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Sample records for hibiscus sabdariffa calyces

  1. Quercetin, kaempferol, myricetin, and fatty acid content among several Hibiscus sabdariffa accession calyces based on maturity in a greenhouse

    Science.gov (United States)

    Flavonols including quercetin, kaempferol, myricetin, and fatty acids in plants have many useful health attributes including antioxidants, cholesterol lowering, and cancer prevention. Six accessions of roselle, Hibiscus sabdariffa calyces were evaluated for quercetin, kaempferol, and myricetin conte...

  2. Antibacterial activity of Hibiscus sabdariffa L. calyces against hospital isolates of multidrug resistant Acinetobacter baumannii

    Institute of Scientific and Technical Information of China (English)

    Emad Mohamed Abdallah

    2016-01-01

    Objective: To evaluate the antibacterial activity of methanol extract of Hibiscus sab-dariffa (H. sabdariffa) calyces employed in Sudanese folk medicine against five hospital isolates of multidrug resistant Acinetobacter baumannii (MDR A. baumannii). Methods: The antibacterial activity of 80% methanol extract (v/v) of H. sabdariffa calyces was evaluated by agar disc diffusion, minimum inhibitory concentration and minimum bactericidal concentration methods. Antibiotic susceptibility of selected A. baumannii strains was tested. Results: In the present investigation, the methanol extract from the calyces of H. sabdariffa exhibited significant antibacterial properties against the non-MDR A. baumannii as well as the MDR A. baumannii strains with a zone of inhibition ranging from (11.3 ± 0.3) to (13.6 ± 0.3) mm. The relative percentage inhibition of H. sabdariffa extract (10 mg/disc) with respect to gentamicin (10 mg/disc) had potent antibacterial properties and was much more effective than gentamicin. Values of mini-mum inhibitory concentration and minimum bactericidal concentration ranged from 25 to 50 and 50 to 100 mg/mL, respectively, revealing the potential bactericidal properties of the extract. Conclusions: According to the present study, the calyces of H. sabdariffa can be used as a substitute source of the current ineffective synthetic antibiotics used against MDR A. baumannii.

  3. Antibacterial activity of Hibiscus sabdariffa L. calyces against hospital isolates of multidrug resistant Acinetobacter baumannii

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    Emad Mohamed Abdallah

    2016-11-01

    Full Text Available Objective: To evaluate the antibacterial activity of methanol extract of Hibiscus sabdariffa (H. sabdariffa calyces employed in Sudanese folk medicine against five hospital isolates of multidrug resistant Acinetobacter baumannii (MDR A. baumannii. Methods: The antibacterial activity of 80% methanol extract (v/v of H. sabdariffa calyces was evaluated by agar disc diffusion, minimum inhibitory concentration and minimum bactericidal concentration methods. Antibiotic susceptibility of selected A. baumannii strains was tested. Results: In the present investigation, the methanol extract from the calyces of H. sabdariffa exhibited significant antibacterial properties against the non-MDR A. baumannii as well as the MDR A. baumannii strains with a zone of inhibition ranging from (11.3 ± 0.3 to (13.6 ± 0.3 mm. The relative percentage inhibition of H. sabdariffa extract (10 mg/disc with respect to gentamicin (10 mg/disc had potent antibacterial properties and was much more effective than gentamicin. Values of minimum inhibitory concentration and minimum bactericidal concentration ranged from 25 to 50 and 50 to 100 mg/mL, respectively, revealing the potential bactericidal properties of the extract. Conclusions: According to the present study, the calyces of H. sabdariffa can be used as a substitute source of the current ineffective synthetic antibiotics used against MDR A. baumannii.

  4. Aqueous Extracts of Hibiscus sabdariffa Calyces Decrease Hepatitis A Virus and Human Norovirus Surrogate Titers.

    Science.gov (United States)

    Joshi, Snehal S; Dice, Lezlee; D'Souza, Doris H

    2015-12-01

    Hibiscus sabdariffa extract is known to have antioxidant, anti-diabetic, and antimicrobial properties. However, their effects against foodborne viruses are currently unknown. The objective of this study was to determine the antiviral effects of aqueous extracts of H. sabdariffa against human norovirus surrogates (feline calicivirus (FCV-F9) and murine norovirus (MNV-1)) and hepatitis A virus (HAV) at 37 °C over 24 h. Individual viruses (~5 log PFU/ml) were incubated with 40 or 100 mg/ml of aqueous hibiscus extract (HE; pH 3.6), protocatechuic acid (PCA; 3 or 6 mg/ml, pH 3.6), ferulic acid (FA; 0.5 or 1 mg/ml; pH 4.0), malic acid (10 mM; pH 3.0), or phosphate buffered saline (pH 7.2 as control) at 37 °C over 24 h. Each treatment was replicated thrice and plaque assayed in duplicate. FCV-F9 titers were reduced to undetectable levels after 15 min with both 40 and 100 mg/ml HE. MNV-1 was reduced by 1.77 ± 0.10 and 1.88 ± 0.12 log PFU/ml after 6 h with 40 and 100 mg/ml HE, respectively, and to undetectable levels after 24 h by both concentrations. HAV was reduced to undetectable levels by both HE concentrations after 24 h. PCA at 3 mg/ml reduced FCV-F9 titers to undetectable levels after 6 h, MNV-1 by 0.53 ± 0.01 log PFU/ml after 6 h, and caused no significant change in HAV titers. FA reduced FCV-F9 to undetectable levels after 3 h and MNV-1 and HAV after 24 h. Transmission electron microscopy showed no conclusive results. The findings suggest that H. sabdariffa extracts have potential to prevent foodborne viral transmission.

  5. Wound healing potential of formulated extract from hibiscus sabdariffa calyx.

    Science.gov (United States)

    Builders, P F; Kabele-Toge, B; Builders, M; Chindo, B A; Anwunobi, Patricia A; Isimi, Yetunde C

    2013-01-01

    Wound healing agents support the natural healing process, reduce trauma and likelihood of secondary infections and hasten wound closure. The wound healing activities of water in oil cream of the methanol extract of Hibiscus sabdariffa L. (Malvaceae) was evaluated in rats with superficial skin excision wounds. Antibacterial activities against Pseudomonas aeroginosa, Staphylococcus aureus and Echerichia coli were determined. The total flavonoid content, antioxidant properties and thin layer chromatographic fingerprints of the extract were also evaluated. The extract demonstrated antioxidant properties with a total flavonoid content of 12.30±0.09 mg/g. Six reproducible spots were obtained using methanol:water (95:5) as the mobile phase. The extract showed no antimicrobial activity on the selected microorganisms, which are known to infect and retard wound healing. Creams containing H. sabdariffa extract showed significant (Pextract. This study, thus, provides evidence of the wound healing potentials of the formulated extract of the calyces of H. sabdariffa and synergism when co-formulated with gentamicin.

  6. Hibiscus sabdariffa L. tea (tisane) lowers blood pressure in prehypertensive and mildly hypertensive adults: a randomized, placebo-controlled clinical trial

    Science.gov (United States)

    Background: In vitro studies have shown Hibiscus sabdariffa L., an ingredient found in many herbal tea blends and other beverages, has antioxidant properties, and, in animal models, extracts of its calyces have demonstrated hypocholesterolemic and anti-hypertensive properties. Objective: To exa...

  7. In vitro vasorelaxation mechanisms of bioactive compounds extracted from Hibiscus sabdariffa on rat thoracic aorta

    Science.gov (United States)

    Sarr, Mamadou; Ngom, Saliou; Kane, Modou O; Wele, Alassane; Diop, Doudou; Sarr, Bocar; Gueye, Lamine; Andriantsitohaina, Ramaroson; Diallo, Aminata S

    2009-01-01

    Background In this study, we suggested characterizing the vasodilator effects and the phytochemical characteristics of a plant with food usage also used in traditional treatment of arterial high blood pressure in Senegal. Methods Vascular effects of crude extract of dried and powdered calyces of Hibiscus sabdariffa were evaluated on isolated thoracic aorta of male Wistar rats on organ chambers. The crude extract was also enriched by liquid-liquid extraction. The various cyclohexane, dichloromethane, ethyl acetate, butanol extracts obtained as well as the residual marc were subjected to Sephadex LH-20 column chromatography. The different methanolic eluate fractions were then analyzed by Thin Layer (TLC) and High Performance Liquid Chromatography (HPLC) and their vascular effects also evaluated. Results The H. Sabdariffa crude extract induced mainly endothelium-dependent relaxant effects. The endothelium-dependent relaxations result from NOS activation and those who not dependent to endothelium from activation of smooth muscle potassium channels. The phytochemical analysis revealed the presence of phenolic acids in the ethyl acetate extract and anthocyans in the butanolic extract. The biological efficiency of the various studied extracts, in term of vasorelaxant capacity, showed that: Butanol extract > Crude extract > Residual marc > Ethyl acetate extract. These results suggest that the strong activity of the butanolic extract is essentially due to the presence of anthocyans found in its fractions 43-67. Conclusion These results demonstrate the vasodilator potential of hibiscus sabdariffa and contribute to his valuation as therapeutic alternative. PMID:19883513

  8. Thermal stability of anthocyanin extract of Hibiscus sabdariffa L. in the presence of beta-cyclodextrin.

    Science.gov (United States)

    Mourtzinos, Ioannis; Makris, Dimitris P; Yannakopoulou, Konstantina; Kalogeropoulos, Nick; Michali, Iliana; Karathanos, Vaios T

    2008-11-12

    The thermal stability of anthocyanin extract isolated from the dry calyces of Hibiscus sabdariffa L. was studied over the temperature range 60-90 degrees C in aqueous solutions in the presence or absence of beta-cyclodextrin (beta-CD). The results indicated that the thermal degradation of anthocyanins followed first-order reaction kinetics. The temperature-dependent degradation was adequately modeled by the Arrhenius equation, and the activation energy for the degradation of H. sabdariffa L. anthocyanins during heating was found to be approximately 54 kJ/mol. In the presence of beta-CD, anthocyanins degraded at a decreased rate, evidently due to their complexation with beta-CD, having the same activation energy. The formation of complexes in solution was confirmed by nuclear magnetic resonance studies of beta-CD solutions in the presence of the extract. Moreover, differential scanning calorimetry revealed that the inclusion complex of H. sabdariffa L. extract with beta-CD in the solid state was more stable against oxidation as compared to the free extract, as the complex remained intact at temperatures 100-250 degrees C where the free extract was oxidized. The results obtained clearly indicated that the presence of beta-CD improved the thermal stability of nutraceutical antioxidants present in H. sabdariffa L. extract, both in solution and in solid state.

  9. Chemistry and quality of Hibiscus (Hibiscus sabdariffa) for developing the natural-product industry in Senegal.

    Science.gov (United States)

    Juliani, H R; Welch, C R; Wu, Q; Diouf, B; Malainy, D; Simon, J E

    2009-03-01

    The objectives of this study were to assess and improve the quality of the hibiscus calyces from Senegal over 2 production seasons (2004 to 2005), to develop and adapt new procedures for the determination of hibiscus anthocyanins and analysis of the 2 major ones, delphinidin-3-sambubioside and cyanidin-3-sambubioside. The foreign matter, total ashes, and acid insoluble ashes showed that the calyces harvested in 2005 were produced following hygienic practices, while the color assessment of the calyces and analysis of hibiscus active principles also showed higher amounts of anthocyanins in 2005. A protocol to measure anthocyanins by pH-differential UV-Vis spectrophotometry was adapted to measure the hibiscus anthocyanins from a water extract. The spectrophotometric method for quantitation of total anthocyanins showed a close correlation (r(2)= 0.82) when compared with the HPLC method, suggesting the use of the colorimetric method in quality control programs as an affordable alternative method to assess anthocyanin content in hibiscus. New and raised standards for the cleanliness and active principle content in hibiscus are also proposed. This study demonstrated that the implementation of a quality control program and the application of agricultural good practices in the production and processing of hibiscus calyces can lead to higher quality natural plant products.

  10. Morphological characteristics and pathogenicity of fungi associated with Roselle (Hibiscus Sabdariffa) diseases in Penang, Malaysia.

    Science.gov (United States)

    Eslaminejad, Touba; Zakaria, Maziah

    2011-11-01

    Roselle, or Jamaica sorrel (Hibiscus sabdariffa) is a popular vegetable in many tropical regions, cultivated for its leaves, seeds, stems and calyces which, the dried calyces are used to prepare tea, syrup, jams and jellies and as beverages. The main objectives of this study were to identify and characterise fungal pathogens associated with Roselle diseases based on their morphological and cultural characteristics and to determine the pathogenicity of four fungi infecting Roselle seedlings, namely Phoma exigua, Fusarium nygamai, Fusarium tgcq and Rhizoctonia solani in Penang. A total of 200 fungal isolates were obtained from 90 samples of symptomatic Roselle tissues. The isolates were identified based on cultural and morphological characteristics, as well as their pathogenicity. The fungal pathogen most frequently isolated was P. exigua (present in 45% of the samples), followed by F. nygamai (25%), Rhizoctonia solani (19%) and F. camptoceras (11%). Pathogenicity tests showed that P. exigua, F. nygamai, F. camptoceras and R. solani were able to infect both wounded and unwounded seedlings with different degrees of severity as indicated by the Disease severity (DS). R. solani was the most pathogenic fungus affecting both wounded and unwounded Roselle seedlings, followed by P. exigua that was highly pathogenic on wounded seedlings. F. nygamai was less pathogenic while the least pathogenic fungus was F. camptoceras, infecting only the unwounded seedlings but, surprisingly, not the wounded plants.

  11. Phenolic compounds of Hibiscus sabdariffa and influence of organic residues on its antioxidant and antitumoral properties

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    ASN. Formagio

    Full Text Available The aim of this study was to evaluate the phenolic and flavonoids contents and the antioxidant and antitumoral activity of leaf and calyx methanolic extracts from Hibiscus sabdariffa (roselle cultivated with poultry litter and organosuper® under three modes of application. The total phenolic content in the each extract was determined using the Folin-Ciocalteu reagent and for aluminium chloride flavonoids. The antioxidant parameters were analyzed using a 2, 2-diphenyl-1-picrylhydrazyl (DPPH. free radical scavenging assay. An antitumor colorimetric assay using sulforhodamine B. The highest contents of phenolic and flavonoids were observed in leaf extracts (389.98 and 104.52 mg g–1, respectively and calyx extracts (474.09 and 148.35 mg g–1, respectively from plants cultivated with organosuper®, although these values did not differ significantly from those observed for the other treatments. The average IC50 of leaves (43.48 μg mL–1 and calyces (37.15 μg mL–1 demonstrated that both have substances that may contribute to free radical scavenging action. The methanol extract from calyces showed significant selective activity against a leukemia line (K-562, with IC50 values of 0.12 mg mL–1 (organosuper® and 1.16 mg mL–1 (poultry litter, with concentration-dependent, cytotoxic and cytocidal effects.

  12. Lactogenic study of the ethyl-acetate fraction of Hibiscus sabdariffa linn seed on pituitary prolactin level of lactating albino rats

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    I G Bako

    2013-03-01

    Full Text Available Summary. The effect of ethyl-acetate fraction of Hibiscus sabdariffa l. seed on pituitary prolactin and milk production was evaluated in albino rats. Twenty four lactating rats were randomly grouped at parturition into control, metoclopramide-treated and Ethyl-acetate-treated group consist of six rats in each group (n=6. The lactating rats were administered control (normal saline, metoclopramide (5mg/kg and ethyl-acetate fraction (100 and 200mg/kg respectively from day 3-17 of lactation. Milk yield for rats were estimated by pup weight and weight gain. The animals were then euthanized on the day 18 and pituitary prolactin was analyzed using prolactin kit. The prolactin level of ethyl-acetate fraction of Hibiscus sabdariffa showed a significant increase (P<0.01 when compared to control group. Pup weight gain was also significantly higher (P<0.05 than the control group. This can be inferred that ethyl-acetate fraction of Hibiscus sabdariffa l. seed has lactogenic activity because it increases pituitary prolactin level and milk production in lactating female albino rats. The LD50 of Ethyl-acetate fraction Hibiscus sabdariffa l. was found to be above 5000mg/kg. Industrial relevance. The outstanding advantage of this galactagogue option is that, it is safer, affordable and tolerable, and it is taken as an alternative in preference to anti-psychotic drugs that have side effect of drowsiness and depression. The plant calyces, leaves and seeds are eaten as foods because it contain substantial amount of essential fatty, Tocopherol (Vitamin E, ascorbic acid (Vitamin C, mineral salts calcium, magnesium, sodium, potassium and phosphorus. Keywords. Hibiscus sabdariffa; prolactin; lactation; milk; pituitary

  13. Hibiscus sabdariffa extractivities on cadmium-mediated alterations of human U937 cell viability and activation

    Institute of Scientific and Technical Information of China (English)

    Tebekeme Okoko; Diepreye Ere

    2012-01-01

    Objective:To investigate the effect of the anthocyanin-rich extract of Hibiscus sabdariffa (H. sabdariffa) calyx on the viability of cadmium-treated U937 cells and cadmium-mediated activation of U937-derived macrophages. Methods:The macrophage cell line U937 was treated with cadmium (0.1μmol/L) and later incubated with the anthocyanin-rich extract and cell viability was assessed via trypan blue staining. In the other experiment, the U937 cells were transformed to the macrophage form by treatment with phorbol 12, myristate 13, and acetate and incubated with cadmium (10μmol/L). The anthocyanin-rich extract was added to the cells later and subsequently, the supernatant of each cell culture was analysed for the production of tumour necrosis factor-alpha (TNF-α), interleukin 1 (IL-1), interleukin 6 (IL-6), nitric oxide, and catalase activity as indices for the activation of macrophages. Results:It revealed that the anthocynanin-rich extract significantly (P <0.05) increased the viability of the cells which was suppressed by cadmium when compared to quercetin dihydrate. The extract also reduced the cadmium-mediated production of the markers of macrophage-activation when compared to quercetin dihydrate. In both experiments, the activity of the extract was concentration-dependent (P <0.05). Conclusion:The findings show that H. sabdariffa possesses significant immunoprotective effect. These corroborate the immense reported antioxidant and medicinal potential of the calyces of the plant which could be exploited for pharmacological and neutraceutical advantages.

  14. Protocols for Callus and Somatic Embryo Initiation for Hibiscus sabdariffa L. (Malvaceae): Influence of Explant Type, Sugar, and Plant Growth Regulators

    Science.gov (United States)

    A significant work on callus induction and somatic embryogenesis was realized for Hibiscus sabdariffa. Two genotypes (Hibiscus sabdariffa and Hibiscus sabdariffa var. altissima) two sugars (sucrose and glucose) and three concentrations (1 %, 2%, 3%) of each sugar, 3 explant types (root, hypocotyl, c...

  15. Effect of the temperature on the spray drying of Roselle extracts (Hibiscus sabdariffa L.).

    Science.gov (United States)

    Gonzalez-Palomares, Salvador; Estarrón-Espinosa, Mirna; Gómez-Leyva, Juan Florencio; Andrade-González, Isaac

    2009-03-01

    The effect of the drying temperature on the volatile components and sensory acceptance of the Roselle (Hibiscus sabdariffa) extract in powder was investigated. The Roselle extraction was carried out by maceration with 7 L of 30% ethanol (v/v), 560 g of fresh Roselle calyces for 168 h. The Roselle extracts were spray dried at different temperatures 150, 160, 170, 180, 190, 200 and 210 degrees C, giving different outlet values about yield and final moisture. The volatile compounds in Roselle extract and dried samples were performed using needles of solid phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS HP-5890). Twenty volatile compounds were identified in the extracts among them terpenoids, esters, hydrocarbons and aldehydes. Fourteen volatile compounds were identified in the powder sample, but only ten were present in the Roselle extract. This indicates that some compounds were lost and some others were generated due to a degradation process. An acceptability sensory analysis showed that the best powder sample was the Roselle extract dehydrated using temperature between 190 degrees C and 200 degrees C (pspray drying temperature of the Roselle extracts has an effect on the volatile compounds losses.

  16. Histological and Biochemical Evaluation of the Kidney following Chronic Consumption of Hibiscus sabdariffa

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    U. U. Ukoha

    2015-01-01

    Full Text Available Hibiscus sabdariffa L. has been used traditionally as herbal medicine and has been documented to have a broad range of therapeutic effects. The present study aimed to investigate the effects of chronic administration of aqueous extract of flowers of Hibiscus sabdariffa on the histology of the kidney and some biochemical indices of renal function in male Wistar rats. Twenty (20 Wistar rats were randomly divided into four (4 groups of five rats each. The extract was administered orally in doses 200, 500, and 800 mg/kg body weight for 21 days. The kidney was harvested and processed histologically and blood samples were taken for biochemical assays. The histological results showed dose dependent pathological states and the biochemical analysis revealed a dose dependant variation in renal indices. These results suggest that chronic administration of aqueous extract of Hibiscus sabdariffa may be toxic to the kidney.

  17. INHIBITION OF HUMAN LOW-DENSITY LIPOPROTEINS OXIDATION BY Hibiscus radiatus CUV. CALYCES EXTRACT

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    Hernawan Hernawan

    2010-06-01

    Full Text Available Hibiscus radiatus Cuv calyces extracts rich in polyphenols was screened for their potential to inhibit oxidation of human low-density lipoproteins-cholesterol (LDL-C in vitro. The inhibition of LDL-C oxidation (antioxidant activity was determined by measuring the formation of conjugated dienes and thiobarbituric acid reagent substances (TBARS. LDL-C oxidation was carried out in the presence of H. radiatus Cuv calyces extract (20 and 50 μM. CuSO4 (10 μM was used as the oxidation initiator and  butylated hydroxytoluene (BHT at 50 μM was used as standard antioxidant. The protective effect of H. radiatus Cuv. calyces extract toward human low-density lipoproteins, complex lipid system was  demonstrated by significant increase lag time (> 103 min, diminished of the propagation rate (44 %, and diminution of conjugated dienes formation 59.42 % (50 μM compared to control.   Keywords: antioxidant, conjugated dienes, Hibiscus radiatus Cuv, low-density lipoproteins-cholesterol

  18. Evaluation of roselle (Hibiscus sabdariffa) leaf and pomegranate (Punica granatum) fruit rind for activity against Meloidogyne incognita

    Science.gov (United States)

    Pomegranate (Punica granatum) fruit and roselle (Hibiscus sabdariffa) leaves have been used in traditional medicine, including as anthelmintics. Methanolic extracts from these plants were investigated for activity against the southern root-knot nematode (RKN) Meloidogyne incognita. Dried, ground p...

  19. Hepatoprotective and antioxidant activities of Hibiscus sabdariffa petal extracts in Wistar rats

    OpenAIRE

    Abba P. Obouayeba; Lydie Boyvin; Gervais M. M'Boh; Sekou Diabat and eacute;; Tanoh H. Kouakou; Allico J. Djaman; Jean D. N'Guessan

    2014-01-01

    Background: Hibiscus sabdariffa is a medicinal plant rich in phytochemical compounds, which is the source of its biological properties. This study on the aqueous extract of H. sabdariffa (AEHS) was conducted to assess its hepatoprotective and antioxidant properties. Methods: It was carried out with 25 Wistar rats divided into five groups. Two groups were treated with a solution of NaCl 0.9%. One group was treated with silymarin at a dose of 25 mg/kg body weight (BW). Two other groups were ...

  20. Antimutagenic Effect of Hibiscus sabdariffa L. Aqueous Extract on Rats Treated with Monosodium Glutamate

    Science.gov (United States)

    Kerkhoff, Jacqueline; Vieira Júnior, Gerardo Magela; de Campos, Kleber Eduardo; Sugui, Marina Mariko

    2017-01-01

    Hibiscus sabdariffa L. is a plant of the Malvaceae family, commonly known as roselle. H. sabdariffa is known to contain antioxidant, cholesterol-lowering, antiobesity, insulin resistance reduction, antihypertensive, and skin cancer chemopreventive properties. This study evaluated the effects of H. sabdariffa aqueous extract against cyclophosphamide (CPA, 25 mg/Kg) induced damage to DNA in male Wistar rats by micronucleus test. Samples of H. sabdariffa calyx were obtained in the municipality of Barra do Garças, Mato Grosso, Brazil. The aqueous extract was prepared by infusion and each animal received a daily dose of 400 mg/Kg by gavage for 15 consecutive days of treatment. The presence of anthocyanins was confirmed by ferric chloride test and phenolic compounds using high-performance liquid chromatography, with emphasis on the identification of rutin. The animals were sacrificed by deepening of anaesthesia to obtain bone marrow and determination of the frequency of micronucleated polychromatic erythrocytes. The group treated with the aqueous extract of H. sabdariffa revealed a 91% reduction in micronucleus frequency when compared with the positive control group. Under the conditions tested, H. sabdariffa L. presented a protective effect to CPA-induced damage to DNA of the treated animals, and it is a potential candidate as a chemopreventive agent against carcinogenesis. PMID:28197528

  1. Fatty acid composition, anti-inflammatory and analgesic activities of Hibiscus sabdariffa Linn. seeds

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    Sumaia Awad Elkariem Ali

    2014-06-01

    Full Text Available Hibiscus (H. sabdariffa (commonly known as “Karkadeh” in Arabic is widely used in various pharmacological applications in Sudan. The present study was carried out to investigate the anti-inflammatory and analgesic activities of H. sabdariffa seed extracts using rat models. In acute anti-inflammatory models, oral administration of petroleum ether extract of H. sabdariffa seeds inhibited the hind paw edema (p<0.01 which was induced by carrageenan. The petroleum ether extract exhibited significant (p<0.01 inhibition of vascular permeability in rats induced by intraperitoneal injection of acetic acid (0.6%. In cotton pellet granuloma method, the petroleum ether extract of H. sabdariffa seed showed significant inhibition of granuloma. The extract reduced (p<0.001 abdominal constrictions which was induced by injection of acetic acid (0.7%. Analysis of seed oil of H. sabdariffa using Gas Chromatography revealed the presence of three fatty acids; these were linolelaidic acid, arachidic acid, and palmitic acid. In conclusion, H. sabdariffa seeds possess anti-inflammatory and analgesic activities in rat model.

  2. Effect of Hibiscus sabdariffa on blood pressure and electrolyte ...

    African Journals Online (AJOL)

    2015-02-27

    Feb 27, 2015 ... with two modifications. 20 g of dry calyces were weighed and ground in an electric mill ..... by membrane stabilization and stimulation of vascular. Na+-K+ ATPase ... liquid chromatography that is a more sensitive and accurate ...

  3. Toxicity of Dietary Hibiscus sabdariffa Linn (Malvaceae Seed to Wistar Rats

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    Amgad A. H. Al Bahi

    2013-12-01

    Full Text Available In Sudan Hibiscus sabdariffa seeds after fermentation are commonly known as Furundu with high quality proteins consumed as substitute to meat mainly in the Western part of the country. The raw seed, 10, 15 and 20 % of the standard diet was fed to Wister rats for periods up to 12 weeks. These doses were found to be non-toxic but not lethal to the treated rats. There was no evidence of carcinogenic effect of Hibiscus sabdariffa seed on any of the vital organs, but the main features were depression of growth and diverse pathological changes on the liver, kidneys and intestines which were correlated with highly significant (P<0.001 elevation of serum Aspartate aminotransferase (AST, Alanine aminotransferase (ALT and Alkaline phosphatase (ALP activities and bilirubin (P<0.01 concentration in addition to a significant decrease (P<0.01 in cholesterol concentration. No change was observed in the total protein, globulin and urea concentrations of the test groups and albumin concentration in Groups 1 and 2 fed on diet containing 10 and 15% H. sabdarrifa seed. These findings were accompanied by leukocytosis and significantly lower red blood cells and haemoglobin concentration. Due to the elevated Mean corpuscular volume (MCV and the normal values of the mean corpuscular Haemoglobin concentration (MCHC in all the treatment groups the toxicity of H. Sabdariffa seed to Wistar rats resulted in macrocytic normochromic type of anaemia.

  4. Protective effect of Hibiscus sabdariffa Linn. calyx extract on tetracycline induced testicular toxicity in mice

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    Nawaphat Taweebot

    2010-07-01

    Full Text Available Aqueous Hibiscus sabdariffa Linn. (Malvaceae calyx extract (HSE was evaluated for theprotective effect against testicular toxicity induced by tetracycline dose of 20 mg/100 gBW for 14 daysin mice. The extract doses of 20, 50 and 100 mg/100 gBW used in pretreatment by oral administrationfor 4 days and subsequent co-treatment with tetracycline for 14 days had the protective effectexhibiting significantly increasing quality of seminal fluid including an increase in total sperm count,percentage of mobile sperms and viable sperms when compared to the tetracycline treated group (p H. Sabdariffa. calyx extract may be used as protective agent againsttetracycline-induced reproductive toxicity in mice.

  5. Z-scan and optical limiting properties of Hibiscus sabdariffa dye

    CSIR Research Space (South Africa)

    Maaza, M

    2014-12-01

    Full Text Available to the difference between the normalized peak, and valley transmittances, Tp-v=Tp−Tv. Such a quantity is given by ΔTp-v =0.406 (1- S) 0.25 |0| [17]. Figure 4 reports such characteristic asymmetric profiles of the various Hibiscus Sabdariffa liquid solutions...). 2. C. Pradeep, S. Mathew, B. Nithyajam P. Radhakrishnan and V.P.N. Nampoori, Appl Phys A: Materials Science & Processing, DOI 10.1007/s00339-013-7814-0 (2013). 3. F. Z. Henari and A. Al-Saie, Nonlinear and Quantum Optics-Laser Physics, Vol. 16, No...

  6. Co-production of surfactin and a novel bacteriocin by Bacillus subtilis subsp. subtilis H4 isolated from bikalga, an African alkaline Hibiscus sabdariffa seed fermented condiment

    DEFF Research Database (Denmark)

    Compaore, C. S.; Nielsen, Dennis S.; Ouoba, L. I. I.;

    2013-01-01

    Bikalga is a Hibiscus sabdariffa seed fermented condiment widely consumed in Burkina Faso and neighboring countries. The fermentation is dominated by Bacillus subtilis group species. Ten B. subtilis subsp. subtilis (six isolates) and Bacillus licheniformis (four isolates) isolated from traditiona...

  7. Co-production of surfactin and a novel bacteriocin by Bacillus subtilis subsp. subtilis H4 isolated from bikalga, an African alkaline Hibiscus sabdariffa seed fermented condiment

    DEFF Research Database (Denmark)

    Compaore, C. S.; Nielsen, Dennis S.; Ouoba, L. I. I.

    2013-01-01

    Bikalga is a Hibiscus sabdariffa seed fermented condiment widely consumed in Burkina Faso and neighboring countries. The fermentation is dominated by Bacillus subtilis group species. Ten B. subtilis subsp. subtilis (six isolates) and Bacillus licheniformis (four isolates) isolated from traditiona...

  8. THE DEVELOPMENT OF CHITOSAN NANOPARTICLES FROM HIBISCUS SABDARIFFA L CALYX EXTRACT FROM INDONESIA AND THAILAND

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    Nurkhasanah*, Tedjo Yuwono, Laela Hayu Nurani, Muhammad Ikhwan Rizki and Krisana Kraisintu

    2015-05-01

    Full Text Available Rosella (Hibiscus sabdariffa L calyx has been reported to have an antioxidant activity. Antocyanidin is the major compound of rosella and has low bioavailability as an ionic form. Rosella extract is an ucceptable due to high acidity. Development of chitosan nanoparticle from Hibiscus sabdariffa extract were expected to improve the bioavailability and the acidity problem. We studied the optimum pH and composition ratios of chitosan, extract and tripolyphosphate (TPP to develop chitosan-nanoparticle of rosella extract. We used rosella calyx both from Indonesia and Thailand to compare the physical characteristic the extract and nanoparticles. Rosella was extracted using 60% ethanol and water, followed by evaporation using evaporator and dried by freezedryer. The optimum formation of chitosan-nanoparticles of rosella extract were observed including pH formation and composition ratios between extract: chitosan: TPP. The physical charactersitics of nanoparticles were observed using transmission electron microscope (TEM and particle size analyzer (PSA. We found that optimum composition of extract: chitosan : TPP ratios were 2 : 1 : 0.1, with optimum formation of chitosan in acetate buffer pH 4. The characteristic of nanoparticles were spherical shapes with particles size range 60 to 300 nm, polydispersity index around 0.3 and zeta potential 39.19 to 62.26 mV. The nanoparticles has high entrapment efficiency of flavonoid active compound of 81.98 to 83.83%.

  9. OPTIMISATION OF HIBISCUS SABDARIFFA AS A NATURAL COAGULANT TO TREAT CONGO RED IN WASTEWATER

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    MUN Y. YONG

    2016-02-01

    Full Text Available The process of coagulation is commonly practiced in water and wastewater treatment to reduce level of dissolved chemical, turbidity and so on with the usage of coagulant. Aluminium sulphate (alum is the most commonly used coagulant, however, recent studies show that residual aluminium in drinking water and sludge may induce Alzheimer’s disease and environmental issues. Natural coagulant which is environmental friendly and non-toxic is developed as an alternative to overcome these issues. In this work, Hibiscus Sabdariffa was studied as natural coagulant to treat dye wastewater containing Congo red. The seeds were extracted with different solvent such as distilled water, 0.5 M NaCl and 0.05 M NaOH to extract the coagulation agent. The working parameters were optimised using Response Surface Methodology (RSM. 0.5 M NaCl was found to have highest colour removal of 95.1 % among the solvents. In addition, Hibiscus Sabdariffa seed was found to be an effective coagulant that has 91.2 % colour removal at the optimal working condition of pH 2, 190 mg/L coagulant dosage at 400 ppm of dye concentration. It was also been identified that the performance of natural coagulant is comparable with conventional coagulant, aluminium sulphate with colour removal of 91.2 % and 92.3 % respectively.

  10. Aqueous Extract from Hibiscus sabdariffa Linnaeus Ameliorate Diabetic Nephropathy via Regulating Oxidative Status and Akt/Bad/14-3-3 γ in an Experimental Animal Model

    OpenAIRE

    Shou-Chieh Wang; Shiow-Fen Lee; Chau-Jong Wang; Chao-Hsin Lee; Wen-Chin Lee; Huei-Jane Lee

    2011-01-01

    Several studies point out that oxidative stress maybe a major culprit in diabetic nephropathy. Aqueous extract of Hibiscus sabdariffa L. (HSE) has been demonstrated as having beneficial effects on anti-oxidation and lipid-lowering in experimental studies. This study aimed at investigating the effects of Hibiscus sabdariffa L. on diabetic nephropathy in streptozotocin induced type 1 diabetic rats. Our results show that HSE is capable of reducing lipid peroxidation, increasing catalase and glut...

  11. Antimicrobial activity of Hibiscus sabdariffa extract against uropathogenic strains isolated from recurrent urinary tract infections

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    Issam Alshami

    2014-08-01

    Full Text Available Objective: To report the antimicrobial effect and biofilm forming capacity of the uropathogenic strains that have been isolated from recurrent urinary tract infections (UTIs in the presence of Hibiscus sabdariffa (H. sabdariffa extract. Methods: Six Escherichia coli and two Klebsiella pneumoniae isolates were collected from patients with recurrent UTIs. The susceptibility of bacterial isolates to H. sabdariffa extracts were tested by determining their minimum inhibitory concentrations (MICs, and minimum bactericidal concentration (MBC by using the broth microdilution method in accordance to Clinical and Laboratory Standards Institute guidelines. Time-kill curves were plotted against the eight isolates based on the MIC results. The biofilm forming capacity of the isolates were evaluated using the microtiter plate assay. Detection of biofilms was done using the crystal violet staining method. Results: Various levels of the extracts MIC were observed against all the uropathogenic isolates. MIC values ranged from 0.5 to 4 mg/mL, and MBC values ranged from 8 to 64 mg/mL. Both the time-kill experiment and MBC-MIC ratio demonstrated that the extracts' effect was in general, bacteriostatic. The biofilm capacity inhibition assay results showed that extracts inhibited biofilm production of all the isolates. The level of biofilm inhibition however, had varied among the bacterial strains and ranged from 8%-60% reduction in optical density. Conclusions: The results of the study support the effective potential of H. sabdariffa extract to prevent recurrent UTIs and to emphasize the significance of the plant extract, in order to approach it as a potential antimicrobial agent.

  12. STUDIES ON HIBISCUS CANNABINUS, HIBISCUS SABDARIFFA, AND CANNABINUS SATIVA PULP TO BE A SUBSTITUTE FOR SOFTWOOD PULP- PART 1: AS-AQ DELIGNIFICATION PROCESS

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    Dharm Dutt

    2010-08-01

    Full Text Available Hibiscus cannabinus, Hibiscus sabdariffa, and Cannabinus sativa, which are renewable non-woody fiber resources having characteristics similar to that of softwood (bast fibers, when used together with hardwood (core fibers, gave higher pulp yield with good mechanical strength properties when using an alkaline sulphite-anthraquinone (AS-AQ pulping process rather than a conventional kraft pulping process and bleached more readily than kraft and soda pulps with a CEHH bleaching sequence. A comparison of properties AS-AQ pulping processes with soda and kraft pulping processes of H. cannabinus, C. sativa, and H. sabdariffa was made. All the properties were found to be better than soda and kraft pulps except tear index. All of the mechanical strength properties of handsheets of AS-AQ pulp improved except tear index. Therefore, the AS-AQ pulping process can be considered as ideal for manufacturing of paper grades like greaseproof, glassine, and high-quality writing and printing paper.

  13. Hibiscus sabdariffa extract inhibits in vitro biofilm formation capacity of Candida albicans isolated from recurrent urinary tract infections

    Institute of Scientific and Technical Information of China (English)

    Issam Alshami; Ahmed E Alharbi

    2014-01-01

    Objective: To explore the prevention of recurrent candiduria using natural based approaches and to study the antimicrobial effect of Hibiscus sabdariffa (H. sabdariffa) extract and the biofilm forming capacity of Candida albicans strains in the present of the H. sabdariffa extract.Methods:In this particular study, six strains of fluconazole resistant Candida albicans isolated from recurrent candiduria were used. The susceptibility of fungal isolates, time-kill curves and biofilm forming capacity in the present of the H. sabdariffa extract were determined. Results: Various levels minimum inhibitory concentration of the extract were observed against all the isolates. Minimum inhibitory concentration values ranged from 0.5 to 2.0 mg/mL. Time-kill experiment demonstrated that the effect was fungistatic. The biofilm inhibition assay results showed that H. sabdariffa extract inhibited biofilm production of all the isolates. Conclusions: The results of the study support the potential effect of H. sabdariffa extract for preventing recurrent candiduria and emphasize the significance of the plant extract approach as a potential antifungal agent.

  14. The consumption of Hibiscus sabdariffa dried calyx ethanolic extract reduced lipid profile in rats.

    Science.gov (United States)

    Carvajal-Zarrabal, Octavio; Waliszewski, Stefan M; Barradas-Dermitz, Dulce Ma; Orta-Flores, Zaida; Hayward-Jones, Patricia M; Nolasco-Hipólito, Cirilo; Angulo-Guerrero, Ofelia; Sánchez-Ricaño, Ramón; Infanzón, Rosa M; Trujillo, Patricia R L

    2005-12-01

    The scientific basis for the statement that plants and their active constituents play an important role in the prevention of chronic and degenerative diseases is continously advancing. The object of the present study was to evaluate the effect of Hibiscus sabdariffa L. dried calyx ethanolic extract on the serum lipid profile of Sprague-Dawley rats. The rats were fed during 4 weeks with either a basal diet, containing high cholesterol (1%), cholic acid (0.25%), lard oil (10%), or a supplemental diet with H. sabdariffa extract at 5%, 10%, and 15% levels (SD(5), SD(10), SD(15)). Weight gain and faeces dry weight were both very significantly less (p hibiscus acid racemization, (+)-HCA to (-)-HCA, mediated by intestinal flora enzymes possibly explains the significant triacylglycerol decrease in all experimental groups. VLDL, the precursor of LDL, is composed predominantly of triacylglycerols, suggesting that the significant decrease in LDL is related to observed triacylglycerol synthesis inhibition. Five percent H. sabdariffa extract addition showed the best results in the reduction of serum lipids under study conditions.

  15. Variations in chemical fingerprints and major flavonoid contents from the leaves of thirty‐one accessions of Hibiscus sabdariffa L.

    Science.gov (United States)

    Wang, Jin; Cao, Xianshuang; Ferchaud, Vanessa; Jiang, Hao; Tang, Feng; Chin, Kit L.

    2015-01-01

    Abstract The leaves of Hibiscus sabdariffa L. have been used as traditional folk medicines for treating high blood pressure and fever. There are many accessions of H. sabdariffa L. throughout the world. To assess the chemical variations of 31 different accessions of H. sabdariffa L., fingerprinting analysis and quantitation of major flavonoids were performed by high‐performance liquid chromatography (HPLC). The HPLC method was validated for linearity, sensitivity, precision, repeatability and accuracy. A quadrupole‐time‐of‐flight mass spectrometry (Q‐TOF‐MS) was applied for the characterization of major compounds. A total of 9 compounds were identified, including 6 flavonoids and 3 phenolic acids. In the fingerprint analysis, similarity analysis (SA) and principal component analysis (PCA) were used to differentiate the 31 accessions of H. sabdariffa L. Based on the results of PCA and SA, the samples No. 15 and 19 appeared much different from the main group. The total content of five flavonoids varied greatly among different accessions, ranging from 3.35 to 23.30 mg/g. Rutin was found to be the dominant compound and the content of rutin could contribute to chemical variations among different accessions. This study was helpful to understand the chemical variations between different accessions of H. sabdariffa L., which could be used for quality control. © 2015 The Authors Biomedical Chromatography Published by John Wiley & Sons Ltd. PMID:26394363

  16. Antimicrobial activity of Hibiscus sabdariffa aqueous extracts against Escherichia coli O157:H7 and Staphylococcus aureus in a microbiological medium and milk of various fat concentrations.

    Science.gov (United States)

    Higginbotham, Kristen L; Burris, Kellie P; Zivanovic, Svetlana; Davidson, P Michael; Stewart, C Neal

    2014-02-01

    Hibiscus sabdariffa L. calyces are widely used in the preparation of beverages. The calyces contain compounds that exhibit antimicrobial activity, yet little research has been conducted on their possible use in food systems as antimicrobials. Aqueous extracts prepared from the brand "Mi Costenita" were sterilized by membrane filtration (0.22-μm pore size) or autoclaving (121 °C, 30 min) and tested for antimicrobial activity against the foodborne pathogens Escherichia coli O157:H7 strains ATCC 43894 and Cider and Staphylococcus aureus strains SA113 and ATCC 27708 in a microbiological medium and ultrahigh-temperature-processed milk with various fat percentages. Extracts heated by autoclaving exhibited greater activity than did filtered extracts in a microbiological medium. Against E. coli, results of 20 mg/ml filtered extract were not different from those of the control, whereas autoclaved extracts reduced viable cells ca. 3 to 4 log CFU/ml. At 60 mg/ml, both extracts inactivated cells after 24 h. There were reduced populations of both strains of S. aureus (ca. 2.7 and 3 log CFU/ml, respectively) after 24 h of incubation in 40 mg/ml filtered extracts. When grown in autoclaved extracts at 40 mg/ml, both strains of S. aureus were inactivated after 9 h. Autoclaved extracts had decreased anthocyanin content (2.63 mg/liter) compared with filtered extracts (14.27 mg/liter), whereas the phenolic content (48.7 and 53.8 mg/g) remained similar for both treatments. Autoclaved extracts were then tested for activity in milk at various fat concentrations (skim [3.25%]) against a 1:1 mixture of the two strains of E. coli O157:H7 and a 1:1 mixture of the two strains of S. aureus. Extracts at 40 mg/ml inactivated S. aureus after 168 h in skim and whole milk, and E. coli was inactivated after 96 h in 60 mg/ml extract in all fat levels. These findings show the potential use of Hibiscus extracts to prevent the growth of pathogens in foods and beverages.

  17. Herbal products containing Hibiscus sabdariffa L., Crataegus spp., and Panax spp.: Labeling and safety concerns.

    Science.gov (United States)

    Nunes, Maria Antónia; Rodrigues, Francisca; Alves, Rita C; Oliveira, Maria Beatriz P P

    2017-10-01

    Herbs have been used from ancient times for infusion preparation based on their potential health effects. In particular, the consumption of Hibiscus sabdariffa L., Crataegus spp. and Panax spp. has been largely associated to cardiovascular benefits. In this work, the label information of 52 herbal products for infusion preparation containing the referred herbs was analyzed and discussed, taking into consideration the European Union regulation for herbal products, which intends to protect public health and harmonize the legal framework in Member States. Details about the cardiovascular-related statements and warning notifications about consumption were considered. Also, regulatory issues and possible herb-drug interactions were explored and discussed. A total of 14 of the 52 herbal products selected presented health claims/statements on the label. Hibiscus was present in the majority of the products and, in some cases, it was mentioned only in the ingredients list and not on the product front-of-pack. Despite the promising outcomes of these plants to modulate cardiovascular risk markers, consumers with some sort of cardiovascular dysfunction and/or under medication treatments should be aware to carefully analyze the labels and consult additional information related to these herbal products. Manufacturers have also a huge responsibility to inform consumers by presenting awareness statements. Lastly, health professionals must advise and alert their patients about possible interactions that could occur between the concomitant consumption of drugs and herbs. Overall, there is still a real need of additional studies and clinical trials to better understand herbs effects and establish a science-based guidance to assess their safety. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Occurrence of Root Rot and Vascular Wilt Diseases in Roselle (Hibiscus sabdariffa L.) in Upper Egypt.

    Science.gov (United States)

    Hassan, Naglaa; Shimizu, Masafumi; Hyakumachi, Mitsuro

    2014-03-01

    Roselle (Hibiscus sabdariffa L.) family Malvaceae is an important crop used in food, cosmetics and pharmaceutics industries. Roselle is cultivated mainly in Upper Egypt (Qena and Aswan governorates) producing 94% of total production. Root rot disease of roselle is one of the most important diseases that attack both seedlings and adult plants causing serious losses in crop productivity and quality. The main objective of the present study is to identify and characterize pathogens associated with root rot and wilt symptoms of roselle in Qena, Upper Egypt and evaluate their pathogenicity under greenhouse and field condition. Fusarium oxysporum, Macrophomina phaseolina, Fusarium solani, Fusarium equiseti and Fusarium semitectum were isolated from the natural root rot diseases in roselle. All isolated fungi were morphologically characterized and varied in their pathogenic potentialities. They could attack roselle plants causing damping-off and root rot/wilt diseases in different pathogenicity tests. The highest pathogenicity was caused by F. oxysporum and M. phaseolina followed by F. solani. The least pathogenic fungi were F. equiseti followed by F. semitectum. It obviously noted that Baladi roselle cultivar was more susceptible to infection with all tested fungi than Sobhia 17 under greenhouse and field conditions. This is the first report of fungal pathogens causing root rot and vascular wilt in roselle in Upper Egypt.

  19. Antitumoral Effect of Hibiscus sabdariffa on Human Squamous Cell Carcinoma and Multiple Myeloma Cells.

    Science.gov (United States)

    Malacrida, Alessio; Maggioni, Daniele; Cassetti, Arianna; Nicolini, Gabriella; Cavaletti, Guido; Miloso, Mariarosaria

    2016-10-01

    Cancer is a leading cause of death worldwide. Despite therapeutic improvements, some cancers are still untreatable. Recently there has been an increasing interest in the use of natural substances for cancer prevention and treatment. Hibiscus sabdariffa (HS) is a plant, belonging to Malvaceae family, widespread in South Asia and Central Africa. HS extract (HSE) used in folk medicine, gained researchers' interest thanks to its antioxidant, anti-inflammatory, and chemopreventive properties. In the present study, we initially assessed HSE effect on a panel of human tumor cell lines. Then we focused our study on the following that are most sensitive to HSE action cell lines: Multiple Myeloma (MM) cells (RPMI 8226) and Oral Squamous Cell Carcinoma (OSCC) cells (SCC-25). In both RPMI 8226 and SCC-25 cells, HSE impaired cell growth, exerted a reversible cytostatic effect, and reduced cell motility and invasiveness. We evaluated the involvement of MAPKs ERK1/2 and p38 in HSE effects by using specific inhibitors, U0126 and SB203580, respectively. For both SCC-25 and RPMI 8226, HSE cytostatic effect depends on p38 activation, whereas ERK1/2 modulation is crucial for cell motility and invasiveness. Our results suggest that HSE may be a potential therapeutic agent against MM and OSCC.

  20. Hibiscus sabdariffa extract inhibits obesity and fat accumulation, and improves liver steatosis in humans.

    Science.gov (United States)

    Chang, Hong-Chou; Peng, Chiung-Huei; Yeh, Da-Ming; Kao, Erl-Shyh; Wang, Chau-Jong

    2014-04-01

    Obesity is associated with a great diversity of diseases including non-alcoholic fatty liver disease. Our previous report suggested that Hibiscus sabdariffa extracts (HSE) had a metabolic-regulating and liver-protecting potential. In this study, we performed a clinical trial to further confirm the effect of HSE. Subjects with a BMI ≧ 27 and aged 18-65, were randomly divided into control (n = 17) and HSE-treated (n = 19) groups, respectively, for 12 weeks. Our data showed that consumption of HSE reduced body weight, BMI, body fat and the waist-to-hip ratio. Serum free fatty acid (FFA) was lowered by HSE. Anatomic changes revealed that HSE improved the illness of liver steatosis. Ingestion of HSE was well tolerated and there was no adverse effect during the trial. No alteration was found for serum α-amylase and lipase. The clinical effect should mainly be attributed to the polyphenols of HSE, since composition analysis showed that branched chain-amino acids, which is associated with obesity, is not obviously high. In conclusion, consumption of HSE reduced obesity, abdominal fat, serum FFA and improved liver steatosis. HSE could act as an adjuvant for preventing obesity and non-alcoholic fatty liver.

  1. Entomofauna asociada al cultivo de jamaica (Hibiscus sabdariffa L.) en el municipio de Chiautla de Tapia, Puebla

    OpenAIRE

    Betzabeth Cecilia PÉREZ TORRES; Agustín ARAGÓN GARCÍA; Néstor BAUTISTA MARTÍNEZ; Tapia Rojas, Ana María.; Jesús Francisco LÓPEZ-OLGUÍN

    2009-01-01

    En Chiautla de Tapia, Puebla, mediante muestreos realizados cada 15 días de junio a septiembre del 2007, se efectuaron colectas en tres parcelas cultivadas con jamaica (Hibiscus sabdariffa L.), utilizando un aspirador entomológico manual y una red de golpeo. Se revisaron los tallos, nudos, entre nudos, hojas (haz y envés) y flores para hacer un diagnóstico de la entomofauna. Un total de 1,856 ejemplares fueron colectados, de los cuales se determinaron 17 especies, comprendidas en 6 órdenes, 1...

  2. Antioxidant Activity of Leaf Extracts from Different Hibiscus sabdariffa Accessions and Simultaneous Determination Five Major Antioxidant Compounds by LC-Q-TOF-MS

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    Jin Wang

    2014-12-01

    Full Text Available Hibiscus sabdariffa has gained attention for its antioxidant activity. There are many accessions of H. sabdariffa in the world. However, information on the quantification of antioxidant compounds in different accessions is rather limited. In this paper, a liquid chromatography/quadrupole-time-of-flight mass spectrometry (LC-Q-TOF-MS method for simultaneous determination of five antioxidant compounds (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, and isoquercitrin in H. sabdariffa leaves was developed. The method was validated for linearity, sensitivity, precision, repeatability and accuracy. The validated method has been successfully applied for determination of the five analytes in eight accessions of H. sabdariffa. The eight accessions of H. sabdariffa were evaluated for their antioxidant activities by DPPH free radical scavenging assay. The investigated accessions of H. sabdariffa were rich in rutin and exhibited strong antioxidant activity. The two accessions showing the highest antioxidant activities were from Cuba (No. 2 and Taiwan (No. 5. The results indicated that H. sabdariffa leaves could be considered as a potential antioxidant source for the food industry. The developed LC-Q-TOF-MS method is helpful for quality control of H. sabdariffa.

  3. Thermoluminescence response of the poly mineral fraction from hibiscus sabdariffa L foodstuffs

    Energy Technology Data Exchange (ETDEWEB)

    Cruz Z, E.; Guzman, S. [UNAM, Instituto de Ciencias Nucleares, Apdo. Postal 70-543, 04510 Mexico D. F. (Mexico); Brown, F. [Universidad de Sonora, Departamento de Investigacion en Polimeros y Materiales, Apdo. Postal 130, 83000 Hermosillo, Sonora (Mexico); Chernov, V.; Barboza F, M., E-mail: ecruz@nucleares.unam.m [Universidad de Sonora, Departamento de Investigacion en Fisica, Apdo. Postal 5-088, 83190 Hermosillo, Sonora (Mexico)

    2011-02-15

    Food processed by ionizing irradiation is a safe technology and has been recognized by the FAO/Who Codes Alimentarius Commission. It is an excellent method to prevent food spoilage and food borne diseases by inhibiting the growth of microorganisms and slowing down ripening. The widespread use of food irradiation treatments that include spices, dry vegetables, grains and fruits make relevant the developing of methods for identification and analyses of foodstuffs processed by irradiation. The present work focuses on the thermoluminescence property of Mexican Roselle flower previously irradiated for detection purposes. The poly mineral content of irradiated commercial Roselle flower (Hibiscus sabdariffa L.) was extracted and analyzed by thermoluminescence (Tl). The X-ray diffraction analyses showed that quartz and albite composition for the poly mineral fraction. Different grain sizes; 10, 53, 74 and 149 {mu}m, were selected for the Tl analyses. The Tl glow curves depended on the grain sizes. The glow curves depicted two peaks around 92 and 120 C. The first peak was ascribed to quartz and the broad part of the glow curves (120-250 C) seems to correspond to the albite. Because the complex structure of the Tl glow curves from poly minerals the kinetic parameters were calculated by a fitting process using a deconvolution method based on a non-linear least-squares Levenberg-Ma quart. The values of the activation energy were found to be at 0.79-1.05 eV and 0.79-1.04 for 53 {mu}m and 250 {mu}m, respectively. The Tl properties of the samples were determined including dose response, reproducibly, fading and UV light bleaching. (Author)

  4. Functional and antioxidant properties of novel snack crackers incorporated with Hibiscus sabdariffa by-product

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    Zahra S. Ahmed

    2015-01-01

    Full Text Available The Hibiscus sabdariffa calyxes’ residue (HSR remained after the extraction of beverage is discarded which contributes to environmental pollution. The objective of this study was to explore the suitability of incorporating different amount of HSR (0%, 1.25%, 2.5%, 3.75%, and 5.0% in crackers to enhance dietary fiber and antioxidant content. Physicochemical properties, antioxidants activity, nutritional quality, sensory profile and microstructure properties of samples containing HSR were examined and compared with control crackers. Cracker protein and fat levels decreased as HSR increased from 0.0% to 5% while ash increased. The total dietary fiber DF increased from 3.36% to 8.17% where the highest DF was reached at 5% HSR. The content of phenols increased from 5.99 to 17.57 mg/g and total flavonoid content increased from 49.36 to 104.63 mg/g of crackers incorporated with 5% HSR. DPPH radical scavenging activity increased two fold by increasing HSR up to 5%. HSR containing crackers exhibited darker L values than none/less HSR containing ones. In sensory ranking tests, acceptable crackers with pleasant flavor were obtained by incorporating up to 3.75% HSR into the cracker’s formula. Crackers prepared with 5% HSR received the poorest sensory rating compared to non/less HSR enriched cracker. Scanning electron microscopy (EM images of the prepared crackers revealed marked changes caused by incorporating HSR as upon HSR addition the surface was observed to be scratched, cracker and rougher. Overall results suggest that HSR is a potential functional food ingredient high in fiber content and antioxidants activity that may be processed into flour and used in food applications, such as baked goods.

  5. Antioxidant and cytotoxic effect of Barringtonia racemosa and Hibiscus sabdariffa fruit extracts in MCF-7 human breast cancer cell line

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    Norliyana Amran

    2016-01-01

    Full Text Available Background: The fruits of Barringtonia racemosa and Hibiscus sabdariffa have been used in the treatment of abscess, ulcer, cough, asthma, and diarrhea as traditional remedy. Objective: This study aims to evaluate cytotoxic effect of B. racemosa and H. sabdariffa methanol fruit extracts toward human breast cancer cell lines (MCF-7 and its antioxidant activities. Materials and Methods: Total antioxidant activities of extracts were assayed using 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH and β-carotene bleaching assay. Content of phytochemicals, total flavonoid content (TFC, and total phenolic content (TPC were determined using aluminum chloride colorimetric method and Folin-Ciocalteu′s reagent, respectively. Cytotoxic activity in vitro was investigated through 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide (MTT assay. Results: B. racemosa extract exhibited high antioxidant activities compared to H. sabdariffa methanol fruit extracts in DPPH radical scavenging assay (inhibitory concentration [IC 50 ] 15.26 ± 1.25 μg/mL and ί-carotene bleaching assay (I% 98.13 ± 1.83%. B. racemosa also showed higher TPC (14.70 ± 1.05 mg gallic acid equivalents [GAE]/g and TFC (130 ± 1.18 mg quercetin equivalents [QE]/g compared to H. sabdariffa (3.80 ± 2.13 mg GAE/g and 40.75 ± 1.15 mg QE/g, respectively. In MTT assay, B. racemosa extract also showed a higher cytotoxic activity (IC 50 57.61 ± 2.24 μg/mL compared to H. sabdariffa. Conclusion: The present study indicated that phenolic and flavonoid compounds known for oxidizing activities indicated an important role among the contents of these plants extract. B. racemosa methanol extract have shown potent cytotoxic activity toward MCF-7. Following these promising results, further fractionation of the plant extract is underway to identify important phytochemical bioactives for the development of potential nutraceutical and pharmaceutical use.

  6. STUDIES ON HIBISCUS CANNABINUS, HIBISCUS SABDARIFFA, AND CANNABINUS SATIVA PULP TO BE A SUBSTITUTE FOR SOFTWOOD PULP- PART 2: SAS-AQ AND NSSC-AQ DELIGNIFICATION PROCESSES

    Directory of Open Access Journals (Sweden)

    Dharm Dutt

    2010-08-01

    Full Text Available Hibiscus cannabinus, Cannabis sativa, and Hibiscus sabdariffa, fast growing productive annual plants, could provide fiber necessary to partially alleviate the world’s fiber deficit. The present study aimed at producing high yield pulp and the best mechanical strength properties with minimum impact on environment by SAS-AQ, and NSSC-AQ pulping processes. A total alkali of 13% (as Na2O, an alkali ratio of 0.80, and a Na2SO3 charge 11.70% (as Na2O were found optimum to reduce maximum kappa number. A lower kappa number and good strength properties were achieved by increasing total alkali and Na2SO3 charge. SAS-AQ pulps showed good response towards CEHH bleaching. The NSSC-AQ pulping was conducted at a total alkali charge of 8% (as Na2O by varying the ratio of sulphite-to-carbonate (100:0-0:100, and cooking time (60-120 min at 1600C. A ratio of sulphite-to-carbonate 60:40 was suitable for corrugating medium (cooking time 60 min, while a ratio of sulphite-to-carbonate 70:30 showed better strength properties (longer cooking time.

  7. Effect of Hibiscus sabdariffa L. Dried Calyx Ethanol Extract on Fat Absorption-Excretion, and Body Weight Implication in Rats

    Science.gov (United States)

    Carvajal-Zarrabal, O.; Hayward-Jones, P. M.; Orta-Flores, Z.; Nolasco-Hipólito, C.; Barradas-Dermitz, D. M.; Aguilar-Uscanga, M. G.; Pedroza-Hernández, M. F.

    2009-01-01

    The effect of Hibiscus sabdariffa L. (Hs) calyx extract on fat absorption-excretion and body weight in rats, was investigated. Rats were fed with either a basal diet (SDC = Control diet) or the same diet supplemented with Hs extracts at 5%, 10% and 15% (SD5, SD10 and SD15). Only SD5 did not show significant increases in weight, food consumption and efficiency compared to SDC. The opposite occurred in SD15 group which showed a significant decrease for these three parameters. The SD10 responses were similar to SD15, with the exception of food consumption. In both SDC and SD5 groups, no body weight loss was observed; however, only in the latter group was there a significantly greater amount of fatty acids found in feces. A collateral effect emerging from the study is that components of Hs extract at the intermediate and greater concentrations used in this experiment could be considered possible antiobesity agents. PMID:19756159

  8. Pengaruh Pemberian Ekstrak Rosella (Hibiscus sabdariffa Linn Terhadap Kadar Malondialdehid dan Aktivitas Katalase Tikus yang Terpapar Karbon Tetraklorida

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    Zuraida ,

    2015-09-01

    Full Text Available Abstrak Pemberian rosella (Hibiscus sabdariffa Linn diharapkan melindung hepar tikus dari kerusakkan akibat stres oksidatif pada keracunan karbon tetraklorida (CCl 4. Senyawa yang sering dijadikan petunjuk adanya kerusakan tersebut adalah malondialdehid (MDA. Rosella mengandung vitamin C, flavonoid, polifenol dan beta karoten. Tujuanpenelitian ini adalah menentukan pengaruh pemberian ekstrak rosella (Hibiscus sabdariffa Linn terhadap MDA dan aktivitas katalase tikus yang terpapar CCl 4. Ini adalah penelitian eksperimental dengan desain Post test Only Control Group Design. Sampel 24 ekor tikus Strain Wistar berumur 2-3 bulan, berat 150-200 gr. Sampel diambil secara acakdan dibagi 4 kelompok terdiri dari kelompok kontrol negatif, kontrol positif (CCl 4, perlakuan 1 (CCl 4 dan ekstrak rosella 250 mg/kg bb dan perlakuan 2 (CCl4  dan ekstrak rosella 500 mg/kg bb. Pemberian CCl 4secara oral dosis tunggal, setelah 24 jam kemudian diberi ekstrak rosella secara oral selama 14 hari. Data dianalisis dengan uji Anova, tingkatkepercayaan 95%.Pemberian ekstrak rosella secara statistik didapatkan perbedaan yang signifikan rerata kadar MDA dan katalase antar kelompok (p < 0,05. Disimpulkan bahwa ekstrak rosella dapat menurunkan kadar MDA dan meningkatkan aktivitas katalase tikus yang terpapar CCl 4. Kata kunci: karbon tetraklorida, MDA, katalase, rosella Abstract Administering roselle (Hibiscus sabdariffa Linn is expected to protect rat liver from damage caused by oxidative stress in CCl4 poisoning. Rosella contains vitamin C, flavonoids, polyphenol and beta carotene. Compounds which was often used as marker of the damage caused by free radicals wa MDA. The objective of this study was to determine the effect of extracts of roselle (Hibiscus sabdariffa Linn on MDA and catalase activity of rats exposed to CCl4. Experimental research design with Post test Only Control Group Design. Samples of 24 male Wistar Strain rats were 2-3 months old. weighing 150-200 gr

  9. Pengaruh Konsentrasi Gula dan Ragi Dalam Pembuatan Cuka Dari Rosella (Hibiscus sabdariffa.L Terhadap Mutu Cuka Rosella

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    Firdausni Firdausni

    2013-12-01

    Full Text Available Rosellla (Hibiscus sabdariffa.L has been traditionally used as nutritious functional beverages. Rosella flowers contain flavonoid secondary metabolites, terpenoids, and vitamin C, which are considered as antioxidant. Research aims to see the effect of sugar and yeast  on quality of vinegar. The study was conducted with the treatment of sugar utilization (10, 15, 20, 25 %, fermentation with yeast Saccharomyces cerevisiae (2, 4, 6 g and fermentation time until three weeks. Rosella vinegar as a results of fermentation further was analyzed the pH, acetic acid content, extract content and antioxidant activity test consisted of qualitative test of phenolic, flavonoid, and % inhibition. Result of the optimal research was obtained in the treatment of sugar utilization 20% and the addition of yeast 6 g with analysis result of pH 2.67, acetic acid 14.80%, extract content 7.15%, and % inhibition 31%, moreover it contained phenolic and flavonoidscompounds qalitatively.ABSTRAKRosellla (Hibiscus sabdariffa.L telah digunakan secara tradisional sebagai minuman yang berkhasiat fungsional. Bunga rosella mengandung flavonoid metabolit sekunder, terpenoid, dan vitamin C yang berfungsi sebagai antioksidan. Penelitian ini bertujuan untuk melihat pengaruh pemakaian gula dan ragi  terhadap mutu cuka. Penelitian dilakukan dengan perlakuan penggunaan gula pasir (10, 15, 20, 25 %, fermentasi dengan ragi Saccharomyces cerevisiae (2, 4, 6 g dan waktu fermentasi  sampai tiga minggu. Hasil fermentasi berupa cuka rosella selanjutnya diuji pH, kandungan asam asetat, kadar sari dan uji aktifitas antioksidan yang terdiri dari uji kualitatif fenolik, flavonoid, dan % inhibisi. Hasil penelitian optimal diperoleh pada perlakuan penggunaan gula 20% dan penambahan ragi 6 g dengan hasil analisis pH 2,67, asam asetat 14,80%, kadar sari 7,15% dan % inhibisi 31% serta secara kualitatif mengandung senyawaan fenolik dan flavonoid.

  10. Awareness and utilization of “Sobo” ( Hibiscus sabdariffa-roselle ) by ...

    African Journals Online (AJOL)

    “Sobo” is an indigenous non-alcoholic drink made from hot extract of Roselle calyces ... It was recommended that extension activities should be invigorated, with a ... and at the expense of carbonated soft drinks with it usual high sugar content.

  11. Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS.

    Science.gov (United States)

    Borrás-Linares, Isabel; Herranz-López, María; Barrajón-Catalán, Enrique; Arráez-Román, David; González-Álvarez, Isabel; Bermejo, Marival; Fernández Gutiérrez, Alberto; Micol, Vicente; Segura-Carretero, Antonio

    2015-08-07

    Previous findings on the capacity of Hibiscus sabdariffa (HS) polyphenols to ameliorate metabolic disturbances justify the necessity of studies oriented to find the potential metabolites responsible for such an effect. The present study examined the intestinal epithelial membrane permeability of polyphenols present in a phenolic-enriched Hibiscus sabdariffa extract (PEHS), free and encapsulated, using the Caco-2 cell line. Additionally, selected polyphenols (quercetin, quercetin-3-glucoside, quercetin-3-glucuronide, and N-feruloyltyramine) were also studied in the same absorption model. The powerful analytical platform used ultra-high-performance liquid chromatography coupled with ultra-high-resolution quadrupole time-of-flight mass spectrometry (UHPLC-ESI-UHR-Qq-TOF-MS), and enabled the characterization of seven new compounds in PEHS. In the permeation study, only a few compounds were able to cross the cell monolayer and the permeability was lower when the extract was in an encapsulated form. Pure compounds showed a moderate absorption in all cases. Nevertheless, these preliminary results may need further research to understand the complete absorption mechanism of Hibiscus polyphenols.

  12. Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS

    Directory of Open Access Journals (Sweden)

    Isabel Borrás-Linares

    2015-08-01

    Full Text Available Previous findings on the capacity of Hibiscus sabdariffa (HS polyphenols to ameliorate metabolic disturbances justify the necessity of studies oriented to find the potential metabolites responsible for such an effect. The present study examined the intestinal epithelial membrane permeability of polyphenols present in a phenolic-enriched Hibiscus sabdariffa extract (PEHS, free and encapsulated, using the Caco-2 cell line. Additionally, selected polyphenols (quercetin, quercetin-3-glucoside, quercetin-3-glucuronide, and N-feruloyltyramine were also studied in the same absorption model. The powerful analytical platform used ultra-high-performance liquid chromatography coupled with ultra-high-resolution quadrupole time-of-flight mass spectrometry (UHPLC-ESI-UHR-Qq-TOF-MS, and enabled the characterization of seven new compounds in PEHS. In the permeation study, only a few compounds were able to cross the cell monolayer and the permeability was lower when the extract was in an encapsulated form. Pure compounds showed a moderate absorption in all cases. Nevertheless, these preliminary results may need further research to understand the complete absorption mechanism of Hibiscus polyphenols.

  13. Multi-Targeted Molecular Effects of Hibiscus sabdariffa Polyphenols: An Opportunity for a Global Approach to Obesity

    Science.gov (United States)

    Herranz-López, María; Olivares-Vicente, Mariló; Barrajón-Catalán, Enrique; Segura-Carretero, Antonio; Joven, Jorge; Micol, Vicente

    2017-01-01

    Improper diet can alter gene expression by breaking the energy balance equation and changing metabolic and oxidative stress biomarkers, which can result in the development of obesity-related metabolic disorders. The pleiotropic effects of dietary plant polyphenols are capable of counteracting by modulating different key molecular targets at the cell, as well as through epigenetic modifications. Hibiscus sabdariffa (HS)-derived polyphenols are known to ameliorate various obesity-related conditions. Recent evidence leads to propose the complex nature of the underlying mechanism of action. This multi-targeted mechanism includes the regulation of energy metabolism, oxidative stress and inflammatory pathways, transcription factors, hormones and peptides, digestive enzymes, as well as epigenetic modifications. This article reviews the accumulated evidence on the multiple anti-obesity effects of HS polyphenols in cell and animal models, as well as in humans, and its putative molecular targets. In silico studies reveal the capacity of several HS polyphenols to act as putative ligands for different digestive and metabolic enzymes, which may also deserve further attention. Therefore, a global approach including integrated and networked omics techniques, virtual screening and epigenetic analysis is necessary to fully understand the molecular mechanisms of HS polyphenols and metabolites involved, as well as their possible implications in the design of safe and effective polyphenolic formulations for obesity. PMID:28825642

  14. Development of functional beverages from blends of Hibiscus sabdariffa extract and selected fruit juices for optimal antioxidant properties.

    Science.gov (United States)

    Ogundele, Oluwatoyin M A; Awolu, Olugbenga O; Badejo, Adebanjo A; Nwachukwu, Ifeanyi D; Fagbemi, Tayo N

    2016-09-01

    The demand for functional foods and drinks with health benefit is on the increase. The synergistic effect from mixing two or more of such drinks cannot be overemphasized. This study was carried out to formulate and investigate the effects of blends of two or more of pineapple, orange juices, carrot, and Hibiscus sabdariffa extracts (HSE) on the antioxidant properties of the juice formulations in order to obtain a combination with optimal antioxidant properties. Experimental design was carried out using optimal mixture model of response surface methodology which generated twenty experimental runs with antioxidant properties as the responses. The DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] radical scavenging abilities, ferric reducing antioxidant potential (FRAP), vitamin C, total phenolics, and total carotenoids contents of the formulations were evaluated as a test of antioxidant property. In all the mixtures, formulations having HSE as part of the mixture showed the highest antioxidant potential. The statistical analyzes, however, showed that the formulations containing pineapple, carrot, orange, and HSE of 40.00, 16.49, 17.20, and 26.30%, respectively, produced optimum antioxidant potential and was shown to be acceptable to a research laboratory guidance panel, thus making them viable ingredients for the production of functional beverages possessing important antioxidant properties with potential health benefits.

  15. Planting time for maximization of yield of vinegar plant calyx (Hibiscus sabdariffa L. Época de plantio para maximização daprodução de cálices de vinagreira (Hibiscus sabdariffa L.

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    Nilmar Eduardo Arbex de Castro

    2004-06-01

    Full Text Available The objective of this work was to evaluate the yield of calyxes of Hibiscus sabdariffa L, a medicinal plant, at four planting times in Lavras - MG. The treatments were four planting times (October 18th, November 15th, December 18th 2001 and January 15th 2002 and a harvest was proceeded when practically there were no developing calyxes, almost at the end of the plant cycle. The numbers of calyxes per plant, the fresh and dry biomasses of calyxes and quality were taken into account. It follows that planting time influenced yield per plant and the fresh and dry biomasses of calyxes, differing from each other by Tukey test at 5%. In October planting, there was a higher yield (2,522kg/ha with a yield 5.24 as high relative to the planting of the month of January (481 kg/ha. The planting s in the month of November to December showed yields of 1,695 and 1,093 kg/ha of dry calyxes, respectively and in relation to the same months of January, yield was of 3.52 to 2.27 times more. Harvest must be done as soon as the calyxes are ripe in order to preserve quality.Objetivou-se avaliar a produtividade de cálices de Hibiscus sabdariffa L., planta medicinal, em quatro épocas de plantio em Lavras - M.G. Os tratamentos foram quatro épocas de plantio (18 de outubro; 15 de novembro; 18 de dezembro de 2001 e 15 de janeiro de 2002 e realizada uma colheita quando praticamente não existiam cálices em desenvolvimento, quase no final do ciclo da planta. Foram considerados os números de cálices por planta, as fitomassas frescas e secas dos cálices e a qualidade. Concluiu-se que a época de plantio influenciou o rendimento por planta e as fitomassas frescas e secas dos cálices, diferindo entre si pelo teste de Tukey a 5%. No plantio de outubro, houve maior rendimento (2.522 kg/ha, com produção de 5,24 vezes a mais em relação ao plantio do mês de janeiro (481 kg/ha. Os plantios nos meses de novembro e dezembro tiveram produções de 1.695 e 1.093 kg.ha-1 de c

  16. Effect of Hibiscus sabdariffa extract on high fat diet–induced obesity and liver damage in hamsters

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    To-Wei Huang

    2015-10-01

    Full Text Available Background: Obesity is a chronic metabolic disorder associated with an increase in adipogenesis and often accompanied with fatty liver disease. Objective: In this study, we investigated the anti-obesity effects of Hibiscus sabdariffa water extract (HSE in vivo. Method: Eight-weeks-old male mice were divided into six groups (n=8 per group and were fed either normal feed, a high fat diet (HFD, HFD supplemented with different concentrations of HSE, or HFD supplemented with anthocyanin. After 10 weeks of feeding, all the blood and livers were collected for further analysis. Results: Mesocricetus auratus hamster fed with a high-fat diet developed symptoms of obesity, as determined from their body weight change and from their plasma lipid levels. Meanwhile, HSE treatment reduced fat accumulation in the livers of hamsters fed with HFD in a concentration-dependent manner. Administration of HSE reduced the levels of liver cholesterol and triglycerides, which were elevated by HFD. Analysis of the effect of HSE on paraoxonase 1, an antioxidant liver enzyme, revealed that HSE potentially regulates lipid peroxides and protects organs from oxidation-associated damage. The markers of liver damage such as serum alanine aminotransferase and aspartate aminotransferase levels that were elevated by HFD were also reduced on HSE treatment. The effects of HSE were as effective as treatment with anthocyanin; therefore the anthocyanins present in the HSE may play a crucial role in the protection established against HFD-induced obesity. Conclusions: In conclusion HSE administration constitutes an effective and viable treatment strategy against the development and consequences of obesity.

  17. Processing Effects on the Antioxidant Activities of Beverage Blends Developed from Cyperus esculentus, Hibiscus sabdariffa, and Moringa oleifera Extracts.

