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Sample records for hg stable isotope

  1. Tracing anthropogenic Hg and Pb input using stable Hg and Pb isotope ratios in sediments of the central Portuguese Margin

    NARCIS (Netherlands)

    Mil-Holmens, M.; Blum, J.; Canário, J.; Caetano, M.; Costa, A.M.; Lebreiro, S.M.; Trancoso, M.; Richter, T.O.; de Stigter, H.; Johnson, M.; Branco, V.; Cesário, R.; Mouro, F.; Mateus, M.; Boer, W.; Melo, Z.

    2013-01-01

    Three short marine sediment cores from the Cascais Submarine Canyon (CSC; cores 252-32 and 252-35) and the Estremadura Spur (core 252-16) on the central Portuguese Margin were analysed for Hg, Pb, Al, and Mn concentrations, and both Pb and Hg stable isotope compositions, in order to reconstruct tren

  2. Microbial Oxidation of Hg(0) - Its Effect on Hg Stable Isotope Fractionation and Methylmercury Production

    Energy Technology Data Exchange (ETDEWEB)

    Yee, Nathan [Rutgers Univ., New Brunswick, NJ (United States); Barkay, Tamar [Rutgers Univ., New Brunswick, NJ (United States); Reinfelder, John [Rutgers Univ., New Brunswick, NJ (United States)

    2016-06-28

    relationship between Hg concentrations and rates of denitrification in enrichment cultures. In part III of our project, we examined in more detail the effects of microbial interactions on Hg transformations. We discovered that both sulfate reducing and iron reducing bacteria coexist in freshwater sediments and both microbial groups contribute to mercury methylation. We showed that mercury methylation by sulfate reducing and iron reducing bacteria are temporally and spatially separated processes. We also discovered that methanogens can methylate mercury. We showed that Methanospirillum hungatei JF-1 methylated Hg at comparable rates, but with higher yields, than those observed for sulfate-reducing bacteria and iron-reducing bacteria. Finally, we demonstrated that syntrophic interactions between different microbial groups increase mercury methylation rates. We showed that Hg methylation rates are stimulated via inter-species hydrogen and acetate transfer (i) from sulfate-reducing bacteria to methanogens and (ii) from fermenters to the sulfate-reducing bacteria. In part IV of the project, we studied Hg bioavailability and Hg isotope fractionation. We demonstrated that thiol-bound Hg is bioavailable to mercury resistant bacteria. We found that uptake of Hg from Hg-glutathione and Hg-cysteine complexes does not require functioning glutathione and cystine/cysteine transport systems. We demonstrated that a wide range of methylmercury complexes (e.g. MeHgOH, MeHg-cysteine, and MeHg-glutathione) are bioavailable to mercury resistant bacteria. The rate of MeHg demethylation varies more between different species of mercury resistant bacteria than among MeHg complexes. We showed that microbial demethylation of MeHg depends more on the species of microorganism than on the types and relative concentrations of thiols or other MeHg ligands present. Finally, we demonstrated that Hg methylation by Geobacter sulfurreducens PCA and Desulfovibrio desulfuricans ND132 imparts mass

  3. Microbial Oxidation of Hg(0) - Its Effect on Hg Stable Isotope Fractionation and Methylmercury Production

    Energy Technology Data Exchange (ETDEWEB)

    Yee, Nathan [Rutgers Univ., New Brunswick, NJ (United States); Barkay, Tamar [Rutgers Univ., New Brunswick, NJ (United States); Reinfelder, John [Rutgers Univ., New Brunswick, NJ (United States)

    2016-06-28

    relationship between Hg concentrations and rates of denitrification in enrichment cultures. In part III of our project, we examined in more detail the effects of microbial interactions on Hg transformations. We discovered that both sulfate reducing and iron reducing bacteria coexist in freshwater sediments and both microbial groups contribute to mercury methylation. We showed that mercury methylation by sulfate reducing and iron reducing bacteria are temporally and spatially separated processes. We also discovered that methanogens can methylate mercury. We showed that Methanospirillum hungatei JF-1 methylated Hg at comparable rates, but with higher yields, than those observed for sulfate-reducing bacteria and iron-reducing bacteria. Finally, we demonstrated that syntrophic interactions between different microbial groups increase mercury methylation rates. We showed that Hg methylation rates are stimulated via inter-species hydrogen and acetate transfer (i) from sulfate-reducing bacteria to methanogens and (ii) from fermenters to the sulfate-reducing bacteria. In part IV of the project, we studied Hg bioavailability and Hg isotope fractionation. We demonstrated that thiol-bound Hg is bioavailable to mercury resistant bacteria. We found that uptake of Hg from Hg-glutathione and Hg-cysteine complexes does not require functioning glutathione and cystine/cysteine transport systems. We demonstrated that a wide range of methylmercury complexes (e.g. MeHgOH, MeHg-cysteine, and MeHg-glutathione) are bioavailable to mercury resistant bacteria. The rate of MeHg demethylation varies more between different species of mercury resistant bacteria than among MeHg complexes. We showed that microbial demethylation of MeHg depends more on the species of microorganism than on the types and relative concentrations of thiols or other MeHg ligands present. Finally, we demonstrated that Hg methylation by Geobacter sulfurreducens PCA and Desulfovibrio desulfuricans ND132 imparts mass

  4. Sources and cycling of mercury in the paleo Arctic Ocean from Hg stable isotope variations in Eocene and Quaternary sediments

    Science.gov (United States)

    Gleason, J. D.; Blum, J. D.; Moore, T. C.; Polyak, L.; Jakobsson, M.; Meyers, P. A.; Biswas, A.

    2017-01-01

    Mercury stable isotopic compositions were determined for marine sediments from eight locations in the Arctic Ocean Basin. Mass dependent fractionation (MDF) and mass independent fractionation (MIF) of Hg stable isotopes were recorded across a variety of depositional environments, water depths, and stratigraphic ages. δ202Hg (MDF) ranges from -2.34‰ to -0.78‰; Δ199Hg (MIF) from -0.18‰ to +0.12‰; and Δ201Hg (MIF) from -0.29‰ to +0.05‰ for the complete data set (n = 33). Holocene sediments from the Chukchi Sea and Morris Jesup Rise record the most negative Δ199Hg values, while Pleistocene sediments from the Central Arctic Ocean record the most positive Δ199Hg values. The most negative δ202Hg values are recorded in Pleistocene sediments. Eocene sediments (Lomonosov Ridge) show some overlap in their Hg isotopic compositions with Quaternary sediments, with a sample of the Arctic Ocean PETM (56 Ma) most closely matching the average Hg isotopic composition of Holocene Arctic marine sediments. Collectively, these data support a terrestrially-dominated Hg source input for Arctic Ocean sediment through time, although other sources, as well as influences of sea ice, atmospheric mercury depletion events (AMDEs), and anthropogenic Hg (in core top samples) on Hg isotopic signatures must also be considered.

  5. Variations in stable isotope fractionation of Hg in food webs of Arctic lakes.

    Science.gov (United States)

    Gantner, Nikolaus; Hintelmann, Holger; Zheng, Wang; Muir, Derek C

    2009-12-15

    Biotic and abiotic fractionation of mercury (Hg) isotopes has recently been shown to occur in aquatic environments. We determined isotope ratios (IRs) of Hg in food webs (zooplankton, chironomids, Arctic char) and sediments of 10 Arctic lakes from four regions and investigated the extent of Hg isotope fractionation. Hg IRs were analyzed by multicollector inductively coupled plasma mass spectrometry (MC-ICP/MS). Hg mass independent fractionation (MIF; Delta(199)Hg) and mass dependent fractionation (MDF; delta(202)Hg) were calculated and compared among samples. IRs of Hg in sediment were characterized mainly by MDF and low MIF (Delta(199)Hg -0.37 to 0.74 per thousand). However, all biota showed evidence of MIF, most pronounced in zooplankton (Delta(199)Hg up to 3.40 per thousand) and char (Delta(199)Hg up to 4.87 per thousand). Zooplankton takes up highly fractionated MeHg directly from the water column, while benthic organisms are exposed to sedimentary Hg, which contains less fractionated Hg. As evidenced by delta(13)C measurements, benthic chironomids make up a large proportion of char diet, explaining in part why MIF(char) meteor impact crater lake (Pingualuk) reflects a "pure" atmospheric Hg signature, which is modified only by aqueous in-lake processes. All other lakes are also affected by terrestrial Hg inputs and sediment processes.

  6. Food preferences and Hg distribution in Chelonia mydas assessed by stable isotopes.

    Science.gov (United States)

    Bezerra, M F; Lacerda, L D; Rezende, C E; Franco, M A L; Almeida, M G; Macêdo, G R; Pires, T T; Rostán, G; Lopez, G G

    2015-11-01

    Mercury (Hg) is a highly toxic pollutant that poses in risk several marine animals, including green turtles (Chelonia mydas). Green turtles are globally endangered sea turtle species that occurs in Brazilian coastal waters as a number of life stage classes (i.e., foraging juveniles and nesting adults). We assessed total Hg concentrations and isotopic signatures ((13)C and (15)N) in muscle, kidney, liver and scute of juvenile green turtles and their food items from two foraging grounds with different urban and industrial development. We found similar food preferences in specimens from both areas but variable Hg levels in tissues reflecting the influence of local Hg backgrounds in food items. Some juvenile green turtles from the highly industrialized foraging ground presented liver Hg levels among the highest ever reported for this species. Our results suggest that juvenile foraging green turtles are exposed to Hg burdens from locally anthropogenic activities in coastal areas.

  7. Determination of MeHg sources to fish in the St. Louis River, MN, USA, using Hg stable isotopes

    Science.gov (United States)

    Mercury contamination in the Great Lakes region has become a prevalent concern due to elevated methylmercury (MeHg) levels in fish. While atmospheric deposition of Hg is ubiquitous, releases from legacy point-sources give rise to numerous Areas of Concern (AOCs) across the Great ...

  8. Kinetics of Hg(II) exchange between organic ligands, goethite, and natural organic matter studied with an enriched stable isotope approach.

    Science.gov (United States)

    Jiskra, Martin; Saile, Damian; Wiederhold, Jan G; Bourdon, Bernard; Björn, Erik; Kretzschmar, Ruben

    2014-11-18

    The mobility and bioavailability of toxic Hg(II) in the environment strongly depends on its interactions with natural organic matter (NOM) and mineral surfaces. Using an enriched stable isotope approach, we investigated the exchange of Hg(II) between dissolved species (inorganically complexed or cysteine-, EDTA-, or NOM-bound) and solid-bound Hg(II) (carboxyl-/thiol-resin or goethite) over 30 days under constant conditions (pH, Hg and ligand concentrations). The Hg(II)-exchange was initially fast, followed by a slower phase, and depended on the properties of the dissolved ligands and sorbents. The results were described by a kinetic model allowing the simultaneous determination of adsorption and desorption rate coefficients. The time scales required to reach equilibrium with the carboxyl-resin varied greatly from 1.2 days for Hg(OH)2 to 16 days for Hg(II)-cysteine complexes and approximately 250 days for EDTA-bound Hg(II). Other experiments could not be described by an equilibrium model, suggesting that a significant fraction of total-bound Hg was present in a non-exchangeable form (thiol-resin and NOM: 53-58%; goethite: 22-29%). Based on the slow and incomplete exchange of Hg(II) described in this study, we suggest that kinetic effects must be considered to a greater extent in the assessment of the fate of Hg in the environment and the design of experimental studies, for example, for stability constant determination or metal isotope fractionation during sorption.

  9. A new approach to understand methylmercury (CH3Hg) sources and transformation pathways: Compound-specific carbon stable isotope analysis by GC-C-IRMS

    Science.gov (United States)

    Baya, P. A.; Point, D.; Amouroux, D. P.; Lebreton, B.; Guillou, G.

    2015-12-01

    Methylmercury (CH3Hg) is a potent neurotoxin which is readily assimilated by organisms and bio-accumulates in aquatic food webs. In humans, consumption of CH3Hg contaminated marine fish is the major route of mercury exposure. However, our understanding of CH3Hg transformation pathways is still incomplete. To close this knowledge gap, we propose to explore the stable carbon isotopic composition (δ13C) of the methyl group of CH3Hg for a better understanding of its sources and transformation mechanisms. The method developed for the determination of the δ13C value of CH3Hg in biological samples involves (i) CH3Hg selective extraction, (ii) derivatization, and (iii) separation by gas chromatography (GC) prior to analysis by combustion isotope ratio mass spectrometry (C-IRMS). We present the figures of merit of this novel method and the first δ13C signatures for certified materials (ERM-CE464, BCR414) and biological samples at different marine trophic levels (i.e., tuna fish, zooplankton). The implications of this new approach to trace the pathways associated with Hg methylation and the mechanisms involved will be discussed.

  10. Stable isotope studies

    Energy Technology Data Exchange (ETDEWEB)

    Ishida, T.

    1992-01-01

    The research has been in four general areas: (1) correlation of isotope effects with molecular forces and molecular structures, (2) correlation of zero-point energy and its isotope effects with molecular structure and molecular forces, (3) vapor pressure isotope effects, and (4) fractionation of stable isotopes. 73 refs, 38 figs, 29 tabs.

  11. Calcium stable isotope geochemistry

    Energy Technology Data Exchange (ETDEWEB)

    Gausonne, Nikolaus [Muenster Univ. (Germany). Inst. fuer Mineralogie; Schmitt, Anne-Desiree [Strasbourg Univ. (France). LHyGeS/EOST; Heuser, Alexander [Bonn Univ. (Germany). Steinmann-Inst. fuer Geologie, Mineralogie und Palaeontologie; Wombacher, Frank [Koeln Univ. (Germany). Inst. fuer Geologie und Mineralogie; Dietzel, Martin [Technische Univ. Graz (Austria). Inst. fuer Angewandte Geowissenschaften; Tipper, Edward [Cambridge Univ. (United Kingdom). Dept. of Earth Sciences; Schiller, Martin [Copenhagen Univ. (Denmark). Natural History Museum of Denmark

    2016-08-01

    This book provides an overview of the fundamentals and reference values for Ca stable isotope research, as well as current analytical methodologies including detailed instructions for sample preparation and isotope analysis. As such, it introduces readers to the different fields of application, including low-temperature mineral precipitation and biomineralisation, Earth surface processes and global cycling, high-temperature processes and cosmochemistry, and lastly human studies and biomedical applications. The current state of the art in these major areas is discussed, and open questions and possible future directions are identified. In terms of its depth and coverage, the current work extends and complements the previous reviews of Ca stable isotope geochemistry, addressing the needs of graduate students and advanced researchers who want to familiarize themselves with Ca stable isotope research.

  12. Mass Independent Fractionation of Hg Isotopes Preserved in the Precambrian

    Science.gov (United States)

    Thibodeau, A. M.; Bergquist, B. A.; Kah, L. C.; Ono, S.; Ghosh, S.; Hazen, R. M.

    2013-12-01

    Mercury (Hg) is a photochemically active, redox-sensitive, chalcophilic metal with complex biogeochemistry that displays a wide range of mass-dependent (MDF) and mass-independent (MIF) stable isotopic fractionation. In the past decade, Hg isotopes have emerged as important tracers of both the sources and cycling of Hg in the modern environment. However, their utility as environmental proxies in ancient rocks remains largely unexplored. The potential of Hg isotopes to inform Precambrian environments derives from the observation that Hg isotopes with odd atomic mass numbers (199Hg and 201Hg) undergo large MIF by the magnetic isotope effect (MIE) and smaller MIF through the nuclear volume effect (NVE). Small MIF produced via NVE has been observed for numerous transformations and is characterized by MIF ratios (Δ199Hg/Δ201Hg) of about 1.6. Large Hg-MIF driven by MIE has been observed during photochemical transformations and is characterized by Δ199Hg/Δ201Hg ratios between 1 and 1.3. This MIF signal is sensitive to a range of environmental conditions, including the amount and type of solar radiation, the presence and type of complexing organic ligands, and the Hg/dissolved organic carbon (DOC) ratio. Thus, it is hoped that Hg-MIF signals may indirectly record changes in atmospheric composition or seawater chemistry if preserved in marine sedimentary records. Previous work has clearly demonstrated that Hg-MIF signals are preserved in Archean and Paleoproterozoic marine shales and massive sulfide deposits. Here, we present evidence that such signals are also preserved in marine shales of mid-Proterozoic age, including the ~1.3 Ga Sulky formation (Dismal Lakes Group, NW Arctic), the ~1.45 Ga Greyson Shale (Belt Basin, Montana), and the ~1.5 Ga Katalsy formation (Kypry Group, Eastern European Platform). We observe that the Greyson shale and shales within the Sulky formation yield negative Hg-MIF with Δ199Hg/Δ201Hg ratios close to 1 and that Kaltasy group sediments

  13. Forensic Stable Isotope Biogeochemistry

    Science.gov (United States)

    Cerling, Thure E.; Barnette, Janet E.; Bowen, Gabriel J.; Chesson, Lesley A.; Ehleringer, James R.; Remien, Christopher H.; Shea, Patrick; Tipple, Brett J.; West, Jason B.

    2016-06-01

    Stable isotopes are being used for forensic science studies, with applications to both natural and manufactured products. In this review we discuss how scientific evidence can be used in the legal context and where the scientific progress of hypothesis revisions can be in tension with the legal expectations of widely used methods for measurements. Although this review is written in the context of US law, many of the considerations of scientific reproducibility and acceptance of relevant scientific data span other legal systems that might apply different legal principles and therefore reach different conclusions. Stable isotopes are used in legal situations for comparing samples for authenticity or evidentiary considerations, in understanding trade patterns of illegal materials, and in understanding the origins of unknown decedents. Isotope evidence is particularly useful when considered in the broad framework of physiochemical processes and in recognizing regional to global patterns found in many materials, including foods and food products, drugs, and humans. Stable isotopes considered in the larger spatial context add an important dimension to forensic science.

  14. Stable isotopes. Applications and production; Les isotopes stables. Applications - production

    Energy Technology Data Exchange (ETDEWEB)

    Goldstein, S.; Louvet, P.; Soulie, E. [eds.

    1994-12-31

    This conference presents 46 communications concerning stable isotope production, utilization and application, grouped in 6 sessions and posters. The various themes are: biological applications (pharmacology, medical diagnosis, metabolism and protein studies, toxicity and response studies, labelled compounds), analysis procedures (NMR analysis for macromolecules, tracer studies), nuclear applications (utilization of stable isotopes in nuclear reactors), biological, physical and chemical applications (mass transfer, mobility, crystallography, isotopic exchange), stable isotope production (ion chromatography, ion cyclotron resonance, cryogenic distillation).

  15. Mother-embryo isotope (δ 15 N, δ 13 C) fractionation and mercury (Hg) transfer in aplacental deep-sea sharks : aplacental shark isotope fractionation and hg

    OpenAIRE

    Le Bourg, B.; Kiszka, J.; Bustamante, P.

    2014-01-01

    Stable carbon (δ13C) and nitrogen (δ15N) isotopic values and total mercury (Hg) concentrations were analysed in muscle and liver of mothers and embryos of two aplacental shark species, Squalus megalops and Centrophorus moluccensis. Embryos of the two species had similar or lower isotopic values than their respective mothers, the only exception being for δ13C, which was higher in the liver of C. moluccensis embryos than in their mothers. Hg concentrations were systematically lower in embryos c...

  16. Nuclear physics and stable isotopes; Physique nucleaire et isotopes stables

    Energy Technology Data Exchange (ETDEWEB)

    Goutte, D. [CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France). Dept. d`Astrophysique, de la Physique des Particules, de la Physique Nucleaire et de l`Instrumentation Associee

    1994-12-31

    The aim of this paper is to show that fundamental research in nuclear physics requires utilization of stable isotopes; stable isotopes are essential as target material since a large quantity of nucleus have to be studied in order to appreciate all the complexity of the nuclear structure, but also as a tool, such as beams, for the same purpose. Examples are given with samarium, tin and germanium isotopes. 7 figs.

  17. Assessing Sources of Human Methylmercury Exposure Using Stable Mercury Isotopes

    DEFF Research Database (Denmark)

    Li, Miling; Sherman, Laura S; Blum, Joel D

    2014-01-01

    Seafood consumption is the primary route of methylmercury (MeHg) exposure for most populations. Inherent uncertainties in dietary survey data point to the need for an empirical tool to confirm exposure sources. We therefore explore the utility of Hg stable isotope ratios in human hair as a new...... method for discerning MeHg exposure sources. We characterized Hg isotope fractionation between humans and their diets using hair samples from Faroese whalers exposed to MeHg predominantly from pilot whales. We observed an increase of 1.75‰ in δ(202)Hg values between pilot whale muscle tissue and Faroese...... whalers' hair but no mass-independent fractionation. We found a similar offset in δ(202)Hg between consumed seafood and hair samples from Gulf of Mexico recreational anglers who are exposed to lower levels of MeHg from a variety of seafood sources. An isotope mixing model was used to estimate individual...

  18. Stable Isotope Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Tissue samples (skin, bone, blood, muscle) are analyzed for stable carbon, stable nitrogen, and stable sulfur analysis. Many samples are used in their entirety for...

  19. Fractionation of Mercury Stable Isotopes during Microbial Methylmercury Production by Iron- and Sulfate-Reducing Bacteria.

    Science.gov (United States)

    Janssen, Sarah E; Schaefer, Jeffra K; Barkay, Tamar; Reinfelder, John R

    2016-08-01

    The biological production of monomethylmercury (MeHg) in soils and sediments is an important factor controlling mercury (Hg) accumulation in aquatic and terrestrial food webs. In this study we examined the fractionation of Hg stable isotopes during Hg methylation in nongrowing cultures of the anaerobic bacteria Geobacter sulfurreducens PCA and Desulfovibrio desulfuricans ND132. Both organisms showed mass-dependent, but no mass-independent fractionation of Hg stable isotopes during Hg methylation. Despite differences in methylation rates, the two bacteria had similar Hg fractionation factors (αr/p = 1.0009 and 1.0011, respectively). Unexpectedly, δ(202)Hg values of MeHg for both organisms were 0.4‰ higher than the value of initial inorganic Hg after about 35% of inorganic Hg had been methylated. These results indicate that a (202)Hg-enriched pool of inorganic Hg was preferentially utilized as a substrate for methylation by these organisms, but that multiple intra- and/or extracellular pools supplied inorganic Hg for biological methylation. Understanding the controls of the Hg stable isotopic composition of microbially produced MeHg is important to identifying bioavailable Hg in natural systems and the interpretation of Hg stable isotopes in aquatic food webs.

  20. Natural Hg isotopic composition of different Hg compounds in mammal tissues as a proxy for in vivo breakdown of toxic methylmercury.

    Science.gov (United States)

    Perrot, Vincent; Masbou, Jeremy; Pastukhov, Mikhail V; Epov, Vladimir N; Point, David; Bérail, Sylvain; Becker, Paul R; Sonke, Jeroen E; Amouroux, David

    2016-02-01

    In the last decade, specific attention has been paid to total mercury (HgT) stable isotopic composition, especially in natural samples such as aquatic organisms, due to its potential to track the cycle of this toxic element in the environment. Here, we investigated Hg Compound Specific stable Isotopic Composition (CSIC) of natural inorganic Hg (iHg) and methylmercury (MMHg) in various tissues of aquatic mammals (Beluga whale from the Arctic marine environment and seals from the freshwater lake Baikal, Russia). In seals' organs the variation in mass dependent fractionation (MDF, δ(202)Hg) for total Hg was significantly correlated to the respective fraction of iHg and MMHg compounds, with MMHg being enriched by ∼ 3‰ in heavier isotopes relative to iHg. On the other hand, we observe insignificant variation in Hg mass independent isotope fractionation (MIF, Δ(199)Hg) among iHg and MMHg in all organs for the same mammal species and MMHg in prey items. MIF signatures suggest that both MMHg and iHg in aquatic mammals have the same origin (i.e., MMHg from food), and are representative of Hg photochemistry in the water column of the mammal ecosystem. MDF signatures of Hg compounds indicate that MMHg is demethylated in vivo before being stored in the muscle, and the iHg formed is stored in the liver, and to a lesser extent in the kidney, before excretion. Thus, Hg CSIC analysis in mammals can be a powerful tool for tracing the metabolic response to Hg exposure.

  1. Investigation of Hg uptake and transport between paddy soil and rice seeds combining Hg isotopic composition and speciation

    Directory of Open Access Journals (Sweden)

    C. Feng

    2016-02-01

    Full Text Available Abstract Human consumption of rice constitutes a potential toxicological risk in mercury (Hg polluted areas such as Hg mining regions in China. It is recognized to be an important source of Hg for the local human diet considering the efficient bioaccumulation of methylmercury (MeHg in rice seed. To assess Hg sources and uptake pathways to the rice plants, Hg speciation and isotopic composition were investigated in rice seeds and their corresponding paddy soils from different locations within the Wanshan Hg mining area (Guizhou Province, China. A large variation of Hg speciation is observed in rice seeds and paddy soils irrespective of the sampling location. Mass dependent fractionation (MDF of Hg in rice seeds differs by up to ∼4.0 ‰ in δ202Hg values, while mass independent fractionation (MIF of Hg isotopes remains constant (Δ199Hg ∼ 0‰. Hg isotopic composition in rice seeds covaries with that of paddy soils but exhibits lighter isotopic signature (δ202Hg. Such isotopic offset is mainly attributed to plant uptake and translocation processes. Also, seeds containing higher MeHg (MeHg/total Hg > 50% have significantly heavier Hg isotopes suggesting that MeHg uptake and transport to the seed in such rice plants is facilitated compared to inorganic Hg.

  2. Properties of Light Hg, Pb and Po Isotopes

    CERN Document Server

    Muntian, I

    2003-01-01

    Quality of mass description for three different theoretical mass models is studied. Masses and deformations for Po, Pb and Hg isotopes are compared with experimental data. Gap in the proton single particle energy spectrum is discussed.

  3. Bayesian stable isotope mixing models

    Science.gov (United States)

    In this paper we review recent advances in Stable Isotope Mixing Models (SIMMs) and place them into an over-arching Bayesian statistical framework which allows for several useful extensions. SIMMs are used to quantify the proportional contributions of various sources to a mixtur...

  4. Paleoproxies: Heavy Stable Isotope Perspectives

    Science.gov (United States)

    Nagler, T. F.; Hippler, D.; Siebert, C.; Kramers, J. D.

    2002-12-01

    Recent advances in isotope ratio mass spectrometry, namely multiple collector ICP-MS and refined TIMS techniques, will significantly enhance the ability to measure heavy stable isotope fractionation, which will lead to the development of a wide array of process-identifying (bio)-geochemical tools. Thus far research in this area is not easily assessable to scientists outside the isotope field. This is due to the fact that analyzing heavy stable isotopes does not provide routine numbers which are per se true (the preciser the truer) but is still a highly experimental field. On the other hand resolving earth science problems requires specialists familiar with the environment being studied. So what is in there for paleoceanographers? In a first order approach, relating isotope variations to physical processes is straightforward. A prominent example are oxygen isotope variations with temperature. The total geological signal is of course far more complicated. At low temperatures, heavy stable isotopes variations have been reported for e.g. Ca, Cr, Fe, Cu, Zn, Mo and Tl. Fractionation mechanisms and physical parameters responsible for the observed variations are not yet resolved for most elements. Significant equilibrium isotope fractionation is expected from redox reactions of transition metals. However a difference in coordination number between two coexisting speciations of an element in the same oxidation state can also cause fractionation. Protonation of dissolved Mo is one case currently discussed. For paleoceanography studies, a principal distinction between transition metals essential for life (V to Zn plus Mo) or not will be helpful. In case of the former group, distinction between biogenic and abiogenic isotope fractionation will remain an important issue. For example, abiotic Fe redox reactions result in isotope fractionations indistinguishable in direction and magnitude from microbial effects. Only a combination of different stable isotope systems bears the

  5. Mercury Stable Isotopic Composition of Monomethylmercury in Estuarine Sediments and Pure Cultures of Mercury Methylating Bacteria

    Science.gov (United States)

    Janssen, S.; Johnson, M. W.; Barkay, T.; Blum, J. D.; Reinfelder, J. R.

    2014-12-01

    Tracking monomethylmercury (MeHg) from its source in soils and sediments through various environmental compartments and transformations is critical to understanding its accumulation in aquatic and terrestrial food webs. Advances in the field of mercury (Hg) stable isotopes have allowed for the tracking of discrete Hg sources and the examination of photochemical and bacterial transformations. Despite analytical advances, measuring the Hg stable isotopic signature of MeHg in environmental samples or laboratory experiments remains challenging due to difficulties in the quantitative separation of MeHg from complex matrices with high concentrations of inorganic Hg. To address these challenges, we have developed a MeHg isolation method for sediments and bacterial cultures which involves separation by gas chromatography. The MeHg eluting from the GC is passed through a pyrolysis column and purged onto a gold amalgam trap which is then desorbed into a final oxidizing solution. A MeHg reference standard carried through our separation process retained its isotopic composition within 0.02 ‰ for δ202Hg, and for native estuarine sediments, MeHg recoveries were 80% to 100%. For sediment samples from the Hackensack and Passaic Rivers (New Jersey, USA), δ202Hg values for MeHg varied from -1.2 to +0.58 ‰ (relative to SRM 3133) and for individual samples were significantly different from that of total Hg (-0.38 ± 0.06 ‰). No mass independent fractionation was observed in MeHg or total Hg from these sediments. Pure cultures of Geobacter sulfurreducens, grown under fermentative conditions showed preferential enrichment of lighter isotopes (lower δ202Hg) during Hg methylation. The Hg stable isotope signatures of MeHg in sediments and laboratory methylation experiments will be discussed in the context of the formation and degradation of MeHg in the environment and the bioaccumulation of MeHg in estuarine food webs.

  6. Final Project Report: "Exploratory Research: Mercury Stable Isotopes as Indicators of the Biogeochemical Cycling of Mercury"

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, Thomas M

    2012-08-01

    This is the final project report for award DE-SC0005351, which supported the research project "Exploratory Research: Mercury Stable Isotopes as Indicators of the Biogeochemical Cycling of Mercury. "This exploratory project investigated the use of mercury (Hg) stable isotope measurements as a new approach to study how Hg moves and changes its chemical form in environmental systems, with particular focus on the East Fork of Poplar Creek (EFPC) near the DOE Y-12 plant (a Hg contamination source). This study developed analytical methods and collected pilot data that have set the stage for more detailed studies and have begun to provide insights into Hg movement and chemical changes. The overall Hg stable isotope approach was effective. The Hg isotope analysis methods yielded high-precision measurements of the sediment, water, and fish samples analyzed; quality control measures demonstrated the precision. The pilot data show that the 202Hg/198Hg, 199Hg/198Hg, and 201Hg/198Hg isotope ratios vary in this system. 202Hg/198Hg ratios of the Hg released from the Y-12 plant are relatively high, and those of the regional Hg background in soils and river sediments are significantly lower. Unfortunately, 202Hg/198Hg differences that might have been useful to distinguish early Hg releases from later releases were not observed. However, 202Hg/198Hg ratios in sediments do provide insights into chemical transformations that may occur as Hg moves through the system. Furthermore, 199Hg/198Hg and 201Hg/198Hg ratio analyses of fish tissues indicate that the effects of sunlight-driven chemical reactions on the Hg that eventually ends up in EFPC fish are measureable, but small. These results provide a starting point for a more detailed study (already begun at Univ. of Michigan) that will continue Hg isotope ratio work aimed at improving understanding of how Hg moves, changes chemically, and does or does not take on more highly toxic forms in the Oak Ridge area. This work also benefits

  7. Metal stable isotope signatures as tracers in environmental geochemistry.

    Science.gov (United States)

    Wiederhold, Jan G

    2015-03-03

    The biogeochemical cycling of metals in natural systems is often accompanied by stable isotope fractionation which can now be measured due to recent analytical advances. In consequence, a new research field has emerged over the last two decades, complementing the traditional stable isotope systems (H, C, O, N, S) with many more elements across the periodic table (Li, B, Mg, Si, Cl, Ca, Ti, V, Cr, Fe, Ni, Cu, Zn, Ge, Se, Br, Sr, Mo, Ag, Cd, Sn, Sb, Te, Ba, W, Pt, Hg, Tl, U) which are being explored and potentially applicable as novel geochemical tracers. This review presents the application of metal stable isotopes as source and process tracers in environmental studies, in particular by using mixing and Rayleigh model approaches. The most important concepts of mass-dependent and mass-independent metal stable isotope fractionation are introduced, and the extent of natural isotopic variations for different elements is compared. A particular focus lies on a discussion of processes (redox transformations, complexation, sorption, precipitation, dissolution, evaporation, diffusion, biological cycling) which are able to induce metal stable isotope fractionation in environmental systems. Additionally, the usefulness and limitations of metal stable isotope signatures as tracers in environmental geochemistry are discussed and future perspectives presented.

  8. Historical variations of mercury stable isotope ratios in Arctic glacier firn and ice cores

    Science.gov (United States)

    Zdanowicz, C. M.; Krümmel, E. M.; Poulain, A. J.; Yumvihoze, E.; Chen, J.; Å trok, M.; Scheer, M.; Hintelmann, H.

    2016-09-01

    The concentration and isotopic composition of mercury (Hg) were determined in glacier core samples from Canadian Arctic ice caps dating from preindustrial to recent time (early 21st century). Mean Hg levels increased from ≤ 0.2 ng L-1 in preindustrial time to ~0.8-1.2 ng L-1 in the modern industrial era (last ~200 years). Hg accumulated on Arctic ice caps has Δ199Hg and Δ201Hg that are higher (~ -1 to 2.9‰) than previously reported for Arctic snow impacted by atmospheric Hg depletion events (mostly < -1‰), suggesting that these events contribute little to Hg accumulation on ice caps. The range of δ202Hg, Δ199Hg, and Δ201Hg in glacier cores overlaps with that of Arctic Hg0(g) and of seawater in Baffin Bay and also with that of midlatitude precipitation and industrial Hg sources, including coal and Hg ores. A core from Agassiz ice cap (80.7°N) shows a ~ +1‰ shift in δ202Hg over the nineteenth to twentieth centuries that could reflect changes in the isotopic composition of the atmospheric Hg pool in the High Arctic in response to growing industrial emissions at lower latitudes. This study is the first ever to report on historical variations of Hg stable isotope ratios in Arctic ice cores. Results could help constrain future modeling efforts of the global Hg biogeochemical cycle and the atmosphere's response to changing Hg emissions, past and future.

  9. Historical (1850–2010 mercury stable isotope inventory from anthropogenic sources to the atmosphere

    Directory of Open Access Journals (Sweden)

    Ruoyu Sun

    2016-02-01

    Full Text Available Abstract Mercury (Hg stable isotopes provide a new tool to trace the biogeochemical cycle of Hg. An inventory of the isotopic composition of historical anthropogenic Hg emissions is important to understand sources and post-emission transformations of Hg. We build on existing global inventories of anthropogenic Hg emissions to the atmosphere to develop the first corresponding historical Hg isotope inventories for total Hg (THg and three Hg species: gaseous elemental Hg (GEM, gaseous oxidized Hg (GOM and particulate-bound Hg (PBM. We compile δ202Hg and Δ199Hg of major Hg emissions source materials. Where possible, δ202Hg and Δ199Hg values in emissions are corrected for the mass dependent Hg isotope fractionation during industrial processing. The framework and Hg isotope inventories can be updated and improved as new data become available. Simulated THg emissions from all sectors between 1850s and 2010s generally show an increasing trend (−1.1‰ to −0.7‰ for δ202Hg, and a stable trend (−0.02‰ to −0.04‰ for Δ199Hg. Δ200Hg are near-zero in source materials and therefore emissions. The δ202Hg trend generally reflects a shift of historically dominant Hg emissions from 19th century Hg mining and liquid Hg0 uses in Au/Ag refining to 20th century coal combustion and non-ferrous metal production. The historical δ202Hg and Δ199Hg curves of GEM closely follow those of THg. The δ202Hg curves of GOM and PBM show no trends. Δ199Hg values for both GOM and PBM decrease from the 1850s to 1950s by ∼0.1‰, and then gradually rebound towards the 2010s. Our updated δ202Hg values (−0.76 ± 0.11 ‰, 1SD, n=9 of bulk emissions from passively degassing volcanoes overlap with δ202Hg of present-day anthropogenic THg emissions.

  10. The trend of stable isotope separation

    Energy Technology Data Exchange (ETDEWEB)

    Yonekawa, Shigeru [Power Reactor and Nuclear Fuel Development Corp., Kamisaibara, Okayama (Japan). Ningyo Toge Works; Aoki, Eiji; Yato, Yumio

    1994-12-01

    Recently, stable isotopes are used in the field of medical science, nuclear physics, environmental science and agriculture. This report reviews the present status of stable isotope enrichment in ORNL, Urenco, Russia and PNC. Further the utilization method of the stable isotopes in the field of medical science, nuclear power and material science are described, and the application possibility of Laser separation method and Gas Centrifuge method are estimated. There are many overseas actual results of stable isotope separation with Gas Centrifuge method, therefore this method is possible enough in principle. (author).

  11. Mercury stable isotope signatures of world coal deposits and historical coal combustion emissions.

    Science.gov (United States)

    Sun, Ruoyu; Sonke, Jeroen E; Heimbürger, Lars-Eric; Belkin, Harvey E; Liu, Guijian; Shome, Debasish; Cukrowska, Ewa; Liousse, Catherine; Pokrovsky, Oleg S; Streets, David G

    2014-07-01

    Mercury (Hg) emissions from coal combustion contribute approximately half of anthropogenic Hg emissions to the atmosphere. With the implementation of the first legally binding UNEP treaty aimed at reducing anthropogenic Hg emissions, the identification and traceability of Hg emissions from different countries/regions are critically important. Here, we present a comprehensive world coal Hg stable isotope database including 108 new coal samples from major coal-producing deposits in South Africa, China, Europe, India, Indonesia, Mongolia, former USSR, and the U.S. A 4.7‰ range in δ(202)Hg (-3.9 to 0.8‰) and a 1‰ range in Δ(199)Hg (-0.6 to 0.4‰) are observed. Fourteen (p coal Hg emissions tracing. A revised coal combustion Hg isotope fractionation model is presented, and suggests that gaseous elemental coal Hg emissions are enriched in the heavier Hg isotopes relative to oxidized forms of emitted Hg. The model explains to first order the published δ(202)Hg observations on near-field Hg deposition from a power plant and global scale atmospheric gaseous Hg. Yet, model uncertainties appear too large at present to permit straightforward Hg isotope source identification of atmospheric forms of Hg. Finally, global historical (1850-2008) coal Hg isotope emission curves were modeled and indicate modern-day mean δ(202)Hg and Δ(199)Hg values for bulk coal emissions of -1.2 ± 0.5‰ (1SD) and 0.05 ± 0.06‰ (1SD).

  12. Substitution of stable isotopes in Chlorella

    Science.gov (United States)

    Flaumenhaft, E.; Katz, J. J.; Uphaus, R. A.

    1969-01-01

    Replacement of biologically important isotopes in the alga Chlorella by corresponding heavier stable isotopes produces increasingly greater deviations from the normal cell size and changes the quality and distribution of certain cellular components. The usefulness of isotopically altered organisms increases interest in the study of such permuted organisms.

  13. Modelling stable water isotopes: Status and perspectives

    Directory of Open Access Journals (Sweden)

    Werner M.

    2010-12-01

    Full Text Available Modelling of stable water isotopes H2 18O and HDO within various parts of the Earth’s hydrological cycle has clearly improved our understanding of the interplay between climatic variations and related isotope fractionation processes. In this article key principles and major research results of stable water isotope modelling studies are described. Emphasis is put on research work using explicit isotope diagnostics within general circulation models as this highly complex model setup bears many resemblances with studies using simpler isotope modelling approaches.

  14. Applications of stable isotopes in clinical pharmacology

    NARCIS (Netherlands)

    Schellekens, Reinout C A; Stellaard, Frans; Woerdenbag, Herman J; Frijlink, Henderik W; Kosterink, Jos G W

    2011-01-01

    This review aims to present an overview of the application of stable isotope technology in clinical pharmacology. Three main categories of stable isotope technology can be distinguished in clinical pharmacology. Firstly, it is applied in the assessment of drug pharmacology to determine the pharmacok

  15. Applications of stable isotopes in clinical pharmacology

    NARCIS (Netherlands)

    Schellekens, Reinout C A; Stellaard, Frans; Woerdenbag, Herman J; Frijlink, Henderik W; Kosterink, Jos G W

    2011-01-01

    This review aims to present an overview of the application of stable isotope technology in clinical pharmacology. Three main categories of stable isotope technology can be distinguished in clinical pharmacology. Firstly, it is applied in the assessment of drug pharmacology to determine the

  16. Isotopic Composition of Gaseous Elemental Mercury (Hg0) at Various Sites in Japan

    Science.gov (United States)

    Yamakawa, A.; Moriya, K.; Yoshinaga, J.

    2015-12-01

    Mercury (Hg) is a toxic heavy metal, which exists in various chemical forms in the environmental system. In the atmosphere, Hg exists in three forms (Hg0(g), Hg+2(g), and Hg(p)). Hg0(g) is the dominant species of atmospheric Hg, accounting for >95% of the total Hg in the atmosphere. Because Hg0(g) is highly volatile and has limited solubility in water, it cannot be easily removed by wet or dry deposition processes. Therefore, the residence time of Hg0(g) in the atmosphere is relatively long (1 to 2 years), allowing long-range transport from mercury emission source(s). Conversely, Hg+2(g) and Hg(p) are effectively removed from the atmosphere through wet and dry depositions. The determination of mercury source attribution using quantitative data is challenging because Hg0(g) may be deposited on an area upon oxidation to Hg+2(g) and associated with aerosols and particulates to form Hg(p) while the global cycling of Hg0(g). Over the last decade, the development of analytical methods of highly precise Hg isotopic measurements demonstrated mass-dependent fractionation (MDF) and mass-independent fractionation (MIF) of Hg isotopes in environmental samples. For instance, MDF of Hg isotopes is thought to occur during various natural and industrial Hg transformations. MIF of Hg isotopes is observed during abiotic reduction, photochemical and non-photochemical, and physical and chemical processes. Such processes lead to differences in the Hg isotopic composition of different emission sources, both natural and anthropogenic, and atmospheric processes (i.e., transportation, oxidation/reduction, deposition, and reemission). Therefore, Hg isotopic compositions could be used to trace the sources and processes of atmospheric Hg. For securing the reliability and accuracy of atmospheric Hg isotope data, the methods of collection, pretreatment, and isotopic measurement for Hg0(g) were developed to obtain high recovery yield of samples with no Hg isotopic fractionation during each

  17. Assessment of mercury exposure among small-scale gold miners using mercury stable isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Sherman, Laura S., E-mail: lsaylors@umich.edu [University of Michigan, Department of Earth and Environmental Sciences, 1100 North University Avenue, Ann Arbor, MI 48109 (United States); Blum, Joel D. [University of Michigan, Department of Earth and Environmental Sciences, 1100 North University Avenue, Ann Arbor, MI 48109 (United States); Basu, Niladri [McGill University, Faculty of Agricultural and Environmental Sciences, 21,111 Lakeshore Road, Ste. Anne de Bellevue, Quebec, Canada H9X3V9 (Canada); Rajaee, Mozhgon [University of Michigan, Department of Environmental Health Sciences, 1415 Washington Heights, Ann Arbor, MI 48109 (United States); Evers, David C.; Buck, David G. [Biodiversity Research Institute, 19 Flaggy Meadow Road, Gorham, ME 04038 (United States); Petrlik, Jindrich [Arnika Association, Chlumova 17, Prague 3 (Czech Republic); DiGangi, Joseph [IPEN, Box 7256, SE-402 35 Gothenburg (Sweden)

    2015-02-15

    Total mercury (Hg) concentrations in hair and urine are often used as biomarkers of exposure to fish-derived methylmercury (MeHg) and gaseous elemental Hg, respectively. We used Hg stable isotopes to assess the validity of these biomarkers among small-scale gold mining populations in Ghana and Indonesia. Urine from Ghanaian miners displayed similar Δ{sup 199}Hg values to Hg derived from ore deposits (mean urine Δ{sup 199}Hg=0.01‰, n=6). This suggests that urine total Hg concentrations accurately reflect exposure to inorganic Hg among this population. Hair samples from Ghanaian miners displayed low positive Δ{sup 199}Hg values (0.23–0.55‰, n=6) and low percentages of total Hg as MeHg (7.6–29%, n=7). These data suggest that the majority of the Hg in these miners' hair samples is exogenously adsorbed inorganic Hg and not fish-derived MeHg. Hair samples from Indonesian gold miners who eat fish daily displayed a wider range of positive Δ{sup 199}Hg values (0.21–1.32‰, n=5) and percentages of total Hg as MeHg (32–72%, n=4). This suggests that total Hg in the hair samples from Indonesian gold miners is likely a mixture of ingested fish MeHg and exogenously adsorbed inorganic Hg. Based on data from both populations, we suggest that total Hg concentrations in hair samples from small-scale gold miners likely overestimate exposure to MeHg from fish consumption. - Highlights: • Mercury isotopes were measured in hair and urine from small-scale gold miners. • Mercury isotopes indicate that Hg in urine comes from mining activity. • Mercury isotopes suggest Hg in hair is a mixture of fish MeHg and inorganic Hg. • A large percentage of Hg in miner’s hair is released during amalgam burning and adsorbed.

  18. Carbon Stable Isotope Analysis of Methylmercury Toxin in Biological Materials by Gas Chromatography Isotope Ratio Mass Spectrometry.

    Science.gov (United States)

    Masbou, Jeremy; Point, David; Guillou, Gaël; Sonke, Jeroen E; Lebreton, Benoit; Richard, Pierre

    2015-12-01

    A critical component of the biogeochemical cycle of mercury (Hg) is the transformation of inorganic Hg to neurotoxic monomethylmercury (CH3Hg). Humans are exposed to CH3Hg by consuming marine fish, yet the origin of CH3Hg in fish is a topic of debate. The carbon stable isotopic composition (δ(13)C) embedded in the methyl group of CH3Hg remains unexplored. This new isotopic information at the molecular level is thought to represent a new proxy to trace the carbon source at the origin of CH3Hg. Here, we present a compound-specific stable isotope analysis (CSIA) technique for the determination of the δ(13)C value of CH3Hg in biological samples by gas chromatography combustion isotope ratio mass spectrometry analysis (GC-C-IRMS). The method consists first of calibrating a CH3Hg standard solution for δ(13)C CSIA. This was achieved by comparing three independent approaches consisting of the derivatization and halogenation of the CH3Hg standard solution. The determination of δ(13)C(CH3Hg) values on natural biological samples was performed by combining a CH3Hg selective extraction, purification, and halogenation followed by GC-C-IRMS analysis. Reference δ(13)C values were established for a tuna fish certified material (ERM-CE464) originating from the Adriatic Sea (δ(13)C(CH3Hg) = -22.1 ± 1.5‰, ± 2 SD). This value is similar to the δ(13)C value of marine algal-derived particulate organic carbon (δ(13)CPOC = -21‰).

  19. Linking mercury, carbon, and nitrogen stable isotopes in Tibetan biota: Implications for using mercury stable isotopes as source tracers.

    Science.gov (United States)

    Xu, Xiaoyu; Zhang, Qianggong; Wang, Wen-Xiong

    2016-05-06

    Tibetan Plateau is located at a mountain region isolated from direct anthropogenic sources. Mercury concentrations and stable isotopes of carbon, nitrogen, and mercury were analyzed in sediment and biota for Nam Co and Yamdrok Lake. Biotic mercury concentrations and high food web magnification factors suggested that Tibetan Plateau is no longer a pristine site. The primary source of methylmercury was microbial production in local sediment despite the lack of direct methylmercury input. Strong ultraviolet intensity led to extensive photochemical reactions and up to 65% of methylmercury in water was photo-demethylated before entering the food webs. Biota displayed very high Δ(199)Hg signatures, with some highest value (8.6%) ever in living organisms. The δ(202)Hg and Δ(199)Hg in sediment and biotic samples increased with trophic positions (δ(15)N) and %methylmercury. Fish total length closely correlated to δ(13)C and Δ(199)Hg values due to dissimilar carbon sources and methylmercury pools in different living waters. This is the first mercury isotope study on high altitude lake ecosystems that demonstrated specific isotope fractionations of mercury under extreme environmental conditions.

  20. Quantitative microbial ecology through stable isotope probing.

    Science.gov (United States)

    Hungate, Bruce A; Mau, Rebecca L; Schwartz, Egbert; Caporaso, J Gregory; Dijkstra, Paul; van Gestel, Natasja; Koch, Benjamin J; Liu, Cindy M; McHugh, Theresa A; Marks, Jane C; Morrissey, Ember M; Price, Lance B

    2015-11-01

    Bacteria grow and transform elements at different rates, and as yet, quantifying this variation in the environment is difficult. Determining isotope enrichment with fine taxonomic resolution after exposure to isotope tracers could help, but there are few suitable techniques. We propose a modification to stable isotope probing (SIP) that enables the isotopic composition of DNA from individual bacterial taxa after exposure to isotope tracers to be determined. In our modification, after isopycnic centrifugation, DNA is collected in multiple density fractions, and each fraction is sequenced separately. Taxon-specific density curves are produced for labeled and nonlabeled treatments, from which the shift in density for each individual taxon in response to isotope labeling is calculated. Expressing each taxon's density shift relative to that taxon's density measured without isotope enrichment accounts for the influence of nucleic acid composition on density and isolates the influence of isotope tracer assimilation. The shift in density translates quantitatively to isotopic enrichment. Because this revision to SIP allows quantitative measurements of isotope enrichment, we propose to call it quantitative stable isotope probing (qSIP). We demonstrated qSIP using soil incubations, in which soil bacteria exhibited strong taxonomic variations in (18)O and (13)C composition after exposure to [(18)O]water or [(13)C]glucose. The addition of glucose increased the assimilation of (18)O into DNA from [(18)O]water. However, the increase in (18)O assimilation was greater than expected based on utilization of glucose-derived carbon alone, because the addition of glucose indirectly stimulated bacteria to utilize other substrates for growth. This example illustrates the benefit of a quantitative approach to stable isotope probing.

  1. Measuring In Vivo Ureagenesis With Stable Isotopes

    Science.gov (United States)

    Yudkoff, Marc; Mew, Nicholas Ah; Daikhin, Yevgeny; Horyn, Oksana; Nissim, Ilana; Nissim, Itzhak; Payan, Irma; Tuchman, Mendel

    2010-01-01

    Stable isotopes have been an invaluable adjunct to biomedical research for more than 70 years. Indeed, the isotopic approach has revolutionized our understanding of metabolism, revealing it to be an intensely dynamic process characterized by an unending cycle of synthesis and degradation. Isotopic studies have taught us that the urea cycle is intrinsic to such dynamism, since it affords a capacious mechanism by which to eliminate waste nitrogen when rates of protein degradation (or dietary protein intake) are especially high. Isotopes have enabled an appreciation of the degree to which ureagenesis is compromised in patients with urea cycle defects. Indeed, isotopic studies of urea cycle flux correlate well with the severity of cognitive impairment in these patients. Finally, the use of isotopes affords an ideal tool with which to gauge the efficacy of therapeutic interventions to augment residual flux through the cycle. PMID:20338795

  2. Stable Oxygen-18 and Deuterium Isotopes

    DEFF Research Database (Denmark)

    Müller, Sascha

    The application of stable Oxygen-18 (18O) and Deuterium (2H) isotopes, as a tracer for fluxes between different compartments of the water cycle was subject of the present PhD-thesis. During a three year period, temporal data from a wide range of water cycle constituents was collected from...... the Skjern River catchment, Denmark. The presented applications focused on studying the isotopic 'input signal' to the hydrosphere in the form of precipitation, the isotopic 'output signal' with its related dynamic processes at a coastal saltwater-freshwater interface (groundwater isotopes) and the temporal...... development within a given lowland headwater catchment (stream water isotopes). Based on our investigations on the precipitation isotopic composition a local meteoric water line (LMWL) was constructed and expressed as: δ2H=7.4 δ18O + 5.36‰. Moreover, we showed that under maritime temperature climate influence...

  3. Stable isotope utilization methodology; Methodologie de l`emploi des isotopes stables

    Energy Technology Data Exchange (ETDEWEB)

    Roth, E. [Conservatoire National des Arts et Metiers (CNAM), 75 - Paris (France)

    1994-12-31

    The various applications of stable isotope utilization are reviewed, as a function of their specific properties: poly-isotopic abundance modification is used for tracer applications; the accurate measurement of the stable isotope abundance may be applied to isotopic dilution for ultra-trace measurement, physical constant determination, fluid volume and concentration measurement; isotopic effects, such as reaction equilibrium differences are used for separation and identification of molecule active centers (pharmacology, paleoclimatology, hydrogeological studies) while reaction rate differences (competitive and non competitive methods) are used for the study of reaction mechanisms, such as enzymatic reactions. Analysis techniques (mass spectrometry, nuclear magnetic resonance, optical methods) are reviewed. 2 figs., 18 refs.

  4. Stable isotopes in Lithuanian bioarcheological material

    Science.gov (United States)

    Skipityte, Raminta; Jankauskas, Rimantas; Remeikis, Vidmantas

    2015-04-01

    Investigation of bioarcheological material of ancient human populations allows us to understand the subsistence behavior associated with various adaptations to the environment. Feeding habits are essential to the survival and growth of ancient populations. Stable isotope analysis is accepted tool in paleodiet (Schutkowski et al, 1999) and paleoenvironmental (Zernitskaya et al, 2014) studies. However, stable isotopes can be useful not only in investigating human feeding habits but also in describing social and cultural structure of the past populations (Le Huray and Schutkowski, 2005). Only few stable isotope investigations have been performed before in Lithuanian region suggesting a quite uniform diet between males and females and protein intake from freshwater fish and animal protein. Previously, stable isotope analysis has only been used to study a Stone Age population however, more recently studies have been conducted on Iron Age and Late medieval samples (Jacobs et al, 2009). Anyway, there was a need for more precise examination. Stable isotope analysis were performed on human bone collagen and apatite samples in this study. Data represented various ages (from 5-7th cent. to 18th cent.). Stable carbon and nitrogen isotope analysis on medieval populations indicated that individuals in studied sites in Lithuania were almost exclusively consuming C3 plants, C3 fed terrestrial animals, and some freshwater resources. Current investigation demonstrated social differences between elites and country people and is promising in paleodietary and daily life reconstruction. Acknowledgement I thank prof. dr. G. Grupe, Director of the Anthropological and Palaeoanatomical State Collection in Munich for providing the opportunity to work in her laboratory. The part of this work was funded by DAAD. Antanaitis-Jacobs, Indre, et al. "Diet in early Lithuanian prehistory and the new stable isotope evidence." Archaeologia Baltica 12 (2009): 12-30. Le Huray, Jonathan D., and Holger

  5. Stable isotope dilution assays in mycotoxin analysis

    Energy Technology Data Exchange (ETDEWEB)

    Rychlik, Michael; Asam, Stefan [Universitaet Muenchen, Lehrstuhl fuer Lebensmittelchemie der Technischen, Garching (Germany)

    2008-01-15

    The principle and applications of stable isotope dilution assays (SIDAs) in mycotoxin analysis are critically reviewed. The general section includes historical aspects of SIDAs, the prerequisites and limitations of the use of stable isotopically labelled internal standards, and possible calibration procedures. In the application section actual SIDAs for the analysis of trichothecenes, zearalenone, fumonisins, patulin, and ochratoxin A are presented. The syntheses and availability of labelled mycotoxins for use as internal standards is reviewed and specific advances in food analysis and toxicology are demonstrated. The review indicates that LC-MS applications, in particular, require the use of stable isotopically labelled standards to compensate for losses during clean-up and for discrimination due to ion suppression. As the commercial availability of these compounds continues to increase, SIDAs can be expected to find expanding use in mycotoxin analysis. (orig.)

  6. Stable isotope dilution assays in mycotoxin analysis.

    Science.gov (United States)

    Rychlik, Michael; Asam, Stefan

    2008-01-01

    The principle and applications of stable isotope dilution assays (SIDAs) in mycotoxin analysis are critically reviewed. The general section includes historical aspects of SIDAs, the prerequisites and limitations of the use of stable isotopically labelled internal standards, and possible calibration procedures. In the application section actual SIDAs for the analysis of trichothecenes, zearalenone, fumonisins, patulin, and ochratoxin A are presented. The syntheses and availability of labelled mycotoxins for use as internal standards is reviewed and specific advances in food analysis and toxicology are demonstrated. The review indicates that LC-MS applications, in particular, require the use of stable isotopically labelled standards to compensate for losses during clean-up and for discrimination due to ion suppression. As the commercial availability of these compounds continues to increase, SIDAs can be expected to find expanding use in mycotoxin analysis.

  7. Exploiting Stable Mercury Isotopic Analysis to Differentiate between Mercury Sources: Gold Mining vs. Land-Use Change (Invited)

    Science.gov (United States)

    Bergquist, B. A.; Adler Miserendino, R. A.; Guimarães, J. R.; Veiga, M.; Velasquez-López, P.; Lees, P. S.; Thibodeau, A. M.; Fernandez, L. E.

    2013-12-01

    In parts of the developing world, mercury (Hg) is used to extract gold by amalgamation during artisanal and small-scale gold mining (ASGM) and this can lead to contamination of downstream aquatic ecosystems. Differentiation between Hg from ASGM and from other sources of Hg, such as increased erosion from land cover and land use change (LCLUC), is challenging and has lead to heated debates over the dominant sources of elevated Hg in some ecosystems. Here, stable Hg isotopic analysis was applied in two aquatic ecosystems in South America: (1) the Amazonian aquatic ecosystem of Amapá, Brazil downstream of artisanal gold mining (AGM) and (2) the Puyango-Tumbes River ecosystem downstream of Portovelo-Zaruma, Ecuador, a large mining area where both AGM and small-scale gold mining (SGM) are in operation. The Hg isotopic analyses from Amapá, Brazil, do not support AGM as the source of elevated Hg in the downstream aquatic ecosystem. Instead, Hg isotopes are most consistent with the elevated Hg being from preferential migration of Hg from soil erosion, which is likely associated with land use change. Although soils are regarded as Hg sinks in the global Hg cycle, this work suggests that LCLUC can disrupt Hg stores with significant ecological consequences. In contrast in the Southwestern Andean region of Ecuador and Peru, analysis of Hg isotopes and other toxic metals (i.e., Pb, Zn, Cu), which are associated with the larger scale mining and cyanide used during SGM, demonstrate Hg used during gold mining is the predominant source of Hg downstream and can be traced far from the dominant mining area. Although it has been speculated that Hg from SGM in Ecuador was not that mobile or that Hg far downstream of SGM processing plants was from erosion due to LCLUC or from AGM taking place downstream, the isotopically heavy signature of Hg used during gold mining and elevated other metal concentrations were observed ~120 km downstream of Portovelo-Zaruma. Mercury isotopes appear

  8. Mother-embryo isotope (δ15N, δ13C) fractionation and mercury (Hg) transfer in aplacental deep-water sharks

    OpenAIRE

    Le Bourg, Baptiste; Kiszka, Jeremy; Bustamante, Paco

    2014-01-01

    International audience; Stable carbon (δ13C) and nitrogen (δ15N) isotopic values and total mercury (Hg) concentrations were analysed in muscle and liver of mothers and embryos of two aplacental shark species, Squalus megalops and Centrophorus moluccensis. Embryos of the two species had similar or lower isotopic values than their respective mothers, the only exception being for δ13C, which was higher in the liver of C. moluccensis embryos than in their mothers. Hg concentrations were systemati...

  9. The production of stable isotopes in Spain

    Energy Technology Data Exchange (ETDEWEB)

    Urgel, M.; Iglesias, J.; Casas, J.; Saviron, J. M.; Quintanilla, M.

    1965-07-01

    The activities developed in the field of the production of stable isotopes by means of ion-exchange chromatography and thermal diffusion techniques are reported. The first method was used to study the separation of the nitrogen and boron isotopes, whereby the separation factor was determined by the break through method. Values ranging from 1,028 to 1,022 were obtained for the separation factor of nitrogen by using ammonium hydroxide solutions while the corresponding values as obtained for boron amounted to 1,035-1,027 using boric acid solutions. Using ammonium chloride or acetate and sodium borate, respectively, resulted in the obtention of values for the separation factor approaching unity. The isotopic separation has been carried out according to the method of development by displacement. The separation of the isotopes of the noble gases, oxygen, nitrogen and carbon has been accomplished resorting to the method of thermal diffusion. (Author) 16 refs.

  10. On the progress in stable isotope separation

    Energy Technology Data Exchange (ETDEWEB)

    Prusakov, V.N. [Kurchatov Institute, Moscow (Russian Federation)

    1994-12-31

    The principles and process of centrifuge isotope separation are reviewed; the fundamental advantage of the centrifuge over gaseous diffusion arises from the fact that the primary isotope separation effect occurs at thermodynamic equilibrium; thus, gas centrifuge enrichment uses only about 1/20 to 1/30 of the electricity per SWU (separation work unit) consumed by gaseous diffusion. The various substances that can be used in centrifuge isotope separation are listed (fluorides, oxyfluorides, {pi}-complexes, boron hydrides, metal-organic compounds, halides...). The centrifuge method productivity is much greater than with the electromagnetic separation technique; examples of centrifuge stable and radioactive isotope separation are given; the method of the residue reduction is also presented with the example of separating radioactive krypton-85 out of a nuclear reactor krypton blend. 4 figs.

  11. Stable isotopic characterisation of francolite formation

    Science.gov (United States)

    McArthur, J. M.; Benmore, R. A.; Coleman, M. L.; Soldi, C.; Yeh, H.-W.; O'Brien, G. W.

    1986-02-01

    Stable isotopic data are presented for 112 samples of francolite from 18 separate phosphate deposits. Values of δ 13C and δ 34S in most offshore deposits suggest formation within oxic or suboxic environments either by carbonate replacement or direct precipitation of francolite from water of normal marine compositions. The exceptions are concretionary francolite from Namibia, which has an isotopic composition in keeping with its formation within organic-rich sediments, and that from offshore Morocco, which has an isotopic signature of the anoxic/suboxic interface. Onshore deposits from Jordan, Mexico, South Africa and, possibly, the Permian Phosphoria Formation in the western U.S.A., are substantially depleted in 18O: they appear to be too altered for deductions to be made about their environments of formation. In other onshore deposits which are unaltered, or minimally altered, the isotopic composition suggests that some formed within sulphate-reducing sediments (Sedhura, Morocco) whilst francolite from the Georgina Basin of Australia formed at the oxic/anoxic boundary, where oxidation of biogenic H 2S decreases the δ 34S of pore water. In general, pelletal samples show non-oxic isotopic signatures, whilst non-pelletal samples show oxic isotopic signatures, but samples from Namibia, Peru (Ica Plateau) and the Californian and Moroccan margins are exceptions to this rule. Morphology may therefore be a misleading indicator of francolite genesis as no definitive relation exists between phosphorite type and isotopic signature.

  12. Stable isotope enrichment using a plasma centrifuge

    Science.gov (United States)

    Krishnan, Mahadevan; Bures, Brian; Madden, Robert

    2012-10-01

    A primary goal of the Department of Energy's Isotope Development and Production for Research and Applications Program (Isotope Program) within the Office of Nuclear Physics (NP) is to produce isotopes that are in short supply in the U.S. and of which there exists no or insufficient domestic commercial production capability. A vacuum arc plasma centrifuge is a rigid rotor column of metal plasma in which centrifugal forces re-distribute ions radially according to their mass/charge ratio. Early work demonstrated rotation at 2 million rpm and separation of various stable isotopes. The spinning plasma column had a Gaussian flux profile, peaked on the rigid rotor axis. This work adopts a more efficient approach, with the plasma created as a hollow column, wherein the flux is concentrated at larger radii where the centrifugal action is highest. By tailoring the vacuum arc discharge geometry, the rotation rate can also be increased to ˜10 million rpm. Data from Cu, Al and other metal plasmas will be presented and discussed in light of enriched stable isotopes needed for research and medicine.

  13. Quantifying uncertainty in stable isotope mixing models

    Science.gov (United States)

    Davis, Paul; Syme, James; Heikoop, Jeffrey; Fessenden-Rahn, Julianna; Perkins, George; Newman, Brent; Chrystal, Abbey E.; Hagerty, Shannon B.

    2015-05-01

    Mixing models are powerful tools for identifying biogeochemical sources and determining mixing fractions in a sample. However, identification of actual source contributors is often not simple, and source compositions typically vary or even overlap, significantly increasing model uncertainty in calculated mixing fractions. This study compares three probabilistic methods, Stable Isotope Analysis in R (SIAR), a pure Monte Carlo technique (PMC), and Stable Isotope Reference Source (SIRS) mixing model, a new technique that estimates mixing in systems with more than three sources and/or uncertain source compositions. In this paper, we use nitrate stable isotope examples (δ15N and δ18O) but all methods tested are applicable to other tracers. In Phase I of a three-phase blind test, we compared methods for a set of six-source nitrate problems. PMC was unable to find solutions for two of the target water samples. The Bayesian method, SIAR, experienced anchoring problems, and SIRS calculated mixing fractions that most closely approximated the known mixing fractions. For that reason, SIRS was the only approach used in the next phase of testing. In Phase II, the problem was broadened where any subset of the six sources could be a possible solution to the mixing problem. Results showed a high rate of Type I errors where solutions included sources that were not contributing to the sample. In Phase III some sources were eliminated based on assumed site knowledge and assumed nitrate concentrations, substantially reduced mixing fraction uncertainties and lowered the Type I error rate. These results demonstrate that valuable insights into stable isotope mixing problems result from probabilistic mixing model approaches like SIRS. The results also emphasize the importance of identifying a minimal set of potential sources and quantifying uncertainties in source isotopic composition as well as demonstrating the value of additional information in reducing the uncertainty in calculated

  14. Sedimentary records of mercury stable isotopes in Lake Michigan

    Directory of Open Access Journals (Sweden)

    Runsheng Yin

    2016-01-01

    Full Text Available Abstract Mercury (Hg concentrations and Hg isotopic composition were investigated in three sediment cores in Lake Michigan (LM. Two cores were collected from Green Bay, a region heavily impacted by Hg contamination and one core from an offshore region of LM absent of direct point source Hg. Historical trends of Hg influxes suggest increased Hg deposition began in the 1890s in Green Bay and in the early 1800’s in offshore LM. Recently deposited sediment reflecting more anthropogenic influence shows similar δ202 Hg values (-1.0 to -0.5‰ for all three cores however, deep core sediments, reflecting pre-industrial eras, show much lower δ202Hg values (-1.7 to -1.2‰. Using a binary mixing model based on δ202Hg signatures, the proportion of anthropogenic Hg was estimated. Model output confirms that Green Bay is more contaminated by local point source than the offshore LM. An increase in positive Δ199Hg values (-0.02 to +0.27‰ was observed from inner Green Bay to the offshore of LM, which may indicate increased input of atmospheric Hg and decreased watershed inputs along this transect. Overall, this study suggests that sedimentary Hg isotopes maybe a useful tracer in understanding Hg sources and history of Hg contamination in large lakes.

  15. Using mercury isotopes to understand the bioaccumulation of Hg in the subtropical Pearl River Estuary, South China.

    Science.gov (United States)

    Yin, Runsheng; Feng, Xinbin; Zhang, Junjun; Pan, Ke; Wang, Wenxiong; Li, Xiangdong

    2016-03-01

    Coastal and estuarine regions are important areas of mercury pollution. Therefore, it is important to properly characterize the sources and bioaccumulation processes of mercury in these regions. Here, we present mercury stable isotopic compositions in 18 species of wild marine fish collected from the Pearl River Estuary (PRE), south China. Our results showed variations in mass-independent fractionation (Δ(199)Hg: +0.05 ± 0.10‰ to +0.59 ± 0.30‰) with a Δ(199)Hg/Δ(201)Hg of ∼1.26, suggesting that aqueous MeHg underwent photo-degradation prior to incorporation into the food chain. For the results, we discovered small but significant differences of Δ(199)Hg values among herbivorous, demersal, and carnivorous fish, indicating that different feeding guilds of fish may have incorporated MeHg with various degrees of photo-demethylation. The consistent mercury isotope compositions between fish feeding habitat and mercury sources in the estuary provide potentially important findings on the transformation and bioaccumulation of this toxic metal in subtropical coastal environments.

  16. Stable isotopic analyses in paleoclimatic reconstruction

    Energy Technology Data Exchange (ETDEWEB)

    Wigand, P.E. [Univ. and Community College System of Nevada, Reno, NV (United States)

    1995-09-01

    Most traditional paleoclimatic proxy data have inherent time lags between climatic input and system response that constrain their use in accurate reconstruction of paleoclimate chronology, scaling of its variability, and the elucidation of the processes that determine its impact on the biotic and abiotic environment. With the exception of dendroclimatology, and studies of short-lived organisms and pollen recovered from annually varved lacustrine sediments, significant periods of time ranging from years, to centuries, to millennia may intervene between climate change and its first manifestation in paleoclimatic proxy data records. Reconstruction of past climate through changes in plant community composition derived from pollen sequences and plant remains from ancient woodrat middens, wet environments and dry caves all suffer from these lags. However, stable isotopic analyses can provide more immediate indication of biotic response to climate change. Evidence of past physiological response of organisms to changes in effective precipitation as climate varies can be provided by analyses of the stable isotopic content of plant macrofossils from various contexts. These analyses consider variation in the stable isotopic (hydrogen, oxygen and carbon) content of plant tissues as it reflects (1) past global or local temperature through changes in meteoric (rainfall) water chemistry in the case of the first two isotopes, and (2) plant stress through changes in plant respiration/transpiration processes under differing water availability, and varying atmospheric CO, composition (which itself may actually be a net result of biotic response to climate change). Studies currently being conducted in the Intermountain West indicate both long- and short-term responses that when calibrated with modem analogue studies have the potential of revealing not only the timing of climate events, but their direction, magnitude and rapidity.

