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Sample records for hicks ny dept

  1. Hicks on reality and time

    DEFF Research Database (Denmark)

    Madsen, Mogens Ove

    2010-01-01

    can be reduced into scientific terms is rather limited – as economics pushes on beyond statics it becomes less like science, and more like history. A further development of economic theory is required and like Post-keynesians Hicks in his later writings tries to find different ways to carry...

  2. Ultrasound, normal placenta - Braxton Hicks (image)

    Science.gov (United States)

    ... performed at 17 weeks gestation. It shows the placenta during a normal (Braxton Hicks) contraction. Throughout the ... contracts to facilitate better blood flow through the placenta and the fetus. In this ultrasound, the placenta ...

  3. FOREWORD: Dr Trevor J Hicks Dr Trevor J Hicks

    Science.gov (United States)

    Goossens, Darren

    2009-03-01

    This issue of Journal of Physics: Condensed Matter has been assembled to recognize the valuable contribution of Dr Trevor J Hicks to the field of neutron scattering and magnetism. Trevor began his study of magnetism as a PhD student at Monash University in Melbourne in the early 1960s, working with Professor Jack Smith. From the very beginning magnetism in alloys, and disordered systems in general, became a key aspect of his career. After a postdoctoral position at Harwell working with Dr Graeme Low Trevor returned to Australia and took up a position with Monash. He soon became a key figure in developing the capability for neutron scattering using the HIFAR reactor at the Australian Atomic Energy Commission, now the Australian Nuclear Science and Technology Organisation, ANSTO. The instrumentation was always developed to further his studies of magnetism. The development of polarization analysis measurements of diffuse magnetic scattering, first using iron filters and then his own design of supermirror benders for beam polarization, took place through the 1970s, 1980s and into the 1990s. Throughout this time, Trevor mentored a series of PhD students and postdoctoral fellows, many of whom have contributed to this issue (and, indeed, guest edited it). As befits a scientist and university academic for whom teaching has always been important, Trevor has not only created a strong body of significant research, he has also made a major contribution to preparing several generations of neutron scattering scientists, and this issue reflects that. When I approached Journal of Physics: Condensed Matter with a proposal for an issue in honour of Trevor, the response was immediate and positive. It is with great pleasure that I present the result of that proposal. The great diversity of the content, all centred on neutron scattering and magnetism, reflects the breadth of Trevor's own career and of the scientists with whom he has interacted. Finally, I would like to make some

  4. A Memory-Based Model of Hick's Law

    Science.gov (United States)

    Schneider, Darryl W.; Anderson, John R.

    2011-01-01

    We propose and evaluate a memory-based model of Hick's law, the approximately linear increase in choice reaction time with the logarithm of set size (the number of stimulus-response alternatives). According to the model, Hick's law reflects a combination of associative interference during retrieval from declarative memory and occasional savings…

  5. 10. O Olawoyin John Hick Pluralism in the Traditional Yoruba ...

    African Journals Online (AJOL)

    REGINALDS

    viz. to provide a theoretical basis for peaceful coexistence among different religions in. Nigeria. ... John Hick's Philosophy of Religious Pluralism in the Context of Traditional Yoruba Religion 87 ... The present article makes such an attempt in the ..... Man really finds little satisfaction except in a Deity who lives, who has.

  6. Kuraator Alistair Hicks: kuidas õppida pilte usaldama? / intervjueerinud Tanel Veenre

    Index Scriptorium Estoniae

    Hicks, Alistair

    2011-01-01

    Fotokuu raames Eestit külastanud Deutsche Banki kunstikollektsiooni üks kuraatoreid Alistair Hicks huvist foto vastu kunstiturul, Deutsche Banki kollektsioonist, mis sisaldab kunsti paberil, praegusest fotost, eesti fotograafi Sigrid Viiri skulptuurina esitletud fotodest. Panga kollektsioonis on Krista Mölderi fotod seeriast "Landmarks"

  7. UTEROPLACENTAL DOPPLER VELOCIMETRY DURING BRAXTON-HICKS CONTRACTIONS

    NARCIS (Netherlands)

    DIJKSTRA, K; AARNOUDSE, JG; Oosterhof, H.

    1992-01-01

    Pulsed-wave Doppler ultrasound was used to evaluate the influence of Braxton Hicks' contractions on flow velocity waveforms in the uterine arteries. Flow velocity waveforms were obtained from a standardized site, at the crossing of the uterine artery with the external iliac artery near the uterine w

  8. Hayek, Hicks e a ascensão do walrasianismo Hayek, Hicks and the rise of walrasianism

    Directory of Open Access Journals (Sweden)

    Jorge Eduardo de Castro Soromenho

    2012-08-01

    Full Text Available O artigo discute a origem e a evolução dos conceitos de equilíbrio e tempo que Hicks utilizou para interpretar a Teoria Geral de Keynes. O trabalho está organizado em duas partes. Na primeira, analisaram-se os trabalhos de Hayek. Examinaram-se sua concepção de equilíbrio intertemporal e projeto de desenvolver uma teoria dos ciclos. Por último, discutiram-se a implementação desse projeto e seus problemas. Na segunda parte, foi examinado como Hicks, ao refletir sobre os problemas da teoria de Hayek, desenvolveu seus próprios conceitos de equilíbrio e tempo.In this article, the origins and evolution of the equilibrium and time concepts that Hicks later used to interpret Keynes' theory are discussed. The paper is organized into two parts. In the first, the work of Hayek is analyzed, examining his conception of intertemporal equilibrium, his project to develop a theory of cycles, and lastly, the implementation of this project in Prices and Production and the related problems. In the second part we examine how Hicks, reflecting on the problems raised by Hayek´s theory, developed his own concepts of equilibrium and time.

  9. As primeiras impressões de Hicks sobre a Teoria Geral

    Directory of Open Access Journals (Sweden)

    Jorge Eduardo de Castro Soromenho

    2014-06-01

    Full Text Available In this article, we present an interpretation of the Hicks' first review of Keynes's General Theory. First we analyze the influence of Hayek's theory on the work of Hicks. We show how Hicks, reflecting on the Austrian theory of the business cycle, created the concepts of "week" and temporary equilibrium. Then we discuss how the author reformulated these concepts of time and equilibrium to interpret the General Theory.

  10. Exceptions to Hick's law: explorations with a response duration measure.

    Science.gov (United States)

    Longstreth, L E; el-Zahhar, N; Alcorn, M B

    1985-12-01

    Five experiments used a new response-duration measure in explorations of the conditions necessary for confirmation of Hick's law. Hick's law states that reaction time increases logarithmically with number of choices. Exceptions to the law, venerable as it is, have been reported. They have always included the following conditions: a verbal response; a familiar stimulus with a single dominant name; and a large number of practice trials. These conditions have carried a heavy explanatory burden in accounting for the anamolous results. The present studies use none of these conditions and yet manage to replicate the anamolous result of a very shallow slope across set size, a slope less than one-tenth the usual value. This was accomplished by using a novel task in which the initial component of the response is the same for all stimuli (depression of a single response key) but the termination of the response is different (different durations for each stimulus). Using this task, a slope in the neighborhood of 15 ms per bit of stimulus uncertainty is found, as compared with the usual value of about 150 ms. A number of possible explanations are examined. Among the most important are the possibilities that response overlap is the critical factor (i.e., duration errors overlap); possible stimuli are simply ignored when more than one is involved; and the duration decision is made after the reaction-time interval rather than during it. All three possibilities, as well as some others, are found to be inconsistent with the various experimental outcomes. Instead, a new theory of choice reaction time is presented, which emphasizes the nature of the S-R code that is assumed to represent various reaction-time tasks. This theory leads to a new "law" that is put forward as a replacement for Hick's law. It is RT = a + b(1 - N-1).

  11. Porridge and peas: C. Stanton Hicks and Australian army rations.

    Science.gov (United States)

    Collingham, Lizzie

    2009-09-01

    In 1942 Australian troops came back from fighting the Japanese in New Guinea exhausted and malnourished. The army rations of bully beef and biscuits were insufficiently rich in vitamins to sustain men in combat in tropical conditions. The nutritionist C. Stanton Hicks was one of a vast army of scientists who worked behind the scenes to maximize the war effort. He made it his mission to improve the army diet. He set up the Australian Army Catering Corps, invented combat ration packs and tried to introduce vitamin-rich foods into the soldiers' diet. Two of his more idiosyncratic innovations were wheat porridge and Tasmanian blue peas.

  12. FETAL DOPPLER VELOCIMETRY IN THE INTERNAL CAROTID AND UMBILICAL ARTERY DURING BRAXTON-HICKS CONTRACTIONS

    NARCIS (Netherlands)

    DIJKSTRA, K; AARNOUDSE, JG; Oosterhof, H.

    1992-01-01

    Using Doppler ultrasound, previous studies revealed a considerable increase in vascular resistance in the uteroplacental circulation during Braxton Hicks' contractions. Consequently, uteroplacental blood flow is reduced and this affects placental oxygen transfer to the fetus, causing a fall in fetal

  13. La crítica de Hicks al Tratado del Dinero de Keynes

    Directory of Open Access Journals (Sweden)

    Alexander Tobón

    2004-12-01

    Full Text Available El objetivo de este artículo es mostrar que la crítica que hace Hicks al Tratado del Dinero de Keynes es incorrecta. Para ello, presentamos el modelo de Keynes, mostrando que la igualdad entre la inversión y el ahorro es una condición de equilibrio monetario y no una identidad. Este resultado no puede ser obtenido en el análisis de Hicks.

  14. Hick Samuelson Keynes Dynamic Economic Model with Discrete Time and Consumer Sentiment

    Science.gov (United States)

    Dobrescu, Loretti I.; Neamå#U, Mihaela; Opriş, Dumitru

    The paper describes the Hick Samuelson Keynes dynamical economic model with discrete time and consumer sentiment. We seek to demonstrate that consumer sentiment may create fluctuations in the economical activities. The model possesses a flip bifurcation and a Neimark-Sacker bifurcation, after which the stable state is replaced by a (quasi-) periodic motion.

  15. Landscape architecture student Christina Hicks receives recognition in James River Green Building Council's 2009 Green Spaces Competition

    OpenAIRE

    Chadwick, Heather Riley

    2009-01-01

    Christina Hicks of Stafford, Va., a fifth-year landscape architecture student, is the Honor Winner of the 2009 James River Green Building Council's (JRGBC) Green Spaces Competition in the student division.

  16. La dinámica en economía. Los enfoques de Hicks y Samuelson

    OpenAIRE

    1995-01-01

    Este artículo compara las concepciones de Hicks y Samuelson acerca de la dinámica. Para el primero, los análisis dinámicos deben partir de una definición de del período. El segundo, por su parte, asocia los procesos dinámicos a relaciones de vectores de fuerza, como en la mecánica clásica.

  17. A Note on Discrete Multitime Recurrences of Samuelson-Hicks Type

    Directory of Open Access Journals (Sweden)

    Bruno Antonio Pansera

    2016-01-01

    Full Text Available By this work, we aim at fostering further research on the applications of multitime recurrences. In particular, we shall apply this method by generalizing the Samuelson-Hicks model so as to make the new concept of time that this method proposes clear. In particular, the multitime approach decomposes a point of time into a vector, taking into account how different coordinates of time referring to the same date can affect the dynamics of a model.

  18. La dinámica en economía. Los enfoques de Hicks y Samuelson

    Directory of Open Access Journals (Sweden)

    Pecha Arcenio

    1995-12-01

    Full Text Available Este artículo compara las concepciones de Hicks y Samuelson acerca de la dinámica. Para el primero, los análisis dinámicos deben partir de una definición de del período. El segundo, por su parte, asocia los procesos dinámicos a relaciones de vectores de fuerza, como en la mecánica clásica.

  19. Different (key)strokes for different folks: How standard and nonstandard typists balance Fitts' law and Hick's law.

    Science.gov (United States)

    Logan, Gordon D; Ulrich, Jana E; Lindsey, Dakota R B

    2016-12-01

    Fine motor skills like typing involve a mapping problem that trades Fitts' law against Hick's law. Eight fingers have to be mapped onto 26 keys. Movement time increases with distance, so Fitts' law is optimized by recruiting more fingers. Choice difficulty increases with the number of alternatives, so Hick's law is optimized by recruiting fewer fingers. The effect of the number of alternatives decreases with consistent practice, so skilled typists achieve a balance between Fitts' law and Hick's law through learning. We tested this hypothesis by comparing standard typists who use the standard QWERTY mapping consistently with nonstandard typists who use fewer fingers less consistently. Typing speed and accuracy were lower for nonstandard typists, especially when visual guidance was reduced by removing the letters from the keys or covering the keyboard. Regression analyses showed that accommodation to Fitts' law (number of fingers) and Hick's law (consistency) predicted typing speed and accuracy. We measured the automaticity of typing in both groups, testing for hierarchical control in 3 tasks: word priming, which measures parallel activation of keystrokes, keyboard recall, which measures explicit knowledge of letter locations, and hand cuing, which measures explicit knowledge of which hand types which letter. Standard and nonstandard typists showed similar degrees of hierarchical control in all 3 tasks, suggesting that nonstandard typists type as automatically as standard typists, but their suboptimal balance between Fitts' law and Hick's law limits their ability to type quickly and accurately. (PsycINFO Database Record

  20. On Sequence Learning Models: Open-loop Control Not Strictly Guided by Hick's Law.

    Science.gov (United States)

    Pavão, Rodrigo; Savietto, Joice P; Sato, João R; Xavier, Gilberto F; Helene, André F

    2016-03-15

    According to the Hick's law, reaction times increase linearly with the uncertainty of target stimuli. We tested the generality of this law by measuring reaction times in a human sequence learning protocol involving serial target locations which differed in transition probability and global entropy. Our results showed that sigmoid functions better describe the relationship between reaction times and uncertainty when compared to linear functions. Sequence predictability was estimated by distinct statistical predictors: conditional probability, conditional entropy, joint probability and joint entropy measures. Conditional predictors relate to closed-loop control models describing that performance is guided by on-line access to past sequence structure to predict next location. Differently, joint predictors relate to open-loop control models assuming global access of sequence structure, requiring no constant monitoring. We tested which of these predictors better describe performance on the sequence learning protocol. Results suggest that joint predictors are more accurate than conditional predictors to track performance. In conclusion, sequence learning is better described as an open-loop process which is not precisely predicted by Hick's law.

  1. Nye Medier - ny journalistik?

    DEFF Research Database (Denmark)

    Hartley, Jannie Møller

    2009-01-01

    På baggrund af en pilotundersøgelse undersøger denne artikel forskningens hypoteser om netjournalistikken, med særligt fokus på den nordiske litteratur på området. Det argumenteres, at der med netmediet og netavisernes vækst ikke nødvendigvis er tale om ny journalistik; på den ene side bekræfter ...... nogle traditionelle journalistiske rutiner i nyhedsproduktionen.En radikalisering der indikerer, at nyheden i den digitale tidsalder kan ses som en dynamisk proces frem for et statisk produkt. Udgivelsesdato: 2009...

  2. Faglighed + test = ny elevtype

    DEFF Research Database (Denmark)

    Drejer, Claus Munch

    2010-01-01

    Kronikken handler om tiden efter den seneste folkeskolelov, samt de teknikker der indføres i bestræbelserne på at optimere elevernes færdigheder og kundskaber. Siden de dårlige PISA-test i 2000, og specielt 2003, har der været fokus på det kernefaglige, og for at sikre eleverne denne faglighed in...... indføres en række teknikker såsom evaluering, test, trin- og slutmål, der skal sikre at eleverne optimere deres faglighed. Dette peger frem mod en ny elevtype - en præstationsorienteret elev....

  3. Tendenser i ny tysk litteratur

    DEFF Research Database (Denmark)

    Povlsen, Steen Klitgård

    Arbejdspapiret indeholder to foredrag: dels om ny tysk litteraturs forhold til 2. verdenskrig, dels om Pater Handke og hans roman "Don Juan (fortalt af ham selv)......Arbejdspapiret indeholder to foredrag: dels om ny tysk litteraturs forhold til 2. verdenskrig, dels om Pater Handke og hans roman "Don Juan (fortalt af ham selv)...

  4. Trails, Snowmobile, Available from Parks Dept., Published in unknown, Sauk County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Trails, Snowmobile dataset, was produced all or in part from Hardcopy Maps information as of unknown. It is described as 'Available from Parks Dept.'. Data by...

  5. J-refocused 1H PRESS DEPT for localized 13C MR spectroscopy.

    Science.gov (United States)

    Chen, X; Boesiger, P; Henning, A

    2013-09-01

    Proton point-resolved spectroscopy (PRESS) localization has been combined with distortionless enhanced polarization transfer (DEPT) in multinuclear MRS to overcome the signal contamination problem in image-selected in vivo spectroscopy (ISIS)-combined DEPT, especially for lipid detection. However, homonuclear proton scalar couplings reduce the DEPT enhancement by modifying the spin coherence distribution under J modulation during proton PRESS localization. Herein, a J-refocused proton PRESS-localized DEPT sequence is presented to obtain simultaneously enhanced and localized signals from a large number of metabolites by in vivo (13) C MRS. The suppression of J modulation during PRESS and the substantial recovery of signal enhancement by J-refocused PRESS-localized DEPT were demonstrated theoretically by product operator formalism, numerically by the spin density matrix simulations for different scalar coupling conditions, and experimentally with a glutamate phantom at various TEs, as well as a colza oil phantom. The application of the sequence for localized detection of saturated and unsaturated fatty acids in the calf bone marrow and skeletal muscle of healthy subjects yielded high signal enhancements simultaneously obtained for all components.

  6. Critical comment on Hicks-Caskey and Potter, "Effect of the full moon on a sample of developmentally delayed, institutionalized women".

    Science.gov (United States)

    Flynn, M

    1991-12-01

    Hicks-Caskey and Potter (1991) claim to have found a "full moon effect" on women in a developmental center. Further, they suggest the discrepancies in findings on lunar effects can be accounted for by (i) a lack of equivalent operational definitions and (ii) a person selection factor. It is argued that the Hicks-Caskey and Potter findings are undermined by weekday, holiday, season, weather, particular staff-subject interactions, and expectancy effects. In addition, the proposed explanations for differing outcomes in lunar studies do not explain both the negative findings and conflicting positive findings.

  7. Studerende giver ny indsigt til digital byudvikling

    DEFF Research Database (Denmark)

    2016-01-01

    http://studerende.au.dk/studier/fagportaler/arts/aktuelt/nyheder/vis-nyhed/artikel/studerende-giver-ny-indsigt-til-digital-byudvikling/......http://studerende.au.dk/studier/fagportaler/arts/aktuelt/nyheder/vis-nyhed/artikel/studerende-giver-ny-indsigt-til-digital-byudvikling/...

  8. Udlejers godkendelse af ny erhvervslejer

    DEFF Research Database (Denmark)

    Munk-Hansen, Carsten

    2009-01-01

    I artiklen er taget udgangspunkt i den »klassiske« afståelsessituation, hvor en lejer vil afhænde den i de lejede lokaler drevne virksomhed, og lejeren har afståelsesret til en af udlejer godkendt lejer, uden at kravene til den ny lejer er specificeret. På baggrund af Vestre Landsrets dom af 9/2 ...

  9. Ny industrialisering - et bedre produktmarked

    DEFF Research Database (Denmark)

    Beim, Anne

    2001-01-01

    for byggeriet med titlerne: 1. Bygherren som forandringsagent. 2. Tæt samarbejde i byggedelen. 3. Ny industrialisering. 4. Byg med kommunikation og innovation. Dette debathæfte er nummer tre i rækken og omhandler byggeriets industridel og mulighederne for at fremme en succesfuld industriel produktion....

  10. Home and away - Raymond Pettibon and Marko Mäetamm / Alistair Hicks, Elo-Hanna Seljamaa, Marko Mäetamm, Liina Siib

    Index Scriptorium Estoniae

    2015-01-01

    Vestluringis on näituse „Kodus ja võõrsil. Raymond Pettibon: Ameerika unelmas elades. Marko Mäetamm: Tunne end nagu kodus” kuraator Alistair Hicks, Marko Mäetamm, Elo-Hanna Seljamaa ja Liina Siib

  11. Home and away - Raymond Pettibon and Marko Mäetamm / Alistair Hicks, Elo-Hanna Seljamaa, Marko Mäetamm, Liina Siib

    Index Scriptorium Estoniae

    2015-01-01

    Vestluringis on näituse „Kodus ja võõrsil. Raymond Pettibon: Ameerika unelmas elades. Marko Mäetamm: Tunne end nagu kodus” kuraator Alistair Hicks, Marko Mäetamm, Elo-Hanna Seljamaa ja Liina Siib

  12. Commentary: Science, Technology, and Society in Guidelines for Using Technology to Prepare Social Studies Teachers--A Reply to Hicks et al. and Crocco and Leo

    Science.gov (United States)

    Mason, Lance

    2015-01-01

    This essay is a response to both the "Guidelines for Using Technology to Prepare Social Studies Teachers" published in this journal by Hicks, Lee, Berson, Bolick, and Diem (2014) and the rejoinder by Crocco and Leo (2015). The author agrees with Crocco and Leo's assessment that removing the principal regarding science, technology, and…

  13. Urban metabolism in Syracuse, NY – introduction

    Science.gov (United States)

    David J. Nowak

    2016-01-01

    This special issue of Urban Ecosystems contains a series of papers related to assessing urban metabolism in Syracuse, NY. These papers were developed under the Urban Long-Term Research Area Exploratory Awards Program funded by the National Science Foundation. Objectives of this two-year project (2009–2011) in Syracuse, NY were to investigate: a)...

  14. Urine antibody against human cancer antigen NY-ESO-1

    OpenAIRE

    Jäger, Dirk; Stockert, Elisabeth; Karbach, Julia; Herrlinger, Kristina; Atmaca, Akin; Arand, Michael; Chen, Yao-Tseng; Gnjatic, Sacha; Old, Lloyd J.; Knuth, Alexander; Jäger, Elke

    2002-01-01

    NY-ESO-1 is one of the most immunogenic tumor antigens known to date. Spontaneous humoral and cellular immune responses against NY-ESO-1 are detected in a substantial proportion of patients with NY-ESO-1 positive cancers. NY-ESO-1 serum antibody is dependent on the presence of NY-ESO-1+ cancer cells, and antibody titers correlate with the clinical development of the disease. NY-ESO-1 serum antibody is associated with detectable NY-ESO-1-specific CD8+ T cell reactivity. High titers of NY-ESO-1...

  15. Sir John Hicks a la reconquista de la dicotomía clásica: a propósito de IS-LM

    Directory of Open Access Journals (Sweden)

    González Jorge Iván

    1987-06-01

    Full Text Available

    Sir John Hicks, el caballero excelso de la armada bastarda, ha luchado desde 1937 por mostrar que la obra de Keynes, especialmente la Teoría General, no constituyó un rompimiento con el pensamiento tradicional. Gracias a la tenacidad de quien fuera ungido caballero en 1964, la síntesis neoclásica ha logrado sobrevivir a las críticas demoledoras de autores como Leijonhuvfud, Davidson, Weintraub, Uzawa, etc.

  16. Multikulturalisme er en ny slags Apartheid

    DEFF Research Database (Denmark)

    Dyrberg, Torben Bech

    2016-01-01

    I en ny antologi retter forfatterne et kritisk lys mod forsvarerne af multikulturalisme. Og det er der god grund til, mener en af bogens redaktører. For den store gruppe, der hylder multikulturen, glemmer i al dens godhed, at de samtidig er med til at undergrave demokratiet og styrke uheldige mag...

  17. Multikulturalisme er en ny slags Apartheid

    DEFF Research Database (Denmark)

    Dyrberg, Torben Bech

    2016-01-01

    I en ny antologi retter forfatterne et kritisk lys mod forsvarerne af multikulturalisme. Og det er der god grund til, mener en af bogens redaktører. For den store gruppe, der hylder multikulturen, glemmer i al dens godhed, at de samtidig er med til at undergrave demokratiet og styrke uheldige mag...

  18. Mangfoldighed som ny EU-politik

    DEFF Research Database (Denmark)

    Thomsen, Steen

    2014-01-01

    Analyse. EU er stadig i krise. EU er endnu ikke kommet med i det globale opsving efter finanskrisen, og Sydeuropas strukturproblemer er uløste, men maskeret af statsstøtte via ECB. Vælgerne gav EU-politikerne en alvorlig advarsel ved valget til Europa-Parlamentet. Efter en bølge af ny regulering er...

  19. 2008 FEMA Lidar: South Oneida County (NY)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — For Oneida County, NY, there were two types of elevation datasets. The first type is LiDAR and the second one is Auto-correlation DEM. Auto-correlation DEM data was...

  20. La teoria monetaria e la determinazione dei prezzi fissi e flessibili: una valutazione di A Market Theory of Money di John Hicks

    Directory of Open Access Journals (Sweden)

    J.A. KREGEL

    2013-10-01

    Full Text Available The work represents an analysis of Sir John Hick’s book A market theory of money. Sir John has, on a number of occasions, taken his distance from the SI-LL model and what has been made of it in mainstream economics. He has never done this with respect to his own approach to money, or discussed why, given the initial similarity with Keynes’ approach to liquidity, he went on to develop a diverse approach. It is this difference which will be the centre of our discussion.  JEL Codes: B22, B31, E30Keywords: John Hicks, A market theory of money, SI-LL

  1. Mot en ny pedagogisk lederrolle og lederidentitet?

    Directory of Open Access Journals (Sweden)

    Ann Kristin Larsen

    2014-02-01

    Full Text Available Artikkelen omhandler hvordan endrede ledelsesstrukturer oppstått gjennom ny organisasjonsmodell virker inn på de pedagogiske ledernes arbeidsoppgaver. Vi har etterspurt deres erfaringer knyttet til mulige endringer i oppgaver, arbeidsdeling og faglig-pedagogisk fokus i arbeidet. Undersøkelsen ble gjennomført i en bydel i Oslo. Hovedfunnene er at pedagogiske ledere har fått flere administrative oppgaver og personalleder oppgaver. De pedagogiske lederne deler seg i to med hensyn til hvor fornøyde de er med disse endringene. I artikkelen drøfter vi hva denne todelingen betyr for deres rolleforståelse og identitet.

  2. 33 CFR 80.160 - Montauk Point, NY to Atlantic Beach, NY.

    Science.gov (United States)

    2010-07-01

    ... Light 322° true across the southwest tangent of the island on the north side of Jones Inlet to the... to Atlantic Beach, NY. (a) A line drawn from the Shinnecock Inlet East Breakwater Light to Shinnecock Inlet West Breakwater Light 1. (b) A line drawn from Moriches Inlet East Breakwater Light to...

  3. Faget almen dannelse og ny teknologi

    DEFF Research Database (Denmark)

    Tiemensma, Britt Due

    2015-01-01

    16 lektioner anvendes Skoletube, en digital platform rettet mod folkeskolen samt gratis programmer, som jeg forestiller mig vil imødekomme de studerendes behov for sjove oplevelser og samtidig ruste dem til at forholde sig reflekteret og nuanceret til etiske, politiske, demokratiske og religiøse......Denne case handler om, hvordan 90 lærerstuderende på 1. årg. introduceres til faget almen dannelse ud fra en antagelse om, at ny teknologi påvirker forestillingen om, hvad der er relevant viden for professionsudøvelsen, og således også hvad der skal prioriteres i studiet. I introduktionsforløbet på...... udfordringer i relation til skole- og forældresamarbejde. Udfordringen er altså at få ny teknologi til at indgå som aktør i faget almen dannelse og skabe en eksemplarisk praksis, hvor de studerende kan udvikle sig fagligt og personligt i forhold til kommende undervisningsopgaver i folkeskolen....

  4. A contribuição de John Hick para o diálogo inter-religioso: a leitura do evento crístico como metáfora

    Directory of Open Access Journals (Sweden)

    Rodrigo Portella

    2009-05-01

    Full Text Available Este é um comentário crítico do livro A metáfora do Deus encarnado (2000, de John Hick. Hick busca rever axiomas cristológicos, tentando reinterpretá-los no intuito de fazê-los significativos para o diálogo inter-religioso. Propõe uma cristologia pluralista, desconstruindo tradicionais premissas e dando novos significados ao evento Jesus. A partir de uma nova cristologia, oferece uma soteriologia que transcende o evento crístico.Palavras-chave: Cristologia; Soteriologia; Diálogo interreligioso; Teologia contemporânea.ABSTRACTThis is a critical review of John Hick’s book The metaphor of red God (2000. Hick reviews Christological axioms, attempting to reinterpret them so as to render them significant to the dialogue between religions. He proposes a pluralist Christology, deconstructing traditional premises and investing Jesus’ event with new meanings. With basis on a new Christology, he offers a soteriology that transcends Christ’s event.Key words: Christology; Soteriology; Dialogue between religions; Contemporary theology.

  5. 33 CFR 110.208 - Buffalo Harbor, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Buffalo Harbor, N.Y. 110.208 Section 110.208 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.208 Buffalo Harbor, N.Y. (a) The anchorage...

  6. 33 CFR 110.84b - Buffalo, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Buffalo, N.Y. 110.84b Section 110.84b Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.84b Buffalo, N.Y. The area within the Port of Buffalo known as...

  7. NY-ESO-1 specific antibody and cellular responses in melanoma patients primed with NY-ESO-1 protein in ISCOMATRIX and boosted with recombinant NY-ESO-1 fowlpox virus.

    Science.gov (United States)

    Chen, Ji-Li; Dawoodji, Amina; Tarlton, Andrea; Gnjatic, Sacha; Tajar, Abdelouahid; Karydis, Ioannis; Browning, Judy; Pratap, Sarah; Verfaille, Christian; Venhaus, Ralph R; Pan, Linda; Altman, Douglas G; Cebon, Jonathan S; Old, Lloyd L; Nathan, Paul; Ottensmeier, Christian; Middleton, Mark; Cerundolo, Vincenzo

    2015-03-15

    Vaccination strategies based on repeated injections of NY-ESO-1 protein formulated in ISCOMATRIX particles (NY-ESO-1 ISCOMATRIX) have shown to elicit combined NY-ESO-1 specific antibody and T cell responses. However, it remains unclear whether heterologous prime-boost strategies based on the combination with NY-ESO-1 ISCOMATRIX with different NY-ESO-1 boosting reagents could be used to increase NY-ESO-1 CD8(+) or CD4(+) T cell responses. To address this question, we carried out a randomized clinical trial in 39 high-risk, resected melanoma patients vaccinated with NY-ESO-1 ISCOMATRIX, and then boosted with repeated injections of either recombinant fowlpox virus encoding full length NY-ESO-1 (rF-NY-ESO-1) (Arm A) or NY-ESO-1 ISCOMATRIX alone (Arm B). We have comprehensively analyzed NY-ESO-1 specific T cells and B cells response in all patients before and after vaccination for a total of seven time points per patient. NY-ESO-1 ISCOMATRIX alone elicited a strong NY-ESO-1 specific CD4(+) T cell and antibody response, which was maintained by both regiments at similar levels. However, CD8(+) T cell responses were significantly boosted in 3 out of 18 patients in Arm A after the first rF-NY-ESO-1 injection and such responses were maintained until the end of the trial, while no patients in Arm B showed similar CD8(+) T cell responses. In addition, our results clearly identified immunodominant regions in the NY-ESO-1 protein: NY-ESO-179-102 and NY-ESO-1115-138 for CD4+ T cells and NY-ESO-185-108 for CD8+ T cells in a large proportion of vaccinated patients. These regions of NY-ESO-1 protein should be considered in future clinical trials as immunodominant epitopes. © 2014 UICC.

  8. Induction of primary NY-ESO-1 immunity: CD8+ T lymphocyte and antibody responses in peptide-vaccinated patients with NY-ESO-1+ cancers

    OpenAIRE

    Jäger, Elke; Gnjatic, Sacha; Nagata, Yasuhiro; Stockert, Elisabeth; Jäger, Dirk; Karbach, Julia; Neumann, Antje; Rieckenberg, Julia; Chen, Yao-Tseng; Ritter, Gerd; Hoffman, Eric; Arand, Michael; Old, Lloyd J.; Knuth, Alexander

    2000-01-01

    Cancer–testis antigen NY-ESO-1 is one of the most immunogenic tumor antigens defined to date. Spontaneous humoral and CD8+ T-cell responses to NY-ESO-1 are detected in 40–50% of patients with advanced NY-ESO-1-expressing tumors. A clinical trial was initiated to study the immunological effects of intradermal vaccination with 3 HLA-A2-binding NY-ESO-1 peptides in 12 patients with metastatic NY-ESO-1-expressing cancers. Seven patients were NY-ESO-1 serum antibody neg...

  9. Induction of primary NY-ESO-1 immunity: CD8+ T lymphocyte and antibody responses in peptide-vaccinated patients with NY-ESO-1+ cancers

    OpenAIRE

    2000-01-01

    Cancer–testis antigen NY-ESO-1 is one of the most immunogenic tumor antigens defined to date. Spontaneous humoral and CD8+ T-cell responses to NY-ESO-1 are detected in 40–50% of patients with advanced NY-ESO-1-expressing tumors. A clinical trial was initiated to study the immunological effects of intradermal vaccination with 3 HLA-A2-binding NY-ESO-1 peptides in 12 patients with metastatic NY-ESO-1-expressing cancers. Seven patients were NY-ESO-1 serum antibody neg...

  10. EnviroAtlas - New York, NY - Block Groups

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas dataset is the base layer for the New York, NY EnviroAtlas community. The block groups are from the US Census Bureau and are included/excluded based...

  11. 77 FR 16800 - Radio Broadcasting Services; Westfield, NY

    Science.gov (United States)

    2012-03-22

    ... From the Federal Register Online via the Government Publishing Office ] FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Westfield, NY AGENCY: Federal Communications..., Radio broadcasting. Federal Communications Commission. Nazifa Sawez, Assistant Chief, Audio...

  12. Middle Holocene Unconformity in Seneca Lake, NY

    Science.gov (United States)

    Curtin, T. M.; Crocker, M.; Loddengaard, K.

    2008-12-01

    The post-glacial history of the Finger Lakes, NY have involved several changes in lake levels throughout the latest Pleistocene and Holocene, resulting from the changing position of the retreating Laurentide ice sheet, river outlet position, glacial rebound, and water balance. Previous studies of high-resolution seismic reflection profiles from three Finger Lakes define a middle Holocene erosional surface at water depths as great as 26 m in the northern end of each of lake. There are two proposed hypotheses to explain the origin of the observed erosional surfaces: 1) subaerial erosion during a lake lowstand and 2) erosion resulting from increased internal seiche activity. To evaluate these hypotheses, we examined a series of 2 to 5 m long piston cores collected along a north-south transect from one of the Finger Lakes, Seneca Lake. Cores were correlated using distinctive changes in the profiles of grain size, loss-on-ignition, and magnetic susceptibility. We recognize a significant erosional unconformity of early to middle Holocene sediment at modern depths 60 m, the unconformity continues as a conformable zone. We attribute the unconformity to wave abrasion and nearshore current winnowing of the shoreface during a lowstand. With an assumption of an effective 20 m wave base, the depth to the low level lake surface responsible for the unconformity is estimated to be 40 m. The age of the unconformity is ~6 ka, based on radiocarbon ages of lithologic boundaries in the sediment cores. Because the unconformity grades into a conformable zone in deepwater cores that display no change in lithology, we hypothesize that the large-scale lake level drop is likely not the result of climate change, but rather a change in accommodation space in the northern portion of the lake basin due to glacial rebound.

  13. Autoimmune thyroid disease elicited by NY-ESO-1 vaccination.

    Science.gov (United States)

    Vita, Roberto; Guarneri, Fabrizio; Agah, Ravin; Benvenga, Salvatore

    2014-02-01

    Immunotherapies and targeted therapies are frequently associated with thyroid dysfunction, which is in contrast with the rare thyroid abnormalities induced by cytotoxic agents. Immunotherapy with NY-ESO-1, a tumor-associated antigen expressed by a number of malignancies, was reported to trigger hyperthyroidism or hypothyroidism in two HLA-A2 patients with ovarian cancer. We describe now a case of Graves' disease triggered by NY-ESO-1 in a HLA-A2-negative woman. A 32-year-old woman with a synovial sarcoma received radiotherapy, chemotherapy, and finally NY-ESO-1 vaccine. The patient was found to have HLA A11/A33(19), B13/B56(22), Cw3/-. One month after the beginning of immunotherapy, thyroid dysfunction was clinically suspected and Graves' disease was biochemically confirmed. Fearful of the antithyroid drugs' side effects, the patient was treated with a beta-blocker (propranolol, 80-20 mg/day). As hyperthyroidism progressively worsened, the patient underwent total thyroidectomy. We hypothesized that NY-ESO-1 shared partial homology with thyroid autoantigens (the so-called molecular mimicry mechanism) and that at least one pair of homologous sequences contained amino acid sequence binding motifs to a restricted number of HLA molecules. We used BLAST software to search amino acid sequence homologies between NY-ESO-1 and thyroid autoantigens (thyrotropin receptor [TSH-R], thyroperoxidase, and thyroglobulin), and the HLA ligand/motif database to look for HLA/T-cell receptor binding motifs in the regions of NY-ESO-1 and thyroid autoantigens that were homologous. We found 15 epitopic regions of NY-ESO-1 homologous to 15 regions of thyroid autoantigens, some of which epitopic: 5 of TSH-R, 8 of thyroglobulin, and 2 of thyroperoxidase. These homologous sequences contain binding motifs belonging to several HLA class I antigens, including HLA A2 and the patient's A11 and A33. Genetically predisposed patients who receive NY-ESO-1 vaccination are at risk to develop thyroid

  14. En ny generasjon troverdige kliniske retningslinjer

    Directory of Open Access Journals (Sweden)

    Per Olav Vandvik

    2013-11-01

    Full Text Available Trygg og effektiv diagnostikk, pleie og behandling fordrer at helsepersonell har tilgang til oppdatert og gyldig forskningsbasert kunnskap, fortrinnsvis i form av faglige kliniske retningslinjer utviklet med tilfredsstillende metodikk. De fleste retningslinjer har imidlertid betydelige svakheter med hensyn til både kunnskapsgrunnlag, utvikling av anbefalinger, presentasjonsformater og oppdatering. Nye definisjoner, standarder og metoder for kunnskapsbaserte troverdige retningslinjer gir langt bedre muligheter enn tidligere for å lykkes i arbeidet med å utvikle og implementere retningslinjer, men stiller samtidig større krav til metodekompetanse, klinisk ekspertise og tid. I vår digitale tidsalder åpner ny nettbasert teknologi for bedre disseminering av retningslinjer til frontlinjene i klinikken, og til å dele kunnskap med pasienter i konsultasjonen, for eksempel gjennom smarttelefoner og lesebrett. Gjennom et praktisk eksempel redegjør vi i denne artikkelen for nye definisjoner, standarder, metoder og verktøy for utvikling, disseminering og oppdatering av faglige retningslinjer. GRADE-systemet er en sentral komponent i dette. Det er nå mulig å lage troverdige retningslinjer som klinikere og pasienter kan ta i bruk i en travel hverdag. Det gjenstår å se hvordan myndighetene og fagmiljøene vil bidra for å sikre pasienter trygg og effektiv behandling gjennom utvikling av denne nye generasjonen av kliniske retningslinjer.Vandvik PO, Berg R, Vist G. A new generation of trustworthy clinical practice guidelines. Nor J Epidemiol 2013; 23 (2: 197-204.ENGLISH SUMMARYSafe and effective diagnosis and treatment requires that health care personnel can access the best current research evidence, preferably through clinical practice guidelines. Most guidelines suffer from methodological weaknesses (e.g., identification and assessment of research evidence, development of recommendations, suboptimal presentation formats and infrequent updating of

  15. 78 FR 45957 - Notice of Inventory Completion: Columbia University, Department of Anthropology, New York, NY

    Science.gov (United States)

    2013-07-30

    ..., Columbia University, New York, NY 10027, telephone (212) 854-4977, email roth@columbia.edu . SUPPLEMENTARY... York, NY 10027, telephone (212) 854-4977, email roth@columbia.edu , by August 29, 2013. After that...

  16. Evaluering af ny kokkeuddannelse for hørehandicappede

    DEFF Research Database (Denmark)

    Hansen, Niels-Henrik Møller

    2010-01-01

    Center for Ungdomsforskning har netop afsluttet en evaluering af en ny uddannelse indenfor madproduktion, rettet imod personer med et hørehandicap. Hensigten med uddannelsen er, at kunne tilbyde døve, døvblinde og hørehæmmede personer et uddannelsestilbud indenfor fremstilling af mad. Konkret han...

  17. 78 FR 44187 - New York Disaster # NY-00136

    Science.gov (United States)

    2013-07-23

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION New York Disaster NY-00136 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... locations. The following areas have been determined to be adversely affected by the disaster:...

  18. Evaluering af ny kokkeuddannelse for hørehandicappede

    DEFF Research Database (Denmark)

    Hansen, Niels-Henrik Møller

    2010-01-01

    Center for Ungdomsforskning har netop afsluttet en evaluering af en ny uddannelse indenfor madproduktion, rettet imod personer med et hørehandicap. Hensigten med uddannelsen er, at kunne tilbyde døve, døvblinde og hørehæmmede personer et uddannelsestilbud indenfor fremstilling af mad. Konkret han...

  19. Kapitalismens dna i ny oversættelse

    DEFF Research Database (Denmark)

    Schanz, Hans-Jørgen

    2013-01-01

    Adam Smiths klassiske storværk om kapitalismen ’Nationernes velstand’ havde stor indflydelse på socialfilosofien, på Hegel, på Marx og liberaløkonomerne. Ny oversættelse yder omsider værket fuld respekt på dansk. Anmeldelse af: Adam Smith: Nationernes velstand. 441+ 519 sider. 699,95 kr. Informat...

  20. 78 FR 7848 - New York Disaster Number NY-00130

    Science.gov (United States)

    2013-02-04

    ... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS ADMINISTRATION New York Disaster Number NY-00130 AGENCY: U.S. Small Business Administration. ACTION: Amendment 5.... Small Business Administration, Processing and Disbursement Center, 14925 Kingsport Road, Fort Worth,...

  1. Ny international efteruddannelse for fødevaresektoren

    DEFF Research Database (Denmark)

    Stacey, Julia; Brunsø, Karen

    2003-01-01

    Ny MBA på Handelshøjskolen i Århus stiller skarpt på fødevaresektoren. MBA - med fokus på fødevaresektoren henvender sig til personer med minimum tre års ledererfaring, der ønsker nye erhvervsrettede kompetencer på højt niveau....

  2. Kapitalismens dna i ny oversættelse

    DEFF Research Database (Denmark)

    Schanz, Hans-Jørgen

    2013-01-01

    Adam Smiths klassiske storværk om kapitalismen ’Nationernes velstand’ havde stor indflydelse på socialfilosofien, på Hegel, på Marx og liberaløkonomerne. Ny oversættelse yder omsider værket fuld respekt på dansk. Anmeldelse af: Adam Smith: Nationernes velstand. 441+ 519 sider. 699,95 kr. Informat...

  3. Interoperability of Demand Response Resources Demonstration in NY

    Energy Technology Data Exchange (ETDEWEB)

    Wellington, Andre

    2014-03-31

    The Interoperability of Demand Response Resources Demonstration in NY (Interoperability Project) was awarded to Con Edison in 2009. The objective of the project was to develop and demonstrate methodologies to enhance the ability of customer sited Demand Response resources to integrate more effectively with electric delivery companies and regional transmission organizations.

  4. 77 FR 50630 - Radio Broadcasting Services; Westfield, NY

    Science.gov (United States)

    2012-08-22

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Radio Broadcasting Services; Westfield, NY AGENCY: Federal Communications... Review Act, see 5 U.S.C. 801(a)(1)(A). List of Subjects in 47 CFR Part 73 Radio, Radio...

  5. NY-ESO-1 cancer testis antigen demonstrates high immunogenicity in triple negative breast cancer.

    Science.gov (United States)

    Ademuyiwa, Foluso O; Bshara, Wiam; Attwood, Kristopher; Morrison, Carl; Edge, Stephen B; Karpf, Adam R; James, Smith A; Ambrosone, Christine B; O'Connor, Tracey L; Levine, Ellis G; Miliotto, Anthony; Ritter, Erika; Ritter, Gerd; Gnjatic, Sacha; Odunsi, Kunle

    2012-01-01

    NY-ESO-1 cancer testis (CT) antigen is an attractive candidate for immunotherapy as a result of its high immunogenicity. The aim of this study was to explore the potential for NY-ESO-1 antigen directed immunotherapy in triple negative breast cancer (TNBC) by determining the frequency of expression by immunohistochemistry (IHC) and the degree of inherent immunogenicity to NY-ESO-1. 168 TNBC and 47 ER+/HER2- primary breast cancer specimens were used to determine NY-ESO-1 frequency by IHC. As previous studies have shown that patients with a robust innate humoral immune response to CT antigens are more likely to develop CD8 T-cell responses to NY-ESO-1 peptides, we evaluated the degree to which patients with NY-ESO-1 expression had inherent immunogenicity by measuring antibodies. The relationship between NY-ESO-1 expression and CD8+ T lymphocytes was also examined. The frequency of NY-ESO-1 expression in the TNBC cohort was 16% versus 2% in ER+/HER2- patients. A higher NY-ESO-1 score was associated with a younger age at diagnosis in the TNBC patients with NY-ESO-1 expression (p = 0.026). No differences in OS (p = 0.278) or PFS (p = 0.238) by NY-ESO-1 expression status were detected. Antibody responses to NY-ESO-1 were found in 73% of TNBC patients whose tumors were NY-ESO-1 positive. NY-ESO-1 positive patients had higher CD8 counts than negative patients (p = 0.018). NY-ESO-1 is expressed in a substantial subset of TNBC patients and leads to a high humoral immune response in a large proportion of these individuals. Given these observations, patients with TNBC may benefit from targeted therapies directed against NY-ESO-1.

  6. 33 CFR 207.60 - Federal Dam, Hudson River, Troy, N.Y.; pool level.

    Science.gov (United States)

    2010-07-01

    ..., N.Y.; pool level. 207.60 Section 207.60 Navigation and Navigable Waters CORPS OF ENGINEERS..., N.Y.; pool level. (a) Whenever the elevation of the pool created by the Federal dam at Troy, N.Y... automatically when the pool level rises to an elevation of +18.5 feet mean sea level, and conform in...

  7. 76 FR 20532 - Safety Zone; Boom Days, Niagara River, Niagara Falls, NY

    Science.gov (United States)

    2011-04-13

    ..., Niagara River, Niagara Falls, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the Niagara River, Niagara Falls, NY for the Boom... Niagara River, Niagara Falls, NY during the Boom Days Fireworks on April 16, 2011. This temporary...

  8. Elevation Certificates for Flood Prone Structures, Building Dept has required elevation certs when house falls inside flood zone - paper copies only, Published in 2010, 1:2400 (1in=200ft) scale, Effingham County Government.

    Data.gov (United States)

    NSGIC Local Govt | GIS Inventory — Elevation Certificates for Flood Prone Structures dataset current as of 2010. Building Dept has required elevation certs when house falls inside flood zone - paper...

  9. Geothermal energy and district heating in Ny-Ålesund, Svalbard

    OpenAIRE

    Iversen, Julianne

    2013-01-01

    This thesis presents the possibilities for using shallow geothermal energy for heating purposes in Ny-Ålesund. The current energy supply in Ny-Ålesund is a diesel generator, which does not comply with the Norwegian government and Ny-Ålesund Science Managers Committee’s common goal to maintain the natural environment in Ny-Ålesund. Ny-Ålesund has a potential for replacing the heat from the current diesel based energy source with geothermal energy. Geothermal energy is considered to have low im...

  10. Ny forskning: Derfor virker ros ikke mod stress

    DEFF Research Database (Denmark)

    Pedersen, Pernille

    2015-01-01

    Ny forskning peger på, at stressede medarbejdere overhører din ros og anerkendelse, hvis de føler skam. Skam over ikke at kunne slå til på arbejdet. Vil du hjælpe en stresset medarbejder, skal du forebygge, at de føler sig skamfulde. Læs her anbefalinger til, hvordan du bedst hjælper din stressed...

  11. Characterization of the near-Earth Asteroid 2002NY40

    OpenAIRE

    Roberts, Jr., Lewis C.; Hall, Doyle T.; Lambert, John V.; Africano, John L.; Knox, Keith T.; Barros, Jacob K.; Hamada, Kris M.; Liang, Dennis; Sydney, Paul F.; Kervin, Paul

    2007-01-01

    In August 2002, the near-Earth asteroid 2002 NY40, made its closest approach to the Earth. This provided an opportunity to study a near-Earth asteroid with a variety of instruments. Several of the telescopes at the Maui Space Surveillance System were trained at the asteroid and collected adaptive optics images, photometry and spectroscopy. Analysis of the imagery reveals the asteroid is triangular shaped with significant self-shadowing. The photometry reveals a 20-hour period and the spectros...

  12. Den fjerde vej: Trumps sejr skaber ny samfundsorden

    DEFF Research Database (Denmark)

    Rasmussen, Martin Møller Boje; Andersen, Torben K.; Kragh, Claus

    2016-01-01

    Bag Donald Trumps uempatiske retorik gemmer sig omridset af et politisk projekt, man kan kalde den fjerde vej. USA’s kommende præsident blander højre- og venstreorienterede mærkesager til et nyt nationalsocialt politisk koncept. Spørgsmålet er, om Trumps fjerde vej er et seriøst bud på en ny...

  13. NY-ESO-1 antibody as a novel tumour marker of gastric cancer.

    Science.gov (United States)

    Fujiwara, S; Wada, H; Kawada, J; Kawabata, R; Takahashi, T; Fujita, J; Hirao, T; Shibata, K; Makari, Y; Iijima, S; Nishikawa, H; Jungbluth, A A; Nakamura, Y; Kurokawa, Y; Yamasaki, M; Miyata, H; Nakajima, K; Takiguchi, S; Nakayama, E; Mori, M; Doki, Y

    2013-03-19

    NY-ESO-1 antibodies are specifically observed in patients with NY-ESO-1-expressing tumours. We analysed whether the NY-ESO-1 humoral immune response is a useful tumour marker of gastric cancer. Sera from 363 gastric cancer patients were screened by enzyme-linked immunosorbent assay (ELISA) to detect NY-ESO-1 antibodies. Serial serum samples were obtained from 25 NY-ESO-1 antibody-positive patients, including 16 patients with curative resection and 9 patients who received chemotherapy alone. NY-ESO-1 antibodies were detected in 3.4% of stage I, 4.4% of stage II, 25.3% of stage III, and 20.0% of stage IV patients. The frequency of antibody positivity increased with disease progression. When the NY-ESO-1 antibody was used in combination with carcinoembryonic antigen and CA19-9 to detect gastric cancer, information gains of 11.2% in stages III and IV, and 5.8% in all patients were observed. The NY-ESO-1 immune response levels of the patients without recurrence fell below the cutoff level after surgery. Two of the patients with recurrence displayed incomplete decreases. The nine patients who received chemotherapy alone continued to display NY-ESO-1 immune responses. When combined with conventional tumour markers, the NY-ESO-1 humoral immune response could be a useful tumour marker for detecting advanced gastric cancer and inferring the post-treatment tumour load in seropositive patients.

  14. NY-ESO-1 expression and immunogenicity in prostate cancer patients.

    Science.gov (United States)

    Gati, Asma; Lajmi, Nesrine; Derouiche, Amine; Marrakchi, Raja; Chebil, Mohamed; Benammar-Elgaaied, Amel

    2011-10-01

    Prostate cancer is the second leading cause of men cancer-related death. Cancer immunotherapy has been investigated as a treatment which might be instituted at the point of detection of androgen-independent metastatic disease. to investigate the expression and humoral response against NYESO-1 in patients with prostate cancer (PC) and to analyze the relationship between expression of NY-ESO-1 and clinicopathological features. NY-ESO-1 mRNA in surgically resected PC and benign prostatic hyperplasia (BPH) were examined by reverse transcriptionpolymerase chain reaction. The antibody response to NY-ESO-1 was examined by enzyme-linked Elisa assay using recombinant NYESO-1 protein. NY-ESO-1 mRNA was detected in 9 of 23 (39%) PC patients. Antibodies against NY-ESO-1 protein were detected in 12 of 23 (52%) sera of PC patients and in 5 of 9 (55%) of NY-ESO-1 expressing tumors. However, no mRNA copy or NY-ESO-1 antibodies were detected in all BPH patients tested. The present study has demonstrated the expression of NY-ESO-1mRNA in prostate Cancer patients and NY-ESO-1 antibody production. Our data suggest that NY-ESO-1 could be used as a tumor marker and constitute a good candidate for vaccine-based immunotherapy for hormonal resistant prostate cancer patients.

  15. Vaccination with NY-ESO-1 overlapping peptides mixed with Picibanil OK-432 and montanide ISA-51 in patients with cancers expressing the NY-ESO-1 antigen.

    Science.gov (United States)

    Wada, Hisashi; Isobe, Midori; Kakimi, Kazuhiro; Mizote, Yu; Eikawa, Shingo; Sato, Eiichi; Takigawa, Nagio; Kiura, Katsuyuki; Tsuji, Kazuhide; Iwatsuki, Keiji; Yamasaki, Makoto; Miyata, Hiroshi; Matsushita, Hirokazu; Udono, Heiichiro; Seto, Yasuyuki; Yamada, Kazuhiro; Nishikawa, Hiroyoshi; Pan, Linda; Venhaus, Ralph; Oka, Mikio; Doki, Yuichiro; Nakayama, Eiichi

    2014-01-01

    We conducted a clinical trial of an NY-ESO-1 cancer vaccine using 4 synthetic overlapping long peptides (OLP; peptides #1, 79-108; #2, 100-129; #3, 121-150; and #4, 142-173) that include a highly immunogenic region of the NY-ESO-1 molecule. Nine patients were immunized with 0.25 mg each of three 30-mer and a 32-mer long NY-ESO-1 OLP mixed with 0.2 KE Picibanil OK-432 and 1.25 mL Montanide ISA-51. The primary endpoints of this study were safety and NY-ESO-1 immune responses. Five to 18 injections of the NY-ESO-1 OLP vaccine were well tolerated. Vaccine-related adverse events observed were fever and injection site reaction (grade 1 and 2). Two patients showed stable disease after vaccination. An NY-ESO-1-specific humoral immune response was observed in all patients and an antibody against peptide #3 (121-150) was detected firstly and strongly after vaccination. NY-ESO-1 CD4 and CD8 T-cell responses were elicited in these patients and their epitopes were identified. Using a multifunctional cytokine assay, the number of single or double cytokine-producing cells was increased in NY-ESO-1-specific CD4 and CD8 T cells after vaccination. Multiple cytokine-producing cells were observed in PD-1 (-) and PD-1 (+) CD4 T cells. In conclusion, our study indicated that the NY-ESO-1 OLP vaccine mixed with Picibanil OK-432 and Montanide ISA-51 was well tolerated and elicited NY-ESO-1-specific humoral and CD4 and CD8 T-cell responses in immunized patients.

  16. 33 CFR 110.87 - Henderson Harbor, N.Y.

    Science.gov (United States)

    2010-07-01

    ... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.87 Henderson Harbor, N.Y. (a) Area A. The area in the... latitude 43°51′08.8″ N, longitude 76°12′08.9″ W, thence to latitude 43°51′09.0″ N, longitude 76°12′19.0″ W, thence to latitude 43°51′33.4″ N, longitude 76°12′19.0″ W, thence to latitude 43°51′33.4″ N, longitude...

  17. NY-ESO-1 — EDRN Public Portal

    Science.gov (United States)

    NY-ESO-1, official name CTAG1B, is an antigen that is expressed in normal testis and ovary and overexpressed in a wide variety of cancers. The CTAG1B gene is found in a duplicated region of the X-chromosome and therefore has a neighboring gene of identical sequence. CTAG1B is expressed from 18 weeks until birth in human fetal testis. In the adult testis, is strongly expressed in spermatogonia and in primary spermatocytes, but not in post-meiotic cells or in testicular somatic cells (at protein level).

  18. Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma

    OpenAIRE

    Mara Giavina-Bianchi; Pedro Giavina-Bianchi; Mirian Nacagami Sotto; Alona Muzikansky; Jorge Kalil; Cyro Festa-Neto; Duncan, Lyn M.

    2015-01-01

    NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79), rarely in in situ melanoma (1/10) and not in benign nevi (0/20). Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. N...

  19. Increased NY-ESO-1 Expression and Reduced Infiltrating CD3+ T Cells in Cutaneous Melanoma

    Directory of Open Access Journals (Sweden)

    Mara Giavina-Bianchi

    2015-01-01

    Full Text Available NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79, rarely in in situ melanoma (1/10 and not in benign nevi (0/20. Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. NY-ESO-1 expression was associated with increased primary tumor thickness (P=0.007 and inversely correlated with superficial spreading melanoma (P<0.02. NY-ESO-1 expression was also associated with reduced numbers and density of CD3+ tumor infiltrating lymphocytes (P=0.017. When NY-ESO-1 protein was expressed, CD3+ T cells were less diffusely infiltrating the tumor and were more often arranged in small clusters (P=0.010 or as isolated cells (P=0.002 than in large clusters of more than five lymphocytes. No correlation of NY-ESO-1 expression with gender, age, tumor site, ulceration, lymph node sentinel status, or survival was observed. NY-ESO-1 expression in melanoma was associated with tumor progression, including increased tumor thickness, and with reduced tumor infiltrating lymphocytes.

  20. NY-ESO-1 protein glycosylated by yeast induces enhanced immune responses.

    Science.gov (United States)

    Wadle, Andreas; Mischo, Axel; Strahl, Sabine; Nishikawa, Hiroyoshi; Held, Gerhard; Neumann, Frank; Wullner, Beate; Fischer, Eliane; Kleber, Sascha; Karbach, Julia; Jager, Elke; Shiku, Hiroshi; Odunsi, Kunle; Shrikant, Protul A; Knuth, Alexander; Cerundolo, Vincenzo; Renner, Christoph

    2010-11-01

    Vaccine strategies that target dendritic cells to elicit potent cellular immunity are the subject of intense research. Here we report that the genetically engineered yeast Saccharomyces cerevisiae, expressing the full-length tumour-associated antigen NY-ESO-1, is a versatile host for protein production. Exposing dendritic cells (DCs) to soluble NY-ESO-1 protein linked to the yeast a-agglutinin 2 protein (Aga2p) protein resulted in protein uptake, processing and MHC class I cross-presentation of NY-ESO-1-derived peptides. The process of antigen uptake and cross-presentation was dependent on the glycosylation pattern of NY-ESO-1-Aga2p protein and the presence of accessible mannose receptors. In addition, NY-ESO-1-Aga2p protein uptake by dendritic cells resulted in recognition by HLA-DP4 NY-ESO-1-specific CD4(+) T cells, indicating MHC class II presentation. Finally, vaccination of mice with yeast-derived NY-ESO-1-Aga2p protein led to an enhanced humoral and cellular immune response, when compared to the bacterially expressed NY-ESO-1 protein. Together, these data demonstrate that yeast-derived full-length NY-ESO-1-Aga2p protein is processed and presented efficiently by MHC class I and II complexes and warrants clinical trials to determine the potential value of S. cerevisiae as a host for cancer vaccine development. Copyright © 2010 John Wiley & Sons, Ltd.

  1. NY-ESO-1 expression in synovial sarcoma and other mesenchymal tumors: significance for NY-ESO-1-based targeted therapy and differential diagnosis.

    Science.gov (United States)

    Lai, Jin-Ping; Robbins, Paul F; Raffeld, Mark; Aung, Phyu Phyu; Tsokos, Maria; Rosenberg, Steven A; Miettinen, Markku M; Lee, Chyi-Chia Richard

    2012-06-01

    A promising targeted therapy against NY-ESO-1 (CTAG 1B) using genetically modified T-cells in synovial sarcomas was recently demonstrated in a clinical trial at the NCI. To investigate the role of NY-ESO-1 immunohistochemistry in patient selection and gain better insight into the incidence of NY-ESO-1 expression in synovial sarcomas and other mesenchymal tumors, we evaluated NY-ESO-1 expression by immunohistochemistry in 417 tumors. This collection of samples included: 50 SS18/SSX1/2 fusion positive synovial sarcomas, 155 gastrointestinal stromal tumors (GIST), 135 other spindle cell sarcomas as well as 77 other sarcomas (chondrosarcoma, osteosarcoma, dedifferentiated liposarcoma, alveolar soft part sarcoma, rhabdomyosarcoma, angiosarcoma, malignant mesothelioma, and Ewing's sarcoma). We report that 76% of synovial sarcomas expressed NY-ESO-1 in a strong and diffuse pattern (2-3+, >50-70% of tumor cells). In contrast, only rare cases of other spindle cell mesenchymal tumor expressed NY-ESO-1 (GIST (2/155), malignant peripheral nerve sheath tumors (1/34), and dermatofibrosarcoma protuberans (2/20)). Individual cases of other sarcomas (angiosarcoma, malignant mesothelioma, chondrosarcoma, osteosarcoma, dedifferentiated liposarcoma, alveolar soft part sarcoma, and Ewing's sarcoma) were positive for NY-ESO-1. However, no positive cases were identified amongst our cohort of leiomyosarcomas (0/24), hemangiopericytoma/solitary fibrous tumors (0/40), and cellular schwannomas (0/17). In summary, we find that NY-ESO-1 is strongly and diffusely expressed in a majority of synovial sarcomas, but only rarely in other mesenchymal lesions. Beyond its role in patient selection for targeted therapy, immunohistochemistry for NY-ESO-1 may be diagnostically useful for the distinction of synovial sarcoma from other spindle cell neoplasms.

  2. Atmospheric Research in Ny-Aalesund. Proceedings from the Third NySMAC meeting. NILU, Kjeller, Norway, 9.-11. April 1997

    Energy Technology Data Exchange (ETDEWEB)

    Floeisand, Inga; Gernandt, Hartwig; Larsen, Elisabeth Stoltz; Stordal, Frode; Wada, Makoto

    1997-07-01

    Leading abstract. The Ny-Aalesund Seminar Series was conceived by NySMAC (Ny-Aalesund Science Managers Committee) as a means of bringing together research scientists involved in programmes running at Ny-Aalesund or having an interest in that area. The first Seminar was held in Potsdam in Germany in May 1995, and included talks which covered all current research disciplines; physical, atmospheric, earth and biological sciences. The Second Seminar was devoted to biological science and particularly to ecology. It was held in Cambridge in UK in February 1996. The subject of this Third Seminar, held in Kjeller in Norway in April 1997, is atmospheric research in Ny-Aalesund. Since the early 1980s a wide range of projects have been carried out on Svalbard and particularly at Ny-Aalesund. Prominent in this research have been several international research programmes with emphasis on topics of e.g. Arctic (Arctic Monitoring and Assessment Programme, AMAP), European (European Monitoring and Evaluation Programme, EMEP) or global (International Geosphere-Biosphere Programme, IGBP; World Climate Research Programme, WCRP) scales. Atmospheric observations have been carried out in Ny-Aalesund in Svalbard for several years. From the early 1980s, the long range transport of sulphur dioxide and sulphate aerosols leading to Arctic Haze, has been studied. As a consequence, EMEP included Ny-Aalesund in their network. In the following years the transport of ozone and chemical precursors were observed, and recently the naturally occurring phenomenon of rapid surface ozone loss has been given particular attention. From the late 1980s observations of the stratospheric ozone layer and related chemical species were undertaken, and the efforts have increased considerably since. Ny-Aalesund is now one of the primary Arctic sites of the NDSC (Network for Detection of Stratospheric Change) network. Important efforts have been undertaken to participate in the European campaigns as the European

  3. NY-ESO-1 expression predicts an aggressive phenotype of ovarian cancer.

    Science.gov (United States)

    Szender, J Brian; Papanicolau-Sengos, Antonios; Eng, Kevin H; Miliotto, Anthony J; Lugade, Amit A; Gnjatic, Sacha; Matsuzaki, Junko; Morrison, Carl D; Odunsi, Kunle

    2017-06-01

    NY-ESO-1 is a cancer testis antigen and a promising target for immunotherapy. The purpose of this study was to determine the expression frequency, immunogenicity, and clinical impact of NY-ESO-1 in ovarian cancer. Immunohistochemistry (IHC), reverse-transcription polymerase chain reaction (RT-PCR), and quantitative-PCR (qRT-PCR) were utilized in an ovarian cancer (including Fallopian tube and primary peritoneal cancers) patient cohort; humoral responses against NY-ESO-1 were determined by ELISA. Clinicopathologic outcomes including progression-free (PFS) and overall (OS) survival were evaluated based on NY-ESO-1 expression. Cohen's kappa (κ) tested agreement between expression tests. NY-ESO-1 expression was detected by any method in 40.7% of 1002 patients' tumors (NY-ESO-1+) and baseline humoral response was identified in 19.0% of 689 tested patients. NY-ESO-1+ patients were older (pESO-1+ patients. A subset analysis of NY-ESO-1+ patients that received immunotherapy demonstrated improved OS by >2years (52.6 vs. 27.2months, pESO-1 expression and an aggressive cancer phenotype. The relatively high expression frequency of NY-ESO-1 in ovarian cancer patients coupled with the poor clinical outcomes in NY-ESO-1+ patients reveals an underappreciated need for targeted therapy against this antigen. In support, our study reveals that NY-ESO-1+ patients enrolled on immunotherapy trials targeting the antigen exhibited an improvement in OS. Copyright © 2017 Elsevier Inc. All rights reserved.

  4. Archive of Datasonics SIS-1000 Chirp Subbottom Data Collected During USGS Cruise DIAN 96040 Long Island, NY Inner Shelf -- Fire Island, NY, 4-24 September, 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 96040 cruise. The coverage is the nearshore of Long Island, NY in...

  5. Archive of Datasonics SIS-1000 Chirp Subbottom Data Collected During USGS Cruise DIAN 96040 Long Island, NY Inner Shelf -- Fire Island, NY, 4-24 September, 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 96040 cruise. The coverage is the nearshore of Long Island, NY in...

  6. NOAA Office for Coastal Management Benthic Habitat Data, Long Island Sound, Jamaica Bay, and Lower Bay of NY/NJ Harbor, NY, 1994-2002 (NODC Accession 0089467)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data are a collection of benthic habitat data from studies conducted in the coastal Long Island Sound, NY region in GIS shapefile (.shp, .dbf, .shx, and .prj...

  7. NY-ESO-1抗原与肿瘤免疫治疗进展%NY-ESO-1 and Cancer Immunotherapy

    Institute of Scientific and Technical Information of China (English)

    彭吉润; 冷希圣

    2008-01-01

    NY-ESO-1抗原是肿瘤-睾丸抗原家族中的重要成员,在多种类型的肿瘤中广泛表达,并且是迄今发现的最具免疫原性的肿瘤抗原,可以在NY-ESO-1表达阳性的肿瘤患者体内引起自发性体液免疫反应和特异性T细胞免疫反应.因此,NY-ESO-1被认为是最好的肿瘤候选疫苗,在肿瘤免疫治疗中最具发展潜力.本文主要介绍了近年来有关NY-ESO-1抗原以及相关疫苗的研究进展.

  8. Progress in NY-ESO-I as cancer vaccines%NY-ESO-1癌症疫苗研究进展

    Institute of Scientific and Technical Information of China (English)

    刘志青

    2006-01-01

    NY-ESO-1抗原是近年来发现的一种肿瘤特异性抗原,在乳腺癌、黑色素瘤、食管癌等20多种肿瘤中均有不同程度的表达.NY-ESO-1的广泛表达使其成为多种实体瘤特异性疫苗免疫治疗的首选抗原.在制备NY-ESO-1癌症疫苗时,目前主要围绕癌症疫苗的类型、抗原的加工提呈两个方面进行研究.现综述近年来NY-ESO-1癌症疫苗在这两方面的研究进展.

  9. Archive of Datasonics SIS-1000 Chirp Subbottom Data Collected During USGS Cruise DIAN 96040 Long Island, NY Inner Shelf -- Fire Island, NY, 4-24 September, 1996

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 96040 cruise. The coverage is the nearshore of Long Island, NY...

  10. Induction of immune response against NY-ESO-1 by CHP-NY-ESO-1 vaccination and immune regulation in a melanoma patient.

    Science.gov (United States)

    Tsuji, Kazuhide; Hamada, Toshitada; Uenaka, Akiko; Wada, Hisashi; Sato, Eiichi; Isobe, Midori; Asagoe, Kenji; Yamasaki, Osamu; Shiku, Hiroshi; Ritter, Gerd; Murphy, Roger; Hoffman, Eric W; Old, Lloyd J; Nakayama, Eiichi; Iwatsuki, Keiji

    2008-10-01

    NY-ESO-1 is a cancer/testis antigen highly immunogenic in cancer patients. Cholesterol-bearing hydrophobized pullulan (CHP) is a nanoparticle-forming antigen-delivery vehicle and CHP complexed with NY-ESO-1 protein (CHP-NY-ESO-1) efficiently activates CD4 and CD8 T cells in vitro. In this study we report on a 50-year-old male melanoma patient with multiple skin and organ metastases (T4N3M1c) who was vaccinated with CHP-NY-ESO-1 at biweekly intervals and who had an unusual disease course. We characterized in this patient humoral and cellular immune responses, immune regulatory cells, and cytokine profiles in the peripheral blood and at local tumor sites. Ten days after the second CHP-NY-ESO-1 vaccination (day 25), blisters appeared on the skin at the metastatic lesions associated with inflammatory changes. A skin biopsy showed the presence of many NY-ESO-1-expressing apoptotic melanoma cells as determined by a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) test. However, the tumors continued to grow, and the patient died of pulmonary failure due to multiple metastases on day 48. Serum antibody responses were detected after the second CHP-NY-ESO-1 vaccination and antibody titer increased with subsequent vaccinations. Th1 dependent IgG1 was the predominant immunoglobulin subtype. Both, NY-ESO-1-specific CD4 and CD8 T cell responses were detected in PBMC by IFN-gamma secretion assays. After CHP-NY-ESO-1 vaccination a slight decrease in CD4(+)CD25(+)Foxp3(+) Tregs was observed in PBMC but significantly increased numbers of CD4(+)CD25(+)Foxp3(+) Tregs and CD68(+) immunoregulatory macrophages were detected at the local tumor sites. CD4(+)CD25(+)Foxp3(+) Tregs were also increased in the blister fluid. Cytokines in the serum suggested a polarization towards a Th1 pattern in the PBMC and those in the blister fluid suggested a Th2-type response at the tumor site. Our observations indicate induction of specific humoral and

  11. Wind-blown sand movement periods on the Nyírség alluvial fan, Hungary

    Science.gov (United States)

    Buró, Botond; Lóki, József; Sipos, György; Négyesi, Gábor; Andrási, Bence; Jakab, Attila; Félegyházi, Enikő; Molnár, Mihály

    2017-04-01

    Wind-blown sand movement periods on the Nyírség alluvial fan, Hungary The Nyírség is an alluvial fan, was built by rivers, which were flown down from the NE Carpathians. When the weather was dry, wind-blown sand was blowed out and the wind started to develop the aeolian landforms. The first significant sand movements in the Nyírség was in the Upper plenniglacial and the Late glacial. The main landforms of the Nyírség were developed at this time. The aeolic transformation of the land was not completed in the Nyírség at the end of the Pleistocene. In the Holocene the sand moved within small area, mainly by anthropogenic impact. Our aim is clarify the age of the wind-blown sand movements period, with different absolute (Radiocarbon dating method, OSL), and relative (Archaeological finds, Pollen analyses) dating methods. We have collected for the age determining charcoal from many sand quarry (Gégény, Kántorjánosi, Nyíradony, Nagyvarsány, Máriapócs and Lövőpetri) which contain fossil soil layer. For the OSL measurements samples were collected from Baktalórántháza, Gégény and Kántorjánosi sand quarries. We collected samples from 5 places for the pollen analyses (Nyírtanya, Máriapócs, Nyírbéltek-Nyírlúgos, Nyírábrány, Vámospércs), and also from an archaeological excavation (Nyíregyháza- Oros). The new age dates show us, that in the Nyírség the first significant sand movements were in the cold and drier period of the Upper pleniglacial and Late glacial (Baktalórántháza, Nyírtanya, Vámospércs). At the end of the Pleistocene the sand movements not competed in the Nyírség (Gégény, Kántorjánosi, Nyírábrány). In the Holocene there were many little sand movement periods, mainly caused by anthropogenic impact (Nyíradony, Nyíregyháza-Oros). The research was supported by the European Union and the State of Hungary, co-financed by the European Regional Development Fund in the project of GINOP-2.3.2.-15-2016-00009 'ICER

  12. 33 CFR 110.150 - Block Island Sound, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Block Island Sound, N.Y. 110.150 Section 110.150 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.150 Block Island Sound, N.Y. (a) The anchorage ground. A...

  13. 33 CFR 110.85 - Niagara River, Youngstown, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Niagara River, Youngstown, N.Y. 110.85 Section 110.85 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.85 Niagara River, Youngstown, N.Y. (a) Area...

  14. 78 FR 35596 - Special Local Regulation; Long Beach Regatta, Powerboat Race, Atlantic Ocean, Long Beach, NY

    Science.gov (United States)

    2013-06-13

    ... SECURITY Coast Guard 33 CFR Part 100 RIN 1625-AA08 Special Local Regulation; Long Beach Regatta, Powerboat Race, Atlantic Ocean, Long Beach, NY AGENCY: Coast Guard, DHS. ACTION: Notice of Proposed Rulemaking... Atlantic Ocean off Long Beach, NY during the Long Beach Regatta Powerboat Race scheduled for August...

