylglucose (1), pedunculagin (2) and 2,3,4,6-tetragalloylglucose (3). Pedunculagin showed high content and powerful activity, which implied that this compound plays an important role in the antioxidant activity of the walnut pellicle.

  3. Cellular antioxidant activity of common vegetables.

    Science.gov (United States)

    Song, Wei; Derito, Christopher M; Liu, M Keshu; He, Xiangjiu; Dong, Mei; Liu, Rui Hai

    2010-06-01

    The measurement of antioxidant activity using biologically relevant assays is important to screen fruits, vegetables, natural products, and dietary supplements for potential health benefits. The cellular antioxidant activity (CAA) assay quantifies antioxidant activity using a cell culture model and was developed to meet the need for a more biologically representative method than the popular chemistry antioxidant capacity measures. The objective of the study was to determine the CAA, total phenolic contents, and oxygen radical absorbance capacity (ORAC) values of 27 vegetables commonly consumed in the United States. Beets, broccoli, and red pepper had the highest CAA values, whereas cucumber had the lowest. CAA values were significantly correlated to total phenolic content. Potatoes were found to be the largest contributors of vegetable phenolics and CAA to the American diet. Increased fruit and vegetable consumption is an effective strategy to increase antioxidant intake and decrease oxidative stress and may lead to reduced risk of developing chronic diseases, such as cancer and cardiovascular disease.

  4. Antioxidant activity of selected Indian spices.

    Science.gov (United States)

    Shobana, S; Naidu, K A

    2000-02-01

    Spices and vegetables possess antioxidant activity that can be applied for preservation of lipids and reduce lipid peroxidation in biological systems. The potential antioxidant activities of selected spices extracts (water and alcohol 1:1) were investigated on enzymatic lipid peroxidation. Water and alcoholic extract (1:1) of commonly used spices (garlic, ginger, onion, mint, cloves, cinnamon and pepper) dose-dependently inhibited oxidation of fatty acid, linoleic acid in presence of soybean lipoxygenase. Among the spices tested, cloves exhibited highest while onion showed least antioxidant activity. The relative antioxidant activities decreased in the order of cloves, cinnamon, pepper, ginger, garlic, mint and onion. Spice mix namely ginger, onion and garlic; onion and ginger; ginger and garlic showed cumulative inhibition of lipid peroxidation thus exhibiting their synergistic antioxidant activity. The antioxidant activity of spice extracts were retained even after boiling for 30 min at 100 degrees C, indicating that the spice constituents were resistant to thermal denaturation. The antioxidant activity of these dietary spices suggest that in addition to imparting flavor to the food, they possess potential health benefits by inhibiting the lipid peroxidation.

  5. Antioxidant, antimicrobial and antiproliferative activities of Anthemis palestina essential oil

    National Research Council Canada - National Science Library

    Bardaweel, Sanaa K; Tawaha, Khaled A; Hudaib, Mohammad M

    2014-01-01

    .... The antioxidant activity of the hydrodistilled oil was characterized using various in vitro model systems such as DPPH, ferric-reducing antioxidant power and hydroxyl radical scavenging activity...

  6. Comparative analysis of caffeoylquinic acids and lignans in roots and seeds among various burdock (Arctium lappa) genotypes with high antioxidant activity.

    Science.gov (United States)

    Liu, Jingyi; Cai, Yi-Zhong; Wong, Ricky Ngok Shun; Lee, Calvin Kai-Fai; Tang, Sydney Chi Wai; Sze, Stephen Cho Wing; Tong, Yao; Zhang, Yanbo

    2012-04-25

    Caffeoylquinic acids and lignans in the crude extracts of both roots and seeds from different burdock ( Arctium lappa L.) genotypes were simultaneously characterized and systematically compared by LC-MS and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS), and their antioxidant activities were also investigated. A total of 14 lignans were identified in burdock seeds and 12 caffeoylquinic acids in burdock roots. High levels of caffeoylquinic acids were also detected in burdock seeds, but only trace amounts of lignans were found in burdock roots. Burdock seeds contained higher concentrations of lignans and caffeoylquinic acids than burdock roots. Quantitative analysis of caffeoylquinic acids and lignans in roots and seeds of various burdock genotypes was reported for the first time. Great variations in contents of both individual and total phenolic compounds as well as antioxidant activities were found among different genotypes. Burdock as a root vegetable or medicinal plants possessed considerably stronger antioxidant activity than common vegetables and fruits.

  7. In vitro anti-oxidative activities of the various parts of Parkia biglobosa and GC-MS analysis of extracts with high activity.

    Science.gov (United States)

    Ibrahim, M A; Koorbanally, N A; Islam, M S

    2013-01-01

    The anti-oxidative activities of sequentially extracted solvent fractions of different parts of P. biglobosa were evaluated in a series of in vitro assays. Our findings indicated that all extracts had electron donating and free radical scavenging activities. But the ethanol (EtOH) extracts from all the parts demonstrated more promising anti-oxidative effects in these experimental models. Apart from the aqueous extracts of the stem bark and leaves, all other extracts exhibited hydroxyl radical scavenging (HRS) activity but the ethyl acetate (EtOAc) extract of the stem bark and EtOH extracts of the root and leaves possessed more powerful HRS activity than other corresponding extracts in the parts. Further, nitric oxide (NO) inhibition activities were observed in all the extracts except the EtOAc extract of the stem bark which showed pro-oxidative activity. However, the EtOH extract of the stem bark and root as well as the EtOAc extract of the leaves displayed more potent anti-NO activity than other extracts in the parts. The GC-MS analysis of the EtOH extracts revealed that the most abundant phytochemicals are pyrogallol derivatives. Data from this study suggest that the EtOH extracts from different parts of P. biglobosa contained potent anti-oxidative agents and pyrogallol could be the main bioactive constituent.

  8. Scuba diving activates vascular antioxidant system.

    Science.gov (United States)

    Sureda, A; Batle, J M; Ferrer, M D; Mestre-Alfaro, A; Tur, J A; Pons, A

    2012-07-01

    The aim was to study the effects of scuba diving immersion on plasma antioxidant defenses, nitric oxide production, endothelin-1 and vascular endothelial growth factor levels. 9 male divers performed an immersion at 50 m depth for a total time of 35 min. Blood samples were obtained before diving at rest, immediately after diving, and 3 h after the diving session. Leukocyte counts, plasma 8oxoHG, malondialdehyde and nitrite levels significantly increased after recovery. Activities of lactate dehydrogenase, creatine kinase, catalase and superoxide significantly increased immediately after diving and these activities remained high after recovery. Plasma myeloperoxidase activity and protein levels and extracellular superoxide dismutase protein levels increased after 3 h. Endothelin-1 concentration significantly decreased after diving and after recovery. Vascular endothelial growth factor concentration significantly increased after diving when compared to pre-diving values, returning to initial values after recovery. Scuba diving at great depth activated the plasma antioxidant system against the oxidative stress induced by elevated pO₂ oxygen associated with hyperbaria. The decrease in endothelin-1 levels and the increase in nitric oxide synthesis could be factors that contribute to post-diving vasodilation. Diving increases vascular endothelial growth factor plasma levels which can contribute to the stimulation of tissue resistance to diving-derived oxidative damage.

  9. High performance liquid chromatography-mass spectrometry analysis of high antioxidant australian fruits with antiproliferative activity against cancer cells

    Directory of Open Access Journals (Sweden)

    Joseph Sirdaarta

    2016-01-01

    Abbreviations used: DPPH: di (phenyl- (2,4,6-trinitrophenyl iminoazanium, HPLC: High-performance liquid chromatography, IC50: The concentration required to inhibit by 50%, LC50: The concentration required to achieve 50% mortality, MS: Mass spectrometry. Ian Edwin Cock

  10. Antioxidant activity of the microalga Spirulina maxima

    Directory of Open Access Journals (Sweden)

    M.S. Miranda

    1998-08-01

    Full Text Available Spirulina maxima, which is used as a food additive, is a microalga rich in protein and other essential nutrients. Spirulina contains phenolic acids, tocopherols and ß-carotene which are known to exhibit antioxidant properties. The aim of the present study was to evaluate the antioxidant capacity of a Spirulina extract. The antioxidant activity of a methanolic extract of Spirulina was determined in vitro and in vivo. The in vitro antioxidant capacity was tested on a brain homogenate incubated with and without the extract at 37oC. The IC50 (concentration which causes a 50% reduction of oxidation of the extract in this system was 0.18 mg/ml. The in vivo antioxidant capacity was evaluated in plasma and liver of animals receiving a daily dose of 5 mg for 2 and 7 weeks. Plasma antioxidant capacity was measured in brain homogenate incubated for 1 h at 37oC. The production of oxidized compounds in liver after 2 h of incubation at 37oC was measured in terms of thiobarbituric acid reactant substances (TBARS in control and experimental groups. Upon treatment, the antioxidant capacity of plasma was 71% for the experimental group and 54% for the control group. Data from liver spontaneous peroxidation studies were not significantly different between groups. The amounts of phenolic acids, a-tocopherol and ß-carotene were determined in Spirulina extracts. The results obtained indicate that Spirulina provides some antioxidant protection for both in vitro and in vivo systems.

  11. Effect of Partial Replacement of Wheat Flour with High Quality Cassava Flour on the Chemical Composition, Antioxidant Activity, Sensory Quality, and Microbial Quality of Bread

    Science.gov (United States)

    Eleazu, Chinedum; Eleazu, Kate; Aniedu, Chinyere; Amajor, John; Ikpeama, Ahamefula; Ebenzer, Ike

    2014-01-01

    In the current study, wheat flour was mixed with high quality cassava flour (HQCF) in several ratios: 90:10, 80:20, 70:30, and 60:40, and used to prepare 10%, 20%, 30%, and 40% National Root Crops Research Institute (NRCRI) cassava bread, respectively. 100% wheat bread was prepared as a control (100% wheat bread). Five bread samples were prepared per group. Antioxidant assays [i.e., 2,2-diphenyl- 1-picrylhydrazyl radical (DPPH) scavenging assay, reducing power assay] revealed that the bread samples had considerable antioxidant capacities. Substitution of wheat flour with HQCF at various concentrations resulted in dose dependent decreases in the mineral and protein contents of the resulting bread samples. The crude fiber content of the bread samples was minimal, while the carbohydrate content of the bread samples ranged from 43.86% to 48.64%. A 20% substitution of wheat flour with HQCF yielded bread samples with a general acceptability that was comparable to that of 100% wheat bread. The mean bacteria counts of the bread samples ranged from 2.0×103 CFU/mL to 1.4×104 CFU/mL, while the fungal counts ranged from 0 CFU/mL to 3×103 CFU/mL. There was a positive correlation between the DPPH antioxidant activities and the reducing powers of the bread samples (R2=0.871) and a positive correlation between the DPPH antioxidant activities and the flavonoid contents of the bread samples (R2=0.487). The higher microbial load of the NRCRI cassava bread samples indicates that these bread samples may have a shorter shelf life than the 100% wheat bread. The significant positive correlation between total flavonoid content and reducing power (R2=0.750) suggests that the flavonoids present in the lipophilic fractions of the bread samples could be responsible for the reductive capacities of the bread samples. PMID:25054110

  12. Antioxidant activity of Syzygium cumini leaf gall extracts

    Directory of Open Access Journals (Sweden)

    Ravi Shankara Birur Eshwarappa

    2014-06-01

    Full Text Available Introduction: Free radicals are implicated in several metabolic diseases and the medicinal properties of plants have been explored for their potent antioxidant activities to counteract metabolic disorders. This research highlights the chemical composition and antioxidant potential of leaf gall extracts (aqueous and methanol of Syzygium cumini S. cumini, which have been extensively used in traditional medications to treat various metabolic diseases. Methods: The antioxidant activities of leaf gall extracts were examined using diphenylpicrylhydrazyl (DPPH, nitric oxide scavenging, hydroxyl scavenging and ferric reducing power (FRAP methods. Results: In all the methods, the methanolic extract showed higher antioxidant potential than the standard ascorbic acid. The presence of phenolics, flavonoids, phytosterols, terpenoids, and reducing sugars was identified in both the extracts. When compared, the methanol extract had the highest total phenolic and flavonoid contents at 474±2.2 mg of GAE/g d.w and 668±1.4 mg of QUE/g d.w, respectively. The significant high antioxidant activity can be positively correlated to the high content of total polyphenols/flavonoids of the methanol extract. Conclusion: The present study confirms the folklore use of S. cumini leaves gall extracts as a natural antioxidant and justifies its ethnobotanical use. Further, the result of antioxidant properties encourages the use of S. cumini leaf gall extracts for medicinal health, functional food and nutraceuticals applications.

  13. Antioxidant Activities of Extracts and Fractions from Eupatorium lindleyanum DC

    Directory of Open Access Journals (Sweden)

    Yonghong Hu

    2011-07-01

    Full Text Available The antioxidant activities of water extract (WE, ethanol extract (EE, residue water extract (RWE and petroleum ether (PF, ethyl acetate (EF, n-BuOH (BF and water (WF fractions of the ethanol extract from Eupatorium Lindley DC were investigated for the first time. Total phenolics content, DPPH radical scavenging activities, superoxide radical scavenging activities, total reduction capability, and ferrous ions chelating activities were determined for all the extracts and fractions. The results showed that all the extracts and fractions exhibited antioxidant activities with different magnitudes of potency. Among all the samples, WE and RWE exhibited the best antioxidant capacities, the BF also exhibited high antioxidant abilities in all tests except for the metal chelating activity, while the other extracts and fractions were relatively weak antioxidants. The BF had the highest total phenolics contents in all extracts and fractions, and the WE and RWE were found to be rich in tannins. Furthermore, the content of total phenolics showed good correlation with DPPH radical scavenging activity, superoxide anion radical scavenging activity, and the reducing power. Phenolic composition of all the extracts and fractions was identified and quantified by HPLC. The results indicate that the extracts of E. Lindley DC might be a useful potential source of natural antioxidant ingredients.

  14. Antioxidant Activity of Hawaiian Marine Algae

    Directory of Open Access Journals (Sweden)

    Anthony D. Wright

    2012-02-01

    Full Text Available Marine algae are known to contain a wide variety of bioactive compounds, many of which have commercial applications in pharmaceutical, medical, cosmetic, nutraceutical, food and agricultural industries. Natural antioxidants, found in many algae, are important bioactive compounds that play an important role against various diseases and ageing processes through protection of cells from oxidative damage. In this respect, relatively little is known about the bioactivity of Hawaiian algae that could be a potential natural source of such antioxidants. The total antioxidant activity of organic extracts of 37 algal samples, comprising of 30 species of Hawaiian algae from 27 different genera was determined. The activity was determined by employing the FRAP (Ferric Reducing Antioxidant Power assays. Of the algae tested, the extract of Turbinaria ornata was found to be the most active. Bioassay-guided fractionation of this extract led to the isolation of a variety of different carotenoids as the active principles. The major bioactive antioxidant compound was identified as the carotenoid fucoxanthin. These results show, for the first time, that numerous Hawaiian algae exhibit significant antioxidant activity, a property that could lead to their application in one of many useful healthcare or related products as well as in chemoprevention of a variety of diseases including cancer.

  15. Antioxidant activity of Hawaiian marine algae.

    Science.gov (United States)

    Kelman, Dovi; Posner, Ellen Kromkowski; McDermid, Karla J; Tabandera, Nicole K; Wright, Patrick R; Wright, Anthony D

    2012-02-01

    Marine algae are known to contain a wide variety of bioactive compounds, many of which have commercial applications in pharmaceutical, medical, cosmetic, nutraceutical, food and agricultural industries. Natural antioxidants, found in many algae, are important bioactive compounds that play an important role against various diseases and ageing processes through protection of cells from oxidative damage. In this respect, relatively little is known about the bioactivity of Hawaiian algae that could be a potential natural source of such antioxidants. The total antioxidant activity of organic extracts of 37 algal samples, comprising of 30 species of Hawaiian algae from 27 different genera was determined. The activity was determined by employing the FRAP (Ferric Reducing Antioxidant Power) assays. Of the algae tested, the extract of Turbinaria ornata was found to be the most active. Bioassay-guided fractionation of this extract led to the isolation of a variety of different carotenoids as the active principles. The major bioactive antioxidant compound was identified as the carotenoid fucoxanthin. These results show, for the first time, that numerous Hawaiian algae exhibit significant antioxidant activity, a property that could lead to their application in one of many useful healthcare or related products as well as in chemoprevention of a variety of diseases including cancer.

  16. Bioactive compounds and antioxidant activity exhibit high intraspecific variability in Pleurotus ostreatus mushrooms and correlate well with cultivation performance parameters.

    Science.gov (United States)

    Koutrotsios, Georgios; Kalogeropoulos, Nick; Stathopoulos, Pantelis; Kaliora, Andriana C; Zervakis, Georgios I

    2017-05-01

    Experimental data related with oyster mushroom production and nutritional properties usually derive from the examination of only one strain, and hence their representativeness/usefulness is questionable. This work aims at assessing intraspecific variability in Pleurotus ostreatus by studying 16 strains, under the same conditions, in respect to essential cultivation and mushroom quality aspects, and by defining the impact of intrinsic/genetic factors on such parameters. Hence, mushroom yield, earliness, crop length, biological efficiency, productivity, and their content in selected macro and microconstituents (e.g. fatty acids, sterols, individual phenolic compounds, terpenic acids, glucans) as well as their antioxidant properties (i.e., antiradical activity, ferric reducing potential, inhibition of serum oxidation) were assayed. The effect of intrinsic/genetic factors was evident, especially as regards earliness, yield of each production flush and mushroom weight, whereas biological efficiency was not particularly influenced by the cultivated strain. Moreover, phenolics, ergosterol and antiradical activity demonstrated significant variability among strains in contrast to what was observed for fatty acids, β-glucans and ferric reducing potential. The observed heterogeneity reveals the limitations of using a low number of strains for evaluating mushroom production and/or their content in bioactive compounds, and as evidenced, it is valuable for breeding and commercial purposes.

  17. ANTIOXIDANT ACTIVITY OF BUTEA MONOSPERMA LEAF EXTRACTS

    Directory of Open Access Journals (Sweden)

    Shah Darshan

    2012-04-01

    Full Text Available Antioxidants are substances which help to defend the body against cell damage caused by various free radicals. Free radicals are unstable oxygen molecules containing unpaired electrons. Reactive oxygen species, such as superoxide anion, hydroxyl radical, and hydrogen peroxide, have a causal relationship with oxidative stress.Role of free radicals has been implicated in several diseases such as liver cirrhosis, atherosclerosis, Cancer, aging, arthritis, diabetes etc. the aim of the study was to investigate the antioxidant activity of Butea monosperma. The quantities of the Butea monosperma aqueous and benzene extracts needed for in vitro inhibition of hydroxyl radicals and lipid peroxidation were relatively similar to the known antioxidant ascorbic acid.

  18. Alkaloids with antioxidant activities from Aconitum handelianum.

    Science.gov (United States)

    Yin, Tian-Peng; Cai, Le; Xing, Yun; Yu, Jing; Li, Xue-Jiao; Mei, Rui-Feng; Ding, Zhong-Tao

    2016-06-01

    A new C20-diterpenoid alkaloid handelidine (1) and twenty-seven known alkaloids (2-28) were isolated from the roots of Aconitum handelianum. Their structures were established on the basis of extensive spectroscopic analyses. The study indicated that denudatine-type C20-diterpenoid alkaloids with vicinal-triol system and benzyltetrahydroisoquinoline alkaloids exhibited significant antioxidant activities measured by three antioxidant test systems. The aconitine-type C19-diterpenoid alkaloids could serve as potential secondary antioxidants for their strong binding effects to metal ions.

  19. High frequency in vitro plantlet regeneration and antioxidant activity ofEnicostema axillare (Lam.)Raynal ssp. littoralis (Blume)Raynal:An important medicinal plant

    Institute of Scientific and Technical Information of China (English)

    Kousalya Loganathan; VNarmatha Bai

    2014-01-01

    Objective:To develop a method for high frequency plantlets regeneration protocol forEnicostema axillare(Lam.)Raynal ssp.littoralis(Blume)Raynal(E. axillare) without intermediate callus phase and to determine the content and correlation of phenolic compounds and their antioxidant activity of both the plants derived from nature and nodal culture byDPPH assay.Methods:The nodal explants were cultured onMS basal medium fortified with different concentration of various growth regulators such asBAP,KIN,TDZ and2iP(0.5-2 mg/L) individually and in combinations with or withoutGA3 for shoot bud induction and multiplication.Total phenol and flavonoid content was determined in both plants from nature and nodal culture and antioxidant activity was determined byDPPH assay.Results:The highest number of multiple shoot(108.00±3.55 shoots/explants) was obtained onMS medium supplemented withBAP(2 mg/L) in combination withKIN (0.5 mg/L) andGA3(2 mg/L).Rooting was optimized on half-strengthMS medium supplemented withIAA(0.5 mg/L).The rooted plantlets were transferred to paper cups containing vermiculite and hardening was successfully attained with75% survival.Among the four extract of methanol and water extract from both the plantlet from nature and nodal culture, the concentration of flavonoid was found to be higher in methanol extract of the plants from nature(146.57±1.68 mg rutin/g extract) and phenol content was higher in the water extract of plant from nature(52.53±1.67 mgGAE /g extract).The radical scavenging activity of four extracts.Methanol extract of plant grown in nature showed the highest radical scavenging activity(IC50 =87.10 μg/mL) was investigated byDPPH test.Conculsion:The present study not only enables reinforcement of wild plant populations usingex situ growth of individuals, but it also helps for high scale production of plantlets.A high correlation between antioxidant capacities and their total phenolic contents indicated that flavonoid compounds were a major

  20. Antioxidant activity of Induna apple pomace extract

    Directory of Open Access Journals (Sweden)

    Savatović Slađana M.

    2005-01-01

    Full Text Available Different concentrations ofmethanol aqueous solution with or without 0.5% acetic acid and 80% acetone were used to achieve the highest yield of extraction of phenolics from Induna apple pomace. The highest content of phenolics (6.38 mg/g was detected in the 80% methanol extract. The influence of 80% methanol extract of Induna apple pomace on stable l,l-diphenyl-2-picrylhydrazyl (DPPH and reactive hydroxyl radicals has been investigated by electron spin resonance (ESR spectroscopy. Based on the obtained results it can be concluded that the investigated extract is more effective in the DPPH test than on the DMPO-OH scavenging. In both cases antioxidant activity increased with increasing concentration of the investigated extract. The high contents ofphenolics (6.38 mg/g, flavonoids (1.01 mg/g and flavan-3-ols (0.70 mg/g in 80% methanol extract indicated that these compounds contributed to the antioxidant activity of Induna apple pomace.

  1. In vitro antioxidant activities of antioxidant-enriched toothpastes.

    Science.gov (United States)

    Battino, M; Ferreiro, M S; Armeni, T; Politi, A; Bompadre, S; Massoli, A; Bullon, P

    2005-03-01

    Several forms of periodontal diseases (PD) are often associated with modified phagocytosing leukocytes and contemporary free radical production. Host antioxidant defenses could benefit from toothpastes used as adjuncts to counteract plaque-associated bacteria. The aim of the present study was to determine possible antioxidant activity (AA) of 12 differently antioxidant-enriched toothpastes, regardless of their efficacy as antimicrobial agents. Toothpastes were enriched alternatively with sodium ascorbyl phosphate, alpha-tocopherol acetate, pycnogenol, allantoin and methyl salycilate or a mixture of these. AA was tested in a cell-free system with a ABTS-decolorization assay improved by means of a flow injection analysis device. Comet assay, using NCTC 2544 keratinocytes, was performed to test if it was possible to identify any protection against in vitro DNA fragmentation provoked by a challenge with H(2)O(2) in cultures pre-incubated with toothpaste extracts. Only toothpastes containing sodium ascorbyl phosphate displayed clear AA with I(50) values ranging between 50 and 80 mg of toothpaste/ml water. COMET analysis of cells challenged with H(2)O(2) in presence of toothpaste extracts revealed a limited protection exerted by sodium ascorbyl phosphate. The results described herein indicate that toothpastes containing sodium ascorbyl phosphate possess AA. All the data were obtained in systems in vitro and the demonstration of in vivo AA is desirable. These findings could be useful in the treatment and maintenance of some forms of PD and should be considered when arranging new toothpaste formulations.

  2. In vitro antioxidant activities of Asteraceae Plants.

    Science.gov (United States)

    Vijaylakshmi, S; Nanjan, M J; Suresh, B

    2009-10-01

    Anaphalis neelgerriana DC and Cnicus wallichi DC belonging to the family Asteraceae (Compositae) are important medicinal plants indigenous to Nilgiris. Since the related species Anaphalis morrisonicola and Cnicus benedictus were reported for its anti cancer activities, the above mentioned plants were screened for Invitro antioxidant activity. In vitro antioxidant studies were carried out by DPPH, Nitric oxide and Hydrogen peroxide methods for the aerial part extracts of the plants. Different extracts were obtained from the aerial parts of the whole plant by successive solvent extraction and cold maceration process and subjected for Invitro antioxidant activity studies. Among the extracts tested, the ethyl acetate extract of Anaphalis neelgerriana DC and Cnicus wallchi DC showed significant anti oxidant activity in all the above methods. The potent ethyl acetate extract should be tested for anti oxidant activity in animal models.

  3. The expression and activity of antioxidant enzymes in the liver of rats exposed to high-fructose diet in the period from weaning to adulthood.

    Science.gov (United States)

    Glban, Alhadi M; Vasiljević, Ana; Veličković, Nataša; Nikolić-Kokić, Aleksandra; Blagojević, Duško; Matić, Gordana; Nestorov, Jelena

    2015-08-30

    Increased fructose consumption correlates with rising prevalence of various metabolic disorders, some of which were linked to oxidative stress. The relationship between fructose consumption and oxidative stress is complex and effects of a fructose-rich diet on the young population have not been fully elucidated. The aim of this study was to investigate whether high-fructose diet applied in the period from weaning to adulthood induces oxidative stress in the liver, thus contributing to induction or aggravation of metabolic disturbances in later adulthood. To that end we examined the effects of high-fructose diet on expression and activity of antioxidant enzymes, markers of lipid peroxidation and protein damage in the liver as the main fructose metabolizing tissue. High-fructose diet increased only SOD2 (mitochondrial manganese superoxide dismutase) activity, with no effect on other antioxidant enzymes, lipid peroxidation or accumulation of damaged proteins in the liver. The results show that fructose-induced metabolic disturbances could not be attributed to oxidative stress, at least not at young age. The absence of oxidative stress in the liver observed herein implies that young organisms are capable of maintaining redox homeostasis when challenged by fructose-derived energy overload. © 2014 Society of Chemical Industry.

  4. Determining antioxidant activities of lactobacilli cell-free supernatants by cellular antioxidant assay: a comparison with traditional methods.

    Directory of Open Access Journals (Sweden)

    Jiali Xing

    Full Text Available Antioxidant activity of lactic acid bacteria is associated with multiple health-protective effects. Traditional indexes of chemical antioxidant activities poorly reflect the antioxidant effects of these bacteria in vivo. Cellular antioxidant activity (CAA assay was used in this study to determine the antioxidant activity of cell-free supernatants (CFSs of 10 Lactobacillus strains. The performance of the CAA assay was compared with that of four chemical antioxidant activity assays, namely, DPPH radical scavenging, hydroxyl radical scavenging (HRS, reducing power (RP, and inhibition of linoleic acid peroxidation (ILAP. Results of the CAA assay were associated with those of DPPH and ILAP assays, but not with those of RP and HRS assays. The inter- and intra-specific antioxidant activities of CFS were characterized by chemical and CAA assays. L. rhamnosus CCFM 1107 displayed a high antioxidative effect similar to positive control L. rhamnosus GG ATCC 53103 in all of the assays. The CAA assay is a potential method for the detection of antioxidant activities of lactobacilli CFSs.

  5. Antioxidant and Antiradical Activity of Coffee

    Directory of Open Access Journals (Sweden)

    Alexander Yashin

    2013-10-01

    Full Text Available This review summarizes published information concerning the determination of antioxidant activity (AA in coffee samples by various methods (ORAC, FRAP, TRAP, TEAC, etc. in vitro and limited data of antiradical activity of coffee products in vitro and in vivo. Comparison is carried out of the AA of coffee Arabica and coffee Robusta roasted at different temperatures as well as by different roasting methods (microwave, convection, etc.. Data on the antiradical activity of coffee is provided. The antioxidant activity of coffee, tea, cocoa, and red wine is compared. At the end of this review, the total antioxidant content (TAC of coffee samples from 21 coffee-producing countries as measured by an amperometric method is provided. The TAC of green and roasted coffee beans is also compared.

  6. Antioxidant and Antiradical Activity of Coffee.

    Science.gov (United States)

    Yashin, Alexander; Yashin, Yakov; Wang, Jing Yuan; Nemzer, Boris

    2013-10-15

    This review summarizes published information concerning the determination of antioxidant activity (AA) in coffee samples by various methods (ORAC, FRAP, TRAP, TEAC, etc.) in vitro and limited data of antiradical activity of coffee products in vitro and in vivo. Comparison is carried out of the AA of coffee Arabica and coffee Robusta roasted at different temperatures as well as by different roasting methods (microwave, convection, etc.). Data on the antiradical activity of coffee is provided. The antioxidant activity of coffee, tea, cocoa, and red wine is compared. At the end of this review, the total antioxidant content (TAC) of coffee samples from 21 coffee-producing countries as measured by an amperometric method is provided. The TAC of green and roasted coffee beans is also compared.

  7. Multiple heat priming enhances thermo-tolerance to a later high temperature stress via improving subcellular antioxidant activities in wheat seedlings

    DEFF Research Database (Denmark)

    Wang, Xiao; Cai, Jian; Liu, Fulai

    2014-01-01

    Seedlings of winter wheat (Triticum aestivum L.) were firstly twice heat-primed at 32/24 °C, and subsequently subjected to a more severe high temperature stress at 35/27 °C. The later high temperature stress significantly decreased plant biomass and leaf total soluble sugars concentration. However...... an improvement of light use efficiency due to the priming pre-treatment. Under the later high temperature stress, PH could be maintained a better redox homeostasis than NH, as exemplified by the higher activities of superoxide dismutase (SOD) in chloroplasts and glutathione reductase (GR), and of peroxidase (POD......, heat priming effectively improved thermo-tolerance of wheat seedlings subjected to a later high temperature stress, which could be largely ascribed to the enhanced anti-oxidation at the subcellular level....

  8. Antioxidant activity relationship of phenolic compounds in Hypericum perforatum L.

    Directory of Open Access Journals (Sweden)

    Petrović Slobodan S

    2011-06-01

    Full Text Available Abstract Background The St John's Wort (Hypericum perforatum; Clusiaceae has been used in traditional and modern medicine for a long time due to its high content of biologically active phenolics. The purpose of this work was to develop a method for their fractionation and identification, and to determine the most active antioxidant compounds in plant extract. Results An LC-MS method which enables fast qualitative and semiquantitative analysis was developed. The composition determined is in agreement with the previous results, where 6 flavonoids, 4 naphthodianthrones and 4 phloroglucinols have been identified. Significant antioxidant activity was determined for most of the fractions by DPPH assay (the lowest IC50 of 0.52 μg/ml, NO scavenging (6.11 μg/ml, superoxide scavenging (1.86 μg/ml, lipid peroxidation (0.0079 μg/ml and FRAP (the highest reduction capacity of 104 mg Fe equivalents/g assays. Conclusion LC-MS technique has been successfully applied for a quick separation and identification of the major components of H. perforatum fractions. Majority of the fractions analyzed have expressed a very high antioxidative activity when compared to synthetic antioxidants. The antioxidant activity could be attributed to flavonoids and phenolic acids, while phloroglucinols and naphthodianthrones showed no significant activity. It is demonstrated that it is possible to obtain, by fractionation, H. perforatum preparations with significantly increased phloroglucinols-to-naphthodianthrones ratio (up to 95:5.

  9. Profiling Environmental Chemicals for Activity in the Antioxidant Response Element Signaling Pathway Using a High-Throughput Screening Approach

    Science.gov (United States)

    1 ABSTRACT 2 3 BACKGROUND: Oxidative stress has been implicated in the pathogenesis of a variety 4 of diseases ranging from cancer to neurodegeneration, highlighti.ng the need to identify 5 chemicals that can induce this effect. The antioxidant response element (ARE)...

  10. The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity.

    Science.gov (United States)

    Checovich, Mariana L; Galatro, Andrea; Moriconi, Jorge I; Simontacchi, Marcela; Dubcovsky, Jorge; Santa-María, Guillermo E

    2016-07-01

    TaNAM transcription factors play an important role in controlling senescence, which in turn, influences the delivery of nitrogen, iron and other elements to the grain of wheat (Triticum aestivum) plants, thus contributing to grain nutritional value. While lack or diminished expression of TaNAMs determines a stay-green phenotype, the precise effect of these factors on chloroplast structure has not been studied. In this work we focused on the events undergone by chloroplasts in two wheat lines having either control or diminished TaNAM expression due to RNA interference (RNAi). It was found that in RNAi plants maintenance of chlorophyll levels and maximal photochemical efficiency of photosystem II were associated with lack of chloroplast dismantling. Flow cytometer studies and electron microscope analysis showed that RNAi plants conserved organelle ultrastructure and complexity. It was also found that senescence in control plants was accompanied by a low leaf enzymatic antioxidant activity. Lack of chloroplast dismantling in RNAi plants was associated with maintenance of protein and iron concentration in the flag leaf, the opposite being observed in control plants. These data provide a structural basis for the observation that down regulation of TaNAMs confers a functional stay-green phenotype and indicate that the low export of iron and nitrogen from the flag leaf of these plants is concomitant, within the developmental window studied, with lack of chloroplast degradation and high enzymatic antioxidant activity.

  11. Antioxidative activities and active compounds of extracts from Catalpa plant leaves.

    Science.gov (United States)

    Xu, Hongyu; Hu, Gege; Dong, Juane; Wei, Qin; Shao, Hongbo; Lei, Ming

    2014-01-01

    In order to screen the Catalpa plant with high antioxidant activity and confirm the corresponding active fractions from Catalpa ovata G. Don, C. fargesii Bur., and C. bungei C. A. Mey., total flavonoid contents and antioxidant activities of the extracts/fractions of Catalpa plant leaves were determined. The determined total flavonoid content and antioxidant activity were used as assessment criteria. Those compounds with antioxidant activity were isolated with silica gel column chromatography and ODS column chromatography. Our results showed that the total flavonoid content in C. bungei C. A. Mey. (30.07 mg/g · DW) was the highest, followed by those in C. fargesii Bur. (25.55 mg/g · DW) and C. ovata G. Don (24.96 mg/g · DW). According to the determination results of total flavonoid content and antioxidant activity in 3 clones of leaves of C. bungei C. A. Mey., the total flavonoid content and antioxidant activity in crude extracts from C. bungei C. A. Mey. 6 (CA6) leaves were the highest. Moreover, the results showed that the total flavonoid content and antioxidant activities of ethyl acetate (EA) fraction in ethanol crude extracts in CA6 leaves were the highest, followed by n-butanol, petroleum ether (PE), and water fractions. Two flavonoid compounds with antioxidant activity were firstly isolated based on EA fraction. The two compounds were luteolin (1) and apigenin (2), respectively.

  12. Antioxidative Activities and Active Compounds of Extracts from Catalpa Plant Leaves

    Directory of Open Access Journals (Sweden)

    Hongyu Xu

    2014-01-01

    Full Text Available In order to screen the Catalpa plant with high antioxidant activity and confirm the corresponding active fractions from Catalpa ovata G. Don, C. fargesii Bur., and C. bungei C. A. Mey., total flavonoid contents and antioxidant activities of the extracts/fractions of Catalpa plant leaves were determined. The determined total flavonoid content and antioxidant activity were used as assessment criteria. Those compounds with antioxidant activity were isolated with silica gel column chromatography and ODS column chromatography. Our results showed that the total flavonoid content in C. bungei C. A. Mey. (30.07 mg/g·DW was the highest, followed by those in C. fargesii Bur. (25.55 mg/g·DW and C. ovata G. Don (24.96 mg/g·DW. According to the determination results of total flavonoid content and antioxidant activity in 3 clones of leaves of C. bungei C. A. Mey., the total flavonoid content and antioxidant activity in crude extracts from C. bungei C. A. Mey. 6 (CA6 leaves were the highest. Moreover, the results showed that the total flavonoid content and antioxidant activities of ethyl acetate (EA fraction in ethanol crude extracts in CA6 leaves were the highest, followed by n-butanol, petroleum ether (PE, and water fractions. Two flavonoid compounds with antioxidant activity were firstly isolated based on EA fraction. The two compounds were luteolin (1 and apigenin (2, respectively.

  13. Antioxidant Activity in Extracts of 27 Indigenous Taiwanese Vegetables

    Directory of Open Access Journals (Sweden)

    Pi-Yu Chao

    2014-05-01

    Full Text Available The objectives of this study were to identify the antioxidants and antioxidant axtivity in 27 of Taiwan’s indigenous vegetables. Lycium chinense (Lc, Lactuca indica (Li, and Perilla ocymoides (Po contained abundant quercetin (Que, while Artemisia lactiflora (Al and Gynura bicolor (Gb were rich in morin and kaempferol, respectively. Additionally, Nymphoides cristata (Nc and Sechium edule (Se-yellow had significantly higher levels of myricetin (Myr than other tested samples. Cyanidin (Cyan and malvidin (Mal were abundant in Gb, Abelmoschus esculentus Moench (Abe, Po, Anisogonium esculentum (Retz. Presl (Ane, Ipomoea batatas (Ib-purple, and Hemerocallis fulva (Hf-bright orange. Relatively high levels of Trolox equivalent antioxidant capacity (TEAC, oxygen radical absorption capacity (ORAC, and 1,1-diphenyl-2-picryl-hydrazyl (DPPH radical scavenger were generated from extracts of Toona sinensis (Ts and Po. Significant and positive correlations between antioxidant activity and polyphenols, anthocyanidins, Que, Myr, and morin were observed, indicating that these phytochemicals were some of the main components responsible for the antioxidant activity of tested plants. The much higher antioxidant activity of Po, Ts, and Ib (purple leaf may be related to their higher Cyan, Que, and polyphenol content.

  14. Phenolic Extracts from Acacia mangium Bark and Their Antioxidant Activities

    Directory of Open Access Journals (Sweden)

    Liangliang Zhang

    2010-05-01

    Full Text Available Phenolic compounds are present at very high concentrations in the bark of Acacia mangium. These compounds are known to have strong antioxidant activity and thus different beneficial effects on human health. Phenolic compounds in bark of A. mangium were extracted and their antioxidant activities were investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH free radical-scavenging and ferric-reducing antioxidant power (FRAP assays. A central composite design has been employed to optimize the experimental conditions for a high total phenolic content and antioxidant activity. The desirability function approach has been employed to simultaneously optimize the three responses: total phenols, antiradical activity and FRAP. An extraction time of 90 min, liquid-solid ratio of 5, and temperature of 50 °C was predicted for the optimum experimental conditions using the desirability function. A significant linear relationship between antioxidant potency, antiradical activity and the content of phenolic compounds of bark extracts was observed. The structures of condensed tannins isolated from A. mangium were characterized by MALDI-TOF MS analyses. Condensed tannin oligomers from A. mangium were shown to be heterogeneous mixtures consisting of procyanidin and prodelphinidin structural units with polymerization degrees up to 9.

  15. Phenolic extracts from Acacia mangium bark and their antioxidant activities.

    Science.gov (United States)

    Zhang, Liangliang; Chen, Jiahong; Wang, Yongmei; Wu, Dongmei; Xu, Man

    2010-05-14

    Phenolic compounds are present at very high concentrations in the bark of Acacia mangium. These compounds are known to have strong antioxidant activity and thus different beneficial effects on human health. Phenolic compounds in bark of A. mangium were extracted and their antioxidant activities were investigated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging and ferric-reducing antioxidant power (FRAP) assays. A central composite design has been employed to optimize the experimental conditions for a high total phenolic content and antioxidant activity. The desirability function approach has been employed to simultaneously optimize the three responses: total phenols, antiradical activity and FRAP. An extraction time of 90 min, liquid-solid ratio of 5, and temperature of 50 degrees C was predicted for the optimum experimental conditions using the desirability function. A significant linear relationship between antioxidant potency, antiradical activity and the content of phenolic compounds of bark extracts was observed. The structures of condensed tannins isolated from A. mangium were characterized by MALDI-TOF MS analyses. Condensed tannin oligomers from A. mangium were shown to be heterogeneous mixtures consisting of procyanidin and prodelphinidin structural units with polymerization degrees up to 9.

  16. Nanostructured amorphous nickel oxide with enhanced antioxidant activity

    Energy Technology Data Exchange (ETDEWEB)

    Madhu, G. [Department of Physics, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala 695581 (India); Department of Physics, University College, Thiruvananthapuram, Kerala 695034 (India); Biju, V., E-mail: bijunano@gmail.com [Department of Physics, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala 695581 (India)

    2015-07-15

    Highlights: • Synthesis of nanostructured amorphous nickel oxide by a facile chemical route. • Enhanced antioxidant activity of amorphous NiO compared to crystalline samples. • Role of O{sup 2−} vacancies and high specific surface area in antioxidant activity. • Use of DC conductivity, XPS and BET to explain enhanced antioxidant activity. - Abstract: Nanostructured amorphous nickel oxide was synthesized by the thermal decomposition of nickel chloride–ethanol amine complex. The X-ray diffraction and Transmission Electron Microscopic studies established the amorphous nature of the sample. The Fourier Transform Infrared, Scanning Electron Microscopy, Energy Dispersive and X-ray Photoelectron Spectroscopic studies of the sample revealed the formation of NiO. The specific surface area of the sample is measured using Brunauer–Emmett–Teller analysis and the mesoporous nature of the sample is established through Barrett–Joyner–Halenda pore size distribution analysis. The antioxidant activity of the amorphous sample measured by 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging is found to be nearly twice greater than that reported for nanocrystalline NiO samples. The estimated radical scavenging activity of the sample is correlated with the DC conductivity values measured in vacuum and air ambience. The enhanced antioxidant activity of the amorphous NiO is accounted by the increase in the concentration of O{sup 2−} vacancies and the specific surface area. The Ni 2p and O 1s X-ray Photoelectron Spectroscopic studies of the sample support the inference.

  17. Multiple heat priming enhances thermo-tolerance to a later high temperature stress via improving subcellular antioxidant activities in wheat seedlings.

    Science.gov (United States)

    Wang, Xiao; Cai, Jian; Liu, Fulai; Dai, Tingbo; Cao, Weixing; Wollenweber, Bernd; Jiang, Dong

    2014-01-01

    Seedlings of winter wheat (Triticum aestivum L.) were firstly twice heat-primed at 32/24 °C, and subsequently subjected to a more severe high temperature stress at 35/27 °C. The later high temperature stress significantly decreased plant biomass and leaf total soluble sugars concentration. However, plants experienced priming (PH) up-regulated the Rubisco activase B encoding gene RcaB, which was in accordance with the higher photosynthesis rate in relation to the non-primed plants (NH) under the later high temperature stress. In relation to NH, the major chlorophyll a/b-binding protein gene Cab was down-regulated in PH plants, implying a reduction of the light absorption to protect the photosystem II from excitation energy under high temperature stress. At the same time, under the later high temperature stress PH plants showed significantly higher actual photochemical efficiency, indicating an improvement of light use efficiency due to the priming pre-treatment. Under the later high temperature stress, PH could be maintained a better redox homeostasis than NH, as exemplified by the higher activities of superoxide dismutase (SOD) in chloroplasts and glutathione reductase (GR), and of peroxidase (POD) in mitochondria, which contributed to the lower superoxide radical production rate and malondialdehyde concentration in both chloroplasts and mitochondria. The improved antioxidant capacity in chloroplasts and mitochondria was related to the up-regulated expressions of Cu/Zn-SOD, Mn-SOD and GR in PH. Collectively, heat priming effectively improved thermo-tolerance of wheat seedlings subjected to a later high temperature stress, which could be largely ascribed to the enhanced anti-oxidation at the subcellular level.

  18. Antioxidant Activities of Nine Selected Culinary Spices from China

    Institute of Scientific and Technical Information of China (English)

    Liang Ying; Ding Ying; Zhang Liu-juan; Liu Xian-jin

    2015-01-01

    The antioxidant activities and the total phenolic contents of the water and/or ethanol extracts of the nine selected culinary spices from China were systematically investigated. Both ethanol extracts and water extracts had high ability of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, 2, 2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging and ferric reducing antioxidant power (FRAP). The ethanol extract of Sichuan pepper showed the highest ability of DPPH radical scavenging. The extract with the highest ABTS radical scavenging effect was that of ethanol extract of cinnamon. Both ethanol and water extracts of cinnamon possessed the high ferric reducing antioxidant power (FRAP) with the values of 4 541.87 and 1 134.52 µmol of Fe (Ⅱ)/g. The extracts with high hydroxyl radical-mediated deoxyribose degradation were all the ethanol extracts as follows:cinnamon, bay leaf, Sichuan pepper, star anise and fennel. The extracts with high antioxidant activities also had the high contents of the phenols. The study indicated that these spices might be potentially be used as natural antioxidants in food.

  19. Antioxidant activity of puha (Sonchus oleraceus L.) as assessed by the cellular antioxidant activity (CAA) assay.

    Science.gov (United States)

    McDowell, Arlene; Thompson, Scott; Stark, Mirjam; Ou, Zong-Quan; Gould, Kevin S

    2011-12-01

    There is considerable interest in antioxidant dietary components that can be protective against degenerative diseases in humans. Puha (Sonchus oleraceus L.) is a rich source of polyphenols, and exhibits strong antioxidant activity as measured by the 2,2-diphenylpicrylhydrazyl (DPPH) assay. However, the potential of puha to protect against degenerative diseases requires that low molecular weight antioxidants (LMWA) are absorbed by, and active in, human cells. The cellular antioxidant activity (CAA) assay was used to investigate the antioxidant activity of puha leaf extracts. Preparation methods of freezing and freeze-drying reduced the total polyphenolic content compared with fresh puha, but did not affect the LMWA potential as determined by the DPPH assay. The IC(50) values were 0.012 ± 0.003 mg/mL and 0.010 ± 0.005 mg/mL for freeze-dried and fresh puha leaves, respectively. Using the CAA assay, it was shown that LMWAs from foliar extracts of puha were effectively absorbed into HepG2 cells, and exerted antioxidant activity at levels comparable to those of extracts from blueberry fruits, the much-touted antioxidant superfood. Methylene blue staining of HepG2 cells indicated that puha extracts were not cytotoxic at concentrations below 100 mg DW/mL. The data indicate the potential of puha as a nutraceutical supplement for human health.

  20. Antioxidant activities and total phenol content of Inula viscosa extracts selected from three regions of Morocco

    Directory of Open Access Journals (Sweden)

    Naima Chahmi

    2015-03-01

    Conclusions: Our results of antioxidant assays were justified and partially supported the popular usage of the tested plants. The high antioxidant activity found in the plant from Sefrou and its great biomass in this region suggested that Inula viscosa is a good source of natural antioxidants compounds which might have benefits for health.

  1. Metal concentration and antioxidant activity of edible mushrooms from Turkey.

    Science.gov (United States)

    Sarikurkcu, Cengiz; Tepe, Bektas; Kocak, Mehmet Sefa; Uren, Mehmet Cemil

    2015-05-15

    This study presents information on the antioxidant activity and heavy metal concentrations of Polyporus sulphureus, Macrolepiota procera, Lycoperdon perlatum and Gomphus clavatus mushrooms collected from the province of Mugla in the South-Aegean Region of Turkey. Antioxidant activities of mushroom samples were evaluated by four complementary tests. All tests showed L. perlatum and G. clavatus to possess extremely high antioxidant potential. Antioxidant activity of the samples was strongly correlated with total phenolic-flavonoid content. In terms of heavy metal content, L. perlatum exceeded the legal limits for daily intake of Pb, Fe, Mn, Cr, Ni and Co contents (0.461, 738.00, 14.52, 1.27, 1.65, 0.417 mg/day, respectively) by a 60-kg consumer. Co contents of M. procera (0.026 mg/day) and P. sulphureus (0.030 mg/day) and Cd contents of G. clavatus (0.071 mg/day) were also above the legal limits. According to these results, L. perlatum should not be consumed, despite the potentially beneficial antioxidant activity. Additionally, M. procera and G. clavatus should not be consumed daily due to their high levels of Cd and Co.

  2. SYNTHESIS AND ANTIOXIDANT ACTIVITY OF TWO ISOESPINTANOL

    Directory of Open Access Journals (Sweden)

    Paula Galeano

    2011-01-01

    Full Text Available The antioxidant activity of isoespintanol (1 hemisynthetic analogues, 4-bromo-2- isopropyl-3,6-dimethoxy-5-methylphenol (2 and 3-isopropyl-6-methylbenzene- 1,2,4-triol (3, was evaluated using ABTS, DPPH and FRAP assays. Partial rationalization of the results is provided in terms of quantum chemical calculations of bond dissociation enthalpy (BDE and ionization potential (IP.

  3. Antioxidant Activity of Ecdysteroids from Serratula strangulata

    Institute of Scientific and Technical Information of China (English)

    DAI,Jing-Qiu(戴静秋); CAI,Yu-Jun(蔡育军); SHI,Yan-Ping(师彦平); ZHANG,Yong-Hong(张永红); LIU,Zhong-Li(刘中立); YANG,Li(杨立); LI,Yu(李瑜)

    2002-01-01

    One new ecdysteroid, (24R)-24-(2-hydroxyethyl)-20-hydroxyecdysone (3), as well as three known ecdysteroids, has been isolated from Chinese herb Serratula strangulata and these compounds 1-4 showed effective antioxidant activity on AAPH-induced hemolysis of human RBC and Fe2+ + cysteine-induced lipid peroxidation of liver microsome.

  4. Antioxidant activity of Moringa oleifera tissue extracts.

    Science.gov (United States)

    Santos, Andréa F S; Argolo, Adriana C C; Paiva, Patrícia M G; Coelho, Luana C B B

    2012-09-01

    Moringa oleifera is an important source of antioxidants, tools in nutritional biochemistry that could be beneficial for human health; the leaves and flowers are used by the population with great nutritional importance. This work investigates the antioxidant activity of M. oleifera ethanolic (E1) and saline (E2) extracts from flowers (a), inflorescence rachis (b), seeds (c), leaf tissue (d), leaf rachis (e) and fundamental tissues of stem (f). The radical scavenging capacity (RSC) of extracts was determined using dot-blots on thin layer chromatography stained with a 0.4 mM 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) solution; spectrophotometric assays were recorded (515 nm). Antioxidant components were detected in all E1 and E2 from a, b and d. The best RSC was obtained with E1d; the antioxidants present in E2 reacted very slowly with DPPH. The chromatogram revealed by diphenylborinate-2-ethylamine methanolic solution showed that the ethanolic extract from the flowers, inflorescence rachis, fundamental tissue of stem and leaf tissue contained at least three flavonoids; the saline extract from the flowers and leaf tissue revealed at least two flavonoids. In conclusion, M. oleifera ethanolic and saline extracts contain antioxidants that support the use of the plant tissues as food sources.

  5. Antioxidant enzymes activities in obese Tunisian children

    Directory of Open Access Journals (Sweden)

    Sfar Sonia

    2013-01-01

    Full Text Available Abstract Background The oxidant stress, expected to increase in obese adults, has an important role in the pathogenesis of many diseases. It results when free radical formation is greatly increased or protective antioxidant mechanisms are compromised. The main objective of this study is to evaluate the antioxidant response to obesity-related stress in healthy children. Methods A hundred and six healthy children (54 obese and 52 controls, aged 6–12 years old, participated in this study. The collected data included anthropometric measures, blood pressure, fasting glucose, total cholesterol, triglycerides and enzymatic antioxidants (Superoxide dismutase: SOD, Catalase: CAT and Glutathione peroxidase: GPx. Results The first step antioxidant response, estimated by the SOD activity, was significantly higher in obese children compared with normal-weight controls (p  Conclusions The obesity-related increase of the oxidant stress can be observed even in the childhood period. In addition to the complications of an increased BMI, obesity itself can be considered as an independent risk factor of free radical production resulting in an increased antioxidant response.

  6. The antioxidant activity of selected wild orchids of Nepal

    Directory of Open Access Journals (Sweden)

    Mukesh Babu Chand

    2016-09-01

    Full Text Available Objective: To assess the antioxidant activity as well as the total polyphenolics and flavonoids content of thirteen extracts from nine wild orchids of Nepal. Methods: First, a preliminary phytochemical screening of thirteen extracts of nine orchids was carried out using established protocols. Then, the total polyphenolic and flavonoid contents of each extract were evaluated using the Folin-Ciocalteu's phenol reagent and aluminium chloride methods respectively. Finally, antioxidant activity was determined by 2, 2-diphenyl-1-picryl hydrazyl free radical scavenging assay and expressed in terms of half inhibition concentrations (IC50 values. Results: All the tested parameters showed significant variation at P = 0.05. The total flavonoids varied with the greatest amounts found in the leaves of Rhynchostylis retusa and the smallest amounts found in the roots of Gastrochilus acutifolius (G. acutifolius. The amount of total polyphenolics was highest in the stems of Vanda cristata and lowest in the leaves of G. acutifolius. Antioxidant activity was highest in the stems of Vanda cristata and lowest in the leaves of G. acutifolius. Conclusions: Some of the orchid extracts studied exhibited levels of antioxidant activity which were comparable to or even higher than those found for extracts of medicinal plants and orchids studied previously. It is suggested that drugs and natural food additives can be extracted from those wild orchids with high levels of antioxidant activity.

  7. Antioxidant activity of wheat and buckwheat flours

    Directory of Open Access Journals (Sweden)

    Sedej Ivana J.

    2010-01-01

    Full Text Available Antioxidative activities of wheat flours (type 500 and wholegrain and buckwheat flours (light and wholegrain were tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH·-scavenging activity, reducing power and chelating activity on Fe2+. Also, the content of the total phenolics of ethanolic extracts was estimated. Polyphenolics content (expressed as gallic acid equivalent, GAE in wheat flours varied between 37.1 and 137.2 μg GAE/g extract, while its content in buckwheat flour were at least four time higher and ranged between 476.3 and 618.9μg GAE/g extract. Ethanolic extracts of buckwheat flours exhibited higher antioxidant activities in all the assays, except for chelating activity. Regarding all the obtained results, it can be concluded that bakery products produced with buckwheat flour could be regarded as potential functional foods.

  8. Antioxidant activity of hydrophilic and lipophilic extracts of Brazilian blueberries.

    Science.gov (United States)

    Pertuzatti, Paula Becker; Barcia, Milene Teixeira; Rodrigues, Daniele; da Cruz, Pollyanna Nogueira; Hermosín-Gutiérrez, Isidro; Smith, Robert; Godoy, Helena Teixeira

    2014-12-01

    Hydrophilic and lipophilic extracts of ten cultivars of Highbush and Rabbiteye Brazilian blueberries (Vaccinium corymbosum L. and Vacciniumashei Reade, respectively) that are used for commercial production were analysed for antioxidant activity by the FRAP, ORAC, ABTS and β-carotene-linoleate methods. Results were correlated to the amounts of carotenoids, total phenolics and anthocyanins. Brazilian blueberries had relatively high concentration of total phenolics (1,622-3,457 mg gallic acid equivalents per 100 g DW) and total anthocyanins (140-318 mg cyanidin-3-glucoside equivalents per 100 g DW), as well as being a good source of carotenoids. There was a higher positive correlation between the amounts of these compounds and the antioxidant activity of hydrophilic compared to lipophilic extracts. There were also significant differences in the level of bioactive compounds and antioxidant activities between different cultivars, production location and year of cultivation. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Phytochemical profile, toxicity and antioxidant activity of Aloysia gratissima (Verbenaceae

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    Ana Lúcia B. Zeni

    2013-01-01

    Full Text Available Aloysia gratissima (Gill. et Hook Tronc. (Verbenaceae is native to South America with folk therapeutic applications for a wide range of diseases. The polyphenolic and carotenoid profile, toxicity, and antioxidant activity of aqueous extract of Aloysia gratissima were investigated. HPLC analyses showed high amounts of ferulic acid, trans-cinnamic acid and p-coumaric acid, and also trans-β- carotene and lutein which fluctuated throughout the seasons. Furthermore, the extract investigated not only exerted antioxidant activity but also inhibited lipid peroxidation. Toxicity was achieved only at the highest dose tested. Therefore, A. gratissima is a potential species for medicinal purposes.

  10. Antioxidant and Antiacetylcholinesterase Activity of Teucrium hyrcanicum

    Directory of Open Access Journals (Sweden)

    Fereshteh Golfakhrabadi

    2015-01-01

    Full Text Available Background: Teucrium hyrcanicum belonging to the Lamiaceae family is a native plant in Iran; it is called Maryam nokhodi-e-jangali in Farsi. Objective: The aim of this study is to evaluate acetylcholinesterase inhibition (AChEI, antioxidant activity and flavonoids content of T. hyrcanicum methanol extract. Materials and Methods: The air-dried and the ground aerial parts of T. hyrcanicum were extracted by percolation method with methanol. Antioxidant activity of the extract was investigated by using 2,2′-diphenyl-1-picrylhydrazyl (DPPH and ferric reducing antioxidant power assay (FRAP methods. In addition, AChEI and flavonoid content of T. hyrcanicum methanol extract were measured. Results: The results showed that total flavonoid content of T. hyrcanicum in reference to the standard curve for quercetin was 20.70 ± 0.05 mg quercetin equivalents/g of extract. In the FRAP method, the antioxidant activity of T. hyrcanicum extract and butyl hydroxyanisole (BHA (as a positive control were 657.5 ± 0.04 and 880 ± 0.06 mmol Fe II/1 g dried extract. According to results of DPPH assay, half maximal inhibitory concentration (IC 50 value for DPPH radical-scavenging activities of T. hyrcanicum methanol extract, vitamin E and BHA were 74.6, 14.12 and 7.8 μg/mL, respectively. IC 50 value for AChEI of T. hyrcanicum and donepezil as a positive control were 2.12 mg/mL and 0.013 mg/mL. Conclusion: The results of the present study showed T. hyrcanicum is a natural antioxidant that the flavonoid content can be responsible for extract effects.

  11. Determination of fourteen polyphenols in pulses by high performance liquid chromatography-diode array detection (HPLC-DAD) and correlation study with antioxidant activity and colour.

    Science.gov (United States)

    Giusti, Federica; Caprioli, Giovanni; Ricciutelli, Massimo; Vittori, Sauro; Sagratini, Gianni

    2017-04-15

    Pulses, which include lentils, beans, chickpeas, peas, and soybeans, provide an important source of proteins, dietary fibers, minerals and vitamins, as well as such important bioactive molecules as polyphenols. The presence of polyphenols is often related to the colour of the pulse and to its antioxidant activity. The aim of this work was to set up a new HPLC-DAD method for simultaneously analysing 14 polyphenolic compounds, including two anthocyanins, in different varieties of pulses and to correlate the polyphenol content with the seed coat colour and the antioxidant activity. The total content of the analysed polyphenols ranged from 3mg/kg for dehulled red lentils to 1630.5mg/kg for ruviotto beans. Samples with dark testa (or seed coat), namely black lentils and diavoli beans, had higher antioxidant activity than those with pale testa, and a positive correlation was found between total phenolic content (TPC) and IC50 for dark coloured varieties.

  12. Fruit antioxidant activity, ascorbic acid, total phenol, quercetin, and carotene of Irwin mango fruits stored at low temperature after high electric field pretreatment.

    Science.gov (United States)

    Shivashankara, K S; Isobe, Seiichiro; Al-Haq, Muhammad Imran; Takenaka, Makiko; Shiina, Takeo

    2004-03-10

    Greenhouse-grown tree ripe (TR) and mature green (MG) mangoes (cv. Irwin) were exposed to high electric field treatment before 20 and 30 days of storage at 5 degrees C. MG fruits were allowed to ripen at room temperature after low-temperature storage. Fruit physical quality attributes, ascorbic acid, carotene, quercetin, total phenols, and antioxidant capacity were estimated before and after the storage period. Antioxidant capacity of fruit juice was estimated using the ferric reducing antioxidant power (FRAP) assay. Fruit firmness decreased significantly during storage. Titratable acidity decreased 20 days after storage. Total soluble solids did not change during storage. Antioxidant capacity of fruits remained unchanged up to 20 days of storage period and decreased thereafter. Total phenol and carotenes increased during storage. Antioxidant capacity of fruits was significantly correlated only to ascorbic acids. Peel color and carotenes were higher in TR fruits, whereas titratable acidity and firmness were higher in MG fruits. There was no significant difference in other parameters between the stages of picking. Electric field pretreatment affected the respiration and antioxidant capacity of TR fruits and did not have any significant affect on other parameters. TR mangoes of cv. Irwin are more suitable for low-temperature storage and can be successfully stored for up to 20 days at 5 degrees C without any significant losses in functional properties and quality attributes.

  13. In vitro antioxidant and antihyperlipidemic activities of Bauhinia variegata Linn

    Directory of Open Access Journals (Sweden)

    Rajani G

    2009-01-01

    Full Text Available Objectives : To evaluate the ethanolic and aqueous extracts of Bauhinia variegata Linn. for in vitro antioxidant and antihyperlipidemic activity. Materials and Methods : Ethanolic and aqueous extracts of the stem bark and root of B. variegata Linn. were prepared and assessed for in vitro antioxidant activity by various methods namely total reducing power, scavenging of various free radicals such as 1,2-diphenyl-2-picrylhydrazyl (DPPH, super oxide, nitric oxide, and hydrogen peroxide. The percentage scavenging of various free radicals were compared with standard antioxidants such as ascorbic acid and butylated hydroxyl anisole (BHA. The extracts were also evaluated for antihyperlipidemic activity in Triton WR-1339 (iso-octyl polyoxyethylene phenol-induced hyperlipidemic albino rats by estimating serum triglyceride, very low density lipids (VLDL, cholesterol, low-density lipids (LDL, and high-density lipid (HDL levels. Result : Significant antioxidant activity was observed in all the methods, (P < 0.01 for reducing power and (P < 0.001 for scavenging DPPH, super oxide, nitric oxide, and hydrogen peroxide radicals. The extracts showed significant reduction (P < 0.01 in cholesterol at 6 and 24 h and (P < 0.05 at 48 h. There was significant reduction (P < 0.01 in triglyceride level at 6, 24, and 48 h. The VLDL level was also significantly (P < 0.05 reduced from 24 h and maximum reduction (P < 0.01 was seen at 48 h. There was significant increase (P < 0.01 in HDL at 6, 24, and 48 h. Conclusion : From the results, it is evident that alcoholic and aqueous extracts of B. variegata Linn. can effectively decrease plasma cholesterol, triglyceride, LDL, and VLDL and increase plasma HDL levels. In addition, the alcoholic and aqueous extracts have shown significant antioxidant activity. By the virtue of its antioxidant activity, B. variegata Linn. may show antihyperlipidemic activity.