  17. Mercury contamination and stable isotopes reveal variability in foraging ecology of generalist California gulls

    Science.gov (United States)

    Peterson, Sarah; Ackerman, Josh; Eagles-Smith, Collin A.

    2017-01-01

    Environmental contaminants are a concern for animal health, but contaminant exposure can also be used as a tracer of foraging ecology. In particular, mercury (Hg) concentrations are highly variable among aquatic and terrestrial food webs as a result of habitat- and site-specific biogeochemical processes that produce the bioaccumulative form, methylmercury (MeHg). We used stable isotopes and total Hg (THg) concentrations of a generalist consumer, the California gull (Larus californicus), to examine foraging ecology and illustrate the utility of using Hg contamination as an ecological tracer under certain conditions. We identified four main foraging clusters of gulls during pre-breeding and breeding, using a traditional approach based on light stable isotopes. The foraging cluster with the highest δ15N and δ34S values in gulls (cluster 4) had mean blood THg concentrations 614% (pre-breeding) and 250% (breeding) higher than gulls with the lowest isotope values (cluster 1). Using a traditional approach of stable-isotope mixing models, we showed that breeding birds with a higher proportion of garbage in their diet (cluster 2: 63–82% garbage) corresponded to lower THg concentrations and lower δ15N and δ34S values. In contrast, gull clusters with higher THg concentrations, which were more enriched in 15N and 34S isotopes, consumed a higher proportion of more natural, estuarine prey. δ34S values, which change markedly across the terrestrial to marine habitat gradient, were positively correlated with blood THg concentrations in gulls. The linkage we observed between stable isotopes and THg concentrations suggests that Hg contamination can be used as an additional tool for understanding animal foraging across coastal habitat gradients.

  18. Nested reactor chamber and operation for Hg-196 isotope separation process

    Science.gov (United States)

    Grossman, M.W.

    1991-10-08

    The present invention is directed to an apparatus for use in [sup 196]Hg separation and its method of operation. Specifically, the present invention is directed to a nested reactor chamber useful for [sup 196]Hg isotope separation reactions avoiding the photon starved condition commonly encountered in coaxial reactor systems. 6 figures.

  19. Embryotoxicity of stable isotopes and use of stable isotopes in studies of teratogenetic mechanisms

    Energy Technology Data Exchange (ETDEWEB)

    Spielmann, H.; Nau, H.

    1986-07-01

    Experiments on teratogenic effects of stable isotopes from our own and other laboratories are evaluated. In the first series of investigations, the enrichment of the stable isotope /sup 13/C derived from U-/sup 13/C-glucose was studied in mouse embryos at various stages of development, including limb buds in organ culture. Preimplantation mouse embryos incubated in vitro in /sup 13/C-enriched medium for 48 hours showed normal development during subsequent differentiation in vitro and also in vivo after embryo transfer to faster mothers. These embryos were 15% to 20% enriched in /sup 13/C. Administration of U-13-C-glucose to pregnant mice during organogenesis led to an increase of the absolute /sup 13/C content of the embryo for several days after the end of isotope administration, whereas the enrichment in maternal tissue decreased. No alterations of embryonic development were detected due to stable isotope enrichment. Development of cultured mouse limb buds was unaffected by incubation with 82 mol% U-/sup 13/C-glucose as judged from morphologic and biochemical criteria. The second part of the article describes the value of deuterium-labeled drugs as probes into the mechanism of activation of teratogenic metabolites. A comparison of the pharmacokinetics as well as the teratogenicity between cyclophosphamide and some specific deuterium-labeled analogues showed that the isotope effect observed can be related to a particular metabolic pathway crucial for teratogenic activation by this drug.

  20. Stable isotope production with laser techniques; Production d`isotopes stables a l`aide des techniques laser

    Energy Technology Data Exchange (ETDEWEB)

    Petit, A. [CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France). Dept. des Procedes d`Enrichissement

    1994-12-31

    Laser properties may be used for stable isotope production either by selective photoionization of an atom particular isotope, either by selective photodissociation of a molecule. Principles of both processes are reviewed and examples of calcium 43 isotope separation through photoionization and of carbon and oxygen isotope separation by photodissociation are presented. 4 figs., 1 tab., 11 refs.

  1. Applications of stable isotope analysis in mammalian ecology.

    Science.gov (United States)

    Walter, W David; Kurle, Carolyn M; Hopkins, John B

    2014-01-01

    In this editorial, we provide a brief introduction and summarize the 10 research articles included in this Special Issue on Applications of stable isotope analysis in mammalian ecology. The first three articles report correction and discrimination factors that can be used to more accurately estimate the diets of extinct and extant mammals using stable isotope analysis. The remaining seven applied research articles use stable isotope analysis to address a variety of wildlife conservation and management questions from the oceans to the mountains.

  2. Utilization of stable isotopes in power reactor; Utilisation des isotopes stables dans les reacteurs de puissance

    Energy Technology Data Exchange (ETDEWEB)

    Desmoulins, P. [Electricite de France (EDF), 75 - Paris (France)

    1994-12-31

    The stable isotopes, besides uranium, used in EDF power nuclear reactors are mainly the boron 10 and the lithium 7. Boron is used in reactors as a neutrophagous agent for core reactivity control, and lithium, and more especially lithium 7, is extensively used as a solution in PWR moderators for primary fluid pH control. Boron and lithium ore reserves and producers are presented; industrial isotopic separation techniques are described: for the boron 10, they include dissociative distillation (Sulzer process) and separation on anionic resins, and for lithium 7, ion exchange columns (Cogema). 1 tab.

  3. Identifying the sources and processes of mercury in subtropical estuarine and ocean sediments using Hg isotopic composition.

    Science.gov (United States)

    Yin, Runsheng; Feng, Xinbin; Chen, Baowei; Zhang, Junjun; Wang, Wenxiong; Li, Xiangdong

    2015-02-03

    The concentrations and isotopic compositions of mercury (Hg) in surface sediments of the Pearl River Estuary (PRE) and the South China Sea (SCS) were analyzed. The data revealed significant differences between the total Hg (THg) in fine-grained sediments collected from the PRE (8-251 μg kg(-1)) and those collected from the SCS (12-83 μg kg(-1)). Large spatial variations in Hg isotopic compositions were observed in the SCS (δ(202)Hg, from -2.82 to -2.10‰; Δ(199)Hg, from +0.21 to +0.45‰) and PRE (δ(202)Hg, from -2.80 to -0.68‰; Δ(199)Hg, from -0.15 to +0.16‰). The large positive Δ(199)Hg in the SCS indicated that a fraction of Hg has undergone Hg(2+) photoreduction processes prior to incorporation into the sediments. The relatively negative Δ(199)Hg values in the PRE indicated that photoreduction of Hg is not the primary route for the removal of Hg from the water column. The riverine input of fine particles played an important role in transporting Hg to the PRE sediments. In the deep ocean bed of the SCS, source-related signatures of Hg isotopes may have been altered by natural geochemical processes (e.g., Hg(2+) photoreduction and preferential adsorption processes). Using Hg isotope compositions, we estimate that river deliveries of Hg from industrial and urban sources and natural soils could be the main inputs of Hg to the PRE. However, the use of Hg isotopes as tracers in source attribution could be limited because of the isotope fractionation by natural processes in the SCS.

  4. STABLE ISOTOPE GEOCHEMISTRY OF MASSIVE ICE

    Directory of Open Access Journals (Sweden)

    Yurij K. Vasil’chuk

    2016-01-01

    Full Text Available The paper summarises stable-isotope research on massive ice in the Russian and North American Arctic, and includes the latest understanding of massive-ice formation. A new classification of massive-ice complexes is proposed, encompassing the range and variabilityof massive ice. It distinguishes two new categories of massive-ice complexes: homogeneousmassive-ice complexes have a similar structure, properties and genesis throughout, whereasheterogeneous massive-ice complexes vary spatially (in their structure and properties andgenetically within a locality and consist of two or more homogeneous massive-ice bodies.Analysis of pollen and spores in massive ice from Subarctic regions and from ice and snow cover of Arctic ice caps assists with interpretation of the origin of massive ice. Radiocarbon ages of massive ice and host sediments are considered together with isotope values of heavy oxygen and deuterium from massive ice plotted at a uniform scale in order to assist interpretation and correlation of the ice.

  5. Mother-embryo isotope (δ¹⁵N, δ¹³C) fractionation and mercury (Hg) transfer in aplacental deep-sea sharks.

    Science.gov (United States)

    Le Bourg, B; Kiszka, J; Bustamante, P

    2014-05-01

    Stable carbon (δ¹³C) and nitrogen (δ¹⁵N) isotopic values and total mercury (Hg) concentrations were analysed in muscle and liver of mothers and embryos of two aplacental shark species, Squalus megalops and Centrophorus moluccensis. Embryos of the two species had similar or lower isotopic values than their respective mothers, the only exception being for δ¹³C, which was higher in the liver of C. moluccensis embryos than in their mothers. Hg concentrations were systematically lower in embryos compared with their mothers suggesting a low transfer of this element in muscle and liver.

  6. Mass dependent stable isotope fractionation of mercury during mer mediated microbial degradation of monomethylmercury

    Science.gov (United States)

    Kritee, K.; Barkay, Tamar; Blum, Joel D.

    2009-03-01

    Controlling bioaccumulation of toxic monomethylmercury (MMHg) in aquatic food chains requires differentiation between biotic and abiotic pathways that lead to its production and degradation. Recent mercury (Hg) stable isotope measurements of natural samples suggest that Hg isotope ratios can be a powerful proxy for tracing dominant Hg transforming pathways in aquatic ecosystems. Specifically, it has been shown that photo-degradation of MMHg causes both mass dependent (MDF) and mass independent fractionation (MIF) of Hg isotopes. Because the extent of MDF and MIF observed in natural samples (e.g., fish, soil and sediments) can potentially be used to determine the relative importance of pathways leading to MMHg accumulation, it is important to determine the potential role of microbial pathways in contributing to the fractionation, especially MIF, observed in these samples. This study reports the extent of fractionation of Hg stable isotopes during degradation of MMHg to volatile elemental Hg and methane via the microbial Hg resistance ( mer) pathway in Escherichia coli carrying a mercury resistance ( mer) genetic system on a multi-copy plasmid. During experimental microbial degradation of MMHg, MMHg remaining in reactors became progressively heavier (increasing δ202Hg) with time and underwent mass dependent Rayleigh fractionation with a fractionation factor α202/198 = 1.0004 ± 0.0002 (2SD). However, MIF was not observed in any of the microbial MMHg degradation experiments indicating that the isotopic signature left by mer mediated MMHg degradation is significantly different from fractionation observed during DOC mediated photo-degradation of MMHg. Additionally, a clear suppression of Hg isotope fractionation, both during reduction of Hg(II) and degradation of MMHg, was observed when the cell densities increased, possibly due to a reduction in substrate bioavailability. We propose a multi-step framework for understanding the extent of fractionation seen in our MMHg

  7. Mercury (Hg) in meteorites: variations in abundance, thermal release profile, mass-dependent and mass-independent isotopic fractionation

    CERN Document Server

    Meier, Matthias M M; Marty, Bernard

    2016-01-01

    We have measured the concentration, isotopic composition and thermal release profiles of Mercury (Hg) in a suite of meteorites, including both chondrites and achondrites. We find large variations in Hg concentration between different meteorites (ca. 10 ppb to 14'000 ppb), with the highest concentration orders of magnitude above the expected bulk solar system silicates value. From the presence of several different Hg carrier phases in thermal release profiles (150 to 650 {\\deg}C), we argue that these variations are unlikely to be mainly due to terrestrial contamination. The Hg abundance of meteorites shows no correlation with petrographic type, or mass-dependent fractionation of Hg isotopes. Most carbonaceous chondrites show mass-independent enrichments in the odd-numbered isotopes 199Hg and 201Hg. We show that the enrichments are not nucleosynthetic, as we do not find corresponding nucleosynthetic deficits of 196Hg. Instead, they can partially be explained by Hg evaporation and redeposition during heating of ...

  8. Stable isotope customer list and summary of shipments - FY 1983

    Energy Technology Data Exchange (ETDEWEB)

    Davis, W.C. (comp.)

    1983-12-01

    This compilation is published as an aid to those concerned with the separation and sale of stable isotopes. The information is divided into four sections: (1) alphabetical list of domestic and foreign customers, showing the stable isotopes purchased during the fiscal year; (2) alphabetical list of isotopes, cross-referenced to customer numbers and divided into domestic and foreign categories; (3) alphabetical list of states and countries, cross-referenced to customer numbers and indicating geographical concentrations of isotope users; and (4) tabulation of the shipments, quantities, and dollars for domestic, foreign, and project categories for each isotope.

  9. Stable Isotope Customer List and Summary of Shipments. FY 1985

    Energy Technology Data Exchange (ETDEWEB)

    Tracy, J. G. [comp.

    1985-11-01

    This compilation is published as an aid to those concerned with the separation and sale of stable isotopes. The information is divided into four sections: (1) alphabetical list of domestic and foreign customers, showing the stable isotopes purchased during the fiscal year; (2) alphabetical list of isotopes, cross-referenced to customer numbers and divided into domestic and foreign categories; (3) alphabetical list of states and countries, cross-referenced to customer numbers and indicating geographical concentrations of isotope users; and (4) tabulation of the shipments, quantities, and dollars for domestic, foreign, and project categories for each isotope.

  10. Stable isotope methods in biological and ecological studies of arthropods

    NARCIS (Netherlands)

    Hood-Nowotny, R.C.; Knols, B.G.J.

    2007-01-01

    This is an eclectic review and analysis of contemporary and promising stable isotope methodologies to study the biology and ecology of arthropods. It is augmented with literature from other disciplines, indicative of the potential for knowledge transfer. It is demonstrated that stable isotopes can

  11. Degradation changes stable carbon isotope depth profiles in palsa peatlands

    Directory of Open Access Journals (Sweden)

    J. P. Krüger

    2014-01-01

    Full Text Available Palsa peatlands are a significant carbon pool in the global carbon cycle and are projected to change by global warming due to accelerated permafrost thaw. Our aim was to use stable carbon isotopes as indicators of palsa degradation. Depth profiles of stable carbon isotopes generally reflect organic matter dynamics in soils with an increase of δ13C values during aerobic decomposition and stable or decreasing δ13C values with depth during anaerobic decomposition. Stable carbon isotope depth profiles of undisturbed and degraded sites of hummocks as well as hollows at three palsa peatlands in northern Sweden were used to investigate the degradation processes. The depth patterns of stable isotopes clearly differ between intact and degraded hummocks at all sites. Erosion and cryoturbation at the degraded sites significantly changes the stable carbon isotope depth profiles. At the intact hummocks the uplifting of peat material by permafrost is indicated by a turning in the δ13C depth trend and this assessment is supported by a change in the C / N ratios. For hollows isotope patterns were less clear, but some hollows and degraded hollows in the palsa peatlands show differences in their stable carbon isotope depth profiles indicating enhanced degradation rates. We conclude that the degradation of palsa peatlands by accelerated permafrost thawing could be identified with stable carbon isotope depth profiles. At intact hummocks δ13C depth patterns display the uplifting of peat material by a change in peat decomposition processes.

  12. Trophic position of coexisting krill species: a stable isotope approach

    DEFF Research Database (Denmark)

    Agersted, Mette Dalgaard; Bode, Antonio; Nielsen, Torkel Gissel

    2014-01-01

    Four krill species with overlapping functional biology coexist in Greenland waters. Here, we used stable isotopes to investigate and discuss their trophic role and mode of coexistence. Bulk carbon (δ13C) and nitrogen (δ15N) stable isotope analyses of Thysanoessa longicaudata, T. inermis, T. rasch...

  13. Mass Dependent Fractionation of Hg Isotopes in Source Rocks, Mineral Deposits and Spring Waters of the California Coast Ranges, USA

    Science.gov (United States)

    Smith, C. N.; Kesler, S. E.; Blum, J. D.; Rytuba, J. J.

    2007-12-01

    We present here the first study of the isotopic composition of Hg in rocks, ore deposits, and active hydrothermal systems from the California Coast Ranges, one of Earth's largest Hg-depositing systems. The Franciscan Complex and Great Valley Sequence, which form the bedrock in the California Coast Ranges, are intruded and overlain by Tertiary volcanic rocks including the Clear Lake Volcanic Sequence. These rocks contain two types of Hg deposits, hot-spring deposits that form at shallow depths (geothermal systems that release Hg to the present surface. The Franciscan Complex and Great Valley Sequence contain clastic sedimentary rocks with higher concentrations of Hg than volcanic rocks of the Clear Lake Volcanic Field. Mean Hg isotope compositions for all three rock units are similar, although the range of values in Franciscan Complex rocks is greater than in either Great Valley or Clear Lake rocks. Hot spring and silica-carbonate Hg deposits have similar average isotopic compositions that are indistinguishable from averages for the three rock units, although δ202Hg values for the Hg deposits have a greater variance than the country rocks. Precipitates from dilute spring and saline thermal waters in the area have similarly large variance and a mean δ202Hg value that is significantly lower than the ore deposits and rocks. These observations indicate there is little or no isotopic fractionation during release of Hg from its source rocks into hydrothermal solutions. Isotopic fractionation does appear to take place during transport and concentration of Hg in deposits, especially in their uppermost parts. Boiling of hydrothermal fluids is likely the most important process causing of the observed Hg isotope fractionation. This should result in the release of Hg with low δ202Hg values into the atmosphere from the top of these hydrothermal systems and a consequent enrichment in heavy Hg isotopes in the upper crust through time.

  14. The separation of stable isotopes of carbon

    Science.gov (United States)

    Oziashvili, E. D.; Egiazarov, A. S.

    1989-04-01

    The present state of work on the separation of carbon isotopes by diffusion, fractional distillation, chemical isotopic exchange, and the selective excitation and dissociation of molecules in electrical discharges or in the field of laser radiation has been examined. The characteristics of new laboratory and industrial assemblies for separating carbon isotopes have been described. Promising directions of study aimed at developing effective technological processes for separating carbon isotopes have been noted. The bibliography contains 148 references.

  15. Stable isotope deltas: Tiny, yet robust signatures in nature

    Science.gov (United States)

    Brand, Willi A.; Coplen, Tyler B.

    2012-01-01

    Although most of them are relatively small, stable isotope deltas of naturally occurring substances are robust and enable workers in anthropology, atmospheric sciences, biology, chemistry, environmental sciences, food and drug authentication, forensic science, geochemistry, geology, oceanography, and paleoclimatology to study a variety of topics. Two fundamental processes explain the stable isotope deltas measured in most terrestrial systems: isotopic fractionation and isotope mixing. Isotopic fractionation is the result of equilibrium or kinetic physicochemical processes that fractionate isotopes because of small differences in physical or chemical properties of molecular species having different isotopes. It is shown that the mixing of radioactive and stable isotope end members can be modelled to provide information on many natural processes, including 14C abundances in the modern atmosphere and the stable hydrogen and oxygen isotopic compositions of the oceans during glacial and interglacial times. The calculation of mixing fractions using isotope balance equations with isotope deltas can be substantially in error when substances with high concentrations of heavy isotopes (e.g. 13C, 2H, and 18O ) are mixed. In such cases, calculations using mole fractions are preferred as they produce accurate mixing fractions. Isotope deltas are dimensionless quantities. In the International System of Units (SI), these quantities have the unit 1 and the usual list of prefixes is not applicable. To overcome traditional limitations with expressing orders of magnitude differences in isotope deltas, we propose the term urey (symbol Ur), after Harold C. Urey, for the unit 1. In such a manner, an isotope delta value expressed traditionally as−25 per mil can be written as−25 mUr (or−2.5 cUr or−0.25 dUr; the use of any SI prefix is possible). Likewise, very small isotopic differences often expressed in per meg ‘units’ are easily included (e.g. either+0.015 ‰ or+15 per meg

  16. Stable isotope deltas: tiny, yet robust signatures in nature.

    Science.gov (United States)

    Brand, Willi A; Coplen, Tyler B

    2012-09-01

    Although most of them are relatively small, stable isotope deltas of naturally occurring substances are robust and enable workers in anthropology, atmospheric sciences, biology, chemistry, environmental sciences, food and drug authentication, forensic science, geochemistry, geology, oceanography, and paleoclimatology to study a variety of topics. Two fundamental processes explain the stable isotope deltas measured in most terrestrial systems: isotopic fractionation and isotope mixing. Isotopic fractionation is the result of equilibrium or kinetic physicochemical processes that fractionate isotopes because of small differences in physical or chemical properties of molecular species having different isotopes. It is shown that the mixing of radioactive and stable isotope end members can be modelled to provide information on many natural processes, including (14)C abundances in the modern atmosphere and the stable hydrogen and oxygen isotopic compositions of the oceans during glacial and interglacial times. The calculation of mixing fractions using isotope balance equations with isotope deltas can be substantially in error when substances with high concentrations of heavy isotopes (e.g. (13)C, (2)H, and (18)O ) are mixed. In such cases, calculations using mole fractions are preferred as they produce accurate mixing fractions. Isotope deltas are dimensionless quantities. In the International System of Units (SI), these quantities have the unit 1 and the usual list of prefixes is not applicable. To overcome traditional limitations with expressing orders of magnitude differences in isotope deltas, we propose the term urey (symbol Ur), after Harold C. Urey, for the unit 1. In such a manner, an isotope delta value expressed traditionally as-25 per mil can be written as-25 mUr (or-2.5 cUr or-0.25 dUr; the use of any SI prefix is possible). Likewise, very small isotopic differences often expressed in per meg 'units' are easily included (e.g. either+0.015 ‰ or+15 per meg

  17. Stable isotope views on ecosystem function: challenging or challenged?

    Science.gov (United States)

    Resco, Víctor; Querejeta, José I.; Ogle, Kiona; Voltas, Jordi; Sebastià, Maria-Teresa; Serrano-Ortiz, Penélope; Linares, Juan C.; Moreno-Gutiérrez, Cristina; Herrero, Asier; Carreira, José A.; Torres-Cañabate, Patricia; Valladares, Fernando

    2010-01-01

    Stable isotopes and their potential for detecting various and complex ecosystem processes are attracting an increasing number of scientists. Progress is challenging, particularly under global change scenarios, but some established views have been challenged. The IX meeting of the Spanish Association of Terrestrial Ecology (AAET, Úbeda, 18–22 October 2009) hosted a symposium on the ecology of stable isotopes where the linear mixing model approach of partitioning sinks and sources of carbon and water fluxes within an ecosystem was challenged, and new applications of stable isotopes for the study of plant interactions were evaluated. Discussion was also centred on the need for networks that monitor ecological processes using stable isotopes and key ideas for fostering future research with isotopes. PMID:20015858

  18. Protein labelling with stable isotopes: strategies; Le marquage des proteines aux isotopes stables: strategies

    Energy Technology Data Exchange (ETDEWEB)

    Lirsac, P.N.; Gilles, N.; Jamin, N.; Toma, F.; Gabrielsen, O.; Boulain, J.C.; Menez, A. [CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France). Direction des Sciences du Vivant

    1994-12-31

    A protein labelling technique with stable isotopes has been developed at the CEA: a labelled complete medium has been developed, performing as well as the Luria medium, but differing from it because it contains not only free aminated acids and peptides, but also sugars (96% of D-glucopyrannose) and labelled nucleosides. These precursors are produced from a labelled photosynthetic micro-organisms biomass, obtained with micro-algae having incorporated carbon 13, nitrogen 15 and deuterium during their culture. Labelling costs are reduced. 1 fig., 1 tab., 3 refs.

  19. Mercury (Hg) in meteorites: Variations in abundance, thermal release profile, mass-dependent and mass-independent isotopic fractionation

    Science.gov (United States)

    Meier, Matthias M. M.; Cloquet, Christophe; Marty, Bernard

    2016-06-01

    We have measured the concentration, isotopic composition and thermal release profiles of Mercury (Hg) in a suite of meteorites, including both chondrites and achondrites. We find large variations in Hg concentration between different meteorites (ca. 10 ppb to 14,000 ppb), with the highest concentration orders of magnitude above the expected bulk solar system silicates value. From the presence of several different Hg carrier phases in thermal release profiles (150-650 °C), we argue that these variations are unlikely to be mainly due to terrestrial contamination. The Hg abundance of meteorites shows no correlation with petrographic type, or mass-dependent fractionation of Hg isotopes. Most carbonaceous chondrites show mass-independent enrichments in the odd-numbered isotopes 199Hg and 201Hg. We show that the enrichments are not nucleosynthetic, as we do not find corresponding nucleosynthetic deficits of 196Hg. Instead, they can partially be explained by Hg evaporation and redeposition during heating of asteroids from primordial radionuclides and late-stage impact heating. Non-carbonaceous chondrites, most achondrites and the Earth do not show these enrichments in vapor-phase Hg. All meteorites studied here have however isotopically light Hg (δ202Hg = ∼-7 to -1) relative to the Earth's average crustal values, which could suggest that the Earth has lost a significant fraction of its primordial Hg. However, the late accretion of carbonaceous chondritic material on the order of ∼2%, which has been suggested to account for the water, carbon, nitrogen and noble gas inventories of the Earth, can also contribute most or all of the Earth's current Hg budget. In this case, the isotopically heavy Hg of the Earth's crust would have to be the result of isotopic fractionation between surface and deep-Earth reservoirs.

  20. Environmental and biomedical applications of natural metal stable isotope variations

    Science.gov (United States)

    Bullen, T.D.; Walczyk, T.

    2009-01-01

    etal stable isotopes are now being used to trace metal contaminants in the environment and as indicators of human systemic function where metals play a role. Stable isotope abundance variations provide information about metal sources and the processes affecting metals in complex natural systems, complementing information gained from surrogate tracers, such as metal abundance ratios or biochemical markers of metal metabolism. The science is still in its infancy, but the results of initial studies confirm that metal stable isotopes can provide a powerful tool for forensic and biomedical investigations.

  1. Quantification of Labile Soil Mercury by Stable Isotope Dilution Techniques

    Science.gov (United States)

    Shetaya, Waleed; Huang, Jen-How; Osterwalder, Stefan; Alewell, Christine

    2016-04-01

    Mercury (Hg) is a toxic element that can cause severe health problems to humans. Mercury is emitted to the atmosphere from both natural and anthropogenic sources and can be transported over long distances before it is deposited to aquatic and terrestrial environments. Aside from accumulation in soil solid phases, Hg deposited in soils may migrate to surface- and ground-water or enter the food chain, depending on its lability. There are many operationally-defined extraction methods proposed to quantify soil labile metals. However, these methods are by definition prone to inaccuracies such as non-selectivity, underestimation or overestimation of the labile metal pool. The isotopic dilution technique (ID) is currently the most promising method for discrimination between labile and non-labile metal fractions in soil with a minimum disturbance to soil-solid phases. ID assesses the reactive metal pool in soil by defining the fraction of metal both in solid and solution phases that is isotopically-exchangeable known as the 'E-value'. The 'E-value' represents the metal fraction in a dynamic equilibrium with the solution phase and is potentially accessible to plants. This is carried out by addition of an enriched metal isotope to soil suspensions and quantifying the fraction of metal that is able to freely exchange with the added isotope by measuring the equilibrium isotopic ratio by ICP-MS. E-value (mg kg-1) is then calculated as follows: E-Value = (Msoil/ W) (CspikeVspike/ Mspike) (Iso1IAspike -Iso2IAspikeRss / Iso2IAsoil Rss - Iso1IAsoil) where M is the average atomic mass of the metal in the soil or the spike, W is the mass of soil (kg), Cspike is the concentration of the metal in the spike (mg L-1), Vspike is the volume of spike (L), IA is isotopic abundance, and Rss is the equilibrium ratio of isotopic abundances (Iso1:Iso2). Isotopic dilution has been successfully applied to determine E-values for several elements. However, to our knowledge, this method has not yet

  2. IUPAC Project: Terminology and definition of quantities related to the isotope distribution in elements with more than two stable isotopes

    Science.gov (United States)

    Kaiser, J.; Angert, A.; Bergquist, B.; Brand, W.; Ono, S.; Röckmann, T.; Savarino, J.

    2012-04-01

    The objective of IUPAC Project 2009-046-2-200 (http://www.iupac.org/web/ins/2009-046-2-200) is to define terminology and to identify the most suitable definitions of quantities that characterise the isotope distribution in elements with more than two stable isotopes, including so-called mass-independent fractionation, non-mass dependent fractionation, isotope anomaly, 17O excess, etc. Most atmospheric oxygen-bearing species show deviations in their triple oxygen isotope ratios from mass-dependent fractionation (MDF) relationships predicted by the theories of Urey, Bigeleisen and Mayer. Similar deviations have also been found in sulphur and other elements with more than two stables isotopes (e.g. Hg, Cd, Zn), often preserved in non-atmospheric reservoirs, including rocks, minerals, soils, ice and waters. Despite the ubiquity of this type of isotope anomaly, there has never been an attempt to clearly define the terminology and physical quantities used to measure these anomalies and the processes that lead to their formation. Terms like mass-independent fractionation, non-mass dependent fractionation, isotope anomaly, isotope excess etc. have been used in the historic and recent literature, but are often not carefully distinguished. The realisation that MDF comprises a range of possible relationships between the isotopes of one element led to further complications because it meant that apparent isotope anomalies could be created by a combination of different MDF processes. At the moment, at least four different definitions to quantify isotope anomalies are being used. Furthermore, coefficients used in these definitions vary, which makes the comparison of data from different sources very difficult, even for experts. A consistent set of recommendations on how to express and quantify the isotope distribution in elements with more than two stable isotopes is highly warranted. From our experience as academic teachers, we are woefully aware how impenetrable the field is for

  3. Fractionation of Metal Stable Isotopes by Higher Plants

    OpenAIRE

    Friedhelm von Blanckenburg; N. von Wirén; M. Guelke; Weiss, D J; T. D. Bullen

    2009-01-01

    Higher plants induce chemical reactions in the rhizosphere, facilitating metal uptake by roots. Fractionation of the isotopes in nutrients such as calcium, iron, magnesium, and zinc produces a stable isotope composition in the plants that generally differs from that of the growth medium. Isotope fractionation also occurs during transport of the metals within most plants, but its extent depends on plant species and on the metal, in particular, on the metal’s redox state and what ligand it is b...

  4. Assessment of stable isotope incorporation into recombinant proteins.

    Science.gov (United States)

    Zhang, Xin; Luo, Quanzhou; Apostol, Izydor; Luo, Shun; Jerums, Matthew; Huang, Gang; Jiang, Xinzhao Grace; Gastwirt, Jessica; Savjani, Nimesh; Lewis, Jeffrey; Keener, Ronald; Wypych, Jette

    2013-02-15

    Stable isotope labeling combined with mass spectrometry has been widely used in a diverse set of applications in the biochemistry and biomedical fields. When stable isotope-labeled proteins are produced via metabolic labeling of cell culture, a comprehensive assessment of the labeling pattern is imperative. In this study, we present a set of mass spectrometry-based bioanalytical tools developed for quantitatively tracing the levels of the stable isotopes incorporated into the recombinant proteins (monoclonal antibodies and Fc fusion proteins expressed in different host systems) that include total mass analysis, peptide mapping analysis, and amino acid analysis. We show that these three mass spectrometry-based analytical methods have distinctive advantages and limitations and that they are mutually complementary in evaluating the quality of stable isotope-labeled proteins. In addition, we show that the analytical techniques developed here are powerful tools to provide valuable insights into studying cell metabolism and performing flux analysis during cell culture.

  5. Arctic cisco stable isotope data, Prudhoe Bay, August 2009

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set documents the carbon and nitrogen stable isotope ratios of age-0 Arctic cisco (Coregonus autumnalis) captured in Prudhoe Bay, Alaska in August 2009....

  6. Stable isotopes: essential tools in biological and medical research

    Energy Technology Data Exchange (ETDEWEB)

    Klein, P. D.; Hachey, D. L.; Kreek, M. J.; Schoeller, D. A.

    1977-01-01

    Recent developments in the use of the stable isotopes, /sup 13/C, /sup 15/N, /sup 17/O, and /sup 18/O, as tracers in research studies in the fields of biology, medicine, pharmacology, and agriculture are briefly reviewed. (CH)

  7. BIODEGRADATION OF FLUORANTHENE AS MONITORED USING STABLE CARBON ISOTOPES

    Science.gov (United States)

    The measurement of stable isotope ratios of carbon (d13C values) was investigated as a viable technique to monitor the intrinsic bioremediation of polycyclic aromatic hydrocarbons (PAHs). Biometer-flask experiments were conducted in which the bacterium, Sphingomonas paucimobilis,...

  8. Stable carbon isotope ratios from archaeological charcoal as palaeoenvironmental indicators

    CSIR Research Space (South Africa)

    Hall, G

    2008-01-01

    Full Text Available The potential to provide environmental proxies using stable carbon isotopes from modern and archaeological charcoal is explored. Experiments on modern Podocarpus (Yellowwoods) show that δ13C values of stems, branches and charcoal preserve proxy...

  9. Stable isotope labeling strategy based on coding theory

    Energy Technology Data Exchange (ETDEWEB)

    Kasai, Takuma; Koshiba, Seizo; Yokoyama, Jun; Kigawa, Takanori, E-mail: kigawa@riken.jp [RIKEN Quantitative Biology Center (QBiC), Laboratory for Biomolecular Structure and Dynamics (Japan)

    2015-10-15

    We describe a strategy for stable isotope-aided protein nuclear magnetic resonance (NMR) analysis, called stable isotope encoding. The basic idea of this strategy is that amino-acid selective labeling can be considered as “encoding and decoding” processes, in which the information of amino acid type is encoded by the stable isotope labeling ratio of the corresponding residue and it is decoded by analyzing NMR spectra. According to the idea, the strategy can diminish the required number of labelled samples by increasing information content per sample, enabling discrimination of 19 kinds of non-proline amino acids with only three labeled samples. The idea also enables this strategy to combine with information technologies, such as error detection by check digit, to improve the robustness of analyses with low quality data. Stable isotope encoding will facilitate NMR analyses of proteins under non-ideal conditions, such as those in large complex systems, with low-solubility, and in living cells.

  10. Beam delivery for stable isotope separation

    Science.gov (United States)

    Forbes, Andrew; Strydom, Hendrick J.; Botha, Lourens R.; Ronander, Einar

    2002-10-01

    In the multi-photon dissociation process of Carbon isotope enrichment, IR photons are used to selectively excite a molecule with the given isotopic base element. This enrichment process is very sensitive to the beam's intensity and wavelength. Because the intensity is determined by the propagation of the field, the enrichment factors are also very dependent on the field propagation. In this paper, the influence of the wavelength and intensity of the beam, on the isotope selective dissociation of a CFC compound is investigated both experimentally and theoretically. Consideration is also given to some of the factors that influence the delivery of various beams to the reactor chamber, and their subsequent propagation through the reactor. The results show that suitable beam forming can lead to an improved isotope separation process.

  11. Stable Oxygen-18 and Deuterium Isotopes

    DEFF Research Database (Denmark)

    Müller, Sascha

    development within a given lowland headwater catchment (stream water isotopes). Based on our investigations on the precipitation isotopic composition a local meteoric water line (LMWL) was constructed and expressed as: δ2H=7.4 δ18O + 5.36‰. Moreover, we showed that under maritime temperature climate influence......, humidity is the main driver for the temporal and spatial isotopic development and temperature plays only a secondary role. Based on our investigations at a coastal lagoon aquifer we could improve quantifications of seasonal saltwater-wedge dynamics, based on isotopic tracers combined with salinity. We can...... link the observed seasonal regression/transgression pattern to the inland recharge cycle, which is expressed in hydraulic head configuration and submarine groundwater discharge rates at the fieldsite. Nonetheless, those observed dynamics could not be simulated due to numerical limitations. By the use...

  12. ESTIMATING THE TIMING OF DIET SHIFTS USING STABLE ISOTOPES

    Science.gov (United States)

    Stable isotope analysis has become an important tool in studies of trophic food webs and animal feeding patterns. When animals undergo rapid dietary shifts due to migration, metamorphosis, or other reasons, the isotopic composition of their tissues begins changing to reflect tha...

  13. Carbon Stable Isotopes as Indicators of Coastal Eutrophication

    Science.gov (United States)

    Coastal ecologists and managers have frequently used nitrogen stable isotopes (δ15N) to trace and monitor anthropogenic nitrogen (N) in coastal ecosystems. However, the interpretation of δ15N data can often be challenging, if not confounding, as the isotope values fractionate su...

  14. USE OF STABLE ISOTOPES IN ENVIRONMENTAL AND FORENSIC GEOCHEMISTRY STUDIES

    Science.gov (United States)

    Stable carbon and hydrogen isotopes have been used for many decades in the petroleum industry, but the development of combined gas chromatography-isotope ratio mass spectrometry (GCIRMS) has led to a virtual explosion in application of this technique not only in petroleum explora...

  15. Stable isotopes in pharmacology studies: present and future

    Energy Technology Data Exchange (ETDEWEB)

    Browne, T.R.

    1986-07-01

    Stable-isotope techniques offer advantages over older methods in safety, sensitivity, specificity, and reduction in numbers of subjects required and analytic determinations for some types of pharmacology studies. In addition to their use as internal standards in gas chromatography-mass spectrometry analytic methods, stable isotopes have been successfully employed in studies of absorption, bioavailability, distribution, biotransformation, excretion, metabolite identification, time-dependent and dose-dependent pharmacokinetic changes, drug interactions, pharmacologic changes during pregnancy, mutagenicity, and teratogenicity. 32 references.

  16. Stable Chlorine Isotope Study: Application to Early Solar System Materials

    Science.gov (United States)

    Mala,ira. M/; Nyquist, L. E.; Reese, Y.; Shih, C-Y; Fujitani, T.; Okano, O.

    2010-01-01

    A significantly large mass fractionation between two stable chlorine isotopes is expected during planetary processes In addition, in view of the isotopic heterogeneity of other light elements, the chlorine isotopes can potentially be used as a tracer for the origins and evolutionary processes of early solar system materials. Due to analytical difficulties, however, current chlorine isotope studies on planetary materials are quite controversial among IRMS (gas source mass spectrometry) and/or TIMS (Thermal Ionization Mass Spectrometry) groups [i.e. 1-3]. Although a cross-calibration of IRMS and TIMS indicates that both techniques are sufficiently consistent with each other [4], some authors have claimed that the Cl-37/Cl-35 ratio of geological samples obtained by TIMS technique are, in general, misleadingly too high and variable compared to those of IRMS [3]. For example, almost no differences of Cl isotope composition were observed among mantle materials and carbonaceous meteorites by [3]. On the other hand, according to more recent IRMS work [2], significant Cl isotope variations are confirmed for mantle materials. Therefore, additional careful investigation of Cl isotope analyses are now required to confirm real chlorine isotope variations for planetary materials including carbonaceous chondrites [5]. A significantly large mass fractionation between two stable chlorine isotopes is expected during planetary processes In addition, in view of the isotopic heterogeneity of other light elements, the chlorine isotopes can potentially be used as a tracer for the origins and evolutionary processes of early solar system materials. Due to analytical difficulties, however, current chlorine isotope studies on planetary materials are quite controversial among IRMS (gas source mass spectrometry) and/or TIMS (Thermal Ionization Mass Spectrometry) groups [i.e. 1-3]. Although a cross-calibration of IRMS and TIMS indicates that both techniques are sufficiently consistent with each

  17. Tracing food webs with stable hydrogen isotopes.

    Science.gov (United States)

    Estep, M F; Dabrowski, H

    1980-09-26

    The hydrogen isotopic content of an animal's food, not water, determines that animal's hydrogen isotopic content. Liver and muscle tissue from mice reared on a diet such that the ratio of deuterium to hydrogen (DIH) of their food and water was kept constant, have the same average D/H ratio as the food source. In a simple, natural population of snails and their possible algal diets, Littorina obtusata (northern Atlantic intertidal snails that feed almost exclusively on the brown alga Fucus vesiculosus) has the same D/H ratio as Fucus vesiculosis and not that of the other algae available to the snails.

  18. Stable isotope ecology in the Omo-Turkana Basin.

    Science.gov (United States)

    Cerling, Thure E; Levin, Naomi E; Passey, Benjamin H

    2011-01-01

    Stable isotopes provide an independent assessment of paleoenvironments in the Omo-Turkana Basin. Stable isotopes track the flow of oxygen and carbon through ecosystems and accordingly are not directly related to changes in mammalian faunal composition or sedimentology. Therefore, isotope studies give insight into the paleoenvironmental conditions in which human evolutionary trends have been recorded. The development of stable isotopes as indicators of continental environmental conditions has proceeded in parallel with questions about the conditions of human environment. What was the vegetation? How hot was it? How dry? What were the diets of animals living among early humans? And most persistently, how important were "savannas" to early hominids? In this review, we take the opportunity to provide extensive background on the use of isotopes in anthropological sites. The application of stable isotope ecology to anthropological sites in the Turkana Basin has a long history, but in many ways the Omo-Turkana Basin has been a proving ground for the development of new proxy methods for understanding tropical terrestrial environments in the Neogene and Quaternary. For that reason, we also describe some of the fundamental aspects of isotope ecology that developed outside the field of paleoanthropology. Copyright © 2011 Wiley Periodicals, Inc.

  19. Development of O-18 stable isotope separation technology using membrane

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jae Woo; Kim, Taek Soo; Choi, Hwa Rim; Park, Sung Hee; Lee, Ki Tae; Chang, Dae Shik

    2006-06-15

    The ultimate goal of this investigation is to develop the separation technology for O-18 oxygen stable isotope used in a cyclotron as a target for production of radioisotope F-18. F-18 is a base material for synthesis of [F-18]FDG radio-pharmaceutical, which is one of the most important tumor diagnostic agent used in PET (Positron Emission Tomography). More specifically, this investigation is focused on three categories as follow, 1) development of the membrane distillation isotope separation process to re-enrich O-18 stable isotope whose isotopic concentration is reduced after used in a cyclotron, 2) development of organic impurity purification technology to remove acetone, methanol, ethanol, and acetonitrile contained in a used cyclotron O-18 enriched target water, and 3) development of a laser absorption spectroscopic system for analyzing oxygen isotopic concentration in water.

  20. Fractionation of metal stable isotopes by higher plants

    Science.gov (United States)

    Von Blanckenburg, F.; Von Wiren, N.; Guelke, M.; Weiss, D.J.; Bullen, T.D.

    2009-01-01

    Higher plants induce chemical reactions in the rhizosphere, facilitating metal uptake by roots. Fractionation of the isotopes in nutrients such as calcium, iron, magnesium, and zinc produces a stable isotope composition in the plants that generally differs from that of the growth medium. Isotope fractionation also occurs during transport of the metals within most plants, but its extent depends on plant species and on the metal, in particular, on the metal's redox state and what ligand it is bound to. The metal stable isotope variations observed in plants create an isotope signature of life at the Earth's surface, contributing substantially to our understanding of metal cycling processes in the environment and in individual organisms.

  1. Identification of contamination in a lake sediment core using Hg and Pb isotopic compositions, Lake Ballinger, Washington, USA

    Science.gov (United States)

    Gray, John E.; Pribil, Michael J.; Van Metre, Peter C.; Borrok, David M.; Thapalia, Anita

    2013-01-01

    Concentrations and isotopic compositions of Hg and Pb were measured in a sediment core collected from Lake Ballinger, near Seattle, Washington, USA. Lake Ballinger has been affected by input of metal contaminants emitted from the Tacoma smelter, which operated from 1887 to 1986 and was located about 53 km south of the lake. Concentrations and loadings of Hg and Pb in Lake Ballinger increased by as much as three orders of magnitude during the period of smelting as compared to the pre-smelting period. Concentrations and loadings of Hg and Pb then decreased by about 55% and 75%, respectively, after smelting ended. Isotopic compositions of Hg changed considerably during the period of smelting (δ202Hg = −2.29‰ to −0.38‰, mean −1.23‰, n = 9) compared to the pre-smelting period (δ202Hg = −2.91‰ to −2.50‰, mean −2.75‰, n = 4). Variations were also observed in 206Pb/207Pb and 208Pb/207Pb isotopic compositions during these periods. Data for Δ199Hg and Δ201Hg indicate mass independent fractionation (MIF) of Hg isotopes in Lake Ballinger sediment during the smelting and post-smelting period and suggest MIF in the ore smelted, during the smelting process, or chemical modification at some point in the past. Negative values for Δ199Hg and Δ201Hg for the pre-smelting period are similar to those previously reported for soil, peat, and lichen, likely suggesting some component of atmospheric Hg. Variations in the concentrations and isotopic compositions of Hg and Pb were useful in tracing contaminant sources and the understanding of the depositional history of sedimentation in Lake Ballinger.

  2. Application of enriched stable isotopes as tracers in biological systems

    DEFF Research Database (Denmark)

    Stürup, Stefan; Hansen, Helle Rüsz; Gammelgaard, Bente

    2008-01-01

    The application of enriched stable isotopes of minerals and trace elements as tracers in biological systems is a rapidly growing research field that benefits from the many new developments in inorganic mass spectrometric instrumentation, primarily within inductively coupled plasma mass spectrometry...... (ICP-MS) instrumentation, such as reaction/collision cell ICP-MS and multicollector ICP-MS with improved isotope ratio measurement and interference removal capabilities. Adaptation and refinement of radioisotope tracer experiment methodologies for enriched stable isotope experiments......, and the development of new methodologies coupled with more advanced compartmental and mathematical models for the distribution of elements in living organisms has enabled a broader use of enriched stable isotope experiments in the biological sciences. This review discusses the current and future uses of enriched...

  3. Stable Isotopes and Oral Tori in Greenlandic Norse and Inuit

    DEFF Research Database (Denmark)

    Baumann, M.; Lynnerup, N.; Scott, G. R.

    2017-01-01

    that estimated stable carbon and nitrogen isotope compositions for a Greenlandic Norse sample makes it possible to compare directly PT and MT expression with the relative degree of marine protein intake. For comparative purposes, parallel observations were made on a Greenlandic Inuit sample. Some researchers...... suggest the intake of marine resources could impact bone development, including torus expression, but our analysis found no significant correlation between PT or MT expression and δ13C and δ15N values in the Norse. In the Inuit, PT expression also showed no relationship to stable isotope compositions. MT...... size in the Inuit did, however, show a significant inverse relationship with δ13C and δ15N values. As MT size goes up, stable isotope compositions go down. Compared with contemporary European populations, the Greenlandic Norse show very positive isotope compositions, but the Inuit, with their high...

  4. Comparison of Self-Consistent Skyrme and Gogny Calculations for Light Hg Isotopes

    CERN Document Server

    Warda, M; Próchniak, L

    2009-01-01

    The ground-state properties of neutron-deficient Hg isotopes have been investigated by the constrained self-consistent Hartree-Fock-Bogoliubov approach with the Skyrme and Gogny effective forces. In the case of the Skyrme interaction we h ave also applied the Hartree-Fock+BCS model with the state-dependent $\\delta$-pairing interaction. Potential energy surfaces and pairing properties have been compared for the both types of forces.

  5. The use of Pb, Sr, and Hg isotopes in Great Lakes precipitation as a tool for pollution source attribution

    Energy Technology Data Exchange (ETDEWEB)

    Sherman, Laura S., E-mail: lsaylors@umich.edu [University of Michigan, Department of Earth and Environmental Sciences, 1100 N. University Ave., Ann Arbor, MI 48109 (United States); Blum, Joel D. [University of Michigan, Department of Earth and Environmental Sciences, 1100 N. University Ave., Ann Arbor, MI 48109 (United States); Dvonch, J. Timothy [University of Michigan, Air Quality Laboratory, 1415 Washington Heights, Ann Arbor, MI 48109 (United States); Gratz, Lynne E. [University of Washington-Bothell, 18115 Campus Way NE, Bothell, WA 98011 (United States); Landis, Matthew S. [U.S. EPA, Office of Research and Development, Research Triangle Park, NC 27709 (United States)

    2015-01-01

    The anthropogenic emission and subsequent deposition of heavy metals including mercury (Hg) and lead (Pb) present human health and environmental concerns. Although it is known that local and regional sources of these metals contribute to deposition in the Great Lakes region, it is difficult to trace emissions from point sources to impacted sites. Recent studies suggest that metal isotope ratios may be useful for distinguishing between and tracing source emissions. We measured Pb, strontium (Sr), and Hg isotope ratios in daily precipitation samples that were collected at seven sites across the Great Lakes region between 2003 and 2007. Lead isotope ratios ({sup 207}Pb/{sup 206}Pb = 0.8062 to 0.8554) suggest that Pb deposition was influenced by coal combustion and processing of Mississippi Valley-Type Pb ore deposits. Regional differences in Sr isotope ratios ({sup 87}Sr/{sup 86}Sr = 0.70859 to 0.71155) are likely related to coal fly ash and soil dust. Mercury isotope ratios (δ{sup 202}Hg = − 1.13 to 0.13‰) also varied among the sites, likely due to regional differences in coal isotopic composition, and fractionation occurring within industrial facilities and in the atmosphere. These data represent the first combined characterization of Pb, Sr, and Hg isotope ratios in precipitation collected across the Great Lakes region. We demonstrate the utility of multiple metal isotope ratios in parallel with traditional trace element multivariate statistical modeling to enable more complete pollution source attribution. - Highlights: • We measured Pb, Sr, and Hg isotopes in precipitation from the Great Lakes region. • Pb isotopes suggest that deposition was impacted by coal combustion and metal production. • Sr isotope ratios vary regionally, likely due to soil dust and coal fly ash. • Hg isotopes vary due to fractionation occurring within facilities and the atmosphere. • Isotope results support conclusions of previous trace element receptor modeling.

  6. Stable isotope composition of human fingernails from Slovakia

    Energy Technology Data Exchange (ETDEWEB)

    Grolmusová, Zuzana, E-mail: zuzana.grolmusova@geology.sk [Comenius University in Bratislava, Faculty of Mathematics, Physics and Informatics, Department of Experimental Physics, Mlynská dolina F2, 842 48 Bratislava (Slovakia); State Geological Institute of Dionýz Štúr, Laboratory of Isotope Geology, Mlynská dolina 1, 817 04 Bratislava (Slovakia); Rapčanová, Anna [Comenius University in Bratislava, Faculty of Mathematics, Physics and Informatics, Department of Experimental Physics, Mlynská dolina F2, 842 48 Bratislava (Slovakia); Michalko, Juraj; Čech, Peter [State Geological Institute of Dionýz Štúr, Laboratory of Isotope Geology, Mlynská dolina 1, 817 04 Bratislava (Slovakia); Veis, Pavel [Comenius University in Bratislava, Faculty of Mathematics, Physics and Informatics, Department of Experimental Physics, Mlynská dolina F2, 842 48 Bratislava (Slovakia); State Geological Institute of Dionýz Štúr, Laboratory of Isotope Geology, Mlynská dolina 1, 817 04 Bratislava (Slovakia)

    2014-10-15

    Stable isotope composition of human fingernails has proven to be useful for documenting human dietary information and geographical patterns in archeological, forensic, anthropological and biological studies. Therefore, it is of interest to detect all factors influencing the stable isotopic composition in the certain regions in the world. Carbon and nitrogen isotope data of human fingernail keratin from 52 individuals from Slovakia were reported in this study. The online combustion and continuous flow isotope-ratio mass spectrometer Delta V Advantage was used for δ{sup 13}C and δ{sup 15}N analysis of fingernail keratin samples from 24 vegetarian and 28 omnivorous individuals. A group of people with frequent meat consumption showed enrichment in {sup 13}C and {sup 15}N isotopes in fingernails. A similar trend was observed with increasing seafood in an individual's diet. Moreover a significant difference was revealed between smokers and nonsmokers for both δ{sup 13}C and δ{sup 15}N values. These data were compared to previously published δ{sup 13}C and δ{sup 15}N fingernail values from across the globe. This study brings new information on the stable isotope signature of individuals from Slovakia and characterizes the Central European region for the first time. The stable isotope composition of fingernails is influenced by the frequency of meat and seafood consumption as well as smoking. - Highlights: • This study deals with stable isotope analyses of fingernails from Slovak volunteers. • δ{sup 13}C and δ{sup 15}N values of vegetarian and omnivore fingernails were compared. • Influence of sex, diet and smoking was studied.

  7. β-decay properties of neutron-deficient Pt, Hg, and Pb isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Sarriguren, P., E-mail: p.sarriguren@csic.es [Instituto de Estructura de la Materia, IEM-CSIC, Serrano 123, E-28006 Madrid (Spain); Boillos, J. M. [Instituto de Estructura de la Materia, IEM-CSIC, Serrano 123, E-28006 Madrid (Spain); Universidad de Santiago de Compostela, 15706 Santiago de Compostela (Spain); Moreno, O. [Center for Theoretical Physics, Laboratory for Nuclear Science and Department of Physics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States); Moya de Guerra, E. [Departamento de Física Atómica, Molecular y Nuclear, Facultad de Ciencias Físicas, Universidad Complutense de Madrid, E-28040 Madrid (Spain)

    2015-10-15

    Neutron-deficient isotopes in the lead region are well established examples of the shape coexistence phenomenon in nuclei. In this work, bulk and decay properties, including deformation energy curves, charge mean square radii, Gamow-Teller (GT) strength distributions, and β-decay half-lives, are studied in neutron-deficient Pt, Hg, and Pb isotopes. The nuclear structure involved is described microscopically from deformed quasiparticle random-phase approximation calculations with residual interactions in both particle-hole and particle-particle channels, performed on top of a self-consistent deformed quasiparticle Skyrme Hartree-Fock basis. The sensitivity to deformation of the GT strength distributions in those isotopes is proposed as an additional complementary signature of the nuclear shape. The β-decay half-lives resulting from the GT strength distributions are compared to experiment to demonstrate the ability of the method.

  8. Stable platinum isotope measurements in presolar nanodiamonds by TEAMS

    Energy Technology Data Exchange (ETDEWEB)

    Wallner, A., E-mail: anton.wallner@univie.ac.at [University of Vienna, Faculty of Physics, VERA Laboratory, Waehringer Strasse 17, A-1090 Vienna (Austria); Department of Nuclear Physics, Research School of Physics and Engineering, Australian National University, Canberra (Australia); Australian Nuclear Science and Technology Organisation (ANSTO), Lucas Heights (Australia); Melber, K. [University of Vienna, Faculty of Physics, VERA Laboratory, Waehringer Strasse 17, A-1090 Vienna (Austria); Merchel, S. [Helmholtz-Zentrum Dresden-Rossendorf (HZDR), D-01314 Dresden (Germany); Ott, U. [Max-Planck-Institut fuer Chemie, Joh.-J.-Becherweg 27, D-55128 Mainz (Germany); Forstner, O.; Golser, R.; Kutschera, W.; Priller, A.; Steier, P. [University of Vienna, Faculty of Physics, VERA Laboratory, Waehringer Strasse 17, A-1090 Vienna (Austria)

    2013-01-15

    Nanodiamonds are stardust grains commonly found in primitive meteorites. They survived the formation of the solar system and kept their own individuality. Measurements of trace-element isotopic signatures in these grains will help understanding heavy element nucleosynthesis in massive stars and dust formation from their ejecta. We have continued previous attempts to search for stable Pt isotope anomalies in nanodiamonds via trace element accelerator mass spectrometry (TEAMS). The installation of a new injector beam line at the VERA facility allowed studying low traces of stable elements in different materials. Moreover, recent experiments showed that VERA provides the required measurement precision together with a low Pt machine background. Here, we observed for the first time an indication for enhancements of {sup 198}Pt/{sup 195}Pt isotope ratios in two diamond residues prepared by different chemical separation techniques from the Allende meteorite. Variations in other isotopic ratios were within analytical uncertainty, and no anomaly was identified in a third diamond fraction.

  9. Stable platinum isotope measurements in presolar nanodiamonds by TEAMS

    Science.gov (United States)

    Wallner, A.; Melber, K.; Merchel, S.; Ott, U.; Forstner, O.; Golser, R.; Kutschera, W.; Priller, A.; Steier, P.

    2013-01-01

    Nanodiamonds are stardust grains commonly found in primitive meteorites. They survived the formation of the solar system and kept their own individuality. Measurements of trace-element isotopic signatures in these grains will help understanding heavy element nucleosynthesis in massive stars and dust formation from their ejecta. We have continued previous attempts to search for stable Pt isotope anomalies in nanodiamonds via trace element accelerator mass spectrometry (TEAMS). The installation of a new injector beam line at the VERA facility allowed studying low traces of stable elements in different materials. Moreover, recent experiments showed that VERA provides the required measurement precision together with a low Pt machine background. Here, we observed for the first time an indication for enhancements of 198Pt/195Pt isotope ratios in two diamond residues prepared by different chemical separation techniques from the Allende meteorite. Variations in other isotopic ratios were within analytical uncertainty, and no anomaly was identified in a third diamond fraction. PMID:23565017

  10. Stable platinum isotope measurements in presolar nanodiamonds by TEAMS

    Science.gov (United States)

    Wallner, A.; Melber, K.; Merchel, S.; Ott, U.; Forstner, O.; Golser, R.; Kutschera, W.; Priller, A.; Steier, P.

    2013-01-01

    Nanodiamonds are stardust grains commonly found in primitive meteorites. They survived the formation of the solar system and kept their own individuality. Measurements of trace-element isotopic signatures in these grains will help understanding heavy element nucleosynthesis in massive stars and dust formation from their ejecta. We have continued previous attempts to search for stable Pt isotope anomalies in nanodiamonds via trace element accelerator mass spectrometry (TEAMS). The installation of a new injector beam line at the VERA facility allowed studying low traces of stable elements in different materials. Moreover, recent experiments showed that VERA provides the required measurement precision together with a low Pt machine background. Here, we observed for the first time an indication for enhancements of 198Pt/195Pt isotope ratios in two diamond residues prepared by different chemical separation techniques from the Allende meteorite. Variations in other isotopic ratios were within analytical uncertainty, and no anomaly was identified in a third diamond fraction.

  11. Stable isotope geochemistry of East African waters. [Abstract only

    Energy Technology Data Exchange (ETDEWEB)

    Sayer, M.D.; Cerling, T.E.; Bowman, J.R.

    1983-03-01

    Lakes and Rivers in East Africa have varied stable isotopic compositions. Lakes exhibit enriched delta13-C values (-2 to +5%), while their inflowing rivers show depleted values (-15 to -8%). Hot springs and standing pools of water have intermediate values. Some small lakes are extremely variable in delta18-0 or deltaD (+2 to +8% and +20 to +40%, respectively for Lake Naivasha), whereas larger lakes are relatively constant for long periods of time (+5.6 to 6.1 and +36 to 40, respectively for Lake Turkana). Isotopic values are unrelated to salinity for comparison between lakes. Stable isotopes also reveal the sources of hot spring discharges: the Kapedo hot springs probably originate from Maralel and not from Lake Baringo as local legend has it; the hot springs north of Lake Naivasha are of meteoric origin while those to the south of Lake Naivasha have similar isotopic compositions to Lake Naivasha.

  12. Proliferation-resistant stable isotope separation based on optical pumping

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Cheol-Jung; Park, Hyunmin; Ko, Kwang-Hoon; Lim, Gwon; Kim, Taek-Soo; Rho, Sipyo; Cha, Yong-Ho; Han, Jamin; Jeong, Do-Young [Korea Atomic Energy Research Institute, Deajeon (Korea, Republic of). Quantum Optics Division

    2008-07-01

    Korea Atomic Energy Research Institute (KAERI) has developed the laser stable isotope separation based on optical pumping which can be applied to isotopes with small isotopic shift, provides high enrichment, is economical owing to high efficiency, and is proliferation-resistant. KAERI's laser isotope separation is based on isotope-selective optical pumping by very narrow bandwidth continuous wave laser followed by efficient infrared photo-ionization. KAERI demonstrated the pilot production of Tl-203 enriching over 97 % and separating 100 mg/hr. KAERI also demonstrated the separation of Yb-168 over 30 % and Yb- 176 over 97 % with tens of mg/hr. KAERI plans to scale up the production of Tl-203 up to 500 mg/hr and apply it to separation of Zn-67, Zn-70, Ba-130 and Ca-48 which are very important in medical industry and basic sciences. (author)

  13. Stable isotopes in leaf water of terrestrial plants.

    Science.gov (United States)

    Cernusak, Lucas A; Barbour, Margaret M; Arndt, Stefan K; Cheesman, Alexander W; English, Nathan B; Feild, Taylor S; Helliker, Brent R; Holloway-Phillips, Meisha M; Holtum, Joseph A M; Kahmen, Ansgar; McInerney, Francesca A; Munksgaard, Niels C; Simonin, Kevin A; Song, Xin; Stuart-Williams, Hilary; West, Jason B; Farquhar, Graham D

    2016-05-01

    Leaf water contains naturally occurring stable isotopes of oxygen and hydrogen in abundances that vary spatially and temporally. When sufficiently understood, these can be harnessed for a wide range of applications. Here, we review the current state of knowledge of stable isotope enrichment of leaf water, and its relevance for isotopic signals incorporated into plant organic matter and atmospheric gases. Models describing evaporative enrichment of leaf water have become increasingly complex over time, reflecting enhanced spatial and temporal resolution. We recommend that practitioners choose a model with a level of complexity suited to their application, and provide guidance. At the same time, there exists some lingering uncertainty about the biophysical processes relevant to patterns of isotopic enrichment in leaf water. An important goal for future research is to link observed variations in isotopic composition to specific anatomical and physiological features of leaves that reflect differences in hydraulic design. New measurement techniques are developing rapidly, enabling determinations of both transpired and leaf water δ(18) O and δ(2) H to be made more easily and at higher temporal resolution than previously possible. We expect these technological advances to spur new developments in our understanding of patterns of stable isotope fractionation in leaf water.

  14. Stable isotope techniques in plant water sources:a review

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    The stable hydrogen and oxygen isotopes widely exist in various kinds of natural water.Plants have to cope with various water sources:rainwater,soil water,groundwater,sea water,and mixtures.These are usually characterized by different isotopic signatures (18O/16O and D/H ratios).Because there are relative abundance variations in water,and plant roots do not discriminate against specific water isotopes during water uptake,hydrogen and oxygen stable isotope ratios of water within plants provide new information on water sources,interactions between plant species and water use patterns under natural conditions.At present,the measurement of δD,δ18O composition of various potential water sources and stem water has become significant means to identify plant water sources.Based on previous studies,this review highlights recent advances such as theory basis,methodology,as well as different spatial and temporal scales,and existed questions and prospects.Stable isotope techniques for estimating plant water sources have provided valuable tools for conducting basic and applied research.Future studies emphasize the modification of preparing methods,isotope technique combined with other measurements,and aerial organs of plant water source should be en-couraged.

  15. Modelling of stable water isotopes in Central Europe with COSMOiso

    Science.gov (United States)

    Christner, Emanuel; Pfahl, Stephan; Schädler, Gerd

    2016-04-01

    Atmospheric water in form of vapor or clouds is responsible for ˜75 % of the natural greenhouse effect and carries huge amounts of latent heat. For this reason, a best possible description of the hydrological cycle is a prerequisite for reliable climate modelling. As the stable isotopes H216O, H218O and HDO differ in vapor pressure, they are fractionated during phase changes and contain information about the formation of precipitation, evaporation from the ground, etc. Therefore, the isotopic composition of atmospheric water is an useful tracer to test and improve our understanding of the extremely complex and variable hydrological cycle in Earth's atmosphere. Within the project PalMod the isotope-enabled limited-area model COSMOiso will be used for high-resolution isotope simulations of paleo-climates. For validation with modern observations we compare 12 years of modelled isotope ratios from Central Europe to observations of the Global Network of Isotopes in Precipitation (GNIP) and to observations of isotope ratios of water vapor at different locations in Germany. We find a good agreement of modelled and observed isotope ratios in summer. In winter, we observe a systematic overestimation of modelled isotope ratios in precipitation and low-level water vapor. We relate those differences to specific circulation regimes with predominantly easterly moisture transport and the corresponding strong dependence of modelled isotope ratios on lateral boundary data. Furthermore, we investigate the dependence of modelled isotope ratios in winter on the type of isotope fractionation during surface evaporation at skin temperatures close to the freezing point.

  16. Spatial distribution of stable water isotopes in alpine snow cover

    Directory of Open Access Journals (Sweden)

    N. Dietermann

    2013-07-01

    Full Text Available The aim of this study was to analyse and predict the mean stable water isotopic composition of the snow cover at specific geographic locations and altitudes. In addition, the dependence of the isotopic composition of the entire snow cover on altitude was analysed. Snow in four Swiss catchments was sampled at the end of the accumulation period in April 2010 and a second time during snowmelt in May 2010 and analysed for stable isotope composition of 2H and 18O. The sampling was conducted at both south-facing and north-facing slopes at elevation differences of 100 m, for a total altitude difference of approximately 1000 m. The observed variability of isotopic composition of the snow cover was analysed with stepwise multiple linear regression models. The analysis indicated that there is only a limited altitude effect on the isotopic composition when considering all samples. This is due to the high variability of the isotopic composition of the precipitation during the winter months and, in particular in the case of south-facing slopes, an enrichment of heavy isotopes due to intermittent melting processes. This enrichment effect could clearly be observed in the samples which were taken later in the year. A small altitudinal gradient of the isotopic composition could only be observed at some north-facing slopes. However, the dependence of snow depth and the day of the year were significant predictor variables in all models. This study indicates the necessity to further study the variability of water isotopes in the snow cover to increase prediction for isotopic composition of snowmelt and hence increase model performance of residence time models for alpine areas in order to better understand the accumulation processes and the sources of water in the snow cover of high mountains.

  17. [Progress in stable isotope labeled quantitative proteomics methods].

    Science.gov (United States)

    Zhou, Yuan; Shan, Yichu; Zhang, Lihua; Zhang, Yukui

    2013-06-01

    Quantitative proteomics is an important research field in post-genomics era. There are two strategies for proteome quantification: label-free methods and stable isotope labeling methods which have become the most important strategy for quantitative proteomics at present. In the past few years, a number of quantitative methods have been developed, which support the fast development in biology research. In this work, we discuss the progress in the stable isotope labeling methods for quantitative proteomics including relative and absolute quantitative proteomics, and then give our opinions on the outlook of proteome quantification methods.

  18. Decay studies and mass measurements on isobarically pure neutron-rich Hg and Tl isotopes

    CERN Multimedia

    Schweikhard, L C; Savreux, R P; Hager, U D K; Beck, D; Blaum, K

    2007-01-01

    We propose to perform mass measurements followed by $\\beta$- and $\\gamma$-decay studies on isobarically pure beams of neutron-rich Hg and Tl isotopes, which are very poorly known due to a large contamination at ISOL-facilities with surface-ionised francium. The aim is to study the binding energies of mother Hg and Tl nuclides, as well as the energies, spins and parities of the excited and ground states in the daughter Tl and Pb isotopes. The proposed studies will address a new subsection of the nuclear chart, with Z 126, where only 9 nuclides have been observed so far. Our studies will provide valuable input for mass models and shell-model calculations: they will probe the proton hole-neutron interaction and will allow to refine the matrix elements for the two-body residual interaction. Furthermore, they also give prospects for discovering new isomeric states or even new isotopes, for which the half-lives are predicted in the minute- and second-range.\\\\ To reach the isobaric purity, the experiments will be p...

  19. Shape coexistence in neutron-deficient Hg isotopes studied via lifetime measurements in $^{184,186}$Hg and two-state mixing calculations

    CERN Document Server

    Gaffney, L P; Page, R.D.; Grahn, T.; Scheck, M.; Butler, P.A.; Bertone, P.F.; Bree, N.; Carroll, R.J.; Carpenter, M.P.; Chiara, C.J.; Dewald, A.; Filmer, F.; Fransen, C.; Huyse, M.; Janssens, R.V.F.; Joss, D.T.; Julin, R.; Kondev, F.G.; Nieminen, P.; Pakarinen, J.; Rigby, S.V.; Rother, W.; Van Duppen, P.; Watkins, H.V.; Wrzosek-Lipska, K.; Zhu, S.

    2014-01-01

    The neutron-deficient mercury isotopes, $^{184,186}$Hg, were studied with the Recoil Distance Doppler Shift (RDDS) method using the Gammasphere array and the K\\"oln Plunger device. The Differential Decay Curve Method (DDCM) was employed to determine the lifetimes of the yrast states in $^{184,186}$Hg. An improvement on previously measured values of yrast states up to $8^{+}$ is presented as well as first values for the $9_{3}$ state in $^{184}$Hg and $10^{+}$ state in $^{186}$Hg. $B(E2)$ values are calculated and compared to a two-state mixing model which utilizes the variable moment of inertia (VMI) model, allowing for extraction of spin-dependent mixing strengths and amplitudes.

  20. Variation in terrestrial and aquatic sources of methylmercury in stream predators as revealed by stable mercury isotopes.

