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Sample records for hidradenitis suppurativa hs

  1. Intralesional triamcinolone for flares of hidradenitis suppurativa (HS)

    DEFF Research Database (Denmark)

    Riis, Peter Theut; Boer, Jurr; Prens, Errol P

    2016-01-01

    BACKGROUND: Hidradenitis suppurativa (HS) is a chronic inflammatory disease of the hair follicle. Standard practice of managing acute flares with corticosteroid injection lacks scientific evidence. OBJECTIVE: We sought to assess the outcomes of routine treatment using intralesional triamcinolone...

  2. Hidradenitis suppurativa (HS) is associated with low socioeconomic status (SES) : A cross-sectional reference study

    NARCIS (Netherlands)

    Deckers, Inge E.; Janse, Ineke C.; van der Zee, Hessel H.; Nijsten, Tamar; Boer, Jurr; Horvath, Barbara; Prens, Errol P.

    2016-01-01

    Background: Hidradenitis suppurativa (HS) is a chronic debilitating disease, whereby school attendance and employment can be disturbed. Objective: We sought to determine the socioeconomic status (SES) in patients with HS relative to other dermatologic patients, and whether specific clinical HS

  3. Hidradenitis suppurativa.

    NARCIS (Netherlands)

    Buimer, M.G.; Wobbes, T.; Klinkenbijl, J.H.G.

    2009-01-01

    BACKGROUND: Hidradenitis suppurativa is a chronic, recurrent, suppurative cutaneous disease. Despite its incidence, optimal medical or surgical treatment remains unclear. This review describes the disease, ranging from pathogenesis to treatment and prognosis. METHODS: Articles were sourced from

  4. HiSCR (Hidradenitis Suppurativa Clinical Response)

    DEFF Research Database (Denmark)

    Kimball, B. A.; Sobell, J. M.; Zouboulis, C C

    2016-01-01

    Background: Determining treatment response for patients with hidradenitis suppurativa (HS) can be challenging due to limitations of current disease activity evaluations. Objective: Evaluate the novel, validated endpoint, Hidradenitis Suppurativa Clinical Response (HiSCR) and its utility as an out...

  5. Hidradenitis Suppurativa

    Directory of Open Access Journals (Sweden)

    Müzeyyen Gönül

    2009-03-01

    Full Text Available Hidradenitis suppurativa is a chronic inflammatory disorder of apocrine glands characterized by recurrent abscess, sinuses and scars. The disorder affects life quality significantly and usually involves axillae, groins, perineal and perianal regions. The etiology of the disease has been explained exactly. However follicular occlusion is thought to be the main cause. The treatment of the disorder is very difficult. The medical treatment is preferred for mild and modarate disease, whereas surgical treatment is suggested for severe disease. Among the medical treatment, there are systemic and topical antibiotics, intralesional steroid injection, systemic retinoids and hormonal therapy. The options of surgical treatment of disease change from drainage to radical excision, and the therapy should be choosen according to the severity of the disease. Whatever the therapeutic options are, recurrence is the most important causes of the morbidity of hidradenitis suppurativa.

  6. Coagulation Status in Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Miller, Iben Marie; Johansen, Maria Egede; Mogensen, Ulla B

    2015-01-01

    BACKGROUND: Chronic inflammatory diseases other than hidradenitis suppurativa (HS) have been associated with prothrombotic/hypercoagulable status. OBJECTIVE: To investigate a possible association between the chronic inflammatory skin disease HS and prothrombotic/hypercoagulable state. METHODS: We...

  7. Hidradenitis Suppurativa/Acne Inversa

    DEFF Research Database (Denmark)

    Zouboulis, Christos C.; Del Marmol, Véronique; Mrowietz, Ulrich

    2015-01-01

    BACKGROUND: Hidradenitis suppurativa/acne inversa (HS) is a chronic, inflammatory, recurrent, debilitating disease, which inflicts a significant burden on patients and is associated with comorbid disorders, such as significantly reduced quality of life, depression, stigmatization, inactivity...

  8. The bacteriology of hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Ring, Hans Christian; Riis Mikkelsen, Peter; Miller, Iben Marie

    2015-01-01

    Hidradenitis suppurativa (HS) is a chronic inflammatory disabling skin disease consisting of recurrent nodules, sinuses, fistulas and scarring involving the intertriginous regions. HS is often a therapeutic challenge and most treatments are off-label. A better understanding of aetiology......, supports a microbial role in disease pathogenesis. However, these antibiotics also work as immunomodulators of especially T cells, and the underlying mechanisms may therefore be more complex. We performed a systematic review of previous studies investigating the bacterial flora in hidradenitis suppurativa...

  9. Comorbidities of hidradenitis suppurativa.

    Science.gov (United States)

    Porter, Martina L; Kimball, Alexa B

    2017-06-01

    Hidradenitis suppurativa (HS) is an inflammatory skin disorder with many associated comorbidities, including obesity, metabolic syndrome, smoking, depression, arthritis, autoinflammatory syndromes, inflammatory bowel disease, and genetic syndromes. In addition, HS patients can suffer from a variety of diseases related to the chronic inflammatory nature of their HS such as cardiovascular disease and anemia. An understanding of these comorbidities and associations is essential for the management of HS, and routine screening for these entities should be considered in all HS patients. ©2017 Frontline Medical Communications.

  10. Dapsone therapy for hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Yazdanyar, S.; Jemec, G.B.E.; Boer, J.

    2011-01-01

    Background: Hidradenitis suppurativa (HS) is an inflammatory skin disease with a chronic intermittent course. HS is difficult to treat, and the evidence for the effect of most treatments consists of smaller open studies. The use of dapsone in the treatment of HS is based on a few published cases...

  11. Prevalence of Hidradenitis Suppurativa (HS): A Population-Based Study in Olmsted County, Minnesota

    Science.gov (United States)

    Shahi, Varun; Alikhan, Ali; Vazquez, Benjamin G.; Weaver, Amy L.; Davis, Mark D.

    2014-01-01

    BACKGROUND/AIMS Hidradenitis suppurativa (HS) is a follicular occlusion disorder occurring in apocrine-rich regions of the skin. Estimates of the prevalence of this disorder have not been population-based. We sought to provide population-based information on the prevalence of HS in Olmsted County, Minnesota as of 1/1/2009. METHODS Rochester Epidemiology Project, a unique infrastructure that combines and makes accessible all medical records in Olmsted County since the 1960s, was used to collect population-based data on the prevalence of HS. RESULTS We identified 178 confirmed cases of HS that included 135 females and 43 males, and estimated the total sex- and age-adjusted prevalence in Olmsted County to be 127.8 per 100,000 or 0.13%. The total prevalence was significantly higher among women than men. CONCLUSION This study represents the first population-based investigation on the prevalence of HS. In this population-based cohort, HS was less prevalent than previous reports have suggested. PMID:25228133

  12. Hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Dufour, Deirdre Nathalie; Emtestam, Lennart; Jemec, Gregor B

    2014-01-01

    pain, work disability and overall poor quality of life. Although the clinical signs of the disease can often be hidden by clothing, active HS is associated with a malodorous discharge that contributes to the disabling social stigma. Risk factors include smoking and obesity. Comorbidities include...

  13. Treatments for hidradenitis suppurativa.

    Science.gov (United States)

    Andersen, R Kjærsgaard; Jemec, Gregor B E

    Hidradenitis suppurativa (HS) is not easily treated. Although not uncommon, HS is often misdiagnosed outside specialized clinics and inappropriately treated as a simple boil or abscess. In recent years, guidelines have been developed on the basis of expert opinion and the available literature. A multifaceted approach is necessary as HS lesions include both inflammation (amenable to medical treatment) as well as fibrosis (amenable to surgery only). The recommended antiinflammatory therapies encompass both antimicrobials and regular anti-inflammatory drugs. We have, therefore, reviewed treatments with the following agents: clindamycin, tetracycline, rifampicin, ertapenem, dapsone, triamcinolone, infliximab, adalimumab, and anakinra. The development of new medical treatments, however, is an ongoing effort, and important new data have been presented since the publication of the guideline. The current approach to the management of fibrotic lesions is surgery. It is important, as manifest fibrosis is generally not susceptible to medical treatment. Here minor excision, carbon dioxide-laser, and major surgery are discussed, and current evidence supporting their use is provided. A comprehensive three-pronged approach with adjuvant therapy, medical therapy, and surgery is recommended. The importance of adjuvant therapy, that is, pain management, wound care, and attention, is stressed. Adjuvant therapy not only plays a major role in patients' perception of a successful treatment but also is of practical importance to their coping and self-management. HS presents a significant unmet need, and this review provides a mechanistic update on the current real-world therapeutic option for the management of this distressing disease.

  14. The Microbiology of Hidradenitis Suppurativa.

    Science.gov (United States)

    Ring, Hans Christian; Emtestam, Lennart

    2016-01-01

    Although the clinical presentation of Hidradenitis Suppurativa (HS) is strongly reminiscent of bacterial infection, the role of bacteria remains controversial. Studies have isolated an array of different bacterial specimens as well as biofilm formation in lesional HS skin. Consistent findings of Gram-positive cocci and -rods including Staphylococus aureus, Coagulase-negative staphylococci (CoNS) and Corynebacterium species (spp) in deep tissue samples have been demonstrated in HS. Although efficacy of antibiotics, i.e., rifampicin, clindamycin or tetracycline may support a microbial role in disease pathogenesis, the most often isolated bacterial specimens are commensal bacteria (CoNS).

  15. Pain management in patients with hidradenitis suppurativa

    NARCIS (Netherlands)

    Horvath, Barbara; Janse, Ineke C.; Sibbald, Gary R.

    2015-01-01

    Hidradenitis suppurativa (HS) is a chronic, relapsing, and painful inflammatory disease. HS patients' quality of life is severely impaired, and this impairment correlates strongly with their pain. Pain in HS can be acute or chronic and has both inflammatory and noninflammatory origins. The purpose o

  16. Pain management in patients with hidradenitis suppurativa

    NARCIS (Netherlands)

    Horvath, Barbara; Janse, Ineke C.; Sibbald, Gary R.

    2015-01-01

    Hidradenitis suppurativa (HS) is a chronic, relapsing, and painful inflammatory disease. HS patients' quality of life is severely impaired, and this impairment correlates strongly with their pain. Pain in HS can be acute or chronic and has both inflammatory and noninflammatory origins. The purpose

  17. Adalimumab for the treatment of moderate to severe Hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Kimball, Alexa B; Kerdel, Francisco; Adams, David

    2012-01-01

    Hidradenitis suppurativa (HS) is a chronic, painful skin disease characterized by abscesses, nodules, and draining fistulas in the axilla and groin of young adults.......Hidradenitis suppurativa (HS) is a chronic, painful skin disease characterized by abscesses, nodules, and draining fistulas in the axilla and groin of young adults....

  18. Hidradenitis suppurativa successfully treated with adalimumab

    Directory of Open Access Journals (Sweden)

    Iqbal A. Bukhari

    2015-10-01

    Full Text Available Aim: To discuss the new beneficial effect of adalimumab in the management of hidradenitis suppurativa(HS.Case: We report a 25-year-old arabic female with a 14-year history with long-standing poorly controlled active hidradenitis suppurativa who was successfully treated with adalimumab.Discussion: Hidradenitis suppurativa is a skin disorder characterized by the formation of multiple cysts, abscesses and sinus tracts in apocrine gland-bearing areas. The aetiology and pathogenesis of HS are unknown. Current medical and surgical therapies are only minimally effective at treating the disease. The biologic agent Adalimumab is a new promising agent for the treatment of HS.Conclusion: The biologic agent adalimumab is an effective treatment for HS

  19. Cyclosporine treatment of severe Hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Anderson, Marianne D; Zauli, Stefania; Bettoli, Vincenzo

    2016-01-01

    Background: Hidradenitis suppurativa (HS) is an overlooked but common disease severely affecting both genders. HS is generally perceived as difficult to treat and although a number of treatments are available, the need for more effective treatment is apparent. Objectives: Cyclosporine A (CsA) has...

  20. Hidradenitis Suppurativa in Children and Adolescents

    DEFF Research Database (Denmark)

    Mikkelsen, Peter Riis; Jemec, Gregor B E

    2014-01-01

    Hidradenitis suppurativa (HS) is a burdensome disease and has the potential to affect the life course of patients. It is a rare disease in children, and the recorded literature is correspondingly scarce. This article reviews the therapeutic options for HS in children and adolescents, and highlights...

  1. New treatment strategies for hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Andersen, R.; Jemec, G. B.E.

    2016-01-01

    Hidradenitis suppurativa (HS) is difficult to treat. Official guidelines have only recently been developed, and suggest that patients should be provided with both adjuvant, medical and surgical therapy. The guidelines are the result of resurgent interest in this disease, in which etiology....... In this article recent trends in medical treatments are described following a systematic review. Searches were conducted in MEDLINE®, the Cochrane Library and Web of ScienceTM where specific treatments were combined with the search term "hidradenitis suppurativa" and references from 2010 to March 2016 were...

  2. Depression in patients with hidradenitis suppurativa

    NARCIS (Netherlands)

    Onderdijk, A. J.; van der Zee, H. H.; Esmann, S.; Lophaven, S.; Dufour, D. N.; Jemec, G. B. E.; Boer, J.

    Background Hidradenitis suppurativa (HS) is a chronic recurrent inflammatory skin disease with abscess formation and scarring predominantly in the inverse areas. The disease is often difficult to treat and patients experience a decreased quality of life (QoL). It is hypothesized that depression is

  3. Body composition and basal metabolic rate in Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Miller, I M; Rytgaard, Helene Charlotte; Mogensen, U B

    2016-01-01

    BACKGROUND: Several studies have suggested an association between Hidradenitis Suppurativa (HS) and obesity. Obesity is often expressed as Body Mass Index (BMI). However, BMI lacks information on body composition. General obesity is a predictor of health status and cardiovascular risk, but body.......70-105.56) (P Hidradenitis Suppurativa is associated with a high fat percentage, high visceral fat, and low muscle percentage adding to the morbidity of HS. The higher predicted estimate of basal metabolic rate (BMR) in HS patients may reflect...

  4. Psychophysical Aspects of Hidradenitis Suppurativa

    National Research Council Canada - National Science Library

    Matusiak, Lukasz; Bieniek, Andrzej; Szepietowski, Jacek C

    2010-01-01

    Hidradenitis suppurativa is a recurrent, debilitating suppurative skin disease. The symptoms are much more than just physical, but studies of its impact on patients' psychological state and related quality of life are very limited...

  5. Genetics Home Reference: hidradenitis suppurativa

    Science.gov (United States)

    ... JN. γ-Secretase mutations in hidradenitis suppurativa: new insights into disease pathogenesis. J Invest Dermatol. 2013 Mar; ... healthcare professional . About Genetics Home Reference Site Map Customer Support Selection Criteria for Links USA.gov Copyright ...

  6. Hidradenitis Suppurativa and Wound Management.

    Science.gov (United States)

    Dini, Valentina; Oranges, Teresa; Rotella, Luca; Romanelli, Marco

    2015-09-01

    Hidradenitis suppurativa (HS) is a chronic, burdensome, debilitating disease of the hair follicle. It presents with recurrent painful inflamed and noninflamed lesions usually in specific body areas such as axillary, inguinal, perineal, and genital areas. It is associated with a large range of other diseases and conditions, such as obesity, arthropathy, inflammatory bowel diseases, and sqaumous cell carcinoma. Medical therapy may be systemic or topical, mainly based on antibiotics, retinoids, hormones and immunosuppressive drugs, including biological therapies. Surgical and laser therapies may be a valid therapeutic approach in order to treat locally recurring lesions. The aim of this article is to review the wound healing options after skin excision and laser treatments, with a focus on lesions left to heal by secondary intention, analyzing the efficacy of moist wound dressings, negative pressure wound therapy, bioactive dressings, such as platelet-rich plasma gel and hylarunoic acid scaffold, or autologous keratinocyte suspension in platelet concentrate and skin-grafting tecniques.

  7. Lasers and Intense Pulsed Light Hidradenitis Suppurativa.

    Science.gov (United States)

    Saunte, Ditte M; Lapins, Jan

    2016-01-01

    Lasers and intense pulsed light (IPL) treatment are useful for the treatment of hidradenitis suppurativa (HS). Carbon dioxide lasers are used for cutting or vaporization of the affected area. It is a effective therapy for the management of severe and recalcitrant HS with persistent sinus tract and scarring, and can be performed under local anesthesia. HS has a follicular pathogenesis. Lasers and IPL targeting the hair have been found useful in treating HS by reducing the numbers of hairs in areas with HS. The methods have few side effects, but the studies are preliminary and need to be repeated.

  8. Surgical Procedures in Hidradenitis Suppurativa

    NARCIS (Netherlands)

    Janse, Ineke; Bieniek, Andrzej; Horvath, Barbara; Matusiak, Lukasz

    2016-01-01

    Hidradenitis suppurativa is difficult to treat owing to its complex pathomechanism; besides the extensive inflammation with abscesses and inflammatory nodules, there is also an architectural loss with sinus tract formation and in severe cases with extensive scarring. Therefore, surgery is mandatory

  9. Surgical Procedures in Hidradenitis Suppurativa

    NARCIS (Netherlands)

    Janse, Ineke; Bieniek, Andrzej; Horvath, Barbara; Matusiak, Lukasz

    Hidradenitis suppurativa is difficult to treat owing to its complex pathomechanism; besides the extensive inflammation with abscesses and inflammatory nodules, there is also an architectural loss with sinus tract formation and in severe cases with extensive scarring. Therefore, surgery is mandatory

  10. Hidradenitis suppurativa : From pathogenesis to emerging treatment options

    NARCIS (Netherlands)

    Dickinson-Blok, Janine Louise

    2015-01-01

    Hidradenitis suppurativa (HS) is a chronic skin disease that is characterized by inflammation of the hair follicles. The cause of HS is largely unknown and the disease remains difficult to treat. Mrs. Janine Dickinson-Blok studied the pathogenesis of HS and the efficacy of existing and emerging ther

  11. Biological and surgical treatment of severe hidradenitis suppurativa

    NARCIS (Netherlands)

    van Rappard, D.C.

    2015-01-01

    Hidradenitis suppurativa (HS) is a chronic, purulent, recurrent, devastating skin disease that affects approximately 1% of the general population. HS typically affects the flexural areas of the body such as the axilla, groin, and perianal area. HS is generally perceived as a difficult skin disease

  12. Leukocyte Profile in Peripheral Blood and Neutrophil-Lymphocyte Ratio in Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Miller, Iben Marie; Ring, Hans Christian; Prens, Errol P.

    2016-01-01

    Background: Hidradenitis suppurativa (HS) is a chronic, inflammatory skin disease. Increasing evidence suggests that HS involves dysfunctional immune responses in both the adaptive and the innate immune system. The recently proposed association of HS with metabolic syndrome may further add...

  13. Gallstones are associated with hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Miller, Iben M; Vindsand Naver, Astrid; Mogensen, Ulla B

    2015-01-01

    BACKGROUND: Hidradenitis suppurativa (HS) is a chronic inflammatory dermatological disease that was recently linked to the metabolic syndrome (MetS). MetS has been associated with gallstones, and nonalcoholic fatty liver has been suggested to be the hepatic expression of MetS. OBJECTIVE: The obje......BACKGROUND: Hidradenitis suppurativa (HS) is a chronic inflammatory dermatological disease that was recently linked to the metabolic syndrome (MetS). MetS has been associated with gallstones, and nonalcoholic fatty liver has been suggested to be the hepatic expression of MetS. OBJECTIVE......: The objective of the study was to investigate whether there was an association of HS with gallstones as well as with hepatic dysfunction. PATIENTS AND METHODS: This was a cross-sectional study comparing a hospital-based HS group, a population-based HS group, and controls for self-reported gallstone and blood...... identified. The age-sex-smoking-adjusted analysis of gallstones revealed a significant OR of 1.72 (95% CI 1.23-2.42, P=0.0191) and a borderline significant OR of 3.28 (95% CI 1.24-8.74, P=0.0516) for the population HS group and hospital HS group versus controls, respectively. Furthermore, no clinically...

  14. Renal amyloid A amyloidosis as a complication of hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Schandorff, Kristine D; Miller, Iben M; Krustrup, Dorrit;

    2016-01-01

    Rheumatic disease is the dominant cause of amyloid A (AA) amyloidosis, but other chronic inflammatory diseases may have similar consequences. Hidradenitis suppurativa (HS) is a relatively common, but little known skin disease characterized by chronic inflammation. Here we present a case of chroni...... HS leading to biopsy-verified severe renal AA amyloidosis and dialysis dependency....

  15. Hidradenitis suppurativa : Pathogenesis, burden of disease and surgical strategies

    NARCIS (Netherlands)

    Janse, Ineke Christina

    2016-01-01

    The general aim of this thesis was to gain more knowledge about the cause, burden and surgical treatment of hidradenitis suppurativa (HS). HS is a chronic, debilitating inflammatory skin disease affecting the inguinal, axillary and gluteal regions. The disease usually develops after puberty, and the

  16. What happens after a single surgical intervention for hidradenitis suppurativa?

    DEFF Research Database (Denmark)

    Jemec, Gregor B E; Guérin, Annie; Kaminsky, Michael

    2016-01-01

    Objective Hidradenitis suppurativa (HS) is often treated by surgery. The risk of recurrence after surgery is common and the consequences are substantial, but neither has been quantified using a claims database. This study aimed to estimate the burden associated with non-curative surgery in HS...

  17. New insights into the diagnosis of hidradenitis suppurativa

    DEFF Research Database (Denmark)

    van der Zee, Hessel H; Jemec, Gregor B E

    2015-01-01

    Current classifications for hidradenitis suppurativa (HS), such as the Hurley staging system, are not very specific. The absence of rigorous classifications is incongruous with the clinical variability of the condition. There is no pathognomonic sign for HS that leads to a diagnosis...

  18. Hidradenitis suppurativa : Pathogenesis, burden of disease and surgical strategies

    NARCIS (Netherlands)

    Janse, Ineke Christina

    2016-01-01

    The general aim of this thesis was to gain more knowledge about the cause, burden and surgical treatment of hidradenitis suppurativa (HS). HS is a chronic, debilitating inflammatory skin disease affecting the inguinal, axillary and gluteal regions. The disease usually develops after puberty, and the

  19. Depression in patients with hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Onderdijk, A J; van der Zee, H H; Esmann, S

    2013-01-01

    BACKGROUND: Hidradenitis suppurativa (HS) is a chronic recurrent inflammatory skin disease with abscess formation and scarring predominantly in the inverse areas. The disease is often difficult to treat and patients experience a decreased quality of life (QoL). It is hypothesized that depression...... is more common in HS patients than among other dermatological patients. OBJECTIVES: To evaluate the prevalence of depression in patients with HS. METHODS: In total 211 HS patients were included in the study and 233 were dermatological control patients. Their QoL and depression scores were assessed using...... the Dermatology Life Quality Index (DLQI) and the Major Depression Inventory (MDI) questionnaires. HS severity was recorded with a questionnaire and Hurley stages were extracted from the case records. RESULTS: The DLQI was significantly higher for HS patients than for the control patients, 8.4 ± 7.5 vs. 4.3 ± 5...

  20. [Ultrasound examination of hidradenitis suppurativa].

    Science.gov (United States)

    Martorell, A; Segura Palacios, J M

    2015-11-01

    Hidradenitis suppurativa is a debilitating chronic, recurrent, inflammatory cutaneous disease of the hair follicle that usually presents with painful, deep and inflamed lesions in the areas of the body with apocrine glands, most frequently the axillary, groin and anogenital regions. This entity is difficult to manage since it can be difficult to determine the true nature and extension of the lesions. Cutaneous ultrasound allows real-time visualization of the cutaneous structures under examination, defining the type of lesion, its anatomical extension, and the degree of inflammatory activity, which affects adequate patient management. The present review analyses the importance of ultrasound in the assessment of patients with hidradenitis suppurativa. Copyright © 2015 Academia Española de Dermatología y Venereología. Published by Elsevier España, S.L.U. All rights reserved.

  1. Association of Metabolic Syndrome and Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Miller, Iben Marie; Ellervik, Christina; Vinding, Gabrielle Randskov

    2014-01-01

    ,predominantly female, and more often smokers compared with the non-HS group.EXPOSURE Hidradenitis suppurativa.MAIN OUTCOMES AND MEASURES Metabolic syndrome and its components of diabetes mellitus, hypertension, dyslipidemia, and obesity.RESULTS When compared with the non-HS group, the odds ratios (ORs......IMPORTANCE An association between the metabolic syndrome (MetS) and chronic inflammatory diseases, such as psoriasis or rheumatoid arthritis, has been suggested.Hidradenitis suppurativa (HS), a more localized chronic inflammation of the skin, has been speculated to have a similar association......) for the hospital HS and population HS groups were 3.89 (95%CI, 1.90-7.98) and 2.08 (95%CI, 1.61-2.69),respectively, for MetS; 5.74 (95%CI, 1.91-17.24) and 2.44 (95%CI, 1.55-3.83), respectively, for diabetes mellitus; 6.38 (95%CI, 2.99-13.62) and 2.56 (95%CI, 2.00-3.28), respectively, for general obesity; and 3...

  2. Psychosocial impact of hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Esmann, S.; Jemec, G.B.E.

    2011-01-01

    , especially in relation to smell and appearance, various emotional reactions, and feelings of lack of control. It was found that hidra-denitis suppurativa has a great emotional impact on patients and promotes isolation due to fear of stigmati-zation. Shame and irritation are frequent and relate to smell......, scars, itching and pain. Quality of life is adversely affected and professional support is needed.© 2011 The Authors....

  3. Two Cases of Paradoxical Hidradenitis Suppurativa while on Adalimumab

    Directory of Open Access Journals (Sweden)

    Glenn Harvin

    2016-05-01

    Full Text Available Hidradenitis suppurativa (HS is a chronic, inflammatory skin disease characterized by recurring abscesses, nodules, and fistulas predominantly in the groin and axillae. The association between HS and Crohn’s disease (CD has been well documented. Tumor necrosis factor (TNF inhibitors have shown to be effective in treating both HS and CD. We report 2 patients who developed HS while on TNF inhibitor treatment for CD.

  4. Is mechanical stress an important pathogenic factor in hidradenitis suppurativa?

    NARCIS (Netherlands)

    K. de Winter (K.); H.H. van der Zee (Hessel); E.P. Prens (Errol)

    2012-01-01

    textabstractHidradenitis suppurativa (HS) is a chronic of recurrent, inflammatory, follicular disease that usually presents after puberty with painful deep-seated, inflamed lesions in the inverse skin areas of the body. It has been hypothesized that mechanical pressure or friction is a risk factor f

  5. Is hidradenitis suppurativa associated with anaemia?

    DEFF Research Database (Denmark)

    Miller, I. M.; Johansen, M E; Mogensen, U. B.

    2016-01-01

    BACKGROUND: Chronic inflammatory diseases may be associated with anaemia of inflammation. Hidradenitis suppurativa is a chronic inflammatory dermatological disease associated with metabolic comorbidities, low quality of life and fatigue. Anaemia may cause fatigue, and it has been hypothesized...

  6. Psychosocial impact of hidradenitis suppurativa: a qualitative study

    National Research Council Canada - National Science Library

    Esmann, Solveig; Jemec, Gregor B E

    2011-01-01

    Hidradenitis suppurativa influences patients' lives in many ways. It is therefore necessary to focus on the effects of the disease on daily life in order better to define patient-related outcomes in hidradenitis suppurativa studies...

  7. Preliminary findings suggest hidradenitis suppurativa may be due to defective follicular support

    DEFF Research Database (Denmark)

    Danby, F W; Jemec, G B E; Marsch, W Ch

    2013-01-01

    BACKGROUND: The initial pathology in hidradenitis suppurativa (HS)/acne inversa takes place in the folliculopilosebaceous unit (FPSU) and its surrounding tissue. The process involves follicular hyperkeratosis, inflammation and perifolliculitis. Identification of the exact origin of inflammation may...

  8. Imaging of Hidradenitis Suppurativa and Its Complications

    Directory of Open Access Journals (Sweden)

    Feng Poh

    2014-01-01

    Full Text Available We present a 56-year-old man with known diabetes mellitus and a 10-year history of hidradenitis suppurativa (HS and highlight, through MR imaging findings, the relentless natural progression of the disease, characterized by recurrent exacerbations of abscesses and fistulae and complications of perianal fistulae and sacral osteomyelitis. We also demonstrate the appearance of this condition on PET-CT with F-18 FDG, which was performed for staging after postexcision tissue specimen revealed well-differentiated squamous cell carcinoma. The association of arthritis and possible dactylitis was also manifested in our patient. Discussion of HS in the radiology literature is limited and, to our knowledge, there has been no case report describing these imaging findings in the same patient.

  9. Disutility in Patients with Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Riis, Peter Theut; Vinding, Gabrielle Randskov; Ring, Hans Christian

    2016-01-01

    values for the general population in Denmark. A significantly decreased utility in patients with hidradenitis suppurativa was found for all age group levels, except for 65–74-year-olds. The total index score in the cohort was 0.705 (population mean 0.887) and the VAS was 62.25 (population mean 82.......6). Multivariate analysis found significant associations between loss of utility and pain, malodour and pruritus (p hidradenitis suppurativa had a significantly decreased EQ-5D compared with the background population. Malodour and pruritus were found to be associated with low index values......Disutility reflects the disability caused by a disease. The EuroQoL-5D (EQ-5D) questionnaire is a measure of health-related overall utility. The questionnaire has only been applied previously to a small number of patients with hidradenitis. In this study a survey of 421 patients with hidradenitis...

  10. Lithium induced hidradenitis suppurativa and acne conglobata

    Directory of Open Access Journals (Sweden)

    Aithal Vijay

    2004-09-01

    Full Text Available Lithium is known to cause a variety of dermatological problems, common ones being acneiform eruptions, folliculitis and psoriasis including its pustular form. Hidradenitis suppurativa and acne conglobata are lesser known side effects, with only three reports so far. We report a patient, who had bipolar affective disorder, was on lithium for a long duration and developed hidradenitis suppurativa and acne conglobata during therapy, which subsequently decreased once lithium was stopped. We describe this case for its rarity and analyze its pathogenesis.

  11. Novel Technique for Management of Axillary Hidradenitis Suppurativa Using Setons

    Directory of Open Access Journals (Sweden)

    Sepehr Seyed Lajevardi

    2015-01-01

    Full Text Available Management of hidradenitis suppurativa (HS of the axilla which is nonresponding to conservative management presents a significant therapeutic challenge. Most surgical treatment options are associated with significant morbidities and prolonged hospital stay. We present a technique of management of HS using setons which is simple and allows the ongoing treatment to be done on an outpatient basis. Given the fact that HS is a chronic relapsing condition each recurrence may again be managed using this technique. This will allow the patients to manage their recurrences with minimal impact on their activities of daily living.

  12. Hidradenitis suppurativa and acne conglobata associated with spondyloarthropathy.

    Science.gov (United States)

    Leybishkis, B; Fasseas, P; Ryan, K F; Roy, R

    2001-03-01

    Hidradenitis suppurativa and acne conglobata are well-described chronic dermatologic diseases. Although the exact incidence of these disorders is unknown, both are relatively uncommon conditions. The incidence of spondyloarthropathy is less than 1% in the general population. Therefore, a triad of hidradenitis suppurativa, acne conglobata and spondyloarthropathy is a rare syndrome described only in a few case reports in the literature. We report a case of hidradenitis suppurativa and acne conglobata associated with spondyloarthropathy.

  13. Risk of Major Adverse Cardiovascular Events and All-Cause Mortality in Patients With Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Egeberg, Alexander; Gislason, Gunnar H; Hansen, Peter R

    2016-01-01

    IMPORTANCE: Hidradenitis suppurativa (HS) is a common inflammatory skin disease. The disease has been associated with cardiovascular (CV) risk factors, but the risk of CV disease in patients with HS is unknown. OBJECTIVE: To investigate CV risk in patients with HS. DESIGN, SETTING, AND PARTICIPAN...

  14. Identification of Clinical and Genetic Parameters Associated with Hidradenitis Suppurativa in Inflammatory Bowel Disease

    NARCIS (Netherlands)

    Janse, Ineke C; Koldijk, Marjolein J; Spekhorst, Lieke M; Vila, Arnau Vich; Weersma, Rinse K; Dijkstra, Gerard; Horváth, Barbara

    2016-01-01

    BACKGROUND: Hidradenitis suppurativa (HS) has recently been associated with inflammatory bowel disease (IBD). The objective of this study is to investigate the prevalence of HS in IBD and to identify clinical and genetic parameters associated with HS in IBD. METHODS: A questionnaire, validated for H

  15. Prevalence and risk of inflammatory bowel disease in patients with hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Egeberg, Alexander; Jemec, Gregor B E; Kimball, Alexa B

    2017-01-01

    Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease. In small studies, inflammatory bowel disease (IBD) has been associated with increased prevalence of HS, but data on the concurrence of IBD in patients with HS is limited. We therefore investigated the prevalence and risk of IBD...

  16. Identification of Clinical and Genetic Parameters Associated with Hidradenitis Suppurativa in Inflammatory Bowel Disease

    NARCIS (Netherlands)

    Janse, Ineke C.; Koldijk, Marjolein J.; Spekhorst, Lieke M.; Vila, Arnau Vich; Weersma, Rinse K.; Dijkstra, Gerard; Horvath, Barbara

    Background: Hidradenitis suppurativa (HS) has recently been associated with inflammatory bowel disease (IBD). The objective of this study is to investigate the prevalence of HS in IBD and to identify clinical and genetic parameters associated with HS in IBD. Methods: A questionnaire, validated for

  17. Compartmentalized Cytokine Responses in Hidradenitis Suppurativa.

    Directory of Open Access Journals (Sweden)

    Theodora Kanni

    Full Text Available Favorable treatment outcomes with TNF blockade led us to explore cytokine responses in hidradenitis suppurativa (HS.Blood monocytes of 120 patients and 24 healthy volunteers were subtyped by flow cytometry. Isolated blood mononuclear cells (PBMCs were stimulated for cytokine production; this was repeated in 13 severe patients during treatment with etanercept. Cytokines in pus were measured.CD14brightCD16dim inflammatory monocytes and patrolling monocytes were increased in Hurley III patients. Cytokine production by stimulated PBMCs was low compared to controls but the cytokine gene copies did not differ, indicating post-translational inhibition. The low production of IL-17 was restored, when cells were incubated with adalimumab. In pus, high concentrations of pro-inflammatory cytokines were detected. Based on the patterns, six different cytokine profiles were discerned, which are potentially relevant for the choice of treatment. Clinical improvement with etanercept was predicted by increased production of IL-1β and IL-17 by PBMCs at week 8.Findings indicate compartmentalized cytokine expression in HS; high in pus but suppressed in PBMCs. This is modulated through blockade of TNF.

  18. Hidradenitis suppurativa: from pathogenesis to diagnosis and treatment

    Directory of Open Access Journals (Sweden)

    Napolitano M

    2017-04-01

    Full Text Available Maddalena Napolitano,1 Matteo Megna,2 Elena A Timoshchuk,3 Cataldo Patruno,2 Nicola Balato,2 Gabriella Fabbrocini,2 Giuseppe Monfrecola2 1Dipartimento di Medicina e Scienze della Salute “Vincenzo Tiberio”, Università degli Studi del Molise, Campobasso, 2Section of Dermatology, Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy; 3Department of Dermatovenereology, Saint Petersburg State Pediatric Medical University, Saint Petersburg, Russia Abstract: Hidradenitis suppurativa (HS is a chronic inflammatory skin disease primarily affecting apocrine gland-rich areas of the body and presenting with painful nodules, abscesses, sinus tracts, and scarring. HS is a multifactorial disease in which genetic and environmental factors play a key role. The primary defect in HS pathophysiology involves follicular occlusion of the folliculopilosebaceous unit, followed by follicular rupture, and immune responses (perifollicular lympho-histiocytic inflammation, finally leading to the development of clinical HS lesions. HS has a destructive impact on the patient’s quality of life, being a very challenging disease. Available treatments are limited, mostly off-label and with high variability in the reported efficacy. Fortunately, a monoclonal antibody against tumor necrosis factor alpha has been recently approved for treatment of moderate to severe HS, offering patients a promising new option. This review focuses on the main features of HS, including epidemiology, clinical aspects, pathogenesis, severity classifications, comorbidities, and currently available treatments. Keywords: hidradenitis suppurativa, pathogenesis, diagnosis, treatment

  19. Adalimumab for the treatment of moderate to severe hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Kimball, A.B.; Kerdel, F.; Adams, D.

    2012-01-01

    Background: Hidradenitis suppurativa (HS) is a chronic, painful skin disease characterized by abscesses, nodules, and draining fistulas in the axilla and groin of young adults. Objective: To evaluate the efficacy and safety of adalimumab, an anti-tumor necrosis factor-αantibody, in patients......Trials.gov: NCT00918255) Setting: 26 academic and private practice medical centers in the United States and Europe. Patients: 154 adult patients with moderate to severe HS who were unresponsive or intolerant to oral antibiotics. Intervention: Patients were assigned in a 1:1:1 ratio to adalimumab, 40 mg...

  20. A review of wide surgical excision of hidradenitis suppurativa

    Directory of Open Access Journals (Sweden)

    Alharbi Ziyad

    2012-06-01

    Full Text Available Abstract Background Hidradenitis suppurativa (HS is a chronic inflammatory cutaneous disorder that involves the infundibular terminal follicles in areas rich of apocrine glands. It can be associated with fistulating sinus, scarring and abscesses formation. Hidradenitis suppurativa is a challenging aspect and requires a proper treatment plan which may involve different specialties. We present herein the option of surgical treatment involving wide surgical excision and methods of reconstruction as well as the rate of recurrence. Furthermore, review of the literature regarding surgical treatment of hidradenitis suppurativa is provided. Methods A retrospective analysis reviewed 50 operative procedures for 32 patients in 5 anatomical sites. These anatomical sites have been divided to 23 sites involving the axilla, 17 sites involving the inguinal region and 8 sites involving the perianal/perineal area, 1 site involving the gluteal region and 1 site involving the trunk region. Results Twenty six patients (81, 25 % showed no recurrence after surgery and the average time of hospital stay period was 5 days. Recurrence was observed only in 6 patients (18, 75 %. Conclusion Elimination of the acute inflammatory process should occur in advance, including the use of antibiotics and minor surgeries such as abscess drainage with proper irrigations. After stabilizing the acute phase, wide surgical excision is recommended. Herein, planning of surgical reconstruction should be initiated to achieve the best outcome and consequently decreasing the risk of recurrence and complications after surgery.

  1. Hidradenitis suppurativa: literature review and case report

    Directory of Open Access Journals (Sweden)

    Mônica Mourthé de Alvim Andrade

    2012-06-01

    Full Text Available Hidradenitis suppurativa (HS is a chronic, recurrent and debilitating disease, affecting mainly women, especially in their second and third decades of life. Its most common incidence is in the axillary, inguinal, perianal and inframammary regions. Its complications include chronic fistulizing processes, with involvement of important adjacent structures, such as the sacrum and coccyx, the anal sphincter, urethra and great-caliber vessels, such as the groin vessels. The proportions of some cases of HS requiring extensive surgical procedures at several moments and the application of flaps and grafts, justify unusual cases reports, like this one. The authors present a case of extensive involvement of the perianal and gluteal regions, which required extended resection with flap in the first approach and fistulectomy in a second surgical moment, with good result for the patient.A hidradenite supurativa (HS é uma doença crônica, recorrente e debilitante, que afeta principalmente mulheres, sobretudo na segunda e terceira décadas de vida. Incide mais nas regiões axilar, inguinal, perianal e inframamária. Dentre suas complicações crônicas, são descritos processos fistulizantes com comprometimento de estruturas importantes, como o sacro e o cóccix, aparelho esfincteriano, uretra e vasos calibrosos, como os inguinais. As proporções de alguns casos de HS, exigindo intervenções alargadas, em vários tempos e com aplicação de retalhos e enxertos justificam relatos de casos que fogem da rotina, como o atual. Os autores apresentam um caso de comprometimento extenso da região glútea e perianal, que exigiu ressecção alargada com retalho e fistulectomia em um segundo tempo cirúrgico, com bom resultado para a paciente.

  2. A Population- and Hospital-based Cross-sectional Study of Renal Function in Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Miller, Iben M; Carlson, Nicholas; Mogensen, Ulla Brasch

    2016-01-01

    The chronic inflammatory skin diseases hidradenitis suppurativa (HS) and psoriasis have been linked to cardiovascular risk factors and the latter has also been linked to possible renal dysfunction. Since basement membrane thinning in the skin of HS patients has been described, we speculated whether...

  3. Ustekinumab in hidradenitis suppurativa : clinical results and a search for potential biomarkers in serum

    NARCIS (Netherlands)

    Blok, J. L.; Li, K.; Brodmerkel, C.; Horvatovich, P.; Jonkman, M. F.; Horvath, B.

    Background Treatment of hidradenitis suppurativa (HS) is difficult and the search for effective therapies continues. Objectives To evaluate the efficacy of ustekinumab and to discover a potential biomarker for HS. Methods Seventeen patients were included in this open-label study and treated with 45

  4. Hidradenitis Suppurativa and Crohn’s Disease: A Case Series

    LENUS (Irish Health Repository)

    Kirthi, S

    2017-09-01

    Hidradenitis Suppurativa (HS) is characterized by chronic recurrent abscesses, nodules and draining sinus tracts with scar formation. Cutaneous Crohn’s Disease (CD) may also present similarly. We wished to identify and describe an Irish cohort with combined HS and CD, with a view to a better recognition of clinical manifestations and understanding of the pathophysiology underlying these two overlapping conditions. Cases were identified using the HIPE Code at Tallaght Hospital from 1990-2014 and retrospective review was performed. Seven patients with both HS and CD were identified, 5(71%) female. The median age of diagnosis with both conditions was 37 years. In all cases, CD had preceded the diagnosis of HS. All patients smoked. Six had an increased BMI and 43% had additional autoimmune conditions. All patients required treatment with a TNF-alpha inhibitor for HS with 5 of 6 subjects having reduced frequency of flare ups and clinically less active HS on follow up

  5. Pathogenesis: common pathways between hidradenitis suppurativa and Crohn disease.

    Science.gov (United States)

    García Martínez, F J; Menchén, L

    2016-09-01

    Both hidradenitis suppurativa and Crohn disease are considered chronic inflammatory diseases due to immune dysregulation. The high prevalence of Crohn disease patients diagnosed with hidradenitis suppurativa suggests the existence of common pathogenic links. The present literature review analyses the similarities and differences in the pathogenesis of the two diseases, in the search for new research and knowledge targets.

  6. Two phase 3 trials of adalimumab for hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Kimball, Alexa Boer; Okun, Martin; Williams, David A

    2016-01-01

    BACKGROUND Hidradenitis suppurativa is a painful, chronic inflammatory skin disease with few options for effective treatment. In a phase 2 trial, adalimumab, an antibody against tumor necrosis factor á, showed efficacy against hidradenitis suppurativa. METHODS PIONEER I and II were similarly desi...

  7. The Association between Hidradenitis Suppurativa and Crohn's Disease

    DEFF Research Database (Denmark)

    van der Zee, Hessel H; Horvath, Barbara; Jemec, Gregor B E

    2016-01-01

    Hidradenitis suppurativa is a chronic, autoinflammatory skin disease. Shalom et al. demonstrate in a large cross-sectional study an association between Crohn's disease and hidradenitis suppurativa, but not with ulcerative colitis. This association supports the hypothesis that a similar pathogenic...

  8. Evidence-based approach to the treatment of hidradenitis suppurativa/acne inversa, based on the European guidelines for hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Gulliver, Wayne; Zouboulis, Christos C.; Prens, Errol

    2016-01-01

    Hidradenitis suppurativa/acne inversa (HS) is a chronic inflammatory skin disease characterized by painful, recurrent nodules and abscesses that rupture and lead to sinus tracts and scarring. To date, an evidence-based therapeutic approach has not been the standard of care and this is likely due ...

  9. Evidence-based approach to the treatment of hidradenitis suppurativa/acne inversa, based on the European guidelines for hidradenitis suppurativa

    NARCIS (Netherlands)

    W.P. Gulliver (Wayne P.); C.C. Zouboulis (Christos C.); E.P. Prens (Errol); G.B.E. Jemec (Gregor); T. Tzellos (Thrasivoulos)

    2016-01-01

    textabstractHidradenitis suppurativa/acne inversa (HS) is a chronic inflammatory skin disease characterized by painful, recurrent nodules and abscesses that rupture and lead to sinus tracts and scarring. To date, an evidence-based therapeutic approach has not been the standard of care and this is li

  10. A Case of Relapsing Polyarthritis Associated With Hidradenitis Suppurativa

    Directory of Open Access Journals (Sweden)

    Avinash Adiga MD

    2016-11-01

    Full Text Available Hidradenitis suppurativa (HS is a chronic, inflammatory follicular skin disease with recurrent skin nodules, sinus tracts, and scarring. We observed a case of HS associated with relapsing polyarthritis. On presentation the patient had a flare of polyarthritis with an increase in the number and size of pustular nodules. He has had similar episodes 1 to 2 times yearly subsiding with antibiotic treatment. Radiographs revealed erosions and demineralization. Symptoms improved following institution of anti-inflammatory and antibiotic therapy. HS is associated with several inflammatory conditions, and dysregulation in innate immunity may play an important role in etiopathogenesis. Spondyloarthritis/sacroiliitis is the most common joint manifestation in HS and mechanism(s underlying arthropathy is unknown. Treatment of arthritis in HS is anecdotal.

  11. Disseminated Mycobacterium chelonae infection: Complicating a case of hidradenitis suppurativa

    Directory of Open Access Journals (Sweden)

    Satyadarshi Patnaik

    2013-01-01

    Full Text Available Mycobacteium chelonae is a rapidly growing atypical mycobacteria known to be pathogenic in humans. We report a case of Hidradenitis Suppurativa (HS with diabetes complicated by infection of the lesions with Staphylococcus aureus and M. chelonae leading to non-healing and discharging lesions. HS is a rare, insidious and debilitating disease characterized by swollen, painful, inflamed lesions in the axillae, groin, and other parts of the body that contain apocrine glands. Discharge from HS lesions are often found to be sterile, however, polymicrobial bacterial colonization commonly occurs within sinus tracts which can lead to offensive smelling discharge, infection, cellulitis, and superinfection. The incidence of HS is very low and the association with M. chelonae makes it a rare and interesting case.

  12. Scroto-perineal hidradenitis suppurativa complicated by giant scrotal elephantiasis

    Directory of Open Access Journals (Sweden)

    Badr Alharbi

    2015-01-01

    Full Text Available Scrotal elephantiasis has been a recognized complication of inguinal node irradiation, filarial infection, tumor involvement and surgical lymphatic destruction, but has rarely been reported in association with hidradenitis suppurativa (HS. HS, also known as acne inversa, is a chronic and often debilitating disease primarily affecting the axillae, inframammary regions and perineum. The location of the lesions may lead to social embarrassment and failure to seek medical treatment. Scroto-preineal HS complicated by scrotal elephantiasis is a distressing disease. Excisional surgery with reconstruction is the recommended treatment with a high likelihood of good outcome. We present a 38-year-old male patient with long-standing scroto-perineal HS complicated by giant scrotal elephantiasis.

  13. Objective scoring of hidradenitis suppurativa reflecting the role of tobacco smoking and obesity

    DEFF Research Database (Denmark)

    Sartorius, K; Emtestam, L; Jemec, G B E

    2009-01-01

    BACKGROUND: Hidradenitis suppurativa (HS) is a long-standing disease with abscess and often fistula formation, predominantly in the axillae and groins. The disease is difficult to treat and has a severe impact on quality of life. A clinically relevant system for scoring disease severity is lackin...

  14. Hidradenitis suppurativa : A disease of infundibular epidermis rather than pilosebaceous units?

    NARCIS (Netherlands)

    Janse, I. C.; Blok, J. L.; Diercks, G. F. H.; Horvath, B.; Jonkman, M. F.

    although the exact pathogenesis of hidradenitis suppurativa (HS) is unknown, it is established that it is primarily an infundibulitis.(1,2) We consider infundibulum as epidermis and not as hair follicle according to Ackerman, as the type of epithelial tissue of infundibular epidermis is

  15. Primary cutaneous plasmacytosis: Masquerading as hidradenitis suppurativa

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    Tarang Goyal

    2016-01-01

    Full Text Available Isolated cutaneous plasmacytosis (CP is a rare entity with few cases reported in world literature. CP masquerading as hidradenitis suppurativa like presentation is a unique case with some features differentiating it clinically from it which were further confirmed by histopathology and immunostaining. Our case showed hyperplasia of mature plasma cells and polyclonal hypergammaglobulinemia, immunostaining for CD138 positivity and kappa: lambda ratio more than 3:1. Extensive clinical and laboratory investigations failed to reveal any underlying pathology, presence of any underlying disease accompanying the hypergammaglobulinemia and/or plasma cell proliferation.

  16. Hidradenitis suppurativa and perianal Crohn disease: differential diagnosis.

    Science.gov (United States)

    Bassas-Vila, J; González Lama, Y

    2016-09-01

    The first description of perianal fistulas and complications in Crohn disease was made 75 years ago by Penner and Crohn. Published studies have subsequently confirmed that perianal fistulas are the most common manifestations of fistulising Crohn disease. Hidradenitis suppurativa was described in 1854 by a French surgeon, Aristide Verneuil. It is a chronic, inflammatory, recurrent and debilitating disease of the pilosebaceous follicle, that usually manifests after puberty with deep, painful and inflamed lesions in the areas of the body with apocrine glands, usually the axillary, inguinal and anogenital regions. The differential diagnosis between hidradenitis suppurativa and Crohn disease can be challenging, especially when the disease is primarily perianal. When they occur simultaneously, hidradenitis suppurativa and Crohn disease show severe phenotypes and patients can respond to anti-tumour necrosis factor therapy, although adalimumab is currently the only treatment with demonstrated efficacy in hidradenitis suppurativa and Crohn disease. In addition, there is sometimes a need for different complementary surgical procedures.

  17. Hidradenitis suppurativa in Down’s syndrome: A case report

    Directory of Open Access Journals (Sweden)

    Nadeem Toodayan

    2015-07-01

    Full Text Available Of the many dermatological conditions thought to be linked with Down’s syndrome, hidradenitis suppurativa presents a peculiar manifestation. This brief case report summarises the clinical features and possible genetic basis for this fascinating association.

  18. A 3D ultrasound study of sinus tract formation in hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Wortsman, Ximena; Jemec, Gregor

    2013-01-01

    Imaging of hidradenitis suppurativa allows the study of both the lesion morphology and evolution. Hidradenitis lesions of different stages were studied using 3D ultrasound in a cross sectional pilot study. A total of 25 HS patients (18 female/ 7 male, aged 18-46 year-old) and 10 healthy controls (5...... and characterization of the morphological changes in hidradenitis, which include the variable degrees of involvement of the hair follicles and the appearance of fluid collections and sinus tracts. Knowledge of this anatomical information may allow the identification of lesions that can benefit from medical or surgical...

  19. Hidradenitis suppurativa in a HIV-infected child

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    G Prabhu

    2012-01-01

    Full Text Available Hidradenitis suppurativa (HS, a painful and chronic condition, commonly occurs in women and coincides with post-pubertal increase in sex hormones. A 13-year-old pre-pubertal HIV-infected male child presented to our clinic with a discharging right axillary lymph node swelling. The biopsy of the lesion showed features of HS. The patient was treated with oral antibiotics, oral steroids, and local antibiotic wash. Though the patient responded to this treatment, the clinical response was not adequate and the lesion recurred. Subsequently, the child was started on antiretroviral therapy (zidovudine, lamivudine, and nevirapine. Following these medications, the lesions healed and had not recurred till we last examined the child. Thus, this is a rare presentation of a known condition in an HIV-infected pre-pubertal male child, which did not respond to usual modalities of treatment and had to be treated with antiretroviral therapy.

  20. Recurrence rate and patient satisfaction of CO2 laser evaporation of lesions in patients with hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Mikkelsen, Peter Riis; Dufour, Deirde Nathalie; Zarchi, Kian

    2015-01-01

    BACKGROUND: Hidradenitis suppurativa (HS) is a debilitating disease and is difficult to treat. Validation of surgical techniques is therefore of great importance in the management of HS. Carbon dioxide (CO2) laser evaporation has been shown effective, but larger-scale studies are scarce. OBJECTIVE...

  1. Bacterial biofilm in chronic lesions of Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Ring, H C; Bay, L; Nilsson, M

    2017-01-01

    BACKGROUND: Chronic non-healing or recurrent inflammatory lesions, reminiscent of infection but recalcitrant to antibiotic therapy generally characterize biofilm driven-diseases. Chronic lesions of Hidradenitis Suppurativa (HS) exhibit several aspects, which are compatible with well-known biofilm...... Acid (PNA) - Fluorescence in situ Hybridization (FISH) in combination with Confocal Laser Scanning Microscopy (CLSM). In addition, corresponding histopathological analysis in hematoxylin and eosin slides were performed. RESULTS: Biofilms were seen in 67% of the samples of chronic lesions and in 75......% of the perilesional samples. The mean diameter of aggregates in lesional skin was significantly greater than in perilesional skin (p=0.01). Biofilms exceeding 50 μm in diameter were found in 42% of lesional samples and only in only 5% of the perilesional samples (p=0.009). The majority of the large biofilms...

  2. Hidradenitis suppurativa: an update on connecting the tracts [version 1; referees: 3 approved

    Directory of Open Access Journals (Sweden)

    Mallory K Smith

    2017-07-01

    Full Text Available Hidradenitis suppurativa (HS is a devastating disease involving abscesses, sinus tracts, and inflammation classically affecting the axilla, groin, and/or anogenital region. Although the disease pathogenesis is not fully understood, recent advances suggest that HS pathology runs much deeper than the cutaneous manifestations. It is now believed that HS is a systemic inflammatory disease that gives rise to the characteristic cutaneous manifestations. This disease is problematic for both patients and physicians to manage because of a variety of diagnostic and management difficulties. This article seeks to provide updates on the current understanding of HS to increase awareness and improve management.

  3. Review of Current Immunologic Therapies for Hidradenitis Suppurativa

    Directory of Open Access Journals (Sweden)

    Victoria K. Shanmugam

    2017-01-01

    Full Text Available Hidradenitis suppurativa (HS is a chronic, recurrent, inflammatory disease of apocrine gland-bearing skin which affects approximately 1–4% of the population. The disease is more common in women and patients of African American descent and approximately one-third of patients report a family history. Obesity and smoking are known risk factors, but associations with other immune disorders, especially inflammatory bowel disease, are also recognized. The pathogenesis of HS is poorly understood and host innate or adaptive immune response, defective keratinocyte function, and the microbial environment in the hair follicle and apocrine gland have all been postulated to play a role in disease activity. While surgical interventions can be helpful to reduce disease burden, there is a high recurrence rate. Increasingly, data supports targeted immune therapy for HS, and longitudinal studies suggest benefit from these agents, both when used alone and as an adjunct to surgical treatments. The purpose of this review is to outline the current data supporting use of targeted immune therapy in HS management.

  4. Improving quality of life in patients with hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Dufour, D.N.; Esmann, S.; Jemec, G.B.

    2012-01-01

    Hidradenitis suppurativa is a chronic recurrent inflammatory skin disease with abscess formation and scarring predominantly in the inverse areas. The disease is often difficult to treat and patients experience a decreased quality of life (QoL) and related psychosocial morbidity. Current knowledge...

  5. Spotlight on adalimumab in the treatment of active moderate-to-severe hidradenitis suppurativa

    Directory of Open Access Journals (Sweden)

    Fotiadou C

    2016-10-01

    Full Text Available Christina Fotiadou, Efstratios Vakirlis, Dimitrios Ioannides First Department of Dermatology-Venereology, Aristotle University Medical School, Thessaloniki, Greece Abstract: Hidradenitis suppurativa (HS is a chronic, recurrent, inflammatory skin disease that affects the hair follicles of the aprocrine gland-bearing anatomical areas of the body. It is characterized by deep painful nodules and abscesses that rupture and contribute to the formation of sinus tracks and scarring. The management of HS is based on the assessment of disease severity and a combination of medical and surgical treatment according to the European Guidelines. Adalimumab, a recombinant, fully humanized, anti-tumor necrosis factor alpha (anti-TNF-α monoclonal antibody, is the only officially approved treatment for the management of moderate-to-severe HS. Case reports, concerning 42 patients who received adalimumab for severe HS (with the standard dose regimen for psoriasis, reported a cumulative response rate of 58% (≥50% in 23 patients with a relapse rate of 71% (10 out of 14 patients. The most recent and most well-powered phase III, randomized placebo-controlled trials for the evaluation of the efficacy and safety of adalimumab in treatment of moderate-to-severe HS (PIONEER studies I and II showed that the Hidradenitis Suppurativa Clinical Response (HiSCR rate at week 12 was significantly higher for patients randomized to adalimumab compared to placebo. Adverse events were comparable to placebo. In conclusion, adalimumab, to date, holds the most robust data regarding treatment efficacy in HS. Larger, registry-based studies are needed to further establish the efficacy and safety profile of this anti-TNF-α agent in HS. Keywords: hidradenitis suppurativa, adalimumab, treatment

  6. Normal Skin Microbiota is Altered in Pre-clinical Hidradenitis Suppurativa.

    Science.gov (United States)

    Ring, Hans Christian; Bay, Lene; Kallenbach, Klaus; Miller, Iben M; Prens, Errol; Saunte, Ditte M; Bjarnsholt, Thomas; Jemec, Gregor B E

    2017-02-08

    Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease defined by recurrent nodules, tunnels (sinus tracts) and scarring involving the intertriginous regions. The clinical course of HS is compatible with a biofilm-driven disease, and biofilm has been described in lesional HS skin. We therefore hypothesized that clinically unaffected HS skin would also have an increased presence of biofilm compared with that of healthy controls. We conducted a case-control study, investigating the morphology of the axillary skin microbiota. Peptide nucleic acid - fluorescence in situ hybridization probes were used in combination with confocal laser scanning microscopy. Significant differences were found in both distribution and quantity of the cutaneous microbiota in clinically non-affected axillary skin of patients with HS compared with healthy controls. Surprisingly, we detected fewer bacteria and less biofilm in patients with HS. The reduced microbiota in patients with HS may play an important role in the early course of the disease.

  7. Successful Treatment of Recalcitrant Hidradenitis Suppurativa with Adalimumab

    Directory of Open Access Journals (Sweden)

    Ian Gorovoy

    2009-10-01

    Full Text Available Hidradenitis suppurativa is a chronic disease that affects the apocrine gland-bearing regions of the body. The etiology of this disorder is poorly understood, but most likely is a complex process involving follicular apocrine occlusion with subsequent perifolliculitis. Many treatment options have been reported with varying degrees of success, including topical and oral therapy and surgical procedures. Recently, TNF-α antagonists have been reported as effective therapy in a few patients. We report here a patient who initially responded to infliximab but developed an infusion reaction to this medication. After subsequent treatment with adalimumab, the patient’s disease improved dramatically and has been maintained under excellent control for over 15 months. We propose that TNF-α inhibitors, particularly monoclonal antibody based agents, are a viable treatment option in patients with severe, recalcitrant HS and that a patient may be safely and successfully treated with the fully human monoclonal antibody adalimumab in cases in which the chimeric monoclonal antibody infliximab therapy is not tolerated.

  8. The Association between Hidradenitis Suppurativa and Crohn's Disease : in Search of the Missing Pathogenic Link

    NARCIS (Netherlands)

    van der Zee, Hessel H.; Horvath, Barbara; Jemec, Gregor B. E.; Prens, Errol P.

    Hidradenitis suppurativa is a chronic, autoinflammatory skin disease. Shalom et al. demonstrate in a large cross-sectional study an association between Crohn's disease and hidradenitis suppurativa, but not with ulcerative colitis. This association supports the hypothesis that a similar pathogenic

  9. Hidradenite supurativa tratada com infliximabe Hidradenitis suppurativa treated with infliximab

    Directory of Open Access Journals (Sweden)

    Daniel Lago Obadia

    2009-12-01

    Full Text Available A hidradenite supurativa é doença inflamatória de difícil tratamento. Recentemente, a terapia anti-TNF alfa, com anticorpos monoclonais ("terapia biológica", tem sido apontada como uma alternativa. No entanto, ensaios clínicos avaliando a eficácia destas drogas na hidradenite supurativa, ainda não foram publicados. Apresentamos um caso no qual o uso do infliximabe não resultou em melhora clínica expressiva.Hidradenitis suppurativa is an inflammatory disease with difficult treatment. Currently, the anti-TNF alfa therapy, with monoclonal antibodies ("biological therapy", has been shown as a new alternative. However, clinical trials assessing the efficacy of these drugs to treat hidradenitis suppurativa have not yet been published. A case in which the use of infliximab did not result in significant clinical improvement is described.

  10. Radiotherapy of hidradenitis suppurativa - indication today?; Strahlentherapie der Hidradenitis axillaris - heute noch aktuell?

    Energy Technology Data Exchange (ETDEWEB)

    Froehlich, D.; Glatzel, M. [Zentralklinikum, Suhl (Germany). Klinik fuer Strahlentherapie; Baaske, D. [Klinikum Chemnitz (Germany). Klinik fuer Radiologie und Radioonkologie

    2000-06-01

    Purpose: In a retrospective analysis the efficacy of radiotherapy in the treatment of hidradenitis suppurativa was assessed. Patients and Methods: Data from 231 patients undergoing radiotherapy for hidradenitis suppurativa in the last years in 2 hospitals were analyzed. The patients were treated with an orthovoltage therapy unit with 175 kV, 0.5 mm copper-filtering. The single doses reached from 0.5 Gy to 1.5 Gy up to total doses between 3.0 Gy and 8.0 Gy in one series. In chronic recurrent disease 2 or more series with a total dose of more than 10.0 Gy were given. Results: Complete relief of symptoms at the end of radiotherapy was achieved in 89 patients (38%). In 92 patients (40%) there was clear improvement of symptoms. Only 2 patients did not react to radiotherapy. No side effects of radiotherapy occurred. Conclusions: Radiotherapy should be given more consideration as a treatment option for hidradenitis suppurativa. (orig.) [German] Hintergrund: Der Effekt der Strahlenbehandlung der Hidradenitis axillaris wurde am eigenen Patientengut ueberprueft. Patienten und Methode: Es erfolgte eine retrospektive Analyse der Daten von 231 Patienten, die in den letzten Jahren wegen einer Hidradenitis axillaris strahlentherapeutisch in zwei Kliniken behandelt wurden. Die Therapie erfolgte unter Tiefentherapiebedingungen mit Einzeldosen zwischen 0,5 und 1,5 Gy und Gesamtdosen zwischen 3,0 und 8,0 Gy in einer Serie. Nur bei chronisch rezidivierenden Formen wurden Gesamtdosen ueber 10,0 Gy in mehreren Serien eingestrahlt. Ergebnisse: Voellige Beschwerdefreiheit nach Strahlentherapie wurde bei 89 Patienten (38%) erreicht. Weitere 92 Patienten (40%) wiesen eine deutliche Besserung der Symptomatik auf. Eine Abszendierung der entzuendlichen Prozesse trat bei 48 Patienten (21%) ein. Nennenswerte Nebenwirkungen der Behandlung wurden nicht beobachtet. Schlussfolgerung: Die Strahlentherapie sollte auch weiterhin in der Differentialtherapie der Hidradenitis axillaris Beruecksichtigung

  11. Skin-Tissue-sparing Excision with Electrosurgical Peeling (STEEP) : a surgical treatment option for severe hidradenitis suppurativa Hurley stage II/III

    NARCIS (Netherlands)

    Blok, J. L.; Spoo, J. R.; Leeman, F. W. J.; Jonkman, M. F.; Horvath, B.

    2015-01-01

    BackgroundSurgery is the only curative treatment for removal of the persistent sinus tracts in the skin that are characteristic of severe hidradenitis suppurativa (HS). Complete resection of the affected tissue by wide excision is currently regarded as the preferred surgical technique in these cases

  12. The Perils of a Defective Medical Communication: Fatal Neglected Squamous Cell Carcinoma Arising in Perineal Hidradenitis Suppurativa

    Directory of Open Access Journals (Sweden)

    Calogero Pagliarello

    2011-01-01

    Full Text Available Hidradenitis suppurativa (HS is a recurrent suppurative disease characterized by inflammatory nodules, abscesses, fistulas, and scarring. We report a case of squamous cell carcinoma arising in chronic HS. In our case the asymptomatic lesion was neglected by the patient for a long time, allowing the occurrence of advanced disease before treatment was sought, which resulted in a terminal outcome. In the context of HS, a painful lump or ulcer could easily be mistaken for an inflammatory lesion, and therefore a low threshold for biopsy is warranted.

  13. Treatment of hidradenitis suppurativa with intralesional photodynamic therapy with 5-aminolevulinic acid and 630nm laser beam.

    Science.gov (United States)

    Suárez Valladares, María Jesús; Eiris Salvado, Noemi; Rodríguez Prieto, Manuel Angel

    2017-03-01

    Hidradenitis suppurativa (HS) is a chronic relapsing inflammatory skin disease with multiple treatment options that have been used with mixed results. To evaluate the effectiveness, safety and tolerability of intralesional photodynamic therapy (I-PDT) in the management of HS. Also, to assess the effect of this technique on the different areas treated. Case series of 38 HS patients treated with I-PDT between 2011-2015 following a standardized protocol to assess response at the treated areas RESULTS: 29 patients achieved a complete response, while persistence was noted on 8 cases and only 1 suffered a recurrence. Difference between basal (median 28.5) and final (0) Hidradenitis Severity Score showed a significant reduction of 24.5 points (ptreatment option for localized HS lesions, achieving a high rate of remission with an adequate maintenance of response i and few complications. Copyright © 2016 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.

  14. Boils at Frictional Locations in a Patient with Hidradenitis Suppurativa.

    Science.gov (United States)

    Boer, Jurr; Mihajlovic, Dalibor

    2016-12-01

    Dear Editor, Hidradenitis suppurativa (HS) is a chronic, recurrent, inflammatory skin disease. The primary clinical presentation are painful inflamed nodules or boils of inverse areas, i.e. the axillary and anogenito-crural regions, but it can also involve the infra- and inter-mammary regions (1,2). The etiology of HS is not clearly defined. Obesity, smoking, and genetic factors are considered important risk factors. In addition, it has also been suggested that friction may contribute to the development of HS, especially in the obese, but this is based on highly anecdotal reports (3-5). We describe a case with classic HS, obesity, and HS-like lesions at the position of the bra strap, suggesting that mechanical stress was an external pathogenic factor for HS development. A 33-year old woman presented with an 18-year history of chronic, recurrent, inflammatory nodules in the axillae, the groin, the pubic region, and to a lesser extent the abdomen and buttocks. She was obese with as result of 33.2 kg/m-2 of 33.2, had a positive family history of two first grade family members with HS, and was a smoker (19 Pack years). There were no other known comorbidities. The inflamed lesions had been treated with several courses of oral antibiotics (minocycline, erythromycin, and combination therapy of clindamycin and rifampicine) and surgical treatments: lancing, deroofing, and excisions (2,6). On examination, there were nodules, folliculitis, cysts, and depressed scars in the axillae and groins, including the inner thighs (Figure 1). On the chest, corresponding to where the lower edge of the patient's bra was usually located, a superficial nodule and follicular papules were observed, exactly coinciding with the red stripe caused by mechanical stress (friction and pressure) of the bra edge. There was no skin fold present on the location of the HS lesions, and there were no lesions observed in the intermammary region or on the side of the breasts in contact with the skin of the

  15. Association of hidradenitis suppurativa and familial Mediterranean fever: A case series of 6 patients.

    Science.gov (United States)

    Abbara, Salam; Georgin-Lavialle, Sophie; Stankovic Stojanovic, Katia; Bachmeyer, Claude; Senet, Patricia; Buob, David; Audia, Sylvain; Delcey, Véronique; Fellahi, Soraya; Bastard, Jean-Philippe; Awad, Fawaz; Legendre, Marie; Amselem, Serge; Grateau, Gilles

    2017-03-01

    Familial mediterranean fever (FMF) is the most common monogenic autoinflammatory disease. Hidradenitis suppurativa (HS) is an inflammatory cutaneous disease. Those diseases can occur simultaneously among the same individual. Our objective was to describe the features of patients displaying both FMF and HS. We screened the French adult FMF reference center for FMF patients with HS. Six patients out of 151 (4%) with a median age of 36 years old were concerned. Among them, FMF was symptomatic at a median age of 11.5years old and colchicine was introduced at a median age of 20.5years old. HS was diagnosed at a median age of 31.5years old. An elderly patient displayed AA amyloidosis in the outcome of FMF, with a late diagnosis of HS, with response to anakinra. There was no temporal relation between FMF and HS attacks. Some patients had a persistent inflammatory syndrome under treatment. FMF and HS are both inflammatory diseases involving young patients, with HS possibly being an autoinflammatory disease. Although their association seems to be fortuitous, both can induce an important inflammation state that could lead to AA amyloidosis and require a close monitoring of clinical signs and acute-phase reactants. Anakinra was successful in treating the only patient with both HS, FMF and amyloidosis. Copyright © 2016 Société française de rhumatologie. Published by Elsevier SAS. All rights reserved.

  16. An Update on Hidradenitis Suppurativa (Part I): Epidemiology, Clinical Aspects, and Definition of Disease Severity.

    Science.gov (United States)

    Martorell, A; García-Martínez, F J; Jiménez-Gallo, D; Pascual, J C; Pereyra-Rodriguez, J; Salgado, L; Vilarrasa, E

    2015-11-01

    Hidradenitis suppurativa is a chronic inflammatory disorder that has attracted increasing attention in recent years due to underestimations of prevalence and the considerable impact of the condition on interpersonal relationships, physical appearance, self-esteem, and body image. Although hidradenitis suppurative has a significant psychological impact on patients and can even cause physical limitations when thick scarring results in limb mobility limitation, until very recently little evidence was available relating to its epidemiology, etiology, or pathogenesis. In this review, we highlight the latest advances in our understanding of the epidemiological and clinical aspects of hidradenitis suppurativa. We will also look at the different classification systems for hidradenitis suppurativa and discuss the emergence of skin ultrasound as a promising technique for monitoring the course of this chronic inflammatory disease. Copyright © 2015 Elsevier España, S.L.U. and AEDV. All rights reserved.

  17. The Association between Hidradenitis Suppurativa and Crohn's Disease: in Search of the Missing Pathogenic Link.

    Science.gov (United States)

    van der Zee, Hessel H; Horvath, Barbara; Jemec, Gregor B E; Prens, Errol P

    2016-09-01

    Hidradenitis suppurativa is a chronic, autoinflammatory skin disease. Shalom et al. demonstrate in a large cross-sectional study an association between Crohn's disease and hidradenitis suppurativa, but not with ulcerative colitis. This association supports the hypothesis that a similar pathogenic mechanism contributes to both diseases, providing new possibilities for functional studies and therapy development.

  18. Development and initial psychometric evaluation of patient-reported outcome questionnaires to evaluate the symptoms and impact of hidradenitis suppurativa.

    Science.gov (United States)

    Kimball, Alexa B; Sundaram, Murali; Banderas, Benjamin; Foley, Catherine; Shields, Alan L

    2017-07-04

    Two patient-reported outcome (PRO) questionnaires, the Hidradenitis Suppurativa Symptom Assessment (HSSA) and Hidradenitis Suppurativa Impact Assessment (HSIA), were developed to measure signs, symptoms and impacts of HS in treatment efficacy studies. In accordance with FDA guidelines and published best practices, four stages of research were conducted to create the questionnaires: concept elicitation, questionnaire construction, content evaluation and psychometric evaluation. Subjects (N = 20) who participated in the concept elicitation stage reported 15 unique HS-related signs and symptoms and 51 impacts. Following this, eight sign and symptom concepts and 21 impacts were selected for construction of the HSSA and HSIA, respectively. During content evaluation, cognitive debriefing interviews with HS subjects (N = 20) confirmed subjects could read, comprehend and meaningfully respond to both questionnaires. Modifications made after this stage of work resulted in a nine-item HSSA and a 17-item HSIA. The HSSA and HSIA were subsequently entered into a US-based observational study (N = 40), and the scores produced by each were found to be reliable, construct valid, and able to distinguish among clinically distinct groups. The HSSA and HSIA are content-valid, HS-specific, PRO questionnaires with demonstrated ability to generate reliable, valid scores when administered to patients with HS in a research setting.

  19. The Influence of Body Weight on the Prevalence and Severity of Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Kromann, Charles B; Ibler, Kristina S; Kristiansen, Viggo B

    2014-01-01

    The prevalence of hidradenitis suppurativa (HS) has been estimated to be 1% of the population. Obesity is considered a co-morbidity, but the prevalence of HS in obese population is not known. A retrospective questionnaire was distributed to 383 patients over 2 years after bariatric surgery. Data...... on pre- and post-surgery HS symptoms and disease severity were studied. Disease severity was assessed by number of involved sites. General skin problems rated numerically on an anchored 1-10 scale. Valid responses were obtained from 249/383 (65%). A point prevalence of 18.1% (45/249) HS was found....... The number of patients reporting HS symptoms after weight loss decreased by 35% and the mean number of involved sites was reduced from 1.93 to 1.22 following weight loss (p = 0.003). The prevalence of HS appears higher in the obese than in the background population, and a weight loss of more than 15...

  20. Hidradenitis suppurativa and inflammatory bowel disease: are they associated? Results of a pilot study.

    Science.gov (United States)

    van der Zee, H H; van der Woude, C J; Florencia, E F; Prens, E P

    2010-01-01

    Background The co-occurrence of hidradenitis suppurativa (HS) and Crohn disease (CD) published in a few case reports resulted in the wide acceptance of an association between these two diseases. However, the combined prevalence of these diseases is currently unknown; furthermore, it is unknown whether this co-occurrence also applies for ulcerative colitis (UC). Objectives To estimate the prevalence of HS in patients with inflammatory bowel disease (IBD) living in the Southwest of the Netherlands. Methods During an IBD patient information meeting, randomly, 158 patients with IBD were interviewed about recurrent painful boils in the axillae and/or groin and were shown illustrative clinical pictures of the appearance of HS. Results Of the 158 patients interviewed, 102 (65%) had CD and 56 (35%) had UC. Twenty-five people (16%) responded that they had had or still experienced painful boils in the axillae and/or groin, of whom 17 were patients with CD (17%) and eight had UC (14%). Conclusions This pilot study shows for the first time that HS occurs in patients with CD or UC. More prospective studies are warranted to establish the association between HS and IBD and its underlying pathogenesis.

