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Sample records for henn raave karin

  1. Teenager vaatab tagasi / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    2000-01-01

    Eesti Arhitektuurimuuseumi asutamisest ja kümneaastasest tegevusest. Arhitektuurimuuseum asutati 1. I 1991. a. Initsiatiivgruppi kuulusid Karin Hallas (tulevane direktor), Krista Kodres ja Mart Kalm. 7. VI 1996. a. avanäitus Rotermanni soolalaos

  2. The Spin Vector of (832) Karin

    Science.gov (United States)

    Slivan, Stephen M.; Molnar, L. A.

    2010-10-01

    We observed rotation lightcurves of Koronis family and Karin cluster member (832) Karin during its four consecutive apparitions in 2006-2009, and combined the new observations with previously published lightcurves to determine its spin vector orientation and preliminary model shape. Karin is a prograde rotator with a period of 18.352 h, spin obliquity near 41°, and pole ecliptic longitude near either 51° or 228°. Although the two ambiguous pole solutions are near the clustered pole solutions of four Koronis family members whose spins are thought to be trapped in a spin-orbit resonance (Vokrouhlický et al., 2003), Karin does not seem to be trapped in the resonance; this is consistent with the expectation that the 6 My age of Karin (Nesvorný et al., 2002) is too young for YORP torques to have modified its spin since its formation. The spin vector and shape results for Karin will constrain family formation models that include spin properties, and we discuss the Karin results in the context of the other members of the Karin cluster, the Karin parent body, and the parent body's siblings in the Koronis family.

  3. Moving ring reactor 'Karin-1'

    International Nuclear Information System (INIS)

    1983-12-01

    The conceptual design of a moving ring reactor ''Karin-1'' has been carried out to advance fusion system design, to clarify the research and development problems, and to decide their priority. In order to attain these objectives, a D-T reactor with tritium breeding blanket is designed, a commercial reactor with net power output of 500 MWe is designed, the compatibility of plasma physics with fusion engineering is demonstrated, and some other guideline is indicated. A moving ring reactor is composed mainly of three parts. In the first formation section, a plasma ring is formed and heated up to ignition temperature. The plasma ring of compact torus is transported from the formation section through the next burning section to generate fusion power. Then the plasma ring moves into the last recovery section, and the energy and particles of the plasma ring are recovered. The outline of a moving ring reactor ''Karin-1'' is described. As a candidate material for the first wall, SiC was adopted to reduce the MHD effect and to minimize the interaction with neutrons and charged particles. The thin metal lining was applied to the SiC surface to solve the problem of the compatibility with lithium blanket. Plasma physics, the engineering aspect and the items of research and development are described. (Kako, I.)

  4. Spin vectors in the Koronis family: III. (832) Karin

    Science.gov (United States)

    Slivan, Stephen M.; Molnar, Lawrence A.

    2012-08-01

    Studies of asteroid families constrain models of asteroid collisions and evolution processes, and the Karin cluster within the Koronis family is among the youngest families known (Nesvorný, D., Bottke, Jr., W.F., Dones, L., Levison, H.F. [2002]. Nature 417, 720-722). (832) Karin itself is by far the largest member of the Karin cluster, thus knowledge of Karin's spin vector is important to constrain family formation and evolution models that include spin, and to test whether its spin properties are consistent with the Karin cluster being a very young family. We observed rotation lightcurves of Karin during its four consecutive apparitions in 2006-2009, and combined the new observations with previously published lightcurves to determine its spin vector orientation and preliminary model shape. Karin is a prograde rotator with a period of (18.352 ± 0.003) h, spin obliquity near (42 ± 5)°, and pole ecliptic longitude near either (52 ± 5)° or (230 ± 5)°. The spin vector and shape results for Karin will constrain models of family formation that include spin properties; in the meantime we briefly discuss Karin's own spin in the context of those of other members of the Karin cluster and the parent body's siblings in the Koronis family.

  5. Bosnia ja Kosovo riigiloomise õppetunnid / Karin Maandi

    Index Scriptorium Estoniae

    Maandi, Karin

    2005-01-01

    OSCE missiooni koosseisus Kosovos ning Bosnias ja Hertsegoviinas viibinud Karin Maandi kirjeldab aspekte, mis on olnud olulised stabiilsuse tagamisel Balkanil ja Afganistanis. Autori sõnul ei võta välismaailm konflikti sekkudes tihti arvesse, et konfliktist on puudutatud paljud valdkonnad ja et pakutud lahendused peavad sobima kohalikesse oludesse. Lisa: Õppetunnid

  6. Kuldmedal metsmaasikatele ehk austusavaldus Linnele / Karin Truverk

    Index Scriptorium Estoniae

    Truverk, Karin

    2007-01-01

    Chelsea Flower Show'l kuldmedali pälvinud rootsi maastikuarhitekti Ulf Nordfjelli kujundatud ideeaiast "Austusavaldus Linnele". Erilisele kohale on tõstetud Karl von Linne lemmiktaimed: kirju liilia, metspipar ja harakkuljus. Metallplaatide autoriks on kunstnik Anne-Karin Furunes. Projekt, 6 värv. fotot

  7. An efficient rHSV-based complementation system for the production of multiple rAAV vector serotypes.

    Science.gov (United States)

    Kang, W; Wang, L; Harrell, H; Liu, J; Thomas, D L; Mayfield, T L; Scotti, M M; Ye, G J; Veres, G; Knop, D R

    2009-02-01

    Recombinant herpes simplex virus type 1 (rHSV)-assisted recombinant adeno-associated virus (rAAV) vector production provides a highly efficient and scalable method for manufacture of clinical grade rAAV vectors. Here, we present an rHSV co-infection system for rAAV production, which uses two ICP27-deficient rHSV constructs, one bearing the rep2 and cap (1, 2 or 9) genes of rAAV, and the second bearing an AAV2 ITR-gene of interest (GOI) cassette. The optimum rAAV production parameters were defined by producing rAAV2/GFP in HEK293 cells, yielding greater than 9000 infectious particles per cell with a 14:1 DNase resistance particle to infectious particle (DRP/ip) ratio. The optimized co-infection parameters were then used to generate large-scale stocks of rAAV1/AAT, which encode the human alpha-1-antitrypsin (hAAT) protein, and purified by column chromatography. The purified vector was extensively characterized by rAAV- and rHSV-specific assays and compared to transfection-made vector for in vivo efficacy in mice through intramuscular injection. The co-infection method was also used to produce rAAV9/AAT for comparison to rAAV1/AAT in vivo. Intramuscular administration of 1 x 10(11) DRP per animal of rHSV-produced rAAV1/AAT and rAAV9/AAT resulted in hAAT protein expression of 5.4 x 10(4) and 9.4 x 10(5) ng ml(-1) serum respectively, the latter being clinically relevant.

  8. Eesti puitarhitektuuri näitus Rotermanni soolalaos / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    1999-01-01

    1. VII-5. IX avatud näituse 'Eesti puitarhitektuur' koostasid Karin Hallas, Epi Tohvri, helitausta kujundas Ralf Tamm. Näitus on Eesti Arhitektuurimuuseumi ja Riigi Muinsuskaitseinspektsiooni ühisprojekt ning osa UNESCO 'Põhjamaade puitlinnade' programmist.

  9. Precision Deployable Mast for the SWOT KaRIn Instrument

    Data.gov (United States)

    National Aeronautics and Space Administration — Design and prototype a lightweight, precision-deployable mast for the Ka-band Radar Interferometer (KaRIn) antennas in the Surface Water and Ocean Topography (SWOT)...

  10. Work praises the one who has done it : bibliography prize to Karin Maria Rooleid / Kalju Tammaru

    Index Scriptorium Estoniae

    Tammaru, Kalju, 1956-

    2004-01-01

    Tallinna Pedagoogikaülikooli Akadeemilises Raamatukogus toimunud 3. bibliograafiapäeval valiti auhinna vääriliseks Karin Ribenise (Karin Maria Rooleiu) koostatud "Eesti rahvaluule bibliograafia 1993-2000"

  11. Immune responses to rAAV6: The influence of canine parvovirus vaccination and neonatal administration of viral vector

    Directory of Open Access Journals (Sweden)

    Andrea L H Arnett

    2011-11-01

    Full Text Available Recombinant adeno-associated viral (rAAV vectors promote long-term gene transfer in many animal species. Significant effort has focused on the evaluation of rAAV delivery and the immune response in both murine and canine models of neuromuscular disease. However, canines provided for research purposes are routinely vaccinated against canine parvovirus (CPV. rAAV and CPV possess significant homology and are both parvoviruses. Thus, any immune response generated to CPV vaccination has the potential to cross-react with rAAV vectors. In this study, we investigated the immune response to rAAV6 delivery in a cohort of CPV-vaccinated canines and evaluated multiple vaccination regimens in a mouse model of CPV-vaccination. We show that CPV-vaccination stimulates production of neutralizing antibodies with minimal cross-reactivity to rAAV6. In addition, no significant differences were observed in the magnitude of the rAAV6-directed immune response between CPV-vaccinated animals and controls. Moreover, CPV-vaccination did not inhibit rAAV6-mediated transduction. We also evaluated the immune response to early rAAV6-vaccination in neonatal mice. The influence of maternal hormones and cytokines leads to a relatively permissive state in the neonate. We hypothesized that immaturity of the immune system would permit induction of tolerance to rAAV6 when delivered during the neonatal period. Mice were vaccinated with rAAV6 at 1 or 5 days of age, and subsequently challenged with rAAV6 exposure during adulthood via two sequential IM injections, one month apart. All vaccinated animals generated a significant neutralizing antibody response to rAAV6-vaccination that was enhanced following IM injection in adulthood. Taken together, these data demonstrate that the immune response raised against rAAV6 is distinct from that which is elicited by the standard parvoviral vaccines and is sufficient to prevent stable tolerization in neonatal mice.

  12. Microbiological quality of karin butter, a traditionally manufactured butter from Turkey

    Energy Technology Data Exchange (ETDEWEB)

    Gokce, R.; Aslanalp, Y.; Nur Herken, E.

    2010-07-01

    Karin butter, which is characterized by being packaged into sheep or goat rumen (karin), is a traditional butter type. Karin used as a packaging material in the butter production and is an important factor for the butter to be more aromatic and delicious. In this study, karin butter samples collected from factories and local markets were investigated for total aerobic mesophilic bacteria, coliform bacteria, Staphilococcus aureus and mould-yeast counts. The results were evaluated with respect to the legal stipulations of Turkey and other scientific studies related to the subject. The microbiological quality of karin butter samples was found to be poor in general. (Author)

  13. Molecular design for recombinant adeno-associated virus (rAAV) vector production.

    Science.gov (United States)

    Aponte-Ubillus, Juan Jose; Barajas, Daniel; Peltier, Joseph; Bardliving, Cameron; Shamlou, Parviz; Gold, Daniel

    2018-02-01

    Recombinant adeno-associated virus (rAAV) vectors are increasingly popular tools for gene therapy applications. Their non-pathogenic status, low inflammatory potential, availability of viral serotypes with different tissue tropisms, and prospective long-lasting gene expression are important attributes that make rAAVs safe and efficient therapeutic options. Over the last three decades, several groups have engineered recombinant AAV-producing platforms, yielding high titers of transducing vector particles. Current specific productivity yields from different platforms range from 10 3 to 10 5 vector genomes (vg) per cell, and there is an ongoing effort to improve vector yields in order to satisfy high product demands required for clinical trials and future commercialization.Crucial aspects of vector production include the molecular design of the rAAV-producing host cell line along with the design of AAV genes, promoters, and regulatory elements. Appropriately, configuring and balancing the expression of these elements not only contributes toward high productivity, it also improves process robustness and product quality. In this mini-review, the rational design of rAAV-producing expression systems is discussed, with special attention to molecular strategies that contribute to high-yielding, biomanufacturing-amenable rAAV production processes. Details on molecular optimization from four rAAV expression systems are covered: adenovirus, herpesvirus, and baculovirus complementation systems, as well as a recently explored yeast expression system.

  14. Comparison of Serum rAAV Serotype-Specific Antibodies in Patients with Duchenne Muscular Dystrophy, Becker Muscular Dystrophy, Inclusion Body Myositis, or GNE Myopathy.

    Science.gov (United States)

    Zygmunt, Deborah A; Crowe, Kelly E; Flanigan, Kevin M; Martin, Paul T

    2017-09-01

    Recombinant adeno-associated virus (rAAV) is a commonly used gene therapy vector for the delivery of therapeutic transgenes in a variety of human diseases, but pre-existing serum antibodies to viral capsid proteins can greatly inhibit rAAV transduction of tissues. Serum was assayed from patients with Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), inclusion body myositis (IBM), and GNE myopathy (GNE). These were compared to serum from otherwise normal human subjects to determine the extent of pre-existing serum antibodies to rAAVrh74, rAAV1, rAAV2, rAAV6, rAAV8, and rAAV9. In almost all cases, patients with measurable titers to one rAAV serotype showed titers to all other serotypes tested, with average titers to rAAV2 being highest in all instances. Twenty-six percent of all young normal subjects (18 years old). Fifty percent of all IBM and GNE patients also had antibody titers to all rAAV serotypes, while only 18% of DMD and 0% of BMD patients did. In addition, serum-naïve macaques treated systemically with rAAVrh74 could develop cross-reactive antibodies to all other serotypes tested at 24 weeks post treatment. These data demonstrate that most DMD and BMD patients should be amenable to vascular rAAV-mediated treatment without the concern of treatment blockage by pre-existing serum rAAV antibodies, and that serum antibodies to rAAVrh74 are no more common than those for rAAV6, rAAV8, or rAAV9.

  15. Stranding of two sperm whales (Physeter macrocephalus) in the "North Sea trap" at Henne Strand, Denmark

    DEFF Research Database (Denmark)

    Hansen, Mette Sif; Alstrup, Aage K. O.; Hansen, Jørgen H.

    2016-01-01

    In February 2014 two male sperm whales (Physeter macrocephalus) stranded at Henne Strand, Denmark. One whale (MCE 1644) was found dead, while the other (MCE 1645) was still alive, but drowned during the high tide. To increase our knowledge of sperm whales, conduct forage investigations, post......-mortem and diagnostic examinations were carried out. The decay of the carcasses progressed quickly. The whales had large (MCE 1644) or moderate (MCE 1645) numbers of squid beaks (Gonatus fabricii) in the stomachventricles, but no evidence of recentfresh feeding. Both whales had acute dermatitis probably due to trauma...... severe localized or systemic infections. The finding of large volumes of bloody pleural fluid with large quantities of C. septicum suggests that MCE 1644 died of infection. However, reservations must be taken due to the pronounced decay of the carcass. Sperm whales have strong social bonds where...

  16. Tuisland. Karin Brynard. Kaapstad: Penguin Ran- dom House Suid ...

    African Journals Online (AJOL)

    29 Nov 2016 ... Tuisland (2016) is oud-joernalis Karin. Brynard se derde spanningsroman. Haar debuut, Plaasmoord (2009), is met die. UJ-debuutprys bekroon en haar tweede boek, Onse Vaders (2012), het die ATKV- prosaprys verower. Beide Plaasmoord. (2009) en Onse Vaders (2012) is ook met die M-Net-filmprys ...

  17. Euroopa aadel armastab ajalugu / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2004-01-01

    Organisatsioonist Europa Nostra, mille nõukokku valiti 2003. a. Karin Hallas. Tänavuste Europa Nostra preemiate kätteandmisest Münchenis ja objektidest, mille hulgas oli Leedu endise historitsistliku valitsushoone renoveerimine Kaunases (nüüd ülikooli rektoraat). Uue ilme saanud Tallinna raekoja esitamisest kandidaadiks

  18. The Mediation of Fandom in Karin Giphart's Maak me blij.

    NARCIS (Netherlands)

    Lamerichs, N.A.S.

    2012-01-01

    Abstract:The plot of the Dutch novel Maak me blij (Make me happy) (2005) by Karin Giphart draws from the culture of online fan communities. It describes the life of a lesbian in her late 20s, Ziggy, who has a terminally ill mother. Ziggy is an active fan who writes and reads femmeslash fan

  19. Deletion of the B-B' and C-C' regions of inverted terminal repeats reduces rAAV productivity but increases transgene expression.

    Science.gov (United States)

    Zhou, Qingzhang; Tian, Wenhong; Liu, Chunguo; Lian, Zhonghui; Dong, Xiaoyan; Wu, Xiaobing

    2017-07-14

    Inverted terminal repeats (ITRs) of the adeno-associated virus (AAV) are essential for rescue, replication, packaging, and integration of the viral genome. While ITR mutations have been identified in previous reports, we designed a new truncated ITR lacking the B-B' and C-C' regions named as ITRΔBC and investigated its effects on viral genome replication, packaging, and expression of recombinant AAV (rAAV). The packaging ability was compared between ITRΔBC rAAV and wild-type (wt) ITR rAAV. Our results showed the productivity of ITRΔBC rAAV was reduced 4-fold, which is consistent with the 8-fold decrease in the replication of viral genomic DNA of ITRΔBC rAAV compared with wt ITR rAAV. Surprisingly, transgene expression was significantly higher for ITRΔBC rAAV. A preliminary exploration of the underlying mechanisms was carried out by inhibiting and degrading the ataxia telangiectasia mutated (ATM) protein and the Mre11 complex (MRN), respectively, since the rAAV expression was inhibited by the ATM and/or MRN through cis interaction or binding with wt ITRs. We demonstrated that the inhibitory effects were weakened on ITRΔBC rAAV expression. This study suggests deletion in ITR can affect the transgene expression of AAV, which provides a new way to improve the AAV expression through ITRs modification.

  20. Modernism and Metamorphosis: Karin Kiwus' Das Chinesische Examen

    Directory of Open Access Journals (Sweden)

    James Rolleston

    1997-01-01

    Full Text Available A Chinese examination requires one to record everything felt or recalled within a given time frame. It "tests" an entire life. Karin Kiwus' poetic tools for taking the exam are monumentality, the freezing of imagined history into the dimension of a statue—that then crumbles back into time; and metamorphosis, the subjection of moments and personae to quasi-musical structures of ceaseless variation.

  1. Supramolecular polypseudorotaxane gels for controlled delivery of rAAV vectors in human mesenchymal stem cells for regenerative medicine.

    Science.gov (United States)

    Rey-Rico, Ana; Babicz, Heiko; Madry, Henning; Concheiro, Angel; Alvarez-Lorenzo, Carmen; Cucchiarini, Magali

    2017-10-15

    The aim of this work was to investigate, for the first time, the possibility of using supramolecular polypseudorotaxane gels as scaffolds that can durably deliver rAAV vectors for applications in cartilage regeneration. Dispersions of Pluronic ® F68 (PF68) or Tetronic ® 908 (T908) containing either hyaluronic acid (HA) or chondroitin sulfate (CS) were prepared in PBS. Then, alpha-cyclodextrin (αCD) was added to some dispersions to form polypseudorotaxane gels. Polysaccharides and αCD reinforced the viscoelasticity of the gels, which could withstand autoclaving without changes. In vitro release of rAAV vectors and subsequent transduction of human mesenchymal stem cells (hMSCs) by rAAV vectors from the release medium and from gels in direct contact with the cells were investigated. Compared with free vectors, the gels provided higher levels of transgene expression. CS (or HA)/PF68/αCD gels rapidly released rAAV vectors while CS (or HA)/T908/αCD gels provided sustained release probably due to different interactions with the viral vectors. Incorporation of αCD into CS (or HA)/PF68 gels resulted on higher rAAV concentrations and sustained levels of transgene expression over time. HA increased the bioactivity and cytocompatibility of the gels, especially those based on T908. Overall, combining rAAV gene transfer with polypseudorotaxane gels may provide new, promising tools for human tissue engineering and regenerative medicine strategies. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Differential impact of the HEN1 homolog HENN-1 on 21U and 26G RNAs in the germline of Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Leonie M Kamminga

    Full Text Available RNA interference (RNAi-related pathways affect gene activity by sequence-specific recruitment of Ago proteins to mRNA target molecules. The sequence specificity of this process stems from small RNA (sRNA co-factors bound by the Ago protein. Stability of sRNA molecules in some pathways is in part regulated by Hen1-mediated methylation of their 3' ends. Here we describe the effects of the Caenorhabditis elegans HEN1 RNA-methyl-transferase homolog, HENN-1, on the different RNAi pathways in this nematode. We reveal differential effects of HENN-1 on the two pathways that are known to employ methylated sRNA molecules: the 26G and 21U pathways. Surprisingly, in the germline, stability of 21U RNAs, the C. elegans piRNAs, is only mildly affected by loss of methylation; and introduction of artificial 21U target RNA does not further destabilize non-methylated 21U RNAs. In contrast, most 26G RNAs display reduced stability and respond to loss of HENN-1 by displaying increased 3'-uridylation frequencies. Within the 26G RNA class, we find that specifically ERGO-1-bound 26G RNAs are modified by HENN-1, while ALG-3/ALG-4-bound 26G RNAs are not. Global gene expression analysis of henn-1 mutants reveals mild effects, including down-regulation of many germline-expressed genes. Our data suggest that, apart from direct effects of reduced 26G RNA levels of henn-1 on gene expression, most effects on global gene expression are indirect. These studies further refine our understanding of endogenous RNAi in C. elegans and the roles for Hen1 like enzymes in these pathways.

  3. [Karin Hallas. Das Tallinner Mietshaus. Vom Historismus bis zum Jugendstil] / Paul Kaegbein

    Index Scriptorium Estoniae

    Kaegbein, Paul

    2006-01-01

    Arvustus: Karin Hallas. Das Tallinner Mietshaus. Vom Historismus bis zum Jugendstil. In: Architektur und bildende Kunst im Baltikum um 1900. Frankfurt am Main, Berlin, Bern, Bruxelles, New York, Wien: Lang 1999. S. 173-192.

  4. rAAV Vectors as Safe and Efficient Tools for the Stable Delivery of Genes to Primary Human Chondrosarcoma Cells In Vitro and In Situ

    Directory of Open Access Journals (Sweden)

    Henning Madry

    2012-01-01

    Full Text Available Treatment of chondrosarcoma remains a major challenge in orthopaedic oncology. Gene transfer strategies based on recombinant adenoassociated viral (rAAV vectors may provide powerful tools to develop new, efficient therapeutic options against these tumors. In the present study, we tested the hypothesis that rAAV is adapted for a stable and safe delivery of foreign sequences in human chondrosarcoma tissue by transducing primary human chondrosarcoma cells in vitro and in situ with different reporter genes (E. coli lacZ, firefly luc, Discosoma sp. RFP. The effects of rAAV administration upon cell survival and metabolic activities were also evaluated to monitor possibly detrimental effects of the gene transfer method. Remarkably, we provide evidence that efficient and prolonged expression of transgene sequences via rAAV can be safely achieved in all the systems investigated, demonstrating the potential of the approach of direct application of therapeutic gene vectors as a means to treat chondrosarcoma.

  5. Longevity of rAAV vector and plasmid DNA in blood after intramuscular injection in nonhuman primates: implications for gene doping.

    Science.gov (United States)

    Ni, W; Le Guiner, C; Gernoux, G; Penaud-Budloo, M; Moullier, P; Snyder, R O

    2011-07-01

    Legitimate uses of gene transfer technology can benefit from sensitive detection methods to determine vector biodistribution in pre-clinical studies and in human clinical trials, and similar methods can detect illegitimate gene transfer to provide sports-governing bodies with the ability to maintain fairness. Real-time PCR assays were developed to detect a performance-enhancing transgene (erythropoietin, EPO) and backbone sequences in the presence of endogenous cellular sequences. In addition to developing real-time PCR assays, the steps involved in DNA extraction, storage and transport were investigated. By real-time PCR, the vector transgene is distinguishable from the genomic DNA sequence because of the absence of introns, and the vector backbone can be identified by heterologous gene expression control elements. After performance of the assays was optimized, cynomolgus macaques received a single dose by intramuscular (IM) injection of plasmid DNA, a recombinant adeno-associated viral vector serotype 1 (rAAV1) or a rAAV8 vector expressing cynomolgus macaque EPO. Macaques received a high plasmid dose intended to achieve a significant, but not life-threatening, increase in hematocrit. rAAV vectors were used at low doses to achieve a small increase in hematocrit and to determine the limit of sensitivity for detecting rAAV sequences by single-step PCR. DNA extracted from white blood cells (WBCs) was tested to determine whether WBCs can be collaterally transfected by plasmid or transduced by rAAV vectors in this context, and can be used as a surrogate marker for gene doping. We demonstrate that IM injection of a conventional plasmid and rAAV vectors results in the presence of DNA that can be detected at high levels in blood before rapid elimination, and that rAAV genomes can persist for several months in WBCs.

  6. Detection of the YORP effect for small asteroids in the Karin family

    Science.gov (United States)

    Nesvorny, David; Carruba, Valerio; Vokrouhlicky, David

    2016-10-01

    The Karin family formed by a collisional breakup of a ~40-km parent asteroid only 5.75 Myr ago. The young age can be demonstrated by numerically integrating the orbits of Karin family members backward in time and showing the convergence of orbital elements. Previous work has pointed out that the convergence is not ideal if the backward integration only accounts for the gravitational perturbations from the Solar System planets. It improves when the thermal radiation force known as the Yarkovsky effect is accounted for. This method can be used to estimate the spin obliquities of Karin family members. Here we show that the obliquity distribution of diameter D=1-2 km asteroids in the Karin family is bimodal, as expected if the YORP effect acted to move obliquities toward extreme values (0 or 180 deg). The measured magnitude of the effect is consistent with the standard YORP model. Specifically, the strength of the YORP effect is inferred to be roughly 70% of the nominal YORP strength obtained for a collection of random Gaussian spheroids. The surface thermal conductivity is found to be 0.07-0.2 W/m/K (thermal inertia 300-500 in the SI units). These results are consistent with surfaces composed of rough and rocky regolith. The obliquity values predicted here for 480 members of the Karin cluster can be validated by the lightcurve inversion method. In broader context, the bimodal distribution of obliquities in the Karin cluster can be thought as an initial stage of dynamical evolution that later leads to a characteristically bi-lobed distribution of family members in the semimajor axis (e.g., Eos, Merxia or Erigone families).

  7. Detection of the YORP Effect for Small Asteroids in the Karin Cluster

    Science.gov (United States)

    Carruba, V.; Nesvorný, D.; Vokrouhlický, D.

    2016-06-01

    The Karin cluster is a young asteroid family thought to have formed only ≃ 5.75 Myr ago. The young age can be demonstrated by numerically integrating the orbits of Karin cluster members backward in time and showing the convergence of the perihelion and nodal longitudes (as well as other orbital elements). Previous work has pointed out that the convergence is not ideal if the backward integration only accounts for the gravitational perturbations from the solar system planets. It improves when the thermal radiation force known as the Yarkovsky effect is accounted for. This argument can be used to estimate the spin obliquities of the Karin cluster members. Here we take advantage of the fast growing membership of the Karin cluster and show that the obliquity distribution of diameter D≃ 1{--}2 km Karin asteroids is bimodal, as expected if the YORP effect acted to move obliquities toward extreme values (0° or 180°). The measured magnitude of the effect is consistent with the standard YORP model. The surface thermal conductivity is inferred to be 0.07-0.2 W m-1 K-1 (thermal inertia ≃ 300{--}500 J m-2 K-1 s{}-1/2). We find that the strength of the YORP effect is roughly ≃ 0.7 of the nominal strength obtained for a collection of random Gaussian spheroids. These results are consistent with a surface composed of rough, rocky regolith. The obliquity values predicted here for 480 members of the Karin cluster can be validated by the light-curve inversion method.

  8. The mediation of fandom in Karin Giphart's Maak me blij

    Directory of Open Access Journals (Sweden)

    Nicolle Lamerichs

    2012-09-01

    Full Text Available The plot of the Dutch novel Maak me blij (Make me happy (2005 by Karin Giphart draws from the culture of online fan communities. It describes the life of a lesbian in her late 20s, Ziggy, who has a terminally ill mother. Ziggy is an active fan who writes and reads femmeslash fan fiction—that is, lesbian interpretations of characters from mainstream series such as Star Trek: Voyager (1995–2001. By providing through Ziggy a personal view of fan communities and the genres that flourish there, Maak me blij connects the romantic motives of original lesbian fiction with its underground sister, fan fiction. The novel draws from various source texts and illustrates how fans interpret texts within a wider literary landscape. I use the concept of intermediality to analyze how Maak me blij mediates different types of original fiction (lesbian romances, science fiction and fan fiction (femmeslash, Star Trek fan fiction to establish new views on fandom and its construction of gender and intimacy. These motives are not only apparent within the text itself but also within the character of Ziggy as a fan writer with her own original alien characters.

  9. Eesti funktsionalismi näitus Jyväskyläs / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2002-01-01

    6. VIII-15. IX Alvar Aalto muuseumis Eesti Arhitekuurimuuseumi näitus "Funktsionalism Eestis". Eksponeeritakse 1930. aastate originaalprojekte, digitaliseeritud ajaloolisi fotosid ning makette. Näituse ja kataloogi on koostanud Karin Hallas-Murula, näituse kujundanud Peeter Mauer, kataloogi kujundanud Jaanus Tamme ja Jaak Peep (Tank)

  10. 1960. aastate arhitektuurist, ajast ja vaimust / Udo Ivask ; interv. Karin Hallas-Murula ja Reet Varblane

    Index Scriptorium Estoniae

    Ivask, Udo, 1932-2017

    2007-01-01

    1960. aastatel moodsaid hooneid projekteerinud arhitekti Udo Ivaskiga vestlevad 2. septembrini Eesti Arhitektuurimuuseumis avatud näituse "Kuuekümnendad. Eesti moodne arhitektuur" kuraator Karin Hallas-Murula ja "Sirbi" toimetaja Reet Varblane. Udo Ivaskist (sünd. 1932), tema ehitisi

  11. Remodelling of human osteoarthritic cartilage by FGF-2, alone or combined with Sox9 via rAAV gene transfer.

    Science.gov (United States)

    Cucchiarini, Magali; Terwilliger, Ernest F; Kohn, Dieter; Madry, Henning

    2009-08-01

    Compensating for the loss of extracellular cartilage matrix, as well as counteracting the alterations of the chondrocyte phenotype in osteoarthritis are of key importance to develop effective therapeutic strategies against this disorder. In the present study, we analysed the benefits of applying a potent gene combination to remodel human osteoarthritic (OA) cartilage. We employed the promising recombinant adeno-associated virus (rAAV) vector to deliver the mitogenic fibroblast growth factor 2 (FGF-2) factor, alone or simultaneously with the transcription factor Sox9 as a key activator of matrix synthesis, to human normal and OA articular chondrocytes. We evaluated the effects of single (FGF-2) or combined (FGF-2/SOX9) transgene expression upon the regenerative activities of chondrocytes in three dimensional cultures in vitro and in cartilage explants in situ. Single overexpression of FGF-2 enhanced the survival and proliferation of both normal and OA chondrocytes, without stimulating the matrix synthetic processes in the increased pools of cells. The mitogenic properties of FGF-2 were maintained when SOX9 was co-overexpressed and concomitant with an increase in the production of proteoglycans and type-II collagen, suggesting that the transcription factor was capable of counterbalancing the effects of FGF-2 on matrix accumulation. Also important, expression of type-X collagen, a marker of hypertrophy strongly decreased following treatment by the candidate vectors. Most remarkably, the levels of activities achieved in co-treated human OA cartilage were similar to or higher than those observed in normal cartilage. The present findings show that combined expression of candidate factors in OA cartilage can re-establish key features of normal cartilage and prevent the pathological shift of metabolic homeostasis. These data provide further motivation to develop coupled gene transfer approaches via rAAV for the treatment of human OA.

  12. Testing the Deployment Repeatability of a Precision Deployable Boom Prototype for the Proposed SWOT Karin Instrument

    Science.gov (United States)

    Agnes, Gregory S.; Waldman, Jeff; Hughes, Richard; Peterson, Lee D.

    2015-01-01

    NASA's proposed Surface Water Ocean Topography (SWOT) mission, scheduled to launch in 2020, would provide critical information about Earth's oceans, ocean circulation, fresh water storage, and river discharge. The mission concept calls for a dual-antenna Ka-band radar interferometer instrument, known as KaRIn, that would map the height of water globally along two 50 km wide swaths. The KaRIn antennas, which would be separated by 10 meters on either side of the spacecraft, would need to be precisely deployable in order to meet demanding pointing requirements. Consequently, an effort was undertaken to design build and prototype a precision deployable Mast for the KaRIn instrument. Each mast was 4.5-m long with a required dilitation stability of 2.5 microns over 3 minutes. It required a minimum first mode of 7 Hz. Deployment repeatability was less than +/- 7 arcsec in all three rotation directions. Overall mass could not exceed 41.5 Kg including any actuators and thermal blanketing. This set of requirements meant the boom had to be three times lighter and two orders of magnitude more precise than the existing state of the art for deployable booms.

  13. Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes.

    Science.gov (United States)

    Frisch, Janina; Orth, Patrick; Rey-Rico, Ana; Venkatesan, Jagadeesh Kumar; Schmitt, Gertrud; Madry, Henning; Kohn, Dieter; Cucchiarini, Magali

    2017-11-01

    Implantation of peripheral blood aspirates induced towards chondrogenic differentiation upon genetic modification in sites of articular cartilage injury may represent a powerful strategy to enhance cartilage repair. Such a single-step approach may be less invasive than procedures based on the use of isolated or concentrated MSCs, simplifying translational protocols in patients. In this study, we provide evidence showing the feasibility of overexpressing the mitogenic and pro-anabolic insulin-like growth factor I (IGF-I) in human peripheral blood aspirates via rAAV-mediated gene transfer, leading to enhanced proliferative and chondrogenic differentiation (proteoglycans, type-II collagen, SOX9) activities in the samples relative to control (reporter rAAV-lacZ) treatment over extended periods of time (at least 21 days, the longest time-point evaluated). Interestingly, IGF-I gene transfer also triggered hypertrophic, osteo- and adipogenic differentiation processes in the aspirates, suggesting that careful regulation of IGF-I expression may be necessary to contain these events in vivo. Still, the current results demonstrate the potential of targeting human peripheral blood aspirates via therapeutic rAAV transduction as a novel, convenient tool to treat articular cartilage injuries. © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

  14. Ton H.M. van Schaik and Karin Strengers-Olde Kalter, Het Arme Roomse Leven. Geschiedenis van de katholieke caritas in de stad Utrecht

    Directory of Open Access Journals (Sweden)

    Joost van Genabeek

    2017-07-01

    Full Text Available Ton H.M. van Schaik and Karin Strengers-Olde Kalter, Het Arme Roomse Leven. Geschiedenis van de katholieke caritas in de stad Utrecht (Hilversum: Verloren, 2016, 276 pp., isbn 978 90 8704 578 4.

  15. Transcriptome Profiling of Neovascularized Corneas Reveals miR-204 as a Multi-target Biotherapy Deliverable by rAAVs

    Directory of Open Access Journals (Sweden)

    Yi Lu

    2018-03-01

    Full Text Available Corneal neovascularization (NV is the major sight-threatening pathology caused by angiogenic stimuli. Current drugs that directly target pro-angiogenic factors to inhibit or reverse the disease require multiple rounds of administration and have limited efficacies. Here, we identify potential anti-angiogenic corneal microRNAs (miRNAs and demonstrate a framework that employs discovered miRNAs as biotherapies deliverable by recombinant adeno-associated viruses (rAAVs. By querying differentially expressed miRNAs in neovascularized mouse corneas induced by alkali burn, we have revealed 39 miRNAs that are predicted to target more than 5,500 differentially expressed corneal mRNAs. Among these, we selected miR-204 and assessed its efficacy and therapeutic benefit for treating injured corneas. Our results show that delivery of miR-204 by rAAV normalizes multiple novel target genes and biological pathways to attenuate vascularization of injured mouse cornea. Importantly, this gene therapy treatment alternative is efficacious and safe for mitigating corneal NV. Overall, our work demonstrates the discovery of potential therapeutic miRNAs in corneal disorders and their translation into viable treatment alternatives.

  16. Uhked naised / Kozik, Eerika; Tomingas, Triin; Sepp, Karin; Liik, Kadi; Kliimask, Katrin; Saunpere, Anne ; tekst Kärt Kross

    Index Scriptorium Estoniae

    2007-01-01

    Mille üle oled enda juures tõeliselt uhke, mida võid enda kiituseks öelda? Küsimusele vastavad Starland Salongi omanik ja Loyd Tootmine OÜ osanik Eerika Kozik, psühholoog Triin Tomingas, Kontuur Leo Burnett projektidirektor Karin Sepp, Tallinna Ülikooli õppejõud Kadi Liik, AS Koolibri turundusjuht Katrin Kliimask ja eraettevõtja Anne Saunpere

  17. Effective genetic modification and differentiation of hMSCs upon controlled release of rAAV vectors using alginate/poloxamer composite systems.

    Science.gov (United States)

    Díaz-Rodríguez, P; Rey-Rico, A; Madry, H; Landin, M; Cucchiarini, M

    2015-12-30

    Viral vectors are common tools in gene therapy to deliver foreign therapeutic sequences in a specific target population via their natural cellular entry mechanisms. Incorporating such vectors in implantable systems may provide strong alternatives to conventional gene transfer procedures. The goal of the present study was to generate different hydrogel structures based on alginate (AlgPH155) and poloxamer PF127 as new systems to encapsulate and release recombinant adeno-associated viral (rAAV) vectors. Inclusion of rAAV in such polymeric capsules revealed an influence of the hydrogel composition and crosslinking temperature upon the vector release profiles, with alginate (AlgPH155) structures showing the fastest release profiles early on while over time vector release was more effective from AlgPH155+PF127 [H] capsules crosslinked at a high temperature (50°C). Systems prepared at room temperature (AlgPH155+PF127 [C]) allowed instead to achieve a more controlled release profile. When tested for their ability to target human mesenchymal stem cells, the different systems led to high transduction efficiencies over time and to gene expression levels in the range of those achieved upon direct vector application, especially when using AlgPH155+PF127 [H]. No detrimental effects were reported on either cell viability or on the potential for chondrogenic differentiation. Inclusion of PF127 in the capsules was also capable of delaying undesirable hypertrophic cell differentiation. These findings are of promising value for the further development of viral vector controlled release strategies. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Smart and Controllable rAAV Gene Delivery Carriers in Progenitor Cells for Human Musculoskeletal Regenerative Medicine with a Focus on the Articular Cartilage.

    Science.gov (United States)

    Rey-Rico, Ana; Cucchiarini, Magali

    2017-01-01

    Cell therapy using mesenchymal stem cells (MSCs) is a powerful tool for the treatment of various diseases and injuries. Still, important limitations including the large amounts of cells required for application in vivo and the age-related decline in lifespan, proliferation, and potency may hinder the use of MSCs in patients. In this regard, gene therapy may offer strong approaches to optimize the use of MSCs for regenerative medicine. Diverse nonviral and viral gene vehicles have been manipulated to genetically modify MSCs, among which the highly effective and relatively safe recombinant adeno-associated viral (rAAV) vectors that emerged as the preferred gene delivery system to treat human disorders. Yet, clinical adaptation of such gene vehicles may be limited by several hurdles, including the possibility of dissemination to nontarget sites and the presence of immune and toxic responses in the host organism that may impair their therapeutic actions. The use of smart biomaterials acting as interfaces to enhance the temporal and spatial presentation of therapeutic agents in the target place and/or acting as scaffolding for MSC growth is an innovative, valuable approach to overcome these shortcomings that else restrain the efficacy of such potent cell populations. Here, we provide an overview on the most recent tissue engineering approaches based on the use of biomaterials acting as vehicles for rAAV vectors to target MSCs directly in the recipient (in vivo strategy) or as supportive matrices for rAAV-modified MSCs for indirect cell reimplantation (ex vivo strategy) as means to activate the reparative processes in tissues of the musculoskeletal system. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  19. Overcoming Otherness. Considerations on Intercultural Aspects in Karin Gündisch’s Novels

    Directory of Open Access Journals (Sweden)

    Petra-Melitta Roșu

    2015-09-01

    Full Text Available Immigration, cultural identity, integration, tolerance and the ability to adapt to a new environment are issues that often come up in today’s global society. The paper focuses on the way in which cultural otherness is perceived by children and teenagers. The article is based on the analysis of Karin Gündisch’s novels. The awarded author from a migrant background offers an insight into the above mentioned problems. Gündisch’s characters are mostly East-Europeans or South-East-Europeans who try to make a living in developed countries of the Western World. The author portrays entire families, children, parents and grandparents. Thus we can discuss the different stages of integration and the different attitudes towards it. How does the comfort of “home” influence identity? How can you rediscover yourself abroad? Does cultural diversity increase prejudice? What does the idea of a “Paradise abroad” involve? These are some of the aspects, the article is trying to explore.

  20. Review: Karin Reiber (2005. Organisation im Spiegel der Regula Benedicti [Organisation in Reflection of Regula Benedicti

    Directory of Open Access Journals (Sweden)

    Jürgen Rausch

    2007-01-01

    Full Text Available For 1500 years, the monastic life of the Benedictines has been defined by rules dating from the time of the first foundation of the order, suggesting an organizational structure that has outlasted historical upheavals and changes. Starting from a new understanding of organisational development as organisational learning, Karin REIBER has looked for clues to organisational formation and learning through a hermeneutic textual analysis of the Regula Benedicti of the 6th Century. Within a theoretical framework, REIBER develops three dimensions of organisational formation and three stages of organisational learning. In a historical reconstruction, the author analyses the text of the Regula Benedicti. In a second section, REIBER adapts and interprets the results of the hermeneutic analysis through a double three-stage theoretical model. She works out interesting aspects for organisational learning, which relate individual learning to organisational learning in favour of members of the organisation, for which consideration for individuality forms a part of the leadership concept and as a framework for social relationships, establishing an informal organisational structure beside the formal one. This illuminating work leads to an integrated concept of organisational learning, which extends the notion of tradition as a characteristic feature of an innovative organisation and which, through continuity and topicality, can hence guarantee the continued existence of modern organisations in the future. URN: urn:nbn:de:0114-fqs070174

  1. Kumutekitav arhitektuuriaasta 2005 = The Kumu-lative year in architecture 2005 / Liina Jänes, Karin Paulus

    Index Scriptorium Estoniae

    Jänes, Liina, 1977-

    2006-01-01

    Eesti Kultuurkapitali aastapreemia 2005 - Kumu Kunstimuuseum, arhitekt Pekka Vapaavuori. Objektipreemia - büroohoone City Plaza - Alver Trummal Arhitektid (Andres Alver, Tiit Trummal, Martin Melioranski, Indrek Rünkla). Arhitektuuri ja sisekujunduse parima terviku preemia - Jõelähtme golfiklubi, arhitekt Andres Siim, sisearhitekt Juta Lember. Tegevuaspreemia - Teddy Böckler, Tallinna raekoja pikaajalise uurimis- ja restaureerimistöö, raamatu "Tallinna raekoda : uurimine ja restaureerimine 1952-2004" eest. Teadustöö preemia - Karin Hallas-Murula, kahe eesti-soome teemalise arhitektuuriuurimuse eest: "Suurlinn Tallinn: Eliel Saarineni "Suur-Tallinna" projekt, 1913" ja "Soome-Eesti - sajand arhitektuurisuhteid"

  2. Dokumentaalfilm "Maile Grünbergi värvilised maailmad" = "Maile Grünberg's colourful worlds": a documentary / Karin Paulus, Priit Põldme

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2015-01-01

    Sisearhitekt Maile Grünbergist ja 2014. aastal temast valminud dokumentaalfilmist "Maile Grünbergi värvilised maailmad". Režissöör Peeter Brambat, tegevprodutsent Priit Põldme, käsikiri Karin Paulus, Peeter Brambat, Priit Põldme. 2014. aasta Kultuurkapitali Arhitektuuripreemia kandidaat

  3. [Valitsusliidus olevad Reformierakond ning Isamaa ja Res Publica Liit on kavandatava koolireformi suhtes eri meelt] / Mailis Reps, Tiiu Kuurme, Karin Kütt

    Index Scriptorium Estoniae

    Reps, Mailis, 1975-

    2010-01-01

    Reformierakonna ettepaneku kohta jätta omavalitsustele õiguse otsustada, kas gümnaasium ja põhikool lahutatakse või jätkatakse ühendkooliga avaldavad arvamust riigikogu liige Mailis Reps, Tallinna Ülikooli dotsent Tiiu Kuurme ja Rannu keskkooli direktor Karin Kütt

  4. Reseñas y Recensiones: Lenis, Karin. (2014). El sistema de responsabilidad penal de menores. Un estudio de las legislaciones de España y Colombia desde la teoría del Derecho Penal del Enemigo.

    OpenAIRE

    Muñetones Rozo, Ingrid Bibiana

    2015-01-01

    Reseñas y Recensiones: Reseñas y Recensiones: Lenis, Karin. (2014). El sistema de responsabilidad penal de menores. Un estudio de las legislaciones de España y Colombia desde la teoría del Derecho Penal del Enemigo. Bogotá: Grupo Editorial Ibañez, 644 p.

  5. [Mõistete seletused] / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1985-01-01

    Agression lk. 30-31, expulsion lk. 305-306, federation lk. 313-314, human rights, international protection lk. 366-368, international and municipal law, lk. 389-390, intervention lk. 398-399, political crimes (F. J. M. Feldbrugge, revised by H.-J. Uibopuu) lk. 596-597, propaganda, anti-soviet (F. J. M. Feldbrugge, revised by H.-J. Uibopuu) lk. 627-628, separation of powers lk. 695

  6. Rezension von: Karin Schwiter: Lebensentwürfe. Junge Erwachsene im Spannungsfeld zwischen Individualität und Geschlechternormen. Frankfurt am Main u.a.: Campus Verlag 2011.

    Directory of Open Access Journals (Sweden)

    Heike Kahlert

    2011-10-01

    Full Text Available Karin Schwiter untersucht in der vorliegenden Studie, wie junge Erwachsene über ihre Zukunftspläne sprechen und welche Vorstellungen bezüglich ihrer Zukunft sie dabei entwerfen. Die Untersuchung zeichnet sich gegenüber älteren Forschungen zu diesem Thema unter anderem aus durch einen Geschlechtervergleich, die raumzeitliche Fokussierung auf die Deutschschweiz sowie ein diskursanalytisches Vorgehen in Anlehnung an Foucault. Das Ergebnis ist ein anregendes Buch, in dem die Autorin auf der Basis der Erzählungen von 24 jungen Frauen und Männern gesellschaftstheoretische Deutungen und Ausblicke entwickelt. Diese belegen, wie sich neoliberale Ideen von Individualität und fortbestehende Geschlechternormen miteinander so verschränken, dass Thematisierungen geschlechtlicher Ungleichheit kaum mehr möglich scheinen.