    Science.gov (United States)

    Tsui, Martin Tsz-Ki; Blum, Joel D; Finlay, Jacques C; Balogh, Steven J; Nollet, Yabing H; Palen, Wendy J; Power, Mary E

    2014-09-02

    Mercury (Hg) is widely distributed in the environment, and its organic form, methylmercury (MeHg), can extensively bioaccumulate and biomagnify in aquatic and terrestrial food webs. Concentrations of MeHg in organisms are highly variable, and the sources in natural food webs are often not well understood. This study examined stable isotope ratios of MeHg (mass-dependent fractionation, as δ(202)HgMeHg; and mass-independent fractionation, as Δ(199)HgMeHg) in benthic invertebrates, juvenile steelhead trout (Oncorhynchus mykiss), and water striders (Gerris remigis) along a stream productivity gradient, as well as carnivorous terrestrial invertebrates, in a forested watershed at the headwater of South Fork Eel River in northern California. Throughout the sampling sites, δ(202)HgMeHg (after correction due to the effect of MeHg photodegradation) was significantly different between benthic (median = -1.40‰; range, -2.34 to -0.78‰; total number of samples = 29) and terrestrial invertebrates (median = +0.51‰; range, -0.37 to +1.40‰; total number of samples = 9), but no major difference between these two groups was found for Δ(199)HgMeHg. Steelhead trout (52 individual fishes) have MeHg of predominantly aquatic origins, with a few exceptions at the upstream locations (e.g., 1 fish collected in a tributary had a purely terrestrial MeHg source and 4 fishes had mixed aquatic and terrestrial MeHg sources). Water striders (seven pooled samples) derive MeHg largely from terrestrial sources throughout headwater sections. These data suggest that direct terrestrial subsidy (e.g., terrestrial invertebrates falling into water) can be important for some stream predators in headwater streams and could represent an important means of transfer of terrestrially derived MeHg (e.g., in situ methylation within forests, atmospheric sources) to aquatic ecosystems. Moreover, these findings show that terrestrial subsidies can enhance MeHg bioaccumulation of consumers in headwater

  1. Heavy element stable isotope ratios: analytical approaches and applications.

    Science.gov (United States)

    Tanimizu, Masaharu; Sohrin, Yoshiki; Hirata, Takafumi

    2013-03-01

    Continuous developments in inorganic mass spectrometry techniques, including a combination of an inductively coupled plasma ion source and a magnetic sector-based mass spectrometer equipped with a multiple-collector array, have revolutionized the precision of isotope ratio measurements, and applications of inorganic mass spectrometry for biochemistry, geochemistry, and marine chemistry are beginning to appear on the horizon. Series of pioneering studies have revealed that natural stable isotope fractionations of many elements heavier than S (e.g., Fe, Cu, Zn, Sr, Ce, Nd, Mo, Cd, W, Tl, and U) are common on Earth, and it had been widely recognized that most physicochemical reactions or biochemical processes induce mass-dependent isotope fractionation. The variations in isotope ratios of the heavy elements can provide new insights into past and present biochemical and geochemical processes. To achieve this, the analytical community is actively solving problems such as spectral interference, mass discrimination drift, chemical separation and purification, and reduction of the contamination of analytes. This article describes data calibration and standardization protocols to allow interlaboratory comparisons or to maintain traceability of data, and basic principles of isotope fractionation in nature, together with high-selectivity and high-yield chemical separation and purification techniques for stable isotope studies.

  2. STABLE CARBON ISOTOPE ANALYSIS OF SUBFOSSIL WOOD FROM AUSTRIAN ALPS

    Science.gov (United States)

    KŁUSEK, MARZENA; PAWEŁCZYK, SŁAWOMIRA

    2015-01-01

    The presented studies were carried out in order to check the usefulness of subfossil wood for stable isotope analysis. The aim of research was also to define the optimal method of subfossil samples preparation. Subfossil samples used during the presented studies are a part of the multi-century dendrochronological scale. This chronology originates in an area situated around a small mountain lake — Schwarzersee, in Austria. The obtained results of stable carbon isotope measurements confirmed that the method of α-cellulose extraction by the application of acidic sodium chlorite and sodium hydroxide solutions removes resins and other mobile compounds from wood. Therefore, in the case of the analysed samples, the additional chemical process of extractives removing was found to be unnecessary. Studied wood samples contained an adequate proportion of α-cellulose similar to the values characteristic for the contemporary trees. This proved an adequate wood preservation which is essential for the conduction of isotopic research. PMID:26346297

  3. Stereoselective synthesis of stable-isotope-labeled amino acids

    Energy Technology Data Exchange (ETDEWEB)

    Unkefer, C.J.; Martinez, R.A.; Silks, L.A. III [Los Alamos National Laboratory, NM (United States); Lodwig, S.N. [Centralia College, WA (United States)

    1994-12-01

    For magnetic resonance and vibrational spectroscopies to reach their full potential, they must be used in combination with sophisticated site-specific stable isotope labeling of biological macromolecules. Labeled amino acids are required for the study of the structure and function of enzymes and proteins. Because there are 20 common amino acids, each with its own distinguishing chemistry, they remain a synthetic challenge. The Oppolzer chiral auxiliary provides a general tool with which to approach the synthesis of labeled amino acids. By using the Oppolzer auxiliary, amino acids can be constructed from several small molecules, which is ideal for stable isotope labeling. In addition to directing the stereochemistry at the {alpha}-carbon, the camphorsultam can be used for stereo-specific isotope labeling at prochiral centers in amino acids. By using the camphorsultam auxiliary we have the potential to synthesize virtually any isotopomer of all of the common amino acids.

  4. Biomedical research applications of electromagnetically separated enriched stable isotopes

    Science.gov (United States)

    Lambrecht, R. M.

    The current and projected annual requirements through 1985 for stable isotopes enriched by electromagnetic separation methods were reviewed for applications in various types of biomedical research: (1) medical radiosiotope production, labeled compounds, and potential radio-pharmaceuticals; (2) nutrition, food science, and pharmacology: (3) metallobiochemistry and environmental toxicology; (4) nuclear magnetic resonance, electron paramagnetic resonance, and moessbauer spectroscopy in biochemical, biophysical, and biomedical research; and (5) miscellaneous advances in radioactive and nonradioactive tracer technology. Radioisotopes available from commercial sources or routinely used in clinical nuclear medicine were excluded. Current requirements for enriched stable isotopes in biomedical research are not being satisfied. Severe shortages exist for Mg 26, Ca 43, Zn 70, Se 76, Se 77, Se 78, Pd 102, Cd 111, Cd 113, and Os 190. Many interesting and potentially important investigations in biomedical research require small quantities of specific elements at high isotopic enrichments.

  5. Fractionation of Stable Isotopes in Atmospheric Aerosol Reactions

    DEFF Research Database (Denmark)

    Meusinger, Carl

    reactions and undergo complex chemical and physical changes during their lifetimes. In order to assess processes that form and alter aerosols, information provided by stable isotopes can be used to help constrain estimates on the strength of aerosol sources and sinks. This thesis studies (mass......-independent) fractionation processes of stable isotopes of C, N, O and S in order to investigate three different systems related to aerosols: 1. Post-depositional processes of nitrate in snow that obscure nitrate ice core records 2. Formation and aging of secondary organic aerosol generated by ozonolysis of X...... as required. The kndings provide important results for the studies' respective felds, including a description of the isotopic fractionation and quantum yield of nitrate photolysis in snow, equilibrium fractionation in secondary organic aerosol and fractionation constants of different oxidation pathways of SO2....

  6. Endogenous surfactant turnover in preterm infants measured with stable isotopes

    NARCIS (Netherlands)

    J.E.H. Bunt (Jan Erik); L.J.I. Zimmermann (Luc); J.L.D. Wattimena (Josias); R.H.Th. van Beek (Ron); P.J.J. Sauer (Pieter); V.P. Carnielli (Virgilio)

    1998-01-01

    textabstractWe studied surfactant synthesis and turnover in vivo in preterm infants using the stable isotope [U-13C]glucose, as a precursor for the synthesis of palmitic acid in surfactant phosphatidylcholine (PC). Six preterm infants (birth weight, 916 +/- 244 g; gesta

  7. Apparatus and method for monitoring of gas having stable isotopes

    Science.gov (United States)

    Clegg, Samuel M; Fessenden-Rahn, Julianna E

    2013-03-05

    Gas having stable isotopes is monitored continuously by using a system that sends a modulated laser beam to the gas and collects and transmits the light not absorbed by the gas to a detector. Gas from geological storage, or from the atmosphere can be monitored continuously without collecting samples and transporting them to a lab.

  8. STABLE ISOTOPES AS INDICATORS OF SOIL WATER DYNAMICS IN WATERSHEDS

    Science.gov (United States)

    Stream water quality and quantity depend on discharge rates of water and nutrients from soils. However, soil-water storage is very dynamic and strongly influenced by plants. We analyzed stable isotopes of oxygen and hydrogen to quantify spatial and temporal changes in evaporati...

  9. Development of stable isotope mixing models in ecology - Fremantle

    Science.gov (United States)

    More than 40 years ago, stable isotope analysis methods used in geochemistry began to be applied to ecological studies. One common application is using mathematical mixing models to sort out the proportional contributions of various sources to a mixture. Examples include contri...

  10. Development of stable isotope mixing models in ecology - Dublin

    Science.gov (United States)

    More than 40 years ago, stable isotope analysis methods used in geochemistry began to be applied to ecological studies. One common application is using mathematical mixing models to sort out the proportional contributions of various sources to a mixture. Examples include contri...

  11. Historical development of stable isotope mixing models in ecology

    Science.gov (United States)

    More than 40 years ago, stable isotope analysis methods used in geochemistry began to be applied to ecological studies. One common application is using mathematical mixing models to sort out the proportional contributions of various sources to a mixture. Examples include contri...

  12. Development of stable isotope mixing models in ecology - Sydney

    Science.gov (United States)

    More than 40 years ago, stable isotope analysis methods used in geochemistry began to be applied to ecological studies. One common application is using mathematical mixing models to sort out the proportional contributions of various sources to a mixture. Examples include contri...

  13. Development of stable isotope mixing models in ecology - Perth

    Science.gov (United States)

    More than 40 years ago, stable isotope analysis methods used in geochemistry began to be applied to ecological studies. One common application is using mathematical mixing models to sort out the proportional contributions of various sources to a mixture. Examples include contri...

  14. Subterranean sympatry: An investigation into diet using stable isotope analysis

    CSIR Research Space (South Africa)

    Robb, GN

    2012-11-01

    Full Text Available mole-rat (Georychus capensis) and the Cape dune mole-rat (Bathyergus suillus) were examined using stable isotope analysis. Blood, fur and claw samples were collected from 70 mole-rats, in addition to several potential food items, to assess food...

  15. COMBINING SOURCES IN STABLE ISOTOPE MIXING MODELS: ALTERNATIVE METHODS

    Science.gov (United States)

    Stable isotope mixing models are often used to quantify source contributions to a mixture. Examples include pollution source identification; trophic web studies; analysis of water sources for soils, plants, or water bodies; and many others. A common problem is having too many s...

  16. Apparatus and method for monitoring of gas having stable isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Clegg, Samuel M; Fessenden-Rahn, Julianna E

    2013-03-05

    Gas having stable isotopes is monitored continuously by using a system that sends a modulated laser beam to the gas and collects and transmits the light not absorbed by the gas to a detector. Gas from geological storage, or from the atmosphere can be monitored continuously without collecting samples and transporting them to a lab.

  17. Stable isotopes in the diagnosis and treatment of inherited hyperammonemia

    Science.gov (United States)

    Mew, Nicholas Ah; Yudkoff, Marc; Tuchman, Mendel

    2014-01-01

    Stable isotopes have greatly contributed to our understanding of nitrogen metabolism and the urea cycle. The measurement of urea flux via isotopic methods has traditionally been utilized to determine total body protein synthesis in subjects with an intact urea cycle. However, isotopic studies of nitrogen metabolism are also a useful adjunct to conventional clinical investigations in the diagnosis and management of the inherited hyperammonemias. Such studies offer a safe non-invasive method of measuring the reduction of in vivo hepatic ureagenesis, and thus may provide a more accurate measure of phenotypic severity in affected patients. In addition, isotopic methods are ideally suited to evaluate the efficacy of novel therapies to augment urea production. PMID:24634704

  18. Sources of mercury to San Francisco Bay surface sediment as revealed by mercury stable isotopes

    Science.gov (United States)

    Gehrke, Gretchen E.; Blum, Joel D.; Marvin-DePasquale, Mark

    2011-01-01

    Mercury (Hg) concentrations and isotopic compositions were examined in shallow-water surface sediment (0–2 cm) from San Francisco (SF) Bay to determine the extent to which historic Hg mining contributes to current Hg contamination in SF Bay, and to assess the use of Hg isotopes to trace sources of contamination in estuaries. Inter-tidal and wetland sediment had total Hg (HgT) concentrations ranging from 161 to 1529 ng/g with no simple gradients of spatial variation. In contrast, inter-tidal and wetland sediment displayed a geographic gradient of δ202Hg values, ranging from -0.30% in the southern-most part of SF Bay (draining the New Almaden Hg District) to -0.99% in the northern-most part of SF Bay near the Sacramento–San Joaquin River Delta. Similar to SF Bay inter-tidal sediment, surface sediment from the Alviso Slough channel draining into South SF Bay had a δ202Hg value of -0.29%, while surface sediment from the Cosumnes River and Sacramento–San Joaquin River Delta draining into north SF Bay had lower average δ202Hg values of -0.90% and -0.75%, respectively. This isotopic trend suggests that Hg-contaminated sediment from the New Almaden Hg District mixes with Hg-contaminated sediment from a low δ202Hg source north of SF Bay. Tailings and thermally decomposed ore (calcine) from the New Idria Hg mine in the California Coast Range had average δ202Hg values of -0.37 and +0.03%, respectively, showing that Hg calcination fractionates Hg isotopes resulting in Hg contamination from Hg(II) mine waste products with higher δ202Hg values than metallic Hg(0) produced from Hg mines. Thus, there is evidence for at least two distinct isotopic signals for Hg contamination in SF Bay: Hg associated with calcine waste materials at Hg mines in the Coast Range, such as New Almaden and New Idria; and Hg(0) produced from these mines and used in placer gold mines and/or in other industrial processes in the Sierra Nevada region and SF Bay area.

  19. Sources of mercury to San Francisco Bay surface sediment as revealed by mercury stable isotopes

    Science.gov (United States)

    Gehrke, Gretchen E.; Blum, Joel D.; Marvin-DiPasquale, Mark

    2011-02-01

    Mercury (Hg) concentrations and isotopic compositions were examined in shallow-water surface sediment (0-2 cm) from San Francisco (SF) Bay to determine the extent to which historic Hg mining contributes to current Hg contamination in SF Bay, and to assess the use of Hg isotopes to trace sources of contamination in estuaries. Inter-tidal and wetland sediment had total Hg (Hg T) concentrations ranging from 161 to 1529 ng/g with no simple gradients of spatial variation. In contrast, inter-tidal and wetland sediment displayed a geographic gradient of δ 202Hg values, ranging from -0.30‰ in the southern-most part of SF Bay (draining the New Almaden Hg District) to -0.99‰ in the northern-most part of SF Bay near the Sacramento-San Joaquin River Delta. Similar to SF Bay inter-tidal sediment, surface sediment from the Alviso Slough channel draining into South SF Bay had a δ 202Hg value of -0.29‰, while surface sediment from the Cosumnes River and Sacramento-San Joaquin River Delta draining into north SF Bay had lower average δ 202Hg values of -0.90‰ and -0.75‰, respectively. This isotopic trend suggests that Hg-contaminated sediment from the New Almaden Hg District mixes with Hg-contaminated sediment from a low δ 202Hg source north of SF Bay. Tailings and thermally decomposed ore (calcine) from the New Idria Hg mine in the California Coast Range had average δ 202Hg values of -0.37 and +0.03‰, respectively, showing that Hg calcination fractionates Hg isotopes resulting in Hg contamination from Hg(II) mine waste products with higher δ 202Hg values than metallic Hg(0) produced from Hg mines. Thus, there is evidence for at least two distinct isotopic signals for Hg contamination in SF Bay: Hg associated with calcine waste materials at Hg mines in the Coast Range, such as New Almaden and New Idria; and Hg(0) produced from these mines and used in placer gold mines and/or in other industrial processes in the Sierra Nevada region and SF Bay area.

  20. Inferring foliar water uptake using stable isotopes of water.

    Science.gov (United States)

    Goldsmith, Gregory R; Lehmann, Marco M; Cernusak, Lucas A; Arend, Matthias; Siegwolf, Rolf T W

    2017-07-22

    A growing number of studies have described the direct absorption of water into leaves, a phenomenon known as foliar water uptake. The resultant increase in the amount of water in the leaf can be important for plant function. Exposing leaves to isotopically enriched or depleted water sources has become a common method for establishing whether or not a plant is capable of carrying out foliar water uptake. However, a careful inspection of our understanding of the fluxes of water isotopes between leaves and the atmosphere under high humidity conditions shows that there can clearly be isotopic exchange between the two pools even in the absence of a change in the mass of water in the leaf. We provide experimental evidence that while leaf water isotope ratios may change following exposure to a fog event using water with a depleted oxygen isotope ratio, leaf mass only changes when leaves are experiencing a water deficit that creates a driving gradient for the uptake of water by the leaf. Studies that rely on stable isotopes of water as a means of studying plant water use, particularly with respect to foliar water uptake, must consider the effects of these isotopic exchange processes.

  1. Evaluating the role of re-adsorption of dissolved Hg(2+) during cinnabar dissolution using isotope tracer technique.

    Science.gov (United States)

    Jiang, Ping; Li, Yanbin; Liu, Guangliang; Yang, Guidi; Lagos, Leonel; Yin, Yongguang; Gu, Baohua; Jiang, Guibin; Cai, Yong

    2016-11-05

    Cinnabar dissolution is an important factor controlling mercury (Hg) cycling. Recent studies have suggested the co-occurrence of re-adsorption of the released Hg during the course of cinnabar dissolution. However, there is a lack of feasible techniques that can quantitatively assess the amount of Hg re-adsorbed on cinnabar when investigating cinnabar dissolution. In this study, a new method, based on isotope tracing and dilution techniques, was developed to study the role of Hg re-adsorption in cinnabar dissolution. The developed method includes two key components: (1) accurate measurement of both released and spiked Hg in aqueous phase and (2) estimation of re-adsorbed Hg on cinnabar surface via the reduction in spiked (202)Hg(2+). By adopting the developed method, it was found that the released Hg for trials purged with oxygen could reach several hundred μgL(-1), while no significant cinnabar dissolution was detected under anaerobic condition. Cinnabar dissolution rate when considering Hg re-adsorption was approximately 2 times the value calculated solely with the Hg detected in the aqueous phase. These results suggest that ignoring the Hg re-adsorption process can significantly underestimate the importance of cinnabar dissolution, highlighting the necessity of applying the developed method in future cinnabar dissolution studies.

  2. Stable Isotope Mapping of Alaskan Grasses and Marijuana

    Science.gov (United States)

    Booth, A. L.; Wooller, M. J.

    2008-12-01

    The spatial variation of isotope signatures in organic material is a useful forensic tool, particularly when applied to the task of tracking the production and distribution of plant-derived illicit drugs. In order to identify the likely grow-locations of drugs such as marijuana from unknown locations (i.e., confiscated during trafficking), base isotope maps are needed that include measurements of plants from known grow-locations. This task is logistically challenging in remote, large regions such as Alaska. We are therefore investigating the potential of supplementing our base (marijuana) isotope maps with data derived from other plants from known locations and with greater spatial coverage in Alaska. These currently include >150 samples of modern C3 grasses (Poaceae) as well as marijuana samples (n = 18) from known grow-locations across the state. We conducted oxygen, carbon and nitrogen stable isotope analyses of marijuana and grasses (Poaceae). Poaceae samples were obtained from the University of Alaska Fairbanks (UAF) Museum of the North herbarium collection, originally collected by field botanists from around Alaska. Results indicate that the oxygen isotopic composition of these grasses range from 10‰ to 30‰, and broadly mirror the spatial pattern of water isotopes in Alaska. Our marijuana samples were confiscated around the state of Alaska and supplied to us by the UAF Police Department. δ13C, δ15N and δ18O values exhibit geographic patterns similar to the modern grasses, but carbon and nitrogen isotopes of some marijuana plants appear to be influenced by additional factors related to indoor growing conditions (supplementary CO2 sources and the application of organic fertilizer). As well as providing a potential forensic resource, our Poaceae isotope maps could serve additional value by providing resources for studying ecosystem nutrient cycling, for tracing natural ecological processes (i.e., animal migration and food web dynamics) and providing

  3. Stable isotope measurements of evapotranspiration partitioning in a maize field

    Science.gov (United States)

    Hogan, Patrick; Parajka, Juraj; Oismüller, Markus; Strauss, Peter; Heng, Lee; Blöschl, Günter

    2017-04-01

    Evapotranspiration (ET) is one of the most important processes in describing land surface - atmosphere interactions as it connects the energy and water balances. Furthermore knowledge of the individual components of evapotranspiration is important for ecohydrological modelling and agriculture, particularly for irrigation efficiency and crop productivity. In this study, we tested the application of the stable isotope method for evapotranspiration partitioning to a maize crop during the vegetative stage, using sap flow sensors as a comparison technique. Field scale ET was measured using an eddy covariance device and then partitioned using high frequency in-situ measurements of the isotopic signal of the canopy water vapor. The fraction of transpiration (Ft) calculated with the stable isotope method showed good agreement with the sap flow method. High correlation coefficient values were found between the two techniques, indicating the stable isotope method can successfully be applied in maize. The results show the changes in transpiration as a fraction of evapotranspiration after rain events and during the subsequent drying conditions as well as the relationship between transpiration and solar radiation and vapor pressure deficit.

  4. Patterns in Stable Isotope Values of Nitrogen and Carbon in ...

    Science.gov (United States)

    Stable isotope measurements of nitrogen and carbon (15N, 13ddC) are often used to characterize estuarine, nearshore, and open ocean ecosystems. Reliable information about the spatial distribution of base-level stable isotope values, often represented by primary producers, is critical to interpreting values in these ecosystems. While base-level isotope data are generally readily available for estuaries, nearshore coastal waters, and the open ocean, the continental shelf is less studied. To address this, and as a first step toward developing a surrogate for base-level isotopic signature in this region, we collected surface and deep water samples from the United States’ eastern continental shelf in the Western Atlantic Ocean, from the Gulf of Maine to Cape Hatteras, periodically between 2000 and 2013. During the study, particulate matter 15dN values ranged from 0.8 to 17.4‰, and 13dC values from −26.4 to −15.6‰over the region. We used spatial autocorrelation analysis and random forest modeling to examine the spatial trends and potential environmental drivers of the stable isotope values. We observed general trends toward lower values for both nitrogen and carbon isotopes at the seaward edge of the shelf. Conversely, higher 15dN and 13dC values were observed on the landward edge of the shelf, in particular in the southern portion of the sampling area. Across all sites, the magnitude of the difference between the 15dN of subsurface and surface particulate m

  5. Stable isotope patterns in micronekton from the Mozambique Channel

    Science.gov (United States)

    Ménard, Frédéric; Benivary, Hermann Doris; Bodin, Nathalie; Coffineau, Nathalie; Le Loc'h, François; Mison, Thomas; Richard, Pierre; Potier, Michel

    2014-02-01

    We measured the stable carbon (δ13C) and nitrogen (δ15N) isotopic composition of tissues of micronektonic organisms (fishes, squids, crustaceans and gelatinous organisms) collected in the Mozambique Channel during two scientific cruises in 2008 and 2009. The oceanic circulation in the Mozambique Channel is dominated by mesoscale cyclonic and anticyclonic eddies which play a key role in biological processes of less-productive deep-sea ecosystems. We investigated the potential impact of mesoscale features on the δ13C and δ15N values of 32 taxa of micronekton. Fishes, squids, crustaceans and gelatinous organisms encompassed a wide range of isotopic niches, with large overlaps among species. Our results showed that mesoscale features did not really influence the isotopic signatures of the sampled organisms, although cyclonic eddies can occasionally impact the nitrogen signatures of micronekton. We show that δ13C values were intermediate between standard offshore and nearshore signatures, suggesting that pelagic production in the Mozambique Channel could be partly supported by the transport and export of inorganic and organic particles from the Mozambican coast toward the offshore area. Trophic levels calculated from δ15N values ranged from 2.6 to 4.2, showing that micronekton taxa can be tertiary consumers in the Mozambique Channel. Our findings evidenced clusters of micronektonic organisms according to their δ15N or δ13C isotopic signatures, but variations in stable isotope values reflect a complex set of embedded processes linked to physical mesoscale dynamics (rotational dynamics of eddies) and basic biology and ecology of micronektonic organisms (vertical habitat, migration pattern, dietary habits, body length) that are discussed with regard to the stable isotope method based on time-integrated assimilated food.

  6. Stable carbon isotope ratios of ambient aromatic volatile organic compounds

    Science.gov (United States)

    Kornilova, Anna; Huang, Lin; Saccon, Marina; Rudolph, Jochen

    2016-09-01

    Measurements of mixing ratios and stable carbon isotope ratios of aromatic volatile organic compounds (VOC) in the atmosphere were made in Toronto (Canada) in 2009 and 2010. Consistent with the kinetic isotope effect for reactions of aromatic VOC with the OH radical the observed stable carbon isotope ratios are on average significantly heavier than the isotope ratios of their emissions. The change of carbon isotope ratio between emission and observation is used to determine the extent of photochemical processing (photochemical age, ∫ [OH]dt) of the different VOC. It is found that ∫ [OH]dt of different VOC depends strongly on the VOC reactivity. This demonstrates that for this set of observations the assumption of a uniform ∫ [OH]dt for VOC with different reactivity is not justified and that the observed values for ∫ [OH]dt are the result of mixing of VOC from air masses with different values for ∫ [OH]dt. Based on comparison between carbon isotope ratios and VOC concentration ratios it is also found that the varying influence of sources with different VOC emission ratios has a larger impact on VOC concentration ratios than photochemical processing. It is concluded that for this data set the use of VOC concentration ratios to determine ∫ [OH]dt would result in values for ∫ [OH]dt inconsistent with carbon isotope ratios and that the concept of a uniform ∫ [OH]dt for an air mass has to be replaced by the concept of individual values of an average ∫ [OH]dt for VOC with different reactivity.

  7. An Approach to Analysis of Stable Isotopes in Microsamples

    Institute of Scientific and Technical Information of China (English)

    韩友科; 安娜

    1991-01-01

    A new aproach to isotopic analysis of carbon,oxygen,sulfur and nitrogen in microsamples has been established.Samples were conventionally prepared by mixing microsamples to be analyzed with reference samples with known δ values in a proper proportion,and then analyzed for their stable isotopes as those at ordinary levels.According to the equilibrium relationships before and after mixing,the δvalues of unknown microsamples were calculated.The δ15N of the atmosphere was estimated at zero by this approach,which is concordant with the internationally recommended value.

  8. Infrared Spectroscopy and Stable Isotope Geochemistry of Hydrous Silicate Glasses

    Energy Technology Data Exchange (ETDEWEB)

    Stolper, Edward

    2007-03-05

    The focus of this DOE-funded project has been the study of volatile components in magmas and the atmosphere. Over the twenty-one year period of this project, we have used experimental petrology and stable isotope geochemistry to study the behavior and properties of volatile components dissolved in silicate minerals and melts and glasses. More recently, we have also studied the concentration and isotopic composition of CO2 in the atmosphere, especially in relation to air quality issues in the Los Angeles basin.

  9. Hg Isotopes Reveal Importance of In-Stream Processing and Legacy Inputs in East Fork Poplar Creek, Oak Ridge, Tennessee, USA.

    Science.gov (United States)

    Demers, J. D.; Blum, J. D.; Brooks, S. C.; Donovan, P. M.; Gu, B.; Riscassi, A.

    2015-12-01

    Understanding how mercury (Hg) contaminated ecosystems will recover as atmospheric emissions and industrial point source discharges are controlled has become a driving motivation of mercury research. Key to predicting recovery of mercury contaminated ecosystems is an understanding of the mobilization of legacy Hg sources, and the subsequent bioavailability and biogeochemical cycling of mobilized Hg within aquatic ecosystems. Herein, we utilize natural abundance stable Hg isotope techniques to place new constraints on mercury sources, transport, and transformations along the flow path of East Fork Poplar Creek (EFPC), Oak Ridge, Tennessee. The isotopic composition of mercury in stream water and suspended sediment along the flow path suggest that: (1) physical processes such as dilution and sedimentation cannot fully explain decreases in total mercury concentrations along the flow path and that in-stream processes may be more important than previously realized; (2) in-stream processes include photochemical transformations (~20%), but microbial reduction is likely more dominant (~80%); and (3) additional sources of mercury inputs to EFPC at base-flow may predominantly arise from the hyporheic zone during the growing season, with adjacent riparian wetlands and non-point-source impacted tributaries increasing in importance during the dormant season when the stream channel is more hydrologically connected to the watershed.

  10. Evaluating the role of re-adsorption of dissolved Hg{sup 2+} during cinnabar dissolution using isotope tracer technique

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, Ping [Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199 (United States); Li, Yanbin [Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education/Qingdao Collaborative Innovation Center of Marine Science and Technology, Ocean University of China, Qingdao 266100 (China); Liu, Guangliang [Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199 (United States); Southeast Environmental Research Center, Florida International University, Miami, FL 33199 (United States); Yang, Guidi [College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002 (China); Lagos, Leonel [Applied Research Center, Florida International University, Miami, FL 33199 (United States); Yin, Yongguang [State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China); Gu, Baohua [Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge TN 37831 (United States); Jiang, Guibin [State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China); Cai, Yong, E-mail: cai@fiu.edu [Department of Chemistry and Biochemistry, Florida International University, Miami, FL 33199 (United States); Southeast Environmental Research Center, Florida International University, Miami, FL 33199 (United States)

    2016-11-05

    Highlights: • Develop a new method to study Hg re-adsorption in cinnabar. • Both isotope dilution and tracer techniques were adopted. • The presence of O{sub 2} can significantly enhance the dissolution of cinnabar. • Prove the necessity of including re-adsorption in estimating cinnabar dissolution. - Abstract: Cinnabar dissolution is an important factor controlling mercury (Hg) cycling. Recent studies have suggested the co-occurrence of re-adsorption of the released Hg during the course of cinnabar dissolution. However, there is a lack of feasible techniques that can quantitatively assess the amount of Hg re-adsorbed on cinnabar when investigating cinnabar dissolution. In this study, a new method, based on isotope tracing and dilution techniques, was developed to study the role of Hg re-adsorption in cinnabar dissolution. The developed method includes two key components: (1) accurate measurement of both released and spiked Hg in aqueous phase and (2) estimation of re-adsorbed Hg on cinnabar surface via the reduction in spiked {sup 202}Hg{sup 2+}. By adopting the developed method, it was found that the released Hg for trials purged with oxygen could reach several hundred μg L{sup −1}, while no significant cinnabar dissolution was detected under anaerobic condition. Cinnabar dissolution rate when considering Hg re-adsorption was approximately 2 times the value calculated solely with the Hg detected in the aqueous phase. These results suggest that ignoring the Hg re-adsorption process can significantly underestimate the importance of cinnabar dissolution, highlighting the necessity of applying the developed method in future cinnabar dissolution studies.

  11. Identifying Seasonal Groundwater Recharge Using Environmental Stable Isotopes

    Directory of Open Access Journals (Sweden)

    Hsin-Fu Yeh

    2014-09-01

    Full Text Available In this study, the stable isotope values of oxygen and hydrogen were used to identify the seasonal contribution ratios of precipitation to groundwater recharge in the Hualien River basin of eastern Taiwan. The differences and correlations of isotopes in various water bodies were examined to evaluate the groundwater recharge sources for the Hualian River basin and the interrelations between groundwater and surface water. Proportions of recharge sources were calculated based on the results of the mass balance analysis of the isotope composition of hydrogen and oxygen in the basin. Mountain river water accounted for 83% and plain rainfall accounted for 17% of the groundwater recharge in the Huanlian River basin. Using the mean d-values, a comparison of d-values of precipitation and groundwater indicates the groundwater consists of 75.5% wet seasonal sources and 24.5% dry seasonal sources, representing a distinct seasonal variation of groundwater recharge in the study area. Comparisons between hydrogen and oxygen isotopes in rainwater showed that differences in the amount of rainfall resulted in depleted oxygen and hydrogen isotopes for precipitation in wet seasons as compared to dry seasons. The river water contained more depleted hydrogen and oxygen isotopes than was the case for precipitation, implying that the river water mainly came from the upstream catchment. In addition, the hydrogen and oxygen isotopes in the groundwater slightly deviated from the hydrogen and oxygen isotopic meteoric water line in Huanlian. Therefore, the groundwater in this basin might be a mixture of river water and precipitation, resulting in the effect of the river water recharge being greater than that of rainfall infiltration.

  12. Water vapor stable isotope observations from tropical Australia

    KAUST Repository

    Parkes, Stephen

    2015-04-01

    The response of the tropical hydrological cycle to anthropogenically induced changes in radiative forcing is one of the largest discrepancies between climate models. Paleoclimate archives of the stable isotopic composition of precipitation in the tropics indicate a relationship with precipitation amount that could be exploited to study past hydroclimate and improve our knowledge of how this region responds to changes in climate forcing. Recently modelling studies of convective parameterizations fitted with water isotopes and remote sensing of water vapor isotopes in the tropics have illustrated uncertainty in the assumed relationship with rainfall amount. Therefore there is a need to collect water isotope data in the tropics that can be used to evaluate these models and help identify the relationships between the isotopic composition of meteoric waters and rainfall intensity. However, data in this region is almost non-existent. Here we present in-situ water vapor isotopic measurements and the HDO retrievals from the co-located Total Column Carbon Observing Network (TCCON) site at Darwin in Tropical Australia. The Darwin site is interestingly placed within the tropical western pacific region and is impacted upon by a clear monsoonal climate, and key climate cycles including ENSO and Madden Julian Oscillations. The analysis of the data illustrated relationships between water vapor isotopes and humidity which demonstrated the role of precipitation processes in the wet season and air mass mixing during the dry season. Further the wet season observations show complex relationships between humidity and isotopes. A simple Rayleigh distillation model was not obeyed, instead the importance of rainfall re-evaporation in generating the highly depleted signatures was demonstrated. These data potentially provide a useful tool for evaluating model parameterizations in monsoonal regions as they demonstrate relationships with precipitation processes that cannot be observed with

  13. Tracing anthropogenic thallium in soil using stable isotope compositions.

    Science.gov (United States)

    Kersten, Michael; Xiao, Tangfu; Kreissig, Katharina; Brett, Alex; Coles, Barry J; Rehkämper, Mark

    2014-08-19

    Thallium stable isotope data are used in this study, for the first time, to apportion Tl contamination in soils. In the late 1970s, a cement plant near Lengerich, Germany, emitted cement kiln dust (CKD) with high Tl contents, due to cocombustion of Tl-enriched pyrite roasting waste. Locally contaminated soil profiles were obtained down to 1 m depth and the samples are in accord with a binary mixing relationship in a diagram of Tl isotope compositions (expressed as ε(205)Tl, the deviation of the (205)Tl/(203)Tl ratio of a sample from the NIST SRM 997 Tl isotope standard in parts per 10(4)) versus 1/[Tl]. The inferred mixing endmembers are the geogenic background, as defined by isotopically light soils at depth (ε(205)Tl ≈ -4), and the Tl emissions, which produce Tl-enriched topsoils with ε(205)Tl as high as ±0. The latter interpretation is supported by analyses of the CKD, which is also characterized by ε(205)Tl ≈ ± 0, and the same ε(205)Tl value was found for a pyrite from the deposit that produced the cocombusted pyrite roasting waste. Additional measurements for samples from a locality in China, with outcrops of Tl sulfide mineralization and associated high natural Tl backgrounds, reveal significant isotope fractionation between soils (ε(205)Tl ≈ +0.4) and locally grown green cabbage (ε(205)Tl between -2.5 and -5.4). This demonstrates that biological isotope fractionation cannot explain the isotopically heavy Tl in the Lengerich topsoils and the latter are therefore clearly due to anthropogenic Tl emissions from cement processing. Our results thus establish that isotopic data can reinforce receptor modeling for the toxic trace metal Tl.

  14. Stable isotopic composition of bottled mineral waters from Romania

    Science.gov (United States)

    Bădăluţă, Carmen; Nagavciuc, Viorica; Perșoiu, Aurel

    2015-04-01

    Romania has a high potential of mineral waters resources, featuring one of the largest mineral resources at European and global level. In the last decade, due to increased in consumption of bottled water, numerous brands have appeared on the market, with equally numerous and variable sources of provenance. In this study we have analyzed the isotopic composition of bottled mineral waters from Romania in order to determine their source and authenticity. We have analysed 32 carbonated and 24 non-carbonated mineral waters from Romania. and the results were analysed in comparison with stable isotope data from precipitation and river waters. Generally, the isotopic values of the mineral waters follow those in precipitation; however, differences occur in former volcanic regions (due to deep circulation of meteoric waters and increased exchange with host rock and volcanic CO2), as well as in mountainous regions, where high-altitude recharge occurs.

  15. Hitting the moving target: modelling ontogenetic shifts with stable isotopes reveals the importance of isotopic turnover.

    Science.gov (United States)

    Hertz, Eric; Trudel, Marc; El-Sabaawi, Rana; Tucker, Strahan; Dower, John F; Beacham, Terry D; Edwards, Andrew M; Mazumder, Asit

    2016-05-01

    Ontogenetic niche shifts are widely prevalent in nature and are important in shaping the structure and dynamics of ecosystems. Stable isotope analysis is a powerful tool to assess these shifts, with δ(15) N providing a measure of trophic level and δ(13) C a measure of energy source. Previous applications of stable isotopes to study ontogenetic niche shifts have not considered the appreciable time lag between diet and consumer tissue associated with isotopic turnover. These time lags introduce significant complexity into field studies of ontogenetic niche shifts. Juvenile Chinook salmon (Oncorhynchus tshawytscha) migrate from freshwater to marine ecosystems and shift their diet from feeding primarily on invertebrates to feeding primarily on fish. This dual ontogenetic habitat and diet shift, in addition to the long time lag associated with isotopic turnover, suggests that there is potential for a disconnect between the prey sources that juvenile salmon are consuming, and the inferred prey sources from stable isotopes. We developed a model that considered ontogenetic niche shifts and time lags associated with isotopic turnover, and compared this 'ontogeny' model to one that considered only isotopic turnover. We used a Bayesian framework to explicitly account for parameter uncertainty. Data showed overwhelming support for the ontogeny model relative to the isotopic turnover model. Estimated variables from best model fits indicate that the ontogeny model predicts a much greater reliance on fish prey than does the stomach content data. Overall, we found that this method of quantifying ontogenetic niche shifts effectively accounted for both isotopic turnover and ontogenetic diet shifts; a finding that could be widely applicable to a variety of systems.

  16. Stable isotopes in soil and water studies; Isotopes stables dans l`eau et le sol

    Energy Technology Data Exchange (ETDEWEB)

    Allison, G.B. [Division of Water Resources, Adelaide Laboratory, PMB2, South (Australia)

    1998-12-31

    The composition of oxygen-18 and deuterium in soil waters varies mainly as a result of changes in the isotopic composition of rainfall and the processes of evaporation. This paper gives examples of how the isotope profiles which develop in the unsaturated zone can give information about recharge fluxes and mechanisms, evaporation from groundwater systems in arid areas and the zones in soil profiles from which plants use water. J. Ch. Fontes and his colleagues have been leaders in the application of these techniques to the measurement of evaporation from deep water tables in arid areas. (author) 29 refs.

  17. Stable Isotopes of Ice: the Legacy of Willi Dansgaard

    Science.gov (United States)

    White, J. W. C.; Johnson, S. J.

    2012-04-01

    Stable isotope ratios of ice, D/H and 18O/16O are one of the key climate indicators measured in ice cores. These isotope ratios vary with temperature, a relationship based on physical principles backed up by many observations. The combination of these isotope ratios, expressed as the difference between the delta values with δ18O scaled by a factor of eight, is called the deuterium excess (d=δD-8*δ18O). This parameter varies primarily as a function of the conditions of evaporation of the parent moisture for snow, yielding a signal of ocean conditions measured in the ice. In his classic 1964 paper in Tellus, Willi Dansgaard laid out the theoretical and observational basis for using stable isotope ratios in ice cores as paleo-environmental tools. This paper, cited over 2,200 times, and written nearly 50 years ago, is one of the key foundational papers in paleoclimatology, and remains a must read for any student of stable isotope geochemistry. In this talk we will explore Dansgaard's legacy of ice core climatology, with a focus on his pioneering work in using the full temporal resolution of ice cores in Greenland to explore climate change on time scales of years to decades. While Dansgaard began his career applying a clever technique to a novel medium with the goal of simply trying to understand how our planet functions, he early on understood the power of ice cores to inform us about human impacts on the climate system, as well as the power of ice cores to tell us about natural climate variability on time scales of human interest and impact. Dansgaard's body of work is one of the solid pillars on which modern paleoclimatology stands, and continues to inform us today about modern anthropogenic climate change.

  18. Stable strontium isotopic ratios from archaeological organic remains from the Thorsberg peat bog

    DEFF Research Database (Denmark)

    Nosch, Marie-Louise Bech; von Carnap-Bornheim, Claus; Grupe, Gisela;

    2007-01-01

    Pilot study analysing stable strontium isotopic ratios from Iron Age textile and leather finds from the Thorsberg peat bog.......Pilot study analysing stable strontium isotopic ratios from Iron Age textile and leather finds from the Thorsberg peat bog....

  19. Fractionation of Stable Isotopes in Atmospheric Aerosol Reactions

    DEFF Research Database (Denmark)

    Meusinger, Carl

    -pinene - an abundant precursor to biogenic aerosol 3. Oxidation of SO2 to sulfuric acid - one of the key species in aerosol formation Laboratory experiments were designed and conducted as part of this thesis to investigate these processes. In addition, advanced data treatment and chemical modeling were performed...... reactions and undergo complex chemical and physical changes during their lifetimes. In order to assess processes that form and alter aerosols, information provided by stable isotopes can be used to help constrain estimates on the strength of aerosol sources and sinks. This thesis studies (mass......-independent) fractionation processes of stable isotopes of C, N, O and S in order to investigate three different systems related to aerosols: 1. Post-depositional processes of nitrate in snow that obscure nitrate ice core records 2. Formation and aging of secondary organic aerosol generated by ozonolysis of X...

  20. Development of stable isotope separation technology for radioisotope production

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Do Young; Kim, Cheol Jung; Park, Kyung Bae [and others

    2003-05-01

    The ultimate goal of this project is to construct the domestic production system of stable isotopes O-18 and Tl-203 used as target materials in accelerator for the production of medical radioisotopes F-18 and Tl-201, respectively. In order to achieve this goal, diode laser spectroscopic analytical system was constructed and automatic measurement computer software for the direct analysis of H{sub 2}{sup 16}O/H{sub 2}{sup 18}O ratio were developed. Distillation process, laser process, and membrane diffusion process were analyzed for the evaluation of O-18 production. And electromagnetic process, plasma process, and laser process were analyzed for the evaluation of Tl-203 production. UV laser system, IR laser system, and detailed system Tl-203 production were designed. Finally, current and future worldwide demand/supply of stable isotopes O-18 and Tl-203 were estimated.

  1. Neutron inelastic scattering measurements on the stable isotopes of titanium

    Science.gov (United States)

    Olacel, A.; Belloni, F.; Borcea, C.; Boromiza, M.; Dessagne, P.; Henning, G.; Kerveno, M.; Negret, A.; Nyman, M.; Pirovano, E.; Plompen, A. J. M.

    2017-07-01

    The results of a neutron inelastic scattering experiment performed at the Geel Electron Linear Accelerator pulsed white neutron source of the European Commission Joint Research Centre are reported. The neutrons with energies up to 18 MeV interacted with a natTi sample and the γ rays resulting from inelastic scattering reactions on the stable isotopes were detected using the Gamma Array for Inelastic Neutron Scattering (GAINS) spectrometer. We were able to measure the γ -production cross sections for 21 transitions in the five stable Ti isotopes. From these, the level cross sections and the total inelastic cross sections were determined. Our experimental results are compared with theoretical calculations performed using the talys 1.8 code, evaluated nuclear data libraries, and also with previously reported results.

  2. Diets of introduced predators using stable isotopes and stomach contents

    Science.gov (United States)

    Meckstroth, A.M.; Miles, A.K.; Chandra, S.

    2007-01-01

    In a study of predation on ground-nesting birds at South San Francisco Bay (South Bay), California, USA, we analyzed stomach contents and stable isotopes of carbon and nitrogen to identify commonly consumed prey. We obtained the stomach contents from 206 nonnative red foxes (Vulpes vulpes regalis) collected in the South Bay area and Monterey County during 1995-2001 and from 68 feral cats (Felis silvestris) from the South Bay area during 2001-2002. We determined prey identity, biomass, and frequency, described seasonal diet trends, and derived an Index of Relative Importance. Avian species were the most frequent prey we found in the stomachs of red foxes from South Bay (61%), whereas small rodents were most frequent for red foxes from Monterey County (62%). Small rodents were the most frequent prey we found in feral cats (63%). Carbon and nitrogen isotopic signatures for foxes supported stomach content findings. However, isotope results indicated that cats received a majority of their energy from a source other than rodents and outside the natural system, which differed from the stomach content analysis. We demonstrated the utility of both stable isotope and stomach content analyses to establish a more complete understanding of predators' diets. This information aids natural resource managers in planning and evaluating future predator-removal programs and increases our understanding of the impacts of nonnative foxes and cats on native species.

  3. Stable isotope reference materials at the IAEA - the latest developments.

    Science.gov (United States)

    Assonov, Sergey; Gröning, Manfred; Fajgelj, Ales

    2017-04-01

    During the last few years the IAEA has performed works in several directions as following: • Released IAEA-603 (replacement of NBS19) - this is primary Stable isotope Reference Material (RM) used for the VPDB 13C and 18O scale realisation, • Released 6 isotopically enriched (in 2H and 18O) waters, • Performed careful monitoring of LVEC' property delta-13C value. This resulted in understanding that LSVEC needs a replacement. • Taken part in characterisation of 18 new organic CRMs (together with Indiana University and USGS) • Creating collaborations with metrology institutes - focus on metrological aspects of RMs, • As a spin-off of the Technical Meeting on Stable Isotope Reference Materials (IAEA, Vienna 2014) the European EMPIR project aimed to create infrastructure to produce gas mixtures characterised in CO2 isotope composition has been established (NPL, UK and other metrological institutes). The presentation will give details of these works and overview of the current status of RMs production and development.

  4. Paleoclimate and Amerindians: evidence from stable isotopes and atmospheric circulation.

    Science.gov (United States)

    Lovvorn, M B; Frison, G C; Tieszen, L L

    2001-02-27

    Two Amerindian demographic shifts are attributed to climate change in the northwest plains of North America: at approximately 11,000 calendar years before present (yr BP), Amerindian culture apparently split into foothills-mountains vs. plains biomes; and from 8,000-5,000 yr BP, scarce archaeological sites on the open plains suggest emigration during xeric "Altithermal" conditions. We reconstructed paleoclimates from stable isotopes in prehistoric bison bone and relations between weather and fractions of C(4) plants in forage. Further, we developed a climate-change model that synthesized stable isotope, existing qualitative evidence (e.g., palynological, erosional), and global climate mechanisms affecting this midlatitude region. Our isotope data indicate significant warming from approximately 12,400 to 11,900 yr BP, supporting climate-driven cultural separation. However, isotope evidence of apparently wet, warm conditions at 7,300 yr BP refutes emigration to avoid xeric conditions. Scarcity of archaeological sites is best explained by rapid climate fluctuations after catastrophic draining of the Laurentide Lakes, which disrupted North Atlantic Deep Water production and subsequently altered monsoonal inputs to the open plains.

  5. Development of Laser Application Technology for Stable Isotope Production

    Energy Technology Data Exchange (ETDEWEB)

    Jeong, Do Young; Ko, Kwang Hoon; Kwon, Duck Hee [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)] (and others)

    2007-04-15

    Tl-203 is used as a source material to produce Tl-201 radioisotope which is produced in a cyclotron by irradiating the enriched Tl-203 target. Tl-201 is a radiopharmaceutical for SPECT (single photon emission computerized tomography) to diagnose heart diseases and tumors. This Project aim to develop laser application technology to product stable isotopes such as Tl-203, Yb-168, and Yb-176. For this, photoion extraction device, atomic beam generator, dye lasers, and high power IR lasers are developed.

  6. Stable isotope patterns in micronekton from the Mozambique Channel

    OpenAIRE

    2014-01-01

    We measured the stable carbon (delta C-13) and nitrogen (delta N-15) isotopic composition of tissues of micronektonic organisms (fishes, squids, crustaceans and gelatinous organisms) collected in the Mozambique Channel during two scientific cruises in 2008 and 2009. The oceanic circulation in the Mozambique Channel is dominated by mesoscale cyclonic and anticyclonic eddies which play a key role in biological processes of less-productive deep-sea ecosystems. We investigated the potential impac...

  7. Stable isotope patterns in micronekton from the Mozambique Channel

    OpenAIRE

    2014-01-01

    International audience; We measured the stable carbon (δ13C) and nitrogen (δ15N) isotopic composition of tissues of micronektonic organisms (fishes, squids, crustaceans and gelatinous organisms) collected in the Mozambique Channel during two scientific cruises in 2008 and 2009. The oceanic circulation in the Mozambique Channel is dominated by mesoscale cyclonic and anticyclonic eddies which play a key role in biological processes of less-productive deep-sea ecosystems. We investigated the pot...

  8. Divergence of stable isotopes in tap water across China

    Science.gov (United States)

    Zhao, Sihan; Hu, Hongchang; Tian, Fuqiang; Tie, Qiang; Wang, Lixin; Liu, Yaling; Shi, Chunxiang

    2017-03-01

    Stable isotopes in water (e.g., δ2H and δ18O) are important indicators of hydrological and ecological patterns and processes. Tap water can reflect integrated features of regional hydrological processes and human activities. China is a large country with significant meteorological and geographical variations. This report presents the first national-scale survey of Stable Isotopes in Tap Water (SITW) across China. 780 tap water samples have been collected from 95 cities across China from December 2014 to December 2015. (1) Results yielded the Tap Water Line in China is δ2H = 7.72 δ18O + 6.57 (r2 = 0.95). (2) SITW spatial distribution presents typical “continental effect”. (3) SITW seasonal variations indicate clearly regional patterns but no trends at the national level. (4) SITW can be correlated in some parts with geographic or meteorological factors. This work presents the first SITW map in China, which sets up a benchmark for further stable isotopes research across China. This is a critical step toward monitoring and investigating water resources in climate-sensitive regions, so the human-hydrological system. These findings could be used in the future to establish water management strategies at a national or regional scale.

  9. An evaluation of teeth of ringed seals (Phoca hispida) from Greenland as a matrix to monitor spatial and temporal trends of mercury and stable isotopes.

    OpenAIRE

    Aubail, Aurore; Dietz, Rune; Rigét, Frank; Simon-Bouhet, Benoit; Caurant, Florence

    2010-01-01

    International audience; Total mercury (Hg) concentrations were measured in teeth of ringed seals from Qeqertarsuaq, central West Greenland (1982 to 2006) and Ittoqqortoormiit, central East Greenland (1986 to 2006). Stable isotopic ratios of carbon (13C/12C) and nitrogen (15N/14N) were determined as well to provide insights into diet variations between regions or through time. Mercury concentrations decreased the first years of life of the animals suggesting that Hg had been transferred from t...

  10. Estimation of evapotranspiration rate in irrigated lands using stable isotopes

    Science.gov (United States)

    Umirzakov, Gulomjon; Windhorst, David; Forkutsa, Irina; Brauer, Lutz; Frede, Hans-Georg

    2013-04-01

    Agriculture in the Aral Sea basin is the main consumer of water resources and due to the current agricultural management practices inefficient water usage causes huge losses of freshwater resources. There is huge potential to save water resources in order to reach a more efficient water use in irrigated areas. Therefore, research is required to reveal the mechanisms of hydrological fluxes in irrigated areas. This paper focuses on estimation of evapotranspiration which is one of the crucial components in the water balance of irrigated lands. Our main objective is to estimate the rate of evapotranspiration on irrigated lands and partitioning of evaporation into transpiration using stable isotopes measurements. Experiments has done in 2 different soil types (sandy and sandy loam) irrigated areas in Ferghana Valley (Uzbekistan). Soil samples were collected during the vegetation period. The soil water from these samples was extracted via a cryogenic extraction method and analyzed for the isotopic ratio of the water isotopes (2H and 18O) based on a laser spectroscopy method (DLT 100, Los Gatos USA). Evapotranspiration rates were estimated with Isotope Mass Balance method. The results of evapotranspiration obtained using isotope mass balance method is compared with the results of Catchment Modeling Framework -1D model results which has done in the same area and the same time.

  11. Stable isotopes differentiate bottlenose dolphins off west-central Florida

    Science.gov (United States)

    Barros, Nélio B.; Ostrom, P. H.; Stricker, Craig A.; Wells, R.S.

    2010-01-01

    Distinguishing discrete population units among continuously distributed coastal small cetaceans is challenging and crucial to conservation. We evaluated the utility of stable isotopes in assessing group membership in bottlenose dolphins (Tursiops truncatus) off west-central Florida by analyzing carbon, nitrogen, and sulfur isotope values (δ13C, δ15N, and δ34S) of tooth collagen from stranded dolphins. Individuals derived from three putative general population units: Sarasota Bay (SB), nearshore Gulf of Mexico (GULF), and offshore waters (OFF). Animals of known history (SB) served to ground truth the approach against animals of unknown history from the Gulf of Mexico (GULF, OFF). Dolphin groups differed significantly for each isotope. Average δ13C values from SB dolphins (−10.6‰) utilizing sea grass ecosystems differed from those of GULF (−11.9‰) and OFF (−11.9‰). Average δ15N values of GULF (12.7‰) and OFF (13.2‰) were higher than those of SB dolphins (11.9‰), consistent with differences in prey trophic levels. δ34S values showed definitive differences among SB (7.1‰), GULF (11.3‰), and OFF (16.5‰) dolphins. This is the first application of isotopes to population assignment of bottlenose dolphins in the Gulf of Mexico and results suggest that isotopes may provide a powerful tool in the conservation of small cetaceans.

  12. Production of stable isotopes utilizing the plasma separation process

    Science.gov (United States)

    Bigelow, T. S.; Tarallo, F. J.; Stevenson, N. R.

    2005-12-01

    A plasma separation process (PSP) is being operated at Theragenics Corporation's®, Oak Ridge, TN, facility for the enrichment of stable isotopes. The PSP utilizes ion cyclotron mass discrimination to separate isotopes on a relatively large scale. With a few exceptions, nearly any metallic element could be processed with PSP. Output isotope enrichment factor depends on natural abundance and mass separation and can be fairly high in some cases. The Theragenics™ PSP facility is believed to be the only such process currently in operation. This system was developed and formerly operated under the US Department of Energy Advanced Isotope Separation program. Theragenics™ also has a laboratory at the PSP site capable of harvesting the isotopes from the process and a mass spectrometer system for analyzing enrichment and product purity. Since becoming operational in 2002, Theragenics™ has utilized the PSP to separate isotopes of several elements including: dysprosium, erbium, gadolinium, molybdenum and nickel. Currently, Theragenics™ is using the PSP for the separation of 102Pd, which is used as precursor for the production of 103Pd. The 103Pd radioisotope is the active ingredient in TheraSeed®, which is used in the treatment of early stage prostate cancer and being investigated for other medical applications. New industrial, medical and research applications are being investigated for isotopes that can be enriched on the PSP. Pre-enrichment of accelerator or reactor targets offers improved radioisotope production. Theragenics operates 14 cyclotrons for proton activation and has access to HFIR at ORNL for neutron activation of radioisotopes.

  13. Realistic Fasting Does Not Affect Stable Isotope Levels of a Metabolically Efficient Salamander

    Science.gov (United States)

    Stable isotopes are commonly used to examine various aspects of animal ecology. The use of stable isotopes generally proceeds under the implicit assumption that resource use is the only factor driving variation in stable isotope levels; however, a wealth of studies demonstrate a...

  14. Production of yeastolates for uniform stable isotope labelling in eukaryotic cell culture.

    NARCIS (Netherlands)

    Egorova-Zachernyuk, T.A.; Bosman, G.J.C.G.M.; Pistorius, A.M.A.; Grip, W.J. de

    2009-01-01

    Preparation of stable isotope-labelled yeastolates opens up ways to establish more cost-effective stable isotope labelling of biomolecules in insect and mammalian cell lines and hence to employ higher eukaryotic cell lines for stable isotope labelling of complex recombinant proteins. Therefore, we e

  15. Climatic signals in multiple highly resolved stable isotope records from Greenland

    DEFF Research Database (Denmark)

    Vinther, Bo Møllesøe; Dahl-Jensen, Dorthe; Johnsen, Sigfus Johann

    2010-01-01

    are found to correspond better with winter stable isotope data than with summer or annual average stable isotope data it is suggested that a strong local Greenland temperature signal can be extracted from the winter stable isotope data even on centennial to millennial time scales. Udgivelsesdato: Feb....

  16. Realistic Fasting Does Not Affect Stable Isotope Levels of a Metabolically Efficient Salamander

    Science.gov (United States)

    Stable isotopes are commonly used to examine various aspects of animal ecology. The use of stable isotopes generally proceeds under the implicit assumption that resource use is the only factor driving variation in stable isotope levels; however, a wealth of studies demonstrate a...

  17. Enantioselective stable isotope analysis (ESIA) of polar Herbicides

    Science.gov (United States)

    Maier, Michael; Qiu, Shiran; Elsner, Martin

    2013-04-01

    The complexity of aquatic systems makes it challenging to assess the environmental fate of chiral micropolutants. As an example, chiral herbicides are frequently detected in the environment (Buser and Muller, 1998); however, hydrological data is needed to determine their degradability from concentration measurements. Otherwise declining concentrations cannot unequivocally be attributed to degradation, but could also be caused by dilution effects. In contrast, isotope ratios or enantiomeric ratios are elegant alternatives that are independent of dilution and can even deliver insights into reaction mechanisms. To combine the advantages of both approaches we developed an enatioselective stable isotope analysis (ESIA) method to investigate the fate of the chiral herbicides 4-CPP ((RS)-2-(4-chlorophenoxy)-propionic acid), mecoprop (2-(4-Chloro-2-methylphenoxy)-propionic acid) and dichlorprop (2-(2,4-Dichlorophenoxy)-propionic acid). After testing the applicable concentration range of the method, enantioselective isotope fractionation was investigated by microbial degradation using dichlorprop as a model compound. The method uses enantioselective gas-chromatography (GC) to separate enantiomers. Subsequently samples are combusted online to CO2 and carbon isotope ratios are determined for each enantiomer by isotope-ratio-mass-spectrometry (IRMS). Because the analytes contain a polar carboxyl-group, samples were derivatised prior to GC-IRMS analysis with methanolic BF3 solution. Precise carbon isotope analysis (2σ ≤0.5‰) was achieved with a high sensitivity of ≥ 7 ng C that is needed on column for one analysis. Microbial degradation of the model compound dichlorprop was conducted with Delftia acidovorans MC1 and pronounced enantiomer fractionation, but no isotope fractionation was detected. The absence of isotope fractionation can be explained by two scenarios: either the degrading enzyme has no isotopic preference, or another step in the reaction without an isotopic

  18. Historical Records of Mercury Stable Isotopes in Sediments of Tibetan Lakes

    Science.gov (United States)

    Yin, Runsheng; Feng, Xinbin; Hurley, James P.; Krabbenhoft, David P.; Lepak, Ryan F.; Kang, Shichang; Yang, Handong; Li, Xiangdong

    2016-01-01

    The Tibetan Plateau (TP), known as the “Third Pole”, is a critical zone for atmospheric mercury (Hg) deposition. Increasing anthropogenic activities in the globe leads to environmental changes, which may affect the loading, transport and deposition of Hg in the environment. However, the deposition history and geochemical cycling of Hg in the TP is still uncertain. Our records of Hg and Hg isotopes in sediment profiles of the two largest lakes in the TP, Lake Qinghai and Nam Co, show increased Hg influx since last century, with the maximum Hg influx enrichment ratios of 5.4 and 3.5 in Lake Qinghai and Nam Co, respectively. Shifts in negative δ 202Hg in Lake Qinghai (−4.55 to −3.15‰) and Nam Co (−5.04 to −2.16‰) indicate increased atmospheric Hg deposition through rainfall, vegetation and runoff of soils. Mass independent fractionation of both even-Hg (∆ 200Hg: +0.05 to +0.10‰) and odd-Hg (∆ 199Hg: +0.12 to +0.31‰) isotopes were observed. Positive Δ 200Hg suggest high proportion of precipitation-derived Hg in the TP, whereas the positive Δ 199Hg results from Hg(II) photo-reduction. Both lakes show increasing Δ 199Hg since the 1900 s, and we conclude that with the decrease of ice duration, Hg(II) photo-reduction may have been accelerated in these TP lakes. PMID:26996936

  19. Assessing the Amazon Basin Circulation with Stable Water Isotopes

    Science.gov (United States)

    McGuffie, K.; Henderson-Sellers, A.

    2004-05-01

    The isotopic abundances of Oxygen-18 (δ 18O) and Deuterium (δ D) over the Amazon are used to constrain simulations of the water cycle in this, the largest river basin in the world. Tracking the two stable but rare isotopes of water (1HD16O and 1H218O) makes it possible to trace Amazonian regional evaporative and condensation processes. This offers isotopic constraints on regional to global-scale atmospheric moisture budgets. Based on data in the Global Network on Isotopes in Precipitation (GNIP) database, we analyse the simulation of the land surface hydrology and water cycling. Temporal changes between 1965 and 2000 in stable water isotopic signatures in the Amazon have been used to evaluate global climate model (GCM) predictions revealing notable anomalies. For example, the differences in the wet season deuterium excess between Belem and Manaus are consistent with recent GCM simulations only if there has been a relative increase in evaporation from non-fractionating water sources over this period. Despite earlier predictions that land-use change signals would be found, late twentieth century data reveal no significant change in dry season isotopic characteristics. On the other hand, more recent isotopic data do show trends at stations in the Andes, where as much as 88% of the rainfall is thought to be derived from recycled moisture. At Izobamba the wet season depletions are enhanced (greater depletion) and the dry season ones decreased (less depletion). At Bogota only the wet months show statistically significant changes - also an enhancement. More depletion in the wet months is consistent with reductions in non-fractioning recycling such as through transpiration and in full re-evaporation of canopy-intercepted rainfall. These data might be linked to deforestation impacts. Results of GCM and simpler model simulations of the Amazon suggest that the recent stable isotope record is consistent with the predicted effects of forest removal, perhaps combined with

  20. Modeling stable isotope and organic carbon in hillslope stormflow

    Science.gov (United States)

    Dusek, Jaromir; Vogel, Tomas; Dohnal, Michal; Marx, Anne; Jankovec, Jakub; Sanda, Martin; Votrubova, Jana; Barth, Johannes A. C.; Cislerova, Milena

    2016-04-01

    Reliable prediction of water movement and fluxes of dissolved substances (such as stable isotopes and organic carbon) at both the hillslope and the catchment scales remains a challenge due to complex boundary conditions and soil spatial heterogeneity. In addition, microbially mediated transformations of dissolved organic carbon (DOC) are known to affect balance of DOC in soils, hence the transformations need to be included in a conceptual model of a DOC transport. So far, only few studies utilized stable isotope information in modeling and even fewer linked dissolved carbon fluxes to mixing and/or transport models. In this study, stormflow dynamics of oxygen-18 isotope and dissolved organic carbon was analyzed using a physically based modeling approach. One-dimensional dual-continuum vertical flow and transport model, based on Richards and advection-dispersion equations, was used to simulate the subsurface transport processes in a forest soil during several observed rainfall-runoff episodes. The transport of heat in the soil profile was described by conduction-advection equation. Water flow and transport of solutes and heat were assumed to take place in two mutually communicating porous domains, the soil matrix and the network of preferential pathways. The rate of microbial transformations of DOC was assumed to depend on soil water content and soil temperature. Oxygen-18 and dissolved organic carbon concentrations were observed in soil pore water, hillslope stormflow (collected in the experimental hillslope trench), and stream discharge (at the catchment outlet). The modeling was used to analyze the transformation of input solute signals into output hillslope signals observed in the trench stormflow. Signatures of oxygen-18 isotope in hillslope stormflow as well as isotope concentration in soil pore water were predicted reasonably well. Due to complex nature of microbial transformations, prediction of DOC rate and transport was associated with a high uncertainty.

  1. Stable isotope ratios in hair and teeth reflect biologic rhythms.

    Directory of Open Access Journals (Sweden)

    Otto Appenzeller

    Full Text Available Biologic rhythms give insight into normal physiology and disease. They can be used as biomarkers for neuronal degenerations. We present a diverse data set to show that hair and teeth contain an extended record of biologic rhythms, and that analysis of these tissues could yield signals of neurodegenerations. We examined hair from mummified humans from South America, extinct mammals and modern animals and people, both healthy and diseased, and teeth of hominins. We also monitored heart-rate variability, a measure of a biologic rhythm, in some living subjects and analyzed it using power spectra. The samples were examined to determine variations in stable isotope ratios along the length of the hair and across growth-lines of the enamel in teeth. We found recurring circa-annual periods of slow and fast rhythms in hydrogen isotope ratios in hair and carbon and oxygen isotope ratios in teeth. The power spectra contained slow and fast frequency power, matching, in terms of normalized frequency, the spectra of heart rate variability found in our living subjects. Analysis of the power spectra of hydrogen isotope ratios in hair from a patient with neurodegeneration revealed the same spectral features seen in the patient's heart-rate variability. Our study shows that spectral analysis of stable isotope ratios in readily available tissues such as hair could become a powerful diagnostic tool when effective treatments and neuroprotective drugs for neurodegenerative diseases become available. It also suggests that similar analyses of archaeological specimens could give insight into the physiology of ancient people and animals.

  2. Magnesium stable isotope ecology using mammal tooth enamel.

    Science.gov (United States)

    Martin, Jeremy E; Vance, Derek; Balter, Vincent

    2015-01-13

    Geochemical inferences on ancient diet using bone and enamel apatite rely mainly on carbon isotope ratios (δ(13)C) and to a lesser extent on strontium/calcium (Sr/Ca) and barium/calcium (Ba/Ca) elemental ratios. Recent developments in nontraditional stable isotopes provide an unprecedented opportunity to use additional paleodietary proxies to disentangle complex diets such as omnivory. Of particular relevance for paleodietary reconstruction are metals present in large quantity in bone and enamel apatite, providing that biologically mediated fractionation processes are constrained. Calcium isotope ratios (δ(44)Ca) meet these criteria but exhibit complex ecological patterning. Stable magnesium isotope ratios (δ(26)Mg) also meet these criteria but a comprehensive understanding of its variability awaits new isotopic data. Here, 11 extant mammal species of known ecology from a single locality in equatorial Africa were sampled for tooth enamel and, together with vegetation and feces, analyzed for δ(26)Mg, δ(13)C, Sr/Ca, and Ba/Ca ratios. The results demonstrate that δ(26)Mg incorporated in tooth enamel becomes heavier from strict herbivores to omnivores/faunivores. Using data from experimentally raised sheep, we suggest that this (26)Mg enrichment up the trophic chain is due to a (26)Mg enrichment in muscle relative to bone. Notably, it is possible to distinguish omnivores from herbivores, using δ(26)Mg coupled to Ba/Ca ratios. The potential effects of metabolic and dietary changes on the enamel δ(26)Mg composition remain to be explored but, in the future, multiproxy approaches would permit a substantial refinement of dietary behaviors or enable accurate trophic reconstruction despite specimen-limited sampling, as is often the case for fossil assemblages.

  3. The cycle of stable isotopes and tritium in water

    Energy Technology Data Exchange (ETDEWEB)

    Hunziker, J.C. [Inst. de Mineralogie, Lausanne (Switzerland)

    1998-12-31

    In hydrology, meteorology, climatology and environmental research natural hydrogen and oxygen isotopes of water increasingly replace radioactive or chemical tracers used in the past to track fluid paths through nature. This evolution has made possible through the network of precipitation data established since 1961 by the International Atomic Energy Agency (IAEA) in Vienna, that provides the necessary data base for the stable isotope interpretations. Evaporation and condensation and, at higher temperatures, water-rock interaction are the main mechanisms responsible for changing the isotopic ratio of water. These data provide insight into mean residence time of a water body and/or mean altitude of a catchment area. They also yield climatological information and allow the mass transfer quantification because of the temperature dependence of equilibrium fractionation. As the acquired isotopic ratio of a water is generally conserved during flow and percolation through geologic bodies, admixing of water showing different histories can easily be monitored, and when the end members are known or can be measured, mixtures can be calculated. Including isotopic measurements of certain anions, often dissolved in remarkable quantities in groundwaters e.g. nitrates, sulfates or phosphates, increases the reliability and the possibility to trace fluid paths through geologic bodies. In addition in environmental studies, oxygen, nitrogen and sulfur isotopes allow for source determination studies, again providing a possibility of quantification, which can not be obtained by any other method. Recent advances in the specific determination of organic molecules by means of GC-IRMS (gas-chromatography-isotope ratio mass spectrometry) has considerably enlarged our possibilities in detection and quantification of organic water pollution. These organic compounds (debris of macromolecules) increasingly deteriorate the quality of our drinking water; unfortunately they are normally not

  4. The quality control of fruit juices by using the stable isotope ratios and trace metal elements concentrations

    Science.gov (United States)

    Magdas, D. A.; Dehelean, A.; Puscas, R.; Cristea, G.; Tusa, F.; Voica, C.

    2012-02-01

    In the last years, a growing number of research articles detailing the use of natural abundance light stable isotopes variations and trace metal elements concentration as geographic "tracers" to determine the provenance of food have been published. These investigations exploit the systematic global variations of stable hydrogen, oxygen and carbon isotope ratios in (combination) relation with trace metal element concentrations. The trace metal elements content of plants and also their light stable isotopic ratios are mainly related to the geological and pedoclimatic characteristics of the site of growth. The interpretation of such analysis requires an important number of data for authentic natural juices regarding the same seasonal and regional origin, because the isotopic analysis parameters of fruit juices show remarkable variability depending on climatologically factors. In this work was mesured H, C, O stable isotope ratios and the concentrations of 16 elements (P, K, Mg, Na, Ca, Cu, Cr, Ni, Zn, Pb, Co, As, Cd, Mn, Fe and Hg) from 12 single strength juices. The natural variations that appear due to different environmental and climatic conditions are presented and discussed.

  5. Analytical techniques in biomedical stable isotope applications : (isotope ratio) mass spectrometry or infrared spectrometry?