  15. Epigenetic potentiation of NY-ESO-1 vaccine therapy in human ovarian cancer.

    Science.gov (United States)

    Odunsi, Kunle; Matsuzaki, Junko; James, Smitha R; Mhawech-Fauceglia, Paulette; Tsuji, Takemasa; Miller, Austin; Zhang, Wa; Akers, Stacey N; Griffiths, Elizabeth A; Miliotto, Anthony; Beck, Amy; Batt, Carl A; Ritter, Gerd; Lele, Shashikant; Gnjatic, Sacha; Karpf, Adam R

    2014-01-01

    The cancer-testis/cancer-germline antigen NY-ESO-1 is a vaccine target in epithelial ovarian cancer (EOC), but its limited expression is a barrier to vaccine efficacy. As NY-ESO-1 is regulated by DNA methylation, we hypothesized that DNA methyltransferase (DNMT) inhibitors may augment NY-ESO-1 vaccine therapy. In agreement, global DNA hypomethylation in EOC was associated with the presence of circulating antibodies to NY-ESO-1. Pre-clinical studies using EOC cell lines showed that decitabine treatment enhanced both NY-ESO-1 expression and NY-ESO-1-specific CTL-mediated responses. Based on these observations, we performed a phase I dose-escalation trial of decitabine, as an addition to NY-ESO-1 vaccine and doxorubicin liposome (doxorubicin) chemotherapy, in 12 patients with relapsed EOC. The regimen was safe, with limited and clinically manageable toxicities. Both global and promoter-specific DNA hypomethylation occurred in blood and circulating DNAs, the latter of which may reflect tumor cell responses. Increased NY-ESO-1 serum antibodies and T cell responses were observed in the majority of patients, and antibody spreading to additional tumor antigens was also observed. Finally, disease stabilization or partial clinical response occurred in 6/10 evaluable patients. Based on these encouraging results, evaluation of similar combinatorial chemo-immunotherapy regimens in EOC and other tumor types is warranted.

  16. Expression and immunogenicity of NY-ESO-1 in colorectal cancer.

    Science.gov (United States)

    Li, Ya; Song, Ruifeng; Li, Xinqiang; Xu, Feng

    2017-06-01

    Serum assays of NY-ESO-1 antibodies provide a guide to discriminate between patients who suffer from different types of cancer. In the present study, the expression of NY-ESO-1 was detected with the aim to identify a novel tumor antigen in colorectal cancer (CRC). Sera were obtained from 89 healthy individuals and 236 patients with CRC with stage I, II, III and IV tumors. The NY-ESO-1 autoantibody levels were determined using an enzyme-linked immunosorbent assay. The mRNA and protein expression levels of NY-ESO-1 were detected using reverse transcription-polymerase chain reaction and immunohistochemistry, respectively, in 60 CRC and paired adjacent non-tumor tissues. NY-ESO-1 antibody was detected in 40 of the 236 (16.9%) patients with CRC. The NY-ESO-1 antibody combined with carcinoembryonic antigen enhanced the sensitivity, from 52.1 to 62.7%, of the diagnosis of CRC. The frequency of antibody positivity increased with the TNM cancer stage (8.8 vs. 28.3% in stages I+II and III+IV, respectively). The mRNA and protein expression levels of NY-ESO-1 were significantly higher in CRC tissue than in adjacent non-tumor tissue. In conclusion, NY-ESO-1 is frequently expressed in CRC with the capacity of inducing a humoral immune response in CRC patients, exhibiting the potential to be a promising biomarker for CRC.

  17. Increased NY-ESO-1 expression and reduced infiltrating CD3+ T cells in cutaneous melanoma.

    Science.gov (United States)

    Giavina-Bianchi, Mara; Giavina-Bianchi, Pedro; Sotto, Mirian Nacagami; Muzikansky, Alona; Kalil, Jorge; Festa-Neto, Cyro; Duncan, Lyn M

    2015-01-01

    NY-ESO-1 is a cancer-testis antigen aberrantly expressed in melanomas, which may serve as a robust and specific target in immunotherapy. NY-ESO-1 antigen expression, tumor features, and the immune profile of tumor infiltrating lymphocytes were assessed in primary cutaneous melanoma. NY-ESO-1 protein was detected in 20% of invasive melanomas (16/79), rarely in in situ melanoma (1/10) and not in benign nevi (0/20). Marked intratumoral heterogeneity of NY-ESO-1 protein expression was observed. NY-ESO-1 expression was associated with increased primary tumor thickness (P = 0.007) and inversely correlated with superficial spreading melanoma (P ESO-1 expression was also associated with reduced numbers and density of CD3+ tumor infiltrating lymphocytes (P = 0.017). When NY-ESO-1 protein was expressed, CD3+ T cells were less diffusely infiltrating the tumor and were more often arranged in small clusters (P = 0.010) or as isolated cells (P = 0.002) than in large clusters of more than five lymphocytes. No correlation of NY-ESO-1 expression with gender, age, tumor site, ulceration, lymph node sentinel status, or survival was observed. NY-ESO-1 expression in melanoma was associated with tumor progression, including increased tumor thickness, and with reduced tumor infiltrating lymphocytes.

  18. NY-ESO-1-specific immunological pressure and escape in a patient with metastatic melanoma.

    Science.gov (United States)

    von Boehmer, Lotta; Mattle, Muriel; Bode, Peter; Landshammer, Alexandro; Schäfer, Carolin; Nuber, Natko; Ritter, Gerd; Old, Lloyd; Moch, Holger; Schäfer, Niklaus; Jäger, Elke; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    During cancer progression, malignant cells may evade immunosurveillance. However, evidence for immunological escape in humans is scarce. We report here the clinical course of a melanoma patient whose initial tumor was positive for the antigens NY-ESO-1, MAGE-C1, and Melan-A. Upon immunization with a recombinant vaccinia/fowlpox NY-ESO-1 construct, the patient experienced a mixed clinical response and spreading of the NY-ESO-1 epitopes in the CD4+ T cell compartment. After NY-ESO-1 protein + CpG immunization, the patient's anti-NY-ESO-1 IgG response increased. Over the following years, progressing lesions were resected and found to be NY-ESO-1-negative while being positive for MAGE-C1, Melan-A, and MHC-I. The fatal, inoperable brain metastasis was analyzed after his death and also proved to be NY-ESO-1-negative, while being positive for MAGE-C1 and Melan-A, as well as MHC-I. We propose that cancer control and cancer escape in this patient were governed by NY-ESO-1-specific immunological pressure. Our findings provide evidence for the existence of immunoediting and immunoescape in this cancer patient.

  19. 75 FR 38710 - Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY

    Science.gov (United States)

    2010-07-06

    ... Championships, Huntington Bay, NY'' in the Federal Register (75 FR 13454). The Coast Guard received no comments...-2009-0520) in the Federal Register (74 FR 51243). The notice proposed a regulated area encompassing 100... Bay, NY in the Federal Register (75 FR 13454). This notice proposes a 100 yard regulated area...

  20. 77 FR 35113 - Canadian National Railway Company-Abandonment Exemption-in Niagara County, NY

    Science.gov (United States)

    2012-06-12

    ... milepost 0.20 to approximately milepost 0.35 in the City of Niagara Falls, Niagara County, N.Y., a distance... track in Niagara Falls, extending between the eastern end of the Bridge and the beginning of the Niagara... Niagara Falls, N.Y., and Niagara Falls, Ontario, Canada, and is owned by the bi-national Niagara......

  1. 33 CFR 165.170 - Safety Zone: Triathlon, Ulster Landing, Hudson River, NY.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Safety Zone: Triathlon, Ulster Landing, Hudson River, NY. 165.170 Section 165.170 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.170 Safety Zone: Triathlon, Ulster Landing, Hudson River, NY. (a) Regulated area. The...

  2. 77 FR 30451 - Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY

    Science.gov (United States)

    2012-05-23

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY... establish a temporary safety zone on Lake Ontario, Olcott, NY. This proposed rule is intended to restrict vessels from a portion of Lake Ontario during the Olcott fireworks display. The safety zone established...

  3. Bacterial Diversity of Ny-Ålesund, Arctic Archipelago Svalbard

    Directory of Open Access Journals (Sweden)

    Battsetseg Choidash

    2012-12-01

    Full Text Available The bacterial diversity of the water sample, collected from Ny-Ålesund, Arctic Archipelago Svalbard was analyzed by a phenotypic as well as a genotypic approach. Pure colonies of the culturable bacteria were established and grown at a range of temperatures: 4ºC, 15ºC, 22ºC and 37ºC. Optimum growth was found at 15ºC, and around 28 colonies were obtained. The library was dominated by 16S rDNAs of Gram-negative bacteria ( γ -Proteobacteria. Twenty two isolates exhibited sequences were similar to that of known bacterial isolates (>97% sequence similarity, represented by the species of the genera Psychrobacter , Pseudomonas , and Acinetobacter . Six isolates exhibited sequences showed less affi liation with known taxa (<97% sequence similarity, and may represent novel taxa.

  4. T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.

    Science.gov (United States)

    Singh, Nathan; Kulikovskaya, Irina; Barrett, David M; Binder-Scholl, Gwendolyn; Jakobsen, Bent; Martinez, Daniel; Pawel, Bruce; June, Carl H; Kalos, Michael D; Grupp, Stephan A

    The cancer-testis antigen NY-ESO-1 is expressed by many solid tumors and has limited expression by mature somatic tissues, making it a highly attractive target for tumor immunotherapy. Targeting NY-ESO-1 using engineered T cells has demonstrated clinical efficacy in the treatment of some adult tumors. Neuroblastoma is a significant cause of cancer mortality in children, and is a tumor type shown to be responsive to immunotherapies. We evaluated a large panel of primarily resected neuroblastoma samples and demonstrated that 23% express NY-ESO-1. After confirming antigen-specific activity of T cells genetically engineered to express an NY-ESO-1 directed high-affinity transgenic T cell receptor in vitro, we performed xenograft mouse studies assessing the efficacy of NY-ESO-1-targeted T cells in both localized and disseminated models of neuroblastoma. Disease responses were monitored by tumor volume measurement and in vivo bioluminescence. After delivery of NY-ESO-1 transgenic TCR T cells, we observed significant delay of tumor progression in mice bearing localized and disseminated neuroblastoma, as well as enhanced animal survival. These data demonstrate that NY-ESO-1 is an antigen target in neuroblastoma and that targeted T cells represent a potential therapeutic option for patients with neuroblastoma.

  5. Expression of MAGE-A and NY-ESO-1 in Primary and Metastatic Cancers.

    Science.gov (United States)

    Park, Tristen S; Groh, Eric M; Patel, Krishna; Kerkar, Sid P; Lee, Chyi-Chia Richard; Rosenberg, Steven A

    2016-01-01

    Melanoma-associated antigen-A (MAGE-A) and New York esophageal squamous cell cancer-1 (NY-ESO-1) are 2 cancer testis antigens (CTA) demonstrating potential for use in targeted immunotherapy. Clinical trials in melanoma and synovial sarcomas targeting these antigens in immune-based therapies have demonstrated durable tumor regression. Although protein expression of NY-ESO-1 has been assessed in a variety of cancer types, the expression of MAGE-A has not been studied in depth. In this study we analyzed MAGE-A and NY-ESO-1 expression in 314 melanoma specimens from 301 melanoma patients, 38 patients with squamous cell cancers and 111 patients with adenocarcinomas. Our results demonstrated higher expression of MAGE-A compared with NY-ESO-1 in melanomas (32% vs. 13%) and squamous cell carcinomas (45% vs. 7.9%), and higher expression of both CTAs in metastatic versus primary tumors. CTA expression in adenocarcinomas was low (MAGE-A: 10%, NY-ESO-1: 0.9%). In addition, we looked at concordance of expression among metastatic melanoma lesions within the same patient and found concordant expression in 38 of 47 patients for MAGE-A and 43 of 47 patients for NY-ESO-1. Our study demonstrated that the MAGE-A family may be of greater utility than NY-ESO-1 for targeted immunotherapy in a variety of cancer histologies, in particular metastatic melanomas and squamous cell carcinomas.

  6. Expression of NY-ESO-1 ,NY-SAR-35 in retinoblastoma and its clinical significance%视网膜母细胞瘤中NY-ESO-1、NY-SAR-35基因的表达及其临床意义

    Institute of Scientific and Technical Information of China (English)

    严宇清; 林泉; 孔旻; 何剑峰; 陈迎迎; 谢小薰; 罗彬; 梁皓

    2012-01-01

    背景 视网膜母细胞瘤(RB)的免疫治疗因创伤小、针对性强而受到广泛关注,寻求相关免疫原性较强的肿瘤抗原是免疫治疗的基础.NY-ESO-I和NY-SAR-35是癌-睾丸抗原(CTA)中的两种基因,检测其在RB组织中的表达可为RB的免疫治疗研究提供依据. 目的 研究RB中NY-ESO-1 mRNA、NY-SAR-35 mRNA的表达情况,探讨将其用于RB特异性免疫治疗靶抗原的可能性. 方法 收集15例RB患儿组织为RB组、12例非肿瘤病变部位视网膜组织为非肿瘤病变组,及22例眼部因其他眼部病变进行手术治疗获取的正常相关眼组织标本为正常组.采用逆转录聚合酶链反应(RT-PCR)检测待测组织标本中NY-ESO-1mRNA、NY-SAR-35 mRNA的表达;并按随机数字表法抽取RT-PCR检测的阳性产物直接进行DNA测序,对检测结果与肿瘤病理分级、肿瘤大小、临床分期等临床指标进行分析.结果 15例RB组织中有6例标本表达NY-ESO-1基因,9例标本表达NY-SAR-35基因;在12例非肿瘤病变视网膜组织及22例眼部正常组织中均未见到NY-ESO-1基因和NY-SAR-35基因的表达.NY-ESO-1基因和NY-SAR-35基因的表达均与患者的性别(P=0.426、0.822)、年龄(P=0.180、0.464)、病理分型(P=0.744、0.582)、肿瘤大小(P=0.760、0.790)、临床分期(P=0.868、0.707)等临床相关指标无明显关联.结论 NY-ESO-1基因及NY-SAR-35基因均可特异性地高表达于RB组织中,其表达率明显高于其他全身肿瘤,有可能成为RB特异性免疫治疗的靶抗原.%Background The immunotherapy for retinoblastoma(RB) is gradually concerned recent year.To seek relative immune-associated antigen is a basis of immunotherapy.NY-ESO-1 and NY-SAR-35 are two kinds of genes of cancer testis antigen( CTA ).To understand their expressions in RB tissue can offer index for relative study.Objective This study was to investigate the expressions of two CTA NY-ESO-1 and NY-SAR-35 in RB and explore the possibility of them as

  7. A novel human-derived antibody against NY-ESO-1 improves the efficacy of chemotherapy.

    Science.gov (United States)

    Gupta, Anurag; Nuber, Natko; Esslinger, Christoph; Wittenbrink, Mareike; Treder, Martin; Landshammer, Alexandro; Noguchi, Takuro; Kelly, Marcus; Gnjatic, Sacha; Ritter, Erika; von Boehmer, Lotta; Nishikawa, Hiroyoshi; Shiku, Hiroshi; Old, Lloyd; Ritter, Gerd; Knuth, Alexander; van den Broek, Maries

    2013-01-01

    We investigated whether antibodies against intracellular tumor-associated antigens support tumor-specific immunity when administered together with a treatment that destroys the tumor. We propose that released antigens form immune complexes with the antibodies, which are then efficiently taken up by dendritic cells. We cloned the first human monoclonal antibodies against the Cancer/Testis (CT) antigen, NY-ESO-1. We tested whether the monoclonal anti-NY-ESO-1 antibody (12D7) facilitates cross-presentation of a NY-ESO-1-derived epitope by dendritic cells to human CD8+ T cells, and whether this results in the maturation of dendritic cells in vitro. We investigated the efficacy of 12D7 in combination with chemotherapy using BALB/c mice bearing syngeneic CT26 tumors that express intracellular NY-ESO-1. Human dendritic cells that were incubated with NY-ESO-1:12D7 immune complexes efficiently stimulated NY-ESO-1(157-165)/HLA-A2-specific human CD8+ T cells to produce interferon-γ, whereas NY-ESO-1 alone did not. Furthermore, the incubation of dendritic cells with NY-ESO-1:12D7 immune complexes resulted in the maturation of dendritic cells. Treatment of BALB/c mice that bear CT26/NY-ESO-1 tumors with 5-fluorouracil (5-FU) plus 12D7 was significantly more effective than chemotherapy alone. We propose systemic injection of monoclonal antibodies (mAbs) against tumor-associated antigens plus a treatment that promotes the local release of those antigens resulting in immune complex formation as a novel therapeutic modality for cancer.

  8. Application of serum NY-ESO-1 antibody assay for early SCLC diagnosis.

    Science.gov (United States)

    Yang, Jihua; Jiao, Shunchang; Kang, Jingbo; Li, Rong; Zhang, Guanzhong

    2015-01-01

    NY-ESO-1 antibody is one of the cancer-related antibodies. The purpose of this study was to investigate the diagnostic role of the NY-ESO-1 humoral immune response in small cell lung cancer (SCLC). We recombined the recombinant protein of NY-ESO-1 antibody and NSE, analyzed them by Enzyme-linked immunosorbent assay, and then established the Receiver Operating Characteristic (ROC) curve to estimate the diagnostic value of NY-ESO-1 antibody, NSE and their combinations. According to detection, the positive rate of NY-ESO-1 humoral immune response (26.3%), NSE (43.8%) and their combinations (10.5%) were all lower than the negative rate which indicated that the NY-ESO-1 antibody might be down-regulated in SCLC. And the positive rate wasn't related to clinicopathologic characteristics. The ROC curve demonstrated that with a 37.17% sensitivity and a 91.7% specificity along with a AUC of 0.619 for NY-ESO-1ab as well as with a 48.3% sensitivity and a 90.87% specificity along with a AUC value of 0.773 for NSE, their diagnostic value were both high. Besides, the diagnostic value of their combinations was also good for a AUC of 0.83 and a 69.12% sensitivity and a 91.8% specificity. There were significant difference of diagnostic value among three types above (NY-ESO-1 vs. NSE, P ESO-1, P ESO-1ab, NSE and their combinations all were important diagnostic markers for SCLC. Moreover, the diagnostic value of their combinations was higher than any single of them. And NY-ESO-1 humoral immune to NSE might be a potential diagnostic indicator in SCLC.

  9. Expression of NY-ESO-1 gene in human hepatocellular carcinoma%人类NY-ESO-1基因编码在肝癌中的表达

    Institute of Scientific and Technical Information of China (English)

    张阳德; 任菲; 彭健

    2013-01-01

    目的 研究NY-ESO-1在肝细胞肝癌中的表达情况并探索其表达与肝癌预后的相关性.方法 我们收集了124例肝癌患者的肝癌组织和相应的癌旁组织,37例肝硬化患者的肝组织,以及43例非肝硬化的肝组织.应用实时荧光定量聚合酶链技术(RT-PCR)检测了上述组织中NY-ESO-1基因的表达水平,并利用DNA免疫杂交技术和测序技术检测RT-PCR产物.收集健康志愿者外周血基因组DNA作为正常对照.结果 在124例肝癌组织中,56例(45.2%)检测到有NY-ESO-1基因的表达,而在癌旁组织中未检测到表达.NY-ESO-1基因的RT-PCR产物长度为329 bp,利用测序方法检测NY-ESO-1基因突变.在56例表达NY-ESO-1的肝癌组织中,有4例检测到位点2的突变.该突变导致一个氨基酸的变异,这种氨基酸的变异对蛋白结构和功能具有决定性的影响.NY-ESO-1基因的表达与临床特征如肿瘤标志物(甲胎蛋白,半乳糖酸苷酶)、转移、复发和肿瘤的大小无显著关联性.结论 NY-ESO-1在肝癌组织中呈高表达,但是与预后无相关性.NY-ESO-1蛋白能否作为肝癌治疗的候选疫苗有待进一步研究.

  10. NY-ESO-1 expression in hepatocellular carcinoma: A potential new marker for early recurrence after surgery.

    Science.gov (United States)

    Xu, Heng; Gu, Na; Liu, Zhao-Bo; Zheng, Min; Xiong, Fang; Wang, Si-Ying; Li, Ning; Lu, Jun

    2012-01-01

    NY-ESO-1 belongs to the cancer testis antigens (CTA) family, and is identified in a variety of tumors. Certain studies have demonstrated that NY-ESO-1 predicts tumor recurrence and treatment response. No reports are currently available regarding the correlation between NY-ESO-1 and the recurrence of hepatocellular carcinoma (HCC) following surgery. The purpose of the present study was to evaluate the association between NY-ESO-1 and relapse of HCC and to explore the possible mechanisms for this correlation. A total of 120 HCC patients were analyzed for the expression of NY-ESO-1 by immunohistochemistry (IHC). A stable NY-ESO-1 over-expressed HepG2 cell line (ESO-HepG2) was established to determine the biological effects of NY-ESO-1 on cell proliferation, cell cycle and migration by using the xCELLigence DP system, flow cytometry and xCELLigence SP system. NY-ESO-1 was positive in 28 of 120 (23.3%) HCC tumor tissues. NY-ESO-1 was not detectable in adjacent normal liver tissues. A close correlation was found between NY-ESO-1 expression and the recurrence of HCC following surgery (P=0.007). Kaplan-Meier analysis showed a shorter recurrence-free survival (RFS) for patients positive for NY-ESO-1 (log-rank test, P=0.003). The Cox regression model demonstrated that NY-ESO-1 expression was a significant independent predictor for the recurrence of HCC following curative surgery (P=0.022). Compared with HepG2 cells, ESO-HepG2 cells have increased migration but not proliferation ability. In conclusion, NY-ESO-1 expression is associated with worse HCC outcome following surgery, and the mechanism for this finding may be that NY-ESO-1 increases tumor cell migration.

  11. THERMOMECHANICAL ANALYSIS OF ILLITE FROM FÜZÉRRADVÁNY

    Directory of Open Access Journals (Sweden)

    Tomáš Húlan

    2015-09-01

    Full Text Available Young’s modulus of green illite from Füzérradvány (Hungary was measured in-situ in the temperature interval 20 °C – 1100 °C and auxiliary analyses DTA, TG and TDA, XRD and EGA, were also performed. It was found that a removal of the physically bound water (20 °C – 250 °C sets illite crystals closer which leads to a significant increase of Young’s modulus from its initial value of 7 GPa and reaches the maximum 12 GPa at 300 °C. Young’s modulus slightly decreases in the temperature interval of dehydroxylation of illite (300 °C – 800 °C, and which then reaches up to 45 GPa at 1100 °C, increasing exponentially as a consequence of the sintering above 800 °C.DOI: http://dx.doi.org/10.5755/j01.ms.21.3.7152

  12. 肿瘤抗原NY-ESO-1研究进展

    Institute of Scientific and Technical Information of China (English)

    王运伟; 顾琴龙; 朱正纲

    2005-01-01

    肿瘤抗原NY-ESO-1是肿瘤-睾丸(cancer-testis,CT)抗原,其编码基因是一基因家族,目前发现的主要包括NY-ESO-1、LAGE-1和ESO3三个成员.NY-ESO-1是迄今发现的最具免疫原性的肿瘤抗原之一,可以在NY-ESO-1表达阳性的肿瘤患者体内引起自发性体液免疫反应和T细胞免疫反应,因此在抗原特异性肿瘤免疫治疗中具有广泛的应用前景.

  13. EnviroAtlas - New York, NY - One Meter Resolution Urban Land Cover Data (2008) Web Service

    Data.gov (United States)

    U.S. Environmental Protection Agency — This EnviroAtlas web service supports research and online mapping activities related to EnviroAtlas (https://www.epa.gov/enviroatlas ). The New York, NY EnviroAtlas...

  14. EnviroAtlas - New York, NY - Meter-Scale Urban Land Cover (MULC) Data (2008)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The New York, NY EnviroAtlas Meter-scale Urban Land Cover (MULC) Data were generated by the University of Vermont Spatial Analysis Laboratory (SAL) under the...

  15. LiDAR Elevation Data Collection - Putnam County, NY, 2008 (NYSDEC)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Summary of the surface elevation data collection project in Putnam County, NY (NYSDEC) 2008. Products generated include LiDAR point data in LAS Binary format v1.1....

  16. 76 FR 4078 - Television Broadcasting Services; North Pole and Plattsburgh, NY

    Science.gov (United States)

    2011-01-24

    ... From the Federal Register Online via the Government Publishing Office FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 Television Broadcasting Services; North Pole and Plattsburgh, NY AGENCY: Federal... CFR Part 73 Television, Television broadcasting. For the reasons discussed in the preamble,...

  17. EnviroAtlas - New York, NY - One Meter Resolution Urban Land Cover Data (2008)

    Data.gov (United States)

    U.S. Environmental Protection Agency — The New York, NY EnviroAtlas Meter-scale Urban Land Cover (MULC) Data were generated by the University of Vermont Spatial Analysis Laboratory (SAL) under the...

  18. Advances in the Research of Tumor Antigen NY-ESO-1%肿瘤抗原NY-ESO-1研究进展

    Institute of Scientific and Technical Information of China (English)

    高丹

    2008-01-01

    NY-ESO-1是肿瘤-睾丸(cancer-testis,CT)抗原家族成员之一,在许多不同类型的人类肿瘤中都有表达,是迄今发现的最具免疫原性的肿瘤抗原之一.NY-ESO-1可以在阳性表达肿瘤患者体内诱导自发性体液和T细胞免疫应答,因此在肿瘤疫苗研制中具有非常广泛的前景.

  19. T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma

    OpenAIRE

    Singh, Nathan; Kulikovskaya, Irina; Barrett, David M.; Binder-Scholl, Gwendolyn; Jakobsen, Bent; Martinez, Daniel; Pawel, Bruce; June, Carl H.; Kalos, Michael D.; Grupp, Stephan A.

    2015-01-01

    The cancer-testis antigen NY-ESO-1 is expressed by many solid tumors and has limited expression by mature somatic tissues, making it a highly attractive target for tumor immunotherapy. Targeting NY-ESO-1 using engineered T cells has demonstrated clinical efficacy in the treatment of some adult tumors. Neuroblastoma is a significant cause of cancer mortality in children, and is a tumor type shown to be responsive to immunotherapies. We evaluated a large panel of primarily resected neuroblastom...

  20. NY-ESO-1 expression is tightly linked to TMPRSS2-ERG fusion in prostate cancer.

    Science.gov (United States)

    Grupp, Katharina; Ospina-Klinck, Daniel; Tsourlakis, Maria Christina; Koop, Christina; Wilczak, Waldemar; Adam, Meike; Simon, Ronald; Sauter, Guido; Izbicki, Jakob Robert; Graefen, Markus; Huland, Hartwig; Steurer, Stefan; Schlomm, Thorsten; Minner, Sarah; Quaas, Alexander

    2014-07-01

    NY-ESO-1 has been suggested as therapeutic cancer vaccine in prostate cancer. This study was undertaken to explore the relationship of NY-ESO-1 with tumor phenotype, biochemical recurrence, and molecular subgroups in hormone-naive prostate cancers. NY-ESO-1 immunohistochemistry was analyzed on a tissue microarray containing 11,152 prostate cancer samples. Results were compared to clinically follow-up data, ERG status, and deletions on PTEN, 3p13, 5q21, and 6q15. NY-ESO-1 expression was absent in benign prostate glands. In prostate cancer, NY-ESO-1 positivity was found 8.8% of our 8,761 interpretable tumors including 5.8% with weak, 2.5% with moderate, and 0.5% with strong expression. There was a threefold higher rate of NY-ESO-1 expression in ERG fusion positive tumors than in ERG negative cancers (P ESO-1 expression was associated with early biochemical recurrence (P = 0.0002) and high Gleason grade (P ESO-1 expression was also linked to PTEN (P = 0.0012) and 6q15 deletions (P = 0.0005). Our observations indicate a tight link of NY-ESO-1 expression to ERG activation and (to a lesser extent) PTEN- and 6q15-deletions in prostate cancer. The impact of these interactions on the likelihood of response to immunotherapy is unclear. The prognostic impact of NY-ESO-1 expression is little and not independent of histologic variables. © 2014 Wiley Periodicals, Inc.

  1. Diagnostic and therapeutic relevance of NY-ESO-1 expression in oral squamous cell carcinoma.

    Science.gov (United States)

    Ries, Jutta; Mollaoglu, Nur; Vairaktaris, Eleftherios; Neukam, Friedrich W; Nkenke, Emeka

    2009-12-01

    Cancer/testis antigen 1B (NY-ESO-1) is exclusively expressed in various types of tumor but not in healthy normal tissue, except testis, and induces strong cellular and humoral immune responses. Therefore, it represents an ideal target for diagnostic and immunotherapeutic applications. The aim of the study was to investigate the expression of NY-ESO-1 in oral squamous cell carcinoma (OSCC) to determine its impact as a diagnostic parameter or a therapeutic target for oral cancer. A total of 65 OSCC and 20 normal oral mucosal samples of otherwise healthy volunteers were included in this study. Expression of NY-ESO-1 was determined using reverse transcriptase polymerase chain reaction (RT-PCR). The results were correlated to diagnosis and clinicopathological parameters. NY-ESO-1 was expressed in 27.7% of the investigated tumor samples, but not in normal oral mucosal. The correlation between NY-ESO-1 expression and malignancy was significant (p=0.008). The prevalence of NY-ESO-1 expression was significantly associated with tumor size (p=0.033), but not with histological grading, positive lymph node status or clinical stage of disease. NY-ESO-1 expression is restricted to OSCC, clearly indicating malignancy. However, the expression rate of this antigen is too low for clinical application but it might be a useful additional biomarker within a multiple marker system for the diagnosis of OSCC. In addition, NY-ESO-1 might be a candidate for immunotherapy and polyvaccination in patients suffering from OSCC.

  2. Three novel NY-ESO-1 epitopes bound to DRB1*0803, DQB1*0401 and DRB1*0901 recognized by CD4 T cells from CHP-NY-ESO-1-vaccinated patients.

    Science.gov (United States)

    Mizote, Yu; Taniguchi, Taku; Tanaka, Kei; Isobe, Midori; Wada, Hisashi; Saika, Takashi; Kita, Shoichi; Koide, Yukari; Uenaka, Akiko; Nakayama, Eiichi

    2010-07-19

    Three novel NY-ESO-1 CD4 T cell epitopes were identified using PBMC obtained from patients who were vaccinated with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein (CHP-NY-ESO-1). The restriction molecules were determined by antibody blocking and using various EBV-B cells with different HLA alleles as APC to present peptides to CD4 T cells. The minimal epitope peptides were determined using various N- and C-termini truncated peptides deduced from 18-mer overlapping peptides originally identified for recognition. Those epitopes were DRB1*0901-restricted NY-ESO-1 87-100, DQB1*0401-restricted NY-ESO-1 95-107 and DRB1*0803-restricted NY-ESO-1 124-134. CD4 T cells used to determine those epitope peptides recognized EBV-B cells or DC that were treated with recombinant NY-ESO-1 protein or NY-ESO-1-expressing tumor cell lysate, suggesting that the epitope peptides are naturally processed. These CD4 T cells showed a cytokine profile with Th1 characteristics. Furthermore, NY-ESO-1 87-100 peptide/HLA-DRB1*0901 tetramer staining was observed. Multiple Th1-type CD4 T cell responses are beneficial for inducing effective anti-tumor responses after NY-ESO-1 protein vaccination. (c) 2010 Elsevier Ltd. All rights reserved.

  3. NY-ESO-1 (CTAG1B) expression in mesenchymal tumors.

    Science.gov (United States)

    Endo, Makoto; de Graaff, Marieke A; Ingram, Davis R; Lim, Simin; Lev, Dina C; Briaire-de Bruijn, Inge H; Somaiah, Neeta; Bovée, Judith V M G; Lazar, Alexander J; Nielsen, Torsten O

    2015-04-01

    New York esophageal squamous cell carcinoma 1 (NY-ESO-1, CTAG1B) is a cancer-testis antigen and currently a focus of several targeted immunotherapeutic strategies. We performed a large-scale immunohistochemical expression study of NY-ESO-1 using tissue microarrays of mesenchymal tumors from three institutions in an international collaboration. A total of 1132 intermediate and malignant and 175 benign mesenchymal lesions were enrolled in this study. Immunohistochemical staining was performed on tissue microarrays using a monoclonal antibody for NY-ESO-1. Among mesenchymal tumors, myxoid liposarcomas showed the highest positivity for NY-ESO-1 (88%), followed by synovial sarcomas (49%), myxofibrosarcomas (35%), and conventional chondrosarcomas (28%). Positivity of NY-ESO-1 in the remaining mesenchymal tumors was consistently low, and no immunoreactivity was observed in benign mesenchymal lesions. On the basis of these findings, nearly 90% of myxoid liposarcomas, as well as a significant proportion of synovial sarcomas, myxofibrosarcomas, and conventional chondrosarcomas are good candidates for immunotherapy targeting NY-ESO-1.

  4. NY-ESO-1 as a potential immunotherapeutic target in renal cell carcinoma.

    Science.gov (United States)

    Giesen, Eva; Jilaveanu, Lucia B; Parisi, Fabio; Kluger, Yuval; Camp, Robert L; Kluger, Harriet M

    2014-07-30

    Novel immune therapies targeting tumor specific antigens are being developed. Our purpose was to determine expression of the cancer testes antigen NY-ESO-1 in renal cell carcinoma (RCC), as NY-ESO-1 targeting approaches, particularly adoptive cell therapy, have not been evaluated in this disease. We employed tissue microarrays containing >300 unique RCC cases and adjacent benign renal tissue to determine NY-ESO-1 expression using a quantitative immunofluorescence method. In addition, we studied NY-ESO-1 expression in 35 matched primary and metastatic RCC specimens to assess concordance between different tumor sites. NY-ESO-1 was highly expressed in a subset of RCCs. Expression in primary RCC specimens was significantly higher than adjacent normal renal tissue (PESO-1 expression seen in clear cell RCC suggests that NY-ESO-1 targeting approaches should be studied in this disease. Expression is higher in metastatic sites, and discordance between primary and metastatic sites in some patients suggests that patient selection for these therapies should be based on expression in metastatic rather than nephrectomy specimens.

  5. Atmospheric Research in Ny-Aalesund. Proceedings from the Third NySMAC meeting. NILU, Kjeller, Norway, 9.-11. April 1997

    Energy Technology Data Exchange (ETDEWEB)

    Floeisand, Inga; Gernandt, Hartwig; Larsen, Elisabeth Stoltz; Stordal, Frode; Wada, Makoto

    1997-07-01

    Leading abstract. The Ny-Aalesund Seminar Series was conceived by NySMAC (Ny-Aalesund Science Managers Committee) as a means of bringing together research scientists involved in programmes running at Ny-Aalesund or having an interest in that area. The first Seminar was held in Potsdam in Germany in May 1995, and included talks which covered all current research disciplines; physical, atmospheric, earth and biological sciences. The Second Seminar was devoted to biological science and particularly to ecology. It was held in Cambridge in UK in February 1996. The subject of this Third Seminar, held in Kjeller in Norway in April 1997, is atmospheric research in Ny-Aalesund. Since the early 1980s a wide range of projects have been carried out on Svalbard and particularly at Ny-Aalesund. Prominent in this research have been several international research programmes with emphasis on topics of e.g. Arctic (Arctic Monitoring and Assessment Programme, AMAP), European (European Monitoring and Evaluation Programme, EMEP) or global (International Geosphere-Biosphere Programme, IGBP; World Climate Research Programme, WCRP) scales. Atmospheric observations have been carried out in Ny-Aalesund in Svalbard for several years. From the early 1980s, the long range transport of sulphur dioxide and sulphate aerosols leading to Arctic Haze, has been studied. As a consequence, EMEP included Ny-Aalesund in their network. In the following years the transport of ozone and chemical precursors were observed, and recently the naturally occurring phenomenon of rapid surface ozone loss has been given particular attention. From the late 1980s observations of the stratospheric ozone layer and related chemical species were undertaken, and the efforts have increased considerably since. Ny-Aalesund is now one of the primary Arctic sites of the NDSC (Network for Detection of Stratospheric Change) network. Important efforts have been undertaken to participate in the European campaigns as the European

  6. Dept. of Agricultural Economics & Extension

    African Journals Online (AJOL)

    budget deficits and stimulated export production, concerns have been raised with ..... are those whose welfare falls below the lower poverty line (Ghana. Statistical ..... training should also be given to help them better manage their incomes; '.

  7. [Prokaryotic expression, purification and identification of NY-ESO-1/GST fusion protein in E.coli].

    Science.gov (United States)

    Tang, Lei; Song, Chao-jun; Sun, Yuan-jie; Li, Na; Wei, Yu-ying; Sun, Yi; Yang, Kun

    2012-10-01

    To construct an expression plasmid for NY-ESO-1 gene and identify the expression of recombinant protein NY-ESO-1/GST in E.coli. NY-ESO-1 segment was amplified from the testis cDNA library by RT-PCR and cloned into the prokaryotic expression vector pGEX4T-1 downstream tagged by GST to construct the expression plasmid pGEX-4T1-NY-ESO-1. The recombinant vector was transformed to BL21 (DE3) and NY-ESO-1/GST fusion protein was induced expression by IPTG. The protein was purified by urea elution and identified by SDS-PAGE and Western blotting. The NY-ESO-1 segment was successfully amplified and its sequence was identical with that published in GenBank. The BL21 (DE3) pLysS containing the pGEX-4T1-NY-ESO-1 expressed a M(r); 44 000 fusion protein under the induction of IPTG. The purity of the protein was 90%. Western blotting proved that NY-ESO-1/GST had a specific reaction with anti-GST mAb. The prokaryotic expression vector of NY-ESO-1 has been constructed and the fusion protein NY-ESO-1/GST of high purity is successfully expressed.

  8. Antibody responses against NY-ESO-1 and HER2 antigens in patients vaccinated with combinations of cholesteryl pullulan (CHP)-NY-ESO-1 and CHP-HER2 with OK-432.

    Science.gov (United States)

    Aoki, Masatoshi; Ueda, Shugo; Nishikawa, Hiroyoshi; Kitano, Shigehisa; Hirayama, Michiko; Ikeda, Hiroaki; Toyoda, Hideki; Tanaka, Kyosuke; Kanai, Michiyuki; Takabayashi, Arimichi; Imai, Hiroshi; Shiraishi, Taizo; Sato, Eiichi; Wada, Hisashi; Nakayama, Eiichi; Takei, Yoshiyuki; Katayama, Naoyuki; Shiku, Hiroshi; Kageyama, Shinichi

    2009-11-16

    Combination vaccines of the NY-ESO-1 protein complexed with cholesteryl pullulan (CHP), CHP-NY-ESO-1, and the truncated 146HER2 protein with CHP, CHP-HER2, were subcutaneously administered with the immuno-adjuvant OK-432 to eight esophageal cancer patients. Vaccination was well-tolerated. NY-ESO-1- and HER2-specific antibody responses were analyzed using the patients' sera and samples from previous single CHP-NY-ESO-1 or CHP-HER2 vaccine trial. The responses to NY-ESO-1 in the combination vaccine study were comparable to the single vaccine. For responses to HER2, there were fewer antibody responses in the combination vaccines. Although there were marked individual variations in the antibody responses to the NY-ESO-1 and HER2 antigens, the reaction patterns to these antigens were comparable within each patient. Antibodies to OK-432 were not augmented. Protein cancer vaccines targeting multiple antigens are feasible.

  9. NY-ESO-1 expression in meningioma suggests a rationale for new immunotherapeutic approaches.

    Science.gov (United States)

    Baia, Gilson S; Caballero, Otavia L; Ho, Janelle S Y; Zhao, Qi; Cohen, Tzeela; Binder, Zev A; Salmasi, Vafi; Gallia, Gary L; Quinones-Hinojosa, Alfredo; Olivi, Alessandro; Brem, Henry; Burger, Peter; Strausberg, Robert L; Simpson, Andrew J G; Eberhart, Charles G; Riggins, Gregory J

    2013-11-01

    Meningiomas are the most common primary intracranial tumors. Surgical resection remains the treatment of choice for these tumors. However, a significant number of tumors are not surgically accessible, recur, or become malignant, necessitating the repetition of surgery and sometimes radiation. Chemotherapy is rarely used and is generally not recognized as an effective treatment. Cancer/testis (CT) genes represent a unique class of genes, which are expressed by germ cells, normally silenced in somatic cells, but activated in various cancers. CT proteins can elicit spontaneous immune responses in patients with cancer and this feature makes them attractive targets for immunotherapy-based approaches. We analyzed mRNA expression of 37 testis-restricted CT genes in a discovery set of 18 meningiomas by reverse transcription PCR. The overall frequency of expression of CT genes ranged from 5.6% to 27.8%. The most frequently expressed was NY-ESO-1, in 5 patients (27.8%). We subsequently analyzed NY-ESO-1 protein expression in a larger set of meningiomas by immunohistochemistry and found expression in 108 of 110 cases. In some cases, NY-ESO-1 expression was diffused and homogenous, but in most instances it was heterogeneous. Importantly, NY-ESO-1 expression was positively correlated with higher grade and patients presenting with higher levels of NY-ESO-1 staining had significantly worse disease-free and overall survival. We have also shown that NY-ESO-1 expression may lead to humoral immune response in patients with meningioma. Considering the limited treatment options for patients with meningioma, the potential of NY-ESO-1-based immunotherapy should be explored. ©2013 AACR.

  10. Efficacy of systemic adoptive transfer immunotherapy targeting NY-ESO-1 for glioblastoma.

    Science.gov (United States)

    Everson, Richard G; Antonios, Joseph P; Lisiero, Dominique N; Soto, Horacio; Scharnweber, Rudi; Garrett, Matthew C; Yong, William H; Li, Ning; Li, Gang; Kruse, Carol A; Liau, Linda M; Prins, Robert M

    2016-03-01

    Immunotherapy is an ideal treatment modality to specifically target the diffusely infiltrative tumor cells of malignant gliomas while sparing the normal brain parenchyma. However, progress in the development of these therapies for glioblastoma has been slow due to the lack of immunogenic antigen targets that are expressed uniformly and selectively by gliomas. We utilized human glioblastoma cell cultures to induce expression of New York-esophageal squamous cell carcinoma (NY-ESO-1) following in vitro treatment with the demethylating agent decitabine. We then investigated the phenotype of lymphocytes specific for NY-ESO-1 using flow cytometry analysis and cytotoxicity against cells treated with decitabine using the xCelligence real-time cytotoxicity assay. Finally, we examined the in vivo application of this immune therapy using an intracranially implanted xenograft model for in situ T cell trafficking, survival, and tissue studies. Our studies showed that treatment of intracranial glioma-bearing mice with decitabine reliably and consistently induced the expression of an immunogenic tumor-rejection antigen, NY-ESO-1, specifically in glioma cells and not in normal brain tissue. The upregulation of NY-ESO-1 by intracranial gliomas was associated with the migration of adoptively transferred NY-ESO-1-specific lymphocytes along white matter tracts to these tumors in the brain. Similarly, NY-ESO-1-specific adoptive T cell therapy demonstrated antitumor activity after decitabine treatment and conferred a highly significant survival benefit to mice bearing established intracranial human glioma xenografts. Transfer of NY-ESO-1-specific T cells systemically was superior to intracranial administration and resulted in significantly extended and long-term survival of animals. These results reveal an innovative, clinically feasible strategy for the treatment of glioblastoma. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Neuro

  11. Heteroclitic serological response in esophageal and prostate cancer patients after NY-ESO-1 protein vaccination.

    Science.gov (United States)

    Kawada, Junji; Wada, Hisashi; Isobe, Midori; Gnjatic, Sacha; Nishikawa, Hiroyoshi; Jungbluth, Achim A; Okazaki, Nami; Uenaka, Akiko; Nakamura, Yurika; Fujiwara, Shinichi; Mizuno, Naoaki; Saika, Takashi; Ritter, Erika; Yamasaki, Makoto; Miyata, Hiroshi; Ritter, Gerd; Murphy, Roger; Venhaus, Ralph; Pan, Linda; Old, Lloyd J; Doki, Yuichiro; Nakayama, Eiichi

    2012-02-01

    NY-ESO-1 is a prototypic cancer/testis antigen. In a recent phase I clinical trial, we vaccinated 13 patients bearing NY-ESO-1-expressing tumors with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein (CHP-NY-ESO-1) and showed efficient induction of NY-ESO-1 antibody, and CD4 and CD8 T cell responses using peripheral blood from the patients. In our study, we analyzed heteroclitic serological responses in those patients after vaccination. Serological response against 11 tumor antigens including MAGE-A1, MAGE-A3, MAGE-A4, CT7/MAGEC1, CT10/MAGEC2, CT45, CT46/HORMAD1, SOX2, SSX2, XAGE1B and p53 was examined by enzyme-linked immunosorbent assay (ELISA) using sera from ten vaccinated patients. Expression of tumor antigens was determined by reverse transcription-polymerase chain reaction or immunohistochemistry. Eight of nine patients who showed antibody responses against NY-ESO-1 also showed an antibody response against at least 1 of these 11 tumor antigens after vaccination. In one patient, seven tumor antigens were recognized. Specificity analysis of the antibody response by ELISA using control recombinant proteins and synthetic peptides and by Western blot showed that the response was not against His6-tag and/or bacterial products included in a preparation of CHP-NY-ESO-1 used for vaccination. Thus, heteroclitic serological responses appear to be indicative of the overall immune response against the tumor, and their analysis could be useful for immune monitoring in cancer vaccine. Copyright © 2011 UICC.

  12. CTLA-4 blockade enhances polyfunctional NY-ESO-1 specific T cell responses in metastatic melanoma patients with clinical benefit.

    Science.gov (United States)

    Yuan, Jianda; Gnjatic, Sacha; Li, Hao; Powel, Sarah; Gallardo, Humilidad F; Ritter, Erika; Ku, Geoffrey Y; Jungbluth, Achim A; Segal, Neil H; Rasalan, Teresa S; Manukian, Gregor; Xu, Yinyan; Roman, Ruth-Ann; Terzulli, Stephanie L; Heywood, Melanie; Pogoriler, Evelina; Ritter, Gerd; Old, Lloyd J; Allison, James P; Wolchok, Jedd D

    2008-12-23

    Blockade of inhibitory signals mediated by cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) has been shown to enhance T cell responses and induce durable clinical responses in patients with metastatic melanoma. The functional impact of anti-CTLA-4 therapy on human immune responses is still unclear. To explore this, we analyzed immune-related adverse events and immune responses in metastatic melanoma patients treated with ipilimumab, a fully human anti-CTLA-4 monoclonal antibody. Fifteen patients were selected on the basis of availability of suitable specimens for immunologic monitoring, and eight of these showed evidence of clinical benefit. Five of the eight patients with evidence of clinical benefit had NY-ESO-1 antibody, whereas none of seven clinical non-responders was seropositive for NY-ESO-1. All five NY-ESO-1 seropositive patients had clearly detectable CD4(+) and CD8(+) T cells against NY-ESO-1 following treatment with ipilimumab. One NY-ESO-1 seronegative clinical responder also had a NY-ESO-1 CD4(+) and CD8(+) T cell response, possibly related to prior vaccination with NY-ESO-1. Among five clinical non-responders analyzed, only one had a NY-ESO-1 CD4(+) T cell response and this patient did not have detectable anti-NY-ESO-1 antibody. Overall, NY-ESO-1-specific T cell responses increased in frequency and functionality during anti-CTLA-4 treatment, revealing a polyfunctional response pattern of IFN-gamma, MIP-1beta and TNF-alpha. We therefore suggest that CTLA-4 blockade enhanced NY-ESO-1 antigen-specific B cell and T cell immune responses in patients with durable objective clinical responses and stable disease. These data provide an immunologic rationale for the efficacy of anti-CTLA-4 therapy and call for immunotherapeutic designs that combine NY-ESO-1 vaccination with CTLA-4 blockade.

  13. The distribution of depleted uranium contamination in Colonie, NY, USA

    Energy Technology Data Exchange (ETDEWEB)

    Lloyd, N.S., E-mail: nsl3@alumni.leicester.ac.uk [Department of Geology, University of Leicester, University Road, Leicester, LE1 7RH (United Kingdom); Chenery, S.R.N. [British Geological Survey, Kingsley Dunham Centre, Keyworth, Nottingham, NG12 5GG (United Kingdom); Parrish, R.R. [Department of Geology, University of Leicester, University Road, Leicester, LE1 7RH (United Kingdom); NERC Isotope Geosciences Laboratory, Kingsley Dunham Centre, Keyworth, Nottingham, NG12 5GG (United Kingdom)

    2009-12-20

    Uranium oxide particles were dispersed into the environment from a factory in Colonie (NY, USA) by prevailing winds during the 1960s and '70s. Uranium concentrations and isotope ratios from bulk soil samples have been accurately measured using inductively coupled plasma quadrupole mass spectrometry (ICP-QMS) without the need for analyte separation chemistry. The natural range of uranium concentrations in the Colonie soils has been estimated as 0.7-2.1 {mu}g g{sup -1}, with a weighted geometric mean of 1.05 {mu}g g{sup -1}; the contaminated soil samples comprise uranium up to 500 {+-} 40 {mu}g g{sup -1}. A plot of {sup 236}U/{sup 238}U against {sup 235}U/{sup 238}U isotope ratios describes a mixing line between natural uranium and depleted uranium (DU) in bulk soil samples; scatter from this line can be accounted for by heterogeneity in the DU particulate. The end-member of DU compositions aggregated in these bulk samples comprises (2.05 {+-} 0.06) x 10{sup -3235}U/{sup 238}U, (3.2 {+-} 0.1) x 10{sup -5236}U/{sup 238}U, and (7.1 {+-} 0.3) x 10{sup -6234}U/{sup 238}U. The analytical method is sensitive to as little as 50 ng g{sup -1} DU mixed with the natural uranium occurring in these soils. The contamination footprint has been mapped northward from site, and at least one third of the uranium in a soil sample from the surface 5 cm, collected 5.1 km NNW of the site, is DU. The distribution of contamination within the surface soil horizon follows a trend of exponential decrease with depth, which can be approximated by a simple diffusion model. Bioturbation by earthworms can account for dispersal of contaminant from the soil surface, in the form of primary uranium oxide particulates, and uranyl species that are adsorbed to organic matter. Considering this distribution, the total mass of uranium contamination emitted from the factory is estimated to be c. 4.8 tonnes.

  14. NY-ESO-1 expression in hepatocellular carcinoma: A potential new marker for early recurrence after surgery

    OpenAIRE

    Xu, Heng; GU, NA; LIU, ZHAO-BO; Zheng, Min; Xiong, Fang; WANG, SI-YING; Li, Ning; Lu, Jun

    2011-01-01

    NY-ESO-1 belongs to the cancer testis antigens (CTA) family, and is identified in a variety of tumors. Certain studies have demonstrated that NY-ESO-1 predicts tumor recurrence and treatment response. No reports are currently available regarding the correlation between NY-ESO-1 and the recurrence of hepatocellular carcinoma (HCC) following surgery. The purpose of the present study was to evaluate the association between NY-ESO-1 and relapse of HCC and to explore the possible mechanisms for th...

  15. NY-ESO-1 expression in hepatocellular carcinoma: A potential new marker for early recurrence after surgery

    OpenAIRE

    2011-01-01

    NY-ESO-1 belongs to the cancer testis antigens (CTA) family, and is identified in a variety of tumors. Certain studies have demonstrated that NY-ESO-1 predicts tumor recurrence and treatment response. No reports are currently available regarding the correlation between NY-ESO-1 and the recurrence of hepatocellular carcinoma (HCC) following surgery. The purpose of the present study was to evaluate the association between NY-ESO-1 and relapse of HCC and to explore the possible mechanisms for th...

  16. Estimation of an NY-ESO-1 expressing HCC cell line by NY-ESO-1b specific CD8+T cells in vitro induced by HLA-A2 restricted NY-ESO-1b peptide%HLA-A2限制性NY-ESO-1b肽段诱导的CD8+ T细胞对稳定表达NY-ESO-1的HepG2肝癌细胞系的杀伤效应

    Institute of Scientific and Technical Information of China (English)

    乔欢; 钱晓萍; 张华刚; 田婵; 陈慰峰

    2005-01-01

    目的:分析体外诱导的NY-ESO-1特异性杀伤性T淋巴细胞对稳定表达NY-ESO-1蛋白的肝细胞性肝癌(hepatocellular carcinoma,HCC)细胞系的杀伤效应,为进一步分析NY-ESO-1蛋白作为疫苗用于治疗HCC的可能性提供实验支持.方法:从HLA-A2阳性的肝癌患者体内分离出外周血单个核细胞,用NY-ESO-1肽段体外诱导特异性杀伤性T淋巴细胞,并通过酶联免疫斑点法分析特异性杀伤性T淋巴细胞对稳定表达NY-ESO-1蛋白的HCC细胞系HepG2细胞的杀伤效应.结果:转染了NY-ESO-1的HepG2细胞(HLA-A2+)可有效地被NY-ESO-1157-165抗原肽诱导的特异性CD8+ T淋巴细胞所识别.效应细胞中大量GrB的释放表明其对稳定转染了NY-ESO-1基因的HepG2细胞有很强的杀伤作用.结论:HepG2细胞中表达的NY-ESO-1蛋白可被此肝癌细胞有效地加工处理,而且HLA-A2限制性的抗原肽NY-ESO-1157-165也可被有效地提呈到细胞表面,从而被效应T细胞所识别.

  17. Middle Permian palaeobiogeography study in East Kunlun, A'nyêmaqên and Bayan Har

    Institute of Scientific and Technical Information of China (English)

    WANG; Yongbiao; YANG; Hao

    2004-01-01

    Three regions can easily be identified in the study area according to the Middle Permian palaeobiogeographic distribution of biota, they are the southern slope of East Kunlun, A'nyêmaqên and Bayan Har. Biotic constitution and ecology in the southern slope of East Kunlun and Bayan Har are very similar. Both the diversity and abundance of organisms in these two areas are very high and reefs are widely developed. However, biotic diversity and abundance in A'nyêmaqên which is between the above two areas are obviously low. Differentiation of palaeobiogeographic distribution in these areas should be due to the baring of A'nyêmaqên ocean in the time of Middle Permian. Middle Permian radiolarian chert and thick abyssal red ooze are widely spread in A'nyêmaqên, implying that the A'nyêmaqên ocean had a great scale in size. Vast scale of deep ocean basin became an impassable gulf for some of the benthos, and as a result, only part of the organisms could have the chance to get to the isolated islands situated in ocean basin. Small living space and hard conditions in the islands further limited the abundance and diversity of biota. Tectonic background reflected by the geochemical study of basalt in the three areas is coupling well enough with the palaeobiogeographic division.

  18. Short-term Climate Characteristics at Ny-(A)lesund over the Arctic Tundra Area

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Based on the Germany Koldwey Station's 1994-2003 conventional observation hourly data, this paper conducts a statistical analysis on the short-term climate characteristics for an arctic tundra region (Ny-(A)lesund island) where our first arctic expedition station (Huanghe Station) was located. Affected by the North Atlantic warming current, this area has a humid temperate climate, and the air temperature at Ny-(A)lesund rose above 0 ℃ even during deep winter season during our research period. The wind speed in this area was low and appeared most at southeast direction. We find that the temperature at Ny-(A)lesund rose in the faster rate (0.68 ℃/10 a) than those at the whole Arctic area. Compared with the floating ices where our expedition conducted in the Arctic, Ny-(A)lesund was warmer and more humid and had lower wind speed. Comparison of the near surface air temperature derived by NCEP/NCAR reanalysis to the conventional measurements conducted at the Koldwey site in Ny-(A)lesund area shows a good agreement for winter season and a significant difference for summer season.

  19. A phase I study of vaccination with NY-ESO-1f peptide mixed with Picibanil OK-432 and Montanide ISA-51 in patients with cancers expressing the NY-ESO-1 antigen.

    Science.gov (United States)

    Kakimi, Kazuhiro; Isobe, Midori; Uenaka, Akiko; Wada, Hisashi; Sato, Eiichi; Doki, Yuichiro; Nakajima, Jun; Seto, Yasuyuki; Yamatsuji, Tomoki; Naomoto, Yoshio; Shiraishi, Kenshiro; Takigawa, Nagio; Kiura, Katsuyuki; Tsuji, Kazuhide; Iwatsuki, Keiji; Oka, Mikio; Pan, Linda; Hoffman, Eric W; Old, Lloyd J; Nakayama, Eiichi

    2011-12-15

    We conducted a phase I clinical trial of a cancer vaccine using a 20-mer NY-ESO-1f peptide (NY-ESO-1 91-110) that includes multiple epitopes recognized by antibodies, and CD4 and CD8 T cells. Ten patients were immunized with 600 μg of NY-ESO-1f peptide mixed with 0.2 KE Picibanil OK-432 and 1.25 ml Montanide ISA-51. Primary end points of the study were safety and immune response. Subcutaneous injection of the NY-ESO-1f peptide vaccine was well tolerated. Vaccine-related adverse events observed were fever (Grade 1), injection-site reaction (Grade 1 or 2) and induration (Grade 2). Vaccination with the NY-ESO-1f peptide resulted in an increase or induction of NY-ESO-1 antibody responses in nine of ten patients. The sera reacted with recombinant NY-ESO-1 whole protein as well as the NY-ESO-1f peptide. An increase in CD4 and CD8 T cell responses was observed in nine of ten patients. Vaccine-induced CD4 and CD8 T cells responded to NY-ESO-1 91-108 in all patients with various HLA types with a less frequent response to neighboring peptides. The findings indicate that the 20-mer NY-ESO-1f peptide includes multiple epitopes recognized by CD4 and CD8 T cells with distinct specificity. Of ten patients, two with lung cancer and one with esophageal cancer showed stable disease. Our study shows that the NY-ESO-1f peptide vaccine was well tolerated and elicited humoral, CD4 and CD8 T cell responses in immunized patients. Copyright © 2011 UICC.

  20. 铜绿假单胞菌NY3所产表面活性剂对原油降解的影响%Effects of rhamnolipid on oil degradation by Pseudomonas aeruginosa strain NY3

    Institute of Scientific and Technical Information of China (English)

    常虹; 蒋欣; 聂麦茜; 葛碧洲; 刘超; 杨琴; 周立辉; 樊晓宇; 孙超; 韦绒绒

    2013-01-01

    铜绿假单胞菌NY3是从石油污染土壤中分离出的一株能快速代谢疏水性化合物的菌种.研究了该菌产表面活性剂及对原油降解的作用.实验表明,在敞开体系中,投加82 mg/L NY3菌产的鼠李糖脂,240 h能使NY3对原油的降解率提高50%.投加甘油使NY3产鼠李糖脂与降解原油同步进行,与投加鼠李糖脂对原油降解的促进作用相近.投加9%甘油使NY3在168 h对原油的降解率提高43%.NY3菌能同时降解原油中的直链烷烃及菲(Pr)和芘(Ph)等多环芳烃.在敞开体系中用少量甘油使产鼠李糖脂和降解原油同步进行,节约处理费用.研究结果为NY3菌株在露天石油污染环境修复中的应用奠定了基础.%Pseudomonas aeruginosa NY3 , being able to metabolize hydrophobic compounds, was isolated from petroleum-contaminated soil samples. Effects of the rhamnolipid and its production on crude oil degradation by NY3 strain were discussed. The results showed that the rhamnolipid produced by NY3 strain could enhance crude oil biodegradation. Comparing with the controls, oil removal rate within 240 h enhanced 50% with 82 mg/ L rhamnolipid. Adding glycerol in NY3 degrading systems, the degradation of crude oil and the rhamnolipid production took place simultaneously, the instantly produced rhamnolipid also accelerated oil metabolism. Adding 9‰ glycerol, the crude oil removal rate by NY3 improved about 43% within 168 h. Phenanthrene (Ph) and py-rene ( Pr) containing in the crude oil could also be removed. The cost of the remediation of contaminated water body by NY3 strain can be decreased by using small amount of glycerol, instead of using rhamnolipid directly. The above results lay a foundation for NY3 strain applying to oil-spill clean-up.

  1. Prokaryotic expression, purification and identification of NY-ESO-1/GST fusion protein in E.coli%NY-ESO-1/GST融合蛋白在大肠杆菌的表达、纯化及鉴定

    Institute of Scientific and Technical Information of China (English)

    汤蕾; 宋朝君; 孙元杰; 李娜; 魏玉英; 孙奕; 杨琨

    2012-01-01

    AIM: To construct an expression plasmid for NY-ESO-1 gene and identify the expression of recombinant protein NY-ESO-1/GST in E. coli. METHODS: NY-ESO-1 segment was amplified from the testis cDNA library by RT-PCR and cloned into the prokaryotic expression vector pGEX4T-l downstream tagged by GST to construct the expression plasmid pGEX4Tl-NY-ESO-l. The recombinant vector was transformed to BL21 ( DE3) and NY-ESO-1/ GST fusion protein was induced expression by IPTG. The protein was purified by urea elution and identified by SDS-PAGE and Western blotting. RESULTS: The NY-ESO-1 segment was successfully amplified and its sequence was identical with that published in GenBank. The BL21 (DE3) pLysS containing the pGEX-Tl-NY-ESO-1 expressed a M, 44 000 fusion protein under the induction of IPTG. The purity of the protein was 90%. Western blotting proved that NY-ESO-1/GST had a specific reaction with anti-GST mAb. CONCLUSION; The prokaryotic expression vector of NY-ESO-1 has been constructed and the fusion protein NY-ESO-1/GST of high purity is successfully expressed.%目的:构建肿瘤-睾丸抗原NY-ESO-1与GST融合基因的原核表达载体,在大肠杆菌中表达并对融合蛋白NY-ESO-1/GST进行纯化和初步鉴定.方法:设计针对NY-ESO-1的特异性引物,采用聚合酶链反应(PCR)从睾丸组织的cDNA文库扩增NY-ESO-1基因片段,经上下游引物所引入的EcoR I和Xho I双酶切后克隆人原核表达载体pGEX-4T1中GST标签的下游,构建重组表达载体pGEX-4T1-NY-ESO-1,并将该载体转化大肠杆菌BL21( DE3) pLysS中,IPTG诱导表达NYESO-1/GST融合蛋白,经不同浓度尿素洗脱纯化后,表达产物通过SDS-PAGE和Western blot法进行鉴定.结果:重组质粒经限制性内切酶EcoR I和Xho I双酶切鉴定和IPTG诱导表达NY-ESO-1/GST的SDS-PAGE分析表明,表达产物的相对分子质量(Mr)为44000,与理论值相符,并主要以包涵体形式存在,灰度扫描分析显示融合蛋白表

  2. 33 CFR 110.84 - Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y.

    Science.gov (United States)

    2010-07-01

    ... of Porter Avenue, Buffalo, N.Y. 110.84 Section 110.84 Navigation and Navigable Waters COAST GUARD... Channel opposite foot of Porter Avenue, Buffalo, N.Y. An area extending northwesterly between Black Rock Channel and Bird Island Pier opposite the foot of Porter Avenue, bounded as follows: Beginning at...

  3. 77 FR 42510 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY; Correction

    Science.gov (United States)

    2012-07-19

    ... National Park Service Notice of Inventory Completion: New York University College of Dentistry, New York... College of Dentistry, New York, NY. The human remains were removed from Bronx County, NY. This notice is... was made by New York University College of Dentistry professional staff in consultation...

  4. 78 FR 62293 - Safety Zone, Oyster Festival 30th Anniversary Fireworks Display, Oyster Bay; Oyster Bay, NY

    Science.gov (United States)

    2013-10-15

    ... CFR Part 165 RIN 1625-AA00 Safety Zone, Oyster Festival 30th Anniversary Fireworks Display, Oyster Bay; Oyster Bay, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the navigable waters of Oyster Bay near Oyster Bay, NY for the...

  5. 78 FR 38310 - Kiwi Energy NY LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request...

    Science.gov (United States)

    2013-06-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Kiwi Energy NY LLC; Supplemental Notice That Initial Market-Based Rate... notice in the above-referenced proceeding, of Kiwi Energy NY LLC's application for market-based...

  6. Transfection of NY-ESO-1 specific TCR improves the specific cytotoxicity of human PBMC to NY-ESO-1 positive tumor cells%转染NY-ESO-1特异性TCR增强人PBMC对NY-ESO-1阳性肿瘤细胞的特异性细胞毒性

    Institute of Scientific and Technical Information of China (English)

    郭佳; 田洲; 顾娜; 徐珩; 闾军

    2013-01-01

    Objective To study whether NY-ESO-1-specific TCR gene transduced into human PBMC (Peripheral Blood Mononuclear Cell, PBMC) can improve its abilities of specifically recognizing and killing tumor cells. Methods pCDNA3. 1-ESO-TCR plasmid was electroporately transferred into PBMC separated from healthy people in vitro and confirmed by RT-PCR. The phenotype analysis after eletroporation was measured by flow cytometry method. The NY-ESO-1 specific peptide (pl57-165) was added into the culture of electroporated PBMC, the IFN-γ level secreted by electroporated PBMC was detected by ELISPOT assay. The specific cytotoxicity in vitro was detected by real time cell analysis (ACEA biosciences). Results The NY-ESO-1-specific TCR fragments in electroporated PBMC were detected by RT-PCR. The specific expression of NY-ESO-TCR in transferred PBMC was significantly higher than that in PBMC transfered with empty vector (P < 0. 05). The positive dots of IFN-γ secretion in transferred PBMC stimulated by peptide pl57-165 was significantly more than that in PBMC transfered with empty vector (P <0. 05); The specific cytotoxicity of transferred PBMC was obviously enhanced than PBMC transduced with empty vector. Conclusions The specific cytotoxicity of PBMC against NY-ESO-1 positive HepG2 cells is elevated by transduced PBMC with NY-ESO-1 specific TCR, which may stimulate more researches about adoptive immunotherapy of hepatocellular carcinoma.%目的 探讨体外转导NY-ESO-1特异性T细胞受体至人外周血淋巴细胞中,是否可增强转导后细胞体外特异性识别并杀伤肿瘤细胞的能力.方法 将NY-ESO-1特异性TCR质粒(pCDNA3.1-ESO-TCR)体外电转入新分离的正常人PBMC中,RT-PCR法鉴定转导是否成功;采用流式细胞仪分析转导后PBMC表型;用特异性NY-ESO-1b抗原肽(p157-165)刺激转导PBMC后,用ELISPOT法检测PBMC分泌IFN-γ的能力,用实时无标记动态细胞分析仪检测PBMC特异性细胞毒性作用.结果 电转后PBMC

  7. A potential role for immunotherapy in thyroid cancer by enhancing NY-ESO-1 cancer antigen expression.

    Science.gov (United States)

    Gunda, Viswanath; Frederick, Dennie T; Bernasconi, Maria J; Wargo, Jennifer A; Parangi, Sareh

    2014-08-01

    NY-ESO-1 is one of the most immunogenic members of the cancer/testis antigen family and its levels can be increased after exposure to demethylating and deacetylating agents. This cytoplasmic antigen can serve as a potent target for cancer immunotherapy and yet has not been well studied in differentiated thyroid cancer cells. We studied the baseline expression of NY-ESO-1 messenger RNA and protein before and after exposure to 5-aza-2'-deoxycytidine (DAC) (72 hours) in a panel of thyroid cancer cell lines using quantitative polymerase chain reaction and Western blot. HLA-A2+, NY-ESO-1+ thyroid cell lines were then co-cultured with peripheral blood lymphocytes transduced with NY-ESO-1 specific T-cell receptor (TCR) and assayed for interferon-gamma and Granzyme-B release in the medium. SCID mice injected orthotopically with BCPAP cells were treated with DAC to evaluate for NY-ESO-1 gene expression in vivo. None of the thyroid cancer cell lines showed baseline expression of NY-ESO-1. Three cell lines, BCPAP, TPC-1, and 8505c, showed an increase in NY-ESO-1 gene expression with DAC treatment and were found to be HLA-A2 positive. DAC-treated target BCPAP and TPC-1 tumor cells with up-regulated NY-ESO-1 levels were able to mount an appropriate interferon-gamma and Granzyme-B response upon co-culture with the NY-ESO-1-TCR-transduced peripheral blood lymphocytes. In vivo DAC treatment was able to increase NY-ESO-1 expression in an orthotopic mouse model with BCPAP cells. Our data suggest that many differentiated thyroid cancer cells can be pressed to express immune antigens, which can then be utilized in TCR-based immunotherapeutic interventions.

  8. 76 FR 43259 - Foreign-Trade Zone 109-Watertown, NY, Application for Manufacturing Authority, North American...

    Science.gov (United States)

    2011-07-20

    ...) with textile backing material for the U.S. market and export. The manufacturing process under FTZ..., North American Tapes, LLC, (Textile Athletic Tape), Watertown, NY A request has been submitted to the... textile fabrics (duty rates: 10.5, 14.9%). The facility can produce up to 12.5 million square meters of...

  9. 78 FR 51061 - Special Anchorage Areas; Port of New York, NY

    Science.gov (United States)

    2013-08-20

    ... convenient access to public transportation, onshore shopping and amenities which facilitate interstate and... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 110 RIN 1625-AA01 Special Anchorage Areas; Port of New York, NY AGENCY...

  10. Modelling dune erosion, overwash and breaching at Fire Island (NY) during hurricane Sandy

    NARCIS (Netherlands)

    De Vet, P.L.M.; McCall, R.T.; Den Bieman, J.P.; Stive, M.J.F.; Van Ormondt, M.

    2015-01-01

    In 2012, Hurricane Sandy caused a breach at Fire Island (NY, USA), near Pelican Island. This paper aims at modelling dune erosion, overwash and breaching processes that occured during the hurricane event at this stretch of coast with the numerical model XBeach. By using the default settings, the ero

  11. 33 CFR 110.156 - Randall Bay, Freeport, Long Island, N.Y.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Randall Bay, Freeport, Long Island, N.Y. 110.156 Section 110.156 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND....Y. (a) The anchorage grounds. Southward of a line 312 feet south of and parallel to the south...

  12. 78 FR 23845 - Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY

    Science.gov (United States)

    2013-04-23

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY AGENCY... the Smith Point Bridge, mile 6.1, across Narrow Bay, between Smith Point and Fire Island, New York. The deviation is necessary to facilitate the Smith Point Triathlon. This deviation allows the...

  13. 76 FR 60733 - Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY

    Science.gov (United States)

    2011-09-30

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Narrow Bay, Smith Point, NY AGENCY... the Smith Point Bridge, 6.1, across Narrow Bay, between Smith Point and Fire Island, New York. The.... SUPPLEMENTARY INFORMATION: The Smith Point Bridge, across Narrow Bay, mile 6.1, between Smith Point and...

  14. Structural and magnetic properties of nanocomposite iron-containing SiCxNy films

    Science.gov (United States)

    Pushkarev, R. V.; Fainer, N. I.; Maurya, K. K.

    2017-02-01

    New ferromagnetic films with composition SiCxNyFez were synthesized using chemical vapor deposition technique. Films were deposited using ferrocene, 1,1,1,3,3,3-hexamethyldisilazane (HMDS) and hydrogen gaseous mixture. Chemical and phase composition of the films were studied by FTIR, Raman spectroscopy and X-ray diffraction with grazing incidence (GI-XRD). FTIR spectra analysis confirmed the existence of Si-C and Si-N bonds. Graphite inclusions and amorphous carbon were determined by Raman spectra analysis. The surface of the SiCxNyFez films studied by SEM is covered by nanocrystallites of iron oxide Fe3O4 phase. The main purpose of GI-XRD analysis is to describe the layered structure of the films in detail. It was shown by GI-XRD study, that phase composition of the SiCxNyFez films varies from iron oxide Fe3O4 to iron silicide Fe3Si and silicon carbide SiC with the deposition temperature growing. It was established, that SiCxNyFez films are perspective for application in the spintronic field.

  15. 76 FR 77175 - New York Fun Factory Fireworks Display, Western Long Island Sound; Mamaroneck, NY

    Science.gov (United States)

    2011-12-12

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 New York Fun Factory Fireworks Display, Western Long... Island Sound in the vicinity of Mamaroneck, NY in support of the New York Fun Factory Fireworks display... Guard to define regulatory safety zones. On May 10, 2012 New York Fun Factory Events is sponsoring...

  16. Den digitale patientmappe - et bud på en ny form for sundhedskommunikation i fysioterapeutisk praksis

    DEFF Research Database (Denmark)

    Duus, Lene

    2012-01-01

    Præsentation af et udviklingsprojekt, som jeg har deltaget i, hvor en ny form for sundhedskommunikation gennem en netbaseret digital patientmappe i fysioterapeutisk praksis har været afprøvet. Det overordnede mål for projektet var at bedre kommunikation, dokumentation og compliance for privatprak...

  17. 77 FR 74866 - Notice of Intent To Repatriate Cultural Items: New York State Museum, Albany, NY

    Science.gov (United States)

    2012-12-18

    ... belts. Seven of the wampum belts are on loan to the Seneca National Museum in Salamanca, NY, and one... Jemison on the Cattaraugus Reservation in New York. Mary Jemison was adopted by the Seneca as a child and chose to live her life as a Seneca. At the time of collection, the Five Nations Alliance Belt...

  18. 77 FR 30312 - Montezuma National Wildlife Refuge, Cayuga, Seneca, and Wayne Counties, NY; Draft Comprehensive...

    Science.gov (United States)

    2012-05-22

    ... notice in the Federal Register (75 FR 25286; May 7, 2010). Montezuma NWR was established in 1938 to... Fish and Wildlife Service Montezuma National Wildlife Refuge, Cayuga, Seneca, and Wayne Counties, NY... assessment (draft CCP/EA) for Montezuma National Wildlife Refuge (NWR), located in Cayuga, Seneca, and...

  19. 76 FR 3516 - Drawbridge Operation Regulations; Harlem River, New York City, NY

    Science.gov (United States)

    2011-01-20

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Harlem River, New York City, NY... of the 103rd Street (Wards Island) Bridge at mile 0.0, across the Harlem River, New York City, New... vessels, and commercial vessels of various sizes. The owner of the bridge, New York City Department of...

  20. 78 FR 35756 - Drawbridge Operation Regulations; Harlem River, New York City, NY

    Science.gov (United States)

    2013-06-14

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Harlem River, New York City, NY... of the Willis Avenue Bridge across the Harlem River, mile 1.5, at the Bronx, New York. The deviation... existing drawbridge operating regulations are found at 33 CFR 117.789(b)(2). The bridge owner, New York...