  14. Antioxidant activities in extracts of selected indigenous vegetables ...

    African Journals Online (AJOL)

    SAM

    2014-04-23

    Apr 23, 2014 ... anion radicals and reducing power) than ethanol extracts. ... species (or their active constituents) identified as having high levels of antioxidant ... carbonate solution was added to the mixture and adjusted to 10 ml with distilled ... aqueous ethanol and to the test tubes 0.1 ml of 10% aluminium nitrate was ...

  15. Evaluation of antioxidant activities of Hippophae rhamnoides Linn leaves extracts

    Institute of Scientific and Technical Information of China (English)

    JavidAli; BashirAhmad

    2015-01-01

    Objective: To investigate the antioxidant activity of aqueous, methanol, ethanol, acetone, ethyl acetate, chloroform and n-hexane extracts of Hippophae rhamnoides (H. rhamnoides) leaves. Methods: Antioxidant activity was evaluated by using in-vitro antioxidant assays model 1, 1’-diphenyl-2-picrylhydrazyl radical-scavenging activity. The antioxidant activities were compared with standard antioxidant agents such as ascorbic acid. Results: The antioxidant activities (% inhibition) of all the tested extracts were increased in the order i.e. menthol > ethanol > aqueous > acetone > chloroform > ethyl acetate > n-hexane. The methanol extract EC50 (µg/mL) value was compatible with vitamin C (standard). The antioxidant activity of H. rhamnoides leaves extracts increased in a dose dependent manner. Conclusions: It was observed that H. rhamnoides was a potential resource of antioxidants and thus could put off numerous radical linked diseases.

  16. Flavanol content and antioxidant activity in winery byproducts.

    Science.gov (United States)

    González-Paramás, Ana M; Esteban-Ruano, Sara; Santos-Buelga, Celestino; de Pascual-Teresa, Sonia; Rivas-Gonzalo, Julián C

    2004-01-28

    Proanthocyanidins, particularly those coming from wine and grape products, have became of interest to nutritionists. Particular attention is currently being paid to the exploitation of this kind of grape byproducts for obtaining bio-active phenolic compounds with potential application as food antioxidants and preventive agents against cancer and other diseases. In this work, the flavanol composition of various winery byproducts submitted to different degrees of industrial exploitation has been studied and their antioxidant activity determined using two different methods (TBARS and TEAC) to evaluate their interest as suitable sources for the preparation of flavanol-rich antioxidant extracts. All the byproducts studied were still good flavanol sources no matter their exploitation degree. An important conclusion was that dried grape seeds, obtained as an end byproduct after the color extraction and alcohol distillation of the wine pomace, still kept important flavanol concentrations and significant antioxidant activity, even if they were submitted to high temperatures. These byproducts can be considered a cheap source for the extraction of antioxidant flavanols, which can be used as dietary supplements or in the production of phytochemicals.

  17. In vitro synergistic antioxidant activity and identification of antioxidant components from Astragalus membranaceus and Paeonia lactiflora.

    Directory of Open Access Journals (Sweden)

    Xiaoyan Xu

    Full Text Available Many traditionally used herbs demonstrate significantly better pharmacological effects when used in combination than when used alone. However, the mechanism underlying this synergism is still poorly understood. This study aimed to investigate the synergistic antioxidant activity of Astragalus membranaceus (AME and Paeonia Lactiflora (PL, and identify the potential antioxidant components by 1,1-diphenyl-2-picrylhydrazine (DPPH radical spiking test followed by a high performance liquid chromatography separation combined with diode array detection and tandem mass spectrometry analysis (DPPH-HPLC-DAD-MS/MS. Eight AME-PL combined extracts (E1-E8 were prepared based on bioactivity-guided fractionation. Among them, E1 exhibited the strongest synergistic effect in scavenging DPPH radicals and reducing ferric ions (P<0.05. Moreover, E1 presented strong cytoprotection against H2O2-induced oxidative damage in MRC-5 cells by suppressing the decrease of the superoxide dismutase (SOD, glutathione peroxidase (GSH-Px and catalase (CAT activities. A strong correlation between the increment of total phenolic/flavonoid and synergistic antioxidant activity, especially between the increment of total flavonoid and the increase in ferric reducing power was observed. Finally, seven antioxidant substances were identified in E1 as oxypaeoniflora, catechin, calycosin-7-O-β-D-glucopyranoside, fomononetin-7-O-β-D-glucopyranoside, 9,10-dimethoxy-pterocarpan-3-O-β-D-glucopyranoside, quercetin and 2'-dihydroxy-3',4'-dimethyl-isoflavan-7-O-β-D-glucopyranoside.

  18. Antioxidant and antiproliferative activities of common fruits.

    Science.gov (United States)

    Sun, Jie; Chu, Yi-Fang; Wu, Xianzhong; Liu, Rui Hai

    2002-12-01

    Consumption of fruits and vegetables has been associated with reduced risk of chronic diseases such as cardiovascular disease and cancer. Phytochemicals, especially phenolics, in fruits and vegetables are suggested to be the major bioactive compounds for the health benefits. However, the phenolic contents and their antioxidant activities in fruits and vegetables were underestimated in the literature, because bound phenolics were not included. This study was designed to investigate the profiles of total phenolics, including both soluble free and bound forms in common fruits, by applying solvent extraction, base digestion, and solid-phase extraction methods. Cranberry had the highest total phenolic content, followed by apple, red grape, strawberry, pineapple, banana, peach, lemon, orange, pear, and grapefruit. Total antioxidant activity was measured using the TOSC assay. Cranberry had the highest total antioxidant activity (177.0 +/- 4.3 micromol of vitamin C equiv/g of fruit), followed by apple, red grape, strawberry, peach, lemon, pear, banana, orange, grapefruit, and pineapple. Antiproliferation activities were also studied in vitro using HepG(2) human liver-cancer cells, and cranberry showed the highest inhibitory effect with an EC(50) of 14.5 +/- 0.5 mg/mL, followed by lemon, apple, strawberry, red grape, banana, grapefruit, and peach. A bioactivity index (BI) for dietary cancer prevention is proposed to provide a new alternative biomarker for future epidemiological studies in dietary cancer prevention and health promotion.

  19. Antioxidant activity of polyphenols in carob pods.

    Science.gov (United States)

    Kumazawa, Shigenori; Taniguchi, Masa; Suzuki, Yasuyuki; Shimura, Masayo; Kwon, Mi-Sun; Nakayama, Tsutomu

    2002-01-16

    We extracted polyphenols from carob (Ceratonia siliqua L.) pods, and evaluated the in vitro antioxidant activity of the crude polyphenol fraction (CPP). The total polyphenol content in CPP determined by the Folin-Ciocalteu method was 19.2%. The condensed tannin content determined by the vanillin and proanthocyanidin assay systems was 4.37% and 1.36%, respectively. beta-Carotene bleaching, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, inhibition of lipid peroxidation by the erythrocyte ghost, and microsomal assay systems were used to evaluate the antioxidant activity. CPP showed a stronger inhibitory effect against the discoloration of beta-carotene than other polyphenol compounds such as catechins and procyanidins. CPP had weaker antioxidant activity in the DPPH free radical scavenging, the erythrocyte ghost, and microsomal systems than authentic polyphenol compounds at the same concentrations. The activity adjusted by the polyphenol concentration was, however, comparable to that of authentic polyphenol compounds. Considering most carob pods are discarded and not effectively utilized at present, these results suggested that carob pods could be utilized as a functional food or food ingredient.

  20. Hop pellets as an interesting source of antioxidant active compounds

    Directory of Open Access Journals (Sweden)

    Andrea Holubková

    2013-02-01

    Full Text Available Hop is a plant used by humankind for thousands of years. This plant is one of the main and indispensable raw materials for the beer production. It is used for various dishes preparation in the cuisine. Hop is also used to inhibit bacterial contamination. The hop extracts are used for its sedative, antiseptic and antioxidant properties in medicine, as a part of many phytopharmaceuticals. The present paper have focused on the extraction of polyphenolic compounds from 4 samples of hop pellets varieties of Aurora, Saaz, Lublin and Saphir, on the analyzing of bioactive substances (polyphenolics and flavonoids in prepared extracts and on the determination of antioxidant activity.  The highest content of polyphenolic substances was determined in the sample Lublin (153.06 mg gallic acid (GAE/g and Saaz (151.87 mg GAE/g. The amount of flavonoids in the samples  was descending order Saaz > Saphir > Aurora > Lublin. Hops, as plant, is known by high content of antioxidant active substances. Antioxidant activity was determined using three independent spectrofotometric methods, radical scavenging assays using 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS and 1,1-diphenyl-2-picrylhydrazyl (DPPH radical and ferric reducing antioxidant power (FRAP. The sample Aurora showed the highest ability to scavenge of ABTS radical cation. Antioxidant activity continued to decline in a row Saphir> Lublin> Saaz. The same trend was also observed by using the FRAP assay. The most effective DPPH radical scavengering activity had the sample Saaz a Saphir (p>0.05.doi:10.5219/270 Normal 0 21 false false false SK X-NONE X-NONE

  1. STRUCTURE – ANTIOXIDANT ACTIVITIES RELATIONSHIP ANALYSIS OF ISOEUGENOL, EUGENOL, VANILIN AND THEIR DERIVATIVES

    Directory of Open Access Journals (Sweden)

    Nur Aini

    2010-06-01

    Full Text Available Structure Activity Relationship (SAR technique between the theoretical parameters and antioxidant activities of isoeugenol, eugenol, vanillin and their derivatives as Mannich reaction products, have been analyzed. Antioxidant activities were examined by oxidation reaction of oleic acid at 60 °C with b-carotene methods, whereas theoretical parameters of the activities were determined by calculating Bonding Dissociation Enthalpy (BDE and net charge of oxygen atom(-OH using AM1 semi empiric methods. The result from both test showed in the following orders: BHT > Mannich product of isoeugenol > isoeugenol > Mannich product of eugenol > eugenol > Mannich product of vanillin > vanillin. The antioxidant activities increase with small the BDE value and high the net charge. Electron donating groups will increase the antioxidants activity with lowering the BDE value and increasing the net charge, while electron-withdrawing groups will decrease antioxidants activity.   Keywords: SAR, antioxidants, Bonding Dissociation Entalphy, eugenol.

  2. Antioxidant activity of cod (Gadus morhua) protein hydrolysates: Fractionation and characterisation of peptide fractions

    DEFF Research Database (Denmark)

    Farvin Habebullah, Sabeena; Andersen, Lisa Lystbæk; Otte, Jeanette;

    2016-01-01

    This study aimed to characterise peptide fractions (>5 kDa, 3–5 kDa and antioxidative activity obtained from a cod protein hydrolysate. The free amino acids in all fractions were dominated by Ala, Gly, Glu and Ser. The total amino acid composition had high proportions of Lys, Ala...... to the antioxidative activity of the peptide fractions, and Tyr seemed to play a major role in the antioxidant activity....

  3. Chemical Composition and Antioxidant Activity of Euterpe oleracea Roots and Leaflets

    OpenAIRE

    2016-01-01

    Euterpe oleracea (açaí) is a palm tree well known for the high antioxidant activity of its berries used as dietary supplements. Little is known about the biological activity and the composition of its vegetative organs. The objective of this study was to investigate the antioxidant activity of root and leaflet extracts of Euterpe oleracea (E. oleracea) and characterize their phytochemicals. E. oleracea roots and leaflets extracts were screened in different chemical antioxidant assays (DPPH—2,...

  4. Antioxidant Activity in Two Pearl Millet (Pennisetum typhoideum Cultivars as Influenced by Processing

    Directory of Open Access Journals (Sweden)

    Florence Suma Pushparaj

    2014-02-01

    Full Text Available Research on the effect of processing on the retention of bioactive components with potential antioxidant activity is gaining importance. The objective of this investigation was to evaluate the effect of various processing methods (milling, boiling, pressure cooking, roasting and germination respectively on the antioxidant components as well as the antioxidant activities in the commonly used pearl millet cultivars—Kalukombu (K and Maharashtra Rabi Bajra (MRB. The methanolic extracts of processed pearl millet flours were analyzed for 1,1-diphenyl-2-picrylhydrazyl (DPPH free radical scavenging activity, reducing power assay (RPA and ferric reducing antioxidant power (FRAP assays respectively. The samples were also evaluated for tannin, phytic acid and flavonoid content which was then correlated with the antioxidant activity assayed using three methods. The results indicated that the bran rich fraction showed high antioxidant activity (RPA owing to high tannin, phytic acid and flavonoid levels. Heat treatments exhibited significantly (P ≤ 0.05 higher antioxidant activity (DPPH scavenging activity and RPA reflecting the high flavonoid content. Processing did not have any significant effect on the FRAP activity of pearl millet. The data on the correlation coefficient suggests that DPPH radical scavenging activity and reducing power assay in the K variety was largely due to the presence of flavonoid content, however in MRB, no relationship was found between antioxidant activities and antioxidant components.

  5. Antioxidants in Raspberry: On-line analysis links antioxidant activity to a diversity of individual metabolites

    NARCIS (Netherlands)

    Beekwilder, M.J.; Jonker, H.H.; Hall, R.D.; Meer, van der I.M.; Vos, de C.H.

    2005-01-01

    The presence of antioxidant compounds can be considered as a quality parameter for edible fruit. In this paper, we studied the antioxidant compounds in raspberry (Rubus idaeus) fruits by high-performance liquid chromatography (HPLC) coupled to an on-line postcolumn antioxidant detection system. Both

  6. distribution of nutrients, polyphenols and antioxidant activities in the ...

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    KEY WORDS: Antioxidant, Mushrooms, Nutrients, Pilei, Polyphenols, Stipes. INTRODUCTION ... importance to vegetarians. Edible mushrooms .... Natural polyphenols exert their beneficial health effects by their antioxidant activity ... The basis for the lack of agreement between phenolic distribution and reducing power of.

  7. Antioxidant Activities of Methanol Extract and Solvent Fractions of ...

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    Tropical Journal of Pharmaceutical Research March 2015; 14 (3): 503-509 ... Original Research Article. Antioxidant Activities of ... to be used as alternatives to synthetic antioxidants ..... verifying their bioactivity in cellular and animal models.

  8. Antioxidant and anti-inflammatory activities of selected Chinese medicinal plants and their relation with antioxidant content

    Directory of Open Access Journals (Sweden)

    Ravipati Anjaneya S

    2012-10-01

    Full Text Available Abstract Background The main aim of this study is to evaluate the antioxidant and anti-inflammatory properties of forty four traditional Chinese medicinal herbal extracts and to examine these activities in relation to their antioxidant content. Methods The antioxidant activities were investigated using DPPH radical scavenging method and yeast model. The anti-inflammatory properties of the herbal extracts were evaluated by measuring their ability to inhibit the production of nitric oxide and TNF-α in RAW 264.7 macrophages activated by LPS and IFN- γ, respectively. The cytotoxic effects of the herbal extracts were determined by Alomar Blue assay by measuring cell viability. In order to understand the variation of antioxidant activities of herbal extracts with their antioxidant contents, the total phenolics, total flavonoids and trace metal (Mg, Mn, Cu, Zn, Se and Mo quantities were estimated and a correlation analysis was carried out. Results Results of this study show that significant levels of phenolics, flavonoids and trace metal contents were found in Ligustrum lucidum, Paeonia suffuticosa, Salvia miltiorrhiza, Sanguisorba officinalis, Spatholobus suberectus, Tussilago farfara and Uncaria rhyncophylla, which correlated well with their antioxidant and anti-inflammatory activities. Some of the plants displayed high antioxidant and anti-inflammatory activities but contained low levels of phenolics and flavonoids. Interestingly, these plants contained significant levels of trace metals (such as Zn, Mg and Se which are likely to be responsible for their activities. Conclusions The results indicate that the phenolics, flavonoids and trace metals play an important role in the antioxidant activities of medicinal plants. Many of the plants studied here have been identified as potential sources of new antioxidant compounds.

  9. Comparison of antioxidant activities of different parts from snow chrysanthemum (Coreopsis tinctoria Nutt.) and identification of their natural antioxidants using high performance liquid chromatography coupled with diode array detection and mass spectrometry and 2,2'-azinobis(3-ethylbenzthiazoline-sulfonic acid)diammonium salt-based assay.

    Science.gov (United States)

    Chen, L X; Hu, D J; Lam, S C; Ge, L; Wu, D; Zhao, J; Long, Z R; Yang, W J; Fan, B; Li, S P

    2016-01-08

    Snow chrysanthemum (Coreopsis tinctoria Nutt.), a world-widely well-known flower tea material, has attracted more and more attention because of its beneficial health effects such as antioxidant activity and special flavor. In this study, a high performance liquid chromatography coupled with diode array detection and mass spectrometry (HPLC-DAD-MS) and 2,2'-azinobis(3-ethylbenzthiazoline-sulfonic acid)diammonium salt (ABTS) based assay was employed for comparison and identification of antioxidants in different samples of snow chrysanthemum. The results showed that snow chrysanthemum flowers possessed the highest while stems presented the lowest antioxidant capacities. Fourteen detected peaks with antioxidant activity were temporarily identified as 3,4',5,6,7-pentahydroxyflavanone-O-hexoside, chlorogenic acid, 2R-3',4',8-trihydroxyflavanone-7-O-glucoside, flavanomarein, flavanocorepsin, flavanokanin, quercetagitin-7-O-glucoside, 3',5,5',7-tetrahydroxyflavanone-O-hexoside, marein, maritimein, 1,3-dicaffeoylquinic acid, coreopsin, okanin and acetyl-marein by comparing their UV spectra, retention times and MS data with standards or literature data. Antioxidants existed in snow chrysanthemum are quite different from those reported in Chrysanthemum morifolium, a well-known traditional beverage in China, which indicated that snow chrysanthemum may be a promising herbal tea material with obvious antioxidant activity.

  10. Antioxidant Contents and Antioxidant Activities of White and Colored Potatoes (Solanum tuberosum L.).

    Science.gov (United States)

    Lee, Sang Hoon; Oh, Seung Hee; Hwang, In Guk; Kim, Hyun Young; Woo, Koan Sik; Woo, Shun Hee; Kim, Hong Sig; Lee, Junsoo; Jeong, Heon Sang

    2016-06-01

    This study was performed to evaluate and compare the antioxidant substance content and antioxidant activities of white (Superior) and colored (Hongyoung, Jayoung, Jasim, Seohong, and Jaseo) potatoes. The potatoes were extracted with 80% ethanol and were evaluated for the total polyphenol, flavonoid, and anthocyanin contents and for 1,1-diphenyl- 2-picrylhydrazyl (DPPH)/2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity, reducing power, and ferrous metal ion chelating effect. The total polyphenol, flavonoid, and anthocyanin contents of Hongyoung and Jayoung were higher than white and other colored potatoes. All colored potato extracts, except for Jaseo and Seohong, showed higher ABTS radical scavenging activities than the general white potato extract. Hongyoung and Jayoung had the highest ABTS and DPPH radical scavenging activities. Optical density values for the reducing power of Jayoung and Jaseo at concentration of 2 mg/mL were 0.148 and 0.090, respectively. All colored potato extracts had lower ferrous metal ion chelating effect than the white potato. A significant (P<0.05) positive correlation was observed between total polyphenol content and total flavonoid content (r=0.919), anthocyanin content (r=0.992), and ABTS radical scavenging activity (r=0.897). Based on these results, this research may be useful in developing the Hongyoung and Jayoung cultivars with high antioxidant activities.

  11. In vitro antioxidant activity of Barleria noctiflora L. f.

    Institute of Scientific and Technical Information of China (English)

    Sangilimuthu Alagar Yadav; Anitha Jabamalai Raj; R Sathishkumar

    2012-01-01

    Objective: To evaluate the antioxidant activity of defatted methanol extract of Barleria noctiflora (B. noctiflora) L.f. leaf and root using in vitro models. Methods: DPPH radical scavenging activity, ferrous reducing power, Fe2+ chelating activity assay, nitric oxide radical scavenging activity ABTS.+ radical cation decolourisation assay, superoxide anion and hydrogen peroxide radical scavenging activities were studied. Results: The extracts showed good antioxidant capacity in DPPH radical scavenging assay, when compared to other in vitro models and the IC50 value were found to be 150 μg/mL in leaf extract and 140 μg/mL in root extract. The total phenolic content using Folin's-Ciocalteu reagent indicated that 1 mg of leaf and root extracts contain 368 μg and 481 μg with gallic acid equivalent and also the total flavonoid content found to be 240 μg and 410μg respectively with quercetin equivalence. Conclusion: The results showed that the antioxidant potential of the extracts is high in root extract compared to the leaf extract. This is the first ever report of antioxidant studies in B. noctiflora L. f.

  12. Antioxidant activity of selected wild Canadian prairie fruits

    Directory of Open Access Journals (Sweden)

    Dorota Klensporf-Pawlik

    2015-12-01

    Full Text Available Background. Canadian prairies are a habitat for unique wild plants. The main object of the present study was to investigate phytochemicals content and antioxidant activity in seven wild Canadian prairie fruits. Material and methods. The presence of total phenolics, flavonoids, anthocyanins and antioxidant activity were identified in the extracts according to standard procedure. Results. Wild rose had the highest amounts of total phenolics and total flavonoids, whereas elderberry ex- hibited the highest amount of anthocyanins. All extracts showed good scavenging activities towards DPPH radicals. The results showed a good linear relationship between oxygen radical absorbance capacity and total phenolics indicating that radicals are scavenged at a greater rate as the total phenolics content increases. Addi- tionally, all extracts when applied at concentration of 800 ppm, showed ability to inhibit oxidation of canola oil. In SOT test the best results were obtained when extract of American mountain ash was used. In general, wild rose followed by American mountain ash demonstrated the highest antioxidant activity among assessed Canadian prairie fruits. Conclusion. From the results it can be concluded that prairie fruit extracts are a rich source of phenolic compounds and poses a high antioxidant activity, confirmed by assessment with different type of radicals employed.

  13. Dietary antioxidant supplementation enhances lipid and protein oxidative stability of chicken broiler meat through promotion of antioxidant enzyme activity1

    Science.gov (United States)

    Delles, Rebecca M.; Xiong, Youling L.; True, Alma D.; Ao, Touying; Dawson, Karl A.

    2014-01-01

    Recent nutrigenomic studies have shown that animal nutrition can have a major influence on tissue gene expression. Dietary antioxidant supplements can enhance the quality of meat through modification of tissue metabolic processes. This study investigated the influence of dietary antioxidants and quality of oil on the oxidative and enzymatic properties of chicken broiler breast meat stored in an oxygen-enriched package (HiOx: 80% O2/20% CO2) in comparison with air-permeable polyvinylchloride (PVC) or skin packaging systems during retail display at 2 to 4°C for up to 21 d. Broilers were fed either a diet with a low-oxidized (peroxide value 23 mEq of O2/kg) or high-oxidized (peroxide value 121 mEq of O2/kg) oil, supplemented with or without an algae-based Se yeast and organic mineral antioxidant pack for 42 d. Lipid and protein oxidation and tissue enzymatic activity were analyzed. In all packaging systems, lipid oxidation (TBA reactive substances) was inhibited by up to 32.5% (P antioxidant-supplemented diet when compared with diets without antioxidants, particularly in the HiOx and PVC systems. Protein sulfhydryls were significantly protected by antioxidant diets (e.g., by 14.6 and 17.8% for low-and high-oxidized dietary groups, respectively, in PVC d 7 samples). Glutathione peroxidase, catalase, and superoxide dismutase activities were significantly higher (P antioxidant-supplemented diets compared with the basal diet, regardless of oil quality. Also, serum carbonyls were lower in broilers fed a low-oxidized antioxidant-supplemented treatment. The results demonstrate that dietary antioxidants can minimize the oxidative instability of proteins and lipids, and the protection may be linked to improved cellular antioxidant enzymatic activity. PMID:24879706

  14. Dietary antioxidant supplementation enhances lipid and protein oxidative stability of chicken broiler meat through promotion of antioxidant enzyme activity.

    Science.gov (United States)

    Delles, Rebecca M; Xiong, Youling L; True, Alma D; Ao, Touying; Dawson, Karl A

    2014-06-01

    Recent nutrigenomic studies have shown that animal nutrition can have a major influence on tissue gene expression. Dietary antioxidant supplements can enhance the quality of meat through modification of tissue metabolic processes. This study investigated the influence of dietary antioxidants and quality of oil on the oxidative and enzymatic properties of chicken broiler breast meat stored in an oxygen-enriched package (HiOx: 80% O2/20% CO2) in comparison with air-permeable polyvinylchloride (PVC) or skin packaging systems during retail display at 2 to 4°C for up to 21 d. Broilers were fed either a diet with a low-oxidized (peroxide value 23 mEq of O2/kg) or high-oxidized (peroxide value 121 mEq of O2/kg) oil, supplemented with or without an algae-based Se yeast and organic mineral antioxidant pack for 42 d. Lipid and protein oxidation and tissue enzymatic activity were analyzed. In all packaging systems, lipid oxidation (TBA reactive substances) was inhibited by up to 32.5% (P antioxidant-supplemented diet when compared with diets without antioxidants, particularly in the HiOx and PVC systems. Protein sulfhydryls were significantly protected by antioxidant diets (e.g., by 14.6 and 17.8% for low-and high-oxidized dietary groups, respectively, in PVC d 7 samples). Glutathione peroxidase, catalase, and superoxide dismutase activities were significantly higher (P antioxidant-supplemented diets compared with the basal diet, regardless of oil quality. Also, serum carbonyls were lower in broilers fed a low-oxidized antioxidant-supplemented treatment. The results demonstrate that dietary antioxidants can minimize the oxidative instability of proteins and lipids, and the protection may be linked to improved cellular antioxidant enzymatic activity. Poultry Science Association Inc.

  15. Hydrophilic carbon clusters as therapeutic, high capacity antioxidants

    Science.gov (United States)

    Samuel, Errol L. G.; Duong, MyLinh T.; Bitner, Brittany R.; Marcano, Daniela C.; Tour, James M.; Kent, Thomas A.

    2014-01-01

    Oxidative stress reflects an excessive accumulation of reactive oxygen species (ROS) and is a hallmark of several acute and chronic human pathologies. While many antioxidants have been investigated, the majority have demonstrated poor efficacy in clinical trials. Here, we discuss limitations of current antioxidants and describe a new class of nanoparticle antioxidants, poly(ethylene glycol)-functionalized hydrophilic carbon clusters (PEG-HCCs). PEG-HCCs show high capacity to annihilate ROS such as superoxide and hydroxyl radicals, show no reactivity toward nitric oxide, and can be functionalized with targeting moieties without loss of activity. Given these properties, we propose that PEG-HCCs offer an exciting new area of study for treatment of numerous ROS-induced human pathologies. PMID:25175886

  16. [Antioxidant activity of cationic whey protein isolate].

    Science.gov (United States)

    titova, M E; Komolov, S A; Tikhomirova, N A

    2012-01-01

    The process of lipid peroxidation (LPO) in biological membranes of cells is carried out by free radical mechanism, a feature of which is the interaction of radicals with other molecules. In this work we investigated the antioxidant activity of cationic whey protein isolate, obtained by the cation-exchange chromatography on KM-cellulose from raw cow's milk, in vitro and in vivo. In biological liquids, which are milk, blood serum, fetal fluids, contains a complex of biologically active substances with a unique multifunctional properties, and which are carrying out a protective, antimicrobial, regenerating, antioxidant, immunomodulatory, regulatory and others functions. Contents of the isolate were determined electrophoretically and by its biological activity. Cationic whey protein isolate included lactoperoxidase, lactoferrin, pancreatic RNase, lysozyme and angeogenin. The given isolate significantly has an antioxidant effect in model experimental systems in vitro and therefore may be considered as a factor that can adjust the intensity of lipid oxidation. In model solutions products of lipid oxidation were obtained by oxidation of phosphatidylcholine by hydrogen peroxide in the presence of a source of iron. The composition of the reaction mixture: 0,4 mM H2O2; 50 mcM of hemin; 2 mg/ml L-alpha-phosphatidylcholine from soybean (Sigma, German). Lipid peroxidation products were formed during the incubation of the reaction mixture for two hours at 37 degrees C. In our studies rats in the adaptation period immediately after isolation from the nest obtained from food given orally native cationic whey protein isolate at the concentration three times higher than in fresh cow's milk. On the manifestation of the antioxidant activity of cationic whey protein isolate in vivo evidence decrease of lipid peroxidation products concentration in the blood of rats from the experimental group receipt whey protein isolate in dos 0,6 mg/g for more than 20% (pwhey protein isolate has an

  17. Antioxidant activity and kinetics studies of eugenol and 6-bromoeugenol.

    Science.gov (United States)

    Mahboub, Radia; Memmou, Faiza

    2015-01-01

    In this work, we report the antioxidant and free radical scavenging activity of 6-bromoeugenol and eugenol. EC50, the concentration providing 50% inhibition, is calculated and the antioxidant activity index (AAI) is evaluated. The antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging method. EC50 values of 6-bromoeugenol, ascorbic acid and eugenol were 34.270 μg/mL, 54.888 μg/mL and 130.485 μg/mL, respectively. 6-Bromoeugenol showed higher AAI value (1.122) followed by ascorbic acid (0.700), then by eugenol (0.295). We also investigate the kinetics of DPPH radical scavenging activity of our products to determine the useful parameter TEC50 to evaluate their antiradical efficiency (ARE). Our results have shown high ARE. This study has provided the following ARE ( × 10(-3)) order for the tested antioxidants: ascorbic acid (70.119)>6-bromoeugenol (34.842) > eugenol (21.313). Finally, we classify ascorbic acid and eugenol as fast kinetics reaction (TEC50 8.82 and 11.38 min, respectively) and 6-bromoeugenol as medium kinetics reaction (TEC50 39.24 min).

  18. Preparation and antioxidant activity of tyrosyl and homovanillyl ethers.

    Science.gov (United States)

    Madrona, A; Pereira-Caro, G; Bravo, L; Mateos, R; Espartero, J L

    2011-12-01

    Preparation of tyrosyl and homovanillyl lipophilic derivatives was carried out as a response to the food industry's increasing demand for new synthetic lipophilic antioxidants. Tyrosyl and homovanillyl ethers were synthesized in high yields by a three-step procedure starting from tyrosol (Ty) and homovanillic alcohol (HMV). The antioxidant activity of these new series of alkyl tyrosyl and homovanillyl ethers was evaluated by the Rancimat test in a lipophilic food matrix and by the FRAP, ABTS and ORAC assays and compared to free Ty and HMV as well as two antioxidants widely used in the food industry, butylhydroxytoluene (BHT) and α-tocopherol. The results pointed out the higher activity of homovanillyl series in comparison with tyrosyl series with all the assayed methods. However, while both synthetic series were less antioxidant than BHT and α-tocopherol in a lipophilic matrix after their Rancimat test evaluation, homovanillyl alkyl ethers showed the best reducing power and radical scavenging activity of all evaluated compounds. This batch of synthetic lipophilic compounds, derived from biologically active compounds such as Ty and HMV, provide interesting and potentially bioactive compounds.

  19. Phenolic Profiles and Antioxidant Activity of Lotus Root Varieties

    Directory of Open Access Journals (Sweden)

    Yang Yi

    2016-06-01

    Full Text Available Lotus root attracts increasing attention mainly because of its phenolic compounds known as natural antioxidants. Its thirteen varieties were systematically analyzed on the content, distribution, composition and antioxidant activity of phenolic compounds for a better understanding of this aquatic vegetable. The respective mean contents of total phenolics in their flesh, peel and nodes were 1.81, 4.30 and 7.35 mg gallic acid equivalents (GAE/g fresh weight (FW, and those of total flavonoids were 3.35, 7.69 and 15.58 mg rutin equivalents/g FW. The phenolic composition determined by a high-performance liquid chromatography method varied significantly among varieties and parts. The phenolics of flesh were mainly composed of gallocatechin and catechin; those of peel and node were mainly composed of gallocatechin, gallic acid, catechin and epicatechin. The antioxidant activities of phenolic extracts in increasing order were flesh, peel and node; their mean concentrations for 50% inhibition of 2,2-diphenyl-1-picrylhydrazyl radical were 46.00, 26.43 and 21.72 µg GAE/mL, and their mean values representing ferric reducing antioxidant power were 75.91, 87.66 and 100.43 µg Trolox equivalents/100 µg GAE, respectively. “Zoumayang”, “Baheou”, “No. 5 elian” and “Guixi Fuou” were the hierarchically clustered varieties with relatively higher phenolic content and stronger antioxidant activity as compared with the others. Especially, their nodes and peels are promising sources of antioxidants for human nutrition.

  20. Microwave-assisted efficient extraction of different parts of Hippophae rhamnoides for the comparative evaluation of antioxidant activity and quantification of its phenolic constituents by reverse-phase high-performance liquid chromatography (RP-HPLC).

    Science.gov (United States)

    Sharma, Upendra K; Sharma, Kapil; Sharma, Nandini; Sharma, Abhishek; Singh, Harsh P; Sinha, Arun K

    2008-01-23

    The outcome of different extraction procedures (microwave, ultrasound, Soxhlet, and maceration) on the antioxidant activity of seeds, leaves, pulp, and fruits of Hippophae rhamnoides (sea buckthorn or SBT) was investigated by two different bioassays: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The SBT extracts were found to possess strong antioxidant activity measured in terms of TEAC (2.03-182.13 and 6.97-282.75 mg/g) with ABTS and DPPH assays, respectively. In general, the antioxidant capacity of microwave-assisted extracts was found to be significantly higher than those obtained by ultrasound-assisted extraction (UAE) and maceration while being slightly higher than Soxhlet extracts. Further, microwave extracts of seeds were found to possess maximum antioxidant capacity followed by leaves, fruits, and pulp. Also, the chemical composition of extracts, studied in terms of the total phenolic content, was found to be in the range of 1.9-23.5 mg/g Gallic acid equivalent (GAE), which indicates a strong correlation between antioxidant activity and phenolic content present in the SBT. In addition, some of its bioactive phenolic constituents, such as rutin ( 1), quercetin-3- O-galactoside ( 2), quercetin ( 3), myricetin ( 4), kaempferol ( 5), and isorhamnetin ( 6), were also quantified in different extracts by reverse-phase high-performance liquid chromatography (RP-HPLC).

  1. Pterocarpus marsupium extract reveals strong in vitro antioxidant activity.

    Science.gov (United States)

    Mohammadi, M; Khole, S; Devasagayam, T Pa; Ghaskadbi, S S

    2009-08-01

    Diabetes mellitus is a complex chronic disease characterized by hyperglycemia, which via several mechanism leads to an increase in production of reactive oxygen species (ROS) leading to various secondary complications. Thus, a drug having both antidiabetic and antioxidant properties would have great therapeutic value for overcoming the oxidative load in diabetes. The present study was aimed at extensively evaluating the antioxidant properties of an anti-diabetic plant extract of stem bark of Pterocarpus marsupium using various in vitro radical scavenging assays as well as by using liver slice cultures as a model system. Our results demonstrate that the whole aqueous extract showed high antioxidant activity in all different assays used and also protected mitochondria against oxidative damage. Ethanol was used as an inducer of oxidative stress in liver slice culture and cytotoxicity was estimated by quantitating release of cytotoxicity marker enzymes such as lactate dehydrogenase (LDH). Additionally, levels of antioxidant enzymes (AOEs) namely superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase were also estimated. The whole aqueous extract significantly reduced LDH release along with reduction of lipid peroxidation compared to ethanol treated slices. These results indicate that the P. marsupium extract may serve as a potential source of natural antioxidant for treatment of diabetes.

  2. Antioxidant, antitubercular and cytotoxic activities of Piper imperiale.

    Science.gov (United States)

    Diaz, Luis E; Munoz, Diego R; Prieto, Rosa E; Cuervo, Sergio A; Gonzalez, Diego L; Guzman, Juan D; Bhakta, Sanjib

    2012-04-05

    Phenolic compounds are widely distributed in Nature and act as pharmacologically active constituents in many herbal medicines. They have multiple biological properties, most notably antioxidant, antibacterial and cytotoxic activities. In the present study an attempt to correlate the phenolic composition of leaf, flower and wood extracts of Piper imperiale, with antioxidant, antitubercular and cytotoxic activities was undertaken. The total phenol content ranged from 1.98 to 6.94 mg GAE/gDW among ethanolic extracts, and gallic acid, catechin, epicatechin, ferulic acid, resveratrol and quercetin were identified and quantified by HPLC. DPPH and ABTS assays showed high antioxidant activity of the leaf extract (EC(50ABTS) = 15.6 µg/mL, EC(50DPPH) = 27.3 µg/mL) with EC₅₀ in the same order of magnitude as the hydroxyquinone (EC(50ABTS) = 10.2 µg/mL, EC(50DPPH) = 15.7 µg/mL). The flower extract showed strong antimicrobial activity against Mycobacterium tuberculosis H₃₇Rv. All the extracts exhibited dose-dependent cytotoxic effects against MCF-7 cancer cells. This is the first time that a Piper extract has been found to be highly active against M. tuberculosis. This study shows the biological potential of Piper imperiale extracts and gives way to bio-guided studies with well-defined biological activities.

  3. Antioxidant, Antitubercular and Cytotoxic Activities of Piper imperiale

    Directory of Open Access Journals (Sweden)

    Sanjib Bhakta

    2012-04-01

    Full Text Available Phenolic compounds are widely distributed in Nature and act as pharmacologically active constituents in many herbal medicines. They have multiple biological properties, most notably antioxidant, antibacterial and cytotoxic activities. In the present study an attempt to correlate the phenolic composition of leaf, flower and wood extracts of Piper imperiale, with antioxidant, antitubercular and cytotoxic activities was undertaken. The total phenol content ranged from 1.98 to 6.94 mg GAE/gDW among ethanolic extracts, and gallic acid, catechin, epicatechin, ferulic acid, resveratrol and quercetin were identified and quantified by HPLC. DPPH and ABTS assays showed high antioxidant activity of the leaf extract (EC50ABTS = 15.6 µg/mL, EC50DPPH = 27.3 µg/mL with EC50 in the same order of magnitude as the hydroxyquinone (EC50ABTS = 10.2 µg/mL, EC50DPPH = 15.7 µg/mL. The flower extract showed strong antimicrobial activity against Mycobacterium tuberculosis H37Rv. All the extracts exhibited dose-dependent cytotoxic effects against MCF-7 cancer cells. This is the first time that a Piper extract has been found to be highly active against M. tuberculosis. This study shows the biological potential of Piper imperiale extracts and gives way to bio-guided studies with well-defined biological activities.

  4. IN VITRO ANTIOXIDANT ACTIVITY OF SAUROPUS BACCIFORMIS BLUME (EUPHORBIACEAE

    Directory of Open Access Journals (Sweden)

    Arockia Jenecius Alphonse

    2012-09-01

    Full Text Available The present study aims to evaluate the in vitro antioxidant activity of methanol extract of different parts of Sauropus bacciformis Blume. Among the investigated plant parts, highest amount of total phenolics and flavonoids were reported in the stem. The in vitro antioxidant activity was evaluated by DPPH radical scavenging activity, hydroxyl scavenging activity, superoxide scavenging activity and ABTS radical cation scavenging activity with reference standard ascorbic acid and trolox. The methanol extract showed highest in vitro antioxidant activities. These in vitro assays indicate that this plant extracts is a significant source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.

  5. Variation in major antioxidants and total antioxidant activity of Yuzu (Citrus junos Sieb ex Tanaka) during maturation and between cultivars.

    Science.gov (United States)

    Yoo, Kyung Mi; Lee, Ki Won; Park, Jae Bok; Lee, Hyong Joo; Hwang, In Kyeong

    2004-09-22

    Epidemiological studies suggest that a high consumption of fruits can reduce the risk of some cancers and cardiovascular disease, and this may be attributable to the antioxidant activity of vitamins and phenolic compounds. The present study investigated the variations in vitamin C, total phenolic, hesperidin, and naringin contents, and total antioxidant activity of yuzu (Citrus junos Sieb ex Tanaka)-which is a popular citrus fruit in Korea and Japan-between cultivars and during maturity. The amounts of phenolics and vitamin C and the antioxidant activity in all tested yuzu cultivars were higher in peel than in flesh. Ripening increased the total antioxidant activity and vitamin C content in both peel and flesh of yuzu. However, the amounts of all total phenolics, hesperidin, and naringin in peel increased with ripening, whereas they decreased slightly in flesh. There was a highly linear relationship between the vitamin C content and the total antioxidant activity in both peel (r(2) = 1.000) and flesh (r(2) =0.998), suggesting that vitamin C plays a key role in the antioxidant activity of yuzu. In addition, the contribution of each antioxidant to the total antioxidant activity of yuzu was determined using a 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay and is expressed here in terms of the vitamin C equivalent antioxidant capacity (VCEAC). The means of vitamin C, naringin, and hesperidin in yuzu were 90.4, 63.8, and 65.7 mg/100 g fresh yuzu, respectively. The relative VCEAC values of these compounds were in the following order: vitamin C (1.00) > naringin (0.195) > hesperidin (0.162). Therefore, the estimated contribution of each antioxidant to the total antioxidant capacity of 100 g of fresh yuzus is as follows (in mg of VCEAC): vitamin C (90.36 mg) > naringin (12.44 mg) > hesperidin (10.64 mg). Our results indicate that mature yuzu contains higher amounts of vitamin C and phenolics than other citrus fruits and could therefore be

  6. Anticancer and Antioxidant Activity of Bread Enriched with Broccoli Sprouts

    Science.gov (United States)

    Gawlik-Dziki, Urszula; Świeca, Michał; Dziki, Dariusz; Sęczyk, Łukasz; Złotek, Urszula; Różyło, Renata; Kaszuba, Kinga; Ryszawy, Damian; Czyż, Jarosław

    2014-01-01

    This study is focused on antioxidant and anticancer capacity of bread enriched with broccoli sprouts (BS) in the light of their potential bioaccessibility and bioavailability. Generally, bread supplementation elevated antioxidant potential of product (both nonenzymatic and enzymatic antioxidant capacities); however, the increase was not correlated with the percent of BS. A replacement up to 2% of BS gives satisfactory overall consumers acceptability and desirable elevation of antioxidant potential. High activity was especially found for extracts obtained after simulated digestion, which allows assuming their protective effect for upper gastrointestinal tract; thus, the anticancer activity against human stomach cancer cells (AGS) was evaluated. A prominent cytostatic response paralleled by the inhibition of AGS motility in the presence of potentially mastication-extractable phytochemicals indicates that phenolic compounds of BS retain their biological activity in bread. Importantly, the efficient phenolics concentration was about 12 μM for buffer extract, 13 μM for extracts after digestion in vitro, and 7 μM for extract after absorption in vitro. Our data confirm chemopreventive potential of bread enriched with BS and indicate that BS comprise valuable food supplement for stomach cancer chemoprevention. PMID:25050366

  7. Lipophilization of Resveratrol and Effects on Antioxidant Activities.

    Science.gov (United States)

    Oh, Won Young; Shahidi, Fereidoon

    2017-10-04

    Resveratrol (R), a polyphenol, was structurally modified via esterification with selected fatty acids to expand its potential application in lipophilic foods, drugs, and cosmetics. The esterification was carried out using 12 different fatty acids with varying chain lengths and degrees of unsaturation (C3:0-C22:6). Two monoesters, two diesters, and one triester were identified by high-performance liquid chromatography-mass spectrometry, and the monoesters (R-3-O-monodocosahexaenoate and R-4'-O-monodocosahexaenoate) were structurally confirmed by nuclear magnetic resonance. The lipophilicity of resveratrol and its alkyl esters was calculated using ALOGPS 2.1. Resveratrol exhibited greater antioxidant activity in both 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging assays. Resveratrol esters with long-chain fatty acids (C18:0 and C18:1) showed higher antioxidant activity in the DPPH radical scavenging assay, whereas short-chain fatty acid (C3:0, C4:0, and C6:0) showed higher antioxidant activity in the ABTS radical cation scavenging assay. The results may imply that resveratrol derivatives could be used in lipophilic media as health beneficial antioxidants.

  8. Antioxidant activity of ethanolic extracts from several asparagus cultivars.

    Science.gov (United States)

    Rodríguez, Rocío; Jaramillo, Sara; Rodríguez, Guillermo; Espejo, Juan Antonio; Guillén, Rafael; Fernández-Bolaños, Juan; Heredia, Antonia; Jiménez, Ana

    2005-06-29

    Three different methods (antiradical activity, inhibition of primary oxidation, and ferric reducing power) have been used to evaluate the antioxidant activity of eight different asparagus cultivars and byproducts: white and green asparagus from Alcalá del Río (Guadalquivir Valley, Seville) and American hybrids, native spears, and their byproducts from Huétor-Tájar (Vega de Granada). The correlation between antioxidant activity and total phenol content was studied. Six standards were also tested to validate the modified methods for antioxidant activity determination. Results obtained for antiradical capacity and reducing power were very similar, and a high correlation with phenols was found (R > or = 0.9 for both tests). Sample origin was an important factor, spears from Huétor-Tájar having higher values (ARC between 7 and 10 and P(R) of 0.25-0.33) than those from Alcalá del Río (ARC 0.6-2 and P(R) of 0.05-0.07). Significant differences were found between spears with the same origin, suggesting that genetics are another factor to take into account. Asparagus inhibits lipid primary oxidation, but no correlation between the inhibition percentage and phenols was observed. Asparagus origin was the only factor that led to significant differences: samples from Huétor-Tájar had higher values (POIC between 18 and 32) than those from Alcalá del Río (POIC of 5-9). Byproducts from the canning industry at Huétor-Tájar were also assayed for antioxidant activity; the results obtained suggested that byproducts could be considered as an excellent source of natural antioxidants.

  9. Antioxidant activity and high-performance liquid chromatographic analysis of phenolic compounds during in vitro callus culture of Plantago ovata Forsk. and effect of exogenous additives on accumulation of phenolic compounds.

    Science.gov (United States)

    Talukder, Pratik; Talapatra, Shonima; Ghoshal, Nirmalya; Sen Raychaudhuri, Sarmistha

    2016-01-15

    Plantago ovata, commonly called psyllium, is known to be a rich source of polyphenolic compounds. The present study was aimed at determining polyphenol content and studying their antioxidant activities in P. ovata during in vitro callus culture. An attempt was also made to enhance polyphenol content using external additives. The role of PAL gene in polyphenol accumulation was also studied. The study indicated the presence of significant amounts of polyphenols, including flavonoids, in P. ovata callus. A gradual increase in polyphenol and flavonoid content was observed up to the third passage (63 days) of callus culture, which declined at the next passage. The third-passage callus showed highest antioxidant activity. High-performance liquid chromatographic results indicated the presence of high amounts of gallic acid and rutin in P. ovata calli; however, other polyphenols were also present but to a lesser extent. Additive supplementation was effective in enhancing polyphenol production and in increasing antioxidant activity in P. ovata callus. The present research reported accumulation of polyphenols in callus culture of P. ovata, which could be applied to isolation of polyphenols for various beneficial purposes. It also indicated enhancement in the production of several polyphenols and also an increase in antioxidant activity in the additive-treated callus. © 2015 Society of Chemical Industry.

  10. Antioxidant Activities of Various Extracts from Artemisisa selengensis Turcz (LuHao

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    Yan Chen

    2010-07-01

    Full Text Available The antioxidant activities of the various extracts and fractions from the herbs of Artemisia selegensis Turcz (AST were investigated by in vitro and in vivo assays. FRAP, DPPH and ABTS assays were used to evaluate the antioxidant activities of the extracts/fractions in vitro. The effect of water extract (WE in reducing oxidative stress in male mice was evaluated. Phenolic acid compounds contribute significantly to the antioxidant activity. From the results of three in vitro antioxidant assays, WE was found to have the highest antioxidant activity, and among the WE subfractions, the water soluble fraction has a significant antioxidant activity. The in vivo antioxidant assay results showed that high doses of WE significantly decrease the MDA level compared to normal diet and D-(+ galactose group (p < 0.05, and the SOD activity of mice given a high dose of WE was the highest. These in vitro and in vivo studies demonstrated that the extracts, especially the WE from AST, have significant antioxidant and free radical scavenging activities. In summary, we propose that AST could be potentially used as a rich source of natural antioxidants.

  11. Major factors influencing antioxidant contents and antioxidant activity in grapes and wines

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    Jaromír Lachman

    2009-03-01

    Full Text Available Jaromír Lachman, Miloslav Šulc, Katerina Faitová, Vladimír PivecDepartment of Chemistry, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech RepublicAbstract: Phenolic compounds in wines, especially in red wines, possess strong antioxidant activity, have the largest effect in decreasing atherosclerosis by both hypolipemic and antioxidant mechanisms. The long-term uptake of red wine has a positive impact on antioxidant activity (AA of blood plasma in rats in vivo and increases AA by 15%–20% compared to a control group. In the article the effect of total phenolics (TP, total anthocyanins (TA, individual anthocyanins, procyanidins and phenolics contained in red grapes, musts, grape seeds and skins and wines on the AA is discussed. Significant impact of varieties, viticultural regions and locations, climate conditions and vintage has been shown. Likewise, the ways and individual stages of the vinification technology process, and storage conditions affect color, TP, TA, and AA and health aspects of produced wines. Resveratrol, another free radical scavenger mainly contained in the skins of grapes, inhibits the risk of cardiovascular diseases. Higher amounts of trans-resveratrol (RES have been found in wines from cool and wet climate regions and lesser amounts are typical for warm and dry regions. Changes in the TP content and AA affected by grape variety, vineyard location and winemaking process in white and blue varieties from different vineyards of the Czech Republic were studied. Significant differences in TP among varieties were found. Analysis of variance showed statistically high differences among red and white wines and growing locations. Wines differed significantly in TP content and AA increased significantly during the winemaking process. Statistically significant differences in AA values were found among growing areas, wines and varieties. Significant positive correlations between TP

  12. Evaluation of extracts of leaf of three Ficus deltoidea varieties for antioxidant activities and secondary metabolites

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    Zunoliza Abdullah

    2009-01-01

    Full Text Available Present study aimed to investigate alcoholic and aqueous extracts of three varieties of Ficus deltoidea for antioxidant activity using in vitro models such as free radical scavenging activity, reduction power of iron (III, superoxide anion (O2 scavenging, xanthine oxidase (XOD, nitric oxide (NO· and lipid peroxidation. The total polyphenols, flavonoids and tannins were estimated using colorimetry in order to investigate their correlation with antioxidant activities. Alcoholic and aqueous extracts of different varieties of the plant exhibited different radical scavenging activities in different models (P< 0.05. Both the types of extracts displayed high antioxidant activity in DPPH and superoxide anion scavenging models, and the activity was comparable to quercetin, rutin, butylated hydroxyanisole (BHA, ascorbic acid and allopurinol. A correlation was observed between antioxidant activity and content of total polyphenols, flavonoids and tannins. The results of this study indicate that the extracts of Ficus deltoidea possess promising antioxidant activity.

  13. Antioxidant activity of the medicinal plant Enicostemma littorale Blume

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    P Abirami

    2011-01-01

    Full Text Available Medicinal plants are the source for wide variety of natural antioxidants. In the study reported here, we have conducted a comparative study between the different parts of the plant Enicostemma littorale. The amount of total phenols and antioxidant enzymes Glutathione-S-Transferase, Superoxide Dismutase, Catalase and Peroxidase activities were evaluated and also the non-enzymatic antioxidants ascorbic acid, α- tocopherol and Glutathione activities were evaluated. The results showed that the antioxidant activities varied greatly among the different plant parts used in this study and some parts are rich in natural antioxidants especially the flowers of E. littorale. These results suggest that Enicostemma littorale have strong antioxidant potential. Further study is necessary for isolation and characterization of antioxidant agents, which can be used to treat various oxidative stress-related diseases.

  14. Antioxidant activity and analysis of proanthocyanidins from pine (Pinus densiflora) needles.

    Science.gov (United States)

    Park, Yong Soo; Jeon, Min Hee; Hwang, Hyun Jung; Park, Mi Ra; Lee, Sang-Hyeon; Kim, Sung Gu; Kim, Mihyang

    2011-08-01

    In this study, we evaluated the antioxidant activity of pine needle extracts prepared with hot water, ethanol, hexane, hot water-hexane (HWH), and hot water-ethanol (HWE), using the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical method. The hot water extract possessed superior antioxidant activity than the other extracts. We also compared the antioxidant activity of pine needle extracts through ROS inhibition activity in a cellular system using MC3T3 E-1 cells. The hot water extract exhibited the lowest ROS production. The pattern of HPLC analysis of each extract indicated that the hot water extract contained the highest proanthocyanidin level. The pine needle hot-water extract was then isolated and fractionated with Sephadex LH-20 column chromatography to determine the major contributor to its antioxidant activity. The No.7 and 12 fractions had high antioxidant activities, that is, the highest contents of proanthocyanidins and catechins, respectively. These results indicate that the antioxidant activity of procyanidins from the hot water extract of pine needles is positively related to not only polymeric proanthocyanidins but also to monomeric catechins. Moreover, the antioxidant activity of the pine needle hot water extract was similar to well-known antioxidants, such as vitamin C. This suggests that pine needle proanthocyanidins and catechins might be of interest for use as alternative antioxidants.

  15. In vivo photoprotective and anti-inflammatory effect of hyperforin is associated with high antioxidant activity in vitro and ex vivo.

    Science.gov (United States)

    Meinke, Martina C; Schanzer, Sabine; Haag, Stefan F; Casetti, Federica; Müller, Marcel L; Wölfle, Ute; Kleemann, Anke; Lademann, Juergen; Schempp, Christoph M

    2012-06-01

    Hyperforin, a major constituent of St. John's Wort (Hypericum perforatum, HP), provides anti-inflammatory, anti-tumor, and anti-bacterial properties. Previous studies have shown anti-oxidative properties of St. John's Wort extracts; however, its free radical scavenging activity in skin cells or skin has not been assessed in detail so far. Therefore, the free radical scavenging activity of hyperforin was tested in the H(2)DCFDA-assay in vitro in HaCaT keratinocytes irradiated with solar simulated radiation. Hyperforin (EC(50) 0.7 μM corresponding to 0.42 μg/ml) was much more effective compared to Trolox (EC(50) 12 μg/ml) and N-acetylcysteine (EC(50) 847 μg/ml) without showing phototoxicity. The radical protection factor of a cream containing 1.5%w/w of a hyperforin-rich HP extract was determined to be 200 × 10(14) radicals/mg, indicating a high radical scavenging activity. The cream was further applied ex vivo on porcine ear skin and significantly reduced radical formation after infrared irradiation. Finally, the UV-protective effect of the HP cream was tested on 20 volunteers in a randomized, double-blind, vehicle-controlled study. HP cream significantly reduced UVB-induced erythema as opposed to the vehicle. Occlusive application of HP cream on non-irradiated test sites did not cause any skin irritation. Taken together, these results demonstrate that hyperforin is a powerful free radical scavenger. Copyright © 2012 Elsevier B.V. All rights reserved.

  16. Antioxidant activity of the probiotic consortium in vitro

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    Saule Saduakhasova

    2014-01-01

    Full Text Available Introduction: Available evidence suggests that probiotics have different biological functions that depend on several mechanisms, such as antioxidant and DNA-protective activities. The probiotic consortium includes bacterial cultures such as Streptococcus thermophilus, Lactococcus lactis, Lactobacillus plantarum, and other bacterial cultures isolated from traditional Kazakh dairy products (ayran, kumys, shubat, and healthy clinical material. The aim of this study was to investigate the total antioxidant activity of the consortium of probiotic bacteria and to determine the activity of superoxide dismutase, glutathione reductase, and DNA-protective action. Material and methods: In vitro comet assay was used to determine the antigenotoxicity of the probiotic consortium. Total antioxidant activity was determined using a method of analysis with Trolox as the equivalent. The analysis method of superoxide dismutase activity assesses the inhibition rate of the nitroblue tetrazolium reduction to formazan by superoxide dismutase. Determination of glutathione reductase activity is based on the measurement of the NADPH oxidation speed. Results: A significantly high level of the total antioxidant activity of the probiotic consortium intact cells (15.3 mM/ml was observed whereas the activity index of  lysate  was 11.1 mM/ml. The superoxide dismutase activity of probiotic consortium lysate was evaluated, with values that peaked at 0.24 U/mg protein. The superoxide dismutase activity of the consortium was lower in comparison to L.fernentum E-3 and L.fernentum E-18 cultures with values of 0.85 U/mg and 0.76 U/mg protein, respectively. SOD activity of probiotic consortium whole cells was not observed, which is typical for lactic acid bacteria. Glutathione reductase plays an important role in the optimal protection from oxidative stress. Glutathione reductase activity of the studied probiotic consortium was low; moreover, the activity of the lysate was two times

  17. [Effects of nitrogen application period on the nitrogen metabolism key enzymes activities and antioxidant characteristics of high-yielding summer maize].

    Science.gov (United States)

    Lü, Peng; Zhang, Ji-Wang; Liu, Wei; Yang, Jin-Sheng; Dong, Shu-Ting; Liu, Peng; Li, Deng-Hai

    2012-06-01

    Taking the high-yielding summer maize cultivars Denghai 661 (DH661) and Zhengdan 958 (ZD958) as test materials, a field experiment was conducted to study their grain yield, nitrogen use efficiency, key enzymes activities of nitrogen metabolism, and antioxidant enzymes activities under effects of different nitrogen application periods. One-dose nitrogen application at jointing stage was not beneficial to the increase of grain yield and the nitrogen accumulation in plant and grain, while split application in combination with application after anthesis increased the nitrogen accumulation in plant and grain significantly and increased the grain yield. When the nitrogen was applied at a ratio of 2:4:4 at jointing stage, 10-leaf stage, and 10 days after anthesis, the grain yield of DH661 was up to 14123.0 kg x hm(-2); when the nitrogen was applied at a ratio of 1:2:5:2 as the basal and at jointing stage, 10-leaf stage, and 10 days after anthesis, the grain yield of ZD958 was up to 14517.1 kg x hm(-2). These two nitrogen application modes increased the grain yield of DH661 and ZD958 by 14.5% and 17.5%, respectively, as compared with one-dose nitrogen application at jointing stage. Split nitrogen application before anthesis increased plant nitrate reductase activity significantly. In the 0-42 days after anthesis under split nitrogen application, the glutamine synthetase, glutamate synthase, and glutamate dehydrogenase activities of DH661 and ZD958 were averagely increased by 32.6%, 47.1% and 50.4%, and 14.5%, 61.8% and 25.6%, and the superoxide dismutase and catalase activities were increased by 22. 0% and 36.6%, and 13.4% and 62.0%, respectively, and the malondialdehyde content was decreased significantly, as compared with one-off nitrogen application. It was suggested that for the high-yielding of summer maize, split application of nitrogen and appropriately increasing the nitrogen application ratio after anthesis could enhance the plant key nitrogen metabolism enzymes

  18. Phytochemical profile, toxicity and antioxidant activity of Aloysia gratissima (Verbenaceae)

    OpenAIRE

    Zeni,Ana Lúcia B.; Albuquerque,Cláudia Almeida C. de; Filipe Gonçalves; Alexandra Latini; Tasca, Carla I.; Rossana Podestá; Pagliosa,Cristiane M.; DUARTE,FILIPE S.; Lima,Thereza C. M.; Marcelo Maraschin

    2013-01-01

    Aloysia gratissima (Gill. et Hook) Tronc. (Verbenaceae) is native to South America with folk therapeutic applications for a wide range of diseases. The polyphenolic and carotenoid profile, toxicity, and antioxidant activity of aqueous extract of Aloysia gratissima were investigated. HPLC analyses showed high amounts of ferulic acid, trans-cinnamic acid and p-coumaric acid, and also trans-β- carotene and lutein which fluctuated throughout the seasons. Furthermore, the extract investigated...

  19. Antioxidant and antimicrobial activities of Shorea kunstleri

    Institute of Scientific and Technical Information of China (English)

    Siti Suria Daud; Muhammad Taher; Deny Susanti

    2014-01-01

    Objective:To evaluate antioxidant and antimicrobial activities of stembark of Shorea kunstleri (S. kunstleri) together with analysis of phytochemical and total phenolic contents. Methods:Extraction was conducted with different solvent polarity of n-hexane, dichloromethane (DCM) and methanol by using Soxhlet extraction. Total phenolic content was determined using Folin-Ciocalteu method. Free radical scavenging activity and inhibition of lipid peroxidation were evaluated with DPPH radical scavenging and ferric thiocyanate assays, respectively. Antimicrobial activities were performed using disc diffusion method, minimum inhibition concentration (MIC), minimum bactericidal concentration (MBC), and minimum fungicidal concentration. Results:S. kunstleri stembark extracts revealed presence of steroids, terpenoids, saponins, flavonoids, and phenolic compounds. Methanol extract exhibited the highest total phenolic content and free radical scavenging activity resulting in phenolic content of (8.340±0.003) g GAE/100 g of extract and (95.90±1.07)% DPPH inhibition (IC50 value of 18.6 µg/mL), respectively. Ferric thiocyanate assay of n-hexane, DCM, and methanol extracts indicated lipid peroxidation inhibitory activity of (74.20±0.35)%, (74.00±0.10)%, and (72.80±0.27)%, respectively. In antimicrobial and antifungal tests, methanol extract showed inhibition against Staphylococcus aureus (S. aureus), Candida albicans, and Candida tropicalis with inhibition zones of 10-12, 18-22, and 18-19 mm, respectively. The MIC test of methanol extract showed highest inhibition against Candida albicans and S. aureus (0.04 and 0.08 mg/mL, respectively) while DCM extract exhibited the highest activity towards Candida tropicalis (MIC value of 0.63 mg/mL). Taken together, MBC test of methanol extract strongly demonstrated bactericidal effect against S. aureus with MBC value of 0.08 mg/mL. Conclusions:The study demonstrated that stembark extracts of S. kunstleri possessed antioxidant and

  20. Antioxidant and antimicrobial activities of Shorea kunstleri

    Directory of Open Access Journals (Sweden)

    Siti Suria Daud

    2014-08-01

    Full Text Available Objective: To evaluate antioxidant and antimicrobial activities of stembark of Shorea kunstleri (S. kunstleri together with analysis of phytochemical and total phenolic contents. Methods: Extraction was conducted with different solvent polarity of n-hexane, dichloromethane (DCM and methanol by using Soxhlet extraction. Total phenolic content was determined using Folin-Ciocalteu method. Free radical scavenging activity and inhibition of lipid peroxidation were evaluated with DPPH radical scavenging and ferric thiocyanate assays, respectively. Antimicrobial activities were performed using disc diffusion method, minimum inhibition concentration (MIC, minimum bactericidal concentration (MBC, and minimum fungicidal concentration. Results: S. kunstleri stembark extracts revealed presence of steroids, terpenoids, saponins, flavonoids, and phenolic compounds. Methanol extract exhibited the highest total phenolic content and free radical scavenging activity resulting in phenolic content of (8.340±0.003 g GAE/100 g of extract and (95.90±1.07% DPPH inhibition (IC50 value of 18.6 µg/mL, respectively. Ferric thiocyanate assay of n-hexane, DCM, and methanol extracts indicated lipid peroxidation inhibitory activity of (74.20±0.35%, (74.00±0.10%, and (72.80±0.27%, respectively. In antimicrobial and antifungal tests, methanol extract showed inhibition against Staphylococcus aureus (S. aureus, Candida albicans, and Candida tropicalis with inhibition zones of 10-12, 18-22, and 18-19 mm, respectively. The MIC test of methanol extract showed highest inhibition against Candida albicans and S. aureus (0.04 and 0.08 mg/mL, respectively while DCM extract exhibited the highest activity towards Candida tropicalis (MIC value of 0.63 mg/mL. Taken together, MBC test of methanol extract strongly demonstrated bactericidal effect against S. aureus with MBC value of 0.08 mg/mL. Conclusions: The study demonstrated that stembark extracts of S. kunstleri possessed antioxidant

  1. ANTIOXIDANT AND ANTIDIABETIC ACTIVITY OF CURCUMA AROMATICA

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    Ammayappan Rajam Srividya

    2012-06-01

    Full Text Available The objective of this paper is to find out the antidiabetic activity of Curcuma aromatica. In this research paper we dealt with antioxidant activities by DPPH method, ABTS method, Lipid peroxidation assay and scavenging ability of the extract for the hydrogen peroxide radical, Glucose uptake by rat hemi diaphragm method. Antidiabetic activity using healthy adult Wister rats were also carried out. Toluene extract of Curcuma aromatica showed the potent scavenging activity by DPPH method with the IC 50 value of 50.62±0.998 µg/ml, by lipid per oxidation method with the IC 50 value of 75±0.87 µg/ml, hydrogen peroxide radical scavenging activity with the IC 50 value 43.75±1.24 µg/ml, and ABTS radical scavenging method with the IC 50 value 0.038±1.54 µg/ml. After the treatment with the toluene extract of Curcuma aromatica, serum glucose level was found to be decreased from 278.53 to 116.5 mg/dl, total protein level increased from 3.09 to 5.78 mg/dl. There was a decrease in total cholesterol level from 292.33 to 134.50 mg/dl, decrease in serum triglyceride level from 85.66 to 64.16mg/dl when compared to diabetic control group. Toluene extract of Curcuma aromatica exhibited significant antioxidant and antidiabetic activities in both in vitro and in vivo models. So, it can be used as alternative herbal medicine in the treatment of diabetes and diabetic induced complication.