    Science.gov (United States)

    Badejo, Adebanjo A; Damilare, Akintoroye; Ojuade, Temitope D

    2014-09-01

    The discovery of bioactive compounds in foods has changed the dietary lifestyle of many people. Cyperus esculentus (tigernut) is highly underutilized in Africa, yet tigernut extract is highly profitable in Europe. This study aims to add value to tigernut extract by revealing its health benefits and food value. In this study, tigernut tubers were germinated or roasted and the extracts were combined with Moringa oleifera extract (MOE) or Hibiscus sabdariffa extract (HSE) and spiced with ginger to produce functional drinks. The drinks were evaluated for physicochemical characteristics, sensory parameters, and antioxidant potentials. The total phenolic content of each beverage was measured by the Folin-Ciocalteu method, and the antioxidant activity of each beverage was determined by the 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid assays. The beverages from the germinated tigernut extracts had the highest titratable acidity and the lowest pH, while beverages containing the roasted tigernut extract had the highest ∘Brix. Germination and roasting significantly enhanced the total phenolic content of the drinks. The beverage containing HSE and germinated tigernut extract had a total phenolic content of 45.67 mg/100 mL gallic acid equivalents, which was significantly higher than the total phenolic content of all other samples. The DPPH inhibition activity of the beverages prepared with germinated tigernut extracts was significantly higher than the DPPH inhibition activity of the beverages prepared with fresh tigernut extract. The taste and overall acceptability of drinks containing the roasted tigernut extract were preferred, while the color and appearance of drinks with the germinated samples were preferred. Roasting or germinating tigernuts before extraction and addition of MOE or HSE extracts is another way to add value and enhance the utilization of tigernuts.

  18. Hibiscus sabdariffa increases hydroxocobalamin oral bioavailability and clinical efficacy in vitamin B12 deficiency with neurological symptoms.

    Science.gov (United States)

    Souirti, Zouhayr; Loukili, Mouna; Soudy, Imar D; Rtibi, Kaies; Özel, Aslihan; Limas-Nzouzi, Nicolas; El Ouezzani, Seloua; Eto, Bruno

    2016-12-01

    The aim of the study was to evaluate the bioavailability and clinical benefits of oral new formulation (HB12 ) of hydroxocobalamin (Hdrx) with Hibiscus sabdariffa (HS). First, in an observational study, a cohort of 30 vitamin B12 -deficient patients (vit B12 vitamin B12 level increased from 55.1 ± 36.9 to 1330 ± 335.5 pg/mL at day 10 and 431.0 ± 24.27 pg/mL at day 90, without overt adverse effects. In mice ileum, (i) intestinal bioavailability of Hdrx increased in dose-dependent manner with HB12 . The apparent permeability of Hdrx was Papp = 34.9 ± 4.6 × 10(-6) cm/s in the presence of 3 mg/mL (HB12 B) compared to the control Papp = 6.2 ± 0.7 × 10(-6) cm/s. (ii) Total transepithelial electrical conductance (Gt ) increased in dose-dependent manner with HB12 , Gt = 161.5 ± 10.8 mS/cm² with HB12 B (Hdrx 1 mg + HS 3 mg) compared to the control Hdrx, Gt = 28.7 ± 4.0 mS/cm². In conclusion, the clinical study suggests that injections are not required when Hdrx is given orally. Intestinal bioavailability of Hdrx increased in vitro when it was used concomitantly with HS. © 2016 Société Française de Pharmacologie et de Thérapeutique.

  19. Análisis comparativo de compuestos fenólicos totales y actividad antioxidante de cuatro marcas de tisanas de Hibiscus sabdariffa (Malvaceae comercializadas en Costa Rica

    Directory of Open Access Journals (Sweden)

    Melissa Agüero

    2014-01-01

    Full Text Available Las tisanas de rosa de Jamaica (Hibiscus sabdariffa son consumidas en Costa Rica por su conocido efecto cardioprotector asociado a la presencia de compuestos fenólicos, principalmente antocianinas. Este trabajo estableció diferencias en el perfil de compuestos fenólicos y su actividad anti-radical entre diferentes comercializadores de tisanas de Hibiscus sabdariffa en Costa Rica. Se identificaron cualitativamente taninos, flavonoides y antocianinas. Se cuantificó la cantidad total de compuestos fenólicos por el método de  Folin-Ciocalteu y se cuantificó la actividad antioxidante con el método de DPPH. Todas las marcas comerciales demostraron presencia de compuestos fenólicos, incluyendo antocianinas; sin embargo, se encontraron diferencias significativas en el contenido de compuestos fenólicos y actividad anti-radical, entre los diferentes productores de tisanas. Por ello, se concluye que en el mercado costarricense, el perfil en compuestos fenólicos de las tisanas en estudio varía de acuerdo con el productor.

  20. Aqueous Extract from Hibiscus sabdariffa Linnaeus Ameliorate Diabetic Nephropathy via Regulating Oxidative Status and Akt/Bad/14-3-3γ in an Experimental Animal Model

    Directory of Open Access Journals (Sweden)

    Shou-Chieh Wang

    2011-01-01

    Full Text Available Several studies point out that oxidative stress maybe a major culprit in diabetic nephropathy. Aqueous extract of Hibiscus sabdariffa L. (HSE has been demonstrated as having beneficial effects on anti-oxidation and lipid-lowering in experimental studies. This study aimed at investigating the effects of Hibiscus sabdariffa L. on diabetic nephropathy in streptozotocin induced type 1 diabetic rats. Our results show that HSE is capable of reducing lipid peroxidation, increasing catalase and glutathione activities significantly in diabetic kidney, and decreasing the plasma levels of triglyceride, low-density lipoprotein (LDL and increasing high-density lipoprotein (HDL value. In histological examination, HSE improves hyperglycemia-caused osmotic diuresis in renal proximal convoluted tubules (defined as hydropic change in diabetic rats. The study also reveals that up-regulation of Akt/Bad/14-3-3γ and NF-κB-mediated transcription might be involved. In conclusion, our results show that HSE possesses the potential effects to ameliorate diabetic nephropathy via improving oxidative status and regulating Akt/Bad/14-3-3γ signaling.

  1. An evaluation of the hypolipidemic effect of an extract of Hibiscus Sabdariffa leaves in hyperlipidemic Indians: a double blind, placebo controlled trial

    Directory of Open Access Journals (Sweden)

    R Rajendran

    2010-06-01

    Full Text Available Abstract Background Hibiscus sabdariffa is used regularly in folk medicine to treat various conditions. Methods The study was a double blind, placebo controlled, randomized trial. Sixty subjects with serum LDL values in the range of 130-190 mg/dl and with no history of coronary heart disease were randomized into experimental and placebo groups. The experimental group received 1 gm of the extract for 90 days while the placebo received a similar amount of maltodextrin in addition to dietary and physical activity advice for the control of their blood lipids. Anthropometry, blood biochemistry, dietary and physical activity were assessed at baseline, day 45 and day 90. Results While body weight, serum LDL cholesterol and triglyceride levels decreased in both groups, there were no significant differences between the experimental and placebo group. Conclusions It is likely that the observed effects were as a result of the patients following the standard dietary and physical activity advice. At a dose of 1 gm/day, hibiscus sabdariffa leaf extract did not appear to have a blood lipid lowering effect. Trial Registration REFCTRI2009000472

  2. Análisis comparativo de compuestos fenólicos totales y actividad antioxidante de cuatro marcas de tisanas de Hibiscus sabdariffa (Malvaceae comercializadas en Costa Rica

    Directory of Open Access Journals (Sweden)

    Melissa Agüero

    2014-01-01

    Full Text Available Las tisanas de rosa de Jamaica (Hibiscus sabdariffa son consumidas en Costa Rica por su conocido efecto cardioprotector asociado a la presencia de compuestos fenólicos, principalmente antocianinas. Este trabajo estableció diferencias en el perfil de compuestos fenólicos y su actividad anti-radical entre diferentes comercializadores de tisanas de Hibiscus sabdariffa en Costa Rica. Se identificaron cualitativamente taninos, flavonoides y antocianinas. Se cuantificó la cantidad total de compuestos fenólicos por el método de Folin-Ciocalteu y se cuantificó la actividad antioxidante con el método de DPPH. Todas las marcas comerciales demostraron presencia de compuestos fenólicos, incluyendo antocianinas; sin embargo, se encontraron diferencias significativas en el contenido de compuestos fenólicos y actividad anti-radical, entre los diferentes productores de tisanas. Por ello, se concluye que en el mercado costarricense, el perfil en compuestos fenólicos de las tisanas en estudio varía de acuerdo con el productor.

  3. Actividad hipotrigliceridémica de un extracto de rosa de Jamaica (Hibiscus sabdariffa L. al administrarse antes y durante las comidas

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    Rodrigo Castañeda

    2015-11-01

    Full Text Available a elevación de los lípidos sanguíneos se ha convertido en un riesgo común de enfermedades cardiovasculares,en especial en el caso del colesterol y triglicéridos, también a problemas pancreáticos, de la córnea, bazo e hígado.Hibiscus sabdariffa L., es una especie medicinal de la familia Malvaceae, su efecto sobre la reducción de los lípidos séricos se ha mencionado en varios estudios. El objetivo del presente estudio consistió en evaluar la actividad de un extracto acuoso de los cálices de H. sabdariffa, para reducir los lípidos séricos en dos grupos de individuos, con valores de triglicéridos mayores de 150 mg/dL, al recibir una dosis de 15 mg de antocianinas totales al día, dividida en tres veces, por un periodo de dos meses para establecer su influencia, dependiendo del momento de su administración, un grupo recibiéndola antes de la comida y otro durante la misma. El extracto de H. sabdariffa mostró un efecto hipotrigliceridémico significativo (p = 0.034, al finalizar el tratamiento, únicamente al administrar el extracto antes de las comidas. Al mismo tiempo, no se observó alteración en los niveles de colesterol total, colesterol contenido en las lipoproteínas de baja densidad y colesterol contenido en las proteínas de alta densidad en ninguno de los dos grupos evaluados. Los resultados sugieren que los extractos acuosos de H. sabdariffa podrían ser utilizados para ejercer una acción en los triglicéridos plasmáticos, dependiente del consumo de alimentos, y del momento de administración.

  4. Hibiscus

    Science.gov (United States)

    ... also used to make jams, spices, soups, and sauces. The flowers are used to make medicine. Hibiscus ... mild to moderate high blood pressure. However, an analysis of results from various clinical studies suggests that ...

  5. Actividad hipotrigliceridémica de un extracto de rosa de Jamaica (Hibiscus sabdariffa L.) al administrarse antes y durante las comidas

    OpenAIRE

    Rodrigo Castañeda; Sully Margot Cruz; Armando Caceres

    2015-01-01

    a elevación de los lípidos sanguíneos se ha convertido en un riesgo común de enfermedades cardiovasculares,en especial en el caso del colesterol y triglicéridos, también a problemas pancreáticos, de la córnea, bazo e hígado.Hibiscus sabdariffa L., es una especie medicinal de la familia Malvaceae, su efecto sobre la reducción de los lípidos séricos se ha mencionado en varios estudios. El objetivo del presente estudio consistió en evaluar la actividad de un extracto acuoso de los cálices de H. ...

  6. INCLUSIÓN DE HIBISCUS SABDARIFFA EN ALIMENTOS EXPERIMENTALES PARA MACROBRACHIUM TENELLUM: EFECTOS EN LA PIGMENTACIÓN, CRECIMIENTO Y SUPERVIVENCIA.

    Directory of Open Access Journals (Sweden)

    Martín A. Aréchiga P.

    2015-01-01

    Full Text Available En la naturaleza, los pigmentos tienen la principal función de colorear; sin embargo, también tienen efectos sobre el estado de salud y desarrollo de los organismos. Los compuestos sintéticos dirigidos a incrementar la coloración en organismos con importancia comercial, representan entre el 15 al 20% del costo de la dieta. Por lo anterior, la investigación sobre pigmentos de origen natural (más baratos y menos tóxicos se ha incrementado sustancialmente en los últimos años. Las antocianinas pueden ser utilizadas como una alternativa para la pigmentación de organismos. En este estudio se utilizaron los cálices de Hibiscus sabdariffa, planta popular que acumula antocianinas que proporciona su característico color rojo. Langostinos Macrobrachium tenellum fueron alimentados con dietas que incluían cálices de H. sabdariffa (jamaica bajo los siguientes tratamientos: i pigmento extraído con agua, ii pigmento extraído con etanol acidificado [0.1 M HCl (v/v], y iii harina de cálices. El efecto pigmentante de las dietas experimentales se comparó con langostinos sometidos a una dieta control sin pigmentos añadidos y con langostinos extraídos del medio silvestre. Los langostinos alimentados con la dieta de inclusión de polvo de jamaica mostraron la mayor expresión de eritróforos (color rojo en el exoesqueleto del pleon (abdomen y ganancia en longitud y peso, después de 60 días. Estos resultados sugieren que H. sabdariffa puede ser utilizado como complemento alimenticio natural, puesto que tiene un efecto positivo en los parámetros de crecimiento y pigmentación.

  7. Comparative study of the anti-oxidant activity of the total polyphenols extracted from Hibiscus Sabdariffa L., Glycine max L. Merr., yellow tea and red wine through reaction with DPPH free radicals

    Directory of Open Access Journals (Sweden)

    T. Andzi Barhé

    2016-01-01

    Full Text Available The present study is part of the evaluation of extracts of Glycine max L. Merr and Hibiscus L. Sabdariffa as antioxidants. A comparative study was performed with extracts of yellow tea and commercial red wine, two foods known for their antioxidant activity. The method applied is free radical scavenging using the 1,1-diphenyl-2-picrylhydrazyl (DPPH°. The antioxidant properties were identified and measured by the determining the anti-radical activity reducing index, expressed in percentage % RSA (Radical Scavenger Activity, and by the determination of the colouring intensity (IC50. All results are compared to those of ascorbic acid as reference antioxidant. The results indicate the following order for the antioxidant power of the extracts tested. % RSA (tea > % RSA (Glycine max % > RSA (red wine % > RSA (Sabdariffa Hibiscus, and colouring intensities (IC50 ranging from 4.62 μM (ascorbic acid to 1.10 μM (Hibiscus sabdariffa correlated with their chemical structure and the content of phenolic compounds.

  8. Hibiscus sabdariffa Leaf Extract Inhibits Human Prostate Cancer Cell Invasion via Down-Regulation of Akt/NF-kB/MMP-9 Pathway

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    Chun-Tang Chiu

    2015-06-01

    Full Text Available Hibiscus sabdariffa leaf has been previously shown to possess hypoglycemic, hypolipidemic, and antioxidant effects, and induce tumor cell apoptosis. However, the molecular mechanisms involved in the anticancer activity of H. sabdariffa leaf extract (HLE are poorly understood. The object of the study was to examine the anti-invasive potential of HLE. First, HLE was demonstrated to be rich in polyphenols. The results of wound-healing assay and in vitro transwell assay revealed that HLE dose-dependently inhibited the migration and invasion of human prostate cancer LNCaP (lymph node carcinoma of the prostate cells under non-cytotoxic concentrations. Our results further showed that HLE exerted an inhibitory effect on the activity and expressions of matrix metalloproteinase-9 (MMP-9. The HLE-inhibited MMP-9 expression appeared to be a consequence of nuclear factor-kappaB (NF-κB inactivation because its DNA-binding activity was suppressed by HLE. Molecular data showed all these influences of HLE might be mediated via inhibition of protein kinase B (PKB, also known as Akt/NF-kB/MMP-9 cascade pathway, as demonstrated by the transfection of Akt1 overexpression vector. Finally, the inhibitory effect of HLE was proven by its inhibition on the growth of LNCaP cells and the expressions of metastasis-related molecular proteins in vivo. These findings suggested that the inhibition of MMP-9 expression by HLE may act through the suppression of the Akt/NF-kB signaling pathway, which in turn led to the reduced invasiveness of the cancer cells.

  9. PENENTUAN KUANTITATIF ZAT WARNA KARMOSIN,PONCEAU 4R DAN MERAH ALURA YANG DITAMBAHKAN DALAM MINUMAN AGREM (Hibiscus sabdariffa, Linn [Quantitative Determination of Carmoisine, Ponceau 4R and Allura Red Colouring Agents Added Into Softdrink Containing the Aqueous Extract of (Hibiscus sabdariffa, Linn

    Directory of Open Access Journals (Sweden)

    Embit Kartadarma

    2007-06-01

    Full Text Available The synthetic food-colouring agent is stiil commonly used in soft drink to enhance the colour of the food and to make foods more attractive, particularly for the drink containing natural colour. Addition of colour is legally permitted by the govermment, however, the product sometime contain the substance more than the permissible maximum dosage, and it may possibly cause iillhealth to the consumer. Preparation of soft drink containing the aqueous extract of Hibiscus sabdariffa fruits gave less intense colour due to the colour. The quantity of the synthetic food colour in soft drink must be determined quantitatively for food safety and the presence of natural colour in the products may affect the results. Determination of three synthetic colouring agents, carmoisine, ponceau 4R and allura red added into soft drink containing the aqueous extract of hisbicus sabdariffa was carried out. Result showed that the determination of such colouring agents can only be achivied after adjusting the pH up to 4.5 and the recovery of carmoisine, ponceau 4R and allura red were 99.8: 100.2 and 100.0%, with the accuracy of 0.1;0.3 and 0.1% and the precission of 0.1; 0.3 and 0.1% respectively.

  10. Biodiversity of aerobic endospore-forming bacterial species occurring in Yanyanku and Ikpiru, fermented seeds of Hibiscus sabdariffa used to produce food condiments in Benin.

    Science.gov (United States)

    Agbobatinkpo, Pélagie B; Thorsen, Line; Nielsen, Dennis S; Azokpota, Paulin; Akissoe, Noèl; Hounhouigan, Joseph D; Jakobsen, Mogens

    2013-05-15

    Yanyanku and Ikpiru made by the fermentation of Malcavene bean (Hibiscus sabdariffa) are used as functional additives for Parkia biglobosa seed fermentations in Benin. A total of 355 aerobic endospore-forming bacteria (AEFB) isolated from Yanyanku and Ikpiru produced in northern and southern Benin were identified using phenotypic and genotypic methods, including GTG5-PCR, M13-PCR, 16S rRNA, gyrA and gyrB gene sequencing. Generally, the same 5-6 species of the genus Bacillus predominated: Bacillus subtilis (17-41% of isolates), Bacillus cereus (8-39%), Bacillus amyloliquefaciens (9-22%), Bacillus licheniformis (3-26%), Bacillus safensis (8-19%) and Bacillus altitudinis (0-19%). Bacillus aryabhattai, Bacillus flexus, and Bacillus circulans (0-2%), and species of the genera Lysinibacillus (0-14%), Paenibacillus (0-13%), Brevibacillus (0-4%), and Aneurinibacillus (0-3%) occurred sporadically. The diarrheal toxin encoding genes cytK-1, cytK-2, hblA, hblC, and hblD were present in 0%, 91% 15%, 34% and 35% of B. cereus isolates, respectively. 9% of them harbored the emetic toxin genetic determinant, cesB. This study is the first to identify the AEFB of Yanyanku and Ikpiru to species level and perform a safety evaluation based on toxin gene detections. We further suggest, that the gyrA gene can be used for differentiating the closely related species Bacillus pumilus and B. safensis.

  11. A polyphenol extract of Hibiscus sabdariffa L. ameliorates acetaminophen-induced hepatic steatosis by attenuating the mitochondrial dysfunction in vivo and in vitro.

    Science.gov (United States)

    Lee, Chao-Hsin; Kuo, Chih-Yi; Wang, Chau-Jong; Wang, Chi-Ping; Lee, Yi-Ru; Hung, Chi-Nan; Lee, Huei-Jane

    2012-01-01

    Oxidative stress is the major contributor to acetaminophen (AAP)-caused liver damage. It promotes mitochondrial oxidative stress and collapses the mitochondrial membrane potential to cause cell death. We have previously shown that a polyphenol extract of Hibiscus sabdariffa L. (HPE) potentiated the antioxidative effect. We further examined in this study the possible mechanism of HPE against AAP-caused liver damage. BABL/c mice were orally fed with HPE (100, 200 or 300 mg/kg) for two weeks prior to an i.p. injection of 1000 mg/kg of AAP. The mice were decapitated 6 h after the AAP injection to collect the blood and liver for further determination. The results show that pretreating with HPE increased the level of glutathione (GSH), decreased the level of lipid peroxidation, and increased catalase activity in the liver. A histopathological evaluation shows that HPE could decrease AAP-induced liver sterosis accompanied by a decreased expression of AIF, Bax, Bid, and p-JNK in the liver. An in vitro assay revealed that HPE could reduce AAP-induced death of BABL/c normal liver cells (BNLs), reverse the lost mitochondrial potency and improve the antioxidative status, similarly to the results of the in vivo assay. We show in this study that HPE possessed the ability to protect the liver from AAP-caused injury. The protective mechanism might be regulated by decreasing oxidative stress and attenuating the mitochondrial dysfunction.

  12. Analysis of fatty acids from Hibiscus sabdariffa L.calyx by GC-MS%玫瑰茄花萼中脂肪酸成分的GC-MS分析

    Institute of Scientific and Technical Information of China (English)

    张冰涛; 赵婷; 邹艳敏; 邹烨; 郑大恒; 曲洪媛; 李峰; 仰榴青

    2012-01-01

    Objective; To analyze the fatty acids from Hibiscus sabdariffa L. Calyx by gas chromatogra-phy-mass spectrometry ( GC-MS). Methods; To extract oil from Hibiscus sabdariffa L. Calyx by soxhlet extraction method. After methyl esterification, the oil was analyzed by GC -MS. Results: The oil from Hibiscus sabdariffa L. Calyx was composed of seventeen fatty acids . The main fatty acids of the oil were linoleic acid (45.03%), oleic acid (23.62%), palmitic acid (22.91%), stearic acid (3.41%), (10E)10-nona-decenoic acid (1. 91%), palmitoleic acid (0. 84%), eicosanoic acid (0. 53%) and myristic acid (0.34%) , respectively. The content of unsaturated fatty acids was up to 71.40%. Conclusion; Unsatu-rated fatty acids, rich in Hibiscus sabdariffa L. Oil, are of high nutrition for human health.%目的:采用气相色谱-质谱联用技术(GC-MS)对玫瑰茄花萼中提取的油脂进行脂肪酸组成分析.方法:采用索氏提取法提取玫瑰茄花萼中的油脂,甲酯化后运用GC-MS联用技术进行分离和结构鉴定.结果:玫瑰茄花萼油脂中含有17种脂肪酸,其中主要的脂肪酸为亚油酸(45.03%)、油酸(23.62%)、棕榈酸(22.91%)、硬脂酸(3.41%)、(10E)-10-十九烯酸(1.91%)、棕榈油酸(0.84%)、花生酸(0.53%)和肉豆蔻酸(0.34%).其中不饱和脂肪酸含量高达71.40%以上.结论:玫瑰茄油脂中富含不饱和脂肪酸,具有较高的营养价值和保健功能.

  13. The protective effect of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2 against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes

    Directory of Open Access Journals (Sweden)

    Jamaludin Mohamed

    2013-10-01

    Full Text Available OBJECTIVES: The aim of this study was to investigate the protective effects of aqueous extracts of roselle (Hibiscus sabdariffa L. UKMR-2 against red blood cell (RBC membrane oxidative stress in rats with streptozotocin-induced diabetes. METHODS: Forty male Sprague-Dawley rats weighing 230-250 g were randomly divided into four groups (n = 10 rats each: control group (N, roselle-treated control group, diabetic group, and roselle-treated diabetic group. Roselle was administered by force-feeding with aqueous extracts of roselle (100 mg/kg body weight for 28 days. RESULTS: The results demonstrated that the malondialdehyde levels of the red blood cell membranes in the diabetic group were significantly higher than the levels in the roselle-treated control and roselle-treated diabetic groups. The protein carbonyl level was significantly higher in the roselle-treated diabetic group than in the roselle-treated control group but lower than that in the diabetic group. A significant increase in the red blood cell membrane superoxide dismutase enzyme was found in roselle-treated diabetic rats compared with roselle-treated control rats and diabetic rats. The total protein level of the red blood cell membrane, osmotic fragility, and red blood cell morphology were maintained. CONCLUSION: The present study demonstrates that aqueous extracts of roselle possess a protective effect against red blood cell membrane oxidative stress in rats with streptozotocin-induced diabetes. These data suggest that roselle can be used as a natural antioxidative supplement in the prevention of oxidative damage in diabetic patients.

  14. APOE and CETP TaqIB polymorphisms influence metabolic responses to Hibiscus sabdariffa L. and Gynostemma pentaphyllum Makino tea consumption in hypercholesterolemic subjects.

    Science.gov (United States)

    Jeenduang, Nutjaree; Sangkaew, Boonnisa; Chantaracha, Pacharee; Chanchareonsri, Sirada; Plyduang, Thunyaluk; Thitdee, Wanida; Samae, Cathaleeya; Pitumanon, Wacharaporn

    2017-03-01

    Hibiscus sabdariffa L. (HS) and Gynostemma pentaphyllum Makino (GP) have been used as traditional medicines to treat diabetes and hypercholesterolemia. Nevertheless, there is interindividual variation in the metabolic responses to HS and GP consumption. This may be due to genetic factors. The aim of this study was to investigate the effects of HS and GP tea consumption on anthropometric data, fasting blood glucose (FBG), and lipid concentrations in hypercholesterolemia subjects with different genotypes of the APOE and CETP TaqIB polymorphisms. Forty-eight subjects with hypercholesterolemia were given either HS or GP tea for 30 days. Anthropometric and biochemical variables were determined, and APOE and CETP TaqIB polymorphisms were analyzed using the polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP). E4 (p=0.008) and homozygous B1B1 (p=0.010) carriers had significantly decreased HDL-C concentrations after HS consumption; in addition, B2 carriers who consumed HS showed significantly decreased triglyceride (TG) concentrations (p=0.039). Regarding GP consumption, non-E4 carriers had significantly decreased HDL-C (p=0.009) and FBG (p=0.042) concentrations. Furthermore, B2 carriers had significantly decreased total cholesterol (TC) (p=0.045), HDL-C (p=0.004), and FBG (p=0.026) concentrations. HS consumption may have beneficial effects with respect to TG concentrations in the B2 carriers, but it may adversely affect HDL-C concentrations in homozygous B1B1 and E4 carriers. In contrast, GP consumption may have favorable effects on TC and FBG concentrations but not on HDL-C concentrations for B2 and/or non-E4 carriers.

  15. Detection of somaclonal variation in micropropagated Hibiscus ...

    African Journals Online (AJOL)

    User

    tissue culture techniques offers several advantages such as production of disease- free plants ..... Current Issues in Plant Molecular and Cellular Biology, pp 247-252. (Kluwer ... Natural cross-pollination in Roselle, Hibiscus sabdariffa L.

  16. Actividad antioxidante de extractos de cálices deshidratados de 64 variedades de jamaica (hibiscus sabdariffa l. en función de fenólicos y antocianinas totales

    Directory of Open Access Journals (Sweden)

    Raquel Enedina Medina-Carrillo

    2013-01-01

    Full Text Available La jamaica (Hibiscus sabdariffa L. es una planta rica en compuestos bioactivos, tales como polifenoles, flavonoides, vitaminas, fibra y anto- cianinas. Se comercializa principalmente en forma de cálices deshidratados con los que se preparan infusiones acuosas que promueven efectos benéficos en la salud. El objetivo de este trabajo fue evaluar la actividad antioxidante (actividad antirradical y capacidad de reducción de iones hierro de los extractos acuosos de 64 variedades de jamaica, la concentración de compuestos bioactivos (compuestos fenólicos totales y antocianinas monoméricas totales y la correlación que existe entre ellos. Se encontró que la actividad antioxidante de los extractos está prin- cipalmente correlacionada con la concentración de antocianinas monoméricas totales y en menor medida con los compuestos fenólicos totales.

  17. Effects of consuming diets containing Agave tequilana dietary fibre and jamaica calyces on body weight gain and redox status in hypercholesterolemic rats.

    Science.gov (United States)

    Sáyago-Ayerdi, Sonia G; Mateos, Raquel; Ortiz-Basurto, Rosa I; Largo, Carlota; Serrano, José; Granado-Serrano, Ana Belén; Sarriá, Beatriz; Bravo, Laura; Tabernero, María

    2014-04-01

    Dietary fibre (DF) obtained from Agave tequilana, which is rich in fructans and insoluble DF, and jamaica calyces (Hibiscus sabdariffa), which is rich in DF and phenolic compounds, were assessed as new potential functional ingredients using the hypercholesterolemic animal model. Wistar rats (200-250 g) were divided into 3 groups (n=8) and fed with cholesterol-rich diets supplemented with cellulose (CC, control), agave DF (ADF) or ADF with jamaica calyces (ADF-JC). After consuming the test diets for 5 weeks, weight gain in the ADF-JC group was significantly lower than in the other groups. The ADF and ADF-JC groups had a reduced concentration of cholesterol transporters in the caecum tissue, although no changes were observed in the plasma lipid profile. Both treatments improved the redox status by reducing the malondialdehyde serum levels and protein oxidative damage, compared to the CC group. DF from A. tequilana alone, or in combination with jamaica calyces, shows promising potential as a bioactive ingredient.

  18. Ekspresi COX-2 dan Jumlah Neutrofil Fase Inflamasi pada Proses Penyembuhan Luka Setelah Pemberian Sistemik Ekstrak Etanolik Rosela (Hibiscus sabdariffa (studi in vivo pada Tikus Wistar

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    Endah Kusumastuti

    2014-06-01

    sebagai bahan anti-inflamasi.  Expression of COX-2 and The Number of Neutrophil in Inflammation stage of Wound Healing Process after Systemic Administration of Ethanolic Extract Rosela. Inflammation is an initial stage of body’s natural response to tissue damage.The use  empirically plants often used for traditional medicine because it is easily found in the community and fewer side effects. Flavanoid presence of roselle (Hibiscus sabdariffa is thought to have anti inflammatory effects. This study aimed to know the effect of systemic administration of Roselle ethanolic extract toward COX-2 expression and neutrophils number in the inflammatory phase of wound healing processes. Roselle was obtained from plantations in Bulusari hamlet, Tarokan, Kediri, EastJava. Making roselle extract was performed in LPPT unit 1 UGM Yogyakarta by percolation ways. Wistar rats were given a total of 36 injuries with ɵ 3 mm punch biopsy of the buccal mucosa. Subjects were divided into three groups, each group of 12 rats. The division consists of the negative control group (saline, positive control (ibuprofen 20 mg/kg and treatment (roselle extract 500 mg/kg. Giving drink suitable group once daily for four days. On day 1, the 2nd, 3rd and fourth rats were sacrificed, and mucosal tissue injury was made histological preparat. Hematoxylin eosin staining (HE was performed to observe the number of neutrophils. COX-2 expression was found in preparations for immunohistochemical staining using rabbit polyclonal COX-2 antibody (Lab Vision, USA. The number of neutrophils and expression of COX-2 is calculated under a light microscope data were analyzed using Two-way ANOVA and LSD. The results showed that the expression of COX-2 and neutrophil number were least in the treatment group compared to the control. Clinical observation on day four also appears around the wound has completely closed the subject after administration of roselle drink. It was concluded that the ethanolic extract of roselle can

  19. Evaluation of the Potential Nephroprotective and Antimicrobial Effect of Camellia sinensis Leaves versus Hibiscus sabdariffa (In Vivo and In Vitro Studies

    Directory of Open Access Journals (Sweden)

    Doa’a Anwar Ibrahim

    2014-01-01

    Full Text Available Green tea and hibiscus are widely consumed as traditional beverages in Yemen and some regional countries. They are relatively cheap and the belief is that they improve health state and cure many diseases. The aim of this study was to evaluate the potential protective and antibacterial activity of these two famous plants in vitro through measuring their antibacterial activity and in vivo through measuring nonenzymatic kidney markers dysfunction after induction of nephrotoxicity by gentamicin. Gram positive bacteria like MRSA (methicillin resistant Staphylococcus aureus were isolated from hospitalized patients’ different sources (pus and wound and Gram negative bacteria including E. coli and P. aeruginosa were used in vitro study. In addition, the efficacy of these plants was assessed in vivo through measuring nonenzymatic kidney markers including S. creatinine and S. urea. Green tea was shown antimicrobial activity against MRSA with inhibition zone 19.67 ± 0.33 mm and MIC 1.25 ± 0.00 mg/mL compared with standard reference (vancomycin 18.00 ± 0.00 mg/mL. Hibiscus did not exhibit a similar effect. Both Hibiscus- and green tea-treated groups had nephroprotective effects as they reduced the elevation in nonenzymatic kidney markers. We conclude that green tea has dual effects: antimicrobial and nephroprotective.

  20. Effets comparés des cendres de Chromolaena odorata (L. King R.M. & H.E. Rob et d'un engrais minéral soluble dans l'eau (NPK 15 15 15 sur la croissance et le rendement de l'oseille de Guinée (Hibiscus sabdariffa L.

    Directory of Open Access Journals (Sweden)

    Ognalaga, M.

    2016-01-01

    Full Text Available Comparative Effects of Chromolaena odorata L. King R.M. & H.E. Rob Ashes and a Soluble Mineral Fertilizer (NPK 15 15 15 on the Growth and Yield of Guinea Sorrel (Hibiscus sabdariffa L.. The low levels of nutrients observed in the lateritic soils of Gabon are a concern. A trial conducted in vegetation pots was laid out in a Fisher randomized complete block design with 20 treatments and 3 replicates. The experiment aimed at comparing the growth and yield of Hibiscus sabdariffa L. under different fertilization modalities: a water soluble mineral fertilizer (NPK 15-15-15 added at four doses (0-0-0; 16-7-13; 19-8-16; 23-10-19 mg.kg-1 soil; Chromolaena odorata ashes at 0; 500; 667; 834; 1000 mg.kg-1 soil; and comparing the interactions between ash doses (C and NPK doses (M. The C. odorata ash supply increased significantly the diameter of the stems, the height of the stems and the leaf area up to a dose of 667 mg.kg-1 soil. Among the compared doses, the one of 23-10-19 mg.kg-1 soil (M3 induced the highest effect on the plant growth parameters. The highest growth (167±68 g. fresh vegetal matter per pot was obtained with the combination of 667 mg.kg-1 ashes at and 19-8-16 mg.kg-1 NPK (C2M2.

  1. Utilization of dried roselle calyces extract in fruit juice processing

    OpenAIRE

    Kilima, Beatrice Mgaya

    2014-01-01

    There has been an increased consumption of fruit juice as consumers have become aware of the nutritional and health benefits of fruit juices. This increased consumption goes together with the growth of varieties of fruit juices and beverages offered for sale. Among these products is roselle (Hibiscus sabdariffa L.) juice, or drink, which is a good source of anthocyanins, vitamins (thiamine, riboflavin, niacin, ascorbic acid) and minerals (Ca, P, Fe). In addition, roselle is also used as flavo...

  2. 玫瑰茄花萼乙醇提取物的抗痛觉敏感、抗炎及止泻作用的实验研究%Antinociceptive, anti-inflammatory and antidiarrheal activities of ethanolic calyx extract of Hibiscus sabdariffa Linn.(Malvaceae) in mice

    Institute of Scientific and Technical Information of China (English)

    Md. Khadem Ali; Ayesha Ashraf; Nripendra Nath Biswas; Utpal Kumar Karmakar; Shamima Afroz

    2011-01-01

    Objective:To evaluate the antinociceptive, anti-inflammatory and antidiarrheal activities of the ethanolic calyx extract of Hibiscus sabdariffa Linn, in mice.Methods: In the present study, the dried calyxes of H.sabdariffa were subjected to extraction with 95% ethanol and the extract was used to investigate the possible activities. Antinociceptive activity of the extract was evaluated by using the acetic acid-induced writhing test. The anti-inflammatory effect of the extract was tested by using the xylene-induced ear edema model mice. Castor oil-induced diarrheal model mice were used to evaluate the antidiarrheal activity of the extract.Results: In acetic acid-induced writhing test, the extract produced inhibited writhing in mice siginificantly compared with the blank control (P<0. 01). The extract showed significant inhibition of ear edema formation in xylene-induced ear edema model mice in a dose-related manner compared with the blank control (P<0. 01). The extract demonstrated a significant antidiarrheal activity against castor oil-induced diarrheal in mice in which it decreased the frequency of defecation and increased the mean latent period at the doses of 250 and 500 mg/kg body weight (P<0.01).Conclusion; The above mentioned findings indicate that the calyx extract of H.sabdariffa possesses significant antinociceptive, anti-inflammatory and antidiarrheal activities that support its uses in traditional medicine.%目的:研究玫瑰茄(Hibiscus sabdariffa)花萼的乙醇提取物的抗痛觉敏感、抗炎及止泻作用.方法:使用95%乙醇提取玫瑰茄花萼干品用于测定其功效.用小鼠扭体实验检测其抗痛觉敏感作用,二甲苯致耳水肿模型小鼠检测其抗炎症作用,蓖麻油致腹泻模型小鼠检测其止泻作用.结果:在乙酸致小鼠扭体实验中,玫瑰茄花萼的乙醇提取物对小鼠扭体的抑制与空白对照组相比差异有统计学意义(P%0.01),对二甲苯致耳水肿模型小鼠的耳水肿的抑制

  3. (Hibiscus sabdariffa L.) roots by differential display

    African Journals Online (AJOL)

    aghomotsegin

    2014-12-31

    Dec 31, 2014 ... PCR, polymerase chain reaction. ... A DDRT-PCR reaction was performed in a 25 µL volume containing 0.5 µL of Taq ... V for 90 min and analyzed under UV light. .... less Na+ and Cl- and showed improved photosynthesis in.

  4. “SOBO” (Hibiscus Sabdariffa-Ros

    African Journals Online (AJOL)

    KAYCEE

    fleshly, and cup shaped calyxes plant. ... used as vegetables, refreshing drinks source of oils and food preservatives (Wong ... good health care services. ... Table 2: Percentage distribution of respondents' based on sources of information ... vitamin and mineral salt deficiency diseases such as scurvy, rickets, skin lesions,.

  5. Calyceal Diverticula: A Comprehensive Review

    Science.gov (United States)

    Waingankar, Nikhil; Hayek, Samih; Smith, Arthur D; Okeke, Zeph

    2014-01-01

    Calyceal diverticula are rare outpouchings of the upper collecting system that likely have a congenital origin. Stones can be found in up to 50% of calyceal diverticula, although, over the combined reported series, 96% of patients presented with stones. Diagnosis is best made by intravenous urography or computed tomography urogram. Shock wave lithotripsy (SWL) is an option for first-line therapy in patients with stone-bearing diverticula that have radiologically patent necks in mid- to upper-pole diverticula and small stone burdens. Stone-free rates are the lowest with SWL, although patients report being asymptomatic following therapy in up to 75% of cases with extended follow-up. Ureteroscopy (URS) is best suited for management of anteriorly located mid- to upperpole diverticular stones. Drawbacks to URS include difficulty in identifying the ostium and low rate of obliteration. Percutaneous management is best used in posteriorly located mid- to lower-pole stones, and offers the ability to directly ablate the diverticulum. Percutaneous nephrolithotomy remains effective in the management of upperpole diverticula, but carries the risk of pulmonary complications unless subcostal access strategies such as triangulation or renal displacement are used. Laparoscopic surgery provides definitive management, but should be reserved for cases with large stones in anteriorly located diverticula with thin overlying parenchyma, and cases that are refractory to other treatment. This article reviews the current theories on the pathogenesis of calyceal diverticula. The current classification is examined in addition to the current diagnostic methods. Here we summarize an extensive review of the literature on the outcomes of the different treatment approaches. PMID:24791153

  6. The Aqueous Calyx Extract of Hibiscus sabdariffa Lowers Blood ...

    African Journals Online (AJOL)

    The cold pressor test (CPT) and handgrip exercise (HGE) were performed in ... The blood pressure (BP) and heart rate (HR) responses were measured digitally. ... In the presence of HS, CPT-induced changes (ΔMAP=10.1±1.7mmHg; ΔHR= ...

  7. Roselle (Hibiscus sabdariffa L.) seeds as unconventional nutritional ...

    African Journals Online (AJOL)

    mohamhed

    2012-05-22

    May 22, 2012 ... The composition of roselle seed from oil, protein, ash, fiber, fatty acids and amino acids was determined and ... (Robert, 1996); in Sudan, it is used for edible oil production and ..... Experimental Design and Analysis in Scientific.

  8. Response of Roselle (Hibiscus sabdariffa L.) to Farmyard Manure ...

    African Journals Online (AJOL)

    acer

    t/ha) and four levels of nitrogen fertiliser (0, 25, 50 and 75 kg N/ha) laid out in a randomized complete block design ... Thus, 2.5 t/ha manure and 50 kg N/ha were considered adequate for calyx production in ..... Soc. Proceeding. 26: 245-254.

  9. Fermentation Potentials of Citrus Limon and Hibiscus Sabdariffa ...

    African Journals Online (AJOL)

    MASANAWA

    The standardized fermentation showed significant change in pH (α=0.05>p 0.00). The FT-IR ... Stems are reddish in color and up to 3.5m tall. Leaves are dark ... Leaf juice is used to treat conjunctivitis. ..... agricultural soil". Journal of Basic.

  10. The Aqueous Calyx Extract of Hibiscus sabdariffa Lowers Blood ...

    African Journals Online (AJOL)

    olayemitoyin

    *Address for correspondence: mojiminiyi@yahoo.co.uk. Manuscript Accepted: .... digital sphygmomanometer (HuBDIC Co. Ltd., .... data from our laboratory suggest that responses to .... composition, protein quality and health benefits. Food ...

  11. Anatomical factors predicting lower calyceal stone clearance after extracorporeal shockwave lithotripsy

    Directory of Open Access Journals (Sweden)

    M. Khan

    2016-06-01

    Conclusions: This study shows that lower infundibular length and width are significant anatomical factors in determining stone clearance following ESWL treatment of lower calyceal stones and these should be assessed before planning the treatment for lower calyceal stones.

  12. The imaging appearances of calyceal diverticula complicated by uroliathasis.

    LENUS (Irish Health Repository)

    Stunell, H

    2012-02-01

    The presence of diverticula arising from the calyceal system is a relatively uncommon urological problem, occurring with an incidence of 2.1-4.5 per 1000 intravenous urogram (IVU) examinations. While the incidence of calyceal diverticula is low, the frequency of stone formation within them is high. We describe the aetiology and clinical presentation and describe the role of imaging with ultrasound, intravenous and retrograde pyelography and CT in diagnosis and planning treatment. We also describe the potential of fluid-sensitive magnetic resonance imaging techniques as a radiation-free alternative to the use of more conventional modalities, such as intravenous urography and retrograde pyelography, in delineating the anatomy of calyceal diverticula before surgical and radiological intervention especially in young patients and pregnant women.

  13. Studies on mutation breeding of hibiscus Syriacus

    Energy Technology Data Exchange (ETDEWEB)

    Song, Heui Sub; Lee, Ki Woon; Im, Yong Taek [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1994-12-01

    Hibiscus(Hibiscus syracuse L.) has been know as a national flower of Korea science old times. Although there are some ancient records that the Hibiscus had been planted in large quantities in Korea, Japanese had dug out all the good plants of Hibiscus in this country during their colonial period. But Hibiscus has such a characteristics of self-incompatibility that all the plants exist as a hybrid naturally and have heterogeneous genes. Therefore many good characters can be taken out from the surviving plants. Many domestic 78 varieties of Hibiscus syracuse were collected and propagated 26 varieties cuttings. Radiosensitivity of gamma-ray irradiated Hibiscus syracuse were investigated the germination rate, survival rate, plant height was with the increase of 4 kR better than control. The radiation doses of 10-12 kR are recommended for mutation breeding of Hibiscus. 6 figs, 11 tabs, 41 refs. (Author).

  14. Fatty Acid Composition of Hibiscus trionum L. (Malvaceae

    Directory of Open Access Journals (Sweden)

    Ceyda Sibel Kılıç

    2011-01-01

    Full Text Available The genus Hibiscus plants have different uses, some are used as foods (H. esculenta L., and some species as remedy in traditional medicine (H. sabdariffa L. as well as a colorant for herbal teas. The only species that grows naturally in Turkey is H. trionum L. The plant especially infests soy and corn fields, and therefore it is considered to be a noxious weed. The plant is also found to be a host for Potato Virus Y (PVY and Tomato Spotted Wilt Virus (TSWV. Infestation of the crop fields by this plant shows that it germinates easily. Since it produces many seeds, distribution of the plant is also quiet easy. Though, seed oil yield is low (4.7% since linoleic acid composition of the seed oil is quiet high (67,5%, it can still be used as a source of unsaturated fatty acids. The other major fatty acids are palmitic, oleic and stearic acids in the studied seed oil .

  15. Preterm Delivery in the Setting of Left Calyceal Rupture

    OpenAIRE

    2015-01-01

    Spontaneous rupture of the renal collecting system is a rare but serious complication of pregnancy. We report a case of nontraumatic left renal calyceal rupture in a pregnancy which ultimately progressed to preterm delivery. A 29-year-old primigravida with a remote history of urolithiasis presented with left flank pain, suprapubic pain, and signs of preterm labor at 33 weeks of gestation. The patient was believed to have urolithiasis, although initial renal ultrasound failed to demonstrate de...

  16. Microwave Assisted Extraction of Defatted Roselle (Hibiscus sabdariffa L. Seed at Subcritical Conditions with Statistical Analysis

    Directory of Open Access Journals (Sweden)

    N. I. Yusoff

    2017-01-01

    Full Text Available Roselle seeds are the waste product of roselle processing, but they are now labeled as a polyphenol source with great herbal quality. In this work, polyphenols were extracted using ethanol-water (70% (v/v in a closed vessel under microwave irradiation. The main objective was to determine the optimal parameters statistically. The influence of extraction time (4–10 min, microwave power (100–300 W, and solvent/solid ratio (25–100 mL/g was studied. The total phenolic and flavonoids content were determined using Folin-Ciocalteu and aluminum chloride methods, respectively. Without temperature control, the subcritical conditions could occur and the highest flavonoid content (14.4251 mg QE/g was achieved at 158°C and 16.4 bar. Although the optimum MAE conditions (10 min, 300 W, and 97.7178 mL/g resulted in the highest yield (65.0367% and phenolic content (18.2244 mg GAE/g, low flavonoids content (6.4524 mg QE/g was unexpectedly obtained due to degradation at 163°C.

  17. Antioxidant Effect and Water-Holding Capacity of Roselle (Hibiscus sabdariffa L. Seed Protein Hydrolysates

    Directory of Open Access Journals (Sweden)

    Fatoumata Tounkara

    2013-06-01

    Full Text Available The aim of this study was to investigate the effect of in-vitro pepsin and pancreatin digestion of proteins extracted from Roselle seed on the production of bioactive peptides. Defatted Roselle seed flour was used to extract different protein fractions namely globulin, albumin and glutelin. The proteins were digested using pepsin (1 h followed by pancreatin (1 h in order to produce hydrolysates with good antioxidant activity. The prepared hydrolysates were as effective as antioxidants in model systems, in scavenging of free radicals and acting as reducing agents. This effect was concentration-dependent and was also influenced by the type of protein fraction. The albumin fraction hydrolysates prepared showed the highest antioxidant activity followed by Glutelin and Globulin hydrolysates respectively (Albumin hydrolysates>Glutelin hydrolysates>Globulin hydrolysates. All of the prepared hydrolysates were also found to be effective in enhancing water-holding capacity and cooking yield in a meat model system. Albumin hydrolysates showed the highest improved meat cooking ability followed by Glutelin and Globulin respectively (Albumin hydrolysates>Glutelin hydrolysates>Globulin hydrolysates. The molecular weight distribution analysis of the hydrolysates was determined and most of the peptides were found between 1000 Da and below. The study findings suggest that Roselle seed protein hydrolysates can be applied as functional food ingredients and that their composition determines their functional properties thus their potential application in the food and feed industries.

  18. Drying of Rosella (Hibiscus sabdariffa Flower Petals using Solar Dryer with Double Glass Cover Collector

    Directory of Open Access Journals (Sweden)

    Tjukup Marnoto

    2014-10-01

    Full Text Available Chemical ingredients in rosella petals are very beneficial for health. Rosella petals needed to be drained for storage and packing purpose. The traditional drying takes 5 days and less healthy. Solar dryer technology can speed up the drying process and protect materials from dust contamination. Solar dryer with double glass covered collector has been designed and made for drying of agricultural products such as rosella flowers. Rosella petals as much as 2300 grams with initial moisture content of 90.84 % be dried with this dryer until the moisture content of 7.67 % takes only 2 days, although the weather was less sunny . The temperature in the drying chamber was not more than 50° C, so it was good for drying groceries, not damaging chemical ingredients. The relative humidity in the space dryeris was about 40 % and it was still relative low. Drying rate and drying performance was expressed by the efficiency and Specific Moisture Evaporation Rate ( SMER were influenced by water content of the dried material and weather. Daily efficiency at the first and the second day: 14.931 % and 5.78%, while the daily SMER on the first and the second day: 0.222 and 0.0256 ( kg / kWh .

  19. Preterm Delivery in the Setting of Left Calyceal Rupture

    Directory of Open Access Journals (Sweden)

    Brent Hanson

    2015-01-01

    Full Text Available Spontaneous rupture of the renal collecting system is a rare but serious complication of pregnancy. We report a case of nontraumatic left renal calyceal rupture in a pregnancy which ultimately progressed to preterm delivery. A 29-year-old primigravida with a remote history of urolithiasis presented with left flank pain, suprapubic pain, and signs of preterm labor at 33 weeks of gestation. The patient was believed to have urolithiasis, although initial renal ultrasound failed to demonstrate definitive calculi. After a temporary improvement in flank pain with medication, the patient experienced acute worsening of her left flank pain. Urology was consulted and further imaging was obtained. Magnetic resonance imaging (MRI was consistent with bilateral hydronephrosis and rupture of the left renal calyx. Given the patient’s worsening pain in the setting of left calyceal rupture, the urology team planned for placement of a left ureteral stent. However, before the patient could receive her stent, she progressed to active labor and delivered a viable female infant vaginally. Following delivery, the patient’s flank pain resolved rapidly and spontaneously, so no surgical intervention was performed. A summary of the literature and the details of this specific clinical situation are provided.

  20. Preterm Delivery in the Setting of Left Calyceal Rupture

    Science.gov (United States)

    Hanson, Brent; Tabbarah, Rami

    2015-01-01

    Spontaneous rupture of the renal collecting system is a rare but serious complication of pregnancy. We report a case of nontraumatic left renal calyceal rupture in a pregnancy which ultimately progressed to preterm delivery. A 29-year-old primigravida with a remote history of urolithiasis presented with left flank pain, suprapubic pain, and signs of preterm labor at 33 weeks of gestation. The patient was believed to have urolithiasis, although initial renal ultrasound failed to demonstrate definitive calculi. After a temporary improvement in flank pain with medication, the patient experienced acute worsening of her left flank pain. Urology was consulted and further imaging was obtained. Magnetic resonance imaging (MRI) was consistent with bilateral hydronephrosis and rupture of the left renal calyx. Given the patient's worsening pain in the setting of left calyceal rupture, the urology team planned for placement of a left ureteral stent. However, before the patient could receive her stent, she progressed to active labor and delivered a viable female infant vaginally. Following delivery, the patient's flank pain resolved rapidly and spontaneously, so no surgical intervention was performed. A summary of the literature and the details of this specific clinical situation are provided. PMID:26483981

  1. A neural network approach to discrimination between defects and calyces in oranges

    Directory of Open Access Journals (Sweden)

    Salvatore Ingrassia

    1993-11-01

    Full Text Available The problem of automatic discrimination among pictures concerning either defects or calyces in oranges is approached. The method here proposed is based on a statistical analysis of the grey-levels and the shape of calyces in the pictures. Some suitable statistical indices are considered and the discriminant function is designed by means of a neural network on the basis of a suitable vector representation of the images. Numerical experiments give 5 misclassifications in a set of 52 images, where only three defects have been classified as calyces.

  2. Studies on mutation breeding of hibiscus syriacuse

    Energy Technology Data Exchange (ETDEWEB)

    Song, Hee Sub; Kim, Jin Kyu; Lee, Ki Un; Lim, Yong Taek [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    1995-12-01

    Hibiscus has been known as a national flower of Korea. Hibiscus has ahch a characteristic of self-incompatibility that all the plants exist as natural hybrids and have heterogeneous genes. Thirth two domestic varieties were propagated. Radiosensitivity of H. syriacus irradiated with gamma ray was investigated in plant cuttings. The plant height was reduced by 45 percent in 5 kR irradiated group compared to control group. The radiation dose of 5 kR could be rrecommended for mutation breeding of Hibiscus cuttings. Promising mutant lines were selected form the varieties of Hwarang Wolsan 176, I1pyondansim and Emille. 6 tabs., 2 figs., 13 refs., 4 ills. (Author).

  3. Percutaneous balloon dilatation of stenotic calyceal diverticular infundibula in patients with recurrent urinary tract infections.

    LENUS (Irish Health Repository)

    Keeling, Aoife N

    2011-02-01

    Renal calyceal diverticula are usually detected as incidental findings on intravenous pyelograms (IVPs) and rarely manifest any clinical signs or symptoms. However, they can interfere with patient\\'s quality of life in a number of instances causing pain, recurrent urinary tract infections (UTIs), abscess, systemic sepsis and calculus formation. The purpose of this study was to review the clinical indications, procedure technique and clinical outcome in all patients referred to Interventional Radiology for the percutaneous management of renal calyceal diverticula.

  4. Lower Pole Calyceal Stone and Lithotripsy are Issues with Clearance Fact or Reality?

    Directory of Open Access Journals (Sweden)

    M. Hammad Ather

    2013-12-01

    Full Text Available The lower pole calyceal (LPC stone continues to be an enigma. The complex anatomy of the lower pole collecting system, along with other factors like acute pelvi calyceal angle and narrow and long infundibulum, are some of the complicating factors affecting stone clearance. There have been many studies assessing the impact of collecting system anatomy and most conclude that the complex anatomy of the lower pole collecting system does impact the overall stone-free rate.

  5. Alpha adrenergic receptors in renal pelvis and calyces: can rat models be used?

    Directory of Open Access Journals (Sweden)

    Osman Raif Karabacakb

    2014-10-01

    Full Text Available We aimed, in this study, to determine the distribution of α-1 AR subtypes in rat and human pelvis and calyces, and to evaluate, by comparing these two species, the possibility of rats to be used as models for humans. Twenty patients with renal carcinoma were included into the study. The patients underwent radical nephrectomy for renal cell carcinoma (RCC. After nephrectomy, specimens were evaluated and excisional biopsies from healthy pelvis and calyces tissues were performed. When pathology confirmed the non-invasion of RCC, specimen was included into the study. A total of 7 adult Wistar Albino (250-300 g female rats were used in this study. Specimens included renal pelvis and calyces. All specimens were evaluated under light microscope histopathologically. The concentrations of the receptor densities did not differ between the two groups. With the demonstration of the α receptors in rat kidneys and calyces, many receptor-based studies concerning both humans and rats can take place. Novel medication targeting these subtypes -in this matter α1A and α1D for renal pelvis and calyces- may be helpful for expulsive therapy and/or pain relief. With the demonstration of similar receptor densities between human and rat tissues, rat model may be useful for α-receptor trials for renal pelvis and calyces.

  6. Can infundibular height predict the clearance of lower pole calyceal stone after extracorporeal shockwave lithotripsy?

    Directory of Open Access Journals (Sweden)

    M. Arzoz-Fabregas

    2009-04-01

    Full Text Available PURPOSE: To evaluate the efficacy of extracorporeal shock wave lithotripsy (SWL on lower calyceal calculi in relation to the renal anatomical factors and determine which of these factors can be used to select patients who will benefit from SWL. MATERIAL AND METHODS: We analyzed retrospectively 78 patients with single radiopaque lower calyceal stones treated with SWL. The patients were evaluated 3 months after lithotripsy with a simple abdominal X-ray and a kidney ultrasound scan. The success of the treatment, removal of all fragments, was correlated with renal anatomical factors measured in the pre-treatment intravenous urography: infundibulopelvic angle, lower infundibulum width, lower infundibulum length, ratio length/width, infundibulum height, and number of minor calyces in the lower calyceal group. RESULTS: Three months after SWL treatment, 39 patients were stone-free (NR group and 39 had residual fragments (R group. Both groups presented no differences in relation to infundibulopelvic angle, width and length of the lower calyceal infundibulum, length/width ratio of the lower infundibulum or number of lower calyces. Height of the infundibulum, described as the distance between the line passing through the lowest part of the calyx containing the calculus and the highest point of the lower lip of renal pelvis, was the only parameter in which significant differences (p = 0.002 were found between the NR and R groups. CONCLUSIONS: Lower Infundibular height could be a good measurement tool for deciding which patients with lower calyceal lithiasis would benefit from SWL treatment. Height of less than 22 mm suggests a good outcome from lithotripsy.

  7. Percutaneous balloon dilatation of stenotic calyceal diverticular infundibula in patients with recurrent urinary tract infections.

    LENUS (Irish Health Repository)

    Keeling, Aoife N

    2012-02-01

    AIM: Renal calyceal diverticula are usually detected as incidental findings on intravenous pyelograms (IVPs) and rarely manifest any clinical signs or symptoms. However, they can interfere with patient\\'s quality of life in a number of instances causing pain, recurrent urinary tract infections (UTIs), abscess, systemic sepsis and calculus formation. The purpose of this study was to review the clinical indications, procedure technique and clinical outcome in all patients referred to Interventional Radiology for the percutaneous management of renal calyceal diverticula. MATERIALS AND METHODS: A retrospective review of all patients treated with percutaneous balloon dilatation of calyceal diverticular infundibula in the Interventional Radiology Department over a 10-year period was performed. Data collected included clinical details, laboratory indices, IVP and CT findings, procedure details and clinical outcome. RESULTS: A total of three patients were treated over the course of the study period. All patients were young females who presented as a result of recurrent urinary tract infections. The calyceal diverticula were diagnosed incidentally on routine IVPs, with CT eloquently confirming the plain film findings in two cases. Technical procedure success was achieved in all cases, avoiding surgical intervention. Clinical follow-up revealed no further UTIs following dilatation. CONCLUSION: Fluoroscopic guided percutaneous balloon dilatation of renal calyceal diverticular infundibula following direct diverticular puncture is a safe and well tolerated method to reduce UTI and potentially avoid future stone formation.

  8. Percutaneous balloon dilatation of stenotic calyceal diverticular infundibula in patients with recurrent urinary tract infections

    Energy Technology Data Exchange (ETDEWEB)

    Keeling, Aoife N. [Department of Academic Radiology, Beaumont Hospital, Beaumont Road, Dublin 9 (Ireland); Wang, Tim T. [Department of Biosurgery and Surgical Technology, Surgical Epidemiology and Quality Unit, Imperial College London, St. Mary' s Hospital, London W2 1NY (United Kingdom); Lee, Michael J., E-mail: mlee@rcsi.ie [Department of Academic Radiology, Beaumont Hospital, Beaumont Road, Dublin 9 (Ireland)

    2011-02-15

    Aim: Renal calyceal diverticula are usually detected as incidental findings on intravenous pyelograms (IVPs) and rarely manifest any clinical signs or symptoms. However, they can interfere with patient's quality of life in a number of instances causing pain, recurrent urinary tract infections (UTIs), abscess, systemic sepsis and calculus formation. The purpose of this study was to review the clinical indications, procedure technique and clinical outcome in all patients referred to Interventional Radiology for the percutaneous management of renal calyceal diverticula. Materials and methods: A retrospective review of all patients treated with percutaneous balloon dilatation of calyceal diverticular infundibula in the Interventional Radiology Department over a 10-year period was performed. Data collected included clinical details, laboratory indices, IVP and CT findings, procedure details and clinical outcome. Results: A total of three patients were treated over the course of the study period. All patients were young females who presented as a result of recurrent urinary tract infections. The calyceal diverticula were diagnosed incidentally on routine IVPs, with CT eloquently confirming the plain film findings in two cases. Technical procedure success was achieved in all cases, avoiding surgical intervention. Clinical follow-up revealed no further UTIs following dilatation. Conclusion: Fluoroscopic guided percutaneous balloon dilatation of renal calyceal diverticular infundibula following direct diverticular puncture is a safe and well tolerated method to reduce UTI and potentially avoid future stone formation.

  9. Phytochemical analysis, antioxidant and anti-inflammatory activity of calyces from Physalis peruviana.

    Science.gov (United States)

    Toro, Reina M; Aragón, Diana M; Ospina, Luis F; Ramos, Freddy A; Castellanos, Leonardo

    2014-11-01

    Physalis peruviana calyces are used extensively in folk medicine. The crude ethanolic extract and some fractions of calyces were evaluated in order to explore antioxidant and anti-inflammatory activities. The anti-inflammatory activity was evaluated by the TPA-induced ear edema model. The antioxidant in vitro activity was measured by means of the superoxide and nitric oxide scavenging activity of the extracts and fractions. The butanolic fraction was found to be promising due to its anti-inflammatory and antioxidant activities. Therefore, a bio-assay guided approach was employed to isolate and identify rutin (1) and nicotoflorin (2) from their NMR spectroscopic and MS data. The identification of rutin in calyces of P. peruviana supports the possible use of this waste material for phytotherapeutic, nutraceutical and cosmetic preparations.

  10. Clinical Manifestation of a Calyceal Diverticular Abscess in a Pregnant Woman

    Directory of Open Access Journals (Sweden)

    Masaki Sekiguchi

    2014-01-01

    Full Text Available Calyceal diverticula are congenital, nonsecretory abnormalities in which the transitional cell-lined cavity communicates with the renal collecting system. Here we present the case of a calyceal diverticular abscess during pregnancy. A 40-year-old primiparous woman developed the abscess at 23 weeks of gestation, with right flank pain and a 37.8°C fever. A transabdominal ultrasound revealed a 12×10 cm cystic mass in the right kidney. She was initially diagnosed with a simple renal cyst infection, and intravenous antibiotics were initiated. Percutaneous drainage was started at 26 weeks of gestation. When urine excretion from the cyst was confirmed by dye test using indigotindisulfonate sodium, the patient was diagnosed with a calyceal diverticular abscess. She gave birth to a 2,870 g healthy male at 38 weeks of gestation. Percutaneous drainage with low-dose antimicrobial therapy could thus allow for the continued pregnancy of women with a calyceal diverticular abscess until full term.

  11. Effect of Hibiscus sabdariffa L. Dried Calyx Ethanol Extract on Fat Absorption-Excretion, and Body Weight Implication in Rats

    Directory of Open Access Journals (Sweden)

    O. Carvajal-Zarrabal

    2009-01-01

    , and . Only did not show significant increases in weight, food consumption and efficiency compared to . The opposite occurred in group which showed a significant decrease for these three parameters. The responses were similar to , with the exception of food consumption. In both and groups, no body weight loss was observed; however, only in the latter group was there a significantly greater amount of fatty acids found in feces. A collateral effect emerging from the study is that components of Hs extract at the intermediate and greater concentrations used in this experiment could be considered possible antiobesity agents.

  12. Resistance to antimicrobials and acid and bile tolerance of Bacillus spp isolated from Bikalga, fermented seeds of Hibiscus sabdariffa

    DEFF Research Database (Denmark)

    Compaore, Clarisse S.; Jensen, Lars Bogø; Diawara, Brehima

    2013-01-01

    In the aim of selecting starter cultures, thirteen species of Bacillus spp. including six Bacillus subtilis ssp. subtilis, four Bacillus licheniformis and three Bacillus amyloliquefaciens ssp. plantarum isolated from traditional Bikalga were investigated. The study included, for all isolates, gen...

  13. Control de plagas de la jamaica (Hibiscus sabdariffa L. con Gliricidia sepium (Jacq. en Chiautla de Tapia, Puebla

    Directory of Open Access Journals (Sweden)

    A. Aragón-García

    2008-01-01

    Full Text Available Durante el ciclo agrícola comprendido entre los meses de julio a diciembre de 2005, se generó en el municipio de Chiautla de Tapia, una metodología para el control de plagas insectiles que afectan el follaje del cultivo de jamaica. En el desarrollo de este trabajo se utilizó una parcela ubicada en el mismo municipio, en la que se sembrójamaica criolla -de acuerdo a la tecnología agrícola de la zona- y se controlaron las plagas, realizando cuatro aplicaciones de extracto acuoso de Gliricidia sepium (Jacq., alternándolas con cuatro aplicaciones de jabón neutro. Para verificar la efectividad del tratamiento, los parámetros evaluados fueron: porcentaje de infestación, daños causados por insectos y producción. Los resultados obtenidos indican que la parcela a la cual se consideró como testigo, presentó un porcentaje de infestación del 79%; mientras que la parcela que se le aplicó el tratamiento presentó un 21% de infestación. En cuanto a los daños, en la parcela testigo se presentó en promedio 30.08% de daño por planta y la parcela donde se realizó el control sólo 14.89%. La producción en la parcela tratada fue de 793 kg/ha, presentándose un incremento en la producción con respecto al testigo, del 116%. En los tres parámetros evaluados se observó diferencia significativa ( = 0.05 entre tratamientos. Con la aplicación del tratamiento, se evitan los daños a las plantas, observándose un incremento en la producción.

  14. Hibiscus plant named `Sahara Sunset` U.S. Plant Patent 21,765

    Science.gov (United States)

    'Sahara Sunset' is a new and distinct cultivar of Hibiscus, botanically known as Hibiscus acetosella. The new Hibiscus was originated in Poplarville, Miss. and is a product of a mutation induction program. The parent of the present new cultivar is an unknown Hibiscus acetosella Wels. Ex Hiern seedli...

  15. 'USS Alabama', 'USS Mississippi', 'USS Missouri', 'USS Tennessee' and 'USS Texas' Chinese hibiscus (Hibiscus rosa-sinenis L.)

    Science.gov (United States)

    Five new Chinese hibiscus cultivars are products from a planned hibiscus breeding program conducted by the inventors at Poplarville, MS. The goal of the research is to produce new clones with a combination of desirable horticultural traits. Desirable floral traits include early bloom, uniform flower...

  16. Visual inputs to the mushroom body calyces of the whirligig beetle Dineutus sublineatus: modality switching in an insect.

    Science.gov (United States)

    Lin, Chan; Strausfeld, Nicholas J

    2012-08-15

    The mushroom bodies are prominent lobed centers in the forebrain, or protocerebrum, of most insects. Previous studies on mushroom bodies have focused on higher olfactory processing, including olfactory-based learning and memory. Anatomical studies provide strong support that in terrestrial insects with mushroom bodies, the primary input region, or calyces, are predominantly supplied by olfactory projection neurons from the antennal lobe glomeruli. In aquatic species that generally lack antennal lobes, the calyces are vestigial or absent. Here we report an exception to this in the whirligig beetle Dineutus sublineatus (Coleoptera: Gyrinidae). This aquatic species lives on water and is equipped with two separate pairs of compound eyes, one pair viewing above and one viewing below the water surface. As in other aquatic insects, the whirligig beetle lacks antennal lobes, but unlike other aquatic insects its mushroom bodies possess robust calyces. Golgi impregnations and fluorescent tracer injections revealed that the calyces are exclusively supplied by visual neurons from the medulla of the dorsal eye optic lobes. No other sensory inputs reach the calyces, thereby showing a complete switch of calyx modality from olfaction to vision. Potential functions of the mushroom bodies of D. sublineatus are discussed in the context of the behavioral ecology of whirligig beetles.

  17. Changes in the quality of zobo beverages produced from Hibiscus ...

    African Journals Online (AJOL)

    SERVER

    2008-04-17

    Apr 17, 2008 ... Quality changes in zobo beverage produced from Hibiscus sabdarifa during storage and the ... Zobo drink, a non alcoholic local beverage is produced ..... treatment on the shelf life of fermented African oil bean seed (Ugbu),.

  18. Free radical scavenging activities of pigment extract from Hibiscus ...

    African Journals Online (AJOL)

    AJL

    2012-01-05

    Jan 5, 2012 ... 3College of Pharmacy, Xinxiang Medical University, Xinxiang 453003, China. Accepted ... commerce for economic and environmental reasons. Hibiscus ..... sinensis leaves and tea from a lowland plantation in Malaysia. Food.

  19. The Influences of Lime Powder (Citrus aurantifolia, S. on Hibiscus Leaf Tea Quality (Hibiscus rosasinensis, L.

    Directory of Open Access Journals (Sweden)

    Sahadi Didi Ismanto

    2015-01-01

    Full Text Available The first aim of the study is to look the influence of different lime powder concentration level addition tohibiscus leaf dip drink’s quality. Then, the Second influence of different lime powder for tea hibiscus leaf which accepted as best sensory analysis. The study was conducted from August to September 2014 in the Agricultural Technology Laboratory and Tablet preparation laboratory Pharmacy, University of Andalas, Padang Indonesia. This study is used complete randomized design (CRD consisting of 4 treatments and 3 replications. The data were analyzed by using ANOVA statistically and Duncan’s New Multiple Range (DNMRT at5% significance level. The Treatment in this study is the addition of lime powder by 55%, 65%, 75%, and85%. Observations of hibiscus leaf powder and lime powder such us water levels, ash levels, vitamin C and antioxidants, while product tea hibiscus leaf such water levels, ash levels, vitamin C, antioxidants, poly phenols, sensory analysis, and total plate count of it. The result of the study is showed that the addition of lime powder influence on water levels, ash levels, vitamin C, and antioxidants. Based sensory analysis and chemical analysis, the best beverage products hibiscus leaves are dipped in treatment A (addition of lime powder 55% with a 45% level of liking the taste, flavor and color of 75% and 55% chemical step, vitamin C 0.15%, 1.58% total poly phenols, antioxidant activity of 35.51%, total plate count was 2.83 × 103 cfu/ml, 7.76% water levels and ash levels of 9.87%.

  20. Biological and molecular detection of Hibiscus chlorotic ringspot virus infecting Hibiscus rosa-sinensis in Iran

    Directory of Open Access Journals (Sweden)

    Reza POURRAHIM

    2014-01-01

    Full Text Available During 2011, ten leaf samples of Hibiscus rosa-sinensis with virus-like symptoms including diffuse mottling, numerous chlorotic spots and chlorotic ring spots, were collected from Guilan province, North Iran. The results of mechanical inoculation of sap from these leaves onto different indicator hosts showed local lesions (chlorotic or necrotic on Gompherena globosa, Chenopodium amaranticolor, C. quinoa and Gossypium hirsutum, while Hibiscus cannabinus (kenaf showed systemic chlorotic ring spot symptoms. Purified preparations of the isolated virus contained isometric particles approximately 28–30 nm in diameter. A specific band of about 1.3 kb was amplified from all symptomatic leaves using Hibiscus chlorotic ringspot virus (HCRSV specific primers. Comparative sequence analysis revealed that the IRN.HCRSV (JX865593 isolate shared the greatest nucleotide sequence identity (96.5% with NC-003608 (Singapore and the least nt sequence identity (91.5% with DQ392986 (Taiwan isolates. The phylogenetic tree showed at least two subgroups for HCRSV isolates in which the Iranian isolate was grouped with Singapore HCSRV (NC-003608. This is the first report of HCRSV in H. rosa-sinensis in Iran.

  1. Effect of Roselle calyces extract on the chemical and sensory properties of functional cupcakes

    Directory of Open Access Journals (Sweden)

    A.R. Abdel-Moemin

    2016-12-01

    Full Text Available Roselle calyces (RC are a major crop for export and used to make a common drink in Egypt. Dried RC are commercially available and appreciated to obtain concentrated extracts which might be used in the food and pharmaceutical industries for color and heath benefits. The objective of this research was to determine the chemical and the sensory properties of cupcakes formulated with Roselle calyces extract (RCE. Proximate analysis, anthocyanins, ascorbic acid, titrable acidity, % retaining of anthocyanins, color and sensory evaluations were done. RC cupcakes had high sensory scores (P < 0.05 compared to control cupcakes. The parameter a* was significantly red in the RC cupcakes compared to control cupcakes along with 77% retaining of anthocyanins. The consumption of 100 g of the RC cupcakes would provide 465 mg/100 g dry matter anthocyanin that is more than 2 folds of the minimum average of the daily intake of anthocyanins for Americans, along ∼1/3 of the daily dietary fiber intake to achieve fiber adequacy according to the Scientific Advisory Committee on Nutrition. RC cupcake can be a functional food and would have a “clean” label with cost effective advantage.

  2. Sucrose esters from Physalis peruviana calyces with anti-inflammatory activity.

    Science.gov (United States)

    Franco, Luis A; Ocampo, Yanet C; Gómez, Harold A; De la Puerta, Rocío; Espartero, José L; Ospina, Luis F

    2014-11-01

    Physalis peruviana is a native plant from the South American Andes and is widely used in traditional Colombian medicine of as an anti-inflammatory medicinal plant, specifically the leaves, calyces, and small stems in poultice form. Previous studies performed by our group on P. peruviana calyces showed potent anti-inflammatory activity in an enriched fraction obtained from an ether total extract. The objective of the present study was to obtain and elucidate the active compounds from this fraction and evaluate their anti-inflammatory activity in vivo and in vitro. The enriched fraction of P. peruviana was purified by several chromatographic methods to obtain an inseparable mixture of two new sucrose esters named peruviose A (1) and peruviose B (2). Structures of the new compounds were elucidated using spectroscopic methods and chemical transformations. The anti-inflammatory activity of the peruvioses mixture was evaluated using λ-carrageenan-induced paw edema in rats and lipopolysaccharide-activated peritoneal macrophages. Results showed that the peruvioses did not produce side effects on the liver and kidneys and significantly attenuated the inflammation induced by λ-carrageenan in a dosage-dependent manner, probably due to an inhibition of nitric oxide and prostaglandin E2, which was demonstrated in vitro. To our knowledge, this is the first report of the presence of sucrose esters in P. peruviana that showed a potent anti-inflammatory effect. These results suggest the potential of sucrose esters from the Physalis genus as a novel natural alternative to treat inflammatory diseases.

  3. Comparative analysis of nutritional composition of Amaranthus ...

    African Journals Online (AJOL)

    Comparative analysis of nutritional composition of Amaranthus caudatus ... and Hibiscus sabdariffa (Roselle), using standard analytical methods The result ... content (74.8%) and lipid content (5.5%), while Hibiscus sabdariffa (Roselle) contain ...