  7. Suurlinlik lukshotell / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2007-01-01

    Historitsistlikust hoonest Tallinna vanalinnas Vene tn. 9 (1874. a.), arhitekt Peter Schreiberg. Apteegi tänavale jäävast juurdeehitisest, arhitekt Martin Aunin, sisekujundajad Külli Salum ja Riina Harik

  8. Moblaga Veneetsiasse! / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2006-01-01

    Veneetsia X arhitektuuribiennaalil esindab Eestit arhitektuuribüroo Urban Mark (arhitektid Ülar Mark, Kaja Pae, Indrek Tiigi) ja Tartu Ülikooli Geograafia Instituudi (teadurid Rein Ahas, Anto Aasa, Siiri Silm) projekt. Kommenteerib Ülar Mark. Lühidalt ka teistest eestisisesel konkursil osalenud töödest

  9. Anneteparaad III / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2008-01-01

    Tutvustatakse Tartu Kõrgema Kunstikooli diplomandide lõputöid: 1. Diana Hints "Ba-Rokk", 2. Maire Rebina "Alleshoitud naeratused", 3. Liis Pihlik "Unistused ehk hingele pai", 4. Ille Agu "Pitsiring - pruudipesu kollektsioon", 5. Ingwald Koser "Chaise Longue", 6. Kaisa Paluoja installatsioon "/....../", 7. Kaidi Ploomipuu arvutiga juhitavatel telgedel kootud sisustuskangas

  10. Bruno 2016 / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2016-01-01

    Valik disainiauhinnale Bruno 2016 kandideerivatest esemetest (Gerda Retter "Jääkideta nahadisain", Raul Abner "Kummut Mix", Argo Ader ja Rain Aduson "Fitbi - spordi mugavalt!", Merili Sulg "Seinašabloon Kasemets", Rita Assor "Lugemispesa-mänguala Aas", Mare Kelpman "Terra pleedid", Henri Viljarand "Vineervalamu Gniss")

  11. Saagu valgus / Karin Tarto

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    1998-01-01

    Eesti valgustikujundajatest ja valgustitest. Kas eesti disaineritel on õnnestunud leida oma nišš lambiletil. Tootjatest : OÜ "Raunto", AS "Titanus" (disaineriks Eestis elav rootslane Renni Par)-, Norrast pärineva kontserni "Glamox" tehasest Keilas, "Estoplastist" (disainerid Martin Pärn, Raido Laasing), AS "Steel Line". Valgustikujundajatest Tarmo Luisust, Aleksander Jakovlevist, Raivo Pristolist, Mait Summatavetist, Tõnis Vellamast

  12. Arhitektuurimuuseumid ehitavad / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    2001-01-01

    Arhitektuurimuuseumide konföderatsiooni (ICAM) juhatuse aastakoosolekust Los Angeleses. Uusi ruume saanud või saavaid arhitektuurikeskusi. Uue muuseumikompleksi ehitamist on alustanud New Yorgi Moodsa Kunsti Muuseum (arhitekt Yoshio Taniguchi), 2002. a. valmib Münchenis Moderne Pinakothek (Stephan Braunfels), 2003. a. saab uued ruumid Berlinische Galerie (Frederik Fisher), uue maja Norra Arhitektuurimuuseum (Sverre Fehn), Arhitektuuri- ja Disainimuuseumi konkurss Soomes.

  13. Rokkivad vanadekodud / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2011-01-01

    Vanurite hooldekodude arhitektuurist. Harmoonia Maja (arhitekt Vlad Alexandru Gaivoronschi) Rumeenias, De Rokade põetus- ja hoolduskodu (Arons en Gelauff Architecten) Hollandis, büroo Andrés Jaque Arquitectos kavandatud vanadekodu Hispaanias, Atelier Zündel & Cristea projekteeritud vanadekodu Prantsusmaal

  14. Tokyo / Karin Thea

    Index Scriptorium Estoniae

    Karin, Thea

    2013-01-01

    Tokyo vaatamisväärtused: Sky Tree teletorn, Takeshita Dori jalakäijate tänav, Asakusa linnaosa, The Gate Hotel, Tokyo metroo, Ryogoku Kokugikan, Nippon Kasgaku Miraikan ning Jaapani suurim kalaturg Tsukiji

  15. Põhjamaade aiatrendid / Karin Truverk

    Index Scriptorium Estoniae

    Truverk, Karin

    2007-01-01

    2007. a. märtsis Stockholmis toimunud aiandusmessist Nordiska Trädgardar, mille põhiteemad olid Karl von Linne (1707-1778) ning elu sügis - surm. Rõdude ja kalmude kujundamise võistlusest, kunstnik Stina Sandbergi tööst "Rõõmus surnuaed". 2007. a. püsililleks valiti roog-sinihelmikas, 2006. a. aiaraamatuks Lotte Mölleri teos rootsi maastikuarhitektidest Per Fribergist, Gunnar Martinsonist ja Sven-Ingvar Andersonist "Kolm meistrit, kolm aeda"

  16. Several loadings and stresses of first wall of SiC with metal liner on conceptual design of moving ring reactor 'KARIN-1'

    International Nuclear Information System (INIS)

    Nishikawa, Masahiro; Tachibana, Eizaburo; Watanabe, Kenji; Fujiie, Yoichi.

    1983-01-01

    On conceptual design of moving ring reactor ''KARIN-I'' (Output: 1850 MWe), the first wall of SiC with metal liner is considered by reason that SiC ceramics has specific features of excellent radiation damage resistance in fast neutron spectra and a very low residual radioactivity, and that the thin metal liner has good compatibility with liquid lithium and good vaccum-tight, however, a extent electromagnetic interaction. The electromagnetic force applied on the metal liner and several pressure losses of liquid lithum flow are estimated, and these forces correspond to the fluid mechanical loading on SiC first wall. Thermal loading by neutron flux is calculated on the first wall to obtain temperature distributions along the flow direction and toward the wall thickness. At the outlet of the burning section, the surface temperature of SiC rises to the value of 825 0 C on plasma side and on the metal liner, it rises to the value of 540 0 C. Finally, the stress analysis is performed. The thermal stress is about one order larger than the stress induced by the fluid mechanical loading. At the inlet of the burning section, the average tensile stress of 22.4kg/mm 2 is induced on the outer side of SiC wall, and on the inner side, the average compressive stress of -26.1kg/mm 2 is induced. At the outlet of the burning section, the tensile stress is found to oscillate between 25.5kg/mm 2 and 27.3kg/mm 2 on the outer side of SiC wall by frequency of 1 Hz, and on the inner side, the compressive stress also oscillates between -21.6kg/mm 2 and -29.0kg/mm 2 by the same frequency. These stresses are within the value of fracture stress, (72.5kg/mm 2 ). Difficult residual problems on the first wall are also discussed. (author)

  17. Pargid, maapealsed paradiisid / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2010-01-01

    Arvustus: Park on paradiis looduses ja kunstis / vastutavad toimetajad Mart Külvik, Juhan Maiste. Tartu : Eesti Maaülikool, 2009. Prantsuse ja inglise pargistiilidest, vabakujunduslike parkide jõudmisest Eesti- ja Liivimaale, hiitest ja mõisaparkidest, linnaparkidest

  18. Saksamaa ida kutsub / Thea Karin

    Index Scriptorium Estoniae

    Karin, Thea

    2011-01-01

    Endise Ida-Saksamaa aladel asuvate linnade Dessau-Rosslau ja Chemnitzi (endine Karl-Marx-Stadti) vaatamisväärsustest, s.h UNESCO kultuuripärandisse kuuluvatest Wörlitz-Dessau parkidest ja Bauhausi kompleksist

  19. Kõige kummalisemad elumajad / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2007-01-01

    Le Corbusier' proportsioonisüsteemist "Modulor", Margarete Schütte-Lihotzky ja Peter Eisenmani pakutud uudseid lahendusi arhitektuuris. Mart Kadariku projekteeritud eramust Rannamõisas; arhitektuuribüroo Künnapu & Padrik projekteeritud hoonest Triumph Plaza Tallinnas Narva maanteel; Olev Siinmaa villast Pärnus; püramiidmajadest; Gert Sarve ja Lauri Laisaare projekteeritud monoliitbetoonist eramust Tallinnas Nõmmel; KOKO Arhitektuuristuudios projekteeritud Fahle majast Tallinnas; Ülar Margi ja Indrek Tiigi projekteeritud klaasist seintega majast Tallinnas Silgu tänaval

  20. Tallinna Fotokuu 2015 / Karin Kahre

    Index Scriptorium Estoniae

    Kahre, Karin

    2015-01-01

    Tallinna Fotokuu 2015 programmist. Näitus "Prosu(u)mer" Eesti Kaasaegse Kunsti Muuseumis, kuraator David Raymond Conroy. Näitus "Aegvõte. Time Lapse" Tallinna Kunstihoones, kuraator Anna Laarits. Näitus "Võrdlev sissevaade 1990. aastate üleminekuaja Balti fotokunsti" Tallinna Kunstihoone galeriis, kuraator Tanja Muravskaja. Duo-näitus "Leegi vari" Tartu Kunstimajas, kunstnikud Tarvo Hanno Varres ja Kirke Kangro. Näitus "Plahvatusest tasandikule. Eesti kaasaegne foto 1991-2015" Taru Kunstimuuseumis, kuraator Anneli Porri

  1. Seiklused kapitaguses maailmas / Karin Hansson

    Index Scriptorium Estoniae

    Hansson, Karin

    2007-01-01

    C.S. Lewise 1950.a. ilmunud romaani järgi "Narnia lood : Lõvi, nõid ja riidekapp" ("Chronicles of Narnia : The Lion, the Witch and the Wardrobe") valminud Andrew Adamsoni samanimelisest mängufilmist

  2. Aidsiraport tekitab kahtlusi / Karin Volmer

    Index Scriptorium Estoniae

    Volmer, Karin

    2008-01-01

    Kuigi ÜRO raporti kohaselt on aidsi levik taanduma hakanud, pelgavad eksperdid, et muunduv haigus lööb näilise stabiliseerumise peagi segamini. Eesti aidsi tugikeskuse juhataja Jüri Kalikovi sõnul Eestis alust stabiliseerumisest rääkida ei ole. Lisa: Petlikud numbrid

  3. Rahu, ainult rahu / Karin Bachmann

    Index Scriptorium Estoniae

    Bachmann, Karin, 1976-

    2013-01-01

    Eesti Kunstiakadeemia X Urbanistika ja linnamaastike päevad "Between Architecture of War and Military Urbanism" ("Sõjaarhitektuuri ja militaarurbanistika vahel") 25.-28. aprillil 2013. a. Telliskivi Loomelinnakus Tallinnas. Konverentsi peaesinejad Stephen Graham ja Steven Flusty

  4. Austria peidetud aarded / Thea Karin

    Index Scriptorium Estoniae

    Karin, Thea

    2013-01-01

    Linzi ja Grazi vaatamisväärsustest, arhitektuurist, toidust s.h maailma vanimast tordist - Linzer torte'st. Langenloisis asuvast Wine&Spa Resort Loisium arhitektihotellist, kus Aveda Destination Spas pakutakse koostöös kosmeetikatootja Vinoble Cosmetics'iga viinamarjatoodetel põhinevaid kehahoolitsusi

  5. Ilusa Eesti reklaamikonkurss / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    2009-01-01

    Shanghai 2010. aasta EXPO Eesti paviljoni konkursitööd Eesti Arhitektuurimuuseumis 24. maini. Konkursist, võistlustöödest. Premeeritud tööd. Esimese koha pälvis töö "Savecity.org", autorid Illimar Truverk, Andres Labi, Janno Roos, Ionel Lehari, Priit Hamer ja Kristian Paljasmaa

  6. Hunnitud hütid / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2016-01-01

    Arhitektuuribüroos KOKO Arhitektid (Raivo Kotov, Andrus Kõresaar, Sten-Mark Mändmaa, Indrek Mikk, Martin Tago, Liis Uustal, Maia Grimitliht, Raili Paling, Kadri Kaldam) kavandatud puidust matkahütid Norras Skåpetis

  7. Tailastes kobrutab viha riigijuhi vastu / Karin Dean

    Index Scriptorium Estoniae

    Dean, Karin

    2006-01-01

    Ilmunud ka: Postimees : na russkom jazõke 27. veebr. lk. 11. Tai peaminister Thaksin Shinawatra saatis 24. veebruaril parlamendi laiali ja kuulutas 2. aprilliks välja erakorralised üldvalimised. Lisa: Peaminister

  8. Kahe riigi õpilased elustasid legende / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2010-01-01

    Pärnu Vene Gümnaasium ja Antwerpeni Leonardo Lyceum Belgiast osalevad Comeniuse projektis "Legends Connecting Past and Present, East and West". Etendatakse legende ja muistendeid ning õpitakse eesti ja flaami keelt

  9. Globaalarhitekti uus manifest / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2004-01-01

    Hollandi arhitekt Rem Koolhaas'ist, kelle näitus "Content" Rotterdami Kunstihallis ning näitus "Start" Rotterdami Hollandi Arhitektuuriinstituudis on avatud 31. maini. Näituse "Content" kataloogist. 2000. a. sai R. Koolhaas Pritzkeri preemia, 2003. a. Praemium Imperiale, 2004. a. veebruaris RIBA kuldmedali. Kommenteerinud Andres Alver, Hanno Grossschmidt ja Inga Raukas

  10. Kinoteater tuleb tagasi / Harry Liivrand, Karin Paulus

    Index Scriptorium Estoniae

    Liivrand, Harry, 1961-

    2006-01-01

    Tallinnas Vabaduse väljaku ääres asuvast renoveeritud kino Gloria Palace'i ehk Vene Teatri hoonest (arhitekt Fridrihs Skujinsh, fassaadireljeefide autor Jaan Koort). Renoveerimist juhtisid arhitekt Ra Luhse, sisearhitektid Aivar Oja, Riin Luuk ja Piret Bender ning sisekujundaja Margus Sulengo

  11. Tudengifilmid seavad fookusesse arhitektuuri / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2015-01-01

    Pärnu keskraamatukogus näidatakse Balti Filmi- ja Meediakooli tudengite ühistööna valminud komplekti arhitektuuriteemalistest lühimängufilmidest. Filmid on Kairit Brassi "Lõunavalgus", Liva Vilnite "Sagedus", Marta Pulki "Õhk", Keidi Mäe "Uue alguse loon" ja Epp Kubu "Uustulnuk"

  12. Uudiseid ja meeldetuletusi raamatukogult / Karin Tulve

    Index Scriptorium Estoniae

    Tulve, Karin

    2000-01-01

    2000. aastast on TPÜ raamatukokku ostetud EBSCO online andmebaasid, augustist on võimalik kasutada referaat- ja täistekstandmebaasi Academic Search FullText International 15 CD-ROM-il ja septembrist humanitaarteaduste bibliograafilist andmebaasi Sophia : European Databases in the Humanities CD-ROM-il

  13. Titanic või turist? / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2006-01-01

    Tallinnas Tartu maanteel asuva endise Turisti poe (arhitektid Peep Jänes, Henno Sepmann) asemele tahavad hoone omanikud ehitada kõrghoone nimega Titanic. Hoone ajutine võtmine muinsuskaitse alla on põhjustanud kohtuvaidluse

  14. Oma põlvkonna maskottdisainer / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2005-01-01

    Toivo Raidmetsast (sünd. 1955) ja tema loomingust. Näitus "Toivo Raidmets. Barokist dadani" Eesti Arhitektuurimuuseumis kuni 18. XII 2005. Loetletud valik T. Raidmetsa sise- ja näitusekujundusi, näitustel osalemised. 10 värv. ill

  15. Kasvatusstiilide rohkus ajab lapsevanemad segadusse / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2016-01-01

    Vestlusest Pärnumaa Rajaleidja keskuse psühholoogi Olga Šunjajevaga, kes uuris perekasvatuse rolli viie- kuni seitsmeaastaste lastega peredes, nüüdisaegseid peremudeleid ning millist abi vanemad lastega tegeledes vajavad. Uurimusest sündis Olga Šunjajeva magistritöö Tallinna ülikooli kasvatusteaduste instituudis. Ka vanemlusprogrammist "Imelised aastad" - koolieelikute vanematele mõeldud koolitustest

  16. Snow Show Lapimaal / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2003-01-01

    2004. a. Rovaniemis läbiviidavast rahvusvahelisest jää- ja lumearhitektuuri üritusest, kuraator Lance Fung. Steven Holli jääplokkidest kuup - projekti pilootehitis. Osalevate arhitektide ja kunstnike osaline loetelu

  17. Kungla rahva kuldne aeg / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2016-01-01

    1970ndal aastal Lomonossovi tänavale (praegune Gonsiori tänav) ehitatud Kungla hotellist. Arhitektid Ilmar Puumets, Herbert Rüütlane; sisearhitektid Valter Pormeister, Ingrid Pormeister, Maia Laul. Samale kohale ehitati hiljem 13-korruseline Hilton hotell

  18. Riik ja arhitektuur : prantsuse variant / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    1999-01-01

    Prantsuse arhitekti Dominique Perrault' (s. 1953) näitus Rotermanni soolalaos. Kuraator Eric Messerschnidt. Arhitektist, tema loomingust, pikemalt Prantsuse rahvusraamatukogu hoonest (1996; 1997. a. Mies van der Rohe' arhitektuuripreemia) ja Berlier' tootmishoonest (1990) Pariisis, Berliini velodroomist (1998). Franȯis Mitterrand'i arhitektuuripoliitikast

  19. Igavuse mõõt / Karin Bachmann

    Index Scriptorium Estoniae

    Bachmann, Karin, 1976-

    2011-01-01

    Tallinn astub samme elukeskkonna mitmekesisuse vähendamiseks. Üheks neist on muru pikkuse normeerimine. Lühidalt 2.-4.11.2011 Tallinnas toimuvast EFLA kongressist "Mind the Gap - Landscape for a New Era"

  20. Värske kunsti paraad / Karin Laansoo

    Index Scriptorium Estoniae

    Laansoo, Karin, 1976-

    2003-01-01

    30. V-8. VI Rotermanni soolalaos näitus "Eesti Kunstiakadeemia lõputööd 2003", mille on kujundanud Oliver Soomets ja Kaiko Kivi. Trükised on kujundanud Maya Zerebtsova. 6. VI moeetendus. Academia Grata lõpetajate teoseid esitletakse Pärnus, meediagraafika lõputöid kaitstakse 11. VI EKA aulas

  1. Wannabe - Ameerika ja Postkontor 2 / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2008-01-01

    Uued hotellid Tallinna kesklinnas: Kaksiktornid (arhitektuuribüroo Nord Projekt, Meeli Truu, sisekujundaja Meelis Press); Forumi hotell (arhitektuuribüroo Pluss, Indrek Allmann, sisekujundajad Kaire Kemp-Tishler ja Ea Andla)

  2. Piero Castiglioni - praktilise ilu looja / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2005-01-01

    Itaalia disaineritest Castiglioni'dest, pikemalt Piero Castiglionist (sünd. 1944), kes külastas 2005. aasta märtsis Eestit. Loetletud tema tähtsamad tööd. Kommenteerivad sisearhitekt Hannes Praks, sisearhitekt Juta Lember, valgusdisainer Tarmo Luisk ja EKA professor Toivo Raidmets

  3. Linn toetab kohalikke kultuuritegijaid / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2008-01-01

    Pärnu linnavalitsuse toetuste saajate seas ka MTÜ Pärnu Kontserdibüroo, MTÜ Pärnu David Oistrahhi Festival, RE Eesti Kontsert Pärnu Kontserdimaja, MTÜ Pärnu Rahvusvaheline Koorifestival, MTÜ Jazzkaare Sõprade Ühing ja MTÜ Suupilliklubi Piccolo

  4. USA raport hoiatab tuumaterroristide eest / Karin Volmer

    Index Scriptorium Estoniae

    Volmer, Karin

    2007-01-01

    USA-s tegutseva tuumaterrori vastase organisatsiooni Nuclear Threat Initiative (NTI) raport kinnitab maailma edusamme tuumamaterjali turvalisuses, kuid on ka palju ohuallikaid. Analüütikud kahtlevad Venemaa ja Pakistani armee usaldusväärsuses tuumamaterjali hoidmisel. Lisa: Tuumaterrori raport

  5. Tükk demokraatlikku arhitektuuri / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2008-01-01

    Taani firma Schmidt Hammer Lassen Architects koostab Tallinnasse Paljassaare poolsaarele kavandatava ECOBAY-linnaku planeeringut. Projekti kontseptsiooni kirjeldus. Taani projekteerimisfirma iseloomustus. Asutajad Morten Schmidt, Bjarne Hammer, John Lassen. Morten Holmi kommentaar

  6. Non vi sed arte / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2007-01-01

    Eesti Betooniühing valis 2006. aasta parimaks betoonehitiseks Tallinnas Tartu maantee ääres asuva Luku-Experdi büroohoone (arhitekt Hindrek Kesler, konstruktor Ago-Allan Kuddu, ehitaja Ahti Väin Konsult OÜ, betooni autor AS E-Betoonelement). Ehitaja eriauhinna pälvis "Eesti Ehitus" Muuga viadukti eest. Kommenteerib Aadu Kana

  7. Siivsalt lahendatud Skoone bastion / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2005-01-01

    Tallinna vanalinnas asuva Skoone bastioni ja sellega külgneva staadioni arendamiseks väljakuulutatud ideekonkursi võidutöödest. Peapreemia sai töö "Glassii" (autorid Maarja Kask, Ralf Lõoke, Karli Luik arhitektuuribüroost Salto). Teise preemia pälvis töö "SK-one" (autorid Timo Subbi, Mihkel Urmet, Taavi Kaldaru arhitektuuribüroost Suburb OÜ). Kolmanda preemia sai töö "Karl XI" (autorid Ott Kadarik, Villem Tomiste, Mihkel Tüür, Paco-Ernest Ulman arhitektuuribüroost Kosmos). Võistlustöödega saab tutvuda Arhitektuuri- ja Disainigaleriis. Harry Liivranna lühike ülevaade Skone bastioni ajaloost

  8. Rotermannid, sool ja soolaladu / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    2000-01-01

    Christian Barthold, Christian Abraham ja Christian Ernst August Rotermannist, nende ettevõtmistest. Rotermanni kvartali kujunemisest, Rotermanni Tehastest, soolalaost (projekt Ernst Boustedt; rekonstrueerimisprojekti arhitekt Ülo Peil büroost Urbel ja Peil, sisearhitekt Taso Mähar). 6 ill

  9. Kättpidi Veneetsias / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2006-01-01

    Muljeid X rahvusvaheliselt Veneetsia arhitektuuribiennaalilt "Linnad. Arhitektuur ja ühiskond", kuraator Richard Burdett. Ülar Margi kureeritud projektist "Joint Space", kommenteerib Rein Ahas. Väljavõtteid Rem Koolhaasi ja Jacques Herzogi esinemisest Arsenales. Andres Sevtshuki kureeritud projektist. Kuldlõvi auhinna pälvis inglise arhitekt Richard Rogers (sünd. 1933). Bernard Tschumi elliptilise linna projekti kommenteerib Kaja Pae

  10. Läti parimad majad / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2006-01-01

    Muljeid Läti arhitektuuriauhindade jagamiselt. Peapreemia: arhitektide Uldis Lukshevics'i ja Martins Oshans'i projekteeritud eramu. Parim avalik hoone: arhitektuuribüroo Kubs projekteeritud kontorihoone Riias. Suurim Baltikumis: arhitektuuribüroo Arhis projekteeritud Ljepaja politseihoone. Parim rekonstrueerimisprojekt: arhitektuuribüroo Arhis projekteeritud avatud kontor nõukogudeaegses tööstushoones. Parim interjöör: kohvik Vanilla (Ieva Dripe, Janis Broka) ja tekstiilipood Garage (Zaiga Gaile büroo). Uldis Lukshevics'i projekteeritud suvilad, mis paiknevad postidel

  11. Austraallased huvituvad ETV toodangust / Karin Leemet

    Index Scriptorium Estoniae

    Leemet, Karin

    2000-01-01

    ETV saadet "Simson Seakülast" näidatakse peagi Austraalia telekanalil The Special Broadcasting Service. 1995. a. valminud kunstisaade on eesti skulptorist Aivar Simsonist, saate autorid Mariina Mälk, Peeter Brambat, operaator Peep Laansalu. Mari Sobolev A. Simsoni töödest

  12. Deontiline modaalsus poliitikute arvamusartiklites / Karin Mandra

    Index Scriptorium Estoniae

    Mandra, Karin

    2008-01-01

    Lähtudes funktsionaalse grammatika põhimõtetest analüüsitakse, missuguste tegevuste ja protsesside vajalikkust poliitikud oma arvamusartiklites rõhutavad ning kuivõrd on esindatud deontiline aspekt (ehk kellele on direktiivsus suunatud)

  13. RIBA kuldmedal Oscar Niemeyerile / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    1998-01-01

    1998. a. RIBA (Royal Institute of British Architects) kuldmedal anti Rio de Janeiros üle brasiilia arhitektile Oscar Niemeyerile. Kuldmedali üleandmise pidulikust tseremooniast. Miks arvukate 20. saj. modernismi klassikasse kuuluvate hoonete autorile alles nüüd RIBA kuldmedal anti. Praegu 91 aastasel vanameistril valmis 1996. a. Rio de Janeiros Niter̤i Moodsa kunsti muuseum, ehitamist ootab Rio konverentsikeskus

  14. Kõikide mugavustega õnn massidele / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2004-01-01

    Kunstiteaduse Instituudi rahvusvaheline konverents "Võistlevad õnned. Elukeskkond külma sõja perioodil". Esines Adrian Forty, Hilde Heynen Hollandist, Julian Holder Shotimaalt, Kirsi Saarikangas Soomest

  15. Theodor Schweisfurth. Sozialistisches Völkerrecht? / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1981-01-01

    Tutvustus: Schweisfurth, Theodor. Sozialistisches Völkerrecht? : Darstellung - Analyse - Wertung der sowjetmarxistischen Theorie vom Völkerrecht "neuen Typs". Berlin [etc.] : Springer-Verlag, 1979. (Beiträge zum ausländischen öffentlichen Recht und Völkerrecht, Bd. 73)

  16. Kohalik kalandus ja kalandusteadus Euroopa perspektiivis : Pärnu lahe näide / Henn Ojaveer

    Index Scriptorium Estoniae

    Ojaveer, Henn, 1965-

    2009-01-01

    Uuringust, mis on teostatud EL kuuenda raamprogrammi vahenditest finantseeritud projekti "Teaduslik nõuanne kalavarude majandamiseks erinevatel tasanditel" (Scientific Advice for Fisheries Management at Multiple Scales, SAFMAMS)

  17. Sowjetunion und Völkerrecht 1962-1973 / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1980-01-01

    Tutvustus: Sowjetunion und Völkerrecht 1962-1973 : Bibliographie und analyse / hrsg. Boris Meissner, Dietrich Frenzke und Erika Chilicki. Köln : Wissenschaft und Politik, 1979 [t.p. incorrectly: 1977

  18. Rünnak Eesti põhiseadusliku korra vastu / Henn Põlluaas

    Index Scriptorium Estoniae

    Põlluaas, Henn

    2007-01-01

    Ilmunud ka: Pärnu Postimees, 9. mai 2007, lk. 18; Maaleht, 10. mai 2007, lk. 8; Õpetajate Leht, 25. mai 2007, lk. 5. Eesti demokraatlik-rahvuslike jõudude koostöökoja juhataja sõnul tuleks Eesti riikliku julgeoleku kaitsel ning vene ekstremistide tegevusele piiri panemisel ja nende karistamisel kasutada kõige karmimaid vahendeid, tuleb tühistada põhiseadusliku korra vastu üles astunud mittekodanikest isikute elamisload ja ebalojaalsete naturaliseeritud kodanike kodakondsus

  19. Encyclopedia of Public International Law, 7 / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1985-01-01

    Tutvustus.: Encyclopedia of Public International Law, published under the Auspices of the Max-Planck-Institute for Comparative Public Law and International Law under the Direction of Rudolf Bernhardt. (7. History of International Law. Foundations and Principles of International Law. Sources of International Law. Law of Treaties). Amsterdam, New York, Oxford: North-Holland Publishing Company, 1984

  20. Encyclopedia of Public International Law, 3 / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1983-01-01

    Tutvustus.: Encyclopedia of Public International Law, published under the Auspices of the Max-Planck-Institute for Comparative Public Law and International Law under the Direction of Rudolf Bernhardt. 3 (A_M) and 4 (N-Z): Use of Force. War and Neutrality. Peace treaties. Amsterdam, New York, Oxford: North-Holland Publishing Company, 1982

  1. Encyclopedia of Public International Law, 2 / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1982-01-01

    Tutvustus: Encyclopedia of Public International Law, published under the Auspices of the Max-Planck-Institute for Comparative Public Law and International Law under the Direction of Rudolf Bernhardt. (2. Decisions of International Courts and Tribunals and International Arbitrations). Amsterdam, New York, Oxford: North-Holland Publishing Company, 1981

  2. Encyclopedia of Public International Law, 5 / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1983-01-01

    Tutvustus: Encyclopedia of Public International Law, published under the Auspices of the Max-Planck-Institute for Comparative Public Law and International Law under the Direction of Rudolf Bernhardt. 5. International Organizations in General. Universal International Organisazations and Cooperation. Amsterdam, New York, Oxford: North-Holland Publishing Company, 1983

  3. Encyclopedia of Public International Law, 9 / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1988-01-01

    Tutvustus: Encyclopedia of Public International Law / published under the Auspices of the Max-Planck-Institute for Comparative Public Law and International Law under the Direction of Rudolf Bernhardt. (9. International Relations and Legal Cooperation in General. Diplomacy and Consular Relations and 10. States. Responsibility of Sates. International Law and Municipal Law). Amsterdam, New York, Oxford : North-Holland Publishing Company, 1986-1987. Pp. XV, 425 and pp. XV, 543

  4. Encyclopedia of Public International Law, 8 / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1986-01-01

    Tutvustus: Encyclopedia of Public International Law, published under the Auspices of the Max-Planck-Institute for Comparative Public Law and International Law under the Direction of Rudolf Bernhardt. (8. Human Rights and the Individual in International Law. International Economic Relations). Amsterdam, New York, Oxford: North-Holland Publishing Company, 1985

  5. Rudolf Streinz. Meinungs- und Informationsfreiheit zwischen Ost und West / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1983-01-01

    Tutvustus: Streinz, Rudolf. Meinungs- und Informationsfreiheit zwischen Ost und West : Möglichkeiten und Grenzen intersystemarer völkerrechtlicher Garantien in einem systemkonstituierenden Bereich. Ebelsbach : Rolf Gremer, 1981

  6. Tänavaruumi sõnastik = A glossaryof street space / Henn Runnel

    Index Scriptorium Estoniae

    Runnel, Henn, 1983-

    2012-01-01

    Tallinna linnainstallatsioonide festivali "LIFT11" installatsioonist "Tänavaruumi audiotuur", mis tutvustab Roosikrantsi tänava näitel ühte iseloomulikku arhitektuurset ruumi Tallinnas. Autor Kadri Klementi. Väljavõte "Tänavaruumi audiotuurist"

  7. Encyclopedia of Public International Law, 6 / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1984-01-01

    Tutvustus: Encyclopedia of Public International Law, published under the Auspices of the Max-Planck-Institute for Comparative Public Law and International Law under the Direction of Rudolf Bernhardt. (6. regional Cooperation, Organization Problems). Amsterdam, New York, Oxford: North-Holland Publishing Company, 1983

  8. Üks vormiõpetus (I) : Vaimumõõgad / Henn Saari

    Index Scriptorium Estoniae

    Saari, Henn, 1924-1999

    1998-01-01

    Diskussioonist eesti nüüdiskeele võrmiõpetuse ning selle eelduseks oleva fooniasüsteemi üle uut õigekeelsussõnaraamatut ette valmistades. Vt. ka: Ehala, Martin. Eesti morfoloogia olemus // Keel ja Kirjandus, 1997, nr. 6 ; Hint, Mati. Eesti foneetika möödunud hiilgus ja möödumata viletsus // Keel ja Kirjandus, 1997, nr. 2 ; Ross, Kristiina. Eesti morfo(fono)loogia põnevamad probleemid : EKG contra Mati Hint // Keel ja Kirjandus, 1997, nr. 5

  9. 10 aastat kostüümidraamat / Kaire Raave, Katrin Pauts, Kaja Roomets

    Index Scriptorium Estoniae

    Raave, Kaire

    2007-01-01

    Oma rõivastusest vabariigi aastapäeva presidendi vastuvõttudel räägivad Eesti Kunstiakadeemia rektor Signe Kivi, dirigent Eri Klasi abikaasa Ariel, Riigikogu liige Evelyn Sepp, tippärimehe Urmas Sõõrumaa kaasa Kätlin Maran ja Võru linnapea Ivi Eenmaa. Lisatud valik fotosid presidendi vastuvõttudest aastatel 1999-2006. Vt. samas: President Toomas Hendrik Ilves 9 aastat presidendi vastuvõtul. Pikemalt Signe Kivi kleitidest ja näitusest "24. veebruar" Disaini- ja Arhitektuurigaleriis, kus on eksponeeritud tema kõiki vastuvõttudel kantud kleite

  10. Veikko Täär : mees võib olla naise ori / Kaire Raave

    Index Scriptorium Estoniae

    Raave, Kaire

    2007-01-01

    Jaan Toominga lavastus "Venuse armumängud" austria kirjaniku Leopold von Sacher-Masochi romaani "Venus karusnahas" järgi. Osades Veikko Täär, Britta Vahur. Näitleja Veikko Täär endast ja oma rollist. Lisaks tutvustus "Venuse armumängud"

  11. Kolm inimest tuhandest kannab HI-viirust / Karin Kangro

    Index Scriptorium Estoniae

    Kangro, Karin

    2004-01-01

    Poliitikauuringute keskus Praxis uuris aidsiennetustegevust ja selle rahastamist. Tervishoiuspetsialist Maris Jesse sõnul tehakse Eestis aidsiennetustegevuses õigeid asju, ainult alarahastatult ja liiga vähe. Eiki Nestor, Nelli Kalikova aidsiprobleemist, ennetustööst. Vt. samas: Ka Euroopa Eesti pärast mures

  12. Juhtumikorralduse põhimõtte rakendamisest kohalikus omavalitsuses / Karin Hanga

    Index Scriptorium Estoniae

    Hanga, Karin

    2005-01-01

    27.-28. juunil 2005. a. Pedasel toimunud kohalike omavalitsuste sotsiaal- ja lastekaitsetöötajate suvepäevadel arutluse all olnud juhtumikorralduse probleemidest. Lisatud juhtumi lahendamise skeem. Kommenteerivad: Merle Linno ja Indrek Rohtla

  13. Retrovõtmes pühamu / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2005-01-01

    Tallinnasse Lasnamäele plaanitava Jumalaema kiriku arhitektuurikonkursi võidutööst arhitektuuribüroolt Z-Projekt (arhitektid Oleg Zhemtshugov, Nikolai Djatko, Jevgeni Kolomenkin). Kommenteerib kunstiteadlane Mari-Liis Paaver. Ergutuspreemiad said arhitektuuribüroo ZiZi & YoYo projekt (arhitektid Veronika Valk, Yoko Alender) ning arhitektuuribüroo Lokomotiiv (arhitektid Jüri Martson, Rasmus Reinolt, Ivo-Martin Veelma)

  14. 2008. aastal tõuseb pension 22% / Karin Kalda

    Index Scriptorium Estoniae

    Kalda, Karin

    2007-01-01

    Ilmunud ka: Tallinna Teataja : Kesklinn, Lasnamäe, Pirita, okt., 2007, lk. 3; Tallinna Teataja : Haabersti, Kristiine, Põhja-Tallinn, okt., 2007, lk. 3; Tallinnskii Vestnik, okt., 2007, lk. 3. Tallinnskii Vestnik autorita. Valitsusliit on saanud valmis uue pensioniarvestuse süsteemi, mille alusel võetakse aastahinde arvutamisel senisest enam arvesse sotsiaalmaksu laekumist. Kommenteerib Taavi Rõivas

  15. Mänguväljak rikastab linnakeskkonda / Karin Bachmann

    Index Scriptorium Estoniae

    Bachmann, Karin, 1976-

    2005-01-01

    Mänguväljakute rajamisest ja korrashoiust Tartus, kus on olemas ülelinnaline mänguväljakute teemaplaneering. Projekt algatati 2002. a., eestvedaja Angela Männiste. Arvamust avaldavad Andres Pool ja Indrek Ranniku Tartu linnavalitsusest. Tallinnal ülelinnaline plaan mänguväljakute rajamiseks puudub, olukorrast Mustamäel ja Nõmmel. 3 ill

  16. Euroliit vaidleb Birma diktatuuri üle / Karin Dean

    Index Scriptorium Estoniae

    Dean, Karin

    2004-01-01

    EL-i ja Aasia riikide diplomaatilisest vaidlusest, kas lubada EL-i ja Kagu-Aasia tippkohtumisele diktatuuririiki Birmat. Kagu-Aasia maade ühenduse ASEAN seisukohtadest. Vt. samas: Birma uue põhiseaduse arutelu põlu all

  17. Kapitalistlik realism muudab Vietnami majandustiigriks jalgrattal / Karin Dean

    Index Scriptorium Estoniae

    Dean, Karin

    2007-01-01

    Ilmunud ka: Postimees : na russkom jazõke 13. apr. lk. 10-11. Vietnami majanduspoliitikast, odavast tööjõust, edukatest reformidest ja majanduskasvust. Kaart: Vietnami Sotsialistlik Vabariik. Vt. samas: Sõda oli püüd takistada kommunismi

  18. Arhitektuuribiennaalil arhitektuurile mõeldes / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2008-01-01

    Veneetsia arhitektuuribiennaalist teemal "Out there : Architecture Beyond Buildings". Biennaali üldkuraator Aaron Betsky. Eestlaste gaasitoruinstallatsioonist (autorid Maarja Kask, Ralf Lõoke, Neeme Külm). Lühidalt Hispaania, Inglismaa, Prantsusmaa, Hollandi, Taani, Saksamaa, Hiina ja Poola ekspositsioonidest, pikemalt Venemaa paviljonist. Nigel Coatsi, Zaha Hadidi, Frank Gehry, Herzog & de Meuroni ja Ai Weiwei töödest biennaalil, filmist Rem Koolhaasi Bordeaux' villast. Vt. repliik: Ruudi, Ingrid. Hea Sirbi toimetus! (Sirp, 2009, 30. jaan., lk. 8)

  19. Gaasitoru, ujuv saun ning helisevad tuutud / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2008-01-01

    Veneetsia XI arhitektuuribiennaalile kandideerinud tööde tutvustus. Biennaali teemaks on "Majade taga. Arhitektuur väljaspool ehitisi". Eestit esindab projekt "Gaasitoru", autorid Maarja Kask ja Ralf Lõoke arhitektuuribüroost Salto ning skulptor Neeme Külm. Teise koha said Eve Kask, Signe Kivi ja Andrus Kõresaar tööga "Peatus". Kolmas koht läks Villem Tomiste projektile "Veealune Veneetsia"

  20. Iisraeli ja Palestiina suhted on paranemise teel / Karin Volmer

    Index Scriptorium Estoniae

    Volmer, Karin

    2007-01-01

    Iisraeli valitsus kiitis heaks palestiinlastest vangide vabastamise, keda peeti president Mahmud Abbasi liitlasteks võitluses Hamasiga. Iisraeli välisminister Tzipi Livni kohtus Palestiina peaministri Salam Fayyadiga. Vt. samas: Palestiinlaste sisetüli. Ilmunud ka: Postimees : na russkom jazõke, 10. juuli 2007, lk. 7

  1. Märgita tooted lahjendavad õlleturgu / Karin Vene

    Index Scriptorium Estoniae

    Vene, Karin, 1983-

    2008-01-01

    Kaubandusketid tellivad tooteid oma märgi all, nn. private label'eid, õlletootjate liidu andmeil on juba kümnendik kodumaisest õlleturust niisuguste toodete käes. Balti riikide toiduainesektori analüütiku Aavo Koppeli väitel sünnib seda vähem private label'eid, mida killustunum on kaubanduskettide turg. Graafikud: Kaubanduse õllebrändid Balti turul

  2. Püüd kaevuda vaataja alateadvusse / Karin Saarepuu

    Index Scriptorium Estoniae

    Saarepuu, Karin

    2002-01-01

    Lahatakse installatiivset keskkonnaprojekti "X arvab, et ta on Y, aga tegelikult on Z" Viljandis Kilpkonna galeriis, mis on Rotermanni soolalaos toimuva näituse nn. visiitkaart. Autoriteks on Marko Mäetamm ning Lucy Harrison ja Pete Nevin Suurbritanniast

  3. Mitte ainult jätistele : fassaadid kodututele / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    1999-01-01

    Festival 'Backdoor media'99' koondnimetusega 'Fassaadid kodututele' Tallinnas. Andres Kure heliinstallatsioon, performance Tammsaare monumendi juures (kuraator Hanno Soans), Peterburi kunstnike Natalja Jegorova ja Olga Jakimanskaja videod, Silja Saarepuu kerjamiskarbid, Raoul Kurvitza video, samizdat-ajakiri.

  4. Ühe maja lugu / Kalle Vellevoog ; interv. Karin Paulus

    Index Scriptorium Estoniae

    Vellevoog, Kalle, 1963-

    2008-01-01

    Näitusest Arhitektuuri- ja Disainigaleriis, kus eksponeeritakse Arne Maasiku fotosid Kalle Vellevoo ja ehitusarhitekt Margus Tamme (arhitektuuribüroo JVR) projekteeritud pansionaadihoonest Pärnus Seedri tänav 4. Näituse ja kataloogi kujundas Martin Pedanik

  5. Gazpromil tuleb rahast puudus kätte / Karin Volmer

    Index Scriptorium Estoniae

    Volmer, Karin

    2007-01-01

    Ilmunud ka: Postimees : na russkom jazõke 16. juuli, lk. 7.Venemaa president Vladimir Putin ja Prantsusmaa president Nicolas Sarkozy leppisid kokku, et Gazpromi partneriks seni suurimate veealuste maagaasivarude kaevandamisel Barentsi meres asuval Shtokmani leiukohal saab Prantsuse firma Total. Lisa: Kas teate? Vt. samas: Jürgen Tamme. Läänemere põhja plaanitava gaasijuhtme tulevik on ebamäärane

  6. Seewald - kaunis koht Kunstiakadeemiale / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2005-01-01

    Seewaldi haiglakompleksi ajaloost. Maa-ala planeerimise konkursist. Osalesid arhitektuuribürood Koot ja Koot, QP arhitektid, Lokomotiiv, Urban Mark ning Andres Põime büroo Studio 3. Konkursi võitis büroo Lokomotiv (Jüri Martson, Rasmus Reinolt, Ivo-Martin Veelma). Eripreemiaga märgiti ära arhitektuuribüroo Urban Mark (autor Ülar Mark) võistlustöö

  7. Linnade lömastavast hoost / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2004-01-01

    Arhitektuuriteadlase Andres Kure abiga koostatud linnatemaatikale keskendunud "Vikerkaare" erinumbrist (2004, 4-5). Michel de Certeau, Walter Benjamini, Jean-Paul Sartre, Boris Groysi, Jan Kausi ja Mart Kalmu artiklitest, Arne Maasiku fotodest jm.

  8. Noored - tööelanikkonna tulevik / Karin Reinhold

    Index Scriptorium Estoniae

    Reinhold, Karin, 1976-

    2003-01-01

    Espoos 20.-22. novembrini 2002. a. toimunud sümpoosionil "Noored ja töö" käsitleti kolme peateemat: õpilaste ettevalmistamine tööeluks, üleminek koolist tööle, noored töötajad ja tervislik tööelu

  9. Europa Nostra medal Tartusse / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2002-01-01

    Alvar Aalto projekteeritud Tammekannu villa (1932) Tartus pälvis Europa Nostra medali, mis antakse eriti õnnestunult renoveeritud objektile. Villa renoveeriti soome arhitekti Tapani Mustoneni projekti järgi

  10. Loodusteaduste olümpiaad tulekul / Karin Hellat

    Index Scriptorium Estoniae

    Hellat, Karin

    2006-01-01

    Euroopa Liidu kolmas noorte loodusteaduste võistkondlik olümpiaad toimus 2005. a. Iirimaal, 2005. a. detsembris toimus teistkordselt Indoneesias rahvusvaheline loodusteaduste olümpiaad. 2006. a. toimub Euroopa Liidu 4. loodusteaduste olümpiaad Brüsselis ning 3. rahvusvaheline loodusteaduste olümpiaad leiab aset Sao Paulos. Eesti loodusteaduste olümpiaadi voorud toimuvad veebruaris ja märtsis

  11. Eesti läheb Pekingisse / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2008-01-01

    Pekingi Eesti saatkonnahoone konkursist žüriiliikme pilguga. Premeeritud ja esiletõstetud töödest. Võidutöö autorid - Lauri Vaimel, Andres Põime, Liisa Põime; II koht - Erik Nobel ja Nobel Arkitekten, Taani-Eesti; III koht - Kalle Vellevoog, Velle Kadalipp, Tiiu Truus, Martin Prommik; ostudega esiletõstetud tööde autorid - Priit Pent ja Raul Järg Bucoma OÜ-st ning Jan Skolimowski, Peeter Loo, Kaspar Kruuse ja Li Vene KAMP Arhitektide OÜ-st. Hiina ja Pekingi uusehitistest, arhitektidest

  12. Juugend - rahvusliku arhitektuuri esimene importstiil / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2001-01-01

    12. V toimunud Eesti Arhitektuurimuuseumi rahvusvahelisest art nouveau konverentsist, mis keskendus Rootsi-Soome-Eesti ja Läti vastastikustele arhitektuurimõjutustele. Esinejatest, K. Hallase ettekandest sajandialguse arhitektuurisituatsioonist. Näitus "Riia juugendarhitektuur" Rotermanni soolalaos 20. maini.