  2. Structural elucidation and cellular antioxidant activity evaluation of major antioxidant phenolics in lychee pulp.

    Science.gov (United States)

    Su, Dongxiao; Ti, Huihui; Zhang, Ruifen; Zhang, Mingwei; Wei, Zhengchen; Deng, Yuanyuan; Guo, Jinxin

    2014-09-01

    Lychee pulp contains phenolic compounds that are strong antioxidants, but the identities of the major antioxidants present are unknown. In the present study, the major contributors to the antioxidant activity of fresh lychee pulp were identified and their cellular antioxidant activities were investigated. Aqueous acetone extracts of lychee pulp were fractionated on polyamide resin, and those fractions with the largest antioxidant and radical scavenging activities were selected using cellular antioxidant activity and oxygen radical absorbance capacity assays. Three compounds that were major contributors to the antioxidant activity in these fractions were obtained by reverse-phase preparative HPLC and identified as quercetin 3-O-rutinoside-7-O-α-L-rhamnosidase (quercetin 3-rut-7-rha), quercetin 3-O-rutinoside (rutin) and (-)-epicatechin using NMR spectroscopy, HMBC, and ESI-MS spectrometry. The concentration of quercetin 3-rut-7-rha was 17.25mg per 100g of lychee pulp fresh weight. This is the first report of the identification and cellular antioxidant activity of quercetin 3-rut-7-rha from lychee pulp.

  3. Studies on the antioxidant activities of some new chromone compounds.

    Science.gov (United States)

    Kładna, Aleksandra; Berczyński, Paweł; Piechowska, Teresa; Kruk, Irena; Aboul-Enein, Hassan Y; Ceylan-Unlusoy, Meltem; Verspohl, Eugen J; Ertan, Rahmiye

    2014-11-01

    Recent reviews evidence that the naturally occurring compounds containing the chromone skeleton exhibit antiradical activities, providing protection against oxidative stress. The antioxidant activities of 13 new synthesized chromonyl-2,4-thiazolidinediones, chromonyl-2,4-imidazolidinediones and chromonyl-2-thioxoimidzolidine-4-ones were evaluated using in vitro antioxidant assays, including superoxide anion radical (O2(-•)), hydroxyl radical (HO(•)), 2,2-diphenyl-1-picryl-hydrazyl free radical (DPPH(•)) scavenging capacity and total antioxidant capacity ferric ion reducing activity. Superoxide anion radical was produced using potassium superoxide/18-crown-6-ether dissolved in dimethylsulfoxide, and the Fenton-like reaction (Fe(II) + H2O2) was a generator of hydroxyl radicals. Chemiluminescence, spectrophotometry, electron paramagnetic resonance (EPR) and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as the spin trap were the measurement techniques. The results showed that the majority of the chromone derivatives tested showed a strong scavenging effect towards free radicals, similar to the chemiluminescence reaction with superoxide anion radical with a high activity, inhibition of the DMPO-OOH radical EPR signal (24-58%), the DMPO-OH radical EPR signal (4-75%) and DPPH radical EPR signal (6-100%) at 1 mmol/L. Several of the examined compounds exhibited the high reduction potentials. The results obtained show that the new synthesized chromone derivatives may directly scavenger reactive oxygen species and thus may play a protective role against oxidative damage. Copyright © 2014 John Wiley & Sons, Ltd.

  4. Edible flowers — antioxidant activity and impact on cell viability

    National Research Council Canada - National Science Library

    Kucekova, Zdenka; Mlcek, Jiri; Humpolicek, Petr; Rop, Otakar

    2013-01-01

    The phenolic compound composition, antioxidant activity and impact on cell viability of edible flower extracts of Allium schoenoprasum; Bellis perennis; Cichorium intybus; Rumex acetosa; Salvia pratensis; Sambucus nigra...

  5. Antioxident activity of the mangrove endophytic fungus (Trichoderma sp.)

    Institute of Scientific and Technical Information of China (English)

    Saravanakumar Kandasamy; Kathiresan Kandasamy

    2014-01-01

    Objective: To test antioxidant property of the endophytic Trichoderma species isolated from the leaves of 12 mangroves of Andaman Nicobar Islands. Methods: Eight strains of Trichoderma species were found predominant and their crude extracts were assessed for antioxidant activity by using seven assays.Results:EMFCAS8 and other strains also showed considerable activity. Total antioxidant activity varied with the strains and it was maximum in Trichoderma Conclusions: This work concluded that mangroves are rich in endophytic Trichoderma species with potential for antioxidant activity.

  6. Antioxidant activity and phenolic content of leaf infusions of Myrtaceae species from Cerrado (Brazilian Savanna).

    Science.gov (United States)

    Takao, L K; Imatomi, M; Gualtieri, S C J

    2015-11-01

    There is considerable interest in identifying new antioxidants from plant materials. Several studies have emphasized the antioxidant activity of species belonging to the Myrtaceae family. However, there are few reports on these species from the Cerrado (Brazilian savanna). In this study, the antioxidant activity and phenolic content of 12 native Myrtaceae species from the Cerrado were evaluated (Blepharocalyx salicifolius, Eugenia bimarginata, Eugenia dysenterica, Eugenia klotzschiana, Hexachlamys edulis, Myrcia bella, Myrcia lingua, Myrcia splendens, Myrcia tomentosa, Psidium australe, Psidium cinereum, and Psidium laruotteanum). Antioxidant potential was assessed using the antioxidant activity index (AAI) by the DPPH method and total phenolic content (TPC) by the Folin-Ciocalteu assay. There was a high correlation between TPC and AAI values. Psidium laruotteanum showed the highest TPC (576.56 mg GAE/g extract) and was the most potent antioxidant (AAI = 7.97, IC50 = 3.86 µg·mL-1), with activity close to that of pure quercetin (IC50 = 2.99 µg·mL-1). The extracts of nine species showed IC50 of 6.24-8.75 µg·mL-1. Most species showed TPC and AAI values similar to or higher than those for Camellia sinensis, a commonly consumed tea with strong antioxidant properties. The results reveal that the analyzed Myrtaceae species from the Cerrado possess high phenolic contents and antioxidant activities. Thus, they are a potential source of new natural antioxidants.

  7. Antioxidant activity and phenolic content of leaf infusions of Myrtaceae species from Cerrado (Brazilian Savanna

    Directory of Open Access Journals (Sweden)

    L. K. Takao

    Full Text Available Abstract There is considerable interest in identifying new antioxidants from plant materials. Several studies have emphasized the antioxidant activity of species belonging to the Myrtaceae family. However, there are few reports on these species from the Cerrado (Brazilian savanna. In this study, the antioxidant activity and phenolic content of 12 native Myrtaceae species from the Cerrado were evaluated (Blepharocalyx salicifolius, Eugenia bimarginata, Eugenia dysenterica, Eugenia klotzschiana, Hexachlamys edulis, Myrcia bella, Myrcia lingua, Myrcia splendens, Myrcia tomentosa, Psidium australe, Psidium cinereum, and Psidium laruotteanum. Antioxidant potential was assessed using the antioxidant activity index (AAI by the DPPH method and total phenolic content (TPC by the Folin–Ciocalteu assay. There was a high correlation between TPC and AAI values. Psidium laruotteanum showed the highest TPC (576.56 mg GAE/g extract and was the most potent antioxidant (AAI = 7.97, IC50 = 3.86 µg·mL−1, with activity close to that of pure quercetin (IC50 = 2.99 µg·mL−1. The extracts of nine species showed IC50 of 6.24–8.75 µg·mL−1. Most species showed TPC and AAI values similar to or higher than those for Camellia sinensis, a commonly consumed tea with strong antioxidant properties. The results reveal that the analyzed Myrtaceae species from the Cerrado possess high phenolic contents and antioxidant activities. Thus, they are a potential source of new natural antioxidants.

  8. Cloud-point extraction and reversed-phase high performance liquid chromatography for analysis of phenolic compounds and their antioxidant activity in Thai local wines.

    Science.gov (United States)

    Vichapong, Jitlada; Santaladchaiyakit, Yanawath; Burakham, Rodjana; Srijaranai, Supalax

    2014-04-01

    A cloud-point extraction (CPE) was developed for the determination of 12 phenolic compounds (i.e. gallic acid, procatechuic acid, p-hydroxybenzoic acid, vanillic acid, caffeic acid, syringic acid, p-coumaric acid, ferulic acid, guaiacol, p-cresol, o-cresol and 3,5-xylenol) using reversed-phase high performance liquid chromatography (RP-HPLC) with photodiode array (PDA) detection. The optimum CPE conditions were 2.0% (w/v) Triton X-114, 3.0% (w/v) Na2SO4 and 20-min equilibrated at 45 °C. The surfactant-rich phase was then analyzed by HPLC using a Symmetry C18 column, gradient mobile phase of acetonitrile and 1% (v/v) acetic acid, and PDA detection at 280 nm. Under the optimum condition, the target phenolic compounds were separated within 25 min. CPE gave higher enrichment factor up to 15-fold compared to that of direct analysis. The proposed method showed good analytical performances with limits of detection in the range 0.01-0.1 mg L(-1) and precisions with relative standard deviation (RSD) lower than 5% for retention time and 10% for peak area. The method was successfully applied to the analysis of phenolic compounds in Thai local wine samples. Gallic acid, procatechuic acid, and vanillic acid were the highest phenolics found in the studied wines with the contents up to 172.4, 99.1, and 26.6 mg L(-1), respectively. The recovery of the spiked wine samples (0.5, 1.0, and 2.0 mg L(-1)) were obtained in the range of 90.4-110%. High total phenolic content, total flavonoids, and antioxidant activity (DPPH method) in the studied wines were also observed.

  9. Synthesis and antioxidant activity of star-shape phenolic antioxidants catalyzed by acidic nanocatalyst based on reduced graphene oxide.

    Science.gov (United States)

    Golestanzadeh, Mohsen; Naeimi, Hossein; Zahraie, Zohreh

    2017-02-01

    Phenolic antioxidants play important role in prevention of oxidation in different industrials. The research objective in the current study was synthesis and evaluate of antioxidant activity of star-shape phenolic antioxidants. The synthetic compounds were prepared in the presence of sulfonated reduced graphene oxide. The antioxidant activity of synthesized compounds was investigated by spectrophotometrically method according to the DPPH assay. Overall, these compounds are potentially important antioxidant and also to limit activity of reactive oxygen species.

  10. Antioxidant Activity of Methanolic Extracts from Peanut Skin

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    V. Nepote

    2000-03-01

    Full Text Available Antioxidant activity of skin from runner peanut was performed on sunflower refined oil. The skin was obtained from industrial blanching process. The oil was oxidized at 60ºC. The methanolic extracts show antioxidant activity in relation to the oil (without additives. However these extracts do not reach the activity level from BHT.

  11. Novel macromolecules derived from coumarin: synthesis and antioxidant activity

    Science.gov (United States)

    Al-Amiery, Ahmed A.; Al-Majedy, Yasameen K.; Kadhum, Abdul Amir H.; Mohamad, Abu Bakar

    2015-07-01

    The rational design of 4-hydroxycoumarins with tailor-made antioxidant activities is required nowadays due to the wide variety of pharmacologically significant, structurally interesting of coumarins and researcher orientation toward green chemistry and natural products. A simple and unique coumarins have been achieved by reaction of 4-hydroxycoumarin with aromatic aldehyde accompanied with the creation of a macromolecules have 2-aminothiazolidin-4-one. The molecular structures of the compounds were characterized by the Fourier transformation infrared and Nuclear magnetic resonance spectroscopies, in addition to CHN analysis. The scavenging abilities of new compounds against stable DPPH radical (DPPH•) and hydrogen peroxide were done and the results show that the compounds exhibited high antioxidant activates.

  12. The Antioxidant Activity of New Coumarin Derivatives

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    Abu Bakar Mohamad

    2011-09-01

    Full Text Available The antioxidant activity of two synthesized coumarins namely, N-(4,7-dioxo-2-phenyl-1,3-oxazepin-3(2H,4H,7H-yl-2-(2-oxo-2H-chromen-4-yloxyacetamide 5 and N-(4-oxo-2-phenylthiazolidin-3-yl-2-(2-oxo-2H-chromen-4-yloxyacetamide 6 were studied with the DPPH, hydrogen peroxide and nitric oxide radical methods and compared with the known antioxidant ascorbic acid. Compounds 5 and 6 were synthesized in a good yield from the addition reaction of maleic anhydride or mercaptoacetic acid to compound 4, namely N'-benzylidene-2-(2-oxo-2H-chromen-4-yloxyacetohydrazide. Compound 4 was synthesized by the condensation of compound 3, namely 2-(2-oxo-2H-chromen-4-yloxy acetohydrazide, with benzaldehyde. Compound 3, however, was synthesized from the addition of hydrazine to compound 2, namely ethyl 2-(2-oxo-2H-chromen-4-yloxyacetate, which was synthesized from the reaction of ethyl bromoacetate with 4-hydroxycoumarin 1. Structures for the synthesized coumarins 2–6 are proposed on the basis of spectroscopic evidence.

  13. Standardization and in vitro antioxidant activity of jatamansi rhizome

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    Mhaveer Singh

    2015-01-01

    Full Text Available Background: Nardostachys jatamansi Linn. commonly known as jatamansi is a well notorious drug in Indian systems of medicines having various health-related benefits and employed in various herbal formulations due to the presence of high levels of valuable phenolic constituents. The present study was aimed to quality assessment of Jatamansi rhizome by studying macro- and micro-scopic characters along with physicochemical tests, chemo-profiling using thin layer chromatography (TLC, and gas chromatography–mass spectrometry (GC-MS, in vitro antioxidant activity. Materials and Methods: Standardization was carried out as per the pharmacopeial guidelines and contaminant estimation was carried out by analyzing the samples for the determination of heavy metals, pesticides, and aflatoxins. Chemo-profiling was done with TLC by optimizing the mobile phase for different extracts. The GC-MS chemo-profiling was also carried out by using hexane soluble fraction of the hydroalcoholic extract. The drug is well known for a protective role in the human body as an antioxidant, so total phenolic contents and in vitro antioxidant efficacy was also determined by using established methods. Results:The results of quality control and anatomical studies were very much useful for its identification, whereas significant antioxidant efficacy was also observed. The drug was found free of contaminants when analyzed for pesticides and aflatoxins, whereas heavy metals were found under the pharmacopeial limit. Conclusion: The findings of the present research can be utilized for the identification and quality control of the jatamansi rhizome.

  14. Mineral Components and Anti-oxidant Activities of Tropical Seaweeds

    Institute of Scientific and Technical Information of China (English)

    Suzuki Takeshi; Yoshie-Stark Yumiko; Santoso Joko

    2005-01-01

    Seaweeds are known to hold substances of high nutritional value; they are the richest resources of minerals important to the biochemical reactions in the human body. Seaweeds also hold non-nutrient compounds like dietary fiber and polyphenols. However, there is not enough information on the mineral compounds of tropical seaweeds. Also we are interested in the antioxidant activities of seaweeds, especially those in the tropical area. In this study, Indonesian green, brown and red algae were used as experimental materials with their mineral components analyzed by using an atomic absorption spectrophotometer. The catechins and flavonoids of these seaweeds were extracted with methanol and analyzed by high performance liquid chromatography (HPLC); the anti-oxidant activities of these seaweeds were evaluated in a fish oil emulsion system. The mineral components of tropical seaweeds are dominated by calcium, potassium and sodium, as well as small amounts of copper, iron and zinc. A green alga usually contains epigallocatechin, gallocatechin, epigallocatechin gallate and catechin. However, catechin and its isomers are not found in some green and red algae. In the presence of a ferrous ion catalyst, all the methanol extracts from the seaweeds show significantly lower peroxide values of the emulsion than the control,and that of a green alga shows the strongest anti-oxidant activity. The highest chelation on ferrous ions is also found in the extract of this alga, which is significantly different from the other methanol extracts in both 3 and 24 h incubations.

  15. ANTIOXIDANT ACTIVITY AND PHARMACOLOGICAL SCREENING OF TINOSPORA CORDIFOLIA (THUNB.

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    M. P. SINHA

    2013-01-01

    Full Text Available The communication deals with the antioxidant activity of aqueous and methanolic extracts of Tinospora cordifolia(Thunb. stem, along with the phytochemical, pharmacological and nutritive properties. Methanolic extractshowed good antioxidant efficacy compare to the BHA standard. Reducing power of the extract showedconcentration dependence. Lipid peroxidation inhibition was not significant. Methanolic extract showed significantDPPH radical scavenging activity (EC50 = 0.5 mg/mL, while the superoxide radical scavenging activity of themethanolic extract was mild and showed concentration dependence. The phytochemical analysis showed lowamount of total ash content, i.e. 11.3 ± 1.4 mg/g. Total phenol, tannin and flavonoids contents were 17.3 ± 0.4,13.8 ± 0.5 and 6.5 ± 0.2 mg/g respectively which fall under moderate range. Crude fiber and moisture contents(231 ± 3.2 and 214 ± 5.3 mg/g respectively as well as carbohydrate content (184.1 ± 5.2 mg/g and nutritivevalue (1.46 ± 0.3 cal/g were significantly high. T. cordifolia stem showed high swelling and foaming indices,400 ± 3.5% and 111.12 ± 2.1% respectively, which is an indication of good drug release characteristics.Present findings suggest Tinospora cordifolia as potential source of natural antioxidant, fiber and nutrient contentin food, fodder and pharmaceutical industries.

  16. Chemical profiling and antioxidant activity of Bolivian propolis.

    Science.gov (United States)

    Nina, Nélida; Quispe, Cristina; Jiménez-Aspee, Felipe; Theoduloz, Cristina; Giménez, Alberto; Schmeda-Hirschmann, Guillermo

    2016-04-01

    Propolis is a relevant research subject worldwide. However, there is no information so far on Bolivian propolis. Ten propolis samples were collected from regions with high biodiversity in the main honey production places in Bolivia and were analyzed for their total phenolics (TP), flavonoids (TF) and antioxidant activity. The chemical profiles of the samples were assessed by TLC, HPLC-DAD, HPLC-DAD-MS/MS(n) and NMR analysis. TP, TF, TLC and NMR analysis showed significant chemical differences between the samples. Isolation of the main constituents by chromatography and identification by HPLC-DAD-MS/MS(n) achieved more than 35 constituents. According to their profiles, the Bolivian propolis can be classified into phenolic-rich and triterpene-rich samples. Propolis from the valleys (Cochabamba, Chuquisaca and Tarija) contained mainly prenylated phenylpropanoids, while samples from La Paz and Santa Cruz contained cycloartane and pentacyclic triterpenes. Phenolic-rich samples presented moderate to strong antioxidant activity while the triterpene-rich propolis were weakly active. High chemical diversity and differential antioxidant effects were found in Bolivian propolis. Our results provide additional evidence on the chemical composition and bioactivity of South American propolis. © 2015 Society of Chemical Industry.

  17. Mineral components and anti-oxidant activities of tropical seaweeds

    Science.gov (United States)

    Takeshi, Suzuki; Yumiko, Yoshie-Stark; Joko, Santoso

    2005-07-01

    Seaweeds are known to hold substances of high nutritional value; they are the richest resources of minerals important to the biochemical reactions in the human body. Seaweeds also hold non-nutrient compounds like dietary fiber and polyphenols. However, there is not enough information on the mineral compounds of tropical seaweeds. Also we are interested in the antioxidant activities of seaweeds, especially those in the tropical area. In this study, Indonesian green, brown and red algae were used as experimental materials with their mineral components analyzed by using an atomic absorption spectrophotometer. The catechins and flavonoids of these seaweeds were extracted with methanol and analyzed by high performance liquid chromatography (HPLC); the antioxidant activities of these seaweeds were evaluated in a fish oil emulsion system. The mineral components of tropical seaweeds are dominated by calcium, potassium and sodium, as well as small amounts of copper, iron and zinc. A green alga usually contains epigallocatechin, gallocatechin, epigallocatechin gallate and catechin. However, catechin and its isomers are not found in some green and red algae. In the presence of a ferrous ion catalyst, all the methanol extracts from the seaweeds show significantly lower peroxide values of the emulsion than the control, and that of a green alga shows the strongest antioxidant activity. The highest chelation on ferrous ions is also found in the extract of this alga, which is significantly different from the other methanol extracts in both 3 and 24 h incubations.

  18. Antioxidant Activity of Leaves and Fruits of Iranian Conifers

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    S. A. Emami

    2007-01-01

    Full Text Available Cupressus semipervirens var. horizontalis, Cupressus semipervirens var. semipervirens, Cupressus semipervirens cv. Cereifeormis, Juniperus communis subsp. hemisphaerica, Juniperus excelsa subsp. excelsa, Juniperus excelsa subsp. polycarpos, Juniperus foetidissima, Juniperus oblonga, Juniperus sabina, Platycladus orientalis and Taxus baccata are Iranian conifers. The antioxidant activity of leaves and fruits of these 11 different taxons were evaluated. The leaves of both male and female, and fruits of these plants were collected from different areas of the country. Methanol extract of leaves and fruits of these taxons were prepared. Antioxidant activity of each extracts was measured using two different tests of the ferric thiocyanate method and thiobarbituric acid. Results indicated that the methanol extracts of leaves, of male and female, and fruits of all these species (27 samples possessed antioxidant activity when tested with both methods. The antioxidant activity was then compared with those of α-tocopherol (a natural antioxidant and butylated hydroxytoluene (a synthetic antioxidant. Methanol extract of fruits of C. semipervirens cv. Cereifeormis showed the highest antioxidant activity while the methanol extract of leaves of C. semipervirens var. semipervirens possessed the lowest antioxidant activity. However, our finding showed that most of the tested extracts were showing strong antioxidant activity even higher than α-tocopherol.

  19. ANTIOXIDANT ACTIVITY OF WINTER SLAVONIAN GARLIC (Allium sativum L.)

    OpenAIRE

    2012-01-01

    Numerous studies conducted on garlic (Allium sativum L.), have proved the presence of antioxidants, phenolic compounds, sulfur compounds and several vitamins. The aim of this study was to investigate the differences in the content of total phenols, ascorbic acid; total antioxidant activities of four winter Slavonian garlic cultivars, as well as establishment of correlations between these parameters. The total antioxidant activity and the content of ascorbic acid and total phenols in four cult...

  20. Antioxidant activity of various extracts of selected gourd vegetables.

    Science.gov (United States)

    Yadav, Baljeet S; Yadav, Roshanlal; Yadav, Ritika B; Garg, Munish

    2016-04-01

    Study was conducted to evaluate the antioxidative activity of methanolic (ME), ethanolic (EE) and butanolic extracts (BE) of selected gourd vegetables. The antioxidant activity was investigated using different assays namely ferric thiocyanate test (FTC), thiobarbituric acid test (TBA), ferric reducing antioxidant power (FRAP) and DPPH free radicals scavenging test. A densitometric HPTLC analysis was performed for the analysis of phenolic acids and flavonoids. Different extracts of the selected gourd vegetables revealed different antioxidant activity. Different extracts of Lagenaria siceraria, Momordica charantia and Luffa cylindrica revealed significantly higher (p siceraria and M. charantia.

  1. Antioxidant and Antimicrobial Activities of Pogostemon cablin (Blanco Benth.

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    Bhanuz Dechayont

    2017-01-01

    Full Text Available Pogostemon cablin (Lamiaceae has been widely used in traditional medicine. In this study, the antioxidant and antimicrobial activities of leaves from P. cablin extracts were investigated. The water extracts had the highest total phenolic content 116.88±0.48 mg gallic acid equivalent/g of dry plant extract. Nevertheless, high levels of total flavonoid content were found in ethanolic extracts 280.12±2.04 mg quercetin equivalent/g of dry plant extract. The highest antioxidant activities were found for the ethanolic extract (IC50=18±0.90, 20±0.24 μg/mL by DPPH and ABTS scavenging assays, respectively. Both extracts showed moderate inhibition of superoxide inhibition (O2∙- and nitric oxide (NO production in concentration-dependent manner. Antibacterial activity was calculated by disk diffusion, minimum inhibitory concentration (MIC, and minimum bacterial concentration (MBC. The ethanolic extract had the greatest activity against methicillin resistant Staphylococcus aureus, methicillin sensitive S. aureus, and Streptococcus pyogenes with zone diameters of 11.67±1.53, 10.33±2.52, and 10.33±1.15 mm, respectively. The corresponding MIC and MBCs were 5, 0.625, and 0.039 mg/mL. P. cablin extracts contain antioxidant and antibacterial properties that should be exploited for possible clinical application.

  2. Benzofuranyl Esters: Synthesis, Crystal Structure Determination, Antimicrobial and Antioxidant Activities

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    C. S. Chidan Kumar

    2015-09-01

    Full Text Available A series of five new 2‐(1‐benzofuran‐2‐yl‐2‐oxoethyl 4-(un/substitutedbenzoates 4(a–e, with the general formula of C8H5O(C=OCH2O(C=OC6H4X, X = H, Cl, CH3, OCH3 or NO2, was synthesized in high purity and good yield under mild conditions. The synthesized products 4(a–e were characterized by FTIR, 1H-, 13C- and 1H-13C HMQC NMR spectroscopic analysis and their 3D structures were confirmed by single-crystal X-ray diffraction studies. These compounds were screened for their antimicrobial and antioxidant activities. The tested compounds showed antimicrobial ability in the order of 4b < 4a < 4c < 4d < 4e and the highest potency with minimum inhibition concentration (MIC value of 125 µg/mL was observed for 4e. The results of antioxidant activities revealed the highest activity for compound 4e (32.62% ± 1.34% in diphenyl-2-picrylhydrazyl (DPPH radical scavenging, 4d (31.01% ± 4.35% in ferric reducing antioxidant power (FRAP assay and 4a (27.11% ± 1.06% in metal chelating (MC activity.

  3. Polyphenol content and antioxidant activity of fourteen wild edible fruits from Burkina Faso.

    Science.gov (United States)

    Lamien-Meda, Aline; Lamien, Charles Euloge; Compaoré, Moussa M Y; Meda, Roland N T; Kiendrebeogo, Martin; Zeba, Boukare; Millogo, Jeanne F; Nacoulma, Odile G

    2008-03-06

    A total of fourteen (14) species of wild edible fruits from Burkina Faso were analyzed for their phenolic and flavonoid contents, and their antioxidant activities using the DPPH, FRAP and ABTS methods. The data obtained show that the total phenolic and total flavonoid levels were significantly higher in the acetone than in the methanol extracts.Detarium microcarpum fruit had the highest phenolic and the highest flavonoid content,followed by that of Adansonia digitata, Ziziphus mauritiana, Ximenia americana and Lannea microcarpa. Significant amounts of total phenolics were also detected in the other fruit species in the following order of decreasing levels: Tamarindus indica > Sclerocaryabirrea > Dialium guineense > Gardenia erubescens > Diospyros mespiliformis > Parkiabiglobosa > Ficus sycomorus > Vitellaria paradoxa. Detarium microcarpum fruit also showed the highest antioxidant activity using the three antioxidant assays. Fruits with high antioxidant activities were also found to possess high phenolic and flavonoid contents. There was a strong correlation between total phenolic and flavonoid levels and antioxidant activities.

  4. Analysis of antioxidant activity and total phenolic content of some homoeopathic mother tinctures

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    Saeed Ahmad

    2017-01-01

    Full Text Available Background: Antioxidant compounds are widely used in health protection. Homoeopathic mother tinctures are commonly prescribed for prevention and cure of many illnesses. Objective: The present study focused to determine the antioxidant potential of six commonly prescribed mother tinctures. i.e. Syzygium jambolanum, Damiana, Cinchona officinalis, Chelidonium majus, Convallaria majalis, Coca. Materials and Methods: Antioxidant activity was estimated by 2,2-diphenyl-1-picrylhydrazyl (DPPH inhibition assay. A volume of 0.1 mM solution of DPPH was used while mother tinctures (5, 2.5, and 1.25 μl volumes were used for estimating antioxidant activity. Quercetin was taken as a standard control in antioxidant activity. Total phenolic content was measured by Folin–Ciocalteu reagent assay. Total phenolic content of mother tinctures was measured in comparison to gallic acid. Results: Results of the study showed the significant antioxidant activity and high total phenolic content of all tested mother tinctures in the following order: Syzygium jambolanum, Damiana, Cinchona officinalis, Chelidonium majus, Convallaria majalis, Coca. Conclusion: Antioxidant potential of mother tinctures is related to total phenolic content present in them, and their role in prevention and cure of diseases may link through their antioxidant activity. Among six selected mother tinctures, S. jambolanum has highest antioxidant potential.

  5. PHYTOCHEMICAL STUDY, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF EUPHORBIA RESINIFERA L.

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    Houcine Benmehdi

    2013-09-01

    Full Text Available The present study was aimed at detecting the phytochemicals and evaluating the antioxidant and antimicrobial activities of Euphorbia resinifera known for their medicinal properties in folk medicine. Phytochemical screening was carried out on the aerial part of Euphorbia resinifera. The assessment of antifungal activity was performed in terms of percentage of radial growth on solid medium (potatoes dextrose agar PDA against Aspergillus flavus and Penicillium expansum. The antibacterial effect was studied by the agar direct contact method using Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli strains. Then, the antioxidant evaluation of the extracts of alkaloids, flavonoids and methanolic extract was performed by DPPH• free radical scavenging and high performance thin layer chromatography (HPTLC techniques. The phytochemical estimation revealed the presence of alkaloids, flavonoids and saponosides. These phytochemicals were isolated from the plant with yields of 0.7 %, 0.4 % and 0.35 %. HPTLC screening provided qualitatively the antioxidant effect of extracts under study. Furthermore, it was found that the methanolic, flavonoids and alkaloids extracts had a potent DPPH scavenging potency with IC50 values of 0.0086; 0.378 and 1.171 mg/mL, respectively. Finally, the results of antimicrobial activity of the aqueous extract showed a pronounced antifungal activity against the tested strains. The percentage inhibition values were found to be in the range of 64.14 to 85.51 % against Aspergillus flavus and 60.33 to 92.28 % against Penicillium expansum. In contrast, the same extract inhibited only the growth of Escherichia coli bacteria. Further study is recommended to isolate and elucidate the active compounds and to evaluate in vivo their antioxidant and antimicrobial effect.

  6. Cooking techniques improve the levels of bioactive compounds and antioxidant activity in kale and red cabbage.

    Science.gov (United States)

    Murador, Daniella Carisa; Mercadante, Adriana Zerlotti; de Rosso, Veridiana Vera

    2016-04-01

    The aim of this study is to investigate the effects of different home cooking techniques (boiling, steaming, and stir-frying) in kale and red cabbage, on the levels of bioactive compounds (carotenoids, anthocyanins and phenolic compounds) determined by high-performance liquid chromatography coupled with photodiode array and mass spectrometry detectors (HPLC-DAD-MS(n)), and on the antioxidant activity evaluated by ABTS, ORAC and cellular antioxidant activity (CAA) assays. The steaming technique resulted in a significant increase in phenolic content in kale (86.1%; pkale, steaming resulted in significant increases in antioxidant activity levels in all of the evaluation methods. In the red cabbage, boiling resulted in a significant increase in antioxidant activity using the ABTS assay but resulted in a significant decrease using the ORAC assay. According to the CAA assay, the stir-fried sample displayed the highest levels of antioxidant activity.

  7. Ultrasound extraction of polysaccharides from mulberry leaves and their effect on enhancing antioxidant activity.

    Science.gov (United States)

    Zhang, Dong-Yang; Wan, Yi; Xu, Jian-Yi; Wu, Guo-Hua; Li, Long; Yao, Xiao-Hui

    2016-02-10

    A Box-Behnken design (BBD) was applied to optimize the ultrasound-assisted extraction of polysaccharides from mulberry leaves. Under the optimum conditions of an extraction temperature of 57 °C, an extraction time of 80 min and a liquid/solid ratio of 53 mL/g, the mulberry leaf polysaccharide (MLP) yield was 6.92 ± 0.29%. Then, three fractions of MLPs were obtained by deproteinization, dialysis and decolorization. The carbohydrate content, FT-IR spectrum and monosaccharide composition of the MLPs were also investigated. The antioxidant activities of the three fractions were compared, and the results indicated that the antioxidant activities decreased with the increasing MLP purity. Therefore, highly concentrated MLPs were shown to have very little antioxidant activity. After quercetin (10 μg/mL) was added, the antioxidant activities were improved significantly. This result showed that MLPs and quercetin have a synergistic effect on the antioxidant activity. Although the MLPs have very little antioxidant activity alone, they greatly enhance the antioxidant activity of flavonoids. Thus, MLPs can be used as an antioxidant activity enhancer in the food industry.

  8. Stability and Antioxidant Activity of Semi-synthetic Derivatives of 4-Nerolidylcatechol

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    Emerson Silva Lima

    2012-12-01

    Full Text Available 4-nerolidylcatechol (4-NC is an unstable natural product that exhibits important antioxidant, anti-inflammatory and other properties. It is readily obtainable on a multi-gram scale through straightforward solvent extraction of the roots of cultivated Piper peltatum or P. umbellatum, followed by column chromatography on the resulting extract. Semi-synthetic derivatives of 4-NC with one or two substituent groups (methyl, acetyl, benzyl, benzoyl on the O atoms have been introduced that have increased stability compared to 4-NC and significant in vitro inhibitory activity against the human malaria parasite Plasmodium falciparum. Antioxidant and anti-inflammatory properties may be important for the antiplasmodial mode of action of 4-NC derivatives. Thus, we decided to investigate the antioxidant properties, cytotoxicity and stability of 4-NC derivatives as a means to explore the potential utility of these compounds. 4-NC showed high antioxidant activity in the DPPH and ABTS assays and in 3T3-L1 cells (mouse embryonic fibroblast, however 4-NC was more cytotoxic (IC50 = 31.4 µM and more unstable than its derivatives and lost more than 80% of its antioxidant activity upon storage in solution at −20 °C for 30 days. DMSO solutions of mono-O-substituted derivatives of 4-NC exhibited antioxidant activity and radical scavenging activity in the DPPH and ABTS assays that was comparable to that of BHA and BHT. In the cell-based antioxidant model, most DMSO solutions of derivatives of 4-NC were less active on day 1 than 4-NC, quercetin and BHA and more active antioxidants than BHT. After storage for 30 days at −20 °C, DMSO solutions of most of the derivatives of 4-NC were more stable and exhibited more antioxidant activity than 4-NC, quercetin and BHA and exhibited comparable antioxidant activity to BHT. These findings point to the potential of derivatives of 4-NC as antioxidant compounds.

  9. Antioxidant Activity of Mulberry Fruit Extracts

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    Ryszard Amarowicz

    2012-02-01

    Full Text Available Phenolic compounds were extracted from the fruits of Morus nigra and Morus alba using methanol and acetone. The sugar-free extracts (SFEs were prepared using Amberlite XAD-16 column chromatography. All of the SFEs exhibited antioxidant potential as determined by ABTS (0.75–1.25 mmol Trolox/g, DPPH (2,2-diphenyl-1-picrylhydrazyl (EC50 from 48 μg/mL to 79 μg/mL, and reducing power assays. However, a stronger activity was noted for the SFEs obtained from Morus nigra fruits. These extracts also possessed the highest contents of total phenolics: 164 mg/g (methanolic SFE and 173 mg/g (acetonic SFE. The presence of phenolic acids and flavonoids in the extracts was confirmed using HPLC method and chlorogenic acid and rutin were found as the dominant phenolic constituents in the SFEs.

  10. Antioxidant activity of mulberry fruit extracts.

    Science.gov (United States)

    Arfan, Muhammad; Khan, Rasool; Rybarczyk, Anna; Amarowicz, Ryszard

    2012-01-01

    Phenolic compounds were extracted from the fruits of Morus nigra and Morus alba using methanol and acetone. The sugar-free extracts (SFEs) were prepared using Amberlite XAD-16 column chromatography. All of the SFEs exhibited antioxidant potential as determined by ABTS (0.75-1.25 mmol Trolox/g), DPPH (2,2-diphenyl-1-picrylhydrazyl) (EC(50) from 48 μg/mL to 79 μg/mL), and reducing power assays. However, a stronger activity was noted for the SFEs obtained from Morus nigra fruits. These extracts also possessed the highest contents of total phenolics: 164 mg/g (methanolic SFE) and 173 mg/g (acetonic SFE). The presence of phenolic acids and flavonoids in the extracts was confirmed using HPLC method and chlorogenic acid and rutin were found as the dominant phenolic constituents in the SFEs.

  11. Polyphenol profile and antioxidant activity of extracts from olive leaves

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    Svetla Yancheva

    2016-02-01

    Full Text Available The compounds, derivatives of olive leaves have a high antioxidant activity. The content of the total phenolic compounds (TPC, antioxidant activity (AOA and HPLC polyphenol profile of methanol extracts from the leaves of the olive cultivars Chondrolia Halkidiki, Kalamon, Koroneiki grown in the nursery (in vivo and in vitro plants of Chondrolia Halkidiki were compared. The results obtained for TPC varied between 9.2±0.5 mgGAE*gDW-1 and 16.4±0.5 mgGAE*gDW-1 . Antioxidant capacity was determined by four methods DPPH, ABTS, FRAP and CUPRAC. The highest results for TPC and AOA were achieved for the leaves of Chondrolia Halkidiki grown in vitro. A high correlation between the results gained from the TPC and AOA was established. Conducted HPLC analysis revealed the presence of 3,4- dihydroxybenzoic, caffeic, sinapic and ferulic acids and quercetin, hesperidin and luteolin and the quercetin glycosides rutin and hyperoside.

  12. Evaluating Antiproliferative and Antioxidant Activity of Marrubium crassidens

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    Sanaz Hamedeyazdan

    2014-10-01

    Full Text Available Purpose: Naturally occurring substances as novel drugs in cancer therapy, at all times, represent a challenge to science since medicinal plants are proving to be brilliant sources of new chemopreventive agents. Methods: In the present study, methanol extract from aerial parts of Marrubium crassidens was assessed for its antiproliferative activity in the breast cancer cell line MCF-7 through MTT bioassay using cell viability and cytotoxicity indices. The antioxidant property of M. crassidens extract together with its phenolic and flavonoids content were evaluated, as well. Results: According to data obtained in the study, M. crassidens exhibited antiproliferative activity with a gradual rise in cytotoxicty effect setting out on 240μg/mL concentration of the extract. Moreover, the RC50 value for antioxidant activity of the extract was determined as 40μg/mL and values for the total phenolic and flavonoids were calculated as 512.64mg gallic acid equivalent and 212.73mg quercetin equivalent per 100g of dry plant material. Conclusion: Generally, the observed antiproliferative and antioxidant properties of M. crassidens could be certified to the high amounts of phenolic and flavonoid content detected in the extract.

  13. Physical activity alters antioxidant status in exercising elderly subjects.

    Science.gov (United States)

    Rousseau, Anne-Sophie; Margaritis, Irène; Arnaud, Josiane; Faure, Henri; Roussel, Anne-Marie

    2006-07-01

    Nutritional adequacy and physical activity are two aspects of a health-promoting lifestyle. Not much is known about antioxidant nutrient requirements for exercising elderly (EE) subjects. The question of whether exercise training alters the status of antioxidant vitamins as well as trace elements in elderly subjects and fails to balance the age-related increase in oxidative stress is addressed in this study. There were 18 EE (68.1+/-3.1 years), 7 sedentary elderly (SE; 70.4+/-5.0 years), 17 exercising young (EY; 31.2+/-7.1 years) and 8 sedentary young (SY; 27.1+/-5.8 years) subjects who completed 7-day food and activity records. Each subject's blood was sampled on Day 8. A similar selenium (Se) status but a higher erythrocyte glutathione peroxidase (GSH-Px) activity were found in EE subjects as compared with EY and SE subjects. Blood oxidized glutathione was higher and plasma total thiol was lower in EE subjects as compared with EY subjects. Mean vitamin C (167 vs. 106 mg/day), vitamin E (11.7 vs. 8.3 mg/day) and beta-carotene (4 vs. 2.4 mg/day) intakes were higher in EE subjects as compared with EY subjects. However, EE subjects exhibited the lowest plasma carotenoid concentrations, especially in beta-carotene, which was not related to intakes. Despite high intakes of antioxidant micronutrients, no adaptive mechanism able to counteract the increased oxidative stress in aging was found in EE subjects. Results on GSH-Px activity illustrate that the nature of the regulation of this biomarker of Se status is different in response to training and aging. These data also strongly suggest specific antioxidant requirements for athletes with advancing age, with a special attention to carotenoids.

  14. The study of antioxidant activity of multicomponent vegetable puree

    Directory of Open Access Journals (Sweden)

    A. V. Trushechkin

    2013-01-01

    Full Text Available Studied the antioxidant activity of multicomponent vegetable puree, consisting of 20 % of eggplant, 20 % of zucchini, 16 % of bell peppers, 15 % of tomatoes, 14 % of carrots, 10 % of onions and 5 % of garlic. These results showed an increase in concentration of the antioxidant activity of puree and, consequently, increase its quality.

  15. Application of KRL test to assess total antioxidant activity in pigs: sensitivity to dietary antioxidants.

    Science.gov (United States)

    Rossi, Raffaella; Pastorelli, Grazia; Corino, Carlo

    2013-04-01

    The application of Kit Radicaux Libres (KRL) test to assess total blood antioxidant activity in pigs was evaluated. The KRL has been validated and is widely used in humans for assessing the effectiveness of natural or pharmaceutical treatments, and in vitro to evaluate the antioxidant activities of natural or synthetic antioxidants. In this study the sensitivity of the KRL test in assessing the effectiveness of dietary antioxidant supplementation (vitamin E and plant extract) was evaluated in two different phases of pig breeding. The first trial, in post-weaned piglets (40 piglets/group) fed dietary vitamin E supplementation for 60 days, indicated that there was a higher total antioxidant activity (P=0.032) of whole blood and of red blood cells (P=0.001) than for control pigs. The second trial indicated that long-term supplementation of water soluble plant extract (20 pigs/group) from the leaves of Verbenaceae (Lippia spp.) tended (P=0.091) to increase antioxidant activity in the whole blood of treated, rather than control pigs. These results indicate that the KRL might be recommended as one of efficient means for evaluating antioxidant activity of dietary ingredients fed to pigs.

  16. Antioxidant activity of carbocysteine lysine salt monohydrate.

    Science.gov (United States)

    Pinamonti, S; Venturoli, L; Leis, M; Chicca, M; Barbieri, A; Sostero, S; Ravenna, F; Daffonchio, L; Novellini, R; Ciaccia, A

    2001-09-01

    Reactive oxygen radicals are involved in many respiratory diseases, including chronic obstructive pulmonary disease (COPD). Carbocysteine lysine salt monohydrate (CLS) is a mucoactive drug effective in the treatment of bronchopulmonary diseases characterized by mucus alterations, including COPD. In the present study, the antioxidant activity of CLS was studied in vitro in three different oxygen radical producing systems, i.e. bronchoalveolar lavages (BAL) from patients affected by COPD, ultrasound treated human serum and cultured human lung endothelial cells challenged with elastase. BAL, exposed or not to different concentrations of CLS (1.5-30 mM), was assayed for free radical content by fluorometric analysis of DNA unwinding (FADU) or by cytochrome c reduction kinetics. Human serum was treated with ultrasound in the presence or absence of CLS (1.5, 2.5 mM) or N-acetyl cysteine (NAC; 4, 5 mM) and assayed for free radical content by FADU. Human endothelial cells cultured in vitro from pulmonary artery were incubated with elastase (0.3 IU/mL), in the presence or absence of glutathione (GSH; 0.65 mM) or CLS (0.16 mM). The supernatant was tested for cytochrome c reduction kinetics whereas cell homogenates were assessed for xanthine oxidase (XO) content by SDS-PAGE. Results showed that CLS is more effective as an in vitro scavenger in comparison to GSH and NAC. CLS reduced the damage of DNA from healthy donors exposed to COPD-BAL and was able to quench clastogenic activity induced in human serum by exposure to ultrasound at concentrations as low as 2.5 mM. NAC protect DNA from radical damage, starting from 5 mM. In human lung endothelial cells cultured in presence of elastase, CLS (0.16 mM) decreased xanthine oxidase activity. These results suggest that CLS could act by interfering with the conversion of xanthine dehydrogenase into superoxide-producing xanthine oxidase. The antioxidant activity of CLS could contribute to its therapeutic activity by reducing radical

  17. Phytotoxic and antioxidant activity of seven native fruits of Brazil

    Directory of Open Access Journals (Sweden)

    Marize Terezinha Lopes Pereira Peres

    2013-12-01

    Full Text Available We investigated antioxidant capacity, phenolic content and phytotoxic effects in seven species of fruit native to Brazil: Acrocomia aculeata, Hymenaea stigonocarpa, Tamarindus indica, Byrsonima intermedia, Psidium firmum, Myrciaria cauliflora and Ficus obtusifolia. Phytotoxic effects were assessed from crude ethanol extracts and semipurified fractions. In four of the seven fruits, total phenolic content, as determined by Folin-Ciocalteu assay, was higher in the peel than in the pulp. We found that total phenolic content correlated significantly with antioxidant capacity. High-performance liquid chromatography with diode array detection revealed the presence of the flavonoids quercetin and rutin in some fruits. We also evaluated the allelopathic effects of the crude ethanol extract and semipurified fractions. In most of the essays, the ethyl acetate fractions showed the highest level of activity.

  18. Evaluation of diverse antioxidant activities of Galium aparine

    Science.gov (United States)

    Bokhari, Jasia; Khan, Muhammad R.; Shabbir, Maria; Rashid, Umbreen; Jan, Shumaila; Zai, Jawaid A.

    2013-02-01

    Methanol extract and its n-hexane, ethyl acetate, butanol and aqueous fraction of Galium aparine L. (Rubiacea) were evaluated in vitro for their antioxidant capacity (DPPH, superoxide radical, phosphomolybdate assay); reducing power (ABTS, hydroxyl, hydrogen peroxide, to reduce Fe3+ to Fe2+ ions) and to estimate total flavonoid and phenolic contents. All the free radical generating assay models depicted differential positive scavenging activity but considerable magnitude for all the fractions. The results showed that aqueous fraction strongly scavenge the DPPH, ABTS, hydroxyl, hydrogen peroxide and superoxide radicals. A significantly high correlation coefficient existed between IC50 values of DPPH and superoxide radical with total phenolic content and phosphomolybdate assay with total flavonoid contents, respectively. These results suggested that aqueous fraction can be a good source of antioxidant therapeutic in oxidative stress damages.

  19. Antioxidant activity of some Moroccan marine microalgae: Pufa profiles, carotenoids and phenolic content.

    Science.gov (United States)

    Maadane, Amal; Merghoub, Nawal; Ainane, Tarik; El Arroussi, Hicham; Benhima, Redouane; Amzazi, Saaid; Bakri, Youssef; Wahby, Imane

    2015-12-10

    In order to promote Moroccan natural resources, this study aims to evaluate the potential of microalgae isolated from Moroccan coastlines, as new source of natural antioxidants. Different extracts (ethanolic, ethanol/water and aqueous) obtained from 9 microalgae strains were screened for their in vitro antioxidant activity using DPPH free radical-scavenging assay. The highest antioxidant potentials were obtained in Dunalliela sp., Tetraselmis sp. and Nannochloropsis gaditana extracts. The obtained results indicate that ethanol extract of all microalgae strains exhibit higher antioxidant activity, when compared to water and ethanol/water extracts. Therefore, total phenolic and carotenoid content measurement were performed in active ethanol extracts. The PUFA profiles of ethanol extracts were also determined by GC/MS analysis. The studied microalgae strains displayed high PUFA content ranging from 12.9 to 76.9 %, total carotenoids content varied from 1.9 and 10.8mg/g of extract and total polyphenol content varied from 8.1 to 32.0mg Gallic acid Equivalent/g of extract weight. The correlation between the antioxidant capacities and the phenolic content and the carotenoids content were found to be insignificant, indicating that these compounds might not be major contributor to the antioxidant activity of these microalgae. The microalgae extracts exerting the high antioxidant activity are potential new source of natural antioxidants.

  20. Phytochemical Analysis and Antioxidant Activity of Salvia chloroleuca Aerial Extracts

    Science.gov (United States)

    Salimikia, Iraj; Reza Monsef-Esfahani, Hamid; Gohari, Ahmad Reza; Salek, Mehrnoosh

    2016-01-01

    Background Salvia, known as Maryam Goli in the Persian language, is an important genus that includes approximately 900 species in the Lamiaceae family. There are 58 Salvia species growing naturally in Iran, including Salvia chloroleuca Rech. f. and Allen., which grows wild in the northeastern and central parts of the country. Objectives This study was designed to determine the chemical composition, in vitro antioxidant activity, and total phenol content of various extracts of S. chloroleuca. Materials and Methods Dried aerial parts of the plant were crushed, then sequentially extracted with n-hexane, ethyl acetate, and methanol. The fractions of S. chloroleuca were subjected to silica gel column chromatography and Sephedex LH-20. The antioxidant activities of these extracts were measured by ferric reducing antioxidant power (FRAP), and the total phenolic contents of the extracts were evaluated using Folin-Ciocalteu reagent. Results The separation and purification processes were carried out using different chromatographic methods. Structural elucidation was on the basis 1H-NMR and 13C-NMR spectral data, in comparison with that reported in the literature. The isolated compounds were salvigenin (1), luteolin (2), cirsiliol (3), β-sitosterol (4), and daucosterol (5). Ethyl acetate extract displayed the highest level of total antioxidants and total polyphenols compared to the other analyzed extracts (n-hexane and methanol). In the FRAP assay, ethyl acetate extract had the highest (230.4±10.5) FRAP value, followed by methanol (211.4 ± 8.3) and n-hexane (143.4 ± 12.04). Total phenol contents were calculated to be 13.8 ± 0.3, 58.25 ± 0.05, and 43.48 ± 0.38 mg of gallic acid/100 g in the n-hexane, ethyl acetate, and methanol extracts, respectively. Conclusions The above-mentioned compounds were isolated for the first time from S. chloroleuca. The antioxidant activity of this plant could be in part related to isolated flavonoids and sterols. The results of this study

  1. Acute Exercise Increases Plasma Total Antioxidant Status and Antioxidant Enzyme Activities in Untrained Men

    Directory of Open Access Journals (Sweden)

    C. Berzosa

    2011-01-01

    Full Text Available Antioxidant defences are essential for cellular redox regulation. Since free-radical production may be enhanced by physical activity, herein, we evaluated the effect of acute exercise on total antioxidant status (TAS and the plasma activities of catalase, glutathione reductase, glutathione peroxidase, and superoxide dismutase and its possible relation to oxidative stress resulting from exercise. Healthy untrained male subjects (=34 performed three cycloergometric tests, including maximal and submaximal episodes. Venous blood samples were collected before and immediately after each different exercise. TAS and enzyme activities were assessed by spectrophotometry. An increase of the antioxidant enzyme activities in plasma was detected after both maximal and submaximal exercise periods. Moreover, under our experimental conditions, exercise also led to an augmentation of TAS levels. These findings are consistent with the idea that acute exercise may play a beneficial role because of its ability to increase antioxidant defense mechanisms through a redox sensitive pathway.

  2. Studies on antioxidant activity of teasaponins after hydrolyzed by enzyme

    Science.gov (United States)

    Tian, Jing; Zhao, Sen; Xu, Longquan; Fei, Xu; Wang, Xiuying; Wang, Yi

    The biological activity of teasaponins and their molecular structure are closely related, and the activity of saponins may be increased with the change of their molecular structure. In this report, teasaponins were hydrolyzed by Aspergillus niger for increasing the antioxidant activity. The antioxidant activity of teasaponins before and after hydrolyzed was tested by DPPH, and the result showed four new teasaponins were produced after hydrolysis, and their antioxidant activity was increased significantly than the original teasaponins before hydrolysis, the radical scavenging capacity (RSC) was partly up to 95 %.

  3. Study on the antioxidant activity and membrane interaction of a multiple antioxidant

    DEFF Research Database (Denmark)

    Jodko-Jodko-Piórecka, Kasia; Klösgen, Beate; Litwinienko, Grzegorz

    Catecholamines, including dopamine, have a role in the transduction of nervous stimuli. Apart from that, they might behave as endogenous phenolic antioxidants protecting the neuronal tissue from deleterious effects of oxidative stress. Results from our preliminary study1 even indicate a synergistic...... effect: an interplay of catecholamines with other molecules seems to enhance their antioxidant activity (e.g. 3,4-dihydroxyphenylalanine - L-DOPA -, and 2,2,5,7,8-pentamethyl-6-hydroxychroman - PMHC -, an analogue of α-tocopherol). Addition of small amounts of catecholamines suppresses or largely...... inhibits the peroxidation of lipids2. The understanding of the mechanism of antioxidant action of catecholamines and their interplay with other antioxidants in lipid membranes requires interdisciplinary research on the kinetics and thermodynamics of antioxidant(s)/membrane interactions. Here we report...

  4. Thai Fruits Exhibit Antioxidant Activity and Induction of Antioxidant Enzymes in HEK-293 Cells

    Directory of Open Access Journals (Sweden)

    Natthinee Anantachoke

    2016-01-01

    Full Text Available The cellular antioxidant enzymes play the important role of protecting the cells and organisms from the oxidative damage. Natural antioxidants contained in fruits have attracted considerable interest because of their presumed safety and potential nutritional value. Even though antioxidant activities of many fruits have been reported, the effects of phytochemicals contained in fruits on the induction of antioxidant enzymes in the cells have not been fully defined. In this study, we showed that extracts from Antidesma ghaesembilla, Averrhoa bilimbi, Malpighia glabra, Mangifera indica, Sandoricum koetjape, Syzygium malaccense, and Ziziphus jujuba inhibited H2O2-induced intracellular reactive oxygen species production in HEK-293 cells. Additionally, these Thai fruit extracts increased the mRNA and protein expressions of antioxidant enzymes, catalase, glutathione peroxidase-1, and manganese superoxide dismutase. The consumption of Thai fruits rich in phenolic compounds may reduce the risk of oxidative stress.

  5. Thai Fruits Exhibit Antioxidant Activity and Induction of Antioxidant Enzymes in HEK-293 Cells

    Science.gov (United States)

    Anantachoke, Natthinee; Lomarat, Pattamapan; Praserttirachai, Wasin; Khammanit, Ruksinee

    2016-01-01

    The cellular antioxidant enzymes play the important role of protecting the cells and organisms from the oxidative damage. Natural antioxidants contained in fruits have attracted considerable interest because of their presumed safety and potential nutritional value. Even though antioxidant activities of many fruits have been reported, the effects of phytochemicals contained in fruits on the induction of antioxidant enzymes in the cells have not been fully defined. In this study, we showed that extracts from Antidesma ghaesembilla, Averrhoa bilimbi, Malpighia glabra, Mangifera indica, Sandoricum koetjape, Syzygium malaccense, and Ziziphus jujuba inhibited H2O2-induced intracellular reactive oxygen species production in HEK-293 cells. Additionally, these Thai fruit extracts increased the mRNA and protein expressions of antioxidant enzymes, catalase, glutathione peroxidase-1, and manganese superoxide dismutase. The consumption of Thai fruits rich in phenolic compounds may reduce the risk of oxidative stress. PMID:28074103

  6. Polysaccharides in Sipunculus nudus: Extraction condition optimization and antioxidant activities

    Science.gov (United States)

    Zhang, Qin; Dong, Lanfang; Tong, Tong; Wang, Qingchao; Xu, Mingzhu

    2017-02-01

    Marine organisms constitute unlimited resource of bioactive substances due to their high biodiversity and represent a valuable source of new compounds. This study optimized the alkali-extraction conditions and antioxidant activities of soluble polysaccharides from the body wall of Sipunculus nudus. The effects of solid-liquid ratio, extraction duration, extraction temperature, and alkali concentration on the yield of S. nudus polysaccharides (SNP) were examined, according to which the optimal combination of extraction parameters was obtained by an orthogonal test. The relative influencing importance of different extraction parameters on the yield of SNP followed the order as solid-liquid ratio > extraction temperature > alkali concentration > extraction duration. The highest extraction yield, 1.98%, was achieved under an optimal extraction condition: temperature 60°C, solid-liquid ratio 1:6 g mL-1, duration 5 h, and alkali (NaOH) mass fraction 6%. The in vitro antioxidant activities examination showed that extracted SNP under this optimized condition had strong power in reducing certain hydroxyl and superoxide radical scavenging abilities. The promising results showed that extracted SNP could be a potent natural antioxidant.

  7. Functional composition and antioxidant activities of eight Moroccan date frui

    Directory of Open Access Journals (Sweden)

    Eimad dine Tariq Bouhlali

    2017-07-01

    Full Text Available The aim of this study was to determine the functional composition and antioxidant activities of eight major date fruit varieties grown in Morocco. The analysis shows that date fruit contains a high amount of sugar (66.03–83.05% DW but a low content of fat (0.218–0.363% DW and protein (2.2–3.45% DW. Among the eight studied minerals potassium, calcium and magnesium were the predominant. Moreover, the niacin is the major B vitamin of all analyzed varieties. The total phenolic content was found between 331.86 and 537.07 mg GAE/100 g DW, the flavonoid between 68.88 and 208.53 mg of RE/100 g DW and condensed tannins between 57.56 and 92.141 mg CE/100 g DW, the antioxidant activity ranged between 383.90 and 846.94 μmol TE/100 g DW for ABTS, 6.255 and 2.046 g of date/l for DPPHIC50 and 406.614 and 860.89 μmol TE/100 g DW for FRAP assays. The results suggest that date fruit, which is good source of vital nutrients and antioxidant, is an extensive and varied field.

  8. Acute Exercise Increases Plasma Total Antioxidant Status and Antioxidant Enzyme Activities in Untrained Men

    OpenAIRE

    Berzosa, C.; I. Cebrián; Fuentes-Broto, L.; E. Gómez-Trullén; Piedrafita, E.; Martínez-Ballarín, E.; López-Pingarrón, L.; Reiter, R. J.; García, J. J.

    2011-01-01

    Antioxidant defences are essential for cellular redox regulation. Since free-radical production may be enhanced by physical activity, herein, we evaluated the effect of acute exercise on total antioxidant status (TAS) and the plasma activities of catalase, glutathione reductase, glutathione peroxidase, and superoxide dismutase and its possible relation to oxidative stress resulting from exercise. Healthy untrained male subjects ( = 3 4 ) performed three cycloergometric tests, including maxi...

  9. Study of Stevia rebaudiana Bertoni antioxidant activities and cellular properties.

    Science.gov (United States)

    Bender, Cecilia; Graziano, Sara; Zimmermann, Benno F

    2015-01-01

    The aim of our study was to determine the antioxidant activities, cytotoxicity and proliferative properties in Stevia rebaudiana leaves and stems. Leaves extracts exhibited a higher antioxidant activity than stems extract, through oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity (CAA) assays. Stevioside and rebaudioside A, the main sweetening metabolites in stevia leaves, exhibited a low ORAC value in comparison with plant extracts, while did not elicit any CAA. Stevia rebaudiana did not exhibit toxicity against HepG2 (hepatocellular carcinoma) human cells. No proliferative nor catalase modulations were observed in cells treated with such extracts. Our findings support the promising role of stevia that, apart from its sweetness, can act as a source of antioxidants, even at the intracellular level. This activity makes S. rebaudiana crude extract an interesting resource of natural sweetness with antioxidant properties which may find numerous applications in foods and nutritional supplements industries.

  10. Relationship between Estradiol and Antioxidant Enzymes Activity of Ischemic Stroke

    Directory of Open Access Journals (Sweden)

    Nasrin Sheikh

    2009-01-01

    Full Text Available Some evidence suggests the neuroprotection of estrogen provided by the antioxidant activity of this compound. The main objective of this study was to determine the level of estradiol and its correlation with the activity of antioxidant enzymes, total antioxidant status and ferritin from ischemic stroke subjects. The study population consisted of 30 patients with acute ischemic stroke and 30 controls. There was no significant difference between estradiol in stroke and control group. The activity of superoxide dismutase and level of ferritin was higher in stroke compared with control group (<.05, <.001, resp.. There was no significant correlation between estradiol and glutathione peroxidase, glutathione reductase, catalase, total antioxidant status, and ferritin in stroke and control groups. We observed inverse correlation between estradiol with superoxide dismutase in males of stroke patients (=−0.54, =.029. Our results supported that endogenous estradiol of elderly men and women of stroke or control group has no antioxidant activity.