  1. Incidence of hidradenitis suppurativa in the United States: A sex- and age-adjusted population analysis.

    Science.gov (United States)

    Garg, Amit; Lavian, Jonathan; Lin, Gloria; Strunk, Andrew; Alloo, Allireza

    2017-07-01

    The true incidence of hidradenitis suppurativa (HS) is unknown. To determine standardized incidence estimates for HS in the United States. We used a retrospective cohort analysis, including incident HS cases identified using electronic health records data for a demographically heterogeneous population-based sample of >48 million unique patients across all 4 census regions. We calculated standardized 1- and 10-year cumulative incidences for the overall population and for sex-, age-, and race-specific groups. There were 5410 new HS diagnoses over a 1-year period, with an incidence of 11.4 (95% confidence interval [CI], 11.1-11.8) cases per 100,000 population. One-year incidence in women was 16.1 (95% CI, 15.5-16.6) per 100,000, more than twice that of men [6.8 (95% CI, 6.5-7.2) per 100,000; P 2.5 times that of whites [11.7 (95% CI, 11.3-12.2) per 100,000; P over 10 years was 8.6 (95% CI, 8.6-8.7) per 100,000 population. The use of deidentified claims prevented validation for a larger case subset. HS incidence has increased over the past decade and disproportionately involves women, young adults, and African Americans. Copyright © 2017 American Academy of Dermatology, Inc. Published by Elsevier Inc. All rights reserved.

  2. Hedgehog pathway does not play a role in hidradenitis suppurativa pathogenesis

    DEFF Research Database (Denmark)

    Mozeika, E.; Jemec, G.B.E.; Nürnberg, B.M.

    2011-01-01

    Hidradenitis suppurativa is a chronically relapsing skin disorder with onset after puberty and is characterized by inflammatory lesions in hair follicle and apocrine sweat gland-bearing skin that manifests as abscesses with formation of cysts and sinus tracts. Hedgehog family genes are required...... in normal embryonic skin, hair follicle, sebaceous and sweat gland development. Mutations of hedgehog pathway in adult skin have previously been found in basal cell carcinomas and in alopecia as well as in epidermal cysts and in odontogenic keratocysts. Therefore, we suggested that the hedgehog pathway...... might play a role in formation of sinus tracts and cysts as newly formed structures in hidradenitis suppurativa patients. None of the sinus tracts or cysts in 81 hidradenitis suppurativa histological slides from 34 patients showed positive finding for sonic hedgehog mutation. According to our findings...

  3. Diseases associated with hidranitis suppurativa: part 2 of a series on hidradenitis.

    Science.gov (United States)

    Scheinfeld, Noah

    2013-06-15

    Hidradenitis suppurativa (HS), a pathologic follicular disease, impacts patients' lives profoundly and usually occurs in isolation. The diseases with the strongest association are obesity, depression, and pain. HS is associated with many diseases including acne conglobata (AC), dissecting cellulitis, pilonidal cysts, and obesity. Pyoderma fistulans sinifica (fox den disease) appears to be the same entity as Hurley Stage 2 of 3 HS. The rate of acne vulgaris in HS patients mirrors unaffected controls. The most common, albeit still uncommon, association is with seronegative, haplotype unlinked arthritis (most importantly B27), in particular spondolyarthritis. Crohn disease and HS occur together at a rate that varies from 0.6% to 38% in retrospective cases series. Ulcerative colitis occurred with HS in 14% of patients in one series. The next most common association is with pyoderma gangrenosum, but this association is likely under-reported. Synovitis-Acne-Pustulosis Hyperostosis-Osteitis (SAPHO) syndrome, which is rare, has more than 10 reports linking it to HS. Nine case reports have linked Dowling-Degos disease (DDD) to HS and two reports related HS to Fox-Fordyce disease (FF), but because both occur in the axilla this might be a mere coincidence. HS is rarely associated with ophthalmic pathology. Specifically, more than 5 reports link it to Keratitis-Ichthyosis-Deafness syndrome (KID); greater than10 cases link it to interstitial keratitis and 2 cases are linked to Behçet's disease. The presence of proteinuria and acute nephritis link HS to the kidney, especially since and reports have documented resolution of HS after renal transplant. Florid steatocystoma multiplex, Sjogren Syndrome, and HS have been linked and their reports likely underestimate their coincidence because all these entities involve occlusion (albeit by different mechanisms). Three reports link HS and amyloid, but both share some common genetic underpinnings and thus the coincidence of these

  4. Axillary Reconstruction for Hidradenitis Suppurativa with an Inner-Arm Transposition Flap Creating a Brachioplasty Effect

    Directory of Open Access Journals (Sweden)

    Daniel L. Ching

    2017-05-01

    Full Text Available BackgroundHidradenitis suppurativa (HS is a chronic skin condition that can affect any area with apocrine sweat glands and has the potential to involve multiple sites concurrently. Commonly affected sites include the axilla, groin, perineum and perianal areas. In this study we performed a literature review on the surgical methods for HS and describe an innovative technique for reconstructing axilla HS using an inner-arm transposition flap.MethodsWe reviewed all cases (5 cases from 4 patients of transposition flap reconstruction performed by the senior author at a single London tertiary hospital from 2008–2013. Patient related outcome measures were collected using the Derriford appearance scale (DAS 24 and a study specific questionnaire.ResultsAll patients were satisfied with their final result. One out of five cases had a complication but did not result in flap failure. There is no disease recurrence to date. DAS 24 scores collected demonstrated acceptable postoperative distress that did not deviate far from the norm tables while study specific questionnaire reveal desirable outcomes.ConclusionsWe have managed to achieve our aim through the use of the innovative inner-arm transposition flap. Our study hopes to provide an additional technique for axillary reconstruction. This technique offers the effective concealment of scars with the benefit of tightening of the arm tissue producing ‘brachioplasty like’ effects. All things considered it would be reasonable to conclude the innovative flap technique is a reliable, effective, and simple method that results in multiple benefits.

  5. A systematic review of the use of lasers for the treatment of hidradenitis suppurativa.

    Science.gov (United States)

    John, Hannah; Manoloudakis, Nikolaos; Stephen Sinclair, J

    2016-10-01

    To conduct a systematic review of the effectiveness of various types of lasers (and light based therapies) for the treatment of hidradenitis suppurativa (HS) and to establish recommendations based on our findings. MEDLINE, Cochrane and PubMed databases. English language studies describing the use of laser for the treatment of HS. Multiple reviewers performed independent extraction and identified 22 studies that met the inclusion and exclusion criteria. Studies were categorised according to grading recommendations based on evidence quality guidelines for systematic reviews. Only 2 studies met criteria to be assigned the highest grade. Nd:YAG laser has been shown to be effective for the treatment of HS, as is intense pulsed light therapy (IPL) using the same principles of laser hair removal. There is weak evidence to recommend the use of carbon dioxide, diode or alexandrite lasers. The need for larger randomized controlled trials is highlighted. Copyright © 2016 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.

  6. Clinical experience of the use of adalimumab in the management of hidradenitis suppurativa. Comparison of response rates with Crohn disease.

    Science.gov (United States)

    Moyano, B; Clemente, A; Marín-Jiménez, I; Martorell, A

    2016-09-01

    The recent approval of adalimumab as the first treatment to be approved for the management of hidradenitis suppurativa has represented a before and after in the control of this chronic inflammatory disease. Given the inflammatory burden of this cutaneous disease, in the last few years hidradenitis suppurativa has been compared with inflammatory bowel disease, particularly with Crohn disease, to the point of considering hidradenitis suppurativa as "Crohn disease of the skin". These two chronic inflammatory diseases show sufficient similarities to consider whether treatment response based on the inflammatory load could also be similar. The present article aims to analyse the efficacy of adalimumab in hidradenitis suppurativa in comparison with a truly comparable disease, Crohn disease, with a view to evaluating therapeutic response rates and to drawing conclusions on the therapeutic success obtained in this disabling cutaneous disease.

  7. Is hidradenitis suppurativa associated with anaemia?: a population-based and hospital-based cross-sectional study from Denmark.

    Science.gov (United States)

    Miller, I M; Johansen, M E; Mogensen, U B; Zarchi, K; Ellervik, C; Jemec, G B

    2016-08-01

    Chronic inflammatory diseases may be associated with anaemia of inflammation. Hidradenitis suppurativa is a chronic inflammatory dermatological disease associated with metabolic comorbidities, low quality of life and fatigue. Anaemia may cause fatigue, and it has been hypothesized that HS-related fatigue may be partly due to anaemia. Our objective was to investigate a possible association between HS and anaemia. We performed a hospital-based and population-based cross-sectional study investigating the red blood cell profile, i.e. haemoglobin. We identified a total of 32 hospital HS individuals, 430 population HS individuals and 20,780 population non-HS control individuals. The age-sex-smoking-adjusted analyses showed no differences in the haemoglobin level of the HS groups vs. the control group. Analyses of the anaemic subgroup of HS individuals revealed that 60% had normocytic anaemia and 40% microcytic anaemia, in concordance with anaemia of inflammation. In contrast to our hypothesis, this study showed that HS is not associated with anaemia. Thus, anaemia may not be the cause of the described fatigue in HS patients. Furthermore, the results indicate that if an HS patient does suffer from anaemia it is most likely to be normocytic or microcytic and thus compatible with anaemia seen in other chronic inflammatory disorders. © 2015 European Academy of Dermatology and Venereology.

  8. Radical Surgical Excision and Use of Lateral Thoracic Flap for Intractable Axillary Hidradenitis Suppurativa

    Directory of Open Access Journals (Sweden)

    Wan-Lin Teo

    2012-11-01

    Full Text Available Current treatments for hidradenitis suppurativa (HS include prolonged courses of antibiotics,retinoids, immunosuppressants, and biologics. Severe cases that are resistant to prolongedmedical treatment pose a therapeutic challenge. We propose radical excision and lateral thoracicflap reconstruction as a treatment option for such cases. In our experience with two patients,good aesthetic and functional outcomes were achieved, with a high level of patient satisfaction.The availability of suitable flap coverage allows for wide resection of all of the hair-bearing skin,leading to a low incidence of residual disease and subsequent recurrence. Following excisionof the affected tissue, the ideal reconstructive method in the axilla provides suitable coveragewithout unacceptable donor site morbidity and also avoids axillary contractures. A long lateralthoracic flap with delay has excellent coverage with minimal donor tissue sacrifice. With asuitable flap coverage option, the management paradigm of intractable HS should shift fromprolonged medical treatment to allow decisive radical excision, which will improve the qualityof life for patients.

  9. Radical Surgical Excision and Use of Lateral Thoracic Flap for Intractable Axillary Hidradenitis Suppurativa

    Directory of Open Access Journals (Sweden)

    Wan-Lin Teo

    2012-11-01

    Full Text Available Current treatments for hidradenitis suppurativa (HS include prolonged courses of antibiotics, retinoids, immunosuppressants, and biologics. Severe cases that are resistant to prolonged medical treatment pose a therapeutic challenge. We propose radical excision and lateral thoracic flap reconstruction as a treatment option for such cases. In our experience with two patients, good aesthetic and functional outcomes were achieved, with a high level of patient satisfaction. The availability of suitable flap coverage allows for wide resection of all of the hair-bearing skin, leading to a low incidence of residual disease and subsequent recurrence. Following excision of the affected tissue, the ideal reconstructive method in the axilla provides suitable coverage without unacceptable donor site morbidity and also avoids axillary contractures. A long lateral thoracic flap with delay has excellent coverage with minimal donor tissue sacrifice. With a suitable flap coverage option, the management paradigm of intractable HS should shift from prolonged medical treatment to allow decisive radical excision, which will improve the quality of life for patients.

  10. Hidradenitis suppurativa: guidelines of the Italian Society of Dermatology and Venereology (SIDeMaST) for the use of anti-TNF-α agents.

    Science.gov (United States)

    Megna, M; Bettoli, V; Chimenti, S; Chiricozzi, A; Naldi, L; Virgili, A; Girolomoni, G; Monfrecola, G

    2015-12-01

    Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease characterized by nodules, abscesses and sinus tracts, primarily affecting the intertriginous areas. The occlusion of the upper part of the folliculopilosebaceous unit, leading to rupture of the sebofollicular canal with the consequent development of perifollicular lympho-histiocytic inflammation, is believed to be the initial pathogenic event in HS. Giving the chronic nature of HS, its destructive impact on social, working and daily life of patients, its management is often frustrating both for patients and physicians. The HS treatment choices are influenced by disease severity and its individual subjective impact. In this article, the Board of the Italian Society of Dermatology and Venereology (SIDeMaST) on HS has prepared a document focusing on the role of biologic drugs (anti-TNF-α) in HS management, providing also a flow-chart for HS handling and the inclusion and exclusion criteria for HS treatment with anti-TNF-α.

  11. The Clinical Significance of Increased Serum Proinflammatory Cytokines, C-Reactive Protein, and Erythrocyte Sedimentation Rate in Patients with Hidradenitis Suppurativa

    Directory of Open Access Journals (Sweden)

    D. Jiménez-Gallo

    2017-01-01

    Full Text Available Objectives. To assess inflammatory serum markers including serum proinflammatory cytokines, C-reactive protein (CRP, and erythrocyte sedimentation rate (ESR according to the clinical inflammatory activity of patients with hidradenitis suppurativa (HS. Patients and Methods. Seventy-four patients with HS were studied based on the Hidradenitis Suppurativa-Physician Global Assessment (HS-PGA score and Hurley staging system. Proinflammatory cytokines were measured using a multiplex cytokine assay. Twenty-two healthy volunteers were recruited. Results. Serum interleukin- (IL- 6, IL-23, soluble tumour necrosis factor alpha (TNF-α receptor I (sTNF-RI, CRP, and ESR were different in the patients with HS compared with those in the healthy controls (P0.25, P<0.05. The levels of IL-6 (r=0.53, P<0.001, CRP (r=0.54, P<0.001, and ESR (r=0.60, P<0.001 were especially well correlated with clinical inflammatory activity based on HS-PGA scores. The levels of IL-6, IL-8, sTNF-RI, sTNF-RII, CRP, and ESR were significantly elevated according to Hurley staging system. Conclusions. Serum proinflammatory cytokines, CRP, and ESR are increased in relation to the clinical inflammatory activity of patients with HS compared with healthy controls. Serum IL-6, CRP, and ESR are effective biomarkers for evaluating the severity of HS.

  12. Protocol for the development of a core domain set for hidradenitis suppurativa trial outcomes

    Science.gov (United States)

    Thorlacius, Linnea; Garg, Amit; Villumsen, Bente; Esmann, Solveig; Kirby, Joslyn S; Gottlieb, Alice B; Merola, Joseph F; Dellavalle, Robert; Jemec, Gregor B E

    2017-01-01

    Introduction Randomised controlled trials (RCTs) should have well-defined primary and secondary outcomes to answer questions generated by the main hypotheses. However, for the chronic, inflammatory skin disease hidradenitis suppurativa (HS), the reported outcome measures are numerous and diverse. A recent systematic review found a total of 30 outcome measure instruments in 12 RCTs. This use of a broad range of outcome measures can increase difficulties in interpretation and comparison of results and may potentially obstruct appropriate evidence synthesis by causing reporting bias. One strategy for dealing with these problems is to develop a core outcome set (COS). A COS is a list of outcomes that are meant as mandatory and should be measured and reported in all clinical trials. The aim of this study is to develop a COS for the management of HS. Method and analysis An international steering group of researchers, clinicians and a patient research partner will guide the COS development. 6 stakeholder groups are involved: patients, dermatologists, surgeons, nurses, industry representatives and drug regulatory authorities. A 1:1 ratio of patients:healthcare professionals is aimed for. The initial list of candidate items will be obtained by combining three data sets: (1) a systematic review of the literature, (2) US and Danish qualitative interview studies involving patients with HS and (3) an online healthcare professional (HCP) item generation survey. To reach consensus on the COS, 4 anonymous online Delphi rounds are then planned together with 2 face-to-face consensus meetings (1 in Europe and 1 in the USA) to ensure global representation. Ethics and dissemination The study will be performed according to the Helsinki declaration. All results from the study, including inconclusive or negative results, will be published in peer-reviewed indexed journals. The study will involve different stakeholder groups to ensure that the developed COS will be suitable and well

  13. Improvement of overlapping hidradenitis suppurativa and ankylosing spondylitis after the introduction of adalimumab

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    Dubravka Bosnić

    2016-12-01

    Full Text Available Hidradenitis suppurativa is a chronic inflammatory disorder characterized by occlusion of the follicular pilosebaceous units of the skin. The treatment options are sometimes very limited and unpleasant odor and abundant drainage complicate the disease. Ankylosing spondylitis is a form of seronegative spondyloarthritis with predominantly axial but also peripheral joint involvement. Both of the conditions lower the patient’s quality of life and affect everyday activities. We describe a 39-year-old male patient with both diseases treated with different medications with only a modest result. After the initiation of a tumor necrosis factor α (TNF-α inhibitor (adalimumab the patient experienced first the musculoskeletal and later on the skin improvement. The introduction of TNF- inhibitors should be considered early in the treatment of overlapping hidradenitis suppurativa and the spondyloarthritis spectrum of conditions. Available medical data confirm the positive results and beneficial effect on disease course, activity and, most importantly, quality of life.

  14. Improvement of overlapping hidradenitis suppurativa and ankylosing spondylitis after the introduction of adalimumab

    Science.gov (United States)

    Bosnić, Dubravka; Žarković, Branimir; Zarkovic, Maja; Anić, Branimir

    2016-01-01

    Hidradenitis suppurativa is a chronic inflammatory disorder characterized by occlusion of the follicular pilosebaceous units of the skin. The treatment options are sometimes very limited and unpleasant odor and abundant drainage complicate the disease. Ankylosing spondylitis is a form of seronegative spondyloarthritis with predominantly axial but also peripheral joint involvement. Both of the conditions lower the patient’s quality of life and affect everyday activities. We describe a 39-year-old male patient with both diseases treated with different medications with only a modest result. After the initiation of a tumor necrosis factor α (TNF-α) inhibitor (adalimumab) the patient experienced first the musculoskeletal and later on the skin improvement. The introduction of TNF-α inhibitors should be considered early in the treatment of overlapping hidradenitis suppurativa and the spondyloarthritis spectrum of conditions. Available medical data confirm the positive results and beneficial effect on disease course, activity and, most importantly, quality of life. PMID:28115784

  15. Improvement of overlapping hidradenitis suppurativa and ankylosing spondylitis after the introduction of adalimumab.

    Science.gov (United States)

    Bosnić, Dubravka; Žarković, Branimir; Barešić, Marko; Zarkovic, Maja; Anić, Branimir

    2016-01-01

    Hidradenitis suppurativa is a chronic inflammatory disorder characterized by occlusion of the follicular pilosebaceous units of the skin. The treatment options are sometimes very limited and unpleasant odor and abundant drainage complicate the disease. Ankylosing spondylitis is a form of seronegative spondyloarthritis with predominantly axial but also peripheral joint involvement. Both of the conditions lower the patient's quality of life and affect everyday activities. We describe a 39-year-old male patient with both diseases treated with different medications with only a modest result. After the initiation of a tumor necrosis factor α (TNF-α) inhibitor (adalimumab) the patient experienced first the musculoskeletal and later on the skin improvement. The introduction of TNF-α inhibitors should be considered early in the treatment of overlapping hidradenitis suppurativa and the spondyloarthritis spectrum of conditions. Available medical data confirm the positive results and beneficial effect on disease course, activity and, most importantly, quality of life.

  16. Hidradenitis suppurativa presenting with a posterior neck mass: a case report

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    Lee, Seung Young; Jeon, Min Hee; Bae, Il Heon; Han, Gi Seok; Cha, Sang Hoo; Kim, Sung Jin; Park, Kil Sun [Chungbuk National University Hospital, Cheongju (Korea, Republic of)

    2007-06-15

    Hidradenitis suppurativa is a rare disorder that is characterized by recurrent chronic skin infections and the formation of sinus tracts and considerable scaring. A 37-year-old man presented with a hard posterior neck mass. Multiple pus-producing sinuses were detected in the skin covering the mass. MRI demonstrated an ill-defined, soft tissue mass with multiple variable sized cystic lesions. The soft tissue mass measured 12 x 10 x 4 cm in the subcutaneous fat layer, it contained multifocal cystic lesions that revealed higher signal intensity on both the T1-and T2-weighted images, as compared with the adjacent neck muscles. The mass was not enhanced on the post-contrast T1 weighted images. Some of the cystic lesions extended to the skin. The mass was removed surgically and confirmed to be hidradenitis suppurativa.

  17. The clinical pattern of axillary hidradenitis suppurativa among Saudi Arabians: Mode of presentation and treatment challenges

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    Bader Hamza Shirah

    2017-01-01

    Full Text Available Background: Hidradenitis suppurativa is a chronic follicular occlusive disease affecting the folliculopilosebaceous unit. The clinical course is variable, ranging from mild to severe cases. Definitive evidence-based guidelines for the management are lacking. In addition, the multifaceted clinical features and the unpredictable course of the disease make a uniform approach to treatment impractical. As a result, there are multiple therapeutic approaches. Therefore, in this study, we aim to analyze the results of management of axillary hidradenitis suppurativa among the local community in Saudi Arabia. Materials and Methods: A retrospective cohort database analysis of 1369 patients diagnosed and treated for axillary hidradenitis suppurativa between January 2004 and December 2013 was done. Seven hundred and forty-one (54.12% were females and 628 (45.87% were males. All patients with Stage I disease favored the conservative method while all patients with Stage II chose the surgical approach. Results: The mean age was 25.5 years. Nine hundred and seventy-four (71.15% patients were treated conservatively; the mean healing time was 5.5 weeks. Three hundred and ninety-five (28.85% patients were treated surgically. One hundred and fourteen (28.9% had incision and drainage, 281 (71.1% had complete excision; all wounds eventually healed without significant scarring. The recurrence rate was 12.5% (122 patients in the conservative method group and zero (0% in the surgical method group. Conclusions: Hidradenitis suppurativa remains a challenging clinical condition for patients and physicians. Further studies need to focus not only on the etiology of this disease but also on the optimal treatment regimen. Public awareness programs are necessary to avoid late presentation and complications.

  18. Utility of transperineal and anal ultrasonography in the diagnostics of hidradenitis suppurativa and its differentiation from a rectal fistula.

    Science.gov (United States)

    Kołodziejczak, Małgorzata; Sudoł-Szopińska, Iwona; Wilczyńska, Aleksandra; Bierca, Jacek

    2012-11-14

    The pathogenesis of hidradenitis suppurativa (HS) is not fully understood. There exist several theories, in which mechanical factors, genetic factors, as well as immunological dysfunction of lymphocytes are suspected. Clinically, this entity is frequently mistaken for anal fistula with consequently wrong treatment. We aim to determine the utility of transperineal ultrasound (TPUS) and anal ultrasound (AUS) in the diagnosis of HS and its differentiation from an anal fistula. Retrospective analysis was performed on 51 patients (5 females, 46 males) aged 20-71 years (mean age 47.5), who were operated on in the years 2006-2011 for HS in the area of the anus and perineum, and pre-operatively had been imaged with TPUS and AUS. Sixty-seven operations were analyzed, as 11 patients were operated on more than once due to HS recurrence. In 66 out of 67 cases (98.5%), the pre-operative TPUS and AUS were in accordance with the intraoperative findings. Only in 1 patient was a pilonidal cyst diagnosed intraoperatively. In all 67 patients, the TPUS showed typical fluid-solid changes localized in the subcutaneous adipose tissue. In 6 out of 67 cases of HS (8.9%) AUS showed an anal fistula coexisting with the HS. In 2 cases (2.9%) a skin malignancy coexisting with HS was found. TPUS is an accessible imaging method, which confirms the typical localization of changes of HS, and together with AUS it allows for the proper differentiation of HS from an anal fistula or an abscess.

  19. Hidradenitis suppurativa: retrospective study of 20 cases Hidradenite supurativa: estudo retrospectivo de 20 casos

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    Nurimar Conceição Fernandes

    2013-06-01

    Full Text Available In this study, twenty cases of severe hidradenitis suppurativa are reported, mainly in non-white people and in axillary areas. Wide surgical excision has offered good results, although relapses have occurred at variable intervals in the follow-up period.São relatados 20 casos de hidradenite supurativa grave, com predomínio de não brancos e regiões axilares. A excisão cirúrgica ampla trouxe bons resultados, embora a recidiva ocorresse em intervalos variáveis no acompanhamento.

  20. Bacterial Colonization in Hidradenitis Suppurativa/Acne Inversa: A Cross-sectional Study of 50 Patients and Review of the Literature.

    Science.gov (United States)

    Nikolakis, Georgios; Liakou, Aikaterini I; Bonovas, Stefanos; Seltmann, Holger; Bonitsis, Nikolaos; Join-Lambert, Olivier; Wild, Thomas; Karagiannidis, Ioannis; Zolke-Fischer, Silvia; Langner, Klaus; Zouboulis, Christos C

    2016-11-24

    It is unclear whether bacterial colonization in hidradenitis suppurativa/acne inversa (HS) comprises a primary cause, triggering factor or secondary phenomenon of the disease pathogenesis. Furthermore, the connection between certain bacterial species, the disease severity and its localization is unknown. Bacterial species were isolated from HS lesions to reveal a potential correlation with localization and disease severity. Ninety swab tests were prospectively obtained from 90 HS lesions of 50 consecutive patients. The material was cultured under aerobic and anaerobic conditions. The identified species were statistically correlated with Hurley stage and localization of the lesions. The most prevalent isolates were reported. Hurley stage significantly correlated with disease localization. Particular bacterial species were associated with "extended" disease and Hurley III stage with the detection of both aerobic and anaerobic bacteria and with a higher number of species. The presence of bacterial species is dependent on the local milieu, which correlates with the localization of the disease, its clinical manifestations and its extension.

  1. Topical 5-aminolevulinic acid photodynamic therapy improved refractory acne conglobata and perifolliculitis capitis abscedens et suffodiens rather than hidradenitis suppurativa

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    Linglin Zhang

    2016-01-01

    Full Text Available Acne conglobata (AC, perifolliculitis capitis abscedens et suffodiens (PCAS and hidradenitis suppurativa (HS are uncommon refractory chronic, inflammatory, scarring diseases but cause serious damage to the quality of life. These three diseases are associated with follicular occlusion. Several studies indicated topical 5-aminolevulinic acid photodynamic therapy (ALA-PDT improved follicular occlusion besides acne treatment. So we attempted to apply ALA-PDT to medicine resistant AC, PCAS and HS. Topical ALA-PDT was applied to 10 patients with AC, seven patients with PCAS and three patients with HS for more than three sessions. All the patients completed the dermatology life quality index (DLQI questionnaire and were assessed for the efficacy at the baseline and on two weeks after each treatment. Adverse effects were recorded at each visit. The results showed 25.5% (5/20, two cases of AC and three cases of PCAS of patients achieved excellent improvement after three sessions of PDT and another 60.0% (12/20, eight cases of AC and four cases of PCAS of patients achieved good improvement. 15.0% (3/20, three cases of HS got poor response (< 20% lesions clearance. Another five cases (three cases of AC and two cases of PCAS also achieved excellent response after 5–7 sessions of PDT. We also found that papular/nodular, cyst/abscess showed higher clearance rate than sinus/fistula (88.5%, 86.1% versus 11.1%. DLQI was reduced after three sessions of PDT in AC and PCAS patients rather than HS patients. 5-ALA-PDT could improve refractory AC and PCAS but could not lead to improvement in late stage of HS. The efficacy increased with more treatment sessions.

  2. A Disease-Modifying Approach for Advanced Hidradenitis Suppurativa (Regimen with Metformin, Liraglutide, Dapsone, and Finasteride: A Case Report

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    Birgit N. Khandalavala

    2017-07-01

    Full Text Available Hidradenitis suppurativa (HS is a challenging skin disease with limited therapeutic options. Obesity and metabolic syndrome are being increasingly implicated and associated with younger ages and greater metabolic severity. A 19-year-old female with an 8-year history of progressively debilitating cicatricial HS disease presented with obesity, profound anemia, leukocytosis, increased platelet count, hypoalbuminemia, and elevated liver enzymes. A combination of metformin, liraglutide, levonorgestrel-ethinyl estradiol, dapsone, and finasteride was initiated. Acute antibiotic use for recurrences and flares could be slowly discontinued. Over the course of 3 years on this regimen, the liver enzymes normalized in 1 year, followed in2 years by complete resolution of the majority of the hematological and metabolic abnormalities. The sedimentation rate reduced from over 120 to 34 mm/h. She required 1 surgical intervention for perianal disease after 9 months on the regimen. Flares greatly diminished in intensity and duration, with none in the past 6 months. Right axillary lesions have completely healed with residual disease greatly reduced. Chiefly abdominal lesions are persistent. She was able to complete high school from home, start a job, and resume a normal life. Initial weight loss of 40 pounds was not maintained. The current regimen is being well tolerated and continued.

  3. A prospective randomized controlled trial assessing the efficacy of adjunctive hyperbaric oxygen therapy in the treatment of hidradenitis suppurativa.

    Science.gov (United States)

    Yildiz, Hamza; Senol, Levent; Ercan, Erdinc; Bilgili, Memet Ersan; Karabudak Abuaf, Ozlem

    2016-02-01

    Hyperbaric oxygen therapy (HBOT) appears to enhance wound healing, increase bactericidal activity, and act synergistically with a number of antibiotics. The aim of this study was to evaluate the efficacy of HBOT as an adjunctive therapy in patients with hidradenitis suppurativa (HS) treated with a combination of systemic rifampicin and clindamycin. The study was a prospective, single-center, single-dose, open-label, randomized controlled clinical study of HBOT in patients with moderate to severe HS. Efficacy was measured by modified Sartorius score (SS), HS Severity Index (HSSI), Dermatology Life Quality Index (DLQI), and a visual analog scale (VAS) before treatment and after the completion of 4 and 10 weeks of treatment. Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) levels were also measured. Forty-three patients were enrolled in the study. More patients in the HBOT than in the control group showed a decrease of ≥50% from baseline parameters at week 10 for SS (100%), HSSI (100%), DLQI (95.5%), VAS (100%), ESR (100%), and CRP (72.7%). Clinically and statistically significant improvements from baseline were observed at 4 and 10 weeks in HSSI (P = 0.009 at both), SS (P = 0.021 at both), and DLQI (P = 0.044 at week 4, P = 0.009 at week 10). Adjunctive HBOT was considered to be effective in significantly improving antibiotic treatment of HS. The treatment was well tolerated, and no unexpected safety issues were identified.

  4. Acne Conglobata Associated with Hidradenitis Suppurativa, Disorders of Follicular Occlusion (Case Report

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    Pecova K.

    2015-09-01

    Full Text Available The author is presenting the case of a 23-year-old female patient with a severe form of acne conglobata, with the first symptoms of the disease occurring as far back as the prepubertal age. In the past year the disease has combined with hidradenitis suppurativa (to be referred to henceforth as “HS”, Hurley stage I, in the axillae and both sides of the inguinal region, with a family history of acne conglobata (both her mother and brother were affected. Further examinations ruled out inflammatory bowel disease because of a lack of further associated symptoms, except for sideropenic anaemia (lesser form and lower serum values of vitamin D. Up until now the disease has been resistant to treatment, including the long-term treatment of methylprednisolone in combination with isotretinoid as well as dapsone and antibiotics.

  5. A study on the management of hidradenitis suppurativa with retinoids and surgical excision

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    Neerja Puri

    2011-01-01

    Full Text Available Background: Hidradenitis suppurativa is a chronic skin condition involving the apocrine glandular zones. Affected patients may present with acute abscesses, but the condition often progresses to a chronic state with persistent pain, sepsis, sinus tract, fistula formation, purulent discharge, and dermal scarring. The treatment of patients with severe disease can be difficult and may require complex surgical intervention. Materials and Methods: For this study, we selected 30 patients from the outpatient department. The patients were divided into two groups of 15 patients each. In patients of group I, oral acitretin 0.5 mg/kg body weight was given alone. Oral acitretin was given for a period of 12 weeks, and follow-up of the patients was done every 4 weeks for a period of 6 months. In patients of group II, oral acitretin 0.5 mg/kg was given plus a wide surgical excision was done. Results and Discussion: In our study, the commonest site of involvement of hidradenitis suppurativa was axilla in 83.3% patients, perineum was involved in 13.3% patients, and periumbilical involvement was seen in 3.3% patients. The commonest clinical feature was nodules seen in 90% patients; pain was seen in 60% patients, dermal scarring in 73.3% patients, malodorous discharge in 33.3% patients, abscess in 30% patients, and fistulous tracts were seen in 20% patients. The recurrence rate was low (20% in group II patients in whom oral acitretin was given plus surgical excision was done as compared with group I (40% in whom oral acitretin was given alone.

  6. Utility of transperineal and anal ultrasonography in the diagnostics of hidradenitis suppurativa and its differentiation from a rectal fistula 

    Directory of Open Access Journals (Sweden)

    Małgorzata Kołodziejczak

    2012-11-01

    Full Text Available Introduction:The pathogenesis of hidradenitis suppurativa (HS is not fully understood. There exist several theories, in which mechanical factors, genetic factors, as well as immunological dysfunction of lymphocytes are suspected. Clinically, this entity is frequently mistaken for anal fistula with consequently wrong treatment.We aim to determine the utility of transperineal ultrasound (TPUS and anal ultrasound (AUS in the diagnosis of HS and its differentiation from an anal fistula.Material/Methods:Retrospective analysis was performed on 51 patients (5 females, 46 males aged 20–71 years (mean age 47.5, who were operated on in the years 2006–2011 for HS in the area of the anus and perineum, and pre-operatively had been imaged with TPUS and AUS. Sixty-seven operations were analyzed, as 11 patients were operated on more than once due to HS recurrence.Results:In 66 out of 67 cases (98.5� the pre-operative TPUS and AUS were in accordance with the intraoperative findings. Only in 1 patient was a pilonidal cyst diagnosed intraoperatively. In all 67 patients, the TPUS showed typical fluid-solid changes localized in the subcutaneous adipose tissue. In 6 out of 67 cases of HS (8.9�20AUS showed an anal fistula coexisting with the HS. In 2 cases (2.9�20a skin malignancy coexisting with HS was found.Discussion:TPUS is an accessible imaging method, which confirms the typical localization of changes of HS, and together with AUS it allows for the proper differentiation of HS from an anal fistula or an abscess.

  7. Use of lasers for the management of refractory cases of hidradenitis suppurativa and pilonidal sinus

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    Vivek Jain

    2012-01-01

    Full Text Available Background: Hidradinitis suppurativa (HS and pilonidal sinus (PNS are chronic inflamatory skin diseases, often refractory to treatment and search for a new treatment is on. We tried deroofing with the help of carbon dioxide laser in patients of HS and PNS, however there was recurrence. Aim: To evaluate a technique combining the use of CO 2 laser and long pulse 1064 nm Neodymium-doped Yttrium Aluminium Garnet (Nd:YAG laser for the treatment of HS and PNS. Materials and Methods: In 4 patients with HS and 5 patients with PNS, we performed procedure in two steps: first destroying the hair follicles with long pulse Nd yag 1064 laserfollowed by deroofing with carbon di oxide laser. Follow up was done upto 3 years. Results: All patients with HS were females in the age group of 30-40 years. In PNS, 2 male patients were of age less than 20, two male patients of age more than 20 and one females of age less than 20. None of the HS or PNS patients showed recurrence. Conclusion: The deroofing with CO 2 laser along with hair follicle removal with long pulse Nd:YAG laser is an effective minimally invasive tissue saving surgical intervention for the treatment of refractory HS and PNS lesions.

  8. Hidradenitis Suppurativa

    Science.gov (United States)

    ... Boards study tools Online Learning Center Meetings and events Make a difference Career planning Media Relations Toolkit AAD apps Academy meeting Chronic urticaria—for members Chronic urticaria—for public Dermatology World Dialogues in Dermatology JAAD Mohs AUC ...

  9. Hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Jemec, G.B.E.

    2012-01-01

    A 36-year-old woman has recurrent boils under both arms and in the groin. They flare premenstrually, causing pain, suppuration, and an offensive odor. Scarring has developed in the groin area, and chronically draining sinus tracts are interspersed with normal skin. Treatment with short courses of...

  10. Should hidradenitis suppurativa/acne inversa best be renamed as "dissecting terminal hair folliculitis"?

    Science.gov (United States)

    Chen, WenChieh; Plewig, Gerd

    2016-09-13

    Hidradenitis suppurativa/acne inversa is a diverse, enigmatic and distressful disease that has aroused growing interest in specialists from different disciplines. Both names describe its classical manifestations in the intertriginous regions and reflect the historical view of the disease definition, but cause confusions in the understanding of its pathogenesis and classification. In the light of the advance in clinical, histopathological and pathophysiological findings, we propose the term "dissecting terminal hair folliculitis" (DTHF) to characterize its disease nature as folliculitis instead of acneiform disease or apocrine gland disorder. DTHF attacks exclusively the terminal hair follicles in an overwhelming majority of adults, initiating from the fragile acroinfundibulum leading to a non-infectious overreaction of innate immunity system with inflammation that may fiercely dissect and engulf all the surrounding tissues accompanied by secondary bacterial infections. Evidence indicates that perifolliculitis capitis abscedens et suffodiens and pilonidal disease are very likely regional variants of DTHF with the same pathogenesis. Treatment of DTHF remains frustrating. The benefit of biologics in targeting inflammation is so far non-specific, palliative and inconsistent. Hair epilation and photodynamic therapy in treatment of the disease is questionable in consideration of the pathogenesis. Genetic and translational research, especially on the Notch signalling pathways, will yield breakthrough in the development of novel treatment modalities.

  11. Reconstruction in extensive axillary Hidradenitis suppurativa with local fasciocutaneous V-Y advancement flaps

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    Sharma Ramesh

    2006-01-01

    Full Text Available We present our experience with the use of local fasciocutaneous V-Y advancement flaps in the reconstruction of 10 axillae in 6 patients for large defects following wide excision of long-standing Hidradenitis suppurativa of the axilla. The defects were closed with local V-Y subcutaneous island flaps. A single flap from the chest wall was sufficient for moderate defects. However, for larger defects, an additional flap was taken from the medial side of the ipsilateral arm. The donor defects could be closed primarily in all the patients. The local areas of the lateral chest wall and the medial side of the arm have a plentiful supply of cutaneous perforators and the flaps can be designed in a V-Y fashion without resorting to preoperative marking of the perforator. The flaps were freed sufficiently to allow adequate movement for closure of the defects. Although no attempt was made to identify the perforators specifically, many perforators were seen entering the flap. Some perforators can be safely divided to increase reach of the flap. All the flaps survived completely. A follow up of 2.5 years is presented.

  12. An interesting uncommon side effect of topical corticosteroids-hidradenitis suppurativa

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    Yugandar Inakanti

    2015-07-01

    Full Text Available There are many local and systemic side-effects of topical corticosteroids. To the best of our Knowledge, Hidradenitis Supportive (HS due to topical corticosteroids has not been reported previously. We report a 39-year old, male patient who started himself a topical corticosteroid Pander Plus cream because of itching, scaly lesions over groins and buttocks since 6 months. After 4 months of topical treatment, he developed pus discharging sinuses over both groins and buttocks. Based on patient history, clinical and laboratory findings and the exclusion of other diagnoses, HS points out the use of topical corticosteroids. The case well highlights this unusual condition and represents the first case reported in India to our best of the knowledge. Having performed thorough literature search I would like to discuss in this report the evidence for this relation and stress the importance of appropriate usage of topical corticosteroids.

  13. Risk factors for hidradenitis suppurativa: a pilot study Fatores de risco para hidradenite supurativa: um estudo piloto

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    Juliano Vilaverde Schmitt

    2012-12-01

    Full Text Available The hidradenitis suppurativa is a chronic debilitating inflammatory disease whose etiology is not fully understood. We conducted a pilot case-control study matched by sex and age with other dermatological patients to analyze possible risk factors associated with this disease. We included 15 cases and 45 controls, 67% were women. Bivariate and multivariate logistic regression analysis identified significant association with smoking, higher body mass index and family history. The use of hormonal contraceptives was less frequent in women with hidradenitis.A hidradenite supurativa é uma doença inflamatória crônica debilitante de etiologia parcialmente compreendida. Realizamos um estudo piloto tipo caso-controle pareado por sexo e idade com outros pacientes dermatológicos para analisar prováveis fatores de risco associados a esta doença. Incluímos 15 casos e 45 controles, sendo 67% mulheres. Análise bivariada e multivariada por regressão logística identificou associação significativa com tabagismo, índice de massa corporal mais elevado e história familiar. O uso de contraceptivos hormonais foi menos frequente nas portadoras de hidradenite.

  14. Hidradenitis suppurativa: a disease of the absent sebaceous gland? Sebaceous gland number and volume are significantly reduced in uninvolved hair follicles from patients with hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Kamp, S; Fiehn, A M; Dokkedahl, Karin Stenderup;

    2011-01-01

    in paraffin and stained with haematoxylin and eosin prior to volume estimation using the Cavalieri principle. RESULTS: Sebaceous gland tissue could be visualized in only 10 of 15 suitable hair follicle biopsies from patients with HS but was present in all biopsies from healthy controls (P = 0·05) and the mean...

  15. A Population- and Hospital-based Cross-sectional Study of Renal Function in Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Miller, Iben M; Carlson, Nicholas; Mogensen, Ulla Brasch;

    2016-01-01

    similar basement membrane defects might occur in renal tissue. Our objective was to investigate a possible association between HS and renal dysfunction. We performed a hospital and population-based cross-sectional study using estimated Glomerular-Filtration-Rate (eGFR) to assess renal function. Thirty......-two hospital individuals with HS, 430 population individuals with HS, and 20, 780 population individuals without HS were identified. The age-sex-smoking-BMI-hypertension-diabetes adjusted analysis revealed a statistically significant higher eGFR for the hospital group with HS and a mean difference in eGFR of 6.......81 (1.27-12.35) ml/min/1.73 m between the hospital group with HS and the population group without HS. The observed higher eGFR in the hospital group with HS indicates a possible association of HS and renal dysfunction....

  16. Topical treatments of skin pain: a general review with a focus on hidradenitis suppurativa with topical agents.

    Science.gov (United States)

    Scheinfeld, Noah

    2014-07-15

    Hidradenitis Supprurativa (HS) is a painful chronic follicular disease. Few papers have addressed pain control for this debilitating condition. Possible topical agents include tricyclic antidepressants, opioids, anticonvulsants, NSAIDs, NMDA receptor antagonists, local anesthetics and other agents. The first line agents for the topical treatment of the cutaneous pain of HS are diclonefac gel 1% and liposomal xylocaine 4% and 5% cream or 5% ointment. The chief advantage of topical xylocaine is that is quick acting i.e. immediate however with a limited duration of effect 1-2 hours. The use of topical ketamine, which blocks n-methyl-D-aspartate receptors in a non-competitive fashion, might be a useful tool for the treatment of HS pain. Topical doxepin, which available in a 5% commercially preparation (Zonalon®) , makes patients drowsy and is not useful for controlling the pain of HS . Doxepin is available in a 3% or 3.3% concentration (which causes less drowsiness) from compounding pharmacies and can be used in compounded analgesic preparations with positive effect. Topical doxepin is preferred over use of topical amitriptyline because topical doxepin is more effective. Nevertheless, topical amitriptyline increase of the tactile and mechanical nociceptive thresholds and can be used for topical pain control in compound mixture of analgesics . Gabapentin and pregablin can also be used compounded with other agents in topical analgesic preparations with positive topical anesthetic effect. Capsaicin is not useful for topical treatment of the pain of HS. Sometimes compounded of anesthetics medications such as ketamine 10%, bupivacaine 1%, diclofenac 3%, doxepin 3% or 3.3%, and gabapentin 6% can extend the duration of effect so that medication only needs to be used 2 or 3 times a day. Still in my experience the easiest to get and most patient requested agent is topical diclonefac 1% gel.

  17. Normal Skin Microbiota is Altered in Pre-clinical Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Ring, Hans Christian; Bay, Lene; Kallenbach, Klaus

    2017-01-01

    therefore hypothesized that clinically unaffected HS skin would also have an increased presence of biofilm compared with that of healthy controls. We conducted a case-control study, investigating the morphology of the axillary skin microbiota. Peptide nucleic acid – fluorescence in situ hybridization probes...... were used in combination with confocal laser scanning microscopy. Significant differences were found in both distribution and quantity of the cutaneous microbiota in clinically non-affected axillary skin of patients with HS compared with healthy controls. Surprisingly, we detected fewer bacteria...... and less biofilm in patients with HS. The reduced microbiota in patients with HS may play an important role in the early course of the disease....

  18. Pyoderma gangrenosum, acne, suppurative hidradenitis (PASH) and polycystic ovary syndrome: Coincidentally or aetiologically connected?

    Science.gov (United States)

    Zivanovic, Dubravka; Masirevic, Iva; Ruzicka, Thomas; Braun-Falco, Markus; Nikolic, Milos

    2016-02-02

    The clinical triad of pyoderma gangrenosum, acne conglobata and hidradenitis suppurativa has been named PASH syndrome. Polycystic ovary syndrome (PCOS) is associated with hyperandrogenism and inflammation. Hidradenitis suppurativa, like acne vulgaris, may be a feature of hyperandrogenism. Obesity may be associated with both hidradenitis suppurativa and PCOS. We describe a possible association between PASH syndrome and PCOS.

  19. Experience with ustekinumab for the treatment of moderate to severe Hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Gulliver, W.P.; Baker, K.A.; Jemec, G.B.E.

    2012-01-01

    , however, abrogation of tumour necrosis factor-α (TNF-α) function has proven effective in some patients. Objective To assess the safety and efficacy of the interleukin-12/23 inhibitor, ustekinumab for treatment of HS in three patients with moderate-severe disease. Methods The subjects received 3-45 mg...... subcutaneous injections of ustekinumab at 0, 1 and 4 months. Improvement was assessed by the dermatology life quality index (DLQI), visual analogue scale of pain (VAS) and physician's global assessment (PGA) at each monthly visit. Results Prior to treatment, subjects had moderate-severe HS (Hurley stage II......-III) with a DLQI score between 8 and 12. At 6 months, one patient showed complete disease remission, while a 25-49% improvement was seen in a second patient and no change in a third. A moderate but statistically significant relationship was observed between VAS and DLQI scores (r = 0.75; P

  20. Protocol for the development of a core domain set for hidradenitis suppurativa trial outcomes

    DEFF Research Database (Denmark)

    Thorlacius, Linnea; Ingram, John R; Garg, Amit

    2017-01-01

    Delphi rounds are then planned together with 2 face-to-face consensus meetings (1 in Europe and 1 in the USA) to ensure global representation. ETHICS AND DISSEMINATION: The study will be performed according to the Helsinki declaration. All results from the study, including inconclusive or negative...... for dealing with these problems is to develop a core outcome set (COS). A COS is a list of outcomes that are meant as mandatory and should be measured and reported in all clinical trials. The aim of this study is to develop a COS for the management of HS. METHOD AND ANALYSIS: An international steering group...... of researchers, clinicians and a patient research partner will guide the COS development. 6 stakeholder groups are involved: patients, dermatologists, surgeons, nurses, industry representatives and drug regulatory authorities. A 1:1 ratio of patients:healthcare professionals is aimed for. The initial list...

  1. Remission of refractory pyoderma gangrenosum, severe acne, and hidradenitis suppurativa (PASH) syndrome using targeted antibiotic therapy in 4 patients.

    Science.gov (United States)

    Join-Lambert, Olivier; Duchatelet, Sabine; Delage, Maïa; Miskinyte, Snaigune; Coignard, Hélène; Lemarchand, Nicolas; Alemy-Carreau, Murielle; Lortholary, Olivier; Nassif, Xavier; Hovnanian, Alain; Nassif, Aude

    2015-11-01

    Pyoderma gangrenosum, severe acne, and suppurative hidradenitis (PASH) syndrome can prove refractory to treatment and is characterized by relapses and recurrences. The combination of antibiotic therapy and surgery can produce success in the management of the syndrome. Acute treatment is required, but maintenance therapy is also necessary to prevent disease relapse. The response to antibiotic therapy is hypothesis generating, raising the issue of a modified host response. To date, anecdotal reports support the use of surgery and medical therapy, but controlled investigations with extended follow-up are necessary to substantiate preliminary data observed with individual cases.

  2. Dissecting cellulitis (Perifolliculitis Capitis Abscedens et Suffodiens): a comprehensive review focusing on new treatments and findings of the last decade with commentary comparing the therapies and causes of dissecting cellulitis to hidradenitis suppurativa.

    Science.gov (United States)

    Scheinfeld, Noah

    2014-05-16

    Dissecting cellulitis (DC) also referred to as to as perifolliculitis capitis abscedens et suffodiens (Hoffman) manifests with perifollicular pustules, nodules, abscesses and sinuses that evolve into scarring alopecia. In the U.S., it predominantly occurs in African American men between 20-40 years of age. DC also occurs in other races and women more rarely. DC has been reported worldwide. Older therapies reported effective include: low dose oral zinc, isotretinoin, minocycline, sulfa drugs, tetracycline, prednisone, intralesional triamcinolone, incision and drainage, dapsone, antiandrogens (in women), topical clindamycin, topical isotretinoin, X-ray epilation and ablation, ablative C02 lasers, hair removal lasers (800nm and 694nm), and surgical excision. Newer treatments reported include tumor necrosis factor blockers (TNFB), quinolones, macrolide antibiotics, rifampin, alitretinoin, metronidazole, and high dose zinc sulphate (135-220 mg TID). Isotretinoin seems to provide the best chance at remission, but the number of reports is small, dosing schedules variable, and the long term follow up beyond a year is negligible; treatment failures have been reported. TNFB can succeed when isotretinoin fails, either as monotherapy, or as a bridge to aggressive surgical treatment, but long term data is lacking. Non-medical therapies noted in the last decade include: the 1064 nm laser, ALA-PDT, and modern external beam radiation therapy. Studies that span more than 1 year are lacking. Newer pathologic hair findings include: pigmented casts, black dots, and "3D" yellow dots. Newer associations include: keratitis-ichthyosis-deafness syndrome, Crohn disease and pyoderma gangrenosum. Older associations include arthritis and keratitis. DC is likely a reaction pattern, as is shown by its varied therapeutic successes and failures. The etiology of DC remains enigmatic and DC is distinct from hidradenitis suppurativa, which is shown by their varied responses to therapies and their

  3. A double-blind placebo-controlled randomized trial of adalimumab in the treatment of hidradenitis suppurativa

    DEFF Research Database (Denmark)

    Miller, I; Lynggaard, C D; Lophaven, S

    2011-01-01

    subcutaneously (s.c.) at baseline followed by 40 mg s.c. every other week for 12 weeks. Placebo-treated patients received identical-looking injections with no active ingredient. The medicine was dispensed in sequentially numbered computer-randomized containers. Participants, care givers and those assessing...... the outcomes were blinded to group assignment. The primary efficacy endpoints were changes in the HS scores (Sartorius and Hurley scoring systems). Secondary efficacy endpoints included changes in pain (visual analogue scale), days with lesions and Dermatology Life Quality Index, and evaluation of scarring...

  4. The ABC of Hidradenitis Suppurativa

    DEFF Research Database (Denmark)

    Lipsker, Dan; Severac, François; Freysz, Marie

    2016-01-01

    clinical situations were identified in which naming with established semantics is so far not possible. For these situations, the terms 'multicord', 'multipore', 'multitunnel' and 'retraction' were defined. DISCUSSION: This is the first validation of clinical terms used to describe lesions in patients...

  5. Steatocystoma multiplex suppurativa: case report of a rare condition*

    Science.gov (United States)

    Santana, Cândida Naira Lima e Lima; Pereira, Daniele do Nascimento; Lisboa, Alice Paixão; Leal, Juliana Martins; Obadia, Daniel Lago; da Silva, Roberto Souto

    2016-01-01

    Steatocystoma multiplex is a rare genetic disorder characterized by the presence of hamartomatous malformations at the junction of the pilosebaceous duct. It consists of encapsulated cystic lesions in the dermis, with adjacent sebaceous gland. When associated with inflammation, resembling hidradenitis, it is called steatocystoma multiplex suppurativa, a condition rarely reported. This is the first case of steatocystoma multiplex suppurativa reported in the Brazilian literature. Female patient, 23 years old, with papular and nodular cystic lesions that started in the armpits and groin, later spreading to the trunk, lower limbs, anticubital fossa, face and scalp. The presence of papular-nodular lesions associated with disseminated hidradenitis-like lesions in flexural areas and the histopathological diagnosis of steatocystoma defined the diagnosis of steatocystoma multiplex suppurativa.

  6. Hydradenitis suppurativa and inflammatory bowel disease: An unusual, but existing association.

    Science.gov (United States)

    Principi, Mariabeatrice; Cassano, Nicoletta; Contaldo, Antonella; Iannone, Andrea; Losurdo, Giuseppe; Barone, Michele; Mastrolonardo, Mario; Vena, Gino Antonio; Ierardi, Enzo; Di Leo, Alfredo

    2016-05-28

    Inflammatory bowel disease (IBD) could be associated with several extra-intestinal manifestations (EIMs) involving musculoskeletal, hepatopancreatobiliary, ocular, renal, and pulmonary systems, as well as the skin. In the last years, hidradenitis suppurativa (HS) is acquiring an increasing interest. IBD, especially Crohn's disease (CD), is among the most reported associated diseases in HS patients. The aim of this paper is to give a brief overview of data showing a possible epidemiologic and pathogenetic association between IBD and HS. We performed a pooled-data analysis of four studies and pooled prevalence of HS in IBD patients was 12.8%, with a 95%CI of 11.7%-13.9%. HS was present in 17.3% of subjects with CD (95%CI: 15.5%-19.1%) and in 8.5% of UC patients (95%CI: 7.0%-9.9%). Some items, especially altered immune imbalance, are generally involved in IBD pathogenesis as well as invoked by HS. Smoking is one of the most relevant risk factors for both disorders, representing a predictor of their severity, despite, actually, there being a lack of studies analyzing a possible shared pathway. A role for inheritance in HS and CD pathogenesis has been supposed. Despite a genetic susceptibility having been demonstrated for both diseases, further studies are needed to investigate a genetic mutual route. Although the pathogenesis of IBD and HS is generally linked to alterations of the immune response, recent findings suggest a role for intestinal and skin microbiota, respectively. In detail, the frequent finding of Staphylococcus aureus and coagulase-negative staphylococci on HS cutaneous lesions suggests a bacterial involvement in disease pathogenesis. Moreover, microflora varies in the different cutaneous regions of the body and, consequently, two different profiles of HS patients have been identified on these bases. On the other hand, it is well-known that intestinal microbiota may be considered as "the explosive mixture" at the origin of IBD despite the exact

  7. Acne inversa (Hidradenitis suppurativa: A review with a focus on pathogenesis and treatment

    Directory of Open Access Journals (Sweden)

    Uwe Wollina

    2013-01-01

    Full Text Available Acne inversa (AI is a disabilitating chronic inflammatory disease with major negative impact on quality of life and significant co-morbidities. This is an important link to insights into immune dysfunction, which stimulated therapeutic approaches like tumor necrosis-α inhibitor therapy. This new off-label drug treatment is particularly beneficial when used in combination with wide excision of inflamed skin and subcutaneous tissue. Retinoids have been reported to be helpful in secondary prevention. The standard of therapy in advanced cases is surgery with wide excisions and healing by secondary intention. This treatment results in significant reduction of complaints and achieves satisfactory body contouring.

  8. hs-CRP Test

    Science.gov (United States)

    ... be limited. Home Visit Global Sites Search Help? hs-CRP Share this page: Was this page helpful? Also ... it used? A high-sensitivity C-reactive protein (hs-CRP) test may be used to help evaluate an ...

  9. HS3 Data Catalog

    Science.gov (United States)

    Emory, Amber Elizabeth; Chirica, Dan Cristian; Doyle, Jim

    2013-01-01

    This presentation covered the original plan for the Hurricane and Severe Storm Sentinel (HS3) Data Catalog available through the ESPO HS3 mission page (http://espo.nasa.gov/missions/hs3/) and provided examples of Model Products, Operational Products, and Research (Instrument) Products from the 2012 field campaign. The presentation also covered lessons learned and suggested improvements to the Data Catalog for the upcoming 2013 HS3 field campaign.

  10. SN 2011hs

    DEFF Research Database (Denmark)

    Bufano, F.; Pignata, G.; Bersten, M.

    2014-01-01

    Observations spanning a large wavelength range, from X-ray to radio, of the Type IIb supernova 2011hs are presented, covering its evolution during the first year after explosion. The optical light curve presents a narrower shape and a fainter luminosity at peak than previously observed for Type I...

  11. Pyoderma gangrenosum, acne conglobata, suppurative hidradenitis, and axial spondyloarthritis: efficacy of anti-tumor necrosis factor α therapy.

    Science.gov (United States)

    Bruzzese, Vincenzo

    2012-12-01

    We report the case of a patient with a simultaneous presence of pyoderma gangrenosum, acne conglobata, suppurative hidradenitis, and axial spondyloarthritis. This condition differs from both the PASH (pyoderma gangrenosum, acne, and suppurative hidradenitis) syndrome, in which arthritis is absent, and the PAPA (pyogenic arthritis, pyoderma gangrenosum, and acne) syndrome, in which suppurative hidradenitis is lacking. Our patient failed to respond to etanercept therapy, whereas all dermatologic and rheumatic manifestations completely regressed following infliximab infusion. We therefore propose that simultaneous presence of pyoderma gangrenosum, acne conglobata, suppurative hidradenitis, and seronegative spondyloarthritis might represent a distinct syndrome that could be termed the PASS syndrome. Tumor necrosis factor α therapies seem to play selective roles.

  12. HS0139+0559, HS0229+8016, HS0506+7725 and HS0642+5049: Four new long-period cataclysmic variables

    CERN Document Server

    Aungwerojwit, A; Rodríguez-Gil, P; Hagen, H J; Harlaftis, E T; Papadimitriou, C; Lehto, H; Araújo-Betancor, S; Heber, U; Fried, R E; Engels, D; Katajainen, S

    2005-01-01

    We present time-resolved optical spectroscopy and photometry of four relatively bright (V~14.0-15.5) long-period cataclysmic variables (CVs) discovered in the Hamburg Quasar Survey: HS0139+0559, HS0229+8016, HS0506+7725 and HS0642+5049. Their respective orbital periods, 243.69+-0.49min, 232.550+-0.049min, 212.7+-0.2min and 225.90+-0.23min are determined from radial velocity and photometric variability studies. HS0506+7725 is characterised by strong Balmer and He emission lines, short-period (~10-20min) flickering and weak X-ray emission in the ROSAT All Sky Survey. The detection of a deep low state (~18.5) identifies HS0506+7725 as a member of the VY Scl stars. HS0139+0559, HS0229+8016 and HS0642+5049 display thick-disc like spectra and no or only weak flickering activity. HS0139+0559 and HS0229+8016 exhibit clean quasi-sinusoidal radial velocity varations of their emission lines but no or very little orbital photometric variability. In contrast, we detect no radial velocity variation in HS0642+5049 but a not...

  13. Italian Validation of Homophobia Scale (HS

    Directory of Open Access Journals (Sweden)

    Giacomo Ciocca, PsyD, PhD

    2015-09-01

    Conclusions: The Italian validation of the HS revealed the use of this self‐report test to have good psychometric properties. This study offers a new tool to assess homophobia. In this regard, the HS can be introduced into the clinical praxis and into programs for the prevention of homophobic behavior. Ciocca G, Capuano N, Tuziak B, Mollaioli D, Limoncin E, Valsecchi D, Carosa E, Gravina GL, Gianfrilli D, Lenzi A, and Jannini EA. Italian validation of Homophobia Scale (HS. Sex Med 2015;3:213–218.

  14. Pyoderma gangrenosum, acne and suppurative hidradenitis syndrome following bowel bypass surgery.

    Science.gov (United States)

    Marzano, Angelo V; Ishak, Rim S; Colombo, Antonella; Caroli, Francesco; Crosti, Carlo

    2012-01-01

    The clinical triad of pyoderma gangrenosum (PG), acne and suppurative hidradenitis (PASH) has recently been described as a new disease entity within the spectrum of autoinflammatory syndromes, which are an emerging group of inflammatory diseases distinct from autoimmune, allergic and infectious disorders. PASH syndrome is similar to PAPA (pyogenic arthritis, acne and PG), but it differs in lacking the associated arthritis and on a genetic basis. PAPA syndrome is caused by mutations in a gene involved in the regulation of innate immune responses, the PSTPIP1, while no mutations have been detected to date in patients with PASH syndrome. We report a young male patient who developed coexisting disseminated PG, typical suppurative hidradenitis and acneiform eruption on the face, after he had undergone bowel bypass surgery for obesity. The cutaneous manifestations associated with bowel bypass syndrome often mimic PG or other neutrophilic dermatoses, suggesting a pathogenesis related to neutrophil-mediated inflammation for this condition. This is the first report describing PASH syndrome after bariatric surgery, and we propose to include such neutrophilic dermatoses in the list of complications occurring after bowel bypass surgery. Extensive genetic studies may help to clarify the etiopathogenesis of PASH as well as of autoinflammatory diseases in general.

  15. Italian Validation of Homophobia Scale (HS).

    Science.gov (United States)

    Ciocca, Giacomo; Capuano, Nicolina; Tuziak, Bogdan; Mollaioli, Daniele; Limoncin, Erika; Valsecchi, Diana; Carosa, Eleonora; Gravina, Giovanni L; Gianfrilli, Daniele; Lenzi, Andrea; Jannini, Emmanuele A

    2015-09-01

    The Homophobia Scale (HS) is a valid tool to assess homophobia. This test is self-reporting, composed of 25 items, which assesses a total score and three factors linked to homophobia: behavior/negative affect, affect/behavioral aggression, and negative cognition. The aim of this study was to validate the HS in the Italian context. An Italian translation of the HS was carried out by two bilingual people, after which an English native translated the test back into the English language. A psychologist and sexologist checked the translated items from a clinical point of view. We recruited 100 subjects aged18-65 for the Italian validation of the HS. The Pearson coefficient and Cronbach's α coefficient were performed to test the test-retest reliability and internal consistency. A sociodemographic questionnaire including the main information as age, geographic distribution, partnership status, education, religious orientation, and sex orientation was administrated together with the translated version of HS. The analysis of the internal consistency showed an overall Cronbach's α coefficient of 0.92. In the four domains, the Cronbach's α coefficient was 0.90 in behavior/negative affect, 0.94 in affect/behavioral aggression, and 0.92 in negative cognition, whereas in the total score was 0.86. The test-retest reliability showed the following results: the HS total score was r = 0.93 (P homophobia. In this regard, the HS can be introduced into the clinical praxis and into programs for the prevention of homophobic behavior.

  16. HS06 Benchmark for an ARM Server

    Science.gov (United States)

    Kluth, Stefan

    2014-06-01

    We benchmarked an ARM cortex-A9 based server system with a four-core CPU running at 1.1 GHz. The system used Ubuntu 12.04 as operating system and the HEPSPEC 2006 (HS06) benchmarking suite was compiled natively with gcc-4.4 on the system. The benchmark was run for various settings of the relevant gcc compiler options. We did not find significant influence from the compiler options on the benchmark result. The final HS06 benchmark result is 10.4.

  17. HS06 Benchmark for an ARM Server

    CERN Document Server

    Kluth, Stefan

    2013-01-01

    We benchmarked an ARM cortex-A9 based server system with a four-core CPU running at 1.1 GHz. The system used Ubuntu 12.04 as operating system and the HEPSPEC 2006 (HS06) benchmarking suite was compiled natively with gcc-4.4 on the system. The benchmark was run for various settings of the relevant gcc compiler options. We did not find significant influence from the compiler options on the benchmark result. The final HS06 benchmark result is 10.4.

  18. VIRTUAL REALITY HYPNOSIS FOR PAIN CONTROL IN A PATIENT WITH GLUTEAL HIDRADENITIS:A CASE REPORT().

    Science.gov (United States)

    Soltani, Maryam; Teeley, Aubriana M; Wiechman, Shelley A; Jensen, Mark P; Sharar, Sam R; Patterson, David R

    2011-01-01

    This case report describes the use of hypnotic analgesia induced through immersive three-dimensional computer-generated virtual reality, better known as virtual reality hypnosis (VRH), in the treatment of a patient with ongoing pain associated with gluteal hidradenitis, The patient participated in the study for two consecutive days white hospitalized at a regional trauma centre. At pretreatment, she reported severe pain intensity and unpleasantness as well as high levels of anxiety and nervousness. She was then administered two sessions of virtual reality hypnotic treatment for decreased pain and anxiety. The patient's ratings of 'time spent thinking about pain', pain intensity, 'unpleasantness of pain', and anxiety decreased from before to after each daily VRH session, as well as from Day One to Day Two. The findings indicate that VRH may benefit individuals with severe, ongoing pain from a chronic condition, and that a controlled clinical trial examining its efficacy is warranted.

  19. DHS HS-STEM Final Report.

    Energy Technology Data Exchange (ETDEWEB)

    Russell, Anna Christine [Sandia National Laboratories (SNL-CA), Livermore, CA (United States)

    2014-09-01

    Throughout my HS-STEM internship, I worked on two different projects with a systems analysis group at Sandia National Laboratories in Livermore, California (SNLCA). The first, and primary, project entailed building a conceptual model of health surveillance detection of a bioterror attack. The second project was much smaller in scope and looked at cost tradeoffs between volumetric and surface decontamination after the release of anthrax in a city. Both projects helped me to understand the challenges of planning for a bioterror attack and the importance of preparedness in the public health sector.

  20. the doubly magic nucleus 270Hs

    Directory of Open Access Journals (Sweden)

    Silisteanu I.

    2016-01-01

    Full Text Available The α-decay and spontaneous fission half-lives of nuclei around 270Hs are calculated with formulas derived from an in-depth analysis of the available experimental data and of results of theoretical models. The parameters of these formulas result from the fit of half-lives with respect to the reaction energies, the height of the SF barrier and the fissionability. The calculated partial and total half-lives are compared with the experimental data and results of other approximations. Half-life predictions are made for many unknown nuclei.

  1. Occupational Accident Declaration Form (HS50)

    CERN Multimedia

    HR Department

    2007-01-01

    https://cern.ch/service-procedures/AdminMan/Forms/HS50E.doc •\tIt must be completed within 10 working days of the date on which the accident occurred (§ 29.2.1), unless the person concerned is materially unable to meet this deadline. • The completed formula must be accompanied by a medical certificate giving details of any bodily injuries resulting from the accident (Annex 1, § 5). The medical certificate must be obtained from the doctor who has been consulted for that purpose. Benefits resulting from illnesses and accidents Medical treatment will cease to be reimbursed under the occupational scheme in the event of cure (defined in § 15 as the certified end to the impairment of the patient’s state of health caused by the illness or accident), or consolidation (defined in § 14 as certification that no further improvement in the patient’s state of health can be expected from appropriate medical treatment). The right to such reimbursement shall resume only in the...

  2. Analysis of the HI-6, HS-3 and HS-6, Influence on the Liver Methabolizing Enzyme Systems

    Science.gov (United States)

    2001-09-01

    ON THE LIVER METHABOLIZING ENZIME SYSTEMS Christophor Dishovsky, Maria Kadiiska*, Petko Alov* Military Medical Academy, 1606, Sofia, Bulgaria...reactivators of ChE, liver, methabolizing enzime systems FIGURES AND TABLES Table 1. Effect of reactivators of ChE HS-3, HS-6, HI-6 on the hexobarbital

  3. Pyogenic Arthritis, Pyoderma Gangrenosum, Acne, Suppurative Hidradenitis (PA-PASH) Syndrome: An Atypical Presentation of a Rare Syndrome

    Science.gov (United States)

    Ursani, Mohammad A.; Appleyard, Joan; Whiteru, Onome

    2016-01-01

    Patient: Male, 44 Final Diagnosis: PAPASH syndrome Symptoms: Recurrent skin ulcers • diarrhea • inflammatory arthritis Medication: Prednisone • anti-tumor necrosis factor Clinical Procedure: N/A Specialty: Rheumatology Objective: Rare disease Background: Pyogenic arthritis, pyoderma gangrenosum (PG), acne, and suppurative hidradenitis (PA-PASH) syndrome has been linked to an auto-inflammatory pathway. We report a case that is an atypical presentation of a rare syndrome, which supports literature suggesting that different phenotypes of PG-related syndromes may be a variation of the same pathogenic spectrum. Interestingly, our patient displayed a positive proteinase-3 antibody (PR-3). The clinical relevance of this is unclear. In recent literature, antineutrophil cytoplasmic autoantibodies (ANCA) positivity has been reported in various inflammatory conditions other than ANCA-associated vasculitis (AAV). Case Report: A 44-year-old African American male with history of pyogenic arthritis, acne, suppurative hidradenitis, and chronic diarrhea presented for evaluation of painful ulcers located on the bilateral lower extremities, bilateral proximal interphalangeal joints, buttocks, and scrotum, and chronic diarrhea. Infectious etiologies for the ulcers were ruled out. Biopsy of an ulcer revealed PG. Colonoscopy revealed inflammation and ulceration with biopsy consistent with ulcerative colitis (UC). After treatment with prednisone, the ulcers healed within 4 weeks, and the chronic diarrhea resolved. Conclusions: Our patient displayed a variation of PA-PASH syndrome and UC. Previously reported cases of similar phenotypes of PG-related syndromes have not presented in this fashion. Furthermore, the literature does not report cases of PG-related syndromes with an elevation in PR-3 antibody. Elevation in PR-3 has been reported in various inflammatory disorders aside from AAV. The relevance of this is currently unclear. It may be possible that the milieus of these various

  4. Spin Crossover in Dinuclear N4S2 Iron(II) Thioether-Triazole Complexes: Access to [HS-HS], [HS-LS], and [LS-LS] States.

    Science.gov (United States)

    Hogue, Ross W; Feltham, Humphrey L C; Miller, Reece G; Brooker, Sally

    2016-05-01

    Access to a new family of thioether-linked PSRT ligands, 4-substituted-3,5-bis{[(2-pyridylmethyl)sulfanyl]methyl}-4H-1,2,4-triazoles (analogues of the previously studied amino-linked PMRT ligands), has been established. Four such ligands have been prepared, PSPhT, PS(i)BuT, PS(t-Bu)PhT, and PS(Me)PhT, with R = Ph, (i)Bu, (t-Bu)Ph, and (Me)Ph, respectively. Three dinuclear colorless to pale green iron(II) complexes, [Fe(II)2(PSRT)2](BF4)4·solvent, featuring N4S2 donor sets, were prepared. Single-crystal structure determinations on [Fe(II)2(PSPhT)2](BF4)4·2MeCN·H2O, [Fe(II)2(PSPhT)2](BF4)4·2(1)/2MeCN·(1)/2H2O·THF, [Fe(II)2(PS(Me)PhT)2](BF4)4·2MeCN, and [Fe(II)2(PS(i)BuT)2](BF4)4·4MeCN reveal that all four are stabilized in the [HS-HS] state to 100 K and that both possible binding modes of the bis-terdentate ligands, cis- and trans-axial, are observed. Variable-temperature magnetic susceptibility studies of air-dried crystals (solvatomorphs of the single crystal samples) reveal the first examples of spin crossover (SCO) for a dinuclear iron(II) complex with N4S2 coordination. Specifically, [Fe(II)2(PSPhT)2](BF4)4·2(1)/2H2O undergoes a multistep but complete SCO from [HS-HS] to [LS-LS], whereas [Fe(II)2(PS(Me)PhT)2](BF4)4·1(1)/2MeCN·2H2O exhibits a half-SCO from [HS-HS] to [HS-LS]. In contrast, [Fe(II)2(PS(i)BuT)2](BF4)4·MeCN·H2O remains [HS-HS] down to 50 K. The reflectance spectrum of pale green [Fe(II)2(PSPhT)2](BF4)4·(1)/2CHCl3·2(1)/2H2O (solvatomorph A) reveals a trace of LS character (572 nm band (1)A1g → (1)T1g). Evans' (1)H NMR method and UV-vis spectroscopy studies revealed that on cooling dark green acetonitrile solutions of these complexes from 313 to 233 K, all three undergo SCO centered at or near room temperature. The tendency of the complexes to go LS in solution reflects the electronic impact of R on the σ-donor strength of the PSRT ligand, whereas the opposite trend in stabilization of the LS state is seen in the solid state, where

  5. Developing competitive marketing and sales strategy for HS-Eden

    OpenAIRE

    Dragusha, Cajup

    2016-01-01

    Start-ups are faced with variety of challenges and uncertainty therefore comprehensive marketing and sales strategy must be in place to make sure that limited resources are spend wisely in order to minimize uncertainty and pave a path that would lead to successful business. HS Eden is a new-start-up venture created in Lappeenranta University of Technology with an ambition to develop AMB systems for commercial use. The aim of this study was to build a marketing and sales strategy to help HS-Ed...

  6. Molecular Analysis of HS-111 and 3`HS1 Variations in β-Thalassemia Intermedia Patients with High Levels of HbF

    Directory of Open Access Journals (Sweden)

    Mohammad Hamid

    2010-01-01

    Full Text Available Objective: To study the possible association between high levels of fetal haemoglobin(HbF in β-thalassemia intermedia patients and HS-111 and 3`HS1 sequence variations.Materials and Methods: In this study, the 3' HS-1 and HS-111 regions of 30 ß-thalassaemiaintermedia patients (ß°/ß° with high levels of HbF, 21 ß-thalassemia major patientsand 40 normal Iranian individuals were analyzed by single-strand conformation polymorphism(SSCP and polymerase chain reaction (PCR sequencing.Results: Two nucleotide variations in 3' HS111 (-21A>G and 3`HS1 (179C>T wereidentified. The most frequent sequence variation was 3' HS111 (-21A in the intermediapatients and 3`HS111 (-21G in the major thalassemia patients. In contrast to the 3`HS1marker, both 3'HS111 A and G variants showed a correlation with each studied group.Conclusion: The HS111 marker in conjunction with other parameters could be used asappropriate genetic markers to discriminate β-thalassemia intermedia patients (β°/β° withhigh levels of HbF from β-thalassemia major patients.

  7. Jónsson and HS Modules over Commutative Rings

    Directory of Open Access Journals (Sweden)

    Greg Oman

    2014-01-01

    Full Text Available Let R be a commutative ring with identity and let M be an infinite unitary R-module. (Unless indicated otherwise, all rings are commutative with identity 1≠0 and all modules are unitary. Then M is called a Jónsson module provided every proper submodule of M has smaller cardinality than M. Dually, M is said to be homomorphically smaller (HS for short if |M/N|<|M| for every nonzero submodule N of M. In this survey paper, we bring the reader up to speed on current research on these structures by presenting the principal results on Jónsson and HS modules. We conclude the paper with several open problems.

