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Sample records for hemiptera aleyrodidae em

  1. The complete mitochondrial genome of a spiraling whitefly, Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae).

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    Ming-Xing, Lu; Zhi-Teng, Chen; Wei-Wei, Yu; Yu-Zhou, Du

    2017-03-01

    We report the complete mitochondrial genome (mitogenome) of a spiraling whitefly, Aleurodicus dispersus (Hemiptera: Aleyrodidae). The 16 170 bp long genome consists of 13 protein-coding genes, 20 transfer RNAs, 2 ribosomal RNAs, and a control region. The A. dispersus mitogenome also includes a cytb-like non-coding region and shows several variations relative to the typical insect mitogenome. A phylogenetic tree has been constructed using the 13 protein-coding genes of 12 related species from Hemiptera. Our results would contribute to further study of phylogeny in Aleyrodidae and Hemiptera.

  2. Mitigating trans-boundary movement of Bemisia tabaci (Hemiptera: Aleyrodidae) on Mentha sp. by pre-shipping treaments of biopesticides

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    Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a major pest of several important crops including vegetables, cereals, fruits, and ornamentals grown worldwide. One important mode of its dispersal is through the trans-boundary movement of infested plant materials. In order to prevent the sprea...

  3. Identifikasi kutukebul (Hemiptera: Aleyrodidae dari beberapa tanaman inang dan perkembangan populasinya

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    Purnama Hidayat

    2017-02-01

    Full Text Available Whiteflies (Hemiptera: Aleyrodidae can cause direct and indirect damages on plants, especially vegetables. There is only limited information regarding taxonomy and population dynamic of whiteflies attacking vegetables in Indonesia. This research is conducted to identify species of whitefly collected from chili pepper, tomato, and soybean, and to study their population dynamic. The information gathered from these studies will be useful to support whitefly management in the field. Based on morphology identification of the puparium collected directly from the host plants, there were four species of whitefly identified from chili pepper, tomato, and soybean in Bogor, Cianjur, and Sukabumi, i.e. Bemisia tabaci, Aleurodicus dispersus, Trialeurodes vaporariorum, and Dialeurodes sp. The presence of B. tabaci on chili pepper and tomato was associated with virus infection that causes yellowing and leaf curl disease. This population of B. tabaci tended to increase along with plant growth and generally reached the highest population when the plant was 60-70 days after planting.

  4. Ocorrência de mosca-branca em Annona squamosa no estado de São Paulo

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    Maria José De Marchi Garcia

    2013-12-01

    Full Text Available No Brasil, é restrito o conhecimento sobre distribuição geográfica e plantas hospedeiras de moscas-brancas (Hemiptera: Aleyrodidae, principalmente em plantas cultivadas. Nesta comunicação, é registrada a ocorrência de Aleurodicus mirabilis (Cockerell, 1898 em altas infestações em fruta-do-conde (Annona squamosa Linnaeus, 1753 no estado de São Paulo, Brasil.

  5. Populations of predators and parasitoids of Bemisia tabaci (Hemiptera: Aleyrodidae) after the application of eight biorational insecticides in vegetable crops.

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    Simmons, Alvin M; Shaaban, Abd-Rabou

    2011-08-01

    The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is an important pest of vegetables and many other crops worldwide. Eight biorational insecticides (based on oil, plant derivatives, insect growth regulator and fungus) were evaluated in the field for their influence on populations of six natural enemies of B. tabaci. Natural populations of two predators [Chrysoperla carnea Stephen (Neuroptera: Chrysopidae) and Orius spp. (Hemiptera: Anthocoridae)] and two genera of parasitoids [Encarsia spp. and Eretmocerus spp. (Hymenoptera: Aphelinidae)] were evaluated in eggplant (Solanum melongena L.). Also, augmented field populations of three predators [C. carnea, Coccinella undecimpunctata L. (Coleoptera: Coccinellidae) and Macrolophus caliginosus (Wagner) (Hemiptera: Miridae)] were evaluated in cabbage (Brassica oleracea var. capitata L.), cucumber (Cucumis sativus L.) and squash (Cucurbita pepo L.). Regardless of natural enemy or crop, jojoba oil, Biovar and Neemix had the least effect on abundance of the natural enemies in comparison with the other insecticides during a 14 day evaluation period. Conversely, Admiral, KZ oil, Mesrona oil, Mesrona oil + sulfur and natural oil had a high detrimental effect on abundance of the natural enemies. These results demonstrate the differential effects of biorational insecticides for whitefly control on predators and parasitoids in the field. This article is a US Government work and is in the public domain in the USA. Published 2011 by John Wiley & Sons, Ltd.

  6. Efeito inseticida sistêmico de nanoformulações à base de nim sobre Bemisia tabaci (Hemiptera: Aleyrodidae biótipo B em tomateiro

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    Sheila Salles de Carvalho

    2015-01-01

    Full Text Available Este estudo avaliou a ação inseticida sistêmica e o efeito residual de nanoformulações à base de derivados de nim (Azadirachta indica A. Juss, Meliaceae sobre ninfas de Bemisia tabaci biótipo B (Hemiptera: Aleyrodidae, espécie-praga de diversas culturas de importância econômica. Suspensões coloidais contendo nanocápsulas carregadas com produtos derivados de A. indica foram preparadas utilizando polímeros biodegradáveis como poli-ε-caprolactona (PCL e poli-β-hidroxibutirato (PHB. A matriz encapsulada era composta por uma formulação comercial contendo uma quantidade enriquecida e conhecida dos limonoides azadiractina e 3-tigloilazadiractol (Azamax® 1,2 CE. Inicialmente foi estimada a CL50, para ninfas de B. tabaci, de soluções contendo óleo de nim, que foram aplicadas via água de irrigação (solo em tomateiro. Posteriormente, dois lotes de nanoformulações, com variação no tipo e quantidade dos polímeros e na quantidade do óleo comercial, foram testados quanto à ação sistêmica. As nanoformulações NC L5-2 (com nanocápsulas de PCL e NC L6-1 (com nanocápsulas de PHB, as mais eficazes entre as testadas, foram avaliadas quanto ao efeito residual. A CL50 estimada foi de 180,67 mg L–1 de azadiractina. Constatou-se também que a ação sistêmica de óleos e nanoformulações contendo compostos de nim dependem das condições ambientais em que são aplicados. O Azamax® não apresenta efeito deterrente sobre a oviposição, nem age sobre o desenvolvimento embrionário da mosca-branca quando aplicado via água de irrigação no solo no momento da infestação. As nanoformulações selecionadas são bioativas mesmo cerca de 30 dias após a aplicação, não diferindo do produto comercial.

  7. Distribuição espacial de Aphis gossypii (Glover (Hemiptera, Aphididae e Bemisia tabaci (Gennadius biótipo B (Hemiptera, Aleyrodidae em algodoeiro Bt e não-Bt Spatial distribution of Aphis gossypii (Glover (Hemiptera, Aphididae and Bemisia tabaci (Gennadius biotype B (Hemiptera, Aleyrodidae on Bt and non-Bt cotton

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    Tatiana Rojas Rodrigues

    2010-03-01

    Full Text Available Distribuição espacial de Aphis gossypii (Glover (Hemiptera, Aphididae e Bemisia tabaci (Gennadius biótipo B (Hemiptera, Aleyrodidae em algodoeiro Bt e não-Bt. O estudo da distribuição espacial de adultos de Bemisia tabaci e de Aphis gossypii nas culturas do algodoeiro Bt e não-Bt é fundamental para a otimização de técnicas de amostragens, além de revelar diferenças de comportamento de espécies não-alvo dessa tecnologia Bt entre as duas cultivares. Nesse sentido, o experimento buscou investigar o padrão da distribuição espacial dessas espécies de insetos no algodoeiro convencional não-Bt e no cultivar Bt. As avaliações ocorreram em dois campos de 5.000 m² cada, nos quais se realizou 14 avaliações com contagem de adultos da mosca-branca e colônias de pulgões. Foram calculados os índices de agregação (razão variância/média, índice de Morisita e Expoente k da Distribuição Binomial Negativa e realizados os testes ajustes das classes numéricas de indivíduos encontradas e esperadas às distribuições teóricas de freqüência (Poisson, Binomial Negativa e Binomial Positiva. Todas as análises mostraram que, em ambas as cultivares, a distribuição espacial de B. tabaci ajustou-se a distribuição binomial negativa durante todo o período analisado, indicando que a cultivar transgênica não influenciou o padrão de distribuição agregada desse inseto. Já com relação às análises para A. gossypii, os índices de agregação apontaram distribuição agregada nas duas cultivares, mas as distribuições de freqüência permitiram concluir a ocorrência de distribuição agregada apenas no algodoeiro convencional, pois não houve nenhum ajuste para os dados na cultivar Bt. Isso indica que o algodão Bt alterou o padrão normal de dispersão dos pulgões no cultivo.The study of spatial distribution of the adults of Bemisia tabaci and the colonies of Aphis gossypii on Bt and non-Bt cotton crop is fundamental for

  8. Spatiotemporal Dynamics of Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) in Commercial Watermelon Crops.

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    Lima, Carlos H O; Sarmento, Renato A; Galdino, Tarcísio V S; Pereira, Poliana S; Silva, Joedna; Souza, Danival J; Dos Santos, Gil R; Costa, Thiago L; Picanço, Marcelo C

    2018-04-16

    Spatiotemporal dynamics studies of crop pests enable the determination of the colonization pattern and dispersion of these insects in the landscape. Geostatistics is an efficient tool for these studies: to determine the spatial distribution pattern of the pest in the crops and to make maps that represent this situation. Analysis of these maps across the development of plants can be used as a tool in precision agriculture programs. Watermelon, Citrullus lanatus (Thunb.) Matsum. and Nakai (Cucurbitales: Cucurbitaceae), is the second most consumed fruit in the world, and the whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is one of the most important pests of this crop. Thus, the objective of this work was to determine the spatiotemporal distribution of B. tabaci in commercial watermelon crops using geostatistics. For 2 yr, we monitored adult whitefly densities in eight watermelon crops in a tropical climate region. The location of the samples and other crops in the landscape was georeferenced. Experimental data were submitted to geostatistical analysis. The colonization of B. tabaci had two patterns. In the first, the colonization started at the outermost parts of the crop. In the second, the insects occupied the whole area of the crop since the beginning of cultivation. The maximum distance between sites of watermelon crops in which spatial dependence of B. tabaci densities was observed was 19.69 m. The adult B. tabaci densities in the eight watermelon fields were positively correlated with rainfall and relative humidity, whereas wind speed negatively affected whiteflies population.

  9. Nitrogen in Hydroponic Growing Medium of Tomato Affects the Demographic Parameters of Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae).

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    Hosseini, R S; Madadi, H; Hosseini, M; Delshad, M; Dashti, F

    2015-12-01

    We evaluated the effects of different nitrogen levels (380, 310, 240, and 174 ppm) on the life history parameters of Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae) on hydroponically cultured tomato plants. Our data show that there is a positive correlation between the nitrogen content and the demographic parameters, as the intrinsic rate of increase of T. vaporariorum was the lowest (0.059 ± 0.007 day(-1)) at 174 ppm and the highest (0.088 ± 0.005 day(-1)) at 380 ppm of nitrogen. The net reproduction rate (R 0), finite rate of increase (λ), and mean developmental time (T) were significantly influenced by the nitrogen levels. The mean longevity of males and females showed a positive relationship with the nitrogen level, ranging from 64.8 ± 3.96 to 76.3 ± 2.44 for males and 61.6 ± 5.35 to 71.2 ± 2.44 for females, observed in the lowest and highest nitrogen levels, respectively. The relationship between nitrogen fertilization and T. vaporariorum management on tomato crops is discussed.

  10. Facultative symbiont Hamiltonella confers benefits to Bemisia tabaci (Hemiptera: Aleyrodidae), an invasive agricultural pest worldwide.

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    Su, Qi; Oliver, Kerry M; Pan, Huipeng; Jiao, Xiaoguo; Liu, Baiming; Xie, Wen; Wang, Shaoli; Wu, Qingjun; Xu, Baoyun; White, Jennifer A; Zhou, Xuguo; Zhang, Youjun

    2013-12-01

    Bacterial symbionts infect most insect species, including important pests such as whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), and often exert important effects on host ecology. The facultative symbiont Hamiltonella is found at high frequencies in the B. tabaci MED (type: Mediterranean-MED) in China. The prevalence of this symbiont in natural populations suggests beneficial effects of infection or manipulation of host reproduction. To date, however, no empirical studies on the biological role of Hamiltonella on the host B. tabaci have been reported. Here, we investigated the effects of Hamiltonella infection on the sex ratio and several fitness parameters in B. tabaci MED by comparing Hamiltonella-infected whiteflies with Hamiltonella-free ones. We found that Hamiltonella-infected whiteflies produced significantly more eggs, exhibited significantly higher nymphal survival, faster development times, and larger adult body size in comparison with Hamiltonella-free whiteflies, while no evidence of reproductive manipulation by Hamiltonella were found in B. tabaci MED. In conclusion, Hamiltonella infection substantially enhanced B. tabaci MED performance. This beneficial role may, at least partially, explain the high prevalence of Hamiltonella in B. tabaci MED populations and may also contribute to their effectiveness in spread of the plant pathogens tomato yellow leaf curl virus.

  11. Characterization of a Newly Discovered Symbiont of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)

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    Bing, Xiao-Li; Yang, Jiao; Zchori-Fein, Einat; Wang, Xiao-Wei

    2013-01-01

    Bemisia tabaci (Hemiptera: Aleyrodidae) is a species complex containing >28 cryptic species, some of which are important crop pests worldwide. Like many other sap-sucking insects, whiteflies harbor an obligatory symbiont, “Candidatus Portiera aleyrodidarum,” and a number of secondary symbionts. So far, six genera of secondary symbionts have been identified in B. tabaci. In this study, we report and describe the finding of an additional bacterium in the indigenous B. tabaci cryptic species China 1 (formerly known as B. tabaci biotype ZHJ3). Phylogenetic analysis based on the 16S rRNA and gltA genes showed that the bacterium belongs to the Alphaproteobacteria subdivision of the Proteobacteria and has a close relationship with human pathogens of the genus Orientia. Consequently, we temporarily named it Orientia-like organism (OLO). OLO was found in six of eight wild populations of B. tabaci China 1, with the infection rate ranging from 46.2% to 76.8%. Fluorescence in situ hybridization (FISH) of B. tabaci China 1 in nymphs and adults revealed that OLOs are confined to the bacteriome and co-occur with “Ca. Portiera aleyrodidarum.” The vertical transmission of OLO was demonstrated by detection of OLO at the anterior pole end of the oocytes through FISH. Quantitative PCR analysis of population dynamics suggested a complex interaction between “Ca. Portiera aleyrodidarum” and OLO. Based on these results, we propose “Candidatus Hemipteriphilus asiaticus” for the classification of this symbiont from B. tabaci. PMID:23144129

  12. Resposta de fêmeas de Encarsia formosa Gahan (Hymenoptera: Aphelinidae aos odores do hospedeiro e da planta-hospedeira em olfatômetro de quatro vias Response of female Encarsia formosa Gahan (Hymenoptera: Aphelinidae to host and plant-host odors

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    Kátia Maria Medeiros de Siqueira

    2003-01-01

    Full Text Available A crescente importância da mosca-branca Bemisia tabaci raça B (Hemiptera: Aleyrodidae como praga agrícola tem incentivado a busca de inimigos naturais que possam ser utilizados em programas de controle biológico. Estudou-se a atração de fêmeas de Encarsia formosa (Hymenoptera: Aphelinidae aos odores emanados pelo seu hospedeiro - a mosca-branca B. tabaci raça B - em plantas de tomate, em olfatômetro de quatro vias. O parasitóide não apresentou atração aos odores da planta de tomate nem ao complexo planta de tomate-ninfas de B. tabaci.The increasing importance of the whitefly Bemisia tabaci race B (Hemiptera: Aleyrodidae as one of the major agricultural pest of this century, has resulted in a search for natural enemies that can be used in biological control programs. The response of naive females of Encarsia formosa (Hymenoptera: Aphelinidaeto volatiles from its hostspecies: insect (B. tabacci race B and plant (Lycopersicom esculentum Mill. were tested using 4-nose olfactometre. Parasitoid was not attracted by neither or insect hostspecies volatile.

  13. Cryptic Species Identification and Composition of Bemisia tabaci (Hemiptera: Aleyrodidae) Complex in Henan Province, China

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    Hu, Jian; Wang, Lun-Ji; Dong, Jun-Feng; Song, Yue-Qin; Sun, Hui-Zhong

    2017-01-01

    Abstract Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cryptic species complex, causing significant crop losses in China during the last decade. Although knowledge of cryptic species composition and dynamics within B. tabaci complex is critical for developing sustainable pest management strategies, limited information is available on this pest in the Henan province of China. A systematic survey of the cryptic species composition and distribution of B. tabaci complex in different locations of Henan province was conducted in 2012. The results of RAPD-PCR and the gene for the mitochondrial cytochrome oxidase subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian method indicated there were four known cryptic species MEAM1, MED, Asia II 3, Asia II 9 and a new cryptic species named China 6 in Henan province. In the survey, the invasive cryptic species MED and MEAM1 were found to be predominant with wide spread distribution across the surveyed regions. On the contrary, the indigenous B. tabaci cryptic species including Asia II 3, Asia II 9 and China 6 remained with low prevalence in some surveyed regions. Cryptic species MEAM1 and MED have not completely displaced the native B. tabaci in Henan province. This current study for the first time unifies our knowledge of the diversity and distribution of B. tabaci across Henan province of China. PMID:28973577

  14. Preferência de Bemisia tabaci biótipo B em linhagens mutantes de algodoeiro Bemisia tabaci biotype B preference in mutant cotton lines

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    Francisco das Chagas Vidal Neto

    2008-02-01

    Full Text Available Os efeitos de caracteres mutantes morfológicos do algodoeiro (Gossypium hirsutum L. r. latifolium Hutch.: folha okra, bráctea frego e planta vermelha, em relação à resistência à mosca-branca (Bemisia tabaci biótipo B Hemiptera: Aleyrodidae, foram avaliados em experimentos com ou sem chance de escolha. Os experimentos foram conduzidos em casa-de-vegetação, no delineamento de blocos ao acaso, em fatorial 23 + 1, com quatro repetições. O mutante com a característica planta vermelha foi menos atrativo e menos preferido para oviposição, em relação à planta verde, em ambos os ensaios, com ou sem escolha. Não houve preferência quanto à forma da folha e ao tipo de bráctea.The effects of cotton lines (Gossypium hirsutum L. r. latifolium Hutch. with mutants morphologic characteristics: okra leaf, frego bract and red plant in relation to host plant resistance to whitefly (Bemisia tabaci bioyipe B Hemiptera: Aleyrodidae, were evaluated in choice or no choice assays. The assays were carried out in the greenhouse conditions, according to a completely randomized block design, in a 23 + 1 in a factorial arrangement with four replications. The mutant with red plant characteristic was less attractive and less preferred for oviposition than the normal green plant does, in both, whit or without choice tests. It did not have preference in relation to the form of the leaf and bract type.

  15. Cryptic Species Identification and Composition of Bemisia tabaci (Hemiptera: Aleyrodidae) Complex in Henan Province, China.

    Science.gov (United States)

    Jiu, Min; Hu, Jian; Wang, Lun-Ji; Dong, Jun-Feng; Song, Yue-Qin; Sun, Hui-Zhong

    2017-05-01

    Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cryptic species complex, causing significant crop losses in China during the last decade. Although knowledge of cryptic species composition and dynamics within B. tabaci complex is critical for developing sustainable pest management strategies, limited information is available on this pest in the Henan province of China. A systematic survey of the cryptic species composition and distribution of B. tabaci complex in different locations of Henan province was conducted in 2012. The results of RAPD-PCR and the gene for the mitochondrial cytochrome oxidase subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian method indicated there were four known cryptic species MEAM1, MED, Asia II 3, Asia II 9 and a new cryptic species named China 6 in Henan province. In the survey, the invasive cryptic species MED and MEAM1 were found to be predominant with wide spread distribution across the surveyed regions. On the contrary, the indigenous B. tabaci cryptic species including Asia II 3, Asia II 9 and China 6 remained with low prevalence in some surveyed regions. Cryptic species MEAM1 and MED have not completely displaced the native B. tabaci in Henan province. This current study for the first time unifies our knowledge of the diversity and distribution of B. tabaci across Henan province of China. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America.

  16. Taxonomic status of the Bemisia tabaci complex (Hemiptera: Aleyrodidae and reassessment of the number of its constituent species.

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    Wonhoon Lee

    Full Text Available Bemisia tabaci (Hemiptera: Aleyrodidae is one of the most important insect pests in the world. In the present study, the taxonomic status of B. tabaci and the number of species composing the B. tabaci complex were determined based on 1059 COI sequences of B. tabaci and 509 COI sequences of 153 hemipteran species. The genetic divergence within B. tabaci was conspicuously higher (on average, 11.1% than interspecific genetic divergence within the respective genera of the 153 species (on average, 6.5%. This result indicates that B. tabaci is composed of multiple species that may belong to different genera or subfamilies. A phylogenetic tree constructed based on 212 COI sequences without duplications revealed that the B. tabaci complex is composed of a total of 31 putative species, including a new species, JpL. However, genetic divergence within six species (Asia II 1, Asia II 7, Australia, Mediterranean, New World, and Sub Saharan Africa 1 was higher than 3.5%, which has been used as a threshold of species boundaries within the B. tabaci complex. These results suggest that it is necessary to increase the threshold for species boundaries up to 4% to distinguish the constituent species in the B. tabaci complex.

  17. Fatores que influenciam o ataque de mosca-branca em jiloeiro Factors affecting attack rate of the whitefly on Solanum gilo

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    Germano Leão Demolin Leite

    2002-07-01

    Full Text Available Este trabalho objetivou determinar os efeitos de pluviosidade e temperatura, predadores e parasitóides, dossel e idade das plantas, tipos e densidades de tricomas foliares, compostos químicos foliares, níveis de N e de K foliares, sobre a intensidade de ataque de mosca-branca Bemisia tabaci (Hemiptera: Aleyrodidae em jiloeiro Solanum gilo var. 'Gigante Portuguesa'. As densidades de ninfas e de adultos de B. tabaci foram maiores nas folhas baixeiras do que nas apicais das plantas de jiloeiro. Com o aumento da temperatura do ar, observou-se incremento na densidade de adultos de B. tabaci nas folhas de jiloeiro.The objective of this study was to determine the effect of rainfall and temperature, predators and parasitoids, height within the canopy and plant age, chemical composition of leaves, levels of nitrogen and potassium in leaves and density of trichomes in leaves, on attack intensity of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae on Solanum gilo var. 'Portuguese giant'. Densities of nymphs and of adults of B. tabaci were higher on leaves of the lower part than on those of the apical parts of plants of S. gilo. The number of adults of B. tabaci on leaves of S. gilo increased with temperature.

  18. Members of Bemisia tabaci (Hemiptera: Aleyrodidae) Cryptic Species and the Status of Two Invasive Alien Species in the Yunnan Province (China)

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    Hu, Jian; Jiang, Zhi-Lin; Nardi, Francesco; Liu, Yuan-Yuan; Luo, Xiao-Rong; Li, Hong-Xiang; Zhang, Zhong-Kai

    2014-01-01

    Abstract Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cryptic species complex that includes some of the most significant pests of agriculture and horticulture worldwide. To understand the diversity and distribution of B. tabaci cryptic species in Yunnan, a famous biodiversity hotspot in China, a large-scale sampling was conducted from year 2010 to 2013 in 10 prefectures. Mitochondrial cytochrome oxidase I gene sequences were used to identify different cryptic species. Phylogenetic analyses were performed using Bayesian methods to assess the position of a new B. tabaci cryptic species in the context of the B. tabaci diversity in Asia. The survey indicates at least eight B. tabaci cryptic species are present in Yunnan, two invasive (MEAM1 and MED) and six indigenous (China 2, China3, China 4, Asia I, Asia II 1, and Asia II 6), MEAM1, MED, and Asia I being the three predominant cryptic species in Yunnan. Compared with MEAM1, MED has a wider distribution. Based on molecular data, a new cryptic species, here named China 4, was identified that appears to be related to China 1, China 2, and China 3. Future efforts should focus on the interactions between predominant B. tabaci cryptic species and begomoviruses and on the development of effective control strategies. PMID:25502045

  19. How to Start with a Clean Crop: Biopesticide Dips Reduce Populations of Bemisia tabaci (Hemiptera: Aleyrodidae on Greenhouse Poinsettia Propagative Cuttings

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    Rosemarije Buitenhuis

    2016-09-01

    Full Text Available (1 Global movement of propagative plant material is a major pathway for introduction of Bemisia tabaci (Hemiptera: Aleyrodidae into poinsettia greenhouses. Starting a poinsettia crop with high pest numbers disrupts otherwise successful biological control programs and widespread resistance of B. tabaci against pesticides is limiting growers’ options to control this pest; (2 This study investigated the use of several biopesticides (mineral oil, insecticidal soap, Beauveria bassiana, Isaria fumosorosea, Steinernema feltiae and combinations of these products as immersion treatments (cutting dips to control B. tabaci on poinsettia cuttings. In addition, phytotoxicity risks of these treatments on poinsettia cuttings, and effects of treatment residues on mortality of commercial whitefly parasitoids (Eretmocerus eremicus and Encarsia formosa were determined; (3 Mineral oil (0.1% v/v and insecticidal soap (0.5% + B. bassiana (1.25 g/L were the most effective treatments; only 31% and 29%, respectively, of the treated B. tabaci survived on infested poinsettia cuttings and B. tabaci populations were lowest in these treatments after eight weeks. Phytotoxicity risks of these treatments were acceptable, and dip residues had little effect on survival of either parasitoid, and are considered highly compatible; (4 Use of poinsettia cutting dips will allow growers to knock-down B. tabaci populations to a point where they can be managed successfully thereafter with existing biocontrol strategies.

  20. Analysis of Species, Subgroups, and Endosymbionts of Bemisia tabaci (Hemiptera: Aleyrodidae) From Southwestern Cotton Fields in Turkey.

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    Karut, Kamil; Mete Karaca, M; Döker, Ismail; Kazak, Cengiz

    2017-08-01

    Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is one of the most important insect pests worldwide including Turkey. Although there are substantial data regarding species composition of Turkish B. tabaci populations, the situation is still not clear and further investigations are needed. Therefore, in this study, species and subgroups of B. tabaci collected from cotton fields in southwestern part of Turkey (Antalya, Aydın, Denizli, and Muğla) were determined using microsatellite analysis, AluI-based mtCOI polymerase chain reaction-random length polymorphism, and sequencing. Secondary endosymbionts were also determined using diagnostic species-specific PCR. Middle East Asia Minor 1 (MEAM1), Mediterranean (MED) Q1, and MED Q2 were the species and subgroups found in this study. The MED species (85.3%) were found to be more dominant than MEAM1. Species status of B. tabaci varied depending on the location. Although all samples collected from Aydın were found to be Q1, three species and subgroups were found in Muğla. Secondary endosymbionts varied according to species and subgroups. Arsenophonus was found only from Q2, while Hamiltonella was detected in MEAM1 and Q1. In addition, high Rickettsia and low Wolbachia infections were detected in MEAM1 and Q1 populations, respectively. In conclusion, for the first time, we report the presence and symbiotic communities of Q1 from Turkey. We also found that the symbiont complement of the Q1 is more congruent with Q1 from Greece than other regions of the world, which may have some interesting implications for movement of this invasive subgroup. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  1. Application of RNA-seq for mitogenome reconstruction, and reconsideration of long-branch artifacts in Hemiptera phylogeny

    Science.gov (United States)

    Song, Nan; An, Shiheng; Yin, Xinming; Cai, Wanzhi; Li, Hu

    2016-01-01

    Hemiptera make up the largest nonholometabolan insect assemblage. Despite previous efforts to elucidate phylogeny within this group, relationships among the major sub-lineages remain uncertain. In particular, mitochondrial genome (mitogenome) data are still sparse for many important hemipteran insect groups. Recent mitogenomic analyses of Hemiptera have usually included no more than 50 species, with conflicting hypotheses presented. Here, we determined the nearly complete nucleotide sequence of the mitogenome for the aphid species of Rhopalosiphum padi using RNA-seq plus gap filling. The 15,205 bp mitogenome included all mitochondrial genes except for trnF. The mitogenome organization and size for R. padi are similar to previously reported aphid species. In addition, the phylogenetic relationships for Hemiptera were examined using a mitogenomic dataset which included sequences from 103 ingroup species and 19 outgroup species. Our results showed that the seven species representing the Aleyrodidae exhibit extremely long branches, and always cluster with long-branched outgroups. This lead to the failure of recovering a monophyletic Hemiptera in most analyses. The data treatment of Degen-coding for protein-coding genes and the site-heterogeneous CAT model show improved suppression of the long-branch effect. Under these conditions, the Sternorrhyncha was often recovered as the most basal clade in Hemiptera. PMID:27633117

  2. Sensitivity of Bemisia Tabaci (Hemiptera: Aleyrodidae) to Several New Insecticides in China: Effects of Insecticide Type and Whitefly Species, Strain, and Stage

    Science.gov (United States)

    Xie, Wen; Liu, Yang; Wang, Shaoli; Wu, Qingjun; Pan, Huipeng; Yang, Xin; Guo, Litao; Zhang, Youjun

    2014-01-01

    Abstract Whitefly biotypes B and Q are the two most damaging members of the Bemisia tabaci (Hemiptera: Aleyrodidae) species complex. Control of B. tabaci (and especially of Q) has been impaired by resistance to commonly used insecticides. To find new insecticides for B. tabaci management in China, we investigated the sensitivity of eggs, larvae, and adults of laboratory strains of B and Q (named Lab-B and Lab-Q) and field strains of Q to several insecticides. For eggs, larvae, and adults of B. tabaci and for six insecticides (cyantraniliprole, chlorantraniliprole, pyriproxyfen, buprofezin, acetamiprid, and thiamethoxam), LC 50 values were higher for Lab-Q than for Lab-B; avermectin LC 50 values, however, were low for adults of both Lab-Q and Lab-B. Based on the laboratory results, insecticides were selected to test against eggs, larvae, and adults of four field strains of B. tabaci Q. Although the field strains differed in their sensitivity to the insecticides, the eggs and larvae of all strains were highly sensitive to cyantraniliprole, and the adults of all strains were highly sensitive to avermectin. The eggs, larvae, and adults of B. tabaci Q were generally more resistant than those of B. tabaci B to the tested insecticides. B. tabaci Q eggs and larvae were sensitive to cyantraniliprole and pyriproxyfen, whereas B. tabaci Q adults were sensitive to avermectin. Field trials should be conducted with cyantraniliprole, pyriproxyfen, and avermectin for control of B. tabaci Q and B in China. PMID:25434040

  3. Leaf Morphological Characters Can Be a Factor for Intra-Varietal Preference of Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae among Eggplant Varieties.

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    Abu Tayeb Mohammad Hasanuzzaman

    Full Text Available The sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae MEAM1, is considered a serious pest of horticultural and many other crops. While eggplant (Solanum melongena is one of the most favored host plants, the whiteflies exhibit preferences among different varieties. We hypothesized that certain morphological leaf characteristics of different varieties, like leaf trichome density, trichome length, leaf lamina thickness and leaf color, may affect whitefly landing, feeding and oviposition. In this study, we investigated the variation in leaf morphological characters among selected eggplant varieties and evaluated the effect of these leaf characteristics in rendering eggplant varieties either susceptible or resistant to B. tabaci. We evaluated eight eggplant varieties in choice feeding tests, and we found that the varieties JinSheng Zilongchangqie (JSZ and H149 were the highly preferred varieties with the highest numbers of whitefly adults and eggs. Significantly lower numbers of whitefly adult eggs were found on the resistant variety Tuo Lu Bamu (TLB. The varieties JinGuangbo Luqie (JGL, JinGuangbo Ziquanqie (JGZ, DaYang Ziguanqie (DYZ, QinXing Ziguanqie (QXZ, and QinXing Niuxinqie (QXN were moderately favored by B. tabaci. Leaf trichome density, trichome length and leaf lamina thickness were positively correlated with numbers of whitefly adults and eggs. B. tabaci was less attracted to the leaves that reflect long and middle wavelength light (higher R and G values than to the bright green leaves (medium G value, but the short wavelength light (higher B value had no significant effect on whitefly preference. The degree of hue had a positive effect, and saturation and brightness had a negative effect on whitefly attraction.

  4. Pathogenicity of Two Species of Entomopathogenic Nematodes Against the Greenhouse Whitefly, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae), in Laboratory and Greenhouse Experiments.

    Science.gov (United States)

    Rezaei, Nastaran; Karimi, Javad; Hosseini, Mojtaba; Goldani, Morteza; Campos-Herrera, Raquel

    2015-03-01

    The greenhouse whitefly Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) is a polyphagous pest in greenhouse crops. The efficacy of two entomopathogenic nematodes (EPN), Steinernema feltiae and Heterorhabditis bacteriophora, as biological control agents against T. vaporariorum was evaluated using two model crops typical of vegetable greenhouse productions: cucumber and pepper. Laboratory tests evaluated adults and second nymphal instars for pest susceptibility to different EPN species at different concentrations of infective juveniles (IJ; 0, 25, 50, 100, 150, 200, and 250 IJ per cm(2)); subsequent greenhouse trials against second nymphal instars on cucumber and pepper plants evaluated more natural conditions. Concentrations were applied in combination with Triton X-100 (0.1% v/v), an adjuvant for increasing nematode activity. In laboratory studies, both life stages were susceptible to infection by the two nematode species, but S. feltiae recorded a lower LC50 than H. bacteriophora for both insect stages. Similarly, in greenhouse experiments, S. feltiae required lower concentrations of IJ than H. bacteriophora to reach the same mortality in nymphs. In greenhouse trials, a significant difference was observed in the triple interaction among nematode species × concentration × plant. Furthermore, the highest mortality rate of the second nymphal instars of the T. vaporariorum was obtained from the application of S. feltiae concentrated to 250 IJ/cm(2) on cucumber (49 ± 1.23%). The general mortality caused by nematodes was significantly higher in cucumber than in pepper. These promising results support further investigation for the optimization of the best EPN species/concentration in combination with insecticides or adjuvants to reach a profitable control of this greenhouse pest.

  5. Thermotolerance and Heat-Shock Protein Gene Expression Patterns in Bemisia tabaci (Hemiptera: Aleyrodidae) Mediterranean in Relation to Developmental Stage.

    Science.gov (United States)

    Jiang, Rui; Qi, Lan-Da; Du, Yu-Zhou; Li, Yuan-Xi

    2017-10-01

    Temperature plays an important role in the growth, development, and geographic distribution of insects. There is convincing evidence that heat-shock proteins (HSPs) play important roles in helping organisms adapt to thermal stress. To better understand the physiological and ecological influence of thermal stress on the different development stages of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Mediterranean species (MED), nymphs and adults were shocked with temperatures of 35, 38, and 41℃ for 1 and 2 h, respectively, and the survival rate, fecundity, and developmental duration were investigated in the laboratory. The expression levels of the hsp40, hsp70, and hsp90 genes were assessed using real-time PCR. The results indicate that the survival rates of the nymphs and adults decreased with increased temperature. A 2-h heat shock at 41℃ induced a significant reduction in fecundity in adults and an increase in developmental duration in young nymphs. Hsp90 showed higher temperature responses to thermal stress than hsp40 or hsp70. The expression levels of the hsps in the adults were significantly down-regulated by a 2-h heat shock at 41℃ compared with that by a 1-h treatment. A significant decrease in the expression levels of the hsps also occurred in the adults when the temperature increased from 38 to 41℃ for the 2-h treatment, whereas no significant decrease occurred in the nymphs. Compared with previous studies, we provide some evidence indicating that MED has the potential to adapt to a wider temperature range than the Middle East-Asia Minor 1 species. © The Author 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  6. Sensitivity of Bemisia tabaci (Hemiptera: Aleyrodidae) to several new insecticides in China: effects of insecticide type and whitefly species, strain, and stage.

    Science.gov (United States)

    Xie, Wen; Liu, Yang; Wang, Shaoli; Wu, Qingjun; Pan, Huipeng; Yang, Xin; Guo, Litao; Zhang, Youjun

    2014-01-01

    Whitefly biotypes B and Q are the two most damaging members of the Bemisia tabaci (Hemiptera: Aleyrodidae) species complex. Control of B. tabaci (and especially of Q) has been impaired by resistance to commonly used insecticides. To find new insecticides for B. tabaci management in China, we investigated the sensitivity of eggs, larvae, and adults of laboratory strains of B and Q (named Lab-B and Lab-Q) and field strains of Q to several insecticides. For eggs, larvae, and adults of B. tabaci and for six insecticides (cyantraniliprole, chlorantraniliprole, pyriproxyfen, buprofezin, acetamiprid, and thiamethoxam), LC50 values were higher for Lab-Q than for Lab-B; avermectin LC50 values, however, were low for adults of both Lab-Q and Lab-B. Based on the laboratory results, insecticides were selected to test against eggs, larvae, and adults of four field strains of B. tabaci Q. Although the field strains differed in their sensitivity to the insecticides, the eggs and larvae of all strains were highly sensitive to cyantraniliprole, and the adults of all strains were highly sensitive to avermectin. The eggs, larvae, and adults of B. tabaci Q were generally more resistant than those of B. tabaci B to the tested insecticides. B. tabaci Q eggs and larvae were sensitive to cyantraniliprole and pyriproxyfen, whereas B. tabaci Q adults were sensitive to avermectin. Field trials should be conducted with cyantraniliprole, pyriproxyfen, and avermectin for control of B. tabaci Q and B in China. © The Author 2014. Published by Oxford University Press on behalf of the Entomological Society of America.

  7. Assessing Compatibility of Isaria fumosorosea and Buprofezin for Mitigation of Aleurodicus rugioperculatus (Hemiptera: Aleyrodidae): An Invasive Pest in the Florida Landscape.

    Science.gov (United States)

    Kumar, Vivek; Francis, Antonio; Avery, Pasco B; McKenzie, Cindy L; Osborne, Lance S

    2018-05-28

    Rugose spiraling whitefly (RSW), Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) is a new invasive whitefly pest in the Florida landscape, known to feed on a wide range of plants including palms, woody ornamentals, shrubs, and fruits. With the objective to find an alternative to neonicotinoid insecticides, and develop an ecofriendly management program for RSW, in the current study we evaluated the efficacy of a biopesticide containing the entomopathogenic fungi, Isaria fumosorosea Wize (Hypocreales: Clavicipitaceae), and an insect growth regulator buprofezin applied alone and in combination under laboratory and field conditions. Before assessing the two products, their compatibility was studied at six different concentrations of buprofezin. No significant inhibitive effect of buprofezin was observed on I. fumosorosea spore germination, and the average linear growth of the colony measured 14 d postinoculation. Under laboratory conditions, I. fumosorosea treatments (alone or mixed with buprofezin) provided higher RSW mortality than buprofezin alone. However, in both outdoor cage studies, the efficacy of buprofezin treatments (alone or mixed with I. fumosorosea) was higher than I. fumosorosea alone. A significant reduction in RSW population was reported for more than 5 wk in buprofezin alone and more than 7 wk in the combination treatments. In fall of 2014 and summer of 2015, the mean whitefly mortality observed during the 10-wk assessment period was 52.4 and 42.1% for I. fumosorosea, 79.6 and 79.0% for buprofezin, and 87.6 and 84.3% in mixed treatments, respectively. Results suggest that buprofezin can offer an effective alternate in the battle against invasive whiteflies such as RSW in Florida ecosystems, either as a stand-alone strategy or in an integrated approach.

  8. Susceptibility of MED-Q1 and MED-Q3 Biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) Populations to Essential and Seed Oils.

    Science.gov (United States)

    Samuel Fogné, Drabo; Olivier, Gnankine; Bassolé, Imael H N; Nébié, Roger Charles; Laurence, Mouton

    2017-06-01

    Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a major pest of many agricultural and ornamental crops in tropical and subtropical regions causing damages that result in important economic losses. Insecticides are commonly used in greenhouses or fields to control B. tabaci populations leading to rapid evolution of resistance that render treatments inefficient. Therefore, and for environmental and human health concerns, other approaches must be developed for this pest management. In the present study, we compare, using the leaf dip method, the toxicity of three essential oils (Cymbopogon citratus, Ocimum americanum, and Hyptis spicigera) and three seed oils (Lannea microcarpa, Lannea acida, and Carapa procera) with three chemical insecticides (acetamiprid, deltamethrin, and chlorpyrifos-ethyl) on adults. Two B. tabaci biotypes (MED-Q1 and MED-Q3) belonging to the Mediterranean species and collected in Burkina Faso were used. Essential oils were analyzed by gas chromatography-mass spectrometry and gas chromatography-flame ionization detector. We showed that these two biotypes have different levels of resistance to the three insecticides, MED-Q3 being more sensitive than MED-Q1. Moreover, they differ in the frequency of resistance alleles to insecticides, especially for organophosphates, as these alleles are almost fixed in MED-Q1. On the other hand, the two biotypes prove to be more susceptible to the plant extracts than to insecticides except for chlorpyrifos-ethyl, with essential oils that showed the highest insecticidal activities. Monoterpenes content were the most abundant and showed the highest insecticidal activities. Our results indicated that essential oils, but also seed oils, have the potential to constitute an alternative strategy of pest management. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  9. The Effects of Some Botanical Insecticides and Pymetrozine on Life Table Parameters of Silver Leaf Whitefly Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae

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    Reihaneh Barati

    2013-01-01

    Full Text Available The objective of this research was to assess the effects of extracts of two medicinalplant species: Allium sativum (Linn and Calotropis procera (Aiton, and a formulation containingazadirachtin on life table parameters of silver leaf whitefly (SLW, Bemisia tabaci biotypeB (Gennadius (Hemiptera: Aleyrodidae, grown on greenhouse tomato plants. Theeffects were compared to that of pymetrozine, a synthetic insecticide. Bioassays were carriedout in a greenhouse under controlled conditions of 27 ± 2°C, R.H. of 55 ± 5% and 16:8h (L:D photo period.All treatments significantly affected the survivorship and fertility of SLW female adults,reducing the net reproduction rate, mean generation time and intrinsic rate of increase ofthis insect.The net reproductive rate [R0] values for the populations treated with garlic extract,milkweed extract, pymetrozine, azadirachtin, control for extracts (ethanol + distilled waterand control for pesticides (distilled water were 23.58, 19.32, 10.78, 8.23, 49.66, 57.55; theintrinsic rate of increases [rm] were 0.134, 0.139, 0.110, 0.090, 0.177, 0.178; the mean generationtimes [T] were 23.49, 21.23, 21.66, 23.50, 22.06, 22.69; the doubling times [DT] were 5.14,4.95, 6.27, 7.56, 3.91, 3.87, and the finite rates of increase [λ] were 1.144, 1.149, 1.116, 1.094, 1.193,1.195, respectively. Azadirachtin had the highest effect on the life table parameters of SLW.Our findings indicated that, although herbal extracts were not effective as much asthe chemical insecticides, they can be effective in pest control. Therefore, they are suitablechoices for replacing chemical insecticides and for alternative use with azadirachtin inSLW IPM program.

  10. Sublethal effects of some synthetic and botanical insecticides on Bemisia tabaci (Hemiptera: Aleyrodidae

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    Esmaeily Saeideh

    2014-07-01

    Full Text Available In addition to direct mortality caused by insecticides, some biological traits of insects may also be affected by sublethal insecticide doses. In this study, we used the age-stage, two-sex life table method to evaluate the sublethal effects of the four synthetic insecticides: abamectin, imidacloprid, diazinon, and pymetrozin as well as the botanical insecticide taken from Calotropis procera (Asclepiadaceae extract, on eggs of the cotton whitefly, Bemisia tabaci (Hem.: Aleyrodidae. The lowest and highest survival rates and oviposition periods were observed in whiteflies treated by diazinon and imidacloprid, respectively. We found significant differences in the net reproductive rate (R0, the intrinsic rate of increase (r, the finite rate of increase (?, and the gross reproductive rate (GRR among different insecticides. Altogether, our results showed that pymetrozin and C. procera induced the most sublethal effects, thus they may be suitable candidates for use in integrated pest management programs of B. tabaci.

  11. Primeiro Registro de Afídeos (Hemiptera: Aphidoidea) em Carambola (Averrhoa carambola L.) no Brasil

    OpenAIRE

    Cunha, Suzan Beatriz Zambon da; Sousa e Silva, Carlos Roberto

    2016-01-01

    A carambola (Averrhoa carambola L.) árvore de origem Asiática foi introduzida no Brasil em 1817, desde então vem aumentando sua área de cultivo no país. A planta é atacada por poucos insetos como as moscas e percevejos. Este trabalho relata a presença de Aphis gossypii Glover em A. carambola pela primeira vez no Brasil. First Record of Aphids (Hemiptera: Aphididae) on Starfruit (Averrhoa carambola L.) in Brazil Abstract. The starfruit (Averrhoa carambola L.) has origin Asiatic an...

  12. Whitefly population dynamics in okra plantations Dinâmica populacional de mosca-branca em quiabo

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    Germano Leão Demolin Leite

    2005-01-01

    Full Text Available The control of whitefly Bemisia tabaci (Gennadius biotype B (Hemiptera: Aleyrodidae on okra (Abelmoschus esculentus L. consists primarily in the use of insecticides, due to the lack of information on other mortality factors. The objective of this study was to evaluate the spatial and temporal population dynamics of the whitefly B. tabaci biotype B on two successive A. esculentus var. "Santa Cruz" plantations. Leaf chemical composition, leaf nitrogen and potassium contents, trichome density, canopy height, plant age, predators, parasitoids, total rainfall and median temperature were evaluated and their relationships with whitefly on okra were determined. Monthly number estimates of whitefly adults, nymphs (visual inspection and eggs (magnifying lens occurred on bottom, middle and apical parts of 30 plants/plantation (one leaf/plant. Plants senescence and natural enemies, mainly Encarsia sp., Chrysoperla spp. and Coccinellidae, were some of the factors that most contributed to whitefly reduction. The second okra plantation, 50 m apart from the first, was strongly attacked by whitefly, probably because of the insect migration from the first to the second plantation. No significant effects of the plant canopy on whitefly eggs and adults distribution were found. A higher number of whitefly nymphs was found on the medium part than on the bottom part.O controle da mosca-branca Bemisia tabaci (Gennadius biótipo B (Hemiptera: Aleyrodidae em quiabeiro (Abelmoschus esculentus L. consiste principalmente no uso de inseticidas, em virtude da falta de informação sobre outros fatores de mortalidade. O objetivo deste trabalho foi compreender a dinâmica populacional, espacial e temporal da mosca-branca em dois cultivos sucessivos de quiabeiro "Santa Cruz". Avaliaram-se a composição química foliar, os níveis foliares de nitrogênio e de potássio, a densidade de tricomas, a altura de dossel, a idade de planta, predadores, parasitóides, pluviosidade total

  13. Primeiro registro de ocorrência do parasitóide Brasema sp. (hymenoptera: eupelmidae em ovos de Leptoglossus zonatus (Dallas, 1852 (hemiptera: coreidae no Brasil First record of occurrence of the parasitoid Brasema sp. (hymenoptera: eupelmidae in eggs of Leptoglossus zonatus (Dallas, 1852 (hemiptera: coreidae in Brazil

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    Carlos Henrique Marchiori

    2002-12-01

    Full Text Available Este trabalho registra a primeira ocorrência do parasitóide Brasema sp. (Hymenoptera: Eupelmidae parasitando ovos de Leptoglossus zonatus (Hemiptera: Coreidae em cultivar de milho em Itumbiara, GO. A porcentagem de parasitismo foi de 4,8%. Brasema sp. constitue-se em ectoparasitóide de larvas de coleópteros e de outros hospedeiros no interior de tecidos de plantas.This work reports, for the first time, of parasitoid Brasema sp. (Hymenoptera: Eupelmidae parasitizinf eggs of Leptoglossus zonatus (Hemiptera: Coreidae in maize cultivate in Itumbiara, GO, Brazil. The percentage of parasitizing was 4,8%. Brasema sp. constitutes ectoparasitoid of beetle larvae and other hosts concealed in plant tissue.

  14. CASOS SELECTOS DE RESISTENCIA A INSECTICIDAS EN MOSCAS BLANCAS (Hemiptera: Aleyrodidae EN EL MUNDO

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    Macías-flores A

    2013-01-01

    Full Text Available Las moscas blancas (Hemíptera: Aleyrodidae son un problema a escala global. En México, llamó la atención como plaga agrícola en la década de los años 80´s y 90´s del siglo pasado por las pérdidas económicas que ocasionaron en los cultivos agrícolas, debido a los daños directos por la succión de savia y a los daños indirectos por la transmisión de enfermedades de origen viral. El hombre en su intento fallido por erradicarlas ha hecho uso desmedido de insecticidas químicos, quizás por su accesibilidad por costo bajo o por efectos de control inmediatos, por lo cual ha causado daño al medio ambiente y a la salud humana, con la consecuencia de desarrollo de resistencia. En un intento por disminuir los efectos de tales sustancias, muchas investigaciones alrededor del mundo se han conducido para encontrar productos insecticidas más efectivos con el menor impacto ambiental y a la salud humana, y que contribuyan a incrementar las herramientas de control disponibles en los sistemas de producción agrícola.

  15. Genetic variation of the greenhouse whitefly, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae), among populations from Serbia and neighbouring countries, as inferred from COI sequence variability.

    Science.gov (United States)

    Prijović, M; Skaljac, M; Drobnjaković, T; Zanić, K; Perić, P; Marčić, D; Puizina, J

    2014-06-01

    The greenhouse whitefly Trialeurodes vaporariorum Westwood, 1856 (Hemiptera: Aleyrodidae) is an invasive and highly polyphagous phloem-feeding pest of vegetables and ornamentals. Trialeurodes vaporariorum causes serious damage due to direct feeding and transmits several important plant viruses. Excessive use of insecticides has resulted in significantly reduced levels of susceptibility of various T. vaporariorum populations. To determine the genetic variability within and among populations of T. vaporariorum from Serbia and to explore their genetic relatedness with other T. vaporariorum populations, we analysed the mitochondrial cytochrome c oxidase I (COI) sequences of 16 populations from Serbia and six neighbouring countries: Montenegro (three populations), Macedonia (one population) and Croatia (two populations), for a total of 198 analysed specimens. A low overall level of sequence divergence and only five variable nucleotides and six haplotypes were found. The most frequent haplotype, H1, was identified in all Serbian populations and in all specimens from distant localities in Croatia and Macedonia. The COI sequence data that was retrieved from GenBank and the data from our study indicated that H1 is the most globally widespread T. vaporariorum haplotype. A lack of spatial genetic structure among the studied T. vaporariorum populations, as well as two demographic tests that we performed (Tajima's D value and Fu's Fs statistics), indicate a recent colonisation event and population growth. Phylogenetic analyses of the COI haplotypes in this study and other T. vaporariorum haplotypes that were retrieved from GenBank were performed using Bayesian inference and median-joining (MJ) network analysis. Two major haplogroups with only a single unique nucleotide difference were found: haplogroup 1 (containing the five Serbian haplotypes and those previously identified in India, China, the Netherlands, the United Kingdom, Morocco, Reunion and the USA) and haplogroup 3

  16. First report and morphological redescription of Teleonemia morio (Stål (Hemiptera, Tingidae in Annona squamosa L. (Annonaceae in Brazil

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    Sônia M. F. Broglio

    2012-03-01

    Full Text Available First report and morphological redescription of Teleonemia morio (Stål (Hemiptera, Tingidae in Annona squamosa L. (Annonaceae in Brazil. This is the first report of a severe attack of Teleonemia morio (Stål, 1855 (Hemiptera, Heteroptera, Tingidae on Annona squamosa L. (custard apple, causing up to 80% of losses of infested trees. In order to facilitate the identification of this insect pest, the adult female of T. morio is redescribed based on specimens collected in Palmeira dos Índios, Alagoas, Brazil.Primeiro registro e redescrição morfológica de Teleonemia morio (Stål (Hemiptera, Tingidae em Annona squamosa L. (Annonaceae no Brazil. Este é o primeiro registro de um ataque severo de Teleonemia morio (Stål, 1855 (Hemiptera, Heteroptera, Tingidae em árvores de Annona squamosa L. (pinheira ou fruta-do-conde, resultando em perdas de aproximadamente 80% das plantas infestadas. Com o objetivo de facilitar a identificação deste inseto-praga, foi feita a redescrição da fêmea adulta de T. morio com base em espécimes coletados em Palmeira dos Índios, Alagoas, Brasil.

  17. Compatibility of the insect pathogenic fungus Beauveria bassiana with neem against sweetpotato whitefly, Bemisia tabaci, on eggplant

    Science.gov (United States)

    A study on the compatibility of the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin (Ascomycota: Hypocreales) with neem was conducted against sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), on eggplant. Initially, three concentrations of B. bassiana (106, 1...

  18. Biology of Maconellicoccus hirsutus (Hemiptera: Pseudococcidae on Hibiscus rosa-sinensis = Biologia de Maconellicoccus hirsutus (Hemiptera: Pseudococcidae em Hibiscus rosa-sinensis

    Directory of Open Access Journals (Sweden)

    Marcelo Negrini

    2017-10-01

    Full Text Available Maconellicoccus hirsutus Green, 1908 (Hemiptera: Pseudococcidae is an introduced pest that threatens fruit and ornamental plant production in Brazil. Reported in Brazil for the first time in 2010, in the state of Roraima, M. hirsutus, has spread rapidly to other regions of the country. The objective of this study was to investigate the biology of M. hirsutus on Hibiscus rosa-sinensis L. (Malvaceae leaves in laboratory conditions with climatic parameters similar to those of the Brazilian North and Northeast (27 ± 1 °C, 60 ± 10% relative humidity and 12 hours light and 12 hours dark. Eighty M. hirsutus nymphs were individualized on rooted H. rosa-sinensis leaves and their survival, mortality, sex ratio, and egg numbers per female emerged were recorded. Maconellicoccus hirsutus can have up to nine generations per year, taking 6.5 days to double its population size. The female numbers of this pest are about three times higher than those for its male counterparts, with 98 eggs per female and 97% viability. The survival curve of the species is type I, that is, mortality rates are higher in adulthood. Maconellicoccus hirsutus, especially, a pest of fruit trees and ornamental plants, presents great potential for population growth in Brazilian tropical conditions. = Maconellicoccus hirsutus Green, 1908 (Hemiptera: Pseudococcidae é uma praga introduzida que ameaça a produção de frutas e plantas ornamentais no Brasil. Registrada no Brasil pela primeira vez em 2010, no estado de Roraima, M. hirsutus se espalhou rapidamente para outras regiões do país. O objetivo deste estudo foi investigar a biologia de M. hirsutus em folhas de Hibiscus rosa-sinensis L. (Malvaceae, em condições de laboratório com parâmetros climáticos semelhantes aos das regiões Norte e Nordeste do Brasil (27 ± 1 °C, 60 ± 10 % de umidade relativa e 12 h de luz e 12 h de escuro. Oitenta ninfas de M. hirsutus foram individualizadas em folhas enraizadas de H. rosa-sinensis e

  19. Registro de Thaumastocoris peregrinus (Hemiptera, Thaumastocoridae) no Estado do Paraná

    OpenAIRE

    Barbosa, Leonardo Rodrigues; Santos, Franciele; Wilcken, Carlos F.; Soliman, Everton P.

    2010-01-01

    It is recorded the occurrence of Thaumastocoris peregrinus Carpintero & Dellapé, 2006 (Hemiptera, Thaumastocoridae) in eucalyptus trees in the State of Parana, Brazil. The insect was observed in June at 2009, in Curitiba region.

    doi: 10.4336/2010.pfb.30.61.75

    É registrada a ocorrência de Thaumastocoris peregrinus Carpintero & Dellapé, 2006 (Hemiptera, Thaumastocoridae) em árvores de eucalipto no Estado do Paraná. Infestações foram o...

  20. REPRODUÇÃO DE Edessa meditabunda (HEMIPTERA: PENTATOMIDAE EM ALGODOEIRO

    Directory of Open Access Journals (Sweden)

    Rosalia Azambuja

    2015-05-01

    Full Text Available O percevejo-asa-preta-da-soja Edessa meditabunda (Fabricius (Hemiptera: Pentatomidae no final do ciclo da soja dispersa para algodoeiro e utiliza essa planta como hospedeiro alternativo. Visando conhecer o potencial desta espécie como praga em algodoais e auxiliar na tomada decisão quanto ao manejo deste inseto, hipotetizou-se nesse trabalho que o algodoeiro é nutricionalmente adequado para a reprodução e desenvolvimento de E. meditabunda. Os tratamentos testados foram: 1 folhas e maçã de algodoeiro e 2 dieta padrão, recomendada para criação de pentatomídeos fitófagos em laboratório, utilizada como testemunha. As características biológicas avaliadas foram: período de desenvolvimento ninfal, duração de cada ínstar, porcentagem de sobrevivência, peso dos adultos na emergência, longevidade de machos e de fêmeas, período de pré-oviposição e de oviposição, número total de ovos por fêmea e fecundidade das fêmeas. Observou-se que, apesar do prolongamento do período de desenvolvimento ninfal, as ninfas alimentadas com algodoeiro sobreviveram, atingiram a fase adulta e os adultos se reproduziram, o que nos permite sugerir que o algodoeiro seja uma planta nutricionalmente adequada para o desenvolvimento e reprodução de E. meditabunda, permitindo a manutenção do inseto em campo após a colheita da soja.  RESUMO El chinche Edessa meditabunda (Fabricius (Hemiptera: Pentatomidae al final del ciclo de la soja emigra al cultivo de algodón y utiliza esta planta como un huésped alternativo. Con el objetivo de conocer el potencial de esta especie como una plaga en los algodonales y ayudar en la toma de decisiones con respecto al manejo de este insecto, se planteó la hipótesis en este estudio que el algodón y nutritiva, adecuada para la reproducción y el desarrollo de E. meditabunda. Los tratamientos fueron:1 las hojas y el fruto del algodón 2 dieta estándar recomendada para la cría de pentatomidos fitófagos en el

  1. Atividade de buprofezin sobre a cigarrinha verde do feijoeiro Empoasca kraemeri (Ross & Moore, 1957) (Hemiptera, Cicadellidae) em condições de laboratório

    OpenAIRE

    Moreno,Paulo Rogério; Nakano,Octávio

    2002-01-01

    A espécie Empoasca kraemeri é uma importante praga para a cultura do feijoeiro (Phaseolus vulgaris L.) em toda a América Latina, sendo necessário recorrer ao controle químico quando a praga atinge alta população. Neste trabalho, foi estudado o efeito do inseticida buprofezin sobre ninfas de primeiro instar de Empoasca kraemeri (Hemiptera, Cicadellidae), sob condições de laboratório. Esta fase da praga mostrou-se altamente susceptível ao inseticida, sendo a CL50 estimada de 0,112 mg L-1. Em ou...

  2. Diversity and Distribution of Cryptic Species of the Bemisia tabaci (Hemiptera: Aleyrodidae) complex in Pakistan.

    Science.gov (United States)

    Masood, Mariyam; Amin, Imran; Hassan, Ishtiaq; Mansoor, Shahid; Brown, Judith K; Briddon, Rob W

    2017-12-05

    Bemisia tabaci (Gennadius; Hempitera: Aleyrodidae) is considered to be a cryptic (sibling) species complex, the members of which exhibit morphological invariability while being genetically and behaviorally distinct. Members of the complex are agricultural pests that cause direct damage by feeding on plants, and indirectly by transmitting viruses that cause diseases leading to reduced crop yield and quality. In Pakistan, cotton leaf curl disease, caused by multiple begomovirus species, is the most economically important viral disease of cotton. In the study outlined here, the diversity and geographic distribution of B. tabaci cryptic species was investigated by analyzing a taxonomically informative fragment of the mitochondrial cytochrome c oxidase 1 gene (mtCOI-3'). The mtCOI-3' sequence was determined for 285 adult whiteflies and found to represent six cryptic species, the most numerous being Asia II-1 and Middle East Asia Minor 1 (MEAM-1), the later also referred to as the B-biotype, which was previously thought to be confined to Sindh province but herein, was also found to be present in the Punjab province. The endemic Asia I was restricted to Sindh province, while an individual in the Asia II-8 was identified in Pakistan for the first time. Also for the first time, samples were collected from northwestern Pakistan and Asia II-1 was identified. Results indicate that in Pakistan the overall diversity of B. tabaci cryptic species is high and, based on comparisons with findings from previous studies, the distribution is dynamic. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  3. Sources of wild germplasm to improve whitefly resistance in commercial watermelon (Citrullus lanatus var. lanatus)

    Science.gov (United States)

    Host plant resistance is a fundamental component of crop sustainability. The Bemisia tabaci (Hemiptera: Aleyrodidae) whitefly complex is well known as a key pest of many crops around the world. It is adaptive to its environment and feeds on an impressive number of plant species (over 1,000). Yet, th...

  4. Parâmetros biológicos e tabela de vida de fertilidade de Neopamera bilobata (Hemiptera: Rhyparochromidae em morangueiro

    Directory of Open Access Journals (Sweden)

    Taciana Melissa de Azevedo Kuhn

    2014-06-01

    Full Text Available O objetivo deste trabalho foi avaliar os parâmetros biológicos e determinar a tabela de vida de fertilidade de Neopamera bilobata (Hemiptera: Rhyparochromidae em morangueiro. O experimento foi realizado no Laboratório de Entomologia da Embrapa Uva e Vinho, em Bento Gonçalves, RS, em câmara climatizada (a 23±1°C, 70±10% UR e fotófase de 12 horas. Utilizaram-se folíolos, flores, frutos verdes e maduros de morangueiro 'Aromas' como alimento para os percevejos. O desenvolvimento das fases imaturas e os parâmetros biológicos dos adultos foram monitorados diariamente. O percevejo N. bilobata não completou o ciclo biológico em folíolos e flores de morangueiro. Em frutos maduros e verdes, o tempo de desenvolvimento da ninfa ao adulto foi de 32,8±9,12 e 36,7±6,80 dias, com viabilidade de 27,3 e 51%, respectivamente. O período de oviposição foi de 34,7±21,2 dias, com 319,1±262,7 ovos em frutos maduros, e de 43,9±18,3 dias, com 318,2±144,7 ovos em frutos verdes. Neopamera bilobata completa seu ciclo biológico na cultura do morangueiro. Frutos verdes de morangueiro são mais adequados ao desenvolvimento deste inseto do que frutos maduros.

  5. Caracterización in silico de las proteínas del choque térmico Hsp70 y Hsp90 deBemisia tabaci (Hemiptera: Aleyrodidae y su posible actividad adaptativa

    Directory of Open Access Journals (Sweden)

    Eneida Torres Cabra

    2014-06-01

    Full Text Available La mosca blanca, Bemisia tabaci (Hemiptera: Aleyrodidae es una de las plagas más destructivas e invasivas en el mundo, ataca una gran cantidad de cultivos. Se adapta fácilmente a plantas hospederas y a nuevas regiones geográficas, lo que sugiere el desarrollo de mecanismos de control a daños producidos por factores estresantes. Las proteínas Hsp se expresanen los organismos como mecanismo de defensa, actúan como chaperonas en el correcto ensamblaje de las proteínas. En este estudio se realizó una caracterizaciónin silico de las proteínas Hsp70 y Hsp90 de B. tabaci, secuencias obtenidas de NCBI. La determinaciónde los perfiles de hidrofobicidad, polaridad, accesibilidady flexibilidad se obtuvieron con “ProScale” de ExPASy, el perfil de antigenicidad con JaMBW. La secuencia aminoacídica se analizó con GOR IV y SOPMA y la composición de aminoácidos con ProtParam. Para analizar el peso molecular, índice deinestabilidad, índice alifático y gradiente hidropático,con GRAVY. La estructura terciaria se obtuvo con HHpred, y ESyPred3D. Para validar las estructuras 3D se utilizó Procheck, What_check y errat. Hsp70 y Hsp90 de B. tabaci presentan valores bajos de hidrofobicidady altos de polaridad, flexibilidad y accesibilidad, características que le permiten a las proteínas extender su capacidad como chaperonas. La Hsp70tiene una estructura secundaria compuesta por 41-45% alfa hélices, 30-43% coil y menos del 6% en hoja plegada y la Hsp90 por 52 y 53% hélices, 26-34% coily 6% hoja plegada. Las Hsp juegan un rol importante en los insectos debido a su tamaño y corto ciclo de vida, pues la temperatura influye en su distribución y abundancia.

  6. Efeito de extratos de plantas do Cerrado em Dipetalogaster maxima (Uhler (Hemiptera, Reduviidae Effect of Cerrado plant extracts on Dipetalogaster maxima (Uhler (Hemiptera, Reduviidae

    Directory of Open Access Journals (Sweden)

    André A. M. Coelho

    2009-01-01

    Full Text Available A transmissão da doença de Chagas ocorre, principalmente, por meio de fezes de hemípteros hematófagos (Triatominae, os quais ingerem o Trypanosoma cruzi ao se alimentarem do sangue de pessoas ou outros mamíferos infectados. Para o controle dos triatomíneos, os piretróides são os principais inseticidas utilizados. Entretanto, algumas populações de insetos demonstraram resistência a determinados piretróides, indicando a necessidade do desenvolvimento de novos inseticidas eficazes no controle desses vetores. Assim, foi avaliada a atividade inseticida de 83 extratos vegetais, pertencentes a 35 espécies diferentes, em ninfas do primeiro estádio de Dipetalogaster maxima (Uhler, 1894 (Hemiptera: Reduviidae, triatomíneo encontrado no México. Para o teste tópico, foram aplicados 50 ìg de cada extrato nos tergitos abdominais de dez ninfas, em duplicata. Como controles, foram utilizados insetos tratados com etanol, acetona ou sem nenhum tipo de tratamento. Os triatomíneos foram observados durante 28 dias. Nenhum extrato apresentou atividade inseticida significativa, entretanto, o extrato hexânico do fruto e o etanólico da casca do caule de Simarouba versicolor (Simaroubaceae inibiram a taxa de ecdise em D. maxima (40% e 25%, respectivamente. Sugere-se que estes extratos sejam quimicamente investigados e monitorados por ensaios biológicos a fim de determinar os componentes, para que estes possam ser utilizados como modelos moleculares ou como compostos biorracionais nos programas de controle de insetos.Chagas disease, also known as American Trypanosomiasis, is chiefly transmitted by faeces of haematophagous bugs (Triatominae that ingested Trypanosoma cruzi from blood of infected people or other mammals. Pyrethroids have been the main insecticides used against these insects. However, some populations of insects have shown significant levels of resistance to several pyrethroids, indicating the need of new insecticides for the control of

  7. Occurrence of lace bug Vatiga illudens and Vatiga manihotae (Hemiptera: Tingidae in Mato Grosso do Sul, midwestern Brazil

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    Patrícia P. Bellon

    2012-09-01

    Full Text Available Nymphs and adults of the lace bug (Hemiptera: Tingidae have been found in cassava crops (Manihot esculenta in Mato Grosso do Sul State, Brazil. The insects were collected in the field and taken to the laboratory where they were identified based on some morphological traits of the species Vatiga manihotae (Drake and V. illudens (Drake, which are first reported in the aforementioned state.Ninfas e adultos do percevejo-de-renda (Hemiptera: Tingidae foram encontrados em lavouras de mandioca (Manihot esculenta no Estado do Mato Grosso do Sul, Brasil. Os insetos foram coletados em campo e levados ao laboratório, onde foram identificados com base em algumas características morfológicas nas espécies Vatiga manihotae (Drake e V. illudens (Drake, os quais são registrados pela primeira vez no Estado mencionado.

  8. Ocorrência de Tuthillia cognata Hodkinson, Brown & Burckhardt, 1986(Hemiptera: Homoptera, Psyllidae em plantios experimentais de camu-camu Myrciaria dubia (H.B.K. Mc Vaugh em Manaus (Amazonas, Brasil Occurrence of Tuthillia cognata Hodkinson, Brown & Burckhardt, 1986 (Hemiptera: Homoptera, Psyllidae in experimental plantations of camu-camu Myrciaria dubia (H.B.K. Mc Vaugh in Manaus (Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Márcio Luís Leitão Barbosa

    2004-01-01

    Full Text Available O cultivo de camu-camu Myrciaria dubia (H.B.K. Mc Vaugh tem apresentado inúmeros problemas fitossanitários, dentre os quais, Tuthillia cognata Hodkinson et al. (Hemiptera: Homoptera, Psyllidae, que constantemente é citada como praga secundária. Os objetivos deste estudo foram determinar o nível e a intensidade de infestação (% por T. cognata e estudar aspectos do ciclo biológico e do comportamento de T. cognata, em plantios experimentais de camu-camu. Foram selecionados, de forma aleatória, 17 e 14 exemplares nos plantios I e II, respectivamente. Para cada uma das variáveis estudadas, foram calculados a média aritmética, o desviopadrão, a variância e a amplitude de variação. Foi verificado um nível de infestação de 82% (plantio I e 57% (plantio II, uma intensidade de infestação de 94% (plantio I e 75% (plantio II e uma média de seis ninfas/folha em cada plantio, o que indica que T. cognata representa uma das pragas-chave dessa cultura. Foram observados adultos de Chrysoperla sp. (Neuroptera: Chrysopidae e ninfas de Reduviidae (Hemiptera: Heteroptera, que podem atuar como prováveis agentes de controle biológico de T. cognata.The cultivation of camu-camu Myrciaria dubia (H.B.K. Mc Vaugh has presented countless phytosanitary problems, among them, Tuthillia cognata Hodkinson et al. (Hemiptera: Homoptera, Psyllidae, which often is noticed as a secondary pest. This study aimed to determine the level and the intensity of infestation (% for T. cognata, as well as to study the biological cycle and behavioural aspects of T. cognata, in experimental plantation of camu-camu. 17 and 14 specimens from plantation I and II, respectively, were randomly selected. The arithmetic average, the standard deviation, the variance and the total amplitude were calculated for each studied variable. A level of infestation of 82% (plantation I and 57% (plantation II, an intensity of infestation of 94% (plantation I and 75% (plantation II and an

  9. Atração e desenvolvimento de Leptoglossus gonagra (Fabr. (Hemiptera: Coreidae em cultivares de abóbora e moranga

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    Baldin Edson Luiz Lopes

    2002-01-01

    Full Text Available As plantas da família Cucurbitaceae são seriamente prejudicadas pelo ataque de insetos, sendo que os danos ocorrem desde a germinação até a colheita e podem ser observados em todas as partes da planta. Dentre os insetos sugadores que atacam a abóbora (Cucurbita moschata e a moranga (C. maxima, as formas jovens e adultas do percevejo Leptoglossus gonagra (Fabr. (Hemiptera: Coreidae merecem destaque por sugarem a seiva das folhas, ramos e frutos novos, nos quais causam necroses, reduzindo a produção. Visando comparar a atratividade de plântulas de cultivares de abóbora e moranga a adultos deste percevejo e os efeitos desses materiais sobre a biologia de ninfas dessa espécie, realizaram-se ensaios sob condições de laboratório. Em teste de atratividade, o cultivar de abóbora BRA015113 destacou-se como o menos atrativo em relação ao cultivar de moranga Exposição, enquanto que o cultivar de abóbora BRA003531 foi o mais atrativo. Todos os cultivares provocaram 100% de mortalidade das ninfas, indicando a presença de componentes antibióticos, adversos ao desenvolvimento de L. gonagra.

  10. Pre shipping dip treatments using soap, natural oils, and Isaria fumosorosea: potential biopesticides for mitigating the spread of whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) invasive insects on ornamental plants

    Science.gov (United States)

    The whitefly Bemisia tabaci (Hemiptera: Aleyodidae) is an invasive insect pest affecting different crops including vegetables, fruits, cereals, and ornamentals. The efficacy of some products such as commercial soap, natural oils and Preferal® (based on the entomopathogenic fungus Isaria fumosorosea ...

  11. Occurrence of the parasitoid Anastatus sp. in eggs of Leptoglossus zonatus under the maize in Brazil Ocorrência do parasitóide Anastatus sp. em ovos de Leptoglossus zonatus em milho no Brasil

    Directory of Open Access Journals (Sweden)

    Carlos Henrique Marchiori

    2003-08-01

    Full Text Available The objective of this study was to report, for the first time in Brazil, the occurrence of the parasitoid Anastatus sp. (Hymenoptera: Eupelmidae in eggs of Leptoglossus zonatus (Hemiptera: Coreidae on maize (Zea mays L. in Itumbiara County, State of Goias, Brazil (18°25'S; 49°13'W. Percent parasitism was 6.9%.O objetivo dessa nota é registrar a primeira ocorrência, no Brasil, do parasitóide Anastatus sp. (Hymenoptera: Eupelmidae em ovos de Leptoglossus zonatus (Hemiptera: Coreidae em cultivar de milho em Itumbiara, Estado de Goiás (18°25'S; 49°13'W. A porcentagem de parasitismo foi de 6,9%.

  12. Intraguild predation by the generalist predator <em>Orius majusculus em>on the parasitoid <em>Encarsia formosaem>

    DEFF Research Database (Denmark)

    Sohrabi, Fariba; Enkegaard, Annie; Shishehbor, Parviz

    2013-01-01

    Intraguild predation of Orius majusculus (Reuter) (Heteroptera: Anthocoridae) on Encarsia formosa (Gahan) (Hymenoptera: Aphelinidae), both natural enemies of Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae), was studied under laboratory conditions. The experiments quantified prey consumption b...

  13. Dinâmica populacional de Bemisia tabaci biótipo B em tomate monocultivo e consorciado com coentro sob cultivo orgânico e convencional Population dynamic of Benisua tabaci B biotype in monoculture tomato crop and consortium with coriander in organic and conventional crop system

    Directory of Open Access Journals (Sweden)

    Pedro HB Togni

    2009-06-01

    Full Text Available A mosca-branca Bemisia tabaci Biótipo B (Hemiptera: Aleyrodidae, é um herbívoro de difícil controle devido à alta plasticidade genotípica da espécie. No tomateiro pode causar danos severos principalmente pela transmissão de diversas viroses. O manejo do sistema de produção e o consórcio de culturas podem ter um efeito direto nas populações desse herbívoro, sem que seja necessária a aplicação de inseticidas. Avaliou-se a influência dos sistemas de produção orgânico e convencional e o consórcio tomate-coentro na dinâmica populacional da mosca-branca no campo experimental da Embrapa Hortaliças, de maio a setembro/06. O monitoramento dos adultos da mosca-branca e de seus inimigos naturais foi realizado utilizando-se armadilhas adesivas amarelas fixadas nas bordas e no interior das parcelas experimentais e a amostragem de ninfas foi realizada por observação direta das folhas de tomate no campo. Embora as populações ao redor dos diferentes tratamentos fossem equivalentes, a abundância de adultos de mosca-branca foi significativamente menor nas parcelas de tomate consorciado com coentro, tanto no sistema convencional como orgânico. Apenas o consórcio tomatecoentro em sistema orgânico apresentou redução significativa na quantidade de ninfas por planta em relação aos demais tratamentos. Os inimigos naturais foram significativamente mais abundantes em sistema orgânico e foi verificada uma correlação negativa da abundância dos inimigos naturais e a quantidade de ninfas por planta. A associação tomate-coentro e o manejo orgânico do agroecossistema favoreceram ao controle biológico natural da mosca-branca.Due to its high genotypic plasticity, the control of the silverleaf whitefly Bemisia tabaci B biotype (Hemiptera: Aleyrodidae, is difficult. This insect may cause severe damage to the tomato crop as a vector of several viruses. The management of the production system and the consortium with other crops may have a

  14. Aspectos biológicos de Chrysoperla externa (Hagen, 1861 (Neuroptera: Chrysopidae alimentada com Bemisia tabaci (Gennadius, 1889 biótipo B (Hemiptera: Aleyrodidae criada em três hospedeiros Biological aspects of Chrysoperla externa (Hagen, 1861 (Neuroptera: Chrysopidae fed on Bemisia tabaci (Gennadius, 1889 biotype B (Hemiptera: Aleyrodidae reared in three hosts

    Directory of Open Access Journals (Sweden)

    Cláudio Gonçalves Silva

    2004-04-01

    Full Text Available Estudaram-se alguns aspectos biológicos de adultos de Chrysoperla externa (Hagen oriundos de larvas alimentadas com ninfas da mosca-branca Bemisia tabaci (Gennadius biótipo B, criadas em folhas de pepino (Cucumis sativus var. caipira, couve (Brassica oleraceae var. acephala e na planta invasora leiteiro (Euphorbia heterophylla. Discos foliares dos hospedeiros contendo ninfas de terceiro e quarto estádios da mosca-branca foram acondicionados em placas de Petri contendo ágar-água a 1%. Em cada placa foi colocada uma larva de C. externa recém-eclodida, onde permaneceram até a emergência dos adultos. Foram avaliados o peso logo após a emergência, os períodos de pré-oviposição, oviposição, efetivo de oviposição, pós-oviposição, número total de ovos produzidos por fêmea e longevidade. Avaliaram-se, também, o período embrionário e a viabilidade dos ovos, coletando-se, uma vez por semana, uma amostra de 10% do total de ovos produzidos no dia, ao longo de todo o período reprodutivo. O peso de machos e fêmeas não diferiu significativamente em função do hospedeiro da presa, sendo em média de 4,7 mg. De maneira geral, adultos de C. externa oriundos de larvas alimentadas com ninfas de B. tabaci biótipo B criadas em leiteiro apresentaram performance inferior em relação àqueles oriundos do pepino e da couve. O período de oviposição foi, em média, de 49,5 dias para o pepino e a couve, e de 31,6 dias para o leiteiro. A produção de ovos foi reduzida em aproximadamente 50% em leiteiro, sendo de 293,8 ovos; no pepino e couve, a média foi de 591,3 ovos. Ninfas de B. tabaci biótipo B criadas em leiteiro causaram uma redução da viabilidade dos ovos de C. externa, que foi 62,8% e 57,7% inferior ao verificado para o pepino e a couve, respectivamente.The aim was to study some biological aspects of adults of Chrysoperla externa (Hagen, from larvae fed on nymphs of third and fourth stages of Bemisia tabaci (Gennadius biotype B

  15. Responses of Neomegalotomus parvus (Hemiptera: Alydidae to color and male-lured traps

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    Maurício Ursi Ventura

    2004-08-01

    Full Text Available In 1998, the relative responses of Neomegalotomus parvus (Westwood (Hemiptera: Alydidae to colors in traps were assessed in the field. Colors were printed in white alkaline paper and covered with transparent plastic. Printed colors were black, green, yellow, red and blue. Treatments also included white paper and aluminum foil. Green traps captured more bugs than the other traps (except yellow. Yellow traps caught significantly more N. parvus males than blue, black and aluminum traps. White, red and blue traps captured significantly more N. parvus males than black traps. In 1999, the same treatments were used and additionally included the factor presence (X absence of males in cages in the traps. Four males were confined in the cages together with pigeon pea seeds and water. Control traps received only pigeon pea seeds and water. No responses were found for color comparisons. Male-lured traps captured significantly more males than control traps.Em 1998, as respostas relativas de Neomegalotomus parvus (Westwood (Hemiptera: Alydidae a cores em armadilhas foram avaliadas no campo. As cores foram impressas (preto, verde, amarelo, vermelho e azul, em papel alcalino branco e coberto com plástico transparente. Também se incluiu papel branco e folha de alumínio, como tratamentos. Armadilhas verdes capturaram mais insetos que outras armadilhas (exceto amarelo. Armadilhas amarelas capturaram significativamente mais machos de N. parvus do que armadilhas azuis, pretas e alumínio. Armadilhas brancas, vermelhas e azuis capturaram significativamente mais machos de N. parvus do que armadilhas pretas. Em 1999, os mesmos tratamentos foram usados e também se incluiu fator presença (X ausência de machos em gaiolas nas armadilhas. Quatro machos foram confinados nas gaiolas junto com sementes de guandu e água. Testemunhas receberam somente sementes de guandu e água. Não foram obtidas respostas significativas à cores. Armadilhas com machos capturaram

  16. Plano de amostragem do biótipo B de Bemisia tabaci na cultura do pepino

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    Moura Marcelo Fialho de

    2003-01-01

    Full Text Available A adoção de programas de manejo integrado de pragas permite reduzir a aplicação de inseticidas e os planos convencionais de amostragem representam o ponto inicial na geração desses programas. Assim, o objetivo deste trabalho foi determinar plano de amostragem convencional para o biótipo B da mosca-branca Bemisia tabaci Genn. (Hemiptera: Aleyrodidae na cultura do pepino. Foram avaliadas as densidades de ninfas e de adultos em dez lavouras comerciais de pepino, Tocantins, MG, estudando nove sistemas amostrais formados pela combinação de três técnicas (batida de folha em bandeja de plástico branco, contagem direta dos insetos na face inferior da folha e coleta de folha em sacola de plástico e três unidades amostrais (folha do terço apical, mediano ou basal do espaldeiramento. A contagem direta em folha do terço basal foi o sistema com maior precisão econômica na amostragem de ninfas, mas não possibilitou a geração de plano de amostragem praticável. As batidas, em bandeja, de folha dos terços apicais, medianos ou basais do espaldeiramento foram os sistemas economicamente mais precisos na amostragem de adultos. Desses, apenas a batida de folha do terço mediano em bandeja gerou plano de amostragem praticável, sendo que este plano é composto de 196 amostras/lavoura.