  11. Bio-antioxidants - a chemical base of their antioxidant activity and beneficial effect on human health.

    Science.gov (United States)

    Kancheva, V D; Kasaikina, O T

    2013-01-01

    The paradox of aerobic life is that higher eukaryotic organisms cannot exist without oxygen, yet oxygen is inherently dangerous to their existence. Autoxidation of organic substances frequently occurs via free radical mechanism which generates different active radicals and peroxides OH(•), O2 (•-), LO2 (•), HOOH, LOOH, so called reactive oxygen species (ROS), which appear to be responsible for oxygen toxicity. To survive in such an unfriendly oxygen environment, living organisms generate - or obtain from food - a variety of water- and lipid-soluble antioxidant compounds. Biologically active compounds with antioxidant potential, i.e. bio-antioxidants (natural and their synthetic analogues) have a wide range of applications. They are important drugs, antibiotics, agrochemical substitutes, and food preservatives. Many of the drugs today are synthetic modifications of naturally obtained substances. This review presents information about the chemical base of antioxidant activities and beneficial effects on human health of known and new bio-antioxidants. There is abundant literature on the phenolic antioxidants and tocopherols in particular. In this review the following bio-antioxidants are considered: A) Carotenoids, B) Cathecholamines, C) Phospholipids, D) Chalcones, E) Coumarins, F) Phenolic acids, G) Flavonoids, H) Lignans, and I) Tannins.

  12. Antioxidant activities and total phenol content of Inula viscosa extracts selected from three regions of Morocco

    Institute of Scientific and Technical Information of China (English)

    Naima; Chahmi; Jaouad; Anissi; Sanae; Jennan; Abdellah; Farah; Khalid; Sendide; Mohammed; El; Hassouni

    2015-01-01

    Objective:To investigate antioxidant activity,total phenolic and flavonoid content of ethanol(E) and ethyl acetate(A) extracts of Inula viscosa aerial parts selected from three regions of Morocco(Imouzzer.Sefrou and Taounate).Methods:Antioxidants properties were measured by three different test systems of assay namely free radical scavenging activities against 2,2-diphenyI-1-picrylhydrazyl,total antioxidant capacity and iron-reducing capacity.Total phenolic content was measured by FolinCiocalteu reagent.Results:All the extracts showed significant antioxidant activities and contained important levels of phenols.The ethanol extract(0.3 mg/mL) from Sefrou showed the greatest antioxidant capacity in the three systems of assay,which was probably due to its high content of polyphenols(274.39±6.94) mg gallic acid equivalent/g dry extract.Total flavonoid content was found equal for all extracts.Conclusions:Our results of antioxidant assays were justified and partially supported the popular usage of the tested plants.The high antioxidant activity found in the plant from Sefrou and its great biomass in this region suggested that Inula viscosa is a good source of natural antioxidants compounds which might have benefits for health.

  13. In vitro antioxidant activity of Vetiveria Zizanioides root extract.

    Science.gov (United States)

    Subhadradevi, Varadharajan; Asokkumar, Kuppusamy; Umamaheswari, Muthuswamy; Sivashanmugam, Andichettiarthirumalasia; Sankaranand, Rajakannu

    2010-10-01

    Free radicals induce numerous diseases by lipid peroxidation and DNA damage. It has been reported that some of the extracts from plants possess antioxidant properties capable of scavenging free radicals in vivo. Vetiveria zizanioides belonging to the family Gramineae, is a densely tufted grass which is widely used as a traditional plant for aromatherapy, to relieve stress, anxiety, nervous tension and insomnia. In this regard, the roots of V zizanioides was extracted with ethanol and used for the evaluation of various in vitro antioxidant activities such as reducing power ability, superoxide anion radical scavenging activity, deoxyribose degradation assay, total antioxidant capacity, total phenolics and total flavonoid composition. The various antioxidant activities were compared with suitable antioxidants such as butyl hydroxy toluene, ascorbic acid, quercetin, alpha tocopherol, pyrocatechol and curcumin respectively. The generation of free radicals O2, H2O2 OH and N O were effectively scavenged by the ethanolic extract of V zizanioides. In all these methods, the extract showed strong antioxidant activity in a dose dependent manner. The results obtained in the present study clearly indicates that V zizanioides scavenges free radicals, ameliorating damage imposed by oxidative stress in different disease conditions and serve as a potential source of natural antioxidant. The study provides a proof for the ethnomedical claims and reported biological activities. The plant has, therefore, very good therapeutic and antioxidant potential.

  14. Measurement of Antioxidant Activity Towards Superoxide in Natural Waters.

    Directory of Open Access Journals (Sweden)

    D. Whitney King

    2016-11-01

    Full Text Available Antioxidants are a class of molecules that provide a protective function against reactive oxygen species (ROS in biological systems by out competing physiologically important molecules for ROS oxidation. In natural waters, the reactivity of antioxidants gives an estimate of oxidative stress and may determine the reactivity and distribution of reactive oxidants. We present an analytical method to measure antioxidant activity in natural waters through the competition between ascorbic acid, an antioxidant, and MCLA, a chemiluminescent probe for superoxide. A numerical kinetic model of the analytical method has been developed to optimize analytical performance. Measurements of antioxidant concentrations in pure and seawater are possible with detection limits below 0.1 nM. Surface seawater samples collected at solar noon contained over 0.4 nM of antioxidants and exhibited first-order decay with a half-life of 3-7 minutes, consistent with a reactive species capable of scavenging photochemically produced superoxide.

  15. pH dependent antioxidant activity of lettuce (L. sativa) and synergism with added phenolic antioxidants.

    Science.gov (United States)

    Altunkaya, Arzu; Gökmen, Vural; Skibsted, Leif H

    2016-01-01

    Influence of pH on the antioxidant activities of combinations of lettuce extract (LE) with quercetin (QC), green tea extract (GTE) or grape seed extract (GSE) was investigated for both reduction of Fremy's salt in aqueous solution using direct electron spin resonance (ESR) spectroscopy and in L-α-phosphatidylcholine liposome peroxidation assay measured following formation of conjugated dienes. All examined phenolic antioxidants showed increasing radical scavenging effect with increasing pH values by using both methods. QC, GTE and GSE acted synergistically in combination with LE against oxidation of peroxidating liposomes and with QC showing the largest effect. The pH dependent increase of the antioxidant activity of the phenols is due to an increase of their electron-donating ability upon deprotonation and to their stabilization in alkaline solutions leading to polymerization reaction. Such polymerization reactions of polyphenolic antioxidants can form new oxidizable -OH moieties in their polymeric products resulting in a higher radical scavenging activity.

  16. Synthesis and Antioxidant Activity of Hydroxytyrosol Alkyl-Carbonate Derivatives.

    Science.gov (United States)

    Fernandez-Pastor, Ignacio; Fernandez-Hernandez, Antonia; Rivas, Francisco; Martinez, Antonio; Garcia-Granados, Andres; Parra, Andres

    2016-07-22

    Three procedures have been investigated for the isolation of tyrosol (1) and hydroxytyrosol (2) from a phenolic extract obtained from the solid residue of olive milling. These three methods, which facilitated the recovery of these phenols, were chemical or enzymatic acetylation, benzylation, and carbomethoxylation, and subsequent carbonylation or acetonation reactions. Several new lipophilic alkyl-carbonate derivatives of hydroxytyrosol have been synthesized, coupling the primary hydroxy group of this phenol, through a carbonate linker, using alcohols with different chain lengths. The antioxidant properties of these lipophilic derivatives have been evaluated by different methods and compared with free hydroxytyrosol (2) and also with the well-known antioxidants BHT and α-tocopherol. Three methods were used for the determination of this antioxidant activity: FRAP and ABTS assays, to test the antioxidant power in hydrophilic media, and the Rancimat test, to evaluate the antioxidant capacity in a lipophilic matrix. These new alkyl-carbonate derivatives of hydroxytyrosol enhanced the antioxidant activity of this natural phenol, with their antioxidant properties also being higher than those of the commercial antioxidants BHT and α-tocopherol. There was no clear influence of the side-chain length on the antioxidant properties of the alkyl-carbonate derivatives of 2, although the best results were achieved mainly by the compounds with a longer chain on the primary hydroxy group of this natural phenolic substance.

  17. Substituent Effects on the Stability and Antioxidant Activity of Spirodiazaselenuranes

    Directory of Open Access Journals (Sweden)

    Devappa S. Lamani

    2015-07-01

    Full Text Available Spirodiazaselenuranes are structurally interesting compounds and the stability of these compounds depends highly on the nature of the substituents attached to the nitrogen atoms. Aromatic substituents are known to play important roles in stabilizing the Se-N bonds in spiro compounds. In this study, several spirodiazaselenuranes are synthesized by introducing benzylic and aliphatic substituents to understand their effect on the stability of the Se-N bonds and the antioxidant activity. Replacement of phenyl substituent by benzyl/alkyl groups significantly reduces the stability of the spirodiazaselenuranes and slows down the oxidative cyclization process. The selenium centre in the spiro compounds undergoes further oxidation to produce the corresponding selenurane oxides, which are stable at room temperature. Comparison of the glutathione peroxidase (GPx mimetic activity of the compounds showed that the diaryl selenides having heterocyclic rings are significantly more active due to the facile oxidation of the selenium centre. However, the activity is reduced significantly for compounds having aliphatic substituents. In addition to GPx activity, the compounds also inhibit peroxynitrite-mediated nitration and oxidation reaction of protein and small molecules, respectively. The experimental observations suggest that the antioxidant activity is increased considerably upon substitution of the aromatic group with the benzylic/aliphatic substituents on the nitrogen atoms.

  18. HPTLC Analysis, Antioxidant and Antigout Activity of Indian Plants.

    Science.gov (United States)

    Nile, Shivraj Hariram; Park, Se Won

    2014-01-01

    The HPTLC analysis, antioxidant, and antigout activity of Asparagus racemosus, Withania somnifera, Vitex negundo, Plumbago zeylanica, Butea monosperma and Tephrosia purpurea extracts were investigated. The chemical fingerprinting were carried out by high performance thin layer chromatography (HPTLC), antioxidant activity by ABTS, DPPH, FRAP radical scavenging assays, and antiogout activity by cow milk xanthine oxidase. The HPTLC fingerprint qualitatively revealed predominant amount of flavonoids. The TEAC values ranged from 45.80 to 140 µM trolox/100 g dry weight for ABTS, from 85 to 430 µM trolox/ 100 g dw DPPH, and 185 to 560 µM trolox/100 g dw for FRAP respectively. Plants used in this study was found to inhibit the toxicity, as seen from the decreased LPO and increased GSH, SOD and CAT levels. The total phenolic and flavonoid content ranged from 10.21 to 28.17 and 5.80 to 10.1 mg of gallic acid equivalents (GAE)/100 gdw respectively. The plant extracts demonstrated significant xanthine oxidase inhibitory activity at 100 g/mL and revealed an inhibition greater than 50 % and IC50 values below the standard. This effect was almost similar to the activity of allopurinol (Standard drug) against xanthine oxidase (90.2 ± 0.4 %). These plant root extract will be subjected for further extensive studies to isolate and identify their active constituents which are useful for against inflammation and gout.

  19. In vitro antioxidant activities of Solanum surattense leaf extract

    Institute of Scientific and Technical Information of China (English)

    Sridevi Muruhan; Senthil Selvaraj; Pugalendi Kodukkur Viswanathan

    2013-01-01

    Objective: To evaluate the antioxidant activity of alcoholic leaf-extract of Solanum surattense (Solanaceae) (S. surattense). Methods: Leaf extract were tested for in vitro free radical scavenging assays, such as hydroxyl radical and hydrogen peroxide, inhibition of superoxide anion radical and 2, 2-diphenyl-1-picryl hydrazyl radical (DPPH), total antioxidant activity and reducing ability. Further, total phenolic content of S. surattense was analyzed. Results: S. surattense extract effectively scavenged free radicals at all different concentrations and showed its potent antioxidant activity. Further, these effects were in a dose dependent manner. Results were compared to standard antioxidants such as butylated hydroxytoluene, ascorbic acid andα-tocopherol. Conclusions: S. surattense have strong antioxidant potential. Further the study validates the therapeutic benefits of the Indian system of medicine.

  20. In vitro antioxidant activities of Solanum surattense leaf extract

    Institute of Scientific and Technical Information of China (English)

    Sridevi; Muruhan; Senthil; Selvaraj; Pugalendi; Kodukkur; Viswanathan

    2013-01-01

    Objective:To evaluate the antioxidant activity of alcoholic leaf-extract of Solanum surattense(Solanaceae)(S.surattense).Methods:Leaf extract were tested for in vitro free radical scavenging assays,such as hydroxyl radical and hydrogen peroxide,inhibition of superoxide anion radical and 2,2-diphenyl-1-picryl hvdrazyl radical(DPPH),total antioxidant activity and reducing ability.Further,total phenolic content of S.surattense was analyzed.Results:S.surattense extract effectively scavenged free radicals at all different concentrations and showed its potent antioxidant activity.Further,these effects were in a dose dependent manner.Results were compared to standard antioxidants such as butylated hvdroxytoluene,ascorbic acid andα-tocopherol.Conclusions:S.surattense have strong antioxidant potential.Further the study validates the therapeutic benefits of the Indian system of medicine.

  1. Synergistic Antioxidant Activity of Sweet Potato Extracts in Combination with Tea Polyphenols and Pueraria Flavonoid in Vitro

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    Xiaojuan Liu

    2014-02-01

    Full Text Available The antioxidant activity of Sweet Potato Extracts (SPE can be enhanced by the presence of these other active antioxidants such as Tea Polyphones (TP and Pueraria Flavonoid (PF. Since many of these natural antioxidants are consumed together in foods, the potential for synergistic interactions is high in the human diet. The aim of this study was to determine what concentrations and combinations of antioxidants among SPE, TP and PF are capable of producing synergistic antioxidant effects, based on potato-based food products. Solutions of the antioxidant activity of SPE, TP and PF, alone and in different combinations were measured using the stable free radical 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH and Ferric Reducing Anti-oxidant Power (FRAP method. A comparison of the antioxidant activity of the combinations of antioxidants to the arithmetic sum of the antioxidant activity of the individual antioxidants was used to calculate the Synergistic Effects (SEs between the antioxidants. The results showed that all concentrations of TP and PF combination with SPE (1 and 1.5% could produce significant SEs (p<0.05 of DPPH and FRAP for the two or three component mixtures. With the concentration of 1% SPE, 1×10-5 g/mL TP and 5×10-5 g/mL PF in the three-component mixture, the highest SE of DPPH and FRAP was both detected. The results suggested that the antioxidant property of this combination was substantially superior to the sum of the individual antioxidant effects and these interactions can enhance the antioxidant effectiveness of SPE. The results could guide in the formulation and development of functional food products that have high antioxidant potential.

  2. CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF APIS MELLIFERA BEE POLLEN FROM NORTHWEST ALGERIA

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    A. Rebiai

    2012-12-01

    Full Text Available The aim of this study was to assess the nutritional composition and antioxidant activity of bee pollen produced in the North western region of Algeria. The content of total phenolic compounds, flavonoids, and antioxidant activity using the molybdate ion reduction method were determined. The mean contents of phenolic compounds and total flavonoids were 30.46 ± 8.22 mg of GAE.g–1 pollen and 8.92 ± 5.5 mg of RE.g–1 pollen, respectively. High antioxidant activities were found for the molybdate ion reduction method, with values that ranged from 71.95 to 101.5 μg of GAE.g–1 pollen. The bee pollen of Boufarik showed high antioxidant activity probably due to the high content of phenolic compounds present in pollen.

  3. The antioxidant activity of selected Romanian honeys

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    Irina DOBRE

    2010-12-01

    Full Text Available Four types of Apis mellifera honey collected in the eastern region of Romania were screened for their total phenolic content by the Folin-Ciocalteau method, for anti radical power as assessed by DPPHradical scavenging assay, for the FRAP assay-ferric reducing antioxidant power. The antioxidant effect over a substrate sensitive to lipid per oxidation and also the presence of some pigments with antioxidant action in honey-ABS 450 were evaluated. All assays revealed the following order of the obtained values: Lime honey>poly-flower honey>Sea buckthorn honey>Acacia honey.

  4. Catalytic synthesis and antioxidant activity of sulfated polysaccharide from Momordica charantia L.

    Science.gov (United States)

    Liu, Xin; Chen, Tong; Hu, Yan; Li, Kexin; Yan, Liushui

    2014-03-01

    Sulfated derivatives of polysaccharide from Momordica charantia L. (MCPS) with different degree of sulfation (DS) were synthesized by chlorosulfonic acid method with ionic liquids as solvent. Fourier transform infrared spectra and 13C nuclear magnetic resonance spectra indicated that C-6 substitution was predominant in MCPS compared with the C-2 position. Compared with the native polysaccharide from Momordica charantia L. (MCP), MCPS exhibited more excellent antioxidant activities in vitro, which indicated that sulfated modification could enhance antioxidant activities of MCP. Furthermore, high DS and moderate molecular weight could improve the antioxidant activities of polysaccharide. Copyright © 2013 Wiley Periodicals, Inc.

  5. Evaluation of antioxidant activity of essential oil from Ajwain (Trachyspermum ammi seeds

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    Sreemoyee Chatterjee

    2013-01-01

    Full Text Available Background: Free radicals provoke chain reactions within the cell and cause damage to the cell. Oxidation of bio molecules such as carbohydrate, protein, lipid and nucleic acid leads to the production of free radicals which induce the onset of disease. Antioxidants scavenge these free radicals, thereby protecting the cell from damage. Phenolic compounds found in varieties of seeds and fruits are thought to have antioxidant activities. Aim: The present study was designed to examine the in vitro antioxidant activity of Trachyspermum ammi (Ajwain essential oil. Materials and Methods: The antioxidant activity of the oil was assessed by using 2, 2-diphenyl-1-picryl-hydrazyl (DPPH, H 2 O 2 radical scavenging activity and ferric reducing antioxidant power (FRAP. The values were compared with those obtained with standard antioxidant, ascorbic acid. Results: It was determined that the essential oil possessed a high degree of FRAP followed by a good DPPH radical scavenging activity and a moderate H 2 O 2 radical scavenging activity. Conclusion: This study concludes that the essential oil of Ajwain could contribute as a highly significant bio resource of antioxidants to be used in our day-to-day life in food and pharmaceutical industry.

  6. Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements.

    Science.gov (United States)

    Wolfe, Kelly L; Liu, Rui Hai

    2007-10-31

    A cellular antioxidant activity (CAA) assay for quantifying the antioxidant activity of phytochemicals, food extracts, and dietary supplements has been developed. Dichlorofluorescin is a probe that is trapped within cells and is easily oxidized to fluorescent dichlorofluorescein (DCF). The method measures the ability of compounds to prevent the formation of DCF by 2,2'-azobis(2-amidinopropane) dihydrochloride (ABAP)-generated peroxyl radicals in human hepatocarcinoma HepG2 cells. The decrease in cellular fluorescence when compared to the control cells indicates the antioxidant capacity of the compounds. The antioxidant activities of selected phytochemicals and fruit extracts were evaluated using the CAA assay, and the results were expressed in micromoles of quercetin equivalents per 100 micromol of phytochemical or micromoles of quercetin equivalents per 100 g of fresh fruit. Quercetin had the highest CAA value, followed by kaempferol, epigallocatechin gallate (EGCG), myricetin, and luteolin among the pure compounds tested. Among the selected fruits tested, blueberry had the highest CAA value, followed by cranberry > apple = red grape > green grape. The CAA assay is a more biologically relevant method than the popular chemistry antioxidant activity assays because it accounts for some aspects of uptake, metabolism, and location of antioxidant compounds within cells.

  7. Antibacterial activities, antioxidant contents and antioxidant properties of three traditional Chinese medicinal extracts

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    Wenbin Li

    2015-03-01

    Full Text Available The present study was carried out to identify antibacterial and antioxidant characteristics of traditional aqueous extracts derived from three traditional Chinese medicinal plants (Scutellaria baicalensis, Coptis chinensis and Sonchus oleraceus. It was indicated that the S. oleraceus showed the highest antibacterial efficacy, especially against Staphylococcus aureus. The minimum inhibitory concentration (MIC of the S. oleraceus was 5.0 mg/mL what was in correlation with the high total phenolic and flavonoid contents and CUPRAC value, and MIC of both S. baicalensis and C. chinensis was 7.5 mg/mL. The rational pH of the working S. oleraceus was acidic, while the other two preferred to neutral or alkaline environment. The reasonable preservation temperature of S. baicalensis should not beyond 60°C, while the other two below 90°C. Meanwhile, S. baicalensis had significant antioxidant activity with the highest CUPRAC and ·OH scavenging activity. These results had provided useful information on further drug discovery.

  8. Effect of freeze-drying on the antioxidant compounds and antioxidant activity of selected tropical fruits.

    Science.gov (United States)

    Shofian, Norshahida Mohamad; Hamid, Azizah Abdul; Osman, Azizah; Saari, Nazamid; Anwar, Farooq; Dek, Mohd Sabri Pak; Hairuddin, Muhammad Redzuan

    2011-01-01

    The effects of freeze-drying on antioxidant compounds and antioxidant activity of five tropical fruits, namely starfruit (Averrhoa carambola L.), mango (Mangifera indica L.), papaya (Carica papaya L.), muskmelon (Cucumis melo L.), and watermelon Citruluss lanatus (Thunb.) were investigated. Significant (p 0.05) change, however, observed in the ascorbic acid content of the fresh and freeze-dried fruits. Similarly, freeze-drying did not exert any considerable effect on β-carotene concentration of fruits, except for mango and watermelon, where significantly (p inhibition measurement, a significant (p < 0.05) but random variation was recorded between the fresh and freeze-dried fruits. Overall, in comparison to β-carotene and ascorbic acid, a good correlation was established between the result of TPC and antioxidant assays, indicating that phenolics might have been the dominant compounds contributing towards the antioxidant activity of the fruits tested.

  9. Chocolate consumption, fecal water antioxidant activity, and hydroxyl radical production.

    Science.gov (United States)

    Record, Ian R; McInerney, Jennifer K; Noakes, Manny; Bird, Anthony R

    2003-01-01

    As part of a larger study into the effects of polyphenols derived from chocolate on bowel health we have compared the effects of consumption of chocolate containing either 200 mg of flavanols and related procyanidins or a similar chocolate containing less than 10 mg of polyphenols on fecal free radical production and antioxidant activity in 18 volunteers. In a double-blind crossover trail volunteers consumed chocolate for two 4-wk periods separated by a 4-wk washout period. During the time the volunteers consumed the chocolate they also consumed a low-polyphenol diet. Free radical production in the fecal water was lowered from 122 +/- 10 micromol/l/h to 94 +/- 9 micromol/l/h (P = 0.009) when the high procyanidin chocolate diet was consumed and from 117 +/- 14 micromol/l/h to 86 +/- 12 micromol/l/h when the low procyanidin chocolate was consumed (P = 0.014). Fecal water antioxidant capacity measured by either the Trolox equivalent antioxidant capacity or ferric reducing ability of plasma procedure was not significantly affected. Consumption of either chocolate reduced the production of free radicals in fecal water. This suggests that some component of the chocolate other than the flavanols and related procyanidins may have been effective.

  10. In vitro antioxidant activity study of novel chromone derivatives.

    Science.gov (United States)

    Phosrithong, Narumol; Samee, Weerasak; Nunthanavanit, Patcharawee; Ungwitayatorn, Jiraporn

    2012-06-01

    Forty-eight chromone derivatives were evaluated for their antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay, ferrous ions (Fe(2+) ) chelating activity test, total antioxidant activity test (Ferric thiocyanate and Thiobarbituric acid methods), and total reductive capability (potassium ferricyanide reduction). 7,8-Dihydroxy-2-(3'-trifluoromethylphenyl)-3-(3″-trifluoromethylbenzoyl) chromone 32 showed stronger radical scavenging and metal chelating activities than butylated hydroxytoluene, vitamin E, and trolox. Chromone derivatives that exhibited good radical scavenging and metal chelating also displayed strong total antioxidant and reductive power activities. The results obtained from this study indicated that the synthesized chromone derivatives have remarkable antioxidant activity. © 2012 John Wiley & Sons A/S.

  11. Screening of antioxidant activity in microalgae

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    Mariana F.G. Assunção

    2014-06-01

    Both sets of results indicate an interesting antioxidant potential in microalgae belonging to the groups Eustigmatophyceae and Chlorophyceae. Tested species of these groups showed ABTS•+ values comparable to grape and raspberry ethanolic extracts, confirmed also by the DPPH• method.

  12. Determination of tea components with antioxidant activity.

    Science.gov (United States)

    Cabrera, Carmen; Giménez, Rafael; López, M Carmen

    2003-07-16

    Levels of essential elements with antioxidant activity, as well as catechins, gallic acid, and caffeine levels, in a total of 45 samples of different teas commercialized in Spain have been evaluated. Chromium, manganese, selenium, and zinc were determined in the samples mineralized with HNO(3) and V(2)O(5), using ETAAS as the analytical technique. The reliability of the procedure was checked by analysis of a certified reference material. Large variations in the trace element composition of teas were observed. The levels ranged from 50.6 to 371.4 ng/g for Cr, from 76.1 to 987.6 microg/g for Mn, from 48.5 to 114.6 ng/g for Se, and from 56.3 to 78.6 ng/g for Zn. The four major catechins [(-)-epigallocatechin gallate (EGCG), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epicatechin (EC)], gallic acid (GA), and caffeine were simultaneously determined by a simple and fast HPLC method using a photodiode array detector. In all analyzed samples, EGCG ranged from 1.4 to 103.5 mg/g, EGC from 3.9 to 45.3 mg/g, ECG from 0.2 to 45.6 mg/g, and EC ranged from 0.6 to 21.2 mg/g. These results indicated that green tea has a higher content of catechins than both oolong and fermented teas (red and black teas); the fermentation process during tea manufacturing reduces the levels of catechins significantly. Gallic acid content ranged from 0.039 to 6.7 mg/g; the fermentation process also elevated remarkably gallic acid levels in black teas (mean level of 3.9 +/- 1.5 mg/g). The amount of caffeine in the analyzed samples ranged from 7.5 to 86.6 mg/g, and the lower values were detected in green and oolong teas. This study will be useful for the appraisal of trace elements and antioxidant components in various teas, and it will also be of interest for people who like drinking this beverage.

  13. ANTIOXIDANT ACTIVITY OF WINTER SLAVONIAN GARLIC (Allium sativum L.

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    Nada Parađiković

    2012-12-01

    Full Text Available Numerous studies conducted on garlic (Allium sativum L., have proved the presence of antioxidants, phenolic compounds, sulfur compounds and several vitamins. The aim of this study was to investigate the differences in the content of total phenols, ascorbic acid; total antioxidant activities of four winter Slavonian garlic cultivars, as well as establishment of correlations between these parameters. The total antioxidant activity and the content of ascorbic acid and total phenols in four cultivars of winter Slavonian garlic were spectrophotometrically determined. Genetic diversity of winter Slavonian garlic cultivars significantly affected the content of ascorbic acid and the total antioxidant activity. The total antioxidant activity significantly positively correlated with the content of ascorbic acid (r2 = 0.64, p = 0.006, while the content of total phenols and total antioxidant activity were not significantly related. Cultivar PFO 1 had the highest antioxidant activity (8.006 μmol Trolox /g FW and the highest ascorbic acid content (8.57 mg /100 g FW, thus, the best quality of the four tested garlic cultivars.

  14. IN VITRO ANTIOXIDANT ACTIVITY OF CURCUMA AMADA ROXB.

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    Sanjay Jain, Rakesh Barik* and Arti Jain

    2013-02-01

    Full Text Available A study was undertaken to evaluate the antioxidant potential of rhizomes of Curcuma amada (Zingiberaceae. Aqueous and ethanolic extracts were subjected to in vitro antioxidant activity screening models such as DPPH, ABTS, nitric oxide and inhibition of lipid peroxidation activity. Ascorbic acid was used as the standard. In all the models studied, both the extracts showed nearly equal activities thereby justifying the traditional claims of the plant.

  15. Antioxidant activity of Fish Protein Hydrolysates from Sardinella longiceps

    OpenAIRE

    JEEVITHA K; MOHANA PRIYA K; SAMANTA S. KHORA

    2014-01-01

    Sardinella longiceps were hydrolysed with the different concentration of proteolytic enzymes trypsin to obtain peptides with antioxidant activity. The degree of hydrolysis and yield of hydrolysates were found increasing with increasing the concentration of enzyme. The antioxidant activities of hydrolysates were investigated through various assays. The hydrolysate exhibited the higher reducing power capability. The hydrolysates has shown a higher scavenging activity against DPPH, Superoxide, h...

  16. Study of Antioxidant Activity of Juglans regia leaves

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    FARHAD HATAMJAFARI

    2013-06-01

    Full Text Available This study was designed to investigate the antioxidant activity for Juglans regia leaves. The Methanol extract from Iranian Juglans regia leaves that grow in Karaj and Tonekabon regions was examined. In addition, total amount of DPPH (1,1-diphenyl-2-picryl hydrazyl radical scavenging activities and reductive power of crude extracted its different fractions were determined. The antioxidant activity data by DPPH has been compared in both regions.

  17. Radical scavenging, antioxidant and antimicrobial activities of halophytic species

    OpenAIRE

    Meot-Duros, Laetitia; Le Floch, Gaëtan; Magné, Christian

    2008-01-01

    International audience; For the first time, both antioxidant and antimicrobial activities are simultaneously reported in halophytic plants, particularly on polar fractions. Chloroformic and methanolic extracts of the halophytes Eryngium maritimum L., Crithmum maritimum L. and Cakile maritima Scop. were tested for their antimicrobial activities against 12 bacterial and yeast strains. In addition, radical scavenging and antioxidant activities were assessed, as well as total phenol contents. Onl...

  18. Role of degradation products of chlorogenic acid in the antioxidant activity of roasted coffee.

    Science.gov (United States)

    Kamiyama, Masumi; Moon, Joon-Kwan; Jang, Hae Won; Shibamoto, Takayuki

    2015-02-25

    Antioxidant activities of brewed coffees prepared from six commercial brands ranged from 63.13 ± 1.01 to 96.80 ± 1.68% at the highest levels tested. Generally, the degree of antioxidant activity of the brewed coffee was inversely proportional to the total chlorogenic acid concentration. A sample obtained from the major chlorogenic acid, 5-caffeoylquinic acid (5-CQA), heated at 250 °C exhibited potent antioxidant activity (79.12 ± 2.49%) at the level of 10 μg/mL, whereas unheated 5-CQA showed only moderate antioxidant activity (44.41 ± 0.27%) at the level of 100 μg/mL. Heat produced relatively high levels of pyrocatechol (2,809.3 μg/g) and 2-methoxy-4-vinylphenol (46.4 μg/g) from 5-CQA, and their antioxidant activity levels were 76.57 ± 3.00 and 98.63 ± 0.01%, respectively. The results of the present study suggest that roasting degrades chlorogenic acids to form potent antioxidants and thus plays an important role in the preparation of high-antioxidant low-acid coffee.

  19. Antioxidant Activities of Chemical Constituents Isolated from Echinops orientalis Trauv.

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    Ramazan Erenler

    2014-01-01

    Full Text Available The genus Echinops belonging to the Asteraceae family comprises 130 species. The dried leaves and seeds of Echinops orientalis Trauv. were extracted separately with methanol. Apigenin-7-O-(6"-trans-p-coumaroyl- b -D-glucopyranoside 1, and Apigenin-7-O- b -D glucoside 2 were isolated from leaves and 1-methoxycarbonylindole 3 and beta-sitositerol 4 were isolated from seeds. The compounds were isolated by chromatographic techniques, based on column chromatography, preparative TLC and identified by spectroscopic methods including 1D-, 2D-NMR, UV, IR, HPLC-QTOF/MS. Isolated compounds and extracts were applied to antioxidant activity tests. While s eeds and leaves extracts have high DPPH and moderate ABTS radical scavenging activities, the isolated flavones exhibited high cation radical scavenging activities.

  20. Antioxidant activity of different fractions of Spirulina platensis protean extract.

    Science.gov (United States)

    Piñero Estrada, J E; Bermejo Bescós, P; Villar del Fresno, A M

    2001-01-01

    Spirulina platensis, planktonic blue-green algae, is gaining increasing attention because of its nutritional and medicinal properties. This microalgae contains phycobiliproteins (phycocyanin and allophycocyanin). Previous reports from our laboratory have shown that a protean extract of S. platensis is a potent free-radical scavenger (hydroxyl and peroxyl radicals) and inhibits microsomal lipid peroxidation. The aim of this study was to purify and characterize phycocyanin of S. platensis. Besides, we tried to demonstrate that one of the main components responsible for this antioxidant activity is a biliprotein phycocyanin. For this purpose, we studied the antioxidant activity of different fractions obtained during the phycocyanin purification process, through the scavenger activity of hydroxyl radical. We also observed that an increase in phycocyanin content was related to an increase in the antioxidant activity in different fractions, and therefore phycobiliprotein phycocyanin is the component mainly responsible for the antioxidant activity.

  1. Antioxidant and antimicrobial activities of Saraca thaipingensis Cantley ex Prain

    Institute of Scientific and Technical Information of China (English)

    Supaluk Prachayasittikul; Apilak Worachartcheewan; Sakda Yainoy; Natthakaln Lomchoey; Paveena Kittiphatcharin; Somsak Ruchirawat; Virapong Prachayasittikul

    2012-01-01

    Objective: To investigate antioxidant and antimicrobial activities of Saraca thaipingensis Cantley ex Prain; and isolation of its flower extracts. Methods: The plant species (flowers, leaves, and twigs) were extracted by hexane, dichloromethane, ethyl acetate and methanol; and tested for antioxidant activity (DPPH assay) and antimicrobial activity (agar dilution method) against twenty-seven strains of microorganisms; gram positive and gram negative bacteria, and diploid fungus. Bioactive constituents were isolated by column chromatography. Results: The plant extracts has been firstly reported to display strong antioxidant activity and antimicrobial activity selective against gram positive bacteria (Corynebacterium diphtheriae NCTC 10356 and Streptococcus pyogenes) with MIC of 256 μg/mL. Stigmasterol and a mixture of triterpenoids and phenolic compounds were isolated from the flower extracts. Conclusions: The study revealed that the S. thaipingensis is a new source of natural antioxidants and antimicrobials with potential for medicinal uses.

  2. Synthesis and Antioxidant Activity of Alkyl Nitroderivatives of Hydroxytyrosol

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    Elena Gallardo

    2016-05-01

    Full Text Available A series of alkyl nitrohydroxytyrosyl ether derivatives has been synthesized from free hydroxytyrosol (HT, the natural olive oil phenol, in order to increase the assortment of compounds with potential neuroprotective activity in Parkinson’s disease. In this work, the antioxidant activity of these novel compounds has been evaluated using Ferric Reducing Antioxidant Power (FRAP, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt (ABTS, and Oxygen Radical Scavenging Capacity (ORAC assays compared to that of nitrohydroxytyrosol (NO2HT and free HT. New compounds showed variable antioxidant activity depending on the alkyl side chain length; compounds with short chains (2–4 carbon atoms maintained or even improved the antioxidant activity compared to NO2HT and/or HT, whereas those with longer side chains (6–8 carbon atoms showed lower activity than NO2HT but higher than HT.

  3. Brazilian red propolis: unreported substances, antioxidant and antimicrobial activities.

    Science.gov (United States)

    Righi, Adne A; Alves, Thiago R; Negri, Giuseppina; Marques, Lucas M; Breyer, Henrique; Salatino, Antonio

    2011-10-01

    Chloroform, ethyl acetate and methanol extracts of a sample of red propolis from the state of Alagoas (northeast Brazil) were analyzed by gas chromatography-mass spectrometry and high-performance liquid chromatography-diode array detection-electrospray ionization-mass spectrometry. Antimicrobial and antioxidant activities were also obtained. The propolis sample contained low content of narigenin-8-C-hexoside, this being the first report of a C-glycoside in propolis. The main constituent found was characterized as 3,4,2',3'-tetrahydroxychalcone. Other important constituents were the chalcone isoliquiritigenin, the isoflavans (3S)-vestitol, (3S)-7-O-methylvestitol, the pterocarpan medicarpin, the phenylpropenes trans-anethol, methyl eugenol, elimicin, methoxyeugenol and cis-asarone, and the triterpenic alcohols lupeol and α- and β- amyrins. The methanol extract exhibited high antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl and β-carotene/linoleic acid assay methods, and antimicrobial activity toward Gram-positive and Gram-negative bacteria. Structures are suggested for new substances never before seen in any kind of propolis. This is the first report of 3,4,2',3'-tetrahydroxychalcone and a flavone C-glycoside in a propolis sample. Copyright © 2011 Society of Chemical Industry.

  4. Antioxidant and antimicrobial activities of cinnamic acid derivatives.

    Science.gov (United States)

    Sova, M

    2012-07-01

    Cinnamic acid is an organic acid occurring naturally in plants that has low toxicity and a broad spectrum of biological activities. In the search for novel pharmacologically active compounds, cinnamic acid derivatives are important and promising compounds with high potential for development into drugs. Many cinnamic acid derivatives, especially those with the phenolic hydroxyl group, are well-known antioxidants and are supposed to have several health benefits due to their strong free radical scavenging properties. It is also well known that cinnamic acid has antimicrobial activity. Cinnamic acid derivatives, both isolated from plant material and synthesized, have been reported to have antibacterial, antiviral and antifungal properties. Acids, esters, amides, hydrazides and related derivatives of cinnamic acid with such activities are here reviewed.

  5. Angiotensin-I-Converting Enzyme Inhibitory and Antioxidant Activities of Protein Hydrolysate from Muscle of Barbel (Barbus callensis)

    OpenAIRE

    Assaad Sila; Anissa Haddar; Oscar Martinez-Alvarez; Ali Bougatef

    2013-01-01

    The present study investigated angiotensin-I-converting enzyme (ACE) inhibitory and antioxidant activities of barbel muscle protein hydrolysate prepared with Alcalase. The barbel muscle protein hydrolysate displayed a high ACE inhibitory activity (C I 50 = 0.92 mg/mL). The antioxidant activities of protein hydrolysate at different concentrations were evaluated using various in vitro antioxidant assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical method and reducing power assay. The...

  6. Chemical Composition and Antioxidant Activity of Euterpe oleracea Roots and Leaflets

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    Christel Brunschwig

    2016-12-01

    Full Text Available Euterpe oleracea (açaí is a palm tree well known for the high antioxidant activity of its berries used as dietary supplements. Little is known about the biological activity and the composition of its vegetative organs. The objective of this study was to investigate the antioxidant activity of root and leaflet extracts of Euterpe oleracea (E. oleracea and characterize their phytochemicals. E. oleracea roots and leaflets extracts were screened in different chemical antioxidant assays (DPPH—2,2-diphenyl-1-picrylhydrazyl, FRAP—ferric feducing antioxidant power, and ORAC—oxygen radical absorbance capacity, in a DNA nicking assay and in a cellular antioxidant activity assay. Their polyphenolic profiles were determined by UV and LC-MS/MS. E. oleracea leaflets had higher antioxidant activity than E. oleracea berries, and leaflets of Oenocarpus bacaba and Oenocarpus bataua, as well as similar antioxidant activity to green tea. E. oleracea leaflet extracts were more complex than root extracts, with fourteen compounds, including caffeoylquinic acids and C-glycosyl derivatives of apigenin and luteolin. In the roots, six caffeoylquinic and caffeoylshikimic acids were identified. Qualitative compositions of E. oleracea, Oenocarpus bacaba and Oenocarpus bataua leaflets were quite similar, whereas the quantitative compositions were quite different. These results provide new prospects for the valorization of roots and leaflets of E. oleracea in the pharmaceutical, food or cosmetic industry, as they are currently by-products of the açaí industry.

  7. Chemical Composition and Antioxidant Activity of Euterpe oleracea Roots and Leaflets

    Science.gov (United States)

    Brunschwig, Christel; Leba, Louis-Jérôme; Saout, Mona; Martial, Karine; Bereau, Didier; Robinson, Jean-Charles

    2016-01-01

    Euterpe oleracea (açaí) is a palm tree well known for the high antioxidant activity of its berries used as dietary supplements. Little is known about the biological activity and the composition of its vegetative organs. The objective of this study was to investigate the antioxidant activity of root and leaflet extracts of Euterpe oleracea (E. oleracea) and characterize their phytochemicals. E. oleracea roots and leaflets extracts were screened in different chemical antioxidant assays (DPPH—2,2-diphenyl-1-picrylhydrazyl, FRAP—ferric feducing antioxidant power, and ORAC—oxygen radical absorbance capacity), in a DNA nicking assay and in a cellular antioxidant activity assay. Their polyphenolic profiles were determined by UV and LC-MS/MS. E. oleracea leaflets had higher antioxidant activity than E. oleracea berries, and leaflets of Oenocarpus bacaba and Oenocarpus bataua, as well as similar antioxidant activity to green tea. E. oleracea leaflet extracts were more complex than root extracts, with fourteen compounds, including caffeoylquinic acids and C-glycosyl derivatives of apigenin and luteolin. In the roots, six caffeoylquinic and caffeoylshikimic acids were identified. Qualitative compositions of E. oleracea, Oenocarpus bacaba and Oenocarpus bataua leaflets were quite similar, whereas the quantitative compositions were quite different. These results provide new prospects for the valorization of roots and leaflets of E. oleracea in the pharmaceutical, food or cosmetic industry, as they are currently by-products of the açaí industry. PMID:28036089

  8. Antioxidant, Antimicrobial and Antiproliferative Activities of Five Lichen Species

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    Snežana Marković

    2011-08-01

    Full Text Available The antioxidative, antimicrobial and antiproliferative potentials of the methanol extracts of the lichen species Parmelia sulcata, Flavoparmelia caperata, Evernia prunastri, Hypogymnia physodes and Cladonia foliacea were evaluated. The total phenolic content of the tested extracts varied from 78.12 to 141.59 mg of gallic acid equivalent (GA/g of extract and the total flavonoid content from 20.14 to 44.43 mg of rutin equivalent (Ru/g of extract. The antioxidant capacities of the lichen extracts were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH radicals scavenging. Hypogymnia physodes with the highest phenolic content showed the strongest DPPH radical scavenging effect. Further, the antimicrobial potential of the lichen extracts was determined by a microdilution method on 29 microorganisms, including 15 strains of bacteria, 10 species of filamentous fungi and 4 yeast species. A high antimicrobial activity of all the tested extracts was observed with more potent inhibitory effects on the growth of Gram (+ bacteria. The highest antimicrobial activity among lichens was demonstrated by Hypogymnia physodes and Cladonia foliacea. Finally, the antiproliferative activity of the lichen extracts was explored on the colon cancer adenocarcinoma cell line HCT-116 by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide viability assay and acridine orange/ethidium bromide staining. The methanol extracts of Hypogymnia physodes and Cladonia foliacea showed a better cytotoxic activity than the other extracts. All lichen species showed the ability to induce apoptosis of HCT-116 cells.

  9. Biological and Nonbiological Antioxidant Activity of Some Essential Oils.

    Science.gov (United States)

    Pérez-Rosés, Renato; Risco, Ester; Vila, Roser; Peñalver, Pedro; Cañigueral, Salvador

    2016-06-15

    Fifteen essential oils, four essential oil fractions, and three pure compounds (thymol, carvacrol, and eugenol), characterized by gas chromatography and gas chromatography-mass spectrometry, were investigated for biological and nonbiological antioxidant activity. Clove oil and eugenol showed strong DPPH (2,2-diphenyl-1-picrylhydrazyl) free-radical scavenging activity (IC50 = 13.2 μg/mL and 11.7 μg/mL, respectively) and powerfully inhibited reactive oxygen species (ROS) production in human neutrophils stimulated by PMA (phorbol 12-myristate 13-acetate) (IC50 = 7.5 μg/mL and 1.6 μg/mL) or H2O2 (IC50 = 22.6 μg/mL and 27.1 μg/mL). Nutmeg, ginger, and palmarosa oils were also highly active on this test. Essential oils from clove and ginger, as well as eugenol, carvacrol, and bornyl acetate inhibited NO (nitric oxide) production (IC50 oils of clove, red thyme, and Spanish oregano, together with eugenol, thymol, and carvacrol showed the highest myeloperoxidase inhibitory activity. Isomers carvacrol and thymol displayed a disparate behavior in some tests. All in all, clove oil and eugenol offered the best antioxidant profile.

  10. Antioxidant, antimicrobial and antiproliferative activities of five lichen species.

    Science.gov (United States)

    Mitrović, Tatjana; Stamenković, Slaviša; Cvetković, Vladimir; Tošić, Svetlana; Stanković, Milan; Radojević, Ivana; Stefanović, Olgica; Comić, Ljiljana; Dačić, Dragana; Curčić, Milena; Marković, Snežana

    2011-01-01

    The antioxidative, antimicrobial and antiproliferative potentials of the methanol extracts of the lichen species Parmelia sulcata, Flavoparmelia caperata, Evernia prunastri, Hypogymnia physodes and Cladonia foliacea were evaluated. The total phenolic content of the tested extracts varied from 78.12 to 141.59 mg of gallic acid equivalent (GA)/g of extract and the total flavonoid content from 20.14 to 44.43 mg of rutin equivalent (Ru)/g of extract. The antioxidant capacities of the lichen extracts were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals scavenging. Hypogymnia physodes with the highest phenolic content showed the strongest DPPH radical scavenging effect. Further, the antimicrobial potential of the lichen extracts was determined by a microdilution method on 29 microorganisms, including 15 strains of bacteria, 10 species of filamentous fungi and 4 yeast species. A high antimicrobial activity of all the tested extracts was observed with more potent inhibitory effects on the growth of Gram (+) bacteria. The highest antimicrobial activity among lichens was demonstrated by Hypogymnia physodes and Cladonia foliacea. Finally, the antiproliferative activity of the lichen extracts was explored on the colon cancer adenocarcinoma cell line HCT-116 by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) viability assay and acridine orange/ethidium bromide staining. The methanol extracts of Hypogymnia physodes and Cladonia foliacea showed a better cytotoxic activity than the other extracts. All lichen species showed the ability to induce apoptosis of HCT-116 cells.

  11. Antioxidant activity in selected Slovenian organic and conventional crops

    Directory of Open Access Journals (Sweden)

    Manca KNAP

    2015-12-01

    Full Text Available The demand for organically produced food is increasing. There is widespread belief that organic food is substantially healthier and safer than conventional food. According to literature organic food is free of phytopharmaceutical residues, contain less nitrates and more antioxidants. The aim of the present study was to verify if there are any differences in the antioxidant activity between selected Slovenian organic and conventional crops. Method of DPPH (2,2-diphenyl-1-picryhydrazyl was used to determine the antioxidant activity of 16 samples from organic and conventional farms. The same varieties of crops were analysed. DPPH method was employed to measure the antioxidant activity of polar antioxidants (AAp and antioxidant activity of fraction in ethyl acetate soluble antioxidants (EA AA. Descriptive statistics and variance analysis were used to describe differences between farming systems. Estimated differences between interactions for the same crop and different farming practice were mostly not statistically significant except for the AAp for basil and beetroot. Higher statistically significant values were estimated for conventional crops. For the EA AA in broccoli, cucumber, rocket and cherry statistically significant higher values were estimated for organic production.

  12. A Caco-2 cell-based quantitative antioxidant activity assay for antioxidants.

    Science.gov (United States)

    Wan, Hongxia; Liu, Dong; Yu, Xiangying; Sun, Haiyan; Li, Yan

    2015-05-15

    A Caco-2 cell-based antioxidant activity (CAA) assay for quantitative evaluation of antioxidants was developed by optimizing seeding density and culture time of Caco-2 cells, incubation time and concentration of fluorescent probe (2',7'-dichlorofluorescin diacetate, DCFH-DA), incubation way and incubation time of antioxidants (pure phytochemicals) and DCFH-DA with cells, and detection time of fluorescence. Results showed that the CAA assay was of good reproducibility and could be used to evaluate the antioxidant activity of antioxidants at the following conditions: seeding density of 5 × 10(4)/well, cell culture time of 24h, co-incubation of 60 μM DCFH-DA and pure phytochemicals with Caco-2 cells for 20 min and fluorescence recorded for 90 min. Additionally, a significant correlation was observed between CAA values and rat plasma ORAC values following the intake of antioxidants for selected pure phytochemicals (R(2) = 0.815, p < 0.01), demonstrating the good biological relevance of CAA assay. Copyright © 2014 Elsevier Ltd. All rights reserved.

  13. Antioxidant Activity of the Successive Extracts of Aesculus indica Leaves

    Directory of Open Access Journals (Sweden)

    Guno Sindhu Chakraborthy

    2009-07-01

    Full Text Available Plants are the best source of active secondary metabolites which are beneficial to mankind. Many plant origin drugs have been reported with biological properties like Analgesic, Anti-inflammatory, Antioxidant, hypoglycemic agents and many more. The successive extracts of Aesculus indica leaves were screened for in vitro antioxidant properties using the standard procedures. The successive extracts such as petroleum ether, ethyl acetate, methanol and water and 50% crude methanol extracts exhibited IC 50 values of respectively in DPPH and respectively in nitric oxide radical inhibition assays. The values are comparable with the standards such as ascorbic acid and quercetin. The Aesculus indica leaves are showing significant antioxidant activity.

  14. In vitro Cytotoxic and Antioxidant Activity of Leaf Extracts of ...

    African Journals Online (AJOL)

    Results: The methanol extract showed the highest antioxidant activity (DPPH, half maximal inhibitory concentration ..... content in Burkina Fasan honey, as well as their radical scavenging ... Studies of the in vitro anticancer, antimicrobial and.

  15. Antioxidant activity and total phenolics of some mangroves in ...

    African Journals Online (AJOL)

    SERVER

    2008-03-18

    Mar 18, 2008 ... The antioxidant activity of 23 extracts from different plant parts (leaves, stem bark and root) of 6 mangroves and 4 ... situations of oxidative stress reactive oxygen species ... These ROS play an important role in the etiology and.

  16. Antioxidant and free radical scavenging activities of edible weeds ...

    African Journals Online (AJOL)

    ... hydrogen peroxide, and hydroxyl, nitric oxide radicals, play an important role in ... Active (or reactive) oxygen species and free radical-mediated reactions are ... of medicinal plants as antioxidants in reducing such free radical induced tissue ...

  17. Cytotoxicity, phytochemical analysis and antioxidant activity of crude ...

    African Journals Online (AJOL)

    Cytotoxicity, phytochemical analysis and antioxidant activity of crude extracts from ... of both species was studied using the brine shrimp lethality tests (BST) for the first time. ... Both aqueous and methanol extracts of the two medicinal plants ...

  18. Alpha-Glucosidase Inhibitory and Antioxidant Activity of Solvent ...

    African Journals Online (AJOL)

    Methods: Extracts were prepared using hexane, chloroform, ethyl acetate, acetone (AE), methanol, ... screening has revealed moderate antiglucosidase and antioxidant activities in the ... glucosidase inhibitor in vivo and in vitro has been.

  19. In vitro antioxidant activity of Vetiveria zizanioides root extract

    African Journals Online (AJOL)

    In all these methods, the extract showed strong antioxidant activity in a dose dependent manner. ... Preliminary phytochemical screening of the powdered leaves was performed for the presence of ..... Using various in vitro and ex vivo models.

  20. ANTIOXIDANT AND ANTITUMOR ACTIVITY OF INDONESIAN HERBAL INGREDIENTS

    OpenAIRE

    2015-01-01

    The present study was to aimed to evaluated and compare in vitro antioxidant activities of 2 Indonesian herbal ingredients (A and B), determined total phenol content., cytotoxic and apoptosis induction activities on HL-60 cells. These data were providing some useful information for people healthy dietary and the new potential application of natural antioxidant containing food materials in functional foods and also as new cancer therapeutics promising candidates. The parameters were total anti...

  1. Antioxidant Activities of Total Pigment Extract from Blackberries

    OpenAIRE

    Jiao, Zhonggao; Liu, Jiechao; Wang, Sixin

    2005-01-01

    Total pigment has been extracted from blackberries and its antioxidant activity against lipid peroxidation and scavenging capacities towards superoxide anion radicals, hydroxyl radicals and nitrite in different in vitro systems have been investigated. The total pigment extract from blackberries (TPEB) exhibited strong antioxidant activity against lipid peroxidation in a linoleic acid model system and scavenging capacities towards superoxide anion radicals, generated by a pyrogallol autoxidati...

  2. Antioxidant activity of selected wild Canadian prairie fruits

    OpenAIRE

    2015-01-01

    Background. Canadian prairies are a habitat for unique wild plants. The main object of the present study was to investigate phytochemicals content and antioxidant activity in seven wild Canadian prairie fruits. Material and methods. The presence of total phenolics, flavonoids, anthocyanins and antioxidant activity were identified in the extracts according to standard procedure. Results. Wild rose had the highest amounts of total phenolics and total flavonoids, whereas ...

  3. Pedalium murex Linn (Pedaliaceae) fruits:a comparative antioxidant activity of its different fractions

    Institute of Scientific and Technical Information of China (English)

    DK Patel; R Kumar; SK Prasad; S Hemalatha

    2011-01-01

    Objective: To examine the antioxidant activity and total phenolic content of different solvent fractions of Pedalium murex (P. murex) Linn fruits (Family: Pedaliaceae) as well as the correlation between the total antioxidant capacity and total phenolic content. Methods: In the present study, the antioxidant activities of P. murex were evaluated using six in-vitro assays, namely total antioxidant assay, DPPH assay, reducing power, nitric oxide scavenging, hydrogen peroxide scavenging and deoxyribose scavenging assays, and total phenol contents were also investigated.Results:The ethyl acetate (EA) fraction was found to have high levels of phenolic content (298.72±2.09 mg GAE/g). The EA fraction exhibit higher total antioxidant capacity, higher percentage of DPPH radical scavenging activity (135.11±2.95μg/mL), nitric oxide (200.57±4.51μg/mL), hydrogen peroxide (217.91±6.12 μg/mL), deoxyribose (250.01±4.68μg/mL) and higher reducing power. Correlation coefficient (r 2=0.914) was found to be significant between total phenolic content and total antioxidant activity. Conclusions: In general, the results indicate that the EA fractions are rich in phenolic antioxidants with potent free radical scavenging activity implying their importance to human health.

  4. Bioactive phytochemicals and antioxidant activity in fresh and dried lychee fractions1

    Directory of Open Access Journals (Sweden)

    Estela de Rezende Queiroz

    Full Text Available Fruit of the lychee cv. Bengal are approximately 50% peel and seeds, which are discarded. These by-products have antioxidant compounds which are capable of blocking the harmful effects of free radicals in the body. Bioactive compounds (ascorbic acid, beta-carotene, lycopene and phenols and antioxidant activity were evaluated in different extracts, both fresh and dried at 45 °C, of the skin, pulp and seeds of the lychee, which were subjected to principal component analysis to clarify which of the compounds are responsible for this activity. Principal component analysis explained 82.90% of the variance of the antioxidant profile of the lychee. The peel displayed higher levels of phenols, ascorbic acid, beta-carotene and antioxidant activity, while the seeds stood out due to their levels of lycopene. With drying, there was a decrease in the levels of ascorbic acid and beta-carotene and in antioxidant activity, with an increase in the levels of phenols and lycopene. The antioxidant activity found in the peel and seeds of the lychee is high, and is mainly due to ascorbic acid and beta-carotene, as demonstrated by principal component analysis, allowing the use of these fractions as sources of natural antioxidants.

  5. Antioxidative activities of hydrolysates from edible birds nest using enzymatic hydrolysis

    Science.gov (United States)

    Muhammad, Nurul Nadia; Babji, Abdul Salam; Ayub, Mohd Khan

    2015-09-01

    Edible bird's nest protein hydrolysates (EBN) were prepared via enzymatic hydrolysis to investigate its antioxidant activity. Two types of enzyme (alcalase and papain) were used in this study and EBN had been hydrolysed with different hydrolysis time (30, 60, 90 and 120 min). Antioxidant activities in EBN protein hydrolysate were measured using DPPH, ABTS+ and Reducing Power Assay. From this study, increased hydrolysis time from 30 min to 120 min contributed to higher DH, as shown by alcalase (40.59%) and papain (24.94%). For antioxidant assay, EBN hydrolysed with papain showed higher scavenging activity and reducing power ability compared to alcalase. The highest antioxidant activity for papain was at 120 min hydrolysis time with ABTS (54.245%), DPPH (49.78%) and Reducing Power (0.0680). Meanwhile for alcalase, the highest antioxidant activity was at 30 min hydrolysis time. Even though scavenging activity for EBN protein hydrolysates were high, the reducing power ability was quite low as compared to BHT and ascorbic Acid. This study showed that EBN protein hydrolysate with alcalase and papain treatments potentially exhibit high antioxidant activity which have not been reported before.

  6. Comparative study on antioxidant activity of different varieties of commonly consumed legumes in India.

    Science.gov (United States)

    Marathe, Sushama A; Rajalakshmi, V; Jamdar, Sahayog N; Sharma, Arun

    2011-09-01

    Legumes are rich source of proteins, dietary fiber, micronutrients and bioactive phytochemicals. Thirty different varieties of commonly consumed legumes in India, were screened for phenolic content and antioxidant activity using, radical scavenging [(1,1-diphenyl-2-picryl-hydrazyl (DPPH·) and 2,2'-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid, (ABTS·⁺], Ferric Reducing Antioxidant Power (FRAP) and metal ion (Fe²⁺) chelation assays. Legumes varied largely in their antioxidant activity. Horse gram, common beans, cowpea (brown and red) and fenugreek showed high DPPH· radical scavenging activity (>400 units/g), while lablab bean (cream and white), chickpea (cream and green), butter bean and pea (white and green) showed low antioxidant activity (legumes having dark color seed coat did not always possessed high antioxidant activity (e.g. moth bean, black pea, black gram, lentils). Antioxidant activity showed positive correlation (r²>0.95) with phenolic contents, in DPPH·, ABTS·⁺ and FRAP assays, whereas poor correlation (r²=0.297) was observed between Fe²⁺ chelating activity of the legumes and phenolic contents.

  7. Antioxidant and antimicrobial activities of selected medicinal plants from Algeria

    Directory of Open Access Journals (Sweden)

    Krimat Soumia

    2014-06-01

    Full Text Available Objective: To evaluate the antioxidant and antimicrobial activity of methanolic extract extracts of selected Algerian medicinal plants. Methods: Antioxidant activity of extracts was evaluated in terms of radical scavenging potential (2,2-diphenyl-1-picrylhydrazyl and β-carotene bleaching assay. Total phenolic contents and flavonoid contents were also measured. Antimicrobial activity of these plants was examined against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. Results: The values of IC50 ranged from 4.30 μg/mL to 486.6 μg/mL for the DPPH method, while total antioxidant activity using β-carotene/linoleic acid bleaching assay ranged from 17.03% to 86.13%. It was found that Pistacia lentiscus showed the highest antioxidant capacities using DPPH assay (IC50=4.30 μg/mL, while Populus trimula, Origanum glandulosum, Centaurea calcitrapa, Sysimbrium officinalis and Rhamnus alaternus showed the highest percent of total antioxidant activity in β-carotene/linoleic acid bleaching assay. Total phenolic and flavonoid contents ranged from 3.96 to 259.65 mg GAE/g extract and from 1.13 to 26.84 mg QE/g extract, respectively. The most interesting antimicrobial activity was obtained from Sysimbrium officinalis, Rhamnus alaternus, Origanum glandulosum, Cupressus sempervirens, Pinus halipensis and Centaurea calcitrapa. Conclusions: The results indicated that the plants tested may be potential sources for isolation of natural antioxidant and antimicrobial compounds.

  8. Antioxidant and antimicrobial activities of selected medicinal plants from Algeria

    Institute of Scientific and Technical Information of China (English)

    Krimat Soumia; Dob Tahar; Lamari Lynda; Boumeridja Saida; Chelghoum Chabane; Metidji Hafidha

    2014-01-01

    Objective:To evaluate the antioxidant and antimicrobial activity of methanolic extract extracts of selected Algerian medicinal plants. Methods:Antioxidant activity of extracts was evaluated in terms of radical scavenging potential (2,2-diphenyl-1-picrylhydrazyl) and β-carotene bleaching assay. Total phenolic contents and flavonoid contents were also measured. Antimicrobial activity of these plants was examined against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. Results:The values of IC50 ranged from 4.30 μg/mL to 486.6 μg/mL for the DPPH method, while total antioxidant activity using β-carotene/linoleic acid bleaching assay ranged from 17.03%to 86.13%. It was found that Pistacia lentiscus showed the highest antioxidant capacities using DPPH assay (IC50=4.30 μg/mL), while Populus trimula, Origanum glandulosum, Centaurea calcitrapa, Sysimbrium officinalis and Rhamnus alaternus showed the highest percent of total antioxidant activity inβ-carotene/linoleic acid bleaching assay. Total phenolic and flavonoid contents ranged from 3.96 to 259.65 mg GAE/g extract and from 1.13 to 26.84 mg QE/g extract, respectively. The most interesting antimicrobial activity was obtained from Sysimbrium officinalis, Rhamnus alaternus, Origanum glandulosum, Cupressus sempervirens, Pinus halipensis and Centaurea calcitrapa. Conclusions:The results indicated that the plants tested may be potential sources for isolation of natural antioxidant and antimicrobial compounds.

  9. Comparative assessment of antioxidant activity of chicory products

    Directory of Open Access Journals (Sweden)

    Z. A. Badretdinova

    2016-01-01

    Full Text Available There are various methods for determining the antioxidant activity. They are differing by the source of oxidation, oxidized compounds and methods for the measurement of oxidized compounds. Methods for determination of antioxidant activity provide a wide range of results, so the evaluation of the antioxidant activity should be carried out in several ways, and the results should be interpreted with caution. In assessing antioxidant capacity is necessary to consider not only the nature and content of the reducing agent in the test facility, but also the possibility of their mutual influence. The objects of research in this research work were Instant chicory (Leroux, Fried chicory (Leroux, Instant chicory (LLC Favorit, Instant chicory (LLC SlavKofe, Instant chicory with hawthorn (Iceberg Ltd and K, Instant chicory (LLC Flagistom, Instant chicory (Ltd. Around the World, Instant chicory (LLC Beta +, Chicory flour (Leroux, Chicory flour. Antioxidant activity of the products of chicory determined by spectrophotometric (using adrenaline and 2,2 – diphenyl- 1-picrylhydrazyl, and amperometric methods. These results indicate that chicory products possess antioxidant activity but not give fully correlated with each other results. The highest antioxidant activity have fried products from chicory – Instant chicory (LLC SlavKofe and Instant chicory with hawthorn (Iceberg Ltd and K. No fried products from chicory (Chicory flour (Leroux have lower antioxidant activity then fried products from chicory. Because during frying new compounds are formed – chikoreol, which comprises acetic and valeric acid, acrolein and furfural, furfuryl alcohol, diacetyl. Best convergence results provide spectrophotometric methods using epinephrine and 2,2 – diphenyl-1-picrylhydrazyl.

  10. Antioxidant activity of white grape seed extracts on DPPH radicals

    Directory of Open Access Journals (Sweden)

    Ćetković Gordana S.