  8. High-speed Neural Network HS-K-WTA-2%高速神经网络HS-K-WTA-2的研究

    Institute of Scientific and Technical Information of China (English)

    朱红; 陈清华; 刘国岁

    2007-01-01

    该文提出了一种新的K-Winners-Take-All神经网络:High-Speed-K-Winners-Take-All-2(HS-K-WTA-2).HS-K-WTA-2以竞争学习算法为基础.HS-K-WTA-2能够从任何一个数集中识别出K个较大的数,或K个较小的数.该文给出HS-K-WTA-2算法及算法复杂度的分析结果.用专门为研究K-WTA神经网络开发的仿真程序对HS-K-WTA-2、HS-K-WTA和Winstrons进行仿真研究.结果显示:当所取的数集N较大时,HS-K-WTA-2要比Winstrons和HS-K-WTA速度更快.HS-K-WTA-2的硬件实现比Winston的硬件实现要简单,比HS-K-WTA的硬件实现复杂.

  9. High Speed Oblivious Random Access Memory (HS-ORAM)

    Science.gov (United States)

    2015-09-01

    and Automated Teller Machines ( ATM ) security while generally impractical due to performance limitations and high acquisition costs. This idea has...HIGH SPEED OBLIVIOUS RANDOM ACCESS MEMORY (HS-ORAM) PRIVATE MACHINES , INC. SEPTEMBER 2015 FINAL TECHNICAL REPORT...UNIT NUMBER I1 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Private Machines Inc. 164 20th Street #3D Brooklyn, NY 11232 8. PERFORMING

  10. Metformin - For the dermatologist

    Directory of Open Access Journals (Sweden)

    Aditya Kumar Bubna

    2016-01-01

    Full Text Available Metformin though primarily an antidiabetic drug, has found to play an important role in a number of cutaneous disorders. Because of its role in improving hyperinsulinemia, it has proven beneficial in hormonal acne, hidradenitis suppurativa (HS and acanthosis nigricans. Its antiandrogenic properties further serve as an add-on to the conventional management of hirsutism associated with polycystic ovarian syndrome. Very recently, systemic usage of metformin for psoriasis and cutaneous malignancies has shown promising results. Interestingly, metformin has also been topically used in hyperpigmentary disorders with pertinent levels of improvement and happens to be the most recent addition to the list of dermatologic indications. Though an oral hypoglycemic agent to begin with, metformin today has proven to be a boon for dermatologists.

  11. Metformin - For the dermatologist.

    Science.gov (United States)

    Bubna, Aditya Kumar

    2016-01-01

    Metformin though primarily an antidiabetic drug, has found to play an important role in a number of cutaneous disorders. Because of its role in improving hyperinsulinemia, it has proven beneficial in hormonal acne, hidradenitis suppurativa (HS) and acanthosis nigricans. Its antiandrogenic properties further serve as an add-on to the conventional management of hirsutism associated with polycystic ovarian syndrome. Very recently, systemic usage of metformin for psoriasis and cutaneous malignancies has shown promising results. Interestingly, metformin has also been topically used in hyperpigmentary disorders with pertinent levels of improvement and happens to be the most recent addition to the list of dermatologic indications. Though an oral hypoglycemic agent to begin with, metformin today has proven to be a boon for dermatologists.

  12. File list: Oth.Epd.05.AllAg.Hs_68 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Epd.05.AllAg.Hs_68 hg19 TFs and others Epidermis Hs 68 SRX396584,SRX396581,SRX3...96579,SRX396580,SRX396583,SRX396590,SRX396589 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/Oth.Epd.05.AllAg.Hs_68.bed ...

  13. File list: NoD.Epd.50.AllAg.Hs_68 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Epd.50.AllAg.Hs_68 hg19 No description Epidermis Hs 68 SRX007038,SRX007037,SRX0...07036,SRX007035 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.Epd.50.AllAg.Hs_68.bed ...

  14. File list: NoD.Epd.10.AllAg.Hs_68 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Epd.10.AllAg.Hs_68 hg19 No description Epidermis Hs 68 SRX007036,SRX007035,SRX0...07038,SRX007037 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.Epd.10.AllAg.Hs_68.bed ...

  15. File list: NoD.Epd.20.AllAg.Hs_68 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Epd.20.AllAg.Hs_68 hg19 No description Epidermis Hs 68 SRX007036,SRX007038,SRX0...07037,SRX007035 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/NoD.Epd.20.AllAg.Hs_68.bed ...

  16. Method development and analysis of free HS and HS in proteoglycans from pre- and postmenopausal women: evidence for biosynthetic pathway changes in sulfotransferase and sulfatase enzymes.

    Science.gov (United States)

    Wei, Wei; Miller, Rebecca L; Leary, Julie A

    2013-06-18

    Heparan sulfate (HS) is one of the most complex and informative biopolymers found on the cell surface or in the extracellular matrix as either free HS fragments or constituents of HS proteoglycans (HSPGs). Analysis of free HS and HSPG sugar chains in human serum at the disaccharide level has great potential for early disease diagnosis and prognosis; however, the low concentration of HS in human serum, together with the complexity of the serum matrix, limits the information on HS. In this study, we present and validate the development of a new sensitive method for in-depth compositional analysis of free HS and HSPG sugar chains. This protocol involved several steps including weak anion exchange chromatography, ultrafiltration, and solid-phase extraction for enhanced detection prior to LC-MS/MS analysis. Using this protocol, a total of 51 serum samples from 26 premenopausal and 25 postmenopausal women were analyzed. Statistically significant differences in heparin/HS disaccharide profiles were observed. The proportion of N-acetylation and N-sulfation in both free HS and HSPG sugar chains were significantly different between pre- and postmenopausal women, indicating changes in N-deacetylase/N-sulfotransferases (NDSTs), the enzymes involved in the initial step of the biosynthetic pathway. Differences in the proportion of 6-O-sulfation suggest that 6-O-sulfotransferase and/or 6-O-sulfatase enzymes may also be implicated.

  17. Reduced beta-globin gene expression in adult mice containing deletions of locus control region 5' HS-2 or 5' HS-3.

    Science.gov (United States)

    Ley, T J; Hug, B; Fiering, S; Epner, E; Bender, M A; Groudine, M

    1998-06-30

    To gain insights into the functions of individual DNA'se hypersensitive sites within the beta globin locus control region (LCR), we deleted the endogenous 5' HS-2 and HS-3 regions from the mouse germline using homologous recombination techniques. We demonstrated that the deletion of either murine 5' HS-2 or 5' HS-3 reduced the expression of the embryonic epsilon y and beta h1 globin genes minimally in yolk sac-derived erythrocytes, but that both knockouts reduced the output of the adult beta (beta-Major + beta-Minor) globin genes by approximately 30% in adult erythrocytes. When the selectable marker PGK-Neo cassette was retained within either the HS-2 or HS-3 region, a much more severe reduction in globin gene expression was observed at all developmental stages. PGK-Neo was shown to be expressed in an erythroid-specific fashion when it was retained in the HS-3 position. These results show that neither 5' HS-2 nor HS-3 is required for the activity of embryonic globin genes, nor are these sites required for correct developmental switching. However, each site is required for approximately 30% of the total LCR activity associated with adult beta-globin gene expression in adult red blood cells. Each site therefore contains some non-redundant information that contributes to adult globin gene function.

  18. Neural-Based Cuckoo Search of Employee Health and Safety (HS

    Directory of Open Access Journals (Sweden)

    Koffka Khan

    2013-01-01

    Full Text Available A study using the cuckoo search algorithm to evaluate the effects of using computer-aided workstations on employee health and safety (HS is conducted. We collected data for HS risk on employees at their workplaces, analyzed the data and proposed corrective measures applying our methodology. It includes a checklist with nine HS dimensions: work organization, displays, input devices, furniture, work space, environment, software, health hazards and satisfaction. By the checklist, data on HS risk factors are collected. For the calculation of an HS risk index a neural-swarm cuckoo search (NSCS algorithm has been employed. Based on the HS risk index, IHS four groups of HS risk severity are determined: low, moderate, high and extreme HS risk. By this index HS problems are allocated and corrective measures can be applied. This approach is illustrated and validated by a case study. An important advantage of the approach is its easy use and HS index methodology speedily pointing out individual employee specific HS risk.

  19. Nonexistence of Weyl-Heisenberg Frame on Sobolev Space Hs(Rd)%Sobolev 空间Hs(Rd)上的Weyl-Heisenberg框架的不存在性

    Institute of Scientific and Technical Information of China (English)

    李锐; 郝祥晖; 李登峰

    2002-01-01

    研究在窗函数的Fourier变换具有紧支集条件下,Sobolev空间Hs(Rd)上不存在Weyl-Heisenberg框架.这个结果表明:对Sobolev空间Hs(Rd)上的Weyl-Heisenberg框架的研究意义不大.

  20. 血清NT-proBNP、hs-TnT及hs-CRP水平与急性心肌梗死的相关性研究%Correlation between serum NT-proBNP, hs-TnT and hs-CRP levels and acute myocardial infarction

    Institute of Scientific and Technical Information of China (English)

    符明昌; 周莲; 羊文芳; 苏显都

    2016-01-01

    目的:探讨血清脑钠肽前体(NT-proBNP)、高敏肌钙蛋白T (hs-TnT)、超敏C反应蛋白(hs-CRP)与急性心肌梗死(AMI)的相关性,并分析联合检测对AMI患者的诊断价值。方法选取2013年1月至2015年6月海南省第三人民医院112例AMI患者和80例体检者(对照组),分别检测AMI患者3 h、12 h、1 d、3 d、7 d血清NT-proBNP、hs-TnT及hs-CRP水平,并与对照组进行比较。绘制受试工作特征曲线(ROC)评价NT-proBNP、hs-TnT及hs-CRP在AMI中的诊断价值,并应用Logistic回归模型分析AMI的独立危险因素。结果 AMI组血清NT-proBNP、hs-TnT及hs-CRP水平明显高于对照组,差异均有统计学意义(P<0.05)。血清NT-proBNP、Hs-TnT及Hs-CRP在AMI患者中出现峰值时间分别为1 d、12 h、3 d。应用Logistic回归模型对变量进行单因素及多因素分析,发现NT-proBNP、hs-TnT及hs-CRP是发生AMI的独立危险因素,其OR值及95%CI分别为1.012(0.874~1.136)、1.350(1.026~1.724)、1.168(1.014~1.337)。ROC曲线显示,NT-proBNP、hs-TnT、hs-CRP和三项联合检测的AUC及95%CI分别为:0.860(0.784~0.975)、0.834(0.762~0.905)、0.748(0.668~0.817)、0.885(0.794~0.971)。Spearman相关分析显示,NT-proBNP与hs-TnT呈明显正相关(r=0.679,P<0.05),NT-proBNP与hs-CRP呈明显正相关(r=0.602,P<0.05),hs-TnT与hs-CRP呈明显正相关(r=0.568,P<0.05)。结论 NT-proBNP、hs-TnT及hs-CRP是AMI发生的独立预测因子,联合检测可提高AMI诊断的准确性,有助于早期治疗并改善预后。%Objective To investigate the diagnostic value of serum N-terminal pro-brain natriuretic peptide (NT-proBNP), high-sensitivity troponin T (hs-TnT) and hypersensitive C-reactive protein (hs-CRP) in acute myocardial infarction (AMI), and to analyze their correlation. Methods A total of 112 patients of AMI (AMI group) and 80 sub-jects of physical examination (control group) in Third People's Hospital of Hainan Province were selected from January

  1. Assessment of cytokine levels and hs-CRP in bipolar I disorder before and after treatment.

    Science.gov (United States)

    Uyanik, Vesile; Tuglu, Cengiz; Gorgulu, Yasemin; Kunduracilar, Hakan; Uyanik, Mehmet Sevki

    2015-08-30

    We aimed to assess the relationship between cytokine levels and the severity of the manic period in medication free patients. 30 Medication free patients and 28 healthy subjects (HS) were recruited. Plasma levels of pro-inflammatory, anti-inflammatory, inflammatory cytokines, and hs-CRP levels were investigated upon hospital admission, after six weeks follow up in bipolar disease manic episode and the results were compared to HS. The severity of the manic episodes was assessed according to the Young mania rating scale. TNF-α, INF-γ, IL-6 and hs-CRP levels were significantly higher in patients with manic episode of bipolar I disorder before treatment than HS. After treatment the levels of TNF-α, INF-γ, IL-6 and hs-CRP were observed to be significantly decreased. There was no difference between the levels of anti-inflammatory cytokines in patients before or after treatment of bipolar disorder and HS. hs-CRP was observed to be the only parameter correlated with clinical response. The most significant outcome of this study is the correlation between clinical outcome and hs-CRP levels in treatment naive manic episode bipolar type I patients. hs-CRP is the most consistent indicator according to pro-inflammatory, inflammatory and anti-inflammatory cytokines, in predicting treatment outcomes.

  2. N=2 gauge theories on the hemisphere $HS^4$

    CERN Document Server

    Gava, Edi; Muteeb, Nouman; Giraldo-Rivera, V I

    2016-01-01

    Using localization techniques, we compute the path integral of $N=2$ SUSY gauge theory coupled to matter on the hemisphere $HS^4$, with either Dirichlet or Neumann supersymmetric boundary conditions. The resulting quantities are wave-functions of the theory depending on the boundary data. The one-loop determinant are computed using $SO(4)$ harmonics basis. We solve kernel and co-kernel equations for the relevant differential operators arising from gauge and matter localizing actions. The second method utilizes full $SO(5)$ harmonics to reduce the computation to evaluating $Q_{SUSY}^2$ eigenvalues and its multiplicities. In the Dirichlet case, we show how to glue two wave-functions to get back the partition function of round $S^4$. We will also describe how to obtain the same results using $SO(5)$ harmonics basis.

  3. 3-O-sulfated oligosaccharide structures are recognized by anti-heparan sulfate antibody HS4C3.

    NARCIS (Netherlands)

    Dam, G.B. ten; Kurup, S.; Westerlo, E.M. van de; Versteeg, E.M.M.; Lindahl, U.; Spillmann, D.; Kuppevelt, A.H.M.S.M. van

    2006-01-01

    Antibodies against heparan sulfate (HS) are useful tools to study the structural diversity of HS. They demonstrate the large sequential variation within HS and show the distribution of HS oligosaccharide sequences within their natural environment. We analyzed the distribution and the structural char

  4. Increased Hs-CRP/adiponectin ratio is associated with increase carotid intima-media thickness

    OpenAIRE

    Liao, Huocheng; Li, Zhiming; Zheng, Dongdan; Liu, Jianping; Liu, Yan; Xiao, Chun; Wang, Hongguang

    2014-01-01

    Background High sensitivity C-reactive protein (Hs-CRP) and adiponectin (APN) are two critical cytokines and exert inverse effects on atherosclerosis initiation and progression. The purpose of our study was to investigate the value of Hs-CRP and ANP ratio (Hs-CRP/APN ratio) on evaluating atherosclerosis progression. Method One hundred sixty consecutive participants underwent carotid intima-media thickness (CIMT) measured by ultrasound were enrolled and drawn fasting blood samples for plasma l...

  5. Kadar hs-CRP pada pasien DM tipe 2 Dengan dan Tanpa Hipertensi

    OpenAIRE

    Ramadhani, Efi

    2016-01-01

    Introduction: More than 50% of patients with type-2 diabetes have hypertension. Hypertension contributes the development of cardiovascular disease. hs-CRP is an inflammation marker which is associated with cardiovascular risk. The aim of this study is to measure hs-CRP in type-2 diabetes with and without hypertension and to assess hs-CRP levels based on cardiovascular risk group. Methods: This study is an analytical observational cross-sectional study. Performed at the Department of Clini...

  6. The possible existence of Hs in nature from a geochemical point of view

    CERN Document Server

    Ivanov, A V

    2006-01-01

    A hypothesis of the existence of a long-lived isotope 271Hs in natural molybdenites and osmirides is considered from a geochemical point of view. It is shown that the presence of Hs in these minerals can be explained only by making an additional ad hoc assumption on the existence of an isobaric pair of 271Bh-271Hs. This assumption could be tested by mass-spectrometric measurements of U, Pb, Kr, Xe, and Zr isotopic shifts.

  7. Hcy与hs-CRP测定在CHD诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    李林

    2009-01-01

    @@ 越来越多的研究表明,同型半胱氨酸(Hcy)、超敏C-反应蛋白(hs-CRP)是动脉粥样硬化独立的危险因素[1~2].本文通过检测冠心病(CHD)患者与对照组血中的Hcy、hs-CRP水平,探讨CHD的严重程度与Hcy、hs-CRP的相关性.

  8. Component C of the methylcoenzyme M methylreductase system contains bound 7-mercaptoheptanoylthreonine phosphate (HS-HTP).

    Science.gov (United States)

    Noll, K M; Wolfe, R S

    1986-09-30

    The structure of component B of the methylcoenzyme M methylreductase system of Methanobacterium thermoautotrophicum was recently found to be 7-mercaptoheptanoylthreonine phosphate (HS-HTP). The work described here demonstrates that this compound is found in two forms: enzyme-free and enzyme-bound. HS-HTP was found to be bound to component C of the methylcoenzyme M methylreductase system. The cofactor extracted from the protein by heat denaturation was found to comigrate with the mixed disulfide of HS-HTP and 2-mercaptoethanol by high-performance liquid chromatography, suggesting HS-HTP is not modified in the bound state.

  9. In vivo redundant function of the 3' IgH regulatory element HS3b in the mouse.

    Science.gov (United States)

    Bébin, Anne-Gaëlle; Carrion, Claire; Marquet, Marie; Cogné, Nadine; Lecardeur, Sandrine; Cogné, Michel; Pinaud, Eric

    2010-04-01

    In the mouse, the regulatory region located at the 3' end of the IgH locus includes four transcriptional enhancers: HS3a, HS1-2, HS3b, and HS4; the first three lie in a quasi-palindromic structure. Although the upstream elements HS3a and HS1-2 proved dispensable for Ig expression and class switch recombination (CSR), the joint deletion of HS3b and HS4 led to a consistent decrease in IgH expression in resting B cells and to a major CSR defect. Within this pair of distal enhancers, it was questionable whether HS3b and HS4 could be considered individually as elements critical for IgH expression and/or CSR. Studies in HS4-deficient mice recently revealed the role of HS4 as restricted to Igmicro-chain expression from the pre-B to the mature B cell stage and left HS3b as the last candidate for CSR regulation. Our present study finally invalidates the hypothesis that CSR could mostly rely on HS3b itself. B cells from HS3b-deficient animals undergo normal proliferation, germline transcription, and CSR upon in vitro stimulation with LPS; in vivo Ag-specific responses are not affected. In conclusion, our study highlights a major effect of the global ambiance of the IgH locus; enhancers demonstrated as being strongly synergistic in transgenes turn out to be redundant in their endogenous context.

  10. Sistem Informasi Penjualan Pemasangan Kacafilm “HS Auto Film"

    Directory of Open Access Journals (Sweden)

    Noer Tjahja Moekthi Pratjitana

    2013-07-01

    Full Text Available The growing market share in many business areas especially in service area is causing the use of conventional accounting system being ineffective. One of the companies which still using the conventional one is a window film set-up service company called HS Auto Film. The process of processing sales data is less accurate and slower in the contrary of the developing sales data.  Computer-based window film set-up Sales Information System is built in order to make the processing data more accurate and faster.  The information system is built using Visual Basic programming language along with .NET framework technology and SQL Server database that embedded in the application itself. The making and development process of the Sales Information System is using waterfall method.  Waterfall method consists of user requirement, analysis, design, implementation, and testing. Entity Relationship Diagram, Data Flow Diagram and Flowchart are also used in the Information System design. The output of this application design is a desktop application that can help user to control the set-up of window film, manage payment bill, and manage document in accounting in faster and more accurate way. The information system can be developed later using web-based system so that the development and maintenance can be done easier.

  11. Complete genomic sequence of campylobacter jejuni subsp. jejuni HS:19 penner reference strain

    Science.gov (United States)

    Campylobacter jejuni subsp. jejuni (Cjj) infections are a leading cause of foodborne gastroenteritis and the most prevalent antecedent to Guillain-Barré syndrome (GBS). Capsular type Penner HS:19 is among several capsule types shown to be markers for GBS. This study describes the genome of Cjj HS:19...

  12. Genomic Sequence of Campylobacter jejuni subsp. jejuni HS:19 Penner Serotype Reference Strain RM3420

    Science.gov (United States)

    Huynh, Steven; Heikema, Astrid P.

    2017-01-01

    ABSTRACT Campylobacter jejuni subsp. jejuni infections are a leading cause of foodborne gastroenteritis and the most prevalent antecedent to Guillain-Barré syndrome (GBS). Penner serotype HS:19 is among several capsular types shown to be markers for GBS. This study describes the genome of C. jejuni subsp. jejuni HS:19 Penner reference strain RM3420. PMID:28232429

  13. Vegetable and Fruit Intakes Are Associated with hs-CRP Levels in Pre-Pubertal Girls.

    Science.gov (United States)

    Navarro, Pilar; de Dios, Olaya; Jois, Asha; Gavela-Pérez, Teresa; Gorgojo, Lydia; Martín-Moreno, José M; Soriano-Guillen, Leandro; Garcés, Carmen

    2017-03-02

    The influence of diet on inflammation in children remains unclear. We aimed to analyze the influence of diet on high-sensitivity C-reactive protein (hs-CRP) levels in a pre-pubertal population free of other influences that may affect hs-CRP levels. We determined hs-CRP levels in 571 six- to eight-year-old children using an hs-CRP ELISA kit. Information on food and nutrient intake was obtained through a food-frequency questionnaire. Overall dietary quality was assessed using the Healthy Eating Index (HEI). We found that girls in the highest tertile of hs-CRP levels had a higher intake of saturated fatty acid, and lower intakes of fiber and vitamin E and a lower HEI score when compared to those in tertiles 1 and 2. We also observed a significant decrease in fruit and vegetable intakes by hs-CRP tertile. Factor analysis showed that a dietary pattern that was loaded most strongly with vegetable, fruit, fiber and vitamin A and E intakes correlated negatively (-0.132, p hs-CRP. No such association was found in boys. In conclusion, our data show that girls with a poorer quality diet show higher hs-CRP levels already at a pre-pubertal age.

  14. HS-4, a highly potent inhibitor of cell proliferation of human cancer cell

    Institute of Scientific and Technical Information of China (English)

    Gui-Lan Xing; Shu-Hong Tian; Xue-Li Xie; Jian Fu

    2015-01-01

    Objective:To investigate the antitumor activity of the compound HS-4 and the action mechanism.Methods:MTT method was used to testin vitroantitumor activity of the compound HS-4. Orthotopic xenotransplantation tumor model of liver cancer was established in nude mice, and,in vivoantitumor activity of compound HS-4 was tested with a small animal in-vivo imaging system. Sequencing of small RNA library and RNA library was performed in HS-4 treated tumor cell group and control group to investigate the anti-cancer mechanism of HS-4 at level of functional genomics, using high-throughput sequencing technology. Results:HS-4 was found to have relatively highin-vitro antitumor activity against liver cancer cells, gastric cancer cells, renal cancer cells, lung cancer cells, breast cancer cells and colon cancer cells. The IC50 values against SMMC-7721 and Bel-7402 of liver cancer cells were 0.14 and 0.13 nmol/L respectively, while the IC50 values against MGC-803 and SGC-7901 of gastric cancer cells were 0.19 and 0.21 nmol/L, respectively. It was demonstrated that HS- 4 possessed a better therapeutic effect in liver cancer.Conclusions: A new reliable orthotopic xenotransplantation tumor model of liver cancer in nude mice is established. The new compounds HS-4 was found to possess relatively highin vivo andin vitroantitumor activity against liver cancer cells.

  15. Hitachi的经济型投影机产品CP-HS900

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    CP-HS900是原Hitachi经济型产品CP-HS800的升级机型,采用3片0.55英寸液晶板和160W UHB灯泡。CP-HS900除配有CP-HS800的所有接口外还设计了RS-232C控制接口和USB鼠标控制接口,机器重量3.0kg。CP-HS900具有28dB降噪模式,演示过程中,听众不受噪音打扰。另

  16. Retinol-binding protein-4 and hs-CRP levels in patients with migraine.

    Science.gov (United States)

    Tanik, Nermin; Celikbilek, Asuman; Metin, Aslı; Gocmen, Ayse Yesim; Inan, Levent Ertugrul

    2015-10-01

    Retinol-binding protein-4 (RBP4) and high-sensitivity C-reactive protein (hs-CRP) levels are associated with inflammation in patients with migraine. The release of proinflammatory cytokines during migraine results in recurrent sterile neurogenic inflammation. This study aimed to determine the correlation between RBP4 and hs-CRP levels, and migraine, which is considered an inflammatory disease. The study included 48 migraine patients and 40 age- and gender-matched controls. Migraine was diagnosed according to International Classification of Headache Disorders-II. The serum RBP4 level was measured using a commercial ELISA kit and hs-CRP was measured using an enzyme immunoassay test kit. The serum RBP4 level was significantly lower in the migraine patients than in the controls (P hs-CRP level was significantly higher in the migraine patients (P hs-CRP levels did not differ between the migraine patients with and without aura (P > 0.05). Migraine headache severity, frequency and duration were not correlated with serum RBP or hs-CRP levels (P > 0.05). The observed high hs-CRP level and low RBP4 level in migraine patients suggest that vitamin A might play a major role in the pathogenesis of migraine. It is known that inflammation is a key factor in many diseases. Additional research might result in a better understanding of the anti-inflammatory effects of vitamin A.

  17. Changing the conversation--the occupational health nurse's role in integrated HS3.

    Science.gov (United States)

    Weiss, Marjorie D

    2009-07-01

    Occupational health nurses have the skills and knowledge to provide a holistic perspective in advancing their company's triple bottom line, healthy people, healthy planet, and healthy profits. The HS3 model provides a road map for integrating health, safety, sustainability, and stewardship, all of which directly impact every company's triple bottom line. Occupational health nurses can use the HS3 model to promote healthy lifestyles, reduce risk and injuries, protect the natural environment, and improve resource alignment. Occupational health nurses have a unique opportunity to demonstrate the value they bring to their employers using synergistic HS3 planning that cost-effectively links work injury management, health promotion, environmental protection, safety training and surveillance, and regulatory compliance. Implementing the HS3 model requires occupational health nurses to be innovators who can change the conversation.

  18. Osteoprotegerin improves risk detection by traditional cardiovascular risk factors and hsCRP

    DEFF Research Database (Denmark)

    Mogelvang, Rasmus; Haahr-Pedersen, Sune; Bjerre, Mette;

    2013-01-01

    To evaluate the association of plasma osteoprotegerin (OPG) to hospitalisation for ischaemic heart disease (IHD), ischaemic stroke and all-cause mortality, and the effect of combining plasma OPG and high-sensitivity C-reactive protein (hsCRP).......To evaluate the association of plasma osteoprotegerin (OPG) to hospitalisation for ischaemic heart disease (IHD), ischaemic stroke and all-cause mortality, and the effect of combining plasma OPG and high-sensitivity C-reactive protein (hsCRP)....

  19. HS2ST MEDIATED KIDNEY MESENCHYME INDUCTION REGULATES EARLY URETERIC BUD BRANCHING

    OpenAIRE

    Mita M. Shah; Sakurai, Hiroyuki; Sweeney, Derina E.; Gallegos, Thomas F.; Bush, Kevin T.; Esko, Jeffrey D.; Nigam, Sanjay K

    2010-01-01

    Heparan sulfate proteoglycans (HSPGs) are central modulators of developmental processes likely through their interaction with growth factors, such as GDNF, members of the FGF and TGFβ superfamilies, EGF receptor ligands and HGF. Absence of the biosynthetic enzyme, heparan sulfate 2-O-sulfotransferase (Hs2st) leads to kidney agenesis. Using a novel combination of in vivo and in vitro approaches, we have reanalyzed the defect in morphogenesis of the Hs2st−/− kidney. We observed that, while the ...

  20. Prototypic chromatin insulator cHS4 protects retroviral transgene from silencing in Schistosoma mansoni.

    Science.gov (United States)

    Suttiprapa, Sutas; Rinaldi, Gabriel; Brindley, Paul J

    2012-06-01

    Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin insulator, on transgene expression was examined. Plasmid pLNHX encoding the MLV 5'- and 3'-Long Terminal Repeats flanking the neomycin phosphotransferase gene (neo) was modified to include, within the U3 region of the 3'-LTR, active components of cHS4 insulator, the 250 bp core fused to the 400 bp 3'-region. Cultured larvae of Schistosoma mansoni were transduced with virions from producer cells transfected with control or cHS4-bearing plasmids. Schistosomules transduced with cHS4 virions expressed 2-20 times higher levels of neo than controls, while carrying comparable numbers of integrated proviral transgenes. The findings not only demonstrated that cHS4 was active in schistosomes but also they represent the first report of activity of cHS4 in any Lophotrochozoan species, which has significant implications for evolutionary conservation of heterochromatin regulation. The findings advance prospects for transgenesis in functional genomics of the schistosome genome to discover intervention targets because they provide the means to enhance and extend transgene activity including for vector based RNA interference.

  1. Evaluation of Usefulness of hs-CRP and Ferritin Assays in Patients with Nasal Polyps

    Directory of Open Access Journals (Sweden)

    Robert Partyka

    2014-01-01

    Full Text Available Background. Chronic nature of the nasal polyps, tendency to recurrence, and lack of satisfying treatment need the diagnostic’s parameters which show early inflammatory state as ferritin and hs-CRP. The Aim of Study. Assessment of hs-CRP and ferritin blood levels in nasal polyps patients in evaluation of treatment efficacy. Methods. All 38 patients were divided into 2 groups. Group I included 19 patients with anti-inflammatory therapy 2 weeks after surgery. Group II included 19 patients without anti-inflammatory therapy 2 weeks after surgery. The levels of hs-CRP and ferritin have been assessed before and 2 and 6 weeks after surgical treatment. Results. Research showed statistically significant difference of ferritin’s concentration between examined groups 6 weeks after surgery (P<0.05 and statistically significant difference of hs-CRP concentration 2 and 6 weeks after surgery (P<0.05. Conclusion. (1 The analysis of serum ferritin and hs-CRP concentrations can be useful in early postoperative detection of inflammatory state in patients with nasal polyps and for the effectiveness of therapy. (2 Lack of correlation between mean ferritin and hs-CRP serum levels, at each diagnostic and monitoring stage, shows that they are independent and cannot be determined interchangeably.

  2. A CORRELATION STUDY BETWEEN CONVENTIONAL LIPID PARAMETERS AND HS - CRP IN CORONARY HEART DISEASE

    Directory of Open Access Journals (Sweden)

    Rangaswamy

    2015-05-01

    Full Text Available BACKGROUND: Coronary heart disease (CHD is the most common form of heart disease. Changes in lifestyle, decreased physical activity, dietary modifications etc., in indian population is considered to be the major risk factor. By 2020 it is estimated that it will be th e major cause of death in the world. High sensitivity CRP (hs - CRP test measures very small amounts of CRP in the blood and is helpful even in healthy individuals to assess their potential risk for heart ailments. The hs - CRP levels are directly proportiona l to extent source of CHD which indicates that the hs - CRP has positive correlation with the disease burden. Hence the present study was undertaken. MATERIAL AND METHODS: The study was done by taking two groups controls and cases between the ages 25 - 60 year s. Fasting blood samples were collected and analysed for estimation of triglycerides, cholesterol, LDL - cholesterol, HDL - cholesterol and hs - crp levels. RESULT: Serum levels of cholesterol, TG, LDL - C, and hs - CRP (p - value 0.0001 were significantly increased and HDL - C (p - value 0.0002 was significantly decreased in cases as compared to healthy controls. CONCLUSION: It is seen that hs - CRP level is significantly elevated in cases (MI patients than the normal controls. So the present study shows a significant a nd positive correlation with conventional lipid profile parameters.

  3. Epidermal psoriasiform hyperplasia, an unrecognized sign of folliculitis decalvans: A histological study of 26 patients.

    Science.gov (United States)

    Matard, Bruno; Cavelier-Balloy, Benedicte; Reygagne, Pascal

    2017-04-01

    Follicular hyperkeratosis along with hyperplasia of the follicular and interfollicular epithelia are major histopathological characteristics of hidradenitis suppurativa (HS). The presence of an occasional thickening of lesional skin in some folliculitis decalvans (FD) patients and histological similarities between FD and HS led us to look for epidermal hyperplasia and follicular hyperkeratosis in FD patients. We performed a retrospective histological analysis of 26 patients with FD. We sought to find out whether the presence of hyperplasia of the interfollicular epidermis and of the follicular epithelia could be verified in FD, with reference to the work of von Laffert et al. concerning HS. The main quantitative and qualitative data were: follicular hyperkeratosis (77%), hyperplasia of the interfollicular epidermis (92%) with a psoriasiform aspect (88%), atrophy of the follicular epithelia (85%), plasma cells in infiltrate (92%) in large quantities (42%), follicular microcysts (60%), atrophy of the sebaceous glands (85%) and polytrichia (54%). Epidermal hyperplasia, sometimes psoriasiform and follicular microcysts, are significant histological signs of FD, which have been ignored until now although they seem very common. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  4. Retrovirus silencer blocking by the cHS4 insulator is CTCF independent

    Science.gov (United States)

    Yao, Shuyuan; Osborne, Cameron S.; Bharadwaj, Rikki R.; Pasceri, Peter; Sukonnik, Tanya; Pannell, Dylan; Recillas-Targa, Félix; West, Adam G.; Ellis, James

    2003-01-01

    Silencing of retrovirus vectors poses a significant obstacle to genetic manipulation of stem cells and their use in gene therapy. We describe a mammalian silencer blocking assay using insulator elements positioned between retrovirus silencer elements and an LCRβ-globin reporter transgene. In transgenic mice, we show that retrovirus silencers are blocked by the cHS4 insulator. Silencer blocking is independent of the CTCF binding site and is most effective when flanking the internal reporter transgene. These data distinguish silencer blocking activity by cHS4 from its enhancer blocking activity. Retrovirus vectors can be created at high titer with one but not two internal dimer cHS4 cores. cHS4 in the LTRs has no effect on expression in transduced F9 cells, suggesting that position effect blocking is not sufficient to escape silencing. The Drosophila insulators gypsy and Scs fail to block silencing in transgenic mice, but gypsy stimulates vector expression 2-fold when located in the LTRs of an infectious retrovirus. The silencer blocking assay complements existing insulator assays in mammalian cells, provides new insight into mechanisms of insulation and is a valuable tool to identify additional silencer blocking insulators that cooperate with cHS4 to improve stem cell retrovirus vector design. PMID:12954767

  5. Changes of levels of hs-CRP, Hsp70, and MPO in patients with preeclampsia

    Institute of Scientific and Technical Information of China (English)

    Xia Liu

    2016-01-01

    Objective:To observe the changes of the levels of hs-CRP, Hsp70, and MPO in patients with preeclampsia (PE) and their clinical significance. Methods:A total of 60 pregnant women with mild and severe PE who were admitted in our hospital from January, 2013 to March, 2014 were included in the study and divided into the mild group and the severe group with 30 cases in each group. The levels of serum hs-CRP, Hsp70, and MPO were detected, and their relations with PE were analyzed. The lipid metabolism related indicators were meanwhile detected. A total of 30 healthy later pregnant women at the same stage were served as the control group for comparative analysis. Results:The levels of serum hs-CRP, Hsp70, and MPO in the mild and severe PE group were significantly higher than those in the control group (P0.05). Conclusions: The abnormal lipid metabolism in different degrees exists in the pregnant women with PE, and hs-CRP, Hsp70, and MPO are together involved in the pathogenesis of PE. Detection of the levels of hs-CRP, Hsp70, and MPO in the pregnant women with PE can accurately reflect the condition of PE.

  6. Poly(lactic-co-glycolic) Acid/Solutol HS15-Based Nanoparticles for Docetaxel Delivery.

    Science.gov (United States)

    Cho, Hyun-Jong; Park, Ju-Hwan; Kim, Dae-Duk; Yoon, In-Soo

    2016-02-01

    Docetaxel (DCT) is one of anti-mitotic chemotherapeutic agents and has been used for the treatment of gastric cancer as well as head and neck cancer, breast cancer and prostate cancer. Poly(lactic- co-glycolic) acid (PLGA) is one of representative biocompatible and biodegradable polymers, and polyoxyl 15 hydroxystearate (Solutol HS15) is a nonionic solubilizer and emulsifying agent. In this investigation, PLGA/Solutol HS15-based nanoparticles (NPs) for DCT delivery were fabricated by a modified emulsification-solvent evaporation method. PLGA/Solutol HS15/DCT NPs with about 169 nm of mean diameter, narrow size distribution, negative zeta potential, and spherical morphology were prepared. The results of solid-state studies revealed the successful dispersion of DCT in PLGA matrix and its amorphization during the preparation process of NPs. According to the result of in vitro release test, emulsifying property of Solutol HS15 seemed to contribute to the enhanced drug release from NPs at physiological pH. All these findings imply that developed PLGA/Solutol HS15-based NP can be a promising local anticancer drug delivery system for cancer therapy.

  7. Follicular occlusion triad

    Directory of Open Access Journals (Sweden)

    Mittal R

    1993-01-01

    Full Text Available One 40-year female was diagnosed as a case of follicular occlusion triad (FOT as she had hidradenitis suppurativa, acne conglobata and perifolliculitis capitis abscedens et suffodiens since 7 years. Partial improvement occurred within 20 days of treatment with co-trimoxazole and haematinics but relapse occurred after 5 days of stopping antibiotics.

  8. Stabilizing Superacid Anions: The New [H(S4 O13 )2 ](3-) Anion in the Crystal Structure of Li3 [H(S4 O13 )2 ].

    Science.gov (United States)

    Schindler, Lisa Verena; Becker, Anna; Wickleder, Mathias Siegfried

    2016-12-05

    The unique hydrogenium-bis-tetrasulfate anion [H(S4 O13 )2 ](3-) in the crystal structure of Li3 [H(S4 O13 )2 ] (monoclinic, P21 /n (No. 14), Z=2, a=552.46(4) pm, b=939.70(6) pm, c=1876.6(1) pm, β=97.492(3)°, V=965.9(1)⋅10(6)  pm(3) ) is the longest protonated polysulfate chain ever observed. Very strong symmetrical hydrogen bonds are a bold feature of the crystal structure. The protonation of a very weak base such as [S4 O13 ](2-) and accordingly the stabilization of the first base of the superacid H2 S4 O13 is a significant success towards the still elusive polysulfuric acids.

  9. DEVELOPMENT OF THE HS99 AIR TRANSPORT TYPE A FISSILE PACKAGE

    Energy Technology Data Exchange (ETDEWEB)

    Blanton, P.; Eberl, K.

    2012-07-10

    An air-transport Type A Fissile radioactive shipping package for the transport of special form uranium sources has been developed by the Savannah River National Laboratory (SRNL) for the Department of Homeland Security. The Package model number is HS99 for Homeland Security Model 99. This paper presents the major design features of the HS99 and highlights engineered materials necessary for meeting the design requirements for this light-weight Type AF packaging. A discussion is provided demonstrating how the HS99 complies with the regulatory safety requirements of the Nuclear Regulatory Commission. The paper summarizes the results of structural testing to specified in 10 CFR 71 for Normal Conditions of Transport and Hypothetical Accident Conditions events. Planned and proposed future missions for this packaging are also addressed.

  10. ICARE-HS: atmospheric correction of airborne hyperspectral urban images using 3D information

    Science.gov (United States)

    Ceamanos, Xavier; Briottet, Xavier; Roussel, Guillaume; Gilardy, Hugo

    2016-10-01

    The algorithm ICARE-HS (Inversion Code for urban Areas Reflectance Extraction using HyperSpectral imagery) is presented in this paper. ICARE-HS processes airborne hyperspectral images for atmospheric compensation taking into account the strong relief of urban areas. A digital surface model is used to provide the 3D information, which is key to simulating relief-related effects such as shadow casting, multiple reflections between objects and variable illumination depending on local solid angle of view of the sky. Some of these effects are modeled using ray tracing techniques. ICARE-HS is applied to airborne hyperspectral data of the city center of Toulouse, which are also processed by a standard atmospheric correction method for comparison.

  11. Ig synthesis and class switching do not require the presence of the hs4 enhancer in the 3' IgH regulatory region.

    Science.gov (United States)

    Vincent-Fabert, Christelle; Truffinet, Véronique; Fiancette, Remi; Cogné, Nadine; Cogné, Michel; Denizot, Yves

    2009-06-01

    Several studies have reported that regulatory elements located 3' of the IgH locus (namely hs3a, hs1,2, hs3b, and hs4) might play a role during class switch recombination (CSR) and Ig synthesis. While individual deletion of hs3a or hs1,2 had no effect, pairwise deletion of hs3b (an inverted copy of hs3a) and hs4 markedly affected CSR and Ig expression. Among these two elements, hs4 was tentatively presented with the master role due to its unique status within the 3' regulatory region: distal position outside repeated regions, early activation in pre-B cells, strong activity throughout B cell ontogeny. To clarify its role, we generated mice with a clean deletion of the hs4 after replacement with a floxed neo(R) cassette. Surprisingly, and as for previous deletion of hs3a or hs1,2, deletion of hs4 did not affect either in vivo CSR or the secretion level of any Ig isotype. In vitro CSR and Ig secretion in response to LPS and cytokines was not affected either. The only noticeable effects of the hs4 deletion were a decrease in the number of B splenocytes and a decreased membrane IgM expression. In conclusion, while dispensable for CSR and Ig transcription in plasma cells, hs4 mostly appears to contribute to Ig transcription in resting B lymphocytes.

  12. Waist Circumference was Positively Correlated with Chemerin, Retinol-Binding Protein 4 and hsCRP

    Directory of Open Access Journals (Sweden)

    Lucia Herminawati

    2012-04-01

    Full Text Available BACKGROUND: Central obesity is associated with various chronic metabolic disorders characterized by abnormal cytokine production, increased acute phase reactants, and activation of inflammatory signaling pathways. This study was aimed to investigate the association of waist circumference, chemerin, and retinol binding protein (RBP-4 with inflammation in men with central obesity. METHODS: The research was conducted with a crosssectional design involving 68 centrally obese male subjects aged 30 to 60 years old, with waist circumference (WC >90 cm. All subjects fulfilled the inclusion and exclusion criteria. Anthropometric parameters, fasting glucose, creatinine, SGOT, SGPT, and hsCRP were measured. Serum concentrations of chemerin and RBP4 were measured by ELISA. RESULTS: The trend lines showed that chemerin, RBP4 and hsCRP increased with WC. Pearson correlation test showed a positively significant correlation between WC and hsCRP (r=0.242, p<0.05; and also between chemerin and hsCRP (r=0.244, p<0.05 and RBP4 (r=0.321, p<0.01. Subjects were stratified into four groups based on their chemerin and RBP4 levels (high chemerin/high RBP4, high chemerin/low RBP4, low chemerin/high RBP4, or low chemerin/low RBP4. Subjects who were in the high chemerin/low RBP4 group were more likely to have high level of inflammation (47.6%, but subjects with high chemerin/high RBP4 showed low level of inflammation (42.9% as compared with the other three groups. CONCLUSIONS: We concluded that increased WC was correlated with elevated levels of chemerin, RBP4 and hsCRP. High chemerin was correlated with increased level of RBP4 as well as with high level of inflammation. KEYWORDS: waist circumference, chemerin, RBP4, hsCRP, inflammation.

  13. Homologous elements hs3a and hs3b in the 3' regulatory region of the murine immunoglobulin heavy chain (Igh) locus are both dispensable for class-switch recombination.

    Science.gov (United States)

    Yan, Yi; Pieretti, Joyce; Ju, Zhongliang; Wei, Shiniu; Christin, John R; Bah, Fatmata; Birshtein, Barbara K; Eckhardt, Laurel A

    2011-08-01

    Immunoglobulin heavy chain (IgH) genes are formed, tested, and modified to yield diverse, specific, and high affinity antibody responses to antigen. The processes involved must be regulated, however, to avoid unintended damage to chromosomes. The 3' regulatory region of the Igh locus plays a major role in regulating class-switch recombination (CSR), the process by which antibody effector functions are modified during an immune response. Loss of all known enhancer-like elements in this region dramatically impairs CSR, but individual element deletions have no effect on this process. In the present study, we explored the hypothesis that an underlying functional redundancy in the homologous elements hs3a and hs3b was masking the importance of either element to CSR. Several transgenic mouse lines were generated, each carrying a bacterial artificial chromosome transgene that mimicked Igh locus structure but in which hs3a was missing and hs3b was flanked by loxP sites. Matings to Cyclization Recombination Enzyme-expressing mice established "pairs" of lines that differed only in the presence or absence of hs3b. Remarkably, CSR remained robust in the absence of both hs3a and hs3b, suggesting that the remaining two elements of the 3' regulatory region, hs1.2 and hs4, although individually dispensable for CSR, are, together, sufficient to support CSR.

  14. Effect of the administration time of HS6101 on hematopoietic recovery in ICR mice injured by cyclophosphamide

    Directory of Open Access Journals (Sweden)

    Shuang XING

    2015-06-01

    Full Text Available Objective To explore the effect of the administration time of HS6101 on hematopoietic recovery in ICR mice injured by cyclophosphamide (CTX. Methods One hundred and three male ICR mice were divided into 4 groups: CTX control, HS6101 prevention, HS6101 treatment, and HS6101 prevention+treatment groups. CTX was intraperitoneally injected into the ICR mice at a dose of 100mg/(kg.d for three consecutive days to establish a chemotherapeutics-injured model. HS6101 at a dose of 27μg/mouse in 0.2ml was subcutaneously injected into the mice 1h before the first administration of CTX in HS6101-prevention group, 1h after the last administration of CTX in HS6101 treatment group, and both at 1h before the first administration and 1h after the last administration of CTX in HS6101 prevention + treatment group. Physiological saline was subcutaneously injected into the mice in CTX control group (0.2ml/mouse. 10μl peripheral blood was collected from the caudal vein for WBC, neutrophil lymphocyte, RBC and platelet counts on day -1, 3, 5, 7, 9, 11, 13, 15, 17 with the MEK-7222K cell analyzer, and the cell count was compared between HS6101 treatment mice and CTX control mice. Another 30 male ICR mice were used for bone marrow colony forming unit (CFU assay and bone marrow histopathological examination, and they were assigned into normal control, CTX control, HS6101 prevention, HS6101treatment and HS6101 prevention + treatment groups (each n=6. On the day 4 and day 9 after CTX injection, mice were sacrificed and bone marrow cells were collected from the left femur for mononuclear cell (MNC isolation. 1×104 MNCs were planted in 1.0ml mouse CFU culture medium M3434 and cultured in incubator with the temperature of 37℃, and 5% CO2 for 7 days. After that, granulocyte macrophage-colony-forming unit (GM-CFU, megakaryocyte colony forming unit (MK-CFU, mixture-colony-forming unit (Mix-CFU, burst-forming unit-erythroid (BFU-E and colony-forming unit-erythroid (CFU

  15. Packing density of HS(CH2)(n)COOH self-assembled monolayers.

    Science.gov (United States)

    Snow, A W; Jernigan, G G; Ancona, M G

    2011-12-01

    Self-assembled monolayers (SAMs) of HS(CH(2))(n)COOH, n = 5, 10, 15 deposited from ethanol solution onto gold are prepared by five approaches, and their packing densities are evaluated by X-ray photoelectron spectroscopy (XPS) measurements. The five approaches are: (1) direct deposition; (2) acetic-acid-assisted deposition; (3) butyl-amine-assisted deposition; (4) displacement of a preformed HS(CH(2))(n)CH(3) (n = 5, 10, 15) SAMs; and (5) co-deposition with HS(CH(2))(n)CH(3) (n = 5, 10, 15). Packing density metrics are calculated from measurements of SAM and substrate photoemission intensities and their attenuations by two methods. In one case the attenuated photoemissions are expressed as a ratio relative to comparable measurements on an experimental HS(CH(2))(n)CH(3) model system. In the other case a new method is introduced where a calculated attenuation based on theoretical random coil and extended chain models is used as the reference to determine a packing density fraction. Packing densities are also correlated with the S2p(Au-bonded):Au4f peak area ratios and with shifts in the C1s binding energies. SAMs prepared by the direct deposition are a partial multilayer where a second molecular layer is physisorbed onto the SAM and not removable by solvent washing. The addition of acetic acid to the deposition solution disrupts dimer associations of HS(CH(2))(n)COOH in solution and at the surface of the monolayer and yields the most ordered monolayer with the highest density of -COOH groups. The addition of butyl amine results in a labile ammonium carbonate ion pair formation but results in a lower packing density in the SAM. The displacement of the preformed HS(CH(2))(n)CH(3) SAM and the co-deposition of HS(CH(2))(n)CH(3) with HS(CH(2))(n)COOH result in SAMs with little incorporation of the -COOH component.

  16. PHOTOMETRIC ANALYSIS OF HS Aqr, EG Cep, VW LMi, AND DU Boo

    Energy Technology Data Exchange (ETDEWEB)

    Djurasevic, G.; Latkovic, O. [Astronomical Observatory, Volgina 7, 11000 Belgrade (Serbia); Bastuerk, Oe.; Y Latin-Small-Letter-Dotless-I lmaz, M. [Astronomy and Space Sciences Research and Application Center, Ankara University, TR-06837 Ahlatl Latin-Small-Letter-Dotless-I bel, Ankara (Turkey); Cal Latin-Small-Letter-Dotless-I skan, S.; Senavc Latin-Small-Letter-Dotless-I , H. V.; K Latin-Small-Letter-Dotless-I l Latin-Small-Letter-Dotless-I coglu, T.; Ekmekci, F. [Department of Astronomy and Space Sciences, Faculty of Science, Ankara University, TR-06100 Tandogan, Ankara (Turkey); Tanr Latin-Small-Letter-Dotless-I verdi, T., E-mail: olivia@aob.rs [Department of Physics, Faculty of Arts and Sciences, Nigde University, 51240 Nigde (Turkey)

    2013-03-15

    We analyze new multicolor light curves for four close late-type binaries: HS Aqr, EG Cep, VW LMi, and DU Boo, in order to determine the orbital and physical parameters of the systems and estimate the distances. The analysis is done using the modeling code of G. Djurasevic, and is based on up-to-date measurements of spectroscopic elements. All four systems have complex, asymmetric light curves that we model by including bright or dark spots on one or both components. Our findings indicate that HS Aqr and EG Cep are in semi-detached, while VW LMi and DU Boo are in overcontact configurations.

  17. 2376例患儿血液hs-CRP和白细胞计数结果分析

    Institute of Scientific and Technical Information of China (English)

    梁安玉

    2014-01-01

    目的:通过分析同一血液标本中超敏C-反应蛋白(hs-CRP)、白细胞计数(WBC)检测结果及出现此结果的原因,从而为儿科临床医生诊治疾病提供有力的依据。方法:选取2376例患儿同一血液标本同时测定hs-CRP、WBC,对测定结果进行比较分析。结果:hs-CRP升高占63.8%,WBC升高占39.4%,hs-CRP和WBC均升高占29.8%,hs-CRP和WBC均正常占22.1%,hs-CRP升高而WBC正常占26.1%,hs-CRP升高而WBC降低占7.9%;hs-CRP正常而WBC降低占4.5%;hs-CRP正常而WBC升高占9.6%。结论:hs-CRP和白细胞均可作为感染的判断指标, hs-CRP更优于白细胞,hs-CRP和白细胞可出现各种不同的结果,两者结合判断更有利于患儿疾病的诊治。

  18. Hs2st mediated kidney mesenchyme induction regulates early ureteric bud branching.

    Science.gov (United States)

    Shah, Mita M; Sakurai, Hiroyuki; Sweeney, Derina E; Gallegos, Thomas F; Bush, Kevin T; Esko, Jeffrey D; Nigam, Sanjay K

    2010-03-15

    Heparan sulfate proteoglycans (HSPGs) are central modulators of developmental processes likely through their interaction with growth factors, such as GDNF, members of the FGF and TGFbeta superfamilies, EGF receptor ligands and HGF. Absence of the biosynthetic enzyme, heparan sulfate 2-O-sulfotransferase (Hs2st) leads to kidney agenesis. Using a novel combination of in vivo and in vitro approaches, we have reanalyzed the defect in morphogenesis of the Hs2st(-)(/)(-) kidney. Utilizing assays that separately model distinct stages of kidney branching morphogenesis, we found that the Hs2st(-/-) UB is able to undergo branching and induce mesenchymal-to-epithelial transformation when recombined with control MM, and the isolated Hs2st null UB is able to undergo branching morphogenesis in the presence of exogenous soluble pro-branching growth factors when embedded in an extracellular matrix, indicating that the UB is intrinsically competent. This is in contrast to the prevailing view that the defect underlying the renal agenesis phenotype is due to a primary role for 2-O sulfated HS in UB branching. Unexpectedly, the mutant MM was also fully capable of being induced in recombination experiments with wild-type tissue. Thus, both the mutant UB and mutant MM tissue appear competent in and of themselves, but the combination of mutant tissues fails in vivo and, as we show, in organ culture. We hypothesized a 2OS-dependent defect in the mutual inductive process, which could be on either the UB or MM side, since both progenitor tissues express Hs2st. In light of these observations, we specifically examined the role of the HS 2-O sulfation modification on the morphogenetic capacity of the UB and MM individually. We demonstrate that early UB branching morphogenesis is not primarily modulated by factors that depend on the HS 2-O sulfate modification; however, factors that contribute to MM induction are markedly sensitive to the 2-O sulfation modification. These data suggest that key

  19. THE CAUCHY PROBLEM OF NONLINEAR SCHR(O)DINGER-BOUSSINESQ EQUATIONS IN Hs(Rd)

    Institute of Scientific and Technical Information of China (English)

    Han Yongqian

    2005-01-01

    In this paper, the local well posedness and global well posedness of solutions for the initial value problem (IVP) of nonlinear Schrodinger-Boussinesq equations is considered in Hs(Rd) by resorting Besov spaces, where real number s ≥ 0.

  20. Genomic sequence of Campylobacter jejuni subsp. jejuni HS:19 Penner serotype reference strain RM3420

    NARCIS (Netherlands)

    C.T. Parker (Craig); Huynh, S. (Steven); A.P. Heikema (Astrid)

    2017-01-01

    textabstractCampylobacter jejuni subsp. jejuni infections are a leading cause of foodborne gastroenteritis and the most prevalent antecedent to Guillain-Barré syndrome (GBS). Penner serotype HS:19 is among several capsular types shown to be markers for GBS. This study describes the genome of C.

  1. Baseline distribution and correlation analysis of hsCRP in an insurance applicant population.

    Science.gov (United States)

    Krause, Kenneth J; Williams, David S; White, Nancy

    2008-01-01

    Many clinical studies have shown that baseline levels of high sensitivity C-reactive protein (hsCRP) in apparently healthy men and women are highly predictive of future risk of heart attack, stroke, sudden cardiac death, and the development of peripheral arterial disease. This paper presents an analysis of the baseline characteristics of our prospective study cohort. The intent of our prospective study is to determine whether hsCRP can be used to better classify risk for life insurance applicants already at risk for cardiovascular events, as well as those who are not. The possibility that low levels of hsCRP levels in otherwise healthy applicants might be associated with more favorable cardiovascular risk could allow this test to be used to more precisely stratify risk in the standard-or-better underwriting classifications. In this preliminary analysis, high sensitivity CRP appears to be weakly correlated with BMI, and perhaps triglyceride level in this cohort of insurance applicants. Somewhat surprisingly, in contrast to many published reports, hsCRP was not found to be correlated with other lipid measures (TC, HDL, LDL), dysmetabolic markers or smoking classification during the underwriting process. We plan to analyze mortality results as they evolve in the future.

  2. The Role of the Media in South African Construction Health and Safety (H&S

    Directory of Open Access Journals (Sweden)

    John Smallwood

    2012-11-01

    Full Text Available A large number of fatalities and injuries occur in the South African construction industry. Traditionally, the print media have dedicated editorial, published news, articles and letters, and have exposed abusive or non-conforming conditions and practices in terms of H&S. Literature also indicates that the print media can influence and has an impact on H&S.Given the level of fatalities and injuries and the potential role of the print media, a postal survey was conducted among editors of construction and related magazines. Findings indicate that: the print media do contribute to and play a role in construction H&S; industry has the capacity and needs to promote H&S on a wider basis; there is a need to improve construction H&S; to a degree, editors are aware of what constitutes unsafe acts and unsafe conditions, and the print media can play an increased role through the review of articles, advertisements, advertorial, editorial and phototgraphs to prevent the depiction of unhealthy and unsafe practices and conditions

  3. Synthetic Human β-Globin 5'HS2 Constructs Function as Partially Active Locus Control Regions.

    NARCIS (Netherlands)

    J. Ellis (James); D. Talbot; N.O. Dillon (Niall); F.G. Grosveld (Frank)

    1993-01-01

    textabstractTransgenes linked to the beta-globin locus control region (LCR) are transcribed in a copy-dependent manner that is independent of the integration site. It has previously been shown that the LCR 5'HS2 region does not require its NF-E2 dimer binding site for LCR activity. In this paper we

  4. Amygdalin Regulates Apoptosis and Adhesion in Hs578T Triple-Negative Breast Cancer Cells.

    Science.gov (United States)

    Lee, Hye Min; Moon, Aree

    2016-01-01

    Amygdalin, D-mandelonitrile-β-D-glucoside-6-β-glucoside, belongs to aromatic cyanogenic glycoside group derived from rosaceous plant seed. Mounting evidence has supported the anti-cancer effects of amygdalin. However, whether amygdalin indeed acts as an anti-tumor agent against breast cancer cells is not clear. The present study aimed to investigate the effect of amygdalin on the proliferation of human breast cancer cells. Here, we show that amygdalin exerted cytotoxic activities on estrogen receptors (ER)-positive MCF7 cells, and MDA-MB-231 and Hs578T triple-negative breast cancer (TNBC) cells. Amygdalin induced apoptosis of Hs578T TNBC cells. Amygdalin downregulated B-cell lymphoma 2 (Bcl-2), upregulated Bcl-2-associated X protein (Bax), activated of caspase-3 and cleaved poly ADP-ribose polymerase (PARP). Amygdalin activated a pro-apoptotic signaling molecule p38 mitogen-activated protein kinases (p38 MAPK) in Hs578T cells. Treatment of amygdalin significantly inhibited the adhesion of Hs578T cells, in which integrin α5 may be involved. Taken together, this study demonstrates that amygdalin induces apoptosis and inhibits adhesion of breast cancer cells. The results suggest a potential application of amygdalin as a chemopreventive agent to prevent or alleviate progression of breast cancer, especially TNBC.

  5. Synergistic Activity of the Plant Defensin HsAFP1 and Caspofungin against Candida albicans Biofilms and Planktonic Cultures.

    Directory of Open Access Journals (Sweden)

    Kim Vriens

    Full Text Available Plant defensins are small, cysteine-rich peptides with antifungal activity against a broad range of yeast and fungi. In this study we investigated the antibiofilm activity of a plant defensin from coral bells (Heuchera sanguinea, i.e. HsAFP1. To this end, HsAFP1 was heterologously produced using Pichia pastoris as a host. The recombinant peptide rHsAFP1 showed a similar antifungal activity against the plant pathogen Fusarium culmorum as native HsAFP1 purified from seeds. NMR analysis revealed that rHsAFP1 consists of an α-helix and a triple-stranded antiparallel β-sheet stabilised by four intramolecular disulfide bonds. We found that rHsAFP1 can inhibit growth of the human pathogen Candida albicans as well as prevent C. albicans biofilm formation with a BIC50 (i.e. the minimum rHsAFP1 concentration required to inhibit biofilm formation by 50% as compared to control treatment of 11.00 ± 1.70 μM. As such, this is the first report of a plant defensin exhibiting inhibitory activity against fungal biofilms. We further analysed the potential of rHsAFP1 to increase the activity of the conventional antimycotics caspofungin and amphotericin B towards C. albicans. Synergistic effects were observed between rHsAFP1 and these compounds against both planktonic C. albicans cells and biofilms. Most notably, concentrations of rHsAFP1 as low as 0.53 μM resulted in a synergistic activity with caspofungin against pre-grown C. albicans biofilms. rHsAFP1 was found non-toxic towards human HepG2 cells up to 40 μM, thereby supporting the lack of a general cytotoxic activity as previously reported for HsAFP1. A structure-function study with 24-mer synthetic peptides spanning the entire HsAFP1 sequence revealed the importance of the γ-core and its adjacent regions for HsAFP1 antibiofilm activity. These findings point towards broad applications of rHsAFP1 and its derivatives in the field of antifungal and antibiofilm drug development.

  6. The Use of Postoperative Serum HS-AFP and GGT Ⅱ for Judgment of the Prognosis for Hepatocellular Carcinoma patients

    Institute of Scientific and Technical Information of China (English)

    Runzhou Ni; Lei Yang; Mingbing Xiao; Feng Li; Cuihua Lu

    2006-01-01

    OBJECTIVE To investigate the clinical value of hepatoma-specific alpha-fetoprotein (HS-AFP) and gamma-glutamyltransferase Ⅱ (GGT Ⅱ) for judgment of postoperative prognosis of patients with hepatocellular carcinoma (HCC).METHODS HS-AFP was separated and determined using native polyacrylamide electrophoresis with a discontinuous buffer system and Western blots. GGT Ⅱ was separated with native polyacrylamide electrophoresis with a discontinuous buffer system and detected by enzyme staining. Forty cases with HCC underwent serial determination of HS-AFP and GGT Ⅱ before and after radical excision. The correlations were analyzed between the two indices and survival time.RESULTS In the 40 cases with HCC, before radical excision the positive rates of HS-AFP and GGT Ⅱ were 57.5% and 67.5% respectively, with the positive rate of combined HS-AFP and GGT Ⅱ reaching 80.0%. After operation, the recurrence and metastasis rate in the groups with positive HS-AFP and GGT Ⅱ were 90.9% and 58.8% respectively, while in the groups with negative HS-AFP and GGT Ⅱ the rates were 20.7% and 26.1% respectively. Recurrence and metastasis occurred in all cases with both postoperative positive HS-AFP and GGT Ⅱ but only in 9.5% of the cases in whom both postoperative HS-AFP and GGT Ⅱ were negative. Univariate analysis revealed that postoperative HS-AFP and GGT Ⅱ were related to the prognosis in HCC.CONCLUSION Postoperative serum HS-AFP and GGT Ⅱ are very useful in predicting the prognosis of HCC patients.

  7. Comparative analysis of the aroma chemicals of Melissa officinalis using hydrodistillation and HS-SPME techniques

    Directory of Open Access Journals (Sweden)

    Shakeel-u- Rehman

    2017-05-01

    Full Text Available Headspace solid-phase micro extraction (HS-SPME coupled with gas chromatography–mass spectrometry (GC–MS has been used for the chemical analysis of Melissa officinalis (leaves cultivated in Institute Germplasm. The HS-SPME analysis led to the identification of 22 components constituting 99.1% of the total volatile constituents present in the leaves whereas its hydrodistillate led to the identification of 24 volatile constituents constituting 98.1% of the volatile material. The chemical composition of the SPME and hydrodistilled extract of M. officinalis leaves comprised mainly of oxygenated monoterpenes (78.5% and 57.8% respectively and sesquiterpene hydrocarbons (14.9% and 29.7% respectively. The major components identified in the HS-SPME extract were citronellal (31.1%, citronellol (18.3%, β-caryophyllene (12.0%, (E-citral (11.9%, (Z-citral (9.6%, geraniol (3.6%, (Z-β-ocimene (3.1% and 1-octen-3-ol (2.0% whereas hydrodistilled essential oil was rich in (Z-citral (19.6%, β-caryophyllene (13.2%, (E-citral (11.2%, citronellal (10.2%, germacrene-d (8.3%, δ-3-carene (5.0%, 6-methyl-5-hepten-2-one (3.7% and citronellyl acetate (3.7%. The comparative analysis of volatile constituents of M. officinalis leaf extract using HS-SPME and hydrodistillation techniques shows both qualitative as well as quantitative differences. The current study is the first report involving rapid analysis of volatile components of M. officinalis by HS-SPME.

  8. Post-synthetic regulation of HS structure: the yin and yang of the Sulfs in Cancer

    Directory of Open Access Journals (Sweden)

    Romain R Vives

    2014-01-01

    Full Text Available Heparan sulfate (HS is a complex polysaccharide that takes part in most major cellular processes, through its ability to bind and modulate a very large array of proteins. These interactions involve saccharide domains of specific sulfation pattern (S-domains, the assembly of which is tightly orchestrated by a highly regulated biosynthesis machinery. Another level of structural control does also take place at the cell surface, where degrading enzymes further modify HS post-synthetically. Amongst them are the Sulfs, a family of extracellular sulfatases (two isoforms in human that catalyze the specific 6-O-desulfation of HS. By targeting HS functional sulfated domains, Sulfs dramatically alter its ligand binding properties, thereby modulating a broad range of signaling pathways. Consequently, Sulfs play major roles during development, as well as in tissue homeostasis and repair. Sulfs have also been associated with many pathologies including cancer, but despite increasing interest, the role of Sulfs in tumor development still remains unclear. Studies have been hindered by a poor understanding of the Sulf enzymatic activities and conflicting data have shown either anti-oncogenic or tumor-promoting effects of these enzymes, depending on the tumor models analyzed. These opposite effects clearly illustrate the fine tuning of HS functions by the Sulfs, and the need to clarify the mechanisms involved. In this review, we will detail the present knowledge on the structural and functional properties of the Sulfs, with a special focus on their implication during tumor progression. Finally, we will discuss attempts and perspectives of using the Sulfs as a biomarker of cancer prognosis and diagnostic and as a target for anti-cancer therapies.

  9. The diverse application of laser hair removal therapy: a tertiary laser unit's experience with less common indications and a literature overview.

    Science.gov (United States)

    Koch, D; Pratsou, P; Szczecinska, W; Lanigan, S; Abdullah, A

    2015-01-01

    We describe the diversity of indications for laser hair removal (LHR) therapy and compare our experience with the literature. Patients' case notes referred to the Birmingham Regional Skin Laser Centre between 2003 and 2011 for laser hair removal, with indications other than hirsutism, were reviewed retrospectively. Thirty-one treated patients with the following indications were identified: hair-bearing skin grafts/flaps, intra-oral hair-bearing flap, Becker's naevus, localised nevoid hypertrichosis, peristomal hair-bearing skin, scrotal skin prior to vaginoplasty in male-to-female (MTF) gender reassignment, pilonidal sinus disease (PSD), pseudofolliculitis barbae (PFB) and hidradenitis suppurativa (HS). Seven patients with the following indications have been reported before: intra-oral hair-bearing graft, naevoid hypertrichosis and peristomal hair-bearing skin. A clinical review of the evidence available for each indication is provided. Our experience and that in the published literature suggest that LHR is a safe, well-tolerated and effective treatment modality for the indications we report, leading to significant symptom and functional improvement with high patient satisfaction. LHR appears effective in the treatment of chronic inflammatory conditions such as PSD, PFB and HS, particularly at an early disease stage. We aim to increase awareness of the diversity of laser hair removal indications and add evidence to the medical literature of the wide range of indications for this useful treatment modality.

  10. Unstable Angina Pectoris Patients Serum hs-CRP Levels in Clinical Research%不稳定心绞痛患者血清hs-CRP水平临床研究

    Institute of Scientific and Technical Information of China (English)

    易慧芳

    2014-01-01

    Objective Investigate al ergic hs CRP determination in patients with unstable angina pectoris diagnosis value of serum. Methods Choose 60 cases of unstable angina pectoris patients as observation group, In the treatment of day 1, day 15 test hs-CRP concentration in serum, select 60 outpatient patients with stable angina pectoris patients as control group. Results Group of patients with unstable angina pectoris patients serum hs- CRP concentration significantly increased, compared with the control group with very significant dif erence( P<0.05 ) ,Observation group in the treatment of before and after comparison, hs-CRP concentration in serum increased obviously, there are significant dif erences(P<0.05). Conclusion The hs-CRP significantly higher in patients with unstable angina pectoris, has a high diagnostic value.%目的:探讨超敏C反应蛋白( High sensitivity C Reactive Protein,hs-CRP )测定在不稳定心绞痛患者血清中的诊断价值。方法选取60例不稳定心绞痛患者作为观察组,在入院第1d、第15d检测血清中hs-CRP浓度,选取门诊稳定心绞痛患者60例作为对照组。结果不稳定心绞痛患者患者组血清中hs-CRP浓度明显增高,与对照组比较有非常显著性差异(P<0.05),观察组在治疗前、后比较,血清中hs-CRP浓度明显增高,有显著性差异(P<0.05)。结论 hs-CRP在不稳定心绞痛患者中显著增高,有很高的诊断价值。

  11. ROS-Dependent Neuroprotective Effects of NaHS in Ischemia Brain Injury Involves the PARP/AIF Pathway

    Directory of Open Access Journals (Sweden)

    Qian Yu

    2015-07-01

    Full Text Available Background/Aims: Stroke is among the top causes of death worldwide. Neuroprotective agents are thus considered as potentially powerful treatment of stroke. Methods: Using both HT22 cells and male Sprague-Dawley rats as in vitro and in vivo models, we investigated the effect of NaHS, an exogenous donor of H2S, on the focal cerebral ischemia-reperfusion (I/R induced brain injury. Results: Administration of NaHS significantly decreased the brain infarcted area as compared to the I/R group in a dose-dependent manner. Mechanistic studies demonstrated that NaHS-treated rats displayed significant reduction of malondialdehyde content, and strikingly increased activity of superoxide dismutases and glutathione peroxidase in the brain tissues compared with I/R group. The enhanced antioxidant capacity as well as restored mitochondrial function are NaHS-treatment correlated with decreased cellular reactive oxygen species level and compromised apoptosis in vitro or in vivo in the presence of NaHS compared with control. Further analysis revealed that the inhibition of PARP-1 cleavage and AIF translocation are involved in the neuroprotective effects of NaHS. Conclusion: Collectively, our results suggest that NaHS has potent protective effects against the brain injury induced by I/R. NaHS is possibly effective through inhibition of oxidative stress and apoptosis.

  12. Elevated Preoperative Serum Hs-CRP Level as a Prognostic Factor in Patients Who Underwent Resection for Hepatocellular Carcinoma.

    Science.gov (United States)

    Liu, Yu-Bin; Ying, Jie; Kuang, Su-Juan; Jin, Hao-Sheng; Yin, Zi; Chang, Liang; Yang, Hui; Ou, Ying-Liang; Zheng, Jiang-Hua; Zhang, Wei-Dong; Li, Chuan-Sheng; Jian, Zhi-Xiang

    2015-12-01

    To evaluate the effects of preoperative highly sensitive C-reactive protein (Hs-CRP) in serum on the prognostic outcomes of patients with hepatocellular carcinoma (HCC) following hepatic resection in Chinese samples.From January 2004 to December 2008, a total of 624 consecutive HCC patients who underwent hepatic resection were incorporated. Serum levels of Hs-CRP were tested at preoperation via a collection of venous blood samples. Survival analyses adopted the univariate and multivariate analyses.In our study, among the 624 screened HCC patients, 516 patients were eventually incorporated and completed follow-up. Positive correlations were found regarding preoperative serum Hs-CRP level and tumor size, Child-Pugh class, or tumor stage (all P Hs-CRP levels at preoperation (both P Hs-CRP-normal group, the overall survival (OS) and recurrence-free survival rates were evidently decreased in the Hs-CRP-elevated group. Further, preoperative serum Hs-CRP level might be having possible prediction effect regarding survival and recurrence of HCC patients after hepatic section in the multivariate analysis.Preoperative increased serum Hs-CRP level was an independent prognostic indicator in patients with HCC following hepatic resection in Chinese samples.

  13. Correlation of Serum Levels of Vitronectin, Malondialdehyde and Hs-CRP With Disease Severity in Coronary Artery Disease

    Directory of Open Access Journals (Sweden)

    Alireza Yaghoubi

    2015-09-01

    Conclusion: The association and correlation between VN, MDA and hs-CRP indicate their involvement in the atherosclerosis process that may lead to progression of CAD. Also, these findings suggested that serum levels of VN, MDA and hs-CRP can help as diagnostic and monitoring markers in CAD patients and as markers of disease severity.

  14. The dependence of the blood level of the oxime HS 6 on the severity of organophosphate poisoning

    NARCIS (Netherlands)

    Wolthuis, O.L.; Clason-van der Wiel, H.J.; Visser, R.P.L.S.

    1976-01-01

    Atropinised anaesthetised rats were injected i.v. with 4, 6 or 8 x LD50 of the organophosphorous anticholinesterase soman. Subsequent treatment with one dose of HS 6 (100 mg/kg, i.v.) delayed respiratory failure by 1 h or more; a further postponement was obtained when an additional HS 6 infusion was

  15. Hematopoietic lineage cell specific protein 1 (HS1) is a functionally important signaling molecule in platelet activation.

    Science.gov (United States)

    Kahner, Bryan N; Dorsam, Robert T; Mada, Sripal R; Kim, Soochong; Stalker, Timothy J; Brass, Lawrence F; Daniel, James L; Kitamura, Daisuke; Kunapuli, Satya P

    2007-10-01

    Collagen activates platelets through an intracellular signaling cascade downstream of glycoprotein VI (GPVI). We have investigated the contribution of hematopoietic lineage cell-specific protein 1 (HS1) downstream of GPVI in platelet activation. Stimulation of GPVI leads to tyrosine phosphorylation of HS1, which is blocked by Src-family kinase inhibitors. Coimmunoprecipitation experiments revealed that HS1 associates with Syk and phosphatidylinositol 3-kinases. HS1-null mice displayed increased bleeding times and increased time to occlusion in the FeCl(3) in vivo thrombosis model compared with their wild-type littermates. In addition, aggregation and secretion responses were diminished in HS1-null mouse platelets after stimulation of GPVI and protease-activated receptor 4 (PAR-4) agonists compared with wild-type littermate mouse platelets. Finally, Akt phosphorylation was diminished after GPVI or PAR-4 stimulation in platelets from HS1-null mice compared with their wild-type littermates. These results demonstrate that phosphorylation of the HS1 protein occurs downstream of GPVI stimulation and that HS1 plays a significant functional role in platelet activation downstream of GPVI and PARs.

  16. Proteomic Comparison of Fruit Ripening between 'Hedelfinger' Sweet Cherry (Prunus avium L.) and Its Somaclonal Variant 'HS'.