  17. Efficiency of neem oil nanoformulations to Bemisia tabaci (GENN. Biotype B (Hemiptera: AleyrodidaeEficiência de nanoformulações a base de óleo de nim sobre Bemisia tabaci (GENN. Biótipo B (Hemiptera: Aleyrodidae

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    Sheila Salles Carvalho

    2012-04-01

    Full Text Available The nanotechnology, through encapsulation of active ingredients, has showed an important way to avoid problems with quickly degradation of the pesticide molecules. Thus, neem (Azadirachta indica oil nanoformulations containing ?-ciclodextrin and poli-?-caprolactone (PCL were tested as to their control efficiency against eggs and nymphs of Bemisia tabaci (Genn. biotype B reared in soybean. The Lethal Concentration (LC50 was estimated using a commercial neem oil (Organic Neem® on first-instar nymphs to establish the adequate volume of the nanoformulations per treatment. After that, they were sprayed on eggs and first-instar nymphs in laboratory and greenhouse and on third-instar nymphs in greenhouse. The commercial neem oil and distilled water were used as controls. Egg viability was not affected by any treatment. Among six nanoformulations, only one was efficient against the first-instar nymphs in laboratory conditions. However, its effective period was not increased as expected. In greenhouse, first-instar nymphs were more affected by two nanoformulations which were significantly different of the commercial neem oil - the most effective one. No mortality differences among the formulations in the third-instar test were observed. The nanoformulations were less efficient to control the B. tabaci biotype B nymphs than the commercial neem oil. A nanotecnologia, através do encapsulamento de ingredientes ativos, tem-se revelado uma importante estratégia para evitar problemas com a rápida degradação de moléculas inseticidas. Assim, nanoformulações à base de óleo de nim (Azadirachta indica utilizando os polímeros ?-ciclodextrina e poli-?-caprolactona (PCL foram testadas quanto a sua eficiência de controle de ovos e ninfas de Bemisia tabaci (Genn. biótipo B mantidas em soja. Foi estimada a CL50 utilizando uma formulação comecial de óleo de nim (Organic Neem® sobre ninfas em 1º ínstar da qual se estipulou o volume das nanoformula

  18. Insecticide effects of Ruta graveolens, Copaifera langsdorffii and Chenopodium ambrosioides against pests and natural enemies in commercial tomato plantation = Ação inseticida de Ruta graveolens, Copaifera langsdorffii e Chenopodium ambrosioides sobre pragas de tomate

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    Flávia Silva Barbosa

    2011-01-01

    Full Text Available The aim of this study was to evaluate the insecticide effect of watery leaf extracts of Ruta graveolens (Rutaceae, alcoholic leaf extracts of Copaifera langsdorffii (Caesalpinaceae and Chenopodium ambrosioides (Chenopodiaceae in the concentration of 5% under field conditions. The experiment design was randomized blocks with six replications. The parcels treated with plant extracts showed reduction in the population of pests when compared with the controlparcels. The extract elaborated with C. langsdorffii presented greater insecticidal effect under Bemisia tabaci (Hemiptera: Aleyrodidae and sum of pests. It was verified that after 24 hours ofspraying, the parcels treated with the extract of C. ambrosioides presented minor numbers of adults of Tuta absoluta (Lepidoptera: Gelechiidae, followed by the parcels treated with extract of R.graveolens. There were smaller numbers of parasitoid eggs of epidopterans Trichogramma sp. (Hymenoptera: Trichogrammatidae and sum of natural enemies (predators + parasitoids in the parcels that had received spraying with extracts from the plants of C. langsdorffii and C. ambrosioides, followed by R. graveolens, compared to the control. There were a smaller number of parasitoids from the family Eulophidae (Hymenoptera attacking caterpillars of T. absoluta in plants treated with R. graveolens, followed by C. langsdorffii and C. ambrosioides than in the control.O objetivo deste trabalho foi avaliar o efeito inseticida dos extratos aquosos de folhas de Ruta graveolens (Rutaceae e alcoólicos de folhas de Copaifera langsdorffii (Caesalpinaceae e de folhas de Chenopodium ambrosioides (Chenopodiaceae a 5%. O experimento foi em blocos casualizados com seis repetições. As parcelas tratadas tiveram redução na população de pragas, quando comparadas às parcelas sem nenhum tratamento. O extrato elaborado com C. langsdorffii apresentou maior efeito inseticida em Bemisia tabaci (Hemiptera: Aleyrodidae e soma das pragas

  19. Presencia de Diaphorina citri (Hemiptera: Psyllidae en el Noroeste Argentino (NOA Record of Diaphorina citri (Hemiptera: Psyllidae in North West Argentina

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    Lucrecia Augier

    2006-12-01

    Full Text Available Se amplía la distribución de Diaphorina citri Kuwayama (Hemiptera: Psyllidae para el Noroeste Argentino.The distribution of Diaphorina citri Kuwayama (Hemiptera: Psyllidae is expanded to North West Argentina.

  20. Phytophagy on eucalyptus plants increases the development and reproduction of the predator Podisus nigrispinus (Hemiptera: Pentatomidae = Fitofagia em plantas de eucaliptos aumenta o desenvolvimento e a reprodução do predador Podisus nigrispinus (Hemiptera: Pentatomidae.

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    Anderson Mathias Holtz

    2011-04-01

    Full Text Available The effect of plant feeding on biological aspects of Podisus nigrispinus (Dallas (Heteroptera: Pentatomidae was evaluated. Nymphs and adults of this predator were fed with Tenebrio molitor L. (Coleoptera: Tenebrionidae pupae on Eucalyptus urophylla plants inthe field or only with pupae of this prey in the laboratory. The development and nymphal survival, as well as the pre-oviposition period, number of egg masses, number, fertility and viability of eggs and the longevity of females of this predator were evaluated. The eucalyptus plants improved the development of P. nigrispinus. This demonstrates that this predator can present higher population growth with eucalyptus plants and T. molitor pupae than in the laboratory (controlled conditions only with this prey. These plants can supply nutrients that can the population growth and efficiency of P. nigrispinus for biological control in eucalyptus plantations.O efeito da alimentação em plantas sobre os aspectos biológicos de Podisus nigrispinus (Hemiptera: Pentatomidae foi avaliado. Ninfas e adultos desse predador foram alimentados com pupas de Tenebrio molitor L. (Coleotpera: Tenebrionidae em plantas de Eucalyptus urophylla no campo ou, apenas, em laboratório. O desenvolvimento e a sobrevivência ninfal desse predador, além do período de pré-oviposição, número de posturas, viabilidade dos ovos e afertilidade e longevidade de fêmeas foram avaliados. A planta de eucalipto proporcionou um incremento no desenvolvimento de P. nigrispinus. Isto demonstra que esse predador pode apresentar maior crescimento populacional com plantas de eucalipto e pupas de T. molitor alimentado com apenas a presa (condições controladas. A planta pode fornecer nutrientes que aumentam o crescimento populacional e a eficiência de P. nigrispinus para o controle biológico em plantios de eucalipto.

  1. Factors affecting colonization and abundance of Aphis gossypii glover (hemiptera: aphididae on okra plantations Fatores que afetam a colonização e abundância de Aphis gossypii glover (Hemiptera: Aphididae em plantações de quiabeiro

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    Germano Leão Demolin Leite

    2007-04-01

    Full Text Available The control of Aphis gossypii Glover (Hemiptera: Aphididae on okra Abelmoschus esculentus (L. (Malvaceae consist primarily in the use of insecticides, due to the lack of information on other mortality factors. The objective of this study was to determine the effects of predators and parasitoids, height of canopy, plant age, leaf areas, organic compounds leaves, levels of leaf nitrogen and potassium, density of leaf trichomes, total rainfall and median temperature on attack intensity of A. gossypii on two successive A. esculentus var. Santa Cruz plantations. Monthly number estimates of A. gossypii and natural enemies (visual inspection occurred on bottom, middle and apical parts of 30 plants/plantation (one leaf/plant. Plants senescence, leaf areas and natural enemies, mainly Adialytus spp., spiders and Coccinellidae, were some of the factors that most contributed to aphid reduction. A higher number of aphids was found on the bottom part than medium and apical parts of okra plants. Total rainfall can reduce the aphid population. Trichomes non-glandular or low density, organic compounds leaves and levels of N and K were not important for reducing aphid population.O controle de Aphis gossypii Glover (Hemiptera: Aphididae em quiabeiro Abelmoschus esculentus (L. (Malvaceae consiste principalmente no uso de inseticidas, em virtude da falta de informação sobre outros fatores de mortalidade. Objetivou-se com este estudo determinar os efeitos de predadores e parasitóides, altura de dossel, idade da planta, área foliar, compostos orgânicos foliares, níveis de nitrogênio e potássio, densidade de tricomas, pluviosidade e temperatura na intensidade de ataque de A. gossypii em dois cultivos sucessivos de Abelmoschus esculentus var. Santa Cruz. Estimou-se, mensalmente, o número de A. gossypii e de inimigos naturais (inspeção visual ocorridos nas folhas (uma folha/planta localizadas nas partes basal, mediana e apical de 30 plantas/plantação. Os

  2. Controle químico de ovos e ninfas de Bemisia tabaci biótipo B (Hemiptera: aleyrodidae Chemical control of Bemisia tabaci B biotype (Hemiptera: aleyrodidae eggs and nymphs

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    Giuliana Etore do Valle

    2002-06-01

    Full Text Available Moscas brancas são pragas de diversas culturas, causam redução na produção e na qualidade do produto. Inseticidas, com diferentes modos de ação vêm sendo utilizados, sem muito sucesso. Neste trabalho avaliou-se a eficiência de inseticidas sobre ovos de diferentes idades (1, 3 e 5 dias e de ninfas de 1° ínstar da mosca branca Bemisia tabaci biótipo B. Os experimentos foram realizados em condições de laboratório, sob temperatura de 25 ± 2°C e fotofase de 12 horas. Piriproxifem (75 mg L-1 atuou como excelente ovicida, sendo altamente eficiente, 3 dias de idade. Cartape (500 mg L-1 não teve efeito satisfatório, mas a 1000 mg L-1 foi eficiente em ovos com até 5 dias de idade. Quanto à ação sobre ninfas de 1° ínstar, para pulverizações na face superior das folhas, piriproxifem, a 75 mg L-1, apresentou eficiência na supressão da emergência de adultos, caracterizando presença de ação translaminar; buprofezim, cartape, acefato e fempiroximate não apresentaram eficiência no controle de ninfas. Quando aplicado na face inferior das folhas, buprofezim (375 mg L-1 apresentou eficiência comparável à de piriproxifem, impedindo a emergência de adultos.Whiteflies are pests of many crops, cause severe yield losses and reduce crop quality. Insecticides of different modes of action have been used without success. In this work, the efficiency of five insecticides on Bemisia tabaci B biotype (= B. argentifolii eggs and nymphs was evaluated under laboratory conditions (T = 25 ± 2°C; 12 h photophase. In relation to the ovicidal effect, pyriproxyfen (75 mg L-1 caused egg hatching inhibition up to three day old eggs. Cartap showed efficiency only at the concentration of 1000 mg L-1, supressing the viability of eggs up to five days. Soybean leaves with first instar nymphs were treated in the upper surface with pyriproxyfen (75 mg L-1, buprofezin (375 mg L-1, cartap (1000 mg L-1, acephate (750 mg L-1 and fenpyroximate (100 mg L-1; in

  3. Gender- and species-specific characteristics of bacteriomes from three psyllid species (Hemiptera: Psylloidae)

    Science.gov (United States)

    Psyllids (Hemiptera: Pyslloidea) harbor bacterial symbionts in specialized organs called bacteriomes. Bacteriomes may be subject to manipulation to control psyllid pests including Bactericera cockerelli (Sulc) (Hemiptera: Triozidae) and Cacopsylla pyricola (Forster) (Hemiptera: Psyllidae) if the bi...

  4. Record of Diaphorina citri (Hemiptera: Psyllidae) in North West Argentina

    OpenAIRE

    Augier, Lucrecia; Gastaminza, Gerardo; Lizondo, Marcelo; Argañaraz, Manuel; Willink, Eduardo

    2006-01-01

    Se amplía la distribución de Diaphorina citri Kuwayama (Hemiptera: Psyllidae) para el Noroeste Argentino.The distribution of Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is expanded to North West Argentina.

  5. Perdas causadas por Coccus viridis (Hemiptera: Coccidae em mudas de Coffea arabica L.

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    Flávio Lemes Fernandes

    2009-08-01

    Full Text Available Coccus viridis (Green danifica plantas jovens e adultas de Coffea arabica Linnaeu. No entanto, nada se sabe sobre a magnitude dos danos causados por esta praga. Assim, este trabalho teve por objetivo estudar as relações entre o ataque de C. viridis e as perdas causadas por este inseto a C. arabica. Este trabalho foi conduzido em casa de vegetação na Universidade Federal de Viçosa. Foram utilizadas sementes da linhagem IAC 15 da variedade “Catuaí vermelho” de café (C. arabica. Para a confecção dos tratamentos esta praga foi criada em casa de vegetação separada do experimento. Os tratamentos foram: plantas infestadas e não infestadas por adultos e ninfas da cochonilha verde. As plantas foram nutridas com solução nutritiva. Durante 110 dias foram avaliados: números de adultos e de ninfas de primeiro, segundo e terceiro ínstares, área foliar, diâmetro do caule, altura das plantas em todas repetições. No final do experimento avaliou-se o peso das raízes, caule, folhas e total. Os pesos das raízes, matéria seca total, área foliar e diâmetro do caule de plantas não atacadas por C. viridis superaram em 1,31; 1,41; 1,50 e 8,93 vezes, respectivamente o peso de plantas atacadas. As variáveis selecionadas foram: diâmetro do caule (cm, área foliar (cm², peso de raízes (g, ninfas, adultos e total das cochonilhas. Concluindo que a planta de C. arabica é afetada de forma diferente entre seus órgãos e que a ninfa de terceiro ínstar e adultos são as fases que mais causam danos a C. arabica.Losses Caused by Coccus viridis (Green (Hemiptera: Coccidae on Seedlings of Coffea arabica L.Abstract. Coccus viridis (Green cause losses on seedling and old plants of Coffea arabica (Green. However, nothing is known about of the damages caused by this pest. Thus, the objective of this work was to evaluate the relations between atack of C. viridis and the losses caused by this insect. on C. arabica. This work was conduced in greenhouse at

  6. A Molecular Phylogeny of Hemiptera Inferred from Mitochondrial Genome Sequences

    Science.gov (United States)

    Song, Nan; Liang, Ai-Ping; Bu, Cui-Ping

    2012-01-01

    Classically, Hemiptera is comprised of two suborders: Homoptera and Heteroptera. Homoptera includes Cicadomorpha, Fulgoromorpha and Sternorrhyncha. However, according to previous molecular phylogenetic studies based on 18S rDNA, Fulgoromorpha has a closer relationship to Heteroptera than to other hemipterans, leaving Homoptera as paraphyletic. Therefore, the position of Fulgoromorpha is important for studying phylogenetic structure of Hemiptera. We inferred the evolutionary affiliations of twenty-five superfamilies of Hemiptera using mitochondrial protein-coding genes and rRNAs. We sequenced three mitogenomes, from Pyrops candelaria, Lycorma delicatula and Ricania marginalis, representing two additional families in Fulgoromorpha. Pyrops and Lycorma are representatives of an additional major family Fulgoridae in Fulgoromorpha, whereas Ricania is a second representative of the highly derived clade Ricaniidae. The organization and size of these mitogenomes are similar to those of the sequenced fulgoroid species. Our consensus phylogeny of Hemiptera largely supported the relationships (((Fulgoromorpha,Sternorrhyncha),Cicadomorpha),Heteroptera), and thus supported the classic phylogeny of Hemiptera. Selection of optimal evolutionary models (exclusion and inclusion of two rRNA genes or of third codon positions of protein-coding genes) demonstrated that rapidly evolving and saturated sites should be removed from the analyses. PMID:23144967

  7. A molecular phylogeny of Hemiptera inferred from mitochondrial genome sequences.

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    Nan Song

    Full Text Available Classically, Hemiptera is comprised of two suborders: Homoptera and Heteroptera. Homoptera includes Cicadomorpha, Fulgoromorpha and Sternorrhyncha. However, according to previous molecular phylogenetic studies based on 18S rDNA, Fulgoromorpha has a closer relationship to Heteroptera than to other hemipterans, leaving Homoptera as paraphyletic. Therefore, the position of Fulgoromorpha is important for studying phylogenetic structure of Hemiptera. We inferred the evolutionary affiliations of twenty-five superfamilies of Hemiptera using mitochondrial protein-coding genes and rRNAs. We sequenced three mitogenomes, from Pyrops candelaria, Lycorma delicatula and Ricania marginalis, representing two additional families in Fulgoromorpha. Pyrops and Lycorma are representatives of an additional major family Fulgoridae in Fulgoromorpha, whereas Ricania is a second representative of the highly derived clade Ricaniidae. The organization and size of these mitogenomes are similar to those of the sequenced fulgoroid species. Our consensus phylogeny of Hemiptera largely supported the relationships (((Fulgoromorpha,Sternorrhyncha,Cicadomorpha,Heteroptera, and thus supported the classic phylogeny of Hemiptera. Selection of optimal evolutionary models (exclusion and inclusion of two rRNA genes or of third codon positions of protein-coding genes demonstrated that rapidly evolving and saturated sites should be removed from the analyses.

  8. Keanekaragaman dan Kelimpahan Musuh Alami Bemisia tabaci (Gennadius (Hemiptera: Aleyrodidae pada Pertanaman Cabai Merah di Kecamatan Pakem, Kabupaten Sleman, Daerah Istimewa Yogyakarta

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    Hendrival Hendrival

    2015-09-01

    Full Text Available Research on natural enemies of Bemisia tabaci was conducted in the chili pepper fields in Sub-district of Pakem, District of Sleman, The Special Province of Yogyakarta during the dry season of May-October 2009. The aims of this research were to study the diversity and abundance of parasitoid and predator species associated with B. tabaci. Samplings of insect species were done using yellow pan trap, sweep net, direct observation of insects colonized young leaves, and collection of nymphs for B. tabaci. Measurement of insect diversity was calculated using Shannon’s index diversity and Evenness index. Nine species of insect predator were identified, i.e. Harmonia octomaculata (Fabricius, Menochilus sexmaculata (Fabricius, Scymnus sp., Micraspis inops Mulsant, Coccinella sp. (Coleoptera: Coccinellidae, Paederus fuscipes Curtis (Coleoptera: Staphylinidae, Orius sp. (Hemiptera: Anthocoridae, Linyphiidae sp.1 (Araneae, and Syrphidae sp.1 (Diptera. Eretmocerus sp. (Hymenoptera: Aphelinidae was the only parasitoid found in the nymphs B. tabaci collected and has the potential to control B. tabaci in the red pepper fields.

  9. Biologia do psilídeo-de-concha Glycaspis brimblecombei Moore (Hemiptera, Psyllidae em Eucalyptus spp. Red gum lerp psyllid Glycaspis brimblecombei Moore (Hemiptera, Psylidae biology in Eucalyptus spp.

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    Daniela Cristina Firmino-Winckler

    2009-01-01

    Full Text Available Biologia do psilídeo-de-concha Glycaspis brimblecombei Moore (Hemiptera, Psyllidae em Eucalyptus spp.. Glycaspis brimblecombei Moore também conhecido por psilídeo-de-concha, se caracteriza por ser uma espécie específica ao gênero Eucalyptus L'Her. Este trabalho teve por objetivo determinar o ciclo biológico de G. brimblecombei em Eucalyptus spp. O trabalho foi conduzido em câmara climatizada (BOD, sob a temperatura de 26 °C e fotofase de 12 horas. As espécies de Eucalyptus utilizadas para o experimento foram: Eucalyptus camaldulensis, E. tereticornis, E. urophylla, E. grandis, Corymbia citriodora e um híbrido de E. grandis x E. urophylla ('urograndis'. Inicialmente foram utilizados 100 repetições (ninfas tratamento (espécies de Eucalyptus. As avaliações foram diárias. Os parâmetros biológicos avaliados foram a duração e viabilidade do estágio ninfal, longevidade dos adultos, número de posturas/fêmea, duração do período embrionário, número e viabilidade ovos, longevidade dos adultos e duração do ciclo total. Os resultados obtidos demonstraram que salvo C. citriodora que apresentou ser letal ao desenvolvimento ninfal de G. brimblecombei as demais espécies de Eucalyptus testadas oferecem condições ao desenvolvimento biológico deste psilídeo, sendo que neste trabalho E. camaldulensis mostrou-se a mais adequada.Glycaspis brimblecombei Moore, also known as red gum lerp psyllid, is characterized to be specific to the genus Eucalyptus. This work aimed to evaluate G. brimblecombei biological cycle in Eucalyptus spp. The work was accomplished in acclimatized chamber (BOD, with temperature of 26 °C and photophase of 12 hours. Eucalyptus species used in this study were: Eucalyptus camaldulensis, E. tereticornis, E. urophylla, E. grandis, Corymbia citriodora and E grandis x E. urophylla hybrid ('urograndis'. Initially 100 replications (nymphs per treatment (Eucalyptus species were prepared. The evaluations were daily and

  10. Factors affecting oviposition of Bemisia tabaci (Genn.) biotype B (Hemiptera: Aleyrodidae) in sweet pepper; Fatores que afetam a oviposicao de Bemisia tabaci (Genn.) biotipo B (Hemiptera: Aleyrodidae) em pimentao

    Energy Technology Data Exchange (ETDEWEB)

    Lima, Larissa C. de; Campos, Alcebiades R. [UNESP, Ilha Solteira, SP (Brazil). Dept. de Fitossanidade, Engenharia Rural e Solos]. E-mail: lclima@aluno.feis.unesp.br; campos@bio.feis.unesp.br

    2008-03-15

    Bemisia tabaci (Gen.) biotype B is considered a pest of economical importance for several vegetables. The oviposition behaviour of the while fly was evaluated in sweet pepper plants. The trials were carried out under greenhouse condition and in the Laboratory of Entomology of DEFERS/ UNESP, Campus of Ilha Solteira-SP, with the sweet pepper Magali-R genotype. The effect of plant age on the whitefly oviposition was evaluated in free-choice tests, in plants, 25, 30, 35, 40 and 45- day-old, as egg distribution in the plant and on the leaf blade was evaluated in 35-days-old plants. In a no-choice tests, 35-day-old plants were used to evaluate the effect of the densities of 50, 100, 150, 200 and 250 adults per plant on the number of eggs laid by insects. The silver leaf whitefly preferred to oviposition the third to sixth leaflets, of the medium and superior part of plants of sweet pepper; the leaf blade areas, located in the lobes right and left close the base of the leaf were the preferential site for whitefly oviposition. Older plants, 40- and 45-day-old, were preferentially used for oviposition, and 200 and 250 adults per plant were both enough to lay a number of eggs that allowed to differentiate among sweet pepper genotypes with different whitefly resistance levels. (author)

  11. Biological aspects of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on six bean genotypes; Aspectos biologicos de Bemisia tabaci (Genn.) biotipo B (Hemiptera: Aleyrodidae) em seis genotipos de feijoeiro

    Energy Technology Data Exchange (ETDEWEB)

    Oriani, Maria A. de G. [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Ecologia e Biologia Evolutiva; Vendramin, Jose D. [Universidade de Sao Paulo (USP), Piracicaba, SP (Brazil). Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Dept. de Entomologia, Fitopatologia e Zoologia Agricola; Brunherotto, Rogerio [Fundacao Municipal de Ensino Superior de Braganca Paulista, SP (Brazil)

    2008-03-15

    The silverleaf whitefly is one of the most harmful pests that attack bean crops, mainly for extracting large quantities of phloem sap and transmitting the bean golden mosaic virus. Resistant germplasm plants can be an important method for controlling this pest. The biological aspects of Bemisia tabaci B biotype on bean (Phaseolus vulgaris) genotypes were evaluated. The tests were conducted under laboratory conditions, with the following genotypes: Arc 1, Arc 3s, Arc 5s, G13028, G11056 and Porrillo 70. The bean plants in a stage IV-1 were infested during one day with silverleaf white flies. Afterwards the eggs and nymphs were observed until adult emergence. Longevity and fecundity of emerged insects were also evaluated. The longest development time occurred for nymphs fed on Arc 3s genotype (26.5 days), following by G11056 (25.9 days) and G13028 (25.3 days). The development period was 5.5 days longer in Arc 3s when compared with Porrillo 70. Also, the wild genotypes Arc 3s and G11056 showed higher mortality rates (94.7% and 83.1%, respectively), which may suggest antibiosis and/or feeding non preference resistance type. For this reason, although longevity and fecundity were not influenced when the whitefly fed on resistant genotypes (Arc 3s, G11056, G13028 and Arc 5s), those genotypes can be used for bean breeding program towards B. tabaci B biotype. (author)

  12. Biology of the citrus blackfly, Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae), in three host plants; Biologia da mosca-negra-dos-citros, Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae), em tres plantas hospedeiras

    Energy Technology Data Exchange (ETDEWEB)

    Pena, Marcia R.; Silva, Neliton M. da [Universidade Federal do Amazonas (UFAM), Manaus, AM (Brazil).Lab. de Entomologia Agricola], e-mail: marciarpena@yahoo.com.br, e-mail: nmarques@ufam.edu.br; Venframim, Jose D.; Haddad, Marineia de L. [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil). Lab. de Resistencia de Plantas e Plantas Inseticidas], e-mail: jdvendra@esalq.usp.br, e-mail: mlhaddad@esalq.usp.br; Lourencao, Andre L. [Instituto Agronomico de Campinas (IAC), SP (Brazil)], e-mail: andre@iac.sp.gov.br

    2009-03-15

    The citrus blackfly, Aleurocanthus woglumi Ashby, was detected in Brazil in 2001. The aim of this research was to evaluate the biology, biometry and host preference of A. woglumi in sweet orange, acid lime Tahiti and mango. Experiments were set in laboratory conditions with insects collected in rangpur lime plants in Manaus, State of Amazonas, from January to June of 2006. The following parameters were evaluated: number of spirals (ovo positions) and eggs per plant, number of eggs by spiral per plant, survival of the immature (eggs, 1st, 2nd, 3rd and 4th instars), and length and survival of the immature stage. The mean embrionary period was 15 days for the three hosts. The 4th nymph (puparium) was the longest during nymph development. Second and third instars had the highest survival. The mean length of the egg-adult cycle was 70 days for the three hosts evaluated. The eggs were laid in a spiral shape on the adaxial leaf surface. The 1st instars moved to short distances from the spiral, while the 2nd, 3rd and 4th are sessile and have bristles on the whole body. Based on the highest oviposition and the highest survival of the immature stage of the citrus blackfly in acid lime Tahiti, this plant can be considered the most suitable host to A. woglumi. (author)

  13. Phylogenetic relationships of Hemiptera inferred from mitochondrial and nuclear genes.

    Science.gov (United States)

    Song, Nan; Li, Hu; Cai, Wanzhi; Yan, Fengming; Wang, Jianyun; Song, Fan

    2016-11-01

    Here, we reconstructed the Hemiptera phylogeny based on the expanded mitochondrial protein-coding genes and the nuclear 18S rRNA gene, separately. The differential rates of change across lineages may associate with long-branch attraction (LBA) effect and result in conflicting estimates of phylogeny from different types of data. To reduce the potential effects of systematic biases on inferences of topology, various data coding schemes, site removal method, and different algorithms were utilized in phylogenetic reconstruction. We show that the outgroups Phthiraptera, Thysanoptera, and the ingroup Sternorrhyncha share similar base composition, and exhibit "long branches" relative to other hemipterans. Thus, the long-branch attraction between these groups is suspected to cause the failure of recovering Hemiptera under the homogeneous model. In contrast, a monophyletic Hemiptera is supported when heterogeneous model is utilized in the analysis. Although higher level phylogenetic relationships within Hemiptera remain to be answered, consensus between analyses is beginning to converge on a stable phylogeny.

  14. Does the aggressiveness of the prey modify the attack behavior of the predator Supputius cincticeps (Stål (Hemiptera, Pentatomidae? A agressividade da presa altera o comportamento de ataque do predador Supputius cincticeps (Stål (Hemiptera, Pentatomidae?

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    Rafael Braga da Silva

    2012-06-01

    Full Text Available Does the aggressiveness of the prey modify the attack behavior of the predator Supputius cincticeps (Stål (Hemiptera, Pentatomidae? The stink bug Supputius cincticeps (Stål (Hemiptera, Pentatomidae is a predator found in several Brazilian regions, which possesses desirable attributes as a natural control agent and in biological control programs. The aim of this study was to test if the attack behavior and predation success of S. cincticeps were affected by prey species. Larvae of Tenebrio molitor (L. (Coleoptera, Tenebrionidae, Spodoptera frugiperda (J. E. Smith (Lepidoptera, Noctuidae, and Thyrinteina arnobia (Stoll (Lepidoptera, Geometridae were offered to S. cincticeps in laboratory bioassays where predatory attack and prey defensive behaviors were observed for 2-hour periods. The attack behavior of S. cincticeps changed with the prey species offered. More than 25% of T. molitor and S. frugiperda larvae were immediately attacked, but T. arnobia was not immediately attacked by S. cincticeps. Successful attack (i.e., successful insertion of the predator stylets into the prey depends on the region of the body attacked, with a greater proportion of successful attacks in the anterior than in the median or posterior regions. Larvae of T. arnobia and S. frugiperda displayed a sequence of abrupt head and body movements in response to S. cincticeps attack. Attempts of predation were more successful on T. molitor and S. frugiperda than on T. arnobia. Information about the differential attack behavior of S. cincticeps on different prey species is important for designing successful biological control programs using this hemipteran predator.A agressividade da presa altera o comportamento de ataque do predador Supputius cincticeps (Stål (Hemiptera, Pentatomidae? O percevejo Supputius cincticeps (Stål (Hemiptera, Pentatomidae é um predador encontrado em várias regiões brasileiras, que possui atributos desejáveis como agente de controle natural ou em

  15. Locais e período de alimentação da cigarrinha vetora de Xylella fastidiosa, Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae, em mudas cítricas Feeding sites and food intake of Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae, a sharpshooter vector of Xylella fastidiosa, on citrus plants

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    Marcelo Pedreira de Miranda

    2008-12-01

    Full Text Available A cigarrinha Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae é um importante vetor da bactéria Xylella fastidiosa, agente causal da clorose variegada dos citros. Este trabalho teve como objetivo identificar o local preferido de alimentação e o período de maior atividade alimentar desta cigarrinha em citros, no sentido de elucidar o comportamento alimentar relacionado à transmissão da bactéria. O local de alimentação foi estudado em ensaio de escolha, no qual 30 insetos adultos foram liberados em gaiolas de observação (n = 10 contendo uma muda de laranja-doce [Citrus sinensis (L. Osbeck]. Após 1; 15; 21; 25; 39; 45 e 49 h da liberação, contaram-se os insetos na parte superior (ramos com brotações e inferior (haste principal, até H"40 cm de altura da muda. Nos ramos da parte superior, avaliou-se a preferência entre a haste, o pecíolo e o limbo foliar. Em um segundo ensaio, 20 machos e 20 fêmeas de B. xanthophis foram confinados individualmente sobre a haste de 'seedlings' de laranja-doce para determinar os períodos de alimentação, quantificando-se a excreção de 'honeydew' (medida indireta da ingestão em períodos sucessivos de dia e noite, durante 48 h. A maioria dos indivíduos de B. xanthophis preferiu a haste dos ramos novos (62%, na parte superior da muda cítrica (91%. Nos 'seedlings', observou-se maior volume de excreção e proporção de indivíduos excretando durante a fotofase, independentemente do sexo. Portanto, em estudos de transmissão de X. fastidiosa, deve-se considerar a preferência de B. xanthophis pela haste de brotações cítricas e sua maior atividade alimentar durante a fotofase.The sharpshooter Bucephalogonia xanthophis (Berg (Hemiptera: Cicadellidae is an important vector of Xylella fastidiosa, a bacterium that causes citrus variegated chlorosis. This study aimed to identify the preferred feeding sites and periods of this vector on citrus, in order to improve our understanding of the

  16. Levantamento entomofaunístico de artrópodes em algodoeiro de fibra naturalmente colorida em Ipanguaçu-RN

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    Bárbara Karine de Albuquerque Silva

    2016-08-01

    Full Text Available Objetivou-se com esta pesquisa identificar a diversidade de artrópodes associados à cultura do algodão Gossypium hirsutum L. com pluma colorida, sendo realizados levantamentos entomofaunístico em Ipanguaçu-RN em áreas de produção. A área experimental foi composta por 15 variedades de algodão com pluma colorida: CNPA 2009-6; CNPA 2009-11; CNPA 2009-13; CNPA 2009-16; CNPA 2009-27; CNPA 2009-42; CNPA 2009-47; CNPA 2009-48; CNPA 2009-50; CNPA 2009-59; CNPA 2009-60; CNPA 2009-62; BRS RUBI; BRS AROEIRA; BRS TOPÁZIO. O levantamento foi realizado tendo como base três métodos de coleta ativa em pontos aleatórios da área experimental. As coletas consistiram da retirada manual de folhas e maçãs diretamente da planta, além da captura de insetos em pleno voo, com auxílio de rede entomológica. Foram encontrados um total 1884 insetos adultos e 66 larvas, dispostos em 8 ordens e 22 famílias. A família Aphididae: Hemiptera foi a mais numerosa entre o material coletado, com 1720 adultos dispersos nos três métodos de coleta aplicados. Além desta, outras famílias de pragas da cultura foram encontradas, como Curculionidae: Coleoptera. Também foram coletados organismos benéficos como os polinizadores Aphidae e Megachilidae, pertencentes a ordem Hymenoptera, predadores (Coccinellidae: Coleoptera; Vespidae: Hymenoptera; Reduviidae: Hemiptera; Chrysopidae: Neuroptera e Labiidae: Dermaptera e parasitoides, como os microhimenópteros. Foram encontrados três tipos de larvas, sendo classificadas como curculioniforme as mais numerosas, apresentando um total de 57 espécimes coletados.Entomofaunistic survey of artropods in naturally colored cotton fiber in Ipanguaçu-RNAbstract: The objective of this research was to identify the diversity of arthropods associated with cotton crop Gossypium hirsutum L. with colorful plume, it was conducted entomofaunístico survey in Ipanguaçu-RN in production areas. The experimental area was composed of 15 cotton

  17. Saproxylic Hemiptera Habitat Associations

    Science.gov (United States)

    Michael D. Ulyshen; James L. Hanula; Robert L. Blinn; Gene. Kritsky

    2012-01-01

    Understanding the habitat requirements of organisms associated with dead wood is important in order to conserve them in managed forests. Unfortunately, many of the less diverse saproxylic taxa, including Hemiptera, remain largely unstudied. An effort to rear insects from dead wood taken from two forest types (an upland pine-dominated and a bottomland mixed hardwood),...

  18. Efeito da cobertura vegetal sobre a pérola-da-terra (Hemiptera: Margarodidae na cultura da videira = Effect of cover crops on brazilian ground pearl (Hemiptera: Margarodidae in vineyards

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    Marcos Botton

    2010-10-01

    Full Text Available O uso da cobertura vegetal em vinhedos é uma prática empregada paraminimizar a erosão e melhorar as qualidades químicas e físicas do solo. Neste trabalho, foi avaliado o efeito de coberturas vegetais sobre a população da pérola-da-terra Eurhizococcus brasiliensis (Hemiptera: Margarodidae na cultura da videira. No primeiro experimento, o vinhedo foi mantido sem cobertura vegetal por meio da aplicação trimestral do herbicida glifosato comparado com o uso de vegetação espontânea, durante o ano, de vegetação espontânea, no verão, e de aveia preta no inverno. No segundo experimento foi avaliado o efeito da mucuna-preta (Stizolobium aterrimum cultivada no vinhedo durante o verão comparado com a vegetação espontânea. No primeiro experimento, a população da pérolada-terra nas raízes de plantas de videira foi maior em áreas mantidas sem cobertura vegetal emostrou-se semelhante em áreas onde se manteve a vegetação espontânea, ao longo do ano, e com aveia preta no inverno e vegetação espontânea no verão. A infestação das plantas de videira em áreas onde foi empregada a mucuna-preta durante o verão foi equivalente à da vegetação espontânea. S. aterrimum foi registrada pela primeira vez como hospedeira de E. brasiliensis. The use of cover crops is an important strategy to reduce erosion and improve chemical and physical soil properties. In this work, we evaluate the effect of cover crops to reduce Brazilian ground pearl Eurhizococcus brasiliensis (Hemiptera: Margarodidae infestation in vineyards. In the first experiment, glyphosate was sprayed each three months to avoid cover crops. This treatment was compared with naturally occurring vegetation during the year and the use of Avena sativa in the winter. In a second experiment, Stizolobium aterrimum was cultivated during the summer compared with naturally occurringvegetation. Brazilian ground pearl population was higher in glyphosate sprayed areas than where cover

  19. (Neuroptera: Chrysopidae fed with Aleurocanthus woglumi (Hemiptera: Aleyrodidae

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    Robério de Oliveira

    2016-02-01

    Full Text Available This study evaluated the development and reproduction of Ceraeochrysa cubana when fed the citrus blackfly Aleurocanthus woglumi. The study was carried out at the Laboratory of Entomology, Department of Plant and Environmental Sciences, Federal University of Paraíba – Areia/PB. It used a completely randomized design. The study was conducted in climate chambers, regulated to 26 ± 2°C and a relative humidity of 70 ± 10% with a 12 h photoperiod. The treatments were as follows: eggs, nymphs of only the 1st, 2nd, 3rd, or 4th instars, and nymphs of multiple instars (1st, 2nd, 3rd, and 4th instars of A. woglumi, with eggs of Sitotroga cerealella as a control. The 1st instar of C. cubana lasted 5.8 to 10.7 days, the 2nd instar lasted 6.0 to 13.3 days, and the 3rd instar lasted 8.2 to 18.5 days. The larvae of C. cubana did not survive when the food provided was only the eggs of A. woglumi, since the predator could not eat them. C. cubana, when provided nymphs of multiple instars (1st, 2nd, 3rd, and 4th instars of the citrus blackfly, had a shorter pre-pupal period and lower longevity than when consuming the eggs of S. cerealella, but the sex ratio and oviposition period were not affected. However, changes occurred in the pre-oviposition period, the total number of eggs, and the number of viable eggs.

  20. Sobre o condicionamento alimentar na cochonilha-branca, Planococcus citri (Risso (Hemiptera: pseudococcidae Regarding to host conditioning in citrus mealybug Planococcus citri (Risso (Hemiptera: pseudococcidae

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    Lenira Viana Costa Santa-Cecília

    2013-03-01

    Full Text Available Estudos do comportamento alimentar mediante a técnica de "Electrical Penetration Graphs" (EPG das cochonilhas-farinhentas (Pseudococcidae provenientes de um hospedeiro de criação alternativo têm mostrado que esses insetos não atingem ou demoram cerca de 9 horas para alcançar a fase floemática. Por outro lado, aqueles provenientes do hospedeiro-fonte atingem a fase floemática mais rapidamente e apresentam maior frequência de alimentação nos vasos crivados. Esses resultados indicam a presença do fenômeno de condicionamento alimentar, ainda não demonstrado em cochonilhas. Assim, o presente trabalho teve como objetivo determinar a existência desse fenômeno em Planococcus citri (Risso (Hemiptera: Pseudococcidae. Foram realizados testes de livre escolha, monitoramento eletrônico (EPG e estudos de alguns parâmetros biológicos. Em todos os experimentos, o cafeeiro (Coffea arabica L., os citros (Citrus sinensis L. e abóbora (Cucurbita maxima L. foram utilizados como substratos de criação (fonte da cochonilha, sendo os tratamentos constituídos pela combinação entre os hospedeiros-fonte e os hospedeiros receptores (café e citros. O teste de escolha entre cafeeiro e citros nas primeiras 72 horas mostrou que as cochonilhas criadas em cafeeiro apresentaram preferência pelo cafeeiro; aquelas originadas dos citros mostraram uma tendência, embora não significativa, em selecionar os citros em relação ao cafeeiro e aquelas criadas em abóbora não mostraram preferência por nenhum dos hospedeiros. Os estudos do comportamento alimentar mediante o monitoramento eletrônico (EPG mostraram que a fase floemática, considerada como a fase de aceitação do hospedeiro, foi mais frequente em cafeeiro, seja com cochonilhas oriundas deste substrato, seja de citros. Aqueles insetos mantidos em abóbora e transferidos para o cafeeiro ou citros apresentaram excepcionalmente ou não apresentaram nenhuma fase floemática, respectivamente. A

  1. First report on the whitefly, Aleurodicus pseudugesii on the coconut palm, Cocos nucifera in Brazil.

    Science.gov (United States)

    de Omena, Rose Paula Mendonça; Guzzo, Elio Cesar; Ferreira, Joana Maria Santos; de Mendonça, Fernando Antônio Cavalcante; de Lima, Aurino Florencio; Racca-Filho, Francisco; Santana, Antônio Euzébio Goulart

    2012-01-01

    The coconut palm, Cocos nucifera L. (Arecales: Arecaceae), is currently grown extensively throughout the intertropical zones of the world, including Brazil, where it constitutes an important source of income for growers. Although whiteflies are not normally considered coconut pests, these insects can damage crops directly by sucking the sap, which weakens the plant; indirect damage may be caused by sooty mold formation over the excreted honeydew and by the transmission of pathogens. Whiteflies have infested coconut plants in the northeastern, northern, and southeastern regions of Brazil. Infested materials were collected and the causative insect was identified as Aleurodicus pseudugesii Martin (Hemiptera: Aleyrodidae). This is the first report of A. pseudugesii in Brazil as a pest of the coconut palm.

  2. Primeiro registro de Guyalna cuta em pinhão-manso no estado de Mato Grosso do Sul

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    Harley Nonato de Oliveira

    2012-01-01

    Full Text Available Trata-se do primeiro registro de ocorrência de Guyalna cuta (Walker (Hemiptera: Cicadidae em pinhão-manso, Jatropha curcas L. (Euphorbiaceae no Estado de Mato Grosso do Sul. Adultos desse inseto foram observados na parte aérea da planta, durante novembro e dezembro de 2008 e de 2009. Em Mato Grosso do Sul, existem plantios comerciais e experimentais do pinhão-manso, tornando-se necessários estudos relacionados a este inseto, a fim de prevenir eventuais danos à cultura.

  3. Rediscovering digitules in Aphidomorpha and the question of homology among Sternorrhyncha (Insecta, Hemiptera

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    Mark A. Metz

    2017-07-01

    Full Text Available We explore and expand on the morphological term digitule. The term was originally proposed for toe-like setae on a species of Phylloxera Boyer de Fonscolombe, 1834 (Hemiptera, Sternorrhyncha, Aphidomorpha by Henry Shimer, an American naturalist. While it is standard terminology in scale systematics (Hemiptera, Sternorrhyncha, Coccidomorpha, the term digitule was ignored by aphid specialists despite being the original taxon for which the term was described. Similar setae occur on many arthropod groups, so the homology is poorly understood even within any superfamily of Hemiptera. We provide the etymology of the term, a proposed explanation for why it was used among scale taxonomists and not aphid taxonomists, and discuss briefly options to progress beyond the confusion between terminology for morphology and homology in Sternorrhyncha.

  4. Occurrence of Quesada gigas on Schizolobium amazonicum trees in Maranhão and Pará States, Brazil Ocorrência de Quesada gigas em Schizolobium amazonicum em municípios do Maranhão e do Pará

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    José Cola Zanuncio

    2004-09-01

    Full Text Available An infestation of Quesada gigas Olivier (Hemiptera: Cicadidae on "paricá" Schizolobium amazonicum (Huber Ducken (Fabales: Fabaceae was reported in the Municipalities of Itinga, Maranhão State and Paragominas, Pará State. Nymphs of this insect on roots and adults and exuvias on trunks of the plant were observed. Exit holes of nymphs in the soil were also observed. The occurrence of Q. gigas on S. amazonicum shows the damage potential of this species and the necessity of developing integrated management programs for species of this group, specially in reforested areas with "paricá".Há relatos sobre a infestação de Quesada gigas Olivier (Hemiptera: Cicadidae em paricá Schizolobium amazonicum (Huber Ducken (Fabales: Fabaceae nos municípios de Itinga, Estado do Maranhão e Paragominas, Estado do Pará. Observaram-se ninfas nas raízes e adultos e exúvias nos troncos da planta. Orifícios no solo, de saída de ninfas móveis dessa cigarra também foram verificados. A ocorrência de Q. gigas em S. amazonicum mostra o potencial de danos da espécie e a necessidade de se desenvolver programas de manejo integrado de espécies desse grupo, principalmente Q. gigas, em áreas reflorestadas com paricá.

  5. Host Range Specificity of Scymnus camptodromus (Coleoptera: Coccinellidae), A Predator of Hemlock Woolly Adelgid (Hemiptera: Adelgidae).

    Science.gov (United States)

    Limbu, Samita; Cassidy, Katie; Keena, Melody; Tobin, Patrick; Hoover, Kelli

    2016-02-01

    Scymnus (Neopullus) camptodromus Yu and Liu (Coleoptera: Coccinellidae) was brought to the United States from China as a potential biological control agent for hemlock woolly adelgid (Adelges tsugae Annand) (Hemiptera: Adelgidae). Scymnus camptodromus phenology is closely synchronized with that of A. tsugae and has several characteristics of a promising biological control agent. As a prerequisite to field release, S. camptodromus was evaluated for potential nontarget impacts. In host range studies, the predator was given the choice of sympatric adelgid and nonadelgid prey items. Nontarget testing showed that S. camptodromus will feed to some degree on other adelgid species, but highly prefers A. tsugae. We also evaluated larval development of S. camptodromus on pine bark adelgid (Pineus strobi (Hartig)) (Hemiptera: Adelgidae) and larch adelgid (Adelges laricis Vallot) (Hemiptera: Adelgidae); a small proportion of predator larvae was able to develop to adulthood on P. strobi or A. laricis alone. Scymnus camptodromus showed no interest in feeding on woolly alder aphid (Paraprociphilus tessellatus Fitch) (Hemiptera: Aphididae) or woolly apple aphid (Eriosoma lanigerum (Hausmann)) (Hemiptera: Aphididae), and minimal interest in cotton aphid (Aphis gossypii Glover) (Hemiptera: Aphididae) in choice and no-choice experiments. Scymnus camptodromus females did not oviposit on any host material other than A. tsugae-infested hemlock. Under the circumstances of the study, S. camptodromus appears to be a specific predator of A. tsugae, with minimal risk to nontarget species. Although the predator can develop on P. strobi, the likelihood that S. camptodromus would oviposit on pine hosts of this adelgid is small.

  6. Efeitos da temperatura e do alimento no desenvolvimento de Dysdercus maurus Distant (Hemiptera, Pyrrhocoridae Effects of temperature and food on the development of Dysdercus maurus Distant (Hemiptera, Pyrrhocoridae

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    Fábio Souto Almeida

    2007-12-01

    Full Text Available Dysdercus maurus Distant, 1901 (Hemiptera, Pyrrhocoridae é uma importante praga de Gossypium spp. (algodoeiro, Citrus Sinensis Osbeck (Rutaceae (laranjeira e Citrus reticulata (Rutaceae (tangerineira, além de sementes de Chorisia speciosa St. Hil. (paineira. Este trabalho objetivou avaliar os efeitos da temperatura e do alimento no desenvolvimento de D. maurus. Foram realizados oito tratamentos, seis em que os percevejos foram alimentados com sementes de paineira e mantidos a 15, 18, 20, 25 e 30 ± 1ºC, UR 80 ± 3% e fotofase de 12 h ou em condições ambientais de laboratório (23,5 ± 2,6ºC, UR 73,3 ± 9,9 %, e dois em que foram alimentados com sementes de algodão variedade IAC-22 e mantidos a 25 e 30ºC. Em todos os tratamentos foram observados cinco estágios imaturos. O aumento da temperatura proporcionou diminuição do tempo de desenvolvimento. A temperatura de 15ºC foi letal para ovos e ninfas de D. maurus. A menor mortalidade de ninfas ocorreu quando os percevejos foram alimentados com sementes de algodão a 25ºC (24,07%. A menor temperatura base (Tb foi obtida para o 1º ínstar (11,54ºC e a maior para o 2º ínstar (15,33ºC. As fêmeas de D. maurus necessitam de maior quantidade de graus-dias (329,93 graus-dias que os machos (300,49 graus-dias para atingir o estádio adulto.Dysdercus maurus Distant, 1901 (Hemiptera, Pyrrhocoridae is an important pest on Gossypium spp. (cotton tree, Citrus sinensis Osbeck (Rutaceae and Citrus reticulata Blanco (Rutaceae crops. This insect also feeds on seeds of Chorisia speciosa St. Hil. (Bombacaceae. This work aimed to evaluate the effects of temperature and food on the development of D. maurus. Eight treatments were carried out, in six of them bugs were fed with seeds of C. speciosa and kept at 15, 18, 20, 25 and 30 ± 1ºC, 80 ± 3% RH and 12h photophase or in laboratory conditions (23.5 ± 2.6ºC, 73.3 ± 9.9 % RH, and in the other two treatments bugs were fed with seeds of cotton variety

  7. Mortalidade de Coccus viridis (Hemiptera: Coccidae por Lecanicillium spp. em diferentes órgãos de Coffea arabica

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    Flávio Lemes Fernandes

    2009-04-01

    Full Text Available Objetivou-se neste trabalho avaliar a mortalidade de Coccus viridis por Lecanicillium spp. em folhas e ramos de Coffea arabica. O trabalho foi realizado na casa de vegetação na Universidade Federal de Viçosa, entre fevereiro de 2005 a junho de 2006. Foram utilizadas sementes da variedade Catuaí vermelho de café (C. arabica. A solução nutritiva utilizada foi composta por 3,0 mmol/L de N, 9,0 mmol/L de K, 1,0 mmol/L de P, 4,0 mmol/L de Ca, 2,1 mmol/L de S, 46 µmol/L de B, 0,3 µmol/L de Cu, 60,0 µmol/L de Fe, 2,0 µmol/L de Mg, 0,5 µmol/L de Mo e 36,0 µmol/L de Mn. As cochonilhas e o fungo foram coletados de folhas de café em lavouras comerciais de Viçosa, MG e foram mantidos em gaiolas em Casa de Vegetação. As plantas foram infestadas com oito meses de idade. A infestação foi realizada por contato de C. viridis infectadas com o fungo sobre as cochonilhas sadias. As densidades de C. viridis viva e morta nas folhas e nos ramos foram avaliadas aos 0, 15, 30, 35, 45, 60 e 75 dias após a infestação. Usou-se análise de variância, teste de média de Skott-Knott e análise de regressão linear simples a p<0,05. O fungo Lecanicillium spp. causou mortalidade em todos os estádios de C. viridis. De forma geral, a maior mortalidade ocorreu no terceiro ínstar. Além disso, o fungo causou maior mortalidade nesse inseto praga na face abaxial da folha de café. Mortality of Coccus viridis (Hemiptera: Coccidae by Lecanicillium spp. in differents Coffea arabica organs in greenhouseAbstract. The objective of this work was to evaluate the mortality of Coccus viridis by Lecanicillium spp. on faces of the leaves, and branches of Coffea arabica. This work was conducted in greenhouse at UFV. The experiment was carried out from February 2005 to June 2006. Seeds of the lineage IAC 15 from the variety “Catuaí Vermelho” coffee (C. arabica were used. The nutritive solution utilized was composed for 3. 0 mmol/L of N, 9. 0 mmol/L of K, 1. 0 mmol

  8. Corpos estranhos animados em otorrinolaringologia Strange animated bodies in othorinolaringology

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    Ricardo R. Figueiredo

    2002-10-01

    Full Text Available Forma de estudo: Clínico retrospectivo. Material e método: Foram reportados 56 casos de corpos estranhos animados em orelhas (55 insetos e 1 aracnídeo e 1 caso (inseto em fossas nasais. O material foi coletado no setor de Emergência do serviço de ORL do Hospital Municipal Souza Aguiar, no centro do Rio de Janeiro, entre os anos de 1998 e 2000, e identificado por zoólogos do Museu Nacional, Rio de Janeiro. A maior parte dos casos ocorreu em Nova Iguaçu e Campo Grande, sendo analisados os quadros clínicos e as complicações ocorridas. Resultado: Os insetos são: 30,35% Blattaria (baratas; 25% Diptera (moscas e mosquitos; 12,5% Lepidoptera (borboletas e mariposas; 10,7% Coleoptera (besouros; 7,15% Hemiptera (percevejos, cigarras, afídeos, etc., 5,35% Hymenoptera (vespas, abelhas, formigas, marimbondos e 5,31 % outros.Study design: Clinical retrospective. Material and method: Fufty-six cases of animated foreign bodies collected inside human ears (55 insects and 1 arachnid and one case collected in nasal fossae (insect were reported. The material was collected in the Emergency sector of Souza Aguiar Hospital, in Rio de Janeiro, between 1998 and 2000, and was identified by zoologists of Museu Nacional, Rio de Janeiro. Most of the cases had occurred in Nova Iguaçu and Campo Grande, suburbs of Rio de Janeiro. Clinical features and complications were analyzed. Results: The recorded insects are: 30,35% Blattaria (cockroaches; 25% Diptera (flies and mosquitos; 12,5% Lepidoptera (butterflies and moths; 10,7% Coleoptera (beetles; 7,15% Hemiptera (bugs, cicads, aphids, etc., 5,35% Hymenoptera (wasps, bees, ants, and sawflies and 5,31 % others.

  9. Indutores de resistência à mosca-branca Bemisia tabaci Biótipo B (GENN., 1889 (Hemiptera: Aleyrodidae em soja Resistance inducers to the whitefly Bemisia tabaci Biotype B (GENN., 1889 (Hemiptera: Aleyrodidae in soybeans

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    Jair Campos Moraes

    2009-10-01

    Full Text Available Objetivou-se, neste trabalho, avaliar o efeito da aplicação de indutores na resistência de duas cultivares de soja à mosca-branca Bemisia tabaci biótipo B. O experimento foi conduzido no Departamento de Entomologia - UFLA. As sementes de soja, cultivar IAC-19 e MONSOY-8001, foram previamente tratadas com o fungicida captan. Foram testados os seguintes tratamentos: T1- irrigação no solo, ao redor das plantas de cada vaso, de 250 mL de solução de ácido silícico a 1%; T2- pulverização das plantas, até o ponto de escorrimento, com solução a 0,3% de acibenzolar-s-methyl; T3- pulverização das plantas, até o ponto de escorrimento, com calda a 0,24% de pyraclostrobin+epoxiconazole e T4- testemunha. As plantas foram infestadas com 100 adultos da mosca-branca por vaso que liberados oito dias após a aplicação dos tratamentos. Avaliaram-se o número de ovos na face abaxial de cada folha, que foi marcada para posterior avaliação de ninfas; teores de taninos e de lignina; peso seco das raízes e da parte aérea. Não houve diferença para número de ovos e ninfas entre as cultivares de soja, porém, a viabilidade média de B. tabaci foi menor a cultivar IAC-19. O cultivar de soja IAC-19 demonstrou moderada resistência ao biótipo B de B. tabaci. A aplicação de silício ou acibenzolar-s-methyl induz um aumento no teor de lignina na cultivar de soja IAC-19.The objective of this paper was to evaluate the effect of the application of inducers on the resistance of two soybean cultivars to the whitefly B. tabaci Biotype B. The experiment was carried out at the Entomology Department - UFLA. The soybean seeds of cultivars IAC-19 and MONSOY-8001 were previously treated with Captan fungicide. The following treatments were tested: T1- irrigating the soil around the plants of each pot with 250 mL of solution of 1% silicic acid; T2- spraying of the plants, to the 'point of dripping', with a solution of 0.3% acibenzolar-s-methyl; T3- spraying of the plants, to the 'point of dripping', with a solution of 0.24% pyraclostrobin+epoxiconazole, and T4- control. The plants were infested with 100 adult whiteflies that were released eight days after applying the treatments. The number of eggs on the abaxial face of each leaf, which was marked for further evaluation of nymphs, tannin and lignin contents, dry weight of the aerial parts and roots, was evaluated. There was no difference for the number of eggs and nymphs among the soybean cultivars. Nevertheless, the average survival rate of B. tabaci was lower on cultivar IAC-19. The application of silicon or acibenzolar-s-methyl induces an increase in lignin content in the soybean cultivar IAC-19.

  10. Preliminary Observations on Zelus obscuridorsis (Stål (Hemiptera: Reduviidae as Predator of the Corn Leafhopper (Hemiptera: Cicadellidae in Argentina

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    Eduardo G. Virla

    2015-06-01

    Full Text Available The corn leafhopper Dalbulus maidis (Hemiptera: Cicadellidae, is an important corn pest in most of tropical and subtropical America. This leafhopper has a rich natural enemy complex of which parasitoids and pathogens are the most studied; knowledge on its predators is limited. We noted the presence of the native assassin bug Zelus obscuridorsis (Hemiptera: Reduviidae predating diverse motile insects, including the corn leafhopper, on corn plants cultivated in household vegetable gardens in San Miguel de Tucumán (Argentina; in order to verify its predatory actions, we exposed lab-bred individuals of D. maidis to adults of Z. obscuridorsis. The predators were starved for 24 h before trials in which the corn leafhopper in different developmental stages were exposed. Zelus obscuridorsis is highly skilled in catching specimens in motion, but it was not able to prey on eggs. The predator was capable to catch and prey on nymphs and adults.

  11. Sobre o condicionamento alimentar na cochonilha-branca, Planococcus citri (Risso (Hemiptera: pseudococcidae

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    Lenira Viana Costa Santa-Cecília

    2013-03-01

    Full Text Available Estudos do comportamento alimentar mediante a técnica de "Electrical Penetration Graphs" (EPG das cochonilhas-farinhentas (Pseudococcidae provenientes de um hospedeiro de criação alternativo têm mostrado que esses insetos não atingem ou demoram cerca de 9 horas para alcançar a fase floemática. Por outro lado, aqueles provenientes do hospedeiro-fonte atingem a fase floemática mais rapidamente e apresentam maior frequência de alimentação nos vasos crivados. Esses resultados indicam a presença do fenômeno de condicionamento alimentar, ainda não demonstrado em cochonilhas. Assim, o presente trabalho teve como objetivo determinar a existência desse fenômeno em Planococcus citri (Risso (Hemiptera: Pseudococcidae. Foram realizados testes de livre escolha, monitoramento eletrônico (EPG e estudos de alguns parâmetros biológicos. Em todos os experimentos, o cafeeiro (Coffea arabica L., os citros (Citrus sinensis L. e abóbora (Cucurbita maxima L. foram utilizados como substratos de criação (fonte da cochonilha, sendo os tratamentos constituídos pela combinação entre os hospedeiros-fonte e os hospedeiros receptores (café e citros. O teste de escolha entre cafeeiro e citros nas primeiras 72 horas mostrou que as cochonilhas criadas em cafeeiro apresentaram preferência pelo cafeeiro; aquelas originadas dos citros mostraram uma tendência, embora não significativa, em selecionar os citros em relação ao cafeeiro e aquelas criadas em abóbora não mostraram preferência por nenhum dos hospedeiros. Os estudos do comportamento alimentar mediante o monitoramento eletrônico (EPG mostraram que a fase floemática, considerada como a fase de aceitação do hospedeiro, foi mais frequente em cafeeiro, seja com cochonilhas oriundas deste substrato, seja de citros. Aqueles insetos mantidos em abóbora e transferidos para o cafeeiro ou citros apresentaram excepcionalmente ou não apresentaram nenhuma fase floemática, respectivamente. A

  12. Comparative efficacy of some insecticides against cotton whitefly, bemisia tabaci (gennadius) (homoptera: aleyrodidae) under natural field conditions

    International Nuclear Information System (INIS)

    Nadeem, M.K.; Hasnain, M.; Ahmed, S.; Ashfaq, M.

    2011-01-01

    Comparative efficacy of five commonly used insecticides viz., acetamiprid, buprofezin, diafenthiuron, imidacloprid and endosulfan against nymph and adult population of cotton whitefly, Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae) under natural field conditions has been studied. Results showed that buprofezin was the most effective insecticide against nymph population of whitefly among the tested insecticides where nymphal population of B. tabaci was 0.2/leaf after 24h spray as compared to 1.9/leaf in control. Acetamiprid was the most effective against adult population of whitefly (0.3 to 1.3/leaf post 72 h spray, as compared to control with 6.9 to 8.2/leaf) followed by diafenthiuron and imidaclopirid. whereas, endosulfan was found to be the least effective on both populations as adult and nymph of whitefly. From the tested insecticides, acetamaprid gave effective control of both nymph and adult population of B. tabaci. (author)

  13. Custos ecofisiológicos do cuidado maternal em Antiteuchus sepulcralis (Fabricius) (Hemiptera: Pentatomidae)

    OpenAIRE

    Santos, Adão V.; Albuquerque, Gilberto S.

    2001-01-01

    Para estudar as características biológicas relacionadas com o cuidado maternal em Antiteuchus sepulcralis (Fabricius), foram feitas observações em 28 fêmeas, com suas respectivas posturas, criadas na planta hospedeira Hibiscus pernambucensis Arruda (Malvaceae). As posturas foram individualizadas em gaiolas de filó e verificadas diariamente no período de setembro/97 a maio/98, à temperatura média de 27,7°C. Para observar as estratégias de defesa exibidas pela fêmea, colocaram-se um ou mais ind...

  14. Essential oils as fumigants for bed bugs (Hemiptera: Cimicidae)

    Science.gov (United States)

    In Petri dish assays, fumigation of a pyrethroid-susceptible strain of bed bugs Cimex lectularius L. (Hemiptera: Cimicidae) with various essential oils resulted in mortality that approached or equaled 100%, after 5 days. However, when bed bugs were exposed to the same essential oils in sealed, comme...

  15. An experimental test of rainfall as a control agent of Glycaspis brimblecombei Moore (Hemiptera, Psyllidae on seedlings of Eucalyptus camaldulensis Dehn (Myrtaceae

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    Karla N. Oliveira

    2012-03-01

    Full Text Available An experimental test of rainfall as a control agent of Glycaspis brimblecombei Moore (Hemiptera, Psyllidae on seedlings of Eucalyptus camaldulensis Dehn (Myrtaceae. Glycaspis brimblecombei is one the greatest threats to eucalyptus plantations in Brazil. The effects of rainfall to reduce the abundance of lerp of Glycaspis brimblecombei on experimentally infested seedlings of Eucalyptus camaldulensis were assessed. The number of lerps on the adaxial and abaxial surfaces of every leaf of 60 seedlings was recorded, before and after submission to the following treatments: "artificial rain", "leaf wetting" and control. A drastic reduction in lerp abundance per plant was observed after the treatments "leaf wetting" and artificial rain (F = 53.630; p Teste experimental da chuva como agente de controle de Glycaspis brimblecombei Moore (Hemiptera, Psyllidae em mudas de Eucalyptus camaldulensis Dehn (Myrtaceae. Glycaspis brimblecombei é uma das maiores ameaças das plantações de eucalipto do Brasil. Foram avaliados os efeitos da água na redução da abundância de conchas desse inseto em mudas de Eucalyptus camaldulensis infestadas experimentalmente. Foi quantificado o número de conchas nas superfícies adaxial e abaxial de todas as folhas de 60 mudas, antes e após a aplicação dos seguintes tratamentos: "chuva artificial", "molhamento das folhas" e controle. Foi observada uma drástica redução na abundância de conchas nos tratamentos "chuva artificial" e "molhamento das folhas" (F = 53,630; p < 0,001, o que não ocorreu para o tratamento controle ao longo do experimento (F = 1,450; p = 0,232. Ao final do experimento, a abundância de conchas foi significativamente menor no tratamento "chuva artificial" e "molhamento das folhas" do que no tratamento controle. Dessa forma, dois dias de chuva mostraram ser eficientes para diminuir mais que 50% da população de conchas, com quase 100% de eficiência após 5 dias de experimento. Nossos resultados

  16. Medición indirecta de la tasa de consumo de adultos e inmaduros de Trialeurodes vaporariorum (Hemiptera: Aleyrodidae sobre fríjol

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    Luisa Fernanda Suarez Gonzalez

    2015-09-01

    Full Text Available La biología y ecología de la mosca blanca de los invernaderos, Trialeurodes vaporariorum (Westwood (Hemiptera: Aleyroridae, se encuentra ampliamente documentada. Sin embargo, hasta el momento no hay reportes acerca de la tasa de consumo de alimento por este fitófago, un parámetro necesario para modelos de simulación. Por tal motivo, el objetivo de esta investigación fue la medición indirecta del consumo de alimento por el adulto e instares inmaduros de T. vaporariorum basado en metodologías encontradas en la literatura científica. La estimación de la tasa de consumo de alimento por adultos se realizó bajo condiciones de laboratorio, utilizando como unidad experimental una planta de fríjol de dos semanas de edad. El experimento consistió de dos tratamientos: (T1 plantas libres de cualquier fitófago y (T2 plantas sometidas a infestaciones con T. vaporariorum, cada tratamiento con 20 repeticiones y una réplica en el tiempo de todo el experimento. Se registró el peso fresco diariamente en ambos tratamientos. Para el caso de los inmaduros, la unidad experimental consistió en una hoja cotiledónea de fríjol, infestada con una población determinada de inmaduros de cada instar de mosca blanca teniendo así cinco tratamientos (Instar I, II, III, IV y Control cada uno con 17 repeticiones. Se registró diariamente el peso fresco de las hojas. Se obtuvo un consumo promedio de alimento por el adulto de 0,277 ± 0,0042 mg.dia-1. Para los dos primeros instares ninfales se estimó una tasa de consumo de 0,052 mg.dia-1, en el tercer instar de 0,14 mg.dia-1, y para el cuarto instar de 0,19 mg.dia-1. Un adulto de mosca blanca puede consumir en un día seis veces su peso y un inmaduro ocho veces, gracias a su rápido metabolismo y la constante excreción de mielecilla.

  17. Verde plant bug, Creontiades signatus (Hemiptera: Miridae) effects of insect density and bloom period of infestation on cotton damage and yield

    Science.gov (United States)

    The verde plant bug, Creontiades signatus Distant (Hemiptera: Miridae), has emerged as a threat to cotton in South Texas, causing boll damage similar to boll-feeding stink bugs (Hemiptera: Pentatomidae). Verde plant bugs were released into caged cotton for a one-week period to characterize the effec...

  18. Analysis of the genetic diversity in Metopolophium dirhodum (Walker (Hemiptera, Aphididae by RAPD markers Análise da diversidade genética de Metopolophium dirhodum (Walker (Hemiptera, Aphididae por meio de marcadores RAPD

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    Marcelo Lopes-da-Silva

    2007-03-01

    Full Text Available The emergence of host-races within aphids may constitute an obstacle to pest management by means of plant resistance. There are examples of host-races within cereals aphids, but their occurrence in Rose Grain Aphid, Metopolophium dirhodum (Walker, 1849, has not been reported yet. In this work, RAPD markers were used to assess effects of the hosts and geographic distance on the genetic diversity of M. dirhodum lineages. Twenty-three clones were collected on oats and wheat in twelve localitites of southern Brazil. From twenty-seven primers tested, only four primers showed polymorphisms. Fourteen different genotypes were revealed by cluster analysis. Five genotypes were collected only on wheat; seven only on oats and two were collected in both hosts. Genetic and geographical distances among all clonal lineages were not correlated. Analysis of molecular variance showed that some molecular markers are not randomly distributed among clonal lineages collected on oats and on wheat. These results suggest the existence of host-races within M. dirhodum, which should be further investigated using a combination of ecological and genetic data.A emergência de raças hospedeiro-especialistas em afídeos pode constituir um obstáculo ao manejo de pragas por meio de plantas resistentes. Existem exemplos de raças hospedeiro-especialistas em afídeos de cereais, embora a ocorrência de raça hospedeiro-especialista no pulgão-verde-pálido-do-trigo Metopolophium dirhodum (Walker, 1849 (Hemiptera, Aphididae não tenha sido relatada ainda. Marcadores RAPD foram utilizados para avaliar os efeitos da distância geográfica e do hospedeiro sobre a diversidade genética de linhas clonais de M. dirhodum. Vinte e três clones foram coletados em aveia e trigo em doze localidades do sul do Brasil. De vinte e sete iniciadores usados para a análise, apenas quatro iniciadores mostraram polimorfismos. A análise de agrupamento por similaridade genética revelou haver quatorze

  19. Development of DNA barcodes of genus Lygus Hahn (Hemiptera: Miridae)

    Science.gov (United States)

    The genus Lygus (Hemiptera: Miridae) is an important group of insects that contains 43 known species worldwide. Some species within this genus are important agricultural pests in North America. Annual economic impacts in cotton, Gossypium hirsutum L., from Lygus spp. due to yield losses and control ...

  20. Preparing soft-bodied arthropods for microscope examination: Whiteflies (Insecta: Hemiptera: Alyrodidae)

    Science.gov (United States)

    Proper identification of whiteflies (Hemiptera:Alyrodidae) requires preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare whitefly specimens on microscope slides for examination and identification. Steps ranging from collection, specimen...

  1. Preparing soft-bodied arthropods for microscope examination: Aphids (Insecta: Hemiptera: Aphididae)

    Science.gov (United States)

    Proper identification of aphids (Hemiptera: Aphididae) require preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare aphid specimens on microscope slides for examination and indentification. Steps ranging from collection, specimen clear...

  2. Catalog of the adelgids of the world (Hemiptera, Adelgidae)

    Science.gov (United States)

    Colin Favret; Nathan P. Havill; Gary L. Miller; Masakazu Sano; Benjamin Victor

    2015-01-01

    A taxonomic and nomenclatural Catalogue of the adelgids (Hemiptera: Adelgidae) is presented. Six family-group names are listed, five being synonyms of Adelgidae. Twenty-two genus-group names, of which nine are subjectively valid and in use, are presented with their type species, etymology, and grammatical gender. One hundred and six species-group names are listed, of...

  3. The complete mitochondrial genome sequence of Diaphorina citri (Hemiptera: Psyllidae)

    Science.gov (United States)

    The first complete mitochondrial genome (mitogenome) sequence of Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae), from Guangzhou, China is presented. The circular mitogenome is 14,996 bp in length with an A+T content of 74.5%, and contains 13 protein-coding genes (PCGs), 22 tRNA genes ...

  4. Occurrence and characterization of entomogen galls in plants from natural vegetation areas in Delfinópolis, MG, Brazil Ocorrência e caracterização de galhas entomógenas em plantas de áreas de vegetação natural em Delfinópolis, MG, Brasil

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    M. V. Urso-Guimarães

    2003-11-01

    Full Text Available In the present work we aimed to register the occurrence of galls, inductors, inquilines, and parasitoids in plants of three natural vegetation areas in Delfinópolis, MG, Brazil. Results obtained showed 22 types of galls collected from leaf, vein leaf, petioles, stem, and inflorescence of nineteen species belonging to fifteen distinct families. Concerning gall morphology, the following were collected: globoid, conicle, discoidal, fusiform, shell-shape, indefinite, and one substituition of an ovary by an immature. As principal inducers were found insects of the families Cecidomyiidae (Diptera, Psyllidae, and Diaspididae (Sternorrhyncha/Hemiptera. As parasitoids the most common are of the Chalcidoidea superfamily (Hymenoptera and, as occasional inquilines, Polyxenidae (Diplopoda and Psocodea (Psocoptera. The results of this study contribute to existing of knowledge host-plant diversity and gall-associated insects in rocky fields, cerrado, and gallery forests.Neste trabalho registramos a ocorrência de galhas, galhadores, inquilinos e parasitóides em plantas de três áreas de vegetação natural em Delfinópolis, MG, Brasil. Como resultado foram obtidas galhas coletadas em folhas, nervuras de folhas, pecíolos, ramos e inflorescências de quinze famílias distintas. Quanto à morfologia, foram coletadas galhas globóides, cônicas, discóides e fusiformes, em forma de concha, uma sem formato definido e uma substituição do ovário pelo imaturo. Como principais indutores foram obtidos insetos das famílias Cecidomyiidae (Diptera, Psyllidae e Diaspididae (Sternorrhyncha/Hemiptera. Os parasitóides são da superfamília Chalcidoidea (Hymenoptera e, como inquilinos ocasionais, Polyxenidae (Diplopoda e Psocodea (Psocoptera. Os resultados deste trabalho contribuem para aumento do conhecimento sobre a diversidade de plantas hospedeiras e insetos galhadores associados à vegetação de campo rupestre, cerrado e mata de galeria.

  5. Dinâmica populacional e parasitismo natural de Diaphorina citri Kuwayama (Hemiptera: Liviidae em pomares de citros em Sergipe

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    Adriano Farias

    2018-04-01

    Abstract. The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Sternorrhyncha: Liviidae, which is the vector of the Hunglongbing (HLB bacterium, has become a key citrus pest in Brazil. In addition to citrus, the ornamental plant known as Orange Jasmine, Murraya paniculata (L. Jack, also hosts the psyllid. The present work aimed at studying the population dynamics of the psyllid in citrus orchards and its parasitism in citrus and M. paniculata in the state of Sergipe, the fourth largest citrus-producing state in Brazil. The evaluations were performed fortnightly for eleven months in seven Pera Citrus sinensis (L. Osbeck orange orchards located in two municipalities of Sergipe state (Boquim, and Umbauba. The populations of eggs, nymphs and adults of D. citri were compared among all orchards and all developmental stages were related to the abiotic factors temperature, relative humidity and precipitation, in each locality. The psyllid was classified as an accessory species (not very abundant in the orchards of Sergipe, showing a higher population density in November, December and March. Precipitation was the only abiotic factor that contributed to the population increase of adults of the psyllid. High rates of parasitism (55% of psyllid nymphs by the exotic parasitoid Tamarixia radiata Waterston (Hymenoptera: Eulophidae were detected in M. paniculata plants. Based on the results, if the HLB bacterium is detected in Sergipe, shorter samplings should be performed in the spring and summer months aiming at vector management. In addition, T. radiata could be released inundatively in integrated vector management programs.

  6. “Candidatus Phytoplasma pyri” affects behavior of Cacopsylla pyricola (Hemiptera: Psyllidae)

    Science.gov (United States)

    Pear psylla, Cacopsylla pyricola (Hemiptera: Psyllidae), is a key pest of pear and is a vector of "Candidatus Phytoplasma pyri", the pathogen associated with pear decline disease. Although commercial pear trees are grafted to Phytoplasma-resistant rootstock, a recent report indicated that many C. p...

  7. Observations on some species of the genus Lyramorpha Westw. (Hemiptera Heteroptera)

    NARCIS (Netherlands)

    Schouteden, H.

    1908-01-01

    1. Lyramorpha pallida Westwood and L. rosea Westwood. In his »Catalogue of Hemiptera in the Collection of the Rev. W. F. Hope”, part I, London 1837, Westwood founded the genus Lyramorpha, with two species, L. rosea and L. pallida, both from New Holland.

  8. Spruce aphid (Elatobium abietinum Walker) (Hemiptera: Aphididae) [Chapter XXIV

    Science.gov (United States)

    Ann M. Lynch

    2014-01-01

    Elatobium abietinum Walker is a spruce-feeding aphid that in Europe is referred to as the green spruce aphid (Day et al., 1998a) (Fig. 1). However, in North America E. abietinum is known simply as the spruce aphid, while the common name "green spruce aphid" refers to a different species, Cinara fornacula Hottes (Hemiptera: Aphididae) (http://www.entsoc.org/...

  9. Use of biorational for the vegetable pest control in the north of Sinaloa

    Directory of Open Access Journals (Sweden)

    María Berenice González Maldonado

    2012-09-01

    Full Text Available In Sinaloa the vegetable and cucurbits production are important agricultural activities, so each year a high volume of chemicalinsecticides are applied to pest control that attack these crops. This paper present the main pests insects in the region, as wellas an analysis about effects of biorational insecticides on these pests. Was found that for control of Bemisia argentifolii Bellows & Perring (Hemiptera: Aleyrodidae is used Neem oil 0.2%., for kill nymphs of Bactericera cockerelli Sulc. (Homoptera: Psyllidae soursop Annona muricata L. (Annonales: Annonaceae at doses of 2500-5000 mg/L., for Liriomyza trifolii Burgess (Diptera: Agromyzidae neem seeds 2%., to Myzus persicae Sulzer (Hemiptera: Aphididae rapeseed oil at doses 920 g/L (2% v/v., to Frankliniella occidentalis Pergande (Thysanoptera: Thripidae spinosad (Conserve® 48-60 mg/L., and for Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae granular viruses (105 OBs/mL combined with neem (DalNeem TM emulsifiable oil and NeemAzal TM -T/S at doses of 8 mg/L, everyone. The use of these products and the dose depends on the type of pest and crop. In general these products cause insect mortality greater than 95%, besides having low toxicity on natural enemies, so that these can be used individually or in combination in integrated pest control schemes against vegetable pests, and also for disease vectors insects in the northern of Sinaloa.