    2009-01-01

    Full Text Available Composition and antioxidant activity of grape seed extract (GSE obtained from red grape varietes are very well documented, in contrast to the white varietes. This paper presents the results of polyphenols content of ethyl acetate extract of grape seeds, obtained from two white grape varieties, Italian Riesling and Župljanka, and their antioxidant activity on the stable DPPH radical. The influence of the addition of GSE to raspberry juice on the DPPH radical was also examined. Content of total polyphenols in GSEs ranged between 81.6 and 82.8% (w/w, and the contetn of flavan-3-ols between 66.2 and 91.0% (w/w. HPLC results showed that the most abundant components in the extract were (+-catechin and (--epicatechin for both grape varieties. All tested GSEs exhibited good antioxidant activity. IC50 values for the GSEs of Italian Riesling and Župljanka were 0.79 and 0.95 mg sample/mg DPPH radical, respectivelly. Since the GSE of Italian Riesling possesed stronger antioxidant activity, it was used for further experiments. The IC50 value for raspberry juice was 4.18 mg raspberry juice/mg DPPH. The raspberry juice with addition of 0.60 μg/mL of GSE showed antioxidant activity of 39.2%. The same juice with the threefold concentration of vitamin C (1.81 μg/ml exhibited similar antioxidant activity (33.9%. Antioxidant activity of the same amount of juice without added antioxidants was lower (15.7%. The results showed that the GSE of white varietes could be considered as a good functional food ingredient.

  11. Antioxidant Activity and α-Glucosidase Inhibitory Activities of the Polycondensate of Catechin with Glyoxylic Acid.

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    Sheng Geng

    Full Text Available In order to investigate polymeric flavonoids, the polycondensate of catechin with glyoxylic acid (PCG was prepared and its chemically antioxidant, cellular antioxidant (CAA and α-glucosidase inhibitory activities were evaluated. The DPPH and ABTS radical scavenging activities and antiproliferative effect of PCG were lower than those of catechin, while PCG had higher CAA activity than catechin. In addition, PCG had very high α-glucosidase inhibitory activities (IC50 value, 2.59 μg/mL in comparison to catechin (IC50 value, 239.27 μg/mL. Inhibition kinetics suggested that both PCG and catechin demonstrated a mixture of noncompetitive and anticompetitive inhibition. The enhanced CAA and α-glucosidase inhibitor activities of PCG could be due to catechin polymerization enhancing the binding capacity to the cellular membrane and enzymes.

  12. Antioxidation of Anthocyanins in Photosynthesis Under High Temperature Stress

    Institute of Scientific and Technical Information of China (English)

    Ling Shao; Zhan Shu; Shu-Lan Sun; Chang-Lian Peng; Xiao-Jing Wang; Zhi-Fang Lin

    2007-01-01

    Chlorophyll fluorescence and antioxidative capability in detached leaves of the wild type Arabidopsis thaliana L. ecotype Landsberg erecta (Ler) and three mutants deficient in anthocyanins biosynthesis (tt3, tt4, and tt3tt4) were investigated during treatment with temperatures ranging 25-45 ℃. In comparison with the wild type, chlorophyll fluorescence parameters Fv/Fm, ΦPSⅡ, electron transport rate (ETR), Fv/Fo and qP in three anthocyanin-deficient mutants showed a more rapidly decreasing rate when the temperature was over 35 ℃. Non-photochemical quenching (NPQ) in these mutants was almost completely lost at 44 ℃, whereas the content of heat stable protein dropped and the rate of the membrane leakage increased.Fo-temperature curves were obtained by monitoring Fo levels with gradually elevated temperatures from 22 ℃ to 72 ℃ at 0.5℃/min. The inflexion temperatures of Fo were 45.8 ℃ in Ler, 45.1 ℃ in tt3, 44.1 ℃ in tt4 and 42.3 ℃ in tt3tt4, respectively.The temperatures of maximal Fo in three mutants were 1.9-3.8 ℃ lower than the wild type plants. Meanwhile, three mutants had lower activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) and an inferior scavenging capability to DPPH (1.1-diphenyl-2-picrylhy.drazyl) radical under heat stress, and in particular tt3tt4 had the lowest antioxidative potential. The results of the diaminobenzidine-H2O2 histochemical staining showed that H2O2 was accumulated in the leaf vein and mesophyll cells of mutants under treatment at 40 ℃, and it was significantly presented in leaf cells of tt3tt4.The sensitivity of Arabidopsis anthocyanins-deficient mutants to high temperatures has revealed that anthocyanins in normal plants might provide protection from high temperature injury, by enhancing its antioxidative capability under high temperature stress.

  13. Plasma zinc antioxidant vitamins, glutathione levels and total antioxidant activity in oral leukoplakia

    Directory of Open Access Journals (Sweden)

    Subhash Chandra Bose

    2012-01-01

    Full Text Available Background: Leukoplakia is a common, potentially premalignant lesion with malignant transformation rate from 1 to 17% with highest transformation rate for the lesions on the floor of the mouth, soft palate and tongue. One of the main etiological factors is consuming areca nut and its commercial preparations which generate high levels of reactive oxygen species during their metabolism. So the aim of this present study is to evaluate the plasma levels of antioxidant vitamins, antioxidant mineral zinc, glutathione and total antioxidant status (TAS in leukoplakia patients. Materials and Methods: For this cross-sectional study, we selected 23 newly diagnosed oral leukoplakia patients of both sexes within the age group 28-40 years and the same number of age and sex matched healthy individuals without having history of any systemic illness were selected as control group. In both the groups, we measured plasma antioxidant vitamins A, C, E, antioxidant mineral zinc, GSH and TAS. Student′s t test was applied and the P value <0.001 was considered as statistically significant. Results: We observed very low levels of antioxidant vitamins A, C, E, antioxidant mineral zinc and antioxidant metabolite GSH (P<0.001 and at the same time we also observed very poor (TAS (P<0.001 in leukoplakia patients when compared to patients in control group. Conclusion: The consumption of tobacco or areca quid which contains high copper levels creates an oxidative stress like environment during their metabolism, might play a major role in causation and propagation of oral leukoplakia.

  14. New acylated anthocyanins from purple yam and their antioxidant activity.

    Science.gov (United States)

    Moriya, Chiemi; Hosoya, Takahiro; Agawa, Sayuri; Sugiyama, Yasumasa; Kozone, Ikuko; Shin-Ya, Kazuo; Terahara, Norihiko; Kumazawa, Shigenori

    2015-01-01

    Purple yam (Dioscorea alata L.), which is widely distributed in tropical and subtropical regions, is characterized by its color and viscosity. Previous studies have shown that purple yams contain a variety of acylated anthocyanins that exhibit higher levels of antioxidant activity than the corresponding nonacylated compounds. In this study, the pigments found in purple yams from the Philippines (D. alata) were isolated and evaluated in terms of antioxidant activity. Four new acylated anthocyanins, alanins (1-4) were isolated from the MeOH extracts of purple yam, which were subsequently determined to be cyanidin (1, 2, and 4) and peonidin (3) type compounds, along with four known anthocyanins (5-8). The structures of 1-4 were determined by spectroscopic methods, including NMR and MS analyses. The antioxidant activities of anthocyanins 1-8 were investigated using oxygen radical absorbing capacity and ferric reducing antioxidant power assays.

  15. Fruit juice drinks prevent endogenous antioxidant response to high-fat meal ingestion.

    Science.gov (United States)

    Miglio, Cristiana; Peluso, Ilaria; Raguzzini, Anna; Villaño, Deborah V; Cesqui, Eleonora; Catasta, Giovina; Toti, Elisabetta; Serafini, Mauro

    2014-01-28

    High-fat meals (HFM) induce metabolic stress, leading to the activation of protective mechanisms, including inflammation and endogenous antioxidant defences. In the present study, we investigated the effects of antioxidant-rich fruit juice drinks on the endogenous antioxidant response induced by HFM. In a double-blind, cross-over design (10 d washout), fourteen overweight volunteers were randomly assigned to one of the following interventions: HFM+500 ml placebo beverage (HFM-PB, free from fruit); HFM+500 ml antioxidant beverage 1 (HFM-AB1; apple, grape, blueberry and pomegranate juices and grape skin, grape seed and green tea extracts); HFM+500 ml antioxidant beverage 2 (HFM-AB2; pineapple, black currant and plum juices). HFM-PB consumption increased the plasma levels of thiols (SH) (4 h, Pfruit juice drinks.

  16. Seasonal Variation, Chemical Composition and Antioxidant Activity of Brazilian Propolis Samples

    Directory of Open Access Journals (Sweden)

    Érica Weinstein Teixeira

    2010-01-01

    Full Text Available Total phenolic contents, antioxidant activity and chemical composition of propolis samples from three localities of Minas Gerais state (southeast Brazil were determined. Total phenolic contents were determined by the Folin–Ciocalteau method, antioxidant activity was evaluated by DPPH, using BHT as reference, and chemical composition was analyzed by GC/MS. Propolis from Itapecerica and Paula Cândido municipalities were found to have high phenolic contents and pronounced antioxidant activity. From these extracts, 40 substances were identified, among them were simple phenylpropanoids, prenylated phenylpropanoids, sesqui- and diterpenoids. Quantitatively, the main constituent of both samples was allyl-3-prenylcinnamic acid. A sample from Virginópolis municipality had no detectable phenolic substances and contained mainly triterpenoids, the main constituents being α- and β-amyrins. Methanolic extracts from Itapecerica and Paula Cândido exhibited pronounced scavenging activity towards DPPH, indistinguishable from BHT activity. However, extracts from Virginópolis sample exhibited no antioxidant activity. Total phenolic substances, GC/MS analyses and antioxidant activity of samples from Itapecerica collected monthly over a period of 1 year revealed considerable variation. No correlation was observed between antioxidant activity and either total phenolic contents or contents of artepillin C and other phenolic substances, as assayed by CG/MS analysis.

  17. Antioxidant and antiproliferative activities of Helleborus odorus Waldst. & Kit, H. multifidus Vis. and H. hercegovinus Martinis.

    Science.gov (United States)

    Cakar, Jasmina; Parić, Adisa; Vidic, Danijela; Haverić, Anja; Haverić, Sanin; Maksimović, Milka; Bajrović, Kasim

    2011-12-01

    This study was undertaken in order to evaluate possible antioxidative and antiproliferative activities of three Helleborus taxa. The dry leaves and roots of three Helleborus taxa were extracted with ethanol and water. A phytochemical evaluation of the selected extracts was performed using spectrophotometric methods and a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay was used for measuring the antioxidative activity of extracts. The antiproliferative activity of the three Helleborus taxa was studied using Burkitt's lymphoma B cells (BJAB) cell lines. The phytochemical evaluation showed that the leaves contain high levels of total phenolic and flavonoid content. Results from the DPPH assay indicated that the activity of the ethanol and water extracts of the leaves was higher than that of positive control (thymol). Extracts from the roots of H. odorus also displayed higher antioxidant activity than the positive probe, while H. mulifidus and H. hercegovinus root extracts were less effective. A statistically significant correlation between total phenolic content and antioxidative properties indicates that these compounds contribute to the antioxidant activity. The highest percentage of cell growth inhibition was observed when testing the water root extracts of H. multifidus (50.14%) and H. hercegovinus (49.04%). In contrast, the water leaf extract of H. hercegovinus exhibited the lowest inhibition of cell growth (8.59%), although it showed strong antioxidant activity.

  18. Phenolic composition and antioxidant activity of different parts of pistacia vera L.

    Directory of Open Access Journals (Sweden)

    Raouia Boumaiza

    2016-09-01

    Full Text Available Phenolic compounds are widely distributed in almost all higher plants and are present in different tissues. The quality and quantity of phenolics are used as criteria for variety identification. The present study has been planned to determine the phenolic composition of Pistacia vera.Total phenolic content and concentration of flavonoids of eight different extracts, from the floral buds and leaves of Pistacia vera. (Anacardiaceae were determined using spectrophotometric methods. The total phenolic content ranged from 401.64 to 513.77 (mg GAE/g DW. The total flavonoid concentrations varied from 8.28 to 67.94 mg CE/g.Antioxidant activity was assessed by three methods: DPPH°+, β-carotene and ABTS and determined respectively as IC50 (mg/mL, CAA and PI (%, all extract have shown to be endowed by a high antioxidant activity similar to that of BHT.Ethanolic extract of male floral buds showed the highest phenolic and flavonoid concentration and the strongest antioxidant activity. The significant linear correlation was confirmed between the values for the total phenolic content and antioxidant activity of plant extracts.Phenolic compounds were investigated by LTQ Orbitrap LC-MS analysis and revealed 17compounds split into 5 groups: Abscisic acids, phenolic acids and their derivatives, flavonols, flavanones, flavones, flavanols. The high contents of phenolic compounds indicated that these last contribute to the antioxidant activity. The Pistacia vera can be regarded as promising candidates for natural plant sources of antioxidants with high value.

  19. Evaluation of in vitro antioxidant and anti-inflmmatory activities of Ximenia americana extracts

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    Arun Kashivishwanath Shettar

    2015-11-01

    Full Text Available Objective: To evaluate in vitro antioxidant and anti-inflammatory activity of Ximenia americana extracts. Methods: Herbal extraction was done by Soxhlet extraction method with increasing polarity of solvents viz., chloroform, ethyl acetate, methanol, ethanol and water. Phytochemical analysis was done using different biochemical tests. Antioxidant potential of plant extracts were analyzed by ferric ion reducing antioxidant power, phosphomolybdenum and 2,2-diphenyl-1- picrylhydrazyl, and anti-inflammatory activity by using protein denaturation in vitro bioassay. Total phenolic content of each extract was also determined to assess their corresponding effect on antioxidant capacity of plant. Results: Phytochemical analysis showed that each solvent extract contained broad spectrum of secondary metabolites, phenolic compounds, flavonoids, tannins and glycosides, whereas compared to other solvent extracts, chloroform extract showed negative result for phenolic compounds whereas aqueous extract exhibited the highest phenolic content and the significant antioxidant capacity based on the test performed. Out of all extracts, methanol extract showed high anti-inflammatory activity. Conclusions: The present study revealed that different solvent extracts of Ximenia americana leaves contain broad spectrum of bioactive compounds. Results confirm that aqueous extract exhibited high antioxidant activity and methanol extract exhibited high antiinflammatory activity. Further study requires purification, characterization and structural elucidation of phenolic compounds in both extracts that may help in the development of new phytopharmaceuticals.

  20. Antioxidant and antiproliferative activities of Desmodium triflorum (L.) DC.

    Science.gov (United States)

    Lai, Shang-Chih; Ho, Yu-Ling; Huang, Shun-Chieh; Huang, Tai-Hung; Lai, Zhen-Rung; Wu, Chi-Rei; Lian, Kuo-Yuan; Chang, Yuan-Shiun

    2010-01-01

    This study evaluated the antioxidant and antiproliferative activities of the crude extract and fractions of Desmodium triflorum (L.) DC. The total phenolic content, 1,1-diphenyl-2- picrylhydrazyl hydrate (DPPH) free radical scavenging activity, trolox equivalent antioxidant capacity (TEAC), reducing power, total flavonoid content of D. triflorum were evaluated for the exploration of its antioxidant activities. Furthermore, its antiproliferative activities were investigated through the MTT method. It was compared with the antioxidant capacities of known antioxidants, including catechin, alpha-tocopherol, trolox and ascorbic acid. Among all fractions, ethyl acetate fraction was the most active in scavenging DPPH and TEAC radicals, of which 0.4 mg was equivalent to 186.6 +/- 2.5 microg and 82.5 +/- 2.1 microg of alpha-tocopherol and trolox respectively. The total phenolic and flavonoid contents of the crude extract were equivalent to 36.60 +/- 0.1 mg catechin and 45.6 +/- 0.6 mg rutin per gram respectively. In the reducing power assay, 1.25 mg of crude extract was similar to 61.2 +/- 0.3 microg of ascorbic acid. For the assessment of the safety and toxicity of D. triflorum, LD(50) of the crude extract was greater than 10 g/kg when administered to mice through gastric intubation. The above experimental data indicated that D. triflorum was a potent antioxidant medicinal plant, and such efficacy may be mainly attributed to its polyphenolic compounds.

  1. Antioxidant and antibacterial activity of different parts of Leucas aspera

    Institute of Scientific and Technical Information of China (English)

    Ai Lan Chew; Jeyanthi James Antony Jessica; Sreenivasan Sasidharan

    2012-01-01

    Objective: To evaluate antioxidant, antimicrobial and cytotoxic activity of different parts (root, flower, leaf and stem) of Leucas aspera (L. aspera) (Labiatae). Methods: Different parts of L. aspera were extracted with 80% (v/v) methanol. The methanol extracts were subjected to antioxidant, antimicrobial and brine shrimp lethality assay. Results: All the extracts showed moderate to potent antioxidant activity, among which the root extract demonstrated the strongest antioxidant activity with the IC50 value of 6.552 μg/mL. Methanol extract of root possessed antioxidant activity near the range of vitamin E and thus could be a potential rich source of natural antioxidant. In case of antimicrobial screening, crude extracts of root, flower, leaf and stem showed notable antibacterial activity against tested microorganisms. The root extract showed the highest mean zone of inhibition ranging from 9.0-11.0 mm against tested microorganisms, at a concentration of 100 mg/mL. In the brine shrimp lethality bioassay, it was evident that the methanol root extract did not show significant toxicity. The LC50 value for 12 h and 24 h observation was 2.890 mg/mL and 1.417 mg/mL, respectively. Conclusions: The present finding suggests that the methanol root extract of L. aspera could be developed as pharmaceutical products.

  2. Antioxidant activity of gilan Mentha pulegium during growth.

    Science.gov (United States)

    Shahmohamadi, Rezvan; Sariri, Reyhaneh; Rasa, Mahdi; Aghamali, Mahmudreza

    2014-02-01

    Antioxidant activity and total phenolic contents of methanolic and hydroalcoholic-acetone extracts of Iranian Mentha pulegium in two stages of maturity were investigated. The aim was to investigate the most suitable solvent for extraction of antioxidants and to find the correlation existed between plant growth stage and its antioxidant capacity. In vitro antioxidant properties of the extracts were examined by 1, 1 diphenyl-2-picrylhydrazyl (DPPH) and Ferric Reducing/Antioxidant Power (FRAP) methods. Moreover, total phenolic contents were determined by the Folin-Ciocalteu method. On the other hand, the phenolic compounds were analyzed by chromatographic methods, TLC and SPE-reversed phase-HPLC. The results revealed that the antioxidant capacities and total phenol contents of the extracts in flowering season were higher than pre-flowering season. A positive relationship was found between the results obtained from three different assay methods used, i.e. FRAP, DPPH and phenol contents. The TLC chromatogram of the two extracts showed differences in the number of separated compounds of extracts. HPLC results indicated that the fraction collected with washing buffer (pH = 6) had highest antioxidant activity.

  3. Effect of Freeze-Drying on the Antioxidant Compounds and Antioxidant Activity of Selected Tropical Fruits

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    Muhammad Redzuan Hairuddin

    2011-07-01

    Full Text Available The effects of freeze-drying on antioxidant compounds and antioxidant activity of five tropical fruits, namely starfruit (Averrhoa carambola L., mango (Mangifera indica L., papaya (Carica papaya L., muskmelon (Cucumis melo L., and watermelon Citruluss lanatus (Thunb. were investigated. Significant (p < 0.05 differences, for the amounts of total phenolic compounds (TPC, were found between the fresh and freeze-dried fruit samples, except muskmelon. There was no significant (p > 0.05 change, however, observed in the ascorbic acid content of the fresh and freeze-dried fruits. Similarly, freeze-drying did not exert any considerable effect on β-carotene concentration of fruits, except for mango and watermelon, where significantly (p < 0.05 higher levels were detected in the fresh samples. The results of DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging and reducing power assays revealed that fresh samples of starfruit and mango had relatively higher antioxidant activity. In case of linoleic acid peroxidation inhibition measurement, a significant (p < 0.05 but random variation was recorded between the fresh and freeze-dried fruits. Overall, in comparison to β-carotene and ascorbic acid, a good correlation was established between the result of TPC and antioxidant assays, indicating that phenolics might have been the dominant compounds contributing towards the antioxidant activity of the fruits tested.

  4. In vitro antioxidant activities of edible artichoke (Cynara scolymus L.) and effect on biomarkers of antioxidants in rats

    DEFF Research Database (Denmark)

    Jimenez-Escrig, A.; Dragsted, Lars Ove; Daneshvar, Bahram;

    2003-01-01

    Artichoke (Cynara scolymus L.), an edible vegetable from the Mediterranean area, is a good source of natural antioxidants such as vitamin C, hydroxycinnamic acids, and flavones. The antioxidant activity of aqueous-organic extracts of artichoke were determined using three methods: (a) free radical 2......,2-diphenyl-1-picrylhydrazyl (DPPH.) scavenging, (b) ferric-reducing antioxidant power (FRAP), and...

  5. Stabilization of red fruit-based smoothies by high-pressure processing. Part A. Effects on microbial growth, enzyme activity, antioxidant capacity and physical stability.

    Science.gov (United States)

    Hurtado, Adriana; Guàrdia, Maria Dolors; Picouet, Pierre; Jofré, Anna; Ros, José María; Bañón, Sancho

    2017-02-01

    Non-thermal pasteurization by high-pressure processing (HPP) is increasingly replacing thermal processing (TP) to maintain the properties of fresh fruit products. However, most of the research on HPP-fruit products only partially addresses fruit-pressure interaction, which limits its practical interest. The objective of this study was to assess the use of a mild HPP treatment to stabilize red fruit-based smoothies (microbial, enzymatic, oxidative and physical stability). HPP (350 MPa/10 °C/5 min) was slightly less effective than TP (85 °C/7 min) in inactivating microbes (mesophilic and psychrophilic bacteria, coliforms, yeasts and moulds) in smoothies kept at 4 °C for up to 28 days. The main limitation of using HPP was its low efficacy in inactivating oxidative (polyphenol oxidase and peroxidase) and hydrolytic (pectin methyl esterase) enzymes. Data on antioxidant status, colour parameters, browning index, transmittance, turbidity and viscosity confirmed that the HPP-smoothies have a greater tendency towards oxidation and clarification, which might lead to undesirable sensory and nutritional changes (see Part B). The microbial quality of smoothies was adequately controlled by mild HPP treatment without affecting their physical-chemical characteristics; however, oxidative and hydrolytic enzymes are highly pressure-resistant, which suggests that additional strategies should be used to stabilize smoothies. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  6. Antioxidants

    Science.gov (United States)

    Antioxidants are man-made or natural substances that may prevent or delay some types of cell damage. Antioxidants are found in many foods, including fruits and ... are also available as dietary supplements. Examples of antioxidants include Beta-carotene Lutein Lycopene Selenium Vitamin A ...

  7. Antioxidant activities of bark extract from mangroves, Bruguiera cylindrica (L. Blume and Ceriops decandra Perr

    Directory of Open Access Journals (Sweden)

    M Krishnamoorthy

    2011-01-01

    Full Text Available Objectives : The antioxidant activities of two Indian mangrove plants, Bruguiera cylindrica and Ceriops decandra, were investigated. Materials and Methods : Total phenolics and total flavonoid contents of the mangroves were determined using folin-ciocalteu reagent method and aluminium chloride method, respectively. Antioxidant capacity was assessed by the following methods: 1,1-diphenyl-2-picryl hydroxyl (DPPH. quenching assay; 2,2′- azinobis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS. + cation decolorization test; scavenging capacity towards hydroxyl ion radicals (.OH; reductive capacity; and antihemolytic activity. Results : The mangroves yielded 233.3 ± 0.062 and 283.31 ± 0.04 mg gallic acid equivalent/g phenolic contents and 11.6 ± 0.12 and 15.1 ± 0.02 mg quercetin equivalent/g flavonoid contents. The methanol extracts of both mangroves exhibited high antiradical activity against DPPH., ABTS. + , and .OH radicals. The reductive capacity of the extracts increased with increasing concentration of samples. The extracts also inhibited H 2 O 2 induced hemolysis in cow blood erythrocytes. The antioxidant activities were found stronger than that of the reference standard, butylated hydroxy toluene (BHT. The antioxidant activity of mangrove plants was correlated with total phenolics and flavonoid contents. Conclusion : Both plants can be considered as good sources of natural antioxidants for medicinal uses. Further studies are necessary to isolate active principles responsible for the overall antioxidant activity of the extracts.

  8. Altered Activities of Antioxidant Enzymes in Patients with Metabolic Syndrome

    Directory of Open Access Journals (Sweden)

    Lucie Vávrová

    2013-02-01

    Full Text Available Objective: In the pathogenesis of the metabolic syndrome (MetS, an increase of oxidative stress could play an important role which is closely linked with insulin resistance, endothelial dysfunction, and chronic inflammation. The aim of our study was to assess several parameters of the antioxidant status in MetS. Methods: 40 subjects with MetS and 40 age- and sex-matched volunteers without MetS were examined for activities of superoxide dismutase (CuZnSOD, catalase (CAT, glutathione peroxidase 1 (GPX1, glutathione reductase (GR, paraoxonase1 (PON1, concentrations of reduced glutathione (GSH, and conjugated dienes in low-density lipoprotein (CD-LDL. Results: Subjects with MetS had higher activities of CuZnSOD (p Conclusions: Our results implicated an increased oxidative stress in MetS and a decreased antioxidative defense that correlated with some laboratory (triglycerides, high-density lipoprotein cholesterol (HDL-C and clinical (waist circumference, blood pressure components of MetS.

  9. Antioxidant activity of proanthocyanidins from adansonia digitata fruit

    Institute of Scientific and Technical Information of China (English)

    Abdelaaty AShahat; Hanaa HAhmed; Rasmeia AHassan; Ahmed AHussein

    2008-01-01

    Besides (-)-epicatechin,epicatechin-(4β-8)-epicatechin (procyanidin B2),epicatechin-(4β-6 )-epicate-chin (procyanidin B5),epicatechin-(4β-8,2β-O-7)-epicatechin (proanthocyanidin A2)and epicatechin-(4β-8)-epicatechin-(4β-8)-epicatechin (procyanidin C1),which were isolated before from Adansonia digita-ta,in this work an A-type proanthocyanidin trimer,i.e.epicatechin-(4β-8)-epicatechin-(4β-8,2β-O-7)-epicatechin,tetrameric procyanidin D1,i.e.epicatechin-(4β-8)-epicatechin-(4β-8)-epicatechin-(4β-8)-epicatechin and a polymeric compound were isolated from the pericarp (fruit wall)of the fruits for the first time from this plant .The antioxidant activity of different fractions and pure compounds was experimentally e-valuated in the DPPH - assay.The ethyl acetate fraction,and most of the isolated compounds displayed a high activity (IC50 2.40-9.60 μg/ml)compared with the reference antioxidant Trolox (IC50 12.18 μg/ml)as a standard.

  10. Antioxidant and Cytotoxic Activity of Phyllanthus acidus Fruit Extracts

    Science.gov (United States)

    Andrianto, D.; Widianti, W.; Bintang, M.

    2017-03-01

    Phyllanthus acidus is an Indonesian plant belonging to the Euphorbiaccae family. Extraction of P. acidus was performed using the maceration method. Four-solvent extraction process by ethanol, 70% ethanol, 30% ethanol, and water was used. The antioxidant activity from this extract was determined using 2,2-diphenyl-1-pikrilhidrazil (DPPH) and cytotoxicity (biological potency from this extract) using Brine Shrimp Lethality Test (BSLT) method. We found that the extraction yields of samples ranging from 1.13% to 20.25%. The ethanol extract showed the highest yield, while the lowest yield was reported in the water extract of P. acidus fruit. Among the samples, water extract of P. acidus exhibited high antioxidant activity with IC50 26.06 μg/mL. LC50 values for BSLT ranging from 473.26 to 908.98 μg/mL, with the water extract having the lowest value and therefore the most potent, and the ethanol extract having the highest value

  11. Identification and Evaluation of Antioxidant Activities of Bamboo Extracts

    Institute of Scientific and Technical Information of China (English)

    Mu Jun; Uehara Tohru; Li Jianzhang; Furuno Takeshi

    2004-01-01

    The antioxidant activity of solvent extracts from two main bamboo species, moso bamboo (Phyllostachys pubescens) and madake bamboo (P. Bambusoides) in Japan, was first evaluated by scavenging free radical of 1,1-diphenyl-2-picrylhydrazyl (DPPH), the inhibition activity for peroxidation of linoleic acid, and the reduction power. The methanol-extracts of moso bamboo culms and madake bamboo leaves presented stronger antioxidant activity compared with DPPH scavenging activity. Methanol-extract of moso bamboo culms was further fractionated by different solvents and n-butanol soluble fraction exhibited the most significant activity in the DPPH scavenging assay. The fractionation of n-butanol soluble extract was isolated by silica gel column with gradient mixture solvent of chloroform and methanol. The isolated fractions were directed by the antioxidant activity measured by scavenging the stable DPPH free radical. It was observed that most of the eluted fractions showed the antioxidative activity. Fractions acquired from elution with the mixture solvent of chloroform and methanol (10:1-5:1) showed stronger antioxidant activity than the other fractions.

  12. A Review of Pterostilbene Antioxidant Activity and Disease Modification

    Directory of Open Access Journals (Sweden)

    Denise McCormack

    2013-01-01

    Full Text Available Pterostilbene (trans-3,5-dimethoxy-4-hydroxystilbene is a natural dietary compound and the primary antioxidant component of blueberries. It has increased bioavailability in comparison to other stilbene compounds, which may enhance its dietary benefit and possibly contribute to a valuable clinical effect. Multiple studies have demonstrated the antioxidant activity of pterostilbene in both in vitro and in vivo models illustrating both preventative and therapeutic benefits. The antioxidant activity of pterostilbene has been implicated in anticarcinogenesis, modulation of neurological disease, anti-inflammation, attenuation of vascular disease, and amelioration of diabetes. In this review, we explore the antioxidant properties of pterostilbene and its relationship to common disease pathways and give a summary of the clinical potential of pterostilbene in the prevention and treatment of various medical conditions.

  13. Evaluation of antioxidant activity of vetiver (Vetiveria zizanioides L.) oil and identification of its antioxidant constituents.

    Science.gov (United States)

    Kim, Hyun-Jin; Chen, Feng; Wang, Xi; Chung, Hau Yin; Jin, Zhengyu

    2005-10-05

    Antioxidant capacities of vetiver (Vetiveria zizanioides) oil were evaluated by two different in vitro assays: the DPPH* free radical scavenging assay and the Fe2+-metal chelating assay. Results showed that the vetiver oil (VO) possessed a strong free radical scavenging activity when compared to standard antioxidants such as butylated hydroxytoluene (BHT) and alpha-tocopherol. However, its metal chelating capacity was relatively weak. VO (10 microL/mL) dissolved in methanol exhibited approximately 93% free radical scavenging activity in the DPPH* assay and approximately 34% Fe2+ chelating activity in the metal chelating assay. By contrast, 10 mM BHT and 0.1 mM alpha-tocopherol exhibited 93 and 89% free radical scavenging activities in the DPPH* assay, respectively, and 1 mM EDTA exhibited approximately 97% activity in the metal chelating assay. Among the complex constituents in the crude VO, beta-vetivenene, beta-vetivone, and alpha-vetivone, which had shown strong antioxidant activities, were isolated and identified using various chromatographic techniques including silica gel open column chromatography, silica HPLC, and GC-MS. These results show that VO and some of its inherent components can be potential alternative natural antioxidants.

  14. Study on the antioxidant activity and membrane interaction of a multiple antioxidant

    DEFF Research Database (Denmark)

    Jodko-Jodko-Piórecka, Kasia; Klösgen, Beate; Litwinienko, Grzegorz

    Catecholamines, including dopamine, have a role in the transduction of nervous stimuli. Apart from that, they might behave as endogenous phenolic antioxidants protecting the neuronal tissue from deleterious effects of oxidative stress. Results from our preliminary study1 even indicate a synergistic...... kinetic data that, as a prerequisite, verify the antioxidant activity of dopamine by measuring its scavenging activity against peroxyl radicals in a heterogeneous lipid/water system and give evidence about the synergistic effect between dopamine and PMHC. We also present preliminary results from ongoing....../PMHC antioxidative co-operation we propose that dopamine acts mainly as a scavenger of radicals near the membrane surface while PMHC is scavenging the peroxyl radicals within the membrane. References 1) Jodko, K.; Kowalewska, E.; Sikora, B.; Litwinienko, G. Free Radic. Res. 2009, 43, 58 2) Liu, J; Mori, A. Arch...

  15. Abortifacient and antioxidant activities of different extracts of Musa rosacea

    Institute of Scientific and Technical Information of China (English)

    M. Srikanth; T. Rajananda Swamy; T. Mallikarjuna Rao; B. Ganga Rao

    2013-01-01

    Objective: To evaluate abortifacient and antioxidant activity of Musa rosacea (M. rosacea).Methods:Abortifacient activity was evaluated in rats, compared with standard drug (Mifepristone) and antioxidant activity was evaluated by using three free radicals (Superoxide, Hydroxyl and DPPH). Results: The extracts showed preimplantation loss, postimplantation loss of implantations and decreased the survival ration of foetuses. Among all extracts hydroalcoholic extract showed better activity. The selected plant extracts showed concentration dependent percentage inhibition of free radicals. Among four extracts hydroalcoholic extract showed better activity with IC50 values on superoxide, hydroxyl and DPPH radicals were 180 µg, 218 µg and 116 µg. Conclusion:From the results obtained during the study it could be concluded that M. rosacea extracts have abortifacient and antioxidant components and the results support its folklore usage as abortifacient plant. Further is necessary for isolation and characterization of bioactive molecules which are responsible for these activities.

  16. Development of microalgae biomaterials with enhanced antioxidant activity using electron beam

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Younghwa; Park, Hyunjin; Choi, Soojeong; Lee, Jaehwa [Silla Univ., Busan (Korea, Republic of)

    2013-07-01

    By increasing the antioxidant products (e. g. antioxidant enzyme, carotenoid, phycobiliproteins, chlorophyll, lipid phenolic compounds, etc.) in microalgae, it could be useful for industry. In this study, mutants of fresh water microalgae Arthrospira platensis (A. platensis) by high energy electron beam were isolated and characterized. Those selected mutants showed higher growth rate than parental strain. The antioxidant enzyme activity (SOD and POD), flavonoid, phenolic compound and phycocyanin of mutants were increased about 2 times compared to wild type. Moreover, DPPH radical scavenging activity was increased about 20%. Microalgae species with improved growth rate and enhanced active compounds make the commercial process more feasible in industry. Using microalgae mutants with increased antioxidant products, it is useful to develop microalgae biomaterials for neutraceuticals.

  17. Activity-guided screening of the antioxidants from Paulownia tomentosa var. tomentosa Bark

    Directory of Open Access Journals (Sweden)

    Chuan-Ling Si

    2013-02-01

    Full Text Available Tree barks, as a type of forestry residues, are a rich and renewable bioresource that can produce high value-added products. Paulownia tomentosa var. tomentosa (PTT has been extensively used in traditional Chinese medicine to cure various diseases. However, the antioxidative activity of the chemical constituents of the tree has not yet been investigated. In this study, the bark of PTT were extracted and fractioned. Then the resulting ethyl acetate (EtOAc soluble fraction, which exhibited the strongest antioxidative effect, was subjected to repeated open column chromatography for purification. The screening process was carried out under the guidance antioxidative activity via diphenylpicrylhydrazyl (DPPH radical scavenging assay. Eight phenolic compounds, glucodistylin (I, luteolin (II, ellagic acid (III, cistanoside F (IV, campneoside II (V, isocampneoside II (VI, verbascoside (VII, and isoverbascoside (VIII, were isolated and their structures were elucidated by various spectroscopic analyses. Among the phenolics, II~VIII showed significant antioxidative activity.

  18. Bioactive content, hepatoprotective and antioxidant activities of ...

    African Journals Online (AJOL)

    Results: RP-HPLC analysis revealed high contents of quercitrin, rosmarinic and ferulic acid. ... marked antitumor activities against human colon ... aluminum chloride method, using quercetin as .... Injecting carbon tetrachloride to the animals pretreated with the plant extract at ... known that hepatotoxicity involves induction of.

  19. Sulforaphane composition, cytotoxic and antioxidant activity of crucifer vegetables

    Directory of Open Access Journals (Sweden)

    Mohamed A. Farag

    2010-01-01

    Full Text Available Sulphur compounds in sulphur rich food have been shown to significantly reduce the risk of cancer development. One such compound is sulforaphane (SF, a cancer chemopreventive agent identified in broccoli (F. cruciferae. In this study, SF content was assessed in extracts of several crucifer vegetables including broccoli, brussels sprout, green cabbage, red cabbage, Chinese kale and turnip, in parallel with anticancer and antioxidant activity. Among tested crucifers, cabbage demonstrated a pronounced anticancer effect against A-549 lung cancer cells, with an IC50 value of 38 μg mL−1, and correlated with high SF levels at 540 μg g−1. Except for red cabbage and kale, crucifer extracts displayed moderate to weak activity in scavenging 2,2-diphenyl-1-picrylhydrazyl (DDPH free radicals relative to vitamin E standard.

  20. Antioxidant activity of herbal polysaccharides and cough reflex.

    Science.gov (United States)

    Nosalova, G; Jurecek, L; Hromadkova, Z; Kostalova, Z; Sadlonova, V

    2013-01-01

    The extraction of Fallopia sachalinensis leaves resulted in two fractions (FS-1 and FS-2). Chemical and spectral analyses of samples revealed the prevalence of pectic polysaccharides with high galacturonic acid, arabinose, galactose, and rhamnose content. Arabinogalactan with a higher content of phenolic prevailed in the FS-1, whereas rhamnogalacturonan predominated in the FS-2 fraction. Both polysaccharides showed significant antioxidant activity according to DPPH and FRAP assays. Evaluation of antitussive activity in healthy adult conscious guinea pigs after oral application of 50 and 75 mg/kg of the FS-2 polysaccharide extracts showed a significant suppression of cough reflex, without an influence on specific airway resistance. The suppression of cough was comparable with that of codeine.

  1. Alpha-fetoprotein, identified as a novel marker for the antioxidant effect of placental extract, exhibits synergistic antioxidant activity in the presence of estradiol.

    Directory of Open Access Journals (Sweden)

    Hye Yeon Choi

    Full Text Available Placenta, as a reservoir of nutrients, has been widely used in medical and cosmetic materials. Here, we focused on the antioxidant properties of placental extract and attempted to isolate and identify the main antioxidant factors. Porcine placental extracts were prepared through homogenization or acid hydrolysis, and their antioxidant activity was investigated in the human keratinocyte HaCaT cell line. Treatment with homogenized placental extract (H-PE increased the cell viability of H2O2-treated HaCaT cells more than two-fold. H-PE treatment suppressed H2O2-induced apoptotic and necrotic cell death and decreased intracellular ROS levels in H2O2-treated HaCaT cells. The antioxidant factors in H-PE were found to be thermo-unstable and were thus expected to include proteins. The candidate antioxidant proteins were fractionated with cation-exchange, anion-exchange, and size-exclusion chromatography, and the antioxidant properties of the chromatographic fractions were investigated. We obtained specific antioxidant fractions that suppressed ROS generation and ROS-induced DNA strand breaks. From silver staining and MALDI-TOF analyses, alpha-fetoprotein (AFP precursor was identified as a main marker for the antioxidant effect of H-PE. Purified AFP or ectopically expressed AFP exhibited synergistic antioxidant activity in the presence of estradiol. Taken together, our data suggest that AFP, a serum glycoprotein produced at high levels during fetal development, is a novel marker protein for the antioxidant effect of the placenta that exhibits synergistic antioxidant activity in the presence of estradiol.

  2. ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC AND FLAVONOID CONTENT OF MORINDA CITRIFOLIA FRUIT EXTRACTS FROM VARIOUS EXTRACTION PROCESSES

    Directory of Open Access Journals (Sweden)

    PRAVEEN K. RAMAMOORTHY

    2007-04-01

    Full Text Available Soxhlet, Ultrasonic extract of Morinda citrifolia L. fruit and four extracts from high pressure extraction at 10 MPa using ethanol, ethyl acetate as solvent and dried by vacuum oven and spray dryer were analyzed for their antioxidant activity by peroxide value method and diphenylpicrylhydrazyl radical scavenging method. The five extracts along with the reference samples, butylated hydroxyl toluene and tannic acid were further analyzed to determine their total phenolic content by Folin-Ciocalteau method and total flavonoid content by Dowd method. The M. citrifolia extract by high pressure extraction with ethyl acetate as solvent and spray dried was found to exhibit highest antioxidant activity and total flavonoid content. High total phenolic content was determined in the high pressure extract using ethyl acetate as solvent and vacuum dried. It was interesting to note that ultrasonic extract exhibited significant antioxidant activity, total phenolic and flavonoid content. High pressure extracted M. citrifolia in ethanol was found to express lesser values comparatively. The significant difference in activity among the high pressure extracts was found to be due to the polarity of the solvents used for extraction as M. citrifolia fruit contains relatively larger quantity of non-polar antioxidant compounds. It was also found that the drying methods had significant impact on the antioxidant activity, total phenolic and flavonoid content of the extracts.

  3. In vitro antioxidant potential and free radical scavenging activity of various extracts of pollen of Typha domigensis Pers.

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    Sardar, Andleeb Anwar; Akhan, Zaheer-ud-Din; Perveen, Anjum; Farid, Sidra; Khan, Islamullah

    2014-03-01

    Antioxidant potential of the pollen of Typha domigensis Pers. using Ferric Reducing ower, Metal Chelating Activity and Trolox Equivalent Antioxidant Capacity (TEAC) assays has been carried out in the current research work. The antioxidant components were initially extracted from the pollen in methanol and were further fractionated in solvents of different polarity such as n-Hexane, Chloroform, Ethyl Acetate and Water. Methanol extract which was found to have high reducing power, total phenolic contents with high metal chelating activity, has considerable prospective to utilize as a natural antioxidant and be capable to link with the total phenolic contents of plant.

  4. Evaluation of antioxidant activity of two important memory enhancing medicinal plants Baccopa monnieri and Centella asiatica

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    Harsahay Meena

    2012-01-01

    Full Text Available Background: Free radicals or highly reactive oxygen species are capable of inducing oxidative damage to human body. Antioxidants are the compounds which terminate the attack of reactive species and reduce the risk of diseases. Both Baccopa monnieri and Centella asiatica are used in treatment of brain disorders in humans and have almost similar effects. Objective: The study was conducted to determine the antioxidant properties of two well-known memory enhancer medicinal plants Baccopa monnieri and Centella asiatica. Results: The antioxidant activity of these two medicinal plants was evaluated by measuring reducing ability, free radical scavenging activity by DPPH and hydrogen peroxide methods. The antioxidants compounds like ascorbic acid, total phenols and tannins were also evaluated in these plants. Baccopa monnieri and Centella asiatica exhibited significant differences (P<0.05 in their antioxidant values. The methanolic extract of whole leaf powder of Baccopa monnieri exhibited significantly higher antioxidant activity than the Centella asiatica. The antioxidant components viz. ascorbic acid, total phenols and tannins were also found in a higher concentration in Baccopa monnieri as compared to Centella asiatica. Conclusion: It can be concluded from the study that regular use of Baccopa monnieri as a supplement could be more helpful compared to Centella asiatica in treatment of neurological disorders caused by free radical damage.

  5. Antioxidant potential of spices and their active constituents.

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    Srinivasan, K

    2014-01-01

    Excessive free radical generation overbalancing the rate of their removal leads to oxidative stress. Oxidative stress has been implicated in the etiology of cardiovascular disease, inflammatory diseases, cancer, and other chronic diseases. Antioxidants are compounds that hinder the oxidative processes and thereby delay or suppress oxidative stress. There is a growing interest in natural antioxidants found in plants. Herbs and spices are most important targets to search for natural antioxidants from the point of view of safety. A wide variety of phenolic compounds present in spices that are extensively used as food adjuncts possess potent antioxidant, anti-inflammatory, antimutagenic, and cancer preventive activities. This paper reviews a host of spice compounds as exogenous antioxidants that are experimentally evidenced to control cellular oxidative stress, both in vitro and in vivo, and their beneficial role in preventing or ameliorating oxidative-stress-mediated diseases, from atherosclerosis to diabetes to cataract to cancer. The antioxidative effects of turmeric/curcumin, clove/eugenol, red pepper/capsaicin, black pepper/piperine, ginger/gingerol, garlic, onion, and fenugreek, which have been extensively studied and evidenced as potential antioxidants, are specifically reviewed in this treatise.

  6. The antioxidant activity of propofol in chicks

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    A.S. Naser

    2015-06-01

    Full Text Available The aim of this study was to detect the antioxidant effects of propofol in chicks by estimation of glutathione concentration in blood plasma, brain and liver as well as total antioxidant capacity and antioxidant effects of propofol in vitro by using hydrogen peroxide as oxidative stress. Propofol at 20 mg/kg, intraperitoneally significantly increased after 4 hours the concentration of glutathione concentration in plasma and brain compared with the control group and with 5 and 10mg propofol groups. Propofol at 5, 10 and 20 mg/kg, i.p significantly increased glutathione concentration in the liver compared with the control group. Propofol at 5, 10 and 20 mg/kg, i.p increased the efflux rate constant by 882, 1031 and 920 %, increased glutathione turnover rate by 880, 1028, and 917 % and decreased the turnover time by 89, 91 and 90% in the liver. In the brain propofol at 5, 10 and 20 mg/kg, i.p increased efflux rate constant as 26, 600 and 2826 % and increased glutathione turnover rate by 29, 616 and 2894 % and a decreased in the turnover time by 21, 86 and 96%. propofol at 10 and 20 mg/kg, i.p significantly increased after 20 hours the TAC in the serum of the chick by 38 and 48%, respectively compared with the control group. Propofol at concentrations of 25, 50 and 100 micromoles / liter decreased erythrocyte hemolysis induced by hydrogen peroxide in vitro 10 micromoles / liter in a concentration depended manner by 25, 49 and 64 % respectively. In conclusion, propofol have antioxidant effect in vivo and in vitro in the chicks.Propofol have a protection against oxidative stress.

  7. Antioxidant activities of various fruits and vegetables produced in Taiwan.

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    Huang, Hui-Yu; Chang, Chen-Kang; Tso, Tim K; Huang, Ju-Jen; Chang, Wei-Wei; Tsai, Ying-Chieh

    2004-08-01

    Fruits and vegetables have been known to contain a variety of antioxidant components. It has been suggested that antioxidants may protect biomolecules from oxidative damage and therefore be associated with reduced risks of cardiovascular disease and certain cancer. The antioxidant abilities of various parts of eight common fruits and vegetables produced in Taiwan were investigated, including tomato, guava, squash, tangerine, wax gourd, pineapple, chayote, and eggplant. Squash, wax gourd, tomato, and guava seeds showed the highest antioxidant activities in thiobarbituric acid assay. Wax guard and squash seeds showed the highest antioxidant activities in iodometric assay. At the level of 1 g fresh sample, low-density lipoprotein peroxidation was inhibited by at least 90% by tomato meat, guava meat, squash seed, wax gourd meat, core, and seed, and eggplant skin. The total phenolic content was significantly correlated with antioxidant activities measured by thiobarbituric acid (r=0.715, P<0.01) and iodometric (r=0.749, P<0.01) assays. The results of this study could be used for development of merchandise with potential health benefits from agricultural products.

  8. In vitro antimicrobial activity on clinical microbial strains and antioxidant properties of Artemisia parviflora

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    Ahameethunisa Abdul R

    2012-11-01

    Full Text Available Abstract Background Artemisia parviflora leaf extracts were evaluated for potential antimicrobial and antioxidant properties. Antimicrobial susceptibility assay was performed against ten standard reference bacterial strains. Antioxidant activity was analyzed using the ferric thiocyanate and 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH assays. Radical scavenging activity and total phenolic content were compared. Phytochemical analyses were performed to identify the major bioactive constitution of the plant extract. Results Hexane, methanol and ethyl acetate extracts of A. parviflora leaves exhibited good activity against the microorganisms tested. The n-hexane extract of A. parviflora showed high inhibition of the growth of Pseudomonas aeruginosa, Escherichia coli and Shigella flexneri. Methanol extract showed strong radical scavenging and antioxidant activity, other extracts showed moderate antioxidant activity. The major derivatives present in the extracts are of terpenes, steroids, phenols, flavonoids, tannins and volatile oil. Conclusions The results obtained with n-hexane extract were particularly significant as it strongly inhibited the growth of P. aeruginosa, E. coli and S. flexneri. The major constituent of the n-hexane extract was identified as terpenes. Strong antioxidant activity could be observed with all the individual extracts. The antimicrobial and antioxidant property of the extracts were attributed to the secondary metabolites, terpenes and phenolic compounds present in A. parviflora and could be of considerable interest in the development of new drugs.

  9. Canthium parviflorum Leaves: Antioxidant Activity in Food and Biological Systems, pH, and Temperature Stability

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    Vanitha Reddy Palvai

    2014-01-01

    Full Text Available Canthium parviflorum leaves were analyzed for their proximate and phytochemical composition. The leaves were extracted with methanol (ME and analyzed for antioxidant activity by radical scavenging method, reducing power, ferric reducing capacity, and in vitro inhibition of Fenton’s reagent induced oxidation in oil emulsion and microsomes. In addition, the effect of high temperature (100°C, 15 and 30 min and pH (4.5, 7, and 9 on the antioxidant activity of ME was investigated. The leaves were rich in polyphenols, flavonoids β-carotene, glutathione, α-tocopherol, and ascorbic acid. The ME exhibited varying degree of antioxidant activity in a dose dependent manner. The RSA was 68%–500 μg. Reducing potency was 0.34 and FRAP was 1.377. Canthium exhibited greater inhibition of oxidation in microsomes (73% than in the oil emulsion (21%. Heat treatment resulted in reduction of radical scavenging activity of extract from 68% to 40%. At pH 4.5 and 7 methanol extract exhibited some percent of antioxidant activity which ranged between 18 and 32%. Data indicates Canthium as a good source of antioxidants and methanol extract exhibited good antioxidant activity.

  10. Antioxidative activity of ethanol extracts from Spirulina platensis and Nostoc linckia measured by various methods

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    Liliana CEPOI

    2009-11-01

    Full Text Available The goal of this work is to determine the level of antioxidative activity of various ethanol extracts from Spirulina platensis and Nostoc linckia biomass, and also to demonstrate the possibility to select suitable methods for evaluation of these characteristics. The methods for determination of antioxidative activity were selected concerning their possible use for complex preparations: phosphomolybdenum method for evaluation of antioxidant capacity (PMRC, radical-scavenging activity by DPPH method (DPPH, antioxidant activity by the ABTS+ radical cation assay (ABTS, Folin-Ciocalteu reducing capacity (FCRC. We showed the presence of antioxidative substances in ethanol extractions from 2 species of cyanobacteria, and possibility to increase their activity varying ethanol concentration. It facilitates the extraction both water- and lipid-soluble components from biomass. Regarding used methods for antioxidative activity determination, we have used only those based on reaction of electrons return (which widely used nowadays in vitro. Obtained in different ways results demonstrate high reduction capacity of the extracts and possibility to select suitable analytical methods for each case.

  11. The staying power of adhesion-associated antioxidant activity in Mytilus californianus.

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    Miller, Dusty R; Spahn, Jamie E; Waite, J Herbert

    2015-10-06

    The California mussel, Mytilus californianus, adheres in the highly oxidizing intertidal zone with a fibrous holdfast called the byssus using 3, 4-dihydroxyphenyl-l-alanine (DOPA)-containing adhesive proteins. DOPA is susceptible to oxidation in seawater and, upon oxidation, loses adhesion. Successful mussel adhesion thus depends critically on controlling oxidation and reduction. To explore how mussels regulate redox during their functional adhesive lifetime, we tracked extractable protein concentration, DOPA content and antioxidant activity in byssal plaques over time. In seawater, DOPA content and antioxidant activity in the byssus persisted much longer than expected-50% of extractable DOPA and 30% of extractable antioxidant activity remained after 20 days. Antioxidant activity was located at the plaque-substrate interface, demonstrating that antioxidant activity keeps DOPA reduced for durable and dynamic adhesion. We also correlated antioxidant activity to cysteine and DOPA side chains of mussel foot proteins (mfps), suggesting that mussels use both cysteine and DOPA redox reservoirs for controlling interfacial chemistry. These data are discussed in the context of the biomaterial structure and properties of the marine mussel byssus.

  12. Synthesis, antimicrobial and antioxidative activity of some new isatin derivatives

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    Šekularac Gavrilo M.

    2014-01-01

    Full Text Available The isatin derivatives, Schiff bases, were synthesized by the reaction of isatin and various substituted primary amines and characterized by several spectroscopic methods. Investigation of the antimicrobial activity of the synthesized compounds was performed by the agar dilution method, against different strains of bacteria and one fungi. The antioxidative activity of the synthesized compounds was also determined. Some of the compounds have shown the significant activity against the selected strains of microorganisms and the antioxidative activity. [Projekat Ministarstva nauke Republike Srbije, br. 172013 i III 46010

  13. Hypolipidemic, Antioxidant and Antiinflammatory Activities of Microalgae Spirulina

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    Deng, Ruitang; Chow, Te-Jin

    2010-01-01

    Spirulina is free-floating filamentous microalgae growing in alkaline water bodies. With its high nutritional value, Spirulina has been consumed as food for centuries in Central Africa. It is now widely used as nutraceutical food supplement worldwide. Recently, great attention and extensive studies have been devoted to evaluate its therapeutic benefits on an array of diseased conditions including hypercholesterolemia, hyperglycerolemia, cardiovascular diseases, inflammatory diseases, cancer and viral infections. The cardiovascular benefits of Spirulina are primarily resulted from its hypolipidemic, antioxidant and antiinflammatory activities. Data from preclinical studies with various animal models consistently demonstrate the hypolipidemic activity of Spirulina. Although differences in study design, sample size and patient conditions resulting in minor inconsistency in response to Spirulina supplementation, the findings from human clinical trials are largely consistent with the hypolipidemic effects of Spirulina observed in the preclinical studies. However, most of the human clinical trials are suffered with limited sample size and some with poor experimental design. The antioxidant and/or antiinflammatory activities of Spirulina were demonstrated in a large number of preclinical studies. However, a limited number of clinical trials have been carried out so far to confirm such activities in human. Currently, our understanding on the underlying mechanisms for Spirulina’s activities, especially the hypolipidemic effect, is limited. Spirulina is generally considered safe for human consumption supported by its long history of use as food source and its favorable safety profile in animal studies. However, rare cases of side-effects in human have been reported. Quality control in the growth and process of Spirulina to avoid contamination is mandatory to guarantee the safety of Spirulina products. PMID:20633020

  14. Phytochemical analysis and in vitro antioxidant activity of Uncaria gambir

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    Mohd Amir

    2012-01-01

    Full Text Available The present research work was carried out to evaluate the antioxidant potential and antiradical property of methanolic extract of Uncaria gambir. Antioxidant and radical scavenging activity were determined by using different in vitro assays including reducing power assay, superoxide anion scavenging activity assay, hydroxyl radical scavenging activity assay, nitric oxide scavenging activity assay, DPPH free radical scavenging assay and hydrogen peroxide method. Preliminary phytochemical screening revealed that the extract of U. gambir possesses flavonoids, alkaloids and phenolic materials. In the present investigation, quantitative estimation of flavonoids content and phenolic content was also carried out by colorimetric methods, using aluminium chloride and Folin-Ciocalteu reagent method, respectively, to establish relationship between antioxidant activity and total phenolics and flavonoid contents. The total phenolic and flavonoid contents were found to be 18.37±2.79 mg gallic acid equivalents (GAE/g dry weight and 5.82±2.23 mg rutin equivalents (RE/g dry weight, respectively. The extract showed significant antioxidant activity in a dose-dependent manner in all the assays. The IC 50 values of all parameters were determined. Ascorbic acid was used as standard. The results obtained in the present study indicated that U. gambir extract could be a potential source of natural antioxidant.

  15. Antidiabetic and antioxidant activities of Nypa fruticans Wurmb. vinegar sample from Malaysia.

    Science.gov (United States)

    Yusoff, Nor Adlin; Yam, Mun Fei; Beh, Hooi Kheng; Abdul Razak, Khairul Niza; Widyawati, Tri; Mahmud, Roziahanim; Ahmad, Mariam; Asmawi, Mohd Zaini

    2015-08-01

    To study the antidiabetic and antioxidant activities of nipa palm vinegar (NPV) used in traditional Malay medicine for treating diabetes. NPV was extracted using liquid-liquid extraction method and the obtained samples were subjected to antidiabetic studies using normal and streptozotocin-induced diabetic rat models whereas antidoxidant activities were investigated via in vitro antioxidant tests namely 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid free radicals scavenging activities and the reducing power assay. Single administration of NPV and its extracts were not effective in both normal and diabetic rats. In intraperitoneal glucose tolerance test, NPV and its aqueous extract showed significant blood glucose lowering effect. In the sub-acute study, compared with the diabetic control, aqueous extract of NPV showed the most notable blood glucose lowering effect (56.6%) and a significant improvement in serum insulin levels (79.8%, P < 0.05). To assess NPV's antioxidant activity, three in vitro antioxidant tests were employed: 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis-3-ethylbenzothiozoline-6-sulfonic acid free radical-scavenging assays, and the reducing power assay. Ethyl acetate extract had the greatest antioxidant potential and content of phenolic and flavonoid compounds. A linear positive correlation between the antioxidant parameters was observed. Chemical profiling analysis of aqueous extract of NPV revealed the presence of acetic acid (35.25%), the main active constituent which significantly contributed to the observed antidiabetic activity. Aqueous extract of NPV possesses antihyperglycaemic activities comparable to the metformin, while the ethyl acetate extract precipitated significant antioxidant effects attributable to its high phenolic content. These findings suggest that antioxidant compounds of NPV do not contribute much towards the overall observed antidiabetic effect. Copyright © 2015 Hainan Medical

  16. Improved green coffee oil antioxidant activity for cosmetical purpose by spray drying microencapsulation

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    Anna B.F.L. Nosari

    2015-06-01

    Full Text Available AbstractThe oil extracted by cold pressing unroasted coffee beans, known as green coffee oil, has been widely used for cosmetic purposes. The objective of this work was to prepare and characterize microcapsules containing green coffee oil and to verify its antioxidant activity under the effect of light, heat and oxygen. The encapsulating material was arabic gum and the microcapsules were obtained by spray drying an oil-in-water emulsion containing green coffee oil. The characterization of the microcapsules was performed by laser diffraction, scanning electron microscopy, differential scanning calorimetry and the antioxidant activity. The antioxidant activity was determined by a modified active oxygen method with light irradiation, heating and oxygen flux. The microparticles were effectively produced by the proposed spray drying method, which resulted in green coffee oil loads of 10 and 30%. The morphological evaluation of microcapsules showed spherical shape with smooth and non-porous surfaces, demonstrating the adequacy of arabic gum as encapsulating material. Calorimetric analysis of individual components and microcapsules with 10 and 30% green coffee oil showed diminished degradation temperatures and enthalpy, suggesting a possible interaction between arabic gum and green coffee oil. The antioxidant activities for pure green coffee oil and its microcapsules with loads of 10 and 30% showed high activity when compared to the reference antioxidant alfa-tocopherol. Microcapsules containing 10 and 30% of oil showed 7-fold and 3-fold increase in antioxidant activity when compared to pure green coffee oil. The new method for antioxidant activity determination proposed here, which applies heat, light and oxygen simultaneously, suggests a high improvement in encapsulated green coffee oil when compared to this active alone. The results showed herein indicate a promising industrial application of this microencapsulated green coffee oil.

  17. Antioxidant activity and protecting health effects of common medicinal plants.

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    Škrovánková, Soňa; Mišurcová, Ladislava; Machů, Ludmila

    2012-01-01

    Medicinal plants are traditionally used in folk medicine as natural healing remedies with therapeutic effects such as prevention of cardiovascular diseases, inflammation disorders, or reducing the risk of cancer. In addition, pharmacological industry utilizes medicinal plants due to the presence of active chemical substances as agents for drug synthesis. They are valuable also for food and cosmetic industry as additives, due to their preservative effects because of the presence of antioxidants and antimicrobial constituents. To commonly used medicinal plants with antioxidant activity known worldwide belong plants from several families, especially Lamiaceae (rosemary, sage, oregano, marjoram, basil, thyme, mints, balm), Apiaceae (cumin, fennel, caraway), and Zingiberaceae (turmeric, ginger). The antioxidant properties of medicinal plants depend on the plant, its variety, environmental conditions, climatic and seasonal variations, geographical regions of growth, degree of ripeness, growing practices, and many other factors such as postharvest treatment and processing. In addition, composition and concentration of present antioxidants, such as phenolic compounds, are related to antioxidant effect. For appropriate determination of antioxidant capacity, the extraction technique, its conditions, solvent used, and particular assay methodology are important.

  18. [ANTIOXIDANT DYSFUNCTIONALITY OF HIGH-DENSITY LIPOPROTEINS (HDL) IN DECOMPENSATED DIABETIC PATIENTS].

    Science.gov (United States)

    Awad, Fernanda; Contreras-Duarte, Susana; Molina, Patricia; Quiñones, Verónica; Serrano, Valentina; Abbott, Eduardo; Maiz, Alberto; Busso, Dolores; Rigotti, Attilio

    2015-09-01

    high density lipoproteins (HDL) have important cardiovascular protective effects mediated by their role in reverse cholesterol transport as well as other functional activities, including significant anti-inflammatory and antioxidant properties. It has been shown that HDL anti-inflammatory and antioxidant functions are defective in metabolically stable diabetic patients; however they have not been evaluated during a hyperglycemic crisis. to determine the antioxidant activity of HDL during a severe diabetic decompensation and to analyze whether this function is restored after resolution of the acute event. the antioxidant activity of HDL was measured in vitro by a fluorescent assay in plasma samples obtained from diabetic patients with acute metabolic decompensation at admission, recovery within the hospital and follow-up in ambulatory care. As a comparison, HDL particles from some healthy subjects were used as controls. the HDL antioxidant function was significantly reduced in patients during an acute diabetic decompensation compared with the control group, and was gradually restored reaching normal values during the ambulatory follow-up. Hyperglycemic crisis also showed low plasma paraoxonase-1 activity, which increased significantly during at follow-up. HDL particles isolated from acute diabetic descompensated patients exhibit a significantly and reversibly low antioxidant capacity, which is probably due to a reduced paraoxonase-1 activity. Copyright AULA MEDICA EDICIONES 2014. Published by AULA MEDICA. All rights reserved.

  19. Anti-oxidation activity of ethanol extracts from natural thalli of lichens

    Institute of Scientific and Technical Information of China (English)

    Kojiro HARA; Marie ENDO; Hiroko KAWAKAMI; Masashi KOMINE; Yoshikazu YAMAMOTO

    2011-01-01

    Screening test on anti-oxidation activity using 1,1-diphenyl2-picrylhydrazyl(DPPH) was performed for 99 ethanol extracts of 85 species of natural thalli of lichens in order to find novel anti-oxidation compounds.The 17 extracts of natural thalli showed high anti-oxidation activity.Among them,the activities of extracts from Hypogymnia vittata,Peltigera aphthosa,Nephromopsis ornata,Pseudevernia furfuracea,Cladonia vulcani and Peltigera elizabethae were higher.Extracts of Peltigera spp.showed higher activity than those of other genera.The ethanol extract of P.aphthosa had been separated into ethyl acetate-soluble and water-soluble fractions.Two anti-oxidative spots were found only in the water-soluble fractions by thin-layer chromatography.The compound in the lower spot had the same Rf value,UV spectrum,and color as authentic solorinine that was previously found as a unique quaternary ammonium compound from Peltigera spp.We now report that the hydrophilic lichen substance,solorinine showed a nearly same anti-oxidation activity (EC50=120μmol/Lol/L) as standard antioxidant Trolox (EC50=150μmol/L).