  4. MARKS OF ETHNICITY IN PURPLE HIBISCUS TRANSLATION

    Directory of Open Access Journals (Sweden)

    Fernanda de Oliveira Müller

    2016-10-01

    Full Text Available This study gives an analysis of the English – Brazilian Portuguese translation of Chimamanda Ngozi Adichie’s novel, Purple Hibiscus, made by Julia Romeu. It is an attempt to analyze how traces of ethnic identities marked in the source text are reproduced in the Brazilian version Hibisco Roxo, published in 2011. Initially, is a brief biography of the writer is presented together with her history towards the construction of a new paradigm for the literature about Africa and Nigeria. Adichie challenges Western stereotypes about that continent, which tend to report poverty, war and disease scenarios. Secondly, a summary of the story was made and the main characters were described. Thirdly, a collection of recorded words and phrases in the Igbo language was compiled from the original text and an analysis of the translation of those terms into Brazilian Portuguese was performed. Afterwards, the concept of ethnicity described by the sociologist Anthony Giddens was presented. Based on that concept, it was concluded that the terms previously selected could be considered as marks of ethnicity, reflecting the presence of the Igbo ethnic group in the British colonial culture. Finally, taking Antoine Berman’s proposition for an ethical translation, which embraces the foreign and rejects ethnocentrism, the conclusion to be drawn is that the translator’s option to keep Igbo terms in her work respected the author’s manifest intention of, through her work, showing the readers from other countries a bit of Nigeria’s culture and history.

  5. An overview of the HIBISCUS campaign

    Directory of Open Access Journals (Sweden)

    J.-P. Pommereau

    2007-02-01

    Full Text Available HIBISCUS was a field campaign for investigating the impact of deep convection on the Tropical Tropopause Layer (TTL and the Lower Stratosphere, which took place during the Southern Hemisphere summer in February–March 2004 in the State of São Paulo, Brazil. Its objective was to provide a set of new observational data on meteorology, tracers of horizontal and vertical transport, water vapour, clouds, and chemistry in the tropical UT/LS from balloon observations at local scale over a land convective area, as well as at global scale using circumnavigating long-duration balloons. Overall, the composition of the TTL, the region between 14 and 19 km of intermediate lapse rate between the almost adiabatic upper troposphere and the stable stratosphere, appears highly variable. Tracers and ozone measurements performed at both the local and the global scale indicate a strong quasi-horizontal isentropic exchange with the lowermost mid-latitude stratosphere suggesting that the barrier associated to the tropical jet is highly permeable at these levels in summer. But the project also provides clear indications of strong episodic updraught of cold air, short-lived tracers, low ozone, humidity and ice particles across the lapse rate tropopause at about 15 km, up to 18 or 19 km at 420–440 K potential levels in the lower stratosphere, suggesting that, in contrast to oceanic convection penetrating little the stratosphere, fast daytime developing land convective systems could be a major mechanism in the troposphere-stratosphere exchange at the global scale.

    The present overview is meant to provide the background of the project, as well as overall information on the instrumental tools available, on the way they have been used within the highly convective context of the South Atlantic Convergence Zone, and a brief summary of the results, which will be detailed in several other papers of this special issue.

  6. Maturation of auditory brainstem projections and calyces in the congenitally deaf (dn/dn) mouse.

    Science.gov (United States)

    Youssoufian, Monique; Couchman, Kiri; Shivdasani, Mohit N; Paolini, Antonio G; Walmsley, Bruce

    2008-01-20

    The deaf dn/dn mouse is a valuable model of human congenital deafness. In this study we used the lipophylic dye DiA to trace auditory nerve and cochlear nucleus projections in the dn/dn mouse. In both normal and deaf mice, the ipsilateral projections from the anteroventral cochlear nucleus (AVCN) to the lateral superior olive (LSO), and the contralateral projections from the AVCN to the medial nucleus of the trapezoid body (MNTB) were intact. With age, there was a noted increase in the fenestration of the endbulb and calyx of Held, and this morphological maturation was also observed in the deaf mice, although there was a significant difference in total endbulb volume at P20 between normal and deaf mice. However, total calyceal volume was not significantly different between normal and deaf mice. There was electrophysiological evidence of in vivo spontaneous ventral cochlear nucleus activity in normal and deaf animals, indicating that this activity may be responsible for the appropriate connectivity in the deaf mice. Our results indicate that congenital deafness caused by the dn/dn mutation does not result in aberrant projections between the AVCN and the ipsilateral MNTB and contralateral LSO but can cause abnormalities in endbulb size.

  7. WITHDRAWN: Corrigendum to "Morphological characteristics and pathogenicity of fungi associated with Roselle (Hibiscus Sabdariffa) diseases in Penang, Malaysia" [Microb Pathog (2011) 325-337].

    Science.gov (United States)

    Eslaminejad, Touba; Zakaria, Maziah

    2012-06-01

    The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.micpath.2012.05.010;. The duplicate article has therefore been withdrawn.

  8. Effect of stevia and citric acid on the stability of phenolic compounds and in vitro antioxidant and antidiabetic capacity of a roselle (Hibiscus sabdariffa L.) beverage.

    Science.gov (United States)

    Pérez-Ramírez, Iza F; Castaño-Tostado, Eduardo; Ramírez-de León, José A; Rocha-Guzmán, Nuria E; Reynoso-Camacho, Rosalía

    2015-04-01

    Plant infusions are consumed due to their beneficial effects on health, which is attributed to their bioactive compounds content. However, these compounds are susceptible to degradation during processing and storage. The objective of this research was to evaluate the effect of stevia and citric acid on the stability of phenolic compounds, antioxidant capacity and carbohydrate-hydrolysing enzyme inhibitory activity of roselle beverages during storage. The optimum extraction conditions of roselle polyphenolic compounds was of 95 °C/60 min, which was obtained by a second order experimental design. The incorporation of stevia increased the stability of colour and some polyphenols, such as quercetin, gallic acid and rosmarinic acid, during storage. In addition, stevia decreased the loss of ABTS, DPPH scavenging activity and α-amylase inhibitory capacity, whereas the incorporation of citric acid showed no effect. These results may contribute to the improvement of technological processes for the elaboration of hypocaloric and functional beverages.

  9. Biodiversity of aerobic endospore-forming bacterial species occurring in Yanyanku and Ikpiru, fermented seeds of Hibiscus sabdariffa used to produce food condiments in Benin

    DEFF Research Database (Denmark)

    Agbobatinkpo, Pélagie B.; Thorsen, Line; Nielsen, Dennis Sandris

    2013-01-01

    toxin encoding genes cytK-1, cytK-2, hblA, hblC, and hblD were present in 0%, 91% 15%, 34% and 35% of B. cereus isolates, respectively. 9% of them harbored the emetic toxin genetic determinant, cesB. This study is the first to identify the AEFB of Yanyanku and Ikpiru to species level and perform...... a safety evaluation based on toxin gene detections. We further suggest, that the gyrA gene can be used for differentiating the closely related species Bacillus pumilus and B. safensis....

  10. Comparison of flexible ureterorenoscopy and mini-percutaneous nephrolithotomy in treatment of lower calyceal stones smaller than 2 cm.

    Science.gov (United States)

    Akbulut, Fatih; Kucuktopcu, Onur; Kandemir, Emre; Sonmezay, Erkan; Simsek, Abdulmuttalip; Ozgor, Faruk; Binbay, Murat; Muslumanoglu, Ahmet Yaser; Gurbuz, Gokhan

    2016-01-01

    To compare the outcomes of flexible ureterorenoscopy (F-URS) and mini-percutaneous nephrolithotomy (mini-PNL) in the treatment of lower calyceal stones smaller than 2 cm. Patients who underwent F-URS and mini-PNL for the treatment of lower calyceal stones smaller than 2 cm between March 2009 and December 2014 were retrospectively evaluated. Ninety-four patients were divided into two groups by treatment modality: F-URS (Group 1: 63 patients) and mini-PNL (Group 2: 31 patients). All patients were preoperatively diagnosed with intravenous pyelography or computed tomography. Success rates for F-URS and mini-PNL at postoperative first month were 85.7% and 90.3%, respectively. Operation time, fluoroscopy time, and hospitalization time for F-URS and mini-PNL patients were 44.40 min, 2.9 min, 22.4 h, and 91.9 min, 6.4 min, and 63.8 h, respectively. All three parameters were significantly shorter among the F-URS group (p PNL group (0.39 mg/dL vs. 1.15 mg/dL, p = 0.001). A comparison of complications according to the Clavien classification demonstrated significant differences between the groups (p = 0.001). More patients in the F-URS groups require antibiotics due to urinary tract infection, and more patients in the mini-PNL group required ureteral double J catheter insertion under general anesthesia. Although both F-URS and mini-PNL have similar success rates for the treatment of lower calyceal stones, F-URS appears to be more favorable due to shorter fluoroscopy and hospitalization times; and lower hemoglobin drops. Multicenter and studies using higher patient volumes are needed to confirm these findings.

  11. Comparative Analysis of the Properties of Acid-Base Indicator of Rose (Rosa setigera), Allamanda (Allamanda cathartica), and Hibiscus (Hibiscus rosa-sinensis) Flowers

    OpenAIRE

    Okoduwa, Stanley I. R.; Mbora, Lovina O.; Adu, Matthew E.; Ameh A. Adeyi

    2015-01-01

    The need to develop effective alternative for synthetic indicators is the demand of present-day chemistry. The acid-base indicator properties of Rose (Rosa setigera), Allamanda (Allamanda cathartica), and Hibiscus (Hibiscus rosa-sinensis) flowers were examined. Colour pigments were extracted from the flowers via cold and solvent extraction using soxhlet extractor. The pH value of the extracts with wavelengths of absorption was determined using ultraviolet spectrophotometer. From the results o...

  12. Carotenoids and Carotenoid Esters of Red and Yellow Physalis (Physalis alkekengi L. and P. pubescens L.) Fruits and Calyces.

    Science.gov (United States)

    Wen, Xin; Hempel, Judith; Schweiggert, Ralf M; Ni, Yuanying; Carle, Reinhold

    2017-08-02

    Carotenoid profiles of fruits and calyces of red (Physalis alkekengi L.) and yellow (P. pubescens L.) Physalis were characterized by HPLC-DAD-APCI-MS(n). Altogether 69 carotenoids were detected in red Physalis, thereof, 45 were identified. In yellow Physalis, 40 carotenoids were detected and 33 were identified. Zeaxanthin esters with various fatty acids were found to be the most abundant carotenoids in red Physalis, accounting for 51-63% of total carotenoids, followed by β-cryptoxanthin esters (16-24%). In yellow Physalis, mainly free carotenoids such as lutein and β-carotene were found. Total carotenoid contents ranged between 19.8 and 21.6 mg/100 g fresh red Physalis fruits and 1.28-1.38 mg/100 g fresh yellow Physalis fruits, demonstrating that Physalis fruits are rich sources of dietary carotenoids. Yellow Physalis calyces contained only 153-306 μg carotenoids/g dry weight, while those of red Physalis contained substantially higher amounts (14.6-17.6 mg/g dry weight), thus possibly exhibiting great potential as a natural source for commercial zeaxanthin extraction.

  13. Silver Nano Scaffold Formation by Flowers of Hibiscus Rosa Sinensis

    Directory of Open Access Journals (Sweden)

    S.Shabana

    2013-06-01

    Full Text Available Hibiscusrosa sinensis grows for their showy flowers and are used as landscape shrubs. Hibiscusrosa sinensis also has too many medicinal properties. Flowers are rich in polyphenols, flavonoids and anthocyanins. Inourstudy the flower extracts of Hibiscus rosa sinensis has been used for the synthesis of silver nano scaffold formation and its reduction by the flowers. The synthesis of silver nanoparticles occurred under the exposure of the flower extract to 1mM (AgNO3, Silver nitrate aqueous solution. During this process the complete reduction of nanoparticles was observed nearly 48hours of reaction at 30°C under vigorous shaking conditions. The colour change is noted in the reaction mixture and observed during the process of incubation period, it may be due to the formation of the silver nanoparticles which are able to produce the colour in the reaction mixture may be due to their specific properties of surface Plasmon resonance. The colour change was made confirmed by visualization and the characterization by FT-IR, UV-Visible Spectroscopy. Thus we conclude that the reduction process and capping may be having occurred due to the presence of many flavonoids, terpenoids, anthocyanins, some aminoacids and proteins. In conclusion, Hibiscus rosa sinensis flower extract appears capable of forming silver nanoparticles. It can be concluded that, Hibiscus rosa sinensis flower extract was able to form the silver nano scaffold and may be probably due to its antioxidants potential, some flavonoids, terpenes etc. This study is a preliminary effort and requires further investigation at different levels.

  14. Deciduous shrubs for ozone bioindication: Hibiscus syriacus as an example

    Energy Technology Data Exchange (ETDEWEB)

    Paoletti, Elena [Institut Plant Protection (IPP), National Council Research (CNR), Via Madonna del Piano 10, 50019 Sesto Fiorentino, Florence (Italy)], E-mail: e.paoletti@ipp.cnr.it; Ferrara, Anna Maria [Istituto per le Piante da Legno e l' Ambiente (IPLA), Corso Casale 476, 10132 Turin (Italy); Calatayud, Vicent; Cervero, Julia [Fundacion C.E.A.M., Charles R. Darwin 14, Parc Tecnologic, 46980 Paterna, Valencia (Spain); Giannetti, Fabio [Istituto per le Piante da Legno e l' Ambiente (IPLA), Corso Casale 476, 10132 Turin (Italy); Sanz, Maria Jose [Fundacion C.E.A.M., Charles R. Darwin 14, Parc Tecnologic, 46980 Paterna, Valencia (Spain); Manning, William J. [Department of Plant, Soil and Insect Sciences, University of Massachusetts, Amherst, MA 01003-9320 (United States)

    2009-03-15

    Ozone-like visible injury was detected on Hibiscus syriacus plants used as ornamental hedges. Weekly spray of the antiozonant ethylenediurea (EDU, 300 ppm) confirmed that the injury was induced by ambient ozone. EDU induced a 75% reduction in visible injury. Injury was more severe on the western than on the eastern exposure of the hedge. This factor of variability should be considered in ozone biomonitoring programmes. Seeds were collected and seedlings were artificially exposed to ozone in filtered vs. not-filtered (+30 ppb) Open-Top Chambers. The level of exposure inducing visible injury in the OTC seedlings was lower than that in the ambient-grown hedge. The occurrence of visible injury in the OTC confirmed that the ozone sensitivity was heritable and suggested that symptomatic plants of this deciduous shrub population can be successfully used as ozone bioindicators. EDU is recommended as a simple tool for diagnosing ambient ozone visible injury on field vegetation. - An Italian population of the deciduous shrub Hibiscus syriacus, a common ornamental species in temperate zones, is recommended as ozone bioindicator.

  15. Kenaf (Hibiscus cannabinus) and sunn hemp (Crotalaria juncea): Monoculture and polyculture production

    Science.gov (United States)

    Kenaf (Hibiscus cannabinus L.) and sunn hemp (Crotalaria juncea L.) are fast growing summer annual crops with numerous commercial applications (fibers, biofuels, bioremediation, paper pulp, building materials, cover crops, and livestock forages). Field research was conducted in southeast Oklahoma (...

  16. Full-genome sequence of hibiscus chlorotic ringspot virus from Israel.

    Science.gov (United States)

    Luria, Neta; Reingold, Victoria; Lachman, Oded; Dombrovsky, Aviv

    2013-12-12

    Hibiscus rosa-sinensis is one of the most prevalent ornamental plants grown in private and public gardens. Hibiscus chlorotic ringspot virus (HCRSV) is a member of the Carmovirus genus, with a positive single-strand RNA that putatively encodes seven proteins. The complete genome of the first Israeli isolate of HCRSV, HCRSV-IL, comprises 3,908 nucleotides and shows 93% nucleotide sequence identity to the Singapore isolate and 87% identity to the Taiwanese isolate.

  17. Spatial and temporal transcriptome changes occurring during flower opening and senescence of the ephemeral hibiscus flower, Hibiscus rosa-sinensis

    Science.gov (United States)

    Trivellini, Alice; Cocetta, Giacomo; Hunter, Donald A.; Vernieri, Paolo; Ferrante, Antonio

    2016-01-01

    Flowers are complex systems whose vegetative and sexual structures initiate and die in a synchronous manner. The rapidity of this process varies widely in flowers, with some lasting for months while others such as Hibiscus rosa-sinensis survive for only a day. The genetic regulation underlying these differences is unclear. To identify key genes and pathways that coordinate floral organ senescence of ephemeral flowers, we identified transcripts in H. rosa-sinensis floral organs by 454 sequencing. During development, 2053 transcripts increased and 2135 decreased significantly in abundance. The senescence of the flower was associated with increased abundance of many hydrolytic genes, including aspartic and cysteine proteases, vacuolar processing enzymes, and nucleases. Pathway analysis suggested that transcripts altering significantly in abundance were enriched in functions related to cell wall-, aquaporin-, light/circadian clock-, autophagy-, and calcium-related genes. Finding enrichment in light/circadian clock-related genes fits well with the observation that hibiscus floral development is highly synchronized with light and the hypothesis that ageing/senescence of the flower is orchestrated by a molecular clock. Further study of these genes will provide novel insight into how the molecular clock is able to regulate the timing of programmed cell death in tissues. PMID:27591432

  18. Decrease of Plasma Glucose by Hibiscus taiwanensis in Type-1-Like Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Lin-Yu Wang

    2013-01-01

    Full Text Available Hibiscus taiwanensis (Malvaceae is widely used as an alternative herb to treat disorders in Taiwan. In the present study, it is used to screen the effect on diabetic hyperglycemia in streptozotocin-induced diabetic rats (STZ-diabetic rats. The extract of Hibiscus taiwanensis showed a significant plasma glucose-lowering action in STZ-diabetic rats. Stems of Hibiscus taiwanensis are more effective than other parts to decrease the plasma glucose in a dose-dependent manner. Oral administration of Hibiscus taiwanensis three times daily for 3 days into STZ-diabetic rats increased the sensitivity to exogenous insulin showing an increase in insulin sensitivity. Moreover, similar repeated administration of Hibiscus taiwanensis for 3 days in STZ-diabetic rats produced a marked reduction of phosphoenolpyruvate carboxykinase (PEPCK expression in liver and an increased expression of glucose transporter subtype 4 (GLUT 4 in skeletal muscle. Taken together, our results suggest that Hibiscus taiwanensis has the ability to lower plasma glucose through an increase in glucose utilization via elevation of skeletal GLUT 4 and decrease of hepatic PEPCK in STZ-diabetic rats.

  19. Deciduous shrubs for ozone bioindication: Hibiscus syriacus as an example.

    Science.gov (United States)

    Paoletti, Elena; Ferrara, Anna Maria; Calatayud, Vicent; Cerveró, Júlia; Giannetti, Fabio; Sanz, María José; Manning, William J

    2009-03-01

    Ozone-like visible injury was detected on Hibiscus syriacus plants used as ornamental hedges. Weekly spray of the antiozonant ethylenediurea (EDU, 300ppm) confirmed that the injury was induced by ambient ozone. EDU induced a 75% reduction in visible injury. Injury was more severe on the western than on the eastern exposure of the hedge. This factor of variability should be considered in ozone biomonitoring programmes. Seeds were collected and seedlings were artificially exposed to ozone in filtered vs. not-filtered (+30ppb) Open-Top Chambers. The level of exposure inducing visible injury in the OTC seedlings was lower than that in the ambient-grown hedge. The occurrence of visible injury in the OTC confirmed that the ozone sensitivity was heritable and suggested that symptomatic plants of this deciduous shrub population can be successfully used as ozone bioindicators. EDU is recommended as a simple tool for diagnosing ambient ozone visible injury on field vegetation.

  20. Resin polymer and corrosion casting of the porcine pelvi-calyceal system: a useful model for investigating new imaging and endoscopic techniques of the upper urinary tract.

    Science.gov (United States)

    John, Babbin; Ghani, Khurshid R; Patel, Uday; Anson, Ken

    2008-02-01

    We describe the use of polyester resin casting of the pelvi-calyceal (PC) system as a method of evaluating the accuracy of new three-dimensional imaging technology such as ultrasound and computerised tomography. Thirty-eight kidneys from large white pigs were used for the study. We describe the process of preparation of the kidney and polyester resin for injection into the PC systems. The setting process of the resin is an exothermic reaction with an associated change in consistency. The PC systems of the kidneys were injected with resin in a controlled manner and casts obtained by maceration of the kidneys. Some of these kidneys had been distended previously with 11% glycerol and three-dimensional ultrasound reconstructions of their PC systems were compared to resin casts to assess accuracy of the reconstructions. Thirty-eight casts were created out of which 13 were poor. The quality of the casts improved with practice and pelvi-calyceal morphology could be faithfully recreated. Controlled perfusion and watching for signs such as a "turgid feel" of the kidney help avoid pelvi-calyceal disruption. Anatomically accurate casts of the kidney PC system can be created using polyester resin with the technique described. These casts can be a useful research and training tool with urological and radiological applications.

  1. Notice to Nurserymen of the Naming and Release for Propagation of Sahara Sunset, A New African Hibiscus Cultivar

    Science.gov (United States)

    Hibiscus asetosella 'Sahara Sunset' is an African hibiscus released by the United States Department of Agriculture Agricultural Research Service that was Developed by Dr. Cecil Pounders at the Thad Cochran Southern Horticultural Laboratory in Poplarville, MS. ‘Sahara Sunset’ is a tropical shrub (USD...

  2. Spastic paraparesis as a complication of percutaneous nephrolithothripsy (PNL) on a calyceal calculus of the left kidney.

    Science.gov (United States)

    Pellegrinelli, Moira; Castiglioni, Claudia; Morini, Osvaldo; Franzini, Aldo

    2007-12-01

    A patient developed spastic paraparesis after surgery with ultrasound lithothripsy and litholapaxy of fragments of a renal calyceal calculus in middle-upper diverticulum. It was first assumed that the event could be due to transient spinal ischemia, caused by vasospasm of Adamkiewicz artery, secondary to blood engorgement of the area around the vessel. In order to clarify possible implications of medical liability, the Authors took into account the etio-pathogenetic mechanisms of the complication and analyzed the medico-legal aspects, with particular reference to the indication for surgery, which was not absolute in the case under scrutiny. In connection with the latter aspect, the Authors considered the conclusions of a recent sentence of the Court of Milan, whereby, despite the negative opinion of the experts specifically appointed, a case of medical liability was identified as a consequence of algodystrophy resulting from a cardiosurgical intervention. According to the Court, it is for the medical staff to demonstrate that they did all they could to prevent the complication and that such complication did not arise from a mistake on their part.

  3. Breeding of Hibiscus rosa-sinensis for garden use in Denmark

    DEFF Research Database (Denmark)

    Kuligowska, Katarzyna; Simonsen, Maria Boye; Lütken, Henrik Vlk

    2013-01-01

    -sinensis for increased chilling tolerance by conventional breeding is unlikely because there is a lack of genetic information in relation to this trait within this species. In the Hibiscus genus, cold hardiness is available, but only in a few species. Attempts to create cold tolerant Hibiscus plants resembling H. rosa......Hibiscus rosa-sinensis is an ornamental shrub in tropical and subtropical regions. Limitation of its use as an outdoor ornamental plant in a temperate climate is due to chilling sensitivity. Cold hardiness is a highly complex trait, involving multiple genes. Improvement of H. rosa......-sinensis suitable for Nordic climate have not been successful so far. In ornamental breeding wide hybridization is a major source of genetic variation, and can also be a promising approach to introgress cold hardiness in H. rosa-sinensis. The objective of the present study is to evaluate the possibilities...

  4. Breeding of Hibiscus rosa-sinensis for garden use in Denmark

    DEFF Research Database (Denmark)

    Kuligowska, Katarzyna; Simonsen, Maria Boye; Lütken, Henrik Vlk

    2013-01-01

    Hibiscus rosa-sinensis is an ornamental shrub in tropical and subtropical regions. Limitation of its use as an outdoor ornamental plant in a temperate climate is due to chilling sensitivity. Cold hardiness is a highly complex trait, involving multiple genes. Improvement of H. rosa......-sinensis for increased chilling tolerance by conventional breeding is unlikely because there is a lack of genetic information in relation to this trait within this species. In the Hibiscus genus, cold hardiness is available, but only in a few species. Attempts to create cold tolerant Hibiscus plants resembling H. rosa......-sinensis suitable for Nordic climate have not been successful so far. In ornamental breeding wide hybridization is a major source of genetic variation, and can also be a promising approach to introgress cold hardiness in H. rosa-sinensis. The objective of the present study is to evaluate the possibilities...

  5. Fullerene-based symmetry in Hibiscus rosa-sinensis pollen.

    Directory of Open Access Journals (Sweden)

    Kleber Andrade

    Full Text Available The fullerene molecule belongs to the so-called super materials. The compound is interesting due to its spherical configuration where atoms occupy positions forming a mechanically stable structure. We first demonstrate that pollen of Hibiscus rosa-sinensis has a strong symmetry regarding the distribution of its spines over the spherical grain. These spines form spherical hexagons and pentagons. The distance between atoms in fullerene is explained applying principles of flat, spherical, and spatial geometry, based on Euclid's "Elements" book, as well as logic algorithms. Measurements of the pollen grain take into account that the true spine lengths, and consequently the real distances between them, are measured to the periphery of each grain. Algorithms are developed to recover the spatial effects lost in 2D photos. There is a clear correspondence between the position of atoms in the fullerene molecule and the position of spines in the pollen grain. In the fullerene the separation gives the idea of equal length bonds which implies perfectly distributed electron clouds while in the pollen grain we suggest that the spines being equally spaced carry an electrical charge originating in forces involved in the pollination process.

  6. Anti hypoxic and antioxidant activity of Hibiscus esculentus seeds

    Energy Technology Data Exchange (ETDEWEB)

    Ebrahimzadeh, M. A.; Nabavi, S. F.; Nabavi, S. M.; Eslami, B.

    2010-07-01

    The anti hypoxic and antioxidant activities of Hibiscus esculentus seeds were investigated employing eight in vitro assay systems. Anti hypoxic activity was investigated in two models, haemic and circulatory. The effects were pronounced in both models of hypoxia. The anti hypoxic effects were dose-dependent. The results indicated that the extracts have a protective effect against hypoxia induced lethality in mice. The extracts showed antioxidant activity in some models. IC{sub 5}0 for DPPH radical-scavenging activity was 234 {+-} 8.9 {mu}g ml{sup 1}. The extracts showed weak nitric oxide-scavenging activity between 0.1 and 1.6 mg ml{sup -}1. The extracts showed weak Fe{sup 2}+ chelating ability. IC{sub 5}0 were 150 {+-} 13 {mu}g ml{sup -}1. The extracts also exhibited low antioxidant activity in the linoleic acid model but were capable of scavenging hydrogen peroxide in a concentration dependent manner. The total amount of phenolic compounds in each extract was determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Pharmacological effects may be attributed, at least in part, to the presence of phenols and flavonoids in the extracts. (Author) 40 refs.

  7. Development of a computer assisted gantry system for gaining rapid and accurate calyceal access during percutaneous nephrolithotomy

    Directory of Open Access Journals (Sweden)

    A. D. Zarrabi

    2010-12-01

    Full Text Available PURPOSE: To design a simple, cost-effective system for gaining rapid and accurate calyceal access during percutaneous nephrolithotomy (PCNL. MATERIALS AND METHODS: The design consists of a low-cost, light-weight, portable mechanical gantry with a needle guiding device. Using C-arm fluoroscopy, two images of the contrast-filled renal collecting system are obtained: at 0-degrees (perpendicular to the kidney and 20-degrees. These images are relayed to a laptop computer containing the software and graphic user interface for selecting the targeted calyx. The software provides numerical settings for the 3 axes of the gantry, which are used to position the needle guiding device. The needle is advanced through the guide to the depth calculated by the software, thus puncturing the targeted calyx. Testing of the system was performed on 2 target types: 1 radiolucent plastic tubes the approximate size of a renal calyx (5 or 10 mm in diameter, 30 mm in length; and 2 foam-occluded, contrast-filled porcine kidneys. RESULTS: Tests using target type 1 with 10 mm diameter (n = 14 and 5 mm diameter (n = 7 tubes resulted in a 100% targeting success rate, with a mean procedure duration of 10 minutes. Tests using target type 2 (n = 2 were both successful, with accurate puncturing of the selected renal calyx, and a mean procedure duration of 15 minutes. CONCLUSIONS: The mechanical gantry system described in this paper is low-cost, portable, light-weight, and simple to set up and operate. C-arm fluoroscopy is limited to two images, thus reducing radiation exposure significantly. Testing of the system showed an extremely high degree of accuracy in gaining precise access to a targeted renal calyx.

  8. Comparative Analysis of the Properties of Acid-Base Indicator of Rose (Rosa setigera, Allamanda (Allamanda cathartica, and Hibiscus (Hibiscus rosa-sinensis Flowers

    Directory of Open Access Journals (Sweden)

    Stanley I. R. Okoduwa

    2015-01-01

    Full Text Available The need to develop effective alternative for synthetic indicators is the demand of present-day chemistry. The acid-base indicator properties of Rose (Rosa setigera, Allamanda (Allamanda cathartica, and Hibiscus (Hibiscus rosa-sinensis flowers were examined. Colour pigments were extracted from the flowers via cold and solvent extraction using soxhlet extractor. The pH value of the extracts with wavelengths of absorption was determined using ultraviolet spectrophotometer. From the results obtained, all the extracts exhibited sharp contrast between their colours in acid and base. Their pH was found to be 5.5 for cold extract of Rose and 5.6 for solvent extraction, 5.24 for cold extract of a Hibiscus and 6.52 for solvent extraction, 5.35 for cold extract of Allamanda, and 5.45 for solvent extraction. The maximum wavelengths of absorption obtained for all the extract fall within the visible region of electromagnetic spectrum. These values are almost similar to that obtained from synthetic indicators. It is on these bases that we concluded that natural indicators could be an excellent replacement for synthetic indicators since they are cheap, readily available, simple to extract, not toxic, user and environmentally friendly.

  9. Salicylic Acid Improved In Viro Meristem Regeneration and Salt Tolerance in Two Hibiscus Species

    Science.gov (United States)

    Salicylic acid (SA) has been reported to induce abiotic stress, including salt tolerance in plants. The objective of this study was to determine whether application of various exogenous SA concentrations to in vitro grown meristem shoots could induce salt tolerance in two Hibiscus species. The effec...

  10. Genes expressed in field-caught pink hibiscus mealybugs, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae)

    Science.gov (United States)

    We advanced the understanding of the biology of an invasive pest, the pink hibiscus mealybug, PHM, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae) by using a genomics approach to identify genes expressed within field collected PHM. The information produced provides valuable, new and unique info...

  11. Proteins expressed in the pink hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae)

    Science.gov (United States)

    We produced a dataset of 315 protein sequences which we isolated from the pink hibiscus mealybug, PHM, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae). The dataset was published under accession numbers: EF070444-EF070605 and EF092085-EF091933, in the National Center for Biotechnology Informatio...

  12. Validation of two pheromonal compounds for monitoring pink hibiscus mealybug in Mexico

    Science.gov (United States)

    A Pink Hibiscus Mealybug (Macconelicoccus hirsutus (Green)) was detected, in 2004, in Valle de Banderas, at municipalities Bahía de Banderas, Nayarit, and Puerto Vallarta, Jalisco, México, affecting fruit trees, native and ornamental plants. This pest insect is native to Asia and Australia, and has ...

  13. ‘Hapa White’, 'Hapa Pink', and 'Hapa Red' Interspecific Hybrid Hibiscus Cultivars

    Science.gov (United States)

    Hibiscus mutabilis, also known as confederate rose, is native to southeastern China, but it is also grown as an ornamental throughout the southeastern United States and is hardy in USDA zone 7 to 9. It is popular for its large, soft, gray-green foliage during the summer, and large, showy flowers pro...

  14. Cytotoxic Activity of the Leaf and Stem Extracts of Hibiscus rosa ...

    African Journals Online (AJOL)

    Methods: The crude petroleum ether, ethyl acetate and methanol extracts of the ... and stem of Hibiscus rosa sinensis were prepared using cold extraction method. ... (MTS) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide ...

  15. Kenaf (Hibiscus cannabinus L.) impact on post-germination seedling growth

    Science.gov (United States)

    The chemical interaction between plants, which is referred to as allelopathy, may result in the inhibition of plant growth and development. The objective of this research was to determine the impact of kenaf (Hibiscus cannabinus L.) plant extracts on the post-germination growth of five plant species...

  16. Antihypoxic and antioxidant activity of Hibiscus esculentus seeds

    Directory of Open Access Journals (Sweden)

    Eslami, Bahman

    2010-03-01

    Full Text Available The antihypoxic and antioxidant activities of Hibiscus esculentus seeds were investigated employing eight in vitro assay systems. Antihypoxic activity was investigated in two models, haemic and circulatory. The effects were pronounced in both models of hypoxia. The antihypoxic effects were dose-dependent. The results indicated that the extracts have a protective effect against hypoxia induced lethality in mice. The extracts showed antioxidant activity in some models. IC50 for DPPH radical-scavenging activity was 234 ± 8.9 μg ml-1. The extracts showed weak nitric oxide-scavenging activity between 0.1 and 1.6 mg ml-1. The extracts showed weak Fe2+ chelating ability. IC50 were 150 ± 13 μg ml-1. The extracts also exhibited low antioxidant activity in the linoleic acid model but were capable of scavenging hydrogen peroxide in a concentration dependent manner. The total amount of phenolic compounds in each extract was determined as gallic acid equivalents and total flavonoid contents were calculated as quercetin equivalents from a calibration curve. Pharmacological effects may be attributed, at least in part, to the presence of phenols and flavonoids in the extracts.La actividad antihipóxica y antioxidante de semillas de Hibiscus esculentus fue investigada empleando ocho ensayos in vitro. La actividad antihipóxica fue investigada en dos modelos, uno de caracter hemínico y otro circulatorio. Los efectos fueron pronunciados en ambos modelos de hipoxia. Los efectos antihipóxicos fueron dependientes de la dosis. Los resultados indican que los extractos tienen un efecto protector contra la letabilidad inducida por hipoxia en ratones. Los extractos mostraron actividad antioxidante en algunos modelos. El IC50 para la actividad captadora de radicales fue 234 ± 8.9 μg ml-1. Los extractos muestran una débil actividad captadora de óxido nítrico comprendida entre 0.1 y 1.6 mg ml-1. Los extractos muestran una débil capacidad quelatante de Fe2+. El IC

  17. INDIKATOR TITRASI ASAM-BASA DARI EKSTRAK BUNGA SEPATU (Hibiscus rosa sinensis L Indicator of Acid-Base Titration from the Extract of Hibiscus rosa sinensis L Flower

    Directory of Open Access Journals (Sweden)

    Siti Nuryanti

    2012-05-01

    Full Text Available Titration acid-base needs indicator  to show the change of color on interval of hydrogen exponent/degree of acid (pH. Indicator of synthetic which always be used have disadvantages like chemical pollution,  stock and expensive of pro- duction  cost. The research has been carried out to substitute the synthetic indicator with herbal indicator extracted from flower crown of Hibiscus rosa sinensis L extract. The herbal indicator was extracted from the flower crown Hibiscus rosa sinensis L using a mixture methanol-acetic acid. Then it was evaluated with phenolphthalein and methyl orange(E merck comparer to titration the acid-base, they are strong acid-strong base, weak base-strong acid and weak acid-strong base. The result of research show that herbal indicator  of flower crown Hibiscus rosa sinensis L to show theequivalent point in all titrations give peer result with the comparison. With the research result hoped that indicatortitration acid-base flower crown Hibiscus rosa sinensis L is able to as replace synthetic indicator  (metyl orange andphenolphtalein which always be used before. ABSTRAK Titrasi asam-basa memerlukan indikator untuk menunjukkan perubahan warna pada setiap interval derajad keasaman (pH. Indikator sintetis yang digunakan selama ini mempunyai beberapa kelemahan seperti polusi kimia, ketersediaan dan biaya produksi mahal. Upaya penelitian sudah dilakukan untuk menggantikan indikator sintetis dengan indikator dari ekstrak mahkota bunga sepatu. Indikator herbal tersebut dibuat dengan cara mengekstrak mahkota bunga Hibiscus rosa sinensis L dengan mengunakan pelarut metanol-asam asetat. Kemudian dievaluasi dengan indikator pembanding fenolftalein dan metil oranye (produksi E merck untuk titrasi asam-basa yaitu asam kuat-basa kuat, basa lemah-asam kuat dan asam lemah-basa kuat. Dari hasil penelitian diketahui bahwa indikator dari mahkota bunga sepatu untuk menunjukkan titik ekivalen dalam titrasi tersebut memberikan hasil yang

  18. Phytochemical analysis of Hibiscus caesius using high performance liquid chromatography coupled with mass spectrometry.

    Science.gov (United States)

    Ain, Quratul; Naveed, Muhammad Na; Mumtaz, Abdul Samad; Farman, Muhammad; Ahmed, Iftikhar; Khalid, Nauman

    2015-09-01

    Various species in genus Hibiscus are traditionally known for their therapeutic attributes. The present study focused on the phytochemical analysis of a rather unexplored species Hibiscus caesius (H. caesius), using high-pressure liquid chromatography coupled with mass spectrometry (HPLC-MS). The analysis revealed five major compounds in the aqueous extract, viz. vanillic acid, protocatechoic acid, quercetin, quercetin glucoside and apigenin, being reported for the first time in H. caesius. Literature suggests that these compounds have important pharmacological traits such as anti-cancer, anti-inflammatory, anti-bacterial and hepatoprotective etc. however, this requires further pharmacological investigations at in vitro and in vivo scale. The above study concluded the medicinal potential of H. caesius.

  19. Anthocyanin extracted from Hibiscus (Hibiscus rosa sinensis L.) as a photosensitizer on nanostructured-TiO2 dye sensitized solar cells

    Science.gov (United States)

    Ramelan, A. H.; Wahyuningsih, S.; Rosyida, N. A.; Supriyanto, E.; Saputro, S.; Hanif, Q. A.; Rinawati, L.

    2016-02-01

    Anthocyanin extracted from Hibiscus (Hibiscus rosa sinensis L) as a photosensitizer in nanostructured-TiO2 dye sensitized solar cells has been fabricated. Ultravisible visible absorption spectra of anthocyanin show an ability absorbing light in the visible region. While the nanostructed-TiO2 powder in this research was prepared by sol-gel method following annealled at a temperature of 600°C. Subsequently, the TiO2 nanostructures were characterized by XRD, XRF, and SEM. The difractogram X-ray results shown that TiO2 was built from f anatase and rutile phase. Element analysis of synthesized TiO2 by X-ray Fluorecence (XRF) shown the TiO2 content of 98,67 wt%. TiO2 layer prepared at different thickness showed the average size of cavity about 0.83 µm. These several thickness of solar cells were fabricated and were immersed into anthocyanin for 24 hours to gain sensitized TiO2 photoanode for Dye sensitised solar cells (DSSCs). These DSSCS performance were measured using I-V Keithley 2602A. The results exhibited that the sample with a TiO2 layer thickness of 4.75 ± 0.8 µm has the highest efficiency.

  20. Cardioprotective effect of the Hibiscus rosa sinensis flowers in an oxidative stress model of myocardial ischemic reperfusion injury in rat

    Directory of Open Access Journals (Sweden)

    Krishnamoorthy Karthikeyan K

    2006-09-01

    Full Text Available Abstract Background The present study investigates the cardioprotective effects of Hibiscus rosa sinensis in myocardial ischemic reperfusion injury, particularly in terms of its antioxidant effects. Methods The medicinal values of the flowers of Hibiscus rosa sinensis (Chinese rose have been mentioned in ancient literature as useful in disorders of the heart. Dried pulverized flower of Hibiscus rosa sinensis was administered orally to Wistar albino rats (150–200 gms in three different doses [125, 250 and 500 mg/kg in 2% carboxy methyl cellulose (CMC], 6 days per week for 4 weeks. Thereafter, rats were sacrificed; either for the determination of baseline changes in cardiac endogenous antioxidants [superoxide dismutase, reduced glutathione and catalase] or the hearts were subjected to isoproterenol induced myocardial necrosis. Results There was significant increase in the baseline contents of thiobarbituric acid reactive substances (TBARS [a measure of lipid per oxidation] with both doses of Hibiscus Rosa sinensis. In the 250 mg/kg treated group, there was significant increase in superoxide dismutase, reduced glutathione, and catalase levels but not in the 125 and 500 mg/kg treated groups. Significant rise in myocardial thiobarbituric acid reactive substances and loss of superoxide dismutase, catalase and reduced glutathione (suggestive of increased oxidative stress occurred in the vehicle treated hearts subjected to in vivo myocardial ischemic reperfusion injury. Conclusion It may be concluded that flower of Hibiscus rosa sinensis (250 mg/kg augments endogenous antioxidant compounds of rat heart and also prevents the myocardium from isoproterenol induced myocardial injury.

  1. Mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking Hibiscus rosa-sinensis L. in Malaysia, with two new country records

    Science.gov (United States)

    Sartiami, Dewi; Watson, Gillian W.; Mohamad Roff, M. N.; Idris, A. B.

    2016-11-01

    A survey of mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking the national flower of Malaysia, Hibiscus rosa-sisnensis L. and Hibiscus spp. (Malvaceae) was conducted in Selangor and Kuala Lumpur, Malaysia, from January to March 2016. Adult females were mounted on microscope slides in Canada balsam. The five species identified were Ferrisia dasylirii (Cockerell), Maconellicoccus hirsutus (Green), Paracoccus marginatus Williams and Granara de Willink, Phenacoccus solenopsis (Tinsley) and Pseudococcus jackbeardsleyi Gimpel & Miller. Two of these, the invasive species Ferrisia dasylirii and P. solenopsis were introduced and first recorded in Malaysia.

  2. Effect of different processing techniques on Indonesian Roselle (Hibiscus radiates seed constituents

    Directory of Open Access Journals (Sweden)

    Abdalbasit Adam Mariod

    2013-12-01

    Full Text Available   Background. Roselle seeds are waste that is left behind during processing of roselle for juices or other roselle related products. Disposing of waste is highly undesirable both economically and environmentally. Roselle seeds contain high amount of protein, crude fibers, fats, and carbohydrates.The objective of this study was to determine the effect of different processing techniques  (roasting using oven/microwave, and boiling on In- donesian roselle (Hibiscus radiates seed constituents. Methods.Three treatments were carried out to prepare the samples:The seeds were roasted at 130 and 150°C for 30 min (HOR, microwave roasting for 10, 20 and 30 min HMR10, HMR20, HMR30 of roselle seeds. Hibiscus seeds (3×200 g were put into boiling tap water (100°C (HB in a 500 ml beaker on magnetic stirred hot plate at a ratio of 1:4 seed: water for 40 min until the pieces were  well cooked and tender. Proximate chemical analysis was determined following the standard methods of the Association of Official Analytical Chemists. Hibiscus different samples were analysed in triplicate and the results were reported as means. Total  carbohydrate content was calculated from the difference. The fatty acids of the oil samples were analysed using gas chromatography (Shimadzu, GC-2010A series, Shimadzu, Tokyo, Japan equipped with a flame ionization detector and a BPX70 capillary column of  30 m × 0.32 mm i.d. (SGE, Melbourne, Australia. The tocopherol content of the oil samples was measured by HPLC (Cecil  Instruments Ltd., Cambridge, England. Results.  Proximate composition of untreated, roasted and boiled hibiscus seeds showed that, roasting and boiling temperatures can increase fat and fiber content, microwave and boiling showed higher fat content when compared with oven roasting treatment. Protein content of HU was significantly lower (p HMR > HOR. Protein content of HB, HMR and HOR increased significantly as com- pared with HU. The carbohydrate values of

  3. Multi-locus DNA barcoding identifies matK as a suitable marker for species identification in Hibiscus L.

    Science.gov (United States)

    Poovitha, Sundar; Stalin, Nithaniyal; Balaji, Raju; Parani, Madasamy

    2016-12-01

    The genus Hibiscus L. includes several taxa of medicinal value and species used for the extraction of natural dyes. These applications require the use of authentic plant materials. DNA barcoding is a molecular method for species identification, which helps in reliable authentication by using one or more DNA barcode marker. In this study, we have collected 44 accessions, representing 16 species of Hibiscus, distributed in the southern peninsular India, to evaluate the discriminatory power of the two core barcodes rbcLa and matK together with the suggested additional regions trnH-psbA and ITS2. No intraspecies divergence was observed among the accessions studied. Interspecies divergence was 0%-9.6% with individual markers, which increased to 0%-12.5% and 0.8%-20.3% when using two- and three-marker combinations, respectively. Differentiation of all the species of Hibiscus was possible with the matK DNA barcode marker. Also, in two-marker combinations, only those combinations with matK differentiated all the species. Though all the three-marker combinations showed 100% species differentiation, species resolution was consistently better when the matK marker formed part of the combination. These results clearly showed that matK is more suitable when compared to rbcLa, trnH-psbA, and ITS2 for species identification in Hibiscus.

  4. Investigation of the bacterial retting community of kenaf (Hibiscus cannabinus) under different conditions using next-generation semiconductor sequencing

    Science.gov (United States)

    The use of the natural fibers requires the development of cost-efficient processing of fibers with consistent, uniform properties. The microbial communities associated with kenaf (Hibiscus cannabinus) plant fibers during retting were determined in an effort to identify possible means of accelerating...

  5. The Role of Leaves in Photocontrol of Flower Bud Abscission in Hibiscus rosa-sinensis L. 'Nairobi'

    NARCIS (Netherlands)

    Meeteren, van U.; Gelder, van A.

    2000-01-01

    When compared with exposure to darkness, exposing Hibiscus rosa-sinensis L. 'Nairobi' plants to red light (635 to 685 nm, 2.9 μmol?m-2?s-1) delayed flower bud abscission, while exposure to far-red light (705 to 755 nm, μmol?m-2?s-1) accelerated this process. Flower bud abscission in response to ligh

  6. Wilt, crown, and root rot of common rose mallow (Hibiscus moscheutos) caused by a novel Fusarium sp.

    Science.gov (United States)

    A new crown and root rot disease of landscape plantings of the malvaceous ornamental common rose mallow (Hibiscus moscheutos) was first detected in Washington State in 2012. The main objectives of this study were to identify the causal agent using multilocus molecular phylogenetics and to complete K...

  7. Growth and biomass productivity of kenaf (Hibiscus cannabinus, L.) under different agricultural inputs and management practices in central Greece

    NARCIS (Netherlands)

    Danalatos, N.G.; Archontoulis, S.V.

    2010-01-01

    The growth and biomass productivity of kenaf (Hibiscus cannabinus, L.) cultivars Tainung 2 and Everglades 41 were determined under three irrigation applications (low: 25%, moderate: 50% and fully: 100% of maximum evapotranspiration; ETm), four nitrogen dressings (0, 50, 100 and 150 kg hat), two sowi

  8. The Role of Leaves in Photocontrol of Flower Bud Abscission in Hibiscus rosa-sinensis L. 'Nairobi'

    NARCIS (Netherlands)

    Meeteren, van U.; Gelder, van A.

    2000-01-01

    When compared with exposure to darkness, exposing Hibiscus rosa-sinensis L. 'Nairobi' plants to red light (635 to 685 nm, 2.9 μmol?m-2?s-1) delayed flower bud abscission, while exposure to far-red light (705 to 755 nm, μmol?m-2?s-1) accelerated this process. Flower bud abscission in response to

  9. Molecular cloning, characterization and expression of the caffeic acid O-methyltransferase (COMT) ortholog from kenaf (Hibiscus cannabinus)

    Science.gov (United States)

    We cloned the full-length of the gene putatively encoding caffeic acid O-methyltransferase (COMT) from kenaf (Hibiscus cannabinus L.) using degenerate primers and the RACE (rapid amplification of cDNA ends) method. Kenaf is an herbaceous and rapidly growing dicotyledonous plant with great potential ...

  10. Impact of kenaf (Hibiscus cannabinus L.) leaf, bark, and core extracts on germination of five plant species

    Science.gov (United States)

    The chemical interaction between plants, which is referred to as allelopathy, may result in the inhibition of plant growth and development. The objective of this research was to determine the impact of kenaf (Hibiscus cannabinus L.) plant extracts on the germination and post-germination development ...

  11. Chemical-sensory properties and consumer preference of hibiscus beverages produced by improved industrial processes.

    Science.gov (United States)

    Monteiro, Maria João P; Costa, Ana Isabel A; Fliedel, Geneviève; Cissé, Mady; Bechoff, Aurélie; Pallet, Dominique; Tomlins, Keith; Pintado, Maria Manuela E

    2017-06-15

    The need to increase sustainability and add value to traditional foods claiming health benefits led to the introduction of key improvements in the production of hibiscus beverages in Senegal. The physicochemical and sensory properties of three resulting products (an under-vacuum concentrate, a dilute-to-taste syrup and a ready-to-drink infusion) were assessed, vis-à-vis those of conventionally manufactured beverages, and their impact on local consumer preference determined (n=146). New beverages had more intense, redder colour and higher monomeric anthocyanin content, total phenolic content and antioxidant capacity. Moreover, their colour evaluations by trained panellists were mainly linked to colour density and anthocyanin/polyphenol content, while flavour assessments were associated to titratable acidity and sugar-to-acid ratio. Consumer evaluations, in turn, were driven by the beverages' red colour intensity, aroma strength and balance between sweetness and acidity. This explained why they overwhelmingly preferred the under-vacuum concentrate, regardless of their age, gender or frequency of hibiscus beverage consumption. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Antidepressant-like effects of methanol extract of Hibiscus tiliaceus flowers in mice

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    Vanzella Cláudia

    2012-04-01

    Full Text Available Abstract Background Hibiscus tiliaceus L. (Malvaceae is used in postpartum disorders. Our purpose was to examine the antidepressant, anxiolytic and sedative actions of the methanol extract of H. tiliaceus flowers using animal models. Methods Adult male Swiss albino mice were treated with saline, standard drugs or methanol extract of H. tiliaceus and then subjected to behavioral tests. The forced swimming and tail suspension tests were used as predictive animal models of antidepressant activity, where the time of immobility was considered. The animals were submitted to the elevated plus-maze and ketamine-induced sleeping time to assess anxiolytic and sedative activities, respectively. Results Methanol extract of H. tiliaceus significantly decreased the duration of immobility in both animal models of antidepressant activity, forced swimming and tail suspension tests. This extract did not potentiate the effect of ketamine-induced hypnosis, as determined by the time to onset and duration of sleeping time. Conclusion Our results indicate an antidepressant-like profile of action for the extract of Hibiscus tiliaceus without sedative side effect.

  13. Laboratory Evaluation of Different Insecticides against Hibiscus Mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Fatima, Samman; Hussain, Mubashar; Shafqat, Shama; Faheem Malik, Muhammad; Abbas, Zaheer; Noureen, Nadia; Ul Ane, Noor

    2016-01-01

    Hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae), is the major pest of many vegetables, fruits, crops, and ornamental plants causing losses to the farmers and its control has been an issue of significance in the pest management. This study was aimed at evaluating different concentrations (0.06%, 0.1%, and 0.14%) of Telsta, Advantage, Talstar, Imidacloprid, and their mixtures against hibiscus mealybug in the Laboratory of Systematics and Pest Management at University of Gujrat, Pakistan. The toxic effect was evaluated in the laboratory bioassay after 24 and 48 h of the application of insecticides. The highest mortality (95.83%) was shown by Talstar and Talstar + Imidacloprid at the concentration of 0.14% after 48 h followed by Advantage + Talstar with 87.50% mortality at 0.14% concentration after 48 h of application. The study also showed that the least effective treatment observed was Advantage + Telsta with no mortality after 24 h and 25% mortality after 48 h at 0.14% concentration. The study revealed that the concentration 0.14% was highly effective in lowering the mealybug population and insecticide mixtures were effective in reducing mealybug density. The study emphasizes the use of such insecticide mixtures to develop better management strategy for mealybug populations attacking ornamental plants. However effects of such insecticide mixtures on other organisms and biological control agents should be checked under field conditions.

  14. Cytotoxic activity of kenaf (Hibiscus cannabinus L.) seed extract and oil against human cancer cell lines

    Institute of Scientific and Technical Information of China (English)

    Yu Hua Wong; Wai Yan Tan; Chin Ping Tan; Kamariah Long; Kar Lin Nyam

    2014-01-01

    Objective: To examine the cytotoxic properties of both the kenaf (Hibiscus cannabinus L.) seed extract and kenaf seed oil on human cervical cancer, human breast cancer, human colon cancer and human lung cancer cell lines.Methods:kenaf seed oil on human cancer cell lines was evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and sulforhodamine B assays. Cell morphological changes were observed by using an inverted light microscope.Results:The in vitro cytotoxic activity of the kenaf (Hibiscus cannabinus L.) seed extract and cancer cell lines. Morphological alterations in the cell lines after KSE and KSO treatment were observed. KSE and KSO possessed effective cytotoxic activities against all the cell lines been selected.Conclusions:KSE and KSO could be potential sources of natural anti-cancer agents. Further The kenaf seed extract (KSE) exhibited a lower IC50 than kenaf seed oil (KSO) in all of the investigations on using kenaf seeds for anti-proliferative properties are warranted.

  15. Laboratory Evaluation of Different Insecticides against Hibiscus Mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae

    Directory of Open Access Journals (Sweden)

    Samman Fatima

    2016-01-01

    Full Text Available Hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae, is the major pest of many vegetables, fruits, crops, and ornamental plants causing losses to the farmers and its control has been an issue of significance in the pest management. This study was aimed at evaluating different concentrations (0.06%, 0.1%, and 0.14% of Telsta, Advantage, Talstar, Imidacloprid, and their mixtures against hibiscus mealybug in the Laboratory of Systematics and Pest Management at University of Gujrat, Pakistan. The toxic effect was evaluated in the laboratory bioassay after 24 and 48 h of the application of insecticides. The highest mortality (95.83% was shown by Talstar and Talstar + Imidacloprid at the concentration of 0.14% after 48 h followed by Advantage + Talstar with 87.50% mortality at 0.14% concentration after 48 h of application. The study also showed that the least effective treatment observed was Advantage + Telsta with no mortality after 24 h and 25% mortality after 48 h at 0.14% concentration. The study revealed that the concentration 0.14% was highly effective in lowering the mealybug population and insecticide mixtures were effective in reducing mealybug density. The study emphasizes the use of such insecticide mixtures to develop better management strategy for mealybug populations attacking ornamental plants. However effects of such insecticide mixtures on other organisms and biological control agents should be checked under field conditions.

  16. Determination of Suitable Microspore Stage and Callus Induction from Anthers of Kenaf (Hibiscus cannabinus L.

    Directory of Open Access Journals (Sweden)

    Ahmed Mahmood Ibrahim

    2014-01-01

    Full Text Available Kenaf (Hibiscus cannabinus L. is one of the important species of Hibiscus cultivated for fiber. Availability of homozygous parent lines is prerequisite to the use of the heterosis effect reproducible in hybrid breeding. The production of haploid plants by anther culture followed by chromosome doubling can be achieved in short period compared with inbred lines by conventional method that requires self pollination of parent material. In this research, the effects of the microspore developmental stage, time of flower collection, various pretreatments, different combinations of hormones, and culture condition on anther culture of KB6 variety of Kenaf were studied. Young flower buds with immature anthers at the appropriate stage of microspore development were sterilized and the anthers were carefully dissected from the flower buds and subjected to various pretreatments and different combinations of hormones like NAA, 2,4-D, Kinetin, BAP, and TDZ to induce callus. The best microspore development stage of the flower buds was about 6–8 mm long collected 1-2 weeks after flower initiation. At that stage, the microspores were at the uninucleate stage which was suitable for culture. The best callus induction frequency was 90% in the optimized semisolid MS medium fortified with 3.0 mg/L BAP + 3.0 mg/L NAA.

  17. ANTI IMPLANTATION AND PREGNANCY INTERRUPTION ACTIVITY OF JAPAKUSUMA (HIBISCUS ROSA SINENSIS IN ALBINO RATS

    Directory of Open Access Journals (Sweden)

    Kashinath Hadimur

    2013-06-01

    Full Text Available Increase in population has affected many socio-economic conditions of people by increasing crimes, illiteracy, destructive activities, diseases, improper food and shelter. Thus to control this population and limit the family size at a personal level and at a national level, modern contraceptive methods and medicines were introduced long back. There are many new contraceptives available now, but they have various side effects. Some traditional practitioners used to dispense oral contraceptives mentioned in Ayurvedic classics. Such as 1 Pippali (Piper longum, Vidanga (Emblica ribes and Tankana (Sodii Biboras. 2 Talisapatra (Taxus baccata and Gairika (Hematite with cold water and 3 Kanji bhavita Japakusuma (Hibiscus rosa sinensis. An experimental study on above mentioned 1 and 2 formulations has proved its efficacy as temporary contraceptive medicine. To evaluate the permanent or long term temporary contraceptive effect of Japakusuma, an attempt was made in this study. Study was conducted by Choudary and Khanna method on 18 female, 36 male (for mating albino rats. Japakusuma, Propylene glycol, Ovral L formed the materials. Single dose was administered on proestrous stage of rat oestrous cycle and observed for anti-implantation and pregnancy interruption activity. Test drug showed significant anti implantation and pregnancy interruption activity. Thus showing the temporary contraceptive activity of Japakusuma (Hibiscus rosa sinensis.

  18. Chemical constituents from Hibiscus tiliaceus%黄槿化学成分的研究

    Institute of Scientific and Technical Information of China (English)

    张小坡; 张俊清; 裴月湖; 许旭东; 谭银丰; 康胜利; 刘明生

    2012-01-01

    目的 研究黄槿Hibiscus tiliaceus枝叶的化学成分.方法 采用硅胶柱色谱、Sephadex LH-20柱色谱分离化合物,并通过理化数据测定和波谱技术分析鉴定化合物的结构.结果 从黄槿枝叶70%乙醇提取物中分离得到14个化合物,分别鉴定为木栓酮(1)、β-谷甾醇(2)、香草醛(3)、syriacusinA(4)、hibiscolactone (5)、莨菪亭(6)、臭矢菜素C(7)、反式丁烯二酸(8)、山柰酚(9)、槲皮素(10)、胡萝卜苷(11)、壬二酸(12)、丁二酸(13)、芦丁 (14).结论 化合物7、9、10为首次从该属植物中分离得到,化合物3~5、8、12~14为首次从该植物中分离得到.%Objective To study the chemical constituents from the twigs and leaves of Hibiscus tiliaceus (Li medicine). Methods Compounds were isolated by repeated chromatography on silica gel and alumina gel column. Their structures were elucidated by chemical and spectroscopic methods. Results Fourteen compounds were isolated and identified as friedelin (1), fi-sitosterol (2), vanillin (3), syriacusin A (4), hibiscolactone (5), scopoletin (6), cleomiscosin C (7), fiimaric acid (8), kaempferol (9), quercetin (10), daucosterol (11), azelaic acid (12), succinic acid (13), and rutin (14). Conclusion This is the first report on the isolation of compounds 7, 9, and 10 from the plants of Hibiscus Linn., and compounds 3-5,8, and 12-14 from this plant.

  19. Synthesis of monodispersed silver nanoparticles using Hibiscus cannabinus leaf extract and its antimicrobial activity

    Science.gov (United States)

    Bindhu, M. R.; Umadevi, M.

    2013-01-01

    Synthesis of silver nanoparticles using leaf extract of Hibiscus cannabinus has been investigated. The influences of different concentration of H. cannabinus leaf extract, different metal ion concentration and different reaction time on the above cases on the synthesis of nanoparticles were evaluated. The synthesized nanoparticles were characterized using UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Transmission Electron Microscopy (TEM). The prepared silver nanoparticles were monodispersed, spherical in shape with the average particle size of 9 nm and shows surface plasmon peak at 446 nm. The study also reveals that the ascorbic acid present in H. cannabinus leaf extract has been used as reducing agent. The prepared silver nanoparticle shows good antimicrobial activity against Escherichia coli, Proteus mirabilis and Shigella flexneri.

  20. STUDI VARIASI UKURAN SERBUK SARI KEMBANG SEPATU (HIBISCUS ROSA-SINENSIS L. DENGAN WARNA BUNGA BERBEDA

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    NI MADE DENNI APRIANTY

    2008-06-01

    Full Text Available The purpose of this study conducted to know variety of : long of axis polar, equatorial plane diameters, and P/E index of ten Kembang Sepatu (Hibiscus rosa-sinensis L. with differnt colour flowers. Acetolysis method and 1 % Safranin staining used to make pollens slides, micrometri were used to measured long of polar axis and diameter of pollens. The result of this research showed : pollen structure have: 90,10 ± 3,02 ^im to 117,42 ± 1,37 l^m axis polar long, 89,66 ± 3,13 |^m to 112,92 ± 0,94 (xm equatorial plane diameter, and 0,99 to 1,04 P/E index. Based on these data is indicated that the type of the pollen are prolat sferoidal (except bud type red corolla, and white flower polypantoporat type appertura and periporat ornamentation exine .

  1. Cytotoxic effect of triterpenoids from the root bark of Hibiscus syriacus.

    Science.gov (United States)

    Shi, Li-Shian; Wu, Chao-Hsuan; Yang, Te-Chun; Yao, Chen-Wen; Lin, Hang-Ching; Chang, Wen-Liang

    2014-09-01

    In this study, 4 new triterpenoids-3β- acetoxy-olean-11-en,28,13β-olide (1), 3β- acetoxy-11α,12α-epoxy-olean-28,13β-olide (2), 19α-epi-betulin (3), and 20, 28-epoxy-17β,19β-lupan-3β-ol (4)-and 12 known compounds, were isolated from the root bark of Hibiscus syriacus L. by using acetone extraction. Their structures were characterized by extensive spectroscopic analysis. To investigate cytotoxicity, A549 human lung cancer cells were exposed to the extract and the compounds identified from it. Significantly reduced cell viability was observed with betulin-3-caffeate (12) (IC50, 4.3 μM). The results of this study indicate that betulin-3-caffeate (12) identified from H. syriacus L. may warrant further investigation for potential as anticancer therapies.

  2. Antioxidant and hepatoprotective effect of the roots of Hibiscus esculentus Linn.

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    Sunilson J

    2008-01-01

    Full Text Available The ethanol extract of Hibiscus esculentus Linn roots inhibited the formation of oxygen derived free radicals invitro with IC 50 of 620 µg/ml, 2300 µg/ml, 870 µg/ml and 610 µg/ml. [Fe 2+ /ascobate system], 730 µg/ml, [Fe 3+ /ADP/ascorbate system], for superoxide radical production, hydroxyl radical generation, nitric oxide radical formation and lipid peroxide formation respectively. The oral administration of the extract (250 and 500 mg/kg body weight, significantly reduce CCl 4 induced hepatotoxicity in rats, as judged from the serum and tissue activity of marker enzymes [Glutamate oxaloacetate transaminase (GOT, Glutamate pyruvate transaminase (GPT and alkaline phosphatase (ALP]. These results were comparable with standard drug silymarin [20 mg/kg, P.O.

  3. Pollen and Anther Cultures as Potential Means in Production of Haploid Kenaf (Hibiscus cannabinus L.

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    Dwi Susanto

    2013-01-01

    Full Text Available Kenaf (Hibiscus cannabinus L. is one of the important species of Hibiscus for human needs. It is annual upright plant that produces high biomass, has good adaptability to its environment and inexpensive to be grown. Kenaf has been cultivated in many countries since long time ago mainly for fibers. In Malaysia, however, this plant is considered new and cultivated especially in the eastern parts of Peninsular to replace tobacco plantations that have been no longer supported by the government. This plant has potential to be bred to improve the quality and the adaptability. One of the potential breeding methods to improve the plant is by the production of hybrid varieties. These varieties are superior over the inbred varieties because of the heterosis effect (hybrid vigor ofF1heterozygous. Hybrid varieties can be produced by reciprocal combinations of crosses among homozygous lines. Homozygous lines conventionally can bachieved by repeated selfing till seven or more generations that need a lot of times. The production of haploid plants in vitro followed by chromosome doubling is a ‘short-cut’ method to produce homozygous lines in relatively short time compared to conventional methods. The objective of this study is to produce haploid lines through pollen and anther cultures in vitro. The population of haploid plantlets from this research will be induced for diploidization to produce homozygous-heterogeneous population as a basis for the production of hybrid varieties. Preliminary result showed that callus can easily be induced from the anthers compared those from the pollen grains.

  4. Development and evaluation of Ketoprofen sustained release matrix tablet using Hibiscus rosa-sinensis leaves mucilage

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    M. Kaleemullah

    2017-07-01

    Full Text Available Currently, the use of natural gums and mucilage is of increasing importance in pharmaceutical formulations as valuable drug excipient. Natural plant-based materials are economic, free of side effects, biocompatible and biodegradable. Therefore, Ketoprofen matrix tablets were formulated by employing Hibiscus rosa-sinensis leaves mucilage as natural polymer and HPMC (K100M as a synthetic polymer to sustain the drug release from matrix system. Direct compression method was used to develop sustained released matrix tablets. The formulated matrix tablets were evaluated in terms of physical appearance, weight variation, thickness, diameter, hardness, friability and in vitro drug release. The difference between the natural and synthetic polymers was investigated concurrently. Matrix tablets developed from each formulation passed all standard physical evaluation tests. The dissolution studies of formulated tablets revealed sustained drug release up to 24 h compared to the reference drug Apo Keto® SR tablets. The dissolution data later were fitted into kinetic models such as zero order equation, first order equation, Higuchi equation, Hixson Crowell equation and Korsmeyer-Peppas equation to study the release of drugs from each formulation. The best formulations were selected based on the similarity factor (f2 value of 50% and more. Through the research, it is found that by increasing the polymers concentration, the rate of drug release decreased for both natural and synthetic polymers. The best formulation was found to be F3 which contained 40% Hibiscus rosa-sinensis mucilage polymer and showed comparable dissolution profile to the reference drug with f2 value of 78.03%. The release kinetics of this formulation has shown to follow non-Fickian type which involved both diffusion and erosion mechanism. Additionally, the statistical results indicated that there was no significant difference (p > 0.05 between the F3 and reference drug in terms of MDT and

  5. Phytochemical, Physico-chemical & Spectroscopic Characteristics of Ethanolic Extract of Leaf, Stem and Flower bud of Hibiscus hispidissimus Griffith

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    Thamizh Selvam N

    2015-08-01

    Full Text Available The plant Hibiscus hispidissimus belongs to the family Malvaceae (Mallow family. The plant has wide range of medicinal uses. Considering the ethno medicinal value of Hibiscus hispidissimus, the present work has been taken up to document the physico-chemical composition, phytochemical details and spectrophotometric characteristics of the plant. The work has been carried out on ethanolic extract of leaf, stem and flower bud of H. hispidissimus. Phytochemical analysis showed the presence of saponis, tannins, glycosides, diterpenes and quinones. Spectroscopic characteristics were analyzed and found to have wide range of compounds including steroids, alkaloids, pigments like chlorophyll a and b, phenolic compounds mainly gallic acid, flavanoids like anthocyanins, flavanols, flvanones and isoflavones.

  6. Antioxidant and Antidiabetic Effect of Hibiscus rosasinensis Flower Extract on Streptozotocin Induced Experimental Rats-a Dose Response Study

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    Mirunalini SANKARAN

    2011-11-01

    Full Text Available The present study was designed to evaluate the antidiabetic and antioxidant effect of Hibiscus rosasinensis against streptozotocin induced diabetic rats. Streptozotocin (STZ was administered as a single dose (40 mg/kg to induce diabetes. The hypoglycemic activity of Hibiscus rosasinensis extract (HRSEt was investigated in a dose dependent manner such as (125, 250 and 500 mg/kg bwt by evaluating various biochemical parameters. The levels of blood glucose, carbohydrate metabolizing enzymes, TBARS, enzymatic and non-enzymatic antioxidants and lipid profiles were found to be significantly increased in diabetic rats when compared to control groups. Administration of extract in the treated groups showed altered changes in the above mentioned parameters and found that among the three doseses, 250 mg/kg showed best result when compared to other two doses. HRSEt possess antioxidant, hypoglycemic and hypolipidemic activity against streptozotocin induced diabetic rats. However the detailed mechanism(s of action will require elucidating in further studies.

  7. Acclimation of croton and hibiscus seedlings in response to the application of indobultiric acid and humic acid for rooting

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    Lílian Estrela Borges Baldotto

    2015-06-01

    Full Text Available The vegetative propagation of ornamental plants can be accelerated by applying plant growth regulators. Amongst them, the use of auxins, plant hormones with physiological effects on cell elongation and rooting have stood out. Alternatively, the application of humic acids, bioactive fraction of soil organic matter, also results in increases in rooting cuttings of ornamental plants. The objective of this work was to study the growth characteristics and the nutritional contents of croton and hibiscus plants during acclimation of seedlings in response to different concentrations of indolebutyric acid (IBA and humic acid (HA applied to cuttings for rooting. The experiment was conducted in greenhouse, and the apical stem cuttings were treated with solutions with concentrations of 0, 250, 500, 1000 and 2000 mg L-1of IBA and 0, 10, 20, 30 and 40 mg L-1 of C from HA. At 45 days of rooting in carbonized rice husk, they were individually transferred to plastic bags of 2.0 dm3 containing a mixture of soil: sand: manure (2: 1: 1 as substrate. At 90 days of acclimation, the plants were collected for measurement of growth and nutritional variables. The results showed that the application of the IBA stimulates the absorption of nutrients and growth of croton cuttings and transplanted hibiscus, contributing to formation of vigorous seedlings. A similar response occurred with the application of HA in hibiscus cuttings

  8. Effects of Basella alba and Hibiscus macranthus extracts on testosterone production of adult rat and bull Leydig cells

    Institute of Scientific and Technical Information of China (English)

    Paul F. Moundipa; Nathalie Sara E. Beboy; Fabien Zelefack; Silvère Ngouela; Etienne Tsamo; Wolf-Bernhard Schill; Thomas K. Monsees

    2005-01-01

    Aim: To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the bull,and to develop a novel in vitro test system using Leydig cells from bull testes. Methods: The effect of methanol extracts from both plants on testosterone production in isolated Leydig cells from the rat and the bull was analyzed using 125I-radioimmunoassay (125I-RIA). Rat Leydig cells were obtained by common methods, whereas a novel technique was used to purify Leydig cells from bull testes. Results: Bull testes from the slaughter house were a cheap source of pure Leydig cells. In culture, these cells produced testosterone for 5-6 days, which can be stimulated by human chorionic gonadotrophin (hCG). Basella alba extracts significantly enhanced testosterone production in bull and rat Leydig cells in a concentration-dependent manner. Hibiscus macranthus showed no androgenic effect but was shown to inhibit testosterone production at higher concentrations. Conclusion: Leydig cells purified from bull testes can be used as an alternative tool in experimental animal research. Certain fractions of Basella alba extract demonstrated androgenic potential whereas Hibiscus macranthus extracts did not.

  9. JMBR VOLUME 14 NO 1 - JUNE 2015.cdr- CORRESPONDENCE.cdr

    African Journals Online (AJOL)

    FinePrint

    JMBR: A Peer-review Journal of Biomedical Sciences. June 2015, Vol. 14 No ... EFFECT OF HIBISCUS SABDARIFFA CALYX EXTRACT ON. STRESSED ... intermediary hormone between the. 5 .... lipolysis in adipose tissue, through activation ...

  10. Download this PDF file

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    from a commonly available indigenous raw material, Hibiscus sabdariffa L. The said ... The microbial loads of the experimental tea and control were ..... treatment before packaging if it were to be ... reported that good preservation of canned.

  11. Fingerprint analysis of Hibiscus mutabilis L. leaves based on ultra performance liquid chromatography with photodiode array detector combined with similarity analysis and hierarchical clustering analysis methods

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    Xianrui Liang

    2013-01-01

    Full Text Available Background: A method for chemical fingerprint analysis of Hibiscus mutabilis L. leaves was developed based on ultra performance liquid chromatography with photodiode array detector (UPLC-PAD combined with similarity analysis (SA and hierarchical clustering analysis (HCA. Materials and Methods: 10 batches of Hibiscus mutabilis L. leaves samples were collected from different regions of China. UPLC-PAD was employed to collect chemical fingerprints of Hibiscus mutabilis L. leaves. Results: The relative standard deviations (RSDs of the relative retention times (RRT and relative peak areas (RPA of 10 characteristic peaks (one of them was identified as rutin in precision, repeatability and stability test were less than 3%, and the method of fingerprint analysis was validated to be suitable for the Hibiscus mutabilis L. leaves. Conclusions: The chromatographic fingerprints showed abundant diversity of chemical constituents qualitatively in the 10 batches of Hibiscus mutabilis L. leaves samples from different locations by similarity analysis on basis of calculating the correlation coefficients between each two fingerprints. Moreover, the HCA method clustered the samples into four classes, and the HCA dendrogram showed the close or distant relations among the 10 samples, which was consistent to the SA result to some extent.

  12. Estudio fitoquímico de la especie Hibiscus elatus S.W.

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    Ingrid Márquez Hernández

    1999-08-01

    Full Text Available Se realizó un estudio fitoquímico preliminar de la especie Hibiscus elatus S.W. Se practicó un tamizaje fitoquímico sobre material seco y fresco, en el que se obtuvieron resultados positivos para mucílagos, sustancias reductoras, antocianinas, aminoácidos, taninos y flavonoides. Se determinó que las antocianinas se afectan durante el proceso de secado. Para la realización del estudio químico se maceró el material vegetal con 4 menstruos (fracciones A, B, C y D. A partir de las fracciones A y B se aisló el producto HE1, el cual se analizó por espectroscopia ultravioleta, infrarroja, de resonancia magnética nuclear protónica, de carbono 13, mediante uso de técnicas especiales y por espectrometría de masas, lo que permitió identificarlo como el flavonoide gossypitrina. La fracción C se sometió a un fraccionamiento según el método de absorción-desorción sobre silicagel, lo que permitió la detección de rutina y quercetina. Los flavonoides identificados se reportan por primera vez para la especie.A preliminary phytochemical study of Hibiscus elatus species was undertaken. A phytochemical sieving of fresch dry material made it possible to obtain positive results for mucilages, reducing substances, anthocyamins, aminoacids, tanines and flavonoids. Anthocyamines were determined to be affected during dryng process. For carry ing uot the chemical study, the plant was mecrated into 4 solvents (A, B, C and D fractions. Taking A and B fractions as a basis, product HE1 was isolated and analyzed by ultra - violet, infrared, nuclear magnetic resonance, proton and carbon 13 spectroscopies as well as special techniques and mass spectrometry. This resulted in the identification of this product as gossypitrin flavonoid. Fraction C was fractioned by silicagel absorption - desorption methods which allowed us to detec rutin and quercetin. The identified flavonoids are reported for the first time in this species.

