Training and Generalization of Peer-Directed Mands with Non-Vocal Children with Autism

Science.gov (United States)

Kodak, Tiffany; Paden, Amber; Dickes, Nitasha

2012-01-01

The current investigation evaluated the effects of extinction and prompts on training and generalization of peer-directed mands for preferred items using a picture exchange communication system with 2 children diagnosed with autism. Results showed that independent mands with a peer increased during treatment for both participants, generalized to a…

Assessing the acquisition and generalization of two mand forms with adults with severe developmental disabilities.

Science.gov (United States)

Chambers, Mark; Rehfeldt, Ruth Anne

2003-01-01

The purpose of this study was to determine whether manual sign or the Picture Exchange Communication System (P.E.C.S.) (Frost and Bondy, 1994) would be more effective in teaching mand skills to adults with mental retardation in the severe and profound range. Four participants were taught to mand for four reinforcing items using both communication modalities, in an alternating treatments design. Three of four participants demonstrated criterion performance across all four mands using P.E.C.S. first. Two of those three participants later demonstrated criterion performance for the mands using manual sign. The fourth participant was removed from the study during training due to illness, but her progress indicated greater acquisition with P.E.C.S. Generalization probes conducted at participants' respective residences showed that three participants demonstrated generalization across settings using P.E.C.S., and two participants demonstrated generalization across settings using manual sign. Participants were also more likely to mand for reinforcing items not present using P.E.C.S. than using manual sign.

Transferring Control of the Mand to the Motivating Operation in Children with Autism

Science.gov (United States)

Sweeney-Kerwin, Emily J.; Carbone, Vincent J.; O'Brien, Leigh; Zecchin, Gina; Janecky, Marietta N.

2007-01-01

Few studies have made use of B. F. Skinner's (1957) behavioral analysis of language and precise taxonomy of verbal behavior when describing the controlling variables for the mand relation. Consequently, the motivating operation (MO) has not typically been identified as an independent variable and the nature of a spontaneous mand has been…

Mands for information using "who?" and "which?" in the presence of establishing and abolishing operations.

Science.gov (United States)

Shillingsburg, M Alice; Bowen, Crystal N; Valentino, Amber L; Pierce, Laura E

2014-01-01

Treatments designed to teach mands for information have included prompting and differential reinforcement, as well as procedures to manipulate the relevant establishing operation (EO). However, previous studies have not included relevant abolishing operation (AO) conditions to ensure that the mand is under relevant antecedent control. Data on listener responses (i.e., use of the information) are also absent in the literature. The current study shows differential responding under EO and AO conditions and reports listener responses that demonstrate use of the provided information. Three participants, diagnosed with an autism spectrum disorder, learned to mand for information using "who?" and "which?" questions exclusively under EO conditions. In addition, each participant responded to the information provided to access a preferred item. Generalization of the "which?" mand for information was also demonstrated across novel stimuli. © Society for the Experimental Analysis of Behavior.

El conducto de Serres: alta prevalencia en mandíbulas infantiles

OpenAIRE

Hernández Huaripaucar, Edgar; Hernández, Liliana Gisela

2011-01-01

Objetivo. Conocer algunas características anátomo-topográficas del conducto de Serres y su prevalencia en mandíbulas infantiles, adultas y seniles. Material y método. La muestra estuvo constituida por 45 mandíbulas infantiles, adultas y seniles que presentaron o no el conducto de Serres, provenientes del anfiteatro de Anatomía de la Facultad de Odontología de la Universidad San Luís Gonzaga de Ica. La hipótesis operativa plantea que las características del conducto de Serres estudiad...

Mand falder - og rejser sig igen

DEFF Research Database (Denmark)

Evron, Lotte Orr

2016-01-01

Mand falder - og rejser sig igen Anmeldelse af Lotte Evron 6 stjerner Gennem et år følger Anne Wivel med kamera og samtaler sin mangeårige ven, Per Kirkebys, vej mod en ny hverdag efter et fald. Med venskabet som ramme opbygges et usædvanligt hudløst og respektfuldt portræt af Per Kirkeby – både...

MANIPULATION OF MOTIVATING OPERATIONS AND USE OF A SCRIPT-FADING PROCEDURE TO TEACH MANDS FOR LOCATION TO CHILDREN WITH LANGUAGE DELAYS

OpenAIRE

Howlett, Melissa A; Sidener, Tina M; Progar, Patrick R; Sidener, David W

2011-01-01

The effects of contriving motivating operations (MOs) and script fading on the acquisition of the mand “Where's [object]?” were evaluated in 2 boys with language delays. During each session, trials were alternated in which high-preference items were present (abolishing operation [AO] trials) or missing (establishing operation [EO] trials) from their typical locations. Both participants learned to mand during EO trials and not to mand during AO trials during training. Generalization of manding...

Effects of PECS Phase III Application Training on Independent Mands in Young Children with Autism

Science.gov (United States)

Love, Jessica June

2013-01-01

The purpose of this study was to examine the effects of PECS phase III application training on independent mands in young children with autism. Participants were five children with autism ranging from ages 2 to 4 years old. A multiple baseline across participants was used to evaluate acquisition of independent correct mands across baseline and…

Teaching Children with Autism to Engage in Peer-Directed Mands Using a Picture Exchange Communication System

Science.gov (United States)

Paden, Amber R.; Kodak, Tiffany; Fisher, Wayne W.; Gawley-Bullington, Elizabeth M.; Bouxsein, Kelly J.

2012-01-01

We evaluated differential reinforcement of alternative behavior (DRA) plus prompting to increase peer-directed mands for preferred items using a picture exchange communication system (PECS). Two nonvocal individuals with autism participated. Independent mands with a peer increased with the implementation of DRA plus prompting for both…

Mand træn din bækkenbund og få bedre rejsning

DEFF Research Database (Denmark)

Ravnkilde Marlet, Laila; Gerbild, Helle Nygaard

2016-01-01

Har du (eller din mand) problemer med at holde på urin eller at opnå eller bevare rejsningen, er der god grund til at søge hjælp. Der findes nemlig effektive og billige behandlingsmuligheder.......Har du (eller din mand) problemer med at holde på urin eller at opnå eller bevare rejsningen, er der god grund til at søge hjælp. Der findes nemlig effektive og billige behandlingsmuligheder....

Saints and Sainthood

DEFF Research Database (Denmark)

Bandak, Andreas

2018-01-01

Saints are central for personal devotion, as intercessors and mediators between humanity and God, and as models for emulation. Relationships with and images of saints and holy figures are by no means fixed, but have developed, mirrored and challenged different times and cultures. Sainthood is a p...... with him or her even if they never aspire to become a saint themselves. In this sense sainthood is a matter of social relationships—relationships which are perennially in the making....

Derived Manding in Children with Autism: Synthesizing Skinner's Verbal Behavior with Relational Frame Theory

Science.gov (United States)

Murphy, Carol; Barnes-Holmes, Dermot; Barnes-Holmes, Yvonne

2005-01-01

Mand functions for two stimuli (A1 and A2) were trained for 3 children with autism and were then incorporated into two related conditional discriminations (A1-B1/A2 -B2 and B1-C1/B2-C2). Tests were conducted to probe for a derived transfer of mand response functions from Al and A2 to C1 and C2, respectively. When 1 participant failed to…

Two African Saints in Medieval Germany.

Science.gov (United States)

Grimm, Reinhold

1992-01-01

The origin and development of two African saints are discussed: Saint Maurice, patron saint of the eastern empire of Otto I; and Caspar, the youngest of the three Magi. Their representation in German art is described and illustrated. (Author/LB)

Saints and Sainthood around the Baltic Sea

DEFF Research Database (Denmark)

This collection of articles covers most of the Baltic Region with a special focus on the cult of saints in Russia, Prussia, Finland, Sweden, Denmark, Estonia and Latvia (more commonly referred to in the Middle Ages as Livonia). The articles cover a wide range of topics, for example the introduction...... of foreign (and ‘old’) saints into new regions, the creation of new local cults of saints in newly Christianized regions, the role of the cult of saints in the creation of political and lay identities, the adaption of the cult of saints in folk poetry and the potential role of saints in times of war...

St. René: the Patron Saint of Anaesthetists and a Patron Saint of Canada.

Science.gov (United States)

Calverley, R K

1980-01-01

René Goupil, the Patron Saint of Anaesthetists, and a Patron Saint of Canada, was born in Angers, France in 1608 and studied surgery. He joined the Jesuits as a donné or volunteer worker in 1640 and served in the then tiny colony of Quebec as one of the first medical workers of Canada. After earning meritorious praise for his skills, he again volunteered to attend the Hurons at Sainte Marie, a mission far beyond the frontiers. René's canoe party was ambushed. He was captured by the Mohawks and endured eight weeks of cruel torture before being killed on September 29, 1642. René was the first of eight North American martyrs whose dedication was recognized by canonization in 1930. St. René was appointed Patron Saint of Anaesthetists in 1951.

EAARL-B Submerged Topography - Saint Croix and Saint Thomas, U.S. Virgin Islands, 2014

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Binary point-cloud data for part of the submerged environs of Saint Croix and Saint Thomas, U.S. Virgin Islands, were produced from remotely sensed, geographically...

Using Textual Prompts to Teach Mands for Information Using "Who?"

Science.gov (United States)

Shillingsburg, M. Alice; Gayman, Cassondra M.; Walton, William

2016-01-01

Recent research on teaching mands for information to children with language deficits has focused on manipulating establishing operations (EOs). However, only a few of those studies have programmed both EO conditions (in which information is needed) and abolishing operation (AO) conditions (in which information has already been provided) to ensure…

Église Saint-Pierre-Saint-Paul, Appoigny (Yonne

Directory of Open Access Journals (Sweden)

Fabrice Henrion

2009-09-01

Full Text Available L’église Saint-Pierre-Saint-Paul d’Appoigny témoigne d’un important chantier du début du XIIIe siècle , mais les sources permettent d’envisager la présence d’un sanctuaire au moins depuis l’extrême fin du VIe siècle. En effet, le règlement liturgique établit par Aunaire, évêque d’Auxerre de 561 à 604, mentionne Epponiacus dans la liste des sanctuaires auxerrois, sans que le vocable de l’église d’Appoigny ne soit précisé. Par ailleurs, depuis au moins le IXe siècle, une tradition, reprise par ...

75 FR 38718 - Safety Zone; Sault Sainte Marie 4th of July Fireworks, St. Mary's River, Sault Sainte Marie, MI

Science.gov (United States)

2010-07-06

... temporary safety zone on the St. Mary's River, Sault Sainte Marie, Michigan. This zone is intended to..., St. Mary's River, Sault Sainte Marie, MI (a) Location. The following area is a temporary safety zone...-AA00 Safety Zone; Sault Sainte Marie 4th of July Fireworks, St. Mary's River, Sault Sainte Marie, MI...

Tumor odontogênico adenomatóide em mandíbula Tumor odontógeno adenomatoide de la mandíbula Adenomatoid odontogenic tumor in mandible

Directory of Open Access Journals (Sweden)

Roberto de Almeida Azevedo

2011-06-01

Full Text Available Tumor odontogênico adenomatóide é uma lesão relativamente incomum, que acomete preferencialmente indivíduos do sexo feminino durante a segunda década de vida. Exibe como sítio de predileção a região anterior da maxila, é geralmente associado à coroa de um dente incluso. Este trabalho tem o objetivo de apresentar um caso clínico de tumor odontogênico adenomatóide. Este se apresenta localizado em região anterior da mandíbula. Pretende-se ainda abordar suas características clínicas, radiográficas e histológicas, além do tratamento cirúrgico conservador de eleição.El tumor odontógeno adenomatoide es una lesión relativamente infrecuente que afecta principalmente a las mujeres durante la segunda década de vida, porque su sitio predilecto es la región anterior de la mandíbula, con una lesión por lo general asociada con la corona del diente. Se reporta un caso de tumor odontógeno adenomatoide en la región anterior de la mandíbula junto con sus resultados clínicos, radiológicos e histológicos así como su tratamiento quirúrgico.Adenomatoid odontogenic tumor is a relatively uncommon lesion, which affects mainly individuals of the female during the second decade due, showing as a site of predilection for the anterior region of the maxilla presenting lesion usually associated with the crown of a tooth incluso. Os authors report a case of an adenomatoid odontogenic tumor in the anterior region of mandible, together with their clinical, radiographic and histological and its surgical treatment.

Establishing Derived Manding for Specific Amounts with Three Children: An Attempt at Synthesizing Skinner's "Verbal Behavior" with Relational Frame Theory

Science.gov (United States)

Murphy, Carol; Barnes-Holmes, Dermot

2009-01-01

Participants were 2 typically developing children, aged 9 and 10 years, and 1 child, aged 4 years, with a reported severe speech delay. Five specific mand functions were trained such that participants learned to mand for the delivery or removal of tokens to the value of -2, -1, 0, +1, and +2, by presenting an arbitrary stimulus (A1, A2, A3, A4,…

Mand falder – og rejser sig igen på Faldnørden

DEFF Research Database (Denmark)

Evron, Lotte Orr

2017-01-01

Denne blogpost indeholder en filmanmeldelse af Anne Wivels bevægende film: Mand falder, om multikunstneren Per Kirkebys fald og tilbagevenden til kunsten og livet. Filmen gjorde stort indtryk på mig og viser det eksistentielle perspektiv i forbindelse med et fatalt fald meget konkret. Anmeldelsen...

Saint: a lightweight integration environment for model annotation.

Science.gov (United States)

Lister, Allyson L; Pocock, Matthew; Taschuk, Morgan; Wipat, Anil

2009-11-15

Saint is a web application which provides a lightweight annotation integration environment for quantitative biological models. The system enables modellers to rapidly mark up models with biological information derived from a range of data sources. Saint is freely available for use on the web at http://www.cisban.ac.uk/saint. The web application is implemented in Google Web Toolkit and Tomcat, with all major browsers supported. The Java source code is freely available for download at http://saint-annotate.sourceforge.net. The Saint web server requires an installation of libSBML and has been tested on Linux (32-bit Ubuntu 8.10 and 9.04).

The features of Saint Louis

Directory of Open Access Journals (Sweden)

Pierre-Yves Le Pogam

2017-12-01

Full Text Available The recent ninth centenary of the birth of Saint Louis, in 2014, gave the possibility to renew the study of the man and his reign, so important for French history, not in a nationalistic agenda or for a mere celebration, but in order to shed new light on a figure indeed profoundly studied but deserving a repeated attention, so much Louis IX lends to many-sided, even contradictory explanations. In the past, one has paid an almost maniacal attention to the point of the physical appearance of the king, looking in some images for a reflection of, or indeed a portrait of Saint Louis. Or, on the contrary and more recently, one has denied any validity to this trend of studies. In line with the essay I devoted on this question in the catalogue of the recent exhibition on Saint Louis in Paris, I would like here to go further in the analysis, especially regarding the meaning of details of the dress and bodily features of the saint king.

Gateway to the Syriac Saints: A Database Project

Directory of Open Access Journals (Sweden)

Jeanne-Nicole Mellon Saint-Laurent

2016-04-01

Full Text Available This article describes The Gateway to the Syriac Saints, a database project developed by the Syriac Reference Portal (www.syriaca.org. It is a research tool for the study of Syriac saints and hagiographic texts. The Gateway to the Syriac Saints is a two-volume database: 1 Qadishe and 2 Bibliotheca Hagiographica Syriaca Electronica (BHSE. Hagiography, the lives of the saints, is a multiform genre. It contains elements of myth, history, biblical exegesis, romance, and theology. The production of saints’ lives blossomed in late antiquity alongside the growth of the cult of the saints. Scholars have attended to hagiographic traditions in Greek and Latin, but many scholars have yet to discover the richness of Syriac hagiographic literature: the stories, homilies, and hymns on the saints that Christians of the Middle East told and preserved. It is our hope that our database will give scholars and students increased access to these traditions to generate new scholarship. The first volume, Qadishe or “saints” in Syriac, is a digital catalogue of saints or holy persons venerated in the Syriac tradition. Some saints are native to the Syriac-speaking milieu, whereas others come from other linguistic or cultural traditions. Through the translation of their hagiographies and the diffusion of saints’ cults in the late antique world, saints were adopted, “imported,” and appropriated into Syriac religious memory. The second volume, the BHSE, focuses on Syriac hagiographic texts. The BHSE contains the titles of over 1000 Syriac stories, hymns, and homilies on saints. It also includes authors’ or hagiographers’ names, the first and last lines of the texts (in Syriac, English, and French, bibliographic information, and the names of the manuscripts containing these hagiographic works. We have also listed modern and ancient translations of these works. All of the data in the Gateway to the Syriac Saints has been encoded in TEI, and it is fully

Investigating the Acquisition, Generalization, and Emergence of Untrained Verbal Operants for Mands Acquired Using the Picture Exchange Communication System in Adults with Severe Developmental Disabilities

Science.gov (United States)

Ziomek, M. M.; Rehfeldt, R. A.

2008-01-01

This study compared the total amount of training time and total number of trial blocks for individuals with severe developmental disabilities to acquire mands under control of unconditioned establishing operations and mands under control of transitive conditioned establishing operations for manual sign and for the Picture Exchange Communication…

Saint rulers as a category of sanctity in Orthodoxy

Directory of Open Access Journals (Sweden)

Jarosław Charkiewicz

2015-11-01

Full Text Available In the wide spectre of Orthodox human sanctity, among others, there is also a category of saint rulers. Despite the fact that it’s represented by a significant number of saints, it remains, utterly undeservedly, in the shadow of the others – more known or more deeply rooted historically. On top of that, the situation may result as well from the fact that saint rulers, as a distinct category of saint, are not separately mentioned neither during the proskomedia, nor during the intercessory prayer of the anaphora. Still, the saint rulers definitely should be considered a separate type of sanctity, deserving a somehow wider presentation. Such is therefore the aim of this article.It is also an attempt of suggesting an interior systematic of this category of sanctity. Preserving, appearing in science, classifications of the saint rulers, the author gives also his own proposition. In the category of saint rulers there are four following groups: (1 rulerswho weren’t neither martyrs nor monks or passion-bearers, (2 rulers who died martyrs, (3 rulers who long before their death became monks and led monastic life, (4 rulers-passion-bearers.

Saint rulers as a category of sanctity in Orthodoxy

OpenAIRE

Jarosław Charkiewicz

2015-01-01

In the wide spectre of Orthodox human sanctity, among others, there is also a category of saint rulers. Despite the fact that it’s represented by a significant number of saints, it remains, utterly undeservedly, in the shadow of the others – more known or more deeply rooted historically. On top of that, the situation may result as well from the fact that saint rulers, as a distinct category of saint, are not separately mentioned neither during the proskomedia, nor during the intercessory pray...

A "Sacra Rappresentazione" of Saint Apollonia's Martyrdom.

Science.gov (United States)

Eramo, Stefano; Natali, Alessio; Bravi, Monia; Cella, Diana; Milia, Egle

The literary sources of Saint Apollonia's martyrdom and the evolution of Medieval and Renaissance religious drama are presented along with the compendium of a "Sacra Rappresentazione" from Italian Renaissance regarding Saint Apollonia's Martyrdom. Copyright American Academy of the History of Dentistry.

Uso de la morfometría de las mandíbulas de cefalópodos en estudios de contenido estomacal

OpenAIRE

Vega,Marco Antonio

2011-01-01

La sistemática y taxonomía de cefalópodos del mar de Chile, sigue siendo escasa e incompleta. Muchas especies, raramente son capturadas por métodos convencionales, y regularmente son colectadas de estómagos de depredadores. La identificación en los estómagos se realiza, con frecuencia, mediante sus mandíbulas o picos córneos. En nuestra costa estas estructuras, en general, son poco utilizadas con fines taxonómicos. Este estudio describe y analiza preliminarmente la morfometría de 111 mandíbul...

REPRESENTATION OF THE SAINTS IN ORTHODOX ICONOGRAPHY

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Veronica GHEORGHIȚĂ

2014-06-01

Full Text Available In Romanian iconography, the icon is described as an image of truth, expressing, strengthening and teaching the truth of orthodox faith. Adept and guardian of the byzantine style, the icon depicts the saints in their divine and human nature, but has traits that show the holiness, perfection and simplicity of their life. Given that the saints have been seen as modest, abstinent, fasting people, they have never been portrayed as overweight, but thin, with hollow cheeks and a frail physical appearance. In fact, the iconographic representations are characterized by a high ability to summarize the reality, the physical appearance of the saints being non-anatomical, disproportionate. Lately, it has been noticed in certain iconographic representations a change in painting style: to reproduce reality as faithfully as possible. The orientation toward the icon's external beauty involved the redefinition of the iconic portrait of the saints, who are depicted as human as possible.

DeMand: A tool for evaluating and comparing device-level demand and supply forecast models

DEFF Research Database (Denmark)

Neupane, Bijay; Siksnys, Laurynas; Pedersen, Torben Bach

2016-01-01

Fine-grained device-level predictions of both shiftable and non-shiftable energy demand and supply is vital in order to take advantage of Demand Response (DR) for efficient utilization of Renewable Energy Sources. The selection of an effective device-level load forecast model is a challenging task......, mainly due to the diversity of the models and the lack of proper tools and datasets that can be used to validate them. In this paper, we introduce the DeMand system for fine-tuning, analyzing, and validating the device-level forecast models. The system offers several built-in device-level measurement...... datasets, forecast models, features, and errors measures, thus semi-automating most of the steps of the forecast model selection and validation process. This paper presents the architecture and data model of the DeMand system; and provides a use-case example on how one particular forecast model...

René Goupil: patron saint of anesthetists.

Science.gov (United States)

Quintal, J

1995-06-01

René Goupil, the patron saint of anesthetists, is addressed mainly as a 17th century French surgeon who practiced his art in North America. He is also presented as a Jesuit martyr and saint. The article freely borrows from past writings of Jesuits on René Goupil.

Prevalence of ciguatoxins in lionfish (Pterois spp.) from Guadeloupe, Saint Martin, and Saint Barthélmy Islands (Caribbean).

Science.gov (United States)

Soliño, Lucía; Widgy, Saha; Pautonnier, Anthony; Turquet, Jean; Loeffler, Christopher R; Flores Quintana, Harold A; Diogène, Jorge

2015-08-01

Lionfish (Pterois spp.) are invasive species that have recently spread throughout the Caribbean. Lionfish are available for purchase in local markets for human consumption in several islands of the region. We examined the prevalence of ciguatoxins (CTXs) in lionfish from the French Antilles, a ciguatera-endemic region. The neuroblastoma-2a (N2a) cell assay was used to assess composite cytotoxicity in 120 fish samples collected from the surrounding waters of Guadeloupe (n = 60), Saint Barthélemy Islands (n = 55) and Saint Martin (n = 5). Twenty-seven of these samples exhibited CTX-like activity by the N2a assay. Ciguatoxin (CTX) was confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in multiple samples that presented highest composite toxicity levels by N2a. Those fish found to contain CTXs were all from Saint Barthélemy. Lionfish from Guadeloupe and Saint Martin did not exhibit toxin activity, although the sample size from Saint Martin was insufficient to draw any conclusions as to the incidence of CTXs. In this study, we provide information about the potential hazard of ciguatera associated with the consumption of lionfish from known endemic areas. We also demonstrate the utility of the cell-based assay combined with LC-MS/MS to assess activity and to provide structural confirmation of CTXs respectively. Copyright © 2015 Elsevier Ltd. All rights reserved.

African Journals Online: Saint Kitts and Nevis

African Journals Online (AJOL)

African Journals Online: Saint Kitts and Nevis. Home > African Journals Online: Saint Kitts and Nevis. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register · Browse By Category · Browse Alphabetically · Browse By Country · List All Titles · Free to read Titles This Journal is ...

Making midwives: postmodern conditions and midwifery training in Saint Lucia.

Science.gov (United States)

Hsu, C

2001-01-01

Drawing on material from fieldwork conducted on the island of Saint Lucia, I examine how Saint Lucian nurse-midwives and student midwives negotiate multiple ways of understanding and evaluating their practices and roles in light of contradictory and powerful cultural, historical, and political forces. I argue that, although Saint Lucian nurse-midwives may not qualify as "postmodern" according to the criteria proposed by Davis-Floyd and Davis (1996), they are nonetheless struggling with postmodern conditions as they negotiate between competing healing ideologies. I illustrate the significance of these negotiations through analyzing: (1) the ways nurse-midwives understand and articulate the healing ideologies at play in Saint Lucia, (2) historical and ideological aspects of the Saint Lucian nurse-midwifery training program, and (3) a classroom discussion during which student reported on "bush-midwives."

Saint Benedict of Palermo in Spain

Directory of Open Access Journals (Sweden)

Bernard VINCENT

2016-06-01

Full Text Available Among the devotions to the black Saints, the one of which Benedict of Palermo was the object in Spain in the 17th and 18th centuries was the most remarkable. Born near Palermo to African slaves, this lay brother of a Sicilian franciscan convent, died in the odor of sanctity in 1589. His immediate popularity, encouraged both by the Church and by the Hispanic monarchy, was considerable within the communities of slaves and free blacks. Benedict was beatified in 1743, event which was followed by numerous celebrations and his eventual canonization in 1807. The fervour which surrounded him fade gradually because of the competition represented by the devotion to other black saints and by the very visible decline of slavery. However, thanks to the efforts of the franciscan order, the worship of Saint Benoît of Palermo continued until our days, particularly in Galicia.

ALICE takes root in Saint-Genis-Pouilly

CERN Multimedia

Patrice Loiez

2004-01-01

To celebrate the CERN 50th anniversary and to emphasize the close ties between the community of Saint-Genis-Pouilly, CERN and the ALICE Collaboration, Hubert Bertrand, Mayor of Saint-Genis-Pouilly and Christian Fabjan, Technical Coordinator of the ALICE Experiment, planted a tree on Saturday 16 October 2004 in front of the Jean Monet Culture Center.

ALICE takes root in Saint-Genis-Pouilly

CERN Multimedia

2004-01-01

To celebrate the CERN 50th anniversary and to emphasize the close ties between the community of Saint-Genis-Pouilly, CERN and the ALICE Collaboration, Hubert Bertrand, Mayor of Saint-Genis-Pouilly and Christian Fabjan, Technical Coordinator of the ALICE Experiment, planted a tree on Saturday 16 October 2004 in front of the Jean Monet Culture Center.

The Effects of Parent-Implemented PECS Training on Improvisation of Mands by Children with Autism

Science.gov (United States)

Chaabane, Delia B. Ben; Alber-Morgan, Sheila R.; DeBar, Ruth M.

2009-01-01

The present study examined the extent to which mothers were able to train their children, 2 boys with autism, to exchange novel pictures to request items using the picture exchange communication system (PECS). Generalization probes assessing each child's ability to mand for untrained items were conducted throughout conditions. Using a multiple…

The church Saint-Symphorien in Nuits-Saint-Georges. An architectural and decorative syncretism, ca 1220-1240

Directory of Open Access Journals (Sweden)

Sylvain Demarthe

2011-12-01

Full Text Available Saint Symphorian’s church in Nuits-Saint-Georges (Burgundy, which dates from near 1220-1240, is situated during the transition of two architectural styles and attests a local but rather backward-looking Cistercian legacy that some Gothic urban and more innovative elements modernize. Moreover the church takes part, for the same reasons as those in Agencourt, Gerland and Prissey, in the surprising wave of reconstructions which denotes among others the important economic development of the country of Cîteaux at the beginning of the 13th century.

Who is Jesus Christ for the Ruthenian and Russian Saints? A Theological Exploration of St Dimitry Rostovsky’s The Lives of the Saints

Directory of Open Access Journals (Sweden)

Krzysztof Leśniewski

2014-12-01

Full Text Available Christian hagiography reveals not only the facts of life of individual saints, but first of all, it is a description of theexperience of God loving mankind. From the beginning of Christianity, of great importance in building faith, hope andlove had had stories about those believers who responded with a particular radicalism for the prevenient love of God inthe Trinity of Persons. These stories have been written in various forms of literature.Author in the introductory part of the article introduces into the lives of the saints and their role in the Ruthenian andRussian Orthodoxy. Essentially he focuses on presenting The Menaia edited by St Dimitry Rostovski. This book waspublished in the years 1711 to 1718 in Old Church Slavonic. At the beginning of the twentieth century, it has been rewrittenand translated into Russian. This monumental work appeared in the years 1903-1911 under the title The Lives of theSaints. It has been a rich source of information for researchers from many disciplines.From a theological point of view, the author studied The Lives of the Saints in order to find direct references to theperson and teaching of Jesus Christ. For this purpose, he analyzed quotations from the New Testament, especially fromthe Gospel, referring directly to the Person and teaching of Jesus Christ, as well as the prayers addressed to Him. In hisanalysis, he searched for answers to the questions: Who is Jesus Christ for the Ruthenian and Russian saints, and whatkind of His images predominate in The Lives of the Saints.The first part of the article, on the basis of source texts, indicates personal qualities of Jesus Christ. The Savior hasbeen experienced by the Ruthenian and Russian saints as God who is close to man, the blessed King, the best Lord, theComforter of the crying, the Light, the merciful God, and the Foundation of all good. The image of Christ as sinless,innocent suffering and merciful shines especially in the devotion of Saints Passion

Screening of "Particule Fever" at Saint-Genis

CERN Multimedia

Brice, Maximilien

2015-01-01

La Mission Permanente de la France auprès des Nations Unies organise une projection gratuite du film "Particle Fever" jeudi 19 février 2015 à 19h30 au théâtre du Bordeau de Saint-Genis-Pouilly. Ce partenariat avec le CERN et la Ville de Saint-Genis-Pouilly permet de comprendre, au travers de ce film captivant, l'activité du CERN.

The sacred disease and its patron saint.

Science.gov (United States)

Fatović-Ferencić, S; Dürrigl, M A

2001-08-01

Although the Hippocratic natural theory of epilepsy as a brain disorder originated around 400 bc , it did not begin to take root until the 18th and 19th centuries, leaving the intervening centuries dominated by mostly supernatural concepts. This article provides historical insight into human behavior when afflicted with disease: supplication to a patron saint, Saint Valentine, a cult that spread throughout Europe.

Hemoglobin C disease

Science.gov (United States)

Clinical hemoglobin C ... Hemoglobin C is an abnormal type of hemoglobin, the protein in red blood cells that carries oxygen. It is ... Americans. You are more likely to have hemoglobin C disease if someone in your family has had ...

UNDER THE PROTECTION OF THE GOD'S SAINT. DYKANKA IMAGE OF SAINT NICHOLAS THE WONDER-WORKER AND ITS ROLE IN NIKOLAI GOGOL'S LIFE

Directory of Open Access Journals (Sweden)

Voropaev V. A.

2008-11-01

Full Text Available The article tells the history of St. Nicholas Church in Dykanka and its holy image of Saint Nicholas, examines some details of the Gogol family life, the legend of Maria Gogol-Yanovskaya's marriage and her oath in front of the Holy Saint icon.

The history of the missal directions of the All Saints Solemnity

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Jan Szczych

2009-09-01

Full Text Available The cult of the saints had its beginning in the Christian ancient times. Since then it was transformed in its own celebrations in honour of All the Saints. The official liturgy of the feast-day of Omnium Sanctorum (All Saints was stabilized in close relation to the development of collective worship of the saints in the West.  The historical liturgical witnesses from the Middle Ages and of the Trident Council time demonstrate a progressive and very natural process of establishment the missal texts of this liturgical celebration. The form of some liturgical directions in the current Missale Romanum (Latin Missal, unchanged for ages, confirms the continuity and constancy of this celebration in the history of Catholic Church. These missal directions explicitly show the same idea of celebration and timeless meaning of the All Saints Solemnity.

The results of virological surveillance for intrauterine infections in Saint-Petersburg

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E. A. Murina

2014-01-01

Full Text Available The purpose of researchis the determiningthe etiological structure ofintrauterine infections in Saint-Petersburg pediatric patients, pregnant women andinfants born to them using a variety of virological methods.Methods: serum from 164 children aged from 1 month to 14 years with diagnosis of «intrauterine infection». Serum from 80 pregnant women, collected in each trimester (total – 240 samples, their 42 children (at the age of 1–2 and 4–6  months of life, total – 82 samples. Immunoglobulin Mand G (IgM and IgGto herpes virus type 1, cytomegalovirus (CMV, Toxoplasma gondii, mycoplasma and chlamydia, rubella, Epstein-Barr virus (EBV and parvovirus B19, as well as IgG avidity,were determined by ELISA in all these samples. Theimmunoblot (Western blot, using the «Immunoblot2000» with test kits from «Euroimmun AG» (Germany, was applied to confirm cases. Statistical analysis wasperformed with theprograms Microsoft Excel, Statistica6.Results: cytomegalovirus, herpes virus 1st type and Epstein-Barr virus infections are dominate in the structure of intrauterine ones (45%, 23% and 14%, respectively. Laboratory evidence ofreactivationof cytomegalovirus (35% ofpregnant womenin the 2nd and/or 3rd trimesters and acuteparvovirus infection (15% of cases were found. Specific IgM to cytomegalovirus were detected in 6,2% ofchildren in the firstsix months of life.Conclusions: with the aim of early detection of cytomegalovirus reactivation and acute parvovirus infection it isnecessary to monitor pregnant women with the definition of specific IgM, IgG and avidity IgG. The procedure to using immunoblotting in the diagnosis of intrauterine infectionsneeds to be further study.

Energy Transition Initiative: Island Energy Snapshot - Saint Martin/Sint Maarten

Energy Technology Data Exchange (ETDEWEB)

2015-09-01

This profile provides a snapshot of the energy landscape of the northeast Caribbean island Saint Martin. The island is divided between two nations, France in the north (Saint-Martin) and the Netherlands in the south (Sint Maarten).

Variación en la forma de la mandíbula en Monophyllus redmani (Chiroptera: Phyllostomidae): análisis geométrico de la variación sexual y geográfica

OpenAIRE

Mancina, Carlos A.; Balseiro, Fernando

2010-01-01

Analizamos la variación sexual y geográfica en la forma de la mandíbula de Monophyllus redmani mediante métodos de morfometría geométrica. Las diferencias en el tamaño y la forma de la mandíbula fueron examinadas usando 10 "landmarks" o puntos de referencia sobre la vista lateral de la mandíbula de 69 individuos. La significación de la variación de la forma entre sexos y regiones geográficas fue analizada mediante un análisis múltiple de varianza de dos vías sobre la matriz de las variables d...

The effects of mands and models on the speech of unresponsive language-delayed preschool children.

Science.gov (United States)

Warren, S F; McQuarter, R J; Rogers-Warren, A K

1984-02-01

The effects of the systematic use of mands (non-yes/no questions and instructions to verbalize), models (imitative prompts), and specific consequent events on the productive verbal behavior of three unresponsive, socially isolate, language-delayed preschool children were investigated in a multiple-baseline design within a classroom free play period. Following a lengthy intervention condition, experimental procedures were systematically faded out to check for maintenance effects. The treatment resulted in increases in total verbalizations and nonobligatory speech (initiations) by the subjects. Subjects also became more responsive in obligatory speech situations. In a second free play (generalization) setting, increased rates of total child verbalizations and nonobligatory verbalizations were observed for all three subjects, and two of the three subjects were more responsive compared to their baselines in the first free play setting. Rate of total teacher verbalizations and questions were also higher in this setting. Maintenance of the treatment effects was shown during the fading condition in the intervention setting. The subjects' MLUs (mean length of utterance) increased during the intervention condition when the teacher began prompting a minimum of two-word utterances in response to a mand or model.

The effect of heart-and kidney-Benefiting Chinese Herbal Medicine on the function of cholinergic M-and Gamma amino-butyric acid (GABA) receptor in brain tissues of analogue dementia rats

International Nuclear Information System (INIS)

Mo Qizhong; Gong Bin; Fang Jun

1993-01-01

3 H-QNB and 3 H-GABA were used as radioactive ligand for M-and GABA receptors respectively. M-and GABA receptors were assayed by radioligand binding assay (RBA) in cerebral cortex, hippocampus and cerebellum of analogue dementia rats. It was found that R t of M receptor was decreased in cerebral cortex and hippocampus and R t of GABA receptor was decreased in cerebellum of analogue dementia rats. The dissociation constant (K D ) of M-receptor was decreased significantly in cerebral cortex and K D value of (GABA) receptor was decreased in cerebellum of analogue dementia rats. The decreased R t of M-and GABA receptor in brain tissue of analogue dementia rats was raised by Heart- and Kidney-Benefiting Chinese Herbs as well as hydergin

The Picture Exchange Communication System: Effects on Manding and Speech Development for School-Aged Children with Autism

Science.gov (United States)

Tincani, Matt; Crozier, Shannon; Alazett, Shannon

2006-01-01

We examined the effects of the Picture Exchange Communication System (PECS; Frost & Bondy, 2002) on the manding (requesting) and speech development of school-aged children with autism. In study 1, two participants, Damian and Bob, were taught PECS within a delayed multiple baseline design. Both participants demonstrated increased levels of manding…

The effect of heart-and kidney-Benefiting Chinese Herbal Medicine on the function of cholinergic M-and Gamma amino-butyric acid (GABA) receptor in brain tissues of analogue dementia rats

Energy Technology Data Exchange (ETDEWEB)

Qizhong, Mo; Bin, Gong; Jun, Fang [Shanghai College of TCM, Shanghai (China); and others

1993-05-01

[sup 3]H-QNB and [sup 3]H-GABA were used as radioactive ligand for M-and GABA receptors respectively. M-and GABA receptors were assayed by radioligand binding assay (RBA) in cerebral cortex, hippocampus and cerebellum of analogue dementia rats. It was found that R[sub t] of M receptor was decreased in cerebral cortex and hippocampus and R[sub t] of GABA receptor was decreased in cerebellum of analogue dementia rats. The dissociation constant (K[sub D]) of M-receptor was decreased significantly in cerebral cortex and K[sub D] value of (GABA) receptor was decreased in cerebellum of analogue dementia rats. The decreased R[sub t] of M-and GABA receptor in brain tissue of analogue dementia rats was raised by Heart- and Kidney-Benefiting Chinese Herbs as well as hydergin.

Spin Label Studies of the Hemoglobin-Membrane Interaction During Sickle Hemoglobin Polymerization

International Nuclear Information System (INIS)

Falcon Dieguez, Jose E.; Rodi, Pablo; Lores Guevara, Manuel A.; Gennaro, Ana Maria

2009-12-01

An enhanced hemoglobin-membrane association has been previously documented in Sickle Cell Anemia. However, it is not known how this interaction is modified during the hemoglobin S polymerization process. In this work, we use a model of reconstituted erythrocytes from ghost membranes whose cytoskeleton proteins had been previously labeled with the 4-maleimido Tempo spin label, and that were subsequently resealed with hemoglobin S or A solutions. Using EPR spectroscopy, we studied the time dependence of the spectral W/S parameter, indicative of the conformational state of cytoskeleton proteins (mainly spectrin) under spontaneous deoxygenation, with the aim of detecting the eventual effects due to hemoglobin S polymerization. The differences observed in the temporal behaviour of W/S in erythrocytes reconstituted with both hemoglobins were considered as experimental evidence of an increment in hemoglobin S-membrane interaction, as a result of the polymerization process of hemoglobin S under spontaneous deoxygenation. (author)

Hemoglobin (image)

Science.gov (United States)

Hemoglobin is the most important component of red blood cells. It is composed of a protein called ... exchanged for carbon dioxide. Abnormalities of an individual's hemoglobin value can indicate defects in the normal balance ...

Earthquake Magnitude Relationships for the Saint Peter and Saint Paul Archipelago, Equatorial Atlantic

Science.gov (United States)

de Melo, Guilherme W. S.; do Nascimento, Aderson F.

2018-03-01

We have investigated several relationships between ML, M(NEIC) and Mw for the earthquakes locally recorded in the Saint Peter and Saint Paul Archipelago (SPSPA), Equatorial Atlantic. Because we only have one station in the area, we could not derive attenuation relations for events recorded at different distances at different stations. Our approach was then to compare our ML estimates with magnitudes reported by NEIC. This approach produced acceptable results particularly for epicentral distance smaller than 100 km. For distances greater that 100 km, there is a systematic increase in the residuals probable due to the lack of station correction and our inability to accurately estimate Q. We also investigate the Mw—M(NEIC) relationship. We find that Mw estimates using S-wave produce smaller residuals when compared with both M(NEIC). Finally, we also investigate the ML—Mw relationship and observe that given the data set we have, the 1:1 holds. We believe that the use of the present methodologies provide consistent magnitude estimates between all the magnitudes investigated that could be used to better assess seismic hazard in the region.

Hemoglobin

Science.gov (United States)

1993-03-08

affinity, which is less at low levels of hemoglobin saturation, increases markedly as fractional saturation increases. Thus, high affinity for 02 at... diphosphoglycerate (2,3-DPG), and carbon dioxide (Co 2). Since they are linked to 02 binding, they are called oxygen-linked effectors. The oxygen...hemoglobin molecule because of the negative charge of the ions. 2,3- Diphosphoglycerate is a molecule formed during the breakdown of sugar in normal human

the vision of divine light in saint gregory palamas's theology

African Journals Online (AJOL)

in Romania. 2. THE RECEPTION OF SAINT GREGORY PALAMAS'S. THEOLOGY IN THE WEST. In the West, the reception of Saint Gregory Palamas's theology, as it is known, was rather a negative one. His doctrine of divine light was rejected, because it postulated the possibility of seeing God with corporeal eyes. (Lossky ...

La collégiale Saint-Étienne de Neuvy-Saint-Sépulchre (Indre The collegiate church of Saint-Étienne at Neuvy-Saint-Sépulchre (Indre, France. A recent building survey of the rotunda and the nave

Directory of Open Access Journals (Sweden)

Simon Bryant

2006-05-01

Full Text Available La collégiale Saint-Étienne à Neuvy-Saint-Sépulchre (Indre est un des rares édifices subsistants en France qui soit conçu comme une copie de l’église du Saint-Sépulcre de Jérusalem. L’étude archéologique, réalisée pendant des travaux, a permis de préciser les étapes de son évolution depuis sa fondation dans les années 1040. La rotonde, avec ses onze piliers centraux, a été ajoutée à l’extrémité ouest d’une église antérieure. Le mur extérieur a été achevé dans cette première phase mais la cage centrale, qui abritait l’édicule, n’a été terminée qu’au début du XIIe s. Pendant la deuxième moitié du XIIe s., l’église est profondément remaniée et sans doute raccourcie avec la création d’un chevet plat. La voûte primitive est remplacée par trois travées de croisées d’ogives. L’édifice sert de refuge pendant la guerre de Cent Ans, ce qui provoque certains dégâts, partiellement réparés. Malgré les ruptures évidentes dans la construction et un plan irrégulier, l’étude montre que l’édifice est le résultat de la poursuite d’un projet architectural cohérent et original visant à évoquer l’église du Saint-Sépulcre de Jérusalem.The collegiate church of Saint-Étienne at Neuvy-Saint-Sépulchre (Department of the Indre, France is one of the few surviving rotundas built as a copy of the Holy-Sepulcre of Jerusalem during the mid 11th century. Many theories have been advanced concerning the history and the dating of this unusual monument. A recent building survey, carried out during restoration work, has managed to clarify the main phases of its evolution. The rotunda, with eleven central pillars placed around the reliquary monument, was added onto the west end of an existing church during the 1040’s. The two upper stories of the central cage, however, were not finished until the early 12th century. During the second half of the 12th century, the church was extensively

THE INFLUENCE OF MOTIVATING OPERATIONS ON GENERALIZATION PROBES OF SPECIFIC MANDS BY CHILDREN WITH AUTISM

OpenAIRE

Fragale, Christina L; O'Reilly, Mark F; Aguilar, Jeannie; Pierce, Nigel; Lang, Russell; Sigafoos, Jeff; Lancioni, Giulio

2012-01-01

We investigated the influence of motivating operations on the generalization of newly taught mands across settings and communication partners for 3 children with autism. Two conditions were implemented prior to generalization probes. In the first condition, participants were given access to a preferred item until they rejected the item (i.e., abolishing operation). In the second condition, the item was not available to participants prior to generalization probes (i.e., establishing operation)...

Conseqüências no crescimento de maxila e mandíbula de defeito ósseo cirúrgico no ramo da mandíbula de ratos Consequences on the growth of maxilla and mandible of surgical bone defect on mandibular ramus of rats

Directory of Open Access Journals (Sweden)

Lucimar Rodrigues

2004-10-01

Full Text Available OBJETIVO: Analisar as conseqüências no crescimento de maxila e mandíbula de defeito ósseo cirúrgico simulando fratura no ramo da mandíbula. MÉTODOS: Foram utilizados 25 ratos Wistar com um mês de idade. Sob anestesia geral e por meio de incisão submandibular. Foi realizada osteotomia vertical no ramo da mandíbula do lado direito com emprego de motor cirúrgico. Após período de dois meses os animais foram sacrificados, os tecidos moles retirados e as hemimandíbulas desarticuladas. Foram realizadas incidências radiográficas axiais para o crânio e laterais para as hemimandíbulas. A seguir, por intermédio de um sistema de computador foram obtidas medidas lineares da maxila e das hemimandíbulas. Foi empregado o teste "t" de Student para verificação da significância da diferença entre os lados experimental e controle. RESULTADOS: A diferença foi significante para a altura do ramo (p=0,010 e comprimento da mandíbula referente ao côndilo (p=0,015 e ao ângulo (pPURPOSE: To analyse the consequences on the growth of maxilla and mandible of a surgical bone defect that simulates a mandibular ramus fracture. METHODS: A group of 25 one-month-old Wistar rats were used in this study. Under general anesthesia, and through a submandibular incision, a vertical osteotomy on the right side of the mandibular ramus was made using a surgical drill. The animals were sacrificed after two months, soft tissues removed, and the mandible disarticulated. The skull was submitted to axial radiograph and the hemimandibles to a lateral radiograph. With these, cephalometric mensurations were made through a computer system, and the obtained values submitted to Student's t-test. RESULTS: The height of the mandibular ramus presented significant difference (p=0,010 as well as the length of mandible, both to the condyle (P=0,015 and the angle (p=0,001. There was no significant difference to the mensurations of the maxilla. CONCLUSION: The consequences of the

A New Pilgrimage in Portugal: Following the Steps of Saint Nuno

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Carla Braga

2013-12-01

Full Text Available Tourism is an ever-changing reality. Pilgrimages need different stimuli to attract new followers. In this context we propose to create an itinerary following the steps of Saint Nuno of Santa Maria, canonized in 2009. Nuno Álvares Pereira was the real name of Saint Nuno, who was granted the title Constable of Portugal, and became a historic legend in the independence wars of Portugal during the political crisis of 1383-1385. After fighting against the power of Castile (Spain he decided to devote his life to religion as a Carmelite Friar. He represents the perfect hero of the Middle Ages - associating military prowess with a religious character. Our proposal is to create a religious itinerary based on facts of Saint Nuno’s life, so that any visitor may easily discover the country following the steps of this saint who was deeply devoted to the Virgin - in fact, all temples he founded and restored were dedicated to Our Lady. One of his most famous foundations is the Carmelite Convent in Lisbon, which long after his death was an outstanding popular pilgrimage place, and today is still a strong visitor attraction. This exploratory study is based on literature review and text analyses about religious tourism, itinerary design and the life of the saint. The fieldwork includes acknowledging the different sites related to the saint. We believe that it is possible to create an original religious itinerary in Portugal, which will attract not only foreigners but also domestic visitors, increasing the ‘Production and Consumption of the Pilgrimage Tourism Experience’.

Acquisition of Picture Exchange-Based vs. Signed Mands and Implications to Teach Functional Communication Skills to Children with Autism

Science.gov (United States)

Nam, Sang S.; Hwang, Young S.

2016-01-01

A literature review was conducted to describe important concepts involved in functional analysis of verbal behavior as well as to evaluate empirical research findings on acquisition of picture exchange-based vs. signed mands to suggest instructional implications for teachers and therapists to teach functional communication skills to children with…

Radioimmunochemical characterization of hemoglobins Lepore and Kenya: unique antigenic determinants located on hybrid hemoglobins

International Nuclear Information System (INIS)

Garver, F.A.; Altay, G.; Baker, M.M.; Gravely, M.; Huisman, T.H.J.

1978-01-01

Antisera were produced in rabbits to the three known types of Lepore hemoglobins, which contain hybrid delta-β non-α-chains, and to hemoglobin Kenya, which has a hybrid γ-β non-α-chain. By using a sensitive radioimmunoassay technique, the absorbed antisera were shown to contain an antibody population that was specific for the hybrid hemoglobin and did not cross-react with normal hemoglobins. However, with the absorbed Lepore-specific antisera, the three known types of Lepore hemoglobins were antigenically indistinguishable from each other, suggesting that antibodies are not produced to the primary structural differences which define the three non-α-chains of the Lepore hemoglobins. These studies demonstrate that the non-α-subunits of hemoglobins Lepore and Kenya possess unique antigenic determinant sites, evidently resulting from an altered polypeptide conformation

Locality and Distance in Cults of Saints in Medieval Norway

DEFF Research Database (Denmark)

Petersen, Nils Holger

2017-01-01

A discussion of the Norwegian medieval cult of the purported Irish St Sunniva, a cult in which holiness is seen as foreign and distant in the cultural memory of the saint.......A discussion of the Norwegian medieval cult of the purported Irish St Sunniva, a cult in which holiness is seen as foreign and distant in the cultural memory of the saint....

Fish hemoglobins

Directory of Open Access Journals (Sweden)

P.C. de Souza

2007-06-01

Full Text Available Vertebrate hemoglobin, contained in erythrocytes, is a globular protein with a quaternary structure composed of 4 globin chains (2 alpha and 2 beta and a prosthetic group named heme bound to each one. Having myoglobin as an ancestor, hemoglobin acquired the capacity to respond to chemical stimuli that modulate its function according to tissue requirements for oxygen. Fish are generally submitted to spatial and temporal O2 variations and have developed anatomical, physiological and biochemical strategies to adapt to the changing environmental gas availability. Structurally, most fish hemoglobins are tetrameric; however, those from some species such as lamprey and hagfish dissociate, being monomeric when oxygenated and oligomeric when deoxygenated. Fish blood frequently possesses several hemoglobins; the primary origin of this finding lies in the polymorphism that occurs in the globin loci, an aspect that may occasionally confer advantages to its carriers or even be a harmless evolutionary remnant. On the other hand, the functional properties exhibit different behaviors, ranging from a total absence of responses to allosteric regulation to drastic ones, such as the Root effect.

Determinação do sexo e estimativa da idade por meio de mensurações em mandíbulas secas de adultos

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Victor Magalhães Santos Pinto

2017-09-01

Full Text Available A Odontologia Legal exerce um papel importante no processo de identificação de indivíduos, principalmente, quando é recebido para o processo de análise, o crânio da vítima. Este possui estruturas particulares que podem fornecer dados suficientes para a determinação do sexo de um indivíduo. Outra estrutura que também demonstra características seguras é a mandíbula. O objetivo desse trabalho foi determinar as características diferenciais entre partes de crânios pertencentes a indivíduos do sexo masculino e feminino, de diferentes idades, por meio da análise das distâncias entre os processos coronóides e as distâncias interbasal da língula em mandíbula seca. A pesquisa foi realizada no Laboratório de Anatomia na Universidade UNIME, utilizando-se 160 mandíbulas, sendo 81 do sexo feminino e 79 do sexo masculino, compreendidas entre a faixa etária de 21 a 95 anos. As amostras estudadas foram de pessoas indigentes cujas famílias não reclamaram os ossos no tempo hábil administrativo estabelecido pelo cemitério, iriam ser incinerados e pertencem atualmente ao Laboratório. De acordo com a análise estatística, houve um índice de acerto de 78,9% por regressão logística e 71,26% por análise discriminante. Houve também a verificação da média e do intervalo de confiança utilizando o teste t e a regressão linear múltipla para a predição da idade, evidenciando que o modelo não foi significativo (p = 0,4631. Após análises das mandíbulas secas, conseguiu-se validar as distâncias do processo coronóide aos sexos referidos.

Alpha chain hemoglobins with electrophoretic mobility similar to that of hemoglobin S in a newborn screening program.

Science.gov (United States)

Silva, Marcilene Rezende; Sendin, Shimene Mascarenhas; Araujo, Isabela Couto de Oliveira; Pimentel, Fernanda Silva; Viana, Marcos Borato

2013-01-01

To characterize alpha-chain variant hemoglobins with electric mobility similar to that of hemoglobin S in a newborn screening program. β(S) allele and alpha-thalassemia deletions were investigated in 14 children who had undefined hemoglobin at birth and an electrophoretic profile similar to that of hemoglobin S when they were six months old. Gene sequencing and restriction enzymes (DdeI, BsaJI, NlaIV, Bsu36I and TaqI) were used to identify hemoglobins. Clinical and hematological data were obtained from children who attended scheduled medical visits. THE FOLLOWING ALPHA CHAIN VARIANTS WERE FOUND: seven children with hemoglobin Hasharon [alpha2 47(CE5) Asp>His, HbA2:c.142G>C], all associated with alpha-thalassemia, five with hemoglobin Ottawa [alpha1 15(A13) Gly>Arg, HBA1:c.46G>C], one with hemoglobin St Luke's [alpha1 95(G2) Pro>Arg, HBA1:c.287C>G] and another one with hemoglobin Etobicoke [alpha212 84(F5) Ser>Arg, HBA212:c.255C>G]. Two associations with hemoglobin S were found: one with hemoglobin Ottawa and one with hemoglobin St Luke's. The mutation underlying hemoglobin Etobicoke was located in a hybrid α212 allele in one child. There was no evidence of clinically relevant hemoglobins detected in this study. Apparently these are the first cases of hemoglobin Ottawa, St Luke's, Etobicoke and the α212 gene described in Brazil. The hemoglobins detected in this study may lead to false diagnosis of sickle cell trait or sickle cell disease when only isoelectric focusing is used in neonatal screening. Additional tests are necessary for the correct identification of hemoglobin variants.

Detection of Sickle Cell Hemoglobin in Haiti by Genotyping and Hemoglobin Solubility Tests

Science.gov (United States)

Carter, Tamar E.; von Fricken, Michael; Romain, Jean R.; Memnon, Gladys; St. Victor, Yves; Schick, Laura; Okech, Bernard A.; Mulligan, Connie J.

2014-01-01

Sickle cell disease is a growing global health concern because infants born with the disorder in developing countries are now surviving longer with little access to diagnostic and management options. In Haiti, the current state of sickle cell disease/trait in the population is unclear. To inform future screening efforts in Haiti, we assayed sickle hemoglobin mutations using traditional hemoglobin solubility tests (HST) and add-on techniques, which incorporated spectrophotometry and insoluble hemoglobin separation. We also generated genotype data as a metric for HST performance. We found 19 of 202 individuals screened with HST were positive for sickle hemoglobin, five of whom did not carry the HbS allele. We show that spectrophotometry and insoluble hemoglobin separation add-on techniques could resolve false positives associated with the traditional HST approach, with some limitations. We also discuss the incorporation of insoluble hemoglobin separation observation with HST in suboptimal screening settings like Haiti. PMID:24957539

L’impasse des verbeuses ou la rivière enfouie

OpenAIRE

Baptiste-Marrey,

2017-01-01

Cela fait vingt ans que j’habite Gentilly, un demi-siècle que je crapahute dans le Val-de-Marne (on dit aussi le 94), bastion historique du communisme français, dernier conseil général aux mains de ce qui survit du P.C.F. Sur l’autre rive de la Seine, c’est le 94 bourgeois (Nogent, Saint-Maur, Saint-Mandé, Vincennes) et de l’autre côté de ce qui fut la Nationale 10, ce sont les Hauts-de-Seine sarkozystes (le 92). Villejuif est le siège de la « communauté d’agglomération » (sans s) du Val-de-B...

Alpha chain hemoglobins with electrophoretic mobility similar to that of hemoglobin S in a newborn screening program

Directory of Open Access Journals (Sweden)

Marcilene Rezende Silva

2013-01-01

Full Text Available OBJECTIVE: To characterize alpha-chain variant hemoglobins with electric mobility similar to that of hemoglobin S in a newborn screening program. METHODS: βS allele and alpha-thalassemia deletions were investigated in 14 children who had undefined hemoglobin at birth and an electrophoretic profile similar to that of hemoglobin S when they were six months old. Gene sequencing and restriction enzymes (DdeI, BsaJI, NlaIV, Bsu36I and TaqI were used to identify hemoglobins. Clinical and hematological data were obtained from children who attended scheduled medical visits. RESULTS: The following alpha chain variants were found: seven children with hemoglobin Hasharon [alpha2 47(CE5 Asp>His, HbA2:c.142G>C], all associated with alpha-thalassemia, five with hemoglobin Ottawa [alpha1 15(A13 Gly>Arg, HBA1:c.46G>C], one with hemoglobin St Luke's [alpha1 95(G2 Pro>Arg, HBA1:c.287C>G] and another one with hemoglobin Etobicoke [alpha212 84(F5 Ser>Arg, HBA212:c.255C>G]. Two associations with hemoglobin S were found: one with hemoglobin Ottawa and one with hemoglobin St Luke's. The mutation underlying hemoglobin Etobicoke was located in a hybrid α212 allele in one child. There was no evidence of clinically relevant hemoglobins detected in this study. CONCLUSION: Apparently these are the first cases of hemoglobin Ottawa, St Luke's, Etobicoke and the α212 gene described in Brazil. The hemoglobins detected in this study may lead to false diagnosis of sickle cell trait or sickle cell disease when only isoelectric focusing is used in neonatal screening. Additional tests are necessary for the correct identification of hemoglobin variants.

The harmonious relationship between faith and science from the perspective of some great saints: A brief comment.

Science.gov (United States)

Cortés, Manuel E; Del Río, Juan Pablo; Vigil, Pilar

2015-02-01

The objective of this editorial is to show that a harmonious relationship between science and faith is possible, as exemplified by great saints of the Catholic Church. It begins with the definitions of science and faith, followed by an explanation of the apparent conflict between them. A few saints that constitute an example that a fruitful relationship between these two seemingly opposed realities has been possible are Saint Albert the Great, Saint John of the Cross, Saint Giuseppe Moscati, and Saint Edith Stein, among others, and this editorial highlights their deep contributions to the dialogue between faith and reason. This editorial ends with a brief discussion on whether it is possible to be both a scientist and a man of faith.

Nuclear safety and radiation protection report of the Saint-Alban-Saint-Maurice nuclear facilities - 2011

International Nuclear Information System (INIS)

2012-01-01

This safety report was established under the article 21 of the French law no. 2006-686 of June 13, 2006 relative to nuclear safety and information transparency. It presents, first, the facilities of the Saint-Alban-Saint-Maurice nuclear power plant (INB 119 and 120, Isere (FR)). Then, the nuclear safety and radiation protection measures taken regarding the facilities are reviewed: nuclear safety definition, radiation protection of intervening parties, safety and radiation protection improvement paths, crisis management, external and internal controls, technical situation of facilities, administrative procedures in progress. The incidents and accidents which occurred in 2011, are reported as well as the radioactive and non-radioactive (chemical, thermal) effluents discharge in the environment. Finally, The radioactive materials and wastes generated by the facilities are presented and sorted by type of waste, quantities and type of conditioning. Other environmental impacts (noise) are presented with their mitigation measures. Actions in favour of transparency and public information are presented as well. The document concludes with a glossary and a list of recommendations from the Committees for health, safety and working conditions. (J.S.)

Nuclear safety and radiation protection report of the Saint-Alban-Saint-Maurice nuclear facilities - 2010

International Nuclear Information System (INIS)

2011-06-01

This safety report was established under the article 21 of the French law no. 2006-686 of June 13, 2006 relative to nuclear safety and information transparency. It presents, first, the facilities of the Saint-Alban-Saint-Maurice nuclear power plant (INB 119 and 120, Isere (FR)). Then, the nuclear safety and radiation protection measures taken regarding the facilities are reviewed: nuclear safety definition, radiation protection of intervening parties, safety and radiation protection improvement paths, crisis management, external and internal controls, technical situation of facilities, administrative procedures in progress. The incidents and accidents which occurred in 2010, are reported as well as the radioactive and non-radioactive (chemical, thermal) effluents discharge in the environment. Finally, The radioactive materials and wastes generated by the facilities are presented and sorted by type of waste, quantities and type of conditioning. Other environmental impacts (noise) are presented with their mitigation measures. Actions in favour of transparency and public information are presented as well. The document concludes with a glossary and a list of recommendations from the Committees for health, safety and working conditions. (J.S.)

Hemoglobin Wayne Trait with Incidental Polycythemia.

Science.gov (United States)

Ambelil, Manju; Nguyen, Nghia; Dasgupta, Amitava; Risin, Semyon; Wahed, Amer

2017-01-01

Hemoglobinopathies, caused by mutations in the globin genes, are one of the most common inherited disorders. Many of the hemoglobin variants can be identified by hemoglobin analysis using conventional electrophoresis and high performance liquid chromatography; however hemoglobin DNA analysis may be necessary in other cases for confirmation. Here, we report a case of a rare alpha chain hemoglobin variant, hemoglobin Wayne, in a 47-year-old man who presented with secondary polycythemia. Capillary zone electrophoresis and high performance liquid chromatography revealed a significant amount of a hemoglobin variant, which was further confirmed by hemoglobin DNA sequencing as hemoglobin Wayne. Since the patient was not homozygous for hemoglobin Wayne, which is associated with secondary polycythemia, the laboratory diagnosis in this case was critical in ruling out hemoglobinopathy as the etiology of his polycythemia. © 2017 by the Association of Clinical Scientists, Inc.