  20. In vitro and in vivo antioxidant activity of ethanolic extract of white button mushroom (Agaricus bisporus).

    Science.gov (United States)

    Liu, Jun; Jia, Liang; Kan, Juan; Jin, Chang-Hai

    2013-01-01

    The antioxidant activities of ethanolic extract from edible mushroom Agaricus bisporus (A. bisporus) were evaluated by various methods in vitro and in vivo. In antioxidant assays in vitro, ethanolic extract of A. bisporus was found to have strong reducing power, superoxide radical, hydroxyl radical and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, and moderate hydrogen peroxide scavenging activity. In antioxidant assays in vivo, mice were administered with ethanolic extract of A. bisporus via gavage for 30 consecutive days. As a result, administration of ethanolic extract significantly enhanced the activities of antioxidant enzymes in serums, livers and hearts of mice. In addition, the total phenolic content in the extract determined by Folin-Ciocalteu method was 6.18mg of gallic acid equivalents per gram of dry weight. The main phenolic compounds in ethanolic extract analyzed by ultra-high performance liquid chromatography tandem mass spectrometry were determined as gallic acid, protocatechuic acid, catechin, caffeic acid, ferulic acid and myricetin. These results suggested that ethanolic extract of A. bisporus had potent antioxidant activity and could be explored as a novel natural antioxidant.

  1. Correlation between Antioxidant Activities and Phenolic Contents of Radix Angelicae Sinensis (Danggui

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    Ling Huang

    2009-12-01

    Full Text Available Radix Angelicae Sinensisis (RAS is one of the most popular traditional Chinese herbal medicines. In the present study, six RAS extracts (i.e., phenolic extract PE, petroleum ether extract PEE, ethyl acetate extract EAE, absolute ethanol extract AEE, 95% ethanol extract 95 EE, and water extract WE were prepared and their antioxidant activities measured by DPPH (1,1-diphenyl-2-picrylhydrazyl radical, ABTS [2,2′-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid diammonium salt], Reducing power, •O2– and lipid peroxidation assays. In general, PE, PEE and EAE had relatively high antioxidant activity, followed by AEE with moderate activity, as compared with 95 EE and WE that had low activity. Their phenolic contents (including total phenolic, ferulic acid, caffeic acid, same as below were then determined by HPLC or spectrophotometry. The sequence of phenolic contents was roughly identical with that of antioxidant activity. When the values of 1/IC50 of various antioxidant assays were used to evaluate the level of antioxidant of the RAS extracts, (plot between 1/IC50 values and phenolic contents, the correlation coefficient (R ranged from 0.642 to 0.941, with an average value of 0.839. Significant positive correlations demonstrated that the antioxidant effects of RAS might generally be considered a result of the presence of the phenolic compounds, especially ferulic acid and caffeic acid.

  2. Spectroscopic studies on the antioxidant activity of ellagic acid

    Science.gov (United States)

    Kilic, Ismail; Yeşiloğlu, Yeşim; Bayrak, Yüksel

    2014-09-01

    Ellagic acid (EA, C14H6O8) is a natural dietary polyphenol whose benefits in a variety of diseases shown in epidemiological and experimental studies involve anti-inflammation, anti-proliferation, anti-angiogenesis, anticarcinogenesis and anti-oxidation properties. In vitro radical scavenging and antioxidant capacity of EA were clarified using different analytical methodologies such as total antioxidant activity determination by ferric thiocyanate, hydrogen peroxide scavenging, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, 2,2‧-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and superoxide anion radical scavenging, ferrous ions (Fe2+) chelating activity and ferric ions (Fe3+) reducing ability. EA inhibited 71.2% lipid peroxidation of a linoleic acid emulsion at 45 μg/mL concentration. On the other hand, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), α-tocopherol and ascorbic acid displayed 69.8%, 66.8%, 64.5% and 59.7% inhibition on the peroxidation of linoleic acid emulsion at the same concentration, respectively. In addition, EA had an effective DPPH• scavenging, ABTSrad + scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, ferric ions (Fe3+) reducing power and ferrous ions (Fe2+) chelating activities. Also, those various antioxidant activities were compared to BHA, BHT, α-tocopherol and ascorbic acid as references antioxidant compounds. These results suggested that EA can be used in the pharmacological, food industry and medicine because of these properties.

  3. The study of antioxidant activities of fucoidan from Laminaria japonica

    Institute of Scientific and Technical Information of China (English)

    Zhao Xue; Xue Changhu; Cai Yuepiao; Wang Dongfeng; Fang Yu

    2005-01-01

    Two large molecular weight fucoidan fractions F-A and F-B were obtained by water extraction and anion-exchange chromatography and then L-A and L-B with low molecular weight and uronic acid content were prepared by radical process degradation of F-A and F-B. The antioxidant activities of the fucoidan fractions on two oxygen free radicals and Cu2+-induced low-density lipoprotein (LDL) oxidation were studied. The results show that all the fucoidan fractions had excellent scavenging capacities on superoxide radical and hypochlorous acid, except the highly sulfated fraction L-B. In LDL oxidation system, low molecular weight fractions L-A and L-B exhibited great inhibitory effects on LDL oxidation induced by Cu2+, however F-A and F-B had little inhibitory effects in this system due to their large molecular weights. The present results suggest that fucoidan from Laminaria japonica is an excellent natural antioxidant and has great potential for preventing the free radical-mediated diseases.

  4. In Vivo Antioxidant Activity of Deacetylasperulosidic Acid in Noni

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    De-Lu Ma

    2013-01-01

    Full Text Available Deacetylasperulosidic acid (DAA is a major phytochemical constituent of Morinda citrifolia (noni fruit. Noni juice has demonstrated antioxidant activity in vivo and in human trials. To evaluate the role of DAA in this antioxidant activity, Wistar rats were fed 0 (control group, 15, 30, or 60 mg/kg body weight per day for 7 days. Afterwards, serum malondialdehyde concentration and superoxide dismutase and glutathione peroxidase activities were measured and compared among groups. A dose-dependent reduction in malondialdehyde was evident as well as a dose-dependent increase in superoxide dismutase activity. DAA ingestion did not influence serum glutathione peroxidase activity. These results suggest that DAA contributes to the antioxidant activity of noni juice by increasing superoxide dismutase activity. The fact that malondialdehyde concentrations declined with increased DAA dose, despite the lack of glutathione peroxidase-inducing activity, suggests that DAA may also increase catalase activity. It has been previously reported that noni juice increases catalase activity in vivo but additional research is required to confirm the effect of DAA on catalase. Even so, the current findings do explain a possible mechanism of action for the antioxidant properties of noni juice that have been observed in human clinical trials.

  5. Antioxidant and cytotoxic activity of Acanthus ilicifolius flower

    Institute of Scientific and Technical Information of China (English)

    Muhamad Firdaus; Asep Awaludin Prihanto; Rahmi Nurdiani

    2013-01-01

    Objective: To investigate the antioxidant and cytotoxic activity of the flower of Acanthus ilicifolius (A. ilicifolius). Methods: Antioxidant activity was determined as antiradical efficiency with diphenyl picrylhydrazil (DPPH) method and cytotoxic assay was undertaken using brine shrimp lethal toxicity test. Results: A. ilicifolius flower contained terpenoid, phenolic compounds, and alkaloid. The methanol extract of A. ilicifolius flower showed the highest antiradical efficiency (AE=1.41í10-3) against DPPH radicals and the highest cytotoxicity (LC50=22 μg/mL) against brine shrimp nauplii. Conclusions: It is suggested that active compounds of A. ilicifolius flower solved in methanol play a role to inhibit free radical activity and kill Artemia salina nauplii. The substances can be considered as potential antioxidant and cytotoxic agents as well as imminent candidate for cancer therapy.

  6. Antioxidant and cytotoxic activity of Acanthus ilicifolius flower

    Institute of Scientific and Technical Information of China (English)

    Muhamad; Firdaus; Asep; Awaludin; Prihanto; Rahmi; Nurdiani

    2013-01-01

    Objective:To investigate the antioxidant and cytotoxic activity of the flower of Acanthus ilicifolius(A.ilicifolius).Methods:Antioxidant activity was determined as antiradical efficiency with diphenyl picrylhydrazil(DPPH)method and cytotoxic assay was undertaken using brine shrimp lethal toxicity test.Results:A.ilicifolius flower contained terpenoid,phenolic compounds,and alkaloid.The methanol extract of A.ilicifolius flower showed the highest antiradical efficiency(AE=1.41×10-3)against DPPH radicals and the highest cytotoxicity(LC50=22μg/mL)against brine shrimp nauplii.Conclusions:It is suggested that active compounds of A.ilicifolius flower solved in methanol play a role to inhibit free radical activity and kill Artemia salina nauplii.The substances can be considered as potential antioxidant and cytotoxic agents as well as imminent candidate for cancer therapy.

  7. Antioxidant activity of twenty five plants from Colombian biodiversity.

    Science.gov (United States)

    Mosquera, Oscar M; Correa, Yaned M; Buitrago, Diana C; Niño, Jaime

    2007-08-01

    The antioxidant activity of the crude n-hexane, dichloromethane, and methanol extracts from 25 species belonging to the Asteraceae, Euphorbiaceae, Rubiaceae, and Solanaceae families collected at natural reserves from the Eje Cafetero Ecorregión Colombia, were evaluated by using the spectrophotometric 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging method. The strongest antioxidant activities were showed by the methanol and dichloromethane extracts from the Euphorbiaceae, Alchornea coelophylla (IC50 41.14 mg/l) and Acalypha platyphilla (IC50 111.99 mg/l), respectively. These two species had stronger DPPH radical scavenging activities than hydroquinone (IC50 151.19 mg/l), the positive control. The potential use of Colombian flora for their antioxidant activities is discussed.

  8. The antioxidant and cytotoxic activities of Sonchus oleraceus L. extracts

    OpenAIRE

    Yin, Jie; Kwon, Gu-Joong; Wang, Myeong-Hyeon

    2007-01-01

    This study investigated in vitro antioxidant activity of Sonchus oleraceus L. by extraction solvent, which were examined by reducing power, hydroxyl radical-scavenging activity(HRSA) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays. 70% MeOH extract had the greatest reducing power while EtOH extract had the greatest HRSA. The antioxidant activity of S. oleraceus extracts was concentration dependent and its IC50 values ranged from 47.1 to 210.5 µg/ml and IC50 of 70% MeOH, bo...

  9. Botanical origin causes changes in nutritional profile and antioxidant activity of fermented products obtained from honey.

    Science.gov (United States)

    Dezmirean, Graţia I; Mărghitaş, Liviu A; Bobiş, Otilia; Dezmirean, Daniel S; Bonta, Victoriţa; Erler, Silvio

    2012-08-15

    Honey as rich source of enzymatic and nonenzymatic antioxidants serves as health-promoting nutrient in the human body. Here, we present the first time a comparative study of nutritional profiles (e.g., acidities, sugar, organic acid profile, total polyphenolic, flavonoid content) for different unifloral, multifloral honeys and their fermented products, in correlation with their antioxidant activity. Additionally, an optimized method for HPLC separation of organic acids from honey was established. The total phenolic content of honey samples varied widely among the honey types compared to fermented products. High amounts of total flavonoids were quantified in heather honey, followed by raspberry, multifloral, black locust, and linden honey. A positive correlation between the content of polyphenols, flavonoids, and antioxidant activity was observed in honey samples. After fermentation, the flavonoid content of dark honey fermented products decreased significantly. Black locust and linden honeys are more suitable for fermentation because the decrease in antioxidant substances is less pronounced.

  10. Effect of chlorogenic acid on antioxidant activity of Flos Lonicerae extracts

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Flos Lonicerae is a medically useful traditional Chinese medicine herb. However, little is known about the antioxidant properties ofFlos Lonicerae extracts. Here the antioxidant capacity of water, methanolic and ethanolic extracts prepared from Flos Lonicerae to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and reduce Fe3+ to Fe2+ is examined. Chlorogenic acid, a major component of Flos Lonicerae, is identified and further purified from 70% ethanolic extract with high performance liquid chromatography (HPLC) and its antioxidant capacity is characterized. The total phenolic compounds and chlorogenic acid contents in Flos Lonicerae are determined. The present results demonstrate that the Flos Lonicerae extracts exhibit antioxidant activity and chlorogenic acid is a major contributor to this activity.

  11. Sulfation, anticoagulant and antioxidant activities of polysaccharide from green algae Enteromorpha linza.

    Science.gov (United States)

    Wang, Xiaomei; Zhang, Zhongshan; Yao, Zhiyun; Zhao, Mingxing; Qi, Huimin

    2013-07-01

    Sulfated polysaccharides exerted potent biological property which was relative to degree of sulfation, molecular weight, substitution position and chain conformation. In present study, the polysaccharide with low molecular weight (LEP) from Enteromorpha linza was sulfated with chlorosulfuric acid in formamide. The obtained polysaccharide sulfate was selected to evaluate their antioxidant activities and the anticoagulant activity in the coagulation assays, activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT). The data obtained in vitro models indicated that high DS and moderate Mw showed the best anticoagulant and antioxidant activities.

  12. Antioxidant activity of ultrasonic extracts of leek Allium porrum L.

    Directory of Open Access Journals (Sweden)

    Mladenović Jelena D.

    2011-01-01

    Full Text Available This study was aimed at evaluating the antioxidant activity and efficacy of the ethanolic extract of the ultrasonic extracts of leek Allium porrum L. Ethanolic (50%; v/v extracts of edible leek parts (stem and leaf were prepared by ultrasound-assisted extraction, which was followed by evaluation of total phenols, flavonoids and antioxidant activity. Total phenols were determined using the modified Folin-Ciocalteu method. Antioxidant activity was assessed by scavenging the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH. The results of antioxidant activity were compared with control antioxidants: vitamin C and BHT. The higher content of phenols (69.46 mg GAE/g dry extract and flavonoids (33.53 mg CE/g dry extract was found in the ethanolic extract of leek stem. The measured values of IC50 were 98.90 g/ml and 61.05 g/ml for the ethanolic extract of leek leaf and stem, respectively.

  13. Comparison of phytochemical profiles, antioxidant and cellular antioxidant activities of different varieties of blueberry (Vaccinium spp.).

    Science.gov (United States)

    Wang, Huailing; Guo, Xinbo; Hu, Xiaodan; Li, Tong; Fu, Xiong; Liu, Rui Hai

    2017-02-15

    Numerous reports have demonstrated that the consumption of fruits and vegetables is beneficial for the human health. Blueberries, in particular, are rich in phytochemicals including free and bound forming. Phytochemical profiles of 14 varieties of blueberry were compared in this study. 12 compounds were analyzed and had significant changes in blueberry fruits. Total antioxidant activities in different blueberry varieties varied about 2.6times by oxygen radical absorbance capacity (ORAC) assay, and 2times by peroxyl radical scavenging capacity (PSC) assay. The cellular antioxidant activities (CAA) in different varieties varied about 3.9times without phosphate buffer saline (PBS) wash, and 4.7times with PBS wash by CAA assay. Blueberry extracts had potent antiproliferative activities against HepG2 human liver cancer cells, indicating the potential protective benefits associated with their use as functional foods. The anti-proliferative activity was observed to be dose-dependent in blueberry extracts.

  14. Antioxidant and cytotoxic activity of Acanthus ilicifolius flower

    Directory of Open Access Journals (Sweden)

    Muhamad Firdaus

    2013-01-01

    Conclusions: It is suggested that active compounds of A. ilicifolius flower solved in methanol play a role to inhibit free radical activity and kill Artemia salina nauplii. The substances can be considered as potential antioxidant and cytotoxic agents as well as imminent candidate for cancer therapy.

  15. In vitro Antifungal, Antioxidant and Cytotoxic Activities of a Partially ...

    African Journals Online (AJOL)

    greater in vitro antioxidant activity than the aqueous extract. SDS-PAGE .... was added to 4 ml of 0.004 % methanol solution of DPPH. After a ... Superoxide anion scavenging activity ... SDS-PAGE was performed to identify the proteins in ...

  16. Evaluation of antioxidant, enzyme inhibition, and cytotoxic activity of three anthraquinones (alizarin, purpurin, and quinizarin).

    Science.gov (United States)

    Zengin, G; Degirmenci, N S; Alpsoy, L; Aktumsek, A

    2016-05-01

    The aim of this work was to investigate the cytotoxic, antioxidative, and enzyme inhibition effects of alizarin, quinizarin, and purpurin, which are anthraquinones (AQ). Cytotoxic effects were evaluated with cell inhibition rate by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide assay. Different chemical assays, including free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzothiazloine-6-sulfonic acid)), phosphomolybdenum and reducing power (ferric reducing antioxidant power and cupric ion reducing activity), were used to evaluate the antioxidant properties. Moreover, enzyme inhibitory activities were analyzed against acetylcholinesterase, butrylcholinesterase, tyrosinase, α-amylase, and α-glucosidase. These components have antioxidant and enzyme inhibition activity. Especially, purpurin showed the strongest antioxidant and good enzyme inhibitory effects. According to our cytotoxicity results, alizarin, purpurin, and quinizarin induced dose- and time-dependent cell proliferation. Furthermore, when we applied AQs with mitomycin C (MC) on L929 cell line, we demonstrated that cell proliferation in MC-AQ groups compared with MC group was increased. The most effective component was alizarin at 100 µM concentration. These AQs showed positive effects on L929 cell lines with high half-maximal inhibitory concentration values. Our results demonstrate that AQs may be used as antioxidative compounds in food and medicinal applications. © The Author(s) 2015.

  17. PHENOLIC COMPOUNS AND ANTIOXIDANT ACTIVITY OF MONOVARIETAL RED WINES PRODUCED IN LIMBACH REGION

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    Tomáš Tóth

    2012-02-01

    Full Text Available Chosen antioxidant properties as content of total polyphenols, content of total anthocyanins and antioxidant activity of the red wines, originating from winery region Limbach, were determined. Three types of mono-varietal wines – Cabernet Sauvignon, Blaufränkisch and St. Laurent, originating from 3 local producers were analysed. Total polyphenols content was determined with phenol Folin-Ciocalteau´s reagent, total anthocyanins content was determined by pH differential method and antioxidant activity was determined using DPPH stable free radical. All three parameters were determined by UV-VIS absorption spectrometry. The determined total polyphenol contents in observed wines were within the interval 1908 – 3276 mg gallic acid.l-1, total anthocyanin contents within the interval 96 – 334 mg.l-1 and antioxidant activity within the interval 65.5 – 84.1 %. This result indicates high antioxidant properties of the Limbach´s wines. The differences between total polyphenol contents of the wines originating from different producers were not statistically significant. However, we found significant differences between results of the rest analysed parameters (total anthocyanins content and antioxidant activity of the wines originating from different producers. The differences between results of all analysed properties of the wines of different variety were not statistically significant.

  18. Antioxidant activities of Indigofera cassioides Rottl. Ex. DC. using various in vitro assay models

    Institute of Scientific and Technical Information of China (English)

    R Senthil Kumar; B Rajkapoor; P Perumal

    2012-01-01

    Objective: To evaluate the antioxidant potential of methanolic leaf extract of Indigoferacassioides (MEIC) using various in vitro antioxidant assay systems. Methods: Antioxidant and free radical scavenging activity of MEIC was assayed by using different in vitro models like ABTS, DPPH, nitric oxide, superoxide, hydrogen peroxide and hydroxyl radical. Reductive ability of the extract was tested by the complex formation with potassium ferricyanide. Further total phenol and flavonoid contents of the crude extract were also determined. Rutin and ascorbic acid were used as standards. Results: MEIC exhibited potent and concentration dependent free radical scavenging activity in all the tested models. Reductive ability was also found to increase with increase in MEIC concentration. Total phenol and flavonoid content determination showed that the extract is rich in phenols and flavonoids. Conclusions: All the results of the in vitro antioxidant assays reveal potent antioxidant and free radical scavenging activity of the leaves of Indigofera cassioides, equivalent to that of standard ascorbic acid and rutin. This potent antioxidant activity may be attributed to its high phenolic and flavonoid contents

  19. Antioxidant activity of oligomeric acylphloroglucinols from Myrtus communis L.

    Science.gov (United States)

    Rosa, Antonella; Deiana, Monica; Casu, Viviana; Corona, Giulia; Appendino, Giovanni; Bianchi, Federica; Ballero, Mauro; Dessì, M Assunta

    2003-09-01

    The use of myrtle (Myrtus communis L.) as a culinary spice and as a flavoring agent for alcoholic beverages is widespread in the Mediterranean area, and especially in Sardinia. Myrtle contains unique oligomeric non-prenylated acylphloroglucinols, whose antioxidant activity was investigated in various systems. Both semimyrtucommulone (1) and myrtucommulone A (2) showed powerful antioxidant properties, protecting linoleic acid against free radical attack in simple in vitro systems, inhibiting its autoxidation and its FeCl3- and EDTA-mediated oxidation. While both compounds lacked pro-oxidant activity, semimyrtucommulone was more powerful than myrtucommulone A, and was further evaluated in rat liver homogenates for activity against lipid peroxidation induced by ferric-nitrilotriacetate, and in cell cultures for cytotoxicity and the inhibition of TBH- or FeCl3-induced oxidation. The results of these studies established semimyrtucommulone as a novel dietary antioxidant lead.

  20. Phenolic Composition and Antioxidant Activity of Malus domestica Leaves

    Directory of Open Access Journals (Sweden)

    Mindaugas Liaudanskas

    2014-01-01

    Full Text Available The aim of this study was to determine the composition and content of phenolic compounds in the ethanol extracts of apple leaves and to evaluate the antioxidant activity of these extracts. The total phenolic content was determined spectrophotometrically, as well as the total flavonoid content in the ethanol extracts of apple leaves and the antioxidant activity of these extracts, by the ABTS, DPPH, and FRAP assays. The highest amount of phenolic compounds and flavonoids as well as the highest antioxidant activity was determined in the ethanol extracts obtained from the apple leaves of the cv. Aldas. The analysis by the HPLC method revealed that phloridzin was a predominant component in the ethanol extracts of the apple leaves of all cultivars investigated. The following quercetin glycosides were identified and quantified in the ethanol extracts of apple leaves: hyperoside, isoquercitrin, avicularin, rutin, and quercitrin. Quercitrin was the major compound among quercetin glycosides.

  1. Antioxidant activity of fraction of Tephrosia purpurea linn.

    Directory of Open Access Journals (Sweden)

    Soni K

    2006-01-01

    Full Text Available The ethanol extract of Tephrosia purpurea Linn. (Family: Leguminosae was found to significantly inhibit the carbon tetrachloride-induced lipid peroxidation in vivo and superoxide generation in vivo . The ethyl acetate fraction of the same extract was studied for free radical scavenging and antilipid peroxidation activity. The IC50 values in both of these in vitro assays were found to be significantly reduced for ethyl acetate fraction compared with the ethanolic extract of the plant. The observation was further supported by comparing the in vivo antioxidant activity for both the ethanolic extract and its ethyl acetate fraction. The study concluded that the ethanolic extract of T. purpurea exhibits antioxidant activity in vivo and the ethyl acetate soluble fraction has improved antioxidant potential than the extract.

  2. Use of Different Proteases to Obtain Flaxseed Protein Hydrolysates with Antioxidant Activity.

    Science.gov (United States)

    Karamać, Magdalena; Kosińska-Cagnazzo, Agnieszka; Kulczyk, Anna

    2016-06-29

    The antioxidant activity of flaxseed protein hydrolysates obtained using five different enzymes was evaluated. Proteins were isolated from flaxseed cake and were separately treated with papain, trypsin, pancreatin, Alcalase and Flavourzyme. The degree of hydrolysis (DH) was determined as the percentage of cleaved peptide bonds using a spectrophotometric method with o-phthaldialdehyde. The distribution of the molecular weights (MW) of the hydrolysis products was profiled using Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Tricine-SDS-PAGE) and size exclusion-high performance liquid chromatography (SE-HPLC) separations. The antioxidant activities of the protein isolate and hydrolysates were probed for their radical scavenging activity using 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonate) radical cation (ABTS(•+)) and photochemiluminescence (PCL-ACL) assays, and for their ferric reducing antioxidant power (FRAP) and ability to bind Fe(2+). The hydrolysates were more effective as antioxidants than the protein isolate in all systems. The PCL-ACL values of the hydrolysates ranged from 7.2 to 35.7 μmol Trolox/g. Both the FRAP and ABTS(•+) scavenging activity differed among the hydrolysates to a lower extent, with the ranges of 0.20-0.24 mmol Fe(2+)/g and 0.17-0.22 mmol Trolox/g, respectively. The highest chelating activity (71.5%) was noted for the pancreatin hydrolysate. In general, the hydrolysates obtained using Alcalase and pancreatin had the highest antioxidant activity, even though their DH (15.4% and 29.3%, respectively) and the MW profiles of the peptides varied substantially. The O₂(•-) scavenging activity and the ability to chelate Fe(2+) of the Flavourzyme hydrolysate were lower than those of the Alcalase and pancreatin hydrolysates. Papain was the least effective in releasing the peptides with antioxidant activity. The study showed that the type of enzyme used for flaxseed protein hydrolysis determines the antioxidant activity

  3. Evaluation of Antioxidant Activities of Cereals and their Malts

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    Ondrejovič Miroslav

    2014-12-01

    Full Text Available The aim of this study was to determine the influence of malting on the antioxidant content in cereals such as wheat (PS Sunanka, Zaira, PS 57/11 and Vanda, oat (Dunajec and barley (Laudis 550 harvested in 2013. Antioxidant and polyphenol contents of these cereals and malts were investigated. Secondary, technological parameters of prepared malts were evaluated and compared with malt from barley Laudis 550 used as reference material. Malting of selected cereals had an impact on antioxidant and polyphenol content and allowed a better extraction of these compounds from cereal matrix, except of barley malt, whose antioxidant and total polyphenol content remained comparable. For other cereal malts, antioxidant contents were 2.0, 1.8, 2.6, 2.9 and 3.2-fold higher and total polyphenol content were 1.8, 1.9, 1.9, 3.1 and 3.4-fold higher than in wheat (PS Sunanka, Zaira, PS 57/11, Vanda and oat (Dunajec, respectively. From correlation analysis, the results showed that not all polyphenols released by malting have antioxidant activity. Technological parameters (friability, haze of wort, saccharification rate, filtration rate, extract and diastatic power also indicated that good malt quality had oat Dunajec and wheat PS Sunanka and Zaira in comparison with reference material (barley Laudis 550.

  4. [Antioxidant activities of green and black teas determined by the cumene hydroperoxide/hemoglobin.methylene blue method].

    Science.gov (United States)

    Sugita, Osamu; Ishizawa, Nobuhito; Nakano, Masaharu; Matsuto, Takayuki; Okada, Masahiko

    2003-09-01

    Antioxidant activity in tea was measured by the new cumene hydroperoxide/hemoglobin.methylene blue(CHP/Hb.MB) method developed in our laboratory. Using the CHP/Hb.MB method, we investigated the activities of polyphenols(11 varieties) in order to determine their reactivity on CHP. According to the CHP/Hb.MB method, an increase in the number of hydroxyl groups in polyphenols induced high antioxidant activity. We found that this method was capable of measuring the antioxidant activity of polyphenols. Consequently, we were able to measure the antioxidant activities of heated, green, powdered and black teas by this method. The average of antioxidant activities of heated green tea was 207 nmol/ml, while that of green tea was 280 nmol/ml, powdered green tea was 481 nmol/ml and black tea was 215 nmol/ml respectively.

  5. Evaluation of the Antioxidant Activity of the Marine Pyrroloiminoquinone Makaluvamines

    Science.gov (United States)

    Alonso, Eva; Alvariño, Rebeca; Leirós, Marta; Tabudravu, Jioji N.; Feussner, Klaus; Dam, Miriam A.; Rateb, Mostafa E.; Jaspars, Marcel; Botana, Luis M.

    2016-01-01

    Makaluvamines are pyrroloiminoquinones isolated from Zyzzya sponges. Until now, they have been described as topoisomerase II inhibitors with cytotoxic effects in diverse tumor cell lines. In the present work, seven makaluvamines were tested in several antioxidant assays in primary cortical neurons and neuroblastoma cells. Among the alkaloids studied, makaluvamine J was the most active in all the assays. This compound was able to reduce the mitochondrial damage elicited by the well-known stressor H2O2. The antioxidant properties of makaluvamine J are related to an improvement of the endogenous antioxidant defenses of glutathione and catalase. SHSY5Y assays proved that this compound acts as a Nrf2 activator leading to an improvement of antioxidant defenses. A low concentration of 10 nM is able to reduce the reactive oxygen species release and maintain a correct mitochondrial function. Based on these results, non-substituted nitrogen in the pyrrole plus the presence of a p-hydroxystyryl without a double bond seems to be the most active structure with a complete antioxidant effect in neuronal cells. PMID:27801775

  6. Synergistic antioxidant activity of green tea with some herbs

    Directory of Open Access Journals (Sweden)

    Dheeraj P Jain

    2011-01-01

    Full Text Available Cardiovascular diseases, cancer, arthritis, etc. are caused by free radicals that are byproducts of metabolic pathways. Selected plants namely Vitis vinifera, Phyllanthus emblica L., Punica granatum, Cinnamomum cassia, Ginkgo biloba L., and Camellia sinensis Linn. are reported to produce antioxidant property. This study is undertaken to support the hypothesis that formulation of a polyherbal combination of these plants shows a synergistic effect with green tea. The extracts of each drug were characterized by phytochemical studies and tests for phenolics and flavonoids. In vitro antioxidant activity for individual drug and its combination was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH, superoxide, and nitric oxide free radical scavenging methods. Our results suggest that a combination of all these herbs with green tea can synergistically enhance antioxidant activity and thus lower doses of each herb with green tea may be used. Antioxidant potential of polyherbal combination was also comparable to that of standard ascorbic acid. Studies showed that selected individual plants contained abundant quantity of phenolics and flavonoids and their polyherbal combination with green tea was found to produce best antioxidant activity among all individual extracts. This will help in avoiding undesirable side effects due to higher doses of single herb.

  7. Evaluation of the Antioxidant Activity of the Marine Pyrroloiminoquinone Makaluvamines

    Directory of Open Access Journals (Sweden)

    Eva Alonso

    2016-10-01

    Full Text Available Makaluvamines are pyrroloiminoquinones isolated from Zyzzya sponges. Until now, they have been described as topoisomerase II inhibitors with cytotoxic effects in diverse tumor cell lines. In the present work, seven makaluvamines were tested in several antioxidant assays in primary cortical neurons and neuroblastoma cells. Among the alkaloids studied, makaluvamine J was the most active in all the assays. This compound was able to reduce the mitochondrial damage elicited by the well-known stressor H2O2. The antioxidant properties of makaluvamine J are related to an improvement of the endogenous antioxidant defenses of glutathione and catalase. SHSY5Y assays proved that this compound acts as a Nrf2 activator leading to an improvement of antioxidant defenses. A low concentration of 10 nM is able to reduce the reactive oxygen species release and maintain a correct mitochondrial function. Based on these results, non-substituted nitrogen in the pyrrole plus the presence of a p-hydroxystyryl without a double bond seems to be the most active structure with a complete antioxidant effect in neuronal cells.

  8. Synthesis and antioxidant activity of peptide-based ebselen analogues.

    Science.gov (United States)

    Satheeshkumar, Kandhan; Mugesh, Govindasamy

    2011-04-18

    A series of di- and tripeptide-based ebselen analogues has been synthesized. The compounds were characterized by (1)H, (13)C, and (77)Se NMR spectroscopy and mass spectral techniques. The glutathione peroxidase (GPx)-like antioxidant activity has been studied by using H(2)O(2) , tert-butyl hydroperoxide (tBuOOH), and cumene hydroperoxide (Cum-OOH) as substrates, and glutathione (GSH) as a cosubstrate. Although all the peptide-based compounds have a selenazole ring similar to that of ebselen, the GPx activity of these compounds highly depends on the nature of the peptide moiety attached to the nitrogen atom of the selenazole ring. It was observed that the introduction of a phenylalanine (Phe) amino acid residue in the N-terminal reduces the activity in all three peroxide systems. On the other hand, the introduction of aliphatic amino acid residues such as valine (Val) significantly enhances the GPx activity of the ebselen analogues. The difference in the catalytic activity of dipeptide-based ebselen derivatives can be ascribed mainly to the change in the reactivity of these compounds toward GSH and peroxide. Although the presence of the Val-Ala-CO(2) Me moiety facilitates the formation of a catalytically active selenol species, the reaction of ebselen analogues that has a Phe-Ile-CO(2) Me residue with GSH does not generate the corresponding selenol. To understand the antioxidant activity of the peptide-based ebselen analogues in the absence of GSH, these compounds were studied for their ability to inhibit peroxynitrite (PN)-mediated nitration of bovine serum albumin (BSA) and oxidation of dihydrorhodamine 123. In contrast to the GPx activity, the PN-scavenging activity of the Phe-based peptide analogues was found to be comparable to that of the Val-based compounds. However, the introduction of an additional Phe residue to the ebselen analogue that had a Val-Ala dipeptide significantly reduced the potency of the parent compound in PN-mediated nitration.

  9. Nutritional composition of boletus mushrooms from Southwest China and their antihyperglycemic and antioxidant activities.

    Science.gov (United States)

    Liu, Yuntao; Chen, Di; You, Yuxian; Zeng, Siqi; Li, Yiwen; Tang, Qianqian; Han, Guoquan; Liu, Aiping; Feng, Chaohui; Li, Cheng; Su, Yujie; Su, Zhao; Chen, Daiwen

    2016-11-15

    Thirteen samples representing five species were collected from different provinces of Southwest China, and their chemical composition, antihyperglycemic activity, and antioxidant activity were evaluated. These mushrooms had high crude protein (21.72-30.59g/100g dw) and total carbohydrate (49.18-62.58g/100g dw) contents, but low crude fat contents (1.96-7.87g/100g dw). They also accumulated notable quantities of potassium, zinc, sodium, magnesium and copper from the soil. The potassium content, in particular, was 18.75-39.21 times that found in the soil at the collection site. The natural habitat of these mushrooms, especially the mineral content of the soil, seems to have more influence on the mineral content of these mushrooms than their species. Most of the samples possessed antihyperglycemic and antioxidant activities. Suillellus luridus showed the highest antioxidant activity and antihyperglycemic activities, suggesting that S. luridus shows potential for development as a dietary nutritional supplement.

  10. Alkenes with antioxidative activities from Murraya koenigii (L.) Spreng.

    Science.gov (United States)

    Ma, Qin-Ge; Xu, Kun; Sang, Zhi-Pei; Wei, Rong-Rui; Liu, Wen-Min; Su, Ya-Lun; Yang, Jian-Bo; Wang, Ai-Guo; Ji, Teng-Fei; Li, Lu-Jun

    2016-02-01

    Four new alkenes (1-4), and six known alkenes (5-12) were isolated from Murraya koenigii (L.) Spreng. Their structures were elucidated on the basis of spectroscopic analyses and references. Compounds (1-12) were evaluated for antioxidative activities. Among them, compounds 1, 2, 4, and 7 exhibited significant antioxidative activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay with IC50=21.4-49.5 μM. The known compounds (5-12) were isolated from this plant for the first time.

  11. The effect of bioactive compounds on in vitro and in vivo antioxidant activity of different berry juices.

    Directory of Open Access Journals (Sweden)

    Ana Slatnar

    Full Text Available BACKGROUND: Berry fruit is known for its high contents of various bioactive compounds. The latter constitute of anthocyanins, flavonols and flavanols and posses high antioxidative activity. The highly dynamic antioxidant system can be evaluated in vitro and in vivo in several model organisms. These measurements represent a good approximation of the real potential of bioactive compounds in the cells of higher eucarions. The aim of the study was thus to determine in vitro and in vivo antioxidant activity of different berry juices, which reportedly contain high amounts of phenolics. METHODOLOGY/PRINCIPAL FINDINGS: Five different berry species were collected from several locations in central Slovenia and juice was extracted from each species separately. Juice was assessed for their in vitro and in vivo antioxidant activity. Phenolic profiles of berries were determined with the use of a HPLC/MS system, in vitro antioxidant activity with the DPPH radical scavenging method and in vivo antioxidative activity using Saccharomyces cerevisiae. The highest diversity of individual phenols was detected for bilberry juice. The highest in vitro antioxidant capacity was determined for blackcurrant juice. A decrease in intracellular oxidation compared to control was observed in the following order: blackcurrant < chokeberry = blueberry < bilberry. The results indicate important differences in antioxidant activity of berry juices between in vitro and in vivo studies. CONCLUSION/SIGNIFICANCE: In addition to the total content of phenolic compounds entering the cells, a key factor determining antioxidative activity of berry juices is also the ratio between the compounds. Where high content levels of anthocyanins and very low content levels of flavonols and hydroxycinnamic acids were measured a lower intracellular oxidation has been detected. Specifically, intracellular oxidation increased with higher consumption of hydroxycinnamic acids and lower consumption of

  12. Anticancer, antioxidant and antibiotic activities of mushroom Ramaria flava.

    Science.gov (United States)

    Liu, Kun; Wang, Junli; Zhao, Le; Wang, Qian

    2013-08-01

    Ramaria flava is a species of edible mushroom with some bioactivity. The anticancer, antioxidant and antibiotic activities and chemical composition of R. flava ethanol extract (EE) were evaluated. The present study exhibited that the EE displayed the strongest inhibitory activity against tumor cell MDA-MB-231 with an IC50 value of 66.54 μg/mL in three tested tumor cell lines, and the inhibition percent was 71.66% at the concentration of 200 μg/mL (MTT assay). The total phenolic compounds varied among four fractions of the EE from 6.66 to 61.01 mg gallic acid equivalent (GAE) per g dry weight. Water fraction exhibited high DPPH and OH radical-scavenging activities with low IC50 values of 5.86 and 18.08 μg/mL, respectively. Meanwhile, three phenolic compounds from water fraction were also identified by HPLC. The antibiotic activities of the EE were evaluated against three microorganisms and three fungi strains by means of the agar well diffusion method and the poisoned medium technique, respectively. The EE also showed moderate antibiotic activities. These results suggest that R. flava could hold a good potential source for human health.

  13. Organosolv ethanol lignin from hybrid poplar as a radical scavenger: relationship between lignin structure, extraction conditions, and antioxidant activity.

    Science.gov (United States)

    Pan, Xuejun; Kadla, John F; Ehara, Katsunobu; Gilkes, Neil; Saddler, Jack N

    2006-08-09

    Twenty-one organosolv ethanol lignin samples were prepared from hybrid poplar (Populus nigra xP. maximowiczii) under varied conditions with an experimental matrix designed using response surface methodology (RSM). The lignin preparations were evaluated as potential antioxidants. Results indicated that the lignins with more phenolic hydroxyl groups, less aliphatic hydroxyl groups, low molecular weight, and narrow polydispersity showed high antioxidant activity. Processing conditions affected the functional groups and molecular weight of the extracted organosolv ethanol lignins, and consequently influenced the antioxidant activity of the lignins. In general, the lignins prepared at elevated temperature, longer reaction time, increased catalyst, and diluted ethanol showed high antioxidant activity. Regression models were developed to enable the quantitative prediction of lignin characteristics and antioxidant activity based on the processing conditions.

  14. Polyphenolic Content, Antioxidant and Antimicrobial Activities of Lycium barbarum L. and Lycium chinense Mill. Leaves

    Directory of Open Access Journals (Sweden)

    Andrei Mocan

    2014-07-01

    Full Text Available This study was performed to evaluate the in vitro antioxidant and antimicrobial activities and the polyphenolic content of Lycium barbarum L. and L. chinense Mill. leaves. The different leave extracts contain important amounts of flavonoids (43.73 ± 1.43 and 61.65 ± 0.95 mg/g, respectively and showed relevant antioxidant activity, as witnessed by the quoted methods. Qualitative and quantitative analyses of target phenolic compounds were achieved using a HPLC-UV-MS method. Rutin was the dominant flavonoid in both analysed species, the highest amount being registered for L. chinense. An important amount of chlorogenic acid was determined in L. chinense and L. barbarum extracts, being more than twice as high in L. chinense than in L. barbarum. Gentisic and caffeic acids were identified only in L. barbarum, whereas kaempferol was only detected in L. chinense. The antioxidant activity was evaluated by DPPH, TEAC, hemoglobin ascorbate peroxidase activity inhibition (HAPX and inhibition of lipid peroxidation catalyzed by cytochrome c assays revealing a better antioxidant activity for the L. chinense extract. Results obtained in the antimicrobial tests revealed that L. chinense extract was more active than L. barbarum against both Gram-positive and Gram-negative bacterial strains. The results suggest that these species are valuable sources of flavonoids with relevant antioxidant and antimicrobial activities.

  15. Abscisic acid induced changes in production of primary and secondary metabolites, photosynthetic capacity, antioxidant capability, antioxidant enzymes and lipoxygenase inhibitory activity of Orthosiphon stamineus Benth.

    Science.gov (United States)

    Ibrahim, Mohd Hafiz; Jaafar, Hawa Z E

    2013-07-05

    An experiment was conducted to investigate and distinguish the relationships in the production of total phenolics, total flavonoids, soluble sugars, H2O2, O2-, phenylalanine ammonia lyase (PAL) activity, leaf gas exchange, antioxidant activity, antioxidant enzyme activity [ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD) and Lipoxygenase inhibitory activity (LOX)] under four levels of foliar abscisic acid (ABA) application (0, 2, 4, 6 µM) for 15 weeks in Orthosiphon stamineus Benth. It was found that the production of plant secondary metabolites, soluble sugars, antioxidant activity, PAL activity and LOX inhibitory activity was influenced by foliar application of ABA. As the concentration of ABA was increased from 0 to 6 µM the production of total phenolics, flavonoids, sucrose, H2O2, O2-, PAL activity and LOX inhibitory activity was enhanced. It was also observed that the antioxidant capabilities (DPPH and ORAC) were increased. This was followed by increases in production of antioxidant enzymes APX, CAT and SOD. Under high application rates of ABA the net photosynthesis and stomatal conductance was found to be reduced. The production of primary and secondary metabolites displayed a significant positive relationship with H2O2 (total phenolics, r2 = 0.877; total flavonoids, r2 = 0.812; p ≤ 0.05) and O2- (total phenolics, r2 = 0.778; total flavonoids, r2 = 0.912; p ≤ 0.05). This indicated that increased oxidative stress at high application rates of ABA, improved the production of phytochemicals.

  16. Antioxidant activities of aqueous extracts from 12 Chinese edible flowers in vitro and in vivo

    Science.gov (United States)

    Wang, Feng; Miao, Miao; Xia, Hui; Yang, Li-Gang; Wang, Shao-Kang; Sun, Gui-Ju

    2017-01-01

    ABSTRACT The antioxidant function of edible flowers have attracted increasing interest. However, information is lacking on the impact of edible flowers on oxidative injury including hypoxia-re-oxygenation and hyperlipidemia. The antioxidant activities of aqueous extracts from 12 Chinese edible flowers were assessed in four different antioxidant models, including total antioxidant capacity (TAC), oxygen radical absorbance capacity (ORAC), scavenging hydroxyl radical capacity (SHRC) and scavenging superoxide anion radical capacity (SSARC). Subsequently, the potential antioxidant effects on rat cardiac microvascular endothelial cells (rCMEC) treated with hypoxia-re-oxygenation and hyperlipidemia rats induced by high-fat diet were also evaluated. The highest TAC, ORAC, SHRC and SSARC were Lonicera japonica Thunb., Rosa rugosa Thunb., Chrysanthemum indicum L. and Rosa rugosa Thunb., respectively. Most aqueous extracts of edible flowers exhibited good antioxidant effects on injury of rCMEC induced by hypoxia-re-oxygenation. In addition, the aqueous extracts of Lonicera japonica Thunb., Carthamus tinctorius L., Magnolia officinalis Rehd. et Wils., Rosmarinus officinalis L. and Chrysanthemum morifolium Ramat. could suppress the build-up of oxidative stress by increasing serum superoxide dismutase, glutathion peroxidase, and reducing malonaldehyde concentration in hyperlipidemia rats. These findings provided scientific support for screening edible flowers as natural antioxidants and preventative treatments for oxidative stress-related diseases.

  17. Antiinflammatory, antinociceptive and antioxidant activities of Phyllanthus acidus L. extracts

    Institute of Scientific and Technical Information of China (English)

    Raja Chakraborty; Biplab De; Nayakanti Devanna; Saikat Sen

    2012-01-01

    Objective: To evaluate analgesic, anti-inflammatory and in vitro antioxidant potential and determine total phenolic, total flavonoid content of leaves extracts of Phyllanthus acidus, a folk medicinal plant of India. Methods: Anti-inflammatory activity was evaluated using carrageenan induced paw oedema, cotton pellet induced granuloma, membrane stabilizing activity method. Analgesic activity of the extracts was estimated against acetic acid induced writhing, tail immersion method, formalin test. Free radical scavenging and antioxidant potential of the extracts of Phyllanthus acidus leaves was performed using several in vitro and ex vivo assay models. Total phenolic and total flavonoid contents of the extracts were determined using standard chemical methods. Results: The extracts exhibited significant anti-inflammatory and analgesic activities at dose dependent manner. Methanol extract at a dose of 500 mg/kg showed superior activity which was comparable with the standard drugs. Ethyl acetate extract showed moderate activity while petroleum ether extract showed least activity. Total phenolic and total flavonoid content in methanol extract were 73.08±0.682 mg GAE/g and 61.28±0.062 mg QE/g respectively. The extracts possess significant antioxidant activity, methanol extract showed highest IC50 value. The contents of flavonoids and phenolic compounds could be correlated with the antioxidant, analgesic and anti-inflammatory activities observed for Phyllanthus acidus leaves. Conclusion:Our findings suggest that Phyllanthus acidus contains potential antioxidant, analgesic and anti-inflammatory compounds which could be tested as drug candidates against oxidative stress, pain and inflammation related pathological diseases.

  18. Antioxidant and antimutagenic activities of bark extract of Terminalia arjuna

    Institute of Scientific and Technical Information of China (English)

    Santosh Kumar Vaidya; Ramesh C; Nandakumar Krishnadas; Srinath Rangappa

    2010-01-01

    Objective: The alcoholic extract of stem bark of Terminalia arjuna (ALTA) was screened for antioxidant and antimutagenic (anticlastogenic) activity. Methods: Antioxidant property was determined by 1,1,Diphynyl,2-Picryl hydrazyl (DPPH) assay, super oxide radical scavenging activity, lipid peroxidation assay and total polyphenolic content was determined by Folin-Ciocalteau's reagent. Antimutagenic activity was evaluated using micronucleus test in mice. Results: The ALTA has shown potent antioxidant activity with EC50 of 2.491±0.160, 50.110±0.150 &71.000±0.250 in DPPH assay, superoxide radical scavenging activity and lipid peroxidation assay, which is comparable with ascorbic acid with EC50 of 2.471±0.140, 40.500±0.390 and 63.000±0.360 respectively. In micronucleus test, ALTA (100 & 200 mg/kg, p.o.) showed significant reduction in percentage of micronucleus in both polychromatic erythrocytes (PCE) and normochromatic erythrocytes (NCE) and also shown significant reduction in P/N ratio. Conclusions: These results suggested that ALTA possess significant antioxidant and antimutagenic activity.

  19. Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphylla

    Directory of Open Access Journals (Sweden)

    Amir Modarresi Chahardehi

    2010-01-01

    Full Text Available A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities from Pilea microphylla (Urticaceae family and including toxicity test. Antioxidant activity were tested by using DPPH free radical scavenging, also total phenolic contents and total flavonoid contents were determined. Toxicity assay carried out by using brine shrimps. Methanol extract of method I (ME I showed the highest antioxidant activity at 69.51±1.03. Chloroform extract of method I (CE I showed the highest total phenolic contents at 72.10±0.71 and chloroform extract of method II (CE II showed the highest total flavonoid contents at 60.14±0.33. The antimicrobial activity of Pilea microphylla extract was tested in vitro by using disc diffusion method and minimum inhibitory concentration (MIC. The Pilea microphylla extract showed antibacterial activity against some Gram negative and positive bacteria. The extracts did not exhibit antifungal and antiyeast activity. The hexane extract of method I (HE I was not toxic against brine shrimp (LC50 value was 3880 μg/ml. Therefore, the extracts could be suitable as antimicrobial and antioxidative agents in food industry.

  20. Antioxidant and hepatoprotective activity of Hamelia patens extracts.

    Science.gov (United States)

    Perez-Meseguer, Jonathan; Delgado-Montemayor, Cecilia; Ortíz-Torres, Tania; Salazar-Aranda, Ricardo; Cordero-Perez, Paula; de Torres, Noemí Waksman

    2016-01-01

    Hamelia patens is widely used in the traditional medicine of Mexico and Central America for the treatment of illnesses associated with inflammatory processes. In this study, antioxidant and hepatoprotective activity were assayed on the methanolic crude (ME), hexane (HE), ethyl acetate (AE), and butanol (BE) extracts of H. patens. The total phenolic content (TPC) as mg of gallic acid equivalents per g of dry extract was determined by Folin-Ciocalteu's method (ME=141.58±11.99, HE=33.96±1.13, AE=375.18±13.09, BE=132.08±3.62), and antioxidant activity by 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical-scavenging method (EC(50) ME=77.87±5.67, HE=236.64±26.32, AE=45.87±2.24, BE=50.97±0.85μg/mL). Hepatoprotective activity was evaluated through AST activity on HepG2 cells subjected to damage with CCl(4) (ME=62.5±3.41, HE=72.25±2.87, AE=63.50±4.20, BE=43.74±4.03). BE showed the greater hepatoprotective activity and a good antioxidant capacity, while HE did not show hepatoprotective or antioxidant activity. Cytotoxicity was evaluated on Vero cells cultures; none showed significant toxicity.

  1. Cooked garlic and antioxidant activity: Correlation with organosulfur compound composition.

    Science.gov (United States)

    Locatelli, D A; Nazareno, M A; Fusari, C M; Camargo, A B

    2017-04-01

    The antioxidant properties and the main beneficial organosulphur compounds of home-cooked garlic samples were studied in order to establish relationships between them. Antioxidant activity was tested by free radical scavenging against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2'-azino-bis-(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt (ABTS(+)), Fe(III) reducing ability (FRAP) and linoleic acid co-oxidation initiated by soybean lipoxygenase in a micelle system. DPPH, ABTS and FRAP assays showed the highest activity for raw garlic samples, while β-carotene bleaching assay yielded the highest activity for stir-fried garlic. Pure organosulphur compounds tested by DPPH, FRAP and β-carotene bleaching assays showed that allicin had an antiradical action mechanism, as well as iron reducing capacity; while antioxidant activity was the main mechanism for ajoenes and 2-VD. To our knowledge, this study is the first demonstration that home-cooked garlic retains its antioxidant activity, and, at the same time, elucidates the mechanisms involved in this activity.

  2. Enzyme-based online monitoring and measurement of antioxidant activity using an optical oxygen sensor coupled to an HPLC system.

    Science.gov (United States)

    Quaranta, Michela; Nugroho Prasetyo, Endry; Koren, Klaus; Nyanhongo, Gibson S; Murkovic, Michael; Klimant, Ingo; Guebitz, Georg M

    2013-03-01

    It is estimated that up to 50% of the adult population take antioxidant products on a daily basis to promote their health status. Strangely, despite the well-recognized importance of antioxidants, currently there is no international standard index for labeling owing to the lack of standardized methods for antioxidant measurement in complex products. Here, an online high-performance liquid chromatography (HPLC)-based method to detect and measure the total antioxidant capacity of antioxidant samples is presented. In this approach, complex samples containing antioxidants are separated by the HPLC system, which is further coupled to an antioxidant measuring system consisting of an optical oxygen sensor, laccase, and tetramethoxy azobismethylene quinone (TMAMQ). The antioxidants, separated via HPLC, reduce TMAMQ to syringaldazine, which is then reoxidized by laccase while simultaneously consuming O(2). The amount of consumed oxygen is directly proportional to the concentration of antioxidants and is measured by the optical oxygen sensor. The sensor is fabricated by coating a glass capillary with an oxygen-sensitive thin layer made of platinum(II) meso-tetra(4-fluorophenyl)tetrabenzoporphyrin and polystyrene, which makes real-time analysis possible (t(90) = 1.1 s in solution). Four selected antioxidants (3 mM), namely, catechin, ferulic acid, naringenin (used as a control), and Trolox, representing flavonol, hydrocinnamic acid, flavanone, and vitamin E, respectively, were injected into the online antioxidant monitoring system, separated, and then mixed with the TMAMQ/laccase solution, which resulted in oxygen consumption. This study shows that, with the use of such a system, the antioxidant activity of individual antioxidant molecules in a sample and their contribution to the total antioxidant activity of the sample can be correctly assigned.

  3. ANTIOXIDANT ACTIVITY DURING STORAGE OF APPLES SUBJECTED TO IRRADIATION

    Directory of Open Access Journals (Sweden)

    Camila Argenta Fante

    2015-06-01

    Full Text Available Nowadays, the number of studies about fruit conservation as well as the benefits of consumption of foods rich in antioxidant compounds was increased. This study aimed to quantify antioxidant compounds and their activity and to evaluate the physical and chemical changes during the post-harvest storage of Eva's apple cultivars that were subjected to gamma irradiation at doses of 0; 0.5; 1.0 and 1.5 kGy. The antioxidant activity was measured by the 2.2-diphenyl-1-picrylhydrazyl (DPPH and the β-carotene/linolenic acid methods. Levels of total phenolics, ascorbic acid, color, and soluble solids were evaluated. The results showed that, regardless of the irradiation dose, there was an increase in % SRL during post-harvest storage of Eva's apples. Low irradiation doses were able to preserve the phenolic compounds, maintain the ascorbic acid levels and avoid an increase in the soluble solids content.

  4. Phytochemical screening and antioxidant activity of Lebanese Eryngium creticum L.

    Institute of Scientific and Technical Information of China (English)

    Hussein Farhan; Fatima Malli; Hassan Rammal; Akram Hijazi; Ali Bassal; Nawal Ajouz; Bassam Badran

    2012-01-01

    Objective: To determine the phytochemical screening and quantification of total phenolics contents in fresh Eryngium creticum (E. creticum) leaves and stems extract and to evaluate its total antioxidant activity. Methods: Quantification of total phenolics contents in fresh E. creticum leaves and stems extract and evaluation of its total antioxidant activity, were done using the spectrophotometric analyses. Results: The consumption of 100 g of fresh E. creticum leaves and stems could provide antioxidants equivalent to (78.50±0.80) mg of vitamin C and (50.42±0.50) mg of vitamin C, respectively. Conclusions: From this study, it can be concluded that E. creticum can be interesting to prevent diseases directly linked to oxidative stress.

  5. Anthocyanin and antioxidant activity of snacks with coloured potato.

    Science.gov (United States)

    Nemś, Agnieszka; Pęksa, Anna; Kucharska, Alicja Z; Sokół-Łętowska, Anna; Kita, Agnieszka; Drożdż, Wioletta; Hamouz, Karel

    2015-04-01

    Coloured-fleshed potatoes of four varieties were used as raw material for coloured flour and fried snack production. The effects of thermal processes traditionally used in dried potato processing and in snack pellet manufacturing on anthocyanin profiles, total polyphenols and antioxidant properties of obtained half- and ready products were studied. There was a significant influence of potato variety on the experimental flour and snack properties. Flours with the highest antioxidant activities were obtained from Salad Blue and Herbie 26 potatoes; however, the flour prepared from the Blue Congo exhibited a much higher total polyphenol and anthocyanin content. Snacks produced with coloured flour had 2-3 times higher antioxidant activities, 40% higher contents of polyphenols, attractive colour and better expansion compared to control samples. The lowest losses of anthocyanins during snack processing were in snacks with flour from the purple-fleshed Blue Congo and red-fleshed Herbie 26. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. Hepatoprotective and antioxidant activities of Desmodium triquetrum DC

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    G A Kalyani

    2011-01-01

    Full Text Available The hepatoprotective and antioxidant activities of ethanol extract of Desmodium triquetrum DC leaf were investigated against carbon tetrachloride (1 ml/kg i.p induced hepatic damage in rats at a dose of 200 mg/kg body weight p.o. The test extract significantly (P<0.05 reduced the elevated levels of serum transaminases, alkaline phosphatase, bilirubin and reversed the antioxidant enzyme and non-enzyme levels. It dose dependently inhibited thiobarbuturic acid induced lipid peroxidation in vitro (IC 50 =59.9 μg/ml. Histopathological studies provided supportive evidence for biochemical analysis. Silymarin (25 mg/kg is a known hepatoprotective drug used as a reference drug. The results indicated that D. triquetrum has potent hepatoprotective and antioxidant activity that may be due to the presence of flavonoids in the plant.

  7. Hepatoprotective and Antioxidant Activities of Desmodium Triquetrum DC.

    Science.gov (United States)

    Kalyani, G A; Ramesh, C K; Krishna, V

    2011-07-01

    The hepatoprotective and antioxidant activities of ethanol extract of Desmodium triquetrum DC leaf were investigated against carbon tetrachloride (1 ml/kg i.p) induced hepatic damage in rats at a dose of 200 mg/kg body weight p.o. The test extract significantly (P<0.05) reduced the elevated levels of serum transaminases, alkaline phosphatase, bilirubin and reversed the antioxidant enzyme and non-enzyme levels. It dose dependently inhibited thiobarbuturic acid induced lipid peroxidation in vitro (IC(50)=59.9 μg/ml). Histopathological studies provided supportive evidence for biochemical analysis. Silymarin (25 mg/kg) is a known hepatoprotective drug used as a reference drug. The results indicated that D. triquetrum has potent hepatoprotective and antioxidant activity that may be due to the presence of flavonoids in the plant.

  8. Yucca schidigera bark: phenolic constituents and antioxidant activity.

    Science.gov (United States)

    Piacente, Sonia; Montoro, Paola; Oleszek, Wieslaw; Pizza, Cosimo

    2004-05-01

    Two new phenolic constituents with unusual spirostructures, named yuccaols D (1) and E (2), were isolated from the MeOH extract of Yucca schidigera bark. Their structures were established by spectroscopic (ESIMS and NMR) analysis. The new yuccaols D and E, along with resveratrol (3), trans-3,3',5,5'-tetrahydroxy-4'-methoxystilbene (4), yuccaols A-C (5-7), yuccaone A (8), larixinol (9), the MeOH extract of Yucca schidigera bark, and the phenolic portion of this extract, were assayed for antioxidant activity by measuring the free radical scavenging effects using two different assays, namely, the Trolox Equivalent Antioxidant Capacity (TEAC) assay and the coupled oxidation of beta-carotene and linoleic acid (autoxidation assay). The significant activities exhibited by the phenolic fraction and its constituents in both tests show the potential use of Y. schidigera as a source of antioxidant principles.

  9. Chilean Prosopis Mesocarp Flour: Phenolic Profiling and Antioxidant Activity

    Directory of Open Access Journals (Sweden)

    Guillermo Schmeda-Hirschmann

    2015-04-01

    Full Text Available In South America, the mesocarp flour of Prosopis species plays a prominent role as a food resource in arid areas. The aim of this work was the characterization of the phenolic antioxidants occurring in the pod mesocarp flour of Chilean Prosopis. Samples were collected in the Copiapo, Huasco and Elqui valleys from the north of Chile. The samples of P. chilensis flour exhibited a total phenolic content ranging between 0.82–2.57 g gallic acid equivalents/100 g fresh flour weight. The highest antioxidant activity, measured by the DPPH assay, was observed for samples from the Huasco valley. HPLC-MS/MS analysis allowed the tentative identification of eight anthocyanins and 13 phenolic compounds including flavonol glycosides, C-glycosyl flavones and ellagic acid derivatives. The antioxidant activity and the phenolic composition in the flour suggest that this ancient South American resource may have potential as a functional food.

  10. Chilean prosopis mesocarp flour: phenolic profiling and antioxidant activity.

    Science.gov (United States)

    Schmeda-Hirschmann, Guillermo; Quispe, Cristina; Soriano, Maria Del Pilar C; Theoduloz, Cristina; Jiménez-Aspée, Felipe; Pérez, Maria Jorgelina; Cuello, Ana Soledad; Isla, Maria Inés

    2015-04-17

    In South America, the mesocarp flour of Prosopis species plays a prominent role as a food resource in arid areas. The aim of this work was the characterization of the phenolic antioxidants occurring in the pod mesocarp flour of Chilean Prosopis. Samples were collected in the Copiapo, Huasco and Elqui valleys from the north of Chile. The samples of P. chilensis flour exhibited a total phenolic content ranging between 0.82-2.57 g gallic acid equivalents/100 g fresh flour weight. The highest antioxidant activity, measured by the DPPH assay, was observed for samples from the Huasco valley. HPLC-MS/MS analysis allowed the tentative identification of eight anthocyanins and 13 phenolic compounds including flavonol glycosides, C-glycosyl flavones and ellagic acid derivatives. The antioxidant activity and the phenolic composition in the flour suggest that this ancient South American resource may have potential as a functional food.

  11. Effect of extraction media on preliminary characterizations and antioxidant activities of Phellinus linteus polysaccharides.

    Science.gov (United States)

    Wang, Zhen-Bin; Pei, Juan-Juan; Ma, Hai-Le; Cai, Pan-Fu; Yan, Jing-Kun

    2014-08-30

    Three partially purified polysaccharides were extracted from Phellinus linteus mycelia using hot water, 1% (NH4)2C2O4, and 1.25M NaOH/0.05% NaBH4, and the extracts were named PL-W, PL-A, and, PL-N respectively. PL-N mainly comprised xylose and arabinose with a high molecular weight (Mw) and the highest carbohydrate and uronic acid contents. PL-W and PL-A were mainly composed of glucose with high and low Mw fractions in various ratios. All three polysaccharides existed as compact coils in aqueous solutions and exhibited strong scavenging capacity and antioxidant activities in a concentration-dependent manner. The polysaccharides also had high uronic acid and carbohydrate contents and strong antioxidant activities. The Mws, monosaccharide compositions, and chemical structures of the polysaccharides also affected their antioxidant activities. PL-A and PL-N had better antioxidant activities and could thus be developed as potential natural antioxidant agents for applications in food additives and biomedical industries.