    NARCIS (Netherlands)

    Stellaard, F; Elzinga, H

    2005-01-01

    An overview is presented of biomedical applications of stable isotopes in general, but mainly focused on the activities of the Center for Liver, Digestive and Metabolic Diseases of the University Medical Center Groningen. The aims of metabolic studies in the areas of glucose, fat, cholesterol and

  6. Evaluating mercury biomagnification in fish from a tropical marine environment using stable isotopes (delta13C and delta15N).

    Science.gov (United States)

    Al-Reasi, Hassan A; Ababneh, Fuad A; Lean, David R

    2007-08-01

    Concentrations of total mercury (T-Hg) and methylmercury (MeHg) were measured in zooplankton and 13 fish species from a coastal food web of the Gulf of Oman, an arm of the Arabian Sea between Oman and Iran. Stable isotope ratios (delta13C and delta15N) also were determined to track mercury biomagnification. The average concentration of T-Hg in zooplankton was 21 +/- 8.0 ng g(-1) with MeHg accounting 10% of T-Hg. Total mercury levels in fish species ranged from 3.0 ng g(-1) (Sardinella longiceps) to 760 ng g(-1) (Rhizoprionodon acutus) with relatively lower fraction of MeHg (72%) than that found in other studies. The average trophic difference (Deltadelta13C) between zooplankton and planktivorous fish (Selar crumenopthalmus, Rastrelliger kanagurta, and S. longiceps) was higher (3.4 per thousandth) than expected, suggesting that zooplankton may not be the main diet or direct carbon source for these fish species. However, further sampling would be required to compensate for temporal changes in zooplankton and the influence of their lipid content. Trophic position inferred by delta15N and and slopes of the regression equations (log10[T-Hg] = 0.13[delta15N] - 3.57 and log10[MeHg] = 0.14[delta15N] - 3.90) as estimates of biomagnification indicate that biomagnification of T-Hg and MeHg was lower in this tropical ocean compared to what has been observed in arctic and temperate ecosystems and tropical African lakes. The calculated daily intake of methylmercury in the diet of local people through fish consumption was well below the established World Health Organization (WHO) tolerable daily intake threshold for most of the fish species except Euthynnus affinis, Epinephelus epistictus, R. acutus, and Thunnus tonggol, illustrating safe consumption of the commonly consumed fish species.

  7. Magnesium stable isotope composition of Earth's upper mantle

    Science.gov (United States)

    Handler, Monica R.; Baker, Joel A.; Schiller, Martin; Bennett, Vickie C.; Yaxley, Gregory M.

    2009-05-01

    The mantle is Earth's largest reservoir of Mg containing > 99% of Earth's Mg inventory. However, no consensus exists on the stable Mg isotope composition of the Earth's mantle or how variable it is and, in particular, whether the mantle has the same stable Mg isotope composition as chondrite meteorites. We have determined the Mg isotope composition of olivine from 22 mantle peridotites from eastern Australia, west Antarctica, Jordan, Yemen and southwest Greenland by pseudo-high-resolution MC-ICP-MS on Mg purified to > 99%. The samples include fertile lherzolites, depleted harzburgites and dunites, cryptically metasomatised ('dry') peridotites and modally metasomatised apatite ± amphibole-bearing harzburgites and wehrlites. Olivine from these samples of early Archaean through to Permian lithospheric mantle have δ25Mg DSM-3 = - 0.22 to - 0.08‰. These data indicate the bulk upper mantle as represented by peridotite olivine is homogeneous within current analytical uncertainties (external reproducibility ≤ ± 0.07‰ [2 sd]). We find no systematic δ25Mg variations with location, lithospheric age, peridotite fertility, or degree or nature of mantle metasomatism. Although pyroxene may have slightly heavier δ25Mg than coexisting olivine, any fractionation between mantle pyroxene and olivine is also within current analytical uncertainties with a mean Δ25Mg pyr-ol = +0.06 ± 0.10‰ (2 sd; n = 5). Our average mantle olivine δ25Mg DSM-3 = - 0.14 ± 0.07‰ and δ26Mg DSM-3 = - 0.27 ± 0.14‰ (2 sd) are indistinguishable from the average of data previously reported for terrestrial basalts, confirming that basalts have stable Mg isotope compositions representative of the mantle. Olivine from five pallasite meteorites have δ25Mg DSM-3 = - 0.16 to - 0.11‰ that are identical to terrestrial olivine and indistinguishable from the average δ25Mg previously reported for chondrites. These data provide no evidence for measurable heterogeneity in the stable Mg isotope

  8. Isotope separation of the Yb-168 stable isotope for low energy gamma ray sources

    Energy Technology Data Exchange (ETDEWEB)

    Park, Hyun Min; Kwon, Duck Hee; Cha, Yong Ho; Lee, Ki Tae; Nam, Sung Mo; Yoo, Jaek Won; Han, Jae Min; Rhee, Yong Joo [Lab. of Quantum Optics, Korea Atomic Energy Research Institute, Taejeon (Korea, Republic of)

    2003-07-01

    We developed laser isotope separation technology of stable isotope of low melting point metals. Yb-168 can be effectively used in non-destructive testing (NDT) after it is transformed to Yb-168 by neutron irradiation in a nuclear reactor. For this application of Yb-168, the isotope purity of it should be enhanced to more than 15% from the natural abundance of 0.135%. Our isotope separation system consist of laser system, Yb vapor generating system, and photoionized particle extraction system. For the system, we developed a diode-pumped slid-state laser of high-repetition rate and 3-color dye lasers. Yb vapor was generated by heating solid Yb sample resistively. The photo-ion produced by resonance ionization were extracted by a devised extractor. We produced enriched Yb metal more than 20 mg with the abundance of 25.8% of Yb-168 in the Yb (NO{sub 3}){sub 3}.

  9. Stable isotope mass balance of lakes: a contemporary perspective

    Science.gov (United States)

    Gibson, J. J.; Birks, S. J.; Yi, Y.

    2016-01-01

    The theoretical basis for application of stable isotope mass balance of lakes is described for a range of climatic situations including low latitude, high latitude, high altitude, continental and coastal systems, as well as cases where the atmospheric boundary layer is significantly modified by the lake evaporation process. The effects of seasonality on isotopic offset between precipitation and atmospheric vapour and the slope of the local evaporation line are described. Atmospheric feedback and its role in labelling the isotopic composition of the Laurentian Great Lakes and tropical lakes is discussed. Several important considerations are suggested to improve parameterization of quantitative paleoclimatic reconstructions including use of assumptions that are appropriate for the climatic setting, for the atmospheric feedback situation, for salinity, and headwater setting. Potential for use of dual-isotopes to trace past changes in seasonality and input, and a dual-lake index method that can potentially be used to trace connectivity of lakes are presented. In cases where modern or paleo-evaporation systems may be under-defined there are inherent limitations in the degree of quantification that can be attained.

  10. Calcium kinetics with microgram stable isotope doses and saliva sampling

    Science.gov (United States)

    Smith, S. M.; Wastney, M. E.; Nyquist, L. E.; Shih, C. Y.; Wiesmann, H.; Nillen, J. L.; Lane, H. W.

    1996-01-01

    Studies of calcium kinetics require administration of tracer doses of calcium and subsequent repeated sampling of biological fluids. This study was designed to develop techniques that would allow estimation of calcium kinetics by using small (micrograms) doses of isotopes instead of the more common large (mg) doses to minimize tracer perturbation of the system and reduce cost, and to explore the use of saliva sampling as an alternative to blood sampling. Subjects received an oral dose (133 micrograms) of 43Ca and an i.v. dose (7.7 micrograms) of 46Ca. Isotopic enrichment in blood, urine, saliva and feces was well above thermal ionization mass spectrometry measurement precision up to 170 h after dosing. Fractional calcium absorptions determined from isotopic ratios in blood, urine and saliva were similar. Compartmental modeling revealed that kinetic parameters determined from serum or saliva data were similar, decreasing the necessity for blood samples. It is concluded from these results that calcium kinetics can be assessed with micrograms doses of stable isotopes, thereby reducing tracer costs and with saliva samples, thereby reducing the amount of blood needed.

  11. Thermal Neutron Capture onto the Stable Tungsten Isotopes

    Directory of Open Access Journals (Sweden)

    Nichols A.

    2012-02-01

    Full Text Available Thermal neutron-capture measurements of the stable tungsten isotopes have been carried out using the guided thermal-neutron beam at the Budapest Reactor. Prompt singles spectra were collected and analyzed using the HYPERMET γ-ray analysis software package for the compound tungsten systems 183W, 184W, and 187W, prepared from isotopically-enriched samples of 182W, 183W, and 186W, respectively. These new data provide both confirmation and new insights into the decay schemes and structure of the tungsten isotopes reported in the Evaluated Gamma-ray Activation File based upon previous elemental analysis. The experimental data have also been compared to Monte Carlo simulations of γ-ray emission following the thermal neutron-capture process using the statistical-decay code DICEBOX. Together, the experimental cross sections and modeledfeeding contribution from the quasi continuum, have been used to determine the total radiative thermal neutron-capture cross sections for the tungsten isotopes and provide improved decay-scheme information for the structural- and neutron-data libraries.

  12. Precipitation regime and stable isotopes at Dome Fuji, East Antarctica

    Science.gov (United States)

    Dittmann, Anna; Schlosser, Elisabeth; Masson-Delmotte, Valérie; Powers, Jordan G.; Manning, Kevin W.; Werner, Martin; Fujita, Koji

    2016-06-01

    A unique set of 1-year precipitation and stable water isotope measurements from the Japanese Antarctic station, Dome Fuji, has been used to study the impact of the synoptic situation and the precipitation origin on the isotopic composition of precipitation on the Antarctic Plateau. The Antarctic Mesoscale Prediction System (AMPS) archive data are used to analyse the synoptic situations that cause precipitation. These situations are investigated and divided into five categories. The most common weather situation during a precipitation event is an upper-level ridge that extends onto the Antarctic Plateau and causes strong northerly advection from the ocean. Most precipitation events are associated with an increase in temperature and wind speed, and a local maximum of δ18O. During the measurement period, 21 synoptically caused precipitation events caused 60 % of the total annual precipitation, whereas the remaining 40 % were predominantly attributed to diamond dust. By combining the synoptic analyses with 5-day back-trajectories, the moisture source regions for precipitation events were estimated. An average source region around a latitude of 55° S was found. The atmospheric conditions in the source region were used as initial conditions for running a Rayleigh-type isotopic model in order to reproduce the measured isotopic composition of fresh snow and to investigate the influence of the precipitation source region on the isotope ratios. The model represents the measured annual cycle of δ18O and the second-order isotopic parameter deuterium excess reasonably well, but yields on average too little fractionation along the transport/cooling path. While simulations with an isotopic general circulation model (GCM) (ECHAM5-wiso) for Dome Fuji are on average closer to the observations, this model cannot reproduce the annual cycle of deuterium excess. In the event-based analysis, no evidence of a correlation of the measured deuterium excess with the latitude of the

  13. Stable isotope paleoaltimetry and the evolution of landscapes and life

    Science.gov (United States)

    Mulch, Andreas

    2016-01-01

    Reconstructing topography of our planet not only advances our knowledge of the geodynamic processes that shape the Earth's surface; equally important it adds a key element towards understanding long-term continental moisture transport, atmospheric circulation and the distribution of biomes and biodiversity. Stable isotope paleoaltimetry exploits systematic decreases in the oxygen (δ18O) or hydrogen (δD) isotopic composition of precipitation along a mountain front when the interaction of topography and advected moist air masses induces orographic precipitation. These changes in δ18O or δD can be recovered from the geologic record and recent geochemical and modeling advances allow a broad range of proxy materials to be evaluated. Over the last 10 yr stable isotope paleoaltimetry has witnessed rapidly expanding research activities and has produced a broad array of fascinating tectonic and geomorphologic studies many of which have concentrated on determining the elevation history of continental plateau regions. These single-site studies have greatly expanded what used to be very sparse global paleoaltimetric data. The challenge now lies in disentangling the surface uplift component from the impact of climate change on δ18O and δD in precipitation. The robustness of stable isotope paleoaltimetry can be enhanced when high-elevation δ18O or δD data are referenced against low-elevation sites that track climate-modulated sea level δ18O or δD of precipitation through time (' δ- δ approach'). Analysis of central Andean paleosols documents that differences in δ18O of soil carbonate between the Subandean foreland and the Bolivian Altiplano are small between 11 and 7 Ma but rise rapidly to ca. 2.9‰ after 7 Ma, corroborating the magnitude of late Miocene change in δ18O on the Altiplano. Future advances in stable isotope paleoaltimetry will greatly benefit from addressing four key challenges: (1) Identifying topographically-induced changes in atmospheric

  14. An iron stable isotope comparison between human erythrocytes and plasma.

    Science.gov (United States)

    von Blanckenburg, Friedhelm; Oelze, Marcus; Schmid, Dietmar G; van Zuilen, Kirsten; Gschwind, Hans-Peter; Slade, Alan J; Stitah, Sylvie; Kaufmann, Daniel; Swart, Piet

    2014-11-01

    We present precise iron stable isotope ratios measured by multicollector-ICP mass spectrometry (MC-ICP-MS) of human red blood cells (erythrocytes) and blood plasma from 12 healthy male adults taken during a clinical study. The accurate determination of stable isotope ratios in plasma first required substantial method development work, as minor iron amounts in plasma had to be separated from a large organic matrix prior to mass-spectrometric analysis to avoid spectroscopic interferences and shifts in the mass spectrometer's mass-bias. The (56)Fe/(54)Fe ratio in erythrocytes, expressed as permil difference from the "IRMM-014" iron reference standard (δ(56/54)Fe), ranges from -3.1‰ to -2.2‰, a range typical for male Caucasian adults. The individual subject erythrocyte iron isotope composition can be regarded as uniform over the 21 days investigated, as variations (±0.059 to ±0.15‰) are mostly within the analytical precision of reference materials. In plasma, δ(56/54)Fe values measured in two different laboratories range from -3.0‰ to -2.0‰, and are on average 0.24‰ higher than those in erythrocytes. However, this difference is barely resolvable within one standard deviation of the differences (0.22‰). Taking into account the possible contamination due to hemolysis (iron concentrations are only 0.4 to 2 ppm in plasma compared to approx. 480 ppm in erythrocytes), we model the pure plasma δ(56/54)Fe to be on average 0.4‰ higher than that in erythrocytes. Hence, the plasma iron isotope signature lies between that of the liver and that of erythrocytes. This difference can be explained by redox processes involved during cycling of iron between transferrin and ferritin.

  15. Stable isotope separation; Separations physicochimiques d'isotopes stables realisations et etudes de petites productions

    Energy Technology Data Exchange (ETDEWEB)

    Botter, F.; Molinari, Ph.; Dirian, G. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1964-07-01

    Pure deuterium has been separated from gaseous mixtures of hydrogen and deuterium by band displacement chromatography, using columns of palladium on a support. The best results were obtained with columns of Pd on sintered {alpha} alumina. With a column of this type, of total capacity about 2 liters, a preparative apparatus of low dead volume has been built which produces 1 liter of pure D{sub 2} from a 50 p. 100 D{sub 2}, 50 p. 100 100 H{sub 2} mixture in about 12 minutes. As a first approximation chromatography is likened theoretically to counter current fractionation, neglecting superficial resistance to the exchange. and also longitudinal diffusions. The number of theoretical plates required necessary for a certain enrichment of the gas phase is determined graphically or by calculation, enabling comparisons to be made between the efficiencies of columns containing different amounts of palladium. Thermal Diffusion: For the separation of hydrogen isotopes a thermal diffusion installation, made of stainless steel and entirely tele-commanded has been constructed. The separation cascade is made up of two identical pairs of hot wire columns. Each pair can work separately or they may be connected by a thermosyphon. The temperature of the hot wire is kept at around 1000 deg C by direct current. With this installation, hydrogen samples with a deuterium content lower than o,5 ppm were obtained from a gas originally containing 32 ppm. It was thus possible to prepare tritium of 99,3 p. 100 concentration from gas with an initial content of 6 p. 100. For quantitative separation of xenon enriched five time in {sup 124}Xe by thermal diffusion, two identical cascades were constructed, each consisting of 5 columns, working in parallel and the two being connected by thermosyphon or by a capillary tube linked to a thermal gas oscillation. The central tungsten wire is heated to 1200 deg C. The columns are grouped like cluster of a heat exchanger, in shell of 30 cm diameter through

  16. Issues and opportunities in accelerator mass spectrometry for stable isotopes.

    Science.gov (United States)

    Matteson, Sam

    2008-01-01

    Accelerator mass spectrometry (AMS) has developed in the last 30 years many notable applications to the spectrometry of radioisotopes, particularly in radiocarbon dating. The instrumentation science of trace element AMS (TEAMS) that analyzes stable isotopes, also called Accelerator SIMS or MegaSIMS, while unique in many features, has also shared in many of these significant advances and has pushed TEAMS sensitivity to concentration levels surpassing many competing mass spectroscopic technologies. This review examines recent instrumentation developments, the capabilities of the new instrumentation and discernable trends for future development.

  17. Stable nitrogen isotopes in coastal macroalgae: geographic and anthropogenic variability

    OpenAIRE

    González-Viana, I. (Inés); Bode, A.

    2013-01-01

    Proyectos ANILE (CTM2009-08396 and CTM2010-08804-E) del Plan Nacional de I+D+i y RADIALES del Instituto Español de Oceanografía (IEO). I.G.V. recibió un contrato FPI del Ministerio de Economía y Competividad Growing human population add to the natural nitrogen loads to coastal waters. As the excess nitrogen is readily incorporated in new biomass anthropogenic and natural nitrogen sources may be traced by the measurement of stable nitrogen isotopes (δ15N). In this study δ15N was dete...

  18. Contribution of stable isotopes to the study of pharmacokinetics of magnesium salts; Apport des isotopes stables a l'etude de la pharmacocinetique de sels de magnesium

    Energy Technology Data Exchange (ETDEWEB)

    Benech, H

    1999-05-28

    The use of stable isotopes as labels is becoming an attractive tool for the study of magnesium behavior in humans. It has been used two stable isotopes of magnesium, {sup 25}Mg and {sup 26}Mg, to measure the absolute bioavailability of a pharmaceutical form of magnesium. (N.C.)

  19. Progress update on IUPAC Project 2009-046-2-200: Terminology and definition of quantities related to the isotope distribution in elements with more than two stable isotopes

    Science.gov (United States)

    Kaiser, Jan; Angert, Alon; Bergquist, Bridget; Brand, Willi; Ono, Shuhei; Röckmann, Thomas; Savarino, Joël

    2014-05-01

    The objective of IUPAC Project 2009-046-2-200 (http://www.iupac.org/web/ins/2009-046-2-200) is to define terminology and to identify the most suitable definitions of quantities that characterise the isotope distribution in elements with more than two stable isotopes, including so-called mass-independent fractionation, non-mass dependent fractionation, isotope anomaly, 17O excess, etc. Most atmospheric oxygen-bearing species show deviations in their triple oxygen isotope ratios from mass-dependent fractionation (MDF) relationships predicted by the theories of Urey, Bigeleisen and Mayer. Similar deviations have also been found in sulphur and other elements with more than two stables isotopes (e.g. Hg, Cd, Zn), often preserved in non-atmospheric reservoirs, including rocks, minerals, soils, ice and waters. Despite the ubiquity of this type of isotope anomaly, there has never been an attempt to clearly define the terminology and physical quantities used to measure these anomalies and the processes that lead to their formation. Terms like mass-independent fractionation, non-mass dependent fractionation, isotope anomaly, isotope excess etc. have been used in the historic and recent literature, but are often not carefully distinguished. The realisation that MDF comprises a range of possible relationships between the isotopes of one element led to further complications because it meant that apparent isotope anomalies could be created by a combination of different MDF processes. At the moment, at least four different definitions to quantify isotope anomalies are being used. Furthermore, coefficients used in these definitions vary, which makes the comparison of data from different sources very difficult, even for experts. A consistent set of recommendations on how to express and quantify the isotope distribution in elements with more than two stable isotopes is highly warranted. From our experience as academic teachers, we are woefully aware how impenetrable the field is for

  20. Regional variation in mercury and stable isotopes of red snapper (Lutjanus campechanus) in the northern Gulf of Mexico, USA.

    Science.gov (United States)

    Zapp Sluis, Michelle; Boswell, Kevin M; Chumchal, Matthew M; Wells, R J David; Soulen, Brianne; Cowan, James H

    2013-02-01

    The presence of total mercury (Hg) in fish tissue and the potential associated health risks has become a global concern in marine ecosystems. Few studies have examined basin-scale variation in Hg accumulation in marine ecosystems, and determining if Hg concentrations in fish tissue vary across marine ecosystems is a key monitoring question. The present study evaluated Hg concentrations in red snapper (Lutjanus campechanus) tissue across three regions of the northern Gulf of Mexico (Alabama, Louisiana, and Texas, USA) and between two habitat types (oil and gas platforms and nonplatforms) within each region. Nitrogen (δ(15)N), carbon (δ(13)C), and sulfur (δ(34)S) stable isotopes were used to investigate ecological differences that may affect Hg concentrations among regions and between habitats. Mercury concentrations in red snapper tissue were positively correlated with fish total length. Regional differences in Hg concentrations were significant, with fish collected from Alabama having the highest concentrations and fish collected from Louisiana having the lowest. No significant difference existed in Hg concentrations between habitats, suggesting that association with platforms may not be a significant factor contributing to red snapper Hg concentrations. While δ(15)N did not differ significantly among the three regions, Texas red snapper were more enriched in δ(34)S and depleted in δ(13)C compared with Alabama and Louisiana red snapper. Although the majority of red snapper collected in the present study had Hg concentrations below safe consumption guidelines, regional differences suggest that spatially explicit monitoring programs may be important for basin-wide assessments.

  1. Future stable water isotope projection with an isotope-AGCM driven by CMIP5 SSTs

    Science.gov (United States)

    Yoshimura, K.

    2016-12-01

    Stable water isotope ratios (dD and d18O) are widely used as proxy of past climate changes, and it is extremely important to understand and predict the mechanism of current isotopic spatio-temporal behavior with regard to the on-going climate change. However, as compared many studies on reproduction of isotopes for the past, there are few studies on future projection of isotopes. Therefore, in this study, a set of experiments using an isotope-incorporate AGCM (IsoGSM) with SST and sea ice field simulated from multiple CMIP5 models, namely MIROC5, CCSM4, and MRI-CGCM3, were conducted for the end of 20th century (1980-1990) and the end of 21st century (2080-2090) under RCP2.6 and RCP8.5 scenarios. Thus the responses in stable water isotope ratio in precipitation and water vapor in accordance to the global warming were investigated. As results, the changes in global surface air temperature were about +1K and +3K with RCP2.6 and RCP8.5, respectively. Similarly, the global precipitation changes were about +0.07mm/day (about +2%) and +0.18mm/day (about +5%), and the global precipitable water changes were about +2mm (+7%) and +6mm (+24%), respectively. The moisture was increased in accordance to the Clausius-Clapayron theory (7%/K), but the increase in precipitation is not that large. This indicates that the global hydrological cycle was slowed down in the globally warmed experiments. On the other hand, for the isotopic signals, the changes in globally averaged d18O in precipitation were about 0.2‰ and 0.4‰, and those in precipitable water were 0.2‰ and 0.5‰, in RCP2.6 and RCP8.5, respectively. It is well-known that there are temperature effect (positive correlation in air temperature and precipitation isotopes) and amount effect (negative correlation in precipitation amount and isotopes), but in the globally warmed world, these effects were offset, and only weaker temperature effect was appeared in the global mean isotope signals. Regional details will be shown

  2. Stable isotopes of nitrogen in plants of contaminated soils and sediments by an abandoned gold mine

    Science.gov (United States)

    Becerra, O. F.; Sanchez, A.; Marmolejo, A. J.; Magallanes, V. R.

    2013-05-01

    Mining industry is an economic activity which generates high ecological impact. In the mining district "El Triunfo", the concentration of potential toxic elements (PTE: As, Cd, Hg, Sb) have exceeded 50 times allowable limits. Nowadays, environmental pollution levels can be evaluated through the use of stable isotopes of N. For this, isotopic analysis of nitrogen and concentrations of metals and metalloids were considered in the area where plants are exposed (Prosopis spp., Parkinsonia spp. and Salicornia spp.) Polluted sediments were collected over 48 km of the Las Gallinas-El Hondo-El Carrizal arroyo. PTE concentrations, with a previous acidic digestion (HF, HCl, HNO3 and H2SO4) were determined by ICP-MS. As and Sb were determined by NAA. For N isotopes, obtained samples were grounded to fine powder in an agate mortar with an acetone rinse between samples then analyzed by an EA-IRMS. Results showed that plants growing on the tailings decreased their δ15N proportionally to the metal concentration in the area.

  3. Applications of stable isotopes to study plant-animal relationships in terrestrial ecosystems

    Institute of Scientific and Technical Information of China (English)

    WANG Jianzhu; LIN Guanghui; HUANG Jianhui; HAN Xingguo

    2004-01-01

    As natural tracers, stable isotopes have been extensively used in plant physiological, ecological and environmental research. Recently, animal physiological ecologists have also applied stable isotope techniques to study plant- animal relationships. The isotopic compositions of animal body generally reflect and integrate their diets over a time period ranging from hours to years to the lifetime of an individual. When animal living habitat changes or animals move to a new environment, the animal isotopic compositions will shift accordingly. Thus, stable isotope signatures of an animal can truly reflect its food sources, habitat, distribution and movement patterns during a given time period. Moreover, by analyzing animal-tissue isotopic compositions at different temporal scales, we can improve our understanding of animal adaptation to environmental changes. Stable isotope technique also provides an ideal tool to study animal foodweb relationship and community structure because of isotopic fractionation during the processes of nutrient assimilation by animals. Stable isotope technique can continuously measure animal trophic position in a foodweb, which can eventually reveal the predator-prey relationship and its role in determining matter balance and energy flow in the entire ecosystem. Stable isotope technique has been one of the most important and efficient tools in studying plant-animal relationship. In this paper, we first review recent advances in the application of stable isotope techniques to plant-animal relationship research then evaluate their advantages and disadvantages, and finally discuss some future directions associated with stable isotope applications to plant-animal relationship research.

  4. Using phylogenetic probes for quantification of stable isotope labeling and microbial community analysis

    Energy Technology Data Exchange (ETDEWEB)

    Brodie, Eoin L; DeSantis, Todd Z; Karaoz, Ulas; Andersen, Gary L

    2014-12-09

    Herein is described methods for a high-sensitivity means to measure the incorporation of stable isotope labeled substrates into RNA following stable isotope probing experiments (SIP). RNA is hybridized to a set of probes such as phylogenetic microarrays and isotope incorporation is quantified such as by secondary ion mass spectrometer imaging (NanoSIMS).

  5. A new method for stable lead isotope extraction from seawater

    Energy Technology Data Exchange (ETDEWEB)

    Zurbrick, Cheryl M., E-mail: CZurbric@ucsc.edu [WIGS, Department of Microbiology and Environmental Toxicology, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064 (United States); Gallon, Céline [Institute of Marine Sciences, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064 (United States); Flegal, A. Russell [WIGS, Department of Microbiology and Environmental Toxicology, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064 (United States); Institute of Marine Sciences, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064 (United States)

    2013-10-24

    Graphical abstract: -- Highlights: •We present a relatively fast (2.5–6.5 h), semi-automated system to extract Pb from seawater. •Extraction requires few chemicals and has a relatively low blank (0.7 pmol kg{sup −1}). •We compare analyses of Pb isotopes by HR ICP-MS with those by MC-ICP-MS. -- Abstract: A new technique for stable lead (Pb) isotope extraction from seawater is established using Toyopearl AF-Chelate 650 M{sup ®} resin (Tosoh Bioscience LLC). This new method is advantageous because it is semi-automated and relatively fast; in addition it introduces a relatively low blank by minimizing the volume of chemicals used in the extraction. Subsequent analyses by HR ICP-MS have a good relative external precision (2σ) of 3.5‰ for {sup 206}Pb/{sup 207}Pb, while analyses by MC-ICP-MS have a better relative external precision of 0.6‰. However, Pb sample concentrations limit MC-ICP-MS analyses to {sup 206}Pb, {sup 207}Pb, and {sup 208}Pb. The method was validated by processing the common Pb isotope reference material NIST SRM-981 and several GEOTRACES intercalibration samples, followed by analyses by HR ICP-MS, all of which showed good agreement with previously reported values.

  6. Protein Stable Isotope Fingerprinting (P-SIF): Multidimensional Protein Chromatography Coupled to Stable Isotope-Ratio Mass Spectrometry

    Science.gov (United States)

    Pearson, A.; Bovee, R. J.; Mohr, W.; Tang, T.

    2012-12-01

    As metagenomics increases our insight into microbial community diversity and metabolic potential, new approaches are required to determine the biogeochemical expression of this potential within ecosystems. Because stable isotopic analysis of the major bioactive elements (C, N) has been used historically to map flows of substrates and energy among macroscopic food webs, similar principles may apply to microbes. To address this challenge, we have developed a new analytical approach called Protein Stable Isotope Fingerprinting (P-SIF). P-SIF generates natural stable isotopic fingerprints of microbial individual or community proteomes. The main advantage of P-SIF is the potential to bridge the gap between diversity and function, thereby providing a window into the "black box" of environmental microbiology and helping to decipher the roles of uncultivated species. Our method implements a three-way, orthogonal scheme to separate mixtures of whole proteins into subfractions dominated by single or closely-related proteins. Protein extracts first are isoelectrically focused in a gel-free technique that yields 12 fractions separated over a gradient of pH 3-10. Each fraction then is separated by size-exclusion chromatography into 20 pools, ranging from >100kD to ~10kD. Finally, each of these pools is subjected to HPLC and collected in 40 time-slices based on protein hydrophobicity. Theoretical calculation reveals that the true chromatographic resolution of the total scheme is 5000, somewhat less than the 9600 resulting fractions. High-yielding fractions are subjected to δ13C analysis by spooling-wire microcombustion irMS (SWiM-irMS) optimized for samples containing 1-5 nmol carbon. Here we will present the method, results for a variety of pure cultures, and preliminary data for a sample of mixed environmental proteins. The data show the promise of this method for unraveling the metabolic complexity hidden within microbial communities.

  7. Quantitative proteomics using reductive dimethylation for stable isotope labeling.

    Science.gov (United States)

    Tolonen, Andrew C; Haas, Wilhelm

    2014-07-01

    Stable isotope labeling of peptides by reductive dimethylation (ReDi labeling) is a method to accurately quantify protein expression differences between samples using mass spectrometry. ReDi labeling is performed using either regular (light) or deuterated (heavy) forms of formaldehyde and sodium cyanoborohydride to add two methyl groups to each free amine. Here we demonstrate a robust protocol for ReDi labeling and quantitative comparison of complex protein mixtures. Protein samples for comparison are digested into peptides, labeled to carry either light or heavy methyl tags, mixed, and co-analyzed by LC-MS/MS. Relative protein abundances are quantified by comparing the ion chromatogram peak areas of heavy and light labeled versions of the constituent peptide extracted from the full MS spectra. The method described here includes sample preparation by reversed-phase solid phase extraction, on-column ReDi labeling of peptides, peptide fractionation by basic pH reversed-phase (BPRP) chromatography, and StageTip peptide purification. We discuss advantages and limitations of ReDi labeling with respect to other methods for stable isotope incorporation. We highlight novel applications using ReDi labeling as a fast, inexpensive, and accurate method to compare protein abundances in nearly any type of sample.

  8. Stable isotope studies. Final report, March 1, 1972--February 29, 1992

    Energy Technology Data Exchange (ETDEWEB)

    Ishida, T.

    1992-10-01

    The research has been in four general areas: (1) correlation of isotope effects with molecular forces and molecular structures, (2) correlation of zero-point energy and its isotope effects with molecular structure and molecular forces, (3) vapor pressure isotope effects, and (4) fractionation of stable isotopes. 73 refs, 38 figs, 29 tabs.

  9. Developing stable isotopic records from organic material preserved in speleothems

    Science.gov (United States)

    Blyth, A.; Baker, A.

    2011-12-01

    Speleothems form excellent archives of environmental change as they are easy to date and preserve multiple environmental records. However, although the stable isotopic composition of speleothem calcite is well characterised, the isotopic composition of organic matter in speleothems has not been investigated in-depth. The approach has considerable potential value in providing isotopic records directly linked to the overlying ecosystem. For stable carbon isotopes, this will provide records independent of the calcite signal, and enable identification of the local primary controls (vegetation type, climate, soil conditions). However, the detailed measurement of isotopic records in speleothem organic matter is hindered by methodological constraints, including extraction of sufficient material, and recovery without adding carbon contamination. Here we present results from a number of methodological techniques and discuss which show most potential for future palaeoenvironmental work. Isotopic records in organic matter can be divided into two main types - analyses of the bulk organic fraction, and compound-specific analyses of molecules of interest (e.g. plant waxes, bacterial lipids etc.). From the point of view of sample size, bulk analysis is the most amenable. Our work shows that TOC in stalagmites can be as low as sample size of between 100 and 500 mg of calcite powder, which provides a workable temporal resolution at a decadal-centennial scale on many samples. The main obstacle is the recovery of bulk organics without carbon contamination, but whilst avoiding excess acid salts which might damage the instrumentation. One method we propose is the use of an iTOC-CDRS analyser, which measures total organic carbon, and also 12C and 13C isotopes. The approach has the advantage of requiring minimal sample preparation, with drilled powders simply being dissolved in acid. The major limitation is the precision on the CDRS, which at +/- 0.15% does not match that of IRMS

  10. Subterranean sympatry: an investigation into diet using stable isotope analysis.

    Directory of Open Access Journals (Sweden)

    Gillian N Robb

    Full Text Available In the Western Cape three species of mole-rat occur in sympatry, however, little is known about differences in their dietary preferences. Dietary composition of the three species; the common mole-rat (Cryptomys hottentotus hottentotus, the Cape mole-rat (Georychus capensis and the Cape dune mole-rat (Bathyergus suillus were examined using stable isotope analysis. Blood, fur and claw samples were collected from 70 mole-rats, in addition to several potential food items, to assess food selection of the three species under natural conditions. Overall there was a significant difference in the isotopic composition (δ(13C and δ(15N between all three species and significant differences in their diet composition. There were also significant differences between tissues in all three species suggesting temporal variation in diet. The small size and colonial lifestyle of C. h. hottentotus allows it to feed almost 100% on bulbs, while the solitary and larger species G. capensis and B. suillus fed to a greater extent on other resources such as grasses and clover. B. suillus, the largest of the species, had the most generalized diet. However, overall all species relied most heavily upon geophytes and consumed the same species suggesting competition for resources could exist. We also showed a high level of individual variation in diet choices. This was most pronounced in B. suillus and G. capensis and less so in C. h. hottentotus. We demonstrate that stable isotope analysis can successfully be applied to examine dietary patterns in subterranean mammals and provide insights into foraging patterns and dietary variation at both the inter and intra population level.

  11. Electromagnetic separation of stable isotopes at China Institute of Atomic Energy

    Science.gov (United States)

    Meiqin, Xiao; Hongyou, Lu; Shijun, Su; Zhizhou, Lin

    1993-09-01

    Electromagnetic separation of stable isotopes at CIAE is described. The separators, the ion sources used, the isotopes separated and their applications are introduced. The improvement of a 180° production separator is also described.

  12. STABLE ISOTOPES IN ECOLOGICAL STUDIES: NEW DEVELOPMENTS IN MIXING MODELS (URUGUAY)

    Science.gov (United States)

    Stable isotopes are increasingly being used as tracers in ecological studies. One application uses isotopic ratios to quantify the proportional contributions of multiple sources to a mixture. Examples include pollution sources for air or water bodies, food sources for animals, ...

  13. /sup 203/Hg and other gamma-emitting radio-isotopes as labels for Dirofilaria immitis microfilariae

    Energy Technology Data Exchange (ETDEWEB)

    Lengemann, F.W.; Grieve, R.B.; Chmielewicz, M.; Georgi, J.R.

    1986-04-01

    The in vitro uptake of gamma-emitting radionuclides by microfilariae of Dirofilaria immitis was investigated. Radionuclides tested were /sup 133/Ba, /sup 207/Bi, /sup 82/Br, /sup 109/Cd, /sup 51/Cr, /sup 60/Co, /sup 59/Fe, /sup 203/Hg, /sup 125/I, /sup 54/Mn, /sup 32/P, /sup 125/Sb, /sup 46/Sc, /sup 75/Se and /sup 65/Zn. Only /sup 207/Bi, /sup 59/Fe, /sup 203/Hg, /sup 54/Mn and /sup 46/Sc showed more than 2% of the available radioactivity to bind to the microfilariae. When tested for retention in vitro only /sup 203/Hg showed retention levels approaching 90%. Moreover, when dimethyl-sulphoxide was incorporated into the medium at levels of 1% (v/v) the uptake of /sup 203/Hg could be increased by 3-5 times; no other radio-isotope tested responded in this manner. The uptake of /sup 203/Hg was directly related to temperature and time of incubation. Mercury, as mercuric chloride, was toxic to the microfilariae and represents an impediment to the incorporation of high levels of /sup 203/Hg in microfilariae.

  14. Multivariate Stable Isotope Analysis to Determine Linkages between Benzocaine Seizures

    Science.gov (United States)

    Kemp, H. F.; Meier-Augenstein, W.; Collins, M.; Salouros, H.; Cunningham, A.; Harrison, M.

    2012-04-01

    In July 2010, a woman was jailed for nine years in the UK after the prosecution successfully argued that attempting to import a cutting agent was proof of involvement in a conspiracy to supply Cocaine. That landmark ruling provided law enforcement agencies with much greater scope to tackle those involved in this aspect of the drug trade, specifically targeting those importing the likes of benzocaine or lidocaine. Huge quantities of these compounds are imported into the UK and between May and August 2010, four shipments of Benzocaine amounting to more then 4 tons had been seized as part of Operation Kitley, a joint initiative between the UK Border Agency and the Serious Organised Crime Agency (SOCA). By diluting cocaine, traffickers can make it go a lot further for very little cost, leading to huge profits. In recent years, dealers have moved away from inert substances, like sugar and baby milk powder, in favour of active pharmaceutical ingredients (APIs), including anaesthetics like Benzocaine and Lidocaine. Both these mimic the numbing effect of cocaine, and resemble it closely in colour, texture and some chemical behaviours, making it easier to conceal the fact that the drug has been diluted. API cutting agents have helped traffickers to maintain steady supplies in the face of successful interdiction and even expand the market in the UK, particularly to young people aged from their mid teens to early twenties. From importation to street-level, the purity of the drug can be reduced up to a factor of 80 and street level cocaine can have a cocaine content as low as 1%. In view of the increasing use of Benzocaine as cutting agent for Cocaine, a study was carried out to investigate if 2H, 13C, 15N and 18O stable isotope signatures could be used in conjunction with multivariate chemometric data analysis to determine potential linkage between benzocaine exhibits seized from different locations or individuals to assist with investigation and prosecution of drug

  15. Characterization of phenols biodegradation by compound specific stable isotope analysis

    Science.gov (United States)

    Wei, Xi; Gilevska, Tetyana; Wenzig, Felix; Hans, Richnow; Vogt, Carsten

    2015-04-01

    -cresol degradation and 2.2±0.3‰ for m-cresol degradation, respectively. The carbon isotope fractionation patterns of phenol degradation differed more profoundly. Oxygen-dependent monooxygenation of phenol by A.calcoaceticus as the initial reaction yielded ƐC values of -1.5±0.02‰. In contrast, the anaerobic degradation initiated by ATP-dependent carboxylation performed by Thauera aromatia DSM 6984, produced no detectable fractionation (ƐC 0±0.1‰). D. cetonica showed a slight inverse carbon isotope fractionation (ƐC 0.4±0.1‰). In conclusion, a validated method for compound specific stable isotope analysis was developed for phenolic compounds, and the first data set of carbon enrichment factors upon the biodegradation of phenol and cresols with different activation mechanisms has been obtained in the present study. Carbon isotope fractionation analysis is a potentially powerful tool to monitor phenolic compounds degradation in the environment.

  16. Expanding the isotopic toolbox: Applications of hydrogen and oxygen stable isotope ratios to food web studies

    Directory of Open Access Journals (Sweden)

    Hannah B Vander Zanden

    2016-03-01

    Full Text Available The measurement of stable carbon (δ13C and nitrogen (δ15N isotopes in tissues of organisms has formed the foundation of isotopic food web reconstructions, as these values directly reflect assimilated diet. In contrast, stable hydrogen (δ2H and oxygen (δ18O isotope measurements have typically been reserved for studies of migratory origin and paleoclimate reconstruction based on systematic relationships between organismal tissue and local environmental water. Recently, innovative applications using δ2H and, to a lesser extent, δ18O values have demonstrated potential for these elements to provide novel insights in modern food web studies. We explore the advantages and challenges associated with three applications of δ2H and δ18O values in food web studies. First, large δ2H differences between aquatic and terrestrial ecosystem end members can permit the quantification of energy inputs and nutrient fluxes between these two sources, with potential applications for determining allochthonous vs. autochthonous nutrient sources in freshwater systems and relative aquatic habitat utilization by terrestrial organisms. Next, some studies have identified a relationship between δ2H values and trophic position, which suggests that this marker may serve as a trophic indicator, in addition to the more commonly used δ15N values. Finally, coupled measurements of δ2H and δ18O values are increasing as a result of reduced analytical challenges to measure both simultaneously and may provide additional ecological information over single element measurements. In some organisms, the isotopic ratios of these two elements are tightly coupled, whereas the isotopic disequilibrium in other organisms may offer insight into the diet and physiology of individuals. Although a coherent framework for interpreting δ2H and δ18O data in the context of food web studies is emerging, many fundamental uncertainties remain. We highlight directions for targeted research that

  17. Stable nitrogen isotopes in coastal macroalgae: geographic and anthropogenic variability.

    Science.gov (United States)

    Viana, Inés G; Bode, Antonio

    2013-01-15

    Growing human population adds to the natural nitrogen loads to coastal waters. Both anthropogenic and natural nitrogen is readily incorporated in new biomass, and these different nitrogen sources may be traced by the measurement of the ratio of stable nitrogen isotopes (δ(15)N). In this study δ(15)N was determined in two species of macroalgae (Ascophyllum nodosum and Fucus vesiculosus), and in nitrate and ammonium to determine the relative importance of anthropogenic versus natural sources of nitrogen along the coast of NW Spain. Both algal species and nitrogen sources showed similar isotopic enrichment for a given site, but algal δ(15)N was not related to either inorganic nitrogen concentrations or δ(15)N in the water samples. The latter suggests that inorganic nitrogen inputs are variable and do not always leave an isotopic trace in macroalgae. However, a significant linear decrease in macroalgal δ(15)N along the coast is consistent with the differential effect of upwelling. Besides this geographic variability, the influence of anthropogenic nitrogen sources is evidenced by higher δ(15)N in macroalgae from rias and estuaries compared to those from open coastal areas and in areas with more than 15×10(3) inhabitants in the watershed. These results indicate that, in contrast with other studies, macroalgal δ(15)N is not simply related to either inorganic nitrogen concentrations or human population size but depends on other factors as the upwelling or the efficiency of local waste treatment systems. Copyright © 2012 Elsevier B.V. All rights reserved.

  18. Stable nitrogen isotopes in coastal macroalgae: Geographic and anthropogenic variability

    Energy Technology Data Exchange (ETDEWEB)

    Viana, Inés G., E-mail: ines.gonzalez@co.ieo.es; Bode, Antonio

    2013-01-15

    Growing human population adds to the natural nitrogen loads to coastal waters. Both anthropogenic and natural nitrogen is readily incorporated in new biomass, and these different nitrogen sources may be traced by the measurement of the ratio of stable nitrogen isotopes (δ{sup 15}N). In this study δ{sup 15}N was determined in two species of macroalgae (Ascophyllum nodosum and Fucus vesiculosus), and in nitrate and ammonium to determine the relative importance of anthropogenic versus natural sources of nitrogen along the coast of NW Spain. Both algal species and nitrogen sources showed similar isotopic enrichment for a given site, but algal δ{sup 15}N was not related to either inorganic nitrogen concentrations or δ{sup 15}N in the water samples. The latter suggests that inorganic nitrogen inputs are variable and do not always leave an isotopic trace in macroalgae. However, a significant linear decrease in macroalgal δ{sup 15}N along the coast is consistent with the differential effect of upwelling. Besides this geographic variability, the influence of anthropogenic nitrogen sources is evidenced by higher δ{sup 15}N in macroalgae from rias and estuaries compared to those from open coastal areas and in areas with more than 15 × 10{sup 3} inhabitants in the watershed. These results indicate that, in contrast with other studies, macroalgal δ{sup 15}N is not simply related to either inorganic nitrogen concentrations or human population size but depends on other factors as the upwelling or the efficiency of local waste treatment systems. - Highlights: ► Anthropogenic versus upwelling nitrogen effect on macroalgal δ{sup 15}N was studied. ► The influence of populations and upwelling has not been made before on macroalgal δ{sup 15}N. ► Natural variability has not been taken into account in most biomonitoring studies. ► Upwelling explains most of the variability in δ{sup 15}N in macroalgae.

  19. Application of stable isotope analysis for detecting filial cannibalism.

    Science.gov (United States)

    Sogabe, Atsushi; Hamaoka, Hideki; Fukuta, Atsushi; Shibata, Jun-Ya; Shoji, Jun; Omori, Koji

    2017-07-01

    A novel type of filial cannibalism has been reported in pipefishes, in which the eggs are absorbed through the male's brood-pouch epithelium. The present study explored the applicability of stable isotope analysis for the detection of paternal brood cannibalism in the seaweed pipefish Syngnathus schlegeli. As expected, the δ(15)N values for liver, which conveys short-term dietary information about the recent reproductive season, were higher in males than in females. In contrast, the δ(15)N values for muscle, which reflects longer-term feeding habits that span both the reproductive and non-reproductive seasons, did not significantly differ between the sexes. This finding indicates that males occupy a higher trophic position than females only during the reproductive season, and it is probable that this difference is a result of paternal uptake of nutrients from embryos in the brood pouch. Copyright © 2017 Elsevier B.V. All rights reserved.

  20. Stable isotopes and turnover of nitrate in the German Bight

    Science.gov (United States)

    Dähnke, K.; Johannsen, A.; Emeis, K.

    2009-04-01

    The German Bight is a hot-spot of river-induced eutrophication of the North Sea due to nitrate loads discharged into this semi-isolated embayment by several large rivers. We analysed stable isotope signatures of water column nitrate in the area on a grid of stations in winter and early spring 2007. Overall spatial patterns of δ15N-NO3- and δ18O-NO3- image the predominant influence of the rivers Rhine and Elbe on the German Bight. On a smaller scale, however, and in offshore stations, nitrate assimilation of an incipient phytoplankton bloom is indicated by parallel enrichment of δ15N-NO3- and δ18O-NO3-. Intriguingly, the enrichment ratio in ɛ18: ɛ15 is 1.5:1, differing from the ratio of 1:1 associated with uptake by marine phytoplankton. These data suggest that the shift in nitrate isotopes is not solely due to beginning phytoplankton assimilation, but that, despite low temperatures, nitrate in the outer regions of the German Bight derives to a considerable extent from nitrification. These novel data mark remineralization in sediments as an important source of DIN and underscore the role of sediments in recharging water column nutrient inventories.

  1. Stable isotope composition of mercury forms in flue gases from a typical coal-fired power plant, Inner Mongolia, northern China.

    Science.gov (United States)

    Tang, Shunlin; Feng, Chaohui; Feng, Xinbin; Zhu, Jianming; Sun, Ruoyu; Fan, Huipeng; Wang, Lina; Li, Ruiyang; Mao, Tonghua; Zhou, Ting

    2017-04-15

    Mercury forms emitted from coal combustion via air pollution control devices are speculated to carry different Hg isotope signatures. Yet, their Hg isotope composition is still not reported. Here, we present the first onsite Hg isotope data for gaseous elemental Hg (GEM) and gaseous oxidized Hg (GOM) of flue gases from a typical lignite-fired power plant (CFPP). Significant mass dependent fractionation (MDF) and insignificant mass independent fractionation (MIF) are observed between feed coal and coal combustion products. As compared to feed coal (δ(202)Hg=-2.04±0.25‰), bottom ash, GEM and GOM in flue gases before and after wet flue gas desulfurization system significantly enrich heavy Hg isotopes by 0.7-2.6‰ in δ(202)Hg, while fly ash, desulfurization gypsum and waste water show slight but insignificant enrichment of light Hg isotopes. GEM is significantly enriched heavy Hg isotopes compared to GOM and Hg in fly ash. Our observations verify the previous speculation on Hg isotope fractionation mechanism in CFPPs, and suggest a kinetically-controlled mass dependent Hg isotope fractionation during transformation of Hg forms in flue gases. Finally, our data are compared to Hg isotope compositions of atmospheric Hg pools, suggesting that coal combustion Hg emission is likely an important atmospheric Hg contributor.

  2. Stable isotope composition of atmospheric carbon monoxide. A modelling study

    Energy Technology Data Exchange (ETDEWEB)

    Gromov, Sergey S.

    2014-11-01

    This study aims at an improved understanding of the stable carbon and oxygen isotope composition of the carbon monoxide (CO) in the global atmosphere by means of numerical simulations. At first, a new kinetic chemistry tagging technique for the most complete parameterisation of isotope effects has been introduced into the Modular Earth Submodel System (MESSy) framework. Incorporated into the ECHAM/MESSy Atmospheric Chemistry (EMAC) general circulation model, an explicit treatment of the isotope effects on the global scale is now possible. The expanded model system has been applied to simulate the chemical system containing up to five isotopologues of all carbon- and oxygen-bearing species, which ultimately determine the δ{sup 13}C, δ{sup 18}O and Δ{sup 17}O isotopic signatures of atmospheric CO. As model input, a new stable isotope-inclusive emission inventory for the relevant trace gases has been compiled. The uncertainties of the emission estimates and of the resulting simulated mixing and isotope ratios have been analysed. The simulated CO mixing and stable isotope ratios have been compared to in-situ measurements from ground-based observatories and from the civil-aircraft-mounted CARIBIC-1 measurement platform. The systematically underestimated {sup 13}CO/{sup 12}CO ratios of earlier, simplified modelling studies can now be partly explained. The EMAC simulations do not support the inferences of those studies, which suggest for CO a reduced input of the highly depleted in {sup 13}C methane oxidation source. In particular, a high average yield of 0.94 CO per reacted methane (CH{sub 4}) molecule is simulated in the troposphere, to a large extent due to the competition between the deposition and convective transport processes affecting the CH{sub 4} to CO reaction chain intermediates. None of the other factors, assumed or disregarded in previous studies, however hypothesised to have the potential in enriching tropospheric CO in {sup 13}C, were found significant

  3. Stable Isotopic Variations in Precipitation in Southwest China

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    This study analyzes the relationships of stable isotopes in precipitation with temperature, air pressure and humidity at different altitudes, and the potential influencing mechanisms of control factors on the stable isotopes in precipitation in Southwest China. There appear marked negative correlations of the δ18O in precipitation with precipitation amount, vapor pressure and atmospheric precipitable water (PW)at the Mengzi, Simao and Tengchong stations on the synoptic timescale; the marked negative correlations between the δ18O in precipitation and the diurnal mean temperature at 400 hPa, 500 hPa, 700 hPa and 850hPa are different from the temperature effect in middle-high-latitude inland areas. In addition, the notable positive correlation between the δ18O in precipitation and the dew-point deficit △Td at different altitudes is found at the three stations. Precipitation is not the only factor generating an amount effect. Probably,the amount effect is related to the variations of atmospheric circulation and vapor origins. On the annual timescale, the annual precipitation amount weighted-mean δ18O displays negative correlations not only with annual precipitation but also with annual mean temperature at 500 hPa. It can be deduced that, in the years with an abnormally strong summer monsoon, more warm and wet air from low-latitude oceans is transported northward along the vapor channel located in Southwest China and generates abnormally strong rainfall on the way. Meanwhile, the abnormally strong condensation process will release more condensed latent heat in the atmosphere, and this will lead to a rise of atmospheric temperature during rainfall but a decline of δ18O in the precipitation. On the other hand, in the years with an abnormally weak summer monsoon, the precipitation and the atmospheric temperature during rainfalls decrease abnormally but the δ18O in precipitation increases.

  4. Quantification of organic pollutant degradation in contaminated aquifers using compound specific stable isotope analysis – Review of recent developments

    NARCIS (Netherlands)

    Thullner, M.; Centler, F.; Richnow, H.-H.; Fischer, A.

    2012-01-01

    Compound specific stable isotope analysis (CSIA) has been established as a viable tool for proving, characterizing and assessing degradation of organic pollutants within contaminated aquifers. The fractionation of stable isotopes during contaminant degradation leads to observable shifts in stable is

  5. An evaluation of teeth of ringed seals (Phoca hispida) from Greenland as a matrix to monitor spatial and temporal trends of mercury and stable isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Aubail, Aurore, E-mail: aaubail@yahoo.fr [National Environmental Research Institute, Aarhus University, Frederiksborgvej 399, P.O. Box 358, DK-4000 Roskilde (Denmark); Littoral, Environnement et Societes (LIENSs), UMR 6250, CNRS-Universite de La Rochelle, 2 rue Olympe de Gouges, F-17000 La Rochelle (France); Dietz, Rune; Riget, Frank [National Environmental Research Institute, Aarhus University, Frederiksborgvej 399, P.O. Box 358, DK-4000 Roskilde (Denmark); Simon-Bouhet, Benoit; Caurant, Florence [Littoral, Environnement et Societes (LIENSs), UMR 6250, CNRS-Universite de La Rochelle, 2 rue Olympe de Gouges, F-17000 La Rochelle (France)

    2010-10-01

    Total mercury (Hg) concentrations were measured in teeth of ringed seals from Qeqertarsuaq, central West Greenland (1982 to 2006) and Ittoqqortoormiit, central East Greenland (1986 to 2006). Stable isotopic ratios of carbon ({sup 13}C/{sup 12}C) and nitrogen ({sup 15}N/{sup 14}N) were determined as well to provide insights into diet variations between regions or through time. Mercury concentrations decreased the first years of life of the animals suggesting that Hg had been transferred from the mother to the foetus and newborn. The Hg concentrations in teeth were significantly lesser in ringed seals from central West Greenland compared to those from central East Greenland. Carbon and nitrogen stable isotopic values measured in the animals differed also significantly between the two regions. Increasing temporal trends of dental Hg concentrations between 1994 and 2006 were observed in ringed seals from both central West Greenland and central East Greenland. These increases were attributed to global changes in environmental Hg levels since no temporal trends in {delta}{sup 15}N values were found to support the hypothesis of a diet shift over time. Furthermore, a decreasing temporal trend in {delta}{sup 13}C values was observed in the teeth of seals from central East Greenland, and explained by a likely change over time towards more pelagic feeding habits; alternatively, the so-known Seuss effect was thought to be responsible for this decrease. Finally, it was concluded that the tooth of ringed seal was a good monitoring tissue to assess Hg trends.

  6. An evaluation of teeth of ringed seals (Phoca hispida) from Greenland as a matrix to monitor spatial and temporal trends of mercury and stable isotopes.

    Science.gov (United States)

    Aubail, Aurore; Dietz, Rune; Rigét, Frank; Simon-Bouhet, Benoît; Caurant, Florence

    2010-10-01

    Total mercury (Hg) concentrations were measured in teeth of ringed seals from Qeqertarsuaq, central West Greenland (1982 to 2006) and Ittoqqortoormiit, central East Greenland (1986 to 2006). Stable isotopic ratios of carbon ((13)C/(12)C) and nitrogen ((15)N/(14)N) were determined as well to provide insights into diet variations between regions or through time. Mercury concentrations decreased the first years of life of the animals suggesting that Hg had been transferred from the mother to the foetus and newborn. The Hg concentrations in teeth were significantly lesser in ringed seals from central West Greenland compared to those from central East Greenland. Carbon and nitrogen stable isotopic values measured in the animals differed also significantly between the two regions. Increasing temporal trends of dental Hg concentrations between 1994 and 2006 were observed in ringed seals from both central West Greenland and central East Greenland. These increases were attributed to global changes in environmental Hg levels since no temporal trends in delta(15)N values were found to support the hypothesis of a diet shift over time. Furthermore, a decreasing temporal trend in delta(13)C values was observed in the teeth of seals from central East Greenland, and explained by a likely change over time towards more pelagic feeding habits; alternatively, the so-known Seuss effect was thought to be responsible for this decrease. Finally, it was concluded that the tooth of ringed seal was a good monitoring tissue to assess Hg trends.

  7. Quantifying nitrogen process rates in a constructed wetland using natural abundance stable isotope signatures and stable isotope amendment experiments.

    Science.gov (United States)

    Erler, Dirk V; Eyre, Bradley D

    2010-01-01

    This study describes the spatial variability in nitrogen (N) transformation within a constructed wetland (CW) treating domestic effluent. Nitrogen cycling within the CW was driven by settlement and mineralization of particulate organic nitrogen and uptake of NO3-. The concentration of NO3- was found to decrease, as the delta15N-NO3- signature increased, as water flowed through the CW, allowing denitrification rates to be estimated on the basis of the degree of fractionation of delta15N-NO3-. Estimates of denitrification hinged on the determination of a net isotope effect (eta), which was influenced byprocesses that enrich or deplete 15NO3- (e.g., nitrification), as well as the rate constants associated with the different processes involved in denitrification (i.e., diffusion and enzyme activity). The influence of nitrification on eta was quantified; however, it remained unclear how eta varied due to variability in denitrification rate constants. A series of stable isotope amendment experiments was used to further constrain the value of eta and calculate rates of denitrification, and nitrification, within the wetland. The maximum calculated rate of denitrification was 956 +/- 187 micromol N m(-2) h(-1), and the maximum rate of nitrification was 182 +/- 28.9 micromol N m(-2) h(-1). Uptake of NO3- was quantitatively more important than denitrification throughoutthe wetland. Rates of N cycling varied spatially within thewetland, with denitrification dominating in the downstream deoxygenated region of the wetland. Studies that use fractionation of N to derive rate estimates must exercise caution when interpreting the net isotope effect. We suggest a sampling procedure for future natural abundance studies that may help improve the accuracy of N cycling rate estimates.

  8. Environmental controls on stable isotope ratios in New Zealand Podocarpaceae: Implications for palaeoclimate reconstruction

    Science.gov (United States)

    Brett, Marianne J.; Baldini, James U. L.; Gröcke, Darren R.

    2014-09-01

    Stable isotope ratios of various proxies are widely used for palaeoclimate reconstruction, and it is often assumed that isotope ratios reflect vegetation abundance or type. However, very little research exists on the isotopic equilibration of extant biomes under variable environmental conditions. In this study, carbon and oxygen isotope ratios from leaves of various Podocarpaceae genera, endemic to New Zealand, are linked to environmental parameters from the Land Environments New Zealand model. The dominant influence on stable isotope ratios within the majority of Podocarpaceae studied here is vapour pressure deficit (VPD). A simple latitudinal trend does not exist, and neither temperature nor rainfall (decoupled from VPD) controls the stable isotope ratios. The results suggest that modern spatial heterogeneity in VPD affects the stable isotope values of vegetation, and that historic VPD variability would change the stable isotope ratios of Podocarpaceae without necessitating a change in vegetation type, density, or productivity. This represents an alternative model for temporal isotope change within geochemical proxies and reinforces the need for increased stable isotopic research in modern plant ecosystems to better understand modern, and eventually palaeoclimatic processes affecting the terrestrial biosphere.

  9. A Teaching Exercise to Introduce Stable Isotope Fractionation of Metals into Geochemistry Courses

    Science.gov (United States)

    Weiss, Dominik J.; Harris, Caroline; Maher, Kate; Bullen, Thomas

    2013-01-01

    Variations in the isotopic composition of elements have been widely used to study earth's climate, biosphere, and interior, and more recently to track the fate of contaminants. Within the broad range of elements that exhibit measureable isotopic variations, metal stable isotopes are increasingly applied across the biological, geological,…

  10. Stable isotope customer list and summary of shipments, FY 1985. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Tracy, J.G. (comp.)

    1986-01-01

    This compilation is published as an aid to those concerned with the separation and sale of stable isotopes. The information is divided into four sections: (1) alphabetical list of domestic and foreign customers, showing the stable isotopes purchased during the fiscal year; (2) alphabetical list of isotopes, cross-referenced to customer numbers and divided into domestic and foreign categories; (3) alphabetical list of states and countries, cross-referenced to customer numbers and indicating geographical concentrations of isotope users; and (4) tabulation of the shipments, quantities, and dollars for domestic, foreign, and project categories for each isotope.

  11. Stable-isotope customer list and summary of shipments, FY 1982

    Energy Technology Data Exchange (ETDEWEB)

    Davis, W.C. (comp.)

    1983-04-01

    This compilation is published as an aid to those concerned with the separation and sale of stable isotopes. The inforamtion is divided into four sections: (1) alphabetical list of domestic and foreign customers, showing the stable isotopes purchased during the fiscal year; (2) alphabetical list of isotopes, cross-referenced to customer numbers and divided into domestic and foreign categories; (3) alphabetical list of states and countries, cross-referenced to customer numbers and indicating geographical concentrations of isotope users; and (4) tabulation of the shipments, quantities, and dollars for domestic, foreign, and project categories for each isotope.

  12. Experimental study on stable isotopic fractionation of evaporating water under varying temperature

    Institute of Scientific and Technical Information of China (English)

    Hai-ying HU; Wei-min BAO; Tao WANG; Si-min QU

    2009-01-01

    The variation of stable isotope ratios in natural waters provides valuable information that can be used to trace water movement. Evaporation plays a crucial role in determining the variation of stable isotopes. In this paper, several evaporation experiments were conducted in order to study the stable isotopic fractionation mechanism of water and analyze the influence of different temperatures on evaporation fractionation. Three group experiments of water evaporation under different temperatures and initial isotopic values were carried out. The results show that fractionation factors of hydrogen and oxygen may increase with temperature, and the average enrichment degree of hydrogen isotope D is 3.432 times that of oxygen isotope 18O. The results also show that the isotopic composition of the initial water has little influence on water evaporation fractionation, which is mainly affected by the state variables in the evaporation process, such as temperature. This research provides experimental data for further understanding the evaporation fractionation mechanism.

  13. Forensic applications of light-element stable isotope ratios of Ricinus communis seeds and ricin preparations.

    Science.gov (United States)

    Kreuzer, Helen W; West, Jason B; Ehleringer, James R

    2013-01-01

    Seeds of the castor plant Ricinus communis are of forensic interest because they are the source of the poison ricin. We tested whether stable isotope ratios of castor seeds and ricin preparations can be used as a forensic signature. We collected over 300 castor seed samples worldwide and measured the C, N, O, and H isotope ratios of the whole seeds and oil. We prepared ricin by three different procedures, acetone extraction, salt precipitation, and affinity chromatography, and compared their isotope ratios to those of the source seeds. The N isotope ratios of the ricin samples and source seeds were virtually identical. Therefore, N isotope ratios can be used to correlate ricin prepared by any of these methods to source seeds. Further, stable isotope ratios distinguished >99% of crude and purified ricin protein samples in pairwise comparison tests. Stable isotope ratios therefore constitute a valuable forensic signature for ricin preparations. © 2012 American Academy of Forensic Sciences.

  14. Seasonal variation in stable carbon and nitrogen isotope values of bats reflect environmental baselines.

    Directory of Open Access Journals (Sweden)

    Ana G Popa-Lisseanu

    Full Text Available The stable carbon and nitrogen isotope composition of animal tissues is commonly used to trace wildlife diets and analyze food chains. Changes in an animal's isotopic values over time are generally assumed to indicate diet shifts or, less frequently, physiological changes. Although plant isotopic values are known to correlate with climatic seasonality, only a few studies restricted to aquatic environments have investigated whether temporal isotopic variation in consumers may also reflect environmental baselines through trophic propagation. We modeled the monthly variation in carbon and nitrogen isotope values in whole blood of four insectivorous bat species occupying different foraging niches in southern Spain. We found a common pattern of isotopic variation independent of feeding habits, with an overall change as large as or larger than one trophic step. Physiological changes related to reproduction or to fat deposition prior to hibernation had no effect on isotopic variation, but juvenile bats had higher δ13C and δ15N values than adults. Aridity was the factor that best explained isotopic variation: bat blood became enriched in both 13C and 15N after hotter and/or drier periods. Our study is the first to show that consumers in terrestrial ecosystems reflect seasonal environmental dynamics in their isotope values. We highlight the danger of misinterpreting stable isotope data when not accounting for seasonal isotopic baselines in food web studies. Understanding how environmental seasonality is integrated in animals' isotope values will be crucial for developing reliable methods to use stable isotopes as dietary tracers.

  15. Uniform stable-isotope labeling in mammalian cells: formulation of a cost-effective culture medium

    NARCIS (Netherlands)

    Egorova-Zachernyuk, T.A.; Bosman, G.J.C.G.M.; Grip, W.J. de

    2011-01-01

    Uniform stable-isotope labeling of mammalian cells is achieved via a novel formulation of a serum-free cell culture medium that is based on stable-isotope-labeled autolysates and lipid extracts of various microbiological origin. Yeast autolysates allow complete replacement of individual amino acids

  16. Bioavailability of xenobiotics in unsaturated soils – implications for nucleic acid based stable isotope probing

    Science.gov (United States)

    The use of stable isotopes to label phylogenetically informative biomolecules (phospholipid fatty acids, DNA, or RNA), typically referred to as stable isotope probing (SIP) has the potential of providing definitive evidence that a detected population is active in a specific process, if that process ...

  17. Uniform stable-isotope labeling in mammalian cells: formulation of a cost-effective culture medium

    NARCIS (Netherlands)

    Egorova-Zachernyuk, T.A.; Bosman, G.J.C.G.M.; Grip, W.J. de

    2011-01-01

    Uniform stable-isotope labeling of mammalian cells is achieved via a novel formulation of a serum-free cell culture medium that is based on stable-isotope-labeled autolysates and lipid extracts of various microbiological origin. Yeast autolysates allow complete replacement of individual amino acids

  18. IsoBank – Stable isotope ecology in the age of ‘Big Data’

    Science.gov (United States)

    Stable isotopes ratios provide valuable information to fish biologists working in a diverse range of fields: e.g. ecologists, population biologists and fishery managers. Ecologists take advantage of stable isotope ratios to provide information on the diet and migration history of consumers or when a...

  19. Do stable carbon isotopes of brown coal woods record changes in Lower Miocene palaeoecology?

    NARCIS (Netherlands)

    Poole, I.J.; Dolezych, M.; Kool, J.; Burgh, J. van der; Bergen, P.F. van

    2006-01-01

    Stable carbon isotope ratios of fossil wood from the Miocene brown coal deposits in former East Germany are compared with palaeobotanical and sedimentological data to test the use of stable isotopes in determining palaeoenvironment. Significant differences in the chemical composition of samples from

  20. Specificity of synthesis and analysis of molecules labelled with stable isotopes; Specificite des syntheses et analyses des molecules marquees avec les isotopes stables

    Energy Technology Data Exchange (ETDEWEB)

    Noel, J.P. [CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France). Dept. de Biologie Cellulaire et Moleculaire

    1994-12-31

    The various specificities of synthesis with stable isotopes are: unusual raw materials and use of precursors, possibility of producing several isotopomers corresponding to the same chemical compound, involvement of a reaction mechanism in labelling. As a matter of fact, isotopic synthesis are generally more complex than synthesis of non-labelled products. When preparing labelled molecules, it is of importance to avoid any isotopic dilution. Chemical purity for labelled molecules is controlled by various chromatographic techniques and isotopic analysis are carried out by nuclear magnetic resonance and mass spectrometry. 9 figs., 2 tabs.

  1. Stable water isotope patterns in a climate change hotspot: the isotope hydrology framework of Corsica (western Mediterranean).

    Science.gov (United States)

    van Geldern, Robert; Kuhlemann, Joachim; Schiebel, Ralf; Taubald, Heinrich; Barth, Johannes A C

    2014-06-01

    The Mediterranean is regarded as a region of intense climate change. To better understand future climate change, this area has been the target of several palaeoclimate studies which also studied stable isotope proxies that are directly linked to the stable isotope composition of water, such as tree rings, tooth enamel or speleothems. For such work, it is also essential to establish an isotope hydrology framework of the region of interest. Surface waters from streams and lakes as well as groundwater from springs on the island of Corsica were sampled between 2003 and 2009 for their oxygen and hydrogen isotope compositions. Isotope values from lake waters were enriched in heavier isotopes and define a local evaporation line (LEL). On the other hand, stream and spring waters reflect the isotope composition of local precipitation in the catchment. The intersection of the LEL and the linear fit of the spring and stream waters reflect the mean isotope composition of the annual precipitation (δP) with values of-8.6(± 0.2) ‰ for δ(18)O and-58(± 2) ‰ for δ(2)H. This value is also a good indicator of the average isotope composition of the local groundwater in the island. Surface water samples reflect the altitude isotope effect with a value of-0.17(± 0.02) ‰ per 100 m elevation for oxygen isotopes. At Vizzavona Pass in central Corsica, water samples from two catchments within a lateral distance of only a few hundred metres showed unexpected but systematic differences in their stable isotope composition. At this specific location, the direction of exposure seems to be an important factor. The differences were likely caused by isotopic enrichment during recharge in warm weather conditions in south-exposed valley flanks compared to the opposite, north-exposed valley flanks.

  2. Hydrograph separation using stable isotopes: Review and evaluation

    Science.gov (United States)

    Klaus, J.; McDonnell, J. J.

    2013-11-01

    We reviewed isotope hydrograph separation studies.We examine methods, applications, and limitations.We summarize factors that control the event/pre-event water contributions.We outline new possible research avenues in isotope hydrograph separation.

  3. Stable isotope customer list and summary of shipments - FY 1978

    Energy Technology Data Exchange (ETDEWEB)

    Davis, W.C. (comp.)