  1. 78 FR 23846 - Drawbridge Operation Regulations; Newtown Creek, New York City, NY

    Science.gov (United States)

    2013-04-23

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Newtown Creek, New York City, NY... of the Greenpoint Avenue Bridge across Newtown Creek, mile 1.3, at New York City, New York. The... are found at 33 CFR 117.801(g). The bridge owner, New York City Department of Transportation...

  2. 75 FR 36110 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Science.gov (United States)

    2010-06-24

    ... National Park Service Notice of Inventory Completion: New York University College of Dentistry, New York... Dentistry, New York, NY. The human remains were removed from Broward and Levy Counties, FL, and an unknown... assessment of the human remains was made by the New York University College of Dentistry professional...

  3. 75 FR 33327 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Science.gov (United States)

    2010-06-11

    ... National Park Service Notice of Inventory Completion: New York University College of Dentistry, New York... Dentistry, New York, NY. The human remains were removed from the Allred Bluff and Salts Bluff Rockshelters... assessment of the human remains was made by the New York University College of Dentistry professional...

  4. 75 FR 33329 - Notice of Inventory Completion: New York University College of Dentistry, New York, NY

    Science.gov (United States)

    2010-06-11

    ... National Park Service Notice of Inventory Completion: New York University College of Dentistry, New York... Dentistry, New York, NY. The human remains were removed from Lovelock Cave, Churchill County, NV. This... remains was made by the New York University College of Dentistry professional staff in consultation...

  5. 78 FR 36301 - CSX Transportation, Inc.-Discontinuance of Service Exemption-in Oswego County, NY

    Science.gov (United States)

    2013-06-17

    ... 0.85-mile rail line on CSXT's Northern Region, Albany Division, Fulton Subdivision, between milepost QMF 37.10 at the connection with CSXT's main line and milepost QMF 37.95 at the end of the track, in Oswego County, NY (the Line). The Line traverses United States Postal Service Zip Code 13126,...

  6. 78 FR 74009 - Safety Zone; Nike Fireworks, Upper New York Bay, Ellis Island, NY

    Science.gov (United States)

    2013-12-10

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 Safety Zone; Nike Fireworks, Upper New York Bay, Ellis Island, NY..., 2011 (76 FR 69614). ] Table 1 1. Nike Fireworks Ellis Launch site: A barge located Island Safety...

  7. 78 FR 71546 - Safety Zone; Belt Parkway Bridge Construction, Gerritsen Inlet, Brooklyn, NY

    Science.gov (United States)

    2013-11-29

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Belt Parkway Bridge Construction, Gerritsen Inlet, Brooklyn, NY AGENCY: Coast Guard, DHS. ACTION: Notice of proposed rulemaking. SUMMARY: The Coast... Belt Parkway Bridge. This proposed rule would allow the Coast Guard to prohibit all vessel traffic...

  8. 76 FR 4529 - Safety Zone; Underwater Hazard, Gravesend Bay, Brooklyn, NY

    Science.gov (United States)

    2011-01-26

    ... 02'14'' W (NAD 83), approximately 70 yards southeast of the Verrazano Bridge Brooklyn tower. All... Bridge, Brooklyn, NY. This safety zone will not have a significant economic impact on a substantial...), approximately 70-yards southeast of the Verrazano Bridge Brooklyn tower. (b) Effective period. This regulation...

  9. 76 FR 34865 - Safety Zone; Rochester Harbor Festival, Genesee River, Rochester, NY

    Science.gov (United States)

    2011-06-15

    ... impracticable and contrary to the public interest. Background and Purpose The Rochester Harbor Festival is an... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Rochester Harbor Festival, Genesee River... establishing a temporary safety zone on the Genesee River, Rochester, NY for the Rochester Harbor Festival...

  10. 77 FR 52058 - Notice of Inventory Completion: American Museum of Natural History, New York, NY; Correction

    Science.gov (United States)

    2012-08-28

    ... National Park Service Notice of Inventory Completion: American Museum of Natural History, New York, NY... completion of an inventory of human remains in the possession of the American Museum of Natural History, New... following sentence: Officials of the American Museum of Natural History have determined that, pursuant to...

  11. 76 FR 43712 - Notice of Inventory Completion: American Museum of Natural History, New York, NY

    Science.gov (United States)

    2011-07-21

    ... National Park Service Notice of Inventory Completion: American Museum of Natural History, New York, NY AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The American Museum of Natural History... human remains may contact the American Museum of Natural History. Repatriation of the human remains...

  12. NY-ESO-1 expression in DCIS: A new predictor of good prognosis.

    Science.gov (United States)

    Coombes, R Charles; Caballero, Otavia L; Shousha, Sami; Ghaem-Maghami, Sadaf; Woodley-Barker, Laura; Wilhelm-Benartzi, Charlotte S; Neville, A Munro

    2017-03-01

    At present, it is difficult to predict which patients with ductal carcinoma-in-situ (DCIS) will subsequently develop frank invasive breast cancer (IDC). A recent survey by our group has shown that NY-ESO-1 and MAGEA are both expressed in DCIS. This study was aimed at determining whether expression of these antigens was related to the later development of IDC. 14 of 42 (33%) of patients developed invasive breast cancer during the follow up period. Only one of those DCIS cases that relapsed was positive for NYESO-1 at diagnosis. In contrast, DCIS samples of 15 of the 28 (54%) of those patients who remained disease-free expressed NY-ESO-1. (Permutation chi square p=0.0033). We identified 42 patients with DCIS, and followed them up for more than 10 years. NY-ESO-1 and MAGEA were demonstrated by immunostaining as were CD8+ infiltrates on all sections together with the conventional markers, ER, PR, and HER2. Expression of NY-ESO-1 may predict those patients who will not subsequently develop invasive breast cancer and could therefore potentially be helpful in defining prognosis in patients with DCIS.

  13. 77 FR 46349 - Safety Zone; DeStefano Wedding Fireworks Display, Patchogue Bay, Patchogue, NY

    Science.gov (United States)

    2012-08-03

    ... significant environmental impact from this proposed rule. List of Subjects in 33 CFR Part 165 Harbors, Marine... Bay'' restaurant in Patchogue, NY. This rule will be effective from 8:30 p.m. on November 3, 2012... Broadcast Notice to Mariners. 2. Impact on Small Entities Under the Regulatory Flexibility Act (5 U.S.C....

  14. 75 FR 13454 - Special Local Regulation, Fran Schnarr Open Water Championships, Huntington Bay, NY

    Science.gov (United States)

    2010-03-22

    ... Federal Register (73 FR 3316). Public Meeting We do not plan to hold a public meeting. But you may submit...-2009-0520) in the Federal Register (74 FR 51243). No comments or requests for meetings were received... Championships, Huntington Bay, NY AGENCY: Coast Guard, DHS. ACTION: Supplemental Notice of proposed...

  15. NY Serpentis: SU UMa-type nova in the period gap with diversity of normal outbursts

    Science.gov (United States)

    Pavlenko, Elena P.; Kato, Taichi; Antonyuk, Oksana I.; Ohshima, Tomohito; Hambsch, Franz-Josef; Antonyuk, Kirill A.; Sosnovskij, Aleksei A.; Baklanov, Alex V.; Shugarov, Sergey Yu.; Pit, Nikolaj V.; Nakata, Chikako; Masi, Gianluca; Nakajima, Kazuhiro; Maehara, Hiroyuki; Dubovsky, Pavol A.; Kudzej, Igor; Andreev, Maksim V.; Kuznyetsova, Yuliana G.; Vasiliskov, Kirill A.

    2014-12-01

    We present a photometric study of NY Ser, an in-the-gap SU UMa-type nova, in 2002 and 2013. We determined the duration of its superoutburst and the mean period of its superhump are 18 d and 0.10458 d, respectively. We detected in 2013 that NY Ser showed two distinct states separated by a superoutburst. A state of rather infrequent normal outbursts lasted at least 44 d before the superoutburst, and a state of frequent outbursts started immediately after the superoutburst and lasted at least 34 d. Unlike a typical SU UMa star with a bimodal distribution of outburst duration, NY Ser displayed a diversity of normal outbursts. In the state of infrequent outbursts, we detected a wide ˜ 12 d outburst accompanied by 0.098 d orbital modulation but without superhumps ever established in NY Ser. We classified this as a "wide normal outburst." The orbital period was dominant both in quiescence and during normal outbursts in this state. In the state of the most frequent normal outbursts, the 0.10465 d positive superhump period was dominant and coexisted with the orbital modulation. In 2002 we detected the normal outburst of "intermediate" 5-6 d duration that was also accompanied by orbital modulations.

  16. 75 FR 15739 - International Business Machines Corporation: Armonk, NY; Notice of Termination of Investigation

    Science.gov (United States)

    2010-03-30

    ... Employment and Training Administration International Business Machines Corporation: Armonk, NY; Notice of... was initiated in response to a petition filed on May 21, 2009 on behalf of workers of International Business Machines Corporation, Armonk, New York. The petitioning group of workers is covered by...

  17. Nematodes from rocky grassland in Börzsöny Mountains, Hungary

    OpenAIRE

    Kiss, M

    2011-01-01

    This paper reports on investigations carried out on soil inhabiting nematodes living in andesitic rock regions ofBörzsöny Mountains. Seventeen species are listed. Heterodorus arcuatus (Thorne, 1939), new to the fauna of Hungary, isdescribed and illustrated in detail.

  18. Nematodes from rocky grassland in Börzsöny Mountains, Hungary

    Directory of Open Access Journals (Sweden)

    Kiss, M.

    2011-06-01

    Full Text Available This paper reports on investigations carried out on soil inhabiting nematodes living in andesitic rock regions ofBörzsöny Mountains. Seventeen species are listed. Heterodorus arcuatus (Thorne, 1939, new to the fauna of Hungary, isdescribed and illustrated in detail.

  19. 77 FR 41271 - Safety Zone; Newburgh to Beacon Swim, Newburgh, Hudson River, NY

    Science.gov (United States)

    2012-07-13

    ... Acronyms DHS Department of Homeland Security FR Federal Register CFR Code of Federal Regulations NPRM... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Newburgh to Beacon Swim, Newburgh, Hudson... Newburgh, NY for the annual Newburgh Beacon Swim event. This temporary safety zone is necessary to...

  20. 77 FR 834 - Noise Exposure Map Update for Albany International Airport, Albany, NY

    Science.gov (United States)

    2012-01-06

    ... Federal Aviation Administration Noise Exposure Map Update for Albany International Airport, Albany, NY... Administration (FAA) announces its determination that the updated noise exposure maps submitted by the Albany.... 47501 et. seq (Aviation Safety and Noise Abatement Act) and 14 CFR part 150 are in compliance...

  1. 77 FR 51614 - CSX Transportation, Inc.-Abandonment Exemption-in Niagara County, NY

    Science.gov (United States)

    2012-08-24

    ... Abandonments to abandon a 0.1-mile rail line on its Northern Region, Albany Division, Niagara Subdivision, between milepost QDN 28.0 near North Avenue to the end of the track at milepost QDN 28.1, in Niagara Falls... Surface Transportation Board CSX Transportation, Inc.--Abandonment Exemption--in Niagara County, NY...

  2. 77 FR 41048 - Safety Zone; Hudson Valley Triathlon, Ulster Landing, Hudson River, NY

    Science.gov (United States)

    2012-07-12

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Hudson Valley Triathlon, Ulster Landing... Landing, NY for the 16th Annual Hudson Valley Triathlon swim event. This temporary safety zone is.... Regulatory History and Information The Hudson Valley Triathlon swim is an annual recurring event that has...

  3. 75 FR 11937 - Eagle Sportswear, Inc.; New York, NY; Notice of Termination of Investigation

    Science.gov (United States)

    2010-03-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF LABOR Employment and Training Administration Eagle Sportswear, Inc.; New York, NY; Notice of Termination of... response to a petition filed on December 4, 2009 by a company official on behalf of workers of...

  4. Influence of residual stress on the phase equilibrium of Ti(CxNy) thin films

    Institute of Scientific and Technical Information of China (English)

    Ying Jin; Changrong Li; Xiaoyan Ma; Zhenmin Du; Weijing Zhang; J.-C. Tedenac

    2003-01-01

    In order to provide a theoretic basis for the research of Ti(CxNy) thin films, the thermodynamic database of Ti-C-N ternary system is established and the phase diagram sections are calculated. In addition to the assessed thermodynamic properties of Ti-C-N system, the influence of the residual strain energy of Ti(CxNy) thin films on the phase equilibria is analyzed. The classical formula for calculating the elastic strain energy is expressed into a Redlich-Kister form in order to perform the thermodynamic and equilibrium calculations using the Thermo-Calc software. Isothermal sections at 900 and 1100 K are calculated with this database and compared with those calculated without considering the residual stress. As a result, with the addition of strain energy δ-fcc Ti(CxNy) phase area shrinks. It is therefore concluded that with the influence of the residual stress in Ti(CxNy) thin solid film, the precipitation of pure δ film requires more precise control of composition.

  5. Modelling dune erosion, overwash and breaching at Fire Island (NY) during hurricane Sandy

    NARCIS (Netherlands)

    De Vet, P.L.M.; McCall, R.T.; Den Bieman, J.P.; Stive, M.J.F.; Van Ormondt, M.

    2015-01-01

    In 2012, Hurricane Sandy caused a breach at Fire Island (NY, USA), near Pelican Island. This paper aims at modelling dune erosion, overwash and breaching processes that occured during the hurricane event at this stretch of coast with the numerical model XBeach. By using the default settings, the

  6. 77 FR 38492 - Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY

    Science.gov (United States)

    2012-06-28

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY... temporary safety zone on Lake Ontario, Olcott, New York. This safety zone is intended to restrict vessels from a portion of Lake Ontario during the Olcott fireworks on July 3, 2012. The safety zone...

  7. Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer.

    Directory of Open Access Journals (Sweden)

    Katy Milne

    Full Text Available BACKGROUND: Tumor-infiltrating CD8+ T cells are correlated with prolonged progression-free and overall survival in epithelial ovarian cancer (EOC. A significant fraction of EOC patients mount autoantibody responses to various tumor antigens, however the relationship between autoantibodies and tumor-infiltrating T cells has not been investigated in EOC or any other human cancer. We hypothesized that autoantibody and T cell responses may be correlated in EOC and directed toward the same antigens. METHODOLOGY AND PRINCIPAL FINDINGS: We obtained matched serum and tumor tissue from 35 patients with high-grade serous ovarian cancer. Serum samples were assessed by ELISA for autoantibodies to the common tumor antigen NY-ESO-1. Tumor tissue was examined by immunohistochemistry for expression of NY-ESO-1, various T cell markers (CD3, CD4, CD8, CD25, FoxP3, TIA-1 and Granzyme B and other immunological markers (CD20, MHC class I and MHC class II. Lymphocytic infiltrates varied widely among tumors and included cells positive for CD3, CD8, TIA-1, CD25, FoxP3 and CD4. Twenty-six percent (9/35 of patients demonstrated serum IgG autoantibodies to NY-ESO-1, which were positively correlated with expression of NY-ESO-1 antigen by tumor cells (r = 0.57, p = 0.0004. Autoantibodies to NY-ESO-1 were associated with increased tumor-infiltrating CD8+, CD4+ and FoxP3+ cells. In an individual HLA-A2+ patient with autoantibodies to NY-ESO-1, CD8+ T cells isolated from solid tumor and ascites were reactive to NY-ESO-1 by IFN-gamma ELISPOT and MHC class I pentamer staining. CONCLUSION AND SIGNIFICANCE: We demonstrate that tumor-specific autoantibodies and tumor-infiltrating T cells are correlated in human cancer and can be directed against the same target antigen. This implies that autoantibodies may collaborate with tumor-infiltrating T cells to influence clinical outcomes in EOC. Furthermore, serological screening methods may prove useful for identifying clinically relevant

  8. The tubulin-bound conformation of paclitaxel: T-taxol vs "PTX-NY".

    Science.gov (United States)

    Yang, Yutao; Alcaraz, Ana A; Snyder, James P

    2009-03-27

    Nearly 35 years after its discovery and 11 years after FDA approval of paclitaxel (PTX) as a breakthrough anticancer drug, the 3-D structure of the agent bound to its beta-tubulin target was proposed to be T-Taxol. The latter bioactive form has recently been challenged by the Ojima group with a structure, "PTX-NY" ("REDOR Taxol"), in which the C-13 side chain is proposed to adopt a different conformation and an alternative hydrogen-bonding pattern in the tubulin binding site. Previously, the two conformers were compared to show that only T-Taxol fits the PTX-derived electron crystallographic density. That work has been extended by molecular mechanics and quantum chemical methods to reveal that the PTX-NY conformation is relatively less stable, on average, by 10-11 kcal/mol. In agreement with NMR studies, an 11 ns molecular dynamics treatment for PTX in an explicit water pool locates T-Taxol along the trajectory, but not PTX-NY. Docking of various PTX conformers into the electron crystallographic binding site of tubulin demonstrates that PTX-NY cannot be accommodated unless the pocket is reorganized in violation of the experimental constraints. Finally, analysis of the structures of T-Taxol and PTX-NY for their capacity to predict the existence of superpotent PTX analogues discloses that only the former forecasts such analogues, as now established by the T-Taxol-inspired synthesis of bridged taxanes. In sum, all empirical criteria support T-Taxol as the bound conformation of PTX on beta-tubulin in microtubules.

  9. NY-BR-1 protein expression in breast carcinoma: a mammary gland differentiation antigen as target for cancer immunotherapy.

    Science.gov (United States)

    Theurillat, Jean-Philippe; Zürrer-Härdi, Ursina; Varga, Zsuzsanna; Storz, Martina; Probst-Hensch, Nicole M; Seifert, Burkhardt; Fehr, Mathias K; Fink, Daniel; Ferrone, Soldano; Pestalozzi, Bernhard; Jungbluth, Achim A; Chen, Yao-Tseng; Jäger, Dirk; Knuth, Alexander; Moch, Holger

    2007-11-01

    NY-BR-1 is a recently identified differentiation antigen of the mammary gland. To use NY-BR-1 for T-cell-based immunotherapy, analysis of its co-expression with HLA class I antigens is required. In the present tissue microarray study, primary breast cancers (n = 1,444), recurrences (n = 88), lymph node (n = 525) and distant metastases (n = 91) were studied for NY-BR-1 expression using a novel monoclonal antibody. NY-BR-1 expression was compared with prognosis, estrogen receptor, HER2-status, EGFR and HLA class I antigen expression. NY-BR-1 was more frequently expressed in grade 1 (82%) than in grade 2 (69%) and grade 3 (46%) carcinomas (P < 0.0001). Moreover, NY-BR-1 expression correlated directly with estrogen receptor expression (P < 0.0001) and inversely correlated with HER2-status and EGFR expression (P < 0.0001 for both). Considering high expression level of co-expression, 198/1,321 (15%) primary breast carcinomas and 4/65 (6%) distant metastases expressed NY-BR-1 and HLA class I, suggesting that active immunotherapy can be applied to about 10% of breast cancer patients. Survival analysis showed an association of NY-BR-1 expression with better patient outcome (P = 0.015). No difference between NY-BR-1 expression of primary tumors and metastases could be found, indicating that the presence of NY-BR-1 in metastases can be deduced from their corresponding primary. Forty-three paired biopsies taken from patients before and after chemotherapy suggest that NY-BR-1 expression is not influenced by preceding chemotherapy (kappa = 0.89, P < 0.0001). In summary, the co-expression of NY-BR-1 with HLA class I antigens and its expression in metastases without modification by chemotherapy suggest that NY-BR-1 targeted immunotherapy represents a viable strategy in addition to other targeted cancer drug therapies of breast cancer.

  10. 重组腺病毒载体pAd-NY-ESO-1-EGFP的构建及鉴定%Construction and Identification of Recombinant Adenovirns Vector Containing NY-ESO-1 Gene

    Institute of Scientific and Technical Information of China (English)

    张又莉; 徐少勇; 王家宁; 黄永章; 王斌; 宋军; 郭凌郧

    2008-01-01

    目的:利用细菌内同源重组法构建含人肿瘤特异性抗原的腺病毒质粒.方法:设计NY-ESO-1 cDNA扩增引物,从pETl5b-NY-ESO-1质粒中扩增NY-ESO-1的DNA序列,与pShuttle-IRES-hrGFP-2进行连接,PCR和EcoRV酶切鉴定重组质粒pShuttle-NY-ESO-1-EGFP,经Pme I酶切线性化后,转化含pAdeasy-1的超感受态BJ5183大肠杆菌,细菌内同源重组法构建腺病毒质粒pAd-NY-ESO-1-EGFP;质粒经Pac I酶切鉴定,DNA测序.结果:线性化的pShuttle-NY-ESO-1-EGFP转化含pAdeasy-1的超感受态BJ5183大肠杆菌,重组质粒经酶切获得一大于23 kb的大片段和4.5kb的片段,PCR反应扩增出了340 bp的片段.结论:用细菌内同源重组法成功地构建了含NY-ESO-1的重组腺病毒质粒,为进行表达NY-ESO-1的重组腺病毒的制备及肿瘤特异性抗原NY-ESO-1的核酸疫苗在肿瘤方面的研究奠定了基础.

  11. Polymeric Structure and Host Toll-like Receptor 4 Dictate Immunogenicity of NY-ESO-1 Antigen in Vivo*

    Science.gov (United States)

    Liu, Yanan; Tian, Xiaoli; Leitner, Wolfgang W.; Aldridge, Michael E.; Zheng, Junying; Yu, Zhiya; Restifo, Nicholas P.; Weiss, Richard; Scheiblhofer, Sandra; Xie, Chong; Sun, Ren; Cheng, Genhong; Zeng, Gang

    2011-01-01

    In search of intrinsic factors that contribute to the distinctively strong immunogenicity of a non-mutated cancer/testis antigen, we found that NY-ESO-1 forms polymeric structures through disulfide bonds. NY-ESO-1 binding to immature dendritic cells was dependent on its polymeric structure and involved Toll-like receptor-4 (TLR4) on the surface of immature dendritic cells in mouse and human. Gene gun-delivered plasmid encoding the wild-type NY-ESO-1 readily induced T cell-dependent antibody (Ab) responses in wild-type C57BL/10 mice but not TLR4-knock-out C57BL/10ScNJ mice. Disrupting polymeric structures of NY-ESO-1 by cysteine-to-serine (Cys-to-Ser) substitutions lead to diminished immunogenicity and altered TLR4-dependence in the induced Ab response. To demonstrate its adjuvant effect, NY-ESO-1 was fused with a major mugwort pollen allergen Art v 1 and a tumor-associated antigen, carbonic anhydrase 9. Plasmid DNA vaccines encoding the fusion genes generated robust immune responses against otherwise non-immunogenic targets in mice. Polymeric structure and TLR4 may play important roles in rendering NY-ESO-1 immunogenic and thus serve as a potent molecular adjuvant. NY-ESO-1 thus represents the first example of a cancer/testis antigen that is a also damage-associated molecular pattern. PMID:21900253

  12. Polymeric structure and host Toll-like receptor 4 dictate immunogenicity of NY-ESO-1 antigen in vivo.

    Science.gov (United States)

    Liu, Yanan; Tian, Xiaoli; Leitner, Wolfgang W; Aldridge, Michael E; Zheng, Junying; Yu, Zhiya; Restifo, Nicholas P; Weiss, Richard; Scheiblhofer, Sandra; Xie, Chong; Sun, Ren; Cheng, Genhong; Zeng, Gang

    2011-10-28

    In search of intrinsic factors that contribute to the distinctively strong immunogenicity of a non-mutated cancer/testis antigen, we found that NY-ESO-1 forms polymeric structures through disulfide bonds. NY-ESO-1 binding to immature dendritic cells was dependent on its polymeric structure and involved Toll-like receptor-4 (TLR4) on the surface of immature dendritic cells in mouse and human. Gene gun-delivered plasmid encoding the wild-type NY-ESO-1 readily induced T cell-dependent antibody (Ab) responses in wild-type C57BL/10 mice but not TLR4-knock-out C57BL/10ScNJ mice. Disrupting polymeric structures of NY-ESO-1 by cysteine-to-serine (Cys-to-Ser) substitutions lead to diminished immunogenicity and altered TLR4-dependence in the induced Ab response. To demonstrate its adjuvant effect, NY-ESO-1 was fused with a major mugwort pollen allergen Art v 1 and a tumor-associated antigen, carbonic anhydrase 9. Plasmid DNA vaccines encoding the fusion genes generated robust immune responses against otherwise non-immunogenic targets in mice. Polymeric structure and TLR4 may play important roles in rendering NY-ESO-1 immunogenic and thus serve as a potent molecular adjuvant. NY-ESO-1 thus represents the first example of a cancer/testis antigen that is a also damage-associated molecular pattern.

  13. Preclinical safety pharmacology study of a novel protein-based cancer vaccine CHP-NY-ESO-1.

    Science.gov (United States)

    Harada, Naozumi; Hoshiai, Kiyotaka; Takahashi, Yoshiyasu; Sakaguchi, Yasue; Kuno, Takayoshi; Hishida, Tadashi; Shiku, Hiroshi

    2008-05-23

    CHP-NY-ESO-1 is a novel therapeutic cancer vaccine consisting of a recombinant protein of cancer antigen NY-ESO-1 and a polysaccharide-based delivery system, cholesteryl pullulan. A pilot clinical study of CHP-NY-ESO-1 in cancer patients was previously conducted, and the adverse events related to this drug were observed to be limited to skin reactions at injection sites. To further establish the safety of CHP-NY-ESO-1, we studied the effects of its subcutaneous injection on vital functions such as the central nervous system, cardiovascular system and respiratory system using preclinical animal models. The effects of CHP-NY-ESO-1 on the cardiovascular system were investigated in dogs using a telemetry system for blood pressure and heart rate and the Holter monitoring for ambulatory electrocardiograms. No drug-related changes were observed in these parameters. The effect of CHP-NY-ESO-1 on the hERG-dependent potassium currents was also examined using in vitro cultured cell system, and no inhibition of hERG currents was observed. The effects of CHP-NY-ESO-1 on the central nervous system were examined in rats using functional observational battery method, and no drug-related changes were observed in home cage observations, open field observations, hand held observations, and perception and motor function observations. The effect of CHP-NY-ESO-1 on the respiratory system was investigated in rats by measuring tidal volume, minute volume and respiratory rate using whole-body plethysmograph method, and no significant changes were found in these parameters. These results indicate that CHP-NY-ESO-1 would not have any pharmacological effects on vital functions and support the safety of this cancer vaccine for clinical use.

  14. Enhancing whole-tumor cell vaccination by engaging innate immune system through NY-ESO-1/dendritic cell interactions.

    Science.gov (United States)

    Xu, Le; Zheng, Junying; Nguyen, David H; Luong, Quang T; Zeng, Gang

    2013-10-01

    NY-ESO-1 is a cancer/germline antigen (Ag) with distinctively strong immunogenicity. We have previously demonstrated that NY-ESO-1 serves as an endogenous adjuvant by engaging dendritic cell (DC)-surface receptors of calreticulin (CRT) and toll-like receptor (TLR) 4. In the present study, NY-ESO-1 was investigated for its immunomodulatory roles as a molecular adjuvant in whole-tumor cell vaccines using the Renca kidney cancer model. Renca cells were genetically engineered to express NY-ESO-1 on the cell surface to enhance direct interactions with DC. The effect of ectopic cell-surface expression of NY-ESO-1 was investigated on tumor immunogenicity, DC activation, cytotoxic T lymphocytes against model tumor-associated Ags, and the effectiveness of the modified tumor cells as a therapeutic whole-cell vaccine. Cell-surface expression of NY-ESO-1 was able to reduce the tumor growth of Renca cells in BALB/c mice, although the modification did not alter cell proliferation rate in vitro. Directly engaging the innate immune system through NY-ESO-1 facilitated the interaction of tumor cells with DC, leading to enhanced DC activation and subsequent tumor-specific T-cell priming. When used as a therapeutic whole-cell vaccine, Renca cells with NY-ESO-1 on the surface mediated stronger inhibitory effects on tumor growth and metastasis compared with parental Renca or Renca cells expressing a control protein GFP on the surface. Augmented antitumor efficacy correlated with increased CD8 T-cell infiltration into tumors and decreased myeloid-derived suppressor cells and regulatory T cells in the spleen. As a cancer/germline Ag and as an immunomodulatory adjuvant through engaging innate immune receptors, NY-ESO-1 offers a unique opportunity for improved whole-tumor cell vaccinations upon the classic GM-CSF-engineered cell vaccines.

  15. NY-ESO-1 is a ubiquitous immunotherapeutic target antigen for patients with myxoid/round cell liposarcoma.

    Science.gov (United States)

    Pollack, Seth M; Jungbluth, Achim A; Hoch, Benjamin L; Farrar, Erik A; Bleakley, Marie; Schneider, David J; Loggers, Elizabeth T; Rodler, Eve; Eary, Janet F; Conrad, Ernest U; Jones, Robin L; Yee, Cassian

    2012-09-15

    Myxoid/round cell liposarcoma (MRCL) is the second most common liposarcoma subtype, accounting for >33% of liposarcomas and approximately 10% of all soft tissue sarcomas. Although MRCL is a chemosensitive subtype, patients with metastatic disease have a poor outcome. NY-ESO-1 is a cancer-testis antigen (also known as cancer germ cell antigen) that has been successfully targeted in vaccine trials and in adoptive T-cell therapy trials for the treatment of several solid tumors. The authors investigated the feasibility of targeting NY-ESO-1 in patients with MRCL by evaluating the prevalence of NY-ESO-1 expression in tumors using immunohistochemistry and quantitative reverse transcriptase-polymerase chain reaction analysis. NY-ESO-1-specific tumor recognition by NY-ESO-1-specific T-cells also was analyzed using a chromium release assay. A search of the University of Washington Sarcoma Tissue Bank identified paraffin-embedded tumor samples from 25 patients with MRCL. NY-ESO-1 expression was observed in every MRCL tumor assessed (100%); in 18 tumors (72%), staining was homogenous. In all but 2 tumors, staining was sufficiently robust (2+) that such patients would be eligible for clinical trials of NY-ESO-1-directed therapy. By using NY-ESO-1 specific, CD8-positive T-cells, the in vitro sensitivity of myxoid liposarcoma cell lines to antigen-specific lysis was demonstrated. The current results establish NY-ESO-1 as an important target antigen for the treatment of patients with MRCL. Copyright © 2012 American Cancer Society.

  16. Effector memory and central memory NY-ESO-1-specific re-directed T cells for treatment of multiple myeloma.

    Science.gov (United States)

    Schuberth, P C; Jakka, G; Jensen, S M; Wadle, A; Gautschi, F; Haley, D; Haile, S; Mischo, A; Held, G; Thiel, M; Tinguely, M; Bifulco, C B; Fox, B A; Renner, C; Petrausch, U

    2013-04-01

    The cancer-testis antigen NY-ESO-1 is a potential target antigen for immune therapy expressed in a subset of patients with multiple myeloma. We generated chimeric antigen receptors (CARs) recognizing the immunodominant NY-ESO-1 peptide 157-165 in the context of HLA-A*02:01 to re-direct autologous CD8(+) T cells towards NY-ESO-1(+) myeloma cells. These re-directed T cells specifically lysed NY-ESO-1(157-165)/HLA-A*02:01-positive cells and secreted IFNγ. A total of 40% of CCR7(-) re-directed T cells had an effector memory phenotype and 5% a central memory phenotype. Based on CCR7 cell sorting, effector and memory CAR-positive T cells were separated and CCR7(+) memory cells demonstrated after antigen-specific re-stimulation downregulation of CCR7 as sign of differentiation towards effector cells accompanied by an increased secretion of memory signature cytokines such as IL-2. To evaluate NY-ESO-1 as potential target antigen, we screened 78 bone marrow biopsies of multiple myeloma patients where NY-ESO-1 protein was found to be expressed by immunohistochemistry in 9.7% of samples. Adoptively transferred NY-ESO-1-specific re-directed T cells protected mice against challenge with endogenously NY-ESO-1-positive myeloma cells in a xenograft model. In conclusion, re-directed effector- and central memory T cells specifically recognized NY-ESO-1(157-165)/ HLA-A*02:01-positive cells resulting in antigen-specific functionality in vitro and in vivo.

  17. Distinct expression patterns of the immunogenic differentiation antigen NY-BR-1 in normal breast, testis and their malignant counterparts.

    Science.gov (United States)

    Theurillat, Jean-Philippe; Zürrer-Härdi, Ursina; Varga, Zsuzsanna; Barghorn, André; Saller, Elisabeth; Frei, Claudia; Storz, Martina; Behnke, Silvia; Seifert, Burkhardt; Fehr, Mathias; Fink, Daniel; Rageth, Christoph; Linsenmeier, Claudia; Pestalozzi, Bernhard; Chen, Yao-Tseng; Knuth, Alexander; Jäger, Dirk; Moch, Holger

    2008-04-01

    NY-BR-1 is a differentiation antigen and a potential target for cancer immunotherapy. Its mRNA expression is restricted to breast, testis, prostate and breast cancer by RT-PCR. In this study, we correlated NY-BR-1 protein and mRNA expression on tissue microarrays of mammary, prostatic and testicular malignancies using immunohistochemistry and in situ hybridization with probes for exon 4-7 and 30-33. NY-BR-1 mRNA was confined to primary spermatocytes, suggesting a role in spermatogenesis. Exon 4-7 and 30-33 were equally expressed this cell type. However, NY-BR-1 was absent in all germ cell tumours analyzed (n = 475) and present in one of 56 (2%) prostate carcinomas. In breast, NY-BR-1 mRNA expression was detected in 307 of 442 (70%) primary carcinomas, with strong correlation to its protein expression (p < 0.0001). mRNA expression was significantly stronger and more frequently detected by the exon 30-33 probe than by the exon 4-7 probe (70% vs. 35%, p < 0.0001), indicating the presence of alternative splice variants that lack 5-prime sequences. A similar restricted mRNA pattern was also observed in the normal breast epithelium. NY-BR-1 protein and mRNA correlated significantly with estrogen receptor alpha (ER alpha) protein expression (p < 0.0001), with stronger association to NY-BR-1 mRNA than protein (odds ratio 7.7 compared to 4.6). We identified 4 estrogen response elements (ERE)-like sequences nearby the promoter region, suggesting that NY-BR-1 transcription might be controlled by ER alpha. Accordingly, analysis of matching pairs of primary tumors with their recurrences showed a marked decrease of NY-BR-1 expression in recurrences after tamoxifen treatment (p < 0.0001).

  18. MAGE-A3/4 and NY-ESO-1 antigens expression in metastatic esophageal squamous cell carcinoma.

    Science.gov (United States)

    Bujas, T; Marusic, Z; Peric Balja, M; Mijic, A; Kruslin, B; Tomas, D

    2011-03-21

    In the present study we analyzed immunohistochemical expression of MAGE-A 3/4 and NY-ESO-1 in 55 samples of esophageal squamous cell carcinomas (ESCC) and their respective lymph node metastases. To our knowledge this is the first study to assess and compare the expression of these antigens in ESCC lymph node metastases. Fifty (90.9%) primary ESCC were positive for MAGE-A 3/4 and 53 (96.6%) were positive for NY-ESO-1. MAGE-A 3/4 was expressed in all lymph node metastases and the intensity of expression was high in a majority of cases. NY-ESO-1 was negative in 2 (7.1%) lymph nodes metastases, while the reaction was predominantly moderate in the positive group. In primary tumors MAGE-A 3/4 showed a significantly higher intensity of expression compared to NY-ESO-1 (P=0.047), while in lymph node metastases the intensity of expression was not significantly different (P=0.387). Primary tumors with and without lymph node metastases showed no significant differences in MAGE-A 3/4 (P=0.672) and NY-ESO-1 (P=0.444) expression. Intensity of MAGE-A 3/4 (P=0.461) and NY-ESO-1 (P=0.414) expression in primary tumors was not significantly different compared to the expression in their respective lymph nodes metastases. Expression of MAGE-A 3/4 in primary tumors showed significant positive correlation with primary tumor expression of NY-ESO-1 (P=0.021) but no significant correlation with the expression of MAGE-A 3/4 in lymph node metastases (P=0.056). Expression of NY-ESO-1 in primary tumors showed significant positive correlation with the expression of NY-ESO-1 in lymph node metastases (P=0.001) and significant negative correlation with patients’ age (P<0.001). Expression of MAGE-A 3/4 and NY-ESO-1 in primary tumors and lymph node metastases showed no significant correlation with prognostic parameters such as tumor grade and TNM stage (P>0.05). We have shown different levels of MAGE-A 3/4 and NY-ESO-1 expression in almost all specimens of primary tumor and lymph node metastases

  19. Expression and clinical significance of NY-ESO-1 protein in human laryngeal squamous carcinoma%NY-ESO-1蛋白在喉鳞状细胞癌中表达的意义

    Institute of Scientific and Technical Information of China (English)

    田俊; 王斌全; 李建民; 皇甫辉; 温树信; 张艳廷

    2010-01-01

    目的 检测NY-ESO-1蛋白在喉鳞癌中的表达情况,探讨其作为喉癌免疫治疗靶标的实验依据及其与喉癌生物学行为的关系.方法 免疫组织化学PV-9000两步法检测69例喉鳞癌患者原发灶及其常规病理报告为阳性的121个颈淋巴结中NY-ESO-1的表达,同时采用Western blotting方法检测其在喉鳞癌标本的癌中心区、癌周0.5、1.0 cm及9例全喉切除术患者的喉正常黏膜组织(对照)中的表达.结果 69例喉鳞癌组织中,NY-ESO-1蛋白表达阳性率为43.48%(30/69),在癌中心区、癌周0.5、1.0cm中的表达逐渐下降(P<0.05),NY-ESO-1在对照组喉正常黏膜组织中无表达.NY-ESO-1蛋白在不同T分级、病理分级和不同颈淋巴结转移喉癌中的表达率差异无统计学意义(P>0.05),颈转移淋巴结中NY-ESO-1的表达下调.结论 NY-ESO-1在喉鳞癌患者中特异性高表达,可能以某种方式参与了喉癌的发生、发展,是否能将其作为喉癌免疫治疗靶标有待深入研究.%Objective To detect the expression of Cancer-Testis antigen NY-ESO-1 in human laryngeal squamous carcinoma (LSC) and to explore its significance in immunotherapeutic application. Methods The expressions of NY-ESO-1 protein in the LSC and in the pathologically positive lymph nodes were detected by PV-9000 Immunohistochemistry. Western blotting was also employed to measure the expressions of NY-ESO-1 in the tumor core region(TC), the tissues at the sites of 0.5cm, 1.0cm away from LSC periphery and the distant normal larynx tissues. Results NY-ESO-1 protein expression was positive in 30 out of 69 (43.48 %) cases of LSC. The expression level of NY-ESO-1 protein were found to significantly decrease by tums in TC and corresponding adjacent tissues (P <0.01). None of the nine normal larynx tissues expressed NY-ESO-1 protein.It did not display an obvious correlation between the expression of NY-ESO-1 with T staging, pathological grading and lymph node metastasis (P

  20. NY-ESO-1 Protein Cancer Vaccine With Poly-ICLC and OK-432: Rapid and Strong Induction of NY-ESO-1-specific Immune Responses by Poly-ICLC.

    Science.gov (United States)

    Takeoka, Tomohira; Nagase, Hirotsugu; Kurose, Koji; Ohue, Yoshihiro; Yamasaki, Makoto; Takiguchi, Shuji; Sato, Eiichi; Isobe, Midori; Kanazawa, Takayuki; Matsumoto, Mitsunobu; Iwahori, Kota; Kawashima, Atsunari; Morimoto-Okazawa, Akiko; Nishikawa, Hiroyoshi; Oka, Mikio; Pan, Linda; Venhaus, Ralph; Nakayama, Eiichi; Mori, Masaki; Doki, Yuichiro; Wada, Hisashi

    2017-03-23

    We conducted a clinical trial of a cancer vaccine using NY-ESO-1 protein with polyinosinic-polycytidylic acid-poly-L-lysine carboxymethylcellulose (poly-ICLC) and/or OK-432 against solid tumors. A total of 15 patients were sequentially enrolled in 4 cohorts. Patients in cohort 1 received NY-ESO-1 protein; cohort 2a received NY-ESO-1 protein+OK-432; cohort 2b received NY-ESO-1 protein+poly-ICLC; cohort 3 received NY-ESO-1 protein+OK-432+poly-ICLC with Montanide ISA-51. The endpoints of this trial were safety, NY-ESO-1 immune responses, and clinical response. Vaccine-related adverse events observed were fever and injection-site reaction (grade 1). Two patients showed stable disease after vaccination. NY-ESO-1 antibodies were observed in 4 patients at the baseline (sero-positive) and augmented in all patients after vaccination. Eleven patients showed a conversion of negative antibody responses at baseline to positive after vaccination (seroconversion). The seroconversions were observed in all 11 sero-negative patients by the fourth immunization; in particular, it was observed by the second immunization in patients with poly-ICLC, and these induced antibody responses were stronger than those in patients immunized without poly-ICLC. The number of NY-ESO-1-specific interferon (IFN)γ-producing T cells was increased in patients immunized with poly-ICLC and/or OK-432, and furthermore, the increase of IFNγ-producing CD8 T cells in patients immunized with poly-ICLC was significantly higher than that in patients without poly-ICLC. Nonspecific activations of T-cell or antigen presenting cells were not observed. Our present study showed that poly-ICLC is a promising adjuvant for cancer vaccines.

  1. Molecular cloning, prokaryotic expression and purification of human CT antigen NY-ESO-1%CT抗原NY-ESO-1基因克隆、表达及纯化

    Institute of Scientific and Technical Information of China (English)

    江华; 郑玉玲; 姜永强

    2007-01-01

    目的:利用大肠杆菌表达人CT抗原NY-ESO-1,对表达产物进行Western印迹鉴定和纯化,为今后利用其进行肿瘤疫苗的研究奠定基础.方法:通过全基因拼接获得NY-ESO-1基因,构建重组表达载体NY-ESO-1-pET28a(+),在大肠杆菌BL21(DE3)中利用IPTG诱导获得表达,利用单克隆抗体进行Western印迹鉴定,通过Ni柱亲和纯化获得纯化蛋白.结果与结论:拼接的NY-ESO-1全基因经测序证明与GenBank中人的NY-ESO-l全基因完全相符;构建的原核表达载体NY-ESO-1-pET28a(+)可稳定地可溶性表达NY-ESO-1蛋白;经Ni柱亲和纯化获得较好的纯化结果;利用鼠抗人单克隆抗体对表达的蛋白进行验证,结果显示阳性.本研究成功利用原核表达系统实现了对CT抗原NY-ESO-1的可溶性表达.

  2. Chemical and geochemical composition of spring-summer Arctic aerosol collected at Ny Alesund, Svalbard Islands.

    Science.gov (United States)

    Udisti, Roberto; Becagli, Silvia; Caiazzo, Laura; Cappelletti, David; Giardi, Fabio; Grotti, Marco; Lucarelli, Franco; Moroni, Beatrice; Nava, Silvia; Severi, Mirko; Traversi, Rita

    2017-04-01

    Since March 2010, spring-summer (usually March - September) campaigns were continuously carried out at the Italian Gruvebadet Observatory, Ny Alesund, Svalbard Island. Aerosol was sampled by PM10 (daily) and 4-stage (4-day resolution) collector devices and size distribution was evaluated at 10 min resolution in the range 10 nm - 20 um (106 size classes by a TSI SMPS-APS integrated system). Six-year (2010-2015) PM10 and size-segregated (>10, 10-2.5, 2.5-1, 10.1016/j.atmosenv.2016.04.002. A. Bazzano et al., "Long-range transport of atmospheric lead reaching Ny Alesund: inter-annual and seasonal variations of potential source areas". Atmos. Environ., 2016, 139, 11-19. http://dx.doi.org/10.1016/j.atmosenv.2016.05.026.

  3. Ny krigsførelse i informationsalderen? Om ‘Revolution in Military Affairs’ og Irakkrigen

    Directory of Open Access Journals (Sweden)

    Jens Claus Hansen

    2005-09-01

    Full Text Available Artiklen diskuterer, hvorvidt man efter Golfkrigen kan tale om en ‘Revolu- tion in Military Affairs’ – RMA – som nogle amerikanske krigsteoretikere hævder har fundet sted med anvendelsen af ny teknologi i moderne krigs- førelse. Forfatteren viser, hvordan moderne krigsstrategier har inkorpore- ret ny teknologi og mediebrug som bl.a. informationskrig, ‘Systems of Sys- tems’ og Network Centric Warfare. Forfatteren påpeger, at den strategiske anvendelse af informationsoperationer – herunder ‘embedded’ journalister – ikke har medført en demokratisering af nyhedsdækningen og heller ikke indebærer en øget sandhedsværdi. Afslutningsvis peger forfatteren på behovet for en ‘Revolution in Diplomatic Affairs’.

  4. Characteristics of carbonaceous aerosol in a two-week campaign at Ny-(A)lesund

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The concentrations of organic carbon (OC) and elemental carbon (EC)in total suspended particle (TSP) were investigated at Ny-(A)lesund, Svalbard in a two-week campaign. The levels of OC and EC are 0.86 ±0.27 μgm-3 (mean±standard deviation) and 0. 19±0.10 μgm-3 , respectively. Back trajectory analysis of air masses arriving at Ny-(A)lesund reveals that long-range transport of polluted air play insignificant role in OC and EC levels, to which the potential influence of the local contamination were ascribed. The average OC/EC ratio is 5.41,suggesting the presence of the secondary organic aerosols. The estimated secondary organic carbon (SOC) in TSP is 0.59 μg/m3 , accounting for 64% of the total organic carbon.

  5. CIRCUMBINARY PLANETS ORBITING THE RAPIDLY PULSATING SUBDWARF B-TYPE BINARY NY Vir

    Energy Technology Data Exchange (ETDEWEB)

    Qian, S.-B.; Zhu, L.-Y.; Dai, Z.-B.; He, J.-J. [National Astronomical Observatories/Yunnan Observatory, Chinese Academy of Sciences (CAS), P.O. Box 110, 650011 Kunming (China); Fernandez-Lajus, E. [Facultad de Ciencias Astronomicas y Geofisicas, Universidad Nacional de La Plata, 1900 La Plata, Buenos Aires (Argentina); Xiang, F.-Y., E-mail: qsb@ynao.ac.cn [Physics Department, Xiangtan University, 411105 Xiangtan, Funan Province (China)

    2012-02-15

    We report here the tentative discovery of a Jovian planet in orbit around the rapidly pulsating subdwarf B-type (sdB-type) eclipsing binary NY Vir. By using newly determined eclipse times together with those collected from the literature, we detect that the observed-calculated (O - C) curve of NY Vir shows a small-amplitude cyclic variation with a period of 7.9 yr and a semiamplitude of 6.1 s, while it undergoes a downward parabolic change (revealing a period decrease at a rate of P-dot = -9.2 x 10{sup -12}). The periodic variation was analyzed for the light-travel-time effect via the presence of a third body. The mass of the tertiary companion was determined to be M{sub 3}sin i' = 2.3({+-} 0.3)M{sub Jupiter} when a total mass of 0.60 M{sub Sun} for NY Vir is adopted. This suggests that it is most probably a giant circumbinary planet orbiting NY Vir at a distance of about 3.3 astronomical units (AU). Since the rate of period decrease cannot be explained by true angular momentum loss caused by gravitational radiation or/and magnetic braking, the observed downward parabolic change in the O - C diagram may be only a part of a long-period (longer than 15 years) cyclic variation, which may reveal the presence of another Jovian planet ({approx}2.5 M{sub Jupiter}) in the system.

  6. Immunohistochemical assessment of NY-ESO-1 expression in esophageal adenocarcinoma resection specimens.

    Science.gov (United States)

    Hayes, Stephen J; Hng, Keng Ngee; Clark, Peter; Thistlethwaite, Fiona; Hawkins, Robert E; Ang, Yeng

    2014-04-14

    To assess NY-ESO-1 expression in a cohort of esophageal adenocarcinomas. A retrospective search of our tissue archive for esophageal resection specimens containing esophageal adenocarcinoma was performed, for cases which had previously been reported for diagnostic purposes, using the systematised nomenclature of human and veterinary medicine coding system. Original haematoxylin and eosin stained sections were reviewed, using light microscopy, to confirm classification and tumour differentiation. A total of 27 adenocarcinoma resection specimens were then assessed using immunohistochemistry for NY-ESO-1 expression: 4 well differentiated, 14 moderately differentiated, 4 moderate-poorly differentiated, and 5 poorly differentiated. Four out of a total of 27 cases of esophageal adenocarcinoma examined (15%) displayed diffuse cytoplasmic and nuclear expression for NY-ESO-1. They displayed a heterogeneous and mosaic-type pattern of diffuse staining. Diffuse cytoplasmic staining was not identified in any of these structures: stroma, normal squamous epithelium, normal submucosal gland and duct, Barrett's esophagus (goblet cell), Barrett's esophagus (non-goblet cell) and high grade glandular dysplasia. All adenocarcinomas showed an unexpected dot-type pattern of staining at nuclear, paranuclear and cytoplasmic locations. Similar dot-type staining, with varying frequency and size of dots, was observed on examination of Barrett's metaplasia, esophageal submucosal gland acini and the large bowel negative control, predominantly at the crypt base. Furthermore, a prominent pattern of apical (luminal) cytoplasmic dot-type staining was observed in some cases of Barrett's metaplasia and also adenocarcinoma. A further morphological finding of interest was noted on examination of haematoxylin and eosin stained sections, as aggregates of lymphocytes were consistently noted to surround submucosal glands. We have demonstrated for the first time NY-ESO-1 expression by esophageal

  7. Designprocesser og arbejdsmiljø: Udvikling af ny teknologi til fødevareindustrien

    DEFF Research Database (Denmark)

    Broberg, Ole

    1999-01-01

    Dette papir handler om muligheder og betingelser for at inddrage arbejdsmiljøhensyn i designprocesser. Baggrunden for det er, at jeg har haft mulighed for at følge udviklingen af en ny maskine til fødevareindustrien næsten lige fra de grundlæggende idéer blev udviklet og foreløbig frem til i dag,...

  8. A novel murine T-cell receptor targeting NY-ESO-1.

    Science.gov (United States)

    Rosati, Shannon F; Parkhurst, Maria R; Hong, Young; Zheng, Zhili; Feldman, Steven A; Rao, Mahadev; Abate-Daga, Daniel; Beard, Rachel E; Xu, Hui; Black, Mary A; Robbins, Paul F; Schrump, David A; Rosenberg, Steven A; Morgan, Richard A

    2014-04-01

    Cancer testis antigens, such as NY-ESO-1, are expressed in a variety of prevalent tumors and represent potential targets for T-cell receptor (TCR) gene therapy. DNA encoding a murine anti-NY-ESO-1 TCR gene (mTCR) was isolated from immunized HLA-A*0201 transgenic mice and inserted into a γ-retroviral vector. Two mTCR vectors were produced and used to transduce human PBL. Transduced cells were cocultured with tumor target cell lines and T2 cells pulsed with the NY-ESO-1 peptide, and assayed for cytokine release and cell lysis activity. The most active TCR construct was selected for production of a master cell bank for clinical use. mTCR-transduced PBL maintained TCR expression in short-term and long-term culture, ranging from 50% to 90% efficiency 7-11 days after stimulation and 46%-82% 10-20 days after restimulation. High levels of interferon-γ secretion were observed (1000-12000 pg/mL), in tumor coculture assays and recognition of peptide-pulsed cells was observed at 0.1 ng/mL, suggesting that the new mTCR had high avidity for antigen recognition. mTCR-transduced T cells also specifically lysed human tumor targets. In all assays, the mTCR was equivalent or better than the comparable human TCR. As the functional activity of TCR-transduced cells may be affected by the formation of mixed dimers, mTCRs, which are less likely to form mixed dimers with endogenous hTCRs, may be more effective in vivo. This new mTCR targeted to NY-ESO-1 represents a novel potential therapeutic option for adoptive cell-transfer therapy for a variety of malignancies.

  9. Formulation of Organic Fertilizer Standard in Jiaxing City Based on NY525-2012

    Institute of Scientific and Technical Information of China (English)

    Jinfa; HUANG; Xiongwei; NI; Jianping; XIE; Yanping; SHI

    2013-01-01

    On the basis of research, analysis and argumentation, we conduct analysis and research on the current situation of quality of commercial organic fertilizer in Jiaxing City, and carry out the inspection and testing of organic matter indicators using the laboratory. Based on the standard of NY525-2012 Organic Fertilizer by the Ministry of Agriculture, this paper formulates the quality level indicators of local commercial organic fertilizer and adding specifications of raw materials.

  10. Archive of Datasonics SIS-1000 Boomer Subbottom Data Collected During USGS Cruise DIAN 97011 Long Island, NY Inner Shelf

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY in...

  11. Archive of Datasonics SIS-1000 Boomer Subbottom Data Collected During USGS Cruise DIAN 97011 Long Island, NY Inner Shelf

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY in...

  12. Archive of Datasonics SIS-1000 Boomer Subbottom Data Collected During USGS Cruise DIAN 97011 Long Island, NY Inner Shelf

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS DIAN 97011 cruise. The coverage is the nearshore of Long Island, NY...

  13. Kate Bárány: a life of science, teaching, and service.

    Science.gov (United States)

    Solaro, R John; Rao, Mrinalini C

    2012-12-01

    We celebrate the lives of Michael and Kate Bárány in this issue of the Journal of Muscle Research and Cell Motility. Kate and Michael died within weeks of each other in 2011. Joe Chalovich has written about Michael and we write about Kate. As emphasized by Joe, Kate, and Michael were remarkable individuals who survived the Holocaust, the Hungarian revolution, and emerged from as much adversity as one might imagine to become productive scientists, educators, citizens, and symbols of the durability of the human spirit. They present their own story in an essay (Bárány and Bárány 2000) published in a monograph "Selected Topics in the History of Biochemistry." Rather than repeating much of the list of scientific achievements chronicled in these papers, we focus here on Kate, especially in her role as an individual and partner in science, while at the same time being an accomplished teacher, and a champion of women in science.

  14. Prompt Gamma Activation Analysis of the Nyírlugos obsidian core depot find

    Directory of Open Access Journals (Sweden)

    Zsolt Kasztovszky

    2014-03-01

    Full Text Available The Nyírlugos obsidian core depot find is one of the most important lithic assemblages in the collection of the Hungarian National Museum (HNM. The original set comprised 12 giant obsidian cores, of which 11 are currently on the permanent archaeological exhibition of the HNM. One of the cores is known to be inDebrecen. The first publication attributed the hoard, on the strength of giant (flint blades known from the Early and Middle Copper Age Tiszapolgár and Bodrogkeresztúr cultures, to the Copper Age. In the light of recent finds it is more likely to belong to the Middle Neolithic period. The source area was defined as Tokaj Mts., about100 kmto the NW from Nyírlugos. The size and beauty of the exceptional pieces exclude any invasive analysis. Using Prompt Gamma Activation Analysis (PGAA, we can measure major chemical components and some key trace elements of stone artefacts with adequate accuracy to successfully determine provenance of obsidian. Recent methodological development also facilitated the study of relatively large objects like the Nyírlugos cores. The cores were individually measured by PGAA. The results show that the cores originate from the Carpathian 1 sources, most probably the Viničky variety (C1b. The study of the hoard as a batch is an important contribution to the assessment of prehistoric trade and allows us to reconsider the so-called Carpathian, especially Carpathian 1 (Slovakian sources.

  15. Resiquimod as an Immunologic Adjuvant for NY-ESO-1 Protein Vaccination in Patients with High Risk Melanoma

    Science.gov (United States)

    Gnjatic, Sacha; Cruz, Crystal M.; Vengco, Isabelita; Hasan, Farah; Spadaccia, Meredith; Darvishian, Farbod; Chiriboga, Luis; Holman, Rose Marie; Escalon, Juliet; Muren, Caroline; Escano, Crystal; Yepes, Ethel; Sharpe, Dunbar; Vasilakos, John P.; Rolnitzsky, Linda; Goldberg, Judith; Mandeli, John; Adams, Sylvia; Jungbluth, Achim; Pan, Linda; Venhaus, Ralph; Ott, Patrick A.; Bhardwaj, Nina

    2015-01-01

    The TLR7/8 agonist, Resiquimod has been used as an immune adjuvant in cancer vaccines. We evaluated the safety and immunogenicity of the cancer testis antigen NY-ESO-1 given in combination with Montanide with or without Resiquimod in high-risk melanoma patients. In Part I of the study, patients received 100ug full length NY-ESO-1 protein emulsified in 1.25mL Montanide (day 1) followed by topical application of 1000mg of 0.2% Resiquimod gel on days 1 and 3 (Cohort 1) versus days 1, 3, and 5 (Cohort 2) of a 21 day cycle. In Part II, patients were randomized to receive 100ug NY-ESO-1 protein plus Montanide (day 1) followed by topical application of placebo gel (Arm-A; N=8) or 1000mg of 0.2% Resiquimod gel (Arm-B; N=12) using the dosing regimen established in Part I. The vaccine regimens were generally well-tolerated. NY-ESO-1-specific humoral responses were induced or boosted in all patients, many of whom had high titer antibodies. In Part II, 16 of 20 patients in both arms had NY-ESO-1-specific CD4+ T-cell responses. CD8+ T-cell responses were only seen in 3 of 12 patients in Arm B. Patients with TLR7 SNP rs179008 had a greater likelihood of developing NY-ESO-1-specific CD8+ responses. In conclusion, NY-ESO-1 protein in combination with Montanide with or without topical Resiquimod is safe and induces both antibody and CD4+ T-cell responses in the majority of patients; the small proportion of CD8+ T-cell responses suggests that the addition of topical Resiquimod to Montanide is not sufficient to induce consistent NY-ESO-1-specific CD8+ T-cell responses. PMID:25633712

  16. NY-ESO-1在喉鳞状细胞癌中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    周慧; 凌红阳; 吴文璎; 郑玉双

    2015-01-01

    目的 探讨喉鳞状细胞癌组织中NY-ESO-1蛋白表达及其临床意义.方法 免疫组化方法检测88例喉鳞状细胞癌组织(A组)和10例声带息肉组织(B组)NY-ESO-1蛋白的表达.A组再分为四个亚组,临床分期Ⅰ、Ⅱ期38例(A1组),临床分期Ⅲ、Ⅳ期50例(A2组);病理类型高、中分化60例(A3组),低分化28例(A4组).结果 A组NY-ESO-1蛋白表达水平高于B组(4598±2302 vs.0)(P<0.01).A1组NY-ESO-1表达水平高于A2组(8052±3810 vs.162±107)(P<0.05).A3组NY-ESO-1表达水平高于A4组(5090±1893 vs.211±201) (P<0.05).结论 NY-ESO-1有可能作为喉鳞癌早期诊断的新指标;NY-ESO-1表达阳性的高、中分化喉鳞癌患者可能可以选择NY-ESO-1疫苗作为辅助治疗.

  17. Immune escape from NY-ESO-1-specific T-cell therapy via loss of heterozygosity in the MHC.

    Science.gov (United States)

    Klippel, Z K; Chou, J; Towlerton, A M; Voong, L N; Robbins, P; Bensinger, W I; Warren, E H

    2014-03-01

    Adoptive immunotherapy of tumors with T cells specific for the cancer-testis antigen NY-ESO-1 has shown great promise in preclinical models and in early stage clinical trials. Tumor persistence or recurrence after NY-ESO-1-specific therapy occurs, however, and the mechanisms of recurrence remain poorly defined. In a murine xenograft model of NY-ESO-1(+) multiple myeloma, we observed tumor recurrence after adoptive transfer of CD8(+) T cells genetically redirected to the prototypic NY-ESO-1157-165 peptide presented by HLA-A*02:01. Analysis of the myeloma cells that had escaped from T-cell control revealed intact expression of NY-ESO-1 and B2M, but selective, complete loss of HLA-A*02:01 expression from the cell surface. Loss of heterozygosity (LOH) in the major histocompatibility complex (MHC) involving the HLA-A locus was identified in the tumor cells, and further analysis revealed selective loss of the allele encoding HLA-A*02:01. Although LOH involving the MHC has not been described in myeloma patients with persistent or recurrent disease after immune therapies such as allogeneic hematopoietic cell transplantation (HCT), it has been described in patients with acute myelogenous leukemia who relapsed after allogeneic HCT. These results suggest that MHC loss should be evaluated in patients with myeloma and other cancers who relapse after adoptive NY-ESO-1-specific T-cell therapy.

  18. A cancer vaccine induces expansion of NY-ESO-1-specific regulatory T cells in patients with advanced melanoma.

    Science.gov (United States)

    Ebert, Lisa M; MacRaild, Sarah E; Zanker, Damien; Davis, Ian D; Cebon, Jonathan; Chen, Weisan

    2012-01-01

    Cancer vaccines are designed to expand tumor antigen-specific T cells with effector function. However, they may also inadvertently expand regulatory T cells (Treg), which could seriously hamper clinical efficacy. To address this possibility, we developed a novel assay to detect antigen-specific Treg based on down-regulation of surface CD3 following TCR engagement, and used this approach to screen for Treg specific to the NY-ESO-1 tumor antigen in melanoma patients treated with the NY-ESO-1/ISCOMATRIX™ cancer vaccine. All patients tested had Treg (CD25(bright) FoxP3(+) CD127(neg)) specific for at least one NY-ESO-1 epitope in the blood. Strikingly, comparison with pre-treatment samples revealed that many of these responses were induced or boosted by vaccination. The most frequently detected response was toward the HLA-DP4-restricted NY-ESO-1(157-170) epitope, which is also recognized by effector T cells. Notably, functional Treg specific for an HLA-DR-restricted epitope within the NY-ESO-1(115-132) peptide were also identified at high frequency in tumor tissue, suggesting that NY-ESO-1-specific Treg may suppress local anti-tumor immune responses. Together, our data provide compelling evidence for the ability of a cancer vaccine to expand tumor antigen-specific Treg in the setting of advanced cancer, a finding which should be given serious consideration in the design of future cancer vaccine clinical trials.

  19. Tetramer guided, cell sorter assisted production of clinical grade autologous NY-ESO-1 specific CD8(+) T cells.

    Science.gov (United States)

    Pollack, Seth M; Jones, Robin L; Farrar, Erik A; Lai, Ivy P; Lee, Sylvia M; Cao, Jianhong; Pillarisetty, Venu G; Hoch, Benjamin L; Gullett, Ashley; Bleakley, Marie; Conrad, Ernest U; Eary, Janet F; Shibuya, Kendall C; Warren, Edus H; Carstens, Jason N; Heimfeld, Shelly; Riddell, Stanley R; Yee, Cassian

    2014-01-01

    Adoptive T cell therapy represents an attractive modality for the treatment of patients with cancer. Peripheral blood mononuclear cells have been used as a source of antigen specific T cells but the very low frequency of T cells recognizing commonly expressed antigens such as NY-ESO-1 limit the applicability of this approach to other solid tumors. To overcome this, we tested a strategy combining IL-21 modulation during in vitro stimulation with first-in-class use of tetramer-guided cell sorting to generate NY-ESO-1 specific cytotoxic T lymphocytes (CTL). CTL generation was evaluated in 6 patients with NY-ESO-1 positive sarcomas, under clinical manufacturing conditions and characterized for phenotypic and functional properties. Following in vitro stimulation, T cells stained with NY-ESO-1 tetramer were enriched from frequencies as low as 0.4% to >90% after single pass through a clinical grade sorter. NY-ESO-1 specific T cells were generated from all 6 patients. The final products expanded on average 1200-fold to a total of 36 billion cells, were oligoclonal and contained 67-97% CD8(+), tetramer(+) T cells with a memory phenotype that recognized endogenous NY-ESO-1. This study represents the first series using tetramer-guided cell sorting to generate T cells for adoptive therapy. This approach, when used to target more broadly expressed tumor antigens such as WT-1 and additional Cancer-Testis antigens will enhance the scope and feasibility of adoptive T cell therapy.

  20. Factors Influencing Divergent Patterns of Phosphorus Availability in NY and PA Biogeochemical `Hotspots'

    Science.gov (United States)

    Saia, S. M.; Hofmeister, K.; Regan, J. M.; Buda, A. R.; Carrick, H. J.; Walter, M. T.

    2016-12-01

    Anthropogenic alteration of the soil phosphorus (P) cycle leads to subsequent water quality issues in agricultural dominated watersheds. In the humid Northeastern United States (NE US), variably saturated areas can generate surface runoff that transports P and stimulates biogeochemical processes; these hydrologically dynamic locations are often called biogeochemical `hotspots'. Many studies have evaluated nitrogen and carbon cycling in biogeochemical hot spots but few have focused on P. We hypothesized seasonally wet parts of the landscape (i.e., hotspots) have smaller biologically available P pools because runoff events frequently carry away nutrients like P. To test this hypothesis, we generated soil wetness index (SWI) maps from soil (SURRGO) and elevation (LiDAR rescaled to 3 m) data and used these maps to direct seasonal soil sampling near Klingerstown, Pennsylvania (PA) and Ithaca, New York (NY). We collected 5cm deep soil samples in PA (bimonthly) and NY (monthly) along soil moisture gradients for a range of land cover types (forest, fallow, and cropped) from May through October. We measured soil moisture in the field and percent organic matter (OM), pH, and three increasingly strong soil P extractions (dilute-salt-extractable P, oxalate-extractable P, and total-extractable P) in the laboratory. Our results indicated a negative relationship between dilute-salt-extractable P concentrations and SWI in PA and no relationship between these same variables in NY. We also found positive relationships between each of the three P extractions in PA but only a positive relationship between oxalate-extractable P and total-extractable P in NY. Our findings in PA support our hypothesis; namely, less biologically available P (i.e. dilute-salt-extractable P) is found in wetter areas of the landscape. However, divergent P availability patterns in NY point to further complexities and confounding variables in our understanding in soil P processes. Further studies will look

  1. Efficient in vivo priming by vaccination with recombinant NY-ESO-1 protein and CpG in antigen naive prostate cancer patients.

    Science.gov (United States)

    Karbach, Julia; Neumann, Antje; Atmaca, Akin; Wahle, Claudia; Brand, Kathrin; von Boehmer, Lotta; Knuth, Alexander; Bender, Armin; Ritter, Gerd; Old, Lloyd J; Jäger, Elke

    2011-02-15

    NY-ESO-1, one of the most immunogenic tumor antigens, is expressed in 15% to 25% of metastatic prostate cancers. The immunological and clinical effects of vaccination with recombinant NY-ESO-1 protein combined with CpG as adjuvant were evaluated. In a phase I clinical study, patients with advanced prostate cancer were vaccinated with recombinant NY-ESO-1 protein (100 μg) mixed with CpG 7909 (2.5 mg) every 3 weeks intradermally for 4 doses. Objectives of the study were the safety of the vaccine and changes of specific humoral and cellular immunological responses to NY-ESO-1 in relation to detectable NY-ESO-1 expression in the individual tumor. All 12 baseline sero-negative patients developed high-titer NY-ESO-1 antibody responses. B-cell epitope mapping identified NY-ESO-1 p91-110 to be recognized most frequently by vaccine-induced antibodies. Two patients developed significant antibody titers against the adjuvant CpG. NY-ESO-1-specific CD4+ and/or CD8+ T-cell responses were induced in 9 patients (69%). Five of these 9 patients did not express NY-ESO-1 in the autologous tumor. Postvaccine CD8+ T-cell clones recognized and lyzed HLA-matched tumor cell lines in an antigen-specific manner. Our data provide clear evidence for the capacity of NY-ESO-1 protein/CpG vaccine to induce integrated antigen-specific immune responses in vivo and to efficiently prime CD8+ T-cell responses in NY-ESO-1 antigen-negative patients. Our results may also support further clinical vaccination protocols with NY-ESO-1 protein not only focused on the treatment of existing cancer, but also to prevent further development of NY-ESO-1 positive cancers in vivo. ©2010 AACR.

  2. Dose-dependent effects of NY-ESO-1 protein vaccine complexed with cholesteryl pullulan (CHP-NY-ESO-1) on immune responses and survival benefits of esophageal cancer patients.

    Science.gov (United States)

    Kageyama, Shinichi; Wada, Hisashi; Muro, Kei; Niwa, Yasumasa; Ueda, Shugo; Miyata, Hiroshi; Takiguchi, Shuji; Sugino, Sahoko H; Miyahara, Yoshihiro; Ikeda, Hiroaki; Imai, Naoko; Sato, Eiichi; Yamada, Tomomi; Osako, Masaharu; Ohnishi, Mami; Harada, Naozumi; Hishida, Tadashi; Doki, Yuichiro; Shiku, Hiroshi

    2013-10-05

    Cholesteryl pullulan (CHP) is a novel antigen delivery system for cancer vaccines. This study evaluated the safety, immune responses and clinical outcomes of patients who received the CHP-NY-ESO-1 complex vaccine, Drug code: IMF-001. Patients with advanced/metastatic esophageal cancer were enrolled and subcutaneously vaccinated with either 100 μg or 200 μg of NY-ESO-1 protein complexed with CHP. The primary endpoints were safety and humoral immune responses, and the secondary endpoint was clinical efficacy. A total of 25 patients were enrolled. Thirteen and twelve patients were repeatedly vaccinated with 100 μg or 200 μg of CHP-NY-ESO-1 with a median of 8 or 9.5 doses, respectively. No serious adverse events related to the vaccine were observed. Three out of 13 patients in the 100-μg cohort and 7 out of 12 patients in the 200-μg cohort were positive for anti-NY-ESO-1 antibodies at baseline. In the 100-μg cohort, an antibody response was observed in 5 out of 10 pre-antibody-negatives patients, and the antibody levels were augmented in 2 pre-antibody-positive patients after vaccination. In the 200-μg cohort, all 5 pre-antibody-negative patients became seropositive, and the antibody level was amplified in all 7 pre-antibody-positive patients. No tumor shrinkage was observed. The patients who received 200 μg of CHP-NY-ESO-1 survived longer than patients receiving 100 μg of CHP-NY-ESO-1, even those who exhibited unresponsiveness to previous therapies or had higher tumor burdens. The safety and immunogenicity of CHP-NY-ESO-1 vaccine were confirmed. The 200 μg dose more efficiently induced immune responses and suggested better survival benefits. (Clinical trial registration number NCT01003808).

  3. Induction of CD8 T-cell responses restricted to multiple HLA class I alleles in a cancer patient by immunization with a 20-mer NY-ESO-1f (NY-ESO-1 91-110) peptide.

    Science.gov (United States)

    Eikawa, Shingo; Kakimi, Kazuhiro; Isobe, Midori; Kuzushima, Kiyotaka; Luescher, Immanuel; Ohue, Yoshihiro; Ikeuchi, Kazuhiro; Uenaka, Akiko; Nishikawa, Hiroyoshi; Udono, Heiichiro; Oka, Mikio; Nakayama, Eiichi

    2013-01-15

    Immunogenicity of a long 20-mer NY-ESO-1f peptide vaccine was evaluated in a lung cancer patient TK-f01, immunized with the peptide with Picibanil OK-432 and Montanide ISA-51. We showed that internalization of the peptide was necessary to present CD8 T-cell epitopes on APC, contrasting with the direct presentation of the short epitope. CD8 T-cell responses restricted to all five HLA class I alleles were induced in the patient after the peptide vaccination. Clonal analysis showed that B*35:01 and B*52:01-restricted CD8 T-cell responses were the two dominant responses. The minimal epitopes recognized by A*24:02, B*35:01, B*52:01 and C*12:02-restricted CD8 T-cell clones were defined and peptide/HLA tetramers were produced. NY-ESO-1 91-101 on A*24:02, NY-ESO-1 92-102 on B*35:01, NY-ESO-1 96-104 on B*52:01 and NY-ESO-1 96-104 on C*12:02 were new epitopes first defined in this study. Identification of the A*24:02 epitope is highly relevant for studying the Japanese population because of its high expression frequency (60%). High affinity CD8 T-cells recognizing tumor cells naturally expressing the epitopes and matched HLA were induced at a significant level. The findings suggest the usefulness of a long 20-mer NY-ESO-1f peptide harboring multiple CD8 T-cell epitopes as an NY-ESO-1 vaccine. Characterization of CD8 T-cell responses in immunomonitoring using peptide/HLA tetramers revealed that multiple CD8 T-cell responses comprised the dominant response. Copyright © 2012 UICC.

  4. 靶向克隆法构建NY-ESO-1基因的原核表达载体%Construction of NY-ESO-1 Gene Prokaryotic Expression Vector by LP Recco PCR Cloning

    Institute of Scientific and Technical Information of China (English)

    俞远东; 徐少勇; 王斌; 王家宁; 黄永章

    2009-01-01

    目的:利用PCR产物靶向克隆法构建人癌-睾丸抗原NY-ESO-1的原核表达载体.方法:从人新鲜的食管癌组织中提取总RNA,通过RT-PCR技术扩增NY-ESO-1基因3'端的340 bp片段,采用靶向克隆法将目的基因插入到原核表达载体pET-15b中,得到重组表达质粒pET-15b-NY-ESO-1,经过筛选,挑选出阳性克隆进行Xho Ⅰ和BamHⅠ双酶切图谱分析、PCR检测和扩增产物核苷酸序列分析等,鉴定所构建的原核表达载体.结果:扩增得到NY-ESO-1基因片段,经测序证实与GenBank公布的序列一致,重组的质粒经过PCR,酶切鉴定获得了一个大小为340 bp的特征性片段,证明重组质粒中已成功的插入了目的基因NY-ESO-1.结论:靶向克隆法可快速、高效地构建人NY-ESO-1基因的原核表达载体,为制备pET-15b-NY-ESO-1融合蛋白奠定了基础.

  5. Combination of autoantibodies against NY-ESO-1 and viral capsid antigen immunoglobulin A for improved detection of nasopharyngeal carcinoma.