  13. Hoovigalerii : uued hooned ja rajatised / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2004-01-01

    2003. a. korraldatud arhitektuurivõistlusest Eesti Panga hoonekompleksi erinevaid osi ühendava galerii lahenduse saamiseks. Panga juhatus palus esialgse eskiisi kallal edasi töötada arhitektuuribüroodel Schults ja Partnerid ning Urban Mark. Teostamiseks valiti Urban Marki töö. Hoovigalerii valmis 2004. a. 3 ill. konkursitöödest, 2 vaadet valminud galeriist

  14. Idast, ürgse väega / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2007-01-01

    Rändnäitus "Uue otsijad - praegune Ida-Euroopa!" Helsingi Arhitektuurimuuseumis. Eesti arhitektuuri esindavad Lootsi tänava kiilukujuline kortermaja (HG Arhitektuur), Villa V Nõmmel (3+1), Okupatsioonide muuseum (Head Arhitektid), Georg Otsa spaa (KOKO Arhitektid)

  15. Arhitektuurieurovisiooni võitjad selgunud / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    2009-01-01

    28. mail antakse Barcelonas üle 2009. a. Euroopa Liidu Mies van der Rohe nimelised kaasaegse arhitektuuri preemiad. Võitjaks tuli Oslo ooperi- ja balletiteater (arhitektuuribüroo Snohetta, arhitektid Kjetil Trædal Thorsen, Tarald Lundevall, Craig Dykers). Noore arhitekti eripreemia läks Horvaatia arhitektuuribüroole Studio Up (arhitektid Lea Pelivan, Toma Plejić) gümnaasiumi "46º09'N/16º50'E" eest Koprivnicas. Kataloogi valiti Eestist KOKO Arhitektide (Andrus Kõresaar, Raivo Kotov) projekteeritud Rotermanni kvartali laudsepatöökoja rekonstruktsioon

  16. SÄSI : kes on parim? / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2005-01-01

    Eesti parima noore disaineri auhinna SÄSI näitusest Eesti Kunstiakadeemia galeriis. Eksponeeritakse nominentide Anneli Tammiku, Monika Järgi, Riho Tiiveli, Sixten Heidmetsa, Igor Volkovi ja Björn Koopi töid

  17. Euroopa parim arhitektuur : kriteeriumidest ja valikutest / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2010-01-01

    Näitus "Euroopa parim arhitektuur. Euroopa Liidu Mies van der Rohe arhitektuuripreemiad 2009" Eesti Arhitektuurimuuseumis 5. septembrini 2010. Valiknäitusel on väljas Euroopas aastatel 2008-2009 ehitatud hooned. Preemia määrati arhitektuuribüroo Snøhetta Oslo ooperi- ja balletiteatrile. Noore büroona tõsteti esile Studio UP Horvaatiast. Eestist on näitusel KOKO Arhitektide laudsepa töökoda Rotermanni kvartalis

  18. Olmerti lahkumine külvab segadust / Karin Volmer

    Index Scriptorium Estoniae

    Volmer, Karin

    2008-01-01

    Iisraeli valitsusjuhi Ehud Olmerti tagasiastumisteade külvas kahtlusi Palestiinaga rahukõneluste jätkumise üle, kuid Jerusalem Posti andmeil üritab Olmert jõuda Palestiina liidri Mahmoud Abbasiga kokkuleppele veel enne uusi erakonnasiseseid valimisi

  19. Briti kultuuriprioriteedid - XX sajandi muuseumid / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2003-01-01

    2002. a. novembris Londonis toimunud Briti Nõukogu korraldatud seminarist "Managing Museums and Galleries of the 21st Century", mille rõhk oli kunstimuuseumidel. Inglise muuseumidest: Tate Modern, Baltic, British Museum, Victoria & Alberti muuseum, Londoni loodusmuuseumi Darwini keskus

  20. Disainiöös on asju / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2016-01-01

    Tallinna Disainiöö 12.-18. septembrini 2016. Uuest kodumaisest disainist (näiteid Marta Mooratsi, Tarmo Luisu, Veiko Liisi, Urmas Lüüsi, Ivar Kaasiku, Krista Leesi, Mariana Laane, Margot Holtsi, Jan Plaani, Pille Ernesaksa loomingust)

  1. Lasnamäe versus Tiskre / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2005-01-01

    Uuenevast Lasnamäest. Tulevasest Paekalda elurajoonist (arhitektuurikonkursi võitis Ilmar Klammer), Valge tänava elamutest (Muru & Pere), korterelamust Spectrum Liikuri tänaval (Künnapu & Padrik), Rohelise Kalda asumist (R-Konsult), kahest Ülo Peil büroo kavandatud 8-korruselisest elamust, Mäevärava majast (arhitekt Ilmar Klammer), Jumalaema kiriku projektist (Oleg Zhemtshugov, Nikolai Djatko, Jevgeni Kolomenkin). 9 värv. ill

  2. 3000 eesti peret rootsi kardinate taha / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    2000-01-01

    Jüri Mõisa ideest hoonestada Pikaliiva Rootsi kataloogimajadega Bo Klok. IKEA ja Skanska üürnike ühenduse majadest. Günther Behnischi projekteeritud, Ingolstadti ehitatavast sotsiaalmajade asumist. Olemasolevad odavate majade projektid Eestis. Arhitektuuripoliitikast

  3. Eesti saatkonna ehitamisest Riiga 1938. aastal / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2008-01-01

    Riiga Eesti saatkonna hoone rajamise katsest. Välisministeeriumi poolt Aleksander Nürnbergilt tellitud eskiisist. 1938. a. korraldatud arhitektuurikonkursist ja selle tulemustest (I preemia - Erich Jacoby ja Hermann Berg, II - Herbert Johanson ja Lorenz Johanson, 1939. aastast Lorenz Haljak, III - Elmar Lohk)

  4. Kino kui grafiti = Cinema as graffiti / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2001-01-01

    Kobarkinost Coca-Cola Plaza Tallinnas. Projekteerija: Arhitektuuristuudio Kirsima ja Niineväli. Arhitekt Andri Kirisma. Üldruumide sisekujundus: Kaire Kemp-Tisler, Ea Andla. II korruse baari kujundas Ursula Sõber. Konstruktsioonid: Marti Sein, Priit Luure Neoprojektist. Akustiline osa: Linda Madalik. Seinamaalingud: Anu Samarüütel. Projekt 1999-2001, valmis 2000-2001. 12 ill.: lõige, I ja V korruse plaanid, välis- ja sisevaated

  5. President Ilves jagas Ruila lastele aabitsaid / Karin Gustasson

    Index Scriptorium Estoniae

    Gustasson, Karin

    2008-01-01

    Kernu valla Ruila kooli 138. õppeaasta algas piduliku aktusega, kus tervituskõnega esines president Toomas Hendrik Ilves. President andis esimesse klassi astuvale 18 õpilasele isiklikult üle aabitsad pühendusega "Õpi rõõmuga!"

  6. Pärnut ootab kirev kultuuriaasta / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2007-01-01

    Pärnu linnavalitsuse kultuuriosakonna juhataja Jana Moosari sõnul 2007. aasta kultuuriürituste toetuste saajate hulgas on: MTÜ David Oistrahhi festival, MTÜ Pärnu Kontserdibüroo, muusikafestival "Muusikamoos", MTÜ XXI Sajandi Orkester (Klaudia Taevi nim. ooperilauljate konkursi korraldamine), MTÜ Suupilliklubi Piccolo, Pärnu kontserdimaja, MTÜ Arnold Scönbergi Ühing, MTÜ Jazzkaare Sõprade Ühing

  7. Kontserdimajas kõlab brasiilia muusika / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2009-01-01

    2. juulil Pärnu kontsedimaja kammersaalis toimuval kontserdil kavaga Musica Brasileira esineb Brasiilia duo Daniel Marques-Sergio Krakowski koos Eesti laulja-instrumentalisti Ele Raikiga, esinejatest

  8. Turistid-sõjaõhutajad : dessant Iisraeli / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    1999-01-01

    Kultuuridessandist Marika Valgu ja Helle Puusepaga Iisraeli. Jeruusalemma Yad Vashemist ئ Holocausti muuseumist. Arhitekt Moshe Safdie Laste Memoriaali ja Yad Vashemi memoriaali uue hoone lahendusest. Tel Avivi ja Jeruusalemma arhitektuurist ئ funktsionalismist, praegusest arhitektuurist, arhitekte. James Turrelli kunstiteosest "Ruum, mis näeb" Jeruusalemma muuseumi pargis

  9. Kompleksi kujunemine ja tsaariaegsed ehitised / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2004-01-01

    20. sajandi alguse Tallinna arhitektuurist. Pangakvartali kujunemise algus: Eestimaa Aadli Mõisate Krediitseltsi (Aadlipanga) hoone Estonia pst. 11 (1902-1904, arhitekt August Reinberg, ehituse järelevalve: Carl Jacoby, stukitöö, reljeefid: August Volz, vitraaž, vapid: Ernst Tode). Venemaa Riigipanga Tallinna osakonna hoone Estonia pst 13 (1907-1908, ehituse järelevalve: Aleksandr Jaron). Panga teenistujate elamu Sakala 6

  10. Käsitöö teeb asjad paremaks / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2007-01-01

    Eesti ja inglise kunstnike ühisnäitusest "Minu maailm : uus subjektiivsus kaasaegses disainis" Eesti Tarbekunsti- ja Disainimuuseumis. Kuraatorid Emily Campell, Andree Cook, Kai Lobjakas. Eksponeeritakse Annike Laigo, Krista Leesi, Ülle Jehe, Kärt Ojavee töid. Inglismaad esindavad 7 kunstnikku, lähemalt Danny Browni ja Alison Willoughby töödest

  11. WAF Dance etendab "Väikest printsi" / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2006-01-01

    Pärnu tantsukool WAF Dance toob 17. ja 18. juunil Endla teatris lavale Saint-Exupery "Väikese printsi". See on moodsa tantsuteatri koolitus- ja etendusprojekt. Lavastaja ja koreograaf on Kate Pringle Londonist

  12. Kunstiakadeemia lõpetajate defilee / Harry Liivrand, Karin Paulus

    Index Scriptorium Estoniae

    Liivrand, Harry, 1961-

    2007-01-01

    Tutvustatakse 2007. aastal Eesti Kunstiakadeemia lõpetanute töid: klaasikunstnik Kristi Ringkjobi klaasobjektide komplekt "Munk"; ehtekunstnik Raili Arbuse ehted; keraamik Ann Nurga savist "Lennukid"; klaasikunstnik Lauri Kimmi "Klaas ja kivi. Muundumine"; nahakunstnik Allan Põllu kollektsioon "Coffee and Cigars. Chocolate and Cognac"; sisearhitekt Ott Kanguri lamamis/kiiktool; arhitekt Rene Sauemägi visioon Admiraliteedi basseini äärsest tühermaast; Kristiina Uslari dekoratiivobjektid; arhitekt Sander Aasa projekt "Pakri saared. Kultuurmaastiku koloniseerimine"

  13. PAX6 MiniPromoters drive restricted expression from rAAV in the adult mouse retina

    Directory of Open Access Journals (Sweden)

    Jack W Hickmott

    2016-01-01

    Full Text Available Current gene therapies predominantly use small, strong, and readily available ubiquitous promoters. However, as the field matures, the availability of small, cell-specific promoters would be greatly beneficial. Here we design seven small promoters from the human paired box 6 (PAX6 gene and test them in the adult mouse retina using recombinant adeno-associated virus. We chose the retina due to previous successes in gene therapy for blindness, and the PAX6 gene since it is: well studied; known to be driven by discrete regulatory regions; expressed in therapeutically interesting retinal cell types; and mutated in the vision-loss disorder aniridia, which is in need of improved therapy. At the PAX6 locus, 31 regulatory regions were bioinformatically predicted, and nine regulatory regions were constructed into seven MiniPromoters. Driving Emerald GFP, these MiniPromoters were packaged into recombinant adeno-associated virus, and injected intravitreally into postnatal day 14 mice. Four MiniPromoters drove consistent retinal expression in the adult mouse, driving expression in combinations of cell-types that endogenously express Pax6: ganglion, amacrine, horizontal, and Müller glia. Two PAX6-MiniPromoters drive expression in three of the four cell types that express PAX6 in the adult mouse retina. Combined, they capture all four cell types, making them potential tools for research, and PAX6-gene therapy for aniridia.

  14. PAX6 MiniPromoters drive restricted expression from rAAV in the adult mouse retina.

    Science.gov (United States)

    Hickmott, Jack W; Chen, Chih-Yu; Arenillas, David J; Korecki, Andrea J; Lam, Siu Ling; Molday, Laurie L; Bonaguro, Russell J; Zhou, Michelle; Chou, Alice Y; Mathelier, Anthony; Boye, Sanford L; Hauswirth, William W; Molday, Robert S; Wasserman, Wyeth W; Simpson, Elizabeth M

    2016-01-01

    Current gene therapies predominantly use small, strong, and readily available ubiquitous promoters. However, as the field matures, the availability of small, cell-specific promoters would be greatly beneficial. Here we design seven small promoters from the human paired box 6 (PAX6) gene and test them in the adult mouse retina using recombinant adeno-associated virus. We chose the retina due to previous successes in gene therapy for blindness, and the PAX6 gene since it is: well studied; known to be driven by discrete regulatory regions; expressed in therapeutically interesting retinal cell types; and mutated in the vision-loss disorder aniridia, which is in need of improved therapy. At the PAX6 locus, 31 regulatory regions were bioinformatically predicted, and nine regulatory regions were constructed into seven MiniPromoters. Driving Emerald GFP, these MiniPromoters were packaged into recombinant adeno-associated virus, and injected intravitreally into postnatal day 14 mice. Four MiniPromoters drove consistent retinal expression in the adult mouse, driving expression in combinations of cell-types that endogenously express Pax6: ganglion, amacrine, horizontal, and Müller glia. Two PAX6-MiniPromoters drive expression in three of the four cell types that express PAX6 in the adult mouse retina. Combined, they capture all four cell types, making them potential tools for research, and PAX6-gene therapy for aniridia.

  15. Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust.

    Directory of Open Access Journals (Sweden)

    David Eduardo Torres

    Full Text Available Puccinia horiana Hennings, the causal agent of chrysanthemum white rust, is a worldwide quarantine organism and one of the most important fungal pathogens of Chrysanthemum × morifolium cultivars, which are used for cut flowers and as potted plants in commercial production regions of the world. It was previously reported to be controlled by Lecanicillium lecanii, Cladosporium sphaerospermum, C. uredinicola and Aphanocladium album, due to their antagonistic and hyperparasitic effects. We report novel antagonist species on Puccinia horiana. Fungi isolated from rust pustules in a commercial greenhouse from Villa Guerrero, México, were identified as Cladosporium cladosporioides and Cladosporium pseudocladosporioides based upon molecular analysis and morphological characters. The antagonism of C. cladosporioides and C. pseudocladosporioides on chrysanthemum white rust was studied using light and electron microscopy in vitro at the host/parasite interface. Cladosporium cladosporioides and C. pseudocladosporioides grew towards the white rust teliospores and colonized the sporogenous cells, but no direct penetration of teliospores was observed; however, the structure and cytoplasm of teliospores were altered. The two Cladosporium spp. were able to grow on media containing laminarin, but not when chitin was used as the sole carbon source; these results suggest that they are able to produce glucanases. Results from the study indicate that both Cladosporium species had potential as biological control agents of chrysanthemum white rust.

  16. Er fru Ruben jødisk eller ikke? En drøfting av hennes karakter i Hamsuns Siste Kapitel

    Directory of Open Access Journals (Sweden)

    Heini Mähönen

    2005-07-01

    utseende og andre klisjeer om jøder og han knytter disse opp til karakteren fru Ruben. Han konkluder med at hun må være jødisk, til tross for en slående mangel på konkret tekstlig belegg omkring karakterens religiøse og kulturelle tilknytning. På bakgrunn av Simpsons sterke påstand kombinert med mangelfull tekstlig illustrasjon, ønsker vi å drøfte fru Rubens jødiske opphav.

  17. "Rändur udumere kohal" ehk Ida-Viru maastikke otsides = Travellers above the mist - in search for the landscapes of Ida-Virumaa / Henn Runnel, Rait Parts

    Index Scriptorium Estoniae

    Runnel, Henn, 1983-

    2013-01-01

    Ida-Virumaa maastikest. Tööstusalade rekultiveerimisest ja taaskasutusprojektidest (põlevkivi hariduskeskus, Kiviõli seikluspark, Aidu sõudekanal jm.). Emscheri maastikupargist Saksamaal. Autorite sõnul võiks olemasolevate tööstusmaastike jätkuv kasutus ja nende hilisem rekultiveerimine käia piirkonna hilisemat potensiaali arvestavalt ja kujundavalt. Kaevanduste planeerimisel võiksid osaleda maastikuarhitektid

  18. The draft Geoblocking Regulation and its possible impact on B2C contracts / Karin Sein

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2017-01-01

    Euroopa Liidu määruse eelnõust, mis käsitleb siseturul asukohapõhist piiramist ja muul viisil diskrimineerimist kliendi kodakondsuse, elukoha või asukoha alusel ja selle eelnõu võimalikest tagajärgedest

  19. Pärnu võis poeedi elule lisada viis aastat / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2015-01-01

    Pärnu keskraamatukogus toimusid Tallinna Ülikooli korraldatud David Samoilovi 95. sünniaastapäeva tähistamise üritused - seminar-raamatuesitlus ning pärnaka Viktor Perelõgini fotonäitus. Esitleti Viktor Perelõgini raamatut "David Samoilov. Fotoalbum", Tallinna Ülikooli Humanitaarteaduste instituudi vene kirjanduse professori Irina Belobrovtseva koostatud luuleraamat "Mu sõbra avatud akna all. Jaan Kross ja David Samoilov" ning Tallinna Ülikooli Vene ja Ida-Euroopa uuringute suuna juhi Aurika Meimre koostatud teost "David Samoilovi Pärnu. Teejuht. Luuletusi"

  20. Polümeerne kultuuritegemine = Polymeric Culture-making / Karin Laansoo

    Index Scriptorium Estoniae

    Laansoo, Karin, 1976-

    2005-01-01

    Endises "Polümeeri" tehases Tallinnas 2002. aastast tegutsevast kultuuritehasest "Polymer". Hoones on töökojad, kunstistuudiod (Mare Raidma, Neeme Külm, Raul Keller), kultuuritrükikoda, tegutsevad klubid, toimuvad peod jm. 2005. a. juulist peab kultuuritehas pinnad vabastama, sest kinnisvarafirma Brem lõpetas 21. IV ühepoolselt rendilepingu

  1. Väike juveel Euroopa veere pääl / Thea Karin

    Index Scriptorium Estoniae

    Karin, Thea

    2012-01-01

    Küprose ajaloost, seal viibinud kuulsustest (Guido von Lusignan, Leonardo da Vinci, William Shakespeare, Lala Mustafa Paşa, Namik Kemal, Lawrence Durrell, Francis II Rakoczi) ning vaatamisväärsustest UNESCO pärandinimekirja kuuluvas Afroditele pühendatud Paphoses, pealinnas Nikosias, Girnes ja Famagustas, kus asuvad ka Shakespeare'i inspireerinud Othello torn ja Püha Nikolause katedraal, mis nüüd on Lala-Mustafa-Pasha mošee

  2. Eesti koondas Aasia uurijad ühise mütsi alla / Karin Dean

    Index Scriptorium Estoniae

    Dean, Karin

    2014-01-01

    Tallinna Ülikool, Tartu Ülikool ja Tallinna Tehnikaülikool lõid Aasia Uuringute Kompetentsikeskuse (AUKK), et koondada Aasia tänapäevase majanduse, poliitika ja ühiskondade teemal teadustööd tegevad teadlased Eesti ülikoolides ühte võrgustikku et pakkuda info- ja ideevahetust ülikoolide, avaliku sektori ja ettevõtjate vahel

  3. Demokraatia mitu elu / Karin Dean, Märt Läänemets

    Index Scriptorium Estoniae

    Dean, Karin

    2014-01-01

    Demokraatiast ühiskonnas Aasia ja Ida-Euroopa riikide näitel. Tallinna Ülikooli Eesti Humanitaarinstituudi korraldatud rahvusvahelisest konverentsist "Demokraatia mitu elu: Euroopast Aasia ja Vaikse ookeani regioonini"

  4. Hiina (ja Aasia) mõistmine pole lootusetu üritus / Karin Dean

    Index Scriptorium Estoniae

    Dean, Karin

    2011-01-01

    Eestlaste Hiina-huvi ja mõistmise soov on tingitud paljuski Hiina turust ja majanduslikest võimalustest. Mida on vaja teada, et Hiinat (Aasiat) mõista. Aasia-teemalise hariduse võimalustest Eestis

  5. Veeprojektid lähevad taas töösse / Karin Kroon

    Index Scriptorium Estoniae

    Kroon, Karin

    2007-01-01

    Ilmunud ka: Oma Saar, 17. okt. 2007, lk. 4; Meie Maa, 17. okt. 2007, lk. 4; Valgamaalane, 18. okt. 2007, lk. 2; Nädaline, 18. okt. 2007, lk. 4; Vooremaa, 18. okt. 2007, lk. 2; Võrumaa Teataja, 18. okt. 2007, lk. 2; Severnoje Poberezhje, 18. okt. 2007, lk. 2; Hiiu Leht, 19. okt. 2007, lk. 2; Põhjarannik, 19. okt. 2007, lk. 2. Keskkonnaministeeriumi veeosakonna projektide büroo juhataja esitab ministeeriumi lahendused EL Ühtekuuluvusfondi abiga veemajanduse korrastamisprojektide lõpuleviimiseks. Ajal. Oma Saar ka tabelid: 1. 2000-2006 projektide lisaraha jagumine; Hangete kallinemise katmine 2008. aastal

  6. Ago Anderson pälvis Helmi Tohvelmani preemia / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2009-01-01

    13. oktoobril anti Endla teatri näitlejale Ago Andersonile üle Helmi Tohvelmani auhind. Pidulik sündmus toimus Väätsa põhikoolis, Tohvelmani kodukohas. Anderson pälvis tunnustuse kui kerge kehakeelega näitleja

  7. Tallinna Planeerimine 1910. aastatel: rahvusvahelise planeerimiskonkursi ärgitajatest ja korraldajatest / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2014-01-01

    Uutest suundadest Tallinna linnavalitsemises 20. sajandi alguses. Tallinna Majaomanikkude Seltsist. Planeerimise asjatundjast varjunimega Vis. Kompetentsi küsimustest. Linnaplaani ettevalmistamise komisjonist.

  8. Maailm näkku : suurlinnalood Veneetsia X arhitektuuribiennaalilt / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2006-01-01

    Veneetsia X arhitektuuribiennaal "Cities. Architecture and Society". Richard Burdett'i kuraatorinäitus Arsenales. Vaatluse all on 16 maailmalinna: Tokyo, Mexico, New York, Sao Paulo, Mumbai, Shangai, Los Angeles, Kairo, Istanbul, London, Bogota, Barcelona, Milano-Torino, Berliin, Johannesburg, Caracas. Arvamusi biennaali kataloogist

  9. Laenusaaja õiguste kaitse SMS-laenu lepingute puhul / Kalev Saare, Karin Sein, Mari Ann Simovart

    Index Scriptorium Estoniae

    Saare, Kalev, 1974-

    2010-01-01

    SMS-laenu lepingutele kohaldatavad normid, laenuandja teavitamiskohustus ja tarbija võimalused lepingust taganeda. Laenuandja intressi-, viivise- ja leppetrahvinõuded, intressikokkuleppe lubatavuse piirangud

  10. Sõjavägi päästis poliitiku dzhunglist / Karin Volmer

    Index Scriptorium Estoniae

    Volmer, Karin

    2008-01-01

    Colombia sõdurid vabastasid sõjaväeoperatsiooni käigus Columbiat terroriseeriva armee FARC-i geriljade käest Prantsuse-Colombia poliitiku Ingrid Betancourti, kolm ameeriklast ja 11 kohalikku. Lisa: Colombia missioon

  11. Festivalipäevik Kanutist I / Margaret Tali, Riin Kübarsepp, Karin Laansoo...[jt.

    Index Scriptorium Estoniae

    2005-01-01

    NU Performance festivali "Continental Breakfast" Tallinn raames esinesid Kanuti Gildi saalis rootsi kunstnik Saralunden etendusega "Sweet Beat Tour", norra Baktruppen etendusega "DO & UNDO" ja soome kunstnik Tellervo Kalleinen etendusega "Let me"

  12. Pakistanis käib võitlus islamiriigi nimel / Karin Volmer

    Index Scriptorium Estoniae

    Volmer, Karin

    2007-01-01

    Pakistani pealinna Islamabadi Punase mošee ümber kestva, vähemalt 16 ohvrit nõudnud vastasseisu taga on islamiäärmuslaste soov kangutada ametist Ameerika-meelne president Pervez Musharraf. Vt. samas: Sündmused; Pakistan: kaart

  13. "Improvizz" toob Tartu Ülikooli aula lavale improvisatsiooniorkestri / Karin Palo

    Index Scriptorium Estoniae

    Palo, Karin

    2005-01-01

    Neljandast rahvusvahelisest improvisatsioonilise muusika festivalist "Improvizz" (festivali peakorraldajaks Aleksander Sünter). Kontserdid: "Opera Kit" 4. märtsil Tartu Sadamateatris, 5. märtsil Tartu Sadamateatris, 6. märtsil TÜ Ajaloomuuseumis, 6. märtsil "Ettearvamatu lõppkontsert" TÜ aulas

  14. Lähtugem ehitistest! / William J. R. Curtis ; interv. Andres Kurg, Karin Hallas, Triin Ojari

    Index Scriptorium Estoniae

    Curtis, William J. R.

    1998-01-01

    24. apr. pidas Rotermanni soolalaos loengu arhitektuuriajaloolane William J. R. Curtis. Katkeid jutuajamisest W. Curtisega. Arhitektuurikriitikast, oma raamatust "Modern Architecture since 1900", millest W. Curtis praegu kriitikuna kirjutab, Lille'i projektist jm

  15. Sotsiaalmaja Tallinnas Ankru 1 = Social Housing in Tallinn at 1 Ankru Street / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    2001-01-01

    Projekteerija Rein Murula Arhitektuuribüroo. Arhitekt Rein Murula, kaasa töötanud Martin Aunin, Margus Maiste, Karl Koosa. Konstruktsioonid: insener Tõnu Rausberg. Vanuritemaja I korrusel invakorterid. Projekt 1999, valmis 2000. 5 ill.: I korruse plaan, välisvaated

  16. On tegu ja nägu / Ralf Lõoke ; interv. Karin Paulus

    Index Scriptorium Estoniae

    Lõoke, Ralf, 1978-

    2008-01-01

    Tartu Kesklinna kool valmis 1912. aastal arhitekt Arved Eichhorni projekti järgi. 1965. aastal valmis Udo Tiirmaa projekti järgi juurdeehitis. 2007. aastal avati kooli laiendus, autoriteks arhitektuuribüroo Salto arhitektid Maarja Kask, Karli Luik ja Ralf Lõoke

  17. Tartust pärit eurolauljate võiduvõimalused vähenesid / Karin Palo

    Index Scriptorium Estoniae

    Palo, Karin

    2005-01-01

    Tänavusest Eesti Eurovisiooni finaalist, lauljast Laura Põldverest lauludega "Moonwalk ja "Let's Get Loud" (viimane ansambli Suntribe koosseisus)" ja Julia Bomani esitatud laulu "Nevermore Island" väljajätmisest finaalvõistlusest

  18. Abilinnapea Jaanson : kasvuruumi on küll / Karin Jaanson ; interv. Martin Shmutov

    Index Scriptorium Estoniae

    Jaanson, Karin

    2006-01-01

    Rahanduse eest vastutav Tartu abilinnapea vastab intervjuus 2006. aasta Tartu eelarvet puudutavatele küsimustele. Diagramm, tabel: Tartu linn kulutab tuleval aastal 1 360 535 800 krooni, investeeringud

  19. Vahemere ökosüsteemi ohustab haide vähesus / Karin Volmer

    Index Scriptorium Estoniae

    Volmer, Karin

    2008-01-01

    Vt. ka Postimees : na russkom jazõke 19. juuni, lk. 9. Rahvusvahelise Looduskaitseliidu (IUCN) raportist selgub, et haide arv Vahemeres on vähenenud viimase kahe sajandi jooksul 97 protsenti, seades sellega äärmiselt tõsisesse ohtu ökosüsteemi toimimise. Lisa: Vahemere haidJuuni viimasel päeval koos 28 kaaslannaga Mont Blancile ronival Anu Välbal alpinismikogemust pole

  20. Muuseumid ja mänedzhment : ICOM muuseumide maailmafoorumilt Barcelonas / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2001-01-01

    1.-6. VII toimunud ülemaailmse muuseumide organisatsiooni ICOM konverentsist üldteemal "Museums and Management". Muuseumidest informatsiooniajastul, USA muuseumide edukusest, muuseumide tulevikust (Ken Dychtwald, Rolf Jensen) ja juhtimisest.

  1. Ekspert : Pärnul tuleks jutustada oma lugu / Anu Jürisson, Karin Klaus

    Index Scriptorium Estoniae

    Jürisson, Anu, 1977-

    2009-01-01

    Rahvusvahelise kultuuriturismi konverentsist Pärnus ja kultuurianalüütik doktor Dragan Klaici soovitustest ning nägemusest omanäolise Pärnu kultuurielu elavdamiseks. Ants Johanson rääkis Viljandi folkmuusika festivali korraldamisest, külalised Tuula Rintala-Gardin ja Mikael Ahlerup ka teemaparkide loomisest

  2. Pangakontode arestimine pangakontode Euroopa arestimismääruse menetluses / Irina Pavlovskaja, Karin Sein

    Index Scriptorium Estoniae

    Pavlovskaja, Irina

    2014-01-01

    Euroopa Liidus 15. mail 2014 vastu võetud määrusest nr 655/2014, millega luuakse pangakontode Euroopa arestimismääruse menetlus, et hõlbustada võlgade piiriülest sissenõudmist tsiviil- ja kaubandusasjades. Artikkel põhineb autori 2014. a kevadel Tartu Ülikooli õigusteaduskonnas kaitstud magistritööl "Pangakonto arestimine kaitsemeetmena Euroopa Liidu tasandil Euroopa pangakonto arestimise määruse valguses"

  3. Piinatud birmalased ootavad ÜRO seni jõuetut abikätt / Karin Dean

    Index Scriptorium Estoniae

    Dean, Karin

    2007-01-01

    Buda munkade eestvedmisel toimunud massimeeleavalduste mahasurumisest Birmas. ÜRO erisaadiku Ibrahim Gambari kohtumisest nii Birma kindralite kui ka opositsiooniliidri Aung San Suu Kyiga oodatakse tulemusi ja sanktsioone Lisa: Sõjaväe haardes riik

  4. Arhitektuurimuuseum vaatab Aasiasse / Kim Sung Hong ; intervjueerinud Karin Hallas-Murula

    Index Scriptorium Estoniae

    Kim Sung Hong

    2009-01-01

    Näituse kuraatori Kim Sung Hongiga 21. aprillini Eesti Arhitektuurimuuseumis avatud Lõuna-Korea kaasaegse arhitektuuri näitusest "Megacity network" ("Megalinna võrgustik"), Korea arhitektuurist, arhitektidest, muinsuskaitsest ja avalikust ruumist. Näituse kujundas Hwang Doo Jin

  5. Pärnu jätkab Europan-projektiga / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2005-01-01

    2003. aastal toimunud arhitektuurikonkursi Europan 7 võidutöö "Teine loodus" (arhitektid Oana-Maria Bogdan, Kelly Shannon jt.) põhjal valminud eskiisprojektist Pärnus mere ning Tammsaare puiestee vahelisele krundile. Triin Ojari selgitab võistluse olemust. Arhitekt Kelly Shannon külastas Pärnut

  6. Mis toimub tudengiesinduste tagatoas pärast valimisi / Karin Pool

    Index Scriptorium Estoniae

    Pool, Karin

    2008-01-01

    Kohtumisest Tartu Ülikooli Üliõpilasesinduse teabespetsialisti Marju Himma, Tallinna Tehnikaülikooli üliõpilasesinduse juhatuse esimehe Oliver Kallase ja Tallinna Ülikooli üliõpilaskonna volikogu spiikri Rome Tikasega, kus arutati üliõpilasesinduste tegevust valimisjärgsel ajal

  7. Mercury Oxidation over Selective Catalytic Reduction (SCR) Catalysts - Ph.d. thesis Karin Madsen

    DEFF Research Database (Denmark)

    Madsen, Karin

    The vanadium-based SCR catalyst used for NOx-control promotes the oxidation of elemental mercury Hg0 to Hg2+ in flue gases from coal-fired power plants. Hg2+ is water soluble and can effectively be captured in a wet scrubber. This means that the combination of an SCR with a wet FGD can offer an e...

  8. Pärnu muuseumis näeb vammuseid ja pesu / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2009-01-01

    Näitus "Meie vammus" Pärnu Muuseumis, koostaja Ülli Kont. Viljandi Muuseumi rändnäitus "Need" Pärnu Muuseumis, koostajad Tiina Jürgen, Ebe-Triin Arros, Lüüli Kiik, Herki Helves. Näitused on avatud 12. septembrini

  9. Euroopa ühinev arhitektuuripoliitika : lõppes Euroopa esimene arhitektuurifestival / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2004-01-01

    Euroopa esimese arhitektuurifestivali üritustest. GAUDI (Governance, Architecture and Urbanism as Democratic Interaction) projektist. 2005. aastaks planeeritavasse GAUDI 2 projekti on Euroopa uute liikmesmaade arhitektuuriinstitutsioonide seas kutsutud Eesti Arhitektuurimuuseum. Arhitektuuri välisnäitustest Eestis, eesti arhitektuuripoliitikast, 2005. a. toimuva arhitektuurinädala korraldamisest

  10. Sigrid Viir võitis PULSE Kaasaegse Kunsti messi auhinna / Karin Laansoo ; fotod: Sigrid Viir

    Index Scriptorium Estoniae

    Laansoo, Karin, 1976-

    2012-01-01

    Laupäeval, 5. mail kuulutas PULSE Kaasaegse Kunsti messi preemiazhürii New Yorgis välja tänavuse messi peapreemia võitja - Eesti fotograafi Sigrid Viiru, keda esindas messil Temnikova & Kasela Galerii

  11. Uue aja moodne kodu ja ajaloo ringkäik / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2001-01-01

    1927. a. Stuttgarti ehitatud Weissenhofi väikemajade elamurajoonist (Mies van der Rohe, Le Corbusier, W. Gropius, M. Taut jt.) näituse "Weissenhof" põhjal Rotermanni soolalaos. Võrreldakse Emil Urbeli ja Indrek Ermi Aaviku küla projektiga Tiskres

  12. Paradigma muutus linnaplaneerimisideoloogias : nõukogulikust heaolumüüdist turumajandusliku diktaadini / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    1999-01-01

    Kirjutis põhineb Malle Meelaku mälestusele pühendatud linnaplaneerimisalasel konverentsil Tallinna Tehnikaülikoolis 27. mail peetud ettekandel, mille eesmärk oli vaadelda eesti XX sajandi teise poole linnaplaneerimisideoloogiat muu maailma mõjusuundade kontekstis. Tallinna uuest üldplaanist, pikemalt Viru väljakust.

  13. Millest räägivad paigad? : Paikade identiteet ja sotsiaalsed konstruktsioonid Eesti saartel / Karin Lindroos

    Index Scriptorium Estoniae

    Lindroos, Karin, 1973-

    2009-01-01

    Eesti suursaarte sotsiaalsete suhete struktuurist ja selle abil kujunevast paiga identiteedist, nende kindlaks tegemiseks ja kaardistamiseks sobilikust metoodikast ning paiga identiteedi kontseptsioonidest Hollandi geograafide Huigeni ja Meijringi kontseptsioonidele toetudes.

  14. Üleilmastumisel on linna nägu / Edward W. Soja ; interv. Karin Paulus

    Index Scriptorium Estoniae

    Soja, Edward W.

    2008-01-01

    Ameerika linnageograaf Edward W. Soja räägib globaliseerumisest, linnastumisest, rikkuse ebaühtlasest jaotumisest, subkultuuridest, inimese identiteedi muutumisest, raamatust "Thirdspace". Lühidalt Edward W. Soja tegevusest, foto. Lisa: Kes on Edward W. Soja?

  15. Oscar Niemeyer - arhitekt ja kõiksus / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2001-01-01

    Brasiilia arhitektist Oscar Niemeyerist (s. 1907) ja tema loomingust. Niemeyeri Rio de Janeiro büroo ja Rootsi Arhitektuurimuuseumi koostöös valminud näitusel Rotermanni soolalaos on rõhuasetus idee sünnil, esimestel skitsidel. Näitust saadab film Niemeyerist

  16. Kunstiakadeemia lõpetajad 2005 / Karin Paulus, Tanel Veenre, Tõnu Kaalep

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2005-01-01

    Valik Eesti Kunstiakadeemia bakalaureusetöid: Elize Hiiobi "Nimeta ehe", Anton Kooviti uued kirjatüübid, Raili Ilmsalu kipsskulptuur "Eva", Aleksei Aljoshini "Futuristlik spordirõivas", Merle Kasoneni töö "Võimalused", Leena Lüteri draakon "Isiklik kaitsja", Ave Maseri miniatuurid "Teised", Kärt Põldmani "Punased kingad", Riho Tiiveli vetelpäästesüsteem, Ester Kannelmäe rõivakollektsioon "Ahistab", töö "Eesti rahvalaulud", Helena Vaidla mosaiik "Veesilm", Merike Estna kineetiline maal "Punamütsike: I am the Prettiest Girl in the World", Eilve Mangluse käeobjektid, Annike Oldre maal "Üksi", Eliisa Ehini töö "Närviimpulsside liikumine ajus", Veiko Vaine terrassitool "Miinimum"

  17. Juhtumipõhise võrgustikutöö käsiraamat - abiks praktikutele / Karin Kiis

    Index Scriptorium Estoniae

    Kiis, Karin

    2006-01-01

    TÜ Pärnu Kolledzhi õppejõud töötasid välja käsiraamatu, mis põhineb juhtumipõhise võrgustikutöö koolitusel ning on mõeldud eelkõige sotsiaal-, rehabilitatsiooni-, kriminaalhoolduse- ning tööturuvaldkonnas töötavatele praktikutele

  18. Uue kunsti muuseum näitab loodus- ja lastefilmi / Karin Klaus

    Index Scriptorium Estoniae

    Klaus, Karin

    2009-01-01

    Tutvustatakse Pärnu uue kunsti muuseumis linastuvat ameeriklase Robert Harding Pittmani keskkonnateemalist dokumentaalfilmi "Tamm nr. 41940" ja Soome režissööri Kaisa Rastimo koguperefilmi "Torm" (Myrsky), 2008

  19. Eesti talendid raadiokonkurssidel "New Talent" ja "Let the Peoples Sing" / Karin Kopra

    Index Scriptorium Estoniae

    Kopra, Karin

    2005-01-01

    Bratislavas toimunud noorte interpreetide võistlusest "New Talent", kus osales ka pianist Mihkel Poll ning rahvusvahelisest amatöörkooride konkursist "Let the Peoples Sing" Kölnis, kus osalesid Eesti Televisiooni tütarlastekoor ja Tallinna Muusikakeskkooli lastekoor

  20. The influence of rAAV2-mediated SOX2 delivery into neonatal and adult human RPE cells; a comparative study.

    Science.gov (United States)

    Ezati, Razie; Etemadzadeh, Azadeh; Soheili, Zahra-Soheila; Samiei, Shahram; Ranaei Pirmardan, Ehsan; Davari, Malihe; Najafabadi, Hoda Shams

    2018-02-01

    Cell replacement is a promising therapy for degenerative diseases like age-related macular degeneration (AMD). Since the human retina lacks regeneration capacity, much attention has been directed toward persuading for cells that can differentiate into retinal neurons. In this report, we have investigated reprogramming of the human RPE cells and concerned the effect of donor age on the cellular fate as a critical determinant in reprogramming competence. We evaluated the effect of SOX2 over-expression in human neonatal and adult RPE cells in cultures. The coding region of human SOX2 gene was cloned into adeno-associated virus (AAV2) and primary culture of human neonatal/adult RPE cells were infected by recombinant virus. De-differentiation of RPE to neural/retinal progenitor cells was investigated by quantitative real-time PCR and ICC for neural/retinal progenitor cells' markers. Gene expression analysis showed 80-fold and 12-fold over-expression for SOX2 gene in infected neonatal and adult hRPE cells, respectively. The fold of increase for Nestin in neonatal and adult hRPE cells was 3.8-fold and 2.5-fold, respectively. PAX6 expression was increased threefold and 2.5-fold in neonatal/adult treated cultures. Howbeit, we could not detect rhodopsin, and CHX10 expression in neonatal hRPE cultures and expression of rhodopsin in adult hRPE cells. Results showed SOX2 induced human neonatal/adult RPE cells to de-differentiate toward retinal progenitor cells. However, the increased number of PAX6, CHX10, Thy1, and rhodopsin positive cells in adult hRPE treated cultures clearly indicated the considerable generation of neuro-retinal terminally differentiated cells. © 2017 Wiley Periodicals, Inc.

  1. Microglia acquire distinct activation profiles depending on the degree of alpha-synuclein neuropathology in a rAAV based model of Parkinson's disease.

    Directory of Open Access Journals (Sweden)

    Vanesa Sanchez-Guajardo

    Full Text Available Post-mortem analysis of brains from Parkinson's disease (PD patients strongly supports microglia activation and adaptive immunity as factors contributing to disease progression. Such responses may be triggered by alpha-synuclein (alpha-syn, which is known to be the main constituent of the aggregated proteins found in Lewy bodies in the brains of PD patients. To investigate this we used a recombinant viral vector to express human alpha-syn in rat midbrain at levels that induced neuronal pathology either in the absence or the presence of dopaminergic cell death, thereby mimicking early or late stages of the disease. Microglia activation was assessed by stereological quantification of Mac1+ cells, as well as the expression patterns of CD68 and MCH II. In our study, when alpha-syn induced neuronal pathology but not cell death, a fast transient increase in microglia cell numbers resulted in the long-term induction of MHC II+ microglia, denoting antigen-presenting ability. On the other hand, when alpha-syn induced both neuronal pathology and cell death, there was a delayed increase in microglia cell numbers, which correlated with long-lasting CD68 expression and a morphology reminiscent of peripheral macrophages. In addition T-lymphocyte infiltration, as judged by the presence of CD4+ and CD8+ cells, showed distinct kinetics depending on the degree of neurodegeneration, and was significantly higher when cell death occurred. We have thus for the first time shown that the microglial response differs depending on whether alpha-syn expression results on cell death or not, suggesting that microglia may play different roles during disease progression. Furthermore, our data suggest that the microglial response is modulated by early events related to alpha-syn expression in substantia nigra and persists at the long term.

  2. Valitsemine, riskijuhtimine ja õigusnormide järgimine kui tervik süsteem / Karin Rätsep

    Index Scriptorium Estoniae

    Rätsep, Karin

    2010-01-01

    Ajakirja The Economist poolt 2009. aasta septembris läbiviidud üleilmse uuringu valitsemise, riskijuhtimise ja õigusnormide järgimise terviksüsteemidele hinnangu andmiseks. Uuringu olulisemad järeldused. KPMG poolt väljatöötatud valitsemise, riskijuhtimise ja õigusnormide järgimise raamistikust. Skeem

  3. KARIN: The Ka-Band Radar Interferometer for the Proposed Surface Water and Ocean Topography (SWOT) Mission

    Science.gov (United States)

    Esteban-Fernandez, Daniel; Peral, Eva; McWatters, Dalia; Pollard, Brian; Rodriguez, Ernesto; Hughes, Richard

    2013-01-01

    Over the last two decades, several nadir profiling radar altimeters have provided our first global look at the ocean basin-scale circulation and the ocean mesoscale at wavelengths longer than 100 km. Due to sampling limitations, nadir altimetry is unable to resolve the small wavelength ocean mesoscale and sub-mesoscale that are responsible for the vertical mixing of ocean heat and gases and the dissipation of kinetic energy from large to small scales. The proposed Surface Water and Ocean Topography (SWOT) mission would be a partnership between NASA, CNES (Centre National d'Etudes Spaciales) and the Canadian Space Agency, and would have as one of its main goals the measurement of ocean topography with kilometer-scale spatial resolution and centimeter scale accuracy. In this paper, we provide an overview of all ocean error sources that would contribute to the SWOT mission.

  4. Pankrotis riik ja valusad reformid kärbivad Sarkozy senist tähelendu / Triin Oppi, Karin Volmer

    Index Scriptorium Estoniae

    Oppi, Triin

    2007-01-01

    Ilmunud ka: Postimees : na russkom jazõke, 25. sept. 2007, lk. 7. Postimees : na russkom jazõke lüh. Prantsusmaa presidendi Nicolas Sarkozy plaanib riigi pankrotist väljatoomiseks mitmeid seadusemuudatusi ja uuendusi. Peaminister Francois Fillon tunnistas eelarvepuudujääki. Lisa: Toetus. Väljavõtteid ajakirjale Time antud hinnangutest Nicolas Sarkozy valitsusajale: Philip H. Gordon, Marielle de Sarnez, Dominique Moisi

  5. Koolirõõmuta kool on lapsele kui sunnitöö / Ene-Silvia Sarv ; intervjueerinud Karin Klaus

    Index Scriptorium Estoniae

    Sarv, Ene-Silvia, 1945-

    2013-01-01

    Intervjuu Pärnus hariduskonverentsil "Õnnelik õpilane, õnnelik õpetaja" esinenud Tallinna ülikooli lektori Ene-Silvia Sarvega, kes rääkis oma ettekandes, milline paistab tänapäeva kool õpilaste silme läbi ja mis mõjutab koolikliimat kõige enam

  6. Büroohoone : Roopa 23/Tehnika 55, Tallinn = Office Building : Roopa 23/Tehnika 55, Tallinn / Karin Hallas

    Index Scriptorium Estoniae

    Hallas, Karin, 1957-

    2001-01-01

    Kolme-, osalt neljakorruseline katuseterrassiga büroohoone, mille silikaattellistest välisvoodriga fassaade ilmestab täiendavate materjalide (vaskplekk, puitlaudis) kombineerimine. Projekteerija: Rein Murula Arhitektuuribüroo. 9 ill.: tüüpkorruse plaan, lõige, välis- ja sisevaated

  7. Ring-wall enclosure of Lipa in Prehistoric Southern Harjumaa / Marge Konsa, Valter Lang, Karin Vimberg...[jt.

    Index Scriptorium Estoniae

    2006-01-01

    Lipa vallsulendik kuulub Eestis väikesearvuliste muististe hulka, mille iseloomulikuks tunuseks on tasasele maale ehitatud kividest või mullast madal vall, mis ringikujuliselt piirab õueala. Leitud keraamika järgi võiks asulakiht kuuluda ajavahemikku eelrooma rauaaja lõpust kuni rooma rauaaja lõpuni

  8. Opportunities and challenges in managing the process of knowledge sharing in different learning environments / Tiit Elenurm, Ly Hõbe, Karin Liikane

    Index Scriptorium Estoniae

    Elenurm, Tiit, 1952-

    2003-01-01

    Kaanel ajak. ilmumisaeg 2002/2003. Ülevaade kaugkoolituse (sh. veebipõhise õppe) kui teadmusjuhtimise meetodi võimalustest, puudustest, kuludest ning efektiivsusest teadmiste omandamisel ja rakendamisel praktikasse, toetudes EBS-i teadmusjuhtimise kursuste ja Eesti Pangaliidu kaugõppeprogrammide kogemustele. Tabelid

  9. Konstantin Päts ja Tallinna väljaehitamine pealinnaks 1934-1940 / Karin Hallas-Murula

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2015-01-01

    Uue riikliku ehituspoliitika kujunemisest 1934. aastal. Muudatustest linnaplaanis. Pealinliku fassadi ehitamisest. Ruumilise mõjuga projektidest. Narva maanteest ja Vabaduse väljakust. Roman Tavasti maja juhtumist

  10. Vintage: suvaline eriline vastikult eklektiline rohujuure disain = Vintage: arbitrary and peculiar, obnoxiously eclectic grassroots design / Karin Paulus

    Index Scriptorium Estoniae

    Paulus, Karin, 1975-

    2013-01-01

    Sisearhitekt Priit Põldme vintage stiilist. Armastusväärse kujundusega kohvikutest Eestis. Pikemalt kohvikust "Boheem" (Kopli t. 18, sisearhitekt Maris Kerge), "Kukeke" (Telliskivi t.), baarist "Pudel" (Telliskivi loomelinnak, sisekujundus: Pink) ja "Tops" (Soo t.) Tallinnas

  11. Töötajate varaline vastutus : [bakalaureusetöö] / Karin Lehari ; Tartu Ülikool, õigusteaduskond ; juhendaja: Merle Muda

    Index Scriptorium Estoniae

    Lehari, Karin

    1999-01-01

    Kahju tekitamisest tulenev kahju hüvitamine, töötaja poolt tekitatud kahju hüvitamise tingimused - varalise kahju olemasolu, töötaja teo õigusvastasus, põhjuslik seos, süü ja süü vormid, tööandja vastutus töötaja poolt kolmandatele isikutele tekitatud kahju eest

  12. Birma sõjavägi jätkab protestide mahasurumist toore jõuga / Karin Dean

    Index Scriptorium Estoniae

    Dean, Karin

    2007-01-01

    Sõjaväe ja meeleavaldajate kokkupõrgetest Birmas, armee rünnakutest kloostritele ja protestijatele, mille tulemusel on hukkunuid. ÜRO ja EL-i hukkamõistvast suhtumisest. Lisa: Sõjaväeline Birma

  13. Tehingu vorminõuded ja nende järgimata jätmise tagajärjed / Karin Sein

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2010-01-01

    Tehingu vorminõuete uuendustest 2002. aasta 1. juulist kehtivas tsiviilseadustiku üldosa seaduses. Vorminõude ulatusest ja funktsioonidest. Vorminõude järgimata jätmise tagajärgedest vastavate Riigikohtu lahendite põhjal

  14. ESTO ühendab kõiki, kes tunnevad endid eestlastena / Kristel Sarrik ; fotod: Vaado Sarapuu; Kristel Sarrik; Karin Rumming

    Index Scriptorium Estoniae

    Sarrik, Kristel

    2009-01-01

    Jaak Juhansoo ja Mart Laanemäe kõnedest, dokumentaalfilmist "Peod täis pisaraid", Kristjan Randalu ja Bodek Janke kontserdist, näitustest, sõjaveteranide kokkutulekust, kirikukonverentsist, kontserdist "Generatsioonid" jpm.