    1979-05-01

    This compilation is divided into four sections: alphabetical lists of customers, of isotopes (cross-referenced to customer numbers), and of states and countries (cross-referenced to customer numbers), and a tabulation of the shipments of each isotope. (DLC)

  4. Environmental effects on the stable carbon and oxygen isotopic ...

    African Journals Online (AJOL)

    USER

    2010-08-16

    Aug 16, 2010 ... Key words: Oxygen isotopes, carbon isotopes, Porites coral, density bands, skeletal .... isotopic ratio of CO2 gas derived from the Pee Dee Belemnite (PDB) ... water samples, a 2.0 ml of the sample was taken into a syringe and.

  5. Biogeochemistry Science and Education Part One: Using Non-Traditional Stable Isotopes as Environmental Tracers Part Two: Identifying and Measuring Undergraduate Misconceptions in Biogeochemistry

    Science.gov (United States)

    Mead, Chris

    This dissertation is presented in two sections. First, I explore two methods of using stable isotope analysis to trace environmental and biogeochemical processes. Second, I present two related studies investigating student understanding of the biogeochemical concepts that underlie part one. Fe and Hg are each biogeochemically important elements in their own way. Fe is a critical nutrient for phytoplankton, while Hg is detrimental to nearly all forms of life. Fe is often a limiting factor in marine phytoplankton growth. The largest source, by mass, of Fe to the open ocean is windblown mineral dust, but other more soluble sources are more bioavailable. To look for evidence of these non-soil dust sources of Fe to the open ocean, I measured the isotopic composition of aerosol samples collected on Bermuda. I found clear evidence in the fine size fraction of a non-soil dust Fe source, which I conclude is most likely from biomass burning. Widespread adoption of compact fluorescent lamps (CFL) has increased their importance as a source of environmental Hg. Isotope analysis would be a useful tool in quantifying this impact if the isotopic composition of Hg from CFL were known. My measurements show that CFL-Hg is isotopically fractionated, in a unique pattern, during normal operation. This fractionation is large and has a distinctive, mass-independent signature, such that CFL Hg can be uniquely identified from other sources. Misconceptions research in geology has been a very active area of research, but student thinking regarding the related field of biogeochemistry has not yet been studied in detail. From interviews with 40 undergraduates, I identified over 150 specific misconceptions. I also designed a multiple-choice survey (concept inventory) to measure understanding of these same biogeochemistry concepts. I present statistical evidence, based on the Rasch model, for the reliability and validity of this instrument. This instrument will allow teachers and researchers to

  6. Production of radioactive isotopes from stable isotopes, for nuclear medicine; A partir d`isotopes stables, production d`isotopes radioactifs pour la medecine nucleaire

    Energy Technology Data Exchange (ETDEWEB)

    Bourdoiseau, M. [CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France). Office des Rayonnements Ionisants

    1994-12-31

    Around 15 radioactive isotopes only are used for nuclear medicine diagnosis (kinetics or preferential fixation in the body); characteristics of these {gamma} and {beta}-minus emitters are presented: period, energy, production mode (isotopic filiation, fission product separation, cyclotron, reactor); details are given on applications, production mode, properties and consumption of various isotopes: Technetium 99m, Thallium 201, Iodine 123, Indium 111, Gallium 67, Rhenium 186, Erbium 169, Yttrium 90, Iron 59, Chromium 51, Krypton 81m. 1 tab.

  7. Environmental controls on stable isotope ratios in New Zealand Podocarpaceae : implications for palaeoclimate reconstruction.

    OpenAIRE

    Brett, M.J.; Baldini, J. U. L.; Gröcke, D. R.

    2014-01-01

    Stable isotope ratios of various proxies are widely used for palaeoclimate reconstruction, and it is often assumed that isotope ratios reflect vegetation abundance or type. However, very little research exists on the isotopic equilibration of extant biomes under variable environmental conditions. In this study, carbon and oxygen isotope ratios from leaves of various Podocarpaceae genera, endemic to New Zealand, are linked to environmental parameters from the Land Environments New Zealand mode...

  8. Latitudinal Trends in Stable Isotope Signatures of Northeast Atlantic Rhodoliths

    Science.gov (United States)

    Hofmann, Laurie

    2017-04-01

    Rhodoliths are free-living calcifying red algae that form extensive beds in shallow marine benthic environments (marine organisms and contribute to carbonate sediment accumulation. There is growing concern that these organisms are sensitive to global climate change, which will have important consequences for coastal productivity and stability. Despite their significance and sensitivity, their basic photosynthetic and calcification mechanisms are not well understood. The goal of this study was to determine the plasticity of dissolved inorganic carbon (DIC) uptake mechanisms of rhodoliths along a latitudinal gradient in the Northeast (NE) Atlantic using natural stable isotope signatures. The delta 13C signature of macroalgae can be used to provide an indication of the preferred inorganic carbon source (CO2 vs. HCO3-). Here we present the total and organic delta 13C signatures of NE Atlantic rhodoliths with respect to changing temperature and light along the latitudinal gradient from the Canary Islands to Spitsbergen. A decreasing trend in delta 13C signatures with increasing latitude suggests that rhodoliths rely solely on CO2 as an inorganic carbon source at mid latitudes, while those at low latitudes may be able to utilize HCO3-. Polar rhodoliths deviate from this trend, suggesting they may have unique physiological mechanisms related to inorganic carbon acquisition and assimilation, which may have important implications for calcification in an environment undergoing rapid changing ocean chemistry.

  9. Stable isotopic analysis on ancient human bones in Jiahu site

    Institute of Scientific and Technical Information of China (English)

    HU YaoWu; S.H.AMBROSE; WANG ChangSui

    2007-01-01

    Palaeodietary analysis is one of important topics in bioarchaeology field and has been paid great attention to by Chinese archaeometrists recently. Ancient human bones in Jiahu Site were analyzed by means of stable isotopes of C, N and 0.13 human bones were excluded from 28 bones for dietary reconstruction due to their unusual collagen contents, C and N contents, and C/N atomic ratios especially.δ13C(-20.37±0.53‰)in collagen of remaining samples showed that C3 food were consumed mainly, which is consistent of the archaeological findings that rice was the staple in Jiahu. According to the difference of δ15N and δ13C values in bone collagen, the samples can be classified into four clusters. The changes of δ15N values in bone collagen and δ13C values in hydroxylapatite through whole cultural phases indicated the transition from hunting to gathering and fishing to rice agriculture and animal domestication ultimately. Meanwhile, the δ18O change in hydroxylapatite showed that palaeoclimate was relatively constant during Jiahu culture.

  10. Effects of carbonate leaching on foraminifer stable isotopes ratios

    Science.gov (United States)

    Obrochta, S.; Yokoyama, Y.; Sakai, S.; Ishimura, T.

    2011-12-01

    Stable carbon and oxygen isotope ratios were measured on 125 individual epifaunal and infaunal benthic foraminifers from two discrete Holocene intervals in a shallow-water sediment core (~ 450 m) from the Timor Sea. Methane seeps are common in the area, resulting in significant precipitation of secondary calcite that is confirmed by SEM photomicrographs and has likely resulted in inconsistent downcore results. To assess the degree of removal of contaminants, individual Uvigerina peregrina were subjected to varying degrees of pretreatment prior to analysis. All foraminifers received standard cleaning with ethanol and brief sonication. A subset were further cleaned and sonicated in a dilute HCl solution (~ 0.003 M). Foraminifer tests were photographed using both reflected light and scanning electron microscopes during the course of treatment to monitor the changing degree of contaminant removal as increasingly aggressive cleaning methods were employed. Visible contamination remained on individuals not subjected to HCl treatment. The leached individuals exhibit a lower overall relative standard deviation and consistent results within morphotype groups. Based on these results, a 2% value is expected to be typical of the Holocene, though further downcore analyses are pending restoration of equipment adversely effected by the Eastern Japan 3/11 earthquake.

  11. Development of a stable isotope dilution assay for tenuazonic acid.

    Science.gov (United States)

    Asam, Stefan; Liu, Yang; Konitzer, Katharina; Rychlik, Michael

    2011-04-13

    A stable isotope dilution assay (SIDA) for the Alternaria mycotoxin tenuazonic acid was developed. Therefore, [(13)C(6),(15)N]-tenuazonic acid was synthesized from [(13)C(6),(15)N]-isoleucine by Dieckmann intramolecular cyclization after acetoacetylation with diketene. The synthesized [(13)C(6),(15)N]-tenuazonic acid was used as the internal standard for determination of tenuazonic acid in tomato products by liquid chromatography tandem mass spectrometry after derivatization with 2,4-dinitrophenylhydrazine. Method validation revealed a limit of detection of 0.1 μg/kg and a limit of quantitation of 0.3 μg/kg. Recovery was close to 100% in the range of 3-300 μg/kg. Determination of tenuazonic acid in two samples of different tomato ketchups (naturally contaminated) was achieved with a coefficient of variation of 2.3% and 4.7%. Different tomato products (n = 16) were analyzed for their content of tenuazonic acid using the developed SIDA. Values were between 15 and 195 μg/kg (tomato ketchup, n = 9), 363 and 909 μg/kg (tomato paste, n = 2), and 8 and 247 μg/kg (pureed tomatoes and comparable products, n = 5).

  12. Stable isotopic analysis on ancient human bones in Jiahu site

    Institute of Scientific and Technical Information of China (English)

    S.H.AMBROSE

    2007-01-01

    Palaeodietary analysis is one of important topics in bioarchaeology field and has been paid great at- tention to by Chinese archaeometrists recently. Ancient human bones in Jiahu Site were analyzed by means of stable isotopes of C, N and O. 13 human bones were excluded from 28 bones for dietary re- construction due to their unusual collagen contents, C and N contents, and C/N atomic ratios espe- cially. δ 13C (-20.37±0.53‰) in collagen of remaining samples showed that C3 food were consumed mainly, which is consistent of the archaeological findings that rice was the staple in Jiahu. According to the difference of δ 15N and δ 13C values in bone collagen, the samples can be classified into four clusters. The changes of δ 15N values in bone collagen and δ 13C values in hydroxylapatite through whole cultural phases indicated the transition from hunting to gathering and fishing to rice agriculture and animal domestication ultimately. Meanwhile, the δ 18O change in hydroxylapatite showed that pa- laeoclimate was relatively constant during Jiahu culture.

  13. Electric Monopole Transition Strengths in Stable Nickel Isotopes

    Science.gov (United States)

    Evitts, Lee; Garnsworthy, Adam; Kibedi, Tibor; Super-e Collaboration

    2016-09-01

    Electric monopole (E 0) transition strengths are a sensitive probe for investigating nuclear structure and shape coexistence. There is a need for E 0 transition strengths in closed shell nuclei in order to develop our understanding of the mechanisms responsible for the generation of electric monopole strength. Simultaneous detections of γ rays and internal conversion electrons must be measured in order to determine an E 0 transition strength. A series of measurements in the stable nickel isotopes were performed at the Australian National University. Excited states in 58 , 60 , 62Ni were populated via inelastic proton scattering. The CAESAR array of Compton-suppressed HPGe detectors was used to measure the (E 2 / M 1) mixing ratio of transitions from angular distributions of γ rays. The Super-e spectrometer was used to measure electron-gamma branching ratios in order to extract E 0 transition strengths for a number of Jπ ->Jπ transitions. An overview of the experiments will be presented, along with preliminary results for E 0 transition strengths between Jπ ≠ 0 states in the semi-magic nuclei, 58 , 60 , 62Ni. A comparison with the matrix elements obtained from a new microscopic model for E 0 transitions will be made. This work was supported in part by the Natural Sciences and Engineering Research Council of Canada (NSERC).

  14. Stable Carbon Isotope Record in a Palau Sclerosponge

    Science.gov (United States)

    Grottoli, A. G.

    2002-12-01

    The ratio of stable carbon isotopes (δ13C) deposited in the calcium carbonate skeleton of marine sclerosponges appears to record the carbon isotopic composition of seawater mixed-layer dissolved inorganic carbon (δ13CDIC). Thus the δ13C signature chronicled in sclerosponge skeletons offers a promising multi-century proxy record of seawater mixed-layer δ13CDIC throughout the tropics. Here, a high-resolution (0.1 mm) δ13C record for a 7.7 cm Acanthocheatetes wellsi sclerosponge from Palau (7N, 134W) is presented. At a published growth rate of 0.45 mm per year, this record spans ~s170 years beginning in July 2001 and going back to 1831. The δ13C values for a definitive 10-year A. wellsi record spanning 1989-1998 were similar to δ13C values here for the first 4.7 mm of the record providing supporting evidence for the growth rate. The sclerosponge δ13C shows a distinct Seuss Effect. At the time this abstract was submitted, the analysis of the first 16 mm of the sclerosponge revealed a significant decrease in δ13C with time [δ13C = 0.02 (distance) + 2.64, r2 = 0.73, p < 0.0001, where time is marked by distance in millimeters from the growing edge] corresponding to a decrease in δ13C of 0.076‰ per decade. For comparison, published low-frequency measurements in Australian, New Caledonian and Jamaican sclerosponges have yielded decreases in δ13C of ~s0.05 to 0.08 ‰ per decade over the past 40 years. Preliminary interpretation of the data indicates that the amount of atmospheric CO2 contributing to the seawater δ13CDIC at Palau is intermediate to Australia and Jamaica. In addition, visual examination of the δ13C record reveals regular fluctuation in δ13C that may correspond to annual variability in δ13CDIC. This research presents the first century or longer sclerosponge δ13C record from the northwester equatorial Pacific.

  15. Forensic Applications of Light-Element Stable Isotope Ratios of Ricinus communis Seeds and Ricin Preparations

    Energy Technology Data Exchange (ETDEWEB)

    Kreuzer, Helen W.; West, Jason B.; Ehleringer, James

    2013-01-01

    Seeds of the castor plant Ricinus communis, also known as castor beans, are of forensic interest because they are the source of the poison ricin. We have tested whether stable isotope ratios of castor seeds and ricin prepared by various methods can be used as a forensic signature. We collected over 300 castor seed samples from locations around the world and measured the C, N, O, and H stable isotope ratios of the whole seeds, oil, and three types of ricin preparations. Our results demonstrate that N isotope ratios can be used to correlate ricin prepared by any of these methods to source seeds. Further, stable isotope ratios distinguished >99% of crude and purified ricin protein samples in pair-wise comparison tests. Stable isotope ratios therefore constitute a valuable forensic signature for ricin preparations.

  16. Stable isotope applications in biomolecular structure and mechanisms. A meeting to bring together producers and users of stable-isotope-labeled compounds to assess current and future needs

    Energy Technology Data Exchange (ETDEWEB)

    Trewhella, J.; Cross, T.A.; Unkefer, C.J. [eds.

    1994-12-01

    Knowledge of biomolecular structure is a prerequisite for understanding biomolecular function, and stable isotopes play an increasingly important role in structure determination of biological molecules. The first Conference on Stable Isotope Applications in Biomolecular Structure and Mechanisms was held in Santa Fe, New Mexico, March 27--31, 1994. More than 120 participants from 8 countries and 44 institutions reviewed significant developments, discussed the most promising applications for stable isotopes, and addressed future needs and challenges. Participants focused on applications of stable isotopes for studies of the structure and function of proteins, peptides, RNA, and DNA. Recent advances in NMR techniques neutron scattering, EPR, and vibrational spectroscopy were highlighted in addition to the production and synthesis of labeled compounds. This volume includes invited speaker and poster presentations as well as a set of reports from discussion panels that focused on the needs of the scientific community and the potential roles of private industry, the National Stable Isotope Resource, and the National High Magnetic Field Laboratory in serving those needs. This is the leading abstract. Individual papers are processed separately for the database.

  17. The geochemistry of stable chlorine and bromine isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Eggenkamp, Hans [Onderzock and Beleving, Bussum (Netherlands)

    2014-11-01

    First book solely dedicated to the geochemistry of chlorine and bromine isotopes. Detailed description of analytical techniques, including their advantages and disadvantages. Indication of research fields where measurement of these isotopes is especially useful. This book provides detailed information on the history, analysis and applications of chlorine and bromine isotope geochemistry. Chlorine and bromine are geochemically unique as they prefer to exist as single charged negative ions. For this reason isotope fractionation reflects mostly processes that are not related to changes in the redox state and this fractionation is generally modest. The book will describe the processes that are most easily detected using these isotopes. Also isotope variations, and processes that cause them, measured in oxidised species such as perchlorates and in organic molecules will be described in this book.

  18. Late Quaternary palaeoenvironmental reconstruction from Lake Ohrid using stable isotopes

    Science.gov (United States)

    Lacey, Jack H.; Leng, Melanie J.; Francke, Alexander; Vogel, Hendrik; Zanchetta, Giovanni; Wagner, Bernd

    2016-04-01

    Lake Ohrid is a large, deep lake located on the Balkan Peninsula at the border between Macedonia and Albania, and is considered the oldest extant lake in Europe. An International Continental scientific Drilling Program (ICDP) deep drilling campaign was carried out in 2013 as part of the interdisciplinary Scientific Collaboration On Past Speciation Conditions in Lake Ohrid (SCOPSCO) project. Over 1500 m of sediment were recovered from six coring locations at the main target site in the central basin, where the maximum drill depth reached 569 m below the lake floor. Initial results indicate continuous lacustrine conditions over the past >1.2 Ma (Wagner et al., 2014). Here, we present oxygen and carbon isotope data (δ18O and δ13C) from carbonate from the upper 248 m of the SCOPSCO succession, which covers the last 640 ka, spanning marine isotope stages 15-1, according to an age model based on tephra and orbital tuning (Francke et al., 2015). Modern monitoring data show Lake Ohrid to be an evaporative system, where variations in δ18O of endogenic carbonate are primarily a function of changes in water balance, and δ13C largely reflects fluctuations in the amount of soil-derived CO2 and organic matter recycling. Our results indicate a trend from wetter to drier conditions through the Holocene, which is consistent with regional and hemispheric processes related to changes in insolation and progressive aridification. Over the last 640 ka, relatively stable climate conditions are inferred before ca. 450 ka, a transition to a wetter climate between ca. 400-250 ka, and a trend to drier climate after ca. 250 ka. Higher frequency, multi-millennial-scale oscillations observed during warm stages are most likely associated with regional climate change as a function of orbital forcing. This record is one of the most extensive and highly-resolved continental isotope records available, and emphasises the potential of Lake Ohrid as a valuable archive of long-term palaeoclimate and

  19. Trophic ecology of small yellow croaker (Larimichthys polyactis Bleeker): stable carbon and nitrogen isotope evidence

    Institute of Scientific and Technical Information of China (English)

    JI Weiwei; CHEN Xuezhong; JIANG Yazhou; LI Shengfa

    2011-01-01

    The trophic ecology of the small yellow croaker (Larimichthys polyactis) was studied using stable isotope analyses.Samples were collected from July to September 2009 and 34 individuals from eight sites were examined for stable carbon and nitrogen isotopes.Stable carbon isotope ratios (δ13C)ranged from -20.67 to -15.43,while stable nitrogen isotope ratios (δ15N) ranged 9.18-12.23.The relationship between δ13C and δ15N suggested high resource partitioning in the sampling area.Significant differences in stable isotope values among the eight sampling sites may be linked to environmental diversities involving various physical processes (such as ocean current,wind and tide) and different carbon sources.Furthermore,the stable isotope ratios may also explain the ontogenetic variability in diet and feeding,because δ13C and δ15N varied significantly with increasing body size.The findings are consistent with other studies on diet analyses in small yellow croaker.It was also demonstrated that stable isotope analysis could be used to estimate the trophic characters of small yellow croaker in feeding patterns and migrating habits.

  20. Phosphate stable oxygen isotope variability within a temperate agricultural soil.

    Science.gov (United States)

    Granger, Steven J; Harris, Paul; Peukert, Sabine; Guo, Rongrong; Tamburini, Federica; Blackwell, Martin S A; Howden, Nicholas J K; McGrath, Steve

    2017-01-01

    In this study, we conduct a spatial analysis of soil total phosphorus (TP), acid extractable phosphate (PO4) and the stable oxygen (O) isotope ratio within the PO4 molecule (δ(18)OPO4 ) from an intensively managed agricultural grassland site. Total P in the soil was found to range from 736 to 1952 mg P kg(- 1), of which between 12 and 48% was extractable using a 1 M HCl (HClPO4 ) solution with the two variables exhibiting a strong positive correlation. The δ(18)OPO4 of the extracted PO4 ranged from 17.0 to 21.6‰ with a mean of 18.8‰ (± 0.8). While the spatial variability of Total P has been researched at various scales, this is the first study to assess the variability of soil δ(18)OPO4 at a field-scale resolution. We investigate whether or not δ(18)OPO4 variability has any significant relationship with: (i) itself with respect to spatial autocorrelation effects; and (ii) HClPO4 , elevation and slope - both globally and locally. Results indicate that δ(18)OPO4 was not spatially autocorrelated; and that δ(18)OPO4 was only weakly related to HClPO4 , elevation and slope, when considering the study field as a whole. Interestingly, the latter relationships appear to vary in strength locally. In particular, the δ(18)OPO4 to HClPO4 relationship may depend on the underlying soil class and/or on different field managements that had operated across an historical north-south field division of the study field, a division that had been removed four years prior to this study.

  1. Soil phosphate stable oxygen isotopes across rainfall and bedrock gradients.

    Science.gov (United States)

    Angert, Alon; Weiner, Tal; Mazeh, Shunit; Sternberg, Marcelo

    2012-02-21

    The stable oxygen isotope compositions of soil phosphate (δ(18)O(p)) were suggested recently to be a tracer of phosphorus cycling in soils and plants. Here we present a survey of bioavailable (resin-extractable or resin-P) inorganic phosphate δ(18)O(p) across natural and experimental rainfall gradients, and across soil formed on sedimentary and igneous bedrock. In addition, we analyzed the soil HCl-extractable inorganic δ(18)O(p), which mainly represents calcium-bound inorganic phosphate. The resin-P values were in the range 14.5-21.2‰. A similar range, 15.6-21.3‰, was found for the HCl-extractable inorganic δ(18)O(p), with the exception of samples from a soil of igneous origin that show lower values, 8.2-10.9‰, which indicate that a large fraction of the inorganic phosphate in this soil is still in the form of a primary mineral. The available-P δ(18)O(p) values are considerably higher than the values we calculated for extracellular hydrolysis of organic phosphate, based on the known fractionation from lab experiments. However, these values are close to the values expected for enzymatic-mediated phosphate equilibration with soil-water. The possible processes that can explain this observation are (1) extracellular equilibration of the inorganic phosphate in the soil; (2) fractionations in the soil are different than the ones measured at the lab; (3) effect of fractionation during uptake; and (4) a flux of intercellular-equilibrated inorganic phosphate from the soil microbiota, which is considerably larger than the flux of hydrolyzed organic-P.

  2. The stable Cr isotopic compositions of chondrites and silicate planetary reservoirs

    Science.gov (United States)

    Schoenberg, Ronny; Merdian, Alexandra; Holmden, Chris; Kleinhanns, Ilka C.; Haßler, Kathrin; Wille, Martin; Reitter, Elmar

    2016-06-01

    The depletion of chromium in Earth's mantle (∼2700 ppm) in comparison to chondrites (∼4400 ppm) indicates significant incorporation of chromium into the core during our planet's metal-silicate differentiation, assuming that there was no significant escape of the moderately volatile element chromium during the accretionary phase of Earth. Stable Cr isotope compositions - expressed as the ‰-difference in 53Cr/52Cr from the terrestrial reference material SRM979 (δ53/52CrSRM979 values) - of planetary silicate reservoirs might thus yield information about the conditions of planetary metal segregation processes when compared to chondrites. The stable Cr isotopic compositions of 7 carbonaceous chondrites, 11 ordinary chondrites, 5 HED achondrites and 2 martian meteorites determined by a double spike MC-ICP-MS method are within uncertainties indistinguishable from each other and from the previously determined δ53/52CrSRM979 value of -0.124 ± 0.101‰ for the igneous silicate Earth. Extensive quality tests support the accuracy of the stable Cr isotope determinations of various meteorites and terrestrial silicates reported here. The uniformity in stable Cr isotope compositions of samples from planetary silicate mantles and undifferentiated meteorites indicates that metal-silicate differentiation of Earth, Mars and the HED parent body did not cause measurable stable Cr isotope fractionation between these two reservoirs. Our results also imply that the accretionary disc, at least in the inner solar system, was homogeneous in its stable Cr isotopic composition and that potential volatility loss of chromium during accretion of the terrestrial planets was not accompanied by measurable stable isotopic fractionation. Small but reproducible variations in δ53/52CrSRM979 values of terrestrial magmatic rocks point to natural stable Cr isotope variations within Earth's silicate reservoirs. Further and more detailed studies are required to investigate whether silicate

  3. Stable isotopes and mercury in a model estuarine fish: Multibasin comparisons with water quality, community structure, and available prey base

    Energy Technology Data Exchange (ETDEWEB)

    Adams, Douglas H., E-mail: Doug.Adams@MyFWC.com; Paperno, Richard

    2012-01-01

    Stable-isotope ratios ({delta}{sup 13}C and {delta}{sup 15}N) and mercury in a model predator, and associated prey community assessments were used to make inferences regarding food web relationships and how these relationships are influenced by habitat variability and anthropogenic factors. Although interconnected, the three major basins of the Indian River Lagoon system on the Atlantic coast of Florida comprise noticeably different available habitat types with spatially distinct faunal communities and available prey for spotted seatrout, Cynoscion nebulosus, a model predatory fish species. Water quality, degree of urbanization, human population density, and levels of nitrogen enrichment clearly differ between these representative estuarine basins. The differences can influence feeding ecology and therefore result in different mercury concentrations and different stable-isotope signatures of spotted seatrout between basins. Mercury concentrations in spotted seatrout were greatest in Mosquito Lagoon (ML) and least in the Indian River Lagoon proper (IRL), although concentrations were low for all basins. Spotted seatrout from IRL were carbon-depleted and nitrogen-enriched compared with those from the other basins; this suggests either that the fish's primary source of carbon in IRL is an algae- or phytoplankton-based food web or that the pathway through the food web is shorter there. The {delta}{sup 15}N values of IRL spotted seatrout were greater than those in the Banana River Lagoon or ML, suggesting slightly different trophic positioning of fish in these basins. The greater {delta}{sup 15}N values in IRL spotted seatrout may also reflect the greater human population density and resultant anthropogenic inputs (e.g., observed higher total nitrogen levels) in IRL compared with the other more pristine basins examined. Understanding species' responses to broad-scale habitat heterogeneity in estuaries and knowing basin-specific differences in stable isotopes

  4. Relationship between self-reported fish and shellfish consumption, carbon and nitrogen stable isotope values and total mercury concentrations in pregnant women (II from Baja California Sur, Mexico

    Directory of Open Access Journals (Sweden)

    Rebecca Bentzen

    2014-01-01

    Full Text Available Seafood is a valuable source of nutrients important for fetal development. However, seafood consumption is the main route of exposure to monomethyl mercury (MeHg+ for humans. MeHg+ is highly bioavailable and potentially adversely affects fetal neurodevelopment. MeHg+ exposure from fish consumption varies significantly by age and trophic level of fish consumed as well as the frequency and amount of fish consumed. This study investigates total Hg concentrations ([THg] in hair segments of pregnant Mexican women in relation to (1 self-reported frequency of fish and shellfish consumption, (2 maternal trophic level and marine diet contributions, determined using hair carbon (C and nitrogen (N stable isotopes, and (3 relates [THg] to various hair advisory thresholds. We also examined whether variation in C and N isotope values is explained by self-reported frequency of fish and shellfish consumption. A significant proportion of hair samples had [THg] higher than suggested agency thresholds and, for women within the range of the various advisory thresholds (1–20 μg g−1, the specific statistic used and threshold applied are important considerations for assessing and communicating risk. Individuals enriched in 15N (δ15N values had higher [THg] as did individuals that reported consuming fish and shellfish more frequently, suggesting that variation in [THg] can be explained by both consumer reported diet and diet as determined by C and N stable isotope assessment. However, at higher reported fish consumption levels the trophic level is maintained while [THg] is paradoxically lower. This suggests that THg exposure and assimilation are more complicated in higher fish frequency consumption categories. [THg] is more variable at the higher concentrations, possibly indicating some exposure to non-dietary Hg, heritable variations affecting Hg toxicodynamics, and BMI and tobacco exposure factors as outlined in our companion paper.

  5. Stable isotope utilization for research on human nutrition; L`utilisation d`isotopes stables pour la recherche en nutrition humaine

    Energy Technology Data Exchange (ETDEWEB)

    Desjeux, J.F. [Conservatoire National des Arts et Metiers (CNAM), 75 - Paris (France)

    1994-12-31

    In the framework of nutritional molecule metabolism research, this paper presents the various stable isotopes used as labels for biological molecules, the reasons for their application in human nutritional study (mainly because of their non toxicity) and the various analysis methods (isotope ratio mass spectrometry, coupled gaseous chromatography and mass spectrometry, nuclear magnetic resonance). Several application examples in nutrition research are then discussed: metabolic conversion measurement for a molecule into its different metabolites, energetic losses. 23 refs.

  6. Spatiotemporal variation of stable isotopic composition in precipitation

    DEFF Research Database (Denmark)

    Müller, Sascha; Stumpp, Christine; Sørensen, Jens Havskov

    2017-01-01

    gradient and predominant westerly winds. Data showed the local meteoric water line for this region is expressed by the equation δ2H = 7.4δ18O + 5.4‰. A significant trend correlating enriched isotopic values to humidities around 70% during dry season and more depleted isotopic values to humidities around 90...

  7. Stable Isotope Measurements of Carbon Dioxide, Methane, and Hydrogen Sulfide Gas Using Frequency Modulation Spectroscopy

    Science.gov (United States)

    Nowak-Lovato, K.

    2014-12-01

    Seepage from enhanced oil recovery, carbon storage, and natural gas sites can emit trace gases such as carbon dioxide, methane, and hydrogen sulfide. Trace gas emission at these locations demonstrate unique light stable isotope signatures that provide information to enable source identification of the material. Light stable isotope detection through surface monitoring, offers the ability to distinguish between trace gases emitted from sources such as, biological (fertilizers and wastes), mineral (coal or seams), or liquid organic systems (oil and gas reservoirs). To make light stable isotope measurements, we employ the ultra-sensitive technique, frequency modulation spectroscopy (FMS). FMS is an absorption technique with sensitivity enhancements approximately 100-1000x more than standard absorption spectroscopy with the advantage of providing stable isotope signature information. We have developed an integrated in situ (point source) system that measures carbon dioxide, methane and hydrogen sulfide with isotopic resolution and enhanced sensitivity. The in situ instrument involves the continuous collection of air and records the stable isotope ratio for the gas being detected. We have included in-line flask collection points to obtain gas samples for validation of isotopic concentrations using our in-house isotope ratio mass spectroscopy (IRMS). We present calibration curves for each species addressed above to demonstrate the sensitivity and accuracy of the system. We also show field deployment data demonstrating the capabilities of the system in making live dynamic measurements from an active source.

  8. Stable isotopic signature of Australian monsoon controlled by regional convection

    Science.gov (United States)

    Zwart, C.; Munksgaard, N. C.; Kurita, N.; Bird, M. I.

    2016-11-01

    The aim of this study was to identify the main meteorological drivers of rainfall isotopic variation in north Australia in order to improve the interpretation of isotopic proxy records in this region. An intense monitoring program was conducted during two monsoonal events that showed significant and systematic isotopic change over time. The results showed a close link between isotopic variation in precipitation and variability in monsoon conditions, associated with the presence of large convective envelopes propagating through the study site. The largest negative amplitudes in the isotopic signal were observed when eastward and westward moving precipitation systems within the convective envelope merged over the measurement site. This suggests that the amplitude of the isotopic signal is related to the size and activity of the convective envelope. The strong correlation between rainfall isotopic variation, regional outgoing longwave radiation and regional rainfall amount supports this conclusion. This is further strengthened by the strong relationship between isotopic variation and the integrated rainfall history of air masses prior to arriving at the measurement locations. A local amount effect was not significant and these findings support the interpretation of δ18O as proxy for regional climatic conditions rather than local rainfall amount. Meteorological parameters that characterize intra-seasonal variability of monsoon conditions were also found to be strongly linked to inter-seasonal variability of the monthly based δ18O values in the Global Network of Isotopes in Precipitation (GNIP) database. This leads to the conclusion that information about the Australian monsoon variability can likely be inferred from the isotopic proxy record in North Australia on short (intra seasonal) and long (inter seasonal or longer) timescales.

  9. Stable isotopic variation in tropical forest plants for applications in primatology.

    Science.gov (United States)

    Blumenthal, Scott A; Rothman, Jessica M; Chritz, Kendra L; Cerling, Thure E

    2016-10-01

    Stable isotope analysis is a promising tool for investigating primate ecology although nuanced ecological applications remain challenging, in part due to the complex nature of isotopic variability in plant-animal systems. The aim of this study is to investigate sources of carbon and nitrogen isotopic variation at the base of primate food webs that reflect aspects of primate ecology. The majority of primates inhabit tropical forest ecosystems, which are dominated by C3 vegetation. We used stable isotope ratios in plants from Kibale National Park, Uganda, a well-studied closed-canopy tropical forest, to investigate sources of isotopic variation among C3 plants related to canopy stratification, leaf age, and plant part. Unpredictably, our results demonstrate that vertical stratification within the canopy does not explain carbon or nitrogen isotopic variation in leaves. Leaf age can be a significant source of isotopic variation, although the direction and magnitude of this difference is not consistent across tree species. Some plant parts are clearly differentiated in carbon and nitrogen isotopic composition, particularly leaves compared to non-photosynthetic parts such as reproductive parts and woody stem parts. Overall, variation in the isotopic composition of floral communities, plant species, and plant parts demonstrates that stable isotope studies must include analysis of local plant species and parts consumed by the primates under study from within the study area. Am. J. Primatol. 78:1041-1054, 2016. © 2015 Wiley Periodicals, Inc.

  10. Enzymatic synthesis and RNA interference of nucleosides incorporating stable isotopes into a base moiety.

    Science.gov (United States)

    Hatano, Akihiko; Shiraishi, Mitsuya; Terado, Nanae; Tanabe, Atsuhiro; Fukuda, Kenji

    2015-10-15

    Thymidine phosphorylase was used to catalyze the conversion of thymidine (or methyluridine) and uracil incorporating stable isotopes to deoxyuridine (or uridine) with the uracil base incorporating the stable isotope. These base-exchange reactions proceeded with high conversion rates (75-96%), and the isolated yields were also good (64-87%). The masses of all synthetic compounds incorporating stable isotopes were identical to the theoretical molecular weights via EIMS. (13)C NMR spectra showed spin-spin coupling between (13)C and (15)N in the synthetic compounds, and the signals were split, further proving incorporation of the isotopes into the compounds. The RNA interference effects of this siRNA with uridine incorporating stable isotopes were also investigated. A 25mer siRNA had a strong knockdown effect on the MARCKS protein. The insertion position and number of uridine moieties incorporating stable isotopes introduced into the siRNA had no influence on the silencing of the target protein. This incorporation of stable isotopes into RNA and DNA has the potential to function as a chemically benign tracer in cells.

  11. Mercury bioaccumulation in estuarine fishes: Novel insights from sulfur stable isotopes

    Science.gov (United States)

    Willacker, James J.; Eagles-Smith, Collin A.; Ackerman, Joshua T.

    2017-01-01

    Estuaries are transitional habitats characterized by complex biogeochemical and ecological gradients that result in substantial variation in fish total mercury concentrations (THg). We leveraged these gradients and used carbon (δ13C), nitrogen (δ15N), and sulfur (δ34S) stable isotopes to examine the ecological and biogeochemical processes underlying THg bioaccumulation in fishes from the San Francisco Bay Estuary. We employed a tiered approach that first examined processes influencing variation in fish THg among wetlands, and subsequently examined the roles of habitat and within-wetland processes in generating larger-scale patterns in fish THg. We found that δ34S, an indicator of sulfate reduction and habitat specific-foraging, was correlated with fish THg at all three spatial scales. Over the observed ranges of δ34S, THg concentrations in fish increased by up to 860% within wetlands, 560% among wetlands, and 291% within specific impounded wetland habitats. In contrast, δ13C and δ15N were not correlated with THg among wetlands and were only important in low salinity impounded wetlands, possibly reflecting more diverse food webs in this habitat. Together, our results highlight the key roles of sulfur biogeochemistry and ecology in influencing estuarine fish THg, as well as the importance of fish ecology and habitat in modulating the relationships between biogeochemical processes and Hg bioaccumulation.

  12. Mercury Bioaccumulation in Estuarine Fishes: Novel Insights from Sulfur Stable Isotopes.

    Science.gov (United States)

    Willacker, James J; Eagles-Smith, Collin A; Ackerman, Joshua T

    2017-02-21

    Estuaries are transitional habitats characterized by complex biogeochemical and ecological gradients that result in substantial variation in fish total mercury concentrations (THg). We leveraged these gradients and used carbon (δ(13)C), nitrogen (δ(15)N), and sulfur (δ(34)S) stable isotopes to examine the ecological and biogeochemical processes underlying THg bioaccumulation in fishes from the San Francisco Bay Estuary. We employed a tiered approach that first examined processes influencing variation in fish THg among wetlands, and subsequently examined the roles of habitat and within-wetland processes in generating larger-scale patterns in fish THg. We found that δ(34)S, an indicator of sulfate reduction and habitat specific-foraging, was correlated with fish THg at all three spatial scales. Over the observed ranges of δ(34)S, THg concentrations in fish increased by up to 860% within wetlands, 560% among wetlands, and 291% within specific impounded wetland habitats. In contrast, δ(13)C and δ(15)N were not correlated with THg among wetlands and were only important in low salinity impounded wetlands, possibly reflecting more diverse food webs in this habitat. Together, our results highlight the key roles of sulfur biogeochemistry and ecology in influencing estuarine fish THg, as well as the importance of fish ecology and habitat in modulating the relationships between biogeochemical processes and Hg bioaccumulation.

  13. Utilization of stable isotopes for characterizing an underground gas generator; Utilisation des isotopes stables pour caracteriser un gazogene souterrain

    Energy Technology Data Exchange (ETDEWEB)

    Pirard, J.P.; Antenucci, D.; Renard, X. [Liege Univ. (Belgium); Letolle, R. [Paris-6 Univ., 75 (France)

    1994-12-31

    The principles of isotopic exchange and isotope ratio result interpretation are first reviewed; then, in the framework of an underground coal gasification project in Belgium, experiments and modelling of the underground gas generator have been carried out: isotopic abundances of carbon, hydrogen and oxygen have been measured in the gasifying agent (O{sub 2}, H{sub 2}O) and in the effluent (CO{sub 2}, CO, H{sub 2}, H{sub 2}O, CH{sub 4}, O{sub 2}, heavy oils and various organic and mineral substances). Gasification kinetics and temperatures have been evaluated and isotope application to thermometry is discussed. 1 fig., 9 refs.

  14. Stable isotope signatures of gases liberated from fluid inclusions in bedrock at Olkiluoto

    Energy Technology Data Exchange (ETDEWEB)

    Eichinger, F. (Hydroisotop GmbH, Schweitenkirchen (DE)); Meier, D.; Haemmerli, J.; Diamond, L. (Bern Univ. (CH), RWI, Institute of Geological Sciences)

    2010-12-15

    Fluid inclusions in quartzes of the Olkiluoto bedrock contain gaseous N{sub 2}, CO{sub 2}, H{sub 2}, CH{sub 4}, and higher hydrocarbons in varying proportions. Stable carbon and hydrogen isotope signatures of the gas phases give valuable information on their origin and the formation conditions. In previous studies, a method to liberate and quantify the gases trapped in fluid inclusions was developed. It allowed determining the carbon isotope signatures of liberated CO{sub 2}, CH{sub 4} and higher hydrocarbons (HHC), but no hydrogen isotope data were acquired. The method was advanced and, in this study, also stable hydrogen isotopes of CH{sub 4} and H{sub 2} liberated from fluid inclusions could be analysed. The stable carbon signatures of methane and higher hydrocarbons, as well as the hydrogen isotope signatures of methane indicate a predominant thermogenic provenance for those gases. (orig.)

  15. Continuous in situ measurements of stable isotopes in liquid water

    Science.gov (United States)

    Herbstritt, Barbara; Gralher, Benjamin; Weiler, Markus

    2012-03-01

    We developed a method to measure in situ the isotopic composition of liquid water with minimal supervision and, most important, with a temporal resolution of less than a minute. For this purpose a microporous hydrophobic membrane contactor (Membrana) was combined with an isotope laser spectrometer (Picarro). The contactor, originally designed for degassing liquids, was used with N2 as a carrier gas in order to transform a small fraction of liquid water to water vapor. The generated water vapor was then analyzed continuously by the Picarro analyzer. To prove the membrane's applicability, we determined the specific isotope fractionation factor for the phase change through the contactor's membrane across an extended temperature range (8°C-21°C) and with different waters of known isotopic compositions. This fractionation factor is needed to subsequently derive the liquid water isotope ratio from the measured water vapor isotope ratios. The system was tested with a soil column experiment, where the isotope values derived with the new method corresponded well (R2 = 0.998 for δ18O and R2 = 0.997 for δ2H) with those of liquid water samples taken simultaneously and analyzed with a conventional method (cavity ring-down spectroscopy). The new method supersedes taking liquid samples and employs only relatively cheap and readily available components. This makes it a relatively inexpensive, fast, user-friendly, and easily reproducible method. It can be applied in both the field and laboratory wherever a water vapor isotope analyzer can be run and whenever real-time isotope data of liquid water are required at high temporal resolution.

  16. Priming effects of biochar elucidated using stable isotope techniques.

    Science.gov (United States)

    Hood-Nowotny, R.; Vanlauwe, B.

    2012-04-01

    Organic residues are routinely used in tropical agricultural systems; as mulches to reduce water losses and for their fertiliser value. The addition of high N content organic residues to soils has been promoted in tropical countries as a means to achieve sustainable intensification of tropical farming systems and increasing soil organic matter status on infertile low income farms. Improving the nutrient release from these materials could have positive feedback effects in terms of improved food security and increased organic matter return to the soil through improved crop yields. Unfortunately the fertiliser value of most organic residues is such that only 10 -20% of the available nitrogen in the residue is mineralised to plant available nitrogen and taken up by the plant in the first cropping year, dropping to less than 2% in the subsequent years; thus having marginal overall impact on crop yields. Improving the fertiliser benefit of residues by combining them with the biochar addition could lead to significant increases in crop yields, an immediately tangible benefit for farmers. The addition of charcoal in boreal forest systems has been shown to increase the rate of soil organic matter decomposition, suggesting there is a priming effect of a biochar analogue on organic matter decomposition. The priming effect is the increase in soil organic matter (SOM) decomposition rate after the addition of fresh organic matter or other compounds to soil. The implication is that is biochar if addition leads to the loss of native SOM it negates the carbon benefit of adding biochar to soil. However there could also be potential benefits of priming effects of biochar under specific circumstances, for example if biochar addition leads to the priming of freshly added organic matter breakdown it may in turn improve nutrient synchronisation and overall nutrient use efficiency. We conducted a series of experiments conducted in Kenya and Austria using stable isotope tools to look at

  17. Metal Stable Isotope Tagging: Renaissance of Radioimmunoassay for Multiplex and Absolute Quantification of Biomolecules.

    Science.gov (United States)

    Liu, Rui; Zhang, Shixi; Wei, Chao; Xing, Zhi; Zhang, Sichun; Zhang, Xinrong

    2016-05-17

    The unambiguous quantification of biomolecules is of great significance in fundamental biological research as well as practical clinical diagnosis. Due to the lack of a detectable moiety, the direct and highly sensitive quantification of biomolecules is often a "mission impossible". Consequently, tagging strategies to introduce detectable moieties for labeling target biomolecules were invented, which had a long and significant impact on studies of biomolecules in the past decades. For instance, immunoassays have been developed with radioisotope tagging by Yalow and Berson in the late 1950s. The later languishment of this technology can be almost exclusively ascribed to the use of radioactive isotopes, which led to the development of nonradioactive tagging strategy-based assays such as enzyme-linked immunosorbent assay, fluorescent immunoassay, and chemiluminescent and electrochemiluminescent immunoassay. Despite great success, these strategies suffered from drawbacks such as limited spectral window capacity for multiplex detection and inability to provide absolute quantification of biomolecules. After recalling the sequences of tagging strategies, an apparent question is why not use stable isotopes from the start? A reasonable explanation is the lack of reliable means for accurate and precise quantification of stable isotopes at that time. The situation has changed greatly at present, since several atomic mass spectrometric measures for metal stable isotopes have been developed. Among the newly developed techniques, inductively coupled plasma mass spectrometry is an ideal technique to determine metal stable isotope-tagged biomolecules, for its high sensitivity, wide dynamic linear range, and more importantly multiplex and absolute quantification ability. Since the first published report by our group, metal stable isotope tagging has become a revolutionary technique and gained great success in biomolecule quantification. An exciting research highlight in this area

  18. Use of stable isotope analysis to determine of the timing of ontogenic habitat shifts

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — SAIP funding for stable isotope research was provided in FY11 and FY13; the FY11 funding was for loggerhead turtles (described below) as opposed to green turtles in...

  19. Alaska Northern Fur Seal Foraging Habitat Model Stable Isotope Data, 2006-2008

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data sets were used by Zeppelin et al. (2015) to model northern fur seal foraging habitats based on stable isotope values measured in plasma and red blood...

  20. Chromium stable isotope fractionation in modern biogeochemical cycling

    DEFF Research Database (Denmark)

    Paulukat, Cora Stefanie

    oxygen in the Earth’s atmosphere. Oxidative rock weathering on land induces oxidation of immobile Cr(III) to mobile Cr(VI). Isotopically relatively heavy Cr(VI) is released to runoff, and transported by rivers to the oceans, where it is incorporated into chemical sediments and carbonate shells...... laterite soils from India, formed on ultramafic rocks, indicates extensive leaching of isotopically heavy Cr(VI). Transferring this knowledge to ancient weathering profiles, negatively fractionated Cr is clear evidence for the presence of free oxygen in the atmosphere. The second part demonstrates...... that the positively fractionated Cr from the catchment area is preserved during its riverine transport to the sea. A global compilation of surface seawater Cr-isotope compositions shows a heterogeneous Cr-isotope distribution in the oceans, indicating that the signal is influenced by local factors as reduction...

  1. Stable water isotopes suggest sub-canopy water recycling in a northern forested catchment

    Science.gov (United States)

    Mark B. Green; Bethany K. Laursen; John L. Campbell; Kevin J. McGuire; Eric P. Kelsey

    2015-01-01

    Stable water isotopes provide a means of tracing many hydrologic processes, including poorly understood dynamics like soil water interactions with the atmosphere. We present a four-year dataset of biweekly water isotope samples from eight fluxes and stores in a headwater catchment at the Hubbard Brook Experimental Forest, New Hampshire, USA. We use Dansgaard's...

  2. Stable isotope ratio method for the characterisation of the poultry house environment.

    Science.gov (United States)

    Skipitytė, Raminta; Mašalaitė, Agnė; Garbaras, Andrius; Mickienė, Rūta; Ragažinskienė, Ona; Baliukonienė, Violeta; Bakutis, Bronius; Šiugždaitė, Jūratė; Petkevičius, Saulius; Maruška, Audrius Sigitas; Remeikis, Vidmantas

    2017-06-01

    Stable isotope analysis was applied to describe the poultry house environment. The poultry house indoor environment was selected for this study due to the relevant health problems in animals and their caretakers. Air quality parameters including temperature, relative humidity, airflow rate, NH3, CO2 and total suspended particles, as well as mean levels of total airborne bacteria and fungi count, were measured. Carbon isotope ratios ((13)C/(12)C) were obtained in size-segregated aerosol particles. The carbon ((13)C/(12)C) and nitrogen ((15)N/(14)N) isotope ratios were measured in feed, litter, scrapings from the ventilation system, feathers and eggs. Additionally, the distribution of δ(13)C and δ(15)N values in different tissues of the chicken was examined. The airborne bacteria and fungi extracted from the air filters collected from poultry farms were grown in the laboratory in media with known isotope values and measured for stable isotope ratios. Analysis of isotope fractionation between microorganisms and their media indicated the applicability of stable isotope analysis in bulk samples for the identification of source material. The analysed examples imply that stable isotope analysis can be used to examine the indoor environment along with its biology and ecology, and serve as an informative bioanalytical tool.

  3. Endogenous surfactant metabolism in critically ill infants measured with stable isotope labeled fatty acids

    NARCIS (Netherlands)

    Cogo, PE; Carnielli, VP; Bunt, JEH; Badon, T; Giordano, G; Zacchello, F; Sauer, PJJ; Zimmermann, LJI

    1999-01-01

    Little is known about endogenous surfactant metabolism in infants, because radioactive isotopes used for this purpose in animals cannot be used in humans. We developed a novel and safe method to measure the endogenous surfactant kinetics in vivo in humans by using stable isotope labeled fatty acids.

  4. Stable Isotope Studies of Crop Carbon and Water Relations: A Review

    Institute of Scientific and Technical Information of China (English)

    ZHANG Cong-zhi; ZHANG Jia-bao; ZHAO Bing-zi; ZHANG Hui; HUANG Ping

    2009-01-01

    Crop carbon and water relations research is important in the studies of water saving agriculture,breeding program,and energy and material cycles in soil plant atmosphere continuum (SPAC).The purpose of this paper is to review the current state of knowledge on stable isotopes of carbon,oxygen,and hydrogen in the research of crop carbon and water relations,such as carbon isotope discrimination (△13C) during carbon fixation process by photosynthesis,application of △13C in crop water use efficiency (WUE) and breeding programs,oxygen isotope enrichment during leaf water transpiration,CO2 fixation by photosynthesis and release by respiration,application of hydrogen isotope composition (δD) and oxygen isotope composition (δ18O) for determination of water source used by a crop,stable isotope coupling Keeling plot for investigating the carbon and water flux in ecosystem,energy and material cycle in SPAC and correlative integrative models on stable isotope.These aspects contain most of the stable isotope researches on crop carbon and water relations which have been widely explored internationally while less referred in China.Based on the reviewed literatures,some needs for future research are suggested.

  5. Stable isotope separation in calutrons: Forty years of production and distribution

    Energy Technology Data Exchange (ETDEWEB)

    Bell, W.A.; Tracy, J.G.

    1987-11-01

    The stable isotope separation program, established in 1945, has operated continually to provide enriched stable isotopes and selected radioactive isotopes, including the actinides, for use in research, medicine, and industrial applications. This report summarizes the first forty years of effort in the production and distribution of stable isotopes. Evolution of the program along with the research and development, chemical processing, and production efforts are highlighted. A total of 3.86 million separator hours has been utilized to separate 235 isotopes of 56 elements. Relative effort expended toward processing each of these elements is shown. Collection rates (mg/separator h), which vary by a factor of 20,000 from the highest to the lowest (/sup 205/Tl to /sup 46/Ca), and the attainable isotopic purity for each isotope are presented. Policies related to isotope pricing, isotope distribution, and support for the enrichment program are discussed. Changes in government funding, coupled with large variations in sales revenue, have resulted in 7-fold perturbations in production levels.

  6. Present status and strategic plan for the stable isotope reference materials at the IAEA.

    Science.gov (United States)

    Assonov, Sergey; Groening, Manfred

    2016-04-01

    The presentation will give the overview of the stable isotope reference materials (SI-RMs) under distribution by the IAEA, its stable isotope laboratory and capacities related to material testing & production as well as future plans. Historically, most of the IAEA reference materials were produced and made available via collaborations with expert stable isotope laboratories worldwide. The IAEA plans include several directions as follows: • Maintaining the scale-defining SI-RMs at the highest level and introducing adequate replacements when needed; • Monitoring existing SI-RMs for any potential alteration(s) and of isotopic values assigned; • Identifying and then addressing the needs for new SI-RMs, with the priority to address the most critical applications (environmental and climate related applications, human health, food safety studies) and newly emerging analytical isotope techniques; • Performing all measurements aimed for characterisation of new SI-RMs and the corresponding uncertainty evaluation in accordance to the latest metrological concepts; • Promoting metrological approaches on traceability and uncertainty evaluation in every day practice of stable isotope measurements; • Expanding the IAEA capacities for SI-RMs by (i) planning a renewed laboratory at IAEA; (ii) enlarging collaboration with expert laboratories aimed to help IAEA in production and characterisation of new SI-RMs. These major directions will help to address the increasing demand for Stable Isotope Reference Materials.

  7. Automated product recovery in a Hg-196 photochemical isotope separation process

    Science.gov (United States)

    Grossman, M.W.; Speer, R.

    1992-07-21

    A method of removing deposited product from a photochemical reactor used in the enrichment of [sup 196]Hg has been developed and shown to be effective for rapid re-cycling of the reactor system. Unlike previous methods relatively low temperatures are used in a gas and vapor phase process of removal. Importantly, the recovery process is understood in a quantitative manner so that scaling design to larger capacity systems can be easily carried out. 2 figs.

  8. Contamination risk of stable isotope samples during milling.

    Science.gov (United States)

    Isaac-Renton, M; Schneider, L; Treydte, K

    2016-07-15

    Isotope analysis of wood is an important tool in dendrochronology and ecophysiology. Prior to mass spectrometry analysis, wood must be homogenized, and a convenient method involves a ball mill capable of milling samples directly in sample tubes. However, sample-tube plastic can contaminate wood during milling, which could lead to biological misinterpretations. We tested possible contamination of whole wood and cellulose samples during ball-mill homogenization for carbon and oxygen isotope measurements. We used a multi-factorial design with two/three steel milling balls, two sample amounts (10 mg, 40 mg), and two milling times (5 min, 10 min). We further analyzed abrasion by milling empty tubes, and measured the isotope ratios of pure contaminants. A strong risk exists for carbon isotope bias through plastic contamination: the δ(13) C value of polypropylene deviated from the control by -6.77‰. Small fibers from PTFE filter bags used during cellulose extraction also present a risk as the δ(13) C value of this plastic deviated by -5.02‰. Low sample amounts (10 mg) showed highest contamination due to increased abrasion during milling (-1.34‰), which is further concentrated by cellulose extraction (-3.38‰). Oxygen isotope measurements were unaffected. A ball mill can be used to homogenize samples within test tubes prior to oxygen isotope analysis, but not prior to carbon or radiocarbon isotope analysis. There is still a need for a fast, simple and contamination-free sample preparation procedure. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  9. Stable water isotope and surface heat flux simulation using ISOLSM: Evaluation against in-situ measurements

    KAUST Repository

    Cai, Mick Y.

    2015-04-01

    The stable isotopes of water are useful tracers of water sources and hydrological processes. Stable water isotope-enabled land surface modeling is a relatively new approach for characterizing the hydrological cycle, providing spatial and temporal variability for a number of hydrological processes. At the land surface, the integration of stable water isotopes with other meteorological measurements can assist in constraining surface heat flux estimates and discriminate between evaporation (E) and transpiration (T). However, research in this area has traditionally been limited by a lack of continuous in-situ isotopic observations. Here, the National Centre for Atmospheric Research stable isotope-enabled Land Surface Model (ISOLSM) is used to simulate the water and energy fluxes and stable water isotope variations. The model was run for a period of one month with meteorological data collected from a coastal sub-tropical site near Sydney, Australia. The modeled energy fluxes (latent heat and sensible heat) agreed reasonably well with eddy covariance observations, indicating that ISOLSM has the capacity to reproduce observed flux behavior. Comparison of modeled isotopic compositions of evapotranspiration (ET) against in-situ Fourier Transform Infrared spectroscopy (FTIR) measured bulk water vapor isotopic data (10. m above the ground), however, showed differences in magnitude and temporal patterns. The disparity is due to a small contribution from local ET fluxes to atmospheric boundary layer water vapor (~1% based on calculations using ideal gas law) relative to that advected from the ocean for this particular site. Using ISOLSM simulation, the ET was partitioned into E and T with 70% being T. We also identified that soil water from different soil layers affected T and E differently based on the simulated soil isotopic patterns, which reflects the internal working of ISOLSM. These results highlighted the capacity of using the isotope-enabled models to discriminate

  10. Utilization of stable isotopes in pharmacology and living matter sciences; Emploi des isotopes stables en pharmacologie et dans les sciences de la matiere vivante

    Energy Technology Data Exchange (ETDEWEB)

    Brazier, J.L. [Institut des Sciences Pharmaceutiques et Biologiques, 69 -Lyon (France)

    1994-12-31

    A survey of the various application fields of stable isotopes in the pharmacological and biomedical domains is presented: utilization of labelled molecules as real tracers (metabolism studies with chromatography and mass spectrometry, studies in bio-pharmacy, bio-availability and bio-equivalence for comparison of medicaments forms, use of stable isotope pulsed doses for medicament kinetics study); methods using voluntary induced isotopic effects in a biologic system; utilization of the carbon {sup 13}C/{sup 12}C ratio and its evolution with time in a living system for in vitro, ex vivo, in vivo studies on man and animals: metabolic tests, dysabsorption tests, bacterial proliferation and infection tests, enzymatic induction and inhibition determination tests.

  11. Stable isotopes as a tool to apportion atmospheric iron.

    Science.gov (United States)

    Majestic, Brian J; Anbar, Ariel D; Herckes, Pierre

    2009-06-15

    Identification of atmospheric iron is a key parameter to understanding the source of iron in urban and remote areas. Atmospheric deposition of desert dust, which also can include an anthropogenic component, is a primary nutrient source for most of the open ocean. To better assess particulate matter (PM) sources specific to iron, we measured the iron isotopic composition of aerosols in two size fractions: PM with aerodynamic diameters less than 2.5 microm and less than 10 microm (PM2.5 and PM10, respectively). Using colocated samplers, atmospheric aerosol samples were collected in the U.S. desert Southwest at a mixed suburban/agricultural site near Phoenix, AZ. The measurements are presented as delta56Fe relative to the IRMM-014 (Institute for Reference Materials and Measurements) standard. Using multiple collector inductively coupled plasma mass spectrometry, we found differences in iron isotopic composition within the PM10 aerosol. Half of the PM10 samples had an iron isotopic signature similar to crustal material (+0.03 per thousand), which implicates wind-blown soil-dust as the primary source. The other PM10 samples showed a lighter iron isotopic composition, centered at -0.18 per thousand. Further analysis showed thatthe lighter iron was associated with winds originating from the southwest. This strongly suggests that there is a different PM10 source in this direction, with a distinct iron isotopic composition. The iron in the PM2.5 samples was usually substantially lighter than the corresponding PM10 samples, which is consistent with coarse and fine particles having different sources, again with distinctively different isotopic compositions. The magnitude of the iron isotopic difference between the PM10 and the PM2.5 size fractions (delta56Fe(PM10) - delta56Fe(PM2.5)) correlated with the PM2.5 concentrations of elements known to be emitted from industrial sources (Pb, Cd, As, V, and Cr). This observation implies that the isotopically light iron is created or

  12. Titanium stable isotopic variations in chondrites, achondrites and lunar rocks

    Science.gov (United States)

    Greber, Nicolas D.; Dauphas, Nicolas; Puchtel, Igor S.; Hofmann, Beda A.; Arndt, Nicholas T.

    2017-09-01

    Titanium isotopes are potential tracers of processes of evaporation/condensation in the solar nebula and magmatic differentiation in planetary bodies. To gain new insights into the processes that control Ti isotopic variations in planetary materials, 25 komatiites, 15 chondrites, 11 HED-clan meteorites, 5 angrites, 6 aubrites, a martian shergottite, and a KREEP-rich impact melt breccia have been analyzed for their mass-dependent Ti isotopic compositions, presented using the δ49Ti notation (deviation in permil of the 49Ti/47Ti ratio relative to the OL-Ti standard). No significant variation in δ49Ti is found among ordinary, enstatite, and carbonaceous chondrites, and the average chondritic δ49Ti value of +0.004 ± 0.010‰ is in excellent agreement with the published estimate for the bulk silicate Earth, the Moon, Mars, and the HED and angrite parent-bodies. The average δ49Ti value of komatiites of -0.001 ± 0.019‰ is also identical to that of the bulk silicate Earth and chondrites. OL-Ti has a Ti isotopic composition that is indistinguishable from chondrites and is therefore a suitable material for reporting δ49Ti values. Previously published isotope data on another highly refractory element, Ca, show measurable variations among chondrites. The decoupling between Ca and Ti isotope systematics most likely occurred during condensation in the solar nebula. Aubrites exhibit significant variations in δ49Ti, from -0.07 to +0.24‰. This is likely due to the uniquely reducing conditions under which the aubrite parent-body differentiated, allowing chalcophile Ti3+ and lithophile Ti4+ to co-exist. Consequently, the observed negative correlation between δ49Ti values and MgO concentrations among aubrites is interpreted to be the result of isotope fractionation driven by the different oxidation states of Ti in this environment, such that isotopically heavy Ti4+ was concentrated in the residual liquid during magmatic differentiation. Finally, KREEPy impact melt breccia

  13. The use of carbon stable isotope ratios in drugs characterization

    Energy Technology Data Exchange (ETDEWEB)

    Magdas, D. A., E-mail: gabriela.cristea@itim-cj.ro; Cristea, G., E-mail: gabriela.cristea@itim-cj.ro; Bot, A., E-mail: gabriela.cristea@itim-cj.ro; Mirel, V., E-mail: gabriela.cristea@itim-cj.ro [National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath Str., 400293 Cluj-Napoca (Romania)

    2013-11-13

    Isotopic Ratio Mass Spectrometry (IRMS) is an effective toll to be used for drug product authentication. The isotopic composition could be used to assist in the differentiation between batches of drugs and assist in the identification of counterfeit materials on the market. Only two factors affect the isotopic ratios in pharmaceutical components: the isotopic composition of the raw materials and the synthetic processes performed upon them. Counterfeiting of pharmaceutical drugs threatens consumer confidence in drug products companies' economical well-being. In this preliminary study, the analyzed samples consist in two types of commercially available analgesics, which were purchases from Romanian pharmacies. Differences in δ{sup 13}C between batches from −29.7 to −31.6% were observed, demonstrating that this method can be used to differentiate among individual drug batches and subsequently identify counterfeits on the market. On the other hand, carbon isotopic ratios differences among producers were recorded, the variations being between −31.3 to −34.9% for the same type of analgesic, but from different manufactures.

  14. A Test of Carbon and Oxygen Stable Isotope Ratio Process Models in Tree Rings.

    Science.gov (United States)

    Roden, J. S.; Farquhar, G. D.

    2008-12-01

    Stable isotopes ratios of carbon and oxygen in tree ring cellulose have been used to infer environmental change. Process-based models have been developed to clarify the potential of historic tree ring records for meaningful paleoclimatic reconstructions. However, isotopic variation can be influenced by multiple environmental factors making simplistic interpretations problematic. Recently, the dual isotope approach, where the variation in one stable isotope ratio (e.g. oxygen) is used to constrain the interpretation of variation in another (e.g. carbon), has been shown to have the potential to de-convolute isotopic analysis. However, this approach requires further testing to determine its applicability for paleo-reconstructions using tree-ring time series. We present a study where the information needed to parameterize mechanistic models for both carbon and oxygen stable isotope ratios were collected in controlled environment chambers for two species (Pinus radiata and Eucalyptus globulus). The seedlings were exposed to treatments designed to modify leaf temperature, transpiration rates, stomatal conductance and photosynthetic capacity. Both species were grown for over 100 days under two humidity regimes that differed by 20%. Stomatal conductance was significantly different between species and for seedlings under drought conditions but not between other treatments or humidity regimes. The treatments produced large differences in transpiration rate and photosynthesis. Treatments that effected photosynthetic rates but not stomatal conductance influenced carbon isotope discrimination more than those that influenced primarily conductance. The various treatments produced a range in oxygen isotope ratios of 7 ‰. Process models predicted greater oxygen isotope enrichment in tree ring cellulose than observed. The oxygen isotope ratios of bulk leaf water were reasonably well predicted by current steady-state models. However, the fractional difference between models that

  15. Dietary interpretations for extinct megafauna using coprolites, intestinal contents and stable isotopes: Complimentary or contradictory?

    Science.gov (United States)

    Rawlence, Nicolas J.; Wood, Jamie R.; Bocherens, Herve; Rogers, Karyne M.

    2016-06-01

    For many extinct species, direct evidence of diet (e.g. coprolites, gizzard/intestinal contents) is not available, and indirect dietary evidence (e.g. stable isotopes) must be relied upon. The Late Holocene fossil record of New Zealand provides a unique opportunity to contrast palaeodietary reconstructions for the extinct moa (Aves: Dinornithiformes) using stable isotopes and coprolite/gizzard contents. Palaeodietary reconstructions from isotopes are found to contradict those based on direct dietary evidence. We discuss reasons for this and advocate, where possible, for the use of multiple lines of evidence in reconstructing the diets of extinct species.

  16. Stable isotope composition and volume of Early Archaean oceans

    DEFF Research Database (Denmark)

    Pope, Emily Catherine; Rosing, Minik Thorleif; Bird, Dennis K.

    Oxygen and hydrogen isotope compositions of seawater are controlled by volatile fluxes between mantle, lithospheric (oceanic and continental crust) and atmospheric reservoirs. Throughout geologic time oxygen was likely conserved within these Earth system reservoirs, but hydrogen was not, as it can...... escape to space [1]. Hydrogen isotope ratios of serpentinites from the ~3.8Ga Isua Supracrustal Belt in West Greenland are between -53 and -99‰; the highest values are in antigorite ± lizardite serpentinites from a low-strain lithologic domain where hydrothermal reaction of Archaean seawater with oceanic...... of continents present at that time), and the mass of Early Archaean oceans to ~109 to 126% of present day oceans. Oxygen isotope analyses from these Isua serpentinites (δ18O = +0.1 to 5.6‰ relative to VSMOW) indicate that early Archaean δ18OSEAWATER similar to modern oceans. Our observations suggest...

  17. Photosynthetic fractionation of the stable isotopes of oxygen and carbon

    Energy Technology Data Exchange (ETDEWEB)

    Guy, R.D. (Carnegie Institution of Washington, Stanford, CA (United States)); Fogel, M.L.; Berry, J.A. (Carnegie Inst. of Washington, Washington, DC (United States))

    1993-01-01

    Isotope discrimination during photosynthetic exchange of O[sub 2] and CO[sub 2] was measured using enzyme, thylakoid, and whole cell preparations. Evolved oxygen from isolated spinach thylakoids was isotopically identical (within analytical error) to its source water. Similar results were obtained with Anacystis nidulans Richter and Phaeodactylum tricornutum Bohlin cultures purged with helium. For consumptive reactions, discrimination ([triangle], where 1 + [triangle]/1000 equals the isotope effect, k[sup 16]/k[sup 18] or k[sup 12]/k[sup 13]) was determined by analysis of residual substrate (O[sub 2] or CO[sub 2]). The [triangle] for the Mehler reaction, mediated by ferredoxin or methylviologen, was 15.3[per thousand]. Oxygen isotope discrimination during oxygenation of ribulose-1,5-bisphosphate (RuBP) catalyzed by RuBP carboxylase/oxygenase (Rubisco) was 21.3[per thousand] and independent of enzyme source, unlike carbon isotope dicrimination: 30.3[per thousand] for spinach enzyme and 19.6 to 23[per thousand] for Rhodospirillum rubrum and A. nidulans enzymes, depending on reaction conditions. The [triangle] for O[sub 2] consumption catalyzed by glycolate oxidase was 22.7[per thousand]. Consistent with this, when Asparagus sprengeri Regel mesopyll cells approached the compensation point within a sealed vessel, the [delta][sup 18]O of dissolved O[sub 2] came to a steady-state value of about 21.5[per thousand] relative to the source water. The results provide improved estimates of discrimination factors in several reactions prominent in the global oxygen cycle and indicate that photorespiration plays a significant part in determining the isotopic composition of atmospheric oxygen. 47 refs., 8 figs., 2 tabs.

  18. Stable isotope composition and volume of Early Archaean oceans

    DEFF Research Database (Denmark)

    Pope, Emily Catherine; Rosing, Minik Thorleif; Bird, Dennis K.

    Oxygen and hydrogen isotope compositions of seawater are controlled by volatile fluxes between mantle, lithospheric (oceanic and continental crust) and atmospheric reservoirs. Throughout geologic time oxygen was likely conserved within these Earth system reservoirs, but hydrogen was not, as it can...... escape to space [1]. Hydrogen isotope ratios of serpentinites from the ~3.8Ga Isua Supracrustal Belt in West Greenland are between -53 and -99‰; the highest values are in antigorite ± lizardite serpentinites from a low-strain lithologic domain where hydrothermal reaction of Archaean seawater with oceanic...

  19. Progresses in Applications of Stable Isotope Technology to Determining Geographical Origins of Traditional Chinese Medicines

    Directory of Open Access Journals (Sweden)

    WANG Li-ming

    2015-11-01

    Full Text Available Identification of proper geographical origin of traditional Chinese medicine is critical for guaranteeing the quality and efficacy of the medicine, safeguarding the market order, and reducing the medical negligence rate due to fake products. Stable isotope technology, as one of the most efficient methods to determine agricultural products authenticity and traceability, have great advantages and theoretical basis for determining geo-origin of traditional Chinese medicine, and it has been applied to many expensive herbs. In this review, the basic principles of isotopic traceability were firstly introduced. We then elaborate in details the frequently-used isotopic indicators in the geo origin studies of traditional Chinese medicine. We also provide a summary of current research progresses and point out some directions for future research. The purpose of this paper is to promote the applications of stable isotopes to traditional Chinese medicine traceability studies and the establishment of the isotopic database and the improvement of the medicine traceability.

  20. Application of stable isotope ratio analysis for biodegradation monitoring in groundwater

    Science.gov (United States)

    Hatzinger, Paul B.; Böhlke, John Karl; Sturchio, Neil C.