    Science.gov (United States)

    Peng, Yu-Hui; Xu, Yi-Wei; Qiu, Si-Qi; Hong, Chao-Qun; Zhai, Tian-Tian; Li, En-Min; Xu, Li-Yan

    2014-09-01

    Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in Southern China and Southeast Asia, and early detection remains a challenge. Autoantibodies have been found to precede the manifestations of symptomatic cancer by several months to years, making their identification of particular relevance for early detection. In the present study, the diagnostic value of serum autoantibodies against NY-ESO-1 in NPC patients was evaluated. The study included 112 patients with NPC and 138 normal controls. Serum levels of autoantibodies against NY-ESO-1 and classical Epstein-Barr virus marker, viral capsid antigen immunoglobulin A (VCA-IgA), were measured by enzyme-linked immunosorbent assay. Measurement of autoantibodies against NY-ESO-1 and VCA-IgA demonstrated a sensitivity/specificity of 42.9/94.9% [95% confidence interval (CI), 33.7-52.6/89.4-97.8%] and 55.4/95.7% (95% CI, 45.7-64.7/90.4-98.2%), respectively. The area under receiver operating characteristic curve for autoantibodies against NY-ESO-1 (0.821; 95% CI, 0.771-0.871) was marginally lower than that for VCA-IgA (0.860; 95% CI, 0.810-0.910) in NPC. The combination of autoantibodies against NY-ESO-1 and VCA-IgA yielded an enhanced sensitivity of 80.4% (95% CI, 71.6-87.0%) and a specificity of 90.6% (95% CI, 84.1-94.7%). Moreover, detection of autoantibodies against NY-ESO-1 could differentiate early-stage NPC patients from normal controls. Our results suggest that autoantibodies against NY-ESO-1 may serve as a potential biomarker, as a supplement to VCA-IgA, for the screening and diagnosis of NPC.

  6. Cancer-testis antigen expression in synovial sarcoma: NY-ESO-1, PRAME, MAGEA4, and MAGEA1.

    Science.gov (United States)

    Iura, Kunio; Maekawa, Akira; Kohashi, Kenichi; Ishii, Takeaki; Bekki, Hirofumi; Otsuka, Hiroshi; Yamada, Yuichi; Yamamoto, Hidetaka; Harimaya, Katsumi; Iwamoto, Yukihide; Oda, Yoshinao

    2017-03-01

    Synovial sarcoma (SS) is regarded as a relatively chemosensitive sarcoma, but the prognosis of advanced SSs remains poor. Here we identified highly expressed cancer-testis antigens that could be promising immunotherapy targets for SS, using a previously conducted cDNA microarray, and we assessed the clinicopathological or prognostic relationships of these antigens in SS. We compared the gene expression profiles of 11 SSs with those of 3 normal adipose tissues. Among the up-regulated cancer-testis antigens, we analyzed PRAME, MAGEA1, and MAGEA4 and another cancer-testis antigen (NY-ESO-1) together, by immunohistochemistry and real-time polymerase chain reaction in 108 SSs. Immunohistochemically, NY-ESO-1, PRAME, MAGEA4, and MAGEA1 were positive in 66 (61%), 93 (86%), 89 (82%), and 16 (15%) of 108 SSs, respectively, and 104 (96%) of 108 SSs showed the immunohistochemical expression of at least 1 of NY-ESO-1, PRAME, and MAGEA4. Moreover, the high expression of at least 1 of these 3 antigens was observed in 83% of the SSs. High expression of NY-ESO-1 and MAGEA4 was significantly correlated with the presence of necrosis and advanced clinical stage. The immunohistochemical expression of these cancer-testis antigens was not correlated with prognosis, but the coexpression of NY-ESO-1, PRAME, and MAGEA4 was significantly associated with adverse prognosis. The real-time polymerase chain reaction results were closely related to the immunohistochemical results: NY-ESO-1 (P = .0019), PRAME (P = .039), MAGEA4 (P = .0149), and MAGEA1 (P = .0766). These data support the potential utility of NY-ESO-1, PRAME, and MAGEA4 as immunotherapy targets and ancillary prognostic parameters, suggesting the possible benefit of the combined use of these cancer-testis antigens as an SS immunotherapy target. Copyright © 2016 Elsevier Inc. All rights reserved.

  7. Functional autoantibodies against SSX-2 and NY-ESO-1 in multiple myeloma patients after allogeneic stem cell transplantation.

    Science.gov (United States)

    Luetkens, Tim; Kobold, Sebastian; Cao, Yanran; Ristic, Marina; Schilling, Georgia; Tams, Sinje; Bartels, Britta Marlen; Templin, Julia; Bartels, Katrin; Hildebrandt, York; Yousef, Sara; Marx, Andreas; Haag, Friedrich; Bokemeyer, Carsten; Kröger, Nicolaus; Atanackovic, Djordje

    2014-11-01

    Multiple myeloma (MM) is the malignancy with the most frequent expression of the highly immunogenic cancer-testis antigens (CTA), and we have performed the first analysis of longitudinal expression, immunological properties, and fine specificity of CTA-specific antibody responses in MM. Frequency and characteristics of antibody responses against cancer-testis antigens MAGE-A3, NY-ESO-1, PRAME, and SSX-2 were analyzed using peripheral blood (N = 1094) and bone marrow (N = 200) plasma samples from 194 MM patients. We found that antibody responses against CTA were surprisingly rare, only 2.6 and 3.1 % of patients evidenced NY-ESO-1- and SSX-2-specific antibodies, respectively. NY-ESO-1-specific responses were observed during disease progression, while anti-SSX-2 antibodies appeared after allogeneic stem cell transplantation and persisted during clinical remission. We found that NY-ESO-1- and SSX-2-specific antibodies were both capable of activating complement and increasing CTA uptake by antigen-presenting cells. SSX-2-specific antibodies were restricted to IgG3, NY-ESO-1 responses to IgG1 and IgG3. Remarkably, NY-ESO-1-positive sera recognized various non-contiguous regions, while SSX-2-specific responses were directed against a single 6mer epitope, SSX-2(85-90). We conclude that primary autoantibodies against intracellular MM-specific tumor antigens SSX-2 and NY-ESO-1 are rare but functional. While their contribution to disease control still remains unclear, our data demonstrate their theoretic ability to affect cellular anti-tumor immunity by formation and uptake of mono- and polyvalent immune complexes.

  8. NY-ESO-1 Vaccination in Combination with Decitabine Induces Antigen-Specific T-Lymphocyte Responses in Patients with Myelodysplastic Syndrome.

    Science.gov (United States)

    Griffiths, Elizabeth A; Srivastava, Pragya; Matsuzaki, Junko; Brumberger, Zachary; Wang, Eunice S; Kocent, Justin; Miller, Austin; Roloff, Gregory W; Wong, Hong Yuen; Paluch, Benjamin E; Lutgen-Dunckley, Linda G; Martens, Brandon L; Odunsi, Kunle; Karpf, Adam R; Hourigan, Christopher S; Nemeth, Michael J

    2017-09-25

    Treatment options are limited for patients with high-risk myelodysplastic syndrome (MDS). The azanucleosides, azacitidine and decitabine, are front-line therapy for MDS that induce promoter demethylation and gene expression of the highly immunogenic tumor antigen NY-ESO-1. We demonstrated that AML patients receiving decitabine exhibit induction of NY-ESO-1 expression in circulating blasts. We hypothesized that vaccinating against NY-ESO-1 in MDS patients receiving decitabine would capitalize upon induced NY-ESO-1 expression in malignant myeloid cells to provoke an NY-ESO-1-specific-MDS directed cytotoxic T-cell immune response. In a phase I study, 9 MDS patients received an HLA unrestricted NY-ESO-1 vaccine (CDX-1401 + poly-ICLC) in a non-overlapping schedule every four weeks with standard dose decitabine. Analysis of samples serially obtained from the 7 patients who reached the end-of-study demonstrated induction of NY-ESO-1 expression in 7/7 patients and NY-ESO-1 specific CD4+ and CD8+ T-lymphocyte responses in 6/7 and 4/7 of the vaccinated patients respectively. Myeloid cells expressing NY-ESO-1, isolated from a patient at different time-points during decitabine therapy, were capable of activating a cytotoxic response from autologous NY-ESO-1-specific T-lymphocytes. Vaccine responses were associated with a detectable population of CD141Hi conventional dendritic cells, which are critical for the uptake of NY-ESO-1 vaccine and have a recognized role in anti-tumor immune responses. These data indicate that vaccination against induced NY-ESO-1 expression can produce an antigen-specific immune response in a relatively non-immunogenic myeloid cancer and highlight the potential for induced-antigen directed immunotherapy in a group of patients with limited options. Copyright ©2017, American Association for Cancer Research.

  9. Håndværk og design; nyt fag, ny didaktik – et aktionsforskningsprojekt

    Directory of Open Access Journals (Sweden)

    Stig Pedersen

    2011-11-01

    Full Text Available Projektet tager afsæt i regeringens forslag om at sammenlægge fagene sløjd og håndarbejde til et nyt fag ”håndværk og design”. Det er projektets ambition at være dagsordensættende i forhold til udvikling af dette nye skolefag. Vi vil arbejde ud fra en aktionsforskningsmæssig tilgang. Det betyder, at vi ikke alene har til hensigt at etablere et ”objektivt” billede af, hvordan udvalgte skoler arbejder med at udvikle det ny fag ”håndværk og design”, men ønsker at påvirke udviklingen og dermed i fællesskab konstruere ny didaktisk teori. Som støtte for projektgruppens arbejde nedsættes en metodisk, didaktisk studiekreds, hvor projektgruppen modtager sparring fra erfarne forskere. Det er projektets sigte at undersøge og udvikle det ny fags didaktik med henblik på at generere visioner for bl.a. indholdsudvælgelse og læreformer i et fremtidsperspektiv for faget ”håndværk og design” i samspil mellem de ideer, viden og erfaringer forskerne har, den viden og erfaring de udvalgte forskende lærere har, samt de erfaringer og ideer, der opbygges i projektets praksisdele. Der arbejdes med eksemplariske forløb, som planlægges og efterbehandles i samarbejde mellem lærere og forskere. Dette samarbejde dokumenteres af lærerne og projektgruppens medlemmer i fællesskab. Her indgår fx undervisningsoplæg, materialer og genstande, evalueringsformer – og resultater samt fokusgruppe interview.Søkord: metod, sløjd, håndværk og design, aktionsforskning, folkeskolenURN:NBN:no-29962

  10. Framtagning av ny lyftytrystning för gasturbin SGT-600/700

    OpenAIRE

    Sjöberg, Emma

    2008-01-01

    I detta Examensarbete har en ny lyftutrustning till gasturbin SGT-600/700 olika moduler tagits fram. Examensarbetet har utförts på Siemens Industrial Turbomachinery AB i Finspång på avdelningen GPTP (leverans gasturbin teknik package installation). Målet med arbetet har varit att skapa en lyftutrustning som är mer flexibel än den befintliga lyftutrustningen och klarar samlyft (lyft med hjälp av två kranar). Arbetet har utförts med hjälp av olika metoder som QFD, funktionsmedel-träd och morpho...

  11. Primes of the form x2+ny2 Fermat, class field theory, and complex multiplication

    CERN Document Server

    Cox, David A

    2014-01-01

    An exciting approach to the history and mathematics of number theory ". . . the author's style is totally lucid and very easy to read . . .the result is indeed a wonderful story." -Mathematical ReviewsWritten in a unique and accessible style for readers of varied mathematical backgrounds, the Second Edition of Primes of the Form p = x2+ ny2 details the history behind how Pierre de Fermat's work ultimately gave birth to quadratic reciprocity and the genus theory of quadratic forms. The book also illustrates how results of Euler and Gauss can be fully understood only in the context of class fi

  12. Boktrailern – : En kartläggning av en ny medieprodukt

    OpenAIRE

    Rybin, Claudia

    2015-01-01

    I detta examensarbete kartlägger jag en ny medieprodukt, boktrailern. En boktrailer är en audiovisuell reklam för en bok och härstammar från samt följer dels samma konventioner som den etablerade filmtrailertraditionen. I vissa fall kan boktrailern även ses som en självständig kortfilm. Boktrailern har uppkommit vid sekelskiftet och utvecklats som en följd av tre faktorer kopplade till tjugohundratalets teknologiska framsteg: 1. Verbal text är allt mer tvungen att förlita sig på ...

  13. Decitabine immunosensitizes human gliomas to NY-ESO-1 specific T lymphocyte targeting through the Fas/Fas Ligand pathway

    Science.gov (United States)

    2011-01-01

    Background The lack of effective treatments for gliomas makes them a significant health problem and highlights the need for the development of novel and innovative treatment approaches. Immunotherapy is an appealing strategy because of the potential ability for immune cells to traffic to and destroy infiltrating tumor cells. However, the absence of well-characterized, highly immunogenic tumor-rejection antigens (TRA) in gliomas has limited the implementation of targeted immune-based therapies. Methods We hypothesized that treatment with the demethylating agent, decitabine, would upregulate the expression of TRA on tumor cells, thereby facilitating enhanced surveillance by TRA-specific T cells. Results and Discussion Treatment of human glioma cells with decitabine increased the expression of NY-ESO-1 and other well characterized cancer testes antigens. The upregulation of NY-ESO-1 made these tumors susceptible to NY-ESO-1-specific T-cell recognition and lysis. Interestingly, decitabine treatment of T98 glioma cells also sensitized them to Fas-dependent apoptosis with an agonistic antibody, while a Fas blocking antibody could largely prevent the enhanced functional recognition by NY-ESO-1 specific T cells. Thus, decitabine treatment transformed a non-immunogenic glioma cell into an immunogenic target that was efficiently recognized by NY-ESO-1--specific T cells. Conclusions Such data supports the hypothesis that agents which alter epigenetic cellular processes may "immunosensitize" tumor cells to tumor-specific T cell-mediated lysis. PMID:22060015

  14. Isolation of a Halophilic Bacterium, Bacillus sp. Strain NY-6 for Organic Contaminants Removal in Saline Wastewater on Ship

    Institute of Scientific and Technical Information of China (English)

    Jie Gao; Zhenjiang Yu; Xiaohui Zhang; Dan Zhao; Fangbo Zhao

    2013-01-01

    The objective of this research was to examine if certain strains of Bacillus bacteria,could survive in dry powder products and if so,could the bacteria degrade organic contaminants in saline wastewater on a ship.As part of the study,we isolated 7 domesticated strains named NY1,NY2,…,and NY7,the strain NY6 showed to have the best performance for organic matter degradation and could survive in dry powder more than 3 months.NY6 was identified as Bacillus aerius,based on the morphological and physic-chemical properties.Its optimal growth conditions were as follows:salinity was 2%; temperature was 37℃; pH was in 6.5-7.0; best ratio of C∶ N∶ P was 100∶5∶1.The capability of its dry powder for Chemical Oxygen Demand (COD) removal was 800mg COD/g in synthesized marine wastewater with 2% salinity.The spores in the dry powder were 1.972× 108 g1.

  15. Decitabine immunosensitizes human gliomas to NY-ESO-1 specific T lymphocyte targeting through the Fas/Fas Ligand pathway

    Directory of Open Access Journals (Sweden)

    Konkankit Veerauo V

    2011-11-01

    Full Text Available Abstract Background The lack of effective treatments for gliomas makes them a significant health problem and highlights the need for the development of novel and innovative treatment approaches. Immunotherapy is an appealing strategy because of the potential ability for immune cells to traffic to and destroy infiltrating tumor cells. However, the absence of well-characterized, highly immunogenic tumor-rejection antigens (TRA in gliomas has limited the implementation of targeted immune-based therapies. Methods We hypothesized that treatment with the demethylating agent, decitabine, would upregulate the expression of TRA on tumor cells, thereby facilitating enhanced surveillance by TRA-specific T cells. Results and Discussion Treatment of human glioma cells with decitabine increased the expression of NY-ESO-1 and other well characterized cancer testes antigens. The upregulation of NY-ESO-1 made these tumors susceptible to NY-ESO-1-specific T-cell recognition and lysis. Interestingly, decitabine treatment of T98 glioma cells also sensitized them to Fas-dependent apoptosis with an agonistic antibody, while a Fas blocking antibody could largely prevent the enhanced functional recognition by NY-ESO-1 specific T cells. Thus, decitabine treatment transformed a non-immunogenic glioma cell into an immunogenic target that was efficiently recognized by NY-ESO-1--specific T cells. Conclusions Such data supports the hypothesis that agents which alter epigenetic cellular processes may "immunosensitize" tumor cells to tumor-specific T cell-mediated lysis.

  16. Recombinant Lactobacillus plantarum induces immune responses to cancer testis antigen NY-ESO-1 and maturation of dendritic cells.

    Science.gov (United States)

    Mobergslien, Anne; Vasovic, Vlada; Mathiesen, Geir; Fredriksen, Lasse; Westby, Phuong; Eijsink, Vincent G H; Peng, Qian; Sioud, Mouldy

    2015-01-01

    Given their safe use in humans and inherent adjuvanticity, Lactic Acid Bacteria may offer several advantages over other mucosal delivery strategies for cancer vaccines. The objective of this study is to evaluate the immune responses in mice after oral immunization with Lactobacillus (L) plantarum WCFS1 expressing a cell-wall anchored tumor antigen NY-ESO-1. And to investigate the immunostimulatory potency of this new candidate vaccine on human dendritic cells (DCs). L. plantarum displaying NY-ESO-1 induced NY-ESO-1 specific antibodies and T-cell responses in mice. By contrast, L. plantarum displaying conserved proteins such as heat shock protein-27 and galectin-1, did not induce immunity, suggesting that immune tolerance to self-proteins cannot be broken by oral administration of L. plantarum. With respect to immunomodulation, immature DCs incubated with wild type or L. plantarum-NY-ESO-1 upregulated the expression of co-stimulatory molecules and secreted a large amount of interleukin (IL)-12, TNF-α, but not IL-4. Moreover, they upregulated the expression of immunosuppressive factors such as IL-10 and indoleamine 2,3-dioxygenase. Although L. plantarum-matured DCs expressed inhibitory molecules, they stimulated allogeneic T cells in-vitro. Collectively, the data indicate that L. plantarum-NY-ESO-1 can evoke antigen-specific immunity upon oral administration and induce DC maturation, raising the potential of its use in cancer immunotherapies.

  17. Progress of NY ESO-1 in immunotherapy of hepatocellular carcinoma%NY-ESO-1在原发性肝癌免疫治疗中的研究进展

    Institute of Scientific and Technical Information of China (English)

    夏士珊; 向晓星

    2010-01-01

    NY-ESO-1抗原是CT抗原家族中的重要成员,在原发性肝癌组织中广泛表达,是迄今发现的最具免疫原性的肿瘤抗原,能在NY-ESO-1表达阳性的肝癌患者体内诱导自发性体液免疫反应和特异性T细胞免疫反应.因此,NY-ESO-1被认为是较好的肿瘤候选疫苗,在原发性肝癌免疫治疗中较具发展潜力.

  18. Surveys of mental health status in middle-aged patients from medical dept. of general hospital%综合医院内科门诊中年患者人群心理健康状况调查

    Institute of Scientific and Technical Information of China (English)

    龙本栋; 区丽梅; 冯小佩; 温添娣; 钟玉莲; 钟凤英

    2009-01-01

    Objective To understand the mental health status in middle-aged patients from medical dept. of general hospital. Methods 240 middle-aged patients selected from medical dept. of our hospital were assigned to observation group and 80 middle-aged healthy examinees to control group. Questionnaire investigations were conducted with the Hospital Anxiety and Depression Scale(HADS), Hamilton Anxiety Scale(HAMA) and Hamilton Depression Scale(HAMD),and the mental health status of the 2 groups compared. Results Prevances of psychological disorders were respectively 40.0% in the observation and 10.0% in the control group,which showed a significant difference(P<0.01); those of anxiety and depression were respectively 24.2% and 20.8%. Conclusion The mental health status of middle-aged patients in medical clinic is worse and needs social recognition.%目的 了解综合医院内科门诊中年患者的心理健康状况. 方法 选取我院内科门诊中年患者240例为观察组及中年健康体检者80例为对照组.采用综合性医院焦虑抑郁量表、汉密顿焦虑量表、汉密顿抑郁量表进行问卷调查,并比较两组的心理健康状况. 结果 内科门诊中年患者心理障碍患病率为40.0%,对照组心理障碍患病率为10.0%,两组比较差异有极显著性(P<0.01);其中焦虑、抑郁患病率分别为24.2%、20.8%. 结论 综合医院内科门诊中年患者心理健康状况较差,需引起社会的重视.

  19. PEROMBAKAN SENYAWA HIDROKARBON AROMATIS POLISIKLIK (NAFTALEN PADA KADAR TINGGI OLEH Pseudomonas NY-I (Biodegradation of Polycyclic Aromatic Hydrocarbon (Naphthalene at High Concentration by Pseudomonas NY-1

    Directory of Open Access Journals (Sweden)

    Yanisworo Wijayaratih

    2001-12-01

    Full Text Available ABSTRAK Naftalen merupakan salah satu senyawa hidrokarbon aromatis polisiklik (HAP yang banyak dijumpai dalam minyak bumi, batu bara dan hasil alam lainnya. Meskipun bukan senyawa xenobiotik, naftalen dapat menjadi persoalan yang serius karena penggunaannya yang luas dan penanganan yang tidak hati-hati. Naftalen diketahui bersifat mutagenik. Penelitian ini dilakukan untuk mendapatkan isolat bakteri yang dapat merombak naftalen dan mempelajari kemampuannya merombak naftalen kadar tinggi dalam medium mineral (MM cair. Tanah yang tercemari minyak bumi dan sumber isolat diperoleh dari unit pengolah minyak Pertamina, Cilacap. Isolat dipreroleh melalui kultur diperkaya menggunakan naftalen. Jumlah naftalen yang ditambahkan ke dalam MM cair sebesar 907, 1362 dan 1813 ppm. Inkubasi dilakukan selama 28 hari dalam keadaan gelap. Parameter yang diamati meliputi: jumlah sel hidup dengan metode drop plate dan kadar naftalen sisa dengan menggunakan GC. Hasil penelitian menunjukkan bahwa isolat bakteri yang dipilih, teridentifikasi sebagai Pseudomonas NY-l. Dalam MM cair, naftalen pada semua konsentrasi terombak pada kecepatan yang mirip. Jumlah naftalen yang terombak adalah 777,3 ppm, 728,6 ppm dan 837,2 ppm dari konsentrasi awal berturut-turut sebesar 907, 1362, dan 1813 ppm.   ABSTRACT Naphthalene is one of the Polycyclic Aromatic Hydrocarbons (PAHs, found in petroleum, coal and other natural products. although, it is nonxenobiotic, it could cause a serious problem when improperly used and handled. It is considered as a mutagenic compound. This study is primarily concerned with the isolation of bacteria that could utilize naphthalene and the investigation of its biodegradation ability of naphthalene in high concentration in liquid mineral media (MM. The contaminated soil and isolates were obtained from oil treatment unit, Pertamina, Cilacap. Bacterial isolation was conducted through enriched culture. Naphthalene was added to the liquid MM at the

  20. On the Diophantine equation (12n)x+(35n)y=(37n)z%关于丢番图方程(12n)x(35n)y=(37n)z

    Institute of Scientific and Technical Information of China (English)

    杨志娟; 翁建欣

    2012-01-01

      Using the properties of congruences and the order of elements, we show that, for any positive integer n, the Diophantine equation (12n)x+(35n)y =(37n)z has no solution other than (x, y, z)=(2, 2, 2) in positive integers.%  运用同余及元素阶的性质,证明对任意正整数n,丢番图方程(12n)x+(35n)y=(37n)z仅有正整数解(x, y, z)=(2,2,2)。

  1. NY-ESO-1基因的原核表达、纯化和初步应用%The Prokaryotic Expression, Purification and Preliminary Application of Human NY-ESO-1 Gene

    Institute of Scientific and Technical Information of China (English)

    张文敏; 肖刚; 张萌; 谢丹; 郭爱林; 文剑明

    2005-01-01

    目的:克隆人NY-ESO-1基因,进行原核表达和分离纯化,并初步应用于肝癌患者血清NY-ESO-1抗体的检测.方法:通过RT-PCR技术从人肝癌组织中扩增NY-ESO-1基因片段,插入原核表达载体pGEX4T-1(+)中,构建重组表达质粒pGEX/ESO1,导入大肠杆菌BL21(DE3),诱导目的蛋白表达,经包涵体透析复性和GSTrap亲和层析纯化目的蛋白,Western blot鉴定.应用间接ELISA方法初步检测50例肝癌患者血清和20例正常人血清NY-ESO-1抗体的表达.结果:扩增得到NY-ESO-1基因3'端276bp片段,与GeneBank公布序列一致,构建的pGEX/ESO1原核表达质粒经IPTG诱导,在大肠杆菌中表达分子量约36kD的GST-ESO1融合蛋白,纯化后蛋白纯度为90%.50例肝细胞癌患者血清中4例检测到NY-ESO-1抗体(8%),正常人血清均为阴性.结论:成功构建pGEX/ESO1重组表达质粒,得到有活性的NY-ESO-1融合蛋白,对肝癌患者血清NY-ESO-1抗体的检测有一定的应用价值.

  2. Expression of NY-ESO-1 Gene in Human Bladder Cancer%NY-ESO-1基因在膀胱癌中的表达及临床意义的研究

    Institute of Scientific and Technical Information of China (English)

    王慕文; 毕东滨; 金讯波; 马庆铮

    2010-01-01

    目的 探讨NY-ESO-1的表达与膀胱癌分期分级的关系以及临床意义.方法 回顾性研究2001~2002年膀胱癌组织32例,其中男性24例,女性8例.正常膀胱粘膜对照取自耻骨上经膀胱前列腺切除术的良性前列腺增生的患者.阳性对照为正常睾丸组织.常规行RT-PCR方法检测膀胱癌组织及对照组等NY-ESO-1基因的表达,分析其表达与膀胱癌分期分级的关系以及临床意义.结果 NY-ESO-1 mRNA在32例膀胱癌组织中12例表达(37.5%),在正常膀胱粘膜上没有表达.肿瘤组织中PCR反应的产物与正常睾丸的表达产物一致.NY-ESO-1 mRNA的表达和膀胱癌的病理分级相关,有统计学差异(P<0.05).NY-ESO-1 mRNA的表达率随着肿瘤病理分级的增高而升高:G1期是0/3(0%);G2期是4/16(25%);G3期是8/13(61.54%).浸润性膀胱癌中NY-ESO-1 mRNA的表达与表浅性肿瘤的表达无显著性差异.结论 NY-ESO-1 mRNA在正常膀胱组织上没有表达,仅在正常睾丸组织和病理膀胱癌组织上有表达.NY-ESO-1 mRNA的表达率随着膀胱癌病理分级的增高而升高.

  3. Thermal infrared observations of near-Earth asteroid 2002 NY40

    CERN Document Server

    Müller, T G; Schuetz, O; Pravec, P; Siebenmorgen, R

    2004-01-01

    We obtained N-band observations of the Apollo asteroid 2002 NY40 during its close Earth fly-by in August 2002 with TIMMI2 at the ESO 3.6 m telescope. The photometric measurement allowed us to derive a radiometric diameter of 0.28+/-0.03 km and an albedo of 0.34+/-0.06 through the near-Earth asteroid thermal model (NEATM) and a thermophysical model (TPM). The values are in agreement with results from radar data, visual and near-IR observations. In this first comparison between these two model approaches we found that the empirical NEATM beaming parameter $\\eta$=1.0 corresponds to a thermal inertia values of about 100 $\\mathrm{J m^{-2} s^{-0.5} K^{-1}}$ for a typical range of surface roughness, assuming an equator-on viewing angle. Our TPM analysis indicated that the surface of 2002 NY40 consists of rocky material with a thin or no dust regolith. The asteroid very likely has a prograde sense of rotation with a cold terminator at the time of our observations. Although both model approaches can fit the thermal sp...

  4. Evaluation of LAGE-1 and NY-ESO-1 expression in multiple myeloma patients to explore possible benefits of their homology for immunotherapy.

    Science.gov (United States)

    de Carvalho, Fabricio; Vettore, Andre L; Inaoka, Riguel J; Karia, Bruno; Andrade, Valéria C C; Gnjatic, Sacha; Jungbluth, Achim A; Colleoni, Gisele W B

    2011-01-20

    Due to the high homology between the LAGE-1 and NY-ESO-1 proteins, we hypothesized that an anti-NY-ESO-1 vaccine might elicit LAGE-1 immunity and hence may be effective in multiple myeloma (MM) patients with LAGE-1-positive/NY-ESO-1-negative tumors. Therefore, we set out to evaluate LAGE-1 and NY-ESO-1 mRNA and protein expression in MM patients in a bid to evaluate possible benefits of their homology for immunotherapy. LAGE-1 (a and b isoforms) and NY-ESO-1 mRNA expression was studied in 18 normal tissues and 50 bone marrow MM samples by RT-PCR. LAGE-1 and NY-ESO-1 protein expression was analyzed by immunohistochemistry (IHC) in 27 MM specimens using mAbs 219-510-23 and E978. Spontaneous serological immune response against both antigens was analyzed by ELISA in sera from 33 MM patients. LAGE-1 (a and b isoforms) was positive in 42% and NY-ESO-1 in 26% of the MM samples analyzed by RT-PCR. Both genes were found to be expressed in 18% of the cases, while at least one of the genes was found to be expressed in 50% of the cases. In LAGE-1 positive samples, 81% were positive for LAGE-1a and 19% were positive for both LAGE-1a and -1b. LAGE-1 and NY-ESO-1 protein expression could only be detected in two cases by IHC and there was a clear strong spontaneous antibody response to LAGE-1 and NY-ESO-1 in only one MM patient. In conclusion, LAGE-1a and NY-ESO-1 homology cannot be easily exploited in an anti-NY-ESO-1 vaccine given the low frequency of protein expression detected by IHC or serum analysis.

  5. Measurement of serum antibodies against NY-ESO-1 by ELISA: A guide for the treatment of specific immunotherapy for patients with advanced colorectal cancer.

    Science.gov (United States)

    Long, Yan-Yan; Wang, Yu; Huang, Qian-Rong; Zheng, Guang-Shun; Jiao, Shun-Chang

    2014-10-01

    NY-ESO-1 has been identified as one of the most immunogenic antigens; thus, is a highly attractive target for cancer immunotherapy. The present study analyzed the expression of serum antibodies (Abs) against NY-ESO-1 in patients with advanced colorectal cancer (CRC), with the aim of guiding the treatment of NY-ESO-1-based specific-immunotherapy for these patients. Furthermore, the present study was the first to evaluate the kinetic expression of anti-NY-ESO-1 Abs and investigate the possible influencing factors. A total of 239 serum samples from 155 pathologically confirmed patients with advanced CRC (stages III and IV) were collected. The presence of spontaneous Abs against NY-ESO-1 was analyzed using an enzyme-linked immunosorbent assay (ELISA). The results demonstrated that 24.5% (38/155) of the investigated patients were positive for NY-ESO-1-specific Abs. No statistically significant correlations were identified between the expression of anti-NY-ESO-1 Abs and clinicopathological parameters, including age and gender, location, grading, local infiltration, lymph node status, metastatic status and K-ras mutation status (P>0.05). In 59 patients, the kinetic expression of anti-NY-ESO-1 Abs was analyzed, of which 14 patients were initially positive and 45 patients were initially negative. Notably, 16/59 (27.1%) patients changed their expression status during the study period, and the initially positive patients were more likely to change compared with the initially negative patients (85.7 vs. 8.8%; PESO-1 by ELISA is an easy and feasible method. The high expression rate of NY-ESO-1-specific Abs in CRC patients indicates that measuring the levels of serum Abs against NY-ESO-1 may guide the treatment of NY-ESO-1-based specific immunotherapy for patients with advanced CRC.

  6. Integrated NY-ESO-1 antibody and CD8+ T-cell responses correlate with clinical benefit in advanced melanoma patients treated with ipilimumab.

    Science.gov (United States)

    Yuan, Jianda; Adamow, Matthew; Ginsberg, Brian A; Rasalan, Teresa S; Ritter, Erika; Gallardo, Humilidad F; Xu, Yinyan; Pogoriler, Evelina; Terzulli, Stephanie L; Kuk, Deborah; Panageas, Katherine S; Ritter, Gerd; Sznol, Mario; Halaban, Ruth; Jungbluth, Achim A; Allison, James P; Old, Lloyd J; Wolchok, Jedd D; Gnjatic, Sacha

    2011-10-04

    Ipilimumab, a monoclonal antibody against cytotoxic T lymphocyte antigen 4 (CTLA-4), has been shown to improve survival in patients with advanced metastatic melanoma. It also enhances immunity to NY-ESO-1, a cancer/testis antigen expressed in a subset of patients with melanoma. To characterize the association between immune response and clinical outcome, we first analyzed NY-ESO-1 serum antibody by ELISA in 144 ipilimumab-treated patients with melanoma and found 22 of 140 (16%) seropositive at baseline and 31 of 144 (22%) seropositive following treatment. These NY-ESO-1-seropositive patients had a greater likelihood of experiencing clinical benefit 24 wk after ipilimumab treatment than NY-ESO-1-seronegative patients (P = 0.02, relative risk = 1.8, two-tailed Fisher test). To understand why some patients with NY-ESO-1 antibody failed to experience clinical benefit, we analyzed NY-ESO-1-specific CD4(+) and CD8(+) T-cell responses by intracellular multicytokine staining in 20 NY-ESO-1-seropositive patients and found a surprising dissociation between NY-ESO-1 antibody and CD8 responses in some patients. NY-ESO-1-seropositive patients with associated CD8(+) T cells experienced more frequent clinical benefit (10 of 13; 77%) than those with undetectable CD8(+) T-cell response (one of seven; 14%; P = 0.02; relative risk = 5.4, two-tailed Fisher test), as well as a significant survival advantage (P = 0.01; hazard ratio = 0.2, time-dependent Cox model). Together, our data suggest that integrated NY-ESO-1 immune responses may have predictive value for ipilimumab treatment and argue for prospective studies in patients with established NY-ESO-1 immunity. The current findings provide a strong rationale for the clinical use of modulators of immunosuppression with concurrent approaches to favor tumor antigen-specific immune responses, such as vaccines or adoptive transfer, in patients with cancer.

  7. Expression of NY-ESO-1 and LAGE-1 cancer-testis antigens in hepatocellular carcinoma%NY-ESO-1和LAGE-1癌症-睾丸抗原在肝细胞癌的表达

    Institute of Scientific and Technical Information of China (English)

    张文敏; 肖刚; 张萌; 郭爱林; 董愉; 文剑明

    2005-01-01

    目的检测NY-ESO-1和LAGE-1癌症-睾丸抗原在肝细胞癌中的表达,探讨其作为肝细胞癌免疫治疗靶标的可行性及其与肝癌生物学行为的关系.方法逆转录-聚合酶链反应(RT-PCR)和免疫组织化学EnVision二步法检测30例肝细胞癌新鲜标本NY-ESO-1和LAGE-1的表达;另将191例肝癌石蜡组织制成芯片观察NY-ESO-1蛋白在肝癌中的分布和表达.结果NY-ESO-1和LAGE-1基因mRNA在肝癌中的阳性表达率分别是33.3%(10/30)和16.7%(5/30),至少表达1种基因mRNA者为36.7%(11/30);NY-ESO-1蛋白主要分布在肝癌细胞胞质,有效标本中NY-ESO-1表达13.8%(24/174),小肝癌、中晚期肝癌、发生转移肝癌中的阳性表达率逐渐升高,分别为6.8%(3/44)、16.2%(21/130)、23.1%(12/52),不发生转移的肝癌仅为9.8%(12/122),其中转移组与无转移组之间比较差异有统计学意义(P<0.05),全部病例癌组织与癌旁组织比较差异有统计学意义(P<0.01).NY-ESO-1蛋白的阳性表达与肿瘤大小无关,所有癌旁肝组织均未见NY-ESO-1和LAGE-1的mRNA和蛋白的表达.结论 NY-ESO-1/LAGE-1在肝细胞癌组织中的特异性表达提示其可作为肝细胞癌特异性免疫治疗潜在的靶标;NY-ESO-1在肝癌早期出现,随病情进展表达率逐步增高,转移患者最高,提示NY-ESO-1肽疫苗可能作为晚期肝癌患者治疗手段之一.

  8. Resiquimod as an immunologic adjuvant for NY-ESO-1 protein vaccination in patients with high-risk melanoma.

    Science.gov (United States)

    Sabado, Rachel Lubong; Pavlick, Anna; Gnjatic, Sacha; Cruz, Crystal M; Vengco, Isabelita; Hasan, Farah; Spadaccia, Meredith; Darvishian, Farbod; Chiriboga, Luis; Holman, Rose Marie; Escalon, Juliet; Muren, Caroline; Escano, Crystal; Yepes, Ethel; Sharpe, Dunbar; Vasilakos, John P; Rolnitzsky, Linda; Goldberg, Judith D; Mandeli, John; Adams, Sylvia; Jungbluth, Achim; Pan, Linda; Venhaus, Ralph; Ott, Patrick A; Bhardwaj, Nina

    2015-03-01

    The Toll-like receptor (TLR) 7/8 agonist resiquimod has been used as an immune adjuvant in cancer vaccines. We evaluated the safety and immunogenicity of the cancer testis antigen NY-ESO-1 given in combination with Montanide (Seppic) with or without resiquimod in patients with high-risk melanoma. In part I of the study, patients received 100 μg of full-length NY-ESO-1 protein emulsified in 1.25 mL of Montanide (day 1) followed by topical application of 1,000 mg of 0.2% resiquimod gel on days 1 and 3 (cohort 1) versus days 1, 3, and 5 (cohort 2) of a 21-day cycle. In part II, patients were randomized to receive 100-μg NY-ESO-1 protein plus Montanide (day 1) followed by topical application of placebo gel [(arm A; n = 8) or 1,000 mg of 0.2% resiquimod gel (arm B; n = 12)] using the dosing regimen established in part I. The vaccine regimens were generally well tolerated. NY-ESO-1-specific humoral responses were induced or boosted in all patients, many of whom had high titer antibodies. In part II, 16 of 20 patients in both arms had NY-ESO-1-specific CD4⁺ T-cell responses. CD8⁺ T-cell responses were only seen in 3 of 12 patients in arm B. Patients with TLR7 SNP rs179008 had a greater likelihood of developing NY-ESO-1-specific CD8⁺ responses. In conclusion, NY-ESO-1 protein in combination with Montanide with or without topical resiquimod is safe and induces both antibody and CD4⁺ T-cell responses in the majority of patients; the small proportion of CD8⁺ T-cell responses suggests that the addition of topical resiquimod to Montanide is not sufficient to induce consistent NY-ESO-1-specific CD8⁺ T-cell responses.

  9. AlGaN/GaN High Electron Mobility Transistors with Multi-MgxNy/GaN Buffer

    Directory of Open Access Journals (Sweden)

    P. C. Chang

    2014-01-01

    Full Text Available We report the fabrication of AlGaN/GaN high electron mobility transistors with multi-MgxNy/GaN buffer. Compared with conventional HEMT devices with a low-temperature GaN buffer, smaller gate and source-drain leakage current could be achieved with this new buffer design. Consequently, the electron mobility was larger for the proposed device due to the reduction of defect density and the corresponding improvement of crystalline quality as result of using the multi-MgxNy/GaN buffer.

  10. Antigen-specific in vitro expansion of functional redirected NY-ESO-1-specific human CD8+ T-cells in a cell-free system.

    Science.gov (United States)

    Jakka, Gopinadh; Schuberth, Petra C; Thiel, Markus; Held, Gerhard; Stenner, Frank; Van Den Broek, Maries; Renner, Christoph; Mischo, Axel; Petrausch, Ulf

    2013-10-01

    Tumors can be targeted by the adoptive transfer of chimeric antigen receptor (CAR) redirected T-cells. Antigen-specific expansion protocols are needed to generate large quantities of redirected T-cells. We aimed to establish a protocol to expand functional active NY-ESO-1-specific redirected human CD8(+) T-cells. The anti-idiotypic Fab antibody A4 with specificity for HLA-A 0201/NY-ESO-1157-165 was tested by competition assays using a HLA-A 0201/NY-ESO-1157-165 tetramer. HLA-A 0201/NY-ESO-1157-165 redirected T-cells were generated, expanded and tested for CAR expression, cytokine release, in vitro cytolysis and protection against xenografted HLA-A 0201/NY-ESO-1157-165-positive multiple myeloma cells. A4 demonstrated antigen-specific binding to HLA-A 0201/NY-ESO-1157-165 redirected T-cells. Expansion with A4 resulted in 98% of HLA-A 0201/NY-ESO-1157-165 redirected T-cells. A4 induced strong proliferation, resulting in a 300-fold increase of redirected T-cells. After expansion protocols, redirected T-cells secreted Interleukin-2, (IL-2), interferon gamma (IFNγ) and tumor necrosis factor alpha (TNFα) and lysed target cells in vitro and were protective in vivo. A4 expanded HLA-A 0201/NY-ESO-1157-165 redirected T-cells with preservation of antigen-specific function.

  11. A cancer/testis antigen, NY-SAR-35, induces EpCAM, CD44, and CD133, and activates ERK in HEK293 cells.

    Science.gov (United States)

    Song, Myung-Ha; Kim, Ye-Rin; Bae, Jae-Ho; Shin, Dong-Hoon; Lee, Sang-Yull

    2017-03-04

    The cancer/testis (CT) antigen NY-SAR-35 gene is located on the X chromosome and is aberrantly expressed in various cancers but not in normal tissues, other than testes. Previously, we reported the expression of NY-SAR-35 enhanced cell growth, proliferation, and invasion in HEK293 and cancer cells. To extend understanding of the NY-SAR-35 gene, we used a next generation sequencing (NGS) approach. NY-SAR-35 expression induced growth, proliferation, metastasis, and stemness genes, as indicated by the up-regulations of CXCR4, EpCAM, CD133, and CD44, at the mRNA and protein levels. The expression of NY-SAR-35 in HEK293 cells significantly increased ERK phosphorylation, but not the phosphorylation of AKT. In HEK293/NY-SAR-35 cells, the expressions of pro-apoptotic proteins, including p53, Bax, and p21, were reduced and that of cyclin E was increased. Also, NY-SAR-35 increased the expressions of pluripotency genes (Nanog, Oct-4, and Sox2) and the ability of HEK293 cells to form colonies. Taken together, the present study indicates NY-SAR-35 functions as a CT antigen that triggers oncogenesis and self-renewal.

  12. Allorestricted T lymphocytes with a high avidity T-cell receptor towards NY-ESO-1 have potent anti-tumor activity.

    Science.gov (United States)

    Krönig, Holger; Hofer, Kathrin; Conrad, Heinke; Guilaume, Philippe; Müller, Julia; Schiemann, Matthias; Lennerz, Volker; Cosma, Antonio; Peschel, Christian; Busch, Dirk H; Romero, Pedro; Bernhard, Helga

    2009-08-01

    The cancer-testis antigen NY-ESO-1 has been targeted as a tumor-associated antigen by immunotherapeutical strategies, such as cancer vaccines. The prerequisite for a T-cell-based therapy is the induction of T cells capable of recognizing the NY-ESO-1-expressing tumor cells. In this study, we generated human T lymphocytes directed against the immunodominant NY-ESO-1(157-165) epitope known to be naturally presented with HLA-A*0201. We succeeded to isolate autorestricted and allorestricted T lymphocytes with low, intermediate or high avidity TCRs against the NY-ESO-1 peptide. The avidity of the established CTL populations correlated with their capacity of lysing HLA-A2-positive, NY-ESO-1-expressing tumor cell lines derived from different origins, e.g. melanoma and myeloma. The allorestricted NY-ESO-1-specific T lymphocytes displayed TCRs with the highest avidity and best anti-tumor recognition activity. TCRs derived from allorestricted, NY-ESO-1-specific T cells may be useful reagents for redirecting primary T cells by TCR gene transfer and, therefore, may facilitate the development of adoptive transfer regimens based on TCR-transduced T cells for the treatment of NY-ESO-1-expressing hematological malignancies and solid tumors.

  13. EPA Proposes to Add Dutchess County Creek, N.Y. to the Federal Superfund List, Sediment Contaminated with Mercury, Polycyclic Aromatic Hydrocarbons

    Science.gov (United States)

    (New York, N.Y.) The U.S. Environmental Protection Agency today proposed adding the Wappinger Creek in Dutchess County, N.Y. to its Superfund National Priorities List of the country's most hazardous waste sites. Sediment within the two mile long tidal port

  14. pCDNA3.1/NY-ESO-1真核表达质粒的构建及在肝癌细胞系HepG2中稳定高表达%To construct the eukaryotic expression plasmid pCDNA3.1/NY-ESO-1 and to establish a NY-ESO-1 highly expressed HepG2 cell line

    Institute of Scientific and Technical Information of China (English)

    徐珩; 杨硕; 顾娜; 冯丹丹; 闾军; 汪思应

    2010-01-01

    目的 建立癌-睾丸抗原NY-ESO-1稳定表达的HepG2肝癌细胞系.方法 设计NY-ESO-1的引物,PCR法从NY-ESO-1阳性表达的睾丸癌组织中扩增出目的片段,克隆至T载体,双酶切后定向连入pCDNA3.1载体,构建pCDNA3.1/NY-ESO-1真核表达质粒.经酶切、PCR、测序检测其构建的正确性,脂质体转染法将重组质粒转入HepG2细胞,G418药物筛选出稳定转染的细胞系,RT-PCR法、间接免疫荧光法分别检测稳定转染HepG2细胞中NY-ESO-1基因、蛋白的表达水平.结果 构建的pCDNA3.1/NY-ESO-1质粒经酶切、测序等检验表明质粒构建成功,筛选获得稳定高表达NY-ESO-1的HepG2细胞.结论 构建了pCDNA3.1/NY-ESO-1真核表达质粒及稳定高表达NY-ESO-1的HepG2细胞株,为下一步以NY-ESO-1为靶标进行肝癌的抗原特异性免疫治疗研究奠定了实验基础.

  15. NY-ESO-1抗原的基础与临床研究进展%Current advances in basic and clinical study of NY-ESO-1

    Institute of Scientific and Technical Information of China (English)

    虞淦军

    2015-01-01

    NY-ESO-1是癌-睾丸抗原家族中的重要一员,在多种肿瘤组织中广泛表达,但在正常组织中几乎不表达.目前已经发现了20多个抗原表位,并在多种肿瘤患者体内检测到自发性免疫反应,包括体液和细胞免疫反应,NY-ESO-1的表达及体内自发性免疫应答被证实与肿瘤的诊断和预后有重要关联.NY-ESO-1被认为是肿瘤免疫治疗的理想靶抗原,以NY-ESO-1抗原为靶点的肿瘤治疗性疫苗、TCR基因修饰的T细胞、CAR修饰的T细胞等临床研究相继开展,展示了良好的前景.

  16. From single-site tantalum complexes to nanoparticles of TaxNy and TaOxNy supported on silica: elucidation of synthesis chemistry by dynamic nuclear polarization surface enhanced NMR spectroscopy and X-ray absorption spectroscopy

    KAUST Repository

    Mohandas, Janet Chakkamadathil

    2017-06-08

    Air-stable catalysts consisting of tantalum nitride nanoparticles represented as a mixture of TaxNy and TaOxNy with diameters in the range of 0.5 to 3 nm supported on highly dehydroxylated silica were synthesized from TaMe5 (Me = methyl) and dimeric Ta-2(OMe)(10) with guidance by the principles of surface organometallic chemistry (SOMC). Characterization of the supported precursors and the supported nanoparticles formed from them was carried out by IR, NMR, UV-Vis, extended X-ray absorption fine structure, and X-ray photoelectron spectroscopies complemented with XRD and high-resolution TEM, with dynamic nuclear polarization surface enhanced NMR spectroscopy being especially helpful by providing enhanced intensities of the signals of H-1, C-13, Si-29, and N-15 at their natural abundances. The characterization data provide details of the synthesis chemistry, including evidence of (a) O-2 insertion into Ta-CH3 species on the support and (b) a binuclear to mononuclear transformation of species formed from Ta-2(OMe)(10) on the support. A catalytic test reaction, cyclooctene epoxidation, was used to probe the supported nanoparticles, with 30% H2O2 serving as the oxidant. The catalysts gave selectivities up to 98% for the epoxide at conversions as high as 99% with a 3.4 wt% loading of Ta present as TaxNy/TaOxNy.

  17. 5-Aza-CdR induced expression of NY-ESO-1 antigen in tumor cells%5-Aza-CdR诱导肿瘤细胞NY-ESO-1抗原的表达

    Institute of Scientific and Technical Information of China (English)

    陈裕庆; 黄丽; 汪晓军; 张文敏

    2010-01-01

    目的:探讨甲基化抑制剂5-氮杂-2'-脱氧胞苷(5-Aza-2'-deoxycytidine,5-Aza-CdR)对肿瘤细胞中NY-ESO-1抗原表达的诱导作用.方法:常规培养人胃癌细胞株SGC-7901、人肝癌细胞株H2P和MHCC97-H、人结肠癌细胞株HT-29和LoVo及人脑胶质瘤细胞株U251,RT-PCR和免疫细胞化学法检测5-Aza-CdR作用前后肿瘤细胞中NY-ESO-1 mRNA和蛋白的表达.结果:RT-PCR与免疫细胞化学检测仅见胃癌SGC-7901细胞阳性表达NY-ESO-1,其余5株肿瘤细胞不表达NY-ESO-1.NY-ESO-1阴性的肝癌细胞株H2P、结肠癌细胞株LoVo及脑胶质瘤细胞株U251经5-Aza-CdR(终浓度分别为1、5和10 μmol/L)处理6 d后,细胞形态和生长速度均无明显改变,而NY-ESO-1 mRNA和蛋白均被诱导为阳性表达,并随5-Aza-CdR浓度增加而阳性表达逐渐增强.结论:5-Aza-CdR能诱导肝癌、结肠癌、脑胶质瘤等肿瘤细胞中NY-ESO-1抗原的表达,为肿瘤免疫治疗提供了一条新思路.

  18. Comprehensive adipocytic and neurogenic tissue microarray analysis of NY-ESO-1 expression - a promising immunotherapy target in malignant peripheral nerve sheath tumor and liposarcoma.

    Science.gov (United States)

    Shurell, Elizabeth; Vergara-Lluri, Maria E; Li, Yunfeng; Crompton, Joseph G; Singh, Arun; Bernthal, Nicholas; Wu, Hong; Eilber, Fritz C; Dry, Sarah M

    2016-11-08

    Immunotherapy targeting cancer-testis antigen NY-ESO-1 shows promise for tumors with poor response to chemoradiation. Malignant peripheral nerve sheath tumors (MPNSTs) and liposarcomas (LPS) are chemoresistant and have few effective treatment options. Materials Methods: Using a comprehensive tissue microarray (TMA) of both benign and malignant tumors in primary, recurrent, and metastatic samples, we examined NY-ESO-1 expression in peripheral nerve sheath tumor (PNST) and adipocytic tumors. The PNST TMA included 42 MPNSTs (spontaneous n = 26, NF1-associated n = 16), 35 neurofibromas (spontaneous n = 22, NF-1 associated n = 13), 11 schwannomas, and 18 normal nerves. The LPS TMA included 48 well-differentiated/dedifferentiated (WD/DD) LPS, 13 myxoid/round cell LPS, 3 pleomorphic LPS, 8 lipomas, 1 myelolipoma, and 3 normal adipocytic tissue samples. Stained in triplicate, NY-ESO-1 intensity and density were scored. NY-ESO-1 expression was exclusive to malignant tumors. 100% of myxoid/round cell LPS demonstrated NY-ESO-1 expression, while only 6% of WD/DD LPS showed protein expression, one of which was WD LPS. Of MPNST, 4/26 (15%) spontaneous and 2/16 (12%) NF1-associated MPNSTs demonstrated NY-ESO-1 expression. Strong NY-ESO-1 expression was observed in myxoid/round cell and dedifferentiated LPS, and MPNST in primary, neoadjuvant, and metastatic settings. We found higher prevalence of NY-ESO-1 expression in MPNSTs than previously reported, highlighting a subset of MPNST patients who may benefit from immunotherapy. This study expands our understanding of NY-ESO-1 in WD/DD LPS and is the first demonstration of staining in a WD LPS and metastatic/recurrent myxoid/round cell LPS. These results suggest immunotherapy targeting NY-ESO-1 may benefit patients with aggressive tumors resistant to conventional therapy.

  19. Treatment with 5-Aza-2'-Deoxycytidine Induces Expression of NY-ESO-1 and Facilitates Cytotoxic T Lymphocyte-Mediated Tumor Cell Killing.

    Science.gov (United States)

    Klar, Agnes S; Gopinadh, Jakka; Kleber, Sascha; Wadle, Andreas; Renner, Christoph

    2015-01-01

    NY-ESO-1 belongs to the cancer/testis antigen (CTA) family and represents an attractive target for cancer immunotherapy. Its expression is induced in a variety of solid tumors via DNA demethylation of the promoter of CpG islands. However, NY-ESO-1 expression is usually very low or absent in some tumors such as breast cancer or multiple myeloma. Therefore, we established an optimized in vitro treatment protocol for up-regulation of NY-ESO-1 expression by tumor cells using the hypomethylating agent 5-aza-2'-deoxycytidine (DAC). We demonstrated de novo induction of NY-ESO-1 in MCF7 breast cancer cells and significantly increased expression in U266 multiple myeloma cells. This effect was time- and dose-dependent with the highest expression of NY-ESO-1 mRNA achieved by the incubation of 10 μM DAC for 72 hours. NY-ESO-1 activation was also confirmed at the protein level as shown by Western blot, flow cytometry, and immunofluorescence staining. The detection and quantification of single NY-ESO-1 peptides presented at the tumor cell surface in the context of HLA-A*0201 molecules revealed an increase of 100% and 50% for MCF7 and U266 cells, respectively. Moreover, the enhanced expression of NY-ESO-1 derived peptides at the cell surface was accompanied by an increased specific lysis of MCF7 and U266 cells by HLA-A*0201/NY-ESO-1(157-165) peptide specific chimeric antigen receptor (CAR) CD8+ T cells. In addition, the killing activity of CAR T cells correlated with the secretion of higher IFN-gamma levels. These results indicate that NY-ESO-1 directed immunotherapy with specific CAR T cells might benefit from concomitant DAC treatment.

  20. Treatment with 5-Aza-2'-Deoxycytidine Induces Expression of NY-ESO-1 and Facilitates Cytotoxic T Lymphocyte-Mediated Tumor Cell Killing.

    Directory of Open Access Journals (Sweden)

    Agnes S Klar

    Full Text Available NY-ESO-1 belongs to the cancer/testis antigen (CTA family and represents an attractive target for cancer immunotherapy. Its expression is induced in a variety of solid tumors via DNA demethylation of the promoter of CpG islands. However, NY-ESO-1 expression is usually very low or absent in some tumors such as breast cancer or multiple myeloma. Therefore, we established an optimized in vitro treatment protocol for up-regulation of NY-ESO-1 expression by tumor cells using the hypomethylating agent 5-aza-2'-deoxycytidine (DAC.We demonstrated de novo induction of NY-ESO-1 in MCF7 breast cancer cells and significantly increased expression in U266 multiple myeloma cells. This effect was time- and dose-dependent with the highest expression of NY-ESO-1 mRNA achieved by the incubation of 10 μM DAC for 72 hours. NY-ESO-1 activation was also confirmed at the protein level as shown by Western blot, flow cytometry, and immunofluorescence staining. The detection and quantification of single NY-ESO-1 peptides presented at the tumor cell surface in the context of HLA-A*0201 molecules revealed an increase of 100% and 50% for MCF7 and U266 cells, respectively. Moreover, the enhanced expression of NY-ESO-1 derived peptides at the cell surface was accompanied by an increased specific lysis of MCF7 and U266 cells by HLA-A*0201/NY-ESO-1(157-165 peptide specific chimeric antigen receptor (CAR CD8+ T cells. In addition, the killing activity of CAR T cells correlated with the secretion of higher IFN-gamma levels.These results indicate that NY-ESO-1 directed immunotherapy with specific CAR T cells might benefit from concomitant DAC treatment.

  1. Bliv iværksætter ved at etablere en ny spin-off-virksomhed

    DEFF Research Database (Denmark)

    Clarke, Ann Højbjerg; Evald, Majbritt Rostgaard; Jensen, Kent Wickstrøm;

    2007-01-01

    eller flere iværksættere kan arbejde med spin-off-aktiviteter. Redskabet er opdelt i syv trin. Hvert trin tager fat på konkrete aktiviteter, som erhvervskonsulenten i samarbejde med en iværksætter kan gennemføre for at blive mere bevidst om, hvordan en attraktiv forretningside kan omdannes til en ny...... potentiel spin-off-virksomhed. Denne del af rapporten er oplagt at tage fat på, uanset hvor meget erfaring og viden erhvervskonsulenten har om spin-off-aktiviteter. I indholdsfortegnelsen starter denne del af rapporten med afsnittet "Arbejdsmetoden for de syv trin: Den udførlige model" og ender efter...... gennemgangen af de syv trin. Anden del i rapporten er i indholdsfortegnelsen markeret med en ramme....

  2. Manufacturing of HfOxNy films using reactive magnetron sputtering for ISFET application

    Science.gov (United States)

    Firek, Piotr; Wysokiński, Piotr

    2016-12-01

    Hafnium Oxide-Nitride films were deposited using reactive magnetron sputtering in O2/N2/Ar gas mixture. Deposition was planned according to Taguchi optimization method. Morphology of fabricated layers was tested using AFM technique (Ra=0.2÷1,0 nm). Thickness of HfOXNY films was measured using spectroscopic ellipsometry (t=45÷54 nm). Afterwards MIS structures were created by Al metallization process then layers were electrically characterised using I-V and C-V measurements. This allowed to calculate the electrical parameters of layers such as: flat-band voltage UFB, dielectric constant Ki, interface state trap density Dit and effective charge Qeff. Subsequently, deposited HfOxNy layers were annealed in PDA process (40 min 400 °C 100% N2) after which the electrical characterization was performed again.

  3. Bárány's theory is right, but incomplete. An experimental study in pigeons.

    Science.gov (United States)

    Wit, H P; Spoelstra, H A; Segenhout, J M

    1990-01-01

    The horizontal semicircular canal in pigeons was thermally stimulated with a miniature Peltier-element device. Sound evoked electric responses from the vestibular organ were modulated by the caloric stimulus. Experiments were performed under minimal influence of gravity, (horizontal canals in horizontal plane), and with the pigeons head tilted forwards or backwards. On the basis of the results it was concluded that the influence of gravity can be described by Bárány's convection theory. Extra effects are, however, present and they are most probably partly of mechanical origin (fluid expansion) and partly due to a direct influence of temperature on the vestibular receptors. Further experiments are needed to investigate the origin of these extra effects in more detail.

  4. Polar cloud observatory at Ny-Ålesund in GRENE Arctic Climate Change Research Project

    Science.gov (United States)

    Yamanouchi, Takashi; Takano, Toshiaki; Shiobara, Masataka; Okamoto, Hajime; Koike, Makoto; Ukita, Jinro

    2016-04-01

    Cloud is one of the main processes in the climate system and especially a large feed back agent for Arctic warming amplification (Yoshimori et al., 2014). From this reason, observation of polar cloud has been emphasized and 95 GHz cloud profiling radar in high precision was established at Ny-Ålesund, Svalbard in 2013 as one of the basic infrastructure in the GRENE (Green Network of Excellence Program) Arctic Climate Change Research Project. The radar, "FALCON-A", is a FM-CW (frequency modulated continuous wave) Doppler radar, developed for Arctic use by Chiba University (PI: T. Takano) in 2012, following its prototype, "FALCON-1" which was developed in 2006 (Takano et al., 2010). The specifications of the radar are, central frequency: 94.84 GHz; antenna power: 1 W; observation height: up to 15 km; range resolution: 48 m; beam width: 0.2 degree (15 m at 5 km); Doppler width: 3.2 m/s; time interval: 10 sec, and capable of archiving high sensitivity and high spatial and time resolution. An FM-CW type radar realizes similar sensitivity with much smaller parabolic antennas separated 1.4 m from each other used for transmitting and receiving the wave. Polarized Micro-Pulse Lidar (PMPL, Sigma Space MPL-4B-IDS), which is capable to measure the backscatter and depolarization ratio, has also been deployed to Ny-Ålesund in March 2012, and now operated to perform collocated measurements with FALCON-A. Simultaneous measurement data from collocated PMPL and FALCON-A are available for synergetic analyses of cloud microphysics. Cloud mycrophysics, such as effective radius of ice particles and ice water content, are obtained from the analysis based on algorithm, which is modified for ground-based measurements from Okamoto's retrieval algorithm for satellite based cloud profiling radar and lidar (CloudSat and CALIPSO; Okamoto et al., 2010). Results of two years will be shown in the presentation. Calibration is a point to derive radar reflectivity (dBZ) from original intensity data

  5. Observations of stratospheric ozone above Ny-Ålesund in the Arctic, 2010-2011

    Institute of Scientific and Technical Information of China (English)

    LUO Yuhan; SI Fuqi; LIU Wenqing; SUN Liguang; LIU Yi

    2015-01-01

    Stratosphere ozone depletion above the Arctic region has drawn increased attention recently. Here we present stratospheric ozone column densities above Ny-Ålesund in the Arctic during summer 2010 and 2011, based on a self-developed passive differential optical absorption spectroscopy (DOAS) technique. By analyzing the received scattered solar spectrum, daily variations of ozone vertical column densities (VCDs) were obtained and correlated with satellite-borne ozone monitoring results and ozone sonde data. The comparisons showed good correlation, conifrming the feasibility of DOAS in high-latitude Arctic regions. The preliminary analysis also demonstrated that abnormal low-level ozone columns found in spring 2011 had negative impacts on total ozone column densities over the entire year. The loss of stratospheric ozone may be correlated with low stratospheric temperatures, where heterogeneous atmospheric reactions were active.

  6. QUEST-lærere fra Holstebro får ny naturfags”trailer” i spil

    DEFF Research Database (Denmark)

    Lysgaard, Troels; Sørensen, Allan; Nielsen, Birgitte Lund;

    2015-01-01

    Ungdomsskolen i Holstebro fik mulighed for at udstyre en ny naturfagstrailer til brug for kommunens naturfagslærere. I den forbindelse var det oplagt at anvende netværket fra to år i QUEST projektet. Derfor har naturfagslærere på efteruddannelse i QUEST målsat, designet og afprøvet undervisningsi...

  7. En ny historie om guld og svovl - guld-svovlbindingen i thiolers adsorption på guldnanopartikler og plane guldoverflader

    DEFF Research Database (Denmark)

    Chi, Qijin; Ford, Michael J.; Halder, Arnab;

    2016-01-01

    En netop publiceret større australsk-dansk undersøgelse giver ny indsigt i, hvordan thioldækkede Au-overflader er opbygget. Det har betydning for design, syntese og dannelsesmekanismer for Au-nanopartikler og Au-overflader med skræddersyede fysiske og kemiske egenskaber....

  8. 33 CFR 207.590 - Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Black Rock Canal and Lock at... Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation. (a) The term “canal” when used in this section will mean all of the Black Rock Waterway, including Black Rock Lock, and...

  9. Nano-Crystallization of High-Entropy Amorphous NbTiAlSiWxNy Films Prepared by Magnetron Sputtering

    Directory of Open Access Journals (Sweden)

    Wenjie Sheng

    2016-06-01

    Full Text Available High-entropy amorphous NbTiAlSiWxNy films (x = 0 or 1, i.e., NbTiAlSiNy and NbTiAlSiWNy were prepared by magnetron sputtering method in the atmosphere of a mixture of N2 + Ar (N2 + Ar = 24 standard cubic centimeter per minute (sccm, where N2 = 0, 4, and 8 sccm. All the as-deposited films present amorphous structures, which remain stable at 700 °C for over 24 h. After heat treatment at 1000 °C the films began to crystalize, and while the NbTiAlSiNy films (N2 = 4, 8 sccm exhibit a face-centered cubic (FCC structure, the NbTiAlSiW metallic films show a body-centered cubic (BCC structure and then transit into a FCC structure composed of nanoscaled particles with increasing nitrogen flow rate. The hardness and modulus of the as-deposited NbTiAlSiNy films reach maximum values of 20.5 GPa and 206.8 GPa, respectively. For the as-deposited NbTiAlSiWNy films, both modulus and hardness increased to maximum values of 13.6 GPa and 154.4 GPa, respectively, and then decrease as the N2 flow rate is increased. Both films could be potential candidates for protective coatings at high temperature.

  10. MAGE-A1, GAGE and NY-ESO-1 cancer/testis antigen expression during human gonadal development

    DEFF Research Database (Denmark)

    Gjerstorff, Morten F; Kock, Kirsten; Nielsen, Ole;

    2007-01-01

    by single and double immunohistochemical staining. RESULTS: We found that GAGE was expressed in the primordial germ cells of the gonadal primordium, whereas MAGE-A1 and NY-ESO-1 were first detected in germ cells of both testis and ovary after sexual differentiation was initiated. The number of positive germ...

  11. Expression of MAGE-A3, NY-ESO-1, LAGE-1 and PRAME in urothelial carcinoma

    DEFF Research Database (Denmark)

    Andersen, Lars Dyrskjøt; Zieger, K; Kissow Lildal, T;

    2012-01-01

    Background: The potential for cancer-testis (CT) antigens as targets for immunotherapy in cancer patients has been heavily investigated, and currently cancer vaccine trials based on the CT antigens, MAGE-A3 and NY-ESO-1, are being carried out. Methods: We used specific q-RT-PCR assays to analyse...... the expression of the CT genes MAGE-A3, NY-ESO-1 (CTAG1B), LAGE-1 (CTAG2) and PRAME in a panel of bladder tumours from 350 patients with long-term follow-up and detailed treatment information. Results: Overall, 43% of the tumours expressed MAGE-A3, 35% expressed NY-ESO-1, 27% expressed LAGE-1 and 20% expressed...... PRAME. In all, 56% of the tumours expressed at least one of the CT genes analysed. Univariate Cox regression analysis of CT gene expression in non-muscle-invasive tumours showed that expression of MAGE-A3 (P=0.026), LAGE-1 (P=0.001) and NY-ESO-1 (P=0.040) was significantly associated with a shorter...

  12. Development and Initial Validation of the NyTid Test: A Movement Assessment Tool for Compulsory School Pupils

    Science.gov (United States)

    Tidén, Anna; Lundqvist, Carolina; Nyberg, Marie

    2015-01-01

    This study presents the development process and initial validation of the NyTid test, a process-oriented movement assessment tool for compulsory school pupils. A sample of 1,260 (627 girls and 633 boys; mean age of 14.39) Swedish school children participated in the study. In the first step, exploratory factor analyses (EFAs) were performed in…

  13. MAGE-A is More Highly Expressed Than NY-ESO-1 in a Systematic Immunohistochemical Analysis of 3668 Cases.

    Science.gov (United States)

    Kerkar, Sid P; Wang, Zeng-Feng; Lasota, Jerzy; Park, Tristen; Patel, Krishna; Groh, Eric; Rosenberg, Steven A; Miettinen, Markku M

    2016-05-01

    Two cancer testis antigens, the New York esophageal squamous cell carcinoma-1 (NY-ESO-1) and the melanoma-antigen family A (MAGE-A), represent promising immunotherapy targets due to the low expression of these antigens in nonmalignant tissue. To assess overexpression patterns in various cancers, we performed a systematic immunohistochemical analysis for NY-ESO-1 and MAGE-A on tissue array samples of 3668 common epithelial carcinomas (CA) and germ cell tumors of high prevalence and mortality. Here, we find significantly higher expression of MAGE-A (>50% on tumor cells) compared with NY-ESO-1 in several CAs including cutaneous squamous cell carcinomas (SCC) (52.8%/2.8%), esophageal SCC (50%/0%), head and neck SCC (41.1%/ESO-1, was seen in whole slide evaluations of an independent cohort of metastatic SCC (45.5%/3.6%) and metastatic CA (13.5%/0%) of various primaries with significantly higher expression of MAGE-A in metastatic SCC compared with other metastatic CA. MAGE-A is also more highly expressed in germ cell tumors, seminomas (69%/3.5%) and nonseminomas (40.1%/4.7%). In summary, MAGE-A is more highly expressed than NY-ESO-1 in a majority of human malignancies, and targeting MAGE-A may benefit a large number of patients.

  14. Implementering af ny standard for kateterisering i forhold til ældre svagelige patienter > 65 år med hoftefraktur

    DEFF Research Database (Denmark)

    Bagger, Bettan; Varmarken, Tine; Pedersen, Niels

    2013-01-01

    klinisk standard. Metode: Der er udarbejdet et systematisk review med henblik på at identificere best evidence i forhold til brug af intermitterende versus permanent kateterisation. Der er implementeret en ny evidensbaseret standard i afdelingen, som evaluereres gennem en efterfølgende audit og...

  15. 76 FR 66685 - Foreign-Trade Zone 37-Orange County, NY, Application for Subzone, ITT Water Technology, Inc...

    Science.gov (United States)

    2011-10-27

    ... Technology, Inc. (Centrifugal and Submersible Pumps), Auburn, NY An application has been submitted to the... special purpose subzone status for the centrifugal and submersible pump manufacturing and warehousing... facilities are used to manufacture and distribute centrifugal and submersible pumps and related...

  16. 33 CFR 100.121 - Swim Across the Sound, Long Island Sound, Port Jefferson, NY to Captain's Cove Seaport...

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Swim Across the Sound, Long... SAFETY OF LIFE ON NAVIGABLE WATERS § 100.121 Swim Across the Sound, Long Island Sound, Port Jefferson, NY... they are officially participating in the Swim Across the Sound event or are otherwise authorized by...

  17. 77 FR 46377 - Foreign-Trade Zone 37-Orange County, NY, Notification of Proposed Production Activity, Takasago...

    Science.gov (United States)

    2012-08-03

    ..., mixtures of odiferous substances and essential oils (duty rate ranges from duty free to 7%). Public comment... Foreign-Trade Zones Board Foreign-Trade Zone 37--Orange County, NY, Notification of Proposed Production... (Takasago) submitted a notification of proposed production activity for their facility located in...

  18. Cancer-testis antigens PRAME and NY-ESO-1 correlate with tumour grade and poor prognosis in myxoid liposarcoma.

    Science.gov (United States)

    Iura, Kunio; Kohashi, Kenichi; Hotokebuchi, Yuka; Ishii, Takeaki; Maekawa, Akira; Yamada, Yuichi; Yamamoto, Hidetaka; Iwamoto, Yukihide; Oda, Yoshinao

    2015-07-01

    Myxoid liposarcoma is the second most common liposarcoma. Although myxoid liposarcoma is relatively chemosensitive and thus a good candidate for chemotherapy, cases with relapsed or metastatic disease still have poor outcome. Here, we performed a gene microarray analysis to compare the gene expression profiles in six clinical myxoid liposarcoma samples and three normal adipose tissue samples, and to identify molecular biomarkers that would be useful as diagnostic markers or treatment targets in myxoid liposarcoma. This showed that the cancer-testis antigen PRAME was up-regulated in myxoid liposarcoma. We then performed immunohistochemical, western blotting and real-time polymerase chain reaction analyses to quantify the expression of PRAME and another cancer-testis antigen, NY-ESO-1, in clinical samples of myxoid liposarcoma (n = 93), dedifferentiated (n = 46), well-differentiated (n = 32) and pleomorphic liposarcomas (n = 14). Immunohistochemically, positivity for PRAME and NY-ESO-1 was observed in 84/93 (90%) and 83/93 (89%) of the myxoid liposarcomas, and in 20/46 (43%) and 3/46 (7%) of the dedifferentiated, 3/32 (9%) and 1/32 (3%) of the well-differentiated and 7/14 (50%) and 3/21 (21%) of the pleomorphic liposarcomas, respectively. High immunohistochemical expression of PRAME and/or NY-ESO-1 was significantly correlated with tumour diameter, the existence of tumour necrosis, a round-cell component of >5%, higher histological grade and advanced clinical stage. High PRAME and NY-ESO-1 expression correlated significantly with poor prognosis in a univariate analysis. The myxoid liposarcomas showed significantly higher protein and mRNA expression levels of PRAME and NY-ESO-1 (CTAG1B) than the other liposarcomas. In conclusion, PRAME and NY-ESO-1 (CTAG1B) were expressed in the vast majority of myxoid liposarcomas, and their high-level expression correlated with tumour grade and poor prognosis. Our results support the potential use of PRAME and NY

  19. [Activities of Psychology Dept., California Univ.

    Science.gov (United States)

    Bridgeman, Bruce

    1998-01-01

    We have completed two studies during the grant period, with manuscripts published or ready for submission for publication: (1) Dual adaptation and adaptive generalization in the human vestibuloocular reflex and (2) Frequency vs. acceleration specificity in human VOR adaptation. In the 1st study two studies examined the possibility that rotational VOR plasticity is subject to dual adaptation and adaptive generalization. Subjects in the experimental condition were exposed to an altered visual-vestibular environment for about four minutes every day for five consecutive days. The working hours between these testing sessions constituted re-exposure to the normal visual environment. Thus, subjects were repeatedly adapting and re-adapting to both environments which is a condition designed to produce dual adaptation. In each training session a measure of baseline VOR gain was obtained (in the dark). A small laser spot (the only visual stimulus) was systematically moved in the same direction as the subject's head, but by half the angle of rotation (target/head gain = 0.5). This resulted in adaptation values relativized to the non-adapted gain of each subject. These values were then analyzed using an analysis of variance with day and session (within a day) as factors. In the 2nd study human VOR adaption has been assumed to be frequency specific, despite the fact that the semicircular canals are simulated by rotational acceleration and not frequency per se.

  20. Immunoediting and persistence of antigen-specific immunity in patients who have previously been vaccinated with NY-ESO-1 protein formulated in ISCOMATRIX™.