  10. Rhizoecus colombiensis Ramos & Caballero, a new species of hypogeal mealybug (Hemiptera: Coccomor.

    Science.gov (United States)

    Ramos-Portilla, Andrea Amalia; Caballero, Alejandro

    2016-03-14

    A new species belonging to Rhizoecus Künckel d'Herculais (Hemiptera: Coccomorpha: Rhizoecidae) is described, with hosts and distribution data in the New World. A dichotomous and illustrated key for the twelve species of Rhizoecus recorded from Colombia is presented.

  11. Preparing soft-bodied arthropods for microscope examination: Soft Scales (Insecta: Hemiptera: Coccidae)

    Science.gov (United States)

    Proper identification of soft scales (Hemiptera:Coccidae) requires preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare soft scale specimens on microscope slides for examination and identification. Steps ranging from collection, speci...

  12. Spittlebug Cephisus siccifolius damaging eucalypt plants in the State of Bahia, Brazil Cigarrinha-de-espuma Cephisus siccifolius em plantio de eucalipto clonal no Estado da Bahia, Brasil

    Directory of Open Access Journals (Sweden)

    Genésio Tâmara Ribeiro

    2005-07-01

    Full Text Available Most common pests of eucalyptus plants in Brazil are leaf cutting ants and defoliating caterpillars. Other groups, eventually, feeding on eucalyptus include Heteroptera and cicadas. The objectives of this work were to identify and to describe attack symptoms of Cephisus siccifolius (Walker 1851 (Hemiptera: Aphrophoridae on eucalyptus trees in a stand of 25.77 hectares in the State of Bahia, Brazil, where 99.3% of them were attacked by this spittlebug. Damage was more severe on branches and leaves. Tree death was, relatively, low while those cut showed 4.7 colonies of this spittlebug with 21.9 nymphs per colony. Cultural control with the removal of trees attacked was recommended.Os insetos-praga mais comuns em plantios de eucalipto no Brasil são formigas cortadeiras e lagartas desfolhadoras. Eventualmente, outros insetos como percevejos e cigarrinhas podem danificar essas plantas. O objetivo deste trabalho foi identificar e descrever os sintomas de ataque, em um plantio de eucalipto de 25,77 hectares, no Estado da Bahia, onde 99,3% das árvores tinham sido atacadas pela cigarrinha-de-espuma Cephisus siccifolius (Walker 1851 (Hemiptera: Aphrophoridae. Os danos foram maiores em galhos e folhas. A mortalidade de árvores foi baixa, apesar de se observar 4,7 colônias de cigarrinhas por árvore, com 21,9 ninfas por colônia. O corte das árvores infestadas foi recomendado como controle.

  13. Instar- and stage-specific photoperiodic diapause response of Lygus hesperus (Hemiptera: Miridae)

    Science.gov (United States)

    The western tarnished plant bug (Lygus hesperus Knight)(Hemiptera:Miridae) is a polyphagous pest of numerous western crops. This pest overwinters in a relatively short duration adult diapause, but many details regarding diapause induction and maintenance remain unstudied. Instar-specific responses t...

  14. Mosca-Branca, Bemisia tabaci (Genn. (Hemiptera: Aleyrodidae em feijoeiro: Características gerais, bioecologia e métodos de controle

    Directory of Open Access Journals (Sweden)

    Anderson Silva

    2017-04-01

    Abstract. Common bean plants are infested by insects, which can ultimately affect the crop production before and after harvest, with estimated losses ranging from 33 to 86%. Among the insect pests infesting the common beans the whitefly Bemisia tabaci (Genn. stands out. This species cause direct injury by feeding on the plants and indirect injury by excreting sugary honeydew that is after colonized by the sooty mold. In addition, the most serious damage caused by B. tabaci is the transmission of virus diseases, especially the common bean golden mosaic, responsible for economic losses varying from 30 to 100%. This review aims at providing information on important aspects of B. tabaci including its geographical distribution, bioecology, population dynamics, host plants, and methods of pest control. We expect that this review can provide valuable subsidies for future studies on the whitefly in common beans.

  15. EFICIÊNCIA DO CONTROLE QUÍMICO SOBRE A MOSCA BRANCA Bemisia tabaci biótipo B (HEMIPTERA: ALEYRODIDAE EM MELOEIRO

    Directory of Open Access Journals (Sweden)

    Antônio Lindemberg Martins Mesquita

    2007-01-01

    efficient to control eggs, nymphs and adults of the whitefly, while the insect growth regulators buprofezin and pyriproxyfen did not control eggs and adults, but they controled nymphs, being pyriproxyfen more efficient than buprofezin.

  16. Temperature stress effects in Bemisia tabaci (Hemiptera: Aleyrodidae) type B whiteflies

    Science.gov (United States)

    Oxidative stress occurs in response to changes in the redox equilibiurm, which may be caused by increases in reactive oxygen species (ROS), a decrease in antioxidant protection or failure of cells to repair oxidative damage. ROS are either free radicals, reactive molecules containing oxygen atoms or...

  17. Preparing sternorrhynchous insects (Insecta: Hemiptera: Sternorrhyncha) for microscope examination: Hoyer’s mounting medium

    Science.gov (United States)

    Proper identification of aphids, scale insects, psyllids, and whitefles (Hemiptera: Sternorrhyncha) require preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare sternorrhynchous specimens on microscope slides for examination and identi...

  18. Preparing soft-bodied arthropods for arthropods for microscope examination: Mealybugs (Insects: Hemiptera: Pseudococcidae)

    Science.gov (United States)

    Proper identification of mealybugs (Hemiptera: Pseudococcidae) require preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare mealybug specimens on microscope slides for examination and identification. Steps ranging from collection, spec...

  19. Hemiptera Mitochondrial Control Region: New Sights into the Structural Organization, Phylogenetic Utility, and Roles of Tandem Repetitions of the Noncoding Segment

    Directory of Open Access Journals (Sweden)

    Kui Li

    2018-04-01

    Full Text Available As a major noncoding fragment, the control region (CR of mtDNA is responsible for the initiation of mitogenome transcription and replication. Several structural features of CR sequences have been reported in many insects. However, comprehensive analyses on the structural organization and phylogenetic utility, as well as the role of tandem replications (TRs on length variation, high A+T content, and shift of base skew of CR sequences are poorly investigated in hemipteran insects. In this study, we conducted a series of comparative analyses, using 116 samples covering all 11 infraorders of the five currently recognized monophyletic groups in the Hemiptera. Several structural elements (mononucleotide stretches containing conserved sequence blocks (CSBs, TRs, and GA-rich region were identified in the mitochondrial control region in hemipteran insects, without showing a consistent location. The presence and absence of certain specific structural elements in CR sequences show the various structural organizations of that segment among the five monophyletic groups, which indicates the diversification of the control region’s structural organization in Hemiptera. Among the many groups within Hemiptera, eight monophyletic groups and three consistent phylogenetic trees were recovered, using CSBs datasets by maximum likelihood and Bayesian methods, which suggests the possible utility of CR sequences for phylogenetic reconstruction in certain groups of Hemiptera. Statistical analyses showed that TRs may contribute to the length variation, high AT content, and the shift of base skewing of CR sequences toward high AT content in the Hemiptera. Our findings enrich the knowledge of structural organization, phylogenetic utility, and roles of tandem replication of hemipteran CR, and provide a possible framework for mitochondrial control region analyses in hemimetabolous insects.

  20. Scymnus camptodromus (Coleoptera: Coccinellidae) larval development and predation of hemlock woolly adelgid (Hemiptera: Adelgidae)

    Science.gov (United States)

    Samita Limbu; Melody A. Keena; David Long; Nancy Ostiguy; Kelli. Hoover

    2015-01-01

    Development time and prey consumption of Scymnus (Neopullus) camptodromus Yu and Liu (Coleoptera: Coccinellidae) larvae by instar, strain, and temperature were evaluated. S. camptodromus, a specialist predator of hemlock woolly adelgid Adelges tsugae (Annand) (Hemiptera:...

  1. New species of Braggia (Hemiptera: Aphididae) on buckwheat in western North America

    Science.gov (United States)

    K. S. Pike; G. Graf; R. G. Foottit; H. E. L. Maw; P. Stary; R. Hammon; D. G. Miller

    2009-01-01

    Species of Braggia Gillette and Palmer (Hemiptera: Aphididae: Aphidinae: Aphidini) feed on various buckwheat, Eriogonum Michx. (Polygonaceae), species in western North America. Two new species, Braggia columbiana Pike n. sp. from Washington and Oregon and Braggia longicauda Pike n. sp. from Washington, Oregon, and northern California, are proposed. Descriptions,...

  2. Gut content analysis of a phloem-feeding insect, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae)

    Science.gov (United States)

    Potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae) is a key pest of potato (Solanum tuberosum L., Solanales: Solanaceae) and a vector of "Candidatus Liberibacter solanacearum," the pathogen associated with zebra chip disease. In addition to its presence on cultivated crops, the p...

  3. Distribution and Abundance of Adelges tsugae (Hemiptera: Adelgidae) Within Hemlock Trees

    Science.gov (United States)

    S.V. Joseph; J.L. Hanula; S.K. Braman

    2011-01-01

    We studied the distribution of hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae), within hemlock trees for three summer (progrediens) and two winter (sistens) generations in northern Georgia. Eastern hemlock, Tsuga canadensis (L.) Carrie` re, trees were treated with 0, 10, or 25% of 1.5 g of imidacloprid per 2.5 cm of tree diameter at breast height...

  4. Levantamento da fauna de Triatominae (Hemiptera: Reduviidae em ambiente domiciliar e infecção natural por Trypanosomatidae no Estado de Mato Grosso do Sul Survey of Triatominae (Hemiptera: Reduviidae fauna in domestic environments and natural infection by Trypanosomatidae in the State of Mato Grosso do Sul

    Directory of Open Access Journals (Sweden)

    Paulo Silva de Almeida

    2008-08-01

    Full Text Available Entre 2000 a 2004, foi realizado levantamento da fauna de Triatominae (Hemiptera: Reduviidae e exame de infecção natural por Trypanosomatidae, no Estado de Mato Grosso do Sul. Um total de 13.671 espécimes foram capturados. Na análise faunística das espécies capturadas, Triatoma sordida foi caracterizada como muito abundante, muito freqüente, constante e dominante. Os índices de infecção natural para Trypanosoma cruzi apresentaram os valores de 3,2% para Panstrongylus geniculatus, 0,6% para Rhodnius neglectus e 0,1% para Triatoma sordida, apesar do Estado de Mato Grosso do Sul apresentar-se livre da transmissão vetorial endêmica.Between 2000 and 2004, a survey of Triatominae fauna (Hemiptera: Reduviidae and examination of natural infection caused by Trypanosomatidae in the State of Mato Grosso do Sul, Brazil, was conducted. A total of 13,671 specimens were collected. Through fauna analysis on the insects that were caught, Triatoma sordida was characterized as very abundant, very frequent, constant and dominant. The rates of natural infection with Trypanosoma cruzi were 3.2% for Panstrongylus geniculatus, 0.6% for Rhodnius neglectus and 0.1% for Triatoma sordida. Nevertheless, the State of Mato Grosso do Sul is free from endemic vector transmission.

  5. Effects of fertilizer and low rates of Imidacloprid on Adelges tsugae (Hemiptera: Adelgidae).

    Science.gov (United States)

    S. V. Joseph; James Hanula; S. K. Braman; F. J. Byrne

    2011-01-01

    Healthy hemlock trees, Tsuga canadensis (L.) Carriere, and hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae), populations should favor retention and population growth of adelgid predators such as Laricobius nigrinus Fender (Coleoptera: Derodontidae) and Sasajiscymnus tsugae (Sasaji&McClure) (Coleoptera: Coccinellidae). Eastern hemlock trees...

  6. Revisional notes on the genus Melucha (Hemiptera, Heteroptera, Coreidae

    Directory of Open Access Journals (Sweden)

    Harry Brailovsky

    2014-05-01

    Full Text Available Melucha grandicula sp. n. and M. perampla sp. n., (Hemiptera: Heteroptera: Coreidae: Coreinae: Nematopodini are described from Bolivia, Colombia, Paraguay and Peru. Melucha quadrivittis Stål, 1862, stat. restit., is resurrected from synonymy under M. phyllocnemis (Burmeister, 1835. Melucha ruficornis Breddin, 1903, syn. n., is synonymized under M. lineatella (Fabricius, 1803. An identification key for the known species of Melucha Amyot & Serville, 1843 is given. New distributional records for some previously known species are added.

  7. First report and morphological redescription of Teleonemia morio (Stål (Hemiptera, Tingidae in Annona squamosa L. (Annonaceae in Brazil

    Directory of Open Access Journals (Sweden)

    Sônia M. F. Broglio

    2012-03-01

    Full Text Available First report and morphological redescription of Teleonemia morio (Stål (Hemiptera, Tingidae in Annona squamosa L. (Annonaceae in Brazil. This is the first report of a severe attack of Teleonemia morio (Stål, 1855 (Hemiptera, Heteroptera, Tingidae on Annona squamosa L. (custard apple, causing up to 80% of losses of infested trees. In order to facilitate the identification of this insect pest, the adult female of T. morio is redescribed based on specimens collected in Palmeira dos Índios, Alagoas, Brazil.

  8. Identification of a new species of Aphis (Hemiptera: Aphididae) based on distinct morphology

    Science.gov (United States)

    Aphis elena Lagos-Kutz and Voegtlin, sp. nov. (Hemiptera: Aphididae), is described from specimens collected in Illinois, USA, on the North American native plant, Pycnanthemum virginianum (L.) T. Dur. & B.D. Jacks. ex B.L. Rob. & Fernald (Family: Lamiaceae). Both apterous and alate viviparae are desc...

  9. Descriptions of two new species of Sphenorhina (Hemiptera, Cercopidae, Tomaspidinae from the Neotropical region

    Directory of Open Access Journals (Sweden)

    Andressa Paladini

    2013-06-01

    Full Text Available Description of two new species of Sphenorhina (Hemiptera, Cercopidae, Tomaspidinae from the Neotropical region. Two new species of Sphenorhina Amyot & Serville, S. pseudoboliviana SP. NOV: from Bolivia and S. plata SP. NOV: from Argentina are described and illustrated.

  10. Preparing soft-bodied arthropods for arthropods for microscope examination: Armored Scales (Insects: Hemiptera: Diaspididae)

    Science.gov (United States)

    Proper identification of armored scales (Hemiptera: Diaspididae) requires preparation of the specimen on a microscope slide. This training video provides visual instruction on how to prepare armored scales specimens on microscope slides for examination and identification. Steps ranging from collect...

  11. Mitochondrial phylogenomics of Hemiptera reveals adaptive innovations driving the diversification of true bugs

    Science.gov (United States)

    Li, Hu; Leavengood, John M.; Chapman, Eric G.; Burkhardt, Daniel; Song, Fan; Jiang, Pei; Liu, Jinpeng; Cai, Wanzhi

    2017-01-01

    Hemiptera, the largest non-holometabolous order of insects, represents approximately 7% of metazoan diversity. With extraordinary life histories and highly specialized morphological adaptations, hemipterans have exploited diverse habitats and food sources through approximately 300 Myr of evolution. To elucidate the phylogeny and evolutionary history of Hemiptera, we carried out the most comprehensive mitogenomics analysis on the richest taxon sampling to date covering all the suborders and infraorders, including 34 newly sequenced and 94 published mitogenomes. With optimized branch length and sequence heterogeneity, Bayesian analyses using a site-heterogeneous mixture model resolved the higher-level hemipteran phylogeny as (Sternorrhyncha, (Auchenorrhyncha, (Coleorrhyncha, Heteroptera))). Ancestral character state reconstruction and divergence time estimation suggest that the success of true bugs (Heteroptera) is probably due to angiosperm coevolution, but key adaptive innovations (e.g. prognathous mouthpart, predatory behaviour, and haemelytron) facilitated multiple independent shifts among diverse feeding habits and multiple independent colonizations of aquatic habitats. PMID:28878063

  12. Hemiptera community and species responses to grassland sward islets

    OpenAIRE

    Helden, Alvin J.; Dittrich, Alex D. K.

    2016-01-01

    Sward islet is a term that has been used to describe a patch of longer vegetation in a pasture produced by a reduction in cattle grazing around their dung. They are known to affect the abundance and distribution of grassland arthropods. Hemiptera, like other groups, are found in higher densities within islets than the surrounding sward. Does this modify the community composition or is there just a density effect? Evidence from a paired (islets, non-islets) study at an Irish cattle-grazed site...

  13. Morphometrics of the Southern Green Stink Bug [Nezara viridula (L.) (Hemiptera: Pentatomidae)] Stylet Bundle

    Science.gov (United States)

    The southern green stink bug, Nezara viridula (L.) (Hemiptera: Pentatomidae), is a cosmopolitan pest of high-value cash crops, including cotton (Gossypium hirsutum L.; Malvales: Malvaceae). The pest can ingest and transmit disease-causing bacterial and fungal pathogens of cotton. We hypothesized t...

  14. Fooling the harlequin bug (Hemiptera: Pentatomidae) using synthetic volatiles to alter host plant choice

    Science.gov (United States)

    Harlequin bug, Murgantia histrionica (Hahn) (Hemiptera: Pentatomidae), is a widespread invasive pest that feeds on a variety of brassicaceous crops and other plants. To understand olfactory cues which mediate host-finding, and possible utility in pest management, we deployed aggregation pheromone (m...

  15. Attraction of stink bug (Hemiptera: Pentatomidae) nymphs to Euschistus aggregation pheromone in the field

    Science.gov (United States)

    Phytophagous stink bugs (Hemiptera: Pentatomidae) are primary pests in most fruit, vegetable, grain, and row crops worldwide. Pheromones have been identified and synthesized for several species of economically important stink bug pests. When yellow pyramid traps are baited with lures containing thes...

  16. Phylogenetic divergences of the true bugs (Insecta: Hemiptera: Heteroptera), with emphasis on the aquatic lineages

    DEFF Research Database (Denmark)

    Wang, Yan-hui; Cui, Ying; Rédei, Dávid

    2016-01-01

    Heteroptera are among the most diverse hemimetabolous insects. Seven infraorders have been recognized within this suborder of Hemiptera. Apart from the well-established sister-group relationship between Cimicomorpha and Pentatomomorpha (= Terheteroptera), the two terminal lineages, the relationsh...

  17. Association of Verde plant bug, Creontiades signatus (Hemiptera: Miridae), with cotton boll rot

    Science.gov (United States)

    Cotton along the Gulf Coast of south Texas has experienced loss from cotton boll rot especially during the last 10 to 15 years, and stink bugs and plant bugs (Hemiptera: Pentatomidae and Miridae) that feed on cotton bolls have been suspected in introducing the disease. A replicated grower field surv...

  18. Etude de quelques paramètres biologiques de Pseudotheraptus ...

    African Journals Online (AJOL)

    ciclo biológico de Crinocerus sanctus. (Fabricius, 1775) (Hemiptera: Coreidae) sob condições de laboratório. An. Soc. Entomol. Brasil., 15(1): 5-18. Amaral Filho B. F. & Cajueiro I. V. M., 1977. Observações sobre o ciclobiológico de. Veneza stigma (Herbest, 1784) Osuna, 1975. (Hemiptera, Coreidae) em laboratório. An.

  19. Stylet bundle morphology and trophically related enzymes of the hemlock woolly adelgid (Hemiptera: Adelgidae)

    Science.gov (United States)

    Kelly L.F. Oten; Allen C. Cohen; Fred P. Hain

    2014-01-01

    The hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae), is a pest of eastern and Carolina hemlocks (Tsuga canadensis (L.) Carriere and Tsuga caroliniana Engelmann, respectively) in the eastern United States and has already caused catastrophic changes to eastern forests. As one of the significant...

  20. An overview on the ecology of Triatominae (Hemiptera:Reduviidae).

    Science.gov (United States)

    Galvão, Cleber; Justi, Silvia A

    2015-11-01

    Chagas disease, the American trypanosomiasis, is an important neglected tropical illness caused by the flagellate protozoan Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) and transmitted by insects of the subfamily Triatominae (Hemiptera: Reduviidae). Here we provide an overview on the current knowledge about Triatominae ecology, its association with human, T. cruzi infection and the immediate consequences of habitat fragmentation. We also discuss the geographic distribution of the species and the importance of predicting their distributions to control programs. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. Relative Abundance of Carsonella ruddii (Gamma Proteobacterium) in Females and Males of Cacopsylla pyricola (Hemiptera: Psyllidae) and Bactericera cockerelli (Hemiptera: Triozidae)

    Science.gov (United States)

    Cooper, W. Rodney; Garczynski, Stephen F.; Horton, David R.

    2015-01-01

    Carsonella ruddii (Gamma Proteobacterium) is an obligate bacterial endosymbiont of psyllids that produces essential amino acids that are lacking in the insect’s diet. Accurate estimations of Carsonella populations are important to studies of Carsonella-psyllid interactions and to developing ways to target Carsonella for control of psyllid pests including pear psylla, Cacopsylla pyricola (Förster) (Hemiptera: Psyllidae) and potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae). We used two methods, namely fluorescence in situ hybridization and quantitative polymerase chain reaction (qPCR), to estimate relative abundance of Carsonella in bacteriocytes and whole bodies of psyllids, respectively. Using these two methods, we compared Carsonella populations between female and male insects. Estimations using fluorescence in situ hybridization indicated that Carsonella was more abundant in bacteriocytes of female C. pyricola than in those of males, but Carsonella abundance in bacteriocytes did not differ between sexes of B. cockerelli. Analyses by qPCR using whole-body specimens indicated Carsonella was more abundant in females than in males of both psyllids. Neither fluorescence in situ hybridization nor qPCR indicated that Carsonella populations differed in abundance among adults of different ages (0–3 wk after adult eclosion). Using fluorescence in situ hybridization, Carsonella was observed in ovarioles of newly emerged females and formed an aggregation in the posterior end of mature oocytes. Results of our study indicate that female psyllids harbor greater populations of Carsonella than do males and that sex should be controlled for in studies which require estimations of Carsonella populations. PMID:26056318

  2. Allozyme Variation in Hemlock Woolly Adelgid (Hemiptera: Adelgidae) from the United States and China

    Science.gov (United States)

    V. S& #225; nchez; M.A. Keena; M.A. Keena

    2009-01-01

    The hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae), is a major introduced pest of eastern hemlock, Tsuga canadensis (L.) Carriere. Hemlock woolly adelgid in the United States is anholocyclic and an obligate parthenogen, because no suitable primary host (on which sexual reproduction occurs in Asia) is...

  3. First record of Acizzia jamatonica (Hemiptera: Psyllidae) in North America: friend or foe?

    Science.gov (United States)

    Michael D. Ulyshen; Douglass Miller

    2007-01-01

    Acizzia jamatonica (Kuwayama) (Hemiptera: Sternorrhyncha: Psyllidae) is reported for the first time in North America. Because the species is thought to feed exclusively on Albizia, it may prove to be an effective biocontrol agent against the invasive Albizia julibrissin Durazzini in the southeastern United States. Because A. julibrissin is also an ornamental plant of...

  4. Effects of founder population size on the performance of Orius laevigatus (Hemiptera: Anthocoridae) colonies

    NARCIS (Netherlands)

    Castañe, C.; Bueno, V.H.P.; Carvalho, L.M.; Lenteren, van J.C.

    2014-01-01

    Orius laevigatus (Hemiptera: Anthocoridae) is a key predator of thrips and is mass reared in large numbers for use in biological control. The aim of this study was to evaluate the effect of founder population size on the biological and behavioral performance of O. laevigatus over time. Laboratory

  5. Spatial Distribution and Coexisting Patterns of Adults and Nymphs of Tibraca limbativentris (Hemiptera: Pentatomidae) in Paddy Rice Fields.

    Science.gov (United States)

    Alves, Tavvs M; Maia, Aline H N; Barrigossi, José A F

    2016-12-01

    The rice stem stink bug, Tibraca limbativentris Stål (Hemiptera: Pentatomidae), is a primary insect pest of paddy rice in South America. Knowledge of its spatial distribution can support sampling plans needed for timely decisions about pest control. This study aimed to investigate the spatial distribution of adults and nymphs of T. limbativentris and determine the spatial coexistence of these stages of development. Fifteen paddy rice fields were scouted once each season to estimate insect densities. Scouting was performed on regular grids with sampling points separated by ∼50 m. Moran's I and semivariograms were used to determine spatial distribution patterns. Spatial coexistence of nymphs and adults was explored via spatial point process. Here, adults and nymphs had typically contrasting spatial distribution patterns within the same field; however, the frequency of aggregation was not different between these developmental stages. Adults and nymphs were aggregated in seven fields and randomly distributed in the other eight fields. Uniform distribution of adults or nymphs was not observed. The study-wide semivariogram ranges were ∼40 m for adults and ∼55 m for nymphs. Nymphs and adults spatially coexisted on 67% of the fields. Coexisting patterns were classified using one of the following processes: stage-independent, bidirectional attractive, unidirectional attractive, bidirectional inhibiting, or unidirectional inhibiting. The information presented herein can be important for developing sampling plans for decision-making, implementing tactics for site-specific management, and monitoring areas free of T. limbativentrisResumoO percevejo-do-colmo Tibraca limbativentris Stål (Hemiptera: Pentatomidae) é uma praga primária na cultura do arroz irrigado na América do Sul. O conhecimento de sua distribuição espacial é essencial para desenvolver planos de amostragem e para o controle desta praga. Nosso objetivo foi investigar a distribuição espacial de

  6. The genus Alphocoris in the Indomalaya (Hemiptera: Heteroptera: Scutelleridae).

    Science.gov (United States)

    RÉdei, DÁvid; Tsai, Jing-Fu; Jindra, ZdenĚk

    2018-02-21

    The Indomalayan species of the genus Alphocoris Germar, 1839 (Hemiptera: Heteroptera: Scutelleridae: Odontotarsinae: Odontotarsini) are revised. Three species, A. caudatus Rédei, Tsai Jindra, sp. nov. (India: Maharashtra), A. naso Rédei Tsai, sp. nov. (India: Goa), and A. asper Rédei, Tsai Jindra, sp. nov. (India: Goa and Maharashtra), are recognized. The type material of A. lixoides Germar, 1839 (type locality: Senegal) is documented; previous records of this species from India and Pakistan are considered as based on misidentifications, the species is restricted to the Afrotropical Region.

  7. Diaspididae (Hemiptera, Coccoidea in sori of two fern species

    Directory of Open Access Journals (Sweden)

    Marcelo Santos

    2015-12-01

    Duas Espécies de Cochonilhas (Hemiptera: Diaspididae Associadas com Soros de Samambaias Resumo. A presente comunicação relata a presença de duas espécies de cochonilhas Hemiberlesia palmae (Cockerell e Pinnaspis strachani (Cooley (Coccoidea, Diaspididae, associadas respectivamente com Asplenium serratum L. (Aspleniaceae e Niphidium crassifolium (L. Lellinger (Polypodiaceae. É o primeiro registro de uma samambaia como planta hospedeira de Hemiberlesia palmae.  Nas duas espécies de samambaias, os diaspidídeos encontravam-se concentrados principalmente ao redor dos soros.

  8. Monitoramento do percevejo marrom Euschistus heros (Hemiptera: Pentatomidae por feromônio sexual em lavoura de soja

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    Valvenarg Pereira da Silva

    2014-11-01

    Full Text Available O objetivo deste trabalho foi avaliar a eficiência das formulações septo de borracha e lure do feromônio sexual do percevejo marrom Euschistus heros e compará-la ao método do pano de batida, para o monitoramento dessa praga em lavoura de soja. Os tratamentos foram: feromônio em septo de borracha, feromônio em lure e amostragem com pano de batida. As amostragens foram realizadas semanalmente durante a fase reprodutiva da soja (dez semanas. Para estimar a densidade populacional de percevejos e compará-la à captura nas armadilhas de feromônio, foram efetuados quatro panos de batida por semana em todas as parcelas; além disso, foi determinado o número de percevejos capturados nas armadilhas por semana. Os danos provocados pelos percevejos nas sementes foram analisados pelo teste de tetrazólio. As formulações do feromônio foram eficientes na captura de E. heros, e a formulação em lure capturou mais percevejos. O controle precoce da população, indicado pelo nível de controle nas armadilhas com feromônio, resultou em sementes com maior qualidade do que as provenientes das parcelas monitoradas pelo pano de batida. Assim, as armadilhas com feromônio são mais eficientes que o pano de batida para o monitoramento das populações de percevejos durante a fase crítica do ataque deste inseto nas lavouras de soja.

  9. Mating disruption of Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae) by playback of vibrational signals in vineyard trellis

    Science.gov (United States)

    BACKGROUND: Glassy-winged sharpshooter (GWSS), Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), is an important vector of the bacterium Xylella fastidiosa, the causal agent of Pierce’s disease of grapevine. Area-wide applications of neonicotinoid insecticides have suppressed GWSS populati...

  10. The cicada genus Karenia Distant, 1888 (Hemiptera: Cicadidae), with description of a new species.

    Science.gov (United States)

    Pham, Hong-Thai; Constant, Jerome

    2014-08-19

    The cicadas of the genus Karenia are reviewed, and Karenia tibetensis sp.nov. (Hemiptera: Cicadidae) is described from Tibet, China. Pictures of the male adult and illustrations of the male genitalia are provided. A key to the species of Karenia is presented and the distribution of the Karenia species is discussed.

  11. Field evaluation of potential pheromone lures for Lygus lineolaris (Hemiptera: Miridae) in the Mid-South

    Science.gov (United States)

    Plant bugs (Hemiptera: Miridae) are phytophagous pests of cultivated plants around the world. In the mid-South region of the United States, Lygus lineolaris is a primary pest of cotton, and causes economic damage. Previously published research about the volatiles produced by members of the genus Lyg...

  12. Biologia floral de Croton urucurana Baill. (Euphorbiaceae ocorrente em vegetação ripária da ilha Porto Rico, Porto Rico, Estado do Paraná, Brasil - DOI: 10.4025/actascibiolsci.v26i2.1638 Floral biology of Croton urucurana Baill (Euphorbiaceae that occurs in riparian vegetation of Porto Rico Island, in Porto Rico, Paraná, Brazil - DOI: 10.4025/actascibiolsci.v26i2.1638

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    Luiz Antonio de Souza

    2004-04-01

    Full Text Available Foram realizados estudos de biologia floral em Croton urucurana, espécie monóica, arbórea, pioneira e recomendada para a recuperação de matas ciliares. As flores são diclinas, pequenas, apresentam coloração amarelo-esverdeada e dispõem-se em inflorescências racemosas. A antese é noturna, por volta das 23h, e as flores duram três dias. Os visitantes florais foram insetos das ordens Diptera, Odonata, Lepidoptera, Hemiptera e Hymenoptera (Apidae e Vespidae, sendo Apis mellifera a espécie mais freqüenteStudies of floral biology were developed on Croton urucurana, a pioneer monoecious tree, recommended to be used in the process of regeneration of the riparian forest. The plant presents small yellow-greenish unisexual flowers, disposed in racemose inflorescences. Anthesis occurs at night, flowers last three days and open around 11 pm. Flowers are visited by insects belonging to the following Orders Diptera, Odonata, Lepidoptera, Hemiptera and Hymenoptera (Apidae and Vespidae. Apis mellifera was the most frequent flower visitor

  13. Dinâmica populacional do parasitoide de ovos erythmelus tingitiphagus (hymenoptera: mymaridae em clone de seringueira, em Itiquira, MT Population dynamic of egg parasitoid erythmelus tingitiphagus (hymenoptera: mymaridae in rubber tree clone in Itiquira, Mato Grosso State, Brazil

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    Rodrigo Souza Santos

    2013-04-01

    Full Text Available O percevejo-de-renda Leptopharsa heveae Drake & Poor (Hemiptera: Tingidae é uma das mais importantes pragas da heveicultura no Brasil, principalmente nas regiões Sudeste e Centro-Oeste. Devido ao seu hábito sugador, na face abaxial das folhas, esta praga leva à senescência precoce das mesmas e a reduções na produção de látex em até 30%. Dentre os inimigos naturais de L. heveae está o parasitoide de ovos Erythmelus tingitiphagus (Soares (Hymenoptera: Mymaridae, regulando suas populações em condições naturais. O objetivo deste estudo foi verificar a dinâmica populacional deste parasitoide, bem como correlacioná-la com os fatores meteorológicos temperatura e pluviosidade, em plantio comercial de seringueira do clone PB 217, em Itiquira, MT. Semanalmente foram coletadas quatro folhas maduras por árvore, no terço inferior da copa de 40 árvores, totalizando 160 folhas por amostragem, no período de agosto de 2006 a janeiro de 2007. Houve correlação positiva entre a dinâmica populacional e os fatores meteorológicos, sendo o pico populacional do parasitoide observado no mês de novembro e declinando até janeiro na área estudada.The lace bug Leptopharsa heveae Drake & Poor (Hemiptera: Tingidae is one of the most import heveiculture pests in Brazil, mainly in the southeast and central-west regions. Due to its sucking habit on the abaxial surface, this pest causes precocious senescence in leaves, and leads to the reduction in latex production in up to 30% of them. Among the natural enemies of L. heveae is the egg parasitoid Erythmelus tingitiphagus (Soares (Hymenoptera: Mymaridae, regulating populations of L. heveae in natural conditions. The objective of this work was to verify the population dynamics of this parasitoid, as well as to correlate it with meteorological factors such as temperature and rainfall, in a commercial plantation of rubber trees of the PB 217 clone, in Itiquira, in the Mato Grosso state, Brazil. Four

  14. Duração do período ninfal e sobrevivência do predador Podisus connexivus Bergroth (Hemiptera, Pentatomidae, em três presas alternativas Ninfal period duration and survival of the predator Podisus connexivus Bergroth (Hemiptera, Pentatomidae, in three alternative preys

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    José Cola Zanuncio

    1993-01-01

    Full Text Available Longevity and survival of the predator Podisus connexivus (Hemiptera: Pentatomidae were studied in three alternative preys: T1 - Bombyx mori (Lepidoptera, Bombycidae catterpilars; T2 - Musca domestica (Diptera, Muscidae larva and T3 - Tenebrio molitor (Coleoptera, Tenebrionidae larva. Longevity and survival were: 22,1±0,6 days and 54,3±5,3%; 25,2±1,3 days and 56,0±4,9% and 22,0±0,8 days and 34,6±8,6%, for treatments T1, T2 and T3, respectively. Comparing to other researches, a lower survival was found. This is probably because a F2 generation from field material, was used. Since the insect was not well adapted to the laboratory conditions this could have led to lower survival.

  15. Infestação de Aetalion reticulatum (Linnaeus (Hemiptera: Auchenorrhyncha: Aethalionidae em Plantas de Euterpe oleracea Martius (Arecaceae no Estado do Acre

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    Rodrigo Santos

    2015-04-01

    Abstract. The açai palm (Euterpe oleracea Martius is a native palm tree from the Amazon region floodplains and may be indicated as the most economically profitable species of this genus. Its fruit pulp is extracted and widely consumed by the population of northern Brazil in several ways. With the expansion of the planted area many factors may affect the production and limit the cultivation, highlighting the occurrence of insect pests that cause losses in production. This report presents the first occurrence of the leafhopper Aetalion reticulatum (Linnaeus (Hemiptera: Auchenorrhyncha: Aethalionidae in E. oleracea plants. The occurrence of this insect associated with açai palms in Rio Branco, Acre, increases the list of hosts for this leafhopper in Brazil and reinforces the need for studies of population dynamics, survey of natural enemies, levels of damage and control methods in order to anticipate population outbreaks of this pest in commercial plantations of açai palms in the State.

  16. Atividade de buprofezin sobre a cigarrinha verde do feijoeiro Empoasca kraemeri (Ross & Moore, 1957 (Hemiptera, Cicadellidae em condições de laboratório Activity of buprofezin on the green leafhopper Empoasca kraemeri (Ross & Moore, 1957 (Hemiptera, Cicadellidae under laboratory conditions

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    Paulo Rogério Moreno

    2002-09-01

    Full Text Available A espécie Empoasca kraemeri é uma importante praga para a cultura do feijoeiro (Phaseolus vulgaris L. em toda a América Latina, sendo necessário recorrer ao controle químico quando a praga atinge alta população. Neste trabalho, foi estudado o efeito do inseticida buprofezin sobre ninfas de primeiro instar de Empoasca kraemeri (Hemiptera, Cicadellidae, sob condições de laboratório. Esta fase da praga mostrou-se altamente susceptível ao inseticida, sendo a CL50 estimada de 0,112 mg L-1. Em outro bioensaio, casais virgens e recém-emergidos foram colocados em contato com plantas de feijoeiro previamente imersas na calda do inseticida. A longevidade média para fêmeas/machos foi de: 46/27, 18/16 e 16/14 dias para a testemunha, 10 e 50 mg L-1 de buprofezin, respectivamente. Observou-se uma média de 102; 0,9 e 0,1 ovos por fêmea no tratamento testemunha, 10 e 50 mg L-1 de buprofezin, respectivamente. O ritmo de postura, que determina a longevidade dos indivíduos, foi de 2,31; 0,09 e 0,01 ovos/fêmea/dia para a testemunha, 10 e 50 mg L-1 de buprofezin, respectivamente. No último bioensaio, foi investigado o efeito de buprofezin sobre os adultos, porém, tratando-se apenas fêmeas e machos isoladamente com o inseticida em superfície contaminada a 1000 mg L-1 de buprofezin e posteriormente, colocando-os junto a insetos do sexo oposto que não haviam sido contaminados. Foi observada longevidade para fêmeas/machos de: 46/27, 35/23 e 27/13 dias para o tratamento testemunha, fêmeas tratadas e machos tratados, respectivamente. O ritmo de postura não apresentou diferença, sendo de 2,31; 2,56 e 2,42 ovos/fêmea/dia no tratamento testemunha, fêmeas tratadas e machos tratados, respectivamente.Empoasca kraemeri is a key pest of dry beans (Phaseolus vulgaris L. in Latin America and chemical control is necessary when the pest reaches high infestation levels (threshold. The objective of this work was to study the effect of buprofezin on first

  17. Two pests overlap: Drosophila suzukii (Diptera: Drosophilidae) use of fruit exposed to Halyomorpha halys (Hemiptera: Pentatomidae)

    Science.gov (United States)

    Spotted wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), and brown marmorated stink bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) are global economic pests. Both pests may co-occur on small fruits, and we investigated whether fruit recently exposed to H. halys woul...

  18. Molecular and morphological identification of the mealybug pest species, Phenacoccus solani Ferris (Hemiptera: Pseudococcidae), in Egypt

    Science.gov (United States)

    During the summer and autumn of 2016, heavy infestations of the mealybug, Phenacoccus solani Ferris (Hemiptera: Pseudococcidae), were observed on pumpkins, Cucurbita spp. (Cucurbitaceae). This was the first record of the species in Egypt. Several populations have been collected in various pumpkin fr...

  19. Effects of atmospheric pressure trends on calling, mate-seeking, and phototaxis of Diaphorina citri (Hemiptera: Liviidae)

    Science.gov (United States)

    Insects and other animals sometimes modify behavior in response to changes in atmospheric pressure, an environmental cue that can provide warning of potentially injurious windy and rainy weather. To determine if Diaphorina citri (Hemiptera: Liviidae) calling, mate-seeking, and phototaxis behaviors w...

  20. Two new planthopper species (Hemiptera, Fulgoroidea, Caliscelidae) collected in pitfall traps in Zambia.

    Science.gov (United States)

    Chmurova, Lucia; Webb, Michael D

    2016-08-22

    Two new species of planthoppers in the family Caliscelidae (Hemiptera: Fulgoroidea) are described from Zambia, i.e., Afronaso spinosa sp. n. and Calampocus zambiaensis sp. n. All specimens are flightless males and nearly all were collected from baited pitfall traps (except for one specimen collected from a yellow pan trap), suggesting that they live near to or on the ground.

  1. Complete DNA sequence of the mitochondrial genome of the treehopper Leptobelus gazella (Membracoidea: Hemiptera).

    Science.gov (United States)

    Zhao, Xing; Liang, Ai-Ping

    2016-09-01

    The first complete DNA sequence of the mitochondrial genome (mitogenome) of Leptobelus gazelle (Membracoidea: Hemiptera) is determined in this study. The circular molecule is 16,007 bp in its full length, which encodes a set of 37 genes, including 13 proteins, 2 ribosomal RNAs, 22 transfer RNAs, and contains an A + T-rich region (CR). The gene numbers, content, and organization of L. gazelle are similar to other typical metazoan mitogenomes. Twelve of the 13 PCGs are initiated with ATR methionine or ATT isoleucine codons, except the atp8 gene that uses the ATC isoleucine as start signal. Ten of the 13 PCGs have complete termination codons, either TAA (nine genes) or TAG (cytb). The remaining 3 PCGs (cox1, cox2 and nad5) have incomplete termination codons T (AA). All of the 22 tRNAs can be folded in the form of a typical clover-leaf structure. The complete mitogenome sequence data of L. gazelle is useful for the phylogenetic and biogeographic studies of the Membracoidea and Hemiptera.

  2. Time course study of feeding damage to pin head cotton squares by Lygus hesperus (Hemiptera: Miridae)

    Science.gov (United States)

    Lygus hesperus (Hemiptera: Miridae) is an economically important pest affecting cotton crops in California. Lygus feeding causes abscission of cotton squares, with damage severity dependent on size of the square and life stage of the insect. Fifth instar nymphs are the most damaging stage; however, ...

  3. Side effects of some pesticides on the silverleaf whitefly’s parasitoid, Eretmocerus mundus Mercet (Hymenoptera: Aphelinidae under laboratory conditions

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    Mustafa PORTAKALDALI

    2015-12-01

    Full Text Available The silverleaf whitefly, Bemisia tabaci (Gennadius (Hemiptera: Aleyrodidae is one of major pests in greenhouses vegetable cultivation. Eretmocerus mundus Mercet (Hymenoptera: Aphelinidae has an important role among effective parasitoids used in biological control of the silverleaf whitefly. Nondestructive or less destructive pesticides against this parasitoid should be preferred in the scope of biological control. For this goal, side effects of 8 plant protection products, including spinosad, pyriproxyfen, abamectin, emamectin benzoate, copper oxychloride, kresoxim-methyl+boscalid, dimethoate and propineb+cymoxanil on pupae and adults of E. mundus were tested in this laboratory study conducted according to IOBC standards. As a result, pesticides containing spinosad, abamectin and dimethoate were determined as highly destructive to pupae and adults of E. mundus, and pesticide containing emamectin benzoate was found as moderately harmful. As for propineb+cymoxanil, pyriproxyfen, kresoxim-methyl+boscalid and copper oxychloride, they were determined as ineffective on the parasitoid under laboratory conditions. These results obtained from experiments that were conducted under laboratory conditions also have to be supported by field or semi-field experiment’s results.

  4. The Salicylic Acid-Mediated Release of Plant Volatiles Affects the Host Choice of Bemisia tabaci

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    Xiaobin Shi

    2016-06-01

    Full Text Available The whitefly Bemisia tabaci (Gennadius (Hemiptera: Aleyrodidae causes serious crop losses worldwide by transmitting viruses. We have previously shown that salicylic acid (SA-related plant defenses directly affect whiteflies. In this study, we applied exogenous SA to tomato plants in order to investigate the interaction between SA-induced plant volatiles and nonviruliferous B. tabaci B and Q or B- and Q-carrying tomato yellow leaf curl virus (TYLCV. The results showed that exogenous SA caused plants to repel nonviruliferous whiteflies, but the effect was reduced when the SA concentration was low and when the whiteflies were viruliferous. Exogenous SA increased the number and quantity of plant volatiles—especially the quantity of methyl salicylate and δ-limonene. In Y-tube olfactometer assays, methyl salicylate and δ-limonene repelled the whiteflies, but the repellency was reduced for viruliferous Q. We suggest that the release of plant volatiles as mediated by SA affects the interaction between whiteflies, plants, and viruses. Further studies are needed to determine why viruliferous Q is less sensitive than nonviruliferous Q to repellent plant volatiles.

  5. Biology of Leptoypha hospita (Hemiptera: Tingidae), a Potential Biological Control Agent of Chinese Privet

    Science.gov (United States)

    Yanzhuo Zhang; James L. Hanula; Scott Horn; Kristine Braman; Jianghua Sun

    2011-01-01

    The biology of Leptoypha hospita Drake et Poor (Hemiptera: Tingidae), a potential biological control agent from China for Chinese privet, Ligustrum sinense Lour., was studied in quarantine in the United States. Both nymphs and adults feed on Chinese privet mesophyll cells that lead to a bleached appearance of leaves and dieback of branch tips. L. hospita has five...

  6. Contributions to the knowledge of Banasa Stål (Hemiptera, Heteroptera, Pentatomidae: Banasa chaca Thomas

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    Thereza de Almeida Garbelotto

    2011-09-01

    Full Text Available Contributions to the knowledge of Banasa Stål (Hemiptera, Heteroptera, Pentatomidae: Banasa chaca Thomas. The male of Banasa chaca Thomas is described with emphasis on external and internal genitalia and the female internal genitalia is described. Banasa chaca is newly recorded from Buenos Aires Province (Argentina.

  7. Eficiência de inseticidas em tratamento de sementes de milho no controle da cigarrinha Dalbulus maidis (Hemiptera: Cicadellidae em viveiro telado Insecticides efficiency in treatment of corn seeds to control leafhopper Dalbulus maidis (Hemiptera: Cicadellidae in greenhouse

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    Charles Martins de Oliveira

    2008-02-01

    Full Text Available O objetivo deste trabalho foi verificar a eficiência de inseticidas no tratamento de sementes no controle da cigarrinha-do-milho em viveiro telado. Sementes de milho foram tratadas ou não com inseticidas (imidacloprid, thiamethoxan, thiodicarb + zn, thiodicarb, carbofuran, carbofuran + zn e carbosulfan e semeadas em vasos plásticos. Nas plantas, foram confinadas cigarrinhas sadias e avaliada a eficiência desses inseticidas no controle desse inseto, em diferentes intervalos de tempo e em períodos sucessivos. Os produtos imidacloprid e thiamethoxan foram os mais eficientes no controle da cigarrinha, proporcionando eficiência de controle de adultos de D. maidis igual ou superior a 70%, até o trigésimo dia de avaliação, após 4 a 24h de confinamento das cigarrinhas.This research was aimed at checking the efficiency of insecticide seed treatment on corn leafhopper control, at greenhouse. Maize seeds were treated with insecticides (imidacloprid, thiamethoxan, thiodicarb + zn, thiodicarb, carbofuran, carbofuran + zn and carbosulfan and sowed on plastic pots. On those plants healthy leafhoppers were confined, and the efficiency of those insecticides on its control was evaluated, at different intervals of time and in successive periods. The insecticides imidacloprid and thiamethoxan were the most efficient to control the corn leafhoppers, and provided control efficiency of D. maidis adults equal or upper 70% until the thirtieth day of evaluation, after 4 to 24h of leafhoppers confining.

  8. A NEW SPECIES OF BEAMERANA YOUNG, 1952 (HEMIPTERA: CICADELLIDAE: TYPHLOCYBINAE FROM SOUTHEASTERN BRAZIL

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    Luci Boa Nova Coelho

    2014-05-01

    Full Text Available A new species of the Neotropical leafhopper genus Beamerana Young, 1952 (Hemiptera: Cicadellidae: Typhlocybinae is described and illustrated based on the specimens from Minas Gerais, southeastern Brazil. Beamerana boomerang sp. nov. differs from its congeners by the fore wing venation and the male genitalia. A key to the species of Beamerana is provided. The genus is recorded for Brazil for the first time.

  9. Effect of Two Entomopathogenic Fungi in Controlling Aleurodicus cocois (Curtis, 1846 (Hemiptera: Aleyrodidae Efecto de Dos Hongos Entomopatógenos en el Control de Aleurodicus cocois (Curtis, 1846 (Hemiptera: Aleyrodidae

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    Elizabeth Núñez del Prado

    2008-03-01

    Full Text Available Aleurodicus cocois (Curtis, 1846, the coconut whitefly, is a very damaging pest in Peru, mainly in avocado trees (Persea americana Mill.. It has been determined that entomopathogenic fungi can infect and kill white flies and can be used as biological control agents. The object of this research was to determine if there is any synergic action of the entomopathogenic fungi Paecilomyces fumosoroseus (Wize Brown & Smith 1957 and Verticillium lecanii (Zimmerman, 1892 Viégas 1939, in controlling A. cocois. Plastic sterile dishes were prepared where 5 mL 2% agar were used for each stage; leaves infested with A. cocois were placed upon these. Three different treatments were applied by sprinkling: P. fumosoroseus (P, V. lecanii (V or the mixture of the two fungi. These treatments were evaluated at one, two and seven days in the case of nymph I and eggs, and after four, eight and 12 days for nymph II, NIII and NIV. The lethal effect of V. lecanii on the second nymph instar of A. cocois was better than that of P. fumosoroseus and than the mixture of both fungi; the highest mortality percentage appeared from day eight to day 12. A small increase was observed in the effect of the mixture of the two entomopathogenic fungi on the IV nymph instar; and the effect of P. fumosoroseus was the lowestAleurodicus cocois (Curtis, 1846, la mosca blanca del cocotero, constituye una plaga muy importante en el Perú, principalmente en el palto (Persea americana Mill.. Se ha determinado que los hongos entomopatógenos pueden infectar y matar a la mosca blanca, por lo que podrían ser usados como agentes de control biológico. El objetivo del presente trabajo fue determinar si existe alguna acción conjunta de dos hongos entomopatógenos Paecilomyces fumosoroseus (Wize Brown & Smith 1957 y Verticillium lecanii (Zimmerman, 1892 Viégas 1939, en el control de A. cocois. Para ello se prepararon previamente placas de plástico estériles en las que se sirvieron aproximadamente 5 mL de agar al 2%, donde se colocaron hojas infestadas con A. cocois, aplicándose tres tratamientos por aspersión: P. fumosoroseus (P, V. lecanii (V y la mezcla de los dos hongos, los cuales se evaluaron en el caso de Ninfa I y huevos después de uno, dos y siete días, y para la Ninfas II, NIII y NIV cada cuatro, ocho y 12 días. El efecto de mortalidad de V. lecanii sobre el segundo estadío ninfal de A. cocois fue mejor que P. fumosoroseus y que la mezcla de ambos hongos, ya que el mayor porcentaje de mortalidad se presentó desde el octavo día hasta el día 12. En el caso del IV estadío ninfal se observó un ligero incremento en el efecto al combinarse los dos hongos entomopatógenos evaluados, siendo menor el efecto en P. fumosoroseus

  10. First record in Argentina of Drepanosiphum oregonense Granovsky (Hemiptera: Aphididae) found on maples (Sapindaceae)

    OpenAIRE

    Ortego, Jaime; Olave, Anabel; Mier Durante, Milagros Pilar

    2014-01-01

    Se informa del hallazgo del áfido Drepanosiphum oregonense Granovsky (Hemiptera: Aphididae) sobre árboles de Acer pseudoplatanus y A. campestre (Sapindaceae) en Argentina lo que constituye el primer registro de la especie y del género Drepanosiphum Koch en Argentina y el segundo en Sudamérica. The finding in Argentina of the aphid Drepanosiphum oregonense on "maple tree" Acer pseudoplatanus ...

  11. Predation of Apiomerus pilipes (Fabricius (Hemiptera, Reduviidae, Harpactorinae, Apiomerini over Meliponinae bees (Hymenoptera, Apidae, in the State of Amazonas, Brazil

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    Alexandre Coletto da Silva

    2004-12-01

    Full Text Available The present work shows the occurrence of an intense predatory activity on adults working Meliponinae bees (Hymenoptera, Apidae, by Apiomerus pilipes (Fabricius, 1787 (Hemiptera, Reduviidae, Harpactorinae, Apiomerini at a meliponary in the Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Amazonas State, Brazil.O presente trabalho registra a ocorrência de intensa atividade predatória de Apiomerus pilipes (Fabricius, 1787 (Hemiptera, Reduviidae, Harpactorini, Apiomerini sobre operárias adultas de meliponíneos (Hymenoptera, Apidae, no meliponário experimental do Instituto Nacional de Pesquisas da Amazônia (INPA, Manaus, Estado do Amazonas, Brasil. O meliponário se encontra num fragmento de vegetação secundária no próprio INPA.

  12. Evaluation of hemlock (Tsuga) species and hybrids for resistance to Adelges tsugae (Hemiptera: Adelgidae) using artificial infestation

    Science.gov (United States)

    Michael E. Montgomery; S.E. Bentz; Richard T. Olsen

    2009-01-01

    Hemlock (Tsuga) species and hybrids were evaluated for resistance to the hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae). The adelgid was accidentally introduced from Asia to the eastern United States, where it is causing widespread mortality of the native hemlocks, Tsuga canadensis (L.)...

  13. Pheromone of the banana-spotting bug, amblypelta lutescens lutescens Distant (Hemiptera: Coreidae): identification, synthesis and field bioassay

    Science.gov (United States)

    The banana spotting bug Amblypelta lutescens lutescens Distant (Hemiptera: Coreidae) is one of the principal pests of tree fruits and nuts across northern and eastern Australia. Apart from damage assessments in orchards, there are currently no other methods for monitoring bug activity to aid manage...

  14. Potential transmission of Pantoea spp. and Serratia marcescens (Enterobacteriales: Enterobacteriaceae) to plants by Lygus hesperus (Hemiptera: Miridae)

    Science.gov (United States)

    Lygus hesperus Knight (Hemiptera: Miridae) is a key agricultural pest in the western United States. In a recent study, proteins from Pantoea ananatis and Serratia marcescens (Enterobacteriales: Enterobacteriaceae) were identified in diet that was stylet-probed and fed upon by L. hesperus adults. P...

  15. Direct and indirect impacts of infestation of tomato plant by Myzus persicae (Hemiptera: Aphididae on Bemisia tabaci (Hemiptera: Aleyrodidae.

    Directory of Open Access Journals (Sweden)

    Xiao-Ling Tan

    Full Text Available The impacts of infestation by the green peach aphid (Myzus persicae on sweetpotato whitefly (Bemisia tabaci settling on tomato were determined in seven separate experiments with whole plants and with detached leaves through manipulation of four factors: durations of aphid infestation, density of aphids, intervals between aphid removal after different durations of infestation and the time of whitefly release, and leaf positions on the plants. The results demonstrated that B. tabaci preferred to settle on the plant leaves that had not been infested by aphids when they had a choice. The plant leaves on which aphids were still present (direct effect had fewer whiteflies than those previously infested by aphids (indirect effect. The whiteflies were able to settle on the plant which aphids had previously infested, and also could settle on leaves with aphids if no uninfested plants were available. Tests of direct factors revealed that duration of aphid infestation had a stronger effect on whitefly landing preference than aphid density; whitefly preference was the least when 20 aphids fed on the leaves for 72 h. Tests of indirect effects revealed that the major factor that affected whitefly preference for a host plant was the interval between the time of aphid removal after infestation and the time of whitefly release. The importance of the four factors that affected the induced plant defense against whiteflies can be arranged in the following order: time intervals between aphid removal and whitefly release > durations of aphid infestation > density of aphids > leaf positions on the plants. In conclusion, the density of aphid infestation and time for which they were feeding influenced the production of induced compounds by tomatoes, the whitefly responses to the plants, and reduced interspecific competition.

  16. Dinâmica populacional e evolução de dano de Triozoida limbata (Hemiptera: Psillydae em goiabeira, em Jaboticabal, SP Population dynamic and evolution of damage of Triozoida limbata (Hem.: Psillydae in guava orchard in Jaboticabal city, State of São Paulo

    Directory of Open Access Journals (Sweden)

    Carlos Alexandre Colombi

    2009-04-01

    Full Text Available Objetivou-se, no presente trabalho, conhecer a dinâmica populacional de Triozoida limbata em um pomar de goiaba submetido ao uso mínimo de inseticida no município de Jaboticabal - SP. Para o estudo da flutuação populacional e a evolução do dano do psilídeo foram analisadas quinzenalmente vinte plantas do pomar, no período de março de 2005 a junho de 2006. Para cada planta foram avaliadas dez folhas contendo o sintoma do ataque do psilídeo. Os parâmetros avaliados foram o número de ninfas e a porcentagem de dano causado nas folhas, de acordo com uma escala de notas. Esses parâmetros foram analisados em histogramas e correlacionados com os fatores meteorológicos. A densidade populacional do psilídeo aumentou com o surgimento das brotações e apresentou uma correlação positiva com a temperatura. O dano do psilídeo acompanhou a densidade populacional da praga. As maiores densidades populacionais de psilídeo ocorrem nos meses de setembro a novembro; e as menores entre os meses de maio a julho.The present work aimed at learning the population dynamic of Triozoida limbata (Enderlein, 1918 (Hemiptera: Psyllidae in a guava orchard submitted to the minimum use of insecticide in Jaboticabal - SP. For the study of population fluctuation and the evolution of damage from the psillid we analyzed 20 plants of the orchard biweekly, during the period from March 2005 to June 2006. For each plant we evaluated 10 leaves containing the symptom of the attack from the psillid. The evaluated parameters were the number of nymphs and the percentage of damage caused in the leaves, in accordance with a scale of notes. These parameters were analyzed in histograms and correlated to the meteorological factors. The population density of the psillid increased considerably with the approach of the spring and presented a positive correlation with the temperature. The level of damage of the psillid accompanied the population density of the pest. According to

  17. First record of Sesbania punicea (Fabales: Fabaceae) as a host plant for Chinavia hilaris (Hemiptera: Pentatomidae)

    Science.gov (United States)

    The green stink bug, Chinavia hilaris (Say) (Hemiptera: Pentatomidae), is an economic pest of cotton, Gossypium hirsutum L. Numerous known non-crop hosts of C. hilaris that exist in field edges bordering cotton are sources of this stink bug in this crop. Sesbania punicea plants in a field border su...

  18. Assesing Bemisia tabaci (Genn. biotype B resistance in soybean genotypes: Antixenosis and antibiosis Evaluación de la resistencia de genotipos de soya a Bemisia tabaci (Genn. biotipo B: Antixenosis y antibiosis

    Directory of Open Access Journals (Sweden)

    José Paulo Gonçalves Franco da Silva

    2012-12-01

    Full Text Available Since it was first reported in Brazil in the 1990s, the B biotype of silverleaf whitefly (Bemisia tabaci Genn., Hemiptera: Aleyrodidae has been recognized as an important pest in soybeans (Glycine max L., reducing the productivity of this legume species in some areas of the country. As an alternative to chemical control, the use of resistant genotypes represents an important tool for integrated pest management (IPM. This study evaluated the performance of 10 soybean genotypes prior to whitefly infestation, by testing attractiveness and preference for oviposition in the greenhouse and antibiosis in the laboratory. In a multiple-choice test, 'IAC-17' was the least attractive to insects. In a no-choice test, 'IAC-17' was the least attractive for egg deposition, indicating the occurrence of non-preference for oviposition on this genotype. Trichome density was positively correlated with the oviposition site and may be associated with the resistance of 'IAC-17' to infestation. The genotypes 'IAC-PL1', 'IAC-19', 'Conquista', 'IAC-24' and 'IAC-17' extended the insect's life cycle, indicating the occurrence of a small degree of antibiosis and/or non-preference for feeding.Desde que se registró por primera vez en Brasil en la década de 1990, el biotipo B de la mosca-blanca (Bemisia tabaci Genn., Hemiptera: Aleyrodidae, se reconoce como una importante plaga de la soya (Glycine max L. y es lo que reduce la productividad de estas especies de leguminosas en algunas zonas del pais. Como una alternativa al control químico, el uso de genotipos resistentes representa una herramienta importante para la gestión integrada de plagas (MIP. Este trabajo evaluó el comportamiento de 10 genotipos de soya frente al ataque de la mosca-blanca, por medio de ensayos de atractividad y preferencia para ovipostura en invernaderos y antibiosis en laboratorio. En una prueba de elección multiple, 'IAC-17' fue el menos atractivo para los insectos. En una prueba sin elecci

  19. Comparison of fecundity and survival of hemlock woolly adelgid (Hemiptera: Adelgidae) in northern and southern populations

    Science.gov (United States)

    Artemis Roehrig; Joseph. Elkinton

    2011-01-01

    The hemlock woolly adelgid (HWA), Adelges tsugae Annand (Hemiptera: Adelgidae), is an introduced species first reported in the eastern United States in 1951. The infestation has since spread in all directions from its initial sighting in Virginia, to its current range from northern Georgia, to southern Maine, and westward into Tennessee, causing...

  20. DNA barcode development for three recent exotic whitefly (Hemiptera: Aleyrodidae) invaders in Florida

    Science.gov (United States)

    Several new whitefly species have become established in Florida in the past decade. Three, in particular, have caused noticible damage to residential plants in the landscape such as ficus hedges, palms, and bird of paradise. These are fig whitefly (FW), rugose spiraling whitefly (RSW), and Bondar’s ...

  1. Characterization of Antixenosis in Soybean Genotypes to Bemisia tabaci (Hemiptera: Aleyrodidae) Biotype B.

    Science.gov (United States)

    Baldin, E L L; Cruz, P L; Morando, R; Silva, I F; Bentivenha, J P F; Tozin, L R S; Rodrigues, T M

    2017-08-01

    Bemisia tabaci biotype B (Gennadius) is one of the most important soybean pest worldwide. Herein, 15 soybean genotypes were evaluated, to characterize the occurrence of antixenosis to B. tabaci biotype B. Initially, a multiple-choice test with all genotypes was carried out, evaluating the settling and oviposition preference at 3 d after infestation, and the colonization by nymphs after 48 d of infestation. Subsequently, a no-choice test, using 14 genotypes, was conducted with infested plants individually, and the number of eggs was counted after 72 h. Then, 10 genotypes were selected (indicative of resistance and susceptibility), which were evaluated for whitefly settling 24, 48, and 72 h after infestation and for oviposition 72 h after infestation. The trichomes of the leaflets were characterized for density, size, and inclination to establish possible correlations with the settling and oviposition in the genotypes. In the first multiple-choice test, involving 15 genotypes, 'IAC-17,' 'IAC-19,' and UX-2569-159 expressed antixenosis against B. tabaci. 'Jackson,' 'P98Y11,' and PI-229358 exhibited the same behavior in the no-choice test. In the multiple-choice test, 'Jackson,' 'P98Y11,' and 'TMG1176 RR' were the least attractive and least used for oviposition. The antixenosis shown by 'Jackson,' 'P98Y11,' and PI-229358 may be related to the characteristics of the trichomes (lower density and inclined). Based on the experiments carried out, 'IAC-17,' 'IAC-19,' 'Jackson,' 'P98Y11,' PI-229358, TMG1176 RR, and UX-2569-159 are considered promising for resistance to B. tabaci biotype B and may be exploited in soybean breeding programs for resistance to insects. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  2. Quantifying conservation biological control for management of Bemisia tabaci (Hemiptera: Aleyrodidae) in cotton

    Science.gov (United States)

    Conservation biological control can be an effective tactic for minimizing insect-induced damage to agricultural production. The most effective manner of applying CBC is through an Integrated Pest Management (IPM) strategy, combining many tactics including cultural controls, pest sampling, the use of...

  3. Six New Record Species of Whiteflies (Hemiptera: Aleyrodidae) Infesting Morus alba in China

    Science.gov (United States)

    Wang, Ji-Rui; Song, Zao-Qin; Du, Yu-Zhou

    2014-01-01

    Abstract To determine the species of whiteflies occurring on mulberry, Morus alba L. (Rosales: Moraceae) in China, we collected samples in more than 87 sites in 16 provinces of China from 2008 to 2011. In total, 10 species, representing seven genera of the subfamily Aleyrodinae, were identified. Of these, six species are newly recorded on mulberry in China, namely, Aleuroclava ficicola Takahashi, Aleuroclava gordoniae (Takahashi), Aleurotrachelus camelliae (Kuwana), Bemisia afer (Priesner & Hosny), Bemisia tabaci Gennadius, and Pealius machili Takahashi. Information on the taxonomy, distribution, and host plants of the whitefly species found on mulberry in China, along with a brief description and illustrations of each species are provided. PMID:25368095

  4. Toxicity of insecticides to the sweetpotato whitefly (Hemiptera: Aleyrodidae) and its natural enemies.

    Science.gov (United States)

    Bacci, Leandro; Crespo, André L B; Galvan, Tederson L; Pereira, Eliseu J G; Picanço, Marcelo C; Silva, Gerson A; Chediak, Mateus

    2007-07-01

    Efficient chemical control is achieved when insecticides are active against insect pests and safe to natural enemies. In this study, the toxicity of 17 insecticides to the sweetpotato whitefly, Bemisia tabaci (Gennadius), and the selectivity of seven insecticides to natural enemies of this insect pest were evaluated. To determine the insecticide toxicity, B. tabaci adults were exposed to abamectin, acephate, acetamiprid, cartap, imidacloprid, malathion, methamidophos, bifenthrin, cypermethrin, deltamethrin, esfenvalerate, fenitrothion, fenpropathrin, fenthion, phenthoate, permethrin and trichlorphon at 50 and 100% of the field rate (FR), and to water (untreated control). To determine the insecticide selectivity, adults of Encarsia sp., Acanthinus sp., Discodon sp. and Lasiochilus sp. were exposed to abamectin, acephate, acetamiprid, cartap, imidacloprid, malathion and methamidophos at 50 and 100% FR, and to water. Groups of each insect species were exposed to kale leaves preimmersed in each treatment under laboratory conditions. Mortality of exposed individuals was recorded 24 h after treatment. Cartap and imidacloprid at 50 and 100% FR and abamectin and acetamiprid at 100% FR showed insecticidal activity to B. tabaci adults. Abamectin at 50 and 100% FR was the least insecticidal compound to the natural enemies Acanthinus sp., Discodon sp. and Lasiochilus sp. The present results suggest that abamectin at 100% FR may decrease B. tabaci field populations but can still be harmless to predators. Implications of these results within an integrated pest management context are discussed. Copyright (c) 2007 Society of Chemical Industry.

  5. CHEMICAL COMPOSITION AND TOXICITY OF CITRUS ESSENTIAL OILS ON Dysmicoccus brevipes (HEMIPTERA: PSEUDOCOCCIDAE)

    OpenAIRE

    MARTINS, GISELE DOS SANTOS OLIVEIRA; ZAGO, HUGO BOLSONI; COSTA, ADILSON VIDAL; ARAUJO JUNIOR, LUIS MOREIRA DE; CARVALHO, JOSÉ ROMÁRIO DE

    2017-01-01

    ABSTRACT The insect Dysmicoccus brevipes (Hemiptera: Pseudococcidae) has been reported as an important pest for several crops, especially coffee. The citrus essential oils can be obtained as by-products of the citrus-processing industry and have been tested as an alternative to control different insect groups. Therefore, the objective of this work was to determine the chemical composition and evaluate the toxicity of commercial sweet orange (Citrus sinensis), bitter orange (Citrus aurantium) ...

  6. Alguns aspectos da biologia de Triatoma pseudomaculata Corrêa & Espínola, 1964, em condições de laboratório (Hemiptera:Reduviidae:Triatominae

    Directory of Open Access Journals (Sweden)

    Teresa Cristina M Gonçalves

    1997-03-01

    Full Text Available Biology of Triatoma pseudomaculata Corrêa & Espínola, 1964, under Laboratory Conditions (Hemiptera:Reduviidae:Triatominae - Observations were made on the evolutive cycle of Triatoma pseudomaculata, held under laboratory conditions, fed weekly on bird (pigeon. Of 60 eggs obtained, only 34 nymphs reached the adult stage in a period of X(S=398±76 days. The following parameters were observed: the time immature stages took to develop from egg to adult emergence; the occurrence of the first meal; the time-lapse between the presenting of the blood-meal and the begining of feeding; time of feeding; amount of blood ingested; variation of weight 24 hr after the blood-meal and until the next blood-meal; and the defecation pattern. The experiment was carried out for 20 months, held in BOD incubator with the average of temperature and humidity of 28±1ºC and 80±5% RU, respectively

  7. Description of a new coccid (Hemiptera, Coccidae on avocado (Persea americana Mill. from Colombia, South America

    Directory of Open Access Journals (Sweden)

    Takumasa Kondo

    2010-04-01

    Full Text Available A new soft scale insect, Bombacoccus aguacatae Kondo, gen. n. and sp. n. (Hemiptera: Coccidae collected on the branches and twigs of avocado, Persea americana Mill. (Lauraceae in Colombia, is described and illustrated based on the adult female. An updated taxonomic key to closely related genera of the Toumeyella-group is provided.

  8. Asociaciones áfido-parasitoide (Hemiptera: Aphididae; Hymenoptera: Braconidae, Aphidiinae en cultivos hortícolas orgánicos en Los Cardales, Buenos Aires, Argentina Aphid-parasitoid associations (Hemiptera: Aphididae; Hymenoptera: Braconidae, Aphidiinae on organic vegetable crops in Los Cardales, Buenos Aires, Argentina

    Directory of Open Access Journals (Sweden)

    Andrea V. Andorno

    2007-07-01

    Full Text Available Diez especies de áfidos (Hemiptera: Aphididae se hallaron parasitados por siete especies de parasitoides (Hymenoptera: Braconidae, Aphidiinae en cultivos hortícolas orgánicos. Myzus persicae (Sulzer fue el áfido más frecuentemente encontrado sobre una amplia variedad de cultivos, y con mayor diversidad de parasitoides asociados. Aphidius colemani Viereck fue el afidiino más usual, que ataca varias especies de áfidos. Ocho asociaciones tritróficas, involucrando Aphidius matricariae Haliday, han sido registradas por primera vez para la Argentina.Ten aphid species (Hemiptera: Aphididae were found parasitized by seven aphid parasitoid species (Hymenoptera: Braconidae, Aphidiinae on organic vegetable crops. Myzus persicae (Sulzer was the most frequent aphid found on a wide variety of crops, with the largest parasitoid diversity associated. Aphidius colemani Viereck was the most frequent aphidiine attacking several species of aphids. Eight tritrophic associations involving Aphidius matricariae Haliday are reported for the first time for Argentina.

  9. Influence of trap color on collection of the recently introduced Bean Plataspid, Megacopta cribraria (Hemiptera: Plataspidae).

    Science.gov (United States)

    Scott Horn; James Hanula

    2011-01-01

    Large numbers of the exotic bean plataspid, Megacopta cribraria (F.) (Hemiptera: Plataspidae). were first collected from several northeast Georgia counties beginning in October 2009 (Suiter and Ames 2009, Statewide Pest Alert). How this insect arrived in the United States and where it came from is still not known. The native range of M. cribraria is reported to be...

  10. Spotted alfalfa aphid, Therioaphis trifolii (Monell) (Hemiptera: Aphididae): Pest on alfalfa in Serbia

    OpenAIRE

    Jovičić, Ivana; Radonjić, Anđa; Petrović-Obradović, Olivera

    2017-01-01

    Spotted alfalfa aphid Therioaphis trifolii (Monell) (Hemiptera, Aphididae) is one of the most important alfalfa pest on the world. Also, it is the most abundant alfalfa aphid in Serbia. This aphid cause damage to alfalfa directly by feeding and indirectly by vectoring plant-pathogenic viruses. Some notes of morphology, host plants, damage, biology, vector role and distribution of spotted alfalfa aphid are given. Abundance of this aphid on alfalfa, influence of climates changes on its abundanc...

  11. Biological parameters of the non-target pest Aphis gossypii Glover ...

    African Journals Online (AJOL)

    Thiago Mota

    2013-04-17

    Apr 17, 2013 ... Development and commercial use of Bollgard cotton in the USA nearly promises versus today reality. Plant. J. 27:489-501. Pessoa LGA, Souza B, Carvalho CF, Silva M (2004). Aspectos da biologia de Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) em quatro cultivares de Algodoeiro, em Laboratório.

  12. Pengaruh Jenis Mangsa dan Suhu pada Perkembangan Menochilus sexmaculatus Fabricius (Coleoptera: Coccinellidae dan Peranannya dalam Pengendalian Diaphorina citri Kuwayama (Hemiptera: Psyllidae

    Directory of Open Access Journals (Sweden)

    Tris Haris Ramadhan

    2008-07-01

    Full Text Available Diaphorina citri Kuwayama (Hemiptera: Psyllidae is the vector of citrus greening (Huanglongbing bacterium and the most serious impediment to citrus culture. Classical biological control of this psyllid vector should contribute to suppress their population. This research was conducted to determine the performance of Menochilus sexmaculatus Fabricius (Coleoptera: Coccinellidae when they were fed with D. citri. The larval performance index of M. sexmaculatus on D. citri compared with Aphis craccivora Koch (Hemiptera: Aphididae diet was 1.3.M. sexmaculatus fed with D. citri had lower fitness than those fed with A. craccivora as shown by longer larval stadium, lower adult dry weight, less number of egg produced and lower percentage of egg hatched. M. sexmaculatus grew best at the temperature of 27oC. Employing the exclusion procedure under field condition,M. sexmaculatus could reduce the population of D. citri up to 90%. These findings showed that theM. sexmaculatus could be a potential predator in reducing D. citri, particularly when the more preferred prey A. craccivora was not present.   Diaphorina citri Kuwayama (Hemiptera: Psyllidae merupakan kelompok Psyllid yang menularkan penyebab penyakit Huanglongbing yang sangat berbahaya pada tanaman jeruk. Pengendalian hayati klasik telah banyak memberikan kontribusi dalam pengendalian di lapangan. Penelitian ini dilakukan untuk melihat penampilan Menochilus sexmaculatus Fabricius (Coleoptera: Coccinellidae dengan pakan D. citri. Indeks penampilan larva M. sexmaculatus dengan pakan D. citri dibandingkan dengan Aphis craccivora Koch (Hemiptera: Aphididae adalah 1,3. M. sexmaculatus yang diberi pakan D. citri menunjukkan penurunan kebugaran dibandingkan jika diberi pakan A. craccivora seperti yang ditunjukkan dengan stadium larva lebih lama, penurunan berat kering serangga dewasa, telur yang dihasilkan lebih sedikit, dan penurunan jumlah telur yang menetas. Menggunakan metode eksklusi pada kondisi

  13. Host range of the exotic brown marmorated stink bug, Halyomorpha halys, (Hemiptera: Pentatomidae), implications for future distribution

    Science.gov (United States)

    Gary Bernon; Karen M. Bernhard; Anne L. Nielsen; James F. Stimmel; E. Richard Hoebeke; Maureen E. Carter

    2007-01-01

    Halyomorpha halys, (Hemiptera: Pentatomidae), is a pest in eastern Asia on soybeans and woody plants, including broadleaved trees and fruit trees. A population was discovered in Allentown, PA in 2001. H. halys is also a nuisance pest as it overwinters in homes and other buildings. Based on earlier reports to the Lehigh County...

  14. Population Dynamics of Empoasca fabae (Hemiptera: Cicadellidae) in Central Iowa Alfalfa Fields

    Science.gov (United States)

    Weiser Erlandson, L. A.; Obrycki, J. J.

    2015-01-01

    Adults and nymphs of Empoasca fabae Harris (Hemiptera: Cicadellidae) and adults of predatory species in the families Coccinellidae, Anthocoridae, Nabidae, Chrysopidae, and Hemerobiidae were sampled in Iowa alfalfa fields from June to September in 1999 and 2000. The relationship between each predatory taxa and E. fabae was examined using regression analysis. In 2000, all predators were found to be positively correlated with the presence of E. fabae during all periods sampled and most likely contributed to mortality. Orius insidiosus (Say) (Hemiptera: Anthoridae) was the most numerous insect predatory species; population numbers ranged from 0 to 1 and 0.1 to 3.7 adults per 0.25 m2 in 1999 and 2000, respectively. Partial life tables were constructed for E. fabae nymphs for two alfalfa-growing periods. Nymphs were grouped into three age intervals: first and second, third and fourth, and fifth instars. For the first alfalfa growing period examined, E. fabae nymphal mortality was 70% in 1999 and 49% in 2000. During the last growing period of each season (August–September), total nymphal mortality was relatively low (<25%). Adult E. fabae density ranged from 5.4 to 25.6 and 1.4–9.2 per 0.25 m2 in 1999 and 2000, respectively. E. fabae population peaks were similar for each age interval in all growing periods. This study provides further information on the population dynamics of E. fabae and its relationship with select predatory species in Iowa alfalfa fields. PMID:26320260

  15. Prey suitability and phenology of Leucopis spp. (Diptera: Chamaemyiidae) associated with hemlock woolly adelgid (Hemiptera: Adelgidae) in the Pacific Northwest

    Science.gov (United States)

    Sarah M. Grubin; Darrell W. Ross; Kimberly F. Wallin

    2011-01-01

    Leucopis spp. (Diptera: Chamaemyiidae) from the Pacific Northwest previously were identified as potential biological control agents for the hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae), in the eastern United States. We collected Leucopis spp. larvae from A. tsugae...

  16. Monitoring of brown stink bug (Hemiptera: Pentatomidae) population dynamics in corn to predict its abundance using weather data

    Science.gov (United States)

    The brown stink bug (BSB), Euschistus servus (Say) (Hemiptera: Pentatomidae), is a serious economic pest of corn production in the Southeastern U. S. The BSB population dynamics was monitored for 17 wks from tasseling to pre-harvest of corn plants (i.e., late May to mid-September) using pheromone ...

  17. Identification of a sex pheromone of the chrysanthemum lace bug Corythucha marmorata (Hemiptera: Tingidae)

    OpenAIRE

    Watanabe, Kisaki; Shimizu, Nobuhiro

    2017-01-01

    Although the nymphs of Corythucha marmorata form clusters on the undersides of host plant leaves, as frequently observed for Hemiptera, the adults are scattered in the vicinity of the nymph population. By investigating the biological activities of volatile secretions from the adult, we found that the secretions activated male mounting behaviour. A chemical analysis revealed that borneol was a common component of the secretions from both sexes. The absolute configuration of the natural product...

  18. Biological parameters and thermal requirements of the parasitoid Praon volucre (Hymenoptera: Braconidae) with Macrosiphum euphorbiae (Hemiptera: Aphididae) as host

    NARCIS (Netherlands)

    Conti, De B.F.; Bueno, V.H.P.; Sampaio, M.V.; Lenteren, van J.C.

    2011-01-01

    The effect of temperature on the biology of Praon volucre (Haliday, 1833) (Hymenoptera: Braconidae) in Macrosiphum euphorbiae (Thomas, 1878) (Hemiptera: Aphididae) hosts was studied and the thermal requirements of the parasitoid were determined. Experiments were carried out at 16, 19, 22, 25, and 28

  19. Brochosome influence on parasitisation efficiency of Homalodisca coagulata (Say) (Hemiptera: Cicadellidae) egg masses by Gonatocerus ashmeadi Girault (Hymenoptera : Mymaridae)

    NARCIS (Netherlands)

    Velema, H.P.; Hemerik, L.; Hoddle, M.S.; Luck, R.F.