Particular intervention plan of the Saint Alban-Saint Maurice l'Exil Nuclear Power Plant

International Nuclear Information System (INIS)

2010-01-01

The Particular intervention plan (PPI in French) is an emergency plan which foresees the measures and means to be implemented to address the potential risks of the presence and operation of a nuclear facility. This plan is implemented and developed by the Prefect in case of nuclear accident (or incident leading to a potential accident), the impact of which extending beyond the facility perimeter. It represents a special section of the organisation plan for civil protection response (ORSEC plan). The PPI foresees the necessary measures and means for crisis management during the first hours following the accident and is triggered by the Department Prefect according to the information provided by the facility operator. Its aim is to protect the populations leaving within 10 km of the facility against a potential radiological hazard. The PPI describes: the facility, the intervention area, the protection measures for the population, the conditions of emergency plan triggering, the crisis organisation, the action forms of the different services, and the post-accident stage. This document is the public version of the Particular intervention plan of the Saint Alban-Saint Maurice NPP (Isere, France)

The Hemoglobin E Thalassemias

Science.gov (United States)

Fucharoen, Suthat; Weatherall, David J.

2012-01-01

Hemoglobin E (HbE) is an extremely common structural hemoglobin variant that occurs at high frequencies throughout many Asian countries. It is a β-hemoglobin variant, which is produced at a slightly reduced rate and hence has the phenotype of a mild form of β thalassemia. Its interactions with different forms of α thalassemia result in a wide variety of clinical disorders, whereas its coinheritance with β thalassemia, a condition called hemoglobin E β thalassemia, is by far the most common severe form of β thalassemia in Asia and, globally, comprises approximately 50% of the clinically severe β-thalassemia disorders. PMID:22908199

Of volcanoes, saints, trash, and frogs

DEFF Research Database (Denmark)

Andersen, Astrid Oberborbeck

, at the same time as political elections and economic hardship. During one year of ethnographic fieldwork volcanoes, saints, trash and frogs were among the nonhuman entities referred to in conversations and engaged with when responding to the changes that trouble the world and everyday life of Arequipans...

Phylogeny of Echinoderm Hemoglobins.

Directory of Open Access Journals (Sweden)

Ana B Christensen

Full Text Available Recent genomic information has revealed that neuroglobin and cytoglobin are the two principal lineages of vertebrate hemoglobins, with the latter encompassing the familiar myoglobin and α-globin/β-globin tetramer hemoglobin, and several minor groups. In contrast, very little is known about hemoglobins in echinoderms, a phylum of exclusively marine organisms closely related to vertebrates, beyond the presence of coelomic hemoglobins in sea cucumbers and brittle stars. We identified about 50 hemoglobins in sea urchin, starfish and sea cucumber genomes and transcriptomes, and used Bayesian inference to carry out a molecular phylogenetic analysis of their relationship to vertebrate sequences, specifically, to assess the hypothesis that the neuroglobin and cytoglobin lineages are also present in echinoderms.The genome of the sea urchin Strongylocentrotus purpuratus encodes several hemoglobins, including a unique chimeric 14-domain globin, 2 androglobin isoforms and a unique single androglobin domain protein. Other strongylocentrotid genomes appear to have similar repertoires of globin genes. We carried out molecular phylogenetic analyses of 52 hemoglobins identified in sea urchin, brittle star and sea cucumber genomes and transcriptomes, using different multiple sequence alignment methods coupled with Bayesian and maximum likelihood approaches. The results demonstrate that there are two major globin lineages in echinoderms, which are related to the vertebrate neuroglobin and cytoglobin lineages. Furthermore, the brittle star and sea cucumber coelomic hemoglobins appear to have evolved independently from the cytoglobin lineage, similar to the evolution of erythroid oxygen binding globins in cyclostomes and vertebrates.The presence of echinoderm globins related to the vertebrate neuroglobin and cytoglobin lineages suggests that the split between neuroglobins and cytoglobins occurred in the deuterostome ancestor shared by echinoderms and vertebrates.

Changes in the Population Distribution and Transport Network of Saint Petersburg

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Xiaoling Li

2016-12-01

Full Text Available The authors explores the interdependence between demographic changes and transport network centrality, using Saint Petersburg as an example. The article describes the demographic data for the period 2002—2015 and the transportation network data of 2006. The authors employ several methods of demographic research; they identified the centre of gravity of the population, produce the standard deviational ellipsis and use the kernel density estimation. The street network centrality of Saint Petersburg was analyzed using the Multiple Centrality Assessment Model (MCA and the Urban Network Analysis Tool for ArcGIS. The analysis of the population distribution in Saint Petersburg shows that each area of the city has seen their population grow over the last thirteen years. However, it is the population of suburban areas that increased the most. The core area of the city has the tendency of outward diffusion, and the population gravity centre has been moving northwards. Spatial characteristics of the population growth, changes in the population gravity centre, the standard deviational ellipse and characteristics of the street network centrality show that Saint Petersburg is at the final stage of urbanization and its development pattern is similar to that of other major cities.

Gravimetric structure for the abyssal mantle massif of Saint Peter and Saint Paul peridotite ridge, Equatorial Atlantic Ocean, and its relation to active uplift

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KENJI F. MOTOKI

2014-06-01

Full Text Available This paper presents gravimetric and morphologic analyses based on the satellite-derived data set of EGM2008 and TOPEX for the area of the oceanic mantle massif of the Saint Peter and Saint Paul peridotite ridge, Equatorial Atlantic Ocean. The free-air anomaly indicates that the present plate boundary is not situated along the longitudinal graben which cuts peridotite ridge, but about 20 km to the north of it. The high Bouguer anomaly of the peridotite ridge suggests that it is constituted mainly by unserpentinised ultramafic rocks. The absence of isostatic compensation and low-degree serpentinisation of the ultramafic rocks indicate that the peridotite ridge is sustained mainly by active tectonic uplift. The unparallel relation between the transform fault and the relative plate motion generates near north-south compression and the consequent tectonic uplift. In this sense, the peridotite massif is a pressure ridge due to the strike-slip displacement of the Saint Paul Transform Fault.

Saint Lucia revisited.

Science.gov (United States)

Bos, R; Février, M; Knudsen, A B

1988-10-01

The eastern Caribbean island of Saint Lucia is now famous in parasitological history as the setting for a major programme of schistosomiasis control'. Perhaps less well-known are the island's effective control of many intestinal parasites, and elimination of malaria, such that the current patterns of mortality and other demographic indicators now resemble those of industrialized countries. More recently, the island has become the focus for another community-based health programme as the Caribbean region again comes to grips with Aedes aegypti and its recently imported relative, Aedes albopictus, important vectors of yellow fever and dengue viruses (see Box 1).

Please exercise extreme caution at the Saint-Genis roundabout

CERN Multimedia

2015-01-01

In the interests of enhanced safety, a new pathway for pedestrians and cyclists has been constructed around the outside of the Saint-Genis roundabout. However, the markings of the previous cycle path, which is now closed to traffic, are still visible and can cause confusion. We therefore call on everyone to exercise extreme caution and to use the new pathway.   New two-way markings have been laid out, inviting pedestrians and cyclists coming from the direction of Saint-Genis-Pouilly to go towards the Swiss border or CERN Entrance E using the left-hand side of the roundabout (i.e. the Prévessin side). So, from now on, cyclists must no longer go around the roundabout on the right-hand side and pedestrians will no longer have to cross the D884 dual carriageway. Similarly, people staying at the Saint-Genis hostel are invited to follow these new markings to get to CERN or to return to the hostel, which means they will avoid having to cross the D35 highway at a spot where traffic is...

The effects of parent-implemented PECS training on improvisation of mands by children with autism.

Science.gov (United States)

Ben Chaabane, Delia B; Alber-Morgan, Sheila R; DeBar, Ruth M

2009-01-01

The present study examined the extent to which mothers were able to train their children, 2 boys with autism, to exchange novel pictures to request items using the picture exchange communication system (PECS). Generalization probes assessing each child's ability to mand for untrained items were conducted throughout conditions. Using a multiple baseline design, results demonstrated that both children improvised by using alternative symbols when the corresponding symbol was unavailable across all symbol categories (colors, shapes, and functions) and that parents can teach their children to use novel pictorial response forms.

Sanctified madness: the God-intoxicated saints of Bengal.

Science.gov (United States)

Morinis, A

1985-01-01

The saintly madman is a familiar character in South Asia. To outer appearances he is no different from a lunatic, but the mad saint comes to be revered because his idiocy is popularly believed to arise from a different cause than ordinary madness. The common psychopath neglects social conventions because his consciousness is dimmed by incapacity; the saintly madman also breaches convention, but does so because his heightened consciousness has liberated him from the bonds of convention that entrap ordinary people. In the terms of Hinduism, he has tasted the divine nectar of God-realization and has returned to the human realm intoxicated by the experience. In this paper two popular God intoxicated saints of Bengal are discussed. The question is posed whether 'God intoxication' can be considered a culture-bound syndrome of Bengal. The concept of 'culture bound syndrome' is found to be too narrow to encompass the most significant issues to arise from reflection on the characteristics of the God intoxicated. These larger issues have to do with the relationship between cultural practices and models and mental states (whether deviant, as implied by the term 'syndrome' although deviance does not always carry the negative connotation implicit in 'syndrome', or normal). It is suggested that all cultures culture a limited range of mental states and thus the questions posed by the notion of culture bound syndromes are subsumed by larger questions about the relationship of all mind-states to the socio-cultural environment which conditions them. The conclusion is that God intoxication is indeed a uniquely Bengali mental condition, with variants throughout South Asia and kinship to other mystical states, but that the concept of 'syndrome' is not useful.

Fish hemoglobins

OpenAIRE

Souza,P.C. de; Bonilla-Rodriguez,G.O.

2007-01-01

Vertebrate hemoglobin, contained in erythrocytes, is a globular protein with a quaternary structure composed of 4 globin chains (2 alpha and 2 beta) and a prosthetic group named heme bound to each one. Having myoglobin as an ancestor, hemoglobin acquired the capacity to respond to chemical stimuli that modulate its function according to tissue requirements for oxygen. Fish are generally submitted to spatial and temporal O2 variations and have developed anatomical, physiological and biochemica...

President Ilves kõneles Saint Gallenis

Index Scriptorium Estoniae

2009-01-01

President Toomas Hendrik Ilves esines 9. mail 2009 Šveitsis, Saint Galleni 39. majandus- ja poliitikasümpoosionil kõnega teemal "20 aastat pärast Berliini müüri langemist - Euroopa uued väljakutsed". Vabariigi President töövisiidil Šveitsis 08.-09.05.2009

Manipulation of Motivating Operations and Use of a Script-Fading Procedure to Teach Mands for Location to Children with Language Delays

Science.gov (United States)

Howlett, Melissa A.; Sidener, Tina M.; Progar, Patrick R.; Sidener, David W.

2011-01-01

The effects of contriving motivating operations (MOs) and script fading on the acquisition of the mand "Where's [object]?" were evaluated in 2 boys with language delays. During each session, trials were alternated in which high-preference items were present (abolishing operation [AO] trials) or missing (establishing operation [EO] trials) from…

SUFI SAINTS IN KARNATAKA

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Maruti KAMBLE

2011-01-01

Full Text Available Karnataka is one of the most important southern states of India. It has gainedprominence politically, socio-economically, philosophically and in many otherways. It is a meeting place of many religious philosophies and a melting pointhelping to achieve the synthesis of the teaching ofmany religious reforms. Sufismis a mystic religion. It is an offshoot of Islam. Sufi saints are the followers ofALLAH and the practice of peace, benevolence and tolerance. They promulgateda religion based on the concept of love, the love with the fellow being and thelove with the ultimate or the creator. Sufi saintsand Sufism are an existing realityin Indian socio-religious fold and it has contributed for a healthy and amicablesocial order. It has lead to a new social set-up full of values, and discipline.Sufism as a religion of the teaming millions becamethe practicing system amongthe people of India as well as Karnataka. This study of Sufi Saints in Karnataka isthus an inspired attempt to portray this religion of the soul and heart. Muchemphasis is laid on the concepts and special aspects of Sufism, along with variouspractices found in it. I was always attracted by its spiritual significance andpractical essence and also its profound influence on the general public. Hence, mylittle attempt to explain the same through the lifestories of some of the great Sufisaints in Karnataka.

Hemoglobin C, S-C, and E Diseases

Science.gov (United States)

... quickly than others, resulting in chronic anemia. Hemoglobin C disease Hemoglobin C disease occurs mostly in blacks. ... a common complication of hemoglobin C disease. Hemoglobin S-C disease Hemoglobin S-C disease occurs in people who ...

Charge resolution of a Hungarian brand CR-39(MA-ND) detector exposed to a 84Kr beam of energy 0.45A GeV

International Nuclear Information System (INIS)

Bhattacharyya, D.P.; Basu, B.; Pal, P.; Mukherjee, S.C.; Ganguly, A.K.; Hunyady, I.

1990-01-01

The Hungarian brand CR-39(MA-ND) plastic has been irradiated with a 84 Kr ion beam of energy 0.45A GeV and etched for four different etching times, viz. 4, 6, 8 and 12 h. The estimated charge resolution of a CR-39(MA-ND) detector for registering the nuclei 32 ≤ Z ≤ 36 was found to be 0.18e which is close to our previous observation of the response with a CR-39(DOP) Pershore made plate exposed to a 1.88A GeV 56 Fe beam at the Lawrence Berkeley Laboratory's Bevalac. It was found that the estimated etch rate ratio V T /V G is independent of etching time. The cone length and minor axis of the etch pits has been found to increase with etching time. (orig.)

Des saints pour la fiction, des saints face à l’Histoire

Directory of Open Access Journals (Sweden)

Aude Bonord

2008-12-01

Full Text Available Pourquoi des écrivains non confessionnels (Joseph Delteil, Blaise Cendrars, Christian Bobin, Sylvie Germain, Claude Louis-Combet ont-ils choisi de réécrire des vies de saints au XXe siècle ? Le parti pris est surprenant à plusieurs titres. L’hagiographie est un genre historique et religieux, terrain a priori réservé aux historiens, aux ecclésiastiques ou aux écrivains catholiques. De plus, à la fin du siècle, l’hagiographie est sortie du champ littéraire avec la disparition du groupe des écrivains catholiques. Curieusement, nos auteurs vont s’inscrire dans ces décalages non seulement pour définir leur place par rapport à l’hagiographe traditionnel mais aussi pour s’approprier le genre. Amorces fictionnelles et objets de rêveries poétiques, les vies de saints sont soumises à la fantaisie littéraire de ces auteurs au statut improbable. Dans un dialogue avec les sources allant du jeu à la contestation, les écrivains utilisent l’intertexte hagiographique, et notamment son système narratif, pour s’évader dans le merveilleux mais encore pour penser leur époque. En effet, si éloignée de la réalité contemporaine soit-elle en apparence, l’hagiographie joue à la fois un rôle de contrepoint et de reflet du présent. La confrontation avec ces récits surgis du passé permet alors aux auteurs de repenser la marche de l’Histoire et l’inscription de l’Homme dans le temps.

Colourful sparkles of imaginary vistas: Saintly beauty in the eyes of the beholder

Directory of Open Access Journals (Sweden)

Dimitrova Elizabeta

2013-01-01

Full Text Available The article deals with the issue of physical appearance of the saintly images in Byzantine painting and the manners of its conception. In that regard, the paper offers categorization of the portraiture in which different saints receive distinctive aesthetic code appropriate to their type of “attractiveness”.

Interaction of thyroid hormone and hemoglobin: nature of the interaction and effect of hemoglobin on thyroid hormone radioimmunoassay

International Nuclear Information System (INIS)

Davis, P.J.; Yoshida, K.; Schoenl, M.

1980-01-01

Gel filtration of human erythrocyte (RBC) lysate incubated with labeled thyroxine (Tu) or triiodothyronine (Tt) revealed co-elution of a major iodothyronine-binding fraction (R-2) and hemoglobin. Solutions of purified human hemoglobin and Tt also showed co-elution of hormone and hemoglobin. Because hematin and protoporphyrin were shown to bind labeled Tt, the oxygen-binding site on hemoglobin was excluded as the site of iodothyronine-hemoglobin interaction. Analysis of hormone binding by heme and globin moieties showed Tt binding to be limited to the heme fraction. Addition of excess unlabeled Tt to hemoglobin or heme incubated with labeled Tt indicated 75% to 90% of hormone binding was poorly dissociable. These observations suggested that the presence of hemoglobin in RBC lysate or in serum could influence the measurement of Tu and Tt by specific radioimmunoassay (RIA). Subsequent studies of the addition to serum of human hemoglobin revealed a significant reduction in Tt and Tu detectable by RIA in the presence of this protein. The effect was influenced by the concentration of hemoglobin and by duration and temperature of incubations of hemoglobin and serum prior to RIA

Rare copy of "Life of Saint Volo-dymyr" (Kyiv, 1670 from the manuscript Corpus of Lives of the Saints as a source for the 17th century Kyiv hagiography study

Directory of Open Access Journals (Sweden)

Bondar N. P.

2017-01-01

Full Text Available In the article a rare copy of an old-printed edition of “Life of Saint Volodymyr”, published circa 1670 in the Kyiv-Pechersk Lavra printing-house is characterized. The copy was included to Corpus of Lives of the Saints of the17th-18th centuries from the collection of Church and Archeological museum of Kyiv Ecclesiastical Academy (Fond 301, Unit 416L. According to the modern advances of codicology, archeology, filigranology it became possible to critically review the data of descriptions, which brought new results. The approximate dating of the old-printed edition, which has no title and imprint, is confirmed. The publishing history of “Life of Saint Volodymyr” is analyzed. The research of the copy is accompanied by the complex description of overall manuscript hagiography codex, the analysis of its content, specifics, ownership notes and paper filigrees. Archeographic analysis of Corpus of Lives of the Saints was combined with study of formation of Ukrainian hagiography complex of the second half of the 17th - the early 18th centuries.

Ácido mandélico: Modelado de su estructura y espectro de rotación

OpenAIRE

Lirio Martínez, Ángel

2016-01-01

En este trabajo, se ha realizado el modelado teórico del panorama conformacional de la molécula del ácido mandélico, mediante el uso de diversos tipos de métodos computacionales. Una vez obtenidos los confórmeros más estables, todos con energías menores a 1000 cm-1, se llevado a cabo un análisis de las interacciones intramoleculares que se dan. A partir de los datos obtenidos se ha realizado el modelado del espectro de rotación de cada confórmero, en el rango del espectro de 1...

Evaluation of OPPS model for plant operator's task simulation with Micro-SAINT

International Nuclear Information System (INIS)

Yoshida, Kazuo

1991-03-01

The development of a computer simulation method for cognitive behavior of operators under emergency conditions in nuclear power plant are being conducted at Japan Atomic Energy Research Institute (JAERI). As one of activities in this project, the task network modeling and simulation method has been evaluated with reproduced OPPS model using Micro-SAINT which is a PC software for task network analysis. OPPS is an operator's task simulation model developed by Oak Ridge National Laboratory. Operator's tasks under the condition of failure open of a safety relief valve in a BWR power plant has been analyzed as a sample problem with Micro-SAINT version of OPPS for the evaluation of task network analysis method. Furthermore, the fundamental capabilities of Micro-SAINT has been evaluated, and the task network in OPPS model has been also examined. As the results of this study, it has been clarified that random seed numbers in Micro-SAINT affect the probabilistic branching ratio and the distribution of task execution time calculated by Monte Carlo simulations, and the expression of network for a repeated task in the OPPS model leads to incorrect standard deviation in the case that a task execution time has some distribution. (author)

Síntese e caracterização de um novo composto obtido pela reação entre hidreto de trifenilestanho e ácido (±)-mandélico e avaliação de seu potencial biocida sobre o fungo Fusarium oxysporum f. sp. cubense

OpenAIRE

Barbiéri, Roberto Santos; Lima, Elysio Prado de; Silva, Samuel Ferreira da; Dias, Allan Kardec Carlos; Cardoso, Maria das Graças; Terra, Vilma Reis

2006-01-01

O presente artigo refere-se à síntese e caracterização de um novo composto organoestânico, pela reação de ácido (±)-mandélico e hidreto de trifenilestanho, em meio de acetonitrila e sob refluxo, [(C6H5)2SnMand 2] {Mand = C6H5CH(OH)COO], identificado por análise elementar de carbono e hidrogênio, espectroscopia no infravermelho e espectrometria de massa de alta resolução, para o qual foi proposta estrutura octaédrica com o grupo fenila em posição trans. Verificou-se que o composto apresenta aç...

Hemoglobin Test: MedlinePlus Lab Test Information

Science.gov (United States)

... page: https://medlineplus.gov/labtests/hemoglobintest.html Hemoglobin Test To use the sharing features on this page, please enable JavaScript. What is a Hemoglobin Test? A hemoglobin test measures the levels of hemoglobin ...

Rahmaninov ja Saint-Saens Estonia kontserdisaalis / Tiina Õun

Index Scriptorium Estoniae

Õun, Tiina, 1954-

2005-01-01

18. novembril Estonia kontserdisaalis toimuvast Sergei Rahmaninovi Klaverikontsert nr 2 ja "Kellad" ettekandest ja 29. novembril Toimuvast Camille Saint-Saensi ooperi "Simson ja Delila" kontsertettekandest

A new hemoglobin gene from soybean: a role for hemoglobin in all plants

DEFF Research Database (Denmark)

Anderson, C R; Jensen, E O; LLewellyn, D J

1996-01-01

We have isolated a new hemoglobin gene from soybean. It is expressed in cotyledons, stems of seedlings, roots, young leaves, and in some cells in the nodules that are associated with the nitrogen-fixing Bradyrhizobium symbiont. This contrasts with the expression of the leghemoglobins, which...... are active only in the infected cells of the nodules. The deduced protein sequence of the new gene shows only 58% similarity to one of the soybean leghemoglobins, but 85-87% similarity to hemoglobins from the nonlegumes Parasponia, Casuarina, and barley. The pattern of expression and the gene sequence...... indicate that this new gene is a nonsymbiotic legume hemoglobin. The finding of this gene in legumes and similar genes in other species strengthens our previous suggestion that genomes of all plants contain hemoglobin genes. The specialized leghemoglobin gene family may have arisen from a preexisting...

Saint-Augustin, Proudhon, antithèses

OpenAIRE

Pasteur , Sébastien

2005-01-01

Proudhon cite peu Saint-Augustin, mais le parallèle d'un contexte historique à son tournant offre matière à réfléchir. D'autant que Proudhon, anticlérical concède au Père de l'Eglise, un rang de philosophe, occasion d'aborder la fondation du discours philosophique au travers de la confession.

Les couleurs de Moscou et de Saint-Pétersbourg The colours of Moscow and Saint Petersburg

Directory of Open Access Journals (Sweden)

Andréi Éfimov

2008-05-01

Full Text Available Moscou et Saint-Pétersbourg ont connu une évolution chromatique similaire mais les différences en matière de plan, d’urbanisme et d’influences culturelles, et leurs rôles alternés de capitale ont déterminé leurs particularités chromatiques propres. Le Moscou en bois du xiiie siècle s’est enrichi d’un ensemble de cathédrales blanches aux coupoles dorées entourées par la muraille en brique rouge du Kremlin. Le style baroque a introduit dans l’architecture de Moscou et de Saint-Pétersbourg les couleurs bleu foncé, orange et verte. À l’époque du classicisme, on utilisait la palette officielle de teintes pastel à dominante ocre. L’achromatisme devient ensuite un trait distinctif de l’éclectisme en Russie. Au début du XXe siècle, l’Art nouveau ranime les villes grâce aux couleurs verte, orange et violette. Dans les années 1920 et 1930, les constructivistes utilisent les tons gris mais tentent d’élaborer un code chromatique homogène. L’architecture soviétique, quant à elle, reprend l’esthétique du classicisme « ocre ». La construction de masse des années 1960 aux années 1980 méconnaît la couleur, mais à la charnière des XXe et XXIe siècles apparaissent des approches professionnelles qui visent à créer un milieu chromatique similaire pour les deux villes.Moscow and Saint Petersburg experienced similar evolutions in colour, but the differences in their plans, urbanity and cultural influences, and their alternating roles as capitals, determined the particularities of their palettes. The wooden Moscow of the thirteenth century was enriched by a group of white cathedrals with golden domes, surrounded by the red brick wall of the Kremlin. Baroque, as an architectural style, introduced Moscow and Saint Petersburg to the colours dark blue, orange, and green. The official palette of classicism was pastel, predominantly ochre, while the period of eclecticism in Russia was achromatic. At the

Non-invasive hemoglobin monitoring.

Science.gov (United States)

Joseph, Bellal; Haider, Ansab; Rhee, Peter

2016-09-01

Technology has transformed the practice of medicine and surgery in particular over the last several decades. This change in practice has allowed diagnostic and therapeutic tests to be performed less invasively. Hemoglobin monitoring remains one of the most commonly performed diagnostic tests in the United States. Recently, non-invasive hemoglobin monitoring technology has gained popularity. The aim of this article is to review the principles of how this technology works, pros and cons, and the implications of non-invasive hemoglobin technology particularly in trauma surgery. Copyright © 2015 IJS Publishing Group Ltd. Published by Elsevier Ltd. All rights reserved.

Bathymetry of Lake Erie and Lake Saint Clair

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Bathymetry of Lake Erie and Lake Saint Clair has been compiled as a component of a NOAA project to rescue Great Lakes lake floor geological and geophysical data and...

Microseismic noise in the Saint Peter and Saint Paul Archipelago, equatorial Atlantic

Science.gov (United States)

de Queiroz, Daniel É.; do Nascimento, Aderson F.; Schimmel, Martin

2017-12-01

Microseismic noise, also known as ambient seismic noise, are continuous vibrations mostly composed of Rayleigh waves pervasively recorded in the mili Hertz to 1 Hz frequency range. Their precise source mechanisms are under investigations and related to atmospheric perturbations and ocean gravity waves. Our purpose is to show the behavior of the microseismic noise recorded in the Saint Peter and Saint Paul Archipelago (SPSPA) with respect to wind intensity and ocean waves height in this region, between the North and South Atlantic Ocean. We have recorded both primary microseisms (PM) 0.04-0.12 Hz and the secondary microseisms (SM) 0.12-0.4 Hz during almost four years (2012-2015) and we used frequency, temporal, spatial and statistical correlation analysis to do qualitative and quantitative analysis with respect to wind speed intensity and significant wave height for the same periods. The results indicate a good correlation between the PM and the SM noise in the region particularly during the winter in the Northern Hemisphere and a poor correlation during the summer. We have also shown that probably most of the PM are generated in the SPSPA itself. We note that the intensity of SM recorded in SPSPA appears to have a seasonal behavior with the summer and winter in the Northern Hemisphere, and seems to influence the correlation between the PM and the SM, suggesting that the sources of the PM and the SM are not related to the same atmospheric event and from different places. PM generation would occur near the SPSPA whilst the SM would have distant sources towards the North Atlantic.

Blood Test: Hemoglobin A1C

Science.gov (United States)

... Why Are Hemoglobin A1c Tests Done? When a child has diabetes, hemoglobin A1c levels are followed to see how well medicines are working. If a child with diabetes has a high hemoglobin A1c level, it may ...

Marketing analysis of brand Yves Saint Laurent in portfolio L´Oréal

OpenAIRE

Petránková, Tereza

2011-01-01

The aim of this thesis Marketing analysis of brand Yves Saint Laurent in portfolio L'Oréal is analysis of brand Yves Saint Laurent. The theoretical part focuses mainly on marketing, brand, luxury, analysis of brand and marketing strategies. Practical part based on the findings in theoretical part analysed brand - history, indentity, value and marketing strategy and reccomend new marketing strategies.

Hemoglobin estimation by the HemoCue® portable hemoglobin photometer in a resource poor setting

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Idriss Ali

2011-04-01

Full Text Available Abstract Background In resource poor settings where automated hematology analyzers are not available, the Cyanmethemoglobin method is often used. This method though cheaper, takes more time. In blood donations, the semi-quantitative gravimetric copper sulfate method which is very easy and inexpensive may be used but does not provide an acceptable degree of accuracy. The HemoCue® hemoglobin photometer has been used for these purposes. This study was conducted to generate data to support or refute its use as a point-of-care device for hemoglobin estimation in mobile blood donations and critical care areas in health facilities. Method EDTA blood was collected from study participants drawn from five groups: pre-school children, school children, pregnant women, non-pregnant women and men. Blood collected was immediately processed to estimate the hemoglobin concentration using three different methods (HemoCue®, Sysmex KX21N and Cyanmethemoglobin. Agreement between the test methods was assessed by the method of Bland and Altman. The Intraclass correlation coefficient (ICC was used to determine the within subject variability of measured hemoglobin. Results Of 398 subjects, 42% were males with the overall mean age being 19.4 years. The overall mean hemoglobin as estimated by each method was 10.4 g/dl for HemoCue, 10.3 g/dl for Sysmex KX21N and 10.3 g/dl for Cyanmethemoglobin. Pairwise analysis revealed that the hemoglobin determined by the HemoCue method was higher than that measured by the KX21N and Cyanmethemoglobin. Comparing the hemoglobin determined by the HemoCue to Cyanmethemoglobin, the concordance correlation coefficient was 0.995 (95% CI: 0.994-0.996, p Conclusion Hemoglobin determined by the HemoCue method is comparable to that determined by the other methods. The HemoCue photometer is therefore recommended for use as on-the-spot device for determining hemoglobin in resource poor setting.

Estimativa do sexo através de características métricas da mandíbula

OpenAIRE

Alves, Carina Filipa Pinto

2012-01-01

Dissertação de mestrado em Medicina (Medicina Legal e Ciências Forenses), apresentada à Faculdade de Medicina da Universidade de Coimbra A diagnose sexual a partir da análise de restos ósseos humanos é um elemento capital na investigação forense. Um grande número de estudos tem mostrado a existência de dimorfismo sexual em quase todos os ossos do esqueleto humano. A mandíbula humana adulta é um elemento do esqueleto particularmente robusto, sólido e usualmente preservado em contextos foren...

THE IMPACT OF SAINT PARASCHEVA PILGRIMAGE ON TOURISM IN IASI COUNTY

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Alexandra Georgiana PARASCA

2015-07-01

Full Text Available Nowadays, pilgrimage is a part of international tourism, being considered religious tourism, because it contributes to the development of economy, like any other type of tourism. This research paper takes into consideration the pilgrimage to Saint Paracheva, and how it helps the development of religious tourism in Iasi county, the economical implications. We try to capture the importance of Saint Parascheva pilgrimage, because year after year it bring many more pilgrims to Iasi city. In time, it could become an important place of pilgrimage at European level.

Determination Of Ph Including Hemoglobin Correction

Science.gov (United States)

Maynard, John D.; Hendee, Shonn P.; Rohrscheib, Mark R.; Nunez, David; Alam, M. Kathleen; Franke, James E.; Kemeny, Gabor J.

2005-09-13

Methods and apparatuses of determining the pH of a sample. A method can comprise determining an infrared spectrum of the sample, and determining the hemoglobin concentration of the sample. The hemoglobin concentration and the infrared spectrum can then be used to determine the pH of the sample. In some embodiments, the hemoglobin concentration can be used to select an model relating infrared spectra to pH that is applicable at the determined hemoglobin concentration. In other embodiments, a model relating hemoglobin concentration and infrared spectra to pH can be used. An apparatus according to the present invention can comprise an illumination system, adapted to supply radiation to a sample; a collection system, adapted to collect radiation expressed from the sample responsive to the incident radiation; and an analysis system, adapted to relate information about the incident radiation, the expressed radiation, and the hemoglobin concentration of the sample to pH.

Hemoglobin estimation by the HemoCue® portable hemoglobin photometer in a resource poor setting.

Science.gov (United States)

Nkrumah, Bernard; Nguah, Samuel Blay; Sarpong, Nimako; Dekker, Denise; Idriss, Ali; May, Juergen; Adu-Sarkodie, Yaw

2011-04-21

In resource poor settings where automated hematology analyzers are not available, the Cyanmethemoglobin method is often used. This method though cheaper, takes more time. In blood donations, the semi-quantitative gravimetric copper sulfate method which is very easy and inexpensive may be used but does not provide an acceptable degree of accuracy. The HemoCue® hemoglobin photometer has been used for these purposes. This study was conducted to generate data to support or refute its use as a point-of-care device for hemoglobin estimation in mobile blood donations and critical care areas in health facilities. EDTA blood was collected from study participants drawn from five groups: pre-school children, school children, pregnant women, non-pregnant women and men. Blood collected was immediately processed to estimate the hemoglobin concentration using three different methods (HemoCue®, Sysmex KX21N and Cyanmethemoglobin). Agreement between the test methods was assessed by the method of Bland and Altman. The Intraclass correlation coefficient (ICC) was used to determine the within subject variability of measured hemoglobin. Of 398 subjects, 42% were males with the overall mean age being 19.4 years. The overall mean hemoglobin as estimated by each method was 10.4 g/dl for HemoCue, 10.3 g/dl for Sysmex KX21N and 10.3 g/dl for Cyanmethemoglobin. Pairwise analysis revealed that the hemoglobin determined by the HemoCue method was higher than that measured by the KX21N and Cyanmethemoglobin. Comparing the hemoglobin determined by the HemoCue to Cyanmethemoglobin, the concordance correlation coefficient was 0.995 (95% CI: 0.994-0.996, p < 0.001). The Bland and Altman's limit of agreement was -0.389 - 0.644 g/dl with the mean difference being 0.127 (95% CI: 0.102-0.153) and a non-significant difference in variability between the two measurements (p = 0.843). After adjusting to assess the effect of other possible confounders such as sex, age and category of person, there was no

Nonlinear photoacoustic spectroscopy of hemoglobin

Energy Technology Data Exchange (ETDEWEB)

Danielli, Amos; Maslov, Konstantin; Favazza, Christopher P.; Xia, Jun; Wang, Lihong V., E-mail: LHWANG@WUSTL.EDU [Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, One Brookings Drive, St. Louis, Missouri 63130 (United States)

2015-05-18

As light intensity increases in photoacoustic imaging, the saturation of optical absorption and the temperature dependence of the thermal expansion coefficient result in a measurable nonlinear dependence of the photoacoustic (PA) signal on the excitation pulse fluence. Here, under controlled conditions, we investigate the intensity-dependent photoacoustic signals from oxygenated and deoxygenated hemoglobin at varied optical wavelengths and molecular concentrations. The wavelength and concentration dependencies of the nonlinear PA spectrum are found to be significantly greater in oxygenated hemoglobin than in deoxygenated hemoglobin. These effects are further influenced by the hemoglobin concentration. These nonlinear phenomena provide insights into applications of photoacoustics, such as measurements of average inter-molecular distances on a nm scale or with a tuned selection of wavelengths, a more accurate quantitative PA tomography.

Nonlinear photoacoustic spectroscopy of hemoglobin.

Science.gov (United States)

Danielli, Amos; Maslov, Konstantin; Favazza, Christopher P; Xia, Jun; Wang, Lihong V

2015-05-18

As light intensity increases in photoacoustic imaging, the saturation of optical absorption and the temperature dependence of the thermal expansion coefficient result in a measurable nonlinear dependence of the photoacoustic (PA) signal on the excitation pulse fluence. Here, under controlled conditions, we investigate the intensity-dependent photoacoustic signals from oxygenated and deoxygenated hemoglobin at varied optical wavelengths and molecular concentrations. The wavelength and concentration dependencies of the nonlinear PA spectrum are found to be significantly greater in oxygenated hemoglobin than in deoxygenated hemoglobin. These effects are further influenced by the hemoglobin concentration. These nonlinear phenomena provide insights into applications of photoacoustics, such as measurements of average inter-molecular distances on a nm scale or with a tuned selection of wavelengths, a more accurate quantitative PA tomography.

Nonlinear photoacoustic spectroscopy of hemoglobin

International Nuclear Information System (INIS)

Danielli, Amos; Maslov, Konstantin; Favazza, Christopher P.; Xia, Jun; Wang, Lihong V.

2015-01-01

As light intensity increases in photoacoustic imaging, the saturation of optical absorption and the temperature dependence of the thermal expansion coefficient result in a measurable nonlinear dependence of the photoacoustic (PA) signal on the excitation pulse fluence. Here, under controlled conditions, we investigate the intensity-dependent photoacoustic signals from oxygenated and deoxygenated hemoglobin at varied optical wavelengths and molecular concentrations. The wavelength and concentration dependencies of the nonlinear PA spectrum are found to be significantly greater in oxygenated hemoglobin than in deoxygenated hemoglobin. These effects are further influenced by the hemoglobin concentration. These nonlinear phenomena provide insights into applications of photoacoustics, such as measurements of average inter-molecular distances on a nm scale or with a tuned selection of wavelengths, a more accurate quantitative PA tomography

Hemoglobin Variants: Biochemical Properties and Clinical Correlates

Science.gov (United States)

Thom, Christopher S.; Dickson, Claire F.; Gell, David A.; Weiss, Mitchell J.

2013-01-01

Diseases affecting hemoglobin synthesis and function are extremely common worldwide. More than 1000 naturally occurring human hemoglobin variants with single amino acid substitutions throughout the molecule have been discovered, mainly through their clinical and/or laboratory manifestations. These variants alter hemoglobin structure and biochemical properties with physiological effects ranging from insignificant to severe. Studies of these mutations in patients and in the laboratory have produced a wealth of information on hemoglobin biochemistry and biology with significant implications for hematology practice. More generally, landmark studies of hemoglobin performed over the past 60 years have established important paradigms for the disciplines of structural biology, genetics, biochemistry, and medicine. Here we review the major classes of hemoglobin variants, emphasizing general concepts and illustrative examples. PMID:23388674

High affinity hemoglobin and Parkinson's disease.

Science.gov (United States)

Graham, Jeffrey; Hobson, Douglas; Ponnampalam, Arjuna

2014-12-01

Parkinson's disease (PD) is a neurodegenerative disorder characterized by the loss of dopaminergic neurons in the substantia nigra (SN) region of the midbrain. Oxidative damage in this region has been shown to play an important role in the pathogenesis of this disease. Human neurons have been discovered to contain hemoglobin, with an increased concentration seen in the neurons of the SN. High affinity hemoglobin is a clinical entity resulting from mutations that create a functional increase in the binding of hemoglobin to oxygen and an inability to efficiently unload it to tissues. This can result in a number of metabolic compensatory changes, including an elevation in circulating hemoglobin and an increase in the molecule 2,3-diphosphoglycerate (2,3-DPG). Population based studies have revealed that patients with PD have elevated hemoglobin as well as 2,3-DPG levels. Based on these observations, we hypothesize that the oxidative damage seen in PD is related to an underlying high affinity hemoglobin subtype. Copyright © 2014 Elsevier Ltd. All rights reserved.

Characteristic emission in glutaraldehyde polymerized hemoglobin

International Nuclear Information System (INIS)

Ma Li; Wang Xiaojun

2011-01-01

Hemoglobin with different modifications has been investigated using spectroscopic techniques. A new emission at around 371 nm has been observed under excitation of 305 nm from glutaraldehyde polymerized human hemoglobin. Intensity and peak position of the emission are dependent on both oxidation state and ligand environment and the emission has been identified from the hemoglobin oligomer.

On the Hierarchy of Saints on Altars. Visitation Records of Otto Friedrich Buchheim, the Bishop of Ljubljana (1641–1664

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Lavrič, Ana

2011-09-01

Full Text Available The visitation records of Otto Friedrich Buchheim, the Bishop of Ljubljana from 1641–1664, offer an insight into the rules governing the disposition of saintly figures in altar retables. The central place was accorded to the titular saint, while companion saints are positioned in pairs (separately for each level of the retable in such a way that those of higher rank are placed on the more distinguished gospel side, whereas those of lower rank stand on the subordinate epistle side. The priority of one saint over another was not a matter of a random choice, but of a fixed hierarchical order which was created over the course of centuries in the Litany of All Saints and also in the hymns of the officium for All Saints Day; this hierarchy, as Buchheim remarks, is "in agreement with the general feeling of the Catholic Church". Ecclesiastical art in Slovenia shows that in the Gothic period the hierarchical principle governing the disposition of saintly figures was not yet firmly fixed, but it was fully established in the late Renaissance and Baroque periods, which coincides with the period of unification of the Litany of All Saints for the entire Catholic Church. Later it started to loosen again, yet it remained in force up to the 20th century.

Radio-ligand immunoassay for human hemoglobin variants

International Nuclear Information System (INIS)

Javid, J.; Pettis, P.K.; Miller, J.E.

1981-01-01

A quantitative method is described for the individual assay of human hemoglobin variants occurring singly or in mixture. The hemoglobin to be assayed is bound to specific antibody; the immune complex is attached to protein A-containing S. aureus and removed from the mixture. The hemoglobin thus isolated is quantified by its ability to bind radiolabeled haptoglobin. The technique is accurate and distinguishes among the 4 hemoglobins tested, namely Hb A, S, C and F. It has the advantage over conventional radioimmunoassay that a single probe, radiolabeled haptoglobin, is needed for the specific assay of any hemoglobin. (Auth.)

Oxygen Measurements in Liposome Encapsulated Hemoglobin

Science.gov (United States)

Phiri, Joshua Benjamin

Liposome encapsulated hemoglobins (LEH's) are of current interest as blood substitutes. An analytical methodology for rapid non-invasive measurements of oxygen in artificial oxygen carriers is examined. High resolution optical absorption spectra are calculated by means of a one dimensional diffusion approximation. The encapsulated hemoglobin is prepared from fresh defibrinated bovine blood. Liposomes are prepared from hydrogenated soy phosphatidylcholine (HSPC), cholesterol and dicetylphosphate using a bath sonication method. An integrating sphere spectrophotometer is employed for diffuse optics measurements. Data is collected using an automated data acquisition system employing lock-in -amplifiers. The concentrations of hemoglobin derivatives are evaluated from the corresponding extinction coefficients using a numerical technique of singular value decomposition, and verification of the results is done using Monte Carlo simulations. In situ measurements are required for the determination of hemoglobin derivatives because most encapsulation methods invariably lead to the formation of methemoglobin, a nonfunctional form of hemoglobin. The methods employed in this work lead to high resolution absorption spectra of oxyhemoglobin and other derivatives in red blood cells and liposome encapsulated hemoglobin (LEH). The analysis using singular value decomposition method offers a quantitative means of calculating the fractions of oxyhemoglobin and other hemoglobin derivatives in LEH samples. The analytical methods developed in this work will become even more useful when production of LEH as a blood substitute is scaled up to large volumes.

Saint Petersburg magnetic observatory: from Voeikovo subdivision to INTERMAGNET certification

Science.gov (United States)

Sidorov, Roman; Soloviev, Anatoly; Krasnoperov, Roman; Kudin, Dmitry; Grudnev, Andrei; Kopytenko, Yury; Kotikov, Andrei; Sergushin, Pavel

2017-11-01

Since June 2012 the Saint Petersburg magnetic observatory is being developed and maintained by two institutions of the Russian Academy of Sciences (RAS) - the Geophysical Center of RAS (GC RAS) and the Saint Petersburg branch of the Pushkov Institute of Terrestrial Magnetism, Ionosphere and Radio Wave Propagation of RAS (IZMIRAN SPb). On 29 April 2016 the application of the Saint Petersburg observatory (IAGA code SPG) for introduction into the INTERMAGNET network was accepted after approval by the experts of the first definitive dataset over 2015, produced by the GC RAS, and on 9 June 2016 the SPG observatory was officially certified. One of the oldest series of magnetic observations, originating in 1834, was resumed in the 21st century, meeting the highest quality standards and all modern technical requirements. In this paper a brief historical and scientific background of the SPG observatory foundation and development is given, the stages of its renovation and upgrade in the 21st century are described, and information on its current state is provided. The first results of the observatory functioning are discussed and geomagnetic variations registered at the SPG observatory are assessed and compared with geomagnetic data from the INTERMAGNET observatories located in the same region.

Saint Petersburg magnetic observatory: from Voeikovo subdivision to INTERMAGNET certification

Directory of Open Access Journals (Sweden)

R. Sidorov

2017-11-01

Full Text Available Since June 2012 the Saint Petersburg magnetic observatory is being developed and maintained by two institutions of the Russian Academy of Sciences (RAS – the Geophysical Center of RAS (GC RAS and the Saint Petersburg branch of the Pushkov Institute of Terrestrial Magnetism, Ionosphere and Radio Wave Propagation of RAS (IZMIRAN SPb. On 29 April 2016 the application of the Saint Petersburg observatory (IAGA code SPG for introduction into the INTERMAGNET network was accepted after approval by the experts of the first definitive dataset over 2015, produced by the GC RAS, and on 9 June 2016 the SPG observatory was officially certified. One of the oldest series of magnetic observations, originating in 1834, was resumed in the 21st century, meeting the highest quality standards and all modern technical requirements. In this paper a brief historical and scientific background of the SPG observatory foundation and development is given, the stages of its renovation and upgrade in the 21st century are described, and information on its current state is provided. The first results of the observatory functioning are discussed and geomagnetic variations registered at the SPG observatory are assessed and compared with geomagnetic data from the INTERMAGNET observatories located in the same region.

Relationship of Baseline Hemoglobin Level with Serum Ferritin, Postphlebotomy Hemoglobin Changes, and Phlebotomy Requirements among HFE C282Y Homozygotes

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Seyed Ali Mousavi

2015-01-01

Full Text Available Objectives. We aimed to examine whether baseline hemoglobin levels in C282Y-homozygous patients are related to the degree of serum ferritin (SF elevation and whether patients with different baseline hemoglobin have different phlebotomy requirements. Methods. A total of 196 patients (124 males and 72 females who had undergone therapeutic phlebotomy and had SF and both pre- and posttreatment hemoglobin values were included in the study. Results. Bivariate correlation analysis suggested that baseline SF explains approximately 6 to 7% of the variation in baseline hemoglobin. The results also showed that males who had higher (≥150 g/L baseline hemoglobin levels had a significantly greater reduction in their posttreatment hemoglobin despite requiring fewer phlebotomies to achieve iron depletion than those who had lower (<150 g/L baseline hemoglobin, regardless of whether baseline SF was below or above 1000 µg/L. There were no significant differences between hemoglobin subgroups regarding baseline and treatment characteristics, except for transferrin saturation between male subgroups with SF above 1000 µg/L. Similar differences were observed when females with higher (≥138 g/L baseline hemoglobin were compared with those with lower (<138 g/L baseline hemoglobin. Conclusion. Dividing C282Y-homozygous patients into just two subgroups according to the degree of baseline SF elevation may obscure important subgroup variations.

Relationship of Baseline Hemoglobin Level with Serum Ferritin, Postphlebotomy Hemoglobin Changes, and Phlebotomy Requirements among HFE C282Y Homozygotes

Science.gov (United States)

Mousavi, Seyed Ali; Mahmood, Faiza; Aandahl, Astrid; Knutsen, Teresa Risopatron; Llohn, Abid Hussain

2015-01-01

Objectives. We aimed to examine whether baseline hemoglobin levels in C282Y-homozygous patients are related to the degree of serum ferritin (SF) elevation and whether patients with different baseline hemoglobin have different phlebotomy requirements. Methods. A total of 196 patients (124 males and 72 females) who had undergone therapeutic phlebotomy and had SF and both pre- and posttreatment hemoglobin values were included in the study. Results. Bivariate correlation analysis suggested that baseline SF explains approximately 6 to 7% of the variation in baseline hemoglobin. The results also showed that males who had higher (≥150 g/L) baseline hemoglobin levels had a significantly greater reduction in their posttreatment hemoglobin despite requiring fewer phlebotomies to achieve iron depletion than those who had lower (baseline hemoglobin, regardless of whether baseline SF was below or above 1000 µg/L. There were no significant differences between hemoglobin subgroups regarding baseline and treatment characteristics, except for transferrin saturation between male subgroups with SF above 1000 µg/L. Similar differences were observed when females with higher (≥138 g/L) baseline hemoglobin were compared with those with lower (baseline hemoglobin. Conclusion. Dividing C282Y-homozygous patients into just two subgroups according to the degree of baseline SF elevation may obscure important subgroup variations. PMID:26380265

Thinking with the saint: the miracle of Saint Januarius of Naples and science in early modern Europe.

Science.gov (United States)

de Ceglia, Francesco Paolo

2014-01-01

The aim of this paper is to reconstruct the way in which early modem science questioned and indirectly influenced (while being in its turn influenced by) the conceptualization of the liquefaction of the blood of Saint Januarius, a phenomenon that has been taking place at regular intervals in Naples since the late Middle Ages. In the seventeenth century, a debate arose that divided Europe between supporters of a theory of divine intervention and believers in the occult properties of the blood. These two theoretical options reflected two different perspectives on the relationship between the natural and the supernatural. While in the seventeenth century, the emphasis was placed on the predictable periodicity of the miraculous event of liquefaction as a manifestation of God in his role as a divine regulator, in the eighteenth century the event came to be described as capricious and unpredictable, in an attempt to differentiate miracles from the workings of nature, which were deemed to be normative. The miracle of the blood of Saint Januarius thus provides a window through which we can catch a glimpse of how the natural order was perceived in early modern Europe at a time when the Continent was culturally fragmented into north and south, Protestantism and Catholicism, learned and ignorant.

A review of variant hemoglobins interfering with hemoglobin A1c measurement.

Science.gov (United States)

Little, Randie R; Roberts, William L

2009-05-01

Hemoglobin A1c (HbA1c) is used routinely to monitor long-term glycemic control in people with diabetes mellitus, as HbA1c is related directly to risks for diabetic complications. The accuracy of HbA1c methods can be affected adversely by the presence of hemoglobin (Hb) variants or elevated levels of fetal hemoglobin (HbF). The effect of each variant or elevated HbF must be examined with each specific method. The most common Hb variants worldwide are HbS, HbE, HbC, and HbD. All of these Hb variants have single amino acid substitutions in the Hb beta chain. HbF is the major hemoglobin during intrauterine life; by the end of the first year, HbF falls to values close to adult levels of approximately 1%. However, elevated HbF levels can occur in certain pathologic conditions or with hereditary persistence of fetal hemoglobin. In a series of publications over the past several years, the effects of these four most common Hb variants and elevated HbF have been described. There are clinically significant interferences with some methods for each of these variants. A summary is given showing which methods are affected by the presence of the heterozygous variants S, E, C, and D and elevated HbF. Methods are divided by type (immunoassay, ion-exchange high-performance liquid chromatography, boronate affinity, other) with an indication of whether the result is artificially increased or decreased by the presence of a Hb variant. Laboratorians should be aware of the limitations of their method with respect to these interferences. 2009 Diabetes Technology Society.

Virological Surveillance of Dengue in Saint Martin and Saint Barthélemy, French West Indies, Using Blood Samples on Filter Paper

Science.gov (United States)

Matheus, Séverine; Chappert, Jean-Loup; Cassadou, Sylvie; Berger, Franck; Labeau, Bhetty; Bremand, Laetitia; Winicki, Alain; Huc-Anais, Patricia; Quenel, Philippe; Dussart, Philippe

2012-01-01

To strengthen active dengue surveillance in Saint Martin and Saint Barthélemy, two French Caribbean islands, we evaluated the epidemiological usefulness of collecting blood samples from NS1-positive dengue patients on filter paper to identify the dengue serotypes circulating in these regions during a 27-month period. This approach allowed dengue serotypes to be identified by reverse transcriptase-polymerase chain reaction in 90.1% of the total set of 666 samples analyzed and, in 95.5% of the samples collected during the acute phase of the disease. This prospective virological surveillance using blood samples absorbed onto filter paper, which were stored at 4°C and shipped at ambient temperature to a specialized laboratory for analysis, allowed us to avoid the logistic and financial costs associated with shipping frozen venous blood samples. This surveillance system offers a low-cost alternative for reinforcing dengue prevention in areas where specialized laboratories do not exist, notably by facilitating the early detection of potentially new dengue serotypes. PMID:22232467

MARKETING STRATEGY FOR THE FINNISH TOURIST COMPANY BASED ON SAINT PETERSBURG MARKET RESEARCH

OpenAIRE

Miroshnichenko, Julia

2014-01-01

The goal of this thesis is to get a marketing strategy for proposed tourist business through the field-work marketing research. The purpose of this marketing research was to investigate the attitudes of Russian tourists in Saint Pe-tersburg towards Finland as a destination. The research examines the respondents’ own evaluation of the Finnish tourism possibilities, as well as the expert evaluation (made by the professionals of tourist agencies in Saint Petersburg) of the Finnish tourist ma...

Characterization of hemoglobin-benzo[a]pyrene adducts

International Nuclear Information System (INIS)

Haugen, D.A.; Myers, S.R.

1987-01-01

Cultures of Syrian hamster embryo (SHE) cells were supplemented with human Hb (0.2 mM heme) and [ 3 H]BP (1 μM). After a 24-h incubation, the medium was removed and subjected to cation-exchange liquid chromatography (CM-Sepharose) to resolve hemoglobins from serum proteins in the medium. The BP-treated Hb was subjected to analysis in each of three column chromatographic systems established for isolation and characterization of human hemoglobin and its genetic and post-translationally modified variants. Results demonstrate that hemoglobin-carcinogen adducts can be resolved from native hemoglobin by established conventional and high-performance liquid chromatographic procedures, suggesting the basis for development of general approaches for isolating and characterizing hemoglobin-carcinogen adducts. The results also suggest the basis for a model system in which adducts between carcinogens and human hemoglobin are formed in cultures of mammalian cells or tissues

A Latter-day Saint Approach to Addiction: Aetiology, Consequences and Treatment in a Theological Context

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James D. Holt

2014-12-01

Full Text Available This article explores the theological underpinning of the nature, aetiology and treatment of addictions within The Church of Jesus Christ of Latter-day Saints. The first section outlines the “plan of salvation” and how this provides the theological framework for the source and solution to addictions. The final section explores addiction against this background in terms of its aetiology, types, consequences and treatment in a Latter-day Saint context. In so doing it builds on the recognition by the Church in recent years that addiction is a problem in the lives of some of its members and that treatment programs coherent with its teachings and beliefs are necessary. The article concludes by suggesting that while addiction may be more openly discussed within a Latter-day Saint context there is a need to keep this dialogue moving forward. This article does not examine Latter-day Saint teaching within the wider context of psychotherapy and other definitions of addiction; rather it explores the place of addiction as understood within the theological and ecclesiological context of Mormonism.

The Forging of a White Gay Aesthetic at the Saint, 1980–84

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Tim Lawrence

2011-06-01

Full Text Available Based on original interviews with key protagonists and documentary research, the article examines the way in which the DJs who worked at the Saint forged a white gay aesthetic across the first half of the 1980s. A private party located in the East Village, New York, the Saint attracted a privileged white male crowd, and this group's position within the emerging culture of neoliberalism, along with the deepening impact of the AIDS epidemic, encouraged its spinners to sever their ties with sounds that were associated with blackness. However, if Saint DJs rejected a high proportion of records that were being released by New York's independents, and also snubbed Chicago house when it broke in 1985, their forging of an aesthetic that emphasised seamless transitions, often between records that contained similar sonic qualities, preempted the style of mixing that would be popularised in house music culture.

Contextualising and Visualising Saints in the 14-15th century

Czech Academy of Sciences Publication Activity Database

Horníčková, Kateřina

-, č. 62 (2011), s. 21-39 ISSN 1029-0737 Institutional research plan: CEZ:AV0Z90090514 Keywords : medieval art * saints' representation * representation of place Subject RIV: AA - Philosophy ; Religion

New observations on the miniature of the vision of Saint Gregory of Nazianzus in Paris. GR. 510

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Gavrilović Zaga

2007-01-01

Full Text Available The article deals with the iconography of the illustration of the Second Paschal Homily of St. Gregory of Nazianzus on fol. 285r of the Paris manuscript. It questions the identity of the woman saint represented on the right of St. Paraskeve in the lower register of the scene. Unlike that above St. Paraskeve, the inscription identifying this second woman saint is fragmentary and difficult to read, but it has been widely accepted that she is Saint Helena, mother of the Emperor Constantine the Great. On the basis of two other representations of Helena in the same manuscript and of the style of the inscription accompanying them, as well as taking into account the importance of the theological meaning expounded by St. Gregory in his oration, it is suggested that the second woman saint may be St. Kyriake.

De Manet a Yves Saint-Laurent: uma análise semiótica

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Christiane Paula Godinho Santarelli

2008-09-01

Full Text Available A proposta deste artigo é fazer uma análise semiótica, com base na teoria greimasiana, da pintura original de Manet Le déjeuner sur l’herbe e de sua recriação para o anúncio da marca Yves Saint-Laurent. Os aspectos analisados nos dois “textos” são: os sistemas de valores, a estrutura narrativa, a estrutura de poder, os objetos de valor e a semântica profunda. A comparação dos dois discursos tem como objetivo atentar para as contradições no suposto discurso libertador da publicidade de Yves Saint-Laurent, que pode estar presente na publicidade de outras marcas. Palavras-chave: Publicidade; semiótica; arte; moda. ABSTRACT The purpose of this article is to offer a semiotical analysis of the original painting of Manet Le déjeuner sur l’herbe and of its recreation in an advertisement for the brand Yves Saint-Laurent. The aspects analyzed in the two “texts” are: the systems of enclosed values, the narrative structure, the power structure, the objects of value and deep semantics. The comparison between the two forms of speech aims to highlight the contradictions in the alleged liberating speech of the Yves Saint-Laurent advertisement, which may be present in advertising pieces from other brands. Keywords: Advertising; semiotics; art; fashion.

Utilidad de la Ortopantografía vs TAC facial en el diagnóstico de fracturas de mandíbula

Directory of Open Access Journals (Sweden)

M. P. Casteleiro Roca

Full Text Available Nuestro objetivo es conocer la utilidad de la Ortopantografía frente a la TAC (Tomografía Axial Computarizada para el diagnóstico de las fracturas de mandíbula, pretendiendo implantar en nuestro hospital recomendaciones basadas en la evidencia. Llevamos a cabo una revisión sistemática en PubMed: la estrategia de búsqueda se define como "Jaw Fractures" [MeSH] AND "Tomography, X-Ray Computed" [MeSH], restringiendo dicha búsqueda a los últimos 5 años (2001-2005 y limitándola a inglés y español. Los criterios de inclusión establecidos son: diseños de estudio, revisiones sistemáticas, meta-análisis, estudios comparando utilidad de Ortopantografía frente a TAC en diagnóstico de fracturas mandibulares. Como criterios de exclusión marcamos: casos clínicos, cartas al director, no comparar Ortopantografía frente a TAC en diagnóstico de fracturas mandibulares. Obtenemos 41 artículos que analizamos y clasificamos aplicando los criterios descritos y obteniendo finalmente 3 artículos. Actualmente, como prueba única, la TAC proporciona más información diagnóstica que la Ortopantografía en las fracturas de mandíbula.

MR Imaging-derived Oxygen-Hemoglobin Dissociation Curves and Fetal-Placental Oxygen-Hemoglobin Affinities.

Science.gov (United States)

Avni, Reut; Golani, Ofra; Akselrod-Ballin, Ayelet; Cohen, Yonni; Biton, Inbal; Garbow, Joel R; Neeman, Michal

2016-07-01

Purpose To generate magnetic resonance (MR) imaging-derived, oxygen-hemoglobin dissociation curves and to map fetal-placental oxygen-hemoglobin affinity in pregnant mice noninvasively by combining blood oxygen level-dependent (BOLD) T2* and oxygen-weighted T1 contrast mechanisms under different respiration challenges. Materials and Methods All procedures were approved by the Weizmann Institutional Animal Care and Use Committee. Pregnant mice were analyzed with MR imaging at 9.4 T on embryonic days 14.5 (eight dams and 58 fetuses; imprinting control region ICR strain) and 17.5 (21 dams and 158 fetuses) under respiration challenges ranging from hyperoxia to hypoxia (10 levels of oxygenation, 100%-10%; total imaging time, 100 minutes). A shorter protocol with normoxia to hyperoxia was also performed (five levels of oxygenation, 20%-100%; total imaging time, 60 minutes). Fast spin-echo anatomic images were obtained, followed by sequential acquisition of three-dimensional gradient-echo T2*- and T1-weighted images. Automated registration was applied to align regions of interest of the entire placenta, fetal liver, and maternal liver. Results were compared by using a two-tailed unpaired Student t test. R1 and R2* values were derived for each tissue. MR imaging-based oxygen-hemoglobin dissociation curves were constructed by nonlinear least square fitting of 1 minus the change in R2*divided by R2*at baseline as a function of R1 to a sigmoid-shaped curve. The apparent P50 (oxygen tension at which hemoglobin is 50% saturated) value was derived from the curves, calculated as the R1 scaled value (x) at which the change in R2* divided by R2*at baseline scaled (y) equals 0.5. Results The apparent P50 values were significantly lower in fetal liver than in maternal liver for both gestation stages (day 14.5: 21% ± 5 [P = .04] and day 17.5: 41% ± 7 [P hemoglobin dissociation curves with a shorter protocol that excluded the hypoxic periods was demonstrated. Conclusion MR imaging

Hemoglobin of mice with radiation-induced mutations at the hemoglobin loci

Energy Technology Data Exchange (ETDEWEB)

Popp, R A; Stratton, L P; Hawley, D K; Effron, K [Oak Ridge National Lab., TN (USA)

1979-01-15

Chemical analyses were done on the abnormal hemoglobins of the five (101 x SEC)F/sub 1/ offspring of X- irradiated adult SEC mice to determine which hemoglobin genes were expressed in each hemoglobin variant. Three offspring of irradiated SEC males did not express either of the two kinds of ..cap alpha..-chains normally found in all SEC mice. The deficient ..cap alpha..-chain synthesis caused these mice to exhibit an ..cap alpha..-thalassemia similar to human ..cap alpha..-thalassemia. Scanning electron microscopy was used to show that many erythrocytes of mice with ..cap alpha..-thalassemia have bizarre shapes; e.g. many erythrocytes appeared flattened or had thorny projections (acanthocytes). One mutant with a tandem duplication of a segment of chromosome 7 (site of locus determining ..beta..-chain structure) produced twice as much SEC as 101 ..beta..-chain polypeptides. One mutant that probably arose by non-disjunction of chromosome 7's in its unirradiated 101 mother and loss of chromosome 7 from the gamete of its irradiated SEC father did not express the SEC ..beta..-chain gene.