    Science.gov (United States)

    Nicholaou, Theo; Chen, Weisan; Davis, Ian D; Jackson, Heather M; Dimopoulos, Nektaria; Barrow, Catherine; Browning, Judy; Macgregor, Duncan; Williams, David; Hopkins, Wendie; Maraskovsky, Eugene; Venhaus, Ralph; Pan, Linda; Hoffman, Eric W; Old, Lloyd J; Cebon, Jonathan

    2011-11-01

    NY-ESO-1 protein formulated in ISCOMATRIX™ results in CD4+, CD8+ T cell and antibody-mediated immunity. We evaluated persistence of immunity, relapse-free survival and tumour antigen expression upon relapse in patients vaccinated in an earlier trial. Immunity was measured in 28 patients with resected NY-ESO-1-expressing tumours (melanoma 25, breast 3) 252-1,155 days (median = 681) after vaccination. In the earlier vaccination, trial patients received NY-ESO-1 with ISCOMATRIX™ adjuvant at three protein doses 10 μg, 30 μg or 100 μg (n = 14); 100 μg NY-ESO-1 protein (n = 8) or placebo (n = 6), together with 1 μg of intradermal (ID) NY-ESO-1 protein twice for DTH skin testing. Immune responses assessed in the current study included antibody titres, circulating NY-ESO-1-specific T cells and DTH reactivity 2 days after DTH skin testing with NY-ESO-1 protein (1 μg) or peptides (10 μg). Relapse-free survival was determined for 42 melanoma patients. On relapse NY-ESO-1 and HLA, class I was assessed by immunohistochemistry in 17. Persisting anti-NY-ESO-1 immunity was detected in 10/14 recipients who had previously received vaccine with ISCOMATRIX™ adjuvant. In contrast, immunity only persisted in 3/14 who received 100 μg un-adjuvanted NY-ESO-1 protein (3/8) or 2 μg DTH protein (0/6) P = 0.02. Hence, persisting NY-ESO-1 immunity was associated with prior adjuvant. Tumour NY-ESO-1 or HLA class I was downregulated in participants who relapsed suggesting immunoediting had occurred. Immunoediting suggests that a signal of anti-tumour activity was observed in high-risk resected melanoma patients vaccinated with NY-ESO-1/ISCOMATRIX™. This was associated with measurable persisting immunity in the majority of vaccinated subjects tested. A prospective randomised trial has been undertaken to confirm these results.

  1. Fine-mapping naturally occurring NY-ESO-1 antibody epitopes in melanoma patients' sera using short overlapping peptides and full-length recombinant protein.

    Science.gov (United States)

    Komatsu, Nobukazu; Jackson, Heather M; Chan, Kok-fei; Oveissi, Sara; Cebon, Jonathan; Itoh, Kyogo; Chen, Weisan

    2013-07-01

    The tumor antigen NY-ESO-1 is one of the most antigenic cancer-testis antigens, first identified by serologic analysis of a recombinant cDNA expression library (SEREX). NY-ESO-1 is expressed in different types of cancers including melanoma. NY-ESO-1-specific spontaneous humoral and cellular immune responses are detected in a large proportion of patients with advanced NY-ESO-1-expressing cancers. Therefore NY-ESO-1 is a good candidate antigen for immunotherapy. Although cellular immune responses to NY-ESO-1 are well characterized, much less is known about the humoral immune responses. In this study, we finely mapped linear antibody epitopes using sera from melanoma patients and shorter overlapping peptide sets. We have shown that melanoma patients' humoral immune systems responded to NY-ESO-1 differently in each individual with widely differing antibody specificity, intensity and antibody subtypes. This knowledge will help us further understand anti-tumor immunity and may also help us to monitor cancer progress and cancer vaccine efficacy in the future. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Expression of NY-ESO-1 in Triple-Negative Breast Cancer Is Associated with Tumor-Infiltrating Lymphocytes and a Good Prognosis.

    Science.gov (United States)

    Lee, Hee Jin; Kim, Joo Young; Song, In Hye; Park, In Ah; Yu, Jong Han; Gong, Gyungyub

    2015-01-01

    Accumulating evidence suggests that immunotherapy has great potential for treating triple-negative breast cancer (TNBC). We analyzed the expression of NY-ESO-1, which is a potent immunogenic cancer testis antigen, and its association with clinicopathological factors in large cohorts of breast cancer patients. A total of 623 consecutive breast cancer patients who underwent surgery between 1993 and 1998 and 612 TNBC patients who underwent surgery between 2004 and 2010 at Asan Medical Center were included. Immunohistochemical staining for NY-ESO-1 was performed using tissue microarrays. NY-ESO-1 was expressed in 2.6% of consecutive breast cancers, all of which were TNBC (p ESO-1 expression was identified in 9.7% of the TNBC cohort and was significantly correlated with a higher level of tumor-infiltrating lymphocytes (TIL; p = 0.026). In survival analyses, a lower level of TIL (all, p ESO-1 expression (p = 0.024) were significantly associated with poor disease-free survival. Additionally, positive NY-ESO-1 expression was an independent favorable prognostic factor in TNBC patients (p = 0.046). NY-ESO-1 is specifically expressed in TNBC, and NY-ESO-1 expression is an independent good prognostic factor in TNBC. Evaluation of NY-ESO-1 expression in TNBC might be useful for selecting patients who may benefit from vaccination therapy and also has a prognostic significance in TNBC. © 2015 S. Karger AG, Basel.

  3. Persistent deNOx Ability of CaAl2O4:(Eu, Nd/TiO2-xNy Luminescent Photocatalyst

    Directory of Open Access Journals (Sweden)

    Li Huihui

    2011-01-01

    Full Text Available Abstract CaAl2O4:(Eu, Nd/TiO2-xNy composite luminescent photocatalyst was successfully synthesized by a simple planetary ball milling process. Improvement of photocatalytic deNOx ability of TiO2-xNy, together with the persistent photocatalytic activity for the decomposition of NO after turning off the light were realized, by coupling TiO2-xNy with long afterglow phosphor, CaAl2O4:(Eu, Nd. The novel persistent photocatalytic behavior was related to the overlap between the absorption wavelength of TiO2-xNy and the emission wavelength of the CaAl2O4:(Eu, Nd. It was found that CaAl2O4:(Eu, Nd/TiO2-xNy composites provided the luminescence to persist photocatalytic reaction for more than 3 h after turning off the light. Graphical Abstract CaAl2O4:(Eu, Nd/TiO2-xNy composite luminescent photocatalyst with persistent deNOx activity after turning off the light was successfully synthesized by a simple planetary ball milling process. The novel persistent photocatalytic behavior was related to the overlap between the absorption wavelength of TiO2-xNy and the emission wavelength of the CaAl2O4:(Eu, Nd. Additional file 1 Click here for file

  4. The cancer-testis antigen NY-ESO-1 is highly expressed in myxoid and round cell subset of liposarcomas.

    Science.gov (United States)

    Hemminger, Jessica A; Ewart Toland, Amanda; Scharschmidt, Thomas J; Mayerson, Joel L; Kraybill, William G; Guttridge, Denis C; Iwenofu, O Hans

    2013-02-01

    Liposarcomas are a heterogenous group of fat-derived sarcomas, and surgery with or without chemoradiation therapy remains the main stay of treatment. NY-ESO-1 is a cancer-testis antigen expressed in various cancers where it can induce both cellular and humoral immunity. Immunotherapy has shown promise in clinical trials involving NY-ESO-1-expressing tumors. Gene expression studies have shown upregulation of the gene for NY-ESO-1, CTAG1B, in myxoid and round cell liposarcomas. Herein, we evaluated the expression of NY-ESO-1 among liposarcoma subtypes by quantitative real-time PCR, western blot analysis, and immunohistochemistry. Frozen tissue for quantitative real-time PCR and western blot analysis was obtained for the following liposarcoma subtypes (n=15): myxoid and round cell (n=8); well-differentiated (n=4), and dedifferentiated (n=3). Formalin-fixed paraffin-embedded blocks were obtained for the following liposarcoma subtypes (n=44): myxoid and round cell (n=18); well-differentiated (n=10); dedifferentiated (n=10); and pleomorphic (n=6). Full sections were stained with monoclonal antibody NY-ESO-1, and staining was assessed for intensity (1-3+), percentage of tumor positivity, and location. In all, 7/8 (88%) and 16/18 (89%) myxoid and round cell expressed CTAG1B and NY-ESO-1 by quantitative real-time PCR and immunohistochemistry, respectively. Western blot correlated with mRNA expression levels. By immunohistochemistry, 94% (15/16) of positive cases stained homogenously with 2-3+ intensity. Also, 3/6 (50%) pleomorphic liposarcomas demonstrated a range of staining: 1+ intensity in 50% of cells; 2+ intensity in 5% of cells; and 3+ intensity in 90% of cells. One case of dedifferentiated liposarcoma showed strong, diffuse staining (3+ intensity in 75% of cells). Our study shows that both CTAG1B mRNA and protein are overexpressed with high frequency in myxoid and round cell liposarcoma, enabling the potential use of targeted immunotherapy in the treatment of this

  5. Expression of NY-ESO-1 gene detected by RT-PCR in human gastric carcinoma and its gene cloning%RT-PCR检测胃癌NY-ESO-1抗原的表达及其基因克隆

    Institute of Scientific and Technical Information of China (English)

    黄梅花; 刘磊; 欧阳永忠

    2005-01-01

    目的研究NY-ESO-1基因在胃癌中的表达,以便了解胃癌患者是否可以使用NY-ESO-1抗原为基础的疫苗治疗.方法采用RT-PCR方法检测NY-ESO-1基因在60例胃癌组织和相应的癌旁正常组织中的表达,采用分子克隆的方法从胃癌组织中克隆了NY-ESO-1 cDNA.结果 NY-ESO-1基因在胃癌组织中的表达率为55%(33/60),在相应的癌旁正常组织中未见表达,克隆的NY-ESO-1cDNA序列与GenBank报道一致.结论 NY-ESO-1基因在胃癌中呈高表达率,NY-ESO-1抗原可以被具有HLA-1类分子限制性CTL识别.

  6. RT-PCR检测胃癌NY-ESO-1抗原的表达及其基因克隆%Expression of NY-ESO-1 gene was analyzed by RT-PCR in human gastric carcinoma and its cloning

    Institute of Scientific and Technical Information of China (English)

    黄梅花; 刘磊; 欧阳永忠

    2005-01-01

    目的:研究NY-ESO-1基因在胃癌中的表达,以便了解为胃癌患者是否可以使用NY-ESO-1抗原为基础的疫苗治疗.方法:采用RT-PCR方法检测了NY-ESO-1基因在60例胃癌组织和相应的癌旁正常组织中的表达,采用分子克隆的方法从胃癌组织中克隆了NY-ESO-1cDNA.结果:NY-ESO-1基因在胃癌组织中的表达率为55%(33/60),在相应的癌旁正常组织中的未见表达,克隆的NY-ESO-1cDNA序列与GenBank报道一致.结论:NY-ESO-1基因在胃癌中的高表达率,NY-ESO-1抗原可以被具有HLA-1类分子限制性CTL识别.

  7. On the spin modulated circular polarization from the intermediate polars NY Lup and IGRJ1509-6649

    CERN Document Server

    Potter, Stephen B; Kotze, Marissa; Zietsman, Ewald; Butters, O W; Pekeur, Nikki; Buckley, David A H

    2011-01-01

    We report on high time resolution, high signal/noise, photo-polarimetry of the intermediate polars NY Lup and IGRJ1509-6649. Our observations confirm the detection and colour dependence of circular polarization from NY Lup and additionally show a clear white dwarf, spin modulated signal. From our new high signal/noise photometry we have unambiguously detected wavelength dependent spin and beat periods and harmonics thereof. IGRJ1509-6649 is discovered to also have a particularly strong spin modulated circularly polarized signal. It appears double peaked through the I filter and single peaked through the B filter, consistent with cyclotron emission from a white dwarf with a relatively strong magnetic field. We discuss the implied accretion geometries in these two systems and any bearing this may have on the possible relationship with the connection between polars and soft X-ray-emitting IPs. The relatively strong magnetic fields is also suggestive of them being polar progenitors.

  8. Ansatte perspektiver på utvikling av tverrfaglighet i ny sykehusavdeling

    Directory of Open Access Journals (Sweden)

    Hildfrid V. Brataas

    2009-02-01

    Full Text Available Artikkelen omhandler en følgestudie om ansattes perspektiver på teamorganisert og brukerinvolvert tverrfaglig samarbeid i en ny rehabiliteringsavdeling i et norsk sykehus. Studiens formål var å bringe frem mer kunnskap om forhold av betydning ved utvikling av tverrfaglige arbeidsformer i sammensatte helsetjenester.Metode: Undersøkelsen var kvalitativt beskrivende og fortolkende med bruk av fokusgruppeintervjuer tre og fem år etter oppstart av rehabiliteringsavdelingen.Resultater: Over en femårsperiode var praksis erfart å endre karakter, fra flerfaglig samarbeid til funksjonelt tverrfaglig samarbeid. Profesjonsoverbyggende teori om rehabiliterings-virksomheten og ledelsens kvalitetsorientering var erfart som hjelp for teamenes utvikling av funksjonell tverrfaglighet.Konklusjon: Langsiktighet, kvalitetsorientert ledelse og teamorganisert rehabiliteringsarbeid basert på profesjonsoverbyggende teoriramme synes å underbygge utvikling av funksjonell tverrfaglighetsforståelse og praksis. Denne kunnskapen er relevant ved omlegging til teamorganisert rehabiliteringsvirksomhet i eksisterende og nye virksomheter. Det er behov for mer forskning om betydning av personfaktorer og motivasjon, ledelse og overbyggende teorirammeverk for samarbeidsutvikling og kvalitet i praksis.

  9. Integrated water vapor above Ny Ålesund, Spitsbergen: a multi-sensor intercomparison

    Directory of Open Access Journals (Sweden)

    M. Pałm

    2010-02-01

    Full Text Available Water vapor is an important constituent of the atmosphere. Because of its abundance and its radiative properties it plays an important role for the radiation budget of the atmosphere and has major influence on weather and climate.

    In this work integrated water vapor (IWV derived from the measurements of three satellite sensors, GOME, SCIAMACHY and AMSU-B, two ground based sensors, a Fourier-transform spectrometer (FTIR, a microwave radiometer for O3 (RAM and IWV inferred from GPS zenith path delay (ZPD measurements, are compared to radio-sonde measurements above Ny Ålesund, 79° N. All six remote sensors exploit different principles and work in different wavelength regions.

    All remote sensing instruments reproduce the sonde measurements very well and are highly correlated when compared with the radio-sonde measurements.

    The ground-based FTIR shows very little scatter of about 10%. The GPS performs similar to the FTIR at all times except for very low IWV, where the scatter exceeds 50% of the measured IWV. The other remote sensing instruments show scatter of about 20% (standard deviation. The ground-based RAM performs similar to the satellite instruments, despite the fact that the retrieval of IWV is just a by-product of this ozone sensor.

  10. Characterization of a Single Magnetotactic Bacterial Species from Devil's Bathtub, Mendon Ponds Park, Honeoye Falls, NY

    Science.gov (United States)

    Wagner, C.; Tarduno, J. A.; Stein, A.; Sia, E.

    2015-12-01

    Magnetotactic bacteria (MTB) belong to a lineage of prokaryotic bacteria that synthesize magnetosomes, single domain magnetic particles (typically magnetite or greigite) with an average size of 50 nanometers. MTB utilize magnetosomes through magnetotaxis, the alignment and movement along magnetic field lines to navigate towards preferred environmental conditions. MTB are sensitive to different environments and are thought to exhibit varying magnetosome morphologies, compositions, sizes, and quantities in regards to the environments which they inhabit. These characteristics allow MTB and magnetofossils (preserved magnetosomes) to be used as modern/paleoenvironmental recorders and biomarkers for environmental change(s). Devil's Bathtub (Mendon Ponds Park, Honeoye Falls, NY) is a meromictic glacial kettle pond surrounded by deciduous tree cover. Here we examine one species of MTB based on prominence of this particular morphology at this locale. Magnetotaxis and morphology of this species have been observed using light microscopy. Micrographs have also been taken using Transmission Electron Microscopy (TEM) to verify cell morphology and to determine magnetosome morphology. TEM and magnetic hysteresis measurements were done to find and test the composition of magnetosomes. In this study we also focus on DNA sequencing and characterization of this MTB, as there are few MTB species which have been DNA sequenced successfully. Data from these experiments are directly applicable to this up-and-coming area of research as it will aid in the understanding and correlation of magnetosome and magnetofossils with environmental characteristics.

  11. Single-particle characterization of summertime arctic aerosols collected at Ny-Alesund, Svalbard.

    Science.gov (United States)

    Geng, Hong; Ryu, Jiyeon; Jung, Hae-Jin; Chung, Hyeok; Ahn, Kang-Ho; Ro, Chul-Un

    2010-04-01

    Single-particle characterization of summertime Arctic aerosols is useful to understand the impact of air pollutants on the polar atmosphere. In the present study, a quantitative single particle analytical technique, low-Z particle electron probe X-ray microanalysis, was used to characterize 8100 individual particles overall in 16 sets of aerosol samples collected at Ny-Alesund, Svalbard, Norway on 25-31 July, 2007. Based on their X-ray spectral and secondary electron image data of individual particles, 13 particle types were identified, in which particles of marine origin were the most abundant, followed by carbonaceous and mineral dust particles. A number of aged (reacted) sea salt (and mixture) particles produced by the atmospheric reaction of genuine sea-salts, especially with NO(x) or HNO(3), were significantly encountered in almost all the aerosol samples. They greatly outnumbered genuine sea salt particles, implying that the summertime Arctic atmosphere, generally regarded as a clean background environment, is disturbed by anthropogenic air pollutants. The main sources of airborne NO(x) (or HNO(3)) are probably ship emissions around the Arctic Ocean, industry emission from northern Europe and northwestern Siberia, and renoxification of NO(3)(-) within or on the melting snow/ice surface.

  12. Assessment and Molecular Characterization of Human Intestinal Parasites in Bivalves from Orchard Beach, NY, USA.

    Science.gov (United States)

    Tei, Freda F; Kowalyk, Steven; Reid, Jhenelle A; Presta, Matthew A; Yesudas, Rekha; Mayer, D C Ghislaine

    2016-03-29

    Bivalves have been shown to be carriers of the human intestinal parasites Cryptosporidium parvum and Toxoplasma gondii. The goal of this study is to determine the prevalence of protozoan parasites in mollusks of New York City using a polymerase chain reaction (PCR)-based assay. Four species of mollusks, Mya arenaria, Geukensia demissa, Crassostrea virginica, and Mytilis edulis, were collected from Orchard Beach, NY in the fall of 2014, totaling 159 specimens. Each individual mollusk was dissected to harvest the digestive gland, the mantle, the gills, the foot and the siphon. The tissues were assayed for the presence of Cryptosporidium parvum, Giardia lamblia, and Toxoplasma gondii DNA by using primers that target parasite-specific genes. C. parvum was found at a prevalence of 50%, 11.3%, and 1%, respectively, in Mya arenaria, G. demissa, and Mytilis edulis. C. parvum DNA was detected in all the tissues of these bivalve species, except the gills. Furthermore, G. lamblia was detected in Mya arenaria, G. demissa, Crassostrea virginica and Mytilis edulis at a prevalence of 37.5%, 4.5%, 60%, and 20.6%, respectively, while T. gondii DNA was not detected.

  13. Local Volume TiNy Titans: Gaseous Dwarf-Dwarf Interactions in the Local Universe

    CERN Document Server

    Pearson, Sarah; Putman, Mary E; Lutz, Katharina A; Fernandez, Ximena; Stierwalt, Sabrina; Patton, David R; Kim, Jinhyub; Kallivayalil, Nitya; Johnson, Kelsey; Sung, Eon-Chang

    2016-01-01

    In this paper, we introduce the Local Volume TiNy Titans sample (LV-TNT), which is a part of a larger body of work on interacting dwarf galaxies: TNT (Stierwalt et al. 2015). This LV-TNT sample consists of 10 dwarf galaxy pairs in the Local Universe (< 30 Mpc from Milky Way), which span mass ratios of M_(*,1)/M_(*,2) < 20, projected separations < 100 kpc, and pair member masses of log(M_*/M_Sun) < 9.9. All 10 LV-TNT pairs have resolved synthesis maps of their neutral hydrogen, are located in a range of environments and captured at various interaction stages. This enables us to do a comparative study of the diffuse gas in dwarf-dwarf interactions and disentangle the gas lost due to interactions with halos of massive galaxies, from the gas lost due to mutual interaction between the dwarfs. We find that the neutral gas is extended in the interacting pairs when compared to non-paired analogs, indicating that gas is tidally pre-processed. Additionally, we find that the environment can shape the HI dist...

  14. Determination of Conduction and Valence Band Electronic Structure of LaTiOx Ny Thin Film.

    Science.gov (United States)

    Pichler, Markus; Szlachetko, Jakub; Castelli, Ivano E; Marzari, Nicola; Döbeli, Max; Wokaun, Alexander; Pergolesi, Daniele; Lippert, Thomas

    2017-05-09

    The nitrogen substitution into the oxygen sites of several oxide materials leads to a reduction of the band gap to the visible-light energy range, which makes these oxynitride semiconductors potential photocatalysts for efficient solar water splitting. Oxynitrides typically show a different crystal structure compared to the pristine oxide material. As the band gap is correlated to both the chemical composition and the crystal structure, it is not trivial to distinguish which modifications of the electronic structure induced by the nitrogen substitution are related to compositional and/or structural effects. Here, X-ray emission and absorption spectroscopy are used to investigate the electronic structures of orthorhombic perovskite LaTiOx Ny thin films in comparison with films of the pristine oxide LaTiOx with similar orthorhombic structure and cationic oxidation state. Experiment and theory show the expected upward shift in energy of the valence band maximum that reduces the band gap as a consequence of the nitrogen incorporation. This study also shows that the conduction band minimum, typically considered almost unaffected by nitrogen substitution, undergoes a significant downward shift in energy. For a rational design of oxynitride photocatalysts, the observed changes of both the unoccupied and occupied electronic states have to be taken into account to justify the total band-gap narrowing induced by the nitrogen incorporation. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Aromaticity of Heterofullerenes C18BxNy (x+y=2) and Their Molecular Ions

    Institute of Scientific and Technical Information of China (English)

    Jia-li Chen; Ablikim Kerim

    2008-01-01

    The axomaticity of all possible substituted fullerene isomers of C18N2,C18N2,C18BN,and their molecular ions which originate from the C20 (Ih) cage were studied by the topological resonance energy (TRE) and the percentage topological resonance energy methods.The relationship between the aromaticity of C18BxNy isomers and the sites where the heteroatoms dope at the C20 (Ih) cage is discussed.Calculation results show that at the neutral and cationic states all the isomers axe predicted to be antiaromatic with negative TREs,but their polyvalent anions are predicted to be aromatic with positive TREs.The most stable isomer is formed by heteroatom doping at the 1,11-sites in C18N2,C18B2,and C18BN.Heterofullerenes are more aromatic than C20.The stability order in the neutral states is C18N2>C18BN>C18B2>C20.The stability order in closed-shell is C18B28->C206->C18BN6->C18N24-.This predicts theoretically that their polyvalent anions have high aromaticity.

  16. Everyday Physical Activity of Students in Nyíregyháza

    Directory of Open Access Journals (Sweden)

    Fintor János Gábor

    2015-05-01

    Full Text Available The popularity of physical education lessons has already been demonstrated by a lot of essays, however, it has also been revealed that this popularity, as well as the frequency of doing sports, tends to decrease at later ages of life. Pursuing sports has a positive effect on academic performance. Introducing PE as an everyday lesson at schools was a milestone in physical education. Provided the aims are realised successfully, the general state of health of students can show a favourable change. The aim of our research, on the one hand, is to reveal how important physical education is as contrasted with other school subjects, according to those students asked. On the other hand, we aim to examine how the certain positive and negative attitudes of the subjects of our study appear in the resulting figures. The research was conducted in the form of self-completion questionnaires in four different funded institutions in Nyíregyháza (n=285, in May, 2014. In the course of evaluation, we have used multivariate function analysis as well as frequency research and cross table reference. Participants mainly regard PE as important as other school subjects, however, boys significantly regard it more important than girls do. Those who do sports on a regular basis were more likely to agree that PE lessons are conducted in a friendly atmosphere than those who do sports less. Significantly, boys tended to agree with positive statements about sport.

  17. Rugby-metoden SCRUM: En ny måde at bedrive projekter på

    DEFF Research Database (Denmark)

    Pries-Heje, Lene; Pries-Heje, Jan

    2012-01-01

    En ny måde at bedrive projekter på breder sig i disse år med overraskende fart. Selv om de første tanker om ”Scrum” – hvor navnet og fremgangsmåden er inspireret af Rugby-spillet – blev tænkt for mere end 10 år siden, så er det først nu at fremgangsmåden for alvor har vundet udbredelse i Danmark....... Denne artikel gennemgår metoden og fortæller om både positive resultater og udfordringer i tre danske organisationer: Et softwarehus, en bank, og et universitet. De tre cases illustrer meget forskellige anvendelsessituationer af Scrum set i forhold til projekternes størrelse, karakter og den...... organisatoriske kontekst. Artiklen slutter med at diskutere, hvad det er der gør, at Scrum er blevet så populær, og i dag fremstår som et reelt alternativ til klassisk plandreven projektledelse...

  18. Hudson River Paleoclimate, Sea Level, and Human Impact: A Record From Piermont Marsh, NY

    Science.gov (United States)

    Kurdyla; Peteet, Dorothy; Liberman, Louisa; Sugar; Wong; Hansen, James E. (Technical Monitor)

    2001-01-01

    A 13.77 meter sediment core from Piermont Marsh, NY (40 00 N, 73 55W) records the local and regional vegetational and foraminiferal history of the Hudson Estuary. The sediments were sampled every 4 cm, which represents a decadal to centuryscale resolution. Basal sediment dating is in progress, and the 11-m depth represents about 4000 years. Changes in plant macrofossils and charcoal appear to indicate differences in salinIty and drought, suggesting changes in climate. Scirpus, Salicornia, and high levels of charcoal seem to indicate drier/more saline conditions, while lack of these macrofossils and increases in Chara/Nitella, aquatic leaves, and very little charcoal suggests wetter conditions. Other macrofossils include Carex, Juncus, Polygonum, Zanichellia, Ruppia. High resolution AMS dating of plant macrofossils is in progress, and will be compared with changes in Hudson River sediment cores offshore. Foraminiferal assemblages from key intervals of the core will be presented. Human impact in the upper sediments is visible from the influx of grass seeds, primarily Phragmites, and the ragweed pollen rise.

  19. Water residence times and nutrient budgets across an urbanizing gradient (Croton water supply area, NY)

    Science.gov (United States)

    Vitvar, T.; Burns, D. A.; Duncan, J. M.; Hassett, J. M.; Mitchell, M. J.

    2002-05-01

    Water residence times and nutrient budgets in 3 small watersheds in the Croton water supply area, NY, were examined. The watersheds (less than 1km 2) have different level of urbanization (natural, semi-developed and fully developed), different mechanisms of runoff generation (quick flow on roads and slow flow through subsurface) and different watershed landscape characteristics (wet zones, hillslopes) . Measurements of the comprehensive chemical suite incl. components of nitrogen budget in the throughfall, stream water, soil water and groundwater in the saturated zone were performed bi-weekly over a period up to 2 years. Mean water residence times of the stream water were estimated using Oxygen-18 and Helium-3/Tritium isotopes. There are significant differences in the chemical composition and decrease of nitrification intensity and of mean streamwater residence time along the increasing watershed development. Within each watershed, longer water residence times (up to over 2 years) were estimated in the wetland zones without direct communication with streams in comparison to hillslope areas (up to over 1 year). The results can be used in watershed management and planning of the further urbanization of this water supply area.

  20. Aromaticity of Heterofullerenes C18BxNy (x + y = 2) and Their Molecular Ions

    Science.gov (United States)

    Chen, Jia-li; Kerim, Ablikim

    2008-04-01

    The aromaticity of all possible substituted fullerene isomers of C18N2, C18B2, C18BN, and their molecular ions which originate from the C20 (Ih) cage were studied by the topological resonance energy (TRE) and the percentage topological resonance energy methods. The relationship between the aromaticity of C18BxNy isomers and the sites where the heteroatoms dope at the C20 (Ih) cage is discussed. Calculation results show that at the neutral and cationic states all the isomers are predicted to be antiaromatic with negative TREs, but their polyvalent anions are predicted to be aromatic with positive TREs. The most stable isomer is formed by heteroatom doping at the 1, 11-sites in C18N2, C18B2, and C18BN. Heterofullerenes are more aromatic than C20. The stability order in the neutral states is C18N2 > C18 BN > C18 B2 > C20. The stability order in closed-shell is C18B28- > C206- > C18BN6- > C18N24-. This predicts theoretically that their polyvalent anions have high aromaticity.

  1. Formulation of Organic Fertilizer Standard in Jiaxing City Based on NY525—2012

    OpenAIRE

    HUANG, Jinfa; NI, Xiongwei; Xie, Jianping; Shi, Yanping

    2013-01-01

    On the basis of research, analysis and argumentation, we conduct analysis and research on the current situation of quality of commercial organic fertilizer in Jiaxing City, and carry out the inspection and testing of organic matter indicators using the laboratory. Based on the standard of NY525-2012 Organic Fertilizer by the Ministry of Agriculture, this paper formulates the quality level indicators of local commercial organic fertilizer and adding specifications of raw materials.

  2. Evaluation of Photocatalytic Properties of Portland Cement Blended with Titanium Oxynitride (TiO2−xNy Nanoparticles

    Directory of Open Access Journals (Sweden)

    Juan D. Cohen

    2015-07-01

    Full Text Available Photocatalytic activity of Portland cement pastes blended with nanoparticles of titanium oxynitride (TiO2−xNy was studied. Samples with different percentages of TiO2−xNy (0.0%, 0.5%, 1%, 3% and TiO2 (1%, 3% were evaluated in order to study their self-cleaning properties. The presence of nitrogen in the tetragonal structure of TiO2 was evidenced by X-ray diffraction (XRD as a shift of the peaks in the 2θ axis. The samples were prepared with a water/cement ratio of 0.5 and a concentration of Rhodamine B of 0.5 g/L. After 65 h of curing time, the samples were irradiated with UV lamps to evaluate the reduction of the pigment. The color analysis was carried out using a Spectrometer UV/Vis measuring the coordinates CIE (Commission Internationale de l’Eclairage L*, a*, b*, and with special attention to the reddish tones (Rhodamine B color which correspond to a* values greater than zero. Additionally, samples with 0.5%, 1%, 3% of TiO2−xNy and 1%, 3% of TiO2 were evaluated under visible light with the purpose of determining the Rhodamine B abatement to wavelengths greater than 400 nm. The results have shown a similar behavior for both additions under UV light irradiation, with 3% being the addition with the highest photocatalytic efficiency obtained. However, TiO2−xNy showed activity under irradiation with visible light, unlike TiO2, which can only be activated under UV light.

  3. Expression of MAGE-A3, NY-ESO-1, LAGE-1 and PRAME in urothelial carcinoma

    Science.gov (United States)

    Dyrskjøt, L; Zieger, K; Kissow Lildal, T; Reinert, T; Gruselle, O; Coche, T; Borre, M; Ørntoft, T F

    2012-01-01

    Background: The potential for cancer-testis (CT) antigens as targets for immunotherapy in cancer patients has been heavily investigated, and currently cancer vaccine trials based on the CT antigens, MAGE-A3 and NY-ESO-1, are being carried out. Methods: We used specific q-RT-PCR assays to analyse the expression of the CT genes MAGE-A3, NY-ESO-1 (CTAG1B), LAGE-1 (CTAG2) and PRAME in a panel of bladder tumours from 350 patients with long-term follow-up and detailed treatment information. Results: Overall, 43% of the tumours expressed MAGE-A3, 35% expressed NY-ESO-1, 27% expressed LAGE-1 and 20% expressed PRAME. In all, 56% of the tumours expressed at least one of the CT genes analysed. Univariate Cox regression analysis of CT gene expression in non-muscle-invasive tumours showed that expression of MAGE-A3 (P=0.026), LAGE-1 (P=0.001) and NY-ESO-1 (P=0.040) was significantly associated with a shorter progression-free survival. In addition, we found that patients with tumours expressing PRAME responded poorly to chemotherapy (P=0.02, χ2-test). Conclusion: Cancer-testis genes are frequently expressed in bladder cancer and especially in tumours of high stage and grade. In addition, the CT gene expression may have both prognostic and predictive value. Development of specific immunotherapy against the CT antigens in bladder cancer may ultimately increase patient survival. PMID:22596240

  4. Ny løn: Den centrale vision der fik det svært ved det lokale forhandlingsbord

    DEFF Research Database (Denmark)

    Ravn, Ib; Møller, Anna Marie

    2001-01-01

    afdelingsleder og tillidsrepræsentant. Lokale ledere og tillidsrepræsentanter har derfor måttet optræde i en ny rolle som lønforhandlere. Det har medført et stort behov for viden om, hvordan man gennemfører en forhandling om løn på den enkelte arbejdsplads. Hvordan starter man og hvordan slutter man? Hvordan...

  5. Rapid generation of NY-ESO-1-specific CD4+ THELPER1 cells for adoptive T-cell therapy

    Science.gov (United States)

    Kayser, Simone; Boβ, Cristina; Feucht, Judith; Witte, Kai-Erik; Scheu, Alexander; Bülow, Hans-Jörg; Joachim, Stefanie; Stevanović, Stefan; Schumm, Michael; Rittig, Susanne M; Lang, Peter; Röcken, Martin; Handgretinger, Rupert; Feuchtinger, Tobias

    2015-01-01

    Tumor-associated antigens such as NY-ESO-1 are expressed in a variety of solid tumors but absent in mature healthy tissues with the exception of germline cells. The immune system anti-cancer attack is mediated by cell lysis or induction of growth arrest through paralysis of tumor cells, the latter of which can be achieved by tumor-specific CD4+, IFNγ-producing THelper type 1 (TH1) cells. Translation of these immune-mediated mechanisms into clinical application has been limited by availability of immune effectors, as well as the need for complex in vitro protocols and regulatory hurdles. Here, we report a procedure to generate cancer-testis antigen NY-ESO-1-targeting CD4+ TH1 cells in vitro for cancer immunotherapy in the clinic. After in vitro sensitization by stimulating T cells with protein-spanning, overlapping peptide pools of NY-ESO-1 in combination with IL-7 and low dose IL-2, antigen-specific T cells were isolated using IFNγ capture technique and subsequently expanded with IL-2, IL-7 and IL-15. Large numbers of NY-ESO-1-specific CD4+ T cells with a TH1 cytokine profile and lower numbers of cytokine-secreting CD8+ T cells could be generated from healthy donors with a high specificity and expansion potential. Manufactured CD4+ T cells showed strong specific TH1-responses with IFNγ+, TNFα+, IL-2+ and induced cell cycle arrest and apoptosis in tumor cells. The protocol is GMP-grade and approved by the regulatory authorities. The tumor-antigen specific CD4+ TH1 lymphocytes can be adoptively transferred as a T-cell therapy to boost anticancer immunity and this novel cancer treatment approach is applicable to both T cells from healthy allogeneic donors as well as to autologous T cells derived from cancer patients. PMID:26155389

  6. Rapid generation of NY-ESO-1-specific CD4(+) THELPER1 cells for adoptive T-cell therapy.

    Science.gov (United States)

    Kayser, Simone; Boβ, Cristina; Feucht, Judith; Witte, Kai-Erik; Scheu, Alexander; Bülow, Hans-Jörg; Joachim, Stefanie; Stevanović, Stefan; Schumm, Michael; Rittig, Susanne M; Lang, Peter; Röcken, Martin; Handgretinger, Rupert; Feuchtinger, Tobias

    2015-05-01

    Tumor-associated antigens such as NY-ESO-1 are expressed in a variety of solid tumors but absent in mature healthy tissues with the exception of germline cells. The immune system anti-cancer attack is mediated by cell lysis or induction of growth arrest through paralysis of tumor cells, the latter of which can be achieved by tumor-specific CD4(+), IFNγ-producing THelper type 1 (TH1) cells. Translation of these immune-mediated mechanisms into clinical application has been limited by availability of immune effectors, as well as the need for complex in vitro protocols and regulatory hurdles. Here, we report a procedure to generate cancer-testis antigen NY-ESO-1-targeting CD4(+) TH1 cells in vitro for cancer immunotherapy in the clinic. After in vitro sensitization by stimulating T cells with protein-spanning, overlapping peptide pools of NY-ESO-1 in combination with IL-7 and low dose IL-2, antigen-specific T cells were isolated using IFNγ capture technique and subsequently expanded with IL-2, IL-7 and IL-15. Large numbers of NY-ESO-1-specific CD4(+) T cells with a TH1 cytokine profile and lower numbers of cytokine-secreting CD8(+) T cells could be generated from healthy donors with a high specificity and expansion potential. Manufactured CD4(+) T cells showed strong specific TH1-responses with IFNγ(+), TNFα(+), IL-2(+) and induced cell cycle arrest and apoptosis in tumor cells. The protocol is GMP-grade and approved by the regulatory authorities. The tumor-antigen specific CD4(+) TH1 lymphocytes can be adoptively transferred as a T-cell therapy to boost anticancer immunity and this novel cancer treatment approach is applicable to both T cells from healthy allogeneic donors as well as to autologous T cells derived from cancer patients.

  7. The New York City Urban Search and Rescue Team (NY-TF1): A Case Study of Interagency Effectiveness

    Science.gov (United States)

    2011-03-01

    can be incorporated into both the FDNY and NYPD responses to everyday multi-agency operations. Applying the appreciative inquiry (AI) method into...this thesis utilized appreciative inquiry in a single case study of the NY-TF1 model supported by a set of seven interviews with key task force...collaborative elements developed through an appreciative inquiry of the task force will provide a vision for future interagency operations between NYC’s

  8. P10_Nov2012_Oct2014: Fire Island, NY pre- and post- storm cross-shore profiles from November 2012 to October 2014.

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This spreadsheet consists of Fire Island, NY pre- and post- storm cross-shore profiles collected from November 2012 to October 2014. This dataset contains a set of...

  9. P10_Nov2012_Oct2014: Fire Island, NY pre- and post- storm cross-shore profiles from November 2012 to October 2014.

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This spreadsheet consists of Fire Island, NY pre- and post- storm cross-shore profiles collected from November 2012 to October 2014. This dataset contains a set of...

  10. Archive of Boomer and Sparker Data Collected During USGS Cruise DIAN 97032 Long Island, NY Inner Shelf -- Fire Island, 24 September - 19 October, 1997

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS Diane G 97032 cruise. The coverage is the nearshore of Long Island, NY...

  11. Archive of Boomer and Sparker Data Collected During USGS Cruise DIAN 97032 Long Island, NY Inner Shelf -- Fire Island, 24 September - 19 October, 1997

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This CD-ROM contains digital high resolution seismic reflection data collected during the USGS Diane G 97032 cruise. The coverage is the nearshore of Long Island, NY...

  12. The Tumor Antigen NY-ESO-1 Mediates Direct Recognition of Melanoma Cells by CD4+ T Cells after Intercellular Antigen Transfer.

    Science.gov (United States)

    Fonteneau, Jean Francois; Brilot, Fabienne; Münz, Christian; Gannagé, Monique

    2016-01-01

    NY-ESO-1-specific CD4(+) T cells are of interest for immune therapy against tumors, because it has been shown that their transfer into a patient with melanoma resulted in tumor regression. Therefore, we investigated how NY-ESO-1 is processed onto MHC class II molecules for direct CD4(+) T cell recognition of melanoma cells. We could rule out proteasome and autophagy-dependent endogenous Ag processing for MHC class II presentation. In contrast, intercellular Ag transfer, followed by classical MHC class II Ag processing via endocytosis, sensitized neighboring melanoma cells for CD4(+) T cell recognition. However, macroautophagy targeting of NY-ESO-1 enhanced MHC class II presentation. Therefore, both elevated NY-ESO-1 release and macroautophagy targeting could improve melanoma cell recognition by CD4(+) T cells and should be explored during immunotherapy of melanoma. Copyright © 2015 by The American Association of Immunologists, Inc.

  13. Blockade of CTLA-4 promotes the development of effector CD8+ T lymphocytes and the therapeutic effect of vaccination with an attenuated protozoan expressing NY-ESO-1.

    Science.gov (United States)

    Dos Santos, Luara Isabela; Galvão-Filho, Bruno; de Faria, Paula Cristina; Junqueira, Caroline; Dutra, Miriam Santos; Teixeira, Santuza Maria Ribeiro; Rodrigues, Maurício Martins; Ritter, Gerd; Bannard, Oliver; Fearon, Douglas Thomas; Antonelli, Lis Ribeiro; Gazzinelli, Ricardo Tostes

    2015-03-01

    The development of cancer immunotherapy has long been a challenge. Here, we report that prophylactic vaccination with a highly attenuated Trypanosoma cruzi strain expressing NY-ESO-1 (CL-14-NY-ESO-1) induces both effector memory and effector CD8(+) T lymphocytes that efficiently prevent tumor development. However, the therapeutic effect of such a vaccine is limited. We also demonstrate that blockade of Cytotoxic T Lymphocyte Antigen 4 (CTLA-4) during vaccination enhances the frequency of NY-ESO-1-specific effector CD8(+) T cells producing IFN-γ and promotes lymphocyte migration to the tumor infiltrate. As a result, therapy with CL-14-NY-ESO-1 together with anti-CTLA-4 is highly effective in controlling the development of an established melanoma.

  14. Source of and potential bio-indicator for the heavy metal pollution in Ny-(A)lesund, Arctic

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Three kinds of tundra plant samples including Dicranum angnstum(a type of boreal bryophyte) , PuccineUia phryganodes (a type of fringy p/ant),Salix polaris (a type of vascular plant) and surface soil were samples in 200 at Ny-Alesund of the Arctic.The levels of eight heavy metal elements (Hg, Pb, Cd, Cu, Zn, Ni, Fe and Mn) and three metal-like dements (As, Se, Sr) in the plant and soil samples of the areas within previous coal mining activities are significantly higher than those of other areas.The relative accumulation of these elements in these tundra plant samples is consistent with the one in the soft samples, especially in the areas affected by previous coal-mining activities.Thus, the pollution is apparently from local coal mining activity.Dicranum angustum has the highest concentrations among those elements, and it can be a good bio-indicator for heavy metal pollution in Ny(A)lesund.Though Ny(A)lesund is less polluted by heavy metal than nearby Northern European human living areas, but much more than the tundras of the Alaska, Greenland and the Antarctic.

  15. a-SiCxNy:H thin films for applications in solar cells as passivation and antireflective coatings

    Science.gov (United States)

    Swatowska, Barbara; Kluska, Stanisława; Lewińska, Gabriela; Golańska, Julia; Stapiński, Tomasz

    2016-12-01

    Amorphous a-SiCxNy:H thin films may be an alternative to a-Si:N:H coatings which are commonly used in silicon solar cells. This material was obtained by PECVD (13.56 MHz) method. The reaction gases used: silane, methane, nitrogen and ammonia. The structure of the layers were investigated by scanning electron microscopy (SEM) and infrared spectroscopy (FTIR). IR absorption spectra of a-SiCxNy:H layers confirmed the presence of various hydrogen bonds - it is important for passivation of Si structural defects. The ellipsometric measurements were implemented to determine the thickness of layers d, refractive index n, extinction coefficient k and energy gap Eg. The values of the energy gap of a-SiCxNy:H layers are in the range from 1.89 to 4.34 eV. The correlation between energy gap of materials and refractive index was found. Generally the introduction of N and/or C into the amorphous silicon network rapidly increases the Eg values.

  16. Functional T cells targeting NY-ESO-1 or Melan-A are predictive for survival of patients with distant melanoma metastasis.

    Science.gov (United States)

    Weide, Benjamin; Zelba, Henning; Derhovanessian, Evelyna; Pflugfelder, Annette; Eigentler, Thomas K; Di Giacomo, Anna Maria; Maio, Michele; Aarntzen, Erik H J G; de Vries, I Jolanda M; Sucker, Antje; Schadendorf, Dirk; Büttner, Petra; Garbe, Claus; Pawelec, Graham

    2012-05-20

    To analyze the prognostic relevance of circulating T cells responding to NY-ESO-1, Melan-A, MAGE-3, and survivin in patients with melanoma with distant metastasis. We examined 84 patients with follow-up after analysis (cohort A), 18 long-term survivors with an extraordinarily favorable course of disease before analysis (> 24 months survival after first occurrence of distant metastases; cohort B), and 14 healthy controls. Circulating antigen-reactive T cells were characterized by intracellular cytokine staining after in vitro stimulation. In cohort A patients, the presence of T cells responding to peptides from NY-ESO-1, Melan-A, or MAGE-3 and the M category according to the American Joint Committee on Cancer classification were significantly associated with survival. T cells responding to NY-ESO-1 and Melan-A (hazard ratios, 0.29 and 0.18, respectively) remained independent prognostic factors in Cox regression analysis and were superior to the M category in predicting outcome. Median survival of patients possessing T cells responding to NY-ESO-1, Melan-A, or both was 21 months, compared with 6 months for all others. NY-ESO-1-responsive T cells were detected in 70% of cohort A patients surviving > 18 months and in 50% of cohort B patients. Melan-A responses were found in 42% and 47% of patients in cohorts A and B, respectively. In contrast, the proportion was only 22% for NY-ESO-1 and 23% for Melan-A in those who died within 6 months. The presence of circulating T cells responding to Melan-A or NY-ESO-1 had strong independent prognostic impact on survival in advanced melanoma. Our findings support the therapeutic relevance of Melan-A and NY-ESO-1 as targets for immunotherapy.

  17. Tumor-reactive CD8+ T-cell responses after vaccination with NY-ESO-1 peptide, CpG 7909 and Montanide ISA-51: association with survival.

    Science.gov (United States)

    Karbach, Julia; Gnjatic, Sacha; Bender, Armin; Neumann, Antje; Weidmann, Eckhart; Yuan, Jianda; Ferrara, Cathy A; Hoffmann, Eric; Old, Lloyd J; Altorki, Nasser K; Jäger, Elke

    2010-02-15

    Peptide-based vaccines have led to the induction of antigen-specific CD8(+) T-cell responses in patients with NY-ESO-1 positive cancers. However, vaccine-induced T-cell responses did not generally correlate with improved survival. Therefore, we tested whether a synthetic CpG 7909 ODN (deoxycytidyl-deoxyguanosin oligodeoxy-nucleotides) mixed with NY-ESO-1 peptide p157-165 and incomplete Freund's adjuvants (Montanide(R) ISA-51) led to enhanced NY-ESO-1 antigen-specific CD8(+) immune responses in patients with NY-ESO-1 or LAGE-1 expressing tumors. Of 14 HLA-A2+ patients enrolled in the study, 5 patients withdrew prematurely because of progressive disease and 9 patients completed 1 cycle of immunization. Nine of 14 patients developed measurable and sustained antigen-specific CD8(+) T-cell responses: Four had detectable CD8+ T-cells against NY-ESO-1 after only 2 vaccinations, whereas 5 patients showed a late-onset but durable induction of NY-ESO-1 p157-165 specific T-cell response during continued vaccination after 4 months. In 6 patients, vaccine-induced antigen-specific T-cells became detectable ex vivo and reached frequencies of up to 0.16 % of all circulating CD8(+) T-cells. Postvaccine T-cell clones were shown to recognize and lyse NY-ESO-1 expressing tumor cell lines in vitro. In 6 of 9 patients developing NY-ESO-1-specific immune responses, a favorable clinical outcome with overall survival times of 43+, 42+, 42+, 39+, 36+ and 27+ months, respectively, was observed.

  18. Tumor-infiltrating NY-ESO-1-specific CD8+ T cells are negatively regulated by LAG-3 and PD-1 in human ovarian cancer.

    Science.gov (United States)

    Matsuzaki, Junko; Gnjatic, Sacha; Mhawech-Fauceglia, Paulette; Beck, Amy; Miller, Austin; Tsuji, Takemasa; Eppolito, Cheryl; Qian, Feng; Lele, Shashikant; Shrikant, Protul; Old, Lloyd J; Odunsi, Kunle

    2010-04-27

    NY-ESO-1 is a "cancer-testis" antigen frequently expressed in epithelial ovarian cancer (EOC) and is among the most immunogenic tumor antigens defined to date. In an effort to understand in vivo tolerance mechanisms, we assessed the phenotype and function of NY-ESO-1-specific CD8(+) T cells derived from peripheral blood lymphocytes (PBLs), tumor-infiltrating lymphocytes (TILs), and tumor-associated lymphocytes (TALs) of EOC patients with NY-ESO-1-expressing tumors, with or without humoral immunity to NY-ESO-1. Whereas NY-ESO-1-specific CD8(+) T cells were readily detectable ex vivo with tetramers in TILs and TALs of seropositive patients, they were only detectable in PBLs following in vitro stimulation. Compared with PBLs, tumor-derived NY-ESO-1-specific CD8(+) T cells demonstrated impaired effector function, preferential usage of dominant T-cell receptor, and enriched coexpression of inhibitory molecules LAG-3 and PD-1. Expression of LAG-3 and PD-1 on CD8(+) T cells was up-regulated by IL-10, IL-6 (cytokines found in tumor ascites), and tumor-derived antigen-presenting cells. Functionally, CD8(+)LAG-3(+)PD-1(+) T cells were more impaired in IFN-gamma/TNF-alpha production compared with LAG-3(+)PD-1(-) or LAG-3(-)PD-1(-) subsets. Dual blockade of LAG-3 and PD-1 during T-cell priming efficiently augmented proliferation and cytokine production by NY-ESO-1-specific CD8(+) T cells, indicating that antitumor function of NY-ESO-1-specific CD8(+) T cells could potentially be improved by therapeutic targeting of these inhibitory receptors.

  19. Immunohistochemical expression of tumor antigens MAGE-A3/4 and NY-ESO-1 in renal oncocytoma and chromophobe renal cell carcinoma.

    Science.gov (United States)

    Demirović, Alma; Džombeta, Tihana; Tomas, Davor; Spajić, Borislav; Pavić, Ivana; Hudolin, Tvrtko; Milošević, Milan; Cupić, Hrvoje; Krušlin, Božo

    2010-10-15

    The distinction between renal oncocytoma (RO) and chromophobe renal cell carcinoma (ChRCC), especially the eosinophilic variant, can often be difficult. Our study has documented for the first time the expression of MAGE-A3/4 and NY-ESO-1 cancer testis antigens (CTAs) in these tumors. A total of 35 patients (17 ROs and 18 ChRCCs) were included in the study. Two antibodies were used for immunohistochemical staining: 57B recognizing multiple MAGE-A and D8.38 recognizing NY-ESO-1 CTAs. Fifteen (88.2%) samples of RO stained positively for both MAGE-A3/4 and NY-ESO-1 antigens. Regarding ChRCC, seven (38.9%) stained positively for MAGE-A3/4 and six (33.3%) for NY-ESO-1 antigens. Median MAGE-A3/4 expression was moderately positive in RO and negative in ChRCC. The difference in MAGE-A3/4 expression between two tumor groups was significant (P=0.0013). Median NY-ESO-1 expression was strongly positive in RO and negative in ChRCC. The difference in NY-ESO-1 expression between two tumor groups was also significant (P=0.0008). Our study has shown that RO had a significantly higher expression of both CTAs. However, additional research is needed to clarify their potential diagnostic implications.

  20. Centrosomal localisation of the cancer/testis (CT) antigens NY-ESO-1 and MAGE-C1 is regulated by proteasome activity in tumour cells.

    Science.gov (United States)

    Pagotto, Anna; Caballero, Otavia L; Volkmar, Norbert; Devalle, Sylvie; Simpson, Andrew J G; Lu, Xin; Christianson, John C

    2013-01-01

    The Cancer/Testis (CT) antigen family of genes are transcriptionally repressed in most human tissues but are atypically re-expressed in many malignant tumour types. Their restricted expression profile makes CT antigens ideal targets for cancer immunotherapy. As little is known about whether CT antigens may be regulated by post-translational processing, we investigated the mechanisms governing degradation of NY-ESO-1 and MAGE-C1 in selected cancer cell lines. Inhibitors of proteasome-mediated degradation induced the partitioning of NY-ESO-1 and MAGE-C1 into a detergent insoluble fraction. Moreover, this treatment also resulted in increased localisation of NY-ESO-1 and MAGE-C1 at the centrosome. Despite their interaction, relocation of either NY-ESO-1 or MAGE-C1 to the centrosome could occur independently of each other. Using a series of truncated fragments, the regions corresponding to NY-ESO-1(91-150) and MAGE-C1(900-1116) were established as important for controlling both stability and localisation of these CT antigens. Our findings demonstrate that the steady state levels of NY-ESO-1 and MAGE-C1 are regulated by proteasomal degradation and that both behave as aggregation-prone proteins upon accumulation. With proteasome inhibitors being increasingly used as front-line treatment in cancer, these data raise issues about CT antigen processing for antigenic presentation and therefore immunogenicity in cancer patients.

  1. Ground-based lidar measurements from Ny-Ålesund during ASTAR 2007

    Directory of Open Access Journals (Sweden)

    A. Herber

    2009-11-01

    Full Text Available During the Arctic Study of Tropospheric Aerosol, Clouds and Radiation (ASTAR in March and April 2007, measurements obtained at the AWIPEV Arctic Research Base in Ny-Ålesund, Spitsbergen at 78.9° N, 11.9° E (operated by the Alfred Wegener Institute for Polar and Marine Research – AWI and the Institut polaire français Paul-Emile Victor – IPEV, supported the airborne campaign. This included lidar data from the Koldewey Aerosol Raman Lidar (KARL and the Micro Pulse Lidar (MPL, located in the atmospheric observatory as well as photometer data and the daily launched radiosonde. The MPL features nearly continuous measurements; the KARL was switched on whenever weather conditions allowed observations (145 h in 61 days. From 1 March to 30 April, 71 meteorological balloon soundings were performed and compared with the concurrent MPL measurements; photometer measurements are available from 18 March. For the KARL data, a statistical overview of particle detection based on their optical properties backscatter ratio and volume depolarization can be given. The altitudes of the occurrence of the named features (subvisible and visible ice and water as well as mixed-phase clouds, aerosol layers as well as their dependence on different air mass origins are analyzed. Although the spring 2007 was characterized by rather clean conditions, diverse case studies of cloud and aerosol occurrence during March and April 2007 are presented in more detail, including temporal development and main optical properties as depolarization, backscatter and extinction coefficients. Links between air mass origins and optical properties can be presumed but need further evidence.

  2. Missing-Presumed Eroded: Holocene Sedimentation Patterns in Seneca Lake, NY

    Science.gov (United States)

    Loddengaard, K.; Curtin, T. M.

    2006-12-01

    The Finger Lakes of New York underwent a significant change in climate during the transition from the Hypsithermal to the Neoglacial at ~5 ka. To determine the effects of changes in climate on sedimentation patterns in the northern region of Seneca Lake, NY, we analyzed three cores that were collected along a shallow to deep water transect. Analyses of magnetic susceptibility, organic matter and carbonate content, fossil content, and grain size at a 1-cm interval were used to correlate cores, identify periods of erosion or non- deposition and assess the relative truncation of the Holocene sediment record. The stratigraphy and sedimentology of the cores indicate the lake contains a spatially and temporally discontinuous sediment record. Preliminary results indicate that the early to mid-Holocene sediment record was preferentially eroded in the deepwater cores, between 20 and 50 m water depth. At the deepwater sites, there is a gap in the sediment record that spans between ~12 and ~5 ka. Conversely, the late Holocene record is more complete in the deepwater cores than in the shallow water cores (12-20 m water depth). This data suggests a significant change on the controls and location of erosion and deposition in the lake during the Holocene. We hypothesize that the early to mid-Holocene sediment may have been eroded by strong internal seiches during stormy conditions that were initiated by cooler temperatures and a change in the position of the jet stream during the Hypsithermal-Neoglacial transition. Erosion occurred primarily in shallow water sites (<~30 m) during the Neoglacial, perhaps as a result of a change in the number and frequency of storms or strength of the internal seiche.

  3. Whole-lake nitrate addition for control of methylmercury in mercury-contaminated Onondaga Lake, NY.

    Science.gov (United States)

    Matthews, David A; Babcock, David B; Nolan, John G; Prestigiacomo, Anthony R; Effler, Steven W; Driscoll, Charles T; Todorova, Svetoslava G; Kuhr, Kenneth M

    2013-08-01

    Methylmercury (MeHg) strongly bioaccumulates in aquatic food webs resulting in exposure to humans and wildlife through consumption of fish. Production of MeHg is promoted by anaerobic conditions and the supply of inorganic Hg (Hg(2+)), sulfate (SO4(2-)), and labile organic carbon. The anaerobic sediments of stratified lakes are particularly active zones for methylation of Hg(2+) and can be an important source of MeHg to the water column during summer anoxia and fall turnover. Nitrate (NO3(-)) addition has recently been proposed as a novel approach for the control of MeHg accumulation in the hypolimnia of Hg-contaminated lakes. In 2011, a whole-lake NO3(-) addition pilot test was conducted in Hg-contaminated Onondaga Lake, NY with the objective of limiting release of MeHg from the pelagic sediments to the hypolimnion through maintenance of NO3(-)-N concentrations >1mgN/L. A liquid calcium-nitrate solution was added to the hypolimnion as a neutrally buoyant plume approximately three times per week during the summer stratification interval. Maximum hypolimnetic concentrations of MeHg and soluble reactive phosphorus (SRP) decreased 94% and 95% from 2009 levels, suggesting increased sorption to Fe and Mn oxyhydroxides in surficial sediments as the regulating mechanism. Increased MeHg concentrations in the upper waters during fall turnover, which had been a generally recurring pattern, did not occur in 2011, resulting in decreased exposure of aquatic organisms to MeHg. Over the 1992-2011 interval, the hypolimnetic NO3(-) supply explained 85% and 95% of the interannual variations in hypolimnetic accumulations of SRP and MeHg, respectively.

  4. 胃癌中肿瘤/睾丸抗原的表达研究及NY-ESO-1蛋白的自身抗体检测%Analysis of CT antigen expression and humoral immunogenicity of NY-ESO-1 protein in gastric carcinoma

    Institute of Scientific and Technical Information of China (English)

    吴晓江; 王殮; 季加孚

    2005-01-01

    目的:分析胃癌中11种CT抗原(cancer/testis antigen)基因的表达分布及NY-ESO-1蛋白引发的自身体液免疫应答,为胃癌的特异性肿瘤疫苗治疗提供依据.方法:利用RT-PCR方法,检测101例胃癌患者肿瘤标本和对应正常胃黏膜中11种CT抗原基因的表达.ELISA方法检测抗NY-ESO-1抗体.利用NY-ESO-1的单抗E978,应用免疫组化方法检测组织切片中NY-ESO-1抗原物质.结果:101例胃癌患者中,74.3%的患者至少表达1种检测的CT抗原基因.12例患者表达NY-ESO-1 mRNA,5例可以检测到NY-ESO-1抗原蛋白以及针对NY-ESO-1抗原的体液免疫反应.结论:胃癌中表达多种CT抗原基因,表达率较高的MAGE-3,SSX-4和NY-ESO-1/LAGE-1蛋白等可作为肿瘤疫苗的备选抗原,其中NY-ESO-1蛋白的免疫原性得到验证.CT抗原的多价肿瘤疫苗在胃癌治疗中具有一定的可行性.

  5. Clinical implications of expression of MAGE-1 and NY-ESO-1 mRNAs in gastrointestinal stromal tumors%肿瘤-睾丸抗原MAGE-1和NY-ESO-1 mRNA在胃肠间质瘤中的表达

    Institute of Scientific and Technical Information of China (English)

    赖斌; 朱培谦; 朱胜昌; 罗洪亮

    2010-01-01

    目的:探讨肿瘤-睾丸抗原(CTA)MAGE-1和NY-ESO-1作为胃肠间质瘤(GISTs)免疫治疗特异性靶点及MAGE-1和NY-ESO-1 mRNA作为辅助GISTs危险度分级指标的可能性.及其与GISTs生物学行为的关系.方法:采用逆转录-聚合酶链反应(RT-PCR)技术,检测30例GISTs中MAGE-1和NY-ESO-1mRNA的表达,并取正常胃肠道组织作为阴性对照组,同时分析MAGE-1和NY-ESO-1mRNA表达与病理特征的关系.结果:正常对照组中无阳性表达,30例GISTs中,18例至少表达一种CTA,MAGE-1和NY-ESO-1 mRNA在GISTs中的表达率分别为30%和47%.MAGE-1和NY-ESO-1 mRNA表达与患者年龄、性别和病理类型无关,而与肿瘤生长部位、肿瘤大小及危险度分级有关(P0.05).结论:MAGE-1和NY-ESO-1 mRNA在GISTs中的高特异性表达,其抗原有望成为GISTs免疫治疗特异性的靶点:MAGE-1和NY-ESO-1 mRNA表达与GISTs危险度分级有关,MAGE-1和NY-ESO-1 mRNA有望成为辅助GISTs危险度分级的诊断指标.

  6. pRV-Display-NY-ESO-1真核表达质粒的构建及其在Myc-CaP细胞株中的稳定表达%Construction of pRV-Display-NY-ESO-1 eukaryotic plasmid and its stable transfected Myc-CaP cell line

    Institute of Scientific and Technical Information of China (English)

    谢冲; 王国民

    2014-01-01

    Objective To construct the pRV-Display-NY-ESO-1 eukaryotic plasmid,and establish Myc-CaP cell line transfected with NY-ESO-1.Methods The full sequence of NY-ESO-1 was get from Genbank and amplified by polymerase chain reaction (PCR).Insert the fragments into p-Display eukaryotic expression vector.The recombinant plasmid with targeted gene was identified by digestion.Display-NY-ESO-1 was amplified by PCR and inserted into pRV retroviral vector.Retrovirus was produced by Phoenix cell line,and transfected into Myc-CaP cell line.Results The recombinant plasmid pRV-Display-NY-ESO-1 was successfully constructed and identified by PCR and DNA sequencing.Green fluorescence was observed on Phoenix cells and Myc-CaP cells encoding with GFP.The purity of Myc-CaP cells encoded with NY-ESO-1 was more than 80% according to flow cytometry.Conclusion Myc-CaP cell line was stably transfected with pRV-Display-NY-ESO-1,which is the base for further experiments.%目的 构建pRV-Display-NY-ESO-1真核表达质粒,建立并检测稳定表达纽约人食管鳞状细胞癌抗原(NY-ESO-1)的Myc-CaP细胞株.方法 从Genbank查找NY-ESO-1基因全长序列,聚合酶链反应(PCR)扩增目的片段,克隆至pDisplay载体,构建pDisplay-NY-ESO-1真核表达质粒,双酶切证实NY-ESO-1插入后,PCR扩增并定向连入pRV载体,构建pRV-Display-NY-ESO-1真核表达质粒,同时构建pDisplay-绿色荧光蛋白(GFP)作为阳性对照.经酶切、测序鉴定后,用Phoenix细胞产生逆转录病毒,脂质体转染法将重组质粒转入Myc-CaP细胞,并用Western blot和流式细胞术鉴定稳定转染的Myc-CaP细胞中NY-ESO-1的表达.结果 经PCR、测序鉴定成功构建pRV-Display-NY-ESO-1真核重组质粒.表达GFP的Phoenix细胞和Myc-CaP细胞在倒置荧光显微镜下均可见绿色荧光.Western blot检测Myc-CaP细胞中NY-ESO-1表达阳性,流式细胞术鉴定携带目的基因的Myc-CaP细胞纯度均大于80%.结论 pRV-Display-NY-ESO-1真核重组质粒稳定高表达于Myc-CaP细胞.

  7. Efficacy of vaccination with recombinant vaccinia and fowlpox vectors expressing NY-ESO-1 antigen in ovarian cancer and melanoma patients.

    Science.gov (United States)

    Odunsi, Kunle; Matsuzaki, Junko; Karbach, Julia; Neumann, Antje; Mhawech-Fauceglia, Paulette; Miller, Austin; Beck, Amy; Morrison, Carl D; Ritter, Gerd; Godoy, Heidi; Lele, Shashikant; duPont, Nefertiti; Edwards, Robert; Shrikant, Protul; Old, Lloyd J; Gnjatic, Sacha; Jäger, Elke

    2012-04-10

    Recombinant poxviruses (vaccinia and fowlpox) expressing tumor-associated antigens are currently being evaluated in clinical trials as cancer vaccines to induce tumor-specific immune responses that will improve clinical outcome. To test whether a diversified prime and boost regimen targeting NY-ESO-1 will result in clinical benefit, we conducted two parallel phase II clinical trials of recombinant vaccinia-NY-ESO-1 (rV-NY-ESO-1), followed by booster vaccinations with recombinant fowlpox-NY-ESO-1 (rF-NY-ESO-1) in 25 melanoma and 22 epithelial ovarian cancer (EOC) patients with advanced disease who were at high risk for recurrence/progression. Integrated NY-ESO-1-specific antibody and CD4(+) and CD8(+) T cells were induced in a high proportion of melanoma and EOC patients. In melanoma patients, objective response rate [complete and partial response (CR+PR)] was 14%, mixed response was 5%, and disease stabilization was 52%, amounting to a clinical benefit rate (CBR) of 72% in melanoma patients. The median PFS in the melanoma patients was 9 mo (range, 0-84 mo) and the median OS was 48 mo (range, 3-106 mo). In EOC patients, the median PFS was 21 mo (95% CI, 16-29 mo), and median OS was 48 mo (CI, not estimable). CD8(+) T cells derived from vaccinated patients were shown to lyse NY-ESO-1-expressing tumor targets. These data provide preliminary evidence of clinically meaningful benefit for diversified prime and boost recombinant pox-viral-based vaccines in melanoma and ovarian cancer and support further evaluation of this approach in these patient populations.

  8. MAGEA4 expression in bone and soft tissue tumors: its utility as a target for immunotherapy and diagnostic marker combined with NY-ESO-1.

    Science.gov (United States)

    Iura, Kunio; Kohashi, Kenichi; Ishii, Takeaki; Maekawa, Akira; Bekki, Hirofumi; Otsuka, Hiroshi; Yamada, Yuichi; Yamamoto, Hidetaka; Matsumoto, Yoshihiro; Iwamoto, Yukihide; Oda, Yoshinao

    2017-07-26

    Cancer-testis (CT) antigens have promise as targets for immunotherapy, because of their restricted expression in tumor or testis tissue. MAGEA4 is both a MAGE family member and a CT antigen, and has attracted attention as a potential immunotherapeutic target. We investigated MAGEA4 expression by immunohistochemistry in bone and soft tissue tumor specimens that consisted of 35 malignant or intermediate and 24 benign histological subtypes, in order to evaluate its possible utility as an immunotherapy target and its potential use as a diagnostic marker when combined with another CT antigen, NY-ESO-1. Among these tumors, MAGEA4 was detected in 82.2% of synovial sarcomas, 67.7% of myxoid liposarcomas, 43.8% of osteosarcomas, 41.4% of angiosarcomas, 24.6% of malignant peripheral nerve sheath tumors (MPNSTs), and 21.4% of chondrosarcomas. NY-ESO-1 expression was found in 88.2% of myxoid liposarcomas, 61.1% of synovial sarcomas, 31.3% of osteosarcomas, 21.4% of pleomorphic liposarcomas, 16.7% of desmoplastic small round cell tumors, and 14.3% of chondrosarcomas. Benign tumors and non-tumorous tissue, except for testis tissue, did not express MAGEA4 or NY-ESO-1. Combined use of MAGEA4 and NY-ESO-1 increased the sensitivity, specificity, positive predictive values, and negative predictive values for distinguishing synovial sarcoma from spindle cell tumors and other mimicking tumors, compared to individual use of MAGEA4 or NY-ESO-1. Our results support the immunotherapy targeting MAGEA4 or NY-ESO-1 can be an ancillary therapy in the above-mentioned tumors, and the potential utility of MAGEA4 as an ancillary diagnostic marker for synovial sarcoma combined with NY-ESO-1.

  9. A pilot trial using lymphocytes genetically engineered with an NY-ESO-1-reactive T-cell receptor: long-term follow-up and correlates with response.

    Science.gov (United States)

    Robbins, Paul F; Kassim, Sadik H; Tran, Thai L N; Crystal, Jessica S; Morgan, Richard A; Feldman, Steven A; Yang, James C; Dudley, Mark E; Wunderlich, John R; Sherry, Richard M; Kammula, Udai S; Hughes, Marybeth S; Restifo, Nicholas P; Raffeld, Mark; Lee, Chyi-Chia R; Li, Yong F; El-Gamil, Mona; Rosenberg, Steven A

    2015-03-01

    Although adoptive cell therapy can be highly effective for the treatment of patients with melanoma, the application of this approach to the treatment of other solid tumors has been limited. The observation that the cancer germline (CG) antigen NY-ESO-1 is expressed in 70% to 80% and in approximately 25% of patients with synovial cell sarcoma and melanoma, respectively, prompted us to perform this first-in-man clinical trial using the adoptive transfer of autologous peripheral blood mononuclear cells that were retrovirally transduced with an NY-ESO-1-reactive T-cell receptor (TCR) to heavily pretreated patients bearing these metastatic cancers. HLA-*0201 patients with metastatic synovial cell sarcoma or melanoma refractory to standard treatments and whose cancers expressed NY-ESO-1 received autologous TCR-transduced T cells following a lymphodepleting preparative chemotherapy. Response rates using Response Evaluation Criteria in Solid Tumors (RECIST), as well as immunologic correlates of response, are presented in this report. Eleven of 18 patients with NY-ESO-1(+) synovial cell sarcomas (61%) and 11 of 20 patients with NY-ESO-1(+) melanomas (55%) who received autologous T cells transduced with an NY-ESO-1-reactive TCR demonstrated objective clinical responses. The estimated overall 3- and 5-year survival rates for patients with synovial cell sarcoma were 38% and 14%, respectively, whereas the corresponding estimated survival rates for patients with melanoma were both 33%. The adoptive transfer of autologous T cells transduced with a retrovirus encoding a TCR against an HLA-A*0201 restricted NY-ESO-1 epitope can be an effective therapy for some patients bearing synovial cell sarcomas and melanomas that are refractory to other treatments. ©2014 American Association for Cancer Research.

  10. NY-ESO-1 autoantibody as a tumor-specific biomarker for esophageal cancer: screening in 1969 patients with various cancers.

    Science.gov (United States)

    Oshima, Yoko; Shimada, Hideaki; Yajima, Satoshi; Nanami, Tatsuki; Matsushita, Kazuyuki; Nomura, Fumio; Kainuma, Osamu; Takiguchi, Nobuhiro; Soda, Hiroaki; Ueda, Takeshi; Iizasa, Toshihiko; Yamamoto, Naoto; Yamamoto, Hiroshi; Nagata, Matsuo; Yokoi, Sana; Tagawa, Masatoshi; Ohtsuka, Seiko; Kuwajima, Akiko; Murakami, Akihiro; Kaneko, Hironori

    2016-01-01

    Although serum NY-ESO-1 antibodies (s-NY-ESO-1-Abs) have been reported in patients with esophageal carcinoma, this assay system has not been used to study a large series of patients with various other cancers. Serum samples of 1969 cancer patients [esophageal cancer (n = 172), lung cancer (n = 269), hepatocellular carcinoma (n = 91), prostate cancer (n = 358), gastric cancer (n = 313), colorectal cancer (n = 262), breast cancer (n = 365)] and 74 healthy individuals were analyzed using an originally developed enzyme-linked immunosorbent assay system for s-NY-ESO-1-Abs. The optical density cut-off value, determined as the mean plus three standard deviations for serum samples from the healthy controls, was fixed at 0.165. Conventional tumor markers were also evaluated in patients with esophageal carcinoma. The positive rate of s-NY-ESO-1-Abs in patients with esophageal cancer (31 %) was significantly higher than that in the other groups: patients with lung cancer (13 %), patients with hepatocellular carcinoma (11 %), patients with prostate cancer (10 %), patients with gastric cancer (10 %), patients with colorectal cancer (8 %), patients with breast cancer (7 %), and healthy controls (0 %). The positive rate of s-NY-ESO-1-Abs was comparable to that of serum p53 antibodies (33 %), squamous cell carcinoma antigen (36 %), carcinoembryonic antigen (26 %), and CYFRA 21-1 (18 %) and gradually increased with the tumor stage. The positive rate of s-NY-ESO-1-Abs was significantly higher in patients with esophageal cancer than in patients with the other types of cancers. On the basis of its high specificity and sensitivity, even in patients with stage I tumors, s-NY-ESO-1-Abs may be one of the first choices for esophageal cancer.

  11. Prevalence of plasma autoantibody against cancer testis antigen NY-ESO-1 in HTLV-1 infected individuals with different clinical status.

    Science.gov (United States)

    Shiohama, Yasuo; Naito, Tadasuke; Matsuzaki, Toshio; Tanaka, Reiko; Tomoyose, Takeaki; Takashima, Hiroshi; Fukushima, Takuya; Tanaka, Yuetsu; Saito, Mineki

    2017-07-17

    Detection of specific immune responses against cancer/testis antigen NY-ESO-1 was recently reported in patients with adult T-cell leukemia/lymphoma (ATL) and human T-cell leukemia virus type 1 (HTLV-1)-infected asymptomatic carriers (ACs). However, the relationship of the responses with the HTLV-1 proviral load (PVL) and the levels of viral gene expression remain unclear. We measured plasma levels of autoantibodies to NY-ESO-1 immunogenic tumor antigen in HTLV-1-infected individuals with different clinical status, and in healthy controls. Data were compared to tax and HBZ mRNA levels, and PVL. Plasma anti-NY-ESO-1 antibody was detectable in 13.7% (7/51) of ACs, 29.2% (38/130) of patients with HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP), and 18.9% (10/53) of patients with ATL. Anti-NY-ESO-1 plasma levels were significantly higher in patients with HAM/TSP than in patients with ATL or ACs. Anti-NY-ESO-1 levels were not associated with PVL or the expression levels of tax and HBZ mRNA among HTLV-1-infected individuals, regardless of clinical status. The present results indicate the strong humoral immune response against NY-ESO-1 in natural HTLV-1 infection, irrespective of the clinical status. The higher immunoreactivity against NY-ESO-1 is not simply associated with the levels of both HTLV-1 gene expression and the number of infected cells in vivo. Rather, it might reflect chronic and generalized immune activation in infected individuals.