    2005-01-01

    1. Many cicadellid females in the tribe Proconiini (Hemiptera: Cicadellidae) cover their egg masses with specialised, usually rod-shaped, brochosomes as the eggs are being laid. The brochosomes are produced in Golgi complexes in the Malpighian tubules of Cicadellidae. In contrast to the gravid

  20. Occurrence of the Tamarix Leafhopper, Opsius stactogalus Fieber (Hemiptera: Cicadellidae), in Argentina.

    Science.gov (United States)

    Virla, Eduardo G; Logarzo, Guillermo A; Paradell, Susana L

    2010-01-01

    The paleartic tamarix leafhopper, Opsius stactogalus Fieber (Hemiptera: Cicadellidae), can reduce the growth of tamarisk due to the aggregate feeding imposed by their populations. The species was mentioned for Argentina in Metcalf's catalogue (1967) without locality or region reference, and the contributions on Cicadellidae published by many authors after Metcalf omitted this distributional data. Populations of O. stactogalus on Tamarix sp. were found in 12 sites between 28 degrees 48' to 39 degrees 17' S and 64 degrees 06' to 70 degrees 04' W, located in both the Neotropical and Andean biogeographic regions.

  1. A review of Chinese tribe Achilini (Hemiptera: Fulgoromorpha: Achilidae), with descriptions of Paracatonidia webbeda gen. & sp. nov.

    Science.gov (United States)

    Long, Jian-Kun; Yang, Lin; Chen, Xiang-Sheng

    2015-12-02

    Planthoppers of the tribe Achilini (Hemiptera: Fulgoromorpha: Achilidae) from China, are reviewed. A key to the three genera of Chinese Achilini is given. A new genus and species of the tribe from southwestern China: Paracatonidia webbeda gen. & sp. nov., is described. A new genus and species record for China, Cixidia kasparyani Anufriev, is also given.

  2. Complex of primary and secondary parasitoids (Hymenoptera: Encyrtidae and Signiphoridae) of Hypogeoccoccus spp. mealybugs (Hemiptera: Pseudococcidae) in the New World

    Science.gov (United States)

    Parasitoids, both primary and secondary (hyperparasitoids), of Hypogeococcus spp. mealybugs (Hemiptera: Pseudococcidae) are reviewed to report results of the surveys in the New World conducted during 2009–2017 for perspective natural enemies of the Harrisia cactus mealybug, Hypogeococcus sp., which ...

  3. Population fluctuation of Empoasca sp. (Hemiptera: Cicadellidae in a physic nut crop in Mato Grosso do Sul

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    Denisar Paggioli de Carvalho

    2016-01-01

    Full Text Available Physic nut (Jatropha curcas L. is an oilseed, semi-evergreen shrub or small tree of the Euphorbiaceae family, whose seeds contain oil that can be processed into a high quality biofuel. However, there have been reports of arthropods feeding from its leaves, including the green leafhopper Empoasca sp. (Hemiptera: Cicadellidae. The large numbers of this insect, observed in certain periods of the year in many regions of Brazil, are causing damage to the oilseed crops. This study aims at evaluating the fluctuation in green leafhopper population in a physic nut crop in Dourados, Mato Grosso do Sul, to assess possible correlations with rainfall, maximum, average and minimum temperatures. This evaluation was conducted between March 2011 and July 2012. The largest Empoasca sp. populations were recorded in May and June, 2011, and between February and May, 2012. No significant correlation was observed between the weather parameters analyzed and the fluctuation in the Hemiptera population, but there was a trend toward higher population density during the warmer and rainier months.

  4. Prey Preference of Predatory mite Amblyseius swirskii (Athias-Henriot (Acari: Phytoseiidae on untreated and Beauveria bassiana-treated of Trialeurodes vaporariorum (Homoptera: Aleyrodidae

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    Marjan Seiedy

    2018-01-01

    Full Text Available Introduction The predator Amblyseius swirskii and entomopathogenic fungus Beauveria bassiana are important biocontrol agents of Trialeurodes vaporariorum. Determination of the host preference of predators in the fields when receiving signals related to either untreated and Beauveria bassiana-treated pest is important. Materials and Methods In this research, the prey preference of adult female (2 days old of A. swirskii was determined on untreated and Beauveria bassiana-treated of Trialeurodes vaporariorum (Homoptera: Aleyrodidae in various treatments base on Manly Index. These different treatments consisted of two time intervals; 24 and 48 h post-inoculation of greenhouse whiteflies with 1×105 conidia /ml of B. bassiana with 13 replicates. 24-h starved predators were added to the leaf discs singly then the number of consumed untreated and B. bassiana-treated T. vaporariorum in each Petri dish was assessed after 24 h. Results and Discussion The results revealed that mean number of consumed B. bassiana-treated T. vaporariorum in two time intervals; 24 and 48 h. after inoculation were 4/15±0/19 and 2.23±0/12, respectively. This investigation showed a significant preference of A. swirskii towards untreated T. vaporariorum in 24 (P< 0.0034 and 48 h. (P

  5. Effects of fertilization of four hemlock species on Adelges tsugae (Hemiptera: Adelgidae) growth and feeding preference of predators.

    Science.gov (United States)

    S.V. Joseph; James Hanula

    2011-01-01

    Understanding how fertilization affects host resistance to hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera:Adelgidae), is important because fertilizers are often used to grow resistant selections to a suitable size for testing. We evaluated four hemlock species (Tsuga) under three different fertilizer regimes to assess whether fertility affected resistance to...

  6. A new species of Tamarixia Mercet (Hymenoptera, Eulophidae, parasitoid of Trioza aguacate Hollis & Martin (Hemiptera, Triozidae in Mexico

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    Zoya Yefremova

    2014-01-01

    Full Text Available Tamarixia aguacatensis Yefremova, sp. n. (Hymenoptera: Eulophidae: Tetrastichinae is described from Mexico as a parasitoid of the avocado psyllid, Trioza aguacate Hollis & Martin (Hemiptera: Triozidae. Trioza aguacate is a serious pest of avocado, Persea americana Miller. A key to the species of Tamarixia Mercet in Mexico is given.

  7. Limited predation potential by adult and larval lady beetles (Coleoptera: Coccinellidae) on Nezara viridula (Hemiptera: Pentatomidae) eggs and nymphs

    Science.gov (United States)

    The Coccinellidae (Coleoptera) co-occur in many habitats with many arthropods that are of suitable size as prey. The Pentatomidae (Hemiptera) are one such group of insects with eggs and early instars that could be susceptible to predation by Coccinellidae. The objective of this laboratory study wa...

  8. Rapid Communication. Tamarixia monesus (Walker (Hym.: Eulophidae parasitoid of Bactericera tremblayi (Wagner, 1961 (Hemiptera: Triozidae in Iran

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    Lotfalizadeh Hossein

    2016-08-01

    Full Text Available Bactericera tremblayi (Wagner, 1961 (Hemiptera: Triozidae is reported on Brassica oleracea var. capitata (Brassicaceae in northwestern Iran. Tamarixia monesus (Walker (Hymenoptera: Chalcidoidea, Eulophidae was reared for the first time on B. tremblayi, and compared with Tamarixia tremblayi, another parasitoid of B. tremblayi. This is a new record of T. monesus from the Middle East.

  9. An unusual new species of Hallodapomimus Herczek, 2000 from the Eocene Baltic amber (Hemiptera, Heteroptera, Miridae, Phylinae

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    Aleksander Herczek

    2015-03-01

    Full Text Available Hallodapomimus antennatus sp. n. (Hemiptera: Heteroptera, Miridae, Phylinae, Hallodapini is described from a macropterous female found in Eocene Baltic amber. The new species can be recognized readily from the other species of the genus, mainly due to its unusual second antennal segment. A key for the identification of all known fossil Hallodapini is presented.

  10. Portanus Ball: descrição de uma espécie nova (Hemiptera, Cicadellidae, Xestocephalinae

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    Adenomar Neves de Carvalho

    2004-01-01

    Full Text Available Portanus dubius sp. nov. é descrita e ilustrada a partir de espécimens que foram coletados com armadilha Malaise durante um levantamento entomológico no Estado do Paraná, Brasil (PROFAUPAR.Portanus Ball: description of a new species (Hemiptera, Cicadellidae, Xestocephalinae. Portanus dubius sp. nov. is described and illustrated. The specimens were collected with Malaise trap during an entomological inventory in Paraná State, Brazil (PROFAUPAR.

  11. Scale insects (Hemiptera: Coccomorpha) on sugarcane in Colombia, with description of a new species of Tillancoccus Ben-Dov (Coccidae).

    Science.gov (United States)

    Caballero, Alejandro; Ramos-Portilla, Andrea Amalia; Kondo, Takumasa

    2017-05-02

    Herein we describe a new species, Tillancoccus koreguajae Caballero & Ramos, sp. n. (Hemiptera: Coccidae) from Colombia collected on sugarcane. Pinnaspis strachani (Cooley) is also recorded on sugarcane for the first time worldwide. An updated list of seven species of Coccomorpha on sugarcane in Colombia is provided, including information on its distribution, biology, and mutualistic ants for each species. Seven species of scale insects (Hemiptera: Coccomorpha) have been recorded previously on sugarcane, Saccharum officinarum L. (Poaceae) in Colombia: Pseudococcidae: Dysmicoccus boninsis (Kuwana), D. brevipes (Cockerell), Pseudococcus calceolariae (Maskell), Saccharicoccus sacchari (Cockerell); Coccidae: Pulvinaria elongata Newstead; Diaspididae: Duplachionaspis divergens (Green) and Serenaspis minima (Maskell). However, the record of S. minima in Colombia is considered doubtful as there are no voucher specimens from Colombia and because the distribution of this species is currently limited to the Australasian region. Pseudococcus calceolariae is present in Colombia but its record on sugarcane is also doubtful. A taxonomic key for the identification of scale insects on sugarcane in Colombia is provided.

  12. Thlastocoris laetus Mayr, 1866 (hemiptera: coreidae: acanthocerini) nueva plaga de la piña

    OpenAIRE

    Couturier,G.; Brailovsky,H.; Zucchi,R.A.

    1993-01-01

    Thlastocoris laetus (Hemiptera: Coreidae) es por primera vez señalado como plaga de la piña para la Amazonia brasileña y peruana comentando los daños que ocasiona. Se presenta la caracterización morfológica de esta especie, comparandola con Lybindus dichrous que también es reconocido como plaga de la piña en el Brasil. La distribución geográfica de ambas especies es citada.

  13. Factors affecting population dynamics of maternally transmitted endosymbionts in Bemisia tabaci.

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    Huipeng Pan

    Full Text Available While every individual of Bemisia tabaci (Hemiptera: Aleyrodidae harbors the primary symbiont (P-symbiont Portiera, the infection frequencies of the six secondary symbionts (S-symbionts including Hamiltonella, Arsenophonus, Cardinium, Wolbachia, Rickettsia and Fritschea vary greatly among different populations. To characterize the factors influencing the infection dynamics of the six S-symbionts in B. tabaci, gene-specific PCR were conducted to screen for the presence of the P-symbiont Portiera and the six S-symbionts in 61 (17 B and 44 Q biotypes field populations collected from different plant species and locations in China. All individuals of the 61 populations hosted the P-symbiont Portiera, but none of them harbored Arsenophonus and Fritschea. The presence and infection rates of Hamiltonella, Cardinium, Rickettsia, Wolbachia and their co-infections Rickettsia + Hamiltonella (RH, Rickettsia + Cardinium (RC, Hamiltonella + Cardinium (HC and Rickettsia + Hamiltonella + Cardinium (RHC varied significantly among the 61 field populations; and the observed variations can be explained by biotypes, sexes, host plants and geographical locations of these field populations. Taken together, at least three factors including biotype, host plant and geographical location affect the infection dynamics of S-symbionts in B. tabaci.

  14. First report of Idiopterus nephrelepidis Davis, 1909 (Hemiptera: Aphididae from Bulgaria

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    Elena Tasheva-Terzieva

    2011-11-01

    Full Text Available Idiopterus nephrelepidis (Hemiptera: Aphididae is reported for the first time in Bulgaria on ornamental ferns in four greenhouses in Sofia and Varna. Dense colonies of apterous viviparous females and larvae were observed. The established host plants are Nephrolepis exaltata, Asplenium nidus and Pteris cretica. Infested ferns exhibit leaf deformation. The aphids were reared in laboratory conditions for four months. A morphometric study of apterae was carried out. Taking into account the presence of host plants of I. nephrelepidis in Bulgaria which are native to the local flora and the reports of the aphid from the Balkan area, it may spread in the country outdoors.

  15. Flight Muscle Dimorphism and Heterogeneity in Flight Initiation of Field-Collected Triatoma infestans (Hemiptera: Reduviidae)

    OpenAIRE

    Gurevitz, Juan M.; Kitron, Uriel; Gürtler, Ricardo E.

    2007-01-01

    Recent experiments demonstrated that most field-collected Triatoma infestans (Klug) (Hemiptera: Reduviidae) adults from northern Argentina either never initiated flight or did so repeatedly in both sexes. This pattern could not be explained by sex, adult age, weight, weight-to-length ratio (W/L), or chance. We examined whether bugs that never initiated flight possessed developed flight muscles, and whether flight muscle mass relative to total body mass (FMR) was related to the probability of ...

  16. A new species in the genus Crisicoccus Ferris (Hemiptera: Coccomorpha: Pseudococcidae), with a key to Chinese species.

    Science.gov (United States)

    Zhang, Jiang-Tao; Wu, San-An

    2016-06-01

    A new mealybug, Crisicoccus ziziphus sp. nov. (Hemiptera: Coccomorpha: Pseudococcidae), collected on the leaves and twigs of Ziziphus jujuba (Rhamnaceae), is described from China. All the female developmental stages (adult, third-instar, second-instar and first-instar nymphs) are described and illustrated. Keys are provided to separate the female instars and to identify adult females of Crisicoccus species from China.

  17. Review of the genus Neotetricodes Zhang et Chen (Hemiptera: Fulgoromorpha: Issidae) with description of two new species.

    Science.gov (United States)

    Chang, Zhi-Min; Yang, Lin; Zhang, Zheng-Guang; Chen, Xiang-Sheng

    2015-12-11

    Two new species of the issid genus Neotetricodes Zhang et Chen (Hemiptera: Fulgoromorpha: Issidae): Neotetricodes longispinus Chang et Chen sp. nov. (China: Yunnan) and Neotetricodes xiphoideus Chang et Chen sp. nov. (China: Yunnan) are described and illustrated. The generic characteristic is redefined. A checklist and key to the species of the genus are provided. The female genitalia of the genus are firstly described.

  18. Rearing and Release of Megamelus scutellaris Berg (Hemiptera: Delphacidae) for Biological Control of Water hyacinth in 2015

    Science.gov (United States)

    2017-06-01

    Hemiptera: Delphacidae) for Biological Control of Waterhyacinth in 2015 by Jan Freedman and Nathan Harms PURPOSE: Waterhyacinth biological control ... control agents. Three insects were released in the United States for biological control of waterhyacinth during the 1970s; two weevils, Neochetina...content) and competitive interactions with other biological control agents (e.g., Neochetina spp.), though their consideration in other biological

  19. Biologia e tabela de vida de fertilidade do pulgão-preto em cultivares de videira

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    Cléber Antonio Baronio

    2014-09-01

    Full Text Available O objetivo deste trabalho foi avaliar a biologia e a tabela de vida de fertilidade do pulgão-preto da videira [Aphis illinoisensis (Hemiptera: Aphididae], em mudas das cultivares de Vitis labrusca Bordô, e de V. vinifera Cabernet Franc, Itália e Moscato Bianco. O experimento foi conduzido em 50 minigaiolas de confinamento, com um inseto em cada gaiola por tratamento, fixadas em mudas de videira mantidas em câmara de crescimento do tipo fitotron (a 25±1°C, umidade relativa de 75±10% e fotófase de 14 horas. Avaliaram-se diariamente a duração e a viabilidade ninfal, a fecundidade e a longevidade do período reprodutivo da espécie. O pulgão-preto da videira completou o ciclo biológico nas mudas das mencionadas cultivares, com duração da fase de ninfa de 7,9±0,3, 6,8±0,2, 6,2±0,2 e 6,7±0,2 dias, e viabilidade de 58, 82, 98 e 80% para 'Bordô', 'Cabernet Franc', 'Itália' e 'Moscato Bianco', respectivamente. 'Cabernet Franc' e 'Moscato Bianco' foram mais favoráveis ao desenvolvimento do pulgão-preto, com base na tabela de vida de fertilidade, com 51,3 e 55,6 descendentes por fêmea, por geração, respectivamente. 'Bordô' foi a menos adequada ao desenvolvimento do afídeo, com 12,55 descendentes por fêmea, por geração, o que indica resistência do tipo antibiose ou não preferência do inseto pela cultivar.

  20. Diaphorina citri (Kuwayama, 1907 and Tamarixia radiata (Waterson, 1922 in citrus crops of Cundinamarca, Colombia

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    Ebratt Ravelo Everth Emilio

    2011-12-01

    Full Text Available

    The presence of <em>Diaphorina citri em>Kuwayama (Hemiptera: Psyllidae and <em>Tamarixia radiata em>(Waterston (Hymenoptera: Eulophidae was recorded in citrus crops of Cundinamarca, Colombia. This work is to know the geographic distribution of <em>D. citri em>and an initial record of the parasitoid <em>T. radiata em>in citrus producing areas of this department.

  1. Occurrence of the Tamarix Leafhopper, Opsius stactogalus Fieber (Hemiptera: Cicadellidae), in Argentina

    Science.gov (United States)

    Virla, Eduardo G.; Logarzo, Guillermo A.; Paradell, Susana L.

    2010-01-01

    The paleartic tamarix leafhopper, Opsius stactogalus Fieber (Hemiptera: Cicadellidae), can reduce the growth of tamarisk due to the aggregate feeding imposed by their populations. The species was mentioned for Argentina in Metcalf's catalogue (1967) without locality or region reference, and the contributions on Cicadellidae published by many authors after Metcalf omitted this distributional data. Populations of O. stactogalus on Tamarix sp. were found in 12 sites between 28° 48′ to 39° 17′ S and 64° 06′ to 70° 04′ W, located in both the Neotropical and Andean biogeographic regions. PMID:20578887

  2. PRIMER REPORTE DE LA PRESENCIA DE Diaphorina citri (HEMIPTERA: LIVIIDAE EN MANABÍ

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    José Bernardo Navarrete Cedeño

    2016-12-01

    Full Text Available Diaphorina citri (Hemiptera: Liviidae, conocido como el psílido asiático de los cítricos, es considerada una plaga clave de la citricultura mundial, debido a que es vector de la bacteria “Candidatus Liberibacter”, agente causal de la enfermedad “Huanglongbing”, que tiene efectos letales sobre vegetales dentro de la familia Rutaceae. En este documento se reporta la presencia de D. citri infestando Murraya spp., un arbusto de la familia Rutaceae, en la zona urbana del cantón Portoviejo. Este es el primer reporte de la plaga en la provincia de Manabí-Ecuador

  3. Dopluise (Hemiptera: Coccoidea geassosieer met die wipstertmier, Crematogaster peringueyi Emery (Hymenoptera: Formicidae

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    Johannes H. Giliomee

    2015-02-01

    Full Text Available Neste van die wipstertmier, Crematogaster peringueyi (Hymenoptera: Formicidae, is op verskeie plekke langs die kus van die Wes-Kaap versamel. Die doel was om vas te stel watter dopluisagtiges (Hemiptera: Coccoidea in die neste in assosiasie met hierdie miere leef. Dopluise van drie families, naamlik die Pseudococcidae (witluise, Coccidae (sagtedopluise en Kerriidae (lakdopluise is in die neste gevind, almal bekend daarvoor dat hulle heuningdou afskei. Hierdie mutualistiese verhouding tussen die miere en dopluise, bekend as mirmekofilie, is fakultatief van aard. Die wipstertmier blyk ook nie spesifiek te wees wat betref die plant waarop hulle nes maak nie.

  4. Description of a new genus, Galgoria gen. nov. (Hemiptera: Cicadidae: Cicadinae: Leptopsaltriini: Leptopsaltriina).

    Science.gov (United States)

    Lee, Young June

    2016-05-10

    A new cicada genus, Galgoria gen. nov., is described with Tanna herzbergi Schmidt, 1932 (from southern China) as its type species, which is placed in the subtribe Leptopsaltriina Moulton, 1923 of the tribe Leptopsaltriini Moulton, 1923 in the subfamily Cicadinae Latreille, 1802 (Hemiptera: Cicadidae). Tanna herzbergi Schmidt, 1932 is transferred from Tanna Distant, 1905 to Galgoria gen. nov. to become Galgoria herzbergi (Schmidt, 1932) comb. nov. Tanna apicalis Chen, 1940 syn. nov. and Tanna pseudocalis Lei & Chou, 1997 syn. nov. are synonymized here with Galgoria herzbergi (Schmidt, 1932) comb. nov.

  5. Fungal flora of the digestive tract of Triatoma infestans (Hemiptera: Reduviidae from Argentina Flora fúngica de tractos digestivos en Triatoma infestans (Hemiptera: Reduviidae en Argentina

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    G. A. Marti

    Full Text Available A survey of the fungal microbiota of the digestive tract of Triatoma infestans (Klug (Hemiptera: Reduviidae adults was carried out. Insects captured in the field from different provinces in Argentina, as well as individuals reared in artificial colonies, were used for dissection. Axenic cultures of the fungal species were identified and were deposited with mycological collections at La Plata , Argentina. A total of 33 fungal species, with the exception of three that were mycelia sterilia, belonging to 11 genera were identified. Thirty two species belonged to Ascomycota (Eurotiomycetes and Sordariomycetes and one to Zygomycota (Zygomycetes. The genera with the greatest number of species were Penicillium (15, Aspergillus (5, and Cladosporium (2. Among the isolated fungi, some of the species were entomopathogenic or pathogens of humans and other animals.En el presente estudio se realizó un relevamiento de la flora fúngica microbiana en tractos digestivos de adultos de Triatoma infestans (Klug (Hemiptera: Reduviidae. Se disecaron insectos capturados del campo en diferentes provincias Argentinas, así como también se utilizaron individuos de una colonia artificial. Fueron realizados cultivos axénicos de las especies fúngicas aisladas, los que fueron identificados y luego depositados en las colecciones de hongos entomopatógenos del CEPAVE La Plata , Argentina. Fueron identificadas 33 especies fúngicas perteneciente a 11 géneros. Treinta y dos especies pertenecen a Ascomycota (Eurotiomycetes y Sordariomycetes y una a Zygomycota (Zygomycetes. Los géneros con mayor número de especies fueron Penicillium (15, Aspergillus (5, y Cladosporium (2. Entre los aislamientos fúngicos, algunas de las especies encontradas son entomopatogénicas o patógenas de humanos y otros animales.

  6. Biological aspects of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on six bean genotypes

    International Nuclear Information System (INIS)

    Oriani, Maria A. de G.

    2008-01-01

    The silverleaf whitefly is one of the most harmful pests that attack bean crops, mainly for extracting large quantities of phloem sap and transmitting the bean golden mosaic virus. Resistant germplasm plants can be an important method for controlling this pest. The biological aspects of Bemisia tabaci B biotype on bean (Phaseolus vulgaris) genotypes were evaluated. The tests were conducted under laboratory conditions, with the following genotypes: Arc 1, Arc 3s, Arc 5s, G13028, G11056 and Porrillo 70. The bean plants in a stage IV-1 were infested during one day with silverleaf white flies. Afterwards the eggs and nymphs were observed until adult emergence. Longevity and fecundity of emerged insects were also evaluated. The longest development time occurred for nymphs fed on Arc 3s genotype (26.5 days), following by G11056 (25.9 days) and G13028 (25.3 days). The development period was 5.5 days longer in Arc 3s when compared with Porrillo 70. Also, the wild genotypes Arc 3s and G11056 showed higher mortality rates (94.7% and 83.1%, respectively), which may suggest antibiosis and/or feeding non preference resistance type. For this reason, although longevity and fecundity were not influenced when the whitefly fed on resistant genotypes (Arc 3s, G11056, G13028 and Arc 5s), those genotypes can be used for bean breeding program towards B. tabaci B biotype. (author)

  7. Factors affecting oviposition of Bemisia tabaci (Genn.) biotype B (Hemiptera: Aleyrodidae) in sweet pepper

    International Nuclear Information System (INIS)

    Lima, Larissa C. de; Campos, Alcebiades R.

    2008-01-01

    Bemisia tabaci (Gen.) biotype B is considered a pest of economical importance for several vegetables. The oviposition behaviour of the while fly was evaluated in sweet pepper plants. The trials were carried out under greenhouse condition and in the Laboratory of Entomology of DEFERS/ UNESP, Campus of Ilha Solteira-SP, with the sweet pepper Magali-R genotype. The effect of plant age on the whitefly oviposition was evaluated in free-choice tests, in plants, 25, 30, 35, 40 and 45- day-old, as egg distribution in the plant and on the leaf blade was evaluated in 35-days-old plants. In a no-choice tests, 35-day-old plants were used to evaluate the effect of the densities of 50, 100, 150, 200 and 250 adults per plant on the number of eggs laid by insects. The silver leaf whitefly preferred to oviposition the third to sixth leaflets, of the medium and superior part of plants of sweet pepper; the leaf blade areas, located in the lobes right and left close the base of the leaf were the preferential site for whitefly oviposition. Older plants, 40- and 45-day-old, were preferentially used for oviposition, and 200 and 250 adults per plant were both enough to lay a number of eggs that allowed to differentiate among sweet pepper genotypes with different whitefly resistance levels. (author)

  8. Biology of the citrus blackfly, Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae), in three host plants

    International Nuclear Information System (INIS)

    Pena, Marcia R.; Silva, Neliton M. da

    2009-01-01

    The citrus blackfly, Aleurocanthus woglumi Ashby, was detected in Brazil in 2001. The aim of this research was to evaluate the biology, biometry and host preference of A. woglumi in sweet orange, acid lime Tahiti and mango. Experiments were set in laboratory conditions with insects collected in rangpur lime plants in Manaus, State of Amazonas, from January to June of 2006. The following parameters were evaluated: number of spirals (ovo positions) and eggs per plant, number of eggs by spiral per plant, survival of the immature (eggs, 1st, 2nd, 3rd and 4th instars), and length and survival of the immature stage. The mean embrionary period was 15 days for the three hosts. The 4th nymph (puparium) was the longest during nymph development. Second and third instars had the highest survival. The mean length of the egg-adult cycle was 70 days for the three hosts evaluated. The eggs were laid in a spiral shape on the adaxial leaf surface. The 1st instars moved to short distances from the spiral, while the 2nd, 3rd and 4th are sessile and have bristles on the whole body. Based on the highest oviposition and the highest survival of the immature stage of the citrus blackfly in acid lime Tahiti, this plant can be considered the most suitable host to A. woglumi. (author)

  9. The identity and distribution of Fiorinia phantasma (Cockerell & Robinson) (Hemiptera: Coccomorpha: Diaspididae), with a new synonym.

    Science.gov (United States)

    Watson, Gillian W; Williams, Douglas J; Miller, Douglass R

    2015-11-25

    The morphologies of Fiorinia phantasma (Cockerell & Robinson) (Hemiptera: Coccomorpha: Diaspididae) and F. coronata Williams & Watson are reviewed, and the name F. coronata is placed as a junior synonym of the name F. phantasma syn. n. The known geographical distribution and host range of F. phantasma is documented and discussed. An identification key to 12 of the 16 species of Fiorinia known from the Australasian, Nearctic and Neotropical Regions is provided.

  10. Scale Insects (Hemiptera: Coccoidea) on Myrciaria dubia (Myrtaceae) in Brazil.

    Science.gov (United States)

    Wolff, V R S; Kondo, T; Peronti, A L B G; Noronha, A C S

    2016-06-01

    Commercial cultivation of the fruit tree Myrciaria dubia (Myrtaceae) is being developed in Brazil but phytophagous insects, including scale insects (Hemiptera: Coccoidea), can become pests in plantations. The coccids Ceroplastes jamaicensis White, Coccus viridis (Green), Parasaissetia nigra (Nietner), Pseudokermes vitreus (Cockerell) (Coccidae), and the diaspidid Pseudaonidia trilobitiformis (Green) were collected on M. dubia in the municipality of Belém and Tomé-Açu, state of Pará (PA), metropolitan and Northeast Pará mesoregions, Brazil. A key to species of Coccoidea recorded on M. dubia, based on adult females, is provided. Photographs for all scale insects reported on M. dubia are provided. Ceroplastes jamaicensis is recorded for the first time for Brazil and is herein reported for the first time associated with this host.

  11. New corological and biological data of the Red Gum Lerp Psyllid, Glycaspis brimblecombei Moore, 1964 in Italy (Hemiptera, Psyllidae

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    Ricardo Jiménez-Peydró

    2011-03-01

    Full Text Available Glycaspis brimblecombei Moore, 1964 is a psyllid (Hemiptera: Psyllidae pest of Eucalyptus, native toAustralia and first recorded in Europe: Spain in 2008 and more recently (2010 in Italy. The present paper dealswith recent research, carried out in central Italy, with new data on the distribution and biology of this species.

  12. Characterization of a recombinant Cathepsin B-Like cysteine peptidase from Diaphorina citri Kuwayama (Hemiptera: Liviidae): A putative target control of citrus huanglongbing

    Science.gov (United States)

    Huanglongbing (HLB) is one of the most destructive disease affecting citrus plants. The causal agent is associated with the phloem-limited bacterium Candidatus Liberibacter asiaticus (CLas) spread by the Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae). Among the control strategies for H...

  13. Occurrence of lace bug Vatiga illudens and Vatiga manihotae (Hemiptera: Tingidae) in Mato Grosso do Sul, midwestern Brazil.

    Science.gov (United States)

    Bellon, Patrícia P; Wengrat, Ana P G S; Kassab, Samir O; Pietrowski, Vanda; Loureiro, Elisângela S

    2012-09-01

    Nymphs and adults of the lace bug (Hemiptera: Tingidae) have been found in cassava crops (Manihot esculenta) in Mato Grosso do Sul State, Brazil. The insects were collected in the field and taken to the laboratory where they were identified based on some morphological traits of the species Vatiga manihotae (Drake) and V. illudens (Drake), which are first reported in the aforementioned state.

  14. Rodolia cardinalis (Mulsant) (Coleoptera: Coccinellidae), a new predator of Crypticerya multicicatrices Kondo and Unruh (Hemiptera: Monophlebidae)

    OpenAIRE

    Pinchao, Ever Camilo; Kondo, Takumasa; González F., Guillermo

    2016-01-01

    The Colombian fluted scale (CFS), Crypticerya multicicatrices Kondo & Unruh (Hemiptera: Monophlebidae) is a polyphagous scale insect which affects about 100 species of plants. Between 2010–2013, the species was reported as an invasive pest on the islands of San Andres and Old Providence, Colombian territory in the Caribbean sea. Currently, populations of the CFS also have increased in the city of Cali, Valle del Cauca State, mainland Colombia, affecting different host plants, especially l...

  15. PCR-based gut content analysis to detect predation of Eriococcus ironsidei (Hemiptera: Eriococcidae) by Coccinellidae species in macadamia nut orchards in Hawaii

    Science.gov (United States)

    Macadamia felted coccid, Eriococcus ironsidei (Williams) (Hemiptera: Eriococcidae) was first found infesting macadamia trees in the island of Hawaii in 2005. Macadamia felted coccid infests all above-ground parts of trees to feed and reproduce. Their feeding activity distorts and stunts new growth w...

  16. A new species of Dysmicoccus damaging lavender in French Provence (Hemiptera, Sternorrhyncha, Pseudococcidae).

    Science.gov (United States)

    Germain, J-F; Matile-Ferrero, D; Kaydan, M B; Malausa, T; Williams, D J

    2015-07-01

    Une nouvelle espèce de Dysmicoccus nuisible à la lavande en Provence (France) (Hemiptera, Sternorrhyncha, Pseudococcidae). Dysmicoccus lavandulae Germain, Matile-Ferrero & Williams n. sp. est décrite et illustrée. Ses séquences ADN sont présentées. L'espèce vit sur Lavandula x intermedia cultivée pour la production d'essence de lavande en Provence. La liste des espèces de pseudococcines vivant sur les lavandes spontanées en France est dressée. Le statut des 2 genres voisins Trionymus Berg et Dysmicoccus Ferris est discuté.

  17. Ecological aspects of a Cosmopolites sordidus (Germar (Coleoptera: Curculionidae population in São Miguel do Iguaçu, PR./ Aspectos ecológicos da população de Cosmopolites sordidus, (Germar (Coleoptera: Curculionidae em São Miguel do Iguaçu, PR

    Directory of Open Access Journals (Sweden)

    Cristiane Rohde

    2006-06-01

    Full Text Available The population dynamics of Cosmopolites sordidus (Germar was studied in banana crops (cultivar Nanicão at São Miguel do Iguaçu, PR, using roof-tile-like baits, between June/2003 and May/2004. Adults infected by entomopathogenic fungi were recorded, and predators were also found on the baits. A comparative bioassay was carried out between the Beauveria bassiana isolate obtained from the experiment area and from other regions in Brazil against C. sordidus adults. The population peaks of C. sordidus adults occurred in June and July, a period with mild temperatures and low precipitation. The population of C. sordidus larvae, however, was concentrated in December. The insect predators found in the area were insects of the following orders: Coleoptera (Carabidae, Dermaptera (Forficulidae, Hemiptera (Reduviidae, Hymenoptera (Formicidae, and spiders of the families Ctenidae, Clubionidae, and Lycosidae, with a population peak two months after the pest population peak. A low number of C. sordidus adults infected by B. bassiana was found, with an annual mean of 0.44%. In the laboratory, the B. bassiana isolates were pathogenic to the insects.Estudou-se a dinâmica populacional de Cosmopolites sordidus (Germar em cultivo de banana(cultivar Nanicão, em São Miguel do Iguaçu, PR, através de iscas do tipo telha, entre junho/2003 a maio/2004. Atribuiu-se conceito ao estado de conservação da isca, visando observar sua durabilidade e atratividade. Registraram-se adultos infectados por fungos entomopatogênicos, sendo também encontrados predadores nas iscas. Realizou-se um bioensaio com isolados de Beauveria bassiana da área experimental e de Centros de Pesquisa do Brasil, para avaliar a patogenicidade em C. sordidus. Verificou-se que os picos populacionais de adultos de C. sordidus ocorreram em junho e julho, período de temperaturas amenas e baixas precipitações, enquanto que as larvas foram mais abundantes em dezembro. Os insetos predadores

  18. Primer registro de Triatoma infestans (Klug (Hemiptera: Reduviidae asociado a nidos de loros barranqueros (Cyanoliseus patagonus (Aves: Psittacidae

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    María L. HERNÁNDEZ

    2015-01-01

    Full Text Available Triatoma infestans es el vector más importante de la enfermedad de Chagas de la Argentina. Se cita por primera vez la presencia de T. infestans (Klug (Hemiptera: Reduviidae asociado a nidos de loro barranquero Cyanoliseus patagonus (Psittasiformes: Psittacidae construidos en viviendas de adobe abandonadas. Se discuten las posibles implicancias de este hallazgo para la vigilancia entomológica del vector.

  19. Population growth and within-plant distribution of the striped mealybug Ferrisia virgata (Cockerell (Hemiptera, Pseudococcidae on cotton

    Directory of Open Access Journals (Sweden)

    Martin D. Oliveira

    2014-03-01

    Full Text Available Population growth and within-plant distribution of the striped mealybug Ferrisia virgata (Cockerell (Hemiptera, Pseudococcidae on cotton. The striped mealybug, Ferrisia virgata (Cockerell (Hemiptera, Pseudococcidae, is a widely distributed and polyphagous pest species, which naturally occurs on cotton plants in Brazil. This study evaluated the establishment and population growth as well as the within-plant distribution of F. virgata on four cotton cultivars: CNPA 7H (white fibers, BRS Verde, BRS Safira, and BRS Rubi (colored fibers. The experiment was conducted in a complete randomized design with four treatments (cultivars and 18 replications of each. Thus, cotton plants of each cultivar were infested with 100 newly hatched nymphs of F. virgata. The number of adult female mealybugs and the total number of mealybugs per plant were quantified, respectively, at 25 and 50 days after infestation. The developmental and pre-reproductive periods were also determined. Furthermore, we verified the distribution of F. virgata on the plant parts at 25 and 50 days after infestation. Ferrisia virgata showed similar growth of 412-fold in the four cotton cultivars studied. Also, the nymphs were spread on infested leaves; the secondgeneration nymphs were spread and established in all plant parts. Our results characterize F. virgata as having much potential as an important cotton pest in Brazil.

  20. Description of the immature stages of the planthopper Lacertinella australis (Hemiptera: Delphacidae)

    Science.gov (United States)

    Batiz, M. F. Rossi; Marino de Remes Lenicov, A. M.; Hagedorn, Henry

    2014-01-01

    Abstract The five immature stages of the planthopper Lacertinella australis (Remes Lenicov and Rossi Batiz) (Hemiptera: Delphacidae: Saccharosydnini) are described and illustrated. The main characters that allowed us to distinguish the various stages were body size, number of tarsomeres and metatibial spines, and number of teeth on the spur. New biological data based on laboratory rearing and field observations showed that L. australis can carry out its biological cycle successfully on the graminaceous pampas grass ( Cortaderia spp. Stapf (Poales: Poaceae)). In addition, the efficient rearing in captivity, the high survivorship registered, and overwintering only on this host plant suggests that L. australis is a potential biocontrol agent of this invasive graminaceous weed. This study provides information about the immature stages , including a key for their identification, based on laboratory reared specimens and field observations. Resumen Se describen e ilustran las cinco etapas inmaduras de la especie de Saccharisydnini Lacertinella australis (Remes Lenicov and Rossi Batiz) (Hemiptera: Delphacidae). Los principales caracteres que permitieron distinguir las diferentes etapas fueron: tamaño corporal, número de espinas en los tarsómeros y metatibia y número de dientes en el espolón tibial. Nuevos datos biológicos, basados en la cría de laboratorio y observaciones de campo, mostraron que L. australis puede realizar su ciclo biológico exitosamente en la graminácea cortadera ( Cortaderia spp. Stapf (Poales: Poaceae)). Además, la eficiente crianza en cautive-rio y la alta supervivencia registrada en esta planta hospedera, sugieren que L. australis podría ser usada como un potencial agente de control biológico de esta maleza invasora. Este estudio proporciona informa-ción sobre las etapas inmaduras, incluyendo una clave para su identificación, basada en individuos provenientes de la cría de laboratorio y de campo. PMID:25199992

  1. A severity rating system for evaluating stand-level balsam woolly adelgid (Hemiptera: Adelgidae) damage in two Abies species in western North America

    Science.gov (United States)

    Kathryn H. Hrinkevich; Robert A. Progar; David C. Shaw

    2016-01-01

    Severity rating systems are fundamental to understanding the impacts of disturbance agents in forest stands. The balsam woolly adelgid (BWA), Adelges piceae (Ratzeburg) (Hemiptera: Adelgidae), is an invasive forest pest in North America that infests and causes mortality in true fir, Abies spp. There is currently no single...

  2. Natural parasitism of Diaphorina citri Kuwayama (Hemiptera, Psyllidae nymphs by Tamarixia radiata Waterston (Hymenoptera, Eulophidae in São Paulo orange groves Parasitismo natural de ninfas de Diaphorina citri Kuwayama (Hemiptera, Psyllidae por Tamarixia radiata Waterston (Hymenoptera, Eulophidae em pomares de laranja em São Paulo

    Directory of Open Access Journals (Sweden)

    Paulo Eduardo Branco Paiva

    2012-12-01

    Full Text Available The psyllid Diaphorina citri Kuwayama 1908 has become the main citrus pest species in the state of São Paulo, Brazil, after the introduction of the huanglongbing or citrus greening. This study evaluated the parasitism of 3rd, 4th and 5th instar D. citri nymphs by Tamarixia radiata (Waterston, 1922 in citrus groves under a regimen of regular insecticide applications in ten producing regions: Araraquara, Barretos, Bauru, Botucatu, Franca, Itapetininga, Jaú, Limeira, Lins and São João da Boa Vista. Sixty-nine samples of new branches infested with nymphs of D. citri were collected from 2005 to 2008 in orange groves ranging from 1 to 20 years old, of the varieties Hamlin, Pera, Valencia and Natal. The parasitoid T. radiata is widely distributed in São Paulo orange groves, and was identified in 50 (72% of the samples, showing a mean parasitism rate of 12.4%. The highest parasitism rate was observed in the "summer" (from January through March, with a mean of 25.7%. Nymphal parasitism was above 90% in two samples. The probable causes of the variations in parasitism of D. citri by T. radiata are discussed.O psilídeo Diaphorina citri Kuwayama 1908 tornou-se a principal praga dos citros no estado de São Paulo após a introdução do huanglongbing ou grenning dos citros. Este estudo avaliou as proporções de ninfas de terceiro a quinto ínstares de D. citri parasitadas por Tamarixia radiata (Waterston, 1922 em pomares de laranja submetidos a pulverizações constantes de inseticidas em dez regiões produtoras, Araraquara, Barretos, Bauru, Botucatu, Franca, Itapetininga, Jaú, Limeira, Lins e São João da Boa Vista. Foram coletadas 69 amostras de ramos jovens infestados com ninfas de D. citri obtidas entre 2005 e 2008 em pomares de 1 a 20 anos de idade, das cultivares Hamilin, Pera, Valencia e Natal. O parasitóide T. radiata encontra-se amplamente distribuído em pomares de São Paulo, tendo sido observado em 50 amostras (72%, com taxa média de

  3. Evaluation of <em>HER2em> Gene Amplification in Breast Cancer Using Nuclei Microarray <em>in em>S>itu em>Hybridization

    Directory of Open Access Journals (Sweden)

    Xuefeng Zhang

    2012-05-01

    Full Text Available Fluorescence<em> em>>in situ em>hybridization (FISH assay is considered the “gold standard” in evaluating <em>HER2/neu (HER2em> gene status. However, FISH detection is costly and time consuming. Thus, we established nuclei microarray with extracted intact nuclei from paraffin embedded breast cancer tissues for FISH detection. The nuclei microarray FISH (NMFISH technology serves as a useful platform for analyzing <em>HER2em> gene/chromosome 17 centromere ratio. We examined <em>HER2em> gene status in 152 cases of invasive ductal carcinomas of the breast that were resected surgically with FISH and NMFISH. <em>HER2em> gene amplification status was classified according to the guidelines of the American Society of Clinical Oncology and College of American Pathologists (ASCO/CAP. Comparison of the cut-off values for <em>HER2em>/chromosome 17 centromere copy number ratio obtained by NMFISH and FISH showed that there was almost perfect agreement between the two methods (κ coefficient 0.920. The results of the two methods were almost consistent for the evaluation of <em>HER2em> gene counts. The present study proved that NMFISH is comparable with FISH for evaluating <em>HER2em> gene status. The use of nuclei microarray technology is highly efficient, time and reagent conserving and inexpensive.

  4. Neonatal Phosphate Nutrition Alters <em>in em>Vivo> and <em>in em>Vitro> Satellite Cell Activity in Pigs

    Directory of Open Access Journals (Sweden)

    Chad H. Stahl

    2012-05-01

    Full Text Available Satellite cell activity is necessary for postnatal skeletal muscle growth. Severe phosphate (PO4 deficiency can alter satellite cell activity, however the role of neonatal PO4 nutrition on satellite cell biology remains obscure. Twenty-one piglets (1 day of age, 1.8 ± 0.2 kg BW were pair-fed liquid diets that were either PO4 adequate (0.9% total P, supra-adequate (1.2% total P in PO4 requirement or deficient (0.7% total P in PO4 content for 12 days. Body weight was recorded daily and blood samples collected every 6 days. At day 12, pigs were orally dosed with BrdU and 12 h later, satellite cells were isolated. Satellite cells were also cultured <em>in vitroem> for 7 days to determine if PO4 nutrition alters their ability to proceed through their myogenic lineage. Dietary PO4 deficiency resulted in reduced (<em>P> < 0.05 sera PO4 and parathyroid hormone (PTH concentrations, while supra-adequate dietary PO4 improved (<em>P> < 0.05 feed conversion efficiency as compared to the PO4 adequate group. <em>In vivoem> satellite cell proliferation was reduced (<em>P> < 0.05 among the PO4 deficient pigs, and these cells had altered <em>in vitroem> expression of markers of myogenic progression. Further work to better understand early nutritional programming of satellite cells and the potential benefits of emphasizing early PO4 nutrition for future lean growth potential is warranted.

  5. Four new species of the genus Mongoliana Distant (Hemiptera: Fulgoromorpha: Issidae) from southern China.

    Science.gov (United States)

    Meng, Rui; Wang, Yinglun; Qin, Daozheng

    2016-01-05

    Four new species in the planthopper genus Mongoliana Distant from southern China (Hemiptera: Fulgoromorpha: Issidae) are reported. Three of them, M. bistriata sp. nov., M. latistriata sp. nov. and M. albimaculata sp. nov., are described and illustrated; the fourth new one, M. arcuata sp. nov., is briefly described for M. triangularis Chen, Zhang & Chang which was a misidentification of M. triangularis Che, Wang & Chou. M. recurrens (Butler, 1875) is re-described and remarks for its current status is given. A key to all known species of Mongoliana is provided. The distribution and morphological peculiarities of the genus are briefly discussed.

  6. Studies on idiocerine leafhoppers with descriptions of Chinaocerus gen. nov. and three new species from China (Hemiptera: Cicadomorpha: Cicadellidae).

    Science.gov (United States)

    Dang, Li-Hong; Zhang, Bin; Li, Zi-Zhong

    2016-03-08

    A new leafhopper genus of the tribe Idiocerini (Hemiptera: Cicadomorpha: Cicadellidae), Chinaocerus, is described from the southwest China together with three new species, C. kangdingensis Zhang & Li sp. nov. (Sichuan Province), C. bispinatus Zhang & Li sp. nov. (Yunnan Province) and C. shii Zhang & Li sp. nov. (Sichuan Province). Descriptions and illustrations of these three new species are provided, and a key for their separation is also given.

  7. [Development of Rhodnius pictipes Stal, 1872 fed on mice and through a silicone membrane (Hemiptera, Reduviidae, Triatominae)].

    Science.gov (United States)

    Rocha, D da S; da Fonseca, A H; Costa, F A; Jurberg, J; Galvão, C

    1997-01-01

    Rhodnius pictipes (Hemiptera, Reduviidae) from Serra Norte, State of Pará, Brazil, acclimatized in an insectary at the Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Departamento de Entomologia, Instituto Oswaldo Cruz, were fed through a silicone membrane. In order to know the viability and the efficiency of this membrane compared with insects fed on mice, the number of bloodmeals taken, period of development of the five nymphal instars, longevity of adults, average amount of blood intake in each meal and percent of mortality were observed. A total of 310 insects, were used, comprising 50 nymphs of each instar, as well as 30 male and 30 female adults. Insects fed artificially had reduced minimal and maximal periods of development than the group fed on mice. The largest relative increase of body weight was observed in the 2nd instar followed by the 1st, and the amount of blood ingested increased during the development, to the 5th instar for both groups. There were no significant differences between the groups fed artificially and in vivo according to Tukey's test for p > 0.05. The percent of mortality in the 1st instar was 18% for artificially fed and 16% for the group fed on mice; these percentages decreased as insects developed until the 4th instar, without mortality, returning to increase in the 5th instar. R. pictipes was shown to be easily adaptable to artificial feeding, and could be considered as an important and viable experimental model.

  8. Do Scaphoideus titanus (Hemiptera: Cicadellidae) nymphs use vibrational communication?

    Science.gov (United States)

    Chuche, Julien; Thiéry, Denis; Mazzoni, Valerio

    2011-07-01

    Small Auchenorrhyncha use substrate-borne vibrations to communicate. Although this behaviour is well known in adult leafhoppers, so far no studies have been published on nymphs. Here we checked the occurrence of vibrational communication in Scaphoideus titanus (Hemiptera: Cicadellidae) nymphs as a possible explanation of their aggregative distributions on host plants. We studied possible vibratory emissions of isolated and grouped nymphs, as well as their behavioural responses to vibration stimuli that simulated presence of conspecifics, to disturbance noise, white noise and predator spiders. None of our synthetic stimuli or pre-recorded substrate vibrations from nymphs elicited specific vibration responses and only those due to grooming or mechanical contacts of the insect with the leaf were recorded. Thus, S. titanus nymphs showed to not use species-specific vibrations neither for intra- nor interspecific communication and also did not produce alarm vibrations when facing potential predators. We conclude that their aggregative behaviour is independent from a vibrational communication.

  9. Inheritance of sterility in Dysdercus koenigii F. (Hemiptera: Pyrrhocoridae)

    International Nuclear Information System (INIS)

    Harwalkar, M.R.; Rahalkar, G.W.

    1979-01-01

    It has been suggested that for the control of Lepidopterous populations, release of fully competitive partially sterile males would be more advantageous than the release of completely sterile males. This suggestion is based on the fact, elucidated in many studies, that partially sterilized males mated to normal females produce totally sterile or partially sterile progeny. In Hemiptera too, F 1 progeny of partially sterile males has been shown to inherit sterility. Individual impact of sterility inherited by either sex of the F 1 progeny on population growth has been studied in an hemipteran insect Dysdercus koenigii. A dose of 7 krad induces near-complete sterility in males. When males irradiated with substerilizing doses of 1,2 and 3 krad were crossed with normal females, F 1 progeny of both sexes was partially sterile : the female being more sterile than the males. When the F 1 progeny was intercrossed, there was enhanced reduction in progeny production. (auth.)

  10. Revision of the Neotropical treehopper genus Tolania (Hemiptera, Membracidae

    Directory of Open Access Journals (Sweden)

    Jesse L. Albertson

    2006-12-01

    ., T. corcula sp. nov., T. hamulata sp. nov., T. histria sp. nov., T. inornata sp. nov., T. laticornis sp. nov., T. melantha sp. nov., T. pogonia sp. nov., T. rideri sp. nov., and T. taura sp. nov. A key to the species groups and unplaced species is provided for Tolania males. The following species, previously placed in Tolania, are considered species incertae sedis: Cicada armata Stoll; Centrotus felinus Germar; Centrotus obscurus Germar; Centrotus fasciatus Walker. One new synonymy is proposed: Tolania humilis (Walker, 1858 = Tolania scutata Stål, 1862 syn. nov. Tolania cristata Lethierry sp. rev., is reinstated from synonymy with T. femoralis. Lectotypes are designated for T. cristata, T. femoralis, T. fraterna, and T. scutata.O gênero de soldadinhos Tolania Stål (Hemiptera, Membracidae, Nicomiinae, Nicomiini e 69 espécies válidas (59 novas espécies são descritos e ilustrados baseado na morfologia de adultos. Dez grupos informais de espécies são reconhecidos baseados em uma análise filogenética previamente publicada: (1 o grupo de espécies dira inclui T. calista sp. nov., T. dira sp. nov., T. inca sp. nov., T. macaria sp. nov., T. secoya sp. nov. e T. zaparo sp. nov.; (2 grupo fasciata inclui T. fasciata (Walker, T. gracilis sp. nov. e T. laticlava sp. nov.; (3 grupo femoralis inclui T. femoralis Stål, T. fraterna Stål e T. roberti sp. nov.; (4 grupo furcata inclui T. furcata sp. nov., T. tryphena sp. nov. e T. venezuelensis sp. nov.; (5 grupo hispida inclui T. alida sp. nov., T. hispida sp. nov. e T. periculosa sp. nov.; (6 grupo malefica inclui T. cactina sp. nov., T. curvata sp. nov., T. grallator sp. nov., T. jocosa sp. nov., T. mackameyi sp. nov., T. malefica sp. nov., T. obliqua (Walker e T. terencia sp. nov.; (7 grupo obtusa inclui T. obtusa Fowler, T. obunca sp. nov. e T. torosa sp. nov.; (8 grupo opponens inclui T. alvira sp. nov., T. arcuata sp. nov., T. damia sp. nov., T. insolita sp. nov., T. lunata sp. nov., T. lurida sp. nov., T. opponens

  11. A new species of Klabonosa Bouček (Hymenoptera: Pteromalidae) reared from eggs of Endochus sp. (Hemiptera: Reduviidae) from India.

    Science.gov (United States)

    Gupta, Ankita; Yeshwanth, H M; Sureshan, P M

    2018-04-23

    The genus Klabonosa Bouček (Hymenoptera: Pteromalidae) is recorded for the first time from the Oriental region, with both sexes of K. indica Gupta, Sureshan Yeshwanth sp. n. reared from eggs of the assassin bug Endochus sp. (Hemiptera: Reduviidae) on Artocarpus heterophyllus Lam. (Moraceae). The male is formally described and illustrated for the first time for the genus.

  12. Use of the entomopathogenic fungi Metarhizium anisopliae, Cordyceps bassiana and Isaria fumosorosea to control Diaphorina citri (Hemiptera: Psylidae) in Persian lime under field conditions

    Science.gov (United States)

    The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is a destructive insect pest in the citriculture, because it is an efficient vector of the proteobacteria, ‘Candidatus Liberibacter asiaticus’ (Las), ‘Ca. L. Africanus’ (Laf), and ‘Ca. L. Americanus’ (Lam). These bacteria c...

  13. Bioactivity of indigenous medicinal plants against the cotton whitefly, Bemisia tabaci.

    Science.gov (United States)

    Hammad, E Abou-Fakhr; Zeaiter, A; Saliba, N; Talhouk, S

    2014-01-01

    Forty-one methanol extracts of 28 indigenous medicinal plant species were tested for their insecticidal bioactivity against cotton whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), adults and second nymphal instars under controlled conditions. This study is within a bioprospection context, in the form of utilizing local plant species as an alternative in sustainable agriculture development. Eighteen and nine plant extracts caused a significant decrease in number of live adult and nymphal whiteflies, respectively, compared to the control. This is the first report for the potential effect on survival of insects for 22 out of 28 tested medicinal plant species. Whole plant extracts of Ranunculus myosuroudes Boiss. and Kotschy (Ranunculaceae), Achillea damascena L. (Asteraceae), and Anthemis hebronica Boiss. and Kotschy (Asteraceae) and leaf extracts of Verbascum leptostychum DC. (Scrophulariaceae) and Heliotropium rotundifolium Boiss. (Borangiaceae) caused both repellent and toxic effects against the adult and second nymphal instars, respectively. Extracts of leaves and stems of Anthemis scariosa Boiss. (Asteraceae) and Calendula palestina Pers. (Asteraceae) were found to be more bioactive against the adult and nymphal instars, respectively, than extracts of other plant parts, such as flowers. Thus, the bioactive extracts of these medicinal plants have the potential to lower whitefly populations in a comprehensive pest management program in local communities, pending cultivation of these medicinal plant species.

  14. Two cytochrome P450 genes are involved in imidacloprid resistance in field populations of the whitefly, Bemisia tabaci, in China.

    Science.gov (United States)

    Yang, Xin; Xie, Wen; Wang, Shao-li; Wu, Qing-jun; Pan, Hui-peng; Li, Ru-mei; Yang, Ni-na; Liu, Bai-ming; Xu, Bao-yun; Zhou, Xiaomao; Zhang, You-jun

    2013-11-01

    The sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera:Aleyrodidae), is an invasive and damaging pest of field crops worldwide. The neonicotinoid insecticide imidacloprid has been widely used to control this pest. We assessed the species composition (B vs. Q), imidacloprid resistance, and association between imidacloprid resistance and the expression of five P450 genes for 14-17 B. tabaci populations in 12 provinces in China. Fifteen of 17 populations contained only B. tabaci Q, and two populations contained both B and Q. Seven of 17 populations exhibited moderate to high resistance to imidacloprid, and eight populations exhibited low resistance to imidacloprid, compared with the most susceptible field WHHB population. In a study of 14 of the populations, resistance level was correlated with the expression of the P450 genes CYP6CM1 and CYP4C64 but not with the expression of CYP6CX1, CYP6CX4, or CYP6DZ7. This study indicates that B. tabaci Q has a wider distribution in China than previously reported. Resistance to imidacloprid in field populations of B. tabaci is associated with the increased expression of two cytochrome P450 genes (CYP6CM1 and CYP4C64). Crown Copyright © 2013. Published by Elsevier Inc. All rights reserved.

  15. Occurrence of Entomopathogenic Fungi from Agricultural and Natural Ecosystems in Saltillo, México, and their Virulence Towards Thrips and Whiteflies

    Science.gov (United States)

    Sánchez-Peña, Sergio R.; Lara, Jorge San-Juan; Medina, Raúl F.

    2011-01-01

    Entomopathogenic fungi were collected from soil in four adjacent habitats (oak forest, agricultural soil, pine reforestation and chaparral habitat) in Saltillo, México using the insect bait method with Tenebrio molitor (L.) (Coleoptera: Tenebrionidae) larvae as bait. Overall, of the larvae exposed to soil, 171 (20%) hosted Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae), 25 (3%) hosted Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) and 1 (0.1%) hosted lsaria (=Paecilomyces) sp. (Hypocreales: Cordycipitaceae). B. bassiana was significantly more frequent on larvae exposed to oak forest soil. M. anisopliae was significantly more frequent on larvae exposed to agricultural soil. From the infected bait insects, 93 isolates of B. bassiana and 24 isolates of M. anisopliae were obtained. Strains were tested for their infectivity against Cuban laurel thrips, Gynaikothrips uzeli Zimmerman (Thysanoptera: Phlaeothripidae) and the greenhouse whitefly, Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae). B. bassiana isolates caused the highest mortality on thrips (some causing 88% mortality after 6 days); both fungal species caused similarly high mortality levels against whiteflies (75%) after 6 days. Large amounts of germplasm of entomopathogenic fungi, fundamentally B. bassiana and M. anisopliae, exist in the habitats sampled; pathogenicity varied among strains, and some strains possessed significant virulence. Soils in these habitats are reservoirs of diverse strains with potential for use in biocontrol. PMID:21521145

  16. A new species in the indigenous New Zealand soft scale insect genus Kalasiris Henderson & Hodgson (Hemiptera: Sternorrhyncha: Coccomorpha: Coccidae) on Gahnia setifolia (Cyperaceae).

    Science.gov (United States)

    Hodgson, Chris J; Gunawardana, D N; Richmond, J E

    2016-03-14

    The genus Kalasiris Henderson & Hodgson (Hemiptera: Sternorrhyncha: Coccomorpha: Coccidae) is currently only known from New Zealand. The adult female and pupa of a new species, K. martini Hodgson & Richmond are described and illustrated below and the possible taxonomic relationships of the genus to other New Zealand genera are discussed.

  17. Potential use of the fungus Beauveria bassiana against the western flower thrips Frankliniella occidentalis without reducing the effectiveness of its natural predator Orius sauteri (Hemiptera: Anthocoridae)

    Science.gov (United States)

    Orius sauteri (Poppius) (Hemiptera: Anthocoridae) is an important predator of western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae). Orius sauteri would be directly exposed to the entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin in the field should the fu...

  18. [Occurrence of Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae) in the state of Maranhão, Brazil].

    Science.gov (United States)

    de Lemos, Raimunda N S; da Silva, Gilson S; Araújo, José R G; Das Chagas, Evandro F; Moreira, Aldenise A; Soares, Ana T M

    2006-01-01

    Aleurocanthus woglumi Ashby in citrus and mango crops in the state of Maranhão, Brazil, is recorded. Samples of 2003 and 2004 from several counties were identified and deposited in the Insect Collection of the Laboratório de Entomologia of the Núcleo de Biotecnologia Agronômica, Universidade Estadual do Maranhão.

  19. Assessment of Feeding Acceptance and Injury of Kerman Pistachios, Pistacia vera, by Brown Marmorated Stink Bug (Hemiptera: Pentatomidae)

    OpenAIRE

    Lara, Jesús R; Kamiyama, Matthew T; Hernandez, Gabriel; Nay, Justin; Hoddle, Mark S

    2017-01-01

    Abstract In the United States, California (CA) is the primary commercial producer of pistachio nuts, Pistacia vera L. (Anacardiaceae). The brown marmorated stink bug (BMSB), Halyomorpha halys (Stål) (Hemiptera: Pentatomidae), an invasive and polyphagous insect pest from Asia, has established in urban areas in several pistachio-growing counties in CA. Breeding BMSB populations have not been detected in commercial pistachio acreage. However, the detection of BMSB in Kern and Fresno counties, ma...

  20. Nano-formulation enhances insecticidal activity of natural pyrethrins against Aphis gossypii (Hemiptera: Aphididae) and retains their harmless effect to non-target predators.

    Science.gov (United States)

    Papanikolaou, Nikos E; Kalaitzaki, Argyro; Karamaouna, Filitsa; Michaelakis, Antonios; Papadimitriou, Vassiliki; Dourtoglou, Vassilis; Papachristos, Dimitrios P

    2018-04-01

    The insecticidal activity of a new nano-formulated natural pyrethrin was examined on the cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), and the predators Coccinella septempunctata L. (Coleoptera: Coccinellidae) and Macrolophus pygmaeus Rambur (Hemiptera: Miridae), in respect with the nano-scale potential to create more effective and environmentally responsible pesticides. Pyrethrin was nano-formulated in two water-in-oil micro-emulsions based on safe biocompatible materials, i.e., lemon oil terpenes as dispersant, polysorbates as stabilizers, and mixtures of water with glycerol as the dispersed aqueous phase. Laboratory bioassays showed a superior insecticidal effect of the pyrethrin micro-emulsions compared to two commercial suspension concentrates of natural pyrethrins against the aphid. The nano-formulated pyrethrins were harmless, in terms of caused mortality and survival time, to L3 larvae and four-instar nymphs of the predators C. septempunctata and M. pygmaeus, respectively. We expect that these results can contribute to the application of nano-technology in optimization of pesticide formulation, with further opportunities in the development of effective plant protection products compatible with integrated pest management practices.

  1. Mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking Hibiscus rosa-sinensis L. in Malaysia, with two new country records

    Science.gov (United States)

    Sartiami, Dewi; Watson, Gillian W.; Mohamad Roff, M. N.; Idris, A. B.

    2016-11-01

    A survey of mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) attacking the national flower of Malaysia, Hibiscus rosa-sisnensis L. and Hibiscus spp. (Malvaceae) was conducted in Selangor and Kuala Lumpur, Malaysia, from January to March 2016. Adult females were mounted on microscope slides in Canada balsam. The five species identified were Ferrisia dasylirii (Cockerell), Maconellicoccus hirsutus (Green), Paracoccus marginatus Williams and Granara de Willink, Phenacoccus solenopsis (Tinsley) and Pseudococcus jackbeardsleyi Gimpel & Miller. Two of these, the invasive species Ferrisia dasylirii and P. solenopsis were introduced and first recorded in Malaysia.

  2. Mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) with oral rim ducts; description of a new genus and species from Turkey, and discussion of their higher classification within the Pseudococcidae.

    Science.gov (United States)

    Kaydan, Mehmet Bora; Szita, Éva

    2017-02-03

    A new monotypic mealybug genus with oral rim ducts, Bromusicoccus Kaydan gen. n. (Hemiptera: Pseudococcidae: Pseudococcinae), is described from Turkey. The higher classification of mealybug genera with oral rim tubular ducts worldwide is discussed and a key is provided to separate them.

  3. <em>Anthocoris nemorumem> (Heteroptera: Anthocoridae) as predator of cabbage pests - voracity and prey preference

    DEFF Research Database (Denmark)

    Simonsen, Marie-Louise Rugholm; Enkegaard, Annie; Bang, Camilla Nordborg

    2010-01-01

    Laboratory experiments were performed with adult female Anthocoris nemorum (Linnaeus) (Heteroptera: Anthocoridae) at 20°C ± 1°C, L16:D8, 60–70% RH to determine voracity and preference on cabbage aphids (Brevicoryne brassicae L.) (Hemiptera: Aphididae), diamondback moth larvae (Plutella xylostella L....... The results showed that A. nemorum is a voracious predator of B. brassicae, P. xylostella and F. occidentalis and can therefore be considered as a potential candidate for biological control in cabbage....

  4. Reference Gene Selection in the Desert Plant <em>Eremosparton songoricuem>m>

    Directory of Open Access Journals (Sweden)

    Dao-Yuan Zhang

    2012-06-01

    Full Text Available <em>Eremosparton songoricum em>(Litv. Vass. (<em>E. songoricumem> is a rare and extremely drought-tolerant desert plant that holds promise as a model organism for the identification of genes associated with water deficit stress. Here, we cloned and evaluated the expression of eight candidate reference genes using quantitative real-time reverse transcriptase polymerase chain reactions. The expression of these candidate reference genes was analyzed in a diverse set of 20 samples including various <em>E. songoricumem> plant tissues exposed to multiple environmental stresses. GeNorm analysis indicated that expression stability varied between the reference genes in the different experimental conditions, but the two most stable reference genes were sufficient for normalization in most conditions.<em> EsEFem> and <em>Esα-TUB> were sufficient for various stress conditions, <em>EsEF> and <em>EsACT> were suitable for samples of differing germination stages, and <em>EsGAPDH>and <em>Es>UBQ em>were most stable across multiple adult tissue samples. The <em>Es18Sem> gene was unsuitable as a reference gene in our analysis. In addition, the expression level of the drought-stress related transcription factor <em>EsDREB2em>> em>verified the utility of<em> E. songoricumem> reference genes and indicated that no single gene was adequate for normalization on its own. This is the first systematic report on the selection of reference genes in <em>E. songoricumem>, and these data will facilitate future work on gene expression in this species.

  5. Monitoramento populacional das cigarrinhas vetoras de Xylella fastidiosa, através de armadilhas adesivas amarelas em pomares comerciais de citros Population monitoring of leafhopper vectors of Xylella fastidiosa, through yellow sticky traps in commercial citrus orchards

    Directory of Open Access Journals (Sweden)

    Rúbia de Oliveira Molina

    2010-12-01

    Full Text Available A Clorose variegada dos citros (CVC é uma doença causada pela bactéria de xilema Xylella fastidiosa Wells. A disseminação ocorre por meio de insetos vetores pertencente à ordem Hemiptera, família Cicadellidae (subfamília Cicadellinae, os quais transmitem a bactéria depois de se alimentarem em plantas contaminadas. Neste trabalho, objetivou-se identificar e monitorar as espécies de cigarrinhas vetoras em um pomar comercial no município de Paranavaí, Paraná. O experimento foi realizado em um talhão comercial de laranja doce (Citrus sinensis (L. Osbeck, variedade Pera, com 1.000 plantas de dez anos de idade. A amostragem foi realizada por meio de armadilhas adesivas amarelas, distribuídas na área periférica e central do pomar, com duas repetições por rua amostrada. As etiquetas foram distribuídas entre a 5ª e 30ª plantas em 10 ruas, totalizando 20 armadilhas que foram renovadas no pomar, a cada trinta dias, durante o período de avaliação que foi entre junho de 2005 e setembro de 2006. As principais espécies de Cicadellinae capturadas foram Acrogonia citrina Marucci & Cavichioli, Dilobopterus costalimai Young e Macugonalia cavifrons Stal. Essas espécies apresentaram ocorrência constante e frequência de 3,97%, 4,2%, 13,0% respectivamente, em relação ao total de cigarrinhas coletadas.Citrus variegated chlorosis (CVC is the disease caused by the bacteria Xylella fastidiosa Wells. Dissemination occurs through insect vectors belonging to the order Hemiptera, family Cicadellidae (subfamily Cicadellinae, which transmit the bacteria after feeding on infected plants. The objective of this study was to identify the species of insect vectors in an orchard in the municipality of Paranavaí, in the State of Paraná. The experiment was conducted in a commercial stand of sweet orange (Citrus sinensis (L. Osbeck, Pêra variety with 1,000 10-year-old plants. Monitoring was performed using yellow sticky traps, distributed in the central

  6. Stylet biogenesis in Bactericera cockerelli (Hemiptera: Triozidae).

    Science.gov (United States)

    Cicero, Joseph M

    2017-07-01

    The discovery of 'Ca. Liberibacter solanacearum', causal agent of certain solanaceous and apiaceous crop diseases, inside the functional (intrastadial) and pharate stylet anatomy of the potato psyllid prompted elucidation of the mechanism of stylet replacement as a novel exit portal in the transmission pathway. In Hemiptera, presumptive (formative) stylets, secreted during consecutive pharate instars, replace functional stylets lost with the exuviae. In potato psyllids, each functional stylet has a hollow core filled with a cytology that extends out of the core to form a hemispherical aggregate of cells, the 'end-cap', somewhat resembling a golf ball on a tee. A tightly folded mass of extremely thin cells, the 'matrix', occurs inside the end-cap. Micrograph interpretations indicate that during the pharate stage, the end-cap apolyses from the core and 'deconstructs' to release and expand the matrix into a long, coiled tube, the 'atrium'. Cells that were in contact with the inner walls of the functional stylet core maintain their position at the apex of the tube, and secrete a new stylet, apex first, the growing length of which descends into the tube until completed. They then despool from the coils into their functional position as the exuviae is shed. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Description of a new species of Hemiberlesia Cockerell (Hemiptera: Coccomorpha: Diaspididae) from China, with a key to Hemiberlesia species known from China.

    Science.gov (United States)

    Liu, Di; Feng, Jinian

    2018-03-06

    The diagnostic characters of the genus Hemiberlesia Cockerell (Hemiptera: Coccoidea: Diaspididae) are reviewed. The adult female, adult male and all immature stages (except the male prepupa and pupa) of a new species, H. serrulata Liu Feng sp. n., collected from Henan Province, China, are described and illustrated. A key is provided to identify the Hemiberlesia species known to occur in China.

  8. Identification of defensive compounds in metathoracic glands of adults of the stink bug Dichelops melacanthus (Hemiptera: Pentatomidae)

    Energy Technology Data Exchange (ETDEWEB)

    Marques, Francisco A.; Wendler, Edison P.; Maia, Beatriz Helena L.N. Sales [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Dept. de Quimica]. E-mail: francisco.marques@pesquisador.cnpq.br; Ventura, Mauricio U.; Arruda-Gatti, Iara Cintra [Universidade Estadual de Londrina (UEL), PR (Brazil). Centro de Ciencias Agrarias. Dept. de Agronomia

    2007-07-01

    The contents of metathoracic glands of adults of the stink bug Dichelops melacanthus (Hemiptera: Pentatomidae) were analyzed. Compounds were identified by gas chromatography (GC), coupled GC-mass spectrometry and matching retention indices and mass spectra with those of authentic samples. Tridecane was the major component followed by lesser and approximately equal amounts of (E)-4-oxo-2-hexenal and (E)-2-octenal. Other compounds identified include (E)-2-hexenal, decane, (E)-2-hexenyl acetate, undecane, (E)-4-oxo-2-octenal, dodecane, (E)-2-octenyl acetate, 1-tridecene, tetradecane and pentadecane. (author)

  9. Identification of defensive compounds in metathoracic glands of adults of the stink bug Dichelops melacanthus (Hemiptera: Pentatomidae)

    International Nuclear Information System (INIS)

    Marques, Francisco A.; Wendler, Edison P.; Maia, Beatriz Helena L.N. Sales; Ventura, Mauricio U.; Arruda-Gatti, Iara Cintra

    2007-01-01

    The contents of metathoracic glands of adults of the stink bug Dichelops melacanthus (Hemiptera: Pentatomidae) were analyzed. Compounds were identified by gas chromatography (GC), coupled GC-mass spectrometry and matching retention indices and mass spectra with those of authentic samples. Tridecane was the major component followed by lesser and approximately equal amounts of (E)-4-oxo-2-hexenal and (E)-2-octenal. Other compounds identified include (E)-2-hexenal, decane, (E)-2-hexenyl acetate, undecane, (E)-4-oxo-2-octenal, dodecane, (E)-2-octenyl acetate, 1-tridecene, tetradecane and pentadecane. (author)

  10. A specimen of <em>Sorex> cfr. <em>samniticus> in Barn Owl's pellets from Murge plateau (Apulia, Italy / Su di un <em>Sorex> cfr. <em>samniticus> (Insectivora, Soricidae rinvenuto in borre di <em>Tyto albaem> delle Murge (Puglia, Italia

    Directory of Open Access Journals (Sweden)

    Giovanni Ferrara

    1992-07-01

    Full Text Available Abstract In a lot of Barn Owl's pellets from the Murge plateau a specimen of <em>Sorex> sp. was detected. Thank to some morphological and morphometrical features, the cranial bones can be tentatively attributed to <em>Sorex samniticusem> Altobello, 1926. The genus <em>Sorex> was not yet included in the Apulia's fauna southwards of the Gargano district; the origin and significance of the above record is briefly discussed, the actual presence of a natural population of <em>Sorex> in the Murge being not yet proved. Riassunto Viene segnalato il rinvenimento di un esemplare di <em>Sorex> cfr. <em>samniticus> da borre di <em>Tyto albaem> delle Murge. Poiché il genere non era stato ancora segnalato nella Puglia a sud del Gargano, viene discusso il significato faunistico del reperto.

  11. Efecto de extractos vegetales de <em>Polygonum hydropiperoidesem>, <em>Solanum nigrumem> y <em>Calliandra pittieriem> sobre el gusano cogollero (<em>Spodoptera frugiperdaem>

    Directory of Open Access Journals (Sweden)

    Lizarazo H. Karol

    2008-12-01

    Full Text Available

    El gusano cogollero <em>Spodoptera frugiperdaem> es una de las plagas que más afectan los cultivos en la región de Sumapaz (Cundinamarca, Colombia. En la actualidad se controla principalmente aplicando productos de síntesis química, sin embargo la aplicación de extractos vegetales surge como una alternativa de menor impacto sobre el ambiente. Este control se emplea debido a que las plantas contienen metabolitos secundarios que pueden inhibir el desarrollo de los insectos. Por tal motivo, la presente investigación evaluó el efecto insecticida y antialimentario de extractos vegetales de barbasco <em>Polygonum hydropiperoidesem> (Polygonaceae, carbonero <em>Calliandra pittieriem> (Mimosaceae y hierba mora <em>Solanum nigrumem> (Solanaceae sobre larvas de <em>S. frugiperdaem> biotipo maíz. Se estableció una cría masiva del insecto en el laboratorio utilizando una dieta natural con hojas de maíz. Posteriormente se obtuvieron extractos vegetales utilizando solventes de alta polaridad (agua y etanol y media polaridad (diclorometano los cuales se aplicaron sobre las larvas de segundo instar. Los resultados más destacados se presentaron con extractos de <em>P. hydropiperoidesem>, obtenidos con diclorometano en sus diferentes dosis, con los cuales se alcanzó una mortalidad de 100% 12 días después de la aplicación y un efecto antialimentario representado por un consumo de follaje de maíz inferior al 4%, efectos similares a los del testigo comercial (Clorpiriphos.

  12. First occurrence of Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae) preying on defoliating caterpillars of oil palm in the state of Para, Brazil; Primeira ocorrencia de Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae) predando lagartas desfolhadoras do dendezeiro no estado do Para, Brasil

    Energy Technology Data Exchange (ETDEWEB)

    Ribeiro, Rafael C.; Lemos, Walkymario P.; Muller, Antonio A. [EMBRAPA Amazonia Oriental, Belem, PA (Brazil). Lab. de Entomologia], e-mail: rafaufra@yahoo.com.br, e-mail: wplemos@cpatu.embrapa.br; Muller, Antonio A. [Embrapa Amazonia Oriental, Belem, PA (Brazil). Lab. de Entomologia; Bernardino, Aline S.; Buecke, Joel [Grupo Agropalma S/A., Tailandia, PA (Brazil)

    2010-01-15

    The oil palm Elaeis guineensis is usually attacked by pests, particularly, defoliating caterpillars. Between 2004 and 2006 a stinkbug predator (Asopinae) was registered preying on caterpillars of Brassolis sophorae L., Opsiphanes invirae Hubner (Lepidoptera: Nymphalidae) and Sibine spp. (Lepidoptera: Limacodidae), reducing their populations in commercial oil palm plantations in the State of Para, Brazil. Specimens of the natural enemy were collected, mounted, and identified as Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae), corresponding to the first report of the occurrence of this stinkbug attacking defoliating caterpillars of oil palm in Brazil. (author)

  13. Rapid Development of Microsatellite Markers with 454 Pyrosequencing in a Vulnerable Fish<em>,> the Mottled Skate<em>, Raja em>pulchra>

    Directory of Open Access Journals (Sweden)

    Jung-Ha Kang

    2012-06-01

    Full Text Available The mottled skate, <em>Raja pulchraem>, is an economically valuable fish. However, due to a severe population decline, it is listed as a vulnerable species by the International Union for Conservation of Nature. To analyze its genetic structure and diversity, microsatellite markers were developed using 454 pyrosequencing. A total of 17,033 reads containing dinucleotide microsatellite repeat units (mean, 487 base pairs were identified from 453,549 reads. Among 32 loci containing more than nine repeat units, 20 primer sets (62% produced strong PCR products, of which 14 were polymorphic. In an analysis of 60 individuals from two <em>R. pulchra em>populations, the number of alleles per locus ranged from 1–10, and the mean allelic richness was 4.7. No linkage disequilibrium was found between any pair of loci, indicating that the markers were independent. The Hardy–Weinberg equilibrium test showed significant deviation in two of the 28 single-loci after sequential Bonferroni’s correction. Using 11 primer sets, cross-species amplification was demonstrated in nine related species from four families within two classes. Among the 11 loci amplified from three other <em>Rajidae> family species; three loci were polymorphic. A monomorphic locus was amplified in all three <em>Rajidae> family species and the <em>Dasyatidae> family. Two <em>Rajidae> polymorphic loci amplified monomorphic target DNAs in four species belonging to the Carcharhiniformes class, and another was polymorphic in two Carcharhiniformes species.

  14. First Record of Diaphorina citri (Hemiptera: Psyllidae) in Ecuador Infesting Urban Citrus and Orange Jasmine Trees

    Science.gov (United States)

    Cornejo, J.F.; Chica, E.J.

    2014-01-01

    Abstract Adults and nymphs of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), were collected in the Guayaquil, Samborondón, and Durán cantons in coastal Ecuador. Psyllids were found in high numbers in citrus ( Citrus spp., Sapindales: Rutaceae) and orange jasmine ( Murraya exotica [L.] Jack, Sapindales: Rutaceae) trees within the Guayaquil-Samborondon-Duran conurbation; however, none was found during scoutings in the main citrus producing areas in coastal Ecuador. To the best of our knowledge, this is the first report of D. citri in Ecuador and the Pacific coastal plain of South America. PMID:25527601

  15. Data on the scale insect (Hemiptera: Coccomorpha) fauna of Greece, with description of two new species.

    Science.gov (United States)

    Szita, Éva; Fetykó, Kinga Gabriela; Benedicty, Zsuzsanna Konczné; Kozár, Ferenc; Partsinevelos, Georgios; Milonas, Panagiotis; Kaydan, Mehmet Bora

    2017-10-09

    Surveys of the scale insect (Hemiptera: Coccomorpha) fauna of Greece were carried out in 2013 and 2014. Altogether 93 scale insect species were collected, belonging to 11 families. Thirty-eight species (41%) proved to be new to the Greek fauna, including two species new to science (Anophococcus hellenicus Kaydan & Szita sp. n. (Acanthococcidae) and Iberococcus attikus Szita & Fetykó sp. n. (Pseudococcidae)), and two introduced invasive species (Phenacoccus graminicola Leonardi and Pseudococcus comstocki (Kuwana), both Pseudococcidae). The rest of the species seem to be native to the Greek fauna. The total number of scale insect species recorded from Greece is increased to 253.