  15. Kükitajate kunst ja kõrvalhoidmise strateegiad = Squat-art and strategies of choosing the edge / Karin Laansoo

    Index Scriptorium Estoniae

    Laansoo, Karin, 1976-

    2002-01-01

    Prantsuse kunstnikud Delphine Reist, Laurent Faulon, Sebastien Perroud ja Mathiue Werchowsky tegid Tallinnas Kalinini tehasehoones squart'näituse "Neli laulu Siivile". Kuraator Andres Lõo.Four songs for Siivi, kunstinäitus (2002 : Tallinn)

  16. Avastatud maa-alune järv võib lõpetada Darfuri kodusõja / Karin Volmer

    Index Scriptorium Estoniae

    Volmer, Karin

    2007-01-01

    Ilmunud ka: Postimees : na russkom jazõke, 23. juuli 2007, lk. 6. Teadlaste hinnangul võib Darfuri konflikti lõpetada Darfuris avastatud maa-alune järv, sest kodusõda araablastest karjakasvatajate ja põlluharijatest põliselanike vahel võib tingitud olla veepuudusest. Kaart: Darfuri maa-alune järv. Lisa: Darfuri konflikt

  17. Recht und Gewalt im internationalen System. Von G. I. Tunkin. (Veröffentlichungen des Instituts für Internationales Recht an der Universität Kiel, Band 93) / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1989-01-01

    Raamatuarvustus: Tunkin, G. I. Recht und Gewalt im internationalen System. - Berlin : Duncker und Humblot, 1986. - (Veröffentlichungen des Instituts für Internationales Recht an der Universität Kiel ; 93). 213 lk.

  18. From Camps to Conversations in Critical Security Studies

    DEFF Research Database (Denmark)

    Hansen, Lene

    2008-01-01

    The article reviews the book "Critical Approaches to International Security," by Karin M. Fierke......The article reviews the book "Critical Approaches to International Security," by Karin M. Fierke...

  19. Inimesed ostavad kinnisvara asemel üha rohkem teemante / Kerli Nõu

    Index Scriptorium Estoniae

    Nõu, Kerli

    2005-01-01

    Eesti gemmoloogid Karin Eensaar ja Heli Kuulmann teemantidest kui investeeringust. Kommenteerib Karin Eensaar. Lisad: Maapõu peidab ülivanu kivikesi. Kultusehe number kaks: pärlikee; De Beers. Alrosa. BHP Billiton, Rio Tinto

  20. Reisirongiliiklus Võrumaal võib alata kõige varem nelja aasta pärast / Mari-Anne Leht

    Index Scriptorium Estoniae

    Leht, Mari-Anne, 1950-

    2004-01-01

    Reisirongiliikluse tulevikuvõimalusi Võrumaal analüüsivad Antsla vallavanem Tiit Tõnts, Võru maavalitsuse transpordi ja infrastruktuuri peaspetsialist Ahti Siimon ja Edelaraudtee juhatuse liige Henn Ruubel

  1. Lennureisija õigus saada hüvitist : Euroopa Liidu lennureisijate õiguste määrus / Karin Sein, Age Värv

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2013-01-01

    Euroopa Parlamendi ja nõukogu määrusest nr 261/2004. Reisijate õigusest rahalisele hüvitisele, lennuettevõtja hüvitise maksmise kohustusest ning kohtusse pöördumisest hüvitise nõude esitamiseks

  2. Bänd või korporatsioon? Identiteedivalikud kunstnike eneseorganiseerumisel = Band or corporation? Identity choises in the self-organisation of artists / Karin Laansoo

    Index Scriptorium Estoniae

    Laansoo, Karin, 1976-

    2009-01-01

    Aastatuhandevahetuse ümber Eestis loodud kunstirühmitustest, nende tekkimise põhjustest ja toimimisloogikast. Sotsialismiaegsed kollektivismi ideaalid kõrvutasid end uue, kapitalistliku bränditeadlikkusega. Pikemalt rühmitustest Troubleproduction, Avangard, Multikultuurimaja

  3. Töö vastuvõtmine ja töövõtja vastutus ehitusvigade eest / Piia Kalamees, Kalev Saare, Karin Sein

    Index Scriptorium Estoniae

    Kalamees, Piia, 1983-

    2010-01-01

    Töö vastuvõtmise kohustusest, töö valmimise ja vastuvõtmise õiguslikust tähendusest ja mõjust, töövõtja vastutuse ulatusest ja vastutusmudeli erisustest, ehitustöö valmiduse määratlemisest. Eesti ja Saksamaa seaduste ja kohtupraktika võrdlev analüüs

  4. Tähed disainiöös / Mait Summatavet, Risto Räägel, Per Mollerup...[jt.] ; interv. Karin Paulus

    Index Scriptorium Estoniae

    2007-01-01

    Disainiöö raames avati Arhitektuuri- ja Disainigaleriis näitus "Kokkupandavad asjad" ning kuulutati välja konkursi võitjad. Peapreemia võitis läti disaineri Elina Bushmane kokkupandav teelaud, teise koha Jaana Päeva tekstiilist kott, kolmanda koha Ingrem Floreni käekott. Ära märgiti Veiko Liisi kilepiima vutlar. Disainiöö üritustest ning konkursitöödest räägivad Mait Summatavet, Per Mollerup, Simo Heikkilä, Risto Räägel

  5. Tarbijalemüügilepingu sätete kohaldatavus tarbijast liisinguvõtja ja müüja vahelises suhtes / Karin Sein

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2012-01-01

    Liisinguvõtja õigustest liisingueseme puuduste korral ning tarbijalemüügi imperatiivsete sätete kohaldamisest. Võlaõigusseaduse § 365 lõige 1 Euroopa Liidu tarbijalemüügidirektiivi (1999/44/EÜ) kontekstis

  6. Karine Salomé, Les Îles bretonnes. Une image en construction (1750-1914), Rennes, Presses universitaires de Rennes, 472 p.

    OpenAIRE

    Guigueno, Vincent

    2005-01-01

    Des îles sans histoire ? Fraîchement sorti de l’École des Ponts et Chaussées dans les années 1830, l’ingénieur Auguste Mille, est affecté à la construction du grand phare de Sein. Dans la correspondance qu’il entretient avec sa hiérarchie parisienne, voici la description qu’il fait de son séjour insulaire : « Depuis deux ans, écrit-il, j’habite sur le triste et stérile rocher de l’Île de Sein ; depuis deux ans, je n’ai connu qu’une vie de dangers et de privations, logé dans une mauvaise bara...

  7. Digitaalse infosisu lepingutingimustele vastavuse hindamine. Euroopa ühise müügiõiguse eelnõu / Karin Sein, Siiri Kuusik

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2014-01-01

    Digitaalse infosisu kvaliteedi hindamisest, ligipääsust, tehnilistest kasutuspiirangutest, koostalitlusvõimest ja ühilduvusest. Autoriõigusest tingitud kasutuspiirangutest. Puudulikust informatsioonist ja turvalisusest. Artikkel põhineb osaliselt Siiri Kuusiku 2014. aasta kevadel kaitstud magistritööl

  8. Status of Kenyan Coral Reef lagoons. Project Report to Kenya Wildlife Services, Kenya Karine and Fisheries Researoh Institute & Kenya"s Fisheries Department.

    OpenAIRE

    McClanahan, T.; Muthiga, N.; Obura, D.; Mutere, J.; Mwachireya, S.

    1992-01-01

    This report presents the findings of Wildlife Conservation International's Coral Reef Conservation Project monitoring program of Kenyan coral reefs and suggest research and management plans for the coral reefs based on the findings.

  9. Kuidas hageda teises Euroopa Liidu liikmesriigis teadmata aadressil elavat võlgnikku? Kohtudokumentide kättetoimetamise ja tagaseljaotsusega seonduvad probleemid / Haide Hermet, Karin Sein

    Index Scriptorium Estoniae

    Hermet, Haide, 1970-

    2014-01-01

    Rahvusvahelisest kohtualluvusest Eesti tsiviilkohtumenetluse seadustiku § 75 lg 1 ja Brüsseli I määruse järgi. Kohtudokumentide kättetoimetamise määrusest 1393/2007. Euroopa Kohtu lahendites kehtestatud standarditest ja Eesti kohtupraktikast

  10. Kahjustatud isiku omaosalus kahju tekkimisel : [bakalaureusetöö] / Piia Karu ; Tartu Ülikooli Õigusinstituut ; juhendaja: Karin Sein

    Index Scriptorium Estoniae

    Karu, Piia

    2005-01-01

    Kahjuhüvitise vähendamine sõltuvalt kahjustatud isikust tulenevatest asjaoludest või ohust, mille eest kahjustatud isik vastutab ; kui kahjustatud isik aitas ise kahju tekkimisele kaasa või suurendas juba tekkinud kahju ; isiku surma põhjustamise, tema tervise kahjustamise või talle kehavigastuse tekitamise korral ; omaosaluse tagajärjed

  11. Eduard Tubina 100. sünniaastapäeva puhul toimus Stockholmis suur mälestuskontsert / Hilma Nerep-Mossin ; fotod: Karin Mickelsson

    Index Scriptorium Estoniae

    Nerep-Mossin, Hilma

    2005-01-01

    17. aprillil 2005 Stockholmi Soome Kirikus toimunud kontserdi muusikaarvustus. Esinesid Stockholmi Eesti Segakoor, Projektmeeskoor E. Tubin 100, laulusolistid Katrin Meerits, Liine Carlsson ja Mikko Pulkkinen

  12. Tarbijate õiguste kaitse Euroopa müügiõiguse eelnõus: kas kõrgem tase Eesti tarbija jaoks? / Karin Sein

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2013-01-01

    Euroopa Komisjoni ettepanekust luua vabatahtlik ühine Euroopa müügiõigus (EÜM) ning Eestis kehtivast regulatsioonist tulenevalt võlaõigusseadusest. Artikkel põhineb 32. Eesti õigusteadlaste päevadel peetud ettekandel, mille autor pidas Tartus 4. oktoobril 2012

  13. Välisriigis toimunud abielulahutuse tunnustamine Eestis : [magistritöö] / Rasmus Kuklane ; Tartu Ülikool, õigusteaduskond ; juhendaja: Karin Sein

    Index Scriptorium Estoniae

    Kuklane, Rasmus

    2008-01-01

    Välisriigis toimunud lahutuse tunnustamine Eestis Euroopa Liidu liikmesriigina (Brüssel II bis määrus) või rahvusvaheliste lepingute alusel (Haagi 1970. a. konventsioon, õigusabilepingud riikide vahel), kohtupraktika analüüs

  14. Müncheni eestlased tähistasid Eesti Vabariigi 93. sünnipäeva koos president Toomas Hendrik Ilvesega / Karin Ladva-Zoller

    Index Scriptorium Estoniae

    Ladva-Zoller, Karin, 1967-

    2011-01-01

    Münchenis Baieri resoranis Donisl toimus 6. veebr. 2011 Eesti Vabariigi 93. aastapäevale pühendatud pidu. Peol osalesid ka president Toomas Hendrik Ilves ja suursaadik Mart Laanemäe. President esines kõnega ja vastas Müncheni Eesti Kooli laste küsimustele

  15. Noored režissöörid väärtustavad eneseleidmist / Maiju Ingman, Anu Aun ; interv. Karin Klaus

    Index Scriptorium Estoniae

    Ingman, Maiju

    2007-01-01

    Pärnus näidatakse noortefilme : soomlanna Maiju Ingmani TÜ lõputööd "Mida iganes, Aleksander" (teekonnafilm, osades Jaak Prints, Arvo Kukumägi) ja Anu Auna lühifilmi "Indigo tuba" (mängivad Mirtel Pohla, Alo Kõrve). Režissöörid oma otsingutest ja filmidest

  16. Avalik ruum - kas hambutult kaitstud ühishuvi? / Martin Allik, Külli Volmer, Riin Alatalu ... [jt.] ; intervjueerinud Karin Paulus, Mari Kodres

    Index Scriptorium Estoniae

    2010-01-01

    Autorid selgitavad, mida tähendab nende jaoks avalik ruum, millised vastuolud võivad tekkida avalikus ruumis liikudes ja tegutsedes. Kärt Mere vastukaja on ilmunud: Eesti Kodanik, 2011, nr. 1, lk. 5, pealk.: Eakad linnaruumis

  17. Ebamäärasus kui eesmärk : "Coast Wise Europe'i" näitus ja konverents Rotermanni soolalaos / Karin Hallas

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    1998-01-01

    Aprillis eksponeeritud näitusest "Coast Wise Europe" ja 17. aprillil toimunud konverentsist "Ebamäärasus". David Milleri, Mart Raukase, Kaia Lehari, Inga Raukase, Kaarel Orviku ettekannetest. Ürituse korraldaja ja Eesti-poolse "Coast'i" eestvedaja Inga Raukas.

  18. Üürilepingu erakorraline ülesütlemine üürniku makseviivituse tõttu / Kaidi Urgas, Karin Sein

    Index Scriptorium Estoniae

    Urgas, Kaidi, 1989-

    2013-01-01

    Võlaõigusseaduse § 316 kohaldamise teoreetilistest alustest ja rakendamise praktikast Eesti kohtutes ning seaduse ajakohastamise vajadusest, et poolte õigused ja kohustused paremini tasakaalu viia. Üürileandja ülesütlemisõigus Šveitsi ja Saksa õiguses

  19. AFRICOM’s Role in Water Security

    Science.gov (United States)

    2012-06-01

    Niger River Basin,” USAID, December 2011, 16. 12. Roselle Henn et al., “The impact of Water as a United States Government Security Consideration for...Tensions,” A report to the German Advisory Council on Global Change, July 10, 2006, 3. 17. Ibid., 6. 18. Roselle Henn, et al., “The impact of Water as

  20. Disruption of Microtubules Post-Virus Entry Enhances Adeno-Associated Virus Vector Transduction

    Science.gov (United States)

    Xiao, Ping-Jie; Mitchell, Angela M.; Huang, Lu; Li, Chengwen; Samulski, R. Jude

    2016-01-01

    Perinuclear retention of viral particles is a poorly understood phenomenon observed during many virus infections. In this study, we investigated whether perinuclear accumulation acts as a barrier to limit recombinant adeno-associated virus (rAAV) transduction. After nocodazole treatment to disrupt microtubules at microtubule-organization center (MT-MTOC) after virus entry, we observed higher rAAV transduction. To elucidate the role of MT-MTOC in rAAV infection and study its underlying mechanisms, we demonstrated that rAAV's perinuclear localization was retained by MT-MTOC with fluorescent analysis, and enhanced rAAV transduction from MT-MTOC disruption was dependent on the rAAV capsid's nuclear import signals. Interestingly, after knocking down RhoA or inhibiting its downstream effectors (ROCK and Actin), MT-MTOC disruption failed to increase rAAV transduction or nuclear entry. These data suggest that enhancement of rAAV transduction is the result of increased trafficking to the nucleus via the RhoA-ROCK-Actin pathway. Ten-fold higher rAAV transduction was also observed by disrupting MT-MTOC in brain, liver, and tumor in vivo. In summary, this study indicates that virus perinuclear accumulation at MT-MTOC is a barrier-limiting parameter for effective rAAV transduction and defines a novel defense mechanism by which host cells restrain viral invasion. PMID:26942476

  1. Unrestricted Hepatocyte Transduction with Adeno-Associated Virus Serotype 8 Vectors in Mice

    Science.gov (United States)

    Nakai, Hiroyuki; Fuess, Sally; Storm, Theresa A.; Muramatsu, Shin-ichi; Nara, Yuko; Kay, Mark A.

    2005-01-01

    Recombinant adeno-associated virus (rAAV) vectors can mediate long-term stable transduction in various target tissues. However, with rAAV serotype 2 (rAAV2) vectors, liver transduction is confined to only a small portion of hepatocytes even after administration of extremely high vector doses. In order to investigate whether rAAV vectors of other serotypes exhibit similar restricted liver transduction, we performed a dose-response study by injecting mice with β-galactosidase-expressing rAAV1 and rAAV8 vectors via the portal vein. The rAAV1 vector showed a blunted dose-response similar to that of rAAV2 at high doses, while the rAAV8 vector dose-response remained unchanged at any dose and ultimately could transduce all the hepatocytes at a dose of 7.2 × 1012 vector genomes/mouse without toxicity. This indicates that all hepatocytes have the ability to process incoming single-stranded vector genomes into duplex DNA. A single tail vein injection of the rAAV8 vector was as efficient as portal vein injection at any dose. In addition, intravascular administration of the rAAV8 vector at a high dose transduced all the skeletal muscles throughout the body, including the diaphragm, the entire cardiac muscle, and substantial numbers of cells in the pancreas, smooth muscles, and brain. Thus, rAAV8 is a robust vector for gene transfer to the liver and provides a promising research tool for delivering genes to various target organs. In addition, the rAAV8 vector may offer a potential therapeutic agent for various diseases affecting nonhepatic tissues, but great caution is required for vector spillover and tight control of tissue-specific gene expression. PMID:15596817

  2. Novel strategy for generation and titration of recombinant adeno-associated virus vectors.

    Science.gov (United States)

    Shiau, Ai-Li; Liu, Pu-Ste; Wu, Chao-Liang

    2005-01-01

    Recombinant adeno-associated virus (rAAV) vectors have many advantages for gene therapeutic applications compared with other vector systems. Several methods that use plasmids or helper viruses have been reported for the generation of rAAV vectors. Unfortunately, the preparation of large-scale rAAV stocks is labor-intensive. Moreover, the biological titration of rAAV is still difficult, which may limit its preclinical and clinical applications. For this study, we developed a novel strategy to generate and biologically titrate rAAV vectors. A recombinant pseudorabies virus (PrV) with defects in its gD, gE, and thymidine kinase genes was engineered to express the AAV rep and cap genes, yielding PS virus, which served as a packaging and helper virus for the generation of rAAV vectors. PS virus was useful not only for generating high-titer rAAV vectors by cotransfection with an rAAV vector plasmid, but also for amplifying rAAV stocks. Notably, the biological titration of rAAV vectors was also feasible when cells were coinfected with rAAV and PS virus. Based on this strategy, we produced an rAAV that expresses prothymosin alpha (ProT). Expression of the ProT protein in vitro and in vivo mediated by rAAV/ProT gene transfer was detected by immunohistochemistry and a bioassay. Taken together, our results demonstrate that the PrV vector-based system is useful for generating rAAV vectors carrying various transgenes.

  3. Stable producer cell lines for adeno-associated virus (AAV) assembly.

    Science.gov (United States)

    Chadeuf, Gilliane; Salvetti, Anna

    2010-10-01

    Stable producer cell lines containing both the rep and cap genes and recombinant adeno-associated virus (rAAV) vectors can be infected with a helper virus to provide reliable and efficient production of rAAV stocks. However, the development of these cell lines is time-consuming. The procedure described here is therefore recommended only for studies requiring the production of high amounts of rAAV, such as preclinical studies performed in large animals.

  4. Die Rechtsstellung von Ausländern nach staatlichem Recht und Völkerrecht. Herausgegeben von Jochen Abr. Frowein und Torsten Stein. (Beiträge zum ausländischen öffentlichen Recht und Völkerrecht, Band 94) / Henn-Jüri Uibopuu

    Index Scriptorium Estoniae

    Uibopuu, Henn-Jüri, 1929-2012

    1988-01-01

    Raamatuarvustus: Die Rechtsstellung von Ausländern nach staatlichem Recht und Völkerrecht = The legal position of aliens in national and international law = Le régime juridique des étrangers en droit national et international / Max-Planck-Institut für ausländisches öffentliches Recht und Völkerrecht ; herausgegeben von Jochen A. Frowein, Torsten Stein. Berlin [etc.] : Springer, 1987

  5. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression in neurons

    Science.gov (United States)

    Saunders, Arpiar; Johnson, Caroline A.; Sabatini, Bernardo L.

    2012-01-01

    Understanding the organization of the nervous system requires methods for dissecting the contributions of each component cell type to circuit function. One widely used approach combines genetic targeting of Cre recombinase to specific cell populations with infection of recombinant adeno-associated viruses (rAAVs) whose transgene expression is activated by Cre (“Cre-On”). Distinguishing how the Cre-expressing neurons differ functionally from neighboring Cre-negative neurons requires rAAVs that are inactivated by Cre (“Cre-Off”) and can be used in tandem with Cre-On viruses. Here we introduce two rAAV vectors that are inactivated by Cre and carry different fluorophore and optogenetic constructs. We demonstrate single and dual rAAV systems to achieve Cre-On and Cre-Off expression in spatially-intermingled cell populations of the striatum. Using these systems, we uncovered cryptic genomic interactions that occur between multiple Cre-sensitive rAAVs or between Cre-sensitive rAAVs and somatic Cre-conditional alleles and devised methods to avoid these interactions. Our data highlight both important experimental caveats associated with Cre-dependent rAAV use as well as opportunities for the development of improved rAAVs for gene delivery. PMID:22866029

  6. Methods of treating Parkinson's disease using viral vectors

    Energy Technology Data Exchange (ETDEWEB)

    Bankiewicz, Krystof; Cunningham, Janet

    2016-11-15

    Methods of delivering viral vectors, particularly recombinant adeno-associated virus (rAAV) virions, to the central nervous system (CNS) using convection enhanced delivery (CED) are provided. The rAAV virions include a nucleic acid sequence encoding a therapeutic polypeptide. The methods can be used for treating CNS disorders such as for treating Parkinson's Disease.

  7. Effect and Mechanism of Mitomycin C Combined with Recombinant Adeno-Associated Virus Type II against Glioma

    Directory of Open Access Journals (Sweden)

    Hong Ma

    2013-12-01

    Full Text Available The effect of chemotherapy drug Mitomycin C (MMC in combination with recombinant adeno-associated virus II (rAAV2 in cancer therapy was investigated, and the mechanism of MMC affecting rAAV2’s bioactivity was also studied. The combination effect was evaluated by the level of GFP and TNF expression in a human glioma cell line, and the mechanism of MMC effects on rAAV mediated gene expression was investigated by AAV transduction related signal molecules. C57 and BALB/c nude mice were injected with rAAV-EGFP or rAAV-TNF alone, or mixed with MMC, to evaluate the effect of MMC on AAV-mediated gene expression and tumor suppression. MMC was shown to improve the infection activity of rAAV2 both in vitro and in vivo. Enhancement was found to be independent of initial rAAV2 receptor binding stage or subsequent second-strand synthesis of target DNA, but was related to cell cycle retardation followed by blocked genome degradation. In vivo injection of MMC combined with rAAV2 into the tumors of the animals resulted in significant suppression of tumor growth. It was thus demonstrated for the first time that MMC could enhance the expression level of the target gene mediated by rAAV2. The combination of rAAV2 and MMC may be a promising strategy in cancer therapy.

  8. Recombinant adeno-associated virus type 2 replication and packaging is entirely supported by a herpes simplex virus type 1 amplicon expressing Rep and Cap.

    Science.gov (United States)

    Conway, J E; Zolotukhin, S; Muzyczka, N; Hayward, G S; Byrne, B J

    1997-11-01

    Recombinant adeno-associated virus (AAV) type 2 (rAAV) vectors have recently been shown to have great utility as gene transfer agents both in vitro and in vivo. One of the problems associated with the use of rAAV vectors has been the difficulty of large-scale vector production. Low-efficiency plasmid transfection of the rAAV vector and complementing AAV type 2 (AAV-2) functions (rep and cap) followed by superinfection with adenovirus has been the standard approach to rAAV production. The objectives of this study were to demonstrate the ability of a recombinant herpes simplex virus type 1 (HSV-1) amplicon expressing AAV-2 Rep and Cap to support replication and packaging of rAAV vectors. HSV-1 amplicon vectors were constructed which contain the AAV-2 rep and cap genes under control of their native promoters (p5, p19, and p40). An HSV-1 amplicon vector, HSV-RC/KOS or HSV-RC/d27, was generated by supplying helper functions with either wild-type HSV-1 (KOS strain) or the ICP27-deleted mutant of HSV-1, d27-1, respectively. Replication of the amplicon stocks is not inhibited by the presence of AAV-2 Rep proteins, which highlights important differences between HSV-1 and adenovirus replication and the mechanism of providing helper function for productive AAV infection. Coinfection of rAAV and HSV-RC/KOS resulted in the replication and amplification of rAAV genomes. Similarly, rescue and replication of rAAV genomes occurred when rAAV vector plasmids were transfected into cells followed by HSV-RC/KOS infection and when two rAAV proviral cell lines were infected with HSV-RC/KOS or HSV-RC/d27. Production of infectious rAAV by rescue from two rAAV proviral cell lines has also been achieved with HSV-RC/KOS and HSV-RC/d27. The particle titer of rAAV produced with HSV-RC/d27 is equal to that achieved by supplying rep and cap by transfection followed by adenovirus superinfection. Importantly, no detectable wild-type AAV-2 is generated with this approach. These results demonstrate

  9. Factors influencing recombinant adeno-associated virus production.

    Science.gov (United States)

    Salvetti, A; Orève, S; Chadeuf, G; Favre, D; Cherel, Y; Champion-Arnaud, P; David-Ameline, J; Moullier, P

    1998-03-20

    Recombinant adeno-associated virus (rAAV) is produced by transfecting cells with two constructs: the rAAV vector plasmid and the rep-cap plasmid. After subsequent adenoviral infection, needed for rAAV replication and assembly, the virus is purified from total cell lysates through CsCl gradients. Because this is a long and complex procedure, the precise titration of rAAV stocks, as well as the measure of the level of contamination with adenovirus and rep-positive AAV, are essential to evaluate the transduction efficiency of these vectors in vitro and in vivo. Our vector core is in charge of producing rAAV for outside investigators as part of a national network promoted by the Association Française contre les Myopathies/Généthon. We report here the characterization of 18 large-scale rAAV stocks produced during the past year. Three major improvements were introduced and combined in the rAAV production procedure: (i) the titration and characterization of rAAV stocks using a stable rep-cap HeLa cell line in a modified Replication Center Assay (RCA); (ii) the use of different rep-cap constructs to provide AAV regulatory and structural proteins; (iii) the use of an adenoviral plasmid to provide helper functions needed for rAAV replication and assembly. Our results indicate that: (i) rAAV yields ranged between 10(11) to 5 x 10(12) total particles; (ii) the physical particle to infectious particle (measured by RCA) ratios were consistently below 50 when using a rep-cap plasmid harboring an ITR-deleted AAV genome; the physical particle to transducing particle ratios ranged between 400 and 600; (iii) the use of an adenoviral plasmid instead of an infectious virion did not affect the particles or the infectious particles yields nor the above ratio. Most of large-scale rAAV stocks (7/9) produced using this plasmid were free of detectable infectious adenovirus as determined by RCA; (iv) all the rAAV stocks were contaminated with rep-positive AAV as detected by RCA. In summary

  10. Parts ei soovi Res Publica esimehe ametit / Tuuli Koch

    Index Scriptorium Estoniae

    Koch, Tuuli

    2005-01-01

    Kõige tõenäolisem kandidaat Res Publica juhi kohale on Taavi Veskimägi, aseesimeesteks kandideerivad Marko Mihkelson ja Henn Pärn. Res Publica fraktsiooni koosolekul leidis Mihhail Lotman, et erakond võiks olla feminiinsem

  11. New Estonian CDs / Igor Garshnek

    Index Scriptorium Estoniae

    Garšnek, Igor, 1958-

    2001-01-01

    Uutest heliplaatidest "Eesti portreed", Lepo Sumera. Film Music by Lepo Sumera, Urmas Sisask "Starry Sky Cycle", "Poeme d'amour", "Tango King Astor Piazolla", "Eduard Tubin. Complete Symphonies vol. 2", Henn Rebane "Arabesque", Eino Tamberg "Nocturne"

  12. Kirjastusuudiseid

    Index Scriptorium Estoniae

    2007-01-01

    Uutest kunstiraamatutest "Meistriteoste lummus. Koopia 19. sajandil" (koostaja Tiina-Mall Kreem), "Priit Pärn" (koostaja Eha Komissarov), "Henn Roode. Modernist saatuse kiuste" (koostaja Kädi Talvoja)

  13. Notes on bolete taxonomy—II

    NARCIS (Netherlands)

    Singer, Rolf

    1978-01-01

    Newly discovered mycorrhizal relationships of boletes with Sapotaceae and Nyctaginaceae in the Neotropics are discussed. The eight neotropical species of Phylloporus are keyed out and three described. Fistulinella Henn. is transferred to the Strobilomycetaceae. Phylloporus manausensis Sing. and P.

  14. Siidii / Kreete Reval

    Index Scriptorium Estoniae

    Reval, Kreete

    2003-01-01

    Heliplaatidest: Velvet Legion "Velvet Legion", Lou Reed "The Raven", Forgotten Sunrise "Please Disconnect Me", Ry Cooder & Manuel Galban "Mambo Sinuendo", Robert Jürjendal & Henn Rebane "Eesti Asi", Melanie C "Reason"

  15. Modernse õpetlase kutsumusest ja selle puudumisest / Hent Kalmo

    Index Scriptorium Estoniae

    Kalmo, Hent, 1979-

    2011-01-01

    Arvustus: Weber, Max. Poliitika kui elukutse ja kutsumus ; Teadus kui elukutse ja kutsumus / tõlkijad: Jaan Isotamm, Henn Käärik, Kaia Sisask. Tallinn : TLU Press, 2010. (Bibliotheca controversiarum)

  16. [Max Weber. Poliitika kui elukutse ja kutsumus. Teadus kui elukutse ja kutsumus] / Hermo Kuusk

    Index Scriptorium Estoniae

    Kuusk, Hermo, 1976-

    2011-01-01

    Tutvustus: Weber, Max. Poliitika kui elukutse ja kutsumus ; Teadus kui elukutse ja kutsumus / tõlkijad: Jaan Isotamm, Henn Käärik, Kaia Sisask. Tallinn : TLU Press, 2010. (Bibliotheca controversiarum)

  17. Teadlase elukutse kui missioon ja pühendumine / Rein Ruutsoo

    Index Scriptorium Estoniae

    Ruutsoo, Rein, 1947-

    2011-01-01

    Tutvustus: Weber, Max. Poliitika kui elukutse ja kutsumus ; Teadus kui elukutse ja kutsumus / tõlkijad: Jaan Isotamm, Henn Käärik, Kaia Sisask. Tallinn : TLU Press, 2010. (Bibliotheca controversiarum)

  18. August toob Vainupeale kontserte

    Index Scriptorium Estoniae

    2006-01-01

    Kontsertidest Vainupeal: Kunda suvekooli noored saksofonistid 10. aug., Jaan Arder ja Henn Rebane 15. aug., kunstinäituse "Blended X" sulgemiskontsert 19. aug., Silvi Vrait ja Jaak Jürisson 26. augustil

  19. Firmade ost-müük annab tööd / Ketlin Priilinn

    Index Scriptorium Estoniae

    Priilinn, Ketlin, 1982-

    2004-01-01

    TOP-i 2. koht. Rimess MRI on Eesti erakapitalil põhinev audiitorkontrolli ja finantsnõustamise teenuseid osutav ettevõte, kelle kliendinimistus on üle 800 kliendi. Diagramm. Kommenteerib Henn Ruubel

  20. Tihedaks dopingukontrolliks napib raha / Inga Höglund

    Index Scriptorium Estoniae

    Höglund, Inga

    2008-01-01

    Eesti võrkpallureid pole kodus juba aastaid testitud. Kommenteerivad Eesti Võrkpalli Liidu peasekretär Henn Vallimäe, spordiarst Mihkel Mardna ja SA Eesti Antidopingu juht Kristel Berendsen. Lisa: Antidoping

  1. Cre Activated and Inactivated Recombinant Adeno-Associated Viral Vectors for Neuronal Anatomical Tracing or Activity Manipulation.

    Science.gov (United States)

    Saunders, Arpiar; Sabatini, Bernardo L

    2015-07-01

    Recombinant adeno-associated viruses (rAAVs) transcriptionally activated by Cre recombinase (Cre-On) are powerful tools for determining the anatomy and function of genetically defined neuronal types in transgenic Cre driver mice. Here we describe how rAAVs transcriptionally inactivated by Cre (Cre-Off) can be used in conjunction with Cre-On rAAVs or genomic Cre-reporter alleles to study brain circuits. Intracranial injection of Cre-On/Cre-Off rAAVs into spatially intermingled Cre(+) and Cre(-) neurons allows these populations to be differentially labeled or manipulated within individual animals. This comparison helps define the unique properties of Cre(+) neurons, highlighting the specialized role they play in their constituent brain circuits. This protocol touches on the conceptual and experimental background of Cre-Off rAAV systems, including caveats and methods of validation. Copyright © 2015 John Wiley & Sons, Inc.

  2. The Role of Stat3 Activation in Androgen Receptor Signaling and Prostate Cancer

    Science.gov (United States)

    2006-07-01

    S. C. & Xiao, G. (2003) Cancer Metastasis Rev. 22, 405–422. 10. Karin, M. & Greten , F. R. (2005) Nat. Rev. Immunol. 5, 749–759. 11. Karin, M., Cao, Y... Greten , F. R. & Li, Z. W. (2002) Nat. Rev. Cancer 2, 301–310. 12. Fan, C. M. & Maniatis, T. (1991) Nature 354, 395–398. 13. Betts, J. C. & Nabel, G

  3. New Quasi Low-Dimensional 4d and 5d Transition Metal Oxides with Correlated Electronic Properties - Synthesis and Characterizations

    Science.gov (United States)

    2016-02-17

    Rong Li, Artem M. Abakumov, Mark. Croft, Alexander Ignatov, Peter W Stephens, Jason P. Hodges, P?emysl Van?k, Craig J. Fennie ,, Karin M. Rabe...Brian Craig Fennie , Karin Rabe, Stanislav Kamba and M. Greenblatt, submitted to Inorg. Chem. 1-2016 14. First evidence of the multiferroicity in

  4. The upstream enhancer elements of the G6PC promoter are critical for optimal G6PC expression in murine glycogen storage disease type Ia.

    Science.gov (United States)

    Lee, Young Mok; Pan, Chi-Jiunn; Koeberl, Dwight D; Mansfield, Brian C; Chou, Janice Y

    2013-11-01

    Glycogen storage disease type-Ia (GSD-Ia) patients deficient in glucose-6-phosphatase-α (G6Pase-α or G6PC) manifest impaired glucose homeostasis characterized by fasting hypoglycemia, growth retardation, hepatomegaly, nephromegaly, hyperlipidemia, hyperuricemia, and lactic acidemia. Two efficacious recombinant adeno-associated virus pseudotype 2/8 (rAAV8) vectors expressing human G6Pase-α have been independently developed. One is a single-stranded vector containing a 2864-bp of the G6PC promoter/enhancer (rAAV8-GPE) and the other is a double-stranded vector containing a shorter 382-bp minimal G6PC promoter/enhancer (rAAV8-miGPE). To identify the best construct, a direct comparison of the rAAV8-GPE and the rAAV8-miGPE vectors was initiated to determine the best vector to take forward into clinical trials. We show that the rAAV8-GPE vector directed significantly higher levels of hepatic G6Pase-α expression, achieved greater reduction in hepatic glycogen accumulation, and led to a better toleration of fasting in GSD-Ia mice than the rAAV8-miGPE vector. Our results indicated that additional control elements in the rAAV8-GPE vector outweigh the gains from the double-stranded rAAV8-miGPE transduction efficiency, and that the rAAV8-GPE vector is the current choice for clinical translation in human GSD-Ia. © 2013.

  5. Hermite Functional Link Neural Network for Solving the Van der Pol-Duffing Oscillator Equation.

    Science.gov (United States)

    Mall, Susmita; Chakraverty, S

    2016-08-01

    Hermite polynomial-based functional link artificial neural network (FLANN) is proposed here to solve the Van der Pol-Duffing oscillator equation. A single-layer hermite neural network (HeNN) model is used, where a hidden layer is replaced by expansion block of input pattern using Hermite orthogonal polynomials. A feedforward neural network model with the unsupervised error backpropagation principle is used for modifying the network parameters and minimizing the computed error function. The Van der Pol-Duffing and Duffing oscillator equations may not be solved exactly. Here, approximate solutions of these types of equations have been obtained by applying the HeNN model for the first time. Three mathematical example problems and two real-life application problems of Van der Pol-Duffing oscillator equation, extracting the features of early mechanical failure signal and weak signal detection problems, are solved using the proposed HeNN method. HeNN approximate solutions have been compared with results obtained by the well known Runge-Kutta method. Computed results are depicted in term of graphs. After training the HeNN model, we may use it as a black box to get numerical results at any arbitrary point in the domain. Thus, the proposed HeNN method is efficient. The results reveal that this method is reliable and can be applied to other nonlinear problems too.

  6. Vahetusüliõpilased Helsingi ülikooli ja Jyväskylä ülikooli on Gerli Jõgi, Karin Leemet, Ott Lumi, Tarmo Tuule ja Siret Voll

    Index Scriptorium Estoniae

    2000-01-01

    Vastavalt Tallinna Pedagoogikaülikooli ning Helsingi ja Jyväskylä ülikooli vahel sõlmitud koostöölepingutele on igal aastal neljal-viiel üliõpilasel võimalus õppida semester partnerülikoolis

  7. Kes peab posti- või Interneti-müügi lepingust taganemisel kandma esialgsed postikulud? : Euroopa Kohtu otsus asjas Handelsgesellschaft Heinrich Heine GmbH vs. Verbraucherzentrale Nordrhein-Westfalen e.V. / Karin Sein

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2010-01-01

    Euroopa Kohtu lahendist, mille kohaselt on Euroopa Liidu kaugmüügidirektiiviga vastuolus müügilepingu klauslid, mille järgi lepingust taganemise korral esialgseid postikulusid tellijatele ei tagastata

  8. Tarbijalepingute kohtualluvuse probleemid Brüsseli I määruses: milliste ohtudega peavad arvestama oma kaupu või teenuseid piiriüleselt pakkuvad ettevõtjad? / Karin Sein

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2014-01-01

    Millal võib ühes liikmesriigis elav tarbija, kes on ostnud kauba või saanud teenuse teises liikmesriigis asuvalt ettevõtjalt, esitada ettevõtja vastu hagi oma elukohariigi kohtusse. Euroopa Kohtu lahenditest kohtuasjades Alpenhof, Mühlleitner ja Emrek

  9. Appi - tarbijast võlgnik kolis välismaale! : kommentaar Riigikohtu lahenditele asjades 3-2-1-114-12 ja 3-2-1-123-12 / Karin Sein

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2013-01-01

    Riigikohtu lahendist 3-2-1-114-12, milles vaieldi füüsilisest isikust võlgniku pankroti väljakuulutamise kohtualluvuse üle. Lahendist 3-2-1-123-12, milles vaieldi, kas ja millistel tingimustel on Eesti kohus pädev läbi vaatama hagi Eestist lahkunud tarbijast krediidisaaja vastu

  10. Õigus oma kohtuasja arutamisele seadusega määratud kohtus kui põhiõigus ja kohtuasjade juhusliku jaotamise põhimõte : [bakalaureusetöö] / Karin Antsov ; Tartu Ülikool, õigusteaduskond ; juhendaja: Eerik Kergandberg

    Index Scriptorium Estoniae

    Antsov, Karin, 1979-

    2003-01-01

    Õigus seadusega määratud kohtule Euroopa Inimõiguste konventsioonis (art. 6, lõige 1), Eesti Vabariigi põhiseaduses (§24, lõige 1), kohtualluvuse regulatsioon Eesti menetlusseadustikes, kohtuasjade statistiline jagamine, rahvakohtunike kaasamine

  11. Kohalike omavalitsuste investeeringud ja finantsvõimekus : olukord ja selle mõju arendustegevusele = Investments and financial capability of local governments : situation and its influence on development activities / Karin-Liis Haljaste, Rivo Noork

    Index Scriptorium Estoniae

    Haljaste, Karin-Liis

    2007-01-01

    Eesti kohaliku omavalitsuse süsteemis on avalike ülesannete täitmise kavandamisel keskne koht valla või linna arengukaval, mille sidumist valla- või linnaeelarvega võimaldavad tegevuskava, eelarveprognoos ja -strateegia. Strateegiline kavandamine, investeeringud, analüüs. Lisatud tabelid ja graafikud

  12. Leping kinnisvaraarendaja ja kinnisasja omandaja vahel : kas müügileping või segatüüpi leping? Kommentaar Riigikohtu otsusele tsiviilasjas 3-2-1-98-09 / Karin Sein

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2010-01-01

    Riigikohtu lahendist 3-2-1-98-09: A & M Invest OÜ kassatsioonkaebus Tallinna Ringkonnakohtu 30.03.2009. a otsusele A & M Invest OÜ hagis K. Radiku ja M. Radiku vastu hüvitise 1 950 946,70 krooni väljamõistmiseks

  13. Empty virions in AAV8 vector preparations reduce transduction efficiency and may cause total viral particle dose-limiting side effects

    Directory of Open Access Journals (Sweden)

    Kai Gao

    2014-01-01

    Full Text Available Empty virions are inadvertent by-products of recombinant adeno-associated virus (rAAV packaging process, resulting in vector lots with mixtures of full and empty virions at variable ratios. Impact of empty virions on the efficiency and side effects of rAAV transduction has not been well characterized. Here, we generated partially and completely empty AAV8 virions, fully packaged rAAV8 lots, and mixtures of empty and fully packaged virions with variable ratios of empty virions. The aforementioned dosing formulations of rAAV8 expressing either cellular (EGFP (enhanced green fluorescent protein or nuclear-targeted (n LacZ or secreted (human α1-antitrypsin (hA1AT reporter genes were intravenously injected into two different mouse strains, followed by analyses of transgene expressions and serum alanine aminotransferase (ALT levels at different time points. We found that addition of empty particles to the fixed doses of rAAV8 preparations repressed liver transduction up to 64% (serum hA1AT and 44% (nLacZ in C57BL/6 mice, respectively. The similar trend in inhibiting EGFP expression together with concurrent elevations of serum ALT levels were observed in the BALB/c mice, indicating that empty particles may also exacerbate side effects of rAAV8 EGFP transduction. Our results suggest that removal of empty particles from rAAV preparations may improve efficacy and safety of AAV in clinical applications.

  14. High-titer recombinant adeno-associated virus production utilizing a recombinant herpes simplex virus type I vector expressing AAV-2 Rep and Cap.

    Science.gov (United States)

    Conway, J E; Rhys, C M; Zolotukhin, I; Zolotukhin, S; Muzyczka, N; Hayward, G S; Byrne, B J

    1999-06-01

    Recombinant adeno-associated virus type 2 (rAAV) vectors have recently been used to achieve long-term, high level transduction in vivo. Further development of rAAV vectors for clinical use requires significant technological improvements in large-scale vector production. In order to facilitate the production of rAAV vectors, a recombinant herpes simplex virus type I vector (rHSV-1) which does not produce ICP27, has been engineered to express the AAV-2 rep and cap genes. The optimal dose of this vector, d27.1-rc, for AAV production has been determined and results in a yield of 380 expression units (EU) of AAV-GFP produced from 293 cells following transfection with AAV-GFP plasmid DNA. In addition, d27.1-rc was also efficient at producing rAAV from cell lines that have an integrated AAV-GFP provirus. Up to 480 EU/cell of AAV-GFP could be produced from the cell line GFP-92, a proviral, 293 derived cell line. Effective amplification of rAAV vectors introduced into 293 cells by infection was also demonstrated. Passage of rAAV with d27. 1-rc results in up to 200-fold amplification of AAV-GFP with each passage after coinfection of the vectors. Efficient, large-scale production (>109 cells) of AAV-GFP from a proviral cell line was also achieved and these stocks were free of replication-competent AAV. The described rHSV-1 vector provides a novel, simple and flexible way to introduce the AAV-2 rep and cap genes and helper virus functions required to produce high-titer rAAV preparations from any rAAV proviral construct. The efficiency and potential for scalable delivery of d27.1-rc to producer cell cultures should facilitate the production of sufficient quantities of rAAV vectors for clinical application.

  15. A novel and highly efficient production system for recombinant adeno-associated virus vector.

    Science.gov (United States)

    Wu, Zhijian; Wu, Xiaobing; Cao, Hui; Dong, Xiaoyan; Wang, Hong; Hou, Yunde

    2002-02-01

    Recombinant adeno-associated virus (rAAV) has proven to be a promising gene delivery vector for human gene therapy. However, its application has been limited by difficulty in obtaining enough quantities of high-titer vector stocks. In this paper, a novel and highly efficient production system for rAAV is described. A recombinant herpes simplex virus type 1 (rHSV-1) designated HSV1-rc/DeltaUL2, which expressed adeno-associated virus type2 (AAV-2) Rep and Cap proteins, was constructed previously. The data confirmed that its functions were to support rAAV replication and packaging, and the generated rAAV was infectious. Meanwhile, an rAAV proviral cell line designated BHK/SG2, which carried the green fluorescent protein (GFP) gene expression cassette, was established by transfecting BHK-21 cells with rAAV vector plasmid pSNAV-2-GFP. Infecting BHK/SG2 with HSV1-rc/DeltaUL2 at an MOI of 0.1 resulted in the optimal yields of rAAV, reaching 250 transducing unit (TU) or 4.28x10(4) particles per cell. Therefore, compared with the conventional transfection method, the yield of rAAV using this "one proviral cell line, one helper virus" strategy was increased by two orders of magnitude. Large-scale production of rAAV can be easily achieved using this strategy and might meet the demands for clinical trials of rAAV-mediated gene therapy.

  16. Process optimization of large-scale production of recombinant adeno-associated vectors using dielectric spectroscopy.

    Science.gov (United States)

    Negrete, Alejandro; Esteban, Geoffrey; Kotin, Robert M

    2007-09-01

    A well-characterized manufacturing process for the large-scale production of recombinant adeno-associated vectors (rAAV) for gene therapy applications is required to meet current and future demands for pre-clinical and clinical studies and potential commercialization. Economic considerations argue in favor of suspension culture-based production. Currently, the only feasible method for large-scale rAAV production utilizes baculovirus expression vectors and insect cells in suspension cultures. To maximize yields and achieve reproducibility between batches, online monitoring of various metabolic and physical parameters is useful for characterizing early stages of baculovirus-infected insect cells. In this study, rAAVs were produced at 40-l scale yielding ~1 x 10(15) particles. During the process, dielectric spectroscopy was performed by real time scanning in radio frequencies between 300 kHz and 10 MHz. The corresponding permittivity values were correlated with the rAAV production. Both infected and uninfected reached a maximum value; however, only infected cell cultures permittivity profile reached a second maximum value. This effect was correlated with the optimal harvest time for rAAV production. Analysis of rAAV indicated the harvesting time around 48 h post-infection (hpi), and 72 hpi produced similar quantities of biologically active rAAV. Thus, if operated continuously, the 24-h reduction in the production process of rAAV gives sufficient time for additional 18 runs a year corresponding to an extra production of ~2 x 10(16) particles. As part of large-scale optimization studies, this new finding will facilitate the bioprocessing scale-up of rAAV and other bioproducts.

  17. Toward exascale production of recombinant adeno-associated virus for gene transfer applications.

    Science.gov (United States)

    Cecchini, S; Negrete, A; Kotin, R M

    2008-06-01

    To gain acceptance as a medical treatment, adeno-associated virus (AAV) vectors require a scalable and economical production method. Recent developments indicate that recombinant AAV (rAAV) production in insect cells is compatible with current good manufacturing practice production on an industrial scale. This platform can fully support development of rAAV therapeutics from tissue culture to small animal models, to large animal models, to toxicology studies, to Phase I clinical trials and beyond. Efforts to characterize, optimize and develop insect cell-based rAAV production have culminated in successful bioreactor-scale production of rAAV, with total yields potentially capable of approaching the exa-(10(18)) scale. These advances in large-scale AAV production will allow us to address specific catastrophic, intractable human diseases such as Duchenne muscular dystrophy, for which large amounts of recombinant vector are essential for successful outcome.

  18. Mida sportijad vajavad? / Evelin Kivilo-Paas

    Index Scriptorium Estoniae

    Kivilo-Paas, Evelin, 1979-

    2015-01-01

    Sportijate toitainete vajaduse kohta jagavad selgitusi Tallinna Ülikooli terviseteaduste ja spordi instituudi direktor ja spordibioloog Kristjan Port, Synlab Eesti laboriarst dr Anneli Raave-Sepp ja Spordimeditsiini SA spordiarst dr Krista Veevo

  19. Convection Enhanced Delivery of Recombinant Adeno-associated Virus into the Mouse Brain.

    Science.gov (United States)

    Nash, Kevin R; Gordon, Marcia N

    2016-01-01

    Recombinant adeno-associated virus (rAAV) has become an extremely useful tool for the study of gene over expression or knockdown in the central nervous system of experimental animals. One disadvantage of intracranial injections of rAAV vectors into the brain parenchyma has been restricted distribution to relatively small volumes of the brain. Convection enhanced delivery (CED) is a method for delivery of clinically relevant amounts of therapeutic agents to large areas of the brain in a direct intracranial injection procedure. CED uses bulk flow to increase the hydrostatic pressure and thus improve volume distribution. The CED method has shown robust gene transfer and increased distribution within the CNS and can be successfully used for different serotypes of rAAV for increased transduction of the mouse CNS. This chapter details the surgical injection of rAAV by CED into a mouse brain.