  13. Hemidesmus indicus and Hibiscus rosa-sinensis Affect Ischemia Reperfusion Injury in Isolated Rat Hearts

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    Vinoth Kumar Megraj Khandelwal

    2011-01-01

    Full Text Available Hemidesmus indicus (L. R. Br. (HI and Hibiscus rosa-sinensis L. (HRS are widely used traditional medicine. We investigated cardioprotective effects of these plants applied for 15 min at concentrations of 90, 180, and 360 μg/mL in Langendorff-perfused rat hearts prior to 25-min global ischemia/120-min reperfusion (I/R. Functional recovery (left ventricular developed pressure—LVDP, and rate of development of pressure, reperfusion arrhythmias, and infarct size (TTC staining served as the endpoints. A transient increase in LVDP (32%–75% occurred at all concentrations of HI, while coronary flow (CF was significantly increased after HI 180 and 360. Only a moderate increase in LVDP (21% and 55% and a tendency to increase CF was observed at HRS 180 and 360. HI and HRS at 180 and 360 significantly improved postischemic recovery of LVDP. Both the drugs dose-dependently reduced the numbers of ectopic beats and duration of ventricular tachycardia. The size of infarction was significantly decreased by HI 360, while HRS significantly reduced the infarct size at all concentrations in a dose-dependent manner. Thus, it can be concluded that HI might cause vasodilation, positive inotropic effect, and cardioprotection, while HRS might cause these effects at higher concentrations. However, further study is needed to elucidate the exact mechanism of their actions.

  14. Germination biology of Hibiscus tridactylites in Australia and the implications for weed management

    Science.gov (United States)

    Chauhan, Bhagirath Singh

    2016-05-01

    Hibiscus tridactylites is a problematic broadleaf weed in many crops in Australia; however, very limited information is available on seed germination biology of Australian populations. Experiments were conducted to evaluate the effect of environmental factors on germination and emergence of H. tridactylites. Germination was stimulated by seed scarification, suggesting the inhibition of germination in this species is mainly due to the hard seed coat. Germination was not affected by light conditions, suggesting that seeds of this species are not photoblastic. Germination was higher at alternating day/night temperatures of 30/20 °C (74%) and 35/25 °C (69%) than at 25/15 °C (63%). Moderate salinity and water stress did not inhibit germination of H. tridactylites. Seedling emergence of H. tridactylites was highest (57%) for the seeds buried at a 2 cm depth in the soil; 18% of seedlings emerged from seeds buried at 8 cm but no seedlings emerged below this depth. Soil inversion by tillage to bury weed seeds below their maximum depth of emergence could serve an important tool for managing H. tridactylites.

  15. EFFECTS OF ALKALINE PRE-IMPREGNATION AND PULPING ON MALAYSIA CULTIVATED KENAF (HIBISCUS CANNABINUS

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    Lin Suan Ang

    2010-05-01

    Full Text Available This study was carried out to identify an appropriate alkaline pulping condition for Malaysia cultivated kenaf (Hibiscus cannabinus L.. The chemical composition of the kenaf bast and core fibers, and also whole stalk with different growing time were examined prior to pulping attempts. The results of various soda-AQ pulping showed that the degree of carbohydrate degradation and delignification increased with the increase of active alkali and cooking temperature, but decreased with the increase of liquor to material (L:M ratio. The most satisfactory properties of pulp and handsheets from bast could be attained by employing soda-AQ pulping with 19.4% active alkali, 0.10% AQ, and L:M ratio of 7:1 cooked for 2 hours at 160˚C. Besides, it was also found that a mild alkaline pre-impregnation prior pulping improved the pulp viscosity and handsheets’ strength properties, especially the tensile index and folding endurance effectively. Moreover, among the three alkaline pulping processes—kraft, kraft-AQ, and soda-AQ—the results of pulp and handsheet properties showed that the soda-AQ pulp was comparable or even slightly of higher quality than the kraft pulps. Between the unbeaten bast and core soda-AQ handsheets, the strength properties of the core were higher than the bast, as the thin-walled core fibers exhibited much better conformability than the thick-walled bast fibers.

  16. In vitro antibacterial activity of Hibiscus rosa-sinensis flower extract against human pathogens

    Institute of Scientific and Technical Information of China (English)

    Ruban P; Gajalakshmi K

    2012-01-01

    Objective: To access the in vitro antibacterial activity of Hibiscus rosa-sinensis (H. rosa- sinensis) flower extract against human pathogens. Methods: Antibacterial activity was evaluated by using disc and agar diffusion methods. The protein was run through poly acrylmide gel electrophoresis to view their protein profile. Results: The results showed that the cold extraction illustrates a maximum zone of inhibition against Bacillus subtillis (B. subtillis), Escherichia coli (E. coli) viz., (17.00 ± 2.91), (14.50 ± 1.71) mm, followed by hot extraction against, E. coli, Salmonella sp. as (11.66 ± 3.14), (10.60 ± 3.09) mm. In methanol extraction showed a highest zone of inhibition recorded against B. subtillis, E. coli as (18.86 ± 0.18), (18.00 ± 1.63) mm pursued by ethanol extraction showed utmost zone of inhibition recorded against Salmonella sp. at (20.40 ± 1.54) mm. The crude protein from flower showed a maximum inhibitory zone observed against Salmonella sp., E. coliviz., (16.55 ± 1.16), (14.30 ± 2.86) mm. The flower material can be taken as an alternative source of antibacterial agent against the human pathogens. Conclusions: The extracts of the H. rosa-sinensis are proved to have potential antibacterial activity, further studies are highly need for the drug development.

  17. Combination of Dragon Fruit, Hibiscus and Bitterleaf as Dye Sensitizer to Increase Efficiency of DSSC

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    Asful Hariyadi

    2017-06-01

    Full Text Available Dye-sensitized Solar Cell (DSSC from natural products was being prominently developed in recent years. The main advantage of DSSC was its fabrication which did not require high process technology, so the production cost was lower than solar cells from crystalline materials. There were important materials in Dye-sensitized Solar Cell technology, such as: Zinc Oxide as an electrode, electrolyte solution and plant pigments as dye sensitizer to absorb light energy and convert it into a source of electrical energy. In this study, the combination of multiple dye mixture had been investigated to achieve high external quantum efficiency as sensitizers. Furthermore, each sample would be tested to determine the characteristics of natural dye and to calculate the power flow and efficiency of the electricity generated by each variable of dye solution. There would be four variables in this study, which was the composition (% v/v of the three main sources of natural dyes; hibiscus, bitter leaf and dragon fruit, respectively first (50% : 25% :25%, second (25% : 50% : 25%, third (25% : 25% : 50% and fourth (33,33% : 33,33% : 33,33%. The result of the study showed that efficiency of variable 1 to 4 was 1.9076%, 2.8739%, 0.8566% and 0.8344%, respectively

  18. Hybrid Sex Pheromones of the Hibiscus Flower-bud Borer, Rehimena surusalis.

    Science.gov (United States)

    Honda, Hiroshi; Yamasaki, Ryokuhei; Sumiuchi, Yoko; Uehara, Takuya; Matsuyama, Shigeru; Ando, Tetsu; Naka, Hideshi

    2015-11-01

    The sex pheromone of the hibiscus flower borer Rehimena surusalis (Walker) (Lepidoptera: Crambidae) was analyzed by gas chromatography with electroantennographic detection (GC-EAD) and GC-mass spectrometry (GC/MS). Three EAD-active components were found in crude pheromone gland extracts of calling females. GC/MS and GC analyses using synthetic chemicals and derivatization of the extracts identified three components as (10E,12Z)-hexadeca-10,12-dienal (E10,Z12-16:Ald,), (10E,12E)-hexadeca-10,12-dienyl acetate (E10,Z12-16:OAc), and (3Z,6Z,9Z)-tricosa-3,6,9-triene (Z3,Z6,Z9-23:HC). In field tests, male moths were strongly attracted to a ternary blend of E10,Z12-16:Ald, E10,Z12-16:OAc, and Z3,Z6,Z9-23:HC at a ratio of 1:5:14, but single and binary blends showed only weak or no attraction.

  19. Genome analysis of Hibiscus syriacus provides insights of polyploidization and indeterminate flowering in woody plants

    Science.gov (United States)

    Kim, Yong-Min; Kim, Seungill; Koo, Namjin; Shin, Ah-Young; Yeom, Seon-In; Seo, Eunyoung; Park, Seong-Jin; Kang, Won-Hee; Kim, Myung-Shin; Park, Jieun; Jang, Insu; Kim, Pan-Gyu; Byeon, Iksu; Kim, Min-Seo; Choi, JinHyuk; Ko, Gunhwan; Hwang, JiHye; Yang, Tae-Jin; Choi, Sang-Bong; Lee, Je Min; Lim, Ki-Byung; Lee, Jungho; Choi, Ik-Young; Park, Beom-Seok; Kwon, Suk-Yoon; Choi, Doil

    2017-01-01

    Abstract Hibiscus syriacus (L.) (rose of Sharon) is one of the most widespread garden shrubs in the world. We report a draft of the H. syriacus genome comprised of a 1.75 Gb assembly that covers 92% of the genome with only 1.7% (33 Mb) gap sequences. Predicted gene modeling detected 87,603 genes, mostly supported by deep RNA sequencing data. To define gene family distribution among relatives of H. syriacus, orthologous gene sets containing 164,660 genes in 21,472 clusters were identified by OrthoMCL analysis of five plant species, including H. syriacus, Arabidopsis thaliana, Gossypium raimondii, Theobroma cacao and Amborella trichopoda. We inferred their evolutionary relationships based on divergence times among Malvaceae plant genes and found that gene families involved in flowering regulation and disease resistance were more highly divergent and expanded in H. syriacus than in its close relatives, G. raimondii (DD) and T. cacao. Clustered gene families and gene collinearity analysis revealed that two recent rounds of whole-genome duplication were followed by diploidization of the H. syriacus genome after speciation. Copy number variation and phylogenetic divergence indicates that WGDs and subsequent diploidization led to unequal duplication and deletion of flowering-related genes in H. syriacus and may affect its unique floral morphology. PMID:28011721

  20. Preliminary X-ray Data Analysis of Crystalline Hibiscus Chlorotic Ringspot Virus

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, A.; Speir, J; Yuan, Y; Johnson, J; Wong, S

    2009-01-01

    Hibiscus chlorotic ringspot virus (HCRSV) is a positive-sense monopartite single-stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family, which includes carnation mottle virus (CarMV). The HCRSV virion has a 30 nm diameter icosahedral capsid with T = 3 quasi-symmetry containing 180 copies of a 38 kDa coat protein (CP) and encapsidates a full-length 3.9 kb genomic RNA. Authentic virus was harvested from infected host kenaf leaves and was purified by saturated ammonium sulfate precipitation, sucrose density-gradient centrifugation and anion-exchange chromatography. Virus crystals were grown in multiple conditions; one of the crystals diffracted to 3Synchrotron .2 A resolution and allowed the collection of a partial data set. The crystal belonged to space group R32, with unit-cell parameters a = b = 336.4, c = 798.5 . Packing considerations and rotation-function analysis determined that there were three particles per unit cell, all of which have the same orientation and fixed positions, and resulted in tenfold noncrystallography symmetry for real-space averaging. The crystals used for the structure determination of southern bean mosaic virus (SBMV) have nearly identical characteristics. Together, these findings will greatly aid the high-resolution structure determination of HCRSV.

  1. Phenolic Content and Antioxidant Activity of Hibiscus cannabinus L. Seed Extracts after Sequential Solvent Extraction

    Directory of Open Access Journals (Sweden)

    Shahid Iqbal

    2012-10-01

    Full Text Available A sequential solvent extraction scheme was employed for the extraction of antioxidant compounds from kenaf (Hibiscus cannabinus L. seeds. Yield of extracts varied widely among the solvents and was the highest for hexane extract (16.6% based on dry weight basis, while water extract exhibited the highest total phenolic content (18.78 mg GAE/g extract, total flavonoid content (2.49 mg RE/g extract, and antioxidant activities (p < 0.05. DPPH and hydroxyl radical scavenging, β-carotene bleaching, metal chelating activity, ferric thiocyanate and thiobarbituric acid reactive substances assays were employed to comprehensively assess the antioxidant potential of different solvent extracts prepared sequentially. Besides water, methanolic extract also exhibited high retardation towards the formation of hydroperoxides and thiobarbituric acid reactive substances in the total antioxidant activity tests (p < 0.05. As conclusion, water and methanol extracts of kenaf seed may potentially serve as new sources of antioxidants for food and nutraceutical applications.

  2. Phytotreatment of soil contaminated with used lubricating oil using Hibiscus cannabinus.

    Science.gov (United States)

    Abioye, O P; Agamuthu, P; Abdul Aziz, A R

    2012-04-01

    Soil contamination by hydrocarbons, especially by used lubricating oil, is a growing problem in developing countries, which poses a serious threat to the environment. Phytoremediation of these contaminated soils offers environmental friendly and a cost effective method for their remediation. Hibiscus cannabinus was studied for the remediation of soil contaminated with 2.5 and 1% used lubricating oil and treated with organic wastes [banana skin (BS), brewery spent grain (BSG) and spent mushroom compost (SMC)] for a period of 90 days under natural conditions. Loss of 86.4 and 91.8% used lubricating oil was recorded in soil contaminated with 2.5 and 1% oil and treated with organic wastes respectively at the end of 90 days. However, 52.5 and 58.9% oil loss was recorded in unamended soil contaminated with 2.5 and 1% oil, respectively. The plant did not accumulate hydrocarbon from the soil but shows appreciable accumulation of Fe and Zn in the root and stem of H. cannabinus at the end of the experiment. The first order kinetic rate of uptake of Fe and Zn in H. cannabinus was higher in organic wastes amendment treatments compared to the unamended treatments, which are extremely low. The results of this study suggest that H. cannabinus has a high potential for remediation of hydrocarbon and heavy metal contaminated soil.

  3. Accumulation of Kaempferitrin and Expression of Phenyl-Propanoid Biosynthetic Genes in Kenaf (Hibiscus cannabinus

    Directory of Open Access Journals (Sweden)

    Shicheng Zhao

    2014-10-01

    Full Text Available Kenaf (Hibiscus cannabinus is cultivated worldwide for its fiber; however, the medicinal properties of this plant are currently attracting increasing attention. In this study, we investigated the expression levels of genes involved in the biosynthesis of kaempferitrin, a compound with many biological functions, in different kenaf organs. We found that phenylalanine ammonia lyase (HcPAL was more highly expressed in stems than in other organs. Expression levels of cinnamate 4-hydroxylase (HcC4H and 4-coumarate-CoA ligase (Hc4CL were highest in mature leaves, followed by stems and young leaves, and lowest in roots and mature flowers. The expression of chalcone synthase (HcCHS, chalcone isomerase (HcCHI, and flavone 3-hydroxylase (HcF3H was highest in young flowers, whereas that of flavone synthase (HcFLS was highest in leaves. An analysis of kaempferitrin accumulation in the different organs of kenaf revealed that the accumulation of this compound was considerably higher (>10-fold in leaves than in other organs. On the basis of a comparison of kaempferitrin contents with the expression levels of different genes in different organs, we speculate that HcFLS plays an important regulatory role in the kaempferitrin biosynthetic pathway in kenaf.

  4. Infarction of middle third posterior cortex of kidney: a complication of extended pyelolithotomy, intra-operative electrohydraulic lithotripsy and extraction of calyceal stones under vision using stone basket and flexible cystoscope in a spinal cord injury patient – a case report

    Science.gov (United States)

    2009-01-01

    Background Spinal cord injury produces multiple systemic and metabolic alterations. A decrease in micro vascular blood flow to liver, spleen and muscle has been described following spinal cord injury. Case presentation We present a 46-year-old male patient with C-4 complete tetraplegia, who developed a large stag horn calculus with branches in upper, middle and lower calyces of left kidney. This patient underwent Gil-Vernet extended pyelolithotomy and required intra-operative electrohydraulic lithotripsy and retrieval of stones from upper, middle and lower calyces using flexible cystoscope and stone basket. Computed tomography, performed eighteen days after surgery, showed multiple areas of non-enhancing cortex posteriorly and in the upper pole, suggestive of focal infarction. Magnetic resonance imaging of left kidney confirmed the presence of an area of infarction in middle third of posterior cortex, but there was no evidence of trauma to posterior division of renal artery. Therefore, we postulate that compression of renal parenchyma by Gil-Vernet retractors during surgery, and firm pressure that was applied over the middle of kidney for prolonged periods while several attempts were being made to retrieve fragments of calculi from renal calyces, led to ischaemia and subsequently, infarction of mid-third posterior cortex of left kidney. Conclusion This case illustrates importance of gentle handling of kidney during extended pyelolithotomy in order to prevent subtle renal trauma, which may be detected only by advanced imaging studies. Further, spinal cord physicians should take a pragmatic approach to management of stones located inside renal calyces. Both spinal cord injury patients and their physicians should remember that in our enthusiasm to achieve complete clearance of stones embedded deeply within renal calyces, we could produce irreversible injury to kidney, as indeed happened in this patient. Therefore, emphasis should be placed on prevention of struvite

  5. Bioclimatic thresholds, thermal constants and survival of mealybug, Phenacoccus solenopsis (hemiptera: pseudococcidae) in response to constant temperatures on hibiscus.

    Science.gov (United States)

    Sreedevi, Gudapati; Prasad, Yenumula Gerard; Prabhakar, Mathyam; Rao, Gubbala Ramachandra; Vennila, Sengottaiyan; Venkateswarlu, Bandi

    2013-01-01

    Temperature-driven development and survival rates of the mealybug, Phenacoccussolenopsis Tinsley (Hemiptera: Pseudococcidae) were examined at nine constant temperatures (15, 20, 25, 27, 30, 32, 35 and 40°C) on hibiscus (Hibiscusrosa -sinensis L.). Crawlers successfully completed development to adult stage between 15 and 35°C, although their survival was affected at low temperatures. Two linear and four nonlinear models were fitted to describe developmental rates of P. solenopsis as a function of temperature, and for estimating thermal constants and bioclimatic thresholds (lower, optimum and upper temperature thresholds for development: Tmin, Topt and Tmax, respectively). Estimated thresholds between the two linear models were statistically similar. Ikemoto and Takai's linear model permitted testing the equivalence of lower developmental thresholds for life stages of P. solenopsis reared on two hosts, hibiscus and cotton. Thermal constants required for completion of cumulative development of female and male nymphs and for the whole generation were significantly lower on hibiscus (222.2, 237.0, 308.6 degree-days, respectively) compared to cotton. Three nonlinear models performed better in describing the developmental rate for immature instars and cumulative life stages of female and male and for generation based on goodness-of-fit criteria. The simplified β type distribution function estimated Topt values closer to the observed maximum rates. Thermodynamic SSI model indicated no significant differences in the intrinsic optimum temperature estimates for different geographical populations of P. solenopsis. The estimated bioclimatic thresholds and the observed survival rates of P. solenopsis indicate the species to be high-temperature adaptive, and explained the field abundance of P. solenopsis on its host plants.

  6. Bioclimatic thresholds, thermal constants and survival of mealybug, Phenacoccus solenopsis (hemiptera: pseudococcidae in response to constant temperatures on hibiscus.

    Directory of Open Access Journals (Sweden)

    Gudapati Sreedevi

    Full Text Available Temperature-driven development and survival rates of the mealybug, Phenacoccussolenopsis Tinsley (Hemiptera: Pseudococcidae were examined at nine constant temperatures (15, 20, 25, 27, 30, 32, 35 and 40°C on hibiscus (Hibiscusrosa -sinensis L.. Crawlers successfully completed development to adult stage between 15 and 35°C, although their survival was affected at low temperatures. Two linear and four nonlinear models were fitted to describe developmental rates of P. solenopsis as a function of temperature, and for estimating thermal constants and bioclimatic thresholds (lower, optimum and upper temperature thresholds for development: Tmin, Topt and Tmax, respectively. Estimated thresholds between the two linear models were statistically similar. Ikemoto and Takai's linear model permitted testing the equivalence of lower developmental thresholds for life stages of P. solenopsis reared on two hosts, hibiscus and cotton. Thermal constants required for completion of cumulative development of female and male nymphs and for the whole generation were significantly lower on hibiscus (222.2, 237.0, 308.6 degree-days, respectively compared to cotton. Three nonlinear models performed better in describing the developmental rate for immature instars and cumulative life stages of female and male and for generation based on goodness-of-fit criteria. The simplified β type distribution function estimated Topt values closer to the observed maximum rates. Thermodynamic SSI model indicated no significant differences in the intrinsic optimum temperature estimates for different geographical populations of P. solenopsis. The estimated bioclimatic thresholds and the observed survival rates of P. solenopsis indicate the species to be high-temperature adaptive, and explained the field abundance of P. solenopsis on its host plants.

  7. Calidad seminal en ovinos pelibuey con inclusión de Hibiscus rosa-sinensis en la dieta

    OpenAIRE

    José Maza Gamboa; Luis Fernando Navarrete Sierra; Alfonso Aguiar Loría; Roberto Zamora Bustillos; Héctor Magaña Sevilla

    2015-01-01

    La alimentación de los ovinos en el trópico representa del 48 a 90 % de los costos de producción, además, el uso de granos hace dependientes a los ovinocultores del mercado internacional. Ganancias de peso moderadas se han obtenido con follaje de plantas arbustivas, sin embargo, algunas plantas como el Tulipán (Hibiscus rosa-sinensis) presentan compuestos que pueden actuar como fito-estrógenos, lo que puede comprometer el uso potencial en la alimentación de sementales ovinos pelibuey. Método:...

  8. Antisnake Venom Activity of Hibiscus aethiopicus L. against Echis ocellatus and Naja n. nigricollis

    Directory of Open Access Journals (Sweden)

    S. S. Hasson

    2010-01-01

    Full Text Available The objective of the study is to investigate whether the Hibiscus aethiopicus L. plant has neutralization activity against venoms of two clinically important snakes. The H. aethiopicus was dried and extracted with water. Different assays were performed to evaluate the plant's acute toxicity and its anti-snake venom activities. The results showed that H. aethiopicus extract alone had no effect on the viability of C2C12 muscle cells, but significantly (P<.05 protected muscle cells against the toxic effects of E. ocellatus venom at 55, 150, and 300 μg/mL. The maximum protective effect of the extract was exhibited at 75 μg/mL. The extract significantly (P<.001 inhibited the cytotoxic effects of E. ocellatus venom at 300 μg/mL. All rabbits (n=10 and guinea pigs (n=10 were alive after the two weeks of given the lethal dosage 16 g/Kg of the H. aethiopicus extract herbal solution. No abnormal behaviour was observed of both groups of animals. All guinea pigs (n=3 treated with venoms alone (5 mg/kg died. However, all guinea pigs (n=21 treated with venom (5 mg/kg and the extract (400 to 1000 mg/kg survived. Guinea pigs (n=3 treated with Naja n. nigricollis venom alone (2.5 mg/kg and guinea pigs (n=21 venom with the extract (400 to 1000 mg/kg died. The H. aethiopicus completely (100% blocked the haemorrhagic activity of E. ocellatus in the egg embryo at 3.3 mg/mL of extract. These findings suggest that H. aethiopicus may contain an endogenous inhibitor of venom-induced haemorrhage.

  9. Comparison of sex pheromone traps for monitoring pink hibiscus mealybug (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Vitullo, Justin; Wang, Shifa; Zhang, Aijun; Mannion, Catharine; Bergh, J Christopher

    2007-04-01

    The pink hibiscus mealybug, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae), is a highly polyphagous pest that invaded Florida in 2002 and has recently been reported from several locations in Louisiana. Although identification of its sex pheromone in 2004 improved monitoring capabilities tremendously, the effectiveness and efficiency of different pheromone trap designs for capturing males has not been evaluated. We deployed green Delta, Pherocon IlB, Pherocon V, Jackson, and Storgard Thinline traps in Homestead, FL, and compared the number of male M. hirsutus captured per trap, the number captured per unit of trapping surface area, the amount of extraneous material captured, and the time taken to count trapped mealybugs. Pheromone-baited traps with larger trapping surfaces (green Delta, Pherocon IIB, and Pherocon V) captured more males per trap than those with smaller surfaces (Jackson and Storgard Thinline), and fewest males were captured by Storgard Thinline traps. However, Jackson traps captured as many or more males per square centimeter of trapping surface as those with larger surfaces, and the time required to count males in Jackson traps was significantly less than in green Delta, Pherocon IIB, and Pherocon V traps. Although all trap designs accumulated some debris and nontarget insects, it was rated as light to moderate for all designs. Based on our measures of effectiveness and efficiency, the Jackson trap is most suitable for monitoring M. hirsutus populations. Additionally, unlike the other traps evaluated, which must be replaced entirely or inspected in the field and then redeployed, only the sticky liners of Jackson traps require replacement, enhancing the efficiency of trap servicing.

  10. Effects of extracts from Hibiscus macranthus and Basella alba mixture on testosterone production in vitro in adult rat testes slices

    Institute of Scientific and Technical Information of China (English)

    Paul F. Moundipa; Silvère Ngouela; Pierre Kamtchouing; Etienne Tsamo; Félicité M. Tchouanguep; Serge Carreau

    2006-01-01

    Aim: To find an in vitro system for the measurement of the androgenic effects of different extracts of Hibiscus macranthus (Malvaceae) and Basella alba (Basellaceae). Methods: The production of testosterone from testes slices incubated in two media, either Krebs-Henseleit buffer containing 0.5 % Bovine serum albumin (BSA) or Dubecco's Modified Eagle's medium-F12 Ham nutrient mixture (DME/Ham F12), under a mixture of 5 % CO2 in 95 % air was determined either in the presence or absence of cofactors and Hibiscus macranthus plus Basella alba (HMBA)extracts. Results: The testosterone production was increased in testes slices incubated in DME/Ham F12 medium in response to the cofactors (49 %) and aqueous extracts (34 %-60 % according to dilutions). Under the same atmospheric conditions, there was no positive response of the testes slices to either cofactor or HMBA extract stimulation in Krebs-Henseleit buffer containing 0.5 % BSA. In further investigations related to the effect of HMBA, the DME/Ham F12 medium was used. The results obtained from the in vitro test showed that the activity was present mainly in methylene chloride and methanol, since these extracts induced an increase in testosterone production by testes slices. Conclusion: The testes slice system is suitable to be used for further in vitro investigations of the isolation of androgenic bioactive components of plants.

  11. Formulation Development and Characterization of Hibiscus Rosa-Sinesis Dry Leaves Mucilage as Smart Polymer for Pharmaceutical Use

    Directory of Open Access Journals (Sweden)

    Prabakaran Lakshmanan

    2015-03-01

    Full Text Available Summary. The present investigation was to extract the Hibiscus Rosa Sinensis dry leaves mucilage and prove to be a smart polymer in pharmaceutical formulations. Firstly to formulate and optimize controlled-release floating tablets of Nizatidine (NF1-NF5 using HPMC K 100M and Hibiscus dry leaves mucilage (5-10% along with gas generating agent sodium bicarbonate by direct compression technique. Results revealed that increase the mucilage concentration decrease the release of drug from floating tablets. The NF1 formulation, NF2, NF3 and NF4, and NF5 showed fickian type (n - 0.36, anomalous/non-fickian type (n - 0.5-1.0 and super case II transport mechanism ("n" - 1.449 respectively and secondly to formulate Mesalamine core tablets using Hibiscus Rosa Sinensis dry leaves mucilage (5-20% and to be coated with Eudragit L 100 mixture by dip coating technique (MFI-MFIV to target the drug release in colon. Release study showed that the MFI and MFII showed first order release (“n” - 0.45, MFIII showed matrix model with the “n” value of 0.5 (fickian diffusion, and MFIV showed peppas model (n - <0.6 respectively. It was concluded that the mucilage plays well in both the acidic and alkaline environment of the GI tract, controlled drug release with floating and colon targeting to solve the issues of those particular targets.Industrial relevance. In certain drug like Nizatidine, the therapeutic efficacy is improved if the gastric residence time of the dosage form is increased at the absorption site and if the drug is highly soluble in aqueous environment, it is necessary to reduce/controls the drug release from the formulations. Hibiscus-Rosa Sinesis leaf mucilage exhibit excellent retarding effect on drug release from the floating tablets. Colon targeted tablets manufactured with conventional excipients, upon reaching the colon, the tablet bursts as it comes in contact with water causing dose dumping that can pose a significant risk to patients

  12. Mesoscale modelling of water vapour in the tropical UTLS: two case studies from the HIBISCUS campaign

    Directory of Open Access Journals (Sweden)

    V. Marécal

    2007-01-01

    Full Text Available In this study, we evaluate the ability of the BRAMS (Brazilian Regional Atmospheric Modeling System mesoscale model compared to ECMWF global analysis to simulate the observed vertical variations of water vapour in the tropical upper troposphere and lower stratosphere (UTLS. The observations are balloon-borne measurements of water vapour mixing ratio and temperature from micro-SDLA (Tunable Diode Laser Spectrometer instrument. Data from two balloon flights performed during the 2004 HIBISCUS field campaign are used to compare with the mesoscale simulations and to the ECMWF analysis. The observations exhibit fine scale vertical structures of water vapour of a few hundred meters height. The ECMWF vertical resolution (~1 km is too coarse to capture these vertical structures in the UTLS. With a vertical resolution similar to ECMWF, the mesoscale model performs better than ECMWF analysis for water vapour in the upper troposphere and similarly or slightly worse for temperature. The BRAMS model with 250 m vertical resolution is able to capture more of the observed fine scale vertical variations of water vapour compared to runs with a coarser vertical resolution. This is mainly related to: (i the enhanced vertical resolution in the UTLS and (ii to the more detailed microphysical parameterization providing ice supersaturations as in the observations. In near saturated or supersaturated layers, the mesoscale model predicted relative humidity with respect to ice saturation is close to observations provided that the temperature profile is realistic. For temperature, the ECMWF analysis gives good results partly attributed to data assimilation. The analysis of the mesoscale model results showed that the vertical variations of the water vapour profile depends on the dynamics in unsaturated layer while the microphysical processes play a major role in saturated/supersaturated layers. In the lower stratosphere, the ECMWF model and the BRAMS model give very similar

  13. Effects of ultraviolet radiation on Hibiscus rosa-sinensis, Beta vulgaris and Helianthus annuus

    Energy Technology Data Exchange (ETDEWEB)

    Panagopoulos, I.

    1992-01-01

    It is believed that increased levels of ultraviolet B-radiation (UV-B;280-320 nm) will result in serious threat to plant. In the present study the effects of UV (particularly UV-B) were studied on chlorophyll fluorescence, ultraweak luminescence (UL) and plant growth. Parameters related to light emission were determined, and the effects of UV-B on hypocotyl elongation and levels of free IAA were examined. The plants were grown in greenhouse or in growth chambers and exposed to short or long term UV-B simulating different levels of ozone depletion. Short exposure of Hibiscus leaves to UV resulted in a gradual increase in both UL and peroxidase activity followed by a decline after 72 h and a decrease in variable chlorophyll fluorescence. The action of UV-B on sugar beet plants depended on light quality and irradiance and infection by Cercospora beticola Sacc. The interaction between UV-B and the disease resulted in a large reduction of dry weight and enhanced UL. The lowest Chl a and growth was found in plants grown under low irradiance and exposed to UV-B supplemented with UV-A (320-400 nm). UVB also inhibited photosystem II, increased UL and peroxidase activity. Under relatively high PAR, UV-B increased dry weight of laminae and UL but no effect on Chl content. Sugar beet plants grown with light depleted in the 320-400 nm region of the spectrum and exposed to UV-B died. Low levels of UV-B did neither affected hypocotyl elongation nor amounts of free IAA in sunflower plants grown under low (LL; 143 [mu]mol m[sup -2]s[sup -1]) or high PAR (HL; 800 [mu]mol m[sup -2]s[sup -1]). Three times more daily UV-B increased the amount of free IAA, but inhibited hypocotyl elongation. Higher F[sub v]/F[sub max] and F690/F735, Chl a and carotenoids were found in plants exposed to low UV-B. Indeed, UV-B can be harmful but may also have enhancing effects on plants. (au) (114 refs.).

  14. Methanol leaves extract Hibiscus micranthus Linn exhibited antibacterial and wound healing activities.

    Science.gov (United States)

    Begashaw, Berhan; Mishra, Bharat; Tsegaw, Asegedech; Shewamene, Zewdneh

    2017-06-26

    Infectious diseases are the most common causes of morbidity and mortality in developing countries. Wound and wound infections are also major health problem. Nowadays, medicinal plants play a major role in treatment of infectious diseases and wound healing and they are easily available and more affordable as compared to synthetic compounds. The aim of this study is therefore, to investigate the antibacterial and wound healing activities of 80% methanol extract of Hibiscus micranthus leaves using disc diffusion methods and rat excision model respectively. In vitro antibacterial screening was carried out against S. aureus, S.pneumoniae, S. pyogenes, E. coli, P. aeruginosa, K. pneumoniae and P. mirabilis bacterial strains using disc-well diffusion assay. Would healing activity was done in rats divided into four groups each consisting of six animals. Group I was served as a negative control (ointment base), Group II served as a positive control Nitrofurazone (NFZ 0.2% ointment), Groups III and IV was treated 5 and 10% extracts respectively. The acute oral toxicity test and skin sensitivity test were also performed before conducting the actual study. The extract was analyzed for secondary metabolites using standard methods. Preliminary phytochemical screening have revealed the presence of alkaloids, flavonoids, saponins, tannins, steroids, phenols, diterpines, anthraquinones and the absence of glycosides, terpinoides and triterpines. Based on acute oral toxicity test the extract was found to be safe up to a dose of 2 g/kg. In addition, acute dermal toxicity test indicated no sign of skin irritation. The leaves extract exhibited varying degrees of sensitivity with zones of inhibition ranging from 14.00 ± 0.333 (S.pyogenes) to 22.67 ± 1.202 mm (S.aureus). It was found that S. aureus and S. pneumonia (p wound healing study, the 5 and 10% w/w extract exhibited significant wound contraction rate of 99.30% and 99.13% as compared to NFZ ointment and simple ointment

  15. A DISTRIBUTIONAL AND CYTOLOGICAL SURVEY OF THE PRESENTLY RECOGNIZED TAXA OF HIBISCUS SECTION FURCARIA (MALVACEAE Distribución y citología de los taxones reconocidos actualmente de Hibiscus sección Furcaria (Malvaceae

    Directory of Open Access Journals (Sweden)

    F. Douglas Wilson

    2010-08-01

    Full Text Available

    Hibiscus section Furcaria is a natural group of plants that presently includes 109 recognized
    taxa. Taxa are found in subsaharan Africa, India, southeastern Asia, Malesia, Australia, islands
    of the Pacific basin, the Caribbean, North, Central, and South America. The basic chromosome
    number is x = 18. In nature, ploidy levels range from diploid to decaploid. The taxa exhibit a
    remarkable amount of genome diversity. At least 13 genomes have been identified, some
    distributed widely and others with more restricted distributions. No modern taxonomic
    monograph of Hibiscus section Furcaria exists, but a number of regional studies have
    appeared that are essentially global in extent. Also, a number of studies of chromosome
    numbers and genome relationships have been published. The present paper includes a
    census of all the presently accepted taxa, the geographical distribution of each taxon, and
    chromosome numbers and genome designations of the 49 taxa for which the information is
    available. Important mechanisms of speciation include genome divergence at the diploid level,
    followed by hybridization and allopolyploidy, significant species radiation at the tetraploid and
    hexaploid levels, and the development of even higher levels of allopolyploids
    Hibiscus sección Furcaria es un grupo natural de plantas que actualmente incluye 109 taxones
    reconocidos. Los taxones habitan en África al sur del Sahara, India, sudeste de Asia, Malasia,
    Australia, Islas del Pacífico, el Caribe, América del Norte, Central y Sudamérica. El número
    básico de cromosomas es x = 18. En la naturaleza, los niveles de ploidía varían de diploide a
    decaploide. Los taxones exhiben una remarcable diversidad genómica. Han sido identificados
    al menos 13 genomas, algunos distribuidos

  16. EFEITOS DE PRODUTOS QUÍMICOS NO CONTROLE DE Meloidogyne sp SOBRE QUIABEIRO (Hibiscus sculentum L. EFFECTS OF CHEMICAL CONTROL OF Meloidogyne sp. IN Hibiscus sculentum L. IN OKRA

    Directory of Open Access Journals (Sweden)

    Lincoln Fonseca Zica

    2007-09-01

    Full Text Available

    Testou-se o poder nematicida de Shell-DD, Vapan, Nemagon granulado, brometo de metila e Neantina solúvel em solos com infestacão natural de Meloidogyne sp., usando-se o quiabeiro (Hibiscus escullentum L. como referência para medir a intensidade de infestação após o tratamento. O Shell-DD (40 ml/m² exerceu notável efeito nematicida, mas o Vapan (60 ml/m², o Nemagon granulado (40 g/m² e a Neantina solúvel (solução a 0,3% - 10 l/m² deram resultados estatisticamente iguais à testemunha. As parcelas tratadas com Brometo de metila (40 ml/m² foram perdidas em consequência do efeito fitotóxico do produto sobre as sementes e plântulas do quiabeiro.

    The nematicide power of Shell-DD, vapan, granulated nemagon, methyl bromide and soluble neantina, were tested by applying them to soil affected by Meloidogyne sp. The okra plant (Hibiscus esculentum L. was used as the hosting agent, to measure the amount of worms af-ter treatment. The results showed that Shell-DD (40 ml/m² controlled the worms well, but that vapan, granulated nemagon and the soluble neantina were statistically equal to the control plot. The plots treated with methyl bromide were not included in the results because of the toxic effect of the product on the plants.

  17. [Characteristics of Cannabis sativa L.: seed morphology, germination and growth characteristics, and distinction from Hibiscus cannabinus L].

    Science.gov (United States)

    Yoshimatsu, Kayo; Kitazawa, Takashi; Kawano, Noriaki; Iida, Osamu; Kawahara, Nobuo

    2010-02-01

    Illegal cannabis (Cannabis sativa L.) cultivation is still a social problem worldwide. Fifty inquiries on cannabis that Research Center for Medicinal Plant Resources (Tsukuba Division) received between January 1, 2000 and March 31, 2009 were itemized in to 8 categories; 1: seed identification, 2: plant identification, 3: indoor cultivation, 4: outdoor cultivation, 5: germination and growth characteristics, 6: expected amount of cannabis products derived from illegal cannabis plant, 7: non-narcotic cannabis and 8: usage of medicinal cannabis. Top three inquiries were 1: seed identification (16 cases), 3: indoor cultivation (10 cases) and 4: outdoor cultivation (6 cases). Characteristics of cannabis, namely seed morphology, germination and growth characteristics, and distinction from kenaf (Hibiscus cannabinus L.) that is frequently misjudged as cannabis, were studied to contribute for prevention of illegal cannabis cultivation.

  18. Antimicrobial activity of apple, hibiscus, olive, and hydrogen peroxide formulations against Salmonella enterica on organic leafy greens.

    Science.gov (United States)

    Moore, Katherine L; Patel, Jitendra; Jaroni, Divya; Friedman, Mendel; Ravishankar, Sadhana

    2011-10-01

    Salmonella enterica is one of the most common bacterial pathogens implicated in foodborne outbreaks involving fresh produce in the last decade. In an effort to discover natural antimicrobials for use on fresh produce, the objective of the present study was to evaluate the effectiveness of different antimicrobial plant extract-concentrate formulations on four types of organic leafy greens inoculated with S. enterica serovar Newport. The leafy greens tested included organic romaine and iceberg lettuce, and organic adult and baby spinach. Each leaf sample was washed, dip inoculated with Salmonella Newport (10(6) CFU/ml), and dried. Apple and olive extract formulations were prepared at 1, 3, and 5% concentrations, and hibiscus concentrates were prepared at 10, 20, and 30%. Inoculated leaves were immersed in the treatment solution for 2 min and individually incubated at 4°C. After incubation, samples were taken on days 0, 1, and 3 for enumeration of survivors. Our results showed that the antimicrobial activity was both concentration and time dependent. Olive extract exhibited the greatest antimicrobial activity, resulting in 2- to 3-log CFU/g reductions for each concentration and type of leafy green by day 3. Apple extract showed 1- to 2-log CFU/g reductions by day 3 on various leafy greens. Hibiscus concentrate showed an overall reduction of 1 log CFU/g for all leafy greens. The maximum reduction by hydrogen peroxide (3%) was about 1 log CFU/g. The antimicrobial activity was also tested on the background microflora of organic leafy greens, and reductions ranged from 0 to 2.8 log. This study demonstrates the potential of natural plant extract formulations to inactivate Salmonella Newport on organic leafy greens.

  19. Attachment of 13 Types of Foodborne Bacteria to Jalapeño and Serrano Peppers and Antibacterial Effect of Roselle Calyx Extracts, Sodium Hypochlorite, Colloidal Silver, and Acetic Acid against These Foodborne Bacteria on Peppers.

    Science.gov (United States)

    Rangel-Vargas, Esmeralda; Gómez-Aldapa, Carlos A; Falfan-Cortes, Reyna N; Rodríguez-Marín, María L; Godínez-Oviedo, Angélica; Acevedo-Sandoval, Otilio A; Castro-Rosas, Javier

    2017-03-01

    Chili peppers are a very important crop in Mexico. However, these peppers have been associated with Salmonella infection outbreaks in the United States, and Salmonella and diarrheagenic Escherichia coli pathotypes have been isolated from jalapeño and serrano peppers in Mexico. To decrease microbial contamination of fruits and vegetables, chemical agents are commonly used; however, chemical agents used to eliminate pathogenic bacteria on vegetables have a limited antimicrobial effect. Roselle ( Hibiscus sabdariffa ) calyces have been reported to have an antimicrobial effect on pathogenic bacteria. In the present study, the antibacterial effect of four roselle calyx extracts (water, methanol, acetone, and ethyl acetate), sodium hypochlorite, colloidal silver, and acetic acid against foodborne bacteria was evaluated on contaminated jalapeño and serrano peppers. The 13 types of foodborne bacteria evaluated were Listeria monocytogenes , Shigella flexneri , Salmonella Typhimurium, Salmonella Typhi, Salmonella Montevideo, Staphylococcus aureus , E. coli O157:H7, five E. coli pathotypes (Shiga toxin producing, enteropathogenic, enterotoxigenic, enteroinvasive, and enteroaggregative), and Vibrio cholerae O1. All 13 types attached to both pepper types, with no significant differences in attachment between jalapeño and serrano peppers. Roselle calyx extract treatment resulted in a greater reduction in levels of all foodborne bacteria than did treatment with sodium hypochlorite, colloidal silver, and acetic acid on both pepper types. Roselle calyx extracts may be a useful for disinfection of chili peppers in the field, processing plants, restaurants, and homes.

  20. Antimicrobial Effect of Jasminum grandiflorum L. and Hibiscus rosa-sinensis L. Extracts Against Pathogenic Oral Microorganisms--An In Vitro Comparative Study.

    Science.gov (United States)

    Nagarajappa, Ramesh; Batra, Mehak; Sharda, Archana J; Asawa, Kailash; Sanadhya, Sudhanshu; Daryani, Hemasha; Ramesh, Gayathri

    2015-01-01

    To assess and compare the antimicrobial potential and determine the minimum inhibitory concentration (MIC) of Jasminum grandiflorum and Hibiscus rosa-sinensis extracts as potential anti-pathogenic agents in dental caries. Aqueous and ethanol (cold and hot) extracts prepared from leaves of Jasminum grandiflorum and Hibiscus rosa-sinensis were screened for in vitro antimicrobial activity against Streptococcus mutans and Lactobacillus acidophilus using the agar well diffusion method. The lowest concentration of every extract considered as the minimum inhibitory concentration (MIC) was determined for both test organisms. Statistical analysis was performed with one-way analysis of variance (ANOVA). At lower concentrations, hot ethanol Jasminum grandiflorum (10 μg/ml) and Hibiscus rosa-sinensis (25 μg/ml) extracts were found to have statistically significant (P≤0.05) antimicrobial activity against S. mutans and L. acidophilus with MIC values of 6.25 μg/ml and 25 μg/ml, respectively. A proportional increase in their antimicrobial activity (zone of inhibition) was observed. Both extracts were found to be antimicrobially active and contain compounds with therapeutic potential. Nevertheless, clinical trials on the effect of these plants are essential before advocating large-scale therapy.

  1. Candidatus Phytoplasma brasiliense associado ao superbrotamento do hibisco (Hibiscus rosa-sinensis L. no Estado de São Paulo Candidatus Phytoplasma brasiliense associated with hibiscus witches' broom in the State of São Paulo-Brazil

    Directory of Open Access Journals (Sweden)

    Eliane Gonçalves da Silva

    2009-09-01

    Full Text Available Plantas de hibisco com superbrotamento e definhamento seguido de morte têm sido observadas nos municípios de São Paulo, Campinas e Piracicaba. Como os sintomas são sugestivos daqueles induzidos por fitoplasmas, o presente trabalho buscou identificar o possível fitoplasma associado com a doença. Assim, 14 plantas sintomáticas de hibisco foram coletadas em Piracicaba (SP e submetidas ao PCR duplo com os primers P1/Tint-R16F2n/R2 e ao exame em microscópio eletrônico de transmissão. A identificação foi realizada por análise de RFLP com as enzimas de restrição BfaI, DraI, HaeIII, HhaI, HpaII, MboI, MseI, RsaI e TaqI. Testes de transmissão foram conduzidos com enxertia de ramos e uso de Cuscuta subinclusa. Os resultados de nested-PCR revelaram a presença consistente de fitoplasmas em todas as plantas sintomáticas e foram confirmados pela observação de corpúsculos pleomórficos no floema, através da microscopia eletrônica. A análise de RFLP mostrou que o fitoplasma encontrado em hibisco pertence ao grupo 16SrXV, o mesmo grupo do Candidatus Phytoplasma brasiliense. O fitoplasma foi transmitido de planta doente para sadia, tanto pela enxertia como pela C. subinclusa, demonstrando ser o agente do superbrotamento do hibisco.Ornamental hibiscus have been affected by shoot proliferation and decline followed by death in several cities in São Paulo State, especially São Paulo, Campinas and Piracicaba. As the symptoms are suggestives of those induced by phytoplasmas, the present work aimed to identify the possible phytoplasma associated with the disease. Fourteen symptomatic hibiscus were sampled in Piracicaba, submitted to nested-PCR with the primers P1/Tint-R16F2n/R2 and processed by transmission electron microscopy. The identification was made by RFLP analyses with the restriction enzymes BfaI, DraI, HaeIII, HhaI, HpaII, MboI, MseI, RsaI, and TaqI. Transmission assays were performed by grafting and Cuscuta subinclusa. The presence

  2. A survey on Hibiscus rosa-sinensis, Alcea rosea L. and Malva neglecta Wallr as antibacterial agents

    Institute of Scientific and Technical Information of China (English)

    Seyyed Mansour Seyyednejad; Haniyeh Koochak; Esmaeil Darabpour; Hossein Motamedi

    2010-01-01

    Objective:To guide for selection of plants with antibacterial activity for further phytochemical works on the isolation and identification of the active compounds. Methods: Ethanolic extracts of 3 species from Malvaceae family were evaluated by agar disc diffusion method for antibacterial activity against some gram-positive and gram-negative bacteria (Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Bacillus cereus, Bacillus anthracis, Escherichia coli and Streptococcus pyogenes). The extracts were obtained from aerial parts of Hibiscus rosa (H. rosa)-sinensis (leaf and flower), Alcea rosea (A. rosea) L. (leaf and flower) and Malva neglecta (M. neglecta) Wallr (flower). Results:These extracts had inhibitory effects at different concentrations (0.05, 0.10, 0.20 and 0.40 g/mL) against above mentioned bacteria. Escherichia coli was the most resistant strain. The highest inhibitory zone was showed by ethanolic extract of M. neglecta against Staphylococcus epidermidis (22 mm) and followed by ethanolic extract from flower of H. rosa against Staphylococcus epidermidis and Staphylococcus aureus (20 mm). The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values against Staphylococcus epidermidis were equal (MIC=MBC=5 mg/mL for M. neglecta extract and for H. rosa extract MIC=MBC=20 mg/mL). Conclusions:These findings suggest that these native plants have good antibacterial properties that can be used for infection control and treatment and could also be as new source for antibiotics discovery and infection treatment.

  3. Morus alba o Hibiscus rosa-sinensis como sustituto parcial de soya en dietas integrales para conejos

    Directory of Open Access Journals (Sweden)

    P. E. Lara

    2012-01-01

    Full Text Available Los conejos pueden aprovechar los nutrientes contenidos en los forrajes y subproductos agrícolas eficientemente gracias a la fermentación cecal y a la cecotrofía. Los objetivos del presente trabajo fueron: evaluar la tasa de crecimiento y el rendimiento de la canal en conejos alimentados con minibloques de harina de morera (Morus alba o tulipán (Hibiscus rosa-sinensis en sustitución parcial de pasta de soya; y determinar el consumo y digestibilidad de la dieta. Para esto, se utilizaron 18 conejos de 30 días de edad, distribuidos al azar en tres dietas experimentales (n=6, ac (alimento convencional, mbm (minibloque con 30% de morera y, mbt (minibloque con 27% de tulipán, durante nueve semanas. La ganancia diaria y el rendimiento en canal fue mejor (p0.10 en los tres grupos. Es factible sustituir parcialmente la proteína de la soya por harina de hojas de morera o tulipán, aunque presentan una menor respuesta productiva con respecto a la alimentación convencional.

  4. Studies on chemical constituents of Hibiscus mutabilis%木芙蓉叶化学成分研究

    Institute of Scientific and Technical Information of China (English)

    姚莉韵; 陆阳; 陈泽乃

    2003-01-01

    目的研究木芙蓉叶Hibiscus mutabilis的化学成分.方法采用硅胶柱、C18反相柱、聚酰胺柱色谱分离纯化,通过理化常数测定和光谱分析鉴定化合物的结构.结果从木芙蓉叶中分离得到10个化合物.根据波谱分析和理化数据,鉴定出其中9个化合物分别为:二十四烷酸(tetracosanoic acid,I)、β-谷甾醇(β-sitoster01,Ⅱ)、胡萝卜苷(daucosterol,Ⅲ)、水杨酸(salicylic acid,Ⅳ)、大黄素(emodin,Ⅴ)、芸香苷(rutin,Ⅵ)、山奈酚-3-O-β-芸香糖苷(kaempferol-3-O-β-rutinoside,Ⅶ)、山奈酚-3-O-β刺槐双糖苷(kaempferol-3-O-β-robinobinoside,Ⅷ)及山奈酚-3-O-β-D-(6-E-对羟基桂皮酰基)-葡萄糖苷[kaempferol-3-O-β-D-(6-E-p-hydroxycinnamoyl)-glucopyranoside,Ⅸ].结论除Ⅱ和Ⅵ外,其他化合物均为首次从该植物中分离得到.

  5. Effect of salt stress in the regulation of anthocyanins and color of hibiscus flowers by digital image analysis.

    Science.gov (United States)

    Trivellini, Alice; Gordillo, Belén; Rodríguez-Pulido, Francisco J; Borghesi, Eva; Ferrante, Antonio; Vernieri, Paolo; Quijada-Morín, Natalia; González-Miret, M Lourdes; Heredia, Francisco J

    2014-07-23

    The effect of salt stress (200 mM NaCl for 28 days) on physiological characteristics of Hibiscus rosa-sinensis, such as abscisic acid (ABA) content, electrolyte leakage, and photochemical efficiency in leaves, and its influence on biomass production, anthocyanin composition, and color expression of flowers were evaluated. Salinity significantly increased electrolyte leakage and ABA content in leaves and reduced the flower fresh weight. Chlorophyll fluorescence parameters were lower in salt stress condition, compared to control. Moreover, salt stress negatively affected the content of anthocyanins (mainly cyanidin-3-sophoroside), which resulted in a visually perceptible loss of color. The detailed anthocyanin composition monitored by HPLC-DAD-MS and the color variations by digital image analysis due to salt stress showed that the effect was more noticeable at the basal portion of petals. A forward stepwise multiple regression was performed for predicting the content of anthocyanins from appearance characteristics obtained by image analysis, reaching R-square values up to 0.90.

  6. Assessment of Pb uptake, translocation and immobilization in kenaf (Hibiscus cannabinus L.) for phytoremediation of sand tailings

    Institute of Scientific and Technical Information of China (English)

    HO Wai Mun; ANG Lai Hoe; LEE Don Koo

    2008-01-01

    The potential of kenaf (Hibiscus cannabinus L.) for phytoremediation of lead (Pb) on sand tailings was investigated. A pot experiment employing factorial design with two main effects of fertilizer and lead was conducted in a nursery using sand tailings from an ex-tin mine as the growing medium. Results showed that Pb was found in the root, stem, and seed capsule of kenaf but not in the leaf.Application of organic fertilizer promoted greater biomass yield as well as higher accumulation capacity of Pb. In Pb-spiked treatments,roots accumulated more than 85% of total plant Pb which implies that kenaf root can be an important sink for bioavailable Pb. Scanning transmission electron microscope (STEM) X-ray microanalysis confirmed that electron-dense deposits located along cell walls of kenaf roots were Pb precipitates. The ability of kenaf to tolerate Pb and avoid phytotoxicity could be attributed to the immobilization of Pb in the roots and hence the restriction of upward movement (translocation factor< 1). With the application of fertilizer, kenaf was also found to have higher biomass and subsequently higher bioaccumulation capacity, indicating its suitability for phytoremediation of Pb-contaminated site.

  7. Potential of kenaf (Hibiscus cannabinus L.) and corn (Zea mays L.) for phytoremediation of dredging sludge contaminated by trace metals.

    Science.gov (United States)

    Arbaoui, Sarra; Evlard, Aricia; Mhamdi, Mohamed El Wafi; Campanella, Bruno; Paul, Roger; Bettaieb, Taoufik

    2013-07-01

    The potential of kenaf (Hibiscus cannabinus L.) and corn (Zea mays L.) for accumulation of cadmium and zinc was investigated. Plants have been grown in lysimetres containing dredging sludge, a substratum naturally rich in trace metals. Biomass production was determined. Sludge and water percolating from lysimeters were analyzed by atomic absorption spectrometry. No visible symptoms of toxicity were observed during the three- month culture. Kenaf and corn tolerate trace metals content in sludge. Results showed that Zn and Cd were found in corn and kenaf shoots at different levels, 2.49 mg/kg of Cd and 82.5 mg/kg of Zn in kenaf shoots and 2.1 mg/kg of Cd and 10.19 mg/kg in corn shoots. Quantities of extracted trace metals showed that decontamination of Zn and Cd polluted substrates is possible by corn and kenaf crops. Tolerance and bioaccumulation factors indicated that both species could be used in phytoremediation.

  8. Free radical scavenging activity of three different flowers-Hibiscus rosa-sinensis, Quisqualis indica and Senna surattensis

    Institute of Scientific and Technical Information of China (English)

    Hazem Mohamed Mahmoud Hassan

    2016-01-01

    Objective: To evaluate three flowers of Hibiscus rosa-sinensis (H. rosa-sinensis), Quisqualis indica (Q. indica) and Senna surattensis (S. surattensis) for their antioxidant activity by different methods in addition to total phenolic, flavonoid and pigment contents. Methods: Antioxidant activity of water, ethanol and absolute ethanol extracts of three flowers; H. rosa-sinensis, Q. indica and S. surattensis was evaluated. The antioxidant activity was assessed by 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity, ferrous chelating activity, reducing power, nitric oxide scavenging activity, hydroxyl radical scavenging activity as well as total antioxidant capacity. Total flavonoids, total phenols and total pigments including chlorophylls and carotenoids were measured for the three flowers. Results: The results showed that the highest total antioxidant capacity at concentration of 500 mg/L was found in S. surattensis as 0.479 ± 0.001. Scavenging activity of H. rosa-sinensis, Q. indica and S. surattensis flower extracts against 1,1-diphenyl-2-picrylhydrazyl radical showed the highest activity of (90.20 ± 0.29)% with 500 mg/L. Phytochemical screening of the three flowers extracts were carried out for alkaloids, flavonoids, saponins, tannins, steroids, glycosides, terpenoids, amino acid and mucilages. H. rosa-sinensis showed the total phenolic in water extract of (235.77 ± 14.31) mg/100 g, the other two flowers Q. indica and S. surattensis had the total phenolic in ethanol ex-tracts of (937.70 ± 25.06) and (850.30 ± 13.81) mg/100 g, respectively. On the other hand total flavonoids were identified in absolute ethanol extracts in the three flowers [(32.83 ± 1.34), (49.24 ± 4.87) and (2.79 ± 0.23) mg/100 g, respectively]. Conclusions: The extracts in the constituents of the three flowers could be used as ad-ditives as supplement fractions in foods.

  9. Antagonistic Effects of Fertilizer on Photochemical Efficiency of Hibiscus cannabinus L. (Kenaf Planted on Beach Ridges Interspersed with Swales Soil

    Directory of Open Access Journals (Sweden)

    Mohd-Hazimy Yusoff

    2011-01-01

    Full Text Available Problem statement: Hibiscus cannabinus L. or Kenaf is a highly productive, warmseasonal C3 annual crop and is one of the potential candidates to substitute kenaf fiber as raw product for pulp and paper production. It survives well on less fertile soils including those of Beach Ridges Interspersed with Swales (BRIS soil. Approach: The objective of this study was to determine the effect of fertilizer on photochemical efficiency of H. cannabinus L. planted on BRIS soil using chlorophyll fluorescence technique. NPK with the ratio of 12:12:36 + 2MgO + TE (Trace-elements are mineral substances that act as an essential nutrients at a very low concentration and the micronutrient of the trace elements compositions are Boron, Copper, Iron, Manganese, Molybdenum and Zinc were used for fertilizer treatment. Three levels of fertilizer treatments were applied in three plots; high (1960 kg plot-1, medium (1260 kg plot-1 and low (700 kg plot-1 respectively each plot comprising 106, 000 plants and were planted in 20 lines. Photochemical efficiency in terms of Fv/Fm ratio was determined under water deficit condition, fertilizer toxicity and interaction of both factors. Results: Contrasting trends for photochemical parameters were observed between different fertilizer levels where antagonistic effects were found between the three fertilizer treatments. The mean values ranged for minimal fluorescence (Fo were from 256.27-273.06, maximal fluorescence (Fm were from 970-1110.5, variable fluorescence (Fv were from 705-854.23 and the ratio of Fv/Fm (photochemical efficiency were from 0.72-0.77, respectively. Hitherto, for stress level, percentage for the low fertilizer level was 23.5% as compared to medium with 26.8 and 27.6% for high fertilizer level. Conclusion: The present study revealed that an appropriate amount of fertilizer is required to maximize the yield production cost effectively.

  10. Indigenous perception and characterization of Yanyanku and Ikpiru: two functional additives for the fermentation of African locust bean.

    Science.gov (United States)

    Agbobatinkpo, Pélagie B; Azokpota, Paulin; Akissoe, Noël; Kayodé, Polycarpe; Da Gbadji, Rachelle; Hounhouigan, D Joseph

    2011-01-01

    Indigenous perception, processing methods, and physicochemical and microbiological characteristics of Yanyanku and Ikpiru, two additives used to produce fermented African locust bean condiments, Sonru and Iru, were evaluated. According to producers, these additives accelerate the fermentation and soften the texture of the condiments. Yanyanku is produced by spontaneous fermentation with either Hibiscus sabdariffa or Gossypium hirsutum or Adansonia digitata seeds, whereas only Hibiscus sabdariffa seeds are used for Ikpiru. Both additives, with pH values ranging between 6.2 and 10 and Bacillus spores varying between 5.5 and 8.9 Log(10) (CFU/g), could be considered as softening additives or enrichment inocula to produce condiments.

  11. Research on Salt-tolerance of Perennial Hibiscus moscheutos%多年生宿根花卉芙蓉葵耐盐性研究

    Institute of Scientific and Technical Information of China (English)

    王文成; 孙昌禹; 孙宇; 郭艳超; 薛志忠; 李克晔; 封晓辉

    2013-01-01

    为了给芙蓉葵在滨海盐渍地播种育苗和重盐碱地园林绿化应用提供科学依据,分别以芙蓉葵种子、幼苗和1 a生越冬根为试材,在人工模拟盐胁迫强度条件下,研究了不同浓度 NaCl溶液处理对芙蓉葵种子发芽期和苗期耐盐性,以及越冬根萌生苗耐盐性的影响。结果表明:0.2%盐度胁迫会促进芙蓉葵种子萌发,随着NaCl浓度的增大,种子初始萌发时间延长,发芽率和萌发初期生长均受到抑制,且抑制程度均随盐胁迫浓度的增大而逐渐增强,盐度>0.6%后会显著抑制种子萌发,但每天给予12 h光照可以显著提高发芽率;盐胁迫会抑制芙蓉葵幼苗生长,且抑制作用随盐胁迫浓度的增大而逐渐增强,不影响其正常形态性状的最高盐胁迫浓度为0.6%,当盐胁迫浓度超过该阈值后,会使芙蓉葵幼苗的观赏价值明显降低;芙蓉葵越冬根发芽期的耐盐能力较强,盐度>1.2%后成活率显著下降。根据生理指标测定和幼苗外观形态观测结果,推断芙蓉葵种子萌发和幼苗生长的耐盐阈值是0.6%,越冬根正常萌发的耐盐阈值是1.2%。%In order to provide a scientific basis for Hibiscus moscheutos sowing and raising seedlings in the coastal saline soil and landscaping applications in heavy saline soil , taking Hibiscus moscheutos seeds, seedlings and overwintering roots as test materials, the effects of different concentrations of NaCl solution treatments on Hibiscus moscheutos seeds germination and seedlings salt tolerance , and overwintering roots sprouting salinity tolerance were studied in the artificial simulating salt stress conditions.The results showed that 0.2% salinity stress could promote Hibiscus moscheutos seed germination, with the increasing of NaCl concentration , the initial time of seed germination was extended, the germination rate and germination growth were inhibited and the degree of

  12. Green Synthesis Methods of CoFe2O4 and Ag-CoFe2O4 Nanoparticles Using Hibiscus Extracts and Their Antimicrobial Potential

    Directory of Open Access Journals (Sweden)

    Dana Gingasu

    2016-01-01

    Full Text Available The cobalt ferrite (CoFe2O4 and silver-cobalt ferrite (Ag-CoFe2O4 nanoparticles were obtained through self-combustion and wet ferritization methods using aqueous extracts of Hibiscus rosa-sinensis flower and leaf. X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, and magnetic measurements were used for the characterization of the obtained oxide powders. The antimicrobial activity of the cobalt ferrite and silver-cobalt ferrite nanoparticles against Gram-positive and Gram-negative bacteria, as well as fungal strains, was investigated by qualitative and quantitative assays. The most active proved to be the Ag-CoFe2O4 nanoparticles, particularly those obtained through self-combustion using hibiscus leaf extract, which exhibited very low minimal inhibitory concentration values (0.031–0.062 mg/mL against all tested microbial strains, suggesting their potential for the development of novel antimicrobial agents.

  13. Study on Extraction Technology of Total Flavonoids in Hibiscus Manihot%金花葵花总黄酮提取工艺研究

    Institute of Scientific and Technical Information of China (English)

    吴迪; 王刚

    2013-01-01

    目的:研究提取金花葵花总黄酮的最佳工艺条件.方法:采用乙醇回流提取的方法,并利用正交实验优化工艺条件.结果:乙醇回流最佳提取工艺为14倍量70%乙醇回流1次,120 min/次,金花葵花中总黄酮的含量为4.38%.结论:优化提取工艺简单、稳定、可行.%Objective: To study the optimal extraction technology of total flavonoids in Hibiscus manihot. Methods : To adopt the ethanol refluxing extraction method, the conditions of ethanol circumfluence extraction were optimized by orthogonal test. Results : The ethanol circunifluence extracting method was the best.The optimal extraction technology was as follows: solid-liquid ratio 1 : 14, 70% ethanol, refluxing and extracting for 120 min, 2 times. The total flavonoids in Hibiscus manihot was 4.38%. Conclusion: This optimized process is simple, stable and efficient.

  14. (Psidium guajava), hibiscus (Hibiscus-rosa sinensis)

    African Journals Online (AJOL)

    SAM

    2014-04-30

    Apr 30, 2014 ... Author(s) agree that this article remain permanently open access under the terms of the Creative Commons ... therefore is a microhabitat on the surface of plant's leaf ..... culture-independent assessment of bacteria in the apple.

  15. OKRA(HIBISCUS ESCULENTUS)AND FENUGREEK(TRIGONELLA FOENUM GRACEUM)MUCILAGE:CHARACTERIZATION AND APPLICATION AS FLOCCULANTS FOR TEXTILE EFFLUENT TAEATMENTS

    Institute of Scientific and Technical Information of China (English)

    Rajani Srinivasan; Anuradha Mishra

    2008-01-01

    The use of new food grade polysaccharides (mucilage) obtained from Hibiscus esculentus and Trigonella foenum graceum,commonly called Okra and Fenugreek,respectively,as flocculants was described.These polysaccharides were used for removal of solids (suspended solids (SS) and total dissolved solids (TDS)) and dyes from real textile effluents and aqueous solutions of different class of synthetic dyes.Influences of varying polysaccharide concentration,contact time and pH on removal of pollutant from the textile wastewater were investigated.Results showed that polysaccharides (mucilage) obtained from Okra and Fenugreek were capable of removing 90%-94% of SS,30%-44% of TDS and 30%-35% of dye using a very low concentration of polysaccharide.X-ray diffraction (XRD) patterns of solid waste material obtained before and after the treatment with polysaccharides were used as a supportive evidence to explain the mechanism of flocculation.

  16. ADSORPTION POTENTIALITES OF BIO-SORBENTS DERIVED FROM PROSOPIS CINERARIA AND HIBISCUS ROSA-SIN ENSIS IN THE REMOVAL OF METHYL ORANGE DYE FROM POLLUTED WATERS

    Directory of Open Access Journals (Sweden)

    Shaik Karimulla

    2013-02-01

    Full Text Available Leaves, stems and their ashes of Prosopis cineraria and Hibiscus rosa-sinensis have been explored for their surface sorption abilities towards Methyl Orange Dye using simulated waters. Various physicochemical parameters such as pH, time of equilibration and sorbent concentrations are optimized for evoking the sorption potentialities of the plant materials for the maximum extraction of the Methyl Orange Dye from waters. The surface sorption nature is found to pH sensitive and % of removal is maximum near pH:3.0% removal of the Dye is more with ashes than respective bio-materials. Co-anions, in fivefold excess, are found to beinterfering in the order: trivalent anion>divalent > monovalent while co-cations have shown relatively less interference on the extraction of the Dye at optimum conditions of extraction. The adoptability of the methodologies developed is tested with some real industrial effluents.

  17. Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae, an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China

    Directory of Open Access Journals (Sweden)

    Kaiyun Guan

    2011-09-01

    Full Text Available Hibiscus aridicola (Malvaceae is an endangered ornamental shrub endemic to the dry-hot valleys of Jinsha River in southwest China. Only four natural populations of H. aridicola exist in the wild according to our field investigation. It can be inferred that H. aridicola is facing a very high risk of extinction in the wild and an urgent conservation strategy is required. By using a modified biotin-streptavidin capture method, a total of 40 microsatellite markers were developed and characterized in H. aridicola for the first time. Polymorphisms were evaluated in 39 individuals from four natural populations. Fifteen of the markers showed polymorphisms with two to six alleles per locus; the observed heterozygosity ranged from 0.19 to 0.72. These microsatellite loci would be useful tools for population genetics studies on H. aridicola and other con-generic species which are important to the conservation and development of endangered species.

  18. Hibiscus chlorotic ringspot virus coat protein is essential for cell-to-cell and long-distance movement but not for viral RNA replication.

    Directory of Open Access Journals (Sweden)

    Shengniao Niu

    Full Text Available Hibiscus chlorotic ringspot virus (HCRSV is a member of the genus Carmovirus in the family Tombusviridae. In order to study its coat protein (CP functions on virus replication and movement in kenaf (Hibiscus cannabinus L., two HCRSV mutants, designated as p2590 (A to G in which the first start codon ATG was replaced with GTG and p2776 (C to G in which proline 63 was replaced with alanine, were constructed. In vitro transcripts of p2590 (A to G were able to replicate to a similar level as wild type without CP expression in kenaf protoplasts. However, its cell-to-cell movement was not detected in the inoculated kenaf cotyledons. Structurally the proline 63 in subunit C acts as a kink for β-annulus formation during virion assembly. Progeny of transcripts derived from p2776 (C to G was able to move from cell-to-cell in inoculated cotyledons but its long-distance movement was not detected. Virions were not observed in partially purified mutant virus samples isolated from 2776 (C to G inoculated cotyledons. Removal of the N-terminal 77 amino acids of HCRSV CP by trypsin digestion of purified wild type HCRSV virions resulted in only T = 1 empty virus-like particles. Taken together, HCRSV CP is dispensable for viral RNA replication but essential for cell-to-cell movement, and virion is required for the virus systemic movement. The proline 63 is crucial for HCRSV virion assembly in kenaf plants and the N-terminal 77 amino acids including the β-annulus domain is required in T = 3 assembly in vitro.

  19. Evaluation of balloon and satellite water vapour measurements in the Southern tropical and subtropical UTLS during the HIBISCUS campaign

    Directory of Open Access Journals (Sweden)

    N. Montoux

    2009-07-01

    Full Text Available Balloon water vapour in situ and remote measurements in the tropical upper troposphere and lower stratosphere (UTLS obtained during the HIBISCUS campaign around 20° S in Brazil in February–March 2004 using a tunable diode laser (μSDLA, a surface acoustic wave (SAW and a Vis-NIR solar occultation spectrometer (SAOZ on a long duration balloon, have been used for evaluating the performances of satellite borne remote water vapour instruments available at the same latitude and measurement period. In the stratosphere, HALOE displays the best precision (2.5%, followed by SAGE II (7%, MIPAS (10%, SAOZ (20–25% and SCIAMACHY (35%, all of which show approximately constant H2O mixing ratios between 20–25 km. Compared to HALOE of ±10% accuracy between 0.1–100 hPa, SAGE II and SAOZ show insignificant biases, MIPAS is wetter by 10% and SCIAMACHY dryer by 20%. The currently available GOMOS profiles of 25% precision show a positive vertical gradient in error for identified reasons. Compared to these, the water vapour of the Reprobus Chemistry Transport Model, forced at pressures higher than 95 hPa by the ECMWF analyses, is dryer by about 1 ppmv (20%.

    In the lower stratosphere between 16–20 km, most notable features are the steep degradation of MIPAS precision below 18 km, and the appearance of biases between instruments far larger than their quoted total uncertainty. HALOE and SAGE II (after spectral adjustment for reducing the bias with HALOE at northern mid-latitudes both show decreases of water vapour with a minimum at the tropopause not seen by other instruments or the model, possibly attributable to an increasing error in the HALOE altitude registration. Between 16–18 km where the water vapour concentration shows little horizontal variability, and where the μSDLA balloon measurements are not perturbed by outgassing, the average mixing ratios reported by the remote sensing instruments are substantially lower than the 4–5

  20. Environ: E00798 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00798 Red sorrel Medicinal herb Citrate [CPD:C00158] [DR:D00037], Malate [CPD:C007... Potassium [CPD:C00238] [DR:D08403] Hibiscus sabdariffa [TAX:183260] Malvaceae (mallow family) Red sorrel calyx Medicinal

  1. ANTI-SYNCHRONIZATION OF PAN AND LIU CHAOTIC SYSTEMS BY ACTIVE NONLINEAR CONTROLNATURAL FIBER BONE PLATES-A WORLDWIDE PATENT SEARCH REPORT

    Directory of Open Access Journals (Sweden)

    Mr. D. CHANDRAMOHAN,

    2011-05-01

    Full Text Available This invention concentrates on the biomaterials progress in the field of orthopaedics. An effort to utilize the advantages offered by renewable resources for the development of biocomposite materials based on bio epoxy resin and natural fibers such as Agave sisalana; Musa sepientum; Hibiscus sabdariffa and its application in bone grafting substitutes.

  2. and spider plant (Cleome gynandra)

    African Journals Online (AJOL)

    User

    2016-02-17

    Feb 17, 2016 ... scarbrum, Solanum american, Solanum nigrum and. Solanum villosum. ..... Productivity and Chemical Constituents of Roselle (Hibiscus sabdariffa L.) ... Shanhua, Taiwan: AVRDC-The World Vegetable Center. Kimiywe J ... and quality attributes of Solanum villosum (black night shade) in. Keiyo district, Rift ...

  3. Review of traditional and non-traditional medicinal genetic resources in the USDA, ARS, PGRCU collection evaluated for flavonoid concentrations and anthocyanin indexes

    Science.gov (United States)

    Non-traditional medicinal species include velvetleaf (Abutilon theophrasti Medik.), Desmodium species, Termanus labialis (L.f.) Spreng. and the traditional species consists of roselle (Hibiscus sabdariffa L.). There is a need to identify plant sources of flavonoids and anthocyanins since they have s...

  4. Erythrocyte Osmotic Fragility and Excitability Score in Rabbit fed ...

    African Journals Online (AJOL)

    olayemitoyin

    protect cells against oxidative stress in rats (Wang et al., 2000) and ... building for easy and effective cross-ventilation, and the cages were ... Graded level of Hibiscus sabdariffa alters erythrocyte membrane stability in rabbit. 114 ..... Haematological and serum biochemical response ... Vitamin C in human health and disease ...

  5. Hibiscus and Wild Flowers

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    Woodcut artist Zheng Shuang convinces the audience through her display of poetic emotion Benefiting from training in watercoior paintings early in life, strict literary sketch training as well, as 10 years study at the Central Academy of Fine Aris, Zheng has mare than 30 years teaching experience at the Ouangzhou Academy of Fine Aris "I"m a roadside grass, a wild flower," Zheng Shuang describes herself She said she often talks to nature-the mountains, the trees, the flowers and the

  6. Calidad seminal en ovinos pelibuey con inclusión de Hibiscus rosa-sinensis en la dieta

    Directory of Open Access Journals (Sweden)

    José Maza Gamboa

    2015-01-01

    Full Text Available La alimentación de los ovinos en el trópico representa del 48 a 90 % de los costos de producción, además, el uso de granos hace dependientes a los ovinocultores del mercado internacional. Ganancias de peso moderadas se han obtenido con follaje de plantas arbustivas, sin embargo, algunas plantas como el Tulipán (Hibiscus rosa-sinensis presentan compuestos que pueden actuar como fito-estrógenos, lo que puede comprometer el uso potencial en la alimentación de sementales ovinos pelibuey. Método: doce sementales ovinos Pelibuey, de 24±3 meses de edad, con un peso de 44±5 kg y una condición corporal de 2.3 +0.3 en una escala de 1 a 5 fueron asignados a 2 tratamientos (n=6 durante 45 días. En dietas con base en pasto de corte (Pennisetum purpureum x P. Typhoides var. CT-115, para el tratamiento 1 (T1, se utilizó alimento comercial (500 g d-1 por ovino con 16% de PC; y para el tratamiento 2 (T2, 20% de Tulipán (451 g MS/ kg MV, ~1.6 kg d-1 por ovino. Al finalizar la prueba se evaluó la calidad seminal, las variables de respuesta fueron: volumen del eyaculado (Vol, ml y a través del Análisis de Semen Asistido por Computadora (CASA la concentración espermática (CE, Millones por ml y ocho parámetros de motilidad individual: velocidad curvilínea (VCL, μ/s, velocidad rectilínea (VSL, μ/s, velocidad promedio (VAP, μ/s, índice de linealidad (LIN, %, índice de rectitud (STR, %, índice de oscilación (WOB, %, amplitud media del desplazamiento lateral de la cabeza (ALH, μ y frecuencia de batido de cola (BCF, Hz. Para determinar las diferencias entre las medias para tratamientos para Vol y CE, VCL, VSL y VAP se utilizó una t de Student; para los parámetros de motilidad individual se realizó una reducción de factores y una clasificación en dos fases de los índices obtenidos para caracterizar las subpoblaciones espermáticas por motilidad. Los análisis fueron realizados con el software SPSS 15.0 para Windows Resultados: no se

  7. Apple, carrot, and hibiscus edible films containing the plant antimicrobials carvacrol and cinnamaldehyde inactivate Salmonella Newport on organic leafy greens in sealed plastic bags.