  12. Arctic warming, moisture increase and circulation changes observed in the Ny-Ålesund homogenized radiosonde record

    Science.gov (United States)

    Maturilli, Marion; Kayser, Markus

    2017-04-01

    Compared to global warming, the feedback mechanisms of Arctic Amplification lead to an increase of surface temperature in the Arctic by a factor of two. Yet, the vertical structure of Arctic warming and its resulting radiative feedbacks are poorly understood. Here, we focus on the analysis of the atmospheric column above Ny-Ålesund (78.9° N, 11.9° E), Svalbard. At Ny-Ålesund, radiosondes have been launched on a daily basis since 1993 in support of synoptic observations. The obtained radiosonde measurements 1993 to 2014 have been homogenized accounting for instrumentation discontinuities and known errors in the manufacturer provided profiles. From the homogenized data record, a first upper-air climatology of wind, humidity and temperature above Ny-Ålesund is presented, forming the background for the analysis of changes detected during the 22-year period. Particularly during the winter season, a strong increase in atmospheric humidity and temperature is observed, with a significant warming of the free troposphere in January and February of up to 3 K per decade. This winter warming is even more pronounced in the boundary layer below 1 km, presumably amplified by local conditions including e.g. orographic effects or the boundary layer capping inversion. Also the largest contribution to the increasing atmospheric water vapour column originates from the lowermost 2 km of the atmosphere where specific humidity inversions are frequently observed. Yet, no increase in the water vapour contribution by humidity inversions is detected. Instead, we find an increase in the humidity content of the large scale background humidity profiles to be responsible for the observed increase in winter integrated water vapour. The observed difference in the frequency occurrence of wind directions in the free troposphere between the first and second half of the 22-year period implies that the large scale synoptic flow over Svalbard has changed over the years. During the winter season, the

  13. Antibody Responses to NY-ESO-1 in Primary Breast Cancer Identify a Subtype Target for Immunotherapy

    OpenAIRE

    2011-01-01

    The highly immunogenic human tumor antigen NY-ESO-1 (ESO) is a target of choice for anti-cancer immune therapy. In this study, we assessed spontaneous antibody (Ab) responses to ESO in a large cohort of patients with primary breast cancer (BC) and addressed the correlation between the presence of anti-ESO Ab, the expression of ESO in the tumors and their characteristics. We found detectable Ab responses to ESO in 1% of the patients. Tumors from patients with circulating Ab to ESO exhibited co...

  14. A Genetic Vaccine Encoding Chimeric NY-ESO-1 Induces Anticancer Immunity in A Mouse Model%嵌合NY-ESO-1 DNA疫苗诱导黑色素瘤小鼠模型抗肿瘤免疫的研究

    Institute of Scientific and Technical Information of China (English)

    谷银芳; 顾汉民; 周曙明; 吴炯; 蒋泽华

    2015-01-01

    NY-ESO-1作为一种肿瘤抗原,具有较强的免疫抗原性,并且已成为肿瘤候选疫苗之一.但由于目前大多应用NY-ESO-1多肽以及蛋白质疫苗,其临床试验效果欠佳,亟需更为有效的NY-ESO-1抗原设计的肿瘤免疫治疗出现.该研究的目的是探索将NY-ESO-1与具有增强免疫效应的五种因子分别重组成嵌合蛋白抗原,使其更有效地被加工、转运和呈递,以期找到最佳的基因佐剂,增强NY-ESO-1作为肿瘤治疗性DNA疫苗的免疫效果.采用电脉冲体内细胞高效转入的方法对C57BL/6小鼠进行-DNA免疫,发现用编码NY-ESO-1或连接有HSP70的嵌合体质粒免疫可诱导强烈的NY-ESO-1特异性IgG1反应.NY-ESO-1连接泛素的质粒免疫小鼠主要诱导NY-ESO-1特异性IgG2a反应,表明此基因佐剂诱导强的Thl免疫反应.与其他嵌合NY-ESO-1质粒免疫相比,Ny-ESO-1连接泛素的质粒免疫小鼠,有效地保护小鼠对有NY-ESO-1表达的B16F10黑色素瘤细胞系的挑战作用,证明强的Thl免疫反应对预防和治疗肿瘤具有重要作用.去除调节性T细胞(regulatory T cells,Treg)可进一步增强泛素-NY-ESO-1嵌合DNA疫苗治疗黑色素瘤的作用.此外,Ub-NY-ESO-1质粒结合编码异源黑素瘤抗原GP100和TRP-2的质粒免疫可诱导对抗含有NY-ESO-1表达的B16F10黑色素瘤的协同抗肿瘤免疫疗效.该研究结果表明,编码泛素-NY-ESO-1嵌合抗原的质粒DNA疫苗或与编码其他相关黑色素瘤抗原的质粒的联合可能是有效的治疗黑色素瘤的疫苗.

  15. Expression and clinical significance of MAGE and NY-ESO-1 cancer-testis antigens in adenoid cystic carcinoma of the head and neck.

    Science.gov (United States)

    Veit, Johannes A; Heine, Daniela; Thierauf, Julia; Lennerz, Jochen; Shetty, Subasch; Schuler, Patrick J; Whiteside, Theresa; Beutner, Dirk; Meyer, Moritz; Grünewald, Inga; Ritter, Gerd; Gnjatic, Sacha; Sikora, Andrew G; Hoffmann, Thomas K; Laban, Simon

    2016-07-01

    Adenoid cystic carcinoma (ACC) of the head and neck is a rare but highly malignant tumor. Cancer-testis antigens (CTAs) represent an immunogenic family of cancer-specific proteins and thus represent an attractive target for immunotherapy. Eighty-four cases of ACC were identified, the CTAs pan-Melanoma antigen (pan-MAGE; M3H67) and New York esophageal squamous cell carcinoma (NY-ESO-1; E978) were detected immunohistochemically (IHC) and correlated with clinical data. Expression of NY-ESO-1 was found in 48 of 84 patients (57.1%) and of pan-MAGE in 28 of 84 patients (31.2%). Median overall survival (OS) in NY-ESO-1 positive versus negative patients was 130.8 and 282.0 months (p = .223), respectively. OS in pan-MAGE positive versus negative patients was 105.3 and 190.5 months, respectively (p = .096). Patients expressing both NY-ESO-1 and pan-MAGE simultaneously had significantly reduced OS with a median of 90.5 months compared with 282.0 months in negative patients (p = .047). A significant fraction of patients with ACC show expression of the CTAs NY-ESO-1 and/or pan-MAGE with promising immunotherapeutic implications. © 2016 Wiley Periodicals, Inc. Head Neck 38: 1008-1016, 2016. © 2016 Wiley Periodicals, Inc.

  16. 癌-睾丸抗原NY-ESO-1在食管鳞状细胞癌中的表达及其意义%Detection of cancer-testis antigen NY-ESO- 1 expression in esophageal carcinoma using tissue microarray technique

    Institute of Scientific and Technical Information of China (English)

    吴晁; 陈同钰; 孟凡青; 樊祥山

    2007-01-01

    目的 检测NY-ESO-1蛋白在食管鳞癌中的表达,分析其与肿瘤临床病理参数的关系以及NY-ESO-1蛋白在晚期食管鳞癌免疫治疗中的意义.方法 选取113例中晚期食管鳞癌标本构建组织芯片,应用免疫组化EnVision二步法,检测NY-ESO-1蛋白的表达,SPSS统计软件分析所得数据.结果 109例有效标本中,有32例NY-ESO-1阳性(29.4%),阳性部位在细胞浆,10例正常食管黏膜中无-例表达NY-ESO-1蛋白.NY-ESO-1蛋白的表达主要集中在肿瘤细胞分化较好的Ⅰ级和Ⅱ级食管鳞癌,阳性率有显著性差异(P<0.01),与肿瘤的大小,浸润深度及有无淋巴结转移无关(P>0.05).结论 NY-ESO-1是目前已知的免疫原性最强肿瘤抗原,可诱导自发的体液免疫和细胞免疫反应,其在晚期食管鳞癌中的表达提示NY-ESO-1可作为食管癌的免疫治疗的靶点.

  17. Reliability, Availability, and Maintainability of the Heat Recovery Incinerator at Naval Station Mayport.

    Science.gov (United States)

    1984-10-01

    of Policy & Mgt. Energy, Div, Hartford. CT CORNELL UNIVERSITY Ithaca NY (Serials Dept. Engr Lib.) DAMES & MOORE LIBRARY LOS ANGELES. CA DRURY COLLEGE...Engineering. Chesson) UNIVERSITY OF FLORIDA Dept Arch., Morgan. Gainesville. FL UNIVERSITY OF HAWAII ( Colin Ramage) Dept of Meteorology Honolulu HI

  18. Structural, optical and electrical properties of reactively sputtered CrxNy films: Nitrogen influence on the phase formation

    Directory of Open Access Journals (Sweden)

    Mirjana Novaković

    2017-03-01

    Full Text Available The properties of various CrxNy films grown by direct current (DC reactive sputtering process with different values of nitrogen partial pressures (0, 2×10-4, 3.5×10-4 and 5×10-4 mbar were studied. The structural analysis of the samples was performed by using X-ray diffraction and transmission electron microscopy (TEM, while an elemental analysis was realized by means of Rutherford backscattering spectrometry. By varying nitrogen partial pressure the pure Cr layer, mixture of Cr, Cr2N and CrN phases, or single-phase CrN was produced. TEM analysis showed that at pN2 = 2×10-4 mbar the layer has dense microstructure. On the other hand, the layer deposited at the highest nitrogen partial pressure exhibits pronounced columnar structure. The optical properties of CrxNy films were evaluated from spectroscopic ellipsometry data by the Drude or combined Drude and Tauc-Lorentz model. It was found that both refractive index and extinction coefficient are strongly dependent on the dominant phase formation (Cr, Cr2N, CrN during the deposition process. Finally, the electrical studies indicated the metallic character of Cr2N phase and semiconducting behaviour of CrN.

  19. The Banana Project. III. Spin-orbit Alignment in the Long-period Eclipsing Binary NY Cephei

    CERN Document Server

    Albrecht, Simon; Carter, Joshua; Snellen, Ignas; de Mooij, Ernst

    2010-01-01

    Binaries are not always neatly aligned. Previous observations of the DI Her system showed that the spin axes of both stars are highly inclined with respect to one another and the orbital axis. Here we report on a measurement of the spin-axis orientation of the primary star of the NY Cep system, which is similar to DI Her in many respects: it features two young early-type stars (~6 Myr, B0.5V+B2V), in an eccentric and relatively long-period orbit (e=0.48, P=15.d3). The sky projections of the rotation vector and the spin vector are well-aligned (beta_p = 2 +- 4 degrees), in strong contrast to DI Her. Although no convincing explanation has yet been given for the misalignment of DI Her, our results show that the phenomenon is not universal, and that a successful theory will need to account for the different outcome in the case of NY Cep.

  20. Modelling and Optimization for Deposition of SiOxNy Films by Radio-Frequency Reactive Sputtering

    Institute of Scientific and Technical Information of China (English)

    XU Wen-Bin; DONG Shu-Rong; WANG De-Miao

    2007-01-01

    SiOxNy films are deposited by reactive sputtering from a Si target in Ar/O2/N2 atmospheres. In order to achieve the control of film composition and to keep a high deposition rate at the same time, a new sputtering model based on Berg's work is provided for the condition of double reactive gases. Analysis based on this model shows that the deposition process can easily enter the target-poisoning mode when the preset gas flow (N2 in this work)is too high, and the film composition will change from nitrogen-rich to SiO2-like with the increase of oxygen supply while keeping the N2 supply constant. The modelling results are confirmed in the deposition process of SiOxNy. Target self-bias voltages during sputtering are measured to characterize the different sputtering modes.FTIR-spectra and dielectric measurements are used to testify the model prediction of composition. Finally, an optimized sputtering condition is selected with the O2/N2 flow ratio varying from 0 to 1 and N2 supply fixed at 1 sccm. Average deposition rate of 17nm/min is obtained under this selected condition, which has suggested the model validity and potential for industry applications.

  1. Immunization of HLA-A*0201 and/or HLA-DPβ1*04 Patients with Metastatic Melanoma Using Epitopes from the NY-ESO-1 Antigen

    Science.gov (United States)

    Khong, Hung T.; Yang, James C.; Topalian, Suzanne L.; Sherry, Richard M.; Mavroukakis, Sharon A.; White, Donald E.; Rosenberg, Steven A.

    2008-01-01

    HLA class I-restricted peptides are often used in peptide vaccine regimens. There is strong evidence that many of these peptides can generate specific CD8+ T-cell responses in vivo; however, only occasional objective clinical responses have been reported. To test whether provision of “help” would enhance antitumor immunity, the authors initiated a clinical trial in which patients with metastatic melanoma were immunized against the NY-ESO-1 tumor antigen, using an HLA-A2-restricted peptide (ESO-1:165V), an HLA-DP4-restricted peptide (NY-ESO-1:161-180), or both peptides given concomitantly. The first cohorts received only ESO-1:165V, using three vaccination schedules. Immunologically, most patients developed immune responses to the HLA-A2-restricted native ESO-1 epitope after vaccination. Peptide vaccine given daily for 4 days appeared to induce immunologic responses more rapidly than if given once a week or once every 3 weeks. In contrast, vaccination using the NY-ESO-1:161-180 peptide induced immune responses in only a few patients. Clinically, one patient who received NY-ESO-1:161-180 peptide alone had a partial response lasing 12 months. Concomitant vaccination with the HLA class II-restricted peptide did not alter the immune response to the HLA class I-restricted peptide form NY-ESO-1. However, vaccination with the HLA-A2-restricted epitope generated primarily T cells that did not recognize tumor after in vitro sensitization. This result raises questions about the use of synthetic peptides derived from NY-ESO-1 as a sole form of immunization. PMID:15534491

  2. Real-time Monitoring Network to Characterize Anthropogenic and Natural Events Affecting the Hudson River, NY

    Science.gov (United States)

    Islam, M. S.; Bonner, J. S.; Fuller, C.; Kirkey, W.; Ojo, T.

    2011-12-01

    The Hudson River watershed spans 34,700 km2 predominantly in New York State, including agricultural, wilderness, and urban areas. The Hudson River supports many activities including shipping, supplies water for municipal, commercial, and agricultural uses, and is an important recreational resource. As the population increases within this watershed, so does the anthropogenic impact on this natural system. To address the impacts of anthropogenic and natural activities on this ecosystem, the River and Estuary Observatory Network (REON) is being developed through a joint venture between the Beacon Institute, Clarkson University, General Electric Inc. and IBM Inc. to monitor New York's Hudson and Mohawk Rivers in real-time. REON uses four sensor platform types with multiple nodes within the network to capture environmentally relevant episodic events. Sensor platform types include: 1) fixed robotic vertical profiler (FRVP); 2) mobile robotic undulating platform (MRUP); 3) fixed acoustic Doppler current profiler (FADCP) and 4) Autonomous Underwater Vehicle (AUV). The FRVP periodically generates a vertical profile with respect to water temperature, salinity, dissolved oxygen, particle concentration and size distribution, and fluorescence. The MRUP utilizes an undulating tow-body tethered behind a research vessel to measure the same set of water parameters as the FRVP, but does so 'synchronically' over a highly-resolved spatial regime. The fixed ADCP provides continuous water current profiles. The AUV maps four-dimensional (time, latitude, longitude, depth) variation of water quality, water currents and bathymetry along a pre-determined transect route. REON data can be used to identify episodic events, both anthropogenic and natural, that impact the Hudson River. For example, a strong heat signature associated with cooling water discharge from the Indian Point nuclear power plant was detected with the MRUP. The FRVP monitoring platform at Beacon, NY, located in the

  3. Field Observations and Modeling of the Microphysics within Winter Storms over Long Island, NY

    Science.gov (United States)

    Stark, David

    Forecasting snowfall accumulation is challenging due to limitations and uncertainties in model bulk microphysical parameterizations (BMPs). The source of these errors is often unknown, since there have been relatively few in situ observations of the microphysics (ice habit, degree of riming, and snow density) during east coast winter storms. This thesis describes the microphysical evolution and model validation within east coast winter storms observed at Stony Brook, NY (SBNY) during the 2009-2010, 2010-2011, and 2011-2012 winter seasons. Surface microphysical measurements were taken every 15 to 30 minutes using a stereo microscope and camera, and snow depth and snow density were also recorded in 15 storms over SBNY. During these storms, a vertically-pointing Ku-band radar was used to observe the vertical evolution of reflectivity and Doppler vertical velocities. A Particle Size and Velocity (PARSIVEL) disdrometer was also used to measure the surface size distribution and fall speeds of snow at SBNY. Changes in the height of the maximum vertical motion relative to the favored growth temperatures led to changes in ice habit throughout the evolution in the comma head of extratropical cyclones and two heavy snow bands. Cold type ice habits with a few plates and dendrites were observed with light riming as the surface low was located along or east of the Mid-Atlantic coast. As the cyclone moved northward towards SBNY, moderately rimed dendrites, plates, and needles were observed. Heavily rimed needles and graupel were observed near the warm front and cyclone center. Mainly needles with light riming and a snow-liquid ratio from 8:1 to 9:1 were observed 2 to 4 hours before two heavy snow bands. With the strongest frontogenetical ascent during snow band maturity, moderately rimed dendrites were observed with snow-liquid ratios from 11:1 to 13:1. Lightly rimed plates and a snow-liquid ratio of 8:1 were observed after the heavy snow bands. The WSM6, MORR, THOM2, and SBU

  4. MAGE-A和NY-ESO-1的表达与子宫颈癌临床病理特征及预后关系探讨

    Institute of Scientific and Technical Information of China (English)

    杨碧辉; 梁利斌

    2013-01-01

    Objective To explore the relationship between the expression of MAGE-A,NY-ESO-1 and the clinical pathologic features and prognosis of cervical cancer and to observe the influence of neoadjuvant chemotherapy on the expression of MAGE-A and NY-ESO-1. Methods 82 cases were divided into two groups. Group A,34 patients of FIGO Ⅰa1-Ⅰb1, who received hysterectomy. Group B,48 patients of FIGO Ⅰb2-Ⅳa,who received neoadjuvant chemotherapy followed by hysterectomy. Immunohistochemistry was used to detect the expressions of MAGE-A,NY-ESO-1 in 82 cases of cervical cancer. Analysis the relationship between the expression of MAGE-A,NY-ESO-1 and pathological properties or prognosis. Results In group A,the positive expression reates of MAGE-A and NY-ESO-1 were 44.1% and 58.8%. In group B,the positive expression reates of MAGE-A and NY-ESO-1 were 56.3% and 54.2% before neoadjuvant chemotherapy. After neoadjuvant chemotherapy, the positive expression reates of MAGE-A and NY-ESO-1 were 53.5% and 55.8%. The positive expression reates of MAGE-A and NY-ESO-1 of tumor diameter ≥2cm were higher than <2cm in group A. The expression of MAGE-A was related with N stage. The survival time of patient with MAGE-A positive was lower than negative(P<0.05). Conclusion MAGE-A and NY-ESO-1 frequently express in cervical cancer. Drugs of chemotherapy were not influence the expression of MAGE-A and NY-ESO-1. MAGE-A is related with tumor diameter and lymph node metastases.%  目的探讨子宫颈癌肿瘤组织中MAGE-A、NY-ESO-1的表达及与临床病理特征、预后关系。方法82例子宫颈癌患者分为两组。A组34例,FIGOⅠa1-Ⅰb1,接受根治性子宫切除术;B组48例,FIGOⅠb2-Ⅳa,接受新辅助化疗方案后行根治性子宫切除术。免疫组化法检测82例患者肿瘤组织中MAGE-A、NY-ESO-1的表达,并与患者临床病理特征及生存时间分析比较。结果 A组MAGE-A、NY-ESO-1阳性率分别为44.1%(15/34)和58.8%(20/34)。B

  5. 肿瘤-睾丸抗原NY- ESO-1在舌鳞状细胞癌中的表达及意义%Expression and Significance of Cancer - Testis Antigen NY - ESO - 1 in Human Tongue Squamous Cell Carcinoma

    Institute of Scientific and Technical Information of China (English)

    张行炜; 邵益森; 李慧; 黄辉

    2011-01-01

    目的:检测NY- ESO-1蛋白在舌鳞癌(tongue squamous cell carcinoma,TSCC)中的表达情况,分析其与肿瘤临床病理参数的关系,并探讨其作为TSCC免疫治疗靶标的可能性.方法:采用免疫组织化学EnVision法检测53例TSCC标本及10例正常舌黏膜中NY- ESO-1蛋白的表达,SPSS统计软件分析所得数据.结果:53例TSCC标本中13例NY- ESO-1阳性表达(24.5%),定位于细胞浆,而10例正常舌黏膜无表达.NY- ESO-1蛋白的表达与肿瘤大小及临床病理分级有关(P<0.05),但与肿瘤浸润深度及有无淋巴结转移无关.结论:NY-ESO-1蛋白在中高分化TSCC组织中的表达较高,可以进一步研究其作为TSCC免疫治疗靶点的可行性.%Objective: To detect the expression of cancer-testis antigen NY-ESO-1 in human tongue squamous cell carcinoma(TSCC), and to analyze its expression patterns with clinicopathologic parameters and explore it as a possible therapeutic targets immune of TSCC. Methods: Immunohistochemical EnVision techniques was used to analyze the NY-ESO-1 expression in 53 cases of TSCC and 10 cases of normal tongue mucosa. Statistical analysis was done with SPSS. Results: Expression of NY-ESO-1 was observed in 13 cases of 53 samples(24. 5%) , but none in normal tongue mucosa. The expression of NY-ESO-1 was significantly associated with pathological grade and tumor size, but not with positive lymph node status and tumor infiltration depth. Conclusion: The higher expression of NY-ESO-1 in the well differentiated TSCC takes a possibility to explore it as a therapeutic targets immune of TSCC.

  6. Processing and cross-presentation of individual HLA-A, -B, or -C epitopes from NY-ESO-1 or an HLA-A epitope for Melan-A differ according to the mode of antigen delivery.

    Science.gov (United States)

    Robson, Neil C; McAlpine, Tristan; Knights, Ashley J; Schnurr, Max; Shin, Amanda; Chen, Weisan; Maraskovsky, Eugene; Cebon, Jonathan

    2010-07-15

    The ability of dendritic cells (DCs) to cross-present protein tumor antigens to cytotoxic T lymphocytes (CTLs) underpins the success of therapeutic cancer vaccines. We studied cross-presentation of the cancer/testis antigen, NY-ESO-1, and the melanoma differentiation antigen, Melan-A by human DC subsets. Monocyte-derived DCs (MoDCs) efficiently cross-presented human leukocyte associated (HLA)-A2-restricted epitopes from either a formulated NY-ESO-1/ISCOMATRIX vaccine or when either antigen was mixed with ISCOMATRIX adjuvant. HLA-A2 epitope generation required endosomal acidification and was proteasome-independent for NY-ESO-1 and proteasome-dependent for Melan-A. Both MoDCs and CD1c(+) blood DCs cross-presented NY-ESO-1-specific HLA-A2(157-165)-, HLA-B7(60-72)-, and HLA-Cw3(92-100)-restricted epitopes when formulated as an NY-ESO-1/ISCOMATRIX vaccine, but this was limited when NY-ESO-1 and ISCOMATRIX adjuvant were added separately to the DC cultures. Finally, cross-presentation of NY-ESO-1(157-165)/HLA-A2, NY-ESO-1(60-72)/HLA-B7, and NY-ESO-1(92-100)/HLA-Cw3 epitopes was proteasome-dependent when formulated as immune complexes (ICs) but only proteasome-dependent for NY-ESO-1(60-72)/HLA-B7-restricted cross-presentation facilitated by ISCOMATRIX adjuvant. We demonstrate, for the first time, proteasome-dependent and independent cross-presentation of HLA-A-, B-, and C-restricted epitopes within the same full-length tumor antigen by human DCs. Our findings identify important differences in the capacities of human DC subsets to cross-present clinically relevant, full-length tumor antigens and how vaccine formulation impacts CTL responses in vivo.

  7. Ny uddannelse til senioridrætskonsulent styrker samarbejdet mellem DGI og University College Lillebælt

    DEFF Research Database (Denmark)

    Bendsen, Aksel

    2008-01-01

    I samarbejde med Danske Gymnastik og Idrætsforeninger har University College Lillebælt udviklet en ny uddannelse til senioridrætskonsulent. Uddannelsen startede i Odense i efteråret 2008. At udvikle uddannelsen involverede flere parter. Det krævede et stort samarbejde at få alle disse forskellige...... menneskers ønsker og behov til at resultere i en uddannelse med et klart sigte og et kvalificeret indhold. Det er imidlertid et krav til de nye professionshøjskoler, at de kan løse den slags opgaver. I dag er den nye uddannelse et bevis på, at University College Lillebælt har disse kompetencer....

  8. Characteristics of the boundary layerat Ny-Ålesund in the Arctic during the ARTIST field experiment

    Directory of Open Access Journals (Sweden)

    T. Georgiadis

    2003-06-01

    Full Text Available A preliminary analysis of boundary layer data acquired during the Arctic Radiation and Turbulence Interaction Study Experiment (ARTIST at Ny-Ålesund (Spitzbergen in 1998 is presented. As expected, the wind fi eld and the thermal structure of the boundary layer are strongly infl uenced by the katabatic fl ow blowing along the Kongsfjorden. In particular, if the large scale circulation has a component along the same direction, the resulting low level wind reaches velocities comparable with geostrophic wind. Stable to neutral conditions occurred most of the time, sustaining inversion or spiky layers respectively, depending on the wind intensity. The behaviour of some micrometeorological parameters was examined in relation to the general fl ow pattern and the characteristics of this area. The highest values of surface sensible heat fl ux (with negative sign were observed during daytime, in presence of forced convection, due to the transfer of warmer upper layer air into the surface layer.

  9. Crustal structure using receiver function in the east part of A'nyêmaqên suture belt

    Institute of Scientific and Technical Information of China (English)

    DUAN Yong-hong; ZHANG Xian-kang; LIU Zhi; XU Zhao-fan; WANG Fu-yun; PAN Ji-shun; LIANG Guo-jing

    2007-01-01

    Twenty broadband seismographs were deployed along Hongyuan, Sichuan to Wuwei, Gansu. 81 teleseismic events were recorded in one year. We computed receiver functions from teleseismic waveform data and obtained S wave velocity structure beneath each station along the profile by using receiver function inversion method. The results revealed that the crustal structure is very complex and crustal average S wave velocity is to be on the low side. Low velocity structure generally exists in the depth range of 10~40 km in the crust between Aba arc fault and northern edge fault of Qinling earth's axis and it is a tectonic feature of complex geological process such as ancient A'nyêmaqên Tethys ocean from closing and side colliding to subducted plate exhumed or thrust rock slice lifted. The Moho is about 50 km depth along the profile and is slightly deeper in the south than in the north.

  10. Edgar Morin et Patrick Singaïny, Avant, pendant après le 11 janvier

    OpenAIRE

    Keslassy, Eric

    2015-01-01

    Les attentats du 7, 8 et 9 janvier 2015 ont plongé la France dans la sidération. Un état de choc qui n’est pas propice à l’analyse. Edgar Morin et Patrick Singaïny tentent néanmoins de produire une réflexion visant à comprendre ce qui s’est passé. Ils s’efforcent également de proposer des pistes pour que cela n’arrive plus (même si le livre ne formule pas réellement de pistes concrètes). Le plan de l’ouvrage est conforme à son titre. Son objectif est en effet de réfléchir – rapidement – à tro...

  11. LV305, a dendritic cell-targeting integration-deficient ZVex(TM)-based lentiviral vector encoding NY-ESO-1, induces potent anti-tumor immune response.

    Science.gov (United States)

    Albershardt, Tina Chang; Campbell, David James; Parsons, Andrea Jean; Slough, Megan Merrill; Ter Meulen, Jan; Berglund, Peter

    2016-01-01

    We have engineered an integration-deficient lentiviral vector, LV305, to deliver the tumor antigen NY-ESO-1 to human dendritic cells in vivo through pseudotyping with a modified Sindbis virus envelop protein. Mice immunized once with LV305 developed strong, dose-dependent, multifunctional, and cytotoxic NY-ESO-1-specific cluster of differentiation 8 (CD8) T cells within 14 days post-immunization and could be boosted with LV305 at least twice to recall peak-level CD8 T-cell responses. Immunization with LV305 protected mice against tumor growth in an NY-ESO-1-expressing CT26 lung metastasis model, with the protective effect abrogated upon depletion of CD8 T cells. Adoptive transfer of CD8 T cells, alone or together with CD4 T cells or natural killer cells, from LV305-immunized donor mice to tumor-bearing recipient mice conferred significant protection against metastatic tumor growth. Biodistribution of injected LV305 in mice was limited to the site of injection and the draining lymph node, and injected LV305 exhibited minimal excretion. Mice injected with LV305 developed little to no adverse effects, as evaluated by toxicology studies adherent to good laboratory practices. Taken together, these data support the development of LV305 as a clinical candidate for treatment against tumors expressing NY-ESO-1.

  12. Optimal design of light trapping in thin-film solar cells enhanced with graded SiNx and SiOxNy structure.

    Science.gov (United States)

    Zhao, Yongxiang; Chen, Fei; Shen, Qiang; Zhang, Lianmeng

    2012-05-07

    In this paper, a graded SiNx and SiOxNy structure is proposed as antireflection coatings deposited on top of amorphous silicon (α-Si) thin-film solar cell. The structural parameters are optimized by differential evolution in order to enhance the optical absorption of solar cells to the greatest degree. The optimal design result demonstrates that the nonlinear profile of dielectric constant is superior to the linear profile, and discrete multilayer graded antireflection coatings can outperform near continuously graded antireflection coatings. What's more, the electric field intensity distributions clearly demonstrate the proposed graded SiNx and SiOxNy structure can remarkably increase the magnitude of electric field of a-Si:H layer and hence, enhance the light trapping of a-Si:H thin-film solar cells in the whole visible and near-infrared spectrum. Finally, we have compared the optical absorption enhancements of proposed graded SiNx and SiOxNy structure with nanoparticles structure, and demonstrated that it can result in higher enhancements compared to the dielectric SiC and TiO2 nanoparticles. We have shown that the optimal graded SiNx and SiOxNy structure optimized by differential evolution can reach 33.31% enhancement which has exceeded the ideal limit of 32% of nanoparticles structure including plasmonic Ag nanoparticles, dielectric SiC and TiO2 nanoparticles.

  13. LV305, a dendritic cell-targeting integration-deficient ZVexTM-based lentiviral vector encoding NY-ESO-1, induces potent anti-tumor immune response

    Science.gov (United States)

    Albershardt, Tina Chang; Campbell, David James; Parsons, Andrea Jean; Slough, Megan Merrill; ter Meulen, Jan; Berglund, Peter

    2016-01-01

    We have engineered an integration-deficient lentiviral vector, LV305, to deliver the tumor antigen NY-ESO-1 to human dendritic cells in vivo through pseudotyping with a modified Sindbis virus envelop protein. Mice immunized once with LV305 developed strong, dose-dependent, multifunctional, and cytotoxic NY-ESO-1-specific cluster of differentiation 8 (CD8) T cells within 14 days post-immunization and could be boosted with LV305 at least twice to recall peak-level CD8 T-cell responses. Immunization with LV305 protected mice against tumor growth in an NY-ESO-1-expressing CT26 lung metastasis model, with the protective effect abrogated upon depletion of CD8 T cells. Adoptive transfer of CD8 T cells, alone or together with CD4 T cells or natural killer cells, from LV305-immunized donor mice to tumor-bearing recipient mice conferred significant protection against metastatic tumor growth. Biodistribution of injected LV305 in mice was limited to the site of injection and the draining lymph node, and injected LV305 exhibited minimal excretion. Mice injected with LV305 developed little to no adverse effects, as evaluated by toxicology studies adherent to good laboratory practices. Taken together, these data support the development of LV305 as a clinical candidate for treatment against tumors expressing NY-ESO-1. PMID:27626061

  14. Analysis of GAGE, NY-ESO-1 and SP17 cancer/testis antigen expression in early stage non-small cell lung carcinoma

    DEFF Research Database (Denmark)

    Gjerstorff, Morten F; Pøhl, Mette; Olsen, Karen E

    2013-01-01

    NSCLC has shown promising results. However, little is known about the expression of other cancer/testis antigens in NSCLC. In the present study the expression of cancer/testis antigens GAGE, NY-ESO-1 and SP17 was investigated in patients with completely resected, early stage, primary NSCLC....

  15. Implementering af ny standard for forebyggelse af delir i forhold til ældre svagelige patienter > 65 år med hoftefraktur

    DEFF Research Database (Denmark)

    Bagger, Bettan; Rehder, Bente; Pedersen, Niels

    2013-01-01

    at identificere best evidence i forhold til brug af screeningsredskaber. På baggrund af det systematiske review er der udviklet en ny evidensbaseret instruks. Der er planlagt og afholdt undervisningssessioner af afdelingens personale i forhold til dokumentation samt vigtigheden af nasal ilt og inddragelse af de...

  16. Evidence of the Adoption and Implementation of a Statewide Childhood Obesity Prevention Initiative in the New York State WIC Program: The "NY Fit WIC" Process Evaluation

    Science.gov (United States)

    Sekhobo, Jackson P.; Egglefield, Katherine; Edmunds, Lynn S.; Shackman, Gene

    2012-01-01

    Process evaluations are critical in determining whether outcome evaluations are warranted. This study assessed the extent to which a childhood obesity prevention initiative, "NY Fit WIC", was adopted and implemented by the New York State Supplemental Nutrition Program for Women, Infants and Children (WIC). Process data came from…

  17. 77 FR 13592 - AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, Eagle Creek Land...

    Science.gov (United States)

    2012-03-07

    ... Energy Regulatory Commission AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources... Power, LLC, Eagle Creek Water Resources, LLC, and Eagle Creek Land Resources, LLC (transferees) filed an...) 805-1469. Transferees: Mr. Bernard H. Cherry, Eagle Creek Hydro Power, LLC, Eagle Creek...

  18. 75 FR 27332 - AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC; Eagle Creek Water Resources, LLC; Eagle Creek Land...

    Science.gov (United States)

    2010-05-14

    ... Energy Regulatory Commission AER NY-Gen, LLC; Eagle Creek Hydro Power, LLC; Eagle Creek Water Resources... Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC, and Eagle Creek Land Resources, LLC.... For the transferee: Mr. Paul Ho, Eagle Creek Hydro Power, LLC, Eagle Creek Water Resources, LLC,...

  19. Amorphous sub-nanometre Tb-doped SiOxNy/SiO2 superlattices for optoelectronics

    Science.gov (United States)

    Ramírez, Joan Manel; Wojcik, Jacek; Berencén, Yonder; Ruiz-Caridad, Alícia; Estradé, Sònia; Peiró, Francesca; Mascher, Peter; Garrido, Blas

    2015-02-01

    Amorphous sub-nanometre Tb-doped SiOxNy/SiO2 superlattices were fabricated by means of alternating deposition of 0.7 nm thick Tb-doped SiOxNy layers and of 0.9 nm thick SiO2 barrier layers in an electron-cyclotron-resonance plasma enhanced chemical vapour deposition system with in situ Tb-doping capability. High resolution transmission electron microscopy images showed a well-preserved superlattice morphology after annealing at a high temperature of 1000 °C. In addition, transparent indium tin oxide (ITO) electrodes were deposited by electron beam evaporation using a shadow mask approach to allow for the optoelectronic characterization of superlattices. Tb3+ luminescent spectral features were obtained using three different excitation sources: UV laser excitation (photoluminescence (PL)), under a bias voltage (electroluminescence (EL)) and under a highly energetic electron beam (cathodoluminescence (CL)). All techniques displayed Tb3+ inner transitions belonging to 5D4 levels except for the CL spectrum, in which 5D3 transition levels were also observed. Two competing mechanisms were proposed to explain the spectral differences observed between PL (or EL) and CL excitation: the population rate of the 5D3 state and the non-radiative relaxation rate of the 5D3-5D4 transition due to a resonant OH-mode. Moreover, the large number of interfaces (trapping sites) that electrons have to get through was identified as the main reason for observing a bulk-limited charge transport mechanism governed by Poole-Frenkel conduction in the J-V characteristic. Finally, a linear EL-J dependence was measured, with independent spectral shape and an EL onset voltage as low as 6.7 V. These amorphous sub-nanometre superlattices are meant to provide low-cost solutions in different areas including sensing, photovoltaics or photonics.

  20. 前列腺癌患者血清中NY-ESO-1的表达及其与Gleason评分的相关性%Expression of NY-ESO-1 in Serum of Prostate Cancer Patients and Its Relationship with Gleason Score

    Institute of Scientific and Technical Information of China (English)

    谢冲; 黄其伟; 唐群业; 王国民

    2014-01-01

    目的 检测NY-ESO-1在前列腺癌患者血清中的表达情况,研究其与Gleason评分的相关性.方法 收集148例前列腺癌、124例良性前列腺增生(BPH)和175例健康人群血清,用ELISA法检测NY-ESO-1抗原的表达水平,确定正常值范围,计算NY-ESO-1抗原表达阳性率、特异性和敏感度.用Westernblot方法对阳性血清进行验证,并用统计学方法分析其与Gleason评分的相关性.结果 NY-ESO-1抗原在前列腺癌和BPH患者血清中的阳性表达率分别为48.6%和9.6%,两组数据之间差异有统计学意义(P=0.021).以BPH患者组为对照,NY-ESO-1检测前列腺癌的敏感度和特异性分别为82.3%和41.9%;其中,在PSA 4~ 10 ng/ml人群中NY-ESO-1检测前列腺癌的敏感度和特异性分别为44.0%和33.9%.Spearman等级相关分析NY-ESO-1与Gleason评分呈正相关(r=0.872).结论 NY-ESO-1抗原在我国前列腺癌患者血清中有较高表达,NY-ESO-1抗原的表达水平与Gleason评分之间呈正相关.

  1. Analysis of GAGE, NY-ESO-1 and SP17 cancer/testis antigen expression in early stage non-small cell lung carcinoma.

    Science.gov (United States)

    Gjerstorff, Morten F; Pøhl, Mette; Olsen, Karen E; Ditzel, Henrik J

    2013-10-08

    The unique expression pattern and immunogenic properties of cancer/testis antigens make them ideal targets for immunotherapy of cancer. The MAGE-A3 cancer/testis antigen is frequently expressed in non-small cell lung cancer (NSCLC) and vaccination with MAGE-A3 in patients with MAGE-A3-positive NSCLC has shown promising results. However, little is known about the expression of other cancer/testis antigens in NSCLC. In the present study the expression of cancer/testis antigens GAGE, NY-ESO-1 and SP17 was investigated in patients with completely resected, early stage, primary NSCLC. Tumor biopsies from normal lung tissue and from a large cohort (n = 169) of NSCLC patients were examined for GAGE, NY-ESO-1 and SP17 protein expression by immunohistochemical analysis. The expression of these antigens was further matched to clinical and pathological features using univariate cox regression analysis. GAGE and NY-ESO-1 cancer/testis antigens were not expressed in normal lung tissue, while SP17 was expressed in ciliated lung epithelia. The frequency of GAGE, NY-ESO-1 and SP17 expression in NSCLC tumors were 26.0% (44/169), 11.8% (20/169) and 4.7% (8/169), respectively, and 33.1% (56/169) of the tumors expressed at least one of these antigens. In general, the expression of GAGE, NY-ESO-1 and SP17 was not significantly associated with a specific histotype (adenocarcinoma vs. squamous cell carcinoma), but high-level GAGE expression (>50%) was more frequent in squamous cell carcinoma (p = 0.02). Furthermore, the frequency of GAGE expression was demonstrated to be significantly higher in stage II-IIIa than stage I NSCLC (17.0% vs. 35.8%; p = 0.02). Analysis of the relation between tumor expression of GAGE and NY-ESO-1 and survival endpoints revealed no significant associations. Our study demonstrates that GAGE, NY-ESO-1 and SP17 cancer/testis antigens are candidate targets for immunotherapy of NSCLC and further suggest that multi-antigen vaccines may be beneficial.

  2. NY-ESO-1 is a sensitive and specific immunohistochemical marker for myxoid and round cell liposarcomas among related mesenchymal myxoid neoplasms.

    Science.gov (United States)

    Hemminger, Jessica A; Iwenofu, O Hans

    2013-09-01

    Myxoid and round cell liposarcomas constitute approximately one-third of all liposarcomas, a relatively common group of fat-derived soft tissue sarcomas. The histomorphology is a continuum between highly differentiated myxoid and poorly differentiated round cell components. The gold standard of diagnosis is dependent on histomorphology and/or identification of t(12;16)(q13;p11) translocation by cytogenetics or demonstration of DDIT3 rearrangements by fluorescence in situ hybridization. There are currently no diagnostic immunohistochemical stains available. The broad range of myxoid neoplasms in the differential diagnosis includes a variety of sarcomas. Given the notable differences in disease biology among myxoid neoplasms, which range from benign to aggressive, an accurate diagnosis is imperative for proper treatment and prognostication. Prompted by our recent study showing frequent expression of the cancer testis antigen NY-ESO-1 in myxoid and round cell liposarcomas, we sought to evaluate the utility of NY-ESO-1 as an immunohistochemical marker for myxoid and round cell liposarcoma among mesenchymal myxoid neoplasms within the differential diagnosis. Formalin-fixed, paraffin-embedded blocks were obtained for the following mesenchymal myxoid neoplasms (n=138): myxoid and round cell liposarcoma (n=38); well-differentiated liposarcoma (n=12); lipoma (n=20; 4 with myxoid change); extra-cardiac soft tissue myxoma (n=39); extraskeletal myxoid chondrosarcoma (n=12); myxofibrosarcoma (n=10: 5 low grade, 2 intermediate grade, 3 high grade); and low-grade fibromyxoid sarcoma (n=7). Utilizing standard immunohistochemistry protocols, full sections were stained with NY-ESO-1 (clone E978), and staining was assessed for intensity (1-2+), percentage of tumor positivity, and location. In all, 36/38 (95%) of the myxoid and round cell liposarcomas demonstrated NY-ESO-1 immunoreactivity. The majority of the positive cases (34/36; 94%) showed strong, homogenous staining (>50% tumor

  3. Expression of cancer/testis antigens in salivary gland carcinomas with reference to MAGE-A and NY-ESO-1 expression in adenoid cystic carcinoma.

    Science.gov (United States)

    Beppu, Shintaro; Ito, Yohei; Fujii, Kana; Saida, Kosuke; Takino, Hisashi; Masaki, Ayako; Murase, Takayuki; Kusafuka, Kimihide; Iida, Yoshiyuki; Onitsuka, Tetsuro; Yatabe, Yasushi; Hanai, Nobuhiro; Hasegawa, Yasuhisa; Ijichi, Kei; Murakami, Shingo; Inagaki, Hiroshi

    2017-08-01

    Cancer/testis antigens (CTAs) are detected in cancer cells but not in healthy normal tissues, with the exception of gametogenic tissues. CTAs are highly immunogenic proteins, and thus represent ideal targets for cytotoxic T-lymphocyte-mediated specific immune therapy. The aim of this study was to screen CTA expression in various types of salivary gland carcinoma and to clarify clinicopathological significance of MAGE-A and NY-ESO-1 expression in adenoid cystic carcinomas (AdCCs) of the salivary gland, which is one of the most common salivary gland carcinomas, and usually has a fatal outcome. We used immunohistochemistry to examine the expression of four CTAs (MAGE-A, NY-ESO-1, CT7, and GAGE7) in various types of salivary gland carcinoma (n = 95). When carcinoma cases were divided into low-grade and intermediate/high-grade types, NY-ESO-1 and CT7 were expressed more frequently in intermediate/high-grade carcinomas. We then focused on MAGE-A and NY-ESO-1 expression in a large cohort of adenoid cystic carcinomas (AdCCs) (n = 46). MAGE-A and NY-ESO-1 were frequently expressed in AdCC; specifically, MAGE-A was expressed in >60% of the AdCC cases. MAGE-A expression and tumour site (minor salivary gland) were identified as independent risk factors for locoregional tumour recurrence. These findings suggest that CTAs may be expressed in a variety of salivary gland carcinomas, especially in those with higher histological grades. In addition, MAGE-A, which is frequently expressed in AdCC cases, may be a useful prognostic factor for poorer locoregional recurrence-free survival. © 2017 John Wiley & Sons Ltd.

  4. Bi-specific TCR-anti CD3 redirected T-cell targeting of NY-ESO-1- and LAGE-1-positive tumors.

    Science.gov (United States)

    McCormack, Emmet; Adams, Katherine J; Hassan, Namir J; Kotian, Akhil; Lissin, Nikolai M; Sami, Malkit; Mujić, Maja; Osdal, Tereza; Gjertsen, Bjørn Tore; Baker, Deborah; Powlesland, Alex S; Aleksic, Milos; Vuidepot, Annelise; Morteau, Olivier; Sutton, Deborah H; June, Carl H; Kalos, Michael; Ashfield, Rebecca; Jakobsen, Bent K

    2013-04-01

    NY-ESO-1 and LAGE-1 are cancer testis antigens with an ideal profile for tumor immunotherapy, combining up-regulation in many cancer types with highly restricted expression in normal tissues and sharing a common HLA-A*0201 epitope, 157-165. Here, we present data to describe the specificity and anti-tumor activity of a bifunctional ImmTAC, comprising a soluble, high-affinity T-cell receptor (TCR) specific for NY-ESO-1157-165 fused to an anti-CD3 scFv. This reagent, ImmTAC-NYE, is shown to kill HLA-A2, antigen-positive tumor cell lines, and freshly isolated HLA-A2- and LAGE-1-positive NSCLC cells. Employing time-domain optical imaging, we demonstrate in vivo targeting of fluorescently labelled high-affinity NYESO-specific TCRs to HLA-A2-, NY-ESO-1157-165-positive tumors in xenografted mice. In vivo ImmTAC-NYE efficacy was tested in a tumor model in which human lymphocytes were stably co-engrafted into NSG mice harboring tumor xenografts; efficacy was observed in both tumor prevention and established tumor models using a GFP fluorescence readout. Quantitative RT-PCR was used to analyze the expression of both NY-ESO-1 and LAGE-1 antigens in 15 normal tissues, 5 cancer cell lines, 10 NSCLC, and 10 ovarian cancer samples. Overall, LAGE-1 RNA was expressed at a greater frequency and at higher levels than NY-ESO-1 in the tumor samples. These data support the clinical utility of ImmTAC-NYE as an immunotherapeutic agent for a variety of cancers.

  5. Determination and characterization of nitrous oxide reductase in Mourinobacter hydrocarbonoclasticus NY4%M.hydrocarbonoclasticus NY4中N2 OR的酶活测定及环境因素对酶活力的影响

    Institute of Scientific and Technical Information of China (English)

    王鹏胤; 高远; 高浩峰; 胡南

    2016-01-01

    氧化亚氮( N2 O)是一种主要的温室气体,其生物学减量控制已经成为研究热点。在自然环境中,氧化亚氮还原酶( N2 OR)是唯一能将N2 O还原成N2的酶。以1株能在高盐条件下进行厌氧及好氧反硝化作用的海杆菌Marinobacter hydrocarbonoclasticus NY4为研究对象,建立了一种稳定的N2 OR酶活检测方法,考察了pH、盐质量分数以及碳氮比( C/N)等环境因素对N2 OR酶活力的影响。结果发现:当pH为7时、盐质量分数为6%~8%、C/N为8时, N2 OR呈现出了最高的酶活。本实验结果丰富了对N2 OR酶学特征的了解,为N2 O生物减量控制提供了部分参考。%Nitrous oxide ( N2 O) is a major greenhouse gas,and its biological reduction has become a hot topic. Nitrous oxide reductase ( N2 OR ) is the only enzyme that can reduce N2 O to N2 in natural environments. Marinobacter hydrocarbonoclasticus NY4,a halophilic bacterium with the ability of anaerobic and aerobic denitrification,was used in this study�We established a stable method to determine the activity of N2 OR,and studied the influence of key environment factors on the activity of N2 OR including pH value, salt concentration and carbon/nitrogen ratio ( C/N) . When pH was at 7,the salinity was 6% and C/N was 8,strain NY⁃4 exhibited the highest N2 OR activity. The experimental data enriched the knowledge of the characteristics of N2 OR,and provided some references for the biological reduction of N2 O.

  6. Antibody responses to NY-ESO-1 in primary breast cancer identify a subtype target for immunotherapy.

    Science.gov (United States)

    Hamaï, Ahmed; Duperrier-Amouriaux, Karine; Pignon, Pascale; Raimbaud, Isabelle; Memeo, Lorenzo; Colarossi, Cristina; Canzonieri, Vincenzo; Perin, Tiziana; Classe, Jean-Marc; Campone, Mario; Jézéquel, Pascal; Campion, Loïc; Ayyoub, Maha; Valmori, Danila

    2011-01-01

    The highly immunogenic human tumor antigen NY-ESO-1 (ESO) is a target of choice for anti-cancer immune therapy. In this study, we assessed spontaneous antibody (Ab) responses to ESO in a large cohort of patients with primary breast cancer (BC) and addressed the correlation between the presence of anti-ESO Ab, the expression of ESO in the tumors and their characteristics. We found detectable Ab responses to ESO in 1% of the patients. Tumors from patients with circulating Ab to ESO exhibited common characteristics, being mainly hormone receptor (HR)⁻ invasive ductal carcinomas of high grade, including both HER2⁻ and HER2⁺ tumors. In line with these results, we detected ESO expression in 20% of primary HR⁻ BC, including both ESO Ab⁺ and Ab⁻ patients, but not in HR⁺ BC. Interestingly, whereas expression levels in ESO⁺ BC were not significantly different between ESO Ab⁺ and Ab⁻ patients, the former had, in average, significantly higher numbers of tumor-infiltrated lymph nodes, indicating that lymph node invasion may be required for the development of spontaneous anti-tumor immune responses. Thus, the presence of ESO Ab identifies a tumor subtype of HR⁻ (HER2⁻ or HER2⁺) primary BC with frequent ESO expression and, together with the assessment of antigen expression in the tumor, may be instrumental for the selection of patients for whom ESO-based immunotherapy may complement standard therapy.

  7. TiNy Titans: The Role of Dwarf-Dwarf Interactions in the Evolution of Low Mass Galaxies

    CERN Document Server

    Stierwalt, S; Patton, D; Johnson, K; Kallivayalil, N; Putman, M; Privon, G; Ross, G

    2014-01-01

    We introduce TiNy Titans (TNT), the first systematic study of star formation and the subsequent processing of the interstellar medium in interacting dwarf galaxies. Here we present the first results from a multiwavelength observational program based on a sample of 104 dwarf galaxy pairs selected from a range of environments within the SDSS and caught in various stages of interaction. The TNT dwarf pairs span mass ratios of M1/M2 100 A, occur in 20% of TNT dwarf pairs, regardless of environment, compared to only 6-8% of matched unpaired dwarfs. Starbursts can be triggered throughout the merger (out to large pair separations) and not just approaching coalescence. Despite their enhanced star formation, most TNT dwarf pairs have similar gas fractions relative to unpaired dwarfs. Thus, there may be significant reservoirs of diffuse, non-starforming gas surrounding the dwarf pairs or the gas consumption timescales may be long in the starburst phase. The only TNT dwarf pairs with low gas fractions (fgas <0.4) and...

  8. Water Residence Times and Their Relation to Soil and Aquifer Properties and Degree of Urbanization (Croton Water Supply Area, NY)

    Science.gov (United States)

    Vitvar, T.; Burns, D.; Kendall, C.; McDonnell, J.

    2002-05-01

    Water residence times were determined in 3 small watersheds in the Croton water supply area, NY. The watersheds (less than 1 km2 drainage area) have different amounts of urbanization (natural, semi-developed and fully developed), different mechanisms of runoff generation (quick flow on roads and slow flow through subsurface) and different watershed landscape characteristics (wetlands, hillslopes) . Measurements of the Oxygen-18 content of throughfall, stream water, soil water and groundwater in the saturated zone were performed bi-weekly over a period of 2 years. Mean water residence times of the stream water, soil water and groundwater were estimated using Oxygen-18 and Helium-3/Tritium isotopes. There are small but significant differences in the isotopic content of waters in each watershed, along with soil and aquifer properties as a function of the level of urbanization. Longer groundwater residence times (up to more than 2 years) were estimated in wetland zones without direct communication with streams in comparison to hillslope areas (up to more than 1 year). In highly urbanized areas, mixing of natural runoff generation processes with urbanization effects such as the influence of septic plumes results in a complex spectrum of residence times in soil waters and groundwaters. We illustrate the possibilities of using stable isotope measurements to describe small-scale complex runoff generation processes in watersheds.

  9. Teplovzdušné vytápění tělocvičny

    OpenAIRE

    Janková, Martina

    2015-01-01

    Bakalářská práce je zaměřena na návrh vzduchotechnického zařízení pro dva vybrané prostory, tělocvičnu a její hygienické zázemí, tak aby byly splněny funkční, hygienické a provozní požadavky na mikroklima sportovních objektů. Zařízení jsou navržena tak, aby byly pokryty tepelné ztráty v zimním období a zároveň dopravily požadované množství čerstvého vzduchu do daných místností. Dále jsou pokryty tepelné zisky v tělocvičně v letním období. Úvodní část práce se zabývá mikrobiálním a aerosolovým...

  10. Non-classical antigen processing pathways are required for MHC class II-restricted direct tumor recognition by NY-ESO-1-specific CD4+ T cells

    Science.gov (United States)

    Matsuzaki, Junko; Tsuji, Takemasa; Luescher, Immanuel; Old, Lloyd J.; Shrikant, Protul; Gnjatic, Sacha; Odunsi, Kunle

    2014-01-01

    Tumor antigen-specific CD4+ T cells that directly recognize cancer cells are important for orchestrating antitumor immune responses at the local tumor sites. However, the mechanisms of direct MHC class II (MHC-II) presentation of intracellular tumor antigen by cancer cells are poorly understood. We found that two functionally distinct subsets of CD4+ T cells were expanded after HLA-DPB1*04 (DP04)-binding NY-ESO-1157–170 peptide vaccination in ovarian cancer patients. While both subsets similarly recognized exogenous NY-ESO-1 protein pulsed on DP04+ target cells, only one type recognized target cells with intracellular expression of NY-ESO-1. The tumor-recognizing CD4+ T cells more efficiently recognized the short 8–9-mer peptides than the non-tumor-recognizing CD4+ T cells. In addition to endosomal/lysosomal proteases that are typically involved in MHC-II antigen presentation, several pathways in the MHC class I presentation pathways such as the proteasomal degradation and transporter-associated with antigen-processing (TAP)-mediated peptide transport were also involved in the presentation of intracellular NY-ESO-1 on MHC-II. The presentation was inhibited significantly by primaquine, a small molecule that inhibits endosomal recycling, consistent with findings that pharmacological inhibition of new protein synthesis enhances antigen presentation. Together, our data demonstrated that cancer cells selectively present peptides from intracellular tumor antigens on MHC-II by multiple non-classical antigen-processing pathways. Harnessing direct tumor-recognizing ability of CD4+ T cells could be a promising strategy to enhance antitumor immune responses in the immunosuppressive tumor microenvironment. PMID:24764581

  11. Nonclassical antigen-processing pathways are required for MHC class II-restricted direct tumor recognition by NY-ESO-1-specific CD4(+) T cells.

    Science.gov (United States)

    Matsuzaki, Junko; Tsuji, Takemasa; Luescher, Immanuel; Old, Lloyd J; Shrikant, Protul; Gnjatic, Sacha; Odunsi, Kunle

    2014-04-01

    Tumor antigen-specific CD4(+) T cells that directly recognize cancer cells are important for orchestrating antitumor immune responses at the local tumor sites. However, the mechanisms of direct MHC class II (MHC-II) presentation of intracellular tumor antigen by cancer cells are poorly understood. We found that two functionally distinct subsets of CD4(+) T cells were expanded after HLA-DPB1*04 (DP04)-binding NY-ESO-1157-170 peptide vaccination in patients with ovarian cancer. Although both subsets recognized exogenous NY-ESO-1 protein pulsed on DP04(+) target cells, only one type recognized target cells with intracellular expression of NY-ESO-1. The tumor-recognizing CD4(+) T cells more efficiently recognized the short 8-9-mer peptides than the non-tumor-recognizing CD4(+) T cells. In addition to endosomal/lysosomal proteases that are typically involved in MHC-II antigen presentation, several pathways in the MHC class I presentation pathways, such as the proteasomal degradation and transporter-associated with antigen-processing-mediated peptide transport, were also involved in the presentation of intracellular NY-ESO-1 on MHC-II. The presentation was inhibited significantly by primaquine, a small molecule that inhibits endosomal recycling, consistent with findings that pharmacologic inhibition of new protein synthesis enhances antigen presentation. Together, our data demonstrate that cancer cells selectively present peptides from intracellular tumor antigens on MHC-II by multiple nonclassical antigen-processing pathways. Harnessing the direct tumor-recognizing ability of CD4(+) T cells could be a promising strategy to enhance antitumor immune responses in the immunosuppressive tumor microenvironment.

  12. T cell immunomonitoring and tumor responses in patients immunized with a complex of cholesterol-bearing hydrophobized pullulan (CHP) and NY-ESO-1 protein.

    Science.gov (United States)

    Uenaka, Akiko; Wada, Hisashi; Isobe, Midori; Saika, Takashi; Tsuji, Kazuhide; Sato, Eiichi; Sato, Shuichiro; Noguchi, Yuji; Kawabata, Ryohei; Yasuda, Takushi; Doki, Yuichiro; Kumon, Hiromi; Iwatsuki, Keiji; Shiku, Hiroshi; Monden, Morito; Jungbluth, Achim A; Ritter, Gerd; Murphy, Roger; Hoffman, Eric; Old, Lloyd J; Nakayama, Eiichi

    2007-04-19

    We recently showed that vaccination with a complex of cholesterol-bearing hydrophobized pullulan and NY-ESO-1 protein (CHP-NY-ESO-1) elicited antibody responses in 9 of 9 patients vaccinated in a clinical trial. In this study, we performed T cell immunomonitoring and analyzed tumor responses in these patients. To evaluate CD4 and CD8 T cell responses, an IFN-gamma secretion assay was used. The assay showed low background and was sensitive for detecting antigen-specific T cells. An increase in the CD4 T cell response was observed in 2 of 2 initially sero-positive and 5 of 7 initially sero-negative patients after vaccination. An increase in the CD8 T cell response was also observed in 2 of 2 sero-positive and 5 of 7 sero-negative patients after vaccination. Analysis of peptides recognized by CD4 and CD8 T cells revealed two dominant NY-ESO-1 regions, 73-114 and 121-144. Tumor responses were observed in 3 esophageal cancer patients and a malignant melanoma patient. In 3 of 4 prostate cancer patients, prostate-specific antigen (PSA) values stabilized during the course of vaccination. The use of whole protein, containing multiple CD4 and CD8 epitopes, may be beneficial for cancer vaccines to prevent tumors from evading the immune response.

  13. SCIB2, an antibody DNA vaccine encoding NY-ESO-1 epitopes, induces potent antitumor immunity which is further enhanced by checkpoint blockade.

    Science.gov (United States)

    Xue, Wei; Metheringham, Rachael L; Brentville, Victoria A; Gunn, Barbara; Symonds, Peter; Yagita, Hideo; Ramage, Judith M; Durrant, Lindy G

    2016-06-01

    Checkpoint blockade has demonstrated promising antitumor responses in approximately 10-40% of patients. However, the majority of patients do not make a productive immune response to their tumors and do not respond to checkpoint blockade. These patients may benefit from an effective vaccine that stimulates high-avidity T cell responses in combination with checkpoint blockade. We have previously shown that incorporating TRP-2 and gp100 epitopes into the CDR regions of a human IgG1 DNA (ImmunoBody®: IB) results in significant tumor regression both in animal models and patients. This vaccination strategy is superior to others as it targets antigen to antigen-presenting cells and stimulates high-avidity T cell responses. To broaden the application of this vaccination strategy, 16 NY-ESO-1 epitopes, covering over 80% of HLA phenotypes, were incorporated into the IB (SCIB2). They produced higher frequency and avidity T cell responses than peptide vaccination. These T cells were of sufficient avidity to kill NY-ESO-1-expressing tumor cells, and in vivo controlled the growth of established B16-NY-ESO-1 tumors, resulting in long-term survival (35%). When SCIB2 was given in combination with Treg depletion, CTLA-4 blockade or PD-1 blockade, long-term survival from established tumors was significantly enhanced to 56, 67 and 100%, respectively. Translating these responses into the clinic by using a combination of SCIB2 vaccination and checkpoint blockade can only further improve clinical responses.

  14. Lack of XAGE-1b and NY-ESO-1 in metastatic lymph nodes may predict the potential survival of stage III melanoma patients.

    Science.gov (United States)

    Mori, Mariko; Funakoshi, Takeru; Kameyama, Kaori; Kawakami, Yutaka; Sato, Eiichi; Nakayama, Eiichi; Amagai, Masayuki; Tanese, Keiji

    2017-06-01

    The cancer-testis antigens (CTA) are a large family of tumor-associated antigens expressed by a variety of cancer cells and primitive germ cells of the adult testis and placenta. These tumor-restricted expressing patterns suggest that CTA would be ideal targets for tumor-specific immunotherapy. XAGE-1 is a CTA that was originally identified by computer-based screening, and four transcription variants, XAGE-1a, -1b, -1c and -1d, have been characterized to date. Although the presence of XAGE-1 transcripts has been reported in various cancers, the expression of XAGE-1b in melanoma has not been fully characterized. In this study, we performed immunohistochemical staining of XAGE-1b together with NY-ESO-1, a well-known CTA, in 113 melanoma samples obtained from 84 patients and evaluated their expression in tumor cells. The effects of expression on tumor progression and patient prognosis were analyzed. Both XAGE-1b and NY-ESO-1 were expressed at high levels in lymph node metastasis and skin metastasis samples compared with the primary site (P ESO-1). In a subgroup analysis of 22 patients with stage III lymph node metastasis, overall survival was significantly higher in the XAGE-1b and NY-ESO-1 double-negative group than in the other groups (P ESO-1 expression could have a positive influence on clinical outcome in patients with melanoma. © 2017 Japanese Dermatological Association.

  15. Chemical synthesis of a polypeptide backbone derived from the primary sequence of the cancer protein NY-ESO-1 enabled by kinetically controlled ligation and pseudoprolines.

    Science.gov (United States)

    Harris, Paul W R; Brimble, Margaret A

    2015-03-01

    The cancer protein NY-ESO-1 has been shown to be one of the most promising vaccine candidates although little is known about its cellular function. Using a chemical protein strategy, the 180 amino acid polypeptide, tagged with an arginine solubilizing tail, was assembled in a convergent manner from four unprotected peptide α-thioester peptide building blocks and one cysteinyl polypeptide, which were in turn prepared by Boc and Fmoc solid phase peptide synthesis (SPPS) respectively. To facilitate the assembly by ligation chemistries, non-native cysteines were introduced as chemical handles into the polypeptide fragments; pseudoproline dipeptides and microwave assisted Fmoc SPPS were crucial techniques to prepare the challenging hydrophobic C-terminal fragment. Three sequential kinetically controlled ligations, which exploited the reactivity between peptide arylthioesters and peptide alkylthioesters, were then used in order to assemble the more tractable N-terminal region of NY-ESO-1. The ensuing 147 residue polypeptide thioester then underwent successful final native chemical ligation with the very hydrophobic C-terminal polypeptide bearing an N-terminal cysteine affording the 186 residue polypeptide as an advanced intermediate en route to the native NY-ESO-1 protein. © 2015 Wiley Periodicals, Inc.

  16. Antibody-targeted NY-ESO-1 to mannose receptor or DEC-205 in vitro elicits dual human CD8+ and CD4+ T cell responses with broad antigen specificity.

    Science.gov (United States)

    Tsuji, Takemasa; Matsuzaki, Junko; Kelly, Marcus P; Ramakrishna, Venky; Vitale, Laura; He, Li-Zhen; Keler, Tibor; Odunsi, Kunle; Old, Lloyd J; Ritter, Gerd; Gnjatic, Sacha

    2011-01-15

    Immunization of cancer patients with vaccines containing full-length tumor Ags aims to elicit specific Abs and both CD4(+) and CD8(+) T cells. Vaccination with protein Ags, however, often elicits only CD4(+) T cell responses without inducing Ag-specific CD8(+) T cells, as exogenous protein is primarily presented to CD4(+) T cells. Recent data revealed that Ab-mediated targeting of protein Ags to cell surface receptors on dendritic cells could enhance the induction of both CD4(+) and CD8(+) T cells. We investigated in this study if these observations were applicable to NY-ESO-1, a cancer-testis Ag widely used in clinical cancer vaccine trials. We generated two novel targeting proteins consisting of the full-length NY-ESO-1 fused to the C terminus of two human mAbs against the human mannose receptor and DEC-205, both internalizing molecules expressed on APC. These targeting proteins were evaluated for their ability to activate NY-ESO-1-specific human CD4(+) and CD8(+) T cells in vitro. Both targeted NY-ESO-1 proteins rapidly bound to their respective targets on APC. Whereas nontargeted and Ab-targeted NY-ESO-1 proteins similarly activated CD4(+) T cells, cross-presentation to CD8(+) T cells was only efficiently induced by targeted NY-ESO-1. In addition, both mannose receptor and DEC-205 targeting elicited specific CD4(+) and CD8(+) T cells from PBLs of cancer patients. Receptor-specific delivery of NY-ESO-1 to APC appears to be a promising vaccination strategy to efficiently generate integrated and broad Ag-specific immune responses against NY-ESO-1 in cancer patients.

  17. 肝细胞癌NY-ESO-1基因/蛋白表达与临床病理特征及肿瘤转移的关系%Correlation of NY-ESO-1 Gene and Protein Expression to Metastasis and Clinicopathologic Features of Hepatocellular Carcinoma

    Institute of Scientific and Technical Information of China (English)

    张文敏; 肖刚; 谢丹; 张萌; 郭爱林; 文剑明

    2005-01-01

    背景与目的:NY-ESO-1属癌症-睾丸抗原家族,能激发肿瘤患者同时产生细胞免疫和体液免疫,特异杀伤肿瘤细胞,被认为是目前免疫原性最强的肿瘤抗原.本研究旨在探讨NY-ESO-1在肝细胞癌中的表达及其与肝癌临床病理特征的关系.方法:用逆转录聚合酶链反应(RT-PCR)分析62例肝癌和相应癌旁肝组织NY-ESO-1 mRNA表达;将145例肝癌石蜡组织制成芯片(132例有效),应用免疫组织化学EliVisionTM两步法观察NY-ESO-1蛋白的表达和分布.结果:NYESO-1基因在肝细胞癌中的阳性率是27.4%(17/62),肝癌伴门脉癌栓者达40%(10/25),高于不伴门脉癌栓的18.9%(7/37);NY-ESO-1蛋白主要分布在肝癌细胞胞浆,在肝癌组织芯片中的阳性率为18.9%(25/132),肝癌伴转移病变者为29.6%(16/54),不伴转移病变的阳性率仅11.5%(9/78),二者比较有显著性差异(P<0.05);NY-ESO-1基因和蛋白在HBsAg阳性肝癌标本中阳性率分别为28.3%(15/53)和19.1%(22/115),AFP≥20 μg/L标本的阳性率为29.5%(13/44)和20.7%(19/93),与HBsAg阴性和AFP正常的肝癌比较均无显著性差异(P>0.05);所有癌旁肝组织均未见NY-ESO-1的表达.结论:NY-ESO-1特异表达于肝癌细胞,在有转移病变的病例中有较高的阳性率,提示NY-ESO-1可能与肝癌病情进展和转移有关,可作为肝癌,特别是伴有转移病变的肝癌患者特异性免疫治疗的候选靶标之一;NY-ESO-1表达与HBsAg、AFP是否阳性无明显相关.

  18. NY-ESO-1 致敏树突状细胞诱导的细胞毒性 T 淋巴细胞对视网膜母细胞瘤细胞的特异性杀伤作用%The killing effect of NY-ESO-1-sensitzed dendritic cells-induced cellular toxic lymphocyte on human retinoblastoma cell

    Institute of Scientific and Technical Information of China (English)

    周学智; 周希瑗

    2012-01-01

    Background Cell immunologic therapy for retinoblastoma(RB)is becoming a hot research topic.Cancer-testis antigen is a human immunogenic protein and is used to treat some tumors.However,its effect on RB has not been investigated.Objective The present study was to discuss the antigen specific anti-tumor effect of cytotoxic T lymphocytes(CTL)induced by the cancer-testis antigen,NY-ESO-1-sensitized dendritic cells(DCs),on human RB.Methods PCR was performed to amplify target gene fragments from the NY-ESO-1 plasmids,and then the target gene fragments were digested with the restriction enzymes SalI and EcoRI.Harvested fragments were inserted into the pDC316 plasmid to construct the recombinant plasmid pDC316/NY-ESO-1.The expression of NY-ESO-1 protein in human RB cells strain,HXO-RB44,was detected by immunofluorescence and Western blot.Monocytes were isolated from 60 ml of peripheral blood from a healthy donor using Ficoll density-gradient centrifugation with a cell density of 1 × 107/ml.DCs isolated from blood were stimulated with recombinant human granulocyte-macrophage colony stimulating factor(rhGM-CSF)and recombinant human interleukin-4(rhIL-4).The recombinant plasmid pDC316/NY-ESO-1 was transfected into DCs and the DCs were co-cultured with T lymphocytes.The resultant CTL were used as effector cells.The growth of the CTL was detected by MTT assay.The CTL were then added into the growth medium used for culturing HXO-RB44 cells and the vitality of the HXO-RB44 cells was assayed by MTT assay.Results The sequence of the cloned DNA fragment of the recombinant plasmid pDC316/NY-ESO-1 was conforms with the sequence of the NY-ESO-1 gene.The expression of the NY-ESO-1 protein in HXO-RB44 cells was tested by immunofluorescence and Western blot.DCs were successfully induced with rhIL-4 and rhGM-CSF from PBMC.The recombinant expression plasmid pDC316/NY-ESO-1 was successfully transferred into DCs.These DCs had high expression of surface molecules such as HLA-DR(42.1%),CD80

  19. Atmospheric dry deposition of trace elements measured around the urban and industrially impacted NY-NJ harbor

    Science.gov (United States)

    Yi, Seung-Muk; Totten, Lisa A.; Thota, Sathyapriya; Yan, Shu; Offenberg, John H.; Eisenreich, Steven J.; Graney, Joseph; Holsen, Thomas M.

    Long-term direct trace metal dry deposition measurements were made during 2001 and 2002 at two sites near the NY/NJ Harbor Estuary in New Jersey: Jersey City (JC) and New Brunswick (NB). In addition intensive dry deposition and atmospheric size distribution (ASD) measurements were made between September 2001 and June 2002. Dry deposition samples were obtained using modified MIC-B wet deposition samplers which deployed knife-edge surrogate surfaces during dry periods. ASDs were measured with micro-orifice uniform deposit impactors (MOUDI) and coarse particle rotary impactors (CPRI). The fluxes of trace metals were found to be highest at the JC site and lowest at the NB site. The dry deposition fluxes ranged from 0.030 (Cd) to 3800 (Al) μg m -2 d -1 and 0.14 (Cd) to 8100 (Al) μg m -2 d -1at NB and JC sites, respectively. Metals of crustal origin were found mostly in the coarse particle fraction and anthropogenic metals were found mostly in the fine particle fraction. For the intensive experiments, overall dry deposition velocities of several trace metals were determined by dividing measured fluxes by measured concentrations in different particle size ranges. For most of the elements, the flux was highly correlated with PM 10 and coarse particle concentrations. The overall dry deposition velocities of crustal elements (Al and Mg) varied from 3.7 to 8.7 cm s -1 with correlation coefficients ranging from 0.50 to 0.84. The best-fit dry deposition velocities for anthropogenic elements (Cr, Mn, Zn, and Pb; Cu, Mo, and Ba; V and Ni) varied more than those for crustal elements (0.52-25 vs. 3.7-8.7 cm s -1). The dry deposition velocities for most elements obtained using three size ranges were statistically significant with R2 values ranging from 0.37 to 0.84 except for V and Ni (0.12).

  20. Definition of key variables for the induction of optimal NY-ESO-1-specific T cells in HLA transgene mice.

    Science.gov (United States)

    Johannsen, Alexandre; Genolet, Raphaël; Legler, Daniel F; Luther, Sanjiv A; Luescher, Immanuel F

    2010-09-15

    An attractive treatment of cancer consists in inducing tumor-eradicating CD8(+) CTL specific for tumor-associated Ags, such as NY-ESO-1 (ESO), a strongly immunogenic cancer germ line gene-encoded tumor-associated Ag, widely expressed on diverse tumors. To establish optimal priming of ESO-specific CTL and to define critical vaccine variables and mechanisms, we used HLA-A2/DR1 H-2(-/-) transgenic mice and sequential immunization with immunodominant DR1- and A2-restricted ESO peptides. Immunization of mice first with the DR1-restricted ESO(123-137) peptide and subsequently with mature dendritic cells (DCs) presenting this and the A2-restriced ESO(157-165) epitope generated abundant, circulating, high-avidity primary and memory CD8(+) T cells that efficiently killed A2/ESO(157-165)(+) tumor cells. This prime boost regimen was superior to other vaccine regimes and required strong Th1 cell responses, copresentation of MHC class I and MHC class II peptides by the same DC, and resulted in upregulation of sphingosine 1-phosphate receptor 1, and thus egress of freshly primed CD8(+) T cells from the draining lymph nodes into circulation. This well-defined system allowed detailed mechanistic analysis, which revealed that 1) the Th1 cytokines IFN-gamma and IL-2 played key roles in CTL priming, namely by upregulating on naive CD8(+) T cells the chemokine receptor CCR5; 2) the inflammatory chemokines CCL4 (MIP-1beta) and CCL3 (MIP-1alpha) chemoattracted primed CD4(+) T cells to mature DCs and activated, naive CD8(+) T cells to DC-CD4 conjugates, respectively; and 3) blockade of these chemokines or their common receptor CCR5 ablated priming of CD8(+) T cells and upregulation of sphingosine 1-phosphate receptor 1. These findings provide new opportunities for improving T cell cancer vaccines.