  20. Adeno-Associated Virus-Mediated Correction of a Canine Model of Glycogen Storage Disease Type Ia

    Science.gov (United States)

    Weinstein, David A.; Correia, Catherine E.; Conlon, Thomas; Specht, Andrew; Verstegen, John; Onclin-Verstegen, Karine; Campbell-Thompson, Martha; Dhaliwal, Gurmeet; Mirian, Layla; Cossette, Holly; Falk, Darin J.; Germain, Sean; Clement, Nathalie; Porvasnik, Stacy; Fiske, Laurie; Struck, Maggie; Ramirez, Harvey E.; Jordan, Juan; Andrutis, Karl; Chou, Janice Y.; Byrne, Barry J.

    2010-01-01

    Abstract Glycogen storage disease type Ia (GSDIa; von Gierke disease; MIM 232200) is caused by a deficiency in glucose-6-phosphatase-α. Patients with GSDIa are unable to maintain glucose homeostasis and suffer from severe hypoglycemia, hepatomegaly, hyperlipidemia, hyperuricemia, and lactic acidosis. The canine model of GSDIa is naturally occurring and recapitulates almost all aspects of the human form of disease. We investigated the potential of recombinant adeno-associated virus (rAAV) vector-based therapy to treat the canine model of GSDIa. After delivery of a therapeutic rAAV2/8 vector to a 1-day-old GSDIa dog, improvement was noted as early as 2 weeks posttreatment. Correction was transient, however, and by 2 months posttreatment the rAAV2/8-treated dog could no longer sustain normal blood glucose levels after 1 hr of fasting. The same animal was then dosed with a therapeutic rAAV2/1 vector delivered via the portal vein. Two months after rAAV2/1 dosing, both blood glucose and lactate levels were normal at 4 hr postfasting. With more prolonged fasting, the dog still maintained near-normal glucose concentrations, but lactate levels were elevated by 9 hr, indicating that partial correction was achieved. Dietary glucose supplementation was discontinued starting 1 month after rAAV2/1 delivery and the dog continues to thrive with minimal laboratory abnormalities at 23 months of age (18 months after rAAV2/1 treatment). These results demonstrate that delivery of rAAV vectors can mediate significant correction of the GSDIa phenotype and that gene transfer may be a promising alternative therapy for this disease and other genetic diseases of the liver. PMID:20163245

  1. Construction of adeno-associated virus packaging plasmids and cells that directly select for AAV helper functions.

    Science.gov (United States)

    Whiteway, Alistair; Deru, Wale; Prentice, H Grant; Anderson, Robert

    2003-12-01

    Recombinant adeno-associated virus type 2 (rAAV) has promise for use as a gene therapy vector. Potential problems in the production of rAAV stocks are both the limited amount of recombinant virus that is produced by traditional methods and the possibility of wild-type replication competent adeno-associated virus (wtAAV) contamination. The presence of these contaminants is largely dependent upon the helper plasmid used. Whilst wtAAV is not a pathogen, the presence of these contaminants is undesirable as they may affect experiments concerning the biology of rAAV. Additionally as protocols using rAAV with altered tropism are becoming more prevalent, it is important that no recombination be permitted that may cause the creation of a replication competent AAV with modified (targeting) capsids. Many experimental protocols require the generation of large amounts of high titre rAAV stocks. We describe the production of several AAV helper plasmids and cell lines designed to achieve this goal. These plasmids possess split AAV rep and cap genes to eliminate the production of wtAAV and they possess a selection mechanism which is operatively linked to expression from the AAV cap gene. This allows positive selection of those cells expressing the highest level of the structural capsid proteins and therefore those cells which yield the highest amount of rAAV.

  2. Viral single-strand DNA induces p53-dependent apoptosis in human embryonic stem cells.

    Science.gov (United States)

    Hirsch, Matthew L; Fagan, B Matthew; Dumitru, Raluca; Bower, Jacquelyn J; Yadav, Swati; Porteus, Matthew H; Pevny, Larysa H; Samulski, R Jude

    2011-01-01

    Human embryonic stem cells (hESCs) are primed for rapid apoptosis following mild forms of genotoxic stress. A natural form of such cellular stress occurs in response to recombinant adeno-associated virus (rAAV) single-strand DNA genomes, which exploit the host DNA damage response for replication and genome persistence. Herein, we discovered a unique DNA damage response induced by rAAV transduction specific to pluripotent hESCs. Within hours following rAAV transduction, host DNA damage signaling was elicited as measured by increased gamma-H2AX, ser15-p53 phosphorylation, and subsequent p53-dependent transcriptional activation. Nucleotide incorporation assays demonstrated that rAAV transduced cells accumulated in early S-phase followed by the induction of apoptosis. This lethal signaling sequalae required p53 in a manner independent of transcriptional induction of Puma, Bax and Bcl-2 and was not evident in cells differentiated towards a neural lineage. Consistent with a lethal DNA damage response induced upon rAAV transduction of hESCs, empty AAV protein capsids demonstrated no toxicity. In contrast, DNA microinjections demonstrated that the minimal AAV origin of replication and, in particular, a 40 nucleotide G-rich tetrad repeat sequence, was sufficient for hESC apoptosis. Our data support a model in which rAAV transduction of hESCs induces a p53-dependent lethal response that is elicited by a telomeric sequence within the AAV origin of replication.

  3. Viral single-strand DNA induces p53-dependent apoptosis in human embryonic stem cells.

    Directory of Open Access Journals (Sweden)

    Matthew L Hirsch

    Full Text Available Human embryonic stem cells (hESCs are primed for rapid apoptosis following mild forms of genotoxic stress. A natural form of such cellular stress occurs in response to recombinant adeno-associated virus (rAAV single-strand DNA genomes, which exploit the host DNA damage response for replication and genome persistence. Herein, we discovered a unique DNA damage response induced by rAAV transduction specific to pluripotent hESCs. Within hours following rAAV transduction, host DNA damage signaling was elicited as measured by increased gamma-H2AX, ser15-p53 phosphorylation, and subsequent p53-dependent transcriptional activation. Nucleotide incorporation assays demonstrated that rAAV transduced cells accumulated in early S-phase followed by the induction of apoptosis. This lethal signaling sequalae required p53 in a manner independent of transcriptional induction of Puma, Bax and Bcl-2 and was not evident in cells differentiated towards a neural lineage. Consistent with a lethal DNA damage response induced upon rAAV transduction of hESCs, empty AAV protein capsids demonstrated no toxicity. In contrast, DNA microinjections demonstrated that the minimal AAV origin of replication and, in particular, a 40 nucleotide G-rich tetrad repeat sequence, was sufficient for hESC apoptosis. Our data support a model in which rAAV transduction of hESCs induces a p53-dependent lethal response that is elicited by a telomeric sequence within the AAV origin of replication.

  4. Pronksiööl küpsenud valus tõde / Gerda Kordemets ; intervjueeris Jüri Aarma

    Index Scriptorium Estoniae

    Kordemets, Gerda, 1960-

    2009-01-01

    ETV lavastuslike saadete toimetuse juhataja meenutab Tallinna Linnateatri lavastuse "Karin ja Indrek. Tõde ja õigus" Eesti Televisioonile lindistamist aprillis 2007. Lavastaja Elmo Nüganen, aluseks Tammsaare "Tõe ja õiguse" IV osa

  5. Professor Peep Veski meie meenutustes

    Index Scriptorium Estoniae

    2014-01-01

    Mälestusi Tartu Ülikooli Farmaatsia instituudi juhatajast Peep Veskist jagavad: Eve-Irene Lepist, Urve Paaver, Karin Kogermann, Kristian Semjonov, Jaak Koppel, Kristin Raudsepp, Imre Klebovich, Filiz Hincal, A. Atilla Hincal ja Elmar Arak

  6. Disaini abil edukaks / Bo Englund ; interv. Kai Lobjakas

    Index Scriptorium Estoniae

    Englund, Bo

    2005-01-01

    Euroopa Disainiühenduste büroost (Bureau of European Design Association - BEDA), rahvuslikust disainipoliitikast, Rootsi-Eesti disainikonverentsist "Innovatsioon läbi disaini - disaini oma tulevik" Viinistu Kunstimuuseumis. Kommentaar Karin Pauluselt

  7. Eerosest valgustatud / Rein Veidemann

    Index Scriptorium Estoniae

    Veidemann, Rein, 1946-

    2002-01-01

    Arvustus: Saarsen, Karin. Lõvi ja orhidee : [luuletused]. Tallinn : Eesti Raamat, 2002 ; Under, Marie. Under armastusest : [luuletused ja katkendid kirjadest] / valik ja järelsõna: Sirje Olesk. Tallinn : Tänapäev, 2002

  8. Advent Euroopa viienda õiega

    Index Scriptorium Estoniae

    1999-01-01

    Esimesel advendil Tallinnas toimunud Taivo Pilleri jõuludemonstratsioonil osalesid edukalt ka kaks Ida-Viru lilleseadjat: Karin Lainjärv ja Marietta Rossmann; paremateks tunnistati Irmen Laving, Saale Halla, Heidi Haggi

  9. Muzei KUMU priglashajet russkih utshenikov / Andrei Babin

    Index Scriptorium Estoniae

    Babin, Andrei

    2007-01-01

    KUMU hariduskeskuse uus projekt "Remark"aitab vene õppekeelega koolide õpilastel ja lasteaedade lastel põhjalikumalt tutvuda eesti kunstiklassikaga. Kommenteerivad projekti koordinaator Jelena Levtshenkova ja programmijuht Karin Taidre

  10. Välieluruumi ajakiri Õu = Õu, the Landscape Architecture Magazine

    Index Scriptorium Estoniae

    2016-01-01

    Välieluruumi ajakirjast "Õu", peatoimetaja Anna-Liisa Unt, kolleegium Merle Karro-Kalberg ja Karin Bachmann, kujundaja Marje Eelmaa (Tuumik Stuudio). Maastikuarhitektuuri aastapreemia 2016 "Maastikuarhitektuuri tegu" laureaat

  11. Ot rokoko do romantizma / Jevgeni Ashihmin

    Index Scriptorium Estoniae

    Ashihmin, Jevgeni

    2006-01-01

    Suveekskursioonide tsüklist "Eesti kunsti ajaloost" Kumus. Ekskursioonid teemadel "Rokokoost romantismini","Modernism. II Maailmasõja eelne eesti kunst" ja "Eesti kunst nõukogude perioodil". Ekskursioone juhivad kunstiteadlased Helena Risthein ja Karin Taidre

  12. Алексий II 1929-2008

    Index Scriptorium Estoniae

    2008-01-01

    Aleksius II meenutavad: Kornelius, Tallinna ja kogu Eesti metropoliit ; Karin Jaani ; Marina Kaljurand ; Dmitri Medvedev, Venemaa president ; Helene Kamzol, Aleksius II sugulane ; Ain Seppik ; Vladimir Illjashevitsh ; Edgar Savisaar ; Jevgeni Tomberg ; Ene Ergma ; Toomas Hendrik Ilves, Eesti Vabariigi president

  13. Mereteemaline festival Melbourneìs / Sirje Jõgi

    Index Scriptorium Estoniae

    Jõgi, Sirje

    2006-01-01

    oktoobrikuu viimasel pühapäeval Soome majas, eestlaste ühendkoor Karin Adamsoni juhatusel avas ühisfestivali. "Kirjud Vestid" esitasid Taevo Vaikma loodud laulu, "Eiderattad" esinesid kolme tantsuga

  14. Nostalgia Arhitektuurimuuseumis : kuldsed kuuekümnendad / Karen Jagodin ; interv. Ellu Maar

    Index Scriptorium Estoniae

    Jagodin, Karen, 1982-

    2007-01-01

    Näitusest "Kuuekümnendad. Eesti moodne arhitektuur" ja sisearhitektide Väino Tamme ning Vello Asi loomingu näitusest Eesti Arhitektuurimuuseumis. Kuraatorid Karin Hallas-Murula ja Karen Jagodin. Eesti 1960. aastate sisearhitektuurist

  15. Oliivipuude triloogia - mõnus suvine võrkkiige-lugemine / Mari Klein

    Index Scriptorium Estoniae

    Klein, Mari

    2007-01-01

    Tutvustus: Drinkwater, Carol. Oliiviistandus / tlk. Laine Hone. Tallinn : Varrak, 2004 ; Drinkwater, Carol. Oliivisaak / tlk. Eve-Reet Tammet. Tallinn : Varrak, 2997 ; Drinkwater, Carol. Oliivihooaeg / tlk. Lane Hone ja Karin Rita. Tallinn : Varrak, 2006

  16. Rotermanni soolalao juurde rajatakse urbanistlik park / Kertu Kalmus

    Index Scriptorium Estoniae

    Kalmus, Kertu

    2008-01-01

    Eesti Arhitektuurimuuseumi korraldatud Rotermanni soolalao esise urbanistliku pargi võistluse võitis Villem Tomiste töö "Piazza". Žürii esimees Eesti Arhitektuurimuuseumi direktor Karin Hallas-Murula

  17. Raamatureisid Veneetsiasse / Margus Haav

    Index Scriptorium Estoniae

    Haav, Margus, 1969-

    2005-01-01

    Arvustus: Girardi, Robert. Vaporetto 13 / ingl. k. tlk. Karin Suursalu. Tallinn : Kultuurileht, 2005. (Loomingu Raamatukogu) ; Raittila, Hannu. Canal Grande : [romaan] / tõlkinud Aivo Lõhmus. Tallinn : Varrak, 2003 (Moodne aeg)

  18. Saku uus logo liidab traditsioonid ja uuendusmeelsuse

    Index Scriptorium Estoniae

    2007-01-01

    Reklaamiagentuur Divisioni loometööd juhtinud Dan Mikkin Saku Õlletehase uue logo loomisprotsessist ja stiiliraamatu kokkupanemisest. Karin Sepa kommentaar "Uus logo on avatum". Illustratsiooniks Saku õlletehase logod läbi aegade

  19. Kirgas klaas / Margit Aedla

    Index Scriptorium Estoniae

    Aedla, Margit, 1970-

    2008-01-01

    Klaasiga saab ruumi tuua kergust ja värvilise klaasiga lisada pilkupüüdvat aktsenti. Valik klaasist riiuleid (disainerid Aziz Sariyer, Karin Rashid, Jean Nouvel), laevalgusti (disainer Michiko Nakata), seinakell, diivanilaud

  20. "Impact 4" Berliinis ja Poznanis / Vappu Thurlow

    Index Scriptorium Estoniae

    Thurlow, Vappu, 1960-

    2005-01-01

    5.-10. IX Berliinis ja Poznanis toimunud neljandast rahvusvahelisest graafikakonverentsist "Impact" alapealkirjaga "Kontakt". Eestist osalesid Loit Jõekalda, Eve Kask, Karin Laansoo ja Vappu Thurlow. 2007. a. Tallinnas toimuva "Impact 5", alapealkirjaga "Slices of Time/Lõigutud aeg", kontseptsioon

  1. 75 FR 76482 - Federal Housing Administration (FHA): FHA Maximum Loan Limits for 2011

    Science.gov (United States)

    2010-12-08

    ... http://www.hud.gov/offices/adm/hudclips/letters/mortgagee/ . FOR FURTHER INFORMATION CONTACT: Karin B... be found at http://www.hud.gov/offices/adm/hudclips/letters/mortgagee/ mortgagee/. Dated: November 29...

  2. ATM-dialoog Arne Maasiku ainetel / Andreas Trossek, Aleksander Tsapov

    Index Scriptorium Estoniae

    Trossek, Andreas, 1980-

    2008-01-01

    Kuni 17. II Tallinna Kunstihoones avatud Arne Maasiku fotode näitusest (kuraator Karin Paulus, kujundaja Urmas Luure) ja fotoalbumist "Fotod : 1994-2007" (tekstide autorid Eero Epner, Peeter Laurits, Jan Kaus, kujundaja Martin Pedanik)

  3. About Nation, Race, and Ethnicity in Latin America in the 19th and 20th centuries

    Directory of Open Access Journals (Sweden)

    Hanni Jalil Paier

    2013-12-01

    Full Text Available Bibliographic outline of the following book:APPELBAUM, Nancy P., Anne S. Macpherson and Karin A. Rosemblatt (Eds. (2003. Race and Nation in Modern Latin America. Chapel Hill: University of North Carolina Press, 329 pp.

  4. BENCHMARKING - PRACTICAL TOOLS IDENTIFY KEY SUCCESS FACTORS

    Directory of Open Access Journals (Sweden)

    Olga Ju. Malinina

    2016-01-01

    Full Text Available The article gives a practical example of the application of benchmarking techniques. The object of study selected fashion store Company «HLB & M Hennes & Mauritz», located in the shopping center «Gallery», Krasnodar. Hennes & Mauritz. The purpose of this article is to identify the best ways to develop a fashionable brand clothing store Hennes & Mauritz on the basis of benchmarking techniques. On the basis of conducted market research is a comparative analysis of the data from different perspectives. The result of the author’s study is a generalization of the ndings, the development of the key success factors that will allow to plan a successful trading activities in the future, based on the best experience of competitors.

  5. Decision-making framework for testing and grouping of nanomaterials

    OpenAIRE

    Landsiedel, Robert

    2015-01-01

    A ‘multiple perspective’ framework for the grouping of nanomaterials Robert Landsiedel presenting the results of the ECETOC Nano Task Force (Josje H.E. Arts a, Mackenzie Hadi b, Athena M. Keene c, Reinhard Kreiling d, Delina Lyon e, Monika Maier f, Karin Michel g, Thomas Petry h, Ursula G. Sauer i, David Warheit j, Karin Wiench k, Robert Landsiedel k) a AkzoNobel, Technology and Engineering, Arnhem, Netherlands b Shell Health, Shell International B.V., The Hague, Netherlands...

  6. The Nuclear Death Domain Protein p84N5; A Candidate Breast Cancer Susceptibility Gene

    Science.gov (United States)

    2007-05-01

    hallmarks of cancer. Cell 100, 57-70, 2000. 35. Karin M, Cao Y, Greten FR, Li ZW. NF-kappaB in cancer: from innocent bystander to major culprit. Nat Rev...D., and Weinberg, R. A. (2000) Cell 100, 57-70 46. Karin, M., Cao, Y., Greten , F. R., and Li, Z. W. (2002) Nat Rev Cancer 2, 301-310 47. Cogswell, P

  7. Ideed, mis muudavad maailma

    Index Scriptorium Estoniae

    2012-01-01

    Noorte ettevõtluskonkursi Ajujaht finalistidest: Richard Murutar (Dolphin), Andrus Purde (Achoo), Kalev Külaase, Ülane Vilumets, Rait Kapp (Kohalik Giid), Mart Raus, Vahur Mäe (Grillcube), Dmitri Kuznetsov, Anna Agronova, Julia Abolina, Anatoly Loginov (Healthiest.mobi), Karin Juhe, Karin Aruots (Korsid), Raino Sinisalu, Märt Pikkani, Arkadi Tammik, Varje Papp (Raybike), Egle Loit, Kair Käsper (Pille toidukott)

  8. Nano Materials

    Science.gov (United States)

    2007-03-01

    2006 Nanodiamonds applications • Lapping and polishing: – Memory Disk – Eyeglass Lenses – Miniature and Precision Ball Bearings – Orthopedic...lubricate by rolling like miniature ball bearings 41 Nanotechnology lectures: Henne van Heeren, enablingMNT, nanomaterials, November 2006 MoS2

  9. Primary Squamous Cell Carcinoma of Stomach: A Rare Entity ...

    African Journals Online (AJOL)

    Schmidt C, Schmid A, Lüttges JE, Kremer B, Henne-Bruns D. Primary squamous cell carcinoma of the stomach. Report of a case and review of literature. Hepatogastroenterology 2001;48:1033-6. 5. Muto M, Hasebe T, Muro K, Boku N, Ohtsu A, Fujii T, et al. Primary squamous cell carcinoma of the stomach: A case report with ...

  10. Short Outlines of Books by Estonian Authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2009-01-01

    Arvustus: Kaplinski, Jaan. Parallelle ja parallelisme. Tartu : Tartu Ülikooli Kirjastus, c2009 ; Hellat, Henn-Kaarel. Inimese tegemine I-V. Tallinn : Faatum, 2002-2008 ; Nõu, Helga. Elu täis üllatusi. Tartu : Atlex, 2009 ; Remsu, Olev. Musketäride muundumised. [Tallinn] : Eesti Keele Sihtasutus, 2008 ; Ristikivi, Karl. Päevaraamat 1957-1968. Tallinn : Varrak, 2008

  11. Eesti Kirjanike Liit praegu : ankeedivastused

    Index Scriptorium Estoniae

    1997-01-01

    Aut.: Ain Kaalep, Juhan Habicht, Valeria Ränik, Karl Muru, Mart Velsker, Janika Kronberg, Maie Kalda, Hannes Varblane, Toomas Raudam, Priidu Beier, Kati Murutar, Dagmar Normet, Enn Vetemaa, Henn-Kaarel Hellat, Vello Lattik, Kajar Pruul, Kauksi Ülle, Ene Mihkelson, Jüri Ehlvest

  12. Uus ja uhke campus valmib aastaks 2010

    Index Scriptorium Estoniae

    2006-01-01

    Tallinna Tehnikaülikooli haldusdirektor Henn Karits tutvustab ülikooli lähimate aastate ehitusplaane - peamaja rekonstrueerimist, majandus- ja humanitaarteaduskondade hoone ning raamatukogu uue hoone ehitamist. Uus campus sisaldab endas ka maa-aluse parkla, spordikompleksi, üliõpilasühiselamud

  13. Manhattanilt Tartu tahahoovi / Vaapo Vaher

    Index Scriptorium Estoniae

    Vaher, Vaapo, 1945-

    2008-01-01

    Tutvustus: Dos Passos, John. Manhattan Transfer / tlk. Olavi Teppan. Tallinn : Tänapäev, 2007 ; Betti Alver : usutlused. Kirjad. Päevikukatked. Mälestused / koostanud ja toimetanud Kristi Metste, Enn Lillemets. Tallinn : Tänapäev, 2007 ; Artur Alliksaar mälestustes / koostanud Henn-Kaarel Hellat. Tartu : Ilmamaa, 2007. Ka kirjastusest Ilmamaa

  14. Development of COS-SNP and HRM markers for cost efficient and reliable haplotype-based detection of Lr14a in durum wheat (Triticum durum Desf.)

    Science.gov (United States)

    Leaf rust (Puccinia triticina Eriks. & Henn.) is a major disease affecting durum wheat production. The Lr14a leaf rust resistant gene present in the durum wheat cv. Creso and its derivative Colosseo is one of the best characterized leaf rust resistance sources presently deployed in durum wheat breed...

  15. Kilingi-Nõmme on miljööväärtuslik väikelinn / Tõnu Kann

    Index Scriptorium Estoniae

    Kann, Tõnu, 1957-

    2009-01-01

    Tõnu Kanni ja Henn Soodla kuues matk Pärnumaa 20. sajandi väärtarhitektuuri jälgedes, kaasas kunstiteadlane Epp Lankots, kes leidis Kilingi-Nõmmel kuus hoonet, mida ta pakub riikliku kultuurimälestisena kaitse alla võtta. Üks neist Konstantin Meyeri projekti järgi ehitatud funktsionalistlik villa

  16. Ääremaal võib kohata ilusaid maalähedasi hooneid / Tõnu Kann

    Index Scriptorium Estoniae

    Kann, Tõnu, 1957-

    2009-01-01

    Tõnu Kanni ja Henn Soodla matk Pärnumaa 20. sajandi väärtarhitektuuri jälgedes, kaasas kunstiteadlase Epp Lankotsi koostatud Eesti 20. sajandi arhitektuuri kaitseprogrammi nimestik kaitsealustest objektidest Urissaares, Massiarus, Metsapooles ja Võidukülas, viimases uudismaa-asunduste hooned projekteeris Erika Nõva, Massiaru koolimaja autor oli Märt Merivälja

  17. Streigi saatus selgub täna lõunaks / Eno-Gerrit Link

    Index Scriptorium Estoniae

    Link, Eno-Gerrit

    2007-01-01

    Riiklik lepitaja Henn Pärn tegi viimase kompromissettepaneku. Juhul, kui ametiühingute, tööandjate ja lepitaja kohtumisel osalised seda heaks ei kiida, alustatakse hoiatusstreigiga. Vt. samas: Pärnu streikivate bussijuhtide pöördumine linnarahva poole

  18. Minu tänav / Anne Metsis

    Index Scriptorium Estoniae

    Metsis, Anne, 1958-

    2016-01-01

    Vahva nimega kodutänavatest. Vestlusest Tallinna Ülikooli tervisedenduse õppejõu Hele Leek-Amburiga, kes elab Haapsalus Neidude tänaval, moekunstnik Anu Stranbergiga Pääskülas Päikese puiesteelt ja akordionist Henn Rebasega Viimsis Mesipuu teelt

  19. Genome-wide association study for Identification and validation of novel SNP markers for Sr6 stem rust resistance gene in bread wheat

    Science.gov (United States)

    Stem rust (caused by Puccinia graminis f. sp. tritici Erikss. & E. Henn.), is a major disease in wheat (Triticum aestivium L.). However, in recent years it occurs rarely in Nebraska due to weather and the effective selection and gene pyramiding of resistance genes. To understand the genetic basis of...

  20. Raamat väikerahvaste terminoloogiast / Tiina Leemets

    Index Scriptorium Estoniae

    Leemets, Tiina

    1994-01-01

    Rahvusvahelise terminoloogiainstituudi IITF-i väljaande "Terminology Science and Research" väikekeelte terminoloogia olukorrale pühendatud erinumbrist (nr. 2/1991), kus käsitletakse eesti (Tiiu Erelt, Henn Saari), läti, leedu, ukraina, lapi, islandi, mänksi, iiri, katalaani ja baski oskuskeelt

  1. "Kepi mind" sai külge pornosildi / Tiit Tuumalu

    Index Scriptorium Estoniae

    Tuumalu, Tiit, 1971-

    2001-01-01

    Prantsuse mängufilmi "Kepi mind" ("Baise-moi"), mida Filmimax näitab juba kuu aega "Sõpruse" kinos, tunnistas kultuuriministeeriumi juures tegutsev ekspertkomisjon, mida juhtis Henn Vallimäe, pornograafiat ja vägivalda sisaldavaks ning seega alaealistele keelatuks

  2. Nanotechnology and Lifestyle

    Science.gov (United States)

    2006-11-01

    nano & lifestyle, November 2006 12 Bodywarmers etc (Invista, Outlast Technologies, ToastyFeet) • ToastyFeet/Aspen Aerogel : 5% sheets of fiber...submarines and aircraft ) and B/C-warfare agent decontamination (tent materials and tarpaulins). Surfaces Nato lectures, Henne van Heeren, enablingMNT, nano

  3. Sequencing of an Anthracnose-resistant sorghum genotype and mapping of a major QTL reveal strong candidate genes for Anthracnose resistance

    Science.gov (United States)

    Anthracnose, caused by the fungal pathogen Colletotrichum sublineolum Henn. ex. Sacc. and Trotter 1913, is an economically damaging disease of sorghum [Sorghum bicolor (L.) Moench] in hot and humid production regions of the world. Control of anthracnose is almost exclusively through the use of genet...

  4. Effect of fungicide on the development of wheat stem rust and yield ...

    African Journals Online (AJOL)

    Stem rust caused by Puccinia graminis f.sp tritici Erik. & E. Henn. is a highly destructive disease of wheat (Triticum aestivum L.). The effects of fungicide application on stem rust (Puccinia graminis tritici) epidemics and yield of three bread wheat varieties varying in reaction to the disease were studied in two major wheat ...

  5. Ilo Pariisi varjus / Kaia Sisask

    Index Scriptorium Estoniae

    Sisask, Kaia

    2003-01-01

    Portreefilm Pariisis giidina töötavast kirjanik Juhan Jaigi tütrest Ilo Jaik-Riedbergist "Ilo Pariisis" : stsenarist ja režissöör Peep Puks : operaatorid Arvo Vilu, Ago Ruus, Heli Arvo Vilu, Henn Liiva, Jüri Vaher : teksti loeb Anu Lamp : muusikaline kujundus Merike Vaino : filmi direktor Maie Kerma : Pro-Film 2002

  6. Vedelkütusevaru on põhjendatud erand / Alan Vaht

    Index Scriptorium Estoniae

    Vaht, Alan, 1976-

    2005-01-01

    Vastus Henn Pärna artiklile "Vedelkütusevaru tuleb soetada kindlasti riigihankega" 17. märtsi Äripäevas. Majandus- ja kommunikatsiooniministeerium on veendunud, et kõik vedelkütusevarude moodustamisega seotud riigihanked teostati kooskõlas Eesti seadustega

  7. Tallinna-Tartu liinil jääb käima üks rong / Hindrek Riikoja

    Index Scriptorium Estoniae

    Riikoja, Hindrek

    2001-01-01

    Teede- ja sideminister Toivo Jürgenson ning Edelaraudtee juhatuse esimees Henn Ruubel allkirjastasid reisijateveo lepingu, mille järgi jääb Tallinna-Tartu liinil käima üks rong päevas. Uued bussiliinid

  8. Discovery of a novel stem rust resistance allele in durum wheat that exhibits differential reactions to Ug99 isolates

    Science.gov (United States)

    Wheat stem rust, caused by Puccinia graminis f. sp. tritici Erikss. & E. Henn, can incur yield losses on susceptible cultivars of durum wheat, Triticum turgidum ssp. durum (Desf.) Husnot. Though several durum cultivars possess the stem rust resistance gene Sr13, additional genes in durum wheat effec...

  9. Identification and validation of single nucleotide polymorphic markers linked to Ug99 stem rust resistance in spring wheat

    Science.gov (United States)

    Wheat stem rust (Puccinia graminis f. sp. tritici Eriks. and E. Henn.) is one of the most destructive diseases world-wide. Races belonging to Ug99 (or TTKSK) continue to cause crop losses in East Africa and threaten global wheat production. Developing and deploying wheat varieties with multiple race...

  10. Genetic mapping of stem rust resistance to Puccinia graminis f. sp. tritici race TRTTF in the Canadian wheat cultivar 'Harvest'

    Science.gov (United States)

    Stem rust, caused by Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn.(Pgt), is a destructive disease of wheat that can be controlled by deploying effective stem rust resistance (Sr) genes. Highly virulent races of Pgt in Africa have been detected and characterized. These include race T...

  11. Sources of stem rust resistance in Ethiopian tetraploid wheat ...

    African Journals Online (AJOL)

    Stem or black rust of wheat caused by the fungus Puccinia graminis f. sp. tritici Ericks and Henn (Pgt) is an important disease on wheat worldwide. Pgt is an obligate biotroph, heteroceous in its life cycle and heterothallic in mating type. Seedlings of 41 emmer (Triticum dicoccum), 56 durum (T. durum) wheat accessions were ...

  12. Molecular and cytogenetic characterization of wheat introgression lines carrying the stem rust resistance gene Sr39.

    Science.gov (United States)

    Stem rust, caused by Puccinia graminis Pers.:Pers. f.sp. tritici Eriks. and Henn., poses a serious threat to global wheat production because of the emergence of Pgt-TTKSK (Ug99). The TTKSK resistant gene Sr39 was derived from Aegilops speltoides through chromosome translocation. In this study, we ch...

  13. Wheat stem rust in South Africa: Current status and future research ...

    African Journals Online (AJOL)

    . In South Africa, stem rust caused by Puccinia graminis Pers. f. sp. tritici. Eriks. & E. Henn. (Pgt) is an important disease of wheat. Records of stem rust occurrence in South Africa date back to the late 1720's, when it was first discovered in the ...

  14. Judgment of the Constitutional Review Chamber of the Supreme Court en banc : no. of the case 3-4-1-5-02 : date of desicion 28 October 2002

    Index Scriptorium Estoniae

    2002-01-01

    Riigikohtu lahendi 3-4-1-5-02 (Tallinna Halduskohtu taotlus ORAS §7 lg 3 põhiseadusele vastavuse selgitamiseks) tekst inglise keeles. - Lisatud: Dissenting opinion by Jüri Ilvest, Henn Jõks, Lea Kivi, Villu Kõve, pp. 214-215

  15. Suur Mast teeb väikest Skype'i / Madis Kaal ; interv. Merlis Nõgene

    Index Scriptorium Estoniae

    Kaal, Madis

    2006-01-01

    Infotehnoloogiaettevõtte Skype töötaja räägib oma haridusteest, karjäärist ja perekonnast, ettevõtte töökorraldusest, saavutustest ja tulevikuplaanidest. Kommenteerib sõber ja kolleeg Henn Ruukel

  16. Single residue AAV capsid mutation improves transduction of photoreceptors in the Abca4-/- mouse and bipolar cells in the rd1 mouse and human retina ex vivo.

    Science.gov (United States)

    De Silva, Samantha R; Charbel Issa, Peter; Singh, Mandeep S; Lipinski, Daniel M; Barnea-Cramer, Alona O; Walker, Nathan J; Barnard, Alun R; Hankins, Mark W; MacLaren, Robert E

    2016-11-01

    Gene therapy using adeno-associated viral (AAV) vectors for the treatment of retinal degenerations has shown safety and efficacy in clinical trials. However, very high levels of vector expression may be necessary for the treatment of conditions such as Stargardt disease where a dual vector approach is potentially needed, or in optogenetic strategies for end-stage degeneration in order to achieve maximal light sensitivity. In this study, we assessed two vectors with single capsid mutations, rAAV2/2(Y444F) and rAAV2/8(Y733F) in their ability to transduce retina in the Abca4 -/- and rd1 mouse models of retinal degeneration. We noted significantly increased photoreceptor transduction using rAAV2/8(Y733F) in the Abca4 -/- mouse, in contrast to previous work where vectors tested in this model have shown low levels of photoreceptor transduction. Bipolar cell transduction was achieved following subretinal delivery of both vectors in the rd1 mouse, and via intravitreal delivery of rAAV2/2(Y444F). The successful use of rAAV2/8(Y733F) to target bipolar cells was further validated on human tissue using an ex vivo culture system of retinal explants. Capsid mutant AAV vectors transduce human retinal cells and may be particularly suited to treat retinal degenerations in which high levels of transgene expression are required.

  17. Inflammation and Immune Response of Intra-Articular Serotype 2 Adeno-Associated Virus or Adenovirus Vectors in a Large Animal Model

    Directory of Open Access Journals (Sweden)

    Akikazu Ishihara

    2012-01-01

    Full Text Available Intra-articular gene therapy has potential for the treatment of osteoarthritis and rheumatoid arthritis. To quantify in vitro relative gene transduction, equine chondrocytes and synovial cells were treated with adenovirus vectors (Ad, serotype 2 adeno-associated virus vectors (rAAV2, or self-complementary (sc AAV2 vectors carrying green fluorescent protein (GFP. Using 6 horses, bilateral metacarpophalangeal joints were injected with Ad, rAAV2, or scAAV2 vectors carrying GFP genes to assess the in vivo joint inflammation and neutralizing antibody (NAb titer in serum and joint fluid. In vitro, the greater transduction efficiency and sustained gene expression were achieved by scAAV2 compared to rAAV2 in equine chondrocytes and synovial cells. In vivo, AAV2 demonstrated less joint inflammation than Ad, but similar NAb titer. The scAAV2 vectors can induce superior gene transduction than rAAV2 in articular cells, and both rAAV2 and scAAV2 vectors were showed to be safer for intra-articular use than Ad vectors.

  18. Pharmacology of Recombinant Adeno-associated Virus Production

    Directory of Open Access Journals (Sweden)

    Magalie Penaud-Budloo

    2018-03-01

    Full Text Available Recombinant adeno-associated viral (rAAV vectors have been used in more than 150 clinical trials with a good safety profile and significant clinical benefit in many genetic diseases. In addition, due to their ability to infect non-dividing and dividing cells and to serve as efficient substrate for homologous recombination, rAAVs are being used as a tool for gene-editing approaches. However, manufacturing of these vectors at high quantities and fulfilling current good manufacturing practices (GMP is still a challenge, and several technological platforms are competing for this niche. Herein, we will describe the most commonly used upstream methods to produce rAAVs, paying particular attention to the starting materials (input used in each platform and which related impurities can be expected in final products (output. The most commonly found impurities in rAAV stocks include defective particles (i.e., AAV capsids that do contain the therapeutic gene or are not infectious, residual proteins from host cells and helper viruses (adenovirus, herpes simplex virus, or baculoviruses, and illegitimate DNA from plasmids, cells, or helper viruses that may be encapsidated into rAAV particles. Given the role that impurities may play in immunotoxicity, this article reviews the impurities inherently associated with each manufacturing platform.

  19. Induction of immunity to antigens expressed by recombinant adeno-associated virus depends on the route of administration.

    Science.gov (United States)

    Brockstedt, D G; Podsakoff, G M; Fong, L; Kurtzman, G; Mueller-Ruchholtz, W; Engleman, E G

    1999-07-01

    Recombinant adeno-associated virus (rAAV) is a replication-defective parvovirus which is being explored as a vector for gene therapy because of its broad host range, excellent safety profile, and durable transgene expression in infected hosts. rAAV has also been reported by several groups to induce little or no immune response to its encoded transgene products. In this study we examined the immunogenicity of rAAV by studying the immune response of C57BL/6 mice to a single dose of rAAV-encoding ovalbumin (AAV-Ova) administered by a variety of routes. Mice injected with AAV-Ova intraperitoneally (ip), intravenously, or subcutaneously developed potent ovalbumin-specific cytotoxic T lymphocytes (CTL) as well as anti-ovalbumin antibodies and antibodies to AAV. In contrast, mice injected with AAV-Ova intramuscularly developed a humoral response to the virus and the transgene but minimal ovalbumin-specific CTLs. The induced CTL response after ip administration of AAV-Ova protected mice against a subsequent tumor challenge with an ovalbumin-transfected B16 melanoma cell line. Studies of the mechanism by which AAV-Ova induces CTL confirmed that the virus delivers the transgene product into the classical MHC class I pathway of antigen processing. Mice that previously had been exposed to rAAV vectors failed to develop ovalbumin-specific CTL following administration of AAV-Ova. Analysis of these mice revealed the presence of circulating anti-AAV antibodies that blocked rAAV transduction in vitro and inhibited CTL induction in vivo. These results suggest a possible role for rAAV in the immunotherapy of malignancies and viral infections, although induced antibody responses to AAV may limit its ability to be administered for repeated vaccinations. Copyright 1999 Academic Press.

  20. Autorongiga teises riigis põhjustatud liikluskahju: millise riigi õiguse järgi hinnatakse ühe kindlustusandja võimalikku tagasinõuet teise kindlustusandja vastu? Euroopa Kohtu otsus liidetud kohtuasjades C-359/14 ja C-475/14 / Karin Sein, Andru

    Index Scriptorium Estoniae

    Sein, Karin, 1974-

    2017-01-01

    Euroopa Kohtu kohtulahenditest nr. C-359/14 ja C-475/14. Veduki ja haagise omaniku tsiviilvastutusest Saksa õiguses. Kindlustusandjate tagasinõudeõigusest veduki ja haagise Eestis kindlustamise korral

  1. 1920. aastate Eesti-Soome arhitektuurisuhetest ja Soome arhitektide töödest Pärnu supelasutuse konkursil = Suomen ja Viron arkkitehtuurin suhteista 1920-luvulla ja suomalaisarkkitehtien ehdotuksista Pärnun kylpylän kilpailussa / Karin Hallas-Mur

    Index Scriptorium Estoniae

    Hallas-Murula, Karin, 1957-

    2004-01-01

    1923. a. avati Helsingis Into Saxeli ja Johan Sigfrid Sireni kavandatud mälestusmärk Eesti Vabadussõjas osalenud Soome vabatahtlikele. Monumendi konkursist. Soome arhitektide osalemisest 1923. a. Tallinna tehnikumi (I - Martti Välikangas, Hilding Ekelund) ning 1922. a. ja 1925. a. Pärnu supelasutuse konkurssidel (tuntumad osalejad Uno Ullberg, Alvar Aalto, H. Ekelund, Pauli Ernesti Blomstedt, Ragnar Ypyä, edukamad osalejad Georg Henriksson ja Otto Flodin) jm. Pikemalt A. Aalto projektist. Bibliograafia joonealustes märkustes

  2. Teatri aastaauhindade nominendid 2006. aasta loomingu eest

    Index Scriptorium Estoniae

    2007-01-01

    Nimekiri nominentidest teatri aastaauhinnale sõna-, muusika-, balleti-, tantsulavastuste ja teatri tehnilise töötaja auhinna kategoorias. Kunstnikuauhind: Vladimir Anshon - "Nii see on (kui teile nii näib)" (Tallinna Linnateater) ja "Valged ööd" (Theatrum), Andris Freibergs - "Karin. Indrek. Tõde ja õigus. 4." (Tallinna Linnateater), Kristine Pasternaka - kostüümid lavastustele "Karin. Indrek. Tõde ja õigus. 4." ja "Nii see on (kui teile nii näib" (Tallinna Linnateater), Ene-Liis Semper - "Kuningas Ubu" (NO99), Ervin Õunapuu -"Sada aastat..." (Vanemuine).

  3. Recombinant AAV-mediated in vivo long-term expression and antitumour activity of an anti-ganglioside GM3(Neu5Gc) antibody.

    Science.gov (United States)

    Piperno, G M; López-Requena, A; Predonzani, A; Dorvignit, D; Labrada, M; Zentilin, L; Burrone, O R; Cesco-Gaspere, M

    2015-12-01

    The ganglioside GM3(Neu5Gc) has gained increasing attention as therapeutic target because of its selective expression in various human tumours, such as melanoma, breast and lung cancer. 14F7 is a mouse IgG1 with specific reactivity to GM3(Neu5Gc)-positive tumours. The therapeutic activity of 14F7 has also been demonstrated in vivo, through its repetitive passive administration in tumour-bearing animals. In this work we used an alternative strategy to deliver recombinant 14F7 in vivo and analysed the therapeutic efficacy of this approach. We engineered a recombinant adeno-associated vector to direct the expression of secretable recombinant 14F7 in BALB/c animals. A single administration of the rAAV induced efficient production and secretion of the antibody in the bloodstream, with an expression level reaching plateau at ∼3 weeks after injection and persisting for almost a year. Strikingly, upon challenge with GM3(Neu5Gc)-positive X63-AG8.653 myeloma cells, tumour development was significantly delayed in animals treated with rAAV-14F7 with respect to animals treated with a control rAAV codifying for an irrelevant antibody. Finally, no significant differences in survival proportion were detected in animals injected with rAAV-14F7 or treated by standard administration of repetitive doses of purified monoclonal antibody 14F7.

  4. Palga tõusuks jääb raha puudu / Airi Amtmann

    Index Scriptorium Estoniae

    Amtmann, Airi

    2008-01-01

    Paljud Raplamaa omavalitsused on probleemi ees, kuidas kindlustada pedagoogidele 22%-list palgatõusu. Arvamust avaldavad Vigala vallavanem Priit Kärsna, Käru vallavanem Elari Hiis, Rapla abivallavanem Imbi Kalberg, Kohila vallavanem Hillar Kalmet, Juuru vallavalitsuse haridus- ja kultuurispetsialist Aili Normak ja haridusministeeriumi nõunik Riho Raave

  5. AAV-Mediated Gene Targeting Is Significantly Enhanced by Transient Inhibition of Nonhomologous End Joining or the Proteasome In Vivo

    Science.gov (United States)

    Paulk, Nicole K.; Loza, Laura Marquez; Finegold, Milton J.

    2012-01-01

    Abstract Recombinant adeno-associated virus (rAAV) vectors have clear potential for use in gene targeting but low correction efficiencies remain the primary drawback. One approach to enhancing efficiency is a block of undesired repair pathways like nonhomologous end joining (NHEJ) to promote the use of homologous recombination. The natural product vanillin acts as a potent inhibitor of NHEJ by inhibiting DNA-dependent protein kinase (DNA-PK). Using a homology containing rAAV vector, we previously demonstrated in vivo gene repair frequencies of up to 0.1% in a model of liver disease hereditary tyrosinemia type I. To increase targeting frequencies, we administered vanillin in combination with rAAV. Gene targeting frequencies increased up to 10-fold over AAV alone, approaching 1%. Fah−/−Ku70−/− double knockout mice also had increased gene repair frequencies, genetically confirming the beneficial effects of blocking NHEJ. A second strategy, transient proteasomal inhibition, also increased gene-targeting frequencies but was not additive to NHEJ inhibition. This study establishes the benefit of transient NHEJ inhibition with vanillin, or proteasome blockage with bortezomib, for increasing hepatic gene targeting with rAAV. Functional metabolic correction of a clinically relevant disease model was demonstrated and provided evidence for the feasibility of gene targeting as a therapeutic strategy. PMID:22486314

  6. Soojalt sojast / Ülle Hõbemägi

    Index Scriptorium Estoniae

    Hõbemägi, Ülle

    2008-01-01

    Sojast, sojatoodetest ning sojatoitudest räägivad aroomterapeut ja massöör Marge Teder, Soya Foods Baltic OÜ müügidirektor Meeli Matsalu, tudeng ja taimetoitlane Loore-Emilie Raav ning Angelica Udeküll

  7. Assessment of tropism and effectiveness of new primate-derived hybrid recombinant AAV serotypes in the mouse and primate retina.

    Directory of Open Access Journals (Sweden)

    Peter Charbel Issa

    Full Text Available Adeno-associated viral vectors (AAV have been shown to be safe in the treatment of retinal degenerations in clinical trials. Thus, improving the efficiency of viral gene delivery has become increasingly important to increase the success of clinical trials. In this study, structural domains of different rAAV serotypes isolated from primate brain were combined to create novel hybrid recombinant AAV serotypes, rAAV2/rec2 and rAAV2/rec3. The efficacy of these novel serotypes were assessed in wild type mice and in two models of retinal degeneration (the Abca4(-/- mouse which is a model for Stargardt disease and in the Pde6b(rd1/rd1 mouse in vivo, in primate tissue ex-vivo, and in the human-derived SH-SY5Y cell line, using an identical AAV2 expression cassette. We show that these novel hybrid serotypes can transduce retinal tissue in mice and primates efficiently, although no more than AAV2/2 and rAAV2/5 serotypes. Transduction efficiency appeared lower in the Abca4(-/- mouse compared to wild type with all vectors tested, suggesting an effect of specific retinal diseases on the efficiency of gene delivery. Shuffling of AAV capsid domains may have clinical applications for patients who develop T-cell immune responses following AAV gene therapy, as specific peptide antigen sequences could be substituted using this technique prior to vector re-treatments.

  8. Assessment of tropism and effectiveness of new primate-derived hybrid recombinant AAV serotypes in the mouse and primate retina.

    Science.gov (United States)

    Charbel Issa, Peter; De Silva, Samantha R; Lipinski, Daniel M; Singh, Mandeep S; Mouravlev, Alexandre; You, Qisheng; Barnard, Alun R; Hankins, Mark W; During, Matthew J; Maclaren, Robert E

    2013-01-01

    Adeno-associated viral vectors (AAV) have been shown to be safe in the treatment of retinal degenerations in clinical trials. Thus, improving the efficiency of viral gene delivery has become increasingly important to increase the success of clinical trials. In this study, structural domains of different rAAV serotypes isolated from primate brain were combined to create novel hybrid recombinant AAV serotypes, rAAV2/rec2 and rAAV2/rec3. The efficacy of these novel serotypes were assessed in wild type mice and in two models of retinal degeneration (the Abca4(-/-) mouse which is a model for Stargardt disease and in the Pde6b(rd1/rd1) mouse) in vivo, in primate tissue ex-vivo, and in the human-derived SH-SY5Y cell line, using an identical AAV2 expression cassette. We show that these novel hybrid serotypes can transduce retinal tissue in mice and primates efficiently, although no more than AAV2/2 and rAAV2/5 serotypes. Transduction efficiency appeared lower in the Abca4(-/-) mouse compared to wild type with all vectors tested, suggesting an effect of specific retinal diseases on the efficiency of gene delivery. Shuffling of AAV capsid domains may have clinical applications for patients who develop T-cell immune responses following AAV gene therapy, as specific peptide antigen sequences could be substituted using this technique prior to vector re-treatments.