    Science.gov (United States)

    Zhu, Libin; Olsen, Carl; McHugh, Tara; Friedman, Mendel; Jaroni, Divya; Ravishankar, Sadhana

    2014-01-01

    The objective of this study was to investigate the antimicrobial effects of carvacrol and cinnamaldehyde incorporated into apple, carrot, and hibiscus-based edible films against Salmonella Newport in bagged organic leafy greens. The leafy greens tested included organic Romaine and Iceberg lettuce, and mature and baby spinach. Each leafy green sample was washed, dip inoculated with S. Newport (10⁷ CFU/mL), and dried. Each sample was put into a Ziploc® bag. Edible films pieces were put into the Ziploc bag and mixed well. The bags were sealed and stored at 4 °C. Samples were taken at days 0, 3, and 7 for enumeration of survivors. On all leafy greens, 3% carvacrol films showed the best bactericidal effects against Salmonella. All 3 types of 3% carvacrol films reduced the Salmonella population by 5 log₁₀ CFU/g at day 0 and 1.5% carvacrol films reduced Salmonella by 1 to 4 log₁₀ CFU/g at day 7. The films with 3% cinnamaldehyde showed 0.5 to 3 log reductions on different leafy greens at day 7. The films with 0.5% and 1.5% cinnamaldehyde and 0.5% carvacrol also showed varied reductions on different types of leafy greens. Edible films were the most effective against Salmonella on Iceberg lettuce. This study demonstrates the potential of edible films incorporated with carvacrol and cinnamaldehyde to inactivate S. Newport on organic leafy greens.

  8. Binary solvent extraction system and extraction time effects on phenolic antioxidants from kenaf seeds (Hibiscus cannabinus L.) extracted by a pulsed ultrasonic-assisted extraction.

    Science.gov (United States)

    Wong, Yu Hua; Lau, Hwee Wen; Tan, Chin Ping; Long, Kamariah; Nyam, Kar Lin

    2014-01-01

    The aim of this study was to determine the best parameter for extracting phenolic-enriched kenaf (Hibiscus cannabinus L.) seeds by a pulsed ultrasonic-assisted extraction. The antioxidant activities of ultrasonic-assisted kenaf seed extracts (KSE) were determined by a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay, β -carotene bleaching inhibition assay, and ferric reducing antioxidant power (FRAP) assay. Total phenolic content (TPC) and total flavonoid content (TFC) evaluations were carried out to determine the phenolic and flavonoid contents in KSE. The KSE from the best extraction parameter was then subjected to high performance liquid chromatography (HPLC) to quantify the phenolic compounds. The optimised extraction condition employed 80% ethanol for 15 min, with the highest values determined for the DPPH, ABTS, and FRAP assay. KSE contained mainly tannic acid (2302.20 mg/100 g extract) and sinapic acid (1198.22 mg/100 g extract), which can be used as alternative antioxidants in the food industry.

  9. Inductive effect of the leaf mixture extract of Aloe buettneri, Justicia insularis, Dicliptera verticillata and Hibiscus macranthus on in vitro production of estradiol.

    Science.gov (United States)

    Telefo, P B; Moundipa, P F; Tchouanguep, F M

    2004-04-01

    In the course of a preliminary clarification of the mechanisms of the leaf mixture extract of Aloe buettneri, Justicia insularis, Dicliptera verticillata and Hibiscus macranthus, locally used to regulate the menstrual cycle and to treat dysmenorrhea or cases of infertility in women, pieces of proestrus rat ovary were incubated in the presence of increasing concentration of the plant extract and/or human Chorionic Gonadotropin (hCG). The in vitro production of estradiol and progesterone by ovarian cells of proestrus rat was significantly increased in the presence of various concentration of hCG (P < 0.05). The different concentration of the plant extract increased the production of estradiol by twofold. In addition, the in vitro production of estradiol by ovarian cells increased by 13-fold when they were incubated with hCG (0.1 IU/ml) and a concentration of 130 microg/ml of the plant extract. These results clearly attest the direct effects of some chemical components of the leaf mixture of the plants on ovarian steroidogenesis.

  10. Binary Solvent Extraction System and Extraction Time Effects on Phenolic Antioxidants from Kenaf Seeds (Hibiscus cannabinus L. Extracted by a Pulsed Ultrasonic-Assisted Extraction

    Directory of Open Access Journals (Sweden)

    Yu Hua Wong

    2014-01-01

    Full Text Available The aim of this study was to determine the best parameter for extracting phenolic-enriched kenaf (Hibiscus cannabinus L. seeds by a pulsed ultrasonic-assisted extraction. The antioxidant activities of ultrasonic-assisted kenaf seed extracts (KSE were determined by a 2,2-diphenyl-1-picrylhydrazyl (DPPH radical scavenging capacity assay, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS radical scavenging assay, β-carotene bleaching inhibition assay, and ferric reducing antioxidant power (FRAP assay. Total phenolic content (TPC and total flavonoid content (TFC evaluations were carried out to determine the phenolic and flavonoid contents in KSE. The KSE from the best extraction parameter was then subjected to high performance liquid chromatography (HPLC to quantify the phenolic compounds. The optimised extraction condition employed 80% ethanol for 15 min, with the highest values determined for the DPPH, ABTS, and FRAP assay. KSE contained mainly tannic acid (2302.20 mg/100 g extract and sinapic acid (1198.22 mg/100 g extract, which can be used as alternative antioxidants in the food industry.

  11. Effects of Three Plant Growth Regulators on the Physiological Indices of Hibiscus Rosa-sinensis%3种植物生长调节剂对七彩朱槿生理指标的影响

    Institute of Scientific and Technical Information of China (English)

    玉舒中; 王美英; 李悦; 杨振德; 雷世满; 吕文玲

    2012-01-01

    [目的]探讨植物生长调节剂对七彩朱槿生长影响的生理机制.[方法]采用L16(43)的正交试验设计,研究多效唑( PP333)、矮壮素(CCC)、比久(B9)3种植物生长调节剂对盆栽七彩朱槿叶绿素含量以及POD、CAT、NR的影响.[结果]叶施与根施生长调节剂对七彩朱槿的生理影响效果不同.叶施生长调节剂对叶绿素含量、POD和CAT的影响大于根施;而根施对NR的促进作用大于叶施,含高浓,度CCC(4000mg/L)的(14)、(15)、(16)处理的NR明显高于对照.3种生长调节剂中,CCC对七彩朱槿NR的影响最显著,其中根施CCC处理的影响达极显著水平.[结论]合理使用植物生长调节剂,不仅能起到化学修剪的作用,同时可以增强七彩朱槿的光合作用能力、抗性以及对氮肥的利用能力.%[Objective] The research aimed to explore the physiological mechanism that plant growth regulators made effects on the growth of Hibiscus Rosa-sinensis. [Method] The experiments were conducted as the orthogonal design of L16 (4 ) to study the effects of three plant growth regulators , namely PP333, CCC, B9, on th e chlorophyll content, POD, CAT and NR of Hibiscus Rosa-sinensis grown in pot. [Result] The effects on the physiological indices of Hibiscus Rosa-sinensis between foliar fertilization and root fertilization of PP333 ,CCC and B9 were different. The effects on the chlorophyll content, POD and CAT made by foliage fertilization were more obvious than by root fertilization, but it was opposite to NR, and the values of NR of Hibiscus Rosa-sinensis treated by the (14), (15) and (16) combinations, which all contained CCC in high concentration (4 000 mg/L) ,were significantly higher than CK. Among the three plant growth regulators, CCC had made the most significant effect on NR of Hibiscus Rosa-sinensis, especially by the way of root fertilization, and the difference could reach extremely significant level compared with the others. [Conclusion] Rational use

  12. Neutralisation of Local Haemorrhage Induced by the Saw-Scaled Viper Echis carinatus sochureki Venom Using Ethanolic Extract of Hibiscus aethiopicus L.

    Directory of Open Access Journals (Sweden)

    S. S. Hasson

    2012-01-01

    Full Text Available The objective of the study is to investigate the anti-snake venom activities of a local plant, Hibiscus aethiopicus L. The H. aethiopicus was dried and extracted with ethanol. Different assays were performed according to standard techniques, to evaluate the plant’s acute toxicity and its antivenom activities. The results of evaluating the systemic acute toxicity of the H. aethiopicus extract using “oral and intra-peritoneal” route were normal even at the highest dose (24 g/kg tested. All guinea pigs (n=3 when treated with venoms E. c. sochureki (75 μg alone induced acute skin haemorrhage. In contrast, all guinea pigs (n=18 treated with both venom and the plant extract at a concentration between 500 and 1000 mg/kg showed no signs of haemorrhage. Moreover, all guinea pigs (n=18 treated with venom and the plant extract below 400 mg/kg showed acute skin haemorrhage. All guinea pigs treated with venom E. c. sochureki (75 μg alone induced acute skin haemorrhage after both 24 and 32 hours. In contrast, all guinea pigs treated with both venom and the plant extract (administered independently at concentrations between 500 and 1000 mg/kg showed no signs of haemorrhage after 32 hours. However, after 24 hours all tested guinea pigs showed less inhibition (<60% compared to that obtained after 32 hours. The outcome of this study reflects that the extract of H. aethiopicus plant may contain an endogenous inhibitor of venom induced local haemorrhage.

  13. Cognitive enhancing and antioxidant activity of ethyl acetate soluble fraction of the methanol extract of Hibiscus rosa sinensis in scopolamine-induced amnesia

    Directory of Open Access Journals (Sweden)

    Vandana S Nade

    2011-01-01

    Full Text Available Objective : The objective of the present study was to evaluate the cognitive enhancing and antioxidant activity of Hibiscus rosa sinensis. Materials and Methods : The learning and memory was impaired by administration of scopolamine (1 mg/kg, i.p. in mice which is associated with altered brain oxidative status. The object recognition test (ORT and passive avoidance test (PAT were used to assess cognitive enhancing activity. Animals were treated with an ethyl acetate soluble fraction of the methanol extract of H. sinensis (25, 50 and 100 mg/kg, p.o. Results : The ethyl acetate soluble fraction of the methanol extract of H. sinensis (EASF attenuated amnesia induced by scopolamine and aging. The discrimination index (DI was significantly decreased in the aged and scopolamine group in ORT. Pretreatment with EASF significantly increased the DI. In PAT, scopolamine-treated mice exhibited significantly shorter step-down latencies (SDL. EASF treatment showed a significant increase in SDL in young, aged as well as in scopolamine-treated animals. The biochemical analysis of brain revealed that scopolamine treatment increased lipid peroxidation and decreased levels of superoxide dismutase (SOD and glutathione reductase (GSH. Administration of extract significantly reduced LPO and reversed the decrease in brain SOD and GSH levels. The administration of H. sinensis improved memory in amnesic mice and prevented the oxidative stress associated with scopolamine. The mechanism of such protection of H. sinensis may be due to augmentation of cellular antioxidants. Conclusion : The results of the present study suggested that H. sinensis had a protective role against age and scopolamine-induced amnesia, indicating its utility in management of cognitive disorders.

  14. Basic amino acid mutations in the nuclear localization signal of hibiscus chlorotic ringspot virus p23 inhibit virus long distance movement.

    Science.gov (United States)

    Gao, Ruimin; Wong, Sek-Man

    2013-01-01

    The p23 is a unique protein in the Hibiscus chlorotic ringspot virus which belongs to Family Tombusviridae Genus Carmovirus. Our previous results showed that the p23 is indispensable for host-specific replication and is localized in the nucleus with a novel nuclear localization signal. To investigate additional function(s) of p23, mutations of basic amino acids lysine (K), arginine (R) and histidine (H) that abolish its nuclear localization, were introduced into a biologically active full-length cDNA clone p223 of HCRSV for testing its effects on virus replication and virus movement in vivo. Primer-specific reverse transcription-PCR was conducted to detect gene transcript level of p23 and viral coat protein separately. Virus replication and its coat protein expression were detected by fluorescent in situ hybridization and Western blot, respectively. The effect of p23 was further confirmed by using artificial microRNA inoculation-mediated silencing. Results showed that the two mutants were able to replicate in protoplasts but unable to move from inoculated leaves to newly emerged leaves. Both the p23 and the CP genes of HCRSV were detected in the newly emerged leaves of infected plants but CP was not detected by Western blot and no symptom was observed on those leaves at 19 days post inoculation. This study demonstrates that when p23 is prevented from entering the nucleus, it results in restriction of virus long distance movement which in turn abrogates symptom expression in the newly emerged leaves. We conclude that the p23 protein of HCRSV is required for virus long distance movement.

  15. Basic amino acid mutations in the nuclear localization signal of hibiscus chlorotic ringspot virus p23 inhibit virus long distance movement.

    Directory of Open Access Journals (Sweden)

    Ruimin Gao

    Full Text Available The p23 is a unique protein in the Hibiscus chlorotic ringspot virus which belongs to Family Tombusviridae Genus Carmovirus. Our previous results showed that the p23 is indispensable for host-specific replication and is localized in the nucleus with a novel nuclear localization signal. To investigate additional function(s of p23, mutations of basic amino acids lysine (K, arginine (R and histidine (H that abolish its nuclear localization, were introduced into a biologically active full-length cDNA clone p223 of HCRSV for testing its effects on virus replication and virus movement in vivo. Primer-specific reverse transcription-PCR was conducted to detect gene transcript level of p23 and viral coat protein separately. Virus replication and its coat protein expression were detected by fluorescent in situ hybridization and Western blot, respectively. The effect of p23 was further confirmed by using artificial microRNA inoculation-mediated silencing. Results showed that the two mutants were able to replicate in protoplasts but unable to move from inoculated leaves to newly emerged leaves. Both the p23 and the CP genes of HCRSV were detected in the newly emerged leaves of infected plants but CP was not detected by Western blot and no symptom was observed on those leaves at 19 days post inoculation. This study demonstrates that when p23 is prevented from entering the nucleus, it results in restriction of virus long distance movement which in turn abrogates symptom expression in the newly emerged leaves. We conclude that the p23 protein of HCRSV is required for virus long distance movement.

  16. Temperature- and Relative Humidity-Dependent Life History Traits of Phenacoccus solenopsis (Hemiptera: Pseudococcidae) on Hibiscus rosa-sinensis (Malvales: Malvaceae).

    Science.gov (United States)

    Chen, H S; Yang, L; Huang, L F; Wang, W L; Hu, Y; Jiang, J J; Zhou, Z S

    2015-08-01

    Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae), a worldwide distributive invasive pest, originated from the United States, and it was first reported in Guangdong province, China, in 2008. The effects of temperature and relative humidity (RH) on the life history traits of P. solenopsis on Hibiscus rosa-sinensis L. (Malvales: Malvaceae) were studied at seven constant temperatures (15, 20, 25, 27.5, 30, 32.5, and 35°C) and three RHs (45, 60, and 75%). The results showed that temperature, RH, and their interactions significantly influenced the life history traits of P. solenopsis. First instar was the most sensitive stage to extreme temperatures with very low survival rates at 15 and 35°C. At 25-32.5°C and the three RHs, the developmental periods of entire immature stage were shorter with values between 12.5-18.6 d. The minimum threshold temperature and the effective accumulative temperature for the pest to complete one generation were 13.2°C and 393.7 degree-days, respectively. The percentage and longevity of female adults significantly differed among different treatments. It failed to complete development at 15 or 35°C and the three RHs. Female fecundity reached the maximum value at 27.5°C and 45% RH. The intrinsic rate for increase (r), the net reproductive rate (R0), and the finite rate of increase (λ) reached the maximum values at 27.5°C and 45% RH (0.22 d(-1), 244.6 hatched eggs, and 1.25 d(-1), respectively). Therefore, we conclude that 27.5°C and 45% RH are the optimum conditions for the population development of the pest.

  17. Bacillus amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for the production of Bikalga, an alkaline fermented food

    DEFF Research Database (Denmark)

    Compaor, C.S.; Nielsen, D.S.; Sawadogo-Lingani, H.

    2013-01-01

    Aims: To identify and screen dominant Bacillus spp. strains isolated from Bikalga, fermented seeds of Hibiscus sabdariffa for their antimicrobial activities in brain heart infusion (BHI) medium and in a H. sabdariffa seed-based medium. Further, to characterize the antimicrobial substances produced....... They produced several lipopeptide antibiotics and showed good potential for biological control of Bikalga. Significance and Impact of the Study: Pathogenic bacteria often occur in spontaneous food fermentations. This is the first report to identify indigenous B. amyloliquefaciens ssp. plantarum strains...

  18. Study on the Germination of the Treated Hibiscus tiliaceus Seeds%人工处理黄槿种子萌发的研究

    Institute of Scientific and Technical Information of China (English)

    严廷良; 刘强

    2012-01-01

    Hibiscus tiliaceus L. Seeds were treated by different concentrations of Gibberellin(GA) and Salicylic acid (SA) and placed in different soil types for germination. Seed germination was observed in pot experiment and field experiment. The results showed that the germination rate was 38.0 % and the germination potential was 35.3 % in the original soil when the seeds were treated by 200 Μl/L GA. The germination rate was 42.6% and the germination potential was 32.0% in the original soil when the seeds were treated by 400 Μl/L SA. In the pot experiment, both germination rate and germination potential reached the highest at the GA concentration of 20 Μl/L, the germination rate was 39.4% and the germination potential was 36.0%. The germination rate and the germination potential were both the highest value at the SA concentration of 10 u,L/L, the germination rate was 30.6% and the germination potential was 22.7%. In the field experiment, the germination rate and the germination potential reached the highest at the concentration of 400 Μl/L salicylic acid and 400 Μl/L GA, the germination rate was 26.5% and the germination potential was 18.3% for the GA treatment. The germination rate was 33.5% and the germination potential was 26.7% for the SA treatment. The best conditions for the hibiscus seed germination were obtained through the experiments. It provided the technical reference for the coastal forest construction of Casuarina equisetifolia L. Mixed planting with H. Tiliaceus.%采用不同体积分数的赤霉素、水杨酸溶液处理黄槿种子之后,将种子播入不同的土壤基质中,从野外林地试验和盆栽试验中观察种子的萌发情况.结果表明,在原土盆栽试验中,体积分数为200μL/L的赤霉素处理可使发芽率达到38.0%、发芽势达到35.3%;400 μL/L的水杨酸处理可使发芽率达到42.6%、发芽势达到32.0%.在沙土盆栽试验中,20 μL/L的赤霉素处理可使发芽率和发芽势

  19. Anatomical factors predicting lower calyceal stone clearance after ...

    African Journals Online (AJOL)

    M. Khan

    2016-02-17

    Feb 17, 2016 ... Conclusions: This study shows that lower infundibular length and width are ... Stone disease is endemic in Pakistan and constitutes 60% of the ... lithotripsy session by plane X-ray abdomen and renal ultrasound. ... Pain was.

  20. Factors affecting lower calyceal stone clearance after Extracorporeal ...

    African Journals Online (AJOL)

    shanker

    different methods of measuring the angle, the use of different types of lithotripters, or ... A P-value <0.05 was considered statistically significant. Results .... References. [1] Drach GW, Dretler S, Fair W, Finlayson B, Gillenwater J, Griffith D, et al.

  1. Anatomical factors predicting lower calyceal stone clearance after ...

    African Journals Online (AJOL)

    Persistent stone fragments ≥6 months after the completion of treatment was defined as ... SPSS version 15.0 was utilized for all statistical analysis. ... Infundibulopelvic (IP) angle had no statistically significant effect on stone-free rate (p = 0.1).

  2. Nutritive and Antioxidant properties of Shade Dried Leafy Vegetables Consumed in Northern Côte d’Ivoire

    OpenAIRE

    Patricia Dehegnan Oulaï; Lessoy Yves Thierry Zoue; Sébastien Lamine Niamké

    2016-01-01

    Leafy vegetables are highly perishable and often subjected to post-harvest wastes. Among the various methods of preservation, the effect of shadow drying on the nutritive value and antioxidant properties of five (5) leafy vegetables species widely consumed in Northern Côte d’Ivoire was investigated. These species were Amaranthus hybridus, Andasonia digitata, Ceiba patendra, Hibiscus sabdariffa and Vigna unguiculata. Experiment was conducted as follow: portions of washed and drained fresh leaf...

  3. Quality and Antioxidant Activity of Yogurt Supplemented with Roselle during Cold Storage

    OpenAIRE

    El Latifa Sri Suharto; Irma Isnafia Arief3); Epi Taufik

    2016-01-01

    The purpose of this study was to determine the quality (physical, chemical, microbiological characteristics), total phenolic content, and antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) inhibition assay of probiotic yogurt supplemented with roselle flower extract (Hibiscus sabdariffa L) during cold storage. The experiment used treatment for types of yogurt as follows: cow’s milk probiotic yogurt + roselle, goat’s milk probiotic yogurt + roselle, cow’s milk yogurt, and ...

  4. (Hibiscus cannabinus) accessions in Nigeria

    African Journals Online (AJOL)

    morufat

    2013-05-08

    May 8, 2013 ... (Balogun et al., 2007) or controlled illumination in vivo has been used ... Fertilizer (N-P-K 15-15-15) was applied ... Controlled environment studies: In vitro ..... Proprietary Software Release 8.2 (TS2M0), SAS Institute Inc., Cary,.

  5. Mechanical properties of natural fibre reinforced polymer composites

    Indian Academy of Sciences (India)

    A S Singha; Vijay Kumar Thakur

    2008-10-01

    During the last few years, natural fibres have received much more attention than ever before from the research community all over the world. These natural fibres offer a number of advantages over traditional synthetic fibres. In the present communication, a study on the synthesis and mechanical properties of new series of green composites involving Hibiscus sabdariffa fibre as a reinforcing material in urea–formaldehyde (UF) resin based polymer matrix has been reported. Static mechanical properties of randomly oriented intimately mixed Hibiscus sabdariffa fibre reinforced polymer composites such as tensile, compressive and wear properties were investigated as a function of fibre loading. Initially urea–formaldehyde resin prepared was subjected to evaluation of its optimum mechanical properties. Then reinforcing of the resin with Hibiscus sabdariffa fibre was accomplished in three different forms: particle size, short fibre and long fibre by employing optimized resin. Present work reveals that mechanical properties such as tensile strength, compressive strength and wear resistance etc of the urea–formaldehyde resin increases to considerable extent when reinforced with the fibre. Thermal (TGA/DTA/DTG) and morphological studies (SEM) of the resin and biocomposites have also been carried out.

  6. 4种真菌对木槿棉蚜毒力作用的研究%Study on Virulence of 4 Fungus Species on Aphis gossypii at Hibiscus syriacus plants

    Institute of Scientific and Technical Information of China (English)

    汪文凯

    2014-01-01

    In the tests strains of 4 fungus species including P.c, Pfu02, B and Ma4 were cultured on SDAY solid culture media, and suspension liquids were prepared separately with the conidia got in the culture. A comparative study was made on virulence of the suspension liquids on Aphis gossypii at Hibiscus syriacus plants indoors and outdoors by spraying. The test results showed that all the 4 fungus species had good indoor virulence on Aphis gossypii at Hibiscus syriacus plants and the optimal one was Strain B with the lethality rate reaching 100% while Strain Ma4 had the lowest lethality rate of 63.28%. It was also found that the strains had definite inhibiting effects on the fecundity of Aphis gossypii indoors. But due to the influence of environmental factors,the fungus strains had poor outdoor control effects on Aphis gossypii and the best was Strain B with the lethality rate being only 7.94%. The test results indicated that the fungi had good indoor application effects as microbial insecticides,but had limitations in outdoor application.%本试验利用SDAY固体培养基对P.c、Pfu02、B、Ma44种真菌菌株进行培养,用培养所得的各种分生孢子分别配制成悬浮液,采用喷雾法在室内、室外对木槿上棉蚜的毒力作用进行了比较研究。试验结果表明:4种真菌菌株在室内对木槿棉蚜均有较好的毒力作用,最理想的是菌株B,致死效果达到了100%,最差的是菌株Ma4,也达到了63.28%,同时发现对棉蚜的繁殖力有一定的抑制效果。但在室外由于环境因素的影响,对棉蚜的控制作用很差,最好的菌株B对蚜虫的致死率也只有7.94%。该试验表明,各真菌作为微生物杀虫剂在室内施用时效果理想,而在室外施用时受到一定的局限性。

  7. Measurement of large nonlinear refractive index of natural pigment extracted from Hibiscus rosa-sinensis leaves with a low power CW laser and by spatial self-phase modulation technique

    Science.gov (United States)

    Biswas, S.; Kumbhakar, P.

    2017-02-01

    We have reported here, for the first time, to the best of our knowledge, a high nonlinear refractive index (n2e) of a natural pigment extracted from Hibiscus rosa-sinensis leaves by using spatial self-phase modulation technique (SSPM) with a low power CW He-Ne laser radiation at 632.8 nm. It is found by UV-Vis absorption spectroscopic analysis that chlrophyll-a, chlrophyll-b and carotenoid are present in the pigment extract with 56%, 25% and 19%, respectively. The photoluminescence (PL) emission characteristics of the extracted samples have also been measured at room temperature as well as in the temperature range of 283-333 K to investigate the effect of temperature on luminescent properties of the sample. By analyzing the SSPM experimental data, the nonlinear refractive index value of pigment extract has been determined to be 3.5 × 10- 5 cm2/W. The large nonlinear refractive index has been assigned due to asymmetrical structure, molecular reorientation and thermally induced nonlinearity in the sample. The presented results might open new avenues for the green and economical technique of syntheses of organic dyes with such a large nonlinear optical property.

  8. 黄槿内生真菌的次级代谢产物及其生物活性研究%Secondary metabolites and their bioactivities of Hibiscus tiliaceus L.endophytic fungi

    Institute of Scientific and Technical Information of China (English)

    李丹; 朱天骄; 顾谦群; 李德海

    2012-01-01

    目的 对1株分离自广西红树林植物黄槿(Hibiscus tiliaceus)的内生真菌Penicillium sp.LD-68的抗肿瘤活性成分进行研究.方法 采用溶剂萃取、柱色谱层析及制备HPLC等方法对菌株发酵产物进行活性追踪分离,通过理化性质及波谱学手段进行化学结构鉴定,以SRB和MTT法评价化合物的抗肿瘤活性.结果 从中分离得到5个弯孢霉菌素类化合物,其结构分别鉴定为curvularin(1)、dehydrocurvularin(2)、11-β-Hydroxy-12-Oxocurvularin(3)、11-β-Hydroxycurvularin(4)、11-α-Hydroxycurvularin(5).化合物1~5对A549、hela、BEL-7402、K562 4种人肿瘤细胞株具有不同程度的抑制作用,其中化合物2、3、4、5对hela细胞具有较好的抗肿瘤细胞活性,IC50分别为3.99、7.75、10.00、5.10 μmol·L-1.结论 首次测定了化合物3~5对A549、hela、BEL-7402、K562 4种肿瘤细胞系的细胞毒活性,其中对hela细胞显示出较高的抑制率.%Objective To investigate the antitumor bioactive ingredient of a endophytic fungus Penicillium sp. LD-68 isolated from Guang Xi mangrove plant Hibiscus tiliaceus L.. Methods The extract of the fungus was purified by solvent extraction, column chromatography and preparative HPLC guided by the bioassay results. Their structures were established by physicochemical properties and spectroscopic a-nalysis. The antitumor activities were evaluated by SRB method and MTT method. Results Five compounds were identified as curvularin (1), dehydrocurvularin (2), ll-β-Hydroxy-12-Oxocurvularin (3), ll-β-Hydroxycurvularin (4), 11-α-Hydroxycurvularin (5). Compounds 1-5 showed inhibitory activities to four kinds of human tumor cell lines including A549, hela, BEL-7402 and K562, and compounds 2~5 have more potential antitumor activities against hela cells, with IC50 values of 3. 99, 7. 75, 10. 00 and 5.10μM, respectively. Conclusion Compounds 3~5 are reported their cytoxic activities against the four kinds of human tumor cell lines

  9. Effects of Roselle and Ginger on cisplatin-induced reproductive toxicity in rats

    Institute of Scientific and Technical Information of China (English)

    Amr Amin; AlaaEldin A. Hamza

    2006-01-01

    Aim: To evaluate the protective effects of Hibiscus sabdariffa (Roselle) and Zingiber officinale (Ginger) against cisplatin-induced reproductive toxicity in rats and to study the mechanisms underlying these effects. Methods:which began 21 days before a single cisplatin I.p. Injection (10 mg/kg body weight). Results: Extracts of H. Sabdariffa and Z. Officinale reduced the extent of cisplatin-induced sperm abnormality and enhanced sperm motility. Both extracts restored the control level of malondialdehyde (MDA) (lipid peroxidation marker) in the cisplatin-treated testis.The cisplatin injection induced decline in the levels of superoxide dismutase (SOD), reduced glutathione (GSH) and catalase (CAT) were significantly reversed to control levels in groups where cisplatin was preceded by the administration of either H. Sabdariffa or Z. Officinale. Conclusion: Both H. Sabdariffa and Z. Officinale treatment increased the activities of testicular antioxidant enzymes and restored sperm motility of cisplatin-treated rats. The protective effects of tested plants are, therefore, suggested to be mediated by their potent antioxidant activities.

  10. 红麻Ms5基因的克隆及多转录本分析%Cloning and multiple transcript analysis of Ms5 gene in kenaf(Hibiscus canabius L.)

    Institute of Scientific and Technical Information of China (English)

    唐向民; 金刚; 李刚; 周琼; 陈鹏; 周瑞阳

    2012-01-01

    The full mRNA of kenaf Ms5 gene was tried to clone with RT-PCR and 3'RACE methods based on the known partial 5'-end cDNA sequence. The sequencing results showed that two transcripted cDNAs of Ms5 gene were cloned from the anther of kenaf. The length of two transcripts named as Hc-Ms5~α and HcMs5-β were 972 bp and 1 105 bp,respectively. The structure analysis showed that both of them contained an 858 bp ORF,encoding a protein of 285 amino acids with predicted molecular weight of 32. 4 ku and a isoelectric point of 7. 62. The sequencing results showed that 5 -end 972 bp sequence of the two transcripts was identical,but HcMs5~β possessed an additional 133 bp sequence at 3'-end after 972 bp compared with HcMs5-α. The alternative termination of transcription in the 3'UTR of kenaf Ms5 maybe related with the post transcriptional regulation of the gene. It is the first report about the multiple transcripts of Ms5 gene in higher plant.%为研究Ms5基因在红麻(Hibiscus canabius L.)花药中的转录特征,以L23A不育系为试材,基于已知的红麻Ms5基因5 ′端cDNA序列,采用3′RACE和RT-PCR技术对该基因全长cDNA序列进行克隆.序列比对分析结果表明,红麻花药组织中同时存在Ms5基因2种不同的转录本,其长度分别为972和1105 bp,依次命名为HcMs5-α和HcMs5 -β.这2种转录本均包含了完整且一致的编码序列,其编码序列(CDS)全长为858bp,预测编码的蛋白质含有285个氨基酸残基,分子质量为32.4 ku,理论等电点为7.62;两者5 ′端前972 bp序列完全一致,但3′端的非编码区(UTR)长度有所不同,HcMs5-β在第972 bp以后比HcMs5-α多出133 bp的序列,具有选择性转录终止的特征.这种转录终止信号的可选择性可能与该基因转录后水平的调控有关.

  11. POST-EXERCISE REHYDRATION WITH DIFFERENT BEVERAGES: PURE WATER, SPORTS DRINK, AND ROSELLE TEA

    OpenAIRE

    María de Lourdes Mayol Soto; Luis Fernando Aragón Vargas

    2002-01-01

    The purpose of the present study was to assess the suitability of roselle tea (Hibiscus Sabdariffa L.) for post-exercise rehydration, compared with pure water and a sports drink. Roselle tea is a beverage widely consumed in Mexico and highly regarded because of its purported diuretic properties. Sixteen physically active male students from the University of Costa Rica, aged 17 to 23 years old, drank each one of the three treatments on different days, the same day of the week, in random order....

  12. Impact test on natural fiber reinforced polymer composite materials

    Directory of Open Access Journals (Sweden)

    D. Chandramohan

    2013-06-01

    Full Text Available In this research, natural fibers like Sisal (Agave sisalana, Banana (Musa sepientum & Roselle (Hibiscus sabdariffa , Sisal and banana (hybrid , Roselle and banana (hybrid and Roselle and sisal (hybrid are fabricated with bio epoxy resin using molding method. In this work, impact strength of Sisal and banana (hybrid, Roselle and banana (hybridand Roselle and sisal (hybrid composite at dry and wet conditions were studied. Impact test were conducted izod impact testing machine. In this work micro structure of the specimens are scanned by the Scanning Electron Microscope.

  13. Twenty natural organic pigments for application in dye sensitized solar cells

    Science.gov (United States)

    Castillo, D.; Sánchez Juárez, A.; Espinosa Tapia, S.; Guaman, A.; Obregón Calderón, D.

    2016-09-01

    In this work we present the results of a study of twenty natural pigments obtained from plants and insects from southern Ecuador. Many of them will be considered as a potential natural sensitizer for the construction of DSSCs. The results indicate that these pigments have a good performance in the absorbance and wavelength spectra. Were selected four best pigments for the construction of DSSCs, Rumex tolimensis Wedd, Raphanus sativus, Hibiscus sabdariffa, and Prunus serótina, however the conversion efficiency is lower than 1%.

  14. Analysis of aroma compounds of Roselle by Dynamic Headspace Sampling using different preparation methods

    DEFF Research Database (Denmark)

    Juhari, Nurul Hanisah Binti; Varming, Camilla; Petersen, Mikael Agerlin

    2015-01-01

    The influence of different methods of sample preparation on the aroma profiles of dried Roselle (Hibiscus sabdariffa) was studied. Least amounts of aroma compounds were recovered by analysis of whole dry calyxes (WD) followed by ground dry (GD), blended together with water (BTW), and ground...... and then mixed with water (GMW). The highest number of aroma compounds was found in Roselle treated in water bath (2hr/40°C) (GMWKB). GMW was chosen as the preparation method because it was shown to be an efficient extraction method without the possibility of excessive chemical changes of the sample....

  15. Preliminary characterization of residual biomass from Hibiscus ...

    African Journals Online (AJOL)

    Aghomotsegin

    Generally, industrial juice extraction processes are designed to avoid the extraction ... determination of cellulose, hemicellulose and lignin content together with ...... Techno-economical study of biogas production improved by steam explosion ...

  16. Hibiscus cannabinus feruloyl-coa:monolignol transferase

    Energy Technology Data Exchange (ETDEWEB)

    Wilkerson, Curtis; Ralph, John; Withers, Saunia; Mansfield, Shawn D.

    2016-11-15

    The invention relates to isolated nucleic acids encoding a feruloyl-CoA:monolignol transferase and feruloyl-CoA:monolignol transferase enzymes. The isolated nucleic acids and/or the enzymes enable incorporation of monolignol ferulates into the lignin of plants, where such monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate. The invention also includes methods and plants that include nucleic acids encoding a feruloyl-CoA:monolignol transferase enzyme and/or feruloyl-CoA:monolignol transferase enzymes.

  17. 朱槿花苞提取物治疗皮肤疖痈溃疡及其机制研究%Research in skin healing and repair function and mechanism of Hibiscus rosa-sinensis Linn bud extract

    Institute of Scientific and Technical Information of China (English)

    姜霁洋; 徐涵; 白雪婷; 程訸; 林艳玲; 凌震; 陈一村; 石刚刚

    2015-01-01

    目的:研究朱槿花苞提取物对小鼠皮肤疖痈溃疡模型的治疗作用,并对其可能作用机制进行探讨。方法将昆明小鼠分为模型组、阳性药组和朱槿花苞正丁醇萃取物( HrBN)组,采用小鼠疖痈模型,观察给药后实验结果。将昆明小鼠分为模型组、阳性药组和HrBN低、中、高剂量组,采用小鼠全层切除模型,观察给药后实验结果。通过体外抗菌实验检测朱槿花苞提取物抗菌MIC和抑菌圈。通过碳粒廓清实验测定朱槿花苞提取物对小鼠巨噬细胞廓清作用的影响,计算 K 和α值。结果 HrBN 对疖痈溃疡修复效果明显,并呈现量效关系。 HrBN、朱槿花苞乙酸乙酯萃取物( HrBE)及AB-8树脂体积分数0.3乙醇段、0.6乙醇段的抗菌作用明显。 HrBN及朱槿花苞乙醇提物( HrBA)高剂量能够增加K和α值。结论 HrBN在动物实验中体现了良好的皮肤疖痈溃疡修复作用,其疗效与抗菌活性和免疫增强作用可能有关。%Aim To test the skin healing and repairing efficacy and the mechanism of Hibiscus rosa-sinensis L bud extract by using the animal models. Methods KM mice were randomly divided into three groups:the model group, the positive control group, and the n-bu-tyl alcohol extract ( HrBN) group. Using the boils and carbuncles model, the healing condition of all the animals were observed. KM mice were kept in the SPF condition room and divided into five groups: the model group, the positive control group, and the low, middle, high dose groups. Using the full-thickness loss model, the repairing results of all the mice were ob-served. Through the antimicrobial test, the results of MIC and inhibition zone were obtained. The carbon clearance test was used to collect the blood at the time 5min and 15min, and get the liver and spleen, and the results of K andαwere obtained. Results In vivo ex-periments showed there was significant difference be-tween groups;the Hr

  18. Management of calyceal diverticular calculi: a comparison of percutaneous nephrolithotomy and flexible ureterorenoscopy.

    Science.gov (United States)

    Bas, Okan; Ozyuvali, Ekrem; Aydogmus, Yasin; Sener, Nevzat Can; Dede, Onur; Ozgun, Serhat; Hizli, Fatih; Senocak, Cagri; Bozkurt, Omer Faruk; Basar, Halil; Imamoglu, Abdurrahim

    2015-04-01

    To compare the outcomes in patients who have been treated with flexible ureterorenoscopy (f-URS) and percutaneous nephrolithotomy (PNL) in managing stone-bearing caliceal diverticula. Between April 2007 and October 2013, we performed a retrospective analysis of 54 evaluable patients (28 women and 26 men) with symptomatic stone-bearing caliceal diverticula, who underwent PNL (n = 29) or F-URS (n = 25) in four referral hospitals in Turkey. The groups were compared with respect to demographics, stone location/size, success rate, stone-free status, symptom-free status, complication rates, and hospital stay. The average stone burden preoperatively was significantly larger in patients who were treated with PNL, with the average size for f-URS being 154 ± 77 mm(2) and that for PNL being 211 ± 97 mm(2) (p = 0.023). Symptom-free rates, success rates, stone-free rates and clinically insignificant residual fragments were similar between the groups (p = 0.880 vs. p = 0.537 vs. p = 0.539, and p = 0.877, respectively). There was no statistical difference between the groups for minor complications (p = 0.521) but no major complication (Clavien III-V) occured in the f-URS group; although there were three major complications (10.3 %) (Clavien III) in the PNL group (p PNL group (p PNL. The f-URS procedure is advantageous with respect to a shorter hospital stay and absence of major complications. Therefore, it should be emphasized that the location of the stone and diverticula is an important factor for the selection of the procedure.

  19. Renal pelvis rupture in a kidney with ureteropelvic junction obstruction and extrarenal calyces.

    Science.gov (United States)

    Ransford, George; Young, Ezekiel; Castellan, Miguel; Labbie, Andrew

    2013-06-01

    The extrarenal calyx (ERC) is a rare congenital anomaly, associated with various other abnormalities of the urogenital system. We report a unique case of ERC in a solitary functioning kidney with a massively dilated pelvis that developed spontaneous rupture. A dismembered pyeloplasty was performed at the time of rupture. The patient did well post-operatively with a stable creatinine and stable SFU grade 2 hydronephrosis.

  20. 海洋红树林植物黄槿内生真菌Aspergillus sydowii EN-198化学成分研究%Chemical constituents of Aspergillus sydowii EN-198, an endophytic fungus derived from the marine-mangrove plant Hibiscus tiliaceus

    Institute of Scientific and Technical Information of China (English)

    杜丰玉; 李晓明; 李春顺; 王斌贵

    2012-01-01

    对采自海南东寨港的海洋红树林植物黄瑾(Hibiscus tiliaceus)叶中分离到的一株内生真菌Aspergillus sydowii EN-198的次生代谢产物进行了研究.利用正相与反相硅胶柱层析、葡聚糖凝胶Sephadex LH-20柱层析以及制备薄层层析(PTLC)等分离手段从其发酵液中分离得到14个化合物,通过一维、二维核磁共振技术、质谱技术等鉴定了所有化合物的结构,分别为:环-(S-脯氨酸-S-苯丙氨酸)(1),环-(S-脯氨酸-S-亮氨酸)(2),环-(S-苯丙氨酸-S-色氨酸)(3),(1S)-1-(4 '-间羟基苯甲酸)-1,1,5,5-二甲基己二醇(4),曲酸(5),N-[2-(4-吲哚)乙基]乙酰胺(6),N-[2-(4-对羟基苯酚)乙基]乙酰胺(7),胸腺嘧啶脱氧核苷(8),尿嘧啶脱氧核苷(9),尿嘧啶核苷(10),过氧化麦角甾醇(11),麦角甾醇(12),(2S,2'R,3R,3 'E,4E,8E)-N-(2’-羟基-3’-十六烯酰基)-9-甲基-4,8-二十碳二烯-1,3-二醇(13)以及1-O-β-D-葡萄糖基-(2S,2'R,3R,3'E,4E,8E)-N-(2’-羟基-3’-十六烯酰基)-9-甲基-4,8-二十碳二烯-1,3-二醇(14);其中化合物1和2为首次从Aspergillus sydowii中分离得到.对所有化合物测试其抑菌活性,发现化合物5与7对金黄色葡萄球菌有较好抑制活性.%Cultivation of the fungal strain Aspergillus sydowii EN-198, an endophytic fungus which was isolated from the healthy fresh leaf of marine-mangrove plant Hibiscus tiliaceus, resulted in the identification of fourteen compounds. These compounds were isolated by a combination of silica gel, Sephadex LH-20, and Lobar LiChro-prep RP-18 column chromatography as well as the preparative thin layer chromatography (PTLC). The structures were elucidated by analysis of their spectroscopic data including MS, 1D and 2D NMR to be L,L-cyclo(phenylalanyl-prolyl) (1), L,L-cyclo(leucyl-prolyl) (2), L,L-cyclo(phenylalanyl-tryptophyl) (3), Hydroxy-sydonic acid (4), Kojic acid (5), N-Acetyltryptamine (6), N-[2-(4-Hydroxyphenyl)ethyl]acetamide (7), Thymidine (8), 2'-Deoxyuridine (9

  1. Carbon density and allocation ofBruguiera sexangula andHibiscus tiliaceus mangrove ecosystem in Qinglan harbor of Wenchang city, Hainan province%海南文昌清澜港海莲-黄槿生态系统碳密度及分配格局

    Institute of Scientific and Technical Information of China (English)

    林慧; 曾思齐; 王光军; 梁定栽; 余志金; 李茂金

    2015-01-01

    Vegetation biomass, carbon density and its spatial distribution characteristics ofBruguiera sexangula andHibiscus tiliaceus mangrove ecosystem in Qinglan Barbor of Wenchang city, Hainan province by using Komiyama mangrove allometric growth model. The results show that the total vegetation layer biomass of the mangrove ecosystem was 389.57± 12.73 t/hm2, and of them the tree layer biomass was 387.75±12.01 t/hm2, accounting for 99.5% of the total carbon density of forest vegetation layer; Total organic carbon density of the mangrove ecosystem was 688.51±45.69 t/hm2, of them the vegetation layer carbon capacity per unit area was 184.5 t·hm-2, accounting for 26.6% of the total carbon reserves; 0~105 cm soil organic carbon of per unit area was 504.01±39.69 t·hm-2, accounting for 73.2% of the total ecosystem carbon density, the soil organic carbon sum in undergrowth layer and existing litters layer accounted for only 0.2%.%采用Komiyama红树林异速生长模型,对海南文昌清澜港海莲-黄槿生态系统的植被生物量、碳密度及其空间分布特征进行研究。研究结果表明:海莲-黄槿植被层总生物量为389.57±12.73 t/hm2,其中,乔木层生物量为387.75±12.01 t/hm2,占林分植被层总碳密度的99.5%;海莲-黄槿生态系统总有机碳库密度为688.51±45.69 t/hm2,其中,群落植被层单位面积的碳贮量为184.5 t/hm2,占总碳贮量的26.6%;0~105 cm土壤有机碳单位面积的贮量为504.01±39.69 t/hm2,占生态系统总碳密度的73.2%;林下植被层和现存凋落物层仅占0.2%。

  2. Influence of Cellulose on the Mechanical and Thermal Stability of ABS Plastic Composites

    Directory of Open Access Journals (Sweden)

    K. Crews

    2016-01-01

    Full Text Available Microcrystalline cellulose was explored as possible biodegradable fillers in the fabrication of ABS plastic composites. TGA indicates that upon inclusion of cellulose microcrystals the thermal stability of the ABS plastics was improved significantly when compared to the neat ABS plastic counterparts. Furthermore, inclusion of extracted cellulose from plant biomass showed a higher thermal stability with maximum decomposition temperatures around 131.95°C and 124.19°C for cellulose from cotton and Hibiscus sabdariffa, respectively, when compared to that of the purchased cellulose. In addition, TMA revealed that the average CTE value for the neat ABS and 1 : 1 ratio of cellulose to ABS fabricated in this study was significantly lower than the reported CTE (ca. 73.8 μm/m°C.

  3. Enterotoxins and emetic toxins production by Bacillus cereus and other species of Bacillus isolated from Soumbala and Bikalga, African alkaline fermentedfood condiments

    DEFF Research Database (Denmark)

    Ouoba, Labia Irene I.; Thorsen, Line; Varnam, Alan H.

    2008-01-01

    -hemolytic enterotoxin (NheA, NheB, NheC) and EM1 specific of emetic toxin producerswas also investigated using PCR with single pair and multiplex primers. Of 41 isolates, 29 Bacillus belonging to the species of B. cereus, Bacillus subtilis, Bacillus licheniformis and Bacillus pumilus showed haemolysis on blood agar......The ability of various species of Bacillus from fermented seeds of Parkia biglobosa known as African locust bean(Soumbala) and fermented seeds of Hibiscus sabdariffa (Bikalga) was investigated. The study included screening of the isolates by haemolysis on blood agar, detection of toxins in broth...... and during the fermentation of African locust bean using the Bacillus cereus Enterotoxin Reverse Passive Latex Agglutination test kit (BCETRPLA) and the Bacillus Diarrhoeal Enterotoxin Visual Immunoassay (BDEVIA). Detection of genes encoding´cytotoxin K (CytK), haemolysin BL (Hbl A, Hbl C, Hbl D), non...

  4. Physicochemical Evaluation of Seeds and Oil of Nontraditional Oil Seeds

    Directory of Open Access Journals (Sweden)

    Adam Ismail Ahmed

    2015-08-01

    Full Text Available The present work was conducted in the Laboratory of Biochemistry and Food science department, Faculty of Natural Resources and Environmental Studies, University of Kordofan, in order to evaluate some nontraditional oil seeds these are i.e. Marula (Sclerocarya birrea, Roselle (Hibiscus sabdariffa L. seeds and Christ’s thorn (Zizyphus spina-christi seeds. The seeds of the roselle and Christ’s thorn fruits were procured from Elobeid local market, North Kordofan State, while marula fruits were obtained from Elnuhod, West Kordofan State. The proximate composition of the seeds, cake and christ’s thorn pulp was done. Some chemical and physical properties were performed for the extracted oil. The results revealed that proximate composition of the seeds and cake differ statistically among the studied materials. Significant differences were observed among the oil extracted from these species; moreover, these oils differ significantly in color and viscosity only.

  5. Coping With Hypertension Using Safer Herbal Medicine ? A Therapeutic Review

    Directory of Open Access Journals (Sweden)

    Lakshmi.T

    2011-06-01

    Full Text Available Hypertension (HT is a major modifiable risk factor for cardiovascular disease, and if treated can significantly reduce cardiovascular disease risk. Herbal medicines are in great demand in the developed as well as developing countries for primary healthcare because of their wide biological and medicinal activities, higher safety margins and lesser cost. our article reviews the efficacy of some of valuable herbs like Allium sativum, Centella asiatica, Ginkgo biloba, Crataegus oxycantha, Crataegus monogyna, Passiflora Edulis, Hibiscus sabdariffa, Elaeocarpus ganitrus, Hypericum perforatum, Achillea millefolium. They have a history of human use and their Anti hypertensive properties have been evaluated preclinically and clinically. The present literature emphasizes on causes for hypertension, its signs, symptoms, preventive measures as well as its safer options of treatments. Available data suggests that the extracts of most of these herbs or compounds derived from them may provide a safe and effective adjunctive therapeutic approach for the treatment of hypertension

  6. Use of antioxidants substances to protect the hydrocolloids carrageenan, agaran and alginates used in food industry when exposed to radiation; Uso de substancias antioxidantes na resposta a radiacao dos hidrocoloides carragenanas, agaranas e alginatos utilizados na industria alimenticia

    Energy Technology Data Exchange (ETDEWEB)

    Aliste, Antonio Joao

    2006-07-01

    Carrageenan, agaran e alginates are hydrocolloids largely employed in every kind of food products as stabilizing agent and viscosity builder. The human body does not absorb them, so they do not introduce extra calories in the diet. Irradiation is presented as an important alternative method in food preservation because do not induce temperature increase being of good efficiency in cold food ingredients decontamination. In this work aqueous solutions of carrageenan, agar e sodium alginate were gamma irradiated (0-10 kGy) in presence of ascorbic acid, roselle (Hibiscus sabdariffa L.) extract and soy isoflavone. Edible polysaccharide solutions showed to be suitable systems for the evaluation of ionizing radiation effects as they presented a singular radiosensitivity through viscosity changes. The results obtained showed that in general the antioxidants employed had a radioprotective action that can be of importance in the future commercial applications of food irradiation. (author)

  7. Microbial contaminants of cultured Hibiscus cannabinus and Telfaria ...

    African Journals Online (AJOL)

    Administrator

    ISSN 1684–5315 © 2004 Academic Journals ... Nine microbial contaminants comprising of five bacteria and four fungi ... Key words: Micro-propagation, plant tissue culture, bacterial and fungal isolates, contamination. .... Surface sterilization of.

  8. Antioxidant activity of phenolic extracts from kenaf (Hibiscus cannabinus seedcake.

    Directory of Open Access Journals (Sweden)

    Ismail, N.

    2012-06-01

    Full Text Available The antioxidant activities of kenaf seedcake methanolic extract (CME and fractions obtained from it using ethyl acetate (EAF, hexane (HF and water (WF were investigated. Total phenolic contents were 64.5, 36.1, 31.3 and 14.6 mg gallic acid equivalent/g dry weight in EAF, CME, WF, and HF, respectively. Kenaf seedcake extract/fractions showed inhibitory activity of β-carotene bleaching and corn oil oxidation. Also, the extract/fractions were scavenged for the 1,1-diphenyl-2-picrylhydrazyl radical. The EAF extract showed the highest DPPH radical scavenging activity followed by the CME, WF and HF extracts. Therefore, the rich phenolic fractions of kenaf seedcake may represent a potential source of natural antioxidants. The predominant phenolic compounds identified by HPLC-DAD in CME and HF extracts were gallic, (+-catechin, chlorogenic, hydroxybenzoic, syringic, and vanillin.Se ha investigado la actividad antioxidante de extractos metanólicos procedentes de tortas de semillas de kenaf (CME y de las fracciones obtenidas usando acetato de etilo (EAF, hexano (HF y agua (WF. Los contenidos fenólicos totales fueron 64,5, 36,1, 31,3 y 14,6 mg de equivalente de ácido gálico/g de peso seco, en EAF, CME, WF, y HF, respectivamente. Los extractos/fracciones de semillas de kenaf mostraron actividad inhibitoria de blanqueo del β-caroteno y oxidación del aceite de maíz. Además, los extractos/fracciones fueron captadores de radicales del 1,1-difenil-2-picrilhidrazil. El extracto EAF mostró la mayor actividad captadora de radicales de DPPH seguido por los extractos de CME, WF y HF. Por lo tanto, las fracciones ricas en fenoles de las tortas de semilla de kenaf pueden representar una fuente potencial de antioxidantes naturales. Los compuestos fenólicos predominantes identificados mediante HPLC-DAD en extractos de CME y HF fueron gálico, (+-catequina, ácido clorogénico, hidroxibenzoico, siríngico, y vainillina.

  9. VEGETATIVE COMPATIBILITY GROUPS OF FUSARIUM OXYSPORUM, THE CAUSAL ORGANISM OF VASCULAR WILT ON ROSELLE IN MALAYSIA

    Directory of Open Access Journals (Sweden)

    K.H. Ooi

    1999-01-01

    Full Text Available Forty strains of Fusarium oxysporvm isolated from roselle (Hibiscus sabdariffa var. sabdariffa showing vascular wilt symptoms in three states (Terengganu, Penang and Ipoh in the northern Malaysian Peninsula were used to investigate the vegetative co mpatibility. Nitrate-nonutilizing (nil mutants were recovered from all the strains tested and subsequently used to study vegetative compatibility groups (VCG within the population by nit mutants pairings on minimal medium. Thirteen VCGs were found and none were vegetatively compatible with those of other formae speciales (f. spp. such as asparagi and cubense, and non-pathogenic strains from paddy and oil palm. The results indicate that there is substantial genetic diversity in F. oxysporum that causes vascular wilt disease on roselle as reflected by multiple VCGs, but the distribution of strains into the VCGs is not even as there are 26 representatives in VCG-1001M, two in VCG-1003M and VCG-1013M and only one in the other VCGs. This study may provide new insight into the establishment of a new forma specialis off. oxysporum.

  10. ПЕРСПЕКТИВЫ ИСПОЛЬЗОВАНИЯ ЭКСТРАКТОВ ИЗ HIBISCUS ROSA-SINENSIS И ЛЕКАРСТВЕННЫХ РАСТЕНИЙ ДЛЯ ПРОИЗВОДСТВА НАПИТКОВ

    Directory of Open Access Journals (Sweden)

    А. В. Георгиева

    2016-06-01

    Full Text Available С целью обогащения напитков биологически активными веществами из гибискуса китайского(Hibiscus rosa-sinensis и лекарственных растений, проведены исследования их водных экстрактов.На основе полученных водных экстрактов разработаны композиции для обогащения напитков функционального назначения. Исследован химический состав Hibiscus rosa-sinensis и антиоксидантная активность разработанных композиций. Определены содержание и степень этерификации пектина в цветах Hibiscus rosa-sinensis и в лекарственных растениях. В составе пектиновых веществ исследуемого сырья содержится большое количество свободных карбоксильных групп, что обуславливает их способность к связыванию ионов тяжелых металлов и выведению их из организма человека. Исследова количественный и качественный состав свободных аминокислот плодах Rosa canina и в цветах Hibiscus rosa-sinensis.

  11. Screening des microorganismes à potentialités fermentaires pour le manioc

    Directory of Open Access Journals (Sweden)

    Essia Ngang, JJ.

    2005-01-01

    Full Text Available Screening for Properties of some Strains of Lactobacilli with Potentials Applications as Starter Culture for cassava fermentation. The present study is focussed on the nutritional importance of consumed leafy vegetables in Bini- Dang situated in the Adamawa savannah in Ngaoundere (Cameroon. Firstly, inquiries are held on some ethnonutritional data on the usage and consumption of leafy vegetables. Secondly, physicochemical analyses have been carried out on some nutrient and antinutrient contents of eight most consumed leafy vegetables recorded. They are: Thalinum triangulare, Momordica charantia, Moringa oleifera, Hibiscus cannabinus, H. sabdariffa, Cerathotheca sesamoïdes, Corchorus olithorus and Vigna unguiculata. Results obtained show that: the locality of Bini- Dang has an ethnic diversity with about 103 families composed of 19 ethnic groups, giving a total of 621 persons (adults and children interrogated. Twentyfour leafy vegetables of different species are consumed. Among these vegetables, 66.66% are cultivated, 33.33% are picked from nature. The results based on statistical analyses of nutrients and antinutrients (P≤ 0.05 showed that these leafy vegetables were high in levels of carbohydrates 43.35 0.03% (C. olithorus to 50.16 0.01% (H. sabdariffa dry weight (DW and average levels of proteins ranged from 18.39 0.01% DW (H. sabdariffa to 26.48 0.01% DW (V. unguiculata. Total ashes are also important with contents between 8% (M. oleifera and 14% (T. triangulare. Iron, the most representative mineral was found to be between 3.81 0.12% for M. oleifera and 10.13 0.36% for M. charantia. It was the only mineral found in high quantity. Vitamin C was also found to be high in V. unguiculata and T. triangulare more than 4% and the levels of pigments and antinutrents were low in all the vegetables analysed. The fibre levels were between 10.63 0.18% DW for M. charantia and 15.40 0.13% DW for H. sabdariffa. These leafy vegetables studied were found

  12. Effects of Organic and Chemical Fertilizers on some Quantitative Traits and Anthocyanin of Roselle under Zabol conditions

    Directory of Open Access Journals (Sweden)

    rahman Ebrahimzadeh abdashti

    2017-02-01

    Full Text Available Introduction: Roselle (Hibiscus sabdariffa L. belongs to the Malvaceae family, and is an annual or biennial plant that cultivated in tropical and subtropical regions for its stem fibers, eatable calyces, leaves and grains. Roselle is resistant to relatively high temperatures throughout the growing and fruiting times. Continuous use of chemical fertilizers destroys the soil ecological balance, reduces soil fertility and groundwater pollution is caused. In contrast, organic fertilizers are very safe for human health and the environment. It is made by recycling organic material as plant and animals waste, and food scraps in a controlled process. Of the organic fertilizers can be noted to compost, vermicompost, cattle manure andhumic acid. The study was carried out in order to sustainable agriculture. The aim of this study was to investigate the effects of organic and chemical fertilizers on some quantitative traits and anthocyanin of roselle. Materials and Methods: The experiment was performed in complete randomized block design with ten treatments and four replications in research field of agricultural faculty, university of zabol. The treatments included T1: control, T2: NPK in a ratio of 2:1:1 (300 kg ha-1, T3: cattle manure (20 t ha-1, T4: compost (10 t ha-1, T5: vermicompost (5 t ha-1, T6: humic acid foliar in a ratio of 1.5 per thousands, T7: 50% of T2 and T6 , T8: 50% of T3 and T6, T9: 50% of T4 and T6 and T10: 50% of the T5 and T6. Five plants were chosen and an average of five plants was calculated as the single plant for measuring of variables that included the height, number of branches, stem diameter, shoot fresh weight, number of fruits per plant, fruit weight, number of seeds per plant, fresh and dry weights of sepals, inflorescence length, length and diameter of fruit. Method of Wagner has been used for the anthocyanin measurement. Results and Discussion: Results of this research showed that the application of different fertilizers

  13. Environ: E00348 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00348 Hibiscus syriacus bark Crude drug Hibiscus syriacus [TAX:106335] Malvaceae (mallow family) Hibis...cus syriacus bark Crude drugs [BR:br08305] Dicot plants: rosids Malvaceae (mallow family) E00348 Hibiscus syriacus bark ...

  14. Effect of ethnomedicinal plants used in folklore medicine in Jordan as antibiotic resistant inhibitors on Escherichia coli

    Directory of Open Access Journals (Sweden)

    Aburjai Talal A

    2010-02-01

    Full Text Available Abstract Background Escherichia coli occurs naturally in the human gut; however, certain strains that can cause infections, are becoming resistant to antibiotics. Multidrug-resistant E. coli that produce extended-spectrum β lactamases (ESBLs, such as the CTX-M enzymes, have emerged within the community setting as an important cause of urinary tract infections (UTIs and bloodstream infections may be associated with these community-onsets. This is the first report testing the antibiotic resistance-modifying activity of nineteen Jordanian plants against multidrug-resistant E. coli. Methods The susceptibility of bacterial isolates to antibiotics was tested by determining their minimum inhibitory concentrations (MICs using a broth microdilution method. Nineteen Jordanian plant extracts (Capparis spinosa L., Artemisia herba-alba Asso, Echinops polyceras Boiss., Gundelia tournefortii L, Varthemia iphionoides Boiss. & Blanche, Eruca sativa Mill., Euphorbia macroclada L., Hypericum trequetrifolium Turra, Achillea santolina L., Mentha longifolia Host, Origanum syriacum L., Phlomis brachydo(Boiss. Zohary, Teucrium polium L., Anagyris foetida L., Trigonella foenum-graecum L., Thea sinensis L., Hibiscus sabdariffa L., Lepidium sativum L., Pimpinella anisum L. were combined with antibiotics, from different classes, and the inhibitory effect of the combinations was estimated. Results Methanolic extracts of the plant materials enhanced the inhibitory effects of chloramphenicol, neomycin, doxycycline, cephalexin and nalidixic acid against both the standard strain and to a lesser extent the resistant strain of E. coli. Two edible plant extracts (Gundelia tournefortii L. and Pimpinella anisum L. generally enhanced activity against resistant strain. Some of the plant extracts like Origanum syriacum L.(Labiateae, Trigonella foenum- graecum L.(Leguminosae, Euphorbia macroclada (Euphorbiaceae and Hibiscus sabdariffa (Malvaceae did not enhance the activity of

  15. Traditional leafy vegetables in Senegal: diversity and medicinal uses.

    Science.gov (United States)

    Mathieu, Gueye; Meissa, Diouf

    2007-06-10

    Six administrative regions of Senegal were investigated. Forty species of vegetable leaves which are traditionally consumed in Senegal have been inventoried. All species are members of twenty-one families the most numerous of which are Amaranthaceae Juss., Malvaceae Juss., Moraceae Link., the Papilionaceae Giseke and Tiliaceae Juss. The species are subdivided into three groups: cultivated leafy vegetables, plants gathered annually, perennial sub-ligneous and ligneous species. The gathered species represent 67.5% of the inventory, 40.7% of which is ligneous. Cultivated species account for 32.5% of the inventory. The species are consumed for their medicinal properties, nutritive value and eating habits linked to specific ethnic traditions. During the drought years, with the scarcity of main food (millet, mays) consumption of leafy vegetables is high. All species reported except Sesuvium portulacastrum L. are consumed like vegetable herbs. The species of Hibiscus are eaten in spinach and condiment form while Sesuvium portulacastrum L is cooked in salad. Of the forty species examined, eleven are widely consumed. Within the entire study area, Hibiscus sabdariffa predominates among species consumed, followed by Moringa oleifera Lam. and Senna obtusifolia Link. A high consumption level of some species like amarante, Corchorus tridens L., Corchorus aestuans L., Leptadenia hastata Decne. and Vigna unguiculata (L.) Walp is confined to certain areas. In addition to their consumption as vegetables, the medicinal uses of 57.5% of these is of primary importance. The most commonly exploited parts are, respectively, leaf (40%), roots (20%), and bark (13.3%). Among the numerous pathologies treated, abscess, constipation, and rheumatism are predominant followed by aphrodisiac uses. The Amaranthus spp. L., Leptadenia hastata Decne., Senna obtusifolia Link., Adansonia digitata L. and Tamarindus indica L. are species with multiple medicinal uses.

  16. Natural red dyes extraction on roselle petals

    Science.gov (United States)

    Inggrid, H. M.; Jaka; Santoso, H.

    2016-11-01

    Roselle (Hibiscus sabdariffa L.) has a high quantity of anthocyanin pigment and is a good colorant. The anthocyanin pigment can be used as a natural colorant and antioxidant. An antioxidant is an organic compound that has the ability to inhibit free radical reactions in the human body. The objective of this research is to study the effect of pH and temperature on total anthocyanin and antioxidant activity in roselle extract, and to evaluate the effect of temperature and sunlight on the stability of the red color from roselle. Dried roselle petals were extracted with solid liquid extraction method using water as solvent. The variables in this study are temperature (5°C, 30°C, and 55°C) and pH (2, 7, and 12). Total anthocyanin was analysed using the pH differential method. The antioxidant activities were determined using the DPPH method. The highest total anthocyanin in the roselle petals was 80.4 mg/L at a temperature of 5°C and pH 2. The highest antioxidant activity and yield content in the roselle were 90.4% and 71.6 % respectively, obtained at 55°C and pH 2.

  17. In vitro anti-hepatoma activity of fifteen natural medicines from Canada.

    Science.gov (United States)

    Lin, Liang-Tzung; Liu, Li-Teh; Chiang, Lien-Chai; Lin, Chun-Ching

    2002-08-01

    Fifteen crude drugs, Stellaria media Cyrill. (Caryophyllaceae), Calendula officinalis L. (Compositae), Achillea millefolium L. (Compositae), Verbascum thapsus L. (Scrophulariaceae), Plantago major L. (Plantaginaceae), Borago officinalis L. (Boraginaceae), Satureja hortensis L. (Labiatae), Coptis groenlandica Salisb. (Ranunculaceae), Cassia angustifolia Vahl. (Leguminosae), Origanum majorana L. (Labiatae), Centella asiatica L. (Umbelliferae), Caulophyllum thalictroides Mich. (Berberidaceae), Picea rubens Sargent. (Pinaceae), Rhamnus purshiana D.C. (Rhamnaceae) and Hibiscus sabdariffa L. (Malvaceae), which have been used as folk medicine in Canada, were evaluated for their anti-hepatoma activity on five human liver-cancer cell lines, i.e. HepG2/C3A, SK-HEP-1, HA22T/VGH, Hep3B and PLC/PRF/5. The samples were examined by in vitro evaluation for their cytotoxicity. The results showed that the effects of crude drugs on hepatitis B virus genome-containing cell lines were different from those against non hepatitis B virus genome-containing cell lines. C. groenlandica was observed to be the most effective against the growth of all five cell lines and its chemotherapeutic values will be of interest for further studies.

  18. Medicinal Plants Traditionally Used for Treatment of Obesity and Diabetes Mellitus - Screening for Pancreatic Lipase and α-Amylase Inhibition.

    Science.gov (United States)

    Buchholz, Tina; Melzig, Matthias F

    2016-02-01

    In order to find new pancreatic lipase (PL) and α-amylase inhibitors from natural sources for the treatment of obesity and related diseases as diabetes mellitus II, 23 medicinal plants with weight-reducing, serum glucose-reducing or related potential were investigated. Methanolic and water extracts of the plants were evaluated by using two in vitro test systems. Our findings have shown that the methanolic extract of Hibiscus sabdariffa L. (Malvaceae) showed high inhibitory activities to PL (IC50 : 35.8 ± 0.8 µg/mL) and α-amylase (IC50 : 29.3 ± 0.5 µg/mL). Furthermore, the methanolic extract of Tamarindus indica L. (Leguminosae) showed a high anti-lipase (IC50 : 152.0 ± 7.0 µg/mL) and the aqueous extract a high anti-amylase (IC50 : 139.4 ± 9.0 µg/mL) activity. This work provides a priority list of interesting plants for further study with respect to the treatment of obesity and associated diseases.

  19. Control of Root Rot and Wilt Diseases of Roselle under Field Conditions

    Science.gov (United States)

    Hassan, Naglaa; Elsharkawy, Mohsen Mohamed; Shimizu, Masafumi

    2014-01-01

    Roselle (Hibiscus sabdariffa L.) is one of the most important medicinal crops in many parts of the world. In this study, the effects of microelements, antioxidants, and bioagents on Fusarium oxysporum, F. solani, and Macrophomina phaseolina, the causal pathogens of root rot and wilt diseases in roselle, were examined under field conditions. Preliminary studies were carried out in vitro in order to select the most effective members to be used in field control trials. Our results showed that microelements (copper and manganese), antioxidants (salicylic acid, ascorbic acid, and EDTA), a fungicide (Dithane M45) and biological control agents (Trichoderma harzianum and Bacillus subtilis) were significantly reduced the linear growth of the causal pathogens. Additionally, application of the previous microelements, antioxidants, a fungicide and biological control agents significantly reduced disease incidence of root rot and wilt diseases under field conditions. Copper, salicylic acid, and T. harzianum showed the best results in this respect. In conclusion, microelements, antioxidants, and biocontrol agents could be used as alternative strategies to fungicides for controlling root rot and wilt diseases in roselle. PMID:25606010

  20. REVITALISASI KONSERVASI TUMBUHAN OBAT KELUARGA (TOGA GUNA MENINGKATKAN KESEHATAN DAN EKONOMI KELUARGA MANDIRI DI DESA CONTOH LINGKAR KAMPUS IPB DARMAGA BOGOR

    Directory of Open Access Journals (Sweden)

    Agus Hikmat

    2011-08-01

    Full Text Available Medicinal plants and traditional medicine for along ago are important role in the health care, stamina maintain, and treat diseases. Therefore medicinal plants and traditional medicines have strong root in the part of community up to now. Research on revitalization of family medicinal plant (TOGA conservation done at Kampong Pabuaran (Cibanteng village, and Kampong Gunung Leutik ( Benteng village, results indicated that research locations have completely medicinal plants diversity for medicine all diseases of village communities mentioned. Number of medicinal plants found at Gunung Leutik and Pabuaran Sawah Kampong (Cibanteng and Benteng Villages were 237 spesies, and 95 spesies often used by respondents mentioned villages. Species number of medicinal plants have potential to expand based on use value of these species treat main diseases of community villages Gunung Leutik and Pabuaran Sawah Villages (Benteng and Cibanteng were 15 spesies, such as: sambiloto (Andrographis paniculata (Burm.F Ness., meniran (Phyllanthus niruri L., takokak (Solanum torvum L., pegagan (Centella asiatica (L. Urban., temulawak (Curcuma xanthorrhiza Roxb., jahe (Zingiber officinale-purpurea Rosc., jeruk nipis (Citrus aurantifolia (Christm & Panz Swingle, binahong (Anredera cordifolia, mahkota dewa (Phaleria macrocarpus (Sheff. Boerl., rosella (Hibiscus sabdariffa, pule pandak (Rauvolfia serpentine (L. Benth. ex. Kurz., sangitan (Sambucus javanica Reinw., sirih (Piper betle L., brotowali (Tinospora crispa, and kenikir (Cosmos caudatus

  1. Photostability of low cost dye-sensitized solar cells based on natural and synthetic dyes

    Science.gov (United States)

    Abdou, E. M.; Hafez, H. S.; Bakir, E.; Abdel-Mottaleb, M. S. A.

    2013-11-01

    This paper deals with the use of some natural pigments as well as synthetic dyes to act as sensitizers in dye-sensitized solar cells (DSSCs). Anthocyanin dye extracted from rosella (Hibiscus sabdariffa L.) flowers, the commercially available textile dye Remazole Red RB-133 (RR) and merocyanin-like dye based on 7-methyl coumarin are tested. The photostability of the three dyes is investigated under UV-Vis light exposure. The results show a relatively high stability of the three dyes. Moreover, the photostability of the solid dyes is studied over the TiO2 film electrodes. A very low decolorization rates are recorded as; rate constants k = 1.6, 2.1 and 1.9 × 10-3 min-1 for anthocyanin, RR and coumarin dyes, respectively. The stability results favor selecting anthocyanin as a promising sensitizer candidate in DSSCs based on natural products. Dyes-sensitized solar cells are fabricated and their conversion efficiency (η) is 0.27%, 0.14% and 0.001% for the anthocyanin, RR and coumarin dyes, respectively. Moreover, stability tests of the sealed cells based on anthocyanin and RR dyes are done under continuous light exposure of 100 mW cm-2, reveals highly stable DSSCs.

  2. Control of Root Rot and Wilt Diseases of Roselle under Field Conditions.

    Science.gov (United States)

    Hassan, Naglaa; Elsharkawy, Mohsen Mohamed; Shimizu, Masafumi; Hyakumachi, Mitsuro

    2014-12-01

    Roselle (Hibiscus sabdariffa L.) is one of the most important medicinal crops in many parts of the world. In this study, the effects of microelements, antioxidants, and bioagents on Fusarium oxysporum, F. solani, and Macrophomina phaseolina, the causal pathogens of root rot and wilt diseases in roselle, were examined under field conditions. Preliminary studies were carried out in vitro in order to select the most effective members to be used in field control trials. Our results showed that microelements (copper and manganese), antioxidants (salicylic acid, ascorbic acid, and EDTA), a fungicide (Dithane M45) and biological control agents (Trichoderma harzianum and Bacillus subtilis) were significantly reduced the linear growth of the causal pathogens. Additionally, application of the previous microelements, antioxidants, a fungicide and biological control agents significantly reduced disease incidence of root rot and wilt diseases under field conditions. Copper, salicylic acid, and T. harzianum showed the best results in this respect. In conclusion, microelements, antioxidants, and biocontrol agents could be used as alternative strategies to fungicides for controlling root rot and wilt diseases in roselle.

  3. Physico Chemical Properties and Antioxidant Activity of Roselle Seed Extracts

    Directory of Open Access Journals (Sweden)

    Abdoulaye Idrissa Cissouma

    2013-11-01

    Full Text Available The aim of this research was to extract phenolic compounds from defatted Roselle (Hibiscus sabdariffaL. seed and assess their antioxidant potential. Water, ethanol (30%, methanol (30% and acetone (30% were used as solvent for extraction. The proximate composition, total phenolic content and extraction yield were analyzed. Antioxidant efficacies of Roselle seed extract were tested by using 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH, hydroxyl, 2, 2’-azinobis-3-ethylbenzothiaz oline-6-sulfonic acid (ABTS radicals scavenging capacities and reducing power analysis. Roselle seeds were found to be rich in protein (27.745%, carbohydrates (40.45% and oil (20.83%. The total phenolic content ranged from 1.66±0.03 to 1.99±0.01 (GAE mg/g using water and 30% acetone respectively. The highest inhibitory capacity on DPPH and ABTS radicals was observed in 30% acetone extract and was at 3 mg/mL for DPPH and 6 mg/mL for ABTS respectively. Ethanol extract showed the highest hydroxyl radical scavenging ability value of 66.36 at 20 mg/mL, followed by methanol (57.27, acetone (56.36 and water (30. The reducing potential of the different extracts was concentration dependent and increased with increase in concentration. These results indicate that substantial antioxidant activity can be obtained from Roselle seed phenolic compounds extract by using 30% acetone.