  1. Trypanosoma cruzi adjuvants potentiate T cell-mediated immunity induced by a NY-ESO-1 based antitumor vaccine.

    Science.gov (United States)

    Junqueira, Caroline; Guerrero, Ana Tereza; Galvão-Filho, Bruno; Andrade, Warrison A; Salgado, Ana Paula C; Cunha, Thiago M; Ropert, Catherine; Campos, Marco Antônio; Penido, Marcus L O; Mendonça-Previato, Lúcia; Previato, José Oswaldo; Ritter, Gerd; Cunha, Fernando Q; Gazzinelli, Ricardo T

    2012-01-01

    Immunological adjuvants that induce T cell-mediate immunity (TCMI) with the least side effects are needed for the development of human vaccines. Glycoinositolphospholipids (GIPL) and CpGs oligodeoxynucleotides (CpG ODNs) derived from the protozoa parasite Trypanosoma cruzi induce potent pro-inflammatory reaction through activation of Toll-Like Receptor (TLR)4 and TLR9, respectively. Here, using mouse models, we tested the T. cruzi derived TLR agonists as immunological adjuvants in an antitumor vaccine. For comparison, we used well-established TLR agonists, such as the bacterial derived monophosphoryl lipid A (MPL), lipopeptide (Pam3Cys), and CpG ODN. All tested TLR agonists were comparable to induce antibody responses, whereas significant differences were noticed in their ability to elicit CD4(+) T and CD8(+) T cell responses. In particular, both GIPLs (GTH, and GY) and CpG ODNs (B344, B297 and B128) derived from T. cruzi elicited interferon-gamma (IFN-γ) production by CD4(+) T cells. On the other hand, the parasite derived CpG ODNs, but not GIPLs, elicited a potent IFN-γ response by CD8(+) T lymphocytes. The side effects were also evaluated by local pain (hypernociception). The intensity of hypernociception induced by vaccination was alleviated by administration of an analgesic drug without affecting protective immunity. Finally, the level of protective immunity against the NY-ESO-1 expressing melanoma was associated with the magnitude of both CD4(+) T and CD8(+) T cell responses elicited by a specific immunological adjuvant.

  2. Demographic Profiles, Mercury, Selenium, and Omega-3 Fatty Acids in Avid Seafood Consumers on Long Island, NY.

    Science.gov (United States)

    Monastero, Rebecca; Karimi, Roxanne; Silbernagel, Susan; Meliker, Jaymie

    2016-02-01

    Seafood consumption is known to confer nutritional benefits and risks from contaminant exposure. Avid seafood consumers are neither well-characterized with regard to their demographic profile nor their underlying risk-benefit profile. Contaminants [e.g., mercury (Hg)] and nutrients [e.g., selenium (Se), omega-3 fatty acids] are prevalent in some seafood. Participants (N = 285) recruited on Long Island, NY, completed food frequency and health questionnaires and received blood draws analyzed for Hg, omega-3s, and Se. Participants were categorized based on frequency and type of seafood consumption. Logistic regression analyses evaluated relationships between seafood consumption and demographics, and were age- and sex-adjusted. t tests assessed relationships between seafood consumption patterns and biomarkers Hg, omega-3s, and Se. Consumption of both tuna and salmon was associated with older age: those aged 55-75 and over 75 years old were more likely than participants aged 18-34 to eat tuna and salmon (OR 2.27; 95% CI 1.05, 4.89 and OR 3.67; 95% CI 1.20, 11.20, respectively). Males were less likely than females to eat fish other than tuna or salmon (OR 0.58; 95% CI 0.34, 0.97). Caucasians were more likely to consume tuna (OR 0.31; 95% CI 0.10, 0.96) or salmon and tuna (OR 0.34; 95% CI 0.12, 0.91), while non-Caucasians were more likely to consume other fish types (OR 2.73; 95% CI 1.45, 5.12). Total blood Hg was associated with weekly consumption of any type of fish (p = 0.01) and with salmon and tuna consumption (p = 0.01). Salmon was associated with plasma omega-3s (p = 0.01). Se was not associated with fish intake categories. Risk communicators can use these findings to influence seafood preferences of different demographic groups.

  3. Temporal Variation in the Stable Isotopic Composition of Water and Sediment in Seneca Lake, NY (USA): Implications for Paleoclimate Reconstructions

    Science.gov (United States)

    Curtin, T.; Finkelstein, D. B.; Gunn, P.

    2014-12-01

    Paleoclimatic studies often use stratigraphic changes in the oxygen isotope composition (δ18O) of lacustrine carbonate to infer changes in water temperature and/or the δ18O of lake water. To better understand the environmental factors that influence the isotopic composition of lacustrine carbonates and the assumptions that need to be made to infer past changes in climate, we undertook a four-year study of the water chemistry and sediment trap material in Seneca Lake (NY, USA), a large, monomictic, glacial, hardwater lake. Sediment trap material collected weekly between May 2009 and 2013 together with isotopic monitoring of surface and bottom water allow for analysis of the controls on calcite precipitation in the lake. We show that calcite mainly accumulates in traps July through September when the lake water column is stratified and epilimnetic water temperature exceeds 20°C. Up to ~70% of the sediment is comprised of calcite during summer and only ~10% of the sediment is calcite during autumn, winter, and spring. The δ18O of Seneca Lake epilimnetic water varies by only ~0.6 ‰ throughout the year whereas the δ18O of bulk carbonate varies by as much as 2.4‰. As calcite precipitates in the eplimnion, the δ18O declines. Likely due to the large volume and residence time of water, the δ18O of Seneca Lake water appears to track changes in temperature. Our temperature reconstruction using the δ18O of calcite and epilimnetic lake water reveals that calcite was a reasonable proxy for lake surface temperature from July through September. During the remainder of the year, reconstructed temperatures exceed actual temperatures by as much as 18°C. Sediment resuspension (including calcite) during isothermal conditions may explain why calcite is a poor predictor of lake surface temperature in late autumn though spring. The δ18O of calcite in this lake records summer temperatures rather than year-round conditions.

  4. Physical properties of the arctic summer aerosol particles in relation to sources at Ny-Alesund, Svalbard

    Indian Academy of Sciences (India)

    C G Deshpande; A K Kamra

    2014-02-01

    Measurements of the number concentration and size distribution of aerosol particles in the size range of 0.5–20 m diameter were made with an aerodynamic particle sizer at an Arctic site at Ny-Alesund, Svalbard in August–September 2007 during the International Polar Year 2007–2008. Data are analyzed to study the aerosol number concentration–wind speed relationships. The sea-salt particles of marine origin generated within the Arctic circle are identified as the main source of the Arctic summer aerosols. Total number concentration of aerosol particles increases with increase in wind speed, the increase being more when winds from open leads over the oceanic sector are reaching the station as compared to when winds from pack ice in other directions are reaching the station. The larger increase with winds from the oceanic sector is attributed to the enhanced bubble-breaking activity and increased entrainment of dimethyl sulphide particles at the sea surface. Although, the increase in total aerosol number concentration associated with the winds from the oceanic sector is spread over the whole range of particle sizes, the increase in coarse mode particles is more prominent than that in the accumulation mode particles. The age of airmass over pack ice is also an important factor to determine the aerosol concentration over the Arctic region. The process of rainout/washout of the aerosol particles due to drizzle/snowfall is an effective sink mechanism in the Arctic environment. The aerosol particle concentration starts decreasing within a few minutes from the start of these events but requires a few hours to restore to the normal background aerosol level after the end of event.

  5. Air pollution at Rochester, NY: Long-term trends and multivariate analysis of upwind SO2 source impacts.

    Science.gov (United States)

    Emami, Fereshteh; Masiol, Mauro; Hopke, Philip K

    2017-09-11

    There have been many changes in the air pollutant sources in the northeastern United States since 2001. To assess the effect of these changes, trend analyses of the monthly average values were performed on PM2.5 and its components including major ions, elemental carbon (EC), organic carbon (OC), and gaseous pollutant concentrations measured between 2001 (in some cases 1999) and 2015 at the NYS Department of Environmental Conservation sites in Rochester, NY. Mann-Kendall regression with Sen's slope was applied to estimate the trends and seasonality. Using piecewise regression, significant reductions in the air pollution of Rochester area were observed between 2008 and 2010 when a 260MW coal-fired power plant was decommissioned, new heavy-duty diesel trucks had to be equipped with catalytic regenerator traps, and the economic recession that began in 2008 reduced traffic and other activities. The monthly average PM2.5 mass showed a downward trend (-5μg/m(3); -41%) in Rochester between 2001 and 2015. This change is largely due to reductions in particulate sulfate that showed a 65% decrease. The sulfate concentrations were compared to changes in SO2 emissions in seventeen upwind source domains, and other systematic changes by multivariate linear regression. Selectivity ratio obtained from target projection discriminated the most important source domains that are SO2 emissions from Georgia for winter, North Carolina for transition (spring and fall) and Ohio along with other influences for summer. North Carolina and Michigan were identified as the main sources for entire period. These observations suggest that any further reductions in the specified regional SO2 emissions would result in a proportional decrease in sulfate in Rochester. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  6. Trypanosoma cruzi adjuvants potentiate T cell-mediated immunity induced by a NY-ESO-1 based antitumor vaccine.

    Directory of Open Access Journals (Sweden)

    Caroline Junqueira

    Full Text Available Immunological adjuvants that induce T cell-mediate immunity (TCMI with the least side effects are needed for the development of human vaccines. Glycoinositolphospholipids (GIPL and CpGs oligodeoxynucleotides (CpG ODNs derived from the protozoa parasite Trypanosoma cruzi induce potent pro-inflammatory reaction through activation of Toll-Like Receptor (TLR4 and TLR9, respectively. Here, using mouse models, we tested the T. cruzi derived TLR agonists as immunological adjuvants in an antitumor vaccine. For comparison, we used well-established TLR agonists, such as the bacterial derived monophosphoryl lipid A (MPL, lipopeptide (Pam3Cys, and CpG ODN. All tested TLR agonists were comparable to induce antibody responses, whereas significant differences were noticed in their ability to elicit CD4(+ T and CD8(+ T cell responses. In particular, both GIPLs (GTH, and GY and CpG ODNs (B344, B297 and B128 derived from T. cruzi elicited interferon-gamma (IFN-γ production by CD4(+ T cells. On the other hand, the parasite derived CpG ODNs, but not GIPLs, elicited a potent IFN-γ response by CD8(+ T lymphocytes. The side effects were also evaluated by local pain (hypernociception. The intensity of hypernociception induced by vaccination was alleviated by administration of an analgesic drug without affecting protective immunity. Finally, the level of protective immunity against the NY-ESO-1 expressing melanoma was associated with the magnitude of both CD4(+ T and CD8(+ T cell responses elicited by a specific immunological adjuvant.

  7. Morphochemical characteristics and mixing state of long range transported wildfire particles at Ny-Ålesund (Svalbard Islands)

    Science.gov (United States)

    Moroni, Beatrice; Cappelletti, David; Crocchianti, Stefano; Becagli, Silvia; Caiazzo, Laura; Traversi, Rita; Udisti, Roberto; Mazzola, Mauro; Markowicz, Krzysztof; Ritter, Christoph; Zielinski, Tymon

    2017-05-01

    A prolonged and exceptionally intense air mass advection event transporting biomass burning aerosols generated in Alaska affected Ny-Ålesund in the mid of July 2015. This paper reports the morphochemical characteristics and mixing state of individual aerosol particles collected during the event. To this aim aerosol samples were collected on nucleopore polycarbonate membrane filters using a DEKATI 12-stage low volume impactor and analyzed by scanning electron microscopy (SEM) techniques. Results of SEM investigations depict a complex aerosol characterized by an external mixing between a main part of carbonaceous organic particles (tar balls and organic particles), lower ammonium sulfate and minor potassium chloride and mineral dust amounts. The carbonaceous particles are spherical to slightly elongated and the organic particles show an internal mixing of low density organics and/or ammonium sulfate upon denser nuclei. Most particles are in the accumulation mode size range although the size and the morphology of the chloride and the sulfate salts evidence the growth of these species both in the air and upon the sampling membranes. Individual particle analyses were complemented by aerosol size distribution (Aerodynamic Particle Sizer, Scanning Mobility Particle Sizer) and optical (Particle Soot Absorption Photometer, nephelometer) measurements at ground level in order to retrieve the optical and radiative properties of the aerosol in the atmosphere and to predict the fate and behaviour of particles upon deposition at ground level. Individual particle analyses were also compared with bulk chemical analyses on daily sampling filters and back-trajectory analyses of the air mass movement in order to enucleate distinct sources of the aerosol during the long range transport.

  8. Trypanosoma cruzi Adjuvants Potentiate T Cell-Mediated Immunity Induced by a NY-ESO-1 Based Antitumor Vaccine

    Science.gov (United States)

    Junqueira, Caroline; Guerrero, Ana Tereza; Galvão-Filho, Bruno; Andrade, Warrison A.; Salgado, Ana Paula C.; Cunha, Thiago M.; Ropert, Catherine; Campos, Marco Antônio; Penido, Marcus L. O.; Mendonça-Previato, Lúcia; Previato, José Oswaldo; Ritter, Gerd; Cunha, Fernando Q.; Gazzinelli, Ricardo T.

    2012-01-01

    Immunological adjuvants that induce T cell-mediate immunity (TCMI) with the least side effects are needed for the development of human vaccines. Glycoinositolphospholipids (GIPL) and CpGs oligodeoxynucleotides (CpG ODNs) derived from the protozoa parasite Trypanosoma cruzi induce potent pro-inflammatory reaction through activation of Toll-Like Receptor (TLR)4 and TLR9, respectively. Here, using mouse models, we tested the T. cruzi derived TLR agonists as immunological adjuvants in an antitumor vaccine. For comparison, we used well-established TLR agonists, such as the bacterial derived monophosphoryl lipid A (MPL), lipopeptide (Pam3Cys), and CpG ODN. All tested TLR agonists were comparable to induce antibody responses, whereas significant differences were noticed in their ability to elicit CD4+ T and CD8+ T cell responses. In particular, both GIPLs (GTH, and GY) and CpG ODNs (B344, B297 and B128) derived from T. cruzi elicited interferon-gamma (IFN-γ) production by CD4+ T cells. On the other hand, the parasite derived CpG ODNs, but not GIPLs, elicited a potent IFN-γ response by CD8+ T lymphocytes. The side effects were also evaluated by local pain (hypernociception). The intensity of hypernociception induced by vaccination was alleviated by administration of an analgesic drug without affecting protective immunity. Finally, the level of protective immunity against the NY-ESO-1 expressing melanoma was associated with the magnitude of both CD4+ T and CD8+ T cell responses elicited by a specific immunological adjuvant. PMID:22567144

  9. A reversible bipolar WORM device based on AlOxNy thin film with Al nano phase embedded

    Science.gov (United States)

    Zhu, W.; Li, J.; Zhang, L.; Hu, X. C.

    2017-03-01

    An Al-rich AlOxNy thin film based reversible Write-Once-Read-Many-Times (WORM) memory device with MIS structure could transit from high resistance state (HRS, ∼1011 Ω) to low resistance state (LRS, ∼105 Ω) by sweeping voltage up to ∼20 V. The first switching could be recorded as writing process for WORM device which may relate to conductive path are formed through the thin film. The conductive path should be formed by both Al nano phase and oxygen vacancies. Among of them, Al nano phases are not easy to move, but oxygen vacancies could migrate under high E-field or at high temperature environment. Such conductive path is not sensitive to charging effect after it formed, but it could be broken by heating effect, which may relate to the migration of excess Al ions and oxygen vacancies at high temperature. After baking LRS (ON state) WORM device at 200 °C for 2 min, the conductivity will decrease to HRS which indicates conductive path is broken and device back to HRS (OFF state) again. This phenomenon could be recorded as recovery process. Both writing and recovery process related to migration of oxygen vacancies and could be repeated over 10 times in this study. It also indicates that there is no permanent breakdown occurred in MIS structured WORM device operation. We suggest that this conductive path only can be dissolved by a temperature sensitive electro-chemical action. This WORM device could maintain at LRS over 105 s with on-off ratio over 4 orders.

  10. Effective inhibition of melanoma tumorigenesis and growth via a new complex vaccine based on NY-ESO-1-alum-polysaccharide-HH2

    Science.gov (United States)

    2014-01-01

    Background A safe and effective adjuvant plays an important role in the development of a vaccine. However, adjuvants licensed for administration in humans remain limited. Here, for the first time, we developed a novel combination adjuvant alum-polysaccharide-HH2 (APH) with potent immunomodulating activities, consisting of alum, polysaccharide of Escherichia coli and the synthetic cationic innate defense regulator peptide HH2. Methods The adjuvant effects of APH were examined using NY-ESO-1 protein-based vaccines in prophylactic and therapeutic models. We further determined the immunogenicity and anti-tumor effect of NY-ESO-1-APH (NAPH) vaccine using adoptive cellular/serum therapy in C57/B6 and nude mice. Cell-mediated and antibody-mediated immune responses were evaluated. Results The APH complex significantly promoted antigen uptake, maturation and cross-presentation of dendritic cells and enhanced the secretion of TNF-α, MCP-1 and IFN-γ by human peripheral blood mononuclear cells compared with individual components. Vaccination of NAPH resulted in significant tumor regression or delayed tumor progression in prophylactic and therapeutic models. In addition, passive serum/cellular therapy potently inhibited tumor growth of NY-ESO-1-B16. Mice treated with NAPH vaccine produced higher antibody titers and greater antibody-dependent/independent cellular cytotoxicity. Therefore, NAPH vaccination effectively stimulated innate immunity, and boosted both arms of the adaptive humoral and cellular immune responses to suppress tumorigenesis and growth of melanoma. Conclusions Our study revealed the potential application of APH complex as a novel immunomodulatory agent for vaccines against tumor refractory and growth. PMID:25070035

  11. Research in particle physics. [Dept. of Physics, Boston Univ

    Energy Technology Data Exchange (ETDEWEB)

    Whitaker, Scott J.

    1992-09-01

    Research accomplishments and current activities of Boston University researchers in high energy physics are presented. Principal areas of activity include the following: detectors for studies of electron[endash]positron annihilation in colliding beams; advanced accelerator component design, including the superconducting beam inflector, electrostatic quadrupoles, and the electrostatic muon kicker''; the detector for the MACRO (Monopole, Astrophysics, and Cosmic Ray Observatory) experiment; neutrino astrophysics and the search for proton decay; theoretical particle physics (electroweak and flavor symmetry breaking, hadron collider phenomenology, cosmology and astrophysics, new field-theoretic models, nonperturbative investigations of quantum field theories, electroweak interactions); measurement of the anomalous magnetic moment of the muon; calorimetry for the GEM experiment; and muon detectors for the GEM experiment at the Superconducting Super Collider.

  12. Experimental particle physics. [Dept. of Physics, Drexel Univ

    Energy Technology Data Exchange (ETDEWEB)

    Steinberg, R.I.; Lane, C.E.

    1992-09-01

    The goals of this research are the experimental testing of fundamental theories of physics beyond the standard model and the exploration of cosmic phenomena through the techniques of particle physics. We are working on the MACRO experiment, which employs a large-area underground detector to search fore grand unification magnetic monopoles and dark matter candidates and to study cosmic ray muons as well as low- and high-energy neutrinos; the Chooz experiment to search for reactor neutrino oscillations at a distance of 1 km from the source; a new proposal (the Perry experiment) to construct a one-kiloton liquid scintillator in the Fairport, Ohio underground facility IMB to study neutrino oscillations with a 13 km baseline; and development of technology for improved liquid scintillators and for very-low-background materials in support of the MACRO and Perry experiments and for new solar neutrino experiments.

  13. Annual progress report 2000. Wind Energy and Atmospheric Physics Dept.

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, S.E.; Skrumsager, B. [eds.

    2001-05-01

    The report describes the work of the Wind Energy and Atmospheric Physics Department at Risoe National Laboratory in 2000. The research of the department aims to develop new opportunities in the exploitation of wind energy and to map and alleviate atmospheric aspects of environmental problems. The expertise of the department is utilised in commercial activities such as wind turbine testing and certification, training programmes, courses and consultancy services to industry, authorities and Danish and international organisations on wind energy and atmospheric environmental impact. A summary of the department's activities in 2000 is shown, including lists of publications, lectures, committees and staff members. (au)

  14. Dept. o/Zoology, University 0/ Durban-Westville

    African Journals Online (AJOL)

    Since their age was unknown the embryos were roughly classUied ... between the cells and matrix of the cartilage or its precursors and the ..... The stem of the ceratohyal becomes, in comparison with the columella auris, relatively long.

  15. Original Article Moges Abay* *Psychology Dept. Educ. Faculty, JU ...

    African Journals Online (AJOL)

    dell

    students with their social interactions with their family members, hearing peers, and teachers and .... selected schools for the study, Hermata,. Mendera .... qualification. Sex. Frequency. Service Years. One year training in teaching. Diploma in ...

  16. Deexcitation processes in nuclear reactions. [Dept. of Chemistry, Purdue Univ

    Energy Technology Data Exchange (ETDEWEB)

    Porile, N.T.

    1992-09-01

    During the past year, our research program has involved continuing analysis of Fermilab E-735, search for quark-gluon plasma (QGP) in {bar p}-p collisions; continuing study of target fragments produced in the interaction of copper with intermediate-energy heavy ions; an exclusive study of multifragmentation using reverse kinematics at the Bevalac; and detector development for the STAR detector at RHIC.

  17. Education Dept. May Ban Exclusive Deals between Colleges and Lenders

    Science.gov (United States)

    Burd, Stephen

    2007-01-01

    This article discusses exclusive deals between colleges and student-loan providers and the U.S. Education Department's initial move to solve the conflict. The Higher Education Act, which governs most federal student-aid programs, prohibits colleges from requiring their students to borrow from a specific bank or student-loan company. The law does…

  18. Dept. of Community Medicine, Ahmadu Bello University, Zaria, Nigeria.

    African Journals Online (AJOL)

    Fisher did not stop there but graded the strength of evidence against null hypothesis. .... which null hypothesis is true will result in P < 0.05!. Finally, with these and ..... odds ratio) null hypothesis is rejected at 0.05 levels. 6. Finally, the use of CIs ...

  19. montauk_ny.grd

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NGDC builds and distributes high-resolution, coastal digital elevation models (DEMs) that integrate ocean bathymetry and land topography to support NOAA's mission to...

  20. Ny svensk teaterhistoria

    DEFF Research Database (Denmark)

    Kuhlmann, Annelis

    2009-01-01

    Review about the New Swedish Theatre History, edited by Sven Åke Heed and Tomas Forser. The book is in three volumes. Focus is on performativity and theatrical appearances within spoken theatre, opera, ballet, musicals, free dance (a Swedish notion of modern dance).......Review about the New Swedish Theatre History, edited by Sven Åke Heed and Tomas Forser. The book is in three volumes. Focus is on performativity and theatrical appearances within spoken theatre, opera, ballet, musicals, free dance (a Swedish notion of modern dance)....

  1. Ny servicerobot til landbruget

    DEFF Research Database (Denmark)

    Sørensen, Torben

    2005-01-01

    På DTU er der udviklet en servicerobot til landbruget – den autonome landbrugshjælper. Robotten har højpræcisions GPS (RTK), encodere, kompas og tiltsensorer for at kunne positionere sig selv og følge en specificeret rute, endvidere har robotten firhjulstræk og styring på alle fire hjul, hvilket...

  2. FLOODPLAIN, JEFFERSON COUNTY, NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — The Floodplain Mapping/Redelineation study deliverables depict and quantify the flood risks for the study area. The primary risk classifications used are the...

  3. ORTHOIMAGERY, Otsego County, NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Digital orthographic imagery datasets contain georeferenced images of the Earth's surface, collected by a sensor in which object displacement has been removed for...

  4. Mod en ny informationsret

    DEFF Research Database (Denmark)

    Andersen, Mads Bryde

    2009-01-01

    Artiklen fremlægger og diskuterer nogle af de tanker, der finder udtryk i afhandlingen »De informationsretlige grundsætninger«, som professor Henrik Udsen netop har forsvaret for den juridiske doktorgrad. I senere artikler i samme tidsskrift (s. 383 ff., s. 389 ff., s. 392 ff., s. 397 ff. og s. 400...... ff.)behandles andre elementer af afhandlingen....

  5. Flexploitation - det ny precariat

    DEFF Research Database (Denmark)

    Ekman, Susanne

    2009-01-01

    De moderne, kreative vidensarbejdere lider – som proletariatet i den tidlige industrialisme – af manglende klassebevidsthed. Deres fleksibilitet, mobilitet og individualisme blokerer for erkendelsen af fælles vilkår....

  6. A statistical study of dayside diffuse aurora observed at Yellow River Station in Ny-Ålesund, Svalbard

    Science.gov (United States)

    Han, D.; Hu, Z.; Hu, H.; Yang, H.; Huang, D. H.; Chen, X.

    2014-12-01

    Chinese Yellow River Station (YRS), at Ny-Alesund, Svalbard is one of the few stations that can make longtime optical auroral observation at the cusp latitude in the dayside during the boreal winter season on the Earth. Since November 2003, an optical observation system consisting of three identical all-sky imagers supplied with the narrow band filters centered at 427.8, 557.5 and 630.0nm, has been installed at YRS, and the continuous observations providing us with an unprecedented opportunity to investigate some new properties of dayside aurora. Optical diffuse auroras are normally observed at the sub-aurora latitude in nightside on the ground. However, by using 7-year continuous observations obtained at YRS, we found that the diffuse auroras are very frequently observed on the dayside at the high latitude, i.e., near the cusp. We classified the dayside diffuse aurora into four primary categories. They are (1) Veiling Diffuse Aurora, which has no obvious morphological structure and clear boundaries, sometimes likes a thick cloud cover the entire sky, (2) Patch Diffuse Aurora, which is an aurora patch normally in ~10-100km in width and usually owns pulsating property; (3) Diffuse Arcs, which looks like an arc but is apparently different from the discrete auroral arcs in morphology, and (4) Pulsating Aurora, occurring with sparkling forms. We statistical studied how the occurrence of the four types of dayside diffuse auroras depends on the local time, magnetic activity, and solar wind conditions. We found that the occurrence of each type of dayside diffuse aurora shows a unimodal distribution with magnetic local time and their peaks are mostly appeared near the magnetic local noon. We also noted that ~92.2% of the dayside diffuse auroras is observed under low magnetic activity, i.e., Kp ≤ 3, and the dayside diffuse auroras prefer to be observed under IMF By0. Although previous studies suggested that the dayside diffuse aurora should be caused by wave scattering of

  7. Impact of climate warming on snow processes in Ny-Ålesund, a polar maritime site at Svalbard

    Science.gov (United States)

    López-Moreno, J. I.; Boike, J.; Sanchez-Lorenzo, A.; Pomeroy, J. W.

    2016-11-01

    The impact of observed changes in air temperature and precipitation from 1969 to 2013 and climate projections for 2050 and 2080 at Ny-Ålesund, an arctic research station on Spitzbergen Island in the Svalbard Archipelago on snow hydrological processes, were analyzed using snow accumulation and ablation algorithms in the physically based Cold Regions Hydrological Modelling platform (CRHM). The climate projections were obtained from phase 5 of the Coupled Model Intercomparison Project (CMIP5), with a focus on the snow-dominated period (October to June). To identify the potential effects of increasing temperature and precipitation, a model sensitivity analysis (1 °C to 5 °C), with and without a 25% increase in precipitation, was run on CRHM snow processes. The results indicated that the greatest observed warming was during the early snow season (October-February), with increases of 0.8 and 0.9 °C decade- 1 for maximum (Tmax) and minimum (Tmin) temperatures, respectively. There was also a significant increase in annual and winter precipitation (24 mm decade- 1). The late snow season (March-June) also had a marked increase in temperature (0.5 and 0.69 °C decade- 1 for Tmax and Tmin respectively), but no significant change in precipitation. These changes lead to a significant increase in the number of days with rainfall rather than snowfall. The sensitivity analysis indicated that mean snow water equivalent snowpack will decrease by 10.2% (early snow season) and 11.1% (late snow season) per degree of increased air temperature. For each degree of temperature increase, the modelled peak snow-water-equivalent (SWE) declined by 6.9%, duration of snowpack declined 11 days, and the number of days with rain increased 43% for the early snow season and 12.8% for the late snow season. A warmer climate also leads to markedly decreased surface snow sublimation and the fraction of snowfall eroded and transported by blowing snow. For most snowpack parameters analyzed, the response

  8. Extreme differentiation in single samples from the olivine-rich zone of the Palisades Sill, NY and NJ

    Science.gov (United States)

    Chau, K. X.; Sen, G.; Naslund, H. R.

    2007-12-01

    The Palisades Sill, NY and NJ, has long been the focus of much attention due to its potential contributions to the understanding of igneous differentiation. Many early authors, including N.L. Bowen, thought of the intrusion as an ideal example of crystal settling because of an olivine-rich zone that occurs close to the base of the sill. Later investigations demonstrated that at least three geochemically distinct batches of magma were emplaced at different levels within this body, one of which produced the olivine-rich zone. Here we report the results of our detailed electron probe study of 27 olivine grains, 134 plagioclase grains, and 181 pyroxene grains from four samples of the olivine-rich zone. In one of the rocks plagioclase ranges from An84 (large grain; core) to An0.5 (interstitial plagioclase), whereas olivine ranges from Fo81 - Fo69. These ranges are comparable to our other samples. The plagioclase range is far greater than what is shown by the Skaergaard (An25 - An69) and Bushveld (An30 - An80) intrusions. Though the sill has long been known to be vertically differentiated, such extreme differentiation in individual thumb-sized samples was not expected. The implication is that interstitial melt pockets were so well enclosed (i.e., zero permeability) by the surrounding crystals that they were sealed off from further communication with larger volumes of melt that may have existed at a higher levels in the sill at that time. That is, compaction and associated filter pressing was not efficient in removing the interstitial melt. It is unlikely that such a situation could occur if the olivine-rich zone formed early from a large body of magma because the load pressure of the overlying crystals and melt would be too great to avoid compaction. A better explanation is that the olivine-rich zone was emplaced as a separate magma batch (as suggested by several previous workers based on bulk rock geochemistry) in a mostly solidified magma-crystal mush. In this setting

  9. Atmospheric boundary layer characteristics based on the observations at the Climate Change Tower in Ny Alesund( Svalbard).

    Science.gov (United States)

    Schiavon, Mario; Mazzola, Mauro; Lupi, Angelo; Drofa, Oxana; Tampieri, Francesco; Pelliccioni, Armando; Choi, Taejin; Vitale, Vito; Viola, Angelo P.

    2017-04-01

    At high latitudes, the Atmospheric Boundary Layer ( ABL) is often characterized by extremely stable vertical stratification since the surface radiative cooling determines inversions in temperature profiles especially during the polar night over land, ice and snow surfaces. Improvements are required in the theoretical understanding of the turbulent behavior of the high-latitude ABL. The parameterizations of surface-atmosphere exchanges employed in numerical weather prediction and climate models have also to be tested in the Arctic area. Moreover, the boundary layer structure and dynamics influence the vertical distribution of aerosol. The main issue is related to the height of PBL: the question is whether some decoupling occurs between the surface layer and the atmosphere aloft when the PBL is shallow or the mechanical mixing due to the synoptic circulation provides an overall vertical homogeneity of the concentration of the aerosol irrespective of the stability conditions. In this aim, the work investigates the features of the high-latitude ABL with particular attention to its vertical structure, the relationships among the main turbulent statistics (in a similarity approach) and their variation with the ABL state. The used data refer to measurements collected since 2012 to 2016 by slow and fast response sensors deployed at the 34 m high Amundsen-Nobile Climate Change Tower (CCT) installed at Ny-Ålesund, Svalbard. Data from four conventional Young anemometers and Väisäla thermo-hygrometers at 2, 4.8, 10.3 and 33.4 m a.g.l., alternated by three lined up sonic anemometers at 3.7, 7.5 and 21 m a.g.l., are used in the analysis. The presented results highlight that the performance of the commonly adopted ABL similarity schemes (e.g. flux-gradient relationships and parameterizations for the stable ABL height) depends upon the ABL state, determined mainly by the wind speed and the shape of the profiles of second order moments (the two being related) . For neutral or

  10. Turbulence feature modifications from high to low wind conditions: results from the CCT observations at Ny-Ålesund, Svalbard.

    Science.gov (United States)

    Schiavon, Mario; Mazzola, Mauro; Tampieri, Francesco; Pietro Viola, Angelo; Choi, Taejin

    2017-04-01

    The turbulence features in the quasi neutral surface layer are investigated as the intensity of the wind decreases, i.e. as the forcing due to the shear decreases. In this aim, a 5-year (2012-2016) set of observations of meteorological and micro-meteorological parameters acquired on the Climate Change Tower (CCT) in Ny-Ålesund, Svalbard Islands, is used. The 34-m high tower, operated by the Italian National Council of Research (CNR) is equipped with four slow response wind and temperature probes and three fast response sonic anemometers and is located on heterogeneous terrain. One of the fast sensors was installed by KOPRI since 2012. The observations are averaged over 10 and 30 minutes intervals. The analysis addresses the share of the mean turbulent kinetic energy (TKE) among the along-wind, cross-wind and vertical velocity variances (respectively u2 >, , ), with attention to the parameterizations of the boundary layer commonly used in NWP models: the classical Mellor-Yamada (1982) scheme with the return-to-isotropy term by Rotta(1951) and its modifications, and the recent approach by Zilitinkevich and coworkers (2013). The results show that the share of TKE among the vertical and the total horizontal variance u2 > + is weakly dependent on the wind velocity while the share of the total horizontal variance between the along-wind and cross-wind components depends on wind speed. At high velocity (and large wind shear) a clear anisotropy , with u2 >≈ 2 , is observed, quite consistent with literature (Tampieri, 2017, pag. 69). As the velocity decreases, the ratio u2 > /(u2 > + ) displays a wide flat distribution between 0.2 and 0.8 with median values corresponding approximately to horizontal isotropy: u2 >≈. These features can be parameterized using suitable coefficients, function of the wind intensity in the equations for the TKE share, capturing the average behaviour of the flow. A further investigation based on estimates of the relative importance of the high

  11. Effect of rutile TiO2 on the photocatalytic performance of g-C3N4/brookite-TiO2-xNy photocatalyst for NO decomposition

    Science.gov (United States)

    Li, Huihui; Wu, Xiaoyong; Yin, Shu; Katsumata, Kenichi; Wang, Yuhua

    2017-01-01

    Novel g-C3N4/rutile-brookite TiO2-xNy composite photocatalysts were fabricated through a facile solvothermal approach. The effect of rutile phase TiO2 with brookite TiO2 and g-C3N4 on the photocatalytic activity of g-C3N4/nitrogen-doped TiO2 composite was studied. The photocatalytic performance of the photocatalyst was evaluated by measuring the degradation of NO gas under visible and UV light irradiation. It is suggested that g-C3N4/rutile-brookite TiO2-xNy forms a Z-scheme photocatalytic system, which shows improvement on the photocatalytic activity than that of g-C3N4/single brookite TiO2-xNy. By importing rutile phase TiO2-xNy, the photogenerated electrons can efficiently transfer from rutile TiO2 to g-C3N4, which results in the separation of electron and hole pairs, enhancing the photocatalytic ability. However, single brookite TiO2-xNy can not remove the photogenerated electrons efficiently and the photocatalytic performances of composites decrease with g-C3N4 amount increase.

  12. BIVALVES AND BRACHIOPODS NEAR THE PERMIAN-TRIASSIC BOUNDARY FROM THE BÜKK MOUNTAINS (BÁLVÁNY-NORTH SECTION, NORTHERN HUNGARY

    Directory of Open Access Journals (Sweden)

    RENATO POSENATO

    2005-07-01

    Full Text Available In the Bükk Mountains the Upper Permian is represented by the Nagyvisnyó Limestone, which contains very rich marine assemblages. It is overlain by the Gerennavár Limestone (uppermost Permian-Lower Triassic which records the effects of the Permo-Triassic mass extinction with a dramatic decrease in diversity and abundance of fossils. The basal Gerennavár Limestone is represented by a clayey marl unit (basal beds deposited in a quiet, low-energy marine environment below the storm wave-base, whose maximum thickness, about one meter, is recorded in the Bálvány-North section. From this locality a relatively diversified and abundant marine benthonic assemblage has been collected, and is here described. Bivalves are represented by: Bakevellia cf. ceratophaga (Schlotheim, ? Pterinopectinidae gen. et sp. indet., Eumorphotis lorigae sp. n., the most abundant species, Entolium piriformis (Liu and Pernopecten latangulatus Yin. Brachiopods are less frequent, and the following four species have been identified: Spinomarginifera sp., Orthothetina ladina (Stache, Ombonia tirolensis (Stache and Orbicoelia tschernyschewi (Likharew. An exact age of this fauna, based on conodonts, is not yet available, but the strong affinities with those of the lower Tesero Member (Dolomites and the Lower Kathwai Member (Pakistan suggest a latest Permian age (? Hindeodus praeparvus Zone. If so, the Bálvány-North section becomes one of the few in the world which records the last bioevents of the Palaeozoic.

  13. Recombination of strain O segments to HCpro-encoding sequence of strain N of Potato virus Y modulates necrosis induced in tobacco and in potatoes carrying resistance genes Ny or Nc.

    Science.gov (United States)

    Tian, Yan-Ping; Valkonen, Jari P T

    2015-09-01

    Hypersensitive resistance (HR) to strains O and C of Potato virus Y (PVY, genus Potyvirus) is conferred by potato genes Ny(tbr) and Nc(tbr), respectively; however, PVY N strains overcome these resistance genes. The viral helper component proteinases (HCpro, 456 amino acids) from PVY(N) and PVY(O) are distinguished by an eight-amino-acid signature sequence, causing HCpro to fold into alternative conformations. Substitution of only two residues (K269R and R270K) of the eight-amino-acid signature in PVY(N) HCpro was needed to convert the three-dimensional (3D) model of PVY(N) HCpro to a PVY(O) -like conformation and render PVY(N) avirulent in the presence of Ny(tbr), whereas four amino acid substitutions were necessary to change PVY(O) HCpro to a PVY(N) -like conformation. Hence, the HCpro conformation rather than other features ascribed to the sequence were essential for recognition by Ny(tbr). The 3D model of PVY(C) HCpro closely resembled PVY(O), but differed from PVY(N) HCpro. HCpro of all strains was structurally similar to β-catenin. Sixteen PVY(N) 605-based chimeras were inoculated to potato cv. Pentland Crown (Ny(tbr)), King Edward (Nc(tbr)) and Pentland Ivory (Ny(tbr)/Nc(tbr)). Eleven chimeras induced necrotic local lesions and caused no systemic infection, and thus differed from both parental viruses that infected King Edward systemically, and from PVY(N) 605 that infected Pentland Crown and Pentland Ivory systemically. These 11 chimeras triggered both Ny(tbr) and Nc(tbr) and, in addition, six induced veinal necrosis in tobacco. Further, specific amino acid residues were found to have an additive impact on necrosis. These results shed new light on the causes of PVY-related necrotic symptoms in potato. © 2014 BSPP AND JOHN WILEY & SONS LTD.

  14. Production of NY-ESO-1 peptide/DRB1*08:03 tetramers and ex vivo detection of CD4 T-cell responses in vaccinated cancer patients.

    Science.gov (United States)

    Mizote, Yu; Uenaka, Akiko; Isobe, Midori; Wada, Hisashi; Kakimi, Kazuhiro; Saika, Takashi; Kita, Shoichi; Koide, Yukari; Oka, Mikio; Nakayama, Eiichi

    2014-02-12

    We established CD4 T-cell clones, Mz-1B7, and Ue-21, which recognized the NY-ESO-1 121-138 peptide from peripheral blood mononuclear cells (PBMCs) of an esophageal cancer patient, E-2, immunized with an NY-ESO-1 protein and determined the NY-ESO-1 minimal epitopes. Minimal peptides recognized by Mz-1B7 and Ue-21 were NY-ESO-1 125-134 and 124-134, respectively, both in restriction to DRB1*08:03. Using a longer peptide, 122-135, and five other related peptides, including either of the minimal epitopes recognized by the CD4 T-cell clones, we investigated the free peptide/DR recognition on autologous EBV-B cells as APC and peptide/DR tetramer binding. The results showed a discrepancy between them. The tetramers with several peptides recognized by either Mz-1B7 or the Ue-21 CD4 T-cell clone did not bind to the respective clone. On the other hand, unexpected binding of the tetramer with the peptide not recognized by CD4 T-cells was observed. The clone Mz-1B7 did not recognize the free peptide 122-135 on APC, but the peptide 122-135/DRB1*08:03 tetramer bound to the TCR on those cells. The failure of tetramer production and the unexpected tetramer binding could be due to a subtly modified structure of the peptide/DR tetramer from the structure of the free peptide/DR molecule. We also demonstrated that the NY-ESO-1 123-135/DRB1*08:03 tetramer detected ex vivo CD4 T-cell responses in PBMCs from patients after NY-ESO-1 vaccination in immunomonitoring. Copyright © 2013 Elsevier Ltd. All rights reserved.

  15. Partnership to Enhance Diversity in Marine Geosciences: Holocene Climate and Anthorpogenic Changes from Long Island Sound, NY

    Science.gov (United States)

    McHugh, C. M.; Cormier, M.; Marchese, P.; Zheng, Y.; Kohfeld, K.

    2006-12-01

    assistance to attend college; and 3) through our regular teaching schedule for non-geoscience majors. The PIs work at four different institutions from NY metropolitan area: 1) Queens College (QC) from the City University of New York (CUNY.); 2) Queensborough Community College (CUNY), a minority serving college; 3) Lehman College (CUNY), a minority serving college; and 4) Lamont-Doherty Earth Observatory (LDEO), a world-class earth science research institution. External financial support for the program has been secured through the City University of New York Graduate Center through a MAGNET fellowship, Minorities Striving and Pursuing Higher Degrees of Success in Earth Systems Science Professional Development Program 2006, through two industry grants (Entergy Co. and NRG Energy), and in the future, through the availability at competitive rates of local research vessels for day- trips. We anticipate that the program will further gain momentum through partnerships with other City University of New York senior and community colleges, and thanks to word of mouth from those students who participated in the program.

  16. 关于丢番图方程(195n)x+(28n)y=(197n)z%On the Diophantine equation (195n)x+(28n)y=(197n)z

    Institute of Scientific and Technical Information of China (English)

    凌灯荣; 翁建欣

    2013-01-01

    In this paper, using the properties of congruences and the order of elements, we show that for any positive integer n, the Diophantine equation (195n)x+(28n)y =(197n)z has no solution other than (x, y, z)=(2, 2, 2) in positive integers.%  运用同余及元素阶的性质,证明了对任意的正整数n,丢番图方程(195n)x+(28n)y=(197n)z仅有正整数解(x, y, z)=(2,2,2)。

  17. Temporal Variations of the Atmospheric CO2 Concentration and Its Carbon Isotope Ratio at Ny-Ålesund, Svalbard and Estimation of Global Carbon Budget

    Science.gov (United States)

    Goto, D.; Morimoto, S.; Ishidoya, S.; Aoki, S.; Nakazawa, T.

    2016-12-01

    Long-term measurements of the atmospheric CO2 concentration and its carbon isotope ratio (δ13C) are useful for partitioning anthropogenic CO2 into the terrestrial biosphere and the ocean, if the carbon isotopic disequilibrium flux (so-called isoflux), combining terrestrial and oceanic contributions, is quantified. For a better understanding of the global carbon cycle, we have carried out the systematic observation of the atmospheric CO2 concentration and δ13C at Ny-Ålesund, Svalbard (78.93°N, 11.83°E) since 1991. Air samples were collected into stainless-steel flasks at the Japanese observatory in Ny-Ålesund, once a week and sent to NIPR every two months. CO2 concentrations of the air samples were determined by using a NDIR analyzer, and CO2 samples extracted cryogenically from the remaining air in the flasks were analyzed for δ13C using a mass spectrometer. Analytical precisions for CO2 and δ13C were 0.01 ppm and 0.02 ‰, respectively. The CO2 concentration shows a clear seasonal cycle with peak-to-peak amplitude of about 17 ppm, which reaches a maximum in late April to early May and a minimum in late August, superimposed on a secular increase with an average rate of 2.0 ppm/yr for the period of 1996-2013. On the other hand, the δ13C decreases secularly at an average rate of -0.018 ‰/yr, and varies seasonally in opposite phase with the CO2 concentration. By analyzing the CO2 concentration and δ13C using the isoflux calculated with a box-diffusion model, the terrestrial biospheric and oceanic CO2 sinks are estimated to be 1.5 ± 0.3 and 2.4 ± 0.4 GtC/yr, respectively, for the 13-year period (2001-2013). On the other hand, the secular trends of the atmospheric δ(O2/N2) and CO2 concentration at Ny-Ålesund (Ishidoya et al., 2012) yield the respective sink values of 1.7 ± 0.8 and 2.2 ± 0.7 GtC/yr for the same period. The estimates from the two methods are in good agreement with each other.

  18. Návrh změny organizační kultury v Domově seniorů Břeclav

    OpenAIRE

    Bukvová, Alena

    2009-01-01

    Bakalářská práce se zabývá tématem organizační kultury a jejím vlivem na efektivnost Domova seniorů Břeclav. Cílem bakalářské práce bylo analyzovat obsah organizační kultury vybrané organizace a posoudit její obsah ve vztahu k efektivnosti organizace. Na základě výsledků provedeného výzkumu byly pak navrženy takové změny obsahu organizační kultury, které by ji přiblížily směrem ke kultuře žádoucí. The bachelor thesis deals with organizational culture and its effect on efficiency of the Ret...

  19. En dag med vin och historia : Utveckling av en ny produkt för Finlands Skärgårdsbokning

    OpenAIRE

    Salminen, Helen

    2013-01-01

    Dagens resenärer är allt mera intresserade av lokal producerad vin och mat. Under 1990-talet växte enoturismen fram som en nisch och efter alkohollagförändringen 1995 kunde enoturismen också slå rot i Finland. Idag finns det 25 vingårdar runtom i Finland, majoriteten av dessa ligger i södra Finland. Syftet med detta examensarbete är att genom tjänsteutveckling skapa en ny produkt för Finlands Skärgårdsbokning där jag sammanbinder Åbolands Skärgård och enoturismen. För att kunna utveckla denna...

  20. A pilot study of peripheral blood BDCA-1 (CD1c) positive dendritic cells pulsed with NY-ESO-1 ISCOMATRIX™ adjuvant.

    Science.gov (United States)

    Davis, Ian D; Quirk, Juliet; Morris, Leone; Seddon, Lauren; Tai, Tsin Yee; Whitty, Genevieve; Cavicchiolo, Tina; Ebert, Lisa; Jackson, Heather; Browning, Judy; MacGregor, Duncan; Wittke, Frederick; Winkels, Gregor; Alex, Regina; Miloradovic, Lena; Maraskovsky, Eugene; Chen, Weisan; Cebon, Jonathan

    2017-03-01

    Pilot clinical trial of NY-ESO-1 (ESO) protein in ISCOMATRIX™ adjuvant pulsed onto peripheral blood dendritic cells (PBDC), to ascertain feasibility, evaluate toxicity and assess induction of ESO-specific immune responses. Eligible participants had resected cancers expressing ESO or LAGE-1 and were at high risk of relapse. PBDC were produced using CliniMACS(®)plus, with initial depletion of CD1c(+) B cells followed by positive selection of CD1c(+) PBDC. Patients received three intradermal vaccinations of ESO/IMX-pulsed PBDC at 4-week intervals. The process was feasible and safe. No vaccine-induced immune responses were detected. Assays of immunomodulatory cells did not correlate with outcomes. One patient had a long lasting complete remission. This method was feasible and safe but was minimally immunogenic.

  1. Major Histocompatibility Complex (MHC) Class I Processing of the NY-ESO-1 Antigen Is Regulated by Rpn10 and Rpn13 Proteins and Immunoproteasomes following Non-lysine Ubiquitination.

    Science.gov (United States)

    Golnik, Richard; Lehmann, Andrea; Kloetzel, Peter-Michael; Ebstein, Frédéric

    2016-04-15

    The supply of MHC class I-restricted peptides is primarily ensured by the degradation of intracellular proteins via the ubiquitin-proteasome system. Depending on the target and the enzymes involved, ubiquitination is a process that may dramatically vary in terms of linkages, length, and attachment sites. Here we identified the unique lysine residue at position 124 of the NY-ESO-1 cancer/testis antigen as the acceptor site for the formation of canonical Lys-48-linkages. Interestingly, a lysine-less form of NY-ESO-1 was as efficient as its wild-type counterpart in supplying the HLA-A*0201-restricted NY-ESO-1157-165 antigenic peptide. In fact, we show that the regulation of NY-ESO-1 processing by the ubiquitin receptors Rpn10 and Rpn13 as a well as by the standard and immunoproteasome is governed by non-canonical ubiquitination on non-lysine sites. In summary, our data underscore the significance of atypical ubiquitination in the modulation of MHC class I antigen processing. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  2. Major Histocompatibility Complex (MHC) Class I Processing of the NY-ESO-1 Antigen Is Regulated by Rpn10 and Rpn13 Proteins and Immunoproteasomes following Non-lysine Ubiquitination*

    Science.gov (United States)

    Golnik, Richard; Lehmann, Andrea; Kloetzel, Peter-Michael; Ebstein, Frédéric

    2016-01-01

    The supply of MHC class I-restricted peptides is primarily ensured by the degradation of intracellular proteins via the ubiquitin-proteasome system. Depending on the target and the enzymes involved, ubiquitination is a process that may dramatically vary in terms of linkages, length, and attachment sites. Here we identified the unique lysine residue at position 124 of the NY-ESO-1 cancer/testis antigen as the acceptor site for the formation of canonical Lys-48-linkages. Interestingly, a lysine-less form of NY-ESO-1 was as efficient as its wild-type counterpart in supplying the HLA-A*0201-restricted NY-ESO-1157–165 antigenic peptide. In fact, we show that the regulation of NY-ESO-1 processing by the ubiquitin receptors Rpn10 and Rpn13 as a well as by the standard and immunoproteasome is governed by non-canonical ubiquitination on non-lysine sites. In summary, our data underscore the significance of atypical ubiquitination in the modulation of MHC class I antigen processing. PMID:26903513

  3. First-in-Human Treatment With a Dendritic Cell-targeting Lentiviral Vector-expressing NY-ESO-1, LV305, Induces Deep, Durable Response in Refractory Metastatic Synovial Sarcoma Patient.

    Science.gov (United States)

    Pollack, Seth M; Lu, Hailing; Gnjatic, Sacha; Somaiah, Neeta; O'Malley, Ryan B; Jones, Robin L; Hsu, Frank J; Ter Meulen, Jan

    2017-10-01

    Effective induction of antitumor T cells is a pivotal goal of cancer immunotherapy. To this end, lentiviral vectors (LV) are uniquely poised to directly prime CD8 T-cell responses via transduction of dendritic cells in vivo and have shown promise as active cancer therapeutics in preclinical tumor models. However, until now, significant barriers related to production and regulation have prevented their widespread use in the clinic. We developed LV305, a dendritic cell-targeting, integration-deficient, replication incompetent LV from the ZVex platform, encoding the full-length cancer-testis antigen NY-ESO-1. LV305 is currently being evaluated in phase 1 and 2 trials in metastatic recurrent cancer patients with NY-ESO-1 positive solid tumors as a single agent and in combination with anti-PD-L1. Here we report on the first patient treated with LV305, a young woman with metastatic, recurrent, therapy-refractive NY-ESO-1 synovial sarcoma. The patient developed a robust NY-ESO-1-specific CD4 and CD8 T-cell response after 3 intradermal injections with LV305, and subsequently over 85% disease regression that is continuing for >2.5 years posttherapy. No adverse events >grade 2 occurred. This case demonstrates that LV305 can be safely administered and has the potential to induce a significant clinical benefit and immunologic response in a patient with advanced stage cancer.

  4. 区域艺术发展势头强劲-ACAF NY 2008执行总监Cristal Kim访谈%Strong momentum of regional art development-Special interview of Cristal Kim,executive supervisor of ACAF NY 2008

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ ACAF是一个中型的艺术博览会,注重与本地对亚洲当代文化和艺术有兴趣的画廊、机构和企业的合作,同时致力于在纽约提升亚洲的当代文化和艺术.在ACAF2008即将举办之际,对ACAF NY 2008执行总监Cristal Kim进行了专访.

  5. NY-ESO-1 TCR single edited stem and central memory T cells to treat multiple myeloma without graft-versus-host disease.

    Science.gov (United States)

    Mastaglio, Sara; Genovese, Pietro; Magnani, Zulma; Ruggiero, Eliana; Landoni, Elisa; Camisa, Barbara; Schiroli, Giulia; Provasi, Elena; Lombardo, Angelo; Reik, Andreas; Cieri, Nicoletta; Rocchi, Martina; Oliveira, Giacomo; Escobar, Giulia; Casucci, Monica; Gentner, Bernhard; Spinelli, Antonello; Mondino, Anna; Bondanza, Attilio; Vago, Luca; Ponzoni, Maurilio; Ciceri, Fabio; Holmes, Michael C; Naldini, Luigi; Bonini, Chiara

    2017-08-03

    Transfer of T-cell receptors (TCRs) specific for tumor-associated antigens is a promising approach for cancer immunotherapy. We developed the TCR gene editing technology that is based on the knockout of the endogenous TCR α and β genes, followed by the introduction of tumor-specific TCR genes, and that proved safer and more effective than conventional TCR gene transfer. Although successful, complete editing requires extensive cell manipulation and 4 transduction procedures. Here we propose a novel and clinically feasible TCR "single editing" (SE) approach, based on the disruption of the endogenous TCR α chain only, followed by the transfer of genes encoding for a tumor-specific TCR. We validated SE with the clinical grade HLA-A2 restricted NY-ESO-1157-165-specific TCR. SE allowed the rapid production of high numbers of tumor-specific T cells, with optimal TCR expression and preferential stem memory and central memory phenotype. Similarly to unedited T cells redirected by TCR gene transfer (TCR transferred [TR]), SE T cells efficiently killed NY-ESO-1(pos) targets; however, although TR cells proved highly alloreactive, SE cells showed a favorable safety profile. Accordingly, when infused in NSG mice previously engrafted with myeloma, SE cells mediated tumor rejection without inducing xenogeneic graft-versus-host disease, thus resulting in significantly higher survival than that observed in mice treated with TR cells. Overall, single TCR gene editing represents a clinically feasible approach that is able to increase the safety and efficacy of cancer adoptive immunotherapy. © 2017 by The American Society of Hematology.

  6. Case-Control Study: Smoking History Affects the Production of Tumor Antigen-Specific Antibodies NY-ESO-1 in Patients with Lung Cancer in Comparison with Cancer Disease-Free Group.

    Science.gov (United States)

    Myšíková, Dagmar; Adkins, Irena; Hradilová, Nad'a; Palata, Ondřej; Šimonek, Jan; Pozniak, Jiří; Kolařík, Jan; Skallová-Fialová, Anna; Špíšek, Radek; Lischke, Robert

    2017-02-01

    Lung cancer is the leading cause of cancer mortality worldwide; therefore, understanding the biological or clinical role of tumor-associated antigens and autoantibodies is of eminent interest for designing antitumor immunotherapeutic strategies. Here we prospectively analyzed the serum frequencies of New York esophageal squamous cell carcinoma 1 (NY-ESO-1), human epidermal growth factor 2/neu, and melanoma-associated antigen A4 (MAGE-A4) antibodies and expression of the corresponding antigens in tumors of 121 patients with NSCLC undergoing an operation without prior neoadjuvant chemotherapy and compared them with those in 57 control age-matched patients with no history of a malignant disease. We found that only antibodies specific for NY-ESO-1 (19.8% [n = 24 of 121]) were significantly increased in the group of patients with NSCLC compared with in the controls. NY-ESO-1 seropositivity was significantly positively associated with an active smoking history in patients with NSCLC but not in smokers from the control group. In tumors, the frequency of NY-ESO-1 mRNA expression was 6.3% (in four of 64 patients), the frequency of human epidermal growth factor 2/neu (HER 2/neu) expression was 11.9% (five of 42), and the frequency of MAGE-A4 expression was 35.1% (20 of 57). MAGE-A4 expression in tumors correlated with smoking status and male sex in patients with NSCLC. Patients with squamous cell carcinoma displayed higher expression of NY-ESO-1 and MAGE-A4 in tumors than did patients with adenocarcinoma. On the other hand, 94.7% of nonsmoking patients in our study had adenocarcinoma (of whom 73.7% were women). These results confirm the reported high immunogenicity of NY-ESO-1 and suggest that a smoking-induced chronic inflammatory state might potentiate the development of NY-ESO-1-specific immune responses. Moreover, smoking might contribute to the expression of other cancer/testis antigens such as MAGE-A4 at early stages of NSCLC development. Copyright © 2016

  7. Circulating CD4+ T cells that produce IL4 or IL17 when stimulated by melan-A but not by NY-ESO-1 have negative impacts on survival of patients with stage IV melanoma.

    Science.gov (United States)

    Zelba, Henning; Weide, Benjamin; Martens, Alexander; Derhovanessian, Evelyna; Bailur, Jithendra Kini; Kyzirakos, Christina; Pflugfelder, Annette; Eigentler, Thomas K; Di Giacomo, Anna Maria; Maio, Michele; Aarntzen, Erik H J G; de Vries, Jolanda; Sucker, Antje; Schadendorf, Dirk; Büttner, Petra; Garbe, Claus; Pawelec, Graham

    2014-08-15

    We initially observed that the presence of circulating NY-ESO-1- and/or Melan-A-specific T cells in patients with stage IV melanoma was significantly associated with prolonged survival. Here, we report the ways in which the phenotypes and functions of these T cells differentially affect survival in patients preselected for NY-ESO-1 and/or Melan-A reactivity. We assayed functional antigen-reactive T cells recognizing NY-ESO-1 and/or Melan-A after in vitro stimulation using overlapping peptide pools. After restimulation, we assayed six cytokines simultaneously by intracellular cytokine staining. This allowed us to analyze the functional antigen response of both CD4(+) and CD8(+) T cells at the single-cell level. We observed that NY-ESO-1 stimulated mainly CD4(+) T cells, whereas Melan-A more often stimulated CD8(+) T cells. NY-ESO-1 reactivity was not associated with an additional impact on survival, whether CD4(+) T cells, CD8(+) T cells, or both types of T cells were responding. In contrast, recognition of Melan-A by CD4(+) T cells was associated with reduced survival in our cohort of patients preselected for NY-ESO-1 and/or Melan-A reactivity (that is, in patients with exceptionally long survival). We further observed a negative effect on survival in patients with CD4(+) T cells producing IL4 and IL17 upon Melan-A stimulation. Their prognosis was comparable to patients without any Melan-A reactivity. The nature and prognostic impact of specific T-cell responses is different according to targeted antigen. Independent from phenotype and functional aspects, NY-ESO-1 reactivity is associated with good prognosis. In terms of Melan-A, antigen-specific CD8(+) but not CD4(+) responses are associated with prolonged survival. Clin Cancer Res; 20(16); 4390-9. ©2014 AACR. ©2014 American Association for Cancer Research.

  8. Detection and Tracking of NY-ESO-1-Specific CD8+ T Cells by High-Throughput T Cell Receptor β (TCRB) Gene Rearrangements Sequencing in a Peptide-Vaccinated Patient.

    Science.gov (United States)

    Miyai, Manami; Eikawa, Shingo; Hosoi, Akihiro; Iino, Tamaki; Matsushita, Hirokazu; Isobe, Midori; Uenaka, Akiko; Udono, Heiichiro; Nakajima, Jun; Nakayama, Eiichi; Kakimi, Kazuhiro

    2015-01-01

    Comprehensive immunological evaluation is crucial for monitoring patients undergoing antigen-specific cancer immunotherapy. The identification and quantification of T cell responses is most important for the further development of such therapies. Using well-characterized clinical samples from a high responder patient (TK-f01) in an NY-ESO-1f peptide vaccine study, we performed high-throughput T cell receptor β-chain (TCRB) gene next generation sequencing (NGS) to monitor the frequency of NY-ESO-1-specific CD8+ T cells. We compared these results with those of conventional immunological assays, such as IFN-γ capture, tetramer binding and limiting dilution clonality assays. We sequenced human TCRB complementarity-determining region 3 (CDR3) rearrangements of two NY-ESO-1f-specific CD8+ T cell clones, 6-8L and 2F6, as well as PBMCs over the course of peptide vaccination. Clone 6-8L possessed the TCRB CDR3 gene TCRBV11-03*01 and BJ02-01*01 with amino acid sequence CASSLRGNEQFF, whereas 2F6 possessed TCRBV05-08*01 and BJ02-04*01 (CASSLVGTNIQYF). Using these two sequences as models, we evaluated the frequency of NY-ESO-1-specific CD8+ T cells in PBMCs ex vivo. The 6-8L CDR3 sequence was the second most frequent in PBMC and was present at high frequency (0.7133%) even prior to vaccination, and sustained over the course of vaccination. Despite a marked expansion of NY-ESO-1-specific CD8+ T cells detected from the first through 6th vaccination by tetramer staining and IFN-γ capture assays, as evaluated by CDR3 sequencing the frequency did not increase with increasing rounds of peptide vaccination. By clonal analysis using 12 day in vitro stimulation, the frequency of B*52:01-restricted NY-ESO-1f peptide-specific CD8+ T cells in PBMCs was estimated as only 0.0023%, far below the 0.7133% by NGS sequencing. Thus, assays requiring in vitro stimulation might be underestimating the frequency of clones with lower proliferation potential. High-throughput TCRB sequencing using NGS

  9. Detection and Tracking of NY-ESO-1-Specific CD8+ T Cells by High-Throughput T Cell Receptor β (TCRB Gene Rearrangements Sequencing in a Peptide-Vaccinated Patient.

    Directory of Open Access Journals (Sweden)

    Manami Miyai

    Full Text Available Comprehensive immunological evaluation is crucial for monitoring patients undergoing antigen-specific cancer immunotherapy. The identification and quantification of T cell responses is most important for the further development of such therapies. Using well-characterized clinical samples from a high responder patient (TK-f01 in an NY-ESO-1f peptide vaccine study, we performed high-throughput T cell receptor β-chain (TCRB gene next generation sequencing (NGS to monitor the frequency of NY-ESO-1-specific CD8+ T cells. We compared these results with those of conventional immunological assays, such as IFN-γ capture, tetramer binding and limiting dilution clonality assays. We sequenced human TCRB complementarity-determining region 3 (CDR3 rearrangements of two NY-ESO-1f-specific CD8+ T cell clones, 6-8L and 2F6, as well as PBMCs over the course of peptide vaccination. Clone 6-8L possessed the TCRB CDR3 gene TCRBV11-03*01 and BJ02-01*01 with amino acid sequence CASSLRGNEQFF, whereas 2F6 possessed TCRBV05-08*01 and BJ02-04*01 (CASSLVGTNIQYF. Using these two sequences as models, we evaluated the frequency of NY-ESO-1-specific CD8+ T cells in PBMCs ex vivo. The 6-8L CDR3 sequence was the second most frequent in PBMC and was present at high frequency (0.7133% even prior to vaccination, and sustained over the course of vaccination. Despite a marked expansion of NY-ESO-1-specific CD8+ T cells detected from the first through 6th vaccination by tetramer staining and IFN-γ capture assays, as evaluated by CDR3 sequencing the frequency did not increase with increasing rounds of peptide vaccination. By clonal analysis using 12 day in vitro stimulation, the frequency of B*52:01-restricted NY-ESO-1f peptide-specific CD8+ T cells in PBMCs was estimated as only 0.0023%, far below the 0.7133% by NGS sequencing. Thus, assays requiring in vitro stimulation might be underestimating the frequency of clones with lower proliferation potential. High-throughput TCRB

  10. Aerosol absorption coefficient and Equivalent Black Carbon by parallel operation of AE31 and AE33 aethalometers at the Zeppelin station, Ny Ålesund, Svalbard

    Science.gov (United States)

    Eleftheriadis, Konstantinos; Kalogridis, Athina-Cerise; Vratolis, Sterios; Fiebig, Markus

    2016-04-01

    Light absorbing carbon in atmospheric aerosol plays a critical role in radiative forcing and climate change. Despite the long term measurements across the Arctic, comparing data obtained by a variety of methods across stations requires caution. A method for extracting the aerosol absorption coefficient from data obtained over the decades by filter based instrument is still under development. An IASOA Aerosol working group has been initiated to address this and other cross-site aerosol comparison opportunities. Continuous ambient measurements of EBC/light attenuation by means of a Magee Sci. AE-31 aethalometer operating at the Zeppelinfjellet station (474 m asl; 78°54'N, 11°53'E), Ny Ålesund, Svalbard, have been available since 2001 (Eleftheriadis et al, 2009), while a new aethalometer model (AE33, Drinovec et al, 2014) has been installed to operate in parallel from the same inlet since June 2015. Measurements are recorded by a Labview routine collecting all available parameters reported by the two instrument via RS232 protocol. Data are reported at 1 and 10 minute intervals as averages for EBC (μg m-3) and aerosol absorption coefficients (Mm-1) by means of routine designed to report Near Real Time NRT data at the EBAS WDCA database (ebas.nilu.no) Results for the first 6 month period are reported here in an attempt to evaluate comparative performance of the two instruments in terms of their response with respect to the variable aerosol load of light absorbing carbon during the warm and cold seasons found in the high arctic. The application of available conversion schemes for obtaining the absorption coefficient by the two instruments is found to demonstrate a marked difference in their output. During clean periods of low aerosol load (EBC agreement with regression slope for the 880 nm signal between the two at ~ 0.9 compared to a slope at ~ 0.6 during the period of higher absorbing carbon loads (400Arctic: Measurements at Zeppelin station, Ny-Ålesund, Svalbard

  11. 75 FR 53731 - Culturally Significant Objects Imported for Exhibition Determinations: “Sheila Hicks: 50 Years”

    Science.gov (United States)

    2010-09-01

    ... on or about November 5, 2010, until on or about February 27, 2011; at the Institute of Contemporary Art, University of Pennsylvania, Philadelphia, PA, from on or about March 25, 2011, until on or...

  12. The Pre-Anschluss Vienna School of Medicine--The surgeons: Ignaz Semmelweis (1818-1865), Theodor Billroth (1829-1894) and Robert Bárány (1876-1936).

    Science.gov (United States)

    Shaw, Lily B Z L; Shaw, Robert A

    2016-02-01

    A brief history of the Vienna School of Medicine is sketched out from its founding in the mid-18th century by Gerard van Swieten until the Anschluss in March 1938. The pioneering work of Ignaz Semmelweis on the causes and the prevention of puerperal fever is discussed. This is followed by ground-breaking innovations, particularly in abdominal surgery, by Theodor Billroth and by Robert Bárány's Nobel Prize winning work inter alia on defining the pathology and physiology of the human vestibular apparatus. The lives and work of these three outstanding medical practitioners are described, together with their successes and failures. Only Billroth's achievements were appreciated in Vienna during their lifetimes. Semmelweis' work was belittled during his lifetime and he died obscurely in a mental institution. Bárány had to immigrate to Sweden to achieve recognition.

  13. Sentikių Bažnyčia Lietuvoje 1918 - 1940 metais: teisinė padėtis, religinė organizacija, visuomeninė politinė sklaida

    OpenAIRE

    2009-01-01

    Sentikiai prieglobstį Lietuvoje rado jau XVII a., netrukus po to, kai po Rusijos patriarcho Nikono įvykdytų Bažnyčios reformų, daliai tikinčiųjų atsisakius pripažinti ir atsirado sentikystė. Lietuvos sentikiai priklauso antrai rusų migracijos į Lietuvą bangai. Šiandien Lietuvoje Sentikių religinė bendruomenė turi tradicinės religinės bendruomenės statusą. Darbo temos Sentikių Bažnyčia Lietuvoje 1918 -1940 metais: teisinė padėtis, religinė organizacija, visuomeninė politinė sklaida tyrimo obje...

  14. Diversity and Distribution of Aquatic Fungal Communities in the Ny-Ålesund Region, Svalbard (High Arctic): Aquatic Fungi in the Arctic.

    Science.gov (United States)

    Zhang, Tao; Wang, Neng-Fei; Zhang, Yu-Qin; Liu, Hong-Yu; Yu, Li-Yan

    2016-04-01

    We assessed the diversity and distribution of fungi in 13 water samples collected from four aquatic environments (stream, pond, melting ice water, and estuary) in the Ny-Ålesund Region, Svalbard (High Arctic) using 454 pyrosequencing with fungi-specific primers targeting the internal transcribed spacer (ITS) region of the ribosomal rRNA gene. Aquatic fungal communities in this region showed high diversity, with a total of 43,061 reads belonging to 641 operational taxonomic units (OTUs) being found. Of these OTUs, 200 belonged to Ascomycota, 196 to Chytridiomycota, 120 to Basidiomycota, 13 to Glomeromycota, and 10 to early diverging fungal lineages (traditional Zygomycota), whereas 102 belonged to unknown fungi. The major orders were Helotiales, Eurotiales, and Pleosporales in Ascomycota; Chytridiales and Rhizophydiales in Chytridiomycota; and Leucosporidiales and Sporidiobolales in Basidiomycota. The common fungal genera Penicillium, Rhodotorula, Epicoccum, Glaciozyma, Holtermanniella, Betamyces, and Phoma were identified. Interestingly, the four aquatic environments in this region harbored different aquatic fungal communities. Salinity, conductivity, and temperature were important factors in determining the aquatic fungal diversity and community composition. The results suggest the presence of diverse fungal communities and a considerable number of potentially novel fungal species in Arctic aquatic environments, which can provide reliable data for studying the ecological and evolutionary responses of fungi to climate change in the Arctic ecosystem.

  15. Breakdown field enhancement of Si-based MOS capacitor by post-deposition annealing of the reactive sputtered ZrOxNy gate oxide

    Science.gov (United States)

    Chew, Chun Chet; Goh, Kian Heng; Gorji, Mohammad Saleh; Tan, Chee Ghuan; Ramesh, S.; Wong, Yew Hoong

    2016-02-01

    Zirconium oxynitride (ZrOxNy) thin films were deposited on silicon (100) substrates by radio frequency-reactive magnetron sputtering in an argon-oxygen-nitrogen atmosphere. Post-deposition annealing (PDA) process was performed in argon ambient at various annealing temperatures (500, 600, 700 and 800 °C) for 15 min. Metal-oxide-semiconductor capacitors were then fabricated with aluminum as the gate electrode. The effects of PDA process on the thin film's structural and electrical properties of the samples were investigated. The structural properties of the deposited films have been evaluated by atomic force microscopy, Fourier transform infrared spectroscopy and Raman spectroscopy. On the other hand, the electrical characterization of the film was conducted by current-voltage analysis. The Raman results revealed that (600-800 °C) annealed samples comprised of crystalline multiphase films (t-ZrO2, fcc-ZrN and bcc γ-Zr2ON2). Interfacial layer consisted of Zr-Si-O, Si-O-N and Si-O phase was formed for all investigated samples, and interfacial layer growth was suppressed when annealed at lower temperatures (500 °C). Electrical result revealed that the sample annealed at a relatively low temperature of 500 °C has demonstrated the highest breakdown field which was attributed to the low surface roughness, the low interface trap and the highly amorphous multiphase film.

  16. Changes in viral and bacterial communities during the ice-melting season in the coastal Arctic (Kongsfjorden, Ny-Ålesund).

    Science.gov (United States)

    De Corte, Daniele; Sintes, Eva; Yokokawa, Taichi; Herndl, Gerhard J

    2011-07-01

    Microbial communities in Arctic coastal waters experience dramatic changes in environmental conditions during the spring to summer transition period, potentially leading to major variations in the relationship between viral and prokaryotic communities. To document these variations, a number of physico-chemical and biological parameters were determined during the ice-melting season in the coastal Arctic (Kongsfjorden, Ny-Ålesund, Spitsbergen). The bacterial and viral abundance increased during the spring to summer transition period, probably associated to the increase in temperature and the development of a phytoplankton bloom. The increase in viral abundance was less pronounced than the increase in prokaryotic abundance; consequently, the viral to prokaryotic abundance ratio decreased. The bacterial and viral communities were stratified as determined by Automated Ribosomal Intergenic Spacer Analysis and Randomly Amplified Polymorphic DNA-PCR respectively. Both the bacterial and viral communities were characterized by a relatively low number of operational taxonomic units (OTUs). Despite the apparent low complexity of the bacterial and viral communities, the link between these two communities was weak over the melting season, as suggested by the different trends of prokaryotic and viral abundance during the sampling period. This weak relationship between the two communities might be explained by UV radiation and suspended particles differently affecting the viruses and prokaryotes in the coastal Arctic during this period. Based on our results, we conclude that the viral and bacterial communities in the Arctic were strongly affected by the variability of the environmental conditions during the transition period between spring and summer.

  17. The Sensible Heat Flux in the Course of the Year at Ny-Ålesund, Svalbard: Characteristics of Eddy Covariance Data and Corresponding Model Results

    Directory of Open Access Journals (Sweden)

    Georg Jocher

    2015-01-01

    Full Text Available In this paper we present one year of meteorological and flux measurements obtained near Ny-Ålesund, Spitsbergen. Fluxes are derived by the eddy covariance method and by a hydrodynamic model approach (HMA as well. Both methods are compared and analyzed with respect to season and mean wind direction. Concerning the wind field we find a clear distinction between 3 prevailing regimes (which have influence on the flux behavior mainly caused by the topography at the measurement site. Concerning the fluxes we find a good agreement between the HMA and the eddy covariance method in cases of turbulent mixing in summer but deviations at stable conditions, when the HMA almost always shows negative fluxes. Part of the deviation is based on a dependence of HMA fluxes on friction velocity and the influence of the molecular boundary layer. Moreover, the flagging system of the eddy covariance software package TK3 is briefly revised. A new quality criterion for the use of fluxes obtained by the eddy covariance method, which is based on integral turbulence characteristics, is proposed.