    2013-01-01

    Stable isotope ratio analysis is increasingly being applied as a tool to detect, understand, and quantify biodegradation of organic and inorganic contaminants in groundwater. An important feature of this approach is that it allows degradative losses of contaminants to be distinguished from those caused by non-destructive processes such as dilution, dispersion, and sorption. Recent advances in analytical techniques, and new approaches for interpreting stable isotope data, have expanded the utility of this method while also exposing complications and ambiguities that must be considered in data interpretations. Isotopic analyses of multiple elements in a compound, and multiple compounds in the environment, are being used to distinguish biodegradative pathways by their characteristic isotope effects. Numerical models of contaminant transport, degradation pathways, and isotopic composition are improving quantitative estimates of in situ contaminant degradation rates under realistic environmental conditions.

  1. Plasma separation process facility for large-scale stable isotope production

    Energy Technology Data Exchange (ETDEWEB)

    Bigelow, T.S.; Collins, E.D.; Tracy, J.G. [Oak Ridge National Lab., TN (United States)

    1997-12-01

    A facility for large-scale separation of stable isotopes using the plasma separation process (PSP) is under development at the Oak Ridge National Laboratory. The PSP is capable of separating isotopes at a large throughput rate with medium purity product and at relatively low cost. The PSP has a number of convenient features that make it an attractive technology for general isotope separation purposes. Several isotopes for medical and industrial applications, including {sup 102}Pd, {sup 98}Mo, {sup 203}Tl, {sup 184}W, and others, are expected to be processed in this facility. The large throughput and low processing cost of the PSP will likely lead to new applications for stable isotopes. A description of this facility and its typical throughput capability is presented here.

  2. Stable isotope ecology of a hyper-diverse community of scincid lizards from arid Australia.

    Science.gov (United States)

    Grundler, Maggie R; Pianka, Eric R; Pelegrin, Nicolás; Cowan, Mark A; Rabosky, Daniel L

    2017-01-01

    We assessed the utility of stable isotope analysis as a tool for understanding community ecological structure in a species-rich clade of scincid lizards from one of the world's most diverse lizard communities. Using a phylogenetic comparative framework, we tested whether δ15N and δ13C isotopic composition from individual lizards was correlated with species-specific estimates of diet and habitat use. We find that species are highly divergent in isotopic composition with significant correlations to habitat use, but this relationship shows no phylogenetic signal. Isotopic composition corresponds to empirical observations of diet for some species but much variation remains unexplained. We demonstrate the importance of using a multianalytical approach to questions of long-term dietary preference, and suggest that the use of stable isotopes in combination with stomach content analysis and empirical data on habitat use can potentially reveal patterns in ecological traits at finer scales with important implications for community structuring.

  3. Progress and challenges in using stable isotopes to trace plant carbon and water relations across scales

    Directory of Open Access Journals (Sweden)

    C. Werner

    2012-08-01

    Full Text Available Stable isotope analysis is a powerful tool for assessing plant carbon and water relations and their impact on biogeochemical processes at different scales. Our process-based understanding of stable isotope signals, as well as technological developments, has progressed significantly, opening new frontiers in ecological and interdisciplinary research. This has promoted the broad utilisation of carbon, oxygen and hydrogen isotope applications to gain insight into plant carbon and water cycling and their interaction with the atmosphere and pedosphere. Here, we highlight specific areas of recent progress and new research challenges in plant carbon and water relations, using selected examples covering scales from the leaf to the regional scale. Further, we discuss strengths and limitations of recent technological developments and approaches and highlight new opportunities arising from unprecedented temporal and spatial resolution of stable isotope measurements.

  4. Chromium stable isotope fractionation in modern biogeochemical cycling

    DEFF Research Database (Denmark)

    Paulukat, Cora Stefanie

    Chromium (Cr) is, due to its redox-sensitive properties, a powerful tracer for redox processes in environmental studies. Changes in its preferred oxidation state (III and VI) are accompanied by Crisotope fractionation. The Cr-isotope system is a promising tool to reconstruct the evolution of free...

  5. The use of stable isotopes to trace oil sands constituents

    Energy Technology Data Exchange (ETDEWEB)

    Farwell, A.J.; Nero, V.; Dixon, D.G. [Waterloo Univ., ON (Canada). Dept. of Biology

    2002-07-01

    A study was conducted to determine the biological effects of oil sands mining operations on aquatic ecosystems. The study focused on the Athabasca oil sand deposit, the largest of 4 deposits in northern Alberta. In particular, the study examined the cycling of oil sand constituents in Benthic invertebrates collected from test pits at Syncrude Canada Ltd.. The invertebrates were similar in size, but different in the quantity of process-affected water or mature fine tailings containing residual bitumen. Dragonflies and damselflies in particular, showed trends of depletion for the carbon 13 isotope and enrichment in nitrogen 15 isotope in pits where levels of process affected water was high. The depletion of carbon 13 isotope suggests that oil sand constituents assimilate into the benthic food chain. The greatest carbon 13 depletion, which was approximately 27 per cent, was found to be in test pits with high turbidity. This implies that oil sands constituents degrade microbially instead of by photosynthetic production. All benthic invertebrate group demonstrated an incremental enrichment in nitrogen 15 isotope from the control pit to the pit with greatest levels of mature fine tailings.

  6. Equations for lipid normalization of carbon stable isotope ratios in aquatic bird eggs.

    Directory of Open Access Journals (Sweden)

    Kyle H Elliott

    Full Text Available Stable isotope ratios are biogeochemical tracers that can be used to determine the source of nutrients and contaminants in avian eggs. However, the interpretation of stable carbon ratios in lipid-rich eggs is complicated because (13C is depleted in lipids. Variation in (13C abundance can therefore be obscured by variation in percent lipids. Past attempts to establish an algebraic equation to correct carbon isotope ratios for lipid content in eggs have been unsuccessful, possibly because they relied partly on data from coastal or migratory species that may obtain egg lipids from different habitats than egg protein. We measured carbon, nitrogen and sulphur stable isotope ratios in 175 eggs from eight species of aquatic birds. Carbon, nitrogen and sulphur isotopes were enriched in lipid-extracted egg samples compared with non extracted egg samples. A logarithmic equation using the C∶N ratio and carbon isotope ratio from the non extracted egg tissue calculated 90% of the lipid-extracted carbon isotope ratios within ±0.5‰. Calculating separate equations for eggs laid by species in different habitats (pelagic, offshore and terrestrial-influenced improved the fit. A logarithmic equation, rather than a linear equation as often used for muscle, was necessary to accurately correct for lipid content because the relatively high lipid content of eggs compared with muscle meant that a linear relationship did not accurately approximate the relationship between percent lipids and the C∶N ratio. Because lipid extraction alters sulphur and nitrogen isotope ratios (and cannot be corrected algebraically, we suggest that isotopic measurement on bulk tissue followed by algebraic lipid normalization of carbon stable isotope ratio is often a good solution for homogenated eggs, at least when it is not possible to complete separate chemical analyses for each isotope.

  7. Isotopic tracing of clear water sources in an urban sewer: A combined water and dissolved sulfate stable isotope approach.

    Science.gov (United States)

    Houhou, J; Lartiges, B S; France-Lanord, C; Guilmette, C; Poix, S; Mustin, C

    2010-01-01

    This paper investigates the potential of stable isotopes of both water (deltaD and deltaOH(2)O18) and dissolved sulfate (delta(34)S and deltaOSO(4)18) for determining the origin and the amount of clear waters entering an urban sewer. The dynamics of various hydrological processes that commonly occur within the sewer system such as groundwater infiltration, rainwater percolation, or stormwater release from retention basins, can be readily described using water isotope ratios. In particular, stable water isotopes indicate that the relative volumes of infiltrated groundwater and sewage remain approximately constant and independent of wastewater flow rate during the day, thus demonstrating that the usual quantification of parasitic discharge from minimal nocturnal flow measurements can lead to completely erroneous results. The isotopic signature of dissolved sulfate can also provide valuable information about the nature of water inputs to the sewage flow, but could not be used in our case to quantify the infiltrating water. Indeed, even though the microbial activity had a limited effect on the isotopic composition of dissolved sulfate at the sampling sites investigated, the dissolved sulfate concentration in sewage was regulated by the formation of barite and calcium-phosphate mineral species. Sulfate originating from urine was also detected as a source using the oxygen isotopic composition of sulfate, which suggests that deltaOSO(4)18 might find use as a urine tracer.

  8. Stable isotopes of authigenic minerals in variably-saturated fractured tuff

    Energy Technology Data Exchange (ETDEWEB)

    Weber, D.S.; Evans, D.D.

    1988-11-01

    Identifying stable isotope variation and mineralogical changes in fractured rock may help establish the history of climatic and geomorphological processes that might affect the isolation properties of a waste repository site. This study examines the use of the stable isotope ratios of oxygen ({sup 18}O/{sup 16}O) and carbon ({sup 13}C/{sup 12}C) in authigenic minerals as hydrogeochemical tools tracing low-temperature rock-water interaction in variably-saturated fractured stuff. Isotopic compositions of fracture-filling and rock matrix minerals in the Apache Leap tuff, near Superior, Arizona were concordant with geothermal temperatures and in equilibrium with water isotopically similar to present-day meteoric water and groundwater. Oxygen and carbon isotope ratios of fracture-filling, in unsaturated fractured tuff, displayed an isotopic gradient believed to result from near-surface isotopic enrichment due to evaporation rather than the effects of rock-water interaction. Oxygen isotope ratios of rock matrix opal samples exhibited an isotopic gradient believed to result from, leaching and reprecipitation of silica at depth. Methods and results can be used to further define primary flowpaths and the movement of water in variably-saturated fractured rock. 71 refs., 23 figs., 3 tabs.

  9. Pooled versus separate measurements of tree-ring stable isotopes.

    Science.gov (United States)

    Dorado Liñán, Isabel; Gutiérrez, Emilia; Helle, Gerhard; Heinrich, Ingo; Andreu-Hayles, Laia; Planells, Octavi; Leuenberger, Markus; Bürger, Carmen; Schleser, Gerhard

    2011-05-01

    δ(13)C and δ(18)O of tree rings contain time integrated information about the environmental conditions weighted by seasonal growth dynamics and are well established as sources of palaeoclimatic and ecophysiological data. Annually resolved isotope chronologies are frequently produced by pooling dated growth rings from several trees prior to the isotopic analyses. This procedure has the advantage of saving time and resources, but precludes from defining the isotopic error or statistical uncertainty related to the inter-tree variability. Up to now only a few studies have compared isotope series from pooled tree rings with isotopic measurements from individual trees. We tested whether or not the δ(13)C and the δ(18)O chronologies derived from pooled and from individual tree rings display significant differences at two locations from the Iberian Peninsula to assess advantages and constraints of both methodologies. The comparisons along the period 1900-2003 reveal a good agreement between pooled chronologies and the two mean master series which were created by averaging raw individual values (Mean) or by generating a mass calibrated mean (MassC). In most of the cases, pooled chronologies show high synchronicity with averaged individual samples at interannual scale but some differences also show up especially when comparing δ(18)O decadal to multi-decadal variations. Moreover, differences in the first order autocorrelation among individuals may be obscured by pooling strategies. The lack of replication of pooled chronologies prevents detection of a bias due to a higher mass contribution of one sample but uncertainties associated with the analytical process itself, as sample inhomogeneity, seems to account for the observed differences.

  10. Isotopic Analysis of Fingernails as a USGS Open House Demonstration of the Use of Stable Isotopes in Foodweb Studies

    Science.gov (United States)

    Silva, S. R.; Kendall, C.; Young, M. B.; Choy, D.

    2011-12-01

    The USGS Isotope Tracers Project uses stable isotopes and tritium to add a unique dimension of chemical information to a wide range of environmental investigations. The use and application of isotopes is usually an unfamiliar and even esoteric topic to the general public. Therefore during three USGS open house events, as a public outreach effort, we demonstrated the use of stable isotopes by analyzing nitrogen and carbon isotopes from very small fragments of fingernail from willing participants. We titled the exhibit "You Are What You Eat". The results from all participants were plotted on a graph indicating the general influence of different food groups on the composition of body tissues as represented by fingernails. All participants were assigned a number and no personal-identification information was collected. A subset of participants provided us with an estimate of the number of days a week various foods were eaten and if they were vegetarians, vegans or non-vegetarians. Volunteers from our research group were on hand to explain and discuss fundamental concepts such as how foods attain their isotopic composition, the difference between C3 and C4 plants, the effects of assimilation, trophic enrichment, and the various uses of stable isotopes in environmental studies. The results of the fingernail analyses showed the variation of the range of isotopic compositions among about 400 people at each event, the distinct influence of C4 plants (mainly corn and cane sugar) on our carbon isotopic composition, and the isotopic differences between vegetarians and non vegetarians among other details (http://wwwrcamnl.wr.usgs.gov/isoig/projects/fingernails/). A poll of visitors attending the open house event in 2006 indicated that "You Are What You Eat" was among the most popular exhibits. Following the first two open house events we were contacted by a group of researchers from Brazil who had completed a very similar study. Our collaboration resulted in a publication in

  11. Using Stable Isotopes to Trace Microbial Hydrogen Production Pathways

    Science.gov (United States)

    Moran, J.; Hill, E.; Bartholomew, R.; Yang, H.; Shi, L.; Ostrom, N. E.; Gandhi, H.; Hegg, E.; Kreuzer, H.

    2010-12-01

    Biological H2 production by hydrogenase enzymes (H2ases) plays an important role in anaerobic microbial metabolism and community structure. Despite considerable progress in elucidating H2 metabolism, the regulation of and flux through key H2 production pathways remain largely undefined. Our goal is to improve understanding of biological H2 production by using H isotope ratios to dissect proton fluxes through different H2ase enzymes and from different substrates. We hypothesized that the isotope ratio of H2 produced by various hydrogenases (H2ase) would differ, and that the H isotope ratios would allow us to define the contribution of different enzymes when more than one is present in vivo. We chose Shewanella oneidensis (S.o.) MR-1, a facultative anaerobe capable of transferring electrons to a variety of terminal acceptors, including protons, as a model system for in vivo studies. S. o. encodes one [FeFe]- and one [NiFe]-H2ase. We purified three [FeFe]-H2ases (S.o., Clostridium pasteurianum, and Chlamydomonas reinhardtii) and two [NiFe]-H2ases (S. o. and Desulfovibrio fructosovorans) to test the isotope fractionation associated with activity by each enzyme in vitro. For in vivo analysis we used wild-type S.o. as well as electron transfer-deficient and H2ase-deficient strains. We employed batch cultures using lactate as an electron donor and O2 as an initial electron acceptor (with H2 production after O2 consumption). The five H2ases we tested all had a unique isotope fractionation. Measurements of H2 produced in vivo showed distinct periods of H2 production having isotope signatures consistent with in vitro results. Isotope data as well as studies of H2 production by mutants in the genes encoding either the [NiFe]-H2ase or the [FeFe]-H2ase, respectively, show that the [NiFe]- and [FeFe]- H2ases became active at different times. The [NiFe]-H2ase both produces and consumes H2 before the [FeFe]-H2ase becomes active. RNA analysis is consistent with up regulation of

  12. Environmental geochemistry of calcium isotopes: Applications of a new stable isotope approach

    Institute of Scientific and Technical Information of China (English)

    LIU Zhanmin; LIU Congqiang; HAN Guilin; WANG Zhongliang; XUE Zichen; SONG Zhaoliang; YANG Cheng

    2006-01-01

    This paper summarizes isotope fractionation mechanism, analytical method and applications in environmental geochemistry of calcium isotopes. Calcium isotopic composition can be used to constrain material sources and study geological and environmental processes as the isotopic composition of calcium (δ 44Ca) and fractionation processes depend on geochemical circumstances in nature. Recently, thanks to current advances in analytical technology of calcium isotopes, calcium isotopes are broadly used in biological and geochemical studies, such as the mechanism of plants imbibing nutrients through their roots, calcium transport in the environmental ecosystem, calcium cycle in oceans and paleo-oceans and paleo-climate. The elementary data show that δ44Ca values vary from -2.88‰ to 0.92‰ in natural samples.

  13. Stable isotope studies of nephrite deposits from Fengtien, Taiwan

    Energy Technology Data Exchange (ETDEWEB)

    Yui, Tzenfu; Yeh, Hsuehwen (Univ. of Hawaii, Honolulu (USA)); Lee, Chihming Wang (National Taiwan Univ. Taipei (Taiwan))

    1988-03-01

    Metasomatic nephrite deposits (i.e., rodingites) occur between serpentinites and muscovite-quartz schist in the Fengtien area, eastern Taiwan. The {delta}{sup 18}O values of nephrite range from +4.5 to +5.3{per thousand} and the {delta}D values, from {minus}33 to {minus}68{per thousand}. The narrow range in the {delta}{sup 18}O values and wide variation in the {delta}D values indicate low water/rock ratios and multiple origins of the ambient H{sub 2}O during nephrite formation. The ultimate sources of the ambient H{sub 2}O are largely sea water for the early nephrite and meteoric water for the late nephrite. The temperature of nephrite formation is 320-420{degree}C: the estimated O-isotopic fractionation between nephrite (tremolite) and serpentine (antigorite) is 1.0 {plus minus} 0.2{per thousand} in this temperature range. Diopsidefels and epidotite are two other kinds of rodingite in the deposits. The {delta}{sup 18}O values of diopside from diopsidefels range from +3.7 to +4.5{per thousand}, the {delta}D values of clinozoisite from epidotite range from {minus}31 to {minus}34{per thousand} and the {delta}{sup 18}O value of one clinozoisite analyzed is +3.9{per thousand}. These data suggest that most diopsidefels formed in isotopic equilibrium with nephrite and that clinozoisite was not in isotopic equilibrium with either nephrite of diopsidefels. O-isotope compositions of both nephrite and diopsidefels cannot give conclusive evidence of their parental rocks. Circumstantial evidence, however, implies that the nephrite is derived from serpentinite whereas diopsidefels formed from unaltered ultramafic rocks.

  14. Stable isotope studies of nephrite deposits from Fengtien, Taiwan

    Science.gov (United States)

    Yui, Tzen-Fu; Yeh, Hsueh-Wen; Lee, Chihming Wang

    1988-03-01

    Metasomatic nephrite deposits ( i.e., rodingites) occur between serpentinites and muscovite-quartz schist in the Fengtien area, eastern Taiwan. The δ 18O values of nephrite range from +4.5 to +5.3%. and the δD values, from -33 to -68%. The narrow range in the δ 18O values and wide variation in the δD values indicate low water/rock ratios and multiple origins of the ambient H 2O during nephrite formation. The ultimate sources of the ambient H 2O are largely sea water for the early nephrite and meteoric water for the late nephrite. The temperature of nephrite formation is 320-420°C: the estimated O-isotopic fractionation between nephrite (tremolite) and serpentine (antigorite) is 1.0 ± 0.2%. in this temperature range. Diopsidefels and epidotite are two other kinds of rodingite in the deposits. The δ 18O values of diopside from diopsidefels range from +3.7 to +4.5%., theδD values of clinozoisite from epidotite range from -31 to -34%. and the δ 18O value of one clinozoisite analyzed is +3.9%. These data suggest that most diopsidefels formed in isotopic equilibrium with nephrite and that clinozoisite was not in isotopic equilibrium with either nephrite or diopsidefels. O-isotope compositions of both nephrite and diopsidefels cannot give conclusive evidence of their parental rocks. Circumstantial evidence, however, implies that the nephrite is derived from serpentinite whereas diopsidefels formed from unaltered ultramafic rocks.

  15. Stable isotope enrichment techniques and ORNL separation status

    Energy Technology Data Exchange (ETDEWEB)

    Tracy, J.G.; Bell, W.A.; Veach, A.M.; Caudill, H.H.; Milton, H.T.

    1987-05-01

    The isotope separation program is described, emphasizing present state-of-the-art techniques utilized to achieve specific isotopic requirements. An interesting problem addressed here is the calutron enrichment of rare-earth isotopes where small quantities of feed (<5 g) are available, and the unresolved feed is to be recovered and recycled. Conventional ion-source units using graphite and stainless steel deteriorate in the halogenating atmosphere or are permeable to rare-earth compounds, reducing the process efficiency. An ion source has been developed using boron nitride for containing the halogenating agent and rare-earth compounds. Tests have been successfully conducted using Lu/sub 2/O/sub 3/ and the in situ chlorinating technique with CCl/sub 4/. Collectively, 166 mg of /sup 176/Lu were recovered from two runs using 2.95 and 1.10 g of 44.5% /sup 176/Lu. Process efficiency of 10.5% was achieved, and 1.2 g of the unresolved feed were recovered. Material compatibility of the boron nitride, carbon tetrachloride, and lutetium compounds has been established.

  16. Stable isotope enrichment techniques and ORNL separation status

    Science.gov (United States)

    Tracy, J. G.; Bell, W. A.; Veach, A. M.; Caudill, H. H.; Milton, H. T.

    1987-05-01

    The isotope separation program is described, emphasizing present state-of-the-art techniques utilized to achieve specific isotopic requirements. An interesting problem addressed here is the calutron enrichment of rare-earth isotopes where small quantities of feed (< 5 g) are available, and the unresolved feed is to be recovered and recycled. Conventional ion-source units using graphite and stainless steel deteriorate in the halogenating atmosphere or are permeable to rare-earth compounds, reducing the process efficiency. An ion source has been developed using boron nitride for containing the halogenating agent and rare-earth compounds. Tests have been successfully conducted using Lu 2O 3 and the in situ chlorinating technique with CCl 4. Collectively, 166 mg of 176Lu were recovered from two runs using 2.95 and 1.10 g of 44.5% 176Lu. Process efficiency of 10.5% was achieved, and 1.2 g of the unresolved feed were recovered. Material compatibility of the boron nitride, carbon tetrachloride, and lutetium compounds has been established.

  17. Pooled versus separate measurements of tree-ring stable isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Dorado Linan, Isabel, E-mail: isabel@gfz-potsdam.de [Universitat de Barcelona, Departament d' Ecologia, Diagonal 645, 08028, Barcelona (Spain); German Centre for Geosciences, Climate Dynamics and Landscape Evolution, Dendro Laboratory, Telegrafenberg, 14473, Potsdam (Germany); Gutierrez, Emilia, E-mail: emgutierrez@ub.edu [Universitat de Barcelona, Departament d' Ecologia, Diagonal 645, 08028, Barcelona (Spain); Helle, Gerhard, E-mail: ghelle@gfz-potsdam.de [German Centre for Geosciences, Climate Dynamics and Landscape Evolution, Dendro Laboratory, Telegrafenberg, 14473, Potsdam (Germany); Heinrich, Ingo, E-mail: heinrich@gfz-potsdam.de [German Centre for Geosciences, Climate Dynamics and Landscape Evolution, Dendro Laboratory, Telegrafenberg, 14473, Potsdam (Germany); Andreu-Hayles, Laia, E-mail: laiandreu@ub.edu [Universitat de Barcelona, Departament d' Ecologia, Diagonal 645, 08028, Barcelona (Spain); Tree-Ring Laboratory, Lamont-Doherty Earth Observatory of Columbia University, Palisades NY (United States); Planells, Octavi, E-mail: leocarpus@hotmail.com [Universitat de Barcelona, Departament d' Ecologia, Diagonal 645, 08028, Barcelona (Spain); Leuenberger, Markus, E-mail: leuenberger@climate.unibe.ch [Climate and Environmental Physics, Physics Institute, University of Bern, Sidlerstrasse 5, 3012 Bern (Switzerland); Oeschger Centre of Climate Change Research, University of Bern, Zaehringerstrasse 25, 3012 Bern (Switzerland); Buerger, Carmen, E-mail: buerger@gfz-potsdam.de [German Centre for Geosciences, Climate Dynamics and Landscape Evolution, Dendro Laboratory, Telegrafenberg, 14473, Potsdam (Germany); Schleser, Gerhard, E-mail: schleser@gfz-potsdam.de [German Centre for Geosciences, Climate Dynamics and Landscape Evolution, Dendro Laboratory, Telegrafenberg, 14473, Potsdam (Germany)

    2011-05-01

    {delta}{sup 13}C and {delta}{sup 18}O of tree rings contain time integrated information about the environmental conditions weighted by seasonal growth dynamics and are well established as sources of palaeoclimatic and ecophysiological data. Annually resolved isotope chronologies are frequently produced by pooling dated growth rings from several trees prior to the isotopic analyses. This procedure has the advantage of saving time and resources, but precludes from defining the isotopic error or statistical uncertainty related to the inter-tree variability. Up to now only a few studies have compared isotope series from pooled tree rings with isotopic measurements from individual trees. We tested whether or not the {delta}{sup 13}C and the {delta}{sup 18}O chronologies derived from pooled and from individual tree rings display significant differences at two locations from the Iberian Peninsula to assess advantages and constraints of both methodologies. The comparisons along the period 1900-2003 reveal a good agreement between pooled chronologies and the two mean master series which were created by averaging raw individual values (Mean) or by generating a mass calibrated mean (MassC). In most of the cases, pooled chronologies show high synchronicity with averaged individual samples at interannual scale but some differences also show up especially when comparing {delta}{sup 18}O decadal to multi-decadal variations. Moreover, differences in the first order autocorrelation among individuals may be obscured by pooling strategies. The lack of replication of pooled chronologies prevents detection of a bias due to a higher mass contribution of one sample but uncertainties associated with the analytical process itself, as sample inhomogeneity, seems to account for the observed differences. - Research Highlights: {yields} Pooled {delta}{sup 13}C and {delta}{sup 18}O chronologies are expected to be similar to the mean. {yields} Empirical pooled chronologies {delta}{sup 13}C and

  18. Stable Isotopes Indicate Within-Canopy Processes During Interception of Rainfall

    Science.gov (United States)

    Allen, S. T.; Keim, R.; Barnard, H. R.; Brooks, J. R.; McDonnell, J.

    2015-12-01

    Stable isotopes of water have been used to gain process-level understand of mixing, storage, and transport in all components of the hydrological cycle. Canopy interception processes remain some of the least understood because of the relatively small storage pool, rapid turnover, and variability at short intervals relative to, for example, soils. Stable isotopes provide a look into the 'black box' of canopy processes that control interception storage and throughfall generation. Several recent studies have compared throughfall isotopic composition to open rainfall; canopy effects vary in direction and magnitude but are ubiquitous. We present findings from three studies using isotopes of throughfall. In all cases, common patterns and persistence of patterns in variability of throughfall amount (e.g., correlation with canopy characteristics, correlations with precipitation characteristics, and geostatistical relationships) were infrequently apparent for isotopic composition. Data consistently support the so called 'selection' effect, that throughfall composition is a product of spatially and temporally varying transmission of rainfall that has temporally varying isotopic composition. There is little evidence of isotopic fractionation by wet-canopy evaporation. Additionally, isotopic composition of storm-total throughfall is generally less variable than is amount or solute content, suggesting two possibilities: (1) high spatial homogeneity in the selection effect, or (2) rapid exchange and equilibration of droplets with vapor in the canopy airspace, lending support to the hypothesized role of splash droplet evaporation. These results suggest a need to re-examine conceptual models of the progression from interception to evaporation and throughfall generation.

  19. Monitoring of the aerobe biodegradation of chlorinated organic solvents by stable isotope analysis

    Science.gov (United States)

    Horváth, Anikó; Futó, István; Palcsu, László

    2014-05-01

    Our chemical-biological basic research aims to eliminate chlorinated environmental contaminants from aquifers around industrial areas in the frame of research program supported by the European Social Fund (TÁMOP-4.2.2.A-11/1/KONV-2012-0043). The most careful and simplest way includes the in situ biodegradation with the help of cultured and compound specific strains. Numerous members of Pseudomonas bacteria are famous about function of bioremediation. They can metabolism the environmental hazardous chemicals like gas oils, dyes, and organic solvents. Our research based on the Pseudomonas putida F1 strain, because its ability to degrade halogenated hydrocarbons such as trichloroethylene. Several methods were investigated to estimate the rate of biodegradation, such as the measurement of the concentration of the pollutant along the contamination pathway, the microcosm's studies or the compound specific stable isotope analysis. In this area in the Transcarpathian basin we are pioneers in the stable isotope monitoring of biodegradation. The main goal is to find stable isotope fractionation factors by stable isotope analysis, which can help us to estimate the rate and effectiveness of the biodegradation. The subsequent research period includes the investigation of the method, testing its feasibility and adaptation in the environment. Last but not least, the research gives an opportunity to identify the producer of the contaminant based on the stable isotope composition of the contaminant.

  20. Stable isotope evolution and paleolimnology of ancient Lake Creede

    Science.gov (United States)

    Rye, Robert O.; Bethke, Philip M.; Finkelstein, David B.

    2000-01-01

    The lacustrine carbonate and travertine (tufa) deposits of ancient Lake Creede preserve a remarkable record of the isotopic evolution of the lake. That record indicates that the δ18O of the lake water, and by analogy its salinity, evolved through evaporation. Limited ans less reliable data on hydrous minerals and fluid inclusions in early diagenetic carbonates indicate that the δD of the lake waters also evolved through evaporation. The isotope data place restrictions on models of the physical limnology of the lake and its evolution. The closed-basin Lake Creede formed shortly after collapse of the 26.9 Ma Creede caldera. Throughout most of its history it occupied the northern three quarters of the moat between the resurgent dome and wall of the caldera. The Creede Formation was deposited in the basin, dominantly as lacustrine sediments. Travertine mounds interfinger with Creede Formation sediments along the inner and outer margins of the lake basin. An estimated one-half of the original thickness of the Creede Formation has been lost mainly to erosion although scattered remnants of the upper portion remain on the caldera walls. Two diamond core holes (CCM-1 and CCM-2) sampled the uneroded portion of the Creede Formation as part of the U.S. Continental Drilling Program. Volcaniclastic material, including tuff units deposited directly into the lake and ash washed in from the watershed, compose the main lithologies of the Creede Formation. These volcaniclastic strata were produced by episodic ring-fracture volcanism. Lacustrine carbonates make up about 15% of the section sampled by drill core. They occur as 1 mm to 2 cm low-Mg calcite laminar alternating with siliciclastic laminar in scattered intervals throughout the preserved section. The carbonate laminar are accumulations of 5-20 μm crystallites (microparites) and brine shrimmp fecal pellets (peloids) composed mainly of microparasite particles. Low-Mg calcite also occurs as an early diagenetic replacement of

  1. Evaluation of stable tungsten isotopes in the resolved resonance region

    Directory of Open Access Journals (Sweden)

    Schillebeeckx P.

    2013-03-01

    Full Text Available In the last decade benchmark experiments and simulations, together with newly obtained neutron cross section data, have pointed out deficiencies in evaluated data files of W isotopes. The role of W as a fundamental structural material in different nuclear applications fully justifies a new evaluation of 182, 183, 184, 186W neutron resonance parameters. In this regard transmission and capture cross section measurements on natural and enriched tungsten samples were performed at the GELINA facility of the EC-JRC-IRMM. A resonance parameter file used as input in the resonance shape analysis was prepared based on the available literature and adjusted in first instance to transmission data.

  2. Recent research activities and future subjects on stable- and radio-isotopes of chlorine in environment

    Energy Technology Data Exchange (ETDEWEB)

    Kushita, Kouhei [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment

    2001-12-01

    This report reviews the recent studies on the stable- and radio-isotopes of chlorine from a viewpoint of environmental science, partly including historic references on this element. First, general properties, occurrence, and utilization of chlorine are described. Secondly, current status and research works on chlorine-compounds, which attract special attention in recent years as environmentally hazardous materials, are reported. Thirdly, research works on stable chlorine isotopes, {sup 35}Cl and {sup 37}Cl, are described with a focus laid on the newly-developed techniques; isotopic ratio mass spectrometry (IRMS) and thermal ionization mass spectrometry (TIMS). Fourthly, recent research works on chlorine radioisotopes, {sup 36}Cl etc., are described, focusing on the development of accelerator mass spectrometry (AMS) and its application to geochemistry and others. Finally, taking account of the above-mentioned recent works on Cl isotopes, possible future research subjects are discussed. (author)

  3. A new method of snowmelt sampling for water stable isotopes

    Science.gov (United States)

    Penna, D.; Ahmad, M.; Birks, S. J.; Bouchaou, L.; Brencic, M.; Butt, S.; Holko, L.; Jeelani, G.; Martinez, D. E.; Melikadze, G.; Shanley, J.B.; Sokratov, S. A.; Stadnyk, T.; Sugimoto, A.; Vreca, P.

    2014-01-01

    We modified a passive capillary sampler (PCS) to collect snowmelt water for isotopic analysis. Past applications of PCSs have been to sample soil water, but the novel aspect of this study was the placement of the PCSs at the ground-snowpack interface to collect snowmelt. We deployed arrays of PCSs at 11 sites in ten partner countries on five continents representing a range of climate and snow cover worldwide. The PCS reliably collected snowmelt at all sites and caused negligible evaporative fractionation effects in the samples. PCS is low-cost, easy to install, and collects a representative integrated snowmelt sample throughout the melt season or at the melt event scale. Unlike snow cores, the PCS collects the water that would actually infiltrate the soil; thus, its isotopic composition is appropriate to use for tracing snowmelt water through the hydrologic cycle. The purpose of this Briefing is to show the potential advantages of PCSs and recommend guidelines for constructing and installing them based on our preliminary results from two snowmelt seasons.

  4. Mineralogy and stable isotope geochemistry of hydrothermally altered oceanic rocks

    Science.gov (United States)

    Stakes, D.S.; O'Neil, J.R.

    1982-01-01

    Mineralogical and isotopic variations observed in altered glassy and crystalline rocks from the East Pacific Rise and the Mid-Atlantic Ridge provide information about the temperatures of alteration and seawater/rock ratios for various hydrothermal regimes within the oceanic crust. A systematic increase in alteration temperature is evident for the glassy rocks in the sequence: (1) nontronite and celadonite vesicle fillings (35??C), (2) saponite-rich pillow breccias (130-170??C), (3) calcite-rich greenstone breccias and epidote-rich greenstone (200-350??C). These results include the highest temperatures thus far reported for saponite formation. The "seawater-dominated" hydrothermal alteration process that formed the saponite-rich pillow breccias is characterized by high water/rock ratios (>50:1), low to moderate temperatures, a seawater origin of most of the carbon in vein calcites (??13 C ??? 0) and the predominance of Fe-rich saponite and calcite as secondary phases. Greenstones (chlorite-quartz-epidote) and greenstone breccias (chlorite-quartz-albite-calcite) are altered in a "rock-dominated" system with lower water/rock ratios (50:1 to isotopic composition to alter the overlying glassy rocks to the observed mineralogies as well as being the source of metal-rich deposits associated with the oceanic spreading centers. ?? 1982.

  5. Stable isotope sales: Mound Laboratory customer and shipment summaries, FY 1976 and FY 1976A

    Energy Technology Data Exchange (ETDEWEB)

    Ruwe, A.H. Jr. (comp.)

    1977-06-06

    A listing is given of Mound Laboratory's sales of stable isotopes of noble gases, carbon, oxygen, nitrogen, chlorine, and sulfur for fiscal years 1976 and 1976A (the period July 1, 1975 through September 30, 1976). Purchasers are listed alphabetically and are divided into domestic and foreign groups. A cross-reference index by location is included for domestic customers. Cross-reference listings by isotope purchased are included for all customers.

  6. Carbon stable isotope analysis of cereal remains as a way to reconstruct water availability: preliminary results

    OpenAIRE

    Flohr, Pascal; Muldner, Gundula; Jenkins, Emma

    2011-01-01

    Reconstructing past water availability, both as rainfall and irrigation, is important to answer questions about the way society reacts to climate and its changes and the role of irrigation in the development of social complexity. Carbon stable isotope analysis of archaeobotanical remains is a potentially valuable method for reconstructing water availability. To further define the relationship between water availability and plant carbon isotope composition and to set up baseline values for the...

  7. An introduction to stable water isotopes in climate models: benefits of forward proxy modelling for paleoclimatology

    Directory of Open Access Journals (Sweden)

    C. Sturm

    2009-06-01

    Full Text Available Stable water isotopes have been measured in a wide range of climate archives, with the purpose of reconstructing regional climate variations. Yet the common assumption that the isotopic signal is a direct indicator of temperature proves to be misleading under certain circumstances, since its relationship with temperature also depends on e.g. atmospheric circulation and precipitation seasonality. The present article introduces the principles, benefits and caveats of using climate models with embedded water isotopes as a support for the interpretation of isotopic climate archives. A short overview of the limitations of empirical calibrations of isotopic proxy records is presented, with emphasis on the physical processes that infirm its underlying hypotheses. The simulation of climate and its associated isotopic signal, despite difficulties related to downscaling and intrinsic atmospheric variability, can provide a "transfer function" between the isotopic signal and the considered climate variable. The multi-proxy data can then be combined with model output to produce a physically consistent climate reconstruction and its confidence interval. A sensitivity study with the isotope-enabled global circulation model CAM3iso under idealised present-day, pre-industrial and mid-Holocene is presented to illustrate the impact of a changing climate on the isotope-temperature relationship.

  8. Stable isotope analysis of precipitation samples obtained via crowdsourcing reveals the spatiotemporal evolution of Superstorm Sandy.

    Directory of Open Access Journals (Sweden)

    Stephen P Good

    Full Text Available Extra-tropical cyclones, such as 2012 Superstorm Sandy, pose a significant climatic threat to the northeastern United Sates, yet prediction of hydrologic and thermodynamic processes within such systems is complicated by their interaction with mid-latitude water patterns as they move poleward. Fortunately, the evolution of these systems is also recorded in the stable isotope ratios of storm-associated precipitation and water vapor, and isotopic analysis provides constraints on difficult-to-observe cyclone dynamics. During Superstorm Sandy, a unique crowdsourced approach enabled 685 precipitation samples to be obtained for oxygen and hydrogen isotopic analysis, constituting the largest isotopic sampling of a synoptic-scale system to date. Isotopically, these waters span an enormous range of values (> 21‰ for δ(18O, > 160‰ for δ(2H and exhibit strong spatiotemporal structure. Low isotope ratios occurred predominantly in the west and south quadrants of the storm, indicating robust isotopic distillation that tracked the intensity of the storm's warm core. Elevated values of deuterium-excess (> 25‰ were found primarily in the New England region after Sandy made landfall. Isotope mass balance calculations and Lagrangian back-trajectory analysis suggest that these samples reflect the moistening of dry continental air entrained from a mid-latitude trough. These results demonstrate the power of rapid-response isotope monitoring to elucidate the structure and dynamics of water cycling within synoptic-scale systems and improve our understanding of storm evolution, hydroclimatological impacts, and paleo-storm proxies.

  9. Stable isotope-labeling studies in metabolomics: new insights into structure and dynamics of metabolic networks.

    Science.gov (United States)

    Chokkathukalam, Achuthanunni; Kim, Dong-Hyun; Barrett, Michael P; Breitling, Rainer; Creek, Darren J

    2014-02-01

    The rapid emergence of metabolomics has enabled system-wide measurements of metabolites in various organisms. However, advances in the mechanistic understanding of metabolic networks remain limited, as most metabolomics studies cannot routinely provide accurate metabolite identification, absolute quantification and flux measurement. Stable isotope labeling offers opportunities to overcome these limitations. Here we describe some current approaches to stable isotope-labeled metabolomics and provide examples of the significant impact that these studies have had on our understanding of cellular metabolism. Furthermore, we discuss recently developed software solutions for the analysis of stable isotope-labeled metabolomics data and propose the bioinformatics solutions that will pave the way for the broader application and optimal interpretation of system-scale labeling studies in metabolomics.

  10. Stable isotope-labeled excipients for drug product identification and counterfeit detection.

    Science.gov (United States)

    Felton, Linda A; Shah, Punit P; Sharp, Zachary; Atudorei, Viorel; Timmins, Graham S

    2011-01-01

    Counterfeit drug products have become a major problem worldwide and a number of techniques to detect counterfeit products or reduce the potential for counterfeiting have been investigated. This study examined the use of stable isotope-labeled excipients in solid dosage forms as a method to identify drug products and to detect counterfeits. (2)H- and (13)C-glucose were used as model excipients and incorporated in wet granulated formulations at a variety of different isotopic ratios. The ratios of (2)H/(1)H and (13)C/(12)C in each product were then determined by isotope ratio mass spectrometry. Results demonstrated the ability to detect the isotope-labeled glucose in both granules and tablets. It was possible to use the isotope ratios to differentiate between specific batches of granules, demonstrating the potential of this technique for in-product, batch-specific identification.

  11. Analytical modelling of stable isotope fractionation of volatile organic compounds in the unsaturated zone

    CERN Document Server

    Bouchard, D; Höhener, P; Hunkeler, D; 10.1016/j.jconhyd.2010.09.006

    2011-01-01

    Analytical models were developed that simulate stable isotope ratios of volatile organic compounds (VOCs) near a point source contamination in the unsaturated zone. The models describe diffusive transport of VOCs, biodegradation and source ageing. The mass transport is governed by Fick's law for diffusion, and the equation for reactive transport of VOCs in the soil gas phase was solved for different source geometries and for different boundary conditions. Model results were compared to experimental data from a one-dimensional laboratory column and a radial-symmetric field experiment, and the comparison yielded a satisfying agreement. The model results clearly illustrate the significant isotope fractionation by gas-phase diffusion under transient state conditions. This leads to an initial depletion of heavy isotopes with increasing distance from the source. The isotope evolution of the source is governed by the combined effects of isotope fractionation due to vaporization, diffusion and biodegradation. The net...

  12. Controls on the stable isotope compositions of travertine from hyperalkaline springs in Oman: Insights from clumped isotope measurements

    Science.gov (United States)

    Falk, E. S.; Guo, W.; Paukert, A. N.; Matter, J. M.; Mervine, E. M.; Kelemen, P. B.

    2016-11-01

    Carbonate formation at hyperalkaline springs is typical of serpentinization in peridotite massifs worldwide. These travertines have long been known to exhibit large variations in their carbon and oxygen isotope compositions, extending from apparent equilibrium values to highly depleted values. However, the exact causes of these variations are not well constrained. We analyzed a suite of well-characterized fresh carbonate precipitates and travertines associated with hyperalkaline springs in the peridotite section of the Samail ophiolite, Sultanate of Oman, and found their clumped isotope compositions vary systematically with formation environments. Based on these findings, we identified four main processes controlling the stable isotope compositions of these carbonates. These include hydroxylation of CO2, partial isotope equilibration of dissolved inorganic carbon, mixing between isotopically distinct carbonate end-members, and post-depositional recrystallization. Most notably, in fresh crystalline films on the surface of hyperalkaline springs and in some fresh carbonate precipitates from the bottom of hyperalkaline pools, we observed large enrichments in Δ47 (up to ∼0.2‰ above expected equilibrium values) which accompany depletions in δ18O and δ13C, yielding about 0.01‰ increase in Δ47 and 1.1‰ decrease in δ13C for every 1‰ decrease in δ18O, relative to expected equilibrium values. This disequilibrium trend, also reflected in preserved travertines ranging in age from modern to ∼40,000 years old, is interpreted to arise mainly from the isotope effects associated with the hydroxylation of CO2 in high-pH fluids and agrees with our first-order theoretical estimation. In addition, in some fresh carbonate precipitates from the bottom of hyperalkaline pools and in subsamples of one preserved travertine terrace, we observed additional enrichments in Δ47 at intermediate δ13C and δ18O, consistent with mixing between isotopically distinct carbonate end

  13. Stable isotope ratios and reforestation potential in Acacia koa populations on Hawai’i

    Directory of Open Access Journals (Sweden)

    Shaneka Lawson

    2014-01-01

    Full Text Available Stable carbon and nitrogen isotopes can be influenced by a multitude of factors including elevation, precipitation rate, season, and temperature. This work examined the variability in foliar stable carbon (δ13C and nitrogen (δ15N isotope ratios of koa (Acacia koa for 17 sites on Hawai’i Island delineated by elevation and precipitation gradients. Sites were identified and grouped with respect to mean annual precipitation (MAP, mean annual temperature (MAT and position along three elevation ranges. Analysis of the resultant δ13C and δ15N isotope ratios from multiple individuals at these sites indicated that certain sites showed a demonstrated correlation between carbon and/or nitrogen content, isotope ratios, precipitation, and elevation however many sites showed no correlation. We used publicly available temperature and moisture data to help eliminate confounding effects by climatic drivers and capture possible points of contention. At sites where the temperature, precipitation, and elevation data were not significantly different we compared stable isotope information to determine if additional variables could have contributed to the lack of more correlative data. Our results note several areas within the Waiakea Forest Reserve and Volcanoes National Park where, based on isotope results, reforestation efforts could be most successfully initiated.

  14. Platinum stable isotope ratio measurements by double-spike multiple collector ICPMS

    DEFF Research Database (Denmark)

    Creech, J.; Baker, J.; Handler, M.

    2013-01-01

    external element (Pb) doping to correct for instrumental mass bias and have identified relative Pt isotope differences of up to 10% from the reference values for this standard. The new isotopic composition of the IRMM-010 standard (Pt = 0.01289%, Pt = 0.7938%, Pt = 32.81%, Pt = 33.79%, Pt = 25.29% and Pt......We present a new technique for the precise determination of platinum (Pt) stable isotope ratios by multiple-collector inductively coupled plasma mass spectrometry (MC-ICPMS) using two different Pt double-spikes ( Pt-Pt and Pt-Pt). Results are expressed relative to the IRMM-010 Pt isotope standard......) can be obtained on Pt stable isotope ratios with either double-spike. Elemental doping tests reveal that double-spike corrected Pt stable isotope ratios are insensitive to the presence of relatively high (up to 10%) levels of matrix elements, although the Pt-Pt double-spike is affected by an isobaric...

  15. Pan-Arctic concentrations of mercury and stable isotope ratios of carbon (δ{sup 13}C) and nitrogen (δ{sup 15}N) in marine zooplankton

    Energy Technology Data Exchange (ETDEWEB)

    Pomerleau, Corinne, E-mail: corinne.pomerleau@umanitoba.ca [Centre for Earth Observation Science, University of Manitoba, Winnipeg, MB R3T 2N2 (Canada); Greenland Institute of Natural Resources, Kivioq 2, Nuuk 3900, Greenland (Denmark); Stern, Gary A.; Pućko, Monika [Centre for Earth Observation Science, University of Manitoba, Winnipeg, MB R3T 2N2 (Canada); Foster, Karen L. [Foster Environmental, Peterborough, ON K9J 8L2 (Canada); Macdonald, Robie W. [Institute of Ocean Sciences, Fisheries and Oceans Canada, Sidney, BC V8L 4B2 (Canada); Fortier, Louis [Québec-Océan, Département de Biologie, Université Laval, Québec, QC G1V 0A6 (Canada)

    2016-05-01

    Zooplankton play a central role in marine food webs, dictating the quantity and quality of energy available to upper trophic levels. They act as “keystone” species in transfer of mercury (Hg) up through the marine food chain. Here, we present the first Pan-Arctic overview of total and monomethylmercury concentrations (THg and MMHg) and stable isotope ratios of carbon (δ{sup 13}C) and nitrogen (δ{sup 15}N) in selected zooplankton species by assembling data collected between 1998 and 2012 from six arctic regions (Laptev Sea, Chukchi Sea, southeastern Beaufort Sea, Canadian Arctic Archipelago, Hudson Bay and northern Baffin Bay). MMHg concentrations in Calanus spp., Themisto spp. and Paraeuchaeta spp. were found to increase with higher δ{sup 15}N and lower δ{sup 13}C. The southern Beaufort Sea exhibited both the highest THg and MMHg concentrations. Biomagnification of MMHg between Calanus spp. and two of its known predators, Themisto spp. and Paraeuchaeta spp., was greatest in the southern Beaufort Sea. Our results show large geographical variations in Hg concentrations and isotopic signatures for individual species related to regional ecosystem features, such as varying water masses and freshwater inputs, and highlight the increased exposure to Hg in the marine food chain of the southern Beaufort Sea. - Highlights: • Assessment of Pan-Arctic variability in zooplankton Hg concentrations • Increased exposure to Hg in the marine food chain of the southern Beaufort Sea • Zooplankton plays a central role in the Hg pathway within Arctic marine food webs.

  16. Migration dynamics of clupeoids in the Schelde estuary: a stable isotope approach

    DEFF Research Database (Denmark)

    Guelinckx, J.; Maes, J.; De Brabandere, Loreto

    2006-01-01

    using C and N stable isotopes. Prior to this,  stomach contents were used to verify the isotopic differences between the food sources at the sampling stations. From May 2000 to April 2001 fish were collected monthly in the upper and lower estuary. Muscle tissue and stomach contents were analyzed for d13...... movement in the Schelde estuary. Seasonal movements of clupeoids in the Schelde estuary were analyzed by separating the temporal abundance patterns into migration groups based on their muscle isotopic composition. Immigration and emigration seem to occur continuously throughout the year. Most exchange...

  17. Stable Isotopes Provide Insight into Population Structure and Segregation in Eastern North Atlantic Sperm Whales

    DEFF Research Database (Denmark)

    Borrell, Asuncion; Velasquez Vacca, Adriana; Pinela, Ana M.

    2013-01-01

    highly mobile, shows indication of structuring in the eastern North Atlantic, an ocean basin in which a single population is believed to occur. To do so, we examined stable isotope values in sequential growth layer groups of teeth from individuals sampled in Denmark and NW Spain. In each layer we...... measured oxygen-isotope ratios (delta O-18) in the inorganic component (hydroxyapatite), and nitrogen and carbon isotope ratios (delta N-15: delta C-13) in the organic component (primarily collagenous). We found significant differences between Denmark and NW Spain in delta N-15 and delta O-18 values...

  18. A stable isotope approach and its application for identifying nitrate source and transformation process in water.

    Science.gov (United States)

    Xu, Shiguo; Kang, Pingping; Sun, Ya

    2016-01-01

    Nitrate contamination of water is a worldwide environmental problem. Recent studies have demonstrated that the nitrogen (N) and oxygen (O) isotopes of nitrate (NO3(-)) can be used to trace nitrogen dynamics including identifying nitrate sources and nitrogen transformation processes. This paper analyzes the current state of identifying nitrate sources and nitrogen transformation processes using N and O isotopes of nitrate. With regard to nitrate sources, δ(15)N-NO3(-) and δ(18)O-NO3(-) values typically vary between sources, allowing the sources to be isotopically fingerprinted. δ(15)N-NO3(-) is often effective at tracing NO(-)3 sources from areas with different land use. δ(18)O-NO3(-) is more useful to identify NO3(-) from atmospheric sources. Isotopic data can be combined with statistical mixing models to quantify the relative contributions of NO3(-) from multiple delineated sources. With regard to N transformation processes, N and O isotopes of nitrate can be used to decipher the degree of nitrogen transformation by such processes as nitrification, assimilation, and denitrification. In some cases, however, isotopic fractionation may alter the isotopic fingerprint associated with the delineated NO3(-) source(s). This problem may be addressed by combining the N and O isotopic data with other types of, including the concentration of selected conservative elements, e.g., chloride (Cl(-)), boron isotope (δ(11)B), and sulfur isotope (δ(35)S) data. Future studies should focus on improving stable isotope mixing models and furthering our understanding of isotopic fractionation by conducting laboratory and field experiments in different environments.

  19. Estimating groundwater exchange with lakes: 1. The stable isotope mass balance method

    Science.gov (United States)

    Krabbenhoft, David P.; Bowser, Carl J.; Anderson, Mary P.; Valley, John W.

    1990-01-01

    Groundwater inflow and outflow contributions to the hydrologic budget of lakes can be determined using a stable isotope (18O/16O) mass balance method. The stable isotope method provides a way of integrating the spatial and temporal complexities of the flow field around a lake, thereby offering an appealing alternative to the traditional time and labor intensive methods using seepage meters and an extensive piezometer network. In this paper the method is applied to a lake in northern Wisconsin, demonstrating that it can be successfully applied to lakes in the upper midwest where thousands of similar lakes exist. Inflow and outflow rates calculated for the Wisconsin lake using the isotope mass balance method are 29 and 54 cm/yr, respectively, which compare well to estimates, derived independently using a three-dimensional groundwater flow and solute transport model, of 20 and 50 cm/yr. Such a favorable comparison lends confidence to the use of the stable isotope method to estimate groundwater exchange with lakes. In addition, utilization of stable isotopes in studies of groundwater-lake systems lends insight into mixing processes occurring in the unsaturated zone and in the aquifer surrounding the lake and verifies assumed flow paths based on head measurements in piezometers.

  20. Beyond diet reconstruction: stable isotope applications to human physiology, health, and nutrition.

    Science.gov (United States)

    Reitsema, Laurie J

    2013-01-01

    Analysis of stable carbon and nitrogen isotopes from soft or mineralized tissues is a direct and widely-used technique for modeling diets. In addition to its continued role in paleodiet analysis, stable isotope analysis is now contributing to studies of physiology, disease, and nutrition in archaeological and living human populations. In humans and other animals, dietary uptake and distribution of carbon and nitrogen among mineralized and soft tissue is carried out with varying efficiency due to factors of internal biology. Human pathophysiologies may lead to pathology-influenced isotopic fractionation that can be exploited to understand not just skeletal health and diet, but physiological health and nutrition. This study reviews examples from human biology, non-human animal ecology, biomedicine, and bioarchaeology demonstrating how stable isotope analyses are usefully applied to the study of physiological adaptation and adaptability. Suggestions are made for future directions in applying stable isotope analysis to the study of nutritional stress, disease, and growth and development in living and past human populations.

  1. Influence of regional precipitation patterns on stable isotopes in ice cores from the central Himalayas

    Directory of Open Access Journals (Sweden)

    H. Pang

    2013-05-01

    Full Text Available Several ice cores have been recovered from the Dasuopu Glacier and the East Rongbuk (ER Glacier in the central Himalayas since the 1990s. Although the distance between the ER and the Dasuopu ice core drilling sites is only ∼125 km, the stable isotopic record (δ18O or δD of the ER core is interpreted as a precipitation proxy while the Dasuopu core as a temperature proxy. Thus, the climatological significance of the stable isotopic records of these Himalayan ice cores remains a subject of debate. Based on analysis of regional precipitation patterns over the region, we find that the different interpretations of the Dasuopu and Everest isotopic records may not be contradictive. The north–south and west–east seesaws of the Indian Summer Monsoon (ISM precipitation are primarily responsible for precipitation falling at the ER site, which results in a negative correlation between the ER δ18O or δD record and precipitation amount along the southern slope of the central Himalayas, corresponding to the "amount effect". In addition to the ISM precipitation, non-summer monsoonal precipitation associated with winter westerlies also significantly contributes to precipitation falling at the Dasuopu site, which may cause a positive correlation between the Dasuopu stable isotopic record and temperature, in response to the "temperature effect". Our results have important implications for interpreting the stable isotopic ice core records recovered from different climatological regimes of the Himalayas.

  2. Recent developments in application of stable isotope analysis on agro-product authenticity and traceability.

    Science.gov (United States)

    Zhao, Yan; Zhang, Bin; Chen, Gang; Chen, Ailiang; Yang, Shuming; Ye, Zhihua

    2014-02-15

    With the globalisation of agro-product markets and convenient transportation of food across countries and continents, the potential for distribution of mis-labelled products increases accordingly, highlighting the need for measures to identify the origin of food. High quality food with identified geographic origin is a concern not only for consumers, but also for agriculture farmers, retailers and administrative authorities. Currently, stable isotope ratio analysis in combination with other chemical methods gradually becomes a promising approach for agro-product authenticity and traceability. In the last five years, a growing number of research papers have been published on tracing agro-products by stable isotope ratio analysis and techniques combining with other instruments. In these reports, the global variety of stable isotope compositions has been investigated, including light elements such as C, N, H, O and S, and heavy isotopes variation such as Sr and B. Several factors also have been considered, including the latitude, altitude, evaporation and climate conditions. In the present paper, an overview is provided on the authenticity and traceability of the agro-products from both animal and plant sources by stable isotope ratio analysis.

  3. Use of Stable Isotopes to Investigate Keratin Deposition in the Claw Tips of Ducks

    Science.gov (United States)

    Hopkins, John B.; Cutting, Kyle A.; Warren, Jeffrey M.

    2013-01-01

    Stable isotopes derived from the claws of birds could be used to determine the migratory origins of birds if the time periods represented in excised sections of claws were known. We investigated new keratin growth in the claws of adult female Lesser Scaup (Aythya affinis) by estimating the equilibration rates of stable isotopes (δ13C, δ15N, and δ2H) from the breeding grounds into 1 mm claw tips. We sampled birds on their breeding ground through time and found that it took approximately 3–3.5 months for isotope values in most claw tips to equilibrate to isotope values that reflected those present in the environment on their breeding grounds. Results from this study suggest that isotopes equilibrate slowly into claw tips of Lesser Scaup, suggesting isotopes could potentially be used to determine the wintering grounds of birds. We suggest using controlled feeding experiments or longitudinal field investigations to understand claw growth and isotopic equilibration in claw tips. Such information would be valuable in ascertaining whether claw tips can be used in future studies to identify the migratory origins of birds. PMID:24282563

  4. Stable isotopes as tracers of residency for fish on inshore coral reefs

    Science.gov (United States)

    Davis, Jean P.; Pitt, Kylie A.; Fry, Brian; Connolly, Rod M.

    2015-12-01

    Understanding the migratory movements of fish between habitats is an important priority for fisheries management. Carbon (C) and nitrogen (N) stable isotopes were used to evaluate the degree of movement and residency for five fish species collected from coral reefs in Queensland, Australia. Isotope values of fish were measured and compared between slow-turnover muscle tissue and fast-turnover liver tissue, with isotopic agreement between liver and muscle generally indicating resident animals, and relatively low C isotope values in muscle indicating migrants. Three fish species, rabbitfish (Siganus fuscescens), painted sweetlips (Diagramma labiosum) and Guenther's wrasse (Pseudolabrus guentheri) showed relatively consistent carbon isotope values between muscle and liver tissue as expected for resident populations. One quarter of bream (Acanthopagrus australis) individuals showed much lower δ13C values in muscle than liver. These low values diverged from the -10 to -15‰ values of residents and were more similar to the -20‰ values of fish collected from coastal riverine habitats, the presumed migration source. Moses perch (Lutjanus russelli) also showed substantial differences between muscle and liver C isotopes for about a quarter of individuals, but the overall higher C values of these individuals indicated they may have switched diets within island habitats rather than migrating. Our results were consistent with previous studies of fish residency and indicate that measuring stable isotopes in multiple tissues provides a useful methodology for characterizing fish residency in inshore areas.

  5. National uses and needs for separated stable isotopes in physics, chemistry, and geoscience research

    Science.gov (United States)

    Zisman, M. S.

    Present uses of separated stable isotopes in the fields of physics, chemistry, and the geosciences were surveyed to identify current supply problems and to determine future needs. Demand for separated isotopes remains strong, with 220 different nuclides having been used in the past three years. The largest needs, in terms of both quantity and variety of isotopes, are found in nuclear physics research. Current problems include a lack of availability of many nuclides, unsatisfactory enrichment of rare species, and prohibitively high costs for certain important isotopes. Demand for separated isotopes is expected to remain roughly at present levels, although a shift toward more requests for highly enriched rare isotopes is predicted. Use of neutron rich nuclides below A = 100 for producing exotic ion beams at various accelerators and use of transition metal nuclei for nuclear magnetic resonance spectroscopy are expected to expand. An increase in the need for calibration standards for techniques of radiological dating, such as Sm/Nd and Lu/Hf is predicted, but in relatively small quantities. Most members of the research community would be willing to pay considerably more than they do now to maintain adequate supplies of stable isotopes.

  6. National uses and needs for separated stable isotopes in physics, chemistry, and geoscience research

    Energy Technology Data Exchange (ETDEWEB)

    Zisman, M.S.

    1982-01-01

    Present uses of separated stable isotopes in the fields of physics, chemistry, and the geosciences have been surveyed to identify current supply problems and to determine future needs. Demand for separated isotopes remains strong, with 220 different nuclides having been used in the past three years. The largest needs, in terms of both quantity and variety of isotopes, are found in nuclear physics research. Current problems include a lack of availability of many nuclides, unsatisfactory enrichment of rare species, and prohibitively high costs for certain important isotopes. It is expected that demands for separated isotopes will remain roughly at present levels, although there will be a shift toward more requests for highly enriched rare isotopes. Significantly greater use will be made of neutron-rich nuclides below A = 100 for producing exotic ion beams at various accelerators. Use of transition metal nuclei for nuclear magnetic resonance spectroscopy will expand. In addition, calibration standards will be required for the newer techniques of radiological dating, such as the Sm/Nd and Lu/Hf methods, but in relatively small quantities. Most members of the research community would be willing to pay considerably more than they do now to maintain adequate supplies of stable isotopes.

  7. Stable hydrogen and oxygen isotope ratios of bottled waters of the world.

    Science.gov (United States)

    Bowen, Gabriel J; Winter, David A; Spero, Howard J; Zierenberg, Robert A; Reeder, Mathew D; Cerling, Thure E; Ehleringer, James R

    2005-01-01

    Bottled and packaged waters are an increasingly significant component of the human diet. These products are regulated at the regional, national, and international levels, and determining the authenticity of marketing and labeling claims represents a challenge to regulatory agencies. Here, we present a dataset of stable isotope ratios for bottled waters sampled worldwide, and consider potential applications of such data for regulatory, forensic and geochemical standardization applications. The hydrogen and oxygen isotope ratios of 234 samples of bottled water range from -147 per thousand to +15 per thousand and from -19.1 per thousand to +3.0 per thousand, respectively. These values fall within and span most of the normal range for meteoric waters, indicating that these commercially available products represent a source of waters for use as laboratory working standards in applications requiring standardization over a large range of isotope ratios. The measured values of bottled water samples cluster along the global meteoric water line, suggesting that bottled water isotope ratios preserve information about the water sources from which they were derived. Using the dataset, we demonstrate how bottled water isotope ratios provide evidence for substantial evaporative enrichment of water sources prior to bottling and for the marketing of waters derived from mountain and lowland sources under the same name. Comparison of bottled water isotope ratios with natural environmental water isotope ratios demonstrates that on average the isotopic composition of bottled water tends to be similar to the composition of naturally available local water sources, suggesting that in many cases bottled water need not be considered as an isotopically distinct component of the human diet. Our findings suggest that stable isotope ratios of bottled water have the power to distinguish ultimate (e.g., recharge) and proximal (e.g., reservoir) sources of bottled water and constitute a potential

  8. Stable carbon isotopes in tree rings: the failure of uniformitarianism

    Science.gov (United States)

    McCarroll, Danny

    2010-05-01

    When tree rings are used to reconstruct past climate we rely on the uniformitarian principle that ‘the present is the key to the past'. Relationships between measured parameters and climate that can be calibrated and verified over the instrumental period are assumed to be applicable at longer timescales. In the case of δ13C, however, the uniformitarian principle fails for two reasons. (1) The instrumental calibration period is also the period of anthropogenic increase in atmospheric CO2. δ13C is a function of the ratio of internal to ambient CO2, so maintaining constant δ13C over the industrial period requires an active plastic response, either restricting stomatal conductance or increasing assimilation rate. In some areas trees may have reached the limits of their plasticity so that over the last few decades δ13C values have been declining, independent of any changes in climate. If no correction is made, the recent response to climate will be a poor indicator of behaviour in the past. (2) Tree ring δ13C is often used to reconstruct past temperatures even though temperature rarely has a strong direct control over fractionation. The link is therefore via either sunshine or humidity, which over the calibration period may be very strongly correlated with temperature. Long isotope chronologies, when compared with independent evidence of past temperatures, however, can show periods of marked divergence. The strong covariance of temperature, sunshine and humidity over the last century may not have persisted over longer timescales with larger climatic perturbations. In the case of carbon isotopes the key to the past is not statistical inference based on recent behaviour, but a clear mechanistic understanding of the influence of climate and other factors on fractionation.

  9. Spectral analysis software improves confidence in plant and soil water stable isotope analyses performed by isotope ratio infrared spectroscopy (IRIS).

    Science.gov (United States)

    West, A G; Goldsmith, G R; Matimati, I; Dawson, T E

    2011-08-30

    Previous studies have demonstrated the potential for large errors to occur when analyzing waters containing organic contaminants using isotope ratio infrared spectroscopy (IRIS). In an attempt to address this problem, IRIS manufacturers now provide post-processing spectral analysis software capable of identifying samples with the types of spectral interference that compromises their stable isotope analysis. Here we report two independent tests of this post-processing spectral analysis software on two IRIS systems, OA-ICOS (Los Gatos Research Inc.) and WS-CRDS (Picarro Inc.). Following a similar methodology to a previous study, we cryogenically extracted plant leaf water and soil water and measured the δ(2)H and δ(18)O values of identical samples by isotope ratio mass spectrometry (IRMS) and IRIS. As an additional test, we analyzed plant stem waters and tap waters by IRMS and IRIS in an independent laboratory. For all tests we assumed that the IRMS value represented the "true" value against which we could compare the stable isotope results from the IRIS methods. Samples showing significant deviations from the IRMS value (>2σ) were considered to be contaminated and representative of spectral interference in the IRIS measurement. Over the two studies, 83% of plant species were considered contaminated on OA-ICOS and 58% on WS-CRDS. Post-analysis, spectra were analyzed using the manufacturer's spectral analysis software, in order to see if the software correctly identified contaminated samples. In our tests the software performed well, identifying all the samples with major errors. However, some false negatives indicate that user evaluation and testing of the software are necessary. Repeat sampling of plants showed considerable variation in the discrepancies between IRIS and IRMS. As such, we recommend that spectral analysis of IRIS data must be incorporated into standard post-processing routines. Furthermore, we suggest that the results from spectral analysis be

  10. Stable Carbon Isotope Ratios of Phenolic Compounds in Secondary Particulate Organic Matter Formed by Photooxidation of Toluene

    CERN Document Server

    Irei, Satoshi; Huang, Lin; Auld, Janeen; Collin, Fabrice; Hastie, Donald

    2014-01-01

    Compound-specific stable carbon isotope ratios for phenolic compounds in secondary particulate organic matter (POM) formed by photooxidation of toluene were studied. Secondary POM generated by photooxidation of toluene using a continuous-flow reactor and an 8 cubic meter indoor smog chamber was collected, and then extracted with acetonitrile. Eight phenolic compounds were identified in the extracts by a gas chromatograph coupled with a mass spectrometer, and their compound-specific stable carbon isotope ratios were determined by a gas chromatograph coupled with a combustion furnace followed by an isotope ratio mass spectrometer. The majority of the products, including methylnitrophenols and methylnitrocatechols, were isotopically depleted by 5 to 6 permil compared to the initial isotope ratio for toluene, whereas the isotope ratio for 4_nitrophenol remained the same as the initial isotope ratio for toluene. Based on the reaction mechanisms postulated in literature, stable carbon isotope ratios of these produc...

  11. Stable isotope fractionation analysis as a tool to monitor biodegradation in contaminated acquifers

    Science.gov (United States)

    Meckenstock, Rainer U.; Morasch, Barbara; Griebler, Christian; Richnow, Hans H.

    2004-12-01

    The assessment of biodegradation in contaminated aquifers has become an issue of increasing importance in the recent years. To some extent, this can be related to the acceptance of intrinsic bioremediation or monitored natural attenuation as a means to manage contaminated sites. Among the few existing methods to detect biodegradation in the subsurface, stable isotope fractionation analysis (SIFA) is one of the most promising approaches which is pronounced by the drastically increasing number of applications. This review covers the recent laboratory and field studies assessing biodegradation of contaminants via stable isotope analysis. Stable isotope enrichment factors have been found that vary from no fractionation for dioxygenase reactions converting aromatic hydrocarbons over moderate fractionation by monooxygenase reactions ( ɛ=-3‰) and some anaerobic studies on microbial degradation of aromatic hydrocarbons ( ɛ=-1.7‰) to larger fractionations by anaerobic dehalogenation reactions of chlorinated solvents ( ɛ=between -5‰ and -30‰). The different isotope enrichment factors can be related to the respective biochemical reactions. Based on that knowledge, we discuss under what circumstances SIFA can be used for a qualitative or even a quantitative assessment of biodegradation in the environment. In a steadily increasing number of cases, it was possible to explain biodegradation processes in the field based on isotope enrichment factors obtained from laboratory experiments with pure cultures and measured isotope values from the field. The review will focus on the aerobic and anaerobic degradation of aromatic hydrocarbons and chlorinated solvents as the major contaminants of groundwater. Advances in the instrumental development for stable isotope analysis are only mentioned if it is important for the understanding of the application.

  12. Separation of polybrominated diphenyl ethers in fish for compound-specific stable carbon isotope analysis

    Energy Technology Data Exchange (ETDEWEB)

    Zeng, Yan-Hong [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China); Graduate University of Chinese Academy of Sciences, Beijing, 100049 (China); Luo, Xiao-Jun, E-mail: luoxiaoj@gig.ac.cn [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China); Chen, Hua-Shan; Wu, Jiang-Ping; Chen, She-Jun; Mai, Bi-Xian [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China)

    2012-05-15

    A separation and isotopic analysis method was developed to accurately measure the stable carbon isotope ratios of polybrominated diphenyl ethers (PBDEs) with three to six substituted bromine atoms in fish samples. Sample extracts were treated with concentrated sulfuric acid to remove lipids, purified using complex silica gel column chromatography, and finally processed using alumina/silica (Al/Si) gel column chromatography. The purities of extracts were verified by gas chromatography and mass spectrometry (GC-MS) in the full-scan mode. The average recoveries of all compounds across the purification method were between 60% and 110%, with the exception of BDE-154. The stable carbon isotopic compositions of PBDEs can be measured with a standard deviation of less than 0.5 Per-Mille-Sign . No significant isotopic fraction was found during the purification of the main PBDE congeners. A significant change in the stable carbon isotope ratio of BDE-47 was observed in fish carcasses compared to the original isotopic signatures, implying that PBDE stable carbon isotopic compositions can be used to trace the biotransformation of PBDEs in biota. - Highlights: Black-Right-Pointing-Pointer A method for the purification of PBDEs for CSIA was developed. Black-Right-Pointing-Pointer The {delta}{sup 13}C of PBDE congeners can be measured with a standard deviation of less than 0.5 Per-Mille-Sign . Black-Right-Pointing-Pointer Common carp were exposed to a PBDE mixture to investigate debromination. Black-Right-Pointing-Pointer Ratios of the {delta}{sup 13}C values can be used to trace the debromination of PBDE in fish.