    Science.gov (United States)

    Prinsi, Bhakti; Negri, Alfredo S; Espen, Luca; Piagnani, M Claudia

    2016-05-25

    The somaclonal variant HS, from sweet cherry (Prunus avium L.) 'Hedelfinger' (H), was previously selected for reduced tree vegetative vigor and lesser canopy density. In this work, we compared H and HS fruits at early unripe (green) and full ripe (dark red) stages by biochemical and proteomic approaches. The main biochemical parameters showed that fruit quality was not affected by somaclonal variation. The proteomic analysis identified 39 proteins differentially accumulated between H and HS fruits at the two ripening stages, embracing enzymes involved in several pathways, such as carbon metabolism, cell wall modification, stress response, and secondary metabolism. The evaluation of fruit phenolic composition by mass spectrometry showed that HS sweet cherries have higher levels of procyanidin, flavonol, and anthocyanin compounds. This work provides the first proteomic characterization of fruit ripening in sweet cherry, revealing new positive traits of the HS somaclonal variant.

  17. Analysis of the changes of serum lipid and hs-CRP in patients with subclinical hypothyroidism

    Institute of Scientific and Technical Information of China (English)

    Jin-Mian Fan; Dong-Bo Zheng; Zhou-Ping Zhang

    2016-01-01

    Objective:To analyze changes of serum lipid and hs-CRP levels in patients with subclinical hypothyroidism in order to explore their correlations with TSH level.Methods:A total of 90 patients with subclinical hypothyroidism were included in the study and divided into the mild, moderate, and severe groups according to TSH level with 30 cases in each group; meanwhile, 30 healthy individuals were served as the control groups. The changes of the levels of TSH, serum lipid indicators (TC, TG, HDL-C, LDL-C, ApoA, ApoB, and Lpa), and hs-CRP in the four groups were observed. The correlation of serum lipid and hs-CRP with TSH was analyzed. Results: The levels of TSH, TC, TG, Lpa, and hs-CRP in the subclinical hypothyroidism subgroups were significantly higher than those in the control group. The levels of HDL-C, LDL-C, ApoA, and ApoB in patients with subclinical hypothyroidism were increased when compared with the control group. The levels of HDL-C, LDL-C, and ApoB in the moderate and severe groups were significantly higher than those in the control group. ApoA level in the severe group was significantly higher than that in the control group. LDL-C in the moderate group was significantly higher than that in the mild group, and that in the severe group was significantly higher than that in the moderate group. ApoB level in the severe group was significantly higher than that in the mild group. Spearman correlation analysis showed that TC, TG, LDL-C, ApoB, Lpa, and hs-CRP were positively correlated with TSH level, while HDL-C and ApoA had no correlation with TSH level.Conclusions:The levels of serum lipid and hs-CRP are abnormally elevated in patients with subclinical hypothyroidism, and are gradually increased along with the disease progression, which is closely associated with TSH level.

  18. hsCRP in pre-hypertension and hypertension: a prospective study in Southern Asian region

    Directory of Open Access Journals (Sweden)

    Satwika Sinha

    2014-08-01

    Full Text Available Background: Hypertension is turned into a leading cause of non-communicable disease associated mortality and morbidity in both developing as well as developed world. Hypertension is reported to be the fourth contributor to premature death in developed countries and the seventh in developing countries. In the regard of early diagnosis and better prognosis, the concept of pre-hypertension, defined as a systolic blood pressure of 120-139 mmHg and/or a diastolic blood pressure of 80-89 mmHg was introduced as the new guideline for the management of blood pressure by the seventh report of the Joint National Committee on prevention, detection, evaluation, and treatment of high blood pressure (JNC-7. Among various other factors inflammation may be a causative factor for development of Hypertension But the association is not very clear. Accordingly, we have designed our study to find any association of hsCRP with pre-hypertension and hypertension so that early prevention and control can help to avoid or delay the grave outcome and complications of hypertension. Methods: A total of 37 hypertensives, 30 pre-hypertensives and 31 age and sex matched healthy control subjects were selected for the study, with consent. Two BP readings were taken five minutes apart, on both arms, with a mercury sphygmomanometer. The estimation of serum hsCRP was done on XL-600 automatic analyzer with the kit (Erba Mannheim based on the measurement of antigen-antibody reaction by the end-point method. Results: There is significant difference in systemic and diastolic blood pressure and hsCRP in between group study. In pre-hypertensive group hsCRP is correlated with diastolic blood pressure. Conclusion: Our results suggest a correlation exists between hsCRP and hypertension more significantly with pre-hypertension. So estimation of serum hsCRP can be a good diagnostic as well as prognostic marker in diagnosing pre-hypertensives and prevent the occurance of hypertension and cardio

  19. Performance Analysis of an Enhanced PRMA-HS Protocol for LEO Satellite Communication

    Institute of Scientific and Technical Information of China (English)

    ZHUO Yong-ning; YAN Shao-hu; WU Shi-qi

    2005-01-01

    The packet reservation multiple access with hindering state (PRMA-HS) is a protocol suitable for LEO satellite mobile communication. Although working well with light system payload (amount of user terminals), the protocol imposes high channel congestion on system with heavy payload, thus degrades the system's quality of service. To controlling the channel congestion, a scheme of enhanced PRMA-HS protocol is proposed, which aims to reduce the collision of voice packets by adopting a mechanism of access control. Through theoretic analysis, the system's mathematic model is presented and the packet drop probability of the scheme is deduced. To testify the performance of the scheme, a simulation is performed and the results support our analysis.

  20. The e-NutriHS: a web-based system for a Brazilian cohort study.

    Science.gov (United States)

    Folchetti, Luciana D; da Silva, Isis T; de Almeida Pititto, Bianca; Ferreira, Sandra R G

    2015-01-01

    The e-NutriHS is a web-based system developed to gather online information on health of a cohort of college students and graduates in nutrition. It consists of six validated and internationally recognized questionnaires regarding demographic and socioeconomic data, dietary habits, physical activity level, alcohol and tobacco use, anti-fat attitudes and personal and family histories. Our software and respective database is hosted in the School of Public Health server and is based on free programming languages. An e-NutriHS prototype was created preceding online deployment. An improved version of the website was released based on 20 volunteers' opinions. A total of 503 users were registered. Considering that web-based systems produce reliable data, are easy to use, less costly and are less time-consuming, we conclude that our experience deserves to be shared, particularly with middle income economy countries.

  1. Sulfur isotope effects associated with protonation of HS- and volatilization of H2S

    Science.gov (United States)

    Fry, B.; Gest, H.; Hayes, J. M.

    1986-01-01

    The isotope effects associated with: (1) formation of H2S from HS- by protonation in aqueous solution; and (2) volatilization of H2S have been experimentally determined. Both isotopic distributions in closed systems at equilibrium and differential rates of volatilization of isotopic species in open systems were measured at 22 +/- 1 degrees C. It was found that, at equilibrium aqueous H2S is enriched in 34S by 2.0 - 2.7% relative to HS- and that H2S volatilized from solution is depleted in 34S by 0.5% relative to dissolved H2S. A small kinetic isotope effect accompanying volatilization of H2S was observed in the open-system experiments.

  2. Sucrose Octaacetate Chemical Kinetics and Shelf Lives at Various Formulation pHs.

    Science.gov (United States)

    Gaddam, Shalini; Stagner, William C

    2017-06-23

    Developing pediatric friendly dosage forms is a high priority worldwide. Sucrose octaacetate (SOA) has been recommended for use as a surrogate for bitter tasting active pharmaceutical ingredients. Even though SOA has found a number of human use applications and has been employed for decades, there are no rigorous chemical kinetic studies reported. A recently reported SOA stability-indicating method was used to perform SOA chemical kinetic and stability studies. As part of the chemical kinetic study, reaction order, activation energies, extrapolated rate constants, pH-rate profiles at 4 and 25°C, and estimated shelf lives at 4 and 25°C at different buffer pHs are provided. The estimated SOA shelf lives at 25°C and pHs 4.00, 5.20, and 6.00 were 25.3, 114, and 27.4 days, respectively. At 4°C, SOA's estimated shelf lives were 0.478, 5.26, and 1.47 years at pHs 4.00, 5.20, and 6.00, respectively. SOA can be formulated at pHs 4 to 6 and stored at 25°C for short-duration (less than 25 days) uses such as a bitter tasting surrogate for fundamental taste mechanism studies or brief taste masking assessment clinical studies. For longer term solution studies, like being used as a bitter tasting control for blinded clinical trials, SOA should be formulated at the optimum pH of 5.40 and refrigerated at 4°C for maximum stability. The reported data can be used as a starting point for developing stable SOA formulations and estimating shelf life.

  3. A New Compound Along With Seven Known Compounds from an Endophytic Fungus Aspergillus sp. HS-05

    Directory of Open Access Journals (Sweden)

    Gang Chen

    2013-08-01

    Full Text Available Investigation of EtOAc extract from the fermentation broth of the endophytic fungus Asperilligus sp. HS-05 led to the isolation of a new compound (1 of spiro moiety named aspergispiroketal and seven known compounds (2-8. Their structures were elucidated mainly by NMR and HR-TOF-MS, as well as on comparison with the reported data. The absolute configuration of 1 was defined by comparison of quantum chemical TDDFT calculated and experimental ECD spectra.

  4. Linear and Nonlinear Optical Characterization of PolyDCHD-HS Films

    Institute of Scientific and Technical Information of China (English)

    CHEN Xianfeng; XIE Shengwu; CHEN Yingli; XIA Yuxin; S. Stefano; E. Giorgetti; G. Margatti

    2000-01-01

    Waveguiding films of the highly soluble polycarbazolydiacetylene polyDCHD-HS were prepared by spin coating and characterized at different wavelengths: 5 dB/cm propagation losses were observed at 1321 nm.Measurements of the third order nonliearity at 1064nm with the surface plasmon resonance method gave the valueχ(3)= i4.4×10-17+3.5×10-18 m2/V2.

  5. Right-invariant Sobolev metrics ${H}^{s}$ on the diffeomorphisms group of the circle

    CERN Document Server

    Escher, Joachim

    2012-01-01

    We study the geodesic flow on the diffeomorphisms group of the circle with respect to the right-invariant metric induced by the fractional Sobolev norm $H^s$ for $s\\ge1/2$. We show that the corresponding initial value problem possesses a maximal solution in the smooth category and that the Riemannian exponential mapping is a smooth diffeomorphism from a neighbourhood of 0 in $C^{\\infty}(S)$ onto a neighbourhood of the identity in $Diff^{\\infty}(S)$.

  6. Repeatability Using Automatic Tracing with Canon OCT- HS100 and Zeiss Cirrus HD-OCT 5000

    OpenAIRE

    Rune Brautaset; Ulrika Birkeldh; Petra Frehr Alstig; Petra Wikén; Maria Nilsson

    2016-01-01

    Background Optical coherence tomography (OCT), can be used in clinical practice to provide high resolution cross-sectional images of the retina, optic disc and macula structure. These measurements can be useful for early detection, diagnosis, monitoring and treatment guidance for retinal diseases. Therefore, repeatability of measurements in OCT is of great importance. Methods Macula and optic disc parameters from the right eye of 30 healthy subjects were obtained twice with the Canon OCT-HS10...

  7. 慢性心力衰竭患者血清MMP-9和hs-CRP的表达%Expressions of serum MMP-9 and hs-CRP in patients with chronic heart failure

    Institute of Scientific and Technical Information of China (English)

    郑利平

    2012-01-01

    Objective To investigate the changes of serum matrix metalloproteinase-9(MMP-9) and high sensitive Creactive protein(hs-CRP) expressions in the patients with chronic heart failure (CHF). Methods A total of 120 CHF patients was divided into 4 groups according to NYHA cardiac function classes of Ⅰ ,Ⅱ , Ⅲ and Ⅳ. The expression levels of MMP-9 and hs-CRP were detected with ELISA and Latex enhanced immunoturbidimetric assay, respectively, and compared among 4 NYHA cardiac cardiac function classes. Results The expression levels of MMP-9 and hs-CRP were increased as the cardiac function became worsen(P<0. 05). The expression of serum MMP-9 was positively correlated to that of hs-CRP(r=0. 716,P<0. 01). Conclusion When the cardiac dysfunction becomes severe in CHF patients,the expressions of MMP-9 and hs-CRP are remarkably increased, which may participate in the myocardial remodeling of CHF.%目的 探讨基质金属蛋白酶9(MMP-9)和高敏C反应蛋白(hs-CRP)在慢性心力衰竭(CHF)患者的表达.方法 CHF患者120例,ELISA检测血清MMP-9表达,乳胶增强免疫比浊法检测血清hs-CRP水平.比较不同NYHA心功能状态患者MMP-9和hs-CRP的表达差异.结果 随着心功能的恶化,MMP-9和hs-CRP的表达水平显著升高(P<0.05).CHF患者外周血MMP-9和hs-CRP的表达水平呈正相关(r=0.716,P<0.01).结论 CHF患者外周血MMP-9和hs-CRP表达水平随着心功能的减低而升高,MMP-9和hs-CRP表达可能参与了CHF心肌重构的病理过程.

  8. Interference of anesthetics in blood alcohol analysis by HS-GC-FID: A case report.

    Science.gov (United States)

    Monteiro, C; Proença, P; Tavares, C; Castañera, A; Corte Real, F

    2016-08-01

    One of the techniques most widely used in ethanol analysis in forensic laboratories is undoubtedly the headspace gas chromatography with flame-ionization detection (HS-GC-FID) since the determination of this substance is carried out directly, without the need for additional purification procedures, which leads to increased productivity. This is a very important factor due to the high number of alcohol analysis requested to these laboratories. The presence of other volatile substances can cause a problem given the fact that they can be interferents in ethanol analysis by HS-GC-FID, which can have legal consequences related with driving under the influence of alcohol. The authors report a case of a routine analysis by HS-GC-FID for the determination of ethanol of a driver who has suffered an accident in which the use of two chromatographic columns with different polarities was essential to obtain an unequivocally identification of this substance in presence of an interfering volatile anesthetic administered in the hospital. The method was validated according to international recommendations before being introduced into routine laboratory in terms of selectivity, limits of detection (LOD), limits of quantification (LOQ), linearity, repeatability, intermediate precision, accuracy, robustness and carryover.

  9. Infrared Studies of the Reflective Properties of Solar Cells and the HS376 Spacecraft

    Science.gov (United States)

    Frith, James; Reyes, Jacqueline; Cowardin, Heather; Anz-Meador, Phillip; Buckalew, Brent; Lederer, Susan

    2016-01-01

    In 2015, a selection of HS-376 buses were observed photometrically with the United Kingdom Infrared Telescope (UKIRT) to explore relationships between time-on-orbit and Near Infrared (NIR) color. These buses were chosen because of their relatively simple shape, for the abundance of similar observable targets, and their surface material being primarily covered by solar cells. While the HS-376 spacecraft were all very similar in design, differences in the specific solar cells used in the construction of each model proved to be an unconstrained variable that could affect the observed reflective properties. In 2016, samples of the solar cells used on various models of HS-376 spacecraft were obtained from Boeing and were analyzed in the Optical Measurements Center at the Johnson Space Center using a visible-near infrared field spectrometer. The laboratory-based spectra are convolved to match the photometric bands previously obtained using UKIRT and compared with the on-orbit photometry. The results and future work are discussed here.

  10. Correlation of Progranulin, Granulin, Adiponectin and Vaspin with Metaflammation (hs-CRP in Indonesian Obese Men

    Directory of Open Access Journals (Sweden)

    Rosalia E Napitupulu

    2013-08-01

    Full Text Available BACKGROUND: Obesity is closely related to chronic, low grade systemic inflammation (metaflammation and it leads to further metabolic complications such as hypertension, atherosclerosis, and type 2 diabetes due to the adipocytokine imbalance. This study was carried out to assess the correlation between progranulin, granulin, adiponectin and visceral adipose tissue-derived serine protease inhibitor (Vaspin with metaflammation (high sensitivity C-reactive protein (hs-CRP in centrally obese men. METHODS: This study was observational with a cross sectional design involving 60 men aged 30-60 years, consisted of 43 obese men (waist circumference (WC ≥90 cm and 13 non obese men (WC 105 cm. CONCLUSIONS: We found metaflammation (hs-CRP was significantly correlated with Vaspin, but not with progranulin, granulin and adiponectin, in obese men. We suggest the possibility of a dynamic expression of adipokines related to WC that are subjected to adipocytes hypertrophy-hyperplasia phenomenon. KEYWORDS: progranulin, granulin, adiponectin, Vaspin, hs-CRP, metaflammation, central obesity.

  11. Correlation of hs-CRP with environmental risk factors of nephropathy in type 2 diabetes

    Directory of Open Access Journals (Sweden)

    Jay Prakash Sah

    2015-06-01

    Full Text Available The objective of the present study was to investigate the association of hs-CRP levels with environmental risk factors of diabetic nephropathy like smoking, drinking alcohol, diet, age of diabetic patient, duration of diabetes, medication of diabetes, and blood pressure medication. A hospital-based quantitative study was conducted at the Department of Clinical Biochemistry of Manipal Teaching Hospital (MTH Pokhara, Nepal, with 89 patients suffering from type 2 diabetes. Blood samples (n=89 from the patients were collected and the serums were separated. On the other hand, data on environmental risk factors of nephropathy were collected by using standard questionnaire. In this study, serum hs-CRP level was not found to be correlated with smoking (p=0.111, alcohol consumption (p=0.722, diet (p=0.496, duration of diabetes (p=0.519, age of diabetic patient (p=0.369, medication of diabetes (p=0.734, and blood pressure medication (p=0.625. Hence, our study concludes that serum hs-CRP value in type 2 diabetic patients is insignificantly correlated with the risk factors especially smoking, drinking alcohol, diet, duration of diabetes, age of diabetic patient, medication of diabetes, and medication of blood pressure.

  12. Hyperphosphatemia and hs-CRP Initiate the Coronary Artery Calcification in Peritoneal Dialysis Patients.

    Science.gov (United States)

    Shang, Da; Xie, Qionghong; Shang, Bin; Zhang, Min; You, Li; Hao, Chuan-Ming; Zhu, Tongying

    2017-01-01

    Background. Coronary artery calcification (CAC) contributes to high risk of cardiocerebrovascular diseases in dialysis patients. However, the risk factors for CAC initiation in peritoneal dialysis (PD) patients are not known clearly. Methods. Adult patients with baseline CaCS = 0 and who were followed up for at least 3 years or until the conversion from absent to any measurable CAC detected were included in this observational cohort study. Binary logistic regression was performed to identify the risk factors for CAC initiation in PD patients. Results. 70 patients recruited to our study were split into a noninitiation group (n = 37) and an initiation group (n = 33) according to the conversion of any measurable CAC during their follow-up or not. In univariate analysis, systolic blood pressure, serum phosphorus, fibrinogen, hs-CRP, serum creatinine, and triglycerides were positively associated with the initiation of CAC, while the high density lipoprotein and nPCR did the opposite function. Multivariate analysis revealed that hyperphosphatemia and hs-CRP were the independent risk factors for CAC initiation after adjustments. Conclusions. Hyperphosphatemia and hs-CRP were the independent risk factors for CAC initiation in PD patients. These results suggested potential clinical strategies to prevent the initiation of CAC in PD patients.

  13. The relationship of urotensin II with insulin resistance and hs-CRP in patients having PCOS.

    Science.gov (United States)

    Yilmaz, Özgür; Calan, Ozlem; Kume, Tuncay; Calan, Mehmet

    2013-11-01

    UrotensinII (UII), one of the most potent vasoconstrictor proteins, may be associated with insulin resistance. The objective of our research was to determine the level of UII in sera of patients with PCOS and to investigate the relationship between UII and insulin resistance in patients with PCOS. Fourty seven PCOS patients and 42 healthy women were included in the study. Serum fasting glucose, insulin, UII, free testosterone and hs-CRP levels of the patients were examined. The HOMA-IR formula was used to calculate insulin resistance. Insulin sensitivity was calculated by using the QUICKI-IS formula. The two groups did not show any a significant difference in terms of age and BMI (p > 0.05). Serum UII, hs-CRP, insulin levels and the HOMA-IR were significantly higher in the patients with PCOS (p hs-CRP(r = 0.51, p < 0.001). Our study data suggested that UII may have a role in the pathophysiology of insulin resistance and increased cardiovascular risk, which are commonly found in patients with PCOS.

  14. Rapid discrimination and feature extraction of three Chamaecyparis species by static-HS/GC-MS.

    Science.gov (United States)

    Chen, Ying-Ju; Lin, Chun-Ya; Cheng, Sen-Sung; Chang, Shang-Tzen

    2015-01-28

    This study aimed to develop a rapid and accurate analytical method for discriminating three Chamaecyparis species (C. formosensis, C. obtusa, and C. obtusa var. formosana) that could not be easily distinguished by volatile compounds. A total of 23 leaf samples from three species were analyzed by static-headspace (static-HS) coupled with gas chromatography-mass spectrometry (GC-MS). The static-HS procedure, whose experimental parameters were properly optimized, yielded a high Pearson correlation-based similarity between essential oil and VOC composition (r = 0.555-0.999). Thirty-six major constituents were identified; along with the results of cluster analysis (CA), a large variation in contents among the three different species was observed. Principal component analysis (PCA) methods illustrated graphically the relationships between characteristic components and tree species. It was clearly demonstrated that the static-HS-based procedure enhanced greatly the speed of precise analysis of chemical fingerprint in small sample amounts, thus providing a fast and reliable tool for the prediction of constituent characteristics in essential oil, and also offering good opportunities for studying the role of these feature compounds in chemotaxonomy or ecophysiology.

  15. Association of HS6ST3 gene polymorphisms with obesity and triglycerides: gene x gender interaction.

    Science.gov (United States)

    Wang, Ke-Sheng; Wang, Liang; Liu, Xuefeng; Zeng, Min

    2013-12-01

    The heparan sulfate 6-O-sulfotransferase 3 (HS6ST3) gene is involved in heparan sulphate and heparin metabolism, and has been reported to be associated with diabetic retinopathy in type 2 diabetes.We hypothesized that HS6ST3 gene polymorphisms might play an important role in obesity and related phenotypes (such as triglycerides). We examined genetic associations of 117 single-nucleotide polymorphisms (SNPs) within the HS6ST3 gene with obesity and triglycerides using two Caucasian samples: the Marshfield sample (1442 obesity cases and 2122 controls), and the Health aging and body composition (Health ABC) sample (305 cases and 1336 controls). Logistic regression analysis of obesity as a binary trait and linear regression analysis of triglycerides as a continuous trait, adjusted for age and sex, were performed using PLINK. Single marker analysis showed that six SNPs in the Marshfield sample and one SNP in the Health ABC sample were associated with obesity (P triglycerides in the Marshfield sample (P triglycerides in the Marshfield sample. These findings contribute new insights into the pathogenesis of obesity and triglycerides and demonstrate the importance of gender differences in the aetiology.

  16. Exploring Dust Impacts on Tropical Systems from the NASA HS-3 Field Campaign

    Science.gov (United States)

    Nowottnick, Ed; Colarco, Pete; da Silva, Arlindo; Barahona, Donifan; Hlavka, Dennis

    2015-01-01

    One of the overall scientific goals of the NASA Hurricane and Severe Storm Sentinel (HS-3) field campaign is to better understand the role of the Saharan Air Layer (SAL) in tropical storm development. During the 2012 HS-3 deployment, the Cloud Physics Lidar (CPL) observed dust within SAL air in close proximity to a developing Nadine (September 11, 2012). Throughout the mission, the NASA GEOS-5 modeling system supported HS-3 by providing 0.25 degrees resolution 5-day global forecasts of aerosols, which were used to support mission planning. The aerosol module was radiatively interactive within the GEOS-5 model, but aerosols were not directly coupled to cloud and precipitation processes. In this study we revisit the aerosol forecasts with an updated version of the GEOS-5 model. For the duration of Hurricane Nadine, we run multiday climate simulations leading up to each respective Global Hawk flight with and without aerosol direct interaction. For each set of simulations, we compare simulated dust mass fluxes to identify differences in SAL entrainment related to the interaction between dust aerosols and the atmosphere. We find that the direct effects of dust induce a low level anticyclonic circulation that temporarily shields Nadine from the intrusion of dry air, leading to a more intense storm.

  17. Inhibitory effects of HS014 on glutamate release in astrocytes chronically treated with morphine

    Institute of Scientific and Technical Information of China (English)

    Haichen Chu; Zejun Niu; Zhao Yang; Xuefeng Zhang

    2010-01-01

    Previous studies have confirmed that the number of glial fibrillary acidic protein-positive cells significantly increases during morphine tolerance.However,morphine tolerance is reversed with melanocortin receptor antagonists,and analgesic action is enhanced accordingly.However,these mechanisms remain unclear.In the present study,following addition of morphine to Wistar rat spinal cord astrocytes,glutamate levels in the supematant significantly increased(P<0.05).At 30-120minutes following addition of intervention agent to spinal cord astrocytes,naloxone significantly increased glutamate release in morphine-tolerant model cells(P < 0.05),while melanocortin receptor antagonist HS014 decreased glutamate release(P < 0.05).Additional naloxone and HS014 to astrocytes significantly decreased glutamate release compared with additional naloxone alone(P < 0.01).Results from the present study demonstrated that glutamate release was increased in spinal cord astrocytes co-cultured with morphine.Naloxone increased glutamate release,and HS014 reduced glutamate release.

  18. Relationship Between the Hypersensitive c-Reactive Protein (hs-CRP) Level and the Prognosis of Acute Brainstem Infarction.

    Science.gov (United States)

    Zhang, Xia; Huang, Wen-Juan; Yu, Zhi-Gang

    2015-05-01

    The objective of this study is to explore the relationship between the hypersensitive c-reactive protein (hs-CRP) level and the prognosis of acute brainstem infarction. Serum levels of hs-CRP were measured in 68 patients with acute brainstem infarction 72 h after disease onset. The hs-CRP levels in the U.S. National Institutes of Health Stroke Scale (NIHSS) score group and in the modified RANKIN scale (mRS) score group were compared. The independent risk factors of brainstem infarction were analyzed using Logistic binary regression. The hs-CRP level was significantly higher in the group with NIHSS >5 compared with the one with NIHSS ≤ 5 (P = 0.004). In the group with mRS > 2, the age, smoking history, and blood glucose level were significantly higher than those in the group with mRS ≤ 2 (P hs-CRP level was significantly higher (P = 0.001). Age and hs-CRP level were the independent prognostic factors of the brainstem infarction. The serum hs-CRP level is closely related with the severity and prognosis of brainstem infarction, and is an independent risk factor of acute brainstem infarction.

  19. High-normal levels of hs-CRP predict the development of non-alcoholic fatty liver in healthy men

    Science.gov (United States)

    Lee, Jieun; Yoon, Kijung; Ryu, Seungho; Chang, Yoosoo; Kim, Hyoung-Ryoul

    2017-01-01

    We performed a follow-up study to address whether high sensitivity C-reactive protein (hs-CRP) levels within the normal range can predict the development of non-alcoholic fatty liver disease (NAFLD) in healthy male subjects. Among15347 male workers between 30 and 59 years old who received annual health check-ups in 2002, a NAFLD-free cohort of 4,138 was followed through December 2009. Alcohol consumption was assessed with a questionnaire. At each visit, abdominal ultrasonography was performed to identify fatty liver disease. The COX proportional hazard model was used to evaluate the relationship between hs-CRP and incident NAFLD. During the follow-up period, 28.8% (1191 of 4138) of participants developed NAFLD. The hazard ratios of NAFLD were increased by hs-CRP categories within the normal range in the non-adjusted model and age-adjusted model. After adjusting for age, exercise, smoking, BMI, systolic BP, triglyceride, and fasting glucose, these incidences were only increased between the lowest and the highest hs-CRP categories. The risk for NAFLD increased as the hs-CRP level increased (p< 0.001). As the hs-CRP level increased within the healthy cohort, the risk of developing NAFLD increased. This trend remained true even if the hs-CRP level remained within the normal range. hs-CRP can be used as a predictor of NAFLD, as well as other obesity-associated diseases. Therefore, individuals with higher hs-CRP levels (even within the normal range) may require appropriate follow-up and management to prevent NAFLD development. PMID:28234943

  20. The Chicken β-Globin 5′HS4 Boundary Element Blocks Enhancer-Mediated Suppression of Silencing

    Science.gov (United States)

    Walters, Mark C.; Fiering, Steven; Bouhassira, Eric E.; Scalzo, David; Goeke, Scott; Magis, Wendy; Garrick, David; Whitelaw, Emma; Martin, David I. K.

    1999-01-01

    A constitutive DNase I-hypersensitive site 5′ of the chicken β-globin locus, termed 5′HS4 or cHS4, has been shown to insulate a promoter from the effect of an upstream enhancer and to reduce position effects on mini-white expression in Drosophila cells; on the basis of these findings, it has been designated a chromatin insulator. We have examined the effect of the cHS4 insulator in a system that assays both the level of gene expression and the rate of transcriptional silencing. Because transgenes flanked by insulator elements are shielded from position effects in Drosophila cells, we tested the ability of cHS4 to protect transgenes from position effects in mammalian cells. Flanking of an expression vector with the cHS4 insulator in a colony assay did not increase the number of G418-resistant colonies. Using lox/cre-based recombinase-mediated cassette exchange to control integration position, we studied the effect of cHS4 on the silencing of an integrated β-geo reporter at three genomic sites in K562 erythroleukemia cells. In this assay, enhancers act to suppress silencing but do not increase expression levels. While cHS4 blocked enhancement at each integration site, the strength of the effect varied from site to site. Furthermore, at some sites, cHS4 inhibited the enhancer effect either when placed between the enhancer and the promoter or when placed upstream of the enhancer. These results suggest that the activity of cHS4 is not dominant in all contexts and is unlikely to prevent silencing at all genomic integration sites. PMID:10207095

  1. The chicken beta-globin 5'HS4 boundary element blocks enhancer-mediated suppression of silencing.

    Science.gov (United States)

    Walters, M C; Fiering, S; Bouhassira, E E; Scalzo, D; Goeke, S; Magis, W; Garrick, D; Whitelaw, E; Martin, D I

    1999-05-01

    A constitutive DNase I-hypersensitive site 5' of the chicken beta-globin locus, termed 5'HS4 or cHS4, has been shown to insulate a promoter from the effect of an upstream enhancer and to reduce position effects on mini-white expression in Drosophila cells; on the basis of these findings, it has been designated a chromatin insulator. We have examined the effect of the cHS4 insulator in a system that assays both the level of gene expression and the rate of transcriptional silencing. Because transgenes flanked by insulator elements are shielded from position effects in Drosophila cells, we tested the ability of cHS4 to protect transgenes from position effects in mammalian cells. Flanking of an expression vector with the cHS4 insulator in a colony assay did not increase the number of G418-resistant colonies. Using lox/cre-based recombinase-mediated cassette exchange to control integration position, we studied the effect of cHS4 on the silencing of an integrated beta-geo reporter at three genomic sites in K562 erythroleukemia cells. In this assay, enhancers act to suppress silencing but do not increase expression levels. While cHS4 blocked enhancement at each integration site, the strength of the effect varied from site to site. Furthermore, at some sites, cHS4 inhibited the enhancer effect either when placed between the enhancer and the promoter or when placed upstream of the enhancer. These results suggest that the activity of cHS4 is not dominant in all contexts and is unlikely to prevent silencing at all genomic integration sites.

  2. Targeted deletion of 5'HS1 and 5'HS4 of the beta-globin locus control region reveals additive activity of the DNaseI hypersensitive sites.

    Science.gov (United States)

    Bender, M A; Roach, J N; Halow, J; Close, J; Alami, R; Bouhassira, E E; Groudine, M; Fiering, S N

    2001-10-01

    The mammalian beta-globin locus is a multigenic, developmentally regulated, tissue-specific locus from which gene expression is regulated by a distal regulatory region, the locus control region (LCR). The functional mechanism by which the beta-globin LCR stimulates transcription of the linked beta-like globin genes remains unknown. The LCR is composed of a series of 5 DNaseI hypersensitive sites (5'HSs) that form in the nucleus of erythroid precursors. These HSs are conserved among mammals, bind transcription factors that also bind to other parts of the locus, and compose the functional components of the LCR. To test the hypothesis that individual HSs have unique properties, homologous recombination was used to construct 5 lines of mice with individual deletions of each of the 5'HSs of the endogenous murine beta-globin LCR. Here it is reported that deletion of 5'HS1 reduces expression of the linked genes by up to 24%, while deletion of 5'HS4 leads to reductions of up to 27%. These deletions do not perturb the normal stage-specific expression of genes from this multigenic locus. In conjunction with previous studies of deletions of the other HSs and studies of deletion of the entire LCR, it is concluded that (1) none of the 5'HSs is essential for nearly normal expression; (2) none of the HSs is required for proper developmental expression; and (3) the HSs do not appear to synergize either structurally or functionally, but rather form independently and appear to contribute additively to the overall expression from the locus.

  3. High sensitivity C - reactive protein (hs-CRP) and clinical characteristics, endocrine, metabolic profile in Indian women with PCOS: a correlation

    OpenAIRE

    Sunita Jaiprakash Ramanand; Jaiprakash B Ramanand; Girish T. Raparti; Ravi R Ghanghas; Nimish R. Halasawadekar; Praveenkumar T. Patil; Mayur P. Pawar; Mayur P. Shinde

    2014-01-01

    Background: Role of hs-CRP was studied in PCOS women. Methods: Correlation between serum hs-CRP and endocrine, metabolic profile was studied in 30 healthy women and 88 PCOS women. In PCOS women correlation between hs-CRP and clinical characteristics viz obesity, infertility, acne, hirsutism, acanthosis nigricans (AN) was also studied. Serum levels of hs-CRP, Luteinizing hormone (LH), Follicle stimulating hormone (FSH), LH:FSH ratio, Testosterone (Testo), fasting insulin, fasting blood gluc...

  4. HS-AFP在肝癌早期诊断及鉴别诊断中的价值%The Value of HS-AFP in the Early Diagnosis and DifferentiaI Diagnosis of Hepatocellular Carcinoma

    Institute of Scientific and Technical Information of China (English)

    贲其稳; 倪润洲; 肖明兵; 陆翠华; 李立人; 毛振彪

    2008-01-01

    目的:探讨肝癌特异性甲胎蛋白(HS-AFP)对肝细胞癌的诊断及鉴别诊断价值.方法:对105例肝癌患者及151例良性肝病血清HS-AFP及AFP进行检测,并对10例HS-AFP阳性的良性肝病患者进行15个月随访观察.HS-AFP采用不连续缓冲系统PAGE结合Western blot分离检测,AFP采用化学发光法测定.结果:105例肝癌患者HS-AFP和AFP>200μg/L阳性率分别为60.0%和50.5%(P>0.05),良性肝痛患者HS-AFP阳性率显著低于AFP>200μg/L的阳性率(P<0.05).AFP处于50-400μg/L范围的肝癌组HS-AFP阳性率为77.1%.良性肝病组阳性率为13.6%(P<0.01).151例良性肝病中有10例HS-AFP出现阳性,其中3例于随访期间检出肝癌.11例小体积肝癌HS-AFP阳性率(45.5%)高于AFP>200μg/L的阳性率(18.2%).结论:HS-AFP对肝癌的诊断价值优于AFP浓度.对肝癌高危人群监测HS-AFP有助于肝癌的早期诊断.对血清AFP中低浓度升高的良恶性肝病有较高的鉴别诊断价值.

  5. NT-proBNP和hs-CRP在急性冠脉综合征诊断中的价值%The value of NT-proBNP and hs-CRP in acute coronary syndrome diagnosis

    Institute of Scientific and Technical Information of China (English)

    李茂

    2014-01-01

    Objective:To explore the levels of NT-proBNP and hs-CRP in serum of patients with acute coronary syndrome. Methods:50 cases of ACS were as the observation group;40 cases of healthy persons were as the control group.We detected the changes in the levels of NT-proBNP and hs-CRP in serum of the two groups.Results:In the observation group,the levels of NT-proBNP and hs-CRP in serum were significantly increased.Compared with the control group,the difference was statistically significant(P<0.05).Conclusion:The detection of NT-proBNP and hs-CRP is of great significance in the diagnosis of acute coronary syndrome.%目的:观察急性冠脉综合征(ACS)患者血清中NT-proBNP和hs-CRP水平。方法:50例ACS患者作观察组,40例健康者作对照组,检测两组血清中NT-proBNP和hs-CRP水平的变化。结果:观察组血清中NT-proBNP与hs-CRP水平均显著升高,与对照组相比差异有统计学意义(P<0.05)。结论:NT-proBNP和hs-CRP的检测对急性冠脉综合征的诊断具有重要意义。

  6. Increased hsCRP is associated with higher risk of aortic valve replacement in patients with aortic stenosis

    DEFF Research Database (Denmark)

    Blyme, Adam; Asferg, Camilla; Nielsen, O. W.

    2016-01-01

    Objective To investigate relations between inflammation and aortic valve stenosis (AS) by measuring high-sensitivity C-reactive protein, at baseline (hsCRP(0)) and after 1year (hsCRP(1)) and exploring associations with aortic valve replacement (AVR). Design We examined 1423 patients from...... the Simvastatin and Ezetimibe in Aortic Stenosis study. Results During first year of treatment, hsCRP was reduced both in patients later receiving AVR (2.3 [0.9-4.9] to 1.8 [0.8-5.4] mg/l, p...) predicted later AVR (HR=1.17, paortic valve area (AVA) and other risk factors. A higher rate of AVR was observed in the group with high hsCRP(0) and an increase during the first year (AVR(highCRP0CRP1inc)=47.3% versus AVR(highCRP0CRP1dec)=27.5%, p

  7. Reactions of superoxide dismutases with HS(-)/H2S and superoxide radical anion: An in vitro EPR study.

    Science.gov (United States)

    Bolić, Bojana; Mijušković, Ana; Popović-Bijelić, Ana; Nikolić-Kokić, Aleksandra; Spasić, Snežana; Blagojević, Duško; Spasić, Mihajlo B; Spasojević, Ivan

    2015-12-01

    Interactions of hydrogen sulfide (HS(-)/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS(-)/H2S and superoxide radical anion O2.- on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS(-)/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu(2+) was reduced to Cu(1+), while manganese appears to be released from MnSOD active center. Untreated and O2.- treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe(3+) and Mn(2+). Our study provides new details on the mechanisms of (patho)physiological effects of HS(-)/H2S.

  8. Role of inflammation as reflected by serum hsCRP and ferritin level in major depressive disorder

    Directory of Open Access Journals (Sweden)

    Soma Gupta

    2016-09-01

    Conclusions: The significant change in hsCRP and Ferritin which is not associated with grade wise alteration suggests that probably inflammation is the early event which causes depression. Therefore no gradual change is observed with increase in severity of the disease. hsCRP can be used as a biomarker of Inflammation in MDD cases. [Int J Res Med Sci 2016; 4(9.000: 4072-4077

  9. High-normal levels of hs-CRP predict the development of non-alcoholic fatty liver in healthy men.

    Science.gov (United States)

    Lee, Jieun; Yoon, Kijung; Ryu, Seungho; Chang, Yoosoo; Kim, Hyoung-Ryoul

    2017-01-01

    We performed a follow-up study to address whether high sensitivity C-reactive protein (hs-CRP) levels within the normal range can predict the development of non-alcoholic fatty liver disease (NAFLD) in healthy male subjects. Among15347 male workers between 30 and 59 years old who received annual health check-ups in 2002, a NAFLD-free cohort of 4,138 was followed through December 2009. Alcohol consumption was assessed with a questionnaire. At each visit, abdominal ultrasonography was performed to identify fatty liver disease. The COX proportional hazard model was used to evaluate the relationship between hs-CRP and incident NAFLD. During the follow-up period, 28.8% (1191 of 4138) of participants developed NAFLD. The hazard ratios of NAFLD were increased by hs-CRP categories within the normal range in the non-adjusted model and age-adjusted model. After adjusting for age, exercise, smoking, BMI, systolic BP, triglyceride, and fasting glucose, these incidences were only increased between the lowest and the highest hs-CRP categories. The risk for NAFLD increased as the hs-CRP level increased (phs-CRP level increased within the healthy cohort, the risk of developing NAFLD increased. This trend remained true even if the hs-CRP level remained within the normal range. hs-CRP can be used as a predictor of NAFLD, as well as other obesity-associated diseases. Therefore, individuals with higher hs-CRP levels (even within the normal range) may require appropriate follow-up and management to prevent NAFLD development.

  10. High sensitive C-reactive protein (hs-CRP level and biochemical parameters for prehypertension and prediabetes diagnosis

    Directory of Open Access Journals (Sweden)

    Yuttana Sudjaroen

    2015-01-01

    Full Text Available Context: Coronary atherosclerosis still presents one of the main causes of death. Efficacious prevention should focus on the early control of cardiovascular risk factors, including lipid profiles, which are unable early detect in subclinical cases. High-sensitive C-reactive protein (hs-CRP can prove to be an early cardiac risk predictor. Aims: 1 To compare hs-CRP levels between healthy volunteer with normal blood pressure and those with prehypertension, and 2 to use hs-CRP levels along with other risks to be a cardiac risk predictor. Setting and Design: This was a cross-sectional study for 6 months′ duration from January to June 2013 at Kudjab Hospital located in Udon Thani province, Thailand. Materials and Methods: Forty (40 healthy volunteers with prehypertension and 40 volunteers with normal blood pressure were included in the study. Both groups were similar in age range and sex. Twelve-hour (12-h fasting blood samples were collected from all the participants. Serum was assayed for hs-CRP and lipid profile. Results: All of parameters were statistically significant difference (P < 000.1. The hs-CRP level (6.27 ± 7.8 mg/L was elevated in the prehypertension group. The relative risk of hs-CRP for prehypertension was 6.3 with the odds ratio of 15.48, whereas the relative risk of lipid profiles for prehypertension prediction was only 1.28, with the odds ratio of 1.67. Statistical Analysis: SPSS version 11.0 using the unpaired t-test for comparing demographic data and blood parameters and risk prediction of hs-CRP and lipid profiles were calculated by relative risk with odds ratio [95% confidence interval (CI]. Conclusions: Hs-CRP is an early cardiac risk predictor even with normal lipid profile, and can help measure additional risk especially subclinical people such as prehypertension.

  11. Determination of aromatic compounds in eluates of pyrolysis solid residues using HS-GC-MS and DLLME-GC-MS.

    Science.gov (United States)

    Bernardo, Maria S; Gonçalves, M; Lapa, N; Barbosa, R; Mendes, B; Pinto, F; Gulyurtlu, Ibrahim

    2009-11-15

    A method for the determination of 15 aromatic hydrocarbons in eluates from solid residues produced during the co-pyrolysis of plastics and pine biomass was developed. In a first step, several sampling techniques (headspace solid phase microextraction (HS-SPME), static headspace sampling (HS), and dispersive liquid-liquid microextraction (DLLME) were compared in order to evaluate their sensitivity towards these analytes. HS-SPME and HS sampling had the better performance, but DLLME was itself as a technique able to extract volatiles with a significant enrichment factor. HS sampling coupled with GC-MS was chosen for method validation for the analytes tested. Calibration curves were constructed for each analyte with correlation coefficients higher than 0.999. The limits of detection were in the range of 0.66-37.85 ng/L. The precision of the HS method was evaluated and good repeatability was achieved with relative standard deviations of 4.8-13.2%. The recoveries of the analytes were evaluated by analysing fortified real eluate samples and were in the range of 60.6-113.9%. The validated method was applied in real eluate samples. Benzene, toluene, ethylbenzene and xylenes (BTEX) were the compounds in higher concentrations. The DLLME technique coupled with GC-MS was used to investigate the presence of less volatile contaminants in eluate samples. This analysis revealed the presence of significant amounts of alkyl phenols and other aromatic compounds with appreciable water solubility.

  12. The role of hs-CRP, D-dimer and fibrinogen in differentiating etiological subtypes of ischemic stroke.

    Science.gov (United States)

    Liu, Li-Bin; Li, Mu; Zhuo, Wen-Yan; Zhang, Yu-Sheng; Xu, An-Ding

    2015-01-01

    The aim of this study was to evaluate the diagnostic value of the serum biochemical markers high-sensitivity C-reactive protein (hs-CRP), D-dimer (DD) and fibrinogen (Fg) in differentiating etiological subtypes of ischemic stroke. This study was a retrospective case-only study, consecutively including patients with acute ischemic stroke. All patients were classified into subtypes using the TOAST classification system. A total of 317 patients were evaluated. Hs-CRP and DD levels were significantly different among the subtypes and were the highest in CE, followed by LAA and SAA; no significant difference between the subtypes was found for Fg. Hs-CRP > 6.96 mg/L was classified as the CE subtype, with a sensitivity of 41% and a specificity of 74%; DD > 791.30 ng/mL was classified as CE, with a sensitivity of 58% and a specificity of 78%. The combination of hs-CRP and DD classification as CE yielded a sensitivity of 65% and a specificity of 91%. DD > 791.30 ng/mL was considered an independent predictive factor of CE. Hs-CRP and DD could be useful for identifying the etiological subtypes of acute ischemic stroke, especially for predicting CE. The diagnostic value of DD was higher than that of hs-CRP.

  13. Obesity indices and metabolic markers are related to hs-CRP and adiponectin levels in overweight and obese females.

    Science.gov (United States)

    Sanip, Zulkefli; Ariffin, Farah Diana; Al-Tahami, Belqes Abdullah Mohammed; Sulaiman, Wan Azman Wan; Rasool, Aida Hanum Ghulam

    2013-01-01

    Obese subjects had increased serum high sensitivity C-reactive protein (hs-CRP), decreased adiponectin levels, and impaired microvascular endothelial function compared to lean subjects. We investigated the relationships of serum hs-CRP, adiponectin and microvascular endothelial function with obesity indices and metabolic markers in overweight and obese female subjects. Anthropometric profile, body fat composition, biochemical analysis, serum hs-CRP and adiponectin levels, and microvascular endothelial function were measured in 91 female subjects. Microvascular endothelial function was determined using laser Doppler fluximetry and the process of iontophoresis. Mean age and body mass index (BMI) of subjects were 34.88 (7.87) years and 32.93 (4.82) kg/m(2). hs-CRP levels were positively correlated with weight, BMI, waist circumference, hip circumference, body fat and visceral fat. Adiponectin levels were positively correlated with insulin sensitivity index (HOMA-%S), and inversely correlated with waist hip ratio, triglyceride, fasting insulin and insulin resistance index (HOMA-IR). No relationship was seen between microvascular endothelial function and obesity indices, and metabolic markers. In overweight and obese female subjects, hs-CRP levels were correlated with obesity indices while adiponectin levels were inversely correlated with obesity indices and metabolic markers. No significant relationship was seen between microvascular endothelial function with obesity indices and metabolic markers including hs-CRP and adiponectin in female overweight and obese subjects.

  14. Elevated copper, hs C-reactive protein and dyslipidemia in drug free schizophrenia: Relation with psychopathology score.

    Science.gov (United States)

    Devanarayanan, Sivasankar; Nandeesha, Hanumanthappa; Kattimani, Shivanand; Sarkar, Siddharth; Jose, Jancy

    2016-12-01

    Inflammation, dyslipidemia and altered copper levels have been reported in several psychiatric disorders, including schizophrenia. However, their association with the severity of psychopathology in schizophrenia is yet to be established. The present study was designed to assess the serum levels of copper, highly sensitive C-reactive protein (hs-CRP) and lipid profile and to explore their association with psychopathology scores in schizophrenia. 40 cases and 40 controls were included in the study. Serum copper, hs-CRP and lipid profile were estimated in all the subjects. Disease severity was assessed using Positive and Negative Syndrome Scale (PANSS). Copper, hs-CRP, total cholesterol and LDL-Cholesterol were significantly increased and HDL-Cholesterol was significantly reduced in schizophrenia cases when compared with controls. Copper was positively correlated with hs-CRP (r=0.338, p=0.003). Total cholesterol was significantly correlated with PANSS total (r=0.452, p=0.003) and negative symptom scores (r=0.337, p=0.033). Triacylglycerol was positively correlated with general psychopathology symptom score (r=0.416, p=0.008). Copper and hs-CRP were increased and correlated well with each other in schizophrenia cases. Though total cholesterol and triacylglycerol showed positive association with severity of the psychopathology, copper and hs-CRP were not associated with the disease severity. Copyright © 2016 Elsevier B.V. All rights reserved.

  15. Association of HS6ST3 gene polymorphisms with obesity and triglycerides: gene × gender interaction

    Indian Academy of Sciences (India)

    Ke-Sheng Wang; Liang Wang; Xuefeng Liu; Min Zeng

    2013-12-01

    The heparan sulfate 6-O-sulfotransferase 3 (HS6ST3) gene is involved in heparan sulphate and heparin metabolism, and has been reported to be associated with diabetic retinopathy in type 2 diabetes. We hypothesized that HS6ST3 gene polymorphisms might play an important role in obesity and related phenotypes (such as triglycerides). We examined genetic associations of 117 single-nucleotide polymorphisms (SNPs) within the HS6ST3 gene with obesity and triglycerides using two Caucasian samples: the Marshfield sample (1442 obesity cases and 2122 controls), and the Health aging and body composition (Health ABC) sample (305 cases and 1336 controls). Logistic regression analysis of obesity as a binary trait and linear regression analysis of triglycerides as a continuous trait, adjusted for age and sex, were performed using PLINK. Single marker analysis showed that six SNPs in the Marshfield sample and one SNP in the Health ABC sample were associated with obesity $(P \\lt 0.05)$. SNP rs535812 revealed a stronger association with obesity in meta-analysis of these two samples $(P = 0.0105)$. The T–A haplotype from rs878950 and rs9525149 revealed significant association with obesity in the Marshfield sample $(P = 0.012)$. Moreover, nine SNPs showed associations with triglycerides in the Marshfield sample $(P \\lt 0.05)$ and the best signal was rs1927796 $(P = 0.00858)$. In addition, rs7331762 showed a strong gene × gender interaction $(P = 0.00956)$ for obesity while rs1927796 showed a strong gene × gender interaction $(P = 0.000625)$ for triglycerides in the Marshfield sample. These findings contribute new insights into the pathogenesis of obesity and triglycerides and demonstrate the importance of gender differences in the aetiology.

  16. 佳能发布伊克萨斯IXUS 1000 HS

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    佳能IXU S1000 HS具有10倍光学变焦.但机身厚度仅约22.3毫米.在秉承伊克萨斯系列时尚设计风格的同时.实现了高倍光学变焦.IXU S1000 HS共有3种机身色彩可供选择,包括玛瑙褐,宝石粉和珍珠银.不但配置了“HS SYSTEM”.

  17. Tribological resistance of high speed steel HS 6-5-2 remelted with electric arc

    Directory of Open Access Journals (Sweden)

    A. Dziedzic

    2009-07-01

    Full Text Available The intensity of tribological wear of the high speed steel HS 6-5-2 remelted with the GTAW method has been compared to the heat treatment steel in a conventional way. Moreover, the types of the wear appeared during the friction. The tribiological research, were done in the technically dry friction conditions on a testing machine of the pin-on-disc T-01M. The smallest intensity of wear was shown by the high speed steel remelted with the parameters leading to obtain the biggest speed of cooling of the molten metal. The main wear type appearing during the research, was the abrasion and adhesive wear.

  18. Material characteristics of CLEARCERAM-Z HS for use in large diameter mirror blanks

    Science.gov (United States)

    Kishi, Takayuki; Goto, Naoyuki; Hoffman, Brion; Kawashima, Yasuyuki; Suzuki, Kenji; Yoshizawa, Kazuharu; Suzuki, Kaito

    2010-07-01

    There is growing interest within the Astronomical community in the development and use of very large aperture telescopes which will incorporate the latest advancements in optical materials. Two of the most notable of these large size telescope projects, the Thirty Meter Telescope (TMT) and European Extremely Large Telescope (E-ELT), will use mirror segments in the actively controlled primary mirrors. In this paper we present the results of a material characteristics study on Ohara CLEARCERAM®-Z HS large diameter blanks which includes data on the CTE, CTE uniformity, residual stress and internal quality targeting potential use in the TMT Primary Mirror Segment Banks.

  19. Study on the changes of RDW and hs-CRP in patients with preeclampsia%子痫前期患者 RDW 与 hs-CRP 检测的临床价值

    Institute of Scientific and Technical Information of China (English)

    王爱兰; 刘庆翠

    2015-01-01

    Objective:To evaluate the relationship of RDW and hs-CRP with the presence and severity of preeclampsia .Methods :RDW was meas-ured using blood cell counter ,and hs-CRP level of patients with preeclampsia was measured through ELISA method .Results:The RDW ,systolic and diastolic blood pressure ,proteinuria ,white blood cell and hs-CRP levels were significantly higher in preeclampsia group .Subgroup analysis revealed that RDW levels were significantly increased in patients with severe preeclampsia when compared to the patients with mild preeclampsia .Conclusion:The RDW and hs-CRP levels were significantly higher in preeclampsia group than control pregnancy patients ,and they increased with the increase of severity of disease ,the was a positive correlation between them .RDW can be used for indicator of severity of disease .%目的:评价红细胞分布宽度(RDW)及超敏C反应蛋白(hs -CRP)与子痫前期发病及严重程度的关系.方法:采用血细胞计数器测量 RDW值 ,酶联免疫吸附法(ELISA)测定子痫前期患者血清中CRP水平.结果:子痫前期组RDW、收缩压和舒张压、尿蛋白、白细胞和hs -CRP显著增高.亚组分析表明 ,重度子痫前期患者RDW水平较轻度组显著升高.结论:子痫前期患者RDW和hs-CRP明显高于正常妊娠者 ,且随着疾病严重程度的增加而升高 ,两者呈正相关.RDW可作为判断疾病轻重的指标.

  20. 功能化CdS∶Mn荧光探针测定hs-DNA%Detection of hs-DNA by functinal CdS∶Mn fluorescent probe

    Institute of Scientific and Technical Information of China (English)

    张艺; 李紫薇; 何晓燕; 王兴磊

    2015-01-01

    在油胺溶剂中,利用溶剂热法于160℃加热反应12 h,合成了CdS∶Mn量子点,并用巯基乙酸对合成的量子点进行了包裹.借助X射线衍射分析、傅里叶红外光谱、荧光谱图等测试方法对材料进行表征,数据表明,合成的CdS∶Mn量子点JCPDS#为80-0019,在CdS∶Mn量子点上成功修饰上了羧基(-COOH),CdS∶Mn晶体修饰前后主要发射峰从526 nm蓝移至488 nm.以COOH-CdS∶Mn功能化材料为荧光探针,基于DNA对材料的荧光猝灭,实现了对DNA的定量测定.实验在pH=6.6条件下,功能化荧光材料的荧光强度和鲱鱼精DNA(hs-DNA)的质量浓度呈线性关系,线性方程为△F =78.69-0.179c,检出限为0.053 mg/L,相关系数R=0.998 8,RSD =0.27%.

  1. Effects of fluorinated and hydrogenated surfactants on human serum albumin at different pHs.

    Science.gov (United States)

    Sabín, Juan; Prieto, Gerardo; González-Pérez, Alfredo; Ruso, Juan M; Sarmiento, Félix

    2006-01-01

    Complexation between human serum albumin (HSA) and two different surfactants, one fully fluorinated (sodium perfluorooctanoate, SPFO) and one fully hydrogenated (sodium caprylate, SO), was studied using zeta-potential measurements and difference spectroscopy. The study was carried out at three different pHs, 3.2, 6.7, and 10.0. The spectroscopy study was performed at pHs 6.7 and 10.0, given that at pH 3.2 high turbidity was observed in the wide range of surfactant concentrations. The results were interpreted in terms of the electrostatic and hydrophobic contributions to the stability of the different phases formed in the water-surfactant-HSA system. Solutions and precipitates were observed in the concentration range investigated in more detail. Using Pace methods, the thermodynamic values of the surfactant-induced conformational changes in HSA were determined for sodium perfluorooctanoate in the concentration range 2-12 mmol dm(-3) at pH 6.7 and 5-22 mmol dm(-3) at pH 10.0. Electrophoretic measurements were used to characterize surfactant adsorption by determining the number of molecules adsorbed on the surface of HSA and the Gibbs energy of adsorption. Finally, the interactions between human serum albumin and other anionic surfactants studied by other authors were compared with those observed in the present work.

  2. Repeatability Using Automatic Tracing with Canon OCT- HS100 and Zeiss Cirrus HD-OCT 5000.

    Directory of Open Access Journals (Sweden)

    Rune Brautaset

    Full Text Available Optical coherence tomography (OCT, can be used in clinical practice to provide high resolution cross-sectional images of the retina, optic disc and macula structure. These measurements can be useful for early detection, diagnosis, monitoring and treatment guidance for retinal diseases. Therefore, repeatability of measurements in OCT is of great importance.Macula and optic disc parameters from the right eye of 30 healthy subjects were obtained twice with the Canon OCT-HS100 and Zeiss Cirrus HD-OCT 5000. Repeatability was evaluated by use of the coefficient of repeatability (CR and the coefficient of repeatability as a percentage of the mean (CR%, and the obtained measurements were compared between the instruments.CR% of optic disc parameters ranged between 0.90 and 22.22% and 0.00 and 16.00% with the Canon and Zeiss OCT respectively. For macular parameters CR% ranged between 0.62 and 2.81% and 0.99 and 1.81% with the Canon and Zeiss OCT respectively. No statistical difference could be found when comparing the CR of all macular and disc measurements between the instruments. Compared to our previously published data repeatability has significantly improved with the inclusion of automatic tracking systems with both the Canon and Zeiss OCT.Automatic tracking function improves repeatability in both Canon OCT-HS100 and Zeiss Cirrus HD-OCT 5000. However, measurements generated by the two instruments are still not interchangeable.

  3. Comparison of continuous and discontinuous counterimmunoelectrophoresis with immunodiffusion in identification of candida antibody using HS antigen.

    Science.gov (United States)

    Marier, R; Andriole, V T

    1978-07-01

    We have compared continuous counterimmunoelectrophoresis (CCIE) and discontinuous counterimmunoelectrophoresis (DCIE) with immunodiffusion (ID) in the identification of Candida albicans antibody using HS antigen. Eighteen sera with a titer of 1/1 to 1/32 1/32 by CCIE were titered by CCIE and ID. Fewer were positive by CCIE and ID (13 of 18 and 9 of 18, respectively). Furthermore, the titers were lower when measured by these two methods in comparison to DCIE (4 of 18 and 3 of 18 sera had titers greather than or equal to 1/8 in comparison with 7 of 18 greater than or equal to 1/8 by DCIE). Finally, DCIE was found to be much faster than CCIE and ID. After 20 min of electrophoresis, 17 of 21 sera were positive by DCIE, whereas none were positive by CCIE. Even after 90 min, only 10 of 21 were positive by CCIE, whereas 4 of 21 were positive by ID. The increased sensitivity and speed of DCIE over CCIE and ID suggest that DCIE is preferable to these other methods in the measurement of candida antibody using HS antigen.

  4. Hidradenitis supurativa in unusual sites

    Directory of Open Access Journals (Sweden)

    Gharami R

    2003-03-01

    Full Text Available A 35 year old man presented with multiple indurated plaques, studded with pus discharging sinuses and scarring on both legs for last 15 years. He was also having lichen amyloidosis on both forearms, Pus culture yielded growth of Staphylococcus aureus and tissue culture excluded deep fungal infection and mycobacterium tuberculosis bacilli.

  5. Hidradenitis supurativa in unusual sites

    Directory of Open Access Journals (Sweden)

    Gharami R

    2003-01-01

    Full Text Available A 35 year old man presented with multiple indurated plaques, studded with pus discharging sinuses and scarring on both legs for last 15 years. He was also having lichen amyloidosis on both forearms, Pus culture yielded growth of Staphylococcus aureus and tissue culture excluded deep fungal infection and mycobacterium tuberculosis bacilli.

  6. 联合检测Hcy、Cysc及hs-CRP对妊娠期高血压疾病的意义%The significance of joint detection of Hcy, CysC and hs-CRP for the diagnosis of HDP

    Institute of Scientific and Technical Information of China (English)

    王挺; 连炬飞; 周永贤; 郭莉; 陈汉彪

    2014-01-01

    Objective To evaluate the clinical value of serum homocysteine (Hcy),cystalin C (CysC) and high-sensitivity C-reactive protein (hs-CRP) joint detect for the diagnosis of hypertensive disorders complicating pregnancy (HDP).Method Serum levels of Hcy,CysC and hs-CRP of 86 HDP patients (HDP group) and 96 normal pregnant women (control group) were measured,analyzed and compared.Results Comparing HDP group and control group,severe HDP group and mild HDP group,there were significances differences in serum levels of Hcy,CysC and hs-CRP(P<0.05).Significant positive correlation exist between Hcy and Cysc,hs-CRP in HDP group (r=0.592,0.668),and the serum levels of Hcy,CysC and hs-CRP is positively correlated with the severity of HDP(r=0.759,0.266,0.365).Conclusion The serum levels of Hcy,CysC and hs-CRP in HDP patient is positively correlated with the severity of HDP.Joint detection of the serum levels of Hcy,CysC and hs-CRP is important for the diagnosis of HDP and illness progression and prognosis.%目的 探讨血清同型半胱氨酸(Hcy)、胱抑素C(CysC)及超敏-C反应蛋白(hs-CRP)联合检测对诊断妊娠期高血压疾病(HDP)的临床价值.方法 选取86例妊娠期高血压疾病患者(HDP组)和96例正常妊娠孕妇(对照组),分别检测血清Hcy 、CysC和hs-CRP,并对结果进行分析比较.结果 HDP组与对照组比较,重度HDP组与轻度HDP组比较,Hcy、CysC和hs-CRP差异均有统计学意义(P<0.05).HDP组Hcy与CysC、hs-CRP呈显著正相关(r=0.592、0.668),且Hcy 、CysC和hs-CRP浓度与HDP病情轻重呈正相关(r=0.759、0.266、0.365).结论 HDP患者血清Hcy 、Cysc和hs-CRP浓度与HDP病情轻重呈正相关,联合检测对诊断妊娠期高血压疾病及其病情进展、预后判断具有重要的指导意义.

  7. hs-CRP与2型糖尿病伴有高血压的关系%High plasmid hs-CRP level in patients with DM2 and HBP Daqing Oilfield General Hospital Laboratory Medicine

    Institute of Scientific and Technical Information of China (English)

    张丽娜; 车璐; 曹艳菲; 李新娜; 崔颖; 寇筱囡; 陈刚

    2013-01-01

    Objective We study if plasma levels of hs-CRP related to DM2+HBP patients. Methods hs-CRP were measured in subjects with DM2 and /or HBP, including 13 patients with DM2, 17 patients with HBP, 34 patients with DM2+HBP, and 25 healthy test control subjects. hs-CRP tested by Electro-Chemiluminescence Immuno Assay (ECLI). Results The plasma hs-CRP levels were significantly lower in the controls than in the DM2+HBP group (p<0.05), DM2 associated with HBP was also correlated with increased plasma hs-CRP levels (n=89, r =0.25, p=0.0160). Conclusions This study suggests that patients with two associated diseases have a more active inflammatory state.%目的:研究炎性反应标志物超敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP)与2型糖尿病(type 2 diabetes mel itus, DM2)伴随或者不伴随高血压是否存在相关性。方法入组13例2型糖尿病患者无合并症,17例高血压患者(high blood pressure, HBP),34例2型糖尿病伴随高血压患者(DM2+HBP),25例健康体检对照组,电化学免疫发光法测定血清hs-CRP。结果健康对照组hs-CRP水平明显低于HBP+DM2患者(p<0.05),DM2伴随HBP与hs-CRP水平显著相关(n=89, r=0.25, p=0.0160)。讨论 DM2+HBP患者具有相对活跃的炎性状态。

  8. Comparative genome analysis of cortactin and HS1: the significance of the F-actin binding repeat domain

    Directory of Open Access Journals (Sweden)

    Seggelen Vera

    2005-02-01

    Full Text Available Abstract Background In human carcinomas, overexpression of cortactin correlates with poor prognosis. Cortactin is an F-actin-binding protein involved in cytoskeletal rearrangements and cell migration by promoting actin-related protein (Arp2/3 mediated actin polymerization. It shares a high amino acid sequence and structural similarity to hematopoietic lineage cell-specific protein 1 (HS1 although their functions differ considerable. In this manuscript we describe the genomic organization of these two genes in a variety of species by a combination of cloning and database searches. Based on our analysis, we predict the genesis of the actin-binding repeat domain during evolution. Results Cortactin homologues exist in sponges, worms, shrimps, insects, urochordates, fishes, amphibians, birds and mammalians, whereas HS1 exists in vertebrates only, suggesting that both genes have been derived from an ancestor cortactin gene by duplication. In agreement with this, comparative genome analysis revealed very similar exon-intron structures and sequence homologies, especially over the regions that encode the characteristic highly conserved F-actin-binding repeat domain. Cortactin splice variants affecting this F-actin-binding domain were identified not only in mammalians, but also in amphibians, fishes and birds. In mammalians, cortactin is ubiquitously expressed except in hematopoietic cells, whereas HS1 is mainly expressed in hematopoietic cells. In accordance with their distinct tissue specificity, the putative promoter region of cortactin is different from HS1. Conclusions Comparative analysis of the genomic organization and amino acid sequences of cortactin and HS1 provides inside into their origin and evolution. Our analysis shows that both genes originated from a gene duplication event and subsequently HS1 lost two repeats, whereas cortactin gained one repeat. Our analysis genetically underscores the significance of the F-actin binding domain in

  9. h-FABP、hs-CRP、cTnT对急性心肌梗死诊断临床价值分析%Clinical Value of h - FABP, hs - CRP, cTnT examination to diagnose acute myocardial infarction

    Institute of Scientific and Technical Information of China (English)

    张翠玲; 姜艳梅; 高霞; 王欣

    2008-01-01

    [目的]通过对h-FABP、hs-CRP、cTnT 3项指标的联合检测为急性心肌梗死(AMI)的诊断提供可靠依据.[方法]cTnT检测采用金标记免疫渗滤法;hs-CRP检测采用免疫散射比浊法;h-FABP检测采用96T ELISA方法.[结果]健康组与AMI组比较,h-FABP:(5.46±0.19),(23.27±1.77)ng/mL,P<0.01;cTnT:(0.10±0.01),(0.64±0.11)ng/mL,P<0.01;hs-CRP:(0.85±0.10),(17.39±4.69)mg/L,P<0.01;cTnT、hs-CRP、h-FABP在40例AMI患者发作0~3 h阳性率分别为50%、55.26%、85%.3项指标联合分析阳性率可达98%.[结论]h-FABP、hs-CRP与cTnT联合检测对AMI早期诊断具有高灵敏性和特异性.

  10. Precedent fluctuation of serum hs-CRP to albumin ratios and mortality risk of clinically stable hemodialysis patients.

    Directory of Open Access Journals (Sweden)

    Jyh-Chang Hwang

    Full Text Available A high sensitivity C-reactive protein to albumin ratio (hs-CRP/Alb predicts mortality risk in patients with acute kidney injury. However, it varies dynamically. This study was conducted to evaluate whether a variation of this marker was associated with long-term outcome in clinically stable hemodialysis (HD patients.hs-CRP/Alb was checked bimonthly in 284 clinically stable HD outpatients throughout all of 2008. Based on the "slope" of trend equation derived from 5-6 hs-CRP/alb ratios for each patient, the total number of patients was divided into quartiles--Group 1: β≦ -0.13, n = 71; group 2: β>-0.13≦0.003; n = 71, group 3: β>0.003≦0.20; and group 4: β>0.20, n = 71. The observation period was from January 1, 2009 to August 31, 2012.Group 1+4 showed a worse long-term survival (p = 0.04 and a longer 5-year hospitalization stay than Group 2+3 (38.7±44.4 vs. 16.7±22.4 days, p<0.001. Group 1+4 were associated with older age (OR = 1.03, 95% CI = 1.01-1.05 and a high prevalence of congestive heart failure (OR = 2.02, 95% CI = 1.00-4.11. Standard deviation (SD of hs-CRP/Alb was associated with male sex (β = 0.17, p = 0.003, higher Davies co-morbidity score (β = 0.16, p = 0.03, and baseline hs-CRP (β = 0.39, p<0.001. Patients with lower baseline and stable trend of hs-CRP/Alb had a better prognosis. By multivariate Cox proportional methods, SD of hs-CRP/alb (HR: 1.05, 95% CI: 1.01-1.08 rather than baseline hs-CRP/Alb was an independent predictive factor for long-term mortality after adjusting for sex and HD vintage.Clinically stable HD patients with a fluctuating variation of hs-CRP/Alb are characterized by old age, and more co-morbidity, and they tend to have longer subsequent hospitalization stay and higher mortality risk.

  11. A Site-Specific Integrative Plasmid Found in Pseudomonas aeruginosa Clinical Isolate HS87 along with A Plasmid Carrying an Aminoglycoside-Resistant Gene.

    Directory of Open Access Journals (Sweden)

    Dexi Bi

    Full Text Available Plasmids play critical roles in bacterial fitness and evolution of Pseudomonas aeruginosa. Here two plasmids found in a drug-resistant P. aeruginosa clinical isolate HS87 were completely sequenced. The pHS87b plasmid (11.2 kb carries phage-related genes and function-unknown genes. Notably, pHS87b encodes an integrase and has an adjacent tRNAThr-associated attachment site. A corresponding integrated form of pHS87b at the tRNAThr locus was identified on the chromosome of P. aeruginosa, showing that pHS87b is able to site-specifically integrate into the 3'-end of the tRNAThr gene. The pHS87a plasmid (26.8 kb displays a plastic structure containing a putative replication module, stability factors and a variable region. The RepA of pHS87a shows significant similarity to the replication proteins of pPT23A-family plasmids. pHS87a carries a transposon Tn6049, a truncated insertion sequence ΔIS1071 and a Tn402-like class 1 integron which contains an aacA4 cassette that may confer aminoglycoside resistance. Thus, pHS87b is a site-specific integrative plasmid whereas pHS87a is a plastic antibiotic resistance plasmid. The two native plasmids may promote the fitness and evolution of P. aeruginosa.

  12. NaHS Protects Cochlear Hair Cells from Gentamicin-Induced Ototoxicity by Inhibiting the Mitochondrial Apoptosis Pathway.

    Directory of Open Access Journals (Sweden)

    Yaodong Dong

    Full Text Available Aminoglycoside antibiotics such as gentamicin could cause ototoxicity in mammalians, by inducing oxidative stress and apoptosis in sensory hair cells of the cochlea. Sodium hydrosulfide (NaHS is reported to alleviate oxidative stress and apoptosis, but its role in protecting aminoglycoside-induced hearing loss is unclear. In this study, we investigated the anti-oxidant and anti-apoptosis effect of NaHS in in vitro cultured House Ear Institute-Organ of Corti 1 (HEI-OC1 cells and isolated mouse cochlea. Results from cultured HEI-OC1 cells and cochlea consistently indicated that NaHS exhibited protective effects from gentamicin-induced ototoxicity, evident by maintained cell viability, hair cell number and cochlear morphology, reduced reactive oxygen species production and mitochondrial depolarization, as well as apoptosis activation of the intrinsic pathway. Moreover, in the isolated cochlear culture, NaHS was also demonstrated to protect the explant from gentamicin-induced mechanotransduction loss. Our study using multiple in vitro models revealed for the first time, the potential of NaHS as a therapeutic agent in protecting against aminoglycoside-induced hearing loss.

  13. NaHS Protects Cochlear Hair Cells from Gentamicin-Induced Ototoxicity by Inhibiting the Mitochondrial Apoptosis Pathway

    Science.gov (United States)

    Dong, Yaodong; Liu, Dongliang; Hu, Yue; Ma, Xiulan

    2015-01-01

    Aminoglycoside antibiotics such as gentamicin could cause ototoxicity in mammalians, by inducing oxidative stress and apoptosis in sensory hair cells of the cochlea. Sodium hydrosulfide (NaHS) is reported to alleviate oxidative stress and apoptosis, but its role in protecting aminoglycoside-induced hearing loss is unclear. In this study, we investigated the anti-oxidant and anti-apoptosis effect of NaHS in in vitro cultured House Ear Institute-Organ of Corti 1 (HEI-OC1) cells and isolated mouse cochlea. Results from cultured HEI-OC1 cells and cochlea consistently indicated that NaHS exhibited protective effects from gentamicin-induced ototoxicity, evident by maintained cell viability, hair cell number and cochlear morphology, reduced reactive oxygen species production and mitochondrial depolarization, as well as apoptosis activation of the intrinsic pathway. Moreover, in the isolated cochlear culture, NaHS was also demonstrated to protect the explant from gentamicin-induced mechanotransduction loss. Our study using multiple in vitro models revealed for the first time, the potential of NaHS as a therapeutic agent in protecting against aminoglycoside-induced hearing loss. PMID:26295804

  14. A3-Coupling catalyzed by robust Au nanoparticles covalently bonded to HS-functionalized cellulose nanocrystalline films

    Directory of Open Access Journals (Sweden)

    Jian-Lin Huang

    2013-07-01

    Full Text Available We decorated HS-functionalized cellulose nanocrystallite (CNC films with monodisperse Au nanoparticles (AuNPs to form a novel nanocomposite catalyst AuNPs@HS-CNC. The uniform, fine AuNPs were made by the reduction of HAuCl4 solution with thiol (HS- group-functionalized CNC films. The AuNPs@HS-CNC nanocomposites were examined by X-ray photoelectron spectroscopy (XPS, TEM, ATR-IR and solid-state NMR. Characterizations suggested that the size of the AuNPs was about 2–3 nm and they were evenly distributed onto the surface of CNC films. Furthermore, the unique nanocomposite Au@HS-CNC catalyst displayed high catalytic efficiency in promoting three-component coupling of an aldehyde, an alkyne, and an amine (A3-coupling either in water or without solvent. Most importantly, the catalyst could be used repetitively more than 11 times without significant deactivation. Our strategy also promotes the use of naturally renewable cellulose to prepare reusable nanocomposite catalysts for organic synthesis.

  15. Effect of sludge retention time and phosphorus to carbon ratio on biological phosphorus removal in HS-SBR process.

    Science.gov (United States)

    Zhu, Rui; Wu, Min; Yang, Jian

    2013-01-01

    Sludge retention time (SRT) and phosphorus to carbon ratio (P/C) in the feed are important control parameters in biological phosphorus removal. In this study, humus soil sequencing batch reactor (HS-SBR) process was operated with different SRTs (five, 10 and 15 days) and P/C feeding (0.0125 and 0.1) to evaluate their phosphorus removal efficiencies. The HS-SBR was composed of a humus soil reactor (HSR) and a conventional SBR (designated as hsSBR to differentiate from the conventional SBR used as a control). The results showed that the phosphorus removal efficiency was 82.7%, 97.3% and 97.3% at SRTs of five, 10 and 15 days respectively and acetate utilization efficiency for phosphorus release with SRTs of 10 and 15 days was much higher than that with an SRT of five days. In addition, a high P/C feeding (0.1) could promote the growth of the phosphate accumulating organisms in the hsSBR; however, the efficiency of phosphorus removal was lower than a low P/C feeding (0.0125) at an SRT of 15 days. All these observations suggested that a relatively long SRT and low P/C feeding exert a useful effect on the phosphorus removal in the hsSBR.