Hemoglobin of mice with radiation-induced mutations at the hemoglobin loci

International Nuclear Information System (INIS)

Popp, R.A.; Stratton, L.P.; Hawley, D.K.; Effron, K.

1979-01-01

Chemical analyses were done on the abnormal hemoglobins of the five (101 x SEC)F 1 offspring of X- irradiated adult SEC mice to determine which hemoglobin genes were expressed in each hemoglobin variant. Three offspring of irradiated SEC males did not express either of the two kinds of α-chains normally found in all SEC mice. The deficient α-chain synthesis caused these mice to exhibit an α-thalassemia similar to human α-thalassemia. Scanning electron microscopy was used to show that many erythrocytes of mice with α-thalassemia have bizarre shapes; e.g. many erythrocytes appeared flattened or had thorny projections (acanthocytes). One mutant with a tandem duplication of a segment of chromosome 7 (site of locus determining β-chain structure) produced twice as much SEC as 101 β-chain polypeptides. One mutant that probably arose by non-disjunction of chromosome 7's in its unirradiated 101 mother and loss of chromosome 7 from the gamete of its irradiated SEC father did not express the SEC β-chain gene. (author)

Effect of Multiple Mutations in the Hemoglobin- and Hemoglobin-Haptoglobin-Binding Proteins, HgpA, HgpB, and HgpC, of Haemophilus influenzae Type b

OpenAIRE

Morton, Daniel J.; Whitby, Paul W.; Jin, Hongfan; Ren, Zhen; Stull, Terrence L.

1999-01-01

Haemophilus influenzae requires heme for growth and can utilize hemoglobin and hemoglobin-haptoglobin as heme sources. We previously identified two hemoglobin- and hemoglobin-haptoglobin-binding proteins, HgpA and HgpB, in H. influenzae HI689. Insertional mutation of hgpA and hgpB, either singly or together, did not abrogate the ability to utilize or bind either hemoglobin or the hemoglobin-haptoglobin complex. A hemoglobin affinity purification method was used to isolate a protein of approxi...

Impact of bacteriophage Saint3 carriage on the immune evasion capacity and hemolytic potential of Staphylococcus aureus CC398.

Science.gov (United States)

Jung, Philipp; Abdelbary, Mohamed M H; Kraushaar, Britta; Fetsch, Alexandra; Geisel, Jürgen; Herrmann, Mathias; Witte, Wolfgang; Cuny, Christiane; Bischoff, Markus

2017-02-01

Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) isolates of clonal complex 398 (CC398) are frequently found in Europe, and recent studies highlighted the importance of mobile genetic element (MGE) exchange for host adaptation of this lineage. Of note, one of the MGEs commonly found in human S. aureus isolates, the immune evasion cluster (IEC) harboring bacteriophage Saint3, is very rarely found in LA-MRSA CC398 isolates obtained from farm animals, but more frequently found in LA-MRSA CC398 that were retransmitted to humans. Here, we analyzed with a set of S. aureus CC398 isolates harboring/lacking φSaint3 how this MGE affects (i) phagocytosis of CC398 isolates by polymorphonuclear neutrophils (PMNs), and (ii) hemolysis of human and livestock-derived erythrocytes. Isolates lacking φSaint3 were more efficiently phagocytosed by human PMNs in whole blood phagocytosis assays than isolates harboring this bacteriophage, irrespective of their origin. Notably, a similar effect was observed when equine blood was utilized, but not detected with porcine blood. Integration of φSaint3 into LA-MRSA CC398 strains lacking this MGE confirmed these findings, as φSaint3-harboring recipients were again less efficiently ingested by PMNs in equine and human blood than their parental strains. Integration of φSaint3 strongly reduced the hemolytic potential of the culture supernatants against human-derived erythrocytes, and to a smaller extent also against porcine-derived erythrocytes, while φSaint3 integration only slightly affected the hemolytic capacities against equine-derived red blood cells. The significant protective effect of φSaint3 against phagocytosis by equine PMNs suggests that the host specificity of the IEC components might be broader than currently assumed. Copyright © 2016 Elsevier B.V. All rights reserved.

Led Astray by Hemoglobin A1c

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Jean Chen MD

2016-01-01

Full Text Available Hemoglobin A1c (A1c is used frequently to diagnose and treat diabetes mellitus. Therefore, it is important be aware of factors that may interfere with the accuracy of A1c measurements. This is a case of a rare hemoglobin variant that falsely elevated a nondiabetic patient’s A1c level and led to a misdiagnosis of diabetes. A 67-year-old male presented to endocrine clinic for further management after he was diagnosed with diabetes based on an elevated A1c of 10.7%, which is approximately equivalent to an average blood glucose of 260 mg/dL. Multiple repeat A1c levels remained >10%, but his home fasting and random glucose monitoring ranged from 92 to 130 mg/dL. Hemoglobin electrophoresis and subsequent genetic analysis diagnosed the patient with hemoglobin Wayne, a rare hemoglobin variant. This variant falsely elevates A1c levels when A1c is measured using cation-exchange high-performance liquid chromatography. When the boronate affinity method was applied instead, the patient’s A1c level was actually 4.7%. Though hemoglobin Wayne is clinically silent, this patient was erroneously diagnosed with diabetes and started on an antiglycemic medication. Due to this misdiagnosis, the patient was at risk of escalation in his “diabetes management” and hypoglycemia. Therefore, it is important that providers are aware of factors that may result in hemoglobin A1c inaccuracy including hemoglobin variants.

Moessbauer study of hemoglobin of diabetes

International Nuclear Information System (INIS)

Li Aiguo; Ni Xinbo; Cai Yingwen; Zhang Guilin; Zhang Hongde; Ge Yongxin

2000-01-01

The hemoglobins from normal adults (Gly-Hb 5%), people infected with diabetes (Gly-Hb 10%) and serious diabetics (Gly-Hb 15%) were investigated by Moessbauer spectroscopy at liquid nitrogen temperature. All the experimental spectra of hemoglobin are composed of three doublets corresponding to oxy-hemoglobin (Oxy-Hb), deoxy-hemoglobin (Deoxy-Hb) and low-spin hemo-chrome (Ls-Hemo) respectively. It is found that Oxy-Hb is decreasing but Deoxy-hb increasing for diabetes. Experimental results also indicate that the line-width of Moessbauer spectra of Oxy-Hb for diabetics is narrower than that for normal adults, showing that while Fe on Oxy-Hb exists in pile-up of some similar states for normal adults, but it becomes in single state for serious diabetes

Orthokeratinized odontogenic cyst: a report of two cases in the mandible = Cisto odontogênico ortoqueratinizado: relato de dois casos em mandíbula

Directory of Open Access Journals (Sweden)

Pereira, Francisco de Assis Caldas

2012-01-01

Conclusão: Cisto odontogênico ortoqueratinizado deve ser considerado no diagnóstico diferencial das lesões que ocorrem na mandíbula associado com dentes impactados, especialmente aqueles casos que simulem o cisto dentígero. Além disso, observamos a importância de tomadas radiográficas antes do tratamento ortodôntico no diagnóstico de patologias bucais

Two-photon excited fluorescence emission from hemoglobin

Science.gov (United States)

Sun, Qiqi; Zeng, Yan; Zhang, Wei; Zheng, Wei; Luo, Yi; Qu, Jianan Y.

2015-03-01

Hemoglobin, one of the most important proteins in blood, is responsible for oxygen transportation in almost all vertebrates. Recently, we discovered two-photon excited hemoglobin fluorescence and achieved label-free microvascular imaging based on the hemoglobin fluorescence. However, the mechanism of its fluorescence emission still remains unknown. In this work, we studied the two-photon excited fluorescence properties of the hemoglobin subunits, heme/hemin (iron (II)/(III) protoporphyrin IX) and globin. We first studied the properties of heme and the similar spectral and temporal characteristics of heme and hemoglobin fluorescence provide strong evidence that heme is the fluorophore in hemoglobin. Then we studied the fluorescence properties of hemin, globin and methemoglobin, and found that the hemin may have the main effect on the methemoglobin fluorescence and that globin has tryptophan fluorescence like other proteins. Finally, since heme is a centrosymmetric molecule, that the Soret band fluorescence of heme and hemoglobin was not observed in the single photon process in the previous study may be due to the parity selection rule. The discovery of heme two-photon excited fluorescence may open a new window for heme biology research, since heme as a cofactor of hemoprotein has many functions, including chemical catalysis, electron transfer and diatomic gases transportation.

Geologic map of Saint Lawrence Island, Alaska

Science.gov (United States)

Patton, William W.; Wilson, Frederic H.; Taylor, Theresa A.

2011-01-01

Saint Lawrence Island is located in the northern Bering Sea, 190 km southwest of the tip of the Seward Peninsula, Alaska, and 75 km southeast of the Chukotsk Peninsula, Russia (see index map, map sheet). It lies on a broad, shallow-water continental shelf that extends from western Alaska to northeastern Russia. The island is situated on a northwest-trending structural uplift exposing rocks as old as Paleozoic above sea level. The submerged shelf between the Seward Peninsula and Saint Lawrence Island is covered mainly with Cenozoic deposits (Dundo and Egiazarov, 1982). Northeast of the island, the shelf is underlain by a large structural depression, the Norton Basin, which contains as much as 6.5 km of Cenozoic strata (Grim and McManus, 1970; Fisher and others, 1982). Sparse test-well data indicate that the Cenozoic strata are underlain by Paleozoic and Proterozoic rocks, similar to those exposed on the Seward Peninsula (Turner and others, 1983). Saint Lawrence Island is 160 km long in an east-west direction and from 15 km to 55 km wide in a north-south direction. The east end of the island consists largely of a wave-cut platform, which has been elevated as much as 30 m above sea level. Isolated upland areas composed largely of granitic plutons rise as much as 550 m above the wave-cut platform. The central part of the island is dominated by the Kookooligit Mountains, a large Quaternary shield volcano that extends over an area of 850 km2 and rises to an elevation of 630 m. The west end of the island is composed of the Poovoot Range, a group of barren, rubble-covered hills as high as 450 m that extend from Boxer Bay on the southwest coast to Taphook Mountain on the north coast. The Poovoot Range is flanked on the southeast by the Putgut Plateau, a nearly flat, lake-dotted plain that stands 30?60 m above sea level. The west end of the island is marked by uplands underlain by the Sevuokuk pluton (unit Kg), a long narrow granite body that extends from Gambell on the

Convergent evolution of hemoglobin switching in jawed and jawless vertebrates.

Science.gov (United States)

Rohlfing, Kim; Stuhlmann, Friederike; Docker, Margaret F; Burmester, Thorsten

2016-02-01

During development, humans and other jawed vertebrates (Gnathostomata) express distinct hemoglobin genes, resulting in different hemoglobin tetramers. Embryonic and fetal hemoglobin have higher oxygen affinities than the adult hemoglobin, sustaining the oxygen demand of the developing organism. Little is known about the expression of hemoglobins during development of jawless vertebrates (Agnatha). We identified three hemoglobin switches in the life cycle of the sea lamprey. Three hemoglobin genes are specifically expressed in the embryo, four genes in the filter feeding larva (ammocoete), and nine genes correspond to the adult hemoglobin chains. During the development from the parasitic to the reproductive adult, the composition of hemoglobin changes again, with a massive increase of chain aHb1. A single hemoglobin chain is expressed constitutively in all stages. We further showed the differential expression of other globin genes: Myoglobin 1 is most highly expressed in the reproductive adult, myoglobin 2 expression peaks in the larva. Globin X1 is restricted to the embryo; globin X2 was only found in the reproductive adult. Cytoglobin is expressed at low levels throughout the life cycle. Because the hemoglobins of jawed and jawless vertebrates evolved independently from a common globin ancestor, hemoglobin switching must also have evolved convergently in these taxa. Notably, the ontogeny of sea lamprey hemoglobins essentially recapitulates their phylogeny, with the embryonic hemoglobins emerging first, followed by the evolution of larval and adult hemoglobins.

The implantation of a PV electric energy generation system at the Saint Peter and Saint Paul islands

Energy Technology Data Exchange (ETDEWEB)

Galdino, Marco Antonio; Silva, Patricia de Castro da [Centro de Pesquisas de Energia Eletrica (CEPEL), Rio de Janeiro, RJ (Brazil)], E-mail: marcoag@cepel.br; Souza, Marco Antonio Carvalho [Secretaria da Comissao Interministerial para os Recursos do Mar (SECIRM), Brasilia, DF (Brazil)

2009-07-01

The Saint Peter and Saint Paul Islands are located at 0 degree 55.00' N and 29 degree 20.76'' W, at a distance of circa 550 M (nautical miles) NE from the City of Natal, RN (Northeast Region of Brazil), and comprises of many small islands and rocks of igneous plutonic origin. In 1998, a first Scientific Station was built in order to receive researchers involved in several projects. The CEPEL, as responsible by the electrical energy supply to the Scientific Station; designed and installed a PV electric energy generation system which had a power of 3.6 kWp. This system operated successfully for the last 10 years, suffering frequent maintenance. In 2006, a new design for the Scientific Station has been started aiming to improve its resources and safety. The new PV system has a maximum power of 7.8 kWp, and employs an updated technology (SMA, Germany). The equipment was integrated and submitted to intensive testing at facilities of CEPEL, and was installed and commissioned at the Islands in June 2008. Since the installation, the equipment has been operating as required, meeting the energy and water demand of the Station. The present paper describes the many steps involved in the implantation of the PV system at the Scientific Station. (author)

Small angle X-ray scattering on concentrated hemoglobin solutions

International Nuclear Information System (INIS)

Zinke, M.; Damaschun, G.; Mueller, J.J.; Ruckpaul, K.

1978-01-01

The small-angle X-ray scattering technique was used to determine the intermolecular structure and interaction potentials in oxi-and deoxi-hemoglobin solutions. The pair correlation function obtained by the ZERNICKE-PRINS equation characterizes the intermolecular structure of the hemoglobin molecules. The intermolecular structure is concentration dependent. The hemoglobin molecules have a 'short range order structure' with a range of about 4 molecule diameters at 324 g/l. The potential functions of the hemoglobin-hemoglobin interaction have been determined on the basis of fluid theories. Except for the deoxi-hemoglobin solution having the concentration 370 g/l, the pair interaction consists in a short repulsion and a weak short-range attraction against kT. The potential minimum is between 1.2 - 1.5 nm above the greatest hemoglobin diameter. (author)

Spectroscopic study of gamma irradiated bovine hemoglobin

International Nuclear Information System (INIS)

Maghraby, Ahmed Mohamed; Ali, Maha Anwar

2007-01-01

In the present study, the effects of ionizing radiation of Cs-137 and Co-60 from 4.95 to 743.14 Gy and from 40 Gy to 300 kGy, respectively, on some bovine hemoglobin characteristics were studied. Such an effect was evaluated using electron paramagnetic resonance (EPR) spectroscopy, and infra-red (IR) spectroscopy. Bovine hemoglobin EPR spectra were recorded and analyzed before and after irradiation and changes were explained in detail. IR spectra of unirradiated and irradiated Bovine hemoglobin were recorded and analyzed also. It was found that ionizing radiation may lead to the increase of free radicals production, the decrease in α-helices contents, which reflects the degradation of hemoglobin molecular structure, or at least its incomplete performance. Results also show that the combined application of EPR and FTIR spectroscopy is a powerful tool for determining structural modification of bovine hemoglobin samples exposed to gamma irradiation

Genetic hemoglobin disorders rather than iron deficiency are a major predictor of hemoglobin concentration in women of reproductive age in rural prey Veng, Cambodia.

Science.gov (United States)

Karakochuk, Crystal D; Whitfield, Kyly C; Barr, Susan I; Lamers, Yvonne; Devlin, Angela M; Vercauteren, Suzanne M; Kroeun, Hou; Talukder, Aminuzzaman; McLean, Judy; Green, Timothy J

2015-01-01

Anemia is common in Cambodian women. Potential causes include micronutrient deficiencies, genetic hemoglobin disorders, inflammation, and disease. We aimed to investigate factors associated with anemia (low hemoglobin concentration) in rural Cambodian women (18-45 y) and to investigate the relations between hemoglobin disorders and other iron biomarkers. Blood samples were obtained from 450 women. A complete blood count was conducted, and serum and plasma were analyzed for ferritin, soluble transferrin receptor (sTfR), folate, vitamin B-12, retinol binding protein (RBP), C-reactive protein (CRP), and α1 acid glycoprotein (AGP). Hemoglobin electrophoresis and multiplex polymerase chain reaction were used to determine the prevalence and type of genetic hemoglobin disorders. Overall, 54% of women had a genetic hemoglobin disorder, which included 25 different genotypes (most commonly, hemoglobin E variants and α(3.7)-thalassemia). Of the 420 nonpregnant women, 29.5% had anemia (hemoglobin 8.3 mg/L), hemoglobin disorders, respectively. There was no biochemical evidence of vitamin A deficiency (RBP 5 mg/L) and 26% (AGP >1 g/L) of nonpregnant women, respectively. By using an adjusted linear regression model, the strongest predictors of hemoglobin concentration were hemoglobin E homozygous disorder and pregnancy status. Other predictors were 2 other heterozygous traits (hemoglobin E and Constant Spring), parity, RBP, log ferritin, and vitamin B-12. Multiple biomarkers for anemia and iron deficiency were significantly influenced by the presence of hemoglobin disorders, hence reducing their diagnostic sensitivity. Further investigation of the unexpectedly low prevalence of IDA in Cambodian women is warranted. © 2015 American Society for Nutrition.

The Segmented Aperture Interferometric Nulling Testbed (SAINT) I: overview and air-side system description

Science.gov (United States)

Hicks, Brian A.; Lyon, Richard G.; Petrone, Peter; Ballard, Marlin; Bolcar, Matthew R.; Bolognese, Jeff; Clampin, Mark; Dogoda, Peter; Dworzanski, Daniel; Helmbrecht, Michael A.; Koca, Corina; Shiri, Ron

2016-07-01

This work presents an overview of the Segmented Aperture Interferometric Nulling Testbed (SAINT), a project that will pair an actively-controlled macro-scale segmented mirror with the Visible Nulling Coronagraph (VNC). SAINT will incorporate the VNC's demonstrated wavefront sensing and control system to refine and quantify end-to-end high-contrast starlight suppression performance. This pathfinder testbed will be used as a tool to study and refine approaches to mitigating instabilities and complex diffraction expected from future large segmented aperture telescopes.

Lyophilized bovine hemoglobin as a possible reference material for the determination of hemoglobin derivatives in human blood

NARCIS (Netherlands)

Maas, BHA; Buursma, A; Ernst, RAJ; Maas, AHJ; Zijlstra, WG

We investigated the suitability of a lyophilized bovine hemoglobin (LBH) preparation containing various fractions of oxyhemoglobin (O(2)Hb), carboxyhemoglobin (COHb), and methemoglobin (MetHb) for quality assessment in multicomponent analysis (MCA) of hemoglobin derivatives. It was demonstrated that

Lyophilized bovine hemoglobin as a possible reference material for the determination of hemoglobin derivatives in human blood

NARCIS (Netherlands)

Maas, BHA; Buursma, A; Ernst, RAJ; Maas, AHJ; Zijlstra, WG

1998-01-01

We investigated the suitability of a lyophilized bovine hemoglobin (LBH) preparation containing various fractions of oxyhemoglobin (O(2)Hb), carboxyhemoglobin (COHb), and methemoglobin (MetHb) for quality assessment in multicomponent analysis (MCA) of hemoglobin derivatives. It was demonstrated that

Public meeting of September 13, 2005 at Saint-Dizier; Reunion publique du 13 septembre 2005 a Saint-Dizier

Energy Technology Data Exchange (ETDEWEB)

NONE

2005-07-01

The law from December 30, 1991, precisely defines 3 axes of researches for the management of high level and long-lived radioactive wastes: separation/transmutation, surface storage and underground disposal. A global evaluation report about these researches is to be supplied in 2006 by the French government to the Parliament. A first synthesis of the knowledge gained after 14 years of research has led the national commission of the public debate (CNDP) to organize a national debate about the general options of management of high-level and long-lived radioactive wastes before the 2006 date line. The debate comprises 4 public hearings (September 2005: Bar-le-Duc, Saint-Dizier, Pont-du-Gard, Cherbourg), 12 round-tables (October and November 2005: Paris, Joinville, Caen, Nancy, Marseille), a synthesis meeting (December 2005, Dunkerque) and a closing meeting (January 2006, Lyon). This document is the synthesis of the debates of the second public hearing at Saint-Dizier. This meeting gathers representatives of the different actors of the nuclear industry, ministers, public authorities, non governmental organizations who argue the questions asked by peoples from the audience. The topics concern the safety aspects of waste facilities, in particular according to the studies carried out at the Bure site, the different categories of wastes and the future wastes that will be generated by the dismantling of nuclear facilities etc. (J.S.)

Life and death at precolumbian Lavoutte, Saint Lucia, Lesser Antilles

NARCIS (Netherlands)

Hofman, C.L.; Hoogland, M.L.P.; Mickleburgh, H.L.; Laffoon, J.E.; Weston, D.A.; Field, M.H.

2012-01-01

The Caribbean archaeological record requires immediate attention and protection. Development and natural forces have impacted archaeological sites, destroying or severely damaging them. The precolumbian site of Lavoutte, located in northern Saint Lucia, has been known as a major Late Ceramic Age

What Are Robespierre and Telemachus Doing in Saint-Domingue?

DEFF Research Database (Denmark)

Kjærgård, Jonas Ross

2018-01-01

Charles Pigault-Lebrun's play Le blanc et le noir (1795) is one of only a handful of French revolutionary plays to stage the Saint-Domingue slave rebellion, a rebellion today better known as the Haitian Revolution (1791–1804). I argue that Pigault-Lebrun uses early melodramatic generic traits to ...

The Way of Saint James and the Xacobeo 2010 in the tourism websites of the Spanish autonomous communities

Directory of Open Access Journals (Sweden)

María-Dolores Fernández-Poyatos, Ph.D.

2012-01-01

Full Text Available This article analyses the tourism websites of Spanish autonomous communities crossed by the Way of Saint James. Considering that the Way of Saint James and the Xacobeo 2010 are important touristic opportunities for these autonomous regions, the main objective of this investigation is to evaluate the information about these touristic places in their tourism websites. The analysis focused on two aspects: firstly, establishing whether the autonomous communities have used the Way of Saint James and the Xacobeo 2010 to promote themselves, and secondly, evaluating the quality of the websites. The study included a literature review, definition of concepts and criteria to measure the quality of websites, and the analysis of the content and design of the selected tourism websites. The results show that the Galician website is the most complete in terms of information about the Way of Saint James and the Xacobeo 2010. The information about the Way of Saint James and the Xacobeo 2010 in the websites of the other communities is vague and differs from one website to another. Due to the touristic significance of the Xacobeo 2010 and the importance of Internet as a medium of communication, the research group plans to conduct a new analysis of the communities’ institutional websites in 2011.

The Slavonic Translation of the Life of Saint Euthymius the Great: Observations on Language and Authorship

Directory of Open Access Journals (Sweden)

Dmitriy Evgenyevich Afinogenov

2015-10-01

Full Text Available The Slavonic translation of the Life of Saint Euthymius the Great (BHG 647 by Cyril of Scythopolis, attested at the earliest manuscript of the late 14th c., was probably made by a person originating from South-East Rus in the 11th c., as it displays some grammatical, lexical, and phonetic features peculiar for this area and time, and visible, in particular, in Ipatiev Chronicle and the Tale of Igor’s Campaign. It may have formed a part of the collection comprising several writings by Cyril, including the Life of Saint Sabas and the Praise of Euthymius and Sabas (lost in the Greek original. This collection was later incorporated into the Great Lectionary Menologion of Metropolitan Makarij. The details added by the translator to the description of certain realia pertaining to the Monastery of Saint Euthymius in Palestine suggest a personal knowledge of that monastic complex (the Laura and the coenobium. The proposed author of the translation is Saint Anthony of the Cave Monastery in Kiev († 1073, who could perfectly well have visited the Holy Land during his stay on Mount Athos prior to 1051.

Description of Three New α Variants and Four New β Variants: Hb Montluel [α110(G17)Ala → Val; HBA1: c.332C > T], Hb Cap d'Agde [α131(H14)Ser → Cys; HBA2: c.395C > G] and Hb Corsica [α100(G7)Leu → Pro; HBA1: 302T > C]; Hb Nîmes [β104(G6)Arg → Gly; HBB: c.313A > G], Hb Saint Marcellin [β112(G14)Cys → Gly; HBB: c.337T > G], Hb Saint Chamond [β80(EF4)Asn → 0; HBB: c.241_243delAAC] and Hb Dompierre [β29(B11)Gly → Arg; HBB: c.88G > C].

Science.gov (United States)

Renoux, Céline; Feray, Cécile; Joly, Philippe; Lacan, Philippe; Francina, Alain

2015-01-01

We present here seven new hemoglobin (Hb) variants identified during routine Hb analysis. All of them are caused by a missense mutation except Hb Saint Chamond, which results from an in-frame deletion of the asparagine residue at β80. All these variants are clinically silent in the heterozygous state but two of them (Hb Cap d'Agde and Hb Dompierre) may be unstable, whereas Hb Nîmes could present a very slightly elevated oxygen affinity. These data are to be confirmed by appropriate biochemical tests.

L’église Saint-Michel de Lille : chronologie d’un chantier

Directory of Open Access Journals (Sweden)

Catherine Guillot

2012-04-01

Full Text Available Après le démantèlement des fortifications de Lille en 1858, un nouveau quartier est aménagé au sud de la ville, dont un des équipements importants est l’église Saint-Michel, construite entre 1868 et 1874 par l’architecte Alfred Coisel. Très bien documenté, son chantier dévoile un mobilier et un décor exemplaire par son homogénéité et par la place accordée à la peinture murale. Cette étude met en évidence, outre le rôle du peintre Alphonse Colas, des artistes méconnus comme Albéric Duyver et Marie Carin. L’iconographie, qui révèle un des plus importants cycles consacrés à l’archange saint Michel, est également extrêmement caractéristique de son temps.After the demolition of the fortifications at Lille, in 1858, a new quarter developed to the south of the city. One of the important features of this new neighbourhood was the Saint-Michel church, built between 1868 and 1874 to the designs of the architect Alfred Coisel. The building of this church is exceptionally well documented and reveals church furnishings and decoration of an exemplary nature in terms of homogeneity and the special place given over to mural paintings. This study underlines the role of the painter Alphonse Colas, but also the contribution of other, less well known artists such as Albéric Duyver and Marie Carin. The iconographical theme is one of the most important cycles of its time devoted to the archangel Saint Michel, and is highly characteristic of the period.

Egg deposition by lithophilic-spawning fishes in the Detroit and Saint Clair Rivers, 2005–14

Science.gov (United States)

Prichard, Carson G.; Craig, Jaquelyn M.; Roseman, Edward F.; Fischer, Jason L.; Manny, Bruce A.; Kennedy, Gregory W.

2017-03-14

A long-term, multiseason, fish egg sampling program conducted annually on the Detroit (2005–14) and Saint Clair (2010–14) Rivers was summarized to identify where productive fish spawning habitat currently exists. Egg mats were placed on the river bottom during the spring and fall at historic spawning areas and candidate fish spawning habitat restoration sites throughout both rivers. Widespread evidence was found of lithophilic spawning by numerous native fish species, including walleye (Sander vitreus), lake whitefish (Coregonus clupeaformis), lake sturgeon (Acipenser fulvescens), suckers (Catostomidae spp.), and trout-perch (Percopsis omiscomaycus). Walleye, lake whitefish, and suckers spp. spawned in nearly every region of each river in all years on both reef and nonreef substrates. Lake sturgeon eggs were collected almost exclusively over constructed reefs. Catch-per-unit effort of walleye, lake whitefish, and sucker eggs was much greater in the Detroit River than in the Saint Clair River, while Saint Clair River sites supported the greatest collections of lake sturgeon eggs. Collections during this study of lake sturgeon eggs on man-made spawning reefs suggest that artificial reefs may be an effective tool for restoring fish populations in the Detroit and Saint Clair Rivers; however, the quick response of lake sturgeon to spawn on newly constructed reefs and the fact that walleye, lake whitefish, and sucker eggs were often collected over substrate with little interstitial space to protect eggs from siltation and predators suggests that lack of suitable spawning habitat may continue to limit reproduction of lithophilic-spawning fish species in the Saint Clair-Detroit River System.

Calculation of Void in the Fort Saint Vrain Material

Energy Technology Data Exchange (ETDEWEB)

Potter, David Charles [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Taylor, Craig Michael [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Coons, James Elmer [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

2017-05-11

The percent void of the Fort Saint Vrain (FSV) material is estimated to be 21.1% based on the volume of the gap at the top of the drums, the volume of the coolant channels in the FSV fuel element, and the volume of the fuel handling channel in the FSV fuel element.

Postoperative hemoglobin level in patients with femoral neck fracture.

Science.gov (United States)

Nagra, Navraj S; Van Popta, Dmitri; Whiteside, Sigrid; Holt, Edward M

2016-01-01

The aim of this study was to analyze the changes of hemoglobin levels in patients undergoing fixation for femoral neck fracture. Peroperative hemoglobin levels of patients who underwent either dynamic hip screw (DHS) fixation (n=74; mean age: 80 years) or hip hemiarthroplasty (n=104; mean age: 84 years) for femoral neck fracture was monitored. There was a statistically and clinically significant mean drop of 31.1 g/L between the preoperative (D0) and postoperative Day 5 Hb levels (pmeasurement, DHS patients had lower hemoglobin values over hemiarthroplasty patients (p=0.046). The decrease in hemoglobin in the first 24-hour postoperative period (D0 to Day 1) is an underestimation of the ultimate lowest value in hemoglobin found at Day 2. Relying on the Day 1 hemoglobin level could be detrimental to patient care. We propose a method of predicting patients likely to be transfused and recommend a protocol for patients undergoing femoral neck fracture surgery to standardize postoperative hemoglobin monitoring.

IAEA Operational Safety Team Reviews Saint-Alban Nuclear Power Plant, France

International Nuclear Information System (INIS)

2010-01-01

Full text: An international team of nuclear installation safety experts, led by the International Atomic Energy Agency (IAEA), has reviewed safety practices at France's Saint-Alban Nuclear Power Plant (NPP) and has highlighted a set of strong practices as well as a series of recommendations to reinforce them. The IAEA assembled the team at the request of the Government of France to conduct an Operational Safety Review (OSART) of the Saint-Alban NPP. Under the leadership of the IAEA's Division of Nuclear Installation Safety in Vienna, the OSART team performed an in-depth operational safety review from 20 September to 6 October 2010. The team was made up of experts from Belgium, Canada, the Czech Republic, Germany, Lithuania, the Netherlands, Slovakia, Sweden and the USA. An OSART mission is designed to review programmes and activities essential to operational safety. It is not a regulatory inspection, nor is it a design review or a substitute for an exhaustive assessment of the plant's overall safety status. The team at Saint-Alban conducted an in-depth review of the aspects essential to the safe operation of the NPP, which largely are under the control of the site management. The conclusions of the review are based on the IAEA's Safety Standards and proven good international practices. The review covered the areas of Management, Organization and Administration; Training and Qualification; Operations; Maintenance; Technical Support; Operating Experience; Radiation Protection; Chemistry; and Emergency Planning and Preparedness. The OSART team has identified good plant practices, which will be shared with the rest of the nuclear industry for consideration of their application. Examples include: A safety guideline for outages; The use of remote video surveillance of fuel inspection and handling activities; A motivational tool for plant staff regarding the benefits of operating experience and associated corrective actions; and Use of a sophisticated key control system

Saint John lateral pipeline project: comprehensive study report

International Nuclear Information System (INIS)

1999-02-01

A descripion is given of the results of an environmental impact assessment of the construction of a natural gas pipeline that will bring gas from Maritimes and Northeast Pipeline Management Ltd.'s (M and NP) main transmission pipeline to clients in Lake Utopia and Saint John, NB. It will be 110 km long, and the Saint John lateral will join the mainline approximately 2 km south of Big Kedron Lake, NB. Various institutional, industrial, commercial and residential clients will be able to access the pipeline in the future, and it is intended to be capable of future expansion for markets along its route and for markets that become economically feasible in the future. A matrix was developed that relates environmental resources to Environmental Components of Concern (ECCs), and to the rationale for exclusion/inclusion of the ECC as a Valued Environmental Component (VECs). The positive benefits of the pipeline to clients in the Saint John area include: reduced dependence on foreign oil, fuel switching and price competition, lower air emission pollution, increased energy efficiency, and a source of energy that is secure and reliable. VECs were selected based on: concerns of various stakeholders including the public, community groups, scientific parties, Aboriginal groups, government officials and agencies, relevant regulations; relevant literature; and past evaluation experiences, including future developments of the proposed study area. Further selection of the VECs required an examination of the issues picked out via scoping to determine the ways in which the overall project could affect the ECCs, and included construction, operation, decommissioning/abandonment, malfunctions and accidents of the pipeline. The phases of the project as they impacted residually and cumulatively on the VECs were evaluated, and impacts on Valued Socio-Economic Components (VSCs) were assessed also. The mitigation efforts taken will minimize the potential effects of the project on VECs and VSCs

Hemoglobin levels in normal Filipino pregnant women.

Science.gov (United States)

Kuizon, M D; Natera, M G; Ancheta, L P; Platon, T P; Reyes, G D; Macapinlac, M P

1981-09-01

The hemoglobin concentrations during pregnancy in Filipinos belonging to the upper income group, who were prescribed 105 mg elemental iron daily, and who had acceptable levels of transferrin saturation, were examined in an attempt to define normal levels. The hemoglobin concentrations for each trimester followed a Gaussian distribution. The hemoglobin values equal to the mean minus one standard deviation were 11.4 gm/dl for the first trimester and 10.4 gm/dl for the second and third trimesters. Using these values as the lower limits of normal, in one group of pregnant women the prevalence of anemia during the last two trimesters was found lower than that obtained when WHO levels for normal were used. Groups of women with hemoglobin of 10.4 to 10.9 gm/dl (classified anemic by WHO criteria but normal in the present study) and those with 11.0 gm/dl and above could not be distinguished on the basis of their serum ferritin levels nor on the degree of decrease in their hemoglobin concentration during pregnancy. Many subjects in both groups, however, had serum ferritin levels less than 12 ng/ml which indicate poor iron stores. It might be desirable in future studies to determine the hemoglobin cut-off point that will delineate subjects who are both non-anemic and adequate in iron stores using serum ferritin levels as criterion for the latter.

A School-Based Enterprise: The Saint Pauls, North Carolina Experience.

Science.gov (United States)

Paquin, Thomas F.

1991-01-01

The superintendent of Saint Pauls Schools describes how educators, community leaders, businesspersons, and high school students worked together to develop the Way Off Broadway Deli, a successful school-based enterprise providing experiential education in entrepreneurship and small business management, as well as student employment and local…

Ecole d’été de probabilités de Saint-Flour XXVIII

CERN Document Server

2000-01-01

This volume contains lectures given at the Saint-Flour Summer School of Probability Theory during 17th Aug. - 3rd Sept. 1998. The contents of the three courses are the following: - Continuous martingales on differential manifolds. - Topics in non-parametric statistics. - Free probability theory. The reader is expected to have a graduate level in probability theory and statistics. This book is of interest to PhD students in probability and statistics or operators theory as well as for researchers in all these fields. The series of lecture notes from the Saint-Flour Probability Summer School can be considered as an encyclopedia of probability theory and related fields.

The “Enemy” of the Saint in the Vita Fulgentii

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Isabella D'Auria

2015-06-01

Full Text Available This paper aims to analyze the traits that characterize the “enemy” of the saint in the Vita Fulgentii. The work tells in twenty-nine chapters the life of the bishop of Ruspe Fulgentius, troubled by exile and persecutions both in the monastic phase and in the episcopal one, in the context of the vandalic invasion in Africa between 5th and 6th century. The “enemy”, that identifies, in the most cases, with the invader of arian faith, presents a stereotyped nature, summarizing the tipical characteristics of barbarian and the ones specific to the heterodox. In order to achieve the goal of the reader’s moral edification, the text presents antithetical models, the saint ant his opposer, and all the account revolves around this dialectic; so it is worthy of interest to pick out the elements that define, throught a relationship of opposition, the collective identities, that in this text are represented by Catholics invaded and by barbarian and heretic invaders.

Heterozygote Hemoglobin G-Coushatta as the Cause of a Falsely Decreased Hemoglobin A1C in an Ion-Exchange HPLC Method

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Kurtoğlu Ayşegül Uğur

2017-09-01

Full Text Available Glycated hemoglobin (HbA1c is used for the assessment of glycemic control in patients with diabetes. The presence of genetic variants of hemoglobin can profoundly affect the accuracy of HbA1c measurement. Here, we report two cases of Hemoglobin G-Coushatta (HBB:c.68A>C variant that interferes in the measurement of HbA1c by a cation-exchange HPLC (CE-HPLC method. HbA1c was measured by a CE-HPLC method in a Tosoh HLC-723 G7 instrument. The HbA1c levels were 2.9% and 4%. These results alerted us to a possible presence of hemoglobinopathy. In the hemoglobin variant analysis, HbA2 levels were detected as 78.3% and 40.7% by HPLC using the short program for the Biorad Variant II. HbA1c levels were measured by an immunoturbidimetric assay in a Siemens Dimension instrument. HbA1c levels were reported as 5.5% and 5.3%. DNA mutation analysis was performed to detect the abnormal hemoglobin variant. Presence of Hemoglobin G-Coushatta variant was detected in the patients. The Hb G-Coushatta variants have an impact on the determination of glycated hemoglobin levels using CEHPLC resulting in a false low value. Therefore, it is necessary to use another measurement method.

Bunker culturel : la régénération du patrimoine militaire urbain à Saint-Nazaire

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Renzo Lecardane

2011-06-01

Full Text Available Au cours des deux dernières décennies Saint-Nazaire a relancé son développement sans se référer nécessairement aux modèles des métropoles européennes. Concentrées autour de la zone portuaire, près de la base sous-marine allemande, les nouvelles opérations urbaines ont contribué à définir un important projet touristique et culturel. La spécificité symbolique et la valorisation du patrimoine militaire ont permis à Saint-Nazaire de montrer que l’identité d’une ville pouvait activer sa croissance.Over the last two decades, the port city of Saint-Nazaire has encouraged urban redevelopment without necessarily following the models suggested by other European cities. An important cultural and tourist centre has been developed around the port zone, close to the German submarine base dating from the Second World War. The symbolic specificity and the interpretation of this military heritage have shown, at Saint-Nazaire, how a town's special identity can foster growth.

Individualized anemia management reduces hemoglobin variability in hemodialysis patients.

Science.gov (United States)

Gaweda, Adam E; Aronoff, George R; Jacobs, Alfred A; Rai, Shesh N; Brier, Michael E

2014-01-01

One-size-fits-all protocol-based approaches to anemia management with erythropoiesis-stimulating agents (ESAs) may result in undesired patterns of hemoglobin variability. In this single-center, double-blind, randomized controlled trial, we tested the hypothesis that individualized dosing of ESA improves hemoglobin variability over a standard population-based approach. We enrolled 62 hemodialysis patients and followed them over a 12-month period. Patients were randomly assigned to receive ESA doses guided by the Smart Anemia Manager algorithm (treatment) or by a standard protocol (control). Dose recommendations, performed on a monthly basis, were validated by an expert physician anemia manager. The primary outcome was the percentage of hemoglobin concentrations between 10 and 12 g/dl over the follow-up period. A total of 258 of 356 (72.5%) hemoglobin concentrations were between 10 and 12 g/dl in the treatment group, compared with 208 of 336 (61.9%) in the control group; 42 (11.8%) hemoglobin concentrations were hemoglobin concentrations were >12 g/dl in the treatment group compared with 46 (13.4%) in the control group. The median ESA dosage per patient was 2000 IU/wk in both groups. Five participants received 6 transfusions (21 U) in the treatment group, compared with 8 participants and 13 transfusions (31 U) in the control group. These results suggest that individualized ESA dosing decreases total hemoglobin variability compared with a population protocol-based approach. As hemoglobin levels are declining in hemodialysis patients, decreasing hemoglobin variability may help reduce the risk of transfusions in this population.

The interaction of 2,3-diphosphoglycerate with various human hemoglobins

Science.gov (United States)

Bunn, H. Franklin; Briehl, Robin W.

1970-01-01

Oxygen equilibria were measured on a number of human hemoglobins, which had been “stripped” of organic phosphates and isolated by column chromatography. In the presence of 2 × 10-4 M 2,3-diphosphoglycerate (2,3-DPG), the P50 of hemoglobins A, A2, S, and C increased about twofold, signifying a substantial and equal decrease in oxygen affinity. Furthermore, hemoglobins Chesapeake and MMilwaukee-1 which have intrinsically high and low oxygen affinities, respectively, also showed a twofold increase in P50 in the presence of 2 × 10-4 M 2,3-DPG. In comparison to these, hemoglobins AIC and F were less reactive with 2,3-DPG while hemoglobin FI showed virtually no reactivity. The N-terminal amino of each β-chain of hemoglobin AIC is linked to a hexose. In hemoglobin FI the N-terminal amino of each γ-chain is acetylated. These results suggest that the N-terminal amino groups of the non-α-chains are involved in the binding of 2,3-DPG to hemoglobin. PMID:5422014

The eleventh CRL-Salmonella workshop, 9 May 2006, Saint Malo, France

NARCIS (Netherlands)

Mooijman KA; MGB

2006-01-01

De elfde workshop georganiseerd door het Communautair Referentie Laboratorium voor Salmonella (CRL-Salmonella) werd gehouden op 9 mei 2006 in Saint Malo, Frankrijk. Deelnemers waren vertegenwoordigers van de nationale Referentie Laboratoria voor Salmonella (NRLs-Salmonella) van de lidstaten van de

Porphyromonas endodontalis binds, reduces and grows on human hemoglobin.

Science.gov (United States)

Zerr, M; Drake, D; Johnson, W; Cox, C D

2001-08-01

Porphyromonas endodontalis is a black-pigmented, obligate anaerobic rod-shaped bacterium implicated as playing a major role in endodontic infections. We have previously shown that P. endodontalis requires the porphyrin nucleus, preferably supplied as hemoglobin, as a growth supplement. The bacteria also actively transport free iron, although this activity does not support growth in the absence of a porphyrin source. The purpose of this study was to further investigate the binding and subsequent utilization of human hemoglobin by P. endodontalis. P. endodontalis binds hemoglobin and reduces the Fe(III) porphyrin, resulting in a steady accumulation of ferrous hemoglobin. Reduction of methemoglobin was similar to the extracellular reduction of nitrobluetetrazolium in the presence of oxidizable substrate. Turbidimetric and viable cell determinations showed that P. endodontalis grew when supplied only hemoglobin. Therefore, we conclude that hemoglobin appears to serve as a sole carbon and nitrogen source, and that these bacteria reduce extracellular compounds at the expense of oxidized substrates.

Post-transfusion hemoglobin values and patient blood management

DEFF Research Database (Denmark)

Moerman, Jan; Vermeulen, Edith; Van Mullem, Mia

2018-01-01

Objectives: The objective of this retrospective study was to evaluate the added value of communicating post-transfusion hemoglobin values to clinicians as a strategy to improve RBC utilization in a 500-bed hospital. Methods: The total number of RBC transfusions, the mean number of RBC units...... transfused per patient, the mean pre- and post-transfusion hemoglobin values, the ratio of patients transfused and the ratio of patients with a post-transfusion hemoglobin > 10.5 g/dL were calculated per service and per department for six months. The data were reported to each service and compared...... with the data of the department as peer group. The impact of this communication strategy was evaluated in the following six months. Results: In the six months pre-intervention, the mean post-transfusion hemoglobin value was 9.2 g/dL. Post-transfusion hemoglobin was > 10.5 g/dL in 13.4% of patients (112...

Hemoglobin affinity in Andean rodents

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HRVOJ OSTOJIC

2002-01-01

Full Text Available Blood hemoglobin oxygen affinity (P50 was measured in three Andean species and in the laboratory rat (control, all raised near sea level. Chinchilla lanigera (Molina, 1792 has an altitudinal habitat range from low Andean slopes up to 3000 m., while Chinchilla brevicaudata (Waterhouse, 1848 has an altitudinal range from 3000 to 5000 m. The laboratory type guinea pig, wild type guinea pig (Cavia porcellus, (Waterhouse, 1748, and laboratory rat (Rattus norvegicus were also raised at sea level. The Andean species had high hemoglobin oxygen affinities (low P50 compared with the rat. Chinchilla brevicaudata had a higher affinity than Chinchilla lanigera. The wild type guinea pig had a higher affinity than the laboratory type. As has been shown in other species, this is another example of an inverse correlation between the altitude level and the P50 values. This is the first hemoglobin oxygen affinity study in Chinchilla brevicaudata.

Un solveur HLLT pour les equations de Saint-Venant et traitement ...

African Journals Online (AJOL)

Un solveur HLLT pour les equations de Saint-Venant et traitement des ... Enfin, des applications numériques sur des cas tests sont présentées. ... the system of partial-differential equations is completed by a trivial equation for the bathymetry.

Un solveur HLLTpour les equations de Saint-vVenant et traitement ...

African Journals Online (AJOL)

Un solveur HLLTpour les equations de Saint-vVenant et traitement des ... Enfin, des applications numériques sur des cas tests sont présentées. ... the system of partial-differential equations is completed by a trivial equation for the bathymetry.

Developing Semi-Analytical solutions for Saint-Venant Equations in the Uniform Flow Region

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M.M. Heidari

2016-09-01

Full Text Available Introduction: Unsteady flow in irrigation systems is the result of operations in response to changes in water demand that affect the hydraulic performance networks. The increased hydraulic performance needed to recognize unsteady flow and quantify the factors affecting it. Unsteady flow in open channels is governed by the fully dynamic Saint Venant equation, which express the principles of conservation of mass and momentum. Unsteady flow in open channels can be classified into two types: routing and operation-type problems. In the routing problems, The Saint Venant equations are solved to get the discharge and water level in the time series. Also, they are used in the operation problem to compute the inflow at the upstream section of the channel according to the prescribed downstream flow hydrographs. The Saint Venant equation has no analytical solution and in the majority cases of such methods use numerical integration of continuity and momentum equations, and are characterized by complicated numerical procedures that are not always convenient for carrying out practical engineering calculations. Therefore, approximate methods deserve attention since they would allow the solution of dynamic problems in analytical form with enough exactness. There are effective methods for automatic controller synthesis in control theory that provide the required performance optimization. It is therefore important to get simplified models of irrigation canals for control design. It would be even more interesting to have linear models that explicitly depend on physical parameters. Such models would allow one to, handle the dynamics of the system with fewer parameters, understand the impact of physical parameters on the dynamics, and facilitate the development a systematic design method. Many analytical models have been proposed in the literature, Most of them have been obtained in the frequency domain by applying Laplace transform to linearized Saint

Biphasic oxidation of oxy-hemoglobin in bloodstains

NARCIS (Netherlands)

Bremmer, Rolf H.; de Bruin, Daniel M.; de Joode, Maarten; Buma, Wybren Jan; van Leeuwen, Ton G.; Aalders, Maurice C. G.

2011-01-01

In forensic science, age determination of bloodstains can be crucial in reconstructing crimes. Upon exiting the body, bloodstains transit from bright red to dark brown, which is attributed to oxidation of oxy-hemoglobin (HbO(2)) to met-hemoglobin (met-Hb) and hemichrome (HC). The fractions of

Biphasic Oxidation of Oxy-Hemoglobin in Bloodstains

NARCIS (Netherlands)

Bremmer, R.H.; de Bruin, D.M.; de Joode, M.; Buma, W.J.; van Leeuwen, T.G.; Aalders, M.C.G.

2011-01-01

Background In forensic science, age determination of bloodstains can be crucial in reconstructing crimes. Upon exiting the body, bloodstains transit from bright red to dark brown, which is attributed to oxidation of oxy-hemoglobin (HbO2) to met-hemoglobin (met-Hb) and hemichrome (HC). The fractions

Kadar Hemoglobin dan Kecerdasan Intelektual Anak

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Yuni Kusmiyati

2013-10-01

Full Text Available Kualitas sumber daya manusia dipengaruhi oleh inteligensi anak. Skor kecerdasan intelektual yang tidak menetap pada usia tertentu dapat berubah karena faktor genetik, gizi, dan lingkungan. Tujuan penelitian ini adalah mengetahui hubungan kadar hemoglobin dengan kecerdasan intelektual anak. Penelitian observasional dengan desain potong lintang ini dilakukan pada populasi siswa kelas VI Sekolah Dasar Negeri Giwangan Yogyakarta, tahun 2013. Penarikan sampel dilakukan dengan metode simple random sampling terhadap 37 sampel siswa. Instrumen untuk mengukur kecerdasan intelektual dengan Cultural Fair Intelligence Quotient Test yang dirancang untuk meminimalkan pengaruh kultural dengan memperhatikan prosedur evaluasi, instruksi, konten isi, dan respons peserta. Tes dilakukan oleh Biro Psikologi Universitas Ahmad Dahlan Yogyakarta, kadar hemoglobin diukur menggunakan Portable Hemoglobin Digital Analyzer Easy Touch secara digital.Variabel luar indeks massa tubuh diukur langsung menggunakan parameter tinggi badan dan berat badan. Analisis menggunakan uji regresi linier. Hasil penelitian menunjukkan indeks massa tubuh tidak berhubungan dengan kecerdasan intelektual (nilai p = 0,052. Anemia berhubungan cukup dengan kecerdasan anak (r = 0,491 dan berpola positif, semakin tinggi kadar hemoglobin semakin tinggi kecerdasan intelektual anak. Nilai koefisien determinasi 0,241 menerangkan bahwa 24,1% variasi anemia cukup baik untuk menjelaskan variabel kecerdasan intelektual. Ada hubungan antara kadar hemoglobin dengan kecerdasan intelektual (nilai p = 0,002. Quality of human resources is influenced by the child’s intelligent. Intelligence Quotient (IQ score will not settle at a certain age and can change due to genetic factors, nutrition, and the environment. The objective is known relationship of anemia with IQ to child. Method of observational study with cross sectional design. Population are students of class VI elementary school of Giwangan Yogyakarta in

Receptor targeting of hemoglobin mediated by the haptoglobins

DEFF Research Database (Denmark)

Nielsen, Marianne Jensby; Moestrup, Søren Kragh

2009-01-01

Haptoglobin, the haptoglobin-hemoglobin receptor CD163, and the heme oxygenase-1 are proteins with a well-established function in the clearance and metabolism of "free" hemoglobin released during intravascular hemolysis. This scavenging system counteracts the potentially harmful oxidative and NO......-scavenging effects associated with "free" hemoglobin, and, furthermore, elicits an anti-inflammatory response. In the late primate evolution, haptoglobin variants with distinct functions have arisen, including haptoglobin polymers and the haptoglobin-related protein. The latter associates with a subspecies of high......-density lipoprotein (HDL) particles playing a crucial role in the innate immunity against certain trypanosome parasites. Recent studies have elucidated this fairly sophisticated immune defense mechanism that takes advantage of a trypanosomal haptoglobin-hemoglobin receptor evolved to supply the parasite with heme...

Cell volume regulation in hemoglobin CC and AA erythrocytes

International Nuclear Information System (INIS)

Berkowitz, L.R.; Orringer, E.P.

1987-01-01

Swelling hemoglobin CC erythrocytes stimulates a ouabain-insensitive K flux that restores original cell volume. Studies were performed with the K analog, 86 Rb. This volume regulatory pathway was characterized for its anion dependence, sensitivity to loop diuretics, and requirement for Na. The swelling-induced K flux was eliminated if intracellular chloride was replaced by nitrate and both swelling-activated K influx and efflux were partially inhibited by 1 mM furosemide or bumetanide. K influx in swollen hemoglobin CC cells was not diminished when Na in the incubation medium was replaced with choline, indicating Na independence of the swelling-induced flux. Identical experiments with hemoglobin AA cells also demonstrated a swelling-induced increase in K flux, but the magnitude and duration of this increase were considerably less than that seen with hemoglobin CC cells. The increased K flux in hemoglobin AA cells was likewise sensitive to anion replacement and to loop diuretics and did not require the presence of Na. These data indicate that a volume-activated K pathway with similar transport characteristics exists in both hemoglobin CC and AA red cells

Hemoglobin and heme scavenger receptors

DEFF Research Database (Denmark)

Nielsen, Marianne Jensby; Møller, Holger Jon; Moestrup, Søren Kragh

2010-01-01

Heme, the functional group of hemoglobin, myoglobin, and other hemoproteins, is a highly toxic substance when it appears in the extracellular milieu. To circumvent potential harmful effects of heme from hemoproteins released during physiological or pathological cell damage (such as hemolysis...... and rhabdomyolysis), specific high capacity scavenging systems have evolved in the mammalian organism. Two major systems, which essentially function in a similar way by means of a circulating latent plasma carrier protein that upon ligand binding is recognized by a receptor, are represented by a) the hemoglobin...

Sexual dimorphism in the mandible of the armadillo Chaetophractus villosus (Desmarest, 1804 (Dasypodidae from northern Patagonia, Argentina Dimorfismo sexual da mandíbula do tatu Chaetophractus villosus (Desmarest, 1804 (Dasypodidae do norte da Patagônia Argentina

Directory of Open Access Journals (Sweden)

SM. Squarcia

2009-05-01

Full Text Available The aim of this study was to study the sexual dimorphism in adult Chaetophractus villosus (Desmarest, 1804, from northern Patagonia, Argentina. Eight mandibular traits were measured in 37 males and 34 females. Univariate and multivariate morphometric analysis were applied to the data set. Results showed that C. villosus was sexually dimorphic, with higher absolute values corresponding to females. The total length of the mandible was the most important variable to discriminate sexes, followed by the height at the level of the last tooth and body length. The percentages of sex discrimination were high, as they were when a new sample (17 males, 13 females was tested. Females have larger mandibles than males independently of their larger cranial size. They also showed a higher degree of correlation between variables, suggesting a more stable shape for the mandible than in males.O presente estudo examinou o dimorfismo sexual dos adultos de Chaetophractus villosus (Desmarest, 1804 do norte da Patagônia Argentina. Oito parâmetros mandibulares foram medidos em 37 machos e 34 fêmeas. Análises morfométricas univariada e multivariada foram aplicadas à série de dados. Os resultados mostraram que C. villosus apresenta dimorfismo sexual, com todos os valores absolutos mais elevados nas fêmeas. O comprimento total da mandíbula foi a variável mais importante para discriminar os sexos, seguidos pela altura no nível dos últimos dentes e pelo comprimento do corpo da mandíbula. As porcentagens de discriminação dos sexos foram elevadas, quando uma amostra nova (17 machos, 13 fêmeas foi testada. As fêmeas têm as mandíbulas maiores que os machos, independentemente de seu tamanho cranial maior. Mostraram também um grau mais elevado de correlação entre variáveis, sugerindo uma forma mais estável para a mandíbula que nos machos.

21 CFR 864.7455 - Fetal hemoglobin assay.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Fetal hemoglobin assay. 864.7455 Section 864.7455 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Kits and Packages § 864.7455 Fetal hemoglobin...

Síntese e caracterização de um novo composto obtido pela reação entre hidreto de trifenilestanho e ácido (±-mandélico e avaliação de seu potencial biocida sobre o fungo Fusarium oxysporum f. sp. cubense Synthesis, characterization and evaluation of the biocide effect on the fungus Fusarium oxysporum f. sp. cubense of a new compound obtained by reaction of triphenyltin hydride and (±-mandelic acid

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Roberto Santos Barbiéri

2006-06-01

Full Text Available O presente artigo refere-se à síntese e caracterização de um novo composto organoestânico, pela reação de ácido (±-mandélico e hidreto de trifenilestanho, em meio de acetonitrila e sob refluxo, [(C6H52SnMand 2] {Mand = C6H5CH(OHCOO], identificado por análise elementar de carbono e hidrogênio, espectroscopia no infravermelho e espectrometria de massa de alta resolução, para o qual foi proposta estrutura octaédrica com o grupo fenila em posição trans. Verificou-se que o composto apresenta ação biocida sobre o fungo Fusarium oxysporum f. sp. cubense, sendo mais efetivo que o ácido (±-mandélico livre. No entanto, a atividade biocida do composto foi menos intensa que a observada para cloreto de estanho hidratado, acetato de trifenilestanho e hidreto de trifenilestanho, empregados para fins de comparação. Nos testes de germinação de conídios e microconídios do mesmo fungo, na presença de [(C6H52SnMand 2], os índices de germinação ficaram abaixo de 11%.The present paper refers to the synthesis and characterization of a new organotin compound that was obtained by reaction of (±-mandelic acid with triphenyltin hydride in acetonitrile medium under reflux. According to hydrogen and carbon elemental analysis, infrared spectroscopy and high resolution mass spectrometry the formula of such compounds is (C6H52SnMand 2 {Mand = C6H5CH(OHCOO}. An octahedral complex, with the phenyl groups in trans position was proposed for its structure. It was observed that this compound was active against the fungus Fusarium oxysporum f. sp. cubense. The biocide effect was more intense than the one observed for(±-mandelic acid. However, it was less efficient than tin chloride hydrate, triphenyltin acetate and triphenyltin hydride. In germination assays with conides and microconides of the same fungus in the presence of [(C6H52SnMand 2], the germination rates were below 11%.

AN ORTHODOX THINKER AND A ROMANIAN HEART: THE SAINT HIERARCH ANTHIM

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Henrieta Anişoara ŞERBAN

2016-10-01

Full Text Available 2016 is an anniversary year, dedicated to the Saint Hierarch Anthim, a multi-faced personality of Georgian origin, but with a Romanian accomplished life. He was a true Orthodox believer, a Hierarch of our Orthodox Church in Wallachia and a deep thinker, who lived through the teachings of the faith. At the same time, he was a good manager and a philanthropist, a scholar, a polyglot, a calligrapher, a typographer, a Church architect, an orator turned writer, a painter and a sculptor. His great homiletic work entitled Didahiile sends to Didache, the oldest post-Bible Christian text, famous at Constantinople, known also as The Teachings of the Twelve Apostles (The Teachings of the Lord to the Gentiles (or Nations by the Twelve Apostles. The study approaches and develops these dimensions of the personality and of the thought of the Saint Hierarch Anthim, in order to emphasize both his life and his work as an esteemed symbol of the Orthodox faith.

Genomic organization and evolution of the Atlantic salmon hemoglobin repertoire

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Phillips Ruth B

2010-10-01

Full Text Available Abstract Background The genomes of salmonids are considered pseudo-tetraploid undergoing reversion to a stable diploid state. Given the genome duplication and extensive biological data available for salmonids, they are excellent model organisms for studying comparative genomics, evolutionary processes, fates of duplicated genes and the genetic and physiological processes associated with complex behavioral phenotypes. The evolution of the tetrapod hemoglobin genes is well studied; however, little is known about the genomic organization and evolution of teleost hemoglobin genes, particularly those of salmonids. The Atlantic salmon serves as a representative salmonid species for genomics studies. Given the well documented role of hemoglobin in adaptation to varied environmental conditions as well as its use as a model protein for evolutionary analyses, an understanding of the genomic structure and organization of the Atlantic salmon α and β hemoglobin genes is of great interest. Results We identified four bacterial artificial chromosomes (BACs comprising two hemoglobin gene clusters spanning the entire α and β hemoglobin gene repertoire of the Atlantic salmon genome. Their chromosomal locations were established using fluorescence in situ hybridization (FISH analysis and linkage mapping, demonstrating that the two clusters are located on separate chromosomes. The BACs were sequenced and assembled into scaffolds, which were annotated for putatively functional and pseudogenized hemoglobin-like genes. This revealed that the tail-to-tail organization and alternating pattern of the α and β hemoglobin genes are well conserved in both clusters, as well as that the Atlantic salmon genome houses substantially more hemoglobin genes, including non-Bohr β globin genes, than the genomes of other teleosts that have been sequenced. Conclusions We suggest that the most parsimonious evolutionary path leading to the present organization of the Atlantic salmon

Genomic organization and evolution of the Atlantic salmon hemoglobin repertoire

Science.gov (United States)

2010-01-01

Background The genomes of salmonids are considered pseudo-tetraploid undergoing reversion to a stable diploid state. Given the genome duplication and extensive biological data available for salmonids, they are excellent model organisms for studying comparative genomics, evolutionary processes, fates of duplicated genes and the genetic and physiological processes associated with complex behavioral phenotypes. The evolution of the tetrapod hemoglobin genes is well studied; however, little is known about the genomic organization and evolution of teleost hemoglobin genes, particularly those of salmonids. The Atlantic salmon serves as a representative salmonid species for genomics studies. Given the well documented role of hemoglobin in adaptation to varied environmental conditions as well as its use as a model protein for evolutionary analyses, an understanding of the genomic structure and organization of the Atlantic salmon α and β hemoglobin genes is of great interest. Results We identified four bacterial artificial chromosomes (BACs) comprising two hemoglobin gene clusters spanning the entire α and β hemoglobin gene repertoire of the Atlantic salmon genome. Their chromosomal locations were established using fluorescence in situ hybridization (FISH) analysis and linkage mapping, demonstrating that the two clusters are located on separate chromosomes. The BACs were sequenced and assembled into scaffolds, which were annotated for putatively functional and pseudogenized hemoglobin-like genes. This revealed that the tail-to-tail organization and alternating pattern of the α and β hemoglobin genes are well conserved in both clusters, as well as that the Atlantic salmon genome houses substantially more hemoglobin genes, including non-Bohr β globin genes, than the genomes of other teleosts that have been sequenced. Conclusions We suggest that the most parsimonious evolutionary path leading to the present organization of the Atlantic salmon hemoglobin genes involves

[Saints and illnesses in faith and paleopathological evidences].