  12. EVALUATION OF ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF MALUS DOMESTICA BORKH (APPLE AND PHASEOLUS VULGARIS L. (GREEN BEANS

    Directory of Open Access Journals (Sweden)

    A.Doss

    2012-06-01

    Full Text Available The aim of this study was to determine the antioxidant activity as well as total phenol (TPC and total flavonoid content (TFC in two fruits, apple (Malus domestica and green beans (Phaseolus vulgaris. The antioxidant activities were examined by two different methods namely DPPH free radical scavenging activity and reducing power scavenging activity. The results showed that considerable amount of TPC and TFC was present in these fruit extracts as well as these vegetables contain a vast array of different phytochemicals in their dry form. Apple showed higher antioxidant activity than green beans. Phytochemical screening revealed the presence of saponins, steroids, flavonoids and carbohydrates & glycosides in all the extracts. Overall, the present results provided basic data for choosing these fruits with high antioxidant capacity for consumption or for the development of antioxidant based medicines as value-added products.

  13. Potentiometric study of antioxidant activity: development and prospects.

    Science.gov (United States)

    Ivanova, A V; Gerasimova, E L; Brainina, Kh Z

    2015-01-01

    The increasing interest in the study of the antioxidant activity of different objects is caused by an unbalance between the formation of reactive oxygen species (ROS) and the performance of the antioxidant system in humans under certain conditions, which leads to oxidative stress and pathological states of the organism. This article presents a brief critical review of the methods that are used to measure integrated antioxidant activity (AOA). It is shown that the most promising methods for measuring AOA are electrochemical ones, particularly potentiometry, as it best fits the nature of the processes causing oxidative stress. The article gives the theoretical rational for requirements that an oxidizer of antioxidants (AO) should meet. The work presents the thermodynamic grounds for the use of an earlier proposed mediator system, kinetics of chemical reactions between AO and the mediator system. In order to confirm reliability and accuracy of the results, numerous correlation studies were conducted, aiming to compare the data obtained with the use the proposed method and independent analytical methods. The article presents the results of the potentiometric study of AOA for a variety of objects, including individual antioxidant → nutritional supplements → food → blood and blood fractions.

  14. Comparison of Antioxidant Activities of Melanin Fractions from Chestnut Shell

    Directory of Open Access Journals (Sweden)

    Zeng-Yu Yao

    2016-04-01

    Full Text Available Chestnut shell melanin can be used as a colorant and antioxidant, and fractionated into three fractions (Fr. 1, Fr. 2, and Fr. 3 with different physicochemical properties. Antioxidant activities of the fractions were comparatively evaluated for the first time. The fractions exhibited different antioxidative potential in different evaluation systems. Fr. 1, which is only soluble in alkaline water, had the strongest peroxidation inhibition and superoxide anion scavenging activity; Fr. 2, which is soluble in alkaline water and hydrophilic organic solvents but insoluble in neutral and acidic water, had the greatest power to chelate ferrous ions; and Fr. 3, which is soluble both in hydrophilic organic solvents and in water at any pH conditions, had the greatest hydroxyl (·OH and 1,1-diphenyl-2-picryl-hydrazyl (DPPH· radicals scavenging abilities, reducing power, and phenolic content. The pigment fractions were superior to butylated hydroxytolune (BHT in ·OH and DPPH· scavenging and to ethylene diamine tetraacetic acid (EDTA in the Fe2+–chelation. They were inferior to BHT in peroxidation inhibition and O2·− scavenging and reducing power. However, BHT is a synthetic antioxidant and cannot play the colorant role. The melanin fractions might be used as effective biological antioxidant colorants.

  15. Characterization of protein fractions and antioxidant activity of Chia seeds (Salvia hispanica L.

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    Kvetoslava Kačmárová

    2016-01-01

    Full Text Available Chia seed (Salvia hispanica L. is an annual herbaceous plant categorized under Lamiaceae family. Chia seeds were investigated as a source of proteins and natural antioxidants. It is a potential alternative source of high quality protein, fats, carbohydrates, high dietary fibre, vitamins and mineral elements. The objective of this study was to evaluate chia seed from protein content and antioxidant acivity and highlight the quality of this pseudocereal. A crude protein, moisture content, content of protein fractions, total antioxidant capacity (TAC and superoxide dismutase activity of chia seeds and food products containing chia seeds were determined. The protein content of chia seeds ranged from 2.9% to 4.6% dry matter from that albumins and globulins ranged from 54.6% to 62.8%. Chia is poor in a prolamines (<15%. Various chia seeds showed differences in their SOD activity and exhibited the high antiradical activity against 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS. The highest antioxidant capacity was found in sample chia seeds from Bolivia (1.46 mM TEAC.g-1 in the dry matter and the lowest values of antioxidant activity was estimated in sample chia seeds from Argentina (1.05 mM TEAC.g-1 in the dry matter. The highest SOD activity was determined in sample chia from Argentina (2191.8 U.g-1 in the dry matter. The lowest SOD activity was found in sample chia-bio from Argentina (754.0 U.g-1 in the dry matter.. It makes them potentially suitable for use in the gluten-free diet of coeliac people and it can be used as a potential ingredient in health food because of its high antioxidant activity.

  16. Variations in Tunisian borage essential oil profiles and their antioxidant activities during flowering.

    Science.gov (United States)

    Salem, Nidhal; Msaada, Kamel; Hammami, Majdi; Jday, Ahmad; Salem, Sahar; Limam, Ferid; Marzouk, Brahim

    2014-01-01

    This study was conducted to examine the chemical composition and antioxidant activity of the essential oils (EOs) of Borago officinalis stem during its flowering stage. The EO composition was characterised by high proportions of E,E-decadienal, the main compound of monoterpene hydrocarbon class, with values varying from 47.08% to 55.28% in two Tunisian regions. The region of Korba exhibited the highest scavenging activity (2.05 mg/mL) by comparison with Beja region. In all tests, the fructification stage showed the best antioxidant activity of all flowering stages.

  17. Antidiabetic and antioxidant activities ofNypa fruticans Wurmb. vinegar sample from Malaysia

    Institute of Scientific and Technical Information of China (English)

    Nor Adlin Yusoff; Mun Fei Yam; Hooi Kheng Beh; Khairul Niza Abdul Razak; Tri Widyawati; Roziahanim Mahmud; Mariam Ahmad; Mohd Zaini Asmawi

    2015-01-01

    Objective:To study the antidiabetic and antioxidant activities of nipa palm vinegar (NPV) used in traditional Malay medicine for treating diabetes.Methods:NPV was extracted using liquid-liquid extraction method and the obtained samples were subjected to antidiabetic studies using normal and streptozotocin-induced diabetic rat models whereas antidoxidant activities were investigated viain vitroantioxidant tests namely 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azinobis-3-ethylbenzothiozoline-6-sulfonic acid free radicals scavenging activities and the reducing power assay.Results:Single administration of NPV and its extracts were not effective in both normal and diabetic rats. In intraperitoneal glucose tolerance test, NPV and its aqueous extract showed significant blood glucose lowering effect. In the sub-acute study, compared with the diabetic control, aqueous extract of NPV showed the most notable blood glucose lowering effect (56.6%) and a significant improvement in serum insulin levels (79.8%, P<0.05). To assess NPV’s antioxidant activity, threein vitro antioxidant tests were employed:2,2-diphenyl-1-picryhydrazyl and 2,2’-azinobis-3-ethylbenzothiozoline-6-sulfonic acid free radical-scavenging assays, and the reducing power assay. Ethyl acetate extract had the greatest antioxidant potential and content of phenolic and flavonoid compounds. A linear positive correlation between the antioxidant parameters was observed. Chemical profiling analysis of aqueous extract of NPV revealed the presence of acetic acid (35.25%), the main active constituent which significantly contributed to the observed antidiabetic activity.Conclusions:Aqueous extract of NPV possesses antihyperglycaemic activities comparable to the metformin, while the ethyl acetate extract precipitated significant antioxidant effects attributable to its high phenolic content. These findings suggest that antioxidant compounds of NPV do not contribute much towards the overall observed antidiabetic effect.

  18. Antioxidant compounds, antioxidant activity and phenolic content in peel from three tropical fruits from Yucatan, Mexico.

    Science.gov (United States)

    Moo-Huchin, Víctor M; Moo-Huchin, Mariela I; Estrada-León, Raciel J; Cuevas-Glory, Luis; Estrada-Mota, Iván A; Ortiz-Vázquez, Elizabeth; Betancur-Ancona, David; Sauri-Duch, Enrique

    2015-01-01

    The aim of this study was to determine the antioxidant compounds, antioxidant activity and content of individual phenolic compounds of freeze-dried peel from three tropical fruits grown in Yucatan, México: purple star apple (Chrysophyllum cainito L.), yellow cashew and red cashew (Anacardium occidentale). The freeze-dried peels were good source of antioxidant compounds. ABTS and DPPH values in the peel from each fruit were 3050.95-3322.31 μM Trolox/100g dry weight (DW) or 890.19-970.01 mg of vitamin C/100 g DW, and 1579.04-1680.90 μM Trolox/100 g DW or 340.18-362.18 mg of vitamin C/100 g DW, respectively. Six phenolic compounds were identified in the peel from the tropical fruits studied: ferulic, caffeic, sinapic, gallic, ellagic and myricetin. This study demonstrated that freeze-dried peels from purple star apple, yellow cashew and red cashew, could serve as potential sources of antioxidants for use in food and pharmaceutical industries.

  19. Antioxidant Activity of Protein Hydrolysates Obtained from Common Carp (Cyprinus carpio) Discarded Roe

    DEFF Research Database (Denmark)

    Ghelichi, Sakhi; Sørensen, Ann-Dorit Moltke; García Moreno, Pedro Jesús;

    Lipid oxidation represents a severe challenge in food engineering because it deteriorates quality of foods, especially those containing high contents of polyunsaturated fatty acids (PUFAs). One way to overcome this barrier is application of synthetic antioxidants such as butylated hydroxyanisole...... (BHA), butylated hydroxytoluene (BHT), propyl gallate (PG), and tertiary butylhydroquinone (TBHQ) in PUFA-rich foods. However, recently there have been concerns over health-related risks posed by these synthetic agents. Therefore, obtaining safe antioxidants from natural sources, especially those which...... are discarded with no use, with potency to retard lipid oxidation has gained sizable attention. Therefore, the present study aimed at obtaining “green” antioxidants from discarded common carp roe via the so-called hydrolysis process by using alcalase and determining their antioxidant activity both in vitro...

  20. Antioxidant activity of bulbs and aerial parts of Crocus caspius, impact of extraction methods.

    Science.gov (United States)

    Khalili, Masomeh; Fathi, Hamed; Ebrahimzadeh, Mohammad Ali

    2016-05-01

    Crocus genus (Iridaceae) is comprises approximately 80 species. In this study in vitro antioxidant activities of extracts from C. caspius bulbs and aerial parts were investigated. Ultrasonically assisted extraction (US), percolation method (PE) and polyphenolic fraction (PP) were used. Antioxidant activities were evaluated with five different tests. Aerial parts US extract with high levels of phenol and flavonoids were the most potent extract in DPPH radical scavenging than others. Aerial parts PE extract had shown very potent reducing power, which was so better than other extracts (p<0.01). Aerial parts PP fraction showed very good Fe(2+) chelating ability. Aerial parts US extract were the most potent extract in scavenging of H(2)O(2). Bulb PP fraction with IC(50)=22.8±0.7 µg ml(-1) was the most potent fraction in nitric oxide scavenging. The results improved high levels of antioxidant activities of C. caspius bulbs and aerial parts in all tested models.

  1. Biochemical characteristics and antioxidant activity of crude and purified sulfated polysaccharides from Gracilaria fisheri.

    Science.gov (United States)

    Imjongjairak, Siriluck; Ratanakhanokchai, Khanok; Laohakunjit, Natta; Tachaapaikoon, Chakrit; Pason, Patthra; Waeonukul, Rattiya

    2016-01-01

    Sulfated polysaccharides (SPs) from Gracilaria fisheri of Thailand, which were extracted in low-temperature (25 °C) water showed the highest content of phenolic compounds compared with those extracted at high temperature (55 °C). Crude SP antioxidant activity was evaluated by measuring the DPPH free radical scavenging effect which is directly related to the level of phenolic compounds. The sulfate content, total sugar, and SPs yield were also directly related to the extraction temperature. All extracts contained galactose as a major monosaccharide. High antioxidant activity of crude SP, positively correlated with the phenolic compound contents (R(2) = 0.996) contributed by the existence of sulfate groups and phenolic compounds. In purified SP, F1 fraction exhibited strong radical scavenging ability, but it was not significantly different compared to crude SP extracted at 25 °C. This indicated that the appropriate density and distribution of sulfate groups in the SP extract showed the best antioxidant activity.

  2. Changes in nonnutritional factors and antioxidant activity during germination of nonconventional legumes.

    Science.gov (United States)

    Aguilera, Yolanda; Díaz, María Felicia; Jiménez, Tania; Benítez, Vanesa; Herrera, Teresa; Cuadrado, Carmen; Martín-Pedrosa, Mercedes; Martín-Cabrejas, María A

    2013-08-28

    The present study describes the effects of germination on nonnutritional factors and antioxidant activity in the nonconventional legumes Vigna unguiculata (cowpea), Canavalia ensiformis (jack bean), Lablab purpureus (dolichos), and Stizolobium niveum (mucuna). Protease inhibitors and lectins were detected in raw legumes and were significantly decreased during the germination. Regarding total and individual inositol phosphates (IP5-IP3), important reductions of IP6 and high increases in the rest of inositol phosphates were also detected during this process. In addition, total phenols, catechins, and proanthocyanidins increased, accompanied by an overall rise of antioxidant activity (79.6 μmol of Trolox/g of DW in the case of mucuna). Germination has been shown to be a very effective process to reduce nonnutritional factors and increase bioactive phenolic compounds and antioxidant activities of these nonconventional legumes. For this reason, they could be used as ingredients to obtain high-value legume flours for food formulation.

  3. Lipid peroxidation and antioxidant enzymes activity in avian semen.

    Science.gov (United States)

    Partyka, Agnieszka; Lukaszewicz, Ewa; Niżański, Wojciech

    2012-10-01

    The present study compared the antioxidant system and lipid peroxidation in semen of two avian species: chicken and goose. The experiment was conducted on Greenleg Partridge roosters and White Koluda(®) ganders, each represented by 10 mature males. Malondialdehyde (MDA) concentration, catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities were determined in sperm cells and seminal plasma. In gander spermatozoa, the amount of MDA was 10 times greater (Pantioxidant enzymes had greater (Pactivity in goose than chicken sperm. Catalase activity was detected in seminal plasma and spermatozoa from both studied species for the first time. In seminal plasma, the activity of GPx was two times greater (Pactivity was less (Pactivity of antioxidant defense and LPO. The greater amount of lipid peroxidation and greater activity of antioxidant enzymes in goose semen might suggest that spermatozoa were under greater oxidative stress and the enzymes were not utilized for the protection of functionally and structurally impaired cells. In turn, in fresh chicken semen a lesser activity of antioxidant enzymes accompanied with a lesser lipid peroxidation amount and good semen quality could indicate that fowl spermatozoa were under oxidative stress, but the enzymes were employed to protect and maintain sperm quality. Copyright © 2012 Elsevier B.V. All rights reserved.

  4. Antioxidant and antiproliferative activity of Granny Smith apple pomace

    Directory of Open Access Journals (Sweden)

    Savatović Slađana M.

    2008-01-01

    Full Text Available Granny Smith apple pomace was subjected to evaluation as valuable source of antioxidant and anticancer phytochemicals on the basis of its content in phenolic compounds, antioxidant and antiproliferative activity. The total cotent of phenolics, flavonoids and flavan-3-ols in apple pomace determined spectrophotometrically, was 7.02 mg/g, 0.51 mg/g and 8.80 mg/g. Major phenolics (phenolic acids, flavan-3-ols, flavonoids and dihydrochalcons in apple pomace were identified and quantified by HPLC. The antioxidant activity of apple pomace on stable 1,1-diphenyl-2-picrylhydrazyl (DPPH and reactive hydroxyl radicals, was investigated by electron spin resonance (ESR spectroscopy. The IC50 DPPH and IC50 OH values of Granny Smith apple pomace were 9.51 mg/ml and 29.17 mg/ml, respectively. The antiproliferative activities of apple pomace on cervix epitheloid carcinoma (HeLa, colon adenocarcinoma (HT-29 and breast adenocarcinoma (MCF7 cell lines were determined according to the MTT (3-(4,5-dimethylthiazol-2-yl- 2,5-diphenyltetrazolium bromide colorimetric assay. The IC50 HeLa , IC50 HT-29 and IC50 MCF7 values of Granny Smith apple pomace were 26.40 mg/ml, 22.47 mg/ml and 21.26 mg/ml, respectively. The significant correlations between antioxidant activities and antiproliferative activities were established (p<0.05.

  5. Study of Convolvulus pluricaulis for antioxidant and anticonvulsant activity.

    Science.gov (United States)

    Verma, Sristi; Sinha, Reema; Kumar, Puspendra; Amin, Faizal; Jain, Jainendra; Tanwar, Shivani

    2012-03-01

    Convolvulus pluricaulis Choisy is a perennial wild herb commonly found on sandy & rocky areas under xerophytic conditions in northern India. It is a reputed drug of ayurveda and reported to posses antioxidant, brain tonic, nervine tonic, laxative and has been used in anxiety, neurosis, epilepsy, insomnia, burning sensation, oedema and urinary disorders. In the present study, methanolic extract of whole plant of Convolvulus pluricaulis Choisy was evaluated for antioxidant activity by using 1, 1-diphenyl-2-picryl- hydrazyl (DPPH) free radical scavenging model and anticonvulsant activity by using maximal electroshock seizure model. In antioxidant activity, ascorbic acid was used as standard agent while results of anticonvulsant studies were compared with phenytoin. Results of antioxidant activity have demonstrated significant free radical scavenging effect for methanolic extract of Convolvulus pluricaulis Choisy. IC50 value of methanolic extract was observed as 41.00μg/ml as compared to 2.03μg/ml of ascorbic acid. Methanolic extract of C. pluricaulis was evaluated for anticonvulsant activity at 250, 500 and 1000mg/kg. Experimental results have shown that at the dose of 500 and 1000mg/kg, C. pluricaulis didn't abolish the hind limb extension, but reduced the mean recovery time from convulsion.

  6. Synthesis and antioxidant evaluation of isochroman-derivatives of hydroxytyrosol: structure-activity relationship.

    Science.gov (United States)

    Mateos, Raquel; Madrona, Andrés; Pereira-Caro, Gema; Domínguez, Vanessa; Cert, Rosa M A; Parrado, Juan; Sarriá, Beatriz; Bravo, Laura; Espartero, José Luis

    2015-04-15

    Isochroman-derivatives of the natural olive oil phenol hydroxytyrosol (HT) have been synthesised via Oxa-Pictet-Spengler reaction in high yields. Lipophilicity and antioxidant activity were determined to establish the structure-activity relationship of isochromans compared to HT, BHT and α-tocopherol. Antioxidant capacity was tested in two different media: bulk oils, using the Rancimat test, and brain homogenates, by measuring malondialdehyde (MDA) levels as a lipoperoxidation biomarker. In addition, other antioxidant assays (FRAP, ABTS and ORAC) were carried out. Rancimat and MDA results show that antioxidant activity was related with lipophilicity, directly in brain homogenates and inversely in the oils, in agreement with the polar paradox. Free o-diphenolic groups positively determined the activity in the oils, whereas reducing and radical-scavenging activities were related to the number of free hydroxyl moieties. BHT and α-tocopherol showed lower antioxidant activity than isochromans and HT. We conclude that HT-isochromans present significant potential as bioactive compounds.

  7. Immunomodulatory and antioxidative activity of Cordyceps militaris polysaccharides in mice.

    Science.gov (United States)

    Liu, Jing-yu; Feng, Cui-ping; Li, Xing; Chang, Ming-chang; Meng, Jun-long; Xu, Li-jing

    2016-05-01

    To evaluate the immune activation and reactive oxygen species scavenging activity of Cordyceps militaris polysaccharides (CMP) in vivo, 24 male and 24 female Kunming mice were randomly divided into four groups. The mice in the four experimental groups were administered 0 (normal control), 50, 100, or 200mg/kg/d body weight CMP via gavage. After 30 days, the viscera index, leukocyte count, differential leukocyte count, immunoglobulin (IgG) levels, and biochemical parameters were measured. The effect of CMP on the expression of tumor necrosis (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-1β in the spleens of experimental mice was investigated by real-time polymerase chain reaction. The results showed that the administration of CMP improved the immune function in mice, significantly increased the spleen and thymus indices, the spleen lymphocyte activity, the total quantity of white blood cells, and IgG function in mice serum. CMP exhibited significant antioxidative activity in mice, and decreased malondialdehyde levels in vivo. CMP upregulated the expression of TNF-α, IFN-γ, and IL-1β mRNA in high-dose groups compared to that observed for the control mice. We can thus conclude that CMP effectively improved the immune function through protection against oxidative stress. CMP thus shows potential for development as drugs and health supplements. Copyright © 2016 Elsevier B.V. All rights reserved.

  8. Antioxidant properties of phenolic Schiff bases: structure-activity relationship and mechanism of action.

    Science.gov (United States)

    Anouar, El Hassane; Raweh, Salwa; Bayach, Imene; Taha, Muhammad; Baharudin, Mohd Syukri; Di Meo, Florent; Hasan, Mizaton Hazizul; Adam, Aishah; Ismail, Nor Hadiani; Weber, Jean-Frédéric F; Trouillas, Patrick

    2013-11-01

    Phenolic Schiff bases are known for their diverse biological activities and ability to scavenge free radicals. To elucidate (1) the structure-antioxidant activity relationship of a series of thirty synthetic derivatives of 2-methoxybezohydrazide phenolic Schiff bases and (2) to determine the major mechanism involved in free radical scavenging, we used density functional theory calculations (B3P86/6-31+(d,p)) within polarizable continuum model. The results showed the importance of the bond dissociation enthalpies (BDEs) related to the first and second (BDEd) hydrogen atom transfer (intrinsic parameters) for rationalizing the antioxidant activity. In addition to the number of OH groups, the presence of a bromine substituent plays an interesting role in modulating the antioxidant activity. Theoretical thermodynamic and kinetic studies demonstrated that the free radical scavenging by these Schiff bases mainly proceeds through proton-coupled electron transfer rather than sequential proton loss electron transfer, the latter mechanism being only feasible at relatively high pH.

  9. Chemical Composition, Antimicrobial and Antioxidant Activities of Essential Oils from Organically Cultivated Fennel Cultivars

    Directory of Open Access Journals (Sweden)

    Mahmoud A. Saleh

    2011-02-01

    Full Text Available Essential oils of the fruits of three organically grown cultivars of Egyptian fennel (Foeniculum vulgare var. azoricum, Foeniculum vulgare var. dulce and Foeniculum vulgare var. vulgare were examined for their chemical constituents, antimicrobial and antioxidant activities. Gas chromatography/mass spectrometry analysis of the essential oils revealed the presence of 18 major monoterpenoids in all three cultivars but their percentage in each oil were greatly different. trans-Anethole, estragole, fenchone and limonene were highly abundant in all of the examined oils. Antioxidant activities of the essential oils were evaluated using the DPPH radical scavenging, lipid peroxidation and metal chelating assays. Essential oils from the azoricum and dulce cultivars were more effective antioxidants than that from the vulgare cultivar. Antimicrobial activities of each oil were measured against two species of fungi, two species of Gram negative and two species of Gram positive bacteria. All three cultivars showed similar antimicrobial activity.

  10. Evaluation of antioxidant and mutagenic activities of honey-sweetened cashew apple nectar.

    Science.gov (United States)

    da Silva, Robson Alves; Dihl, Rafael Rodrigues; Nascimento e Santos, Débora; de Abreu, Bianca Regina Ribas; de Lima, Alessandro; de Andrade, Heloisa Helena Rodrigues; Lehmann, Mauricio

    2013-12-01

    In vitro chemical properties and antioxidant potential and in vivo mutagenic activity of honey-sweetened cashew apple nectar (HSCAN), a beverage produced from the cashew pseudo-fruit (Anacardium occidentale L.) and of its constituents were assessed. Analytical procedures were carried out to investigate the honey used in the HSCAN preparation, and the results observed are in accordance with Brazilian legal regulations, except for diastase number. HSCAN and pulp were investigated for ascorbic acid, carotenoid, anthocyanin and total phenolic contents, and both showed high acid ascorbic concentrations. Antioxidant capacity using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and/or β-carotene/linoleic acid systems were applied and demonstrated a weak antioxidant capacity of honey and HSCAN, but cashew apple pulp demonstrated high antioxidant capacity. A weakly positive mutagenic effect of cashew pulp 20% was observed using the somatic mutation and recombination test (SMART) in Drosophila melanogaster only in the high-bioactivation (HB) cross. On the contrary, HSCAN was not mutagenic in both standard and high bioactivation crosses. HSCAN exhibited slight antioxidant activity, which could be associated with the high amount of ascorbic acid found in the samples evaluated. The beverage prepared did not induce DNA damage in somatic cells of D. melanogaster, which means that it is neither mutagenic nor recombinagenic in this test system.

  11. Antioxidant activity, total phenols and flavonoids of lichens from Venezuelan Andes.

    Directory of Open Access Journals (Sweden)

    Claudia M. Plaza

    2014-10-01

    Full Text Available Context:The biological potential of lichens has been documented through their use in traditional medicine. Secondary lichen metabolites exert a wide variety of biological actions, including their use as antioxidants. Aims:To evaluate the antioxidant activity, total phenol content, and flavonoids of four lichen fungal taxa collected in Mérida (Venezuela, and statistically evaluate the correlation between the antioxidant activity and the amount of phenols and flavonoids in the samples. Methods: Extracts were prepared with water, ethanol and dichloromethane from Cladonia aff. rappii, Cora aff. glabrata, Peltigera laciniata and Thamnolia vermicularis. The antioxidant capacity assessment was determined using DPPH• radical method and reducing power, with ascorbic acid as control. Total phenols were determined by means of the Folin-Ciocalteu method with gallic acid. Total flavonoids were estimated according to the modified Dowd method, using quercetin as standard. Results:The ethanolic extracts of the tested lichens showed the highest scavenging activity and reducing power compared to water and dichloromethane extracts at 4 mg/mL. The highest antiradical power value was found in ethanolic extract of Peltigera laciniata (2.28 mL/mgand the lowest in dichloromethane extract of Cora aff. glabrata (0.30 mL/mg. The correlation between antioxidant activity and total phenolic content was moderate. The flavonoids content of ethanolic extracts was highly significant but negative (p < 0.05. There was good correlation in dichloromethane extracts. The ethanolic extract of P. laciniata exhibited the highest antiradical activity despite showing the lowest flavonoid content. Conclusions: The ethanolic extracts of lichens tested showed to have the higher antioxidant activity and may be used as natural sources of new antioxidants.

  12. Antioxidant activity of yogurt made from milk characterized by different casein haplotypes and fortified with chestnut and sulla honeys.

    Science.gov (United States)

    Perna, Annamaria; Intaglietta, Immacolata; Simonetti, Amalia; Gambacorta, Emilio

    2014-11-01

    The aim of this work was to evaluate the antioxidant activity of yogurt made from milk characterized by different casein (CN) haplotypes (αs1-, β-, κ-CN) and fortified with chestnut and sulla honeys. The CN haplotype was determined by isoelectric focusing, whereas antioxidant activity of yogurt was measured using 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid and ferric-reducing antioxidant power. The statistical analysis showed a significant effect of the studied factors. The results showed that chestnut honey presented the highest phenolic acid and flavonoid contents, which are closely associated with its high antioxidant activity. The antioxidant activity of fortified yogurt samples was affected both by different CN haplotypes and by type of honey added. Yogurts fortified with chestnut honey showed higher antioxidant activity than those fortified with sulla honey. The different behavior observed among the fortified yogurts led us to hypothesize that the effects of protein-polyphenol complex on antioxidant activity are interactive. The results suggest that milk proteins polymorphism and polyphenols play different roles in affecting the bioavailability and the antioxidant activity of yogurt.

  13. Evaluation of Antioxidant Activity of Crataegus species Collected from Different Regions of Turkey

    Directory of Open Access Journals (Sweden)

    Mustafa Özyürek

    2012-03-01

    Full Text Available Crataegus species (Rosaceae, known as “Howthorn” have found special medicinal use for the treatment of mild heart diseases. This work aims to measure the antioxidant capacities of various Crataegus species growing in Turkey . In this study, the flowers and leaves from 52 samples belonging to 17 taxa of 14 Crataegus species naturally growing in Turkey have been investigated for their antioxidant activity/capacity. Four different methods (CUPRAC, FRAP, ABTS/Persulfate and Folin: FCR assays were used for determination of the antioxidant capacities of the samples. The leaves and the flowers of the plants were studied separately. Samples representing the same species collected from different locations were studied separately. The results have indicated that the samples differing by some minor morphological characteristics exhibit considerably different antioxidant capacities. Among the flower samples, the most effective species was C. × sinaica Boiss. nothosubsp. sinaica and among the leaf samples C. pentagyna Waldst and Kit. ex. Willd. were the most active. Generally, C. monogyna Jacq. samples have exhibited markedly high antioxidant activity. Moreover, the species collected from Bolu district (surrounded by several forest s and lake s have shown significantly high activity regardless of the species differences among the samples.

  14. Antioxidant activities and structural characterization of flavonol O-glycosides from seeds of Japanese horse chestnut (Aesculus turbinata BLUME).

    Science.gov (United States)

    Kimura, Hideto; Ogawa, Satoshi; Ishihara, Tomoe; Maruoka, Mahoko; Tokuyama-Nakai, Shota; Jisaka, Mitsuo; Yokota, Kazushige

    2017-08-01

    We attempted to evaluate the contents and distribution of antioxidants in the whole seeds, seed shells, and peeled seeds of the Japanese horse chestnut. The seed shells exhibited the highest antioxidant activities due to the presence of highly polymeric proanthocyanidins as we have reported recently. On the other hand, the peeled seeds predominantly contained flavonols such as quercetin and kaempferol at a high level of 66.7% of total polyphenols, also contributing to the predominant antioxidant activities. The instrumental analysis of the extract from the whole seeds revealed the identification of eight flavonol O-glycosides, including six compounds with quercetin and two species with kaempferol as aglycones. The isolated species exhibited different antioxidant activities depending on the types of aglycones, glycosides, and acylated moieties. The results indicate that the peeled seeds are a good source of flavonol O-glycosides serving as antioxidants to be used for food additives and dietary supplements. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Influence of In vitro Digestion on Antioxidative Activity of Coconut ...

    African Journals Online (AJOL)

    1School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang 524048, ... gastrointestinal tract, and evaluate the changes in antioxidant activity, amino acid composition and .... Pepsin (2500–3500 units/mg protein), pancreatin ..... In addition, the .... Wang B, Li Z R, Chi CF, Zhang QH, Luo HY.

  16. Antioxidant activities of selective gluten free ancient grains

    Science.gov (United States)

    Ancient grains were known for special nutritional values along with gluten free qualities. Amaranth, quinoa, teff, buckwheat flours were evaluated for pasting properties, water holding capacity, phenolic contents, and antioxidant activities (free and bound). They all had higher water holding capacit...

  17. Nanoemulsion of ethanolic extracts of propolis and its antioxidant activity

    Science.gov (United States)

    Mauludin, R.; Primaviri, D. S.; Fidrianny, I.

    2015-09-01

    Propolis contains several antioxidant compounds which can be used in topical application to protect skin against free radical and prevent skin cancer and skin aging. Ethanolic extracts of propolis (EEP) provided the greatest antioxidant activity but has very small solubility in water thus was prepared in nanoemulsion (NE). EEP contains steroid/triterpenoid, flavonoid, and saponin. EEP had the value of DPPH scavenging activity 61.14% and IC50 0.41629 ppm. The best NE formulation consisted of 26.25% Kolliphor RH40; 8.75% glycerin; 5% rice bran oil; and 3% EEP. NE was transparent, had particle size of 23.72 nm and polydispersity index of 0.338. Based on TEM morphology, NE was almost spherical and has particle size below 50 nm. NE propolis revealed to be physically stable after stability test within 63 days at 25°C and passed 6 cycles of Freeze and Thaw test without separated. NE propolis reduced around 58% of free radical DPPH similar to antioxidant activity of the original extracts. Antioxidant activity of NE propolis is relatively stable after stored for 6 weeks. NE propolis was proven to be safe by primary irritation test with the value of primary irritation index (OECD) was 0.

  18. The relationship of antioxidant components and antioxidant activity of sesame seed oil.

    Science.gov (United States)

    Wan, Yin; Li, Huixiao; Fu, Guiming; Chen, Xueyang; Chen, Feng; Xie, Mingyong

    2015-10-01

    Although sesame seed oil contains high levels of unsaturated fatty acids and even a small amount of free fatty acids in its unrefined flavored form, it shows markedly greater stability than other dietary vegetable oils. The good stability of sesame seed oil against autoxidation has been ascribed not only to its inherent lignans and tocopherols but also to browning reaction products generated when sesame seeds are roasted. Also, there is a strong synergistic effect among these components. The lignans in sesame seed oil can be categorized into two types, i.e. inherent lignans (sesamin, sesamolin) and lignans mainly formed during the oil production process (sesamol, sesamolinol, etc.). The most abundant tocopherol in sesame seed oil is γ-tocopherol. This article reviews the antioxidant activities of lignans and tocopherols as well as the browning reaction and its products in sesame seed and/or its oil. It is concluded that the composition and structure of browning reaction products and their impacts on sesame ingredients need to be further studied to better explain the remaining mysteries of sesame oil. © 2014 Society of Chemical Industry.

  19. SYNTHESIS, DOCKING STUDIES AND ANTIOXIDANT ACTIVITY OF LINEAR TETRAPEPTIDE FAYV

    Directory of Open Access Journals (Sweden)

    Sreekanth K.

    2011-07-01

    Full Text Available The linear tetrapeptide Phe-Ala-Tyr-Val (FAYV was designed based on docking results using Schrodinger Software tool. The tetrapeptide was conveniently synthesized by solution phase peptide synthesis using Dicyclohexylcarbidomide (DCC as coupling agent and triethyl amine as the base. Qikprop (a tool to predict drug like properties results showed that the ligands mostly show antihypertensive and antidiabetic properties. The compounds were evaluated for antioxidant property by using 1,1-diphenyl-2-picryl-hydrazil (DPPH. method and were found to possess significant antioxidant activity.

  20. ANTIOXIDANT, IMMUNOMODULATORY AND ANTICANCER ACTIVITIES OF EMBLICA OFFICINALIS: AN OVERVIEW

    Directory of Open Access Journals (Sweden)

    Madhuri S.

    2011-08-01

    Full Text Available Emblica officinalis (Amla is traditionally used for several diseases, and it is believed to increase the defense against diseases. It is particularly used for the treatment of cancer, diabetes, liver disorders, heart disease, ulcer, snake venom, haemorrhage, diarrhea, dysentery, anaemia and ophthalmic disorders. The antioxidant, immunomodulatory, anticancer, cytoprotective, analgesic, antimicrobial, antipyretic, antitussive and gastroprotective are the important properties of amla. Vitamin C, tannins and flavaniods present in amla have very powerful antioxidant activities. Due to rich vitamin C, amla is successfully used in the treatment of human scurvy.

  1. EVALUATION OF ANTIOXIDANT ACTIVITY OF KALANCHOE PINNATA ROOTS

    Directory of Open Access Journals (Sweden)

    Quazi Majaz

    2011-06-01

    Full Text Available The plant Kalanchoe pinnata is widely used in ayurvedic system of medicine as astringent, analgesic, carminative and also useful in diarrhea and vomiting. Naturalized throughout the hot and moist parts of India. In this first roots are subjected to pet.ether, chloroform, methanol and aqueous solvent respectively for extraction. And evaluation of antioxidant activity was done by DPPH scavenging, Nitric oxide scavenging and reducing power assay. Methanolic extract of roots of K. pinnata was found to be most effective as antioxidant as compare to other.

  2. [Constituents of Lilium candidum L. and their antioxidative activity].

    Science.gov (United States)

    Mucaji, P; Haladová, M; Eisenreichová, E; Sersen, F; Ubik, K; Grancai, D

    2007-01-01

    The paper deals with the separation and identification of a flavonoid glycoside from the petals of Lilium candidum L. and the antioxidative properties of the ethanolic extract of the flowers and selected compounds isolated from this species. The isolated flavonoid glycoside was identified as isorhamnetin-3-O-rutinoside by acid hydrolysis, TLC comparison with authentic samples, and UV and mass spectra. Isorhamnetin rutinoside was isolated from Lilium candidum L. for the first time. The antioxidative activity of the ethanolic extract of the flowers and some isolated compounds were determined using DPPH assays.

  3. Aqueous extracts of microalgae exhibit antioxidant and anticancer activities

    Institute of Scientific and Technical Information of China (English)

    Sanaa MM Shanab; Soha SM Mostafa; Emad A Shalaby; Ghada I Mahmoud

    2012-01-01

    Objective: To investigate the antioxidant and anticancer activities of aqueous extracts of nine microalgal species. Methods: Variable percentages of major secondary metabolites (total phenolic content, terpenoids and alkaloids) as well as phycobiliprotein pigments (phycocyanin, allophycocyanin and phycoerythrin) in the aqueous algal extracts were recorded. Antioxidant activity of the algal extracts was performed using 2, 2 diphenyl-1-picrylhydrazyl (DPPH) test and 2,2'-azino-bis (ethylbenzthiazoline-6-sulfonic acid (ABTS.+) radical cation assay. Anticancer efficiency of the algal water extracts was investigated against Ehrlich Ascites Carcinoma cell (EACC) and Human hepatocellular cancer cell line (HepG2). Results: Antioxidant activity of the algal extracts was performed using DPPH test and ABTS.+ radical cation assays which revealed 30.1-72.4% and 32.0-75.9% respectively. Anticancer efficiency of the algal water extracts was investigated against Ehrlich Ascites Carcinoma Cell (EACC) and Human Hepatocellular cancer cell line (HepG2) with an activity ranged 87.25% and 89.4% respectively. Culturing the promising cyanobacteria species; Nostocmuscorum and Oscillatoria sp. under nitrogen stress conditions (increasing and decreasing nitrate content of the normal BG11 medium, 1.5 g/L), increased nitrate concentration (3, 6 and 9 g/L) led to a remarkable increase in phycobilin pigments followed by an increase in both antioxidant and anticancer activities in both cyanobacterial species. While the decreased nitrate concentration (0.75, 0.37 and 0.0 g/L) induced an obvious decrease in phycobilin pigments with complete absence of allophycocyanin in case of Oscillatoria sp. Conclusions: Nitrogen starvation (0.00 g/L nitrate) induced an increase and comparable antioxidant and anticancer activities to those cultured in the highest nitrate content.

  4. Antibacterial and Antioxidant Activities of Typhonium Flagelliforme (Lodd. Blume Tuber

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    Syam Mohan

    2008-01-01

    Full Text Available Problem Statement: Multiple drug resistance in human pathogenic micro organisms has developed due to indiscriminate use of modern antimicrobial drugs generally used in the management of infectious diseases. This increases the importance of exploiting the natural sources instead modern drugs. Approach: The antibacterial and antioxidant activity of different extracts from of Typhonium flagelliforme (L. Blume tuber (family: Araceae commonly called ‘Rodent Tuber’ was assessed towards selected bacteria as well as in different antioxidant models. The antibacterial screening was carried out by disc diffusion method. Two complementary test systems, namely DPPH free radical scavenging and total phenolic compounds, were used for the antioxidant analysis. Results: Except hexane extract none of the other exacts shown anti bacterial activity against the selected strains. The hexane extract from Typhonium flagelliforme tuber had interesting activity against both the gram negative bacteria, Pseudomonas aeruginosa (11±1.0 mm diameter and Salmonella choleraesuis (12±1.1 mm diameter. The positive control, Streptomycin had shown zone of inhibition of 20±1.5 mm, 20±1.3 mm, 23±1.5 mm and 23±1.0 mm in Methicillin Resistant Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella choleraesuis and Bacillus subtilis respectively. All the extracts were subjected to screening for their possible antioxidant activity. The DPPH assay showed that the inhibitory activity of ethyl acetate (77.6±0.9 % and dichloromethane (70.5±1.7 % extracts were having comparatively admirable inhibition capacity when compared to the positive control BHT (95.3±1.3 %. Total phenolic content of all extracts was also evaluated, and dichloromethane extracts (5.21±0.82 GAE mg/g extract was superior to all other extracts, followed by hexane and ethyl acetate. Conclusion: Considering all the results collectively T. flagelliforme appears to

  5. Composition and antioxidant activity of kale (Brassica oleracea L. var. acephala raw and cooked

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    Izabela Bodziarczyk

    2012-09-01

    Full Text Available   Background. Cabbage vegetables, like Brassica group, are perceived as very valuable food products. They have a very good nutritive value, high antioxidant activity and pro-healthy potential. Especially, kale (Brassica oleraceaL. var.acephala is characterized by good nutritional and pro-healthy properties, but this vegetable is not popular in Poland. The aim of this work was to assess the chemical composition and antioxidant activity of kale variety Winterbor F1 and investigation of cooking process on selected characteristics. Material and methods. The chemical composition and antioxidant activity were determined in leaves of kale Winterbor F1 variety after three subsequent years of growing. In one season, analyses were performed on raw and cooked leaves. Results. The investigated kale was characterized by high average contents of: β-carotene (6.40 mg/100 g f.m., vitamin C (62.27 mg/100 g f.m., alimentary fiber (8.39 g/100 g f.m. and ash (2.11 g/100 g f.m.. The average amounts of nitrites (III and (V were 3.36 mg NaNO2/kg f.m. and 1206.4 mg NaNO3/kg f.m., respectively. The investigated kale contained polyphenolic compounds at average level of 574.9 mg of chlorogenic acid/100 g f.m., and its antioxidant activity measured as ABTS radical scavenging ability was 33.22 μM Trolox/g of fresh vegetable. It was observed a significant lowering of antioxidant compounds as a result of cooking. The losses of vitamin C were at about 89%, polyphenols at the level of 56%, in calculation on dry mass of the product. The highest stability was shown in the case of beta-carotene, for which the losses were at about 5%. Antioxidant activity of cooked vegetable lowered and reached the level of 38%. There were also some losses observed in macro-components from 13% for zinc to 47% for sodium. The contents of harmful nitrites and nitrates in calculation on dry mass were significantly lower as a result of cooking, by 67% and 78%, respectively. Conclusion. Winterbor F1

  6. Phenolic Content and Their Antioxidant Activity in Various Berries Cultivated in Romania

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    Zoriţa Diaconeasa

    2015-05-01

    Full Text Available Berry fruits are a rich source of phenolic compounds with health benefits.  Phenolic compounds occur in berries mainly as a variety of conjugated forms, mostly with sugars.  The aim of this work was to evaluate and compare the phenolic content and antioxidant potential in the most common fruits consumed in Romania: blueberry, blackberries, raspberry and cranberries. Folin-Ciocalteu method has been used in order to evaluate total phenolic content of analyzed berries. Antioxidant activity was determinate using ORAC assay which measures the decrease of AAPH-radical level by the scavenging action of the antioxidant substance. In addition, the vitamin C content and total tannins of the berries extracts were determined using spetophomotmetric methods. The phenolic contents and antioxidant potential of analyzed berries did not varied considerably. The highest amounts of TPC and the strongest antioxidant activities were found in blueberry and blackberries (678 GAE mg/100 g FW, 442 mg/100g FW respectively. Vitamin C content was found in higher concentration in raspberries 21.7 mg/100 g FW while the lower concentration was found in blackberry.  All berries contain higher levels of bioactive compounds such as polyphenols or tannins which are responsible for their antioxidant potential and bring their nutritional value, being highly recommended for daily consumption.

  7. In vitro and in vivo antioxidant activity of the pulp of Jatobá-do-cerrado

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    Daniela Granja ARAKAKI

    2016-03-01

    Full Text Available Abstract Oxygen metabolism in cells causes the production of free radicals, which produce damage, including changes in cell structure and function. Antioxidants are substances that, at low concentrations, slow down or prevent oxidation. Fruits and vegetables contribute to the dietary supply of these compounds. The flora of the Cerrado in Brazil has shown to have high levels of bioactive compounds. This study aimed to characterize the antioxidant activity of the pulp of jatobá-do-cerrado in vitro and in vivo.In vitro antioxidant activity of the aqueous, ethanol and aqueous acetone extracts was evaluated by the DPPH method. We determined total phenols by the Folin-Ciocalteu assay and tannins by the Folin-Denis method.In vivo antioxidant potential of the aqueous acetone extract was evaluated by the TBARS technique. The aqueous acetone extract had the highest antioxidant capacity, followed by the aqueous and ethanol extracts. The same pattern occurred in the extraction of phenols and in the extraction of tannins. In vivo administration of the aqueous acetone extract inhibited lipid peroxidation compared to the control group. The inhibition of peroxidation has increased by elevating the dosage concentration of the extracts, demonstrating a significant antioxidant potential in vivo as well as in vitro.

  8. Evaluation of Selected Culinary-Medicinal Mushrooms for Antioxidant and ACE Inhibitory Activities

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    Noorlidah Abdullah

    2012-01-01

    Full Text Available Considering the importance of diet in prevention of oxidative stress-related diseases including hypertension, this study was undertaken to evaluate the in vitro antioxidant and ACE inhibitory activities of selected culinary-medicinal mushrooms extracted by boiling in water for 30 min. Antioxidant capacity was measured using the following assays: DPPH free radical scavenging activity, β-carotene bleaching, inhibition of lipid peroxidation, reducing power ability, and cupric ion reducing antioxidant capacity (CUPRAC. Antioxidant potential of each mushroom species was calculated based on the average percentages relative to quercetin and summarized as Antioxidant Index (AI. Ganoderma lucidum (30.1%, Schizophyllum commune (27.6%, and Hericium erinaceus (17.7% showed relatively high AI. Total phenolics in these mushrooms varied between 6.19 to 63.51 mg GAE/g extract. In the ACE inhibitory assay, G. lucidum was shown to be the most potent species (IC50 = 50 μg/mL. Based on our findings, culinary-medicinal mushrooms can be considered as potential source of dietary antioxidant and ACE inhibitory agents.

  9. Antioxidant activity, polyphenol content, and related compounds in different fruit juices and homogenates prepared from 29 different pomegranate accessions.

    Science.gov (United States)

    Tzulker, Revital; Glazer, Ira; Bar-Ilan, Igal; Holland, Doron; Aviram, Michael; Amir, Rachel

    2007-11-14

    Pomegranate juice is well known for its health beneficial compounds, which can be attributed to its high level of antioxidant activity and total polyphenol content. Our objective was to study the relationships between antioxidant activity, total polyphenol content, total anthocyanins content, and the levels of four major hydrolyzable tannins in four different juices/homogenates prepared from different sections of the fruit. To this end, 29 different accessions were tested. The results showed that the antioxidant activity in aril juice correlated significantly to the total polyphenol and anthocyanin contents. However, the homogenates prepared from the whole fruit exhibited an approximately 20-fold higher antioxidant activity than the level found in the aril juice. Unlike the arils, the antioxidant level in the homogenates correlated significantly to the content of the four hydrolyzable tannins in which punicalagin is predominant, while no correlation was found to the level of anthocyanins.

  10. Investigation of antioxidant activity and lipophilicity parameters of some preservatives

    Energy Technology Data Exchange (ETDEWEB)

    Korotkova, E.I. [Department of Phys. Anal. Chem., Tomsk Polytechnic University, Lenin 30, Tomsk (Russian Federation) and Scientific Research Center ' Polyant' , Kulagina 5-29, Tomsk (Russian Federation)]. E-mail: eikor@mail.ru; Avramchik, O.A. [Department of Phys. Anal. Chem., Tomsk Polytechnic University, Lenin 30, Tomsk (Russian Federation); Scientific Research Center ' Polyant' , Kulagina 5-29, Tomsk (Russian Federation); Angelov, T.M. [Department of Quality Control, Unipharm-JSCo, Traiko Stanoev 3, Sofia (Bulgaria); Karbainov, Y.A. [Department of Phys. Anal. Chem., Tomsk Polytechnic University, Lenin 30, Tomsk (Russian Federation)

    2005-10-10

    The preservatives have been widely used in food, drugs and cosmetic products to prevent their aging and decay. Antioxidant and antimicrobial activities of some preservatives, such as benzoic acid, sorbic acid and alkyl-parabens, were considered in this work. Study of antioxidant properties of these compounds was carried out by differential pulse voltammetry together with evaluation of their influence on the kinetics of the electrochemical oxygen reduction. Mathematical models of the processes were obtained and their adequacy was estimated using design of experiment methods. As a result, the kinetic parameters of interaction between reactive oxygen species and preservatives were evaluation. Finally, the use of these substances as antimicrobials and antioxidants ensured product stability and safety has been recommended.

  11. LYCOPENE’S ANTIOXIDANT ACTIVITY IN COSMETICS MEADOW

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    Priyanka Singh

    2012-01-01

    Full Text Available Upon exposure to UV light or other oxidative stresses, photo oxidative reactions are initiated which are damaging to biomolecules and affects the integrity of cells and tissues. Photo oxidative damage plays a role in pathological processes and is involved in the development of disorders affecting skin. Carotenoids like β-carotene and lycopene act as antioxidant in photo oxidation by quenching singlet molecular oxygen and peroxy radical generated during peroxidation. There are many low molecular weight agents that serve as free radicals quenchers. Compounds capable of inhibiting free radical reactions are defined as antioxidants which have been claimed to prevent lipid peroxidation reaction in the skin and to inhibit free radicals and resulting inflammatory responses. Tomato fruit is the major source of lycopene and can be studied for its antioxidant activity in cosmetic and pharmaceutical field.

  12. Antiulcer and in vitro antioxidant activities of Jasminum grandiflorum L.

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    Umamaheswari, M; Asokkumar, K; Rathidevi, R; Sivashanmugam, A T; Subhadradevi, V; Ravi, T K

    2007-04-04

    The study was aimed at evaluating the antiulcer and antioxidant activities of 70% ethanolic axtract of leaves of Jasminum grandiflorum L. (JGLE). The leaves of Jasminum grandiflorum L. (Family: Oleaceae) is used in folk medicine for treating ulcerative stomatitis, skin diseases, ulcers, wounds, corns - a hard or soft hyperkeratosis of the sole of the human foot secondary to friction and pressure (Stedman's Medical Dictionary, 28th ed. Lippincott Williams & Wilkins, Philadelphia. p. 443), etc., Antiulcerogenic activity of JGLE (100 and 200 mg/kg, b.w., orally) was evaluated employing aspirin + pylorus ligation (APL) and alcohol (AL) induced acute gastric ulcer models and ulcer-healing activity using acetic acid-induced (AC) chronic ulcer model in rats. Both the antisecretory and cytoprotection hypothesis were evaluated. The antioxidant activity of JGLE has been assayed by using in vitro methods like 2,2-diphenyl-1-picrylhydrazylhydrate (DPPH) assay, reductive ability, superoxide anion scavenging activity, nitric oxide scavenging activity and total phenolic content, in order to explain the role of antioxidant principles in the antiulcerogenic activity of the extract. There was a significant (P<0.01) dose-dependent decrease in the ulcerative lesion index produced by all the three models in rats as compared to the standard drug famotidine (20 mg/kg, b.w. orally). The reduction in gastric fluid volume, total acidity and an increase in the pH of the gastric fluid in APL rats proved the antisecretory activity of JGLE. Additionally, JGLE completely healed the ulcer within 20 days of treatment in AC model as evidenced by histopathological studies. Like antiulcer activity, the free radical scavenging activities of JGLE depends on concentration and increased with increasing amount of the extract. These results suggest that leaves of Jasminum grandiflorum possess potential antiulcer activity, which may be attributed to its antioxidant mechanism of action.

  13. COLOUR, PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF THE SLOVAK ALIBERNET RED WINE SAMPLES

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    Daniel Bajčan

    2015-02-01

    Full Text Available Sixteen Alibernet wine samples, originated from three most important Slovak vineyard regions, were evaluated spectrophotometrically for the content of total polyphenols, content of total anthocyanins, antioxidant activity and wine colour density. The determined total polyphenol contents in observed wines ranged from 2141 to 4274 mg gallic acid per liter (average content was 3057 mg gallic acid/L and total anthocyanin contents ranged from 73.4 to 791.2 mg/L (average content was 403.4 mg/L. Determined values of antioxidant activity were within the interval 54.8 – 86.8% (average value was 74.5% and the values of wine colour density varied between 0.944 and 4.592 (average value was 2.317. The statistical evaluation of the obtained results confirmed only weak linear correlation between total polyphenol content and total anthocyanin content, however strong linear correlations between total anthocyanin content and antioxidant activity, resp. wine colour density were observed. Our results also confirmed very highly significant linear relationship between wine colour density and total polyphenol content, resp. antioxidant activity and between antioxidant activity and total phenolic content.

  14. Antioxidant Activities of Fractions of Polymeric Procyanidins from Stem Bark of Acacia confusa

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    Yi-Ming Lin

    2011-02-01

    Full Text Available The polymeric procyanidins extracted from Acacia confusa stem bark were fractionated with a step gradient of water, methanol and acetone on a Sephadex LH-20 column. The antioxidant activity of the collected fractions was investigated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH free radical scavenging and ferric reducing/antioxidant power (FRAP assays. All fractions possessed potent antioxidant activity with the highest activity observed for fraction F9. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS and reversed-phase high performance liquid chromatography (RP-HPLC analyses suggested that the collected fractions consisted primarily of oligomeric and polymeric procyanidins, with different polymer ranges and most abundant polymer size. For each fraction, catechin and epicatechin were present as both terminal and extension units, and epicatechin was the major component in the extended chain. The mean degree of polymerization (mDP of each fraction differed, ranging from 1.68 (fraction F2 to 17.31 (fraction F11. There was a relationship between antioxidant activity (IC50/DPPH and FRAP and mDP (R2DPPH = 0.861, P = 0.006 and R2FRAP = 0.608, P = 0.038, respectively. However, the highest antioxidant activity of fraction (F9 was not coincident with the maximum mDP of fraction (F11.

  15. Phenolic compounds and antioxidant activity of blueberry cultivars grown in Brazil

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    Eliseu Rodrigues

    2011-12-01

    Full Text Available The aim of this study was to evaluate the content of the phenolic compounds and anthocyanins and the antioxidant activity of blueberry (Vaccinium sp. cultivars grown in Brazil. The Folin-Ciocalteau method was applied in order to quantify the phenolic compounds and ABTS, DPPH, FRAP, and β-carotene/linoleic acid methods were applied in order to evaluated antioxidant activity. The phenolic compounds content ranged from 274.48 to 694.60 mg GAE.100 g-1 of fresh weight (FW. Anthocyanins content ranged from 40.62 to 378.31 mg.100 g-1 FW for Bluecrop and Tifblue cultivars, respectively. Antioxidant activities assessed by ABTS, DPPH and FRAP methods presented significant differences among the studied cultivars ranging from 1238.48 to 2445.96, 1014.24 to 2055.06 and 699.78 to 1740.25 µmol TEAC.100 g-1 FW, respectively. The results confirm the blueberry as a source of phenolic compounds with high antioxidant activity and also show that there are different levels of concentrations of phenolic compounds and antioxidant activity according to the cultivar and production location.

  16. Determination of some physicochemical characteristics, bioactive compounds and antioxidant activity of tropical fruits from Yucatan, Mexico.

    Science.gov (United States)

    Moo-Huchin, Víctor M; Estrada-Mota, Iván; Estrada-León, Raciel; Cuevas-Glory, Luis; Ortiz-Vázquez, Elizabeth; Vargas y Vargas, María de Lourdes; Betancur-Ancona, David; Sauri-Duch, Enrique

    2014-01-01

    The aim to the study was to determine the physicochemical composition, bioactive compounds and antioxidant activity of fruits from Yucatan, Mexico such as star apple, cashew, mombin, mamey sapote, white sapote, sugar apple, sapodilla, dragon fruit, nance, ilama, custard apple, mamoncillo and black sapote. The physicochemical characteristics were different between fruits and were good sources of bioactive compounds. The edible part with the highest values of antioxidant activity were mamoncillo, star apple, mombin, cashew, white sapote, ilama, custard apple, sugar apple, and nance. Total soluble phenols content showed a correlation with antioxidant activity by ABTS (R=0.52, P⩽0.05) and DPPH (R=0.43, P⩽0.05). A high correlation was obtained between the two assays (ABTS and DPPH) used to measure antioxidant activity in the tropical fruit species under study (R=0.82, P⩽0.05). The results show promising perspectives for the exploitation and use of tropical fruits studied with significant levels of nutrients and antioxidant activity. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. Measurement of antioxidant activity with trifluoperazine dihydrochloride radical cation

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    M.N. Asghar

    2008-06-01

    Full Text Available A novel, rapid and cost-effective trifluoperazine dihydrochloride (TFPH decolorization assay is described for the screening of antioxidant activity. A chromogenic reaction between TFPH and potassium persulfate at low pH produces an orange-red radical cation with maximum absorption at 502 nm in its first-order derivative spectrum. TFPH was dissolved in distilled water to give a 100 mM solution. The TFPH radical cation solution was made by reacting 0.5 mL of the solution with K2S2O8 (final concentration: 0.1 mM and diluting to 100 mL with 4 M H2SO4 solution. A linear inhibition of color production was observed with linearly increasing amounts of antioxidants, with correlation coefficients (R² ranging from 0.999 to 0.983. The antioxidant capacity of standard solutions of an antioxidant was evaluated by comparing with the inhibition curve using Trolox as the standard. Comparison of antioxidant capacity determined with this newly developed TFPH assay and with the well-known 2,2'-azinobis-[3-ethylbenzthiazoline-6-sulfonic acid] (ABTS-persulfate decolorization assay indicated the efficacy and sensitivity of the procedure. The proposed assay is less expensive (costs about US$4 per 100 assays and requires only 20 min for preparation of radical cation solution in comparison with ABTS assay, in which almost 12-16 h are required for preparation of a stable ABTS radical cation solution. The present assay has the advantage over ABTS assay that it can be used to measure the antioxidant activity of the samples, which are naturally found at a pH as low as 1, because the radical cation itself has been stabilized at low pH.

  18. Antioxidative and antiinflammatory activities of quercetin-loaded silica nanoparticles.

    Science.gov (United States)

    Lee, Ga Hyun; Lee, Sung June; Jeong, Sang Won; Kim, Hyun-Chul; Park, Ga Young; Lee, Se Geun; Choi, Jin Hyun

    2016-07-01

    Utilizing the biological activities of compounds by encapsulating natural components in stable nanoparticles is an important strategy for a variety of biomedical and healthcare applications. In this study, quercetin-loaded silica nanoparticles were synthesized using an oil-in-water microemulsion method, which is a suitable system for producing functional nanoparticles of controlled size and shape. The resulting quercetin-loaded silica nanoparticles were spherical, highly monodispersed, and stable in an aqueous system. Superoxide radical scavenging effects were found for the quercetin-loaded silica nanoparticles as well as free quercetin. The quercetin-loaded silica nanoparticles showed cell viability comparable to that of the controls. The amounts of proinflammatory cytokines produced by macrophages, such as interleukin 1 beta, interleukin 6, and tumor necrosis factor alpha, were reduced significantly for the quercetin-loaded silica nanoparticles. These results suggest that the antioxidative and antiinflammatory activities of quercetin are maintained after encapsulation in silica. Silica nanoparticles can be used for the effective and stable incorporation of biologically active natural components into composite biomaterials.

  19. Antioxidant activities of the constituents of Picris echoides

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    MILUTIN STEFANOVIC

    2002-01-01

    Full Text Available Some flavonoids with antioxidant properties from the aerial parts of the plant species Picris echoides (family Asteraceae were identified. Upon chromatography, the ethyl acetate extract afforded flavonoids, such as: flavone apigenin (1 and its glucoside, cosmosiin (2, as well as common plant constituents from this family, flavonol galetin (3, 3,4’,5,6,7-pentahydroxyflavone and 4,4’,6,7-tetrahydroxyaurone (4. The structure of the aurone 4 has not been described so far in the literature and presented a very rare type of aurone skeleton. The structures of the isolated compounds were determined by interpretation of their physical and spectral data. The antioxidant activities of different extracts from Picris echoides were measured by the Schaal oven test at 60ºC and by the Rancimat method at 100ºC. Water/ethanol extracts (2:8, v/v, in concentrations of 0.02 and 0.05 %, showed lower activity than commercial tocopherol (Tch. On the contrary, the purified ethyl acetate extracts showed a strong concentration-dependent antioxidant effect. The investigation demonstrated that galetin was the main flavonol from this origin. According to the results of the two methods, galetin (3 showed a two-fold better activity than did Tch and a lower activity than did butylated hydroxyanisole (BHA. The aurone 4 exhibited significantly lower antioxidant activity than did galetin at the same concentration level. Thus, the plant species P. echoides is a new and favorable source of natural lipid antioxidants.

  20. Total antioxidant activity of yacon tubers cultivated in Brazil

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    Juciane Abreu Ribeiro Pereira

    Full Text Available ABSTRACT Yacon (Smallanthus sonchifolius is a tuberous root from the Andean region in the South America rich em water, fructooligosaccharides and phenolic compounds, some of which are natural antioxidants and may help prevent the deleterious action of free radicals in the body. The yacon has attracted much attention due to their potential health benefits to humans. In this study the levels of total phenolics, tannins, phenolic acids, and total antioxidant activity were measured in the peel and pulp of yacon tubers both in the fresh and flour forms. The flours of yacon presented higher concentrations of total phenolics and tannins, especially peel flour. The yacon pulp flour stood out as the main source of phenolic acids, mainly caffeic and chlorogenic acid. The total antioxidant activity assessed by DPPH (2,2-Diphenyl-1-picrylhydrazyl and ABTS (2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid assays was higher in the yacon peel flour. The total antioxidant activity was correlated with the total phenolic content and tannins by the DPPH and ABTS assays. These results suggest that yacon can be used as an alternative food source of phenolic compounds that help prevent degenerative processes caused by oxidative stress, especially in the flours form.

  1. Production, solubility and antioxidant activity of curcumin nanosuspension

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    Deivis de Moraes Carvalho

    2015-03-01

    Full Text Available Curcumin is a powerful bioactive agent and natural antioxidant, but it is practically water-insoluble and has low bioavailability; a possible solution to this obstacle would be formulations of curcumin nanoparticles. Surfactants such as tween 80 can be used to stabilize low-solubility molecules preventing particle aggregation. The objectives of this study were the preparation of a suspension with curcumin nanoparticles in tween 80, the testing of pure curcumin solubility and of a simple mixture of curcumin with tween 80 and nanosuspension in water and ethanol as solvents, and finally the assessment of the antioxidant activity. We prepared the nanosuspension by injecting a curcumin solution in dichloromethane at low flow in water with tween 80 under heating and ultrasound. The analysis of particles size was conducted through dynamic light scattering; the non-degradation of curcumin was verified through thin-layer chromatography. The analyses of antioxidant activity were carried out according to the DPPH method. The method applied to reduce the particles size was efficient. Both the curcumin suspension and nanosuspension in tween 80 increased its solubility. Curcumin and the formulations presented antioxidant activity.