  16. THE IMPLICATION OF PERIODONTITIS TO ATHEROSCLEROSIS: ITS RELATIONSHIP WITH LIPID PROFILE AND hs-CRP SERUM CONCENTRATION

    Directory of Open Access Journals (Sweden)

    Rasmidar Samad

    2015-06-01

    Full Text Available C-Reactive Protein and serum lipids are correlated with atherosclerosis. The purpose of this study was to prove that increasing level of hs-CRP and serum lipids were correlated with periodontitis. This was a cross-sectional approach with 63 samples. Periodontitis was determined if the pocket depth (PD was more than 4 mm and the attachment loss (AL was more than 2 mm. Serum lipids and hs-CRP was analyzed by enzymatic method and chemiluminescent (Immulite hs_CRP alternatively. With unpaired t-test it has showed that there was a difference level of hs-CRP and total cholesterol in periodontitis and non-periodontitis. It was found that there was a significant positive correlation between PD (r=0.26, AL (r=0.25 with hs-CRP serum level; and PD (r=0.27, AL (r=0.30 with total cholesterol and LDL. It was concluded that there is a possibility that there is a relationship between periodontitis and atherosclerosis.

  17. NaHS Protects against the Impairments Induced by Oxygen-Glucose Deprivation in Different Ages of Primary Hippocampal Neurons

    Science.gov (United States)

    Yu, Qian; Wang, Binrong; Zhao, Tianzhi; Zhang, Xiangnan; Tao, Lei; Shi, Jinshan; Sun, Xude; Ding, Qian

    2017-01-01

    Brain ischemia leads to poor oxygen supply, and is one of the leading causes of brain damage and/or death. Neuroprotective agents are thus in great need for treatment purpose. Using both young and aged primary cultured hippocampal neurons as in vitro models, we investigated the effect of sodium hydrosulfide (NaHS), an exogenous donor of hydrogen sulfide, on oxygen-glucose deprivation (OGD) damaged neurons that mimick focal cerebral ischemia/reperfusion (I/R) induced brain injury. NaHS treatment (250 μM) protected both young and aged hippocampal neurons, as indicated by restoring number of primary dendrites by 43.9 and 68.7%, number of dendritic end tips by 59.8 and 101.1%, neurite length by 36.8 and 66.7%, and spine density by 38.0 and 58.5% in the OGD-damaged young and aged neurons, respectively. NaHS treatment inhibited growth-associated protein 43 downregulation, oxidative stress in both young and aged hippocampal neurons following OGD damage. Further studies revealed that NaHS treatment could restore ERK1/2 activation, which was inhibited by OGD-induced protein phosphatase 2 (PP2A) upregulation. Our results demonstrated that NaHS has potent protective effects against neuron injury induced by OGD in both young and aged hippocampal neurons.

  18. A3-Coupling catalyzed by robust Au nanoparticles covalently bonded to HS-functionalized cellulose nanocrystalline films

    Science.gov (United States)

    Huang, Jian-Lin

    2013-01-01

    Summary We decorated HS-functionalized cellulose nanocrystallite (CNC) films with monodisperse Au nanoparticles (AuNPs) to form a novel nanocomposite catalyst AuNPs@HS-CNC. The uniform, fine AuNPs were made by the reduction of HAuCl4 solution with thiol (HS-) group-functionalized CNC films. The AuNPs@HS-CNC nanocomposites were examined by X-ray photoelectron spectroscopy (XPS), TEM, ATR-IR and solid-state NMR. Characterizations suggested that the size of the AuNPs was about 2–3 nm and they were evenly distributed onto the surface of CNC films. Furthermore, the unique nanocomposite Au@HS-CNC catalyst displayed high catalytic efficiency in promoting three-component coupling of an aldehyde, an alkyne, and an amine (A3-coupling) either in water or without solvent. Most importantly, the catalyst could be used repetitively more than 11 times without significant deactivation. Our strategy also promotes the use of naturally renewable cellulose to prepare reusable nanocomposite catalysts for organic synthesis. PMID:23946833

  19. Correlation of gene polymorphism and plasma contents of MBL and hs-CRP in patients with coronary heart disease%冠心病与MBL基因多态性及血浆MBL和hs-CRP水平的相关性

    Institute of Scientific and Technical Information of China (English)

    蔡念光; 王玉凤; 王晓明; 程健君; 贾天军; 吕占军

    2011-01-01

    Objective To analyze the correlation between coronary heart disease(CHD) and the gene polymorphism of mannan binding lectin(MBL), the plasma contents of MBL and the level of high-sensitivity C-reactive protein (hs-CRP).Methods The anti-coagulated blood of CHD patients (group CHD, 100 cases) and healthy people(group C,60 cases) was collected.The point mutation of MBL ExonI 54 codon was detected by PCR-RFLP.The content of MBL and the level of hs-CRP were detected by ELISA kit and immunoturbidimetry, respectively.Results There was no significant difference in the genotypes of MBL ExonI 54 codon between group CHD and group C(P>0.05).The plasma content of MBL and the level of hs-CRP in group CHD were higher than those in group C (P<0.01).In group CHD, there was no obvious difference in the content of MBL between the patients with high level of hs-CRP(>3 mg/L) and those with normal level of hs-CRP(<3 mg/L) (P>0.05).Conclusion MBL and hs-CRP may be involved in the occurrence and development of CHD via different mechanisms.%目的 研究冠心病(CHD)与甘露聚糖结合凝集素(MBL)基因多态性及其血浆MBL和高敏G-反应蛋白(hs-CRP)水平的关系.方法 收集100例CHD患者(CHD组)和60例健康人群(对照组)抗凝血,限制片段长度多态性法(PCR-RFLP)检测MBL ExonI 54位密码子基因点突变,ELISA法和免疫比浊法分别检测血浆MBL含量和高敏G-反应蛋白(hs-CRP)水平.结果 CHD组与对照组MBL基因ExonI 54位密码子点突变频率比较差异无统计学意义(P>0.05).CHD组血浆MBI和hs-CRP含量均高于对照组(P<0.01).而CHI组内血浆hs-CRP水平升高者(>3 mg/L)与hs-CRP水平正常者(<3 mg/L)MBL含量比较差异无统计学意义(P>0.05).结论 MBL和hs-CRP可能以不同机制参与CHD发生、发展过程.

  20. RoHS directive: restriction of the use of lead in electronic equipment

    CERN Multimedia

    2007-01-01

    The European Restriction of Hazardous Substances (RoHS) directive, which entered into force on 1st July 2006, restricts the use of lead in the manufacture of electronic equipment. This has many consequences for the manufacture of printed circuit boards and assemblies, from their design and repair to the purchase of components. If you have any questions on this subject, we invite you to attend a training session which will be held at CERN from 1.30 to 5.30 p.m. on 21st June. All the details, including the course contents and instructions on how to register for it, can be found here. Please note that the session will be held in French.

  1. RoHS directive: restriction of the use of lead in electronic equipment

    CERN Multimedia

    2007-01-01

    The European Restriction of Hazardous Substances (RoHS) directive, which entered into force on 1st July 2006, restricts the use of lead in the manufacture of electronic equipment. This has many consequences for the manufacture of printed circuit boards and assemblies, from their design and repair to the purchase of components. If you have any questions on this subject, we invite you to attend a training session which will be held at CERN from 1.30 to 5.30 to p.m. on 21st June. All the details, including the course contents and instructions on how to register for it, can be found here Please note that the session will be held in French.

  2. Danish WEEE management in the light of the WEEE and RoHS Directives: a quantitative analysis of critical issues

    DEFF Research Database (Denmark)

    Pizzol, Massimo; Thomsen, Marianne

    Waste from Electrical and Electronic Equipment (WEEE) is the fastest growing waste fraction in Europe and is classified as hazardous as it may include substances that have adverse effects on human health or cause damage to the environment (UNEP, 2009). The European Community Directive 2002/96/EC...... on Waste Electrical and Electronic Equipment (WEEE Directive), together with the European Community Directive 2002/95/EC on the Restriction of Hazardous Substances (RoHS Directive) were established respectively to set collection, recycling and recovery targets, and to restrict the use of six hazardous......HS directive, flows of toxic compounds are still significant in WEEE, as can be demonstrated by performing a Substance Flow Analysis (SFA) (Brunner, 2004) on a Danish WEEE treatment facility. This is due to the presence, in the WEEE, of devices constituting an exception to the RoHS directive and of other...

  3. RoHS regulated substances in mixed plastics from waste electrical and electronic equipment.

    Science.gov (United States)

    Wäger, Patrick A; Schluep, Mathias; Müller, Esther; Gloor, Rolf

    2012-01-17

    The disposal and recovery of plastics from waste electrical and electronic equipment (WEEE) are of considerable importance, both from an environmental and an economic perspective. This paper presents the results of a study investigating current concentrations of hazardous substances in mixed plastics from WEEE and their implications for an environmentally sound recovery. The study included 53 sampling campaigns for mixed plastics from WEEE. The samples were analyzed with regard to heavy metals (cadmium, chromium, mercury, and lead) and flame retardants (PentaBDE, OctaBDE, DecaBDE, DecaBB) regulated in the RoHS Directive. Besides these substances, other brominated flame retardants known to occur in electronics (HBCD, TBBPA) as well as the total bromine and phosphorus contents were considered. Results show that no mixed plastics fraction from WEEE is completely free from substances regulated in the RoHS Directive. The lowest number and average concentrations were found in flat screen monitors. The highest concentrations were found in mixed plastics from CRT monitors and TVs. Mixed plastics fractions with high average concentrations of heavy metals originate from the treatment of small household appliances (cadmium), ICT equipment (lead), and consumer equipment (lead). Mixed plastics fractions with high average concentrations of brominated flame retardants mainly originate from the treatment of small household appliances for high temperature applications (DecaBDE), CRT monitors (OctaBDE and DecaBDE) and consumer equipment (DecaBDE), in particular CRT TVs (DecaBDE). To avoid a dissipation of hazardous substances into plastics and the environment, it is recommended that mixed plastics from WEEE are subject to a strict quality management.

  4. Revealing fibrinogen monolayer conformations at different pHs: electrokinetic and colloid deposition studies.

    Science.gov (United States)

    Nattich-Rak, Małgorzata; Adamczyk, Zbigniew; Wasilewska, Monika; Sadowska, Marta

    2015-07-01

    Adsorption mechanism of human fibrinogen on mica at different pHs is studied using the streaming potential and colloid deposition measurements. The fibrinogen monolayers are produced by a controlled adsorption under diffusion transport at pH of 3.5 and 7.4. Initially, the electrokinetic properties of these monolayers and their stability for various ionic strength are determined. It is shown that at pH 3.5 fibrinogen adsorbs irreversibly on mica for ionic strength range of 4×10(-4) to 0.15 M. At pH 7.4, a partial desorption is observed for ionic strength below 10(-2) M. This is attributed to the desorption of the end-on oriented molecules whereas the side-on adsorbed molecules remain irreversibly bound at all ionic strengths. The orientation of molecules and monolayer structure is evaluated by the colloid deposition measurements involving negatively charged polystyrene latex microspheres, 820 nm in diameter. An anomalous deposition of negative latex particles on substrates exhibiting a negative zeta potential is observed. At pH 3.5 measurable deposition of latex is observed even at low ionic strength where the approach distance of latex particles exceeded 70 nm. At pH 7.4 this critical distance is 23 nm. This confirms that fibrinogen monolayers formed at both pHs are characterized by the presence of the side-on and end-on oriented molecules that prevail at higher coverage range. It is also shown that positive charge is located at the end parts of the αA chains of the adsorbed fibrinogen molecules. Therefore, it is concluded that the colloid deposition method is an efficient tool for revealing protein adsorption mechanisms at solid/electrolyte interfaces.

  5. Synthesis and pharmacological evaluation of [(3)H]HS665, a novel, highly selective radioligand for the kappa opioid receptor.

    Science.gov (United States)

    Guerrieri, Elena; Mallareddy, Jayapal Reddy; Tóth, Géza; Schmidhammer, Helmut; Spetea, Mariana

    2015-03-18

    Herein we report the radiolabeling and pharmacological investigation of a novel radioligand, the N-cyclobutylmethyl substituted diphenethylamine [(3)H]HS665, designed to bind selectively to the kappa opioid peptide (KOP) receptor, a target of therapeutic interest for the treatment of a variety of human disorders (i.e., pain, affective disorders, drug addiction, and psychotic disorders). HS665 was prepared in tritium-labeled form by a dehalotritiated method resulting in a specific activity of 30.65 Ci/mmol. Radioligand binding studies were performed to establish binding properties of [(3)H]HS665 to the recombinant human KOP receptor in membranes from Chinese hamster ovary cells stably expressing human KOP receptors (CHOhKOP) and to the native neuronal KOP receptor in guinea pig brain membranes. Binding of [(3)H]HS665 was specific and saturable in both tissue preparations. A single population of high affinity binding sites was labeled by [(3)H]HS665 in membranes from CHOhKOP cells and guinea pig brain with similar equilibrium dissociation constants, Kd, 0.45 and 0.64 nM, respectively. Average receptor density of [(3)H]HS665 recognition sites were 5564 and 154 fmol/mg protein in CHOhKOP cells and guinea pig brain, respectively. This study shows that the new radioligand distinguishes and labels KOP receptors specifically in neuronal and cellular systems expressing KOP receptors, making this molecule a valuable tool in probing structural and functional mechanisms governing ligand-KOP receptor interactions in both a recombinant and native in vitro setting.

  6. The impact of cHS4 insulators on DNA transposon vector mobilization and silencing in retinal pigment epithelium cells.

    Directory of Open Access Journals (Sweden)

    Nynne Sharma

    Full Text Available DNA transposons have become important vectors for efficient non-viral integration of transgenes into genomic DNA. The Sleeping Beauty (SB, piggyBac (PB, and Tol2 transposable elements have distinct biological properties and currently represent the most promising transposon systems for animal transgenesis and gene therapy. A potential obstacle, however, for persistent function of integrating vectors is transcriptional repression of the element and its genetic cargo. In this study we analyze the insulating effect of the 1.2-kb 5'-HS4 chicken β-globin (cHS4 insulator element in the context of SB, PB, and Tol2 transposon vectors. By examining transgene expression from genomically inserted transposon vectors encoding a marker gene driven by a silencing-prone promoter, we detect variable levels of transcriptional silencing for the three transposon systems in retinal pigment epithelium cells. Notably, the PB system seems less vulnerable to silencing. Incorporation of cHS4 insulator sequences into the transposon vectors results in 2.2-fold and 1.5-fold increased transgene expression levels for insulated SB and PB vectors, respectively, but an improved persistency of expression was not obtained for insulated transgenes. Colony formation assays and quantitative excision assays unveil enhanced SB transposition efficiencies by the inclusion of the cHS4 element, resulting in a significant increase in the stable transfection rate for insulated SB transposon vectors in human cell lines. Our findings reveal a positive impact of cHS4 insulator inclusion for SB and PB vectors in terms of increased transgene expression levels and improved SB stable transfection rates, but also the lack of a long-term protective effect of the cHS4 insulator against progressive transgene silencing in retinal pigment epithelium cells.

  7. Relationship of serum levels of hs-CRP and Hcy with gestational diabetes mellitus and pregnancy outcomes%血清hs-CRP、Hcy与妊娠期糖尿病及妊娠结局的关系

    Institute of Scientific and Technical Information of China (English)

    徐旭赟; 黄美英

    2015-01-01

    Objective To evaluate the value of combined detection of high sensitivity C-reactive protein ( hs-CRP ) and homocysteine (Hcy) levels in diagnosing gestational diabetes mellitus (GDM) and its value on predicting pregnancy outcomes. Methods Thirty-five women with GDM accepting pre-natal screening in Third People’ s Hospital of Anji County from January 2012 to March 2014 were selected in observation group. Another 35 healthy women diagnosed without GDM at the same period were selected in control group. The levels of hs-CRP, Hcy, fasting plasma glucose ( FPG) and fasting insulin ( FINS) in two groups were detected and compared, and the pregnancy outcomes were followed up. Results The levels of FPG, FINS, hs-CRP and Hcy in the observation group were higher than those in the control group, and the differences were significant (t value was 13. 07, 12. 02, 3. 65 and 12. 02, respectively, all P <0. 05). The incidence of adverse reactions was 34. 3% (12/35) and 11. 4% (4/35) in the observation group and the control group, respectively with statistical significance (χ2 =5. 19,P<0. 05). Sixteen cases were found with adverse pregnancy outcomes, and their hs-CRP and Hcy levels were higher than those of 54 normal pregnant women (t value was 7. 88 and 14. 17, respectively, both P<0. 05). Conclusion The levels of hs-CRP and Hcy are closely related with GDM, and the high levels have predictive value for adverse pregnancy outcomes. Therefore, attention should be attracted on them.%目的:探讨联合检测超敏C反应蛋白( hs-CRP)和同型半胱氨酸( Hcy)水平在妊娠期糖尿病( GDM)诊断及对妊娠结局的预测价值。方法选择2012年1月至2014年3月在安吉县第三人民医院门诊接受检查的GDM患者35例作为观察组;选择同期经临床排除GDM的正常孕产妇35例作为对照组。检测两组患者的hs-CRP和Hcy、空腹血糖和胰岛素水平,并跟踪两组妊娠结局。结果观察组患者空腹血糖、胰岛素、hs-CRP 和 Hcy

  8. 冠心病血清 hs_CRP、ox_LDL及 Hcy 水平的研究%Study of Levels of Serum hs CRP,ox LDL and Hcy in Patients with Coronary Heart Disease

    Institute of Scientific and Technical Information of China (English)

    李方江; 王晓元; 张文婷; 杜美玲; 李清; 张强; 李慧敏

    2015-01-01

    目的:探讨冠心病患者外周血高敏C反应蛋白(hs CRP )、氧化型低密度脂蛋白(ox LDL )和同型半胱氨酸(Hcy )水平及其意义。方法选取经冠状动脉造影检查证实冠心病组272例,分为急性心肌梗死(AMI )组105例,不稳定型心绞痛(UA )组126例,稳定型心绞痛(SAP )组41例;选取冠状动脉造影证实冠状动脉无狭窄病变56例为对照组。采集研究对象入院24小时内静脉血,ELISA法检测ox LDL水平,日立7070型全自动生化分析仪测定hs CRP、Hcy、总胆固醇(TC )、甘油三脂(TG )、低密度脂蛋白(LDL )指标。结果冠心病组血清hs CRP、ox LDL、Hcy水平显著高于对照组,差异有统计学意义( P<0.01);AMI组血清hs CRP、ox LDL、Hcy水平显著高于SAP组,差异有统计学意义( P<0.05);AMI组血清hs CRP、ox LDL水平显著高于UA组,差异有统计学意义( P<0.05)。结论联合检测hs CRP、ox LDL、Hcy有助于对冠心病的诊断。%Objective To investigate the level and significance of peripheral high sensitivity C reactive protein (hs CRP ) ,oxidized low density lipoprotein (ox LDL ) and homocysteine (Hcy ) in patients with coronary heart disease. Methods 272 patients with coro-nary heart disease were selected ,which confirmed by coronary angiography , including 105 cases of acute myocardial infarction (AMI) ,126 cases of unstable angina (UA) and 41 cases of stable angina patients (SAP). The control group included 56 patients with no coronary artery stenosis confirmed by coronary angiography . Collected the fasting blood of patients within 24 hours of admis-sion ,the levels of ox LDL was detected by ELISA ,and the determination of hs CRP、Hcy、total cholesterol ,triglycerides ,LDL index was detected by Hitachi 7070 automatic biochemical analyzer. Results The serum levels of hs CRP、ox LDL、Hcy in the grope of coro-nary heart disease were significantly higher than

  9. Redox chemistry of H[sub 2]S oxidation by the British Gas Stretford Process. Pt. 2; Electrochemical behaviour of aqueous hydrosulphide (HS[sup -]) solutions

    Energy Technology Data Exchange (ETDEWEB)

    Kelsall, G.H.; Thompson, I. (Imperial College, London (United Kingdom). Dept. of Mineral Resources Engineering)

    1993-04-01

    Electrochemical techniques were used to study the oxidation of HS[sup -] ions at pH 9.3. Voltammetry of gold electrodes in HS[sup -]-containing solutions showed that multilayers of sulphur and soluble oxidation products were formed. As a known HS[sup -] oxidation product, thiosulphate solutions were also studied voltammetrically, but found to be electro-inactive at mildly oxidising potentials. The voltammetric behaviour of polysulphide ions, S[sub n][sup 2-] (n = 2 to 5), was similar to that of HS[sup -] solutions on oxidation, though they could be reduced to HS[sup -] ions at low potentials. Ring-disc electrode experiments, extending the HS[sup -] concentration range that had been studied previously, confirmed that polysulphide ions were produced on reduction of anodically deposited elemental sulphur. This was demonstrated in both cyclic voltammetry and potential step experiments. (author)

  10. HS-SPME-GC-MS分析螺旋藻挥发性成分%Analysis of Volatile Components of Spirulina by HS-SPME-GC-MS

    Institute of Scientific and Technical Information of China (English)

    张丽君; 许柏球; 王金林; 罗欢忠; 陈晚霞; 林伟强

    2013-01-01

      The Spirulina platensis was selected as material and the odor flavor components was analyzed .The volatile components was determined by Headspace solid phase microextraction combined with gas hromatography mass spectrometry (HS-SPME-GC-MS).The results showd that 17 volatile components were indentified,including alkanes (relative content 79.77%), aldehydes and ketones (relative content 18.27%), alcohols (relative content 0.84 %) and aromatic compounds (relative content 1.17 %). The main volatile components of Spirulina platensis were Heptadecane (relative content 64.52 %), Pentadecane ( relative content8.59%), Dihydroactinidiolide (relative content 7.21%) ,β-ionone ( relative content 6.65%) and Hexadecane (relative content4.29%).%  以钝顶螺旋藻藻粉为原料,对其腥味物质进行分析。采用顶空固相微萃取和气相色谱-质谱联用技术,对钝顶螺旋藻的挥发性成分进行检测,共鉴定出17种有机化合物,烷烃类化合物(相对含量79.77%)、醛酮类化合物(相对含量18.27%)、醇类化合物(相对含量0.84%)、芳香族化合物(相对含量1.17%)。螺旋藻的主要挥发成分依次为十七烷(平均相对含量为64.52%)、十五烷(平均相对含量为8.59%)、二氢猕猴桃内酯(平均相对含量为7.21%)、β-紫罗兰酮(平均相对含量为6.65%)和十六烷(平均相对含量为4.29%)。

  11. Effect of autoclave heat treatments on the mechanical properties of the prealloyed powder cobalt-base alloy HS-31

    Science.gov (United States)

    Freche, J. C.; Ashbrook, R. L.

    1973-01-01

    The cobalt-base alloy HS-31 was atomized into powder and then consolidated by extrusion or by hot isostatic pressing (HIP) in an autoclave over a range of temperatures spanning the solidus, approximately 2340 F. Extrusions were subsequently autoclaved at the same conditions. Extrusions autoclaved at 2420 F had a life of 300 hours at 1200 F and 30 hours at 1800 F at stresses that result in a 10-hour life with cast HS-31. Superior stress rupture lives of autoclaved material are probably related to the solidification structure at the grain boundaries as well as to the increased grain size.

  12. Galectin 1 Proangiogenic and Promigratory Effects in the Hs683 Oligodendroglioma Model Are Partly Mediated through the Control of BEX2 Expression

    OpenAIRE

    Mercier, Marie Le; Fortin, Shannon; Mathieu, Véronique; Roland, Isabelle; Spiegl-Kreinecker, Sabine; Haibe-Kains, Benjamin; Bontempi, Gianluca; Decaestecker, Christine; Berger, Walter; Lefranc, Florence; Kiss, Robert

    2009-01-01

    We have previously reported that galectin 1 (Gal-1) plays important biological roles in astroglial as well as in oligodendroglial cancer cells. As an oligodendroglioma model, we make use of the Hs683 cell line that has been previously extensively characterized at cell biology, molecular biology, and genetic levels. Galectin 1 has been shown to be involved in Hs683 oligodendroglioma chemoresistance, neoangiogenesis, and migration. Down-regulating Gal-1 expression in Hs683 cells through targete...

  13. What Do Very Low Plasma Concentrations of High-sensitivity C-reactive Protein (hs-CRP) Mean among Healthy Middle-aged Koreans?

    OpenAIRE

    Kim, Min-Gul; Cho, Baik-Hwan; Chae, Soo-Wan; Park, Tae-Sun; Kim, Dal-Sik

    2015-01-01

    Background Systemic low-grade inflammation (SLGI), as assessed by measurements of high-sensitivity C-reactive protein (hs-CRP), is a strong independent risk factor for cardiovascular diseases (CVD). Although individuals with hs-CRP ≤ 1 mg/L have been defined as being at low risk according to AHA/CDC guidelines, the value of very low hs-CRP levels (

  14. 5'HS5 of the human beta-globin locus control region is dispensable for the formation of the beta-globin active chromatin hub.

    Directory of Open Access Journals (Sweden)

    Ping Kei Chan

    Full Text Available Hypersensitive site 5 (5'HS5 of the beta-globin Locus Control Region functions as a developmental stage-specific border in erythroid cells. Here, we have analyzed the role of 5'HS5 in the three dimensional organization of the beta-gene locus using the Chromatin Conformation Capture (3C technique. The results show that when 5'HS5 is deleted from the locus, both remote and internal regulatory elements are still able to interact with each other in a three-dimensional configuration termed the Active Chromatin Hub. Thus, the absence of 5'HS5 does not have an appreciable effect on the three dimensional organization of the beta-globin locus. This rules out models in which 5'HS5 nucleates interactions with remote and/or internal regulatory elements. We also determined the binding of CTCF, the only defined insulator protein in mammalian cells, to 5'HS5 by using chromatin immunoprecipitation (ChIP assays. We detect low levels of CTCF binding to 5'HS5 in primitive erythroid cells, in which it functions as a border element. Surprisingly, we also observe binding levels of CTCF to 5'HS5 in definitive erythroid cells. Thus, binding of CTCF to 5'HS5 per se does not render it a functional border element. This is consistent with the previous data suggesting that CTCF has dual functionality.

  15. 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline.

    Directory of Open Access Journals (Sweden)

    Wai Kit Chan

    Full Text Available Heparan sulfate (HS is a linear carbohydrate composed of polymerized uronate-glucosamine disaccharide units that decorates cell surface and secreted glycoproteins in the extracellular matrix. In mammals HS is subjected to differential sulfation by fifteen different heparan sulfotransferase (HST enzymes of which Hs2st uniquely catalyzes the sulfation of the 2-O position of the uronate in HS. HS sulfation is postulated to be important for regulation of signaling pathways by facilitating the interaction of HS with signaling proteins including those of the Fibroblast Growth Factor (Fgf family which signal through phosphorylation of extracellular signal-regulated kinases Erk1/2. In the developing mouse telencephalon Fgf2 signaling regulates proliferation and neurogenesis. Loss of Hs2st function phenocopies the thinned cerebral cortex of mutant mice in which Fgf2 or Erk1/2 function are abrogated, suggesting the hypothesis that 2-O-sulfated HS structures play a specific role in Fgf2/Erk signaling pathway in this context in vivo. This study investigated the molecular role of 2-O sulfation in Fgf2/Erk signaling in the developing telencephalic midline midway through mouse embryogenesis at E12.5. We examined the expression of Hs2st, Fgf2, and Erk1/2 activity in wild-type and Hs2st-/- mice. We found that Hs2st is expressed at high levels at the midline correlating with high levels of Erk1/2 activation and Erk1/2 activation was drastically reduced in the Hs2st-/- mutant at the rostral telencephalic midline. We also found that 2-O sulfation is specifically required for the binding of Fgf2 protein to Fgfr1, its major cell-surface receptor at the rostral telencephalic midline. We conclude that 2-O sulfated HS structures generated by Hs2st are needed to form productive signaling complexes between HS, Fgf2 and Fgfr1 that activate Erk1/2 at the midline. Overall, our data suggest the interesting possibility that differential expression of Hs2st targets the rostral

  16. LDL-C和hs-CRP在急性冠脉综合征诊断中的价值%Diagnosis value of LDL-C and hs-CRP in acute coronary syndrome

    Institute of Scientific and Technical Information of China (English)

    叶碧宁

    2012-01-01

    目的 分析研究低密度脂蛋白胆固醇(LDL-C)、血清高敏C 反应蛋白(hs-CRP)对急性冠脉综合征(ACS)的临床诊断价值.方法 选择2009年9月至2010年9月期间该院心内科收治的84例ACS患者作为研究组,并选择同时期门诊体检的84例健康者作为对照组,分别用免疫比浊法检测两组的血清hs-CRP水平,速率法检测LDL-C水平,并分析计算LDL-C和hs-CRP在诊断ACS方面的效能.结果 研究组血清LDL-C和hs-CRP的浓度明显高于对照组的(P<0.05).单独LDL-C诊断ACS的敏感性与特异性分别为51.2%和82.3%,阳性和阴性预测值分别为75.9%和55.3%.单独hs-CRP诊断ACS的敏感性与特异性分别为48.8%和85.6%,阳性和阴性预测值分别为73.8%和52.9%.LDL-C和hs-CRP联合诊断ACS的阳性例数为82例,与单独LDL-C诊断的阳性例数差异有显著性(χ2=6.41,P<0.05),与单独hs-CRP诊断的阳性例数也差异有显著性(χ2=6.63,P<0.05),敏感性与特异性分别为95.6%和46.3%,阳性和阴性预测值分别为65.6%和91.2%.结论 联合检测LDL-C和hs-CRP可有助于早期诊断ACS.%Objective To explore detection value of low-density lipoprotein cholesterol( LDL-C ) and high sensitive C-reactive protein ( hs-CRP ) in the diagnosis of acute coronary syndrome( ACS ). Methods Eighty-four cases of ACS as the research group were collected and 84 healthy people as the control group were collected. Serum hs-CRP levels of the two groups were tested by immune turbidimetry method, and LDL-C levels were tested by rate method. Efficiency of hs-CRP level and LDL-C level in the diagnosis of ACS was analyzed. Results The levels of LDL-C and hs-CRP of the research group were significantly higher than those in the control group, with P <0. 05. The sensitivity and specificity of LDL-C in the diagnosis of ACS was 51. 2% and 82. 3% , positive and negative predictive value was 75. 9% and 55. 3% . The sensitivity and specificity of hs-CRP in the diagnosis of ACS was 48. 8% and 85

  17. Levels and prognostic significance of NT-ProBNP,hs-CRP in chronic heart failure%慢性心力衰竭NT-ProBNP、hs-CRP变化及其预后价值

    Institute of Scientific and Technical Information of China (English)

    周金锋; 程自平

    2013-01-01

    目的 探讨B型钠尿肽前体(NT-ProBNP)和高敏C反应蛋白(hs-CRP)联合检测对心力衰竭诊断、病情评估和预后判断的价值.方法 测定该院2010年1月-2012年11月103例明确诊断为充血性心力衰竭(CHF)的患者,测定入院时、出院时血浆NT-ProBNP 和血清hs-CRP水平,进行治疗前后对比观察,并随机抽取该院35名健康体检者作对照组比较,随访6月观察患者心脏事件再发生的情况.结果 血浆NT-ProBNP和血清hs-CRP和水平在心衰不同心功能分级组和健康对照组之间差异有统计学意义,心功能越差,其浓度越高(P<0.05);治疗前后比较,差异均也有统计学意义(P<0.05);出院后发生心脏事件组血浆NT-ProBNP 和血清hs-CRP水平浓度明显高于未发生心脏事件组(P<0.05).结论 心衰患者中NT-ProBNP 、hs-CRP明显升高,二者联合检测对心衰诊断、病情评估和预后判断有应用意义.%Objective To observe the value of B-type natriuretic peptide precursor (NT -ProBNP)and high-sensitivity C-eactive protein (hs-CRP)on patients of heart failure in diagnosis , illness evaluation and prognosis through jointed detection . Methods From January 2010 to November 2012,103 cases of CHF patients were measured plasma NT -ProBNP and hs -CRP serum levels on admission and at discharge. Before and after treatment comparison observations were made ,35 health were selected randomly for control. The patients were followed up for 6 months to observe it cardiac events happen again . Results NT-ProBNP and hs -CRP serum and plasma levels in different heart failure cardiac function grading group had statistically significant difference from healthy control group . The poorer heart function , the higher concentration ( P < 0.05 ) ; Before and after treatment, difference had statistical significance ( P < 0.05 ) ; Concentrations of plasma NT -ProBNP and hs -CRP level were significantly higher in cardiac evento after discharge group than those without cardiac

  18. hs-CRP与肥胖青少年心血管疾病发生风险的相关性研究%hs-CRP level was associated to diagnosis of cardiovascular risk in obese teenagers

    Institute of Scientific and Technical Information of China (English)

    张丽娜; 寇筱囡; 李新娜; 曹艳菲; 车璐

    2014-01-01

    目的:探讨超敏C反应蛋白(high-sensitivity C-reactive protein, hs-CRP)作为肥胖青少年心血管疾病发病风险的生物标志物的评估价值。方法6-18岁青少年,体重指数(body mass index, BMI)超过2 SDS。所有入组对象空腹12h采集静脉血,检测hs-CRP,肝脏功能检测,血脂检测,尿酸等检测;糖耐量试验。我们认为肥胖青少年存在代谢综合征症状,至少包括两个以下症状:高甘油三酯血症、低高密度脂蛋白胆固醇(high density lipoprotein cholesterol, HDL-C)、高血压及糖代谢障碍。结果115例青少年患者中,24%显示存在代谢综合症,存在代谢综合症的患者血清hs-CRP水平(M=3.8ml/L;95% CI:2.9-4.8)高与不存在代谢综合征症状的肥胖青少年(M=1.9ml/L;95% CI:1.5-2.5),经多变量分析,影响hs-CRP水平的是BMI、甘油三酯、HDL-C水平。讨论血清hs-CRP是判断肥胖青少年是否存在早期心血管疾病发生风险的有价值的生物标志物。%Objective To assess the utility of the high -sensitivity C-reactive protein (hs-CRP) as a marker of cardiovascular risk in obese teenagers. Methods The study included teenagers between 6 and 18 years of age with a body mass index (BMI) higher than 2 SDS. Al the patients had their blood pressure taken and hs-CRP, hepatic function, lipid and uric acid were determined after 12 h of fasting, likewise , an oral glucose tolerance test was performed, determining basal glucose and insulin levels, and after stimulus. Results Out of the 115 obese teenagers 24% showed signs of metabolic syndrome. Those with metabolic syndrome presented higher levels of hs-CRP (M=3.8ml/L; 95% CI: 2.9-4.8) in comparison with the obese patients who did not show signs of metabolic syndrome(M=1.9ml/L; 95% CI: 1.5-2.5). After a multivariate analysis, the variables that appear to influence the changes in hs-CRP were BMI, triglycerides and HDL-C levels. Conclusions The hs-CRP is

  19. Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening.

    Science.gov (United States)

    Ma, Yi; Ghoshdastider, Umesh; Wang, Jufang; Ye, Wei; Dötsch, Volker; Filipek, Slawomir; Bernhard, Frank; Wang, Xiaoning

    2012-12-01

    Glucosamine 6-phosphate N-acetyltransferase (GNA1; EC 2.3.1.4) is required for the de novo synthesis of N-acetyl-d-glucosamine-6-phosphate (GlcNAc-6P), which is an essential precursor in Uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) biosynthesis pathway. Therefore, GNA1 is indispensable for the viability of organisms. Here, a novel cell-free expression strategy was developed to efficiently produce large amounts of human GNA1(HsGNA1) and HsGNA1-sGFP for throughput inhibitor screening. The binding site of inhibitor glucose-6-phosphate (G6P) to hGNA was identified by simulated annealing. Subtle differences to the binding site of Aspergillius GNA1(AfGNA1) can be harnessed for inhibitor design. HsGNA1 may be also useful as an antimicrobial and chemotherapeutic target against cancer. Additionally HsGNA1 inhibitors/modulators can possibly be administered with other drugs in the next generation of personalized medicine.

  20. Heat Perception and Aversive Learning in Honey Bees: Putative Involvement of the Thermal/Chemical Sensor AmHsTRPA.

    Science.gov (United States)

    Junca, Pierre; Sandoz, Jean-Christophe

    2015-01-01

    The recent development of the olfactory conditioning of the sting extension response (SER) has provided new insights into the mechanisms of aversive learning in honeybees. Until now, very little information has been gained concerning US detection and perception. In the initial version of SER conditioning, bees learned to associate an odor CS with an electric shock US. Recently, we proposed a modified version of SER conditioning, in which thermal stimulation with a heated probe is used as US. This procedure has the advantage of allowing topical US applications virtually everywhere on the honeybee body. In this study, we made use of this possibility and mapped thermal responsiveness on the honeybee body, by measuring workers' SER after applying heat on 41 different structures. We then show that bees can learn the CS-US association even when the heat US is applied on body structures that are not prominent sensory organs, here the vertex (back of the head) and the ventral abdomen. Next, we used a neuropharmalogical approach to evaluate the potential role of a recently described Transient Receptor Potential (TRP) channel, HsTRPA, on peripheral heat detection by bees. First, we applied HsTRPA activators to assess if such activation is sufficient for triggering SER. Second, we injected HsTRPA inhibitors to ask whether interfering with this TRP channel affects SER triggered by heat. These experiments suggest that HsTRPA may be involved in heat detection by bees, and represent a potential peripheral detection system in thermal SER conditioning.

  1. Time delay in the variability of multiply lensed QSOs HS0810+2554 and Q2237+030

    Science.gov (United States)

    Storrs, Alex; Lainez, Sergio

    2017-01-01

    We present time delay in variability between the brightest lensed images of two multiply lensed QSO systems, HS0810+2554 and Q2237+030. This data can help constrain the mass distribution of the lensing galaxies, and perhaps on Hubble’s Constant as well. Observations were made with the Towson University 0.4m telescope.

  2. Complete genomic sequence of Campylobacter jejuni subsp. jejuni HS:19 strain RM1285 that was isolated from packaged chicken

    Science.gov (United States)

    Poultry products serve as the main source of Campylobacter jejuni subsp. jejuni (Cjj) infections in humans. Cjj infections are a leading cause of foodborne gastroenteritis and are a prevalent antecedent to Guillain-Barré syndrome (GBS). This study describes the genome of Cjj HS:19 strain RM1285 isol...

  3. Chronic NaHS Treatment Is Vasoprotective in High-Fat-Fed ApoE−/− Mice

    Directory of Open Access Journals (Sweden)

    Asha Ford

    2013-01-01

    Full Text Available Hydrogen sulfide is emerging as an important mediator of vascular function that has antioxidant and cytoprotective effects. The aim of this study was to investigate the role of endogenous H2S and the effect of chronic exogenous H2S treatment on vascular function during the progression of atherosclerotic disease. ApoE−/− mice were fed a high-fat diet for 16 weeks and treated with the H2S donor NaHS or the cystathionine-γ-lyase (CSE inhibitor D,L-propargylglycine (PPG, to inhibit endogenous H2S production for the final 4 weeks. Fat-fed ApoE−/− mice displayed significant aortic atherosclerotic lesions and significantly impaired endothelial function compared to wild-type mice. Importantly, 4 weeks of NaHS treatment significantly reduced vascular dysfunction and inhibited vascular superoxide generation. NaHS treatment significantly reduced the area of aortic atherosclerotic lesions and attenuated systolic blood pressure. Interestingly, inhibiting endogenous, CSE-dependent H2S production with PPG did not exacerbate the deleterious vascular changes seen in the untreated fat-fed ApoE−/− mice. The results indicate NaHS can improve vascular function by reducing vascular superoxide generation and impairing atherosclerotic lesion development. Endogenous H2S production via CSE is insufficient to counter the atherogenic effects seen in this model; however exogenous H2S treatment has a significant vasoprotective effect.

  4. Development of a Direct Headspace Collection Method from Arabidopsis Seedlings Using HS-SPME-GC-TOF-MS Analysis

    Directory of Open Access Journals (Sweden)

    Kazuki Saito

    2013-04-01

    Full Text Available Plants produce various volatile organic compounds (VOCs, which are thought to be a crucial factor in their interactions with harmful insects, plants and animals. Composition of VOCs may differ when plants are grown under different nutrient conditions, i.e., macronutrient-deficient conditions. However, in plants, relationships between macronutrient assimilation and VOC composition remain unclear. In order to identify the kinds of VOCs that can be emitted when plants are grown under various environmental conditions, we established a conventional method for VOC profiling in Arabidopsis thaliana (Arabidopsis involving headspace-solid-phase microextraction-gas chromatography-time-of-flight-mass spectrometry (HS-SPME-GC-TOF-MS. We grew Arabidopsis seedlings in an HS vial to directly perform HS analysis. To maximize the analytical performance of VOCs, we optimized the extraction method and the analytical conditions of HP-SPME-GC-TOF-MS. Using the optimized method, we conducted VOC profiling of Arabidopsis seedlings, which were grown under two different nutrition conditions, nutrition-rich and nutrition-deficient conditions. The VOC profiles clearly showed a distinct pattern with respect to each condition. This study suggests that HS-SPME-GC-TOF-MS analysis has immense potential to detect changes in the levels of VOCs in not only Arabidopsis, but other plants grown under various environmental conditions.

  5. Do albuminuria and hs-CRP add to the International Diabetes Federation definition of the metabolic syndrome in predicting outcome?

    NARCIS (Netherlands)

    van der Velde, Marije; Bello, Aminu K.; Brantsma, Auke H.; El Nahas, Meguid; Bakker, Stephan J. L.; de Jong, Paul E.; Gansevoort, Ronald T.

    2012-01-01

    Background. To investigate the added value of elevated urinary albumin excretion (UAE) and high high-sensitive C-reactive protein (hs-CRP) in predicting new-onset type 2 diabetes mellitus (T2DM), cardiovascular disease (CVD) and chronic kidney disease (CKD) in addition to the present metabolic syndr

  6. Correlation of Ferritin and Transferrin Serum with hsCRP and F2-Isoprostane in Metabolic Syndrome

    Directory of Open Access Journals (Sweden)

    Waode Nurfina

    2010-12-01

    Full Text Available BACKGROUND: The low inflammatory state that accompanies the Metabolic Syndrome (MetS associates with the overexpression of oxidative stress. Ferritin and Transferrin serum are often used to measure iron status and their concentrations are altered in several metabolic conditions. We hypothesized that concentration of Ferritin and Transferrin serum increase in Metabolic Syndrome (MetS and correlate with the inflammation and oxidative stress. METHODS: We studied 65 male MetS patients, aged 43.26±7.16 years. Iron metabolism was measured by concentration of Ferritin and Transferrin serums, while inflammatory and oxidative stress by high sensitivity C-reactive Protein (hsCRP and F2-Isoprostane. RESULTS: Concentration of Ferritin 315.70±188.63 ng/L and Transferrin 2.36±0.31 g/L increased along with increasing components of MetS. Concentration of Ferritin serum had a positive correlation with hsCRP (r=0.220 and F2-Isoprostane (r=0.023. CONCLUSIONS: Serum concentration of Ferritin increased in the MetS and correlates with hsCRP and F2-Isoprostane. KEYWORDS: metabolic syndrome, ferritin, transferrin, hsCRP, F2-isoprostane.

  7. Study to evaluate serum free testosterone and hsCRP concentration to predict low hematocrit in type 2 diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Radhey Shyam Chejara

    2015-06-01

    Conclusion: At the end of the study we concluded that both a low serum free testosterone level and high hs-CRP concentration may play an important role in the pathogenesis of mild anemia and low hematocrit values in DM type 2 patients. [Int J Res Med Sci 2015; 3(6.000: 1501-1504

  8. A new naphthalenepropanoic acid analog from the marine-derived actinomycetes Micromonospora sp. HS-HM-036.

    Science.gov (United States)

    Gao, Mei-Yue; Qi, Huan; Li, Jian-Song; Zhang, Hui; Zhang, Ji; Wang, Ji-Dong; Xiang, Wen-Sheng

    2017-09-01

    A new naphthalenepropanoic acid analog (1) was isolated from the broth of the actinomycetes Micromonospora sp. HS-HM-036. The structure of compound 1 was determined based on MS and extensive NMR analysis. A preliminary investigation of the biological activity of compound 1 was also described.

  9. The GATA1-HS2 enhancer allows persistent and position-independent expression of a β-globin transgene.

    Directory of Open Access Journals (Sweden)

    Annarita Miccio

    Full Text Available Gene therapy of genetic diseases requires persistent and position-independent expression of a therapeutic transgene. Transcriptional enhancers binding chromatin-remodeling and modifying complexes may play a role in shielding transgenes from repressive chromatin effects. We tested the activity of the HS2 enhancer of the GATA1 gene in protecting the expression of a β-globin minigene delivered by a lentiviral vector in hematopoietic stem/progenitor cells. Gene expression from proviruses carrying GATA1-HS2 in both LTRs was persistent and resistant to silencing at most integration sites in the in vivo progeny of human hematopoietic progenitors and murine long-term repopulating stem cells. The GATA1-HS2-modified vector allowed correction of murine β-thalassemia at low copy number without inducing clonal selection of erythroblastic progenitors. Chromatin immunoprecipitation studies showed that GATA1 and the CBP acetyltransferase bind to GATA1-HS2, significantly increasing CBP-specific histone acetylations at the LTRs and β-globin promoter. Recruitment of CBP by the LTRs thus establishes an open chromatin domain encompassing the entire provirus, and increases the therapeutic efficacy of β-globin gene transfer by reducing expression variegation and epigenetic silencing.

  10. HS-SPME/GC-MS分析发酵人参香气成分%Analysis of Volatile Components of Fermented Panax Ginseng by HS-SPME/GC-MS

    Institute of Scientific and Technical Information of China (English)

    柴贺; 周亚楠; 初琦; 王玉华; 朴春红

    2016-01-01

    研究Kefir粒发酵对人参香气成分产生的影响.鲜人参经预处理后进行Kefir粒无氧和有氧发酵,利用顶空固相微萃取与气质联用相结合的方法优化顶空萃取头类型、萃取时间、萃取温度,再分析发酵前后人参香气成分的变化.结果表明:萃取头类型为非键合型PDMS(聚二甲基硅氧烷,涂层厚度100μm)、萃取时间为30 min,萃取温度为75℃时萃取效果最佳.该条件下未发酵人参香气共50种,其中烷烃类21种,萜烯类18种,醇类4种,醛酮类2种,酚类2种,芳香类1种,含氮杂环化合物2种,人参呈土腥味;Kefir粒无氧发酵人参香气成分共64种,其中烷烃类22种,萜烯类21种,醇类9种,醛酮类4种,芳香类3种,酯类2种,酚类1种,含氮杂环化合物2种,发酵后的人参呈果香和朗姆味;Kefir粒有氧发酵人参香气成分共64种,其中烷烃19种,萜烯类22种,醇类8种,醛酮类4种,芳香类2种,酯类3种,酚酸类2种,含氮杂环化合物4种,发酵后的人参呈草药味和烧烤香味.Kefir发酵人参显著提高了人参的芳香气味.%In order to study on the effect of Kefir fermented ginseng on the aroma components of panax ginseng, headspace solid-phase microextraction (HS-SPME) and gas chromatograph coupled with mass spectrometry (GC-MS) were used for the qualitative determination of volatile components in ginseng, anaerobic fermented ginseng and aerobic fermented ginseng. The conditions of SPME fiber type, extraction time and equilibrium temperature were optimized. Optimum adsorption was achieved using PDMS (100μm) fiber for adsorption at 75 ℃for 30 min. As a result, 50 aroma compounds were extracted out of the ginseng, including 21 alkanes, 18 terpenes, 4 alcohols, 2 aldehydes and ketones, 2 phenols, 1 aromatics, 2 heterocyclic.Samples were smelled of wood and earth; 64 aroma compounds were extracted out of the anaerobic ginseng, including 22 alkanes, 21 terpenes, 9 alcohols, 4 aldehydes and ketones, 3 aromatics, 2

  11. 3-O-sulfated heparan sulfate recognized by the antibody HS4C3 contributes [corrected] to the differentiation of mouse embryonic stem cells via fas signaling.

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    Kazumi Hirano

    Full Text Available Maintenance of self-renewal and pluripotency in mouse embryonic stem cells (mESCs is regulated by the balance between several extrinsic signaling pathways. Recently, we demonstrated that heparan sulfate (HS chains play important roles in the maintenance and differentiation of mESCs by regulating extrinsic signaling. Sulfated HS structures are modified by various sulfotransferases during development. However, the significance of specific HS structures during development remains unclear. Here, we show that 3-O-sulfated HS structures synthesized by HS 3-O-sulfotransferases (3OSTs and recognized by the antibody HS4C3 increase during differentiation of mESCs. Furthermore, expression of Fas on the cell surface of the differentiated cells also increased. Overexpression of the HS4C3-binding epitope in mESCs induced apoptosis and spontaneous differentiation even in the presence of LIF and serum. These data showed that the HS4C3-binding epitope was required for differentiation of mESCs. Up-regulation of the HS4C3-binding epitope resulted in the recruitment of Fas from the cytoplasm to lipid rafts on the cell surface followed by activation of Fas signaling. Indeed, the HS4C3-binding epitope interacted with a region that included the heparin-binding domain (KLRRRVH of Fas. Reduced self-renewal capability in cells overexpressing 3OST resulted from the degradation of Nanog by activated caspase-3, which is downstream of Fas signaling, and was rescued by the inhibition of Fas signaling. We also found that knockdown of 3OST and inhibition of Fas signaling reduced the potential for differentiation into the three germ layers during embryoid body formation. This is the first demonstration that activation of Fas signaling is mediated by an increase in the HS4C3-binding epitope and indicates a novel signaling pathway for differentiation in mESCs.

  12. Gene specificity of suppression of transgene-mediated insertional transcriptional activation by the chicken HS4 insulator.

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    Romain Desprat

    Full Text Available Insertional mutagenesis has emerged as a major obstacle for gene therapy based on vectors that integrate randomly in the genome. Reducing the genotoxicity of genomic viral integration can, in first approximation, be equated with reducing the risk of oncogene activation, at least in the case of therapeutic payloads that have no known oncogenic potential, such as the globin genes. An attractive solution to the problem of oncogene activation is the inclusion of insulators/enhancer-blockers in the viral vectors. In this study we have used Recombinase-Mediated Cassette Exchange to characterize the effect of integration of globin therapeutic cassettes in the presence or absence of the chicken HS4 and three other putative insulators inserted near Stil, Tal1 and MAP17, three well-known cellular proto-oncogenes in the SCL/Tal1 locus. We show that insertion of a Locus Control Region-driven globin therapeutic globin transgene had a dramatic activating effect on Tal1 and Map17, the two closest genes, a minor effect on Stil, and no effect on Cyp4x1, a non-expressed gene. Of the four element tested, cHS4 was the only one that was able to suppress this transgene-mediated insertional transcriptional activation. cHS4 had a strong suppressive effect on the activation expression of Map17 but has little or no effect on expression of Tal1. The suppressive activity of cHS4 is therefore promoter specific. Importantly, the observed suppressive effect of cHS4 on Map17 activation did not depend on its intercalation between the LCR and the Map 17 promoter. Rather, presence of one or two copies of cHS4 anywhere within the transgene was sufficient to almost completely block the activation of Map17. Therefore, at this complex locus, suppression of transgene-mediated insertional transcriptional activation by cHS4 could not be adequately explained by models that predict that cHS4 can only suppress expression through an enhancer-blocking activity that requires intercalation

  13. Effect of Tanreqing injection on mycoplasma pneumonia in patients of hs-CRP, PCT, T cell subsets

    Institute of Scientific and Technical Information of China (English)

    Mei Bai

    2016-01-01

    Objective: To explore the effect of Tanreqing injection for treatment and its clinical significance through the changes of mycoplasma pneumonia patients with hs-CRP, PCT levels and cellular immune index. Method:A total of 114 cases of mycoplasma pneumonia, which were randomly divided into routine antibiotic treatment group (n=53) (control group), Tanreqing injection treatment group (n=61) (treatment group), to observe the two groups' hs-CRP and PCT levels in serum and the cellular immune regulation after treatment in patients, and contrast the curative effect. Results:the two groups before treatment, hs-CRP and PCT levels in serum had no significant difference;the treatment group after treatment, hs-CRP and PCT levels in serum were significantly lower than before treatment, and lower than that of the control group's;the control group after treatment of serum hs-CRP was significantly lower than that before treatment, compared to the before treatment, the level of PCT decreased slightly, but compared with before treatment there was no significant difference;after treatment, CD3+, CD4+, CD4+/CD8+, CD56+value were significantly higher than the control group's;CD8+and CD4+CD25+were significantly lower than the control group's;effective treatment group was significantly higher than the control group. The average hospitalization time was less than the control group's. Conclusion:Tanreqing injection adjuvant therapy could improve the treatment effect in mycoplasma pneumonia patients, regulate immune function, which was worth popularizing in clinical use.

  14. The Hydrogen Sulfide Donor NaHS Delays Programmed Cell Death in Barley Aleurone Layers by Acting as an Antioxidant.

    Science.gov (United States)

    Zhang, Ying-Xin; Hu, Kang-Di; Lv, Kai; Li, Yan-Hong; Hu, Lan-Ying; Zhang, Xi-Qi; Ruan, Long; Liu, Yong-Sheng; Zhang, Hua

    2015-01-01

    H2S is a signaling molecule in plants and animals. Here we investigated the effects of H2S on programmed cell death (PCD) in barley (Hordeum vulgare L.) aleurone layers. The H2S donor NaHS significantly delayed PCD in aleurone layers isolated from imbibed embryoless barley grain. NaHS at 0.25 mM effectively reduced the accumulation of superoxide anion (·O2 (-)), hydrogen peroxide (H2O2), and malondialdehyde (MDA), promoted the activity of superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and decreased those of lipoxygenase (LOX) in isolated aleurone layers. Quantitative-PCR showed that NaHS treatment of aleurone tissue led to enhanced transcript levels of the antioxidant genes HvSOD1, HvAPX, HvCAT1, and HvCAT2 and repressed transcript levels of HvLOX (lipoxygenase gene) and of two cysteine protease genes HvEPA and HvCP3-31. NaHS treatment in gibberellic acid- (GA-) treated aleurone layers also delayed the PCD process, reduced the content of ·O2 (-), and increased POD activity while decreasing LOX activity. Furthermore, α-amylase secretion in barley aleurone layers was enhanced by NaHS treatment regardless of the presence or absence of GA. These data imply that H2S acted as an antioxidant in delaying PCD and enhances α-amylase secretion regardless of the presence of GA in barley aleurone layers.

  15. The Hydrogen Sulfide Donor NaHS Delays Programmed Cell Death in Barley Aleurone Layers by Acting as an Antioxidant

    Directory of Open Access Journals (Sweden)

    Ying-Xin Zhang

    2015-01-01

    Full Text Available H2S is a signaling molecule in plants and animals. Here we investigated the effects of H2S on programmed cell death (PCD in barley (Hordeum vulgare L. aleurone layers. The H2S donor NaHS significantly delayed PCD in aleurone layers isolated from imbibed embryoless barley grain. NaHS at 0.25 mM effectively reduced the accumulation of superoxide anion (·O2-, hydrogen peroxide (H2O2, and malondialdehyde (MDA, promoted the activity of superoxide dismutase (SOD, guaiacol peroxidase (POD, catalase (CAT, and ascorbate peroxidase (APX, and decreased those of lipoxygenase (LOX in isolated aleurone layers. Quantitative-PCR showed that NaHS treatment of aleurone tissue led to enhanced transcript levels of the antioxidant genes HvSOD1, HvAPX, HvCAT1, and HvCAT2 and repressed transcript levels of HvLOX (lipoxygenase gene and of two cysteine protease genes HvEPA and HvCP3-31. NaHS treatment in gibberellic acid- (GA- treated aleurone layers also delayed the PCD process, reduced the content of ·O2-, and increased POD activity while decreasing LOX activity. Furthermore, α-amylase secretion in barley aleurone layers was enhanced by NaHS treatment regardless of the presence or absence of GA. These data imply that H2S acted as an antioxidant in delaying PCD and enhances α-amylase secretion regardless of the presence of GA in barley aleurone layers.

  16. Clinical Significance of MALB and hs-CRP in Patients with Unstable Angina Pectoris%不稳定型心绞痛患者测定UAER、hs-CRP的临床意义

    Institute of Scientific and Technical Information of China (English)

    赵有翼; 田东霞

    2012-01-01

    Objective To study the significance of urinary albumin excretion rate(UAER) and high sensitivity C-reactive protein (hs-CRP) in patients with unstable angina pectoris. Methods All 178 patients with unstable angina pectoris were divided into four groups. Initial onset angina pectoris group consisted of 46 patients, progressive angina pectoris group consisted of SO patients,spontaneous angina group consisted of 54 patients,and post-infarction angina pectoris group consisted of 28 patients. The level of UAER and hs-CRP were tested and the data were compared to that of 40 healthy controls. Results The levels of UAER and hs-CRP were higher both in initial onset angina pectoris group and progressive angina pectoris group than those of health con-trols(P <0.01). The UAER in post-infarction angina pectoris group was higher either( P < 0.05). Conclusion The level of UAER and hs-CRP in early periods of patients with unstable angina pectoris should be tested so as to get the best curing effect. Combining with myocardial zymogram and electrocardiogram examination,the proper therapy can prevent myocardial infarction.%目的 探讨尿微量白蛋白排泄率(UAER)与超敏C-反应蛋白(hs-CRP)在不稳定型心绞痛诊断中的临床意义.方法 将178例临床诊断为不稳定型心绞痛患者分为初发劳力型心绞痛组46例,恶化劳力型心绞痛组50例,自发型心绞痛组54例及梗死后心绞痛组28例;同期正常体检者40例作为对照组,分别应用免疫金标法测定尿微量白蛋白排泄率,免疫比浊法测定超敏C-反应蛋白水平,并进行比较.结果 初发型心绞痛组和恶化型心绞痛组的UAER及hs-CRP均有很大程度的增高,二者显著高于对照组(P<0.01);梗死后心绞痛组UAER明显高于对照组(P<0.05),hs-CRP在正常范围;自发型心绞痛组两项测定指标均在正常范围.结论 在心绞痛的早期应该注意监测UAER和hs-CRP的变化,以其尽早干预心绞痛的病程进展,对于

  17. Allelic Imbalance of mRNA Associated with α2-HS Glycoprotein (Fetuin-A Polymorphism

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    Yoshihiko Inaoka

    2015-01-01

    Full Text Available Alpha 2-HS glycoprotein (AHSG, also designated as fetuin-A, exhibits polymorphism in population genetics consisting of two major alleles of AHSG∗1 and AHSG∗2. The serum level in the AHSG∗1 homozygote is significantly higher than that of the AHSG∗2 homozygote. This study examined the molecular mechanism for the cis-regulatory expression. To quantitate allele-specific mRNA in intra-assays of the heterozygote, RT-PCR method employing primers that were incorporated to the two closely located SNPs was developed. The respective magnitudes of AHSG∗1 to AHSG∗2 in the liver tissues and hepatic culture cells of PLC/PRF/5 were determined quantitatively as 2.5-fold and 6.2-fold. The mRNA expressional difference of two major alleles was observed, which is consistent with that in the serum level. The culture cells carried heterozygous genotypes in rs4917 and rs4918, but homozygous one in rs2248690. It was unlikely that the imbalance was derived from the SNP located in the promotor site. Furthermore, to investigate the effect of mRNA degradation, RNA synthesis in the cell culture was inhibited potently by the addition of actinomycin-D. No marked change was apparent between the two alleles. The results indicated that the cis-regulatory expressional difference is expected to occur at the level of transcription or splicing of mRNA.

  18. Allelic Imbalance of mRNA Associated with α2-HS Glycoprotein (Fetuin-A) Polymorphism.

    Science.gov (United States)

    Inaoka, Yoshihiko; Osawa, Motoki; Mukasa, Nahoko; Miyashita, Keiko; Satoh, Fumiko; Kakimoto, Yu

    2015-01-01

    Alpha 2-HS glycoprotein (AHSG), also designated as fetuin-A, exhibits polymorphism in population genetics consisting of two major alleles of AHSG(∗) 1 and AHSG(∗) 2. The serum level in the AHSG(∗) 1 homozygote is significantly higher than that of the AHSG(∗) 2 homozygote. This study examined the molecular mechanism for the cis-regulatory expression. To quantitate allele-specific mRNA in intra-assays of the heterozygote, RT-PCR method employing primers that were incorporated to the two closely located SNPs was developed. The respective magnitudes of AHSG(∗) 1 to AHSG(∗) 2 in the liver tissues and hepatic culture cells of PLC/PRF/5 were determined quantitatively as 2.5-fold and 6.2-fold. The mRNA expressional difference of two major alleles was observed, which is consistent with that in the serum level. The culture cells carried heterozygous genotypes in rs4917 and rs4918, but homozygous one in rs2248690. It was unlikely that the imbalance was derived from the SNP located in the promotor site. Furthermore, to investigate the effect of mRNA degradation, RNA synthesis in the cell culture was inhibited potently by the addition of actinomycin-D. No marked change was apparent between the two alleles. The results indicated that the cis-regulatory expressional difference is expected to occur at the level of transcription or splicing of mRNA.

  19. Determination of benzene in different food matrices by distillation and isotope dilution HS-GC/MS

    Energy Technology Data Exchange (ETDEWEB)

    Medeiros Vinci, Raquel [Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent (Belgium); Canfyn, Michael [Food, Medicines and Consumer Safety, Scientific Institute of Public Health, Rue Juliette Wytsmanstraat 14, 1050 Brussels (Belgium); De Meulenaer, Bruno [Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent (Belgium); Schaetzen, Thibault de; Van Overmeire, Ilse; De Beer, Jacques [Food, Medicines and Consumer Safety, Scientific Institute of Public Health, Rue Juliette Wytsmanstraat 14, 1050 Brussels (Belgium); Van Loco, Joris, E-mail: Joris.VanLoco@iph.fgov.BE [Food, Medicines and Consumer Safety, Scientific Institute of Public Health, Rue Juliette Wytsmanstraat 14, 1050 Brussels (Belgium)

    2010-07-05

    Benzene is classified by the IARC as carcinogenic to humans. Several sources may contribute for the occurrence of benzene in foods, such as, environmental contamination and the reaction of benzoate salts with ascorbic acid (naturally present or added as food additives). Matrix effect on benzene recovery (e.g. in fatty foods) and artefactual benzene formation from benzoate during analysis in the presence of ascorbate are some of the challenges presented when determining benzene in a wide range of foodstuffs. Design of experiment (DOE) was used to determine the most important variables in benzene recovery from headspace GC/MS. Based on the results of the DOE, a versatile method for the extraction of benzene from all kind of food commodities was developed. The method which consisted of distillation and isotope dilution HS-GC/MS was in-house validated. Artefactual benzene was prevented by addition of a borate buffer solution (pH 11) under distillation conditions. The method presented in this study allows the use of a matrix-independent calibration with detection limits below the legal limit established by the European Council for benzene in drinking water (1 {mu}g L{sup -1}).

  20. Determination of benzene in different food matrices by distillation and isotope dilution HS-GC/MS.

    Science.gov (United States)

    Vinci, Raquel Medeiros; Canfyn, Michael; De Meulenaer, Bruno; de Schaetzen, Thibault; Van Overmeire, Ilse; De Beer, Jacques; Van Loco, Joris

    2010-07-01

    Benzene is classified by the IARC as carcinogenic to humans. Several sources may contribute for the occurrence of benzene in foods, such as, environmental contamination and the reaction of benzoate salts with ascorbic acid (naturally present or added as food additives). Matrix effect on benzene recovery (e.g. in fatty foods) and artefactual benzene formation from benzoate during analysis in the presence of ascorbate are some of the challenges presented when determining benzene in a wide range of foodstuffs. Design of experiment (DOE) was used to determine the most important variables in benzene recovery from headspace GC/MS. Based on the results of the DOE, a versatile method for the extraction of benzene from all kind of food commodities was developed. The method which consisted of distillation and isotope dilution HS-GC/MS was in-house validated. Artefactual benzene was prevented by addition of a borate buffer solution (pH 11) under distillation conditions. The method presented in this study allows the use of a matrix-independent calibration with detection limits below the legal limit established by the European Council for benzene in drinking water (1 microg L(-1)).

  1. Integrated HS and ALS Remote Sensing Data Sources to Develop Green Corridors in Sopron Mountains

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    János Tamás

    2015-01-01

    Full Text Available The green corridors are part of the European Union rural landscapes, but the regional increase in size of agricultural parcels had a significant effect on European land use in the 20th century. This effect radically reduced the coverage of natural forest. One ecological problem with the remaining forests is the partial missing of the network connecting the parts with ecological green corridors. Another economical problem is the verifiability for the payment system of agroforestry. Remote sensing methods are currently used to supervise monitoring the arable lands, plantations, natural reserve areas, as well as to help for the European Union payment system. Nowadays the airborne hyperspectral (HS and LiDAR (Light Detection And Ranging; ALS remote sensing technologies are becoming of more widespread use. They can be applied especially in spatial decision support system (SDSS, used in decisions about for nature, environment, forests, agriculture protection, conservation and monitoring, as well as for monitoring of biomass production. Our site selection model was the first step towards planning an agroforestry plantation which uses these integrated technologies to connect the parts with green corridors.

  2. Subaru high resolution spectroscopy of complex metal absorption lines of QSO HS1603+3820

    CERN Document Server

    Misawa, T; Takada-Hidai, M; Wang, Y; Kashikawa, N; Iye, M; Tanaka, I; Misawa, Toru; Yamada, Toru; Takada-Hidai, Masahide; Wang, Yiping; Kashikawa, Nobunari; Iye, Masanori; Tanaka, Ichi

    2002-01-01

    We present a high resolution spectrum of the quasar, HS1603+3820 (z_em=2.542), observed with the High Dispersion Spectrograph (HDS) on Subaru Telescope. This quasar, first discovered in the Hamburg/CfA Quasar Survey, has 11 C IV lines at 1.96 2.29 and resolves some of them into multiple narrow components with b 65 km/s). We use three properties of C IV lines, specifically, time variability, covering factor, and absorption line profile, to classify them into quasar intrinsic absorption lines (QIALs) and spatially intervening absorption lines (SIALs). The C IV lines at 2.42 < z_abs < 2.45 are classified as QIALs in spite of their large velocity shifts from the quasar. Perhaps they are produced by gas clouds ejected from the quasar with the velocity of v_ej = 8000 km/s -- 10000 km/s. On the other hand, three C IV lines at 2.48 < z_abs < 2.55 are classified as SIALs, which suggests there exist intervening absorbers near the quasar. We, however, cannot rule out QIALs for the two lines at z_abs ~ 2.54...

  3. Time-Variable Complex Metal Absorption Lines in the Quasar HS1603+3820

    CERN Document Server

    Misawa, T; Charlton, J C; Tajitsu, A; Misawa, Toru; Eracleous, Michael; Charlton, Jane C.; Tajitsu, Akito

    2005-01-01

    We present a new spectrum of the quasar HS1603+3820 taken 1.28 years (0.36 years in the quasar rest frame) after a previous observation with Subaru+HDS. The new spectrum enables us to search for time variability as an identifier of intrinsic narrow absorption lines (NALs). This quasar shows a rich complex of C IV NALs within 60,000 km/s of the emission redshift. Based on covering factor analysis, Misawa et al. found that the C IV NAL system at z_abs= 2.42--2.45 (System A, at a shift velocity of v_sh = 8,300--10,600 km/s relative to the quasar) was intrinsic to the quasar. With our new spectrum, we perform time variability analysis as well as covering factor analysis to separate intrinsic NALs from intervening NALs for 8 C IV systems. Only System A, which was identified as an intrinsic system in the earlier paper by Misawa et al., shows a strong variation in line strength (W_obs ~ 10.4A -> 19.1A). We speculate that a broad absorption line (BAL) could be forming in this quasar. We illustrate the plausibility of...

  4. Study on the Aromatic Components of Green Plum Wine by HS-SPME-GC-MS

    Institute of Scientific and Technical Information of China (English)

    Yingying MA; Hechuan WU; Qingbin LIU; Lingyun WANG; Xiao DOU; Lin YANG; Jiangang YANG

    2016-01-01

    In order to better blend green plum wine and study aromatic components of green plum wine,a qualitative analysis on aromatic components of soaked base liquor,green plum soaked wine,green plum juice,and fermented wine of green plum juice by Head Space Solid-phase Microextraction( HS-SPME) and Gas Chromatograph Mass Spectrometer( GC-MS) was studied in this paper. Experiment results indicated that14,32,17,and 46 kinds of aromatic components were identified respectively from four samples. Different aromatic components determined the special flavor and taste of green plum wine. Unique aromatic components generated in soaking process include benzaldehyde,1-butanol,2-methyl-,S-(-),benzoic acid ethyl ester,and 5-( hydroxymethyl). Special aromatic components in green plum juice were furfural,phenylethyl alcohol,and benzyl alcohol. The aromatic components in fermented wine of green plum juice mainly included phenylethyl alcohol( 6. 941%,relative content of peak area,same below),1-butanol,3-methyl-( 6. 940%),octanoic acid,ethyl ester( 3. 734%),decanoic acid,ethyl ester( 2. 590%),hexanoic acid,ethyl ester( 2. 479%),ethyl 9-decenoate( 2. 080%),and 5-hydroxymethyl( 1. 756%). This study was expected to provide scientific basis and data reference for quality improvement of green plum wine.

  5. Developing an emulsion model system containing canthaxanthin biosynthesized by Dietzia natronolimnaea HS-1.

    Science.gov (United States)

    Gharibzahedi, Seyed Mohammad Taghi; Razavi, Seyed Hadi; Mousavi, Seyed Mohammad

    2012-11-01

    An acceptable strategy to incorporate canthaxanthin (CX) as a natural colorant into products is by means of oil-in-water emulsions. The used CX in this study was produced by bacterium Dietzia natronolimnaea HS-1 using a batch bioreactor system. A central composite rotatable design-response surface methodology (CCRD-RSM) consisting of three-factored factorial design with five levels was applied for analysis of the results to obtain the optimal formulation of emulsions. Three independent variables including fenugreek gum (FG, 0.2-0.5%, w/w), coconut oil (CO, 6-10%, w/w), and CO/CX ratio (10:1-50:1) were transformed to coded values and second-order polynomial models was developed to predict the responses (pproperties such as volume-weighted mean diameter (D₄₃), specific surface area (S(v)) and polydispersity index (PDI) of emulsions. The 3-D response surface plot derived from the mathematical models was used to determine the optimal conditions. Main emulsion components under the optimum conditions ascertained presently by RSM: 50:1 CO/CX ratio, 0.49% (w/w) FG content and 6.28% (w/w) CO concentration. At this optimum point, stability, viscosity, D₄₃, S(v) and PDI were 90.6%, 0.0118 Pas, 0.595 μm, 12.03 m²/ml and 1.380, respectively.

  6. Canthaxanthin biosynthesis by Dietzia natronolimnaea HS-1: effects of inoculation and aeration rate

    Directory of Open Access Journals (Sweden)

    Forouzan Rostami

    2014-06-01

    Full Text Available The interest in production of natural colorants by microbial fermentation has been currently increased. The effects of D-glucose concentration (3.18-36.82 g/L, inoculum size (12.5 x 10(9-49.5 x 10(9 cfu cells/mL and air-flow rate (1.95-12.05 L/L min on the biomass, total carotenoid and canthaxanthin (CTX accumulation of Dietzia natronolimnaea HS-1 in a batch bioreactor was scrutinized using a response surface methodology-central composite rotatable design (RSM-CCRD. Second-order polynomial models with high R² values ranging from 0.978 to 0.990 were developed for the studied responses using multiple linear regression analysis. The models showed the maximum cumulative amounts of biomass (7.85 g/L, total carotenoid (5.48 mg/L and CTX (4.99 mg/L could be achieved at 23.38 g/L of D-glucose, 31.2 x 10(9 cfu cells/mL of inoculation intensity and air-flow rate of 7.85 L/L min. The predicted values for optimum conditions were in good agreement with experimental data.

  7. The orbital and superhump periods of the dwarf nova HS 0417+7445 in Camelopardalis

    CERN Document Server

    Shears, J H; Brady, S; Dubovsky, P; Miller, I; Staels, B

    2010-01-01

    We present the 2005-2010 outburst history of the SUUMa-type dwarf HS 0417+7445,along with a detailed analysis of extensive time-series photometry obtained in March 2008 during the second recorded superoutburst of the system. The mean outburst interval is 197 \\pm 59 d, with a median of 193 d. The March 2008 superoutburst was preceeded by a precursor outburst, had an amplitude of 4.2 magnitudes, and the whole event lasted about 16 days. No superhumps were detected during the decline from the precursor outburst, and our data suggests instead that orbital humps were present during that phase. Early superhumps detected during the rise to the superoutburst maximum exhibited an unusually large fractional period excess of epsilon = 0.137 (Psh = 0.0856(88) d). Following the maximum, a linear decline in brightness followed, lasting at least 6 days. During this decline, a stable superhump period of Psh = 0.07824(2) d was measured. Superimposed on the superhumps were orbital humps, which allowed us to accurately measure ...

  8. Production and decay of element 114: high cross sections and the new nucleus 277Hs

    Energy Technology Data Exchange (ETDEWEB)

    Dullmann, Ch.E.; Schadel, M.; Yakushev, A.; Turler, A.; Eberhardt, K.; Kratz, J.V.; Ackermann, D.; Andersson, L.-L.; Block, M.; Bruchle, W.; Dvorak, J.; Essel, H.G.; Ellison, P.A.; Even, J.; Gates, J.M.; Gorshkov, A.; Graeger, R.; Gregorich, K.E.; Hartmann, W.; Herzberg, R.-D.; Hessberger, F.P.; Hild, D.; Hubner, A.; Jager, E.; Khuyagbaatar, J.; Kindler, B.; Krier, J.; Kurz, N.; Lahiri, S.; Liebe, D.; Lommel, B.; Maiti, M.; Nitsche, H.; Omtvedt, J.P.; Parr, E.; Rudolph, D.; Runke, J.; Schausten, B.; Schimpf, E.; Semchenkov, A.; Steiner, J.; Thorle-Pospiech, P.; Uusitalo, J.; Wegrzecki, M.; Wiehl, N.