Science.gov (United States)

Fulcheri, Ezio

2006-01-01

The exhumation of the natural mummies of Beata Margherita di Savoia (1390-1464) and of St. Caterina Fieschi Adorno (1447-1510) has not been followed by autopsy or histological exams. The aim has been to preserve the integrity of the saints' corpses. We have thus proceeded to a confrontation between data gathered from chronicles and hagiographies and radiological exams and macroscopic observations. The article aims at establishing a model for a multidisciplinar approach to paleopathological research, integrating historical research and medical knowledge.

Symbiotic and nonsymbiotic hemoglobin genes of Casuarina glauca

DEFF Research Database (Denmark)

Jacobsen-Lyon, K; Jensen, Erik Østergaard; Jørgensen, Jan-Elo

1995-01-01

Casuarina glauca has a gene encoding hemoglobin (cashb-nonsym). This gene is expressed in a number of plant tissues. Casuarina also has a second family of hemoglobin genes (cashb-sym) expressed at a high level in the nodules that Casuarina forms in a nitrogen-fixing symbiosis with the actinomycete...... of the Casuarina gene. The finding that the nonsymbiotic Casuarina gene is also correctly expressed in L. corniculatus suggests to us that a comparable non-symbiotic hemoglobin gene will be found in legume species. Udgivelsesdato: 1995-Feb...

Identification of a haptoglobin-hemoglobin complex in the Alaskan Least Cisco (Coregonus sardinella).

Science.gov (United States)

Wahl, S M; Boger, J K; Michael, V; Duffy, L K

1992-01-01

The hemoglobin and a hemoglobin binding protein have been characterized in the Arctic fish (Coregonus sardinella). The evolutionary significance of the hemoglobin and plasma protein differences between fish and mammals is still unresolved. Blood samples from the Alaskan Least Cisco were separated into plasma and hemoglobin fractions and the proteins in these fractions were analyzed both by alkaline agarose gel electrophoresis, by isolelectric focusing, and by capillary electrophoresis. Staining the plasma proteins gels with o-dianisidine revealed hemoglobin containing protein complexes. A hemoglobin-containing band was observed in hemolyzed plasma which did not migrate with free hemoglobin, and is believed to be hemoglobin-haptoglobin complex. Size exclusion chromatography further characterized the hemoglobin as disassociating freely into dimers, and hemoglobin-haptoglobin complex having a molecular weight greater then 200,000 daltons.

Radiation - induced changes in the optical properties of hemoglobin molecule

International Nuclear Information System (INIS)

Selim, N.S; El-Marakby, S.M.

2009-01-01

Adult male albino rats were exposed to different doses of gamma radiation from Cs-137 source. Hemoglobin samples were analyzed 24 hrs after irradiation. The UV-visible spectrum of hemoglobin molecule was measured in the range 200 to 700 nm. The overall spectrum of the hemoglobin molecule showed hypochromicity that increased with dose increase. To investigate the effect of radiation on the hemoglobin molecule, different parameters of the spectrum were calculated: molar absorption coefficient, absorption cross section, transition dipole moment , dipole length, the optical energy gap and activation energy for each characteristic peak. The obtained results revealed that the radiation effect can induce rearrangement of the transition dipole moments and change molecular energy levels of the hemoglobin molecule

Hemoglobin levels in persons with depressive and/or anxiety disorders

NARCIS (Netherlands)

Lever-van Milligen, Bianca A.; Vogelzangs, Nicole; Smit, Johannes H.; Penninx, Brenda W. J. H.

Objective: Both low and high hemoglobin levels lead to more physical diseases, and both are linked to mortality. Low hemoglobin, often classified as anemia, has also been linked to more depressive symptoms, but whether both hemoglobin extremes are associated with depressive disorder and potentially

[On the medical and publishing activities of the community of Saint Eugene].

Science.gov (United States)

2012-01-01

The article deals with the role the physicians played in organization and functioning of the Community of Saint Eugene in St. Petersburg in 1882-1918. The typography production of the Community being of interest for history of medicine is examined.

Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis

DEFF Research Database (Denmark)

Wang, Yaping; Elhiti, Mohamed; Hebelstrup, Kim

2011-01-01

Over the past few years non-symbiotic plant hemoglobins have been described in a variety of plant species where they fulfill several functions ranging from detoxification processes to basic aspects of plant growth and post-embryonic development. To date no information is available on the role...... of hemoglobins during invitro morphogenesis. Shoot organogenesis was induced in Arabidopsis lines constitutively expressing class 1, 2 and 3 hemoglobins (GLB1, 2 and 3) and lines in which the respective genes were either downregulated by RNAi (GLB1) or knocked out (GLB2 and GLB3). The process was executed......, 15, and 16), feed-back repressors of the cytokinin pathway, was repressed in both hemoglobin over-expressors whereas that of several Type-B ARRs (ARR2, 12, and 13), transcription activators of cytokinin-responsive genes, was induced. Such changes enhanced the sensitivity of the root explants...

To build an environmental quality building. Evaluation: the HQE secondary school of Pic Saint Loup realized by the region; Construire un batiment respectueux de l'environnement. Retour d'experience: le Lycee HQE du Pic Saint Loup realise par la Region

Energy Technology Data Exchange (ETDEWEB)

Denicourt, Ch.

2004-07-01

This document presents the action realized in Pic Saint Loup secondary school, concerning the program management of an environmental quality building (HQE). The 8 chapters details the realization of the HQE building, the project planing of a HQE building, the Pic Saint Loup project, the operation beginning, the planing implementing, the project feasibility evaluation, the program redaction and the time and cost evaluation. (A.L.B.)

Studies on radiation induced changes in bovine hemoglobin type A

International Nuclear Information System (INIS)

Wdzieczak, J.; Duda, W.; Leyko, W.

1978-01-01

In this paper the structural and functional changes of gamma irradiated bovine hemoglobin are presented. Aqueous solutions/1%/of HbO 2 were irradiated in air with doses ranging from 1 to 4 Mrad. Isoelectric focusing indicated change of the charge of irradiated hemoglobin. The isoelectric point of hemoglobin was displaced towards more acid values with increasing doses, up from 1 Mrad. Fingerprint analysis and peptide column chromatography of irradiated hemoglobin demonstrated disturbances increasing with the dose. These changes were confirmed by amino acid analysis which showed that Cys, Met, Trp, His, Pro and Tyr residues were destroyed or modified following irradiation. At doses exceeding 1 Mrad the irradiated solutions of hemoglobin showed a decrease of heme-heme interaction and an increase of affinity for oxygen. Differences observed in oxygen-dissociation curves seem to be correlated with the radiation induced destruction of amino acid residues which are responsible for the functional properties of hemoglobin. (auth.)

Das Weiblich-Männliche in der saint-simonistischen Debatte um 1830

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Paola Ferruta

2009-12-01

Full Text Available D’une part, ma contribution interroge les concepts saint-simoniens de « masculin » et de « féminin » en se basant sur le cas concret de Pierre Leroux, adepte du saintsimonisme dès 1830 à Paris ; d’autre part, elle revient sur la notion d’identité sexuée, ainsi que sur sa variabilité intrinsèque. L’initiative politique et théorétique de Leroux au sein du groupe saint-simonien présente un intérêt historique, car elle a notamment participé à la diffusion au cours du XIXe siècle des croyances en la métempsychose et en des « vérités mystiques ». Ces « vérités mystiques » ont contribué à définir les identités de « genre » dans la pensée de Leroux et à façonner autour de 1840 son concept de « solidarité ». Or la question d’une « politique de la solidarité », et particulièrement de la « solidarité de genre » – c’est-à-dire d’une solidarité féminine qui ne fasse pas du féminisme une politique identitaire – est réactualisée dans le débat contemporainDie saint-simonistischen Begriffe „männlich“ und „weiblich“ sollen hier anhand des Falles von Pierre Leroux, einem Pariser Aktivisten des Saint-Simonismus, betrachtet werden. Es wird u. a. dessen Vorstellung von geschlechtlicher Identität und ihrer inhärenten Variabilität ins Auge gefasst. Die politische und theoretische Initiative Leroux’ ist insofern von historischem Interesse als sie zur Verbreitung des Glaubens an die Metempsychose und an „mystische Wahrheiten“ beitrug. Diese „mystischen Wahrheiten“ steuerten in Leroux’ Denken zur Definition der Geschlechteridentitäten bei und formten um 1840 seinen Begriff von „Solidarität“. Die Frage der „Politik der Solidarität“ ist heute noch aktuell, insbesondere einer „Geschlechtsidentität“, d. h. einer weiblichen Solidarität, die den Feminismus nicht als Identitätspolitik artikuliert.

Hemoglobin Values During Pregnancy | Leffler | Nigerian Medical ...

African Journals Online (AJOL)

It is known that the iron turnover in expectant mothers is up to three times that of an average adult. This is reflected in lower hemoglobin levels. The study showed that hemoglobin levels can be maintained by taking Bio-Strath®, provided that the patients' diet contains adequate fresh fruits and vegetables, whole grains, lean ...

Development of flood-inundation maps for the Mississippi River in Saint Paul, Minnesota

Science.gov (United States)

Czuba, Christiana R.; Fallon, James D.; Lewis, Corby R.; Cooper, Diane F.

2014-01-01

Digital flood-inundation maps for a 6.3-mile reach of the Mississippi River in Saint Paul, Minnesota, were developed through a multi-agency effort by the U.S. Geological Survey in cooperation with the U.S. Army Corps of Engineers and in collaboration with the National Weather Service. The inundation maps, which can be accessed through the U.S. Geological Survey Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/ and the National Weather Service Advanced Hydrologic Prediction Service site at http://water.weather.gov/ahps/inundation.php, depict estimates of the areal extent and depth of flooding corresponding to selected water levels (stages) at the U.S. Geological Survey streamgage at the Mississippi River at Saint Paul (05331000). The National Weather Service forecasted peak-stage information at the streamgage may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation. In this study, flood profiles were computed for the Mississippi River by means of a one-dimensional step-backwater model. The hydraulic model was calibrated using the most recent stage-discharge relation at the Robert Street location (rating curve number 38.0) of the Mississippi River at Saint Paul (streamgage 05331000), as well as an approximate water-surface elevation-discharge relation at the Mississippi River at South Saint Paul (U.S. Army Corps of Engineers streamgage SSPM5). The model also was verified against observed high-water marks from the recent 2011 flood event and the water-surface profile from existing flood insurance studies. The hydraulic model was then used to determine 25 water-surface profiles for flood stages at 1-foot intervals ranging from approximately bankfull stage to greater than the highest recorded stage at streamgage 05331000. The simulated water-surface profiles were then combined with a geographic information system digital elevation model, derived from high-resolution topography

A restoration and conservation project of the “Saint Dominic” Monastery in Soriano Calabro, Italy

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Nazzareno Davolos

2008-04-01

Full Text Available The article is extracted from the Master Thesis about Restoration of the Monuments at “La Sapienza”, University of the Studies of Rome. The Monastery was one of the most important of the Dominican Order in Europe. The building dates back to year 1510, it broke down almost completely in the earthquake of year 1659. It was rebuilt following the architect B. Presti’s project. At the present time the “Saint Domenic” Monastery largely seems a ruin, mainly as a consequence of the cat- N. Davolos - A restoration and conservation project of the ÒSaint DominicÓ Monastery... 185 astrophic earthquake in 1783. However, with the new look that has acquired, it still preserves an extraordinary charm that continues to astonish. The project followed the trend of the critical and conservative restoration with the safeguard of the artistic and figurative data of the “Saint Domenic” Monastery which has been analysed through an accurate historical-critical work. The idea is to preserve and transmit to the future generations the “Saint Domenic” monument in his authenticity, in its formal and material consistence, with the marks of the passing of time. The abstracts reports only the most important aspects which are representative of the project. Besides the historical analysis, laboratory examination have been executed on the material used in the construction of the ancient facade through the observation of thin sections by optic microscope and with the realization of spectrum of X-ray diffraction, in order to know the nature and composition, the state of preservation and the kinds of deterioration to support an appropriate intervention of restoration.

THE APOSTLE PAUL: THEOLOGIAN-MISSIONARY AND PASTOR-THEOLOGIAN. TOWARDS A DEEPER UNDERSTANDING OF SAINT PAUL

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Eckhart Schnabel

2013-04-01

Full Text Available This Article is an attempt to shed light on a few texts of the acts of the Apostles and of the epistles which are viewed as sources documenting the life and activity of Saint Paul. The author attempts to settle a number of questions regarding the authenticity of sources illustrating the life of Saint Paul. Similar questions are often raised by those who write and publish guides and textbooks for the teaching of the Pauline corpus. The author agrees with the overwhelming positive judgment of the academic world regarding the authenticity of the pastoral epistles, but at the same time he notes a number of objections to their authenticity proposed by the same forum: inconsistencies within the vocabulary of the texts themselves, their linguistic style, and their theological argumentation. The author concludes that these objections are inconclusive and the features enumerated by those who object constitute in reality only minor exceptions, inconsequential when taking into account the entire Pauline corpus. The author also examines the authenticity and historical accuracy of the account of the life of Saint Paul as given in the Acts of the Apostles. A few Biblicists claim that the account in Acts is merely legendary and is to be regarded as a type of early Christian folklore. The author disagrees with this opinion, backing up his position with the evidence that both the Acts of the Apostles and the Pastoral Epistles agree in historical detail and background. The author concludes with a further consideration regarding the correct method guiding biblical research on Saint Paul. He opines that such research must view the biblical texts in a correct historical perspective

Clinical, hematological and genetic data of a cohort of children with hemoglobin SD

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Paulo do Val Rezende

Full Text Available ABSTRACT INTRODUCTION: The hemoglobin FSD is very uncommon in newborn screening programs for sickle cell disease. In the program of Minas Gerais, Brazil, the clinical course of children with hemoglobin SD was observed to be heterogeneous. The objective of this study was to estimate the incidence (1999-2012 and to describe the natural history of a cohort of newborns with hemoglobin SD. METHODS: Isoelectric focusing was the primary method used in newborn screening. Polymerase chain reaction-restriction fragment length polymorphism and gene sequencing were used to identify mutant alleles and for haplotyping. Gap-polymerase chain reaction was used to detect alpha-thalassemia. RESULTS: Eleven cases of hemoglobin S/D-Punjab and eight of Hb S-Korle Bu were detected. Other variants with hemoglobin D mobility were not identified. All hemoglobin D-Punjab and hemoglobin Korle Bu alleles were associated with haplotype I. Among the children with hemoglobin S/D-Punjab, there were four with the ßS CAR haplotype, six with the Benin haplotype, and one atypical. Results of laboratory tests for hemoglobin S/D-Punjab and hemoglobin S-Korle Bu were: hemoglobin 8.0 and 12.3 g/dL (p-value <0.001, leukocyte count 13.9 × 109/L and 10.5 × 109/L (p-value = 0.003, reticulocytes 7.5% and 1.0% (p-value <0.001, hemoglobin F concentration 16.1% and 6.9% (p-value = 0.001 and oxygen saturation 91.9% and 97% (p-value = 0.002, respectively. Only hemoglobin S/D-Punjab children had acute pain crises and needed blood transfusions or hydroxyurea. Those with the Benin ßS haplotype had higher total hemoglobin and hemoglobin F concentrations compared to the CAR haplotype. Transcranial Doppler was normal in all children. CONCLUSION: The clinical course and blood cell counts of children with hemoglobin S/D-Punjab were very similar to those of hemoglobin SS children. In contrast, children with hemoglobin S-Korle Bu had clinical course and blood cell counts like children with the sickle

Recherche sur les sous-declarations de décés, Saint Marc, Haiti, 2012

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Stela Nazareth Meneghel

2014-11-01

Full Text Available Ce travail se réfère à une étude exploratoire menée dans la ville de Saint Marc, une commune d'environ 257,863 habitants en 2012, localisée dans le département d'Artibonite/Haïti. L'objectif est rechercher le flux d'informations concernant le système de mortalité et l'existence de non-déclarations de décès. Les décès survenus dans le mois de janvier 2013 ont fait l'objet d'une enquête dans cinq institutions de la ville de Saint Marc: l'Hôpital Saint Nicolas (HSN, la Mairie, le Bureau d'État Civil (BEC, le Cimetière et la Cathédrale. L'hôpital a été l'institution où l'on a rencontré le plus grand nombre de décès enregistrés. On a procédé au comptage des décès, à partir de la liste nominale des registres hospitaliers, en ajoutant les décè...

Oxidative stress in preeclampsia and the role of free fetal hemoglobin

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Stefan Rocco Hansson

2015-01-01

Full Text Available Preeclampsia is a leading cause of pregnancy complications and affects 3–7 % of pregnant women. This review summarizes the current knowledge of a new potential etiology of the disease, with a special focus on hemoglobin-induced oxidative stress. Furthermore, we also suggest hemoglobin as a potential target for therapy. Gene and protein profiling studies have shown increased expression and accumulation of free fetal hemoglobin in the preeclamptic placenta. Predominantly due to oxidative damage to the placental barrier, fetal hemoglobin leaks over to the maternal circulation. Free hemoglobin and its metabolites are toxic in several ways; a ferrous hemoglobin (Fe2+ binds strongly to the vasodilator nitric oxide and reduces the availability of free nitric oxide, which results in vasoconstriction, b hemoglobin (Fe2+ with bound oxygen spontaneously generates free oxygen radicals and c the heme groups create an inflammatory response by inducing activation of neutrophils and cytokine production. The endogenous protein α1-microglobulin, with radical and heme binding properties, has shown both ex vivo and in vivo to have the ability to counteract free hemoglobin-induced placental and kidney damage. Oxidative stress in general, and more specifically fetal hemoglobin-induced oxidative stress, could play a key role in the pathology of preeclampsia seen both in the placenta and ultimately in the maternal endothelium.

Conformational changes in hemoglobin triggered by changing the iron charge

International Nuclear Information System (INIS)

Croci, S.; Achterhold, K.; Ortalli, I.; Parak, F. G.

2008-01-01

In this work the hemoglobin conformational changes induced by changing the iron charge have been studied and compared with Myoglobin. Moessbauer spectroscopy was used to follow the change of the iron conformation. In order to compare the conformational relaxation of hemoglobin and myoglobin, and to study a possible influence of the quaternary structure, an intermediate metastable state of hemoglobin has been created by low temperature X-ray irradiation of methemoglobin. The irradiation reduces the Fe(III) of the heme groups to Fe(II) Low Spin, where the water is still bound on the sixth coordination. Heating cycles performed at temperatures from 140 K to 200 K allow the molecules to overcome an activation energy barrier and to relax into a stable conformation such as deoxy-hemoglobin or carboxy-hemoglobin, if CO is present. Slightly different structures (conformational substates) reveal themselves as a distribution of energy barriers (ΔG). The distribution of the activation energy, for the decay of the Fe(II) Low Spin intermediate, has been fitted with a Gaussian. For comparison, published myoglobin data were re-analysed in the same way. The average energy value at characteristic temperature is very similar in case of myoglobin and hemoglobin. The larger Gaussian energy distribution for myoglobin with respect to hemoglobin shows that more conformational substates are available. This may be caused by a larger area exposed to water. In hemoglobin, part of the surface of the chains is not water accessible due to the quaternary structure.

Marées et touristes au Mont-Saint-Michel

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Fernand Verger

2001-09-01

Full Text Available L’imaginaire collectif fait la plus grande place au phénomène des grandes marées dans la baie du Mont-Saint-Michel, mais la fréquentation touristique n’est que faiblement déterminée par l’occurrence des grandes marées, malgré l’existence d’un public spécifique, relativement peu nombreux, attiré par le spectacle du mascaret.

A thermodynamical measure of cooperativity: application to hemoglobin

International Nuclear Information System (INIS)

Jacchieri, S.G.; Ferreira, R.C.

1984-01-01

A comparative analysis of the heat requirements for dioxygen exchange is made for hemoglobin and myoglobin, the latter taken as the prototype of the vertebrate hemoglobin's ancestor. it is shown that cooperativity manifests itself also in terms of energy utilization. (Author) [pt

Saint-Lupicin (Jura, étude de bâti de l’église Notre-Dame

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Aurélia Bully

2007-08-01

Full Text Available Modalités de l’opération et problématiquesL’église de Saint-Lupicin fait l’objet depuis mai 2006 d’un important chantier de restauration sous la conduite de P. Barnoud, Architecte en chef des Monuments historiques. Les travaux portent essentiellement sur le remplacement de la toiture de la nef dont l’état de dégradation menaçait la pérennité de l’ouvrage. Après que la maîtrise d’ouvrage (commune de Saint-Lupicin eut choisi de restituer une charpente à quatre pans dans l’esprit du parti d’ori...

Consistent initial conditions for the Saint-Venant equations in river network modeling

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C.-W. Yu

2017-09-01

Full Text Available Initial conditions for flows and depths (cross-sectional areas throughout a river network are required for any time-marching (unsteady solution of the one-dimensional (1-D hydrodynamic Saint-Venant equations. For a river network modeled with several Strahler orders of tributaries, comprehensive and consistent synoptic data are typically lacking and synthetic starting conditions are needed. Because of underlying nonlinearity, poorly defined or inconsistent initial conditions can lead to convergence problems and long spin-up times in an unsteady solver. Two new approaches are defined and demonstrated herein for computing flows and cross-sectional areas (or depths. These methods can produce an initial condition data set that is consistent with modeled landscape runoff and river geometry boundary conditions at the initial time. These new methods are (1 the pseudo time-marching method (PTM that iterates toward a steady-state initial condition using an unsteady Saint-Venant solver and (2 the steady-solution method (SSM that makes use of graph theory for initial flow rates and solution of a steady-state 1-D momentum equation for the channel cross-sectional areas. The PTM is shown to be adequate for short river reaches but is significantly slower and has occasional non-convergent behavior for large river networks. The SSM approach is shown to provide a rapid solution of consistent initial conditions for both small and large networks, albeit with the requirement that additional code must be written rather than applying an existing unsteady Saint-Venant solver.

Three saints with deformed extremities in an Italian Renaissance altarpiece.

Science.gov (United States)

Albury, W R; Weisz, G M

2017-03-01

A fifteenth-century Florentine altarpiece painted by the Pollaiuolo brothers, Antonio (1433-1498) and Piero (1443-1496), shows three saints with evident deformities of the hands and feet. The pathologies concerned are tentatively identified, and various rationales for their presence in the painting are discussed. Of particular importance is the location of the altarpiece in a chapel which houses the tomb of the Cardinal of Portugal, Prince James of Lusitania (1433-1459). It is argued that both the artistic style of the day and the religious symbolism of the Cardinal's funeral chapel contributed to the artists' decision to portray the saints with deformities. An unnatural curvature of the fifth finger was apparently considered elegant in fifteenth-century paintings, and the depiction of bare feet with hallux valgus gave them a shape which approximated and could have been caused by fashionable pointed shoes. But in addition, deformities in religious art could be symbolic of suffering and martyrdom, a theme which the Cardinal's chapel emphasised in a number of ways. It is suggested therefore that the Pollaiuolo altarpiece reconciles these two disparate factors, portraying genuine deformities in a way that was artistically stylish and symbolically meaningful.

INTRINSIC REGULATION OF HEMOGLOBIN EXPRESSION BY VARIABLE SUBUNIT INTERFACE STRENGTHS

Science.gov (United States)

Manning, James M.; Popowicz, Anthony M.; Padovan, Julio C.; Chait, Brian T.; Manning, Lois R.

2012-01-01

SUMMARY The expression of the six types of human hemoglobin subunits over time is currently considered to be regulated mainly by transcription factors that bind to upstream control regions of the gene (the “extrinsic” component of regulation). Here we describe how subunit pairing and further assembly to tetramers in the liganded state is influenced by the affinity of subunits for one another (the “intrinsic” component of regulation). The adult hemoglobin dimers have the strongest subunit interfaces and the embryonic hemoglobins are the weakest with fetal hemoglobins of intermediate strength, corresponding to the temporal order of their expression. These variable subunit binding strengths and the attenuating effects of acetylation contribute to the differences with which these hemoglobin types form functional O2-binding tetramers consistent with gene switching. PMID:22129306

Diffusion coefficients of oxygen and hemoglobin as obtained simultaneously from photometric determination of the oxygenation of layers of hemoglobin solutions

NARCIS (Netherlands)

Spaan, J. A.; Kreuzer, F.; van Wely, F. K.

1980-01-01

The oxygenation of layers of deoxygenated hemoglobin solutions after a sudden exposure to a gas containing oxygen at a partial pressure P1 has been studied by a photometric method. Layer thicknesses varied between 50 and 250 micron, hemoglobin concentrations between 0.1 and 0.34kg/l, and oxygen

Mechanisms of the adjuvant effect of hemoglobin in experimental peritonitis. VII. Hemoglobin does not inhibit clearance of Escherichia coli from the peritoneal cavity

International Nuclear Information System (INIS)

Dunn, D.L.; Barke, R.A.; Lee, J.T. Jr.; Condie, R.M.; Humphrey, E.W.; Simmons, R.L.

1983-01-01

Hemoglobin has been shown to be a potent adjuvant in experimental Escherichia coli peritonitis, although a satisfactory mechanistic rationale is still obscure. Hemoglobin has been thought to impair intraperitoneal neutrophil function, delay clearance of bacteria from the peritoneal cavity by the normal absorptive mechanisms, or directly enhance bacterial growth. Using highly purified stroma-free hemoglobin (SFHgb), we have largely discounted any direct effect of hemoglobin on peritoneal white blood cell function. In the present study, we confirmed that uncontrolled proliferation of bacteria takes place in the presence of hemoglobin in the peritoneal cavity. Nonviable 5-iododeoxyuridine 125 I-labelled bacteria were then used to directly study peritoneal clearance kinetics, eliminating the problem of bacterial growth. SFHgb had no influence on the removal of intraperitoneal bacteria. The rate of bloodstream appearance of radiolabel was similar with or without intraperitoneal SFHgb. Thus, SFHgb does not prevent clearance of bacteria from the peritoneal cavity by interfering with normal host clearance mechanisms. SFHgb may act as a bacterial growth adjuvant, either by serving as a bacterial nutrient or by suitably modifying the environment so that extensive bacterial proliferation can occur. The latter hypothesis appears to be an area in which investigation concerning the adjuvant effect of hemoglobin may prove most fruitful

Síntese e caracterização de um novo composto obtido pela reação entre hidreto de trifenilestanho e ácido (±)-mandélico e avaliação de seu potencial biocida sobre o fungo Fusarium oxysporum f. sp. cubense Synthesis, characterization and evaluation of the biocide effect on the fungus Fusarium oxysporum f. sp. cubense of a new compound obtained by reaction of triphenyltin hydride and (±)-mandelic acid

OpenAIRE

Roberto Santos Barbiéri; Elysio Prado de Lima; Samuel Ferreira da Silva; Allan Kardec Carlos Dias; Maria das Graças Cardoso; Vilma Reis Terra

2006-01-01

O presente artigo refere-se à síntese e caracterização de um novo composto organoestânico, pela reação de ácido (±)-mandélico e hidreto de trifenilestanho, em meio de acetonitrila e sob refluxo, [(C6H5)2SnMand 2] {Mand = C6H5CH(OH)COO], identificado por análise elementar de carbono e hidrogênio, espectroscopia no infravermelho e espectrometria de massa de alta resolução, para o qual foi proposta estrutura octaédrica com o grupo fenila em posição trans. Verificou-se que o composto apresenta aç...

Plant hemoglobins: Important players at the crossroads between oxygen and nitric oxide

DEFF Research Database (Denmark)

Gupta, Kapuganti J; Hebelstrup, Kim; Mur, Luis A J

2011-01-01

Plant hemoglobins constitute a diverse group of hemeproteins and evolutionarily belong to three different classes. Class 1 hemoglobins possess an extremely high affinity to oxygen and their main function consists in scavenging of nitric oxide (NO) at very low oxygen levels. Class 2 hemoglobins have...... at high O2 concentrations. Depending on their physical properties, hemoglobins belong either to hexacoordinate non-symbiotic or pentacoordinate symbiotic groups. Plant hemoglobins are plausible targets for improving resistance to multiple stresses....

Le système du risque à Saint-Martin (Petites Antilles françaises

Directory of Open Access Journals (Sweden)

Virginie Duvat

2008-11-01

Full Text Available L’île de Saint-Martin constitue un terrain particulièrement intéressant pour étudier le système du risque naturel et les conditions de la co-évolution territoire/catastrophes. Les mécanismes qui s’y observent renforcent sa vulnérabilité et l’efficacité du retour d’expérience est affaiblie par des blocages d’ordre politique et juridique.The island of Saint-Martin is an interesting case study for a systemic approach applied to cyclone hazards and the co-evolution of territory and disaster management. The mecanisms which can be observed increase its vulnerability and the progress of hazard management policies is weakened by political and juridical bottlenecks.

Compressive strength evolution of thermally-stressed Saint Maximin limestone.

Science.gov (United States)

Farquharson, J.; Griffiths, L.; Baud, P.; Wadsworth, F. B.; Heap, M. J.

2017-12-01

The Saint Maximin quarry (Oise, France) opened in the early 1600s, and its limestone has been used extensively as masonry stone, particularly during the classical era of Parisian architecture from the 17th century onwards. Its widespread use has been due to a combination of its regional availability, its high workability, and its aesthetic appeal. Notable buildings completed using this material include sections of the Place de la Concorde and the Louvre in Paris. More recently, however, it has seen increasing use in the construction of large private residences throughout the United States as well as extensions to private institutions such as Stanford University. For any large building, fire hazard can be a substantial concern, especially in tectonically active areas where catastrophic fires may arise following large-magnitude earthquakes. Typically, house fires burn at temperatures of around 600 °C ( 1000 F). Given the ubiquity of this geomaterial as a building stone, it is important to ascertain the influence of heating on the strength of Saint Maximin limestone (SML), and in turn the structural stability of the buildings it is used in. We performed a series of compressive tests and permeability measurements on samples of SML to determine its strength evolution in response to heating to incrementally higher temperatures. We observe that the uniaxial compressive strength of SML decreases from >12 MPa at room temperature to 400 °C). We anticipate that this substantial weakening is in part a result of thermal microcracking, whereby changes in temperature induce thermal stresses due to a mismatch in thermal expansion between the constituent grains. This mechanism is compounded by the volumetric increase of quartz through its alpha - beta transition at 573 °C, and by the thermal decomposition of calcite. To track the formation of thermal microcracks, we monitor acoustic emissions, a common proxy for microcracking, during the heating of an SML sample. The

Operator modeling of a loss-of-pumping accident using MicroSAINT

International Nuclear Information System (INIS)

Olsen, L.M.

1992-01-01

The Savannah River Laboratory (SRL) human factors group has been developing methods for analyzing nuclear reactor operator actions during hypothetical design-basis accident scenarios. The SRL reactors operate at a lower temperature and pressure than power reactors resulting in accident sequences that differ from those of power reactors. Current methodology development is focused on modeling control room operator response times dictated by system event times specified in the Savannah River Site Reactor Safety Analysis Report (SAR). The modeling methods must be flexible enough to incorporate changes to hardware, procedures, or postulated system event times and permit timely evaluation. The initial model developed was for the loss-of-pumping accident (LOPA) because a significant number of operator actions are required to respond to this postulated event. Human factors engineers had been researching and testing a network modeling simulation language called MicroSAINT to simulate operators' personal and interpersonal actions relative to operating system events. The LOPA operator modeling project demonstrated the versatility and flexibility of MicroSAINT for modeling control room crew interactions

Molecular Mechanism of AHSP-Mediated Stabilization of Alpha-Hemoglobin

Energy Technology Data Exchange (ETDEWEB)

Feng,L.; Gell, D.; Zhou, S.; Gu, L.; Kong, Y.; Li, J.; Hu, M.; Yan, N.; Lee, C.; et al.

2005-01-01

Hemoglobin A (HbA), the oxygen delivery system in humans, comprises two alpha and two beta subunits. Free alpha-hemoglobin (alphaHb) is unstable, and its precipitation contributes to the pathophysiology of beta thalassemia. In erythrocytes, the alpha-hemoglobin stabilizing protein (AHSP) binds alphaHb and inhibits its precipitation. The crystal structure of AHSP bound to Fe(II)-alphaHb reveals that AHSP specifically recognizes the G and H helices of alphaHb through a hydrophobic interface that largely recapitulates the alpha1-beta1 interface of hemoglobin. The AHSP-alphaHb interactions are extensive but suboptimal, explaining why beta-hemoglobin can competitively displace AHSP to form HbA. Remarkably, the Fe(II)-heme group in AHSP bound alphaHb is coordinated by the distal but not the proximal histidine. Importantly, binding to AHSP facilitates the conversion of oxy-alphaHb to a deoxygenated, oxidized [Fe(III)], nonreactive form in which all six coordinate positions are occupied. These observations reveal the molecular mechanisms by which AHSP stabilizes free alphaHb.

Biophysical Monitoring and dose response characteristics of irradiated hemoglobin

International Nuclear Information System (INIS)

Elshemey, W.M; Selim, N.S.; Desouky, O.

2003-01-01

The present work aims to move a step forward towards a deeper understanding of the scattering of x-ray, from lyophilized biological samples. Comparative study has been performed using LAXS and UV-visible spectrophotometry for monitoring the dose response characteristics of the hemoglobin molecule of irradiated blood. Blood samples were irradiated at doses ranging from 5 up to 100 Gy. Diluted hemoglobin solution was scanned in the UV- visible range (200-700 nm), and lyophilized hemoglobin was prepared for LAXS measurement. The radiation-induced changes in the hemoglobin structure have been evaluated. The LAXS profile of hemoglobin molecule is characterized by the presence of two peaks in the forward direction of scattering. These peaks were found to be sensitive to the variations in the molecular structure of a given sample. The obtained results suggest that the 1 s t peak, recorded at 4.65 o , is sensitive to the tertiary and quaternary structure of the globin part, while the major peak, recorded at 10.5 o , appeared to be related to its primary and secondary structure

Latter-Day Saint Women and Leadership: The Influence of Their Religious Worldview

Science.gov (United States)

Madsen, Susan R.

2016-01-01

The article examines theories, assumptions, concepts, experiences, and practices from the Latter-day Saints' (LDS, or the Mormons) religious worldview to expand existing theoretical constructs and implications of leadership development and education for women. The article elucidates LDS doctrine and culture regarding women and provides specific…

El diario de un escritor en Encuentro en Saint-Nazaire de Ricardo Piglia

Directory of Open Access Journals (Sweden)

Amor Arelis Hernández Peñaloza

2016-01-01

Full Text Available En este artículoestudiamos el género diario íntimo a través de la nouvelleEncuentro en Saint-Nazaire(1988 que contiene el ”Diario de un loco”del escritor ficticio Stephen Stevensen. Este uso ficcional del diario, dentro delmarco de la estructura de la obra literaria adquiere un sentido diferente porque renuncia a la comunicación de algo íntimo y secreto, para convertirse en literatura, en ”comunicación estética”. Asimismopretendemosevidenciar la importancia y la magnitud del género diario en la unidad narrativa de un autor como Ricardo Piglia. Junto a esto, el ejemplo de Encuentro en Saint- Nazairenos ayudará a mostrar las características de este tipo de prosa pigliana, que funciona como un laboratorio de la escritura.

21 CFR 522.1125 - Hemoglobin glutamer-200 (bovine).

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Hemoglobin glutamer-200 (bovine). 522.1125 Section... (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS IMPLANTATION OR INJECTABLE DOSAGE FORM NEW ANIMAL DRUGS § 522.1125 Hemoglobin glutamer-200 (bovine). (a) Specifications. Each 125 milliliter bag contains 13...

Expression of fully functional tetrameric human hemoglobin in Escherichia coli

International Nuclear Information System (INIS)

Hoffman, S.J.; Looker, D.L.; Roehrich, J.M.; Cozart, P.E.; Durfee, S.L.; Tedesco, J.L.; Stetler, G.L.

1990-01-01

Synthesis genes encoding the human α- and β-globin polypeptides have been expressed from a single operon in Escherichia coli. The α- and β-globin polypeptides associate into soluble tetramers, incorporate heme, and accumulate to >5% of the total cellular protein. Purified recombinant hemoglobin has the correct stoichiometry of α- and β-globin chains and contains a full complement of heme. Each globin chain also contains an additional methionine as an extension to the amino terminus. The recombinant hemoglobin has a C 4 reversed-phase HPLC profile essentially identical to that of human hemoglobin A 0 and comigrates with hemoglobin A 0 on SDS/PAGE. The visible spectrum and oxygen affinity are similar to that of native human hemoglobin A 0 . The authors have also expressed the α- and β-globin genes separately and found that the expression of the α-globin gene alone results in a marked decrease in the accumulation of α-globin in the cell. Separate expression of the β-globin gene results in high levels of insoluble β-globin. These observations suggest that the presence of α- and β-globin in the same cell stabilizes α-globin and aids the correct folding of β-globin. This system provides a simple method for expressing large quantities of recombinant hemoglobin and allows facile manipulation of the genes encoding hemoglobin to produce functionally altered forms of this protein

Propanil-induced methemoglobinemia and hemoglobin binding in the rat

Energy Technology Data Exchange (ETDEWEB)

McMillan, D.C.; McRae, T.A.; Hinson, J.A. (National Center for Toxicological Research, Jefferson, AR (USA))

1990-09-15

Administration of (ring-U-14C)propanil (3,4-dichloropropionanilide) to male Sprague-Dawley rats (30, 100, and 300 mg/kg, ip) increased the formation of methemoglobin at the two highest doses. Following a propanil dose of 100 mg/kg, methemoglobin formation attained a maximum level of 5% by 1.5 hr and declined to normal levels (approximately 2.5%) by 12 hr. Hemoglobin binding attained a maximum level of 50 pmol/mg protein by 12 hr, and remained constant for 24 hr. Following a propanil dose of 300 mg/kg, methemoglobin formation attained a maximum level of 24% by 4.5 hr, and declined to a level of 5% by 24 hr. Hemoglobin binding attained a maximum level of 425 pmol/mg protein by 12 hr, and remained constant for 24 hr. Hemoglobin binding was also detected at the lowest propanil dose (10 pmol/mg protein) even though methemoglobin formation was not observed. HPLC analysis of alkaline-treated hemoglobin from propanil-treated rats indicated the presence of one radiolabeled compound with the same HPLC retention time as 3,4-dichloraniline. These data are consistent with the concept that propanil is converted to N-hydroxy-3,4-dichloroaniline in the liver. Subsequently, this metabolite enters the erythrocyte and is oxidized by hemoglobin to 3,4-dichloronitrosobenzene with concomitant conversion of oxyhemoglobin to methemoglobin. The 3,4-dichloronitrosobenzene binds to cysteine residues on hemoglobin as the corresponding sulfinic acid amide adduct. These data suggest that human exposure to propanil may be monitored in the absence of observable toxicity by the analysis of propanil metabolites bound to hemoglobin.

Rice (Oryza) hemoglobins

Science.gov (United States)

Hemoglobins (Hbs) corresponding to non-symbiotic (nsHb) and truncated (tHb) Hbs have been identified in rice (Oryza). This review discusses the major findings from the current studies on rice Hbs. At the molecular level, a family of the nshb genes, consisting of hb1, hb2, hb3, hb4 and hb5, and a sin...

Concomitant use of the matrix strategy and the mand-model procedure in teaching graphic symbol combinations.

Science.gov (United States)

Nigam, Ravi; Schlosser, Ralf W; Lloyd, Lyle L

2006-09-01

Matrix strategies employing parts of speech arranged in systematic language matrices and milieu language teaching strategies have been successfully used to teach word combining skills to children who have cognitive disabilities and some functional speech. The present study investigated the acquisition and generalized production of two-term semantic relationships in a new population using new types of symbols. Three children with cognitive disabilities and little or no functional speech were taught to combine graphic symbols. The matrix strategy and the mand-model procedure were used concomitantly as intervention procedures. A multiple probe design across sets of action-object combinations with generalization probes of untrained combinations was used to teach the production of graphic symbol combinations. Results indicated that two of the three children learned the early syntactic-semantic rule of combining action-object symbols and demonstrated generalization to untrained action-object combinations and generalization across trainers. The results and future directions for research are discussed.

[Department of Otorhinolaryngology of the I.P. Pavlov Saint-Peterburg First State Medical University].

Science.gov (United States)

Karpishchenko, S A

This article is designed to report the results of the analysis ofacademic, scientific, and clinical activities of the Department of Otorhinolaryngology of the I.P. Pavlov Saint-Peterburg First State Medical University.

Saint Francis Xavier on Jesuit School Stages of the Bohemian Province

Czech Academy of Sciences Publication Activity Database

Bobková, Kateřina; Jacková, Magdaléna

2015-01-01

Roč. 25, č. 2 (2015), s. 135-155 ISSN 0515-3190 R&D Projects: GA ČR GPP406/12/P823 Institutional support: RVO:68378068 ; RVO:67985963 Keywords : St. Francis Xavier * Jesuit saints * Jesuit theatre * neolatin drama * school theatre * Avancini, Nicolaus * Engel, Arnoldus Subject RIV: AJ - Letters, Mass-media, Audiovision; AB - History (HIU-Y)

Evans Syndrome Complicated by Intratubular Hemoglobin Cast Nephropathy

Directory of Open Access Journals (Sweden)

Iván González

2017-01-01

Full Text Available Evans syndrome (ES is a rare autoimmune disorder whose exact pathophysiology is unknown. It is characterized by the simultaneous or subsequent development of autoimmune hemolytic anemia (AIHA and immune thrombocytopenia (ITP. Intravascular hemolysis, with hemoglobinemia, is known to produce acute kidney injury; however, the development of intratubular hemoglobin casts (hemoglobin cast nephropathy in the setting of acute hemolysis is uncommon. Likewise, the association of ES and acute renal failure is equally uncommon. We present a case of a 7-year-old girl with ES who developed acute kidney injury in the setting of intravascular hemolysis and had widespread intratubular hemoglobin casts.

21 CFR 864.7400 - Hemoglobin A2 assay.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Hemoglobin A2 assay. 864.7400 Section 864.7400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Kits and Packages § 864.7400 Hemoglobin A2...

Action of carbon monoxide on the affinity of hemoglobin for oxygen

Energy Technology Data Exchange (ETDEWEB)

Vanuxem, D.; Weiller, P.J.; Guillot, C.; Grimaud, C.

1982-01-01

The authors have studied the action of carbon monoxide on the affinity of hemoglobin for oxygen by measuring P50 in whole blood and in stripped hemoglobin before and after exposition of blood samples from heavy smokers and polycythemic patients with high levels of HbCO to hyperbaric oxygen (2.2 ata). The concentration of 2,3-diphosphoglycerate was normal although P50 was significantly lowered, not only in whole blood but also in stripped hemoglobin. Hyperbaric oxygen normalized P50 by removing CO radicals from stripped hemoglobin. This may indicate that CO radicals exert a direct action on the hemoglobin molecule, at least at the HbCO levels studied in this work.

Nitrosyl hemoglobins: EPR above 80 K

Energy Technology Data Exchange (ETDEWEB)

Wajnberg, E.; Bemski, G.; El-Jaick, L.J.; Alves, O.C.

1995-03-01

The EPR spectra of nitrosyl hemoglobin and myoglobin in different conditions (native, denatured and lyophilized), as well as of hematin-NO were obtained in the temperature range of 80 K-280 K. There is a substantial and reversible.decrease of the areas of the EPR spectra of all the hemoglobin samples above 150 K. The interpretation of the results implies the existence of two conformational states in thermal equilibrium only one of which is EPR detectable. Thermodynamical parameters are determined for the hexa and penta-coordinated cases. (author). 25 refs, 3 figs.

Nitrosyl hemoglobins: EPR above 80 K

International Nuclear Information System (INIS)

Wajnberg, E.; Bemski, G.; El-Jaick, L.J.; Alves, O.C.

1995-03-01

The EPR spectra of nitrosyl hemoglobin and myoglobin in different conditions (native, denatured and lyophilized), as well as of hematin-NO were obtained in the temperature range of 80 K-280 K. There is a substantial and reversible.decrease of the areas of the EPR spectra of all the hemoglobin samples above 150 K. The interpretation of the results implies the existence of two conformational states in thermal equilibrium only one of which is EPR detectable. Thermodynamical parameters are determined for the hexa and penta-coordinated cases. (author). 25 refs, 3 figs

Saints' Relics in Scuola di S. Giorgio degli Schiavoni: An Anti-Ottoman Pantheon

Directory of Open Access Journals (Sweden)

Ana Marinković

2018-04-01

Full Text Available The intensified military campaigns against the Ottomans in the second half of the fifteenth century palpably influenced the cult of saints venerated in Scuola di S. Giorgio degli Schiavoni, both by assimilating new saints, as well as strenghtening the existing cults through indulgences and relic acquisitions. The initial pair of patron saints – St George and St Tryphon, the city patrons of Bar and Kotor – delineated the geographical area where the majority of confraternity members originated from, that is, the Venetian Albania. The inclusion of St Jerome, the patron of Dalmatia, to the confraternal pantheon by Cardinal Bessarion's indulgence issued in 1464, in addition to rounding up the holy patronage to all the Eastern Adriatic Venetian dependencies, also marked the beginning of a strong anti-Ottoman motivation in the scuola's hagiographic horizon. The triple endowment of the Scuola in 1502 – relic acquisition, granting of indulgence, and commission of Carpaccio's monumental cycle – continued to work on the same line, and added to the practical aspect of spiritual support to the members of Venetian troops provenient of the Eastern Adriatic. However, the saints whose relics are today kept in the altar of the lower hall, including the patron saint of the Republic of Dubrovnik, point to an even wider devotional agenda, possibly arranged after the Battle of Lepanto, as the comparative analysis of the reliquaries show.   Le campagne militari contro gli Ottomani intensificatesi nella seconda metà del XV secolo influenzarono palesemente il culto dei santi venerati nella Scuola di S. Giorgio degli Schiavoni, sia attraverso l'assimilazione di nuovi santi, sia tramite il rafforzamento del culto già esistente verso le indulgenze e l'impulso all'acquisizione di reliquie. La prima coppia di santi patroni - San Giorgio e San Trifone, patroni della città di Bar e Cattaro - delimitano l'area geografica da cui proviene la maggior parte dei membri

A spectroscopic study on the interaction between gold nanoparticles and hemoglobin

International Nuclear Information System (INIS)

Garabagiu, Sorina

2011-01-01

Highlights: ► The interaction was studied using UV–vis and fluorescence spectroscopy. ► Gold nanoparticles quench the fluorescence emission of hemoglobin solution. ► The binding and thermodynamic constants were calculated. ► Major impact: electrochemical applications of the complex onto a substrate. -- Abstract: The interaction between horse hemoglobin and gold nanoparticles was studied using optical spectroscopy. UV–vis and fluorescence spectra show that a spontaneous binding process occurred between hemoglobin and gold nanoparticles. The Soret band of hemoglobin in the presence of gold nanoparticles does not show significant changes, which proves that the protein retained its biological function. A shift to longer wavelengths appears in the plasmonic band of gold nanoparticles upon the attachment of hemoglobin molecules. Gold nanoparticles quench the fluorescence emission of tryptophan residues in the structure of hemoglobin. The Stern–Volmer quenching constant, the binding constant and the number of binding sites were also calculated. Thermodynamic parameters indicate that the binding was mainly due to hydrophobic interactions.

Human macrophage hemoglobin-iron metabolism in vitro

International Nuclear Information System (INIS)

Custer, G.; Balcerzak, S.; Rinehart, J.

1982-01-01

An entirely in vitro technique was employed to characterize hemoglobin-iron metabolism by human macrophages obtained by culture of blood monocytes and pulmonary alveolar macrophages. Macrophages phagocytized about three times as many erythrocytes as monocytes and six times as many erythrocytes as pulmonary alveolar macrophages. The rate of subsequent release of 59 Fe to the extracellular transferrin pool was two- to fourfold greater for macrophages as compared to the other two cell types. The kinetics of 59 Fe-transferrin release were characterized by a relatively rapid early phase (hours 1-4) followed by a slow phase (hours 4-72) for all three cell types. Intracellular movement of iron was characterized by a rapid shift from hemoglobin to ferritin that was complete with the onset of the slow phase of extracellular release. A transient increase in 59 Fe associated with an intracellular protein eluting with transferrin was also observed within 1 hour after phagocytosis. The process of hemoglobin-iron release to extracellular transferrin was inhibited at 4 degrees C but was unaffected by inhibitory of protein synthesis, glycolysis, microtubule function, and microfilament function. These data emphasize the rapidity of macrophage hemoglobin iron metabolism, provide a model for characterization of this process in vitro, and in general confirm data obtained utilizing in vivo animal models

Christian Feasts dedicated to Saints – an Interdisciplinary Perspective

Directory of Open Access Journals (Sweden)

Carmen-Maria Bolocan

2012-06-01

Full Text Available Our paper is an interdisciplinary approach, of linguistic and theological analysis of a few lexemes that define saints who became popular in the Romanian culture and spirituality. Our ancient church, a fundamental part of our culture, has always been a coagulant factor for the unity of the Romanians worldwide. And this is also proven by the use of the religious vocabulary in all the anthropological, territorial, socio-professional and socio-cultural variants of the national Romanian language.

Business plan of a food truck in Saint Petersburg

OpenAIRE

Zavyalova, Ekaterina

2017-01-01

The purpose of the thesis was to investigate how to write a proper business plan and then implement gained knowledge in creating a business plan of a food truck in Saint Petersburg. A business plan serves as a guide for an entrepreneur in the process of company establishment and enables to gain funding from potential investors. The primary objective was to study essential components of a business plan. The theoretical framework comprises of core issues on company establishment and desc...

The Greenland shark Somniosus microcephalus-Hemoglobins and ligand-binding properties.

Directory of Open Access Journals (Sweden)

Roberta Russo

Full Text Available A large amount of data is currently available on the adaptive mechanisms of polar bony fish hemoglobins, but structural information on those of cartilaginous species is scarce. This study presents the first characterisation of the hemoglobin system of one of the longest-living vertebrate species (392 ± 120 years, the Arctic shark Somniosus microcephalus. Three major hemoglobins are found in its red blood cells and are made of two copies of the same α globin combined with two copies of three very similar β subunits. The three hemoglobins show very similar oxygenation and carbonylation properties, which are unaffected by urea, a very important compound in marine elasmobranch physiology. They display identical electronic absorption and resonance Raman spectra, indicating that their heme-pocket structures are identical or highly similar. The quaternary transition equilibrium between the relaxed (R and the tense (T states is more dependent on physiological allosteric effectors than in human hemoglobin, as also demonstrated in polar teleost hemoglobins. Similar to other cartilaginous fishes, we found no evidence for functional differentiation among the three isoforms. The very similar ligand-binding properties suggest that regulatory control of O2 transport may be at the cellular level and that it may involve changes in the cellular concentrations of allosteric effectors and/or variations of other systemic factors. The hemoglobins of this polar shark have evolved adaptive decreases in O2 affinity in comparison to temperate sharks.

Using the MWC model to describe heterotropic interactions in hemoglobin

Science.gov (United States)

Rapp, Olga

2017-01-01

Hemoglobin is a classical model allosteric protein. Research on hemoglobin parallels the development of key cooperativity and allostery concepts, such as the ‘all-or-none’ Hill formalism, the stepwise Adair binding formulation and the concerted Monod-Wymann-Changuex (MWC) allosteric model. While it is clear that the MWC model adequately describes the cooperative binding of oxygen to hemoglobin, rationalizing the effects of H+, CO2 or organophosphate ligands on hemoglobin-oxygen saturation using the same model remains controversial. According to the MWC model, allosteric ligands exert their effect on protein function by modulating the quaternary conformational transition of the protein. However, data fitting analysis of hemoglobin oxygen saturation curves in the presence or absence of inhibitory ligands persistently revealed effects on both relative oxygen affinity (c) and conformational changes (L), elementary MWC parameters. The recent realization that data fitting analysis using the traditional MWC model equation may not provide reliable estimates for L and c thus calls for a re-examination of previous data using alternative fitting strategies. In the current manuscript, we present two simple strategies for obtaining reliable estimates for MWC mechanistic parameters of hemoglobin steady-state saturation curves in cases of both evolutionary and physiological variations. Our results suggest that the simple MWC model provides a reasonable description that can also account for heterotropic interactions in hemoglobin. The results, moreover, offer a general roadmap for successful data fitting analysis using the MWC model. PMID:28793329

The impact of hemoglobin on the efficacy of phototherapy in hyperbilirubinemic infants

DEFF Research Database (Denmark)

Donneborg, Mette L; Vandborg, Pernille K; Hansen, Bo M

2017-01-01

BackgroundPhototherapy is the routine treatment for neonatal hyperbilirubinemia. Absorption of light in the skin transforms the native Z,Z-bilirubin to photobilirubins. This study investigates whether the hemoglobin concentration has an impact on efficacy of phototherapy, expressed by the decline...... decrease in TsB after 24 h was 121 (57-199) μmol/l; the median hemoglobin was 12.0 (7.0-14.7) mmol/l. There was a significant effect of hemoglobin concentration on the decrease in TsB of -3.61 μmol/mmol hemoglobin (P=0.022), after adjusting for initial TsB and postnatal age. That is, assuming the same...... initial TsB and postnatal age, for each mmol/l increase in hemoglobin, the decrease in TsB was 3.61 μmol/l smaller. In our hemoglobin range, the decrease in TsB is reduced by 28 μmol/l (23%).ConclusionIncreasing hemoglobin levels led to a decrease in the efficacy of phototherapy. Our data provide...

Comparative study of bedside and laboratory measurements of hemoglobin.

Science.gov (United States)

Krenzischek, D A; Tanseco, F V

1996-11-01

The purpose of this study was to examine the effects of variations in technique on measurements of hemoglobin level done at the bedside and to compare these results with laboratory measurements of hemoglobin. In accordance with hospital policy, procedure, and protocol, various techniques were used to obtain samples of capillary and venous blood and of blood from arterial and central venous catheters. Levels of hemoglobin were measured at the bedside and in the laboratory, and the results were compared. The Johns Hopkins Hospital adult postanesthesia care unit. A total of 187 blood samples were obtained from 62 adults who had undergone general surgery. Group I comprised 20 subjects with capillary and venous blood samples. Group II comprised 21 subjects with arterial blood samples. Group III comprised 21 subjects with central venous blood samples. The results showed that the amount of blood to be discarded before obtaining samples of arterial and central venous blood need not be any larger than double the dead space of the catheter, and that shaking the blood sample for 10 seconds was sufficient to mix the sample before measurement of hemoglobin levels. Results of bedside and laboratory measurements of hemoglobin level were comparable. Bedside measurement of hemoglobin increases efficiency in patient care, decreases risk of blood-transmitted infection for staff, and decreases cost to the patient. However, the persons who perform the assay must be responsible in adhering to the standard of practice to minimize errors in the measurements.

Prognostic value of hemoglobin concentration in radiotherapy for cancer of supraglottic larynx

International Nuclear Information System (INIS)

Tarnawski, Rafal; Skladowski, Krzysztof; Maciejewski, Boguslaw

1997-01-01

Purpose: The aim of this work is the estimation of correlations between hemoglobin concentration either before or after radiotherapy and local tumor control probability for laryngeal cancer. Methods and Materials: Retrospective analysis of 847 cases of laryngeal supraglottic squamous cell carcinoma treated with radiation alone was performed using maximum likelihood estimations, and step-wise logistic regression. All patients were in good initial performance status (Karnofsky index >70). The minimum follow-up time was 3 years. Results: Logistic regression showed that the hemoglobin concentration after radiotherapy is an important prognostic factor. There was a very strong correlation between hemoglobin concentration and tumor local control probability. Hemoglobin concentration at the beginning of radiotherapy does not correlate with treatment outcome, but any decrease of hemoglobin during therapy is a strong prognostic factor for treatment failure. Conclusions: Although regression models with many variables may be instable, the present results suggest that hemoglobin concentration after treatment is at least as important as overall treatment time. It was not possible to find out whether the low concentration of hemoglobin is an independent cause of low TCP or whether it reflects other mechanisms that may influence both hemoglobin level and the TCP

A nanocluster-based fluorescent sensor for sensitive hemoglobin detection.

Science.gov (United States)

Yang, Dongqin; Meng, Huijie; Tu, Yifeng; Yan, Jilin

2017-08-01

In this report, a fluorescence sensor for sensitive detection of hemoglobin was developed. Gold nanoclusters were first synthesized with bovine serum albumin. It was found that both hydrogen peroxide and hemoglobin could weakly quench the fluorescence from the gold nanoclusters, but when these two were applied onto the nanolcusters simultaneously, a much improved quenching was resulted. This enhancing effect was proved to come from the catalytic generation of hydroxyl radical by hemoglobin. Under an optimized condition, the quenching linearly related to the concentration of hemoglobin in the range of 1-250nM, and a limit of detection as low as 0.36nM could be obtained. This provided a sensitive means for the quantification of Hb. The sensor was then successfully applied for blood analyses with simple sample pretreatment. Copyright © 2017 Elsevier B.V. All rights reserved.

Temperature-dependent enthalpy of oxygenation in Antarctic fish hemoglobins

DEFF Research Database (Denmark)

Fago, A.; Wells, R.M.G.; Weber, Roy E.

1997-01-01

The effect of temperature on the oxygen-binding properties of the hemoglobins of three cold-adapted Antarctic fish species, Dissostichus mawsoni, Pagothenia borchgrevinki and Trematomus, sp., has been investigated under different pH values and buffer conditions. A clear non linear van't Hoff plot...... (logP(50) vs 1/T) of D. mawsoni hemoglobin indicates that the enthalpy of oxygenation (slope of the plot) is temperature dependent and that at high temperatures oxygen-binding becomes less exothermic. Nearly linear relationships were found in the hemoglobins of the other two species. The data were...... oxygen binding. The degree of the temperature dependence of the heat of oxygenation observed in these hemoglobins seems to reflect the differences in their allosteric effects rather than a specific molecular adaptation to low temperatures. Moreover, this study indicates that the disagreement between...

Hemoglobin Concentration and Risk of Incident Stroke in Community-Living Adults.

Science.gov (United States)

Panwar, Bhupesh; Judd, Suzanne E; Warnock, David G; McClellan, William M; Booth, John N; Muntner, Paul; Gutiérrez, Orlando M

2016-08-01

In previous observational studies, hemoglobin concentrations have been associated with an increased risk of stroke. However, these studies were limited by a relatively low number of stroke events, making it difficult to determine whether the association of hemoglobin and stroke differed by demographic or clinical factors. Using Cox proportional hazards analysis and Kaplan-Meier plots, we examined the association of baseline hemoglobin concentrations with incident stroke in the Reasons for Geographic and Racial Differences in Stroke (REGARDS) study, a cohort of black and white adults aged ≥45 years. A total of 518 participants developed stroke over a mean 7±2 years of follow-up. There was a statistically significant interaction between hemoglobin and sex (P=0.05) on the risk of incident stroke. In Cox regression models adjusted for demographic and clinical variables, there was no association of baseline hemoglobin concentration with incident stroke in men, whereas in women, the lowest (14.0 g/dL) quartiles of hemoglobin were associated with higher risk of stroke when compared with the second quartile (12.4-13.2 g/dL; quartile 1: hazard ratio, 1.59; 95% confidence interval, 1.09-2.31; quartile 2: referent; quartile 3: hazard ratio, 0.91; 95% confidence interval, 0.59-1.38; quartile 4: hazard ratio, 1.59; 95% confidence interval, 1.08-2.35). Similar results were observed in models stratified by hemoglobin and sex and when hemoglobin was modeled as a continuous variable using restricted quadratic spline regression. Lower and higher hemoglobin concentrations were associated with a higher risk of incident stroke in women. No such associations were found in men. © 2016 American Heart Association, Inc.

Nonenzymatic glycosylation of human hemoglobin at multiple sites

International Nuclear Information System (INIS)

Shapiro, R.; McManus, M.; Garrick, L.; McDonald, M.J.; Bunn, H.F.

1979-01-01

The most abundant minor hemoglobin component of human hemolysate is Hb A1c, which has glucose bound to the N-terminus of the beta chain by a ketoamine linkage. Hb A1c is formed slowly and continuously throughout the 120 day lifespan of the red cell. It can be synthesized in vitro by incubating purified hemoglobin with 14C-glucose. Other minor components, Hb A1a1 and Hb A1a2 are adducts of sugar phosphates at the N-terminus of the beta chain. Hb A1b contains an unidentified nonphosphorylated sugar at the beta N-terminus. In addition, a significant portion of the major hemoglobin component (Hb Ao) is also glycosylated by a glucose ketoamine linkage at other sites on the molecule, including the N-terminus of the alpha chain and the epsilon-amino group of several lysine residues on both the alpha and the beta chains. The results indicate that the interaction of glucose and hemoglobin is rather nonspecific and suggests that other proteins are modified in a similar fashion

Biophysical basis of hypoxic radioprotection by deoxygenated dextran-hemoglobin

International Nuclear Information System (INIS)

Wong, J.T.; Hill, R.P.

1986-01-01

Perfusion with deoxygenated dextran-hemoglobin provides an effective method for inducing hypoxic radioprotection of normal tissues during radiation treatment of tumors. In this study, the dependence of P50, the half-saturation pressure of oxygen binding to dextran-hemoglobin, was analyzed as a function of solution temperature and pH. The variation of attainable radioprotection with P50, and with the amount of collateral blood entering into the perfused region, was calculated. Upon perfusion of canine gracilis muscle with deoxygenated dextran-hemoglobin, a rapid onset of extensive venous hypoxia was observed

Histopathologic Study Following Administration of Liposome-Encapsulated Hemoglobin in the Normovolemic Rat

National Research Council Canada - National Science Library

Rudolph, Alan

1995-01-01

... bovine hemoglobin in the normovolemic rat. We have also examined the administration of the liposome vehicle, tetrameric bovine hemoglobin, and liposome encapsulated bovine hemoglobin that had been lyophilized with 300 mM trehalose...

Structure and stability of human hemoglobin microparticles prepared with a double emulsion technique.