  4. Quality and Antioxidant Activity of Yogurt Supplemented with Roselle during Cold Storage

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    El Latifa Sri Suharto

    2016-08-01

    Full Text Available The purpose of this study was to determine the quality (physical, chemical, microbiological characteristics, total phenolic content, and antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH inhibition assay of probiotic yogurt supplemented with roselle flower extract (Hibiscus sabdariffa L during cold storage. The experiment used treatment for types of yogurt as follows: cow’s milk probiotic yogurt + roselle, goat’s milk probiotic yogurt + roselle, cow’s milk yogurt, and goat’s milk yogurt. The yogurt was stored in cold storage and evaluated the quality and antioxidant activity variables on days 0, 3, 6, 9, 12, and 15th. The results showed that there were interaction (P<0.05 between types of yogurt and storage time on pH value and total lactic acid bacteria (LAB, but no interaction effect on viscosity. The types of yogurt significantly affected (P<0.05 aw, total titrable acid (TTA, total phenolic content, and antioxidant activity. Cow’s milk probiotic yogurt + roselle and goat’s milk probiotic yogurt + roselle were the best yogurt that contributed to a good quality and high antioxidant activity up to 15 d at cold storage.

  5. Photostability of low cost dye-sensitized solar cells based on natural and synthetic dyes.

    Science.gov (United States)

    Abdou, E M; Hafez, H S; Bakir, E; Abdel-Mottaleb, M S A

    2013-11-01

    This paper deals with the use of some natural pigments as well as synthetic dyes to act as sensitizers in dye-sensitized solar cells (DSSCs). Anthocyanin dye extracted from rosella (Hibiscus sabdariffa L.) flowers, the commercially available textile dye Remazole Red RB-133 (RR) and merocyanin-like dye based on 7-methyl coumarin are tested. The photostability of the three dyes is investigated under UV-Vis light exposure. The results show a relatively high stability of the three dyes. Moreover, the photostability of the solid dyes is studied over the TiO2 film electrodes. A very low decolorization rates are recorded as; rate constants k=1.6, 2.1 and 1.9×10(-3)min(-1) for anthocyanin, RR and coumarin dyes, respectively. The stability results favor selecting anthocyanin as a promising sensitizer candidate in DSSCs based on natural products. Dyes-sensitized solar cells are fabricated and their conversion efficiency (η) is 0.27%, 0.14% and 0.001% for the anthocyanin, RR and coumarin dyes, respectively. Moreover, stability tests of the sealed cells based on anthocyanin and RR dyes are done under continuous light exposure of 100mWcm(-2), reveals highly stable DSSCs.

  6. Survey on medicinal plants and spices used in Beni-Sueif, Upper Egypt

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    Mohamed Abdelhalim A

    2011-06-01

    Full Text Available Abstract Background This study was conducted to identify medicinal plants and spices used for medicine by the community of Beni-Sueif, Upper Egypt. Methods Ethnobotanical data from local people was collected using direct interviews and a semi-structured questionnaire. Results Forty-eight plant species belonging to twenty-seven families and forty-seven genera were encountered during the study. Their botanical and vernacular names, plant parts used and medicinal uses are given. Results of the study were analyzed using two quantitative tools. The factor informant consensus indicated the agreement in the use of plants and the fidelity level indicated the ratio between the number of informants who independently suggested the use of a species for the same major purpose and the total number of informants who mentioned the plant for any use. The results of the factor informant consensus showed that the cardiovascular category has the greatest agreement, followed by the immunological, gastrointestinal and respiratory categories. The most important species according to their fidelity are: Hibiscus sabdariffa L. for the cardiovascular category; Trigonella foenum-graecum L. for the immunological category; Mentha piperita L. for the gastrointestinal category and Pimpinella anisum L. for the respiratory category. Conclusions Medicinal plants are still used for treatment in Beni-Sueif community despite the availability of prescribed medications. Documentation of this ethnomedicinal knowledge is important. Evaluation of pharmacological activity for the promising medicinal plants is suggested.

  7. Toxicity and mutagenic activity of some selected Nigerian plants.

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    Sowemimo, A A; Fakoya, F A; Awopetu, I; Omobuwajo, O R; Adesanya, S A

    2007-09-25

    The toxicity and mutagenic potential of most African plants implicated in the management of cancer have not been investigated. The ethanolic extracts of selected Nigerian plants were subsequently studied using the brine shrimp lethality tests, inhibition of telomerase activity and induction of chromosomal aberrations in vivo in rat lymphocytes. Morinda lucida root bark, Nymphaea lotus whole plant and Garcinia kola root were active in the three test systems. Bryophyllum calycinum whole plant, Annona senegalensis root, Hymenocardia acida stem bark, Erythrophleum suaveolens leaves and Spondiathus preussii stem bark were toxic to brine shrimps and caused chromosomal damage in rat lymphocytes. Ficus exasperata leaves, Chrysophyllum albidum root bark and Hibiscus sabdariffa leaves were non-toxic to all the three test systems. Chenopodium ambrosioides whole plant was non-toxic to brine shrimps and rat lymphocyte chromosomes but showed inhibition in the conventional telomerase assay indicating a possible selectivity for human chromosomes. The result justified the use of the first eight plants and Chenopodium ambrosioides in the management of cancer in south west Nigeria although they appear to be non-selective and their mode of action may be different from plant to plant. All these plants except Chenopodium ambrosioides are also mutagenic and cytotoxic.

  8. KADAR HORMON AUKSIN PADA TANAMAN KENAF (Hibiscus cannabinus L.) BERCABANG DAN TIDAK BERCABANG

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    Yunin Hidayati

    2009-01-01

    Kenaf is one of fiber producing plants. According to the existence of branches, kenaf is classified into two group, consisting of: kenaf with branch and with no branch.Morphology observation, consist of the height of plant, the number of node, the number of branch and the length of branch was observed. The morphology observation showed that there were differences morphological characters, including of the height of plant, the number of node, the length of branch, and the number of node more b...

  9. KADAR HORMON AUKSIN PADA TANAMAN KENAF (Hibiscus cannabinus L. BERCABANG DAN TIDAK BERCABANG

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    Yunin Hidayati

    2009-09-01

    Full Text Available Kenaf is one of fiber producing plants. According to the existence of branches, kenaf is classified into two group, consisting of: kenaf with branch and with no branch.Morphology observation, consist of the height of plant, the number of node, the number of branch and the length of branch was observed. The morphology observation showed that there were differences morphological characters, including of the height of plant, the number of node, the length of branch, and the number of node more branching kenaf, control non branching (KR11 and control branching (SM004.Non branching plants (KR11 have the highest habitus and smallest number of branch compare to other groups. Control branching (SM004 was the shortest habitus and greatest number of branch compare to other group. The highest of Non branching plants (KR11 230,6 ± 36,7 cm and the smallest control branching (SM004 116,3 ± 64,4 cm. The greatest number of branch found in controled branch SM004, with 5,6 ± 2,7 branch in every plant and the smallest is KR11 with 0,8 ± 0,8 branch in every plant.According to this research the length of plant influence the branch. The highest level of auxin were in the shoot tip and the lowest level were in root tip. The higher auxin will produce less branch, the lower auxin will produce more branch.

  10. PULPING PROPERTIES OF KRAFT PULP OF NIGERIAN-GROWN KENAF (HIBISCUS CANNABINUS L.

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    Abiodun Oluwafemi Oluwadare

    2011-02-01

    Full Text Available This study was centered on finding a locally sourced alternative to imported long-fibre pulp for Nigerian pulp and paper mills. Fibre characteristics, chemical composition, and paper properties of pulp handsheets at different levels of kappa number and freeness in the range of 10 oSR and 62 oSR were evaluated using air-dried bast fibre obtained from decorticated kenaf plants grown in southern guinea savanna near Jebba, Nigeria. Kenaf bast fibre compared well with softwood, with an average fibre length of 2.90 mm, a flexibility ratio of 57%, and a Runkel ratio of 0.76. Ash, lignin, and pentosan contents were 0.6%, 12.5%, and 10.6%, respectively, while the cellulose content was 55.5%. Under alkali charge of 15.0 and, sulphidity of 17.5 with constant temperature, cooking time, and liquor-to-fibre ratio of 4.5:1, the screen yield was between 48.8 to 52.8 % with kappa number 12.04 to 20.5. Unbleached pulpsheets at kappa number between 15 and18.5 and pulp freeness 55 oSR and bleached pulp freeness between 148 and 336 mLs had better quality paper in terms of overall pulpsheet strength properties.

  11. Kenaf (Hibiscus cannabinus) and cowpea (Vigna unguiculata) as sugarcane cover crops

    Science.gov (United States)

    A Louisiana sugarcane field is typically replanted every four years due to declining yields, and, although, it is a costly process, it is both necessary and an opportunity to maximize the financial return during the next four year cropping cycle. Fallow planting systems (FPS) during the fallow perio...

  12. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus

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    Lans Cheryl A

    2006-10-01

    Full Text Available Abstract Background This paper is based on ethnobotanical interviews conducted from 1996–2000 in Trinidad and Tobago with thirty male and female respondents. Methods A non-experimental validation was conducted on the plants used for urinary problems and diabetes mellitus: This is a preliminary step to establish that the plants used are safe or effective, to help direct clinical trials, and to inform Caribbean physicians of the plants' known properties to avoid counter-prescribing. Results The following plants are used to treat diabetes: Antigonon leptopus, Bidens alba, Bidens pilosa, Bixa orellana, Bontia daphnoides, Carica papaya, Catharanthus roseus, Cocos nucifera, Gomphrena globosa, Laportea aestuans, Momordica charantia, Morus alba, Phyllanthus urinaria and Spiranthes acaulis. Apium graviolens is used as a heart tonic and for low blood pressure. Bixa orellana, Bontia daphnoides, Cuscuta americana and Gomphrena globosa are used for jaundice. The following plants are used for hypertension: Aloe vera, Annona muricata, Artocarpus altilis, Bixa orellana, Bidens alba, Bidens pilosa, Bonta daphnoides, Carica papaya, Cecropia peltata, Citrus paradisi, Cola nitida, Crescentia cujete, Gomphrena globosa, Hibiscus sabdariffa, Kalanchoe pinnata, Morus alba, Nopalea cochinellifera, Ocimum campechianum, Passiflora quadrangularis, Persea americana and Tamarindus indicus. The plants used for kidney problems are Theobroma cacao, Chamaesyce hirta, Flemingia strobilifera, Peperomia rotundifolia, Petiveria alliacea, Nopalea cochinellifera, Apium graveolens, Cynodon dactylon, Eleusine indica, Gomphrena globosa, Pityrogramma calomelanos and Vetiveria zizanioides. Plants are also used for gall stones and for cooling. Conclusion Chamaesyce hirta, Cissus verticillata, Kalanchoe pinnata, Peperomia spp., Portulaca oleraceae, Scoparia dulcis, and Zea mays have sufficient evidence to support their traditional use for urinary problems, "cooling" and high cholesterol

  13. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus.

    Science.gov (United States)

    Lans, Cheryl A

    2006-10-13

    This paper is based on ethnobotanical interviews conducted from 1996-2000 in Trinidad and Tobago with thirty male and female respondents. A non-experimental validation was conducted on the plants used for urinary problems and diabetes mellitus: This is a preliminary step to establish that the plants used are safe or effective, to help direct clinical trials, and to inform Caribbean physicians of the plants' known properties to avoid counter-prescribing. The following plants are used to treat diabetes: Antigonon leptopus, Bidens alba, Bidens pilosa, Bixa orellana, Bontia daphnoides, Carica papaya, Catharanthus roseus, Cocos nucifera, Gomphrena globosa, Laportea aestuans, Momordica charantia, Morus alba, Phyllanthus urinaria and Spiranthes acaulis. Apium graviolens is used as a heart tonic and for low blood pressure. Bixa orellana, Bontia daphnoides, Cuscuta americana and Gomphrena globosa are used for jaundice. The following plants are used for hypertension: Aloe vera, Annona muricata, Artocarpus altilis, Bixa orellana, Bidens alba, Bidens pilosa, Bonta daphnoides, Carica papaya, Cecropia peltata, Citrus paradisi, Cola nitida, Crescentia cujete, Gomphrena globosa, Hibiscus sabdariffa, Kalanchoe pinnata, Morus alba, Nopalea cochinellifera, Ocimum campechianum, Passiflora quadrangularis, Persea americana and Tamarindus indicus. The plants used for kidney problems are Theobroma cacao, Chamaesyce hirta, Flemingia strobilifera, Peperomia rotundifolia, Petiveria alliacea, Nopalea cochinellifera, Apium graveolens, Cynodon dactylon, Eleusine indica, Gomphrena globosa, Pityrogramma calomelanos and Vetiveria zizanioides. Plants are also used for gall stones and for cooling. Chamaesyce hirta, Cissus verticillata, Kalanchoe pinnata, Peperomia spp., Portulaca oleraceae, Scoparia dulcis, and Zea mays have sufficient evidence to support their traditional use for urinary problems, "cooling" and high cholesterol. Eggplant extract as a hypocholesterolemic agent has some support but

  14. Insulin secretion enhancing activity of roselle calyx extract in normal and streptozotocin-induced diabetic rats

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    Eamruthai Wisetmuen

    2013-01-01

    Full Text Available Background and Objective: Our recent study revealed the antihyperglycemic activity of an ethanolic extract of roselle calyxes (Hibiscus sabdariffa in diabetic rats. The present study had, therefore, an objective to investigate the mechanism underlying this activity. Materials and Methods: Male Sprague Dawley rats were induced to be diabetes by intraperitoneal injection of 45 mg/kg streptozotocin (STZ. Normal rats as well as diabetic rats were administered with the ethanolic extract of H. sabdariffa calyxes (HS-EE at 0.1 and 1.0 g/kg/day, respectively, for 6 weeks. Then, blood glucose and insulin levels, at basal and glucose-stimulated secretions, were measured. The pancreas was dissected to examine histologically. Results: HS-EE 1.0 g/kg/day significantly decreased the blood glucose level by 38 ± 12% in diabetic rats but not in normal rats. In normal rats, treatment with 1.0 g/kg HS-EE increased the basal insulin level significantly as compared with control normal rats (1.28 ± 0.25 and 0.55 ± 0.05 ng/ml, respectively. Interestingly, diabetic rats treated with 1.0 g/kg HS-EE also showed a significant increase in basal insulin level as compared with the control diabetic rats (0.30 ± 0.05 and 0.15 ± 0.01 ng/ml, respectively. Concerning microscopic histological examination, HS-EE 1.0 g/kg significantly increased the number of islets of Langerhans in both normal rats (1.2 ± 0.1 and 2.0 ± 0.1 islet number/10 low-power fields (LPF for control and HS-EE treated group, respectively and diabetic rats (1.0 ± 0.3 and 3.9 ± 0.6 islet number/10 LPF for control and HS-EE treated group, respectively. Conclusion: The antidiabetic activity of HS-EE may be partially mediated via the stimulating effect on insulin secretion.

  15. Cambio de peso vivo de caprinos en agostadero y consumo voluntario del suplemento semilla de jamaica (hibiscus saddariffa (Change weight live of kids in tropical forest and voluntary intake of the supplement jamaica seed (hibiscus saddariffa

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    Olivares Pérez, Jaime: nick: olivaares

    2006-03-01

    Full Text Available Resumen El estudio se realizo en el 2004, en la época de estiaje en el trópico seco de Guerrero, México, el objetivo fue evaluar el cambio de peso en caprinos suplementados con semilla entera de Jamaica bajo pastoreo en agostadero. El experimento fue un diseño completamente al azar, se utilizaron 18 caprinos machos híbridos Pelibuey con Nubia, distribuidos en dos grupos, un testigo y un experimental a los que se les aplicaron los tratamientos T1= pastoreo en agostadero y T2= pastoreo en agostadero mas semilla entera de Jamaica a libre acceso por media hora previa al pastoreo. Las variables de estudio fueron el cambio de peso vivo y el consumo voluntario de semilla de Jamaica. A los datos que registro la variable cambio de peso vivo, se les aplico un análisis de varianza y la prueba de Tukey para comparación de medias entre grupos (Steel y Torrie, 1988. El grupo de animales experimentales presentaron en promedio, una mayor ganancia de peso total y diario por animal (P<0.05 con 1.44ª kg y 0.024ª kg respectivamente y la menor ganancia fue para el grupo de animales testigo con 0.27b kg y 0.004b kg respectivamente para ganancia de peso total y diario por animal. El consumo voluntario de semilla entera de jamaica fue en promedio de 0.116 kg MS por animal por día. Se concluye que la semilla de jamaica representa un recurso nutritivo empleado como suplemento para el ganado caprino bajo pastoreo en agostadero en época de estiaje en ambientes tropicales. The study one carries out in the 2004, in the low water time in the tropic of Guerrero, Mexico, the objective was to evaluate the change of weight in kids suplementation with whole seed of Jamaica under in tropical forest shepherding. The experiment was a complete randomized design, 18 male hybrid kids Pelibuey with Nubia, distributed in two groups, a witness and an experimental one to those that were applied the treatments T1 = shepherding in agostadero and T2 = shepherding in agostadero but whole seed of Jamaica to free access for previous half hour to the shepherding. The study variables were the change of weight I live and the voluntary intake of Jamaica seed. To the data that register the variable change of weight I live, they are applied a variance analysis and the test of Tukey for stockings of comparison among groups (Steel y Torrie, 1988. The group of experimental animals presented on the average, a higher gain of total weight and daily for animal (P < 0.05 with 1.44ª kg and 0.024ª kg respectively and smaller gain was for animals witness group with 0.27b kg and 0.004b kg for gain of total weight and daily for animal. The voluntary intake of whole seed of jamaica was in average of 0.116 kg DM for animal per day. You concludes that the jamaica seed represents a resource nutritious for the livestock caprino in shepherding in the tropic.

  16. Cambio de peso vivo de caprinos en agostadero y consumo voluntario del suplemento semilla de jamaica (hibiscus saddariffa - Change weight live of kids in tropical forest and voluntary intake of the supplement jamaica seed (hibiscus saddariffa

    Directory of Open Access Journals (Sweden)

    Olivares Pérez, Jaime: nick: olivaares

    2006-08-01

    Full Text Available El estudio se realizo en el 2004, en la época de estiaje en el trópico seco de Guerrero, México, el objetivo fue evaluar el cambio de peso en caprinos suplementados con semilla entera de Jamaica bajo pastoreo en agostadero. El experimento fue un diseño completamente al azar, se utilizaron 18 caprinos machos híbridos Pelibuey con Nubia, distribuidos en dos grupos, un testigo y un experimental a los que se les aplicaron los tratamientos T1= pastoreo en agostadero y T2= pastoreo en agostadero mas semilla entera de Jamaica a libre acceso por media hora previa al pastoreo. Las variables de estudio fueron el cambio de peso vivo y el consumo voluntario de semilla de Jamaica. A los datos que registro la variable cambio de peso vivo, se les aplico un análisis de varianza y la prueba de Tukey para comparación de medias entre grupos (Steel y Torrie, 1988. El grupo de animales experimentales presentaron en promedio, una mayor ganancia de peso total y diario por animal (P<0.05 con 1.44ª kg y 0.024ª kg respectivamente y la menor ganancia fue para el grupo de animales testigo con 0.27b kg y 0.004b kg respectivamente para ganancia de peso total y diario por animal. El consumo voluntario de semilla entera de jamaica fue en promedio de 0.116 kg MS por animal por día. Se concluye que la semilla de jamaica representa un recurso nutritivo empleado como suplemento para el ganado caprino bajo pastoreo en agostadero en época de estiaje en ambientes tropicales. The study one carries out in the 2004, in the low water time in the tropic of Guerrero, Mexico, the objective was to evaluate the change of weight in kids suplementation with whole seed of Jamaica under in tropical forest shepherding. The experiment was a complete randomized design, 18 male hybrid kids Pelibuey with Nubia, distributed in two groups, a witness and an experimental one to those that were applied the treatments T1 = shepherding in agostadero and T2 = shepherding in agostadero but whole seed of Jamaica to free access for previous half hour to the shepherding. The study variables were the change of weight I live and the voluntary intake of Jamaica seed. To the data that register the variable change of weight I live, they are applied a variance analysis and the test of Tukey for stockings of comparison among groups (Steel y Torrie, 1988. The group of experimental animals presented on the average, a higher gain of total weight and daily for animal (P < 0.05 with 1.44ª kg and 0.024ª kg respectively and smaller gain was for animals witness group with 0.27b kg and 0.004b kg for gain of total weight and daily for animal. The voluntary intake of whole seed of jamaica was in average of 0.116 kg DM for animal per day. You concludes that the jamaica seed represents a resource nutritious for the livestock caprino in shepherding in the tropic.

  17. Microbiological and chemical changes during the fermentation of African locust bean (Parkia biglobosa) to produce afitin, iru and sonru, three traditional condiments produced in Benin.

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    Azokpota, P; Hounhouigan, D J; Nago, M C

    2006-04-01

    African locust bean (Parkia biglobosa) was processed to produce afitin, iru and sonru, three different types of condiment from Benin. Whereas the fermentation of African locust bean to produce afitin is carried out for 24 h without using any additive, the fermentation for iru and sonru production takes place for 48 h with adding respectively "iku-iru" and "yanyanku" two traditional malvacene bean-based (Hibiscus sabdariffa) additives. The main microorganisms involved in the fermentation of the three condiments were Bacillus spp., although Staphylococcus spp. was found in lower number. The use of additive seems to enhance the initial Bacillus counts in iru and sonru, but also a slightly higher Staphylococcus count was observed. The number of staphylococci did not exceed 10(2) cfu/g and it was below the detection level at the end of the fermentation (24 h) in afitin; it reached a level of about 10(6) cfu/g at 12-18 h in iru and sonru followed by a decrease to about 5 x 10(4) cfu/g after 48 h of fermentation. The final pH and total basic volatile nitrogen content of iru and sonru are higher than those of afitin. Proximate composition changes are similar in both fermenting iru and sonru, but different in afitin, the latter showing a slight decrease of proteins of about 5% whereas for iru and sonru they increased to about 6%. Ash, crude fat and fibre contents also increased during the fermentation in the three products whereas total carbohydrates decreased significantly. Due to their long fermentation time, iru and sonru are very soft products as they are preferred by some consumers whereas afitin is preferred with cotyledons having less soft texture.

  18. Evaluation of Nutritive and Antioxidant Properties of Blanched Leafy Vegetables Consumed in Northern Côte d’Ivoire

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    Oulai Patricia D.

    2015-03-01

    Full Text Available The aim of this study was to evaluate the effect of steam blanching processing on the nutritive value and the antioxidant properties of five leafy vegetable species (Amaranthus hybridus, Andasonia digitata, Ceiba patendra, Hibiscus sabdariffa and Vigna unguiculata that are used for sauce preparation in Northern CÔte d'Ivoire. The selected leafy vegetables were subjected to blanching in pressure cooker for 15, 25 and 45 min and the physicochemical properties were determined using AOAC methods. The result of the study revealed that longer time of blanching (higher than 15 min caused negative impact by reducing nutritive value but positive impact by reducing anti-nutrients and increasing polyphenols. The registered losses (p0.05 at 15 min were as follow: ash (0.08-10.01%, proteins (0.36-12.03%, vitamin C (19.56-68.67%, carotenoids (18.91-55.48% oxalates (3.58-21.39% and phytates (10.51-68.02%. The average increase of polyphenols contents at 15 min of blanching was 1.61 to 30.72%. In addition, a slight increase (0.35-4.16% of fibres content was observed in the studied blanched leafy vegetables. Furthermore, after 15 min of blanching time the residual contents (p0.05 of minerals were: calcium (264.88-844.92 mg/100 g, magnesium (49.45-435.43 mg/100 g, potassium (675-1895.41 mg/100 g, iron (14.54-70.89 mg/100 g and zinc (9.48-36.46 mg/100 g. All these results suggest that the recommended time of domestic blanching must be less than 15 min for the studied leafy vegetables in order to contribute efficiently to the nutritional requirement and to the food security of Ivorian population

  19. Enterotoxins and emetic toxins production by Bacillus cereus and other species of Bacillus isolated from Soumbala and Bikalga, African alkaline fermented food condiments.

    Science.gov (United States)

    Ouoba, Labia Irene I; Thorsen, Line; Varnam, Alan H

    2008-06-10

    The ability of various species of Bacillus from fermented seeds of Parkia biglobosa known as African locust bean (Soumbala) and fermented seeds of Hibiscus sabdariffa (Bikalga) was investigated. The study included screening of the isolates by haemolysis on blood agar, detection of toxins in broth and during the fermentation of African locust bean using the Bacillus cereus Enterotoxin Reverse Passive Latex Agglutination test kit (BCET-RPLA) and the Bacillus Diarrhoeal Enterotoxin Visual Immunoassay (BDEVIA). Detection of genes encoding cytotoxin K (CytK), haemolysin BL (Hbl A, Hbl C, Hbl D), non-hemolytic enterotoxin (NheA, NheB, NheC) and EM1 specific of emetic toxin producers was also investigated using PCR with single pair and multiplex primers. Of 41 isolates, 29 Bacillus belonging to the species of B. cereus, Bacillus subtilis, Bacillus licheniformis and Bacillus pumilus showed haemolysis on blood agar. Using RPLA, enterotoxin production was detected for three isolates of B. cereus in broth and all B. cereus (9) in fermented seeds. Using BDEVIA, enterotoxin production was detected in broth as well as in fermented seeds for all B. cereus isolates. None of the isolates belonging to the other Bacillus species was able to produce enterotoxins either by RPLA or BDEVIA. Nhe genes were detected in all B. cereus while Hbl and CytK genes were detected respectively in five and six B. cereus strains. A weak presence of Hbl (A, D) and CytK genes was detected in two isolates of B. subtilis and one of B. licheniformis but results were inconsistent, especially for Hbl genes. The emetic specific gene fragment EM1 was not detected in any of the isolates studied.

  20. Herbal extracts in oral health care - A review of the current scenario and its future needs

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    Byalakere Rudraiah Chandra Shekar

    2015-01-01

    Full Text Available Background: Oral diseases are among the major public health problems and the commonest of chronic diseases that affect mankind. The application of natural products for the control of oral diseases is considered as an interesting alternative to synthetic antimicrobials due to their lower negative impact, and for the effort to overcome primary or secondary resistance to the drug during therapy. Objective: To review the current evidence on the antimicrobial efficacy of 10 plant extracts on dental caries and plaque microorganisms. Materials and Methods: A comprehensive literature search was made by one of the authors for 2 months in PubMed, PubMed Central, MEDLINE, LILACS/BBO, Cochrane database of systematic reviews, SCIENCE DIRECT, and Google scholar databases. The results from the relevant published literatures are discussed. Summary and Conclusion: The extracts of Azadirachta Indica, Ocimum sanctum, Murraya koenigii L., Acacia nilotica, Eucalyptus camaldulensis, Hibiscus sabdariffa, Mangifera indica, Psidium guajava, Rosa indica, and Aloe barbadensis Miller have all been found to inhibit certain dental caries and periodontal pathogens. The current evidence is on individual plant extracts against bacteria involved in either caries or periodontitis. "Herbal shotgun" or "synergistic multitarget effects" are the terms used for the strategy of combining different extracts. The research assessing the antimicrobial efficacy of a combination of these plant extracts against dental caries and periodontal pathogens is the need of the hour, and such research will aid in the development of a novel, innovative method that can simultaneously inhibit two of the most common dental diseases of mankind, besides slowing the development of drug resistance.

  1. Nutritive and Antioxidant properties of Shade Dried Leafy Vegetables Consumed in Northern Côte d’Ivoire

    Directory of Open Access Journals (Sweden)

    Patricia Dehegnan Oulaï

    2016-02-01

    Full Text Available Leafy vegetables are highly perishable and often subjected to post-harvest wastes. Among the various methods of preservation, the effect of shadow drying on the nutritive value and antioxidant properties of five (5 leafy vegetables species widely consumed in Northern Côte d’Ivoire was investigated. These species were Amaranthus hybridus, Andasonia digitata, Ceiba patendra, Hibiscus sabdariffa and Vigna unguiculata. Experiment was conducted as follow: portions of washed and drained fresh leafy vegetables (500 g were spread on clean filter paper and kept in a well-ventilated room of the laboratory at 25°C for 5, 10 and 15 days. The results of proximate composition after 15 days of shadow drying were: moisture (15.19 – 20.36%, ash (9.14 -19.54%, crude fiber (11.04 – 27.40%, proteins (11.14 - 17.94%, lipids (2.41 – 5.86% and carbohydrates (16.59 – 45.14%. The concentration of minerals increased with respective values after 15 days of shadow drying: calcium (68.14-408.09 mg/100 g, magnesium (50.62-317.23 mg/100 g, iron (27.52-92.03 mg/100 g and zinc (10.17-16.73 mg/100 g. During shadow drying, vitamin C and carotenoids were subjected to losses estimated to 35.52 – 70.50% and 22.82 – 45.63%, respectively. Contrary to these losses, antioxidant activity increased and ranged from 57.45 to 75.55 % after 15 days of shadow drying. All these results suggest that the considerable nutrient contents of shade dried leafy vegetables make them good source of food supplements in order to meet the nutritional requirements of Ivorian population.

  2. Editorial-28(2-Eng

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    Yuri Morales

    2014-01-01

    Full Text Available Número actual (Vol.28 – N°1   Este número brinda al lector tres trabajos científicos en las áreas de topografía y geodesia, biología, y farmacia. Estos trabajos corresponden a manuscritos presentados en 2012 y 2013, y ofrecen aportes considerados de importancia para compartir con la comunidad nacional e internacional. El primer artículo, según los autores, describe el movimiento cinemático de una zona considerada sobre la placa Caribe, partiendo del hecho de que, precisamente, las diferencias en las series temporales, al igual que en el Sistema Geocéntrico para las Américas  (SIRGAS, se deben a los movimientos propios. El segundo trabajo, como indica el autor, tuvo como objetivo conocer, de manera sostenible, los recursos biológicos y ecológicos del Proyecto Hidroeléctrico de Toro 2. Para conocer si el sitio reúne las condiciones biológicas de atracción turística, se realizó una investigación en donde se hizo un inventario de aves, mamíferos y plantas. Por último, en el tercer artículo los autores aportan información sobre diferencias en el perfil de compuestos fenólicos y su actividad anti-radical entre diferentes comercializadores de tisanas de Hibiscus sabdariffa en Costa Rica.   Yuri Morales López Director Revista Uniciencia

  3. THRUST FORCE AND TORQUE IN DRILLING THE NATURAL FIBER REINFORCED POLYMER COMPOSITE MATERIALS AND EVALUATION OF DELAMINATION FACTOR FOR BONE GRAFT SUBSTITUTES -A WORK OF FICTION APPROACH

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    D. CHANDRAMOHAN

    2010-11-01

    Full Text Available This paper discusses about the Natural Fiber Reinforced Composite Materials contribution as bone implants. Biomaterial science is an interdisciplinary field that represents one of the most sophisticated trends in worldwide medical practice. In the last decades, researchers have developed new materials to improve the quality of human life. Owing to the frequent occurrence of bone fractures, it is important to develop a plate material for fixation on the fractured bone. These plate materials have to be lightweight, allow stiffness, and be biocompatible with humans. Drilling is the most frequently employed operation of secondary machining for fiber-reinforced materials, owing to the need for joining fractured bone by means of plate material in the field of orthopedics. An effort to utilize theadvantages offered by renewable resources for the development of biocomposite materials based on biopolymers and natural fibers has been made through fabrication of Natural fiber powdered material (Sisal (Agave sisalana, Banana (Musa sepientum, and Roselle (Hibiscus sabdariffa reinforced polymer composite plate material by using bio epoxy resin Grade 3554A and Hardner 3554B. Instead of orthopedics alloys such as Titanium, Cobalt chrome, Stainless steel, and Zirconium, this plate material can be used for internal fixationand aso external fixation on human body for fractured bone. The present work focuses on the prediction of thrust force and torque of the natural fiber reinforced polymer composite materials, and the values, compared with the Regression model and the Scheme of Delamination factor / zone using machine vision system, also discussed with the help of Scanning Electron Microscope [SEM].

  4. A quantitative documentation of the composition of two powdered herbal formulations (antimalarial and haematinic) using ethnomedicinal information from ogbomoso, Nigeria.

    Science.gov (United States)

    Ogunkunle, Adepoju Tunde Joseph; Oyelakin, Tosin Mathew; Enitan, Abosede Oluwaseyi; Oyewole, Funmilayo Elizabeth

    2014-01-01

    The safety of many African traditional herbal remedies is doubtful due to lack of standardization. This study therefore attempted to standardize two polyherbal formulations from Ogbomoso, Oyo State, Nigeria, with respect to the relative proportions (weight-for-weight) of their botanical constituents. Information supplied by 41 local herbal practitioners was statistically screened for consistency and then used to quantify the composition of antimalarial (Maloff-HB) and haematinic (Haematol-B) powdered herbal formulations with nine and ten herbs, respectively. Maloff-HB contained the stem bark of Enantia chlorantha Oliv. (30.0), Alstonia boonei De Wild (20.0), Mangifera indica L. (10.0), Okoubaka aubrevillei Phelleg & Nomand (8.0), Pterocarpus osun Craib (4.0), root bark of Calliandra haematocephala Hassk (10.0), Sarcocephalus latifolius (J. E. Smith) E. A. Bruce (8.0), Parquetina nigrescens (Afz.) Bullock (6.0), and the vines of Cassytha filiformis L. (4.0), while Haematol-B was composed of the leaf sheath of Sorghum bicolor Moench (30.0), fruit calyx of Hibiscus sabdariffa L. (20.0), stem bark of Theobroma cacao L. (10.0), Khaya senegalensis (Desr.) A. Juss (5.5), Mangifera indica (5.5), root of Aristolochia ringens Vahl. (7.0), root bark of Sarcocephalus latifolius (5.5), Uvaria chamae P. Beauv. (5.5), Zanthoxylum zanthoxyloides (Lam.) Zepern & Timler (5.5), and seed of Garcinia kola Heckel (5.5). In pursuance of their general acceptability, the two herbal formulations are recommended for their pharmaceutical, phytochemical, and microbial qualities.

  5. A Quantitative Documentation of the Composition of Two Powdered Herbal Formulations (Antimalarial and Haematinic Using Ethnomedicinal Information from Ogbomoso, Nigeria

    Directory of Open Access Journals (Sweden)

    Adepoju Tunde Joseph Ogunkunle

    2014-01-01

    Full Text Available The safety of many African traditional herbal remedies is doubtful due to lack of standardization. This study therefore attempted to standardize two polyherbal formulations from Ogbomoso, Oyo State, Nigeria, with respect to the relative proportions (weight-for-weight of their botanical constituents. Information supplied by 41 local herbal practitioners was statistically screened for consistency and then used to quantify the composition of antimalarial (Maloff-HB and haematinic (Haematol-B powdered herbal formulations with nine and ten herbs, respectively. Maloff-HB contained the stem bark of Enantia chlorantha Oliv. (30.0, Alstonia boonei De Wild (20.0, Mangifera indica L. (10.0, Okoubaka aubrevillei Phelleg & Nomand (8.0, Pterocarpus osun Craib (4.0, root bark of Calliandra haematocephala Hassk (10.0, Sarcocephalus latifolius (J. E. Smith E. A. Bruce (8.0, Parquetina nigrescens (Afz. Bullock (6.0, and the vines of Cassytha filiformis L. (4.0, while Haematol-B was composed of the leaf sheath of Sorghum bicolor Moench (30.0, fruit calyx of Hibiscus sabdariffa L. (20.0, stem bark of Theobroma cacao L. (10.0, Khaya senegalensis (Desr. A. Juss (5.5, Mangifera indica (5.5, root of Aristolochia ringens Vahl. (7.0, root bark of Sarcocephalus latifolius (5.5, Uvaria chamae P. Beauv. (5.5, Zanthoxylum zanthoxyloides (Lam. Zepern & Timler (5.5, and seed of Garcinia kola Heckel (5.5. In pursuance of their general acceptability, the two herbal formulations are recommended for their pharmaceutical, phytochemical, and microbial qualities.

  6. A Quantitative Documentation of the Composition of Two Powdered Herbal Formulations (Antimalarial and Haematinic) Using Ethnomedicinal Information from Ogbomoso, Nigeria

    Science.gov (United States)

    Ogunkunle, Adepoju Tunde Joseph; Oyelakin, Tosin Mathew; Enitan, Abosede Oluwaseyi; Oyewole, Funmilayo Elizabeth

    2014-01-01

    The safety of many African traditional herbal remedies is doubtful due to lack of standardization. This study therefore attempted to standardize two polyherbal formulations from Ogbomoso, Oyo State, Nigeria, with respect to the relative proportions (weight-for-weight) of their botanical constituents. Information supplied by 41 local herbal practitioners was statistically screened for consistency and then used to quantify the composition of antimalarial (Maloff-HB) and haematinic (Haematol-B) powdered herbal formulations with nine and ten herbs, respectively. Maloff-HB contained the stem bark of Enantia chlorantha Oliv. (30.0), Alstonia boonei De Wild (20.0), Mangifera indica L. (10.0), Okoubaka aubrevillei Phelleg & Nomand (8.0), Pterocarpus osun Craib (4.0), root bark of Calliandra haematocephala Hassk (10.0), Sarcocephalus latifolius (J. E. Smith) E. A. Bruce (8.0), Parquetina nigrescens (Afz.) Bullock (6.0), and the vines of Cassytha filiformis L. (4.0), while Haematol-B was composed of the leaf sheath of Sorghum bicolor Moench (30.0), fruit calyx of Hibiscus sabdariffa L. (20.0), stem bark of Theobroma cacao L. (10.0), Khaya senegalensis (Desr.) A. Juss (5.5), Mangifera indica (5.5), root of Aristolochia ringens Vahl. (7.0), root bark of Sarcocephalus latifolius (5.5), Uvaria chamae P. Beauv. (5.5), Zanthoxylum zanthoxyloides (Lam.) Zepern & Timler (5.5), and seed of Garcinia kola Heckel (5.5). In pursuance of their general acceptability, the two herbal formulations are recommended for their pharmaceutical, phytochemical, and microbial qualities. PMID:24701246

  7. PARAMETER KINETIKA INAKTIVASI TERMAL DAN ISOLASI Staphylococcus aureus PADA MINUMAN DARI GEL CINCAU HIJAU DAN ROSELA [Thermal Inactivation Kinetics Parameter and Isolation of Staphylococcus aureus on Drink from Green Grass Jelly and Roselle

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    Eko Hari Purnomo

    2015-07-01

    Full Text Available Information about heat resistance (D and z values of target bacteria is needed for the thermal process design on drink from Green Grass Jelly (Premna oblongifolia Merr. and Roselle (Hibiscus sabdariffa L., so it can guarantee quality and safety of the product. The objectives of this research were to isolate Staphylococcus aureus from commercial green grass jelly and to determine the D and z values of Staphylococcus aureus (from commercial product and standard clinical isolate ATCC 25923 on heating menstruum of green grass jelly and roselle. Isolation of S. aureus was done by inoculation in selective medium, D and z values of Staphylococcus aureus were assessed by heating at constant temperatures of 57, 53, 49, and 45°C during the time interval 2.5, 5, 10, and 15 minutes. The results showed that one isolate (Isolate A gave positive response of Staphylococcus aureus in isolation tests and had similar percentage with the reference culture of 41.8% using the API Staph Kit. Heat resistance of Staphylococcus aureus (represented as D value isolated from green grass jelly at constant heating temperature of D45, D49, D53 and D57 were 32.3, 17.9, 4.6, and 1.5 minutes. On the other hand, D value of isolates ATCC 25923 (standard clinical isolate at constant heating temperature of D45, D49, D53 and D57 were 18.5, 6.8, 2.9, and 1.4 minutes. The z value of isolates A and ATCC 25923 were 8.8°C and 10.8°C. Smaller z value of isolate A showed that pasteurization process can be accelerated and optimized with increasing the temperature slightly but has the same lethality effect.

  8. Drivers of Acceptance of a New Beverage in Europe

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    Ana Patricia Silva

    2016-05-01

    Full Text Available One of the challenges of studying food consumption behavior is to identify the drivers of choice for a food product. This is particularly important to design and develop new foods for which no previous information is available. Hibiscus sabdariffa L. (Bissap is an herbaceous plant and an important source of vitamins, minerals, and bioactive compounds, which confer a number of potential health benefits to derived products. The consumption of Bissap in beverage form is widespread in Africa and Asia, but not yet in Europe. Therefore, this study aimed to identify the main drivers of consumer acceptance of a traditional African beverage made from Bissap to which they had not been previously exposed. First, three focus groups (n = 22 were performed in Portugal to characterize the sensory profile of four Bissap beverages, to reveal perceptions and attitudes towards Bissap beverages, and to identify potential choice attributes. Subsequently, a full-profile conjoint analysis (n = 99 was performed, where consumers evaluated 37 Bissap beverage profiles, aiming to estimate choice attribute importance and to identify relevant market segments. Focus group findings showed that consumers would choose Bissap because they perceived as a healthy choice, and due to its novelty. The conjoint study showed an ideal profile for a Bissap beverage costing €0.99/L, <18 kcal/100 mL, packaged in Tetra-pack, light red color, and containing labeling information about antioxidants and Bissap. Four clusters of consumers were identified: price sensitive, body concerned, packaging attracted, and demanding, highlighting the most influential choice attributes: price, calories, and packaging. Findings provide useful guidance for new product development of an African product in the European lifestyle. Results might be useful from a nutraceutical point of view and to the food/beverages industry.

  9. Investigation of free amino acid, total phenolics, antioxidant activity and purine alkaloids to assess the health properties of non-Camellia tea

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    Wu Bi

    2016-03-01

    Full Text Available To find novel functional beverages from folk teas, 33 species of frequently used non-Camellia tea (plants other than Camellia were collected and compared with Camellia tea (green tea, pu-erh tea and black tea for the first time. Data are reported here on the quantities of 20 free amino acids (FAAs and three purine alkaloids (measured by UHPLC, total polyphenols (measured by Folin-Ciocalteu assay, and antioxidant activity (DPPH. The total amounts of FAAs in non-Camellia tea (0.62–18.99 mg/g are generally less than that of Camellia tea (16.55–24.99 mg/g. However, for certain FAAs, the quantities were much higher in some non-Camellia teas, such as γ-aminobutyric acid in teas from Ampelopsis grossedentata, Isodon serra and Hibiscus sabdariffa. Interestingly, theanine was detected in tea from Potentilla fruticosa (1.16±0.81 mg/g. Furthermore, the content of polyphenols in teas from A. grossedentata, Acer tataricum subsp. ginnala are significantly higher than those from Camellia tea; teas from I. serra, Pistacia chinensis and A. tataricum subsp. ginnala have remarkable antioxidant activities similar to the activities from green tea (44.23 μg/mL. Purine alkaloids (caffeine, theobromine and theophylline were not detected in non-Camellia teas. The investigation suggest some non-Camellia teas may be great functional natural products with potential for prevention of chronic diseases and aging, by providing with abundant polyphenols, antioxidants and specific FAAs.

  10. Morus alba o Hibiscus rosa-sinensis como sustituto parcial de soya en dietas integrales para conejos

    National Research Council Canada - National Science Library

    Lara, P.E; Itza, M.F; Sangines, J.R; Magana, M.A

    2012-01-01

    ... cecotrofia. Los objetivos del presente trabajo fueron: evaluar la tasa de crecimiento y el rendimiento de la canal en conejos alimentados con minibloques de harina de morera (Moras alba) o tulipan...

  11. Physical interaction between floral specialist bees Ptilothrix bombiformis (Cresson) (Hymenoptera: Apidae) enhances pollination of hibiscus (section Trionum: Malvaceae)

    Science.gov (United States)

    Specialist bees, those species with narrow dietary niches, rely on a few related species of floral hosts for food. Accordingly, specialists are thought of as being more efficient pollinators than are generalists. There is growing evidence, however, that this is not true in all cases. For example, we...

  12. Effect of Cadmium Stress on the Growth, Antioxidative Enzymes and Lipid Peroxidation in Two Kenaf (Hibiscus cannabinus L.) Plant Seedlings

    Institute of Scientific and Technical Information of China (English)

    LI Feng-tao; QI Jian-min; ZHANG Gao-yang; LIN Li-hui; FANG Ping-ping; TAO Ai-fen; XU Jian-tang

    2013-01-01

    The effects of cadmium stress on the growth, antioxidative enzymes and lipid peroxidation in two kenaf plants, Fuhong 991 and ZM412, were analysed under control (0.5-strength Hoagland’s nutrient solution) or five levels of cadmium stress (0.5-strength Hoagland’s nutrient solution containing different concentrations of Cd2+). The leaves and roots of control and cadmium-stressed plants were harvested after 3 wk. At the same Cd concentration, the Cd tolerance index of Fuhong 991 was higher than that of ZM412, indicating that Fuhong 991 may be more tolerant to Cd than ZM412. The superoxide dismutase (SOD), catalase activity (CAT) and peroxidase (POD) activities fluctuated in the leaves of the Cd-stressed plants compared to the control, whereas the glutathione reductase activity (GR) was much larger than the control for Fuhong 991, ensuring that sufficient quantities of GSH were available to respond to the cadmium stress. In comparison to the control, the dynamic tendency of the SOD, CAT and POD activities in roots of the Cd-stressed plants all increased and then declined, but the POD activity of Fuhong 991 remained nearly unchanged at all of the stress levels. The increase in the enzyme activities demonstrated that Fuhong 991 was more tolerant to cadmium than ZM 412. The lipid peroxidation was enhanced only in the leaves of Cd-stressed ZM 412. These findings indicated that antioxidative activities may play important roles in Cd-stressed Fuhong 991 and ZM 412 and that the leaf and root cell membranes of Fuhong 991 have a greater stability than those of ZM 412. For pollution monitoring purposes, the GR activity in the roots and leaves may serve as a biomarker of Cd for Fuhong 991, whereas lipid peroxidation may serve as biomarker for ZM 412.

  13. Antidepressant-like activity of anthocyanidins from Hibiscus rosa-sinensis flowers in tail suspension test and forced swim test

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    Pallavi B Shewale

    2012-01-01

    Conclusion: It can be concluded that MHR and AHR possess potential antidepressant activity (through dopaminergic, noradrenergic and serotonergic mechanisms and has therapeutic potential in the treatment of CNS disorders and provides evidence at least at preclinical levels.

  14. Effects of Plant Density, Seeding and Harvest Time on the Growth of Two Kenaf (Hibiscus Cannabinus L. Varieties

    Directory of Open Access Journals (Sweden)

    Lorenzo Barbanti

    2008-12-01

    Full Text Available Kenaf is an annual C3 multipurpose crop for the fibre and energy industry, whose growth has been widely investigated in the tropics, but not at relatively-high latitudes. This work aimed at evaluating the effects of two genotypes (Tainung 2 and Everglades 41, two plant densities (20 and 40 plants m-2, two seeding times (S1 and S2 and two harvest times (H1 and H2 on growth and its relations with climatic factors over three years (2003-2005 in Northern Italy (c. 45° N. Fitting curves for whole-plant dry biomass (DB and dry stems (DS on heat sums always gave a reliable description of the growth pattern along the season, explaining over 90% of the total variation. In general, the best-fitting models were the sigmoid and the exponential one for DB and DS, respectively. Among the four studied factors, only seeding time originated consistent growth differences among years, whereas the two varieties showed an equivalent behaviour, as well as the two densities. Furthermore, the thinner density allowed savings in the cost of seed at no prejudice for yield potential. S1 in general showed higher asymptotic yields than S2 in 2003 and 2004, while S2 consistently grew faster than S1 in all the three years. RUE showed a generally low value (e.g., 1.35 g MJ-1 for DB in S1, indicating a moisture constraint on crop growth, especially in the first year. As for the correlations, three traits, plant height, base stem diameter and fresh biomass, resulted significantly associated to DB and DS, with correlation coefficients (r ranging from 0.65 to 0.90; a higher degree of association with DB and DS was achieved by the multiple linear regressions of the same three traits (adj. R2 of about 0.85. A high dependence of DB and especially of DS on associated heat and rain (adj. R2 0.76 and 0.86, respectively was also observed in the variable environmental conditions among the three years, which attributes a non-negligible power of prediction to the two environmental parameters. These results offer the opportunity of estimating growth through the measure of non-destructive crop variables of easier assessment, and encourages their adoption, in association with that of simple climatic factors (temperatures, precipitation as tools of potential support to facilitate biomass assessments in commercial fields.

  15. Anti-hypercholesterolemic effect of kenaf (Hibiscus cannabinusL.) seed on high-fat diet Sprague dawley rats

    Institute of Scientific and Technical Information of China (English)

    Ng Shy Kai; Tee Ai Nee; Elaine Lai Chia Ling; Tan Chin Ping; Long Kamariah; Nyam Kar Lin

    2015-01-01

    Objective:To determine the antihypercholesterolemic effects of kenaf seed samples and compare with the commercial hypocholesterolemic drug on serum lipids profiles and malondialdehyde (MDA) level in the rat.Methods:Kenaf seed oil(KSO), microencapsulated kenaf seed oil(MKSO), kenaf seed extract(KSE) and defatted kenaf seed meal(DKSM) were prepared and phytochemicals screening on these samples were done prior in vivostudy.Phenolic compounds inKSE were quantified using high performance liquid chromatography.There were40(divided in eight diet groups of5) maleSprague dawley rats adapted to normal standard diet or hypercholesterolemic diet(HD) with or without the treatment of these kenaf samples for32 days.Results:All the kenaf samples exhibited to contain most of the major phytochemicals.KSE possessed gallic acid, tannic acid, catechin, benzaldehyde, benzoic acid, syringic acid, sinapic acid, ferulic acid, naringin acid, and protocatechuic acid.The significant higher(P<0.05) serum total cholesterol, low density lipoprotein cholesterol andMDA levels inHD group without treatment than the normal control group suggested the hypercholesterolemia was induced by the incorporation of cholesterol into diet.KSE exhibited higher cholesterol-lowering properties due to the significant lower(P<0.05) in serum triglycerides, total cholesterol andMDA levels.KSE showed the highest efficiency of cholesterol-lowering activity, followed byKSO,MKSO andDKSM.Conclusions:DKSM,MKSO, KSO andKSE appeared to have comparable anti-hypercholesterolemic effect with the commercial hypocholesterolemic drug.Hence, kenaf seed could be used as an alternative natural source to replace the synthetic hypocholesterolemic drugs.

  16. Reviews of roselle introduction cultivation and application%玫瑰茄引种栽培与应用研究进展

    Institute of Scientific and Technical Information of China (English)

    李秀芬; 朱建军; 张建锋; 殷丽青

    2015-01-01

    The advances of researching into roselle (Hibiscus sabdariffa)in China were reviewed in terms of introduction history,cultivation situation,active ingredients and health-care function as well as application and processing technology.The preliminary analysis found that the tropical and subtropical regions are adaptable to the growth of roselle,and the middle and lower reaches of the Yangtze River are the northern limit of roselle culti-vation due to less frost resistance;The appropriate sowing and picking periods are respectively from March to May and from September to November;Such nutrient substances as roselle pigments,roselle saponins and roselle polysaccharides can be extracted from the calyxes—main product of roselle,and they have such effects as bacteri-ostasis,antioxidation and cardiovascular protecton;Roselle has a vast market prospect in development of health beverage,purification and utilization of natural pigments,and extraction of seed oil as well as medicine,and can be planted as a pioneer crop in barren mountains and abandoned beaches.%综述了我国玫瑰茄的引种历史与栽培现状、有效成分与保健作用,以及在应用与加工等方面的研究进展。玫瑰茄适宜在热带、亚热带生长,长江中下游一带为玫瑰茄的引种栽培边界,长江以北地区栽培会受到早霜危害;适宜播种时间为3—5月,采收时间为9—11月;主要产品花萼可提取玫瑰茄色素、玫瑰茄皂甙、玫瑰茄多糖等多种营养物质;具有抑菌、抗氧化和保护心血管等作用;在保健饮料开发、天然色素提纯利用、玫瑰茄籽油榨取以及医药方面均具有广阔的市场前景,并可作为荒山荒滩先锋作物进行种植。

  17. Update of map the volcanic hazard in the Ceboruco volcano, Nayarit, Mexico

    Science.gov (United States)

    Suarez-Plascencia, C.; Camarena-Garcia, M. A.; Nunez-Cornu, F. J.

    2012-12-01

    (Hibiscus sabdariffa). Recently it has established tomato and green pepper crops in greenhouses. The regional commercial activities are concentrated in the localities of Ixtlán, Jala and Ahuacatlán. The updated hazard maps are: a) Hazard map of pyroclastic flows, b) Hazard map of lahars and debris flow, and c) Hazard map of ash-fall. The cartographic and database information obtained will be the basis for updating the Operational Plan of the Ceboruco Volcano by the State Civil & Fire Protection Unit of Nayarit, Mexico, and the urban development plans of surrounding municipalities, in order to reduce their vulnerability to the hazards of the volcanic activity.

  18. Influence of Acacia senegal agroforestry system on growth and yield of sorghum, sesame, roselle and gum in north Kordofan State, Sudan

    Institute of Scientific and Technical Information of China (English)

    Kamal Eldin Mohammed Fadl

    2013-01-01

    We examined the effects of intercropping with Acacia senegal (L.) Willd on growth and yield of sorghum (Sorghum bicolor L.),sesame (Sesamum indicum L.) and roselle (Hibiscus sabdariffa).Field experiments were conducted in El-Obeid Research farm (13°10' N; 30°12' E),North Kordofan State,Sudan,during 2002-2003 in an 11-year-old A.senegal plantation.The experimental design was randomized complete block design (RCBD) with four replications.Data were recorded for plant height (cm),fresh weight (kg·ha-1),dry weight (kg·ha-1),crop yield (kg·ha-1),and gum yield (kg·ha-1).We used Land Equivalent Ratios (LER) and simple financial analyses of gross surpluses to evaluate the productivity and profitability of the different treatments.The results indicated that A.senegal trees had a beneficial effect on crop performance and yield as well as gum yield.Significant differences (p < 0.05)were obtained for plant height,fresh weight,dry weight and crop yield.Therefore,yield of sorghum,sesame and roselle under intercropping system were 13.7%,23.8% and 20.9% higher than that obtained in the sole cropping system respectively.The highest yield increase was observed with sesame (23.8%).Gum yield (g/tree/picking) was significantly (p < 0.05) increased for sorghum,sesame and roslle under intercropping system.The highest yield of (298 g/tree/picking) was obtained when roselle was intercropped with A.senegal,while the least gum yield of (239 g·tree-1) was recorded in pure A.senegal plot.All the treatments gave land equivalent ratio (LER) of more than one-indicating the superiority of growing the field crops in intercropping over the sole cropping systems.The highest LER of 3.8 was obtained for sesame intercropped with A.senegal (Hashab),followed by 3.7,when sorghum was intercropped with A.senegal and 3.3 when roselle intercropped with A.senegal.All the treatments gave positive net revenues,the highest being for intercropped sorghum (558 SDG·ha-1) (SDG=Sudanese gienh

  19. POST-EXERCISE REHYDRATION WITH DIFFERENT BEVERAGES: PURE WATER, SPORTS DRINK, AND ROSELLE TEA

    Directory of Open Access Journals (Sweden)

    María de Lourdes Mayol Soto

    2002-06-01

    Full Text Available The purpose of the present study was to assess the suitability of roselle tea (Hibiscus Sabdariffa L. for post-exercise rehydration, compared with pure water and a sports drink. Roselle tea is a beverage widely consumed in Mexico and highly regarded because of its purported diuretic properties. Sixteen physically active male students from the University of Costa Rica, aged 17 to 23 years old, drank each one of the three treatments on different days, the same day of the week, in random order. They arrived in the laboratory euhydrated and, after a standardized breakfast and a brief rest, exercised intermittently on a stationary bicycle in a climate-controlled room (30-35°C and 85% RH until reaching 2.3% body mass loss dehydration. After a 30-min rest at room temperature, they drank a volume equivalent to 150% of their body mass loss of the drink assigned to that day, within 45 minutes. At the end of rehydration (0 min urine collection was performed and repeated every 30 min for a total of 3 hours. Each sample was measured for volume, color and density. Sample volumes were analyzed as percentage of ingested fluid. Data were analyzed with descriptive statistics and  two-way ANOVAs (repeated measures on both factors. While there were no statistically significant differences in total urine output (p = 0.084, an interaction was found between urine output, density, and color, with time (p < 0.001. Post-hoc analysis showed urine output to be higher initially with the sports drink, but it was significantly lower at time points  150 and 180 min. In addition, urine was significantly darker and denser at 0, 150, and 180 min with this drink, suggesting greater fluid conservation. From the results it is possible to conclude that roselle tea did not show a diuretic effect 3 hours after consumption, in exercise-dehydrated subjects. While it seems like rehydration is almost identical with the three drinks, urine composition and output dynamics is

  20. 越南棉花蓝病害研究进展%Researches on Cotton Blue Disease in Vietnam

    Institute of Scientific and Technical Information of China (English)

    琨泰檀宾

    2001-01-01

    棉花蓝病害(blue disease,BD)于20世纪80年代初期在越南首次发生,10多年后即成为棉花主要病害.罹病棉株表现为发育迟缓、矮小、叶片向下卷曲,产量和品质严重下降.蓝病害在越南产棉省宁城、平顺、平福的发病最重.该病为棉蚜携带病毒,其转主寄主有黄花稔(Sida acuta)、白背黄花稔(Sida rhombifolia)和玫瑰茄(Hibiscussabdariffa)等.这些转主寄主和滞留在田间的棉杆残体等是病害的传染源.单项防治措施有清洁棉田,适时播种,与绿豆或玉米轮作,叶面喷药,用"高巧"进行种子包衣等.综合防治措施为:每公斤种子用70水剂高巧5g(imidachloride有效剂量3.5g@kg-1)包衣并与绿豆轮作,及所有措施同时应用.%Blue disease (BD) occurred in Vietnam in the early 1980's and has become the most economic important disease of cotton since late 80's. Infected plant is stunt and its leaves curl downwards, yield and quality of fiber reduced. Ninh Thuan, Binh Thuan and Binh Phuoc provinces are cotton-grown areas that the most suffered from BD. It's caused by virus and transmitted by cotton aphid (Aphis gossypii) in persistent manner and by grafting. Sida acuta,Sida rhornbifolia and Hibiscus sabdariffa are alternate hosts of BD. They and cotton residues are the main reservoir of BD infection. Single practices such as field sanitation, compact sowing dates, cotton inter-crop with mung bean and maize, chemical foliar spray and seedcoating with Gaucho are effective in reduction of BD infection. The most effective in controlling BD is the complex of cotton inter-crop with mung bean and seed-coating with Gaucho 70WS 5g @ kg-1 of seed (Imidachloprid 3.5g (a. i. ) @kg-1). But only the applications of all measures together can obtain success in diminishing blue disease incidence and loss of yield at very low level.

  1. ISOLATION OF ANTHOCYANIDIN FROM WORA-WARI FLOWERS (Hibiscus rosa sinensis L. AND ITS APPLICATION AS INDICATORS OF ACID-BASE

    Directory of Open Access Journals (Sweden)

    Siti Nuryanti

    2013-11-01

    Full Text Available Wora-wari plants are easily cultivated and founded in Indonesia, also their bloomy is not seasonal. Isolation of anthocyanidin from Wora-wari was done by maceration using n-hexane, ethyl acetate and methanol-HCl 1.0% and isolation of anthocyanidin was performed by column chromatography. Identification for structure of anthocyanidin was done by UV-Vis spectrophotometer, FT-IR, 1H- and 13C-NMR along with color reagent. In the Wora-wari flowers, it has been identified the existence of anthocyanidin pelargonidin. The color change of anthocyanidin pelargonidin results in acid solution was red and base solution was green. Keywords: Wora-wari flower, anthocyanidin, acid-base indicator

  2. On Marx's theory of literature and art--An analysis of Marx's theory of literature and art in China, “Hibiscus Town”

    Institute of Scientific and Technical Information of China (English)

    申晓旭

    2016-01-01

    In the basic material and spiritual civilization highly abundant today, people in the United States to explore the origin of the hearts, but also the beautiful things combined with the general law. People start from the perspective of the theory and methods to explore and reflect the value of the arts, but also more pioneer artists to join the activities. Marxist literary theory is now a mature theoretical system of Marxist literary theory and discussion of specialized and not a complete discussion, but scattered in various chapters of his book. Marxist literary theory is the nature of literature, characteristics of the basic methods and principles, laws and social action. We must be content and form of literary works into the whole production process and the complex social relationships matter to go, in a dynamic social relations be investigated, in order to grasp the real laws and principles of literature and criticism.

  3. Influence of Salicylic Acid on In Vitro Micropropagation and Salt Tolerance in Two Hibiscus Species, H. acetosella and H. moscheutos (cv ‘Luna Red’)

    Science.gov (United States)

    Salicylic acid (SA) is a hormone-like substance that plays an important role in the regulation of plant growth and development. It has been reported to improve in vitro regeneration as well as induce abiotic stress tolerance in plants. The effects of varying SA concentrations (0, 0.5, and 1 mM) on i...

  4. Mechanism of the decreased food consumption and weight gain in rats following consumption of aqueous extract of the calyx of Hibiscus sabdariffaduring pregnancy

    Institute of Scientific and Technical Information of China (English)

    Eghosa E Iyare; Olufeyi A Adegoke; Uchenna I Nwagha

    2010-01-01

    Objective: To investigate the possible mechanisms of the decreasing fluid and food consumption followingHibiscussabdariffa(HS) consumption.Methods: On the 1st day of pregnancy, rats were randomly divided into three groups with six animals per each group. One group was given tap water, one was given with extract at 0.6 g/100 mL while the third group was given with extract at 1.8 g/100 mL as their drinking solution. All groups received normal rat chow and drinking solutionad libitum. Fluid& food intake and weight were measured daily throughout pregnancy and Na+ concentration in plasma was determined on the 18th day of pregnancy.Results: Results showed decreased fluid and food consumption, decreased weight gain and increased sodium ion concentration in plasma of rats withHS extract compared with the control group.Conclusions:Consumption of aqueous extract of the calyx ofHS during pregnancy decreases food consumption and weight gain through mechanisms that may depend on Na+ inHScontent and elevating Na+ concentration.

  5. ISOLATION OF ANTHOCYANIDIN FROM WORA-WARI FLOWERS (Hibiscus rosa sinensis L.) AND ITS APPLICATION AS INDICATORS OF ACID-BASE

    OpenAIRE

    Siti Nuryanti; Indarini Dwi Puspitasari; Ratman Ratman

    2013-01-01

    Wora-wari plants are easily cultivated and founded in Indonesia, also their bloomy is not seasonal. Isolation of anthocyanidin from Wora-wari was done by maceration using n-hexane, ethyl acetate and methanol-HCl 1.0% and isolation of anthocyanidin was performed by column chromatography. Identification for structure of anthocyanidin was done by UV-Vis spectrophotometer, FT-IR, 1H- and 13C-NMR along with color reagent. In the Wora-wari flowers, it has been identified the existence of anthocyani...

  6. Effect of electromagnetic field on okra (Hibiscus sculentus L.) developmental stages and the effect of okra extract on breast cancer cells

    OpenAIRE

    Z. Hasani Sadrabadi; A. Majd; M. Hamzeloo-Moghdam; Irian,S

    2016-01-01

    Background and objectives: Electric, magnetic and electromagnetic fields can act as stress factors with different effects on biological systems. Due to the nutritional and medicinal values, and the increasing electromagnetic radiations, the present study was performed to investigate the effects of the electromagnetic field on the developmental stages and cytotoxic properties of okra. Methods: Both dry and wet seeds were exposed to electromagnetic field with the intensities of 2 and 4 mT for 6...

  7. Effect of electromagnetic field on okra (Hibiscus sculentus L. developmental stages and the effect of okra extract on breast cancer cells

    Directory of Open Access Journals (Sweden)

    Z. Hasani Sadrabadi

    2016-04-01

    Full Text Available Background and objectives: Electric, magnetic and electromagnetic fields can act as stress factors with different effects on biological systems. Due to the nutritional and medicinal values, and the increasing electromagnetic radiations, the present study was performed to investigate the effects of the electromagnetic field on the developmental stages and cytotoxic properties of okra. Methods: Both dry and wet seeds were exposed to electromagnetic field with the intensities of 2 and 4 mT for 60 minutes. MTT assay was applied to evaluate the potential cytotoxic effects of okra extract on MCF-7 cell line. The anatomical structure of leaves in both treated and untreated (control plants were examined. Results: The results showed that a field intensity of 4 mT increased the speed of germination of wet treated seeds and the stem length of dry treated seeds. MTT assay revealed no cytotoxicity of the aqueous extracts of okra pods up to the concentration of 100 μg/mL from either the treated or the control plants towards the MCF-7 cell line. Conclusion: The results suggest that the electromagnetic fields would be able to increase the speed of germination without effects on percentage of germination.

  8. 纤维胆道镜和C臂X线机在手术治疗肾铸型结石合并肾盏多发结石中的应用价值%Application of the Choledochofiberscope and C-arm X-ray Device for the Surgical Management of Renal Cast-Calculus Complicated Calyceal Multiple Calculi

    Institute of Scientific and Technical Information of China (English)

    高强利; 张超雄; 陈昌贵

    2005-01-01

    目的:提高手术治疗肾铸型结石合并肾盏多发结石的疗效.方法:全组32例均先采用肾盂切开或肾窦内肾盂切开取石,再用纤维胆道镜在术中经肾盂切口观察和协助取出结石,最后用C臂X线机确定是否有残留结石.结果:30例取尽结石,2例肾盏内共残留3枚直径小于0.5 cm×0.5 cm的结石.结论:在术中联合应用纤维胆道镜和C臂X线机能协助取尽结石,减少肾盂粘膜的损伤和失血量,提高手术疗效并实现一机多用,经济、安全、方便.

  9. Nephrolithiasis (image)

    Science.gov (United States)

    Kidney stones result when urine becomes too concentrated and substances in the urine crystalize to form stones. Symptoms ... to move down the ureter causing intense pain. Kidney stones may form in the pelvis or calyces of ...

  10. Developmental expression of the Ca2+-binding proteins calretinin and parvalbumin at the calyx of Held of rats and mice.

    Science.gov (United States)

    Felmy, Felix; Schneggenburger, Ralf

    2004-09-01

    Ca(2+)-binding proteins of the EF-hand family are widely expressed in the CNS, and contribute to intracellular Ca(2+) buffering in neurons. In nerve terminals, Ca(2+)-binding proteins are likely to regulate transmitter release probability and synaptic short-term-plasticity. Here, we investigated the developmental expression pattern of calretinin and parvalbumin at a large excitatory synapse, the calyx of Held in the medial nucleus of the trapezoid body (MNTB) of rats and mice. We used two-colour immunofluorescence imaging with primary antibodies detecting one of the Ca(2+)-binding proteins, and a presynaptic marker protein, Rab-3A. Calretinin was found in nerve terminals of the calyx of Held, but not in postsynaptic principal cells. The presynaptic density of Calretinin staining, and the degree of colocalization with Rab-3A increased during postnatal development (P6-P31). Surprisingly, not all calyces of Held expressed calretinin. In rats, calretinin-containing calyces were irregularly interspersed with calretinin-negative calyces, whereas in mice, calretinin-positive calyces were preferentially located in the lateral portion of the MNTB. The percentage of calretinin-positive calyces increased during development, to about 75% and 20% at P30 in rats and in mice, respectively. Parvalbumin was present in the presynaptic calyces of Held and in the nerve fibres entering the MNTB, as well as in the somata of the MNTB principal neurons. An up-regulation of calretinin and parvalbumin in calyces of Held probably increases the presynaptic Ca(2+) buffering strength during postnatal development, but the unexpected heterogeneity of calretinin expression might cause differences in Ca(2+) signalling and transmitter release probability between calyces of Held.

  11. Radiological aspects of renal milk of calcium. Aspectos radiologicos de la lechada calcica renal

    Energy Technology Data Exchange (ETDEWEB)

    Perez Albelo, T.; Valles Gonzalez, H.; Torres Diaz, M.; Bonilla Arjona, A.; Baares Baudet, F. (Hospital Universitario de Canarias. La Laguna. Sta. Cruz de Tenerife (Spain))

    1993-01-01

    Renal milk of calcium is an uncommon condition consisting of the supension of innumerable microcalculi that occurs in the genitourinary tract in pyelogenic cysts, calyceal diverticuli and dilated collecting systems. Five cases, studied by means of radiography, ultrasonography and CT, are presented; the semiology of each of these explorations, all of which result in urine calcium levels that vary according to the position of the patients, is analyzed. All the cases reported here were associated with calyceal diverticuli. (Author)

  12. A Cultural Resources Reconnaissance of the Asan Flood Control Study Area, Asan, Guam,

    Science.gov (United States)

    1980-01-01

    plain. The plants dominating this area reflect the defoliation which resulted from the allied invasion in 1944 with tangan-tangan ( Leucaena ... leucocephala ), hibiscus (Hibiscus tiliaceus), and kamachile (Pithecellobium dulce) accounting for about 80 percent of the ground cover. In the upland area the

  13. Development and reproduction of ‘B' biotype Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) on four ornamentals

    Institute of Scientific and Technical Information of China (English)

    LILIN; SHUN-XIANGREN

    2005-01-01

    Effects of four commercial ornamentals on the development, survivorship and reproduction of the whitefly B biotype Bemisia tabaci (Gennadius) were studied in the laboratory (temperature 26 ±1℃; relative humidity 75%-90%; L: D 14:10). The total survivorship from egg to adult on hibiscus (Hibiscus rosa-sinensis L.), poinsettia (Euphorbia pulcherrima Will), cottonrose hibiscus (Hibiscus mutabilis L.) and variegated leafcroton (Codiaeum variegatum ‘Aucubaefolium') were 33.69%, 40.55%, 79.11%, and 29.39%,respectively. The developmental periods from egg to adult varied from 23.12 days on cottonrose hibiscus to 32.13 days on hibiscus. The average longevity of adult females ranged from 6.87 days on variegated leafcroton to 21.07 days on poinsettia. The average numbers of eggs laid per female were 9.20, 25.13, 54.45, and 26.79 on the above respective hosts. The intrinsic rates of natural increase (rm) for B biotype B. tabaci on cottonrose hibiscus was the highest. Based on life table analyses of whitefly populations, cottonrose hibiscus was the most suitable host for B biotype B. tabaci in this study.

  14. In vitro inhibitory effects of plant-based foods and their combinations on intestinal α-glucosidase and pancreatic α-amylase

    Directory of Open Access Journals (Sweden)

    Adisakwattana Sirichai

    2012-07-01

    Full Text Available Abstract Background Plant-based foods have been used in traditional health systems to treat diabetes mellitus. The successful prevention of the onset of diabetes consists in controlling postprandial hyperglycemia by the inhibition of α-glucosidase and pancreatic α-amylase activities, resulting in aggressive delay of carbohydrate digestion to absorbable monosaccharide. In this study, five plant-based foods were investigated for intestinal α-glucosidase and pancreatic α-amylase. The combined inhibitory effects of plant-based foods were also evaluated. Preliminary phytochemical analysis of plant-based foods was performed in order to determine the total phenolic and flavonoid content. Methods The dried plants of Hibiscus sabdariffa (Roselle, Chrysanthemum indicum (chrysanthemum, Morus alba (mulberry, Aegle marmelos (bael, and Clitoria ternatea (butterfly pea were extracted with distilled water and dried using spray drying process. The dried extracts were determined for the total phenolic and flavonoid content by using Folin-Ciocateu’s reagent and AlCl3 assay, respectively. The dried extract of plant-based food was further quantified with respect to intestinal α-glucosidase (maltase and sucrase inhibition and pancreatic α-amylase inhibition by glucose oxidase method and dinitrosalicylic (DNS reagent, respectively. Results The phytochemical analysis revealed that the total phenolic content of the dried extracts were in the range of 230.3-460.0 mg gallic acid equivalent/g dried extract. The dried extracts contained flavonoid in the range of 50.3-114.8 mg quercetin equivalent/g dried extract. It was noted that the IC50 values of chrysanthemum, mulberry and butterfly pea extracts were 4.24±0.12 mg/ml, 0.59±0.06 mg/ml, and 3.15±0.19 mg/ml, respectively. In addition, the IC50 values of chrysanthemum, mulberry and butterfly pea extracts against intestinal sucrase were 3.85±0.41 mg/ml, 0.94±0.11 mg/ml, and 4.41±0.15 mg/ml, respectively

  15. 60Coγ射线辐照不同麻种种子的细胞学效应%Cytological Effects on Seeds of Hibiscus cannabinus and Cannabis sativa Irradiated by 60Coγ ray

    Institute of Scientific and Technical Information of China (English)

    周立人; 吴雪琴; 魏晓飞; 李布青

    2003-01-01

    通过5种不同剂量的60Coγ射线对两个不同麻种3个品种材料的种子辐照效应研究表明,低剂量的60Coγ射线辐照能促进麻类作物根尖细胞的有丝分裂.但随着γ射线辐照剂量的增加,根尖细胞有丝分裂指数呈不同程度的下降趋势.60Coγ射线辐照能诱发根尖细胞的染色体畸变和核畸变,出现桥、断片、落后染色体、微核、双核、小核等多种畸变类型,并且辐照剂量越大,根尖细胞畸变类型越多,变异频率越高.研究结果还表明不同麻种、同一麻种不同品种对60Coγ射线的辐射敏感性不同,红麻中杂红305最强,红麻福红2号次之,大麻最弱.

  16. Effects of Formic Acid and Sucrose on Kenaf (Hibiscus cannabinus)Silage%添加蔗糖和甲酸对红麻青贮饲料品质的影响

    Institute of Scientific and Technical Information of China (English)

    白杰; 李德芳; 陈安国; 李建军; 黄思齐; 李辉; 唐慧娟

    2016-01-01

    探讨甲酸和蔗糖两种添加剂对红麻青贮的影响。以刈割后的红麻为青贮原料,添加不同计量的蔗糖(10 g/kg,15 g/kg和20 g/kg)和甲酸(3 mL/kg,6 mL/kg和9 mL/kg),并分别设空白对照,密封青贮60 d 后分析。结果显示,添加蔗糖或者甲酸能够显著提高粗蛋白含量(P<0.05)和可溶性碳水化合物含量(P<0.05)降低红麻青贮饲料的PH值,实验组pH均小于4.0,乳酸含量显著增加(P<0.05),氨态氮含量显著降低(P<0.05),实验组均没有检测到丁酸含量,青贮发酵品质良好;且在本实验研究范围内认为,添加20 g/kg的蔗糖得到的红麻青贮饲料的品质优于其他实验组,添加9 mL/kg的甲酸得到的红麻青贮饲料的品质优于其他实验组。%Experiments have been conducted to evaluate the quality of the kenaf silage with addition of sucrose and formic acid in this paper. Harvesting the kenaf after 65 -days growth,ensilage it with su-crose (10 g/kg,15 g/kg and 20 g/kg)or formic acid(3 mL/kg,6 mL/kg and 9 mL/kg)and set control groups. The silage was analyzed after 60 -days storage. The results showed that adding the sucrose or formic acid can significantly increase the crude protein content (P<0.05 )and soluble carbohydrate con-tent (P <0.05). The pH of the kenaf silage of all the treatments was less than 4.0,and lactic acid content increased significantly (P <0.05 ),ammonia nitrogen content decreased significantly (P <0.05 ),butyric acid was not detected in all of the treatments;the silage fermentation quality was in good condition. Hence,the fermentation quality and nutritive value of kenaf silage were improved with addi-tives of sucrose. The quality of kenaf silage with 20 g/kg of sucrose added is better than other groups and the quality of kenaf silage with 9 mL/kg of formic acid added is better than other groups in the range of this study.