  13. Characterization of bile acid metabolism in man using bile acids labeled with stable isotopes. [/sup 13/C

    Energy Technology Data Exchange (ETDEWEB)

    Hofmann, A.F. Klein, P.D.

    1977-01-01

    Bile acids labeled with stable isotopes in the steroid moiety can be used to characterize bile acid metabolism in man. Isotope dilution studies give information on pool size and input. Biotransformations are easily characterized. Stable isotopically labeled bile acids offer the advantage of freedom from radiation hazard, and also offer the possibility of monitoring all pools simultaneously, since all bile acids are separated by gas chromatography before isotope measurements are made. Further, since the proportion of the pool labeled with stable isotopes is greater than that achieved when radioactive isotopes are used, stable isotopes may permit isotope dilution studies to be done on serum samples in which the absolute concentration of bile acids is very low. A major disadvantage is the complex technology required for stable isotope measurement which often makes remote processing necessary. Bile acid labeled with /sup 13/C in the amino acid moiety, e.g. cholylglycine-1-/sup 13/C can be used for detection of increased bile acid deconjugation by intestinal bacteria, since the glycine-/sup 13/C, when liberated, is rapidly converted to /sup 13/CO/sub 2/, which is expired in breath. Bile acids labeled with stable isotopes may also be used for quantitation by inverse isotope dilution, but the technique is still in the development stage and seems unlikely to compete successfully with radioimmunoassay.

  14. A gas chromatograph/mass spectrometry method for determining isotopic distributions in organic compounds used in the chemical approach to stable isotope separation

    Energy Technology Data Exchange (ETDEWEB)

    Martinez, A.M.; Spall, W.D.; Smith, B.F.

    1990-01-01

    A variety of gas chromatograph/mass spectrometry (GC/MS) methods have been developed to resolve benzene, benzophenone, anthracene, fluorenone, and their respective stable isotope analogs from other components by gas chromatography. The ratio of stable isotope-labeled material to natural isotopic abundance compounds is determined from the mass spectra averaged across the chromatographic peak. Both total ion and selective ion chromatographic approaches were used for relative data and comparison. 9 refs., 11 tabs.

  15. Enhanced understanding of ectoparasite–host trophic linkages on coral reefs through stable isotope analysis

    Directory of Open Access Journals (Sweden)

    Amanda W.J. Demopoulos

    2015-04-01

    Full Text Available Parasitism, although the most common type of ecological interaction, is usually ignored in food web models and studies of trophic connectivity. Stable isotope analysis is widely used in assessing the flow of energy in ecological communities and thus is a potentially valuable tool in understanding the cryptic trophic relationships mediated by parasites. In an effort to assess the utility of stable isotope analysis in understanding the role of parasites in complex coral-reef trophic systems, we performed stable isotope analysis on three common Caribbean reef fish hosts and two kinds of ectoparasitic isopods: temporarily parasitic gnathiids (Gnathia marleyi and permanently parasitic cymothoids (Anilocra. To further track the transfer of fish-derived carbon (energy from parasites to parasite consumers, gnathiids from host fish were also fed to captive Pederson shrimp (Ancylomenes pedersoni for at least 1 month. Parasitic isopods had δ13C and δ15N values similar to their host, comparable with results from the small number of other host–parasite studies that have employed stable isotopes. Adult gnathiids were enriched in 15N and depleted in 13C relative to juvenile gnathiids, providing insights into the potential isotopic fractionation associated with blood-meal assimilation and subsequent metamorphosis. Gnathiid-fed Pedersen shrimp also had δ13C values consistent with their food source and enriched in 15N as predicted due to trophic fractionation. These results further indicate that stable isotopes can be an effective tool in deciphering cryptic feeding relationships involving parasites and their consumers, and the role of parasites and cleaners in carbon transfer in coral-reef ecosystems specifically.

  16. Enhanced understanding of ectoparasite: host trophic linkages on coral reefs through stable isotope analysis

    Science.gov (United States)

    Demopoulos, Amanda W. J.; Sikkel, Paul C.

    2015-01-01

    Parasitism, although the most common type of ecological interaction, is usually ignored in food web models and studies of trophic connectivity. Stable isotope analysis is widely used in assessing the flow of energy in ecological communities and thus is a potentially valuable tool in understanding the cryptic trophic relationships mediated by parasites. In an effort to assess the utility of stable isotope analysis in understanding the role of parasites in complex coral-reef trophic systems, we performed stable isotope analysis on three common Caribbean reef fish hosts and two kinds of ectoparasitic isopods: temporarily parasitic gnathiids (Gnathia marleyi) and permanently parasitic cymothoids (Anilocra). To further track the transfer of fish-derived carbon (energy) from parasites to parasite consumers, gnathiids from host fish were also fed to captive Pederson shrimp (Ancylomenes pedersoni) for at least 1 month. Parasitic isopods had δ13C and δ15N values similar to their host, comparable with results from the small number of other host–parasite studies that have employed stable isotopes. Adult gnathiids were enriched in 15N and depleted in13C relative to juvenile gnathiids, providing insights into the potential isotopic fractionation associated with blood-meal assimilation and subsequent metamorphosis. Gnathiid-fed Pedersen shrimp also had δ13C values consistent with their food source and enriched in 15N as predicted due to trophic fractionation. These results further indicate that stable isotopes can be an effective tool in deciphering cryptic feeding relationships involving parasites and their consumers, and the role of parasites and cleaners in carbon transfer in coral-reef ecosystems specifically.

  17. Method for determination of stable carbon isotope ratio of methylnitrophenols in atmospheric PM

    Directory of Open Access Journals (Sweden)

    S. Moukhtar

    2011-05-01

    Full Text Available A technique for the measurement of the stable isotope ratio of methylnitrophenols in atmospheric particulate matter (PM is presented. It has been found in numerous laboratory studies that these compounds are photooxidation products of toluene in PM. Atmospheric samples from rural and suburban areas were collected for evaluation of the procedure. PM was collected on quartz fibre filters using dichotomous high volume air samplers for PM 2.5. Methylnitrophenols were extracted from the filters using acetonitrile. The sample was then purified using a combination of high-performance liquid chromatography (HPLC and solid phase extraction (SPE. The final solution was then divided into two aliquots. To one aliquot, a derivatising agent, Bis(trimethylsilyltrifluoroacetamide (BSTFA, was added to the solution for Gas Chromatography/Mass Spectroscopy (GC/MS analysis. The second half of the sample was stored at low temperature. When GC/MS analysis showed high enough concentrations the remaining sample was derivatized with BSTFA and analysed for stable isotope ratio using a Gas Chromatography/Isotope Ratio Mass Spectrometry (GC-IRMS.

    In all atmospheric PM samples analysed, 2-methyl-4-nitrophenol was found to be the most abundant methylnitrophenol. Nevertheless, due to low pollution levels occurring in the rural area, no samples had concentrations high enough to perform stable carbon isotope composition measurements of the methylnitrophenols. Samples collected in the suburban area could be analysed for carbon stable isotope ratio using GC-IRMS.

    The procedure described in this paper provides a very sensitive and selective method for the analysis of methylnitrophenols in atmospheric PM at concentrations as low as 1 pg m−3. For accurate (within ±0.5‰ stable isotope ratio analysis significantly higher concentrations in the range of 100 pg m−3 or more are required.

  18. Particular geoscientific perspectives on stable isotope analysis in the arboreal system

    Science.gov (United States)

    Helle, Gerhard; Balting, Daniel; Pauly, Maren; Slotta, Franziska

    2017-04-01

    In geosciences stable isotopes of carbon, oxygen and hydrogen from the tree ring archive have been used for several decades to trace the course of past environmental and climatological fluctuations. In contrast to ice cores, the tree ring archive is of biological nature (like many other terrestrial archives), but provides the opportunity to establish site networks with very high resolution in space and time. Many of the basic physical mechanisms of isotope shifts are known, but biologically mediated processes may lead to isotope effects that are poorly understood. This implies that the many processes within the arboreal system leading to archived isotope ratios in wood material are governed by a multitude of environmental variables that are not only tied to the isotopic composition of atmospheric source values (precipitation, CO2), but also to seasonally changing metabolic flux rates and pool sizes of photosynthates within the trees. Consequently, the extraction of climate and environmental information is particularly challenging and reconstructions are still of rather qualitative nature. Over the last 10 years or so, monitoring studies have been implemented to investigate stable isotope, climate and environmental signal transfer within the arboreal system to develop transfer or response functions that can translate the relevant isotope values extracted from tree rings into climate or other environmental variables. To what extent have these efforts lead to a better understanding that helps improving the meaningfulness of tree ring isotope signals? For example, do monitoring studies help deciphering the causes for age-related trends in tree ring stable isotope sequences that are published in a growing number of papers. Are existing monitoring studies going into detail enough or is it already too much effort for the outcome? Based on what we know already particularly in mesic habitats, tree ring stable isotopes are much better climate proxies than other tree ring

  19. Groundwater stable isotope profile of the Etosha National Park, Namibia

    Directory of Open Access Journals (Sweden)

    Edward S. Riddell

    2016-03-01

    Full Text Available The Etosha National Park (ENP is a large protected area in northern Namibia. While the ENP has received a lot of research attention in terms of terrestrial ecosystem process understanding in recent decades, aquatic and hydrological research has to date been limited to a descriptive form. This study provides a baseline hydrological data set of the spatial representation of Oand H-isotope ratios in the groundwater at a park scale, with a focus on three water point types utilised by game, namely natural artesian and contact springs as well as artificial boreholes. The data are used to infer broad-scale hydrological process from groundwater recharge mechanisms dominated by direct rainfall recharge in the west of the ENP to evaporative controls on surface water recharge pathways in the east of the ENP close to Fishers Pan. The findings are used to recommend further targeted research and monitoring to aid management of water resources in the ENP.Conservation implications: The terrestrial ecosystem, particularly large game, are tightly coupled to the distribution of available surface water in the ENP, notably contact and artesian springs. Within the ENP there is a perceived desiccation of these springs. This study provides a baseline upon which more comprehensive studies should be undertaken to differentiate natural from anthropogenic causes for this phenomenon.

  20. The Conflict between Cheetahs and Humans on Namibian Farmland Elucidated by Stable Isotope Diet Analysis

    Science.gov (United States)

    Voigt, Christian C.; Thalwitzer, Susanne; Melzheimer, Jörg; Blanc, Anne-Sophie; Jago, Mark; Wachter, Bettina

    2014-01-01

    Large areas of Namibia are covered by farmland, which is also used by game and predator species. Because it can cause conflicts with farmers when predators, such as cheetahs (Acinonyx jubatus), hunt livestock, we assessed whether livestock constitutes a significant part of the cheetah diet by analysing the stable isotope composition of blood and tissue samples of cheetahs and their potential prey species. According to isotopic similarities, we defined three isotopic categories of potential prey: members of a C4 food web with high δ15N values (gemsbok, cattle, springhare and guinea fowl) and those with low δ15N values (hartebeest, warthog), and members of a C3 food web, namely browsers (eland, kudu, springbok, steenbok and scrub hare). We quantified the trophic discrimination of heavy isotopes in cheetah muscle in 9 captive individuals and measured an enrichment for 15N (3.2‰) but not for 13C in relation to food. We captured 53 free-ranging cheetahs of which 23 were members of groups. Cheetahs of the same group were isotopically distinct from members of other groups, indicating that group members shared their prey. Solitary males (n = 21) and males in a bachelor groups (n = 11) fed mostly on hartebeest and warthogs, followed by browsers in case of solitary males, and by grazers with high δ15N values in case of bachelor groups. Female cheetahs (n = 9) predominantly fed on browsers and used also hartebeest and warthogs. Mixing models suggested that the isotopic prey category that included cattle was only important, if at all, for males living in bachelor groups. Stable isotope analysis of fur, muscle, red blood cells and blood plasma in 9 free-ranging cheetahs identified most individuals as isotopic specialists, focussing on isotopically distinct prey categories as their food. PMID:25162403

  1. The conflict between cheetahs and humans on Namibian farmland elucidated by stable isotope diet analysis.

    Directory of Open Access Journals (Sweden)

    Christian C Voigt

    Full Text Available Large areas of Namibia are covered by farmland, which is also used by game and predator species. Because it can cause conflicts with farmers when predators, such as cheetahs (Acinonyx jubatus, hunt livestock, we assessed whether livestock constitutes a significant part of the cheetah diet by analysing the stable isotope composition of blood and tissue samples of cheetahs and their potential prey species. According to isotopic similarities, we defined three isotopic categories of potential prey: members of a C4 food web with high δ15N values (gemsbok, cattle, springhare and guinea fowl and those with low δ15N values (hartebeest, warthog, and members of a C3 food web, namely browsers (eland, kudu, springbok, steenbok and scrub hare. We quantified the trophic discrimination of heavy isotopes in cheetah muscle in 9 captive individuals and measured an enrichment for 15N (3.2‰ but not for 13C in relation to food. We captured 53 free-ranging cheetahs of which 23 were members of groups. Cheetahs of the same group were isotopically distinct from members of other groups, indicating that group members shared their prey. Solitary males (n = 21 and males in a bachelor groups (n = 11 fed mostly on hartebeest and warthogs, followed by browsers in case of solitary males, and by grazers with high δ15N values in case of bachelor groups. Female cheetahs (n = 9 predominantly fed on browsers and used also hartebeest and warthogs. Mixing models suggested that the isotopic prey category that included cattle was only important, if at all, for males living in bachelor groups. Stable isotope analysis of fur, muscle, red blood cells and blood plasma in 9 free-ranging cheetahs identified most individuals as isotopic specialists, focussing on isotopically distinct prey categories as their food.

  2. The conflict between cheetahs and humans on Namibian farmland elucidated by stable isotope diet analysis.

    Science.gov (United States)

    Voigt, Christian C; Thalwitzer, Susanne; Melzheimer, Jörg; Blanc, Anne-Sophie; Jago, Mark; Wachter, Bettina

    2014-01-01

    Large areas of Namibia are covered by farmland, which is also used by game and predator species. Because it can cause conflicts with farmers when predators, such as cheetahs (Acinonyx jubatus), hunt livestock, we assessed whether livestock constitutes a significant part of the cheetah diet by analysing the stable isotope composition of blood and tissue samples of cheetahs and their potential prey species. According to isotopic similarities, we defined three isotopic categories of potential prey: members of a C4 food web with high δ15N values (gemsbok, cattle, springhare and guinea fowl) and those with low δ15N values (hartebeest, warthog), and members of a C3 food web, namely browsers (eland, kudu, springbok, steenbok and scrub hare). We quantified the trophic discrimination of heavy isotopes in cheetah muscle in 9 captive individuals and measured an enrichment for 15N (3.2‰) but not for 13C in relation to food. We captured 53 free-ranging cheetahs of which 23 were members of groups. Cheetahs of the same group were isotopically distinct from members of other groups, indicating that group members shared their prey. Solitary males (n = 21) and males in a bachelor groups (n = 11) fed mostly on hartebeest and warthogs, followed by browsers in case of solitary males, and by grazers with high δ15N values in case of bachelor groups. Female cheetahs (n = 9) predominantly fed on browsers and used also hartebeest and warthogs. Mixing models suggested that the isotopic prey category that included cattle was only important, if at all, for males living in bachelor groups. Stable isotope analysis of fur, muscle, red blood cells and blood plasma in 9 free-ranging cheetahs identified most individuals as isotopic specialists, focussing on isotopically distinct prey categories as their food.

  3. Utilizing Stable Isotopes and Isotopic Anomalies to Study Early Solar System Formation Processes

    Science.gov (United States)

    Simon, Justin

    2017-01-01

    Chondritic meteorites contain a diversity of particle components, i.e., chondrules and calcium-, aluminum-rich refractory inclusions (CAIs), that have survived since the formation of the Solar System. The chemical and isotopic compositions of these materials provide a record of the conditions present in the protoplanetary disk where they formed and can aid our understanding of the processes and reservoirs in which solids formed in the solar nebula, an important step leading to the accretion of planetesimals. Isotopic anomalies associated with nucleosynthetic processes are observed in these discrete materials, and can be compared to astronomical observations and astrophysical formation models of stars and more recently proplyds. The existence and size of these isotopic anomalies are typically thought to reflect a significant state of isotopic heterogeneity in the earliest Solar System, likely left over from molecular cloud heterogeneities on the grain scale, but some could also be due to late stellar injection. The homogenization of these isotopic anomalies towards planetary values can be used to track the efficiency and timescales of disk wide mixing,

  4. Mixing of water in a carbonate aquifer, southern Italy, analysed through stable isotope investigations

    Directory of Open Access Journals (Sweden)

    Petrella Emma

    2013-01-01

    Full Text Available Mixing of water was analysed in a carbonate aquifer, southern Italy, through stable isotope investigations (18O,δ2H. The input signal (rainwater was compared with the isotopic content of a 35-meter groundwater vertical prof ile, over a 1-year period. Within the studied aquifer, recharge and f low are diffuse in a well-connected f issure network.At the test site, the comparison between input and groundwater isotopic signals illustrates that no eff icient mixing takes place in the whole unsaturated zone, between the fresh inf iltration water and the stored water.When analysing the stable isotope composition of groundwater, signif icant variations were observed above the threshold elevation of 1062 m asl, while a nearly constant composition was observed below the same threshold. Thus, temporal variations in stable isotope composition of rainwater are completely attenuated just in the deeper phreatic zone.On the whole, taking into consideration also the results of previous studies in the same area, the investigations showed that physical characteristics of the carbonate bedrock, as well as aquifer heterogeneity, are factors of utmost importance in inf luencing the complete mixing of water. These f indings suggest a more complex scenario at catchment scale.

  5. Application of Stable Isotope-Assisted Metabolomics for Cell Metabolism Studies

    Directory of Open Access Journals (Sweden)

    Le You

    2014-03-01

    Full Text Available The applications of stable isotopes in metabolomics have facilitated the study of cell metabolisms. Stable isotope-assisted metabolomics requires: (1 properly designed tracer experiments; (2 stringent sampling and quenching protocols to minimize isotopic alternations; (3 efficient metabolite separations; (4 high resolution mass spectrometry to resolve overlapping peaks and background noises; and (5 data analysis methods and databases to decipher isotopic clusters over a broad m/z range (mass-to-charge ratio. This paper overviews mass spectrometry based techniques for precise determination of metabolites and their isotopologues. It also discusses applications of isotopic approaches to track substrate utilization, identify unknown metabolites and their chemical formulas, measure metabolite concentrations, determine putative metabolic pathways, and investigate microbial community populations and their carbon assimilation patterns. In addition, 13C-metabolite fingerprinting and metabolic models can be integrated to quantify carbon fluxes (enzyme reaction rates. The fluxome, in combination with other “omics” analyses, may give systems-level insights into regulatory mechanisms underlying gene functions. More importantly, 13C-tracer experiments significantly improve the potential of low-resolution gas chromatography-mass spectrometry (GC-MS for broad-scope metabolism studies. We foresee the isotope-assisted metabolomics to be an indispensable tool in industrial biotechnology, environmental microbiology, and medical research.

  6. Microbial degradation of alpha-cypermethrin in soil by compound-specific stable isotope analysis.

    Science.gov (United States)

    Xu, Zemin; Shen, Xiaoli; Zhang, Xi-Chang; Liu, Weiping; Yang, Fangxing

    2015-09-15

    To assess microbial degradation of alpha-cypermethrin in soil, attenuation of alpha-cypermethrin was investigated by compound-specific stable isotope analysis. The variations of the residual concentrations and stable carbon isotope ratios of alpha-cypermethrin were detected in unsterilized and sterilized soils spiked with alpha-cypermethrin. After an 80 days' incubation, the concentrations of alpha-cypermethrin decreased to 0.47 and 3.41 mg/kg in the unsterilized soils spiked with 2 and 10 mg/kg, while those decreased to 1.43 and 6.61 mg/kg in the sterilized soils. Meanwhile, the carbon isotope ratios shifted to -29.14 ± 0.22‰ and -29.86 ± 0.33‰ in the unsterilized soils spiked with 2 and 10 mg/kg, respectively. The results revealed that microbial degradation contributed to the attenuation of alpha-cypermethrin and induced the carbon isotope fractionation. In order to quantitatively assess microbial degradation, a relationship between carbon isotope ratios and residual concentrations of alpha-cypermethrin was established according to Rayleigh equation. An enrichment factor, ϵ = -1.87‰ was obtained, which can be employed to assess microbial degradation of alpha-cypermethrin. The significant carbon isotope fractionation during microbial degradation suggests that CSIA is a proper approach to qualitatively detect and quantitatively assess the biodegradation during attenuation process of alpha-cypermethrin in the field.

  7. Trophic structure in a seabird host-parasite food web: insights from stable isotope analyses.

    Directory of Open Access Journals (Sweden)

    Elena Gómez-Díaz

    Full Text Available Ecological studies on food webs rarely include parasites, partly due to the complexity and dimensionality of host-parasite interaction networks. Multiple co-occurring parasites can show different feeding strategies and thus lead to complex and cryptic trophic relationships, which are often difficult to disentangle by traditional methods. We analyzed stable isotope ratios of C ((13C/(12C, delta(13C and N ((15N/(14N, delta(15N of host and ectoparasite tissues to investigate trophic structure in 4 co-occurring ectoparasites: three lice and one flea species, on two closely related and spatially segregated seabird hosts (Calonectris shearwaters. delta(13C isotopic signatures confirmed feathers as the main food resource for the three lice species and blood for the flea species. All ectoparasite species showed a significant enrichment in delta(15N relatively to the host tissue consumed (discrimination factors ranged from 2 to 5 per thousand depending on the species. Isotopic differences were consistent across multiple host-ectoparasite locations, despite of some geographic variability in baseline isotopic levels. Our findings illustrate the influence of both ectoparasite and host trophic ecology in the isotopic structuring of the Calonectris ectoparasite community. This study highlights the potential of stable isotope analyses in disentangling the nature and complexity of trophic relationships in symbiotic systems.

  8. Application of Stable Isotope-Assisted Metabolomics for Cell Metabolism Studies

    Science.gov (United States)

    You, Le; Zhang, Baichen; Tang, Yinjie J.

    2014-01-01

    The applications of stable isotopes in metabolomics have facilitated the study of cell metabolisms. Stable isotope-assisted metabolomics requires: (1) properly designed tracer experiments; (2) stringent sampling and quenching protocols to minimize isotopic alternations; (3) efficient metabolite separations; (4) high resolution mass spectrometry to resolve overlapping peaks and background noises; and (5) data analysis methods and databases to decipher isotopic clusters over a broad m/z range (mass-to-charge ratio). This paper overviews mass spectrometry based techniques for precise determination of metabolites and their isotopologues. It also discusses applications of isotopic approaches to track substrate utilization, identify unknown metabolites and their chemical formulas, measure metabolite concentrations, determine putative metabolic pathways, and investigate microbial community populations and their carbon assimilation patterns. In addition, 13C-metabolite fingerprinting and metabolic models can be integrated to quantify carbon fluxes (enzyme reaction rates). The fluxome, in combination with other “omics” analyses, may give systems-level insights into regulatory mechanisms underlying gene functions. More importantly, 13C-tracer experiments significantly improve the potential of low-resolution gas chromatography-mass spectrometry (GC-MS) for broad-scope metabolism studies. We foresee the isotope-assisted metabolomics to be an indispensable tool in industrial biotechnology, environmental microbiology, and medical research. PMID:24957020

  9. Variation of the stable isotopes of water with altitude in the Saint Elias Mountains of Canada

    Science.gov (United States)

    Holdsworth, G.; Fogarasi, S.; Krouse, H. R.

    1991-04-01

    The stable isotopes of water, measured in melt samples taken from snow pits and cores at locations between 1750- and 5930-m altitude on Mount Logan (5951 m) and between 2900 and 4900 m on Mount Steele (5079 m), in the Saint Elias Mountains, Yukon, show a distinctive altitudinal distribution. Several delta O-18 and delta-D versus altitude profiles indicate the general persistence of a nearly iso-delta step, or staircase structure, separating a lower region of altitude-dependent isotopic fractionation between 1750 and 3350 m from another apparent fractionation sequence appearing above about 5300 m. Both of these sequences, but especially the lower one, indicate orderly processes. On the one hand, postdepositional changes to isotope ratios in snow at different altitudes may cause distortions to an otherwise nearly monotonic isotope fractionation sequence, but the main anomaly appears to be far too large to be explained in this way. On the other hand, an explanation linked to processes occurring in the lower and midtroposphere is based on established meteorological principles as well as on upper air data. This hypothesis is proposed as the primary one to explain the gross features of the observed isotope profiles. It is compatible with the concept of secondary-source moisture arriving via the upper troposphere, and it does not exclude the effects of postdepositional stratigraphic and stable isotope ratio changes.

  10. Photon strength distributions in stable even-even molybdenum isotopes

    CERN Document Server

    Wagner, A; Erhard, M; Grosse, E; Junghans, A R; Klug, J; Kosev, K; Nair, C; Nankov, N; Rusev, G; Schilling, K D; Schwengner, R

    2007-01-01

    Electromagnetic dipole-strength distributions up to the particle separation energies are studied for the stable even-even nuclides $^{92,94,96,98,100}$Mo in photon scattering experiments at the superconducting electron accelerator ELBE of the Forschungszentrum Dresden-Rossendorf. The influence of inelastic transitions to low-lying excited states has been corrected by a simulation of $\\gamma$ cascades using a statistical model. After corrections for branching ratios of ground-state transitions, the photon-scattering cross-sections smoothly connect to data obtained from $(\\gamma,n)$-reactions. With the newly determined electromagnetic dipole response of nuclei well below the particle separation energies the parametrisation of the isovector giant-dipole resonance is done with improved precision.

  11. Assessing anthropogenic pressures on groundwater using stable OH isotopes: perspectives and issues

    Science.gov (United States)

    Negrel, Philippe; Ollivier, Patrick; Flehoc, Christine; Hube, Daniel

    2015-04-01

    Large developments of isotope hydrogeology were done and well-established techniques mainly applying stable isotopes of the water molecule (hydrogen and oxygen) are now used largely to trace water provenance but also recharge processes. New methods allow the use of non-traditional isotopes (metals, compound specific stable isotope analysis CSIA...) to trace anthropogenic pressures in surface- and groundwater. Groundwater contamination in large industrial sites may come from several origins such as leakage from tanks during the production process of chemical products, liquid storage tanks, solid end product or past accumulated product in soil which is released over the time. The understanding of the origin and the further evolution of the chemical contamination in groundwater in an industrial site issued from past or current industrial activities is essential for the industrial companies regarding their environmental policies. The objective of this study was to use with an innovative way the stable isotopes of the water molecule as a low cost tool to trace pollutant plumes in groundwater and help to a better management of contaminated industrial sites. We present data on stable isotopes O and H in an European region where electrochemistry plants occur. For confidentiality purposes, the sites remain anonymous. Present day industrial activities have a direct impact on the groundwater over the site and migration of the contaminant(s) plume out of the site is supposed. We first characterize the natural groundwater background through the O-H characterization of surface water, lakes, thermal waters and regional shallow aquifers. High and low altitude recharge can be demonstrated in the area. Secondly, we used the stable isotope of the water molecule to trace over the site the impact of the Cl-rich liquor manufacturing process. Large deuterium enrichment was evidenced in the groundwater and the high values can be related to a direct contamination of the groundwater through

  12. Stable isotope evidence for increasing dietary breadth in the European mid-Upper Paleolithic.

    Science.gov (United States)

    Richards, M P; Pettitt, P B; Stiner, M C; Trinkaus, E

    2001-05-22

    New carbon and nitrogen stable isotope values for human remains dating to the mid-Upper Paleolithic in Europe indicate significant amounts of aquatic (fish, mollusks, and/or birds) foods in some of their diets. Most of this evidence points to exploitation of inland freshwater aquatic resources in particular. By contrast, European Neandertal collagen carbon and nitrogen stable isotope values do not indicate significant use of inland aquatic foods but instead show that they obtained the majority of their protein from terrestrial herbivores. In agreement with recent zooarcheological analyses, the isotope results indicate shifts toward a more broad-spectrum subsistence economy in inland Europe by the mid-Upper Paleolithic period, probably associated with significant population increases.

  13. Determination of the geographical origin of Chinese teas based on stable carbon and nitrogen isotope ratios

    Institute of Scientific and Technical Information of China (English)

    Long ZHANG; Jia-rong PAN; Cheng ZHU

    2012-01-01

    The objective of this study was to investigate the geographical origin of Chinese teas using carbon and nitrogen stable isotope ratio technology.The results showed that inter-provincial dispersion of teas in Guangdong (GD),Guangxi (GX),Hainan (HA),Fujian (F J),Shandong (SD),Sichuan (SC),Chongqing (CQ),and Henan (HN) provinces was high,while in Zhejiang (ZJ),Hubei (HB),Yunnan (YN),and Anhui (AH) provinces,it was low.Tea samples from GD,GX,HA,and FJ provinces were clustered in one group and separated from those from AH and HB provinces.Thus,carbon and nitrogen stable isotope ratio technology could discriminate teas from among some provinces of China,but not from among others.Better separation might be obtained with a combination of isotopic ratios and other indexes,such as elemental data and organic components.

  14. Emerging Techniques in Vegetable Oil Analysis Using Stable Isotope Ratio Mass Spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, S. D.; Rhodes, C.

    2002-07-01

    As the practice of vegetable oil adulteration becomes more sophisticated, the possibility to subvert detection using established techniques such as capillary gas chromatography is increasing. One of the most powerful techniques to be used in food authenticity studies is stable isotope ratio mass spectrometry (SIRMS) which utilises differences in the natural abundance of the stable isotopes of the light bio elements hydrogen, nitrogen, carbon, oxygen and sulfur to detect food fraud. SIRMS has found application in the authentication of a wide range of foodstuffs, including fruit juices, wines, spirits, honey and to detect the adulteration of flavour compounds with synthetic analogues. This papers reviews the current state-of-the-art for the authentication of vegetable oils using SIRMS and highlights emergent techniques such as compound-an position specific-isotope mass spectrometry. These latter developments offer the potential to provide more rapid and improved detection of the economic adulteration of vegetable oils. (Author) 38 refs.

  15. Identifying Neolithic animal management practices in the Adriatic using stable isotopes

    Directory of Open Access Journals (Sweden)

    Emily Zavodny

    2015-12-01

    Full Text Available We synthesise reported stable isotope values for domesticates and wild herbivores from sites spanning the Neolithic in coastal Croatia, Slovenia, and Italy (6000–3500 calBC. Carbon and nitrogen stable isotope values are analyzed as proxies of diet and environment, with differences between species possibly indicating anthropogenic influence. Results are used to characterise diets and address questions of the origin and development of husbandry strategies, especially transhumance, in early farming communities. Changes in pig carbon and nitrogen isotope values through time suggest alterations in practices, whereas values remain relatively constant for cattle and ovicaprids during most of the Neolithic, despite assumptions of seasonal mobility.

  16. You Are What You Eat: Mass spectrometry in paediatric kinetic studies using stable isotopes

    NARCIS (Netherlands)

    H. Schierbeek (Henk)

    2009-01-01

    textabstractAn overview will be presented about applications of stable isotopes in paediatric research. Mass spectrometry has proven to be an essential tool for unravelling kinetic studies in a large range of different research disciplines related to intestinal diseases, obesities, severe cerebral p

  17. Patterns in stable isotope ratios of particulate material from the eastern US continental shelf

    Science.gov (United States)

    Stable isotope measurements of nitrogen and carbon (δ15N, δ13C) in estuarine, nearshore, and open ocean ecosystems are often utilized in order to characterize human influences, elucidate food web dynamics, or better understand nitrogen cycling. Reliable information a...

  18. Stable isotope dilution analysis of orotic acid and uracil in amniotic fluid

    NARCIS (Netherlands)

    Jakobs, C.; Sweetman, L.; Nyhan, W.L.; Gruenke, L.; Craig, J.C.; Wadman, S.K.

    1984-01-01

    Rapid, sensitive and accurate stable isotope dilution assays were developed for the measurement of orotic acid and uracil in amniotic fluid. The method utilizes [15N2]orotic acid and [15N2]uracil as internal standards, isolation by liquid partition chromatography and quantitation by chemical ionizat

  19. Coral cavity sponges depend on reef-derived food resources: stable isotope and fatty acid constraints

    NARCIS (Netherlands)

    van Duyl, F.C.; Moodley, L.; Nieuwland, G.; van IJzerloo, L.; van Soest, R.W.M.; Houtekamer, M.; Meesters, E.H.; Middelburg, J.J.

    2011-01-01

    The diet of cavity sponges on the narrow fringing reefs of Curaçao, Caribbean was studied. The origin and resources of the bulk food of these sponges, i.e., dissolved organic matter (DOM), were identified using stable carbon and nitrogen isotopes and fatty acid biomarkers. We found that phytoplankto

  20. Coral cavity sponges depend on reef-derived food resources: stable isotope and fatty acid constraints

    NARCIS (Netherlands)

    van Duyl, F.C.; Moodley, L.; Nieuwland, G.; van Ijzerloo, L.; van Soest, R.W.M.; Houtekamer, M.; Meesters, E.H.; Middelburg, J.J.

    2011-01-01

    The diet of cavity sponges on the narrow fringing reefs of Cura double dagger ao, Caribbean was studied. The origin and resources of the bulk food of these sponges, i.e., dissolved organic matter (DOM), were identified using stable carbon and nitrogen isotopes and fatty acid biomarkers. We found tha

  1. Coral cavity sponges depend on reef-derived food resources: stable isotope and fatty acid constraints

    NARCIS (Netherlands)

    Duyl, F.C. van; Moodley, L.; Nieuwland, G.; IJzerloo, L. van; Soest, R.W.M. van; Houtekamer, M.; Meesters, E.H.; Middelburg, J.J.

    2011-01-01

    The diet of cavity sponges on the narrow fringing reefs of Curac¸ao, Caribbean was studied. The origin and resources of the bulk food of these sponges, i.e., dissolved organic matter (DOM), were identified using stable carbon and nitrogen isotopes and fatty acid biomarkers. We found that phytoplankt

  2. Patterns in stable isotope ratios of particulate material from the eastern US continental shelf

    Science.gov (United States)

    Stable isotope measurements of nitrogen and carbon (δ15N, δ13C) in estuarine, nearshore, and open ocean ecosystems are often utilized in order to characterize human influences, elucidate food web dynamics, or better understand nitrogen cycling. Reliable information a...

  3. Using Stable Isotopes of Carbon and Nitrogen to Evaluate Trophic Interactions in Aquatic Environments

    Science.gov (United States)

    Christensen, David R.; LaRoche, Andrew

    2012-01-01

    This paper describes a series of laboratory exercises for upper level biology courses, independent research and/or honors programs. Students sampled fish from a local water body with the assistance of a local fish and wildlife agency. Tissue samples from collected fish were utilized to obtain estimates of the stable isotopes delta[superscript 13]C…

  4. Noninvasive Imaging of Protein Metabolic Labeling in Single Human Cells Using Stable Isotopes and Raman Microscopy

    NARCIS (Netherlands)

    Manen, van Henk-Jan; Lenferink, Aufried; Otto, Cees

    2008-01-01

    We have combined nonresonant Raman microspectroscopy and spectral imaging with stable isotope labeling by amino acids in cell culture (SILAC) to selectively detect the incorporation of deuterium-labeled phenylalanine, tyrosine, and methionine into proteins in intact, single HeLa cells. The C−D stret

  5. Analytical system for stable carbon isotope measurements of low molecular weight (C2-C6) hydrocarbons

    NARCIS (Netherlands)

    Zuiderweg, A.T.; Holzinger, R.; Roeckmann, T.

    2011-01-01

    We present setup, testing and initial results from a new automated system for stable carbon isotope ratio measurements on C2 to C6 atmospheric hydrocarbons. The inlet system allows analysis of trace gases from air samples ranging from a few liters for urban samples and samples with high mixing ratio

  6. Electromagnetic separation of stable isotopes at the Institute of Atomic Energy, Academia Sinica

    Science.gov (United States)

    Ming-da, Hua; Gong-pan, Li; Shi-jun, Su; Nai-feng, Mao; Hung-yung, Lu

    1981-07-01

    For almost 20 years the Institute of Atomic Energy, Academia Sinica has been separating stable isotopes of the elements by electromagnetic separators and supplying these materials to research work in many fields of our country. In this article we shall attempt to outline the growth of the effort and describe the present situation.

  7. Technical Note: Calcium and carbon stable isotope ratios as paleodietary indicators.

    Science.gov (United States)

    Melin, Amanda D; Crowley, Brooke E; Brown, Shaun T; Wheatley, Patrick V; Moritz, Gillian L; Yit Yu, Fred Tuh; Bernard, Henry; DePaolo, Donald J; Jacobson, Andrew D; Dominy, Nathaniel J

    2014-08-01

    Calcium stable isotope ratios are hypothesized to vary as a function of trophic level. This premise raises the possibility of using calcium stable isotope ratios to study the dietary behaviors of fossil taxa and to test competing hypotheses on the adaptive origins of euprimates. To explore this concept, we measured the stable isotope composition of contemporary mammals in northern Borneo and northwestern Costa Rica, two communities with functional or phylogenetic relevance to primate origins. We found that bone collagen δ(13) C and δ(15) N values could differentiate trophic levels in each assemblage, a result that justifies the use of these systems to test the predicted inverse relationship between bioapatite δ(13) C and δ(44) Ca values. As expected, taxonomic carnivores (felids) showed a combination of high δ(13) C and low δ(44) Ca values; however, the δ(44) Ca values of other faunivores were indistinguishable from those of primary consumers. We suggest that the trophic insensitivity of most bioapatite δ(44) Ca values is attributable to the negligible calcium content of arthropod prey. Although the present results are inconclusive, the tandem analysis of δ(44) Ca and δ(13) C values in fossils continues to hold promise for informing paleodietary studies and we highlight this potential by drawing attention to the stable isotope composition of the Early Eocene primate Cantius.

  8. Verification of Egg Farming Systems from the Netherlands and New Zealand Using Stable Isotopes

    NARCIS (Netherlands)

    Rogers, Karyne M.; Ruth, Van Saskia; Alewijn, Martin; Philips, Andy; Rogers, Pam

    2015-01-01

    Stable isotopes were used to develop authentication criteria of eggs laid under cage, barn, free range, and organic farming regimens from The Netherlands and New Zealand. A training set of commercial poultry feeds and egg albumen from 49 poultry farms across The Netherlands was used to determine

  9. Diet of spotted bats (Euderma maculatum) in Arizona as indicated by fecal analysis and stable isotopes

    Science.gov (United States)

    We assessed diet of spotted bats (Euderma maculatum (J.A. Allen, 1891)) by visual analysis of bat feces and stable carbon (δ13C) and nitrogen (δ15N) isotope analysis of bat feces, wing, hair, and insect prey. We collected 33 fecal samples from spotted bats and trapped 3755 insect...

  10. Studies on accuracy of trichothecene multitoxin analysis using stable isotope dilution assays.

    Science.gov (United States)

    Asam, S; Rychlik, M

    2007-12-01

    Critical parameters in mycotoxin analysis were examined by using stable isotope-labelled tricho-thecenes. Sample weight was downsized to 1 g without loosing precision when sufficiently homogenized samples were taken for analysis. Complete extraction of trichothecenes could be achieved with a solvent mixture of acetonitrile+water (84+16; v+v) even without the use of stable isotope labelled standards. However, in particular for the analysis of deoxynivalenol the absolute amount of water in the solvent volume used for extraction appeared critical. Depending on the matrix a low water amount resulted in too low quantitative values when no stable isotope-labelled standards are applied to correct for incomplete extraction. In this case the used extraction volume had to be at least 10 ml for 1 g sample when acetonitrile + water (84+16; v+v) was used as extraction solvent.Losses during sample preparation using two different clean-up columns were not observed. On the contrary, matrix suppression in the ESI-interface of the LC-MS equipment was found to be a serious problem. Depending on the matrix, the latter effect resulted in considerably lower values for trichothecenes when no stable isotope-labelled standards were used to counterbalance this suppression.

  11. Coral cavity sponges depend on reef-derived food resources: stable isotope and fatty acid constraints

    NARCIS (Netherlands)

    Duyl, F.C. van; Moodley, L.; Nieuwland, G.; IJzerloo, L. van; Soest, R.W.M. van; Houtekamer, M.; Meesters, E.H.; Middelburg, J.J.

    2011-01-01

    The diet of cavity sponges on the narrow fringing reefs of Curac¸ao, Caribbean was studied. The origin and resources of the bulk food of these sponges, i.e., dissolved organic matter (DOM), were identified using stable carbon and nitrogen isotopes and fatty acid biomarkers. We found that

  12. Coral cavity sponges depend on reef-derived food resources: stable isotope and fatty acid constraints

    NARCIS (Netherlands)

    van Duyl, F.C.; Moodley, L.; Nieuwland, G.; van Ijzerloo, L.; van Soest, R.W.M.; Houtekamer, M.; Meesters, E.H.; Middelburg, J.J.

    2011-01-01

    The diet of cavity sponges on the narrow fringing reefs of Cura double dagger ao, Caribbean was studied. The origin and resources of the bulk food of these sponges, i.e., dissolved organic matter (DOM), were identified using stable carbon and nitrogen isotopes and fatty acid biomarkers. We found

  13. Coral cavity sponges depend on reef-derived food resources: stable isotope and fatty acid constraints

    NARCIS (Netherlands)

    van Duyl, F.C.; Moodley, L.; Nieuwland, G.; van IJzerloo, L.; van Soest, R.W.M.; Houtekamer, M.; Meesters, E.H.; Middelburg, J.J.

    2011-01-01

    The diet of cavity sponges on the narrow fringing reefs of Curaçao, Caribbean was studied. The origin and resources of the bulk food of these sponges, i.e., dissolved organic matter (DOM), were identified using stable carbon and nitrogen isotopes and fatty acid biomarkers. We found that

  14. CORRESPONDENCE OF STABLE ISOTOPE AND GUT CONTENTS ANALYSES IN DETERMINING TROPHIC POSITION OF STREAM FISHES

    Science.gov (United States)

    It is generally accepted that both stable isotope analysis (SIA) and gut contents analysis (GCA) be used in food web studies; however, few researchers have analyzed these data in concert. We utilized SIA and GCA to determine if longitudinal and seasonal variation in diet affects...

  15. Comparing trophic position of stream fishes using stable isotope and gut contents analyses

    Science.gov (United States)

    Stable isotope analysis (SIA) and gut content analysis (GCA) are commonly used in food web studies, but few studies analyze these data in concert. We used SIA and GCA to identify diets and trophic position (TP) of six stream fishes and to compare TP estimates between methods. Ord...

  16. ANALYTICAL EMPLOYMENT OF STABLE ISOTOPES OF CARBON, NITROGEN, OXYGEN AND HYDROGEN FOR FOOD AUTHENTICATION

    Directory of Open Access Journals (Sweden)

    E. Novelli

    2011-04-01

    Full Text Available Stable isotopes of carbon, nitrogen, oxygen and hydrogen were used for analytical purposes for the discrimination of the type of production (farming vs. fishing in the case of sea bass and for geographical origin in the case of milk. These results corroborate similar experimental evidences and confirm the potential of this analytical tool to support of food traceability.

  17. Noninvasive imaging of protein metabolic labeling in single human cells using stable isotopes and Raman microscopy

    NARCIS (Netherlands)

    van Manen, H.J.; Lenferink, Aufrid T.M.; Otto, Cornelis

    2008-01-01

    We have combined nonresonant Raman microspectroscopy and spectral imaging with stable isotope labeling by amino acids in cell culture (SILAC) to selectively detect the incorporation of deuterium-labeled phenylalanine, tyrosine, and methionine into proteins in intact, single HeLa cells. The C−D

  18. Stable carbon isotope fractionation of organic cyst-forming dinoflagellates: Evaluating the potential for a CO

    NARCIS (Netherlands)

    Hoins, M.; Van de Waal, D.B.; Eberlein, T.; Reichart, G.-J.; Rost, B.; Sluijs, A.

    2015-01-01

    Over the past decades, significant progress has been made regarding the quantification and mechanistic understanding of stable carbon isotope fractionation (13C fractionation) in photosynthetic unicellular organisms in response to changes in the partial pressure of atmospheric CO2 (pCO2). However, h

  19. Tracing the origins, migrations, and other movements of fishes using stable isotopes

    Science.gov (United States)

    Although stable isotope studies are increasingly used to trace fish origin, migration, and movement, the approach has not been as widely adopted as extrinsic marking and tagging. This is, at least in part, because many fishery scientists do not have a background in biogeochemistr...

  20. Stable isotope-mass spectrometric determination of semen transfer in malaria mosquitoes

    NARCIS (Netherlands)

    Helinski, M.E.H.; Hood-Nowotny, R.C.; Mayr, L.; Knols, B.G.J.

    2007-01-01

    The potential use of stable isotopes to study mosquito mating was investigated by tracing the fate of labelled semen into spermathecae. [13C]glucose was incorporated in the diet of the malaria mosquito Anopheles arabiensis. Treatments included labelling of either the larval water or adult sugar

  1. Analysis of stable isotopes in fish mucus during a controlled diet switch

    Science.gov (United States)

    We have used a controlled diet switch in steelhead trout (Oncorhynchus mykiss) at the Oregon Hatchery Research Center to study the time rates of changes in stable isotopes of carbon and nitrogen (13C and 15N) in epidermal mucus, a rapidly responding “tissue.” Because of the ra...

  2. Stable isotope analysis of fish mucus during a controlled diet switch

    Science.gov (United States)

    We have used a controlled diet switch in steelhead trout (Oncorhynchus mykiss) at the Oregon Hatchery Research Center to study the time rates of changes in stable isotopes of carbon and nitrogen (13C and 15N) in epidermal mucus, a rapidly responding “tissue.” Because of the ra...

  3. New Organic Stable Isotope Reference Materials for Distribution through the USGS and the IAEA

    Science.gov (United States)

    Schimmelmann, Arndt; Qi, Haiping

    2014-05-01

    The widespread adoption of relative stable isotope-ratio measurements in organic matter by diverse scientific disciplines is at odds with the dearth of international organic stable isotopic reference materials (RMs). Only two of the few carbon (C) and nitrogen (N) organic RMs, namely L-glutamic acids USGS40 and USGS41 [1], both available from the U.S. Geological Survey (USGS) and the International Atomic Energy Agency (IAEA), provide an isotopically contrasting pair of organic RMs to enable essential 2-point calibrations for δ-scale normalization [2, 3]. The supply of hydrogen (H) organic RMs is even more limited. Numerous stable isotope laboratories have resorted to questionable practices, for example by using 'CO2, N2, and H2 reference gas pulses' for isotopic calibrations, which violates the principle of identical treatment of sample and standard (i.e., organic unknowns should be calibrated directly against chemically similar organic RMs) [4], or by using only 1 anchor instead of 2 for scale calibration. The absence of international organic RMs frequently serves as an excuse for indefensible calibrations. In 2011, the U.S. National Science Foundation (NSF) funded an initiative of 10 laboratories from 7 countries to jointly develop much needed new organic RMs for future distribution by the USGS and the IAEA. The selection of targeted RMs attempts to cover various common compound classes of broad technical and scientific interest. We had to accept compromises to approach the ideal of high chemical stability, lack of toxicity, and low price of raw materials. Hazardous gases and flammable liquids were avoided in order to facilitate international shipping of future RMs. With the exception of polyethylene and vacuum pump oil, all organic RMs are individual, chemically-pure substances, which can be used for compound-specific isotopic measurements in conjunction with liquid and gas chromatographic interfaces. The compounds listed below are under isotopic calibration by

  4. Technical Note: Silica stable isotopes and silicification in a carnivorous sponge \\textit{Asbestopluma} sp.

    Directory of Open Access Journals (Sweden)

    K. R. Hendry

    2014-12-01

    Full Text Available The stable isotope composition of benthic sponge spicule silica is a potential source of palaeoceanographic information about past deep seawater chemistry. The silicon isotopic composition of spicules has been shown to relate to the silicic acid concentration of ambient water, although existing calibrations do exhibit a degree of scatter in the relationship. Less is known about how the oxygen isotope composition of sponge spicule silica relates to environmental conditions during growth. Here, we investigate the biological vital effects on silica silicon and oxygen isotope composition in a carnivorous sponge, Asbestopluma sp., from the Southern Ocean. We find significant variations in silicon and oxygen isotopic composition within the specimen that appear related to unusual spicule silicification. The largest variation in both isotope systems was associated to the differential distribution of an unconventional, hypersilicified spicule type (desma along the sponge body. The absence of an internal canal in the desmas suggests an unconventional silicification pattern leading to an unusually heavy isotopic signature. Additional internal variability derives from a systematic offset between the peripheral skeleton of the body having systematically a higher isotopic composition than the internal skeleton. A simplified silicon isotope fractionation model, in which desmas were excluded, suggests that the lack of a system for seawater pumping in carnivorous sponges favours a low replenishment of dissolved silicon within the internal tissues, causing kinetic fractionation during silicification that impacts the isotopic signature of the internal skeleton. Analysis of multiple spicules should be carried out to "average out" any artefacts in order to produce more robust downcore measurements.

  5. Stable Isotope Constraints on the Ocean from Hydrothermally-altered Igneous Rocks

    Science.gov (United States)

    Gregory, R. T.

    2007-12-01

    The 18O/16O ratio of the ocean provides an important constraint on the global geochemical cycles in the Precambrian Earth. The oxygen isotope ratio of the ocean is most likely buffered near its present day value as long as plate tectonics is operative. A quasi-steady state value for oxygen isotopes is reached on a 100 Myr timescale after the onset of plate tectonics. Hydrothermally-altered igneous rocks constrain the oxygen and hydrogen isotope value of the hydrosphere back through time. Whereas, the oxygen isotope composition of seawater owes its value to the competition between low temperature chemical weathering and mid-ocean ridge hydrothermal exchange, there is no such process for hydrogen isotopes. Changes in the oxygen isotope ratio of seawater should be reflected in hydrothermally altered rocks by the presence of low or high 18O exchanged igneous rocks with normal δD values. The distribution of D and 18O in hydrothermally rocks is used to infer the position of the meteoric water line back through time. Results from the Phanerozoic, the Proterozoic, and the Archean fail to confirm the hypothesis that the global oceans were ever strongly 18O-depleted. The meteoric water line is anchored to the isotopic composition of seawater, the isotope standard for both oxygen and hydrogen isotopes. The ability to use sedimentary rocks or other proxies for climate depend upon the variation in the stable isotopic composition of seawater. Thus far, the hydrothermal record does not support the existence of low 18O oceans. This suggests that low 18O values observed in carbonates and cherts result from either precipitation from oceans with higher temperature or from bodies of water isolated from the open ocean.

  6. Stable isotope anatomy of tropical cyclone Ita, North-Eastern Australia, April 2014.

    Science.gov (United States)

    Munksgaard, Niels C; Zwart, Costijn; Kurita, Naoyuki; Bass, Adrian; Nott, Jon; Bird, Michael I

    2015-01-01

    The isotope signatures registered in speleothems during tropical cyclones (TC) provides information about the frequency and intensity of past TCs but the precise relationship between isotopic composition and the meteorology of TCs remain uncertain. Here we present continuous δ18O and δ2H data in rainfall and water vapour, as well as in discrete rainfall samples, during the passage of TC Ita and relate the evolution in isotopic compositions to local and synoptic scale meteorological observations. High-resolution data revealed a close relationship between isotopic compositions and cyclonic features such as spiral rainbands, periods of stratiform rainfall and the arrival of subtropical and tropical air masses with changing oceanic and continental moisture sources. The isotopic compositions in discrete rainfall samples were remarkably constant along the ~450 km overland path of the cyclone when taking into account the direction and distance to the eye of the cyclone at each sampling time. Near simultaneous variations in δ18O and δ2H values in rainfall and vapour and a near-equilibrium rainfall-vapour isotope fractionation indicates strong isotopic exchange between rainfall and surface inflow of vapour during the approach of the cyclone. In contrast, after the passage of spiral rainbands close to the eye of the cyclone, different moisture sources for rainfall and vapour are reflected in diverging d-excess values. High-resolution isotope studies of modern TCs refine the interpretation of stable isotope signatures found in speleothems and other paleo archives and should aim to further investigate the influence of cyclone intensity and longevity on the isotopic composition of associated rainfall.

  7. Data-driven Approaches to Teaching Stable Isotopes in Hydrology and Environmental Geochemistry

    Science.gov (United States)

    Jefferson, A.; Merchant, W. R.; Dees, D.; Griffith, E. M.; Ortiz, J. D.

    2016-12-01

    Stable isotopes have revolutionized our understanding of watershed hydrology and other earth science processes. However, students may struggle to correctly interpret isotope ratios and few students understand how isotope measurements are made. New laser-based technologies lower the barrier to entry for giving students hands on experience with isotope measurements and data analysis. We hypothesizedthat integrating such activities into the curriculum would increase student content knowledge, perceptions, and motivation to learn. This project assessed the impact that different pedagogical approaches have on student learning of stable isotope concepts in upper-division geoscience courses. An isotope hydrograph separation module was developed and taught for a Watershed Hydrology course, and a Rayleigh distillation activity was developed and deployed for Environmental Geochemistry and Sedimentology/Stratigraphy classes. Groups of students were exposed to this content via (1) a lecture-only format; (2) a paper-based data analysis activity; and (3) hands-on data collection, sometimes including spectrometer analysis. Pre- and post-tests measured gains in content knowledge while approaches to learning and motivational questionnaires instruments were used to identify the effects of the classroom environment on learning approaches and motivation. Focus group interviews were also conducted to verify the quantitative data. All instructional styles appear to be equally effective at increasing student content knowledge of stable isotopes in the geosciences, but future studies need to move beyond "exam question" style assessment of learning. Our results may reflect that hands-on experiences are not new to upper-level geosciences students, because this is the way that many classes are taught in the geosciences (labs, field trips). Thus, active learning approaches may not have had the impact they would with other groups. The "messiness" of hands-on activities and authentic research

  8. Anomalous fluoride concentration in groundwater - is it natural or pollution? A stable isotope approach.

    Science.gov (United States)

    Marimon, Maria Paula Casagrande; Knöller, Kay; Roisenberg, Ari

    2007-06-01

    Fluoride anomalies (up to 11 mg/l) have been detected in groundwater of the central region of Rio Grande do Sul State, Southern Brazil, in an area where fluorosis is endemic. Two hypotheses are investigated concerning the fluoride origin: lithochemical affiliation from regional rock or contamination by fertilisers application. These hypotheses are discussed based on the stable isotope data of water, nitrate, and sulphate, which indicates that the local precipitation is the main groundwater recharge source. The isotopic composition of groundwater sulphate is similar to that of fertiliser sulphate. However, a conclusive assignment of groundwater sulphate to fertiliser origin is not indicated because further possible sulphate sources fall into the same isotopic range. In contrast, the isotopic composition of dissolved nitrate suggests that there is no direct relationship to the use of NPK fertilisers. Hence, an origin of the high fluoride content in groundwater related to long-term rock-water interactions seems likely.

  9. Tags for the stable isotopic labeling of carbohydrates and quantitative analysis by mass spectrometry.

    Science.gov (United States)

    Bowman, Michael J; Zaia, Joseph

    2007-08-01

    Although stable isotopic labeling has found widespread use in the proteomics field, its application to carbohydrate quantification has been limited. Herein we report the design, synthesis, and application of a novel series of compounds that allow for the incorporation of isotopic variation within glycan structures. The novel feature of the compounds is the ability to incorporate the isotopes in a controlled manner, allowing for the generation of four tags that vary only in their isotopic content. This allows for the direct comparisons of three samples or triplicate measurements with an internal standard within one mass spectral analysis. Quantitation of partially depolymerized glycosaminoglycan mixtures, as well as N-linked glycans released from fetuin, is used to demonstrate the utility of the tetraplex tagging strategy.

  10. Inferring foraging areas of nesting loggerhead turtles using satellite telemetry and stable isotopes.

    Directory of Open Access Journals (Sweden)

    Simona A Ceriani

    Full Text Available In recent years, the use of intrinsic markers such as stable isotopes to link breeding and foraging grounds of migratory species has increased. Nevertheless, several assumptions still must be tested to interpret isotopic patterns found in the marine realm. We used a combination of satellite telemetry and stable isotope analysis to (i identify key foraging grounds used by female loggerheads nesting in Florida and (ii examine the relationship between stable isotope ratios and post-nesting migration destinations. We collected tissue samples for stable isotope analysis from 14 females equipped with satellite tags and an additional 57 untracked nesting females. Telemetry identified three post-nesting migratory pathways and associated non-breeding foraging grounds: (1 a seasonal continental shelf-constrained migratory pattern along the northeast U.S. coastline, (2 a non-breeding residency in southern foraging areas and (3 a residency in the waters adjacent to the breeding area. Isotopic variability in both δ(13C and δ(15N among individuals allowed identification of three distinct foraging aggregations. We used discriminant function analysis to examine how well δ(13C and δ(15N predict female post-nesting migration destination. The discriminant analysis classified correctly the foraging ground used for all but one individual and was used to predict putative feeding areas of untracked turtles. We provide the first documentation that the continental shelf of the Mid- and South Atlantic Bights are prime foraging areas for a large number (61% of adult female loggerheads from the largest loggerhead nesting population in the western hemisphere and the second largest in the world. Our findings offer insights for future management efforts and suggest that this technique can be used to infer foraging strategies and residence areas in lieu of more expensive satellite telemetry, enabling sample sizes that are more representative at the population level.

  11. Integrating stomach content and stable isotope analyses to quantify the diets of pygoscelid penguins.

    Directory of Open Access Journals (Sweden)

    Michael J Polito

    Full Text Available Stomach content analysis (SCA and more recently stable isotope analysis (SIA integrated with isotopic mixing models have become common methods for dietary studies and provide insight into the foraging ecology of seabirds. However, both methods have drawbacks and biases that may result in difficulties in quantifying inter-annual and species-specific differences in diets. We used these two methods to simultaneously quantify the chick-rearing diet of Chinstrap (Pygoscelis antarctica and Gentoo (P. papua penguins and highlight methods of integrating SCA data to increase accuracy of diet composition estimates using SIA. SCA biomass estimates were highly variable and underestimated the importance of soft-bodied prey such as fish. Two-source, isotopic mixing model predictions were less variable and identified inter-annual and species-specific differences in the relative amounts of fish and krill in penguin diets not readily apparent using SCA. In contrast, multi-source isotopic mixing models had difficulty estimating the dietary contribution of fish species occupying similar trophic levels without refinement using SCA-derived otolith data. Overall, our ability to track inter-annual and species-specific differences in penguin diets using SIA was enhanced by integrating SCA data to isotopic mixing modes in three ways: 1 selecting appropriate prey sources, 2 weighting combinations of isotopically similar prey in two-source mixing models and 3 refining predicted contributions of isotopically similar prey in multi-source models.

  12. Microbial degradation of alpha-cypermethrin in soil by compound-specific stable isotope analysis

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Zemin [MOE Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058 (China); Shen, Xiaoli [MOE Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058 (China); Department of Environmental Engineering, Quzhou University, Quzhou 324000 (China); Zhang, Xi-Chang [Laboratory for Teaching in Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058 (China); Liu, Weiping [MOE Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058 (China); Yang, Fangxing, E-mail: fxyang@zju.edu.cn [MOE Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058 (China); Department of Effect-Directed Analysis, Helmholtz Center for Environmental Research – UFZ, Leipzig 04318 (Germany)

    2015-09-15

    Highlights: • Alpha-cypermethrin (α-CP) can be degraded by microorganisms in soil. • Biodegradation of α-CP resulted in carbon isotope fractionation. • A relationship was found between carbon isotope ratios and concentrations of α-CP. • An enrichment factor ϵ of α-CP was determined as −1.87‰. • CSIA is applicable to assess biodegradation of α-CP. - Abstract: To assess microbial degradation of alpha-cypermethrin in soil, attenuation of alpha-cypermethrin was investigated by compound-specific stable isotope analysis. The variations of the residual concentrations and stable carbon isotope ratios of alpha-cypermethrin were detected in unsterilized and sterilized soils spiked with alpha-cypermethrin. After an 80 days’ incubation, the concentrations of alpha-cypermethrin decreased to 0.47 and 3.41 mg/kg in the unsterilized soils spiked with 2 and 10 mg/kg, while those decreased to 1.43 and 6.61 mg/kg in the sterilized soils. Meanwhile, the carbon isotope ratios shifted to −29.14 ± 0.22‰ and −29.86 ± 0.33‰ in the unsterilized soils spiked with 2 and 10 mg/kg, respectively. The results revealed that microbial degradation contributed to the attenuation of alpha-cypermethrin and induced the carbon isotope fractionation. In order to quantitatively assess microbial degradation, a relationship between carbon isotope ratios and residual concentrations of alpha-cypermethrin was established according to Rayleigh equation. An enrichment factor, ϵ = −1.87‰ was obtained, which can be employed to assess microbial degradation of alpha-cypermethrin. The significant carbon isotope fractionation during microbial degradation suggests that CSIA is a proper approach to qualitatively detect and quantitatively assess the biodegradation during attenuation process of alpha-cypermethrin in the field.

  13. A direct measurement of the stable isotopes of transpired water vapor in a northern Michigan forest

    Science.gov (United States)

    Aron, P.; Poulsen, C. J.; Fiorella, R.

    2016-12-01

    The stable isotopes of oxygen and hydrogen in water vapor track hydrologic processes as phase changes of water preferentially partition heavy isotopes (18O and 2H) into the condensate and light isotopes (16O and 1H) into the vapor phase. As a result, the isotopic composition of water vapor can be used to identify water fluxes and cycling through natural environments. Forest water vapor is comprised of terrestrial (evaporation and transpiration) and atmospheric (tropospheric mixing, precipitation, and condensation) components. Within the isotopic record of forest water vapor, stable isotopes of transpired water (δT) comprise an important component but is typically either assumed to be non-fractionating or estimated indirectly. However, on small time scales (minutes to hours), non-steady state forest systems experience isotopic enrichment during early morning and late afternoon when transpiration rates are low. We deployed two Picarro Cavity Ring-Down spectrometers (L2120-i and L2130-i, respectively) in the University of Michigan Biological Station (UMBS) forest near Pellston, MI to measure the isotopic composition of near-surface ambient water vapor and the transpired vapor component directly. Both ambient and transpired water vapor were measured at three heights above the forest floor (2, 10, and 20 m) during August 2016. To measure species-specific water use, δT was measured on red maple (Acer rubrum) and northern red oak (Quercus rubra), two of the dominant tree types in the UMBS forest. This work represents the first direct measurement of δT in the UMBS forest and will help decouple local and species-specific hydrologic cycling. Beyond UMBS, this measurement will allow for a better understanding of species-specific plant hydraulics and help identify when the steady state approximation of transpiration is valid, which can be used to study water use and forest health.

  14. Long-term data set analysis of stable isotopic composition in German rivers

    Science.gov (United States)

    Reckerth, Anne; Stichler, Willibald; Schmidt, Axel; Stumpp, Christine

    2017-09-01

    Stable isotopes oxygen-18 (18O) and deuterium (2H) are commonly used to investigate hydrological processes in catchments. However, only a few isotope studies have been conducted on a large scale and rarely over long time periods. The objective of this study was to identify the spatial and seasonal variability of isotopic composition in river water and how it is affected by geographical and hydrological factors. The stable isotopic composition of river water has been measured in nine large river catchments in Germany for a time period of 12 years or 26 years. We conducted time series and correlation analyses to identify spatial and temporal patterns of the isotopic composition in the rivers. Further, we compared it to isotopic composition in local precipitation and catchments characteristics. In the majority of the rivers, the spatial and temporal patterns of precipitation were directly reflected in river water. The isotopic signals of the river water were time shifted and show attenuated amplitudes. Further deviations from isotopic compositions in local precipitation were observed in catchments with complex flow systems. These deviations were attributed to catchment processes and influences like evaporation, damming and storage. The seasonality of the isotopic composition was mainly determined by the discharge regimes of the rivers. We found correlations between isotopic long-term averages and catchment altitude as well as latitude and longitude, resulting in a northwest-southeast gradient. Furthermore, it was shown that long-term averages of d-excess were inversely related to flow length and catchment size, which indicates that evaporation enrichment has an impact on the isotopic composition even in catchments of humid climates. This study showed that isotopic composition in rivers can serve as a proxy for the local precipitation and can be utilized as an indicator for hydrological processes even in large river basins. In future, such long time series will help to

  15. Stable carbon isotope ratios of ambient secondary organic aerosols in Toronto

    Science.gov (United States)

    Saccon, M.; Kornilova, A.; Huang, L.; Moukhtar, S.; Rudolph, J.

    2015-09-01

    A method to quantify concentrations and stable carbon isotope ratios of secondary organic aerosols has been applied to study atmospheric nitrophenols in Toronto, Canada. The sampling of five nitrophenols, all with substantial secondary formation from the photooxidation of aromatic volatile organic compounds (VOCs), was conducted in the gas phase and particulate matter (PM) together and in PM alone. Their concentrations in the atmosphere are in the low ng m-3 range and, consequently, a large volume of air (> 1000 m3) is needed to analyze samples for stable carbon isotope ratios, resulting in sampling periods of typically 24 h. While this extended sampling period increases the representativeness of average values, it at the same time reduces possibilities to identify meteorological conditions or atmospheric pollution levels determining nitrophenol concentrations and isotope ratios. Average measured carbon isotope ratios of the different nitrophenols are between -34 and -33 ‰, which is well within the range predicted by mass balance. However, the observed carbon isotope ratios cover a range of nearly 9 ‰ and approximately 20 % of the isotope ratios of the products have isotope ratios lower than predicted from the kinetic isotope effect of the first step of the reaction mechanism and the isotope ratio of the precursor. This can be explained by isotope fractionation during reaction steps following the initial reaction of the precursor VOCs with the OH radical. Limited evidence for local production of nitrophenols is observed since sampling was done in the Toronto area, an urban center with significant anthropogenic emission sources. Strong evidence for significant local formation of nitrophenols is only found for samples collected in summer. On average, the difference in carbon isotope ratios between nitrophenols in the particle phase and in the gas phase is insignificant, but for a limited number of observations in summer, a substantial difference is observed. This

  16. Distribution and fractionation mechanism of stable carbon isotope of coalbed methane

    Institute of Scientific and Technical Information of China (English)

    QIN Shengfei; TANG Xiuyi; SONG Yan; WANG Hongyan

    2006-01-01

    The stable carbon isotope values of coalbed methane range widely,and also are generally lighter than that of gases in normal coal-formed gas fields with similar coal rank.There exists strong carbon isotope fractionation in coalbed methane and it makes the carbon isotope value lighter.The correlation between the carbon isotope value and Ro in coalbed methane is less obvious.The coaly source rock maturity cannot be judged by coalbed methane carbon isotope value.The carbon isotopes of coalbed methane become lighter in much different degree due to the hydrodynamics.The stronger the hydrodynamics is,the lighter the CBM carbon isotopic value becomes.Many previous investigations indicated that the desorption-diffusion effects make the carbon isotope value of coalbed methane lighter.However,the explanation has encountered many problems.The authors of this article suggest that the flowing groundwater dissolution to free methane in coal seams and the free methane exchange with absorbed one is the carbon isotope fractionation mechanism in coalbed methane.The flowing groundwater in coal can easily take more 13CH4 away from free gas and comparatively leave more 12CH4.This will make 12CH4 density in free gas comparatively higher than that in absorbed gas.The remaining 12CH4 in free gas then exchanges with the adsorbed methane in coal matrix.Some absorbed 13CH4 can be replaced and become free gas.Some free 12CH4 can be absorbed again into coal matrix and become absorbed gas.Part of the newly replaced 13CH4 in free gas will also be taken away by water,leaving preferentially more 12CH4.The remaining 12CH4 in free gas will exchange again with adsorbed methane in the coal matrix.These processes occur all the time.Through accumulative effect,the 12CH4 will be greatly concentrated in coal.Thus,the stable carbon isotope of coalbed methane becomes dramatically lighter.Through simulation experiment on water-dissolved methane,it had been proved that the flowing water could fractionate the

  17. Comparing compound-specific and bulk stable nitrogen isotope trophic discrimination factors across multiple freshwater fish species and diets

    Science.gov (United States)

    The use of nitrogen stable isotopes for estimation of animal trophic position has become an indispensable approach in food web ecology. Compound-specific isotope analysis of amino acids is a new approach for estimating trophic position that may overcome key issues associated with nitrogen stable iso...

  18. A multi-stable isotope framework to understand eutrophication in aquatic ecosystems.

    Science.gov (United States)

    Gooddy, Daren C; Lapworth, Dan J; Bennett, Sarah A; Heaton, Tim H E; Williams, Peter J; Surridge, Ben W J

    2016-01-01

    Eutrophication is a globally significant challenge facing aquatic ecosystems, associated with human induced enrichment of these ecosystems with nitrogen (N) and phosphorus (P). However, the limited availability of inherent labels for P and N has constrained understanding of the triggers for eutrophication in natural ecosystems and appropriate targeting of management responses. This paper proposes and evaluates a new multi-stable isotope framework that offers inherent labels to track biogeochemical reactions governing both P and N in natural ecosystems. The framework couples highly novel analysis of the oxygen isotope composition of phosphate (δ(18)OPO4) with dual isotope analysis of oxygen and N within nitrate (δ(15)NNO3, δ(18)ONO3) and with stable N isotope analysis in ammonium (δ(15)NNH4). The River Beult in England is used as an exemplar system for initial evaluation of this framework. Our data demonstrate the potential to use stable isotope labels to track the input and downstream fate of nutrients from point sources, on the basis of isotopic differentiation for both P and N between river water and waste water treatment work effluent (mean difference = +1.7‰ for δ(18)OPO4; +15.5‰ for δ(15)NNH4 (under high flow); +7.3‰ for δ(18)ONO3 and +4.4‰ for δ(15)NNO3). Stable isotope data reveal nutrient inputs to the river upstream of the waste water treatment works that are consistent with partially denitrified sewage or livestock sources of nitrate (δ(15)NNO3 range = +11.5 to +13.1‰) and with agricultural sources of phosphate (δ(18)OPO4 range = +16.6 to +19.0‰). The importance of abiotic and metabolic processes for the in-river fate of N and P are also explored through the stable isotope framework. Microbial uptake of ammonium to meet metabolic demand for N is suggested by substantial enrichment of δ(15)NNH4 (by 10.2‰ over a 100 m reach) under summer low flow conditions. Whilst the concentration of both nitrate and phosphate

  19. Pan-Arctic concentrations of mercury and stable isotope ratios of carbon (δ(13)C) and nitrogen (δ(15)N) in marine zooplankton.