Science.gov (United States)

Cedrati, N; Bonneaux, F; Labrude, P; Maincent, P

1997-09-01

Hemoglobin solutions can be used as blood substitutes but they present some disadvantages often due to their rapid removal from the bloodstream after injection. A possible way of overcoming this problem is to trap hemoglobin inside particles. This study deals with the preparation, structure and stability of poly(lactic acid) and ethylcellulose microparticles containing human hemoglobin obtained with a double emulsion technique. We investigated the manufacturing process of these particles in order to increase the encapsulation ratio of hemoglobin. For this purpose, some parameters involved in the procedure were optimized, such as hemoglobin concentration and duration of stirring: hemoglobin loading increases with its concentration in the preparation and well-defined stirring time avoids a leakage of hemoglobin. Hemoglobin concentration, surfactant concentration i.e. poly(vinylic alcohol), amounts of polymer and solvent (methylene chloride), duration and speed of stirring. The microparticles were prepared with satisfactory yields (60 to 73%). They were spherical and their mean size was lower than 200 microns. The functional properties of entrapped hemoglobin were studied. The encapsulation did not alter hemoglobin and the oxygen affinity of the hemoglobin remained unmodified (P50 about 13.9 mm Hg in a Bis-Tris buffer pH 7.4 at 37 degrees C). Moreover, only low levels of methemoglobin could be detected (less than 3%). Besides, about 90% of encapsulated hemoglobin could be released from microparticles, with a speed related to the internal structure of the particles. The prepared microparticles were stored during one month at +4 degrees C. No degradation of the particle structure occurred and the functional properties of hemoglobin were preserved. These particles could provide a potential source of oxygen in the field of biotechnologies but any application for a transfusional purpose would first require a drastic reduction in particle size.

Comportamiento de las ecuaciones de Saint-Venant en 1D y aproximaciones para diferentes condiciones en régimen permanente y variable

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Gloria Estefany Amarís Castro

2015-07-01

Full Text Available The importance of the behavior of the Saint Venant equations and its implications for flow analysis applied to models of hydraulic transit is the main theme of this research, where the basis of the applications and uses of these mathematical models is determined and the performance is evaluated these equations in 1D and possible approaches for different conditions of a real situation using computational numerical experimentation for a section of a prismatic channel with a hydraulic section, in cases where the channel slope presents three types of conditions: supercritical, subcritical and horizontal slope for a design rate. The main objective of this research is to find a demonstration of the applicability of the Saint Venant equations for different conditions of approaching this problem from the point of view of the solution of the Saint Venant equations in one dimension. Scheme simple solution that produce useful solutions to the needs of engineering and found to be within the sewer network analysis, many factors can affect the solution of the Saint Venant equations is proposed.

[Hemoglobins, XXXII. Analysis of the primary structure of the monomeric hemoglobin CTT VIIA (erythrocruorin) or Chironomus thummi thummi, Diptera (author's transl)].

Science.gov (United States)

Kleinschmidt, T; Braunitzer, G

1980-01-01

The dimeric hemoglobin CTT VIIA (erythrocruorin) was isolated from the hemolymph of the larva from Chironomus thummi thummi and purified by preparative polyacrylamide gel electrophoresis. Peptides obtained by limited tryptical digestion were sequenced by automatic Edman degradation. For the elucidation of the sequence in the C-terminal region of the chain, additional cleavages with proteinase of Staphylococcus aureus and chymotrypsin were necessary. CTT VIIA is compared with human beta-chains and other hemoglobins of Chironomus. The amino acid residues in the pocket are especially discussed. Most of them are invariant in all Chironomus hemoglobins, independent of the size of the heme pocket, which is normal in some components and enlarged in others.

Enhancing the IT Infrastructure at Saint Philip's Hospital: Point-of-Care Solutions

Science.gov (United States)

Naydenova, Iva; White, Bruce

2013-01-01

Healthcare has become a rapidly changing field. With the introduction of value-based purchasing to determine reimbursement of Medicare providers based on the quality of care in addition to outcomes in treatment, the environment is becoming ever more competitive. Saint Philip's Hospital is among the largest non-profit hospitals in the nation…

Cloned Hemoglobin Genes Enhance Growth Of Cells

Science.gov (United States)

Khosla, Chaitan; Bailey, James E.

1991-01-01

Experiments show that portable deoxyribonucleic acid (DNA) sequences incorporated into host cells make them produce hemoglobins - oxygen-binding proteins essential to function of red blood cells. Method useful in several biotechnological applications. One, enhancement of growth of cells at higher densities. Another, production of hemoglobin to enhance supplies of oxygen in cells, for use in chemical reactions requiring oxygen, as additive to serum to increase transport of oxygen, and for binding and separating oxygen from mixtures of gases.

FRET imaging of hemoglobin concentration in Plasmodium falciparum-infected red cells.

Directory of Open Access Journals (Sweden)

Alessandro Esposito

Full Text Available During its intraerythrocytic asexual reproduction cycle Plasmodium falciparum consumes up to 80% of the host cell hemoglobin, in large excess over its metabolic needs. A model of the homeostasis of falciparum-infected red blood cells suggested an explanation based on the need to reduce the colloid-osmotic pressure within the host cell to prevent its premature lysis. Critical for this hypothesis was that the hemoglobin concentration within the host cell be progressively reduced from the trophozoite stage onwards.The experiments reported here were designed to test this hypothesis by direct measurements of the hemoglobin concentration in live, infected red cells. We developed a novel, non-invasive method to quantify the hemoglobin concentration in single cells, based on Förster resonance energy transfer between hemoglobin molecules and the fluorophore calcein. Fluorescence lifetime imaging allowed the quantitative mapping of the hemoglobin concentration within the cells. The average fluorescence lifetimes of uninfected cohorts was 270+/-30 ps (mean+/-SD; N = 45. In the cytoplasm of infected cells the fluorescence lifetime of calcein ranged from 290+/-20 ps for cells with ring stage parasites to 590+/-13 ps and 1050+/-60 ps for cells with young trophozoites and late stage trophozoite/early schizonts, respectively. This was equivalent to reductions in hemoglobin concentration spanning the range from 7.3 to 2.3 mM, in line with the model predictions. An unexpected ancillary finding was the existence of a microdomain under the host cell membrane with reduced calcein quenching by hemoglobin in cells with mature trophozoite stage parasites.The results support the predictions of the colloid-osmotic hypothesis and provide a better understanding of the homeostasis of malaria-infected red cells. In addition, they revealed the existence of a distinct peripheral microdomain in the host cell with limited access to hemoglobin molecules indicating the

Quantifying risk of penile prosthesis infection with elevated glycosylated hemoglobin.

Science.gov (United States)

Wilson, S K; Carson, C C; Cleves, M A; Delk, J R

1998-05-01

Elevation of glycosylated hemoglobin above levels of 11.5 mg.% has been considered a contraindication to penile prosthesis implantation in diabetic patients. We determine the predictive value of glycosylated hemoglobin A1C in penile prosthesis infections in diabetic and nondiabetic patients to confirm or deny this prevalent opinion. We conducted a 2-year prospective study of 389 patients, including 114 diabetics, who underwent 3-piece penile prosthesis implantation. All patients had similar preoperative preparation without regard to diabetic status, control or glycosylated hemoglobin A1C level. Risk of infection was statistically analyzed for diabetics versus nondiabetics, glycosylated hemoglobin A1C values above and below 11.5 mg.%, insulin dependent versus oral medication diabetics, and fasting blood sugars above and below 180 mg.%. Prosthesis infections developed in 10 diabetics (8.7%) and 11 nondiabetics (4.0%). No increased infection rate was observed in diabetics with high fasting sugars or diabetics on insulin. There was no statistically significant increased infection risk with increased levels of glycosylated hemoglobin A1C among all patients or among only the diabetics. In fact, there was no meaningful difference in the median or mean level of glycosylated hemoglobin A1C in the infected and noninfected patients regardless of diabetes. Use of glycosylated hemoglobin A1C values to identify and exclude surgical candidates with increased risk of infections is not proved by this study. Elevation of fasting sugar or insulin dependence also does not increase risk of infection in diabetics undergoing prosthesis implantation.

PERBEDAAN KADAR HEMOGLOBIN METODE SIANMETHEMOGLOBIN DENGAN DAN TANPA SENTRIFUGASI PADA SAMPEL LEUKOSITOSIS

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wahdah norsiah

2015-12-01

Full Text Available Abstract: Examination of hemoglobin levels influenced leukocytosis sianmethemoglobin method that causes increased absorbance measurements of hemoglobin levels increased significantly and the false blood sample that has been diluted with a solution Drabkins in centrifugation at 3000 rpm for 10 minutes and then the absorbance of the supernatant was measured with a photometer at λ 546 nm. This study aimed to analyze the differences in hemoglobin level examination siamethemoglobin method with and without centrifugation at sample leukocytosis. This type of research is observational research laboratory. The study design was cross-sectional study. Samples were taken from the remaining blood samples of patients who have been examined leukositnya number more than 20,000 / uL with Hematology Analyzer (CEL-DYN Ruby February-April 2014, and were divided into 4 groups based on criteria that group 1. leukocyte count of 20,000 / uL-29 999 / mL, group II. 30,000 / uL-39 999 / uL, the group III. 40,000 / uL-49,999 / uL, the group IV. More than 50,000 / uL. The number of samples taken were 20 samples of each group, a total sample of 80 samples. The analysis showed no significant difference in hemoglobin levels siamethemoglobin method with and without centrifugation at sample leukocytosis with a value of p = 0.000 less than 0.05 α. Leukocytosis Turbidity affects the difference in hemoglobin levels with and without centrifugation, the higher the number the greater the difference in leukocyte levels of hemoglobin, hemoglobin level examination results of the study based on the criteria of the number of leukocytes obtained by the difference in hemoglobin levels with and without centrifugation in group I. 0.22 ± 0.07 g / dL, group II 0.40 ± 0.22 g / dL, a group III. 0.44 ± 0.14 g / dL, Group IV. 0.85 ± 0.41 g / dL. The level of hemoglobin in the sample sianmethemoglobin method leukocytosis with more than 20,000 / uL need a centrifuge so that appropriate

[Hemoglobin variants in Colombian patients referred to discard hemoglobinopathies].

Science.gov (United States)

Romero-Sánchez, Consuelo; Gómez Gutiérrez, Alberto; Duarte, Yurani; Amazo, Constanza; Manosalva, Clara; Chila M, Lorena; Casas-Gómez, María Consuelo; Briceño Balcázar, Ignacio

2015-10-01

Oxygen transport is altered in hemoglobinopathies. To study the distribution of hemoglobinopathies in Andean subjects without African ancestry. We analyzed blood samples of 1,407 subjects aged 18 to 59 years (58% females), living in the central Andean region of Colombia, referred to discard hemoglobinopathies. The frequency and type of hemoglobinopathy was established by capillary and agarose gel electrophoresis. The frequency of hemoglobinopathies was 34.5% and higher among females. The structural variants found were: AS-heterozygous hemoglobin (8.1%), homozygous SS (3.7%), heterozygous SC (2.2%), AC heterozygotes (0.5%) and heterozygous AE (0.3%). Quantitative variants found were Hb A-Beta thalassemia (13.91%) and Hb H (0.06%), Beta-thalassemia heterozygotes C (0.88%), S-Beta thalassemia heterozygotes (6.07%) and compound heterozygous SC/Beta thalassemia (0.25%), with a persistence of fetal hemoglobin 0. Composite thalassemia was also found in 31%. All techniques showed good correlation and capillary electrophoresis demonstrated a greater detection of hemoglobin variants. The frequency of hemoglobin variants in the analyzed population was high, which is an important public health indicator. The most common hemoglobin variant was HbA/Increased structural Hb A2 and the mos frequent structural hemoglobinopathy was sickle cell trait. Capillary electrophoresis can discern any Hb variants present in the population.

Study of LAXS Profile of Hemoglobin from Irradiated Blood

International Nuclear Information System (INIS)

Selim, N.S.; Desouky, O.S.; Elshemey, W.M.

2006-01-01

The present work aims to move a step forward towards a deeper understanding of the scattering of x-ray, from lyophilized biological samples. Comparative study has been performed using low angle x-ray scattering (LAXS) and UV-visible spectrophotometry for monitoring the dose response characteristics of the hemoglobin molecule of irradiated blood. Blood samples were exposed to gamma rays, at doses ranging from 5 up to 100 Gy. Diluted hemoglobin solution was scanned in the UV-visible range (200-700 nm), and lyophilized hemoglobin was prepared for LAXS measurement. The radiation-induced changes in the hemoglobin structure have been evaluated. The LAXS profile of hemoglobin molecule is characterized by the presence of 2 peaks in the forward direction of scattering. These peaks were found to be sensitive to the variations in the molecular structure of a given sample. The obtained results suggest that the 1st peak, recorded at 4.65O (equivalent to momentum transfer, x= 0.526 nm-1), is sensitive to the tertiary and quaternary structure of the globin part, while the major peak, recorded at 10.5O (equivalent to momentum transfer, x= 1.189 nm-1), appeared to be related to its primary and secondary structure

Is a blood sample for hemoglobins in the transfusional range reliable?

Science.gov (United States)

López, A; Gómez, L; Petinal, G; Adán, N; Alvarado, S; Carballo, N

2018-02-27

To evaluate the correlation and agreement in our unit and population of hemoglobin in gasometry versus hematology analyzer, to evaluate errors in transfusion or lack thereof. strong association between Point-of-care (POC) and hematimetry, with P<.001, with a coefficient of determination r 2 of 0.56, an intraclass correlation coefficient of 0.63 and a Lin's concordance correlation coefficient of 0.65. For hemoglobins less than 7g/dL, a success rate of 29.41% was obtained. Low-moderate agreement of POC hemoglobin with standard haemothymetry. High probability of errors in the indication of transfusion based on gasometer hemoglobins, especially in low hemoglobins. Copyright © 2018 Sociedad Española de Anestesiología, Reanimación y Terapéutica del Dolor. Publicado por Elsevier España, S.L.U. All rights reserved.

Hemoglobin Levels Across the Pediatric Critical Care Spectrum: A Point Prevalence Study.

Science.gov (United States)

Hassan, Nabil E; Reischman, Diann E; Fitzgerald, Robert K; Faustino, Edward Vincent S

2018-05-01

To determine the prevailing hemoglobin levels in PICU patients, and any potential correlates. Post hoc analysis of prospective multicenter observational data. Fifty-nine PICUs in seven countries. PICU patients on four specific days in 2012. None. Patients' hemoglobin and other clinical and institutional data. Two thousand three hundred eighty-nine patients with median age of 1.9 years (interquartile range, 0.3-9.8 yr), weight 11.5 kg (interquartile range, 5.4-29.6 kg), and preceding PICU stay of 4.0 days (interquartile range, 1.0-13.0 d). Their median hemoglobin was 11.0 g/dL (interquartile range, 9.6-12.5 g/dL). The prevalence of transfusion in the 24 hours preceding data collection was 14.2%. Neonates had the highest hemoglobin at 13.1 g/dL (interquartile range, 11.2-15.0 g/dL) compared with other age groups (p < 0.001). The percentage of 31.3 of the patients had hemoglobin of greater than or equal to 12 g/dL, and 1.1% had hemoglobin of less than 7 g/dL. Blacks had lower median hemoglobin (10.5; interquartile range, 9.3-12.1 g/dL) compared with whites (median, 11.1; interquartile range, 9.0-12.6; p < 0.001). Patients in Spain and Portugal had the highest median hemoglobin (11.4; interquartile range, 10.0-12.6) compared with other regions outside of the United States (p < 0.001), and the highest proportion (31.3%) of transfused patients compared with all regions (p < 0.001). Patients in cardiac PICUs had higher median hemoglobin than those in mixed PICUs or noncardiac PICUs (12.3, 11.0, and 10.6 g/dL, respectively; p < 0.001). Cyanotic heart disease patients had the highest median hemoglobin (12.6 g/dL; interquartile range, 11.1-14.5). Multivariable regression analysis within diagnosis groups revealed that hemoglobin levels were significantly associated with the geographic location and history of complex cardiac disease in most of the models. In children with cancer, none of the variables tested correlated with patients' hemoglobin levels

Ecole d’été de probabilités de Saint-Flour XXXII

CERN Document Server

2004-01-01

This is yet another indispensable volume for all probabilists and collectors of the Saint-Flour series, and is also of great interest for mathematical physicists. It contains two of the three lecture courses given at the 32nd Probability Summer School in Saint-Flour (July 7-24, 2002). Boris Tsirelson's lectures introduce the notion of nonclassical noise produced by very nonlinear functions of many independent random variables, for instance singular stochastic flows or oriented percolation. Two examples are examined (noise made by a Poisson snake, the Brownian web). A new framework for the scaling limit is proposed, as well as old and new results about noises, stability, and spectral measures. Wendelin Werner's contribution gives a survey of results on conformal invariance, scaling limits and properties of some two-dimensional random curves. It provides a definition and properties of the Schramm-Loewner evolutions, computations (probabilities, critical exponents), the relation with critical exponents of planar...

Osteonecrose da mandíbula associada aos bifosfonatos em doentes com cancro do pulmão

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Inês Neves

2013-09-01

Full Text Available Resumo: A osteonecrose da mandíbula (ONM pode surgir como complicação do tratamento com bifosfonatos. Esta patologia tem sido descrita em doentes com cancro e o seu desenvolvimento associado a exposição prolongada a altas doses de bifosfonatos. Má higiene dentária, história de uso de próteses ou extração dentária, quimioterapia, corticosteroides e radioterapia da cabeça e pescoço são fatores de risco reportados. Nas fases iniciais pode ser assintomática, contudo, o doente posteriormente desenvolve dor significativa e exposição óssea progressiva. Os autores descrevem 3 casos de ONM em doentes com cancro do pulmão após exposição prolongada a bifosfonatos e na presença de fatores de risco conhecidos. A ONM pode atingir seriamente a qualidade de vida dos doentes com cancro. O diagnóstico precoce poderá reduzir ou mesmo evitar as consequências da lesão óssea progressiva. Abstract: Osteonecrosis of the jaw (ONJ can occur as a complication of bisphosphonate therapy. This condition has been described in cancer patients and its development has been associated with prolonged exposure to high doses of bisphosphonates. Bad dental hygiene, a history of prosthesis or dental extraction, chemotherapy, corticosteroids, and radiation therapy of the head and neck are risk factors reported. In the initial stages it may be asymptomatic, but the patient subsequently develops severe pain and progressive exposed bone. The authors describe three cases of ONJ in lung cancer patients after prolonged exposure to bisphosphonates and there were known risk factors. ONJ can seriously affect the quality of life of cancer patients. An early diagnosis may reduce or avoid the consequences of progressive bone lesion. Palavras-chave: Osteonecrose da mandíbula associada aos bifosfonatos, Cancro do pulmão, Fatores de risco, Tratamento, Keywords: Bisphosphonate-associated osteonecrosis of the

Point-of-care hemoglobin testing for postmortem diagnosis of anemia.

Science.gov (United States)

Na, Joo-Young; Park, Ji Hye; Choi, Byung Ha; Kim, Hyung-Seok; Park, Jong-Tae

2018-03-01

An autopsy involves examination of a body using invasive methods such as dissection, and includes various tests using samples procured during dissection. During medicolegal autopsies, the blood carboxyhemoglobin concentration is commonly measured using the AVOXimeter® 4000 as a point-of-care test. When evaluating the body following hypovolemic shock, characteristics such as reduced livor mortis or an anemic appearance of the viscera can be identified, but these observations arequite subjective. Thus, a more objective test is required for the postmortem diagnosis of anemia. In the present study, the AVOXimeter® 4000 was used to investigate the utility of point-of-care hemoglobin testing. Hemoglobin tests were performed in 93 autopsy cases. The AVOXimeter® 4000 and the BC-2800 Auto Hematology Analyzer were used to test identical samples in 29 of these cases. The results of hemoglobin tests performed with these two devices were statistically similar (r = 0.969). The results of hemoglobin tests using postmortem blood were compared with antemortem test results from medical records from 31 cases, and these results were similar. In 13 of 17 cases of death from internal hemorrhage, hemoglobin levels were lower in the cardiac blood than in blood from the affected body cavity, likely due to compensatory changes induced by antemortem hemorrhage. It is concluded that blood hemoglobin testing may be useful as a point-of-care test for diagnosing postmortem anemia.

Enhancement of Salinity Tolerance during Rice Seed Germination by Presoaking with Hemoglobin

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Sheng Xu

2011-04-01

Full Text Available Salinity stress is an important environmental constraint limiting the productivity of many crops worldwide. In this report, experiments were conducted to investigate the effects of seed presoaking by bovine hemoglobin, an inducer of heme oxygenase-1 (HO-1, on salinity tolerance in rice (Oryza sativa plants. The results showed that different concentrations of the hemoglobin (0.01, 0.05, 0.2, 1.0, and 5.0 g/L differentially alleviated the inhibition of rice seed germination and thereafter seedling shoot growth caused by 100 mM NaCl stress, and the responses of 1.0 g/L hemoglobin was the most obvious. Further analyses showed that application of hemoglobin not only increased the HO-1 gene expression, but also differentially induced catalase (CAT, ascorbate peroxidase (APX, and superoxide dismutase (SOD activities or transcripts, thus decreasing the lipid peroxidation in germinating rice seeds subjected to salt stress. Compared with non-hemoglobin treatment, hemoglobin presoaking also increased the potassium (K to sodium (Na ratio both in the root and shoot parts after salinity stress. The effect is specific for HO-1 since the potent HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX blocked the positive actions of hemoglobin on seed germination and seedling shoot growth. Overall, these results suggested that hemoglobin performs an advantageous role in enhancement of salinity tolerance during rice seed germination.

Structural and functional properties of hemoglobins from unicellular organisms as revealed by resonance Raman spectroscopy.

Science.gov (United States)

Egawa, Tsuyoshi; Yeh, Syun-Ru

2005-01-01

Hemoglobins have been discovered in organisms from virtually all kingdoms. Their presence in unicellular organisms suggests that the gene for hemoglobin is very ancient and that the hemoglobins must have functions other than oxygen transport, in view of the fact that O2 delivery is a diffusion-controlled process in these organisms. Based on sequence alignment, three groups of hemoglobins have been characterized in unicellular organisms. The group-one hemoglobins, termed truncated hemoglobins, consist of proteins with 110-140 amino acid residues and a novel two-over-two alpha-helical sandwich motif. The group-two hemoglobins, termed flavohemoglobins, consist of a hemoglobin domain, with a classical three-over-three alpha-helical sandwich motif, and a flavin-containing reductase domain that is covalently attached to it. The group-three hemoglobins consist of myoglobin-like proteins that have high sequence homology and structural similarity to the hemoglobin domain of flavohemoglobins. In this review, recent resonance Raman studies of each group of these proteins are presented. Their implications are discussed in the context of the structural and functional properties of these novel hemoglobins.

Hemoglobin Rahere, a human hemoglobin variant with amino acid substitution at the 2,3-diphosphoglycerate binding site. Functional consequences of the alteration and effects of bezafibrate on the oxygen bindings.

Science.gov (United States)

Sugihara, J; Imamura, T; Nagafuchi, S; Bonaventura, J; Bonaventura, C; Cashon, R

1985-09-01

We encountered an abnormal hemoglobin (Rahere), with a threonine residue replacing the beta 82 (EF6) lysine residue at the binding site of 2,3-diphosphoglycerate, which was responsible for overt erythrocytosis in two individuals of a Japanese family. Hemoglobin Rahere shows a lower oxygen affinity on the binding of 2,3-diphosphoglycerate or chloride ions than hemoglobin A. Although a decrease in the positive charge density at the binding sites of 2,3-diphosphoglycerate in hemoglobin Rahere apparently shifts the allosteric equilibrium toward the low affinity state, it greatly diminishes the cofactor effects by anions. The oxygen affinity of the patient's erythrocytes is substantially lowered by the presence of bezafibrate, which combines with sites different from those of 2,3-diphosphoglycerate in either hemoglobin Rahere or hemoglobin A.

[The embroidery work of the lady at Saint-Anne Hospital].

Science.gov (United States)

Thillaud, Pierre L; Postel, Jacques

2014-01-01

In July 1974, a 72 old woman had been a patient for forty years in Sainte-Anne Hospital, Ward C. As she had again a violent brawl with her neighbour patient, she revealed being a tremendous artist. She had been confined on account of dementia paralytica in the Mecca of malariotherapy, and passionately devoted herself to embroidery. Her fancy work was rather a matter for Jean Dubuffet's art through its perfect expression and deserved being known.

L’entre-soi élitaire à Saint-Barthélemy

NARCIS (Netherlands)

Cousin, B.; Chauvin, S.

2012-01-01

Après un siècle de dénuement, l’île antillaise de Saint-Barthélemy a connu un développement continu depuis sa « découverte » par la grande bourgeoisie américaine à la fin des années 1950. Centrées autour de la villégiature de luxe, les activités économiques de l’île ont nécessité le recours

Hemoglobin system of Sparus aurata: Changes in fishes farmed under extreme conditions

International Nuclear Information System (INIS)

Campo, Salvatore; Nastasi, Giancarlo; D'Ascola, Angela; Campo, Giuseppe M.; Avenoso, Angela; Traina, Paola; Calatroni, Alberto; Burrascano, Emanuele; Ferlazzo, Alida; Lupidi, Giulio; Gabbianelli, Rosita; Falcioni, Giancarlo

2008-01-01

In order to gain more knowledge on the stress responses of gilhead seabream (Sparus aurata) under extreme conditions, this study investigated the functional properties of the hemoglobin system and globin gene expression under hypoxia and low salinity. The oxygen affinity for the two hemoglobin components present inside the S. aurata erythrocyte was practically identical as was the influence of protons and organic phosphates (Root effect). The quantification of S. aurata hemoglobin fractions performed by HPLC and the data on gene expression of globin chains assayed by PCR indicate that under hypoxia and low salinity there is a change in the ratio between the two different hemoglobin components. The result indicating that the distinct hemoglobins present in S. aurata erythrocyte have almost identical functional properties, does not explain the adaptive response (expression change) following exposure of the animal to hypoxia or low salinity on the basis of their function as oxygen transporter. We hypothesize that other parallel biological functions that the hemoglobin molecule is known to display within the erythrocyte are involved in adaptive molecular mechanisms. The autoxidation-reduction cycle of hemoglobin could be involved in the response to particular living conditions

Novel subunit structure observed for noncooperative hemoglobin from Urechis caupo.

Science.gov (United States)

Kolatkar, P R; Meador, W E; Stanfield, R L; Hackert, M L

1988-03-05

Tetrameric hemoglobin from the "fat innkeeper" worm Urechis caupo possesses a novel subunit arrangement having an "inside out" quaternary structure in that the G/H helices are located on the outer surface of the tetramer. A 5-A resolution crystal structure reveals that although the individual subunits are beta-like, having a distinct D helix and the general myoglobin fold, the subunit contacts are very different from those previously observed for hemoglobins. Furthermore, the hemoglobin from U. caupo is also quite different from the unusual hemoglobin tetramer from clam which also has its G/H helices on the outer surface but with the hemes in close proximity through E-F helical contacts (Royer, W. E., Jr., Love, W. E., and Fenderson, F. F. (1985) Nature 316, 277-280).

Hemoglobin as a factor in the control of tumor oxygenation

International Nuclear Information System (INIS)

Hirst, D.G.

1987-01-01

The concentration of hemoglobin in the blood has been shown to have a market effect on the radiosensitivity of human and animal tumors. Experimental studies in mice indicate that radiosensitivity is influenced by a change in the hemoglobin level rather than by the absolute concentration. This dependence may be exploited to therapeutic advantage. Recent studies of hemoglobin/oxygen affinity have shown that the concentration of 2,3 diphosphoglycerate (2,3 DPG) affects tumor sensitivity to X-rays. Increased 2,3 DPG levels increase radiosensitivity in several mouse tumors. The time dependence of this effect remains to be established. The effective application of these effects in man may depend on the development of drugs which produce changes in hemoglobin affinity without the need for blood transfusions. Several drugs are currently being investigated

Historical basis of the Miracula of Saint Symeon in Stefan the First-Crowned’s life of Symeon

Directory of Open Access Journals (Sweden)

Komatina Ivana

2014-01-01

Full Text Available The paper is devoted to the research of the so-called Catalog of Miracles in the Life of Saint Symeon by Stefan the First-Crowned. Because the miracles are divided in the Life into those in which Symeon-Nemanja is revealed as a Saint, and those in which his heir Stefan the First-Crowned conquers the enemies of the Serbian State with his help, the attention is paid on research of their historical basis and chronological frame, as well as their religious essence. [Projekat Ministarstva nauke Republike Srbije, br. 177029: Srednjovekovne srpske zemlje (13-15. vek: politički, privredni, društveni i pravni procesi

Síntese e caracterização de compostos diorganoestânicos com ácido dl-mandélico

OpenAIRE

TERRA, Vilma Reis; BARBIÉRI, Roberto Santos; CASTELO BRANCO, Pedro de Azevedo; ABRAS, Anuar

1998-01-01

Por reação de quantidades eqüimoleculares de R2SnO ou R2SnCl2 (R = -CH3; -C4H9) com ácido dl-mandélico, C6H5CH(OH)COOH, em meio de etanol, foram obtidos novos compostos formulados como [(LR2Sn)2O] [L= C6H5CH(OH)COO-], nos quais o átomo de estanho é pentacoordenado, com o ligante L estabelecendo uma ligação bidentada através dos átomos de oxigênio dos grupos ácido e hidroxila alcoólica. Nas mesmas condições, nenhum produto obtido a partir da reação de (C6H5)2SnO ou (C6H5)2SnCl2 pode ser identi...

Simultaneous estimation of transcutaneous bilirubin, hemoglobin, and melanin based on diffuse reflectance spectroscopy

Science.gov (United States)

Nishidate, Izumi; Abdul, Wares MD.; Ohtsu, Mizuki; Nakano, Kazuya; Haneishi, Hideaki

2018-02-01

We propose a method to estimate transcutaneous bilirubin, hemoglobin, and melanin based on the diffuse reflectance spectroscopy. In the proposed method, the Monte Carlo simulation-based multiple regression analysis for an absorbance spectrum in the visible wavelength region (460-590 nm) is used to specify the concentrations of bilirubin (Cbil), oxygenated hemoglobin (Coh), deoxygenated hemoglobin (Cdh), and melanin (Cm). Using the absorbance spectrum calculated from the measured diffuse reflectance spectrum as a response variable and the extinction coefficients of bilirubin, oxygenated hemoglobin, deoxygenated hemoglobin, and melanin, as predictor variables, multiple regression analysis provides regression coefficients. Concentrations of bilirubin, oxygenated hemoglobin, deoxygenated hemoglobin, and melanin, are then determined from the regression coefficients using conversion vectors that are numerically deduced in advance by the Monte Carlo simulations for light transport in skin. Total hemoglobin concentration (Cth) and tissue oxygen saturation (StO2) are simply calculated from the oxygenated hemoglobin and deoxygenated hemoglobin. In vivo animal experiments with bile duct ligation in rats demonstrated that the estimated Cbil is increased after ligation of bile duct and reaches to around 20 mg/dl at 72 h after the onset of the ligation, which corresponds to the reference value of Cbil measured by a commercially available transcutaneous bilirubin meter. We also performed in vivo experiments with rats while varying the fraction of inspired oxygen (FiO2). Coh and Cdh decreased and increased, respectively, as FiO2 decreased. Consequently, StO2 was dramatically decreased. The results in this study indicate potential of the method for simultaneous evaluation of multiple chromophores in skin tissue.

Site-specific semisynthetic variant of human hemoglobin

International Nuclear Information System (INIS)

Hefta, S.A.; Lyle, S.B.; Busch, M.R.; Harris, D.E.; Matthew, J.B.; Gurd, F.R.N.

1988-01-01

A single round of Edman degradation was employed to remove the NH 2 -terminal valine from isolated α chains of human hemoglobin. Reconstitution of normal β chains with truncated or substituted α chains was used to form truncated (des-Val 1 -α1) and substituted ([[1- 13 C]Gly 1 ]α1) tetrameric hemoglobin analogs. Structural homology of the analogs with untreated native hemoglobin was established by using several spectroscopic and physical methods. Functional studies indicate that the reconstituted tetrameric protein containing des-Val 1 -α chains has a higher affinity for oxygen, is less influenced by chloride ions or 2,3-biphosphoglycerate, and shows lower cooperativity than native hemoglobin. These results confirm the key functional role of the α-chain NH 2 terminus in mediating cooperative oxygen binding across the dimer interface. The NH 2 -terminal pK/sub 1/2/ value was determined for the [ 13 C]glycine-substituted analog to be 7.46 +/- 0.09 at 15 0 C in the carbon monoxide-liganded form. This value, measured directly by 13 C NMR, agrees with the determination made by the less-direct 13 CO 2 method and confirms the role of this residue as a contributor to the alkaline Bohr effect; however, it is consistent with the presence of an NH 2 -terminal salt bridge to the carboxylate of Arg-141 of the α chain in the liganded form

An annotated checklist of scale insects (Hemiptera: Coccoidea) of Saint Lucia, Lesser Antilles .

Science.gov (United States)

Malumphy, Chris

2014-07-31

An annotated list of 83 scale insect species (Hemiptera: Sterorrhyncha: Coccoidea) recorded from Saint Lucia is presented, based on data gathered from UK quarantine interceptions, samples collected in an urban coastal habitat in the North West of the Island in 2013, and published records. Thirty-three species (40%) are recorded for the first time for the country, including Dysmicoccus joannesiae (Costa Lima), a South American mealybug, and Poliaspoides formosana (Takahashi), an Asian armoured scale insect pest of bamboo, which are new for the Caribbean region. The economic, environmental and social impacts caused by introduced exotic species of scale insect are discussed. Two predatory midges Diadiplosis ?coccidivora (Felt) and Diadiplosis multifila (Felt) (Diptera: Cecidomyiidae) are recorded for the first time from Saint Lucia. The latter species was observed causing 90% mortality of a large infestation of passion vine mealybug Planococcus minor (Maskell) on soursop fruit. 

Improvements in or relating to antibodies active against human hemoglobin Asub(1C)

International Nuclear Information System (INIS)

Javid, J.; Cerami, A.; Koenig, R.J.; Pettis, P.K.

1980-01-01

A method is described for preparing an antibody against human hemoglobin Asub(1c) which is substantially free of cross-reactivity against the human hemoglobins A 0 , Asub(1a) and Asub(1b). The antibodies are collected from cats, goats or sheep following injections of purified hemoglobin Asub(1c) antigen since these animals do not naturally produce hemoglobin Asub(1c). A radioimmunoassay method is also described whereby these antibodies are used to determine the quantity of hemoglobin Asub(1c) in blood samples. This is a useful technique in the diagnosis of diabetes mellitus. (U.K.)

The narrow therapeutic window of glycated hemoglobin and assay variability.

Science.gov (United States)

Hosseini, S S; Bibler, I; Charles, M A

1999-12-01

Glycated hemoglobin is measured by a variety of assays, each of which has a unique normal level. Our purpose is to show that among the different assays available in the United States, using the same patient's blood sample, assay results may vary widely and may more or less easily achieve a glycated hemoglobin value within the normal range. The following assays were compared using the same patient's blood sample for each pair of assays: glycohemoglobin affinity assay (GHB Reader; Isolab, Akron, OH) versus gel electrophoresis assay (n = 76); Isolab versus ion capture assay (IMX; Abbott Laboratories, Irving, TX) (n = 57); monoclonal antibody assay (DCA2000; Bayer Diagnostics, Pittsburgh, PA) versus IMX (n = 100); and high-performance liquid chromatography (HPLC) assay (Bio-Rad Variant A1c; Bio-Rad Laboratories, Richmond, CA) versus IMX assay (n = 55). Our analyses indicate that a relative ranking can be established for the ease of achieving a normal glycated hemoglobin level. The ranking indicates that the most stringent or difficult assays for achieving a normal level are the Isolab and DCA2000 assays. The intermediate assays are the IMX and Bio-Rad Variant, and the easiest method for achieving a normal value is the gel electrophoresis assay. Our results indicate that various glycated hemoglobin assays vary widely and are associated with more or less difficulty for an individual patient to achieve a glycated hemoglobin level within the normal range. These results are especially significant with respect to (1) the clinically narrow therapeutic window of glycated hemoglobin values in type 1 diabetes to avoid rapidly advancing severe hypoglycemia rates and chronic microvascular complication rates, and (2) the glycated hemoglobin threshold for rapidly advancing macrovascular disease in both type 1 and type 2 patients.

Parametric study of the low-enriched uranium integrated Fort-Saint-Vrain element; comparative evaluation with the interacting tubular element

International Nuclear Information System (INIS)

Cerles, J.M.; Carvallo, G.; Vallepin, C.

1971-11-01

This paper presents a study of the influence of the different geometric and neutronic parameters on the calculation of the cycle with low-enriched uranium in a Fort-Saint-Vrain type brick. The study is divided in two parts: a stage of physics, essentially neutronics; an economical part where the costs are taken into account. At the level of studies of neutronics and costs, a parallel comparison is developed between the brick Fort-Saint-Vrain and the interacting tubular element, and even thorium. 6 refs. 29 figs [fr

Lower versus Higher Hemoglobin Threshold for Transfusion in Septic Shock

DEFF Research Database (Denmark)

Holst, Lars B; Haase, Nicolai; Wetterslev, Jørn

2014-01-01

BACKGROUND: Blood transfusions are frequently given to patients with septic shock. However, the benefits and harms of different hemoglobin thresholds for transfusion have not been established. METHODS: In this multicenter, parallel-group trial, we randomly assigned patients in the intensive care...... unit (ICU) who had septic shock and a hemoglobin concentration of 9 g per deciliter or less to receive 1 unit of leukoreduced red cells when the hemoglobin level was 7 g per deciliter or less (lower threshold) or when the level was 9 g per deciliter or less (higher threshold) during the ICU stay...... were similar in the two intervention groups. CONCLUSIONS: Among patients with septic shock, mortality at 90 days and rates of ischemic events and use of life support were similar among those assigned to blood transfusion at a higher hemoglobin threshold and those assigned to blood transfusion...

Hemoglobin Rahere, a human hemoglobin variant with amino acid substitution at the 2,3-diphosphoglycerate binding site. Functional consequences of the alteration and effects of bezafibrate on the oxygen bindings.

OpenAIRE

Sugihara, J; Imamura, T; Nagafuchi, S; Bonaventura, J; Bonaventura, C; Cashon, R

1985-01-01

We encountered an abnormal hemoglobin (Rahere), with a threonine residue replacing the beta 82 (EF6) lysine residue at the binding site of 2,3-diphosphoglycerate, which was responsible for overt erythrocytosis in two individuals of a Japanese family. Hemoglobin Rahere shows a lower oxygen affinity on the binding of 2,3-diphosphoglycerate or chloride ions than hemoglobin A. Although a decrease in the positive charge density at the binding sites of 2,3-diphosphoglycerate in hemoglobin Rahere ap...

Influence of hemoglobin on non-invasive optical bilirubin sensing

Science.gov (United States)

Jiang, Jingying; Gong, Qiliang; Zou, Da; Xu, Kexin

2012-03-01

Since the abnormal metabolism of bilirubin could lead to diseases in the human body, especially the jaundice which is harmful to neonates. Traditional invasive measurements are difficult to be accepted by people because of pain and infection. Therefore, the real-time and non-invasive measurement of bilirubin is of great significance. However, the accuracy of currently transcutaneous bilirubinometry(TcB) is generally not high enough, and affected by many factors in the human skin, mostly by hemoglobin. In this talk, absorption spectra of hemoglobin and bilirubin have been collected and analyzed, then the Partial Least Squares (PLS) models have been built. By analyzing and comparing the Correlation and Root Mean Square Error of Prediction(RMSEP), the results show that the Correlation of bilirubin solution model is larger than that of the mixture solution added with hemoglobin, and its RMSEP value is smaller than that of mixture solution. Therefore, hemoglobin has influences on the non-invasive optical bilirubin sensing. In next step, it is necessary to investigate how to eliminate the influence.

Further contributions to a new tectonic interpretation of the Sainte Victoire Mountain (Provence, France)

Energy Technology Data Exchange (ETDEWEB)

Ricour, J.; Monteau, R.; Laville, P.; Agryriadis, I.

2016-10-01

A new tectonic interpretation of the Sainte Victoire Mountain (north of Marseille, south eastern France) is proposed, principally invoking vertical movements and providing evidence which suggests an uplift which may be still active today in the western part. Some new observations confirm our hypothesis. (Author)

Hemoglobin levels and new-onset heart failure in the community

NARCIS (Netherlands)

Klip, IJsbrand T.; Postmus, Douwe; Voors, Adriaan A.; Brouwers, Frank P. J.; Gansevoort, Ron T.; Bakker, Stephan J. L.; Hillege, Hans L.; de Boer, Rudolf A.; van der Harst, Pim; van Gilst, Wiek H.; van Veldhuisen, Dirk J.; van der Meer, Peter

Background In established cardiovascular disease and heart failure (HF), low hemoglobin levels are associated with unfavorable outcome. Whether hemoglobin levels are associated with the development of new-onset HF in the population is unclear. This study sought to investigate the relationship

Diet of the oilfish Ruvettus pretiosus (Perciformes: Gempylidae in the Saint Peter and Saint Paul archipelago, Brazil

Directory of Open Access Journals (Sweden)

Danielle de Lima Viana

2012-06-01

Full Text Available Feeding aspects of the oilfish, Ruvettus pretiosus, were studied based on 360 stomachs of both male and female specimens caught off the Saint Peter and Saint Paul Archipelago. The total length of the specimens ranged from 52.4 cm to 189.0 cm. Of the 360 stomachs examined, 135 presented some food and 225 were empty. Thirty-four taxa were identified, represented by 16 fish, 17 cephalopods and 1 crustacean. The stabilization of the food items richness was attained at 35 food items and 104 stomachs, approximately. A remarkable predation upon the flying fish Cheilopogon cyanopterus was observed around SPSPA, directly related to the main reproductive period of this species in the area. The oilfish's food spectrum shows that the species feeds on a wide vertical range in the water column, catching prey items at the surface or in shallow waters, as well as epi-mesopelagic fish, in addition to mesopelagic cephalopods.Aspectos alimentares do peixe-prego, Ruvettus pretiosus, foram estudados com base nos estômagos de 360 espécimes, de ambos os sexos, capturados nos arredores do Arquipélago de São Pedro e São Paulo. Todos os exemplares foram capturados com linha de mão. O comprimento total dos exemplares variou de 52,4 cm a 189,0 cm. Dos 360 estômagos examinados, 135 apresentaram algum alimento e 225 estavam vazios. Trinta e quatro táxons foram identificados, representados por 16 peixes, 17 cefalópodes e um crustáceo. A estabilização da riqueza dos itens alimentares foi obtida com 35 itens e 104 estômagos. Uma predação notável sobre o peixe-voador Cheilopogon cyanopterus foi observada nas adjacências do ASPSP, diretamente relacionada com o principal período reprodutivo dessa espécie na área. O espectro alimentar do peixe-prego mostra que a espécie se alimenta em uma ampla faixa vertical na coluna d'agua, capturando desde presas de superfície ou águas rasas, a peixes epi-mesopelágicos, além de cefalópodes mesopelágicos.

Modeling hemoglobin at optical frequency using the unconditionally stable fundamental ADI-FDTD method.

Science.gov (United States)

Heh, Ding Yu; Tan, Eng Leong

2011-04-12

This paper presents the modeling of hemoglobin at optical frequency (250 nm - 1000 nm) using the unconditionally stable fundamental alternating-direction-implicit finite-difference time-domain (FADI-FDTD) method. An accurate model based on complex conjugate pole-residue pairs is proposed to model the complex permittivity of hemoglobin at optical frequency. Two hemoglobin concentrations at 15 g/dL and 33 g/dL are considered. The model is then incorporated into the FADI-FDTD method for solving electromagnetic problems involving interaction of light with hemoglobin. The computation of transmission and reflection coefficients of a half space hemoglobin medium using the FADI-FDTD validates the accuracy of our model and method. The specific absorption rate (SAR) distribution of human capillary at optical frequency is also shown. While maintaining accuracy, the unconditionally stable FADI-FDTD method exhibits high efficiency in modeling hemoglobin.

Optical wavelength selection for portable hemoglobin determination by near-infrared spectroscopy method

Science.gov (United States)

Tian, Han; Li, Ming; Wang, Yue; Sheng, Dinggao; Liu, Jun; Zhang, Linna

2017-11-01

Hemoglobin concentration is commonly used in clinical medicine to diagnose anemia, identify bleeding, and manage red blood cell transfusions. The golden standard method for determining hemoglobin concentration in blood requires reagent. Spectral methods were advantageous at fast and non-reagent measurement. However, model calibration with full spectrum is time-consuming. Moreover, it is necessary to use a few variables considering size and cost of instrumentation, especially for a portable biomedical instrument. This study presents different wavelength selection methods for optical wavelengths for total hemoglobin concentration determination in whole blood. The results showed that modelling using only two wavelengths combination (1143 nm, 1298 nm) can keep on the fine predictability with full spectrum. It appears that the proper selection of optical wavelengths can be more effective than using the whole spectra for determination hemoglobin in whole blood. We also discussed the influence of water absorptivity on the wavelength selection. This research provides valuable references for designing portable NIR instruments determining hemoglobin concentration, and may provide some experience for noninvasive hemoglobin measurement by NIR methods.

PLASMA PROTEIN AND HEMOGLOBIN PRODUCTION

Science.gov (United States)

Robscheit-Robbins, F. S.; Miller, L. L.; Whipple, G. H.

1947-01-01

Given healthy dogs fed abundant iron and protein-free or low protein diets with sustained anemia and hypoproteinemia, we can study the capacity of these animals to produce simultaneously new hemoglobin and plasma protein. Reserve stores of blood protein-building materials are measurably depleted and levels of 6 to 8 gm. per cent for hemoglobin and 4 to 5 gm. per cent for plasma protein can be maintained for weeks or months depending upon the intake of food proteins or amino acid mixtures. These dogs are very susceptible to infection and various poisons. Dogs tire of these diets and loss of appetite terminates many experiments. Under these conditions (double depletion) standard growth mixtures of essential amino acids are tested to show the response in blood protein output and urinary nitrogen balance. As a part of each tabulated experiment one of the essential amino acids is deleted from the complete growth mixture to compare such response with that of the whole mixture. Methionine, threonine, phenylalanine, and tryptophane when singly eliminated from the complete amino acid mixture do effect a sharp rise in urinary nitrogen. This loss of urinary nitrogen is corrected when the individual amino acid is replaced in the mixture. Histidine, lysine, and valine have a moderate influence upon urinary nitrogen balance toward nitrogen conservation. Leucine, isoleucine, and arginine have minimal or no effect upon urinary nitrogen balance when these individual amino acids are deleted from the complete growth mixture of amino acids during 3 to 4 week periods. Tryptophane and to a less extent phenylalanine and threonine when returned to the amino acid mixture are associated with a conspicuous preponderance of plasma protein output over the hemoglobin output (Table 4). Arginine, lysine, and histidine when returned to the amino acid mixture are associated with a large preponderance of hemoglobin output. Various amino acid mixtures under these conditions may give a positive

Centro Federal de Comunidad Saint Louis - EE.UU.

Directory of Open Access Journals (Sweden)

Hellmuth, George

1980-04-01

Full Text Available The Community Federal Center lies in the outskirts of the town of Saint Louis. It was conceived and designed by the project authors — in a style characteristic of them — as a suburban architectonic complex for office use. The work, standing out over an open stretch of land, has an ample base, with a volume that is neither the actual tower of the city centre nor the classic low rural construction. The building's architectonic language permits an integration, by sympathy, in the context of the neighbouring uni-family housing zone, without naturally being assimilated in them, due to the disparity of functions. It is achieved through a fracture of scaled volumes, breaking the classic coldness of the big office building, whilst at the same time thanks to its robustness, it indicates the activity housed inside.

El Community Federal Center está emplazado en las afueras de la ciudad de Saint Louis. Fue concebido y diseñado por los autores del proyecto —en un estilo que les es característico—, como un complejo arquitectónico suburbano destinado a oficinas. La obra, que se eleva sobre un terreno despejado, es amplía de base, presentando un volumen que no es ni la torre propia del centro urbano ni la clásica construcción rural de baja altura. El lenguaje arquitectónico del edificio permite una integración, por simpatía, en el contexto de la zona de viviendas unifamiliares cercana, sin llegar, naturalmente, a asimilarse a ellas dada la disparidad de funciones. Se lleva a cabo por medio de una fractura de volúmenes, escalonados, que rompen la frialdad clásica del gran edificio de oficinas, sin dejar de indicar, por su robustez, la actividad que alberga en su interior.

Useful Brazilian plants listed in the field books of the French naturalist Auguste de Saint-Hilaire (1779-1853).

Science.gov (United States)

Brandão, Maria G L; Pignal, Marc; Romaniuc, Sergio; Grael, Cristiane F F; Fagg, Christopher W

2012-09-28

Information regarding the use of beneficial, native Brazilian plants was compiled by European naturalists in the 19th century. The French botanist Auguste de Saint-Hilaire (1779-1853) was one of the most important such naturalists; however, his manuscripts (field books) have not yet been studied, especially in the context of useful plants. To present data documented by Saint-Hilaire in his field book regarding the use of native plants by the Brazilians. Data on useful plants were obtained from field books (six volumes) deposited in the Muséum national d' Histoire naturelle in Paris, France. The vernacular names of the plants, registered as "N.V." or "Nom Vulg." in the field book, were carefully searched. Traditional information about these plants was translated and organised using a computer. The botanical identification of each plant was determined and updated from the original descriptions and names cited in the field books by A. de Saint-Hilaire. Correlated pharmacological studies were obtained from PubMed. A total of 283 useful plants were recorded from the field books and 165 (58.3%) could be identified to genus or species. Fifty-eight different traditional uses were registered for the identified plants; the most common were as purgatives and febrifuges. Other data recovered were related to edible fruits and plants with interesting sensorial characteristics. For the few species that have been subjected to laboratory studies, the efficacy of the recorded traditional uses was confirmed. The data recorded by the French naturalist A. de Saint-Hilaire represent a rich, unexplored source of information regarding the traditional uses of Brazilian plants. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

An analysis of postoperative hemoglobin levels in patients with a fractured neck of femur.

Science.gov (United States)

Nagra, Navraj S; van Popta, Dmitri; Whiteside, Sigrid; Holt, Edward M

2016-10-01

The aim of this study was to analyze the changes in hemoglobin level and to determine a suitable timeline for post-operative hemoglobin monitoring in patients undergoing fixation of femoral neck fracture. Patients who underwent either dynamic hip screw (DHS) fixation (n = 74, mean age: 80 years) or hip hemiarthroplasty (n = 104, mean age: 84 years) for femoral neck fracture were included into the study. The hemoglobin level of the patients was monitored perioperatively. Analysis found a statistically and clinically significant mean drop in hemoglobin of 31.1 g/L over time from pre-operatively (D0) to day-5 post-operatively (p hemoglobin values over hemiarthroplasty patients (p = 0.046). The decrease in hemoglobin in the first 24-h post-operative period (D0 to day-1) is an underestimation of the ultimate lowest value in hemoglobin found at day-2. Relying on the day-1 hemoglobin could be detrimental to patient care. We propose a method of predicting patients likely to be transfused, and recommend a protocol for patients undergoing femoral neck fracture surgery to standardize postoperative hemoglobin monitoring. Level IV Prognostic study. Copyright © 2016 Turkish Association of Orthopaedics and Traumatology. Production and hosting by Elsevier B.V. All rights reserved.

Nitric Oxide in Plants: The Roles of Ascorbate and Hemoglobin

Science.gov (United States)

Wang, Xiaoguang; Hargrove, Mark S.

2013-01-01

Ascorbic acid and hemoglobins have been linked to nitric oxide metabolism in plants. It has been hypothesized that ascorbic acid directly reduces plant hemoglobin in support of NO scavenging, producing nitrate and monodehydroascorbate. In this scenario, monodehydroascorbate reductase uses NADH to reduce monodehydroascorbate back to ascorbate to sustain the cycle. To test this hypothesis, rates of rice nonsymbiotic hemoglobin reduction by ascorbate were measured directly, in the presence and absence of purified rice monodehydroascorbate reductase and NADH. Solution NO scavenging was also measured methodically in the presence and absence of rice nonsymbiotic hemoglobin and monodehydroascorbate reductase, under hypoxic and normoxic conditions, in an effort to gauge the likelihood of these proteins affecting NO metabolism in plant tissues. Our results indicate that ascorbic acid slowly reduces rice nonsymbiotic hemoglobin at a rate identical to myoglobin reduction. The product of the reaction is monodehydroascorbate, which can be efficiently reduced back to ascorbate in the presence of monodehydroascorbate reductase and NADH. However, our NO scavenging results suggest that the direct reduction of plant hemoglobin by ascorbic acid is unlikely to serve as a significant factor in NO metabolism, even in the presence of monodehydroascorbate reductase. Finally, the possibility that the direct reaction of nitrite/nitrous acid and ascorbic acid produces NO was measured at various pH values mimicking hypoxic plant cells. Our results suggest that this reaction is a likely source of NO as the plant cell pH drops below 7, and as nitrite concentrations rise to mM levels during hypoxia. PMID:24376554

Evaluation of non cyanide methods for hemoglobin estimation

Directory of Open Access Journals (Sweden)

Vinaya B Shah

2011-01-01

Full Text Available Background: The hemoglobincyanide method (HiCN method for measuring hemoglobin is used extensively worldwide; its advantages are the ready availability of a stable and internationally accepted reference standard calibrator. However, its use may create a problem, as the waste disposal of large volumes of reagent containing cyanide constitutes a potential toxic hazard. Aims and Objective: As an alternative to drabkin`s method of Hb estimation, we attempted to estimate hemoglobin by other non-cyanide methods: alkaline hematin detergent (AHD-575 using Triton X-100 as lyser and alkaline- borax method using quarternary ammonium detergents as lyser. Materials and Methods: The hemoglobin (Hb results on 200 samples of varying Hb concentrations obtained by these two cyanide free methods were compared with a cyanmethemoglobin method on a colorimeter which is light emitting diode (LED based. Hemoglobin was also estimated in one hundred blood donors and 25 blood samples of infants and compared by these methods. Statistical analysis used was Pearson`s correlation coefficient. Results: The response of the non cyanide method is linear for serially diluted blood samples over the Hb concentration range from 3gm/dl -20 gm/dl. The non cyanide methods has a precision of + 0.25g/dl (coefficient of variation= (2.34% and is suitable for use with fixed wavelength or with colorimeters at wavelength- 530 nm and 580 nm. Correlation of these two methods was excellent (r=0.98. The evaluation has shown it to be as reliable and reproducible as HiCN for measuring hemoglobin at all concentrations. The reagents used in non cyanide methods are non-biohazardous and did not affect the reliability of data determination and also the cost was less than HiCN method. Conclusions: Thus, non cyanide methods of Hb estimation offer possibility of safe and quality Hb estimation and should prove useful for routine laboratory use. Non cyanide methods is easily incorporated in hemobloginometers

Interrater reliability of the Saint-Anne Dargassies Scale in assessing the neurological patterns of healthy preterm newborns

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Carla Ismirna Santos Alves

Full Text Available Abstract Objectives: to assess the interrater reliability of the Saint-Anne Dargassies Scale in assessing neurological patterns of healthy preterm newborns. Methods: twenty preterm newborns met the inclusion criteria for participation in this prospective study. The neurologic examination was performed using the Saint-Anne Dargassies Scale, showing normal serial cranial ultrasound examination. In order to test the reliability, the study was structured as follows: group I (rater 1/physiotherapist; rater 2/neonatologist; group II (rater 3/physiotherapist; rater 4/child neurologist and the gold standard (expert and professor in pediatric neurology. Results: high interrater agreement was observed between groups I - II compared with the gold standard in assessing postural pattern (p<0.01. Regarding the assessment ofprimitive reflexes, greater agreement was observed in the evaluation of palmar grasp reflex and Moro reflex (p< 0.01 for group I compared with the gold standard. An analysis of tone demonstrated heterogeneous agreement, without compromising the reliability of the scale. The probability of equality between measurements of head circumference in the two groups, compared with the gold standard, was observed. Conclusions: the Saint-Anne Dargassies Scale demonstrated high reliability and homogeneity with significant power of reproducibility and may be capable to identify preterm newborns suspected of having neurological deficits.

Megalin and cubilin are endocytic receptors involved in renal clearance of hemoglobin

DEFF Research Database (Denmark)

Gburek, Jakub; Verroust, Pierre J; Willnow, Thomas E

2002-01-01

-Sepharose affinity chromatography of solubilized renal brush-border membranes. Apparent dissociation constants of 1.7 microM for megalin and 4.1 microM for cubilin were determined by surface plasmon resonance analysis. The binding was calcium dependent in both cases. Uptake of fluorescence-labeled hemoglobin by BN......The kidney is the main site of hemoglobin clearance and degradation in conditions of severe hemolysis. Herein it is reported that megalin and cubilin, two epithelial endocytic receptors, mediate the uptake of hemoglobin in renal proximal tubules. Both receptors were purified by use of hemoglobin...... not affect the uptake. By use of immunohistochemistry, it was demonstrated that uptake of hemoglobin in proximal tubules of rat, mouse, and dog kidneys occurs under physiologic conditions. Studies on normal and megalin knockout mouse kidney sections showed that megalin is responsible for physiologic...

Role of Reversible Histidine Coordination in Hydroxylamine Reduction by Plant Hemoglobins (Phytoglobins).

Science.gov (United States)

Athwal, Navjot Singh; Alagurajan, Jagannathan; Andreotti, Amy H; Hargrove, Mark S

2016-10-18

Reduction of hydroxylamine to ammonium by phytoglobin, a plant hexacoordinate hemoglobin, is much faster than that of other hexacoordinate hemoglobins or pentacoordinate hemoglobins such as myoglobin, leghemoglobin, and red blood cell hemoglobin. The reason for differences in reactivity is not known but could be intermolecular electron transfer between protein molecules in support of the required two-electron reduction, hydroxylamine binding, or active site architecture favoring the reaction. Experiments were conducted with phytoglobins from rice, tomato, and soybean along with human neuroglobin and soybean leghemoglobin that reveal hydroxylamine binding as the rate-limiting step. For hexacoordinate hemoglobins, binding is limited by the dissociation rate constant for the distal histidine, while leghemoglobin is limited by an intrinsically low affinity for hydroxylamine. When the distal histidine is removed from rice phytoglobin, a hydroxylamine-bound intermediate is formed and the reaction rate is diminished, indicating that the distal histidine imidazole side chain is critical for the reaction, albeit not for electron transfer but rather for direct interaction with the substrate. Together, these results demonstrate that phytoglobins are superior at hydroxylamine reduction because they have distal histidine coordination affinity constants near 1, and facile rate constants for binding and dissociation of the histidine side chain. Hexacoordinate hemoglobins such as neuroglobin are limited by tighter histidine coordination that blocks hydroxylamine binding, and pentacoordinate hemoglobins have intrinsically lower hydroxylamine affinities.

Placental morphology at different maternal hemoglobin levels: a histopathological study

International Nuclear Information System (INIS)

Kiran, N.; Zubair, A.; Malik, T.M.

2015-01-01

To evaluate the histopathological parameters of the placenta like weight, infarct and syncytial knots, at different maternal hemoglobin levels, in both qualitative and quantitative manner. Study design: Descriptive study Place and Duration of Study: Army Medical College, National University of Sciences and Technology in collaboration with Department of Obstetrics and Gynecology, Military Hospital, Rawalpindi, Pakistan, from December 2011 to November 2012. Patients and Methods: A total of 75 placentas were included, that were collected from full term mothers at the time of childbirth. Placental weight was taken without umbilical cord and gross placental infarcts were noted. Samples of placental tissue were taken and stained by haematoxylin and eosin (H and E). Microscopic study was done to evaluate placental infarcts and syncytial knots. Results: Mean placental weight at normal and low maternal hemoglobin was 581.67 ± 83.97g and 482.58 ± 104.74g respectively. Gross placental infarcts were found in all cases having low maternal hemoglobin concentration (60% cases). Syncytial knots were found in all placentas but they were considerably more at decreasing levels of maternal hemoglobin (19.79 ± 5.22). Conclusion: The present study showed decrease in placental weight, increase in placental infarcts and syncytial knot hyperplasia at low maternal hemoglobin concentration, displaying adaptive alterations. (author)

The refractive index of human hemoglobin in the visible range

International Nuclear Information System (INIS)

Zhernovaya, O; Tuchin, V; Sydoruk, O; Douplik, A

2011-01-01

Because the refractive index of hemoglobin in the visible range is sensitive to the hemoglobin concentration, optical investigations of hemoglobin are important for medical diagnostics and treatment. Direct measurements of the refractive index are, however, challenging; few such measurements have previously been reported, especially in a wide wavelength range. We directly measured the refractive index of human deoxygenated and oxygenated hemoglobin for nine wavelengths between 400 and 700 nm for the hemoglobin concentrations up to 140 g l -1 . This paper analyzes the results and suggests a set of model functions to calculate the refractive index depending on the concentration. At all wavelengths, the measured values of the refractive index depended on the concentration linearly. Analyzing the slope of the lines, we determined the specific refraction increments, derived a set of model functions for the refractive index depending on the concentration, and compared our results with those available in the literature. Based on the model functions, we further calculated the refractive index at the physiological concentration within the erythrocytes of 320 g l -1 . The results can be used to calculate the refractive index in the visible range for arbitrary concentrations provided that the refractive indices depend on the concentration linearly.

Current hemoglobin levels are more predictive of disease progression than hemoglobin measured at baseline in patients receiving antiretroviral treatment for HIV type 1 infection

DEFF Research Database (Denmark)

Kowalska, Justyna D; Mocroft, Amanda; Blaxhult, Anders

2007-01-01

The role of hemoglobin levels as an independent prognostic marker of progression to AIDS and/or death in HIV-infected patients starting combination antiretroviral therapy (cART) was investigated. A total of 2,579 patients from the EuroSIDA cohort with hemoglobin, CD4 cell count, and HIV RNA viral...