  9. Iglesiase-Anett sai eluajal Kolga-Jaani ausamba / Rainer Kerge

    Index Scriptorium Estoniae

    Kerge, Rainer, 1977-

    2008-01-01

    ETV "Erisaate" eestvedamisel püstitati Kolga-Jaani ausammas tühisusele. Ausammas kujutab Anett Arnimit, kes oli esikohal Eesti Ekspressi koostatud tühiste kuulsuste edetabelis. Kuju valmistasid skulptuuritudengid Loore-Emilie Raav, Regina Kuningas ja Kadri Allekand

  10. Jalutuskäik Tallinna galeriides laupäeval, 17. XII / Juta Kivimäe

    Index Scriptorium Estoniae

    Kivimäe, Juta, 1952-

    2005-01-01

    Jaan Elkeni ja Anu Põdra ühisnäitus "Mina ja tema" Deco galeriis kuni 30. XII. Kristin Kalamehe näitus "Mehed" Tallinna Linnagaleriis kuni 1. I 2006. Alice Kase joonistused Hobusepea galeriis 13.-19. XII. Rita Raave näitus "Vana sein" G-galeriis kuni 31. XII

  11. Kunstitudengid näitavad Maarjamäe lossi pargis oma vabadussamba tõlgendusi

    Index Scriptorium Estoniae

    2008-01-01

    Eesti Kunstiakadeemia skulptuuritudengid Marit Mardla, Matthias-Jakob Sildnik, Mikk Madisson, Liis Dvorjanski, Uku Sepisvart, Hannes Aasamets, Kadri Allekand, Kristin Orav, Loore Raav, Jekaterina Kultajeva, Li Yang, Kristel Aasjõe, Liivi Tantaal, Jass Kaselaan, Edith Karlson, Kadri Metstak, Berit Talpsepp ja Eike Eplik näitavad oma töid näitusel "Vabaduse monumendid"

  12. Recombinant Adeno-Associated Virus-Mediated microRNA Delivery into the Postnatal Mouse Brain Reveals a Role for miR-134 in Dendritogenesis in Vivo

    DEFF Research Database (Denmark)

    Christensen, Mette; Larsen, Lars A; Kauppinen, Sakari

    2010-01-01

    delivery of microRNAs in vivo by use of recombinant adeno-associated virus (rAAV). rAAV-mediated overexpression of miR-134 in neurons of the postnatal mouse brain provided evidence for a negative role of miR-134 in dendritic arborization of cortical layer V pyramidal neurons in vivo, thereby confirming...

  13. Adeno-associated viral vector serotypes 1 and 5 targeted to the neonatal rat and pig striatum induce widespread transgene expression in the forebrain

    DEFF Research Database (Denmark)

    Kornum, Birgitte R; Stott, Simon R W; Mattsson, Bengt

    2010-01-01

    Viral vector-mediated gene transfer has emerged as a powerful means to target transgene expression in the central nervous system. Here we characterized the efficacy of serotypes 1 and 5 recombinant adeno-associated virus (rAAV) vectors encoding green fluorescent protein (GFP) after stereotaxic...

  14. Biological effects of rAAV-caAlk2 coating on structural allograft healing

    DEFF Research Database (Denmark)

    Koefoed, Mette; Ito, Hiromu; Gromov, Kirill

    2005-01-01

    Structural bone allografts often fracture due to their lack of osteogenic and remodeling potential. To overcome these limitations, we utilized allografts coated with recombinant adeno-associated virus (rAAV) that mediate in vivo gene transfer. Using beta-galactosidase as a reporter gene, we show...

  15. Tallinna Visioonikonverents 2010 : Linn ja linnakultuur 22. sajandil

    Index Scriptorium Estoniae

    2010-01-01

    Tallinna Lauluväljaku ruumides 22. novembril toimunud Tallinna Visioonikonverentsist "Linn ja linnakultuur 22. sajandil", kus arutleti teemal, milline võiks olla Tallinn saja aasta pärast. Konverentsil esinesid Toomas Vitsut, Mart Saarma, Jan Sturesson, Raivo Raave, Satish Kumar, Juhani Pallasmaa ning moderaatoriteks olid Hardo Aasmäe ja Mati Heidmets

  16. Adeno-associated viral vector serotypes 1 and 5 targeted to the neonatal rat and pig striatum induce widespread transgene expression in the forebrain

    DEFF Research Database (Denmark)

    Kornum, Birgitte R; Stott, Simon R W; Mattsson, Bengt

    2010-01-01

    . Our results show that striatal delivery of rAAV5 vectors in the neonatal brain represents a useful tool to express genes of interest both in the basal ganglia and the neocortex. Furthermore, we apply, for the first time, viral vector-mediated gene transfer to the pig brain providing the opportunity...

  17. Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences

    Directory of Open Access Journals (Sweden)

    Dilley Robert

    2009-01-01

    Full Text Available Abstract Background Adeno-associated virus (AAV vectors are promising tools for gene therapy. Currently, their potential is limited by difficulties in producing high vector yields with which to generate transgene protein product. AAV vector production depends in part upon the replication (Rep proteins required for viral replication. We tested the hypothesis that mutations in the start codon and upstream regulatory elements of Rep78/68 in AAV helper plasmids can regulate recombinant AAV (rAAV vector production. We further tested whether the resulting rAAV vector preparation augments the production of the potentially therapeutic transgene, insulin-like growth factor I (IGF-I. Results We constructed a series of AAV helper plasmids containing different Rep78/68 start codon in combination with different gene regulatory sequences. rAAV vectors carrying the human IGF-I gene were prepared with these vectors and the vector preparations used to transduce HT1080 target cells. We found that the substitution of ATG by ACG in the Rep78/68 start codon in an AAV helper plasmid (pAAV-RC eliminated Rep78/68 translation, rAAV and IGF-I production. Replacement of the heterologous sequence upstream of Rep78/68 in pAAV-RC with the AAV2 endogenous p5 promoter restored translational activity to the ACG mutant, and restored rAAV and IGF-I production. Insertion of the AAV2 p19 promoter sequence into pAAV-RC in front of the heterologous sequence also enabled ACG to function as a start codon for Rep78/68 translation. The data further indicate that the function of the AAV helper construct (pAAV-RC, that is in current widespread use for rAAV production, may be improved by replacement of its AAV2 unrelated heterologous sequence with the native AAV2 p5 promoter. Conclusion Taken together, the data demonstrate an interplay between the start codon and upstream regulatory sequences in the regulation of Rep78/68 and indicate that selective mutations in Rep78/68 regulatory elements

  18. Hongos tremeloides (Heterobasidiomycetes de la Reserva de la Biosfera de Calakmul, Campeche, México Tremelloid fungi (Heterobasidiomycetes from Calakmul Biosphere Reserve, Campeche, Mexico

    Directory of Open Access Journals (Sweden)

    Sigfrido Sierra

    2012-03-01

    Full Text Available Se registran 7 especies de hongos tremeloides de la Reserva de la Biosfera de Calakmul: Auricularia cornea Ehrenb., A. delicata (Fr. Henn., A. mesenterica (Dicks. Pers., Dacryopinax elegans (Berk. et M.A. Curtis G.W. Martin, D. spathularia (Schwein. G.W. Martin, Tremella wrightii Berk. et M.A. Curtis y Tremelloscypha gelatinosa (Murrill Oberw. et K. Wells. Todas son registros nuevos para la reserva. Auricularia cornea y T. gelatinosa son nuevos registros para el estado de Campeche.Seven species of tremelloid fungi are recorded from Calakmul Biosphere Reserve: Auricularia cornea Ehrenb., A. delicata (Fr. Henn., A. mesenterica (Dicks. Pers., Dacryopinax elegans (Berk. et M.A. Curtis G.W. Martin, D. spathularia (Schwein. G.W. Martin, Tremella wrightii Berk. et M.A. Curtis and Tremelloscypha gelatinosa (Murrill Oberw. et K. Wells. All are new records for the reserve. Auricularia cornea and T. gelatinosa are new records for Campeche state.

  19. Vratshi i medsestrõ... za bortom peregovorov / Anna Litvinjuk

    Index Scriptorium Estoniae

    Litvinjuk, Anna

    2007-01-01

    Eesti Arstide Liit ja Eesti Keskastme Tervishoiutöötajate Kutseliit ei kiida heaks lepingut, mis kehtib vaid Haiglate Liidu liikmetele ja jätab palgatõusust ilma terve rea tervishoiutöötajaid. Tervishoiutöötajad pöörduvad kohtusse. Vt. samas: Kokkuvõtteks; Kommenteerivad: Eesti Arstide Liit, sotsiaalminister Jaak Aab, riiklik lepitaja Henn Pärn

  20. Short outlines of books by Estonian authors : [annotations] / Rutt Hinrikus, Janika Kronberg

    Index Scriptorium Estoniae

    Hinrikus, Rutt, 1946-

    1999-01-01

    Madis Kõiv. Kähri ker'ko man Pekril; Hannes Varblane. Avalikult halb : valik; Andrus Kivirähk. Liblikas; Juhan Paju. Vanaema mõis; Henn Mikelsaar. Ristiratast; Asta Põldmäe. Viini plika; Ellen Niit. Paekivi laul; Toomas Raudam. Elus enesetapja; Andrus Kivirähk. Pagari piparkook; Oskar Kruus. Hella Wuolijoki; Hasso Krull. Jazz : nelikümmend luuletust; Mart Kivastik. Varblane; Jüri Ehlvest. Elli lend

  1. Libatse "vabaõhumuuseumist" Pärnu-Jaagupisse / Tõnu Kann

    Index Scriptorium Estoniae

    Kann, Tõnu, 1957-

    2009-01-01

    Tõnu Kanni ja Henn Soodla seitsmes matk Pärnumaa 20. sajandi väärtarhitektuuri jälgedes, kaasas kunstiteadlane Epp Lankots, kes peab Libatset kompaktseks, hästi planeeritud mitmekesise hoonetüpoloogiaga asulaks koos nägusa haljastusega - tüüpprojektide "Kullipesa" ja "Ella" arhitektid on Tõnu Kull ja Ülevi Eljand, kaupluse projekteerija - Olga Bruns

  2. Bussijuhtide kindrali rasked päevad / Peep Peterson ; interv. Tanel Raig

    Index Scriptorium Estoniae

    Peterson, Peep, 1975-

    2007-01-01

    Eesti Transpordi- ja Teetöötajate Ametiühingu esimees Peep Peterson vastab bussijuhtide ja tööandjate vahelisi lahkhelisid, ametiühinguliikumist ning kokkuleppe sõlmimist puudutavatele küsimustele. Kommenteerivad: Villem Tori. Streikija iseloomuga raske vastane; Henn Pärn. Peterson saavutas maksimumide maksimumi; Harri Taliga. Ametiühingu liidritel erinevad arusaamad. Lisa: Fakte eluloost

  3. Les plantes tinctoriales d'Afrique Centrale : enquête ethnobotanique ...

    African Journals Online (AJOL)

    SARAH

    30 juil. 2013 ... naturels n'a pas été suffisamment exploré. Le domaine reste encore non valorisé, malgré l'utilisation traditionnelle de nombreuses plantes pour leurs propriétés colorantes : Curcuma longa pour la cuisine, Lawsonia inermis (Henné) ou. Pterocarpus soyauxii pour la cosmétique,. Indigofera tinctoria pour les ...

  4. Reproductive output of mosses under experimental warming on Fildes Peninsula, King George Island, maritime Antarctica

    OpenAIRE

    Casanova-Katny, A.; Torres-Mellado, G. A.; Eppley, S. M.

    2016-01-01

    BACKGROUND: Mosses dominate much of the vegetation in the Antarctic, but the effect of climatic change on moss growth and sexual reproduction has scarcely been studied. In Antarctica, mosses infrequently produce sporophytes; whether this is due to physiological limitation or an adaptive response is unknown. We studied the effect of experimental warming (with Open Top Chambers, OTCs) on sporophyte production on Fildes Peninsula, King George Island for four moss species (Bartramia patens, Henne...

  5. Eesti proosa 1994 : [ka Noor-Eesti fenomenist 1994. a eesti proosas] / Toomas Liiv

    Index Scriptorium Estoniae

    Liiv, Toomas, 1946-2009

    1995-01-01

    Kangro, Bernard. Härjanädalate aegu; Kõiv, Madis Rännuaastad. Studia memoriae. I; Vanapa, Andres. Riiulid; Mutt, Mihkel. Rahvusvaheline mees; Berg, Maimu. Ma armastasin venelast. Nemad; Kross, Jaan. Järelehüüd; Kudu, Reet. Vabadus ja armastus; Mikelsaar, Henn. Käiski Nuustakul ära; Mihkelson, Ene. Nime vaev; Pukk, Holger. Suvest suveni; Trubetsky, Tõnu & Anti Pathique. Daam sinises; Traat, Mats. Kodu on ilus; Traat, Mats. Minge üles mägedele. Teine raamat, jt.

  6. IT ei loe / Nicholas G. Carr

    Index Scriptorium Estoniae

    Carr, Nicholas G.

    2003-01-01

    Harvard Business Review endise toimetaja arvates on infotehnoloogia (IT) strateegiline tähtsus ettevõtetes oluliselt vähenenud ning innovaatilised IT lahendused ei anna enam konkurentsieeliseid, mille tõttu peab muutuma ka juhtide suhtumine IT riskidesse, investeeringutesse ja juhtimisse. Vt. samas: Kuidas vältida üleinvesteerimist IT-sse?; Liiga palju head; Mis saab müüjatest?; Uued reeglid IT juhtimises. Kommenteerivad Henn Sarv, Avo Kokk, Olli Heinonen ja Jüri Kaljundi

  7. Festival 'Sünnisõnad' ئ igaühele midagi / Evi Arujärv

    Index Scriptorium Estoniae

    Arujärv, Evi, 1953-

    2000-01-01

    29. dets. 1999 - 1. jaan. 2000 toimunud festivalist "Sünnisõnad". Lühidalt ka nonstopfilmist 'Sajand arhitektuuri. Elavad pildid' (autor Karin Hallas), arhitektuurimuuseumi näitusest 'Eesti katedraalid'. 30. XII Nyyd Ensemble esitatud Gavin Bryarsi teosest 'Titanicu hukk' (Jaan Toomiku videod) jm.

  8. An Anthropology of Learning in Epistemic Cultures

    DEFF Research Database (Denmark)

    Hasse, Cathrine

    2015-01-01

    I connect Karin Knorr-Cetina's concept of ‘epistemic cultures’ with an anthropological conceptualization of practice-based learning. The theory of practice-based learning I explore departs from the cultural psychologist Lev Vygotsky’s notion of word-meaning which can be seen as a basic unit...

  9. The End of the Six-Party Talks?

    Science.gov (United States)

    2007-01-01

    Pyongyang is wary of Beijing’s international influence, its ability to collaborate with the United States and South Korea, its willingness to foster ...Kenneth Quinones, “ Dualism in the Bush Administration’s North Korea Policy,” Asian Perspective, XXVII, no. 1, 2003, 197-224; Karin Lee and Adam Miles

  10. Grunge kodukujunduses / Ene Vool

    Index Scriptorium Estoniae

    Vool, Ene, 1948-

    2004-01-01

    Low-tech, campi ja grunge ehitus- ja sisustusstiilidest meil ja mujal - kommenteerivad Andro Kööp ja Ain Nurmela. Tutvustatakse ka Mari-Mira projekti Pariisis, mis kujutab endast nn. mutant-küla, putka- ja onnikultuuri koketeriid. Artikli lõpus kommenteerib kunstiteadlane Karin Paulus grungestiili muusikas, riietuses ja sisekujunduses.

  11. Adoptive T cell cancer therapy

    Science.gov (United States)

    Dzhandzhugazyan, Karine N.; Guldberg, Per; Kirkin, Alexei F.

    2018-06-01

    Tumour heterogeneity and off-target toxicity are current challenges of cancer immunotherapy. Karine Dzhandzhugazyan, Per Guldberg and Alexei Kirkin discuss how epigenetic induction of tumour antigens in antigen-presenting cells may form the basis for multi-target therapies.

  12. Baltic beer market : close neighbours, different situations

    Index Scriptorium Estoniae

    2004-01-01

    Baltimaade juhtivate õlletootjate marketingijuhid Martins Dzenis (Cesu Alus), Inita Drezina (Aldaris), Eriks Kalita (Livu Alus), Raimonds Kalnins (Kimmel), Dalius Rutkauskas (AB Ragutis), Dainius Smailys (Svyturis-Utenos Alus), Katrin Vernik (A. Le Coq Eesti), Karin Sepp (Saku Õlletehas) ja Härmo Põlluvee (Frederik Viru Õlu) olukorrast Baltimaade õlleturul, suurematest tarbijamängudest ja reklaamikampaaniatest. Tabelid

  13. Browse Title Index

    African Journals Online (AJOL)

    Items 1 - 25 of 1853 ... (Cnidaria: Actiniaria) a mobile sea anemone attacking octocorals, Abstract PDF. Karin Riemann-Zürneck, Charles L. Griffiths. Vol 35, No 2 (2000), Kroyeria deetsi n.sp. (Kroyeriidae: Siphonostomatoida), a parasitic copepod infecting gills of spinner sharks, Carcharhinus brevipinna (Müller & Henle, 1839), ...

  14. African Zoology - Vol 34, No 4 (1999)

    African Journals Online (AJOL)

    Korsaranthus natalensis (Carlgren, 1938) nov. comb. (Cnidaria: Actiniaria) a mobile sea anemone attacking octocorals · EMAIL FREE FULL TEXT EMAIL FREE FULL TEXT DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. Karin Riemann-Zürneck, Charles L. Griffiths, 190-196 ...

  15. TiSSA pleenum "Sotsiaaltöö kriisi ajal" : mida on sotsiaaltööl pakkuda ja kes saab sellest kasu? / Regina Lind

    Index Scriptorium Estoniae

    Lind, Regina

    2010-01-01

    22.-27. aug. 2010 toimus Tallinna Ülikoolis TiSSA doktorantide eelkonverents ja pleenum. Pleenumil peetud sotsiaalteadlaste ettekannetest. Esinesid: Holger Ziegler, Karin Böllert, Catrin Heite, Hans van Ewijk, Walter Lorenz, Mikko Mäntysaari, Heinz Sünker, Synnöve Karvinen-Niinikoski, Olga Borodkina

  16. Sõda, viin, naised ja surm / Vaapo Vaher

    Index Scriptorium Estoniae

    Vaher, Vaapo, 1945-

    2005-01-01

    Tutvustus: Gelassimov, Andrei. Janu / vene k. tlk. Jaan Ross. Tallinn : Kultuurileht, 2005. (Loomingu Raamatukogu) ; Austen, Jane. Mõistus ja tunded / inglise k. tlk. Karin Suursalu. Tallinn : Tänapäev, 2005 ; Kiik, Heino. Ole usin : 1948-1954. Tartu : Ilmamaa, 2005 ; Palm, August. Villem Reiman : [1861-1917]. 2., täiend. tr. Tartu : Ilmamaa, 2004

  17. Kulka aastapreemiate žürii hinnang = Comments from the panel of judges / Laila Põdra, Eva Hirvesoo, Tiit Sild, Epp Lankots ; intervjueerinud Margit Mutso

    Index Scriptorium Estoniae

    2011-01-01

    Arhitektuuriaastast 2010. Eesti Kultuurkapitali aastapreemia - Maarja Kask, Karli Luik, Ralf Lõoke, Kristiina Arusoo, Margit Argus, Pelle-Sten Viiburg (Sõmeru keskusehoone, maanteemuuseumi välialad). Arhitektuuri sihtkapitali arhitektuuripreemia - Hannes Koppel (Kuressaare rannahoone), Raul Vaiksoo (eramu), restaureerimispreemia - Jaan Jõgi (Laupa mõis), sisearhitektuuri preemia - Leila Pärtelpoeg (Röa mõis), tegevuspreemia - Karin Hallas-Murula, Eesti Arhitektuurikeskus

  18. Kattuvad hääled / Reet Varblane

    Index Scriptorium Estoniae

    Varblane, Reet, 1952-

    2008-01-01

    Iisraeli ja palestiina kunstnike näitus "Kattuvad hääled/Overlapping Voices" Essli muuseumis Klosterneuburgis Austrias 16. V-25. X. Kuraatorid: Tal Adler (Iisrael, juut), Amal Murkus (Iisrael, palestiinlane), Karin Schneider ja Friedemann Derschmidt (Austria). Karlheinz ja Agnes Esslile kuuluvast eramuuseumist (1991, arhitekt Heinz Tesar)

  19. 77 FR 36285 - Notice of Proposed Information Collection: Comment Request; Application for Fee or Roster...

    Science.gov (United States)

    2012-06-18

    ... the FHA insurance fund. Agency form numbers, if applicable: HUD-92563I, HUD 92563A, HUD 92564-CN... to the proposal by name and/or OMB Control Number and should be sent to: Reports Liaison Officer... number for the Federal Information Relay Service (1-800-877-8339). FOR FURTHER INFORMATION CONTACT: Karin...

  20. Moodsad näidisasumid : Weissenhof & Maasika-Vaarika : näitus Eesti Arhitektuurimuuseumis 21. 04.-20. 05. 2001 / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    2001-01-01

    Karin Kirschi ja Tiina Tammeti koostatud näituse põhjal 1927. a. Stuttgarti rajatud Weissenhofi väikemajade elamurajoonist (arhitektid Walter Gropius, Bruno Taut, Le Corbusier jt.) ja 1931-1932 Tallinnasse ehitatud Maasika-Vaarika tänava asumit

  1. Raznõje litsa Indreka Paasa / Eteri Kekelidze

    Index Scriptorium Estoniae

    Kekelidze, Eteri, 1944-

    2008-01-01

    Vargamäel 22 tundi kestnud maratonetendusest Tammsaare "Tõe ja õiguse" põhjal - Elmo Nüganeni lavastused "Tõde ja õigus. Teine osa", "Karin. Indrek. Tõde ja õigus. 4" ja "Wargamäe Wabariik" (III osa) ning Jaanus Rohumaa lavastus "Vargamäe kuningriik" (I ja V osa). Pikemalt "Wargamäe Wabariigi" lavastusest

  2. 8. III esitleti Adamson-Ericu muuseumis ajakirja Muuseum 2005. aasta esimest numbrit

    Index Scriptorium Estoniae

    2005-01-01

    Tomislav Shola kirjutab "Kvaliteedist pärimusinstitutsioonides". Rahvusvaheliste konverentside kogemusi jagavad Toomas Abiline, Aino Lepp, Lea Sillart ja Karin Hallas-Murula. Eesti muuseumide olulistest tähtpäevadest kirjutavad Urmas Dresen, Mari-Ann Aimla, Marika Valk ja Merike Koppel

  3. Neueste Forschung in musiktherapeutischer Diagnostik

    DEFF Research Database (Denmark)

    Ridder, Hanne Mette Ochsner

    2012-01-01

    responsivem Verhalten. In der Musiktherapie sind mehrere Assessmentmodelle entwickelt worden, unter anderem der IAP von Kenneth Bruscia, AQR von Karin Schumacher und Claudine Calvet-Kruppa oder das Assessment von Kontaktniveaus bei Anne Steen. Nach dieser Einführung des Assessmentbegriffes werden zusätzlich...

  4. Raamat / Matis Rodin

    Index Scriptorium Estoniae

    Rodin, Matis

    2012-01-01

    Raamatututvustus: Paulus, Karin. Tootedisain : asjad minu elus. Tallinn : Eesti Kunstiakadeemia, 2011; Vainküla, Tõnu, Pere, René. Vana mööbel korda oma kätega. [Tallinn] : Ajakirjade Kirjastus, 2012; Ruumipilt 2011 : Eesti parimad interjöörid ja disain / Eesti Sisearhitektide Liit ; toimetaja ja vastutav väljaandja Priit Põldme. Tallinn, 2012

  5. Tulumaks taas kähku riigi kätte : avansiline tulumaks seadusest kadumas / Lemmi Kann

    Index Scriptorium Estoniae

    Kann, Lemmi

    2008-01-01

    Dividendide avansiline maksustamine võib määramata ajaks edasi lükkuda. Lisatud: kehtiv kord sunnib tulumaksu kohe maksma ; KPMG Baltics ASi maksuvaldkonna juhi Joel Zernaski ja Harju Elektri finantsdirektori Karin Padjuse kommentaarid: maksudega ei maksa mängida

  6. 1 küsimus noorele juhile : Kuidas peab keskjuht otsused töötajateni viima? / Andrus Soodla, Mairi Jüriska, Terje Pihl... [jt.

    Index Scriptorium Estoniae

    2009-01-01

    Küsimusele vastavad Danske Bank AS-i kliendisuhete juht Andrus Soodla, SA Terve Eesti juht Mairi Jüriska, reklaamiagentuuri Tank töötaja Terje Pihl, audiitor- ja konsultatsioonifirma KPMG siseauditi teenusliini juht Karin Rätsep, konsultatsiooni- ja audiitorfirma Ernst & Young ärikonsultatsioonide osakonna juhataja Tarmo Toiger ning Andrese Klaasi ja Dekoori tegevjuht Gerd Veelma

  7. Leisure in a Multicultural Society

    NARCIS (Netherlands)

    Peters, K.B.M.

    2008-01-01

    Leisure practices and public space, second chapter. Karin Peters discusses ethnic diversity and leisure from three perspectives. She first looks at the leisure patterns in terms of ethnic participation. Based on theories of marginality and ethnicity, she argues that both socio-economic and

  8. Dying for Pain Relief (A Cup of Health with CDC)

    Centers for Disease Control (CDC) Podcasts

    2013-07-04

    It’s an increasingly common story— a woman is found dead in her home from a prescription pain killer overdose. In this podcast, Dr. Karin Mack discusses the growing problem of pain killer overdoses among women.  Created: 7/4/2013 by MMWR.   Date Released: 7/4/2013.

  9. Astronaut C. Michael Foale is briefed on use of Sky Genie

    Science.gov (United States)

    1994-01-01

    Astronaut C. Michael Foale, STS-63 mission specialist, is briefed on the use of Sky Genie device by Karin L. Porter. The device would aid in emergency egress operations aboard a troubled Space Shuttle. Porter, an employee of Rockwell International, helps train astronauts in egress procedures at JSC's Shuttle mockup and integration laboratory.

  10. Küberkiusaja käib varjuna lapse kannul / Sigrid Kõiv

    Index Scriptorium Estoniae

    Kõiv, Sigrid

    2008-01-01

    Üle kolmandiku õpilastest on netikiusamist kogenud ja enam kui veerand õpilastest on ise selles osalenud, näitab Tartu Ülikooli magistrandi Karin Naruskovi uurmistöö. Kiusajate kõige menukamateks vahenditeks on MSN ja Rate. Kommenteerib IBM Eesti juht Valdo Randpere

  11. Puitarhitektuur ja puit arhitektuuris / Piret Lindpere

    Index Scriptorium Estoniae

    Lindpere, Piret, 1963-

    1999-01-01

    Näitus "Eesti puitarhitektuur" Rotermanni soolalaos 12. septembrini. Koostajad Karin Hallas (stiiliajalugu) ja Epi Tohvri (väikelinnad). Kujundaja Ralf Tamm. Puitarhitektuuri säilitamisest Tallinnas ja väikelinnades. Puidu arhitektuuris kasutamise näide ئ 1998. a. valminud Linnamäe kool Läänemaal (arhitekt Tiit Trummal).

  12. Book review : Street Architecture: Work by Hans van der Heijden

    NARCIS (Netherlands)

    Jenniskens, D.P.H.

    The recently published book Street Architecture is the result of a collaboration between Karin Templin, currently pursuing a PhD in architecture at the University of Cambridge, and Hans van der Heijden, an Amsterdam-based architect of mainly urban renewal projects and residential architecture. The

  13. 75 FR 8677 - Seamless Refined Copper Pipe and Tube From the People's Republic of China and Mexico...

    Science.gov (United States)

    2010-02-25

    ... Copper Pipe and Tube From the People's Republic of China and Mexico: Postponement of Preliminary...: Karine Gziryan (the People's Republic of China) or Joy Zhang (Mexico), AD/CVD Operations, Offices 4 and 3... Tube from the People's Republic of China and Mexico. See Seamless Refined Copper Pipe and Tube from the...

  14. Eesti Draamateater tähistas maja 100. sünnipäeva / Tiiu Laks

    Index Scriptorium Estoniae

    Laks, Tiiu, 1984-

    2010-01-01

    18. septembril tähistati avatud uste päevaga Eesti Draamateater hoone 100. sünnipäeva. Õhtul sai teatrihoone fassaadilt vaadata Taavet Janseni, Taavi Varmi ja Andres Tenusaare videoinstallatsiooni. Esitleti raamatut "Eesti Draamateatri maja 100" (autorid Karin Hallas-Murula, Kristel Pappel, Haldja Jalajas). Avati Peeter Lauritsa fotonäitus "Pahempidi"

  15. South African Journal of Plant and Soil: Editorial Policies

    African Journals Online (AJOL)

    Journal Management: Combined Congress Continuing Committee. Karin Hannweg, SASHS President, ARC–Institute for Tropical and Sub-Tropical Crops, Nelspruit, South Africa Arno Janse van Vuuren, SASCP President, NWK Bpk, Lichtenburg, South Africa Piet le Roux, SSSSA Vice-President, University of the Free State, ...

  16. Rahvusvaheline erialabibliograafia sündis Eestis / Piret Voolaid

    Index Scriptorium Estoniae

    Voolaid, Piret, 1971-

    2005-01-01

    Rets. rmt.: Internationale volkskundliche Bibliographie = International Folklore Bibliographie = Bibliographie internationale d'Ethnologie: Für das Jahr 1993 : mit Nachträgen für die vorausgehenden / herausgegeben von Karin Maria Rooleid. Bonn : Dr. Rudolf Habelt GMBH, 2004

  17. Vargamäelt leigust ei leitud / Jan Kaus

    Index Scriptorium Estoniae

    Kaus, Jan, 1971-

    2008-01-01

    Vargamäel 21. juunil 22 tundi kestnud maratonetendusest Tammsaare "Tõe ja õiguse" põhjal - Elmo Nüganeni lavastused "Tõde ja õigus. Teine osa", "Karin. Indrek. Tõde ja õigus. 4" ja "Wargamäe Wabariik" (III osa) ning Jaanus Rohumaa lavastus "Vargamäe kuningriik" (I ja V osa)

  18. Nii hea, kui keegi mõtleb absoluudi peale

    Index Scriptorium Estoniae

    2008-01-01

    11. I avatakse Tallinna Kunstihoones Arne Maasiku fotode näitus. Kuraator Karin Paulus, kujundaja Urmas Luure. Detsembris 2007 ilmus Urmas Luure monograafia, fotod 1994-2007, kujundaja Martin Pedanik. Lõigud Eero Epneri, Jan Kausi ja Peeter Lauritsa sissejuhatavatest kirjutistest

  19. Urmas Ott versus Pille-Riin Purje / Andry Ervald

    Index Scriptorium Estoniae

    Ervald, Andry

    2007-01-01

    Urmas Oti intervjuuraamat väliseesti ajakirjaniku, luuletaja ja prosaisti Karin Saarseniga "Grand Hotel, Stockholm. Poola suvi" : SE&JS Kirjastus, 2006 ja Pille-Riin Purje raadiosaate "Hiirelõks" tarvis intervjuudest kokku pandud vestluste kogumik "Kuraditosin näitlejat hiirelõksus" : Varrak, 2007

  20. Like-Me Simulation as an Effective and Cognitively Plausible Basis for Social Robotics

    Science.gov (United States)

    2009-02-24

    havior of teams from observation. In: RoboCup 2002: robot soccer world cup, Fukuoka, Japan 25. Karin-D’Arcy MR, Povinelli DJ (2002) Do chimpanzees...2001) Building a multimodal human-robot interface. IEEE Intel Syst 16:16–20 41. Povinelli DJ, Eddy TJ (1994) The eyes as a window: what young

  1. Angels and Demons in the Book of Jubilees

    OpenAIRE

    Ruiten, Jacques van; Reiterer, F.V.; Nicklas, T.; Schöpflin, K.

    2007-01-01

    Jacques van Ruiten, “Angels and Demons in the Book of Jubilees,” in Angels: The Concept of Celestial Beings – Origins, Development and Reception (ed. Friedrich V. Reiterer, Tobias Nicklas, and Karin Schöpflin; Deuterocanonical and Cognate Literature Yearbook 2007; Berlin and New York: de Gruyter, 2007), 585-609.

  2. Angels and Demons in the Book of Jubilees

    NARCIS (Netherlands)

    Ruiten, Jacques van; Reiterer, F.V.; Nicklas, T.; Schöpflin, K.

    2007-01-01

    Jacques van Ruiten, “Angels and Demons in the Book of Jubilees,” in Angels: The Concept of Celestial Beings – Origins, Development and Reception (ed. Friedrich V. Reiterer, Tobias Nicklas, and Karin Schöpflin; Deuterocanonical and Cognate Literature Yearbook 2007; Berlin and New York: de Gruyter,

  3. Endogenous and exogenous factors in national development ...

    African Journals Online (AJOL)

    radio stations saw his performance where he was adjudged the winner at the ... influence. This is consistent with Karin Barber's position that popular culture be read not ... of the writer apparently colour the poem in the figurative language of a political type, ..... It is always constructed through memory, fantasy, narrative and.

  4. Groener drogen

    NARCIS (Netherlands)

    Loman, N.G.M. (Niek)

    2012-01-01

    Auteur: N.G.M. (Niek) Loman Begeleiders: Ing. H.W. (Pim) van den Dool, Discrete Technologie en Productie Automatisering, RUG Dr.ir. I. (Ingrid) ten Have MBA, Interfac. Opleidingsinst. Technische Bedrijfskunde, RUG Drs. C.M. (Karin) Ree, Bèta Wetenschapswinkel, Science & Society Group, RUG Drs. R.W.

  5. Universal jurisdiction: state of affairs and ways ahead. : A policy paper

    NARCIS (Netherlands)

    L. Zegveld (Liesbeth); J.D. Handmaker (Jeff)

    2012-01-01

    textabstractOn 17 September 2010, the International Institute of Social Studies (ISS), Leiden University and ICCO organised an expert meeting at the ISS on universal jurisdiction (UJ). The meeting was chaired in the morning by Professor John Dugard and in the afternoon by Professor Karin Arts. The

  6. Beyond Racism and Poverty : The Truck System on Louisiana Plantations and Dutch peateries, 1865-1920

    NARCIS (Netherlands)

    Lurvink, K.

    The truck system was a global phenomenon in the period 1865-1920, where workers were paid through the company store. In Beyond Racism and Poverty Karin Lurvink looks at how this system functioned on plantations in Louisiana in comparison with peateries in the Netherlands. In the United States, the

  7. Industrial variographic analysis for continuous sampling system validation

    DEFF Research Database (Denmark)

    Engström, Karin; Esbensen, Kim Harry

    2017-01-01

    Karin Engström, LKAB mining, Kiruna, Sweden, continues to present illuminative cases from process industry. Here she reveals more from her ongoing PhD project showing application of variographic characterisation for on-line continuous control of process sampling systems, including the one...

  8. Plaadid / Heidi Purga

    Index Scriptorium Estoniae

    Purga, Heidy, 1975-

    2006-01-01

    Uutest heliplaatidest Jay Dee/J Dilla "Donuts", The Strokes "First Impressions Of Earth", Jools Holland & His R&B orchestra "Swinging the Blues, Dancing The Ska", "The Sound Of Music", Karin Clercq "Apres l'amour", Mest "Photographs", The Notorious B.I.G. "Duets"

  9. "Tõde ja õigus" ilmutab end prantslastele : [vestlusring A. H. Tammsaare peateose tõlkijatega] / Jean Pascal Ollivry, Eva Toulouze, Jean-Pierre Minaudier ; vestlust juhtinud Tiina Väljaste

    Index Scriptorium Estoniae

    Ollivry, Jean Pascal

    2010-01-01

    2009. a. ilmus kirjastus Gaïa väljaandel "Tõde ja õigus" kolm esimest köidet: "La colline-du-voleur", "Indrek" (mõlemad tõlkis J. P. Ollivry) ja "Jours d'emeutes" (tlk. J.-P. Minaudier). E. Toulouze tõlgib romaani 4. osa ("Indrek et Karin") ning J. P. Ollivry 5. osa

  10. Hiigelämblik kanali kohal ehk Kuidas ERMi uus hoone lõi kired lõkkele / Tarmo Michelson

    Index Scriptorium Estoniae

    Michelson, Tarmo, 1975-

    2006-01-01

    Eesti Rahva muuseumi uue hoone arhitektuurikonkursi võidutööst "Memory Field" (autorid itaalia arhitekt Dan Dorell, liibanoni arhitekt Lina Ghotmeh, jaapani arhitekt Tsuyoshi Tane). Kommenteerivad Jaanus Plaat, Tõnis Lukas, Karin Hallas-Murula, Raivo Palmaru, Laine Jänes, Marika Valk

  11. Dying for Pain Relief (A Cup of Health with CDC)

    Centers for Disease Control (CDC) Podcasts

    It’s an increasingly common story— a woman is found dead in her home from a prescription pain killer overdose. In this podcast, Dr. Karin Mack discusses the growing problem of pain killer overdoses among women.

  12. 77 FR 65361 - Xanthan Gum From Austria and the People's Republic of China: Postponement of Preliminary...

    Science.gov (United States)

    2012-10-26

    ... Austria and the People's Republic of China: Postponement of Preliminary Determinations of Antidumping Duty...: October 26, 2012. FOR FURTHER INFORMATION CONTACT: Karine Gziryan (Austria) or Brandon Farlander (People's... of initiation of antidumping duty investigations of xanthan gum from Austria and the People's...

  13. Installation Service - Changes in opening hours

    CERN Multimedia

    GS Department

    2010-01-01

    For organizational matters, please note that, as from 15 March 2010, the Installation Service will have wen opening hours. The new schedule will be from 14:00 to 17:00 (Monday to Friday). Contact persons are: Martine Briant, Karine Robert and Claudia Bruggmann. The office address remains 73-3-014. Installation Service

  14. Linnapea, keda rõõmustab titekisa / Jüri Ratas ; interv. Riina Luik

    Index Scriptorium Estoniae

    Ratas, Jüri, 1978-

    2006-01-01

    Tallinna linnapea vastab küsimustele, mis puudutavad tema suhtumist opositsiooni kriitikasse, linna juhtimist, perekonda, lapsepõlve, spordiga tegelemist, sõpruskonda, eraettevõtlusest loobumist ning suhteid kirikuga. Lisa: Jüri Ratas (27). Vt. samas lühiintervjuud linnapea abikaasa Karin Ratasega. Kommenteerib Jüri Ratase endine klassijuhataja Nõmme gümnaasiumist, võrkpallitreener Raimund Pundi

  15. Intrathecal long-term gene expression by self-complementary adeno-associated virus type 1 suitable for chronic pain studies in rats

    Directory of Open Access Journals (Sweden)

    Janssen William GM

    2006-01-01

    Full Text Available Abstract Background Intrathecal (IT gene transfer is an attractive approach for targeting spinal mechanisms of nociception but the duration of gene expression achieved by reported methods is short (up to two weeks impairing their utility in the chronic pain setting. The overall goal of this study was to develop IT gene transfer yielding true long-term transgene expression defined as ≥ 3 mo following a single vector administration. We defined "IT" administration as atraumatic injection into the lumbar cerebrospinal fluid (CSF modeling a lumbar puncture. Our studies focused on recombinant adeno-associated virus (rAAV, one of the most promising vector types for clinical use. Results Conventional single stranded rAAV2 vectors performed poorly after IT delivery in rats. Pseudotyping of rAAV with capsids of serotypes 1, 3, and 5 was tested alone or in combination with a modification of the inverted terminal repeat. The former alters vector tropism and the latter allows packaging of self-complementary rAAV (sc-rAAV vectors. Combining both types of modification led to the identification of sc-rAAV2/l as a vector that performed superiorly in the IT space. IT delivery of 3 × 10e9 sc-rAAV2/l particles per animal led to stable expression of enhanced green fluorescent protein (EGFP for ≥ 3 mo detectable by Western blotting, quantitative PCR, and in a blinded study by confocal microscopy. Expression was strongest in the cauda equina and the lower sections of the spinal cord and only minimal in the forebrain. Microscopic examination of the SC fixed in situ with intact nerve roots and meninges revealed strong EGFP fluorescence in the nerve roots. Conclusion sc-rAAVl mediates stable IT transgene expression for ≥ 3 mo. Our findings support the underlying hypothesis that IT target cells for gene transfer lack the machinery for efficient conversion of the single-stranded rAAV genome into double-stranded DNA and favor uptake of serotype 1 vectors over 2

  16. Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR

    Directory of Open Access Journals (Sweden)

    Susan D'Costa

    2016-01-01

    Full Text Available Clinical trials using recombinant adeno-associated virus (rAAV vectors have demonstrated efficacy and a good safety profile. Although the field is advancing quickly, vector analytics and harmonization of dosage units are still a limitation for commercialization. AAV reference standard materials (RSMs can help ensure product safety by controlling the consistency of assays used to characterize rAAV stocks. The most widely utilized unit of vector dosing is based on the encapsidated vector genome. Quantitative polymerase chain reaction (qPCR is now the most common method to titer vector genomes (vg; however, significant inter- and intralaboratory variations have been documented using this technique. Here, RSMs and rAAV stocks were titered on the basis of an inverted terminal repeats (ITRs sequence-specific qPCR and we found an artificial increase in vg titers using a widely utilized approach. The PCR error was introduced by using single-cut linearized plasmid as the standard curve. This bias was eliminated using plasmid standards linearized just outside the ITR region on each end to facilitate the melting of the palindromic ITR sequences during PCR. This new “Free-ITR” qPCR delivers vg titers that are consistent with titers obtained with transgene-specific qPCR and could be used to normalize in-house product-specific AAV vector standards and controls to the rAAV RSMs. The free-ITR method, including well-characterized controls, will help to calibrate doses to compare preclinical and clinical data in the field.

  17. Practical utilization of recombinant AAV vector reference standards: focus on vector genomes titration by free ITR qPCR.

    Science.gov (United States)

    D'Costa, Susan; Blouin, Veronique; Broucque, Frederic; Penaud-Budloo, Magalie; François, Achille; Perez, Irene C; Le Bec, Christine; Moullier, Philippe; Snyder, Richard O; Ayuso, Eduard

    2016-01-01

    Clinical trials using recombinant adeno-associated virus (rAAV) vectors have demonstrated efficacy and a good safety profile. Although the field is advancing quickly, vector analytics and harmonization of dosage units are still a limitation for commercialization. AAV reference standard materials (RSMs) can help ensure product safety by controlling the consistency of assays used to characterize rAAV stocks. The most widely utilized unit of vector dosing is based on the encapsidated vector genome. Quantitative polymerase chain reaction (qPCR) is now the most common method to titer vector genomes (vg); however, significant inter- and intralaboratory variations have been documented using this technique. Here, RSMs and rAAV stocks were titered on the basis of an inverted terminal repeats (ITRs) sequence-specific qPCR and we found an artificial increase in vg titers using a widely utilized approach. The PCR error was introduced by using single-cut linearized plasmid as the standard curve. This bias was eliminated using plasmid standards linearized just outside the ITR region on each end to facilitate the melting of the palindromic ITR sequences during PCR. This new "Free-ITR" qPCR delivers vg titers that are consistent with titers obtained with transgene-specific qPCR and could be used to normalize in-house product-specific AAV vector standards and controls to the rAAV RSMs. The free-ITR method, including well-characterized controls, will help to calibrate doses to compare preclinical and clinical data in the field.

  18. Adeno-associated Virus Serotype 9 - Driven Expression of BAG3 Improves Left Ventricular Function in Murine Hearts with Left Ventricular Dysfunction Secondary to a Myocardial Infarction.

    Science.gov (United States)

    Knezevic, Tijana; Myers, Valerie D; Su, Feifei; Wang, JuFang; Song, Jianliang; Zhang, Xue-Qian; Gao, Erhe; Gao, Guofeng; Muniswamy, Madesh; Gupta, Manish K; Gordon, Jennifer; Weiner, Kristen N; Rabinowitz, Joseph; Ramsey, Frederick V; Tilley, Douglas G; Khalili, Kamel; Cheung, Joseph Y; Feldman, Arthur M

    2016-12-01

    The present study was undertaken to test the hypothesis that gene delivery of BCL2-Associated Athanogene 3 (BAG3) to the heart of mice with left ventricular dysfunction secondary to a myocardial infarction could enhance cardiac performance. BAG3 is a 575 amino acid protein that has pleotropic functions in the cell including pro-autophagy and anti-apoptosis. Mutations in BAG3 have been associated with both skeletal muscle dysfunction and familial dilated cardiomyopathy and BAG3 levels are diminished in non-familial heart failure. Eight-week-old C57/BL6 mice underwent ligation of the left coronary artery (MI) or sham surgery (Sham). Eight weeks later, mice in both groups were randomly assigned to receive either a retro-orbital injection of rAAV9-BAG3 (MI-BAG3 or Sham-BAG3) or rAAV9-GFP (MI-GFP or Sham GFP). Mice were sacrificed at 3 weeks post-injection and myocytes were isolated from the left ventricle. MI-BAG3 mice demonstrated a significantly (p BAG3 injection with further improvement in LVEF, fractional shortening and stroke volume at 3 weeks post-injection without changes in LV mass or LV volume. Injection of rAAV9-BAG3 had no effect on LVEF in Sham mice. The salutary benefits of rAAV9-BAG3 were also observed in myocytes isolated from MI hearts including improved cell shortening (pBAG3 gene therapy may provide a novel therapeutic option for the treatment of heart failure.

  19. Adeno-associated Virus Serotype 9 – Driven Expression of BAG3 Improves Left Ventricular Function in Murine Hearts with Left Ventricular Dysfunction Secondary to a Myocardial Infarction

    Science.gov (United States)

    Knezevic, Tijana; Myers, Valerie D.; Su, Feifei; Wang, JuFang; Song, Jianliang; Zhang, Xue-Qian; Gao, Erhe; Gao, Guofeng; Muniswamy, Madesh; Gupta, Manish K.; Gordon, Jennifer; Weiner, Kristen N.; Rabinowitz, Joseph; Ramsey, Frederick V.; Tilley, Douglas G.; Khalili, Kamel; Cheung, Joseph Y.; Feldman, Arthur M.

    2016-01-01

    Objectives The present study was undertaken to test the hypothesis that gene delivery of BCL2-Associated Athanogene 3 (BAG3) to the heart of mice with left ventricular dysfunction secondary to a myocardial infarction could enhance cardiac performance. Background BAG3 is a 575 amino acid protein that has pleotropic functions in the cell including pro-autophagy and anti-apoptosis. Mutations in BAG3 have been associated with both skeletal muscle dysfunction and familial dilated cardiomyopathy and BAG3 levels are diminished in non-familial heart failure. Methods Eight-week-old C57/BL6 mice underwent ligation of the left coronary artery (MI) or sham surgery (Sham). Eight weeks later, mice in both groups were randomly assigned to receive either a retro-orbital injection of rAAV9-BAG3 (MI-BAG3 or Sham-BAG3) or rAAV9-GFP (MI-GFP or Sham GFP). Mice were sacrificed at 3 weeks post-injection and myocytes were isolated from the left ventricle. Results MI-BAG3 mice demonstrated a significantly (p BAG3 injection with further improvement in LVEF, fractional shortening and stroke volume at 3 weeks post-injection without changes in LV mass or LV volume. Injection of rAAV9-BAG3 had no effect on LVEF in Sham mice. The salutary benefits of rAAV9-BAG3 were also observed in myocytes isolated from MI hearts including improved cell shortening (pBAG3 gene therapy may provide a novel therapeutic option for the treatment of heart failure. PMID:28164169

  20. Restoration of central nervous system alpha-N-acetylglucosaminidase activity and therapeutic benefits in mucopolysaccharidosis IIIB mice by a single intracisternal recombinant adeno-associated viral type 2 vector delivery.