  16. Grupos de insetos visitantes florais da lichieira (Litchi chinensis Sonn. em Jaboticabal, estado de São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Ivan Carlos Fernandes Martins

    2013-01-01

    Full Text Available Os objetivos deste estudo foram identificar grupos de insetos visitantes de flores da lichieira (Litchi chinensis Sonn., determinar horários de maior visitação, analisar a influência da altura das inflorescências na ocorrência desses visitantes florais e verificar se a incidência de sol beneficia sua presença. O experimento foi desenvolvido na Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista FCAV/UNESP. As observações ocorreram em agosto e setembro de 2008, totalizando cinco em todo o período. Foram estabelecidos dois períodos diários de observação das 10:00 às 12:00 h e 16:00 às 18:00 h, sendo observado quatro pontos na área. Os dados dos visitantes florais observados para as diferentes horas, alturas e radiação solar foram submetidos à análise de variância e as médias foram comparadas pelo teste de Tukey 5%. Foram observadas seis ordens de insetos: Hymenoptera, Diptera, Hemiptera, Coleoptera, Lepidoptera e Neuroptera. O horário das 10:00 às 12:00 horas apresentou- se como mais propício para observação de insetos visitantes. Houve diferença nas observações das alturas de 0 a 0.5 e 1.6 m e 2.0 e também uma diferença nas observações de insetos visitantes florais da lichieira quando estas se encontravam em face sombra ou face sol na árvore.

  17. Densidades y frecuencias de liberación de Encarsia formosa (Hymenoptera: Aphelinidae sobre Trialeurodes vaporariorum (Homoptera: Aleyrodidae en tomate

    Directory of Open Access Journals (Sweden)

    Tello Paola

    2007-12-01

    Full Text Available

    La mosca blanca de los invernaderos, Trialeurodes vaporariorum (Westwood (Homoptera: Aleyrodidae es una de las plagas más importantes de cultivos de tomate bajo invernadero en la Sabana de Bogotá. Para su control tradicionalmente se acude a la aplicación de insecticidas. Sin embargo, también son contempladas liberaciones del parasitoide Encarsia formosa (Gahan (Hymenoptera: Aphelinidae. Para estandarizar el proceso de cría masiva de E. formosa, se evaluaron tres densidades diferentes de liberación de las avispas (6, 12 y 18 avispas/planta en tres frecuencias de liberación (1, 2 y 3 veces/semana, sobre plantas de tomate de ocho semanas de edad con tres niveles de infestación (15, 30 y 45 ninfas de mosca blanca/hoja, susceptibles de ser parasitadas. El mayor porcentaje de parasitismo (70% en ninfas de tercer instar se logró cuando se liberaron seis avispas por planta, dosificadas en tres momentos diferentes en la misma semana. El menor porcentaje de parasitismo (50%, se obtuvo cuando se realizó una sola liberación de 6, 12 o 18 avispas. Los resultados anteriores permiten concluir que se puede lograr un porcentaje de parasitismo en torno a un 70% de la infestación de mosca blanca (hasta 45 ninfas/hoja en plantas de tomate con pocas avispas de E. formosa (seis cuando se liberan en tres momentos diferentes.

  18. Especies de miridae (hemiptera) registradas en algunos cultivos tropicales en Chanchamayo y Satipo. Junín - Perú

    OpenAIRE

    Arellano, Germán; Vergara, Clorinda

    2016-01-01

    El presente estudio tuvo como objetivo identificar las especies de insectos Miridae(Hemiptera) presentes en seis cultivos tropicales en la selva del Departamento de Junín, Perú. Se considera como base las recolecciones realizadas entre los años 1983 a 1987. El muestreo se basó en colectas periódicas semanales de insectos en las plantaciones de cafeto (Coffea arabica L.), papayo (Carica papaya L.), piña (Ananas comosus var. comosus (L.) Merr., Coppens & Leal), palto (Persea americana Mill), cí...

  19. A New Natural Lactone from <em>Dimocarpus> <em>longan> Lour. Seeds

    Directory of Open Access Journals (Sweden)

    Zhongjun Li

    2012-08-01

    Full Text Available A new natural product named longanlactone was isolated from <em>Dimocarpus> <em>longan> Lour. seeds. Its structure was determined as 3-(2-acetyl-1<em>H>-pyrrol-1-yl-5-(prop-2-yn-1-yldihydrofuran-2(3H-one by spectroscopic methods and HRESIMS.

  20. Purification, Characterization and Antioxidant Activities <em>in Vitroem>> em>and <em>in Vivoem> of the Polysaccharides from <em>Boletus edulisem> Bull

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    Yijun Fan

    2012-07-01

    Full Text Available A water-soluble polysaccharide (BEBP was extracted from <em>Boletus edulis em>Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and BEBP-3. In the next experiment, the average molecular weight (Mw, IR and monosaccharide compositional analysis of the three polysaccharide fractions were determined. The evaluation of antioxidant activities both <em>in vitroem> and <em>in vivo em>suggested that BEBP-3 had good potential antioxidant activity, and should be explored as a novel potential antioxidant.

  1. <em>Angiostrongylus vasorumem> in red foxes (<em>Vulpes vulpesem> and badgers (<em>Meles melesem> from Central and Northern Italy

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    Marta Magi

    2010-06-01

    Full Text Available Abstract During 2004-2005 and 2007-2008, 189 foxes (<em>Vulpes vulpesem> and 6 badgers (<em>Meles melesem> were collected in different areas of Central Northern Italy (Piedmont, Liguria and Tuscany and examined for <em>Angiostrongylus vasorumem> infection. The prevalence of the infection was significantly different in the areas considered, with the highest values in the district of Imperia (80%, Liguria and in Montezemolo (70%, southern Piedmont; the prevalence in Tuscany was 7%. One badger collected in the area of Imperia turned out to be infected, representing the first report of the parasite in this species in Italy. Further studies are needed to evaluate the role played by fox populations as reservoirs of infection and the probability of its spreading to domestic dogs.
    Riassunto <em>Angiostrongylus vasorumem> nella volpe (<em>Vulpes vulpesem> e nel tasso (<em>Meles melesem> in Italia centro-settentrionale. Nel 2004-2005 e 2007-2008, 189 volpi (<em>Vulpes vulpesem> e 6 tassi (<em>Meles melesem> provenienti da differenti aree dell'Italia settentrionale e centrale (Piemonte, Liguria Toscana, sono stati esaminati per la ricerca di <em>Angiostrongylus vasorumem>. La prevalenza del nematode è risultata significativamente diversa nelle varie zone, con valori elevati nelle zone di Imperia (80% e di Montezemolo (70%, provincia di Cuneo; la prevalenza in Toscana è risultata del 7%. Un tasso proveniente dall'area di Imperia è risultato positivo per A. vasorum; questa è la prima segnalazione del parassita in tale specie in Italia. Ulteriori studi sono necessari per valutare il potenziale della volpe come serbatoio e la possibilità di diffusione della parassitosi ai cani domestici.

    doi:10.4404/hystrix-20.2-4442

  2. DNA Barcodes for Nearctic Auchenorrhyncha (Insecta: Hemiptera)

    Science.gov (United States)

    Foottit, Robert G.; Maw, Eric; Hebert, P. D. N.

    2014-01-01

    Background Many studies have shown the suitability of sequence variation in the 5′ region of the mitochondrial cytochrome c oxidase I (COI) gene as a DNA barcode for the identification of species in a wide range of animal groups. We examined 471 species in 147 genera of Hemiptera: Auchenorrhyncha drawn from specimens in the Canadian National Collection of Insects to assess the effectiveness of DNA barcoding in this group. Methodology/Principal Findings Analysis of the COI gene revealed less than 2% intra-specific divergence in 93% of the taxa examined, while minimum interspecific distances exceeded 2% in 70% of congeneric species pairs. Although most species are characterized by a distinct sequence cluster, sequences for members of many groups of closely related species either shared sequences or showed close similarity, with 25% of species separated from their nearest neighbor by less than 1%. Conclusions/Significance This study, although preliminary, provides DNA barcodes for about 8% of the species of this hemipteran suborder found in North America north of Mexico. Barcodes can enable the identification of many species of Auchenorrhyncha, but members of some species groups cannot be discriminated. Future use of DNA barcodes in regulatory, pest management, and environmental applications will be possible as the barcode library for Auchenorrhyncha expands to include more species and broader geographic coverage. PMID:25004106

  3. Assessment of Genetic Fidelity in <em>Rauvolfia em>s>erpentina em>Plantlets Grown from Synthetic (Encapsulated Seeds Following <em>in Vitroem> Storage at 4 °C

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    Mohammad Anis

    2012-05-01

    Full Text Available An efficient method was developed for plant regeneration and establishment from alginate encapsulated synthetic seeds of <em>Rauvolfia serpentinaem>. Synthetic seeds were produced using <em>in vitroem> proliferated microshoots upon complexation of 3% sodium alginate prepared in Llyod and McCown woody plant medium (WPM and 100 mM calcium chloride. Re-growth ability of encapsulated nodal segments was evaluated after storage at 4 °C for 0, 1, 2, 4, 6 and 8 weeks and compared with non-encapsulated buds. Effects of different media <em>viz>; Murashige and Skoog medium; Lloyd and McCown woody Plant medium, Gamborg’s B5 medium and Schenk and Hildebrandt medium was also investigated for conversion into plantlets. The maximum frequency of conversion into plantlets from encapsulated nodal segments stored at 4 °C for 4 weeks was achieved on woody plant medium supplement with 5.0 μM BA and 1.0 μM NAA. Rooting in plantlets was achieved in half-strength Murashige and Skoog liquid medium containing 0.5 μM indole-3-acetic acid (IAA on filter paper bridges. Plantlets obtained from stored synseeds were hardened, established successfully <em>ex vitroem> and were morphologically similar to each other as well as their mother plant. The genetic fidelity of <em>Rauvolfia em>clones raised from synthetic seeds following four weeks of storage at 4 °C were assessed by using random amplified polymorphic<em> em>DNA (RAPD and inter-simple sequence repeat<em> em>(ISSR markers. All the RAPD and ISSR profiles from generated plantlets were monomorphic and comparable<em> em>to the mother plant, which confirms the genetic<em> em>stability among the clones. This synseed protocol could be useful for establishing a particular system for conservation, short-term storage and production of genetically identical and stable plants before it is released for commercial purposes.

  4. Sulla presenza di <em>Sorex antinoriiem>, <em>Neomys anomalusem> (Insectivora, Soricidae e <em>Talpa caecaem> (Insectivora, Talpidae in Umbria

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    A.M. Paci

    2003-10-01

    Full Text Available Lo scopo del contributo è di fornire un aggiornamento sulla presenza del Toporagno del Vallese <em>Sorex antinoriiem>, del Toporagno acquatico di Miller <em>Neomys anomalusem> e della Talpa cieca <em>Talpa caecaem> in Umbria, dove le specie risultano accertate ormai da qualche anno. A tal fine sono stati rivisitati i reperti collezionati e la bibliografia conosciuta. Toporagno del Vallese: elevato di recente a livello di specie da Brünner et al. (2002, altrimenti considerato sottospecie del Toporagno comune (<em>S. araneus antinoriiem>. È conservato uno di tre crani incompleti (mancano mandibole ed incisivi superiori al momento prudenzialmente riferiti a <em>Sorex> cfr. <em>antinorii>, provenienti dall?Appennino umbro-marchigiano settentrionale (dintorni di Scalocchio - PG, 590 m. s.l.m. e determinati sulla base della pigmentazione rossa degli ipoconi del M1 e M2; Toporagno acquatico di Miller: tre crani (Breda in Paci e Romano op. cit. e un esemplare intero (Paci, ined. sono stati trovati a pochi chilometri di distanza gli uni dall?altro tra i comuni di Assisi e Valfabbrica, in ambienti mediocollinari limitrofi al Parco Regionale del M.te Subasio (Perugia. In provincia di Terni la specie viene segnalata da Isotti (op. cit. per i dintorni di Orvieto. Talpa cieca: sono noti una femmina e un maschio raccolti nel comune di Pietralunga (PG, rispettivamente in una conifereta a <em>Pinus nigraem> (m. 630 s.l.m. e nelle vicinanze di un bosco misto collinare a prevalenza di <em>Quercus cerrisem> (m. 640 s.l.m.. Recentemente un terzo individuo è stato rinvenuto nel comune di Sigillo (PG, all?interno del Parco Regionale di M.te Cucco, sul margine di una faggeta a 1100 m s.l.m. In entrambi i casi l?areale della specie è risultato parapatrico con quello di <em>Talpa europaeaem>.

  5. Morphology of the mouthparts of the spittlebug Philagra albinotata Uhler (Hemiptera: Cercopoidea: Aphrophoridae).

    Science.gov (United States)

    Wang, Tingting; Pan, Liuxing; Zhang, Yalin; Dai, Wu

    2015-03-01

    Mouthparts associated with feeding behavior and feeding habits are important sensory and feeding structures in insects. To obtain a better understanding of feeding in Cercopoidea, the morphology of mouthparts of the spittlebug, Philagra albinotata Uhler was examined using scanning electron microscopy. The mouthparts of P. albinotata are of the typical piercing-sucking type found in Hemiptera, comprising a cone-shaped labrum, a tube-like, three-segmented labium with a deep groove on the anterior side, and a stylet fascicle consisting of two mandibular and two maxillary stylets. The mandibles consist of a dorsal smooth region and a ventral serrate region near the apical half of the external convex region, and bear five nodules or teeth on the dorsal external convex region on the distal extremity; these are regarded as unique features that distinguish spittlebugs from other groups of Hemiptera. The externally smooth maxillary stylets, interlocked to form a larger food canal and a smaller salivary canal, are asymmetrical only in the internal position of longitudinal carinae and grooves. One dendritic canal is found in each maxilla and one in each mandible. Two types of sensilla trichodea, three types of sensilla basiconica and groups of multi-peg structures occur in different locations on the labium, specifically the labial tip with two lateral lobes divided into anterior sensory fields with ten small peg sensilla arranged in a 5+4+1 pattern and one big peg sensillum, and posterior sensory fields with four sensilla trichodea. Compared with those of previously studied Auchenorrhyncha, the mouthparts of P. albinotata may be distinguished by the shape of the mandibles, the multi-peg structures and a tooth between the salivary canal and the food canal on the extreme end of the stylets. The mouthpart morphology is illustrated using scanning electron micrographs, and the taxonomic and putative functional significance of the different structures is briefly discussed

  6. Four Novel Cellulose Synthase (CESA Genes from <em>Birch> (<em>Betula platyphylla em>Suk. Involved in Primary and Secondary Cell Wall Biosynthesis

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    Xuemei Liu

    2012-09-01

    Full Text Available Cellulose synthase (CESA, which is an essential catalyst for the generation of plant cell wall biomass, is mainly encoded by the <em>CesA> gene family that contains ten or more members. In this study; four full-length cDNAs encoding CESA were isolated from<em> Betula platyphyllaem> Suk., which is an important timber species, using RT-PCR combined with the RACE method and were named as <em>BplCesA3em>, <em>−4em>,> −7 em>and> −8em>. These deduced CESAs contained the same typical domains and regions as their <em>Arabidopsis> homologs. The cDNA lengths differed among these four genes, as did the locations of the various protein domains inferred from the deduced amino acid sequences, which shared amino acid sequence identities ranging from only 63.8% to 70.5%. Real-time RT-PCR showed that all four <em>BplCesAs> were expressed at different levels in diverse tissues. Results indicated that BplCESA8 might be involved in secondary cell wall biosynthesis and floral development. BplCESA3 appeared in a unique expression pattern and was possibly involved in primary cell wall biosynthesis and seed development; it might also be related to the homogalacturonan synthesis. BplCESA7 and BplCESA4 may be related to the formation of a cellulose synthase complex and participate mainly in secondary cell wall biosynthesis. The extremely low expression abundance of the four BplCESAs in mature pollen suggested very little involvement of them in mature pollen formation in <em>Betula>. The distinct expression pattern of the four <em>BplCesAs> suggested they might participate in developments of various tissues and that they are possibly controlled by distinct mechanisms in <em>Betula.>

  7. Identification of unique volatile compounds associated with repelling whiteflies (Hemiptera: Aleyrodidae) in desert watermelon (Citrullus colocynthis)

    Science.gov (United States)

    Commercial watermelon cultivars (Citrullus lanatus var. lanatus) share a narrow genetic base and are susceptible to many insect pests and diseases. The insects include whiteflies which cause serious economic damages to this important cucurbit crop. However, several United States Plant Introduction a...

  8. Planthopper (Hemiptera: Flatidae) Parasitized by Larval Erythraeid Mite (Trombidiformes: Erythraeidae)—A Description of Two New Species From Western Madagascar

    Science.gov (United States)

    Mąkol, Joanna; Moniuszko, Hanna; Świerczewski, Dariusz; Stroiński, Adam

    2014-01-01

    Abstract Descriptions of Dambullaeus adonis Mąkol et Moniuszko sp. nov. (Trombidiformes: Erythraeidae, Callidosomatinae) and Latois nigrolineata Świerczewski et Stroiński sp. nov. (Hemiptera: Fulgoromorpha, Flatidae) from Madagascar are provided. The first host record for ectoparasitic larvae of Dambullaeus Haitlinger, 2001 and the first evidence on host–parasite association between flatid adult and erythraeid larvae are given. Genus Dambullaeus , known exclusively from larvae and now comprising two species of Gondwanan distribution, is critically reappraised. PMID:25434029

  9. Microsatellite Loci in the Gypsophyte <em>Lepidium subulatum em>(Brassicaceae, and Transferability to Other <em>Lepidieae>

    Directory of Open Access Journals (Sweden)

    José Gabriel Segarra-Moragues

    2012-09-01

    Full Text Available Polymorphic microsatellite markers were developed for the Ibero-North African, strict gypsophyte <em>Lepidium subulatumem> to unravel the effects of habitat fragmentation in levels of genetic diversity, genetic structure and gene flow among its populations. Using 454 pyrosequencing 12 microsatellite loci including di- and tri-nucleotide repeats were characterized in <em>L. subulatumem>. They amplified a total of 80 alleles (2–12 alleles per locus in a sample of 35 individuals of <em>L. subulatumem>, showing relatively high levels of genetic diversity, <em>H>O = 0.645, <em>H>E = 0.627. Cross-species transferability of all 12 loci was successful for the Iberian endemics <em>Lepidium cardaminesem>, <em>Lepidium stylatumem>, and the widespread, <em>Lepidium graminifoliumem> and one species each of two related genera, <em>Cardaria drabaem> and <em>Coronopus didymusem>. These microsatellite primers will be useful to investigate genetic diversity, population structure and to address conservation genetics in species of <em>Lepidium>.

  10. Toxicities of Selected Essential Oils, Silicone Oils, and Paraffin Oil against the Common Bed Bug (Hemiptera: Cimicidae).

    Science.gov (United States)

    Zha, Chen; Wang, Changlu; Li, Andrew

    2018-02-09

    The common bed bug [Cimex lectularius L. (Hemiptera: Cimicidae)] and tropical bed bug [Cimex hemipterus F. (Hemiptera: Cimicidae)] resurged in the United States and many other countries over the past decades. The need for safe and effective bed bug control products propelled the development of numerous 'green insecticides', mostly with essential oils listed as active ingredients. Various inorganic and organic oils also were used for bed bug management. However, there are no published studies on their toxicities against bed bugs. In this study, we screened 18 essential oils, three silicone oils, and paraffin oil (C5-20 paraffins) for their toxicities against bed bugs. All the oils exhibited insecticidal activity in topical assays. Their toxicities varied significantly; all of the evaluated essential oils were less effective than silicone oils and paraffin oil. The LD50 values of the most effective essential oil (blood orange), paraffin oil, and the most effective silicone oil (dodecamethylpentasiloxane) are 0.184 ± 0.018, 0.069 ± 0.012, and 0.036 ± 0.005 mg per bug, respectively. Direct spray of 1% water solution of 3-[hydroxy (polyethyleneoxy) propyl] heptamethyltrisiloxane, the only silicone oil that mixes well with water, resulted in 92% bed bug mortality after 1 d. Results of this study indicate silicone oils and paraffin oil have the potential to be used as safer alternative bed bug control materials. © The Author(s) 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  11. Espécies de cigarrinhas em cultivo de café no Município de Santa Teresa, Espírito Santo, Brasil (Hemiptera, Cicadellidae, Cicadellinae

    Directory of Open Access Journals (Sweden)

    Rachel A. Carvalho

    2015-01-01

    Full Text Available As cigarrinhas da subfamília Cicadellinae são importantes vetores de patógenos de plantas cultivadas, dentre eles, a bactéria Xylella fastidiosa, que, no Brasil, ataca cultivos de citros, café e também de ameixa. Pouca informação é conhecida sobre a ocorrência, distribuição e biologia de cicadelíneos em plantações de café. Este trabalho apresenta uma lista de 141 espécimes, coletados em meses alternados, no período entre junho de 2009 e abril de 2010, em plantação de café no Município de Santa Teresa no Estado do Espírito Santo, Brasil. Esses espécimes estão distribuídos em 16 gêneros e 21 espécies pertencentes às duas tribos de Cicadellinae: Cicadellini e Proconiini. Dentre os Cicadellini listados, o gênero Graphocephala é, pela primeira vez, registrado para o Brasil.

  12. Constituents from <em>Vigna em>vexillata> and Their Anti-Inflammatory Activity

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    Guo-Feng Chen

    2012-08-01

    Full Text Available The seeds of <em>Vigna em>genus are important food resources and there have already been many reports regarding their bioactivities. In our preliminary bioassay, the chloroform layer of methanol extracts of<em> V. vexillata em>demonstrated significant anti-inflammatory bioactivity. Therefore, the present research is aimed to purify and identify the anti-inflammatory principles of <em>V. vexillataem>. One new sterol (1 and two new isoflavones (2,3 were reported from the natural sources for the first time and their chemical structures were determined by the spectroscopic and mass spectrometric analyses. In addition, 37 known compounds were identified by comparison of their physical and spectroscopic data with those reported in the literature. Among the isolates, daidzein (23, abscisic acid (25, and quercetin (40 displayed the most significant inhibition of superoxide anion generation and elastase release.

  13. Synthesis, Crystal Structure and Luminescent Property of Cd (II Complex with <em>N-Benzenesulphonyl-L>-leucine

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    Xishi Tai

    2012-09-01

    Full Text Available A new trinuclear Cd (II complex [Cd3(L6(2,2-bipyridine3] [L =<em> Nem>-phenylsulfonyl-L>-leucinato] has been synthesized and characterized by elemental analysis, IR and X-ray single crystal diffraction analysis. The results show that the complex belongs to the orthorhombic, space group<em> Pem>212121 with<em> aem> = 16.877(3 Å, <em>b> em>= 22.875(5 Å, <em>c em>= 29.495(6 Å, <em>α> em>= <emem>= <emem>= 90°, <em>V> em>= 11387(4 Å3, <em>Z> em>= 4, <em>Dc>= 1.416 μg·m−3, <emem>= 0.737 mm−1, <em>F> em>(000 = 4992, and final <em>R>1 = 0.0390, <em>ωR>2 = 0.0989. The complex comprises two seven-coordinated Cd (II atoms, with a N2O5 distorted pengonal bipyramidal coordination environment and a six-coordinated Cd (II atom, with a N2O4 distorted octahedral coordination environment. The molecules form one dimensional chain structure by the interaction of bridged carboxylato groups, hydrogen bonds and p-p interaction of 2,2-bipyridine. The luminescent properties of the Cd (II complex and <em>N-Benzenesulphonyl-L>-leucine in solid and in CH3OH solution also have been investigated.

  14. A two-in-one superhydrophobic and anti-reflective nanodevice in the grey cicada Cicada orni (Hemiptera)

    Energy Technology Data Exchange (ETDEWEB)

    Dellieu, Louis, E-mail: louis.dellieu@unamur.be; Sarrazin, Michaël, E-mail: michael.sarrazin@unamur.be; Simonis, Priscilla; Deparis, Olivier; Vigneron, Jean Pol [Research Center in Physics of Matter and Radiation (PMR), Department of Physics, University of Namur (FUNDP), 61 rue de Bruxelles, B-5000 Namur (Belgium)

    2014-07-14

    Two separated levels of functionality are identified in the nanostructure which covers the wings of the grey cicada Cicada orni (Hemiptera). The upper level is responsible for superhydrophobic character of the wing, while the lower level enhances its anti-reflective behavior. Extensive wetting experiments with various chemical species and optical measurements were performed in order to assess the bi-functionality. Scanning electron microscopy imaging was used to identify the nanostructure morphology. Numerical optical simulations and analytical wetting models were used to prove the roles of both levels of the nanostructure. In addition, the complex refractive index of the chitinous material of the wing was determined from measurements.

  15. A two-in-one superhydrophobic and anti-reflective nanodevice in the grey cicada Cicada orni (Hemiptera)

    Science.gov (United States)

    Dellieu, Louis; Sarrazin, Michaël; Simonis, Priscilla; Deparis, Olivier; Vigneron, Jean Pol

    2014-07-01

    Two separated levels of functionality are identified in the nanostructure which covers the wings of the grey cicada Cicada orni (Hemiptera). The upper level is responsible for superhydrophobic character of the wing, while the lower level enhances its anti-reflective behavior. Extensive wetting experiments with various chemical species and optical measurements were performed in order to assess the bi-functionality. Scanning electron microscopy imaging was used to identify the nanostructure morphology. Numerical optical simulations and analytical wetting models were used to prove the roles of both levels of the nanostructure. In addition, the complex refractive index of the chitinous material of the wing was determined from measurements.

  16. A two-in-one superhydrophobic and anti-reflective nanodevice in the grey cicada Cicada orni (Hemiptera)

    International Nuclear Information System (INIS)

    Dellieu, Louis; Sarrazin, Michaël; Simonis, Priscilla; Deparis, Olivier; Vigneron, Jean Pol

    2014-01-01

    Two separated levels of functionality are identified in the nanostructure which covers the wings of the grey cicada Cicada orni (Hemiptera). The upper level is responsible for superhydrophobic character of the wing, while the lower level enhances its anti-reflective behavior. Extensive wetting experiments with various chemical species and optical measurements were performed in order to assess the bi-functionality. Scanning electron microscopy imaging was used to identify the nanostructure morphology. Numerical optical simulations and analytical wetting models were used to prove the roles of both levels of the nanostructure. In addition, the complex refractive index of the chitinous material of the wing was determined from measurements.

  17. Compatibilidad in vitro de Isaria fumosorosea (Wize Brown y Smith (Hypocreales: Clavicipitaceae con plaguicidas comerciales

    Directory of Open Access Journals (Sweden)

    Érika Paola Grijalba Bernal

    2014-01-01

    Full Text Available Los agricultores colombianos utilizan productos químicos con categorías toxicológicas I, II y III para el control de insectos como la mosca blanca Bemisia tabaci (Gennadius, 1889 (Hemiptera: Aleyrodidae; pero su uso indiscriminado genera un riesgo para la salud y el medio ambiente. El control microbiológico con hongos entomopatógenos surge como alternativa de control ambientalmente sostenible. No obstante, su empleo exitoso dentro de una estrategia de manejo integrado de plagas (MIP depende, en gran parte, del efecto de los productos químicos sobre el bioplaguicida. El objetivo del presente trabajo fue determinar la compatibilidad in vitro de un bioplaguicida con base en el hongo Isaria fumosorosea formulado como polvo mojable con cuatro fungicidas y cinco insecticidas comerciales. Para esto, se evaluó el efecto de tres concentraciones de los productos químicos: FR = dosis recomendada en campo, 0.5 x FR y 0.25 x FR sobre la germinación de los conidios y el número de unidades formadoras de colonia UFC/g de I. fumosorosea. Los cuatro fungicidas (Benlate, Carboxin Captan, Metalaxil-Mancozeb y Mancozeb en las tres dosis evaluadas fueron incompatibles con el bioplaguicida, mientras que el insecticida Thiametoxam fue compatible cuando se utilizó la dosis de 0.25 x FR.

  18. Polyol specificity of recombinant Arabidopsis thaliana sorbitol dehydrogenase studied by enzyme kinetics and in silico modeling

    Directory of Open Access Journals (Sweden)

    María Francisca eAguayo

    2015-02-01

    Full Text Available Polyols are enzymatically-produced plant compounds which can act as compatible solutes during periods of abiotic stress. NAD+-dependent SORBITOL DEHYDROGENASE (SDH, E.C. 1.1.1.14 from Arabidopsis thaliana L. (AtSDH is capable of oxidizing several polyols including sorbitol, ribitol and xylitol. In the present study, enzymatic assays using recombinant AtSDH demonstrated a higher specificity constant for xylitol compared to sorbitol and ribitol, all of which are C2 (S and C4 (R polyols. Enzyme activity was reduced by preincubation with ethylenediaminetetraacetic acid (EDTA, indicating a requirement for zinc ions. In humans, it has been proposed that sorbitol becomes part of a pentahedric coordination sphere of the catalytic zinc during the reaction mechanism. In order to determine the validity of this pentahedric coordination model in a plant SDH, homology modeling and Molecular Dynamics simulations of AtSDH ternary complexes with the three polyols were performed using crystal structures of human and Bemisia argentifolii (Genn. (Hemiptera: Aleyrodidae SDHs as scaffolds. The results indicate that the differences in interaction with structural water molecules correlate very well with the observed enzymatic parameters, validate the proposed pentahedric coordination of the catalytic zinc ion in a plant SDH, and provide an explanation for why AtSDH shows a preference for polyols with a chirality of C2 (S and C4 (R.

  19. Morphological characterization of Capsicum annuum L. accessions from southern Mexico and their response to the Bemisia tabaci-Begomovirus complex

    Directory of Open Access Journals (Sweden)

    Horacio Ballina-Gomez

    2013-12-01

    Full Text Available The high diversity of chili pepper (Capsicum annuum L. in Mexico offers an excellent alternative to search for wild and semi-domesticated genotypes as sources of resistance to the complex Bemisia tabaci (Genn. (Hemiptera: Aleyrodidae-Begomovirus, which has caused enormous losses in commercial production of various horticultural crops. The goal of the present work was to characterize ex situ 18 genotypes of C. annuum from southern Mexico through 47 morphological descriptors, and to evaluate its response to the B. tabaci-Begomovirus complex. Morphological characterization showed the variables calyx annular constriction (CAC, number of branch bifurcation (NBB, and calyx pigmentation (CP had the highest variation. Principal components analysis (PCA of 47 morphological characteristics showed that 12 components were selected as meaningful factors. These components explained 94% of the variation. Cluster analysis showed three major clusters and seven sub-clusters. On the other hand, evaluation of the response to B. tabaci-Begomovirus showed that the genotypes have differential susceptibility to this vector-pathogen complex. Genotypes 'Chawa', 'Blanco', 'Maax' and 'X'catic' were into the low susceptibility to B. tabaci and low severity of viral symptoms. Surprisingly, the genotype 'Simojovel' showed high susceptibility to whitefly, but was grouped into genotypes with low symptom severity. This study shows the potential of native germplasm of pepper to explore sources of resistance to the B. tabaci-Begomovirus complex.

  20. Population Structure of the Greenhouse Whitefly, Trialeurodes vaporariorum (Westwood, an Invasive Species from the Americas, 60 Years after Invading China

    Directory of Open Access Journals (Sweden)

    Rui-Rui Gao

    2014-08-01

    Full Text Available Though the greenhouse whitefly, Trialeurodes vaporariorum (Westwood (Hemiptera: Aleyrodidae was introduced into China more than 60 years ago, the genetic diversity and structure of this exotic insect pest and virus vector have not been studied. To investigate the population genetic characteristics of this invasive species and to identify potential invasion routes, the genetic diversity and population structure of 17 collections of T. vaporariorum from nine provinces in China were analyzed using seven microsatellite loci. The results of the analyses indicated that the genetic diversity for the populations examined from the four provinces: Jilin, Ningxia, Guizhou and Qinghai, was lower than the genetic diversity of populations from the five provinces: Yunnan, Shandong, Shanxi, Liaoning, and Gansu. The T. vaporariorum populations analyzed in this study grouped as two distinct genetic clusters based on the analysis using STRUCTURE, whereas, 8 clusters were identified based on the BAPS analysis. Of the 136 genetic distance (Fst values, 128 (94% were associated with a significant exact test. However, there was no significant relationship between Fst and geographical distance. These results demonstrate that populations of T. vaporariorum in China exhibit significant genetic differentiation, indicating the likelihood that multiple introductions of T. vaporariorum into China have occurred. Also, the populations collected from the provinces of Jilin, Ningxia, Guizhou and Qinghai appear to represent secondary introductions originating from other Chinese provinces.

  1. Electrostatic Insect Sweeper for Eliminating Whiteflies Colonizing Host Plants: A Complementary Pest Control Device in An Electric Field Screen-Guarded Greenhouse.

    Science.gov (United States)

    Takikawa, Yoshihiro; Matsuda, Yoshinori; Kakutani, Koji; Nonomura, Teruo; Kusakari, Shin-Ichi; Okada, Kiyotsugu; Kimbara, Junji; Osamura, Kazumi; Toyoda, Hideyoshi

    2015-05-12

    Our greenhouse tomatoes have suffered from attacks by viruliferous whiteflies Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) over the last 10 years. The fundamental countermeasure was the application of an electric field screen to the greenhouse windows to prevent their entry. However, while the protection was effective, it was incomplete, because of the lack of a guard at the greenhouse entrance area; in fact, the pests entered from the entrance door when workers entered and exited. To address this, we developed a portable electrostatic insect sweeper as a supplementary technique to the screen. In this sweeper, eight insulated conductor wires (ICWs) were arranged at constant intervals along a polyvinylchloride (PVC) pipe and covered with a cylindrical stainless net. The ICWs and metal net were linked to a DC voltage generator (operated by 3-V alkaline batteries) inside the grip and oppositely electrified to generate an electric field between them. Whiteflies on the plants were attracted to the sweeper that was gently slid along the leaves. This apparatus was easy to operate on-site in a greenhouse and enabled capture of the whiteflies detected during the routine care of the tomato plants. Using this apparatus, we caught all whiteflies that invaded the non-guarded entrance door and minimized the appearance and spread of the viral disease in tomato plants in the greenhouse.

  2. Bioactivity of indigenous medicinal plants against the cotton whitefly, Bemisia tabaci

    Science.gov (United States)

    Hammad, E. Abou-Fakhr; Zeaiter, A.; Saliba, N.; Talhouk, S.

    2014-01-01

    Abstract Forty-one methanol extracts of 28 indigenous medicinal plant species were tested for their insecticidal bioactivity against cotton whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), adults and second nymphal instars under controlled conditions. This study is within a bioprospection context, in the form of utilizing local plant species as an alternative in sustainable agriculture development. Eighteen and nine plant extracts caused a significant decrease in number of live adult and nymphal whiteflies, respectively, compared to the control. This is the first report for the potential effect on survival of insects for 22 out of 28 tested medicinal plant species. Whole plant extracts of Ranunculus myosuroudes Boiss. and Kotschy (Ranunculaceae), Achillea damascena L. (Asteraceae), and Anthemis hebronica Boiss. and Kotschy (Asteraceae) and leaf extracts of Verbascum leptostychum DC. (Scrophulariaceae) and Heliotropium rotundifolium Boiss. (Borangiaceae) caused both repellent and toxic effects against the adult and second nymphal instars, respectively. Extracts of leaves and stems of Anthemis scariosa Boiss. (Asteraceae) and Calendula palestina Pers. (Asteraceae) were found to be more bioactive against the adult and nymphal instars, respectively, than extracts of other plant parts, such as flowers. Thus, the bioactive extracts of these medicinal plants have the potential to lower whitefly populations in a comprehensive pest management program in local communities, pending cultivation of these medicinal plant species. PMID:25204756

  3. <em>N>-Substituted 5-Chloro-6-phenylpyridazin-3(2<em>H>-ones: Synthesis, Insecticidal Activity Against <em>Plutella xylostella em>(L. and SAR Study

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    Song Yang

    2012-08-01

    Full Text Available A series of <em>N>-substituted 5-chloro-6-phenylpyridazin-3(2<em>H>-one derivatives were synthesized based on our previous work; all compounds were characterized by spectral data and tested for <em>in vitroem> insecticidal activity against <em>Plutella xylostellaem>. The results showed that the synthesized pyridazin-3(2<em>H>-one compounds possessed good insecticidal activities, especially the compounds 4b, 4d, and 4h which showed > 90% activity at 100 mg/L. The structure-activity relationships (SAR for these compounds were also discussed.

  4. Primer registro de Conidiobolus coronatus (Zygomycetes: Entomophthorales en crías experimentales de dos especies plaga del maíz: Delphacodes kuscheli y D. haywardi (Hemiptera: Delphacidae en la Argentina First record of Conidiobolus coronatus (Zygomycetes: Entomophthorales in experimental breeding of two pest species of corn: Delphacodes kuscheli and D. haywardi Muir (Hemiptera: Delphacidae in Argentine

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    A. V. Toledo

    Full Text Available Se investigó la ocurrencia natural del hongo entomopatógeno Conidiobolus coronatus (Costantin Batko (Zygomycetes: Entomophthorales en adultos de Delphacodes kuscheli Fennah y D. haywardi Muir (Hemiptera: Delphacidae, criados sobre Hordeum vulgare L. bajo condiciones de invernadero. Los insectos muertos, por una sospechada infección fúngica, fueron recolectados, esterilizados superficialmente, y examinados en el laboratorio. Conidiobolus coronatus fue aislado en cultivos puros, descrito morfológicamente y depositado en colecciones micológicas. Este trabajo presenta el primer registro de C. coronatus contra insectos perjudiciales en la Argentina.The natural occurrence of the entomopathogenic fungus Conidiobolus coronatus (Costantin Batko (Zygomycetes: Entomophthorales in adults of Delphacodes kuscheli Fennah and D. haywardi Muir (Hemiptera: Delphacidae, reared on Hordeum vulgare L. under greenhouse conditions, was investigated. Dead insects, suspected of fungal infection, were collected, surface sterilized, and examined in the laboratory. Conidiobolus coronatus was isolated in pure cultures, described morphologically, and deposited in mycological collections. This paper presents the first record of C. coronatus against harmful insects in Argentina.

  5. Phytophagous insects of giant hogweed <em>Heracleum mantegazzianumem> (Apiaceae) in invaded areas of Europe and in its native area of the Caucasus

    DEFF Research Database (Denmark)

    Hansen, Steen Ole; Hattendorf, Jan; Wittenberg, Ruediger

    2006-01-01

    Giant hogweed, Heracleum mantegazzianum (Apiaceae), was introduced from the Caucasus into Western Europe more than 150 years ago and later became all invasive weed which created major problems for European authorities. Phytophagous insects were collected in the native range of the giant hogweed (...... herbivores were restricted to a few taxonomic groups, especially within the Hemiptera, and were particularly abundant oil this weed....

  6. Organization of the mitochondrial genomes of whiteflies, aphids, and psyllids (Hemiptera, Sternorrhyncha

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    Baumann Paul

    2004-08-01

    Full Text Available Abstract Background With some exceptions, mitochondria within the class Insecta have the same gene content, and generally, a similar gene order allowing the proposal of an ancestral gene order. The principal exceptions are several orders within the Hemipteroid assemblage including the order Thysanoptera, a sister group of the order Hemiptera. Within the Hemiptera, there are available a number of completely sequenced mitochondrial genomes that have a gene order similar to that of the proposed ancestor. None, however, are available from the suborder Sternorryncha that includes whiteflies, psyllids and aphids. Results We have determined the complete nucleotide sequence of the mitochondrial genomes of six species of whiteflies, one psyllid and one aphid. Two species of whiteflies, one psyllid and one aphid have mitochondrial genomes with a gene order very similar to that of the proposed insect ancestor. The remaining four species of whiteflies had variations in the gene order. In all cases, there was the excision of a DNA fragment encoding for cytochrome oxidase subunit III(COIII-tRNAgly-NADH dehydrogenase subunit 3(ND3-tRNAala-tRNAarg-tRNAasn from the ancestral position between genes for ATP synthase subunit 6 and NADH dehydrogenase subunit 5. Based on the position in which all or part of this fragment was inserted, the mitochondria could be subdivided into four different gene arrangement types. PCR amplification spanning from COIII to genes outside the inserted region and sequence determination of the resulting fragments, indicated that different whitefly species could be placed into one of these arrangement types. A phylogenetic analysis of 19 whitefly species based on genes for mitochondrial cytochrome b, NADH dehydrogenase subunit 1, and 16S ribosomal DNA as well as cospeciating endosymbiont 16S and 23S ribosomal DNA indicated a clustering of species that corresponded to the gene arrangement types. Conclusions In whiteflies, the region of the

  7. Are Phenacoccus solani Ferris and P. defectus Ferris (Hemiptera: Pseudococcidae) distinct species?

    Science.gov (United States)

    Chatzidimitriou, Evangelia; Simonato, Mauro; Watson, Gillian W; Martinez-Sañudo, Isabel; Tanaka, Hirotaka; Zhao, Jing; Pellizzari, Giuseppina

    2016-03-24

    Among the Nearctic species of Phenacoccus (Hemiptera: Pseudococcidae), Phenacoccus solani Ferris and P. defectus Ferris are morphologically similar and it can be difficult to separate them on the basis of microscopic morphological characters of the adult female alone. In order to resolve their identity, a canonical variates morphological analysis of 199 specimens from different geographical origins and host plants and a molecular analysis of the COI and 28S genes were performed. The morphological analysis supported synonymy of the two species, as although the type specimens of the "species" are widely separated from each other in the canonical variates plot, they are all part of a continuous range of variation. The molecular analysis showed that P. solani and P. defectus are grouped in the same clade. On the basis of the morphological and molecular analyses, P. defectus is synonymized under the senior name P. solani, syn. n.

  8. New species of Rhinoleucophenga, a potential predator of pineapple mealybugs Nova espécie de Rhinoleucophenga, potencial predadora da cochonilha-do-abacaxizeiro

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    Mark Paul Culik

    2009-04-01

    Full Text Available The objective of this work was to describe a new species of Rhinoleucophenga (Diptera: Drosophilidae. Rhinoleucophenga capixabensis sp. nov. is described based on specimens collected from pineapple (Ananas comosus var. comosus infested with Dysmicoccus brevipes (Hemiptera: Pseudococcidae in the state of Espírito Santo, Brazil. Distinguishing characters of R. capixabensis sp. nov. include hyaline wings, a strong seta on the second antennal segment, body length of about 2-3 mm, uniformly dark brown scutellum, one pair of strong prescutellar setae, ventral epandrial lobes with about 17-18 teeth, and female cerci with approximately 20 long setae.O objetivo deste trabalho foi descrever uma nova espécie de Rhinoleucophenga (Diptera: Drosophilidae. Rhinoleucophenga capixabensis sp. nov. foi descrita com base em espécimes coletados em associação com Dysmicoccus brevipes (Hemiptera: Pseudococcidae em Ananas comosus var. comosus, no Estado do Espírito Santo, Brasil. As principais características de R. capixabensis sp. nov. incluem asas hialinas, uma seta forte no segundo segmento da antena, comprimento do corpo de aproximadamente 2-3 mm, escutelo uniformemente castanho-escuro, um par robusto de setas prescutelares, lobos epadriais ventrais com aproximadamente 17-18 dentes e cercos da fêmea com aproximadamente 20 setas longas.

  9. INFLUÊNCIA ESTOICA NA CONCEPÇÃO DE <em>STATUS> E <em>DICTUM> COMO <em> QUASI RES EM> (ὡσανεì τινά EM ABERLARDO STOIC INFLUENCE IN ABELARD'S CONCEPTION OF <em>STATUS> AND <em>DICTUM> AS <em>QUASI RESem> (ὡσανεì τινά.

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    Guy Hamelin

    2011-09-01

    Full Text Available Na sua obra, Pedro Abelardo (1079-1142 destaca duas noções metafísicas que fundamentam sua teoria lógica: o <em style="mso-bidi-font-style: normal;">statusem> e o <em style="mso-bidi-font-style: normal;">dictum propositionisem>, ao causar, respectivamente, a imposição (<em style="mso-bidi-font-style: normal;">impositioem> dos termos universais e o valor de verdade das proposições. Trata-se de expressões que se referem a naturezas ontológicas peculiares, na medida em que não são consideradas coisas (<em style="mso-bidi-font-style: normal;">resem>, mesmo que constituem causas. Todavia, também não são nada. Abelardo as chama de ‘quase coisas’ (<em style="mso-bidi-font-style: normal;">quasi resem>. No presente artigo, explicamos, primeiro, essas duas noções essenciais da lógica abelardiana, antes de tentar, em seguida, encontrar a fonte dessa metafísica particular. Em oposição a comentadores importantes da lógica de Abelardo, que estimam que haja uma forte influência platônica sobre essa concepção específica, defendemos antes, com apoio de textos significativos e de acordo com o nominalismo abelardiano, que a maior ascendência sobre a metafísica do nosso autor é a do estoicismo, sobretudo, antigo.In his work, Peter Abelard (1079-1142 highlights two metaphysical notions, which sustain his logical theory: the <em>status> and the <em>dictum propositionisem>, causing respectively both the imposition (<em>impositio> of universal terms and the thuth-value of propositions. Both expressions refer to peculiar ontological natures, in so far as they are not considered things (<em>res>, even if they constitute causes. Nevertheless, neither are they ‘nothing’. Abelard calls them ‘quasi-things’ (<em>quasi resem>. In the present article, we expound first these two essential notions of Abelardian logic before then trying to find the source of this particular metaphysics. Contrary to some important

  10. Evaluasi Sifat Ovisidal dan Nimfasidal Insektisida Buprofezin 100 g/l terhadap Telur dan Nimfa Wereng Coklat, Nilaparvata lugens (Stal.) (Hemiptera: Delphacidae)

    OpenAIRE

    BAEHAKI SUHERLAN EFFENDI; EKO HARI ISWANTO; AMIR HAMZAH

    2016-01-01

    Evaluation of ovicidal and nymphcidal properties of buprofezin 100 g/linsecticide to eggs and nymphs of brown planthopper, Nilaparvata lugens(Stal.) (Hemiptera: Delphacidae). Research was carried out in screen house ofSukamandi, Indonesian Center for Rice Research (ICRR) in the wet season of 2013. Theresearch used randomized block design with 5 doses levels insecticide treatment of buprofezin100 g/l and four replications. The buprofezin treatments were 250, 500, 750, 1000, and 1500ml/ha compa...

  11. Desempenho alimentar e sobrevivência de Euschistus heros parasitado por Hexacladia smithii em sementes de soja

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    Nunes Maria Clarice

    2002-01-01

    Full Text Available Avaliou-se, em laboratório, a influência do parasitóide Hexacladia smithii Ashmead (Hymenoptera: Encyrtidae na atividade alimentar e sobrevivência de Euschistus heros (Fabricius (Hemiptera: Pentatomidae, aos 10, 20, 30 e 40 dias após o início do parasitismo. A atividade alimentar foi avaliada pelo número de bainhas constatadas nas sementes de soja, Glycine max (L. Merrill (Fabaceae, em 48 horas de alimentação. O teste foi mantido sob condições controladas de temperatura (25±2°C, umidade (65±10% e fotoperíodo (14L:10E. No início do desenvolvimento de H. smithii a atividade alimentar dos percevejos não-parasitados e parasitados foi semelhante, obtendo-se, entretanto, duas vezes mais bainhas alimentares depositadas/grão pelos machos e fêmeas, após 30 dias de parasitismo. A sobrevivência dos percevejos parasitados foi menor que a dos percevejos não-parasitados, alcançando 100% de mortalidade 40 a 50 dias após o início do parasitismo. A redução na sobrevivência dos percevejos parasitados por H. smithii e a atividade alimentar, semelhante a dos percevejos não-parasitados durante quase todo o período de desenvolvimento do parasitóide, destacam a contribuição desse parasitismo na redução da população do percevejo-marrom E. heros.

  12. Spatial-temporal distribution of sharpshooters (Hemyptera: Cicadellidae insect vectors of Xylella fastidiosa in citrus orchards = Distribuição espaço-temporal de cigarrinhas (Hemiptera: Cicadellidae vetores da Xylella fastidiosa em pomares cítricos

    Directory of Open Access Journals (Sweden)

    Rúbia de Oliveira Molina

    2016-07-01

    Full Text Available Variegated chlorosis (CVC is a citrus disease, reported initially in the northwest of São Paulo state and in the Triângulo Mineiro region of Minas Gerais state in 1987. The CVC is caused by the xylematic bacteria Xylella fastidiosa. The bacteria is spread through contaminated bubbles or by insect vectors belonging to the Hemyptera order and Cicadellidae family. The aimed of this study was to identify the species of Xylella fastidiosa insect vector and to determine its spatial and temporal distribution in commercial orchards of sweet orange [Citrus sinensis (L. Osbeck]. The experiment was conducted in a commercial area of sweet orange, Pêra variety, grafted on Rangpur lime, located in northwest Paraná. For sampling, yellow sticky traps were used, distributed in the peripheral and central area of the orchard with four replicates per street sampled (5, 30, 55 and 80th plant, each plant was considered a sample unit. Were evaluated ten plots per street, totaling 40 traps for sampling. Every thirty days during the evaluation period, the traps were renewed in the orchard. The main species caught were Acrogonia citrine and Dilobopterus costalimai. The highest incidences occurred from winter to spring, and summer to autumn of the next year. According to the geostatistical analysis, the spatial distribution of these species concentrated in the peripheral zone of the portion where a higher incidence of these species was captured. The results show that it is necessary to adopt pest management practices for the Cicadellidae vector of X. fastidiosa differentiated in space and time. = A clorose variegada dos citros (CVC é uma doença de plantas cítricas, constatada, em 1987, inicialmente nos municípios do noroeste paulista e da região do triângulo mineiro. Ela é causada por uma bactéria de xilema, denominada Xylella fastidiosa. Sua disseminação ocorre através de borbulhas contaminadas ou por meio de insetos vetores da ordem Hemiptera e fam

  13. Systematics and phylogeny of the Neotropical treehopper subfamily Nicomiinae (Hemiptera, Membracidae Sistemática e filogenia das cigarrinhas neotropicais da subfamília Nicomiinae (Hemiptera, Membracidae

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    Jesse L. Albertson

    2005-03-01

    Full Text Available Morphological characters of adults of the treehopper subfamily Nicomiinae Haupt, 1929 (Hemiptera, Membracidae including seven genera (Eudonica gen. nov.; Euwalkeria Goding, 1926; Holdgatiella Evans, 1962; Nicomia Stål, 1858; Nodonica Dietrich, McKamey& Deitz, 2001; Stalomia gen. nov.; and Tolania Stål, 1858 and 22 species (16 new are described and illustrated. Keys are provided for genera and for species of Euwalkeria, Holdgatiella, and Nicomia. Nomenclatural changes, based on study of the primary type material of 15 species, include three new combinations, one new synonymy, and reinstatement of one junior synonym. Eudonica has one species, Eudonica nanella sp. nov.; Euwalkeria has five species, including four new species: E. colorata sp. nov., E. distincta sp. nov., E. perdita sp. nov., E. rubrica sp. nov.; Holdgatiella has two species, one of which is described as new: Holdgatiella chiloensis sp. nov.; Nicomia has twelve species, nine of which are described as new: N. buccina sp. nov., N. harenosa sp. nov., N. inscripta sp. nov., N. jucunda sp. nov., N. monticola sp. nov., N. nigrifasciata sp. nov., N. notidana sp. nov., N. pulchella sp. nov., N. serrata sp. nov.; Nodonica has one species, Nodonica bispinigera Dietrich, McKamey & Deitz; and Stalomia has one species, Stalomia veruta sp. nov. Tolania contains eleven previously described species and nearly 60 new species, which will be treated in a later publication. Three new combinations are proposed: one species described in Nicomia is placed in the tribe Abelini (Centrotinae as Abelus retrospinosus (Lethierry comb. nov.; one species previously placed in Nicomia is transferred to the genus Tolania as T. obliqua (Walker, 1858, comb. nov.; one species described in Holdgatiella is placed in the genus Tolania as T. stria (Cryan & Deitz, 2002, comb. nov. One new synonymy is proposed: Hoplophera [sic] cicadoides Walker, 1862, syn. nov., a junior synonym of Nicomia interrupta Stål, 1858. Nicomia

  14. Spatial distribution of Diaphorina citri Kuwayama (Hemiptera: Psyllidae in citrus orchards Distribuição espacial de Diaphorina citri Kuwayama (Hemiptera: Psyllidae em pomares de citros

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    Marilia Gregolin Costa

    2010-10-01

    Full Text Available The psyllid Diaphorina citri Kuwayama is one of the most important pests of citrus, mainly because it is the vector of the bacterium that causes huanglongbing (HLB or 'Greening' disease. To study the spatial distribution of nymphs and adults of this pest, an experiment was carried out in two 'Valencia' sweet orange orchards, four and 12 years of age, established in Matão, central area of São Paulo state, Brazil. The following dispersion indices were used to study pest aggregation in the citrus plants: variance/mean relationship (I, index of Morisita (Iδ, coefficient of Green (Cx, the exponent of negative binomial distribution, common k (c and Taylor's Power Law for each sampling. The negative binomial distribution was more representative of the spatial distribution of this psyllid, for both nymphs and adults. For most samplings, psyllid nymphs found in branches and adults caught in traps had an aggregated distribution.O psilídeo Diaphorina citri Kuwayama tornou-se nos últimos anos uma das mais importantes pragas na cultura de citros, principalmente pelos prejuízos causados às plantas por ser o transmissor da bactéria causadora da doença Huanglongbing (HLB ou 'Greening'. Com a finalidade de estudar a distribuição espacial de ninfas e adultos desta praga, instalaram-se experimentos em duas áreas de citros com histórico de ocorrência de HLB, no município de Matão (região central do Estado de São Paulo, em plantas de laranja 'Valência', com quatro e 12 anos de idade. Para estudo da agregação da população nas plantas, foram utilizados os seguintes índices de dispersão: razão variância/média (I, índice de Morisita (Iδ, coeficiente de Green (Cx e expoente da distribuição binomial negativa, k comum (c e lei da potência de Taylor para cada amostragem. A distribuição binomial negativa foi o modelo mais adequado para representar a distribuição espacial do psilídeo, tanto para ninfas como para adultos. Na maioria das

  15. Insects found in birds' nests from Argentina. Pseudoseisura lophotes Reichenbach, 1853 and Anumbius annumbi (Vieillot, 1817) (Aves: Furnariidae), hosts of Triatoma platensis Neiva, 1913 (Hemiptera: Reduviidae: Triatominae).

    Science.gov (United States)

    Paola, Turienzo

    2014-02-24

    The insect fauna of the nests of Pseudoseisura lophotes (Reichenbach, 1853) (Aves: Furnariidae) from Argentina was investigated. A total of 110 species (68 identified to species, 22 identified to genus, 20 identified to family) in 40 families of 10 orders of insects was found in these nests. Triatoma platensis Neiva, 1913 (Hemiptera: Reduviidae) was found again in nests of P. lophotes, corroborating after 73 years the first observations made by Mazza in 1936. The occurrence of the insects in nests of P. lophotes is compared with the previously known insect fauna in nests of A. annumbi, Furnarius rufus (Furnariidae), and Myiopsitta monachus (Psittacidae). The insect fauna in additional nests of Anumbius annumbi from the same and/or different localities is given, and used in comparisons. The first occurrence of Cuterebridae (Diptera) in birds' nests, their pupae as the overwintering stage, and the second simultaneous infestation by two species of Philornis (Diptera: Muscidae) on the same nestlings are presented. Other simultaneous infestations of different hematophagous arthropods (Hemiptera: Cimidae; Reduviidae: Triatominae, and Acari: Argasidae) are remarked and discussed.

  16. Natural Products from Antarctic Colonial Ascidians of the Genera <em>Aplidium> and <em>Synoicum>: Variability and Defensive Role

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    Conxita Avila

    2012-08-01

    Full Text Available Ascidians have developed multiple defensive strategies mostly related to physical, nutritional or chemical properties of the tunic. One of such is chemical defense based on secondary metabolites. We analyzed a series of colonial Antarctic ascidians from deep-water collections belonging to the genera <em>Aplidium> and <em>Synoicum> to evaluate the incidence of organic deterrents and their variability. The ether fractions from 15 samples including specimens of the species <em>A.> <em>falklandicum>, <em>A.> <em>fuegiense>, <em>A.> <em>meridianum>, <em>A.> <em>millari> and <em>S.> <em>adareanum> were subjected to feeding assays towards two relevant sympatric predators: the starfish <em>Odontaster> <em>validus>, and the amphipod <em>Cheirimedon> <em>femoratus>. All samples revealed repellency. Nonetheless, some colonies concentrated defensive chemicals in internal body-regions rather than in the tunic. Four ascidian-derived meroterpenoids, rossinones B and the three derivatives 2,3-epoxy-rossinone B, 3-epi-rossinone B, 5,6-epoxy-rossinone B, and the indole alkaloids meridianins A–G, along with other minoritary meridianin compounds were isolated from several samples. Some purified metabolites were tested in feeding assays exhibiting potent unpalatabilities, thus revealing their role in predation avoidance. Ascidian extracts and purified compound-fractions were further assessed in antibacterial tests against a marine Antarctic bacterium. Only the meridianins showed inhibition activity, demonstrating a multifunctional defensive role. According to their occurrence in nature and within our colonial specimens, the possible origin of both types of metabolites is discussed.

  17. A new Anagyrus (Hymenoptera: Encyrtidae) from Argentina, parasitoid of Hypogeococcus sp. (Hemiptera: Pseudococcidae) on Harrisia pomanensis (Cactaceae).

    Science.gov (United States)

    Triapitsyn, Serguei V; Aguirre, María B; Logarzo, Guillermo A

    2016-05-26

    A new species of Anagyrus Howard (Hymenoptera: Encyrtidae), A. lapachosus sp. n., is described from Salta Province of Argentina as a parasitoid of Hypogeococcus sp. (Hemiptera: Pseudococcidae) on Harrisia pomanensis cactus (Cactaceae). It is a candidate "new association" biological control agent for quarantine evaluation and possible following introduction to Puerto Rico (USA) against another Hypogeococcus sp., commonly called the Harrisia cactus mealybug and often misidentified as H. pungens Granara de Willink (according to our unpublished data the latter attacks only Amaranthaceae), which devastates or threatens the native cacti there and also in some other Caribbean islands (Triapitsyn, Aguirre et al. 2014; Carrera-Martínez et al. 2015).

  18. Fumigant Antifungal Activity of Myrtaceae Essential Oils and Constituents from <em>Leptospermum petersoniiem> against Three <em>Aspergillus> Species

    Directory of Open Access Journals (Sweden)

    Il-Kwon Park

    2012-09-01

    Full Text Available Commercial plant essential oils obtained from 11 Myrtaceae plant species were tested for their fumigant antifungal activity against <em>Aspergillus ochraceusem>, <em>A. flavusem>, and <em>A. nigerem>. Essential oils extracted from<em> em>Leptospermum> <em>petersonii> at air concentrations of 56 × 10−3 mg/mL and 28 × 10−3 mg/mL completely inhibited the growth of the three <em>Aspergillus> species. However, at an air concentration of 14 × 10−3 mg/mL, inhibition rates of <em>L. petersoniiem> essential oils were reduced to 20.2% and 18.8% in the case of <em>A. flavusem> and <em>A. nigerem>, respectively. The other Myrtaceae essential oils (56 × 10−3 mg/mL only weakly inhibited the fungi or had no detectable affect. Gas chromatography-mass spectrometry analysis identified 16 compounds in <em>L. petersoniiem>> em>essential> em>oil.> em>The antifungal activity of the identified compounds was tested individually by using standard or synthesized compounds. Of these, neral and geranial inhibited growth by 100%, at an air concentration of 56 × 10−3 mg/mL, whereas the activity of citronellol was somewhat lover (80%. The other compounds exhibited only moderate or weak antifungal activity. The antifungal activities of blends of constituents identified in <em>L. petersoniiem> oil indicated that neral and geranial were the major contributors to the fumigant and antifungal activities.

  19. Megamelus bellicus (Hemiptera: Delphacidae: immature stages and biology

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    Roxana MARIANI

    2007-01-01

    Full Text Available Se describen e ilustran los estados inmaduros de Megamelus bellicus Remes Lenicov & Sosa (Hemiptera: Delphacidae y se presenta una clave para identificarlos. La descripción de cada estadio se realizó sobre la base de ninfas extraidas 24 horas posteriores a la eclosión, de colonias de laboratorio y criadas sobre trozos de hojas de camalote Eichhornia crassipes (Martius Solms Laubach. Los principales caracteres para distinguir los distintos estadios son: tamaño del cuerpo, color, número de tarsómeros, espinulación de la metatibia y número de dientes del calcar y presencia de sensorios en el pedicelo antenal. Datos biológicos basados en observaciones en el laboratorio y en el campo, muestran que M. bellicus realiza su ciclo biológico exitosamente sobre Pontederiaceae. Los huevos, dispuestos de 1 a 9 por postura, son colocados profundamente en el aerénquima del pecíolo; son más frecuentes de 3-4, menos frecuentes 5, 2 y 6, y raramente 1, 7 y 9. Se registra el porcentaje de parasitoidismo de un Hymenoptera oófilo de la familia Eulophidae, Aprostocetus (Ootetrastichus sp. Debido a que M. bellicus ocupa el mismo hábitat ecológico que M. scutellaris Berg, se resaltan las principales diferencias morfológicas y de comportamiento entre las mismas.

  20. Clinical Relevance of <em>CDH1em> and <em>CDH13em> DNA-Methylation in Serum of Cervical Cancer Patients

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    Günther K. Bonn

    2012-07-01

    Full Text Available This study was designed to investigate the DNA-methylation status of <em>E>-cadherin (<em>CDH1em> and <em>H>-cadherin (<em>CDH13em> in serum samples of cervical cancer patients and control patients with no malignant diseases and to evaluate the clinical utility of these markers. DNA-methylation status of <em>CDH1em> and <em>CDH13em> was analyzed by means of MethyLight-technology in serum samples from 49 cervical cancer patients and 40 patients with diseases other than cancer. To compare this methylation analysis with another technique, we analyzed the samples with a denaturing high performance liquid chromatography (DHPLC PCR-method. The specificity and sensitivity of <em>CDH1em> DNA-methylation measured by MethyLight was 75% and 55%, and for <em>CDH13em> DNA-methylation 95% and 10%. We identified a specificity of 92.5% and a sensitivity of only 27% for the <em>CDH1em> DHPLC-PCR analysis. Multivariate analysis showed that serum <em>CDH1em> methylation-positive patients had a 7.8-fold risk for death (95% CI: 2.2–27.7; <em>p> = 0.001 and a 92.8-fold risk for relapse (95% CI: 3.9–2207.1; <em>p> = 0.005. We concluded that the serological detection of <em>CDH1em> and <em>CDH13em> DNA-hypermethylation is not an ideal diagnostic tool due to low diagnostic specificity and sensitivity. However, it was validated that <em>CDH1em> methylation analysis in serum samples may be of potential use as a prognostic marker for cervical cancer patients.

  1. Extraction of Dihydroquercetin<em> em>from <em>Larix gmeliniem>i> em>with Ultrasound-Assisted and Microwave-Assisted Alternant Digestion

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    Yuangang Zu

    2012-07-01

    Full Text Available An ultrasound and microwave assisted alternant extraction method (UMAE was applied for extracting dihydroquercetin (DHQ from <em>Larix gmeliniem>i> wood. This investigation was conducted using 60% ethanol as solvent, 1:12 solid to liquid ratio, and 3 h soaking time. The optimum treatment time was ultrasound 40 min, microwave 20 min, respectively, and the extraction was performed once. Under the optimized conditions, satisfactory extraction yield of the target analyte was obtained. Relative to ultrasound-assisted or microwave-assisted method, the proposed approach provides higher extraction yield. The effect of DHQ of different concentrations and synthetic antioxidants on oxidative stability in soy bean oil stored for 20 days at different temperatures (25 °C and 60 °C was compared. DHQ was more effective in restraining soy bean oil oxidation, and a dose-response relationship was observed. The antioxidant activity of DHQ was a little stronger than that of BHA and BHT. Soy bean oil supplemented with 0.08 mg/g DHQ exhibited favorable antioxidant effects and is preferable for effectively avoiding oxidation. The <em>L. gmeliniiem> wood samples before and after extraction were characterized by scanning electron microscopy. The results showed that the UMAE method is a simple and efficient technique for sample preparation.

  2. How <em>Varroa> Parasitism Affects the Immunological and Nutritional Status of the Honey Bee, <em>Apis melliferaem>

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    Katherine A. Aronstein

    2012-06-01

    Full Text Available We investigated the effect of the parasitic mite <em>Varroa destructorem> on the immunological and nutritional condition of honey bees, <em>Apis melliferaem>, from the perspective of the individual bee and the colony. Pupae, newly-emerged adults and foraging adults were sampled from honey bee colonies at one site in S. Texas, USA. <em>Varroa>‑infested bees displayed elevated titer of Deformed Wing Virus (DWV, suggestive of depressed capacity to limit viral replication. Expression of genes coding three anti-microbial peptides (<em>defensin1, abaecin, hymenoptaecinem> was either not significantly different between <em>Varroa>-infested and uninfested bees or was significantly elevated in <em>Varroa>-infested bees, varying with sampling date and bee developmental age. The effect of <em>Varroa> on nutritional indices of the bees was complex, with protein, triglyceride, glycogen and sugar levels strongly influenced by life-stage of the bee and individual colony. Protein content was depressed and free amino acid content elevated in <em>Varroa>-infested pupae, suggesting that protein synthesis, and consequently growth, may be limited in these insects. No simple relationship between the values of nutritional and immune-related indices was observed, and colony-scale effects were indicated by the reduced weight of pupae in colonies with high <em>Varroa> abundance, irrespective of whether the individual pupa bore <em>Varroa>.

  3. Trophic systems and chorology: data from shrews, moles and voles of Italy preyed by the barn owl / Sistemi trofici e corologia: dati su <em>Soricidae>, <em>Talpidae> ed <em>Arvicolidae> d'Italia predati da <em>Tyto albaem> (Scopoli 1769

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    Longino Contoli

    1986-12-01

    Full Text Available Abstract In small Mammals biogeography, available data are up to now by far too scanty for elucidate the distribution of a lot of taxa, especially with regard to the absence from a given area. In this respect, standardized quantitative sampling techniques, like Owl pellets analysis can enable not only to enhance faunistic knowledges, but also to estimate the actual absence probability of a given taxon "m", lacking from the diet of an individual raptor. For the last purpose, the relevant frequencies of "m" in the other ecologically similar sites of the same raptor species diets are averaged ($f_m$ : the relevant standard error (multiplicated by a coefficient, according to the desired degree of accuracy, in relation of the integral of probabilities subtracted ($overline{F}_m - a E$: then, the probability that a single specimen is not pertaining to "m" is obtained ($P_0 = 1 - F_m + a E$; lastly, the desiderate accuracy probability ($P_d$ is chosen. Now, "$N_d$" (the number of individuals of all prey species in a single site needed for obtain, with the desired probability, a specimen at least of "m" is obtained through $$N = frac{ln P_d}{ln P_0}$$ Obviously, every site-diet with more than "N" preyed individuals and without any "i" specimen is considered to be lacking of such taxon. A "usefulness index" for the above purposes is outlined and checked about three raptors. Some exanples about usefulness of the Owl pellet analysis method in biogeography are given, concerning <em>Tyto albaem> diets in peninsular Italy about: - <em>Sorex minutusem>, lacking in some quite insulated areas; - <em>Sorex araneusem> (sensu stricto, after GRAF et al., 1979, present also in lowland areas in Emilia-Romagna; - <em>Crocidura suaveolensem> and - <em>Suncus etruscusem>, present also in the southermost part of Calabria (Reggio province; - <em>Talpa caecaem>, present also in the Antiapennines of Latium (Cimini mounts; - <em>Talpa romanaem

  4. Spectral Detection of Soybean Aphid (Hemiptera: Aphididae) and Confounding Insecticide Effects in Soybean

    Science.gov (United States)

    Alves, Tavvs Micael

    Soybean aphid, Aphis glycines (Hemiptera: Aphididae) is the primary insect pest of soybean in the northcentral United States. Soybean aphid may cause stunted plants, leaf discoloration, plant death, and decrease soybean yield by 40%. Sampling plans have been developed for supporting soybean aphid management. However, growers' perception about time involved in direct insect counts has been contributing to a lower adoption of traditional pest scouting methods and may be associated with the use of prophylactic insecticide applications in soybean. Remote sensing of plant spectral (light-derived) responses to soybean aphid feeding is a promising alternative to estimate injury without direct insect counts and, thus, increase adoption and efficiency of scouting programs. This research explored the use of remote sensing of soybean reflectance for detection of soybean aphids and showed that foliar insecticides may have implications for subsequent use of soybean spectral reflectance for pest detection. (Abstract shortened by ProQuest.).

  5. Ocorrência de Quesada gigas em Schizolobium amazonicum em municípios do Maranhão e do Pará

    OpenAIRE

    Zanuncio, José Cola; Pereira, Fabrício Fagundes; Zanuncio, Teresinha Vinha; Martinelli, Nilza Maria; Pinon, Tobias Baruc Moreira; Guimarães, Edylene Marota

    2004-01-01

    An infestation of Quesada gigas Olivier (Hemiptera: Cicadidae) on "paricá" Schizolobium amazonicum (Huber) Ducken (Fabales: Fabaceae) was reported in the Municipalities of Itinga, Maranhão State and Paragominas, Pará State. Nymphs of this insect on roots and adults and exuvias on trunks of the plant were observed. Exit holes of nymphs in the soil were also observed. The occurrence of Q. gigas on S. amazonicum shows the damage potential of this species and the necessity of developing integrate...

  6. Response of Bemisia tabaci Genn. (Hemiptera: Aleyrodidae) biotype B to genotypes of pepper Capsicum annuum (Solanales: Solanaceae).

    Science.gov (United States)

    Ballina-Gomez, H; Ruiz-Sanchez, E; Chan-Cupul, W; Latournerie-Moreno, L; Hernández-Alvarado, L; Islas-Flores, I; Zuñiga-Aguilar, J J

    2013-04-01

    Bemisia tabaci Genn. biotype B is a widely distributed plant pest that represents one of the major constraints for horticultural crop production. The purpose of the present work was to evaluate the oviposition preference, survivorship, and development of B. tabaci biotype B on semi-cultivated genotypes of Capsicum annuum from southeast Mexico. In free-choice experiments to evaluate the oviposition preference, lower number of eggs laid by B. tabaci biotype B was observed in the genotypes Maax and Xcat´ik relative to that in the commercial genotype Parado. Egg hatchability was significantly lower in Pico Paloma, Bolita, Blanco, Chawa, Payaso, and Xcat´ik than in the rest of the genotypes, including the commercial genotype Jalapeño. Likewise, survivorship of nymphs was significantly lower in Pico Paloma, Bolita, and Blanco than in the remaining genotypes. Nymph developmental time and the period of development from egg to adult were the shortest in Amaxito. Therefore, sources of resistance to B. tabaci biotype B by antibiosis (accumulation of plant defense compounds) might be found in the semi-cultivated genotypes Pico Paloma, Bolita, and Blanco.

  7. Susceptibility of Bemisia tabaci MEAM1 (Hemiptera: Aleyrodidae) to imidacloprid, thiamethoxam, dinotefuran and flupyradifurone in south Florida

    Science.gov (United States)

    Populations of Bemisa tabaci Middle East Asia Minor 1 (MEAM 1) were established from nineteen locations in south Florida, primarily from commercial tomato fields, and were tested using a cotton leaf petiole systemic uptake method for susceptibility to the nicotinic acetylcholine agonist insecticides...

  8. Susceptibility of Bemisia tabaci MEAM1 (Hemiptera: Aleyrodidae to Imidacloprid, Thiamethoxam, Dinotefuran and Flupyradifurone in South Florida

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    Hugh A. Smith

    2016-10-01

    Full Text Available Populations of Bemisa tabaci MEAM1 were established from nineteen locations in south Florida, primarily from commercial tomato fields, and were tested using a cotton leaf petiole systemic uptake method for susceptibility to the nicotinic acetylcholine agonist insecticides imidacloprid, thiamethoxam, dinotefuran and flupyradifurone. Eleven populations produced LC50s for one or more chemicals that were not significantly different from the susceptible laboratory colony based on overlapping fiducial limits, indicating some degree of susceptibility. LC50s more than a 100-fold the laboratory colony were measured in at least one population for each material tested, indicating tolerance. LC50s (ppm from field populations ranged from 0.901–24.952 for imidacloprid, 0.965–24.430 for thiamethoxam, 0.043–3.350 for dinotefuran and 0.011–1.471 for flupyradifurone. Based on overlapping fiducial limits, there were no significant differences in relative mean potency estimates for flupyradifurone and dinotefuran in relation to imidacloprid and thiamethoxam.

  9. High Level of Nitrogen Makes Tomato Plants Releasing Less Volatiles and Attracting More Bemisia tabaci (Hemiptera: Aleyrodidae)

    Science.gov (United States)

    Islam, Md. Nazrul; Hasanuzzaman, Abu Tayeb Mohammad; Zhang, Zhan-Feng; Zhang, Yi; Liu, Tong-Xian

    2017-01-01

    Tomato (Solanum lycopersicum) production is seriously hampered by the infestation of the sweetpotato whitefly, Bemisia tabaci MEAM 1 (Middle East-Asia Minor 1). The infestation behavior of the whiteflies could be affected by the quantity of plant released volatile organic compounds (VOCs) related to nitrogen concentrations of the plant. In this study, we determined the infestation behavior of B. tabaci to the tomato plants that produced different levels of VOCs after application of different levels of nitrogen with a wind tunnel and an olfactometer. We also analyzed the VOCs released from nitrogen-treated tomato plants using solid phase microextraction and gas chromatography-mass spectrometry. The results revealed that the production of eight VOCs (β-pinene, (+)-4-carene, α-terpinene, p-cymene, β-phellandrene, α-copaene, β-caryophyllene, and α-humulene) was reduced after the plants were treated with high levels of nitrogen. However, more whiteflies were attracted to the tomato plants treated with high levels of nitrogen than to the plants treated with normal or below normal levels of nitrogen. These results clearly indicated that nitrogen can change the quality and quantity of tomato plant volatile chemicals, which play important roles in B. tabaci host plant selection. PMID:28408917

  10. Acanthaleyrodes elevatus sp. n. (Hemiptera: Aleyrodidae) from India, with key to species and discussion of tuberculate setae.

    Science.gov (United States)

    Dubey, Anil Kumar; Singh, Sudhir; Martin, Jon H

    2014-11-03

    The genus Acanthaleyrodes Takahashi is reported for the first time from India. Acanthaleyrodes elevatus sp. n. is described from Bridelia retusa in Kerala, India, with a key to puparia of Acanthaleydes species. The new species differs in its exceptionally elevated eighth abdominal tergite, circular vasiform orifice and eight pairs of subdorsal setae. The generic characteristics of Acanthaleyrodes are redefined and distinguished from those of Tuberaleyrodes Takahashi. A. styraci Takahashi is re-described in detail with illustrations of puparia and immatures from Hong Kong. A lectotype puparium is designated for A. callicarpae Takahashi. The development of tuberculate setae is discussed, in whitefly puparia and earlier nymphal instars, and considered to be subject to environmental modification.

  11. Plan de muestreo directo para Trialeurodes vaporariorum (Westwood (Hemiptera: Aleyrodidae en cultivos comerciales de tomate

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    Bernal Liliana

    2008-08-01

    Full Text Available

    Los daños ocasionados por Trialeurodes vaporariorum generan pérdidas económicas que disminuyen la producción y pueden contribuir en el alza de los precios del tomate. Para su control se acude a aplicaciones continuas de insecticidas, sin obtener resultados satisfactorios. Como alternativa de control se realizan liberaciones del parasitoide E. formosa. Sin embargo, el éxito de este sistema implica estimar la densidad de la plaga a través de un método de muestreo adecuado. Por ello, se estandarizó un plan de muestreo directo, económicamente viable, para ninfas de mosca blanca en un cultivo comercial de tomate bajo invernadero. Se determinó la incidencia de prácticas de manejo y de la fenología en la distribución espacial de ninfas. En términos generales, el muestreo se dividió en tres etapas: 1 muestreo totalmente aleatorio, entre las 5 y 7 semanas después de la siembra (sds, para plantas no divididas en tercios; 2 muestreo aleatorio, entre las 8 y 16 sds, los conteos se realizaron en hojas del tercio inferior; 3 muestreo bietápico: planta y hoja, entre las 17 y 24 sds, los conteos se efectuaron sobre hojas de los tercios medio y superior. Se encontró que prácticas culturales como el despunte influyeron en la distribución espacial de las ninfas. La implementación del muestreo directo generó un incremento en la producción del 0,06% y permitió estimar la densidad de ninfas de Trialeurodes vaporariorum en el cultivo (con precisiones de 5%, 10% y 25% como criterio para liberar E. formosa.

  12. Proximate Composition, Nutritional Attributes and Mineral Composition of <em>Peperomia> <em>pellucida> L. (Ketumpangan Air Grown in Malaysia

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    Maznah Ismail

    2012-09-01

    Full Text Available This study presents the proximate and mineral composition of <em>Peperomia> <em>pellucida> L., an underexploited weed plant in Malaysia. Proximate analysis was performed using standard AOAC methods and mineral contents were determined using atomic absorption spectrometry. The results indicated <em>Peperomia> <em>pellucida> to be rich in crude protein, carbohydrate and total ash contents. The high amount of total ash (31.22% suggests a high-value mineral composition comprising potassium, calcium and iron as the main elements. The present study inferred that <em>Peperomia> <em>pellucida> would serve as a good source of protein and energy as well as micronutrients in the form of a leafy vegetable for human consumption.

  13. Three new species of mealybug (Hemiptera, Coccomorpha, Pseudococcidae) on persimmon fruit trees (Diospyros kaki) in southern Brazil.

    Science.gov (United States)

    Pacheco da Silva, Vitor C; Kaydan, Mehmet Bora; Germain, Jean-François; Malausa, Thibaut; Botton, Marcos

    2016-01-01

    Brazil has the greatest insect diversity in the world; however, little is known about its scale insect species (Hemiptera: Coccomorpha). Mealybugs (Pseudococcidae) have been found in at least 50% of persimmon orchards Diospyros kaki L. in the southern part of the country. In this study three new mealybug species on persimmon trees located in the Serra Gaúcha Region, RS, Brazil, namely, Anisococcus granarae Pacheco da Silva & Kaydan, sp. n., Ferrisia kaki Kaydan & Pacheco da Silva, sp. n. and Pseudococcus rosangelae Pacheco da Silva & Kaydan, sp. n. are described. In addition, an identification key for the genera occurring on fruit orchards and vineyards in Brazil is provided, together with illustrations and molecular data for the new species.