A new polyethyleneglycol-derivatized hemoglobin derivative with decreased oxygen affinity and limited toxicity.

Science.gov (United States)

Zolog, Oana; Mot, Augustin; Deac, Florina; Roman, Alina; Fischer-Fodor, Eva; Silaghi-Dumitrescu, Radu

2011-01-01

A new protocol is described for derivatization of hemoglobin with polyethyleneglycol (PEG) via reaction of the unmodified native hemoglobin with an activated amine-reacting polyethylene glycol derivative which, unlike protocols previously described, leads to formation of a peptide bond between hemoglobin and PEG. Dioxygen binding and peroxide reactivities of the derivatized hemoglobin are examined, and found to be within reasonable limits, with the particular observation that, unlike with a few other derivatization protocols, the dioxygen affinity is slightly lower than that of native Hb. In cell culture tests (human umbilical vein epithelial cells, HUVEC), the derivatization protocol induces no toxic effect. These results show promise towards applicability for production of hemoglobin-based blood substitutes.

History of psychosurgery at Sainte-Anne Hospital, Paris, France, through translational interactions between psychiatrists and neurosurgeons.

Science.gov (United States)

Zanello, Marc; Pallud, Johan; Baup, Nicolas; Peeters, Sophie; Turak, Baris; Krebs, Marie Odile; Oppenheim, Catherine; Gaillard, Raphael; Devaux, Bertrand

2017-09-01

Sainte-Anne Hospital is the largest psychiatric hospital in Paris. Its long and fascinating history began in the 18th century. In 1952, it was at Sainte-Anne Hospital that Jean Delay and Pierre Deniker used the first neuroleptic, chlorpromazine, to cure psychiatric patients, putting an end to the expansion of psychosurgery. The Department of Neuro-psychosurgery was created in 1941. The works of successive heads of the Neurosurgery Department at Sainte-Anne Hospital summarized the history of psychosurgery in France. Pierre Puech defined psychosurgery as the necessary cooperation between neurosurgeons and psychiatrists to treat the conditions causing psychiatric symptoms, from brain tumors to mental health disorders. He reported the results of his series of 369 cases and underlined the necessity for proper follow-up and postoperative re-education, illustrating the relative caution of French neurosurgeons concerning psychosurgery. Marcel David and his assistants tried to follow their patients closely postoperatively; this resulted in numerous publications with significant follow-up and conclusions. As early as 1955, David reported intellectual degradation 2 years after prefrontal leucotomies. Jean Talairach, a psychiatrist who eventually trained as a neurosurgeon, was the first to describe anterior capsulotomy in 1949. He operated in several hospitals outside of Paris, including the Sarthe Psychiatric Hospital and the Public Institution of Mental Health in the Lille region. He developed stereotactic surgery, notably stereo-electroencephalography, for epilepsy surgery but also to treat psychiatric patients using stereotactic lesioning with radiofrequency ablation or radioactive seeds of yttrium-90. The evolution of functional neurosurgery has been marked by the development of deep brain stimulation, in particular for obsessive-compulsive disorder, replacing the former lesional stereotactic procedures. The history of Sainte-Anne Hospital's Neurosurgery Department sheds

Haptoglobin radioassay based on binding to solid-phase hemoglobin

International Nuclear Information System (INIS)

Hooper, D.C.; Reed, R.A.; Peacock, A.C.

1979-01-01

A specific and sensitive assay for haptoglobin based on binding to an easily prepred Sepharose-bound hemoglobin reagent is described. The assay is suitable for directly determining radiolabeled amino acid incorporation into haptoglobin in several liver cell systems in vitro and can be adapted to measure unlabeled free haptoglobin in plasma samples regardlss of the presence of the haptoglobin--hemoglobin complex

High Leptospira seroprevalence in captive and wild-caught vervet monkeys ( Chlorocebus sabeus) on the Caribbean island of Saint Kitts.

Science.gov (United States)

Rajeev, Sreekumari; Conan, Anne; Pratt, Nicola; Beierschmitt, Amy; Palmour, Roberta

2017-11-01

Leptospirosis is a zoonotic disease of global importance. Very little information is available on Leptospira infection in nonhuman primates. We report herein a high seroprevalence (49.4%; 95% confidence interval: 41.6-57.2%) to Leptospira serovars in vervet monkeys ( Chlorocebus sabeus) on the Caribbean island of Saint Kitts. Monkeys bred in captivity ( n = 81) had a significantly higher seroprevalence compared to wild-caught monkeys ( n = 81; p Leptospira serovars and seroconversion occurs in wild and captive vervet monkeys on the Caribbean island of Saint Kitts. Further studies are warranted to better understand epidemiology, transmission, pathology, and possible reservoir status in this species.

Baldrige Award cites two hospitals. Baptist, Saint Luke's hospitals honored for quality, performance.

Science.gov (United States)

Rees, Tom

2004-01-01

Baptist Hospital Inc., Pensacola, Fla.; and Saint Luke's Hospital, Kansas City, Mo., have received the prestigious Malcolm Baldrige National Quality Award in the category of healthcare. Named for a former secretary of commerce, the award recognizes efficiency, effectiveness and excellence. The two hospitals are among only seven companies in the U.S. to be so recognized this year.

Genetic and developmental variation of hemoglobin in the deermouse, Peromyscus maniculatus.

Science.gov (United States)

Maybank, K M; Dawson, W D

1976-04-01

A genetic investigation of electrophoretic hemoglobin variants of the deermouse, Peromyscus maniculatus, shows three alleles, Hblf, Hblr, and Hblo, at a duplicated site controlling the six adult phenotypes. The Hblf allele has not been described previously. The hemoglobin locus is not closely linked to the albino locus. Fetal hemoglobin is distinct from any of the adult components and has a slower electrophoretic mobility. The fetal phenotype changes to the adult type between the days 15 and 18 of prenatal life.

New optical museum at Saint-Petersburg for education and training

Science.gov (United States)

Vasil'ev, V. N.; Stafeef, S. K.; Tomilin, M. G.

2009-06-01

Nowadays the educational problem of teaching optics and photonics is to attract the young generation to the wonderful and magic world of light, optical science, technology and systems. The main issue is to explain that in the course of last several hundred years optics has been representing the most clear world view for humanity. In fact, the optics itself is a multidisciplinary complex of independent scientific directions, and, moreover, it has always been a generator of new fields of knowledge. Besides, optics and photonics are the fields within which the most fundamental problems of today's reality are to be resolved. It is absolutely necessary to encourage our scholars in getting optics and photonics education as an alternative physical basis to gaining solely computer knowledge. The main obstacle is the poor connection between program of optical education and the real optical researches, disintegration of different branches of the optical science, the demographic situation, some problems with teaching mathematics and physics at schools, and the collision between traditional educational methods and the mentality of the new generation. In Russia the Saint-Petersburg State University of Information Technologies, Mechanics and Optics offers partial solution to these problems: the organization of a real place for interactive optical science in a form of a new museum of optics, intended for education and training, seems to be the most effective way. This was the main reason for establishing such a museum in Saint-Petersburg at the end of 2008.

Hemoglobin is essential for normal growth of Arabidopsis organs

DEFF Research Database (Denmark)

Hebelstrup, Kim Henrik; Hunt, Peter; Dennis, Elizabeth

2006-01-01

In Arabidopsis thaliana, the class I hemoglobin AHb1 is transiently expressed in the hydathodes of leaves and in floral buds from young inflorescences. Nitric oxide (NO) accumulates to high levels in these organs when AHb1 is silenced, indicating an important role in metabolizing NO. AHb1-silence...... suggests that 3-on-3 hemoglobins apart from a role in hypoxic stress play a general role under non-stressed conditions where they are essential for normal development by controlling the level of NO which tends to accumulate in floral buds and leaf hydathodes of plants......In Arabidopsis thaliana, the class I hemoglobin AHb1 is transiently expressed in the hydathodes of leaves and in floral buds from young inflorescences. Nitric oxide (NO) accumulates to high levels in these organs when AHb1 is silenced, indicating an important role in metabolizing NO. AHb1-silenced...... lines are viable but show a mutant phenotype affecting the regions where AHb1 is expressed. Arabidopsis lines with an insertional knockout or overexpression of AHb2, a class II 3-on-3 hemoglobin, were generated. Seedlings overexpressing AHb2 show enhanced survival of hypoxic stress. The AHb2 knockout...

Motifs et manifestations de l’amour de l’homme pour Dieu et l’amour du prochain selon Saint Basile le Grand

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Ernest Janczyk

2017-04-01

Full Text Available This article addresses the issue of the motivation of the fundamental order of love for God and the neighbour which Saint Basil the Great used in his doctrine. Based on an analysis of the texts of Saint Basil, this article demonstrates the intimate relationship that should exist between the love for God and the love for man. The ideal of this relationship is represented, according to the analysis, by Jesus Christ, the only Saviour of the world.

Aspartic protease activities of schistosomes cleave mammalian hemoglobins in a host-specific manner

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Jeffrey W Koehler

2007-02-01

Full Text Available We examined the efficiency of digestion of hemoglobin from four mammalian species, human, cow, sheep, and horse by acidic extracts of mixed sex adults of Schistosoma japonicum and S. mansoni. Activity ascribable to aspartic protease(s from S. japonicum and S. mansoni cleaved human hemoglobin. In addition, aspartic protease activities from S. japonicum cleaved hemoglobin from bovine, sheep, and horse blood more efficiently than did the activity from extracts of S. mansoni. These findings support the hypothesis that substrate specificity of hemoglobin-degrading proteases employed by blood feeding helminth parasites influences parasite host species range; differences in amino acid sequences in key sites of the parasite proteases interact less or more efficiently with the hemoglobins of permissive or non-permissive hosts.

A microfluidic approach for hemoglobin detection in whole blood

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Nikita Taparia

2017-10-01

Full Text Available Diagnosis of anemia relies on the detection of hemoglobin levels in a blood sample. Conventional blood analyzers are not readily available in most low-resource regions where anemia is prevalent, so detection methods that are low-cost and point-of-care are needed. Here, we present a microfluidic approach to measure hemoglobin concentration in a sample of whole blood. Unlike conventional approaches, our microfluidic approach does not require hemolysis. We detect the level of hemoglobin in a blood sample optically by illuminating the blood in a microfluidic channel at a peak wavelength of 540 nm and measuring its absorbance using a CMOS sensor coupled with a lens to magnify the image onto the detector. We compare measurements in microchannels with channel heights of 50 and 115 μm and found the channel with the 50 μm height provided a better range of detection. Since we use whole blood and not lysed blood, we fit our data to an absorption model that includes optical scattering in order to obtain a calibration curve for our system. Based on this calibration curve and data collected, we can measure hemoglobin concentration within 1 g/dL for severe cases of anemia. In addition, we measured optical density for blood flowing at a shear rate of 500 s-1 and observed it did not affect the nonlinear model. With this method, we provide an approach that uses microfluidic detection of hemoglobin levels that can be integrated with other microfluidic approaches for blood analysis.

A microfluidic approach for hemoglobin detection in whole blood

Science.gov (United States)

Taparia, Nikita; Platten, Kimsey C.; Anderson, Kristin B.; Sniadecki, Nathan J.

2017-10-01

Diagnosis of anemia relies on the detection of hemoglobin levels in a blood sample. Conventional blood analyzers are not readily available in most low-resource regions where anemia is prevalent, so detection methods that are low-cost and point-of-care are needed. Here, we present a microfluidic approach to measure hemoglobin concentration in a sample of whole blood. Unlike conventional approaches, our microfluidic approach does not require hemolysis. We detect the level of hemoglobin in a blood sample optically by illuminating the blood in a microfluidic channel at a peak wavelength of 540 nm and measuring its absorbance using a CMOS sensor coupled with a lens to magnify the image onto the detector. We compare measurements in microchannels with channel heights of 50 and 115 μm and found the channel with the 50 μm height provided a better range of detection. Since we use whole blood and not lysed blood, we fit our data to an absorption model that includes optical scattering in order to obtain a calibration curve for our system. Based on this calibration curve and data collected, we can measure hemoglobin concentration within 1 g/dL for severe cases of anemia. In addition, we measured optical density for blood flowing at a shear rate of 500 s-1 and observed it did not affect the nonlinear model. With this method, we provide an approach that uses microfluidic detection of hemoglobin levels that can be integrated with other microfluidic approaches for blood analysis.

La estructura del poder en la "Mémoires" de Saint-Simon

OpenAIRE

Robles Vázquez, Carmen

2004-01-01

Análisis de las diferentes formas en que Saint-Simon presenta en sus Memorias el poder, y de qué manera se manifiesta éste en sus aspectos económicos, políticos, religiosos, familiares y sociales, tanto durante los últimos años del reinado de Luis XIV como durante la regencia de Felipe de Orleáns en una sociedad teocrática y absolutista, fuertemente jerarquizada, característica del Antiguo Régimen.

Concurrent measurement of cellular turbidity and hemoglobin to evaluate the antioxidant activity of plants.

Science.gov (United States)

Bellik, Yuva; Iguer-Ouada, Mokrane

2016-01-01

In past decades, a multitude of analytical methods for measuring antioxidant activity of plant extracts has been developed. However, when using methods to determine hemoglobin released from human erythrocytes treated with ginger extracts, we found hemoglobin concentrations were significantly higher than in untreated control samples. This suggests in the presence of antioxidants that measuring hemoglobin alone is not sufficient to determine hemolysis. We show concurrent measurement of erythrocyte concentration and hemoglobin is essential in such assays, and describe a new protocol based on simultaneous measurement of cellular turbidity and hemoglobin. Copyright © 2015 Elsevier Ltd. All rights reserved.

Fratura de mandíbula: análise de 293 pacientes tratados no Hospital de Clínicas da Universidade Federal de Uberlândia Mandibular fracture: analysis of 293 patients treated in the Hospital of Clinics, Federal University of Uberlândia

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Lucas Gomes Patrocínio

2005-10-01

Full Text Available A fratura de mandíbula ocupa o segundo lugar entre as fraturas dos ossos da face, tendo havido aumento significativo de casos nos últimos anos. A não-identificação e o tratamento inadequado podem levar à deformidade estética ou funcional permanente. OBJETIVO: Avaliar os casos submetidos à redução de fratura de mandíbula no Hospital de Clínicas da Universidade Federal de Uberlândia, entre janeiro de 1974 e dezembro de 2002. FORMA DE ESTUDO: coorte historica. PACIENTES E MÉTODO: Duzentos e noventa e três pacientes foram submetidos à redução de fratura de mandíbula e retrospectivamente foram avaliados segundo fatores relacionados a: paciente, trauma, quadro clínico e tratamento cirúrgico. RESULTADOS: Houve uma clara tendência de aumento do número de fraturas de mandíbula ao longo dos anos. Houve um predomínio no sexo masculino (4:1, com pico de ocorrência entre 20 a 29 anos. As principais causas de fratura da mandíbula neste estudo foram acidentes de trânsito e violência, perfazendo juntas 72,4%. Cento e trinta e cinco pacientes apresentavam fratura única. Os sítios mais acometidos foram, em ordem decrescente, sínfise, côndilo, ângulo, corpo, ramo e processo coronóide. Foram realizadas redução incruenta (28, cruenta (213 e associação das duas (11 pacientes, sendo que 56,8% dos pacientes foram tratados nos primeiros 3 dias e 50,4% recebeu a alta hospitalar até o primeiro pós-operatório. Cerca de 10% dos pacientes apresentaram complicações, sendo osteomielite a mais freqüente. CONCLUSÃO: A incidência de fraturas de mandíbula foi marcadamente maior no sexo masculino, durante a terceira década de vida. A causa mais comum foi o acidente de trânsito e as regiões mais atingidas foram sínfise e côndilo. As fraturas isoladas de mandíbula ocorreram em mais de metade dos casos. A maioria dos pacientes foi tratada nos primeiros três dias e recebeu alta até o primeiro pós-operatório. A redução cruenta

Facts and fiction: the death of Saint Ignatius of Loyola.

Science.gov (United States)

Rosenman, L D

1996-01-01

The biography of Ignatius of Loyola, the sainted founder of the Society of Jesus (the Jesuits), ends with his death in curious circumstances. A careful search of the available sources written from the time of his death in 1556 to the present has reviewed the evidence. The evidence does not support the claims for a diagnosis that has been used for four centuries. So-called historical "facts" simply are the opinions of historians. Ignatius may have suffered from hyperparathyroidism that led to his death rather than from a cholangioportal venous fistula.

A reactivity accidents simulation of the Fort Saint Vrain HTGR

International Nuclear Information System (INIS)

Fainer, Gerson

1980-01-01

A reactivity accidents analysis of the Fort Saint Vrain HTGR was made. The following accidents were analysed 1) A rod pair withdrawal accident during normal operation, 2) A rod pair ejection accident, 3) A rod pair withdrawal accident during startup operations at source levels and 4) Multiple rod pair withdrawal accident. All the simulations were performed by using the BLOOST-6 nuclear code The steady state reactor operation results obtained with the code were consistent with the design reactor data. The numerical analysis showed that all accidents - except the first one - cause particle failure. (author)

Study of methyl bromide reactivity with human and mouse hemoglobin

African Journals Online (AJOL)

A study has been carried out on in-vitro reactivity of human and mouse hemoglobin spectrophotometrically at physiological pH, using different protein to reagent ratios. Hemoglobin side chains were modified with different concentrations of methyl bromide on agro-soil fumigant. To ascertain if the site of alkylation was the ...

Induction of nano pore in Agrobacterial hemoglobin

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Mojtaba Tousheh

2014-01-01

Full Text Available Introduction: A variety of oxygen-transport and -binding proteins exist in organisms including bacteria, protozoans, and fungi all have hemoglobin-like proteins. In addition to dealing with transport and sensing of oxygen, they may also deal with NO2, CO2, sulfide compounds, and even O2 scavenging in environments. Also they detoxified chlorinated materials like P450 enzymes and peroxidases and use as a detector of nitrate and hydrogen peroxide. Pore-forming bacterial globins are interested for filtration. Materials and methods: Although there are data for bacterial toxin as a filter, here we used Agrobacterial hem to induce nano pore in the heme structure using point mutation. Results: Investigations showed that three amino acids leucine 76, alanine 83 and histidine 80 are important for pore formation in Agrobacterium hemoglobin. A point mutation on leucine 76 to glycine, histidine 80 to asparagine and alanine 83 to lysine step by step led to create the nano pore 0.7- 0.8 nm in the globin. Discussion and conclusion: These mutations in bacterial hemoglobin increase the stability when mutation is with it’s at pH7. This mutation decreases the aliphatic index however increase the stability index.

On the Life and Deeds of San Precario, Patron Saint of Precarious Workers and Lives

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Marcello Tarì

2005-01-01

Full Text Available Noi siamo la generazione post-socialista, la generazione del dopo guerra fredda, della fine delle burocrazie verticali e del controllo sull'informazione. Siamo un movimento globale e neuropeo, che porta avanti la rivoluzione democratica scaturita dal Sessantotto mondiale e lotta contro la distopia neoliberista oggi al culmine. Siamo ecoattivisti e mediattivisti, siamo i libertari della Rete e i metroradicali dello spazio urbano, siamo le mutazioni transgender del femminismo globale, siamo gli hacker del terribile reale. Siamo gli agitatori del precariato e gli insorti del cognitariato. Siamo anarcosindacalisti e postsocialisti. Siamo tutti migranti alla ricerca di una vita migliore. E non ci iconosciamo in voi, stratificazioni tetre e tetragone di ceti politici sconfitti già nel XX secolo. Non ci riconosciamo nella sinistra italyana.Manifesto Bio/Pop del Precariato Metroradicale, 20042004 has marked the beginning of the spreading "cult" of San Precario, Patron Saint of precarious, casual, sessional, temporary, flexible and fractional workers. The Saint appears in public spaces in occasion of rallies, marches, interventions and demonstrations, and its popularity has lead to development of a precise and colorful iconography, hagiography and rituals. This popularity conversely is also the sign of the gravity of the issue of precarity in Italy and Europe .San Precario epitomises current Italian activist practices. These practices, although specifically Italian, intersect with similar realities in Europe and are based on mythopoetic narratives and actions and mediatic embodiments. This paper analysis a variety of texts produced around San Precario, from posters, saint cards, product cards, videos, "official" narratives, personal accounts, relating them to the political debate surrounding precarity.

Purification, characterization and sequence analyses of the extracellular giant hemoglobin from Oligobrachia mashikoi

OpenAIRE

Nakagawa, Taro; Onoda, Seiko; Kanemori, Masaaki; Sasayama, Yuichi; Fukumori, Yoshihiro

2005-01-01

We purified an extracellular hemoglobin with the molecular mass of ca. 440 kDa from the whole homogenates of Oligobrachia mashikoi (phylum Pogonophora) by a one-step gel-filtration. The preparation was pure to be crystallized. The P50 values of the hemoglobin and the fresh blood prepared from O. mashikoi were about 0.82 Torr and 0.9 Torr, respectively, which were much lower than the P50 value of human hemoglobin. However, the n values of the hemoglobin and the blood were about 1.2 and 1.1, re...

Blood hemoglobin level and treatment outcome of early breast cancer

International Nuclear Information System (INIS)

Henke, M.; Sindlinger, F.; Ikenberg, H.; Gerds, T.; Schumacher, M.

2004-01-01

Background and purpose: to determine whether the blood hemoglobin concentration correlates with the prognosis of patients with early breast cancer and, if so, whether this is restricted to treatment modality. Patients and methods: data were collected retrospectively from patients with early breast cancer (T1,2 NO-2 MO) who underwent either breast-conserving surgery followed by adjuvant radiotherapy (BCS-RT; n = 96) or a modified radical mastectomy (MRM; n = 194). The effect of preoperative blood hemoglobin level, nodal status, histological grading and hormone receptor status on disease-free survival was determined for both treatment modalities using a cox regression model and visualized by kaplan-meier plots. Results: the blood hemoglobin concentration significantly correlated with disease-free survival of patients receiving BCS-RT (relative risk [RR]: 0.67 per g/dl; p = 0.007). This was independent of other known risk factors for breast cancer patients, as determined by multivariate analysis. By contrast, the blood hemoglobin level had no prognostic significance when patients were treated with MRM. Conclusion: blood hemoglobin concentration seems to affect the prognosis of patients with early breast cancer when a treatment schedule that includes radiotherapy is applied. Reduced radiosensitivity due to diminished tumor oxygenation may be the underlying cause. Confirmative trials and studies intended to elucidate the underlying mechanism are warranted. (orig.)

NITRITE REDUCTASE ACTIVITY OF NON-SYMBIOTIC HEMOGLOBINS FROM ARABIDOPSIS THALIANA†

Science.gov (United States)

Tiso, Mauro; Tejero, Jesús; Kenney, Claire; Frizzell, Sheila; Gladwin, Mark T.

2013-01-01

Plant non-symbiotic hemoglobins possess hexa-coordinate heme geometry similar to the heme protein neuroglobin. We recently discovered that deoxygenated neuroglobin converts nitrite to nitric oxide (NO), an important signaling molecule involved in many processes in plants. We sought to determine whether Arabidopsis thaliana non-symbiotic hemoglobins class 1 and 2 (AHb1 and AHb2) might function as nitrite reductases. We found that the reaction of nitrite with deoxygenated AHb1 and AHb2 generates NO gas and iron-nitrosyl-hemoglobin species. The bimolecular rate constants for nitrite reduction to NO are 19.8 ± 3.2 and 4.9 ± 0.2 M−1s−1, at pH = 7.4 and 25°C, respectively. We determined the pH dependence of these bimolecular rate constants and found a linear correlation with the concentration of protons, indicating the requirement for one proton in the reaction. Release of free NO gas during reaction in anoxic and hypoxic (2% oxygen) conditions was confirmed by chemiluminescence detection. These results demonstrate that deoxygenated AHb1 and AHb2 reduce nitrite to form NO via a mechanism analogous to that observed for hemoglobin, myoglobin and neuroglobin. Our findings suggest that during severe hypoxia and in the anaerobic plant roots, especially in water submerged species, non-symbiotic hemoglobins provide a viable pathway for NO generation via nitrite reduction. PMID:22620259

La théorie de la diplomatie dans le Saint Empire Romain

OpenAIRE

Weber, Wolfgang E. J.

2016-01-01

Principes Dans ce que l’on nomme officiellement, depuis 1500 environ jusqu’à sa fin en 1806, le Saint Empire romain de la nation allemande (Heiliges Römisches Reich Deutscher Nation), le développement de la théorie de la diplomatie, autrement dit de conceptions relatives aux diplomates et à la diplomatie, est le fait d’élites soucieuses de mieux comprendre des problèmes posé...

Hemoglobin alpha 2 gene +861 G>A polymorphism in Turkish ...

African Journals Online (AJOL)

Thalassemia is an inherited blood disorder which is divided into two groups: alpha and beta. HBA1 and HBA2 are the two genes associated with alpha thalassemia. The aim of this study is to investigate abnormal hemoglobin variants of alpha globin gene in healthy abnormal hemoglobin carrying individuals with intact beta ...

Individualized Anemia Management Reduces Hemoglobin Variability in Hemodialysis Patients

OpenAIRE

Gaweda, Adam E.; Aronoff, George R.; Jacobs, Alfred A.; Rai, Shesh N.; Brier, Michael E.

2013-01-01

One-size-fits-all protocol-based approaches to anemia management with erythropoiesis-stimulating agents (ESAs) may result in undesired patterns of hemoglobin variability. In this single-center, double-blind, randomized controlled trial, we tested the hypothesis that individualized dosing of ESA improves hemoglobin variability over a standard population-based approach. We enrolled 62 hemodialysis patients and followed them over a 12-month period. Patients were randomly assigned to receive ESA ...

Hemoglobin redux: combining neutron and X-ray diffraction with mass spectrometry to analyse the quaternary state of oxidized hemoglobins

Energy Technology Data Exchange (ETDEWEB)

Mueser, Timothy C., E-mail: timothy.mueser@utoledo.edu; Griffith, Wendell P. [Department of Chemistry, University of Toledo, Toledo, OH 43606 (United States); Kovalevsky, Andrey Y. [Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Guo, Jingshu; Seaver, Sean [Department of Chemistry, University of Toledo, Toledo, OH 43606 (United States); Langan, Paul [Department of Chemistry, University of Toledo, Toledo, OH 43606 (United States); Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Hanson, B. Leif [Department of Chemistry, University of Toledo, Toledo, OH 43606 (United States)

2010-11-01

X-ray and neutron diffraction studies of cyanomethemoglobin are being used to evaluate the structural waters within the dimer–dimer interface involved in quaternary-state transitions. Improvements in neutron diffraction instrumentation are affording the opportunity to re-examine the structures of vertebrate hemoglobins and to interrogate proton and solvent position changes between the different quaternary states of the protein. For hemoglobins of unknown primary sequence, structural studies of cyanomethemoglobin (CNmetHb) are being used to help to resolve sequence ambiguity in the mass spectra. These studies have also provided additional structural evidence for the involvement of oxidized hemoglobin in the process of erythrocyte senescence. X-ray crystal studies of Tibetan snow leopard CNmetHb have shown that this protein crystallizes in the B state, a structure with a more open dyad, which possibly has relevance to RBC band 3 protein binding and erythrocyte senescence. R-state equine CNmetHb crystal studies elaborate the solvent differences in the switch and hinge region compared with a human deoxyhemoglobin T-state neutron structure. Lastly, comparison of histidine protonation between the T and R state should enumerate the Bohr-effect protons.

Consumer preferences and willingness to pay for compact fluorescent lighting: Policy implications for energy efficiency promotion in Saint Lucia

International Nuclear Information System (INIS)

Reynolds, Travis; Kolodinsky, Jane; Murray, Byron

2012-01-01

This article examines consumer willingness to pay for energy-saving compact fluorescent light bulbs using the results of a stated preferences study conducted in the Caribbean island nation of Saint Lucia. Geographic location, low income status, and age are found to affect willingness-to-pay for compact fluorescent lighting, while higher income status and other demographic variables appear to have minimal or no significant impacts. Energy efficiency knowledge is associated with increased willingness-to-pay for energy-efficient bulbs and with increased use of compact fluorescent lighting. Contrary to theoretical expectations, past purchase of compact fluorescent bulbs is found to have no impact on self-reported willingness to pay. We hypothesize that this null result is due to the recent emergence of low-cost, low-quality compact fluorescent bulbs in the Saint Lucian lighting market, which may be negatively influencing consumers' preferences and expectations regarding energy-efficient lighting. Findings support the argument that government-sponsored education and subsidy programs will likely result in increased use of energy-saving technologies in Saint Lucia. But such behavioral changes may not be sustained in the long run unless low quality bulbs – the “lemons” of the compact fluorescent lighting market – can be clearly identified by consumers. - Highlights: ▶ We model how knowledge, attitudes, and past purchase affect CFL adoption. ▶ Saint Lucian consumers have some knowledge of and favorable attitudes toward CFLs. ▶ Energy efficiency knowledge increases stated willingness-to-pay (WTP) for CFLs. ▶ Past purchase does not increase WTP; low-quality ‘lemons’ may influence consumers. ▶ Policy can lower consumer risks in lighting markets where low quality bulbs exist.

Ciguatera fish poisoning in an international ship crew in Saint John Canada: 2015.

Science.gov (United States)

Muecke, C; Hamper, L; Skinner, A L; Osborne, C

2015-11-05

An international ship crew presented for medical care in Saint John, New Brunswick, following rapid onset of gastrointestinal and in some cases neurological and cardiac symptoms after a common fish meal. Ciguatera poisoning was identified as the cause of illness. This report describes the public health investigation and management of this incident, including collaboration between the implicated provincial and federal authorities.

Prayer to the Saints or the Virgin and Health among Older Mexican Americans

Science.gov (United States)

Krause, Neal; Bastida, Elena

2011-01-01

The purpose of this study was to evaluate a conceptual model that assesses whether praying to the saints or the Virgin is associated with the health of older Mexican Americans. A survey was conducted of 1,005 older Mexican Americans (Mean age = 73.9 years; SD = 6.6 years). Data from 795 of the Catholic respondents are presented in this study. The…

A second level of the Saint Petersburg skyline

Science.gov (United States)

Krasnopolsky, Andrey; Bolotin, Sergey

2018-03-01

The article considers the history of the residential development in Saint Petersburg and states corresponding landmark dates. In recent years, changes in the altitude range of the residential development are noted, the influence of this factor on the formation of the city's silhouette is assessed. Reasons for such changes are identified. Attractiveness of high-rise residential complexes for living is assessed. Conclusions are made of tendencies in further housing construction development in terms of its altitude range. It is noted that it is possible to locate multi-storied buildings in the periphery of the city, taking into account specific visual characteristics of the construction site and silhouette of erected buildings; as for central districts, strict regulations regarding the altitude range are needed.

Convenio de contratación para el proyecto de la iglesia Saint-Pierre de Firminy-Vert

Directory of Open Access Journals (Sweden)

Merwan Chaverra Suárez

2010-12-01

Full Text Available Los pormenores del proceso de contratación para el encargo de la iglesia Saint-Pierre de Firminy-Vert, entre 1960 y 1963, corresponde al desglose de las conversaciones e intercambios de correspondencia entre el arquitecto y sus clientes, que dieron lugar a un número de versiones y variantes del proyecto, así como a dificultades administrativas y económicas, que se prolongaron en el tiempo, hasta la firma del contrato. El proyecto de la iglesia Saint-Pierre de Firminy-Vert cuenta con un dossier completo, guardado y archivado en la Fundación Le Corbusier, que garantiza poder efectuar un análisis total del proyecto, enfocándolo en una metodología que posibilite el entendimiento y la reconstrucción de los hechos a partir de las fuentes primarias.

TRUNCATED OR 2/2 HEMOGLOBINS : A NEW CLASS OF GLOBINS WITH NOVEL STRUCTURE AND FUNCTION

Directory of Open Access Journals (Sweden)

Amit Kumar

2013-06-01

Full Text Available Bright red hemoglobins, the most well-known paradigm in protein science, seem to be ubiquitous in nature. With advances in modern tools and techniques, discovery of new globins at a rapid pace has expanded this family. With every discovery, new insights emerged regarding their novel structure, function and several other characteristics previously not observed for hemoglobins. Even the classical function unanimously assigned to hemoglobins – oxygen transport and storage – needed re-evaluation. The ability of this class of proteins to show responses against various gaseous ligands, even the poisonous ones, indicate that it is obviously as ancient as life. As organisms evolved, hemoglobins also evolved, and accumulated a great degree of diversity in all aspects. The classical globin fold is very unique with 3-on-3 alpha helical bundle as observed in the traditional oxygen-transport hemoglobins like myoglobin, human blood hemoglobin and leghemoglobins in plants. However, a class of the newly discovered hemoglobins, which dominate the superfamily and appears ancient in origin mostly have 2-on-2 fold, commonly termed as “truncated” hemoglobins. These hemoglobins are phylogenetically distinct from their classical counterparts and are often shorter in their polypeptide length by 20-40 residues mainly due to a lack of short A helix, D helix and F helix. However, hemoglobins with 2-on-2 fold were also later found to have polypeptide chain lengths similar in size to classical globins. Disordered pre-F helix region, conserved glycine motifs and other key residues and apolar tunnels through their protein matrix for migration of ligands are some unique characteristics features of these truncated hemoglobins. Some of these are also hexacoordinated at heme iron where an amino acid from within the protein coordinates heme iron in absence of a ligand. These hemoglobins are well known for their high affinity towards ligand and have a diverse mechanism of

High-rise construction in the Saint Petersburg agglomeration in 1703-1950s

Science.gov (United States)

Sementsov, Sergey; Akulova, Nadezhda; Kurakina, Severina

2018-03-01

Regularities of high-rise construction (implemented projects and developments) in Saint Petersburg and the Saint Petersburg agglomeration since the foundation of the city in 1703 till the 1950s are considered. Based on these regularities, a single spatially developed system of vertical dominants is formed. High-rise construction in the city and its suburbs started in the 1710s and continues up to the present time. In the considered decades (1703-1950s), high-rise construction mostly performed urban-planning functions (with vertical and symbolic dominants), relying on patterns of the visual perception of man-made landscapes under development. Since the 1710s, the construction of vertical dominants (mainly temples, spires of towers, lighthouses, etc.) of five ranks (depending on the altitude range and in relation to the background development) was conducted in territories of the entire agglomeration. These dominants were arranged in landscapes of the city and suburbs with almost mathematically precise accuracy and according to special regulations. Such dominants obtained particular descriptive and silhouette characteristics in accordance with the conditions of spatial perception. In some periods of city development, attempts were made to create monuments (symbolic dominants) of specific height and include those in the spatial system of high-rise dominants as significant elements of the city silhouette.

PREFACE: 1st International School and Conference "Saint Petersburg OPEN 2014" on Optoelectronics, Photonics, Engineering and Nanostructures

Science.gov (United States)

2014-09-01

Dear Colleagues, 1st International School and Conference "Saint Petersburg OPEN 2014" on Optoelectronics, Photonics, Engineering and Nanostructures was held on March 25 - 27, 2014 at St. Petersburg Academic University - Nanotechnology Research and Education Centre of the Russian Academy of Sciences. The School and Conference included a series of invited talks given by leading professors with the aim to introduce young scientists with actual problems and major advances in physics and technology. The keynote speakers were: Mikhail Glazov (Ioffe Physico-Technical Institute RAS, Russia) Vladimir Dubrovskii (Saint Petersburg Academic University RAS, Russia) Alexey Kavokin (University of Southampton, United Kingdom and St. Petersburg State University, Russia) Vladimir Korenev (Ioffe Physico-Technical Institute RAS, Russia) Sergey Kukushkin (Institute of Problems of Mechanical Engineering RAS, Russia) Nikita Pikhtin (Ioffe Physico-Technical Institute RAS, Russia and "Elfolum" Ltd., Russia) Dmitry Firsov (Saint Petersburg State Polytechnical University, Russia) During the poster session all undergraduate and graduate students attending the conference presented their works. Sufficiently large number of participants with more than 160 student attendees from all over the world allowed the Conference to provide a fertile ground for the fruitful discussions between the young scientists as well as to become a perfect platform for the valuable discussions between student authors and highly experienced scientists. The best student papers, which were selected by the Program Committee and by the invited speakers basing on the theses and their poster presentation, were awarded with diplomas of the conference - see the photos. This year's School and Conference is supported by SPIE (The International Society for Optics and Photonics), OSA (The Optical Society), St. Petersburg State Polytechnical University and by Skolkovo Foundation. It is a continuation of the annual schools and

Nondestructive evaluation of the oxidation and strength of the Fort Saint Vrain HTGR support block

International Nuclear Information System (INIS)

Tingey, G.L.; Posakony, G.J.; Morgan, W.C.; Prince, J.M.; Hill, R.W.; Lessor, D.L.

1982-04-01

Non-destructive detection of changes in the strength of graphite support structures in a HTGR appears to be feasible using sonic velocity measurements where access for through transmission is possible. Therefore, future HTGR designs should consider providing such access. Where access is not available, strength changes can be correlated with oxidation profiles in the support member. These oxidation profiles can be determined non-destructively by a combination of eddy current measurements to detect near surface oxidation and sonic backscattering measurements designed to determine oxidation in depth. The Fort Saint Vrain reactor provides an operating reactor to test the applicability of the eddy current and sonic backscattering techniques for determination of oxidation in a support block. Furthermore, such tests in Fort Saint Vrain will supply base line data which will be useful in assuring an adequate strength of the support structure for the lifetime of the reactor. Equipment is, therefore, being developed for tests to be conducted during the next major refueling of the reactor

Thalassemia and Hemoglobin E in Southern Thai Blood Donors

OpenAIRE

Nuinoon, Manit; Kruachan, Kwanta; Sengking, Warachaya; Horpet, Dararat; Sungyuan, Ubol

2014-01-01

Thalassemia and hemoglobin E (Hb E) are common in Thailand. Individuals with thalassemia trait usually have a normal hemoglobin concentration or mild anemia. Therefore, thalassemic individuals who have minimum acceptable Hb level may be accepted as blood donors. This study was aimed at determining the frequency of α-thalassemia 1 trait, β-thalassemia trait, and Hb E-related syndromes in Southern Thai blood donors. One hundred and sixteen voluntary blood donors, Southern Thailand origin, were ...

Structural characterization of hemoglobins from Monilifera and Frenulata tubeworms (Siboglinids): first discovery of giant hexagonal-bilayer hemoglobin in the former "Pogonophora" group.

Science.gov (United States)

Meunier, Cédric; Andersen, Ann C; Bruneaux, Matthieu; Le Guen, Dominique; Terrier, Peran; Leize-Wagner, Emmanuelle; Zal, Franck

2010-01-01

Siboglinids are symbiotic polychete annelids having hemoglobins as essential oxygen- and sulfide-carriers for their endosymbiotic bacteria. We analyzed the structure of the hemoglobins from two species of siboglinids: the monilifera Sclerolinum contortum and the frenulata Oligobrachia webbi (i.e. haakonmosbiensis) from Norwegian cold seeps. Measured by Multi-Angle Laser Light Scattering (MALLS), Sclerolinum shows a 3190+/-50 kDa hexagonal bilayer hemoglobin (HBL-Hb) and a 461+/-46 kDa ring-Hb, just as vestimentifera, whereas Oligobrachia has a 409+/-3.7 kDa ring-Hb only. Electrospray Ionization-Mass Spectrometry (ESI-MS) showed Sclerolinum HBL-Hb composed of seven monomeric globins (15-16 kDa), three disulfide-bonded globin heterodimers and three linkers. The heterodimers always contain globin-b (15814.4+/-1.5 Da). Sclerolinum ring-Hb is composed of globins and dimers with identical masses as its HBL-Hb, but lacks linkers. Oligobrachia ring-Hb has three globin monomers (14-15 kDa) only, with no disulfide-bonded dimers. Comparison of Sclerolinum hemoglobins between Storegga and Haakon Mosby Mud Volcano, using the normalized height of deconvoluted ESI-MS peaks, shows differences in globin monomers abundances that could reflect genetic differences or differential gene expression between distinct seep populations. The discovery of HBL-Hb in Sclerolinum is a new element supporting the hypothesis of monilifera being phylogenetically more closely related to vestimentifera, than to frenulata.

The role of nitric oxide and hemoglobin in plant development and morphogenesis

DEFF Research Database (Denmark)

Hebelstrup, Kim; Shah, Jay K; Igamberdiev, Abir U

2013-01-01

effects on control of plant growth and development, such as shoot and root architecture. All plants are able to express non-symbiotic hemoglobins at low concentration. Their function is generally not related to oxygen transport or storage; instead they effectively oxidize NO to NO3– and thereby control...... the local cellular NO concentration. In this review, we analyze available data on the role of NO and plant hemoglobins in morphogenetic processes in plants. The comparison of the data suggests that hemoglobin gene expression in plants modulates development and morphogenesis of organs, such as roots...... and shoots, through the localized control of NO, and that hemoglobin gene expression should always be considered a modulating factor in processes controlled directly or indirectly by NO in plants....

Identifying Key Stakeholder Groups for Implementing a Place Branding Policy in Saint Petersburg

Directory of Open Access Journals (Sweden)

Kulibanova V. V.

2017-10-01

Full Text Available Regional brands have become a valuable intangible asset and a crucial competitive resource for forging partnerships. An effective place branding policy is impossible without a precise understanding of the interests of stakeholder groups. It is essential to realize that each region is unique in its own way. Territories differ in the structure of stakeholders, their influence on regional development, and the range of leverages over regional decision-makers. This study aims to give a more precise definition of key groups of stakeholders in Saint Petersburg place branding, and to identify them. The authors employ the method of theoretical and empirical typology of a territory’s stakeholders within a theoretical framework proposed by E. Freeman, P. Kotler, S. Zenker, and E. Brown. The article defines the concept of key regional stakeholders and identifies them. The proposed target audience (stakeholder group model for a place branding policy is tested on the case of Saint Petersburg. The authors show that each target audience of place marketing requires an individual policy. This is explained by the fact that each group enjoys its unique features that should be taken into account when creating and transmitting messages.

Imidazolidinone adducts of peptides and hemoglobin

International Nuclear Information System (INIS)

San George, R.C.; Hoberman, H.D.

1986-01-01

Acetaldehyde reacts selectively with the terminal amino groups of the α and β chains of hemoglobin to form stable adducts, the structures of which, based on 13 C NMR studies, are proposed to be diastereomeric 2-methyl imidazolidin-4-ones. In this scheme, acetaldelhyde forms a reversible Schiff base with the α-amino groups of the polypeptide chains which cyclize with the amide nitrogen of the first peptide bond to form the stable imidazolidinone adducts. In support of this mechanism, the authors found that in following the reaction of the peptide val-gly-gly with [1,2- 13 C] acetaldehyde, 13 C NMR resonances attributed to a Schiff base (δ = 170 ppm) were observed which slowly disappeared prior to appearance of resonances from a pair of stable adducts (δ = 70 and 71 ppm) believed to be the diastereomeric imidazolidinones. Schiff base formation appeared to limit the overall rate. Tetraglycine reacted in a similar manner but with a resonance from a single stable adduct observed representing the enantiomeric imidazolidinone adducts of this peptide. Peptides with proline in position 2 should be incapable of forming imidazolidinones, and the authors found that ala-pro-gly did in fact fail to form a stable adduct with acetaldehyde. The 2-methyl imidazolidin-4-one adducts of hemoglobin may be useful in determining the contribution of the amino terminal groups to the structure and functional properties of hemoglobins

Sainte-Bernardette du Banlay, Nevers

Directory of Open Access Journals (Sweden)

Christophe Joly

2009-11-01

Full Text Available Construite par Claude Parent et Paul Virilio en 1964-1965, l’église Sainte-Bernadette du Banlay témoigne de leurs travaux sur la fonction oblique. L’édifice fut édifié à l’issue d’un concours d’architecture lancé en 1963 par Mgr Vial et l’Abbé Bourgoin, curé de la paroisse. L’église est inspirée de l’architecture des bunkers. Deux coques de béton s’imbriquent l’une dans l’autre en se décalant par rapport à l’axe longitudinal. L’apparence générale est un édifice monolithique, qui évoque un rocher. Á l’intérieur, deux grands plans obliques paraissent exhumés pour constituer la nef et le chœur. En relation étroite avec la grotte des apparitions de Lourdes, l’église est construite comme une crypte suspendue dont la coupe adopte la forme d’un cœur. Par le choix de matériaux bruts et d’un éclairage zénithal, les architectes confèrent à l’ensemble une étonnante intimité. C’est par l’opposition entre le volume extérieur austère et fermé et l’espace intérieur accueillant et protecteur que les concepteurs expriment la nouvelle fonction d’une église conçue dans une période d’incertitude et de tensions.The Sainte-Bernadette du Banlay church at Nevers was built from 1964 to 1965 to the designs of the architects Claude Parent and Paul Virilio, working on their theme of the oblique function. It was the fruit of an architectural competition organised in 1963 by Monseigneur Vial and the Abbé Bourgoin, the parish priest. The church’s architecture is inspired by that of defensive bunkers. Two reinforced concrete shells are joined the one into the other but out of true on a longitudinal axis. The overall appearance of the church is that of a monolithic block, something like a rock. Inside, two large oblique planes seem to emerge to create the nave and the chancel. Closely related to the Lourdes grotto of the Virgin’s appearances, the church is designed as a suspended crypt

Distribution of some organochloride pesticides in the All Saints Bay, Bahia, Brazil

International Nuclear Information System (INIS)

Beretta, M.; Costa, M.A.; Tavares, T.M.

1999-01-01

First restrictions to organochlorine insecticides (OC) in Brazil date of 1976 and by 1985 they were completely prohibited. However use of existing stocks and smuggled parcels as well as official use in malaria campaigns still takes place. During the SH summer of 1995 a cross-sectional survey was conducted for organochloride pesticides in 12 intertidal sites, both in sediments and in some selected edible species of the All Saints Bay, east of Brazil

Crystal structure of hemoglobin from the maned wolf (Chrysocyon brachyurus) using synchrotron radiation.

Science.gov (United States)

Fadel, Valmir; Canduri, Fernanda; Olivieri, Johnny R; Smarra, André L S; Colombo, Marcio F; Bonilla-Rodriguez, Gustavo O; de Azevedo, Walter F

2003-12-01

Crystal structure of hemoglobin isolated from the Brazilian maned wolf (Chrysocyon brachyurus) was determined using standard molecular replacement technique and refined using maximum-likelihood and simulated annealing protocols to 1.87A resolution. Structural and functional comparisons between hemoglobins from the Chrysocyon brachyurus and Homo sapiens are discussed, in order to provide further insights in the comparative biochemistry of vertebrate hemoglobins.

Correlation of maternal factors and hemoglobin concentration during pregnancy Shiraz 2006

Directory of Open Access Journals (Sweden)

Marzieh Akbarzadeh

2009-12-01

Full Text Available Background: Anemia in pregnancy is a serious condition, contributing to maternal mortality, morbidity and fetal morbidity and its prevalence varies between 35-100% in developing countries. This investigation is conducted to survey the correlation of maternal factors and the changes in hemoglobin in pregnant women. Method: In this study, 108 healthy pregnant women with gestational age of 10 to 14 weeks, chosen by cluster random sampling were included. The women were followed in three visits: at the end of the first, second and third trimester. In addition, correlation of Hb concentration with maternal factors including BMI, age parity, hyperemesis, gestational age, pregnancy interval and weight gain was investigated. Results: There was no significant correlation between BMI, parity, pregnancy interval, severe nausea and vomiting and also maternal age with hemoglobin level during pregnancy. Moreover, Multiple regression models showed that adequate maternal weight gain (P<0.009 and high hemoglobin (p<0.0001 in the first trimester were positive predictors and late iron supplementation was negative predictor of hemoglobin in pregnancy (P<0.006. Conclusion: Our data demonstrated that adequate maternal weight gain, high hemoglobin in the first trimester and also late iron supplementation could be as predictors in clinical settings in this query.

Characterization of trypsin-derived peptides acrylamide-adducted hemoglobin

International Nuclear Information System (INIS)

Springer, D.L.; Goheen, S.C.; Edmonds, C.G.; McCulloch, M.; Sylvester, D.M.; Sander, C.; Bull, R.J.

1991-01-01

Even though there are a number of sources for human exposure to acrylamide, reliable biomarkers of exposure are not available. In an effort to develop such a biomarker, the authors are characterizing peptides derived from trypsin digests of acrylamide-adducted hemoglobin. For this, radiolabeled acrylamide was incubated with this, radiolabeled acrylamide was incubated with purified human hemoglobin (Ao) and decomposition products removed by dialysis. When the adducted hemoglobin was separated by reverse-phase HPLC, radioactivity eluted with the α and β subunits, suggesting covalent binding. Digestion of individual subunits with trypsin followed by reverse phase HPLC, indicated that most of the radioactivity associated with the α subunit co-eluted with a single peptide. Similar results were observed for the β subunit except that significant amounts of radioactivity eluted with the solvent front, suggesting that radioactivity was released by trypsin digestion. Currently, these preparation are under further characterization by electrospray ionization mass spectrometry. This approach will aid in the identification of the adducted will aid in the identification of the adducted peptide and subsequent preparation of an acrylamide-specific antibody

Baking the Bread and Roasting the Meat : Dorlandus's Saint Lawrence as a model for Carthusians

NARCIS (Netherlands)

van Dijk, Mathilde

2018-01-01

The two sermons on Saint Lawrence by the profilic Carthusian author Petrus Dorlandus survive in a manuscript from the charterhouse in Louvain, which was used for reading at table. Dorlandus’s development of the well-known tale about this martyr is geared to this practice. Inviting his fellow

Hemoglobin levels and blood transfusion in patients with sepsis in Internal Medicine Departments.

Science.gov (United States)

Muady, Gassan Fuad; Bitterman, Haim; Laor, Arie; Vardi, Moshe; Urin, Vitally; Ghanem-Zoubi, Nesrin

2016-10-13

Acute reduction in hemoglobin levels is frequently seen during sepsis. Previous studies have focused on the management of anemia in patients with septic shock admitted to intensive care units (ICU's), including aggressive blood transfusion aiming to enhance tissue oxygenation. To study the changes in hemoglobin concentrations during the first week of sepsis in the setting of Internal Medicine (IM) units, and their correlation to survival. Observational prospective study. We recorded hemoglobin values upon admission and throughout the first week of hospital stay in a consecutive cohort of septic patients admitted to IM units at a community hospital, the patients were enrolled into a prospective registry. Data on blood transfusions was also collected, we examined the correlation between hemoglobin concentrations during the first week of sepsis and survival, the effect of blood transfusion was also assessed. Eight hundred and fifteen patients (815) with sepsis were enrolled between February 2008 to January 2009. More than 20 % of them had hemoglobin levels less than 10g/dL on admission, a rate that was doubled during the first week of sepsis. Overall, 68 (8.3 %) received blood transfusions, 14 of them (20.6 %) due to bleeding. Typically, blood transfusion was given to older patients with a higher rate of malignancy and lower hemoglobin levels. While hemoglobin concentration on admission had strong correlation with in-hospital mortality (O.R-0.83 [95 % C.I. 0.74-0.92], blood transfusion was not found to be an independent predicting factor for mortality. Anemia is very common in sepsis. While hemoglobin level on admission exhibit independent correlation with survival, blood transfusion do not.

The relationship between Type D personality, affective symptoms and hemoglobin levels in chronic heart failure.

Directory of Open Access Journals (Sweden)

Nina Kupper

Full Text Available BACKGROUND: Anemia is associated with poor prognosis in heart failure (HF patients. Contributors to the risk of anemia in HF include hemodilution, renal dysfunction and inflammation. Hemoglobin levels may also be negatively affected by alterations in stress regulatory systems. Therefore, psychological distress characterized by such alterations may adversely affect hemoglobin in HF. The association between hemoglobin and Type D personality and affective symptomatology in the context of HF is poorly understood. AIM: To examine the relationship between Type D personality and affective symptomatology with hemoglobin levels at inclusion and 12-month follow-up, controlling for relevant clinical factors. METHODS: Plasma levels of hemoglobin and creatinine were assessed in 264 HF patients at inclusion and at 12-month follow-up. Type D personality and affective symptomatology were assessed at inclusion. RESULTS: At inclusion, hemoglobin levels were similar for Type D and non-Type D HF patients (p = .23, and were moderately associated with affective symptomatology (r = -.14, p = .02. Multivariable regression showed that Type D personality (β = -.15; p = .02, was independently associated with future hemoglobin levels, while controlling for renal dysfunction, gender, NYHA class, time since diagnosis, BMI, the use of angiotensin-related medication, and levels of affective symptomatology. Change in renal function was associated with Type D personality (β = .20 and hemoglobin at 12 months (β = -.25. Sobel mediation analysis showed significant partial mediation of the Type D - hemoglobin association by renal function deterioration (p = .01. Anemia prevalence increased over time, especially in Type D patients. Female gender, poorer baseline renal function, deterioration of renal function and a longer HF history predicted the observed increase in anemia prevalence over time, while higher baseline hemoglobin was protective

Capillary versus Venous Hemoglobin Determination in the Assessment of Healthy Blood Donors

Science.gov (United States)

Patel, Abhilasha J.; Wesley, Robert; Leitman, Susan F.; Bryant, Barbara J.

2013-01-01

Background and Objectives To determine the accuracy of fingerstick hemoglobin assessment in blood donors, the performance of a portable hemoglobinometer (HemoCue Hb 201+) was prospectively compared with that of an automated hematology analyzer (Cell-Dyn 4000). Hemoglobin values obtained by the latter were used as the “true” result. Material and Methods Capillary fingerstick samples were assayed by HemoCue in 150 donors. Fingerstick samples from two sites, one on each hand, were obtained from a subset of 50 subjects. Concurrent venous samples were tested using both HemoCue and Cell-Dyn devices. Results Capillary hemoglobin values (HemoCue) were significantly greater than venous hemoglobin values (HemoCue), which in turn were significantly greater than venous hemoglobin values by Cell-Dyn (mean ± SD: 14.05 ± 1.51, 13.89 ± 1.31, 13.62 ± 1.23, respectively; phemoglobin screening criteria (≥12.5 g/dL) by capillary HemoCue, but were deferred by Cell-Dyn values (false-pass). Five donors (3%) were deferred by capillary sampling, but passed by Cell-Dyn (false-fail). Substantial variability in repeated fingerstick HemoCue results was seen (mean hemoglobin 13.72 vs. 13.70 g/dL, absolute mean difference between paired samples 0.76 g/dL). Hand dominance was not a factor. Conclusions Capillary samples assessed via a portable device yielded higher hemoglobin values than venous samples assessed on an automated analyzer. False-pass and false-fail rates were low and acceptable in the donor screening setting, with “true” values not differing by a clinically significant degree from threshold values used to assess acceptability for blood donation. PMID:23294266

Prognostic Factors Affecting Locally Recurrent Rectal Cancer and Clinical Significance of Hemoglobin

International Nuclear Information System (INIS)

Rades, Dirk; Kuhn, Hildegard; Schultze, Juergen; Homann, Nils; Brandenburg, Bernd; Schulte, Rainer; Krull, Andreas; Schild, Steven E.; Dunst, Juergen

2008-01-01

Purpose: To investigate potential prognostic factors, including hemoglobin levels before and during radiotherapy, for associations with survival and local control in patients with unirradiated locally recurrent rectal cancer. Patients and Methods: Ten potential prognostic factors were investigated in 94 patients receiving radiotherapy for recurrent rectal cancer: age (≤68 vs. ≥69 years), gender, Eastern Cooperative Oncology Group performance status (0-1 vs. 2-3), American Joint Committee on Cancer (AJCC) stage (≤II vs. III vs. IV), grading (G1-2 vs. G3), surgery, administration of chemotherapy, radiation dose (equivalent dose in 2-Gy fractions: ≤50 vs. >50 Gy), and hemoglobin levels before (<12 vs. ≥12 g/dL) and during (majority of levels: <12 vs. ≥12 g/dL) radiotherapy. Multivariate analyses were performed, including hemoglobin levels, either before or during radiotherapy (not both) because these are confounding variables. Results: Improved survival was associated with better performance status (p < 0.001), lower AJCC stage (p = 0.023), surgery (p = 0.011), chemotherapy (p = 0.003), and hemoglobin levels ≥12 g/dL both before (p = 0.031) and during (p < 0.001) radiotherapy. On multivariate analyses, performance status, AJCC stage, and hemoglobin levels during radiotherapy maintained significance. Improved local control was associated with better performance status (p = 0.040), lower AJCC stage (p = 0.010), lower grading (p = 0.012), surgery (p < 0.001), chemotherapy (p < 0.001), and hemoglobin levels ≥12 g/dL before (p < 0.001) and during (p < 0.001) radiotherapy. On multivariate analyses, chemotherapy, grading, and hemoglobin levels before and during radiotherapy remained significant. Subgroup analyses of the patients having surgery demonstrated the extent of resection to be significantly associated with local control (p = 0.011) but not with survival (p = 0.45). Conclusion: Predictors for outcome in patients who received radiotherapy for locally

Hemoglobin is essential for normal growth of Arabidopsis organs

DEFF Research Database (Denmark)

Hebelstrup, Kim Henrik; Hunt, Peter; Dennis, Elizabeth

2006-01-01

In Arabidopsis thaliana, the class I hemoglobin AHb1 is transiently expressed in the hydathodes of leaves and in floral buds from young inflorescences. Nitric oxide (NO) accumulates to high levels in these organs when AHb1 is silenced, indicating an important role in metabolizing NO. AHb1-silenced...... lines are viable but show a mutant phenotype affecting the regions where AHb1 is expressed. Arabidopsis lines with an insertional knockout or overexpression of AHb2, a class II 3-on-3 hemoglobin, were generated. Seedlings overexpressing AHb2 show enhanced survival of hypoxic stress. The AHb2 knockout...... lines develop normally. However, when AHb2 knockout is combined with AHb1 silencing, seedlings die at an early vegetative stage suggesting that the two 3-on-3 hemoglobins, AHb1 and AHb2, together play an essential role for normal development of Arabidopsis seedlings. In conclusion, these results...

Hemoglobin concentration determination based on near infrared spatially resolved transmission spectra

Science.gov (United States)

Zhang, Linna; Li, Gang; Lin, Ling

2016-10-01

Spatially resolved diffuse reflectance spectroscopy method has been proved to be more effective than single point spectroscopy method in the experiment to predict the concentration of the Intralipid diluted solutions. However, Intralipid diluted solution is simple, cannot be the representative of turbid liquids. Blood is a natural and meaningful turbid liquid, more complicate. Hemoglobin is the major constituent of the whole blood. And hemoglobin concentration is commonly used in clinical medicine to diagnose many diseases. In this paper, near infrared spatially resolved transmission spectra (NIRSRTS) and Partial Least Square Regression (PLSR) were used to predict the hemoglobin concentration of human blood. The results showed the prediction ability for hemoglobin concentration of the proposed method is better than single point transmission spectroscopy method. This paper demonstrated the feasibility of the spatially resolved diffuse reflectance spectroscopy method for practical liquid composition analysis. This research provided a new thinking of practical turbid liquid composition analysis.

Special investigations of hemoglobin in the dynamics of acute radiation sickness

International Nuclear Information System (INIS)

Zdravko, B.J.; Panasyuk, E.N.

1986-01-01

The effect of penetrating radiation into the UV, visible and IR spectra of hemoglobin obtained from guinea-pigs being irradiated by the 300 and 600 cGy doses is studied. The change of the absorption intensity in the range of 275 nm of aqueous hemoglobin solutions depending on the stage and duration of the radiation pathology is revealed. The displacement of amide absorption bands into a shorter area of hemoglobin fluctuations frequencies of irradiated animals in the period from the 1 to 19-th day after the irradiation by the 300 cGy dose and during the whole period of the acute radiation pathology after the irradiation by the 600 cGy dose is established by the use of the IR-spectroscopy method. For the relative quantitative estimation of the denaturized hemoglobins by radiation, radiotoxins and by other physical and chemical factors, one suggests to use the formulas of the hem optical density relation coefficient to the globin optical density

Hemoglobin Kinetics and Long-term Prognosis in Heart Failure.

Science.gov (United States)

Díez-López, Carles; Lupón, Josep; de Antonio, Marta; Zamora, Elisabet; Domingo, Mar; Santesmases, Javier; Troya, Maria-Isabel; Boldó, Maria; Bayes-Genis, Antoni

2016-09-01

The influence of hemoglobin kinetics on outcomes in heart failure has been incompletely established. Hemoglobin was determined at the first visit and at 6 months. Anemia was defined according to World Health Organization criteria (hemoglobin < 13g/dL for men and hemoglobin < 12g/dL for women). Patients were classified relative to their hemoglobin values as nonanemic (both measurements normal), transiently anemic (anemic at the first visit but not at 6 months), newly anemic (nonanemic initially but anemic at 6 months), or permanently anemic (anemic in both measurements). A total of 1173 consecutive patients (71.9% men, mean age 66.8±12.2 years) were included in the study. In all, 476 patients (40.6%) were considered nonanemic, 170 (14.5%) had transient anemia, 147 (12.5%) developed new-onset anemia, and 380 (32.4%) were persistently anemic. During a follow-up of 3.7±2.8 years after the 6-month visit, 494 patients died. On comprehensive multivariable analyses, anemia (P < .001) and the type of anemia (P < .001) remained as independent predictors of all-cause mortality. Compared with patients without anemia, patients with persistent anemia (hazard ratio [HR] = 1.62; 95% confidence interval [95%CI], 1.30-2.03; P < .001) and new-onset anemia (HR = 1.39; 95%CI, 1.04-1.87, P = .03) had higher mortality, and even transient anemia showed a similar trend, although without reaching statistical significance (HR = 1.31; 95%CI, 0.97-1.77, P = .075). Anemia, especially persistent and of new-onset, and to a lesser degree, transient anemia, is deleterious in heart failure. Copyright © 2016 Sociedad Española de Cardiología. Published by Elsevier España, S.L.U. All rights reserved.