    2009-12-22

    The fusion-evaporation reaction {sup 244}Pu({sup 48}Ca,3-4n){sup 288,289}114 was studied at the new gas-filled recoil separator TASCA. Thirteen correlated decay chains were observed and assigned to the production and decay of {sup 288,289}114. At a compound nucleus excitation energy of E* = 39.8-43.9 MeV, the 4n evaporation channel cross section was 9.8{sub -3.1}{sup +3.9} pb. At E* = 36.1-39.5 MeV, that of the 3n evaporation channel was 8.0{sub -4.5}{sup +7.4} pb. In one of the 3n evaporation channel decay chains, a previously unobserved {alpha} branch in {sup 281}Ds was observed (probability to be of random origin from background: 0.1%). This {alpha} decay populated the new nucleus {sup 277}Hs, which decayed by spontaneous fission after a lifetime of 4.5 ms.

  9. Analysis of Five Earthy-Musty Odorants in Environmental Water by HS-SPME/GC-MS

    Directory of Open Access Journals (Sweden)

    Zhen Ding

    2014-01-01

    Full Text Available The pressing issue of earthy and musty odor compounds in natural waters, which can affect the organoleptic properties of drinking water, makes it a public health concern. A simple and sensitive method for simultaneous analysis of five odorants in environmental water was developed by headspace solid-phase microextraction (HS-SPME coupled to chromatography-mass spectrometry (GC-MS, including geosmin (GSM and 2-methylisoborneol (2-MIB, as well as dimethyl trisulfide (DMTS, β-cyclocitral, and β-ionone. Based on the simple modification of original magnetic stirrer purchased from CORNING (USA, the five target compounds can be separated within 23 min, and the calibration curves show good linearity with a correlation coefficient above 0.999 (levels = 5. The limits of detection (LOD are all below 1.3 ng L−1, and the relative standard deviation (%RSD is between 4.4% and 9.9% (n = 7 and recoveries of the analytes from water samples are between 86.2% and 112.3%. In addition, the storage time experiment indicated that the concentrations did not change significantly for GSM and 2-MIB if they were stored in canonical environment. In conclusion, the method in this study could be applied for monitoring these five odorants in natural waters.

  10. Stabilization of canthaxanthin produced by Dietzia natronolimnaea HS-1 with spray drying microencapsulation.

    Science.gov (United States)

    Hojjati, Mohammad; Razavi, Seyed Hadi; Rezaei, Karamatollah; Gilani, Kambiz

    2014-09-01

    The strain bacterium Dietzia natronolimnaea has propounded as a source for biological production of canthaxanthin. Because of sensitivity of this pigment, examine on its stability is important. In this study, stability of encapsulated canthaxanthin from D. natronolimnaea HS-1 using soluble soybean polysaccharide (SSPS), gum acacia (GA), and maltodextrin (MD) as wall materials was investigated at 4, 25, and 45 °C in light and dark conditions during 4 months of storage. It was shown that the type of walls influenced the size of emulsion droplets; spray dried particles, microencapsulation efficiency (ME), and retention of canthaxanthin in microcapsules. SSPS and MD produced the smallest and the biggest emulsion droplets and spray dried particles, respectively. Microcapsules made with SSPS resulted in better ME and higher stability for canthaxanthin. Samples were degraded in all conditions, especially in light and 45 °C. Degradation of microencapsulated canthaxanthin with SSPS and GA proceeded more slowly than did with MD. Regardless of the type of wall materials, total canthaxanthin contents of the microencapsulated products decreased by an increase in time or temperature. Also, samples exposed to light indicated less stability at 4 and 25 °C when compared to the storage at dark conditions. According to the results of this study, SSPS can be considered as potential wall material for the encapsulation of carotenoids.

  11. Definition of the switch surface in the solution structure of Cdc42Hs.

    Science.gov (United States)

    Feltham, J L; Dötsch, V; Raza, S; Manor, D; Cerione, R A; Sutcliffe, M J; Wagner, G; Oswald, R E

    1997-07-22

    Proteins of the rho subfamily of ras GTPases have been shown to be crucial components of pathways leading to cell growth and the establishment of cell polarity and mobility. Presented here is the solution structure of one such protein, Cdc42Hs, which provides insight into the structural basis for specificity of interactions between this protein and its effector and regulatory proteins. Standard heteronuclear NMR methods were used to assign the protein, and approximately 2100 distance and dihedral angle constraints were used to calculate a set of 20 structures using a combination of distance geometry and simulated annealing refinement. These structures show overall similarity to those of other GTP-binding proteins, with some exceptions. The regions corresponding to switch I and switch II in H-ras are disordered, and no evidence was found for an alpha-helix in switch II. The 13-residue insertion, which is only present in rho-subtype proteins and has been shown to be an important mediator of binding of regulatory and target proteins, forms a compact structure containing a short helix lying adjacent to the beta4-alpha3 loop. The insert forms one edge of a "switch surface" and, unexpectedly, does not change conformation upon activation of the protein by the exchange of GTP analogs for GDP. These studies indicate the insert region forms a stable invariant "footrest" for docking of regulatory and effector proteins.

  12. Tissue-Specific Methylation of Long Interspersed Nucleotide Element-1 of Homo Sapiens (L1Hs) During Human Embryogenesis and Roles in Neural Tube Defects.

    Science.gov (United States)

    Wang, L; Chang, S; Guan, J; Shangguan, S; Lu, X; Wang, Z; Wu, L; Zou, J; Zhao, H; Bao, Y; Qiu, Z; Niu, B; Zhang, T

    2015-01-01

    Epigenetic regulation of long interspersed nucleotide element-1 (LINE-1) retrotransposition events plays crucial roles during early development. Previously we showed that LINE-1 hypomethylation in neuronal tissues is associated with pathogenesis of neural tube defect (NTD). Herein, we further evaluated LINE-1 Homo sapiens (L1Hs) methylation in tissues derived from three germ layers of stillborn NTD fetuses, to define patterns of tissue specific methylation and site-specific hypomethylation at CpG sites within an L1Hs promoter region. Stable, tissue-specific L1Hs methylation patterns throughout three germ layer lineages of the fetus, placenta, and maternal peripheral blood were observed. Samples from maternal peripheral blood exhibited the highest level of L1Hs methylation (64.95%) and that from placenta showed the lowest (26.82%). Between samples from NTDs and controls, decrease in L1Hs methylation was only significant in NTD-affected brain tissue at 7.35%, especially in females (8.98%). L1Hs hypomethylation in NTDs was also associated with a significant increase in expression level of an L1Hs-encoded transcript in females (r = -0.846, p = 0.004). This could be due to genomic DNA instability and alternation in chromatins accessibility resulted from abnormal L1Hs hypomethylation, as showed in this study with HCT-15 cells treated with methylation inhibitor 5-Aza.

  13. Effect of soil fulvic and humic acid on binding of Pb to goethite–water interface: Linear additivity and volume fractions of HS in the Stern layer

    NARCIS (Netherlands)

    Xiong, J.; Koopal, L.K.; Weng, L.; Wang, J.; Tan, W.

    2015-01-01

    The effects of soil fulvic (JGFA) and humic acid (JGHA) on Pb binding to goethite were investigated with batch experiments and modeling. The CD-MUSIC and NICA-Donnan model could describe the Pb binding to, respectively, the binary Pb–goethite and Pb–HS systems. The adsorption of humic substances (HS

  14. 血清hs-CRP测定对糖尿病并发脑梗死患者的临床意义

    Institute of Scientific and Technical Information of China (English)

    陈干明; 王晴; 张欣中

    2012-01-01

    目的 探讨糖尿病并发急性脑梗死患者血清hs-CRP水平与病情及预后的关系.方法 测定88例糖尿病并发急性脑梗死患者血清hs-CRP含量,分析与病情及预后关系.结果 糖尿病并发急性脑梗死患者血清hs-CRP水平明显高于糖尿病并发脑腔隙性脑梗死患者(P<0.01);脑梗死重型组血清hs-CRP水平高于中型、轻型组(P<0.05);随防1年,血清hs-CRP水平高者易复发.结论 糖尿病并发急性脑梗死患者血清hs-CRP水平升高,提示患者病情重、预后差、易复发.早期测定血清hs-CRP水平有助于评估糖尿病并发急性脑梗死的病情及预后.%Objective To the correlation of the serum hs - CRP level, the clinical situation and the prognosis of the patient with diabetes complicated with acute cerebral infarction. Methods 88 patients with diabetes complicated with acute cerebral infarction were measure of their serum hs - CRP level, the correlation to the clinical situation and prognosis was ananlyzed. Results The serum hs - CRP level of the patient with diabetes complicated with acute cerebral infarction was significantly higher than that of the patient with diabetes complicated with cerebral lacunar infarction ( P < 0. 01) , the serum hs - CRP level was higher in the severe infarction than in the medium and mild group ( P < 0. 05) , the patients were followed up for one year and those with high level of serum hs - CRP was more easy for relatpsing. Conclusions The serum hs - CRP level in the patient with diabetes complicated with acute cerebral infarction is elevated, which indicates a more severe clinical situation and poor prognosis. The early measurement of serum hs - CRP helps for the evaluation of diabetes complicated with acute cerebral infarction and its prognosis.

  15. Resting Serum Concentration of High-Sensitivity C-Reactive Protein (hs-CRP) in Sportsmen and Untrained Male Adults.

    Science.gov (United States)

    Niyi-Odumosu, F A; Bello, O A; Biliaminu, S A; Owoyele, B V; Abu, T O; Dominic, O L

    2017-03-06

    There is an inverse relationship between regular physical activity and concentration of serum inflammatory markers, with variations in resting CRP in trained and untrained subjects. The effect of acute and prolonged exercises has been studied on inflammatory markers with dearth of information and controversies on the resting serum values of high sensitivity CRP (hs-CRP). Therefore, this study sought to identify and compare variations that occur in serum levels of high sensitivity CRP in groups of sportsmen (6) and physically active untrained subjects. Eighty-one healthy male participants made up of 21 untrained (control), 10 footballers, 10 athletes, 10 karates, 10 volleyballers, 10 basketballers, and 10 baseballers voluntarily participated in the study. Participants rested while in sitting position for about 30 minutes during which blood pressures and heart rates were taken. 5 mls of venous blood was withdrawn from the antecubital vein of the participants (aseptically) between 7:00 and 10:00 am into lithium heparin bottles following an overnight fast. The supernatant was decanted and centrifuged at 3000 rpm, serum was collected and stored at -20ºC prior to biochemical assay which was done with the use of enzyme linked immunosorbent assay (ELISA) kits for hs-CRP. Differences in the means within the sporting groups were analysed using one-way ANOVA while the difference between the trained sportsmen and untrained young adults was analysed using the independent T-test. Statistical significance was set at p hs-CRP (µg/ml) was 1.0±0.2 in the untrained, 2.6±0.7 in footballers, 3.6±2.1 in track athletes, 2.4±0.5 in basketballers, 2.2±0.5 in volleyballers, 2.4±1.3 in baseballers, and 1.7±0.5 in karate respectively. There was no significant difference in the resting hs-CRP amongst the sporting groups, and in the sportsmen and untrained group. Resting serum levels of hs-CRP falls within normal range, and varies insignificantly in groups of sports, and in sportsmen

  16. Advances in understanding of soil biogeochemical cycles: the mechanism of HS entry into the root interior

    Science.gov (United States)

    Aleksandrova, Olga

    2017-04-01

    Humic substances represent the major reservoir of carbon (C) in ecosystems, and their turnover is crucial for understanding the global C cycle. As shown by some investigators [1-2], the phenomenon of the uptake of the whole humic particles by plant roots is a significant step of biogeochemical cycle of carbon in soils. The mechanism of HS entry the root interior remained unknown for a long time. However recently, the last one was discovered [3]. An advanced model [3] includes two hypotheses. These hypotheses are as follows: (1) each nano-size particle possesses a quantum image that can be revealed as a packet of electromagnetic waves; (2) the interaction of nano-size particle with the membrane (plasma membrane) of living cells, on which it is adsorbed, occurs via the development of the Rayleigh-Taylor (RT) instability on the membrane surface. An advanced model allows us to look insight some into some phenomena that were observed by experiments but remained not understood [2]. The authors [2] applied tritium autoradiography to wheat seedlings cultivated with tritium-labeled HS to consider the uptake of humic particles by plant roots. They found a significant increase in the content of some polar (monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulfoquinovosyl diacylglycerol (SQDG) and phosphatidylcholine (PC)) and neutral (free fatty acids, FFA) lipids which were detected in the wheat seedlings treated with humic particles. Authors [2] pointed that lipids MGDG, DGDG, SQDG are crucial for functional and structural integrity of the photosystem complex. Therefore, a stimulating action of adsorbed humic particles evoked phenomena like photosynthesis in root cells that can be interpreted using an advanced model: humic particles being nano-size particles become adsorbed on the plant roots in soils, and influence their micro environment, where they are located, with the specific electromagnetic exposure. Another finding of authors consisted in the

  17. Urban Transformation and Property Value Variation. The Role of HS Stations

    Directory of Open Access Journals (Sweden)

    Carmela Gargiulo

    2010-04-01

    operation, partly in operation or not yet in operation. Besides, the description of each case is divided into: summary description of the characteristics of the HS railway line; description of the main features of cities that represent the nodes of the selected routes; identify the characteristics of the surrounding context of the railway station; measurement of the change in property values in the surrounding context of the railway station due to HS link. The third part provides a comparative outline of the effects of High Speed stations on property values and the urban typologies and characteristics that influence this relation. The first result of this article is a comparative framework between all study cases. In conclusion, the comparative reading shows that the effect from opening of the High Speed railway stations on property values is valuable in cases where it contributes significantly to raising the socio-economic and the quality conditions of spaces.

  18. Theses\\' subjects chosen from public needs: Experience of Yazd Health Study (YaHS

    Directory of Open Access Journals (Sweden)

    AA Dehghanitaftiti

    2014-12-01

    Full Text Available Yazd Health Study (YaHS is an endeavor to involve students in design, implementation and analysis of a large study. Over 150 graduate and post graduate students from various disciplines have been invited to participate in a large longitudinal study with 10000 samples. Participants were selected randomly from Yazd population, over 1000000 as December 2014. Four hundred variables grouped in the following sections were asked 1 demographics, 2 physical activity, 3sleep quality and quantity, 4 mental health, 5 history y of chronic illnesses, 6 history of surgical operations, 7 dental health, 8 history of accidents, 9 dietetic habits, 10 occupation and communication history, 11 traditional medicine, 12 history of smoking and addiction and 13 women’s health. The interview follows by anthropometric measurements including weight, height, BMI, waist circumference, hip circumference, neck circumference and WHR. Per cent body fat, % body muscle, visceral fat, resting metabolism have been measured followed by pulse and blood pressure. Students were trained to participate in design, piloting and the main phase of data collection. Students evaluated the experience useful and constructive. In return of their collaboration in interviewing 50-100 study participants, they will receive data for their theses' proposal. This will help them to access a large and high quality set of data and disseminate the results in high quality peer reviewed journals as well as national and international conferences. The students mandated to write an executive summary of their thesis and disseminate it to health managers and inter-sectoral decision makers. They will gain bonus score if they can publish their main findings in public media. Acknowledgments: Thanks to students who participated in the study and A. Professor Masoud Mirzaei, the principal investigator of Yazd Health Study (YAHS for providing information on the design and the progress of the study.

  19. Laminin peptide YIGSR induces collagen synthesis in Hs27 human dermal fibroblasts

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Jong Hyuk; Kim, Jaeyoon; Lee, Hyeongjoo [NovaCell Technology Inc., Pohang, Kyungbuk 790-784 (Korea, Republic of); Kim, So Young [Department of Dermatology, Chung-Ang University College of Medicine, Seoul 156-756 (Korea, Republic of); Department of Convergence Medicine and Pharmaceutical Biosciences, Graduate School, Chung-Ang University, Seoul 156-756 (Korea, Republic of); Jang, Hwan-Hee [Functional Food and Nutrition Division, Department of Agrofood Resources, Rural Development Administration, Suwon 441-853 (Korea, Republic of); Ryu, Sung Ho [Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang, Kyungbuk 790-784 (Korea, Republic of); Kim, Beom Joon [Department of Dermatology, Chung-Ang University College of Medicine, Seoul 156-756 (Korea, Republic of); Department of Convergence Medicine and Pharmaceutical Biosciences, Graduate School, Chung-Ang University, Seoul 156-756 (Korea, Republic of); Lee, Taehoon G., E-mail: taehoon@novacelltech.com [NovaCell Technology Inc., Pohang, Kyungbuk 790-784 (Korea, Republic of)

    2012-11-23

    Highlights: Black-Right-Pointing-Pointer We identify a function of the YIGSR peptide to enhance collagen synthesis in Hs27. Black-Right-Pointing-Pointer YIGSR peptide enhanced collagen type 1 synthesis both of gene and protein levels. Black-Right-Pointing-Pointer There were no changes in cell proliferation and MMP-1 level in YIGSR treatment. Black-Right-Pointing-Pointer The YIGSR effect on collagen synthesis mediated activation of FAK, pyk2 and ERK. Black-Right-Pointing-Pointer The YIGSR-induced FAK and ERK activation was modulated by FAK and MEK inhibitors. -- Abstract: The dermal ECM is synthesized from fibroblasts and is primarily compromised of fibrillar collagen and elastic fibers, which support the mechanical strength and resiliency of skin, respectively. Laminin, a major glycoprotein located in the basement membrane, promotes cell adhesion, cell growth, differentiation, and migration. The laminin tyrosine-isoleucine-glycine-serine-arginine (YIGSR) peptide, corresponding to the 929-933 sequence of the {beta}1 chain, is known to be a functional motif with effects on the inhibition of tumor metastasis, the regulation of sensory axonal response and the inhibition of angiogenesis through high affinity to the 67 kDa laminin receptor. In this study, we identified a novel function of the YIGSR peptide to enhance collagen synthesis in human dermal fibroblasts. To elucidate this novel function regarding collagen synthesis, we treated human dermal fibroblasts with YIGSR peptide in both a time- and dose-dependent manner. According to subsequent experiments, we found that the YIGSR peptide strongly enhanced collagen type 1 synthesis without changing cell proliferation or cellular MMP-1 level. This YIGSR peptide-mediated collagen type 1 synthesis was modulated by FAK inhibitor and MEK inhibitor. This study clearly reveals that YIGSR peptide plays a novel function on the collagen type 1 synthesis of dermal fibroblasts and also suggests that YIGSR is a strong candidate

  20. Quantification of Polyfunctional Thiols in Wine by HS-SPME-GC-MS Following Extractive Alkylation

    Directory of Open Access Journals (Sweden)

    Lauren E. Musumeci

    2015-07-01

    Full Text Available Analyses of key odorous polyfunctional volatile thiols in wines (3-mercaptohexanol (3-MH, 3-mercaptohexylacetate (3-MHA, and 4-mercapto-4-methyl-2-pentanone (4-MMP are challenging due to their high reactivity and ultra-trace concentrations, especially when using conventional gas-chromatography electron impact mass spectrometry (GC-EI-MS. We describe a method in which thiols are converted to pentafluorobenzyl (PFB derivatives by extractive alkylation and the organic layer is evaporated prior to headspace solid phase microextraction (HS-SPME and GC-EI-MS analysis. Optimal parameters were determined by response surface area modeling. The addition of NaCl solution to the dried SPME vials prior to extraction resulted in up to less than fivefold improvement in detection limits. Using 40 mL wine samples, limits of detection for 4-MMP, 3-MH, and 3-MHA were 0.9 ng/L, 1 ng/L, and 17 ng/L, respectively. Good recovery (90%–109% and precision (5%–11% RSD were achieved in wine matrices. The new method was used to survey polyfunctional thiol concentrations in 61 commercial California and New York State wines produced from V. vinifera (Riesling, Gewürztraminer, Cabernet Sauvignon, Sauvignon blanc and non-varietal rosé wines, V. labruscana (Niagara, and Vitis spp. (Cayuga White. Mean 4-MMP concentrations in New York Niagara (17 ng/L were not significantly different from concentrations in Sauvignon blanc, but were significantly higher than 4-MMP in other varietal wines.

  1. Online breath gas analysis in unrestrained mice by hs-PTR-MS.

    Science.gov (United States)

    Szymczak, Wilfried; Rozman, Jan; Höllriegl, Vera; Kistler, Martin; Keller, Stefan; Peters, Dominika; Kneipp, Moritz; Schulz, Holger; Hoeschen, Christoph; Klingenspor, Martin; de Angelis, Martin Hrabě

    2014-04-01

    The phenotyping of genetic mouse models for human disorders may greatly benefit from breath gas analysis as a noninvasive tool to identify metabolic alterations in mice. Phenotyping screens such as the German Mouse Clinic demand investigations in unrestrained mice. Therefore, we adapted a breath screen in which exhaled volatile organic compounds (VOCs) were online monitored by proton transfer reaction mass spectrometry (hs-PTR-MS). The source strength of VOCs was derived from the dynamics in the accumulation profile of exhaled VOCs of a single mouse in a respirometry chamber. A careful survey of the accumulation revealed alterations in the source strength due to confounders, e.g., urine and feces. Moreover changes in the source strength of humidity were triggered by changes in locomotor behavior as mice showed a typical behavioral pattern from activity to settling down in the course of subsequent accumulation profiles. We demonstrated that metabolic changes caused by a dietary intervention, e.g., after feeding a high-fat diet (HFD) a sample of 14 male mice, still resulted in a statistically significant shift in the source strength of exhaled VOCs. Applying a normalization which was derived from the distribution of the source strength of humidity and accounted for varying locomotor behaviors improved the shift. Hence, breath gas analysis may provide a noninvasive, fast access to monitor the metabolic adaptation of a mouse to alterations in energy balance due to overfeeding or fasting and dietary macronutrient composition as well as a high potential for systemic phenotyping of mouse mutants, intervention studies, and drug testing in mice.

  2. 中度嗜盐菌HS1产纤维素酶发酵条件的研究%Study on Cellulase Production by A Moderately Halophilic Bacterium HS1

    Institute of Scientific and Technical Information of China (English)

    韩秋菊; 张倩倩

    2011-01-01

    对一株中度嗜盐菌HS1产纤维素酶的活性进行了初步研究.单因素实验和正交实验结果表明,菌株HS1产纤维素酶的最佳发酵氮源为1.5%NaNO3,优化的发酵条件为:液体种子接种量6%、培养温度35℃、初始pH值6.5、培养时间4 d.%In this paper, cellulase production by a moderately halophilic bacterium HS1 was preliminarily explored. Through single factor experiment and orthogonal experiment, it was found that the optimal nitrogen source was 1.5% NaNO3 for production of cellulase and the optimum fermentation conditions were as follows:inoculating quantity of liquid seed of 6% ,culture temperature of 35℃, initial pH value of 6.5 and culture time of 4 d.

  3. Optimization of HS-GC-FID-MS Method for Residual Solvent Profiling in Active Pharmaceutical Ingredients Using DoE.

    Science.gov (United States)

    Poceva Panovska, Ana; Acevska, Jelena; Stefkov, Gjoshe; Brezovska, Katerina; Petkovska, Rumenka; Dimitrovska, Aneta

    2016-02-01

    Within this research, a headspace (HS) gas chromatography-flame ionization detector-mass spectrometry method was developed for profiling of residual solvents (RSs) in active pharmaceutical ingredients (APIs). Design of experiment was used for optimization of sample preparation, as well as for robustness testing of the method. HS equilibration temperature and dilution medium were detected as parameters with greater impact on the sensitivity, compared with the time used for equilibration of the samples. Regardless of the sample solubility, the use of water for sample preparation was found to be crucial for better sensitivity. The use of a well-designed strategy for method development and robustness testing, additional level of identification confidence, as well as use of internal standard provided a strong and reliable analytical tool for API fingerprinting, thus enabling the authentication of the substance based on the RS profile.

  4. On the Highest Oxidation States of Metal Elements in MO4 Molecules (M = Fe, Ru, Os, Hs, Sm, and Pu).

    Science.gov (United States)

    Huang, Wei; Xu, Wen-Hua; Schwarz, W H E; Li, Jun

    2016-05-02

    Metal tetraoxygen molecules (MO4, M = Fe, Ru, Os, Hs, Sm, Pu) of all metal atoms M with eight valence electrons are theoretically studied using density functional and correlated wave function approaches. The heavier d-block elements Ru, Os, Hs are confirmed to form stable tetraoxides of Td symmetry in (1)A1 electronic states with empty metal d(0) valence shell and closed-shell O(2-) ligands, while the 3d-, 4f-, and 5f-elements Fe, Sm, and Pu prefer partial occupation of their valence shells and peroxide or superoxide ligands at lower symmetry structures with various spin couplings. The different geometric and electronic structures and chemical bonding types of the six iso-stoichiometric species are explained in terms of atomic orbital energies and orbital radii. The variations found here contribute to our general understanding of the periodic trends of oxidation states across the periodic table.

  5. 25(OHD was Correlated with Increased Risk of Insulin Resistance, but Not Mediated by Adiponectin and hsCRP

    Directory of Open Access Journals (Sweden)

    Anggi Kartikawati

    2012-08-01

    Full Text Available BACKGROUND: Studies have shown that change of calcium and vitamin D homeostasis is associated with insulin resistance, decreased beta cell function, metabolic syndrome, glucose intolerance and diabetes. Evidence suggests that vitamin D insufficiency is inversely related to risk of metabolic disorders including type-2 Diabetes Mellitus (T2DM, although the underlying mechanisms are not yet understood. Hence, current study was conducted to investigate correlation between 25(OHD and insulin resistance through adiponectin or High Sensitivity C-Reactive Protein (hsCRP in centrally obese men. METHODS: This was a cross-sectional study involving 80 centrally obese men with waist circumference (WC >90 cm and age 30-60 years. Total 25(OHD concentration was measured by Enzyme-Linked Immunosorbent Assay (ELISA method. Insulin resistance was calculated by HOMA model. RESULTS: This study showed there was no correlation of 25(OHD-WC (r=0.006 and p=0.957, 25(OHD-adiponectin (r=0.179 and p=0.111 abd 25(OHD-hsCRP (r=-0.223 and p=0.334, but we observed  statistically significant negative correlation between 25(OHD and insulin resistance index (HOMA-IR (r=0.461 and p=0.041. CONCLUSIONS: We conclude that low 25(OHD concentration was significantly associated with increased risk of insulin resistance. Since the adiponectin or hsCRP was not correlated, the possible pathways need to be futher investigated. KEYWORDS: central obesity, 25(OHD, adiponectin, hsCRP, insulin resistance (HOMA-IR.

  6. Investigating the Effect of Inflammation on Atrial Fibrillation Occurrence by Measuring Highly Sensitive C-reactive Protein (hs-CRP

    Directory of Open Access Journals (Sweden)

    Mahdi Hassanzadeh Delui

    2014-05-01

    Full Text Available Introduction: Atrial fibrillation (AF is the most prevalent cardiac arrhythmias that cardiologists and internists encounter. The goal of this article is to clarify an overview of the evidence linking inflammation to AF existence, which may highlight the effect of some pharmacological agents that have genuine potential to reduce the clinical burden of AF by modulating inflammatory pathways. Materials and Methods: In a case-control study, 50 patients with atrial fibrillation (AF with different etiologies and 50 patients with sinus rhythm and similar bases were selected. Sampling for highly sensitive c-reactive (hs-CRP was done on the patients presenting with AF to the Ghaem hospital between October 2006 and June 2007. Results: Mean age of the patients was 62 years with maximum of 90 and minimum of 36 and standard deviation of 13.80. The most frequent age group was 71-80years. Fifty-four percent of patients were male and 46% were female. Mean serum hs-CRP levels in AF patients with hypertension (HTN ,Ischemic heart disease(IHD, Valvular heart disease (VHD, HTN+IHD and hyperthyroidism were 8.10, 9.40, 8.68, 10.16 and 5.98 mg/Lit; respectively. There was significant difference between hs-CRP levels in hypertensive patients in the two groups (P=0.010. Similar results were observed in IHD patients, VHD patients and HTN+IHD patients in two groups (P=0.015, P=0.037, P=0.000. Conclusion: In addition to some risk factors like baseline cardiac diseases, aging, thyrotoxicosis, pulmonary embolism, pneumonia and cardiac surgery, there also appears to be consistent links between hs-CRP, a marker of inflammation, and the pathogenesis of AF.

  7. Correlation between the severity of coronary artery lesions and levels of estrogen, hs-CRP and MMP-9.

    Science.gov (United States)

    Guo, Changlei; Zhang, Shaoli; Zhang, Junbiao; Liu, Hui; Li, Peicheng; Liu, Hengdao; Wang, Yakun

    2014-05-01

    The aim of this study was to investigate the correlation between the severity of coronary artery lesions in patients with acute coronary syndromes (ACS) and levels of estrogen, high-sensitivity C-reactive protein (hs-CRP) and matrix metalloproteinase-9 (MMP-9). A total of 65 patients with ACS, 33 patients with stable angina pectoris (SAP) and 36 healthy controls were randomly enrolled. Patients with ACS were subdivided into two groups: Acute myocardial infarction (AMI; n=30) and unstable angina pectoris (UAP; n=35). Serum levels of estrogen, hs-CRP and MMP-9 were detected in the four groups of subjects. Serum estrogen levels in patients with AMI, UAP and SAP were significantly lower than those in the control group (Phs-CRP and MMP-9, followed in descending order by those with UAP and SAP (Phs-CRP and MMP-9 were also significantly different among the AMI, UAP and SAP groups (Phs-CRP and MMP-9 levels (r=-0.6634 and -0.6878, respectively; both Phs-CRP and MMP-9 levels correlated positively (r=0.7208, Phs-CRP and MMP-9 levels (r=0.6519 and 0.6835, respectively; both Phs-CRP and MMP-9 levels were significantly correlated with the severity of coronary artery lesions. There was also a significant correlation between serum estrogen, hs-CRP and MMP-9 levels. These data indicate that serum estrogen, hs-CRP and MMP-9 have the potential to be used as biomarkers for evaluating the severity of coronary artery lesions and the stability of coronary artery plaques.

  8. Determination of volatile aroma compounds of Ganoderma lucidum by gas chromatography mass spectrometry (HS-GC/MS).

    Science.gov (United States)

    Taşkın, Hatıra; Kafkas, Ebru; Çakıroğlu, Özgün; Büyükalaca, Saadet

    2013-01-01

    This study was conducted at Horticulture Department of Cukurova University, Adana, Turkey during 2010-2011. Fresh sample of Ganoderma lucidum collected from Mersin province of Turkey was used as material. Volatile aroma compounds were performed by Headspace Gas Chromatography (HS-GC/MS). Alcohols, aldehydes, acids, phenol, L-Alanine, d-Alanine, 3Methyl, 2-Butanamine, 2-Propanamine were determined. 1-Octen-3-ol (Alcohol) and 3-methyl butanal (Aldehyde) were identified as major aroma compounds.

  9. Non alcoholic fatty liver disease and its relationship with hsCRP in type 2 diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Naina Bhuyan

    2014-08-01

    Conclusions: High hsCRP is a cheap, easily available laboratory marker to suspect fatty liver and possibly steatohepatitis in T2 Diabetics in our region. It can identify a subgroup of diabetic patients in whom liver biopsy may be advisable to confirm steatohepatitis which is important for prognosis and therefore need aggressive intervention. [Int J Res Med Sci 2014; 2(4.000: 1586-1590

  10. The refinement of the surface layer of HS 7425 high speed tool steel by laser and electric arc plasma

    Directory of Open Access Journals (Sweden)

    W. Bochnowski

    2008-10-01

    Full Text Available The paper present two different techniques: laser remelting surface and plasma remelting surface of the high speed steel HS 7425. Thestructure of the remelted layers were examined by means of SEM – microscopy. Measurement of microhardness in remelting zone usingVickers method. The remelting zone consist of dendritic cells and columnar crystals. Increase of hardness was observed in remelted zonein comparison to the substrate of the steel. The hardness in the remelted zone increases with the increasing cooling rate.

  11. DIAGNOSTIC VALUE OF SIMULTANEOUS MEASUREMENT OF PROCALCITONIN, INTERLEUKIN-6 AND HS CRP IN PREDICTION OF EARLY-ONSET NEONATAL SEPSIS

    Directory of Open Access Journals (Sweden)

    alireza abdollahi

    2012-05-01

    Full Text Available Neonatal sepsis is a major cause of morbidities and mortalities mostly remarkable in the third world nations .We aimed to assess the value of simultaneous measurement of procalcitonin (PCT and interleukin-6 (IL-6 in association with high sensitive- C reactive protein in prediction of early neonatal sepsis. We performed a follow- up study on 95 neonates who were below 12 hours (h of age, had clinical signs of sepsis or maternal risk factors for sepsis. Neonates were assigned to 4 groups including “proven early-onset sepsis”, “clinical early-onset sepsis”, “negative infectious status”, and “uncertain infectious status”. Blood samples were obtained within the first 12 h of birth repeated between 24 hours and 36 hours of age for determination of serum levels of PCT, IL-6, high sensitivie- C Reactive Protein (hs-CRP, and white blood cell (WBC count. On admission, neonates with sepsis had a higher WBC count, IL-6, PCT, and hs-CRP levels compared with those neonates without sepsis. This remained significant even after 12-24 hours of admission. Also, patients with clinical evidences of sepsis had a higher serum level of PCT and IL-6 within 12-24 hours after admission compared to the patients with uncertain sepsis. In final The combination of IL-6, hs-CRP, and PCT seems to be predictive in diagnosis of early onset neonatal sepsis.

  12. Scoring correction for MMPI-2 Hs scale with patients experiencing a traumatic brain injury: a test of measurement invariance.

    Science.gov (United States)

    Alkemade, Nathan; Bowden, Stephen C; Salzman, Louis

    2015-02-01

    It has been suggested that MMPI-2 scoring requires removal of some items when assessing patients after a traumatic brain injury (TBI). Gass (1991. MMPI-2 interpretation and closed head injury: A correction factor. Psychological assessment, 3, 27-31) proposed a correction procedure in line with the hypothesis that MMPI-2 endorsement may be affected by symptoms of TBI. This study assessed the validity of the Gass correction procedure. A sample of patients with a TBI (n = 242), and a random subset of the MMPI-2 normative sample (n = 1,786). The correction procedure implies a failure of measurement invariance across populations. This study examined measurement invariance of one of the MMPI-2 scales (Hs) that includes TBI correction items. A four-factor model of the MMPI-2 Hs items was defined. The factor model was found to meet the criteria for partial measurement invariance. Analysis of the change in sensitivity and specificity values implied by partial measurement invariance failed to indicate significant practical impact of partial invariance. Overall, the results support continued use of all Hs items to assess psychological well-being in patients with TBI. © The Author 2014. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  13. GmHs1-1, encoding a calcineurin-like protein, controls hard-seededness in soybean.

    Science.gov (United States)

    Sun, Lianjun; Miao, Zhenyan; Cai, Chunmei; Zhang, Dajian; Zhao, Meixia; Wu, Yanyan; Zhang, Xueling; Swarm, Stephen A; Zhou, Liwen; Zhang, Zhanyuan J; Nelson, Randall L; Ma, Jianxin

    2015-08-01

    Loss of seed-coat impermeability was essential in the domestication of many leguminous crops to promote the production of their highly nutritious seeds. Here we show that seed-coat impermeability in wild soybean is controlled by a single gene, GmHs1-1, which encodes a calcineurin-like metallophosphoesterase transmembrane protein. GmHs1-1 is primarily expressed in the Malpighian layer of the seed coat and is associated with calcium content. The transition from impermeability to permeability in domesticated soybean was caused by artificial selection of a point mutation in GmHs1-1. Interestingly, a number of soybean landraces evaded selection for permeability because of an alternative selection for seed-coat cracking that also enables seed imbibition. Despite the single origin of the mutant allele Gmhs1-1, the distribution pattern of allelic variants in the context of soybean population structure and the detected signature of genomic introgression between wild and cultivated soybeans suggest that Gmhs1-1 may have experienced reselection for seed-coat permeability.

  14. The cHS4 insulator increases the probability of retroviral expression at random chromosomal integration sites.

    Science.gov (United States)

    Rivella, S; Callegari, J A; May, C; Tan, C W; Sadelain, M

    2000-05-01

    Retroviruses are highly susceptible to transcriptional silencing and position effects imparted by chromosomal sequences at their integration site. These phenomena hamper the use of recombinant retroviruses as stable gene delivery vectors. As insulators are able to block promoter-enhancer interactions and reduce position effects in some transgenic animals, we examined the effect of an insulator on the expression and structure of randomly integrated recombinant retroviruses. We used the cHS4 element, an insulator from the chicken beta-like globin gene cluster, which has been shown to reduce position effects in transgenic Drosophila. A large panel of mouse erythroleukemia cells that bear a single copy of integrated recombinant retroviruses was generated without using drug selection. We show that the cHS4 increases the probability that integrated proviruses will express and dramatically decreases the level of de novo methylation of the 5' long terminal repeat. These findings support a primary role of methylation in the silencing of retroviruses and suggest that cHS4 could be useful in gene therapy applications to overcome silencing of retroviral vectors.

  15. Sequence change in the HS2-LCR and Gg-globin gene promoter region of sickle cell anemia patients

    Directory of Open Access Journals (Sweden)

    E.V. Adorno

    2008-02-01

    Full Text Available The fetal hemoglobin (HbF levels and ßS-globin gene haplotypes of 125 sickle cell anemia patients from Brazil were investigated. We sequenced the Gg- and Ag-globin gene promoters and the DNase I-2 hypersensitive sites in the locus control regions (HS2-LCR of patients with HbF level disparities as compared to their ßS haplotypes. Sixty-four (51.2% patients had CAR/Ben genotype; 36 (28.8% Ben/Ben; 18 (14.4% CAR/CAR; 2 (1.6% CAR/Atypical; 2 (1.6% Ben/Cam; 1 (0.8% CAR/Cam; 1 (0.8% CAR/Arab-Indian, and 1 (0.8% Sen/Atypical. The HS2-LCR sequence analyses demonstrated a c.-10.677G>A change in patients with the Ben haplotype and high HbF levels. The Gg gene promoter sequence analyses showed a c.-157T>C substitution shared by all patients, and a c.-222_-225del related to the Cam haplotype. These results identify new polymorphisms in the HS2-LCR and Gg-globin gene promoter. Further studies are required to determine the correlation between HbF synthesis and the clinical profile of sickle cell anemia patients.

  16. Analysis of the optical emission of the young precataclysmic variables HS 1857+5144 and ABELL 65

    Science.gov (United States)

    Shimansky, V. V.; Pozdnyakova, S. A.; Borisov, N. V.; Bikmaev, I. F.; Vlasyuk, V. V.; Spiridonova, O. I.; Galeev, A. I.; Mel'Nikov, S. S.

    2009-10-01

    We analyze the physical state and the properties of the close binary systems HS 1857+5144 and Abell 65. We took the spectra of both systems over a wide range of orbital phases with the 6-m telescope of the Special Astrophysical Observatory of the Russian Academy of Sciences (SAO RAS) and obtained their multicolor light curves with the RTT150 and Zeiss-1000 telescopes of the SAO RAS. We demonstrate that both Abell 65 and HS 1857+5144 are young precataclysmic variables (PV) with orbital periods of P orb = 1. d 003729 and P orb = 0. d 26633331, respectively. The observed brightness and spectral variations during the orbital period are due to the radiation of the cold component, which absorbs the short-wave radiation of the hot component and reemits it in the visual part of the spectrum. A joint analysis of the brightness and radial velocity curves allowed us to find the possible and optimum sets of their fundamental parameters. We found the luminosity excesses of the secondary components of HS 1857+5144 and Abell 65 with respect to the corresponding Main Sequence stars to be typical for such objects. The excess luminosities of the secondary components of all young PVs are indicative of their faster relaxation rate towards the quiescent state compared to the rates estimated in earlier studies.

  17. HS3D, A Dataset of Homo Sapiens Splice Regions, and its Extraction Procedure from a Major Public Database

    Science.gov (United States)

    Pollastro, Pasquale; Rampone, Salvatore

    The aim of this work is to describe a cleaning procedure of GenBank data, producing material to train and to assess the prediction accuracy of computational approaches for gene characterization. A procedure (GenBank2HS3D) has been defined, producing a dataset (HS3D - Homo Sapiens Splice Sites Dataset) of Homo Sapiens Splice regions extracted from GenBank (Rel.123 at this time). It selects, from the complete GenBank Primate Division, entries of Human Nuclear DNA according with several assessed criteria; then it extracts exons and introns from these entries (actually 4523 + 3802). Donor and acceptor sites are then extracted as windows of 140 nucleotides around each splice site (3799 + 3799). After discarding windows not including canonical GT-AG junctions (65 + 74), including insufficient data (not enough material for a 140 nucleotide window) (686 + 589), including not AGCT bases (29 + 30), and redundant (218 + 226), the remaining windows (2796 + 2880) are reported in the dataset. Finally, windows of false splice sites are selected by searching canonical GT-AG pairs in not splicing positions (271 937 + 332 296). The false sites in a range +/- 60 from a true splice site are marked as proximal. HS3D, release 1.2 at this time, is available at the Web server of the University of Sannio: http://www.sci.unisannio.it/docenti/rampone/.

  18. 急性脑梗死患者血清MMP-2、MMP-9和hs-CRP检测的临床意义

    Institute of Scientific and Technical Information of China (English)

    王清峰

    2010-01-01

    目的 检测急性脑梗死(ACI)患者血清中基质金属蛋白酶-2(MMP-2)、MMP-9和超敏C反应蛋白(hs-CRP)的表达,探讨其临床意义.方法 选取102例ACI患者作为观察组,40例健康查体者作为对照组,检测两组血清MMP-2、MMP-9和hs-CRP水平.结果 观察组MMP-2、MMP-9和hs-CRP表达显著高于对照组,且随梗死范围的增大而升高(P<0.05);观察组MMP-9与hs-CRP的表达呈正相关(r=0.402,P<0.05). 结论 MMP-2、MMP-9和hs-CRP在ACI的发生发展中可能起重要作用;MMP-9和hs-CRP可能具有协同作用,早期联合检测MMP-2、MMP-9和hs-CRP有助于判断脑梗死病变程度.

  19. IMA、BNP和hsCRP在急性冠状动脉综合征早期诊断中的应用%Application of IMA,BNP and hsCRP in the diagnosis of early acute coronary syndromes

    Institute of Scientific and Technical Information of China (English)

    李宁; 刘丰海; 郭庆波; 陈增生; 杨元铭; 臧葳

    2015-01-01

    目的:探讨缺血修饰白蛋白(IMA)、B型尿钠肽(BNP)、超敏C反应蛋白(hsCRP)在急性冠脉综合征(ACS)早期诊断中的作用。方法来自青岛市立医院96例ACS患者作为治疗组,其中急性心肌梗死(AMI)42例,不稳定型心绞痛(UAP)54例,分别于胸痛发作2、4、6、12、18及24 h抽血,另选50名健康体检者作为对照组,两组均检测其IMA、BNP、hsCRP含量,评价这三项指标在ACS早期的应用价值。结果在急性冠状动脉综合征患者中IMA水平于胸痛发作2 h已明显增高,4 h仍持续增高,明显高于正常对照组(P<0.05),6 h降至正常。BNP、hsCRP水平于胸痛发作2 h显著升高,于12 h达到峰值。结论 IMA、BNP、hsCRP的表达与ACS密切相关,在ACS早期诊断中具有很高的应用价值。%Objective To investigate the application of ischemia modified albumin (IMA),B-type natriuretic peptide and hypersensitive C-reactive protein in early diagnosis of acute coronary syndromes(ACS). Methods The 96 cases of ACS from Qingdao Municipal hospital,including 42 cases of acute myocardial infarction(AMI),54 cases of unstable angina pectoris (UAP). Then their blood samples were collected at 2,4,6,12,18 and 24h after chest pain episode,50 healthy volunteers were as control;Detection of the content of IMA,BNP and hsCRP was done to give reasonable suggestions on the early diagnosis of ACS about them. Results At 2 hours after the onset of chest pain in patients with ACS,the level of IMA significantly increased;At 4 hours,it still increased and was significantly higher than that in control group until downing to normal at 6 hours. The BNP and hsCRP significantly increased at 2 hours,at 12 hours to reach peak. Conclusion The expression of IMA,BNP and hsCRP is closely related to ACS. It also has very high application value in the diagnosis of early ACS.

  20. The plastidic DEAD-box RNA helicase 22, HS3, is essential for plastid functions both in seed development and in seedling growth.

    Science.gov (United States)

    Kanai, Masatake; Hayashi, Makoto; Kondo, Maki; Nishimura, Mikio

    2013-09-01

    Plants accumulate large amounts of storage products in seeds to provide an energy reserve and to supply nutrients for germination and post-germinative growth. Arabidopsis thaliana belongs to the Brassica family, and oil is the main storage product in Arabidopsis seeds. To elucidate the regulatory mechanisms of oil biosynthesis in seeds, we screened for high density seeds (heavy seed) that have a low oil content. HS3 (heavy seed 3) encodes the DEAD-box RNA helicase 22 that is localized to plastids. The triacylglycerol (TAG) content of hs3-1 seeds was 10% lower than that of wild-type (WT) seeds, while the protein content was unchanged. The hs3-1 plants displayed a pale-green phenotype in developing seeds and seedlings, but not in adult leaves. The HS3 expression level was high in developing seeds and seedlings, but was low in stems, rosette leaves and flowers. The plastid gene expression profile of WT developing seeds and seedlings differed from that of hs3-1 developing seeds and seedlings. The expression of several genes was reduced in developing hs3-1 seeds, including accD, a gene that encodes the β subunit of carboxyltransferase, which is one component of acetyl-CoA carboxylase in plastids. In contrast, no differences were observed between the expression profiles of WT and hs3-1 rosette leaves. These results show that HS3 is essential for proper mRNA accumulation of plastid genes during seed development and seedling growth, and suggest that HS3 ensures seed oil biosynthesis by maintaining plastid mRNA levels.

  1. Application of SWAT-HS, a lumped hillslope model to simulate hydrology in the Cannonsville Reservoir watershed, New York

    Science.gov (United States)

    Hoang, Linh; Schneiderman, Elliot; Mukundan, Rajith; Moore, Karen; Owens, Emmet; Steenhuis, Tammo

    2017-04-01

    Surface runoff is the primary mechanism transporting substances such as sediments, agricultural chemicals, and pathogens to receiving waters. In order to predict runoff and pollutant fluxes, and to evaluate management practices, it is essential to accurately predict the areas generating surface runoff, which depend on the type of runoff: infiltration-excess runoff and saturation-excess runoff. The watershed of Cannonsville reservoir is part of the New York City water supply system that provides high quality drinking water to nine million people in New York City (NYC) and nearby communities. Previous research identified saturation-excess runoff as the dominant runoff mechanism in this region. The Soil and Water Assessment Tool (SWAT) is a promising tool to simulate the NYC watershed given its broad application and good performance in many watersheds with different scales worldwide, for its ability to model water quality responses, and to evaluate the effect of management practices on water quality at the watershed scale. However, SWAT predicts runoff based mainly on soil and land use characteristics, and implicitly considers only infiltration-excess runoff. Therefore, we developed a modified version of SWAT, referred to as SWAT-Hillslope (SWAT-HS), which explicitly simulates saturation-excess runoff by redefining Hydrological Response Units (HRUs) based on wetness classes with varying soil water storage capacities, and by introducing a surface aquifer with the ability to route interflow from "drier" to "wetter" wetness classes. SWAT-HS was first tested at Town Brook, a 37 km2 headwater watershed draining to the Cannonsville reservoir using a single sub-basin for the whole watershed. SWAT-HS performed well, and predicted streamflow yielded Nash-Sutcliffe Efficiencies of 0.68 and 0.87 at the daily and monthly time steps, respectively. More importantly, it predicted the spatial distribution of saturated areas accurately. Based on the good performance in the Town Brook

  2. Relationship between hs-CRP, proMMP-1, TIMP-1 and coronary plaque morphology: intravascular ultrasound study

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xing-wei; ZHOU Liang; YE Xian-hua; XU Jian; GE Jun-bo; YANG Jian-min; GE Lei; WANG Ning-fu; GAO Yan; LI Pei-zhang; PAN Hao; TONG Guo-xin

    2006-01-01

    Background Rupture of unstable plaque with subsequent thrombus formation is the common pathophysiological substrate of the acute coronary syndrome (ACS). It is of potential significance to explore the blood indexes predicting plaque characteristics. Little studies have focused on this field. Therefore we investigated the relationship between hypersensitive C-reactive protein (hs-CRP), pro-matrix metalloproteinase-1 (proMMP-1),tissue inhibitors of matrix metalloproteinase-1 (TIMP-1) and coronary plaque morphology.Methods Intravascular ultrasound (IVUS) examination was done in 152 patients with confirmed coronary heart disease before percutaneous coronary intervention from February 2003 to July 2005. Plasma samples of arterial blood were collected prior to the procedure. The level of hs-CRP, proMMP-1 and TIMP-1 were respectively measured by enzyme-linked immunosorbent assay (ELISA).Results Unstable and ruptured plaque were found more frequently in patients with acute myocardial infarction and unstable angina. External elastic membrane cross-sectional area (EEM CSA), plaque area, lipid pool area and plaque burden were significantly larger in ruptured and unstable plaque group. Positive remolding, thinner frequent in ruptured and unstable plaque group. The levels of plasma hs-CRP, proMMP-1 and TIMP-1 were higher in ruptured plaque group. hs-CRP>8.94 mg/L was used to predict ruptured plaque with a ROC curve area of 0.76 [95% confidence interval (CI), 67.0%-85.8%], sensitivity of 71.8%, specificity of 77.0% and accuracy of 69.2% (P<0.01), similarly for proMMP-1>0.12 ng/ml with a ROC curve area of 0.69 [95% CI, 58.2%-80.2%], sensitivity of 69.2%, specificity of 75.2% and accuracy of 66.2% (P<0.01), and TIMP-1>83.45 ng/ml with a ROC curve area of 0.67 [95% CI, 56.2%-78.3%], sensitivity of 66.7%, specificity of 61.9% and accuracy of 66.2% (P<0.01).Conclusion The plaque characteristics correlate with the clinical presentation. The elevation of hs-CRP,proMMP-1 and

  3. 血清HS-CRP LDL-C/HDL-C在冠心病中的临床意义

    Institute of Scientific and Technical Information of China (English)

    徐文星; 蒋廷波

    2013-01-01

      目的通过检测冠心病(CHD)患者体内超敏C-反应蛋白(hs-CRP)、低密度脂蛋白(LDL-C)和高密度脂蛋白(HDL-C)水平,研究HS-CRP与LDL-C/HDL-C冠心病及冠心病病变程度中的临床意义.方法根据冠状动脉(简称冠脉)造影结果的Gensini评分,将患者分为四组:(1)冠脉造影正常组(64例):Gensini评分0分.(2)冠脉轻度病变组(61例):Gensini评分30分.比较四组hS-CRP及LDL-C/HDL-C升高与冠心病的相关程度.结果冠心病组患者的hs-CRP浓度和LDL-C/HDL-C均显著高于冠脉正常组(P30),the forth group:the normal group(=0),and Measured their hs-CRP,LDL-C/HDL-C.Results The serum hs-CRP levels and LDL-C/HDL-C in patients with coronary heart disease were significantly increased than those in normal group(P<0.05).In addition,hS - CRP levels in coronary artery coronary moderate lesion group is significantly higher than mild coronary artery lesion group(P<0.05);Severe lesion group is significantly higher than coronary moderate lesion group(P<0.05).Conclusion hS-CRP and LDL-C/HDL-C and coronary artery lesions are closely related,and hS-CRP is associated with the severity of the disease(according to the Gensini score),which can be used as to coronary artery lesion degree of estimation index.Although the serum levels of LDL-C/HDL-C are closely related to coronary artery lesions, it is hard to predict the degree of pathological changes fully accordingly to the such index.

  4. Locus control region HS2 point mutations are generally not responsible for elevated fetal hemoglobin expression of sickle cell patients

    Energy Technology Data Exchange (ETDEWEB)

    Gilman, J.G. [Montefiore Medical Center/Albert Einstein College of Medicine, Bronx, NY (United States); Brinson, E.C.; Milner, P.M. [Medical College of Georgia, Augusta (United States)] [and others

    1994-09-01

    The locus control region (LCR), composed of four hypersensitive sites (HS1-4) 5{prime} of the {epsilon} globin gene, confers strong, copy-number dependent expression on globin genes in transgenic mice. Several {beta}-globin gene cluster haplotypes carry the sickle cell gene, and show variable levels of fetal hemoglobin (Hb F) expression in association with DNA sequence differences in HS2, {gamma} and {beta} globin promoters, and {gamma}IVSII: The Senegal (SEN or No. 3) haplotype generally has high (>10%) Hb F, Benin (BEN or No. 19) has intermediate Hb F (but some low and some high), and Banu (BAN or No. 20) generally has low Hb F. Huisman and colleagues have proposed that `factors produced under conditions of hematopoietic stress, together with genetic determinants on the haplotype-3 like LCR sequences, allow for high level expression of {gamma} globin genes`. We have now used slot blot to screen high Hb F (>9.5%) and low Hb F cases for two of the three HS2 point mutations described by Oener et al. Comparing eight high Hb F BEN/BEN with two low Hb F BEN/BEN, all ten had the BEN mutations considered by Oener et al. to be associated with low Hb F. Comparing three high Hb F BEN/BAN with two low Hb F BEN/BAN, all five were heterozygous at three positions; this is consistent with BEN having G and T and BAN having A at both positions. DNA sequencing of HS2 for BAN, which is generally associated with low HB F, showed that the point mutations at all three positions were those seen in SEN (generally high Hb F); only the AT repeat region showed major differences, confirming results of Huisman and colleagues. Hence, if there is any effect of HS2 of the Senegal sickle cell haplotype in causing elevated Hb F under hematopoietic stress, it must be due to specific variation in the AT repeat region, which Oener et al. have suggested may bind a silencer.

  5. Significance of IL-17 and hs-CRP detected in patients with acute aoronary syndrome%IL-17、hs-CRP联合检测在急性冠脉综合征中的意义

    Institute of Scientific and Technical Information of China (English)

    韦德宇; 丁晓梅; 苏浩; 胡昊; 陈东

    2011-01-01

    Aim To observe the changes of IL-17 and hs-CRP in patients with acute coronary syndrome (ACS) ,and whether there was correlation between the two or not,and to investigate the relationship between IL-17 and the instability of plaque. Methods 113 patients were divided into 4 groups:40 cases with acute myocardial infarction (AMI) ,31 cases with unstable angina (UA) ,18 patients with sta ble angina pectoris (SAP) ,and 24 patients with chest pain syndrome (CPS). All were taken blood from immediately after admission in, and centrifugal samples stored in -80℃ refrigerator to detect the serum levels of IL-17,hs-CRP,cTNI,CK-MB by ELISA. Results AMI and UA in peripheral blood levels of IL-17 [ (37.04 ± 9.92) , (30.11 ± 5.67) ng · L -1 ] were significantly higher than the SAP (20.95 ± 5.08) ng · L -1 and the CPS ( 15.81 ± 7.65) ng · L -1 (P < 0.01). Levels of hs-CRP in AMI and UA [ (39.21 ± 14.42), ( 11.57 ± 3.71) mg · L -1 ] ,were significantly higher than the SAP (3.53 ± 4.45) mg · L -1 and the CPS gr(2.94 ± 3.97) mg · L -1 (P < 0.05). IL-17 and hs-CRP were positively correlated(r 0. 364,P < 0. 001). Conclusion The serum levels of IL-17 were significantly increased and was positively correlated with hs-CRP. We suggested that IL-17 becorrelated with the instability of atheroscle rotic plaque,and also involved in the pathogenesis of ACS.%目的 观察IL-17、hs-CRP在急性冠脉综合征(ACS)患者血清中的水平变化,同时观察两者是否存在相关性,推测IL-17与斑块不稳定性的关系.方法 选择113例患者分为四组:急性心肌梗死(AMI)40例,不稳定型心绞痛(UA)31例,稳定型心绞痛(SAP)18例,胸痛综合征(CPS)24例.患者入院后立即留取静脉血标本离心,存于-80℃的冰箱,采用ELISA法集中检测患者血清中的IL-17、hs-CRP、cTNI、CK-MB水平.结果 AMI组和UA组外周血清IL-17水平分别为(37.04±9.92)、(30.11±5.67)ng·L-1,明显高于SAP组的(20.95±5.08)ng·L-1和CPS组(15.81±7.65)ng

  6. Periodontal Status in Patients with Coronary Heart Disease and Fg and hs-CRP Correlation.%冠心病患者的牙周状况与Fg和hs-CRP相关性的研究

    Institute of Scientific and Technical Information of China (English)

    钟慧敏; 常春荣; 张建全; 俞宁; 潘亚萍

    2011-01-01

    目的:通过牙周临床检查和血生化指标检测,观察冠心病患者的牙周状况,探讨纤维蛋白原和高敏C反应蛋白在两者间的相关关系.方法:按照冠心病及慢性牙周炎临床诊断标准随机选择200名研究对象,年龄35~80岁,分为急性冠脉综合征(ACS)组50人,慢性冠脉病(CAD)组50人,单纯慢性牙周炎(CP)组50人,健康(H)对照组50人,每组都进行牙周探诊深度(PD)、临床附着丧失度(CAL)、龈沟出血指数(SBI)和简化卫生指数(OHI-S)检查,采集清晨静脉血检测Fg和hs-CRP水平.结果:PD、CAL、OHI-S在ACS组中最高,与CP组比较有显著性差异(P<0.001);SBI在CAD组中最高,ACS组与CP组比较有显著性差异(P<0.001);Fg和hs-CRP在ACS组中测定值最高,与CP组比较有显著性差异(P<0.05);血生化指标Fg和hs-CRP的水平与牙周临床指标PD、CAL、SBI、OHI-S比较呈正相关(P<0.01).结论:冠心病患者无论是急性期还是稳定期牙周状况都比单纯慢性牙周炎患者严重;PD、CAL、OHI-S的升高可引起血清中Fg和hs-CRP水平的变化,牙周临床指标的改变可能与冠心病的急性发作和加重冠心病病程有关.%Objective: To observe the periodontal condition in patients with coronary heart disease and relationship between hs-CRP and Fg by periodontal clinical examination and serum biochemical parameters.Methods: The selected 200 subjects were aged 35-89 years old.A group of acute coronary syndrome was 50 persons.A group of chronic coronary artery disease was 50 persons.A group of simple chronic periodonititis was 50 persons.A healthy control group was 50 persons.Each group was checked probing depth (PD), clinical attachment loss degrees (CAL), gingival sulcus bleeding index (SBI) and simplified health index (OHI-S).They were also collected blood in the morning to test the levels of hs-CRP and Fg, compared the levels of periodontal clinical parameters and difference between Fg and hs-CRP.Results: PD, CAL, OHI

  7. 新核素~(263)Hs,~(260)Bh及其α衰变链上核素的半衰期%Half-lives of the newly synthesized nuclei in ~(263)Hs and ~(260)Bh α-decay chains

    Institute of Scientific and Technical Information of China (English)

    苏昕宁; 张鸿飞; 左维; 李君清

    2009-01-01

    The tunneling probability of potential barrier of the newly synthesized superheavy nuclei starting from ~(263)Hs and ~(260)Bh was calculated with the Generalized Liquid Drop Model (GLDM) connected with WKB approximation and their half-lives studied. The calculated results that are in agreement with the experimental data show that the α-decay half-lives of superheavy nuclei with the Generalized Liquid Drop Model connecting with WKB approximation can be successfully, applied in the study of superheavy nuclei and describe the α-decay of superheavy nuclei. The results also indicate that the syntheses of new nuclei ~(260)Bh and ~(263)Hs and the measurements of their α-decay half-lives are reliable.%运用推广的液滴模型(GLDM)并结合量子力学中的WKB方法计算了新核素~(263)Hs,~(260)Bh及其α衰变链上各核素的势垒贯穿概率,对该链上各原子核的α衰变半衰期进行了研究.计算结果表明:利用推广的液滴模型结合WKB方法计算出的α衰变半衰期可以很好地符合在超重核区的实验值,验证了推广的液滴模型在超重核区的适用性,能够很好地描述超重核的α衰变.同时,计算表明新核素~(260)Bh和~(263)Hs的合成及其半衰期的测量是可靠的.

  8. Hs-exosomes致敏树突状细胞介导的抗卵巢癌免疫治疗的实验研究

    Institute of Scientific and Technical Information of China (English)

    张峰琴

    2012-01-01

    目的检测卵巢癌细胞的外排小体Hs-exosomes致敏树突状细胞DC诱导T淋巴细胞CTL特异性杀伤卵巢癌细胞的能力。方法:分离卵巢癌细胞株2780释放的Hs-exosomes,将其致敏DC并与T淋巴细胞共培养,检测CTL体外细胞毒活性。结果:Hs-exosomes致敏DC激活CTL的抗肿瘤能力显著高于未致敏DC组。结论:2780细胞释放的Hs-exosomes致敏DC激活T淋巴细胞对肿瘤具有很强的杀伤作用。

  9. Transitioning high sensitivity cardiac troponin I (hs-cTnI) into routine diagnostic use: More than just a sensitivity issue

    LENUS (Irish Health Repository)

    Lee, Graham R

    2016-04-01

    High sensitivity cardiac troponin T and I (hs-cTnT and hs-cTnI) assays show analytical, diagnostic and prognostic improvement over contemporary sensitive cTn assays. However, given the importance of troponin in the diagnosis of myocardial infarction, implementing this test requires rigorous analytical and clinical verification across the total testing pathway. This was the aim of this study.

  10. A comparison of osteoprotegerin with adiponectin and high-sensitivity C-reactive protein (hsCRP) as a marker for insulin resistance.

    LENUS (Irish Health Repository)

    O'Sullivan, Eoin P

    2013-01-01

    Insulin resistance (IR) is associated with low adiponectin and elevated high sensitivity C-reactive protein (hsCRP). Osteoprotegerin (OPG) has been shown to be elevated in type 2 diabetes, but whether it reflects underlying IR is unclear. We aimed to compare the ability of serum OPG with adiponectin and hsCRP to act as a marker for IR in individuals with normal and abnormal glucose tolerance.

  11. Kinetics and time dependence of the differential agglutination of acetone [AC]- and formalin [HS]-fixed Toxoplasma tachyzoites by serum of mice with experimental toxoplasmosis.

    Science.gov (United States)

    Ali, Nehad Mahmoud; Habib, Khaled Sayed Mohamed

    2012-04-01

    Researches to specify a serologic diagnostic test capable of determining the stage of toxoplasmosis, whether recent or latent, have been hampered by lack of knowing the real time of infection. Studying the precise kinetics of the differential agglutination of acetone [AC]-fixed versus that of formalin [HS]-fixed tachyzoites (differential agglutination test or AC/HS test) by sera of mice during the course of toxoplasmosis and assessment of its value in the differentiation between recent and latent infections in mice were the aims of the present work. Experimental toxoplasmosis was induced in mice, sera were collected sequentially and AC/HS test, FAST-ELISA to determine levels of IgM and IgG and microscopic examination of brain for Toxoplasma cysts were done. Both AC and HS specific patterns in the AC/HS test were noted to be dependent on the time from the onset of infection. Acute patterns of the AC/HS test were observed early in infection till before the appearance of brain cysts. Non-acute patterns were obtained late on 28th day post infection coinciding with the disappearance of IgM, persistence of IgG and presence of cysts in brains. The AC antibody was high in the recent phase of infection, and then it declined to be replaced by high sustained level of HS antibody late in infection. In conclusion, in the presence of both IgM and IgG, the appearance of either equivocal pattern or the non-acute pattern in the AC/HS test is significant in ruling out acute infection in mice.

  12. Interference of Co-Amplified Nuclear Mitochondrial DNA Sequences on the Determination of Human mtDNA Heteroplasmy by Using the SURVEYOR Nuclease and the WAVE HS System

    OpenAIRE

    Hsiu-Chuan Yen; Shiue-Li Li; Wei-Chien Hsu; Petrus Tang

    2014-01-01

    High-sensitivity and high-throughput mutation detection techniques are useful for screening the homoplasmy or heteroplasmy status of mitochondrial DNA (mtDNA), but might be susceptible to interference from nuclear mitochondrial DNA sequences (NUMTs) co-amplified during polymerase chain reaction (PCR). In this study, we first evaluated the platform of SURVEYOR Nuclease digestion of heteroduplexed DNA followed by the detection of cleaved DNA by using the WAVE HS System (SN/WAVE-HS) for detectin...

  13. Mitochondrial HMG to CoA synthase (mHS): cDNA cloning in human, mouse and C. elegans, mapping to human chromosome 1p12-13 and partial human genomic cloning

    Energy Technology Data Exchange (ETDEWEB)

    Boukaftane, Y.; Robert, M.F.; Mitchell, G.A. [Hopital Sainte-Justine, Montreal, Quebec (Canada)]|[Kingston General Hospital, Ontario (Canada)] [and others

    1994-09-01

    mHS catalyzes the rate-limiting first step of ketogenesis in the liver. A cytoplasmic HS isozyme, encoded by another gene, catalyzes an early step in cholesterol synthesis. Starting from a rat mHS cDNA obtained by RT-PCR from the published rat cDNA sequence, we obtained and sequenced human and mouse cDNAs spanning the entire coding sequence of natural human and mouse mHS, as well as sequencing C. elegans HS-like cDNA. Consensus sequences for 3 mitochondrial and 4 cytoplasmic HSs were created and compared to invertebrate HS sequences. We found high conversation in the active site and at other regions presumably important for HS function. We mapped the mHS locus, HMGCS2 by in situ hybridization to chromosome 1P12-13, in contrast to the human cHS locus (HMGCS1) known to be on chromosome 5p13. Comparative mapping results suggest that these two chromosomal regions may be contiguous in other species, constant with a recent gene duplication event. Furthermore, we have characterized a human genomic mHS subclone containing 4 mHS exons, and found the position of all splice junctions to be identical to that of the hamster cHS gene except for one site in the 3{prime} nontranslated region. We calculate that the mHS and cHS genes were derived from a common ancestor 400-700 Myrs ago, implying that ketogenesis from fat may have become possible around the time of emergence of vertebrates ({approximately}500 Myr ago). Ketogenesis has evolved into an important pathway of energy metabolism, and we predict the mHS deficiency may prove to be responsible for some as yet explained cases of Reye-like syndromes in humans. This hypothesis can now be tested at the molecular level without the necessity of obtaining hepatic tissue.

  14. Galectin 1 Proangiogenic and Promigratory Effects in the Hs683 Oligodendroglioma Model Are Partly Mediated through the Control of BEX2 Expression

    Directory of Open Access Journals (Sweden)

    Marie Le Mercier

    2009-05-01

    Full Text Available We have previously reported that galectin 1 (Gal-1 plays important biological roles in astroglial as well as in oligodendroglial cancer cells. As an oligodendroglioma model, we make use of the Hs683 cell line that has been previously extensively characterized at cell biology, molecular biology, and genetic levels. Galectin 1 has been shown to be involved in Hs683 oligodendroglioma chemoresistance, neoangiogenesis, and migration. Down-regulating Gal-1 expression in Hs683 cells through targeted small interfering RNA provokes a marked decrease in the expression of the brain-expressed X-linked gene: BEX2. Accordingly, the potential role of BEX2 in Hs683 oligodendroglioma cell biology has been investigated. The data presented here reveal that decreasing BEX2 expression in Hs683 cells increases the survival of Hs683 orthotopic xenograft-bearing mice. Furthermore, this decrease in BEX2 expression impairs vasculogenic mimicry channel formation in vitro and angiogenesis in vivo, and modulates glioma cell adhesion and invasive features through the modification of several genes previously reported to play a role in cancer cell migration, including MAP2, plexin C1, SWAP70, and integrin β6. We thus conclude that BEX2 is implicated in oligodendroglioma biology.

  15. Galectin 1 Proangiogenic and Promigratory Effects in the Hs683 Oligodendroglioma Model Are Partly Mediated through the Control of BEX2 Expression1

    Science.gov (United States)

    Mercier, Marie Le; Fortin, Shannon; Mathieu, Véronique; Roland, Isabelle; Spiegl-Kreinecker, Sabine; Haibe-Kains, Benjamin; Bontempi, Gianluca; Decaestecker, Christine; Berger, Walter; Lefranc, Florence; Kiss, Robert

    2009-01-01

    We have previously reported that galectin 1 (Gal-1) plays important biological roles in astroglial as well as in oligodendroglial cancer cells. As an oligodendroglioma model, we make use of the Hs683 cell line that has been previously extensively characterized at cell biology, molecular biology, and genetic levels. Galectin 1 has been shown to be involved in Hs683 oligodendroglioma chemoresistance, neoangiogenesis, and migration. Down-regulating Gal-1 expression in Hs683 cells through targeted small interfering RNA provokes a marked decrease in the expression of the brain-expressed X-linked gene: BEX2. Accordingly, the potential role of BEX2 in Hs683 oligodendroglioma cell biology has been investigated. The data presented here reveal that decreasing BEX2 expression in Hs683 cells increases the survival of Hs683 orthotopic xenograft-bearing mice. Furthermore, this decrease in BEX2 expression impairs vasculogenic mimicry channel formation in vitro and angiogenesis in vivo, and modulates glioma cell adhesion and invasive features through the modification of several genes previously reported to play a role in cancer cell migration, including MAP2, plexin C1, SWAP70, and integrin β6. We thus conclude that BEX2 is implicated in oligodendroglioma biology. PMID:19412433

  16. Galectin 1 proangiogenic and promigratory effects in the Hs683 oligodendroglioma model are partly mediated through the control of BEX2 expression.

    Science.gov (United States)

    Le Mercier, Marie; Fortin, Shannon; Mathieu, Véronique; Roland, Isabelle; Spiegl-Kreinecker, Sabine; Haibe-Kains, Benjamin; Bontempi, Gianluca; Decaestecker, Christine; Berger, Walter; Lefranc, Florence; Kiss, Robert

    2009-05-01

    We have previously reported that galectin 1 (Gal-1) plays important biological roles in astroglial as well as in oligodendroglial cancer cells. As an oligodendroglioma model, we make use of the Hs683 cell line that has been previously extensively characterized at cell biology, molecular biology, and genetic levels. Galectin 1 has been shown to be involved in Hs683 oligodendroglioma chemoresistance, neoangiogenesis, and migration. Down-regulating Gal-1 expression in Hs683 cells through targeted small interfering RNA provokes a marked decrease in the expression of the brain-expressed X-linked gene: BEX2. Accordingly, the potential role of BEX2 in Hs683 oligodendroglioma cell biology has been investigated. The data presented here reveal that decreasing BEX2 expression in Hs683 cells increases the survival of Hs683 orthotopic xenograft-bearing mice. Furthermore, this decrease in BEX2 expression impairs vasculogenic mimicry channel formation in vitro and angiogenesis in vivo, and modulates glioma cell adhesion and invasive features through the modification of several genes previously reported to play a role in cancer cell migration, including MAP2, plexin C1, SWAP70, and integrin beta(6). We thus conclude that BEX2 is implicated in oligodendroglioma biology.

  17. A potent and Kv1.3-selective analogue of the scorpion toxin HsTX1 as a potential therapeutic for autoimmune diseases

    Science.gov (United States)

    Rashid, M. Harunur; Huq, Redwan; Tanner, Mark R.; Chhabra, Sandeep; Khoo, Keith K.; Estrada, Rosendo; Dhawan, Vikas; Chauhan, Satendra; Pennington, Michael W.; Beeton, Christine; Kuyucak, Serdar; Norton, Raymond S.

    2014-03-01

    HsTX1 toxin, from the scorpion Heterometrus spinnifer, is a 34-residue, C-terminally amidated peptide cross-linked by four disulfide bridges. Here we describe new HsTX1 analogues with an Ala, Phe, Val or Abu substitution at position 14. Complexes of HsTX1 with the voltage-gated potassium channels Kv1.3 and Kv1.1 were created using docking and molecular dynamics simulations, then umbrella sampling simulations were performed to construct the potential of mean force (PMF) of the ligand and calculate the corresponding binding free energy for the most stable configuration. The PMF method predicted that the R14A mutation in HsTX1 would yield a > 2 kcal/mol gain for the Kv1.3/Kv1.1 selectivity free energy relative to the wild-type peptide. Functional assays confirmed the predicted selectivity gain for HsTX1[R14A] and HsTX1[R14Abu], with an affinity for Kv1.3 in the low picomolar range and a selectivity of more than 2,000-fold for Kv1.3 over Kv1.1. This remarkable potency and selectivity for Kv1.3, which is significantly up-regulated in activated effector memory cells in humans, suggest that these analogues represent valuable leads in the development of therapeutics for autoimmune diseases.

  18. Apoferritin-templated biosynthesis of manganese nanoparticles and investigation of direct electron transfer of MnNPs-HsAFr at modified glassy carbon electrode.

    Science.gov (United States)

    Rafipour, Ronak; Kashanian, Soheila; Hashemi, Sadegh; Omidfar, Kobra; Ezzati Nazhad Dolatabadi, Jafar

    2017-01-01

    Manganese nanoparticles (MnNPs) were created within horse spleen apoferritin (HsAFr) cavity nanotemplates. Transmission electron microscopy revealed the particle size to be 6 nm. Intrinsic fluorescence data showed that the mineralization acted as a quencher of the HsAFr fluorescence, and extrinsic fluorescence data revealed that the hydrophobic binding site at the surface of HsAFr was not changed. Finally, the MnNP-HsAFr was immobilized onto multiwalled carbon nanotubes entrapped into chitosan (CS) matrices by through sequential 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide and glutaraldehyde coupling. The MnNPs-HsAFr immobilized on CNT-CS/GC electrode was characterized by cyclic voltammetry. This charge transfer coefficient (α) and the exchange current (i0 ) of MnNPs-HsAFr immobilized on modified electrode in 0.1 M phosphate solution (pH 7.5) were found to be 0.57 and 0.48 μA, respectively. © 2015 International Union of Biochemistry and Molecular Biology, Inc.

  19. hs-CRP检测在儿童感染性疾病诊断中的临床应用价值

    Institute of Scientific and Technical Information of China (English)

    邹尚敏

    2014-01-01

    目的:探讨超敏C反应蛋白(HS-CRP)对儿童感染性疾病的诊断意义.方法:选择215例感染性疾病患儿,其中细菌感染组112例,病毒感染组103例,检测两组患儿血清中hs-CRP浓度.结果:病毒感染组hs-CRP值平均为(3.47±2.15)mg/L,细菌感染组hs-CRP平均值为(44.74±12.83)mg/L,两组患儿hs-CRP浓度比较有统计学差异(P<0.01).结论:hs-CRP检测可迅速对细菌感染和病毒感染做出判断,可作为诊断和鉴别诊断儿科感染性疾病的有效指标.