  17. Desarrollo de un proceso a escala de laboratorio para la extracción de colorante a partir de la flor del árbol de Majagua (Hibiscus Elatus)

    OpenAIRE

    Jiménez Ramírez, Astrid Eliana

    2008-01-01

    Para desarrollar un colorante en polvo, se realizó en primera instancia un estudio de mercado preliminar, donde se analizaron los diferentes requerimientos que la industria de colorantes para alimentos está demandando en la actualidad. En este proyecto se tuvieron en consideración diferentes variables, las cuales permitieron determinar las mejores condiciones, para la operación más crítica de este proceso: la extracción sólido líquido.

  18. Rendimiento y calidad de forraje de kenaf (Hibiscus cannabinus L. en tres edades en comparación con maíz y sorgo x Sudán nervadura café

    Directory of Open Access Journals (Sweden)

    David Guadalupe Reta Sánchez

    2010-01-01

    valores de fibra detergente ácido (FDA y fibra detergente neutro (FDN fueron similares o mayores que maíz y sorgo en edades posteriores los 66 días después de la siembra (dds. La calidad de forraje en kenaf se redujo (P<0.05 con la edad al disminuir la asignación de MS a las hojas, manteniendo una calidad aceptable al inicio de floración (66 a 74 dds con contenidos de PC de 13.5 a 15.8 %, FDA de 35 a 47.5 % y FDN de 42.1 a 59.5 %.

  19. Morphological evidence for local microcircuits in rat vestibular maculae

    Science.gov (United States)

    Ross, M. D.

    1997-01-01

    Previous studies suggested that intramacular, unmyelinated segments of vestibular afferent nerve fibers and their large afferent endings (calyces) on type I hair cells branch. Many of the branches (processes) contain vesicles and are presynaptic to type II hair cells, other processes, intramacular nerve fibers, and calyces. This study used serial section transmission electron microscopy and three-dimensional reconstruction methods to document the origins and distributions of presynaptic processes of afferents in the medial part of the adult rat utricular macula. The ultrastructural research focused on presynaptic processes whose origin and termination could be observed in a single micrograph. Results showed that calyces had 1) vesiculated, spine-like processes that invaginated type I cells and 2) other, elongate processes that ended on type II cells pre- as well as postsynaptically. Intramacular, unmyelinated segments of afferent nerve fibers gave origin to branches that were presynaptic to type II cells, calyces, calyceal processes, and other nerve fibers in the macula. Synapses with type II cells occurred opposite subsynaptic cisternae (C synapses); all other synapses were asymmetric. Vesicles were pleomorphic but were differentially distributed according to process origin. Small, clear-centered vesicles, approximately 40-60 nm in diameter, predominated in processes originating from afferent nerve fibers and basal parts of calyces. Larger vesicles approximately 70-120 nm in diameter having approximately 40-80 nm electron-opaque cores were dominant in processes originating from the necks of calyces. Results are interpreted to indicate the existence of a complex system of intrinsic feedforward (postsynaptic)-feedback (presynaptic) connections in a network of direct and local microcircuits. The morphological findings support the concept that maculae dynamically preprocess linear acceleratory information before its transmission to the central nervous system.

  20. Oxidative stability and bacteriological assessment of meat from ...

    African Journals Online (AJOL)

    SERVER

    2007-12-03

    Dec 3, 2007 ... Oxidation of refrigerated meat decreased (P<0.01) with increasing levels of dietary H. sabdariffa .... tion of the number of bacteria per gram of meat product were ..... meat is fresh, it is unlikely that spoilage would have set in.

  1. Research Paper

    African Journals Online (AJOL)

    AJTCAM

    gain in both healthy and infected rats; even though the feed-intake was not ... well as hypertension (Owolabi et al., 1994), obesity and diabetes (Ajabonna and ... maintained, ad libitum, on H. sabdariffa aqueous extract that was estimated to ...

  2. 77 FR 22510 - Importation of Fresh Bananas From the Philippines Into the Continental United States

    Science.gov (United States)

    2012-04-16

    ... commercial consignments, monitoring of fruit flies to establish low- prevalence places of production... (Dymicoccus neobrevipes) Coffee root (Geococcus coffeae) Hibiscus (Maconellicoccus hirsutus) Coffee..., monitoring, and oversight of places of production; ] Trapping for the Bactrocera spp. fruit flies...

  3. Virginia Coast Reserve 2007 Remote Sensing Experiment

    Science.gov (United States)

    2012-03-09

    Chenopodium ambrosiode Helianthemum canadense Allium canadense Crataegus viridis Hibiscus moscheutos Ambrosia artemisiifoli Cynodon dactylon... laevigata Galium pilosum Mitchella Repens Cenchrus tribuloides Galium tinctorium Mollugo Verticillata 13 Genus Species Genus Species Genus

  4. 76 FR 78311 - National Register of Historic Places; Notification of Pending Nominations and Related Actions

    Science.gov (United States)

    2011-12-16

    ...), San Diego Marriot Marina, A Dock, 333 W. Harbor Dr., San Diego, 11000990 MAINE Knox County Main Street..., 74000653 Dixie Court Hotel, 301 N. Dixie Hwy., West Palm Beach, 86001723 Hibiscus Apartments, 619...

  5. Original Research

    African Journals Online (AJOL)

    home

    2014-12-23

    Dec 23, 2014 ... production of Sudan hibiscus is more profitable both by direct sawing and preparing seedlings .... the market for this plant product was not well established the price .... Ethiopian trade and industry minister should take its part.

  6. Immediate skin and mucosal symptoms from pot plants and vegetables in gardeners and greenhouse workers

    DEFF Research Database (Denmark)

    Paulsen, E; Skov, Per Stahl; Andersen, Klaus Ejner

    1998-01-01

    cacti, Stephanotis floribunda, Euphorbia pulcherrima and Gerbera reactions. Other new species implicated in immediate-type reactions included Ficus pumila, Gardenia jasminoides, Hibiscus rosa-sinensis, Campanula, Columnea, Epipremnum aureum, Pelargonium and Primula vulgaris. Because of the high...

  7. African Journal of Biotechnology - Vol 11, No 4 (2012)

    African Journals Online (AJOL)

    Effects of gibberellic acid on growth and photosynthetic pigments of Hibiscus ... Removal of heavy metals (Pb, Cu and Cr) from aqueous solutions using five plants ... Assessment of drug induced genotoxicity in gastric cancer patients · EMAIL ...

  8. Transverse comparisons between ultrasound and radionuclide parameters in children with presumed antenatally detected pelvi-ureteric junction obstruction

    Energy Technology Data Exchange (ETDEWEB)

    Duong, Hong Phuoc; Janssen, Francoise; Hall, Michelle; Ismaili, Khalid [Universite Libre de Bruxelles (ULB), Department of Pediatric Nephrology, Hopital Universitaire des Enfants Reine Fabiola, Brussels (Belgium); Piepsz, Amy [Hopital Universitaire Saint-Pierre, Department of Radioisotopes, Ghent (Belgium); Khelif, Karim; Collier, Frank [Universite Libre de Bruxelles (ULB), Department of Pediatric Urology, Hopital Universitaire des Enfants Reine Fabiola, Brussel (Belgium); Man, Kathia de [University Hospital Ghent, Department of Nuclear Medicine, Ghent (Belgium); Damry, Nash [Universite Libre de Bruxelles (ULB), Department of Pediatric Radiology, Hopital Universitaire des Enfants Reine Fabiola, Brussel (Belgium)

    2015-05-01

    The main criteria used for deciding on surgery in children with presumed antenatally detected pelviureteric junction obstruction (PPUJO) are the level of hydronephrosis (ultrasonography), the level of differential renal function (DRF) and the quality of renal drainage after a furosemide challenge (renography), the importance of each factor being far from generally agreed. Can we predict, on the basis of ultrasound parameters, the patient in whom radionuclide renography can be avoided? We retrospectively analysed the medical charts of 81 consecutive children with presumed unilateral PPUJO detected antenatally. Ultrasound and renographic studies performed at the same time were compared. Anteroposterior pelvic diameter (APD) and calyceal size were both divided into three levels of dilatation. Parenchymal thickness was considered either normal or significantly decreased. Acquisition of renograms under furosemide stimulation provided quantification of DRF, quality of renal drainage and cortical transit. The percentages of patients with low DRF and poor drainage were significantly higher among those with major hydronephrosis, severe calyceal dilatation or parenchymal thinning. Moreover, impaired cortical transit, which is a major risk factor for functional decline, was seen more frequently among those with very severe calyceal dilatation. However, none of the structural parameters obtained by ultrasound examination was able to predict whether the level of renal function or the quality of drainage was normal or abnormal. Alternatively, an APD <30 mm, a calyceal dilatation of <10 mm and a normal parenchymal thickness were associated with a low probability of decreased renal function or poor renal drainage. In the management strategy of patients with prenatally detected PPUJO, nuclear medicine examinations may be postponed in those with an APD <30 mm, a calyceal dilatation of <10 mm and a normal parenchymal thickness. On the contrary, precise estimation of DRF and renal

  9. Sound-sensitive neurons innervate the ventro-lateral protocerebrum of the heliothine moth brain

    DEFF Research Database (Denmark)

    Pfuhl, Gerit; Zhao, Xin Cheng; Ian, Elena

    2014-01-01

    soma positioned near the calyces of mushroom bodies and with numerous neuronal processes in the ventro-lateral protocerebrum. Mass-staining of ventral-cord neurons supported the assumption that the ventro-lateral region of the moth brain was the main target for the auditory projections ascending from...

  10. A Rare Complication of Percutaneous Nephrolithotomy: Biliary Peritonitis

    Directory of Open Access Journals (Sweden)

    Sadi Turkan

    2014-04-01

    Full Text Available Percutaneous nephrolithotomy is a common method in stone therapy in the last 30 years. High success rates as well as high complication rates are also available. We present offer patient who developing gallbladder injury and peritonitis in percutaneous nephrolithotomy for the right renal pelvis, mid, and lower calyces stone.

  11. A Rare Complication of Percutaneous Nephrolithotomy: Biliary Peritonitis

    OpenAIRE

    Sadi Turkan

    2014-01-01

    Percutaneous nephrolithotomy is a common method in stone therapy in the last 30 years. High success rates as well as high complication rates are also available. We present offer patient who developing gallbladder injury and peritonitis in percutaneous nephrolithotomy for the right renal pelvis, mid, and lower calyces stone.

  12. Post-tetanic potentiation in the rat calyx of Held synapse.

    NARCIS (Netherlands)

    R.L.P. Habets (Ron); J.G.G. Borst (Gerard)

    2005-01-01

    textabstractWe studied synaptic plasticity in the calyx of Held synapse, an axosomatic synapse in the auditory brainstem, by making whole-cell patch clamp recordings of the principal cells innervated by the calyces in a slice preparation of 7- to 10-day-old rats. A 5 min 20 Hz stimulus train

  13. Dynamic development of the calyx of Held synapse

    NARCIS (Netherlands)

    A. Rodríguez-Contreras (Adrián); J.S.S. van Hoeve (John); R.L.P. Habets (Ron); H. Locher (Heiko); J.G.G. Borst (Gerard)

    2008-01-01

    textabstractThe calyx of Held is probably the largest synaptic terminal in the brain, forming a unique one-to-one connection in the auditory ventral brainstem. During early development, calyces have many collaterals, whose function is unknown. Using electrophysiological recordings and fast-calcium

  14. Detailed evaluation of the upper urinary tract in patients with prune belly syndrome using magnetic resonance urography.

    Science.gov (United States)

    Garcia-Roig, M L; Grattan-Smith, J D; Arlen, A M; Smith, E A; Kirsch, A J

    2016-04-01

    Magnetic resonance urography (MRU) has proven to be useful in the setting of complex urologic anatomy. Prune belly syndrome (PBS) patients are known to have malformed and highly variable urinary tract anatomy due to significant dilation and renal dysplasia. To further characterize the renal and ureteral anatomy and renal function in patients with PBS via MRU. Children with PBS undergoing MRU (2006-2011) were identified. Studies were performed to evaluate severe hydronephrosis in all patients. Demographics, previous imaging, and MRU findings were collected. A single radiologist reviewed all studies. MRU was performed on 13 boys, with a median age of 29.3 months (IQR 6-97). Two patients underwent >1 study for ureteropelvic junction obstruction (UPJ obstruction) and calyceal diverticulum with a solitary kidney, respectively. Hydroureteronephrosis (HUN) was identified in 12 boys (92%), while one (8%) did not have ureteral dilation. All patients demonstrated morphologic abnormalities beyond HUN as follows: five (38%) renal dysplasia; five (38%) scarring; four (31%) calyceal diverticula; and three (23%) thickened bladder. The median renal transit time (RTT) was 6 min (IQR 3.5-10.5), and >8 min (range 8.5-35) in six patients; one patient was ultimately diagnosed with obstruction. The mean serum creatinine was 0.5 ± 0.3 mg/dl. This summary figure is a coronal excretory phase T1 MRU image demonstrating absence of well-defined calyces and a 5-cm calyceal diverticulum (white arrow). This study reports significant anatomic and functional findings on MRU that were not readily apparent when using standard imaging for children with PBS. The high-resolution images and functional data obtained with MRU allowed for visualization of calyceal diverticula and abnormal renal pelvic anatomy not previously described in PBS. In addition, renal dysplasia could be identified with MRU, which is badly characterized in the PBS population outside of renal biopsy studies. Potential limitations

  15. COMPARISON OF ROLE OF IVP AND USG IN EVALUATING URETERIC CALCULI DISEASE

    Directory of Open Access Journals (Sweden)

    Rajesh Rathore

    2016-07-01

    Full Text Available The incidence of urolithiasis varies according to geographic area, age, and sex distribution. 1 Various studies have shown that in economically developed countries the prevalence rate ranged between 4% and 20%. Urolithiasis may affect up to 10% of the population over the course of a lifetime. In India, upper and lower urinary tract stones occur frequently, but the incidence shows wide regional variation. Smaller stones (Up to 5 mm may cause severe symptoms such as flank pain and nausea, but pass without intervention. Though, they may need supportive care, but larger stones need intervention. Surgery has been used to remove stones (Lithotomy or to break them all into small pieces (Lithotripsy since antiquity. Twenty five years ago, open surgery was the sole form of therapy for urolithiasis, but now endourology is the surgical treatment of choice. 2 URETERIC JET When the bolus of urine being transmitted through the ureter reaches the terminal portion. It is ejected forcefully into the bladder through the Vesicoureteric Junction (VUJ. This creates a jet of urine that can be seen within the urinary bladder during cystoscopy and grey-scale Ultrasonography (US. Urine secreted from the renal glomeruli is excreted into the upper urinary tract including the collecting system, calyces-pelvis system, and ureter. Although, the precise mechanism of urine drainage through the whole system remains somewhat contentious. The trigger of ureteric jets is undoubtedly the pumping effect of calyceal peristalsis. 3 The latter comprises an autonomic and rhythmic minor calyceal peristalsis and a pressure-dependent major calyceal peristalsis, and the pressure of the major calyces comes from the resistance of the intrinsic tone of the upper ureter to the urine draining in the renal pelvic volume and tone and the rate of urine inflow. 4 Continuous discrete boluses of urine drainage into the ureter from pelvis, then fill the ureter, and finally, a ureteric jet is produced

  16. Population growth, survival and devolopment of Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) fed on different Hibiscus rosa-sinensis leaves%不同类型扶桑叶片上棉花粉蚧存活、发育和增长能力

    Institute of Scientific and Technical Information of China (English)

    王超; 何沐阳; 陆永跃

    2016-01-01

    为了明确以不同成熟度扶桑叶片为食的棉花粉蚧种群增长能力,观察、比较了扶桑嫩叶、成叶、老叶上该蚧不同虫期的存活率、发育历期、成虫性比和雌成虫繁殖能力.结果表明,一、二龄若虫存活率随着叶片成熟度上升而下降,由96.38%、93.56%分别降低至81.14%、89.13%,而不同叶片上三龄若虫存活率无显著差异.嫩叶上一龄若虫发育历期(4.92 d)显著短于成叶(5.67 d)、老叶(5.93 d),而二龄、三龄若虫发育历期无明显变化.取食成叶的棉花粉蚧雌虫产仔量最高,为361.78头/雌,显著高于取食嫩叶(305.1头/雌)和老叶(319.79头/雌).子代中雌虫比例以成叶最高,为0.66,嫩叶和老叶较低,分别为0.61、0.54.

  17. Toxic effects of four insecticides on mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) fed on Hibiscus rosa-sinensis (Malvales: Malvaceae)%4种杀虫剂对取食朱槿的扶桑绵粉蚧的毒杀作用

    Institute of Scientific and Technical Information of China (English)

    王伟兰; 陈红松; 黄立飞; 姜建军; 杨朗

    2014-01-01

    扶桑绵粉蚧已入侵广西,并在朱槿上成功定殖,且对朱槿造成一定的为害,研究杀虫剂对其毒杀作用,可为其大暴发时的应急防治提供参考.采用浸叶法测定了丙溴磷、毒死蜱、吡虫啉、印楝油对取食朱槿的扶桑绵粉蚧的毒杀效果,用Probit Analysis建立毒力回归方程,比较了扶桑绵粉蚧对不同杀虫剂的敏感性.结果表明,印楝油对扶桑绵粉蚧的直接毒杀作用较弱,药后3d最高浓度(10 mL/L)对一龄若虫的致死率仅为66.43%,对其他3个虫龄几乎无直接毒杀作用;另外3种化学杀虫剂均具较强的毒杀作用,药后3d,大多数试验浓度的校正死亡率在50.00%以上,最高浓度的死亡率均超过90.00%;不同虫龄的敏感性顺序为一龄>二龄>三龄>雌成虫,药剂的敏感性顺序为丙溴磷>吡虫啉>毒死蜱.说明化学杀虫剂对扶桑绵粉蚧的直接毒杀效果显著,但不同虫龄对不同药剂的敏感性差异较大,田间用药宜根据虫龄结构,选择合适的药剂、剂量和时机.

  18. Interspecific Hybridization within Ornamental Plants

    DEFF Research Database (Denmark)

    Kuligowska, Katarzyna

    The economic importance of the ornamental plant industry requires constant development of novel and high quality varieties. Traits attractive for production of new ornamental plants may not be available within the commercial cultivars, but broad genetic variation is present within the plant genera...... commercially important genera of ornamental plants: Kalanchoë and Hibiscus. The nature of hybridization barriers hampering hybrid production was investigated during pre- and post-fertilization stages. For each genus the interspecific crosses of Kalanchoë species and Hibiscus species, abnormal germination...

  19. Ethnobotanical relevance in tribal life: A study on Warli tribe of Thane district, Maharashtra, India

    Digital Repository Service at National Institute of Oceanography (India)

    Pereira, N.

    . orientalis (L.) Merr.) are used to heal pus fined boils. While leaf of Jaswand (Hibiscus rosa-sinensis L,) is used for effective healing of wounds. Leaf of Hadmodi (Kalanchoe pinnuta (Lam.) Pers.) is known for its mazing property of binding the skin.... (Lam.) Raynal) leaves is given 2 Skin infections children with this problem. For relief from biles and acidity, r I Crushed leaves of Jaswand (Hibiscus fruit of Karela (Mornordica cha rosa-sinsnsis L.) are used to cure Linn.) is crushed...

  20. Screening of medicinal plants from Suriname for 5-HT(1A) ligands: Bioactive isoquinoline alkaloids from the fruit of Annona muricata.

    Science.gov (United States)

    Hasrat, J A; Pieters, L; De Backer, J P; Vauquelin, G; Vlietinck, A J

    1997-06-01

    Plants from Suriname (South-America) and several Annona species, including A. muricata, A. ckerimolia, A. montana and A. glabra were screened for 5-HT(1A) receptor binding activity by ligand-binding-studies (LBS). Crude extracts of all Annona species and from Hibiscus bifurcatus, Irlbarchia purpurascens and Scoparia dulcis showed high activity. The isoquinoline alkaloids asimilobine (1), nornuciferine (2), and annonaine (3) were isolated as the active principles from the fruit of Annona muricata. These results may partially explain the use of Hibiscus bifurcatus and Annona muricata in traditional medicine in Suriname.

  1. Interspecific Hybridization within Ornamental Plants

    DEFF Research Database (Denmark)

    Kuligowska, Katarzyna

    The economic importance of the ornamental plant industry requires constant development of novel and high quality varieties. Traits attractive for production of new ornamental plants may not be available within the commercial cultivars, but broad genetic variation is present within the plant genera...... commercially important genera of ornamental plants: Kalanchoë and Hibiscus. The nature of hybridization barriers hampering hybrid production was investigated during pre- and post-fertilization stages. For each genus the interspecific crosses of Kalanchoë species and Hibiscus species, abnormal germination...

  2. Evaluation of renal function using Gd-DTPA dynamic MR imaging

    Energy Technology Data Exchange (ETDEWEB)

    Furukawa, Akira; Murata, Kiyoshi; Morita, Rikushi [Shiga Univ., Otsu (Japan). Medical Science

    1996-04-01

    To establish a new method for evaluating renal function using MRI, we performed dynamic Gd-DTPA enhanced MR imaging at 1.5 T in 68 subjects (71 examinations), including 23 normal volunteers, 19 patients with chronic renal failure, 22 with hydronephrosis, and 7 with renal vascular disorders. Thirty GRASS images [35/9/20deg/1 (TR/TE/Flip Angle/NEX)] were obtained after a bolus injection of Gd-DTPA (0.2 mmol/Kg) in each case, and were evaluated in the following respects. Whether or not the initial signal increase and/or the following signal drop in cortex was observed. Whether or not the signal drop in medulla and/or calyces was observed. Time between initial signal increase in cortex and signal drop in medulla (Ta). Time between signal drop in medulla and calyces (Tb). Time between signal increase in cortex and signal drop in calyces (Tc). Maximum ratio of signal intensity between cortex and medulla(Max. C/M). In normal subjects, the initial signal increase and the following signal drop in cortex, and the signal drop in medulla and calyces were observed. In patients with abnormal renal function, the characteristic signal changes discribed above were not seen clearly or disappeared in many cases. In normal subjects, Ta, Tb, Tc, and Max. C/M were 41.9{+-}6.9 sec, 53.2{+-}10.6 sec, 95.0{+-}9.5 sec, and 1.10{+-}0.05, respectively. In the patients, time parameters were significantly larger than those of normal subjects and C/M ratios were lower. This study suggested that dynamic Gd-DTPA renal MR imaging can serve as a new method for the evaluation of renal function. (author).

  3. The development rhythm of the flower-bud in some Papilionaceae species. III. Macrosporogenesis, microsporogenesis and early gametogenesis in several species of the Vicieae tribe

    Directory of Open Access Journals (Sweden)

    Wanda Wojciechowska

    2014-01-01

    Full Text Available Each of the examined species of the tribe Vicieae (Vicia faba, V. sativa, V, villosa, Lathyrus silvester, L. pratensis and Pisum sativum has its peculiar characteristic development rhythm of the bud. A similarity has been demonstrated between the development rhythms of flower buds of Vicia faba and Pisum sativum. It was found that mature flowers of autogamous species had long calyces, whereas those of the allogamous species were short as compared with the petals of the corolla.

  4. Acute management of stones

    DEFF Research Database (Denmark)

    Jung, Helene; Osther, Palle J S

    2015-01-01

    INTRODUCTION: Stone management is often conservative due to a high spontaneous stone passage rate or non-symptomatic calyceal stones that do not necessarily require active treatment. However, stone disease may cause symptoms and complications requiring urgent intervention. MATERIAL AND METHODS......: In this review, we update latest research and current recommendations regarding acute management of stones, with particular focus on imaging, pain management, active stone interventions, medical expulsive therapy, and urolithiasis in pregnancy and childhood. RESULTS: Acute stone management should be planned...

  5. [The conservative treatment of phosphate calculi by citrate buffer (author's transl)].

    Science.gov (United States)

    Zechner, O; Höltl, G

    1975-05-02

    3 patients with U-tube pyelonephrostomy were treated conservatively for multiple struvite (triple phosphate) stones in the kidney by means of constant irrigation with citrate buffer (pH 4.8). Complete dissolution of the stones was achieved in 2 patients. Conservative therapy was unsuccessful in the third case in view of narrowing of the renal calyces. Combined therapy by means of direct irrigation and oral chemotherapy is recommended.

  6. Parvalbumin is a mobile presynaptic Ca2+ buffer in the calyx of Held that accelerates the decay of Ca2+ and short-term facilitation.

    Science.gov (United States)

    Müller, Martin; Felmy, Felix; Schwaller, Beat; Schneggenburger, Ralf

    2007-02-28

    Presynaptic Ca2+ signaling plays a crucial role in short-term plasticity of synaptic transmission. Here, we studied the role of mobile endogenous presynaptic Ca2+ buffer(s) in modulating paired-pulse facilitation at a large excitatory nerve terminal in the auditory brainstem, the calyx of Held. To do so, we assessed the effect of presynaptic whole-cell recording, which should lead to the diffusional loss of endogenous mobile Ca2+ buffers, on paired-pulse facilitation and on intracellular Ca2+ concentration ([Ca2+]i) transients evoked by action potentials. In unperturbed calyces briefly preloaded with the Ca2+ indicator fura-6F, the [Ca2+]i transient decayed surprisingly fast (tau(fast), approximately 30 ms). Presynaptic whole-cell recordings made without additional Ca2+ buffers slowed the decay kinetics of [Ca2+]i and paired-pulse facilitation (twofold to threefold), but the amplitude of the [Ca2+]i transient was changed only marginally. The fast [Ca2+]i decay was restored by adding the slow Ca2+ buffer EGTA (50-100 microM) or parvalbumin (100 microM), a Ca2+-binding protein with slow Ca2+-binding kinetics, to the presynaptic pipette solution. In contrast, the fast Ca2+ buffer fura-2 strongly reduced the amplitude of the [Ca2+]i transient and slowed its decay, suggesting that the mobile endogenous buffer in calyces of Held has slow, rather than fast, binding kinetics. In parvalbumin knock-out mice, the decay of [Ca2+]i and facilitation was slowed approximately twofold compared with wild-type mice, similar to what is observed during whole-cell recordings in rat calyces of Held. Thus, in young calyces of Held, a mobile Ca2+ buffer with slow binding kinetics, primarily represented by parvalbumin, accelerates the decay of spatially averaged [Ca2+]i and paired-pulse facilitation.

  7. Molecular microdomains in a sensory terminal, the vestibular calyx ending

    Science.gov (United States)

    Lysakowski, Anna; Gaboyard-Niay, Sophie; Calin-Jageman, Irina; Chatlani, Shilpa; Price, Steven D.; Eatock, Ruth Anne

    2011-01-01

    Many primary vestibular afferents form large cup-shaped postsynaptic terminals (calyces) that envelope the basolateral surfaces of type I hair cells. The calyceal terminals both respond to glutamate released from ribbon synapses in the type I cells and initiate spikes that propagate to the afferent’s central terminals in the brainstem. The combination of synaptic and spike initiation functions in these unique sensory endings distinguishes them from the axonal nodes of central neurons and peripheral nerves, such as the sciatic nerve, which have provided most of our information about nodal specializations. We show that rat vestibular calyces express an unusual mix of voltage-gated Na and K channels and scaffolding, cell adhesion, and extracellular matrix proteins, which may hold the ion channels in place. Protein expression patterns form several microdomains within the calyx membrane: a synaptic domain facing the hair cell, the heminode abutting the first myelinated internode, and one or two intermediate domains. Differences in the expression and localization of proteins between afferent types and zones may contribute to known variations in afferent physiology. PMID:21734302

  8. Efficacy of Isaria fumosorosea Wize (Hypocreales: Cordycipitaceae) on the leaf phylloplane over time for controlling Madeira mealybug nymphs preshipping

    Science.gov (United States)

    The efficacy of Isaria fumosorosea (= PFR 97®) on the leaf phylloplane over time for controlling Madeira mealybug nymphs before shipping plant products was assessed under laboratory conditions. Hibiscus leaves were dipped into beakers filled with 0, 1, 2, 4, 8, and 10 g of PFR 97® / L of water and t...

  9. Impact of kenaf extracts on germination of green bean, tomato, cucumber, and Italian ryegrass

    Science.gov (United States)

    The chemical interaction between plants, referred to as allelopathy, may result in the inhibition of plant growth and development. The objective of this research was to determine the impact of kenaf (Hibiscus cannabinus L.) plant extracts on the seed germination of five plant species. Four concentra...

  10. Confined Disposal Facility at Pointe Mouillee for Detroit and Rouge Rivers.

    Science.gov (United States)

    1974-03-01

    21. Common Merganser (Mergus merganser) 22. Ruddy Duck (Oxyura jamaicensis) 23. Common Scoter (Oidemia nigra ) 24. White-winged Scoter (Melanitta...Herring Gull (Larus argentatus) 5. Common Tern (Sterna hirundo hirundo) 6. Caspian Tern (Hydroprogne caspia) 7. Black Tern (Chlidonias nigra ...Hibiscus) Trees Silver Maple (Acer saccharinum) none Box Elder (Acer negundo) Apple (Malus) Peach (Prunus persica) Mulberry ( Morus ) Bronze Beech

  11. Supraventricular Tachycardia Atackt Due to Losewieght Drug

    Directory of Open Access Journals (Sweden)

    Murat Yalcin

    2016-01-01

    Full Text Available Obesity is an important health problem. Treatment of obesity includes diet, exercise and drugs. Some of these drugs are out of prescription. Advers effects of these drugs have not been known. In this report; we present a case with supraventricular tachycardia attack due to loseweight drug containing mangostana (mango, hibiscus, citrus mate, L-karnitin, guarana.

  12. Kenaf Fibre Crop for Bioeconomic Industrial Development

    NARCIS (Netherlands)

    Lips, S.J.J.; Dam, van J.E.G.

    2013-01-01

    Kenaf (Hibiscus cannabinus L.) is a high yielding fibre crop that can be utilised as raw material in many industrial applications ranging from traditional fabrics,
    yarns and ropes to new applications in building materials, composites and lightweight car parts. Kenaf competes in some applications

  13. Exploring maize-legume intercropping systems in Southwest Mexico

    NARCIS (Netherlands)

    Flores-Sanchez, D.; Pastor, A.V.; Lantinga, E.A.; Rossing, W.A.H.; Kropff, M.J.

    2013-01-01

    Maize yields in continuous maize production systems of smallholders in the Costa Chica, a region in Southwest Mexico, are low despite consistent inputs of fertilizers and herbicides. This study was aimed at investigating the prospects of intercropping maize (Zea mays L.) and maize-roselle (Hibiscus

  14. Cameroun

    African Journals Online (AJOL)

    SARAH

    activité antibactérienne de quatre .... Hypertension artérielle : infusion des feuilles et du bulbe ..... Meurtrissure, choc physique, angine, plaies : feuilles .... adulte. Hibiscus sabdarifa L. (Malvaceae). Oseille de guinée. (foléré). 1.

  15. Localisation of chlorophyll within the chloroplast

    NARCIS (Netherlands)

    Thomas, J.B.; Post, L.C.; Vertregt, N.

    1954-01-01

    Silver nitrate reduction was shown to occur in illuminated suspensions of Hibiscus grana. The action spectrum of this reduction, the reaction, proved to coincide satisfactorily with the chlorophyll absorption spectrum. Electron micrographs reveal that this reaction occurs in single lamellae. From

  16. Mechanical properties and surface chemistry of kenaf fiber for composite reinforcement – an insight into the retting process

    Science.gov (United States)

    Harnessing natural fibers to produce polymer composites requires processing of fibers from harvest to the dried state, which can then be dispersed in the polymer resin. Bast fibers are found in the bark layer of fibrous plants such as kenaf (Hibiscus cannabinus), jute (Corchorus olitorius), and flax...

  17. Some engineering properties of white kidney beans (Phaseolus ...

    African Journals Online (AJOL)

    ajl yemi

    2011-12-19

    Dec 19, 2011 ... average, the bean contains 21.7 g protein, 0.75 g oil, 55.2 g total carbohydrates ..... some physico-chemical properties of Turkey okra (Hibiscus esculenta. L.) seeds. ... major commercial Turkish hazelnut varieties. J. Food Eng.

  18. Efficiencies in alginate encapsulation of vegetative explants

    Science.gov (United States)

    The goal of this study was to improve a non-mechanized bulk encapsulation technique to standardize encapsulation procedures and reduce the labor time compared to encapsulating individual nodes. Four mm-long nodal segments from Stage II cultures of Hibiscus moscheutos L. ‘Lord Baltimore’ were encapsu...

  19. Sharing information and collections on phytoplasmas: from QBOL to QBANK

    DEFF Research Database (Denmark)

    Bertaccini, Assunta; Paltrinieri, Samanta; Makarova, Olga

    2011-01-01

    A total of 154 phytoplasma strains from 15 ribosomal groups were employed for barcode sequences production. Besides strains in periwinkle, 36 strains in natural infected plants such as napier grass, grapevine, plum, jujube, apple, pear, spartium, pine tree, hibiscus and erigeron were employed. Ba...

  20. Kenaf and bioremediation in Azerbaijan: Planting locations, plant populations, and varieties

    Science.gov (United States)

    Scientists, businesses, and the Azerbaijan government are bringing together two ancient natural resources, petroleum and kenaf (Hibiscus cannabinus L.), to create a balance between economic growth and environmental reclamation. Oil and kenaf have been used for over 3000 years. Although oil continu...

  1. Kenaf and bioremediation in Azerbaijan

    Science.gov (United States)

    Scientists, businesses, and the Azerbaijan government are bringing together two ancient natural resources, petroleum and kenaf (Hibiscus cannabinus L.), to create a balance between economic growth and environmental reclamation. Oil and kenaf have been used for over 2000 years. Although oil continu...

  2. Kenaf Fibre Crop for Bioeconomic Industrial Development

    NARCIS (Netherlands)

    Lips, S.J.J.; Dam, van J.E.G.

    2013-01-01

    Kenaf (Hibiscus cannabinus L.) is a high yielding fibre crop that can be utilised as raw material in many industrial applications ranging from traditional fabrics,
    yarns and ropes to new applications in building materials, composites and lightweight car parts. Kenaf competes in some applications

  3. Phenology of Maconellicoccus hirsutus (Green) Hemiptera: Pseudococcidae) in Florida based on attraction of adult males to pheromone traps

    Science.gov (United States)

    Research was conducted in Florida to assess the phenology of pink hibiscus mealybug, Maconellicoccus hirsutus (Green), based on numbers of adult males captured at traps baited with a synthetic pheromone. Trapping was conducted at three locations in east central Florida in ornamental plantings of hib...

  4. Dye strip dosimeter

    Science.gov (United States)

    Saisomboon, S.; Siri-Upathum, C.

    1987-10-01

    This paper describes a new method for measuring radiation dose by using natural pigments. The pigments were extracted from Hibiscus rosa sinensis L. and Canna indica L. and were irradiated with gamma ray. Doses of 30 rad and above are indicated by color changes.

  5. Antipityrosporum Ovale Activity Of A Herbal Drug Combination Of Wrightia Tinctoria And Hisbiscus Rosasinensis

    Directory of Open Access Journals (Sweden)

    Kirshnamoorthy J R

    2000-01-01

    Full Text Available Antipityosporum activity of a herbal drug combination of Wrighria tinctoria and Hibiscus rosasinensis was tested in vitro against the isolates of Pityrosporum ovale recovered from dandruff. The drug combination exhibited fungicidal activity at a concentration ranging between 500 to1000 pg/ml.

  6. 78 FR 8957 - Importation of Fresh Bananas From the Philippines into the Continental United States

    Science.gov (United States)

    2013-02-07

    ... coffeae (Green), the coffee root mealybug; Maconellicoccus hirsutus (Green), the hibiscus mealybug... establish low- prevalence places of production, harvesting only of hard green bananas, and inspection for...; Covering bananas with pesticide bags during the growing season; Harvesting only of hard green...

  7. Regulation of 4CL, encoding 4-coumarate: coenzyme A ligase, expression in kenaf under diverse stress conditions

    Science.gov (United States)

    We cloned the full length 4CL ortholog encoding 4-coumarate: coenzymeA ligase from kenaf (Hibiscus cannabiuns) using degenerate primers and RACE (rapid amplification of cDNA ends) systems. The 4CL is a key regulatory enzyme of the phenylpropanoid pathway that regulates the activation of cinnamic ac...

  8. Expression analysis of kenaf cinnamate 4-hydroxylase (C4H) ortholog during developmental and stress responses

    Science.gov (United States)

    This study was conducted to clone and analyze the expression pattern of a C4H gene encoding cinnamate 4-hydroxylase from kenaf (Hibiscus cannabinus L.). A full-length C4H ortholog was cloned using degenerate primers and the RACE (rapid amplification of cDNA ends) method. The full-length C4H ortholog...

  9. Sharing information and collections on phytoplasmas: from QBOL to QBANK

    DEFF Research Database (Denmark)

    Bertaccini, Assunta; Paltrinieri, Samanta; Makarova, Olga

    2011-01-01

    A total of 154 phytoplasma strains from 15 ribosomal groups were employed for barcode sequences production. Besides strains in periwinkle, 36 strains in natural infected plants such as napier grass, grapevine, plum, jujube, apple, pear, spartium, pine tree, hibiscus and erigeron were employed. Ba...

  10. 77 FR 66938 - Special Local Regulations; 2012 Holiday Boat Parades, Captain of the Port Miami Zone; FL

    Science.gov (United States)

    2012-11-08

    ... Lauderdale, Pompano Beach, Palm Beach, Boynton Beach, Delray Beach, and Miami, Florida. These special local... will be enforced from 5:00 p.m. until 10:00 p.m. on December 9, 2012. 3. Palm Beach, Florida. On... around Palm Island and Hibiscus Island, head east between Di Lido Island, south through Meloy...

  11. 77 FR 70681 - Special Local Regulations; 2012 Holiday Boat Parades, Captain of the Port Miami Zone; FL

    Science.gov (United States)

    2012-11-27

    ..., Florida. On December 1, 2012, Marine Industries Association of Palm Beach County is sponsoring the Palm... regulation will be enforced from 5:00 p.m. until 10:00 p.m. on December 9, 2012. (3) Palm Beach, Florida. All... around Palm Island and Hibiscus Island, head east between Di Lido Island, south through Meloy...

  12. Rigid polyurethane foam – kenaf core composites for structural applications

    Science.gov (United States)

    Kenaf (Hibiscus cannabinus L.) is a fast growing summer annual crop with numerous commercial applications (fibers, biofuels, bioremediation, paper pulp, building materials, cover crops, and livestock forages). The stalks of the kenaf plants contain two distinct fiber types, bast and core fibers. The...

  13. Kenaf’s allelopathic impact on seedling growth

    Science.gov (United States)

    Allelopathy is the chemical interaction between plants, which may result in the inhibition of plant growth and development. Research was conducted to determine the impact of kenaf (Hibiscus cannabinus L.) plant extracts on the post-germination growth of five plant species. Four concentrations (0, 16...

  14. Response of Gas-exchange Parameters to Light Intensity and Temperature of Sun and Shade Leaves in Hibiscusmutabilis%阴、阳生木芙蓉气体交换参数对光温的响应

    Institute of Scientific and Technical Information of China (English)

    蒋怡珊; 张边江; 唐宁; 陈全战; 杨平

    2013-01-01

    以木芙蓉(Hibiscusmutabilis)为实验材料,研究阴、阳生木芙蓉气体交换参数对光、温的响应。测定了不同光温条件下,阴、阳生木芙蓉光合速率(Pn)、蒸腾速率(E)、胞间CO2浓度(Ci)、气孔导度(Gs)。结果表明,随着光强和温度的升高,阴、阳生木芙蓉的Pn、E逐渐升高,而Ci和Gs随光强和温度的的递增而递减。阳生木芙蓉的光合生理表现均优于阴生木芙蓉。本研究结果发现木芙蓉更适于在阳生环境下栽培,为木芙蓉的园林绿化提供技术参考。%With Hibiscus mutabilis in shade and sun as materials, response of gas-exchange parameters in Hibiscus mutabilis to light intensity and temperature were studied in this paper. The photosynthetic rate (Pn), transpiration rate (E), intercellular CO2 concentration (Ci), stomatal conductance (Gs) were determined. The results showed that with the increase of light intensity and temperature, Pn and E of Hibiscus mutabilis in shade and sun increased, while Ci and Gs decreased. Hibiscus mutabilis in the sun photosynthetic physiological performance were all superior to Hibiscusmutabilisin the shade. The results of this study found that Hibiscus mutabilis was more suitable for cultivation in the sun environment and provide technical reference for Hibiscusmutabilislandscaping.

  15. Influences of air pollution on the growth of ornamental tree species-particularly with reference to SO/sub 2/

    Energy Technology Data Exchange (ETDEWEB)

    Kim, T.W.

    1975-01-01

    For the purpose of detecting resistance to air pollution, particularly SO/sub 2/ contamination, six ornamental tree species were selected, i.e., Ginkgo biloba, Larix leptolepis, Pinus rigida, Syringa dilatata, Hibiscus syriacus, and Forsythia koreana. The sensitivity was observed and analyzed on the basis of the area ratio of smoke injury spot to the total leaf area. According to the results, the decreasing order of SO/sub 2/ sensitivity by species could be arranged as follows: (1) Hibiscus syriacus, (2) Ginkgo biloba, (3) Forsythia koreana, (4) Syringa dilatata, (5) Larix leptolepis, and (6) Pinus rigida. In general, Hibiscus syriacus and Ginkgo biloba can be grouped as the most resistant ones and Larix leptolepis and Pinus rigida as the weakest ones and Forsythia koreana and Syringa dilatata as the intermediate. Due to the sprouting ability and the formative ability of adventitious buds, the recovery from the SO/sub 2/ fumigation was prominent in Hibiscus syriacus, Syringa dilatata and Forsythia koreana. The differences in the smoke spot color were recognized by species, namely, dirt brown in Syringa dilatata, brilliant yellow brown in Pinus rigida and Ginkgo biloba, whitish yellow in Hibiscus syriacus, and red brown in Forsythia koreana. In the case of Ginkgo biloba and Larix leptolepis, the younger leaves were more resistant to SO/sub 2/ than the old ones. The sulfur content of leaves showed that on the basis of %/dry weight, broad-leaved species contained the higher amount of sulfur than the coniferous species. 15 ornamental tree species which have been growing in Seoul city were sampled from the 19 air polluted spots. The elucidated were the heavily polluted regions and the lightly polluted regions. The SO/sub 2/ absorbing capacities by species are explained in the text. 17 references.

  16. First report of Maconellicoccus hirsutus (Green, 1908) (Hemiptera: Coccoidea: Pseudococcidae) and the associated parasitoid Anagyrus kamali Moursi, 1948 (Hymenoptera: Encyrtidae), in Brazil.

    Science.gov (United States)

    Marsaro Júnior, A L; Peronti, A L B G; Penteado-Dias, A M; Morais, E G F; Pereira, P R V S

    2013-05-01

    The pink hibiscus mealybug (PHM), Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) and the associated hymenopterous parasitoid, Anagyrus kamali Moursi, 1948 (Hymenoptera: Encyrtidae), are reported for the first time in Brazil. Specimens of the PHM were collected on nine hosts plants, Annona muricata L. (Anonnaceae), Glycine max (L.) Merr. (Fabaceae), Centrolobium paraensis Tul. (Fabaceae), Inga edulis Mart. (Fabaceae), Hibiscus rosa-sinensis L. (Malvaceae), Psidium guajava L. (Myrtaceae), Averrhoa carambola L. (Oxalidaceae), Citrus sinensis (L.) Osbeck (Rutaceae) and Solanum lycopersicum L. (Solanaceae), in four municipalities in the north-northeast of the state of Roraima. The plants C. paraensis, I. edulis and C. sinensis are recorded for the first time as a hosts for PHM. Characteristic injuries observed on the host plants infested by PHM and suggestions for its management are presented.

  17. [The antiradical activity of plant extracts and healthful preventive combinations of these exrtacts with the phospholipid complex].

    Science.gov (United States)

    Baranova, V S; Rusina, I F; Guseva, D A; Prozorovskaia, N N; Ipatova, O M; Kasaikina, O T

    2012-01-01

    Using the chemiluminescence method, the effective concentration of antioxidants (AO) and its reactivity toward peroxyl radicals (ARA, the k7 constant) have been measured for 13 plant extracts. In fact all extracts demonstrated ARA higher than ionol. Larix dahurica, Hypericum perforatum, Potentilla fruticosa, Aronia melanocarpa and Rhaponticum carthamoides extracts showed the highest values of ARA. The combinations Aronia + Raponticum extracts; Larix + Hibiscus extracts; Schizandra +Aronia extracts were synergistic (the synergism effect beta of 38%, 33% and 22%). Apparently this phenomenon is the result of the synergistic interaction between compounds present in plant extracts. The Phospholipid complex--Lipoid S40, lacting any antioxidant effect alone, showed a potent synergistic effect with Aronia extract (beta3 = 60%), Silybum extract (beta3 = 41%). Clinical trials demonstrated, that combinations "Lipoid + Aronia extract", "Lipoid + Larix extract + Hibiscus extract", "Lipoid + Silybum extract", "Lipoid + Q10 + Rosa majalis extract" may be used as an additional component in the medicinal treatment, or as an individual prophylactic agent.

  18. EVALUATION OF ANTI-BACTERIAL ACTIVITY OF LOCAL FLORA OF BUNDELKHAND REGION OF JHANSI- INDIA AGAINST PLANT PATHOGENIC BACTERIA Xanthomonas campestris pv. campestris

    Directory of Open Access Journals (Sweden)

    Sazada Siddiqui

    2014-05-01

    Full Text Available Twenty plants namely Acacia nilotica (L. Willd.ex delil, Ageratum conyzoides Linn, Boerhaavia diffusa Linn., Cynodon dactylon (L. Pers, Cleome viscosa L, Datura stramonium Linn, Euphorbia hirta Linn, Ficus benghalensis Linn, Hyptis suaveolens (Linn poit, Hibiscus rosa-sinensis Linn, Jatropha gossypifolia Linn, Phyllanthus niruri webster, Prosopis juliflora, Polyalthia longifolia, Sida cordifolia, Tephrosia purpurea (Linn. Pers, Tridax procumbens Linn, Zizyphus jujube Linn, Solanum nigrum Linn, were collected from different localities and screened for their antibacterial activity against phytopathogenic bacterium, Xanthomonas campestris pv. campestris. Among all the tested species, nine plant species viz Acacia nilotica, Ageratum conyzoied, Boerhaavia diffusa, Cleome viscose, Datura stramonium, Euphorbia hirta, Hyptis suaveolens, Hibiscus rosa sinensis, Prosopis juliflora and Tridex procumbens showed medium to light antibacterial activity against the selected pathogens. Significant antibacterial activity was observed in aqueous extracts of Prosopsis juliflora, Hyptis suaveolens, Euphorbia hirta and Acacia nilotica

  19. Susceptibility pattern of Malassezia species to selected plant extracts and antifungal agents

    Directory of Open Access Journals (Sweden)

    G Sibi

    2014-01-01

    Full Text Available Objective: Malassezia is associated with dandruff, seborrhoeic dermatitis, pityriasis versicolor folliculitis and atopic eczema. This study determined the susceptibility pattern of Malassezia furfur, M. globosa, M. obtusa, M. restricta, M. slooffiae and M. sympodialis isolated from patients diagnosed with dandruff against plant extracts and antifungal agents. Materials and Methods: Twenty aqueous plant extracts and five azole drugs were tested against the isolates by well diffusion and broth dilution method. Results: Among the plant extracts, Phyllanthus emblica (fruits, Hibiscus rosa sinensis (flowers and Acacia concinna (pods have demonstrated significant antidandruff activity. Minimum inhibitory concentration values revealed that ketoconazole as the most effective drug followed by itraconazole. Conclusion: M. furfur and M. globosa were found as the most susceptible organisms against the aqueous extracts of Phyllanthus emblica (fruits, Hibiscus rosa sinensis (flowers, Acacia concinna (pods and azole drugs.

  20. Bleaching of the red anthocyanin induced by superoxide radical.

    Science.gov (United States)

    Yamasaki, H; Uefuji, H; Sakihama, Y

    1996-08-01

    Red anthocyanin prepared from petals of Hibiscus rosa-sinensis L. was photobleached in the EDTA-riboflavin system. The rate of bleaching monitored at 565 nm depended on the light intensity and EDTA concentrations. Anaerobic conditions and/or addition of superoxide dismutase prevented the bleaching of anthocyanin, whereas mannitol and catalase did not. A similar bleaching was observed under dark conditions in the xanthine-xanthine oxidase system. The results indicate that anthocyanin is bleached by the nonenzymatic reaction with the superoxide radical and suggest that the pigment can function as an antioxidant. The antioxidative efficiency of cyanidin to superoxide was 10-fold higher than that of cyanidin-3-sophoroside as a Hibiscus anthocyanin.

  1. A Comparative Study of Ultrasound Examination of Urinary Tract Performed on Spinal Cord Injury Patients with No Urinary Symptoms and Spinal Cord Injury Patients with Symptoms Related to Urinary Tract: Do Findings of Ultrasound Examination Lead to Changes in Clinical Management?

    Directory of Open Access Journals (Sweden)

    Subramanian Vaidyanathan

    2006-01-01

    Full Text Available Findings of ultrasound examination of the urinary tract and changes in clinical management, which were instituted on the basis of ultrasound examination, were compared between two groups of spinal cord injury patients. Group 1 had no urinary symptoms when they underwent the scan, whereas group 2 was comprised of patients with symptoms pertaining to the urinary tract. Between 2000 and 2006, ultrasound examination of the urinary tract was performed in 87 spinal cord injury patients who had no urinary symptoms when they underwent the ultrasound scan. No abnormality was found in 63 patients. The ultrasound scan showed some abnormality of the urinary tract in 24 patients (simple cyst in the kidney: 4; reduced size of a kidney: 3; increased echogenicity of left kidney: 1; prominent extrarenal pelvis and mild calyceal dilatation: 1; slightly dilated renal pelvis and calyceal system: 1; pelvic kidney showing mild hydronephrosis: 1; foetal lobulation of kidney: 2; multicystic kidney with no interval change in the appearance since last examination: 1; 2-cm-diameter parapelvic cyst: 1; small renal calyceal calculus: 5; a little cortical scarring bilaterally: 1; focal renal scar: 2; generalised thinning of renal cortex: 3; increase in renal sinus fat: 3; trabeculated bladder: 2; small vesical diverticulum: 1; mild generalised bladder wall thickening: 1; small residual urine in postvoid scan; 2. No specific interventions were performed in these patients on the basis of ultrasound findings. In Group 2, ultrasound examination revealed serious abnormalities such as hydronephrosis, pyonephrosis, vesical calculi, vesical polyp in 20 of 21 patients, and all 20 patients required therapeutic intervention on the basis of ultrasound scan findings.In conclusion, routine ultrasound examination of the urinary tract in spinal cord injury patients who have no urinary symptoms may not be justifiable in terms of cost effectiveness; limited hospital resources should be

  2. The euAP1 protein MPF3 represses MPF2 to specify floral calyx identity and displays crucial roles in Chinese lantern development in Physalis.

    Science.gov (United States)

    Zhao, Jing; Tian, Ying; Zhang, Ji-Si; Zhao, Man; Gong, Pichang; Riss, Simone; Saedler, Rainer; He, Chaoying

    2013-06-01

    The Chinese lantern phenotype or inflated calyx syndrome (ICS) is a postfloral morphological novelty in Physalis. Its origin is associated with the heterotopic expression of the MADS box gene 2 from Physalis floridana (MPF2) in floral organs, yet the process underlying its identity remains elusive. Here, we show that MPF3, which is expressed specifically in floral tissues, encodes a core eudicot APETALA1-like (euAP1) MADS-domain protein. MPF3 was primarily localized to the nucleus, and it interacted with MPF2 and some floral MADS-domain proteins to selectively bind the CC-A-rich-GG (CArG) boxes in the MPF2 promoter. Downregulating MPF3 resulted in a dramatic elevation in MPF2 in the calyces and androecium, leading to enlarged and leaf-like floral calyces; however, the postfloral lantern was smaller and deformed. Starch accumulation in pollen was blocked. MPF3 MPF2 double knockdowns showed normal floral calyces and more mature pollen than those found in plants in which either MPF3 or MPF2 was downregulated. Therefore, MPF3 specifies calyx identity and regulates ICS formation and male fertility through interactions with MPF2/MPF2. Furthermore, both genes were found to activate Physalis floridana invertase gene 4 homolog, which encodes an invertase cleaving Suc, a putative key gene in sugar partitioning. The novel role of the MPF3-MPF2 regulatory circuit in male fertility is integral to the origin of ICS. Our results shed light on the evolution and development of ICS in Physalis and on the functional evolution of euAP1s in angiosperms.

  3. The importance of hydronephrosis in percutaneous nephrolithotomy operation

    Directory of Open Access Journals (Sweden)

    Mehmet Murat Rifaioğlu

    2013-09-01

    Full Text Available Objective: The aim of this study is to investigate the relationbetween hydronephrosis grade and preoperative,peroperative and postoperative data and to evaluate theclinical significance of hydronephrosis in percutaneousnephrolithotomy (PNL.Methods: One hundred and thirty-two patients who hadundergone PNL operation by same surgeon betweenFebruary 2009 and February 2013 were reviewed retrospectively.Patients’ characteristics, preoperative kidneystatus, operative data were investigated. Grade 1, 2 hydronephrosiswere determined as Group I, Grade 3, 4hydronephrosis were determined as Group II. Preoperative,peroperative and postoperative property differencesbetween groups were compared statistically. For the multivariateanalysis, logistic regression analysis was usedto determine independent predictors of hydronephrosis.Results: Patients mean age was 46.2±13.7 years (8-73.Operation mean time was 101(10-250 minutes. 56 and76 patients’ data were analyzed in Group I and GroupII, respectively. There was no difference in age, gender,BMI, and surgeon experience between groups. Droppingstone history in same kidney (p=0.012, stone diameter(p=0.022, the number of calyceal stones (p=0.005, presencestone in the other kidney (p=0.027, renal parenchymalthickness (p=0.026 and operation time (p=0.011had significantly in univariate analysis. In logistic regressionmodel, dropping stone history in the same kidney,the number of calyceal stones, presence stone in theother kidney and operation time had a relationship withhydronephrosis.Conclusion: In PNL, patients with low-grade hydronephrosismight have decreased complication rates dueto diminished operation time, which resulted in reducednumber of calyceal stones and stone size.Key words: Percutaneous nephrolithotomy, PNL, hydronephrosis,outcome

  4. Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.

    Science.gov (United States)

    Vasileva, Mariya; Horstmann, Heinz; Geumann, Constanze; Gitler, Daniel; Kuner, Thomas

    2012-10-01

    Synapsins are abundant synaptic vesicle (SV)-associated proteins thought to mediate synaptic vesicle mobility and clustering at most synapses. We used synapsin triple knock-out (TKO) mice to examine the morphological and functional consequences of deleting all synapsin isoforms at the calyx of Held, a giant glutamatergic synapse located in the auditory brain stem. Quantitative three-dimensional (3D) immunohistochemistry of entire calyces showed lower amounts of the synaptic vesicle protein vGluT1 while the level of the active zone marker bassoon was unchanged in TKO terminals. Examination of brain lysates by ELISA revealed a strong reduction in abundance of several synaptic vesicle proteins, while proteins of the active zone cytomatrix or postsynaptic density were unaffected. Serial section scanning electron microscopy of large 3D-reconstructed segments confirmed a decrease in the number of SVs to approximately 50% in TKO calyces. Short-term depression tested at stimulus frequencies ranging from 10 to 300 Hz was accelerated only at frequencies above 100 Hz and the time course of recovery from depression was slowed in calyces lacking synapsins. These results reveal that in wild-type synapses, the synapsin-dependent reserve pool contributes to the replenishment of the readily releasable pool (RRP), although accounting only for a small fraction of the SVs that enter the RRP. In conclusion, our results suggest that synapsins may be required for normal synaptic vesicle biogenesis, trafficking and immobilization of synaptic vesicles, yet they are not essential for sustained high-frequency synaptic transmission at the calyx terminal.

  5. Comparison between the efficacy of dimeric and monomeric non-ionic contrast media (iodixanol vs iopromide) in urography in patients with macroscopic haematuria

    Energy Technology Data Exchange (ETDEWEB)

    Stacul, F.; Cova, M.; Pravato, M. [Department of Radiology, University of Trieste, Cattinara Hospital, Strada di Fiume, 447, 34149 Trieste (Italy); Floriani, I. [Istituto di Ricerche Farmacologiche Mario Negri, Milan (Italy)

    2003-04-01

    Non-ionic dimers induce less diuresis than non-ionic monomers, resulting in increased opacification of the urinary tract in intravenous urography. This trial compared the diagnostic efficacy of iodixanol and iopromide in patients with macroscopic haematuria. One hundred consecutive patients with normal renal function and macroscopic haematuria entered a double blind, comparative, randomised, parallel trial. Contrast media were given with bolus injection in doses of 300 mgI/kg b.w. Radiographs were blindly evaluated by three radiologists who analysed different parameters (calyceal density and filling, papillary blush detection, delineation of collecting ducts, renal pelvis opacification, visualisation of ureters, bladder density, bladder distention) and estimated the diagnostic confidence (whether abnormal findings were definitely absent, probably absent, doubtful, probably present or certainly present). Radiological diagnoses were compared with final diagnoses. Results were summarised as the ratio of the odds of having a worse performance of iopromide vs iodixanol. Iodixanol showed a significantly better calyceal density and filling [odds ratio (OR): 1.96; 95% confidence interval (CI): 1.60-2.41], a significantly better pelvis opacification (OR 2.91; CI 2.02-4.18) and a significantly more frequent papillary blush detection (OR 1.95; CI 1.29-2.95). Iopromide showed a significantly better ureteral visualisation (OR 0.67; CI 0.48-0.92) and a significantly higher bladder distention (OR 0.59; CI 0.36-0.99). Iodixanol allowed a significantly higher diagnostic confidence as to calyceal evaluation (OR 1.35; CI 1.01-1.79). No significant differences were found with regard to other parameters. The results confirmed theoretical expectations. The higher opacification provided by iodixanol allowed better results and a higher diagnostic confidence in the upper excretory pathway. (orig.)

  6. Nota sobre la presencia de Frankliniella insularis (Franklin) (Thysanoptera: thripidae) en la República Argentina

    OpenAIRE

    Rossa, F. R.; Bado, Silvina Graciela; Pannunzio, María Julia

    2004-01-01

    p.113-117 La presencia en la Argentina de Frankliniella insulares (Franklin) estaba cuestionada debido tanto a la imprecisión de las citas bibliográficas como al hallazgo de materiales coleccionados incorrectamente determinados. En recolecciones realizadas en 2002 y 2003 en Capital Federal, fueron hallados ejemplares de F. insularis sobre rosa china (Hibiscus sp.). Su novedosa presencia podría deberse a una reciente introducción probablemente favorecida por el comercio internacional de pla...

  7. Point Intercept and Surface Observation GPS (SOG): A Comparison of Survey Methods - Lake Gaston, NC/VA

    Science.gov (United States)

    2008-11-01

    coverage by hydrilla was greater than 50% (littoral zone approximately 5,000 acres, Madsen 2007). Scientific name Common name Exotic/native Whole lake...vegetation in the lake. Scientific name Common name Monospecific acres Total acres Native emergent Echinodorus cordifolius creeping burhead...0.001 0.001 Eleocharis macrostachya flatstem spikerush 0.002 0.002 E. quadrangulata squarestem spikerush 2.66 7.21 Hibiscus spp. mallow 0.06 0.27

  8. Bioavailability of thiamine, riboflavin and niacin from commonly consumed green leafy vegetables in the rural areas of Andhra Pradesh in India.

    Science.gov (United States)

    Girija, V; Sharada, D; Pushpamma, P

    1982-01-01

    The physiological availability of thiamine, riboflavin and niacin from three green leafy vegetables -- amaranth (Amaranthes gangeticus), gogu (Hibiscus cannabinus) and drumstick leaves (Moringa oleifera) was calculated from individual dose response curves. The bioavailability of thiamine and riboflavin was higher from drumstick leaves curry, while the availability of niacin was higher from gogu curry. But the differences in the bioavailability of the vitamins from the experimental curries was not significant.

  9. Interaction of Meloidogyne javanica and Macrophomina phaseoli in Kenaf Root Rot.

    Science.gov (United States)

    Tu, C C; Cheng, Y H

    1971-01-01

    Incidence and severity of root-rot caused by the fungus Macrophomina phaseoli was increased in screenhouse-grown kenaf (Hibiscus cannabinus L.) seedlings simultaneously infected by the nematode Meloidogyne javanica. In seedlings inoculated at 5, 10 and 15 days of age, root rot lesions increased 70.3, 44.1 and 21.8%, and nematode penetration increased 49.0, 36.7, and 12.3% when both fungus and nematode were present.

  10. Optimization and stabilization of gold nanoparticles by using herbal plant extract with microwave heating

    OpenAIRE

    Yasmin, Akbar; Ramesh, Kumaraswamy; Rajeshkumar, Shanmugam

    2014-01-01

    In this study, we have synthesized the gold nanoparticles by using Hibiscus rosa-sinensis, a medicinal plant. The gold nanoparticles were synthesized rapidly by the involvement of microwave heating. By changing of plant extract concentration, gold solution concentration, microwave heating time and power of microwave heating the optimized condition was identified. The surface Plasmon resonance found at 520 nm confirmed the gold nanoparticles synthesis. The spherical sized nanoparticles in the ...

  11. Kaempferol and Kaempferol Rhamnosides with Depigmenting and Anti-Inflammatory Properties

    OpenAIRE

    Jae Youl Cho; Dong Ha Cho; Keun Ha Lee; Sun Sang Kwon; Dae Sung Yoo; Soo Mi Ahn; Amal Kumar Ghimeray; Ho Sik Rho

    2011-01-01

    The objective of this study was to examine the biological activity of kaempferol and its rhamnosides. We isolated kaempferol (1), a-rhamnoisorobin (2), afzelin (3), and kaempferitrin (4) as pure compounds by far-infrared (FIR) irradiation of kenaf (Hibiscus cannabinus L.) leaves. The depigmenting and anti-inflammatory activity of the compounds was evaluated by analyzing their structure-activity relationships. The order of the inhibitory activity with regard to depigmentation and nitric oxide ...

  12. A preliminary survey of primitive crops cultivated in the northern Transvaal of South Africa

    OpenAIRE

    T. H. Arnold; K. J. Musil

    1983-01-01

    The different tribal economies of South Africa rely extensively on a number of primitive crop taxa which are cultivated as a primary food source. The most important of these include  Sorghum bicolor,  Pennisetum americanum, Citrullus lanatus, Lagenaria siceraria, Vigna unguiculata, Voandzeia subterranea and  Hibiscus esculentus. Morphological variation within these and a number of less important crops is discussed. The frequency with which each crop is grown and preference ratings allotted to...

  13. Investigation on Damage Caused by Adoretus sinicus Burmeister on Lotus (Nelumbo nucifera) and Other Crops in Hubei Province%湖北地区中华喙丽金龟对莲等作物的为害调查研究

    Institute of Scientific and Technical Information of China (English)

    王利丽; 刘义满; 孙亚林; 匡晶; 李双梅; 黄来春

    2015-01-01

    首次报道了中华喙丽金龟(Adoretus sinicus Burmeister)在湖北地区对莲(Nelumbo nucifera Gaertn.)、芋头[Colocasia esculenta (L.) Schoot]及黄秋葵(Hibiscus esulentus L.)的为害.调查研究了中华喙丽金龟的为害症状、为害程度及活动习性,并提出了防治措施.

  14. PROCESS VARIABLES OPTIMIZATION FOR PREPARATION AND CHARACTERIZATION OF NOVEL ADSORBENT FROM LIGNOCELLULOSIC WASTE

    OpenAIRE

    Zaira Zaman Chowdhury; Sharifuddin Mohd Zain; Rashid Atta Khan,; Arash Arami-Niya,; Khalisanni Khalid

    2012-01-01

    In this study, powdered activated carbon (PAC) was produced from agricultural waste biomass of kenaf (Hibiscus cannabinus L.) core or stalk, and the process variables were optimized by applying central composite design (CCD). The influences of carbonization temperature, duration of activation, and impregnation ratio on removal percentage of copper Cu(II) ions from aqueous solution and carbon yield were investigated. A quadratic model for adsorption percentage and a 2FI model for carbon yield ...

  15. Photosynthesis in a test tube- dye sensitized solar cells as a teaching tool

    Energy Technology Data Exchange (ETDEWEB)

    Raturi, Atul; Fepuleai, Yoheni [Division of Physics, School of Engineering and Physics, The University of the South Pacific, Suva (Fiji)

    2010-05-15

    Dye sensitized solar cells employing natural plant dyes as phosensitizers can be effectively used to train students in the science and technology of solar cells. This is especially relevant to developing countries where facilities for silicon cell fabrication are non-existent. The cross-disciplinary nature of this device makes it very attractive for student projects. The present work describes such a project where anthocyanin dye from hibiscus flowers has been used as the electron harvester. (author)

  16. Plica neuropathica causing traction alopecia

    Directory of Open Access Journals (Sweden)

    Pavithran K

    1990-01-01

    Full Text Available A middle aged woman developed matting of the hairs of the scalp following use of a home made shampoo that contained leaves of Hibiscus rosasiensis. The entangled and matted hair mass in the occipital region pulled hairs of the vertex region of the scalp, resulting in a patch of traction alopecia. Release of tension on the hairs by cutting them with scissors prevented further extension of alopecia.

  17. Radical scavenging potentials of single and combinatorial herbal formulations in vitro

    OpenAIRE

    2015-01-01

    Reactive oxygen and nitrogen species (RONS) are involved in deleterious/beneficial biological processes. The present study sought to investigate the capacity of single and combinatorial herbal formulations of Acanthus montanus, Emilia coccinea, Hibiscus rosasinensis, and Asystasia gangetica to act as superoxide radicals (SOR), hydrogen peroxide (HP), nitric oxide radical (NOR), hydroxyl radical (HR), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical antagonists using in vitro models. The herba...

  18. Dornier HM3 による上部尿路結石に対するESWL-1,000例の臨床的検討

    OpenAIRE

    江藤, 弘; 原田, 益善; 奥田, 喜啓; 前田, 浩志; 藤沢, 正人; 藤井, 明; 柯, 昭仁; 浜口, 毅樹; 郷司, 和男; 守殿, 貞夫

    1990-01-01

    Using a Dornier HM3 lithotripter, we treated 1,000 patients with renal and ureteral stones from April, 1986 to July, 1989. They consisted of 612 solitary stones (pelvic stones, 152; calyceal stones, 167; ureteral stones, 293), 265 multiple stones and 123 staghorn calculi (complete, 48; partial, 75). The overall rate of the auxiliary procedure was 59.3% (pre-operative, 47.9%; post-operative, 11.4%). Pre-operative procedure included 430 catheterizations, 26 percutaneous nephrostomies (PNSs) and...

  19. Nephrocutaneous fistula as the initial manifestation of asymptomatic nephrolithiasis: A call for radical management

    Directory of Open Access Journals (Sweden)

    Raman Tanwar

    2015-01-01

    Full Text Available Renal stones are a common affliction presenting in an acute setting. We report a case of asymptomatic renal stone in an elderly gentleman presenting initially as a discharging lumbar sinus managed by subcapsular nephrectomy and radical excision of the fistula tract. Nephrocutaneous fistula is most commonly associated with tuberculosis, xanthogranulomatous pyelonephritis, and rarely with complicated calyceal stones, and its occurrence with asymptomatic pelvic stones is rare. We present the points in favor of radical open surgery in the management of such patients.

  20. Mixed epithelial and stromal tumor of the kidney (MEST) simulating an upper tract TCC.

    Science.gov (United States)

    Sountoulides, Petros; Koptsis, Michail; Metaxa, Linda; Theodosiou, Alexandros; Kikidakis, Dimitrios; Filintatzi, Chrysa; Paschalidis, Konstantinos

    2012-02-01

    We present a rare and interesting case of a mixed epithelial and stromal tumour (MEST) of the kidney. The case is unique as it involves a male patient with no history of hormonal therapy presenting with a filling defect in the renal collecting system and positive urine cytology. The patient was diagnosed with transitional cell carcinoma of the renal pelvis and subjected to nephroureterectomy, which revealed a solid tumour arising from the lower calyces and extending into the renal pelvis and upper ureter. Pathology revealed a MEST. The patient was disease-free at the 6-month follow-up.

  1. The time of infection of apples by Botrytis cinerea Pers.

    OpenAIRE

    Hanna Bryk

    2013-01-01

    The time of infection of apple fruits by Botrytis cinerea Pers. was studied. Artificial inoculations with conidial suspensions of B. cinerea were done at different stages of fruit developmment (flowers, sets, fruits). In autumn the apples were harvested and stored at a temperature of 2°C for 4 months after which rotting caused by B. cinerea was evaluated. B. cinerea presence in the calyx of apples was checked throughout the growing season. This was done by plating flowers, apple and set calyc...

  2. Aphids (Hemiptera, Aphididae) on ornamental plants in greenhouses in Bulgaria.

    Science.gov (United States)

    Yovkova, Mariya; Petrović-Obradović, Olivera; Tasheva-Terzieva, Elena; Pencheva, Aneliya

    2013-01-01

    Investigations on the species composition and host range of aphids on ornamental greenhouse plants in Bulgaria was conducted over a period of five years, from 2008 to 2012. Twenty greenhouses, growing ornamentals for landscaping, plant collections and other purposes were observed. They were located in the regions of Sofia, Plovdiv, Smolyan, Pavlikeni, Varna and Burgas. The total number of collected aphid samples was 279. Their composition included 33 aphid species and one subspecies from 13 genera and 5 subfamilies. Twenty-eight species were found to belong to subfamily Aphidinae. Almost 70 % of all recorded species were polyphagous. The most widespread aphid species was Myzus persicae, detected in 13 greenhouses all year round, followed by Aulacorthum solani (10 greenhouses) and Aphis gossypii (9 greenhouses). The widest host range was shown by Myzus persicae (43 hosts), Aulacorthum solani (32 hosts) and Aulacorthum circumflexum (23 hosts). The list of host plants includes 114 species from 95 genera and 58 families. The greatest variety of aphid species was detected on Hibiscus (9 species). Out of all aphid samples 12.9 % were collected on Hibiscus and 6.8 %, on Dendranthema. The greatest variety of aphid species was detected on Hibiscus (9 species). Periphyllus californiensis and Aphis (Aphis) fabae mordvilkoi are reported for the first time for Bulgaria. Furthermore, Aphis spiraecola has been found in new localities and has widened its host range in this country.

  3. Life history of Paracoccus marginatus (Hemiptera: Pseudococcidae) on four host plant species under laboratory conditions.

    Science.gov (United States)

    Amarasekare, Kaushalya G; Mannion, Catharine M; Osborne, Lance S; Epsky, Nancy D

    2008-06-01

    Life history of the mealybug, Paracoccus marginatus Williams and Granara de Willink, on three ornamental plants [Hibiscus rosa-sinensis L., Acalypha wilkesiana (Muell.-Arg.), and Plumeria rubra L.] and one weed species (Parthenium hysterophorus L.) was studied under laboratory conditions. Mealybugs were able to develop, survive, and reproduce on all four hosts; however, there were differences in the life history parameters. Adult females that developed on acalypha and parthenium emerged approximately 1 d earlier than those that developed on hibiscus and plumeria. Adult males had a longer developmental time on plumeria than on the other hosts. Survival of first- and second-instar nymphs and cumulative adult survival were lowest on plumeria. Longevity was not affected by hosts for males and females and averaged 2.3 +/- 0.1 and 21.2 +/- 0.1 d, respectively. On plumeria, 58.9 +/- 1.7% of the adults were females, which was a higher female percentage than on the other hosts. No egg production occurred in virgin females. Prereproductive and reproductive periods of the females were not affected by hosts and averaged 6.3 +/- 0.1 and 11.2 +/- 0.1 d, respectively. Mean fecundity of 186.3 +/- 1.8 eggs on plumeria was lower than on the other three plant species. Life history parameters of P. marginatus on hibiscus, acalypha, plumeria, and parthenium show its ability to develop, survive, and reproduce on a wide variety of plant species.

  4. Survival and preference of cotton boll weevil adults for alternative food sources

    Directory of Open Access Journals (Sweden)

    M. Pimenta

    Full Text Available Abstract Plants that have potential as alternative food source (floral nectar, pollen and plant tissues to the boll weevil during the intercropping season were evaluated considering the prevalent conditions of Cerrado in the Central Brazil. Initially, we tested the nutritional adequacy for the survival of the insect of flower resource (pollen and nectar provided by eight plant species (fennel, mexican sunflower, castor bean, okra, hibiscus, sorghum, pigeonpea and sunn hemp. Subsequently, we tested if the resources provided by the selected plants continued to be exploited by the boll weevil in the presence of cotton plant, its main food source average longevity of boll weevil adults was significantly longer when they were fed on hibiscus’ flowers (166.6 ± 74.4 and okra flowers (34.7 ± 28.9 than when they fed on flowers of other six species. Subsequently, the preference of the boll weevil in the use of resources was compared between okra or hibiscus and cotton plants, in dual choice experiments. Boll weevils preferred plants of the three species in the reproductive stages than those in vegetative stages. Although the cotton plant in the reproductive stage was the most preferred plant of all, boll weevils preferred flowering okra and hibiscus than cotton at the vegetative stage.

  5. Percutaneous nephrolithotomy with and without retrograde pyelography: a randomized clinical trial

    Directory of Open Access Journals (Sweden)

    Ali Tabibi

    2007-02-01

    Full Text Available OBJECTIVE: Since the introduction of percutaneous nephrolithotomy (PCNL, many changes have been added regarding the entrance to pyelocalyceal system such as insertion of the needle pointed to an opaque stone as a guided landmark. We aim at comparing the outcomes of managing renal calculi with and without retrograde pyelography. MATERIALS AND METHODS: In a randomized clinical trial, 55 cases with opaque renal calculi candidates for PCNL with stone in one calyce, in the pelvis or both in one calyce and the pelvis simultaneously were included in a nine-month study. They were randomized into 2 groups, noncatheterized (n = 28 and catheterized (n = 27, called intervention and control groups, respectively. RESULTS: The 2 groups had similar distributions regarding gender, age, duration of operation, length of hospital stay, past history of any surgical procedures on kidney, and stone size. Outcome (residual stone based on aforementioned management was evaluated with plain X-ray on the morning following the operation. Postoperative hemoglobin decrease was significantly higher in controls than in the intervention group (p < 0.001 (with no clinical significance. No difference in outcome, postoperative fever, duration of surgery, duration of hospital stay and radiation exposure was observed between the 2 groups. CONCLUSION: Our findings showed no differences in major clinical outcomes between the 2 groups (with and without catheter insertion for retrograde pyelography.