Training needs assessment of health care professionals in a developing country: the example of Saint Lucia.

Science.gov (United States)

Gaspard, Janice; Yang, Che-Ming

2016-04-16

Continuing education (CE) is crucial for quality improvement in health care. The needs assessment of CE helps ensure effectiveness. However, such an assessment necessitates certain techniques that are unfamiliar to health care communities in developing countries. This study identifies the needs of providing CE to health care personnel in Saint Lucia. This study was designed as a questionnaire survey to investigate the demographics, training needs, and preferred approaches to improve performance of the target population. The study population included the health care professionals of major public health care facilities in Saint Lucia. We used the World Health Organization-adopted Hennessy Hicks Training Needs Analysis Questionnaire, a self-reported close-ended structured questionnaire with a core set of 30 items. These items refer to tasks that are central to the role of health care professionals and are categorized into six superordinate categories: research/audit, communication/teamwork, clinical skills, administrative, managerial/supervisory, and continuing professional education. In total, 208 questionnaires were distributed; the response rate was 66.8%, and most respondents were nurses. The need for continuing professional education was rated the highest priority, followed by research/audit activities. The evidence suggests that most respondents required training in communication skills, management, clinical skills, and research methods. Providing training according to the needs is vital, particularly in developing countries. The present research methodology and findings offer perspectives on how to conduct needs assessment and offer reference points for developing countries whose background and health care environment are similar to those of Saint Lucia.

Effect of some high consumption spices on hemoglobin glycation.

Science.gov (United States)

Naderi, G H; Dinani, Narges J; Asgary, S; Taher, M; Nikkhoo, N; Boshtam, M

2014-01-01

Formation of glycation products is major factor responsible in complications of diabetes. Worldwide trend is toward the use of natural additives in reducing the complications of diseases. Therefore, there is a growing interest in natural antiglycation found in plants. Herbs and spices are one of the most important targets to search for natural antiglycation from the point of view of safety. This study investigated the ability of some of the spices to inhibit glycation process in a hemoglobin/glucose model system and compared their potency with each other. For this subject the best concentration and time to incubate glucose with hemoglobin was investigated. Then the glycosylation degree of hemoglobin in the presence of extracts by the three concentrations 0.25, 0.5 and 1 μg/ml was measured colorimetrically at 520 nm. Results represent that some of extracts such as wild caraway, turmeric, cardamom and black pepper have inhibitory effects on hemoglobin glycation. But some of the extracts such as anise and saffron have not only inhibitory effects but also aggravated this event and have proglycation properties. In accordance with the results obtained we can conclude that wild caraway, turmeric, cardamom and black pepper especially wild caraway extracts are potent antiglycation agents, which can be of great value in the preventive glycation-associated complications in diabetes.

Adult, embryonic and fetal hemoglobin are expressed in human glioblastoma cells.

Science.gov (United States)

Emara, Marwan; Turner, A Robert; Allalunis-Turner, Joan

2014-02-01

Hemoglobin is a hemoprotein, produced mainly in erythrocytes circulating in the blood. However, non-erythroid hemoglobins have been previously reported in other cell types including human and rodent neurons of embryonic and adult brain, but not astrocytes and oligodendrocytes. Human glioblastoma multiforme (GBM) is the most aggressive tumor among gliomas. However, despite extensive basic and clinical research studies on GBM cells, little is known about glial defence mechanisms that allow these cells to survive and resist various types of treatment. We have shown previously that the newest members of vertebrate globin family, neuroglobin (Ngb) and cytoglobin (Cygb), are expressed in human GBM cells. In this study, we sought to determine whether hemoglobin is also expressed in GBM cells. Conventional RT-PCR, DNA sequencing, western blot analysis, mass spectrometry and fluorescence microscopy were used to investigate globin expression in GBM cell lines (M006x, M059J, M059K, M010b, U87R and U87T) that have unique characteristics in terms of tumor invasion and response to radiotherapy and hypoxia. The data showed that α, β, γ, δ, ζ and ε globins are expressed in all tested GBM cell lines. To our knowledge, we are the first to report expression of fetal, embryonic and adult hemoglobin in GBM cells under normal physiological conditions that may suggest an undefined function of those expressed hemoglobins. Together with our previous reports on globins (Ngb and Cygb) expression in GBM cells, the expression of different hemoglobins may constitute a part of series of active defence mechanisms supporting these cells to resist various types of treatments including chemotherapy and radiotherapy.

Comparison of the gold standard of hemoglobin measurement with the clinical standard (BGA) and noninvasive hemoglobin measurement (SpHb) in small children: a prospective diagnostic observational study.

Science.gov (United States)

Wittenmeier, Eva; Bellosevich, Sophia; Mauff, Susanne; Schmidtmann, Irene; Eli, Michael; Pestel, Gunther; Noppens, Ruediger R

2015-10-01

Collecting a blood sample is usually necessary to measure hemoglobin levels in children. Especially in small children, noninvasively measuring the hemoglobin level could be extraordinarily helpful, but its precision and accuracy in the clinical environment remain unclear. In this study, noninvasive hemoglobin measurement and blood gas analysis were compared to hemoglobin measurement in a clinical laboratory. In 60 healthy preoperative children (0.2-7.6 years old), hemoglobin was measured using a noninvasive method (SpHb; Radical-7 Pulse Co-Oximeter), a blood gas analyzer (clinical standard, BGAHb; ABL 800 Flex), and a laboratory hematology analyzer (reference method, labHb; Siemens Advia). Agreement between the results was assessed by Bland-Altman analysis and by determining the percentage of outliers. Sixty SpHb measurements, 60 labHb measurements, and 59 BGAHb measurements were evaluated. In 38% of the children, the location of the SpHb sensor had to be changed more than twice for the signal quality to be sufficient. The bias/limits of agreement between SpHb and labHb were -0.65/-3.4 to 2.1 g·dl(-1) . Forty-four percent of the SpHb values differed from the reference value by more than 1 g·dl(-1) . Age, difficulty of measurement, and the perfusion index (PI) had no influence on the accuracy of SpHb. The bias/limits of agreement between BGAHb and labHb were 1.14/-1.6 to 3.9 g·dl(-1) . Furthermore, 66% of the BGAHb values differed from the reference values by more than 1 g·dl(-1) . The absolute mean difference between SpHb and labHb (1.1 g·dl(-1) ) was smaller than the absolute mean difference between BGAHb and labHb (1.5 g·dl(-1) /P = 0.024). Noninvasive measurement of hemoglobin agrees more with the reference method than the measurement of hemoglobin using a blood gas analyzer. However, both methods can show clinically relevant differences from the reference method (ClinicalTrials.gov: NCT01693016). © 2015 John Wiley & Sons Ltd.

Whose Knowledge, Whose Values? The Contribution of Local Knowledge to Education Policy Processes: A Case Study of Research Development Initiatives in the Small State of Saint Lucia

Science.gov (United States)

Holmes, Keith; Crossley, Michael

2004-01-01

This paper draws upon a case study of education in the small Caribbean state of Saint Lucia (population 154,000) to examine how local knowledge and values can influence the education policy process. It is argued that recent research development initiatives have strengthened the ability of Saint Lucia to mediate international education agendas to…

Clinical Significance of Reticulocyte Hemoglobin Content in the Diagnosis of Iron Deficiency Anemia

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Mustafa Karagülle

2013-06-01

Full Text Available OBJECTIVE: The aim of this study was to evaluate the clinical significance of reticulocyte hemoglobin content (CHr in the diagnosis of iron deficiency anemia (IDA and to compare it with other conventional iron parameters. METHODS: A total of 32 female patients with IDA (serum hemoglobin 120 g/L and serum ferritin <20 ng/mL were enrolled. RESULTS: CHr was 24.95±3.92 pg in female patients with IDA and 29.93±2.96 pg in female patients with iron deficiency. CHr showed a significant positive correlation with hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, serum iron, and transferrin saturation and a significant negative correlation with transferrin and total iron-binding capacity. The cut-off value of CHr for detecting IDA was 29 pg. CONCLUSION: Our data demonstrate that CHr is a useful parameter that can be confidently used in the diagnosis of IDA, and a CHr cut-off value of 29 pg predicts IDA.

The subunit structure of the extracellular hemoglobin of Biomphalaria glabrata

International Nuclear Information System (INIS)

Arndt, Marcio H.L.; Naves, Cristiani F.; Xavier, Luciana P.; Santoro, Marcelo M.

1997-01-01

Full text. The hemoglobin of Biomphalaria glabrata was purified to homogeneity by a two step purification protocol using a gel filtration column (Superose 6 HR/Pharmacia ) followed by an anion exchange chromatography (MONO-Q Sepharose/Pharmacia). The dissociation products were analysed by a 5 - 15 % Polyacrylamide gel electrophoresis containing Sodium Dodecyl Sulfate (SDS-PAGE) giving a band of 270 K Daltons and a band of 180 K Daltons after reduction with β-mercaptoethanol. The same profile was obtained in a 3.5 % Agarose gel electrophoresis containing SDS (SDS-AGE) showing additional bands of higher molecular weight. These bands were proposed to be monomers, dimers and trimers and, after reduction in a Bidimensional SDS-AGE, the proposed monomers and dimers were decomposed in two and four bands that were interpreted as 1 - 4 chains. The hemoglobin was digested by four different proteases ( Thrombin, Trypsin, Chymotrypsin and Subtilisin ) showing several equivalent fragments with molecular weights multiples of its minimum molecular weight ( 17.7 K Daltons). The circular dichroism spectrum of the protein showed a characteristic high α-helix content. We proposed that this hemoglobin is a pentamer of approx. 360 K Daltons subunits each formed by two 180 K Daltons chains linked in pairs by disulfide bridges and each of these chains comprises ten Heme binding domains. These data were compared to other Planorbidae extracellular hemoglobins. Up to now, the quaternary structure of this hemoglobin (shape and disposition of the subunits) is unknown. It is intended to elucidate its structure by Small Angle X-Ray Scattering in Brazilian National Laboratory of Synchrotron Light (LNLS). (author)

Geographic distribution of ebony leaf monkey Trachypithecus auratus (E. Geoffroy Saint-Hilaire, 1812) (Mammalia: Primates: Cercopithecidae)

NARCIS (Netherlands)

Nijman, Vincent

2000-01-01

As one of the fundamental units of ecology and biogeography, the geographic distribution of the endemic and threatened ebony leaf monkey Trachypithecus auratus (E. Geoffroy Saint-Hilaire, 1812) on the islands of Java, Bali, and Lombok (Indonesia) has been assessed. All localities where the species

Postoperative hemoglobin level in patients with femoral neck fracture

OpenAIRE

Nagra, Navraj; van Popta, Dmitri; Whiteside, Sigrid; Holt, Edward

2018-01-01

Objective: The aim of this study was to analyze the changes of hemoglobin levels in patients undergoing fixation for femoral neck fracture.Methods: Peroperative hemoglobin levels of patients who underwent either dynamic hip screw (DHS) fixation (n=74; mean age: 80 years) or hip hemiarthroplasty (n=104; mean age: 84 years) for femoral neck fracture was monitored.Results: There was a statistically and clinically significant mean drop of 31.1 g/L between the preoperative (D0) and postoperative D...

Direct electrochemistry and electrocatalysis of hemoglobin protein entrapped in graphene and chitosan composite film.

Science.gov (United States)

Xu, Huifeng; Dai, Hong; Chen, Guonan

2010-04-15

A novel, biocompatible sensing strategy based on graphene and chitosan composite film for immobilizing the hemoglobin protein was firstly adopted. The direct electron transfer and bioelectrocatalytic activity of hemoglobin after incorporation into the composite film were investigated. A pair of reversible redox waves of hemoglobin was appeared, and hemoglobin could exhibit its bioelectrocatalytic activity toward H(2)O(2) in a long term. Such results indicated that graphene and chitosan composite could be a friendly biocompatible interface for immobilizing biomolecules and keeping their native structure. Furthermore, the appearance of graphene in the composite film could facilitate the electron transfer between matrix and the electroactive center of hemoglobin. Hence, this graphene and chitosan based protocol would be a promising platform for protein immobilization and biosensor preparation. (c) 2010 Elsevier B.V. All rights reserved.

Center for Transportation Studies 24th annual transportation research conference, May 22-23, 2013, Saint Paul, Minnesota.

Science.gov (United States)

2013-05-01

The University of Minnesotas Center for Transportation : Studies is pleased to present its 24th Annual Transportation : Research Conference, May 22-23, 2013. The conference will : be held at the Saint Paul RiverCentre, 175 West Kellogg : Boulevard...

A study on the structures of hemoglobin of diabetic patients by EXAFS technique

International Nuclear Information System (INIS)

Wang Yinsong; Tan Mingguang; Zhang Guilin

2001-01-01

Hemoglobin was the carrier of oxygen in blood circulation. For the patients with diabetes mellitus the enhancement of glycidate hemoglobin in blood causes the decrease of oxygen transmission function. The local atomic structures of iron in hemoglobin were determined by EXAFS techniques. The relationship between diabetes mellitus and hemoglobin structures was observed. The blood samples were taken from normal people, slight and serious diabetic patients. The results show that the coordination number of iron atoms and Fe-O bond length were almost the same for the three samples. However, for the samples of serious diabetic patients the Fe-N bond length increases by about 0.002 nm, the possible reasons were the increase of deoxyhemoglobin contents in their blood

COMPARISON OF FRUCTOSAMINE AND GLYCOSYLATED HEMOGLOBIN IN A NON-INSULIN DEPENDENT DIABETIC POPULATION

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M. Amini

1999-08-01

Full Text Available In an attempt to determine the clinical value of frnctosamine assay for monitoring type II diabetic patients, correlation of frnctosamine with glycosylated hemoglobin was studied. 100 patients with type II diabetes mcllitus were compared with 100 normal subjects. Fasting blood glucose, glycosylated hemoglobin, albumin and frnctosamine were measured in alt subjects. In the diabetic patients, a significant correlation was observed between fasting blood glucose and glycosylated hemoglobin (r = 0.64, p < 0.01 and scrum frnctosamine (r = 0.7, P < 0.01. Tlicrc was also a significant correlation between glycosylated hemoglobin and scrum frtictosmine (r = .94, I'<0.01. Frnctosamine, assay can be used as an index of diabetes control.

Action of radiation on biosynthesis of hemoglobin and some of its electrophoretic fractions

International Nuclear Information System (INIS)

Starodub, N.F.; Kriklivyj, I.A.; Shur'yan, I.M.

1976-01-01

Biosynthesis of hemoglobin and some of its electrophoretic fractions in red cells of peripheral blood and spleen of irradiated (650 R) rats has been studied. Hemoglobin synthesis is found to be most drastically inhibited in the first and second fractions on the first and eighth days after irradiation and in the fifth and sixth fractions on the eighth day (less expressed). The synthesis is restored on the twelfth day, the process under study proceeding more slowly in the above-mentioned fractions than in others. In the course of radiation sickness, the biosynthesis of certain hemoglobin fractions varies differently in the hemoglobin-synthesizing cells of peripheral blood than in the cells of spleenic erythroid series

Hemoglobin A1c (HbA1c) Test: MedlinePlus Lab Test Information

Science.gov (United States)

... page: https://medlineplus.gov/labtests/hemoglobina1chba1ctest.html Hemoglobin A1c (HbA1c) Test To use the sharing features on this page, please enable JavaScript. What is a hemoglobin A1c (HbA1c) test? A hemoglobin A1c (HbA1c) test measures ...

Immediate response of the hemoglobin system of the goldfish, Carassius auratu, to temperature change

Energy Technology Data Exchange (ETDEWEB)

Houston, A.H.; Rupert, R.

1976-10-01

Goldfish acclimated to 3 and 23/sup 0/C were characterized by two- and three-component hemoglobin systems, respectively. After acclimation to a diurnally cycling temperature regime (approximately 3 to approximately 23/sup 0/C), specimens sampled at approximately 23/sup 0/C and approximately 3/sup 0/C were identical in terms of hemoglobin system complexity with those held at equivalent constant temperatures. Abrupt transfer of fish acclimated at constant 23/sup 0/C to 3/sup 0/C, and vice versa, lead to appearance or disappearance of the minor component, G. l, within 3 h. In vitro cooling and warming of whole blood and hemolyzate samples indicated that hemoglobin system modification occurred under cell-free as well as cell-intact conditions. These observations suggest that previously observed quantitative variations in the hemoglobin systems of thermally acclimated teleosts may represent, in part at least, altered aggregation of preexisting subunits rather than de novo hemoglobin synthesis and raise the possibility that teleostean hemoglobin systems may possess a capacity for rapid, adaptative reorganization after environmental temperature variation.

Nutrition education and knowledge, attitude and hemoglobin status of Malaysian adolescents.

Science.gov (United States)

Yusoff, Hafzan; Daud, Wan Nudri Wan; Ahmad, Zulkifli

2012-01-01

A higher occurrence of iron deficiency anemia is present in rural Malaysia than urban Malaysia due to a lower socio-economic status of rural residents. This study was conducted in Tanah Merah, a rural district of Kelantan, Malaysia. Our objective was to investigate the impact of nutrition education alone, daily iron, folate and vitamin C supplementation or both on knowledge, attitudes and hemoglobin status of adolescent students. Two hundred eighty fourth year secondary students were each assigned by school to 1 of 4 different treatment groups. Each intervention was carried out for 3 months followed by 3 months without treatment. A validated self-reported knowledge and attitude questionnaire was administered; hemoglobin levels were measured before and after intervention. At baseline, no significant difference in hemoglobin was noted among the 4 groups (p = 0.06). The changes in hemoglobin levels at 3 months were 11, 4.6, 3.9 and -3.7% for the supplementation, nutrition education, combination and control groups, respectively. The changes at 6 months were 1.0, 6.8, 3.7 and -14.8%, respectively. Significant improvements in knowledge and attitude were evidenced in both the nutritional education and combination groups. The supplementation and control groups had no improvement in knowledge or attitudes. This study suggests nutritional education increases knowledge, attitudes and hemoglobin levels among Malaysian secondary school adolescents.

A Comparative Case Study of Self-Actualization in Eleanor Roosevelt and Antoine de Saint-Exupery.

Science.gov (United States)

Tyska, Cynthia Ann

Eleanor Roosevelt and Antoine de Saint-Exupery are described as strongly developed self-actualizing people. They were selected as subjects of this study because they are generally believed to possess self-actualizing characteristics and because their positions as public figures made it more likely that data on them would be accessible.…

Fluorescent analysis of interaction of flavonols with hemoglobin and bovine serum albumin

Science.gov (United States)

Sentchouk, V. V.; Bondaryuk, E. V.

2007-09-01

We have studied the fluorescent properties of flavonols (quercetin, fisetin, morin, rutin) with the aim of studying possible interaction with hemoglobin and bovine serum albumin (BSA). We observed an increase in the intensity of intrinsic fluorescence for all the flavonols except rutin in the presence of BSA. From the changes in the fluorescence spectra, we concluded that tautomeric forms are formed on interaction with hemoglobin. We determined the interconnection between the structure of related flavonols and their fluorescent properties on interaction with proteins, and we determined the binding constants for binding with BSA and hemoglobin.

Estética del 'cross a la mandíbula' y elementos modernista - decadentes en Roberto Arlt

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Laura Susana Juárez

2003-11-01

Full Text Available El trabajo reflexiona sobre el cruce de tendencias presentes en lo que se ha considerado como la primera etapa de la obra de Roberto Arlt. Así, en la estética arltiana de una literatura que encierra “la viol encia de un ‘cross’ a la mandíbula”, definida en las palabras preliminares a Los lanzallamas, se introducen algunos rasgos que provienen del modernismo y del imaginario decadente, en muchos aspectos, muy criticados por el propio escritor. En este marco, se analiza, en primer lugar, “Jehová” (1918, el primer cuento que publicó, cuya construcción evidencia lecturas de fuerte matriz modernista. Luego, se estudia el modo en que Arlt intenta separarse del modernismo-decadentismo en sus textos programáticos, sus novelas y sus primeras narraciones, y la forma en que, de todas maneras, se retoman en su literatura ciertos elementos que remiten visiblemente a la estética en cuestión. Finalmente, indagamos cómo, cuando en Arlt puede observarse una pretensión de “estilo alto”, aparece el modernismo

Comparison of Hemoglobin Levels Before and After Hemodialysis and Their Effects on Erythropoietin Dosing and Cost

OpenAIRE

Sagheb; Fallahzadeh; Moaref; Fallahzadeh; Dormanesh

2016-01-01

Background Hemoglobin levels measured after hemodialysis, as compared to hemoglobin levels measured before hemodialysis, are suggested to be a more accurate reflection of the hemoglobin levels between hemodialysis sessions, and to be a better reference point for adjusting erythropoietin dosing. Objectives The aim of this study was to compare the hemoglobin levels before and after hemodialysis, to calculate the required erythropoie...

Hemoglobin Q-Iran detected in family members from Northern Iran: a case report

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Khorshidi Mohammad

2012-02-01

Full Text Available Abstract Introduction Hemoglobin Q-Iran (α75Asp→His is an important member of the hemoglobin Q family, molecularly characterized by the replacement of aspartic acid by histidine. The first report of hemoglobin Q-Iran and the nomenclature of this hemoglobinopathy dates back to 1970. Iran is known as a country with a high prevalence of α- and β-thalassemia and different types of hemoglobinopathy. Many of these variants are yet to be identified as the practice of molecular laboratory techniques is limited in this part of the world. Applying such molecular methods, we report the first hemoglobin Q-Iran cases in Northern Iran. Case presentation An unusual band was detected in an isoelectric focusing test and cellulose acetate electrophoresis of a sample from a 22-year-old Iranian man from Mazandaran Province. Capillary zone electrophoresis analysis identified this band as hemoglobin Q. A similar band was also detected in his mother's electrophoresis (38 years, Iranian ethnicity. The cases underwent molecular investigation and the presence of a hemoglobin Q-Iran mutation was confirmed by the amplification refractory mutation system polymerase chain reaction method. Direct conventional sequencing revealed a single guanine to cytosine missense mutation (c.226G > C; GAC >CAC at codon 75 in the α-globin gene in both cases. Conclusion The wide spectrum and high frequency of nondeletional α-globin mutations in Mazandaran Province is remarkable and seem to differ considerably from what has been found in Mediterranean populations. This short communication reports the first cases of patients with hemoglobin Q found in that region.

76 FR 555 - Keystone Holdings, LLC and Compagnie de Saint-Gobain; Analysis of Proposed Agreement Containing...

Science.gov (United States)

2011-01-05

... Asia for a purchase price of $245 million. As originally structured, the assets acquired by Keystone... future, or that Saint-Gobain could sell the business upon terms that would reduce competition in the North American alumina wear tile markets. Therefore, the proposed Consent Agreement requires that...

Multiple sclerosis in Latter Day Saints (Mormons).

Science.gov (United States)

Hawkes, C H; Ramkumar, N; Baker, R; Lyon, J L

2007-04-01

Compliant members of the Church of Jesus Christ of Latter Day Saints (LDS, Mormons) have a low incidence of heart and lung disease that may relate to their healthy life style. We wished to determine whether multiple sclerosis (MS) was less frequent in this religious body. To ascertain this, diagnostic and treatment coding records were accessed from the Deseret Mutual Benefit Administrators (DMBA) for the 6 year period 1997-2002. DMBA is a medical insurance company that provides medical insurance to all employees of LDS Church in the US. This information was combined with prescribing records for disease modifying treatment, principally beta-interferon and Copaxone which are medications specific to MS. Using various search strategies we derived an approximate MS prevalence of 45-64/100,000. Comparison with MS rates from Utah and other states of comparable latitude suggest that strict LDS have an MS prevalence that is lower than expected and may reflect their healthy life style.

Saint Venant's equation and theory of characteristics

International Nuclear Information System (INIS)

Daubert, Andre

1978-01-01

This theory, in its general scope, will be dealt with through the concrete example of Saint Venant's equations which govern the waves in channels. 1. Finding the characteristic directions. The aim is to interpret the hyperbolic sort of equations to show that there is a way of combining them in order to shape them so that they express a linear relation between the variations of the unknowns when moving along particular differential paths. In certain cases, this differential relation can integrate to lead to Rieman's invariants. 2. Relation between the theory of characteristics and the wave equation. In the linear systems case, it is worthwhile showing simply, how the method of characteristics is linked to the conventional treatment of the wave equation. 3. Relation between the theory of characteristics and the Cauchy problem. The theory of characteristics is frequently introduced as from the Cauchy problem, the characteristics forming the conditions of indetermination of the Cauchy problem [fr

γ irradiation of aqueous solutions of human hemoglobin in atmospheres of air and argon

International Nuclear Information System (INIS)

Puchala, M.; Szweda-Lewandowska, Z.; Leyko, W.

1979-01-01

In this study, the degrees of destruction of hemoglobin irradiated in atmospheres of air and argon were compared. Hemoglobin preparations were irradiated in the forms: oxyhemoglobin (HbO 2 ) deoxyhemoglobin (Hb 2+ ) and methemoglobin (MetHb) applying doses of 0.5 to 5 Mrad. The degree of hemoglobin destruction was estimate on the basis of changes in the values of the absorption coefficient at the Soret band, the absorption ratio A 505 /A 563 determined after conversion of irradiated preparations into MetHb, absorption coefficinets for pyridine hemochromogen obtained from irradiated preparations, and changes in parameters characterizing the hemoglobin oxygenation reaction (log p/sub 1/2/O 2 and the Hill n coefficient). The calculated oxygen enhancement ratios S were generally higher than 1 for the parameters estimated. This indicates that the presence of oxygen during irradiation enhances hemoglobin destruction

Serum-free Erythroid Differentiation for Efficient Genetic Modification and High-Level Adult Hemoglobin Production.

Science.gov (United States)

Uchida, Naoya; Demirci, Selami; Haro-Mora, Juan J; Fujita, Atsushi; Raines, Lydia N; Hsieh, Matthew M; Tisdale, John F

2018-06-15

In vitro erythroid differentiation from primary human cells is valuable to develop genetic strategies for hemoglobin disorders. However, current erythroid differentiation methods are encumbered by modest transduction rates and high baseline fetal hemoglobin production. In this study, we sought to improve both genetic modification and hemoglobin production among human erythroid cells in vitro . To model therapeutic strategies, we transduced human CD34 + cells and peripheral blood mononuclear cells (PBMCs) with lentiviral vectors and compared erythropoietin-based erythroid differentiation using fetal-bovine-serum-containing media and serum-free media. We observed more efficient transduction (85%-93%) in serum-free media than serum-containing media (20%-69%), whereas the addition of knockout serum replacement (KSR) was required for serum-free media to promote efficient erythroid differentiation (96%). High-level adult hemoglobin production detectable by electrophoresis was achieved using serum-free media similar to serum-containing media. Importantly, low fetal hemoglobin production was observed in the optimized serum-free media. Using KSR-containing, serum-free erythroid differentiation media, therapeutic adult hemoglobin production was detected at protein levels with β-globin lentiviral transduction in both CD34 + cells and PBMCs from sickle cell disease subjects. Our in vitro erythroid differentiation system provides a practical evaluation platform for adult hemoglobin production among human erythroid cells following genetic manipulation.

Correlation between Oxygen Saturation and Hemoglobin and Hematokrit Levels in Tetralogy of Fallot Patients

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Farhatul Inayah Adiputri

2016-03-01

Full Text Available Background: Hemoglobin and hematocrit levels increase in Tetralogy of Fallot (TOF but the oxygen saturation declines. Reduced hemoglobin in circulating blood as a parameter of cyanosis does not indicate rising hemoglobin due to the ‘not-working’ hemoglobins that affect the oxygen saturation. Increasing hematocrit is the result of secondary erythrocytosis caused by declining oxygen level in blood, which is related to the oxygen saturation. This study was conducted to find the correlation between oxygen saturation and hemoglobin and hematocrite levels in TOF patients. Methods: This study was undertaken at Dr. Hasan Sadikin General Hospital in the period of January 2011 to December 2012 using the cross-sectional analytic method with total sampling technique. Inclusion criteria were medical records of TOF patients diagnosed based on echocardiography that included data on oxygen saturation, hemoglobin, and hematocrite. Exclusion criteria was the history of red blood transfusion. Results: Thirty medical records of TOF patiens from Dr. Hasan Sadikin General Hospital Bandung were included in this study. Due to skewed data distribution, Spearman correlation test was used to analyze the data. There was a significant negative correlation between oxygen saturation and hematocrit level (r= -0.412; p=0.024 and insignificant correlation between oxygen saturation and hemoglobin (r=-0.329; p= 0.076. Conclusions: There is a weak negative correlation between oxygen saturation and hematocrite levels

Using the Cascade Model to Improve Antenatal Screening for the Hemoglobin Disorders

Science.gov (United States)

Gould, Dinah; Papadopoulos, Irena; Kelly, Daniel

2012-01-01

Introduction: The inherited hemoglobin disorders constitute a major public health problem. Facilitators (experienced hemoglobin counselors) were trained to deliver knowledge and skills to "frontline" practitioners to enable them to support parents during antenatal screening via a cascade (train-the-trainer) model. Objectives of…

Patterns of glycemic control using glycosylated hemoglobin in diabetics.

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Kahlon, Arunpreet Singh; Pathak, Rambha

2011-07-01

Till now estimation of blood glucose is the highly effective method for diagnosing diabetes mellitus but it provides a short-term picture of control. More evidence is required to prove that plasma glucose and glycosylated hemoglobin levels together gives a better estimate of glycemic control and compliance with treatment. Indian diabetes risk score (IDRS) is a simplified screening tool for identifying undiagnosed diabetic subjects, requires minimum time, and effort and can help to considerably reduce the costs of screening. To study patterns of glycemic control using glycosylated hemoglobin in diabetic patients. To find out correlation between levels of plasma glucose and glycosylated hemoglobin in diabetics and to calculate IDRS of the study population. A cross sectional study was conducted among 300 known diabetic patients attending outpatient department of a rural medical college in Haryana, India. Following standard procedures and protocols FPG and glycosylated hemoglobin were measured to find out a pattern of glycemic control in them after taking their written and informed consent. A correlation between the levels of glycosylated hemoglobin and fasting blood glucose was also calculated. These patients were made to fill a performa and their demographic and clinical risk factors were noted and based on this, their IDRS was calculated. This was done to validate the IDRS in Indian rural population. Fifty-two percent of the population had fasting plasma glucose level between 125-150 mg/dl, 21% had this level between 151-175 mg/dl. Thirteen percent of the study subjects had HbA1C between 6.5-7.5, more than half (57.3%) had this value between 7.5-8.5, 12% and 18% had values between 8.5-9.5 and 9.5-10.5, respectively. Twelve percent of the participants had HbA1C level higher than 10.5. Correlation of fasting plasma glucose level and HbA1C was also studied and found that correlation coefficient came out to be .311. This correlation was found to be statistically

High-rise construction in the Saint Petersburg agglomeration in 1703–1950s

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Sementsov Sergey

2018-01-01

Full Text Available Regularities of high-rise construction (implemented projects and developments in Saint Petersburg and the Saint Petersburg agglomeration since the foundation of the city in 1703 till the 1950s are considered. Based on these regularities, a single spatially developed system of vertical dominants is formed. High-rise construction in the city and its suburbs started in the 1710s and continues up to the present time. In the considered decades (1703–1950s, high-rise construction mostly performed urban-planning functions (with vertical and symbolic dominants, relying on patterns of the visual perception of man-made landscapes under development. Since the 1710s, the construction of vertical dominants (mainly temples, spires of towers, lighthouses, etc. of five ranks (depending on the altitude range and in relation to the background development was conducted in territories of the entire agglomeration. These dominants were arranged in landscapes of the city and suburbs with almost mathematically precise accuracy and according to special regulations. Such dominants obtained particular descriptive and silhouette characteristics in accordance with the conditions of spatial perception. In some periods of city development, attempts were made to create monuments (symbolic dominants of specific height and include those in the spatial system of high-rise dominants as significant elements of the city silhouette.

Anemia and hemoglobin levels among Indigenous Xavante children, Central Brazil.

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Ferreira, Aline Alves; Santos, Ricardo Ventura; Souza, July Anne Mendonça de; Welch, James R; Coimbra, Carlos E A

2017-01-01

To evaluate the prevalence of anemia, mean hemoglobin levels, and the main nutritional, demographic, and socioeconomic factors among Xavante children in Mato Grosso State, Brazil. A survey was conducted with children under 10 years of age in two indigenous Xavante communities within the Pimentel Barbosa Indigenous Reserve. Hemoglobin concentration levels, anthropometric measurements, and socioeconomic/demographic data were collected by means of clinical measurements and structured interviews. The cut-off points recommended by the World Health Organization were used for anemia classification. Linear regression analyses with hemoglobin as the outcome and Poisson regression with robust variance and with the presence or absence of anemia as outcomes were performed (95%CI). Lower mean hemoglobin values were observed in children under 2 years of age, without a significant difference between sexes. Anemia was observed among 50.8% of children overall, with the highest prevalence among children under 2 years of age (77.8%). Age of the child was inversely associated with the occurrence of anemia (adjusted PR = 0.60; 95%CI 0.38-0.95) and mean hemoglobin values increased significantly with age. Greater height-for-age z-score values reduced the probability of having anemia by 1.8 times (adjusted PR = 0.59; 95%CI 0.34-1.00). Presence of another child with anemia within the household increased the probability of the occurrence of anemia by 52.9% (adjusted PR = 1.89; 95%CI 1.16-3.09). Elevated levels of anemia among Xavante children reveal a disparity between this Indigenous population and the national Brazilian population. Results suggest that anemia is determined by complex and variable relationships between socioeconomic, sociodemographic, and biological factors.

La vision de Saint Eustathe dans la peinture post-byzantine

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Amprazogoula Katerina

2008-01-01

Full Text Available (francuski L'article étudie l'évolution de la Vision de saint Eustathe dans une série de peintures murales et d'icônes portatives - allant du XVIe au XVIIIe siècle - les formules iconographiques adoptées et leurs particularités ainsi que la place et la fonction de cette image dans la peinture murale. Le matériel étudié a montré une diversité de formules iconographiques qui exploitent pour l'essentiel la tradition byzantine et orientale du sujet, en manifestant en même temps ses propres traits ainsi que l'influence des images occidentales. .

Correlation of Oxygenated Hemoglobin Concentration and Psychophysical Amount on Speech Recognition

Science.gov (United States)

Nozawa, Akio; Ide, Hideto

The subjective understanding on oral language understanding task is quantitatively evaluated by the fluctuation of oxygenated hemoglobin concentration measured by the near-infrared spectroscopy. The English listening comprehension test wihch consists of two difficulty level was executed by 4 subjects during the measurement. A significant correlation was found between the subjective understanding and the fluctuation of oxygenated hemoglobin concentration.

The Reaction of Oxy Hemoglobin with Nitrite

DEFF Research Database (Denmark)

Hathazi, Denisa; Scurtu, Florina; Bischin, Cristina

2018-01-01

The autocatalytic reaction between nitrite and the oxy form of globins involves free radicals. For myoglobin (Mb), an initial binding of nitrite to the iron-coordinated oxygen molecule was proposed; the resulting ferrous-peroxynitrate species was not detected, but its decay product, the high...... to a simple kinetic model involving a transient met-aqua form, in contrast to the ferryl detected in the case of Mb in a similar reaction sequence. These data are in line with a previous observation of a transient accumulation of ferryl Hb under auto-catalytic conditions at much lower concentrations......-peroxynitrate. Density functional theory (DFT) calculations support this latter assignment. The reaction allows for differentiating between the reactivities of various chemically modified hemoglobins, including candidates for blood substitutes. Polymerization of hemoglobin slows the nitrite-induced oxidation, in sharp...

Hemoglobin promotes somatic embryogenesis in peanut cultures.

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Jayabalan, N; Anthony, P; Davey, M R; Power, J B; Lowe, K C

2004-02-01

Critical parameters influencing somatic embryogenesis include growth regulators and oxygen supply. Consequently, the present investigation has focused on optimization of a somatic embryogenic system for peanut (Arachis hypogaea L.) through media supplementation with the auxin, picloram. The latter at 30 mg L(-1) was optimal for inducing regeneration of somatic embryos from cultured explants of zygotic embryos. In contrast, somatic embryogenesis did not occur in the absence of this growth regulator. An assessment has also been made of the beneficial effect on somatic embryogenesis and plant regeneration of the commercial hemoglobin (Hb) solution, Erythrogen. Hemoglobin at 1:50 and 1:100 (v:v) stimulated increases in mean fresh weight (up to a maximum of 57% over control), mean number of explants producing somatic embryos (15%) and mean number of somatic embryos per explant (29%).

Study of the interaction between bovine hemoglobin and analogs of biphenyldicarboxylate by spectrofluorimetry

International Nuclear Information System (INIS)

Wang, Ruiyong; Yin, Yujing; Wang, Ruiqiang; Xie, Yuanzhe; Ge, Baoyu; Li, Zhigang; Li, Zhen; Shi, Jie; Chang, Junbiao

2013-01-01

The interaction between bovine hemoglobin and analogs of Biphenyldicarboxylate was investigated by fluorescence, synchronous fluorescence, ultraviolet–vis absorbance, resonance light-scattering spectra and three-dimensional fluorescence spectra at pH 7.40. The quenching mechanism and binding constants were determined by the quenching of bovine hemoglobin fluorescence in presence of analogs. Results showed that the nature of the quenching was of static type. Both the van der Waals and hydrogen bonding played a major role in stabilizing the complex. The distance between donor and acceptors was obtained to be 2.11–2.25 nm according to Förster's theory. The influence of analogs on the conformation of bovine hemoglobin was investigated. -- Highlights: • The interactions between bovine hemoglobin and analogs of DDB have been investigated. • Results reveal that DDB has the strongest affinity for hemoglobin among four compounds. • The van der Waals and hydrogen bonding play major role in the binding process. • The influence of molecular structure on the binding aspects has been investigated

HbA1c levels in individuals heterozygous for hemoglobin variants.

Science.gov (United States)

Tavares, Ricardo Silva; Souza, Fábio Oliveira de; Francescantonio, Isabel Cristina Carvalho Medeiros; Soares, Weslley Carvalho; Mesquita, Mauro Meira

2017-04-01

To evaluate the levels of glycated hemoglobin (HbA1c) in patients heterozygous for hemoglobin variants and compare the results of this test with those of a control group. This was an experimental study based on the comparison of HbA1c tests in two different populations, with a test group represented by individuals heterozygous for hemoglobin variants (AS and AC) and a control group consisting of people with electrophoretic profile AA. The two populations were required to meet the following inclusion criteria: Normal levels of fasting glucose, hemoglobin, urea and triglycerides, bilirubin > 20 mg/dL and non-use of acetylsalicylic acid. 50 heterozygous subjects and 50 controls were evaluated between August 2013 and May 2014. The comparison of HbA1c levels between heterozygous individuals and control subjects was performed based on standard deviation, mean and G-Test. The study assessed a test group and a control group, both with 39 adults and 11 children. The mean among heterozygous adults for HbA1c was 5.0%, while the control group showed a rate of 5.74%. Heterozygous children presented mean HbA1c at 5.11%, while the controls were at 5.78%. G-Test yielded p=0.93 for children and p=0.89 for adults. Our study evaluated HbA1c using ion exchange chromatography resins, and the patients heterozygous for hemoglobin variants showed no significant difference from the control group.

FOOLISHNESS FOR CHRIST, LOVE AND MATERNITY IN LUDMILA ULITSKAYA'S PLAY "THE SEVEN SAINTS"

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Moteyunaite I. V.

2008-11-01

Full Text Available The article presents a new understanding of such a complex cultural phenomenon as foolishness for Christ through the example of Ludmila Ulitskaya's historical play "The Seven Saints". The author shows that the hero of the 1990's became a blessed woman (a God's fool, who neither sets an optimistic example nor gives faith in the triumph of spirit, but who can only die with dignity, following Christ's way and His final sacrifice.

Hemoglobin concentrations and associated factors in adolescentes from Recife, Brazil

OpenAIRE

Elisângela Barros Soares Mendonça; Lilian Ferreira Muniz; Ilma Kruze Grande de Arruda; Alcides da Silva Diniz

2014-01-01

OBJECTIVE: To estimate the prevalence of anemia and associated factors in adolescents from the city of Recife in Pernambuco state. METHODS: This is a cross-sectional study, involving a random sample of 256 adolescents of both genders, aged 13 to 18, whose hemoglobin concentrations were evaluated, along with their nutritional status and socioeconomic and demographic characteristics. RESULTS: The prevalence of inadequate hemoglobin concentrations was 10.2% [CI95%=6.7-14.5], reaching levels cons...

EPR studies of cooperative binding of Cu (II) to hemoglobin

International Nuclear Information System (INIS)

Louro, S.R.W.; Tabak, M.

1983-07-01

The investigation of the relative affinities of the two pairs of hemoglobin copper sites by monitoring the EPR spectra of the complexes formed by the reaction of copper with deoxyhemoglobin is reported. A model in which two sites are assumed to accept copper ions in a noncooperative way is not able to predict the experimental results. Thus it is conclude that the binding of these ions to hemoglobin is a cooperative phenomenon. (Author) [pt

Fasting serum glucose and glycosylated hemoglobin level in obesity.

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Das, R K; Nessa, A; Hossain, M A; Siddiqui, N I; Hussain, M A

2014-04-01

Obesity is a condition in which the body fat stores are increased to an extent which impairs health and leads to serious health consequences. The amount of body fat is difficult to measure directly, and is usually determined from an indirect measure - the body mass index (BMI). Increased BMI in obese persons is directly associated with an increase in metabolic disease, such as type 2 diabetes mellitus. This Analytical cross sectional study was undertaken to assess the relation between obesity and glycemic control of body by measuring fasting serum glucose and glycosylated hemoglobin. This study was carried out in the Department of Physiology, Mymensingh Medical College, Mymensingh from 1st July 2011 to 30th June 2012 on 120 equally divided male and female persons within the age range of 25 to 55 years. Age more than 55 years and less than 25 years and diagnosed case of Hypothyroidism, Cushing's syndrome, polycystic ovary, Antipsychotic drug user and regular steroid users were excluded. Non probability purposive type of sampling technique was used for selecting the study subjects. Measurement of body mass index was done as per procedure. Fasting serum glucose was estimated by glucose oxidase method and Glycosylated hemoglobin by Boronate Affinity method. Statistical analysis was done by SPSS (version 17.0). Data were expressed as Mean±SE and statistical significance of difference among the groups were calculated by unpaired student's 't' test and Pearson's correlation coefficient tests were done as applicable. The Mean±SE of fasting serum glucose was significant at 1% level (P value obese group of BMI. There was no significant difference of glycosylated hemoglobin level between control and study groups. But there was positive correlation within each group. Fasting serum glucose also showed a bit stronger positive correlation with BMI. Both obese male and female persons showed higher levels of fasting serum glucose and glycosylated hemoglobin. The observed positive

Hemoglobin drop after anesthesia in craniosynstosis: Dilemma of operate or not to operate

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El-Ghandour, Nihal; Kassem, Salah; Al Sabbagh, Abdelrahman J.; Al-Banyan, Ayman; Shubbak, Firas A.; Hassib, Ahmad; Zaki, Hazem

2011-01-01

An infant with craniosynostosis for craniectomy and cranial-vault remodelling was detected to have very low hemoglobin (6.8 gm%) after induction of anesthesia. This posed a dilemma whether to proceed with or abandon the surgical procedure. The case was postponed and was rescheduled for surgery one week later with hope that his hemoglobin would rise during this period. However, even before second anesthesia his hemoglobin level was found to be unchanged. Meticulous anesthesia management resulted in uneventful surgical procedure. PMID:25885398

City’s lost jewel. History of Cracow’a All Saints church till the end of XVI century

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Katarzyna Walczak

2013-12-01

Full Text Available One of the oldest churches in Krakow, dedicated to All Saints, was situated between Grodzka and Franciszkańska Street. It was build in the XIII century, could had been replacement church for first Cracow’s parish, which was transferred from Saint Trinity church. The name of All Saints church was mentioned for the first time in Vita S. Stanislai. The second time in 1278 his rector Arnold was noted. Most interesting fact is that in the Tables of Pence from 1325–1327 you can find two rectors, who had separate salary and were called rectors of wooden and brick churches. In 1490 church got a collegiate title. Than double rectory was liquidated and positions of provost and dean were established. Church was a place where Brotherhood of the Poor created by Bishop Zbigniew Oleśnicki used to work. Our knowledge of churches looks comes from iconography which was made when the building was already a ruin. According to those sources church had two naves, was oriented and had simply completed presbytery. The building had adhered tower, sacristy, treasure and chapel, called capella antiqua. Description of the interior of the church comes from 1599, when Jerzy Radziwiłł, Bishop of Cracow had visited it. According to the decision of the City Beautification Committee, the church was demolished in 1835, and church’s equipment was sold on auction. The tower survived till 1840, when it shared the fate of the rest of the building.

Utility of noninvasive transcutaneous measurement of postoperative hemoglobin in total joint arthroplasty patients.

Science.gov (United States)

Stoesz, Michael; Wood, Kristin; Clark, Wesley; Kwon, Young-Min; Freiberg, Andrew A

2014-11-01

This study prospectively evaluated the clinical utility of a noninvasive transcutaneous device for postoperative hemoglobin measurement in 100 total hip and knee arthroplasty patients. A protocol to measure hemoglobin noninvasively, prior to venipuncture, successfully avoided venipuncture in 73% of patients. In the remaining 27 patients, there were a total of 48 venipunctures performed during the postoperative hospitalization period due to reasons including transcutaneous hemoglobin measurement less than or equal to 9 g/dL (19), inability to obtain a transcutaneous hemoglobin measurement (8), clinical signs of anemia (3), and noncompliance with the study protocol (18). Such screening protocols may provide a convenient and cost-effective alternative to routine venipuncture for identifying patients at risk for blood transfusion after elective joint arthroplasty. Copyright © 2014 Elsevier Inc. All rights reserved.

Effect of water and ethanol radicals on the protein part of human hemoglobin

International Nuclear Information System (INIS)

Szweda-Lewandowska, Z.; Puchala, M.

1989-01-01

This paper studies the changes in the tryptophan fluorescence in human hemoglobin induced by ·OH, e aq - , H atoms and ethanol radicals. Measurements of irradiated hemoglobin performed in phosphate buffer, pH 7, indicate that the processes of unfolding a protein are induced with the highest efficiency by the ·OH radicals. A destructive action of e aq - is more evident in the absence of the ·OH radicals. Fluorescence measurements carried out after incubation of irradiated hemoglobin in Gdn·HCl solution reveal the tryptophan residues destruction, which is relatively small (at 2.5 kGy maximum fluorescence decrease was about 23%) and caused by the ·OH radicals. Within the dose range, the participation of the e aq - , H atoms and ethanol radicals in hemoglobin tryptophan residue destruction can be neglected. (author)

Diode laser irradiation of rat blood and its effect on hemoglobin and plasma

International Nuclear Information System (INIS)

Saad-El-Din, A.A.; El-Ahdaal, M.A.; Omran, M.F.

2002-01-01

Blood was exposed to diode laser irradiation of wavelength 830 nm and maximum powe of 31.4 MW, with exposure times 15, 30, 45 and 60 minutes. Hemoglobin IR spectra and X-ray crystallography, plasma Na + , K + , Ca + +. cholesterol concentrations and viscosity were measured. There were changes in hemoglobin amide groups as well as changes in the X-ray in hemoglobin structure. Decreases in both Na concentration and plasma viscosity occurred at 15 and 30 minutes of laser exposure. On increasing time to 45 and 60 minutes, the Na concentration and viscosity were increased. K, Ca and cholesterol concentration were decreased linearly with time. Na / K ratio was increased also with time of exposure. The results have been indicated that the diode laser affect the secondary structure of hemoglobin, membranes structures and plasma

Pretreatment hemoglobin in early stage glottic cancer: red flag or red herring?

International Nuclear Information System (INIS)

Tucker, J. Curtis; Hixson, William C.; Kim, Robert Y.; Smith, Judy W.; Mayo, Matthew S.

1996-01-01

Purpose: To evaluate the impact of pretreatment hemoglobin levels on the recurrence rate of patients with early stage glottic carcinoma treated with definitive radiotherapy. Materials and Methods: Between May 1972 and December 1992, one hundred three patients with stage I or II glottic carcinoma were treated with definitive radiotherapy. The records were reviewed and analyzed for the effects of pretreatment hemoglobin and hematocrit, stage, dose per fraction (180 vs 200 cGy), treatment interruptions, and differentiation of the tumor. The majority of stage I patients were treated to a total dose of 64 to 66 Gy, and stage II patients were treated to 68 to 70 Gy. Mean and median follow up were 85 and 75 months, respectively. Results: With mean follow up of 85 months, the recurrence rate for patients with hemoglobin ≤13 g/dL was 33.3%, and patients with hemoglobin >13 g/dL was 20% (p 0.19). For stage I (n = 84), the recurrence rate with hemoglobin ≤13 g/dL and >13 g/dL was 26% and 17%, respectively (p = 0.51). For stage II patients (n = 19) with hemoglobin ≤13 g/dL and >13 g/dL, the recurrence rate was 50% and 36%, respectively (p = 0.66). Using a Fisher's exact test, the only variables showing a statistically significant prognosis for recurrence were treatment interruption and stage. The recurrence rate with a treatment interruption was 40% compared with 15% if there was no interruption (p 0.0039). The recurrence rate for stage I was 19%, and for stage II was 42% (p = 0.067). Other factors showed no significant increase in recurrence; dose per fraction (p = 0.421), grade of differentiation (p = 0.740). Conclusion: While pretreatment hemoglobin levels below 13 g/dL do not significantly affect the recurrence rates for T1N0 and T2N0 glottic carcinomas, treatment interruptions are a significant factor in the failure of these patients

TECHNIQUES OF EVALUATION OF HEMOGLOBIN OXYGEN SATURATION IN CLINICAL OPHTHALMOLOGY

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S. Yu. Petrov

2016-01-01

Full Text Available Oxygen content in body fluids and tissues is an important indicator of life support functions. A number of ocular pathologies, e.g. glaucoma, are of presumable vascular origin which means altered blood supply and oxygen circulation. Most oxygen is transported in the blood in the association with hemoglobin. When passing through the capillaries, hemoglobin releases oxygen, converting from oxygenated form to deoxygenated form. This process is accompanied by the changes in spectral characteristics of hemoglobin which result in different colors of arterial and venous blood. Photometric technique for the measurement of oxygen saturation in blood is based on the differences in light absorption by different forms of hemoglobin. The measurement of saturation is called oximetry. Pulse oximetry with assessment of tissue oxygenation is the most commonly used method in medicine. The degree of hemoglobin oxygen saturation in the eye blood vessels is the most accessible for noninvasive studies during ophthalmoscopy and informative. Numerous studies showed the importance of this parameter for the diagnosis of retinopathy of various genesis, metabolic status analysis in hyperglycemia, diagnosis and control of treatment of glaucoma and other diseases involving alterations in eye blood supply. The specific method for evaluation of oxygen concentration is the measurement of pressure of oxygen dissolved in the blood, i.e. partial pressure of oxygen. In ophthalmological practice, this parameter is measured in anterior chamber fluid evaluating oxygen level for several ophthalmopathies including different forms of glaucoma, for instillations of hypotensive eye drops as well as in vitreous body near to the optic disc under various levels of intraocular pressure. Currently, monitoring of oxygen saturation in retinal blood vessels, i.e. retinal oximetry, is well developed. This technique is based on the assessment of light absorption by blood depending on

Carbon monoxide reduces near-infrared spectroscopy determined 'total' hemoglobin

DEFF Research Database (Denmark)

Niemann, Mads J; Sørensen, Henrik; Siebenmann, Christoph

2017-01-01

with CO (1.5 mL kg-1) was added to the circuit. Two NIRS systems (NIRO-200NX and INVOS-5100) assessed ScO2 as the ratio of oxygenated to deoxygenated hemoglobin, while venous blood samples were analyzed for carboxyhemoglobin (COHb). After CO/O2 rebreathing COHb increased to 8.7% (IQR; 7.9-9.4; p = .004...... to normoxia (68.9 ± 6.9%; p hemoglobin decreased (by 19.7 μM (median; IQR 2.8-34.8; p = .016) and 37.3 μM (30.8-46.6; p = .004), respectively) during inhalation of CO/O2 compared...... to inhalation of O2. Therefore, NIRO-200NX determined 'total' hemoglobin (sum of O2Hb and HHb) decreased (by 62.1 μM; 44.5-78.2; p = .001). In conclusion, exposure to CO did not increase MCAVmean, and neither NIRO-200NX nor INVOS-5100 detected a change in ScO2 when CO was added to inhalation of oxygen...

Pitfalls in the biological diagnosis of common hemoglobin disorders.

Science.gov (United States)

Wajcman, Henri; Moradkhani, Kamran

2015-01-01

In West-European countries, hemoglobin disorders are no more rare diseases. Programs for diagnosis of heterozygous carriers have been established to prevent cases with major sickle cell disease or thalassemias. These studies have been done essentially by high performance liquid chromatography on cation-exchange columns and electrophoresis (mostly capillary electrophoresis). They have been done through systematic population studies or premarital diagnosis. We describe in this work the frequent or rare pitfalls encountered, which led to false negative or positive diagnosis both in the field of sickle cell disease and thalassemias. In the absence of a well identified hemoglobin disorder in the proband's family, it is a rule that the use of a single test is insufficient to identify formally HbS. The presence of HbS could also be masked by another hemoglobin abnormality. The sole measurement of HbA2 level is insufficient to characterize a thalassemic trait: this level needs always to be interpreted considering RBC parameters and iron metabolic status. In difficult cases, the definitive answer may require a family study and/or a molecular genetic characterization.

Hypoxic survival requires a 2-on-2 hemoglobin in a process involving nitric oxide

Science.gov (United States)

Hemschemeier, Anja; Düner, Melis; Casero, David; Merchant, Sabeeha S.; Winkler, Martin; Happe, Thomas

2013-01-01

Hemoglobins are recognized today as a diverse family of proteins present in all kingdoms of life and performing multiple reactions beyond O2 chemistry. The physiological roles of most hemoglobins remain elusive. Here, we show that a 2-on-2 (“truncated”) hemoglobin, termed THB8, is required for hypoxic growth and the expression of anaerobic genes in Chlamydomonas reinhardtii. THB8 is 1 of 12 2-on-2 hemoglobins in this species. It belongs to a subclass within the 2-on-2 hemoglobin class I family whose members feature a remarkable variety of domain arrangements and lengths. Posttranscriptional silencing of the THB8 gene results in the mis-regulation of several genes and a growth defect under hypoxic conditions. The latter is intensified in the presence of an NO scavenger, which also impairs growth of wild-type cells. As recombinant THB8 furthermore reacts with NO, the results of this study indicate that THB8 is part of an NO-dependent signaling pathway. PMID:23754374

Identification of hemoglobin variants by top-down mass spectrometry using selected diagnostic product ions.

Science.gov (United States)

Coelho Graça, Didia; Hartmer, Ralf; Jabs, Wolfgang; Beris, Photis; Clerici, Lorella; Stoermer, Carsten; Samii, Kaveh; Hochstrasser, Denis; Tsybin, Yury O; Scherl, Alexander; Lescuyer, Pierre

2015-04-01

Hemoglobin disorder diagnosis is a complex procedure combining several analytical steps. Due to the lack of specificity of the currently used protein analysis methods, the identification of uncommon hemoglobin variants (proteoforms) can become a hard task to accomplish. The aim of this work was to develop a mass spectrometry-based approach to quickly identify mutated protein sequences within globin chain variants. To reach this goal, a top-down electron transfer dissociation mass spectrometry method was developed for hemoglobin β chain analysis. A diagnostic product ion list was established with a color code strategy allowing to quickly and specifically localize a mutation in the hemoglobin β chain sequence. The method was applied to the analysis of rare hemoglobin β chain variants and an (A)γ-β fusion protein. The results showed that the developed data analysis process allows fast and reliable interpretation of top-down electron transfer dissociation mass spectra by nonexpert users in the clinical area.

An Atomistic View on Human Hemoglobin Carbon Monoxide Migration Processes

Science.gov (United States)

Lucas, M. Fátima; Guallar, Víctor

2012-01-01

A significant amount of work has been devoted to obtaining a detailed atomistic knowledge of the human hemoglobin mechanism. Despite this impressive research, to date, the ligand diffusion processes remain unclear and controversial. Using recently developed computational techniques, PELE, we are capable of addressing the ligand migration processes. First, the methodology was tested on myoglobin's CO migration, and the results were compared with the wealth of theoretical and experimental studies. Then, we explored both hemoglobin tense and relaxed states and identified the differences between the α-and β-subunits. Our results indicate that the proximal site, equivalent to the Xe1 cavity in myoglobin, is never visited. Furthermore, strategically positioned residues alter the diffusion processes within hemoglobin's subunits and suggest that multiple pathways exist, especially diversified in the α-globins. A significant dependency of the ligand dynamics on the tertiary structure is also observed. PMID:22385860

Prevalence of overweight, obesity and underweight among 5-year-old children in Saint Lucia by three methods of classification and a comparison with historical rates.

Science.gov (United States)

Gardner, K; Bird, J; Canning, P M; Frizzell, L M; Smith, L M

2011-01-01

The study aimed to determine if child obesity rates have risen in the Caribbean nation of Saint Lucia, as found globally, and whether under-nutrition coexists, as in other developing nations. The average adult in Saint Lucia is overweight, thus considerable child obesity might be expected, but there are no current data. Heights and weights were obtained from a sample (n= 425) of the 2001 birth cohort of Saint Lucian children measured during the nation-wide 2006/2007 Prior to School Entry Five-Year Assessment. Prevalence of overweight, obesity and underweight were estimated by Centers for Disease Control (CDC), Cole et al. and new World Health Organization (WHO) methods. Previously reported 1976 estimates, including children ≤60 months of age only, based on National Centre for Health Statistics curves, were adjusted to new WHO equivalents using an algorithm developed by Yang and de Onis, and compared with rates in our subsample of children ≤60 months of age (n= 99). Regardless of classification method, overweight and obesity rates were high: 14.4% and 9.2% (WHO); 11.3% and 12.0% (CDC); and 9.9% and 7.1% (Cole et al.), respectively. Underweight estimates also varied: 4.7% (WHO); 11.3% (CDC) and 6.6% (Cole et al.). Obesity in our young subsample (15.2%; WHO) was more than 3 times the adjusted 1976 rate (4.3%). Obesity among Saint Lucian pre-schoolers has tripled in 30 years. Our findings also suggest that this country, like many undergoing a 'nutrition transition', faces the dual challenge of over-nutrition and under-nutrition. Routine monitoring of overweight and underweight is needed in Saint Lucia, as is the implementation and evaluation of programmes to address these problems. © 2010 Blackwell Publishing Ltd.

Assessing the relative stabilities of engineered hemoglobins using electrospray mass spectrometry.

Science.gov (United States)

Apostol, I

1999-07-15

An ion trap mass spectrometer equipped with an electrospray source was used to examine the relative thermodynamic stabilities of various hemoglobins with respect to both tetramer dissociation and hemin dissociation. The results demonstrated that the stability of hemoglobin molecules can be differentiated by the amount of applied collision-induced dissociation (CID) energy necessary to break up the intact tetramer into its constituent globins. The stability of the intact tetramer was affected by single mutations in the beta-globins. The stabilities of the constituent hologlobins were assessed via trap CID of selected ions. The results demonstrated the importance of the contributions of the hologlobin components to the stability of the intact tetramer. Genetic fusion of two alpha-globins, through the introduction of a single glycine residue between the C-terminus of one alpha-chain and the N-terminus of the second, significantly increased the stability of the hemoglobin pseudo-tetramer. Chemical crosslinking of the beta-globins in addition to genetic fusion of alpha-globins further stabilized the hemoglobin molecule. A dihemoglobin molecule produced by the genetic fusion of two di-alpha-globins with a flexible linker demonstrated a decreased stability relative to the corresponding monohemoglobin. Copyright 1999 Academic Press.

High-altitude adaptations in vertebrate hemoglobins

DEFF Research Database (Denmark)

Weber, Roy E.

2007-01-01

Vertebrates at high altitude are subjected to hypoxic conditions that challenge aerobic metabolism. O2 transport from the respiratory surfaces to tissues requires matching between the O2 loading and unloading tensions and theO2-affinity of blood, which is an integrated function of hemoglobin......, birds and ectothermic vertebrates at high altitude....

Electronic structure of human hemoglobin: ultrasoft X-ray emission study

International Nuclear Information System (INIS)

Soldatov, A.V.; Kravtsova, A.N.; Fedorovich, E.N.; Kurmaev, E.Z.; Moewes, A.

2004-01-01

Full text: The iron L 2,3 and carbon, nitrogen and oxygen Kα X-ray emission spectra (XES) of human hemoglobin have been recorded at the soft X-ray spectroscopy endstation on Undulator Beam line 8.0 at Advanced Light Source (ALS) located at the Lawrence Berkeley National Laboratory. The theoretical calculations of Fe L 3 -XES have been performed using ab initio code FEFF8.2. The calculations have been carried out for the structure of hemoglobin presented in PDB (entry 3HHB) as well as for the molecule with symmetrical heme plane. It was found that the Fe L 3 emission spectrum calculated for the ideal molecule agrees slightly better with the experiment as compared with those calculated for the real molecule. Thus, one can use the structure of the ideal molecule for theoretical Fe L 3 -XES simulations. The theoretical analysis has shown that the fist peak of experimental Fe L 3 - emission spectrum is enhanced by the nearest nitrogen atoms lying in heme plane around the central iron atom. The theoretical C K- and N K-XES spectra of hemoglobin have been calculated. A good agreement between theoretical and experimental spectra has been obtained. The distribution of the partial electronic densities of states in the valence and conduction bands of hemoglobin has been determined

Effects of Protection and Sediment Stress on Coral Reefs in Saint Lucia.