  20. Accurate double many-body expansion potential energy surface of HS2A2A‧) by scaling the external correlation

    Science.gov (United States)

    Lu-Lu, Zhang; Yu-Zhi, Song; Shou-Bao, Gao; Yuan, Zhang; Qing-Tian, Meng

    2016-05-01

    A globally accurate single-sheeted double many-body expansion potential energy surface is reported for the first excited state of HS2 by fitting the accurate ab initio energies, which are calculated at the multireference configuration interaction level with the aug-cc-pVQZ basis set. By using the double many-body expansion-scaled external correlation method, such calculated ab initio energies are then slightly corrected by scaling their dynamical correlation. A grid of 2767 ab initio energies is used in the least-square fitting procedure with the total root-mean square deviation being 1.406 kcal·mol-1. The topographical features of the HS2(A2A‧) global potential energy surface are examined in detail. The attributes of the stationary points are presented and compared with the corresponding ab initio results as well as experimental and other theoretical data, showing good agreement. The resulting potential energy surface of HS2(A2A‧) can be used as a building block for constructing the global potential energy surfaces of larger S/H molecular systems and recommended for dynamic studies on the title molecular system. Project supported by the National Natural Science Foundation of China (Grant No. 11304185), the Taishan Scholar Project of Shandong Province, China, the Shandong Provincial Natural Science Foundation, China (Grant No. ZR2014AM022), the Shandong Province Higher Educational Science and Technology Program, China (Grant No. J15LJ03), the China Postdoctoral Science Foundation (Grant No. 2014M561957), and the Post-doctoral Innovation Project of Shandong Province, China (Grant No. 201402013).

  1. HS-SPME-GC×GC-qMS volatile metabolite profiling of Chrysolina herbacea frass and Mentha spp. leaves.

    Science.gov (United States)

    Cordero, Chiara; Zebelo, Simon Atsbaha; Gnavi, Giorgio; Griglione, Alessandra; Bicchi, Carlo; Maffei, Massimo E; Rubiolo, Patrizia

    2012-02-01

    Headspace solid-phase microextraction (HS-SPME) comprehensive two-dimensional (2D) gas chromatography combined with quadrupole-mass spectrometry (GC×GC-qMS) with dedicated comparative data elaboration was applied to separate chemical patterns arising from the interaction between some Mentha species and the herbivore Chrysolina herbacea, also known as the mint bug. Upon feeding on different Mentha species (Mentha spicata L., Mentha × piperita L. and Mentha longifolia L.), C. herbacea produced frass (faeces) which were characterized by a typical volatile fraction. HS-SPME GC×GC-qMS analysis of the complex volatile fraction of both mint leaf and C. herbacea frass was submitted to advanced fingerprinting analysis of 2D chromatographic data. 1,8-Cineole, found in the leaves of all the Mentha species examined, was oxidized, and C. herbacea frass yielded high rates of several hydroxy-1,8-cineoles, including 2α-hydroxy-, 3α-hydroxy-, 3β-hydroxy- and 9-hydroxy-1,8-cineole. Upon insect feeding, several unknown oxidized monoterpenes, a p-menthane diol and three unknown phenylpropanoids were also detected in the frass volatiles. In M. longifolia, the occurrence of the monoterpene piperitenone oxide was found to be toxic and associated with insect death. The results of this work show that high throughput techniques such as HS-SPME and GC×GC-qMS fingerprint analysis are ideal tools to analyze complex volatile matrices, and provide a sensitive method for the direct comparison and chemical visualization of plant and insect emitted volatile components.

  2. Spatial dose distributions in solid tumors from {sup 186}Re transported by liposomes using HS radiochromic media

    Energy Technology Data Exchange (ETDEWEB)

    Medina, Luis A.; Rodriguez-Villafuerte, Mercedes; Martinez-Davalos, Arnulfo; Galvan, Olga O.; Brandan, Maria-Ester [Instituto de Fisica, UNAM, A.P. 20-364, Mexico (Mexico); Goins, Beth; Awasthi, Vibhudutta; Santoyo, Cristina; Phillips, William T. [University of Texas Health Science Center at San Antonio, Department of Radiology, San Antonio, TX (United States); Bao, Ande [University of Texas Health Science Center at San Antonio, Department of Radiology, San Antonio, TX (United States); University of Texas Health Science Center at San Antonio, Department of Otolaryngology-Head and Neck Surgery, San Antonio, TX (United States)

    2007-07-15

    A procedure for the measurement of spatial dose rate distribution of beta particles emitted by {sup 186}Re-liposomes in tumoral tissue, using HS GafChromic films, is presented. HNSCC xenografts were intratumorally injected with 3.7 or 11.1 MBq of {sup 186}Re-liposomes, and planar gamma camera images were acquired to determine the liposome retention in the tumor. After imaging, rats were sacrificed and tumors were excised and processed in slices; HS film sections were placed between slices and the tumor lobe was reassembled. Tumors and films were kept in the dark at 4 C for 18 h. After irradiation, films were removed and response was read using a transmission scanner. Films were analyzed to determine two-dimensional spatial dose rate distributions and cumulative dose volume histograms. Dose rate distributions were quantified using a {sup 60}Co calibration curve, the {sup 186}Re physical half-life, and a perturbation factor that takes into account the effect of the film protective layer. Dose rate distributions are highly heterogeneous with maximal dose rates about 0.4 Gy h{sup -1} in tumors injected with 3.7 MBq and 1.3 Gy h{sup -1} in tumors injected with 11.1 MBq. Dose volume histograms showed dose distributed in more than 95% and 80% of the tumor when injected with the lower and the higher activity, respectively. The described procedures and techniques have shown the potential and utility of HS GafChromic film for determination of dose rate distributions in solid tumors injected intratumorally with {sup 186}Re-liposomes. The film's structure and the liposomes' biodistribution must be taken into account to obtain quantitative dose measurements. (orig.)

  3. Evaluating the successful implementation of evidence into practice using the PARiHS framework: theoretical and practical challenges

    Directory of Open Access Journals (Sweden)

    McCormack Brendan

    2008-01-01

    Full Text Available Abstract Background The PARiHS framework (Promoting Action on Research Implementation in Health Services has proved to be a useful practical and conceptual heuristic for many researchers and practitioners in framing their research or knowledge translation endeavours. However, as a conceptual framework it still remains untested and therefore its contribution to the overall development and testing of theory in the field of implementation science is largely unquantified. Discussion This being the case, the paper provides an integrated summary of our conceptual and theoretical thinking so far and introduces a typology (derived from social policy analysis used to distinguish between the terms conceptual framework, theory and model – important definitional and conceptual issues in trying to refine theoretical and methodological approaches to knowledge translation. Secondly, the paper describes the next phase of our work, in particular concentrating on the conceptual thinking and mapping that has led to the generation of the hypothesis that the PARiHS framework is best utilised as a two-stage process: as a preliminary (diagnostic and evaluative measure of the elements and sub-elements of evidence (E and context (C, and then using the aggregated data from these measures to determine the most appropriate facilitation method. The exact nature of the intervention is thus determined by the specific actors in the specific context at a specific time and place. In the process of refining this next phase of our work, we have had to consider the wider issues around the use of theories to inform and shape our research activity; the ongoing challenges of developing robust and sensitive measures; facilitation as an intervention for getting research into practice; and finally to note how the current debates around evidence into practice are adopting wider notions that fit innovations more generally. Summary The paper concludes by suggesting that the future

  4. Ecodesign - How to Unfold the Potential Synergy between the EuP, WEEE and RoHS Directives

    DEFF Research Database (Denmark)

    Andersen, Rikke Dorothea; Remmen, Arne

    Product Policy (IPP). The approach aims at promoting measures to reduce the environmental impacts of products. Since the IPP approach was introduced around 2000, several instruments have been implemented; the RoHS, WEEE and the EuP Directive as well as the European ecolabel and the Energy labelling...... Directive. The focus of this paper is the potential synergy between the three normative, so-called eco-design directives, and to what extent the EU has accomplished to integrate eco-design in the different directives and voluntary instruments....

  5. A novel erythromycin resistance plasmid from Bacillus sp. strain HS24, isolated from the marine sponge Haliclona simulans.

    Directory of Open Access Journals (Sweden)

    Teresa M Barbosa

    Full Text Available A better understanding of the origin and natural reservoirs of resistance determinants is fundamental to efficiently tackle antibiotic resistance. This paper reports the identification of a novel 5.8 kb erythromycin resistance plasmid, from Bacillus sp. HS24 isolated from the marine sponge Haliclona simulans. pBHS24B has a mosaic structure and carries the erythromycin resistance gene erm(T. This is the first report of an erythromycin resistance plasmid from a sponge associated bacteria and of the Erm(T determinant in the genus Bacillus.

  6. Heat perception and aversive learning in honey bees: putative involvement of the thermal/chemical sensor AmHsTRPA

    Directory of Open Access Journals (Sweden)

    Pierre eJunca

    2015-11-01

    Full Text Available The recent development of the olfactory conditioning of the sting extension response (SER has provided new insights into the mechanisms of aversive learning in honeybees. However, until now, very little information has been obtained concerning US detection and perception in this aversive conditioning. In the initial version of SER conditioning, bees learned to associate an odor CS with an electric shock US. Recently, we proposed a modified version of SER conditioning, in which thermal stimulation with a heated probe is used as US. This procedure has the advantage of allowing topical US applications virtually everywhere on the honeybee body. In this study, we made use of this possibility and mapped thermal responsiveness on the honeybee body, by measuring workers' SER after applying heat on 41 different structures. We then show that bees can learn the CS-US association even when the heat US is applied on body structures that are not prominent sensory organs, here the vertex (back of the head and the ventral abdomen. Next, we used a neuropharmalogical approach to evaluate the potential role of a recently described Transient Receptor Potential (TRP channel, HsTRPA, on peripheral heat detection by bees. First, we applied HsTRPA activators

  7. Quality changes during storage of cooked and sliced meat products measured with PTR-MS and HS-GC-MS.

    Science.gov (United States)

    Holm, E S; Adamsen, A P S; Feilberg, A; Schäfer, A; Løkke, M M; Petersen, M A

    2013-10-01

    The changes in the VOC composition of industrially produced saveloy were measured with Proton-Transfer-Reaction Mass-Spectrometry (PTR-MS) and HeadSpace Gas chromatography-mass spectrometry (HS-GC-MS) during a six weeks storage period. A decrease in the volatile organic compounds contributing to the fresh aroma of saveloy was the main change observed with both PTR-MS and HS-GC-MS. Samples of four other types of cooked and sliced meat product were measured with PTR-MS in the middle and at the end of the four week shelf-life period. These measurements showed an increase in m/z 69, 71, 87 and 89 for the pork loin and in m/z 61 for the herbal saveloy samples. These ions were assigned to the microbial spoilage markers: acetic acid, 2- and 3-methylbutanol, 2- and 3-methylbutanal, diacetyl and acetoin. Overall, this study shows that PTR-MS has potential for quality control of cooked and sliced meat products. Copyright © 2013 Elsevier Ltd. All rights reserved.

  8. Volatile profile analysis and quality prediction of Longjing tea (Camellia sinensis) by HS-SPME/GC-MS

    Institute of Scientific and Technical Information of China (English)

    Jie LIN; Yi DAI; Ya-nan GUO; Hai-rong XU; Xiao-chang WANG

    2012-01-01

    This study aimed to analyze the volatile chemical profile of Longjing tea,and further develop a prediction model for aroma quality of Longjing tea based on potent odorants.A total of 21 Longjing samples were analyzed by headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS).Pearson's linear correlation analysis and partial least square (PLS) regression were applied to investigate the relationship between sensory aroma scores and the volatile compounds.Results showed that 60 volatile compounds could be commonly detected in this famous green tea.Terpenes and esters were two major groups characterized,representing 33.89% and 15.53% of the total peak area respectively.Ten compounds were determined to contribute significantly to the perceived aroma quality of Longjing tea,especially linalool (0.701),nonanal (0.738),(Z)-3-hexenyl hexanoate (-0.785),and β-ionone (-0.763).On the basis of these 10 compounds,a model (correlation coefficient of 89.4% and cross-validated correlation coefficient of 80.4%) was constructed to predict the aroma quality of Longjing tea.Summarily,this study has provided a novel option for quality prediction of green tea based on HS-SPME/GC-MS technique.

  9. Degradation of phenanthrene by Novosphingobium sp. HS2a improved plant growth in PAHs-contaminated environments.

    Science.gov (United States)

    Rodriguez-Conde, Sara; Molina, Lázaro; González, Paola; García-Puente, Alicia; Segura, Ana

    2016-12-01

    At the same time that the European Union (EU) policy recommend to direct efforts towards reductions of heavy metals, polycyclic aromatic hydrocarbons (PAHs) and mining residues, there is the need to increase the cultivable areas within Europe to cope with the increasing demands for food and energy crops. Bioremediation is a good technique for the restoration of contaminated soils; however, it has not been used extensively because of the variability of the outcome. This variability is frequently due to a bad establishment of foreign degrading populations in soil. We have demonstrated that Novosphingobium sp. HS2aR (i) is able to compete with other root colonizers and with indigenous bacteria, (ii) is able to establish in high numbers in the contaminated environments and (iii) is able to remove more than 90 % of the extractable phenanthrene in artificially contaminated soils. Furthermore, we have demonstrated that the capacity to remove phenanthrene is linked to the ability to promote plant growth in contaminated environments. The fact that the presence of Novosphingobium sp. HS2aR improves the growth of plants in contaminated soil suggests that it may be a useful strain for utilization in amelioration of soil quality while improving the growth of economically important energy crops, thus adding value to the bioremediation strategy.

  10. Effect of hs-CRP level and nutritional status on pulmonary function in patients with maintenance hemodialysis

    Institute of Scientific and Technical Information of China (English)

    Ru Lei

    2016-01-01

    Objective:To explore the effect of hs-CRP level and nutritional status on the pulmonary function in patients with maintenance hemodialysis (MHD). Methods: A total of 30 patients with chronic renal failure (CRF) who were admitted in our hospital from August, 2014 to August, 2015 for hemodialysis were included in the study. A volume of 4mL morning fasting elbow venous blood before and after hemodialysis was extracted, and was then centrifuged for serum. The levels of Hb, Alb, and PA were detected. VC, FVC, FEV1, FEV1/FVC, PEF, and MMEF were determined. Results: The levels of Hb, Alb, and PA after hemodialysis were significantly higher than those before hemodialysis (P<0.05). VC, FVC, FEV1, FEV1/FVC, PEF, and MMEF after hemodialysis were significantly improved when compared with before treatment (P<0.05). The comparison of VC, FVC, FEV1, FEV1/FVC, PEF, and MMEF among patients with different levels of hs-CRP was statistically significant (P<0.05). Conclusions:The reduction of pulmonary function in different degrees exists in MHD patients. MHD is kind of effective method to improve the pulmonary function in patients with CRF. The effective improvement of nutritional status and the reduction of in vivo inflammatory reaction in patients with CRF in the clinic can relieve the deterioration of pulmonary function to a certain degree so that to improve the ventilation function and enhance the living quality.

  11. HS-SPME-GC-MS ANALYSIS OF VOLATILE AND SEMI-VOLATILE COMPOUNDS FROM DRIED LEAVES OF Mikania glomerata Sprengel

    Directory of Open Access Journals (Sweden)

    Esmeraldo A. Cappelaro

    2015-03-01

    Full Text Available This paper reports on the identification of volatile and semi-volatile compounds and a comparison of the chromatographic profiles obtained by Headspace Solid-Phase Microextraction/Gas Chromatography with Mass Spectrometry detection (HS-SPME-GC-MS of dried leaves of Mikania glomerata Sprengel (Asteraceae, also known as 'guaco.' Three different types of commercial SPME fibers were tested: polydimethylsiloxane (PDMS, polydimethylsiloxane/divinylbenzene (PDMS/DVB and polyacrylate (PA. Fifty-nine compounds were fully identified by HS-SPME-HRGC-MS, including coumarin, a marker for the quality control of guaco-based phytomedicines; most of the other identified compounds were mono- and sesquiterpenes. PA fibers performed better in the analysis of coumarin, while PDMS-DVB proved to be the best choice for a general and non-selective analysis of volatile and semi-volatile guaco-based compounds. The SPME method is faster and requires a smaller sample than conventional hydrodistillation of essential oils, providing a general overview of the volatile and semi-volatile compounds of M. glomerata.

  12. Metafore spaziali della conoscenza di sé nei dialoghi di H.S. Skovoroda. Fonti patristiche

    Directory of Open Access Journals (Sweden)

    Maria Grazia Bartolini

    2009-01-01

    Full Text Available Spatial Metaphors for Self-knowledge in the dialogues of H.S Skovoroda. Patristic Sources In this paper the Author analyzes some spatial metaphors informing H.S. Skovoroda’s representation of self-knowledge as a dynamic process. Knowledge of the true self – which is one of the recurring themes in the Ukrainian philosopher’s oeuvre – is often portrayed as tension between open (e.g. streets, markets, the sky, the planets… and closed spaces (e.g. the house, the room, with the ‘home’ being a symbol for the soul. As man seeking communion with God has to abandon what lies outside his inner self, the authentic gnoseological process is represented as an introverted movement through symbolic space. Broadly speaking, this very opposition harks back to Philo of Alexandria and Patristic thought, whose anti-physicist stance shaped their rejection of the phenomenological world as an ‘interior vs. exterior’ contrast. Furthermore, the broader biblical context in which those spatial terms appear is also taken into account, while demonstrating Skovoroda’s partial dependence on Patristic models. The fi nal part of the paper argues that the scheme underlying Neo-platonic emanation – which is itself to be considered as an oscillation between unity and dispersion – appears to be a symbolic model for Skovoroda’s spatial representation of self-knowledge.

  13. hs-CRP is strongly associated with coronary heart disease (CHD): A data mining approach using decision tree algorithm.

    Science.gov (United States)

    Tayefi, Maryam; Tajfard, Mohammad; Saffar, Sara; Hanachi, Parichehr; Amirabadizadeh, Ali Reza; Esmaeily, Habibollah; Taghipour, Ali; Ferns, Gordon A; Moohebati, Mohsen; Ghayour-Mobarhan, Majid

    2017-04-01

    Coronary heart disease (CHD) is an important public health problem globally. Algorithms incorporating the assessment of clinical biomarkers together with several established traditional risk factors can help clinicians to predict CHD and support clinical decision making with respect to interventions. Decision tree (DT) is a data mining model for extracting hidden knowledge from large databases. We aimed to establish a predictive model for coronary heart disease using a decision tree algorithm. Here we used a dataset of 2346 individuals including 1159 healthy participants and 1187 participant who had undergone coronary angiography (405 participants with negative angiography and 782 participants with positive angiography). We entered 10 variables of a total 12 variables into the DT algorithm (including age, sex, FBG, TG, hs-CRP, TC, HDL, LDL, SBP and DBP). Our model could identify the associated risk factors of CHD with sensitivity, specificity, accuracy of 96%, 87%, 94% and respectively. Serum hs-CRP levels was at top of the tree in our model, following by FBG, gender and age. Our model appears to be an accurate, specific and sensitive model for identifying the presence of CHD, but will require validation in prospective studies. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Determination of 2-Methylisoborneol and Geosmin in Water by HS-SPME-GC-MS%HS-SPME-GC-MS法测定水中2-甲基异冰片及土臭素

    Institute of Scientific and Technical Information of China (English)

    黎莉; 曹芹; 曹旭静; 余俭

    2014-01-01

    Headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to detect the trace 2-MIB, GSM in surface water. The optimal operation conditions of SPME were obtained as follows:extraction time 30 min, extraction temperature 70℃, NaCl 30%(mass fraction),desorption temperature 250℃in GC. Internal standard method was used to quantify, concentration of 2-MIB, GSM was linear with peak area in the range of 5-100 ng/L with the correlation coefficient (r2) of 0.999 5, 0.999 6, respectively. The detection limits of 2-MIB, GSM were 0.8, 1.7 ng/L, respectively. The method was used to detect water samples, the relative standard deviations of 2-MIB, GSM were 2.6%-6.3%(n=6), and the recoveries were 92%-112%. The method is simple and fast for the determination of trace odor compounds in water.%采用顶空固相微萃取-气质联用(HS-SPME-GC-MS)的方法对地表水中2-甲基异冰片(2-MIB)和土臭素(GSM)进行分析测定。通过试验确定了HS-SPME的最佳萃取条件:萃取头为DVB/CAR/PDMS,萃取时间为30 min,萃取温度为70℃,NaCl的加入量为30%(质量分数),萃取纤维在GC上的解吸温度为250℃。用内标法进行定量,2-MIB,GSM的质量浓度在5~100 ng/L范围内与色谱峰面积呈良好的线性关系,线性相关系数(r2)分别为0.9997,0.9970,检出限分别为0.8,1.7 ng/L。采用该法对水样进行测定,2-MIB,GSM测定结果的相对标准偏差为2.6%~6.3%(n=6),加标回收率为92%~112%。该方法能简单、快速地测定水中痕量嗅味物质。

  15. HS-streamCube: Real-time multi-dimensional analysis system on network security event stream%HS-StreamCube:网络安全事件流实时多维分析系统

    Institute of Scientific and Technical Information of China (English)

    甘亮; 李润恒; 贾焰; 刘健

    2013-01-01

    大规模网络安全监控应用中,决策者应用数据流联机在线分析(Stream OLAP)技术对网络安全事件流建立流数据方(Stream Cube)进行实时分析,以了解当前网络安全状况并动态评估当前网络安全态势.由于内存容量有限,Stream Cube只关注当前时间窗口内的数据,而对于时间窗口外的过期数据则采用近似存储或简单地丢弃,所以不支持超出时间窗口范围的大时间窗口查询.针对以上缺陷,提出一种多维多层安全事件流实时分析框架HS-StreamCube,采用内存和外存两层混合存储模式实现任意时间窗口的精确查询;然后根据数据流特点重点研究两层混合存储模式下HS-StreamCube的模型、构建、存储管理和查询等;最后通过实验验证该系统的可用性和高效性.%In the applications of large-scale network security monitoring, data stream of security e-vents is analysised real-timely to acquire the characteristic of current security in the network and to assess dynamically the current security situation with Stream OLAP by building Stream Cube. Because of the limited memory capacity, Stream Cube only concerned about the current data within the time window, but expired data is stored approximately or simply discarded, so it do not support the query with time beyond the scope of current time window. We propose a real-time StreamCube-based multi-dimensional and multi-level analysis framework on security event stream, Hybrid StorageStreamCube, which is implemented by a two-tier (memory and disk) storage model. On the basis of characteristics of data stream,we focus on the modeling,building,storing and querying of HS-StreamCube within the two-tier storage model. Efficient experiments verify the availability and efficiency of the system.

  16. Effect of ultrasound combined with determination of CA19-9 and hs-CRP on the accuracy of early diagnosis of gallbladder cancer

    Directory of Open Access Journals (Sweden)

    Hang-yu ZHANG

    2014-03-01

    Full Text Available Objective  To investigate the value of ultrasound combined with tumor marker CA19-9 and high sensitivity C reactive protein (hs-CRP on the accuracy of early diagnosis of gallbladder cancer. Methods  Ninety-five patients with pathologically confirmed primary gallbladder cancer who received cholecystectomy from Jan. 2008 to Jan. 2013 in our hospital were included in the study. The study also included 51 patients with benign cystic lesions and 51 healthy adults. In all patients, results of pathological examination, B-ultrasound, serum CA19-9 and hs-CRP detection were obtained. In all the healthy adults gallbladder diseases were ruled out, but all of them received serum CA19-9 and hs-CRP determination. Results  Of 95 patients with primary gallbladder cancer, B-ultrasound showed nodules in the gallbladder lumen in 31, tumor mass in 40, and thickened wall in 24. Histological examination showed adenocarcinoma in 87 cases, adenosquamous carcinoma in 6, and squamous carcinoma in 2. There was a statistical difference in hs-CRP and CA19-9 levels between the groups (P<0.05, and the highest level appeared in gallbladder cancer group. There was a high sensitivity but low specificity of CA19-9 or hs-CRP in the diagnosis of primary gallbladder cancer. The sensitivity and specificity were 74.7% and 75.0% when primary gallbladder cancer was diagnosed with B-ultrasound. The positive values of CA19-9 and hs-CRP were ≥100U/ml and ≥6mg/L respectively, when they were combined with B-ultrasound as a single serology factor. When both B-ultrasound and serum levels of CA19-9 and hs-CRP were positive, the diagnostic sensitivity and specificity were 71.6% and 84.4% respectively. When B-ultrasound or serum CA19-9 and hs-CRP were positive, the diagnostic sensitivity and specificity were 98.9% and 66.7% respectively. Conclusions  Combination of B-ultrasound and determination of serum of CA19-9 and hs-CRP can improve the accuracy of the diagnosis of gallbladder

  17. Optimizaion of component HS-SPMEz extraction from Zhenjiang fragrance vinegar by response surface methodology%利用响应面法优化HS-SPME镇江香醋香气成分的条件

    Institute of Scientific and Technical Information of China (English)

    李国栋; 孙宗保

    2009-01-01

    为了优化镇江香醋香气成分的提取,在单因素实验的基础上,根据Box-Benhnken的中心组合实验设计原理,采用3因素3水平的响应面分析法,以提取物质的质(色)谱图总峰面积为响应值做响应面和等高线图.结果表明,HS-SPME萃取镇江香醋香气成分的最佳条件是氯化钠添加量2.5 g,萃取温度50.16℃,萃取时间39.76 min.

  18. Long-term Temozolomide Treatment Induces Marked Amino Metabolism Modifications and an Increase in TMZ Sensitivity in Hs683 Oligodendroglioma Cells

    Directory of Open Access Journals (Sweden)

    Delphine Lamoral-Theys

    2010-01-01

    Full Text Available Gliomas account for more than 50% of all primary brain tumors. The worst prognosis is associated with gliomas of astrocytic origin, whereas gliomas with an oligodendroglial origin offer higher sensitivity to chemotherapy, especially when oligodendroglioma cells display 1p19q deletions. Temozolomide (TMZ provides therapeutic benefits and is commonly used with radiotherapy in highly malignant astrocytic tumors, including glioblastomas. The actual benefits of TMZ during long-term treatment in oligodendroglioma patients have not yet been clearly defined. In this study, we have investigated the effects of such a long-term TMZ treatment in the unique Hs683 oligodendroglioma model. We have observed increased TMZ sensitivity of Hs683 orthotopic tumors that were previously treated in vitro with months of progressive exposure to increasing TMZ concentrations before being xenografted into the brains of immunocompromised mice. Whole-genome and proteomic analyses have revealed that this increased TMZ sensitivity of Hs683 oligodendroglioma cells previously treated for long periods with TMZ can be explained, at least partly, by a TMZ-induced p38-dependant dormancy state, which in turn resulted in changes in amino acid metabolism balance, in growth delay, and in a decrease in Hs683 oligodendroglioma cell-invasive properties. Thus, long-term TMZ treatment seems beneficial in this Hs683 oligodendroglioma model, which revealed itself unable to develop resistance against TMZ.

  19. 冠心病患者血清IL-17、IL-35及hs-CRP水平的变化及临床意义

    Institute of Scientific and Technical Information of China (English)

    李大军; 车航; 付勇

    2016-01-01

    目的:探讨IL-17、IL-35和hs-CRP在冠心病发病过程中的作用及临床意义。方法采用ELISA方法对152例冠心病患者(AMI组52例,UA组64例,SA组36例)和40例正常人(对照组)血清中IL-17、IL-35水平进行检测;免疫比浊法检测hs-CRP。结果血清中IL-17和IL-35水平呈明显负相关,IL-17与hs-CRP的水平呈正相关,IL-35与hs-CRP的水平呈负相关。结论 IL-17、IL-35和hs-CRP的水平与冠心病的发生发展密切相关,有重要临床意义。

  20. Restriction of the use of hazardous substances (RoHS in the personal computer segment: analysis of the strategic adoption by the manufacturers settled in Brazil

    Directory of Open Access Journals (Sweden)

    Ademir Brescansin

    2015-09-01

    Full Text Available The enactment of the RoHS Directive (Restriction of Hazardous Substances in 2003, limiting the use of certain hazardous substances in electronic equipment has forced companies to adjust their products to comply with this legislation. Even in the absence of similar legislation in Brazil, manufacturers of personal computers which are located in this country have been seen to adopt RoHS for products sold in the domestic market and abroad. The purpose of this study is to analyze whether these manufacturers have really adopted RoHS, focusing on their motivations, concerns, and benefits. This is an exploratory study based on literature review and interviews with HP, Dell, Sony, Lenovo, Samsung, LG, Itautec, and Positivo, using summative content analysis. The results showed that initially, global companies adopted RoHS to market products in Europe, and later expanded this practice to all products. Brazilian companies, however, adopted RoHS to participate in the government’s sustainable procurement bidding processes. It is expected that this study can assist manufacturers in developing strategies for reducing or eliminating hazardous substances in their products and processes, as well as help the government to formulate public policies on reducing risks of environmental contamination.

  1. The association of hs-CRP with fasting and postprandial plasma lipids in patients with type 2 diabetes is disrupted by dietary monounsaturated fatty acids.

    Science.gov (United States)

    Bozzetto, L; De Natale, C; Di Capua, L; Della Corte, G; Patti, L; Maione, S; Riccardi, G; Rivellese, A A; Annuzzi, G

    2013-04-01

    The aim of the study was to evaluate whether two dietary approaches recommended for diabetes mellitus and cardiovascular prevention-high-MUFA or complex carbohydrates/fiber-differently influence inflammation. A 4-week crossover study in 12 individuals with type 2 diabetes was performed. Fasting and postprandial hs-CRP plasma levels were not significantly different after a high-carbohydrate/high-fiber/low-glycemic index (CHO/fiber) and a high-MUFA diet. Compared with fasting, hs-CRP levels decreased significantly after the MUFA but not after the CHO/fiber meal. Triglyceride-rich lipoproteins were significantly lower after the CHO/fiber than the MUFA diet. At fasting and postprandially, hs-CRP correlated with triglyceride in whole plasma, chylomicrons, small and large VLDL after the CHO/fiber but not after the MUFA diet. In conclusion, a MUFA-rich diet and a carbohydrate/fiber-rich diet induced similar effects on plasma hs-CRP concentrations. However, these dietary approaches seem to influence hs-CRP levels through different mechanisms. i.e., direct acute postprandial effects by MUFA and triglyceride-rich lipoproteins mediated effects by CHO/fiber.

  2. The Level of hs-CRP in Coronary Artery Ectasia and Its Response to Statin and Angiotensin-Converting Enzyme Inhibitor Treatment

    Directory of Open Access Journals (Sweden)

    Yilmaz Ozbay

    2007-01-01

    Materials and method. We measured serum hs-CRP level in 40 CAE (26 males, mean age: 56.32±9 years and 41 O-CAD (34 males, mean age: 57.19±10 years patients referred for elective coronary angiography at baseline and after 3-month statin and ACE inhibitor treatment. Results. Plasma hs-CRP levels were significantly higher in CAE group than O-CAD group at baseline (2.68±66 mg/L versus 1,64±64, resp., P<.0001. Plasma hs-CRP levels significantly decreased from baseline 3 months later in the CE (from 2.68±0.66 mg/L to 1.2±0.53 mg/L, P<.0001 as well as in the O-CAD group (from 1.64±0.64 mg/L to 1.01±0.56 mg/L, P<.001. Conclusion. We think that hs-CRP measurement may be a good prognostic value in CAE patients as in stenotic ones. Further placebo-controlled studies are needed to evaluate the clinical significance of this decrease in hs-CRP.

  3. Impaired facial emotion recognition in patients with mesial temporal lobe epilepsy associated with hippocampal sclerosis (MTLE-HS): Side and age at onset matters.

    Science.gov (United States)

    Hlobil, Ulf; Rathore, Chaturbhuj; Alexander, Aley; Sarma, Sankara; Radhakrishnan, Kurupath

    2008-08-01

    To define the determinants of impaired facial emotion recognition (FER) in patients with mesial temporal lobe epilepsy associated with hippocampal sclerosis (MTLE-HS), we examined 76 patients with unilateral MTLE-HS, 36 prior to antero-mesial temporal lobectomy (AMTL) and 40 after AMTL, and 28 healthy control subjects with a FER test consisting of 60 items (20 each for anger, fear, and happiness). Mean percentages of the accurate responses were calculated for different subgroups: right vs. left MTLE-HS, early (age at onset Happiness recognition was significantly better in post-AMTL MTLE-HS patients compared to pre-AMTL patients while anger and fear recognition did not differ. We conclude that patients with right MTLE-HS with age at seizure onset <6 years are maximally predisposed to impaired fear recognition. In them, right AMTL does not further worsen FER abilities. Longitudinal studies comparing FER in the same patients before and after AMTL will be required to refine and confirm our cross-sectional observations.

  4. Analysis of IGF-1, Lp-PLA2, hs-CRP, Hcy levels in type 2 diabetes patients complicated with carotid atherosclerosis

    Institute of Scientific and Technical Information of China (English)

    Guan-Lun Ye; Zhuo-Zhang Luo; Wei-Ming Zheng

    2015-01-01

    Objective: To study the levels of IGF-1, Lp-PLA2, hs-CRP, Hcy in type 2 diabetes patients complicated with carotid atherosclerosis. Methods: 90 cases of type 2 diabetes patients complicated with carotid atherosclerosis in our hospital from May 2013 to August 2014 were enrolled in observation group and further divided into mild stenosis group, moderate stenosis group, severe stenosis group according to the judgment of color ultrasonography on carotid artery carotid artery stenosis, divided into mild sclerosis group, moderate sclerosis group, severe sclerosis group according to carotid artery plaque score. At the same time type 2 diabetic patients without complications in our hospital were enrolled in the control group. Then serums IGF-1, Lp-PLA2, hs-CRP, Hcy levels were assayed. Results: serum IGF-1, Lp-PLA2, hs-CRP, Hcy levels of observation group were higher than those of control group; as carotid artery stenosis and sclerosis degree got greater, serum IGF-1, Lp-PLA2, hs-CRP, Hcy levels were higher. Conclusion:serum IGF-1, Lp-PLA2, hs-CRP, Hcy levels in type 2 diabetes patients complicated with carotid atherosclerosis abnormally increased and it can reflect the degree of carotid stenosis and sclerosis.

  5. Study on the changes of blood lipids,platelet parameters and hs-CRP in different types of coronary heart disease%不同临床类型冠心病患者血脂、血小板参数及 hs-CRP 水平比较研究

    Institute of Scientific and Technical Information of China (English)

    王一平

    2016-01-01

    Objective To investigate the changes of blood lipids,platelet parameters and hs-CRP in different types of coronary heart disease (CHD).Methods A total of 271 hospitalized patients with CHD between January 2012 and Au-gust 2014 were enrolled.Patients were divided into stable angina group (SAP),unstable angina group (UAP),and a-cute myocardial infarction group (AMI).The levels of blood lipids,platelet parameters and hs-CRP in different types of CHD were examined and recorded.Results Statistically significant differences were found in TC,TG,LDL-C,hs-CRP, PLT,MPV,and PDW among three groups (P <0.05 or P <0.01).There were no differences in HDL-C and PCT a-mong them.The levels of TC,TG,LDL-C,hs-CRP,PLT,MPV,and PDW in the UAP and AMI group were significant-ly higher than that in the SAP group (P <0.05 or P <0.01).Compared with the UAP group,the levels of TC,TG, LDL-C,hs-CRP,PLT,MPV,and PDW in the AMI group were showed statistically significant differences (P <0.01). The number of cases of high hs-CRP group,intermediate hs-CRP group,and low hs-CRP group were 40.59%, 45.02%,and 14.39%,respectively.The highest group of SAP,UAP,and AMI were intermediate hs-CRP group (61.96%),high hs-CRP group (55.36%),and high hs-CRP group (88.46%),respectively.Conclusion There were different levels of blood lipids,platelet parameters and hs-CRP in different types of CHD.Testing of the blood lipids, platelet parameters and hs-CRP in patients with CHD has important value in the diagnosis and prognosis of the disease.%目的:了解不同临床类型冠心病(coronary heart disease,CHD)患者血脂、血小板参数及 hs-CRP 水平变化情况。方法选择2012年1月至2014年8月我院收治的271例,将患者分为三组:稳定型心绞痛(SAP)组163例,不稳定型心绞痛(UAP)组56例,急性心肌梗死(AMI)组52例,对不同组进行血脂、血小板参数及 hs-CRP 水平检测。结果三组患者 TC、TG、LDL-C、hs-CRP、PLT、MPV 和 PDW

  6. The Inlfuence of CE RoHS 2.0 on Medical Device and Solutions%CE RoHS 2.0对医疗器械企业的影响及企业应对措施

    Institute of Scientific and Technical Information of China (English)

    黄燕彬

    2014-01-01

    随着RoHS指令修订,RoHS 2.0被纳入为有源医疗器械产品CE认证必须符合的指令之一。2014年7月22日,M D D指令(93/42/E E C)中的有源医疗器械产品投放欧盟市场必须符合RoHS 2.0要求。2016年7月22日,IVD指令(98/79/EC)的有源医疗器械产品投放欧盟市场必须符合RoHS 2.0要求。一旦违规,企业将可能受到欧盟的处罚及造成重大损失,甚至禁止企业产品的出口,这在电子产品业界已早有先例。RoHS 2.0指令要求医疗器械企业必须建立内部过程管理控制,供应链及物料符合性评价,产品符合性验证及制作技术文档等工作,来确保医疗器械产品持续符合RoHS 的要求。SGS凭借多年专业技术及服务经验,能为医疗器械企业满足RoHS 2.0提供系统和有效措施以确保产品的合规性,帮助医疗器械企业更快进入目标市场创造更大的价值。%With the RoHS directive recast, RoHS 2.0 become one of directives that active medical device should compliance with when apply for CE certiifcation. Active medical device in the scope of Medical device directive (93/42/EEC) placing on the market must compliance with RoHS 2.0 directive requirement after July 22, 2014. And Active medical device in the scope of in vitro diagnostic medical devices (98/79/EC) placing on the market should meet the requirements of RoHS 2.0 after July 22, 2016. Manufacturer wil be disciplined by the European Union and caused signiifcant losses if not conform to RoHS, even more serious, products would prohibit of export. Event like that have been happen in electrical and electronic industry. Establish internal control process, assess compliance of materials in supplier chain, verify RoHS product conformity for RoHS and prepare technical documentation are the base requirements of RoHS. Active medical device manufacturer should meet those requirements to assure their products continue compliance with RoHS. With professional

  7. Changes in the structure of the accretion disc of HS1804+67 through the outburst cycle

    CERN Document Server

    Baptista, R; Baptista, Raymundo

    1999-01-01

    We report on the analysis of high-speed photometry of the dwarf-nova HS1804+67 through its outburst cycle with eclipse mapping techniques. Eclipse maps show evidences of the formation of a spiral structure in the disc at the early stages of the outburst and reveal how the disc expands during the rise until its fills most of the primary Roche lobe at maximum light. During the decline phase, the disc becomes progressively fainter as the cooling front moves inwards from the outer regions, until only a small bright region around the white dwarf is left at minimum light. The variable part of the uneclipsed light is possibly due to emission in a wind emanating from the inner parts of the disc. The emission from this region is sensitive to the mass accretion rate.

  8. Measurement of the isotope ratio of acetic acid in vinegar by HS-SPME-GC-TC/C-IRMS.

    Science.gov (United States)

    Hattori, Ryota; Yamada, Keita; Shibata, Hiroki; Hirano, Satoshi; Tajima, Osamu; Yoshida, Naohiro

    2010-06-23

    Acetic acid is the main ingredient of vinegar, and the worth of vinegar often depends on the fermentation of raw materials. In this study, we have developed a simple and rapid method for discriminating the fermentation of the raw materials of vinegar by measuring the hydrogen and carbon isotope ratio of acetic acid using head space solid-phase microextraction (HS-SPME) combined with gas chromatography-high temperature conversion or combustion-isotope ratio mass spectrometry (GC-TC/C-IRMS). The measurement of acetic acid in vinegar by this method was possible with repeatabilities (1sigma) of +/-5.0 per thousand for hydrogen and +/-0.4 per thousand for carbon, which are sufficient to discriminate the origin of acetic acid. The fermentation of raw materials of several vinegars was evaluated by this method.

  9. Hydrogen sulfide from a NaHS source attenuates dextran sulfate sodium (DSS)-induced inflammation via inhibiting nuclear factor-κB.

    Science.gov (United States)

    Chen, Xi; Liu, Xi-shuang

    2016-03-01

    This study investigated the alleviating effects of hydrogen sulfide (H2S), derived from sodium hydrosulfide (NaHS), on inflammation induced by dextran sulfate sodium (DSS) in both in vivo and in vitro models. We found that NaHS injection markedly decreased rectal bleeding, diarrhea, and histological injury in DSS-challenged mice. NaHS (20 μmol/L) reversed DSS-induced inhibition in cell viability in Caco-2 cells and alleviated pro-inflammation cytokine expression in vivo and in vitro, indicating an anti-inflammatory function for H2S. It was also found that H2S may regulate cytokine expression by inhibiting the nuclear factor-κB (NF-κB) signaling pathway. In conclusion, our results demonstrated that H2S alleviated DSS-induced inflammation in vivo and in vitro and that the signal mechanism might be associated with the NF-κB signaling pathway.

  10. Assessment of elder mistreatment in two American Indian samples: psychometric characteristics of the HS-EAST and the Native Elder Life-Financial Exploitation and -Neglect measures.

    Science.gov (United States)

    Jervis, Lori L; Fickenscher, Alexandra; Beals, Janette

    2014-04-01

    Although elder mistreatment among ethnic minorities is increasingly gaining attention, our empirical knowledge of this phenomenon among American Indians remains quite limited, especially with respect to measurement. The Shielding American Indian Elders (SAIE) Project used a collaborative approach to explore culturally informed measurement of elder mistreatment in two American Indian elder samples (a Northern Plains reservation and a South Central metropolitan area). The project sought to investigate the performance characteristics of the commonly used Hwalek-Sengstock Elder Abuse Screening Test (HS-EAST), as well as to examine the psychometric properties of a new measure developed to capture culturally salient aspects of mistreatment in American Indian contexts--the Native Elder Life Scale (NELS). Using methods and samples comparable to those in the literature, the HS-EAST performed adequately in these Native samples. The NELS also shows promise for use with this population and assesses different aspects of elder mistreatment than does the HS-EAST.

  11. IGF-independent effects of IGFBP-2 on the human breast cancer cell line Hs578T.

    Science.gov (United States)

    Frommer, Klaus W; Reichenmiller, Katharina; Schutt, Burkhardt S; Hoeflich, Andreas; Ranke, Michael B; Dodt, Gabriele; Elmlinger, Martin W

    2006-08-01

    There is evidence that insulin-like growth factor-binding protein (IGFBP-2), a modulator of the actions of IGFs, also has IGF-independent effects in human tumor cell lines. These involve specific binding of IGFBP-2 to alpha5beta1-integrin, followed by alterations in the phosphorylation status of downstream signaling molecules. Previously, IGFBP-2 has also been shown to be associated with cell proliferation, adhesion and migration. Here, we investigated direct effects of IGFBP-2 on apoptosis and alterations in the expression of related proteins. The breast cancer cell line Hs578T, which shows no IGFBP-2 production of its own and is independent of the IGF-I receptor, was treated with human recombinant IGFBP-2 in order to study the changes in gene expression induced by IGFBP-2. The methods employed for this purpose were oligonucleotide microarrays, real-time RT-PCR, western blotting, and immunoassays. Out of the 440 genes covered by the Oligo GEArray Human Cancer Microarray OHS-802, the expression of 77 genes was directly influenced by IGFBP-2. By the use of real-time quantitative RT-PCR, the gene expression of Nuclear Factor (NF)kappaB, p53, transforming growth factor beta (TGF beta-1), LAMB1 (Laminin, Beta 1), Bcl-2, and IIp45 was found to be significantly upregulated (by 1.2- to 3.05-fold; all P 1.5-fold. By using an ELISA-based and a flow cytometry-based apoptosis assay, IGFBP-2 was found to have a pro-apoptotic effect on Hs578T cells. Our results suggest that IGFBP-2-induced gene expressions are of functional significance for proliferation, cell adhesion, cell migration and apoptosis, and showed that IGFBP-2 can promote apoptosis in tumor cells independent of IGF.

  12. D-Alanine-Controlled Transient Intestinal Mono-Colonization with Non-Laboratory-Adapted Commensal E. coli Strain HS.

    Science.gov (United States)

    Cuenca, Miguelangel; Pfister, Simona P; Buschor, Stefanie; Bayramova, Firuza; Hernandez, Sara B; Cava, Felipe; Kuru, Erkin; Van Nieuwenhze, Michael S; Brun, Yves V; Coelho, Fernanda M; Hapfelmeier, Siegfried

    2016-01-01

    Soon after birth the mammalian gut microbiota forms a permanent and collectively highly resilient consortium. There is currently no robust method for re-deriving an already microbially colonized individual again-germ-free. We previously developed the in vivo growth-incompetent E. coli K-12 strain HA107 that is auxotrophic for the peptidoglycan components D-alanine (D-Ala) and meso-diaminopimelic acid (Dap) and can be used to transiently associate germ-free animals with live bacteria, without permanent loss of germ-free status. Here we describe the translation of this experimental model from the laboratory-adapted E. coli K-12 prototype to the better gut-adapted commensal strain E. coli HS. In this genetic background it was necessary to complete the D-Ala auxotrophy phenotype by additional knockout of the hypothetical third alanine racemase metC. Cells of the resulting fully auxotrophic strain assembled a peptidoglycan cell wall of normal composition, as long as provided with D-Ala and Dap in the medium, but could not proliferate a single time after D-Ala/Dap removal. Yet, unsupplemented bacteria remained active and were able to complete their cell cycle with fully sustained motility until immediately before autolytic death. Also in vivo, the transiently colonizing bacteria retained their ability to stimulate a live-bacteria-specific intestinal Immunoglobulin (Ig)A response. Full D-Ala auxotrophy enabled rapid recovery to again-germ-free status. E. coli HS has emerged from human studies and genomic analyses as a paradigm of benign intestinal commensal E. coli strains. Its reversibly colonizing derivative may provide a versatile research tool for mucosal bacterial conditioning or compound delivery without permanent colonization.

  13. Suppressive effect of CORM-2 on LPS-induced platelet activation by glycoprotein mediated HS1 phosphorylation interference.

    Directory of Open Access Journals (Sweden)

    Dadong Liu

    Full Text Available In recent years, it has been discovered that septic patients display coagulation abnormalities. Platelets play a major role in the coagulation system. Studies have confirmed that carbon monoxide (CO has important cytoprotective and anti-inflammatory function. However, whether CO could alter abnormal activation of platelets and coagulation and thereby reduce the incidence of mortality during sepsis has not been defined. In this report, we have used CO-releasing molecules (CORM-2 to determine whether CO inhibits LPS-induced abnormal activation of platelets and have explored the potential mechanisms. LPS was used to induce activation of platelets in vitro, which were purified from the peripheral venous blood of healthy adult donors. CORM-2 was applied as a potential therapeutic agent. CORM-2 preconditioning and delayed treatment were also studied. We found that in the LPS groups, the function of platelets such as spreading, aggregation, and release were enhanced abnormally. By contrast, the platelets in the CORM-2 group were gently activated. Further studies showed that the expression of platelet membrane glycoproteins increased in the LPS group. Coincidently, both hematopoietic lineage cell-specific protein 1 and its phosphorylated form also increased dramatically. These phenomena were less dramatically seen in the CORM-2 groups. Taken together, we conclude that during LPS stimulation, platelets were abnormally activated, and this functional state may be associated with the signal that is transmitted between membrane glycoproteins and HS1. CORM-released CO suppresses the abnormal activation of platelets by interfering with glycoprotein-mediated HS1 phosphorylation.

  14. Pathomechanism of Insulin Resistance in Men with Central Obesity: Correlation of GGT, GPx, hs-CRP and Plasma Total Cysteine

    Directory of Open Access Journals (Sweden)

    Ritawaty Ritawaty

    2013-08-01

    Full Text Available BACKGROUND: Gamma glutamyltransferase (GGT was reported recently to be associated with inflammation, oxidative stress and increased amino acid. However, role of GGT in insulin resistance pathomechanism is not exactly known. Therefore correlation of GGT with inflammation, oxidative stress and elevated amino acid, in men with central obesity need to be confirmed. METHODS: A cross-sectional study was designed. Men with central obesity were recruited and selected. Anthropometric parameters, creatinine, hs-CRP, fasting glucose, fasting insulin, glutathione peroxidase (GPx activity, GGT, plasma total cysteine (tCys and fatty liver were measured. Subjects were then divided in 4 groups based on waist circumference (WC and fatty liver: Group I: WC ≤100 cm, without fatty liver; Group II: WC ≤100 cm, with fatty liver; Group III: WC >100 cm, without fatty liver; Group IV: WC >100 cm, with fatty liver. All biochemical characteristics in each group were then statistically analyzed. RESULTS: Seventy-two men with central obesity were selected. Numbers of subjects in each group were: Group I: n=33; Group II: n=5; Group III: n=17; Group IV: n=17. We found significant difference of HOMA-IR between Group I and IV, significant correlation between GGT and HOMAIR, and significant negative correlation between tCys with HOMA-IR in Group IV. CONCLUSIONS: GGT was significantly correlated with HOMA-IR in men with WC >100 cm and fatty liver. Further investigation with more subjects is necessary to determine clear GGT cut-off to distinguish subjects with fatty liver and insulin resistance. KEYWORDS: GGT, hs-CRP, GPx, tCys, HOMA-IR, insulin resistance.

  15. Analysis of cardiovascular responses to the H2S donors Na2S and NaHS in the rat.

    Science.gov (United States)

    Yoo, Daniel; Jupiter, Ryan C; Pankey, Edward A; Reddy, Vishwaradh G; Edward, Justin A; Swan, Kevin W; Peak, Taylor C; Mostany, Ricardo; Kadowitz, Philip J

    2015-08-15

    Hydrogen sulfide (H2S) is an endogenous gaseous molecule formed from L-cysteine in vascular tissue. In the present study, cardiovascular responses to the H2S donors Na2S and NaHS were investigated in the anesthetized rat. The intravenous injections of Na2S and NaHS 0.03-0.5 mg/kg produced dose-related decreases in systemic arterial pressure and heart rate, and at higher doses decreases in cardiac output, pulmonary arterial pressure, and systemic vascular resistance. H2S infusion studies show that decreases in systemic arterial pressure, heart rate, cardiac output, and systemic vascular resistance are well-maintained, and responses to Na2S are reversible. Decreases in heart rate were not blocked by atropine, suggesting that the bradycardia was independent of parasympathetic activation and was mediated by an effect on the sinus node. The decreases in systemic arterial pressure were not attenuated by hexamethonium, glybenclamide, N(w)-nitro-L-arginine methyl ester hydrochloride, sodium meclofenamate, ODQ, miconazole, 5-hydroxydecanoate, or tetraethylammonium, suggesting that ATP-sensitive potassium channels, nitric oxide, arachidonic acid metabolites, cyclic GMP, p450 epoxygenase metabolites, or large conductance calcium-activated potassium channels are not involved in mediating hypotensive responses to the H2S donors in the rat and that responses are not centrally mediated. The present data indicate that decreases in systemic arterial pressure in response to the H2S donors can be mediated by decreases in vascular resistance and cardiac output and that the donors have an effect on the sinus node independent of the parasympathetic system. The present data indicate that the mechanism of the peripherally mediated hypotensive response to the H2S donors is uncertain in the intact rat. Copyright © 2015 the American Physiological Society.

  16. Anti-tumor activity of a novel HS-mimetic-vascular endothelial growth factor binding small molecule.

    Directory of Open Access Journals (Sweden)

    Kazuyuki Sugahara

    Full Text Available The angiogenic process is controlled by variety of factors of which the vascular endothelial growth factor (VEGF pathway plays a major role. A series of heparan sulfate mimetic small molecules targeting VEGF/VEGFR pathway has been synthesized. Among them, compound 8 (2-butyl-5-chloro-3-(4-nitro-benzyl-3H-imidazole-4-carbaldehyde was identified as a significant binding molecule for the heparin-binding domain of VEGF, determined by high-throughput-surface plasmon resonance assay. The data predicted strong binding of compound 8 with VEGF which may prevent the binding of VEGF to its receptor. We compared the structure of compound 8 with heparan sulfate (HS, which have in common the functional ionic groups such as sulfate, nitro and carbaldehyde that can be located in similar positions of the disaccharide structure of HS. Molecular docking studies predicted that compound 8 binds at the heparin binding domain of VEGF through strong hydrogen bonding with Lys-30 and Gln-20 amino acid residues, and consistent with the prediction, compound 8 inhibited binding of VEGF to immobilized heparin. In vitro studies showed that compound 8 inhibits the VEGF-induced proliferation migration and tube formation of mouse vascular endothelial cells, and finally the invasion of a murine osteosarcoma cell line (LM8G7 which secrets high levels of VEGF. In vivo, these effects produce significant decrease of tumor burden in an experimental model of liver metastasis. Collectively, these data indicate that compound 8 may prevent tumor growth through a direct effect on tumor cell proliferation and by inhibition of endothelial cell migration and angiogenesis mediated by VEGF. In conclusion, compound 8 may normalize the tumor vasculature and microenvironment in tumors probably by inhibiting the binding of VEGF to its receptor.

  17. D-Alanine-Controlled Transient Intestinal Mono-Colonization with Non-Laboratory-Adapted Commensal E. coli Strain HS.

    Directory of Open Access Journals (Sweden)

    Miguelangel Cuenca

    Full Text Available Soon after birth the mammalian gut microbiota forms a permanent and collectively highly resilient consortium. There is currently no robust method for re-deriving an already microbially colonized individual again-germ-free. We previously developed the in vivo growth-incompetent E. coli K-12 strain HA107 that is auxotrophic for the peptidoglycan components D-alanine (D-Ala and meso-diaminopimelic acid (Dap and can be used to transiently associate germ-free animals with live bacteria, without permanent loss of germ-free status. Here we describe the translation of this experimental model from the laboratory-adapted E. coli K-12 prototype to the better gut-adapted commensal strain E. coli HS. In this genetic background it was necessary to complete the D-Ala auxotrophy phenotype by additional knockout of the hypothetical third alanine racemase metC. Cells of the resulting fully auxotrophic strain assembled a peptidoglycan cell wall of normal composition, as long as provided with D-Ala and Dap in the medium, but could not proliferate a single time after D-Ala/Dap removal. Yet, unsupplemented bacteria remained active and were able to complete their cell cycle with fully sustained motility until immediately before autolytic death. Also in vivo, the transiently colonizing bacteria retained their ability to stimulate a live-bacteria-specific intestinal Immunoglobulin (IgA response. Full D-Ala auxotrophy enabled rapid recovery to again-germ-free status. E. coli HS has emerged from human studies and genomic analyses as a paradigm of benign intestinal commensal E. coli strains. Its reversibly colonizing derivative may provide a versatile research tool for mucosal bacterial conditioning or compound delivery without permanent colonization.

  18. The Value of HbA1c and hs-CRP Combined Detection in Diagnosis of Diabetic Retinopathy%HbA1c、hs-CRP 联合检测诊断糖尿病视网膜病变的价值

    Institute of Scientific and Technical Information of China (English)

    李孝才; 孙学芬; 吴建霞; 马雪燕

    2016-01-01

    目的:探讨糖化血红蛋白(HbA1c)和高敏 C 反应蛋白(hs-CRP)联合检测诊断糖尿病视网膜病变的价值。方法:选择正常体检者(A 组)、糖尿病无视网膜病变者(B 组)、糖尿病视网膜病变者(C 组)各72例,采用离子交换液相层析法和胶乳增强免疫比浊法测定 HbA1c 及 hs-CRP。结果:无视网膜病变组和视网膜病变组的 HbA1c 和 hs-CRP(mg/L)含量均比健康对照组高,具有统计学意义(P0.05);但是 HbA1c +Hs-CRP 诊断糖尿病视网膜病变的特异为90.3%,分别与 HbA1c 和 hs-CRP 存在明显差异(P 0.05); but specific to the HbA1c of hs CRP in the diagnosis of diabetic retinopathy was 90.3%, respectively and hs CRP and HbA1c exist significant difference (P< 0.05). Conclusion:The detection of HBA1c and Hs-CRPis helpful to early detection of diabetic retinopathy, which can be used asmonitoring indicators for progress and treatment effect in type 2 diabetes mellitus patients.

  19. 急性颅脑损伤患者血清NSE、hs-CRP和S100B水平变化的临床意义

    Institute of Scientific and Technical Information of China (English)

    闫桂秀

    2016-01-01

    目的:探讨血清NSE、hs-CRP和S100B水平在急性颅脑损伤患者治疗前与治疗后的对比变化及其临床意义.方法:分别采用放射免疫分析法(RIA)、免疫比浊法(Immuno-turbidimetry)和酶联法(ELISA)检测血清NSE、hs-CRP和S100B,检测了急性颅脑损伤患者(32例)治疗前与治疗后血清NSE、hs-CRP和S100B的水平,和健康人组(35例)相关水平比较.结果:血清NSE、hs-CRP和S100B水平在急性颅脑损伤患者中,治疗前显著高于正常人组,两者有显著性的差异性(P<0.01),经二周治疗后恢复与正常人组相当,两者无显著性差异(P>0.05),血清NSE水平与hs-CRP、S100B呈显著正相关(r=0.5412 0.6018 P<0.01).结论:检测血清NSE、hs-CRP和S100B水平的变化有助于估计急性颅脑损伤患者的病情轻重和预后.

  20. Effect of red yeast rice combined with antioxidants on lipid pattern, hs-CRP level, and endothelial function in moderately hypercholesterolemic subjects.

    Science.gov (United States)

    Cicero, Arrigo F G; Morbini, Martino; Parini, Angelo; Urso, Riccardo; Rosticci, Martina; Grandi, Elisa; Borghi, Claudio

    2016-01-01

    Our aim was to test, through a crossover, double-blind, placebo-controlled randomized clinical trial, if a short-term treatment with 10 mg monacolins combined with antioxidants could improve lipid pattern, high-sensitivity C-reactive protein (hs-CRP), and endothelial function in a small cohort of moderately hypercholesterolemic subjects. Thus, 25 healthy, moderately hypercholesterolemic subjects were consecutively enrolled and, after 4 weeks of stabilization diet, were randomized to the sequence placebo followed by a washout, monacolins or monacolins followed by a washout, placebo, with each period being 4 weeks long. At each study step, a complete lipid pattern, safety parameters, hs-CRP, and endothelial function have been measured. When compared to the placebo phase, during monacolin treatment, patients experienced a more favorable percentage change in total cholesterol (TC) (TC after monacolin treatment, -18.35%; TC after placebo treatment, -5.39%), low-density lipoprotein cholesterol (LDL-C) (LDL after monacolin treatment, -22.36%; LDL after placebo treatment, -1.38%), non-high-density lipoprotein cholesterol (HDL-C) (non-HDL after monacolin treatment, -22.83%; non-HDL after placebo treatment: -7.15%), hs-CRP (hs-CRP after monacolin treatment: -2.33%; hs-CRP after placebo treatment, 2.11%), and endothelial function (pulse volume displacement after monacolin treatment, 18.59%; pulse volume displacement after placebo treatment, -6.69%). No significant difference was observed with regard to triglycerides, HDL-cholesterol, and safety parameters. On the basis of our data, we could demonstrate that a 10 mg monacolin nutraceutical treatment appears to safely reduce cholesterolemia, hs-CRP, and markers of vascular remodeling in moderately hypercholesterolemic subjects. These results need to be confirmed in larger patient samples and in studies with longer duration.

  1. A Comparison of hs-CRP Levels in New Diabetes Groups Diagnosed Based on FPG, 2-hPG, or HbA1c Criteria.

    Science.gov (United States)

    Tutuncu, Yildiz; Satman, Ilhan; Celik, Selda; Dinccag, Nevin; Karsidag, Kubilay; Telci, Aysegul; Genc, Sema; Issever, Halim; Tuomilehto, Jaakko; Omer, Beyhan

    2016-01-01

    Fasting plasma glucose (FPG) and hemoglobin A1c (HbA1c) have been used to diagnose new-onset diabetes mellitus (DM) in order to simplify the diagnostic tests compared with the 2-hour oral glucose tolerance test (OGTT; 2-hPG). We aimed to identify optimal cut-off points of high sensitive C-reactive protein (hs-CRP) in new-onset DM people based on FPG, 2-hPG, or HbA1c methods. Data derived from recent population-based survey in Turkey (TURDEP-II). The study included 26,499 adult people (63% women, response rate 85%). The mean serum concentration of hs-CRP in women was higher than in men (p hs-CRP level than FPG based and 2-hPG based DM cases. In HbA1c, 2-hPG, and FPG based new-onset DM people, cut-off levels of hs-CRP in women were 2.9, 2.1, and 2.5 mg/L [27.5, 19.7, and 23.5 nmol/L] and corresponding values in men were 2.0, 1.8, and 1.8 mg/L (19.0, 16.9, and 16.9 nmol/L), respectively (sensitivity 60-65% and specificity 54-64%). Our results revealed that hs-CRP may not further strengthen the diagnosis of new-onset DM. Nevertheless, the highest hs-CRP level observed in new-onset DM people diagnosed with HbA1c criterion supports the general assumption that this method might recognize people in more advanced diabetic stage compared with other diagnostic methods.

  2. Clinical Significance of Determination of Serum Hypersensitive C-reactive Protein (hs-CRP) and E-Selectin Levels in Patients with Coronary Heart Diseases%冠心病患者血清hs-CRP和E-Selectin检测的临床意义

    Institute of Scientific and Technical Information of China (English)

    杨春秀

    2007-01-01

    目的:探讨了冠心病患者血清超敏C反应蛋白(hs-CRP)和E-选择素(E-Selectin)水平的变化及意义.方法:应用免疫比浊法检测hs-CRP,ELISA法检测E-Selectin水平对58例冠心病患者进行了血清hs-CRP,E-Selectin水平检测.其中稳定型心绞痛25例,不稳定型心绞痛20例,急性心肌梗死13例,并以35名正常健康人作比较.结果:冠心病患者血清hs-CRP和E-Selectin水平明显高于正常人组(P<0.01),急性心肌梗死组和不稳定型心绞痛组有明显高于稳定型心绞痛组(P<0.01),冠心病组血清hs-CRP、E-Selectin水平与冠状动脉狭窄程度无明显相关性(P>0.05).结论:血清hs-CRP和E-Selectin水平的变化与冠心病的发生、发展有关,但与冠状动脉狭窄程度无关.

  3. JUPITER to Earth: A statin helps people with normal LDL-C and high hs-CRP, but what does it mean?

    OpenAIRE

    2009-01-01

    The JUPITER trial (Justification for the Use of Statins in Prevention: an Intervention Trial Evaluating Rosuvastatin) (N Engl J Med 2008; 359:2195–2207) compared rosuvastatin (Crestor) 20 mg daily vs placebo in apparently healthy people who had levels of low-density lipoprotein cholesterol (LDL-C) lower than 130 mg/dL but elevated levels (≥ 2 mg/L) of high-sensitivity C-reactive protein (hs-CRP). Rosuvastatin treatment lowered LDL-C levels by 50% and hs-CRP levels by 37%, accompanied by a 44%...

  4. The influence of statin treatment on the inflammatory biomarkers YKL-40 and HsCRP in patients with stable coronary artery disease

    DEFF Research Database (Denmark)

    Mygind, Naja Dam; Harutyunyan, Marina J; Mathiasen, Anders Bruun;

    2011-01-01

    OBJECTIVE: The inflammatory biomarker YKL-40 is elevated and associated with mortality in patients with stable coronary artery disease (CAD). The aim was to investigate the influence of statin treatment and lipid status on serum YKL-40 and Hs-CRP in patients with stable CAD. DESIGN: Serum YKL-40......, HsCRP, total cholesterol, HDL-c, LDL-c and triglycerides levels were measured in 404 statin treated and in 404 matched non-statin treated patients with stable CAD. RESULTS: YKL-40 was significantly higher in non-statin treated 110 µg/l (median) compared with 65 µg/l in statin treated (p ...

  5. Heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3B1 (HS3ST3B1) promotes angiogenesis and proliferation by induction of VEGF in acute myeloid leukemia cells.

    Science.gov (United States)

    Zhang, Lei; Song, Kai; Zhou, Ling; Xie, Zhishen; Zhou, Ping; Zhao, Yiming; Han, Yue; Xu, Xiaojun; Li, Ping

    2015-06-01

    Heparan sulfate (HS) are complex polysaccharides that reside on the plasma membrane of almost all mammalian cells, and play an important role in physiological and pathological conditions. Heparan sulfate D-glucosamine 3-O-sulfotransferase 3B1 (HS3ST3B1) participates in the last biosynthetic steps of HS and transfers sulfate to the 3-O-position of glucosamine residues to yield mature sugar chains. To date very few biological processes or proteins have been described that are modulated by HS3ST3B1. In this study, we observed that HS3ST3B1 positively contributed to acute myeloid leukemia (AML) progression in vitro and in vivo, and these activities were associated with an induction of the proangiogenic factor VEGF expression and shedding. Moreover, the effects of HS3ST3B1 on VEGF release can be attenuated after treatment of heparanase inhibitor suramin, which prevented VEGF secretion and subsequently blocked VEGF-induced activation of ERK and AKT, suggesting that 3-O-sulfation of HS by HS3ST3B1 facilitated VEGF shedding; the effects of HS3ST3B1 on activation of ERK and AKT can also be blocked by VEGFR inhibitor axitinib, suggestive of a relationship between 3-O-sulfation of HS and VEGF-activated signaling pathways. Taken together, our findings support that VEGF is an important functional target of HS3ST3B1 and provide a new mechanism of HS3ST3B1 in AML.

  6. A risk assessment on primary level in hs-cTnT level no more than 14 ng/L in the onset of acute myocar-dial infarction in patients with chest pain%胸痛患者初次hs-cTnT水平≤14 ng/L发生急性心肌梗死的危险性评价

    Institute of Scientific and Technical Information of China (English)

    郭英; 黄华兰; 朱帅; 李贵星

    2016-01-01

    目的:研究胸痛患者初次hs-cTnT水平≤14 ng/L时发生急性心肌梗死(AMI)的危险性。方法:纳入2012年1月至2013年12月因胸痛就诊于四川大学华西医院急诊科患者3096例,根据初次hs-cTnT水平、心电图将患者分为hs-cTnT≤14 ng/L且心电图提示无缺血表现组和hs-cTnT >14 ng/L组,计算30 d内两组发生心肌梗死和死亡的危险度及阴性预测值。结果:30 d 内hs-cTnT≤14 ng/L 组发生AMI 37例,绝对危险度为2.35(1.86~2.74),30 d 内有4例患者死亡,绝对危险度为0.29(0.12~0.53);初次 hs-cTnT水平≤14 ng/L 且心电图提示无缺血表现组发生 AMI 9例,绝对危险度为0.58(0.42~0.74),30 d 内无患者死亡。结论:胸痛患者初次hs-cTnT水平≤14 ng/L 且心电图提示无缺血表现可基本排除AMI,阴性预测值为99.6%,准确性高,动态监测5 h hs-cTnT≤14 ng/L直接排除AMI。%Objective To investigate the association of chest pain patients with primary level in high-sensitivity troponin T (hs-cTnT) level no more than 14 ng/L in the onset of acute myocardial infarction in pa-tients with chest pain. Methods We enrolled 3 096 participants from January 2012 to December 2013 in West China Hospital, Sichuan University. All patients were classified two groups (hs-cTnT > 14 ng/L, hs-cTnT ≤14 ng/L and no ischemia on ECG) according to hs-cTnT levels and ECG. We evaluated the risk of myocardial in-farction and death and negative predictive value in 30 days. Results Thirty-seven patients were diagnosed in having acute myocardial infarction (AMI) and 4 patients were dead in the hs-cTnT > 14 ng/L group in 30 days in the absolute risk 2.35(1.86-2.74) and 0.29(0.12-0.53); 9 patients were diagnosed as having AMI and no patients were dead in the hs-cTnT ≤ 14 ng/L group in 30 days in the absolute risk 0.58 (0.42-0.74). Conclu-sion Chest pain patients whose primary levels no more than 14

  7. 子痫前期患者血清MPO、hs-CRP水平与新生儿出生体重的相关性%Relatingship between serum myeloperoxidase,hs-CRP levels and neonatal birth weight in patients with preeclampsia

    Institute of Scientific and Technical Information of China (English)

    罗金英; 颜建英

    2014-01-01

    目的:研究重度子痫前期( PE )患者血清髓过氧化物酶( MPO )及超敏C反应蛋白( hs-CRP)水平与新生儿出生体重的关系。方法:选择2012年1~10月于我院产科住院分娩的60例重度PE孕妇,按发病时孕周不同分为早发型PE组(发病时孕周0.05)。(2)早发型与晚发型PE组血清hs-CRP高于同期对照组,差异均有统计学意义(P0.05)。(3)早发型与晚发型PE组的血清MPO、hs-CRP水平与新生儿出生体重均呈负相关(P0.05)。结论:血清MPO、hs-CRP可能在PE的病理生理过程中发挥作用。重度PE患者血清MPO、hs-CRP水平升高可引起新生儿体重下降,其机制可能与胎儿宫内生长发育有关。%Objective:To investigate the relationships between concentrations of serum MPO,hs-CRP levels birth weight in patients with preeclampsia. Methods:A total of 60 women with PE and 60 normal pregnant women as control participated in this study. Patients with PE were divided into early-onset group (n=30,presented at0. 05). (2)Serum hs-CRP level of early-onset and late-onset PE group were higher than control group1 and control group 2,the differences were statistically significant (P0. 05). (3)Serum MPO,hs-CRP level and neonatal birth weight of early-onset and late-onset PE group were negatively correlated ( P0. 05). Conclusion:Serum MPO,hs-CRP levels of preeclampsia patients suggesting that they may be involved in the pathophysiologi-cal process of preeclampsia. In severe preeclampsia patients,elevated serum MPO,hs-CRP lev-els can cause low neonatal weight,and may be associated with fetal growth.

  8. Pilates versus resistance exercise on the serum levels of hs-CRP, in the abdominal circumference and body mass index (BMI in elderly individuals

    Directory of Open Access Journals (Sweden)

    Maria Adesilda Silva Pestana

    2016-06-01

    Full Text Available Recent studies have shown that the elderly exhibit a subclinical state of inflammation associated with increased adipose tissue and several comorbidities. To compare the effects of mat Pilates based exercises and resistance exercise on the serum levels of reactive C protein of high sensitivity (hs-CRP, in the abdominal circumference (AC and the body mass index (BMI in the elderly. It is a randomised clinical trial with a sample of 78 elderly individuals (median age 69 years. The active independent variable investigated was nature of treatment intervention (Pilates mat based exercises vs resistance exercise, and the dependent variables were hs-CRP level, AC and BMI. The statistical analysis used Wilcoxon signed rank and Mann-Whitney tests. The correlation between the continuous variables was assessed using Spearman’s coefficient of correlation. The data were analysed using SPSS software version 17.0, and probability values lower than 5% (p< 0.05 were considered statistically significant. Mat Pilates based exercises exhibited reductions in serum hs-CPR level (Wilcoxon signed rank test; z = -2.466, p = 0.01, BMI (Wilcoxon signed rank test; z = -3.295, p = 0.001 and AC (Wilcoxon signed rank test; z = -3.398, p = 0.01. Mat Pilates based exercises promoted a significant reduction of the serum hs-CRP levels and anthropometric measurements in elderly individuals.

  9. [Fe(II)LSCo(III)LS]2 ⇔ [Fe(III)LSCo(II)HS]2 photoinduced conversion in a cyanide-bridged heterobimetallic molecular square.

    Science.gov (United States)

    Mercurol, Julie; Li, Yanling; Pardo, Emilio; Risset, Olivia; Seuleiman, Mannan; Rousselière, Hélène; Lescouëzec, Rodrigue; Julve, Miguel

    2010-12-21

    The self-assembly of [Fe(III){B(pz)(4)}(CN)(3)](-) and [Co(II)(bik)(2)(S)(2)](2+) affords the diamagnetic cyanide-bridged [Fe(II)(LS)Co(III)(LS)](2) molecular square which is converted into the corresponding magnetic [Fe(III)(LS)Co(II)(HS)](2) species under light irradiation at relatively low temperatures.

  10. Abrupt two-step and symmetry breaking spin crossover in an iron(III) complex: an exceptionally wide [LS-HS] plateau.

    Science.gov (United States)

    Harding, David J; Phonsri, Wasinee; Harding, Phimphaka; Murray, Keith S; Moubaraki, Boujemaa; Jameson, Guy N L

    2015-09-14

    [Fe(qsal-Br)2]NO3·2MeOH is reported which undergoes abrupt two step symmetry breaking spin crossover, T½(1st step) = 136 K and T½(2nd step) = 232 K with a hysteresis of 16 K and 5 K, respectively, and an unprecedented [HS-LS] plateau of 96 K.

  11. A stage—specific protein factor binding to a CACCC motif in both human β—globin gene promoter and 5‘—HS2 region

    Institute of Scientific and Technical Information of China (English)

    SUNTONG; YADICHEN; 等

    1994-01-01

    The DNaseI hypersensitive site 2 (HS2) of human β-globin locus control region(LCR) is required for the high level expression of human β-globin genes.In the present study,a stage-specific protein factor (LPF-β) was identified in the nuclear extract prepared from mouse fetal liver at d 18 of gestation,which could bind to the HS2 region of human β-globin LCR.We also found that the shift band of LPF-β factor could be competed by human β-globin promoter.However,it couldn't be competed by human ε-globin promoter or by human Aγ-globin promoter.Furthermore,our data demonstrated that the binding-sequence of LPF-β factor is 5'CACACCCTA 3',which is located at the HS2 region of β-LCR(from-10845 to-10853 bp)and human β-globin promoter(from-92 to -84 bp).We speculated that these regions containing the CACCC box in both the human β-globin promoter and HS2 might function as stage selector elements in the regulation of human β-globin switching and the LPF-β factor might be a stage-specific protein factor involved in the regulation of human β-globin gene expression.

  12. Simulations of Hurricane Nadine (2012) during HS3 Using the NASA Unified WRF with Aerosol-Cloud Microphysics-Radiation Coupling

    Science.gov (United States)

    Shi, J. J.; Braun, S. A.; Sippel, J. A.; Tao, W. K.; Tao, Z.