  6. Risks and benefits of the intercostal approach for percutaneous nephrolithotripsy

    Directory of Open Access Journals (Sweden)

    Erich K. Lang

    2009-06-01

    Full Text Available OBJECTIVE:The objective of our retrospective study was to provide evidence on the efficacy of the intercostal versus subcostal access route for percutaneous nephrolithotripsy. MATERIAL AND METHODS: 642 patients underwent nephrolithotomy or nephrolithotripsy from 1996 to 2005. A total of 127 had an intercostal access tract (11th or 12th; 515 had a subcostal access tract. RESULTS: Major complications included one pneumothorax (1.0%, one arterio-calyceal fistula (1.0% and three arteriovenous fistulae (2.7% for intercostal upper pole access; two pneumothoraces (1.7%, one arteriovenous fistula (1.0%, one pseudoaneurysm (1.0%, one ruptured uretero-pelvic junction (1.0%, 4 perforated ureters (3.4% for subcostal upper pole access; one hemothorax (1.6%, one colo-calyceal fistula (1.6%, one AV fistula (1.6%, and two perforated ureters (3.2% with subcostal interpolar access. Diffuse bleeding from the tract with a subcostal interpolar approach occurred 3.2% of the time compared with 2.4% with a lower pole approach. Staghorn calculi demonstrated similar rates of complications. CONCLUSION: Considering the advantages that the intercostal access route offers the surgeon, it is reasonable to recommend its use after proper pre-procedural assessment of the anatomy, and particularly the respiratory lung motion.

  7. Preliminary clinical trial in percutaneous nephrolithotomy using a real-time navigation system for percutaneous kidney access

    Science.gov (United States)

    Rodrigues, Pedro L.; Moreira, António H. J.; Rodrigues, Nuno F.; Pinho, A. C. M.; Fonseca, Jaime C.; Lima, Estevão.; Vilaça, João. L.

    2014-03-01

    Background: Precise needle puncture of renal calyces is a challenging and essential step for successful percutaneous nephrolithotomy. This work tests and evaluates, through a clinical trial, a real-time navigation system to plan and guide percutaneous kidney puncture. Methods: A novel system, entitled i3DPuncture, was developed to aid surgeons in establishing the desired puncture site and the best virtual puncture trajectory, by gathering and processing data from a tracked needle with optical passive markers. In order to navigate and superimpose the needle to a preoperative volume, the patient, 3D image data and tracker system were previously registered intraoperatively using seven points that were strategically chosen based on rigid bone structures and nearby kidney area. In addition, relevant anatomical structures for surgical navigation were automatically segmented using a multi-organ segmentation algorithm that clusters volumes based on statistical properties and minimum description length criterion. For each cluster, a rendering transfer function enhanced the visualization of different organs and surrounding tissues. Results: One puncture attempt was sufficient to achieve a successful kidney puncture. The puncture took 265 seconds, and 32 seconds were necessary to plan the puncture trajectory. The virtual puncture path was followed correctively until the needle tip reached the desired kidney calyceal. Conclusions: This new solution provided spatial information regarding the needle inside the body and the possibility to visualize surrounding organs. It may offer a promising and innovative solution for percutaneous punctures.

  8. 3D excretory MR urography: improved image quality with intravenous saline and diuretic administration.

    Science.gov (United States)

    Ergen, F Bilge; Hussain, Hero K; Carlos, Ruth C; Johnson, Timothy D; Adusumilli, Saroja; Weadock, William J; Korobkin, Melvyn; Francis, Isaac R

    2007-04-01

    To assess the effect of diuretic administration on the image quality of excretory magnetic resonance urography (MRU) obtained following intravenous hydration, and to determine whether intravenous hydration alone is sufficient to produce diagnostic quality studies of nondilated upper tracts. A total of 22 patients with nondilated upper tracts were evaluated with contrast-enhanced MRU. All patients received 250 mL of saline intravenously immediately prior to the examination. A total of 11 patients received 10-20 mg furosemide in addition to saline. Imaging was performed with a three-dimensional (3D) and two-dimensional (2D) breathhold spoiled gradient-echo sequences. Excretory MRU images were acquired five minutes after the administration of 0.1 mmol/kg gadolinium and were independently reviewed by two radiologists, who were blinded to the MRU technique. Readers evaluated the calyces, renal pelvis, and ureters qualitatively for degree of opacification, distention, and artifacts on a four-point scale. Statistical analysis was performed using a permutation test. There was no significant disagreement between the two readers (P=0.14). Furosemide resulted in significant improvement in calyceal and renal pelvis distention (PMRU studies obtained following intravenous hydration. Intravenous saline alone is insufficient to produce diagnostic quality studies of the non-dilated upper tracts. Copyright (c) 2007 Wiley-Liss, Inc.

  9. Gentamicin induced nitric oxide-related oxidative damages on vestibular afferents in the guinea pig.

    Science.gov (United States)

    Hong, Sung Hwa; Park, Sook Kyung; Cho, Yang-Sun; Lee, Hyun-Seok; Kim, Ki Ryung; Kim, Myung Gu; Chung, Won-Ho

    2006-01-01

    Gentamicin is a well-known ototoxic aminoglycoside. However, the mechanism underlying this ototoxicity remains unclear. One of the mechanisms which may be responsible for this ototoxicity is excitotoxic damage to hair cells. The overstimulation of the N-methyl-d-aspartate (NMDA) receptors increases the production of nitric oxide (NO), which induces oxidative stress on hair cells. In order to determine the mechanism underlying this excitotoxicity, we treated guinea pigs with gentamicin by placing gentamicin (0.5 mg) pellets into a round window niche. After the sacrifice of the animals, which occurred at 3, 7 and 14 days after the treatment, the numbers of hair cells in the animals were counted with a scanning electron microscope. We then performed immunostaining using neuronal nitric oxide synthase (nNOS), inducible NOS (iNOS) and nitrotyrosine antibodies. The number of hair cells in the animals was found to decrease significantly after 7 days. nNOS and iNOS expression levels were observed to have increased 3 days after treatment. Nitrotyrosine was expressed primarily at the calyceal afferents of the type I hair cells 3 days after treatment. Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) staining revealed positive hair cells 3 days after treatment. Our results suggest that inner ear treatment with gentamicin may upregulate nNOS and iNOS to induce oxidative stress in the calyceal afferents of type I hair cells, via nitric oxide overproduction.

  10. Neural substrate for higher-order learning in an insect: Mushroom bodies are necessary for configural discriminations.

    Science.gov (United States)

    Devaud, Jean-Marc; Papouin, Thomas; Carcaud, Julie; Sandoz, Jean-Christophe; Grünewald, Bernd; Giurfa, Martin

    2015-10-27

    Learning theories distinguish elemental from configural learning based on their different complexity. Although the former relies on simple and unambiguous links between the learned events, the latter deals with ambiguous discriminations in which conjunctive representations of events are learned as being different from their elements. In mammals, configural learning is mediated by brain areas that are either dispensable or partially involved in elemental learning. We studied whether the insect brain follows the same principles and addressed this question in the honey bee, the only insect in which configural learning has been demonstrated. We used a combination of conditioning protocols, disruption of neural activity, and optophysiological recording of olfactory circuits in the bee brain to determine whether mushroom bodies (MBs), brain structures that are essential for memory storage and retrieval, are equally necessary for configural and elemental olfactory learning. We show that bees with anesthetized MBs distinguish odors and learn elemental olfactory discriminations but not configural ones, such as positive and negative patterning. Inhibition of GABAergic signaling in the MB calyces, but not in the lobes, impairs patterning discrimination, thus suggesting a requirement of GABAergic feedback neurons from the lobes to the calyces for nonelemental learning. These results uncover a previously unidentified role for MBs besides memory storage and retrieval: namely, their implication in the acquisition of ambiguous discrimination problems. Thus, in insects as in mammals, specific brain regions are recruited when the ambiguity of learning tasks increases, a fact that reveals similarities in the neural processes underlying the elucidation of ambiguous tasks across species.

  11. Dynamic control of synaptic vesicle replenishment and short-term plasticity by Ca(2+)-calmodulin-Munc13-1 signaling.

    Science.gov (United States)

    Lipstein, Noa; Sakaba, Takeshi; Cooper, Benjamin H; Lin, Kun-Han; Strenzke, Nicola; Ashery, Uri; Rhee, Jeong-Seop; Taschenberger, Holger; Neher, Erwin; Brose, Nils

    2013-07-10

    Short-term synaptic plasticity, the dynamic alteration of synaptic strength during high-frequency activity, is a fundamental characteristic of all synapses. At the calyx of Held, repetitive activity eventually results in short-term synaptic depression, which is in part due to the gradual exhaustion of releasable synaptic vesicles. This is counterbalanced by Ca(2+)-dependent vesicle replenishment, but the molecular mechanisms of this replenishment are largely unknown. We studied calyces of Held in knockin mice that express a Ca(2+)-Calmodulin insensitive Munc13-1(W464R) variant of the synaptic vesicle priming protein Munc13-1. Calyces of these mice exhibit a slower rate of synaptic vesicle replenishment, aberrant short-term depression and reduced recovery from synaptic depression after high-frequency stimulation. Our data establish Munc13-1 as a major presynaptic target of Ca(2+)-Calmodulin signaling and show that the Ca(2+)-Calmodulin-Munc13-1 complex is a pivotal component of the molecular machinery that determines short-term synaptic plasticity characteristics.

  12. Diuretic Agent and Normal Saline Infusion Technique for Ultrasound-Guided Percutaneous Nephrostomies in Nondilated Pelvicaliceal Systems

    Energy Technology Data Exchange (ETDEWEB)

    Yagci, Cemil, E-mail: cemil.yagci@medicine.ankara.edu.tr; Ustuner, Evren, E-mail: evrenustuner@hotmail.com; Atman, Ebru Dusunceli, E-mail: ebrumd2001@yahoo.com [Ankara University, Department of Radiology, School of Medicine (Turkey); Baltaci, Sumer, E-mail: sbaltaci@hotmail.com [Ankara University, Department of Urology, School of Medicine (Turkey); Uzun, Caglar, E-mail: cuzun77@yahoo.com; Akyar, Serdar, E-mail: yusuf.s.akyar@medicine.ankara.edu.tr [Ankara University, Department of Radiology, School of Medicine (Turkey)

    2013-04-15

    Percutaneous nephrostomy (PCN) in a nondilated pelvicaliceal system is technically challenging. We describe an effective method to achieve transient dilatation of the pelvicaliceal system via induction of diuresis using infusion of a diuretic agent in normal saline, therefore allowing easier access to the pelvicaliceal system. Under real-time ultrasound guidance, the technique had been tested in 22 nephrostomies with nondilated system (a total of 20 patients with 2 patients having bilateral nephrostomies) during a 5-year period. Patients were given 40 mg of furosemide in 250 ml of normal saline solution intravenously by rapid infusion. As soon as maximum calyceal dilatation of more than 5 mm was observed, which is usually 15 min later after the end of rapid infusion, patients were positioned obliquely, and PCN procedure under ultrasound guidance was performed. The procedure was successful in 19 of the nephrostomies in 17 patients with a success rate of 86.36 % per procedure and 85 % per patient in nondilated pelvicaliceal systems. No major nephrostomy-, drug-, or technique-related complications were encountered. The technique failed to work in three patients due to the presence of double J catheters and preexisting calyceal perforation which avoided transient dilation of the pelvicaliceal system with diuresis. Diuretic infusion in saline is a feasible and effective method for PCN in nondilated pelvicaliceal systems.

  13. Bilateral uric acid nephrolithiasis and ureteral hypertrophy in a free-ranging river otter (Lontra canadensis)

    Science.gov (United States)

    Grove, Robert A.; Bildfell, Rob; Henny, Charles J.; Buhler, D.R.

    2003-01-01

    We report the first case of uric acid nephrolithiasis in a free-ranging river otter (Lontra canadensis). A 7 yr old male river otter collected from the Skagit River of western Washington (USA) had bilateral nephrolithiasis and severely enlarged ureters (one of 305 examined [0.33%]). The uroliths were 97% uric acid and 3% protein. Microscopic changes in the kidney were confined to expansion of renal calyces, minor loss of medullary tissue, and multifocal atrophy of the cortical tubules. No inflammation was observed in either kidney or the ureters. The ureters were enlarged due to marked hypertrophy of smooth muscle plus dilation of the lumen. Fusion of the major calyces into a single ureteral lumen was several cm distal to that of two adult male otters used as histopathologic control specimens. This case report is part of a large contaminant study of river otters collected from Oregon and Washington. It is important to understand diseases and lesions of the otter as part of our overall evaluation of this population.

  14. Investment in higher order central processing regions is not constrained by brain size in social insects.

    Science.gov (United States)

    Muscedere, Mario L; Gronenberg, Wulfila; Moreau, Corrie S; Traniello, James F A

    2014-06-07

    The extent to which size constrains the evolution of brain organization and the genesis of complex behaviour is a central, unanswered question in evolutionary neuroscience. Advanced cognition has long been linked to the expansion of specific brain compartments, such as the neocortex in vertebrates and the mushroom bodies in insects. Scaling constraints that limit the size of these brain regions in small animals may therefore be particularly significant to behavioural evolution. Recent findings from studies of paper wasps suggest miniaturization constrains the size of central sensory processing brain centres (mushroom body calyces) in favour of peripheral, sensory input centres (antennal and optic lobes). We tested the generality of this hypothesis in diverse eusocial hymenopteran species (ants, bees and wasps) exhibiting striking variation in body size and thus brain size. Combining multiple neuroanatomical datasets from these three taxa, we found no universal size constraint on brain organization within or among species. In fact, small-bodied ants with miniscule brains had mushroom body calyces proportionally as large as or larger than those of wasps and bees with brains orders of magnitude larger. Our comparative analyses suggest that brain organization in ants is shaped more by natural selection imposed by visual demands than intrinsic design limitations.

  15. Evidence of a sensory processing unit in the mammalian macula

    Science.gov (United States)

    Chimento, T. C.; Ross, M. D.

    1996-01-01

    We cut serial sections through the medial part of the rat vestibular macula for transmission electron microscopic (TEM) examination, computer-assisted 3-D reconstruction, and compartmental modeling. The ultrastructural research showed that many primary vestibular neurons have an unmyelinated segment, often branched, that extends between the heminode (putative site of the spike initiation zone) and the expanded terminal(s) (calyx, calyces). These segments, termed the neuron branches, and the calyces frequently have spine-like processes of various dimensions with bouton endings that morphologically are afferent, efferent, or reciprocal to other macular neural elements. The major questions posed by this study were whether small details of morphology, such as the size and location of neuronal processes or synapses, could influence the output of a vestibular afferent, and whether a knowledge of morphological details could guide the selection of values for simulation parameters. The conclusions from our simulations are (1) values of 5.0 k omega cm2 for membrane resistivity and 1.0 nS for synaptic conductance yield simulations that best match published physiological results; (2) process morphology has little effect on orthodromic spread of depolarization from the head (bouton) to the spike initiation zone (SIZ); (3) process morphology has no effect on antidromic spread of depolarization to the process head; (4) synapses do not sum linearly; (5) synapses are electrically close to the SIZ; and (6) all whole-cell simulations should be run with an active SIZ.

  16. Dynamic Renal Scintigraphy in Diagnosis of Upper Urinary Tract Obstruction in Transplanted Kidney

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Nuclide renal dynamic imaging was performed on 88 (110 times) transplanted kidney. Two kinds of renal scintigraphic characteristics were identified in recipients with supravesical obstruction of the graft. First, the regular type was characterized by radioactivity defect area in kidney parenchyma during early uptake period followed by ureteropelvic retention. Second, the tubular type was typified by cortical retention and attenuation in collecting system during the whole test period with a special sign of “hollow kidney”. Non-obstructive dilated calyces showed similar signs as the regular type. Acute rejection reaction and tubule necrosis demonstrated obstructive time-activity curves. However, the radioactivity retention appeared in cortex. It was suggested that dilated calyces and obstructive renogram might not be reliable evidence for upper urinary tract obstruction. The signs of radioactivity attenuation in kidney parenchyma during early uptake period followed by ureteropelvic retention may be more valuable for the evaluation. As for tubular obstruction, specified “hollow kidney” was the characteristic sign which is helpful for the diagnosis.

  17. Pancake kidney with a single ureter: a rare incidental observation at autopsy.

    Science.gov (United States)

    Kanchan, Tanuj; Murlimanju, B V; Saralaya, Vasudha V

    2017-01-01

    We report an extremely rare case of a pancake kidney with a single ureter. During the medicolegal autopsy on the body of a traffic accident victim, the kidneys were not located in the abdomen. The anterior surfaces of both kidneys were completely fused in the pelvic cavity, and the kidney was shaped like a pancake. This pancake kidney had accessory renal arteries around it and one renal vein from each kidney. The left renal vein was much smaller in diameter than the right one. There were two major calyces, one each from each kidney. The major calyces joined to form a single renal pelvis from both the kidneys, which continued as a single ureter. The renal pelvis and ureter lay posterior to the pancake kidney. Urologists and pelvic surgeons should be aware of the variant anatomy of the pancake kidney, particularly concerning its vasculature, to prevent unexpected catastrophic bleeding. If the kidneys are not located in the abdominal cavity, the autopsy surgeon must consider the possibility of its presence in the pelvic cavity. This could be especially important during medicolegal investigations into allegations of missing kidneys and unlawful kidney transplantations.

  18. Serotonin-immunoreactive neurons in the antennal sensory system of the brain in the carpenter ant, Camponotus japonicus.

    Science.gov (United States)

    Tsuji, Eriko; Aonuma, Hitoshi; Yokohari, Fumio; Nishikawa, Michiko

    2007-08-01

    Social Hymenoptera such as ants or honeybees are known for their extensive behavioral repertories and plasticity. Neurons containing biogenic amines appear to play a major role in controlling behavioral plasticity in these insects. Here we describe the morphology of prominent serotonin-immunoreactive neurons of the antennal sensory system in the brain of an ant, Camponotus japonicus. Immunoreactive fibers were distributed throughout the brain and the subesophageal ganglion (SOG). The complete profile of a calycal input neuron was identified. The soma and dendritic elements are contralaterally located in the lateral protocerebrum. The neuron supplies varicose axon terminals in the lip regions of the calyces of the mushroom body, axon collaterals in the basal ring but not in the collar region, and other axon terminals ipsilaterally in the lateral protocerebrum. A giant neuron innervating the antennal lobe has varicose axon terminals in most of 300 glomeruli in the ventral region of the antennal lobe (AL) and a thick neurite that spans the entire SOG and continues towards the thoracic ganglia. However, neither a soma nor a dendritic element of this neuron was found in the brain or the SOG. A deutocerebral projection neuron has a soma in the lateral cell-body group of the AL, neuronal branches at most of the 12 glomeruli in the dorsocentral region of the ipsilateral AL, and varicose terminal arborizations in both hemispheres of the protocerebrum. Based on the present results, tentative subdivisions in neuropils related to the antennal sensory system of the ant brain are discussed.

  19. Efficacy of Percutaneous Nephrolithotomy in Patients with Neurogenic Bladder Dysfunction

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    Abdulkadir Tepeler

    2010-09-01

    Full Text Available Aim: Patients with neurogenic bladder dysfunction (NBD are at increased risk of urolithiasis. We review our experience with percutaneous nephrolithotomy (PCNL on this group of patients with special attention paid to the risks of surgical complications. Methods: A total of 18 cases with NBD underwent PCNL at our institution. Neurogenous lesions included traumatic spinal cord injuries in 8 cases, sequel of cerebrovascular accident in 2, meningitis in 2, polio in 4 and kyphoscoliosis in 2. Percutaneous access was achieved under fluoroscopic guidance with a patient in the prone position and tract was formed using balloon dilation system. Stone disintegration was accomplished with a pneumatic lithotripter. Results: There were 8 staghorn stones, 6 pelvi-calyceal stones, and 4 isolated renal pelvic stones. The mean operation time was 97.5±18.7 (range: 80-120 minutes and the mean hospitalization time was 3 (range: 2-5 days. Stone-free status was achieved in 13 (72.2% renal units. 4 (22% patients with 2 staghorn stones and 2 pelvi-calyceal stones had clinically insignificant residual fragments (CIRF. Hydropneumothorax was encountered in 1 case with kyphoscoliosis, in whom an intercostal access was necessary. Conclusion: PCNL in patients with NBD is safe and effective, with outcomes comparable to that of patients without such a disorder. (The Medical Bulletin of Haseki 2010; 48: 76-9

  20. Renal Pelviceal Keratinizing Squamous Metaplasia with Sparing of Pyramidal Zones

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    Richard H. Siderits

    2012-01-01

    Full Text Available Metaplastic changes in the urothelium of the upper urinary tract are relatively infrequent. Metaplasia may present as either squamous or less often glandular differentiation. The process may be associated with chronic inflammation or associated chronic infections. There may be malignant transformation to either squamous cell carcinoma or adenocarcinoma. The demarcation of the metaplastic process in the minor calyces has not been well documented to date. We report the case of a 74-year-old female patient who presented with a history of chronic renal disease and acute pyohydronephrosis. The patient underwent a nephroureterectomy which revealed keratinizing desquamative squamous metaplasia throughout the renal pelvis and upper urinary tract with abrupt termination of metaplasia at the junction of the renal pelvis and the minor calyx (pyramidal zone. Immunohistochemical evaluation documents metaplastic urothelium stained positive for CK5, before converting sharply to simple cuboidal epithelium in the minor calyx (pyramidal zones which stained positive CK7. At the junction of the metaplastic components and low cuboidal lined minor calyceal surfaces, the underlying stroma showed loss of ureteral muscularis mucosa with transition to renal parenchymal type stroma. We believe that this observation is unique and potentially relevant to the etiology and pathophysiology of pelviceal metaplasia.

  1. The Circuitry of Olfactory Projection Neurons in the Brain of the Honeybee, Apis mellifera

    Science.gov (United States)

    Zwaka, Hanna; Münch, Daniel; Manz, Gisela; Menzel, Randolf; Rybak, Jürgen

    2016-01-01

    In the honeybee brain, two prominent tracts – the medial and the lateral antennal lobe tract – project from the primary olfactory center, the antennal lobes (ALs), to the central brain, the mushroom bodies (MBs), and the protocerebral lobe (PL). Intracellularly stained uniglomerular projection neurons were reconstructed, registered to the 3D honeybee standard brain atlas, and then used to derive the spatial properties and quantitative morphology of the neurons of both tracts. We evaluated putative synaptic contacts of projection neurons (PNs) using confocal microscopy. Analysis of the patterns of axon terminals revealed a domain-like innervation within the MB lip neuropil. PNs of the lateral tract arborized more sparsely within the lips and exhibited fewer synaptic boutons, while medial tract neurons occupied broader regions in the MB calyces and the PL. Our data show that uPNs from the medial and lateral tract innervate both the core and the cortex of the ipsilateral MB lip but differ in their innervation patterns in these regions. In the mushroombody neuropil collar we found evidence for ALT boutons suggesting the collar as a multi modal input site including olfactory input similar to lip and basal ring. In addition, our data support the conclusion drawn in previous studies that reciprocal synapses exist between PNs, octopaminergic-, and GABAergic cells in the MB calyces. For the first time, we found evidence for connections between both tracts within the AL. PMID:27746723

  2. A revision of the genus Muricea Lamouroux, 1821 (Anthozoa, Octocorallia) in the eastern Pacific. Part II.

    Science.gov (United States)

    Breedy, Odalisca; Guzman, Hector M

    2016-01-01

    The species of the genus Muricea were mainly described from 1846 to 1870. After that very few contributions were published. Although the highest richness of Muricea species is in the eastern Pacific shallow waters, a comprehensive systematic study of the genus does not exist. Recently we started a taxonomic review of the genus in order to validate the status of four species previously included in the genus Eumuricea. Herein we present the second part of the Muricea revision dealing with the species-group characterised by shelf-like calyces instead of tubular-like calyces (the Muricea squarrosa-group). Original type material was morphologically analysed and illustrated using optical and scanning electron microscopy. Comparative character tables are provided for the genus. The taxonomic status of the species was analysed and established by designating lectotypes, alternatively by recognising a holotype by monotypy. We conclude that the genus Muricea comprises 20 valid species, including the previous four in the Muricea squarrosa-group. We propose 10 lectotypes, a new combination and three more species groups for the genus based on morphology: the Muricea fruticosa-group, Muricea plantaginea-group and Muricea austera-group.

  3. The circuitry of olfactory projection neurons in the brain of the honeybee, Apis mellifera

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    Hanna Zwaka

    2016-09-01

    Full Text Available In the honeybee brain, two prominent tracts - the medial and the lateral antennal lobe tract - project from the primary olfactory center, the antennal lobes, to the central brain, the mushroom bodies, and the protocerebral lobe. Intracellularly stained uniglomerular projection neurons (uPN were reconstructed, registered to the 3D honeybee standard brain atlas, and then used to derive the spatial properties and quantitative morphology of the neurons of both tracts. We evaluated putative synaptic contacts of projection neurons using confocal microscopy. Analysis of the patterns of axon terminals revealed a domain-like innervation within the mushroom body lip neuropil. Projection neurons of the lateral tract arborized more sparsely within the lips and exhibited fewer synaptic boutons, while medial tract neurons occupied broader regions in the mushroom body calyces and the protocerebral lobe. Our data show that uPNs from the medial and lateral tract innervate both the core and the cortex of the ipsilateral mushroom body lip but differ in their innervation patterns in these regions. In the mushroombody neuropil collar we found evidence for ALT boutons suggesting the collar as a multi modal input site including olfactory input similar to lip and basal ring. In addition, our data support the conclusion drawn in previous studies that reciprocal synapses exist between projection neurons, octopaminergic-, and GABAergic cells in the mushroom body calyces. For the first time, we found evidence for connections between both tracts within the antennal lobe.

  4. Robotic transmesocolonic Pyelolithotomy of horseshoe kidney

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    Emad S Rajih

    2015-02-01

    Full Text Available Introduction The purpose of this video is to demonstrate the use of the robot to perform a transmesocolonic pyelolithotomy of a horseshoe kidney. Materials and Methods A 35-year old female presented with vague abdominal pain. CT scan imaging revealed the presence of a left horseshoe kidney with multiple pelvicalyceal stones. The patient was positioned in the supine position. A total of 4 ports were introduced. A 3-arm da Vinci robotic surgical system was docked, and the arms were connected. First, the dilated renal pelvis was identified behind the thin mesocolon. The mesocolon was entered and renal pelvis was dissected completely from the surrounding fat. Then, the renal pelvis was opened after adequate dissection and stones were visualized inside the calyces. By Prograsp forceps, stones were removed from all the calyces under vision and were extracted from the assistant trocar. Finally, the pylotomy incision was closed using 4 0 Maxon in a continuous fashion and the mesocolon was closed using 3 0 PDS interrupted sutures. A JP drain was placed. Result Operative time was forty-five minutes, blood loss was 100 ml. The patient was discharged after 48 hours with no immediate complications. Conclusion The utilization of minimal invasive surgery using the robot to extract multiple pelvicalyceal stones from a horseshoe kidney without reflecting the mesocolon proved to be a feasible and novel way in the management of complex stone disease improving the outcome with minimal morbidity.

  5. Identification of Aphis gossypii Glover (Hemiptera: Aphididae Biotypes from Different Host Plants in North China.

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    Li Wang

    Full Text Available The cotton-melon aphid, Aphis gossypii Glover (Hemiptera: Aphididae, is a polyphagous species with a worldwide distribution and a variety of biotypes. North China is a traditional agricultural area with abundant winter and summer hosts of A. gossypii. While the life cycles of A. gossypii on different plants have been well studied, those of the biotypes of North China are still unclear.Host transfer experiments showed that A. gossypii from North China has two host-specialized biotypes: cotton and cucumber. Based on complete mitochondrial sequences, we identified a molecular marker with five single-nucleotide polymorphisms to distinguish the biotypes. Using this marker, a large-scale study of biotypes on primary winter and summer hosts was conducted. All A. gossypii collected from three primary hosts-hibiscus, pomegranate, and Chinese prickly ash-were cotton biotypes, with more cotton-melon aphids found on hibiscus than the other two species. In May, alate cotton and cucumber biotypes coexisted on cotton and cucumber seedlings, but each preferred its natal host. Both biotypes existed on zucchini, although the cucumber biotype was more numerous. Aphids on muskmelon were all cucumber biotypes, whereas most aphids on kidney bean were cotton biotypes. Aphids on seedlings of potato and cowpea belong to other species. In August, aphids on cotton and cucumber were the respective biotypes, with zucchini still hosting both biotypes as before. Thus, the biotypes had different fitnesses on different host plants.Two host-specialized biotypes (cotton and cucumber are present in North China. Hibiscus, pomegranate, and Chinese prickly ash can serve as winter hosts for the cotton biotype but not the cucumber biotype in North China. The fitnesses of the two host-specialized biotypes differ on various summer hosts. When alate aphids migrate to summer hosts, they cannot accurately land on the corresponding plant.

  6. Conversion of finished leather waste incorporated with plant fibers into value added consumer products - An effort to minimize solid waste in Ethiopia.

    Science.gov (United States)

    Teklay, A; Gebeyehu, G; Getachew, T; Yaynshet, T; Sastry, T P

    2017-07-29

    Presently, the leftovers from leather product industries are discarded as waste in Ethiopia. The objective of the present study was therefore, to prepare composite sheets by incorporating various plant fibers like enset (Ensete ventricosum), hibiscus (Hibiscus cannabinus), jute (Corchorus trilocularis L.), palm (Phoenix dactylifera) and sisal (Agave sisal) in various proportions into the leather waste. Resin binder (RB) and natural rubber latex (NRL) were used as binding agents for the preparation of the composite sheets. The composite sheets prepared were characterized for their physicochemical properties (tensile strength, elongation at break, stitch tear strength, water absorption, water desorption and flexing strength). Composite sheets prepared using RB having 10% hibiscus, 20% palm and 40% sisal fibers showed better mechanical properties than their respective controls. In composite sheets prepared using NRL having 30% jute fiber exhibited better mechanical properties than its control. Most of the plant fibers used in this study played a role in increasing the performance of the sheets. However, as seen from the results, the contribution of these plant fibers on performance of the composite sheets prepared is dependent on the ratio used and the nature of binder. The SEM studies have exhibited the composite nature of the sheets and FTIR studies have shown the functional groups of collagen protein, cellulose and binders. The prepared sheets were used as raw materials for preparation of items like stiff hand bags, ladies' purse, keychain, chappal upper, wallet, wall cover, mouse pad and other interior decorating products. By preparing such value added products, we can reduce solid waste; minimize environmental pollution and thereby securing environmental sustainability. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Inhibition of New Delhi Metallo-β-Lactamase 1 (NDM-1 Producing Escherichia coli IR-6 by Selected Plant Extracts and Their Synergistic Actions with Antibiotics

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    Brinda Chandar

    2017-08-01

    Full Text Available Improper use of antibiotics has led to a great concern in the development of pathogenic microbial resistance. New Delhi metallo-β-lactamase 1 (NDM-1 producing bacteria are resistant to most of the β-lactam antibiotics, and so far, no new compounds have been clinically tested against these bacteria. In this study, ethanol extracts from the leaves of 240 medicinal plant species were screened for antibacterial activity against an NDM-1 Escherichia coli strain. The extracts that showed antibacterial activity were then tested for minimum inhibitory concentrations (MICs and zones of inhibition. The extract from Combretum albidum G. Don, Hibiscus acetosella Welw. ex Hiern, Hibiscus cannabinus L., Hibiscus furcatus Willd., Punica granatum L., and Tamarindus indica L. showed bactericidal activity between 5 and 15 mg/ml and the MIC was between 2.56 and 5.12 mg/ml. All six plant extracts inhibited activity of the NDM-1 enzyme in vitro, and the IC50 value ranged between 0.50 and 1.2 ng/μl. Disruption of bacterial cell wall integrity by the plant extracts was clearly visible with scanning electron microscopy. Increases in membrane permeability caused 79.4–89.7% bacterial cell deaths as investigated by fluorescence-activated cell sorting. All the plant extracts showed synergistic effects when combined with colistin [fractional inhibitory concentration (ΣFIC = 0.125–0.375], meropenem (ΣFIC = 0.09–0.313, and tetracycline (ΣFIC = 0.125–0.313. Thus, the plant extracts can be fractionated for the identification of active compounds, which could be used as new antibacterial compounds for the development of drugs against NDM-1 E. coli in addition to their use in combination therapy.

  8. Mini-percutaneous nephrolithotomy for stones in anomalous-kidneys: a prospective study.

    Science.gov (United States)

    Khadgi, Sanjay; Shrestha, Babu; Ibrahim, Hamdy; Shrestha, Sunil; ElSheemy, Mohammed S; Al-Kandari, Ahmed M

    2016-10-04

    To evaluate safety and efficacy of minipercutaneous nephrolithotomy (Mini-PNL) in management of stones in different types of renal anomalies. Patients with stones ≥2 cm or SWL-resistant stones in anomalous-kidneys treated by Mini-PNL between March 2010 and September 2012 were included prospectively. Mini-PNL was done under regional anesthesia in prone position with fluoroscopic guidance through 18 Fr sheath using semirigid ureteroscope (8.5/11.5 Fr) and pneumatic lithotripter. All patients were followed-up for 2-3 years. Stone-free rate was defined as absence of residual fragments ≥2 mm. Student-T, Mann-Whitney, Chi square (χ (2)), Fisher-exact, one way ANOVA or Kruskal-Wallis test were used for analysis. Mini-PNL was performed for 59 patients (20 horseshoe, 15 malrotated, 7 polycystic, 13 duplex and 4 ectopic pelvic-kidneys). Mean age was 40.18 ± 12.75 (14-78) years. Mean stone burden was 31.72 ± 21.43 (7.85-141.3) mm(2). Two tracts were required in 7 (11.9 %) patients. Tubeless Mini-PNL with double-J insertion was performed in all patients except two. Operative time was 50.17 ± 18.73 (15-105) min. Hemoglobin loss was 0.44 ± 0.30 (0-1.4) g/dL. Complications were reported in 15 (25.4 %) patients. No pleural injury, sepsis, perinephric-collection or renal-pelvis perforation were reported. Stone-free rate was 89.8 % (converted to open-surgery in one patient, second-look PNL in two patients, auxiliary SWL in three patients). Stone-free rate improved to 98.3 % after retreatment and auxiliary SWL. Site of puncture was mostly upper calyceal in horseshoe-kidney (80 %), mid calyceal in polycystic-kidney (85.7 %) and lower calyceal in duplex-kidney (46.2 %). Punctures were also significantly infracostal in horseshoe-kidney (100 %) and supracostal in both duplex (53.8 %) and malrotated-kidneys (66.7 %). Mini-PNL is safe for management of stones in anomalous-kidney with SFR comparable to standard-PNL but with less complications.

  9. Anatomy of the collecting system of lower pole of the kidney in patients with a single renal stone: a comparative study with individuals with normal kidneys

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    Zomorrodi Afshar

    2010-01-01

    Full Text Available At least 5% of women and 12% of men during their lives will experience renal colic, at least once. Many theories have been suggested for the etiology of renal stones and va-riations in the anatomy of the collecting system have been suggested to have a role in stone formation. This study was conducted to examine the role of variation of lower pole collecting system in patients with lower pole kidney stone and compared the same in normal persons (kidney donors. Investigation for the anatomy of the lower pole of the kidney (angle between lower infundibulum and pelvis, length and diameter of the infundibulum and number and pattern distribution of calyces was carried out using intravenous pyelogram (IVP in 100 cases with urinary stone (study cases and 400 persons with normal kidneys (control subjects. The study was a retrospective cross-sectional case control study. Results were analyzed by Mann-Whiteny and independent sample chi square tests. The mean infundibulum-pelvic angle (IPA in control subjects and in patients was 112.5 ± 10.7 and 96.6 ± 28.8, respectively. There was significant correlation between reduced angle and stone formation (P= < 0.001. The mean infundibulum-uretero-pelvic angle (IUPA in control subjects and study cases was 53.5 ± 12.7 and 42.6 ± 13.4, respectively. There was significant correlation between decreased angle and stone formation (P= < 0.001. The mean length of infundibulum of lower pole of kidney (IPIL in controls and study patients was 22.5 ± 4.1 and 27.5 ± 7.7, respectively, which was statistically significant (P< 0.001. The mean number of calyces in lower pole of the kidney (LPCN in controls and study patients was 2.6 ± 0.6 and 3 ± 0.9, respectively, which was statistically significant (P= < 0.002. There was no significant correlation between distribution of calyces and stone formation (P= 0.366. Our study suggests that abnormal renal anatomy was more common in patients with lower pole kidney stone and should

  10. Afferent innervation of the utricular macula in pigeons

    Science.gov (United States)

    Si, Xiaohong; Zakir, Mridha Md; Dickman, J. David

    2003-01-01

    Biotinylated dextran amine (BDA) was used to retrogradely label afferents innervating the utricular macula in adult pigeons. The pigeon utriclar macula consists of a large rectangular-shaped neuroepithelium with a dorsally curved anterior edge and an extended medioposterior tail. The macula could be demarcated into several regions based on cytoarchitectural differences. The striola occupied 30% of the macula and contained a large density of type I hair cells with fewer type II hair cells. Medial and lateral extrastriola zones were located outside the striola and contained only type II hair cells. A six- to eight-cell-wide band of type II hair cells existed near the center of the striola. The reversal line marked by the morphological polarization of hair cells coursed throughout the epithelium, near the peripheral margin, and through the center of the type II band. Calyx afferents innervated type I hair cells with calyceal terminals that contained between 2 and 15 receptor cells. Calyx afferents were located only in the striola region, exclusive of the type II band, had small total fiber innervation areas and low innervation densities. Dimorph afferents innervated both type I and type II hair cells with calyceal and bouton terminals and were primarily located in the striola region. Dimorph afferents had smaller calyceal terminals with few type I hair cells, extended fiber branches with bouton terminals and larger innervation areas. Bouton afferents innervated only type II hair cells in the extrastriola and type II band regions. Bouton afferents innervating the type II band had smaller terminal fields with fewer bouton terminals and smaller innervation areas than fibers located in the extrastriolar zones. Bouton afferents had the most bouton terminals on the longest fibers, the largest innervation areas with the highest innervation densities of all afferents. Among all afferents, smaller terminal innervation fields were observed in the striola and large fields were

  11. A preliminary survey of primitive crops cultivated in the northern Transvaal of South Africa

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    T. H. Arnold

    1983-12-01

    Full Text Available The different tribal economies of South Africa rely extensively on a number of primitive crop taxa which are cultivated as a primary food source. The most important of these include  Sorghum bicolor,  Pennisetum americanum, Citrullus lanatus, Lagenaria siceraria, Vigna unguiculata, Voandzeia subterranea and  Hibiscus esculentus. Morphological variation within these and a number of less important crops is discussed. The frequency with which each crop is grown and preference ratings allotted to them by individual tribal families are compared between the three major ethnic regions of the northern Transvaal. Factors which determine preferences are also discussed and suggestions made relating to germ plasm conservation.

  12. Dicty_cDB: Contig-U15920-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 358938 ) Schizaphis graminum elongation factor 1 alpha gen... 44 10.0 1 ( EU358933 ) Myzus persicae elongati...44 10.0 1 ( EU019868 ) Aphis gossypii from Hibiscus mutabilis elongation... 44 10.0 1 ( EF660855 ) Myzus per...sicae isolate F001 lineage (green) elong... 44 10.0 1 ( EF660854 ) Myzus persicae isolate G006 lineage (gree...n) elong... 44 10.0 1 ( EF660853 ) Myzus persicae isolate USDA tobacco adapted li...his craccivora isolate Nanjing population elong... 44 10.0 1 ( EF419315 ) Myzus persicae isolate Fuzhou popu

  13. Evaluation of the Effect of Different Herbal Tea on Discoloration of Different Kinds of Composite

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    Fatemeh Nezhadnasrollah

    2016-09-01

    Full Text Available Objectives: The aim of this study was to evaluate the effect of six herbal teas on the color stability of two types of nanohybrid and one microhybrid resin composite.  Materials and Methods: 70 disc-shaped specimens, 210 in total (7*2mm, were fabricated from each of the following materials in metal mould : Tetric N ceram, Grandio, Gradia Direct Anterior. Specimens were stored in distilled water at 37°C for 24 hours in an incubator for completion of polymerization. After baseline evaluation (L*, a*, b*CIELAB scale, the specimens were divided into seven subgroups, according to the test and control storage solutions (n=10. Randomly selected specimens from each material were immersed in 20 ml of the test solutions (Borago, Green, Hibiscus, Thyme, Black and Lemon Verbena teas at 37˚c for 24 hours and 48 hours. Solutions were refreshed every 24 hours. All samples were polished using Soflex discs with Medium, Fine, Superfine grit after storage in herbal teas. Specimens color was measured in 24, 48 hours and after polishing. The collected data was statistically analyzed using two-way analysis of variance with repeated measure and Tukey’s HSD at a significance level of 0.05. Results: All samples displayed color changes after immersion in the herbal teas. Hibiscus tea induced the highest level of discoloration after 24 hours immersion in all three composites. Black tea induced highest level of discoloration in (Grandio ΔE=7.44. Hibiscus tea and Thyme tea induced highest level of discoloration in (Tetric N ceram ΔE=11. and (Gradia Direct ΔE=14.11, respectively, after 48 hours immersion. The least discoloration was found with Borage tea in 24 and 48 hours. After re-polishing the color change was reduced. Grandio showed the greatest color reduction in Black tea. Color improvement of Tetric N ceram was better than Gradia Direct. Conclusion: All tested restorative materials showed a color shift after immersion in herbal teas, which Tetric N ceram

  14. Formulación de un medio de cultivo anaerobio para protozoarios ruminales y evaluación in vitro en la capacidad desfaunante del extracto de plantas

    OpenAIRE

    Alejandro Ley de Coss; Mario Antonio Cobos Peralta; David Hernández Sánchez; Enrique Guerra Medina

    2011-01-01

    Se evaluó la efectividad de un medio de cultivo anaerobio enriquecido con el extracto soluble de Avena sativa (Medio de cultivo testigo, MCT) para mantener viables a los protozoarios ciliados del rumen y para evaluar la capacidad desfaunante de los extractos solubles en agua de Argemone mexicana, Baccharis vaccinioides, Hibiscus rosa-sinesis, Montanoa leucanta, Morus alba y Yucca schidigera. La propiedad desfaunante se estimó inoculando 0,5 mL de un concentrado de protozoarios en 9,5 mL de MC...

  15. Inhibition by natural dietary substances of gastrointestinal absorption of starch and sucrose in rats and pigs: 1. Acute studies.

    Science.gov (United States)

    Preuss, Harry G; Echard, Bobby; Bagchi, Debasis; Stohs, Sidney

    2007-08-06

    Rapid gastrointestinal absorption of refined carbohydrates (CHO) is linked to perturbed glucose-insulin metabolism that is, in turn, associated with many chronic health disorders. We assessed the ability of various natural substances, commonly referred to as "CHO blockers," to influence starch and sucrose absorption in vivo in ninety-six rats and two pigs. These natural enzyme inhibitors of amylase/sucrase reportedly lessen breakdown of starches and sucrose in the gastrointestinal tract, limiting their absorption. To estimate absorption, groups of nine SD rats were gavaged with water or water plus rice starch and/or sucrose; and circulating glucose was measured at timed intervals thereafter. For each variation in the protocol a total of at least nine different rats were studied with an equal number of internal controls on three different occasions. The pigs rapidly drank CHO and inhibitors in their drinking water. In rats, glucose elevations above baseline over four hours following rice starch challenge as estimated by area-under-curve (AUC) were 40%, 27%, and 85% of their internal control after ingesting bean extract, hibiscus extract, and l-arabinose respectively in addition to the rice starch. The former two were significantly different from control. L-Arabinose virtually eliminated the rising circulating glucose levels after sucrose challenge, whereas hibiscus and bean extracts were associated with lesser decreases than l-arabinose that were still significantly lower than control. The glucose elevations above baseline over four hours in rats receiving sucrose (AUC) were 51%, 43% and 2% of control for bean extract, hibiscus extract, and L-arabinose, respectively. Evidence for dose-response of bean and hibiscus extracts is reported. Giving the natural substances minus CHO challenge caused no significant changes in circulating glucose concentrations, indicating no major effects on overall metabolism. A formula combining these natural products significantly

  16. Inhibition by Natural Dietary Substances of Gastrointestinal Absorption of Starch and Sucrose in Rats and Pigs: 1. Acute Studies

    Directory of Open Access Journals (Sweden)

    Harry G. Preuss, Bobby Echard, Debasis Bagchi, Sidney Stohs

    2007-01-01

    Full Text Available Rapid gastrointestinal absorption of refined carbohydrates (CHO is linked to perturbed glucose-insulin metabolism that is, in turn, associated with many chronic health disorders. We assessed the ability of various natural substances, commonly referred to as “CHO blockers,” to influence starch and sucrose absorption in vivo in ninety-six rats and two pigs. These natural enzyme inhibitors of amylase/sucrase reportedly lessen breakdown of starches and sucrose in the gastrointestinal tract, limiting their absorption. To estimate absorption, groups of nine SD rats were gavaged with water or water plus rice starch and/or sucrose; and circulating glucose was measured at timed intervals thereafter. For each variation in the protocol a total of at least nine different rats were studied with an equal number of internal controls on three different occasions. The pigs rapidly drank CHO and inhibitors in their drinking water. In rats, glucose elevations above baseline over four hours following rice starch challenge as estimated by area-under-curve (AUC were 40%, 27%, and 85% of their internal control after ingesting bean extract, hibiscus extract, and l-arabinose respectively in addition to the rice starch. The former two were significantly different from control. L-Arabinose virtually eliminated the rising circulating glucose levels after sucrose challenge, whereas hibiscus and bean extracts were associated with lesser decreases than l-arabinose that were still significantly lower than control. The glucose elevations above baseline over four hours in rats receiving sucrose (AUC were 51%, 43% and 2% of control for bean extract, hibiscus extract, and L-arabinose, respectively. Evidence for dose-response of bean and hibiscus extracts is reported. Giving the natural substances minus CHO challenge caused no significant changes in circulating glucose concentrations, indicating no major effects on overall metabolism. A formula combining these natural products

  17. Modificación química de carbones activados con ácidos minerales

    OpenAIRE

    Omenat Morán, Delia

    2016-01-01

    Se han preparado carbón vegetal y carbones activados a partir de médula de kenaf (Hibiscus cannabinus L.). Los carbones activados han sido después químicamente modificados con ácidos minerales y también se ha realizado esta modificación sobre un carbón activado comercial microporoso. Los carbones así preparados han sido caracterizados y ensayados en diversas aplicaciones. Los obtenidos a partir de kenaf se caracterizan por ser macroporosos. El carbón comercial modificado ha actuado como catal...

  18. Antifungal Activity of Volatile Components Extracted from Leaves, Stems and Flowers of Four Plants Growing in Tunisia

    Directory of Open Access Journals (Sweden)

    N. Boughalleb

    2005-12-01

    Full Text Available Volatile components extracted from the leaves, stems and flowers of Lantana camara, Malvaviscus arboreus, Hibiscus rosa-sinensis cv. red flowers and white flowers were tested against the fungi Alternaria solani, Botrytis cinerea, Fusarium solani f. sp. cucurbitae, F. oxysporum f. sp. niveum, Pythium ultimum, Rhizoctonia solani and Verticillium dahliae. The strongest inhibitory effect of the extracts was found with volatile components extracted from the stems and the flowers. Complete inhibition was achieved against V. dahliae. The weakest effect was against P. ultimum. Volatile components extracted from the leaves were not effective.

  19. Environmental-friendly wool fabric finishing by some water plant extracts

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    Šmelcerović Miodrag

    2007-01-01

    Full Text Available In this article, environmental-friendly finishing of wool fabric were processed with several water extract plants, such as hibiscus, St. John's wort, and marigold. The plant extracts have good basis in the commercial dyeing of wool, for garment and carpet industry. At the same time, the environmental-friendly finishing by water extracts plants shows very good fastness of the antimicrobial properties and coloration of wool fabric. From an ecological viewpoint, the substitution of chemical dyes with "natural products" may represent not only a strategy to reduce risk and pollutants but also an opportunity for new markets and new businesses, which can expend involving of ecology in trade policy.

  20. A preliminary survey of primitive crops cultivated in the northern Transvaal of South Africa

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    T. H. Arnold

    1983-12-01

    Full Text Available The different tribal economies of South Africa rely extensively on a number of primitive crop taxa which are cultivated as a primary food source. The most important of these include  Sorghum bicolor,  Pennisetum americanum, Citrullus lanatus, Lagenaria siceraria, Vigna unguiculata, Voandzeia subterranea and  Hibiscus esculentus. Morphological variation within these and a number of less important crops is discussed. The frequency with which each crop is grown and preference ratings allotted to them by individual tribal families are compared between the three major ethnic regions of the northern Transvaal. Factors which determine preferences are also discussed and suggestions made relating to germ plasm conservation.

  1. Genitourinary tuberculosis - report of two cases; Tuberculose genitourinaria - relato de dois casos

    Energy Technology Data Exchange (ETDEWEB)

    Turtelli, Celso Montenegro; Fonseca, Cristiano Rezio; Barbosa, Luciana; Nogueira, Andre Messias; Furtado, Rogerio Abrao; Rehder, Gustavo Frederico Correa; Correa, Adriana Cristina; Tiveron, Gustavo Roberto Carvalho [Faculdade de Medicina do Triangulo Mineiro (FMTM), Uberaba, MG (Brazil)

    2000-06-01

    The incidence of urogenital tuberculosis has decreased in recent years. However, due to insidious character, it remains clinically important. The recognition of the multiple radiological findings is of great importance. In the early stages, the excretory urogram may be normal in spite of positive urine cultures for acid-fast organisms. Subsequently, the calyces may present irregularities. The use of ultrasonography in the evaluation of renal tuberculosis has been sporadically described in the literature. It can determine the site of the obstruction when there is a nonfunctioning kidney. Computed tomography may demonstrate the presence and the site of the obstruction, and evaluate any remaining renal function after injection of contrast medium. Its ability to detect calcification surpasses that of plain films. The renal parenchyma may also be evaluated in the obstructed kidney. (author)

  2. Six new physalins from Physalis alkekengi var. franchetii and their cytotoxicity and antibacterial activity.

    Science.gov (United States)

    Yang, Ye-Kun; Xie, Si-da; Xu, Wan-Xuan; Nian, Yin; Liu, Xiao-Ling; Peng, Xing-Rong; Ding, Zhong-Tao; Qiu, Ming-Hua

    2016-07-01

    Six new physalin steroids, 7β-methoxylisophysalin B (1), 7β-methoxylphysalin C (2), physalin V (3), physalin VI (4), physalin VII (5), isophysalin I (6), together with 20 known physalins (7-26) were isolated from calyces of Physalis alkekengi var. franchetii. Structures of the new compounds were revealed through 1D and 2D NMR and mass spectroscopic methods. Compounds 1-26 were evaluated for cytotoxicity against human HL-60, SMMC-7721, A-549, MCF-7 and SW-480, and the results indicated that compounds 8, 11, and 14 displayed potent cytotoxicities (IC50<5μM) in vitro. Further antibacterial assay indicated that compounds 8, 14, and 19 showed high antibacterial activities against Bacillus subtilis and Escherichia coli.

  3. In vitro studies on the relationship between the anti-inflammatory activity of Physalis peruviana extracts and the phagocytic process.

    Science.gov (United States)

    Martínez, Willington; Ospina, Luis Fernando; Granados, Diana; Delgado, Gabriela

    2010-03-01

    The study of plants used in traditional medicine has drawn the attention of researchers as an alternative in the development of new therapeutics agents, such as the American Solanaceae Physalis peruviana, which has significant anti-inflammatory activity. The Physalis peruviana anti-inflammatory effect of ethanol or ether calyces extracts on the phagocytic process was assessed by using an in vitro phagocytosis model (Leishmania panamensis infection to murine macrophages). The Physalis peruviana extracts do not inhibit microorganism internalization and have no parasiticide effect. Most ET and EP extracts negatively affected the parasite's invasion of macrophages (Infected cells increased.). This observation might result from a down-regulation of the macrophage's microbicide ability associated with a selective reduction of proinflammatory cytokines levels. Physalis peruviana's anti-inflammatory activity described in this model is related to an immunomodulatory effect exerted on macrophages infected, which directly or indirectly "blocks" their ability to secrete soluble proinflammatory mediators.

  4. Extracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae.

    Directory of Open Access Journals (Sweden)

    Jean-Claude Caissard

    Full Text Available Sclareol is a high-value natural product obtained by solid/liquid extraction of clary sage (Salvia sclarea L. inflorescences. Because processes of excretion and accumulation of this labdane diterpene are unknown, the aim of this work was to gain knowledge on its sites of accumulation in planta. Samples were collected in natura or during different steps of the industrial process of extraction (steam distillation and solid/liquid extraction. Samples were then analysed with a combination of complementary analytical techniques (gas chromatography coupled to a mass spectrometer, polarized light microscopy, environmental scanning electron microscopy, two-photon fluorescence microscopy, second harmonic generation microscopy. According to the literature, it is hypothesized that sclareol is localized in oil pockets of secretory trichomes. This study demonstrates that this is not the case and that sclareol accumulates in a crystalline epicuticular form, mostly on calyces.

  5. Extracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae).

    Science.gov (United States)

    Caissard, Jean-Claude; Olivier, Thomas; Delbecque, Claire; Palle, Sabine; Garry, Pierre-Philippe; Audran, Arthur; Valot, Nadine; Moja, Sandrine; Nicolé, Florence; Magnard, Jean-Louis; Legrand, Sylvain; Baudino, Sylvie; Jullien, Frédéric

    2012-01-01

    Sclareol is a high-value natural product obtained by solid/liquid extraction of clary sage (Salvia sclarea L.) inflorescences. Because processes of excretion and accumulation of this labdane diterpene are unknown, the aim of this work was to gain knowledge on its sites of accumulation in planta. Samples were collected in natura or during different steps of the industrial process of extraction (steam distillation and solid/liquid extraction). Samples were then analysed with a combination of complementary analytical techniques (gas chromatography coupled to a mass spectrometer, polarized light microscopy, environmental scanning electron microscopy, two-photon fluorescence microscopy, second harmonic generation microscopy). According to the literature, it is hypothesized that sclareol is localized in oil pockets of secretory trichomes. This study demonstrates that this is not the case and that sclareol accumulates in a crystalline epicuticular form, mostly on calyces.

  6. Renal pelvic stones: choosing shock wave lithotripsy or percutaneous nephrolithotomy

    Directory of Open Access Journals (Sweden)

    Robert Marcovich

    2003-06-01

    Full Text Available Introduction of minimally invasive techniques has revolutionized the surgical management of renal calculi. Extracorporeal shock wave lithotripsy and percutaneous nephrolithotomy are now both well-established procedures. Each modality has advantages and disadvantages, and the application of each should be based on well-defined factors. These variables include stone factors such as number, size, and composition; factors related to the stone's environment, including the stone's location, spatial anatomy of the renal collecting system, presence of hydronephrosis, and other anatomic variables, such as the presence of calyceal diverticula and renal anomalies; and clinical or patient factors like morbid obesity, the presence of a solitary kidney, and renal insufficiency. The morbidity of each procedure in relation to its efficacy should be taken in to account. This article will review current knowledge and suggest an algorithm for the rational management of renal calculi with shock wave lithotripsy and percutaneous nephrolithotomy.

  7. Idiopathic retroperitoneal fibrosis with particular perirenal and intrarenal extension associated with left renal artery stenosis. The atheromatous periaortitis with retroperitoneal fibrosis suggests a pathogenic relationship between atherosclerosis and fibrosis?

    Science.gov (United States)

    Gluhovschi, Gheorghe; Bozdog, Gheorghe; Miclaus, Gratian; Puscasiu, Tudor; Gluhovschi, Cristina; Bob, Flaviu; Velciov, Silvia; Petrica, Ligia; Trandafirescu, Virginia; Gadalean, Florica

    2011-02-01

    We present a case of idiopathic retroperitoneal fibrosis (RPF) in a female patient of 45 years, obese (BMI = 39 kg/sqm), hypertensive since 2005, with diabetes mellitus treated with diet and diabetes insipidus in whom, during a routine control, the following has been found: serum creatinine 1.74 mg/dl, and an inflammatory syndrome associated with fever. Spiral-CT (Multi-slice-Sensation 64) scan shows retroperitoneal fibrosis in relation with periaortitis that affects the thoracic and abdominal aorta. RPF is extending perirenally and at the level of the renal hilum with subsequent calyceal dilations (hydrocalycosis) associated with left renal artery stenosis. The particularity of the case is represented by the perirenal and intrarenal evolution of fibrosis with left renal artery stenosis with moderate impairment of renal function reversible under treatment with Tamoxifen. This case, with chronic periaortitis subsequent to an extended aortic atherosclerosis with retroperitoneal fibrosis can be representative for the pathogenic relationship between atherosclerosis and fibrosis.

  8. Sound-sensitive neurons innervate the ventro-lateral protocerebrum of the heliothine moth brain

    DEFF Research Database (Denmark)

    Pfuhl, Gerit; Zhao, Xin Cheng; Ian, Elena

    2014-01-01

    -sensitive neurons in the moth brain. During intracellular recordings from the lateral protocerebrum in the brain of three noctuid moth species, Heliothis virescens, Helicoverpa armigera and Helicoverpa assulta, we found an assembly of neurons responding to transient sound pulses of broad bandwidth. The majority...... of the auditory neurons ascended from the ventral cord and ramified densely within the anterior region of the ventro-lateral protocerebrum. The physiological and morphological characteristics of these auditory neurons were similar. We detected one additional sound-sensitive neuron, a brain interneuron with its...... soma positioned near the calyces of mushroom bodies and with numerous neuronal processes in the ventro-lateral protocerebrum. Mass-staining of ventral-cord neurons supported the assumption that the ventro-lateral region of the moth brain was the main target for the auditory projections ascending from...

  9. A New Non-enzymatic Method to Isolate Immature Embryos of Capsella bursa-pastoris (L.) Medic.%一种新的非酶法分离荠幼胚的方法

    Institute of Scientific and Technical Information of China (English)

    洪亚平; 张亚冰; 吴国锋; 赵文娟; 阎婵婵

    2009-01-01

    [Objective] The aim of this study was to manually isolate the microstructures such as ovule and embryo of Capsella bursa-pastoris (L.) Medic. by the non-enzymatic method. [Method] With the gum block and dissecting microscope, its floral characteristics, ovule and embryo were manually isolated and observed by the non-enzymatic method. [Result] C. bursa-pastoris had four calyces, four petals, tetradynamous stamen and silicle, while its embryogenesis contained globular embryo, heart-shaped embryo and torpedo-shaped embryo. [Conclusion] The microstructures such as ovule and embryo of plants become easier to be isolated manually with the viscosity of gum block, which has small damage on embryo or disadvantages of keeping original shapes, and also can used in multi fields studies. This test can also be accomplished in labs with poor experiment facilities.

  10. The time of infection of apples by Botrytis cinerea Pers.

    Directory of Open Access Journals (Sweden)

    Hanna Bryk

    2013-12-01

    Full Text Available The time of infection of apple fruits by Botrytis cinerea Pers. was studied. Artificial inoculations with conidial suspensions of B. cinerea were done at different stages of fruit developmment (flowers, sets, fruits. In autumn the apples were harvested and stored at a temperature of 2°C for 4 months after which rotting caused by B. cinerea was evaluated. B. cinerea presence in the calyx of apples was checked throughout the growing season. This was done by plating flowers, apple and set calyces on PDA medium. Latent infection of apples by B. cinerea was found. The infection took place in the orchard and the pathogen survived latently in the calyx; disease symptoms appeared in storage. Infection is possible during the whole vegetative season, but the most important time is flowering and just before or just after harvest of apples.

  11. Extracellular Localization of the Diterpene Sclareol in Clary Sage (Salvia sclarea L., Lamiaceae)

    Science.gov (United States)

    Caissard, Jean-Claude; Olivier, Thomas; Delbecque, Claire; Palle, Sabine; Garry, Pierre-Philippe; Audran, Arthur; Valot, Nadine; Moja, Sandrine; Nicolé, Florence; Magnard, Jean-Louis; Legrand, Sylvain; Baudino, Sylvie; Jullien, Frédéric

    2012-01-01

    Sclareol is a high-value natural product obtained by solid/liquid extraction of clary sage (Salvia sclarea L.) inflorescences. Because processes of excretion and accumulation of this labdane diterpene are unknown, the aim of this work was to gain knowledge on its sites of accumulation in planta. Samples were collected in natura or during different steps of the industrial process of extraction (steam distillation and solid/liquid extraction). Samples were then analysed with a combination of complementary analytical techniques (gas chromatography coupled to a mass spectrometer, polarized light microscopy, environmental scanning electron microscopy, two-photon fluorescence microscopy, second harmonic generation microscopy). According to the literature, it is hypothesized that sclareol is localized in oil pockets of secretory trichomes. This study demonstrates that this is not the case and that sclareol accumulates in a crystalline epicuticular form, mostly on calyces. PMID:23133579

  12. Unusual case of nephrocutaneous fistula – Our experience

    Directory of Open Access Journals (Sweden)

    Rajeev Thekumpadam Puthenveetil

    2016-01-01

    Full Text Available A rare case of nephrococutaneous fistula due to spontaneous expulsion of renal calculi is described. A 45-year-old man presented with urinary leakage from an ulcer over the left lumbar region for the last 3 months after a history of spontaneous expulsion of stones from this area. Ultrasonography abdomen revealed a small contracted kidney with multiple calculi in the kidney and renal pelvis, sinus tract from the lower pole of the left kidney with a ruptured calyceal calculus in the sinus tract. CT urography revealed a non excreting left kidney with multiple renal calculi, with hyperdense collection in the renal parenchyma extending to the subcutaneous tissue and left lung suggesting a xenthogranulomatous pyelonephritis (XGP. We performed a left-sided simple nephrectomy with excision of the fistulous tract. Histopathological examination revealed XGP. There have been a few case reports of XGP forming nephrocutaneous fistula in the back.

  13. Acute management of stones

    DEFF Research Database (Denmark)

    Jung, Helene; Osther, Palle J S

    2015-01-01

    INTRODUCTION: Stone management is often conservative due to a high spontaneous stone passage rate or non-symptomatic calyceal stones that do not necessarily require active treatment. However, stone disease may cause symptoms and complications requiring urgent intervention. MATERIAL AND METHODS......: In this review, we update latest research and current recommendations regarding acute management of stones, with particular focus on imaging, pain management, active stone interventions, medical expulsive therapy, and urolithiasis in pregnancy and childhood. RESULTS: Acute stone management should be planned...... with careful consideration of stone size and location, symptoms, patient comorbidity and radiation dose. CONCLUSION: In case of infective hydronephrosis, compromised renal function or persistent pain despite adequate analgesic treatment acute intervention is indicated....

  14. Renal papillary necrosis in patients with sickle cell disease: How to recognize this 'forgotten' diagnosis.

    Science.gov (United States)

    Henderickx, Michaël M E L; Brits, Tim; De Baets, Karen; Seghers, Mattias; Maes, Philip; Trouet, Dominique; De Wachter, Stefan; De Win, Gunter

    2017-06-01

    Renal papillary necrosis is not commonly seen in daily practice, but can have severe consequences when it is not diagnosed in time. It is known to be associated with sickle cell hemoglobinopathies; however a wide range of etiologies are possible, and it is therefore not the first diagnosis clinicians consider in patients with sickle cell disease who present with hematuria. A literature search was performed to summarize the current knowledge about renal papillary necrosis associated with sickle cell disease. These findings are illustrated with a case of a 9-year old girl with sickle cell disease who was referred with painless gross hematuria. Typical radiologic signs for renal papillary necrosis are necrotic cavities that fill with contrast, small collections of contrast peripheral to the calyces in the papillary region (ball-on-tee sign), calcification of the papillary defect, filling defects, hydronephrosis, blunted papillary tip, clefts in the renal medulla filled with contrast, hyperattenuated medullary calcifications, non-enhanced lesions surrounded by rings of excreted contrast, and clubbed calyces. This study focuses on the pathophysiology of renal papillary necrosis associated with sickle cell disease, the possible symptoms, as well as the diagnostic steps, with a special interest in particular presentation on old (retrograde pyelography) and new (computed tomography) gold standard in radiologic imaging, and the management for this pathology. This study aims to remind clinicians of this "forgotten" diagnosis and what signs to look for in pediatric patients with sickle cell disease who present with hematuria. In pediatric cases radiation protection is important, therefore knowing what radiologic signs can be found on retrograde pyelography can lead to early identification of this pathology without having to proceed to computed tomography. Copyright © 2017 Journal of Pediatric Urology Company. Published by Elsevier Ltd. All rights reserved.

  15. Daily thermal fluctuations experienced by pupae via rhythmic nursing behavior increase numbers of mushroom body microglomeruli in the adult ant brain

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    Agustina eFalibene

    2016-04-01

    Full Text Available Social insects control brood development by using different thermoregulatory strategies. Camponotus mus ants expose their brood to daily temperature fluctuations by translocating them inside the nest following a circadian rhythm of thermal preferences. At the middle of the photophase brood is moved to locations at 30.8°C; 8 h later, during the night, the brood is transferred back to locations at 27.5°C. We investigated whether daily thermal fluctuations experienced by developing pupae affect the neuroarchitecture in the adult brain, in particular in sensory input regions of the mushroom bodies (MB calyces. The complexity of synaptic microcircuits was estimated by quantifying MB-calyx volumes together with densities of presynaptic boutons of microglomeruli (MG in the olfactory lip and visual collar regions. We compared young adult workers that were reared either under controlled daily thermal fluctuations of different amplitudes, or at different constant temperatures. Thermal regimes significantly affected the large (non-dense olfactory lip region of the adult MB calyx, while changes in the dense lip and the visual collar were less evident. Thermal fluctuations mimicking the amplitudes of natural temperature fluctuations via circadian rhythmic translocation of pupae by nurses (amplitude 3.3°C lead to higher numbers of MG in the MB calyces compared to those in pupae reared at smaller or larger thermal amplitudes (0.0, 1.5, 9.6°C, or at constant temperatures (25.4, 35.0°C. We conclude that rhythmic control of brood temperature by nursing ants optimizes brain development by increasing MG densities and numbers in specific brain areas. Resulting differences in synaptic microcircuits are expected to affect sensory processing and learning abilities in adult ants, and may also promote interindividual behavioral variability within colonies.

  16. Daily Thermal Fluctuations Experienced by Pupae via Rhythmic Nursing Behavior Increase Numbers of Mushroom Body Microglomeruli in the Adult Ant Brain.

    Science.gov (United States)

    Falibene, Agustina; Roces, Flavio; Rössler, Wolfgang; Groh, Claudia

    2016-01-01

    Social insects control brood development by using different thermoregulatory strategies. Camponotus mus ants expose their brood to daily temperature fluctuations by translocating them inside the nest following a circadian rhythm of thermal preferences. At the middle of the photophase brood is moved to locations at 30.8°C; 8 h later, during the night, the brood is transferred back to locations at 27.5°C. We investigated whether daily thermal fluctuations experienced by developing pupae affect the neuroarchitecture in the adult brain, in particular in sensory input regions of the mushroom bodies (MB calyces). The complexity of synaptic microcircuits was estimated by quantifying MB-calyx volumes together with densities of presynaptic boutons of microglomeruli (MG) in the olfactory lip and visual collar regions. We compared young adult workers that were reared either under controlled daily thermal fluctuations of different amplitudes, or at different constant temperatures. Thermal regimes significantly affected the large (non-dense) olfactory lip region of the adult MB calyx, while changes in the dense lip and the visual collar were less evident. Thermal fluctuations mimicking the amplitudes of natural temperature fluctuations via circadian rhythmic translocation of pupae by nurses (amplitude 3.3°C) lead to higher numbers of MG in the MB calyces compared to those in pupae reared at smaller or larger thermal amplitudes (0.0, 1.5, 9.6°C), or at constant temperatures (25.4, 35.0°C). We conclude that rhythmic control of brood temperature by nursing ants optimizes brain development by increasing MG densities and numbers in specific brain areas. Resulting differences in synaptic microcircuits are expected to affect sensory processing and learning abilities in adult ants, and may also promote interindividual behavioral variability within colonies.

  17. Higher brain centers for social tasks in worker ants, Camponotus japonicus.

    Science.gov (United States)

    Nishikawa, Michiko; Watanabe, Hidehiro; Yokohari, Fumio

    2012-05-01

    Ants, eusocial insects, have highly elaborate chemical communication systems using a wide variety of pheromones. In the carpenter ant, Camponotus japonicus, workers and queens have the female-specific basiconic sensilla on antennae. The antennal lobe, the primary processing center, in female carpenter ants contains about 480 glomeruli, which are divided into seven groups (T1–T7 glomeruli) based on sensory afferent tracts. The axons of sensory neurons in basiconic sensilla are thought to project to female-specific T6 glomeruli. Therefore, these sensilla and glomeruli are thought to relate to female-specific social tasks in the ants. By using dye filling into local neurons (LNs) and projection neurons (PNs) in the antennal lobe, we neuroanatomically revealed the existence of an isolated processing system for signals probably relating to social tasks in the worker ant. In the antennal lobe, two categories of glomeruli, T6 glomeruli and non-T6 glomeruli, are clearly segregated by LNs. Furthermore, axon terminals of uniglomerular PNs from the respective categories of glomeruli (T6 uni-PNs and non-T6 uni-PNs) are also segregated in the secondary olfactory centers, the calyces of the mushroom body and the lateral horn: T6 uni-PNs terminate in the outer layers of the basal ring and lip of mushroom body calyces and in the posterior region of the lateral horn, whereas non-T6 uni-PNs terminate in the middle and inner layers of the basal ring and lip and in the anterior region of the lateral horn. These findings suggest that information probably relating to social tasks might be isolated from other olfactory information and processed in a separate subsystem.

  18. Simultaneous laparoscopic management of ureteropelvic junction obstruction and renal lithiasis: the combined experience of two academic centers and review of the literature

    Directory of Open Access Journals (Sweden)

    Stravodimos KG

    2014-05-01

    Full Text Available Konstantinos G Stravodimos,1 Stilianos Giannakopoulos,2 Stavros I Tyritzis,1 Aristeides Alevizopoulos,1 Stefanos Papadoukakis,1 Stavros Touloupidis,2 Constantinos A Constantinides11Department of Urology, Athens University Medical School, Laiko Hospital, Athens, 2Department of Urology, Democritus University of Thrace, Alexandroupolis, GreeceIntroduction: Approximately one out of five patients with ureteropelvic junction obstruction (UPJO present lithiasis in the same setting. We present our outcomes of simultaneous laparoscopic management of UPJO and pelvic or calyceal lithiasis and review the current literature.Methods: Thirteen patients, with a mean age of 42.8±13.3 years were diagnosed with UPJO and pelvic or calyceal lithiasis. All patients were subjected to laparoscopic dismembered Hynes–Anderson pyeloplasty along with removal of single or multiple stones, using a combination of laparoscopic graspers, irrigation, and flexible nephroscopy with nitinol baskets.Results: The mean operative time was 218.8±66 minutes. In two cases, transposition of the ureter due to crossing vessels was performed. The mean diameter of the largest stone was 0.87±0.25 cm and the mean number of stones retrieved was 8.2 (1–32. Eleven out of 13 patients (84.6% were rendered stone-free. Complications included prolonged urine output from the drain in one case (Clavien grade I and urinoma formation requiring drainage in another case (Clavien grade IIIa. The mean postoperative follow-up was 30.2 (7–51 months. No patient has experienced stone or UPJO recurrence.Conclusion: Laparoscopy for the management of UPJO along with renal stone removal seems a very appealing treatment, with all the advantages of minimally invasive surgery. Concomitant renal stones do not affect the outcome of laparoscopic pyeloplasty, at least in the midterm. According to our results and the latest literature data, we advocate laparoscopic management as the treatment of choice for these cases

  19. Studies on the Host Biotypes and Its Cause of Cotton Aphid in Nanjing, China

    Institute of Scientific and Technical Information of China (English)

    LIU Xiang-dong; ZHAI Bao-ping; ZHANG Xiao-xi

    2002-01-01

    The fitness of cotton aphid, Aphis gossypii Glover, on different host plants was studied. The results showed that the cotton aphid population in Nanjing formed host preference bio-types on cucumber and cotton. The aphids from cotton and cucumber could not inter-transplant successfully between the two host plants. The apterous and alate offspring of stem mother from hibiscus could not survive, reproduce and form population normally when they were transplanted to cucumber and water melon, but they could form normally when transplanted to cotton and muskmelon. There was no significant difference between the stylet length of cotton aphids from cucumber and cotton, so the stylet length was not the cause of the host preference. When the aphids originated on hibiscus were transplanted to cotton, water melon and muskmelon, the stylet could reach host phloem to absorb host plant's nutrients, but it couldn't when transplanted to cucumber. Cotton aphid can feed on muskmelon without secretion of water-soluble saliva. It could be concluded that the two host biotypes of cotton aphid came from different winter host plants.

  20. EFFETS DES HUILES ESSENTIELLES SUR LES QUALITES HYGIENIQUES ET ORGANOLEPTIQUES DE LA BOISSON "BISSAP"

    Directory of Open Access Journals (Sweden)

    S.A. ADISSO

    2011-01-01

    Full Text Available This study was conducted in a conservation perspective. Thus, we have submitted 6 samples of "hibiscus" beverage taken from different Market in Cotonou Benin to microbiological and sensory analysis. The microbiological parameters studied (total mesophilic flora (total, total and thermotolerant coliforms (E. coli, staphylococci (Staphylococcus aureus, Salmonella, yeasts and moulds have given widely varying results for beverages analyzed immediately after purchase. In order to evaluate the effect of essential oils from Ocimum basilicum L. (Lamiaceae and Melaleuca leucadendron L. (Myrtaceae on mesophilic and fungal flora, a low dose of oils is added to a sample of the drink containing the sought germs and has stored at 26 ± 1°C or 4 ± 1°C. This study showed that the samples preserved with essential oils have retained their original color with natural flavors for eight days as opposed to controls not treated with essential oils, and are fermented rapidly. The degree of inhibition of mesophilic and fungal flora in different mixtures ranged from 22.87 to 99.59%. Therefore the oils have had a microbiostatic effect on germs initially contained in the drink. However,bactericidal and fungicidal effects of low doses of essential oils have been observed in vitro on strains isolated from the drinking. These results provide a basis for the conservation of Hibiscus "drink.