  14. Evaluation of Antioxidant Activities of Aqueous Extracts and Fractionation of Different Parts of <em>Elsholtzia em>ciliata>

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    Yuangang Zu

    2012-05-01

    Full Text Available The aim of this study was to investigate the antioxidant and free-radical scavenging activity of extract and fractions from various parts of <em>Elsholtzia ciliataem>. The inflorescences, leaves, stems and roots of <em>E. ciliataem> were extracted separately and two phenolic component enrichment methods: ethyl acetate-water liquid-liquid extraction and macroporous resin adsorption-desorption, were adopted in this study. The antioxidant activities of water extracts and fractions of <em>E. ciliataem> were examined using different assay model systems <em>in vitroem>. The fraction root E (purified by HPD300 macroporous resin exhibited the highest total phenolics content (497.2 ± 24.9 mg GAE/g, accompanied with the highest antioxidant activity against various antioxidant systems <em>in vitroem> compared to other fractions. On the basis of the results obtained, <em>E. ciliataem> extracts can be used potentially as a ready accessible and valuable bioactive source of natural antioxidants.

  15. Ingestão de seiva do xilema de laranjeiras 'Pêra' e 'Valência' (Citrus sinensis (L. Osbeck sadias e infectadas por Xylella fastidiosa, pelas cigarrinhas vetoras Oncometopia facialis e Dilobopterus costalimai (Hemiptera: Cicadellidae Xylem sap ingestion form healthy "Pera" and "Valencia" sweet orange (Citrus sinensis (L. Osbeck and infected ones by Xylella fastidiosa, Oncometopia facialis and Dilobopterus costalimai (Hemiptera: Cicadellidae

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    Luiz Henrique Montesino

    2006-08-01

    Full Text Available Estudou-se o efeito da infecção pela bactéria Xylella fastidiosa, agente causal da Clorose Variegada dos Citros (CVC, sobre a taxa de ingestão de seiva do xilema de plantas cítricas por duas espécies de cigarrinhas vetoras (Hemiptera: Cicadellidae. Foram utilizados pés-francos de laranjeira-doce (Citrus sinensis das variedades 'Pêra' e 'Valência', infectadas por X. fastidiosa da linhagem 9a5c, por meio de inoculação mecânica. Os insetos utilizados nos experimentos foram coletados em campo, sendo um representante da Tribo Cicadellini (Dilobopterus costalimai e um da Proconiini (Oncometopia facialis. A taxa de ingestão de seiva do xilema por O. facialis foi quantificada nos ramos das plantas e a de D. costalimai nas folhas e ramos, por meio da avaliação do volume do líquido (honeydew excretado por unidade de tempo. O consumo pela cigarrinha O. facialis nas plantas doentes foi menor do que nas plantas sadias. Na variedade 'Pêra' doente, o consumo foi baixo, não permitindo a quantificação da seiva eliminada. Na 'Pêra' sadia e na 'Valência' doente e sadia, O. facialis apresentou valores expressivos de excreção, com maior alimentação no período diurno. Nas plantas sadias das duas variedades, o consumo pela cigarrinha D. costalimai foi maior do que nas plantas com CVC. Comparando-se as variedades, o consumo foi superior na variedade 'Valência', e, em relação às partes da planta, folha e ramo, a taxa de ingestão foi maior no ramo das duas variedades, apresentando consumo maior no período diurno.It was studied the effect of Xylella fastidiosa infection, causal agent of Citrus Variegated Chlorosis (CVC, on the xylem sap ingestion rate of citrus plants by two sharpshooters species (Hemiptera: Cicadellidae. Seedlings of sweet orange Pera and Valencia (Citrus sinensis were used and infected by X. fastidiosa, strain 9a5c, obtained by mechanical inoculation. The insects used in the experiments were collected in the field, one

  16. Tabela de vida de fertilidade de Lysiphlebus testaceipes (Cresson (Hymenoptera, Braconidae, Aphidiinae em Rhopalosiphum maidis (Fitch e Aphis gossypii Glover (Hemiptera, Aphididae Fertility life table of Lysiphlebus testaceipes (Cresson (Hymenoptera, Braconidae, Aphidiinae in Rhopalosiphum maidis (Fitch and Aphis gossypii Glover (Hemiptera, Aphididae

    Directory of Open Access Journals (Sweden)

    Robson José da Silva

    2008-01-01

    Full Text Available Avaliar o potencial de crescimento de Lysiphlebus testaceipes (Cresson é importante para seu uso em programas de controle biológico de pulgões. Este trabalho teve como objetivo determinar a tabela de vida de fertilidade de L. testaceipes em Rhopalosiphum maidis (Fitch e Aphis gossypii Glover. Na avaliação da mortalidade de imaturos, do desenvolvimento e da razão sexual foram utilizadas 12 fêmeas do parasitóide e 480 ninfas de cada pulgão testado. Na avaliação da longevidade e da fertilidade foram utilizadas 15 fêmeas do parasitóide e uma colônia por dia de cada pulgão, até a morte da fêmea do parasitóide, sendo 300 (1º dia; 250 (2º dia; 200 (3º dia; 150 (4º dia; 100 (5º dia e 50 ninfas nos demais dias. L. testaceipes apresentou taxas de mortalidade de imaturos de 5,6% em R. maidis e de 9,2% em A. gossypii, desenvolvimento de 10,2 e 10,1 dias e razão sexual de 0,71 e 0,66, respectivamente. L. testaceipes apresentou fecundidade de 498,2 ovos em R. maidis e de 327,8 ovos em A. gossypii. Os parâmetros de crescimento de L. testaceipes em R. maidis e A. gossypii foram, respectivamente, R O= 205,38 e 164,08 fêmeas; r m= 0,449 e 0,431 fêmeas/fêmeas/dia; lambda = 1,57 e 1,54 fêmeas/dia; T= 11,86 e 11,83 dias e TD= 10,78 e 11,27 dias. L. testaceipes apresenta alto potencial de crescimento em R. maidis e A. gossypii. R. maidis mostrou ser hospedeiro adequado aos propósitos de criação massal e utilização em sistema de criação aberta para L. testaceipes.The evaluation of the growth potential of Lysiphlebus testaceipes (Cresson is important for its use in biological control programs of aphids. This work aimed to evaluate the fertility life table of L. testaceipes in Rhopalosiphum maidis (Fitch and Aphis gossypii Glover. To determine the immature mortality, development and the sex ratio of the parasitoid, 12 females parasitoid, and 480 nymphs of each aphids were used. To evaluate the longevity and fertility 15 female

  17. First occurrence of Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae) preying on defoliating caterpillars of oil palm in the state of Para, Brazil

    International Nuclear Information System (INIS)

    Ribeiro, Rafael C.; Lemos, Walkymario P.; Muller, Antonio A.

    2010-01-01

    The oil palm Elaeis guineensis is usually attacked by pests, particularly, defoliating caterpillars. Between 2004 and 2006 a stinkbug predator (Asopinae) was registered preying on caterpillars of Brassolis sophorae L., Opsiphanes invirae Hubner (Lepidoptera: Nymphalidae) and Sibine spp. (Lepidoptera: Limacodidae), reducing their populations in commercial oil palm plantations in the State of Para, Brazil. Specimens of the natural enemy were collected, mounted, and identified as Alcaeorrhynchus grandis (Dallas) (Hemiptera: Pentatomidae), corresponding to the first report of the occurrence of this stinkbug attacking defoliating caterpillars of oil palm in Brazil. (author)

  18. Ultrastructural studies of the hemocytes of Panstrongylus megistus (Hemiptera: Reduvidae Ultra-estrutura dos hemócitos de Panstrongylus megistus (Hemiptera: Reduviidae

    Directory of Open Access Journals (Sweden)

    Margherita A. Barracco

    1989-06-01

    Full Text Available Ultrastructural analyses revealed the presence of six hemocyte types in the hemolymph of Panstrogylus megistus, partially confirming our previous results obtained through light microscopy. Prohemocytes: small, round hemocytes with a thin cytoplasm layer, espcieally rich in free ribosomes and poor in membranous systems. Plasmatocytes: polymorphic cells, whose cytoplasm contains many lysosomes and a well developed rough endoplasmic reticulum (RER.They are extremely phagocytic. Sometimes, they show a large vacuolation. Granulocytes: granular hemocytes whose granules show different degrees of electrondensity. Most of them, have an internal structuration. Coagulocytes: oval or elongated hemocytes, which show pronounced perinuclear cisternae as normally observed in coagulocytes. The cytoplasm is usually electrondense, poor in membranous systems and contains many labile granules. Oenocytoids: large and very stable hemocytes, whose homogeneous cytoplasme is rich in loose ribosomes and poor in membranous systems. Adipohemocytes: large cells, containing several characteristic lipid droplets. The cytoplasm is also rich in glycogen, RER and large mitochondria. The total and differential hemocyte count (THC and DHC were also calculated for this reduviid. THC increases from 2,900 hemocytes/cubic millimeter of hemolymph in the 4th intar to 4,350 in the 5th and then, decreases to 1,950 in the adults. Plasmatocytes and coagulocytes are the predominant hemocyte types.Estudos ao microscópio eletrônico de transmissão revelaram a presença de seis tipos de hemócitos na hemolinfa de Panstrongylus megistus. Estes resultados confirmam parcialmente os obtidos anteriormente através da microscopia de luz. Pró-hemócitos: células pequenas e arredondadas, cuja delgada faixa citoplasmática é especialmente rica em ribossomos livres e pobre em sistemas membranosos. Plasmatócitos: células polimórficas, cujo citoplasma caracteriza-se por um retículo endoplasm

  19. Oviposition behavior of the silver leaf whitefly Bemisia tabaci biotype B on tomato; Comportamento de oviposicao da mosca-branca Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) biotipo B em tomateiro

    Energy Technology Data Exchange (ETDEWEB)

    Vendramim, Jose D. [Universidade de Sao Paulo (USP), Piracicaba, SP (Brazil). Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Dept. de Entomologia, Fitopatologia e Zoologia Agricola]. E-mail: jdvendra@esalq.usp.br; Souza, Antonio P. de [Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS (Brazil). Dept. de Morfofisiologia. Lab. de Anatomia Humana]. E-mail: apsouza@nin.ufms.br; Ongarelli, Maria das G. [Universidade de Sao Paulo (USP), Piracicaba, SP (Brazil). Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Lab. de Fisiologia e Bioquimica Pos-Colheita]. E-mail: mgong@esalq.usp.br

    2009-01-15

    The objective of this work was to evaluate the influence of the leaf surface, the insect geotropic behavior and the type of foliar trichome on Bemisia tabaci (Genn.) biotype B oviposition on tomato leaves. Bemisia tabaci females were confined in acrylic boxes in which tomato leaflets were fixed at the bottom and top part of the boxes to allow insects to access the leaf surface to be tested (adaxial and/or abaxial) in both no-choice and free choice tests. Oviposition was always higher when the leaf was offered at the top of the box and preferably at the abaxial leaf surface. The effects of leaf trichomes (glandular and non glandular) on B. tabaci oviposition was evaluated by offering the abaxial surface of tomato leaflets to females after a 70% ethanol wash to remove glandular exsudates against a control treatment (without a ethanol wash). Oviposition was concentrated mostly near to non glandular trichomes, showing whitefly females can discriminate among the trichomes. (author)

  20. Allometric and Isometric variations in the Italian <em>Apodemus sylvaticusem> and <em>Apodemus flavicollisem> with respect to the conditions of allopatry and sympatry / Variazioni allometriche e isometriche in <em>Apodemus sylvaticusem> e <em>Apodemus flavicollisem> italiani, rispetto alle condizioni di allopatria e simpatria

    Directory of Open Access Journals (Sweden)

    Giovanni Amori

    1986-12-01

    Full Text Available Abstract In Italy there are two species of <em>Apodemus> (<em>Sylvaemus>: <em>Apodemus sylvaticusem> on the mainland and the main island, and <em>Apodemus flavicollisem> only on the mainland. The trend of some morphometric characters of the skull (incisive foramen length - FI; interorbital breadth = IO; length of palatal bridge = PP; upper alveolar length = $M^1M^3$ was analized and some theoretical models verified for <em>A. sylvaticusem>. If one considers the sympatric population of <em>A. sylvaticusem> and <em>A. flavicollisem> simultaneously the characters PP, IO and $M^1M^3$ appear significantly isometric being directly correlated ($P leq O.O1$, while FI character results allometric with respect to the previous ones, as expected. If one considers the sympatric populations of each of the species separately, the scenario is different. For <em>A. sylvaticusem> only PP and $M^1M^3$ are isometric ($P leq 0.05$. For <em>A. flavicollisem> only $M^1M^3$ and FI appear to be correlated, although not as significantly as for <em>A. sylvaticusem> ($P le 0.05$; one tail. The insular populations of <em>A. sylvaticusem> do not show significant correlations, except for FI and $M^1M^3$ ($P le 0.05$. On the contrary, considering all populations, sympatric and allopatric, of <em>A. sylvaticusem> at the same time are significant correlations ($P le 0.05$ in all combinations of characters, except for those involving the IO. We suggest that the isometric relations in sympatric assemblages are confined within a morphological range available to the genus <em>Apodemus>. In such a space, the two species are split in two different and innerly homogeneous distributions. We found no evidence to confirm the niche variation hypothesis. On the contrary, the variability expressed as SO or CV's appears higher in the sympatric populations than in the allopatric ones, for three of the four characters, confirming previous results

  1. Does the aggressiveness of the prey modify the attack behavior of the predator Supputius cincticeps (Stål (Hemiptera, Pentatomidae?

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    Rafael Braga da Silva

    2012-06-01

    Full Text Available Does the aggressiveness of the prey modify the attack behavior of the predator Supputius cincticeps (Stål (Hemiptera, Pentatomidae? The stink bug Supputius cincticeps (Stål (Hemiptera, Pentatomidae is a predator found in several Brazilian regions, which possesses desirable attributes as a natural control agent and in biological control programs. The aim of this study was to test if the attack behavior and predation success of S. cincticeps were affected by prey species. Larvae of Tenebrio molitor (L. (Coleoptera, Tenebrionidae, Spodoptera frugiperda (J. E. Smith (Lepidoptera, Noctuidae, and Thyrinteina arnobia (Stoll (Lepidoptera, Geometridae were offered to S. cincticeps in laboratory bioassays where predatory attack and prey defensive behaviors were observed for 2-hour periods. The attack behavior of S. cincticeps changed with the prey species offered. More than 25% of T. molitor and S. frugiperda larvae were immediately attacked, but T. arnobia was not immediately attacked by S. cincticeps. Successful attack (i.e., successful insertion of the predator stylets into the prey depends on the region of the body attacked, with a greater proportion of successful attacks in the anterior than in the median or posterior regions. Larvae of T. arnobia and S. frugiperda displayed a sequence of abrupt head and body movements in response to S. cincticeps attack. Attempts of predation were more successful on T. molitor and S. frugiperda than on T. arnobia. Information about the differential attack behavior of S. cincticeps on different prey species is important for designing successful biological control programs using this hemipteran predator.

  2. Bionomy of the laurel scale Aonidia lauri (Bouche (Hemiptera: Diaspididae in Podgorica, Montenegro

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    Graora Draga

    2016-01-01

    Full Text Available Aonidia lauri (Bouche (Hemiptera: Diaspididae is a serious pest of laurel (Laurus nobilis L. in urban parts of the City of Podgorica (Montenegro. Severe infestation causes chlorotic spots and necrotic rings around feeding spots, drying and dieback of leaves and buds. In addition, plants become physiologically weak and lose in aesthetic quality, while continuous infestation in urban areas often leads to partial or complete drying of plants. This study of the biology of A. lauri on L. nobilis was carried out at three locations in Podgorica ('Stara Varoš', 'Centar' and 'Preko Morače', Montenegro, in 2010 and 2011. A. lauri developed three generations annually and overwintered on laurel leaves and branches as the second-instar nymph-larval stage. An extended period of larval development ensures a continuous presence of all development stages on plants, which leads to overlapping of generations. Sporadic predatory ladybirds, Chilocorus bipustulatus (L. (Coleoptera: Coccinellidae, whose larvae and adults feed on scales, were detected inside A. lauri colonies.

  3. Probing behaviors of Sitobion avenae (Hemiptera: Aphididae on enhanced UV-B irradiated plants

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    Hu Zu-Qing

    2013-01-01

    Full Text Available UV-B induced changes in plants can influence sap-feeding insects through mechanisms that have not been studied. Herein the grain aphid, Sitobion avenae (Fabricius (Hemiptera: Aphididae, was monitored on barley plants under the treatments of control [0 kJ/ (m2.d], ambient UV-B [60 kJ/ (m2.d], and enhanced UV-B [120 kJ/ (m2.d] irradiation. Electrical penetration graph (EPG techniques were used to record aphid probing behaviors. Enhanced UV-B irradiated plants negatively affected probing behaviors of S. avenae compared with control plants. In particular, phloem factors that could diminish sieve element acceptance appeared to be involved, as reflected by smaller number of phloem phase, shorter phloem ingestion, and fewer aphids reaching the sustained phloem ingestion phase (E2>10min. On the other hand, factors from leaf surface, epidermis, and mesophyll cannot be excluded, as reflected by higher number of non-probing, longer non-probing and pathway phase, and later the time to first probe.

  4. A Novel Apoptosis Correlated Molecule: Expression and Characterization of Protein Latcripin-1 from <em>Lentinula em>edodes> C91–3

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    Min Huang

    2012-05-01

    Full Text Available An apoptosis correlated molecule—protein Latcripin-1 of <em>Lentinula> edodesem> C91-3—was expressed and characterized in <em>Pichia pastorisem> GS115. The total RNA was obtained from <em>Lentinula edodesem> C91–3. According to the transcriptome, the full-length gene of Latcripin-1 was isolated with 3'-Full Rapid Amplification of cDNA Ends (RACE and 5'-Full RACE methods. The full-length gene was inserted into the secretory expression vector pPIC9K. The protein Latcripin-1 was expressed in <em>Pichia pastorisem> GS115 and analyzed by Sodium Dodecylsulfonate Polyacrylate Gel Electrophoresis (SDS-PAGE and Western blot. The Western blot showed that the protein was expressed successfully. The biological function of protein Latcripin-1 on A549 cells was studied with flow cytometry and the 3-(4,5-Dimethylthiazol-2-yl-2,5-Diphenyl-tetrazolium Bromide (MTT method. The toxic effect of protein Latcripin-1 was detected with the MTT method by co-culturing the characterized protein with chick embryo fibroblasts. The MTT assay results showed that there was a great difference between protein Latcripin-1 groups and the control group (<em>p em>< 0.05. There was no toxic effect of the characterized protein on chick embryo fibroblasts. The flow cytometry showed that there was a significant difference between the protein groups of interest and the control group according to apoptosis function (<em>p em>< 0.05. At the same time, cell ultrastructure observed by transmission electron microscopy supported the results of flow cytometry. The work demonstrates that protein Latcripin-1 can induce apoptosis of human lung cancer cells A549 and brings new insights into and advantages to finding anti-tumor proteins.

  5. Análise faunística e flutuação populacional de cigarrinhas (Hemiptera, Cicadellidae em pomar cítrico no município de Chapecó, Santa Catarina

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    Angela Camila Orbem Menegatt

    2008-01-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2008v21n1p53 Visando caracterizar os ecossistemas de pomares cítricos da Região Oeste de Santa Catarina em relação às espécies de Cicadellidae, desenvolveu-se uma análise faunística em Chapecó. Para a caracterização do município foram medidos os índices de abundância, constância, dominância e freqüência das moscas das frutas. O levantamento foi feito utilizando-se armadilhas do tipo bandejas amarelas. Dilobopterus costalimai, Acrogonia citrina e Scopogonalia subolivacea foram as espécies mais abundantes, constantes, freqüentes e dominantes, podendo ser consideradas como predominantes. Dilobopterus costalimai apresentou dois picos populacionais, um em agosto e outro em dezembro e janeiro. Acrogonia citrina apresentou dois picos populacionais, um em agosto e outro em janeiro e fevereiro e Scopogonalia subolivacea apresentou um único pico populacional do mês de dezembro e janeiro.

  6. A new genus and species of tettigarctid cicada from the early Miocene of New Zealand: Paratettigarcta zealandica (Hemiptera, Auchenorrhyncha, Tettigarctidae)

    Science.gov (United States)

    Kaulfuss, Uwe; Moulds, Max

    2015-01-01

    Abstract A new genus and species of primitive cicada (Hemiptera: Tettigarctidae) is described from the early Miocene of southern New Zealand. Paratettigarcta zealandica gen. et sp. n. is the first cicada (Cicadoidea) fossil from New Zealand and exhibits wing venation patterns typical for the subfamily Tettigarctinae. It differs from other fossil taxa and the extant genus Tettigarcta in the early divergence of CuA2 from the nodal line in the forewing, its parallel-sided subcostal cell, the early bifurcation of vein M and long apical cells of the hindwing, and in wing pigmentation patterns. PMID:25829843

  7. Nymphal and adult performance of Euschistus heros (Fabr.) (Hemiptera: Pentatomidae), as a potential alternative host for egg parasitoids multiplication

    International Nuclear Information System (INIS)

    Peres, Wilsimar A.A.; Correa-Ferreira, Beatriz S.

    2001-01-01

    This research aimed to evaluate the potential of Euschistus heros (Fabr.) (Hemiptera: Pentatomidae) as host for multiplication of egg parasitoids, by determining the nymphal and adult performance of E. heros from laboratory and the field, comparing with Nezara viridula (L.) (Hemiptera: Pentatomidae), under mass conditions. One hundred eggs of E. heros and N. viridula were placed among the leaves of soybean plants contained in cages (50x50x70 cm) and observation were made until adult emergence. The nymphs fed on soybean pods, dry soybean and peanuts seeds. The number of nymphs that reached adulthood and the development time were calculated. The survivorship and reproduction performance of laboratory and field populations of E. heros and N. viridula were evaluated during 13 weeks in February-May 1999. The number of eggs produced by 100 pairs of stink bugs per cage containing the same diet was recorded. Nymphal development time of E. heros and N. viridula was 33.0 and 34.0 days and 65.0% and 71.3% of nymphs reached adulthood, respectively. Adults of E. heros reared under laboratory conditions produced 2.5 times more eggs (5547.0 eggs/cage) than those collected in the field (2262.7 eggs/cage). The adult field population of E. heros had reduced reproduction and longevity due to parasitism by Hexacladia smithii Ash. (Hymenoptera: Encyrtidae). The N. viridula adults collected in the field produced 1.7 times more eggs (6304.9 eggs/cage) than those reared in the laboratory (3609.2 eggs/cage). E. heros laboratory reared is a promising host for egg parasitoids multiplication when compared with N. viridula collected in the field. (author)

  8. Nymphal and adult performance of Euschistus heros (Fabr.) (Hemiptera: Pentatomidae), as a potential alternative host for egg parasitoids multiplication

    Energy Technology Data Exchange (ETDEWEB)

    Peres, Wilsimar A.A. [Universidade Federal do Parana, Curitiba, PR (Brazil). Dept. de Zoologia; Correa-Ferreira, Beatriz S. [EMBRAPA, Londrina, PR (Brazil). Centro Nacional de Pesquisa de Soja

    2001-12-15

    This research aimed to evaluate the potential of Euschistus heros (Fabr.) (Hemiptera: Pentatomidae) as host for multiplication of egg parasitoids, by determining the nymphal and adult performance of E. heros from laboratory and the field, comparing with Nezara viridula (L.) (Hemiptera: Pentatomidae), under mass conditions. One hundred eggs of E. heros and N. viridula were placed among the leaves of soybean plants contained in cages (50x50x70 cm) and observation were made until adult emergence. The nymphs fed on soybean pods, dry soybean and peanuts seeds. The number of nymphs that reached adulthood and the development time were calculated. The survivorship and reproduction performance of laboratory and field populations of E. heros and N. viridula were evaluated during 13 weeks in February-May 1999. The number of eggs produced by 100 pairs of stink bugs per cage containing the same diet was recorded. Nymphal development time of E. heros and N. viridula was 33.0 and 34.0 days and 65.0% and 71.3% of nymphs reached adulthood, respectively. Adults of E. heros reared under laboratory conditions produced 2.5 times more eggs (5547.0 eggs/cage) than those collected in the field (2262.7 eggs/cage). The adult field population of E. heros had reduced reproduction and longevity due to parasitism by Hexacladia smithii Ash. (Hymenoptera: Encyrtidae). The N. viridula adults collected in the field produced 1.7 times more eggs (6304.9 eggs/cage) than those reared in the laboratory (3609.2 eggs/cage). E. heros laboratory reared is a promising host for egg parasitoids multiplication when compared with N. viridula collected in the field. (author)

  9. Effects of insecticides used in Bemisia tabaci (Gennadius biotype B control and their selectivity to natural enemies in soybean cropEfeitos dos inseticidas utilizados no controle de Bemisia tabaci (Gennadius biótipo B e sua seletividade aos inimigos naturais na cultura da soja

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    Simone Silva Vieira

    2012-10-01

    Full Text Available In recent crop seasons, the whitefly, Bemisia tabaci biotype B has become a serious pest in soybean crops due to high infestations and its control difficulties. Therefore, it is important to search control alternatives in the integrated pest management approach. Thus, it was evaluated in this study the efficacy of the whitefly control using different insecticides in greenhouse conditions and their selectivity to the parasitoids Encarsia formosa, Trichogramma pretiosum and Telenomus remus. Buprofezin 150 g. a.i.ha-1 + mineral oil 0.2% v/v and pyriproxyfen 100 g. a.i.ha-1 were considered the best options for the whitefly management due to combine good pest control efficacy with higher selectivity to the parasitoids except Encarsia formosa for which no treatment was classified as harmless. Betacyflutrin 9.375 + imidacloprid 75 g. i.a. ha-1 was efficient on controlling whiteflies nymphs but was not harmless to the studied natural enemies. In general, the treatments including pyretroids compounds (betacyflutrin 9.375 + imidacloprid 75 + spiromesifen 60, betacyflutrin 9.375 + imidacloprid 75 and lambda-cyhalothrin 26.5 + thiametoxan 35.25 g. a.i. ha-1 were the most harmful to the evaluated parasitoids and therefore it use should be avoid whenever possible. Nas últimas safras a mosca-branca, Bemisia tabaci biótipo B (Hemiptera: Aleyrodidae tornou-se praga de importância econômica para a cultura da soja devido à ocorrência de altas infestações e à dificuldade de controle desta praga. Sendo assim, é importante buscar alternativas de manejo com base em um programa de manejo integrado de pragas. Assim, avaliaram-se neste trabalho a eficiência de diferentes inseticidas no controle da mosca-branca em casa-de-vegetação e a seletividade destes aos parasitoides Encarsia formosa, Trichogramma pretiosum e Telenomus remus. Buprofezina 150 g i.a. ha-1 + óleo mineral 0,2% v/v e piriproxifem 100 g. i.a. ha-1 foram consideradas as melhores opções para

  10. An annotated checklist of scale insects (Hemiptera: Coccoidea) of Saint Lucia, Lesser Antilles .

    Science.gov (United States)

    Malumphy, Chris

    2014-07-31

    An annotated list of 83 scale insect species (Hemiptera: Sterorrhyncha: Coccoidea) recorded from Saint Lucia is presented, based on data gathered from UK quarantine interceptions, samples collected in an urban coastal habitat in the North West of the Island in 2013, and published records. Thirty-three species (40%) are recorded for the first time for the country, including Dysmicoccus joannesiae (Costa Lima), a South American mealybug, and Poliaspoides formosana (Takahashi), an Asian armoured scale insect pest of bamboo, which are new for the Caribbean region. The economic, environmental and social impacts caused by introduced exotic species of scale insect are discussed. Two predatory midges Diadiplosis ?coccidivora (Felt) and Diadiplosis multifila (Felt) (Diptera: Cecidomyiidae) are recorded for the first time from Saint Lucia. The latter species was observed causing 90% mortality of a large infestation of passion vine mealybug Planococcus minor (Maskell) on soursop fruit. 

  11. Momentos em freios e em embraiagens

    OpenAIRE

    Mimoso, Rui Miguel Pereira

    2011-01-01

    Dissertação para obtenção do Grau de Mestre em Mestrado Integrado em Engenharia Mecânica Nesta dissertação reúnem-se os modelos de cálculo utilizados na determinação dos momentos em freios e em embraiagens. Neste trabalho consideram-se os casos de freios e embraiagens de atrito seco e atrito viscoso. Nos freios de atrito viscoso são considerados casos em que as características dos fluidos não são induzidas, e outros em que são induzidas modificações a essas mesmas características. São a...

  12. A new iranian species of Uroleucon Mordvilko, 1914 (Hemiptera, Aphididae from specimens in the Natural History Museum collection

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    Nieto Nafría, Juan M.

    2014-06-01

    Full Text Available Uroleucon (Uromelan helichrysi sp. n. (Hemiptera, Aphididae, Aphidinae: Macrosiphini is established from Iranian apterous and alate viviparous females caught on Helichrysum sp. (Asteraceae and preserved in the collection of the Natural History Museum (BMNH in London. The number of setae on the first tarsal segments (5, the number of caudal setae (20 to 28, the presence of abdominal marginal tubercles on abdominal segments 2 to 4, and the size of the cells of the siphuncular reticulation (relatively small allow the new species to be distinguished from other Palaeartic species of the subgenus Uromelan. Blackman and Eastop’s key to apterae on Helichrysum is modified to include the new species. urn:lsid:zoobank.org:pub:E2B89D59-05FD-4A9E-AD78-B3C302219A10Se establece la especie Uroleucon helichrysi n. sp. (Hemiptera: Aphididae: Aphidinae: Macrosiphini con la descripción de sus hembras vivíparas ápteras y aladas recogidas en Irán sobre Helichrysum sp. (Asteraceae, conservadas en la colección del Natural History Museum de Londres. La nueva especie se diferencia de otras especies paleárticas del subgénero Uromelan por la cantidad de setas en el primer segmento tarsal (5, la cantidad de setas caudales (20 a 28, por el tamaño de las celdillas de la reticulación cornicular (que son relativamente pequeñas y por la presencia de papilas marginales (presentes en los segmentos abdominales 2 a 4 Se incluye una modificación a la clave de Blackman and Eastop para las ápteras que viven sobre Helichrysum. urn:lsid:zoobank.org:pub:E2B89D59-05FD-4A9E-AD78-B3C302219A10

  13. Comparison of Relative Bias, Precision, and Efficiency of Sampling Methods for Natural Enemies of Soybean Aphid (Hemiptera: Aphididae).

    Science.gov (United States)

    Bannerman, J A; Costamagna, A C; McCornack, B P; Ragsdale, D W

    2015-06-01

    Generalist natural enemies play an important role in controlling soybean aphid, Aphis glycines (Hemiptera: Aphididae), in North America. Several sampling methods are used to monitor natural enemy populations in soybean, but there has been little work investigating their relative bias, precision, and efficiency. We compare five sampling methods: quadrats, whole-plant counts, sweep-netting, walking transects, and yellow sticky cards to determine the most practical methods for sampling the three most prominent species, which included Harmonia axyridis (Pallas), Coccinella septempunctata L. (Coleoptera: Coccinellidae), and Orius insidiosus (Say) (Hemiptera: Anthocoridae). We show an important time by sampling method interaction indicated by diverging community similarities within and between sampling methods as the growing season progressed. Similarly, correlations between sampling methods for the three most abundant species over multiple time periods indicated differences in relative bias between sampling methods and suggests that bias is not consistent throughout the growing season, particularly for sticky cards and whole-plant samples. Furthermore, we show that sticky cards produce strongly biased capture rates relative to the other four sampling methods. Precision and efficiency differed between sampling methods and sticky cards produced the most precise (but highly biased) results for adult natural enemies, while walking transects and whole-plant counts were the most efficient methods for detecting coccinellids and O. insidiosus, respectively. Based on bias, precision, and efficiency considerations, the most practical sampling methods for monitoring in soybean include walking transects for coccinellid detection and whole-plant counts for detection of small predators like O. insidiosus. Sweep-netting and quadrat samples are also useful for some applications, when efficiency is not paramount. © The Authors 2015. Published by Oxford University Press on behalf of

  14. Pentatomídeos (Hemiptera associados a espécies nativas em Itaara, RS, Brasil

    Directory of Open Access Journals (Sweden)

    Juliana Garlet

    2010-04-01

    Full Text Available http://dx.doi.org/10.5007/2175-7925.2010v23n1p91 Com o objetivo de determinar qualitativamente e quantitativamente os pentatomídeos associados a diferentes espécies botânicas nativas, no período de setembro de 2005 a setembro de 2006, foram coletados insetos em nove espécies: Gochnatia polymorpha (Less. (cambará, Eugenia uniflora Berg. (pitangueira, Acca selowiana (Berg Burret (goiaba-da-serra, Psidium cattleianum Sabine (araçá, Baccharis spp., Solanaum mauritianum Scop. (fumo-bravo, Micanea cinerascens Miq. (passiquinho, Calliandra brevipes Bhent. (caliandra, Schinus molle L. (aroeira, localizadas na barragem Rodolfo da Costa e Silva, município de Itaara, RS. Foram realizadas coletas quinzenais, com o auxílio de um funil cônico confeccionado com folha de flandres (2mm, com 70cm de diâmetro na maior abertura e 63cm de altura. Retirou-se uma amostra por espécie botânica por data de coleta, obtida através de dez sacudidas dos ramos sobre o funil. Realizadas as coletas, o material foi levado ao Laboratório de Entomologia do Departamento de Defesa Fitossanitária da Universidade Federal de Santa Maria, onde foi realizada a triagem e identificação do material. Foram identificadas nove espécies de Pentatomidae. Edessa rufomarginata (De Geer, 1773 foi à espécie com maior ocorrência, seguida de Thyanta humilis Bergroth, 1891. A espécie botânica S. mauritianum foi a que apresentou o maior número de espécies de Pentatomidae coletados, representando 26,9% do total.

  15. Parasitic zoonoses: survey in foxes (<em>Vulpes vulpesem> in the northern Apennines / Zoonosi parassitarie: indagini in volpi (<em>Vulpes vulpesem> dell'Appennino settentrionale

    Directory of Open Access Journals (Sweden)

    Vittorio Guberti

    1991-07-01

    Full Text Available Abstract A parasitological survey on 153 foxes was carried out in the northern Apennines, during the period 1984-1987. The following parasites were identified: <em>Toxocara canisem> (46.4%, <em>Taenia> sp. (17%, <em>Uncinaria stenocephalaem> (11.8%, <em>Mesocestoides lineatusem> (11.1%, <em>Ancylostoma caninumem> (3.9%, <em>Taenia hydatigenaem> (3.3%, <em>Trichuris vulpisem> (3.3%, <em>Dipylidium caninumem> (2.6%, <em>Taenia crassicepsem> (2%. All foxes were negative for <em>Trichinella> sp. A statistical analysis was performed to evaluate differences in the parasitic fauna according to the sex and age classes of the hosts. The role that the fox could have as a reservoir of helminthic zoonoses is discussed. The results are compared with those of similar studies carried out in Italy. Riassunto Nel periodo 1984-1987 è stata condotta un'indagine parassitologica su 153 volpi abbattute nell'Appennino romagnolo. Sono stati reperiti i seguenti parassiti: <em>Toxocara canisem> (46,4%, <em>Taenia> sp. (17%, <em>Uncinaria stenocephalaem> (11,8%, <em>Mesocestoides lineatusem> (11,1%, <em>Ancylostoma caninumem> (3,9%, <em>Taenia hydatigenaem> (3,3%, <em>Trichuris vulpisem> (3,3%, <em>Dipylidium caninumem> (2,6%, <em>Taenia crassicepsem> (2%. Tutte le volpi esaminate sono risultate negative per <em>Trichinella> sp. È stata effettuata l'analisi statistica dei dati per evidenziare eventuali differenze della fauna parassitaria in relazione al sesso e all'età delle volpi. Sulla base dei dati ottenuti viene discussa l'importanza che la Volpe può assumere come serbatoio di zoonosi elmintiche. I risultati acquisiti sono inoltre comparati con quelli ottenuti in analoghe ricerche condotte in Italia.

  16. Dieta de Micoureus demerarae (Thomas (Mammalia, Didelphidae associada às florestas contíguas de mangue e terra firme em Bragança, Pará, Brasil Diet of Micoreus demerarae (Thomas (Mammalia, Didelphidae associated with contiguous forests of mangrove and terra firme in Bragança, Pará, Brazil

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    Marcus E. B. Fernandes

    2006-12-01

    Full Text Available A dieta de Micoureus demerarae (Thomas, 1905 foi estudada em bosques de mangue e terra firme através de amostras estomacais e fecais. O número de indivíduos capturados foi inversamente proporcional à disponibilidade de frutos e insetos, sendo Coleoptera e Hemiptera as ordens de artrópodes mais consumidos e Passifloraceae e Arecaceae os frutos mais ingeridos. Desse modo, tanto a maior variabilidade de frutos como a alta produção destes durante a estação seca, parecem explicar o aumento da captura desses animais nos bosques de terra firme, dos quais são originalmente provenientes. Os itens alimentares sugerem que esta espécie possui uma dieta do tipo onívora, independentemente da sazonalidade ou distribuição dos recursos disponíveis.The diet of Micoureus demerarae (Thomas, 1905 was studied in mangrove and terra firme stands by using stomachal and faecal samples. The number of captured individuals was inversely proportional to availability of fruits and insects, being Coleoptera and Hemiptera the most consumed arthropod orders and Passifloraceae and Arecaceae the most ingested fruits. Thus, either fruits variability or their high yield during the dry season seem to explain the increase of captured animals in the terra firme stands, where they originally come from. The food items suggest that this species has an omnivorous diet, independently of the seasonality or distribution of available resources.

  17. Triatoma ryckmani (Hemiptera: Reduviidae) in the epiphyte Tillandsia xerographica (Bromeliaceae) in the semiarid region of Guatemala.

    Science.gov (United States)

    Marroquín, Ricardo; Monroy, Carlota; Jaenson, Thomas G T

    2004-05-01

    For the first time, the reduviid bug Triatoma ryckmani Zeledón and Ponce (Hemiptera; Reduviidae) was recorded to inhabit the epiphyte Tillandsia xerographica Rohweder (Bromeliaceae) in the semiarid region of Guatemala. These bromeliads grow mainly in drought-resistant trees with rough bark such as Pereskia lychnidiflora (Cactaceae). In our study site, we investigated 30 T. xerographica, and 53 specimens of T. ryckmani were found. Most T. ryckmani (68.5%) were unfed. Ants (Formicidae) were the predominant (92.2%) insect taxon in T. xerographica. Other insects such as Blattidae (3.0%), Reduviidae (T. ryckmani: 2.5%), Blaberidae (2.2%), Gryllidae (0.1%), and Acrididae (0.1%) were recorded in the bromeliads. T. xerographica is illegally commercialized without previous inspection. This may cause accidental introduction of T. ryckmani to houses and to other countries.

  18. <em>In Vivoem> Histamine Optical Nanosensors

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    Heather A. Clark

    2012-08-01

    Full Text Available In this communication we discuss the development of ionophore based nanosensors for the detection and monitoring of histamine levels <em>in vivoem>. This approach is based on the use of an amine-reactive, broad spectrum ionophore which is capable of recognizing and binding to histamine. We pair this ionophore with our already established nanosensor platform, and demonstrate <em>in vitroem> and <em>in vivoem> monitoring of histamine levels. This approach enables capturing rapid kinetics of histamine after injection, which are more difficult to measure with standard approaches such as blood sampling, especially on small research models. The coupling together of <em>in vivoem> nanosensors with ionophores such as nonactin provide a way to generate nanosensors for novel targets without the difficult process of designing and synthesizing novel ionophores.

  19. Variabilità morfologica ed ecologica in <em>Neomys fodiensem> e <em>Neomys anomalusem> nell'Appennino settentrionale

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    Dino Scaravelli

    2003-10-01

    Full Text Available I due <em>Neomys> italiani sono ancora da chiarire dal punto di vista della loro caratterizzazione morfologica e ecologica. Il lavoro prende in considerazione un campione di entrambe le specie proveniente da habitat forestali dell?Appennino settentrionale per i quali sono stati identificati i principali parametri ambientali. Vengono quindi descritte la variabilità dei tratti morfologici delle due specie in aree localizzate nel Parco Nazionale Foreste Casentinesi, Monte Falterona e Campigna nell?Appennino tosco-romagnolo. Risultano di sicuro effetto discriminatorio la maschera facciale, il rapporto piede posteriore/coda e i caratteri cranici. Sulla base dei criteri identificativi si sono realizzate rilevazioni di misure corporee per le due specie e una comparazione degli habitat utilizzati. <em>N. fodiensem> appare unica specie nelle faggete-abetine e dominante nei castagneti, mentre nell?Ontaneta e nelle zone aperte e termofile si registra la sola presenza di <em>N. anomalusem>. Mancano entrambi nei prati cespugliati, nella pecceta e nella cerreta. I gradienti presenti sono quindi illustrati. Non appare una differenza altitudinale nel campione esaminato, posto in stazioni tra i 400 e i 1300 m, ma per entrambe vi sono maggiori riscontri nella fascia tra 700 e 850 m. Nell?analisi multivariata rispetto alle altre specie e alle variabili ambientali si riscontra sempre una discreta correlazione con la presenza di acqua di una certa ampiezza, che comunque è significativa solo per <em>N. fodiensem>, mentre risulta di interesse la positiva correlazione di <em>N. anomalusem> con <em>Apodemus sylvaticusem>.

  20. Echolocation calls and morphology in the Mehelyi’s (<em>Rhinolophus mehelyiem> and mediterranean (<em>R. euryaleem> horseshoe bats: implications for resource partitioning

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    Egoitz Salsamendi

    2006-03-01

    Full Text Available Abstract <em>Rhinolophus euryaleem> and <em>R. mehelyiem> are morphologically very similar species and their distributions overlap extensively in the Mediterranean basin. We modelled their foraging behaviour using echolocation calls and wing morphology and, assuming niche segregation occurs between the two species, we explored how it is shaped by these factors. Resting frequency of echolocation calls was recorded and weight, forearm length, wing loading, aspect ratio and wing tip shape index were measured. <em>R. mehelyiem> showed a significantly higher resting frequency than <em>R. euryaleem>, but differences are deemed insufficient for dietary niche segregation. Weight and forearm length were significantly larger in <em>R. mehelyiem>. The higher values of aspect ratio and wing loading and a lower value of wing tip shape index in <em>R. melehyiem> restrict its flight manoeuvrability and agility. Therefore, the flight ability of <em>R. mehelyiem> may decrease as habitat complexity increases. Thus, the principal mechanism for resource partitioning seems to be based on differing habitat use arising from differences in wing morphology. Riassunto Ecolocalizzazione e morfologia nei rinolofi di Mehely (<em>Rhinolophus mehelyiem> e euriale (<em>R. euryaleem>: implicazioni nella segregazione delle risorse trofiche. <em>Rhinolophus euryaleem> e <em>R. mehelyiem> sono specie morfologicamente molto simili, la cui distribuzione risulta largamente coincidente in area mediterranea. Il comportamento di foraggiamento delle due specie è stato analizzato in funzione delle caratteristiche dei segnali di ecolocalizzazione e della morfologia alare, ed è stata valutata l’incidenza di questi fattori nell’ipotesi di una segregazione delle nicchie. È stata rilevata la frequenza a riposo dei segnali ultrasonori, così come il peso, la lunghezza dell’avambraccio, il carico alare, e due

  1. NEEM OIL ANTIFEEDANT AND INSECTICIDAL EFFECTS ON Oebalus poecilus (HEMIPTERA: PENTATOMIDAE MALES AND FEMALES EFEITO INSETICIDA E DETERRENTE DO ÓLEO DE NIM EM MACHOS E FÊMEAS DE Oebalus poecilus (HEMIPTERA: PENTATOMIDAE

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    Patricia Valle Pinheiro

    2010-10-01

    Full Text Available

    The antifeedant and insecticidal effects of two commercial neem (<em>Azadirachta indicaem> oil formulations (Dalneem and Nim-I-Go to <em>Oebalus poecilusem> were evaluated on irrigated rice. To evaluate the antifeedant effect, both formulations were tested at 1% and 2% (v/v concentration levels. The insecticidal effect was evaluated at 0.5%, 1%, 2%, and 4% (v/v concentrations of Dalneem, by ingestion. Both experiments were conducted with four replications by treatment, containing one panicle and adult insects segregated by gender (two insects per plot for the antifeedant effect and five for the insecticidal effect. Both products were efficient, reducing the damage caused by insects. Insects fed less on the panicles treated with neem oil, causing lower number of feeding sheaths per panicle and lower percentage of damaged grains than the control. Spikelets weight was higher in panicles treated with neem oils. Females caused significantly higher damage than males in controls, for numbers of feeding sheaths and for the feeding deterrence index. Only at the 4% (v/v concentration level, Dalneem caused adult mortality higher than in the control. Results showed that neem oil formulations, at >= 1% (v/v concentration, can be used to reduce the quantitative and qualitative damages caused by <em>O. poecilusem> in lowland rice.

    KEY-WORDS: Small rice stink bug; <em>Azadirachta indicaem>; qualitative damages; <em>Oryza sativaem>;> em

  2. Phytochemical Composition, Antioxidant and Xanthine Oxidase Inhibitory Activities of <em>Amaranthus cruentusem> L. and <em>Amaranthus hybridusem> L. Extracts

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    Jeanne F. Millogo

    2012-06-01

    Full Text Available This paper describes a preliminary assessment of the nutraceutical value of <em>Amaranthus cruentus (A. cruentusem> and <em>Amaranthus hybridus (A. hybridusem>, two food plant species found in Burkina Faso. Hydroacetonic (HAE, methanolic (ME, and aqueous extracts (AE from the aerial parts were screened for <em>in vitroem> antioxidant and xanthine oxidase inhibitory activities. Phytochemical analyses revealed the presence of polyphenols, tannins, flavonoids, steroids, terpenoids, saponins and betalains. Hydroacetonic extracts have shown the most diversity for secondary metabolites. The TLC analyses of flavonoids from HAE extracts showed the presence of rutin and other unidentified compounds. The phenolic compound contents of the HAE, ME and AE extracts were determined using the Folin–Ciocalteu method and ranged from 7.55 to 10.18 mg Gallic acid equivalent GAE/100 mg. Tannins, flavonoids, and flavonols ranged from 2.83 to 10.17 mg tannic acid equivalent (TAE/100 mg, 0.37 to 7.06 mg quercetin equivalent (QE /100 mg, and 0.09 to 1.31 mg QE/100 mg, respectively. The betacyanin contents were 40.42 and 6.35 mg Amaranthin Equivalent/100 g aerial parts (dry weight in <em>A. cruentusem> and <em>A. hybridusem>, respectively. Free-radical scavenging activity expressed as IC50 (DPPH method and iron reducing power (FRAP method ranged from 56 to 423 µg/mL and from 2.26 to 2.56 mmol AAE/g, respectively. Xanthine oxidase inhibitory activities of extracts of <em>A. cruentus em>and <em>A. hybridusem> were 3.18% and 38.22%, respectively.<em> em>The> A. hybridusem> extract showed the best antioxidant and xanthine oxidase inhibition activities. The results indicated that the phytochemical contents of the two species justify their traditional uses as nutraceutical food plants.

  3. Dermatoses em renais cronicos em terapia dialitica

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    Luis Alberto Batista Peres

    2014-03-01

    Full Text Available Objetivo: As desordens cutâneas e das mucosas são comuns em pacientes em hemodiálise a longo prazo. A diálise prolonga a expectativa de vida, dando tempo para a manifestação destas anormalidades. Os objetivos deste estudo foram avaliar a prevalência de problemas dermatológicos em pacientes com doença renal crônica (DRC em hemodiálise. Métodos: Cento e quarenta e cinco pacientes com doença renal crônica em hemodiálise foram estudados. Todos os pacientes foram completamente analisados para as alterações cutâneas, de cabelos, mucosas e unhas por um único examinador e foram coletados dados de exames laboratoriais. Os dados foram armazenados em um banco de dados do Microsolft Excel e analisados por estatística descritiva. As variáveis contínuas foram comparadas pelo teste t de Student e as variáveis categóricas utilizando o teste do qui-quadrado ou o teste Exato de Fischer, conforme adequado. Resultados: O estudo incluiu 145 pacientes, com idade média de 53,6 ± 14,7 anos, predominantemente do sexo masculino (64,1% e caucasianos (90,0%. O tempo médio de diálise foi de 43,3 ± 42,3 meses. As principais doenças subjacentes foram: hipertensão arterial em 33,8%, diabetes mellitus em 29,6% e glomerulonefrite crônica em 13,1%. As principais manifestações dermatológicas observadas foram: xerose em 109 (75,2%, equimose em 87 (60,0%, prurido em 78 (53,8% e lentigo em 33 (22,8% pacientes. Conclusão: O nosso estudo mostrou a presença de mais do que uma dermatose por paciente. As alterações cutâneas são frequentes em pacientes em diálise. Mais estudos são necessários para melhor caracterização e manejo destas dermatoses.

  4. Cytotoxicity and Glycan-Binding Properties of an 18 kDa Lectin Isolated from the Marine Sponge <em>Halichondria em>okadai>

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    Yasuhiro Ozeki

    2012-04-01

    Full Text Available A divalent cation-independent lectin—HOL-18, with cytotoxic activity against leukemia cells, was purified from a demosponge, <em>Halichondria okadaiem>. HOL-18 is a 72 kDa tetrameric lectin that consists of four non-covalently bonded 18 kDa subunits. Hemagglutination activity of the lectin was strongly inhibited by chitotriose (GlcNAcβ1-4GlcNAcβ1-4GlcNAc, fetuin and mucins from porcine stomach and bovine submaxillary gland. Lectin activity was stable at pH 4–12 and temperatures lower than 60 °C. Frontal affinity chromatography with 16 types of pyridylaminated oligosaccharides indicated that the lectin had an affinity for <em>N>-linked complex-type and sphingolipid-type oligosaccharides with <em>N>-acetylated hexosamines and neuramic acid at the non-reducing termini. The lectin killed Jurkat leukemia T cells and K562 erythroleukemia cells in a dose- and carbohydrate-dependent manner.

  5. Soil organisms associated to the weed suppressant Crotalaria juncea (fabaceae and its importance as a refuge for natural enemies Organismos de solo associados à supressora de plantas daninhas Crotalaria juncea (fabaceae e sua importância como refúgio para inimigos naturais

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    W.S Tavares

    2011-09-01

    Full Text Available Soil organisms play an important role in organic crops of Crotalaria juncea (Fabaceae and are associated with the natural conservation of the environment. The present study was aimed to investigate the population of soil organisms in the organic culture of C. juncea, as well as its importance as a refuge for natural enemies. Dalbulus maidis (Hemiptera: Cicadellidae, Diabrotica sp. (Coleoptera: Chrysomelidae, Doru luteipes (Dermaptera: Forficulidae, Gryllus assimilis (Orthoptera: Gryllidae, Lagria villosa (Coleoptera: Lagriidae, Melanotus sp. (Coleoptera: Elateridae, Meloidogyne incognita (Tylenchida: Heteroderidae, Nephila clavipes (Araneae: Nephilidae, Orius insidiosus (Hemiptera: Anthocoridae, Pheidole sp. (Hymenoptera: Myrmicidae, Phyllophaga sp. (Coleoptera: Scarabeidae, Procornitermes sp. (Isoptera: Termitidae, Solenopsis sp. (Hymenoptera: Formicidae, and Utetheisa ornatrix (Lepidoptera: Arctiidae were identified in C. juncea. The organisms that were found during a 3-month period in 144 trenches in C. juncea were pest species (84.47% and natural enemies (15.53% as well. Natural enemies had an average of 11.89 individuals per 1.08 m³ of soil cultivated with C. juncea. The abundance of organisms in the pod stage (5.49% of C. juncea was lower than that in the vegetative (83.50% and flowering (11.01% stages. Crotalaria juncea plants can be used as part of a crop system for Integrated Pest Management.Organismos de solo desempenham um importante papel em cultivos orgânicos de Crotalaria juncea (Fabaceae e estão associados com a conservação natural do ambiente. O presente estudo teve como objetivo investigar a população de organismos de solo no cultivo orgânico de C. juncea, bem como sua importância como um refúgio para inimigos naturais. Dalbulus maidis (Hemiptera: Cicadellidae, Diabrotica sp. (Coleoptera: Chrysomelidae, Doru luteipes (Dermaptera: Forficulidae, Gryllus assimilis (Orthoptera: Gryllidae, Lagria villosa (Coleoptera

  6. Soil macrofauna under integrated crop-livestock systems in a Brazilian Cerrado Ferralsol Macrofauna edáfica em sistemas de integração lavoura-pecuária num Latossolo Vermelho do Cerrado

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    Robélio Leandro Marchão

    2009-08-01

    morfoespécies. O total de 194 morfoespécies foi encontrado, distribuído entre 30 grandes grupos, dos quais os mais representativos em ordem decrescente de densidade foram: Isoptera, larvas de Coleoptera, Formicidae, Oligochaeta, adultos de Coleoptera, Diplopoda, Hemiptera, larvas de Diptera, Arachnida, Chilopoda, Lepidoptera, Gasteropoda, Blattodea e Orthoptera. Os sistemas de uso e de preparo do solo afetaram a estrutura das comunidades da macrofauna edáfica. Os sistemas de integração lavoura-pecuária, associados ao plantio direto na fase lavoura, especialmente com a rotação gramíneas/leguminosas, tiveram as melhores condições para o desenvolvimento das espécies de "engenheiros do solo" em comparação às pastagens e às culturas contínuas. A densidade e a diversidade da macrofauna, avaliadas em nível de morfo-espécies, são dados eficientes para medir o impacto de diferentes sistemas de uso da terra, em solos do Cerrado.

  7. Over-Expression of CYP2E1 mRNA and Protein: Implications of Xenobiotic Induced Damage in Patients with <em>De Novoem> Acute Myeloid Leukemia with inv(16(p13.1q22; <em>CBFβ>-MYH11em>

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    Carlos E. Bueso-Ramos

    2012-08-01

    Full Text Available Environmental exposure to benzene occurs through cigarette smoke, unleaded gasoline and certain types of plastic. Benzene is converted to hematotoxic metabolites by the hepatic phase-I enzyme CYP2E1, and these metabolites are detoxified by the phase-II enzyme NQO1. The genes encoding these enzymes are highly polymorphic and studies of these polymorphisms have shown different pathogenic and prognostic features in various hematological malignancies. The potential role of different cytochrome p450 metabolizing enzymes in the pathogenesis of acute myeloid leukemia (AML in an area of active interest. In this study, we demonstrate aberrant CYP2E1 mRNA over-expression by quantitative real-time polymerase chain reaction in 11 cases of <em>de novoem> AML with inv(16; CBFβ-MYH11. CYP2E1 mRNA levels correlated with <em>CBFβ>-MYH11 em>transcript levels and with bone marrow blast counts in all cases. CYP2E1 over-expression correlated positively with NQO1 mRNA levels (R2 = 0.934, n = 7. By immunohistochemistry, CYP2E1 protein was more frequently expressed in AML with inv(16 compared with other types of AML (<em>p> < 0.001. We obtained serial bone marrow samples from two patients with AML with inv(16 before and after treatment. CYP2E1 mRNA expression levels decreased in parallel with <em>CBFβ>-MYH11 em>transcript levels and blast counts following chemotherapy. In contrast, CYP1A2 transcript levels did not change in either patient. This is the first study to demonstrate concurrent over-expression of CYP2E1 and NQO1 mRNA in AML with inv(16. These findings also suggest that a balance between CYP2E1 and NQO1 may be important in the pathogenesis of AML with inv(16.

  8. Toxicidade do extrato aquoso das folhas de Anacardium humile para Bemisia tuberculata Toxicity of aqueous extract of Anacardium humile leaves on Bemisia tuberculata

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    Nézio Nery de Andrade Filho

    2010-08-01

    Full Text Available A mosca-branca Bemisia tuberculata (Bondar, 1923 (Hem.: Aleyrodidae é uma das principais pragas da cultura da mandioca no Estado do Mato Grosso do Sul; no entanto, não há produtos registrados para seu controle. Produtos vegetais podem constituir em alternativa viável de controle de insetos e, por sua fotodegradabilidade, diminuir os riscos ao ambiente e ao homem. Objetivou-se, por meio deste trabalho, avaliar o efeito do extrato aquoso do "cajuzinho-do-cerrado" Anacardium humile St Hill (Anacardeaceae, nas concentrações de 2,0; 0,8; 0,4 e 0,05%, sobre a biologia de B. tuberculata, bem como identificar a classe de metabólitos secundários presentes no referido extrato. O experimento foi conduzido em casa de vegetação, sob temperatura de 26±4°C. O extrato aquoso de A. humile causou aumento da duração da fase larval e mortalidade significativamente superior à testemunha em todas as concentrações testadas. Concluiu-se que o extrato aquoso de folhas de A. humile provoca mortalidade em ninfas de B. tuberculata e alongamento da fase das ninfas sobreviventes. O extrato aquoso de folhas de A. humile apresenta taninos, açúcares redutores e saponinas, e o seu índice de espuma (Índice Afrosimétrico é de 1250.The whitefly Bemisia tuberculata (Bondar, 1923 (Hem.: Aleyrodidae is the main pest of cassava crops in Mato Grosso do Sul state, however there are no products registered for its control. Plant products may represent a viable alternative for insect pest control and, due to their photo-degradability, they may reduce risks to the environment and to man. The aim of this study is to assess the effect of "cajuzinho-do-cerrado" Anacardium humile St Hill (Anacardeaceae aqueous leaf extract on the biology of B. tuberculata at the concentrations 2.0; 0.8; 0.4; 0.05%, as well as to identify the class of secondary metabolites present in the referred extract. The experiment was carried out in a greenhouse at a temperature of 26±4°C. The

  9. Milkweed (Gentianales: Apocynaceae): a farmscape resource for increasing parasitism of stink bugs (Hemiptera: Pentatomidae) and providing nectar to insect pollinators and monarch butterflies.

    Science.gov (United States)

    Tillman, P G; Carpenter, J E

    2014-04-01

    In peanut-cotton farmscapes in Georgia, the stink bugs Nezara viridula (L.) and Chinavia hilaris (Say) (Hemiptera: Pentatomidae) and the leaffooted bug, Leptoglossus phyllopus (L.) (Hemiptera: Coreidae), disperse at crop-to-crop interfaces to feed on bolls in cotton. The main objective of this study was to determine whether insecticide-free tropical milkweed (Asclepias curassavica L.), a nectar-producing plant, can increase parasitism of these bugs by Trichopoda pennipes (F.) (Diptera: Tachinidae) and provide nectar to monarch butterflies and insect pollinators in these farmscapes. Peanut-cotton plots with and without flowering milkweed plants were established in 2009 and 2010. Adult T. pennipes, monarch butterflies, honey bees, and native insect pollinators readily fed on floral nectar of milkweed. Monarch larvae feeding on milkweed vegetation successfully developed into pupae. In 2009, N. viridula was the primary host of T. pennipes in cotton, and parasitism of this pest by the parasitoid was significantly higher in milkweed cotton (61.6%) than in control cotton (13.3%). In 2010, parasitism of N. viridula, C. hilaris, and L. phyllopus by T. pennipes was significantly higher in milkweed cotton (24.0%) than in control cotton (1.1%). For both years of the study, these treatment differences were not owing to a response by the parasitoid to differences in host density, because density of hosts was not significantly different between treatments. In conclusion, incorporation of milkweed in peanut-cotton plots increased stink bug parasitism in cotton and provided nectar to insect pollinators and monarch butterflies.

  10. Hallazgo de Triatoma platensis Neiva, 1913 (Hemiptera, Triatominae en el estado brasileño de "Rio Grande do Sul" Finding of Triatoma platensis Neiva, 1913 (Hemiptera, Triatominae in the Brazilian State of "Rio Grande do Sul"

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    Roberto Salvatella

    1991-02-01

    Full Text Available Triatoma platensis Neiva 1913 (Hemiptera, Triatominae, especie ornitófila, con área de dispersión conocida en Argentina, Bolivia, Paraguay y Uruguay es notificada en un primer registro para Brasil. Hallada en el municipio de Uruguaiana (Río Grande do Sul en nidos de Anumbius annumbi (Vieillot, 1817 (Passeriformes, Furnariidae donde cohabitaba con cricétidos de la especie Orizomys flavescens. Ningún ejemplar de los siete colectados se presentó infectado por Trypanosoma cruzi.Triatoma platensis, an ornitophilic species, found in Argentina, Bolivia, Paraguay and Uruguay is reported for the first time in Brazil. It was found in the county of Uruguaiana (Rio Grande do Sul in nests of Anumbius annumbi (Vieillot, 1817 (Passeriformes, Furnariidae where it cohabited with cricetids belonging to the species Orizomys flavescens. None of the seven individuals collected were infected by Trypanosoma cruzi.

  11. Unaspis lansivora sp. n. (Hemiptera: Diaspididae), a new pest of Lansium domesticum (Meliaceae), and a key to Unaspis species.

    Science.gov (United States)

    Watson, Gillian W

    2015-01-13

    Since 2004, an undescribed species of Unaspis (Hemiptera: Diaspididae) has become a damaging pest on Lansium domesticum Corrêa in the Philippines. Its attack on the leaves causes premature senescence and defoliation, resulting in the production of few, underdeveloped, sour fruit and sometimes killing the trees. The scale was misidentified initially as Lepidosaphes ulmi (Linnaeus) and then as Unaspis citri (Comstock), but further study indicated that it was an undescribed species of potential plant quarantine significance. The pest is described as U. lansivora sp. n. and an identification key to all 19 species of Unaspis is provided. Its distribution, host range and prospects for its biological control are discussed.

  12. A new species of the genus Mahanarva Distant, 1909 (Hemiptera: Cercopoidea: Cercopidae, with a key to the species from Central America and Mexico

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    Ulises Castro–Valderrama

    2018-02-01

    Full Text Available The genus Mahanarva Distant, 1909 (Hemiptera: Cercopoidea: Cercopidae currently includes two subgenera: Mahanarva Distant, 1909 with 38 species and six subspecies, and Ipiranga Fennah, 1968 with nine species. The Manaharva species are all from the Americas, and a few species are important pests in pasture grasses and sugarcane. There are no reports of any Manaharva species from North America, including Mexico and areas to the north. Here, a new species is described from Mexico and a key to the species of Mahanarva from Central America and Mexico is proposed.

  13. Metodologia para produção de Metarhizium anisopliae (METSCH. sorokin em cultivo submerso: esporulação da biomassa, efeito da concentração de açúcar e custo do inoculant Methodology for production of Metarhizium anisopliae (METSCH. sorokin in submerged cultivation: biomass sporulation, sugar concentration effect and inoculant cost

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    Sonia Regina de Mello Pereira

    1999-09-01

    Full Text Available Desenvolveram-se um método de cultivo e um meio de cultura para produção massal do fungo Metarhizium anisopliae (Metsch. Sorokin, 1883, com maior pureza e concentração de conídios. Este método envolveu o cultivo submerso da linhagem M-61 do entomopatógeno em meio líquido de arroz parboilizado, extrato de levedura, extrato do percevejo da soja (Nezara viridula (L., 1758 Hemiptera, Pentatomidae, sob seis diferentes níveis de concentração de açúcar (0, 2, 4, 6, 8, 10g l-1, além do meio convencional sólido de arroz em grão. As biomassas obtidas foram separadas através de tela de nylon (63 mesh e dispostas em estufa para a esporulação. Os efeitos dos tratamentos foram avaliados pelos parâmetros pesos fresco e seco do micélio, número de conídios por grama de substrato, viabilidade e patogenicidade dos conídios sobre o percevejo. Observou-se que 2.0g l-1 de açúcar em meio de cultura de extrato de N. viridula produziu o dobro do número de conídios por grama de substrato em relação à concentração de 10.0g l-1, a um custo 51 vezes inferior ao obtido no processo convencional de produção do fungo. A viabilidade não foi afetada nos diferentes meios utilizados. Não ocorreram diferenças significativas na patogenicidade em função dos meios de cultura e métodos de cultivo.A method of cultivation and a culture medium were developed aiming at the mass production of fungus Metarhizium anisopliae (Metsch. Sorokin, 1883, with great concentration and purity of conidia. This method involved the M-61 strain of entomopathogenic fungus in liquid medium of rice, yeast extract, soybean bug extract (Nezara viridula (L., 1758 Hemiptera, Pentatomidae, under six differents concentrations of sugar (0, 2, 4, 6, 8, 10g l-1, and the solid conventional medium of rice grains. The biomasses obtained were filtered and put in an incubator to promote sporulation. The treatments were evaluated through the parameters wet and dry-weight of micelium

  14. First report of Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae in Brazil

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    A. L. Marsaro Júnior

    Full Text Available Abstract Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Coccoidea: Lecanodiaspididae and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae are reported for the first time in Brazil. Specimens of this scale insect were collected on branches and stems of Acacia mangium Willd., Leucaena leucocephala (Lam. de Wit (Fabaceae, Morus nigra L. (Moraceae, Citrus reticulata Blanco (Rutaceae, Tectona grandis L. f. (Verbenaceae, Anacardium occidentale (Anacardiaceae, Annona squamosa L. and Xylopia aromatica (Lam. Mart. (Annonaceae, in three municipalities of the Roraima state. All plants here mentioned are recorded for the first time as a host for L. dendrobii. Morphological characters of L. dendrobii and symptoms presented by the host plants infested by this pest are included in this work.

  15. First report of Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Lecanodiaspididae) and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae) in Brazil.

    Science.gov (United States)

    Marsaro Júnior, A L; Peronti, A L B G; Costa, V A; Morais, E G F; Pereira, P R V S

    2016-02-01

    Lecanodiaspis dendrobii Douglas, 1892 (Hemiptera: Coccoidea: Lecanodiaspididae) and the associated parasitoid Cephaleta sp. (Hymenoptera: Pteromalidae) are reported for the first time in Brazil. Specimens of this scale insect were collected on branches and stems of Acacia mangium Willd., Leucaena leucocephala (Lam.) de Wit (Fabaceae), Morus nigra L. (Moraceae), Citrus reticulata Blanco (Rutaceae), Tectona grandis L. f. (Verbenaceae), Anacardium occidentale (Anacardiaceae), Annona squamosa L. and Xylopia aromatica (Lam.) Mart. (Annonaceae), in three municipalities of the Roraima state. All plants here mentioned are recorded for the first time as a host for L. dendrobii. Morphological characters of L. dendrobii and symptoms presented by the host plants infested by this pest are included in this work.

  16. Surtos populacionais de Bemisia tabaci no estado de São Paulo Outbreaks of Bemisia tabaci in the São Paulo State, Brazil

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    André Luiz Lourenção

    1994-01-01

    Full Text Available A partir de 1991, tem sido observada a presença da mosca-branca Bemisia tabaci (Genn. (Homoptera: Aleyrodidae em altas populações em hortaliças e orna-mentais nos municípios paulistas de Paulínia, Holambra, Jaguariúna e Artur Nogueira. Foram constatadas infestações severas em tomateiro, brócolos, berinjela e aboboreira; nesta última, o sintoma observado em plantas infestadas pela mosca-branca é o prateamento da face superior das folhas, em conjunto com queda drástica da produção. Uma lavoura de tomate severamente infestada por B. tabaci apresentava o sintoma referido colo amadurecimento irregular dos frutos do tomateiro; plantas invasoras presentes nessa área também foram intensamente colonizadas, principalmente Sida rhombifolia L., Sonchus oleraceus L., Solanum viarum Dun. e Ipomoea acuminata Roem. & Schult. Em Holambra, verificaram-se ataques intensos em plantas ornamentais, principalmente crisântemo (Chrysantemum morifolium Ramat. e bico-de-papagaio (Euphorbia pulcherrima Willd.; roseiras foral pouco colonizadas. Nessas hortaliças e nas ornamentais, a aplicação quase diária de inseticidas não reduziu a infestação do inseto. Além dessas plantas, campos de algodão, em Holambra, e de feijão, em Paulínia, também foram infestados por B. tabaci. Nos E.U.A., a capacidade de certas populações de B. tabaci de induzir o prateamento da folha em aboboreira e de colonizar intensamente E. pulcherrima, entre outros fatores, têm levado à distinção do biótipo "B" ou "poinsétia", nome vulgar dessa euforbiácea; todavia, estudos recentes na Califórnia (E.U.A. mostram a possibilidade de se tratar de duas espécies distintas. Dada a similaridade entre as infestações associadas a B. tabaci que vêm ocorrendo naquele país e, mais recentemente, no Brasil, é provável que o biótipo B ou essa nova espécie tenha sido aqui introduzido.Since 1991, an increase in the populations of the whitefly Bemisia tabaci (Genn. (Homoptera

  17. Fertility life table of Lysiphlebus testaceipes (Cresson) (Hymenoptera, Braconidae, Aphidiinae) in Rhopalosiphum maidis (Fitch) and Aphis gossypii Glover (Hemiptera, Aphididae); Tabela de vida de fertilidade de Lysiphlebus testaceipes (Cresson) (Hymenoptera, Braconidae, Aphidiinae) em Rhopalosiphum maidis (Fitch) e Aphis gossypii Glover (Hemiptera, Aphididae)

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    Silva, Robson Jose da; Bueno, Vanda Helena Paes; Silva, Diego Bastos [Universidade Federal de Lavras (UFLA), MG (Brazil). Dept. de Entomologia. Lab. de Controle Biologico], e-mail: ecosbio@yahoo.com.br, e-mail: vhpbueno@ufla.br; Sampaio, Marcus Vinicius [Universidade Federal de Uberlandia, Umuarama, MG (Brazil). Inst. de Ciencias Agrarias], e-mail: mvsampaio@iciag.ufu.br

    2008-07-01

    Fertility life table of Lysiphlebus testaceipes (Cresson) (Hymenoptera, Braconidae, Aphidiinae) in Rhopalosiphum maidis (Fitch) and Aphis gossypii Glover (Hemiptera, Aphididae). The evaluation of the growth potential of Lysiphlebus testaceipes (Cresson) is important for its use in biological control programs of aphids. This work aimed to evaluate the fertility life table of L. testaceipes in Rhopalosiphum maidis (Fitch) and Aphis gossypii Glover. To determine the immature mortality, development and the sex ratio of the parasitoid, 12 females parasitoid, and 480 nymphs of each aphids were used. To evaluate the longevity and fertility 15 female parasitoid were used. Nymphs of each aphid (3 day old) were offered for each parasitoid female daily, until the female died, being 300 (first day); 250 (second day); 200 (third day); 150 (fourth day) and 50 nymphs in the other days. L. testaceipes showed immature mortality rates of 5.6 % in R. maidis and 9.2 % in A. gossypii. The development time of L. testaceipes in R. maidis and A. gossypii was 10.2 and 10.1 days, and the sex ratio of 0.71 and 0.66, respectively. The female of L. testaceipes had a fecundity of 498.8 eggs in R. maidis and 327.8 eggs in A. gossypii. The growth parameters the L. testaceipes in R. maidis and A. gossypii were, respectively R{sub o}= 205.38 and 164.08 females; r{sub m}= 0.449 and 0.431 females/females/day; {lambda}= 1.57 and 1.54 females/day; T= 11.86 and 11.83 days and TD= 10.78 and 11.27 days. L. testaceipes showed great growth potential on both aphid hosts. R. maidis could be a suitable host for proposals of mass-rearing and open rearing system using L. testaceipes. (author)

  18. Effects Of Irradiation On Mealybug Dysmicoccus neobrevipes (Hemiptera: Pseudococcidae) Infesting On Dragon Fruits

    International Nuclear Information System (INIS)

    Doan Thi The; Nguyen Thuy Khanh; Vo Kim Lang; Cao Van Chung; Tran Thien An; Nguyen Hoang Hanh Thi

    2011-01-01

    Since 2008 APHIS/USDA approved to import dragon fruit into the United States from Vietnam if fruits are treated with the minimum absorbed dose of 400 Gy to control fruit flies and mealybugs. However, a lower dose could be accepted if released data is enough to demonstrate that it is efficient to inhibit the development and stop the reproduction of adult females for three species of mealybugs (Order: Hemiptera; Family: Pseudococcidae) infesting on dragon fruit. Four development stages including 1 st , 2 nd , 3 rd instars and adult of Dymicoccus neobrevipes by isolating and rearing in the laboratory conditions were irradiated in Co-60 source gamma with dosages of 100, 200, 300 and 400 Gy. The main purposes of the study are to find the most tolerant stage and to determine the most optimal dose to control D. neobrevipes. The initial obtained results showed that the adult stage is the most radiotolerant and the dose of 200 Gy could be an effective dose to inhibit development and to control reproduction of D. neobrevipes. (author)

  19. Effects of gamma irradiation on different stages of mealybug Dysmicoccus neobrevipes (Hemiptera: Pseudococcidae)

    Science.gov (United States)

    The, Doan Thi; Khanh, Nguyen Thuy; Lang, Vo Thi Kim; Van Chung, Cao; An, Tran Thi Thien; Thi, Nguyen Hoang Hanh

    2012-01-01

    Utilization of phytosanitary irradiation as a potential treatment to disinfest agricultural commodities in trade has expanded rapidly in the recent years. Cobalt-60 gamma ray target doses of 100, 150, 200 and 250 Gy were used to irradiate immatures and adults of Dysmicoccus neobrevipes (Beardsley) (Hemiptera: Pseudococcidae) infesting dragon fruits to find the most tolerant stage and the most optimal dose range for quarantine treatment. In general, irradiation affected significantly all life stages of D. neobrevipes mortality and adult reproduction. The pattern of tolerance to irradiation in D. neobrevipes was 1st instars<2nd instars<3rd instars

  20. The foraging behavior of Diaeretiella rapae (Hymenoptera: Braconidae on Diuraphis noxia (Hemiptera: Aphididae

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    Tazerouni Zahra

    2011-01-01

    Full Text Available Host stage preference, functional response and mutual interference of Diaeretiella rapae (McIntosh (Hymenoptera: Braconidae: Aphidiinae on Diuraphis noxia (Mordvilko (Hemiptera: Aphididae were investigated under defined laboratory conditions (20±1°C; 60±5% relative humidity; 16 h light/8 h dark photoperiod. Nicholson’s model and linear regression were used to determine per capita search-efficiency and the interference coefficient, respectively. There was a significant difference between the rates of parasitism on different stages of D. noxia. The highest parasitism percentage was observed on the third instar nymphs of D. noxia in both choice and no-choice preference tests. Results of logistic regression revealed a type II functional response. The estimated values of search-efficiency (a and handling time (Th were 0.072 h-1 and 0.723 h, respectively. The maximum attack rate was calculated to be 33.22. The per capita search-efficiency decreased from 0.011 to 0.004 (h-1 as parasitoid densities increased from 1 to 8. Therefore, different host-parasitoid ratios can affect the efficacy of D. rapae.

  1. Dose-response relationships of clothianidin, imidacloprid, and thiamethoxam to Blissus occiduus (Hemiptera: Blissidae).

    Science.gov (United States)

    Stamm, M D; Baxendale, F P; Heng-Moss, T M; Siegfried, B D; Blankenship, E E; Gaussoin, R E

    2011-02-01

    The western chinch bug, Blissus occiduus Barber (Hemiptera: Blissidae), has emerged as a serious pest of buffalograss, Buchlod dactyloides (Nuttall) Engelmann. In general, neonicotinoid insecticides effectively control a variety of turfgrass insects, particularly phloem-feeding pests. However, because of well documented inconsistencies in control, these compounds are generally not recommended for chinch bugs. This study was designed to document the contact and systemic toxicity of three neonicotinoid insecticides (clothianidin, imidacloprid, and thiamethoxam) to B. occiduus. In contact bioassays, thiamethoxam was approximately 20-fold less toxic than clothianidin or imidacloprid to B. occiduus nymphs and three-fold more toxic to adults. In adult systemic bioassays, thiamethoxam was up to five-fold more toxic than clothianidin or imidacloprid. Interestingly, thiamethoxam was significantly more toxic to adults than to nymphs in both contact and systemic bioassays. This was not observed with clothianidin or imidacloprid. Bifenthrin, used for comparative purposes, exhibited 1844-fold and 122-fold increase in toxicity to nymphs and adults, respectively. These results provide the first documentation of the relative toxicity of these neonicotinoid insecticides to B. occiduus.

  2. Morphometric comparisons of Diaphorina citri (Hemiptera: Liviidae) populations from Iran, USA and Pakistan.

    Science.gov (United States)

    Lashkari, Mohammadreza; Hentz, Matthew G; Boykin, Laura M

    2015-01-01

    The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), vector of citrus greening disease pathogen, Huanglongbing (HLB), is considered the most serious pest of citrus in the world. Prior molecular based studies have hypothesized a link between the D. citri in Iran and the USA (Florida). The purpose of this study was to collect morphometric data from D. citri populations from Iran (mtCOI haplotype-1), Florida (mtCOI haplotype-1), and Pakistan (mtCOI haplotype-6), to determine whether different mtCOI haplotypes have a relationship to a specific morphometric variation. 240 samples from 6 ACP populations (Iran-Jiroft, Chabahar; Florida-Ft. Pierce, Palm Beach Gardens, Port St. Lucie; and Pakistan-Punjab) were collected for comparison. Measurements of 20 morphological characters were selected, measured and analysed using ANOVA and MANOVA. The results indicate differences among the 6 ACP populations (Wilks' lambda = 0.0376, F = 7.29, P citri species in this study, (1) Iran, (2) USA (Florida) and (3) Pakistan population. Morphometric comparisons provided further resolution to the mtCOI haplotypes and distinguished the Florida and Iranian populations.

  3. Diversidade de insetos antófilos em áreas com reflorestamento de eucalipto, município de Triunfo, Rio Grande do Sul, Brasil Diversity of anthophile insects in an area with eucalypt plantations in the municipality of Triunfo, Rio Grande do Sul, Brazil

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    Letícia Azambuja Lopes

    Full Text Available O efeito da fragmentação de habitats tem sido avaliado medindo-se a riqueza e diversidade de determinados grupos funcionais, especialmente insetos visitantes florais. Esses organismos possibilitam conectividade funcional entre plantações e áreas protegidas e a restauração de ambientes alterados. Este estudo objetivou analisar a diversidade de insetos antófilos ao longo de transectos estabelecidos entre mata ciliar e reflorestamento de eucaliptos e exclusivamente entre talhões de eucaliptos, no sul do Brasil. Os insetos coligidos pertencem às ordens Hymenoptera, Lepidoptera, Diptera, Coleoptera e Hemiptera. Comparativamente a outros estudos, a abundância de insetos foi baixa. Vinte e oito espécies foram encontradas em ambos ambientes, enquanto 138 foram exclusivas em um ambiente (61 em mata ciliar/eucaliptos e 77 em eucaliptos/eucaliptos. Os insetos visitaram flores de 31 espécies vegetais, representantes de 20 famílias. Nos dois ambientes foram encontradas poucas espécies dominantes e um grande número representadas por um único indivíduo. A alta diversidade foi similar nos dois ambientes de estudo (H' = 4,084 e H' = 4,018. Entre os insetos, as abelhas mostraram a maior diversidade nos ambientes mata ciliar/eucaliptos (H' = 3,211 e eucaliptos/eucaliptos (H' = 2,8. Considerando-se a similaridade na estrutura da comunidade entre os ambientes, a fauna de insetos antófilos difere quanto à composição de espécies e número de indivíduos das espécies amostradas.The result of habitat fragmentation has been assessed through measuring of richness and diversity of some functional groups, especially flower-visiting insects. These organisms allow functional connectivity between plantations and protected areas and the restoration of disturbed environments. This study aimed to analyze the diversity of anthophilous insects on transects established between a gallery forest and the eucalyptus reforestation and exclusively among a section

  4. First record of Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae severely damaging field grown potato crops in South Sulawesi, Indonesia

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    Nasruddin Andi

    2016-04-01

    Full Text Available Greenhouse whitefly, Trialeurodes vaporariorum Westwood was reported for the first time in the South Sulawesi Province of Indonesia, and is causing significant damage to field grown potato crops. In an insecticide trial, the tuber yield in infested, untreated plants was reduced by 39%, and the plants had an average number of 68 adult whiteflies per leaflet.