    2014-12-01

    The impact of the SAL on the development and intensification of hurricanes has garnered significant attention in recent years. Many past studies have shown that synoptic outbreaks of Saharan dust, which usually occur from late spring to early fall and can extend from western Africa across the Atlantic Ocean into the Caribbean, can have impacts on hurricane genesis and subsequent intensity change. The Hurricane and Severe Storm Sentinel (HS3) mission is a multiyear NASA field campaign with the goal of improving understanding of hurricane formation and intensity change. One of HS3's primary science goals is to obtain measurements to help determine the extent to which the Saharan air layer impacts storm intensification. HS3 uses two of NASA's unmanned Global Hawk aircrafts equipped with three instruments each to measure characteristics of the storm environment and inner core. The Goddard microphysics and longwave/shortwave schemes in the NASA Unified Weather Research and Forecasting (NU-WRF) model have been coupled in real-time with the Goddard Chemistry Aerosol Radiation and Transport (GOCART) model in WRF-Chem to account for the direct (radiation) and indirect (microphysics) impact. NU-WRF with interactive aerosol-cloud-radiation physics is used to generate 30-member ensemble simulations of Nadine (2012) with and without the aerosol interactions. Preliminary conclusions related to the impact of the SAL on the evolution of Nadine from the HS3 observations and model output will be described.

  13. Increased carotid intima-media thickness associated with high hs-CRP levels is a predictor of unstable coronary artery disease.

    Science.gov (United States)

    Abhashi, Sejran Ahmet; Kryeziu, Fadil Uke; Nazreku, Feim Durak

    2013-08-01

    Increased values of carotid intima-media thickness (CIMT) and high-sensitivity C-reactive protein (hs-CRP) are predictors of acute coronary events. We analysed the link between CIMT and hs-CRP in cases with coronary artery disease (CAD). From 1 January to 30 June 2012, we evaluated 43 patients with acute coronary syndrome (group A), 50 patients with stable coronary artery disease (group B) and 50 healthy volunteers (group C). All were analysed for CIMT and hs-CRP levels. CIMT values were higher in groups A and B (0.94 ± 0.21 mm, 0.89 ± 0.19 mm, respectively) and lower in group C (0.64 ± 0.09 mm), and this was statistically significant (p hs-CRP were higher in group A (1.87 ± 0.36 mg/l) and lower in groups B and C (1.07 ± 0.28 mg/l, 0.97 ± 0.45 mg/l, respectively) and this was also statistically significant (p < 0.0001).

  14. Efficacy and tolerability of a combined lipid-lowering nutraceutical on cholesterolemia, hs-CRP level and endothelial function in moderately hypercholesterolemic subjects.

    Science.gov (United States)

    Cicero, A F; Colletti, A; Rosticci, M; Grandi, E; Borghi, C

    2016-01-01

    Our aim was to test, by a double-blind, placebo-controlled randomized clinical trial, whether a short-term treatment with a combined lipid-lowering nutraceutical could improve endothelial function in a cohort of moderately hypercholesterolemic subjects. Thus, 80 healthy, moderately hypercholesterolemic subjects were consecutively enrolled and, after 4 weeks of stabilization diet, they were randomized to either the tested lipid-lowering nutraceutical or placebo for 8 weeks. At the beginning and end of treatment a complete lipid pattern, safety parameters, hs-CRP and endothelial function were measured. When compared to placebo, during nutraceutical treatment patients experienced a more favorable percentage change in total cholesterol (TC vs baseline: -17.9%; TC vs placebo: -5.6%), LDL-cholesterol (LDL-C vs baseline: -23.3%; LDL-C vs placebo: -2.8%), hs-CRP (hs-CRP vs baseline: -2.4%; hs-CRP vs placebo: -1.5%), and endothelial function (pulse volume displacement vs baseline: +17%; pulse volume displacement vs placebo treatment: -3.3%). No significant difference was observed in respect to effects on triglycerides, HDL-cholesterol and safety parameters. On the basis of our data, the tested lipid-lowering nutraceutical seems to significantly improve endothelial function in moderately hypercholesterolemic subjects. These results have to be confirmed on larger patient samples and over longer periods.

  15. Elevated levels of Hs-CRP and IL-6 after delivery are associated with depression during the 6 months post partum.

    Science.gov (United States)

    Liu, Hao; Zhang, Yang; Gao, Yutao; Zhang, Zhenyu

    2016-09-30

    The objective of this study is to determine whether inflammatory markers (high-sensitivity C-reactive protein (Hs-CRP) and interleukin (IL)-6) early in the postpartum period contribute to the development of postpartum depression (PPD). From 4 May 2014 to 30 June 2014, all eligible women not on medication for depression giving birth at the Beijing Chao-Yang hospital were consecutively recruited and followed up for 6 months. Depression symptoms were measured with the Edinburgh Postnatal Depression Scale (EPDS), and inflammatory biomarkers (Hs-CRP and IL-6) were tested. During the study period, 296 women were enrolled and completed follow-up. In these women, 45 (15.2%) were considered as meeting the criteria for PPD. Serum levels of Hs-CRP and IL-6 in women with PPD were significantly higher than those without PPD (all PHs-CRP (0.837 (95% CI, 0.781-0.894), PHs-CRP were independent predictors of PPD. The present study demonstrates a strong relationship between elevated serum Hs-CRP and IL-6 levels at admission and the development of PPD within 6 months.

  16. Serum hs-CRP varies with dietary cholesterol, but not dietary fatty acid intake in individuals free of any history of cardiovascular disease.

    Science.gov (United States)

    Mazidi, M; Heidari-Bakavoli, A; Khayyatzadeh, S S; Azarpazhooh, M R; Nematy, M; Safarian, M; Esmaeili, H; Parizadeh, S M R; Ghayour-Mobarhan, M; Kengne, A P; Ferns, G A

    2016-12-01

    The objective of this study was to investigate whether serum high-sensitivity C-reactive protein (hs-CRP) concentration varies with dietary fatty acid intake in Iranian adults free of any history of cardiovascular disease (CVD). This cross-sectional study involved 8105 adults (3142 men) aged 35-65 years. Dietary intake was assessed using 24-h dietary recalls. The relationship between anthropometric, cardiometabolic risk factors and dietary data and serum hs-CRP was assessed using SPSS software. Median crude dietary saturated fat decreased across hs-CRP quarters (P =0.009 for linear trend), whereas energy-adjusted total fat (P =0.017), trans-fat (P =0.016), monounsaturated fatty acids (P =0.030) and cholesterol (P =0.005) monotonically increased, with some evidence of statistical interactions by gender. In conclusion, serum hs-CRP concentrations were associated with some components of dietary fatty acid intake in our population of individuals without CVD, suggesting that dietary fat intake could be associated with subclinical inflammation.

  17. The Effect of High-Dose Vitamin D3 on Soluble P-Selectin and hs-CRP Level in Patients With Venous Thromboembolism: A Randomized Clinical Trial.

    Science.gov (United States)

    Gholami, Kheirollah; Talasaz, Azita Hajhossein; Entezari-Maleki, Taher; Salarifar, Mojtaba; Hadjibabaie, Molouk; Javadi, Mohammad Reza; Dousti, Samaneh; Hamishehkar, Hadi; Maleki, Saleh

    2016-07-01

    High plasma level of P-selectin is associated with the development of venous thromboembolism (VTE). Furthermore, supplementation of vitamin D could decrease thrombotic events. Hence, this study was designed to examine whether the administration of vitamin D can influence the plasma level of P-selectin in patients with VTE. In the randomized controlled trial, 60 patients with confirmed acute deep vein thrombosis and/or pulmonary embolism (PE) were randomized into the intervention (n = 20) and control (n = 40) groups. The intervention arm was given an intramuscular single dose of 300 000 IU vitamin D3 Plasma level of 25-hydroxy vitamin D, P-selectin, and high-sensitive C-reactive protein (hs-CRP) was measured at baseline and 4 weeks after. The plasma level of P-selectin (95% confidence interval = -5.99 to -1.63, P = .022) and hs-CRP (P = .024) significantly declined in vitamin D-treated group, while only hs-CRP was significantly decreased in the control group (P = .011). However, the magnitude of these reductions was not statistically significant. This study could not support the potential benefit of the high-dose vitamin D on plasma level of P-selectin and hs-CRP in patients with VTE.

  18. Evaluation of Hs-CRP levels and interleukin 18 (-137G/C) promoter polymorphism in risk prediction of coronary artery disease in first degree relatives.

    Science.gov (United States)

    G, Rajesh Kumar; K, Mrudula Spurthi; G, Kishore Kumar; Kurapati, Mohanalatha; M, Saraswati; T, Mohini Aiyengar; P, Chiranjeevi; G, Srilatha Reddy; S, Nivas; P, Kaushik; K, Sanjib Sahu; H, Surekha Rani

    2015-01-01

    Coronary Artery Disease (CAD) is clearly a multifactorial disease that develops from childhood and ultimately leads to death. Several reports revealed having a First Degree Relatives (FDRS) with premature CAD is a significant autonomous risk factor for CAD development. C - reactive protein (CRP) is a member of the pentraxin family and is the most widely studied proinflammatory biomarker. IL-18 is a pleiotrophic and proinflammatory cytokine which is produced mainly by macrophages and plays an important role in the inflammatory cascade. Hs-CRP levels were estimated by ELISA and Genotyping of IL-18 gene variant located on promoter -137 (G/C) by Allele specific PCR in blood samples of 300 CAD patients and 300 controls and 100 FDRS. Promoter Binding sites and Protein interacting partners were identified by Alibaba 2.1 and Genemania online tools respectively. Hs-CRP levels were significantly high in CAD patients followed by FDRS when compared to controls. In IL-18 -137 (G/C) polymorphism homozygous GG is significantly associated with occurrence of CAD and Hs-CRP levels were significantly higher in GG genotype subjects when compared to GC and CC. IL-18 was found to be interacting with 100 protein interactants. Our results indicate that Hs-CRP levels and IL-18-137(G/C) polymorphism may help to identify risk of future events of CAD in asymptomatic healthy FDRS.

  19. Evaluation of Hs-CRP levels and interleukin 18 (-137G/C promoter polymorphism in risk prediction of coronary artery disease in first degree relatives.

    Directory of Open Access Journals (Sweden)

    Rajesh Kumar G

    Full Text Available Coronary Artery Disease (CAD is clearly a multifactorial disease that develops from childhood and ultimately leads to death. Several reports revealed having a First Degree Relatives (FDRS with premature CAD is a significant autonomous risk factor for CAD development. C - reactive protein (CRP is a member of the pentraxin family and is the most widely studied proinflammatory biomarker. IL-18 is a pleiotrophic and proinflammatory cytokine which is produced mainly by macrophages and plays an important role in the inflammatory cascade.Hs-CRP levels were estimated by ELISA and Genotyping of IL-18 gene variant located on promoter -137 (G/C by Allele specific PCR in blood samples of 300 CAD patients and 300 controls and 100 FDRS. Promoter Binding sites and Protein interacting partners were identified by Alibaba 2.1 and Genemania online tools respectively. Hs-CRP levels were significantly high in CAD patients followed by FDRS when compared to controls. In IL-18 -137 (G/C polymorphism homozygous GG is significantly associated with occurrence of CAD and Hs-CRP levels were significantly higher in GG genotype subjects when compared to GC and CC. IL-18 was found to be interacting with 100 protein interactants.Our results indicate that Hs-CRP levels and IL-18-137(G/C polymorphism may help to identify risk of future events of CAD in asymptomatic healthy FDRS.

  20. Effects of insulin therapy on weight gain and fat distribution in the HF/HS-STZ rat model of type 2 diabetes.

    Science.gov (United States)

    Skovsø, S; Damgaard, J; Fels, J J; Olsen, G S; Wolf, X A; Rolin, B; Holst, J J

    2015-10-01

    Insulin therapy is required for many patients with the obesity-related disorder type 2 diabetes, but is also associated with weight gain. The specific location of adipose tissue location matters to cardiovascular disease (CVD) risk. We investigated effects of exogenous insulin on fat distribution in the high-fat/high-sucrose fed rat treated with streptozotocin (HF/HS-STZ) rat model of type 2 diabetes. We also examined effects of insulin therapy on circulating CVD markers, including adiponectin, triglycerides (TGs), total cholesterol and high-density lipoprotein. Male SD rats were HF/HS fed for 5 weeks followed by STZ treatment to mimic the hallmarks of human obesity-associated insulin resistance followed by hyperglycemia. Magnetic resonance imaging and computed tomography were used to determine total fat, abdominal fat distribution and liver fat before and after insulin therapy in HF/HS-STZ rats. HbA1c%, TGs, cholesterol, high-density lipoprotein and adiponectin were analyzed by conventional methods adapted for rats. Insulin therapy lowered HbA1c (Pfat mass (Ptissue as compared with visceral adipose tissue (Pfat were observed after insulin therapy, whereas plasma TG and cholesterol levels were decreased (Pinsulin therapy modulates fat distribution. Specifically, our data show that insulin has a relatively positive effect on CVD-associated parameters, including abdominal fat distribution, lean body mass, adiponectin, TGs and HDL in HF/HS-STZ rats, despite a modest gain in weight.

  1. Effect of levosimendan on heart function and hs-CRP, IL-6, TNF-α levels in elderly patients with acute myocardial infarction complicated heart failure

    Institute of Scientific and Technical Information of China (English)

    Wei-Peng Song; Qiang-Hua Guo; Hong-Dan Jia; Ting-Ting Song; Li Liu

    2016-01-01

    Objective:To observe the effect of levosimendan on heart function and hs-CRP, IL-6, TNF-α levels in elderly patients with acute myocardial infarction complicated heart failure. Methods:A total of80 elderly patients with acute myocardial infarction complicated heart failure were randomly divided into control group (40 cases) and research group (40 cases), the control group was given the basic treatment, and the research group was given levosimendan on the basis of the control group, after 1 weeks’ treatment, to compare the clinical curative effect, LVESD, LVEDD, LVEF, hs-CRP, IL-6, TNF-α.Results:Comparing with the before treatment, the LVEDD, LVESD, hs-CRP, TNF-α, IL-6 in two groups after treatment decreased, and LVEF increased, the difference were statistically significant. Comparing with control group after treatment, the LVEDD, LVESD, hs-CRP, TNF-α, IL-6 in research group after treatment decreased obviously, and LVEF increased obviously, the difference were statistically significant.Conclusion:It has great clinical curative effect that levosimendan treat elderly patients with acute myocardial infarction complicated heart failure, it can ameliorate heart function and inflammation reaction, safe and reliable, and it is worthy of application.

  2. The H2S Donor NaHS Changes the Expression Pattern of H2S-Producing Enzymes after Myocardial Infarction.

    Science.gov (United States)

    Li, Na; Wang, Ming-Jie; Jin, Sheng; Bai, Ya-Dan; Hou, Cui-Lan; Ma, Fen-Fen; Li, Xing-Hui; Zhu, Yi-Chun

    2016-01-01

    Aims. To examine the expression patterns of hydrogen sulphide- (H2S-) producing enzymes in ischaemic heart tissue and plasma levels of H2S after 2 weeks of NaHS treatment after myocardial infarction (MI) and to clarify the role of endogenous H2S in the MI process. Results. After MI surgery, 2 weeks of treatment with the H2S donor NaHS alleviated ischaemic injury. Meanwhile, in ischemia myocardium, three H2S-producing enzymes, cystathionine γ-lyase (CSE), cystathionine-β-synthase (CBS), and 3-mercaptopyruvate sulfurtransferase (3-MST) significantly increased. Plasma H2S levels were also elevated. In vitro, NaHS treatment protected cardiomyocytes from hypoxic injury and raised CBS levels in a concentration-dependent manner. Different from in vivo results, however, CSE or 3-MST expression did not change. NaHS treatment increased the activity of CSE/CBS but not of 3-MST. When CSE was either knocked down (in vitro) or knocked out (in vivo), H2S levels significantly decreased, which subsequently exacerbated the ischaemic injury. Meanwhile, the expressions of CBS and 3-MST increased due to compensation. Conclusions. Exogenous H2S treatment changed the expressions of three H2S-producing enzymes and H2S levels after MI, suggesting a new and indirect regulatory mechanism for H2S production and its contribution to cardiac protection. Endogenous H2S plays an important role in protecting ischaemic tissue after MI.

  3. H2S Donor NaHS Changes the Production of Endogenous H2S and NO in D-Galactose-Induced Accelerated Ageing.

    Science.gov (United States)

    Wu, Wei; Hou, Cui-Lan; Mu, Xue-Pan; Sun, Chen; Zhu, Yi-Chun; Wang, Ming-Jie; Lv, Qian-Zhou

    2017-01-01

    Aims. The study was designed to explore whether hydrogen sulphide (H2S) and nitric oxide (NO) generation changed in D-galactose- (D-gal-) induced ageing, the possible effects of exogenous H2S supplementation, and related mechanisms. Results. In D-gal-induced senescent mice, both H2S and NO levels in the heart, liver, and kidney tissues were decreased significantly. A similar trend was observed in D-gal-challenged human umbilical vein endothelial cells (HUVECs). Sustained H2S donor (NaHS) treatment for 2 months elevated H2S and NO levels in these mice, and during this period, the D-gal-induced senescent phenotype was reversed. The protective effect of NaHS is associated with a decrease in reactive oxygen species levels and an increase in antioxidants, such as glutathione, and superoxide dismutase and glutathione peroxidase activities. Increased expression of the H2S-producing enzymes cystathionine γ-lyase (CSE) and cystathionine-β-synthase (CBS) in the heart, liver, and kidney tissues was observed in the NaHS-treated groups. NaHS supplementation also significantly postponed D-gal-induced HUVEC senescence. Conclusions. Endogenous hydrogen sulphide production in both ageing mice and endothelial cells is insufficient. Exogenous H2S can partially rescue ageing-related dysfunction by inducing endogenous H2S and NO production and reducing oxidative stress. Restoring endogenous H2S production may contribute to healthy ageing, and H2S may have antiageing effects.

  4. A large multi-centre European study validates high-sensitivity C-reactive protein (hsCRP) as a clinical biomarker for the diagnosis of diabetes subtypes

    DEFF Research Database (Denmark)

    Thanabalasingham, G.; Shah, N.; Vaxillaire, M.;

    2011-01-01

    . High-sensitivity CRP levels were analysed in individuals with HNF1A-MODY (n = 457), glucokinase (GCK)-MODY (n = 404), hepatocyte nuclear factor 4 alpha (HNF4A)-MODY (n = 54) and type 2 diabetes (n = 582) from seven European centres. Three common assays for hsCRP analysis were evaluated. We excluded 121...

  5. Proposal to conserve the name Phomopsis citri H.S. Fawc. (Diaporthe citri), with a conserved type, against Phomopsis citri (Sacc.) Traverso & Spessa (Ascomycota, Diaporthales, Diaporthaceae)

    Science.gov (United States)

    The name Diaporthe citri applies to a fungus that causes a disease on Citrus known as melanose or stem end rot of mature fruit after harvest and occurs widely in North America and Asia. Initially described as the illegitimate Phomopsis citri H.S. Fawc. 1912, non P. citri (Sacc.) Traverso & Spessa 19...

  6. Volatiles in Male Flowers of Cucurbita moschata by HS-SPME-GC-MS%HS-SPME-GC-MS分析金钩南瓜雄花挥发性成分

    Institute of Scientific and Technical Information of China (English)

    张伟; 卢引; 李昌勤; 康文艺

    2012-01-01

    目的:分析金钩南瓜雄花挥发性成分.方法:采用顶空固相微萃取和气质联用技术(HS-SPME-GC-MS)结合保留指数法,并用峰面积归一化法测定相对百分含量.结果:从金钩南瓜雄花中鉴定出59个化合物,占总峰面积的90.99%.结论:研究表明α-佛手柑油烯含量最高,占总挥发性成分的21.71%.该研究可为进一步开发利用南瓜资源提供科学依据.%Objective: To study the volatile constituents in male flower of Cucurbita moschata. Method; The volatiles were analyzed by head-space solid micro-extraction, coupled with GC-MS and Kovats indices. A quantitative analysis in percent was performed by peak area normalization measurements. Result; Fifty-nine compounds were identified in Jingou accounting for 90.99% of the total volatiles. Conclusion; a-bergamotene (21.71% ) was the highest. The study can provide scientific basis for the further development of the seeds of C. moschata.

  7. Evolution of human IgH3'EC duplicated structures: both enhancers HS1,2 are polymorphic with variation of transcription factor's consensus sites.

    Science.gov (United States)

    Giambra, Vincenzo; Fruscalzo, Alberto; Giufre', Maria; Martinez-Labarga, Cristina; Favaro, Marco; Rocchi, Mariano; Frezza, Domenico

    2005-02-14

    The enhancer complex regulatory region at the 3' of the immunoglobulin heavy cluster (IgH3'EC) is duplicated in apes along with four constant genes and the region is highly conserved throughout humans. Both human IgH3'ECs consist of three loci high sensitive (HS) to DNAse I with enhancer activity. It is thus possible that the presence of structural divergences between the two IgH3'ECs and of relative polymorphisms correspond to functional regulatory changes. To analyse the polymorphisms of these almost identical regions, it resulted mandatory to identify the presence of divergent sequences, in order to select distinctive primers for specific PCR genomic amplifications. To this aim, we first compared the two entire IgH3'ECs in silicio, utilising the updated GenBank (GB) contigs, then we analysed the two IgH3'ECs by cloning and sequencing amplicons from independent genomes. In silicio analysis showed that several inversions, deletions and short insertions had occurred after the duplication. We analysed in detail, by sequencing specific regions, the polymorphisms occurring in enhancer HS1,2-A (which lies in IgH3'EC-1, 3' to the Calpha-1 gene) and in enhancer HS1,2-B (which lies in IgH3'EC-2, 3' to Calpha-2). Polymorphisms are due to the repetition (occurring one to four times) of a 38-bp sequence present at the 3' of the core of enhancers HS1,2. The structure of both human HS1,2 enhancers has revealed not yet described polymorphic features due to the presence of variable spacer elements separating the 38-bp repetitions and to variable external elements bordering the repetition cluster. We found that one of the external elements gave rise to a divergent allele 3 in the two clusters. The frequency of the different alleles of the two loci varies in the Italian population and allele 3 of both loci are very rare. The analysis of the Callicebus moloch, Gorilla gorilla and Pan troglodytes HS1,2 enhancers showed the transformation from the ancestral structure with the 31- to

  8. Cytokines and hs-CRP levels in individuals treated with low-dose aspirin for cardiovascular prevention: a population-based study (CoLaus Study).

    Science.gov (United States)

    Vaucher, Julien; Marques-Vidal, Pedro; Waeber, Gérard; Vollenweider, Peter

    2014-04-01

    Pro-inflammatory cytokines and high-sensitive C-reactive protein (hs-CRP) are associated with increased risk for cardiovascular disease. Low-dose aspirin for CV prevention is reported to have anti-inflammatory effects. The aim of this study was to determine the association between pro-inflammatory cytokines and hs-CRP levels and low-dose aspirin use for cardiovascular prevention in a population-based cohort (CoLaus Study). We assessed blood samples in 6085 participants (3201 women) aged 35-75years. Medications' use and indications were recorded. Among aspirin users (n=1'034; 17%), overall low-dose users (351; 5.8%) and low-dose for cardiovascular prevention users (324; 5.3%) were selected for analysis. Pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α were assessed by a multiplex particle-based flow cytometric assay and hs-CRP by an immunometric assay. Cytokines and hs-CRP were presented in quartiles. Multivariate analysis adjusting for sex, age, smoking status, body mass index, diabetes mellitus and immunomodulatory drugs showed no association between cytokines and hs-CRP levels and low-dose aspirin use for cardiovascular prevention, either comparing the topmost vs. the three other quartiles (OR 95% CI, 0.84 (0.59-1.18), 1.03 (0.78-1.32), 1.10 (0.83-1.46), 1.00 (0.67-1.69) for IL-1β, IL-6, TNF-α and hs-CRP, respectively), or comparing the topmost quartile vs. the first one (OR 95% CI, 0.87 (0.60-1.26), 1.19 (0.79-1.79), 1.26 (0.86-1.84), 1.06 (0.67-1.69)). Low-dose aspirin use for cardiovascular prevention does not impact plasma pro-inflammatory cytokine and hs-CRP levels in a population-based cohort.

  9. Evaluation in an emergency department of rapid separator tubes containing thrombin for serum preparation prior to hs-cTnT and CK-MB analyses.

    Science.gov (United States)

    Budak, Yasemin U; Huysal, Kagan; Bulut, Mehtap; Polat, Murat

    2013-01-01

    In our emergency department, we collect blood in Rapid Serum Tubes (RSTs; Becton Dickinson, Franklin Lakes, NJ), in which clotting times are reduced. We investigated the influence of RST use on cardiac troponin T (hs-cTnT) and creatine kinase-MB (CK-MB) test results, in comparison with the use of tubes featuring a separator gel containing a clotting activator (SSTs; Green-vac, Yongin, Korea). Samples from 60 patients were divided into equal aliquots and placed into RSTs and SSTs; hs-cTnT and CK-MB concentrations were determined using an autoanalyzer (Elecsys 2010) running commercial assays (Roche Diagnostics, Penzberg, Germany). Between-tube differences in CK-MB and hs-cTnT values were compared using the paired t-test, and correlations among variables were evaluated by calculation of Spearman correlation coefficients (r values). Deming regression analysis was performed and Bland-Altman plots were constructed. The hs-cTnT and CK-MB test results obtained from samples placed into RSTs and SSTs did not differ (p > 0.1). The correlations between the concentrations of hs-cTnT and CK-MB in samples placed into RSTs and SSTs were good; both r values were unity (p MB] = 0.95 SST [CK-MB]-0.09 ng/ml. The biases of 1.4 pg/ml (95% CI: minus 8.1-10.7 pg/ml) for hs-cTnT levels and 0.249 ng/ml (95% CI: minus 0.682-1.681 ng/ml) for CK-MB levels assayed using either tube was acceptable. The hs-cTnT and CK-MB test results did not significantly differ when either tube was used. RST tube use was associated with a short clotting time; this was an advantage in an emergency laboratory setting.

  10. A network of HSPG core proteins and HS modifying enzymes regulates netrin-dependent guidance of D-type motor neurons in Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Stephan Gysi

    Full Text Available Heparan sulfate proteoglycans (HSPGs are proteins with long covalently attached sugar side chains of the heparan sulfate (HS type. Depending on the cellular context HS chains carry multiple structural modifications such as sulfate residues or epimerized sugars allowing them to bind to a wide range of molecules. HSPGs have been found to play extremely diverse roles in animal development and were shown to interact with certain axon guidance molecules. In this study we describe the role of the Caenorhabditis elegans HSPG core proteins Syndecan (SDN-1 and Glypican (LON-2 and the HS modifying enzymes in the dorsal guidance of D-type motor axons, a process controlled mainly by the conserved axon guidance molecule UNC-6/Netrin. Our genetic analysis established the specific HS code relevant for this axon guidance event. Using two sensitized genetic backgrounds, we isolated novel components influencing D-type motor axon guidance with a link to HSPGs, as well as new alleles of several previously characterized axon guidance genes. Interestingly, the dorsal axon guidance defects induced by mutations in zfp-1 or lin-35 depended on the transgene oxIs12 used to visualize the D-type motor neurons. oxIs12 is a large multi-copy transgene that enlarges the X chromosome by approximately 20%. In a search for genes with a comparable phenotype we found that a mutation in the known dosage compensation gene dpy-21 showed similar axon guidance defects as zfp-1 or lin-35 mutants. Thus, derepression of genes on X, where many genes relevant for HS dependent axon guidance are located, might also influence axon guidance of D-type motor neurons.

  11. A Network of HSPG Core Proteins and HS Modifying Enzymes Regulates Netrin-Dependent Guidance of D-Type Motor Neurons in Caenorhabditis elegans

    Science.gov (United States)

    Gysi, Stephan; Rhiner, Christa; Flibotte, Stephane; Moerman, Donald G.; Hengartner, Michael O.

    2013-01-01

    Heparan sulfate proteoglycans (HSPGs) are proteins with long covalently attached sugar side chains of the heparan sulfate (HS) type. Depending on the cellular context HS chains carry multiple structural modifications such as sulfate residues or epimerized sugars allowing them to bind to a wide range of molecules. HSPGs have been found to play extremely diverse roles in animal development and were shown to interact with certain axon guidance molecules. In this study we describe the role of the Caenorhabditis elegans HSPG core proteins Syndecan (SDN-1) and Glypican (LON-2) and the HS modifying enzymes in the dorsal guidance of D-type motor axons, a process controlled mainly by the conserved axon guidance molecule UNC-6/Netrin. Our genetic analysis established the specific HS code relevant for this axon guidance event. Using two sensitized genetic backgrounds, we isolated novel components influencing D-type motor axon guidance with a link to HSPGs, as well as new alleles of several previously characterized axon guidance genes. Interestingly, the dorsal axon guidance defects induced by mutations in zfp-1 or lin-35 depended on the transgene oxIs12 used to visualize the D-type motor neurons. oxIs12 is a large multi-copy transgene that enlarges the X chromosome by approximately 20%. In a search for genes with a comparable phenotype we found that a mutation in the known dosage compensation gene dpy-21 showed similar axon guidance defects as zfp-1 or lin-35 mutants. Thus, derepression of genes on X, where many genes relevant for HS dependent axon guidance are located, might also influence axon guidance of D-type motor neurons. PMID:24066155

  12. Association between prepulse inhibition of the startle response and latent inhibition of two-way avoidance acquisition: A study with heterogeneous NIH-HS rats.

    Science.gov (United States)

    Sánchez-González, Ana; Esnal, Aitor; Río-Álamos, Cristóbal; Oliveras, Ignasi; Cañete, Toni; Blázquez, Gloria; Tobeña, Adolf; Fernández-Teruel, Alberto

    2016-03-01

    This study presents the first evaluation of the associations between responses in two paradigms related to schizophrenia in the genetically heterogeneous NIH-HS rat stock. NIH-HS rats are a stock of genetically heterogeneous animals that have been derived from eight different inbred strains. A rotational breeding schedule has been followed for more than eighty generations, leading to a high level of genetic recombination that makes the NIH-HS rats a unique tool for studying the genetic basis of (biological, behavioral, disease-related) complex traits. Previous work has dealt with the characterization of coping styles, cognitive and anxiety/fear-related profiles of NIH-HS rats. In the present study we have completed their characterization in two behavioral models, prepulse inhibition (PPI) and latent inhibition (LI) of the two-way active avoidance response, that appear to be related to schizophrenia or to schizophrenia-relevant symptoms. We have found that these rats display PPI for each of the four prepulse intensities tested, allowing their stratification in high, medium and low PPI subgroups. When testing these three subgroups for LI of two-way active avoidance acquisition it has been observed that the LowPPI and MediumPPI subgroups present impaired LI, which, along with the fact that the HighPPI group presents significant LI, allows us to hypothesize that responses in these two paradigms are somehow related and that selection of NIH-HS rats for Low vs HighPPI could make a promising animal model for the study of clusters of schizophrenia-relevant symptoms and their underlying neurobiological mechanisms.

  13. COPD患者血清IL-6、IL-8、hs-CRP和IL-18检测的临床意义%Clinical Relevance of Determination the Changes on Serum IL-6, IL-8,hs-CRP and IL-18 Levels in Patients with Chronic Obstructive Pulmonary Diseases(COPD)

    Institute of Scientific and Technical Information of China (English)

    赵庆琪; 姚加平

    2012-01-01

    目的:观察慢性阻塞性肺疾病(COPD)患者治疗前后血清IL-6、IL-8、hs-CRP和IL-18水平的变化及临床意义.方法:应用放射免疫分析和免疫比浊法对32例COPD患者进行了治疗前后血清IL-6、IL-8、hs-CRP和IL-8检测,并与35名正常健康人作比较.结果:COPD患者在治疗前血清IL-6、IL-8、hs-CRP和IL-18水平均非常显著地高于正常人组(P<0.01),经中西医结合治疗1个月后与正常人组比较仍有差异(P<0.05).结论:IL-6、IL-8、hs-CRP和IL-18的测定,可适用为一种筛选方法,其变化可能以不同的方式参与了COPD的发病,此外,该些项目的检测对了解病情、指导治疗具有重要的临床价值.%Objective To observe the clinical significance of changes on serum IL-6, IL-8, hs-CRP and BL-18 levels after treatment in patients with COPD. Methods Serum 11-6, IL-8 (with RIA) , serum hs-CRP(with immunotubidimetry) levels were measured both before and after treatment in 32 patients with COPD as well as in 35 normal controls. Results Before treatment, the serum IL-6, IL-8, hs-CRP, IL-18 levels were significantly higher than those in controls (P < 0.01). After 1 month of treatment the serum IL-6, IL-8, hs-CRP and IL-18 dropped markedly but remained higher difference than those in controls ( P < 0.05 ). Conclusion Measurement of serum IL-6,IL-8,hs-CRP and IL-18 were suitable to be used as a screening method. Besides, it could take part in pathogenesis of COPD in various ways and the changes of these items levels is of important values to realize pathosis and therapeutic effect.

  14. Clinical Significance of Measurement of Serum hs-CRP,IL-8 and IL-10 Levels in Patients with Periodontitis%牙周病患者血清hs-CRP、IL-8和IL-10检测的临床意义

    Institute of Scientific and Technical Information of China (English)

    王惠

    2012-01-01

    Objective To explore the significance of changes on serum hs-CRP,IL-8 and IL-10 levels in patients with periodonti-tis. MethOtS Serum hs-CRP(with immuno-turbidimetry) ,IL-8 (with RIA) ,IL-10 (with ELISA) levels were measured in 32 patients with periodontitis both before and after treatment as well as in 35 normal controls. Results Before treatment serum levels of hs-CRP, IL-8 and IL-10 were extremely higher in the patients than those in controls (P<0.01). After one month of treatment, the serum hs-CRP,IL-8 and IL-10 levels decreased but still significantly higher than those in controls (P <0.05 ) Conclusion Determination of Changes of serum hs-CRP,IL-8 and IL-10 levels has definite value to study pathogenesis, prevention and guide the therapeutic effect in patients with periodontitis.%目的:探讨牙周病患者血清hs-CRP、IL-8和IL-10水平的变化及临床意义.方法:应用放射免疫分析、酶联法和免疫比浊法对32例牙周病患者进行了血清hs-CRP、IL-8和IL-10检测,并与35名正常人作比较.结果:牙周病患者在治疗前血清hs-CRP、IL-8和IL-10水平均非常显著地高于正常人组(P<0.01),经治疗1个月后与正常人组比较仍有显著性差异(P<0.05).结论:检测血清hs-CRP、IL-8和IL-10水平的变化,对探讨牙周病发病机制、预防和指导治疗均有一定的临床价值.

  15. Tribocorrosion mechanisms of Ti6Al4V biomedical alloys in artificial saliva with different pHs

    Science.gov (United States)

    Licausi, M. P.; Igual Muñoz, A.; Amigó Borrás, V.

    2013-10-01

    Titanium and its alloys has been widely used for the design of dental implants because of its biocompatibility, mechanical properties and corrosion resistance. The powder-metallurgy process is a promising alternative to the casting fabrication process of titanium alloys for bone implants design as the porous structure mimics the natural bone structures, allowing the bone to grow into the pores which results in a better fixation of the artificial implant. However, under in vivo conditions the implants are subjected to tribocorrosion phenomenon, which consists in the degradation mechanisms due to the combined effect of wear and corrosion. The aim of this study is to evaluate the tribocorrosion behaviour of cast and sintered Ti6Al4V biomedical alloy for dental applications using the cast material as reference. Titanium samples were tested in artificial human saliva solution with three different pHs (3, 6, 9) and in an acidic saliva with 1000 ppm fluorides (AS-3-1000F-) by different electrochemical techniques (potentiodynamic curves, potentiostatic tests and tribo-electrochemical tests). Cast and sintered titanium alloys exhibit the same tribocorrosion mechanisms in AS independently of the pH which consists in plastic deformation with passive dissolution, but the addition of fluorides to the acidified solution changes the degradation mechanism towards active dissolution of the titanium alloys.

  16. Permeation of tecnazene through human skin in vitro as assessed by HS-SPME and GC-MS.

    Science.gov (United States)

    Bhatt, Varsha D; Soman, Rajiv S; Miller, Matthew A; Kasting, Gerald B

    2008-09-01

    Permeation of tecnazene into and through human cadaver skin in vitro was assessed using a CC-MS method employing HS-SPME for receptor solution analyses. Two doses of tecnazene dissolved in acetone, corresponding to 103 and 864 microg/cm2 of tecnazene, were applied to skin mounted on Franz diffusion cells and placed in a fume hood. Cells were either occluded with aluminum foil or left unoccluded. Total absorption of tecnazene (dermis + receptor fluid) after 48 h was 2.2-6.1% of the applied dose for the unoccluded treatments and 22-33% for the occluded treatments. Potentially absorbed dose including all tecnazene that may have eventually permeated the skin ranged from 10% unoccluded to 42-53% occluded. Accumulation in the receptor solutions was satisfactorily described by a working diffusion model after upward adjustment of the partition coefficient for tecnazene in all skin layers by a factor of 5-16 versus a priori values. However, residual amounts of tecnazene in both the epidermis and dermis were higher than those estimated from the model, suggesting the existence of tissue binding not accounted for in the calculation. The results indicate that the diffusion model as presently calibrated may significantly underestimate both systemic absorption and skin concentrations of highly lipophilic compounds, as predicted from data generated from in vitro skin permeation assays. Model predictions could be improved by better accounting for partitioning into the epidermis and dermis.

  17. Inactivation of Escherichia coli by photochemical reaction of ferrioxalate at slightly acidic and near-neutral pHs.

    Science.gov (United States)

    Cho, Min; Lee, Yunho; Chung, Hyenmi; Yoon, Jeyong

    2004-02-01

    Fenton chemistry, which is known to play an effective role in degrading toxic chemicals, is difficult to apply to disinfection in water treatment, since its reaction is effective only at the acidic pH of 3. The presence of oxalate ions and UV-visible light, which is known as a photoferrioxalate system, allows the Fe(III) to be dissolved at slightly acidic and near-neutral pHs and maintains the catalytic reaction of iron. This study indicates that the main oxidizing species in the photoferrioxalate system responsible for microorganism inactivation is OH radical. Escherichia coli was used as an indicator microorganism. The CT value (OH radical concentration x contact time; used to indicate the effect of the combination of the concentration of the disinfectant and the contact time on inactivation) for a 2-log inactivation of E. coli was approximately 1.5 x 10(-5) mg/liter/min, which is approximately 2,700 times lower than that of ozone as estimated by the delayed Chick-Watson model. Since the light emitted by the black light blue lamp is similar to sunlight in the specific wavelength range of 300 to 420 nm, the photoferrioxalate system, which can have a dual function, treating water for both organic pollutants and microorganisms simultaneously, shows promise for the treatment of water or wastewater in remote or rural sites. However, the photoferrioxalate disinfection system is slower in inactivating microorganisms than conventional disinfectants are.

  18. Inoculum type response to different pHs on biohydrogen production from L-arabinose, a component of hemicellulosic biopolymers

    Energy Technology Data Exchange (ETDEWEB)

    Abreu, A.A.; Danko, A.S.; Costa, J.C.; Ferreira, E.C.; Alves, M.M. [IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga (Portugal)

    2009-02-15

    Biohydrogen production from arabinose was examined using four different anaerobic sludges with different pHs ranging from 4.5 to 8.0. Arabinose (30 g l{sup -1}) was used as the substrate for all experiments. Individual cumulative hydrogen production data was used to estimate the three parameters of the modified Gompertz equation. Higher hydrogen production potentials were observed for higher pH values for all the sludges. G2 (acclimated granular sludge) showed the highest hydrogen production potential and percentage of arabinose consumption compared to the other sludges tested. Granular sludges (G1 and G2) showed different behaviour than the suspended sludges (S1 and S2). The differences were observed to be smaller lag phases, the percentage of acetate produced, the higher percentage of ethanol produced, and the amount of arabinose consumed. A high correlation (R{sup 2} = 0.973) was observed between the percentage of n-butyrate and the percentage of ethanol in G1 sludge, suggesting that ethanol/butyrate fermentation was the dominant fermentative pathway followed by this sludge. In S1, however, the percentage of n-butyrate was highly correlated with the percentage of acetate (R{sup 2} = 0.980). This study indicates that granular sludge can be used for larger pH ranges without reducing its capacity to consume arabinose and achieve higher hydrogen production potentials. (author)

  19. Putting Citizen-Collected Observations to Work -- The Community Collaborative Rain, Hail and Snow Network (CoCoRaHS)

    Science.gov (United States)

    Doesken, N.

    2015-12-01

    When CoCoRaHS was born (1998), climate-relevant information was far from our minds. We were simply enlisting volunteers to help capture, display and communicate the nature of small scale variability within northern Colorado storms. Climate change was talked about then, but not with the sense of concern and urgency as today. Now, many years later, the simple back-yard precipitation measurements being taken by thousands of volunteers across much of North America are creating valuable and easily-accessible data and information serving many and varied purposes from federal and state climate monitoring to drought and extreme storm analysis and research. Many volunteers have been with the project now for a decade or longer and have contributed literally thousands of individual observations and reports. Long-time participants along with recent recruits of all ages are seeing first-hand how day by day observations of weather conditions combine - over time and space -- to define and describe key elements of our climate and its variations. The fact that the data from volunteers are frequently used and applied by scientists and decision makers is one of the key factors in retaining long-term volunteers. Examples will be presented of volunteer precipitation data being used both independently and in combination with data from federal monitoring systems. Challenges of maintaining a large volunteer network will be discussed along with some plans and opportunities for the future.

  20. The temporal spectrum of the sdB pulsating star HS 2201+2610 at 2 ms resolution

    Science.gov (United States)

    Silvotti, R.; Janulis, R.; Schuh, S. L.; Charpinet, S.; Oswalt, T.; Silvestri, N.; Gonzalez Perez, J. M.; Kalytis, R.; Meištas, E.; Ališauskas, D.; Marinoni, S.; Jiang, X. J.; Reed, M. D.; Riddle, R. L.; Bernabei, S.; Heber, U.; Bärnbantner, O.; Cordes, O.; Dreizler, S.; Goehler, E.; Østensen, R.; Bochanski, J.; Carlson, G.

    2002-07-01

    In this article we present the results of more than 180 hours of time-series photometry on the low gravity (log g=5.4, Teff=29 300 K, log He/H=-3.0 by number) sdB pulsating star HS 2201+2610, obtained between September 2000 and August 2001. The temporal spectrum is resolved and shows 5 close frequencies: three main signals at 2860.94, 2824.10 and 2880.69 mu Hz, with amplitudes of about 1%, 0.5% and 0.1% respectively, are detected from single run observations; two further peaks with very low amplitude (Max-Plank-Institute für Astronomie Heidelberg jointly with the Spanish National Commission for Astronomy), SARA 0.9 m (Southeastern Association for Research in Astronomy, at Kitt Peak, Arizona), Tenerife 0.8 m (Instituto de Astrofisica de Canarias), NOT 2.6 m (operated on the island of La Palma jointly by Denmark, Finland, Iceland, Norway, and Sweden, in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofisica de Canarias), Beijing 0.85 m (Beijing Astronomical Observatory), Fick 0.6 m (Iowa State University), Wendelstein 0.8 m (University of Munich).

  1. Computer simulation of the CSPAD, ePix10k, and RayonixMX170HS X-ray detectors

    Energy Technology Data Exchange (ETDEWEB)

    Tina, Adrienne

    2015-08-21

    The invention of free-electron lasers (FELs) has opened a door to an entirely new level of scientific research. The Linac Coherent Light Source (LCLS) at SLAC National Accelerator Laboratory is an X-ray FEL that houses several instruments, each with its own unique X-ray applications. This light source is revolutionary in that while its properties allow for a whole new range of scientific opportunities, it also poses numerous challenges. For example, the intensity of a focused X-ray beam is enough to damage a sample in one mere pulse; however, the pulse speed and extreme brightness of the source together are enough to obtain enough information about that sample, so that no further measurements are necessary. An important device in the radiation detection process, particularly for X-ray imaging, is the detector. The power of the LCLS X-rays has instigated a need for better performing detectors. The research conducted for this project consisted of the study of X-ray detectors to imitate their behaviors in a computer program. The analysis of the Rayonix MX170-HS, CSPAD, and ePix10k in particular helped to understand their properties. This program simulated the interaction of X-ray photons with these detectors to discern the patterns of their responses. A scientist’s selection process of a detector for a specific experiment is simplified from the characterization of the detectors in the program.

  2. The Wide-Angle Outflow of the Lensed z = 1.51 AGN HS 0810+2554

    CERN Document Server

    Chartas, G; Hamann, F; Eracleous, M; Strickland, S; Giustini, M; Misawa, T

    2016-01-01

    We present results from X-ray observations of the gravitationally lensed z = 1.51 AGN HS 0810+2554 performed with the Chandra X-ray Observatory and XMM-Newton. Blueshifted absorption lines are detected in both observations at rest-frame energies ranging between ~1-12 keV at > 99% confidence. The inferred velocities of the outflowing components range between ~0.1c and ~0.4c. A strong emission line at ~6.8 keV accompanied by a significant absorption line at ~7.8 keV is also detected in the Chandra observation. The presence of these lines is a characteristic feature of a P-Cygni profile supporting the presence of an expanding outflowing highly ionized iron absorber in this quasar. Modeling of the P-Cygni profile constrains the covering factor of the wind to be > 0.6, assuming disk shielding. A disk-reflection component is detected in the XMM-Newton observation accompanied by blueshifted absorption lines. The XMM-Newton observation constrains the inclination angle to be < 45 degrees at 90% confidence, assuming...

  3. An environmental scan of an aged care workplace using the PARiHS model: assessing preparedness for change.

    Science.gov (United States)

    Gibb, Heather

    2013-03-01

    The environmental scan aimed to deepen our understanding of the aged care work culture and to ascertain the readiness of the workers to advance towards team-based quality care provision. The workplace context was a high-care unit within a large residential aged care facility. We used the Promoting Action on Research Implementation in Health Services (PARiHS) framework to assess workplace readiness via interviews, individual surveys and observation of practice. A profile of current culture emerged as mutually supportive and task focused, but at the same time lacking corporate team features of shared decision-making and feedback for practice improvement. However, latent within the frontline leaders and personal care staff, there was evidence of some embedded knowledge and capacity for corporate team performance. This study has validated an evidence-based method for conducting environmental scanning in aged care, recommended before any major change is introduced. Environmental scanning helps gauge workforce capacity and limitations; this information can enable managers to capitalize on identified cultural strengths to fortify change and avoid pitfalls of personal and collective vulnerabilities. © 2012 Blackwell Publishing Ltd.

  4. Identification of volatile components in Chinese Sinkiang fermented camel milk using SAFE, SDE, and HS-SPME-GC/MS.

    Science.gov (United States)

    Ning, Li; Fu-Ping, Zheng; Hai-Tao, Chen; Si-Yuan, Liu; Chen, Gu; Zhen-Yang, Song; Bao-Guo, Sun

    2011-12-01

    The volatile components of Chinese Sinkiang fermented camel milk were isolated by solvent assisted flavour evaporation (SAFE), simultaneous distillation extraction (SDE, dichloromethane and diethyl ether as solvent, respectively) and headspace solid-phase microextraction (HS-SPME, CAR/PDMS, PDMS/DVB and DVB/CAR/PDMS fibre extraction, respectively) and analysed by GC/MS. A total of 133 volatile components were identified under 6 different conditions, including 30 esters, 20 acids, 18 saturated alcohols, 15 unsaturated aliphatic alcohols, 8 saturated ketones, 9 saturated aldehydes, 8 unsaturated aliphatic aldehydes, 6 furans, 5 sulphur-containing compounds, 5 ethers, 5 lactones, 3 other compounds, and 1 unsaturated aliphatic ketone. Three pretreatment methods were compared, assisted by principal component analysis (PCA). The results indicated that the volatile components obtained using different methods varied greatly both in categories and in content, and therefore, a multi-pretreatment method should be adopted together with GC/MS. A total of 71 aroma-active compounds were detected by gas chromatography-olfactometry (GC-O), among which 66 aroma-active compounds were found by SDE (60, dichloromethane as solvent; 24, diethyl ether as solvent), 26 by SAFE.

  5. SIRT1 deacetylates SATB1 to facilitate MAR HS2-MAR ε interaction and promote ε-globin expression.

    Science.gov (United States)

    Xue, Zheng; Lv, Xiang; Song, Wei; Wang, Xing; Zhao, Guang-Nian; Wang, Wen-Tian; Xiong, Jian; Mao, Bei-Bei; Yu, Wei; Yang, Ben; Wu, Jie; Zhou, Li-Quan; Hao, De-Long; Dong, Wen-Ji; Liu, De-Pei; Liang, Chih-Chuan

    2012-06-01

    The higher order chromatin structure has recently been revealed as a critical new layer of gene transcriptional control. Changes in higher order chromatin structures were shown to correlate with the availability of transcriptional factors and/or MAR (matrix attachment region) binding proteins, which tether genomic DNA to the nuclear matrix. How posttranslational modification to these protein organizers may affect higher order chromatin structure still pending experimental investigation. The type III histone deacetylase silent mating type information regulator 2, S. cerevisiae, homolog 1 (SIRT1) participates in many physiological processes through targeting both histone and transcriptional factors. We show that MAR binding protein SATB1, which mediates chromatin looping in cytokine, MHC-I and β-globin gene loci, as a new type of SIRT1 substrate. SIRT1 expression increased accompanying erythroid differentiation and the strengthening of β-globin cluster higher order chromatin structure, while knockdown of SIRT1 in erythroid k562 cells weakened the long-range interaction between two SATB1 binding sites in the β-globin locus, MAR(HS2) and MAR(ε). We also show that SIRT1 activity significantly affects ε-globin gene expression in a SATB1-dependent manner and that knockdown of SIRT1 largely blocks ε-globin gene activation during erythroid differentiation. Our work proposes that SIRT1 orchestrates changes in higher order chromatin structure during erythropoiesis, and reveals the dynamic higher order chromatin structure regulation at posttranslational modification level.

  6. Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS

    Directory of Open Access Journals (Sweden)

    Bruna Claudia Coppe

    2016-01-01

    Full Text Available The use of oral fluid as a biological matrix to monitor the use of drugs of abuse is a global trend because it presents several advantages and good correlation to the blood level. Thus, the present work aimed to develop and validate an analytical method for quantification and detection of solvents used as inhalants of abuse in oral fluid (OF, using Quantisal™ as collector device by headspace and gas chromatography coupled with a mass detector (HS-GC/MS. Chromatographic separation was performed with a ZB-BAC1 column and the total time of analysis was 11.8 min. The method showed good linearity (correlation coefficient higher than 0.99 for all solvents. The limits of detection ranged from 0.05 to 5 mg/L, while the lower limits of quantification ranged from 2.5 to 12.5 mg/L. Accuracy, precision, matrix effect, and residual effect presented satisfactory results, meeting the criteria accepted for the validation of bioanalytical methods. The method showed good selectivity considering that, for solvents coeluting at the same retention time, resolution was performed by the mass detector. The method developed proved to be adequate when applied in OF samples from users of drugs and may be used to monitor the abuse of inhalants in routine forensic analyses.

  7. Decreased expression of alpha-2-HS glycoprotein in the sera of rats treated with Eurycoma longifolia extract

    Directory of Open Access Journals (Sweden)

    Yeng eChen

    2015-09-01

    Full Text Available Eurycoma longifolia is a Malaysian native herb that has been widely used as an aphrodisiac and a remedy for andropause. Although the physiological effects of the plant extract were predicted as a result of the alterations in protein expression, the key protein(s involved in these alterations are still unclear. In the present study, we have investigated the effect of standardized Eurycoma longifolia extract on serum protein expression up to 28 days following oral administration in rats. Serum protein profiles were analyzed by 2-dimensional electrophoresis, and altered proteins were identified via mass spectrometry. We observed that alpha-2-HS glycoprotein (AHS was significantly decreased in the serum of experimentally treated rats compared to controls. Moreover, reduction in AHS was confirmed using competitive enzyme-linked immunosorbent assay. AHS expression is known to be associated with insulin resistance and diabetes. Our data indicated that serum AHS was reduced in rats treated with standardized E. longifolia extract, and therefore form a prelude for further investigation into the effects of this natural extract in animal models involving infertility and diabetes.

  8. 支气管肺炎患儿治疗前后血清hs-CRP、IL-18和IL-1β检测的临床意义%Clinical significance of changes of serum hs-CRP,IL-18 and IL-1β levels in patients with bronchopneumonia

    Institute of Scientific and Technical Information of China (English)

    黄德芳; 刘党英

    2011-01-01

    目的 探讨支气管肺炎患儿治疗前后血清hs-CRP、IL-18和IL-1β含量变化及意义.方法 应用免疫比浊法和ELIA法对33例支气管肺炎患儿进行了治疗前后血清hs-CRP、IL-18和IL-1β的检测,并与35名正常健康儿作比较.结果 支气管肺炎患儿治疗前后血清hs-CRP、IL-18和IL-1β水平非常显著地高于正常人组(P<0.01)且血清hs-CRP、IL-18和IL-1β水平呈明显正相关(r=0.5084,0.6123,P<0.01).结论 血清hs-CRP、IL-18和IL-1β水平的变化与支气管肺炎的发生和发展有关.

  9. COPD患者治疗前后血清hs-CRP、TNF-α、IL-6、IL-8检测的临床意义%Clinical Significance of Determination of Serum hs-CRP, TNF-α, IL-6 and IL-8 Levels After Treatment in Patients with Chronic Obstructive Pulmonary Disease

    Institute of Scientific and Technical Information of China (English)

    胡蓉

    2007-01-01

    目的:探讨慢性阻塞性肺疾病(COPD)患者治疗前后血清hs-CRP、TNF-α、IL-6、IL-8水平的变化及意义.方法:应用放免法和免疫比浊法对46例COPD患者进行治疗前后血清hs-CRP、TNF-α、IL-6、IL-8检测,并与35名正常健康人作比较.结果:COPD患者在治疗前血清hs-CRP、TNF-α、IL-6、IL-8水平均非常显著地高于正常人组(P<0.01),综合治疗后2周,除hs-CRP水平与正常人比较无差异外,TNF-α、IL-6、IL-8水平与正常人组比较仍有显著性差异(P<0.05).结论:hs-CRP、TNF-α、IL-6、IL-8可能以不同的方式参与了COPD的发病,其水平的检测对于了解病情、指导治疗具有重要的临床价值.

  10. Changes and Significance of Serum hs-CRP Level in Patients with Gouty Arthritis or Rheumatoid Arthritis%痛风性关节炎及类风湿性关节炎患者血清hs-CRP水平的变化和意义

    Institute of Scientific and Technical Information of China (English)

    孙际明

    2010-01-01

    目的 观察痛风性关节炎(gouty arthritis)及类风湿性关节炎(rheumatoid arthritis,RA)患者血清中超敏C反应蛋白(hypersensitive C reactive protein,hs-CRP)水平的变化.方法 选择健康体检者42例为对照组,痛风性关节炎组30例,类风湿性关节炎组20例及高尿酸血症组40例用全自动生化分析仪分别检测血清中hs-CRP、UA、RF的水平并进行统计学分析.结果 痛风性关节炎组及类风湿性关节炎组hs-CRP水平明显高于对照组(P<0.05),痛风性关节炎组hs-CRP水平明显高于高尿酸血症组(P<0.05).结论 hs-CRP可作为痛风性关节炎和类风湿性关节炎的诊断指标之一.

  11. HS-SPME结合GC-MS分析冷冻对欧李果香气的影响%Analysis of the Effects of Freezing on the Aroma of Cerasus Humilis by HS-SPME-GC-MS

    Institute of Scientific and Technical Information of China (English)

    周立华; 牟德华; 李艳

    2016-01-01

    香气影响水果的品质和加工特性。欧李果香气独特,但因成熟期短而集中给贮藏和加工带来困难,冷冻保藏是常用的方法。本研究采用顶空固相微萃取(HS-SPME)结合气质联用(GC-MS)技术检测了冷冻前后欧李果汁的香气成分,分析研究冷冻对欧李果香气的影响。结果表明,欧李果经-20℃冷冻保藏后,香气物质占总挥发性物质的含量减少了6.67%。冷冻前后共检出95种香气成分,其中,冷冻前检测出64种,冷冻后检测出65种。香气物质种类总数变化不大,主体香气物质为乙酸-3-甲基-3-丁烯-1-醇酯和梨醇酯,含量分别占冷冻前后总香气物质含量的44.87%和46.37%,但是这两种香气物质的比例发生了很大变化,乙酸-3-甲基-3-丁烯-1-醇酯冷冻后含量增加,从30.55%升高到40.52%。而梨醇酯经过冷冻从29.48%下降到16.93%。因此,冷冻不仅不会影响欧李果的加工特性,还增强了果实的成熟香气。%Aroma influences the quality and the processing of fruits. The aroma of Cerasus Humilis is unique, but its short maturity brings diffi-culty to the storage and fruit processing, and freezing storage is the commonly used method to solve the problem. In this study, the flavoring components of Cerasus Humilis juice before and after the freezing were detected by HS-SPME coupled with GC-MS, and the effects of freez-ing on the aroma of Cerasus Humilis were analyzed. The results suggested that, after freezing storage of Cerasus Humilis at-20℃, the content of flavoring components in total volatile substances decreased by 6.67%. 64 kinds of flavoring compounds were detected before the freezing and 65 kinds of flavoring compounds were detected after the freezing (95 kinds of flavoring compounds detected in total), and there was only a little change in flavoring compounds before and after the freezing. Butene-1-methyl-3-acetic acid esters and sorbitol esters were the

  12. Analysis of Volatile Constituents of Black Pepper (Piper nigrum L.)Leaves by HS-SPME-GC/MS%胡椒叶片挥发性成分HS-SPME-GC/MS分析

    Institute of Scientific and Technical Information of China (English)

    范睿; 郝朝运; 秦晓威; 徐飞; 邬华松

    2015-01-01

    以胡椒属种质蒌叶、墨西哥胡椒、假荜拔为材料,利用顶空固相微萃取-气相色谱和质谱联用(HS-SPME-GC/MS)技术测定3种种质叶片中的挥发性成分及用面积归一法计算其相对含量.结果表明:鉴定出蒌叶、墨西哥胡椒、假荜拔的挥发性成分59种,其中从蒌叶中共鉴定出挥发性成分36种,其含量较高的有安息香醛、荜澄茄油烯和β-石竹烯,相对含量分别为23.56%、9.36%和8.54%;从墨西哥胡椒中鉴定出挥发性成分18种,其中含量较高的有肉桂醛、萜品烯和萜品油烯,相对含量分别为77.98%、5.88%和5.11%;而假荜菝叶片挥发性成分有25种,含量较高的有β-石竹烯、罗勒烯和芳樟醇.挥发性成分的种类在3种胡椒属种质中都不相同,总的相对百分含量也不同,说明不同的种质,叶片挥发性成分有一定的差异.

  13. WEEE与RoHs

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    WEEE(Waste Electrical and Electronic Equipment)——废电机电子设备指令WEEE指令实施的目的,最主要就是防治电子电气废弃物,此外是实现这些废弃物的再利用,再循环使用和其它形式的回收,以减少废弃物的处理。同时也努力改进涉及电子电气设备生命周期的所有操作人员,如生产者、销售商、消费者,特别是直接涉及报废电子电器设备处理人员的环保行为。

  14. 牙周治疗对冠心病伴牙周炎患者血清HsCRP、TNF-α、IL-1β、IL-6水平的影响%The Effect of Periodontal Therapy on Serum HsCRP,TNF-α,IL-1β,IL-6 Level of Coronary Heart Disease Patients with Periodontitis

    Institute of Scientific and Technical Information of China (English)

    邓泽亚; 卢俊慧; 黎秀珍

    2014-01-01

    To investigate the effect of periodontal therapy on serum HsCRP,TNF-α,IL-1β,IL-6 level of coronary heart disease patients with periodontitis.Method:95 coronary heart disease patients with periodontitis were selected from June 2012 to June 2013 in our hospital during the period,all patients were given periodontal intervention.The serum TNF-α,HsCRP,IL-6,IL-1βlevel changes and(periodontitis)attachment level improved correlation were compared before the intervention and 6 months after intervention.Result:After treatment, the serum TNF-α,HsCRP,IL-6,IL-1β levels were decreased significantly,the differences were statistically significant(P<0.01),the serum TNF-α,HsCRP,IL-6,IL-1βlevels were negatively correlated with(periodontitis) attachment level,the differences were statistically significant(P<0.05).Conclusion:The periodontal treatment can effectively reduce serum HsCRP,TNF-α,IL-1β,IL-6 level of coronary heart disease patients with periodontitis, and serum TNF-α,HsCRP,IL-6,IL-1βlevels are negatively correlated with(periodontitis)attachment level,and to some extent can predict the degree of improvement in patients with periodontitis.%目的:探讨牙周治疗对冠心病伴牙周炎患者血清HsCRP、TNF-α、IL-1β、IL-6水平的影响。方法:选取2012年6月-2013年6月期间本院收治的95例冠心病伴牙周炎患者,均进行牙周综合干预,比较干预前与干预6个月后血清TNF-α,HsCRP、IL-6、IL-1β水平的变化及(牙周炎)附着水平改善的相关性。结果:治疗后患者血清TNF-α,HsCRP、IL-6、IL-1β水平均较治疗前显著降低,差异均有统计学意义(P<0.01);患者血清TNF-α,HsCRP、IL-6、IL-1β水平与(牙周炎)附着水平呈负相关,差异均有统计学意义(P<0.05)。结论:牙周治疗可有效降低冠心病伴牙周炎患者血清HsCRP、TNF-α、IL-1β、IL-6水平,且患者血清TNF-α、HsCRP、IL-6、IL-1β水平与(牙周炎)附着水平呈负

  15. CHANGES OF COPEPTIN, cTnI AND Hs-CRP AND THEIR CLINICAL SIGNIFICANCE IN PATIENTS WITH ACUTE CORONARY SYNDROME%急性冠状动脉综合征病人血浆和肽素、cTnI和 Hs-CRP变化及意义

    Institute of Scientific and Technical Information of China (English)

    闫小菊; 刘松; 李霞

    2011-01-01

    目的 探讨急性冠状动脉综合征(ACS)病人血浆和肽素、肌钙蛋白I(cTnI)与高敏C-反应蛋白(HsCRP)的变化及其临床意义.方法 因胸痛入院的病人196例,经冠状动脉造影术(CAG)检查后分为CAG正常组、不稳定型心绞痛(UAP)组、非ST段抬高急性心肌梗死(NSTEMI)组和ST段抬高急性心肌梗死(STEMI)组,测定各组病人入院时血浆和肽素、cTnI及Hs-CRP含量.结果 UAP组、NSTEMI组和STEMI组和肽素、cTnI及Hs-CRP水平高于CAG正常组(H=42.57~141.35,P<0.05);NSTEMI组和STEMI组和肽素、cTnI及HsCRP水平高于UAP组(H=9.72~107.45,P<0.05).左主干病变组较单支病变组、双支病变组、三支病变组和肽素、cTnI及Hs-CRP水平明显升高(H=7.26~35.72,P<0.05).结论 ACS病人血浆和肽素、cTnI及HsCRP水平升高,并且和肽素和cTnI在一定程度内可以反映冠状动脉病变严重程度,对ACS病人的危险分层、治疗决策及预后判断具有一定临床价值.%Objective To explore the clinical significance of C-terminal portion of provasopressin (Copeptin), cTnI and Hs-CRP in patients with acute coronary syndrome (ACS).Methods This study consisted of 194 patients who were hospitalized with chest pain.According to coronary angiography (CAG), they were classified into four groups as: CAG normal, unstable angina pectoris (UAP), non-ST elevation myocardial infarction (NSTEMI) and ST elevation myocardial infarction (STEM1).Plasma copeptin, cTnI and Hs-CRP were measured on admission.Results Plasma levels of copeptin, cTnI and Hs-CRP in patients of groups UAP, NSTEMI and STEMI were significantly higher than that in CAG normal group (H=42.57-141.35, P<0.05).The levels of copeptin, cTnI and Hs-CRP in patients of groups NSTEMI and STEMI were higher than that in UAP group (H=9.72-107.45,P<0.05).The patients with left main coronary stenosis had a higher level of copeptin, cTnI and Hs-CRP than those with a single-, double-or triple-branch coronary

  16. Effects and implications of physical modalities on hs-CRP, TNF-α and adiponectin in patients with cerebral infarction%物理因子治疗对脑梗死患者血清hs-CRP、TNF-α和脂联素的影响及其意义

    Institute of Scientific and Technical Information of China (English)

    阳小云; 王小梅; 陈昕; 侯敢; 杨明

    2008-01-01

    Objective To observe the effects of physical agents therapy on serum hs-CRP, TNF-α andadiponectin in patients with cerebral infarction and the possible underlying mechanisms. Methods Sixty patientswith cerebral infarction were randomly and equally divided into two groups: 30 cases were treated with physical a-gents therapy ( physical therapy group) , and 30 with drugs only ( drug treated group). Thirty normal subjectsserved as the control group. The level of hs-CRP in the serum was determined by latex agglutination reaction, TNF-and adiponectin were determined by using ELISA before and after therapy. Results The levels of serum hs-CRP and TNF-α of patients with cerebral infarction before therapy were much higher than those of the control group,but adiponectin was significantly lower than those of the control group( P < 0.01 ). After therapy, the levels of ser-um hs-CRP and TNF-α were decreased and adiponectin was increased significantly in both treated groups ( P <0.01 ). Comparison with two treated groups showed that the levels of hs-CRP and TNF-α were lower and adiponec-tin was obviously higher in physical agents therapy group than those in the drug treated group ( P < 0.05 ). Con-clusion The patients with cerebral infarction have low level of serum adiponectin. Physical therapy might exertbeneficial effects on patients with cerebral infarction by the decreasing serum hs-CRP and TNF-α, as well as by ele-vating adiponectin.%目的 观察物理因子治疗对脑梗死患者血清高敏C反应蛋白(hs-CRP)、肿瘤坏死因子α(TNF-α)和脂联素水平的影响,并探讨其可能机制.方法 60例脑梗死患者随机分为物理因子治疗组(综合组)30例和单纯药物治疗组(药物组)30例,并于治疗前、后用乳胶凝集反应法测定血清hs-CRP水平、ELISA法测定血清TNF-α和脂联素含量.并选取30例健康体检者作为正常对照组(对照组).结果 脑梗死患者治疗前,血清hs-CRP和TNF-α.

  17. HIGH-SENSITIVITY C-REACTIVE PROTEIN (hsCRP IN YOUNG ADULTS: RELATION TO AEROBIC CAPACITY, PHYSICAL ACTIVITY AND RISK FACTORS FOR CARDIOVASCULAR DISEASES

    Directory of Open Access Journals (Sweden)

    K. Mazurek

    2011-11-01

    Full Text Available Atheromatosis develops as a result of a chronic inflammatory process of the arteries. Inflammatory biomarkers, particularly high-sensitivity C-reactive protein (hsCRP, positively correlate with atheromatosis risk factors and can be used to estimate and predict the risk of cardiovascular events. The purpose of this study was to evaluate the relationship between hsCRP concentration and BMI, body composition, classical risk factors for cardiovascular diseases, energy expenditure for physical activity (WEE and  ·VO2max. 166 volunteers (78 women and 88 men were included in the examinations. Their mean age was 20.2±0.9 years. Health condition was described by the following variables: smoking, WEE,  ·VO2max, body mass index (BMI, waist-to-hip ratio (WHR, fat mass (FM, fat-free mass (FFM, lipid profile, hsCRP, glucose and insulin concentration, and insulin resistance. Between the subgroups created on the basis of hsCRP concentration, in quartiles 1 to 3 and quartile 4, a comparative analysis was carried out. 79.5�0of women and 69.3�0of men had hsCRP values within the references ranges. Moderately high values were found in 14.1�0of women and 22.7�0of men and high in 6.4�0and 7.9�20respectively. Mean values of BMI, FFM, WHR, WEE,  ·VO2max, glucose and triglyceride concentration, and TC/HDL index were significantly lower, while FM and HDL were significantly higher, in women than in men. In the quartile 4 subgroup compared to the quartile 1-3 subgroup, we found significantly lower HDL concentration and a tendency for higher values of BMI (p=0.06 and TC (p=0.07 as well as higher percentages of smoking among men. In young, physically active, healthy persons, serum concentration of hsCRP is not related to physical activity or  ·VO2max.

  18. KIMS, CEDIA, and HS-CEDIA immunoassays are inadequately sensitive for detection of benzodiazepines in urine from patients treated for chronic pain.

    Science.gov (United States)

    Darragh, Alicia; Snyder, Marion L; Ptolemy, Adam S; Melanson, Stacy

    2014-01-01

    Patients treated for chronic pain may frequently undergo urine drug testing to monitor medication compliance and detect undisclosed prescribed or illicit drug use. Due to the increasing use and abuse of benzodiazepines, this class of medications is often included in drug screening panels. However, immunoassay-based methods lack the requisite sensitivity for detecting benzodiazepine use in this population primarily due to their poor cross-reactivity with several major urinary benzodiazepine metabolites. A High Sensitivity Cloned Enzyme Donor Immunoassay (HS-CEDIA), in which beta-glucuronidase is added to the reagent, has been shown to perform better than traditional assays, but its performance in patients treated for chronic pain is not well characterized. To determine the diagnostic accuracy of HS-CEDIA, as compared to the Cloned Enzyme Donor Immunoassay (CEDIA) and Kinetic Interaction of Microparticles in Solution (KIMS) screening immunoassays and liquid chromatography-tandem mass spectrometry (LC-MS/MS), for monitoring benzodiazepine use in patients treated for chronic pain. A study of the diagnostic accuracy of urine benzodiazepine immunoassays. The study was conducted at an academic tertiary care hospital with a clinical laboratory that performs urine drug testing for monitoring medication compliance in pain management. A total of 299 urine specimens from patients treated for chronic pain were screened for the presence of benzodiazepines using the HS-CEDIA, CEDIA, and KIMS assays. The sensitivity and specificity of the screening assays were determined using the LC-MS/MS results as the reference method. Of the 299 urine specimens tested, 141 (47%) confirmed positive for one or more of the benzodiazepines/metabolites by LC-MS/MS. All 3 screens were 100% specific with no false-positive results. The CEDIA and KIMS sensitivities were 55% (78/141) and 47% (66/141), respectively. Despite the relatively higher sensitivity of the HS-CEDIA screening assay (78%; 110

  19. 基线hs-CRP和LDL-C水平与不同急性冠状动脉综合征急性期预后的相关性及意义%Correlation and significance of baseline hs-CRP and LDL-C levels with acute coronary syndrome prognosis

    Institute of Scientific and Technical Information of China (English)

    曹春辉; 黄军; 王仲华; 方永祥; 张晓红

    2012-01-01

    目的 探讨基线高敏C-反应蛋白(hs-CRP)和低密度脂蛋白胆固醇(LDL-C)水平与急性冠状动脉综合征(ACS)急性期预后的相关性及意义.方法 选取某院98例冠状动脉综合征患者,所有患者入院24h内测定基线状态的hs-CRP和LDL-C水平,治疗30 d后,按出现死亡、事件和无事件将患者分成3组,将3组患者基线hs-CRP和LDL-C水平进行比较分析,并对死亡组和事件组包括性别、年龄、hs-CRP、LDL-C在内的多项危险因素进行多因素Logistic回归分析,判断各因素对死亡率和事件发生率的影响.结果 治疗30 d后,死亡组的基线hs-CRP水平(61.3±35.1)显著高于事件组(35.2±17.3)和无事件组(11.9±5.2),P<0.05;死亡组的基线LDL-C水平(3.5±1.2)与事件组(3.4±2.1)和无事件组(3.1±1.8),差异无统计学意义,P>0.05;Logistic回归分析发现,基线hs-CRP水平(OR=3.126)、ST异常抬高(OR=2.831)、LVEF(OR=2.143)、Cr水平(OR=2.809)、糖尿病(OR=3.219)等是影响死亡的危险因素,P<0.05;高血压(OR=2.723)、中风史(OR=2.185)、吸烟(OR=2.139)、基线hs-CRP水平(OR=2.536)、ST异常抬高(OR=2.941)、LVEF(OR=2.735)、Cr水平(OR=3.023)是影响血管事件的危险因素,P<0.05;事件组和无事件组的hs-CRP水平较治疗前均显著降低,P<0.05;两组LDL-C水平治疗后较治疗前均略有降低,差异无统计学意义,P>0.05.结论 ACS患者急性期死亡和事件与hs-CRP水平明显相关,但与基线LDL-C水平无关.治疗后Hs-CRP水平明显降低,可作为急性期治疗的靶目标.%OBJECTIVE To investigate the correlation and significance for baseline high-sensitivity C-reactive protein (hs-CRP) and low-density lipoprotein cholesterol ( LDL-C) levels in the prognosis of patients with acute coronary syndrome (ACS). METHODS 98 cases of coronary syndrome patients in our hospital were chosen. The level of baseline hs-CRP and LDL-C of all patients in our hospital within 24 hours was measured. The determination of

  20. Analysis of Changes of Serum Hs-CRP and Myocardial Enzyme Before and After Treatment of Chronic Cardiopulmonary Disease%慢性肺心病治疗前后的血清hs-CRP及心肌酶水平等变化分析

    Institute of Scientific and Technical Information of China (English)

    陈芸; 王鹏; 李东敏

    2016-01-01

    Objective To investigate the changes of serum high-sensitivity C-reactive protein, myocardial enzyme and B-type natriuretic peptide of patients in the acute attack stage and remission stage of chronic cardiopulmonary disease. Methods 120 patients with chronic cardiopulmonary disease were selected, including 60 cases in the acute attack stage and 60 cases in the remission stage, and serum hs-CRP and myocardial enzymes of those patients were detected. 60 cases of healthy cases were selected into the control group. Results Hs-CRP, BNP, CK-MB, CK, LDH, AST levels of patients with chronic cardiopulmonary disease were signifi-cantly higher than those of the healthy patients in the control group, the levels of those indexes in the acute attack stage remarkably higher than those in the remission period (P<0. 01). After treatment, hs-CRP, BNP and myocardial enzyme levels of patients in the acute attack stage decreased significantly (P<0. 01). Conclusion Serum hs-CRP and myocardial enzyme levels obviously increases in the patients with chronic cardiopulmonary disease, especially in the acute attack stage, thus it can be regarded as an important indi-cator in the emergency, development and curative effect judgment of this disease.%目的:探讨慢性肺心病急性发作期及治疗缓解期的血清超敏C反应蛋白、心肌酶及血清B型钠尿肽的变化。方法选取120例慢性肺心病患者,其中急性发作期60例,缓解期60例,检测其血清hs-CRP及心肌酶,并以60例健康体检者作为对照。结果慢性肺心病患者的hs-CRP、 BNP、 CK-MB、 CK、 LDH、 AST水平显著高于健康对照组,而急性发作期则明显高于缓解期P<0.01。经治疗,急性发作期患者的hs-CRP、 BNP及心肌酶水平均明显下降P<0.01。结论慢性肺心病患者血清hs-CRP、心肌酶水平显著升高,尤其是急性发作期,故可作为预测慢性肺心病产生、进展及疗效判断的重要指标。