    Science.gov (United States)

    Fu, Haiyan; DiRosario, Julianne; Kang, Lu; Muenzer, Joseph; McCarty, Douglas M

    2010-07-01

    Finding efficient central nervous system (CNS) delivery approaches has been the major challenge facing therapeutic development for treating diseases with global neurological manifestation, such as mucopolysaccharidosis (MPS) IIIB, a lysosomal storage disease, caused by autosomal recessive defect of alpha-N-acetylglucosaminidase (NaGlu). Previously, we developed an approach, intracisternal (i.c.) injection, to deliver recombinant adeno-associated viral (rAAV) vector to the CNS of mice, leading to a widespread periventricular distribution of transduction. In the present study, we delivered rAAV2 vector expressing human NaGlu into the CNS of MPS IIIB mice by an i.c. injection approach, to test its therapeutic efficacy and feasibility for treating the neurological manifestation of the disease. We demonstrated significant functional neurological benefits of a single i.c. vector infusion in adult MPS IIIB mice. The treatment slowed the disease progression by mediating widespread recombinant NaGlu expression in the CNS, resulting in the reduction of brain lysosomal storage pathology, significantly improved cognitive function and prolonged survival. However, persisting motor function deficits suggested that pathology in areas outside the CNS contributes to the MPS IIIB behavioral phenotype. The therapeutic benefit of i.c. rAAV2 delivery was dose-dependent and could be attribute solely to the CNS transduction because the procedure did not lead to detectable transduction in somatic tissues. A single IC rAAV2 gene delivery is functionally beneficial for treating the CNS disease of MPS IIIB in mice. It is immediately clinically translatable, with the potential of improving the quality of life for patients with MPS IIIB.

  1. Recombinant AAV-mediated BEST1 transfer to the retinal pigment epithelium: analysis of serotype-dependent retinal effects.

    Directory of Open Access Journals (Sweden)

    Karina E Guziewicz

    Full Text Available Mutations in the BEST1 gene constitute an underlying cause of juvenile macular dystrophies, a group of retinal disorders commonly referred to as bestrophinopathies and usually diagnosed in early childhood or adolescence. The disease primarily affects macular and paramacular regions of the eye leading to major declines in central vision later in life. Currently, there is no cure or surgical management for BEST1-associated disorders. The recently characterized human disease counterpart, canine multifocal retinopathy (cmr, recapitulates a full spectrum of clinical and molecular features observed in human bestrophinopathies and offers a valuable model system for development and testing of therapeutic strategies. In this study, the specificity, efficiency and safety of rAAV-mediated transgene expression driven by the human VMD2 promoter were assessed in wild-type canine retinae. While the subretinal delivery of rAAV2/1 vector serotype was associated with cone damage in the retina when BEST1 and GFP were co-expressed, the rAAV2/2 vector serotype carrying either GFP reporter or BEST1 transgene under control of human VMD2 promoter was safe, and enabled specific transduction of the RPE cell monolayer that was stable for up to 6 months post injection. These encouraging studies with the rAAV2/2 vector lay the groundwork for development of gene augmentation therapy for human bestrophinopathies.

  2. A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair

    Directory of Open Access Journals (Sweden)

    Leclerc Xavier

    2009-04-01

    Full Text Available Abstract Background Current strategies for gene therapy of inherited diseases consist in adding functional copies of the gene that is defective. An attractive alternative to these approaches would be to correct the endogenous mutated gene in the affected individual. This study presents a quantitative comparison of the repair efficiency using different forms of donor nucleic acids, including synthetic DNA oligonucleotides, double stranded DNA fragments with sizes ranging from 200 to 2200 bp and sequences carried by a recombinant adeno-associated virus (rAAV-1. Evaluation of each gene repair strategy was carried out using two different reporter systems, a mutated eGFP gene or a dual construct with a functional eGFP and an inactive luciferase gene, in several different cell systems. Gene targeting events were scored either following transient co-transfection of reporter plasmids and donor DNAs, or in a system where a reporter construct was stably integrated into the chromosome. Results In both episomal and chromosomal assays, DNA fragments were more efficient at gene repair than oligonucleotides or rAAV-1. Furthermore, the gene targeting frequency could be significantly increased by using DNA repair stimulating drugs such as doxorubicin and phleomycin. Conclusion Our results show that it is possible to obtain repair frequencies of 1% of the transfected cell population under optimized transfection protocols when cells were pretreated with phleomycin using rAAV-1 and dsDNA fragments.

  3. Detection of exogenous gene doping of IGF-I by a real-time quantitative PCR assay.

    Science.gov (United States)

    Zhang, Jin-Ju; Xu, Jing-Feng; Shen, Yong-Wei; Ma, Shi-Jiao; Zhang, Ting-Ting; Meng, Qing-Lin; Lan, Wen-Jun; Zhang, Chun; Liu, Xiao-Mei

    2017-07-01

    Gene doping can be easily concealed since its product is similar to endogenous protein, making its effective detection very challenging. In this study, we selected insulin-like growth factor I (IGF-I) exogenous gene for gene doping detection. First, the synthetic IGF-I gene was subcloned to recombinant adeno-associated virus (rAAV) plasmid to produce recombinant rAAV2/IGF-I-GFP vectors. Second, in an animal model, rAAV2/IGF-I-GFP vectors were injected into the thigh muscle tissue of mice, and then muscle and blood specimens were sampled at different time points for total DNA isolation. Finally, real-time quantitative PCR was employed to detect the exogenous gene doping of IGF-I. In view of the characteristics of endogenous IGF-I gene sequences, a TaqMan probe was designed at the junction of exons 2 and 3 of IGF-I gene to distinguish it from the exogenous IGF-I gene. In addition, an internal reference control plasmid and its probe were used in PCR to rule out false-positive results through comparison of their threshold cycle (Ct) values. Thus, an accurate exogenous IGF-I gene detection approach was developed in this study. © 2016 International Union of Biochemistry and Molecular Biology, Inc.

  4. Protective CD8+ T-cell responses to cytomegalovirus driven by rAAV/GFP/IE1 loading of dendritic cells

    Directory of Open Access Journals (Sweden)

    Dalle-Donne Isabella

    2008-10-01

    Full Text Available Abstract Background Recent studies demonstrate that recombinant adeno-associated virus (rAAV-based antigen loading of dendritic cells (DCs generates in vitro, significant and rapid cytotoxic T-lymphocyte (CTL responses against viral antigens. Methods We used the rAAV system to induce specific CTLs against CVM antigens for the development of cytomegalovirus HCMV gene therapy. As an extension of the versatility of the rAAV system, we incorporated immediate-early 1 (IE1, expressed in HCMV. Our rAAV vector induced a strong stimulation of CTLs directed against the HCMV antigen IE1. We then investigated the efficiency of the CTLs in killing IE1 targeted cells. Results A significant MHC Class I-restricted, anti-IE1-specific CTL killing was demonstrated against IE1 positive peripheral blood mononuclear cells (PBMC after one, in vitro, stimulation. Conclusion In summary, single PBMC stimulation with rAAV/IE1 pulsed DCs induces strong antigen specific-CTL generation. CTLs were capable to lyse low doses of peptides pulsed into target cells. These data suggest that AAV-based antigen loading of DCs is highly effective for generating human CTL responses against HCMV antigens.

  5. Efficient photoreceptor-targeted gene expression in vivo by recombinant adeno-associated virus.

    Science.gov (United States)

    Flannery, J G; Zolotukhin, S; Vaquero, M I; LaVail, M M; Muzyczka, N; Hauswirth, W W

    1997-06-24

    We describe a general approach for achieving efficient and cell type-specific expression of exogenous genes in photoreceptor cells of the mammalian retina. Recombinant adeno-associated virus (rAAV) vectors were used to transfer the bacterial lacZ gene or a synthetic green fluorescent protein gene (gfp) to mouse or rat retinas after injection into the subretinal space. Using a proximal murine rod opsin promoter (+86 to -385) to drive expression, reporter gene product was found exclusively in photoreceptors, not in any other retinal cell type or in the adjacent retinal pigment epithelium. GFP-expressing photoreceptors typically encompassed 10-20% of the total retinal area after a single 2-microl injection. Photoreceptors were transduced with nearly 100% efficiency in the region directly surrounding the injection site. We estimate approximately 2.5 million photoreceptors were transduced as a result of the single subretinal inoculation. This level of gene transfer and expression suggests the feasibility of genetic therapy for retinal disease. The gfp-containing rAAV stock was substantially free of both adenovirus and wild-type AAV, as judged by plaque assay and infectious center assay, respectively. Thus, highly purified, helper virus-free rAAV vectors can achieve high-frequency tissue-specific transduction of terminally differentiated, postmitotic photoreceptor cells.

  6. Areen küsib : mis oli aasta kultuurishokk?

    Index Scriptorium Estoniae

    2005-01-01

    Positiivne ja negatiivne kultuurisündmus 2005. aastal. Küsimusele vastavad Juhan Maiste, Karin Hallas-Murula, Jaan Toomik, Toivo Raidmets, Maile Grünberg, Leo Lapin, Märt Väljataga, Berk Vaher, Sergei Stadnikov, Veljo Kaasik, Margit Mutso, Erkki-Sven Tüür, Mihkel Mutt, Kaarel Tarand, Priidu Beier, Hasso Krull, Jaan Pehk, Hendrik Luuk, Karlo Funk, Kristo Rajasaare, Andres Maimik, Priit Raud, Marianne Kõrver

  7. Dermal Absorption of Nanomaterials Titanium Dioxide and Zinc Oxide Based Sunscreen

    DEFF Research Database (Denmark)

    Beer, Christiane; Dokkedahl, Karin Stenderup; Wang, Jing

    at Aarhus University and has been headed by Ass.Prof. Christiane Beer as project leader and scientific manager with significant contributions by the project collaborates and associated scientists Ass.Prof. Karin Stenderup, Dr. Jing Wang, Prof. Jens Randel Nyengaard, and Ass.Prof. Duncan S. Sutherland......-2015 on Better Control of Nanomaterials and their Safety (“Bedre styr på nano”)....

  8. Acute pancreatitis: a case report

    Directory of Open Access Journals (Sweden)

    Nafia Ozlem Kazanci

    2013-06-01

    Full Text Available Akut pankreatit cocukluk caginda heterojen ve ozgul olmayan klinik bulgularla seyreden, genellikle travma, yapisal anomaliler, ilaclar ve kronik sistemik hastalik zeminde gelisen nadir gorulen bir hastaliktir.Bu olgu sunumunda daha once saglikli oldugu bilinen, karin agrisi yakinmasi ile basvuran ve akut pankreatit tanisi konulan 4 yasinda kiz hastaya yer verildi.Akut pankreatitin idiopatik olarak gelisebildigi ve ozgul olmayan klinik bulgulari nedeniyle tanida yasanabilecek guclukler goz onunde bulundurulmalidir. [J Contemp Med 2013; 3(2.000: 129-132

  9. Kriisimängud Berliinis : "Theatertreffen 2010" / Madli Pesti

    Index Scriptorium Estoniae

    Pesti, Madli, 1980-

    2010-01-01

    7.-24. maini Berliinis toimunud teatrifestivalist "Theatertreffen". Lavastustest "Räpased, koledad, alatud" (lav. Karin Beier), "Riesenbutzbach. Igavene koloonia" (lav. Marthaler), Dennis Kelly "Ma olen nii armunud, et võiksin oksendada" (lav. Stephan Kimmig), Roland Schimmelpfennigiti "Kuldne draakon" (lav. R. Schimmelpfennig), Peter Handke "Tund, mil me üksteist midagi ei teadnud" (lav. Viktor Bodo) ja Elfride Jelineki tekstide põhjal "Kaupmehe kontraht" (lav. Nicolas Stemann)

  10. Noorte eesti režissööride uued filmid Sõpruses / Mari Peegel

    Index Scriptorium Estoniae

    Peegel, Mari, 1978-

    2005-01-01

    Sõpruse kinos esilinastub homme kaks eesti lühimängufilmi - "Vanameeste paradiis" : stsenaristid Ove Musting, Urmas Lennuk : režissöör Ove Musting : mängivad Tõnu Aav, Aleksander Eelmaa jt ning "Ukse taga" : stsenarist Margit Keerdo : režissöör Andrus Tuisk : osades Hele Kõre, Karin Lätsim, Kirill Käro, Igor Gavrilov

  11. Esilinastuseni jõuab kaks uut Eesti lühimängufilmi

    Index Scriptorium Estoniae

    2005-01-01

    Sõpruse kinos esilinastub homme kaks eesti lühimängufilmi - "Vanameeste paradiis" : stsenaristid Ove Musting, Urmas Lennuk : režissöör Ove Musting : mängivad Tõnu Aav, Aleksander Eelmaa jt ning "Ukse taga" : stsenarist Margit Keerdo : režissöör Andrus Tuisk : osades Hele Kõre, Karin Lätsim, Kirill Käro, Igor Gavrilov

  12. Review of National Work Programme on the Long Term Effects of Sustained High G on the Cervical Spine (Analyse du programme de travail national : les effets long terme sur la colonne cervicale d’un nombre de G lev et prolong )

    Science.gov (United States)

    2008-12-01

    Neck Muscle 6-7 Function and Symptoms of Neck Pain in UK Student Pilots 6.3.3 Other Ongoing Relevant Activities 6-9 6.4 Unclassified Reports...Prof. Karin Harms-Ringdahl, PhD, RPT Karolinska Institutet Department of Neurobiology, Care Sciences, and Society Division of Physiotherapy 23100...Äng Karolinska Institutet Department of Neurobiology, Care Sciences, and Society Division of Physiotherapy Alfred Nobels Allé 23100 SE-14183

  13. Hästi restaureeritud raekoda sai Euroopast kõrge tunnustuse / Askur Alas

    Index Scriptorium Estoniae

    Alas, Askur, 1973-

    2006-01-01

    Tallinna raekoda sai Euroopa Liidu ja Euroopa pärandkultuuri organisatsiooni Europa Nostra kultuuripärandi medali. Europa Nostra nõukogu liikme Karin Hallas-Murula sõnul arvestatakse auhindade jagamisel nii mälestise säilitamist kui ka kasutamist tänapäeval. Mainekas auhind on tunnustus Teddy Böcklerile, kellele raekoja restaureerimine oli elutööks. Lisatud Europa Nostra peapreemiad

  14. Perspectives on Positioning, Interaction, and Learning in Small-Group Discussion: Possibilities for Extending the Analytic Lens

    Science.gov (United States)

    Kittleson, Julie M.; Wilson, Rachel E.

    2014-01-01

    In this forum piece, we respond to Karin Due's study of social dynamics in groups of students in physics class and gender issues that play out in this context. We discuss two threads that appear in Due's paper: one pertains to patterns of talk within groups and how these patterns open up possibilities for learning, the other pertains to…

  15. Long Term Cost Efficiency through Green Management Control Systems

    OpenAIRE

    Vukania Adda, Nancy; Qin, Xiaochen

    2012-01-01

    Title: Long term cost efficiency through green management control systems.Authors: Nancy Vukania &Xiaochen QinSupervisor: Åsa Karin-EngstrandBackground: The worldwide financial crisis of 2008 has reconfigured the economic turf leading to a more uncertain and turbulent playing field – a greater challenge for business strategy and the quest for optimization- The oil price hike of 2008 (Furlong 2010)1 caused its rippling effect to affect various cost categories including energy, labor and lo...

  16. Karl Menningu (ja iseenese) päästmiseks / Ants Hein

    Index Scriptorium Estoniae

    Hein, Ants, 1952-

    2008-01-01

    Vastuseks Karin Hallas-Murula arvustusele "Maja, mis on sümbol" (Sirp, 2007, 5. X, lk. 13) ja Mait Väljase arvustusele "Iseolemise algus arhitektuuris" (Maja, 2008, nr. 1, lk. 86-87) oma 2007. a. ilmunud raamatust "Maja kui sümbol : Eesti Üliõpilaste Seltsi hoone Tartus" kohta. Georg Hellati ja Karl Menningu osalusest EÜS-i hoone sünniloos. Frank Lloyd Wrightist kui võimalikust eeskujuandjast

  17. Ööpäeva väldanud etendused keerasid näitlejate ja publiku elurütmi pea peale / Marika Rajamäe, Merili Nikkolo

    Index Scriptorium Estoniae

    Rajamäe, Marika, 1952-

    2008-01-01

    Vargamäel 22 tundi kestnud maratonetendusest Tammsaare "Tõe ja õiguse" põhjal - Elmo Nüganeni lavastused "Tõde ja õigus. Teine osa", "Karin. Indrek. Tõde ja õigus. 4" ja "Wargamäe Wabariik" (III osa) ning Jaanus Rohumaa lavastus "Vargamäe kuningriik" (I ja V osa). Reportaaž on tähtsamatest hetkedest alates 21. juuni kell 11.00 kuni 22. juuni 10.00-ni

  18. Proceedings of the 3rd Biennial Conference of the Society for Implementation Research Collaboration (SIRC) 2015: advancing efficient methodologies through community partnerships and team science

    OpenAIRE

    Lewis, Cara; Darnell, Doyanne; Kerns, Suzanne; Monroe-DeVita, Maria; Landes, Sara J.; Lyon, Aaron R.; Stanick, Cameo; Dorsey, Shannon; Locke, Jill; Marriott, Brigid; Puspitasari, Ajeng; Dorsey, Caitlin; Hendricks, Karin; Pierson, Andria; Fizur, Phil

    2016-01-01

    Table of contents Introduction to the 3rd Biennial Conference of the Society for Implementation Research Collaboration: advancing efficient methodologies through team science and community partnerships Cara Lewis, Doyanne Darnell, Suzanne Kerns, Maria Monroe-DeVita, Sara J. Landes, Aaron R. Lyon, Cameo Stanick, Shannon Dorsey, Jill Locke, Brigid Marriott, Ajeng Puspitasari, Caitlin Dorsey, Karin Hendricks, Andria Pierson, Phil Fizur, Katherine A. Comtois A1: A behavioral economic perspective ...

  19. Trichtlinnburg tõi esile asjad, mida turist ei näe / Liina Siib

    Index Scriptorium Estoniae

    Siib, Liina

    2005-01-01

    Ajaloolise linnaruumi säilitamist ja arendamist uuriva Euroopa kultuuriprojekti Trichtlinnburg (TLB) Tallinnas toimunud kunstiprojektidest. Sanja Ivekovici video "(If) I Lived Here", Silja Saarepuu ja Villu Plingi objekt "Plats", Anna-Karin Stjerlofi näidispostkaardid, Jüri Ojaveri projekt "Pääsla", Tiina Tammetalu projekt "Seirajad", Katrin Essensoni ja Helen Becki performance'id, David Bate'i fotosari, TLB jalutuskäigud Tallinnas

  20. Identifying and Leveraging Trust as a Key Element in the Development, Implementation and Sustainment of the Salt Lake City Fire Department’s Intelligence Program

    Science.gov (United States)

    2014-09-01

    this amazing adventure. An education in self-exploration balanced with relentless tests of my stamina and mettle. To my classmates: Thank you for...Karin and son Scott, thank you for providing the comic relief so needed throughout this process. And most importantly, to my husband, Jeff: There...development of a fire intelligence program.190191 In her NPS Masters Thesis, Terrorism Awareness and Education as a Prevention Strategy for First

  1. Eile sai Eesti Kunstimuuseumi asutamisest 86 aastat ning Tartu Kunstimuuseumi asutamisest 65 aastat

    Index Scriptorium Estoniae

    2005-01-01

    Kumu ettekannetepäev "Roheline, toores ja lõikamata. Noor eesti kunst uue sajandi alguses" sarjast "Lisandusi eesti kunstiloole". Esinesid Karin Laansoo, Irit Rogoff, Kaido Ole, Kristjan Mändmaa, Rael Artel. Esitleti raamatut "22 + Noored eesti kunstnikud. Young Estonian Artists". 18. XI kirjutatakse alla Jaapani valitsuse kultuuritoetusleping, mis on suunatud Kumu varustamiseks kaasaegse audiogiidi süsteemiga, esitlustehnika ja maalikonserveerimislabori tehnikaga

  2. Browse Author Index

    African Journals Online (AJOL)

    A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All. W. Walker, Melanie · Walters, Shirley · Williams, Kevin · Winberg, Chris · Winberg, Christine · Wisker, Gina · Wolff, Karin 1 - 7 of 7 Items. ISSN: 2310-7103. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More ...

  3. Equitable PERSTEMPO - The Challenge for Long-Term Deployed Armed Forces

    Science.gov (United States)

    2003-04-07

    substantial impact on the operational effectiveness of the Bundeswehr of the future.  Rudolf Scharping Former Federal Minister of Defense The vision...Federal Minister of Defense, Rudolf Scharping, initiated a new force planning by means of the so-called "Cornerstones Paper" on 01 June 2000, the Chief of...Annex 1) 77 Rainer Marr, Timea Biro, and Karin Steiner , Vereinbarkeit von Familie und Beruf des Soldaten – Moeglichkeiten und Grenzen einer

  4. By Process of Elimination: Further Remarks on the Operation chú 除 in Early Imperial Mathematical Astronomy

    OpenAIRE

    Morgan, Daniel Patrick

    2015-01-01

    International audience; This paper will return to the operation chu 除 (“eliminate”) as explored by Karine Chemla and myself on January 23, refining the terminology deployed, providing usage statistics, and exploring actors’ rare words of definition and expository. We will revisit how chu “eliminate” is used to prescribe (1) subtraction for a positive integer difference, (2) repeated subtraction of a single value for a positive integer remainder, i.e. modulo, (3) subtraction of a series of val...

  5. Historiens Mening som et Kreativt Produkt - En Analyse av Camille Paglias "The Birth of the Western Eye"

    OpenAIRE

    Kyllingstad, Elisabeth

    2016-01-01

    Dette prosjektet er en metodisk tilnærming til Camille Paglias filosofiske historietekst ”The Birth of the Western Eye”. Sexual Personae – Art and Decadence from Nefertiti to Emily Dickinson (1991) er en revidert versjon av doktorgradsavhendlingen hennes, som hun forsvarte i 1974. Den forteller om det Paglia identifiserer som grunnleggende drivkrefter i det vestlige mennesket, og dets kunstneriske produksjon. På denne måten kan verket leses som en guide gjennom vestlig tenkning så vel som kun...

  6. Assessing the Influence of Fashion Clothing Advertising on Women's Consumer Behaviour in Finland; a case study of H&M

    OpenAIRE

    Hamed Abu Adab, Sari

    2012-01-01

    This study will provide a framework for analysing the current advertising and marketing patterns in women’s consumer behaviour in Finland. Swedish clothing retailer Hennes&Mauritz (H&M) was chosen as a case study since it is considered to be well-known in Finland; in 2010 average sales were astonishingly around 243million euros (H&M 2012). As this research is considered to be a broad topic, this study will focus on women in Finland aged 16-35 and above. This study will use various research m...

  7. The CSR Challenges in the Clothing Industry

    Directory of Open Access Journals (Sweden)

    Paulina Księżak

    2016-10-01

    Full Text Available The aim of this paper is to study the clothing and textile industry through the prism of the CSR. First of all, it investigates the ways in which companies of this sector deal with the social responsibility issues that are specifically important to the characteristics of the fashion industry. Secondly, the motivators of implementing CSR in the textile and clothing business are identified and analysed. Thirdly, the CSR practices of H&M Hennes & Mauritz AB, a wellknown multinational corporation operating in the sector of fashion, are discussed in order to illustrate the theoretical review with the case study approach.

  8. The importance of strategic management : A case study of H&M

    OpenAIRE

    Ding, Huiru

    2011-01-01

    Hennes & Mauritz (H&M) is a 100 billion Sweden company,engaged in designing and retailing of fashion apparel and accessories. The company offers a range of apparel, cosmetics, footwear and accessories for men, women, children and teenagers. H&M primarily operates in Europe, North America and Asia, and has a presence in over 38 countries. The company is headquartered in Stockholm, Sweden and employs approximately 87,000 people on a full-time basis. This thesis focused on the strategic...

  9. epsilon : A tool to find a canonical basis of master integrals

    Science.gov (United States)

    Prausa, Mario

    2017-10-01

    In 2013, Henn proposed a special basis for a certain class of master integrals, which are expressible in terms of iterated integrals. In this basis, the master integrals obey a differential equation, where the right hand side is proportional to ɛ in d = 4 - 2 ɛ space-time dimensions. An algorithmic approach to find such a basis was found by Lee. We present the tool epsilon, an efficient implementation of Lee's algorithm based on the Fermat computer algebra system as computational back end.

  10. Long-term gene therapy causes transgene-specific changes in the morphology of regenerating retinal ganglion cells.

    Directory of Open Access Journals (Sweden)

    Jennifer Rodger

    Full Text Available Recombinant adeno-associated viral (rAAV vectors can be used to introduce neurotrophic genes into injured CNS neurons, promoting survival and axonal regeneration. Gene therapy holds much promise for the treatment of neurotrauma and neurodegenerative diseases; however, neurotrophic factors are known to alter dendritic architecture, and thus we set out to determine whether such transgenes also change the morphology of transduced neurons. We compared changes in dendritic morphology of regenerating adult rat retinal ganglion cells (RGCs after long-term transduction with rAAV2 encoding: (i green fluorescent protein (GFP, or (ii bi-cistronic vectors encoding GFP and ciliary neurotrophic factor (CNTF, brain-derived neurotrophic factor (BDNF or growth-associated protein-43 (GAP43. To enhance regeneration, rats received an autologous peripheral nerve graft onto the cut optic nerve of each rAAV2 injected eye. After 5-8 months, RGCs with regenerated axons were retrogradely labeled with fluorogold (FG. Live retinal wholemounts were prepared and GFP positive (transduced or GFP negative (non-transduced RGCs injected iontophoretically with 2% lucifer yellow. Dendritic morphology was analyzed using Neurolucida software. Significant changes in dendritic architecture were found, in both transduced and non-transduced populations. Multivariate analysis revealed that transgenic BDNF increased dendritic field area whereas GAP43 increased dendritic complexity. CNTF decreased complexity but only in a subset of RGCs. Sholl analysis showed changes in dendritic branching in rAAV2-BDNF-GFP and rAAV2-CNTF-GFP groups and the proportion of FG positive RGCs with aberrant morphology tripled in these groups compared to controls. RGCs in all transgene groups displayed abnormal stratification. Thus in addition to promoting cell survival and axonal regeneration, vector-mediated expression of neurotrophic factors has measurable, gene-specific effects on the morphology of injured

  11. A translational approach for limb vascular delivery of the micro-dystrophin gene without high volume or high pressure for treatment of Duchenne muscular dystrophy

    Directory of Open Access Journals (Sweden)

    Chicoine Louis G

    2007-09-01

    Full Text Available Abstract Background Duchenne muscular dystrophy (DMD is an X-linked recessive disorder with monogenic mutations setting the stage for successful gene therapy treatment. We have completed a study that directly deals with the following key issues that can be directly adapted to a gene therapy clinical trial using rAAV considering the following criteria: 1 A regional vascular delivery approach that will protect the patient from widespread dissemination of virus; 2 an approach to potentially facilitate safe passage of the virus for efficient skeletal muscle transduction; 3 the use of viral doses to accommodate current limitations imposed by vector production methods; 4 and at the same time, achieve a clinically meaningful outcome by transducing multiple muscles in the lower limb to prolong ambulation. Methods The capacity of AAV1, AAV6 or AAV8 to cross the vascular endothelial barrier carrying a micro-dystrophin cDNA was compared under identical conditions with delivery through a catheter placed in the femoral artery of the mdx mouse. Transduction efficiency was assessed by immuno-staining using an antibody (Manex1a that recognizes the N-terminus of micro-dystrophin. The degree of physiologic correction was assessed by measuring tetanic force and protection from eccentric contraction in the extensor digitorum longus muscle (EDL. The vascular delivery paradigm found successful in the mouse was carried to the non-human primate to test its potential translation to boys with DMD. Results Regional vascular delivery resulted in transduction by rAAV8.micro-dystrophin reaching 94.5 ± 0.9 (1 month, 91.3 ± 3.1 (2 months, and 89.6 ± 1.6% (3 months. rAAV6.micro-dystrophin treated animals demonstrated 87.7 ± 6.8 (1 month, 78.9 ± 7.4 (2 months, and 81.2 ± 6.2% (3 months transduction. In striking contrast, rAAV1 demonstrated very low transduction efficiency [0.9 ± 0.3 (1 month, 2.1 ± 0.8 (2 months, and 2.1 ± 0.7% (3 months] by vascular delivery. Micro

  12. Algebraic topology VIASM 2012–2015

    CERN Document Server

    Schwartz, Lionel

    2017-01-01

    Held during algebraic topology special sessions at the Vietnam Institute for Advanced Studies in Mathematics (VIASM, Hanoi), this set of notes consists of expanded versions of three courses given by G. Ginot, H.-W. Henn and G. Powell. They are all introductory texts and can be used by PhD students and experts in the field.   Among the three contributions, two concern stable homotopy of spheres: Henn focusses on the chromatic point of view, the Morava K(n)-localization and the cohomology of the Morava stabilizer groups. Powell’s chapter is concerned with the derived functors of the destabilization and iterated loop functors and provides a small complex to compute them. Indications are given for the odd prime case.   Providing an introduction to some aspects of string and brane topology, Ginot’s contribution focusses on Hochschild homology and its generalizations. It contains a number of new results and fills a gap in the literature. .

  13. Three-body correlations in the decay of {sup 10}He and {sup 13}Li

    Energy Technology Data Exchange (ETDEWEB)

    Johansson, H.T. [Fundamental Fysik, Chalmers Tekniska Hoegskola, S-412 96 Goeteborg (Sweden); Aksyutina, Yu. [Fundamental Fysik, Chalmers Tekniska Hoegskola, S-412 96 Goeteborg (Sweden); ExtreMe Matter Institute, EMMI, GSI, D-64291 Darmstadt (Germany); Aumann, T.; Boretzky, K. [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Borge, M.J.G. [Instituto Estructura de la Materia, CSIC, E-28006 Madrid (Spain); Chatillon, A. [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Chulkov, L.V. [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Kurchatov Institute, RU-123182 Moscow (Russian Federation); Cortina-Gil, D. [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); University of Santiago de Compostela, 15706 Santiago de Compostela (Spain); Datta Pramanik, U. [Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064 (India); Emling, H. [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Forssen, C. [Fundamental Fysik, Chalmers Tekniska Hoegskola, S-412 96 Goeteborg (Sweden); Fynbo, H.O.U. [Department of Physics and Astronomy, University of Aarhus, DK-8000 Aarhus C (Denmark); Geissel, H.; Ickert, G. [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Jonson, B., E-mail: Bjorn.Jonson@chalmers.s [Fundamental Fysik, Chalmers Tekniska Hoegskola, S-412 96 Goeteborg (Sweden); Physics Department, CERN, CH-1211 Geneve 23 (Switzerland); Kulessa, R. [Instytut Fizyki, Universytet Jagiellonski, PL-30-059 Krakow (Poland); Langer, C. [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, D-64291 Darmstadt (Germany); Lantz, M. [Fundamental Fysik, Chalmers Tekniska Hoegskola, S-412 96 Goeteborg (Sweden); LeBleis, T.; Mahata, K. [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, D-64291 Darmstadt (Germany)

    2010-12-01

    The very exotic nuclear resonance systems, {sup 10}He and {sup 13}Li, are produced in proton-knockout reactions from relativistic beams of {sup 11}Li and {sup 14}Be. The experimentally determined energy and angular correlations between their decay products, {sup 8}He+n+n and {sup 11}Li+n+n, are analyzed using an expansion of decay amplitudes in a restricted set of hyperspherical harmonics. By considering only a small number of terms it is possible to extract the expansion coefficients directly from the experimental three-body correlations. This provides a model-independent way of getting information about the decay process, on the structure of the decaying nucleus and on the quantum characteristics of the binary subsystems. The results show that the {sup 8}He+n+n relative-energy spectrum can be interpreted as consisting of two resonances, an I{sup {pi}=}0{sup +} ground state and an excited I{sup {pi}=}2{sup +} state. The {sup 11}Li+n+n relative-energy spectrum is interpreted as an I{sup {pi}=}3/2{sup -} ground state overlapping with excited states having a structure similar to the 2{sup +} state in {sup 10}He but spread over several states due to the coupling to the I{sup {pi}=}3/2{sup -} core. The {sup 13}Li data also give evidence for a contribution of a configuration where the two neutrons occupy the d-shell.

  14. Einige Überlegungen zur Bestimmung des Äquivalenzgrades am Beispiel des lexikalischen Feldes „Erhebung im Gelände“

    Directory of Open Access Journals (Sweden)

    Daumantas Katinas

    2013-12-01

    Full Text Available Eine der wichtigsten Fragen in der kontrastiven Lexikologie, zweisprachigen Lexikografie und der Übersetzungswissenschaft ist die Bestimmung des Äquivalenzgrades zwischen den Lexemen der Vergleichssprachen. Die meisten Lexikologen, die sich mit kontrastiven Untersuchungen beschäftigen, sprechen von der Voll-, Teil- und Nulläquivalenz. In der Praxis existiert allerdings nur die so genannte Teiläquivalenz. Wenn man aber in der wissenschaftlichen Diskussion nur diesen Begriff verwenden würde, entstünden somit noch größere Schwierigkeiten bei der Bestimmung des Äquivalenzgrades, weil sich das Spektrum der Teiläquivalenz von 1 bis 99 Prozent erstrecken würde. Daher wird im vorliegenden Beitrag eine Formel zur Berechnung des Äquivalenzgrades zwischen zwei Vergleichslexemen vorgeschlagen. In erster Linie wird die semantische Analyse des lexikalischen Feldes „Erhebung im Gelände“ im Litauischen und im Deutschen, das die Grundlage der Formel bildet, kurz präsentiert. Dazu wird der von Helmut Henne und Herbert Ernst Wiegand (Henne 1972, Wiegand 1970, Kühn 1979 vorgeschlagene Entwurf, die lexikalische Bedeutung mit Hilfe von onomasiologischen, komplementär-semasiologischen und autonom-semasiologischen Operationsschritten zu beschreiben, herangezogen. Dieser Entwurf wird durch kontrastive, prototypensemantische und korpusgestützte Aspekte ergänzt. Im zweiten Teil wird die mathematische Formel zur Berechnung des Äquivalenzgrades zwischen zwei Vergleichslexemen angegeben und durch das Beispiel des Lexempaars kalva–Hügel veranschaulicht.

  15. Chronic Cardiac-Targeted RNA Interference for the Treatment of Heart Failure Restores Cardiac Function and Reduces Pathological Hypertrophy

    Science.gov (United States)

    Suckau, Lennart; Fechner, Henry; Chemaly, Elie; Krohn, Stefanie; Hadri, Lahouaria; Kockskämper, Jens; Westermann, Dirk; Bisping, Egbert; Ly, Hung; Wang, Xiaomin; Kawase, Yoshiaki; Chen, Jiqiu; Liang, Lifan; Sipo, Isaac; Vetter, Roland; Weger, Stefan; Kurreck, Jens; Erdmann, Volker; Tschope, Carsten; Pieske, Burkert; Lebeche, Djamel; Schultheiss, Heinz-Peter; Hajjar, Roger J.; Poller, Wolfgang Ch.

    2009-01-01

    Background RNA interference (RNAi) has the potential to be a novel therapeutic strategy in diverse areas of medicine. We report on targeted RNAi for the treatment of heart failure (HF), an important disorder in humans resulting from multiple etiologies. Successful treatment of HF is demonstrated in a rat model of transaortic banding by RNAi targeting of phospholamban (PLB), a key regulator of cardiac Ca2+ homeostasis. Whereas gene therapy rests on recombinant protein expression as its basic principle, RNAi therapy employs regulatory RNAs to achieve its effect. Methods and Results We describe structural requirements to obtain high RNAi activity from adenoviral (AdV) and adeno-associated virus (AAV9) vectors and show that an AdV short hairpin RNA vector (AdV-shRNA) silenced PLB in cardiomyocytes (NRCMs) and improved hemodynamics in HF rats 1 month after aortic root injection. For simplified long-term therapy we developed a dimeric cardiotropic AAV vector (rAAV9-shPLB) delivering RNAi activity to the heart via intravenous injection. Cardiac PLB protein was reduced to 25% and SERCA2a suppression in the HF groups was rescued. In contrast to traditional vectors rAAV9 shows high affinity for myocardium, but low affinity for liver and other organs. rAAV9-shPLB therapy restored diastolic (LVEDP, dp/dtmin, Tau) and systolic (fractional shortening) functional parameters to normal range. The massive cardiac dilation was normalized and the cardiac hypertrophy, cardiomyocyte diameter and cardiac fibrosis significantly reduced. Importantly, there was no evidence of microRNA deregulation or hepatotoxicity during these RNAi therapies. Conclusion Our data show, for the first time, high efficacy of an RNAi therapeutic strategy in a cardiac disease. PMID:19237664

  16. Catalytic immunoglobulin gene delivery in a mouse model of Alzheimer's disease: prophylactic and therapeutic applications.

    Science.gov (United States)

    Kou, Jinghong; Yang, Junling; Lim, Jeong-Eun; Pattanayak, Abhinandan; Song, Min; Planque, Stephanie; Paul, Sudhir; Fukuchi, Ken-Ichiro

    2015-02-01

    Accumulation of amyloid beta-peptide (Aβ) in the brain is hypothesized to be a causal event leading to dementia in Alzheimer's disease (AD). Aβ vaccination removes Aβ deposits from the brain. Aβ immunotherapy, however, may cause T cell- and/or Fc-receptor-mediated brain inflammation and relocate parenchymal Aβ deposits to blood vessels leading to cerebral hemorrhages. Because catalytic antibodies do not form stable immune complexes and Aβ fragments produced by catalytic antibodies are less likely to form aggregates, Aβ-specific catalytic antibodies may have safer therapeutic profiles than reversibly-binding anti-Aβ antibodies. Additionally, catalytic antibodies may remove Aβ more efficiently than binding antibodies because a single catalytic antibody can hydrolyze thousands of Aβ molecules. We previously isolated Aβ-specific catalytic antibody, IgVL5D3, with strong Aβ-hydrolyzing activity. Here, we evaluated the prophylactic and therapeutic efficacy of brain-targeted IgVL5D3 gene delivery via recombinant adeno-associated virus serotype 9 (rAAV9) in an AD mouse model. One single injection of rAAV9-IgVL5D3 into the right ventricle of AD model mice yielded widespread, high expression of IgVL5D3 in the unilateral hemisphere. IgVL5D3 expression was readily detectable in the contralateral hemisphere but to a much lesser extent. IgVL5D3 expression was also confirmed in the cerebrospinal fluid. Prophylactic and therapeutic injection of rAAV9-IgVL5D3 reduced Aβ load in the ipsilateral hippocampus of AD model mice. No evidence of hemorrhages, increased vascular amyloid deposits, increased proinflammatory cytokines, or infiltrating T-cells in the brains was found in the experimental animals. AAV9-mediated anti-Aβ catalytic antibody brain delivery can be prophylactic and therapeutic options for AD.

  17. Recombinant AAV-mediated HSVtk gene transfer with direct intratumoral injections and Tet-On regulation for implanted human breast cancer

    International Nuclear Information System (INIS)

    Zi-Bo, LI; Zhao-Jun, ZENG; Qian, CHEN; Sai-Qun, LUO; Wei-Xin, HU

    2006-01-01

    HSVtk/ganciclovir (GCV) gene therapy has been extensively studied in tumors and relies largely on the gene expression of HSVtk. Most studies, however, have failed to demonstrate any significant benefit of a controlled gene expression strategy in cancer treatment. The Tet-On system is commonly used to regulate gene expression following Dox induction. We have evaluated the antitumor effect of HSVtk/ganciclovir gene therapy under Tet-On regulation by means of adeno-associated virus-2 (AAV-2)-mediated HSVtk gene transfer with direct intratumoral injections in mice bearing breast cancer tumors. Recombinant adeno-associated virus-2 (rAAV) was constructed and transduced into MCF-7 cell line. GCV treatment to the rAAV infected MCF-7 cells was performed by MTT assay under the doxycycline (Dox) induction or without Dox induction at a vp (viral particle) number of ≥10 4 /cell. The virus was administered intratumorally to nude mice that had also received GCV intraperitoneally. The antitumor effects were evaluated by measuring tumor regression and histological analysis. We have demonstrated that GCV treatment to the infected MCF-7 cells under the Dox induction was of more inhibited effects than those without Dox induction at ≥10 4 vp/cell. In ex vivo experiments, tumor growth of BALB/C nude mice breast cancer was retarded after rAAV-2/HSVtk/Tet-On was injected into the tumors under the Dox induction. Infiltrating cells were also observed in tumors after Dox induction followed by GCV treatment and cells were profoundly damaged. The expression of HSVtk gene in MCF-7 cells and BALB/C nude mice tumors was up-regulated by Tet-On under Dox induction with reverse transcription-PCR (RT-PCR) analysis. The antitumor effect of rAAV-mediated HSVtk/GCV gene therapy under the Dox induction with direct intratumoral injections may be a useful treatment for breast cancer and other solid tumors

  18. Overexpression of the steroidogenic enzyme cytochrome P450 side chain cleavage in the ventral tegmental area increases 3α,5α-THP and reduces long-term operant ethanol self-administration.

    Science.gov (United States)

    Cook, Jason B; Werner, David F; Maldonado-Devincci, Antoniette M; Leonard, Maggie N; Fisher, Kristen R; O'Buckley, Todd K; Porcu, Patrizia; McCown, Thomas J; Besheer, Joyce; Hodge, Clyde W; Morrow, A Leslie

    2014-04-23

    Neuroactive steroids are endogenous neuromodulators capable of altering neuronal activity and behavior. In rodents, systemic administration of endogenous or synthetic neuroactive steroids reduces ethanol self-administration. We hypothesized this effect arises from actions within mesolimbic brain regions that we targeted by viral gene delivery. Cytochrome P450 side chain cleavage (P450scc) converts cholesterol to pregnenolone, the rate-limiting enzymatic reaction in neurosteroidogenesis. Therefore, we constructed a recombinant adeno-associated serotype 2 viral vector (rAAV2), which drives P450scc expression and neuroactive steroid synthesis. The P450scc-expressing vector (rAAV2-P450scc) or control GFP-expressing vector (rAAV2-GFP) were injected bilaterally into the ventral tegmental area (VTA) or nucleus accumbens (NAc) of alcohol preferring (P) rats trained to self-administer ethanol. P450scc overexpression in the VTA significantly reduced ethanol self-administration by 20% over the 3 week test period. P450scc overexpression in the NAc, however, did not alter ethanol self-administration. Locomotor activity was unaltered by vector administration to either region. P450scc overexpression produced a 36% increase in (3α,5α)-3-hydroxypregnan-20-one (3α,5α-THP, allopregnanolone)-positive cells in the VTA, but did not increase 3α,5α-THP immunoreactivity in NAc. These results suggest that P450scc overexpression and the resultant increase of 3α,5α-THP-positive cells in the VTA reduces ethanol reinforcement. 3α,5α-THP is localized to neurons in the VTA, including tyrosine hydroxylase neurons, but not astrocytes. Overall, the results demonstrate that using gene delivery to modulate neuroactive steroids shows promise for examining the neuronal mechanisms of moderate ethanol drinking, which could be extended to other behavioral paradigms and neuropsychiatric pathology.

  19. Positron Emission Tomography Imaging Demonstrates Correlation between Behavioral Recovery and Correction of Dopamine Neurotransmission after Gene Therapy

    International Nuclear Information System (INIS)

    Leriche, L.; Besret, L.; Gregoire, M.C.; Deglon, N.; Hantraye, Ph.; Leriche, L.; Besret, L.; Gregoire, M.C.; Deglon, N.; Hantraye, Ph.; Bjorklund, T.; Breysse, N.; Carlsson, T.; Kirik, D.; Dolle, F.; Mandel, R.J.; Kirik, D.

    2009-01-01

    In vivo gene transfer using viral vectors is an emerging therapy for neuro-degenerative diseases with a clinical impact recently demonstrated in Parkinson's disease patients. Recombinant adeno-associated viral (rAAV) vectors, in particular, provide an excellent tool for long-term expression of therapeutic genes in the brain. Here we used the [ 11 C]raclopride [(S)-(-)-3, 5-dichloro-N-((1-ethyl-2-pyrrolidinyl)methyl)-2-hydroxy- 6-methoxybenzamide] micro-positron emission tomography (PET) technique to demonstrate that delivery of the tyrosine hydroxylase (TH) and GTP-cyclohydrolase 1 (GCH1) enzymes using an rAAV5 vector normalizes the increased [ 11 C]raclopride binding in hemi-parkinsonian rats. Importantly, we show in vivo by micro-PET imaging and postmortem by classical binding assays performed in the very same animals that the changes in [ 11 C]raclopride after viral vector-based enzyme replacement therapy is attributable to a decrease in the affinity of the tracer binding to the D2 receptors, providing evidence for reconstitution of a functional pool of endogenous dopamine in the striatum. Moreover, the extent of the normalization in this non-invasive imaging measure was highly correlated with the functional recovery in motor behavior. The PET imaging protocol used in this study is fully adaptable to humans and thus can serve as an in vivo imaging technique to follow TH+GCH1 gene therapy in PD patients and provide an additional objective measure to a potential clinical trial using rAAV vectors to deliver L-3, 4-dihydroxyphenylalanine in the brain. (authors)

  20. Normal-tissue radioprotection by overexpression of the copper-zinc and manganese superoxide dismutase genes

    Energy Technology Data Exchange (ETDEWEB)

    Veldwijk, Marlon R. [Dept. of Radiation Oncology, Univ. Medical Center Mannheim, Univ. of Heidelberg, Mannheim (Germany); Pharmacology of Cancer Treatment (G402), German Cancer Research Center, Heidelberg (Germany); Herskind, Carsten; Wenz, Frederik [Dept. of Radiation Oncology, Univ. Medical Center Mannheim, Univ. of Heidelberg, Mannheim (Germany); Sellner, Leopold; Zeller, W. Jens [Pharmacology of Cancer Treatment (G402), German Cancer Research Center, Heidelberg (Germany); Radujkovic, Aleksandar [Dept. of Internal Medicine V, Univ. of Heidelberg (Germany); Laufs, Stephanie [Dept. of Experimental Surgery, Univ. Medical Center Mannheim, Univ. of Heidelberg, Mannheim (Germany); Molecular Oncology of Solid Tumors (G360), German Cancer Research Center, Heidelberg (Germany); Fruehauf, Stefan [Center for Tumor Diagnostic and Therapy, Paracelsus-Klinik, Osnabrueck (Germany)

    2009-08-15

    Background and Purpose: Protection of normal tissue against radiation-induced damage may increase the therapeutic ratio of radiotherapy. A promising strategy for testing this approach is gene therapy-mediated overexpression of the copper-zinc (CuZnSOD) or manganese superoxide dismutase (MnSOD) using recombinant adeno-associated viral (rAAV2) vectors. The purpose of this study was to test the modulating effects of the SOD genes on human primary lung fibroblasts (HPLF) after irradiation. Material and Methods: HPLF were transduced with rAAV2 vectors containing cDNA for the CuZnSOD, MnSOD or a control gene. The cells were irradiated (1-6 Gy), and gene transfer efficiency, apoptosis, protein expression/activity, and radiosensitivity measured by the colony-forming assay determined. Results: After transduction, 90.0% {+-} 6.4% of the cells expressed the transgene. A significant fivefold overexpression of both SOD was confirmed by an SOD activity assay (control: 21.1 {+-} 12.6, CuZnSOD: 95.1 {+-} 17.1, MnSOD: 108.5 {+-} 36.0 U SOD/mg protein) and immunohistochemistry. CuZnSOD and MnSOD overexpression resulted in a significant radioprotection of HPLF compared to controls (surviving fraction [SF] ratio SOD/control > 1): CuZnSOD: 1.18-fold (95% confidence interval [CI]: 1.06-1.32; p = 0.005), MnSOD: 1.23-fold (95% CI: 1.07-1.43; p = 0.01). Conclusion: Overexpression of CuZnSOD and MnSOD in HPLF mediated an increase in clonogenic survival after irradiation compared to controls. In previous works, a lack of radioprotection in SOD-overexpressing tumor cells was observed. Therefore, the present results suggest that rAAV2 vectors are promising tools for the delivery of radioprotective genes in normal tissue. (orig.)