  2. IN-VITRO ANTIOXIDANT ACTIVITY OF GOSSYPIUM HERBACEUM LINN.

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    Sharma Pravesh Kumar

    2011-07-01

    Full Text Available The present study investigated In-vitro antioxidant activity of hydroalcoholic leaves extract (70: 30 of Gossypium herbaceum Linn. to provide scientific basis for traditional usage of this plant. The In-vitro antioxidant activity was evaluated by determining reducing power, total flavonoids and total phenolic contents using standard assay methods. The ability of the extract to scavenge 1, 1-diphenyl-2-picrylhydrazyl (DPPH was also assessed using spectroscopic method. Ascorbic acid was used as standard antioxidant and positive control. The DPPH radical scavenging activity of the extract was increased with the increasing concentration. The IC50 values for DPPH assay of Gossypium herbaceum and ascorbic acid were found to be 44.69µg/ml and 13.80µg/ml respectively. The reducing power of the extract was found to be concentration dependent. Total flavonoid content in the extract was found to be 410 ± 0.74 mg Quercetin equivalents/g of dry material. Total phenolic content in Gossypium herbaceum was found to be 5.86 ± 0.75 mg Gallic acid equivalents/g of dry material. The results obtained from this study indicate that Gossypium herbaceum is a potential source of antioxidants and thus could prevent many radical diseases.

  3. Seasonal Difference in Antioxidant Capacity and Active Compounds Contents of Eucommia ulmoides Oliver Leaf

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    Jingfang Zhang

    2013-02-01

    Full Text Available Leaf of Eucommia ulmoides Oliver (EU is a Traditional Chinese Medicine and a functional food in China. Antioxidant contents of EU leaves, which were collected monthly during the period of May–October in three years, were determined. Samples’ antioxidant capacity was characterized by DPPH radical scavenging activity, hydroxyl radical scavenging activity, ferrous chelating ability, and antioxidant capacity in linoleic acid emulsion and in rapeseed oil assays. The results showed that contents of some active compounds and antioxidant activity were related to a certain time of the year. Samples collected in August showed high content of phenolics, and the samples collected in May contained higher amount of flavonoids than other samples. Leaves collected in May or June exhibited high contents of rutin, quercetin, geniposidic acid and aucubin. The August leaves showed stable and high DPPH radical scavenging activity, and ferrous chelating ability. May samples showed strong inhibitory effects on oxidation of rapeseed oil and linoleic acid. The DPPH radical scavenging activity was related to the total phenolics content. Flavonoids played an important role in the inhibitory effects on rapeseed oil and linoleic acid oxidation. Therefore, August and May were indicated as the best months to harvest EU leaves for industry.

  4. Protein precipitating capacity and antioxidant activity of Turkish Tombul hazelnut phenolic extract and its fractions.

    Science.gov (United States)

    Pelvan Pelitli, Ebru; Janiak, Michał Adam; Amarowicz, Ryszard; Alasalvar, Cesarettin

    2017-03-01

    Natural (raw) hazelnut was extracted with 80% (v/v) acetone to obtain crude phenolic extract that was then fractionated for elution of low-molecular weight (LMW) and high-molecular weight (HMW) fractions. LMW fraction was further purified (LWM-FP) to remove sugars and organic acids. The crude extract and its fractions were determined by measuring their protein precipitating capacity (PPC) using two different proteins [bovine serum albumin (BSA) and gelatin], molecular weights, total phenolics, condensed tannins, and various antioxidant activities. Significant differences (p<0.05) existed in the contents of total phenolics, condensed tannins, antioxidant activities, and PPC among the crude extract and fractions, albeit to different extends. BSA and gelatin was effectively precipitated by HMW fraction. HMW fraction had the highest total phenolics, condensed tannins, and antioxidant activities, followed by crude extract, LWM-FP, and LMW, respectively. The present study suggests that HMW fraction could be utilised as a source of polyphenols for the food industry.

  5. Effects of Copper Pollution on the Phenolic Compound Content, Color, and Antioxidant Activity of Wine.

    Science.gov (United States)

    Sun, Xiangyu; Ma, Tingting; Han, Luyang; Huang, Weidong; Zhan, Jicheng

    2017-05-03

    The effects of copper pollution on the polyphenol content, color, and antioxidant activity of wine, as well as correlations among these factors, were investigated. Copper had clear influences on wine polyphenol content. At low copper concentrations, the concentrations of nearly all polyphenols increased, and the antioxidant activity values of the wine also increased. When the copper concentration reached the lowest level of the medium copper range (9.6~16 mg/L), most of the indices also improved. When the copper concentrations reached the latter part of the medium copper range (19.2 and 22.4 mg/L), many of the tested indices began to decrease. Furthermore, when the copper concentration reached the high ranges (32, 64, and 96 mg/L), the polyphenol content, CIELAB color parameters, and antioxidant activity of wine were substantially decreased, indicating the need to control increasing copper content in grape must.

  6. Polyphenol Content and Antioxidant Activity of Fourteen Wild Edible Fruits from Burkina Faso

    Directory of Open Access Journals (Sweden)

    Odile G. Nacoulma

    2008-03-01

    Full Text Available A total of fourteen (14 species of wild edible fruits from Burkina Faso wereanalyzed for their phenolic and flavonoid contents, and their antioxidant activities usingthe DPPH, FRAP and ABTS methods. The data obtained show that the total phenolic andtotal flavonoid levels were significantly higher in the acetone than in the methanol extracts.Detarium microcarpum fruit had the highest phenolic and the highest flavonoid content,followed by that of Adansonia digitata, Ziziphus mauritiana, Ximenia americana andLannea microcarpa. Significant amounts of total phenolics were also detected in the otherfruit species in the following order of decreasing levels: Tamarindus indica > Sclerocaryabirrea > Dialium guineense > Gardenia erubescens > Diospyros mespiliformis > Parkiabiglobosa > Ficus sycomorus > Vitellaria paradoxa. Detarium microcarpum fruit alsoshowed the highest antioxidant activity using the three antioxidant assays. Fruits with highantioxidant activities were also found to possess high phenolic and flavonoid contents.There was a strong correlation between total phenolic and flavonoid levels and antioxidantactivities.

  7. HPLC/MS analysis of polyphenols, antioxidant and antimicrobial activities of Artabotrys hildebrandtii O. Hffm. extracts.

    Science.gov (United States)

    Andriamadio, Julio Hervé; Rasoanaivo, Léa Herilala; Benedec, Daniela; Vlase, Laurian; Gheldiu, Ana-Maria; Duma, Mihaela; Toiu, Anca; Raharisololalao, Amélie; Oniga, Ilioara

    2015-01-01

    The purpose of this work was to evaluate chemical constituents, antioxidant and antimicrobial activities of Artabotrys hildebrandtii, an endemic medicinal plant from Madagascar. Ethanol extracts from the leaves and stem bark were tested to evaluate DPPH free radical scavenging, using butylated hydroxytoluene and quercetin as standard antioxidants. An high-performance liquid chromatography/mass spectrometry method was developed to investigate the presence of phenolic compounds in the studied samples; gentisic acid, chlorogenic acid, hyperoside, isoquercitrin, rutin, quercitrin, quercetol, apigenin and luteolin were identified. Total polyphenolic content was determined by a spectrophotometric method using Folin-Ciocâlteu reagent. Results showed the efficiency of A. hildebrandtii leaves extract against strains of Staphylococcus aureus and Listeria monocytogenes, as the inhibitory activity is more powerful compared to Gentamicin, used as the standard drug. The leaves of A. hildebrandtii can be considered an important source of polyphenols, especially of rutin, with good antioxidant and antimicrobial activities.

  8. Effect of phosphorylation on antioxidant activities of pumpkin (Cucurbita pepo, Lady godiva) polysaccharide.

    Science.gov (United States)

    Song, Yi; Ni, Yuanying; Hu, Xiaosong; Li, Quanhong

    2015-11-01

    Phosphorylated derivatives of pumpkin polysaccharide with different degree of substitution were synthesized using POCl3 and pyridine. Antioxidant activities and cytoprotective effects of unmodified polysaccharide and phosphorylated derivatives were investigated employing various in vitro systems. Results showed that high ratio of POCl3/pyridine could increase the degree of substitution and no remarkable degradation occurred in the phosphorylation process. Characteristic absorption of phosphorylation appeared both in the IR and (31)P NMR spectrum. The df values between 2.27 and 2.55 indicated the relatively expanded conformation of the phosphorylated derivatives. All the phosphorylated polysaccharides exhibited higher antioxidant activities. H2O2-induced oxidative damages on rat thymic lymphocyte were also prevented by the derivatives. In general, phosphorylation could improve the antioxidant activities of pumpkin polysaccharide both in vitro and in a cell system.

  9. Antioxidant activity of dorzolamide/timolol fixed combination in neuroprotective therapy in glaucoma

    Directory of Open Access Journals (Sweden)

    N. I. Kurysheva

    2014-07-01

    Full Text Available Purpose: to study the antioxidant activity of carbonic anhydrase inhibitors and timolol fixed combinations and to compare it with other fixed combinations.Methods: Antioxidant activity (AOA of dorzolamide/timolol (Cosopt, dorzolamide/timolol (Dorzopt Plus, latanoprost/timolol, brimonidine/timolol, travoprost/timolol and bimatoprost/timolol fixed combinations was measured in vitro using the model of oxida- tive hemolysis.Results: Dorzolamide/timolol (Cosopt AOA was higher than that of other fixed combinations and increased with the quantity of the drugs added to the model system: 40%, 52% and 75% in 30 μl, 60 μl and 90 μl respectively.Conclusion: these findings suggest that dorzolamide/timolol fixed combination has potential advantages over the other fixed combinations due to its high antioxidant activity and might be used as the neuroptotective agent for glaucoma treatment.

  10. Nutritional Composition, α-Glucosidase Inhibitory and Antioxidant Activities of Ophiopogon japonicus Tubers

    Directory of Open Access Journals (Sweden)

    Yancui Wang

    2015-01-01

    Full Text Available Ophiopogon japonicus tubers have been widely used as food and traditional Chinese medicine in China. However, their nutritional composition has not been fully reported yet. This study aimed to analyze the nutritional composition of O. japonicus tubers. The α-glucosidase inhibitory and antioxidant activities of the extracts obtained from O. japonicus tubers were also evaluated by in vitro assays. The results indicated that O. japonicus tubers are rich in carbohydrates, proteins, minerals, and amino acids. Among four extracts, the n-butanol fraction (nBF and chloroform/methanol extract (CME of O. japonicus tubers had high amounts of total phenolic and flavonoid contents and exhibited good α-glucosidase inhibitory and antioxidant activities. The α-glucosidase inhibition of nBF was higher than acarbose. Overall, O. japonicus tubers are full of nutritional compounds and have good α-glucosidase inhibitory and antioxidant activities.

  11. Antioxidant and Antityrosinase Activity of Flemingia macrophylla and Glycine tomentella Roots

    Directory of Open Access Journals (Sweden)

    Bor-Sen Wang

    2012-01-01

    Full Text Available The antioxidant and antityrosinase activities of the water extract of Flemingia macrophylla root (WEFM were investigated. The results showed that WEFM exhibited radical scavenging and reducing activities, as well as ferrous ion chelating property. In addition, WEFM also protected phospholipids against oxidation, indicating that WEFM could protect biomolecules from oxidative damage. Meanwhile, in the range of 50–100 μg/mL, the tyrosinase inhibitory activity of WEFM increased with an increase in sample concentration and was superior to that of the water extract of Glycine tomentella root (WEGT. A high performance liquid chromatography analysis was used to determine the phenolic components, revealing that daidzin, daidzein, genistin, and genistein were present in WEFM and WEGT. Acting as an antioxidant and a tyrosinase inhibitor, these bioactive constituents could contribute to the protective effects of WEFM. Overall, the results showed that WEFM might serve as a natural antioxidant and tyrosinase inhibitor.

  12. Antioxidant activity of dorzolamide/timolol fixed combination in neuroprotective therapy in glaucoma

    Directory of Open Access Journals (Sweden)

    N. I. Kurysheva

    2012-01-01

    Full Text Available Purpose: to study the antioxidant activity of carbonic anhydrase inhibitors and timolol fixed combinations and to compare it with other fixed combinations.Methods: Antioxidant activity (AOA of dorzolamide/timolol (Cosopt, dorzolamide/timolol (Dorzopt Plus, latanoprost/timolol, brimonidine/timolol, travoprost/timolol and bimatoprost/timolol fixed combinations was measured in vitro using the model of oxida- tive hemolysis.Results: Dorzolamide/timolol (Cosopt AOA was higher than that of other fixed combinations and increased with the quantity of the drugs added to the model system: 40%, 52% and 75% in 30 μl, 60 μl and 90 μl respectively.Conclusion: these findings suggest that dorzolamide/timolol fixed combination has potential advantages over the other fixed combinations due to its high antioxidant activity and might be used as the neuroptotective agent for glaucoma treatment.

  13. Synthesis of Stilbene Derivatives: A Comparative Study of their Antioxidant Activities.

    Science.gov (United States)

    Romero, Miguel A; González-Delgado, José A; Arteaga, Jesüs F

    2015-07-01

    A series of structurally simple compounds belonging to thestilbene family were synthesized by means of a Ti(III)-mediated methodology that allows access, in an efficient manner, to derivatives of dihydrostilbene, E-stilbene, and stilbene oxide, with high yields. The antioxidant activity of these compounds has been evaluated by means of two electrochemical assays, which provide complementary information, showing that the majority of these stilbene analogs exhibit significant antioxidant activity dependent on the electronic structure and functionalization of the molecule in each case.

  14. Phytochemical, Anti-oxidant and Anthelmintic activities of various leaf extracts of Flacourtia sepiaria Roxb

    Directory of Open Access Journals (Sweden)

    M Sreejith

    2013-12-01

    Conclusions: The results of the present study indicate that the leaf extracts of Flacourtia sepiaria exhibited strong antioxidant activity and possess significant anthelmintic activity and thus it is a good source of antioxidant and anthelmintic constituents.

  15. THE ANTIOXIDANT ACTIVITY OF THE PRODUCTS OF HYDROLYSIS OF NATURAL POLYMERS (MANNAN AND FUCOIDAN

    Directory of Open Access Journals (Sweden)

    D. A. Cherenkov

    2012-01-01

    Full Text Available The antioxidant activity of oligosaccharides, obtained from mild acid and fermentative mannan and fucoidan hydrolysis was investigated. Dependence of manno- and fucoooligosaccharides antioxidant activity on hydrolysis conditions was shown.

  16. TLC Screening for Antioxidant Activity of Extracts from Fifteen Bamboo Species and Identification of Antioxidant Flavone Glycosides from Leaves of Bambusa. textilis McClure

    OpenAIRE

    Jia Sun; Feng Tang; Yong-De Yue; Jin Wang

    2012-01-01

    Interest in the antioxidant activity of bamboo leaves is growing. To discover new sources of natural antioxidants, a TLC bioautography method combined with a new image processing method was developed to evaluate the antioxidant activity of leaf extracts from 15 different species of bamboo. The results showed that the methanolic extract of Bambusa. textilis McClure possessed the highest antioxidant activity among the selected bamboo species. To rapidly identify the antioxidant compounds, the c...

  17. Phytochemical analysis and antioxidant activities of Trigona Apicalis propolis extract

    Science.gov (United States)

    Rosli, Nur Liyana; Roslan, Husniyati; Omar, Eshaifol Azam; Mokhtar, Norehan; Hapit, Nor Hussaini Abdul; Asem, Nornaimah

    2016-12-01

    Propolis is a resinous substance found in beehives. It provides beneficial effects on human health and has been used to treat many diseases since ancient times. The objectives of this study were to analyze the phytochemical profile of propolis derived from local T. apicalis species and its antioxidant activities. The ethanolic extract of propolis was subjected to HPLC analysis to analyze its phytochemical profile. The propolis extract was later tested for antioxidant capacities by using DPPH radical scavenging assay. TPC and TFC were performed to determine the correlation with its antioxidant activities. TEAC for each serial dilution sample was 2621.15 (4.76 mg/mL), 2050.85 (2.38 mg/mL), 1883.27 (1.19 mg/mL), 1562.67 (0.59 mg/mL), 1327.82 (0.29 mg/mL), 1164.49 (0.15 mg/mL), 983.27 (0.07 mg/mL), and 944.79 (0.04 mg/mL). The results demonstrated that the antioxidant activities of propolis extract were dose dependent. The IC50 of propolis for DPPH assay was 4.27 mg/ml. Correlation values of TPC and TFC against DPPH indicate that the antioxidant activities of propolis extract used in this study could be mainly influenced by the phenolic and flavonoid contents. These findings highlighted the importance of quality analysis in order to ensure the consistency of biological effects or therapy of a natural product, such as propolis.

  18. Extraction and antioxidant activity of flavonoids of Morus nigra.

    Science.gov (United States)

    Feng, Rui-Zhang; Wang, Qin; Tong, Wen-Zhi; Xiong, Juan; Wei, Qin; Zhou, Wan-Hai; Yin, Zhong-Qiong; Yin, Xiao-Ya; Wang, Li-Ying; Chen, Ya-Qin; Lai, Yong-Hong; Huang, Hong-Yan; Luo, Qiao-Li; Wang, Lu; Jia, Ren-Yong; Song, Xu; Zou, Yuan-Feng; Li, Li-Xia

    2015-01-01

    Morus nigra has a long history of medicinal use in Chinese medicine, but the study on it is limited, the flavonoids are one of the main biological active substances. In this study, the Morus nigra flavonoids were extracted by ultrasonic and antioxidant activities both in vitro and in vivo were measured. The results showed that hydroxyl radicals clearance rate and superoxide radical anion clearance rate in vitro increased with the concentration of the total flavonoids in the range of 0-1.05 mg/mL and the maximum clearance rate was 80.33% and 87.69%, respectively. After mice were treated with flavonoids, the content of malonaldehyde (MDA) in serum and liver decreased; the activities of superoxide dismutase (SOD) in serum and liver, catalase (CAT) in liver and glutathione peroxidase (GSH-PX) in blood and liver increased; Langhans cells increased in spleen. These results revealed that the Morus nigra flavonoids possessed strong antioxidant activity.

  19. Studies on the antioxidant activities of Desmodium gangeticum.

    Science.gov (United States)

    Govindarajan, Raghavan; Rastogi, Subha; Vijayakumar, Madhavan; Shirwaikar, Annie; Rawat, Ajay Kumar Singh; Mehrotra, Shanta; Pushpangadan, Palpu

    2003-10-01

    Desmodium gangeticum is herbal species which is widely used in the indigenous system of medicine and is reported to contain flavone and isoflavanoid glycosides. In view of its wide use and it's chemical composition, this study was aimed at examining the antioxidant activity of the extract of D. gangeticum. The extract was studied for diphenyl picryl hydrazyl (DPPH), nitric oxide, ferryl-bipyridyl and hypochlorous acid scavenging activity along with lipid peroxidation. Nitric oxide was generated using sodium nitroprusside and was studied using Griess reagent. In order to study the iron chelating capacity of the extract, the percentage ferryl-bipyridyl inhibition was studied. Hypochlorous acid scavenging activity was tested by measuring the inhibition of 5-thio-2-nitrobenzoic acid oxidation. The extract was also studied for lipid peroxidation assay by thiobarbituric acid-reactive substances (TBARS) method using rat brain homogenate. The results indicate that D. gangeticum extract has potent antioxidant activity.

  20. Antibacterial Potential and Antioxidant Activity of Polyphenols of Sesbania grandiflora

    Directory of Open Access Journals (Sweden)

    M.B. Ouattara

    2011-07-01

    Full Text Available The antibacterial and antioxidant activity of Sesbanial grandiflora used in traditional pharmacopeia in Burkina Faso and elsewhere was evaluated. Aqueous, methanolic and hydro-acetone extractions were carried out on the leaves, stems, and granules, pods of fruit and roots of the plant. The phytochimic groups were identified by the tests of characterization, then quantified by the tests of proportioning of total phenolics, flavonoides and tanins. Specific compounds to these phytochimic groups were also identified by the analysis in Thin layer chromatography among which the gallic acid, the caffeic acid, Kaempferol, Quercetin, Rutin. An important antioxidant activity of the same extracts was evaluated by the test of reduction of the stable radical, the 2,2-diphényl-1-picrylhydrazyl (DPPH* and the test of reduction of iron (FRAP. This activity is related to phenolic compounds contained in the extracts. Extracts also expressed a good antibacterial activity.

  1. Chemical characterization, antioxidant and cytotoxic activities of Brazilian red propolis.

    Science.gov (United States)

    Frozza, Caroline Olivieri da Silva; Garcia, Charlene Silvestrin Celi; Gambato, Gabriela; de Souza, Marcia Denize Oliveira; Salvador, Mirian; Moura, Sidnei; Padilha, Francine Ferreira; Seixas, Fabiana Kömmling; Collares, Tiago; Borsuk, Sibele; Dellagostin, Odir Antonio; Henriques, João Antonio Pêgas; Roesch-Ely, Mariana

    2013-02-01

    Propolis is known for a long time for its health benefits and biological activities. Here, the red variety from the northeast of Brazil was chemically analyzed and extracts were investigated regarding their antioxidant and antitumor activity. Hydroalcoholic extracts, obtained from the red propolis, revealed polyphenol content, 2,2-diphenyl-1-picrylhydrazyl scavenging potential and enzymatic activities for catalase-like and superoxide dismutase-like. Cytotoxic activity was evaluated for human laryngeal epidermoid carcinoma cell (Hep-2), human cervical adenocarcinoma (HeLa) and human normal epithelial embryonic kidney (Hek-293). Survival analysis for non-tumor cell line showed greater IC50 compared to tumor cell lines, suggesting an increased sensitivity that may correlate with the higher proliferative index of the tumor vs. normal cells. Our results indicate that the Brazilian red propolis is capable of inhibiting cancer cell growth and constitutes an excellent source of antioxidant and antitumor natural agent. Copyright © 2012 Elsevier Ltd. All rights reserved.

  2. Phenolic content, antioxidant and antibacterial activity of selected natural sweeteners available on the Polish market.

    Science.gov (United States)

    Grabek-Lejko, Dorota; Tomczyk-Ulanowska, Kinga

    2013-01-01

    Seventeen natural sweeteners available on the Polish market were screened for total phenolic content, by the Folin-Ciocalteu method, and for antioxidant activity, using the ferric reducing antioxidant power (FRAP) assay and the 2,2'-Azinobis (3-ethylbenzthiazoline-6-sulphonic acid) radical cation decolorization assay (ABTS(·+)). In addition, we analyzed antibacterial activities against Staphylococcus aureus strains: both those susceptible and those resistant to methicillin (MRSA). The results of the study showed that total phenolic content, antioxidant activity and antibacterial activity differ widely among different samples of sweeteners. Phenolic content, expressed as a gallic acid equivalent, ranged from 0 mg kg(-1) in white, refined sugar, xylitol and wheat malt syrup to 11.4 g kg(-1) in sugarcane molasses. Antioxidant activity was lowest in refined white sugar, xylitol, brown beet sugar, liquid fructose, and rape honey; it was average in spelt syrup and corn syrup, and highest in sugar cane, beet molasses, date and barley syrups. Despite the great variety of sweeteners, a strong correlation was noted between the concentration of phenolics and antioxidant properties, as determined by the ABTS(·+) method (r = 0.97) and the FRAP assay (r = 0.77). The strongest antibacterial activity was observed in sugarcane molasses, which was lethal to S. aureus strains at 2 and 4% concentrations in medium for susceptible and MRSA strains respectively. Other sweeteners kill bacteria in 6-15% solutions, whereas some did not show any antibacterial activities against S. aureus strains, even at 20% concentrations. Due to their high antioxidant and antibacterial activities, some of the tested sweeteners have potential therapeutic value as supporting agents in antibiotic therapy.

  3. HIGH HYDROSTATIC PRESSURE EXTRACTION OF ANTIOXIDANTS FROM MORINDA CITRIFOLIA FRUIT – PROCESS PARAMETERS OPTIMIZATION

    Directory of Open Access Journals (Sweden)

    PRAVEEN KUMAR

    2006-06-01

    Full Text Available A modified version of high hydrostatic pressure extraction has been performed for extraction of antioxidants from M. citrifolia fruit at 5, 15, 25 bar and temperature 30° to 70°C for time duration 1, 2, 4 and 6 hours. The antioxidant activity of the extracts was determined by di-phenylpicrylhydrazyl radical scavenging method. The process parameters were optimized for antioxidant activity by central composite design method of response surface methodology using the statistical package, design expert. The results are expressed as 3D surface graphs. The optimum antioxidant activity was achieved at 58°C and 5 hours for 25bar. The optimal result achieved was within the region of response surface methodology. The statistical results were compared with the experimental result at 25bar, 2hour and 30° to 70°C and were found to be in proximate. The antioxidant activities of the extracts were found to increase with increase in pressure. It was also found that the response surface methodology works effectively for shorter range of parameters considered.

  4. Antioxidant and Antiplasmodial Activities of Bergenin and 11-O-Galloylbergenin Isolated from Mallotus philippensis.

    Science.gov (United States)

    Khan, Hamayun; Amin, Hazrat; Ullah, Asad; Saba, Sumbal; Rafique, Jamal; Khan, Khalid; Ahmad, Nasir; Badshah, Syed Lal

    2016-01-01

    Two important biologically active compounds were isolated from Mallotus philippensis. The isolated compounds were characterized using spectroanalytical techniques and found to be bergenin (1) and 11-O-galloylbergenin (2). The in vitro antioxidant and antiplasmodial activities of the isolated compounds were determined. For the antioxidant potential, three standard analytical protocols, namely, DPPH radical scavenging activity (RSA), reducing power assay (RPA), and total antioxidant capacity (TAC) assay, were adopted. The results showed that compound 2 was found to be more potent antioxidant as compared to 1. Fascinatingly, compound 2 displayed better EC50 results as compared to α-tocopherol while being comparable with ascorbic acid. The antiplasmodial assay data showed that both the compound exhibited good activity against chloroquine sensitive strain of Plasmodium falciparum (D10) and IC50 values were found to be less than 8 μM. The in silico molecular docking analyses were also performed for the determination of binding affinity of the isolated compounds using P. falciparum proteins PfLDH and Pfg27. The results showed that compound 2 has high docking score and binding affinity to both protein receptors as compared to compound 1. The demonstrated biological potentials declared that compound 2 could be the better natural antioxidant and antiplasmodial candidate.

  5. Antioxidant and Antiplasmodial Activities of Bergenin and 11-O-Galloylbergenin Isolated from Mallotus philippensis

    Directory of Open Access Journals (Sweden)

    Hamayun Khan

    2016-01-01

    Full Text Available Two important biologically active compounds were isolated from Mallotus philippensis. The isolated compounds were characterized using spectroanalytical techniques and found to be bergenin (1 and 11-O-galloylbergenin (2. The in vitro antioxidant and antiplasmodial activities of the isolated compounds were determined. For the antioxidant potential, three standard analytical protocols, namely, DPPH radical scavenging activity (RSA, reducing power assay (RPA, and total antioxidant capacity (TAC assay, were adopted. The results showed that compound 2 was found to be more potent antioxidant as compared to 1. Fascinatingly, compound 2 displayed better EC50 results as compared to α-tocopherol while being comparable with ascorbic acid. The antiplasmodial assay data showed that both the compound exhibited good activity against chloroquine sensitive strain of Plasmodium falciparum (D10 and IC50 values were found to be less than 8 μM. The in silico molecular docking analyses were also performed for the determination of binding affinity of the isolated compounds using P. falciparum proteins PfLDH and Pfg27. The results showed that compound 2 has high docking score and binding affinity to both protein receptors as compared to compound 1. The demonstrated biological potentials declared that compound 2 could be the better natural antioxidant and antiplasmodial candidate.

  6. Alkyl caffeates improve the antioxidant activity, antitumor property and oxidation stability of edible oil.

    Directory of Open Access Journals (Sweden)

    Jun Wang

    Full Text Available Caffeic acid (CA is distributed widely in nature and possesses strong antioxidant activity. However, CA has lower solubility in non-polar media, which limits its application in fat-soluble food. To increase the lipophilicity of natural antioxidant CA, a series of alkyl caffeates were synthesized and their antioxidant and antitumor activities were investigated. The antioxidant parameters, including the induction period, acid value and unsaturated fatty acid content, of the alkyl caffeates in edible oil were firstly investigated. The results indicated that alkyl caffeates had a lower DPPH IC₅₀ (14-23 µM compared to CA, dibutyl hydroxy toluene (BHT and Vitamin C (24-51 µM, and significantly inhibited four human cancer cells (SW620, SW480, SGC7901 and HepG2 with inhibition ratio of 71.4-78.0% by a MTT assay. With regard to the induction period and acid value assays, methyl and butyl caffeates had higher abilities than BHT to restrain the oxidation process and improve the stability of edible oil. The addition of ethyl caffeate to oil allowed maintenance of a higher unsaturated fatty acid methyl ester content (68.53% at high temperatures. Overall, the alkyl caffeats with short chain length (n<5 assessed better oxidative stability than those with long chain length. To date, this is the first report to the correlations among the antioxidant activity, anticancer activity and oxidative stability of alkyl caffeates.

  7. Evaluation of Antioxidant Activity and Acute Toxicity of Clausena excavata Leaves Extract

    Directory of Open Access Journals (Sweden)

    Shaymaa Fadhel Abbas Albaayit

    2014-01-01

    Full Text Available Clausena excavata (Lour., locally known as “Kemantu hitam,” is a common plant in Malaysian folklore medicine. This study evaluated the antioxidant properties of the solvent extracts of C. excavata leaves and determined the acute toxicity of methanolic extract C. excavata (MECE leaves in Sprague-Dawley rats. Harvested leaves were dried and subjected to solvent extraction using petroleum ether, chloroform, ethyl acetate and methanol in succession. The antioxidant activity of each extract was determined using the ferric-reducing antioxidant power (FRAP and 2,2-diphenyl-1-picryl dihydrazyl (DPPH radical scavenging activity. The total phenolic content (TPC and total flavonoids content (TFC were estimated by Folin-Ciocalteu and ethanolic aluminium chloride method, respectively. The chloroform extract was found to be highest in flavonoid content, while the methanolic extract showed the highest TPC and antioxidant activity. There was no mortality in rats treated with MECE leaves even at a high dose of 5000 mg/kg body weight. However, the MECE leaves produced mild to moderate pathological changes in the liver and kidneys, shown by mild degenerative changes and leucocyte infiltration. The extract did not affect the haematological parameters or relative weights of the liver or kidneys. Overall, the MECE leaves have potent antioxidant activity and are presumed safe to be used orally as health-promoting product at low to moderate doses.

  8. Antioxidant Activities and Chemical Constituents of Flavonoids from the Flower of Paeonia ostii

    Directory of Open Access Journals (Sweden)

    Huifang Zhang

    2016-12-01

    Full Text Available Paeonia ostii is a traditional medicinal plant popularly used in China. This study intended to evaluate the antioxidant properties and the chemical components of the flavonoid-rich extracts from the flowers of P. ostii. The results showed that the flavonoid-rich extracts from the flowers of P. ostii had strong scavenging capacities on 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate (ABTS, hydroxyls, superoxide anions, and 1,1-diphenyl-2-picrylhydrazyl (DPPH radicals in a dose-dependent manner. Five flavonoids, dihydrokaempferol (1, apigenin-7-O-β-d-glucoside (2, apigenin-7-O-β-d-neohesperidoside (3, kaempferol-7-O-β-d-glucopyranoside (4, and kaempferol-3-O-β-d-glucopyranosyl-7-O-β-d-glucopyranoside (5, were isolated from the flavonoid-rich extracts of the flowers of P. ostii. High-performance liquid chromatography (HPLC analysis revealed that compounds 3 and 4 were abundant in the P. ostii flower and in flavonoid-rich extracts. The main components of the flower of P. ostii are flavonoids. The high antioxidant activity of the flavonoid-rich extracts may be attributed to the high content of flavonoids. The five isolated flavonoids were the primary antioxidant ingredients, and may play important roles in the strong antioxidant activities of this flower. Based on the obtained results, the flower of P. ostii could be a potential source of natural antioxidants in food and pharmaceutical applications.

  9. ANTIBACTERIAL ACTIVITY, PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF STEM OF NICOTIANA TABACUM

    National Research Council Canada - National Science Library

    Y Sharma; D Dua; A Nagar; N S Srivastava

    2016-01-01

    .... The purpose of this present study is to investigate the antibacterial activity, phytochemical screening and the antioxidant activity of aqueous and alcoholic extracts of the stem of Nicotiana tabacum...

  10. Evaluation of the Antimicrobial and Antioxidant Activities of Origanum dictamnus Extracts before and after Encapsulation in Liposomes

    Directory of Open Access Journals (Sweden)

    John Tsaknis

    2007-05-01

    Full Text Available The antioxidant and antimicrobial activity of methanol or dichloromethane extracts of O. dictamnus, produced from wild and organic cultivated specimens, were determined. The Rancimat and malondialdehyde (MDA by HPLC methods were used to measure the antioxidant action, in comparison with that of the common commercial antioxidants butylated hydroxytoluene (BHT and α-tocopherol. The extracts that presented high antioxidant activity were encapsulated in liposomes and their antioxidant action was again estimated using differential scanning calorimetry (DSC. Thermaloxidative decomposition of the samples (pure liposomes and encapsulated extracts and the modification of the main transition temperature for the lipid mixture and the splitting of the calorimetric peak in the presence of the antioxidants were also studied by the DSC method. All extracts showed antioxidant and antimicrobial activities. Their action proved superior to α-tocopherol. The methanol extract of organic cultivated O. dictamnus (240 ppm showed higher activity than butylated hydroxytoluene. After encapsulation in liposomes the antioxidant as well as antimicrobial activities proved to be higher than those of the same extracts in pure form.

  11. Prevention of a systematic underestimation of antioxidant activity in competition assays. The impact of unspecific reactions of the reactive species.

    Science.gov (United States)

    Beljaars, Christiaan P; Balk, Jiska M; Bast, Aalt; Haenen, Guido R M M

    2010-02-12

    In antioxidant competition assays, an antioxidant (A) and a detector compound (D) compete for a reactive species (R). In the evaluation of these assays, it is tacitly assumed that all of R is captured by either D or A. Due to the - by definition - high reactivity of R, unspecific reactions of R are likely to occur and neglecting these reactions will result in a systematic underestimation of antioxidant activity. It was shown that in the standard hydroxyl radical scavenging assay this was indeed the case; the inaccurate mathematical evaluation resulted in an underestimation of antioxidant activity of 25% in this competition assay. The systematic underestimation of antioxidant activity can be prevented by using an adjusted Stern-Volmer equation that takes into account that only part of R is captured by D or A.

  12. Bioactive compounds and antioxidant activity of wolfberry infusion

    Science.gov (United States)

    Sun, Yujing; Rukeya, Japaer; Tao, Wenyang; Sun, Peilong; Ye, Xingqian

    2017-01-01

    An infusion of the wolfberry (Lycium barbarum L.) is a traditional Asian herbal tea. This is the most commonly consumed form of dried wolfberry worldwide, yet little scientific information on wolfberry infusions is available. We investigated the effects of making infusions with hot water on the color, the content of bioactive compounds (polysaccharides, polyphenols, flavonoids and carotenoids) and the antioxidant ability of wolfberry infusions. The contents of bioactive compounds and the antioxidant activity of a wolfberry infusion increased with increased infusion temperature and time. Total polysaccharides content (TPOC), total polyphenols (TPC), total flavonoids (TFC) and total carotenoids contents (TCC) were important for determining the antioxidant capacity of wolfberry infusions with the contribution to antioxidant activity in the order TPC > TFC > TCC > TPOC. Hierarchical cluster analysis indicated preparation conditions of 100 °C for 1~3 h, 90 °C for 2~3 h and 80 °C for 2.5~3 h were equivalent as regards the value of TPC, TPOC, TFC, TCC, FRAP, DPPH and ABTS. The results of this study suggest the length of time of making a wolfberry infusion in actual real life practice is too short and different dietary habits associated with the intake of wolfberry infusion might provide the same bioactive nutrients. PMID:28102295

  13. Trace elements as an activator of antioxidant enzymes

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    Marta Wołonciej

    2016-12-01

    Full Text Available Oxidative stress is a state of impaired balance between the formation of free radicals and antioxidant capacity of the body. It causes many defects of the body, e.g. lipid peroxidation, DNA and protein damage. In order to prevent the effects of oxidative stress, the organism has developed defence mechanisms. These mechanisms capture and inhibit the formation of free radicals and also chelate ion metals that catalyse free radical reactions. Trace elements are components of antioxidant enzymes involved in antioxidant mechanisms. Selenium, as a selenocysteine, is a component of the active site of glutathione peroxidase (GPx. The main function of GPx is neutralization of hydrogen peroxide (H2O2 and organic peroxide (LOOH. Furthermore, selenium is a structural part of a large group of selenoproteins that are necessary for proper functioning of the body. Manganese, copper and zinc are a part of the group of superoxide dismutase enzymes (MnSOD, Cu/ZnSOD, which catalyse the superoxide anion dismutation into hydrogen peroxide and oxygen. Formed hydrogen peroxide is decomposed into water and oxygen by catalase or glutathione peroxidase. An integral component of catalase (CAT is iron ions. The concentration of these trace elements has a significant influence on the activity of antioxidant enzymes, and thus on defence against oxidative stress. Even a small change in the level of trace elements in the tissue causes a disturbance in their metabolism, leading to the occurrence of many diseases.

  14. Screening of antidiabetic and antioxidant activities of medicinal plants

    Institute of Scientific and Technical Information of China (English)

    Amal Bakr Shori

    2015-01-01

    Diabetes is a common metabolic disorder characterized by abnormaly increased plasma glucose levels. Postprandial hyperglycemia plays an essential role in development of type-2 diabetes. Inhibitors of carbohydrate-hydrolyzing enzymes (such as α-glucosidase and α-amylase) offer an effective strategy to regulate/prevent hyperglycemia by controling starch breakdown. Natural α-amylase and α-glucosidase inhibitors, as wel as antioxidants from plant-based sources, offer a source of dietary ingredients that affect human physiological function in order to treat diabetes. Several research studies have investigated the effectiveness of plant-based inhibitors of α-amylase and α-glucosidase, as wel as their antioxidant activity. The aim of this review is to summarize the antidiabetic and antioxidant properties of several medicinal plants around the world. Half inhibitory concentration (IC50,for enzyme suppression) and half effective concentration (EC50, for antioxidant activity) values of less than 500 μg/mL were deifned as the most potent plant-based inhibitors (in vitro) and are expected to provide interesting candidates for herbal treatment of diabetes, as foods, supplements, or reifned drugs.

  15. Antioxidant and anti-atherogenic activities of olive oil phenolics.

    Science.gov (United States)

    Turner, Rufus; Etienne, Nicolas; Alonso, Maria Garcia; de Pascual-Teresa, Sonia; Minihane, Anne Marie; Weinberg, Peter D; Rimbach, Gerald

    2005-01-01

    The aim of the current study was to investigate the antioxidant and cellular activity of the olive oil phenolics oleuropein, tyrosol, hydroxytyrosol, and homovanillic alcohol (which is also a major metabolite of hydroxytyrosol). Well-characterized chemical and biochemical assays were used to assess the antioxidant potential of the compounds. Further experiments investigated their influence in cell culture on cytotoxic effects of hydrogen peroxide and oxidized low-density lipoprotein (LDL), nitric oxide production by activated macrophages, and secretion of chemoattractant and cell adhesion molecules by the endothelium. Inhibitory influences on in vitro platelet aggregation were also measured. The antioxidant assays indicated that homovanillic alcohol was a significantly more potent antioxidant than the other phenolics, both in chemical assays and in prolonging the lag phase of LDL oxidation. Cell culture experiments suggested that the olive oil phenolics induce a significant reduction in the secretion of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 (and a trend towards a reduced secretion of monocyte chemoattractant protein-1), and protect against cytotoxic effects of hydrogen peroxide and oxidized LDL. However, no influence on nitric oxide production or platelet aggregation was evident. The data show that olive oil phenolics have biochemical and cellular actions, which, if also apparent in vivo, could exert cardioprotective effects.

  16. Antioxidant and Anticancer Activities of Wampee (Clausena lansium (Lour. Skeels Peel

    Directory of Open Access Journals (Sweden)

    K. Nagendra Prasad

    2009-01-01

    Full Text Available Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT. In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901, human hepatocellular liver carcinoma (HepG-2 and human lung adenocarcinoma (A-549 cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

  17. Antioxidant activity of newly discovered lineage of marine actinobacteria.

    Science.gov (United States)

    Karthik, Loganathan; Kumar, Gaurav; Bhaskara Rao, Kokati Venkata

    2013-04-13

    To investigate the antioxidant activity of marine actinobacteria. The content of total phenolics, the level of antioxidant potential by DPPH radical scavenging activity, metal chelating activity, FRAP method, β carotene assay and NO scavenging activity in extract were determined. In all the methods the extract exhibited good scavenging activity except NO scavenging activity. The IC(50) values of marine actinobacteria extract on DPPH radical were found to be 41.09 μg/mL. The zone of color retention was 12 mm in β-carotene bleaching assay. DNA protective efficiency of the extracts was also studied using UV-photolysed H(2)O(2)-driven oxidative damage to pBR322. HPLC analysis identified some of the major phenolic compounds in extracts, which might be responsible for the antioxidant potential and cyto-protection. It showed a 100% cytotoxic effect in brine shrimp lethality assay within 10 mins. The novel actinobacteria was identified as Streptomyces LK-3 (JF710608) through 16S rDNA Sequencing. The results obtained suggest that the extracts bear anti-cancer metabolites and could be considered as a potential source for anti-cancer drug development. Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.

  18. Anti hypoxic and antioxidant activity of Hibiscus esculentus seeds

    Energy Technology Data Exchange (ETDEWEB)

    Ebrahimzadeh, M. A.; Nabavi, S. F.; Nabavi, S. M.; Eslami, B.

    2010-07-01

    The anti hypoxic and antioxidant activities of Hibiscus esculentus seeds were investigated employing eight in vitro assay systems. Anti hypoxic activity was investigated in two models, haemic and circulatory. The effects were pronounced in both models of hypoxia. The anti hypoxic effects were dose-dependent. The results indicated that the extracts have a protective effect against hypoxia induced lethality in mice. The extracts showed antioxidant activity in some models. IC{sub 5}0 for DPPH radical-scavenging activity was 234 {+-} 8.9 {mu}g ml{sup 1}. The extracts showed weak nitric oxide-scavenging activity between 0.1 and 1.6 mg ml{sup -}1. The extracts showed weak Fe{sup 2}+ chelating ability. IC{sub 5}0 were 150 {+-} 13 {mu}g ml{sup -}1. The extracts also exhibited low antioxidant activity in the linoleic acid model but were capable of scavenging hydrogen peroxide in a concentration dependent manner. The total amount of phenolic compounds in each extract was determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Pharmacological effects may be attributed, at least in part, to the presence of phenols and flavonoids in the extracts. (Author) 40 refs.

  19. Antimicrobial and antioxidant activity of natural honeys of different origin

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    Miartina Fikselová

    2014-11-01

    Full Text Available To examine the antimicrobial and antioxidant activity of 15 natural honeys, honey samples were collected from different locations of Slovakia, Poland and Serbia. For antimicrobial activity determination honey solutions were prepared at three concentrations: 50, 25 and 12.5 % (by mass per volume. The potential antimicrobial activity of  selected samples against four species of bacteria (Escherichia coli CCM 3988, Pseudomonas aeroginosa CCM 1960, Staphylococcus epidermis CCM 4418, Bacillus cereus CCM 2010 and two species of yeasts (Saccharomyces cerevisiae CCM 8191, Candida albicans CCM 8216 was studied using the disc diffusion method. After incubation, the zones of inhibition of the growth of the microorganisms around the disks were measured. The strongest antimicrobial activity was shown at honey samples of 50 % concentration against Escherichia coli, Pseudomonas aeroginosa and Staphylococcus epidermis. Against Saccharomyces cerevisae and Candida albicans very low (at 50 %, 25 % concentration or zero antifugal (at 12.5 % concentration activity was determined. From the results obtained it was shown the variable ability of honey samples to scavenge stable free radical DPPH. TEACDPPH values ranged between 0.1-1.0 mmol.kg-1. As the antioxidative best source buckwheat honey was manifested and the lowest antioxidant activity was shown at acacia honey.

  20. Increased Antioxidant Quality Versus Lower Quantity Of High Density Lipoprotein In Benign Prostatic Hyperplasia

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    Aydin Ozgur

    2015-10-01

    Full Text Available Background: Oxidative stress may be involved in the pathogenesis of every human disease. To understand its possible role in benign prostatic hyperplasia (BPH, we measured the overall oxidative status of patients with BPH and the serum activity of the high density lipoprotein (HDL-related antioxidant enzymes paraoxonase 1 (PON1 and arylesterase (ARE.

  1. Bioprotective properties of seaweeds: In vitro evaluation of antioxidant activity and antimicrobial activity against food borne bacteria in relation to polyphenolic content

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    Kesika Periyanaina

    2008-07-01

    Full Text Available Abstract Background For many years chemical preservatives have been used in food, to act as either antimicrobials or antioxidants or both. In general, consumers regard additive-free foods as safer since preservatives can cause health hazards like asthma and cancer and are suspected to be mutagenic and neurotoxic. The present study was carried out to evaluate the antimicrobial and antioxidant activity of methanolic extracts of seaweeds, with a view to developing safer food preservatives. Methods Ten edible seaweeds, which have wide pharmaceutical application, were collected from Central Marine Fisheries Research Institute, Tamil Nadu, India and evaluated for antioxidant and antimicrobial activity against food borne pathogens. Results The results indicate that Gelidiella acerosa has the highest antioxidant activity while Haligra sps exhibited antibacterial activity against Staphylococcus aureus (MTCC 96. Conclusion Quantitative analysis of the total phenolic content of the seaweeds indicated that Gelidella acerosa and Haligra sps have high phenolic contents, which correlated to their respective antioxidant and antimicrobial activity

  2. Bioprotective properties of seaweeds: in vitro evaluation of antioxidant activity and antimicrobial activity against food borne bacteria in relation to polyphenolic content.

    Science.gov (United States)

    Devi, Kasi Pandima; Suganthy, Natarajan; Kesika, Periyanaina; Pandian, Shanmugaiahthevar Karutha

    2008-07-10

    For many years chemical preservatives have been used in food, to act as either antimicrobials or antioxidants or both. In general, consumers regard additive-free foods as safer since preservatives can cause health hazards like asthma and cancer and are suspected to be mutagenic and neurotoxic. The present study was carried out to evaluate the antimicrobial and antioxidant activity of methanolic extracts of seaweeds, with a view to developing safer food preservatives. Ten edible seaweeds, which have wide pharmaceutical application, were collected from Central Marine Fisheries Research Institute, Tamil Nadu, India and evaluated for antioxidant and antimicrobial activity against food borne pathogens. The results indicate that Gelidiella acerosa has the highest antioxidant activity while Haligra sps exhibited antibacterial activity against Staphylococcus aureus (MTCC 96). Quantitative analysis of the total phenolic content of the seaweeds indicated that Gelidella acerosa and Haligra sps have high phenolic contents, which correlated to their respective antioxidant and antimicrobial activity.

  3. The dual effects of Maillard reaction and enzymatic hydrolysis on the antioxidant activity of milk proteins.

    Science.gov (United States)

    Oh, N S; Lee, H A; Lee, J Y; Joung, J Y; Lee, K B; Kim, Y; Lee, K W; Kim, S H

    2013-08-01

    The objective of this study was to determine the enhanced effects on the biological characteristics and antioxidant activity of milk proteins by the combination of the Maillard reaction and enzymatic hydrolysis. Maillard reaction products were obtained from milk protein preparations, such as whey protein concentrates and sodium caseinate with lactose, by heating at 55°C for 7 d in sodium phosphate buffer (pH 7.4). The Maillard reaction products, along with untreated milk proteins as controls, were hydrolyzed for 0 to 3h with commercial proteases Alcalase, Neutrase, Protamex, and Flavorzyme (Novozymes, Bagsværd, Denmark). The antioxidant activity of hydrolyzed Maillard reaction products was determined by reaction with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, their 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, and the ability to reduce ferric ions. Further characteristics were evaluated by the o-phthaldialdehyde method and sodium dodecyl sulfate-PAGE. The degree of hydrolysis gradually increased in a time-dependent manner, with the Alcalase-treated Maillard reaction products being the most highly hydrolyzed. Radical scavenging activities and reducing ability of hydrolyzed Maillard reaction products increased with increasing hydrolysis time. The combined products of enzymatic hydrolysis and Maillard reaction showed significantly greater antioxidant activity than did hydrolysates or Maillard reaction products alone. The hydrolyzed Maillard reaction products generated by Alcalase showed significantly higher antioxidant activity when compared with the other protease products and the antioxidant activity was higher for the whey protein concentrate groups than for the sodium caseinate groups. These findings indicate that Maillard reaction products, coupled with enzymatic hydrolysis, could act as potential antioxidants in the pharmaceutical, food, and dairy industries.

  4. Anticandidal, antibacterial, cytotoxic and antioxidant activities of Calendula arvensis flowers.

    Science.gov (United States)

    Abudunia, A-M; Marmouzi, I; Faouzi, M E A; Ramli, Y; Taoufik, J; El Madani, N; Essassi, E M; Salama, A; Khedid, K; Ansar, M; Ibrahimi, A

    2016-12-20

    Calendula arvensis (CA) is one of the important plants used in traditional medicine in Morocco, due to its interesting chemical composition. The present study aimed to determine the anticandidal, antioxidant and antibacterial activities, and the effects of extracts of CA flowers on the growth of myeloid cancer cells. Also, to characterize the chemical composition of the plant. Flowers of CA were collected based on ethnopharmacological information from the villages around the region Rabat-Khemisset, Moroccco. The hexane and methanol extracts were obtained by soxhlet extraction, while aqueous extracts was obtained by maceration in cold water. CA extracts were assessed for antioxidant activity using four different methods (DPPH, FRAP, TEAC, β-carotene bleaching test). Furthermore, the phenolic and flavonoid contents were measured, also the antimicrobial activity has been evaluated by the well diffusion method using several bacterial and fungal strains. Finally, extracts cytotoxicity was assessed using MTT test. Phytochemical quantification of the methanolic and aqueous extracts revealed that they were rich with flavonoid and phenolic content and were found to possess considerable antioxidant activities. MIC values of methanolic extracts were 12.5-25μg/mL. While MIC values of hexanolic extracts were between 6.25-12.5μg/mL and were bacteriostatic for all bacteria while methanolic and aqueous extracts were bactericidal. In addition, the extracts exhibited no activity on Candida species except the methanolic extract, which showed antifungal activity onCandida tropicalis 1 and Candida famata 1. The methanolic and aqueous extracts also exhibited antimyeloid cancer activity (IC50 of 31μg/mL). In our study, we conclude that the methanolic and aqueous extracts were a promising source of antioxidant, antimicrobial and cytotoxic agents.

  5. Total Content of Polyphenols and Antioxidant Activity of Different Melliferous Plants

    OpenAIRE

    2016-01-01

    In this study polyphenols content and antioxidant activity of melliferous plants for the following: mint (Mentha pulegium), burdock (Arctium lappa), comfrey (Symphytum officinale), plantain (Pantago lanceolata), thyme (Thymus vulgaris), sage (Salvia officinalis), marigold (Calendula officinalis), small marshmallow (Althaea officinalis), echinacea (Echinaceea angustifolia) and black popular (Populus nigra) were investigated, using two different extraction methods. High content of polyp...

  6. Determination of antioxidant activity of spices and their active principles by differential pulse voltammetry.

    Science.gov (United States)

    Palma, Alberto; Ruiz Montoya, Mercedes; Arteaga, Jesús F; Rodríguez Mellado, Jose M

    2014-01-22

    The anodic oxidation of mercury in the presence of hydrogen peroxide in differential pulse voltammetry (DPV) was used to determine the antioxidant (AO) character of radical scavengers. Hydroperoxide radical is formed at the potentials of the oxidation peak on mercury electrodes, such radical reacting with the antioxidants in different extension. The parameter C10 (antioxidant concentration at which the peak area decreases by 10%) is used to measure the scavenging activity of the individual antioxidants. To establish the scavenging activity of antioxidant mixtures as a whole, the parameter, μ10 as the reverse of V10, V10 being the volume necessary to decrease the peak area in DPV by 10%, was selected. Higher μ10 values correspond to higher scavenging activity. The studies have been extended to aqueous extracts of some species. The results may be useful in explaining the effect of spices in vitro and in vivo studies.

  7. Polysaccharides of higher fungi: Biological role, structure, and antioxidative activity

    Directory of Open Access Journals (Sweden)

    Kozarski Maja S.

    2014-01-01

    Full Text Available Fungal polysaccharides attract a lot of attention due to their multiple challenging biological properties, such as: anti-tumor, anti-viral, anticomplementary, anticoagulant, hypolipidemic and immunomodulatory and immune-stimulatory activities, which all together make them suitable for application in many quite distinctive areas, such as food industry, biomedicine, cosmetology, agriculture, environmental protection and waste water management. This article presents results with respect to biological properties, structure and procedures related to the isolation and activation of polysaccharides of higher fungi. It is considered and presented along with a review of the critical antioxidative activity and possible influence of the structural composition of polysaccharide extracts (isolated from these higher fungi upon their antioxidative properties.

  8. Antibacterial and antioxidant activities of Licania tomentosa (Benth. fritsch (crhysobalanaceae

    Directory of Open Access Journals (Sweden)

    Silva J.B.N.F.

    2012-01-01

    Full Text Available This work describes the chemical composition, and evaluates the antimicrobial and antioxidant activities of a hydroalcoholic extract from the leaves of the Licania tomentosa. Gram positive and negative bacterial strains were used in this work. Examination of the phytochemical composition of L. tomentosa revealed the presence of secondary metabolites such as tannins, flavonoids, saponins, alkaloids, steroids and triterpenoids. An antibacterial assay pointed out that the extract had a lower minimal inhibitory concentration (MIC - 32 μg/mL towards Staphylococcus aureus (ATCC12692. The extract also presented antibacterial activity against other assayed bacteria, with the MIC varying between 64 and 512 μg/ mL. Our findings reveal that the extract presented an antioxidative capacity lower than that of BHT at the same concentration, used as positive control. Our results suggest that the levels and combinations between the secondary metabolites of this plant should be investigated to explain the demonstrated antibacterial activity.

  9. Antioxidant activity of noni juice in heavy smokers

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    Wang Mian-Ying

    2009-10-01

    Full Text Available Abstract Background Noni (Morinda citrifolia juice has demonstrated antioxidant activity in vitro and in vivo. To evaluate this activity in humans, noni juice from Tahiti (TNJ was evaluated in a 30 day, double-blind, and placebo controlled clinical trial with 285 current heavy smokers. Research participants were randomly assigned to three daily treatment groups: 118 mL placebo, 29.5 mL TNJ, and 118 mL TNJ. Plasma superoxide anion radicals (SAR and lipid hydroperoxide (LOOH levels were measured pre and post-intervention. Results After 30 days, mean SAR decreased from 0.26 ± 0.14 to 0.19 ± 0.10 μmol/mL in the 29.5 mL dose group (P P P P Conclusion The results suggest an antioxidant activity from noni juice in humans exposed to tobacco smoke, thereby replicating the results found previous chemical and in vivo tests.

  10. [Antioxidant and antibacterial activities of dimeric phenol compounds].

    Science.gov (United States)

    Ogata, Masahiro

    2008-08-01

    We studied the antioxidant and antibacterial activities of monomeric and dimeric phenol compounds. Dimeric compounds had higher antioxidant activities than monomeric compounds. Electron spin resonance spin-trapping experiments showed that phenol compounds with an allyl substituent on their aromatic rings directly scavenged superoxide, and that only eugenol trapped hydroxyl radicals. We developed a generation system of the hydroxyl radical without using any metals by adding L-DOPA and DMPO to PBS or MiliQ water in vitro. We found that eugenol trapped hydroxyl radicals directly and is metabolized to a dimer. On the other hand, dipropofol, a dimer of propofol, has strong antibacterial activity against Gram-positive bacteria. However, it lacks solubility in water and this property is assumed to limit its efficacy. We tried to improve the solubility and found a new solubilization method of dipropofol in water with the addition of a monosaccharide or ascorbic acid.

  11. Antioxidant activity in barley (Hordeum Vulgare L.) grains roasted in a microwave oven under conditions optimized using response surface methodology.

    Science.gov (United States)

    Omwamba, Mary; Hu, Qiuhui

    2010-01-01

    Microwave processing and cooking of foods is a recent development that is gaining momentum in household as well as large-scale food applications. Barley contains phenol compounds which possess antioxidant activity. In this study the microwave oven roasting condition was optimized to obtain grains with high antioxidant activity measured as the ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. Antioxidant activity of grains roasted under optimum conditions was assessed based on DPPH radical scavenging activity, reducing power and inhibition of oxidation in linoleic acid system. The optimum condition for obtaining roasted barley with high antioxidant activity (90.5% DPPH inhibition) was found to be at 600 W microwave power, 8.5 min roasting time, and 61.5 g or 2 layers of grains. The roasting condition influenced antioxidant activity both individually and interactively. Statistical analysis showed that the model was significant (P < 0.0001). The acetone extract had significantly high inhibition of lipid peroxidation and DPPH radical scavenging activity compared to the aqueous extract and alpha-tocopherol. The reducing power of acetone extracts was not significantly different from alpha-tocopherol. The acetone extract had twice the amount of phenol content compared to the aqueous extract indicating its high extraction efficiency. GC-MS analysis revealed the presence of phenol acids, amino phenols, and quinones. The aqueous extract did not contain 3,4-dihydroxybenzaldehyde and 4-hydroxycinnamic acid which are phenol compounds reported to contribute to antioxidant activity in barley grain.

  12. Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds

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    Diaz Patricia

    2012-11-01

    Full Text Available Abstract Background This study aims to determine the relationship between the antioxidant and anti-inflammatory activities of the thirteen herbs and two fungi extracts, and their total phenolic and flavonoid contents. Methods Antioxidant activities were evaluated by four assays: an antioxidant activity assay using Saccharomyces cerevisiae, a DPPH ((2, 2-diphenyl-1-picrylhydrazyl assay to assess free radical scavenging, an assay assessing ferrous ions or iron (II chelating ability, and a ferric reducing antioxidant power (FRAP assay. Total phenolic and flavonoid contents were determined using the Folin-Ciocalteu and aluminium chloride methods, respectively. Anti-inflammatory activities were determined by measuring the inhibition of nitric oxide and TNF-α production in lipopolysaccharide- and interferon-γ-activated J774A.1 macrophages. Their cytotoxicities against macrophages were determined by MTT assay. Results A positive linear correlation between antioxidant activities and the total phenolic and flavonoid content of the plant extracts was found. The plant extracts with high phenolic and flavonoid content also exhibited significant anti-inflammatory activity with good cell viability. Conclusion The selected herbs could be a rich source of antioxidants and free radical scavenging compounds. The levels of phenolic and flavonoid compounds were correlated with the antioxidant and anti-inflammatory activities of the extracts from the herbs.

  13. The antioxidant and cytotoxic activities of Sonchus oleraceus L. extracts.

    Science.gov (United States)

    Yin, Jie; Kwon, Gu-Joong; Wang, Myeong-Hyeon

    2007-01-01

    This study investigated in vitro antioxidant activity of Sonchus oleraceus L. by extraction solvent, which were examined by reducing power, hydroxyl radical-scavenging activity(HRSA) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays. 70% MeOH extract had the greatest reducing power while EtOH extract had the greatest HRSA. The antioxidant activity of S. oleraceus extracts was concentration dependent and its IC(50) values ranged from 47.1 to 210.5 microg/ml and IC(50) of 70% MeOH, boiling water and 70% EtOH extracts were 47.1, 52.7 and 56.5 microg/ml, respectively. 70% MeOH extract of S. oleraceus contained the greatest amount of both phenolic and flavonoid contents. The extracts tested had greater nitrite scavenging effects at lower pH conditions. The cytotoxic activity showed that EtOH extract had the best activity against the growth of stomach cancer cell. These results suggest that S. oleraceus extract could be used as a potential source of natural antioxidants.

  14. Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts

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    Chaabane Fadwa

    2012-09-01

    Full Text Available Abstract Background Plants play a significant role in maintaining human health and improving the quality of human life. They serve humans well as valuable components of food, as well as in cosmetics, dyes, and medicines. In fact, many plant extracts prepared from plants have been shown to exert biological activity in vitro and in vivo. The present study explored antioxidant and antigenotoxic effects of Daphne gnidium leaf extracts. Methods The genotoxic potential of petroleum ether, chloroform, ethyl acetate, methanol and total oligomer flavonoid (TOF enriched extracts from leaves of Daphne gnidium, was assessed using Escherichia coli PQ37. Likewise, the antigenotoxicity of the same extracts was tested using the “SOS chromotest test”. Antioxidant activities were studied using non enzymatic and enzymatic method: NBT/Riboflavine and xantine oxidase. Results None of the different extracts produced a genotoxic effect, except TOF extract at the lowest tested dose. Our results showed that D. gnidium leaf extracts possess an antigenotoxic effect against the nitrofurantoin a mutagen of reference. Ethyl acetate and TOF extracts were the most effective in inhibiting xanthine oxidase activity. While, methanol extract was the most potent superoxide scavenger when tested with the NBT/Riboflavine assay. Conclusions The present study has demonstrated that D. gnidium leaf extract possess antioxidant and antigenotoxic effects. These activities could be ascribed to compounds like polyphenols and flavonoid. Further studies are required to isolate the active molecules.

  15. Antioxidant and antimutagenic activity of Curcuma caesia Roxb. rhizome extracts

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    Heisanam Pushparani Devi

    2015-01-01

    Full Text Available The rhizomes of Curcuma caesia Roxb. (zingiberacea are traditionally used in treatment of various ailments and metabolic disorders like leukoderma, asthma, tumours, piles, bronchitis, etc. in Indian system of medicine. Considering the importance of natural products in modern phytomedicine, the antioxidant and antimutagenic activities of C. caesia Roxb. rhizome extract and its fractions were evaluated. The ethanolic fraction showed highest antioxidant activity by DPPH assay (86.91% comparable to ascorbic acid (94.77% with IC50 value of 418 μg/ml for EECC followed by MECC (441.90 μg/ml > EAECC(561 μg/ml > AECC(591 μg/ml. Based on the antioxidant activity, three of the rhizome extracts were evaluated for their antimutagenic properties against indirect acting mutagen cyclophosphamide (CP using Salmonella typhimurium strains TA98 and TA100. The antimutagenic activity of the extracts against indirect acting mutagen cyclophosphamide in the presence of mammalian metabolic activation system was found to be significant (p < 0.01, p < 0.05. All the extracts showed similar antimutagenicity in dose dependent manner. The total phenolic content as well as reducing ability of the extracts was also determined.