    Science.gov (United States)

    Pomerleau, Corinne; Stern, Gary A; Pućko, Monika; Foster, Karen L; Macdonald, Robie W; Fortier, Louis

    2016-05-01

    Zooplankton play a central role in marine food webs, dictating the quantity and quality of energy available to upper trophic levels. They act as "keystone" species in transfer of mercury (Hg) up through the marine food chain. Here, we present the first Pan-Arctic overview of total and monomethylmercury concentrations (THg and MMHg) and stable isotope ratios of carbon (δ(13)C) and nitrogen (δ(15)N) in selected zooplankton species by assembling data collected between 1998 and 2012 from six arctic regions (Laptev Sea, Chukchi Sea, southeastern Beaufort Sea, Canadian Arctic Archipelago, Hudson Bay and northern Baffin Bay). MMHg concentrations in Calanus spp., Themisto spp. and Paraeuchaeta spp. were found to increase with higher δ(15)N and lower δ(13)C. The southern Beaufort Sea exhibited both the highest THg and MMHg concentrations. Biomagnification of MMHg between Calanus spp. and two of its known predators, Themisto spp. and Paraeuchaeta spp., was greatest in the southern Beaufort Sea. Our results show large geographical variations in Hg concentrations and isotopic signatures for individual species related to regional ecosystem features, such as varying water masses and freshwater inputs, and highlight the increased exposure to Hg in the marine food chain of the southern Beaufort Sea.

  20. Bird migration and avian influenza: a comparison of hydrogen stable isotopes and satellite tracking methods

    Science.gov (United States)

    Bridge, Eli S.; Kelly, Jeffrey F.; Xiao, Xiangming; Takekawa, John Y.; Hill, Nichola J.; Yamage, Mat; Haque, Enam Ul; Islam, Mohammad Anwarul; Mundkur, Taej; Yavuz, Kiraz Erciyas; Leader, Paul; Leung, Connie Y.H.; Smith, Bena; Spragens, Kyle A.; Vandegrift, Kurt J.; Hosseini, Parviez R.; Saif, Samia; Mohsanin, Samiul; Mikolon, Andrea; Islam, Ausrafal; George, Acty; Sivananinthaperumal, Balachandran; Daszak, Peter; Newman, Scott H.

    2014-01-01

    Satellite-based tracking of migratory waterfowl is an important tool for understanding the potential role of wild birds in the long-distance transmission of highly pathogenic avian influenza. However, employing this technique on a continental scale is prohibitively expensive. This study explores the utility of stable isotope ratios in feathers in examining both the distances traveled by migratory birds and variation in migration behavior. We compared the satellite-derived movement data of 22 ducks from 8 species captured at wintering areas in Bangladesh, Turkey, and Hong Kong with deuterium ratios (δD) in the feathers of these and other individuals captured at the same locations. We derived likely molting locations from the satellite tracking data and generated expected isotope ratios based on an interpolated map of δD in rainwater. Although δD was correlated with the distance between wintering and molting locations, surprisingly, measured δD values were not correlated with either expected values or latitudes of molting sites. However, population-level parameters derived from the satellite-tracking data, such as mean distance between wintering and molting locations and variation in migration distance, were reflected by means and variation of the stable isotope values. Our findings call into question the relevance of the rainfall isotope map for Asia for linking feather isotopes to molting locations, and underscore the need for extensive ground truthing in the form of feather-based isoscapes. Nevertheless, stable isotopes from feathers could inform disease models by characterizing the degree to which regional breeding populations interact at common wintering locations. Feather isotopes also could aid in surveying wintering locations to determine where high-resolution tracking techniques (e.g. satellite tracking) could most effectively be employed. Moreover, intrinsic markers such as stable isotopes offer the only means of inferring movement information from

  1. Quinone-based stable isotope probing for assessment of 13C substrate-utilizing bacteria

    Science.gov (United States)

    Kunihiro, Tadao; Katayama, Arata; Demachi, Toyoko; Veuger, Bart; Boschker, Henricus T. S.; van Oevelen, Dick

    2015-04-01

    In this study, we attempted to establish quinone-stable-isotope probing (SIP) technique to link substrate-utilizing bacterial group to chemotaxonomic group in bacterial community. To identify metabolically active bacterial group in various environments, SIP techniques combined with biomarkers have been widely utilized as an attractive method for environmental study. Quantitative approaches of the SIP technique have unique advantage to assess substrate-incorporation into bacteria. As a most major quantitative approach, SIP technique based on phospholipid-derived fatty acids (PLFA) have been applied to simultaneously assess substrate-incorporation rate into bacteria and microbial community structure. This approach is powerful to estimate the incorporation rate because of the high sensitivity due to the detection by a gas chromatograph-combustion interface-isotope ratio mass spectrometer (GC-c-IRMS). However, its phylogenetic resolution is limited by specificity of a compound-specific marker. We focused on respiratory quinone as a biomarker. Our previous study found a good correlation between concentrations of bacteria-specific PLFAs and quinones over several orders of magnitude in various marine sediments, and the quinone method has a higher resolution (bacterial phylum level) for resolving differences in bacterial community composition more than that of bacterial PLFA. Therefore, respiratory quinones are potentially good biomarkers for quantitative approaches of the SIP technique. The LC-APCI-MS method as molecular-mass based detection method for quinone was developed and provides useful structural information for identifying quinone molecular species in environmental samples. LC-MS/MS on hybrid triple quadrupole/linear ion trap, which enables to simultaneously identify and quantify compounds in a single analysis, can detect high molecular compounds with their isotope ions. Use of LC-MS/MS allows us to develop quinone-SIP based on molecular mass differences due to

  2. Late Glacial Tropical Savannas in Sundaland Inferred From Stable Carbon Isotope Records of Cave Guano

    Science.gov (United States)

    Wurster, C. M.; Bird, M. I.; Bull, I.; Dungait, J.; Bryant, C. L.; Ertunç, T.; Hunt, C.; Lewis, H. A.; Paz, V.

    2008-12-01

    During the Last Glacial Period (LGP), reduced global sea level exposed the continental shelf south of Thailand to Sumatra, Java, and Borneo to form the contiguous continent of Sundaland. However, the type and extent of vegetation that existed on much of this exposed landmass during the LGP remains speculative. Extensive bird and bat guano deposits in caves throughout this region span beyond 40,000 yr BP, and contain a wealth of untapped stratigraphic palaeoenvironmental information. Stable carbon isotope ratios of insectivorous bird and bat guano contain a reliable record of the animal's diet and, through non-specific insect predation, reflect the relative abundance of major physiological pathways in plants. Various physiological pathways of carbon fixation in plants yield differing stable carbon isotope ratios. Stable carbon isotope values of C3 plants are lower than C4 vegetation due to different enzymatic discriminations of the heavy isotope through the carbon fixing pathways. In tropical locales, grasses nearly always follow the C4 photosynthetic pathway, whereas tropical rainforest uses C3 photosynthesis, providing a proxy for vegetation and therefore climate change in the past. Here we discuss four guano stable-isotope records, based on insect cuticle and n-alkane analysis, supplemented by pollen analysis. All sites suggest a C3 dominated ecosystem for the Holocene, consistent with the wet tropical forest vegetation present at all locations. Two sites from Palawan Island, Philippines, record stable carbon isotope values of guano that document a drastic change from C3 (forest) to C4 (savanna) dominated ecosystems during the Last Glacial Maximum (LGM). A third location, at Niah Great Cave, Malaysia, indicates C3-dominant vegetation throughout the record, but does display variation in stable carbon isotope values likely linked to humidity changes. A fourth location, Batu Caves in Peninsular Malaysia, also indicates open vegetation during the LGM. Vegetation

  3. Integration of Stable Isotope and other Mass Spectral Data for Microbial Forensics

    Science.gov (United States)

    Kreuzer-Martin, H. W.; Jarman, K. H.

    2008-12-01

    The nascent field of microbial forensics requires the development of diverse signatures as indicators of various aspects of the production environment of microorganisms. We have characterized isotopic relationships between Bacillus subtilis ATCC 6051 spores and their growth environment, using as a database the carbon, nitrogen, oxygen and hydrogen stable isotope ratios of a total of 247 separate cultures of spores produced on a total of 32 different culture media. We have analyzed variation within individual samples, between cultures produced in tandem, and between cultures produced in the same medium but at different times in the context of using stable isotope ratios as a signature for sample matching. We have correlated the stable isotope ratios of carbon, nitrogen, oxygen, and hydrogen of growth medium nutrients or water and spores and show examples of how these relationships can be used to exclude nutrient or water samples as possible growth substrates for specific cultures. The power of stable isotope ratio data can be greatly enhanced by combining it with orthogonal datasets that speak to different aspects of an organism's production environment. We developed a Bayesian network that follows the causal relationship from culture medium recipe to spore elemental content as measured by secondary ion mass spectrometry (SIMS), carbon and nitrogen stable isotope ratios, and to the presence of residual agar by electrospray ionization MS (ESI-MS). The network was developed and tested on data from three replicate cultures of B. subtilis ATCC 49760 in broth and agar-containing versions of four different nutrient media. To test the network, data from SIMS analyses of B. subtilis 49760 produced in a different medium, from approximately 200 ESI MS analyses of B. thuringensis ATCC 58890 and B. anthracis Sterne grown in five additional media, and the stable isotope data from the 247 cultures of B. subtilis 6051 spores were used. This network was able to characterize

  4. Assessing connectivity of estuarine fishes based on stable isotope ratio analysis

    Science.gov (United States)

    Herzka, Sharon Z.

    2005-07-01

    Assessing connectivity is fundamental to understanding the population dynamics of fishes. I propose that isotopic analyses can greatly contribute to studies of connectivity in estuarine fishes due to the high diversity of isotopic signatures found among estuarine habitats and the fact that variations in isotopic composition at the base of a food web are reflected in the tissues of consumers. Isotopic analysis can be used for identifying nursery habitats and estimating their contribution to adult populations. If movement to a new habitat is accompanied by a shift to foods of distinct isotopic composition, recent immigrants and residents can be distinguished based on their isotopic ratios. Movement patterns thus can be reconstructed based on information obtained from individuals. A key consideration is the rate of isotopic turnover, which determines the length of time that an immigrant to a given habitat will be distinguishable from a longtime resident. A literature survey indicated that few studies have measured turnover rates in fishes and that these have focused on larvae and juveniles. These studies reveal that biomass gain is the primary process driving turnover rates, while metabolic turnover is either minimal or undetectable. Using a simple dilution model and biomass-specific growth rates, I estimated that young fishes with fast growth rates will reflect the isotopic composition of a new diet within days or weeks. Older or slower-growing individuals may take years or never fully equilibrate. Future studies should evaluate the factors that influence turnover rates in fishes during various stages of the life cycle and in different tissues, as well as explore the potential for combining stable isotope and otolith microstructure analyses to examine the relationship between demographic parameters, movement and connectivity.

  5. Stable carbon and oxygen isotope study on benthic foraminifera: Implication for microhabitat preferences and interspecies correlation

    Indian Academy of Sciences (India)

    Ajoy K Bhaumik; Shiv Kumar; Shilpi Ray; G K Vishwakarma; Anil K Gupta; Pushpendra Kumar; Kalachand Sain

    2017-07-01

    Stable isotopes of benthic foraminifera have widely been applied in micropalaeontological research to understand vital effects in foraminifera. Isotopic fractionations are mainly controlled by ontogeny, bottom/pore water chemistry, habitat preference, kinetic effect and respiration. Discontinuous abundance of a species for isotopic analysis has forced us to select multiple species from down-core samples. Thus standardisation factors are required to convert isotopic values of one species with respect to other species. The present study is pursued on isotopic values of different pairs of benthic foraminifera from the Krishna–Godavari basin and Peru offshore to understand habitat-wise isotopic variation and estimation of isotopic correction factors for the paired species (Cibicides wuellerstorfi–Bulimina marginata, Ammonia spp.–Loxostomum amygdalaeformis and Bolivina seminuda–Nonionella auris). Infaunal species (B. marginata, Ammonia spp. and N. auris) show a lighter carbon isotopic excursion with respect to the epifaunal to shallow infaunal forms (C. wuellerstorfi, L. amygdalaeformis and B. seminuda). These lighter δ13C values are related to utilisation of CO2 produced by anaerobic remineralisation of organic matter. However, enrichment of δ18O for the deeper microhabitat (bearing lower pH and decreased CO32−) is only recorded in case of B. marginata. It is reverse in case of N. auris and related to utilisation of respiratory CO2 and internal dissolve inorganic carbon pool. Estimation of interspecies isotopic correction factors for the species pairs (δ13C of C. wuellerstorfi–B. marginata, L. amygdalaeformis–Ammonia spp., N. auris–B. seminuda) and δ18O of C. wuellerstorfi–B. marginata are statistically reliable and may be used in palaeoecological studies.

  6. Spatial variations in food web structures with alternative stable states: evidence from stable isotope analysis in a large eutrophic lake

    Science.gov (United States)

    Li, Yunkai; Zhang, Yuying; Xu, Jun; Zhang, Shuo

    2017-05-01

    Food web structures are well known to vary widely among ecosystems. Moreover, many food web studies of lakes have generally attempted to characterize the overall food web structure and have largely ignored internal spatial and environmental variations. In this study, we hypothesize that there is a high degree of spatial heterogeneity within an ecosystem and such heterogeneity may lead to strong variations in environmental conditions and resource availability, in turn resulting in different trophic pathways. Stable carbon and nitrogen isotopes were employed for the whole food web to describe the structure of the food web in different sub-basins within Taihu Lake. This lake is a large eutrophic freshwater lake that has been intensively managed and highly influenced by human activities for more than 50 years. The results show significant isotopic differences between basins with different environmental characteristics. Such differences likely result from isotopic baseline differences combining with a shift in food web structure. Both are related to local spatial heterogeneity in nutrient loading in waters. Such variation should be explicitly considered in future food web studies and ecosystem-based management in this lake ecosystem.

  7. Noninvasive imaging of protein metabolic labeling in single human cells using stable isotopes and Raman microscopy.

    Science.gov (United States)

    van Manen, Henk-Jan; Lenferink, Aufried; Otto, Cees

    2008-12-15

    We have combined nonresonant Raman microspectroscopy and spectral imaging with stable isotope labeling by amino acids in cell culture (SILAC) to selectively detect the incorporation of deuterium-labeled phenylalanine, tyrosine, and methionine into proteins in intact, single HeLa cells. The C-D stretching vibrational bands in these amino acids are observed in the 2100-2300 cm(-1) spectral region that is devoid of vibrational contributions from other, nondeuterated intracellular constituents. We found that incubation with deuterated amino acids for 8 h in cell culture already led to clearly detectable isotope-related signals in Raman spectra of HeLa cells. As expected, the level of isotope incorporation into proteins increased with incubation time, reaching 55% for deuterated phenylalanine after 28 h. Raman spectral imaging of HeLa cells incubated with deuterium-labeled amino acids showed similar spatial distributions for both isotope-labeled and unlabeled proteins, as evidenced by Raman ratio imaging. The SILAC-Raman methodology presented here combines the strengths of stable isotopic labeling of cells with the nondestructive and quantitative nature of Raman chemical imaging and is likely to become a powerful tool in both cell biology applications and research on tissues or whole organisms.

  8. Stable sulfur and oxygen isotopes as geochemical tracers of sulfate in karst waters

    Science.gov (United States)

    Sun, Jing; Kobayashi, Tatsuaki; Strosnider, William H. J.; Wu, Pan

    2017-08-01

    Karst water resources, which are extremely sensitive to mining activities, are critical for the support of human societies and ecological systems in many regions worldwide. In order to determine the sources and fate of dissolved sulfate in low-pH karst waters, hydrochemical variations of karst waters with and without acid mine drainage (AMD) impacts were investigated along with stable isotope dynamics. As expected, hydrochemical characteristics and isotopic compositions of the AMD and AMD-downstream water (ADW) were dramatically different from that of the non-AMD-impacted water (NAW). The sources of sulfur isotopes in sulfate were predominantly pyrite oxidation for the AMD and ADW, and atmospheric deposition for the NAW. Based on the general isotope-balance model, the relative proportions of sulfate oxygen derived from water and air were calculated. The mean proportion of sulfate oxygen derived from water in ADW was roughly double that of AMD. This suggests that the sulfate associated with AMD is predominantly influenced by aerobic pyrite oxidation, while that of ADW is likely affected by the dissolution of pyrite under anaerobic conditions in reservoir sediment. This observation was coincident with the noted variations of hydrochemical characteristics and was supported by principal component analysis. These results provide a better understanding of how stable isotopes of sulfate and water can be used to track mining contamination in karst aquifers, which could benefit remediation planning for these distinctive systems.

  9. Non-traditional stable isotope behaviors in immiscible silica-melts in a mafic magma chamber.

    Science.gov (United States)

    Zhu, Dan; Bao, Huiming; Liu, Yun

    2015-12-01

    Non-traditional stable isotopes have increasingly been applied to studies of igneous processes including planetary differentiation. Equilibrium isotope fractionation of these elements in silicates is expected to be negligible at magmatic temperatures (δ(57)Fe difference often less than 0.2 per mil). However, an increasing number of data has revealed a puzzling observation, e.g., the δ(57)Fe for silicic magmas ranges from 0‰ up to 0.6‰, with the most positive δ(57)Fe almost exclusively found in A-type granitoids. Several interpretations have been proposed by different research groups, but these have so far failed to explain some aspects of the observations. Here we propose a dynamic, diffusion-induced isotope fractionation model that assumes Si-melts are growing and ascending immiscibly in a Fe-rich bulk magma chamber. Our model offers predictions on the behavior of non-traditional stable isotope such as Fe, Mg, Si, and Li that are consistent with observations from many A-type granitoids, especially those associated with layered intrusions. Diffusion-induced isotope fractionation may be more commonly preserved in magmatic rocks than was originally predicted.

  10. Direct lead isotope analysis in Hg-rich sulfides by LA-MC-ICP-MS with a gas exchange device and matrix-matched calibration.

    Science.gov (United States)

    Zhang, Wen; Hu, Zhaochu; Günther, Detlef; Liu, Yongsheng; Ling, Wenli; Zong, Keqing; Chen, Haihong; Gao, Shan

    2016-12-15

    In situ Pb isotope data of sulfide samples measured by LA-MC-ICP-MS provide valuable geochemical information for studies of the origin and evolution of ore deposits. However, the severe isobaric interference of (204)Hg on (204)Pb and the lack of matrix-matched sulfide reference materials limit the precision of Pb isotopic analyses for Hg-rich sulfides. In this study, we observe that Hg forms vapor and can be completely removed from sample aerosol particles produced by laser ablation using a gas exchange device. Additionally, this device does not influence the signal intensities of Pb isotopes. The within-run precision, the external reproducibility and the analytical accuracy are significantly improved for the Hg-rich sulfide samples using this mercury-vapor-removing device. Matrix effects are observed when using silicate glass reference materials as the external standards to assess the relationship of mass fractionation factors between Tl and Pb in sulfide samples, resulting in a maximum deviation of ∼0.20% for (20x)Pb/(204)Pb. Matrix-matched reference materials are therefore required for the highly precise and accurate Pb isotope analyses of sulfide samples. We investigated two sulfide samples, MASS-1 (the Unites States Geological Survey reference materials) and Sph-HYLM (a natural sphalerite), as potential candidates. Repeated analyses of the two proposed sulfide reference materials by LA-MC-ICP-MS yield good external reproducibility of ICP-MS, demonstrating the reliability and robustness of our analytical protocol. Copyright © 2016 Elsevier B.V. All rights reserved.

  11. Using stable isotopes of nitrogen and carbon to study seabird ecology: applications in the Mediterranean seabird community

    Directory of Open Access Journals (Sweden)

    Manuela G. Forero

    2003-07-01

    Full Text Available The application of the stable isotope technique to ecological studies is becoming increasingly widespread. In the case of seabirds, stable isotopes of nitrogen and carbon have been mainly used as dietary tracers. This approach relieson the fact that food web isotopic signatures are reflected in the tissues of the consumer. In addition to the study of trophic ecology, stable isotopes have been used to track the movement of seabirds across isotopic gradients, as individuals moving between isotopically distinct foodwebs can carry with them information on the location of previous feeding areas. Studies applying the stable isotope methodology to the study of seabird ecology show a clear evolution from broad and descriptive approaches to detailed and individual-based analyses. The purpose of this article is to show the different fields of application of stable isotopes to the study of the seabird ecology. Finally, we illustrate the utility of this technique by considering the particularities of the Mediterranean seabird community, suggesting different ecological questions and conservation problems that could be addressed by using the stable isotope approach in this community.

  12. Searching for the true diet of marine predators: incorporating Bayesian priors into stable isotope mixing models.

    Directory of Open Access Journals (Sweden)

    André Chiaradia

    Full Text Available Reconstructing the diet of top marine predators is of great significance in several key areas of applied ecology, requiring accurate estimation of their true diet. However, from conventional stomach content analysis to recent stable isotope and DNA analyses, no one method is bias or error free. Here, we evaluated the accuracy of recent methods to estimate the actual proportion of a controlled diet fed to a top-predator seabird, the Little penguin (Eudyptula minor. We combined published DNA data of penguins scats with blood plasma δ(15N and δ(13C values to reconstruct the diet of individual penguins fed experimentally. Mismatch between controlled (true ingested diet and dietary estimates obtained through the separately use of stable isotope and DNA data suggested some degree of differences in prey assimilation (stable isotope and digestion rates (DNA analysis. In contrast, combined posterior isotope mixing model with DNA Bayesian priors provided the closest match to the true diet. We provided the first evidence suggesting that the combined use of these complementary techniques may provide better estimates of the actual diet of top marine predators- a powerful tool in applied ecology in the search for the true consumed diet.

  13. Stable carbon isotopic composition of gasolines determined by isotope ratio monitoring gas chromatography mass spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Smallwood, B.J.; Philp, R.P.; Allen, J.D. [University of Oklahoma, Norman, OK (United States). School of Geology and Geophysics

    2002-07-01

    A large number of underground gasoline storage facilities in the United States continuously leak gasoline into the subsurface, which makes gasoline a major groundwater contaminant. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) are used currently to characterize contaminated groundwater and soils. Correlations of contaminants with suspected source(s) are extremely difficult by these techniques because many gasolines have similar hydrocarbon distributions. The present study applied the technique of isotope ratio monitoring gas chromatography-mass spectrometry (irmGC-MS) to 19 gasoline samples from different areas of the USA. This allows a much better correlation of gasoline contaminants to source. Data obtained indicate a wide range of {sup {delta}}{sup 13}C values for 16 ubiquitous compounds in the gasolines. The majority of samples could be distinguished from each other on the basis of {sup {delta}}{sup 13}C hydrocarbon composition. The oxygenated additive methyl tertiary butyl ether (MTBE) was present in ten of the gasolines analyzed, and had a relatively narrow range of {sup {delta}}{sup 13}C values (-30.4 to -28.3 per mille). Preliminary investigations were also made to determine the extent of carbon isotopic fractionation after simple water washing and evaporation experiments. Results indicate that the majority of compounds did not undergo significant carbon isotopic fractionation as a result of these processes. (author)

  14. Application of stable carbon isotopes in long term mesocosm studies for carbon cycle investigation

    Science.gov (United States)

    Esposito, Mario

    2016-04-01

    Carbon dioxide (CO2) is an effective greenhouse gas. The Oceans absorb ca. 30% of the anthropogenic CO2 emissions and thereby partly attenuate deleterious climate effects. A consequence of the oceanic CO2 uptake is a decreased seawater pH and planktonic community shifts. The quantification of the anthropogenic perturbation was investigated through stable carbon isotope analysis in three "long term" mesocosm experiments (Sweden 2013, Gran Canaria 2014, Norway 2015) which reproduced near natural ecosystem conditions under both controlled and modified future CO2 level (up to 2000 ppm) scenarios. Parallel measurements of the stable isotope composition of dissolved inorganic carbon (δ13CDIC) dissolved organic carbon (δ13CDOC) and particulate carbon (δ13CTPC) both from the mesocosms water column and sediment traps showed similar trends in all the three experiments. A CO2 response was noticeable in the isotopic dataset, but increased CO2 levels had only a subtle effect on the concentrations of the dissolved and particulate organic carbon pool. Distinctive δ13C signatures of the particulate carbon pool both in the water column and the sediments were detectable for the different CO2 treatments and they were strongly correlated with the δ13CDIC signatures but not with the δ13CDOC pool. The validity of the isotopic data was verified by cross-analyses of multiple substances of known isotopic signatures on a GasBench, Elemental Analyser (EA) and on an in-house TOC-IRMS setup for the analysis of δ13CDIC, δ13CTPC and δ13CDOC, respectively. Results from these mesocosm experiments proved the stable carbon isotope approach to be an effective tool for quantifying the uptake and carbon transfer among the various compartments of the marine carbon system.

  15. The Effect of Aerosol Formation on Stable Isotopes Ratio in Titan's Atmosphere

    Science.gov (United States)

    Gautier, Thomas; Trainer, Melissa G.; Sebree, Joshua; Wold, Allison; Stern, Jennifer

    2016-10-01

    The formation of large amounts of aerosol in Titan atmosphere induces a significant sink for carbon and nitrogen in the atmosphere. Due to the high complexity of the chemistry leading to aerosol formation, there may be isotopic fractionation along the formation pathways of the aerosol. So far several stable isotopes have been measured in Titan atmosphere including the 13C/12C, 15N/14N and D/H ratios for different gaseous species. However, the fractionation effect of the aerosol formation and its impact on atmospheric stable isotope ratios has yet to be fully understood. Two experimental studies were recently published on the stable carbon [1] and nitrogen [1,2] isotope fractionation during aerosol formation in N2-CH4 reactant mixture. To better constrain the fractionation effect of aerosol formation on the Titan atmosphere we have measured the isotopic fractionation induced in laboratory aerosol analogues produced exploring the space of parameters that are expected to have an effect on fractionation processes. Parameters studied include pressure and temperature of aerosol formation and the reactant gas phase composition, including the standard "Titan" mixture of CH4/N2 as well as other trace species such as benzene (C6H6).[1] Sebree, J.A., Stern, J.C., Mandt, K.E., Domagal-Goldman, S.D., and Trainer, M.G.: C and N Fractionation of CH /N Mixtures during Photochemical Aerosol Formation: Relevance to Titan, (2016) Icarus 270:421-428[2] Kuga, M., Carrasco, N., Marty, B., Marrochi, Y., Bernard, S., Rigaudier, T., Fleury, B., Tissandier, L.: Nitrogen isotopic fractionation during abiotic synthesis of organic solid particles, (2014) EPSL 393:2-13

  16. Isotopic equilibration between dissolved and suspended particulate lead in the Atlantic Ocean - Evidence from Pb-210 and stable Pb isotopes

    Science.gov (United States)

    Sherrell, Robert M.; Boyle, Edward A.; Hamelin, Bruno

    1992-01-01

    Vertical profiles of, on one hand, the stable Pb isotopic composition, and on the other, the ratio of total Pb to Pb-210 in suspended particles, are noted to closely track contemporaneous depth variations in these ratios for dissolved Pb throughout the upper 2 km of the Sargasso Sea near Bermuda. A simple flux model suggests that the effect of deep ocean scavenging processes on the flux and isotopic composition of Pb sinking on large particles was minor throughout the preanthropogenic, and most of the anthropogenic era: but it has become more important as surface inputs decrease to preleaded gasoline levels, perhaps exceeding the contribution of surface-derived Pb flux in the next decade.

  17. Experimental assessment of environmental influences on the stable isotopic composition of Daphnia pulicaria and their ephippia

    Science.gov (United States)

    Schilder, J.; Tellenbach, C.; Möst, M.; Spaak, P.; van Hardenbroek, M.; Wooller, M. J.; Heiri, O.

    2015-02-01

    The stable isotopic composition of fossil resting eggs (ephippia) of Daphnia spp. is being used to reconstruct past environmental conditions in lake ecosystems. However, the underlying assumption that the stable isotopic composition of the ephippia reflects the stable isotopic composition of the parent Daphnia, of their diet and of the environmental water have yet to be confirmed in a controlled experimental setting. We performed experiments with Daphnia pulicaria cultures, which included a control treatment conducted at 12 °C in filtered lake water and with a diet of fresh algae, and three treatments in which we manipulated the stable carbon isotopic composition (δ13C value) of the algae, stable oxygen isotopic composition (δ18O value) of the water, and the water temperature, respectively. The stable nitrogen isotopic composition (δ15N value) of the algae was similar for all treatments. At 12 °C, differences in algal δ13C values and in δ18O values of water are reflected in those of Daphnia. The differences between ephippia and Daphnia stable isotope ratios were similar in the different treatments (δ13C: + 0.2 ± 0.4‰ (SD); δ15N: -1.6 ± 0.4‰; δ18O: -0.9 ± 0.4‰) indicating that changes in dietary δ13C and δ18O values of water are passed on to these fossilizing structures. A higher water temperature (20 °C) resulted in lower δ13C values in Daphnia and ephippia than in the other treatments with the same food source and in a minor change in the difference between δ13C values of ephippia and Daphnia (to -1.3 ± 0.3‰). This may have been due to microbial processes or increased algal respiration rates in the experimental containers, which may not affect Daphnia in natural environments. There was no significant difference in the offset between δ18O and δ15N values of ephippia and Daphnia between the 12 °C and 20 °C treatments, but the δ18O values of Daphnia and ephippia were on average 1.2‰ lower at 20 °C compared with 12 °C. We conclude

  18. [Meta-analysis of stable carbon and nitrogen isotopic enrichment factors for aquatic animals].

    Science.gov (United States)

    Guo, Liang; Sun, Cui-ping; Ren, Wei-zheng; Zhang, Jian; Tang, Jian-iun; Hu, Liana-liang; Chen, Xin

    2016-02-01

    Isotopic enrichment factor (Δ, the difference between the δ value of food and a consumer tissue) is an important parameter in using stable isotope analysis (SIA) to reconstruct diets, characterize trophic relationships, elucidate patterns of resource allocation, and construct food webs. Isotopic enrichment factor has been considered as a constancy value across a broad range of animals. However, recent studies showed that the isotopic enrichment factor differed among various types of animals although the magnitude of variation was not clear. Here, we conducted a meta-analysis to synthesize and compare Δ13C and Δ15N among four types of aquatic animals (teleosts, crustaceans, reptiles and molluscs). We searched for papers published before 2014 on Web of Science and CNKI using the key words "stable isotope or isotopic fractionation or fractionation factor or isotopic enrichment or trophic enrichment". Forty-two publications that contain 140 studies on Δ13C and 159 studies on Δ15N were obtained. We conducted three parallel meta-analyses by using three types of weights (the reciprocal of variance as weights, the sample size as weights, and equal weights). The results showed that no significant difference in Δ13C among different animal types (teleosts 1.0 per thousand, crustaceans 1.3 per thousand, reptiles 0.5 per thousand, and molluscs 1.5 per thousand), while Δ15N values were significantly different (teleosts 2.4 per thousand, crustaceans 3.6 per thousand, reptiles 1.0 per thousand and molluscs 2.5 per thousand). Our results suggested that the overall mean of Δ13C could be used as a general enrichment factor, but Δ15N should be chosen according to the type of aquatic animals in using SIA to analyze trophic relationships, patterns of resource allocation and food webs.

  19. Using stable isotopes to assess carbon and nitrogen turnover in the Arctic sympagic amphipod Onisimus litoralis.

    Science.gov (United States)

    Kaufman, Mette R; Gradinger, Rolf R; Bluhm, Bodil A; O'Brien, Diane M

    2008-11-01

    Food web studies based on stable C and N isotope ratios usually assume isotopic equilibrium between a consumer and its diet. In the Arctic, strong seasonality in food availability often leads to diet switching, resulting in a consumer's isotopic composition to be in flux between different food sources. Experimental work investigating the time course and dynamics of isotopic change in Arctic fauna has been lacking, although these data are crucial for accurate interpretation of food web relationships. We investigated seasonal (ice-covered spring vs. ice-free summer) and temperature (1 vs. 4 degrees C) effects on growth and stable C and N isotopic change in the common nearshore Arctic amphipod Onisimus litoralis following a diet switch and while fasting in the laboratory. In spring we found no significant temperature effect on N turnover [half-life (HL) estimates: HL-N = 20.4 at 4 degrees C, 22.4 days at 1 degrees C] and a nonsignificant trend for faster growth and C turnover at the higher temperature (HL-C = 13.9 at 4 degrees C, 18.7 days at 1 degrees C). A strong seasonal effect was found, with significantly slower growth and C and N turnover in the ice-free summer period (HL-N = 115.5 days, HL-C = 77.0 days). Contrary to previous studies, metabolic processes rather than growth accounted for most of the change in C and N isotopic composition (84-89 and 67-77%, respectively). This study provides the first isotopic change and metabolic turnover rates for an Arctic marine invertebrate and demonstrates the risk of generalizing turnover rates based on taxon, physiology, and environment. Our results highlight the importance of experimental work to determine turnover rates for species of interest.

  20. Reconstruction of trophic pathways between plankton and the North Iberian sardine (Sardina pilchardus using stable isotopes

    Directory of Open Access Journals (Sweden)

    Antonio Bode

    2004-03-01

    Full Text Available Feeding on phyto- and zooplankton by juvenile (< 1 year old and adult sardines (Sardina pilchardus was inferred from analyses of natural abundance of stable carbon and nitrogen isotopes in samples from the northwestern Iberian Peninsula (Spain collected at the beginning of the upwelling season and peak spawning period of sardine. Plankton samples were fractionated through nets of 20, 200, 500, 1000 and 2000 ?m mesh-size and the muscle protein of individual sardines was isolated before isotopic determinations. Up to six planktonic components and two sardine feeding types were identified from the modes in the frequency distributions of isotope abundance values. Also, the most probable pathways for carbon and nitrogen flows between compartments were analysed. The resulting food web revealed a relatively large degree of omnivory, both in plankton and sardine components, which confirms that complex trophic interactions could also occur in pelagic upwelling ecosystems. Young sardines had isotope abundance values clustered around a single mode in the frequency distribution, while adult sardines displayed two main modes. These modes are interpreted as representative of two extreme feeding types: one related to the individual capture of zooplankton prey and the other to unselective filter-feeding. Although both types of feeding could include micro- (20-200 ?m and mesozooplankton (200-2000 ?m prey, phytoplankton appears to be ingested mainly by filter-feeding. However, even adult sardines must be mainly zoophagous to achieve the observed isotopic abundance values, taking into account current assumptions on stable isotope enrichment through trophic levels. From the differences in the resulting pathways using either carbon or nitrogen isotopes, we interpreted that sardines acquire most of the protein nitrogen from zooplankton while a substantial fraction of their carbon would derive from phytoplankton. These interpretations agree with the information

  1. Use of a stable copper isotope (65Cu) in the differential diagnosis of Wilson's disease.

    Science.gov (United States)

    Lyon, T D; Fell, G S; Gaffney, D; McGaw, B A; Russell, R I; Park, R H; Beattie, A D; Curry, G; Crofton, R J; Gunn, I

    1995-06-01

    1. 65Cu/63Cu stable-isotope ratios have been measured in blood serum after oral administration of the stable isotope 65Cu. The incorporation of the isotope into the plasma protein pool was followed at various times for up to 3 days. The resulting patterns of enrichment in healthy control subjects, in Wilson's disease patients and in heterozygotes for the Wilson's disease gene, were similar in appearance to those found by others using copper radioactive isotopes. After an initially high enrichment at 2 h after dosage, the Wilson's disease cases, in contrast to the control subjects, did not show a secondary rise in isotope enrichment of the plasma pool after 72 h, demonstrating a failure to incorporate copper into caeruloplasmin. The Wilson's disease heterozygotes had variable degrees of impairment of isotope incorporation, not always distinguished from those of control subjects. 2. The stability of the isotope also permits the copper tracer to be followed for a longer period. Ten healthy subjects were studied for over 40 days, allowing the biological half-time of an oral dose of copper to be determined (median 18.5 days, 95% confidence interval 14-26 days). Known heterozygotes for the Wilson's disease gene were found to have a significantly increased biological half-time for removal of copper from the plasma pool (median 43 days, 95% confidence interval 32-77 days). 3. The incorporation of 65 Cu in patients with diseases of the liver (other than Wilson's disease) was found to be similar to that in control subjects, aiding differential diagnosis.

  2. Molecular structures of HNC and HCN derived from the eight stable isotopic species. [Transitions

    Energy Technology Data Exchange (ETDEWEB)

    Pearson, E.F.; Creswell, R.A.; Winnewisser, M.; Winnewisser, G.

    1976-01-01

    Ground vibrational state rotational transitions were measured for the following isotopic species of HNC: H/sup 15/NC, H/sup 15/N/sup 13/C, DN/sup 13/C, D/sup 15/NC and D/sup 15/N/sup 13/C. Similar transitions were also measured for H/sup 13/C/sup 15/N and D/sup 13/C/sup 15/N. These measurements complete the set of rotational constants for the eight stable isotopic species of the two molecules. Molecular structures are calculated from these data in several ways and are compared with the results of recent ab initio calculations.

  3. Study on the Effect of the Separating Unit Optimization on the Economy of Stable Isotope Separation

    Directory of Open Access Journals (Sweden)

    YANG Kun

    2015-01-01

    Full Text Available An economic criterion called as yearly net profit of single separating unit (YNPSSU was presented to evaluate the influence of structure optimization on the economy. Using YNPSSU as a criterion, economic analysis was carried out for the structure optimization of separating unit in the case of separating SiF4 to obtain the 28Si and 29Si isotope. YNPSSU was calculated and compared with that before optimization. The results showed that YNPSSU was increased by 12.3% by the structure optimization. Therefore, the structure optimization could increase the economy of the stable isotope separation effectively.

  4. Simulating stable carbon and chlorine isotope ratios in dissolved chlorinated groundwater pollutants with BIOCHLOR-ISO

    Science.gov (United States)

    Höhener, Patrick

    2016-12-01

    BIOCHLOR is a well-known simple tool for evaluating the transport of dissolved chlorinated solvents in groundwater, ideal for rapid screening and teaching. This work extends the BIOCHLOR model for the calculation of stable isotope ratios of carbon and chlorine isotopes in chloroethenes. An exact solution for the three-dimensional reactive transport of a chain of degrading compounds including sorption is provided in a spreadsheet and applied for modeling the transport of individual isotopes 12C, 13C, 35Cl, 37Cl from a constant source. The model can consider secondary isotope effects that can occur in the breaking of Csbnd Cl bonds. The model is correctly reproducing results for δ13C and δ37Cl modeled by a previously published 1-D numerical model without secondary isotope effects, and is also reproducing results from a microcosm experiment with secondary chlorine isotope effects. Two applications of the model using field data from literature are further given and discussed. The new BIOCHLOR-ISO model is distributed as a spreadsheet (MS EXCEL) along with this publication.

  5. Stable isotope ratios in meteoric recharge and groundwater at Mt. Vulture volcano, southern Italy

    Science.gov (United States)

    Paternoster, M.; Liotta, M.; Favara, R.

    2008-01-01

    SummaryA rain gauge network consisting of five sites located at different altitudes, ranging from 320 to 1285 m.a.s.l., was installed at Mt. Vulture volcano (southern Italy). Rain water samples were collected monthly over a two-year period and their isotopic composition (δ 18O and δD) was analyzed. During the same period, circulating groundwater was sampled from 24 springs and wells distributed throughout the study area. Monthly isotopic composition values were used to determine the local meteoric water line (LMWL). Its slope is slightly lower than the relationship defined by Longinelli and Selmo (Longinelli, A., Selmo, E., 2003. Isotopic composition of precipitation in Italy: a first overall map. J. Hydrol. 270, 75-88) for southern Italy. The groundwater samples analyzed were distributed essentially along the LMWL. The weighted local meteoric water line (WLMWL), defined through the mean values weighted by the rainfall amount, however, may define in a short range the meteoric end-member in the local hydrological cycle more precisely. Since most of the groundwater sampling locations do not show seasonal variations in their stable isotope values, the flow system appears to be relatively homogeneous. The mean altitude of the recharge by rainfall infiltration was estimated on the basis of the local vertical isotopic gradient δ 18O. A few springs, which show anomalous isotopic values, reveal more regional circulation systems, associated with tectonic structures responsible for the ascent of deeper water.

  6. Insights into Ancient Human Populations and their Environment through Stable Isotope Analysis

    Science.gov (United States)

    Macko, S. A.

    2011-12-01

    Fundamental to the understanding of human history is the ability to make interpretations based on artifacts and other remains which are used to gather information about an ancient population. Sequestered in the organic matrices of these remains can be information concerning incidence of disease, population interactions, genetic defects and diet. Stable isotopes have long been used to interpret diet and trophic interactions in modern ecosystems. We suggest that the isotope compositions of a commonly overlooked material, human hair, is an ideal tool to be used in gleaning information, especially on human diets, about ancient civilizations. Hair can be well-preserved and is amenable to routine measurements of 13C, 15N and 34S isotope analyses and distinguishing sources of nutrition. We have isotopically characterized hair from both modern and ancient individuals. There is a wide diversity in isotope values owing, at least partially, to the levels of seafood, corn-fed animals and other grains in diet. Using these isotope tracers, new information regarding historical figures (George Washington, 1799 AD) to perhaps the most ancient of mummies, the Chinchorro of Chile (more than 7000 BP) as well as the Moche of Peru (1500 BP) and the best preserved mummy, the Neolithic Ice Man of the Oetztaler Alps (5200 BP), have been deciphered. It appears that the often-overlooked hair in archaeological sites represents a significant approach for understanding ancient human communities and their environments, as well as new perspectives on our use of our own modern nutritional sources.

  7. Identification of methanogenic pathways in anaerobic digesters using stable carbon isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Laukenmann, Stephan; Polag, Daniela; Greule, Markus; Lelieveld, Jos; Keppler, Frank [Max-Planck-Institut fuer Chemie, Mainz (Germany); Heuwinkel, Hauke; Gronauer, Andreas [Landesanstalt fuer Landwirtschaft, Freising (Germany)

    2010-12-15

    In a series of anaerobic batch experiments, the stable carbon isotopes, {delta}{sup 13}C{sub CH4} and {delta}{sup 13}C{sub CO2}, were measured in biogas produced from various sources (maize, cellulose, inoculum) to identify the degradation kinetics and specific methanogenic pathways. Isotopic analysis was performed using a new absorption laser spectrometer in addition to conventional MS. A comparison of the isotopic evolution shows large isotope dynamics for maize and cellulose, indicating a temporal change in degradation pathways and/or a change in the relative contribution from different carbon fractions within the substrate. Further batch experiments with isotopically labelled acetate (either {sup 13}CH{sub 3}CO{sub 2}Na or CH{sup 13}{sub 3}CO{sub 2}Na) were carried out to study the degradation of acetate in inoculum systematically. The results suggest that the acetate is completely oxidized into CO{sub 2} which in turn is partly reduced to CH{sub 4}. Furthermore, the distinct isotopic signature CH{sub 4} and CO{sub 2} (for acetate-methyl labelling as well as for acetate-carboxy labelling) indicate that only a minor part of the produced methane derives from acetate. A substantial fraction of methane may have been produced at an earlier stage of the reaction chain or by other potential methane precursors such as formate or methanol. (Copyright copyright 2010 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim)

  8. STABLE CARBON ISOTOPES OF HCO//3 IN THE AQUIA AQUIFER, MARYLAND: EVIDENCE FOR AN ISOTOPICALLY HEAVY SOURCE OF CO//2.

    Science.gov (United States)

    Chapelle, Francis H.; Knobel, LeRoy L.

    1985-01-01

    Identifying sources and sinks of dissolved inorganic carbon species is an important step in understanding the geochemistry of ground-water systems. This is particularly important for Atlantic Coastal Plain aquifers because bicarbonate (HCO//3** minus ) is frequently the major dissolved anion. The purpose of this paper is to document the stable carbon isotope composition of dissolved inorganic carbon in the Aquia aquifer, Maryland, and to use this data to help identify sources and sinks of dissolved HCO//3** minus . Subjects covered include hydrogeology, ground-water chemistry, sources and sinks, and others. Refs.

  9. Comparative Hydrology Over Monsoonal Regions Using Seasonal Distributions of Stable Water Isotopes.

    Science.gov (United States)

    Brown, D. P.; Worden, J.; Noone, D. C.

    2007-12-01

    The hydrologic regimes of monsoonal regions contain complex balances of large-scale advective supply of water, surface exchange and atmospheric condensation, which are important for the regional energy balance and climate. Stable water isotopes are powerful tools for studying such processes, as isotopic fractionations occurring during evaporation and condensation give rise to measurable variations in the isotopic composition that reflects the history of moist processes for each observed air parcel. The HDO/H2O data set from the Tropospheric Emission Spectrometer (TES) on NASA's Aura spacecraft offers a unique global view of the isotopic composition of water vapor. The TES data set, and the analysis here, is complimentary to previous work using isotopic ratios in precipitation; however it need not be that the simple relationships found in the precipitation data hold for the atmospheric vapor case because of the variability induced by atmospheric mixing and convection. Over tropical continents, the intensity of water vapor recycling, precipitation rates and circulation patterns are thought to dominate the seasonal isotopic composition of water vapor and rainfall. By examining and contrasting the isotopic budgets of the Amazon, north Australia, and Asian monsoon regions, we gain insight into these hydrological processes, show which processes are regionally robust, and expose those processes that are regionally unique. To establish the importance of local processes on the regional isotopic composition, we first examine the relationship between the measured isotopic composition and meteorological parameters that capture the strength of the local processes. Secondly, we use the history of condensation, evaporation and air mass mixing during transport from five-day origin locations to the local TES observations, and the isotopic ratios of vapor at both locations, to examine isotopic changes that occur upstream. Using this information, as well as a simple isotopic exchange

  10. Linking cases of illegal shootings of the endangered California condor using stable lead isotope analysis

    Energy Technology Data Exchange (ETDEWEB)

    Finkelstein, Myra E., E-mail: myraf@ucsc.edu [Microbiology and Environmental Toxicology Department, University of California, Santa Cruz, CA 95064 (United States); Kuspa, Zeka E. [Microbiology and Environmental Toxicology Department, University of California, Santa Cruz, CA 95064 (United States); Welch, Alacia [National Park Service, Pinnacles National Park, 5000 Highway 146, Paicines, CA 95043 (United States); Eng, Curtis; Clark, Michael [Los Angeles Zoo and Botanical Gardens, 5333 Zoo Drive, Los Angeles, CA 90027 (United States); Burnett, Joseph [Ventana Wildlife Society, 19045 Portola Dr. Ste. F-1, Salinas, CA 93908 (United States); Smith, Donald R. [Microbiology and Environmental Toxicology Department, University of California, Santa Cruz, CA 95064 (United States)

    2014-10-15

    Lead poisoning is preventing the recovery of the critically endangered California condor (Gymnogyps californianus) and lead isotope analyses have demonstrated that ingestion of spent lead ammunition is the principal source of lead poisoning in condors. Over an 8 month period in 2009, three lead-poisoned condors were independently presented with birdshot embedded in their tissues, evidencing they had been shot. No information connecting these illegal shooting events existed and the timing of the shooting(s) was unknown. Using lead concentration and stable lead isotope analyses of feathers, blood, and recovered birdshot, we observed that: i) lead isotope ratios of embedded shot from all three birds were measurably indistinguishable from each other, suggesting a common source; ii) lead exposure histories re-constructed from feather analysis suggested that the shooting(s) occurred within the same timeframe; and iii) two of the three condors were lead poisoned from a lead source isotopically indistinguishable from the embedded birdshot, implicating ingestion of this type of birdshot as the source of poisoning. One of the condors was subsequently lead poisoned the following year from ingestion of a lead buckshot (blood lead 556 µg/dL), illustrating that ingested shot possess a substantially greater lead poisoning risk compared to embedded shot retained in tissue (blood lead ∼20 µg/dL). To our knowledge, this is the first study to use lead isotopes as a tool to retrospectively link wildlife shooting events. - Highlights: • We conducted a case-based analysis of illegal shootings of California condors. • Blood and feather Pb isotopes were used to reconstruct the illegal shooting events. • Embedded birdshot from the three condors had the same Pb isotope ratios. • Feather and blood Pb isotopes indicated that the condors were shot in a common event. • Ingested shot causes substantially greater lead exposure compared to embedded shot.

  11. Impact of bioavailability restrictions on microbially induced stable isotope fractionation. 1. Theoretical calculation.

    Science.gov (United States)

    Thullner, Martin; Kampara, Makeba; Richnow, Hans H; Harms, Hauke; Wick, Lukas Y

    2008-09-01

    The microbial degradation of organic substrates often exhibits a fractionation of stable isotopes which leads to an enrichment of the heavier isotope in the remaining substrate. The use of this effect to quantify the amount of biodegraded substrate in contaminated aquifers requires that the isotope fractionation factor is constant in time and space. In many natural and engineered systems the bioavailable concentration at the location of the enzymes differs from the average bulk concentration of the substrate. When enzymatically driven substrate degradation is coupled to a preceding transport step controlling the bioavailability of the substrate, the observed isotope fractionation becomes a function of the bulk substrate concentration. The sensitivity of the observed isotope fractionation factor toward such substrate concentration changes depends on the ratio of bulk substrate concentration and Michaelis-Menten constant and on the ratio between the specific affinity of the microorganisms toward the substrate and the first order rate constant of the bioavailability limiting transport process. Highest sensitivities toward substrate concentration were found for combinations of high substrate concentration with low substrate bioavailability (i.e., high ratios of substrate concentration and Michaelis-Menten constant, and high ratios of specific affinity and transport rate constant). As a consequence, changes in concentration and isotopic composition of a bioavailability limited substrate in batch experiments should not exhibit a linear relation in a Rayleigh plot, and the slope of the Rayleigh plot should show a decreasing trend with concentration decrease. When using isotope fractionation to quantify biodegradation along groundwater flow paths, changes in observed isotope fractionation might occur while contaminant concentration decreases along a flow path.

  12. Joint interpretation of enantiomer and stable isotope fractionation for chiral pesticides degradation

    DEFF Research Database (Denmark)

    Jin, Biao; Rolle, Massimo

    2016-01-01

    introduce a modeling approach with the aim of unifying and integrating the interpretation of isotopic and enantiomeric fractionation. The model is based on the definition of enantiomer-specific isotopologues and jointly predicts the evolution of concentration, enantiomer fractionation, as well as changes...... in stable isotope ratios of different elements. The method allows evaluating different transformation pathways and was applied to investigate enzymatic degradation of dichlorprop (DCPP), enzymatic degradation of mecoprop methyl ester (MCPPM), and microbial degradation of α-hexachlorocyclohexane (α......-HCH) by different bacterial strains and under different redox conditions. The model accurately reproduces the isotopic and enantiomeric data observed in previous experimental studies and precisely captures the dual-dimensional trends characterizing different reaction pathways. Furthermore, the model allows testing...

  13. Stable isotope dilution analysis of hydrologic samples by inductively coupled plasma mass spectrometry

    Science.gov (United States)

    Garbarino, J.R.; Taylor, H.E.

    1987-01-01

    Inductively coupled plasma mass spectrometry is employed in the determination of Ni, Cu, Sr, Cd, Ba, Ti, and Pb in nonsaline, natural water samples by stable isotope dilution analysis. Hydrologic samples were directly analyzed without any unusual pretreatment. Interference effects related to overlapping isobars, formation of metal oxide and multiply charged ions, and matrix composition were identified and suitable methods of correction evaluated. A comparability study snowed that single-element isotope dilution analysis was only marginally better than sequential multielement isotope dilution analysis. Accuracy and precision of the single-element method were determined on the basis of results obtained for standard reference materials. The instrumental technique was shown to be ideally suited for programs associated with certification of standard reference materials.

  14. Application of stable isotopes and hydrochemical analysis in groundwater aquifers of Argolis Peninsula (Greece).

    Science.gov (United States)

    Matiatos, Ioannis; Alexopoulos, Apostolos

    2011-12-01

    The present study examines the isotopic and hydrochemical composition of 18 inland spring waters and 3 coastal karstic spring waters, covering the period between October 2005 and March 2008. The stable isotopes ((18)O, (2)H) processing has revealed the absence of significant evaporation phenomena and that the origin of fresh water samples is meteoric. Using (18)O values in rainfall waters, an average line of isotopic depletion with altitude has been constructed, extracting a rate of-0.45‰/100 m as typical for the study area. Furthermore, the mean altitude of recharge of the springs has been estimated by plotting the groundwater sampling points on a δ(18)O versus altitude diagram. Hydrochemistry results have shown that the dissolution of carbonate, flysch and ophiolitic formations defines the hydrochemical characteristics of groundwater. Moreover, seawater intrusion in the coastal area is significantly high, causing the water in the three karstic springs to be brackish.

  15. Separation of selected stable isotopes by liquid-phase thermal diffusion and by chemical exchange

    Science.gov (United States)

    Rutherford, W. M.; Jepson, B. E.; Michaels, E. D.

    Useful applications of enriched stable nuclides are unduly restricted by high cost and limited availability. Recent research on liquid phase thermal diffusion (LTD) has resulted in practical processes for separating S34, CL35, and CL37 in significant quantities (100 to 500 g/yr) at costs much lower than those associated with the electromagnetic (Calutron) process. The separation of the isotopes of bromine by LTD is now in progress and BR79 is being produced in relatively simple equivalent at a rate on the order of 0.5 g/day. The results of recent measurements show that the isotopes of Zn can be separated by LTD of zinc alkyls. The isotopes of calcium can be separated by LTD and by chemical exchange. The LTD process is based on the use of aqueous Ca(NO3)2 as a working fluid.

  16. Stable Isotope Quantitative N-Glycan Analysis by Liquid Separation Techniques and Mass Spectrometry.

    Science.gov (United States)

    Mittermayr, Stefan; Albrecht, Simone; Váradi, Csaba; Millán-Martín, Silvia; Bones, Jonathan

    2017-01-01

    Liquid phase separation analysis and subsequent quantitation remains a challenging task for protein-derived oligosaccharides due to their inherent structural complexity and diversity. Incomplete resolution or co-detection of multiple glycan species complicates peak area-based quantitation and associated statistical analysis when optical detection methods are used. The approach outlined herein describes the utilization of stable isotope variants of commonly used fluorescent tags that allow for mass-based glycan identification and relative quantitation following separation by liquid chromatography (LC) or capillary electrophoresis (CE). Comparability assessment of glycoprotein-derived oligosaccharides is performed by derivatization with commercially available isotope variants of 2-aminobenzoic acid or aniline and analysis by LC- and CE-mass spectrometry. Quantitative information is attained from the extracted ion chromatogram/electropherogram ratios generated from the light and heavy isotope clusters.

  17. Linking carbon and water cycles using stable isotopes across scales: progress and challenges

    Directory of Open Access Journals (Sweden)

    C. Werner

    2011-03-01

    Full Text Available Stable isotope analysis is a powerful tool for tracing biogeochemical processes in the carbon and water cycles. One particularly powerful approach is to employ multiple isotopes where the simultaneous assessment of the D/H,18O/16O and/or 13C/12C in different compounds provide a unique means to investigate the coupling of water and carbon fluxes at various temporal and spatial scales. Here, we present a research update on recent advances in our process-based understanding of the utilization of carbon, oxygen and hydrogen isotopes to lend insight into carbon and water cycling. We highlight recent technological developments and approaches, their strengths and methodological precautions with examples covering scales from minutes to centuries and from the leaf to the globe.

  18. Application of non-traditional stable isotopes in analytical ecogeochemistry assessed by MC ICP-MS--A critical review.

    Science.gov (United States)

    Irrgeher, Johanna; Prohaska, Thomas

    2016-01-01

    Analytical ecogeochemistry is an evolving scientific field dedicated to the development of analytical methods and tools and their application to ecological questions. Traditional stable isotopic systems have been widely explored and have undergone continuous development during the last century. The variations of the isotopic composition of light elements (H, O, N, C, and S) have provided the foundation of stable isotope analysis followed by the analysis of traditional geochemical isotope tracers (e.g., Pb, Sr, Nd, Hf). Questions in a considerable diversity of scientific fields have been addressed, many of which can be assigned to the field of ecogeochemistry. Over the past 15 years, other stable isotopes (e.g., Li, Zn, Cu, Cl) have emerged gradually as novel tools for the investigation of scientific topics that arise in ecosystem research and have enabled novel discoveries and explorations. These systems are often referred to as non-traditional isotopes. The small isotopic differences of interest that are increasingly being addressed for a growing number of isotopic systems represent a challenge to the analytical scientist and push the limits of today's instruments constantly. This underlines the importance of a metrologically sound concept of analytical protocols and procedures and a solid foundation of data processing strategies and uncertainty considerations before these small isotopic variations can be interpreted in the context of applied ecosystem research. This review focuses on the development of isotope research in ecogeochemistry, the requirements for successful detection of small isotopic shifts, and highlights the most recent and innovative applications in the field.

  19. Surface oceanographic fronts influencing deep-sea biological activity: Using fish stable isotopes as ecological tracers

    Science.gov (United States)

    Louzao, Maite; Navarro, Joan; Delgado-Huertas, Antonio; de Sola, Luis Gil; Forero, Manuela G.

    2017-06-01

    Ecotones can be described as transition zones between neighbouring ecological systems that can be shaped by environmental gradients over a range of space and time scales. In the marine environment, the detection of ecotones is complex given the highly dynamic nature of marine systems and the paucity of empirical data over ocean-basin scales. One approach to overcome these limitations is to use stable isotopes from animal tissues since they can track spatial oceanographic variability across marine systems and, in turn, can be used as ecological tracers. Here, we analysed stable isotopes of deep-sea fishes to assess the presence of ecological discontinuities across the western Mediterranean. We were specifically interested in exploring the connection between deep-sea biological activity and particular oceanographic features (i.e., surface fronts) occurring in the pelagic domain. We collected samples for three different abundant deep-sea species in May 2004 from an experimental oceanographic trawling cruise (MEDITS): the Mictophydae jewel lanternfish Lampanyctus crocodilus and two species of the Gadidae family, the silvery pout Gadiculus argenteus and the blue whiting Micromesistius poutassou. The experimental survey occurred along the Iberian continental shelf and the upper and middle slopes, from the Strait of Gibraltar in the SW to the Cape Creus in the NE. The three deep-sea species were highly abundant throughout the study area and they showed geographic variation in their isotopic values, with decreasing values from north to south disrupted by an important change point around the Vera Gulf. Isotopic latitudinal gradients were explained by pelagic oceanographic conditions along the study area and confirm the existence of an ecotone at the Vera Gulf. This area could be considered as an oceanographic boundary where waters of Atlantic origin meet Mediterranean surface waters forming important frontal structures such as the Almeria-Oran front. In fact, our results

  20. A refined sampling strategy for intra-tooth stable isotope analysis of mammalian enamel

    Science.gov (United States)

    Zazzo, A.; Bendrey, R.; Vella, D.; Moloney, A. P.; Monahan, F. J.; Schmidt, O.

    2012-05-01

    Serial sampling and stable isotope analysis performed along the growth axis of vertebrate tooth enamel records differences attributed to seasonal variation in diet, climate or animal movement. Because several months are required to obtain mature enamel in large mammals, modifications in the isotopic composition of environmental parameters are not instantaneously recorded, and stable isotope analysis of tooth enamel returns a time-averaged signal attenuated in its amplitude relative to the input signal. For convenience, stable isotope profiles are usually determined on the side of the tooth where enamel is thickest. Here we investigate the possibility of improving the time resolution by targeting the side of the tooth where enamel is thinnest. Observation of developing third molars (M3) in sheep shows that the tooth growth rate is not constant but decreases exponentially, while the angle between the first layer of enamel deposited and the enamel-dentine junction increases as a tooth approaches its maximal length. We also noted differences in thickness and geometry of enamel growth between the mesial side (i.e., the side facing the M2) and the buccal side (i.e., the side facing the cheek) of the M3. Carbon and oxygen isotope variations were measured along the M3 teeth from eight sheep raised under controlled conditions. Intra-tooth variability was systematically larger along the mesial side and the difference in amplitude between the two sides was proportional to the time of exposure to the input signal. Although attenuated, the mesial side records variations in the environmental signal more faithfully than the buccal side. This approach can be adapted to other mammals whose teeth show lateral variation in enamel thickness and could potentially be used as an internal check for diagenesis.

  1. Stable isotopic composition of perchlorate and nitrate accumulated in plants: Hydroponic experiments and field data

    Science.gov (United States)

    Estrada, Nubia Luz; Böhlke, John Karl; Sturchio, Neil C.; Gu, Baohua; Harvey, Greg; Burkey, Kent O.; Grantz, David A.; McGrath, Margaret T.; Anderson, Todd A.; Rao, Balaji; Sevanthi, Ritesh; Hatzinger, Paul B.; Jackson, W. Andrew

    2017-01-01

    Natural perchlorate (ClO4−) in soil and groundwater exhibits a wide range in stable isotopic compositions (δ37Cl, δ18O, and Δ17O), indicating that ClO4− may be formed through more than one pathway and/or undergoes post-depositional isotopic alteration. Plants are known to accumulate ClO4−, but little is known about their ability to alter its isotopic composition. We examined the potential for plants to alter the isotopic composition of ClO4− in hydroponic and field experiments conducted with snap beans (Phaseolus vulgaris L.). In hydroponic studies, anion ratios indicated that ClO4− was transported from solutions into plants similarly to NO3− but preferentially to Cl− (4-fold). The ClO4− isotopic compositions of initial ClO4− reagents, final growth solutions, and aqueous extracts from plant tissues were essentially indistinguishable, indicating no significant isotope effects during ClO4− uptake or accumulation. The ClO4− isotopic composition of field-grown snap beans was also consistent with that of ClO4− in varying proportions from irrigation water and precipitation. NO3− uptake had little or no effect on NO3− isotopic compositions in hydroponic solutions. However, a large fractionation effect with an apparent ε (15N/18O) ratio of 1.05 was observed between NO3− in hydroponic solutions and leaf extracts, consistent with partial NO3− reduction during assimilation within plant tissue. We also explored the feasibility of evaluating sources of ClO4− in commercial produce, as illustrated by spinach, for which the ClO4− isotopic composition was similar to that of indigenous natural ClO4−. Our results indicate that some types of plants can accumulate and (presumably) release ClO4− to soil and groundwater without altering its isotopic characteristics. Concentrations and isotopic compositions of ClO4−and NO3− in plants may be useful for determining sources of fertilizers and sources of ClO4− in their growth environments and

  2. Stable lead isotopic analyses of historic and contemporary lead contamination of San Francisco Bay estuary

    Science.gov (United States)

    Ritson, P.I.; Bouse, R.M.; Flegal, A.R.; Luoma, S.N.

    1999-01-01

    Variations in stable lead isotopic composition (240Pb, 206Pb, 207Pb, 208Pb) in three sediment cores from the San Francisco Bay estuary document temporal changes in sources of lead during the past two centuries. Sediment, with lead from natural geologic sources, and relatively homogeneous lead isotopic compositions are overlain by sediments whose isotopic compositions indicate change in the sources of lead associated with anthropogenic modification of the estuary. The first perturbations of lead isotopic composition in the cores occur in the late 1800s concordant with the beginning of industrialization around the estuary. Large isotopic shifts, toward lower 206Pb/207Pb, occur after the turn of the century in both Richardson and San Pablo Bays. A similar relationship among lead isotopic compositions and lead concentrations in both Bays suggest contamination from the same source (a lead smelter). The uppermost sediments (post 1980) of all cores also have a relatively homogenous lead isotopic composition distinct from pre-anthropogenic and recent aerosol signatures. Lead isotopic compositions of leachates from fourteen surface sediments and five marsh samples from the estuary were also analyzed. These analyses suggest that the lead isotopic signature identified in the upper horizons of the cores is spatially homogeneous among recently deposited sediments throughout the estuary. Current aerosol lead isotopic compositions [Smith, D.R., Niemeyer, S., Flegal, A.R., 1992. Lead sources to California sea otters: industrial inputs circumvent natural lead biodepletion mechanisms. Environmental Research 57, 163-175] are distinct from the isotopic compositions of the surface sediments, suggesting that the major source of lead is cycling of historically contaminated sediments back through the water column. Both the upper core sediments and surface sediments apparently derive their lead predominantly from sources internal to the estuary. These results support the idea that

  3. Stable carbon isotope fractionation in the UV photolysis of CFC-11 and CFC-12

    Directory of Open Access Journals (Sweden)

    A. Zuiderweg

    2011-12-01

    Full Text Available The chlorofluorocarbons CFC-11 (CCl3F and CFC-12 (CCl2F2 are stable atmospheric compounds that are produced at the earth's surface, but removed only at high altitudes in the stratosphere, where their removal liberates atomic chlorine that then catalytically destroys stratospheric ozone. For such long-lived compounds, isotope effects in the stratospheric removal reactions have a large effect on their global isotope budgets. We have determined the photolytic isotope fractionation for stable carbon isotopes of CFC-11 and CFC-12 in laboratory experiments. 13C/12C isotope fractionations (ϵ range from (−23.7 ± 0.9 to (−17.5 ± 0.4‰ for CFC-11 and (−69.2 ± 3.4 to (−49.4 ± 2.3‰ for CFC-12 between 203 and 288 K, a temperature range relevant to conditions in the troposphere and stratosphere. These results suggest that CFCs should become strongly enriched in 13C with decreasing mixing ratio in the stratosphere, similar to what has been recently observed for CFC chlorine isotopes. In conjunction with the strong variations in CFC emissions before and after the Montréal Protocol, the stratospheric enrichments should also lead to a significant temporal increase in the 13C content of the CFCs at the surface over the past decades, which should be recorded in atmospheric air archives such as firn air.

  4. Local vs. Regional Groundwater Flow Delineation from Stable Isotopes at Western North America Springs.

    Science.gov (United States)

    Springer, Abraham E; Boldt, Elizabeth M; Junghans, Katie M

    2017-01-01

    The recharge location for many springs is unknown because they can be sourced from proximal, shallow, atmospheric sources or long-traveled, deep, regional aquifers. The stable isotope ((18) O and (2) H) geochemistry of springs water can provide cost-effective indications of relative flow path distance without the expense of drilling boreholes, conducting geophysical studies, or building groundwater flow models. Locally sourced springs generally have an isotopic signature similar to local precipitation for that region and elevation. Springs with a very different isotopic composition than local meteoric inputs likely have non-local recharge, representing a regional source. We tested this local vs. regional flow derived hypothesis with data from a new, large springs isotopic database from studies across Western North America in Arizona, Nevada, and Alberta. The combination of location-specific precipitation data with stable isotopic groundwater data provides an effective method for flow path determination at springs. We found springs in Arizona issue from a mix of regional and local recharge sources. These springs have a weak elevation trend across 1588 m of elevation where higher elevation springs are only slightly more depleted than low elevation springs with a δ(18) O variation of 5.9‰. Springs sampled in Nevada showed a strong elevation-isotope relationship with high-elevation sites discharging depleted waters and lower elevation springs issuing enriched waters; only a 2.6‰ difference exists in (18) O values over an elevation range of more than 1500 m. Alberta's springs are mostly sourced from local flow systems and show a moderate elevation trend of 1200 m, but the largest range in δ(18) O, 7.1‰.

  5. Principles and limitations of stable isotopes in differentiating organic and conventional foodstuffs: 2. Animal products.

    Science.gov (United States)

    Inácio, Caio T; Chalk, Phillip M

    2017-01-02

    In this review, we examine the variation in stable isotope signatures of the lighter elements (δ(2)H, δ(13)C, δ(15)N, δ(18)O, and δ(34)S) of tissues and excreta of domesticated animals, the factors affecting the isotopic composition of animal tissues, and whether stable isotopes may be used to differentiate organic and conventional modes of animal husbandry. The main factors affecting the δ(13)C signatures of livestock are the C3/C4 composition of the diet, the relative digestibility of the diet components, metabolic turnover, tissue and compound specificity, growth rate, and animal age. δ(15)N signatures of sheep and cattle products have been related mainly to diet signatures, which are quite variable among farms and between years. Although few data exist, a minor influence in δ(15)N signatures of animal products was attributed to N losses at the farm level, whereas stocking rate showed divergent findings. Correlations between mode of production and δ(2)H and δ(18)O have not been established, and only in one case of an animal product was δ(34)S a satisfactory marker for mode of production. While many data exist on diet-tissue isotopic discrimination values among domesticated animals, there is a paucity of data that allow a direct and statistically verifiable comparison of the differences in the isotopic signatures of organically and conventionally grown animal products. The few comparisons are confined to beef, milk, and egg yolk, with no data for swine or lamb products. δ(13)C appears to be the most promising isotopic marker to differentiate organic and conventional production systems when maize (C4) is present in the conventional animal diet. However, δ(13)C may be unsuitable under tropical conditions, where C4 grasses are abundant, and where grass-based husbandry is predominant in both conventional and organic systems. Presently, there is no universal analytical method that can be applied to differentiate organic and conventional animal products.

  6. Stable isotope evidence for trophic niche partitioning in a South African savanna rodent community

    Institute of Scientific and Technical Information of China (English)

    Jacqueline CODRON; Kevin J DUFFY; Nico L AVENANT; Matt SPONHEIMER; Jennifer LEICHLITER; Oli