  18. The influence of accuracy, grid size, and interpolation method on the hydrological analysis of LiDAR derived dems: Seneca Nation of Indians, Irving NY

    Science.gov (United States)

    Clarkson, Brian W.

    Light Detection and Ranging (LiDAR) derived Digital Elevation Models (DEMs) provide accurate, high resolution digital surfaces for precise topographic analysis. The following study investigates the accuracy of LiDAR derived DEMs by calculating the Root Mean Square Error (RMSE) of multiple interpolation methods with grid cells ranging from 0.5 to 10-meters. A raster cell with smaller dimensions will drastically increase the amount of detail represented in the DEM by increasing the number of elevation values across the study area. Increased horizontal resolutions have raised the accuracy of the interpolated surfaces and the contours generated from the digitized landscapes. As the raster grid cells decrease in size, the level of detail of hydrological processes will significantly improve compared to coarser resolutions including the publicly available National Elevation Datasets (NEDs). Utilizing a LiDAR derived DEM with the lowest RMSE as the 'ground truth', watershed boundaries were delineated for a sub-basin of the Clear Creek Watershed within the territory of the Seneca Nation of Indians located in Southern Erie County, NY. An investigation of the watershed area and boundary location revealed considerable differences comparing the results of applying different interpretation methods on DEM datasets of different horizontal resolutions. Stream networks coupled with watersheds were used to calculate peak flow values for the 10-meter NEDs and LiDAR derived DEMs.

  19. Distribution and transfer pattern of Polychlorinated Biphenyls (PCBs) among the selected environmental media of Ny-Ålesund, the Arctic: As a case study.

    Science.gov (United States)

    Zhang, Peng; Ge, Linke; Gao, Hui; Yao, Ting; Fang, Xiaodan; Zhou, Chuanguang; Na, Guangshui

    2014-12-15

    Polychlorinated Biphenyls (PCBs) were analyzed in multi-environmental samples collected from Ny-Ålesund, the Arctic to explore their legacy and transfer patterns. PCBs were ubiquitously in the environmental media, within the ranges of 1.73-6.27 and 9.18-141.1 pg m(-3) in vapor and aerosol, 2.76-10.8, 3.09-8.32, 22.5-56.3, 35.4-51.4 and 31.8-39.6 ng g(-1) (dry weight) in soil, sediment, plant, bird guano and reindeer faeces, respectively. The spatial distribution patterns exhibited a general southward decline in soil and sediment from the bay entrance to the inner bay. The concentration ratios of plants to soil inferred that no distinguished selective adsorption of PCBs congeners by plants existed and PCBs were mainly attributed to the air deposition on plant surface. The fugacity ratios among vapor, aerosol and soil indicted that the equilibrium status has not been reached and the net transferring direction was air-soil.

  20. Building America Case Study: Apartment Compartmentalization with an Aerosol-Based Sealing Process - Queens, NY; Technology Solutions for New and Existing Homes, Energy Efficiency & Renewable Energy (EERE)

    Energy Technology Data Exchange (ETDEWEB)

    None

    2015-07-01

    Air sealing of building enclosures is a difficult and time-consuming process. Current methods in new construction require laborers to physically locate small and sometimes large holes in multiple assemblies and then manually seal each of them. The innovation demonstrated under this research study was the automated air sealing and compartmentalization of buildings through the use of an aerosolized sealant, developed by the Western Cooling Efficiency Center at University of California Davis.
    CARB sought to demonstrate this new technology application in a multifamily building in Queens, NY. The effectiveness of the sealing process was evaluated by three methods: air leakage testing of overall apartment before and after sealing, point-source testing of individual leaks, and pressure measurements in the walls of the target apartment during sealing. Aerosolized sealing was successful by several measures in this study. Many individual leaks that are labor-intensive to address separately were well sealed by the aerosol particles. In addition, many diffuse leaks that are difficult to identify and treat were also sealed. The aerosol-based sealing process resulted in an average reduction of 71% in air leakage across three apartments and an average apartment airtightness of 0.08 CFM50/SF of enclosure area.

  1. Long-range transport of atmospheric lead reaching Ny-Ålesund: Inter-annual and seasonal variations of potential source areas

    Science.gov (United States)

    Bazzano, Andrea; Cappelletti, David; Udisti, Roberto; Grotti, Marco

    2016-08-01

    Atmospheric particulate matter (PM10) was collected at Ny-Ålesund (Svalbard Islands, Norwegian Arctic) during spring and summer from 2010 to 2014 and analysed for lead content, enrichment factor and isotopic composition (208Pb/206Pb and 207Pb/206Pb). It was found that atmospheric lead was mainly of anthropogenic origin and neither its mean concentration, nor its isotopic composition was subjected to significant inter-annual differences (p-value > 0.1). Seasonal differences in both lead content and isotopic compositions occurred (p-value mining and smelting activities in the Rudny Altay region (Central Eurasia), as well as industrial emission in north-eastern North America, were the main sources of atmospheric lead in spring and summer, respectively. During 2013, no significant differences between the two seasons were found (p-value > 0.3), showing a prolonged influence of Eurasian sources also in summer. The results obtained by the Pb isotopic composition were corroborated by a back-trajectory cluster analysis of air-masses reaching the sampling site.

  2. Impacts of Changing Agri-Environmental Policy on Countryside Conservation: A Report of Focus Groups Held in Association with Skaneateles Lake Watershed, Skaneateles, NY, USA, and High Weald Area of Outstanding Natural Beauty, Goudhurst, Kent, England, UK

    OpenAIRE

    Gross, David; Bills, Nelson L.

    2003-01-01

    Utilizing area-based agri-environmental programs, our work involves focus groups and interviews with program managers, landowners, and elected officials to assess the impact of on-farm managerial interventions on broader countryside conservation issues. Initially, two areas were compared: The Skaneateles Lake Watershed Agricultural Program (NY) and the High Weald Land Management Initiative (England). The organizing principle for this research is that the British experience with countryside ma...

  3. Vaccination with NY-ESO-1 protein and CpG in Montanide induces integrated antibody/Th1 responses and CD8 T cells through cross-priming.

    Science.gov (United States)

    Valmori, Danila; Souleimanian, Naira E; Tosello, Valeria; Bhardwaj, Nina; Adams, Sylvia; O'Neill, David; Pavlick, Anna; Escalon, Juliet B; Cruz, Crystal M; Angiulli, Angelica; Angiulli, Francesca; Mears, Gregory; Vogel, Susan M; Pan, Linda; Jungbluth, Achim A; Hoffmann, Eric W; Venhaus, Ralph; Ritter, Gerd; Old, Lloyd J; Ayyoub, Maha

    2007-05-22

    The use of recombinant tumor antigen proteins is a realistic approach for the development of generic cancer vaccines, but the potential of this type of vaccines to induce specific CD8(+) T cell responses, through in vivo cross-priming, has remained unclear. In this article, we report that repeated vaccination of cancer patients with recombinant NY-ESO-1 protein, Montanide ISA-51, and CpG ODN 7909, a potent stimulator of B cells and T helper type 1 (Th1)-type immunity, resulted in the early induction of specific integrated CD4(+) Th cells and antibody responses in most vaccinated patients, followed by the development of later CD8(+) T cell responses in a fraction of them. The correlation between antibody and T cell responses, together with the ability of vaccine-induced antibodies to promote in vitro cross-presentation of NY-ESO-1 by dendritic cells to vaccine-induced CD8(+) T cells, indicated that elicitation of NY-ESO-1-specific CD8(+) T cell responses by cross-priming in vivo was associated with the induction of adequate levels of specific antibodies. Together, our data provide clear evidence of in vivo cross-priming of specific cytotoxic T lymphocytes by a recombinant tumor antigen vaccine, underline the importance of specific antibody induction for the cross-priming to occur, and support the use of this type of formulation for the further development of efficient cancer vaccines.

  4. 2014 iAREA campaign on aerosol in Spitsbergen - Part 2: Optical properties from Raman-lidar and in-situ observations at Ny-Ålesund

    Science.gov (United States)

    Ritter, C.; Neuber, R.; Schulz, Alexander; Markowicz, K. M.; Stachlewska, I. S.; Lisok, J.; Makuch, P.; Pakszys, P.; Markuszewski, P.; Rozwadowska, A.; Petelski, T.; Zielinski, T.; Becagli, S.; Traversi, R.; Udisti, R.; Gausa, M.

    2016-09-01

    In this work multi wavelength Raman lidar data from Ny-Ålesund, Spitsbergen have been analysed for the spring 2014 Arctic haze season, as part of the iAREA campaign. Typical values and probability distributions for aerosol backscatter, extinction and depolarisation, the lidar ratio and the color ratio for 4 different altitude intervals within the troposphere are given. These quantities and their dependencies are analysed and the frequency of altitude-dependent observed aerosol events are given. A comparison with ground-based size distribution and chemical composition is performed. Hence the aim of this paper is to provide typical and statistically meaningful properties of Arctic aerosol, which may be used in climate models or to constrain the radiative forcing. We have found that the 2014 season was only moderately polluted with Arctic haze and that sea salt and sulphate were the most dominant aerosol species. Moreover the drying of an aerosol layer after cloud disintegration has been observed. Hardly any clear temporal evolution over the 4 week data set on Arctic haze is obvious with the exception of the extinction coefficient and the lidar ratio, which significantly decreased below 2 km altitude by end April. In altitudes between 2 and 5 km the haze season lasted longer and the aerosol properties were generally more homogeneous than closer to the surface. Above 5 km only few particles were found. The variability of the lidar ratio is discussed. It was found that knowledge of the aerosol's size and shape does not determine the lidar ratio. Contrary to shape and lidar ratio, there is a clear correlation between size and backscatter: larger particles show a higher backscatter coefficient.

  5. Soil pH is a Key Determinant of Soil Fungal Community Composition in the Ny-Ålesund Region, Svalbard (High Arctic).

    Science.gov (United States)

    Zhang, Tao; Wang, Neng-Fei; Liu, Hong-Yu; Zhang, Yu-Qin; Yu, Li-Yan

    2016-01-01

    This study assessed the fungal community composition and its relationships with properties of surface soils in the Ny-Ålesund Region (Svalbard, High Arctic). A total of thirteen soil samples were collected and soil fungal community was analyzed by 454 pyrosequencing with fungi-specific primers targeting the rDNA internal transcribed spacer (ITS) region. The following eight soil properties were analyzed: pH, organic carbon (C), organic nitrogen (N), ammonium nitrogen (NH4 (+)-N), silicate silicon (SiO4 (2-)-Si), nitrite nitrogen (NO2 (-)-N), phosphate phosphorus (PO4 (3-)-P), and nitrate nitrogen (NO3 (-)-N). A total of 57,952 reads belonging to 541 operational taxonomic units (OTUs) were found. of these OTUs, 343 belonged to Ascomycota, 100 to Basidiomycota, 31 to Chytridiomycota, 22 to Glomeromycota, 11 to Zygomycota, 10 to Rozellomycota, whereas 24 belonged to unknown fungi. The dominant orders were Helotiales, Verrucariales, Agaricales, Lecanorales, Chaetothyriales, Lecideales, and Capnodiales. The common genera (>eight soil samples) were Tetracladium, Mortierella, Fusarium, Cortinarius, and Atla. Distance-based redundancy analysis (db-rda) and analysis of similarities (ANOSIM) revealed that soil pH (p = 0.001) was the most significant factor in determining the soil fungal community composition. Members of Verrucariales were found to predominate in soils of pH 8-9, whereas Sordariales predominated in soils of pH 7-8 and Coniochaetales predominated in soils of pH 6-7. The results suggest the presence and distribution of diverse soil fungal communities in the High Arctic, which can provide reliable data for studying the ecological responses of soil fungal communities to climate changes in the Arctic.

  6. Chronostratigraphy of a salt marsh sediment core from North Cinder Island in the Town of Hempstead, Long Island, NY, using radiocarbon and pollen

    Science.gov (United States)

    Farmer, E. C.; Browne, J.; Peteet, D. M.; Cochran, K. K.; Heilbrun, C.; Chery, N.; LongJohn, T.; Mayo, J.; Ricigliano, V.

    2016-12-01

    A 122 cm long sediment core was collected from the salt marsh of North Cinder Island (73.6092W, 40.6097N), a small uninhabited island in Middle Bay between Oceanside and Point Lookout, in the Town of Hempstead, NY, on 2 July 2013, in order to investigate the age of the marsh and the history of trace metal pollution in the area. First, to determine the chronostratigraphy of the core, pollen counts were compared to radiocarbon measurements. Sediment samples at several depths in the core were analyzed for Pine, Oak, Hickory, Birch, Grass (S. alterniflora and S. patens), and Ragweed pollen. The concentration of Ragweed was below 3% in samples below 80cm, and greater than 7% in samples above 80cm. This proliferation of a disturbance species suggests that layers deeper than 81cm were deposited prior to widespread European settlement, sometime in the 1600s AD. Paired radiocarbon measurements on sieved fine sediment at 42-43 cm depth, however, match well with each other (their 1-sigma confidence intervals overlap), but suggest a calendar age between 932 and 997 years before present. Paired radiocarbon measurements from the 60-61 cm depth also match well with each other, but represent an age that is approximately 200 years younger. Additional paired radiocarbon measurements at 78-79 cm and 96-97 cm depths give older ages, as expected stratigraphically. Perhaps the reversal between 43 and 60 cm represents reworking of sediments in the marsh by tidal currents. Interestingly, root matter extracted from the sediment at the same depths gives radiocarbon ages that range from 600-1200 years younger. Perhaps the roots penetrate down through older sediment, or perhaps the fine sediment is comprised of recaptured sediment with lignin or other residual organic matter that is older because it is difficult to break down. This would explain the apparent contradiction between the radiocarbon dates on fine sediment and the younger pollen date at a deeper depth.

  7. Graphical genotyping as a method to map Ny (o,n)sto and Gpa5 using a reference panel of tetraploid potato cultivars.

    Science.gov (United States)

    van Eck, Herman J; Vos, Peter G; Valkonen, Jari P T; Uitdewilligen, Jan G A M L; Lensing, Hellen; de Vetten, Nick; Visser, Richard G F

    2017-03-01

    The method of graphical genotyping is applied to a panel of tetraploid potato cultivars to visualize haplotype sharing. The method allowed to map genes involved in virus and nematode resistance. The physical coordinates of the amount of linkage drag surrounding these genes are easily interpretable. Graphical genotyping is a visually attractive and easily interpretable method to represent genetic marker data. In this paper, the method is extended from diploids to a panel of tetraploid potato cultivars. Application of filters to select a subset of SNPs allows one to visualize haplotype sharing between individuals that also share a specific locus. The method is illustrated with cultivars resistant to Potato virus Y (PVY), while simultaneously selecting for the absence of the SNPs in susceptible clones. SNP data will then merge into an image which displays the coordinates of a distal genomic region on the northern arm of chromosome 11 where a specific haplotype is introgressed from the wild potato species S. stoloniferum (CPC 2093) carrying a gene (Ny (o,n)sto ) conferring resistance to two PVY strains, PVY(O) and PVY(NTN). Graphical genotyping was also successful in showing the haplotypes on chromosome 12 carrying Ry-f sto , another resistance gene derived from S. stoloniferum conferring broad-spectrum resistance to PVY, as well as chromosome 5 haplotypes from S. vernei, with the Gpa5 locus involved in resistance against Globodera pallida cyst nematodes. The image also shows shortening of linkage drag by meiotic recombination of the introgression segment in more recent breeding material. Identity-by-descent was found to be a requirement for using graphical genotyping, which is proposed as a non-statistical alternative method for gene discovery, as compared with genome-wide association studies. The potential and limitations of the method are discussed.

  8. Soil pH is a key determinant of soil fungal community composition in the Ny-Ålesund Region, Svalbard (High Arctic

    Directory of Open Access Journals (Sweden)

    Tao eZhang

    2016-02-01

    Full Text Available This study assessed the fungal community composition and its relationships with properties of surface soils in the Ny-Ålesund Region (Svalbard, High Arctic. A total of thirteen soil samples were collected and soil fungal community was analyzed by 454 pyrosequencing with fungi-specific primers targeting the rDNA internal transcribed spacer (ITS region. The following eight soil properties were analyzed: pH, organic carbon (C, organic nitrogen (N, ammonium nitrogen (NH4+-N, silicate silicon (SiO42--Si, nitrite nitrogen (NO2--N, phosphate phosphorus (PO43--P and nitrate nitrogen (NO3--N. A total of 57,952 reads belonging to 541 operational taxonomic units (OTUs were found. Of these OTUs, 343 belonged to Ascomycota, 100 to Basidiomycota, 31 to Chytridiomycota, 22 to Glomeromycota, 11 to Zygomycota, 10 to Rozellomycota, whereas 24 belonged to unknown fungi. The dominant orders were Helotiales, Verrucariales, Agaricales, Lecanorales, Chaetothyriales, Lecideales, and Capnodiales. The common genera (>8 soil samples were Tetracladium, Mortierella, Fusarium, Cortinarius, and Atla. Distance-based redundancy analysis (db-rda and analysis of similarities (ANOSIM revealed that soil pH (p=0.001 was the most significant factor in determining the soil fungal community composition. Members of Verrucariales were found to predominate in soils of pH 8-9, whereas Sordariales predominated in soils of pH 7-8 and Coniochaetales predominated in soil samples of pH 6-7. The results suggest the presence and distribution of diverse soil fungal communities in the High Arctic, which can provide reliable data for studying the ecological responses of soil fungal communities to climate changes in the Arctic.

  9. Simultaneous cytoplasmic and nuclear protein expression of melanoma antigen-A family and NY-ESO-1 cancer-testis antigens represents an independent marker for poor survival in head and neck cancer.

    Science.gov (United States)

    Laban, Simon; Atanackovic, Djordje; Luetkens, Tim; Knecht, Rainald; Busch, Chia-Jung; Freytag, Marcus; Spagnoli, Giulio; Ritter, Gerd; Hoffmann, Thomas K; Knuth, Alexander; Sauter, Guido; Wilczak, Waldemar; Blessmann, Marco; Borgmann, Kerstin; Muenscher, Adrian; Clauditz, Till S

    2014-09-01

    The prognosis of head and neck squamous cell carcinoma (HNSCC) patients remains poor. The identification of high-risk subgroups is needed for the development of custom-tailored therapies. The expression of cancer-testis antigens (CTAs) has been linked to a worse prognosis in other cancer types; however, their prognostic value in HNSCC is unclear because only few patients have been examined and data on CTA protein expression are sparse. A tissue microarray consisting of tumor samples from 453 HNSCC patients was evaluated for the expression of CTA proteins using immunohistochemistry. Frequency of expression and the subcellular expression pattern (nuclear, cytoplasmic, or both) was recorded. Protein expression of melanoma antigen (MAGE)-A family CTA, MAGE-C family CTA and NY-ESO-1 was found in approximately 30, 7 and 4% of tumors, respectively. The subcellular expression pattern in particular had a marked impact on the patients' prognosis. Median overall survival (OS) of patients with (i) simultaneous cytoplasmic and nuclear expression compared to (ii) either cytoplasmic or nuclear expression and (iii) negative patients was 23.0 versus 109.0 versus 102.5 months, for pan-MAGE (p < 0.0001), 46.6 versus 50.0 versus 109.0 for MAGE-A3/A4 (p = 0.0074) and 13.3 versus 50.0 versus 100.2 months for NY-ESO-1 (p = 0.0019). By multivariate analysis, these factors were confirmed as independent markers for poor survival. HNSCC patients showing protein expression of MAGE-A family members or NY-ESO-1 represent a subgroup with an extraordinarily poor survival. The development of immunotherapeutic strategies targeting these CTA may, therefore, be a promising approach to improve the outcome of HNSCC patients.

  10. Geomorphologic mapping in the Ny Ålesund area (Svalbard Island, Norway) for the analysis of geomorphologic effects on rock slopes induced by glacier retreat in climate sensitive High Arctic regions

    Science.gov (United States)

    Miccadei, Enrico; Piacentini, Tommaso; Casacchia, Ruggero; Sparapani, Roberto

    2014-05-01

    The geomorphological effects of glacial retreat, rapidly changing Arctic environments and consequent local temporary permafrost melting are several types of glacial and periglacial landforms (pingos, solifluction, drumlins, etc.) but also debris and rock falls, alluvial fan and glacial outwash development and scarp/slopes retreat and evolution. In this work we have realized a geomorphologic map of rockfalls, landslides, alluvial fans and the slopes and scarps of steep mountainsides in the Ny Ålesund area (Svalbard Island, Norway) focused on the analysis of rock falls as geomorphological effects of glacier retreat, permafrost degradation and higher temperatures on slope processes. The investigation is based on geological and geomorphological field survey, and remote sensing and aerial photo interpretation, The Ny Ålesund area landscape is characterized by rugged non-vegetated mountains only partially covered by glaciers, with steep flanks and rock scarps; the scarps are formed by different types of rocks (intrusive and effusive igneous rocks, marine sedimentary rocks); this landscape is highly affected by debris and rock falls (from scarps and slopes) forming wide talus slopes and by alluvial fan and fluvial outwash (from glaciers), which make the surface sedimentary cover of the island together with rock glaciers and moraine deposits and locally fluvial deposits. The work is focused on the comprehension of the role of different factors in inducing rock falls, alluvial fans, slope/scarps evolution in high geomorphological sensitivity environments (i.e. glacial, periglacial or mountain) including: orography, lithology, rock fracturation, morphostructural setting, meteorological context. The conclusions focus on the possible geomorphological hazards affecting the Ny Ålesund area.

  11. Ny klassifikation af pancreatitis acuta

    DEFF Research Database (Denmark)

    Hansen, Benny Østerbye; Schmidt, Palle Nordblad

    2011-01-01

    The course of acute pancreatitis is in the initial phase dominated by a systemic inflammatory response, later by local complications. A new classification defines three specific types of pancreatitis: 1) interstitial oedematous pancreatitis and 2) necrotizing pancreatitis with pancreatic...

  12. Ny software helt uden programmering

    DEFF Research Database (Denmark)

    Kindler, Ekkart

    2010-01-01

    Fremtiden er modelbaseret softwareudvikling. Men er det bare en våd drøm, eller er det realistisk at tro, at man kan lave software uden programmering?......Fremtiden er modelbaseret softwareudvikling. Men er det bare en våd drøm, eller er det realistisk at tro, at man kan lave software uden programmering?...

  13. Ny verdensrekord for DTU Roadrunners

    DEFF Research Database (Denmark)

    Lassen, Lisbeth

    2015-01-01

    Økobilen har slået sin egen verdensrekord fra 2013 ved at køre hvad der svarer til 665 km på en liter benzin til Shell Eco – Marathon i Rotterdam lørdag d. 23. maj i kategorien Urban Concept.......Økobilen har slået sin egen verdensrekord fra 2013 ved at køre hvad der svarer til 665 km på en liter benzin til Shell Eco – Marathon i Rotterdam lørdag d. 23. maj i kategorien Urban Concept....

  14. Coastal Analysis, Nassau,NY

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Coastal study as defined in FEMA Guides and Specifications, Appendix D: Guidance for coastal Flooding Analyses and Mapping submitted as a result of a coastal study....

  15. Underdanmark i ny dansk prosa

    DEFF Research Database (Denmark)

    Gemzøe, Anker

    2016-01-01

    were among the best reviewed and most read during that year: four novels and a poetry collection, in this context considered a sort of verse novel. Which literary and ideological tendencies in this renewed preoccupation with Sub-Denmark are highlighted in this process? Do they correspond to our...... the autobiographically based family novel, portraying an upbringing in a dysfunctional family at the bottom of society – thereby focussing on the world of childhood....

  16. På vej mod ny skolemad

    DEFF Research Database (Denmark)

    Nielsen, Morten Kromann; Høyrup, Jon Frederik

    I forbindelse med budgetforliget 2008 vedtog Københavns Kommune at indføre et nyt samlet skolemadskoncept for alle kommunens skoler. Rapporten er en evaluering af dette koncept. Rapporten er samtidig afslutningen på et omfangsrigt antropologisk arbejde, der begyndte i efteråret 2008 og blev afslu...

  17. Bange anelser om ny anbringelsesreform

    DEFF Research Database (Denmark)

    Kildedal, Karin

    2004-01-01

    Artiklen omhandler en analyse af den anbringelsereform, der er under behandling i folketinget i 2004. Analysen problematiserer, at staten fortsat prøver at kvalificere det sociale arbejdes praksis gennem lovgivning....

  18. Kraftig fremgang til Ny Alliance

    DEFF Research Database (Denmark)

    Thomsen, Søren Risbjerg

    2007-01-01

    NYHED: Nyt vejet gennemsnit giver Khader tre ekstra mandater på valgkampens første fire dage. Udgivelsesdato: 28. oktober......NYHED: Nyt vejet gennemsnit giver Khader tre ekstra mandater på valgkampens første fire dage. Udgivelsesdato: 28. oktober...

  19. Photosynthetic and growth response of sugar maple (Acer saccharum Marsh.) mature trees and seedlings to calcium, magnesium, and nitrogen additions in the Catskill Mountains, NY, USA

    Science.gov (United States)

    Momen, Bahram; Behling, Shawna J; Lawrence, Gregory B.; Sullivan, Joseph H

    2015-01-01

    Decline of sugar maple in North American forests has been attributed to changes in soil calcium (Ca) and nitrogen (N) by acidic precipitation. Although N is an essential and usually a limiting factor in forests, atmospheric N deposition may cause N-saturation leading to loss of soil Ca. Such changes can affect carbon gain and growth of sugar maple trees and seedlings. We applied a 22 factorial arrangement of N and dolomitic limestone containing Ca and Magnesium (Mg) to 12 forest plots in the Catskill Mountain region of NY, USA. To quantify the short-term effects, we measured photosynthetic-light responses of sugar maple mature trees and seedlings two or three times during two summers. We estimated maximum net photosynthesis (An-max) and its related light intensity (PAR at An-max), apparent quantum efficiency (Aqe), and light compensation point (LCP). To quantify the long-term effects, we measured basal area of living mature trees before and 4 and 8 years after treatment applications. Soil and foliar chemistry variables were also measured. Dolomitic limestone increased Ca, Mg, and pH in the soil Oe horizon. Mg was increased in the B horizon when comparing the plots receiving N with those receiving CaMg. In mature trees, foliar Ca and Mg concentrations were higher in the CaMg and N+CaMg plots than in the reference or N plots; foliar Ca concentration was higher in the N+CaMg plots compared with the CaMg plots, foliar Mg was higher in the CaMg plots than the N+CaMg plots; An-max was maximized due to N+CaMg treatment; Aqe decreased by N addition; and PAR at An-max increased by N or CaMg treatments alone, but the increase was maximized by their combination. No treatment effect was detected on basal areas of living mature trees four or eight years after treatment applications. In seedlings, An-max was increased by N+CaMg addition. The reference plots had an open herbaceous layer, but the plots receiving N had a dense monoculture of common woodfern in the

  20. Photosynthetic and Growth Response of Sugar Maple (Acer saccharum Marsh. Mature Trees and Seedlings to Calcium, Magnesium, and Nitrogen Additions in the Catskill Mountains, NY, USA.

    Directory of Open Access Journals (Sweden)

    Bahram Momen

    Full Text Available Decline of sugar maple in North American forests has been attributed to changes in soil calcium (Ca and nitrogen (N by acidic precipitation. Although N is an essential and usually a limiting factor in forests, atmospheric N deposition may cause N-saturation leading to loss of soil Ca. Such changes can affect carbon gain and growth of sugar maple trees and seedlings. We applied a 22 factorial arrangement of N and dolomitic limestone containing Ca and Magnesium (Mg to 12 forest plots in the Catskill Mountain region of NY, USA. To quantify the short-term effects, we measured photosynthetic-light responses of sugar maple mature trees and seedlings two or three times during two summers. We estimated maximum net photosynthesis (An-max and its related light intensity (PAR at An-max, apparent quantum efficiency (Aqe, and light compensation point (LCP. To quantify the long-term effects, we measured basal area of living mature trees before and 4 and 8 years after treatment applications. Soil and foliar chemistry variables were also measured. Dolomitic limestone increased Ca, Mg, and pH in the soil Oe horizon. Mg was increased in the B horizon when comparing the plots receiving N with those receiving CaMg. In mature trees, foliar Ca and Mg concentrations were higher in the CaMg and N+CaMg plots than in the reference or N plots; foliar Ca concentration was higher in the N+CaMg plots compared with the CaMg plots, foliar Mg was higher in the CaMg plots than the N+CaMg plots; An-max was maximized due to N+CaMg treatment; Aqe decreased by N addition; and PAR at An-max increased by N or CaMg treatments alone, but the increase was maximized by their combination. No treatment effect was detected on basal areas of living mature trees four or eight years after treatment applications. In seedlings, An-max was increased by N+CaMg addition. The reference plots had an open herbaceous layer, but the plots receiving N had a dense monoculture of common woodfern in the

  1. The role of bromine and chlorine chemistry for arctic ozone depletion events in Ny-Ålesund and comparison with model calculations

    Directory of Open Access Journals (Sweden)

    M. Martinez

    Full Text Available During the Arctic Tropospheric Ozone Chemistry (ARCTOC campaigns at Ny-Ålesund, Spitsbergen, the role of halogens in the depletion of boundary layer ozone was investigated. In spring 1995 and 1996 up to 30 ppt bromine monoxide were found whenever ozone decreased from normal levels of about 40 ppb. Those main trace gases and others were specifically followed in the UV-VIS spectral region by differential optical absorption spectroscopy (DOAS along light paths running between 20 and 475 m a.s.l.. The daily variation of peroxy radicals closely followed the ozone photolysis rate J(O3(O1D in the absence of ozone depletion most of the time. However, during low ozone events this close correlation was no longer found because the measurement of radicals by chemical amplification (CA turned out to be sensitive to peroxy radicals and ClOx. Large CA signals at night can sometimes definitely be assigned to ClOx and reached up to 2 ppt. Total bromine and iodine were both stripped quantitatively from air by active charcoal traps and measured after neutron activation of the samples. Total bromine increased from background levels of about 15 ppt to a maximum of 90 ppt during an event of complete ozone depletion. For the spring season a strong source of bromine is identified in the pack ice region according to back trajectories. Though biogenic emission sources cannot be completely ruled out, a primary activation of halogenides by various oxidants seems to initiate an efficient autocatalytic process, mainly driven by ozone and light, on ice and perhaps on aerosols. Halogenides residing on pack ice surfaces are continuously oxidised by hypohalogenous acids releasing bromine and chlorine into the air. During transport and especially above open water this air mixes with upper layer pristine air. As large quantities of bromine, often in the form of BrO, have been observed at polar sunrise also around Antarctica, its release

  2. Sand on the move: Post Hurricane Sandy analysis of the coastal sediment budget and bedform migration at Jones Inlet, Long Island, NY

    Science.gov (United States)

    Bales, M. K.; Goff, J. A.; Austin, J. A.; Flood, R. D.; Christensen, B. A.; Browne, C. M.; Saustrup, S.

    2013-12-01

    Hurricane Sandy struck the Northeast coast of the United States on October 29, 2012. Although sustained winds were downgraded to ~70 kts upon landfall, the vast area of the storm along with the direction of impact resulted in major storm-surge flooding and damage, as well as significant changes to the morphology of the coast-line, altering the characteristics of major barrier islands, inlets, and estuaries. A January 2013 post-storm survey aboard the R/V Pritchard, conducted by the University of Texas Institute for Geophysics, Stony Brook University, and Adelphi University, sought to investigate the impact of this post-tropical cyclone on the southwestern coast of Long Island, NY which sustained storm surges of up to 4 m above normal sea level. The objective of this project is to gain insight on the sedimentological volume changes and bathymetrical alterations made on the seafloor within Jones Inlet and the immediate estuaries behind Jones Beach and Long Beach Island. By studying these changes we hope to gain a better understanding of how large cyclonic storms alter sediment volumes and seafloor topography within major inlets and estuarine systems. These modifications can be observed in post-storm multibeam swath bathymetry and backscatter when compared to similar pre-storm data collected in 2010 by Stony Brook University. Post-storm CHIRP seismic reflection data were also collected, in order to define stratigraphic geometries, as well as grab samples to determine grain size distributions and ground truth for the backscatter data. The acoustic reflections imaged in the CHIRP data allow basal reflectors and dominate horizons to be traced throughout the inlet and estuaries. Our analysis focuses on (1) defining and quantifying areas of deposition and erosion from before-and-after bathymetry data; (2) comparing bedform patterns and grain size distributions from before-and-after backscatter and grab sample analysis; and (3) defining stratal geometries of the shallow

  3. Arctic aerosol life cycle: linking aerosol size distributions observed between 2000 and 2010 with air mass transport and precipitation at Zeppelin station, Ny-Ålesund, Svalbard

    Directory of Open Access Journals (Sweden)

    P. Tunved

    2012-11-01

    Full Text Available In this study we present a qualitative and quantitative assessment of more the 10 yr of aerosol number size distribution data observed in the Arctic environment (Mt Zeppelin (78°56' N, 11°53' E, 474 m a.s.l., Ny Ålesund, Svalbard. We provide statistics on both seasonal and diurnal characteristics of the aerosol observations and conclude that the Arctic aerosol number size distribution and auxiliary parameters such as integral mass and surface have a very pronounced seasonal variation. This seasonal variation seems to be controlled by both dominating source as well as meteorological conditions in general. In principle, three distinctly different periods can be identified during the Arctic year: the haze period characterized by a dominating accumulation mode aerosol (March–May followed by the sunlit summer period with low abundance of accumulation mode particles but high concentration of small particles which likely are recently and locally formed (June–August. The rest of the year is characterized by comparably low concentration of accumulation mode particles and negligible abundance of ultra fine particles (September–February. Minimum aerosol mass and number concentration is usually observed during September/October. We further show that the transition between the different regimes is fast, suggesting rapid change in conditions defining their appearance. A source climatology based on trajectory analysis is provided and it is shown that there is a strong seasonality of dominating source areas, with dominance of Eurasia during the autumn-winter period and dominance of North Atlantic air during the summer months. We also show that new particle formation events seem to be a rather common phenomenon during the Arctic summer, and this is the result of both photochemical production of nucleating/condensing species and low condensation sink. It is also suggested that wet removal play a key role in defining the Arctic aerosol year, and plays a

  4. On the acidic metabolite characterization and degradation process of the phenanthrene by Pseudomonas aeruginosa NY3%铜绿假单胞菌降解菲的产物鉴定及代谢过程

    Institute of Scientific and Technical Information of China (English)

    沈小娟; 聂麦茜; 葛碧洲; 樊瑜; 王菲; 常虹

    2012-01-01

    This paper deals with a study on the acidic metabolite characterization and degradation process of the phenanthrene by means of Pseudomonas aeruginosa NY3. As is known, though there has been a lot of articles published on the study of metabolites, yet little has been reported on the biodegradation pathways of polycyclic aromatic hydrocarbons by pseudomonas aeruginosa. Here in this paper we would like to report the typical acidic metabolites of phenanthrene and its degrading pathway by P. aeruginosa NY3, which can help to metabolize hydrophobic compounds quickly. P. aeruginosa NY3, as a matter of fact, could grow on the phenanthrene well and its metabolites such as a-naphthol, salicylic acid, benzoic acid in regard with its sole carbon source and energy. GC ~ MS data indicate that it is possible to find five major metabolites of phenanthrene as the bacteria grow. They are 1-hydroxy-2-naphthoic acid, 2-hydroxy-2-carboxylic acid, a-naphthol, o-hydroxyphenyl carbonyl acid, salicylic acid, and benzoic acid. Based on the analysis of metabolites, the biodegradation pathway can be deduced - First of all, the phenanthrene can be made transformed into l-hydroxy-2-naphthoic acid after a series of oxidative metabolism steps by P. aeruginosa NY3, and later into a-naphthol through decavboxylation. Other metabolites that can be transformed from phenanthrene into l-hydroxy-2-naphthoic acid can be metabolized so fast that it won' t be possible to be found in our experiments, though it would be possible to find one-aromatic-ring metabolites like o-hydroxyphenyl carbonyl acid, salicylic acid, benzoic acid were detected was the evidence of further transformation of a-naphthol. However, it wouldn't be possible to find dihydroxy-naphthalene, dihydroxybenzene and other polyphenols could as the metabolites of phenanthrene by pseudomonas aeruginosa NY3, while there were greater amount of metabolites which can be characterized as ortho-hydroxyl carboxyl compounds, such as 1-hydroxy-2-naph

  5. Differences in Arctic and Antarctic PSC occurrence as observed by lidar in Ny-Ålesund (79° N, 12° E and McMurdo (78° S, 167° E

    Directory of Open Access Journals (Sweden)

    M. Maturilli

    2005-01-01

    Full Text Available The extent of springtime Arctic ozone loss does not reach Antarctic ``ozone hole'' dimensions because of the generally higher temperatures in the northern hemisphere vortex and consequent less polar stratospheric cloud (PSC particle surface for heterogeneous chlorine activation. Yet, with increasing greenhouse gases stratospheric temperatures are expected to further decrease. To infer if present Antarctic PSC occurrence can be applied to predict future Arctic PSC occurrence, lidar observations from McMurdo station (78° S, 167° E and NyÅlesund (79° N, 12° E have been analysed for the 9 winters between 1995 (1995/1996 and 2003 (2003/2004. Although the statistics may not completely cover the overall hemispheric PSC occurrence, the observations are considered to represent the main synoptic cloud features as both stations are mostly situated in the centre or at the inner edge of the vortex. Since the focus is set on the occurrence frequency of solid and liquid particles, the analysis has been restricted to volcanic aerosol free conditions. In McMurdo, by far the largest part of PSC observations is associated with NAT PSCs. The observed persistent background of NAT particles and their potential ability to cause denoxification and irreversible denitrification is presumably more important to Antarctic ozone chemistry than the scarcely observed ice PSCs. Meanwhile in Ny-Ålesund, ice PSCs have never been observed, while solid NAT and liquid STS clouds both occur in large fraction. Although they are also found solely, the majority of observations reveals solid and liquid particle layers in the same profile. For the Ny-Ålesund measurements, the frequent occurrence of liquid PSC particles yields major significance in terms of ozone chemistry, as their chlorine activation rates are more efficient. The relationship between temperature, PSC formation, and denitrification is nonlinear and the McMurdo and Ny-Ålesund PSC observations imply that for

  6. Vaccination of stage III/IV melanoma patients with long NY-ESO-1 peptide and CpG-B elicits robust CD8+ and CD4+ T-cell responses with multiple specificities including a novel DR7-restricted epitope

    Science.gov (United States)

    Baumgaertner, P.; Costa Nunes, C.; Cachot, A.; Maby-El Hajjami, H.; Cagnon, L.; Braun, M.; Derré, L.; Rivals, J.-P.; Rimoldi, D.; Gnjatic, S.; Abed Maillard, S.; Marcos Mondéjar, P.; Protti, M. P.; Romano, E.; Michielin, O.; Romero, P.; Speiser, D. E.; Jandus, C.

    2016-01-01

    ABSTRACT Long synthetic peptides and CpG-containing oligodeoxynucleotides are promising components for cancer vaccines. In this phase I trial, 19 patients received a mean of 8 (range 1–12) monthly vaccines s.c. composed of the long synthetic NY-ESO-179–108 peptide and CpG-B (PF-3512676), emulsified in Montanide ISA-51. In 18/18 evaluable patients, vaccination induced antigen-specific CD8+ and CD4+ T-cell and antibody responses, starting early after initiation of immunotherapy and lasting at least one year. The T-cells responded antigen-specifically, with strong secretion of IFNγ and TNFα, irrespective of patients' HLAs. The most immunogenic regions of the vaccine peptide were NY-ESO-189–102 for CD8+ and NY-ESO-183–99 for CD4+ T-cells. We discovered a novel and highly immunogenic epitope (HLA-DR7/NY-ESO-187–99); 7/7 HLA-DR7+ patients generated strong CD4+ T-cell responses, as detected directly ex vivo with fluorescent multimers. Thus, vaccination with the long synthetic NY-ESO-179–108 peptide combined with the strong immune adjuvant CpG-B induced integrated, robust and functional CD8+ and CD4+ T-cell responses in melanoma patients, supporting the further development of this immunotherapeutic approach. PMID:27853637

  7. Vaccination of stage III/IV melanoma patients with long NY-ESO-1 peptide and CpG-B elicits robust CD8(+) and CD4(+) T-cell responses with multiple specificities including a novel DR7-restricted epitope.

    Science.gov (United States)

    Baumgaertner, P; Costa Nunes, C; Cachot, A; Maby-El Hajjami, H; Cagnon, L; Braun, M; Derré, L; Rivals, J-P; Rimoldi, D; Gnjatic, S; Abed Maillard, S; Marcos Mondéjar, P; Protti, M P; Romano, E; Michielin, O; Romero, P; Speiser, D E; Jandus, C

    2016-01-01

    Long synthetic peptides and CpG-containing oligodeoxynucleotides are promising components for cancer vaccines. In this phase I trial, 19 patients received a mean of 8 (range 1-12) monthly vaccines s.c. composed of the long synthetic NY-ESO-179-108 peptide and CpG-B (PF-3512676), emulsified in Montanide ISA-51. In 18/18 evaluable patients, vaccination induced antigen-specific CD8(+) and CD4(+) T-cell and antibody responses, starting early after initiation of immunotherapy and lasting at least one year. The T-cells responded antigen-specifically, with strong secretion of IFNγ and TNFα, irrespective of patients' HLAs. The most immunogenic regions of the vaccine peptide were NY-ESO-189-102 for CD8(+) and NY-ESO-183-99 for CD4(+) T-cells. We discovered a novel and highly immunogenic epitope (HLA-DR7/NY-ESO-187-99); 7/7 HLA-DR7(+) patients generated strong CD4(+) T-cell responses, as detected directly ex vivo with fluorescent multimers. Thus, vaccination with the long synthetic NY-ESO-179-108 peptide combined with the strong immune adjuvant CpG-B induced integrated, robust and functional CD8(+) and CD4(+) T-cell responses in melanoma patients, supporting the further development of this immunotherapeutic approach.

  8. Tracing the Early Developments of Tibetan Epistemological Categories in Rngog Blo ldan shes rab's (1059-1109) Concise Guide to theNyāyabinduṭīkā1

    Institute of Scientific and Technical Information of China (English)

    Pascale Hugon

    2013-01-01

    AbstrAct The recent publication of theBka' gdams gsung 'bum collection is opening the way for new research in the field of Tibetan Buddhist epistemology by giving us access to some of the earliest indigenous compositions in the domain. This paper focuses on one of the two recovered epistemological works by the great scholar and translator Rngog Blo ldan shes rab (1059-1109): his concise guide to theNyāyabinduṭīkā, a commentary composed by Dharmottara (8th c.) on Dharmakīrti's (7th c. or earlier)Nyāyabindu. The first part of my paper provides a general introduction to Rngog Blo ldan shes rab's concise guide, which I am in the process of editing and translating. The description of the manuscript and a detailed presentation of the contents of the text will be provided in the introduction to this forthcoming study. I concentrate here on its place within Rngog Blo ldan shes rab's contributions as a translator and exegete and in relation with the spread of Dharmakīrti's works in Tibet. I discuss in particular the circumstances of its composition, its intended function and potential impact. In the second part of the paper I show how such

  9. Atmospheric black carbon concentrations in North America, ~1100 to 2005. Liaquat Husain and Tanveer Ahmed Wadsworth Center, New York State Department of Health, Albany, NY 12201-0509, USA, and Department of Environmental Health Sciences, School of Public Health, State University of New York, Albany, NY, 12201-0509, USA

    Science.gov (United States)

    Husain, L.

    2013-05-01

    Black carbon (BC) aerosols in the atmosphere absorb solar radiation, and cause heating of the atmosphere, may alter Earth's cloud cover, and impact precipitation cycle. The best estimate for radiative forcing from BC with 90% certainty is + 1.1 Wm-2 ( + 0.17 to 2.1 Wm-2), second only to that of CO2. A major uncertainty in the estimation is a lack of atmospheric BC data. Models are used to estimate BC emissions into the atmosphere. They have not been validated by field data. We report measurements of BC in the atmosphere, over a period of several centuries using lake sediments. Retrieval of such records provides an invaluable source for understanding changes in the atmosphere with time. Numerous studies have been conducted with such an objective. Owing to a lack of knowledge of deposition rates of atmospheric aerosols into the lake sediments, a major shortcoming of these studies have been an inability to convert the measurements of chemical species in lake sediments into the atmosphere. We have developed a technique to overcome this shortcoming by measuring black carbon in atmospheric aerosols, and in lake sediment cores. The concentrations of BC were determined in daily filters collected at Whiteface Mountain, NY, from 1978 to 2005. Cores from two lakes around Whiteface Mountain, NY, were collected. Cores were (1) sectioned in thin slices, (2) freeze dried, (3) dated using the 210Pb technique, (4) BC chemically separated, and (5) concentrations measured using the thermal-optical method. By The deposition rate of BC from the atmosphere to the lakes was determined by comparing the BC concentration in air and the sediments for the 1978 -2005 period. The deposition rate so determined was used to concert the BC in the sediment into the atmosphere for the ~1978 to ~1100 period. The BC concentrations for the industrial period, ~1850 was low but rapidly increased from ~1900, peaked ~1925, decreased very slowly ~ 1980, followed by a sharp decrease. Concentrations has

  10. Vaccination with a recombinant protein encoding the tumor-specific antigen NY-ESO-1 elicits an A2/157-165-specific CTL repertoire structurally distinct and of reduced tumor reactivity than that elicited by spontaneous immune responses to NY-ESO-1-expressing Tumors.

    Science.gov (United States)

    Bioley, Gilles; Guillaume, Philippe; Luescher, Immanuel; Bhardwaj, Nina; Mears, Gregory; Old, Lloyd; Valmori, Danila; Ayyoub, Maha

    2009-01-01

    In a recent vaccination trial assessing the immunogenicity of an NY-ESO-1 (ESO) recombinant protein administered with Montanide and CpG, we have obtained evidence that this vaccine induces specific cytolytic T lymphocytes (CTL) in half of the patients. Most vaccine-induced CTLs were directed against epitopes located in the central part of the protein, between amino acids 81 and 110. This immunodominant region, however, is distinct from another ESO CTL region, 157-165, that is a frequent target of spontaneous CTL responses in A2+ patients bearing ESO tumors. In this study, we have investigated the CTL responses to ESO 157-165 in A2+ patients vaccinated with the recombinant protein. Our data indicate that after vaccination with the protein, CTL responses to ESO 157-165 are induced in some, but not all, A2+ patients. ESO 157-165-specific CTLs induced by vaccination with the ESO protein were functionally heterogeneous in terms of tumor recognition and often displayed decreased tumor reactivity as compared with ESO 157-165-specific CTLs isolated from patients with spontaneous immune responses to ESO. Remarkably, protein-induced CTLs used T-cell receptors similar to those previously isolated from patients vaccinated with synthetic ESO peptides (Vbeta4.1) and distinct from those used by highly tumor-reactive CTLs isolated from patients with spontaneous immune responses (Vbeta1.1, Vbeta8.1, and Vbeta13.1). Together, these results demonstrate that vaccination with the ESO protein elicits a repertoire of ESO 157-165-specific CTLs bearing T-cell receptors that are structurally distinct from those of CTLs found in spontaneous immune responses to the antigen and that are heterogeneous in terms of tumor reactivity, being often poorly tumor reactive.

  11. 关于Diophantine方程(44n)x+(117n)y=(125n)z 的整数解%On the integral solutions of Diophantine equation (44n)x+(117n)y=(125n)z

    Institute of Scientific and Technical Information of China (English)

    鲁伟阳; 高丽; 郝虹斐

    2014-01-01

    在 Je´smanow´ıcz 猜想的基础上,利用初等方法证明了对任意的正整数 n, Diophantine方程(44n)x+(117n)y=(125n)z 仅有正整数解(x, y, z)=(2,2,2)。%In this paper, basing on Je´smanow´ıcz′ conjecture, it is proved that for any positive integral n the Diophantine equation (44n)x+(117n)y = (125n)z has only integral solution (x, y, z) = (2, 2, 2) by elementary method.

  12. Focusing of Dispersive Targets Using Synthetic Aperture Radar

    Science.gov (United States)

    1994-08-01

    Williams 1030 S Hwy AlA Patrick AFB OH 32925-3002 Distribution (cont’d) Phillips Lab Attn PL/WSR C Baum Attn PL/WSR J Berger Attn PL/WSR S A Blocher...Electrd Engrg Dept/WR1 Attn D M Bolle Attn S U Pillai 6 Metrotech Center Brooklyn NY 11201 Polytechnic Univ WRI & Dept of Physics Attn Mok-Ming Leung

  13. 平刺三阴交治疗原发性夜间遗尿42例%Forty-two cases of primary nocturnal enuresis treated with horizontal puncture on Sānyīnjiāo(三阴交SP 6)

    Institute of Scientific and Technical Information of China (English)

    袁红丽; 张建立; ZHANG Nan

    2010-01-01

    @@ The author had treated 42 cases of primary nocturnal enuresis with horizontal puncture on Sānyīnjiāo(三阴交SP 6) under her teacher's guidance from 2008 to 2010.The therapeutic outcomes are reported as follows.

  14. The development of a nanostructured, graded multilayer Cr-CrxNy-Cr1-xAlxN coating produced by pulsed closed field unbalanced magnetron sputtering (P-CFUBMS) for use in aluminum pressure die casting dies.

    Science.gov (United States)

    Lin, Jianliang; Mishra, Brajendra; Myers, Sterling; Ried, Peter; Moore, John J

    2009-06-01

    The main objective of this research is to design an optimized 'coating system' that extends die life by minimizing premature die failure. The concept of the multilayer coating system with desired combinations of different kinds of single-layer coatings was introduced. A pulsed closed field unbalanced magnetron sputtering (P-CFUBMS) deposition system has been used to deposit Cr-CrxNy-Cr1-xAlxN compositionally graded multilayer coating structures. In this study, three power law scenarios have been adopted to vary the aluminum concentration in the graded Cr1-xAlxN layer: (i) p = 1, the aluminum concentration was increased linearly in the Cr1-xAlxN layer. (ii) p = 0.2, the Cr1-xAlxN layer is an aluminum-rich graded layer, and (iii) p = 2, the Cr1-xAlxN layer is a chromium-rich graded layer. It was found that all the graded coatings exhibit lower residual stress and higher adhesion strength than the homogeneous Cr1-xAlxN (x = 0.585) film. However, different power law grading architectures have significant influence on the hardness and wear resistance of the films. When p = 2 and p = 1, the graded films exhibited relatively low hardness values (24 and 26 GPa respectively) and high COF (0.55 to 0.60). When p = 0.2 the graded film exhibited both high hardness (34 GPa) and good wear resistance (COF = 0.45) due to the structural consistency in the graded zone. The paper discusses the correlation between the pulsing parameters and coating architecture with the resulting nanostructure and tribological properties of this Cr-CrxNy-Cr1-xAlxN coating system.

  15. Vertical variability of aerosol single-scattering albedo and equivalent black carbon concentration based on in-situ and remote sensing techniques during the iAREA campaigns in Ny-Ålesund

    Science.gov (United States)

    Markowicz, K. M.; Ritter, C.; Lisok, J.; Makuch, P.; Stachlewska, I. S.; Cappelletti, D.; Mazzola, M.; Chilinski, M. T.

    2017-09-01

    This work presents a methodology for obtaining vertical profiles of aerosol single scattering properties based on a combination of different measurement techniques. The presented data were obtained under the iAREA (Impact of absorbing aerosols on radiative forcing in the European Arctic) campaigns conducted in Ny-Ålesund (Spitsbergen) during the spring seasons of 2015-2017. The retrieval uses in-situ observations of black carbon concentration and absorption coefficient measured by a micro-aethalometer AE-51 mounted onboard a tethered balloon, as well as remote sensing data obtained from sun photometer and lidar measurements. From a combination of the balloon-borne in-situ and the lidar data, we derived profiles of single scattering albedo (SSA) as well as absorption, extinction, and aerosol number concentration. Results have been obtained in an altitude range from about 400 m up to 1600 m a.s.l. and for cases with increased aerosol load during the Arctic haze seasons of 2015 and 2016. The main results consist of the observation of increasing values of equivalent black carbon (EBC) and absorption coefficient with altitude, and the opposite trend for aerosol concentration for particles larger than 0.3 μm. SSA was retrieved with the use of lidar Raman and Klett algorithms for both 532 and 880 nm wavelengths. In most profiles, SSA shows relatively high temporal and altitude variability. Vertical variability of SSA computed from both methods is consistent; however, some discrepancy is related to Raman retrieval uncertainty and absorption coefficient estimation from AE-51. Typically, very low EBC concentration in Ny-Ålesund leads to large error in the absorbing coefficient. However, SSA uncertainty for both Raman and Klett algorithms seems to be reasonable, e.g. SSA of 0.98 and 0.95 relate to an error of ±0.01 and ± 0.025, respectively.

  16. Ca2+ release from the endoplasmic reticulum of NY-ESO-1 specific T cells is modulated by the affinity of T cell receptor and by the use of the CD8 co-receptor

    Science.gov (United States)

    Stewart-Jones, Guillaume; Shepherd, Dawn; Bossi, Giovanna; Wooldridge, Linda; Hutchinson, Sarah L.; Sewell, Andrew K.; Griffiths, Gillian M.; van der Merwe, P. Anton; Jones, E. Yvonne; Galione, Antony; Cerundolo, Vincenzo

    2014-01-01

    Although several cancer immunotherapy strategies are currently based on the use of analog peptides and on the modulation of the TCR affinity of adoptively transferred T cells, it remains unclear whether tumor specific T cell activation by strong and weak TCR stimuli evoke different Ca2+ signatures from the Ca2+ intracellular stores and whether the amplitude of Ca2+ release from the ER can be further modulated by co-receptor binding to peptide/MHC complexes (pMHC). We here combined functional, structural and kinetic measurements to correlate the intensity of Ca2+ signals triggered by the stimulation of the 1G4 T cell clone specific to the tumor epitope NY-ESO-1157–165. Two analogs of the NY-ESO-1157–165 peptide, having similar affinity to HLA-A2 molecules, but a six-fold difference in binding affinity for the 1G4 TCR, resulted in different Ca2+ signals and T cell activation. 1G4 stimulation by the stronger stimulus emptied the ER of stored Ca2+, even in the absence of CD8 binding, resulting in sustained Ca2+ influx. In contrast, the weaker stimulus induced only partial emptying of stored Ca2+, resulting in significantly diminished and oscillatory Ca2+ signals, which was enhanced by CD8 binding. Our data define the range of TCR/pMHC affinities required to induce depletion of Ca2+ from intracellular stores and provide insights into the ability of T cells to tailor the use of the CD8 co-receptor to enhance Ca2+ release from the ER. This in turn modulates Ca2+ influx from the extracellular environment, ultimately controlling T cell activation. PMID:20053942

  17. "Even a highwayman, a house-breaker or a murderer" : International compassion as an insufficient universal / Jim Hicks

    Index Scriptorium Estoniae

    Hicks, Jim, 1959-

    2000-01-01

    Bosnia sõja reportaažide kirjandusteaduslik analüüs. Vaadeldud on ameerika ajakirjanike raamatuid: Kaplan, Robert. Balkan ghosts (Balkani vaimud) ; Sudetic, Chuck. Blood and vengeance (Veri ja kättemaks)

  18. "Even a highwayman, a house-breaker or a murderer" : International compassion as an insufficient universal / Jim Hicks

    Index Scriptorium Estoniae

    Hicks, Jim, 1959-

    2000-01-01

    Bosnia sõja reportaažide kirjandusteaduslik analüüs. Vaadeldud on ameerika ajakirjanike raamatuid: Kaplan, Robert. Balkan ghosts (Balkani vaimud) ; Sudetic, Chuck. Blood and vengeance (Veri ja kättemaks)

  19. Experimental particle physics at the University of Pittsburgh. [Dept. of Physics and Astronomy

    Energy Technology Data Exchange (ETDEWEB)

    Cleland, W.E.; Engels, E. Jr.; Humanic, T.J.; Perera, U.; Shepard, P.F.; Thompson, J.A.

    1993-04-01

    During the past year on Task A, the HELIOS work drew to a close with first results from the electron-muon pair studies (no anomalous sources are seen, and the final results and uncertainties are being set). First data from CMD2 will allow improvement of some phi branching ratios, including some improved limits on forbidden decays. The engineering run for E865 is scheduled for June and July of 1993. The principal efforts of Task B, the Fermilab program, have been the completion of the analysis of the 1987--88 data with resulting publications, completion of the 1990--91 data run, and the beginning of the analysis of the 1990--91 data. In addition, the Task B group is taking a leadership role in developing a proposal to Fermilab for the upgrade of the CDF silicon vertex detector in preparation for the 1995 data run. Task C has recently submitted results of its fractionally charged particle searches, placing new upper limits on the abundance of naturally-occurring fractionally-charged particles in various materials. This group has recently been approved by the Brookhaven management for an exposure of their p-i-n diodes in a high intensity proton beam. This measurement, along with its subsequent analysis, will complete the program. Task D concerned itself with silicon drift chamber studies for the SSC. Task E was devoted to studies of electronics for the GEM Level 1 liquid Ar calorimeter trigger.

  20. Presentation to Ohio State University Dept. of Electrical Engineering ElectroScience Laboratory

    Science.gov (United States)

    Fujikawa, Gene

    2002-01-01

    Presentation made during visit to The Ohio State University, ElectroScience Laboratory, on November 14, 2002. An overview of NASA and selected technology products from the Digital Communications Technology Branch (5650) for fiscal year 2003 are highlighted. The purpose of the meeting was to exchange technical information on current aeronautics and space communications research and technology being conducted at NASA Glenn Research Center and to promote faculty/student collaborations of mutual interest.

  1. Studies in Medium Energy Physics. [Dept. of Physics, Univ. of Texas at Austin, Austin, Texas

    Energy Technology Data Exchange (ETDEWEB)

    Hoffmann, G.W.; McDonough, J.; Purcell, M.J.; Ray, R.L.; Read, D.M.; Worm, S.D.

    1992-12-01

    Progress is briefly reported in the following areas: p + A precision elastic forward-angle cross sections for 500- to 800-MeV p on [sup 40]Ca; precision measurement of D[sub NN] for [sup 13]C([rvec p], [rvec p]) at 500 MeV; design of a polarized nuclear target; search for very rare K[sub L] decays; search for the H dibaryon; experimental search for quark -- gluon plasma; and theoretical work on proton -- nucleus scattering.

  2. Research in elementary particle physics. [Dept. of Physics and Astronomy, Louisiana State Univ,. Baton Rouge, Louisiana

    Energy Technology Data Exchange (ETDEWEB)

    Chan, L.H; Haymaker, R.; Imlay, R.; McNeil, R.; Metcalf, W.; Svoboda, R.

    1992-01-01

    Theoretical work on effective action expansion on an effective low; energy theory of hadron, dynamical symmetry breaking, and lattice gauge theories is described. The high-energy experimental group at Louisiana State University has analyzed data on a neutrino oscillation experiment at LAMPF. Preparations for the LSND neutrino experiment have stated. IMB data have also been analyzed. On the ZEUS electron n-proton colliding bean experiment, the production of the barrel calorimeter has been completed. Several modules of the calorimeter have been tested at Fermilab, and preparations for data taking are underway.

  3. Pharmacy Locations, Pharmacies, licensed - name, address, contact info, Published in 2006, Iowa Dept. of Public Health.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Pharmacy Locations dataset, was produced all or in part from Published Reports/Deeds information as of 2006. It is described as 'Pharmacies, licensed - name,...

  4. Solvent-extractable lipids in an acid andic forest soil; variations with dept and season

    NARCIS (Netherlands)

    Naafs, Derck Ferdinand Werner; Bergen, P.F. van; Boogert, S.J.; Leeuw, J.W. de

    2004-01-01

    Total lipid extracts from an acid andic soil profile located on Madeira Island (Portugal) were analysed using gas chromatography (GC) and GC–mass spectrometry (GC/MS). The profile was covered mainly by grass. Bulk soil characteristics determined included soil pH (H2O) ranging from 4.5 to 4.0 and TOC

  5. 2010 King County (WA) Dept of Natural Resources and Parks Lidar: Cedar River

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Watershed Sciences, Inc. (WS) collected Light Detection and Ranging (LiDAR) data and true-color orthophotographs of King County, WA from March 18 to March 20, 2010....

  6. Hurricane and Tsunami Inundation Areas, Evacuation Zones, Published in unknown, Manatee County Information Services Dept..

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Hurricane and Tsunami Inundation Areas dataset, was produced all or in part from Other information as of unknown. It is described as 'Evacuation Zones'. Data...

  7. Docks and Piers, Fishing Piers in, Published in unknown, Manatee County Information Services Dept..

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Docks and Piers dataset as of unknown. It is described as 'Fishing Piers in'. Data by this publisher are often provided in State Plane coordinate system; in a...

  8. Elementary particle interactions. [Dept. of Physics and Astronomy, Univ. of Tennessee, Knoxville, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    Bugg, W.M.; Condo, G.T.; Handler, T.; Hart, E.L.; Read, K.; Ward, B.F.L.

    1992-10-01

    Work continues on strange particle production in weak interactions using data from a high-energy neutrino exposure in a freon bubble chamber. Meson photoproduction has also consumed considerable effort. Detector research and development activities have been carried out.

  9. Coastal Barrier Resource Areas, CBRA, Published in unknown, Manatee County Information Services Dept..

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Coastal Barrier Resource Areas dataset, was produced all or in part from Other information as of unknown. It is described as 'CBRA'. Data by this publisher are...

  10. Semiempirical studies of atomic structure. [Dept. of Physics and Astronomy, Univ. of Toledo

    Energy Technology Data Exchange (ETDEWEB)

    Curtis, L.J.

    1992-01-01

    The energy level structure, transition probabilities, and general spectroscopic properties of highly ionized many-electron systems are studied through the combined use of sensitive semiempirical data systematizations, selected precision experimental measurements, and specialized theoretical computations. Measurements are made primarily through the use of fast ion beam excitation methods, which are combined with available data from laser-and tokamak-produced plasmas, astrophysical sources, and conventional light sources. The experimental studies are strengthened through large-scale ab initio calculations. Typical examples are the following: lifetime measurements in the neon isoelectronic sequence; multiplexed decay curve measurements of Li-like Si XII; and isoelectronic specification of intershell resonance and intercombination decay rates using measured transition probabilities and spectroscopically determined singlet-mixing amplitudes.

  11. U.S. Dept. Veterans Affairs (VA) SMEC-bio Reporting for Leadership Decision Support

    OpenAIRE

    2013-01-01

    Objective To assess Reports sent from the United States VA Subject Matter Expertise Center for Biological Events (SMEC-bio) – a proof-of-concept decision support initiative – to the VA Integrated Operations Center (VA IOC). Introduction VA is the U.S. federal agency responsible for providing services to America’s Veterans. Within VA, VHA is the organization responsible for administration of health care services. VHA, with 152 Medical Centers and over 900 outpatient clinics located throughout ...

  12. Bartuccelli, M. V.; Woolcock, C. J. (University of Surrey, Dept. of Mathematics, Guildford (GB))

    Energy Technology Data Exchange (ETDEWEB)

    On the positivity of solutions for a generalized diffusion model

    2001-12-01

    The objective of this paper aims to prove positivity of solutions for the following semilinear dissipative partial differential equation: u{sub t} -{alpha}{upsilon}{sub x}xxx - {upsilon}{sub x}x - {upsilon}{upsilon}{sub x} + {upsilon}(1-{upsilon}{sup 2}). The equation can be considered as a model equation representing a class of dissipative partial differential equations containing differential operators of higher order than the Laplacian. It appears in various scientific contexts including population dynamics and chemical reactions, where the solutions of the model must be positive functions.

  13. Provenance of the Upper Triassic siliciclastics of the Mecsek Mountains and Villány Hills (Pannonian Basin, Hungary): constraints to the Early Mesozoic paleogeography of the Tisza Megaunit

    Science.gov (United States)

    Pozsgai, Emília; Józsa, Sándor; Dunkl, István; Sebe, Krisztina; Thamó-Bozsó, Edit; Sajó, István; Dezső, József; von Eynatten, Hilmar

    2016-10-01

    The Tisza Megaunit in the Southern Pannonian Basin formed part of the southern margin of the European Plate in the Early Mesozoic era. Its exact paleo-position and relation to other structural blocks is disputed for a long time. Detrital zircon U-Pb dating, heavy mineral analysis and petrographical examination of Carnian to Pliensbachian sandstone members lead to better understanding of the provenance of clastic deposits after the Ladinian-Carnian carbonate to siliciclastic facies shift in the Southwestern Tisza Megaunit. Investigations allow for constraining its paleogeographic relation to adjacent units. The Carnian and Pliensbachian siliciclastics of the Villány Hills derive from inside the Southwestern Tisza Megaunit, i.e. the medium-grade polymetamorphic rocks of the adjacent Slavonian Mountains or similar basement fragments. The Upper Triassic clastic deposits of the Mecsek Mountains most likely derive from Variscan felsic plutonic rocks of the local basement or partially from the Southern/Southwestern Bohemian Massif. About 200 Ma zircon U-Pb ages are tentatively interpreted as traces of synsedimentary distal volcanism in the Central Atlantic Magmatic Province.

  14. NY-ESO-1-Specific Circulating CD4+ T Cells in Ovarian Cancer Patients Are Prevalently TH1 Type Cells Undetectable in the CD25+FOXP3+Treg Compartment

    Science.gov (United States)

    Redjimi, Nassima; Duperrier-Amouriaux, Karine; Raimbaud, Isabelle; Luescher, Immanuel; Dojcinovic, Danijel; Classe, Jean-Marc; Berton-Rigaud, Dominique; Frenel, Jean-Sébastien; Bourbouloux, Emmanuelle

    2011-01-01

    Spontaneous CD4+ T-cell responses to the tumor-specific antigen NY-ESO-1 (ESO) are frequently found in patients with epithelial ovarian cancer (EOC). If these responses are of effector or/and Treg type, however, has remained unclear. Here, we have used functional approaches together with recently developed MHC class II/ESO tetramers to assess the frequency, phenotype and function of ESO-specific cells in circulating lymphocytes from EOC patients. We found that circulating ESO-specific CD4+ T cells in EOC patients with spontaneous immune responses to the antigen are prevalently TH1 type cells secreting IFN-γ but no IL-17 or IL-10 and are not suppressive. We detected tetramer+ cells ex vivo, at an average frequency of 1∶25000 memory cells, that is, significantly lower than in patients immunized with an ESO vaccine. ESO tetramer+ cells were mostly effector memory cells at advanced stages of differentiation and were not detected in circulating CD25+FOXP3+Treg. Thus, spontaneous CD4+ T-cell responses to ESO in cancer patients are prevalently of TH1 type and not Treg. Their relatively low frequency and advanced differentiation stage, however, may limit their efficacy, that may be boosted by immunogenic ESO vaccines. PMID:21829534

  15. NY-ESO-1-specific circulating CD4+ T cells in ovarian cancer patients are prevalently T(H)1 type cells undetectable in the CD25+ FOXP3+ Treg compartment.

    Science.gov (United States)

    Redjimi, Nassima; Duperrier-Amouriaux, Karine; Raimbaud, Isabelle; Luescher, Immanuel; Dojcinovic, Danijel; Classe, Jean-Marc; Berton-Rigaud, Dominique; Frenel, Jean-Sébastien; Bourbouloux, Emmanuelle; Valmori, Danila; Ayyoub, Maha

    2011-01-01

    Spontaneous CD4(+) T-cell responses to the tumor-specific antigen NY-ESO-1 (ESO) are frequently found in patients with epithelial ovarian cancer (EOC). If these responses are of effector or/and Treg type, however, has remained unclear. Here, we have used functional approaches together with recently developed MHC class II/ESO tetramers to assess the frequency, phenotype and function of ESO-specific cells in circulating lymphocytes from EOC patients. We found that circulating ESO-specific CD4(+) T cells in EOC patients with spontaneous immune responses to the antigen are prevalently T(H)1 type cells secreting IFN-γ but no IL-17 or IL-10 and are not suppressive. We detected tetramer(+) cells ex vivo, at an average frequency of 1:25,000 memory cells, that is, significantly lower than in patients immunized with an ESO vaccine. ESO tetramer(+) cells were mostly effector memory cells at advanced stages of differentiation and were not detected in circulating CD25(+)FOXP3(+)Treg. Thus, spontaneous CD4(+) T-cell responses to ESO in cancer patients are prevalently of T(H)1 type and not Treg. Their relatively low frequency and advanced differentiation stage, however, may limit their efficacy, that may be boosted by immunogenic ESO vaccines.

  16. Assessment of the Technology and Practice for Determining Casing Degradation during Offshore Drilling Operations.

    Science.gov (United States)

    1982-09-01

    Ecologic Instruments Bohemia NY Envirotech 12881 Knott Ave. Ste 106 Ecosystem Research and Garden Grove, CA 92645 Technology Corp. P. 0. Box 35712...Environmental Olympus Corp. of America/ Instruments, Inc. Indusria fib ericDe P. 0. Box 590 Industrial Fiberoptics Dept. Pilgrim Station 2 Nevada Drive

  17. Condensed-Phase Processes during Solid Propellant Combustion. 3. Preliminary Depth-Profiling Studies on XM39, JA2, M9, M30, and HMX2

    Science.gov (United States)

    1994-01-01

    Campus Library W.C. Strahle ATTN: K. Brezinsky B.T. Zinn I. Glassman Atlanta, GA 30332 P.O. Box 710 Princeton, NJ 08540 University of Illinois Dept. of...White Rocket Center, WV 26726 B i3. Wenzel 345 E. 47th Street 1 Hercules, Inc. New York, NY 10017 ATTN: R.V. Cartwright 100 Howard Blvd. Atlantic

  18. Generating Natural Language Under Pragmatic Constraints.

    Science.gov (United States)

    1987-03-01

    Jerome Feldman I copy Univ. of Rochester Dept. of Computer Science Rochester, NY 14627 Dr. Nils Nilsson I copy Stanford Research Irstitute Menlo Park, CA...as Cognitive Phenomena, in Contemporary Approaches to Cognition, Bruner .1. (ed), Cambridge: Harvard University Press, 1957. 148. Osgood, C.E. & Bock

  19. Terrestrial biospheric and oceanic CO2 uptakes estimated from long-term measurements of atmospheric CO2 mole fraction, δ13C, and δ(O2/N2) at Ny-Ålesund, Svalbard

    Science.gov (United States)

    Goto, Daisuke; Morimoto, Shinji; Ishidoya, Shigeyuki; Aoki, Shuji; Nakazawa, Takakiyo

    2017-05-01

    Systematic observations of CO2 mole fraction, the isotopic ratio δ13C of CO2, and oxygen to nitrogen ratio (δ(O2/N2)) in the atmosphere have been carried out at Ny-Ålesund, Svalbard, since 1991, 1996, and 2001, respectively. The CO2 mole fraction shows a clear seasonal cycle superimposed on a secular increase with an average rate of 2.0 ppm yr-1 for the period of 1996-2013. On the other hand, δ13C and δ(O2/N2) decrease secularly at an average rate of -0.020‰ yr-1 for 1996-2013 and -19.9 per meg yr-1 for 2001-2013, respectively. Based on the observed secular trends of the CO2 mole fraction and δ(O2/N2), the average CO2 uptake during 2001-2013 was estimated to be 1.6 ± 0.8 and 2.3 ± 0.5 GtC yr-1 for the terrestrial biosphere and the ocean, respectively. By using the observed CO2 and δ13C, the corresponding CO2 uptakes of 1.3 ± 0.6 and 2.6 ± 0.5 GtC yr-1 were obtained for the same period. The estimates from the two methods are in good agreement with each other. The terrestrial biospheric CO2 uptake derived by the latter method showed large interannual variability in association with El Niño events. On the other hand, the oceanic uptake increased secularly with less interannual variability during 1996-2013.

  20. Enhanced thermal stability and mechanical properties of nitrogen deficient titanium aluminum nitride (Ti0.54Al0.46Ny) thin films by tuning the applied negative bias voltage

    Science.gov (United States)

    Calamba, K. M.; Schramm, I. C.; Johansson Jõesaar, M. P.; Ghanbaja, J.; Pierson, J. F.; Mücklich, F.; Odén, M.

    2017-08-01

    Aspects on the phase stability and mechanical properties of nitrogen deficient (Ti0.54Al0.46)Ny alloys were investigated. Solid solution alloys of (Ti,Al)N were grown by cathodic arc deposition. The kinetic energy of the impinging ions was altered by varying the substrate bias voltage from -30 V to -80 V. Films deposited with a high bias value of -80 V showed larger lattice parameter, finer columnar structure, and higher compressive residual stress resulting in higher hardness than films biased at -30 V when comparing their as-deposited states. At elevated temperatures, the presence of nitrogen vacancies and point defects (anti-sites and self-interstitials generated by the ion-bombardment during coating deposition) in (Ti0.54Al0.46)N0.87 influence the driving force for phase separation. Highly biased nitrogen deficient films have point defects with higher stability during annealing, which cause a delay of the release of the stored lattice strain energy and then accelerates the decomposition tendencies to thermodynamically stable c-TiN and w-AlN. Low biased nitrogen deficient films have retarded phase transformation to w-AlN, which results in the prolongment of age hardening effect up to 1100 °C, i.e., the highest reported temperature for Ti-Al-N material system. Our study points out the role of vacancies and point defects in engineering thin films with enhanced thermal stability and mechanical properties for high temperature hard coating applications.