  5. Effects of Three Insect Growth Regulators on Encarsia formosa (Hymenoptera: Aphelinidae), an Endoparasitoid of Bemisia tabaci (Hemiptera: Aleyrodidae).

    Science.gov (United States)

    Wang, Q L; Liu, T-X

    2016-12-01

    Insect growth regulators (IGRs) disrupt the normal activity of the endocrine or hormone system of insects, affecting the development, reproduction, or metamorphosis of the target insects, and normally causing less detrimental effects to beneficial insects. The effects of three IGRs (pyriproxyfen, fenoxycarb, and buprofezin) on Encarsia formosa Gahan, an endoparasitoid of whiteflies, were determined using B. tabaci as a host. We assessed the effects of the IGRs on parasitoid's larval development, pupation, emergence, and contact effects of the dry residues on plant leaf and glass vial surface on adult mortality and parasitism. When the three IGRs were applied at larval stage, no or few larvae pupated in the pyriproxyfen treatments and the highest concentration of fenoxycarb, and a majority of larvae pupated in the buprofezin treatments; of those pupated, 62.3-88.1% became adults. When the IGRs were applied at the pupal stage, 2.3-17.5% developed to adults in the pyriproxyfen treatments, 59.7-89.0% in the fenoxycarb treatments, and 58.4-83.6% in the buprofezin treatments. The leaf residues of the IGRs had no appreciable effects on adults, whereas the residues on glass vial caused significantly lower adult survival than on plant leaves. The residues of pyriproxyfen and fenoxycarb slightly reduced parasitism as compared with buprofezin and controls. However, the rates of parasitoids that became adults were significantly lower, especially in the pyriproxyfen treatments. According to the standards of International Organization of Biological Control (IOBC), pyriproxyfen was harmful, while fenoxycarb and buprofezin were slightly or moderately harmful to larvae and harmless to E. formosa pupae. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  6. Whitefly species (Hemiptera: Aleyrodidae on wild and cultivated plants in the horticultural region of Rosario, Santa Fe, Argentina

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    Gustavo G. GONSEBATT

    2012-01-01

    Full Text Available Las moscas blancas de importancia económica son polífagas y capaces de desarrollarse sobre numerosas plantas cultivadas y espontáneas. Registramos las especies de moscas blancas sobre cultivos hortícolas y de flores, y sobre las plantas silvestres asociadas. Observamos dos especies: Trialeurodes vaporariorum (Westwood y el complejo Bemisia tabaci (Gennadius. T. vaporariorum fue registrada sobre 24 especies de plantas (11 familias, 12 y 8 de las cuales son hospedantes nuevos para Argentina y a nivel mundial, respectivamente. El complejo B. tabaci fue registrado solo en sistemas de producción de flores, sobre 19 especies de plantas (11 familias, 14 y 7 de las cuales son nuevos hospedantes para Argentina y a nivel mundial, respectivamente. Los cultivos Glycine max (L. y Chrysanthemum morifolium Ramat., las especies silvestres Amaranthus blitum L., Amaranthus quitensis Kunth, Conyza bonariensis (L., Galinsoga parviflora Cav., Sonchus oleraceus L. y Wedelia glauca (Ortega O. Hoffm. ex Hicken fueron hospedantes de ambas especies. El único parasitoide registrado fue Eretmocerus californicus cercano a corni Haldeman (Hymenoptera: Aphelinidae sobre T. vaporariorum. Este estudio que constituye el primer relevamiento sistemático de plantas hospedantes en la región, aporta un mayor conocimiento sobre el rango de plantas hospedantes de las moscas blancas en Argentina.

  7. Life-history parameters of Encarsia formosa, Eretmocerus eremicus and E. Mundus, aphelinid parasitoids of Bemisia argentifolii (Hemiptera: Aleyrodidae)

    NARCIS (Netherlands)

    Qiu, Y.T.; Lenteren, van J.C.; Drost, Y.C.; Posthuma-Doodeman, J.A.M.

    2004-01-01

    Life-history parameters (juvenile development time, adult longevity, host instar preference and rate of parasitism) offour parasitoids of Bemisia argentifolii (two strains of Encarsia formosa (D and B), Eretmocerus eremicus and Eretmocerus mundus) Were Studied in the laboratory. At 15degreesC

  8. Genome-Wide Characterization and Expression Profiling of Sugar Transporter Family in the Whitefly, Bemisia tabaci (Gennadius (Hemiptera: Aleyrodidae

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    Zezhong Yang

    2017-05-01

    Full Text Available Sugar transporters (STs play pivotal roles in the growth, development, and stress responses of phloem-sucking insects, such as the whitefly, Bemisia tabaci. In this study, 137 sugar transporters (STs were identified based on analysis of the genome and transcriptome of B. tabaci MEAM1. B. tabaci MEAM1 encodes a larger number of STs than other selected insects. Phylogenetic and molecular evolution analysis showed that the 137 STs formed three expanded clades and that the genes in Sternorrhyncha expanded clades had accelerated rates of evolution. B. tabaci sugar transporters (BTSTs were divided into three groups based on their expression profiles across developmental stages; however, no host-specific BTST was found in B. tabaci fed on different host plants. Feeding of B. tabaci adults with feeding diet containing dsRNA significantly reduced the transcript level of the target genes in B. tabaci and mortality was significantly improved in B. tabaci fed on dsRNA compared to the control, which indicates the sugar transporters may be used as potential RNAi targets for B. tabaci bio-control. These results provide a foundation for further studies of STs in B. tabaci.

  9. Avaliação de óleos essenciais de plantas aromáticas com atividade inseticida em Triatoma infestans (Klug, 1834 (Hemiptera: Reduviidae = Evaluation of essential oils with insecticidal activity in Triatoma infestans (Klug, 1834 (Hemiptera: Reduviidae

    Directory of Open Access Journals (Sweden)

    Suellem Petilim Gomes

    2011-07-01

    Full Text Available A doenca de Chagas e um dos maiores problemas de saude publica na America Latina. Neste trabalho, foi avaliada a acao inseticida de oleos essenciais de Anacardium humile Cymbopogon nardus, Ocimum basilicum e Eucalyptus urograndis em ninfas do 3‹ e 4‹ estadios dedesenvolvimento de Triatoma infestans por acao topica e pressao a vapor (fumigacao. Para o teste topico foram realizadas duas repeticoes cada uma com cinco ninfas de 3‹ e 4‹ estadios de desenvolvimento de T. infestans. Foi aplicado 1ƒÊL de cada concentracao na regiao dorsal de cada individuo. Para o teste de fumigacao foram utilizados potes de 2 L com tampa vedante. Os oleos essenciais de O. gratissimum e E. urograndis apresentaram atividadeinseticida para T. infestans, e A. humile e C. nardus apresentaram em media 5 e 15% de mortalidade, respectivamente. Para fumigacao, somente o oleo de E. urograndis teve efeito, com 100% de mortalidade sobre T. infestans , sendo que para os outros oleos nao foiobservado efeito de fumigacao. Esse trabalho relata pela primeira vez a acao inseticida de E. urograndis em potes fumigenos para o controle de triatomineos. Os resultados sao importantes devido a busca por novos modelos moleculares com acao inseticida em triatomineos resistentes aos inseticidas sinteticos.Chagas disease is a major public health problem in Latin America. This work aimed to evaluate the insecticide action of essential oils of Anacardium humile, Cymbopogon nardus, Ocimum basilicum and Eucalyptus urograndis on Triatoma infestans nymphs of third and fourth stages of development by topic action and steam pressure (fumigation. Two repetitions were performed at the topic test with five nymphs of third and fourth stages of development of T. infestans. It was applied 1ƒÊL of each concentration on the dorsal region of each nymph. Two-liter pots with sealing covers were used at the fumigation test. The essential oils of O. gratissimum and E. urograndis showed insecticidal

  10. Thermal Studies of Zn(II, Cd(II and Hg(II Complexes of Some <em>N-Alkyl-N>-Phenyl-Dithiocarbamates

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    Peter A. Ajibade

    2012-07-01

    Full Text Available The thermal decomposition of Zn(II, Cd(II and Hg(II complexes of <em>N-ethyl-N>-phenyl and <em>N-butyl-N>-phenyl dithiocarbamates have been studied using thermogravimetric analysis (TGA and differential scanning calorimetry (DSC. The products of the decomposition, at two different temperatures, were further characterized by scanning electron microscopy (SEM and energy-dispersive X-ray spectroscopy (EDX. The results show that while the zinc and cadmium complexes undergo decomposition to form metal sulphides, and further undergo oxidation forming metal oxides as final products, the mercury complexes gave unstable volatiles as the final product.

  11. Biology and bionomics of dysdercus koenigii f. (hemiptera: pyrrhocoridae) under laboratory conditions

    International Nuclear Information System (INIS)

    Jaleel, W.; Naqqash, M.N.

    2013-01-01

    Red Cotton Bug, Dysdercus koenigii F., (Hemiptera: Pyrrhocoridae) is an important pest of cotton in South East Asia. Studies were carried out during 2012 to find the effect of temperature on incubation period and to explore the reproductive biology and bionomics of D. koenigii under laboratory conditions. Minimum incubation period (4.70+-0.42 days) was recorded at 35 degree C while the eggs failed to hatch at 40 degree C at 70-75% relative humidity. There were five nymphal instars which completed their development in 23.42+-2.49 days. The female lived longer (20.85+-6.12 days) than the male (16.18+-6.06 days). Each female mated three times in her life and there was statistically significant difference in mating duration (days), number of eggs laid and hatching percentage in each mating time. Number of eggs and hatching percentage was significantly higher after 1st time mating followed by 2nd and 3rd time matings. Duration of pre-oviposition, oviposition and post oviposition period recorded was 7.47+-0.86, 12.43+-0.82 and 8.77+-2.41 days, respectively. The study will help in devising pest management strategy against D. koenigii. (author)

  12. Phenotypic variation and identification of Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) in China.

    Science.gov (United States)

    Zhao, Jing; Watson, Gillian W; Sun, Yang; Tan, Yongan; Xiao, Liubin; Bai, Lixin

    2014-05-23

    Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) is an invasive mealybug that seriously damages cotton and other important crops. In previous studies in China, the presence of two submedian longitudinal lines of pigmented spots on the dorsum of adult females frequently has been used to identify this species. However, the present study records the occasional absence of pigmented spots in a sample from Guangxi province, China. Specimens without pigmented spots showed all the molecular and morphological characters that separate P. solenopsis from the similar species P. solani Ferris, especially the distribution of multilocular disc pores. In different geographic populations of P. solenopsis in China, mitochondrial COI and nuclear 28SrDNA genes are very similar (99.8-100%), indicating that they are conspecific. For COI, the genetic distance between P. solenopsis and P. solani is more than 3%. A map of the distribution of P. solenopsis in China is given. To help identify both pigmented and non-pigmented P. solenopsis accurately, an identification key to the 16 species of Phenacoccus found in China is provided. The key also identifies five potentially invasive Phenacoccus species not yet established in China, in case they get introduced there.

  13. Conservazione e gestione della Lepre italica (<em>Lepus corsicanusem>

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    Francesco Riga

    2003-10-01

    Full Text Available Il recente riconoscimento dello <em>status> specifico della Lepre italica (<em>Lepus corsicanusem> e l?accertamento dell?areale distributivo rappresentano le azioni più importanti per la conservazione di un <em>taxon endemicoem> che si era creduto estinto. Nella penisola la specie presenta un areale discontinuo, il cui limite settentrionale è dato dal comune di Manciano (GR, sul versante tirrenico e da una linea che dalla provincia de L'Aquila arriva al Gargano. In Sicilia la distribuzione è relativamente continua anche in aree non protette. Dati genetici hanno permesso di confermare la presenza in Corsica. Al contrario, nell?Isola d'Elba, a seguito di estese ricerche, sono stati identificati solo esemplari di <em>L. europaeusem>. Nell?Italia peninsulare <em>L. corsicanusem> è spesso presente in simpatria con popolazioni di <em>L. europaeusem>, mentre in Sicilia la lepre europea non ha originato popolazioni stabili, nonostante l?immissione di molte migliaia di individui. La distribuzione ecologica di <em>L. corsicanusem> ed analisi ambientali specifiche, suggeriscono l?adattamento prevalente agli ambienti a clima mediterraneo, benché essa sia presente anche a quote elevate (> 1.500 m s.l.m.. Dati preliminari di abbondanza relativa hanno evidenziato una situazione diversificata tra la penisola e la Sicilia e tra aree a diverso regime di gestione; un confronto tra le aree protette ha evidenziato rispettivamente valori di 5,54 e 11,73 ind./km². La riduzione quali-quantitativa e la frammentazione dell?<em>habitat> delle lepri è un fenomeno potenzialmente pericoloso per la sopravvivenza delle popolazioni, determinando fenomeni di estinzione locale dovuti alle basse densità di popolazione, inducendo fenomeni di erosione della variabilità genetica e di riduzione della <em>fitness> degli individui. L?introduzione di <em>L. europaeusem> può costituire un importante fattore limitante sia per la possibile competizione

  14. Methyl 2-Benzamido-2-(1<em>H>-benzimidazol-1-ylmethoxyacetate

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    Alami Anouar

    2012-09-01

    Full Text Available The heterocyclic carboxylic α-aminoester methyl 2-benzamido-2-(1<em>H>-benzimidazol-1-ylmethoxyacetate is obtained by <em>O>-alkylation of methyl α-azido glycinate <em>N>-benzoylated with 1<em>H>-benzimidazol-1-ylmethanol.

  15. A new mealybug in the genus Pseudococcus Westwood (Hemiptera: Coccomorpha: Pseudococcidae) from North America, with a key to species of Pseudococcus from the New World.

    Science.gov (United States)

    Ellenrieder, Natalia Von; Watson, Gillian

    2016-04-19

    A mealybug species that feeds on Agave spp., Pseudococcus variabilis sp. n. (Hemiptera: Coccomorpha: Pseudococcidae), is described from North America. Its entry into the United States was likely via the horticultural trade on its host plants in the genus Agave (Liliales: Agavaceae). Descriptions and illustrations of the adult female and male, diagnosis from congeners in the New World, and a molecular characterization based on COI are provided, as well as a key to adult females of all Pseudococcus species recorded from the New World.

  16. A preliminary molecular phylogeny of planthoppers (Hemiptera: Fulgoroidea based on nuclear and mitochondrial DNA sequences.

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    Nan Song

    Full Text Available The planthopper superfamily Fulgoroidea (Insecta: Hemiptera is one of the most dominant groups of phytophagous insects. It comprises about 20 families, containing a total of 9000 species worldwide. Despite several recent studies, the phylogeny of Fulgoroidea is not yet satisfactorily resolved and the phylogenetic positions of several key families, especially Cixiidae, Delphacidae, Tettigometridae, Nogodinidae, Acanaloniidae and Issidae, are contentious. Here, we expand upon recent phylogenetic work using additional nuclear (18S and 28S and novel mitochondrial (16S and cytb markers. Maximum likelihood and Bayesian analyses yielded robust phylogenetic trees. In these topologies, a group containing Cixiidae and Delphacidae is recovered as the sister group to the remaining taxa. Tettigometridae is placed in a more nested position and is grouped with Caliscelidae. Sister relationships are found between Flatidae and Ricaniidae, and between Dictyopharidae and Fulgoridae. Nogodinidae and Issidae are confirmed to be non-monophyletic families. For major nodes of interest, divergence date estimates are generally older than those from the fossil record.

  17. Phytoreovirus-like sequences isolated from salivary glands of the glassy-winged sharpshooter Homolodisca vitripennis (Hemiptera: Cicadellidae)

    International Nuclear Information System (INIS)

    Katsar, C.S.; Hunter, W.B.; Sinisterra, X.H.

    2007-01-01

    The salivary glands of the Glassy-winged sharpshooter (GWSS), Homalodisca vitripennis Germar 1821, (syn. H. coagulata, Hemiptera: Cicadellidae) were collected and used to produce a cDNA library. Examination by BLASTX analyses identified 2 viral sequences, one a 610-base pair fragment and a second 839-base pair fragment, both of which had significant homology to viruses within the genus Phytoreovirus. Resequencing of the fragments confirmed sequence validities. These sequences were used for in silico protein translation and BLASTP analysis confirming the established homology. While the GWSS is the primary vector of Pierce's disease of grapes, this is the first report that GWSS may be a vector of a phytoreoviruses. Phylogenetic and homology comparisons with BLASTX, BLASTP, and PAUP analyses indicated that the viral sequences isolated from GWSS were closely related to the viruses in the Family Reoviridae, Genus Phytoreovirus, specifically Rice Dwarf Phytoreovirus (RDV). RDV is the only plant reovirus that is not limited to the phloem. Phytoreoviruses are transmitted in a propagative manner by cicadellid leafhoppers (Hemiptera: Cicadellidae), which acquire and transmit them during feeding. Phytoreoviruses have been reported from Agallian, Agalliopsis, Nephotettix, and Recilia, genera of leafhoppers, with evidence for transovarial transmission. The GWSS, although considered to feed primarily from the xylem, ingests from other plant tissues, such as the phloem and mesophyll during probing similar to other leafhoppers. The feeding behavior and wide host range of the GWSS provides an overlapping condition for these two organisms, leafhopper and virus. GWSS will feed from grasses as a transitory host, and on herbaceous and woody plants as primary hosts, which may favor the acquisition and transmission of Phytoreovirus by this leafhopper. Monitoring for an increase of Phytoreovirus spread in graminaceous crops that are in proximity to vineyards or tree crop orchards, where

  18. Development of 101 Gene-based Single Nucleotide Polymorphism Markers in Sea Cucumber, <em>Apostichopus japonicusem>>

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    Wei Lu

    2012-06-01

    Full Text Available Single nucleotide polymorphisms (SNPs are currently the marker of choice in a variety of genetic studies. Using the high resolution melting (HRM genotyping approach, 101 gene-based SNP markers were developed for <em>Apostichopus japonicusem>, a sea cucumber species with economic significance for the aquaculture industry in East Asian countries. HRM analysis revealed that all the loci showed polymorphisms when evaluated using 40 <em>A. japonicusem> individuals collected from a natural population. The minor allele frequency ranged from 0.035 to 0.489. The observed and expected heterozygosities ranged from 0.050 to 0.833 and 0.073 to 0.907, respectively. Thirteen loci were found to depart significantly from Hardy–Weinberg equilibrium (HWE after Bonferroni corrections. Significant linkage disequilibrium (LD was detected in one pair of markers. These SNP markers are expected to be useful for future quantitative trait loci (QTL analysis, and to facilitate marker-assisted selection (MAS in <em>A. japonicusem>.

  19. Activity-Guided Isolation of Antioxidant Compounds from <em>Rhizophora apiculataem>

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    Hongbin Xiao

    2012-09-01

    Full Text Available <em>Rhizophora apiculataem> (<em>R. apiculataem> contains an abundance of biologically active compounds due its special salt-tolerant living surroundings. In this study, the total phenolic content and antioxidant activities of various extract and fractions of stem of <em>R. apiculataem> were investigated. Results indicated that butanol fraction possesses the highest total phenolic content (181.84 mg/g GAE/g dry extract with strongest antioxidant abilities. Following <em>in vitroem> antioxidant activity-guided phytochemical separation procedures, lyoniresinol-3α-<em>O>-β-arabinopyranoside (1, lyoniresinol-3α-<em>O>-β-rhamnoside (2, and afzelechin-3-<em>O>-L-rhamno-pyranoside (3 were separated from the butanol fraction. These compounds showed more noticeable antioxidant activity than a BHT standard in the DPPH, ABTS and hydroxyl radical scavenging assays. HPLC analysis results showed that among different plant parts, the highest content of 13 was located in the bark (0.068%, 0.066% and 0.011%, respectively. The results imply that the <em>R. apiculataem> might be a potential source of natural antioxidants and 13 are antioxidant ingredients in <em>R. apiculataem>.

  20. Alberprosenia malheiroi Serra, Atzingen & Serra, 1987 (Hemiptera, Reduviidae: redescrição e bionomia Alberprosenia malheiroi Serra, Atzingen & Serra, 1987 (Hemiptera, Rediviidae: redescription and bionomics

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    Rodolfo U. Carcavallo

    1995-12-01

    Full Text Available Sobre Alberprosenia malheiroi n. sp. deu-se a conhecer uma diagnose resumida em 1980, porém sem valor bibliográfico. Em 1987 publicou-se a mesma diagnose anexando uma foto e alguns comentários, porém sem realizar uma descrição formal. Descreve-se para esta espécie, os adultos, os estádios imaturos, determina-se a série sintípica e apresenta-se dados bionômicos e de criação em insetário. As diferenças mais evidentes com A. goyovargasi, a única espécie que se conhecia do gênero até então, são a coloração geral negra, o espaço interocular maior que o tamanho de um olho visto dorsalmente, os tubérculos do colar com o ápice agudo e o tamanho maior, na nova espécie, quase o dobro do da primeira espécie. Os ovos são pequenos, fixados ao substrato em grupos de 3 ou 4, elipsóides, não achatados lateralmente, com o opérculo proeminente, convexo, sem estruturas evidentes. As ninfas apresentam em todos os estádios caracteres típicos do gênero e da tribo, com região anteocular menos longa que a post-ocular e característica pilosidade do tegumento que vai se acentuando a cada estádio. A. malheiroi n. sp. foi capturado em ecótopos silvestres em palmeiras em floresta no Estado do Pará, associados com morcegos ou aves. Nenhum dos exemplares estava infectado com Trypanosoma cruzi. Esses triatomíneos foram mantidos em insetários a ± 25°C e ± 60% UR, são insetos ágeis e voam com relativa facilidade. Alimentaram-se bem em pombos e morcegos e não aceitaram alimentação em ratos, camundongos ou hamsters. O período de incubação dos ovos foi em média treze dias e o tempo de evolução do período ninfal foi em média cento e trinta e dois dias.Alberprosenia malheiroi n. sp. first became known through an informal publication of a paper submitted a Congress, in 1980. The same diagnosis with a photograph was published in 1987, but no formal description has yeet been made. The present authors describe adults and immature

  1. Synthesis and Spectroscopic Analysis of Novel 1<em>H-Benzo[d>]imidazoles Phenyl Sulfonylpiperazines

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    Amjad M. Qandil

    2012-05-01

    Full Text Available A group of benzimidazole analogs of sildenafil, 3-benzimidazolyl-4-methoxy-phenylsulfonylpiperazines 2–4 and 3-benzimidazolyl-4-methoxy-<em>N,N>-dimethyl- benzenesulfonamide (5, were efficiently synthesized. Compounds 2–5 were characterized by NMR and MS and contrary to the reported mass spectra of sildenafil, the spectra of the piperazine-containing compounds 2–4 showed a novel fragmentation pattern leading to an <em>m/z> = 316. A mechanism for the formation of this fragment was proposed.

  2. Osservazioni in cattività sul ciclo stagionale del peso corporeo e sull'efficienza digestiva di <em>Pipistrellus kuhliiem> e <em>Hypsugo saviiem> (Chiroptera: Verspertilionidae

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    Gianna Dondini

    2004-06-01

    Full Text Available Abstract Captivity observation on body weight cycle and digestive efficiency in <em>Pipistrellus kuhliiem> and <em>Hypsugo saviiem> (Chiroptera: Vespertilionidae Many bat species of cold-temperate climate are subject to seasonal variation of temperature and food availability. Fat reserve during summer-autumn is therefore a physiological adaptation to spend the winter months by hibernating or to sustain migration. During a research on bats in urban areas, two juveniles of Kuhl's bat (<em>Pipistrellus kuhliiem>, 2 females and two juveniles of Savi's bat (<em>Hypsugo saviiem>, 1 male and 1 female were collected in 1997 in the urban area of Florence (central Italy. Bats were kept in a cage of 50x40x30 cm with a temperature between 17° and 22° C. Every day they were weighted with an electronic balance before eating mealworms (<em>Tenebrio molitorem>. Digestive efficiency, calculated on dry material, was about 90% for both species. In about six months <em>P. kuhliiem> and <em>H. saviiem> increased on the average of 450% and 280% in weight respectively. Deposition of fat reserve seemed to be faster in <em>P. kuhliiem> than in <em>H. saviiem>. Both species showed a circannual cycle in the variation of weight. Riassunto Molte specie di pipistrelli dei climi temperato-freddi sono soggette a marcate variazioni stagionali di temperatura e disponibilità di cibo. L'accumulo di grasso in tarda estate-autunno è quindi un adattamento fisiologico per trascorrere in ibernazione i mesi invernali o per intraprendere la migrazione. Nell'ambito di una ricerca pluriennale sui pipistrelli in ambienti urbani, 4 esemplari giovani, di cui 2 di Pipistrello albolimbato (<em>Pipistrellus kuhliiem>, 2 femmine e due di Pipistrello di Savi (<em>Hypsugo saviiem>, 1 maschio e 1 femmina, sono stati raccolti nella pianura di Firenze durante l'estate del 1997 e mantenuti in un contenitore di 50x40x30 cm ad

  3. DAMPAK APLIKASI INSEKTISIDA PERMETRIN TERHADAP HAMA PENGISAP BIJAIJ HELOPELTIS SPP. (HEMIPTERA: MIRIDAE DAN ARTROPODA NON-TARGET PADA PERTANAMAN KAKAO (THEOBROMA CACAO L.

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    Rosma Hasibuan

    2014-08-01

    Full Text Available Impact of Permethrin-Insecticide Application on Mirid Pest of Cocoa Helopeltis spp. (Hemiptera: Miridae and on Non-target  Arthropods in Cocoa Plantations (Theobroma cacao L.. Cocoa mirid bugs, Helopeltis spp., (Hemiptera: Miridae are the most important pest of cocoa in Indonesia. A field study was conducted to investigate the effect of permethrin on cocoa mirids and non-target arthropods at a cocoa plantation in specific place. A randomized complete block design was used in which each of four blocks consisted of 5 treatments (4 concentrations of permethrin; 50; 100; 200; and 250 ppm and control. The results indicated that the application of permethrin significantly increased the cocoa mirids mortaliiy throughout all sprayed cocoa trees (up to 100% 72 h after application. Even at 1 h after application, the percentage of Cocoa mirids mortality 29.2% - 53.9% on cocoa trees sprayed with permethrin at concentrations of 50 - 250 ppm was significantly higher than that on control plant (3.6%. At 72 h after treatments, application of permethrin at concentrations of 200 and 250 ppm caused a complete kill (mortalty of 100% in the test mirids. Meanwhile, ground cloths caught at least 22 fanilies of abore-ground arthropods that were found killed by permethrin applications. Moreover, the number of non-target arthropods killed by permethrin at concentrations of 100 - 250 ppm (27.3 - 85.3 individuals/ground cloth were signifcantly higher than that on control trees. These results demonstrated that despite high efficacy of permethrin in controlling of cocoa mirids (Hetopeltis spp., its application also had adverse effects on non-target arthropods incocoa plantations.

  4. Determinación de la capacidad de transmisión de fitoplasmas en dos morfoespecies de la familia Cicadellidae (Hemiptera: Auchenorryncha) de Bogotá D.C.

    OpenAIRE

    Perilla Henao, Laura Margarita

    2013-01-01

    Los fitoplasmas son bacterias fitopatógenas que carecen de pared celular, especializadas en la colonización intracelular del floema de plantas, son transmitidos por insectos vectores del suborden Auchenorryncha (Hemiptera) y están entre los seres vivos con menor tamaño. Los fitoplasmas forman un grupo monofilético en la clase Mollicutes que causan alteraciones morfológicas y fisiológicas en plantas ornamentales, hortícolas, maderables, frutales y cultivos en todo el mundo. No existen métodos...

  5. REGISTRO DA COCHONILHA-ROSADA-DO-HIBISCO INFESTANDO FRUTÍFERAS EM MACEIÓ, ALAGOAS, BRASIL

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    SÔNIA MARIA FORTI BROGLIO

    2015-01-01

    Full Text Available This study reported for the first time the occurrence of the pink hibiscus mealybug Maconelli-coccus hirsutus (Green, 1908 (Hemiptera: Pseudococcidae in Maceió, Alagoas, Brazil attacking the following fruit species: soursop (Annona muricata L. (Annonaceae, guava (Psidium guajava L. (Myrtaceae, mango (Mangifera indica L. (Anacardiaceae, carambola (Averrhoa carambola L. (Oxalidaceae, acerola (Malpighia glaba L. (Malpighiaceae, cajazeiro (Spondias lutea L. (Anacardiaceae and cupuaçuzeiro (Theobroma gran-diflorum (Willd. ex Spreng. Schum. (Malvaceae. The percentages of injured plants were recorded for each plant species in the field. Damaged plants showed short internodes, wrinkled leaves, fruit deformation, and sooty mold developed on the honeydew excreted by the mealybugs. Infested plant structures were taken to the Laboratory of Entomology of the Center for Agricultural Sciences, of the Federal University of Alagoas, where the mealybug species was identified through morphological characteristics. The following injury percentages were recorded on the plants: soursop (100%, guava (10%, mango (10%, carambola (100%, acerola (100%, cajá (50% and cupuaçu (100%. The mealybug collected in Maceió, AL; Brazil was identified as Maconelli-coccus hirsutus (Green, 1908 (Hemiptera: Pseudococcidae. It was the first record of this species in the state of Alagoas and infestation by pink hibiscus mealybug on the host plants mango, acerola and cajazeiro in Brazil.

  6. Whitefly species (Hemiptera: Aleyrodidae on wild and cultivated plants in the horticultural region of Rosario, Santa Fe, Argentina Especies de moscas blancas (Hemiptera: Aleyrodidae sobre plantas silvestres y cultivadas en la región hortícola de Rosario, Santa Fe, Argentina

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    Gustavo G. Gonsebatt

    2012-06-01

    Full Text Available Whiteflies of economic importance are polyphagous, being able to develop on a large number of cultivated and spontaneous plants. We recorded the whitefly species on vegetable and flower crops and the wild plants associated, under greenhouse and field conditions, for two years. We observed two species: Trialeurodes vaporariorum (Westwood and the Bemisia tabaci complex (Gennadius. T vaporariorum was recorded on 24 plant species (11 families, 12 and 8 of which are new hosts in Argentina and in the world, respectively. The B. tabaci complex was recorded only on flower production systems, on 19 plant species (11 families, 14 and 7 of which are new hosts in Argentina and in the world, respectively. The crops Glycine max (L. and Chrysanthemum morifolium Ramat., the wild species Amaranthus blitum L., Amaranthus quitensis Kunth, Conyza bonariensis (L., Galinsoga parviflora Cav., Sonchus oleraceus L. and Wedelia glauca (Ortega O. Hoffm. ex Hicken were hosts of both species. The only parasitoid recorded was Eretmocerus californicus near corni Haldeman (Hymenoptera: Aphelinidae from T. vaporariorum. This study, which is the first systematic survey of host plants in the region, intends to provide a better knowledge of the range of whiteflies host plants in Argentina.Las moscas blancas de importancia económica son polífagas y capaces de desarrollarse sobre numerosas plantas cultivadas y espontáneas. Registramos las especies de moscas blancas sobre cultivos hortícolas y de flores, y sobre las plantas silvestres asociadas. Observamos dos especies: Trialeurodes vaporariorum (Westwood y el complejo Bemisia tabaci (Gennadius. T. vaporariorum fue registrada sobre 24 especies de plantas (11 familias, 12 y 8 de las cuales son hospedantes nuevos para Argentina y a nivel mundial, respectivamente. El complejo B. tabaci fue registrado solo en sistemas de producción de flores, sobre 19 especies de plantas (11 familias, 14 y 7 de las cuales son nuevos hospedantes para Argentina y a nivel mundial, respectivamente. Los cultivos Glycine max (L. y Chrysanthemum morifolium Ramat., las especies silvestres Amaranthus blitum L., Amaranthus quitensis Kunth, Conyza bonariensis (L., Galinsoga parviflora Cav, Sonchus oleraceus L. y Wedelia glauca (Ortega O. Hoffm. ex Hicken fueron hospedantes de ambas especies. El único parasitoide registrado fue Eretmocerus californicus cercano a corni Haldeman (Hymenoptera: Aphelinidae sobre T. vaporariorum. Este estudio que constituye el primer relevamiento sistemático de plantas hospedantes en la región, aporta un mayor conocimiento sobre el rango de plantas hospedantes de las moscas blancas en Argentina.

  7. Weed hosts of cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Vennila, S; Prasad, Y G; Prabhakar, M; Agarwal, Meenu; Sreedevi, G; Bambawale, O M

    2013-03-01

    The exotic cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) invaded India during 2006, and caused widespread infestation across all nine cotton growing states. P. solenopsis also infested weeds that aided its faster spread and increased severity across cotton fields. Two year survey carried out to document host plants of P. solenopsis between 2008 and 2010 revealed 27, 83, 59 and 108 weeds belonging to 8, 18, 10 and 32 families serving as alternate hosts at North, Central, South and All India cotton growing zones, respectively. Plant species of four families viz., Asteraceae, Amaranthaceae, Malvaceae and Lamiaceae constituted almost 50% of the weed hosts. While 39 weed species supported P. solenopsis multiplication during the cotton season, 37 were hosts during off season. Higher number of weeds as off season hosts (17) outnumbering cotton season (13) at Central over other zones indicated the strong carryover of the pest aided by weeds between two cotton seasons. Six, two and seven weed hosts had the extreme severity of Grade 4 during cotton, off and cotton + off seasons, respectively. Higher number of weed hosts of P. solenopsis were located at roadside: South (12) > Central (8) > North (3) zones. Commonality of weed hosts was higher between C+S zones, while no weed host was common between N+S zones. Paper furnishes the wide range of weed hosts of P. solenopsis, discusses their significance, and formulated general and specific cultural management strategies for nationwide implementation to prevent its outbreaks.

  8. How will Mahanarva spectabilis (Hemiptera: Cercopidae) Respond to Global Warming?

    Science.gov (United States)

    Fonseca, M G; Auad, A M; Resende, T T; Hott, M C; Borges, C A V

    2016-01-01

    The aim of this study was to determine the favorable constant temperature range for Mahanarva spectabilis(Distant) (Hemiptera: Cercopidae) development as well as to generate geographic distribution maps of this insect pest for future climate scenarios. M. spectabilis eggs were reared on two host plants (Brachiaria ruziziensis(Germain and Edvard) and Pennisetum purpureum(Schumach)), with individual plants kept at temperatures of 16, 20, 24, 28, and 32 °C. Nymphal stage duration, nymphal survival, adult longevity, and egg production were recorded for each temperature*host plant combination. Using the favorable temperature ranges for M. spectabilis development, it was possible to generate geographic distribution. Nymphal survival was highest at 24.4 °C, with estimates of 44 and 8% on Pennisetum and Brachiaria, respectively. Nymphal stage duration was greater on Brachiaria than on Pennisetum at 20 and 24 °C but equal at 28 °C. Egg production was higher on Pennisetum at 24 and 28 °C than at 20 °C, and adult longevity on Pennisetum was higher at 28 °C than at 20 °C, whereas adult longevity at 24 °C did not differ from that at 20 and 28 °C. With these results, it was possible to predict a reduction in M. spectabilis densities in most regions of Brazil in future climate scenarios. © The Author 2016. Published by Oxford University Press on behalf of the Entomological Society of America.

  9. New views on strand asymmetry in insect mitochondrial genomes.

    Directory of Open Access Journals (Sweden)

    Shu-Jun Wei

    Full Text Available Strand asymmetry in nucleotide composition is a remarkable feature of animal mitochondrial genomes. Understanding the mutation processes that shape strand asymmetry is essential for comprehensive knowledge of genome evolution, demographical population history and accurate phylogenetic inference. Previous studies found that the relative contributions of different substitution types to strand asymmetry are associated with replication alone or both replication and transcription. However, the relative contributions of replication and transcription to strand asymmetry remain unclear. Here we conducted a broad survey of strand asymmetry across 120 insect mitochondrial genomes, with special reference to the correlation between the signs of skew values and replication orientation/gene direction. The results show that the sign of GC skew on entire mitochondrial genomes is reversed in all species of three distantly related families of insects, Philopteridae (Phthiraptera, Aleyrodidae (Hemiptera and Braconidae (Hymenoptera; the replication-related elements in the A+T-rich regions of these species are inverted, confirming that reversal of strand asymmetry (GC skew was caused by inversion of replication origin; and finally, the sign of GC skew value is associated with replication orientation but not with gene direction, while that of AT skew value varies with gene direction, replication and codon positions used in analyses. These findings show that deaminations during replication and other mutations contribute more than selection on amino acid sequences to strand compositions of G and C, and that the replication process has a stronger affect on A and T content than does transcription. Our results may contribute to genome-wide studies of replication and transcription mechanisms.

  10. Ecotope effect in Triatoma brasiliensis (Hemiptera: Reduviidae) suggests phenotypic plasticity rather than adaptation.

    Science.gov (United States)

    Batista, V S P; Fernandes, F A; Cordeiro-Estrela, P; Sarquis, O; Lima, M M

    2013-09-01

    Triatoma brasiliensis (Hemiptera: Reduviidae) is an important vector of Chagas' disease in both sylvatic and peridomestic ecotopes. Discriminating between these populations of Triatominae has been proposed as a means of investigating re-infestation rates of human dwellings. Geometric morphometrics have been widely applied in the study of Triatominae polymorphisms at species and population levels. This study characterizes morphometric differences between sylvatic and peridomestic populations, as well as between sexes in T. brasiliensis specimens from Jaguaruana, Ceará, in northeastern Brazil. No differences in either the shape or size of the cephalic capsule were apparent between sexes or ecotopes. However, the wings showed differentiation in shape and size. Sexual dimorphism was detected, with females presenting significantly higher values and conformations. Size differentiation was also evident, with sylvatic specimens being generally larger than peridomestic examples. These results indicate that differences in the wings of T. brasiliensis may be related to the existence of phenotypic plasticity, and variations in size and shape may be associated with different ecotopes, possibly as a result of conditions in each micro-habitat, such as temperature, relative humidity, food supply and density. © 2012 The Royal Entomological Society.

  11. Glycosylation of Vanillin and 8-Nordihydrocapsaicin by Cultured <em>Eucalyptus perrinianaem> Cells

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    Naoji Kubota

    2012-05-01

    Full Text Available Glycosylation of vanilloids such as vanillin and 8-nordihydrocapsaicin by cultured plant cells of <em>Eucalyptus perrinianaem> was studied. Vanillin was converted into vanillin 4-<em>O>-b-D-glucopyranoside, vanillyl alcohol, and 4-<em>O>-b-D-glucopyranosylvanillyl alcohol by <em>E. perriniana em>cells. Incubation of cultured <em>E. perrinianaem> cells with 8-nor- dihydrocapsaicin gave 8-nordihydrocapsaicin 4-<em>O>-b-D-glucopyranoside and 8-nordihydro- capsaicin 4-<em>O>-b-D-gentiobioside.

  12. Avaliação de óleos essenciais de plantas aromáticas com atividade inseticida em Triatoma infestans (Klug, 1834 (Hemiptera: Reduviidae Evaluation of essential oils with insecticidal activity in Triatoma infestans (Klug, 1834 (Hemiptera: Reduviidae

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    Silvio Favero

    2011-09-01

    Full Text Available A doença de Chagas é um dos maiores problemas de saúde pública na América Latina. Neste trabalho, foi avaliada a ação inseticida de óleos essenciais de Anacardium humile Cymbopogon nardus, Ocimum basilicum e Eucalyptus urograndis em ninfas do 3° e 4° estádios de desenvolvimento de Triatoma infestans por ação tópica e pressão a vapor (fumigação. Para o teste tópico foram realizadas duas repetições cada uma com cinco ninfas de 3° e 4° estádios de desenvolvimento de T. infestans. Foi aplicado 1µL de cada concentração na região dorsal de cada indivíduo. Para o teste de fumigação foram utilizados potes de 2 L com tampa vedante. Os óleos essenciais de O. gratissimum e E. urograndis apresentaram atividade inseticida para T. infestans, e A. humile e C. nardus apresentaram em média 5 e 15% de mortalidade, respectivamente. Para fumigação, somente o óleo de E. urograndis teve efeito, com 100% de mortalidade sobre T. infestans, sendo que para os outros óleos não foi observado efeito de fumigação. Esse trabalho relata pela primeira vez a ação inseticida de E. urograndis em potes fumígenos para o controle de triatomíneos. Os resultados são importantes devido à busca por novos modelos moleculares com ação inseticida em triatomíneos resistentes aos inseticidas sintéticos.Chagas disease is a major public health problem in Latin America. This work aimed to evaluate the insecticide action of essential oils of Anacardium humile, Cymbopogon nardus, Ocimum basilicum and Eucalyptus urograndis on Triatoma infestans nymphs of third and fourth stages of development by topic action and steam pressure (fumigation. Two repetitions were performed at the topic test with five nymphs of third and fourth stages of development of T. infestans. It was applied 1µL of each concentration on the dorsal region of each nymph. Two-liter pots with sealing covers were used at the fumigation test. The essential oils of O. gratissimum and E

  13. First record of Pulvinaria urbicola Cockerell (Hemiptera: Coccidae) from India, with a key to the Indian species of Pulvinaria Targioni Tozzetti.

    Science.gov (United States)

    Joshi, Sunil

    2017-02-23

    The notoriously destructive and invasive soft scale, Pulvinaria urbicola Cockerell (Hemiptera: Coccidae), is recorded for the first time from India. This scale, with variable morphological appearance and similarities with other known scales of the same genus established in India, is redescribed to facilitate its identification and separation from other similar species. Information on its host range, natural enemies and distribution is provided. Management options in the event of an outbreak are discussed briefly. A key to the species of Pulvinaria Targioni Tozzetti in India is also provided. This new arrival warrants special attention in India as it is a potentially damaging plant pest and has a broad host range across many plant families.

  14. New data on polymorphism of the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae) from the island of São Miguel (Azores).

    Science.gov (United States)

    Borges, Paulo A V; Rodrigues, Ana S B; Silva, Sara E; Seabra, Sofia G; Paulo, OctÁvio S; Quartau, JosÉ A

    2018-01-02

    The meadow spittlebug, Philaenus spumarius (L.) (Hemiptera, Aphrophoridae) is a widespread insect species in the Holarctic region, exhibiting a dorsal colour balanced polymorphism. In the Azores the species is known from high elevations in Terceira and São Miguel islands. A sample of 235 individuals from Pico da Vara and Graminhais protected areas (São Miguel, Azores) (between 645 and 935 m a.s.l.), collected in 2000 and 2017, showed a remarkable high frequency of the melanic morphs flavicollis (FLA) and quadrimaculatus (QUA). In addition, a high frequency of melanics was observed in males. We explore the hypotheses for the origin of Azorean colonization and for the high proportion of melanism in the Azorean populations.

  15. Life History of the Camelthorn Gall Leafhopper, Scenergates viridis (Vilbaste (Hemiptera, Cicadellidae

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    Roman Rakitov

    2012-01-01

    Full Text Available The world’s only member of Hemiptera Auchenorrhyncha known to form true galls, the leafhopper Scenergates viridis (Vilbaste (Cicadellidae, transforms leaves of camelthorn (Alhagi maurorum Medikus, Fabaceae into pod-like chambers, up to 35 mm long, inside which individual leafhoppers develop, mate, and lay eggs. At the study site 40 km SE of Bukhara (Uzbekistan, two generations develop annually. First-instar nymphs cause young leaves to fold along the midrib. The subsequent development takes place inside the tightly closed growing gall, plugged at both ends with a mixture of leafhopper excrement, brochosomes, and crushed exuviae. These plugs act as mechanical barriers and sticky traps for intruders. The inner surface of the gall, lined with brochosomes and wax platelets, is hydrophobic. Adult males emerge from their galls and squeeze into female galls. Fertilized females insert an average of 146 eggs under the gall’s inner epidermis and remain inside, possibly protecting the brood, until they die. The walls of the galls containing eggs are approximately three times thicker than regular leaves. The galls are subject to predation by Gelechiidae caterpillars; the eggs of the leafhopper are parasitized by two species of Trichogrammatidae and one Mymaridae (Hymenoptera, and its larvae by one species of Pipunculidae (Diptera.

  16. Preferencia alimenticia del ácaro depredador <em>Balaustium> sp. en condiciones controladas

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    Muñoz Karen

    2009-04-01

    Full Text Available

    Se evaluó la preferencia de presas de <em>Balaustium> sp., enemigo natural de diferentes artrópodos plaga, y el cual es nativo de la Sabana de Bogotá. En unidades experimentales construidas con foliolos de plantas de rosa se colocaron independientemente individuos de <em>Balaustium> sp. y se registró el número de presas consumidas. De esta manera se determinó la preferencia de los tres estados móviles del ácaro depredador <em>Balaustium> sp. por diferentes edades de tres presas. Las especies y edades de las presas estudiadas fueron: huevos, ninfas y adultos de <em>Trialeurodes vaporariorumem>, huevos, ninfas y adultos de <em>Tetranychus urticaeem>, y larvas de primer y segundo instar y adultos de <em>Frankliniella occidentalisem>. Los estados menos desarrollados fueron preferidos, aunque se observó que los adultos del depredador tienen gran habilidad para consumir adultos de <em>T. vaporariorumem>. La presa preferida por las larvas de <em>Balaustium> sp. fue los huevos de <em>T. urticaeem> con una proporción de consumo de 0,54 de los huevos que se ofrecieron de esta presa; las deutoninfas del depredador eligieron huevos de <em>T. vaporariorumem> (0,537 o de <em>T. urticaeem> (0,497 y los adultos de <em>Balaustium> sp. prefrieron los huevos de <em>T. vaporariorumem> (0,588.

  17. Ultrasound-Assisted Extraction of Carnosic Acid and Rosmarinic Acid Using Ionic Liquid Solution from <em>Rosmarinus officinalisem>>

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    Chunjian Zhao

    2012-09-01

    Full Text Available Ionic liquid based, ultrasound-assisted extraction was successfully applied to the extraction of phenolcarboxylic acids, carnosic acid and rosmarinic acid, from <em>Rosmarinus officinalisem>. Eight ionic liquids, with different cations and anions, were investigated in this work and [C8mim]Br was selected as the optimal solvent. Ultrasound extraction parameters, including soaking time, solid–liquid ratio, ultrasound power and time, and the number of extraction cycles, were discussed by single factor experiments and the main influence factors were optimized by response surface methodology. The proposed approach was demonstrated as having higher efficiency, shorter extraction time and as a new alternative for the extraction of carnosic acid and rosmarinic acid from <em>R. officinalisem>> em>compared with traditional reference extraction methods. Ionic liquids are considered to be green solvents, in the ultrasound-assisted extraction of key chemicals from medicinal plants, and show great potential.

  18. Descriptions of the immature stages and new host plant records of Notozulia entreriana (Berg) (Hemiptera: Cercopidae) pests of grasses in subtropical areas of the Americas.

    Science.gov (United States)

    Foieri, Alvaro; Lenicov, Ana M Marino De Remes; Virla, Eduardo G

    2016-04-11

    Notozulia entreriana (Berg) (Hemiptera: Cercopidae) is one of the most common spittlebugs inhabiting the subtropical region of the America, inflicting important economic damage to grass crops. The immature stages are described and illustrated; the main characteristics that distinguish instars are the body size, color, number of flagellomeres, and number of tibial and metatarsomere spines. A key for identification of nymphs is provided as a tool to develop field studies.  Nine host plants, all belonging to Poaceae, are recorded as breeding and feeding host plants from different localities in northern Argentina.

  19. Antioxidant Profile of <em>Trifolium pratenseem> L.

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    Heidy Schwartsova

    2012-09-01

    Full Text Available In order to examine the antioxidant properties of five different extracts of <em>Trifolium pratenseem> L. (Leguminosae leaves, various assays which measure free radical scavenging ability were carried out: 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, superoxide anion and nitric oxide radical scavenger capacity tests and lipid peroxidation assay. In all of the tests, only the H2O and (to some extent the EtOAc extracts showed a potent antioxidant effect compared with BHT and BHA, well-known synthetic antioxidants. In addition, <em>in vivo em>experiments were conducted with antioxidant systems (activities of GSHPx, GSHR, Px, CAT, XOD, GSH content and intensity of LPx in liver homogenate and blood of mice after their treatment with extracts of <em>T. pratenseem> leaves, or in combination with CCl4. Besides, in the extracts examined the total phenolic and flavonoid amounts were also determined, together with presence of the selected flavonoids: quercetin, luteolin, apigenin, naringenin and kaempferol, which were studied using a HPLC-DAD technique. HPLC-DAD analysis showed a noticeable content of natural products according to which the examined <em>Trifolium pratenseem> species could well be regarded as a promising new source of bioactive natural compounds, which can be used both as a food supplement and a remedy.

  20. The impact of some environmental factors on the fecundity of phenacoccus solenopsis tinsley (hemiptera: pseudococcidae): a serious pest of cotton and other crops

    International Nuclear Information System (INIS)

    Abbas, G.; Arif, M.J.; Aslam, M.

    2010-01-01

    Phenacoccus solenopsis Tinsley (Hemiptera: Sternorrhyncha: Pseudococcidae) was first recorded on cultivated cotton from Texas, USA in 1991. Since 2005, this New World species has emerged as serious pest of cotton in Pakistan and India, and is now a serious threat to cotton in China and other cotton-growing countries worldwide. The species is polyphagous and invasive, and can attack many other economic crops. So far, it has been reported from 173 species in 54 plant families, and from 26 countries in different ecological zones. The study found that host plant species and meteorological conditions had significant effects, whereas locality had no significant effect on the fecundity of the mealybug. (author)

  1. Bioassay-Guided Antidiabetic Study of <em>Phaleria macrocarpaem> Fruit Extract

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    Mohd Z. Asmawi

    2012-04-01

    Full Text Available An earlier anti-hyperglycemic study with serial crude extracts of <em>Phaleria macrocarpaem> (PM fruit indicated methanol extract (ME as the most effective. In the present investigation, the methanol extract was further fractionated to obtain chloroform (CF, ethyl acetate (EAF, <em>n>-butanol (NBF and aqueous (AF fractions, which were tested for antidiabetic activity. The NBF reduced blood glucose (<em>p> < 0.05 15 min after administration, in an intraperitoneal glucose tolerance test (IPGTT similar to metformin. Moreover, it lowered blood glucose in diabetic rats by 66.67% (<em>p> em>< 0.05, similar to metformin (51.11%, glibenclamide (66.67% and insulin (71.43% after a 12-day treatment, hence considered to be the most active fraction. Further fractionation of NBF yielded sub-fractions I (SFI and II (SFII, and only SFI lowered blood glucose (<em>p em>< 0.05, in IPGTT similar to glibenclamide. The ME, NBF, and SFI correspondingly lowered plasma insulin (<em>p em>< 0.05 and dose-dependently inhibited glucose transport across isolated rat jejunum implying an extra-pancreatic mechanism. Phytochemical screening showed the presence of flavonoids, terpenes and tannins, in ME, NBF and SFI, and LC-MS analyses revealed 9.52%, 33.30% and 22.50% mangiferin respectively. PM fruit possesses anti-hyperglycemic effect, exerted probably through extra-pancreatic action. Magniferin, contained therein may be responsible for this reported activity.

  2. A new whitefly genus and species, Himalayaleyrodes sarcococcae Dubey (Hemiptera: Aleyrodidae) infesting Christmas box (Buxaceae) in Western Himalaya, India.

    Science.gov (United States)

    Dubey, Anil Kumar; Singh, Sudhir

    2017-05-24

    Puparia, immatures and adults of a new whitefly genus and species, Himalayaleyrodes sarcococcae Dubey are described from the Western Himalaya, India. The new species was found feeding on leaves as well as on green stems of Sarcococca saligna (D. Don) Muell. Biological information, habitus, line arts, photomicrographs of holotype and Scanning Electron Microscope (SEM) images are given for this new taxon. Also, we discuss similarities and dissimilarities of puparia of the new genus with its closely related genus Bemisia Quaintance & Baker. Feeding behaviour is discussed.

  3. Primer registro de “la mosca negra de los cítricos” Aleurocanthus woglumi (Hemiptera: Aleyrodidae en la Argentina

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    Silvia N. LÓPEZ

    2011-01-01

    Full Text Available Aleurocanthus woglumi Ashby es una especie polífaga que ataca principalmente cultivos de Citrus spp. El objetivo de este trabajo es dar a conocer la primera detección de A. woglumi en Argentina. Los especímenes estudiados fueron recolectados en cultivos de pomelo en Tres Lagunas, departamento de Pilagá, Formosa, en febrero de 2011.

  4. Seasonal phenology, spatial distribution, and sampling plan for the invasive mealybug Phenacoccus peruvianus (Hemiptera: Pseudococcidae).

    Science.gov (United States)

    Beltrá, A; Garcia-Marí, F; Soto, A

    2013-06-01

    Phlenacoccus peruvianus Granara de Willink (Hemiptera: Pseudococcidae) is an invasive mealybug of Neotropical origin. In recent years it has invaded the Mediterranean Basin causing significant damages in bougainvillea and other ornamental plants. This article examines its phenology, location on the plant and spatial distribution, and presents a sampling plan to determine P. peruvianus population density for the management of this mealybug in southern Europe. Six urban green spaces with bougainvillea plants were periodically surveyed between March 2008 and September 2010 in eastern Spain, sampling bracts, leaves, and twigs. Our results show that P. peruvianus abundance was high in spring and summer, declining to almost undetectable levels in autumn and winter. The mealybugs showed a preference for settling on bracts and there were no significant migrations between plant organs. P. peruvianus showed a highly aggregated distribution on bracts, leaves, and twigs. We recommend abinomial sampling of 200 leaves and an action threshold of 55% infested leaves for integrated pest management purposes on urban landscapes and enumerative sampling for ornamental nursery management and additional biological studies.

  5. Alimentazione di <em>Marmota marmotaem> in praterie altimontane delle dolomiti bellunesi

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    Alessandro Rudatis

    2006-03-01

    Full Text Available Abstract The diet of <em>Marmota marmotaem> in the mountain prairie of south-eastern Italian Alps. Diet composition of two family groups of alpine marmots was investigated in two areas of the Agordino’s Dolomites (Italian Alps in June-September 2001, by means of microscopic analysis of faeces and of direct observation of feeding activity. During the whole period of activity, a high consume of Angiosperms was confirmed, especially plants in flower; among them the “graminoids” seemed to play an important role only during the initial part of the active period. Generally vegetative parts predominated over flowers. The ingestion of animal preys was not confirmed by the analysis of droppings. Comparing diet composition of the two groups, Graminaceae (<em>Poa>, <em>Phleum>, Compositae (<em>Achillea>, Cyperaceae/Juncaceae, Leguminosae (<em>Anthyllis>, Rosaceae, and Labiatae (<em>Prunella>, <em>Stachys> formed the bulk of marmot diet in the study areas. Diet showed low diversity considering the abundance of plant species in the surrounding environment. Food resources were probably used in relation to their easy digestibility, with a high content in proteins, sugar and water. The knowledge of vegetation features in relation to marmot trophic habits can represent a useful tool for the management of this species. Riassunto Il regime alimentare di due gruppi di Marmotta alpina è stato studiato in giugno-settembre 2001 in due aree delle Dolomiti agordine (SE Italia, attraverso l’analisi microscopica delle feci e l’osservazione diretta dell’attività alimentare. Durante tutto il periodo di attività si è notato un forte consumo di Angiosperme, specialmente piante a fiore, mentre le ”graminoidi” sembra giochino un ruolo importante all’inizio della stagione. In generale le parti vegetali predominano sui fiori. L’ingestione di prede animali non è stata

  6. <em>Ipomoea aquaticaem> Extract Shows Protective Action Against Thioacetamide-Induced Hepatotoxicity

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    A. Hamid A. Hadi

    2012-05-01

    Full Text Available In the Indian system of traditional medicine (Ayurveda it is recommended to consume <em>Ipomoea em>aquatica> to mitigate disorders like jaundice. In this study, the protective effects of ethanol extract of <em>I. aquaticaem> against liver damage were evaluated in thioacetamide (TAA-induced chronic hepatotoxicity in rats. There was no sign of toxicity in the acute toxicity study, in which Sprague-Dawley (SD rats were orally fed with <em>I. aquaticaem> (250 and 500 mg/kg for two months along with administration of TAA (i.p injection 200 mg/kg three times a week for two months. The results showed that the treatment of <em>I. aquaticaem> significantly lowered the TAA-induced serum levels of hepatic enzyme markers (ALP, ALT, AST, protein, albumin, bilirubin and prothrombin time. The hepatic content of activities and expressions SOD and CAT that were reduced by TAA were brought back to control levels by the plant extract supplement. Meanwhile, the rise in MDA level in the TAA receiving groups also were significantly reduced by <em>I. aquaticaem> treatment. Histopathology of hepatic tissues by H&E and Masson trichrome stains displayed that <em>I. aquaticaem> has reduced the incidence of liver lesions, including hepatic cells cloudy swelling, infiltration, hepatic necrosis, and fibrous connective tissue proliferation induced by TAA in rats. Therefore, the results of this study show that the protective effect of <em>I. aquaticaem> in TAA-induced liver damage might be contributed to its modulation on detoxification enzymes and its antioxidant and free radical scavenger effects. Moreover, it confirms a scientific basis for the traditional use of <em>I. aquaticaem> for the treatment of liver disorders.

  7. Classe social: conceitos e esquemas operacionais em pesquisa em saude

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    Rita Barradas Barata

    2013-08-01

    Full Text Available Discute-se a utilização do conceito de classe em pesquisas em saúde, as diferentes abordagens sociológicas de estratificação social e de estrutura de classes, o potencial explicativo do conceito em estudos de determinação social e desigualdades em saúde, os modelos de operacionalização elaborados para uso em pesquisas sociológicas, demográficas ou de saúde e os limites e possibilidades desses modelos. Foram destacados quatro modelos de operacionalização: de Singer para estudo da distribuição de renda no Brasil, adaptado por Barros para uso em pesquisas epidemiológicas; de Bronfman & Tuirán para o censo demográfico mexicano, adaptado por Lombardi et al para pesquisas epidemiológicas; de Goldthorpe para estudos socioeconômicos ingleses, adaptado pela Sociedade Espanhola de Epidemiologia; e o modelo de Wright para pesquisa em sociologia e ciência política, também usado em inquéritos populacionais em saúde. Em conclusão, conceitualmente cada um dos modelos apresentados é coerente com a concepção teórica que os embasam, mas não há como optar por qualquer deles, descartando os demais.

  8. Identification of a sex pheromone of the chrysanthemum lace bug Corythucha marmorata (Hemiptera: Tingidae).

    Science.gov (United States)

    Watanabe, Kisaki; Shimizu, Nobuhiro

    2017-08-04

    Although the nymphs of Corythucha marmorata form clusters on the undersides of host plant leaves, as frequently observed for Hemiptera, the adults are scattered in the vicinity of the nymph population. By investigating the biological activities of volatile secretions from the adult, we found that the secretions activated male mounting behaviour. A chemical analysis revealed that borneol was a common component of the secretions from both sexes. The absolute configuration of the natural product was the (+)-enantiomer of borneol and the optical isomer was undetectable. Although (+)-borneol showed significant sex pheromone activity against males, the antipode (-)-borneol also induced sex pheromone activity, albeit only slightly. Males may not have a strict identification mechanism based on stereochemistry. To verify the origin of this sex pheromone, we analysed the components of the essential oil of the leaves of Solidago canadensis L. (Compositae: Asteraceae), a host plant; bornyl acetate was detected to be a major component. The plant-produced bornyl acetate had different stereochemistry from the sex pheromone. The results suggested that the adults do not utilise the secondary metabolites of plants but biosynthesise this sex pheromone themselves. This is the first report on sex pheromone identification in Tingidae.

  9. Alberprosenia malheiroi Serra, Atzingen & Serra, 1987 (Hemiptera, Reduviidae: redescrição e bionomia

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    Rodolfo U. Carcavallo

    1995-12-01

    Full Text Available Sobre Alberprosenia malheiroi n. sp. deu-se a conhecer uma diagnose resumida em 1980, porém sem valor bibliográfico. Em 1987 publicou-se a mesma diagnose anexando uma foto e alguns comentários, porém sem realizar uma descrição formal. Descreve-se para esta espécie, os adultos, os estádios imaturos, determina-se a série sintípica e apresenta-se dados bionômicos e de criação em insetário. As diferenças mais evidentes com A. goyovargasi, a única espécie que se conhecia do gênero até então, são a coloração geral negra, o espaço interocular maior que o tamanho de um olho visto dorsalmente, os tubérculos do colar com o ápice agudo e o tamanho maior, na nova espécie, quase o dobro do da primeira espécie. Os ovos são pequenos, fixados ao substrato em grupos de 3 ou 4, elipsóides, não achatados lateralmente, com o opérculo proeminente, convexo, sem estruturas evidentes. As ninfas apresentam em todos os estádios caracteres típicos do gênero e da tribo, com região anteocular menos longa que a post-ocular e característica pilosidade do tegumento que vai se acentuando a cada estádio. A. malheiroi n. sp. foi capturado em ecótopos silvestres em palmeiras em floresta no Estado do Pará, associados com morcegos ou aves. Nenhum dos exemplares estava infectado com Trypanosoma cruzi. Esses triatomíneos foram mantidos em insetários a ± 25°C e ± 60% UR, são insetos ágeis e voam com relativa facilidade. Alimentaram-se bem em pombos e morcegos e não aceitaram alimentação em ratos, camundongos ou hamsters. O período de incubação dos ovos foi em média treze dias e o tempo de evolução do período ninfal foi em média cento e trinta e dois dias.

  10. KEBERADAAN DYSMICOCCUS BREVIPES (COCKERELL (HEMIPTERA: PSEUDOCOCCIDAE SEBAGAI VEKTOR PINEAPPLE MEALYBUG WILT-ASSOCIATED VIRUS (PMWAV PADA TANAMAN NANAS

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    Dewi Sartiami

    2006-04-01

    Full Text Available Mealybug can almost be found in all pineapple fields (Ananas comosus (Linnaeus. The insect is known to be a vector of Pineapple Mealybug wilt-associated Virus (PMWaV. The insect samples taken from pineapple in Bunihayu, Jalancagak, Subang, West Java, were identified in laboratory. Mealybug-ant symbionts were also taken. The ability of this ant to carry the mealybugs from colony reared on kabocha (Cucurbita maxima to pineapple was also tested at green house level. Only one spesies of mealybug was found on pineapple, i.e. Dysmicoccus brevipes (Cockerell (Hemiptera: Pseudococcidae. The mealybugs were found to colonize root, basal of stem and the leaf. Eight ant species were found to be associated with mealybug. There are four species belongs to Pseudolasius genera, two species Cardiocondyla genera, Paratrechina sp. and Dorylus sp. Paratrechina sp. showed the ability to carry D. brevipes from kabocha population to pineapple. Therefore the ants should also be controlled in the total management of PMWav.

  11. Identification guide to Nordic aphids associated with mosses, horsetails and ferns (Bryophyta, Equisetophyta, Polypodiophyta (Insecta, Hemiptera, Aphidoidea

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    Anders Christian Albrecht

    2015-10-01

    Full Text Available Keys and diagnoses of North European aphids (Hemiptera, Aphidoidea associated with mosses, horsetails and ferns are given, based on fresh and freeze-dried material. Numerous externally visible and thus informative characters, that are absent in cleared, slide-mounted specimens, such as body shape colours, wax coating and pattern etc., are utilized. Most of the species are illustrated by photographs of live specimens and drawings. Root-feeding species living in the moss layer or otherwise often present in moss samples are also included, even if their hosts were spermatophytes. The combination of colour images and diagnoses, utilizing easily observed characters, allows the identification of a large number of species already in the field, and many more at home with the aid of a stereo microscope. Host plant relationships and association with ants are summarised, including new records. Brief accounts on aphid life cycles, freeze-drying preparation techniques, etc. are also given to support the use of the keys.

  12. Factors affecting water strider (Hemiptera: Gerridae) mercury concentrations in lotic systems

    Energy Technology Data Exchange (ETDEWEB)

    Jardine, T.D.; Kidd, K.A.; Cunjak, R.A.; Arp, P.A. [University of New Brunswick, St John, NB (Canada). Canadian Rivers Institute

    2009-07-15

    Water striders (Hemiptera: Gerridae) have been considered as a potential sentinel for mercury (Hg) contamination of freshwater ecosystems, yet little is known about factors that control Hg concentrations in this invertebrate. Striders were collected from 80 streams and rivers in New Brunswick, Canada, in August and September of 2004 through 2007 to assess the influence of factors such as diet, water chemistry, and proximity to point sources on Hg concentrations in this organism. Higher than average Hg concentrations were observed in the southwest and Grand Lake regions of the province, the latter being the location of a coal-fired power plant that is a source of Hg (similar to 100 kg annually), with elevated Hg concentrations in the lichen Old Man's Beard (Usnea spp.) in its immediate vicinity. Across all streams, pH and total organic carbon of water were relatively weak predictors of strider Hg concentrations. Female striders that were larger in body size than males had significantly lower Hg concentrations within sites, suggestive of growth dilution. There was no relationship between percent aquatic carbon in the diet and Hg concentrations in striders. For those striders feeding solely on terrestrial carbon, Hg concentrations were higher in animals occupying a higher trophic level. Mercury concentrations were highly variable in striders collected monthly over two growing seasons, suggesting short-term changes in Hg availability. These measurements highlight the importance of considering both deposition and postdepositional processes in assessing Hg bioaccumulation in this species.

  13. Seletividade fisiológica de inseticidas aos inimigos naturais de Plutella xylostella (L. (Lepidoptera: Plutellidae em brássicas Insecticide physiological selectivity to natural enemies of Plutella xylostella (L. (Lepidoptera: Plutellidae in Brassicae

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    Leandro Bacci

    2009-01-01

    Full Text Available A conservação de inimigos naturais é um componente fundamental no manejo integrado de pragas. Neste trabalho, estudou-se a seletividade de sete inseticidas usados no manejo de Plutella xylostella (L. (Lepidoptera: Plutellidae ao parasitóide Oomyzus sokolowskii (Kurdjumov (Hymenoptera: Eulophidae e aos predadores Discodon sp. (Coleoptera: Cantharidae e Lasiochilus sp. (Hemiptera: Anthocoridae. Os organofosforados metamidofós e paratiom metílico e o carbamato carbaril foram altamente tóxicos aos três inimigos naturais estudados. Dimetoato apresentou seletividade em favor do Discodon sp., na dose e subdose. O inseticida acefato apresentou alto impacto ao Discodon sp., no momento da aplicação e seu efeito foi reduzido com a metade da concentração do princípio ativo. Deltametrina apresentou seletividade à O. sokolowskii nas duas dosagens. Já permetrina foi seletiva a Lasiochilus sp., somente na subdose. Discodon sp. foi mais tolerante à dose e subdose do dimetoato e à subdose do acefato do que O. sokolowskii e Lasiochilus sp. O predador Lasiochilus sp. foi mais tolerante à dose e subdose da permetrina do que O. sokolowskii e Discodon sp. O parasitóide O. sokolowskii foi mais tolerante à dose e subdose da deltametrina do que Lasiochilus sp. e Discodon sp. Os resultados desta pesquisa foram obtidos em condições de exposição extrema, portanto indicam que os inseticidas seletivos possivelmente não serão prejudiciais aos inimigos naturais, em condições de campo.The conservation of natural enemies is a fundamental component in the integrated pest management. In this work, one studied the selectivity of seven insecticides, used in the management of Plutella xylostella (L. (Lepidoptera: Plutellidae, to the parasitoid Oomyzus sokolowskii (Kurdjumov (Hymenoptera: Eulophidae, and to the predators Discodon sp. (Coleoptera: Cantharidae and Lasiochilus sp. (Hemiptera: Anthocoridae. The organophosphates methamidophos and methyl parathion

  14. The diet of the fox (<em>Vulpes vulpesem> in woodlands of Orobie Alps (Lombardy region, Northern Italy / Alimentazione della Volpe (<em>Vulpes vulpesem> in aree boscate delle Alpi Orobie

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    Marco Cantini

    1991-07-01

    Full Text Available Abstract The diet of the fox was investigated by analysis of 273 scats, collected along standard trails from April to November 1987 and 1988. Food habits of foxes were described for three altitudinal ranges. Mammals, mainly <em>Clethrionomys glareolusem> and <em>Microtus multiplexem>, were the staple food (percentage of frequency 42.8%, followed by fruits and other vegetables (26.7% and 37.3% respectively. Birds, Invertebrates (mainly Insects and garbage were little eaten. The game species (ungulates, hares, pheasants occurred with a low frequency (8.4% in the diet. The trophic niche breadth varied little through the altitudinal ranges and the seasons. The trophic niche overlap between the fox and the genus <em>Martes> (190 scats of <em>M. martesem> and <em>M. foinaem> were examined is relatively wide (O=0.868. Riassunto La dieta della Volpe (<em>Vulpes vulpesem> in aree boscate delle Alpi Orobie (Val Lesina è stata indagata nel periodo aprile-novembre 1987 e 1988 mediante l'analisi di 273 feci, raccolte lungo percorsi-campione ricadenti in tre piani vegetazionali. I Mammiferi, in particolare <em>Clethrionomys glareolusem> e <em>Microtus multiplexem>, sono la componente principale della dieta (frequenza percentuale 42,8%. Rilevante è anche il consumo di frutti (soprattutto in estate e autunno e di altri vegetali (26,7% e 37,3% rispettivamente, mentre poco frequente è quello di Uccelli, Invertebrati e rifiuti. Complessivamente ridotta è l'azione predatoria della Volpe nei confronti delle specie di interesse venatorio (Ungulati, lepri, Galliformi. L'ampiezza della nicchia trofica mostra modeste variazioni stagionali e altitudinali. I1 grado di sovrapposizione tra la nicchia trofica della Volpe e quella del genere <em>Martes>, quest'ultima ricavata dall'analisi di 190 feci di Martora (<em>M. martesem> e Faina (<em>M. foinaem>, è elevato (O=0,868. Tuttavia, poiché in condizioni di

  15. Osservazioni in cattività sul ciclo stagionale del peso corporeo e sull'efficienza digestiva di <em>Pipistrellus kuhliiem> e <em>Hypsugo saviiem> (Chiroptera: Vespertilionidae

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    Gianna Dondini

    2003-10-01

    Full Text Available Molte specie di pipistrelli delle fasce climatiche temperato-fredde sono soggette a marcate variazioni stagionali di temperatura e disponibilità di cibo. L'accumulo di grasso in autunno è quindi un adattamento per trascorrere, in uno stato di profondo torpore definibile ibernazione, i mesi invernali, aumentando così la probabilità di sopravvivenza durante tale periodo. Nell?ambito di una attività pluriennale relativa alla raccolta, studio e, quando possibile, riabilitazione di pipistrelli in ambienti urbani, due esemplari di <em>Pipistrellus kuhliiem> (2 femmine e due di <em>Hypsugo saviiem> (1 maschio e 1 femmina, in entrambi casi giovani che ancora non avevano acquisito una sufficiente capacità nel volo e quindi non liberabili, sono stati raccolti nella pianura di Firenze durante l?estate del 1998 e mantenuti in condizioni di temperatura ambientale oscillante tra i 17 e i 22°C, in un contenitore di 150x40x30 cm. Ogni sera sono stati pesati, prima della somministrazione di cibo e acqua, con una bilancia elettronica con precisione di 0.1 g (modello Tanita 1479. L'alimentazione è stata a base di vermi della farina (<em>Tenebrio molitorem>. L?efficienza digestiva è calcolata nel seguente modo, su materiale disidratato: (quantità ingerita ? quantità escrementi/quantità ingerita*100. Per il calcolo di tale indice gli esemplari delle due specie sono stati separati e mantenuti per 24 ore a partire dalla successiva sera dell?ultima somministrazione, favorendo così lo svuotamento dell?intestino. Successivamente, per due giorni è stato fornito del cibo <em>ad libitumem>, pesando i singoli esemplari una volta terminata la fase di alimentazione, per determinare la quantità ingerita. Al termine abbiamo mantenuto gli esemplari a digiuno per 24 ore successive all?ultima somministrazione per permettere lo svuotamento dell?intestino. Gli escrementi raccolti sono stati posti in forno elettrico a 90 °C per 24 ore e successivamente pesati

  16. Net Energy, CO2 Emission and Land-Based Cost-Benefit Analyses of <em>Jatropha> em>Biodiesel: A Case Study of the Panzhihua Region of Sichuan Province in China

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    Xiangzheng Deng

    2012-06-01

    Full Text Available Bioenergy is currently regarded as a renewable energy source with a high growth potential. Forest-based biodiesel, with the significant advantage of not competing with grain production on cultivated land, has been considered as a promising substitute for diesel fuel by many countries, including China. Consequently, extracting biodiesel from <em>Jatropha> curcasem> has become a growing industry. However, many key issues related to the development of this industry are still not fully resolved and the prospects for this industry are complicated. The aim of this paper is to evaluate the net energy, CO2 emission, and cost efficiency of <em>Jatropha> biodiesel as a substitute fuel in China to help resolve some of the key issues by studying data from this region of China that is well suited to growing <em>Jatropha>. Our results show that: (1 <em>Jatropha> biodiesel is preferable for global warming mitigation over diesel fuel in terms of the carbon sink during <em>Jatropha> tree growth. (2 The net energy yield of <em>Jatropha> biodiesel is much lower than that of fossil fuel, induced by the high energy consumption during <em>Jatropha> plantation establishment and the conversion from seed oil to diesel fuel step. Therefore, the energy efficiencies of the production of <em>Jatropha> and its conversion to biodiesel need to be improved. (3 Due to current low profit and high risk in the study area, farmers have little incentive to continue or increase <em>Jatropha> production. (4 It is necessary to provide more subsidies and preferential policies for <em>Jatropha> plantations if this industry is to grow. It is also necessary for local government to set realistic objectives and make rational plans to choose proper sites for <em>Jatropha> biodiesel development and the work reported here should assist that effort. Future research focused on breading high-yield varieties, development of efficient field

  17. Threatening “the Good Order”: West Meets East in Cecil B. DeMille’s <em>The Cheatem> and John Updike’s <em>Terrorist>

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    Bradley M. Freeman

    2011-12-01

    Full Text Available

    Despite almost a hundred years of separation, both Cecil B. DeMille’s film <em>The Cheatem> (1915 and John Updike’s novel <em>Terrorist> (2006 deploy a clear-cut territorial divide between Western and Eastern spaces in order to envision a unified American space. These narratives superimpose a “natural” division on these historically opposed spaces and thereby suggest that any contact between these spaces will have dangerous consequences. These consequences include the potential dissolution and eventual destruction of American productivity, surveillance, and territorial integrity. DeMille’s film and Updike’s novel represent America as a nation-state that must be protected from the East. In 1915, <em>The Cheatem> warned against an interracial America and the upsurge in immigration that characterized the turn of the century. Nearly a century later, <em>Terrorist> presupposes an interracial America but still constructs an East that threatens the security of America. While registering the particular concerns of two distinct historical moments, these narratives represent a larger attempt in American aesthetics to imagine an East that jeopardizes the utopian possibilities of an overly idealized American space.

  18. <em>In Vitro em>Phytotoxicity and Antioxidant Activity of Selected Flavonoids

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    Rita Patrizia Aquino

    2012-05-01

    Full Text Available The knowledge of flavonoids involved in plant-plant interactions and their mechanisms of action are poor and, moreover, the structural characteristics required for these biological activities are scarcely known. The objective of this work was to study the possible <em>in vitro em>phytotoxic effects of 27 flavonoids on the germination and early radical growth of <em>Raphanus sativus em>L.> and <em>Lepidium sativumem> L., with the aim to evaluate the possible structure/activity relationship. Moreover, the antioxidant activity of the same compounds was also evaluated. Generally, in response to various tested flavonoids, germination was only slightly affected, whereas significant differences were observed in the activity of the various tested flavonoids against radical elongation. DPPH test confirms the antioxidant activity of luteolin, quercetin, catechol, morin, and catechin. The biological activity recorded is discussed in relation to the structure of compounds and their capability to interact with cell structures and physiology. No correlation was found between phytotoxic and antioxidant activities.

  19. Cicadas (Hemiptera: Cicadidae) from Camiguin of Mindanao Province and Dinagat Island in the Philippines, with a new genus and three new species.

    Science.gov (United States)

    Lee, Young June; Marshall, David C; Mohagan, Alma; Hill, Kathy B R

    2016-03-30

    This paper provides the first faunal checklist for the family Cicadidae (Hemiptera) from Camiguin of Mindanao Province and Dinagat Island in the Philippines, comprising ten species belonging to nine genera. Cryptotympana shillana Lee & Mohagan sp. nov., Orientopsaltria inermis (Stål, 1870), Purana crassinotata Lee, 2015, and Huechys parvula Haupt, 1924 are recorded for the first time from Camiguin. Platypleura dinagatensis Lee sp. nov., Chremistica kyoungheeae Lee, 2010, Dundubia vaginata (Fabricius, 1787), Oncotympana pallidiventris Stål, 1870, and Philipsalta nigrina Lee, Marshall & Hill sp. nov. are newly recorded from Dinagat Island. A new genus Philipsalta Lee, Marshall & Hill gen. nov. is erected. Huechysini Distant, 1905 syn. nov. is synonymized with Cicadettini Buckton, 1889. Information on geographic distributions of the Camiguin and Dinagat species is also provided.

  20. The Complete Mitochondrial Genome of Mindarus keteleerifoliae (Insecta: Hemiptera: Aphididae) and Comparison with Other Aphididae Insects.

    Science.gov (United States)

    Wang, Yuan; Chen, Jing; Jiang, Li-Yun; Qiao, Ge-Xia

    2015-12-17

    The mitogenome of Mindarus keteleerifoliae Zhang (Hemiptera: Aphididae) is a 15,199 bp circular molecule. The gene order and orientation of M. keteleerifoliae is similarly arranged to that of the ancestral insect of other aphid mitogenomes, and, a tRNA isomerism event maybe identified in the mitogenome of M. keteleerifoliae. The tRNA-Trp gene is coded in the J-strand and the same sequence in the N-strand codes for the tRNA-Ser gene. A similar phenomenon was also found in the mitogenome of Eriosoma lanigerum. However, whether tRNA isomers in aphids exist requires further study. Phylogenetic analyses, using all available protein-coding genes, support Mindarinae as the basal position of Aphididae. Two tribes of Aphidinae were recovered with high statistical significance. Characteristics of the M. keteleerifoliae mitogenome revealed distinct mitogenome structures and provided abundant phylogenetic signals, thus advancing our understanding of insect mitogenomic architecture and evolution. But, because only eight complete aphid mitogenomes, including M. keteleerifoliae, were published, future studies with larger taxon sampling sizes are necessary.