Science.gov (United States)

Bégin, Chantale; Schelten, Christiane K; Nugues, Maggy M; Hawkins, Julie; Roberts, Callum; Côté, Isabelle M

2016-01-01

The extent to which Marine Protected Areas (MPAs) benefit corals is contentious. On one hand, MPAs could enhance coral growth and survival through increases in herbivory within their borders; on the other, they are unlikely to prevent disturbances, such as terrestrial runoff, that originate outside their boundaries. We examined the effect of spatial protection and terrestrial sediment on the benthic composition of coral reefs in Saint Lucia. In 2011 (10 to 16 years after MPAs were created), we resurveyed 21 reefs that had been surveyed in 2001 and analyzed current benthic assemblages as well as changes in benthic cover over that decade in relation to protection status, terrestrial sediment influence (measured as the proportion of terrigenous material in reef-associated sediment) and depth. The cover of all benthic biotic components has changed significantly over the decade, including a decline in coral and increase in macroalgae. Protection status was not a significant predictor of either current benthic composition or changes in composition, but current cover and change in cover of several components were related to terrigenous content of sediment deposited recently. Sites with a higher proportion of terrigenous sediment had lower current coral cover, higher macroalgal cover and greater coral declines. Our results suggest that terrestrial sediment is an important factor in the recent degradation of coral reefs in Saint Lucia and that the current MPA network should be complemented by measures to reduce runoff from land.

Effects of Protection and Sediment Stress on Coral Reefs in Saint Lucia.

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Chantale Bégin

Full Text Available The extent to which Marine Protected Areas (MPAs benefit corals is contentious. On one hand, MPAs could enhance coral growth and survival through increases in herbivory within their borders; on the other, they are unlikely to prevent disturbances, such as terrestrial runoff, that originate outside their boundaries. We examined the effect of spatial protection and terrestrial sediment on the benthic composition of coral reefs in Saint Lucia. In 2011 (10 to 16 years after MPAs were created, we resurveyed 21 reefs that had been surveyed in 2001 and analyzed current benthic assemblages as well as changes in benthic cover over that decade in relation to protection status, terrestrial sediment influence (measured as the proportion of terrigenous material in reef-associated sediment and depth. The cover of all benthic biotic components has changed significantly over the decade, including a decline in coral and increase in macroalgae. Protection status was not a significant predictor of either current benthic composition or changes in composition, but current cover and change in cover of several components were related to terrigenous content of sediment deposited recently. Sites with a higher proportion of terrigenous sediment had lower current coral cover, higher macroalgal cover and greater coral declines. Our results suggest that terrestrial sediment is an important factor in the recent degradation of coral reefs in Saint Lucia and that the current MPA network should be complemented by measures to reduce runoff from land.

Dichloromethane as an antisickling agent in sickle cell hemoglobin

Energy Technology Data Exchange (ETDEWEB)

Schoenborn, B.P.; North, B.E.

1977-01-01

Observations are reported that show that dichloromethane (DCM) does have a significant effect on the oxygen binding properties of hemoglobin. At DCM pressures high enough to prevent or reverse sickling, DCM would lower the oxygen affinity of hemoglobin, therefore reducing oxygen transport at low oxygen pressure. This decrease in oxygen affinity might, however, increase the oxygen availability to tissue as long as a sufficiently large lung P/sub O/sub 2// is maintained. Crystallographic studies show that site D4 has a much lower affinity for DCM than site D3 while sites D1 and D2 show a higher affinity.

Non equivalence of the chains in the allosteric interaction of the hemoglobin

International Nuclear Information System (INIS)

Jacchieri, S.G.

1983-01-01

The importance, for the temperature dependence of the cooperative behaviour of hemoglobin, of the functional non equivalence of the polypeptide chains from which the hemoglobin molecule is built is studied. With such purpose thermodynamic allosteric parameters are introduced called 'mean allosteric parameters' which relate the last two oxygen bindings to the firsttwo ones. It is shown that the mean allosteric free energy is strongly correlated to the Hill parameter which is a classic measure of cooperativity; hence, the mean allosteric free energy measures the hemoglobin cooperativity. Recent experimental data show that the mean allosteric free energy decreasses with temperature; this is due to the mean allosteric enthalphy and entropy being positive quantities. To analise such behaviour in terms of thermodynamic's arguments equations are derived for the thermodynamic parameters of oxygen binding to hemoglobin in terms of those of its chains. Since the obtained equations have a great number of terms the same treatment is applied to a hypothetic dimer from which simpler relations are derived. From both cases it is concluded that the positive character of the mean allosteric enthalpy and entropy is due to the presence of cooperative and anticooperative terms. Since the last terms are absent in the equations of allosteric homoproteins, the characteristic temperature-dependence of hemoglobin's cooperativity depends on the presence of non-equivalent chains. (Author) [pt

Patterns of glycemic control using glycosylated hemoglobin in diabetics

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Arunpreet Singh Kahlon

2011-01-01

Full Text Available Aim : Till now estimation of blood glucose is the highly effective method for diagnosing diabetes mellitus but it provides a short-term picture of control. More evidence is required to prove that plasma glucose and glycosylated hemoglobin levels together gives a better estimate of glycemic control and compliance with treatment. Indian diabetes risk score (IDRS is a simplified screening tool for identifying undiagnosed diabetic subjects, requires minimum time, and effort and can help to considerably reduce the costs of screening. Objective : To study patterns of glycemic control using glycosylated hemoglobin in diabetic patients. To find out correlation between levels of plasma glucose and glycosylated hemoglobin in diabetics and to calculate IDRS of the study population. Materials and Methods : A cross sectional study was conducted among 300 known diabetic patients attending outpatient department of a rural medical college in Haryana, India. Following standard procedures and protocols FPG and glycosylated hemoglobin were measured to find out a pattern of glycemic control in them after taking their written and informed consent. A correlation between the levels of glycosylated hemoglobin and fasting blood glucose was also calculated. These patients were made to fill a performa and their demographic and clinical risk factors were noted and based on this, their IDRS was calculated. This was done to validate the IDRS in Indian rural population. Results : Fifty-two percent of the population had fasting plasma glucose level between 125-150 mg/dl, 21% had this level between 151-175 mg/dl. Thirteen percent of the study subjects had HbA1C between 6.5-7.5, more than half (57.3% had this value between 7.5-8.5, 12% and 18% had values between 8.5-9.5 and 9.5-10.5, respectively. Twelve percent of the participants had HbA1C level higher than 10.5. Correlation of fasting plasma glucose level and HbA1C was also studied and found that correlation coefficient came

The Cerrado of Saint-Hilaire: naturalists representations of a 19th century French traveler

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Lorena Borges Silva

2016-12-01

Full Text Available This work aims to investigate the different motivations and goals behind the traveler Saint-Hilarie intentions to invest in a project to enter the Cerrado, and from his narratives and perceptions, we intend to understand how the nature of the Cerrado was interpreted during the nineteenth century. We seek to see why so many derogatory assertions were made by observing the natural surroundings of Goiás.

Methylation of hemoglobin to enhance flocculant performance

Science.gov (United States)

An inexpensive bioflocculant, bovine hemoglobin (Hb), has been covalently modified through methylation of the side chain carboxyl groups of aspartic and glutamic acid residues to improve its flocculation activity. Potentiometric titration of the recovered products showed approximately 28% degree of ...

Iron bioavailability of maize hemoglobin in a Caco-2 cell culture model

Science.gov (United States)

Maize is an important staple crop in many parts of the world but has low iron bioavailability, in part due to its high phytate content. Hemoglobin is a form of iron that is highly bioavailable and its bioavailability is not inhibited by phytate. We hypothesize that maize hemoglobin is a highly bioav...

Santa Rosa de Lima: uma santa anoréxica na América Latina? Santa Rosa de Lima: ¿una santa anoréxica en Latinoamérica? Saint Rose of Lima: an anorexic saint in Latin America?

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Cybelle Weinberg

2005-04-01

Full Text Available A literatura especializada tem nos mostrado que é grande a semelhança entre o comportamento alimentar das santas jejuadoras medievais e o das anoréxicas atuais. No entanto, há poucas referências sobre a existência de santas jejuadoras na América Latina. No presente trabalho, os autores salientam aspectos da vida de Santa Rosa de Lima, Patrona da América e Filipinas, que poderiam ser descritos como comportamentos anoréxicos. Comparando os aspectos psicopatológicos da vida de Santa Rosa com o comportamento das anoréxicas atuais, pareceu confirmar-se um padrão uniforme de comportamento, especialmente quanto ao aspecto imitativo, fator que poderia levar as jovens da atualidade a desenvolverem um transtorno alimentar após terem algum tipo de informação sobre o transtorno.La literatura especializada nos muestra gran similitud entre el comportamiento alimentar de las santas de la edad media que ayunaban y el de las anoréxicas de la actualidad. Hay pocas informaciones sobre santas que practicaban ayuno en Latinoamérica. En este artículo, los autores resaltan aspectos de la vida de santa Rosa de Lima, Patrona de América y Filipinas, que pueden ser considerados como comportamientos anoréxicos. Al comparar aspectos psicopatológicos de la vida de santa Rosa con el comportamiento de anoréxicas de la actualidad, parece que se confirma un modelo uniforme de comportamiento, especialmente respecto al aspecto de imitación, hecho que podría llevar a las jóvenes de hoy a desarrollar un trastorno de la alimentación luego de recibir informaciones sobre el tema.The specialized literature has shown us the great similarity between the eating habits of medieval fasting women saints and today's anorexic women. Nonetheless, there are few references to the existence of such fasting women saints in Latin America. The authors of this paper present some aspects of the life of Saint Rose of Lima, the patroness of the Americas and the Philippines, that

Une étude de cas. Saint Sébastien dans “Je suis sang” de Jan Fabre

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Lydie Toran

2017-06-01

Full Text Available Jan Fabre’s writing frequently displays a variety of quotations, especially visual ones. His 2001 Je suis sang (conte de fées médiéval is directly and indirectly inspired by Saint Sebastian’s iconography. This study examines the modalities and functions of this re-use in detail.

Analysis of the binding interaction in uric acid - Human hemoglobin system by spectroscopic techniques

Science.gov (United States)

Makarska-Bialokoz, Magdalena

2017-05-01

The binding interaction between human hemoglobin and uric acid has been studied for the first time, by UV-vis absorption and steady-state, synchronous and three-dimensional fluorescence techniques. Characteristic effects observed for human hemoglobin intrinsic fluorescence during interaction with uric acid at neutral pH point at the formation of stacking non-covalent and non-fluorescent complexes. All the calculated parameters, the binding, fluorescence quenching and bimolecular quenching rate constants, as well as Förster resonance energy transfer parameters confirm the existence of static quenching. The results of synchronous fluorescence measurements indicate that the fluorescence quenching of human hemoglobin originates both from Trp and Tyr residues and that the addition of uric acid could significantly hinder the physiological functions of human hemoglobin.

21 CFR 866.5470 - Hemoglobin immunological test system.

Science.gov (United States)

2010-04-01

.... Measurements of free hemoglobin aid in the diagnosis of various hematologic disorders, such as sickle cell... blood cells), and leukemia (cancer of the blood-forming organs). (b) Classification. Class II...

«O Sancta Haera». Pilgrims and Pilgrimage in the Sanctuary of Sainte Foy at Conques

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Marco Papasidero

2017-12-01

Full Text Available The Liber miraculorum Sancte Fidis, written during the XI century, partially by Bernard of Angers, describes miracles, healings and rituals practiced in the sanctuary of Sainte Foy at Conques, martyr of the beginning of the IV century.The Liber allows us to analyze the anthropological and religious dimension of the pilgrims and their pilgrimage. As a matter of fact, through the descriptions of the pilgrims, their gestures, their aspects, their words, their ritual practices by the tomb, their rich or poor offerings, we can analyze the dimension of the Medieval pilgrimage as a specific case study, which can help us describe the idea of pilgrimage in Medieval West Europe and specifically in this sanctuary.Furthermore, through the reading of the accounts of miracle stories, we can analyze the healing ritual practices, the miraculous dimension of this cult and the Sainte’s ability to treat diseases. The center of the Conques cult was – and remains – the Majesty of Sainte Foy, a reliquary statue in which the power (virtus/dynamis of the saint’s relics is concentrated.

Constraints on mutational pathways of hemoglobin evolution

DEFF Research Database (Denmark)

Kumar, Amit; Natarajan, Chandrasekhar; Moriyama, Hideaki

2016-01-01

amino acid substitutions that occurred during an evolutionary reduction in hemoglobin (Hb)-O2 affinity in nightjars (nocturnal birds in the family Caprimulgidae).We selected nightjar Hbs for experimental study because ancestral sequence reconstructions indicated that the evolved reduction in Hb-O2...

Scyllarides obtusus spec. nov., the scyllarid lobster of Saint Helena, Central South Atlantic (Crustacea: Decapoda Reptantia: Scyllaridae)

NARCIS (Netherlands)

Holthuis, L.B.

1993-01-01

A new species of slipper lobster is described from Saint Helena, where the species seems to be endemic. Known under the vernacular name Stump, the species forms the subject of a local fishery, carried out since early times. Previously the species has been identified with various Scyllarides species

Hemoglobin E in Northeast India: A review on its origin, distribution, migration and health implication

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Sikdar Mithun

2016-09-01

Full Text Available A systematic review of the studies on hemoglobin E in Northeast India has been carried out to understand the magnitude of research undertaken on this aspect during the last seven decades. Owing to the high prevalence of hemoglobin E in this part of India different authors have studied this hemoglobin from different perspectives and found conflicting results. However a systematic review of such studies is lacking from a holistic point of view. Most of the epidemiological, in vitro as well as in vivo studies show signatures of selection with this hemoglobin locus. However, how this polymorphism is maintained at different rates at different geographical region is still a matter of contention. This review will fill the gap from all perspectives starting from the frequency distribution of hemoglobin E and its spread in different parts of Northeast India, its relationship with malaria hypothesis, the population migration, population affinity and most importantly the health implication arising out of it. A probable origin of hemoglobin E among an Austroasiatic population of Northeast India has been postulated with the help of advance molecular anthropological knowledge like the deep rooted markers of mt DNA and Y-chromosome haplotypes.

Substitutions in woolly mammoth hemoglobin confer biochemical properties adaptive for cold tolerance

DEFF Research Database (Denmark)

Campbell, Kevin L.; Roberts, Jason E.E.; Watson, Laura N.

2010-01-01

We have genetically retrieved, resurrected and performed detailed structure-function analyses on authentic woolly mammoth hemoglobin to reveal for the first time both the evolutionary origins and the structural underpinnings of a key adaptive physiochemical trait in an extinct species. Hemoglobin...... binds and carries O2; however, its ability to offload O2 to respiring cells is hampered at low temperatures, as heme deoxygenation is inherently endothermic (that is, hemoglobin-O2 affinity increases as temperature decreases). We identify amino acid substitutions with large phenotypic effect...... the Pleistocene period. This powerful new approach to directly analyze the genetic and structural basis of physiological adaptations in an extinct species adds an important new dimension to the study of natural selection....

Educação integral: notas sobre Charles Fourier, Saint Simon e Pierre-Joseph Proudhon

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José Damiro Moraes

2015-11-01

Full Text Available Este artigo procura contribuir para as discussões em torno do conceito histórico de educação integral, tendo em vista que existem algumas lacunas em torno desse tema. Aqui iremos abordar os conceitos de educação integral no pensamento dos intelectuais: Claude-Henri de Rouvroy ou Conde de Saint-Simon (1760-1825, François Marie Charles Fourier (1772-1837 e Pierre-Joseph Proudhon (1809-1865. A pesquisa utiliza as obras dos autores escritas/publicadas no século XIX. A metodologia procura entender a produção intelectual de maneira dialética e dentro do contexto histórico em que foi produzida. Indicamos como conclusões que Proudhon, Saint-Simon e Charles Fourier, consideravam no processo educacional que a parte literária estaria diretamente vinculada com a científica, unindo teoria e prática. Podemos aventar que as contribuições desses pensadores foram importante nas reflexões educacionais e na defesa de uma educação comprometida com a transformação social presente no pensamento socialista e no movimento operário do século XIX e XX.

Why are there two kinds of chain in tetrameric hemoglobins

International Nuclear Information System (INIS)

Ferreira, R.; Jacchieri, S.G.

1981-01-01

The homeotropic allosteric interactions responsible for the sigmoidal oxygen saturation curves of α 2 β 2 hemoglobins are shown to be larger than those of hypothetical hemoglobins obeying identical curves and built from equivalent chains, γ 4 . It is also shown that this ensures for the α 2 β 2 species a more dependable cooperativity, through a biologically significative temperature range. On the basis of these findings it is argued that the existence of two different globin chains is advantageous in an evolutionary sense. (Author) [pt

Urban growth and cultural identity; fractures and imbalances in heritage values: A case study of the island of Saint-Louis, Senegal

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Lucía Martínez-Quintana

2016-05-01

Full Text Available The island of Saint-Louis of Senegal was awarded the status of world heritage site by UNESCO in 2000 as an “outstanding example” of urban heritage. This island city comes with a unique heritage: development planning that combines a strong historical French influence with a gridiron urban morphology and building typology. The island must be interpreted within its total territorial context that includes both the island of Sor (on the mainland and La Langue de la Barberie, a sandy barrier that separates the mouth of the river from the sea. The city of Saint-Louis itself has grown enormously and haphazardly from the latter part of the 20th century: it is now the fourth most populous city in Senegal. At present, the city is undergoing a serious period of decline and recession due, in part, to the overriding influence of the capital, Dakar, and the centralized political forces in the country. This article looks at the key morphological and functional reasons behind the development and evolution of the island of Saint-Louis and that persist in the present context, with justifications for the deep-rooted heritage values that maintain its prestige as a World Heritage Site.

Hemoglobin Function in Stored Blood.

Science.gov (United States)

1974-08-01

States during 1973. Several advantages over ACA) are important. Blood stored in CPD maintains higher ./ levels of 2,3-DPG (2,3- diphosphoglycerate ) and a...survival and ATP levels in stored blood is explained by the several functions of ATP which are necessary for cell viability. However, ATP levels do...not correlate with oxygen affinity during storage. Levels of 2,3-DPG determine oxygen affinity and thus hemoglobin function. (12,13) When normal levels

In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure.

Science.gov (United States)

Wang, Yu; Hu, Song; Maslov, Konstantin; Zhang, Yu; Xia, Younan; Wang, Lihong V

2011-04-01

We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to noninvasively image hemoglobin oxygen saturation (sO₂) and oxygen partial pressure (pO₂) in vivo in single blood vessels with high spatial resolution. While PAM measures sO₂ by imaging hemoglobin optical absorption at two wavelengths, FCM quantifies pO₂ using phosphorescence quenching. The variations of sO₂ and pO₂ values in multiple orders of vessel branches under hyperoxic (100% oxygen) and normoxic (21% oxygen) conditions correlate well with the oxygen-hemoglobin dissociation curve. In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength.

Hemoglobin as an important prognostic factor in concurrent chemoradiotherapy for locally advanced carcinoma of the cervix

International Nuclear Information System (INIS)

Toma, Takashi; Nagai, Yutaka; Moromizato, Hidehiko; Toita, Takafumi; Murayama, Sadayuki; Kanazawa, Koji

2005-01-01

The objective of this study was to examine a possible association of hemoglobin with clinical outcome in patients with locally advanced squamous cell carcinoma of the cervix who were treated with concurrent chemoradiotherapy (CCRT). Seventy-five patients with Stage IB to IVA disease who were treated with CCRT were reviewed retrospectively. The mean age was 49.8 years. In the treatment, standard radiotherapy was performed accompanied by concomitant chemotherapy using cisplatin. Pre-treatment hemoglobin was defined as the earliest hemoglobin level prior to the initiation of treatment. Weekly nadir hemoglobin levels throughout treatment were averaged and used as average weekly nadir hemoglobin during treatment (AWNHg). The mean follow-up time was 28.6 months. The mean pre-treatment hemoglobin of 11.6 g/dL was significantly reduced to the mean AWNHg of 9.9 g/dL. The levels of pre-treatment hemoglobin and AWNHg were significantly associated with tumor response to treatment. The 5-year cumulative disease-free survival and overall survival rates for all 75 patients were 67.8% and 75.3%, respectively. Multivariate statistical analysis revealed that AWNHg (≥9.0 versus <9.0 g/dL) was an independent prognostic factor for overall survival (p=0.038), but pre-treatment hemoglobin was not a significant factor. AWNHg was one of the most powerful independent predictors of overall survival in patients undergoing CCRT for locally advanced squamous cell carcinoma of the cervix. (author)

Influence of peripheral blood hemoglobin concentration on the result of radiotherapy for nasopharyngeal carcinoma

International Nuclear Information System (INIS)

He Beiwa; Zhang Guofen; Zhao Yutian; Wang Zhenwu; Xu Min; Hu Yulin

2002-01-01

Objective: To determine the influence of peripheral blood hemoglobin concentration on the radiotherapy result of nasopharyngeal carcinoma (NPC). Methods: From January 1989 to December 1998, 304 patients with pathologically confirmed NPC received radical radiation. There were 209 males and 95 females. The ages ranged from 16 to 77 years with a median of 42. All patients were irradiated by 60 Co or 6 MV external beam with a total dose of 64 - 76 Gy for the primary tumor and 46 - 77 Gy for the cervical lymph nodes. The peripheral blood hemoglobin concentration for all patients was measured before, during and after radiotherapy. These patients were divided into three groups according to the peripheral blood hemoglobin concentration before radiotherapy: anemia ( 160 g/L), and into two groups according to the change in the peripheral blood hemoglobin concentration during radiotherapy as increased and decreased groups. Results: All patients were followed with a follow-up rate of 90.5%. The peripheral blood hemoglobin concentration had a significant effect on the survival of NPC patients. Its decrease or increase during radiotherapy affected the survival and local control rates of NPC patients. Conclusions: The change of peripheral hemoglobin concentration affecting the oxygen content in the blood, can influence the local control and survival rates of NPC patients. Increase results in higher survival

EPR study of manganese(II) binding to 55'-ATP, hemoglobin, and hemocyanin

Energy Technology Data Exchange (ETDEWEB)

Chang, S.S. (Duquesne Univ., Pittsburgh); Li, N.C.; Pratt, D.W.

1975-01-01

Several divalent metal ions affect the oxygen affinity of hemoglobin and hemocyanin. It is important, therefore, to understand the nature of metal-ion binding to these proteins. By comparing the EPR spectra of Mn(II), 0.001 M, in the absence and presence of carboxyhemoglobin or Limulus oxyhemocyanin (pH 7.3, Trizma buffer), the number of Mn binding sites, n, and the binding constant, K, can be determined. For carboxyhemoglobin, HbCO, we find 0.5 Mn binding sites per heme, K = 450 M/sup -1/. Each hemoglobin tetramer therefore binds two manganous ions suggesting that Mn(II), like Cu(II), may bind preferentially to one of the two types of subunits in hemoglobin. For hemocyanin, HcO/sub 2/, we find n = 5.8, K = 1.55 x 10/sup 3/ M/sup -1/. Each oxyhemocyanine therefore binds approximately six manganous ions, and the binding constant is three times larger than that for HbCO. We have also carried out similar experiments on 5'-ATP, and on solutions of HbCO and ATP containing McCl/sub 2/ or ZnCl/sub 2/. Zn(II) effectively competes with Mn(II) in binding hemoglobin and ATP, whereas Mg(II) does not, in accord with expectations from data on oxygen affinity of hemoglobin. (auth)

An analysis of postoperative hemoglobin levels in patients with a fractured neck of femur

OpenAIRE

Navraj S. Nagra; Dmitri van Popta; Sigrid Whiteside; Edward M. Holt

2016-01-01

Objectives: The aim of this study was to analyze the changes in hemoglobin level and to determine a suitable timeline for post-operative hemoglobin monitoring in patients undergoing fixation of femoral neck fracture. Patients and methods: Patients who underwent either dynamic hip screw (DHS) fixation (n = 74, mean age: 80 years) or hip hemiarthroplasty (n = 104, mean age: 84 years) for femoral neck fracture were included into the study. The hemoglobin level of the patients was monitored pe...

U-shaped curve for risk associated with maternal hemoglobin, iron status, or iron supplementation.

Science.gov (United States)

Dewey, Kathryn G; Oaks, Brietta M

2017-12-01

Both iron deficiency (ID) and excess can lead to impaired health status. There is substantial evidence of a U-shaped curve between the risk of adverse birth outcomes and maternal hemoglobin concentrations during pregnancy; however, it is unclear whether those relations are attributable to conditions of low and high iron status or to other mechanisms. We summarized current evidence from human studies regarding the association between birth outcomes and maternal hemoglobin concentrations or iron status. We also reviewed effects of iron supplementation on birth outcomes among women at low risk of ID and the potential mechanisms for adverse effects of high iron status during pregnancy. Overall, we confirmed a U-shaped curve for the risk of adverse birth outcomes with maternal hemoglobin concentrations, but the relations differ by trimester. For low hemoglobin concentrations, the link with adverse outcomes is more evident when hemoglobin concentrations are measured in early pregnancy. These relations generally became weaker or nonexistent when hemoglobin concentrations are measured in the second or third trimesters. Associations between high hemoglobin concentration and adverse birth outcomes are evident in all 3 trimesters but evidence is mixed. There is less evidence for the associations between maternal iron status and adverse birth outcomes. Most studies used serum ferritin (SF) concentrations as the indicator of iron status, which makes the interpretation of results challenging because SF concentrations increase in response to inflammation or infection. The effect of iron supplementation during pregnancy may depend on initial iron status. There are several mechanisms through which high iron status during pregnancy may have adverse effects on birth outcomes, including oxidative stress, increased blood viscosity, and impaired systemic response to inflammation and infection. Research is needed to understand the biological processes that underlie the U-shaped curves

Hemoglobin as an independent prognostic factor in the radiotherapy of head and neck tumors

International Nuclear Information System (INIS)

Schaefer, U.; Micke, O.; Mueller, S.B.; Schueller, P.; Willich, N.

2003-01-01

Purpose: The purpose of this study was to analyze the prognostic value of baseline hemoglobin levels before radiotherapy in patients with head and neck tumors. Patients and Methods: In a retrospective study with a median follow-up of 43 months, we analyzed the results of 214 patients irradiated for head and neck cancer between January 1, 1990 and January 1, 1998 (180 men and 34 women; median age 58 years). The treatment concept consisted in adjuvant radiotherapy in 58 patients, 77 patients received definitive radiochemotherapy, 42 patients definitive radiotherapy, and 37 patients reirradiation for in-field recurrence. Baseline hemoglobin values were divided in four groups of the same patient number (quartiles). Several known prognostic factors like sex, tumor stage, histologic grading, performance status, and treatment scheme were analyzed for their influence on overall and event-free survival and correlated with pretreatment hemoglobin values (Kaplan-Meier method). In addition, univariate und multivariate logistic regression analyses were carried out to evaluate the effect of baseline hemoglobin on response rates. Results: The median survival (event-free survival) of all patients amounted to 15 months (10 months). 25%, 50%, and 75% of patients had hemoglobin values < 11.2 g/dl, < 12.7 g/dl, and < 13.9 g/dl, respectively. In the univariate analysis, the following variables were significant prognostic factors for overall/event-free survival (log-rank test): treatment concept (p < 0.001/ p < 0.001), tumor stage (p < 0.001/p < 0.001), general condition (p < 0.001/p < 0.001), and pretreatment hemoglobin (p = 0.014/p = 0.05). Multivariate analysis (Cox) proved these parameters to be independent of each other. In addition, response rate after radiation showed a strong association between hemoglobin and local control probability (p = 0.02). Conclusion: In this retrospective analysis, baseline hemoglobin level was shown to be an independent significant prognostic factor in

The Use of Dual Forms in the “Collection of Lives of Greek Saints” by Saint Nilus of Sora

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Polina Yuryevna Karavaeva

2015-09-01

Full Text Available In this article we reveal the key principles of dual contexts in Hagiography 'Life of Saint Simeon Stylites of the Admirable Mountain' and 'Life of Saint Onoufrios of the Desert', which are included into the "Collection of Lives of Greek Saints". We proceed from the discrepancy between the history of dual number in colloquial language and book-Slavic language. And we consider the form of dual number as a sign of book-Slavic grammatical norm in the period of the end of 15th - beginning of the 16th centuries. St. Nilus of Sora uses dual number not only with certain stylistic purposes, but also as a clear indication that a text is a monument, that implements strict grammatical norms of Church Slavonic Russian. We provide the most detailed description of the features of the use of free dual forms and also the description of the features of the use of associated dual number. According to the classification, which was proposed by A. Belich, free dual number refers to symmetrical objects, usually of the free dual number are the most stable and consistent in its use. So, one of the features of the representations of St. Nilus of Sora and his disciples about the grammatical norm of using dual number forms is used as a grammatical synonymic forms of the plural and dual number of verb forms in the combination of a noun with a numeral "two".

Rheological Variations among Nigerians with Different Hemoglobin ...

African Journals Online (AJOL)

Some Hemorheological determinants such as whole blood viscosity (WBV) and plasma viscosity (PV) and Plasma Fibrinogen Concentration (PFC) were measured with standard methods. We recorded a relatively unchanged whole blood viscosities in subjects with various hemoglobin genotypes (AA, AS and SS; P>0.05, ...

Electron self-exchange in hemoglobins revealed by deutero-hemin substitution.

Science.gov (United States)

Athwal, Navjot Singh; Alagurajan, Jagannathan; Sturms, Ryan; Fulton, D Bruce; Andreotti, Amy H; Hargrove, Mark S

2015-09-01

Hemoglobins (phytoglobins) from rice plants (nsHb1) and from the cyanobacterium Synechocystis (PCC 6803) (SynHb) can reduce hydroxylamine with two electrons to form ammonium. The reaction requires intermolecular electron transfer between protein molecules, and rapid electron self-exchange might play a role in distinguishing these hemoglobins from others with slower reaction rates, such as myoglobin. A relatively rapid electron self-exchange rate constant has been measured for SynHb by NMR, but the rate constant for myoglobin is equivocal and a value for nsHb1 has not yet been measured. Here we report electron self-exchange rate constants for nsHb1 and Mb as a test of their role in hydroxylamine reduction. These proteins are not suitable for analysis by NMR ZZ exchange, so a method was developed that uses cross-reactions between each hemoglobin and its deutero-hemin substituted counterpart. The resulting electron transfer is between identical proteins with low driving forces and thus closely approximates true electron self-exchange. The reactions can be monitored spectrally due to the distinct spectra of the prosthetic groups, and from this electron self-exchange rate constants of 880 (SynHb), 2900 (nsHb1), and 0.05M(-1) s(-1) (Mb) have been measured for each hemoglobin. Calculations of cross-reactions using these values accurately predict hydroxylamine reduction rates for each protein, suggesting that electron self-exchange plays an important role in the reaction. Copyright © 2015 Elsevier Inc. All rights reserved.

Ludopathy and the employees at saint bonaventure university, Medellín branch, Colombia, 2010

OpenAIRE

Cano Bedoya, Victor Hugo; Universidad de San Buenaventura; Pérez Pérez, Jorge Arturo; Universidad de San Buenaventura, Medellín - Colombia

2010-01-01

This current article consists of a description of the use of game of chance and videogames, by the students, instructors, and employees at saint Bonaventure University, Medellin Branch and its relationship with both medical and psychological dimensions, which include variables like the following: concentration difficulties, change of mood, physical discomfort, preference of games to sports, and relaxed atmosphere as it is produced by games. In this true stage, the results with employees are s...

Candidate Sequence Variants and Fetal Hemoglobin in Children with Sickle Cell Disease Treated with Hydroxyurea

Science.gov (United States)

Green, Nancy S.; Ender, Katherine L.; Pashankar, Farzana; Driscoll, Catherine; Giardina, Patricia J.; Mullen, Craig A.; Clark, Lorraine N.; Manwani, Deepa; Crotty, Jennifer; Kisselev, Sergey; Neville, Kathleen A.; Hoppe, Carolyn; Barral, Sandra

2013-01-01

Background Fetal hemoglobin level is a heritable complex trait that strongly correlates swith the clinical severity of sickle cell disease. Only few genetic loci have been identified as robustly associated with fetal hemoglobin in patients with sickle cell disease, primarily adults. The sole approved pharmacologic therapy for this disease is hydroxyurea, with effects largely attributable to induction of fetal hemoglobin. Methodology/Principal Findings In a multi-site observational analysis of children with sickle cell disease, candidate single nucleotide polymorphisms associated with baseline fetal hemoglobin levels in adult sickle cell disease were examined in children at baseline and induced by hydroxyurea therapy. For baseline levels, single marker analysis demonstrated significant association with BCL11A and the beta and epsilon globin loci (HBB and HBE, respectively), with an additive attributable variance from these loci of 23%. Among a subset of children on hydroxyurea, baseline fetal hemoglobin levels explained 33% of the variance in induced levels. The variant in HBE accounted for an additional 13% of the variance in induced levels, while variants in the HBB and BCL11A loci did not contribute beyond baseline levels. Conclusions/Significance These findings clarify the overlap between baseline and hydroxyurea-induced fetal hemoglobin levels in pediatric disease. Studies assessing influences of specific sequence variants in these and other genetic loci in larger populations and in unusual hydroxyurea responders are needed to further understand the maintenance and therapeutic induction of fetal hemoglobin in pediatric sickle cell disease. PMID:23409025

Is Routine Ordering of Both Hemoglobin and Hematocrit Justifiable?

Science.gov (United States)

Addison, David J.

1966-01-01

In order to assess the value of routine simultaneous hemoglobin and hematocrit determinations, paired determinations in the following groups were studied: (1) 360 consecutive pairs from the hematology laboratory, (2) 95 pairs on general medical patients, (3) 43 pairs on 10 patients with upper gastrointestinal hemorrhage, and (4) 62 pairs on 10 patients with burns. These values were plotted on scatter diagrams. In the 560 pairs only three disparate determinations were found. It is concluded that, in most clinical situations, determination of the hemoglobin or the hematocrit as a screening procedure provides as much useful information as the simultaneous determination of both. PMID:5296947

Disturbance in hemoglobin metabolism and in vivo antimalarial activity of azole antimycotics

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Juan Ricardo Rodrigues

2011-02-01

Full Text Available Plasmodium parasites degrade host hemoglobin to obtain free amino acids, essential for protein synthesis. During this event, free toxic heme moieties crystallize spontaneously to produce a non-toxic pigment called hemozoin or ß-hematin. In this context, a group of azole antimycotics, clotrimazole (CTZ, ketoconazole (KTZ and fluconazole (FCZ, were investigated for their abilities to inhibit ß-hematin synthesis (IßHS and hemoglobin proteolysis (IHbP in vitro. The ß-hematin synthesis was recorded by spectrophotometry at 405 nm and the hemoglobin proteolysis was determined by SDS-PAGE 12.5%, followed by densitometric analysis. Compounds were also assayed in vivo in a malaria murine model. CTZ and KTZ exhibited the maximal effects inhibiting both biochemical events, showing inhibition of β-hematin synthesis (IC50 values of 12.4 ± 0.9 µM and 14.4 ± 1.4 µM respectively and inhibition of hemoglobin proteolysis (80.1 ± 2.0% and 55.3 ± 3.6%, respectively. There is a broad correlation to the in vivo results, especially CTZ, which reduced the parasitemia (%P of infected-mice at 4th day post-infection significantly compared to non-treated controls (12.4 ± 3.0% compared to 26.6 ± 3.7%, p = 0.014 and prolonged the survival days post-infection. The results indicated that the inhibition of the hemoglobin metabolism by the azole antimycotics could be responsible for their antimalarial effect.

The first leg transplant for the treatment of a cancer by Saints Cosmas and Damian.

Science.gov (United States)

Androutsos, G; Diamantis, A; Vladimiros, L

2008-01-01

The most renowned of all medically inclined saints must have been St. Cosmas and St. Damian. They became the special patrons of surgery, due to their miraculous operations. On one occasion, they amputated a cancerous leg and replaced it with another, taken from a Moor who had just died. This dramatic cure was meat and drink for artists, and has been portrayed many times.

HIV and other predictors of serum folate, serum ferritin, and hemoglobin in pregnancy

DEFF Research Database (Denmark)

Friis, Henrik; Gomo, E; Kæstel, Pernille

2001-01-01

BACKGROUND: Folate and iron status and hemoglobin concentrations are important to maternal and infant health. OBJECTIVE: Our goal was to identify predictors of serum folate, serum ferritin, and hemoglobin. DESIGN: This was a cross-sectional study of 1669 pregnant women (22-35 wk of gestation) in ...

Treatment of β-Thalassemia/Hemoglobin E with Antioxidant Cocktails Results in Decreased Oxidative Stress, Increased Hemoglobin Concentration, and Improvement of the Hypercoagulable State

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Orn-uma Yanpanitch

2015-01-01

Full Text Available Studies on the antioxidant treatment for thalassemia have reported variable outcomes. However, treatment of thalassemia with a combination of hydrophobic and hydrophilic antioxidants and an iron chelator has not been studied. This study investigated the effects of antioxidant cocktails for the treatment of β-thalassemia/hemoglobin E (HbE, which is the most common form of β-thalassemia in Southeast Asia. Sixty patients were divided into two groups receiving N-acetylcysteine, deferiprone, and either curcuminoids (CUR or vitamin E (Vit-E, and their hematological parameters, iron load, oxidative stress, and blood coagulation potential were evaluated. Patients were classified as responders if they showed the improvements of the markers of iron load and oxidative stress, otherwise as nonresponders. During treatment, the responders in both groups had significantly decreased iron load, oxidative stress, and coagulation potential and significantly increased antioxidant capacity and hemoglobin concentration. The significantly maximum increase (P<0.01 in hemoglobin concentration was 11% at month 4 in CUR group responders and 10% at month 10 in Vit-E group responders. In conclusion, the two antioxidant cocktails can improve anemia, iron overload, oxidative stress, and hypercoagulable state in β-thalassemia/HbE.

Hemoglobinas anormais e dificuldade diagnóstica Abnormal hemoglobins

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Guilherme G. Leoneli

2000-12-01

Full Text Available As hemoglobinas humanas, com padrão de herança definido geneticamente, apresentam variações polimórficas características dentro de nossa população, na dependência dos grupos raciais que formam cada região. Aparecem sob a forma de variantes de hemoglobinas ou talassemias, sendo mais freqüentes, no Brasil, os tipos variantes S e C e as talassemias alfa e beta, todas na forma heterozigota. Durante o ano de 1999, amostras de sangue de 506 indivíduos com anemia a esclarecer ou que já passaram por alguma triagem de hemoglobinopatias foram encaminhadas ao Centro de Referência de Hemoglobinas da UNESP, para confirmação diagnóstica e submetidas a procedimentos eletroforéticos, análises bioquímicas e citológicas, para caracterização das hemoglobinas anormais. O objetivo do presente estudo foi verificar quais tipos de hemoglobinas anormais apresentam maior dificuldade diagnóstica. As amostras foram provenientes de 24 cidades de doze estados. Os resultados mostraram que 354 indivíduos (69,96% apresentaram hemoglobinas anormais, sendo 30 Hb AS (5,93%, 5 Hb AC (0,98%, 76 sugestivos de talassemia alfa heterozigota (15,02%, 134 sugestivos de talassemia beta heterozigota (26,48% e 109 com outras formas de hemoglobinas anormais (21,54%, que incluem variantes raras e interações de diferentes formas de talassemias e hemoglobinas variantes. Concluiu-se que, apesar da melhoria técnica oferecida atualmente e a constante formação de recursos humanos capacitados, as talassemias em sua forma heterozigota (210 indivíduos -- 41,50% são responsáveis pela maior dificuldade diagnóstica, seguido da caracterização de variantes raras e formas interativas de hemoglobinopatias (109 indivíduos -- 21,54%, sugerindo que se deve aumentar a capacidade de formação de pessoal e as informações a respeito destas alterações genéticas em nossa população.The human hemoglobins, with genetically defined inheritance patterns, have shown

Top-Down-Assisted Bottom-Up Method for Homologous Protein Sequencing: Hemoglobin from 33 Bird Species

Science.gov (United States)

Song, Yang; Laskay, Ünige A.; Vilcins, Inger-Marie E.; Barbour, Alan G.; Wysocki, Vicki H.

2015-11-01

Ticks are vectors for disease transmission because they are indiscriminant in their feeding on multiple vertebrate hosts, transmitting pathogens between their hosts. Identifying the hosts on which ticks have fed is important for disease prevention and intervention. We have previously shown that hemoglobin (Hb) remnants from a host on which a tick fed can be used to reveal the host's identity. For the present research, blood was collected from 33 bird species that are common in the U.S. as hosts for ticks but that have unknown Hb sequences. A top-down-assisted bottom-up mass spectrometry approach with a customized searching database, based on variability in known bird hemoglobin sequences, has been devised to facilitate fast and complete sequencing of hemoglobin from birds with unknown sequences. These hemoglobin sequences will be added to a hemoglobin database and used for tick host identification. The general approach has the potential to sequence any set of homologous proteins completely in a rapid manner.

How did Death become a Saint in Mexico?

DEFF Research Database (Denmark)

Kristensen, Regnar Albæk

2014-01-01

Over the last decade, the cult of La Santa Muerte (St Death) has attracted a remarkable number of followers in Mexico and the USA. Whereas the social context of her devotees, who tend to live on the fringes of society, has attracted ample attention from scholars and journalists, one of the princi......Over the last decade, the cult of La Santa Muerte (St Death) has attracted a remarkable number of followers in Mexico and the USA. Whereas the social context of her devotees, who tend to live on the fringes of society, has attracted ample attention from scholars and journalists, one...... of the principal puzzles is still how a skeleton image of death has come to be seen as a saint by large numbers of Catholics. How is it possible for this figure to embrace such antagonistic qualities as death and sainthood in a Christian context? In this semiotic-material exploration of the image’s genealogy, I...

Inhibition of Corneal Neovascularization with the Combination of Bevacizumab and Plasmid Pigment Epithelium-Derived Factor-Synthetic Amphiphile INTeraction-18 (p-PEDF-SAINT-18 Vector in a Rat Corneal Experimental Angiogenesis Model

Directory of Open Access Journals (Sweden)

Ching-Hsein Chen

2013-04-01

Full Text Available Bevacizumab, a 149-kDa protein, is a recombinant humanized monoclonal antibody to VEGF. PEDF, a 50-kDa glycoprotein, has demonstrated anti-vasopermeability properties. In this study, we demonstrated that the combination of bevacizumab and plasmid pigment epithelium-derived factor-synthetic amphiphile INTeraction-18 (p-PEDF-SAINT-18 has a favorable antiangiogenic effect on corneal NV. Four groups (Group A: 0 μg + 0 μg, B: 0.1 μg + 0.1 μg, C: 1 μg + 1 μg, and D: 10 μg + 10 μg of bevacizumab + p-PEDF-SAINT-18 were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus on the temporal side. Then, 1 μg of p-bFGF-SAINT-18 was prepared and implanted into the rat corneal stroma 1.5 mm from the limbus on the same side. The inhibition of NV was observed and quantified from days 1 to 60. Biomicroscopic examination, western blot analysis and immunohistochemistry were used to analyze the 18-kDa bFGF, 50-kDa PEDF and VEGF protein expression. No inhibition activity for normal limbal vessels was noted. Subconjunctival injection with the combination of bevacizumab and p-PEDF-SAINT-18 successfully inhibited corneal NV. The bFGF and PEDF genes were successfully expressed as shown by western blot analysis, and a mild immune response to HLA-DR was shown by immunohistochemistry. We concluded that the combination of bevacizumab and p-PEDF-SAINT-18 may have more potent and prolonged antiangiogenic effects, making it possible to reduce the frequency of subconjunctival.Bevacizumab, a 149-kDa protein, is a recombinant humanized monoclonalantibody to VEGF. PEDF, a 50-kDa glycoprotein, has demonstrated anti-vasopermeabilityproperties. In this study, we demonstrated that the combination of bevacizumaband plasmid pigment epithelium-derived factor-synthetic amphiphile INTeraction-18(p-PEDF-SAINT-18 has a favorable antiangiogenic effect on corneal NV. Four groups(Group A: 0 μg + 0 μg, B: 0.1 μg + 0.1 μg, C: 1 μg + 1 μg, and

Los juegos antropologicos de Saint-Louis

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Fabrice Delsahut

2011-12-01

Full Text Available En 1904, en medio de la feria de la exposición universal de Saint-Louis, los Juegos olimpicos no pudieron escapar a la ascención de ideologías racistas del principios del siglo XX y contibuyeron a la discusión del mérito atlético de diferentes razas. Los organisadores prepararon competiciones especiales que llamaron en esta ocasión «Jornadas antropológicas» reservadas a los que la América segregacionista del momento consideraba como primitivos. El impacto de los estudios sobre las razas, sobre la manera de pensar en el deporte no fue, al contrario de lo que se esforzaron hacer creer los diferentes presidentes del C.O.I en el curso de las siguientes decadas, un simple avatar del movimiento olimpico. Veremos la influencía de este espectáculo atletico interracial en los Estados Unidos pero tambien en el mundo, particularmente a través del papel de la prensa. Las jornadas antropológicas permitieron indudablemente elaborar una cierta percepción de la otricidad y limitaran la integración de los pueblos autóctonas en la maquina deportiva mundial.

21 CFR 864.7470 - Glycosylated hemoglobin assay.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Glycosylated hemoglobin assay. 864.7470 Section 864.7470 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES HEMATOLOGY AND PATHOLOGY DEVICES Hematology Kits and Packages § 864.7470...

Predictors of hemoglobin in Danish blood donors

DEFF Research Database (Denmark)

Kotzé, Sebastian R; Pedersen, Ole B; Petersen, Mikkel S

2015-01-01

BACKGROUND: It is well known that blood donors are at increased risk of iron deficiency and subsequent development of iron deficiency anemia. We aimed to investigate the effect of factors influencing hemoglobin (Hb) levels. STUDY DESIGN AND METHODS: Initiated in 2010, the Danish Blood Donor Study...

The introduction of Melanoides tuberculata (Mollusca: Thiaridae) to the island of Saint Lucia (West Indies) and its role in the decline of Biomphalaria glabrata, the snail intermediate host of Schistosoma mansoni.

Science.gov (United States)

Pointier, J P

1993-06-01

A malacological survey was carried out in May 1992 in the whole hydrographic system of Saint Lucia 11 years after the end of a biological control programme to eliminate Biomphalaria glabrata, the snail intermediate host of Schistosoma mansoni. A competitor snail, Melanoides tuberculata, was introduced to Saint Lucia in 1978 and field experiments in several habitats were conducted by Prentice between 1978 and 1986. At the present time M. tuberculata is the most common freshwater snail in Saint Lucia. The results of the survey, undertaken in sites where B. glabrata occurred in large populations in the past showed (i) the absence of the snail hosts from seven sites now extensively colonized by the competitor (ii) the presence of B. glabrata in low or very low densities in 17 sites together with the competitor and (iii) the presence of the intermediate hosts in large populations in only two sites where M. tuberculata was absent. These results confirm the positive results observed by Prentice. The presence of another planorbid snail, B. straminea, is reported for the first time in Saint Lucia.

Religious [Out]Laws: An Analysis of the 2008 Fundamentalist Latter-day Saints Raid

OpenAIRE

Ambutter, Cassie Nicole

2014-01-01

In early April 2008, based on a single allegation of child abuse, the state of Texas and Child Protective Services (CPS) executed a raid on the Fundamentalist Latter-day Saints, a polygamous Mormon group living on the "Yearning for Zion" ranch in Eldorado, Texas. The raid involved the immediate removal of over 400 children who were then placed in protective custody in nearby San Angelo. Despite the fact that the initial abuse allegation was determined to be a hoax, the raid was the catalyst f...

Segmented Aperture Interferometric Nulling Testbed (SAINT) II: component systems update

Science.gov (United States)

Hicks, Brian A.; Bolcar, Matthew R.; Helmbrecht, Michael A.; Petrone, Peter; Burke, Elliot; Corsetti, James; Dillon, Thomas; Lea, Andrew; Pellicori, Samuel; Sheets, Teresa; Shiri, Ron; Agolli, Jack; DeVries, John; Eberhardt, Andrew; McCabe, Tyler

2017-09-01

This work presents updates to the coronagraph and telescope components of the Segmented Aperture Interferometric Nulling Testbed (SAINT). The project pairs an actively-controlled macro-scale segmented mirror with the Visible Nulling Coronagraph (VNC) towards demonstrating capabilities for the future space observatories needed to directly detect and characterize a significant sample of Earth-sized worlds around nearby stars in the quest for identifying those which may be habitable and possibly harbor life. Efforts to improve the VNC wavefront control optics and mechanisms towards repeating narrowband results are described. A narrative is provided for the design of new optical components aimed at enabling broadband performance. Initial work with the hardware and software interface for controlling the segmented telescope mirror is also presented.

Status gizi, kadar hemoglobin, ureum, dan kreatinin pasien konseling gizi hemodialisa

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Kristiawan P. A. Nugroho

2018-01-01

Full Text Available ABSTRACTBackground : Patients who are undergoing hemodialysis therapy must know which foods that may be consumed including foods containing animal protein, low potassium, and low salt. The role of nutritionists is needed to provide nutritional counseling related to dietary adjustment. Studies that have been conducted by previous researchers are about diet from hemodialysis patients and the various factors that affect dietary compliance of patients, but no one has been studying the relation related to nutritional status, hemoglobin, ureum, and creatinine levels before and after doing a counseling that also has a relationship with diet and dietary compliance for hemodialysis patients.Objectives : To analyze the relationship of nutritional status, hemoglobin, ureum, and creatinine levels of hemodialysis patients before and after doing nutritional counseling in RSUD Ungaran. Methods : The study used a descriptive quantitaive method with one group pretest posttest design with cross sectional approach. The research was conducted in Hemodialysis Unit of RSUD Ungaran with a total sampling of 30 hemodialysis patient. Primary data were obtained from respondent’s data entry and FFQ, observation, and interviews. Secondary data were obtained from respondent’s medical records include the level of hemoglobin, urea, and cretinine. Data were analyzed using SPSS programme with Paired t Test. Results : The results of SPSS analysis showed that probability value from urea men 0,016 < 0,05 and urea women – hemoglobin men and women – creatinin men and women overall 0,000 < 0,05, which means that the average levels of those components before and after doing a nutritional counseling is different.Conclusions : A nutritional counseling indicate any change in the nutritional status of the entire hemodialysis patients, based on hemoglobin levels increased, while urea and creatinine levels decreased; but all those components are not in the normal category. Levels of

An Innovative Simple Technique of Blood Conservation in Adult Patients with Tetralogy of Fallot and Severely Raised Hemoglobin

OpenAIRE

Neema, Praveen Kumar; Manikandan, Sethuraman; Rathod, Ramesh Chandra

2007-01-01

The adult patients of tetralogy of Fallot often present with high hemoglobin levels. High hemoglobin and hematocrit on cardiopulmonary bypass (CPB) are associated with increased hemolysis, plasma free hemoglobin, renal dysfunction or failure, postoperative bleeding, exploration for bleeding, and increased requirement of allogeneic blood and blood products. Despite the presence of high hemoglobin and its association with adverse outcome, blood conservation is rarely practiced in these patients...

Effect of hemolysis and free hemoglobin on optical hematocrit measurements in the extracorporeal circulation.

Science.gov (United States)

Paluszkiewicz, Aleksandra; Kellner, Josef; Elshehabi, Morad; Schneditz, Daniel

2008-01-01

Clinically significant hemolysis is a rare but serious problem in dialysis. Because hemolysis affects red blood cell count and optical density of plasma it has been speculated whether techniques used for online blood volume monitoring would be useful to detect hemolysis. In this study the influence of free hemoglobin on hematocrit and relative blood volume changes measured by optical means (CritLine, HemaMetrics, Kaysville, UT) were examined using an in vitro model with bovine blood. Free hemoglobin solutions were added in steps to circulating whole blood at baseline hematocrits covering a range from 30% to 60% and at blood flows of approximately 200 and 400 ml/min. The free hemoglobin concentration reached was in the range of 2 to 3 g/dl. The presence of free hemoglobin led to a relative increase in hematocrit in the range of 0.3% per 0.1 g of free hemoglobin per dl (+3% dl/g). As an increase in hematocrit is interpreted as a decrease in blood volume, this change referred to an apparent decrease in relative blood volume in the same order of magnitude (-3% dl/g). Effects were more pronounced at low baseline hematocrit. Thus, although optical hematocrit readings are affected by the presence of free hemoglobin the changes at levels associated with clinical symptoms appear to be too small to be accurately detected in the in vivo situation where the hematocrit and the resulting optical signal is affected by various physiological processes and therefore much noisier.

Saint-Laurent-des-Eaux nuclear facilities. 2009 annual report

International Nuclear Information System (INIS)

2010-01-01

This annual report is established on account of article 21 of the 2006-686 French law from June 13, 2006, relative to the transparency and safety in the nuclear domain. It describes, first, the nuclear facilities of Saint-Laurent-des-Eaux, and then the measures taken to ensure their safety (personnel radioprotection, actions implemented for nuclear safety improvement, organisation in crisis situation, external and internal controls, technical assessment of the facilities, administrative procedures carried out in 2009), incidents and accidents registered in 2009, radioactive and chemical effluents released by the facilities in the environment, other pollutions, management of radioactive wastes, and, finally, the actions carried out in the domain of transparency and public information. A glossary and the viewpoint of the Committee of Hygiene, safety and working conditions about the content of the document conclude the report. (J.S.)

Evaluation of the Efficiency of the Reticulocyte Hemoglobin Content on Diagnosis for Iron Deficiency Anemia in Chinese Adults

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Jie Cai

2017-05-01

Full Text Available Our aim was to evaluate the cut-off value and efficiency of using reticulocyte hemoglobin content as a marker to diagnose iron deficiency anemia in Chinese adults. 140 adults who needed bone marrow aspiration for diagnosis at the hematology department of the Peking Union Medical College Hospital were enrolled according to the inclusive and exclusive criteria. Venous blood samples were collected to detect complete blood count, including hemoglobin, reticulocyte hemoglobin content, hematocrit, mean cellular volume, corpuscular hemoglobin concentration, hemoglobin content, free erythrocyte protoporphyrin; iron indexes of serum ferritin, serum transferrin receptor, and unsaturated iron-binding capacity; and inflammation markers of C-reactive protein and α-acid glycoprotein. Bone marrow samples were obtained for the bone marrow iron staining, which was used as the standard for the evaluation of iron status in this study. Subjects were divided into three groups according to hemoglobin levels and bone marrow iron staining results: the IDA (iron deficiency anemia group, the NIDA (non-iron deficiency anemia group, and the control group. The differences of the above-mentioned indexes were compared among the three groups and the effect of inflammation was also considered. The cut-off value of reticulocyte hemoglobin content was determined by receiver operation curves. The IDA group (n = 56 had significantly lower reticulocyte hemoglobin content, mean cellular volume, corpuscular hemoglobin concentration, hemoglobin content, and serum ferritin; and higher free erythrocyte protoporphyrin, unsaturated iron-binding capacity, and serum transferrin receptor (p < 0.05 compared with the NIDA group (n = 38 and control group (n = 46. Hematocrit, serum ferritin, and unsaturated iron-binding capacity were significantly affected by inflammation while reticulocyte hemoglobin content and other parameters were not. The cut-off value of reticulocyte hemoglobin content for

Evaluation of the Efficiency of the Reticulocyte Hemoglobin Content on Diagnosis for Iron Deficiency Anemia in Chinese Adults.

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Cai, Jie; Wu, Meng; Ren, Jie; Du, Yali; Long, Zhangbiao; Li, Guoxun; Han, Bing; Yang, Lichen

2017-05-02

Our aim was to evaluate the cut-off value and efficiency of using reticulocyte hemoglobin content as a marker to diagnose iron deficiency anemia in Chinese adults. 140 adults who needed bone marrow aspiration for diagnosis at the hematology department of the Peking Union Medical College Hospital were enrolled according to the inclusive and exclusive criteria. Venous blood samples were collected to detect complete blood count, including hemoglobin, reticulocyte hemoglobin content, hematocrit, mean cellular volume, corpuscular hemoglobin concentration, hemoglobin content, free erythrocyte protoporphyrin; iron indexes of serum ferritin, serum transferrin receptor, and unsaturated iron-binding capacity; and inflammation markers of C-reactive protein and α-acid glycoprotein. Bone marrow samples were obtained for the bone marrow iron staining, which was used as the standard for the evaluation of iron status in this study. Subjects were divided into three groups according to hemoglobin levels and bone marrow iron staining results: the IDA (iron deficiency anemia) group, the NIDA (non-iron deficiency anemia) group, and the control group. The differences of the above-mentioned indexes were compared among the three groups and the effect of inflammation was also considered. The cut-off value of reticulocyte hemoglobin content was determined by receiver operation curves. The IDA group ( n = 56) had significantly lower reticulocyte hemoglobin content, mean cellular volume, corpuscular hemoglobin concentration, hemoglobin content, and serum ferritin; and higher free erythrocyte protoporphyrin, unsaturated iron-binding capacity, and serum transferrin receptor ( p iron-binding capacity were significantly affected by inflammation while reticulocyte hemoglobin content and other parameters were not. The cut-off value of reticulocyte hemoglobin content for diagnosing iron deficiency anemia was 27.2 pg, with a sensitivity of 87.5% and a specificity of 92.9%. The cut-off values for

Molecular dynamics simulations indicate that deoxyhemoglobin, oxyhemoglobin, carboxyhemoglobin, and glycated hemoglobin under compression and shear exhibit an anisotropic mechanical behavior.

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Yesudasan, Sumith; Wang, Xianqiao; Averett, Rodney D

2018-05-01

We developed a new mechanical model for determining the compression and shear mechanical behavior of four different hemoglobin structures. Previous studies on hemoglobin structures have focused primarily on overall mechanical behavior; however, this study investigates the mechanical behavior of hemoglobin, a major constituent of red blood cells, using steered molecular dynamics (SMD) simulations to obtain anisotropic mechanical behavior under compression and shear loading conditions. Four different configurations of hemoglobin molecules were considered: deoxyhemoglobin (deoxyHb), oxyhemoglobin (HbO 2 ), carboxyhemoglobin (HbCO), and glycated hemoglobin (HbA 1C ). The SMD simulations were performed on the hemoglobin variants to estimate their unidirectional stiffness and shear stiffness. Although hemoglobin is structurally denoted as a globular protein due to its spherical shape and secondary structure, our simulation results show a significant variation in the mechanical strength in different directions (anisotropy) and also a strength variation among the four different hemoglobin configurations studied. The glycated hemoglobin molecule possesses an overall higher compressive mechanical stiffness and shear stiffness when compared to deoxyhemoglobin, oxyhemoglobin, and carboxyhemoglobin molecules. Further results from the models indicate that the hemoglobin structures studied possess a soft outer shell and a stiff core based on stiffness.

[Influence of mastication on the amount of hemoglobin in human brain tissue].

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Sasaki, A

2001-03-01

The purpose of this study was to investigate the influence of mastication on the amount of hemoglobin in human brain tissue. Nine healthy volunteers (6 males and 3 females) participated in this study. They underwent two tasks: 1) at rest, 2) gum-chewing. In seven of the nine (4 males and 3 females), experimental occlusal interference was applied to the first molar of the mandibule on the habitual masticatory side. They underwent the gum-chewing task. To evaluate the amount of hemoglobin, both the hemoglobin oxygenation state and blood volume during gum-chewing were measured in the frontal region, using near-infrared spectroscopy. The amount of total-hemoglobin (blood volume) and oxyhemoglobin of subjects significantly increased during gum-chewing (p experimental occlusal interference was imposed on the subject, the amount of them significantly decreased compared with subjects without experimental occlusal interference (p < 0.05). The results suggested that increases of cerebral blood flow in the frontal region were not due to the mandibular movement, and that human brain activity caused by mastication was not only in the cortical masticatory area but also in the frontal region.

An innovative simple technique of blood conservation in adult patients with tetralogy of Fallot and severely raised hemoglobin.

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Neema, Praveen Kumar; Manikandan, Sethuraman; Rathod, Ramesh Chandra

2007-12-01

The adult patients of tetralogy of Fallot often present with high hemoglobin levels. High hemoglobin and hematocrit on cardiopulmonary bypass (CPB) are associated with increased hemolysis, plasma free hemoglobin, renal dysfunction or failure, postoperative bleeding, exploration for bleeding, and increased requirement of allogeneic blood and blood products. Despite the presence of high hemoglobin and its association with adverse outcome, blood conservation is rarely practiced in these patients because of the fear of possible hemodynamic instability, and hypoxemic spell. We describe an innovative, simple technique of blood conservation for adult patients of tetralogy of Fallot with severely raised hemoglobin. With this technique, hemoglobin can be normalized on CPB; moreover, there is no fear of hypoxemic spell or hemodynamic instability. Furthermore, the blood conserved is readily available for transfusion in the perioperative period, if needed.

HEMOGLOBINA GLICOSILADA O HEMOGLOBINA GLICADA, ¿CUÁL DE LAS DOS? | GLYCOSILATED HEMOGLOBIN OR GLYCATED HEMOGLOBIN, WHICH OF THE TWO?

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Mariela Bracho-Nava

2015-11-01

, according to the International Federation of Clinical Chemistry (IFCC, as a generic term referring to a group of substances that are formed from biochemical reactions between hemoglobin A (HbA and some reducing sugars present in the bloodstream, glucose being the most abundant of them. This reaction is known as the Maillard reaction, which is based on a non-enzimatic glycosylation, or more correctly called, in a glycation. Custom, ignorance or confusion among both chemical processes has led to use the term glycosylated hemoglobin instead of glycated hemoglobin. This article provides a review of the process of formation of hemoglobin A1c, defining the reaction of glycosylation and the protein glycation, the chemical species that favor the glycation, the characteristics of the process of glycation of hemoglobin, stages in which it occurs and the effects related to the glycation of proteins in human beings, to finally conclude with a passage of designations which has received the HbA1c to the present; all with the aim of clarifying and giving property to the use of the term glycated hemoglobin.