  1. Generation of insulin-producing human mesenchymal stem cells using recombinant adeno-associated virus.

    Science.gov (United States)

    Kim, Jeong Hwan; Park, Si-Nae; Suh, Hwal

    2007-02-28

    The purpose of current experiment is the generation of insulin-producing human mesenchymal stem cells as therapeutic source for the cure of type 1 diabetes. Type 1 diabetes is generally caused by insulin deficiency accompanied by the destruction of islet beta-cells. In various trials for the treatment of type 1 diabetes, cell-based gene therapy using stem cells is considered as one of the most useful candidate for the treatment. In this experiment, human mesenchymal stem cells were transduced with AAV which is containing furin-cleavable human preproinsulin gene to generate insulin-producing cells as surrogate beta-cells for the type 1 diabetes therapy. In the rAAV production procedure, rAAV was generated by transfection of AD293 cells. Human mesenchymal stems cells were transduced using rAAV with a various multiplicity of infection. Transduction of recombinant AAV was also tested using beta-galactosidse expression. Cell viability was determined by using MTT assay to evaluate the toxicity of the transduction procedure. Expression and production of Insulin were tested using reverse transcriptase-polymerase chain reaction and immunocytochemistry. Secretion of human insulin and C-peptide from the cells was assayed using enzyme-linked immunosorbent assay. Production of insulin and C-peptide from the test group represented a higher increase compared to the control group. In this study, we examined generation of insulin-producing cells from mesenchymal stem cells by genetic engineering for diabetes therapy. This work might be valuable to the field of tissue engineering for diabetes treatment.

  2. Overexpression of suppressor of cytokine signaling 3 in the arcuate nucleus of juvenile Phodopus sungorus alters seasonal body weight changes.

    Science.gov (United States)

    Ganjam, Goutham K; Benzler, Jonas; Pinkenburg, Olaf; Boucsein, Alisa; Stöhr, Sigrid; Steger, Juliane; Culmsee, Carsten; Barrett, Perry; Tups, Alexander

    2013-12-01

    The profound seasonal cycle in body weight exhibited by the Djungarian hamster (Phodopus sungorus) is associated with the development of hypothalamic leptin resistance during long day photoperiod (LD, 16:8 h light dark cycle), when body weight is elevated relative to short day photoperiod (SD, 8:16 h light dark cycle). We previously have shown that this seasonal change in physiology is associated with higher levels of mRNA for the potent inhibitor of leptin signaling, suppressor of cytokine signaling-3 (SOCS3), in the arcuate nucleus (ARC) of LD hamsters relative to hamsters in SD. The alteration in SOCS3 gene expression preceded the body weight change suggesting that SOCS3 might be the molecular switch of seasonal body weight changes. To functionally characterize the role of SOCS3 in seasonal body weight regulation, we injected SOCS3 expressing recombinant adeno-associated virus type-2 (rAAV2-SOCS3) constructs into the ARC of leptin sensitive SD hamsters immediately after weaning. Hamsters that received rAAV2 expressing enhanced green fluorescent protein (rAAV2-EGFP) served as controls. ARC-directed SOCS3 overexpression led to a significant increase in body weight over a period of 12 weeks without fully restoring the LD phenotype. This increase was partially due to elevated brown and white adipose tissue mass. Gene expression of pro-opiomelanocortin was increased while thyroid hormone converting enzyme DIO3 mRNA levels were reduced in SD hamsters with SOCS3 overexpression. In conclusion, our data suggest that ARC-directed SOCS3 overexpression partially overcomes the profound seasonal body weight cycle exhibited by the hamster which is associated with altered pro-opiomelanocortin and DIO3 gene expression.

  3. Phase 2 clinical trial of a recombinant adeno-associated viral vector expressing α1-antitrypsin: interim results.

    LENUS (Irish Health Repository)

    Flotte, Terence R

    2011-10-01

    Recombinant adeno-associated virus (rAAV) vectors offer promise for the gene therapy of α(1)-antitrypsin (AAT) deficiency. In our prior trial, an rAAV vector expressing human AAT (rAAV1-CB-hAAT) provided sustained, vector-derived AAT expression for >1 year. In the current phase 2 clinical trial, this same vector, produced by a herpes simplex virus complementation method, was administered to nine AAT-deficient individuals by intramuscular injection at doses of 6.0×10(11), 1.9×10(12), and 6.0×10(12) vector genomes\\/kg (n=3 subjects\\/dose). Vector-derived expression of normal (M-type) AAT in serum was dose dependent, peaked on day 30, and persisted for at least 90 days. Vector administration was well tolerated, with only mild injection site reactions and no serious adverse events. Serum creatine kinase was transiently elevated on day 30 in five of six subjects in the two higher dose groups and normalized by day 45. As expected, all subjects developed anti-AAV antibodies and interferon-γ enzyme-linked immunospot responses to AAV peptides, and no subjects developed antibodies to AAT. One subject in the mid-dose group developed T cell responses to a single AAT peptide unassociated with any clinical effects. Muscle biopsies obtained on day 90 showed strong immunostaining for AAT and moderate to marked inflammatory cell infiltrates composed primarily of CD3-reactive T lymphocytes that were primarily of the CD8(+) subtype. These results support the feasibility and safety of AAV gene therapy for AAT deficiency, and indicate that serum levels of vector-derived normal human AAT >20 μg\\/ml can be achieved. However, further improvements in the design or delivery of rAAV-AAT vectors will be required to achieve therapeutic target serum AAT concentrations.

  4. An amplified promoter system for targeted expression of calcium indicator proteins in the cerebellar cortex

    Directory of Open Access Journals (Sweden)

    Bernd eKuhn

    2012-07-01

    Full Text Available Recording of identified neuronal network activity using genetically encoded calcium indicators (GECIs requires labeling that is cell type-specific and bright enough for the detection of functional signals. However, specificity and strong expression are often not achievable using the same promoter. Here we present a combinatorial approach for targeted expression and single-cell-level quantification in which a weak promoter is used to drive trans-amplification under a strong general promoter. We demonstrated this approach using recombinant adeno-associated viruses (rAAVs to deliver the sequence of the GECI D3cpv in the mouse cerebellar cortex. Direct expression under the human synapsin promoter (hSYN led to high levels of expression (50-100 µM in five interneuron types of the cerebellar cortex but not in Purkinje cells (PCs (≤10 μM, yielding sufficient contrast to allow functional signals to be recorded from somata and processes in awake animals using two-photon microscopy. When the hSYN promoter was used to drive expression of the tetracycline transactivator (tTA, a second rAAV containing the bidirectional TET promoter (Ptetbi could drive strong D3cpv expression in PCs (10-300 µM, enough to allow reliable complex spike detection in the dendritic arbor. An amplified approach should be of use in monitoring neural processing in selected cell types and boosting expression of optogenetic probes. Additionally, we overcome cell toxicity associated with rAAV injection and/or local GECI overexpression by combining the virus injection with systemic pre-injection of hyperosmotic D-mannitol, and by this double the time window for functional imaging.

  5. Bcl-2–associated athanogene 3 protects the heart from ischemia/reperfusion injury

    Science.gov (United States)

    Su, Feifei; Myers, Valerie D.; Knezevic, Tijana; Wang, JuFang; Gao, Erhe; Madesh, Muniswamy; Tahrir, Farzaneh G.; Gupta, Manish K.; Gordon, Jennifer; Rabinowitz, Joseph; Tilley, Douglas G.; Khalili, Kamel; Cheung, Joseph Y.

    2016-01-01

    Bcl-2–associated athanogene 3 (BAG3) is an evolutionarily conserved protein expressed at high levels in the heart and the vasculature and in many cancers. While altered BAG3 expression has been associated with cardiac dysfunction, its role in ischemia/reperfusion (I/R) is unknown. To test the hypothesis that BAG3 protects the heart from reperfusion injury, in vivo cardiac function was measured in hearts infected with either recombinant adeno-associated virus serotype 9–expressing (rAAV9-expressing) BAG3 or GFP and subjected to I/R. To elucidate molecular mechanisms by which BAG3 protects against I/R injury, neonatal mouse ventricular cardiomyocytes (NMVCs) in which BAG3 levels were modified by adenovirus expressing (Ad-expressing) BAG3 or siBAG3 were exposed to hypoxia/reoxygenation (H/R). H/R significantly reduced NMVC BAG3 levels, which were associated with enhanced expression of apoptosis markers, decreased expression of autophagy markers, and reduced autophagy flux. The deleterious effects of H/R on apoptosis and autophagy were recapitulated by knockdown of BAG3 with Ad-siBAG3 and were rescued by Ad-BAG3. In vivo, treatment of mice with rAAV9-BAG3 prior to I/R significantly decreased infarct size and improved left ventricular function when compared with mice receiving rAAV9-GFP and improved markers of autophagy and apoptosis. These findings suggest that BAG3 may provide a therapeutic target in patients undergoing reperfusion after myocardial infarction. PMID:27882354

  6. The effect of surface demineralization of cortical bone allograft on the properties of recombinant adeno-associated virus coatings.

    Science.gov (United States)

    Yazici, Cemal; Yanoso, Laura; Xie, Chao; Reynolds, David G; Samulski, R Jude; Samulski, Jade; Yannariello-Brown, Judith; Gertzman, Arthur A; Zhang, Xinping; Awad, Hani A; Schwarz, Edward M

    2008-10-01

    Freeze-dried recombinant adeno-associated virus (rAAV) coated structural allografts have emerged as an approach to engender necrotic cortical bone with host factors that will persist for weeks following surgery to facilitate revascularization, osteointegration, and remodeling. However, one major limitation is the nonporous cortical surface that prohibits uniform distribution of the rAAV coating prior to freeze-drying. To overcome this we have developed a demineralization method to increase surface absorbance while retaining the structural integrity of the allograft. Demineralized bone wafers (DBW) made from human femoral allograft rings demonstrated a significant 21.1% (73.6+/-3.9% versus 52.5+/-2.6%; pcoating versus mineralized controls. Co-incubation of rAAV-luciferase (rAAV-Luc) coated DBW with a monolayer of C3H10T1/2 cells in culture led to peak luciferase levels that were not significantly different from soluble rAAV-Luc controls (p>0.05), although the peaks occurred at 60h and 12h, respectively. To assess the transduction efficiency of rAAV-Luc coated DBW in vivo, we first performed a dose response with allografts containing 10(7), 10(9) or 10(10) particles that were surgically implanted into the quadriceps of mice, and assayed by in vivo bioluminescence imaging (BLI) on days 1, 3, 5, 7, 10, 14, and 21. The results demonstrated a dose response in which the DBW coated with 10(10) rAAV-Luc particles achieved peak gene expression levels on day 3, which persisted until day 21, and was significantly greater than the 10(7) dose throughout this time period (pcoated with 10(10) rAAV-Luc particles failed to demonstrate any significant differences in transduction kinetics or efficiency in vivo. Thus, surface demineralization of human cortical bone allograft increases its absorbance for uniform rAAV coating, without affecting vector transduction efficiency.

  7. Striatal pre-enkephalin overexpression improves Huntington's disease symptoms in the R6/2 mouse model of Huntington's disease.

    Directory of Open Access Journals (Sweden)

    Stéphanie Bissonnette

    Full Text Available The reduction of pre-enkephalin (pENK mRNA expression might be an early sign of striatal neuronal dysfunction in Huntington's disease (HD, due to mutated huntingtin protein. Indeed, striatopallidal (pENK-containing neurodegeneration occurs at earlier stage of the disease, compare to the loss of striatonigral neurons. However, no data are available about the functional role of striatal pENK in HD. According to the neuroprotective properties of opioids that have been recognized recently, the objective of this study was to investigate whether striatal overexpression of pENK at early stage of HD can improve motor dysfunction, and/or reduce striatal neuronal loss in the R6/2 transgenic mouse model of HD. To achieve this goal recombinant adeno-associated-virus (rAAV2-containing green fluorescence protein (GFP-pENK was injected bilaterally in the striatum of R6/2 mice at 5 weeks old to overexpress opioid peptide pENK. Striatal injection of rAAV2-GFP was used as a control. Different behavioral tests were carried out before and/or after striatal injections of rAAV2. The animals were euthanized at 10 weeks old. Our results demonstrate that striatal overexpression of pENK had beneficial effects on behavioral symptoms of HD in R6/2 by: delaying the onset of decline in muscular force; reduction of clasping; improvement of fast motor activity, short-term memory and recognition; as well as normalization of anxiety-like behavior. The improvement of behavioral dysfunction in R6/2 mice having received rAAV2-GFP-pENK associated with upregulation of striatal pENK mRNA; the increased level of enkephalin peptide in the striatum, globus pallidus and substantia nigra; as well as the slight increase in the number of striatal neurons compared with other groups of R6/2. Accordingly, we suggest that at early stage of HD upregulation of striatal enkephalin might play a key role at attenuating illness symptoms.

  8. Sustained miRNA-mediated Knockdown of Mutant AAT With Simultaneous Augmentation of Wild-type AAT Has Minimal Effect on Global Liver miRNA Profiles

    OpenAIRE

    2013-01-01

    α-1 antitrypsin (AAT) deficiency can exhibit two pathologic states: a lung disease that is primarily due to the loss of AAT's antiprotease function, and a liver disease resulting from a toxic gain-of-function of the PiZ-AAT (Z-AAT) mutant protein. We have developed several recombinant adeno-associated virus (rAAV) vectors that incorporate microRNA (miRNA) sequences targeting the AAT gene while also driving the expression of miRNA-resistant wild-type AAT-PiM (M-AAT) gene, thus achieving concom...

  9. Closed-Loop Control of Vortex Formation in Separated Flows with Application to Micro Air Vehicles

    Science.gov (United States)

    2010-10-25

    to roppremon of disturbance» acting on the output of the closed loop Fatally. & can be interpreted as a feedforward function (Skngertad...aidividual coruroi I. be manuall ) «et by the incr The option» set by each mode are listed in Table 7 -f-.ttcmal Tngger" refer» to whether the timing...tnuurc vdocimevy wii the three -duncrruOBaJ charactowalioo of two-phawe tiowa’. .(eViomrirawn/ Srmice and r<raav>/cxi 13(51. 6«’ 694 IRL: Htp , J* dot

  10. Long-term outcomes of gene therapy for the treatment of Leber's hereditary optic neuropathy.

    Science.gov (United States)

    Yang, Shuo; Ma, Si-Qi; Wan, Xing; He, Heng; Pei, Han; Zhao, Min-Jian; Chen, Chen; Wang, Dao-Wen; Dong, Xiao-Yan; Yuan, Jia-Jia; Li, Bin

    2016-08-01

    Leber's hereditary optic neuropathy (LHON) is a disease that leads to blindness. Gene therapy has been investigated with some success, and could lead to important advancements in treating LHON. This was a prospective, open-label trial involving 9 LHON patients at Tongji Hospital, Wuhan, China, from August 2011 to December 2015. The purpose of this study was to evaluate the long-term outcomes of gene therapy for LHON. Nine LHON patients voluntarily received an intravitreal injection of rAAV2-ND4. Systemic examinations and visual function tests were performed during the 36-month follow-up period to determine the safety and efficacy of this gene therapy. Based on successful experiments in an animal model of LHON, 1 subject also received an rAAV2-ND4 injection in the second eye 12months after gene therapy was administered in the first eye. Recovery of visual acuity was defined as the primary outcome of this study. Changes in the visual field, visual evoked potential (VEP), optical coherence tomography findings, liver and kidney function, and antibodies against AAV2 were defined as secondary endpoints. Eight patients (Patients 2-9) received unilateral gene therapy and visual function improvement was observed in both treated eyes (Patients 4, 6, 7, and 8) and untreated eyes (Patients 2, 3, 4, 6 and 8). Visual regression fluctuations, defined as changes in visual acuity greater than or equal to 0.3 logMAR, were observed in Patients 2 and 9. Age at disease onset, disease duration, and the amount of remaining optic nerve fibers did not have a significant effect on the visual function improvement. The visual field and pattern reversal VEP also improved. The patient (Patient 1) who received gene therapy in both eyes had improved visual acuity in the injected eye after the first treatment. Unfortunately, visual acuity in this eye decreased 3months after he received gene therapy in the second eye. Animal experiments suggested that ND4 expression remains stable in the

  11. Long-term outcomes of gene therapy for the treatment of Leber's hereditary optic neuropathy

    Directory of Open Access Journals (Sweden)

    Shuo Yang

    2016-08-01

    Full Text Available Leber's hereditary optic neuropathy (LHON is a disease that leads to blindness. Gene therapy has been investigated with some success, and could lead to important advancements in treating LHON. This was a prospective, open-label trial involving 9 LHON patients at Tongji Hospital, Wuhan, China, from August 2011 to December 2015. The purpose of this study was to evaluate the long-term outcomes of gene therapy for LHON. Nine LHON patients voluntarily received an intravitreal injection of rAAV2-ND4. Systemic examinations and visual function tests were performed during the 36-month follow-up period to determine the safety and efficacy of this gene therapy. Based on successful experiments in an animal model of LHON, 1 subject also received an rAAV2-ND4 injection in the second eye 12 months after gene therapy was administered in the first eye. Recovery of visual acuity was defined as the primary outcome of this study. Changes in the visual field, visual evoked potential (VEP, optical coherence tomography findings, liver and kidney function, and antibodies against AAV2 were defined as secondary endpoints. Eight patients (Patients 2–9 received unilateral gene therapy and visual function improvement was observed in both treated eyes (Patients 4, 6, 7, and 8 and untreated eyes (Patients 2, 3, 4, 6 and 8. Visual regression fluctuations, defined as changes in visual acuity greater than or equal to 0.3 logMAR, were observed in Patients 2 and 9. Age at disease onset, disease duration, and the amount of remaining optic nerve fibers did not have a significant effect on the visual function improvement. The visual field and pattern reversal VEP also improved. The patient (Patient 1 who received gene therapy in both eyes had improved visual acuity in the injected eye after the first treatment. Unfortunately, visual acuity in this eye decreased 3 months after he received gene therapy in the second eye. Animal experiments suggested that ND4 expression remains

  12. Delivery and evaluation of recombinant adeno-associated viral vectors in the equine distal extremity for the treatment of laminitis.

    Science.gov (United States)

    Mason, J B; Gurda, B L; Van Wettere, A; Engiles, J B; Wilson, J M; Richardson, D W

    2017-01-01

    Our long-term aim is to develop a gene therapy approach for the prevention of laminitis in the contralateral foot of horses with major musculoskeletal injuries and non-weightbearing lameness. The goal of this study was to develop a practical method to efficiently deliver therapeutic proteins deep within the equine foot. Randomised in vivo experiment. We used recombinant adeno-associated viral vectors (rAAVs) to deliver marker genes using regional limb perfusion through the palmar digital artery of the horse. Vector serotypes rAAV2/1, 2/8 and 2/9 all successfully transduced equine foot tissues and displayed similar levels and patterns of transduction. The regional distribution of transduction within the foot decreased with decreasing vector dose. The highest transduction values were seen in the sole and coronary regions and the lowest transduction values were detected in the dorsal hoof-wall region. The use of a surfactant-enriched vector diluent increased regional distribution of the vector and improved the transduction in the hoof-wall region. The hoof-wall region of the foot, which exhibited the lowest levels of transduction using saline as the vector diluent, displayed a dramatic increase in transduction when surfactant was included in the vector diluent (9- to 81-fold increase). In transduced tissues, no significant difference was observed between promoters (chicken β-actin vs. cytomegalovirus) for gene expression. All horses tested for vector-neutralising antibodies were positive for serotype-specific neutralising antibodies to rAAV2/5. The current experiments demonstrate that transgenes can be successfully delivered to the equine distal extremity using rAAV vectors and that serotypes 2/8, 2/9 and 2/1 can successfully transduce tissues of the equine foot. When the vector was diluted with surfactant-containing saline, the level of transduction increased dramatically. The increased level of transduction due to the addition of surfactant also improved the

  13. Long-Term Effect of Gene Therapy on Leber's Congenital Amaurosis

    OpenAIRE

    Bainbridge, James W B; Mehat, Manjit S; Sundaram, Venki; Robbie, Scott J; Barker, Susie E; Ripamonti, Caterina; Georgiadis, Anastasios; Mowat, Freya M; Beattie, Stuart G; Gardner, Peter J; Feathers, Kecia L; Luong, Vy A; Yzer, Suzanne; Balaggan, Kamaljit; Viswanathan, Ananth

    2015-01-01

    Background Mutations in RPE65 cause Leber's congenital amaurosis, a progressive retinal degenerative disease that severely impairs sight in children. Gene therapy can result in modest improvements in night vision, but knowledge of its efficacy in humans is limited. Methods We performed a phase 1-2 open-label trial involving 12 participants to evaluate the safety and efficacy of gene therapy with a recombinant adeno-associated virus 2/2 (rAAV2/2) vector carrying the RPE65 complementary DNA, an...

  14. Bcl-2–associated athanogene 3 protects the heart from ischemia/reperfusion injury

    OpenAIRE

    Su, Feifei; Myers, Valerie D.; Knezevic, Tijana; Wang, JuFang; Gao, Erhe; Madesh, Muniswamy; Tahrir, Farzaneh G.; Gupta, Manish K.; Gordon, Jennifer; Rabinowitz, Joseph; Ramsey, Frederick V.; Tilley, Douglas G.; Khalili, Kamel; Cheung, Joseph Y.; Feldman, Arthur M.

    2016-01-01

    Bcl-2–associated athanogene 3 (BAG3) is an evolutionarily conserved protein expressed at high levels in the heart and the vasculature and in many cancers. While altered BAG3 expression has been associated with cardiac dysfunction, its role in ischemia/reperfusion (I/R) is unknown. To test the hypothesis that BAG3 protects the heart from reperfusion injury, in vivo cardiac function was measured in hearts infected with either recombinant adeno-associated virus serotype 9–expressing (rAAV9-expre...

  15. Four steps in the history of museum technologies and visitors' digital participation

    Directory of Open Access Journals (Sweden)

    Jørgen Riber Christensen

    2011-06-01

        In conclusion, the article asks where the place of signification or meaning of the exhibited object has moved to in the face of the increased degree of visitor participation. The tentative answer is that the signification generating process has moved away from the historical context of the object and towards the contemporary world of the visitor. The article connects this change in cultural discourse with Karin Sander's archaeological imagination and in a wider sense with the concept of negotiation from new historicism.

  16. Rejoinder

    OpenAIRE

    Dijkstra, Geske

    2006-01-01

    textabstractI want to begin by thanking Göran Holmqvist and Karin Metell Cueva for financing (on behalf of Sida) our independent research of the PRS processes in Latin America in the first place, and now in particular for taking up the challenge to respond to my doubts and criticisms of the PRS approach. They recognise that this approach has been too optimistic and, in particular, that too many goals have been attached to one single instrument. The proposal they put forward is interesting and...

  17. Uus kuum Eesti disain / Silvia Pärmann, Maris Takk

    Index Scriptorium Estoniae

    Pärmann, Silvia

    2015-01-01

    Karl Tauli disainitud taburet "Ämblu", Aap Piho puidust ümmargune laud, Marit Ilisoni tekkmantlid kollektsioonist "Longing for Sleep/Magada tahaks", Narma vaibakollektsioon "OPEN 2015/16" (disainerid Monika Järg, Kaidi Ploomipuu), Annike Laigo vaibakollektsioon "XX", Kairi Katmanni vaip "Storytellers", Valhalla Factory kollektsiooni "Daydreamers" ripptool, Raili Keivi betoonist ja portselanist nõudekollektsioon, Tarmo Luisu valgusti "Kassett", Stella Soomlaisi käekotid, Karin Kallase ja Erik Pasti (Stuudio Nahk) disainitud jalatsid, käekotid ja ehted, Kuula + Jylhä jalatsikollektsioon, Mokoko (disainer Mari Maripuu) nahast aksessuaarid

  18. Short outlines of books by Estonian authors / Rutt Hinrikus, Janika Kronberg

    Index Scriptorium Estoniae

    Hinrikus, Rutt, 1946-

    1996-01-01

    Kõiv, Madis. Studia memoriae I. Rännuaastad; Krull, Hasso. Luuletused 1987-1991; Krull, Hasso. Swinburne; Mihkelson, Ene. Nime vaev; Saarsen, Karin. Poola suvi; Traat, Mats. Hirm ja iha; Murutar, Kati. Naisena sündinud; Unt, Mati. Tere, kollane kass; Unt, Mati. Argimütoloogia sõnastik 1983-93; Unt, Mati. Vastne argimütoloogia; Mutt, Mihkel. Pingviin ja raisakass; Mutt, Mihkel. Rahvusvaheline mees; Teder, Tarmo. Kurat kargas pähe; Suuman, Sass. Targemat ei ole; Hirv, Indrek. Tähekerjus; Kaplinski, Jaan. Mitu suve ja kevadet; Kaplinski, Jaan. Jää ja Titanic

  19. Les parlements du peuple au Kenya

    DEFF Research Database (Denmark)

    Rasmussen, Jacob

    2012-01-01

    Despite the adoption of a new constitution in 2010, the post-election violence surrounding the flawed 2007 General Elections have fuelled on-going debates in Kenya about a state of political crisis and fragile democracy. Comparing two street parliaments from Eldoret and Nairobi in the context...... of electoral failure and constitutional reform, this paper investigates dynamics of political participation from below. The street parliaments form arenas for oral debates where speakers and participants collectively engage in the intentional shaping of spaces of speech. Inspired by the work of Karin Barber...

  20. First hoisting of the Portuguese flag at CERN

    CERN Multimedia

    Unknown, Unknown

    1986-01-01

    First hoisting of the Portuguese flag at CERN, following the country's membership. Photo taken on January 22 (or 23), 1986. From left to right: António Costa Lobo (Portugal's ambassador to the UN in Geneva), Gaspar Barreira, Karin Wall (José Mariano Gago's wife), José Mariano Gago (Portugal's JNICT president), Catarina Wall Gago (José Mariano Gago's daughter), João Varela, Herwig Schopper (CERN's Director-General), Eduardo Arantes e Oliveira (Portugal's Secretary of State for Science), , Margarida Nesbitt Rebelo, , Mário Pimenta, , Gustavo Castelo Branco, João Seixas, Sérgio Ramos, and Peter Sonderegger.

  1. Kui vana on kunstnik? / Anneli Porri

    Index Scriptorium Estoniae

    Porri, Anneli, 1980-

    2003-01-01

    Rahvusvahelise kunstihariduse konverentsi "InSea on Sea" raames Kunstiakadeemia galeriis Karin Laansoo kureeritud Tallinna Kunstikooli õpilaste tööde näitus "MÄRKmed", Draakoni galeriis Mari Sobolevi kureeritud Viljandi Maagümnaasiumi kunstistuudio näitus "Sisseastumiseksam maailma", rahvusraamatukogus Anneli Porri kureeritud näitus "Kokkuvõte" EKA tänavuste lõpetajate töödest ja näitus "Leitud tagahoovist", Kullo galeriis rahvusvaheline näitus "Dialoog erinevuste vahel"

  2. Elistagem eestimaist. Abikaasade meelevallas / Aita Kivi

    Index Scriptorium Estoniae

    Kivi, Aita, 1954-

    2002-01-01

    Sisu : Anton Nigov [Tõnu Õnnepalu]. Harjutused; Elme Väljaste. Kiusatus; Reet Kudu. Täiskuu ja tänavalatern; Vaapo Vaher. Surmakuul & seemnepurse; Jelena Skulskaja. Vene rulett; Inge Ojala. Asendusema ja olematud lapsed; Inge Ojala. Andesta - ma kasvasin tänaval; Barbara Vine. Korstnapühkija poeg; Charlotte Bingham. Suveaeg; Lisa Gardner. Täiuslik abikaasa; Wendy Northcutt. Darwini auhinnad 2; Dean Foster. Euroopa riikide etikett ja tavad; J. P. Donleavy. Naine, kellele meeldisid puhtad tualettruumid; Karin Altvegen. Kadunud hing; Grant Naylor. Punane kääbus; Dylan Evans. Emotsioon. Sentimenditeooria

  3. President tuli "koju" : President Toomas H. Ilves NY Eesti Majas

    Index Scriptorium Estoniae

    2007-01-01

    President Toomas Hendrik Ilvese ja proua Evelin Ilvese külaskäigust New Yorgi Eesti Majja 23. septembril 2007. Tervitussõnavõttudega esinesid New Yorgi Eesti Haridusseltsi esimees dr. Toomas Sõrra, New Yorgi Eesti Kooli juhataja Karin Kärner. Kirjaliku tervituse saatis presidendile tema omaaegne skaudijuht Emu Saarniit. President T.H. Ilves meenutas oma sõnavõtus, et esimest korda külastas ta New Yorgi Eesti Maja 50 aastat tagasi. Pärast ametlike sõnavõttude lõppu said kõik kohaletulnud isiklikult presidenti tervitada. Vabariigi President töövisiidil Ameerika Ühendriikides 20.-26.09.2007

  4. Documentation to the workshop 'Cluster in the environmental protection economy'; Dokumentation zum Workshop ''Cluster in der Umweltschutzwirtschaft''

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2009-12-11

    Within the workshop 'Cluster in the environmental protection economy' at the Umweltbundesamt (Dessau-Rosslau, Federal Republic of Germany) at 27th November, 2008, the following lectures were held: (a) Which contribution can cluster and cluster politics contribute to the promotion of the environmental protection economy? (Harald Legler); (b) Cluster in the environmental protection economy: Targets and expectations (Dieter Rehfeld); (c) Demands at the management of clusters (Karin Hoerhan); (d) Demands at the cluster politics in the environmental protection economy (Bernhard Hausberg); (e) Photovoltaics in Eastern Germany (Johann Wackenbauer); (f) Automotive industry in Bergisches Land (Thomas Lemken); (g) Competence centre environment Augsburg-Schwaben (Egon Beckord).

  5. The Role of I-kappa-B Kinases in Prostate Carcinogenesis and the Effect of Their Inhibition on Survival of Prostate Tumors

    Science.gov (United States)

    2007-01-01

    2. Karin, M., Y. Cao, F.R. Greten , and Z.W. Li. 2002. NF-kappaB in cancer: from innocent bystander to major culprit. Nat Rev Cancer 2:301-310. 3...transcriptional activity are regulated by p100. J Biol Chem 277:1405-1418. 6. Senftleben, U., Y. Cao, G. Xiao, F.R. Greten , G. Krahn, G. Bonizzi, Y...cells: evidence for the involvement of IKKalpha. Embo J 20:6805-6815. 8. Greten , F.R., L. Eckmann, T.F. Greten , J.M. Park, Z.W. Li, L.J. Egan, M.F

  6. "Neli arhitektuuri" : Eesti arhitektuurinäitusel Pariisis / Triin Ojari

    Index Scriptorium Estoniae

    Ojari, Triin, 1974-

    2000-01-01

    Baltimaade ja Rootsi arhitektuuribüroode töid tutvustav näitus galeriis La Galerie d'Architecture 22. X-10. XII 2000. Rootsist osales büroo White Arkitekter (arhitekt Mattias Lind), Lätist Arhis, Leedust Private Ideology, Eestist Kolm Pluss Üks (valiku tegi Karin Hallas). Kataloogi eessõna - läti arhitekt Janis Dripe. 2 ill. White Arkitekter (arhitektuuribüroo, Rootsi). Arhis (arhitektuuribüroo, Läti). Private Ideology (arhitektuuribüroo, Leedu)

  7. Die aantrekkingskrag van die see

    Directory of Open Access Journals (Sweden)

    I. Gertenbach

    2008-07-01

    This article compares the use of oceanic symbolism in the poetry of Karin Boye, Ingrid Jonker and Sylvia Plath. Freud’s “oceanic feeling” is described and contrasted with Jung’s theory of the “great mother”. According to Jung, the “great mother” is the unconscious which is represented by water. All these elements are discussed in the poems and compared to each other in order to gain a better understanding of the poetry. Although “death” (drowning in the ocean appears to point to new life, it ends in unification with the archetypal mother. This liberates the poets symbolically, but not physically.

  8. Sajand oodatud / Kristo Mäe

    Index Scriptorium Estoniae

    Mäe, Kristo

    2006-01-01

    Eesti Rahva Muuseumi uue hoone arhitektuurikonkursi võidutööst "Memory Field". Autorid: itaalia arhitekt Dan Dorell, liibanoni arhitekt Lina Ghotmeh ja jaapani arhitekt Tsuyoshi Tane. II preemia pälvis töö "Gems", autoriteks soome arhitektid Juho Grönholm, Antti Nousjoki, Janne Teräsvirta, Samuli Woolston, III preemia töö "Estonia is on the verge of a new millennium", autoriteks austria arhitektid Alfred Bramberger, Thomas Pucher, Heidrun Steinhauser, Martin Mathy, Christa Pucher. Krista Aru ja Karin Hallas-Murula arvamus, intervjuu Lina Ghotmet'iga

  9. Arhitektuuriuudised / Karen Jagodin

    Index Scriptorium Estoniae

    Jagodin, Karen, 1982-

    2006-01-01

    Elamurajoon Witbrant (Jacq de Brouwer, Erna van Sambeek) Tilburgis Lõuna-Hollandis. Kaubakonteineritest kokkuopandav reisimuuseum (Shigeru Ban). Säästlik, kerge, transporditav ja vähese materjalikuluga näidismaja (Richard Horden, saksa AB Haack + Höpfner). Ristikujuline maja (Bevk Perovic Arhitektid) Ljubljanas. Onnist inspireeritud pargikeskus (Chris Thurlbourne) Taani Rahvuspargis. Eestile ja muuseumidele pühendatud Soome arhitektuuriajakirja Arkkitehti 2006. a. 3. numbrist. Autoritest nimetatud Jorma Mukala, Pekka Vapaavuori, Leonhard Lapin, Triin Ojari, Tarja Nurmi (retsensioon Karin Hallas-Murula raamatule "Soome-Eesti : sajand arhitektuurisuhted)

  10. Kui seda mammit seal jalus ei oleks... / Indrek Pajo, Heiti Riisberg, Jaana Liigand... [jt.

    Index Scriptorium Estoniae

    2010-01-01

    Küsimusele, mida peaks noor keskjuht juhtimise osas tegema kui tunneb pärast paarikuist töötamist üha rohkem, et mõned tema alluvad, kes on temast oluliselt vanemad, töötavad talle vastu, vastavad endine Cleaner Ehitus vanemobjektijuht Indrek Pajo, Trigon Kapital pensioni- ja aktsiafondide eest hoolitsev Heiti Riisberg, konsultatsioonifirma Arikano omanik ja juht Jaana Liigand, Pharmadule OÜ personali- ja büroojuht Helen Neeve, Saku Õlletehase brändide Rock ja Carlsberg juht Karin Golubev ning Kaamos Kinnisvara juht Priit Uustulnd

  11. Maaülikooli uksed uutele tulijatele valla / Sirje Pärismaa

    Index Scriptorium Estoniae

    Pärismaa, Sirje, 1958-

    2008-01-01

    Oma õpingutest räägivad maaülikooli tudengid: Kadi Avi (majandusarvestus ja finantsjuhtimine III), Kaiti Pilk (maastikuarhitekt III), Mart Kelk (metsatööstuse magistrantuur I), Karin Ojamäe (rakendushüdrobioloogia III), Heidi Heitur (ökonoomika ja ettevõtlus I); Margus Paesalu (metsatööstus III), Kadri Kalle ( maastikukaitse ja -hoolduse magistrantuur I), Liisi Õunapuu (loodusvarade kasutamine ja kaitse III), Laura Niemelä (veterinaarmeditsiin IV) ja Peep Teppo (metsamajanduse magistrantuur II) ning õppeprorektor Jüri Lehtsaar

  12. Simultatenous determination of diacetyl and acetoin in traditional turkish butter stored in sheep’s rumen (Karinyagi

    Directory of Open Access Journals (Sweden)

    Gokce, R.

    2014-03-01

    Full Text Available Commercial Karinyagi (traditionally named karin is made of cows’ milk cream and is produced by filling butter in cleaned sheep’s rumen. The effect of butter storage in sheep’s rumen on the production of diacetyl and acetoin was investigated. These compounds were determined by GC-MS and they are the typical butter flavor commonly found in fermented dairy products. The modified method for the simultaneous extraction of diacetly and acetoin from butter samples was accurate and precise. The recoveries of diacetyl and acetoin were 94.7 and 110.8%, respectively, while the detection limits were 1.83 and 0.51 mg·L-1, respectively. The proposed method was applied for the monitoring of aroma compounds in Karin butter samples during different time intervals. The concentration of acetoin remained stable through 0–50 days while the concentration of diacetyl increased to 33.0 μg·g-1 up to 40 days and remained constant through 40–50 days.El Karinyagi comercial (nombre tradicional Karin está hecho de crema de leche de vaca, y producido llenando con mantequilla el rumen limpio de ovejas. Se ha investigado el efecto del almacenamiento de la mantequilla en el rumen de ovejas sobre la formación de diacetilo y acetoína. Estos compuestos son el típico flavor a mantequilla que se detecta comúnmente en los productos lácteos fermentados y han sido determinados mediante GC-MS. El método modificado para la extracción simultánea de diacetilo y acetoína en mantequilla resultó ser exacto y preciso y las recuperaciones de 94,7 y 110,8 % respectivamente, mientras que los límites de detección fueron 1.83 y 0,51 mg·L-1, respectivamente. El método propuesto se aplicó al control de compuestos aromáticos en Karin y muestras de mantequilla, durante diferentes intervalos de tiempo. La concentración de acetoína se mantuvo estable entre 0–50 días mientras que la concentración de diacetilo aumentó a 33,0 mg·g-1 hasta 40 días y se mantuvo constante

  13. Vaccinia virus as a subhelper for AAV replication and packaging

    Directory of Open Access Journals (Sweden)

    Andrea R Moore

    Full Text Available Adeno-associated virus (AAV has been widely used as a gene therapy vector to treat a variety of disorders. While these vectors are increasingly popular and successful in the clinic, there is still much to learn about the viruses. Understanding the biology of these viruses is essential in engineering better vectors and generating vectors more efficiently for large-scale use. AAV requires a helper for production and replication making this aspect of the viral life cycle crucial. Vaccinia virus (VV has been widely cited as a helper virus for AAV. However, to date, there are no detailed analyses of its helper function. Here, the helper role of VV was studied in detail. In contrast to common belief, we demonstrated that VV was not a sufficient helper virus for AAV replication. Vaccinia failed to produce rAAV and activate AAV promoters. While this virus could not support rAAV production, Vaccinia could initiate AAV replication and packaging when AAV promoter activation is not necessary. This activity is due to the ability of Vaccinia-driven Rep78 to transcribe in the cytoplasm and subsequently translate in the nucleus and undergo typical functions in the AAV life cycle. As such, VV is subhelper for AAV compared to complete helper functions of adenovirus.

  14. Gene Transfer Corrects Acute GM2 Gangliosidosis—Potential Therapeutic Contribution of Perivascular Enzyme Flow

    Science.gov (United States)

    Cachón-González, M Begoña; Wang, Susan Z; McNair, Rosamund; Bradley, Josephine; Lunn, David; Ziegler, Robin; Cheng, Seng H; Cox, Timothy M

    2012-01-01

    The GM2 gangliosidoses are fatal lysosomal storage diseases principally affecting the brain. Absence of β-hexosaminidase A and B activities in the Sandhoff mouse causes neurological dysfunction and recapitulates the acute Tay–Sachs (TSD) and Sandhoff diseases (SD) in infants. Intracranial coinjection of recombinant adeno-associated viral vectors (rAAV), serotype 2/1, expressing human β-hexosaminidase α (HEXA) and β (HEXB) subunits into 1-month-old Sandhoff mice gave unprecedented survival to 2 years and prevented disease throughout the brain and spinal cord. Classical manifestations of disease, including spasticity—as opposed to tremor-ataxia—were resolved by localized gene transfer to the striatum or cerebellum, respectively. Abundant biosynthesis of β-hexosaminidase isozymes and their global distribution via axonal, perivascular, and cerebrospinal fluid (CSF) spaces, as well as diffusion, account for the sustained phenotypic rescue—long-term protein expression by transduced brain parenchyma, choroid plexus epithelium, and dorsal root ganglia neurons supplies the corrective enzyme. Prolonged survival permitted expression of cryptic disease in organs not accessed by intracranial vector delivery. We contend that infusion of rAAV into CSF space and intraparenchymal administration by convection-enhanced delivery at a few strategic sites will optimally treat neurodegeneration in many diseases affecting the nervous system. PMID:22453766

  15. Gene delivery to skeletal muscle results in sustained expression and systemic delivery of a therapeutic protein.

    Science.gov (United States)

    Kessler, P D; Podsakoff, G M; Chen, X; McQuiston, S A; Colosi, P C; Matelis, L A; Kurtzman, G J; Byrne, B J

    1996-11-26

    Somatic gene therapy has been proposed as a means to achieve systemic delivery of therapeutic proteins. However, there is limited evidence that current methods of gene delivery can practically achieve this goal. In this study, we demonstrate that, following a single intramuscular administration of a recombinant adeno-associated virus (rAAV) vector containing the beta-galactosidase (AAV-lacZ) gene into adult BALB/c mice, protein expression was detected in myofibers for at least 32 weeks. A single intramuscular administration of an AAV vector containing a gene for human erythropoietin (AAV-Epo) into mice resulted in dose-dependent secretion of erythropoietin and corresponding increases in red blood cell production that persisted for up to 40 weeks. Primary human myotubes transduced in vitro with the AAV-Epo vector also showed dose-dependent production of Epo. These results demonstrate that rAAV vectors are able to transduce skeletal muscle and are capable of achieving sustained expression and systemic delivery of a therapeutic protein following a single intramuscular administration. Gene therapy using AAV vectors may provide a practical strategy for the treatment of inherited and acquired protein deficiencies.

  16. Gene delivery to skeletal muscle results in sustained expression and systemic delivery of a therapeutic protein

    Science.gov (United States)

    Kessler, Paul D.; Podsakoff, Gregory M.; Chen, Xiaojuan; McQuiston, Susan A.; Colosi, Peter C.; Matelis, Laura A.; Kurtzman, Gary J.; Byrne, Barry J.

    1996-01-01

    Somatic gene therapy has been proposed as a means to achieve systemic delivery of therapeutic proteins. However, there is limited evidence that current methods of gene delivery can practically achieve this goal. In this study, we demonstrate that, following a single intramuscular administration of a recombinant adeno-associated virus (rAAV) vector containing the β-galactosidase (AAV-lacZ) gene into adult BALB/c mice, protein expression was detected in myofibers for at least 32 weeks. A single intramuscular administration of an AAV vector containing a gene for human erythropoietin (AAV-Epo) into mice resulted in dose-dependent secretion of erythropoietin and corresponding increases in red blood cell production that persisted for up to 40 weeks. Primary human myotubes transduced in vitro with the AAV-Epo vector also showed dose-dependent production of Epo. These results demonstrate that rAAV vectors are able to transduce skeletal muscle and are capable of achieving sustained expression and systemic delivery of a therapeutic protein following a single intramuscular administration. Gene therapy using AAV vectors may provide a practical strategy for the treatment of inherited and acquired protein deficiencies. PMID:8943064

  17. A Baltic bio-bibliography - international lawyers of the Baltic region, past and present / Peter Macalister-Smith

    Index Scriptorium Estoniae

    Macalister-Smith, Peter

    2005-01-01

    Eestist: Küllike Jürimäe (s. 1962, Euroopa Ühenduste Kohtu esimese astme kohtu kohtunik alates 12. maist 2004) ; Uno Lõhmus (s. 1952, Euroopa Kohtu kohtunik alates 11. maist 2004) ; Rait Maruste (s. 1953, Euroopa Inimõiguste Kohtu kohtunik) ; Rein Müllerson (s. 1944, Institut de droit international liige, valiti aastal 1995) ; Ants Piip (1884-1942, rahvusvahelise õiguse uurija) ; Kaarel Robert Pusta (1883-1964, Institut de droit international liige, valiti aastal 1932) ; Otto Strandman (1875-1941, Permanent Court of International Justice - kohtunik 1938, 1939) ; Henn-Jüri Uibopuu (s. 1929, rahvusvahelise õiguse uurija) ; Abner T. Uustal (1915-1985, rahvusvahelise õiguse uurija). Lisatud: A note on F. F. Martens (1845-1909), lk. 159-164 (Friedrich Frommhold Martens, Frederic de Martens, Fjodor Fjodorovitsh Martens)

  18. Isolation, identification and characterization of lawsone from henna leaves powder with soxhlet technique

    Directory of Open Access Journals (Sweden)

    Mehrdad Mahkam

    2014-02-01

    Full Text Available Lawsone, a natural pigment present in the henna leaves, has been used as a skin and hair dye since 1400 BC. The concentration of this natural compound in leaves varies from place to place depending upon many of the environmental factors and the highest quantity reported so far is about 1% of the dry mass. Heretofore, it has been reported that natural colored extracts isolate from Henna leaves with many methods such as maceration, digestion, microwave and infusion. In this paper, regarding the therapeutic effects and traditional applications of henna, it was tried to isolate and characterize Lawsone from the henna leaves marketed in Tabriz city of Iran by soxhlet extraction technique in methanol solvent. The advantage of this technique is the isolation of large amounts of lawsone (720 mg from 40 g henne leaves powder with smaller quantity of methanol.

  19. Study of elastic scattering of polarized proton with 6He by folding model

    International Nuclear Information System (INIS)

    Iseri, Y.; Tanifuji, M.; Ishikawa, S.; Hiyama, E.; Yamamoto, Y.

    2005-01-01

    Experimental data of the elastic scattering of 6 He with polarized proton target has been analyzed using a simple folding model. As we regard 6 He as three bodies consisting of 4 He+n+n, the potential between the proton and 6 He is obtained by folding the two potentials, one between a proton and 4 He and another between a proton and a neutron, with the density distribution of 6 He. Calculated results of both the differential cross section and the vector analyzing power reproduce the experimental data satisfactorily. It is shown that the vector analyzing power of the p- 6 He scattering is mainly due to the spin orbit interaction between the proton and 4 He. (S. Funahashi)

  20. Beyond Depression: Towards a Process-Based Approach to Research, Diagnosis, and Treatment

    Science.gov (United States)

    Forgeard, Marie J. C.; Haigh, Emily A. P.; Beck, Aaron T.; Davidson, Richard J.; Henn, Fritz A.; Maier, Steven F.; Mayberg, Helen S.; Seligman, Martin E. P.

    2012-01-01

    Despite decades of research on the etiology and treatment of depression, a significant proportion of the population is affected by the disorder, fails to respond to treatment and is plagued by relapse. Six prominent scientists, Aaron Beck, Richard Davidson, Fritz Henn, Steven Maier, Helen Mayberg, and Martin Seligman, gathered to discuss the current state of scientific knowledge on depression, and in particular on the basic neurobiological and psychopathological processes at play in the disorder. These general themes were addressed: 1) the relevance of learned helplessness as a basic process involved in the development of depression; 2) the limitations of our current taxonomy of psychological disorders; 3) the need to work towards a psychobiological process-based taxonomy; and 4) the clinical implications of implementing such a process-based taxonomy. PMID:22509072