  1. Plant Essential Oils Synergize and Antagonize Toxicity of Different Conventional Insecticides against Myzus persicae (Hemiptera: Aphididae)

    Science.gov (United States)

    Faraone, Nicoletta; Hillier, N. Kirk; Cutler, G. Christopher

    2015-01-01

    Plant-derived products can play an important role in pest management programs. Essential oils from Lavandula angustifolia (lavender) and Thymus vulgaris (thyme) and their main constituents, linalool and thymol, respectively, were evaluated for insecticidal activity and synergistic action in combination with insecticides against green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). The essential oils and their main constituents exerted similar insecticidal activity when aphids were exposed by direct sprays, but were non-toxic by exposure to treated leaf discs. In synergism experiments, the toxicity of imidacloprid was synergized 16- to 20-fold by L. angustifolia and T. vulgaris essential oils, but far less synergism occurred with linalool and thymol, indicating that secondary constituents of the oils were probably responsible for the observed synergism. In contrast to results with imidacloprid, the insecticidal activity of spirotetramat was antagonized by L. angustifolia and T. vulgaris essential oils, and linalool and thymol. Our results demonstrate the potential of plant essential oils as synergists of insecticides, but show that antagonistic action against certain insecticides may occur. PMID:26010088

  2. Transferrin Family Genes in the Brown Planthopper, Nilaparvata lugens (Hemiptera: Delphacidae) in Response to Three Insecticides.

    Science.gov (United States)

    Wu, Shun-Fan; Li, Jian; Zhang, Yong; Gao, Cong-Fen

    2018-02-09

    Transferrins are involved in iron metabolism, immunity, xenobiotics tolerance, and development in eukaryotic organisms including insects. However, little is known about the relationship between transferrins and insecticide toxicology and resistance. Three transferrin family genes, NlTsf1, NlTsf2, and NlTsf3, of the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)a major insect pest of rice field in Asia, had been identified and characterized in this study. Quantitative polymerase chain reaction results demonstrated that NlTsf1 was significantly higher than the other two genes in different tissues. All of them were expressed at higher levels in abdomen and head than in antenna, leg, stylet, and thorax. Compared with the control, the expression of three N. lugens transferrin family genes decreased dramatically 24 h after treatment with buprofezin, pymetrozine and imidacloprid. Published by Oxford University Press on behalf of Entomological Society of America 2017. This work is written by (a) US Government employee(s) and is in the public domain in the US.

  3. Two-Spotted Ladybeetle Adalia bipunctata L. (Coleoptera: Coccinellidae): A Commercially Available Predator to Control Asian Citrus Psyllid Diaphorina citri (Hemiptera: Liviidae).

    Science.gov (United States)

    Khan, Azhar A; Qureshi, Jawwad A; Afzal, Muhammad; Stansly, Philip A

    2016-01-01

    The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae) is an economically important pest of citrus because it serves as a vector of the causal pathogens of huanglongbing (HLB) also known as citrus greening disease. The increased use of insecticides for control of D. citri negatively impacts several natural enemies including some effective ladybeetle species which are not available commercially. The two-spotted ladybeetle, Adalia bipunctata (Coleoptera: Coccinellidae) is found in some crop and forest ecosystems of Asia, Europe and North America and available commercially. It is known to attack aphids and mealybugs but there are no published records of feeding on psyllids. We evaluated suitability and preference of A. bipunctata for nymphs of D. citri compared to corn leaf aphid Rhopalosiphum maidis (Hemiptera: Aphididae) a global pest of cereal crops and prey for many predaceous insects. We also compared development and reproduction of A. bipunctata on these two species with frozen eggs of the Mediterranean flour moth Ephestia kuehniella (Lepidoptera: Pyralidae) at 25°C. Initially, more D. citri than R. maidis nymphs were consumed in the no-choice tests although final consumption by larva and adult of A. bipunctata did not differ in the choice and no-choice tests. Larval development was prolonged by one day on D. citri compared to R. maidis nymphs but did not differ between either of these diets and E. kuehniella. Larval survival to adult averaged 93-100% and was not impacted by diet. Adult life span did not differ between diets although those on D. citri and R. maidis nymphs weighed less and produced fewer but more fertile eggs than on E. kuehniella eggs. Significant reduction of D. citri nymphs averaging 54% was observed in colonies caged with adult A. bipunctata on field planted citrus. R° (net reproductive rate) was least for beetles fed R. maidis, but otherwise there were no significant differences in demographic parameters. Successful

  4. Distribuição espacial de populações de triatomíneos (Hemiptera: Reduviidae em palmeiras da espécie Mauritia flexuosa no Distrito Federal, Brasil Spatial distribution of Triatominae populations (Hemiptera: Reduviidae in Mauritia flexuosa palm trees in Federal District of Brazil

    Directory of Open Access Journals (Sweden)

    Rodrigo Gurgel-Gonçalves

    2004-06-01

    Full Text Available Para determinar e analisar a distribuição espacial de populações silvestres de triatomíneos no Distrito Federal, Brasil, foram amostradas 150 palmeiras da espécie Mauritia flexuosa em seis veredas de diferentes paisagens (silvestre, rural e periurbana na estação chuvosa. Os triatomíneos foram identificados morfologicamente, separados por sexo e estadio ninfal e examinados para verificar infecção por Trypanosoma cruzi e Trypanosoma rangeli. Vinte e oito (18,6% palmeiras estavam infestadas por Rhodnius neglectus e 14 (9,5% por Psammolestes tertius. A freqüência dessas espécies em palmeiras com e sem ninhos foi significativamente diferente, sendo superior nas palmeiras com ninhos de aves e mamíferos na copa. O maior número médio de insetos/palmeira foi observado nas áreas rurais, com estimativas de até 838 insetos/hectare. A composição etária das espécies apresentou um padrão diferente, Rhodnius neglectus com predomínio de ninfas e Psammolestes tertius com predomínio de adultos, sendo que muitos ovos de Rhodnius neglectus foram coletados indicando um evento reprodutivo em fevereiro de 2003. Entre os ninhos encontrados nas palmeiras, o do pássaro Phacellodomus ruber (Furnariidae apresentou as maiores abundâncias de triatomíneos, ocorrendo em 42% das palmeiras. A abundância relativa de Rhodnius neglectus e Psammolestes tertius foi maior em áreas rurais que possuíram maior número de ninhos nas palmeiras e menor densidade de palmeiras por hectare. Nenhum dos 96 triatomíneos examinados estava infectado por Trypanosoma cruzi ou Trypanosoma rangeli, apesar de encontrarmos infecção de Rhodnius neglectus por esses parasitas em estudos anteriores.To determine and analyze the distribution of Triatominae sylvatic populations in the Federal District of Brazil, 150 Mauritia flexuosa palm trees were sampled in six veredas of different landscapes (sylvatic, rural and peri-urban in the rainy season. Triatomines were

  5. Busca de estruturas em grandes escalas em altos redshifts

    Science.gov (United States)

    Boris, N. V.; Sodré, L., Jr.; Cypriano, E.

    2003-08-01

    A busca por estruturas em grandes escalas (aglomerados de galáxias, por exemplo) é um ativo tópico de pesquisas hoje em dia, pois a detecção de um único aglomerado em altos redshifts pode por vínculos fortes sobre os modelos cosmológicos. Neste projeto estamos fazendo uma busca de estruturas distantes em campos contendo pares de quasares próximos entre si em z Â3 0.9. Os pares de quasares foram extraídos do catálogo de Véron-Cetty & Véron (2001) e estão sendo observados com os telescópios: 2,2m da University of Hawaii (UH), 2,5m do Observatório de Las Campanas e com o GEMINI. Apresentamos aqui a análise preliminar de um par de quasares observado nos filtros i'(7800 Å) e z'(9500 Å) com o GEMINI. A cor (i'-z') mostrou-se útil para detectar objetos "early-type" em redshifts menores que 1.1. No estudo do par 131046+0006/J131055+0008, com redshift ~ 0.9, o uso deste método possibilitou a detecção de sete objetos candidatos a galáxias "early-type". Num mapa da distribuição projetada dos objetos para 22 escala. Um outro argumento em favor dessa hipótese é que eles obedecem uma relação do tipo Kormendy (raio equivalente X brilho superficial dentro desse raio), como a apresentada pelas galáxias elípticas em z = 0.

  6. Encapsulation-Induced Stress Helps <em>Saccharomyces cerevisiae em>Resist Convertible Lignocellulose Derived Inhibitors

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    Johan O. Westman

    2012-09-01

    Full Text Available The ability of macroencapsulated <em>Saccharomyces cerevisiae em>CBS8066<em> em>to withstand readily and not readily <em>in situem> convertible lignocellulose-derived inhibitors was investigated in anaerobic batch cultivations. It was shown that encapsulation increased the tolerance against readily convertible furan aldehyde inhibitors and to dilute acid spruce hydrolysate, but not to organic acid inhibitors that cannot be metabolized anaerobically. Gene expression analysis showed that the protective effect arising from the encapsulation is evident also on the transcriptome level, as the expression of the stress-related genes <em>YAP1em>, <em>ATR1em> and <em>FLR1em> was induced upon encapsulation. The transcript levels were increased due to encapsulation already in the medium without added inhibitors, indicating that the cells sensed low stress level arising from the encapsulation itself. We present a model, where the stress response is induced by nutrient limitation, that this helps the cells to cope with the increased stress added by a toxic medium, and that superficial cells in the capsules degrade convertible inhibitors, alleviating the inhibition for the cells deeper in the capsule.

  7. Morphological identification of the Soprano Pipistrelle (<em>Pipistrellus pygmaeusem> Leach, 1825 in Croatia.

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    Igor Pavlinić

    2008-07-01

    Full Text Available Abstract After the discovery of two different phonic types within the common pipistrelle (<em>Pipistrellus pipistrellusem>, mtDNA analysis confirmed the existence of two separate species named as common pipistrelle (<em>P. pipistrellusem> and soprano pipistrelle (<em>P. pygmaeusem>. The discrimination of these two cryptic species using external characters and measures has proved to be somewhat problematic. We examined two colonies of soprano pipistrelle from Donji Miholjac, Croatia. As a result, only two characters proved to be of help for field identification: wing venation (89% of cases and penis morphology and colour for males. The difference in length between the 2nd and 3rd phalanxes of the 3rd finger should be discarded as diagnostic trait between <em>P. pipistrellusem> and <em>P. pygmaeusem> in Croatia. Riassunto Identificazione su basi morfologiche del pipistrello pigmeo (<em>Pipistrellus pygmeausem>, Leach, 1825 in Croazia. A seguito della descrizione di due differenti "tipi fonici" nel pipistrello nano (<em>Pipistrellus pipistrellusem> e della successiva conferma su basi genetiche dell'esistenza di due specie distinte, designate come pipistrello nano (<em>P. pipistrellusem> e pipistrello pigmeo (<em>P. pygmaeusem>, la distinzione delle due specie in base a caratteristiche morfologiche esterne si è dimostrata un problema di difficile soluzione. Sulla base delle caratteristiche distintive e delle differenze biometriche proposte da altri Autori, sono state esaminate due colonie di pipistrello pigmeo a Donji Miholjac, in Croazia. I risultati ottenuti evidenziano che, tra tutti i potenziali caratteri sinora proposti, solo due risultano utili per un'identificazione diretta sul campo: la venatura delle ali, risultata utile alla discriminazione nell'89% degli esemplari analizzati, e la colorazione e morfologia del pene nei maschi. La

  8. Altitudinal distribution of the common longeared bat <em>Plecotus auritusem> (Linnaeus, 1758 and grey long-eared bat <em>Plecotus austriacusem> (J. B. Fischer, 1829 (Chiroptera, Vespertilionidae in the Tatra mountains (southern Poland

    Directory of Open Access Journals (Sweden)

    Krzysztof Piksa

    2006-03-01

    Full Text Available Riassunto Distribuzione altitudinale di Orecchione bruno (<em>Plecotus auritusem> e Orecchione meridionale (<em>Plecotus austriacusem> nei Monti Tatra (Polonia meridionale. Vengono riportati nuovi dati relativi alla distribuzione altitudinale nei Monti Tatra (Polonia meridionale di <em>Plecotus auritusem> e <em>P. austriacusem>. Tali segnalazioni incrementano le conoscenze relative alla presenza di questi chirotteri a quote elevate, in particolare per la Polonia. In inverno <em>P. auritusem> è stato rinvenuto a 1921 m s.l.m. mentre in estate è stato rinvenuto a 2250 m s.l.m.; in aggiunta, sono stati ritrovati resti ossei a 1929 m s.l.m. <em>P. austriacusem> è stato segnalato in ibernazione a 1294 m s.l.m.

  9. Molecular and morphological identification of  pistachio armored scale insects (Hemiptera: Diaspididae), with description of a new species.

    Science.gov (United States)

    Hosseininaveh, Fatemeh; Nozari, Jamasb; Kaydan, Mehmet Bora; Hosseininaveh, Vahid

    2016-12-01

    Members of the family Diaspididae (Hemiptera: Coccomorpha) can be devastating pests that suck parenchyma cell contents from crops and cause severe damage to pistachio trees (Pistacia vera L.). The current research collected and characterized diaspidid species from pistachio orchards in Kerman province, Iran, according to their morphological and molecular features. Lepidosaphes pistaciae Archangelskaya, Suturaspis davatchi (Balachowsky & Kaussari) and Melanaspis inopinata (Leonardi) are redescribed and a new species, Melanaspis pistaciae Hosseininaveh & Kaydan sp. n., is described. Phylogenetic trees based on molecular analysis of COI and 28S rDNA fragments placed all the species in separated clades and confirmed M. pistaciae as a new taxon which is concluded by morphological differences. Molecular analysis suggests non-monophyly of the populations of each species. Melanaspis pistaciae sp. n. has spread to most cultivated pistachio areas in Iran and has probably been misidentified as M. inopinata in the past. Further investigation of the biology of this species may lead to development of more effective approaches for controlling this pest.

  10. Action of Chitosan Against <em>Xanthomonas> Pathogenic Bacteria Isolated from <em>Euphorbia pulcherrimaem>

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    Yanli Wang

    2012-06-01

    Full Text Available The antibacterial activity and mechanism of two kinds of chitosan were investigated against twelve <em>Xanthomonas> strains recovered from <em>Euphorbia pulcherrimaem>. Results indicated that both chitosans markedly inhibited bacterial growth based on OD loss. Furthermore, the release of DNA and RNA from three selected strains was increased by both chitosans. However, the release of intracellular proteins was inhibited by both chitosans at different concentration and incubation times, except chitosan A at 0.1 mg/mL for 0.5 h incubation and 0.2 mg/mL for 2.0 h incubation increased the release of proteins, indicating the complexity of the interaction and cell membranes, which was affected by incubation time, bacterial species, chitosan type and concentration. Transmission electron microscopic observations revealed that chitosan caused changes in protoplast concentration and surface morphology. In some cells, the membranes and walls were badly distorted and disrupted, while other cells were enveloped by a thick and compact ribbon-like layer. The contrary influence on cell morphology may explain the differential effect in the release of material. In addition, scanning electron microscope and biofilm formation test revealed that both chitosans removed biofilm biomass. Overall, this study showed that membrane and biofilm play an important role in the antibacterial mechanism of chitosan.

  11. Study of the <em>in Vitroem> Antiplasmodial, Antileishmanial and Antitrypanosomal Activities of Medicinal Plants from Saudi Arabia

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    Nawal M. Al-Musayeib

    2012-09-01

    Full Text Available The present study investigated the <em>in vitroem> antiprotozoal activity of sixteen selected medicinal plants. Plant materials were extracted with methanol and screened <em>in vitroem> against erythrocytic schizonts of <em>Plasmodium falciparumem>, intracellular amastigotes of <em>Leishmania infantum em>and <em>Trypanosoma cruzi em>and free trypomastigotes of<em> T. bruceiem>. Cytotoxic activity was determined against MRC-5 cells to assess selectivity<em>. em>The criterion for activity was an IC50 < 10 µg/mL (4. Antiplasmodial activity was found in the<em> em>extracts of<em> em>>Prosopis julifloraem> and <em>Punica granatumem>. Antileishmanial activity<em> em>against <em>L. infantumem> was demonstrated in <em>Caralluma sinaicaem> and <em>Periploca aphylla.em> Amastigotes of<em> T. cruzi em>were affected by the methanol extract of<em> em>>Albizia lebbeckem>> em>pericarp, <em>Caralluma sinaicaem>,> Periploca aphylla em>and <em>Prosopius julifloraem>. Activity against<em> T. brucei em>was obtained in<em> em>>Prosopis julifloraem>. Cytotoxicity (MRC-5 IC50 < 10 µg/mL and hence non-specific activities were observed for<em> em>>Conocarpus lancifoliusem>.>

  12. Effects of temperature and food on the development of Dysdercus maurus Distant (Hemiptera, Pyrrhocoridae); Efeitos da temperatura e do alimento no desenvolvimento de Dysdercus maurus Distant (Hemiptera, Pyrrhocoridae)

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Fabio Souto [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil). Dept. de Ciencias Ambientais], e-mail: fbio_almeida@yahoo.com.br; Goncalves, Lenicio [Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropedica, RJ (Brazil). Dept. de Biologia Animal. Area de Biologia], e-mail: lencygon@globo.com

    2007-10-15

    Dysdercus maurus Distant, 1901 (Hemiptera, Pyrrhocoridae) is an important pest on Gossypium spp. (cotton tree), Citrus sinensis Osbeck (Rutaceae) and Citrus reticulata Blanco (Rutaceae) crops. This insect also feeds on seeds of Chorisia speciosa St. Hil. (Bombacaceae). This work aimed to evaluate the effects of temperature and food on the development of D. maurus. Eight treatments were carried out, in six of them bugs were fed with seeds of C. speciosa and kept at 15, 18, 20, 25 and 30 {+-} 1 deg C, 80 {+-} 3% RH and 12h photo phase or in laboratory conditions (23.5 {+-} 2.6 deg C, 73.3 {+-} 9.9 % RH), and in the other two treatments bugs were fed with seeds of cotton variety IAC-22 and kept at 25 or 30 deg C. In all treatments five immature stages were observed. The increase of temperature caused reduction in the developmental time. The temperature of 15 deg C disabled nymphal eclosion and was also lethal to those nymphs ecloded at other temperatures. The lower mortality of nymphs occurred in the temperature of 25 deg C with cotton as food (24.07%). The lower threshold temperature (Tb) occurred for the first instar (11.54 deg C) and the higher for the second instar (15.33 deg C). The females of D. maurus required more degree-days (329.93 degree-days) than males (300.49 degree-days) until adult emergence. (author)

  13. Infestation of Palm Trees by Triatomines (Hemiptera: Reduviidae in the State of Bahia, Brazil

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    Rodrigo Gurgel-Gonçalves

    2012-12-01

    Resumo. As palmeiras desempenham papéis importantes como habitats de reprodução e alimentação para triatomíneos silvestres, vetores da doença de Chagas. A ocorrência de triatomíneos em palmeiras peridomiciliares pode aumentar o risco de invasão desses insetos em domicílios e deve ser considerada para desenvolver estratégias de prevenção. Com objetivo de investigar a infestação de palmeiras por triatomíneos no Estado da Bahia e determinar a infecção natural desses insetos por Trypanosoma spp., foram amostradas 183 palmeiras em 12 municípios entre 2006 e 2011 utilizando captura manual e/ou armadilhas adesivas iscadas com camundongos. Os triatomíneos foram detectados em 79 palmeiras (43% das espécies Copernicia prunifera (Mart. Becc., Mauritia flexuosa L. e Attalea spp. (Attalea burretiana Bondar ou Attalea salvadorensis Glassman. Em outras espécies de palmeiras (Syagrus coronata (Mill H.E. Moore, Attalea funifera Mart ex. Spreng e Elaeis guineensis Jacq não foram detectados triatomíneos. Rhodnius neglectus Lent, Triatoma sordida (Stål, e Triatoma pseudomaculata Corrêa & Espínola ocorreram em C. prunifera ao longo do rio São Francisco. No extremo oeste da Bahia, R. neglectus e Psammolestes tertius Lent & Jurberg foram detectados em M. flexuosa, enquanto Triatoma tibiamaculata (Pinto ocorreu em Attalea sp. em áreas urbanas de Salvador. No total, 180 triatomíneos foram capturados, principalmente R. neglectus. A maior taxa de infecção natural (61% foi observada em T. tibiamaculata. Os resultados indicam que pelo menos três espécies de palmeiras são habitats favoráveis para triatomíneos no estado da Bahia e ocorrem no ambiente peridomiciliar, o que pode aumentar a probabilidade de invasão de triatomíneos nas casas.

  14. <em>Trichoderma harzianumem> como promotor del crecimiento vegetal del maracuyá (<em>Passiflora edulisem> var. <em>flavicarpa> Degener

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    Cubillos-Hinojosa Juan

    2009-04-01

    Full Text Available

    Se realizó un experimento en condiciones de laboratorio e invernadero, con el propósito de evaluar el efecto de la cepa nativa TCN-014 y la cepa comercial TCC-005 de <em>Trichoderma harzianumem> sobre la germinación y el crecimiento temprano del maracuyá. Se adecuaron inóculos de 104, 106 y 108 conidias/mL para cada cepa y se aplicaron a semillas de maracuyá; se evaluó el número de semillas germinadas durante 15 días; se calculó el porcentaje de germinación, el índice de velocidad de germinación y el tiempo medio de germinación. Posteriormente las semillas germinadas se llevaron a condiciones de invernadero y transcurridos dos meses se midió la altura de las plántulas, el grosor del tallo, el número de hojas, la longitud de la raíz y el peso seco total. Todos los tratamientos estimularon la germinación de las semillas y el desarrollo de las plántulas; sin embargo la cepa nativa en concentraciones 106 y 108 conidias/mL mostró resultados superiores frente a la cepa comercial. Los resultados sugieren una acción efectiva de <em>T. harzianumem> como promotor de crecimiento vegetal, mostrando que tiene potencial para la elaboración de un bioproducto útil para el manejo ecológico del cultivo de maracuyá.

  15. The Damage Capacity of Mahanarva spectabilis (Distant, 1909 (Hemiptera: Cercopidae Adults on Brachiaria ruziziensis Pasture

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    Tiago Teixeira Resende

    2013-01-01

    Full Text Available The aim of this study was to determine the damage caused by adult Mahanarva spectabilis (Distant, 1909 (Hemiptera: Cercopidae on Brachiaria ruziziensis (Germain & Evard under field conditions. A total of 0, 4, 8, 12, or 16 M. spectabilis adults per plot were maintained for 6 days. Thereafter, the insects were removed from the plant, and the following parameters were evaluated: chlorophyll content, damage score, dry as well as fresh weights, percentage of shoots’ dry matter, and the forage’s ability to regrow. The chlorophyll content was significantly reduced; the damage score and percentage of dry matter in plants increased depending on the increased insect infestation density after 6 days of exposure. In contrast, no change was observed on the B. ruziziensis fresh and dry weights as well as the regrowth capacity depending on the M. spectabilis infestation densities. Attacks by 8 adult M. spectabilis per clump of B. ruziziensis with an average of 80 tillers for 6 days were sufficient to reduce the chlorophyll content and the functional plant loss index. This density can be a reference for spittlebug integrated management in Brachiaria.

  16. Effect of β,β-Dimethylacrylshikonin on Inhibition of Human Colorectal Cancer Cell Growth <em>in Vitro em>and <em>in Vivoem>

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    Ting Feng

    2012-07-01

    Full Text Available In traditional Chinese medicine, shikonin and its derivatives, has been used in East Asia for several years for the prevention and treatment of several diseases, including cancer. We previously identified that β,β-dimethylacrylshikonin (DA could inhibit hepatocellular carcinoma growth. In the present study, we investigated the inhibitory effects of DA on human colorectal cancer (CRC cell line HCT-116 <em>in vitroem> and <em>in vivoem>. A viability assay showed that DA could inhibit tumor cell growth in a time- and dose-dependent manner. Flow cytometry showed that DA blocks the cell cycle at G0/G1 phase. Western blotting results demonstrated that the induction of apoptosis by DA correlated with the induction of pro-apoptotic proteins Bax, and Bid, and a decrease in the expression of anti-apoptotic proteins Bcl-2 and Bcl-xl. Furthermore, treatment of HCT-116 bearing nude mice with DA significantly retarded the growth of xenografts. Consistent with the results <em>in vitroem>, the DA-mediated suppression of HCT-116 xenografts correlated with Bax and Bcl-2. Taken together, these results suggest that DA could be a novel and promising approach to the treatment of CRC.

  17. New cost-effective bioconversion process of palm kernel cake into bioinsecticides based on Beauveria bassiana and Isaria javanica.

    Science.gov (United States)

    do Nascimento Silva, Jaqueline; Mascarin, Gabriel Moura; Dos Santos Gomes, Isabel Cristina; Tinôco, Ricardo Salles; Quintela, Eliane Dias; Dos Reis Castilho, Leda; Freire, Denise Maria Guimarães

    2018-03-01

    The present study aimed to add value to palm oil by-products as substrates to efficiently produce conidia of Beauveria bassiana and Isaria javanica (Hypocreales: Cordycipitaceae) for biological control of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae), through a solid-state fermentation process using palm kernel cake and palm fiber as nutrient source and solid matrix, respectively. The optimum culture conditions yielded high concentrations of viable conidia after air-drying, when the fungi were grown on palm kernel cake (B. bassiana 7.65 × 10 9 and I. javanica 2.91 × 10 9  conidia g -1 dry substrate) after 6 days under optimal growth conditions set to 60% substrate moisture and 32 °C. Both fungal strains exhibited high efficacy against third-instar whitefly nymphs, inducing mortality up to 62.9 and 56.6% by B. bassiana and I. javanica, respectively, assessed after 9 days post-application in a screenhouse. Furthermore, we noted that insect mortality was strongly correlated with high atmospheric moisture, while B. bassiana appeared to require shorter accumulative hours under high moisture to kill whitefly nymphs compared to I. javanica. Our results underpin a feasible and cost-effective mass production method for aerial conidia, using palm kernel as the main substrate in order to produce efficacious fungal bioinsecticides against an invasive whitefly species in Brazil. Finally, our fermentation process may offer a sustainable and cost-effective means to produce eco-friendly mycoinsecticides, using an abundant agro-industrial by-product from Brazil that will ultimately assist in the integrated management of agricultural insect pests.

  18. Enrichment and Purification of Syringin, Eleutheroside E and Isofraxidin from <em>Acanthopanax> <em>senticosus> by Macroporous Resin

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    Yuangang Zu

    2012-07-01

    Full Text Available In order to screen a suitable resin for the preparative simultaneous separation and purification of syringin, eleutheroside E and isofraxidin from <em>Acanthopanax> <em>senticosus>, the adsorption and desorption properties of 17 widely used commercial macroporous resins were evaluated. According to our results, HPD100C, which adsorbs by the molecular tiers model, was the best macroporous resin, offering higher adsorption and desorption capacities and higher adsorption speed for syringin, eleutheroside E and isofraxidin than other resins. Dynamic adsorption and desorption tests were carried out to optimize the process parameters. The optimal conditions were as follows: for adsorption, processing volume: 24 BV, flow rate: 2 BV/h; for desorption, ethanol–water solution: 60:40 (v/v, eluent volume: 4 BV, flow rate: 3 BV/h. Under the above conditions, the contents of syringin, eleutheroside E and isofraxidin increased 174-fold, 20-fold and 5-fold and their recoveries were 80.93%, 93.97% and 93.79%, respectively.

  19. Training for Defense? From Stochastic Traits to Synchrony in Giant Honey Bees (<em>Apis dorsataem>

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    Gerald Kastberger

    2012-08-01

    Full Text Available In Giant Honey Bees, abdomen flipping happens in a variety of contexts. It can be either synchronous or cascaded, such as in the collective defense traits of shimmering and rearing-up, or it can happen as single-agent behavior. Abdomen flipping is also involved in flickering behavior, which occurs regularly under quiescent colony state displaying singular or collective traits, with stochastic, and (semi- synchronized properties. It presumably acts via visual, mechanoceptive, and pheromonal pathways and its goals are still unknown. This study questions whether flickering is preliminary to shimmering which is subject of the <em>fs em>(flickering-shimmering-transition> hypothesis? We tested the respective prediction that trigger sites (<em>ts> at the nest surface (where shimmering waves had been generated show higher flickering activity than the alternative non-trigger sites (<em>nts>. We measured the flickering activity of <em>ts>- and <em>nts>-surface bees from two experimental nests, before and after the colony had been aroused by a dummy wasp. Arousal increased rate and intensity of the flickering activity of both <em>ts>- and <em>nts> cohorts (P < 0.05, whereby the flickering intensity of <em>ts>-bees were higher than that of <em>nts>-bees (P < 0.05. Under arousal, the colonies also increased the number of flickering-active <em>ts>- and <em>nts>-cohorts (P < 0.05. This provides evidence that cohorts which are specialist at launching shimmering waves are found across the quiescent nest zone. It also proves that arousal may reinforce the responsiveness of quiescent curtain bees for participating in shimmering, practically by recruiting additional trigger site bees for expanding repetition of rate and intensity of shimmering waves. This finding confirms the <em>fs-transition> hypothesis and constitutes evidence that flickering is part of a basal colony-intrinsic information system

  20. Molecular markers reveal infestation dynamics of the bed bug (Hemiptera: Cimicidae) within apartment buildings.

    Science.gov (United States)

    Booth, Warren; Saenz, Virna L; Santangelo, Richard G; Wang, Changlu; Schal, Coby; Vargo, Edward L

    2012-05-01

    The bed bug, Cimex lectularius L. (Hemiptera: Cimicidae), has experienced an extraordinary global resurgence in recent years, the reasons for which remain poorly understood. Once considered a pest of lower socioeconomic classes, bed bugs are now found extensively across all residential settings, with widespread infestations established in multiapartment buildings. Within such buildings, understanding the population genetic structure and patterns of dispersal may prove critical to the development of effective control strategies. Here, we describe the development of 24 high-resolution microsatellite markers through next generation 454 pyrosequencing and their application to elucidate infestation dynamics within three multistory apartment buildings in the United States. Results reveal contrasting characteristics potentially representative of geographic or locale differences. In Raleigh, NC, an infestation within an apartment building seemed to have started from a single introduction followed by extensive spread. In Jersey City, NJ, two or more introductions followed by spread are evident in two buildings. Populations within single apartments in all buildings were characterized by high levels of relatedness and low levels of diversity, indicative of foundation from small, genetically depauperate propagules. Regardless of the number of unique introductions, genetic data indicate that spread within buildings is extensive, supporting both active and human-mediated dispersal within and between adjacent rooms or apartments spanning multiple floors.

  1. Exposure to Guava Affects Citrus Olfactory Cues and Attractiveness to Diaphorina citri (Hemiptera: Psyllidae).

    Science.gov (United States)

    Barman, Jagadish Chandra; Campbell, Stuart A; Zeng, Xinnian

    2016-06-01

    Intercropping can reduce agricultural pest incidence, and represents an important sustainable alternative to conventional pest control methods. Understanding the ecological mechanisms for intercropping could help optimize its use, particularly in tropical systems which present a large number of intercropping possibilities. Citrus is threatened worldwide by greening disease (huanglongbing, HLB) vectored by the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae). Control of HLB and citrus psyllid can be partially achieved through intercropping with guava, Psidium guajava L., but the mechanisms remain unclear. We tested the hypothesis that guava olfactory cues affect psyllid behavior by altering the attractiveness of citrus through plant-plant interactions. In choice and no-choice cage experiments, psyllid settlement was reduced on citrus shoots that had been exposed to guava shoot odors for at least 2 h. In Y-tube olfactometer experiments, psyllids oriented to odors of unexposed, compared with guava-exposed, citrus shoots. These behavioral results indicate that a mechanism for the success of guava intercropping for sustainable, ecological disease management may be the indirect effect of guava on citrus attractiveness. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  2. Understanding trophic interactions of Orius spp. (Hemiptera: Anthocoridae) in lettuce crops by molecular methods.

    Science.gov (United States)

    Gomez-Polo, Priscila; Alomar, Oscar; Castañé, Cristina; Aznar-Fernández, Thaïs; Lundgren, Jonathan G; Piñol, Josep; Agustí, Nuria

    2016-02-01

    The aphid Nasonovia ribisnigri (Mosley) (Hemiptera: Aphididae) and the thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) are common pests in Mediterranean lettuce crops, where Orius spp. are common generalist predators. Predation by Orius spp. was studied in a lettuce plot by conventional polymerase chain reaction (PCR) and real-time PCR analyses using specific primers of both main pests. Also, high-throughput sequencing was used to have a wider approach of the diet of these predators in natural field conditions. Molecular analyses indicated a higher predation on N. ribisnigri in spring and on F. occidentalis in summer. Predation on alternative prey, like Collembola, was also found in both seasons. Real-time PCR was more sensitive than conventional PCR in showing the target trophic links, whereas high-throughput sequencing revealed predation on other natural enemies - intraguild predation (IGP), showing other trophic interactions of Orius majusculus within the studied ecosystem. This study gives important information about the trophic relationships present in Mediterranean lettuce crops in different periods of the year. The detected predation by Orius spp. on alternative prey, as well as on other natural enemies, should be further investigated to clarify whether it adds or detracts to the biological control of N. ribisnigri and F. occidentalis. © 2015 Society of Chemical Industry.

  3. Complete BWR--EM LOCA analysis using the WRAP--EM system

    International Nuclear Information System (INIS)

    Beckmeyer, R.R.; Gregory, M.V.; Buckner, M.R.

    1979-01-01

    The Water Reactor Analysis Package, Evaluation Model (WRAP--EM), provides a complete analysis of postulated loss-of-coolant accidents (LOCA's) in light--water nuclear power reactors. The system is being developed at the Savannah River Laboratory (SRL) for use by the Nuclear Regulatory Commission (NRC) to interpret and evaluate reactor vendor, evaluation model (EM) analyses. The initial version of the WRAP--EM system for analysis of boiling water reactors (BWR's) is operational. To demonstrate the complete capability of the WRAP--BWR--EM system, a LOCA analysis has been performed for the Hope Creek Plant

  4. Low levels of mitochondrial DNA and symbiont diversity in the worldwide agricultural pest, the greenhouse whitefly Trialeurodes vaporariorum (Hemiptera: Aleyrodidae).

    Science.gov (United States)

    Kapantaidaki, Despoina E; Ovčarenko, Irina; Fytrou, Natasa; Knott, K Emily; Bourtzis, Kostas; Tsagkarakou, Anastasia

    2015-01-01

    Trialeurodes vaporariorum, the greenhouse whitefly, is a cosmopolitan agricultural pest. Little is known about the genetic diversity of T. vaporariorum and the bacterial symbionts associated with this species. Here, we undertook a large phylogeographic study by investigating both the mitochondrial (mt) diversity and the infection status of 38 T. vaporariorum collections from 18 countries around the world. Genetic diversity of T. vaporariorum was studied by analyzing sequence data from the mt cytochrome oxidase I, cytochrome b, and NADH dehydrogenase subunit 5 genes. Maximum-likelihood (ML) phylogeny reconstruction delineated 2 clades characterized by limited sequence divergence: one clade comprised samples only from the Northern hemisphere whereas the other comprised samples from a broader geographical range. The presence of secondary symbionts was determined by PCR using primers specific for Hamiltonella, Rickettsia, Arsenophonus, Cardinium, Wolbachia, and Fritschea. Most individuals examined harbored at least one secondary endosymbiont, and Arsenophonus was detected in almost all male and female individuals. Wolbachia was present at a much lower frequency, and Cardinium was detected in only a few individuals from Greece. Rickettsia, Hamiltonella, and Fritschea were not found. Additionally, we set out to further analyze Arsenophonus diversity by multilocus sequence typing analysis; however, the Arsenophonus sequences did not exhibit any polymorphism. Our results revealed remarkably low diversity in both mtDNA and symbionts in this worldwide agricultural pest, contrasting sharply with that of the ecologically similar Bemisia tabaci. © The American Genetic Association 2014. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  5. Is agriculture driving the diversification of the Bemisia tabaci species complex (Hemiptera: Sternorrhyncha: Aleyrodidae)?: Dating, diversification and biogeographic evidence revealed.

    Science.gov (United States)

    Boykin, Laura M; Bell, Charles D; Evans, Gregory; Small, Ian; De Barro, Paul J

    2013-10-18

    Humans and insect herbivores are competing for the same food crops and have been for thousands of years. Despite considerable advances in crop pest management, losses due to insects remain considerable. The global homogenisation of agriculture has supported the range expansion of numerous insect pests and has been driven in part by human-assisted dispersal supported through rapid global trade and low-cost air passenger transport. One of these pests, is the whitefly, Bemisia tabaci, a cryptic species complex that contains some of the world's most damaging pests of agriculture. The complex shows considerable genetic diversity and strong phylogeographic relationships. One consequence of the considerable impact that members of the B. tabaci complex have on agriculture, is the view that human activity, particularly in relation to agricultural practices, such as use of insecticides, has driven the diversification found within the species complex. This has been particularly so in the case of two members of the complex, Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED), which have become globally distributed invasive species. An alternative hypothesis is that diversification is due to paleogeographic and paleoclimatological changes. The idea that human activity is driving speciation within the B. tabaci complex has never been tested, but the increased interest in fossil whiteflies and the growth in molecular data have enabled us to apply a relaxed molecular clock and so estimate divergence dates for the major lineages within the B. tabaci species complex. The divergence estimates do not support the view that human activity has been a major driver of diversification. Our analysis suggests that the major lineages within the complex arose approximately 60-30 mya and the highly invasive MED and MEAM1 split from the rest of the species complex around 12 mya well before the evolution of Homo sapiens and agriculture. Furthermore, the divergence dates coincide with a period of global diversification that occurred broadly across the plant and animal kingdoms and was most likely associated with major climatic and tectonic events.

  6. First report and differential colonization of Passiflora species by the B biotype of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in Brazil

    International Nuclear Information System (INIS)

    Nunes, Endson S.; Vieira, Maria L.C.; Lourencao, Andre L.; Piedade, Sonia M.S.

    2008-01-01

    This note is the first report of Bemisia tabaci (Gennadius) biotype B colonizing passionvine in Brazil. We examined the colonization of nine Passiflora species by a wild B type population under greenhouse conditions. P. amethystina Mikan was the most preferred species for oviposition and colonization, whereas P. suberosa L., P. coriacea Juss. and two commercially cultivated species, P. alata Curtis and P. edulis Sims f. flavicarpa Degener, were mostly uncolonised. P. morifolia Mast., P. cincinnata Mast., P. foetida L. and P. caerulea L. showed intermediate levels of colonization. Such differential colonization might suggest some degree of resistance by certain Passiflora species or oviposition preference by B. tabaci. (author)

  7. Estudio de la resistencia a Bemisia tabaci (Gennadius (Hemiptera: Aleyrodidae en germoplasma cultivado y silvestre de tomate (Solanum lycopersicum L.

    Directory of Open Access Journals (Sweden)

    Yeimy García Valencia

    2013-12-01

    Full Text Available En la Universidad Nacional de Colombia sede Palmira, en condiciones de campo y casa de malla, se evaluaron por preferencia para oviposición y número de ninfas de mosca blanca (Bemisia tabaci (Gennadius varias accesiones silvestres y poblaciones segregantes de tomate resistentes al pasador del fruto, Neoleucinodes elegantalis (Guenée. Se utilizó un diseño de bloques completos al azar con cuatro repeticiones, donde se evaluaron como variables el número de huevos y de ninfas del insecto por hoja. En cada observación se incluyeron tres hojas del tercio superior, medio e inferior del tallo de la planta. Las poblaciones de mosca blanca fueron muy bajas en los dos primeros ciclos de cultivo (en campo debido, probablemente, a las altas precipitaciones. Estas condiciones no permitieron una selección de materiales resistentes o susceptibles a este insecto. En el tercer ciclo de cultivo, bajo condiciones de casa de malla, no se encontraron diferencias significativas entre los retrocruzamientos respecto al testigo susceptible para la variable preferencia por oviposición. El genotipo 4212P4 presentó el menor número de ninfas, mostrando que tiene posiblemente un mecanismo que le confiere menor atracción para B. tabaci. Se encontró que los genotipos menos preferidos para oviposición y con menor número de ninfas fueron PI 134417, PI 134418 y PI 126449 (Solanum habrochaites var. Glabratum S. Knapp y D. M Spooneer.

  8. Status of Resistance of Bemisia tabaci (Hemiptera: Aleyrodidae) to Neonicotinoids in Iran and Detoxification by Cytochrome P450-Dependent Monooxygenases.

    Science.gov (United States)

    Basij, M; Talebi, K; Ghadamyari, M; Hosseininaveh, V; Salami, S A

    2017-02-01

    Nine Bemisia tabaci (Gennadius) populations were collected from different regions of Iran. In all nine populations, only one biotype (B biotype) was detected. Susceptibilities of these populations to imidacloprid and acetamiprid were assayed. The lethal concentration 50 values (LC 50 ) for different populations showed a significant discrepancy in the susceptibility of B. tabaci to imidacloprid (3.76 to 772.06 mg l -1 ) and acetamiprid (4.96 to 865 mg l -1 ). The resistance ratio of the populations ranged from 9.72 to 205.20 for imidacloprid and 6.38 to 174.57 for acetamiprid. The synergistic effects of piperonylbutoxide (PBO) and S,S,S-tributylphosphorotrithioate (DEF) were evaluated for the susceptible (RF) and resistant (JR) populations for the determination of the involvement of cytochrome P450-dependent monooxygenase and carboxylesterase, respectively, in their resistance mechanisms. The results showed that PBO overcame the resistance of the JR population to both imidacloprid and acetamiprid, with synergistic ratios of 72.7 and 106.9, respectively. Carboxylesterase, glutathione S-transferase and cytochrome P450-dependent monooxygenase were studied biochemically, for the purpose of measuring the activity of the metabolizing enzymes in order to determine which enzymes are directly involved in neonicotinoid resistance. There was an increase in the activity of cytochrome P450-dependent monooxygenase up to 17-fold in the resistant JR population (RR = 205.20). The most plausible activity of cytochrome P450-dependent monooxygenase correlated with the resistances of imidacloprid and acetamiprid, and this suggests that cytochrome P450-dependent monooxygenase is the only enzyme system responsible for neonicotinoid resistance in the nine populations of B. tabaci.

  9. First report and differential colonization of Passiflora species by the B biotype of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Nunes, Endson S.; Vieira, Maria L.C. [Escola Superior de Agricultura Luiz de Queiroz (ESALQ-USP), Piracicaba, SP (Brazil). Dept. de Genetica]. E-mail: esnunes@carpa.ciagri.usp.br; mlcvieir@esalq.usp.br; Brown, Judith K. [University of Arizona, Tucson, AZ (United States). Dept. of Plant Sciences]. E-mail: jbrown@Ag.arizona.edu; Moreira, Adriana G.; Rezende, Jorge A.M. [Escola Superior de Agricultura Luiz de Queiroz (ESALQ-USP), Piracicaba, SP (Brazil). Dept. de Entomologia, Fitopatologia e Zoologia Agricola]. E-mails: agmoreir@esalq.usp.br; amrezen@esalq.usp.br; Watson, Gillian [California Dept. of Food and Agriculture, Sacramento, CA (United States)]. E-mail: gwatson@cdfa.ca.gov; Lourencao, Andre L. [Instituto Agronomico, Campinas, SP (Brazil). Centro de Pesquisa e Desenvolvimento de Fitossanidade]. E-mail: andre@iac.sp.gov.br; Piedade, Sonia M.S. [Escola Superior de Agricultura Luiz de Queiroz (ESALQ-USP), Piracicaba, SP (Brazil). Dept. de Ciencias Exatas]. E-mail: jsoniamsp@esalq.usp.br

    2008-11-15

    This note is the first report of Bemisia tabaci (Gennadius) biotype B colonizing passionvine in Brazil. We examined the colonization of nine Passiflora species by a wild B type population under greenhouse conditions. P. amethystina Mikan was the most preferred species for oviposition and colonization, whereas P. suberosa L., P. coriacea Juss. and two commercially cultivated species, P. alata Curtis and P. edulis Sims f. flavicarpa Degener, were mostly uncolonised. P. morifolia Mast., P. cincinnata Mast., P. foetida L. and P. caerulea L. showed intermediate levels of colonization. Such differential colonization might suggest some degree of resistance by certain Passiflora species or oviposition preference by B. tabaci. (author)

  10. Understanding biological control of greenhouse whitefly with the parasitoid Encarsia formosa : from individual behaviour to population dynamics

    NARCIS (Netherlands)

    Roermund, van H.J.W.

    1995-01-01

    The greenhouse whitefly, Trialeurodes vaporariorum (Westwood) (Homoptera, Aleyrodidae), is a very common, highly polyphagous pest insect all over the world. Biological control of whiteflies with the parasitoid Encarsia formosa Gahan (Hymenoptera, Aphelinidae) was already applied in the 1920s in

  11. Supercritical Fluid Extraction of <em>Eucalyptus globulusem> Bark—A Promising Approach for Triterpenoid Production<em>>

    Directory of Open Access Journals (Sweden)

    Carlos M. Silva

    2012-06-01

    Full Text Available Eucalyptus bark contains significant amounts of triterpenoids with demonstrated bioactivity, namely triterpenic acids and their acetyl derivatives (ursolic, betulinic, oleanolic, betulonic, 3-acetylursolic, and 3-acetyloleanolic acids. In this work, the supercritical fluid extraction (SFE of <em>Eucalyptus> <em>globulus> deciduous bark was carried out with pure and modified carbon dioxide to recover this fraction, and the results were compared with those obtained by Soxhlet extraction with dichloromethane. The effects of pressure (100–200 bar, co-solvent (ethanol content (0, 5 and 8% wt, and multistep operation were studied in order to evaluate the applicability of SFE for their selective and efficient production. The individual extraction curves of the main families of compounds were measured, and the extracts analyzed by GC-MS. Results pointed out the influence of pressure and the important role played by the co-solvent. Ethanol can be used with advantage, since its effect is more important than increasing pressure by several tens of bar. At 160 bar and 40 °C, the introduction of 8% (wt of ethanol greatly improves the yield of triterpenoids more than threefold.

  12. <em>Helicobacter pyloriem> Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation

    Directory of Open Access Journals (Sweden)

    Hsueh-Fen Juan

    2012-08-01

    Full Text Available <em>Helicobacter pyloriem> (<em>H. pyloriem>, the human stomach pathogen, lives on the inner surface of the stomach and causes chronic gastritis, peptic ulcer, and gastric cancer. Plasma membrane repair response is a matter of life and death for human cells against physical and biological damage. We here test the hypothesis that <em>H. pyloriem> also causes plasma membrane disruption injury, and that not only a membrane repair response but also a cell proliferation response are thereby activated. Vacuolating cytotoxin A (VacA and cytotoxin-associated gene A (CagA have been considered to be major <em>H. pyloriem> virulence factors. Gastric cancer cells were infected with <em>H. pyloriem> wild type (vacA+/cagA+, single mutant (ΔvacA or ΔcagA or double mutant (ΔvacA/ΔcagA strains and plasma membrane disruption events and consequent activation of membrane repair components monitored. <em>H. pyloriem> disrupts the host cell plasma membrane, allowing localized dye and extracellular Ca2+ influx. Ca2+-triggered members of the annexin family, A1 and A4, translocate, in response to injury, to the plasma membrane, and cell surface expression of an exocytotic maker of repair, LAMP-2, increases. Additional forms of plasma membrane disruption, unrelated to <em>H.> pylori em>exposure, also promote host cell proliferation. We propose that <em>H. pyloriem> activation of a plasma membrane repair is pro-proliferative. This study might therefore provide new insight into potential mechanisms of <em>H. pyloriem>-induced gastric carcinogenesis.

  13. Laboratory Evaluation of Different Insecticides against Hibiscus Mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae

    Directory of Open Access Journals (Sweden)

    Samman Fatima

    2016-01-01

    Full Text Available Hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae, is the major pest of many vegetables, fruits, crops, and ornamental plants causing losses to the farmers and its control has been an issue of significance in the pest management. This study was aimed at evaluating different concentrations (0.06%, 0.1%, and 0.14% of Telsta, Advantage, Talstar, Imidacloprid, and their mixtures against hibiscus mealybug in the Laboratory of Systematics and Pest Management at University of Gujrat, Pakistan. The toxic effect was evaluated in the laboratory bioassay after 24 and 48 h of the application of insecticides. The highest mortality (95.83% was shown by Talstar and Talstar + Imidacloprid at the concentration of 0.14% after 48 h followed by Advantage + Talstar with 87.50% mortality at 0.14% concentration after 48 h of application. The study also showed that the least effective treatment observed was Advantage + Telsta with no mortality after 24 h and 25% mortality after 48 h at 0.14% concentration. The study revealed that the concentration 0.14% was highly effective in lowering the mealybug population and insecticide mixtures were effective in reducing mealybug density. The study emphasizes the use of such insecticide mixtures to develop better management strategy for mealybug populations attacking ornamental plants. However effects of such insecticide mixtures on other organisms and biological control agents should be checked under field conditions.

  14. The Hemiptera (Insecta) of Canada: Constructing a Reference Library of DNA Barcodes

    Science.gov (United States)

    Gwiazdowski, Rodger A.; Foottit, Robert G.; Maw, H. Eric L.; Hebert, Paul D. N.

    2015-01-01

    DNA barcode reference libraries linked to voucher specimens create new opportunities for high-throughput identification and taxonomic re-evaluations. This study provides a DNA barcode library for about 45% of the recognized species of Canadian Hemiptera, and the publically available R workflow used for its generation. The current library is based on the analysis of 20,851 specimens including 1849 species belonging to 628 genera and 64 families. These individuals were assigned to 1867 Barcode Index Numbers (BINs), sequence clusters that often coincide with species recognized through prior taxonomy. Museum collections were a key source for identified specimens, but we also employed high-throughput collection methods that generated large numbers of unidentified specimens. Many of these specimens represented novel BINs that were subsequently identified by taxonomists, adding barcode coverage for additional species. Our analyses based on both approaches includes 94 species not listed in the most recent Canadian checklist, representing a potential 3% increase in the fauna. We discuss the development of our workflow in the context of prior DNA barcode library construction projects, emphasizing the importance of delineating a set of reference specimens to aid investigations in cases of nomenclatural and DNA barcode discordance. The identification for each specimen in the reference set can be annotated on the Barcode of Life Data System (BOLD), allowing experts to highlight questionable identifications; annotations can be added by any registered user of BOLD, and instructions for this are provided. PMID:25923328

  15. Molecular and Morphological Identification of Mealybug Species (Hemiptera: Pseudococcidae) in Brazilian Vineyards

    Science.gov (United States)

    Pacheco da Silva, Vitor C.; Bertin, Aline; Blin, Aurélie; Germain, Jean-François; Bernardi, Daniel; Rignol, Guylène; Botton, Marcos; Malausa, Thibaut

    2014-01-01

    Mealybugs (Hemiptera: Pseudococcidae) are pests constraining the international trade of Brazilian table grapes. They damage grapes by transmitting viruses and toxins, causing defoliation, chlorosis, and vigor losses and favoring the development of sooty mold. Difficulties in mealybug identification remain an obstacle to the adequate management of these pests. In this study, our primary aim was to identify the principal mealybug species infesting the major table grape-producing regions in Brazil, by morphological and molecular characterization. Our secondary aim was to develop a rapid identification kit based on species-specific Polymerase Chain Reactions, to facilitate the routine identification of the most common pest species. We surveyed 40 sites infested with mealybugs and identified 17 species: Dysmicoccus brevipes (Cockerell), Dysmicoccus sylvarum Williams and Granara de Willink, Dysmicoccus texensis (Tinsley), Ferrisia cristinae Kaydan and Gullan, Ferrisia meridionalis Williams, Ferrisia terani Williams and Granara de Willink, Phenacoccus baccharidis Williams, Phenacoccus parvus Morrison, Phenacoccus solenopsis Tinsley, Planococcus citri (Risso), Pseudococcus viburni (Signoret), Pseudococcus cryptus Hempel, four taxa closely related each of to Pseudococcus viburni, Pseudococcus sociabilis Hambleton, Pseudococcus maritimus (Ehrhorn) and Pseudococcus meridionalis Prado, and one specimen from the genus Pseudococcus Westwood. The PCR method developed effectively identified five mealybug species of economic interest on grape in Brazil: D. brevipes, Pl. citri, Ps. viburni, Ph. solenopsis and Planococcus ficus (Signoret). Nevertheless, it is not possible to assure that this procedure is reliable for taxa that have not been sampled already and might be very closely related to the target species. PMID:25062012

  16. Degree Day Requirements for Kudzu Bug (Hemiptera: Plataspidae), a Pest of Soybeans.

    Science.gov (United States)

    Grant, Jessica I; Lamp, William O

    2018-04-02

    Understanding the phenology of a new potential pest is fundamental for the development of a management program. Megacopta cribraria Fabricius (Hemiptera: Plataspidae), kudzu bug, is a pest of soybeans first detected in the United States in 2009 and in Maryland in 2013. We observed the phenology of kudzu bug life stages in Maryland, created a Celsius degree-day (CDD) model for development, and characterized the difference between microhabitat and ambient temperatures of both kudzu, Pueraria montana (Lour.) Merr. (Fabales: Fabaceae) and soybeans, Glycine max (L.) Merrill (Fabales: Fabaceae). In 2014, low population numbers yielded limited resolution from field phenology observations. We observed kudzu bug populations persisting within Maryland; but between 2013 and 2016, populations were low compared to populations in the southeastern United States. Based on the degree-day model, kudzu bug eggs require 80 CDD at a minimum temperature of 14°C to hatch. Nymphs require 545 CDD with a minimum temperature of 16°C for development. The CDD model matches field observations when factoring a biofix date of April 1 and a minimum preoviposition period of 17 d. The model suggests two full generations per year in Maryland. Standard air temperature monitors do not affect model predictions for pest management, as microhabitat temperature differences did not show a clear trend between kudzu and soybeans. Ultimately, producers can predict the timing of kudzu bug life stages with the CDD model for the use of timing management plans in soybean fields.

  17. Molecular Characterization and Expression Profiles of Polygalacturonase Genes in Apolygus lucorum (Hemiptera: Miridae.

    Directory of Open Access Journals (Sweden)

    Lili Zhang

    Full Text Available Polygalacturonase (PG is an enzyme in the salivary glands of piercing-sucking mirid bugs (Hemiptera: Miridae that plays a key role in plant feeding and injury. By constructing a full-length cDNA library, we cloned and characterized 14 PG genes from the salivary glands of Apolygus lucorum, a pestiferous mirid bug in cotton, fruit trees and other crops in China. BLAST search analysis showed that the amino acid sequences deduced from transcripts of the PG genes were closely related to PGs from other mirid bugs. Phylogenetic analysis showed that the PGs of mirid bugs had six main branches, PG1-PG6 (Genbank accession numbers: KF881899~KF881912. We investigated the mRNA expression patterns of the A. lucorum PG genes using real-time PCR. All 14 PGs were expressed significantly higher in the salivary glands than in other tissues (head, thorax, abdomen, leg and wing. For eggs and nymphs, the expression levels of these PGs were much higher in the 5th instar stage than in the egg, and 1st and 3rd instar stages. The PG expression levels in 1-day-old adults were very low, and increased in 5, 20 and 30-day-old adults. Additionally, PG expression levels were generally similar between males and females. The possible physiological functions of PGs in A. lucorum were discussed.

  18. Effects of gamma irradiation on different stages of mealybug Dysmicoccus neobrevipes (Hemiptera: Pseudococcidae)

    International Nuclear Information System (INIS)

    The, Doan Thi; Khanh, Nguyen Thuy; Lang, Vo Thi Kim; Van Chung, Cao; An, Tran Thi Thien; Thi, Nguyen Hoang Hanh

    2012-01-01

    Utilization of phytosanitary irradiation as a potential treatment to disinfest agricultural commodities in trade has expanded rapidly in the recent years. Cobalt-60 gamma ray target doses of 100, 150, 200 and 250 Gy were used to irradiate immatures and adults of Dysmicoccus neobrevipes (Beardsley) (Hemiptera: Pseudococcidae) infesting dragon fruits to find the most tolerant stage and the most optimal dose range for quarantine treatment. In general, irradiation affected significantly all life stages of D. neobrevipes mortality and adult reproduction. The pattern of tolerance to irradiation in D. neobrevipes was 1st instars<2nd instars<3rd instars< adults, in which the adult is the most tolerant stage. Based on obtained results after 21 days of irradiation, predicted doses for 100% mortality of each different development stage in the above mentioned pattern were 224.6, 241.3, 330.9 and 581.5 Gy, respectively. No survived female adult produced offspring at 200 and 250 Gy. Dose range between 200 and 250 Gy could be efficient to prevent the reproduction of this mealybug. - Highlights: → Gamma Co-60 irradiation as a potential phytosanitary for quarantine treatments. → Reproduction ability of D. neobrevipes has been efficiently inhibited at low dose. → Pattern of tolerance to irradiation was 1st<2nd<3rd instars< adults. → Doses from 200 to 250 Gy could be efficient to prevent the reproduction of mealybug.

  19. Effects of gamma irradiation on different stages of mealybug Dysmicoccus neobrevipes (Hemiptera: Pseudococcidae)

    Energy Technology Data Exchange (ETDEWEB)

    The, Doan Thi, E-mail: doanthithe@yahoo.com [Research and Development Center for Radiation Technology, Vietnam Atomic Energy Institute, 202A Street 11, Linh Xuan Ward, Thu Duc District, Ho Chi Minh City (Viet Nam); Khanh, Nguyen Thuy; Lang, Vo Thi Kim; Van Chung, Cao [Research and Development Center for Radiation Technology, Vietnam Atomic Energy Institute, 202A Street 11, Linh Xuan Ward, Thu Duc District, Ho Chi Minh City (Viet Nam); An, Tran Thi Thien; Thi, Nguyen Hoang Hanh [Agriculture and Forestry University, Linh Trung Ward, Thu Duc District, Ho Chi Minh City (Viet Nam)

    2012-01-15

    Utilization of phytosanitary irradiation as a potential treatment to disinfest agricultural commodities in trade has expanded rapidly in the recent years. Cobalt-60 gamma ray target doses of 100, 150, 200 and 250 Gy were used to irradiate immatures and adults of Dysmicoccus neobrevipes (Beardsley) (Hemiptera: Pseudococcidae) infesting dragon fruits to find the most tolerant stage and the most optimal dose range for quarantine treatment. In general, irradiation affected significantly all life stages of D. neobrevipes mortality and adult reproduction. The pattern of tolerance to irradiation in D. neobrevipes was 1st instars<2nd instars<3rd instars Gamma Co-60 irradiation as a potential phytosanitary for quarantine treatments. > Reproduction ability of D. neobrevipes has been efficiently inhibited at low dose. > Pattern of tolerance to irradiation was 1st<2nd<3rd instars Doses from 200 to 250 Gy could be efficient to prevent the reproduction of mealybug.

  20. NATURE AND LAW IN THE <em>INSTITUTAS> OF GAIO NATUREZA E DIREITO NAS <em>INSTITUTAS> DE GAIO

    Directory of Open Access Journals (Sweden)

    Alfredo Carlos Storck

    2011-09-01

    Full Text Available

    In a very famous passage of his <em>Institutes>, the ancient jurist Gaius had divided the Roman Private Law in two parts: the Civil Law and the Law of People. The former is the Law created by the Roman people, whereas the latter is the Law followed by every nation.

    Despite its classical origin, there is some disagreement among specialists about the right way to interpret this distinction. By the beginning of the 20th century, historians like Michel Villey had seen in this distinction the influence of Greek philosophy (Stoic but even Aristotelian among the Roman jurists. But Levy and more recently Kelly have challenged this view and proposed that, in the roman period, philosophers and jurists had completely distinct notions of nature and natural law. The aim of this paper is not to prove the exact influence of Greek philosophy among jurists, but only to suggest that it is not possible to give an interpretation of the classical texts without ascribing to them a theoretical notion of the natural law.

    Em uma famosa passagem de suas <em>Institutas>, o jurista romano Gaio dividiu o direito privado em duas partes: o direito civil e o direito dos povos. O primeiro é o direito criado pelo povo romano enquanto o segundo é o direito seguido por todas as nações. A despeito de sua origem clássica, os especialistas discordam acerca do modo de interpretar essa tese. No início do século XX, historiadores como Michel Villey viram na distinção sinais da influência da filosofia grega (estóica e mesmo aristotélica entre os juristas romanos. Posteriormente Levy e mais recentemente Kelly contestaram essa afirmação e defenderam que filósofos e juristas possuíam, no período clássico, noções de natureza e direito natural completamente distintas. O objetivo desse artigo não está em provar a exata influência da filosofia grega entre os juristas, mas apenas em sugerir que não é possível fornecer uma interpretação dos

  1. Local feeding specialization of the red fox (<em>Vulpes vulpesem> in response to eastern cottontail (<em>Sylvilagus floridanusem> introduction (NW Italy

    Directory of Open Access Journals (Sweden)

    Alessandro Balestrieri

    2006-03-01

    Full Text Available Abstract To appreciate the influence of the introduction of the Eastern cottontail (<em>Sylvilagus floridanusem> on the food habits of the red fox (Vulpes vulpes, between June 1998 and February 2000 fox diet was investigated by means of scat analysis (N=115 in a 250 ha wide Natural Reserve of NW Italy, and compared with data collected in the same area prior to cottontail colonization (1988-1989. Comparison included also the diet of badgers (<em>Meles melesem>, considered as potential competitors for food resources. Alien lagomorphs (mean percent volume, Vm% = 68% represented by far the most exploited resource, only three other food items reaching values of mean percent volume barely higher than 5%. Cottontails frequency of occurrence did not vary according either to season or to their reproductive cycle (II-IX vs. X-I, whilst diet niche breadth varied inversely proportional to the use of this key-resource. Overall fox trophic niche breadth varied from 0.64 in 1988-89 to 0.31 in 1998-00 (B, Levin’s index. These findings led us to consider the feeding habits of the fox in the study area as a result of local specialization of a typical generalist carnivore, according to the predictions of optimal foraging theory. No variation occurred in the badger niche breadth since cottontail introduction, whilst niche overlap between foxes and badgers decreased from 0.59 to 0.13 (O, Pianka’s index, possibly reducing competition for food in summer. Riassunto Specializzazione alimentare a livello locale della Volpe <em>Vulpes vulpesem> in risposta all’introduzione del Silvilago <em>Sylvilagus floridanusem> (Italia nord occidentale. Per valutare gli effetti dell’introduzione del Silvilago (<em>Sylvilagus floridanusem> sul comportamento alimentare della volpe (<em>Vulpes vulpesem>, nel periodo giugno 1998-febbraio 2000, la dieta del carnivoro è stata definita tramite l’analisi di 115

  2. New record of Helopeltis bradyi Waterhouse and Pachypeltis maesarum Kirkaldy (Hemiptera: Miridae on tea Camellia sinensis L.O. (Kuntze in southern India

    Directory of Open Access Journals (Sweden)

    Kodakkadan SRIKUMAR

    2017-03-01

    Full Text Available Helopeltis theivora commonly called as tea mosquito bug (TMB is considered as important pest of tea in South India. Extensive survey in tea plantations of Anamallais, recorded H. bradyi and Pachypeltis maesarum (Hemiptera: Miridae causing considerable damage and were registered for the first time on tea. Their occurrence was seen in association with H. theivora. There was considerable variation among these species in size of the circular rings formed by rostral piercing, indicating that these three species can be easily demarcated based on the observation of feeding punctures even in field condition. As the dosage and recommendations of insecticides against TMB in tea in south India was pertaining only to H. theivora the addition of H. bradyi and P. maesarum will improve the future pest management strategies.

  3. First report of Maconellicoccus hirsutus (Green, 1908) (Hemiptera: Coccoidea: Pseudococcidae) and the associated parasitoid Anagyrus kamali Moursi, 1948 (Hymenoptera: Encyrtidae), in Brazil.

    Science.gov (United States)

    Marsaro Júnior, A L; Peronti, A L B G; Penteado-Dias, A M; Morais, E G F; Pereira, P R V S

    2013-05-01

    The pink hibiscus mealybug (PHM), Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) and the associated hymenopterous parasitoid, Anagyrus kamali Moursi, 1948 (Hymenoptera: Encyrtidae), are reported for the first time in Brazil. Specimens of the PHM were collected on nine hosts plants, Annona muricata L. (Anonnaceae), Glycine max (L.) Merr. (Fabaceae), Centrolobium paraensis Tul. (Fabaceae), Inga edulis Mart. (Fabaceae), Hibiscus rosa-sinensis L. (Malvaceae), Psidium guajava L. (Myrtaceae), Averrhoa carambola L. (Oxalidaceae), Citrus sinensis (L.) Osbeck (Rutaceae) and Solanum lycopersicum L. (Solanaceae), in four municipalities in the north-northeast of the state of Roraima. The plants C. paraensis, I. edulis and C. sinensis are recorded for the first time as a hosts for PHM. Characteristic injuries observed on the host plants infested by PHM and suggestions for its management are presented.

  4. Phenology of the Pine Bark Adelgid, Pineus strobi (Hemiptera: Adelgidae), in White Pine Forests of Southwestern Virginia.

    Science.gov (United States)

    Wantuch, Holly A; Kuhar, Thomas P; Salom, Scott M

    2017-12-08

    The pine bark adelgid, Pineus strobi Hartig (Hemiptera: Adelgidae), is a native herbivore of eastern white pine, Pinus strobus L. (Pinales: Pinaceae), in eastern North America. P. strobi does not appear to have any dominant overwintering lifestage in southwest Virginia, as it does in its northern range. Eggs can be found consistently from late March through early December and may be produced sporadically later throughout the winter during warm periods. Two distinct generations were observed in the spring, after which life stage frequencies overlapped. Adult body size varied seasonally and was greatest in the spring. The present study constitutes the first recording of phenological details of the P. strobi in its southern range, informing biological control efforts aimed at closely related invasive pests. The phenological plasticity observed between northern and southern P. strobi populations provides insight into the potential effects of climate on the population dymanics of this and related species. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  5. Metabolic Profiling of <em>Lactococcus lactisem> Under Different Culture Conditions

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    Normah Mohd Noor

    2012-07-01

    Full Text Available Gas chromatography mass spectrometry (GC-MS and headspace gas chromatography mass spectrometry (HS/GC-MS were used to study metabolites produced by <em>Lactococcus lactisem> subsp. <em>cremoris> MG1363 grown at a temperature of 30 °C with and without agitation at 150 rpm, and at 37 °C without agitation. It was observed that <em>L. lactisem> produced more organic acids under agitation. Primary alcohols, aldehydes, ketones and polyols were identified as the corresponding trimethylsilyl (TMS derivatives, whereas amino acids and organic acids, including fatty acids, were detected through methyl chloroformate derivatization. HS analysis indicated that branched-chain methyl aldehydes, including 2-methylbutanal, 3-methylbutanal, and 2-methylpropanal are degdradation products of isoleucine, leucine or valine. Multivariate analysis (MVA using partial least squares discriminant analysis (PLS-DA revealed the major differences between treatments were due to changes of amino acids and fermentation products.

  6. Characterization and functional assay of a fatty acyl-CoA reductase gene in the scale insect, Ericerus pela Chavannes (Hemiptera: Coccoidae).

    Science.gov (United States)

    Hu, Yan-Hong; Chen, Xiao-Ming; Yang, Pu; Ding, Wei-Feng

    2018-04-01

    Ericerus pela Chavannes (Hemiptera: Coccoidae) is an economically important scale insect because the second instar males secrete a harvestable wax-like substance. In this study, we report the molecular cloning of a fatty acyl-CoA reductase gene (EpFAR) of E. pela. We predicted a 520-aa protein with the FAR family features from the deduced amino acid sequence. The EpFAR mRNA was expressed in five tested tissues, testis, alimentary canal, fat body, Malpighian tubules, and mostly in cuticle. The EpFAR protein was localized by immunofluorescence only in the wax glands and testis. EpFAR expression in High Five insect cells documented the recombinant EpFAR reduced 26-0:(S) CoA and to its corresponding alcohol. The data illuminate the molecular mechanism for fatty alcohol biosynthesis in a beneficial insect, E. pela. © 2017 Wiley Periodicals, Inc.

  7. Matérias estranhas em canela em pó e páprica em pó, comercializadas no estado de São Paulo

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    CORREIA Marlene

    2000-01-01

    Full Text Available Com objetivos de padronizar metodologias para matérias estranhas em canela em pó e páprica em pó e de avaliar as condições higiênicas desses condimentos, foram adquiridas, no período de agosto a novembro de 1998, 78 amostras de canela em pó e 56 de páprica em pó, em estabelecimentos comerciais de seis cidades do Estado de São Paulo. Foram utilizados métodos da Association of Official Analytical Chemists International (AOAC - 1995, 16.14.12/968.38b para canela em pó e 16.14.22/977.25B para páprica em pó. Os métodos mostraram-se adequados, sem resíduos interferentes no papel de filtro que dificultasse o diagnóstico da análise e viáveis de serem utilizados nos laboratórios de Microscopia de Alimentos. Os resultados obtidos indicaram 100% das amostras contendo fragmentos de insetos; pêlos de roedor foram isolados em 73,1% de amostras de canela em pó e 34,0% de páprica em pó, enquanto ácaros mortos apareceram, respectivamente, em 37,2% e 12,5% de amostras. É necessário revisão da legislação de alimentos em vigor, com o estabelecimento de níveis de tolerância para fragmentos de insetos.

  8. A new species of the genus Eurhadina Haupt (Hemiptera: Auchenorrhyncha: Cicadellidae: Typhlocybinae) from Korea, with a key to Korean species.

    Science.gov (United States)

    Oh, Sumin; Lim, Jongok; Jung, Sunghoon

    2016-04-11

    The leafhopper genus Eurhadina Haupt, 1929 belongs to the tribe Typhlocybini of subfamily Typhlocybinae (Hemiptera: Auchenorrhyncha: Cicadellidae). Currently, genus Eurhadina includes 3 subgenera, Eurhadina Haupt 1929, Singhardina Mahmood 1967, Zhihadina Yang & Li 1991. A total of 20 valid species of subgenus Eurhadina have been described in the Nearctic and Palaearctic region and the subgenus Singhardina includes 57 species in the Oriental and Palaearctic region (Huang & Zhang 1999, Dworakowska 2002). The subgenus Zhihadina includes only 1 species from China (Yang & Lee, 1991). So far, four species of subgenus Eurhadina were recorded in the Korean Peninsula (Kwon & Huh 2001): Eurhadina (Eurhadina) betularia Anufriev, 1969, E. (E.) koreana Dworakowska, 1971, E. (E.) pulchella (Fallen, 1806), and E. (E.) wagneri Dworakowska, 1969. The majority of species belonging to the subgenus Eurhadina are difficult to distinguish by external appearance because the color patterns of the forewings are very similar among species.

  9. Storage of Euschistus heros Eggs (Fabricius) (Hemiptera: Pentatomidae) in Liquid Nitrogen for Parasitization by Telenomus podisi Ashmead (Hymenoptera: Platygastridae).

    Science.gov (United States)

    Favetti, B M; Butnariu, A R; Doetzer, A K

    2014-06-01

    Records in the literature with regard to the influence of freezing of pentatomid eggs on parasitism by microhymenopterans are scarce. In this research, we compared the storage of Euschistus heros (Fabricius) (Hemiptera: Pentatomidae) eggs in liquid nitrogen for different periods with the objective of optimizing the multiplication of Telenomus podisi Ashmead (Hymenoptera: Platygastridae) in the laboratory. Fresh eggs of E. heros were exposed (S3, S6) or not (NS3, NS6) to UV light for 30 min and stored in 1.5-mL plastic vials in liquid nitrogen either for 3 (S3, NS3) or 6 months (S6, NS6), and egg suitability to parasitoid development was compared to control eggs exposed (SC) or not (NSC) to UV treatment. Global data analysis showed that E. heros eggs stored in liquid nitrogen with or without UV treatment, for 3 or 6 months, were suitable for T. podisi parasitization.

  10. Morphology of the European species of the aphid genus Eulachnus (Hemiptera: Aphididae: Lachninae) - A SEM comparative and integrative study.

    Science.gov (United States)

    Kanturski, Mariusz; Karcz, Jagna; Wieczorek, Karina

    2015-09-01

    Scanning electron microscopy (SEM) methods were used for the first time to elucidate the external morphology of the European species of the genus Eulachnus (Hemiptera: Aphididae: Lachninae), a representative genus of the conifer-feeding aphids tribe Eulachnini. We examined and compared the external morphology of apterous and alate viviparous females from the parthenogenetic generation as well as oviparous females and alate males belonging to the sexual generation. FE-SEM images based on HMDS and cryo-SEM preparation techniques revealed better image quality than the CPD technique in regard to surface tension and morphological signs of cell deteriorations (i.e., existence of depressions, drying artifacts and membrane blebs). Three morphologically different species groups "agilis", "brevipilosus" and "cembrae" were proposed due to the differences in head, antennae, legs and dorsal chaetotaxy as well as dorsal sclerotization. The most characteristic features and differences of representatives of these groups are presented and discussed. Copyright © 2015 Elsevier Ltd. All rights reserved.

  11. Differences in bacterial diversity of host-associated populations of Phylloxera notabilis Pergande (Hemiptera: Phylloxeridae) in pecan and water hickory.

    Science.gov (United States)

    Medina, R F; Nachappa, P; Tamborindeguy, C

    2011-04-01

    Host-associated differentiation (HAD) is the presence of genetically divergent, host-associated populations. It has been suggested that microbial symbionts of insect herbivores may play a role in HAD by allowing their insect hosts to use different plant species. The objective of this study was to document if host-associated populations of Phylloxera notabilis Pergande (Hemiptera: Phylloxeridae) in pecan and water hickory corresponded with differences in the composition of their associated bacteria. To test this hypothesis, we characterized the symbionts present in P. notabilis associated with these two tree species through metagenomic analyses using 454 sequencing. Differences in bacterial diversity were found between P. notabilis populations associated with pecan and water hickory. The bacteria, Pantoea agglomerans and Serratia marcescens, were absent in the P. notabilis water hickory population, whereas both species accounted for more than 69.72% of bacterial abundance in the pecan population. © 2011 The Authors. Journal of Evolutionary Biology © 2011 European Society For Evolutionary Biology.

  12. Identification of <em>Colletotrichum> species causing anthracnose on Tahiti lime, tree tomato and mango

    Directory of Open Access Journals (Sweden)

    Martínez Erika P.

    2009-08-01

    Full Text Available

    In Colombia, citrus, tree tomato and mango crops are likely to suffer considerable losses from anthracnose caused by several <em>Colletotrichum> species, which were identified by the present study on infected organs of the three fruit crops, sampled in different regions of the country. Identification was based on their morphological and molecular characteristics, as well as on fungicide (benomyl and copper hydroxide sensitivity and pathogenicity tests. The latter assessed infectivity on both the original hosting crop and the other two crops (crossed infection, by putting the fungi in contact with organs taken from the three fruit crops. Molecular identification of the <em>Colletotrichum> species was carried out through amplification of rDNA ITS regions by means of <em>C. gloeosporioidesem> (CgInt and <em>C. acutatumem> (CaInt2 specific primer PCR combining the use of ITS4 universal primer. The results indicate that <em>C. acutatumem> is the infectious agent in Tahiti lime and tree tomato, and so is <em>C. gloeosporioidesem> in mango. Although <em>C. acutatumem> is the infectious agent in two diferent fruit species, the strains proved to be specific of their original hosts.

  13. Isolamento de micobactérias em Felis concolor em cativeiro

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    María Julia Traversa

    2009-02-01

    Full Text Available Este trabalho foi realizado em uma reserva natural da Argentina com antecedentes de tuberculose em uma suçuarana adulta. O objetivo foi identificar por meio de técnicas bacteriológicas e de biologia molecular as espécies isoladas da orofaringe de cinco suçuaranas que apresentavam sinais clínicos inespecíficos. As amostras foram colhidas das suçuaranas após sedação. Posteriormente foram processadas para obtenção do isolamento e identificação por meio de provas bioquímicas do gênero Mycobacterium pela técnica de PCR. Investigou-se a presença das seqüências de inserção IS6110 e IS1081 e hsp65. Obtiveram-se resultados positivos à coloração de Ziehl-Neelsen de quatro amostras, isolando cinco cepas de crescimento lento. As cepas foram classificadas como M. gordonae em dois casos e M. simiae, M scrofulaceum e M. avium/intracellulare em um. Por PRA, identificou-se o padrão de M. gordonae em três cepas e M. avium III ou M. simiae em dois.

  14. Marketing em moda

    OpenAIRE

    Leães, Sabrina Durgante

    2008-01-01

    Dissertação de mestrado em Design e Marketing O actual estado do Marketing em Moda é uma das questões ainda complexa com que se debate a sociedade global. As questões do Marketing em Moda percorrem alguns aspectos fundamentais tais como as constantes mutações do meio envolvente, a forma de como é percebida e comunicada a identidade das marcas de moda, em busca da melhor forma de segmentar o mercado e definir o seu posicionamento, bem como a reacção ao produto de moda do consumidor final. ...

  15. Synthesis and Antibacterial Evaluation of New <em>N>-acylhydrazone Derivatives from Dehydroabietic Acid

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    Wen Gu

    2012-04-01

    Full Text Available A series of new <em>N>-acylhydrazone derivatives were synthesized in good yields through the reactions of dehydroabietic acid hydrazide with a variety of substituted arylaldehydes. The structures of the synthesized compounds were confirmed by IR, 1H- and 13C-NMR, ESI-MS, elemental analysis and single crystal X-ray diffraction. From the crystal structure of compound 4l, the C=N double bonds of these <em>N>-acylhydrazones showed (<em>E>-configuration, while the NMR data of compounds 4aq indicated the existence of two rotamers for each compound in solution. The target compounds were evaluated for their antibacterial activities against four microbial strains. The result suggested that several compounds exhibited pronounced antibacterial activities. Particularly, compound 4p exhibited good antibacterial activity against <em>Staphylococcus aureusem> and <em>Bacillus subtilisem> comparable to positive control. The possible antibacterial metabolism and the strategy for further optimization of this compound were also discussed.

  16. A summary of eight traits of Coleoptera, Hemiptera, Orthoptera and Araneae, occurring in grasslands in Germany

    Science.gov (United States)

    Gossner, Martin M.; Simons, Nadja K.; Achtziger, Roland; Blick, Theo; Dorow, Wolfgang H. O.; Dziock, Frank; Köhler, Frank; Rabitsch, Wolfgang; Weisser, Wolfgang W.

    2015-03-01

    Analyses of species traits have increased our understanding of how environmental drivers such as disturbances affect the composition of arthropod communities and related processes. There are, however, few studies on which traits in the arthropod community are affected by environmental changes and which traits affect ecosystem functioning. The assembly of arthropod traits of several taxa is difficult because of the large number of species, limited availability of trait databases and differences in available traits. We sampled arthropod species data from a total of 150 managed grassland plots in three regions of Germany. These plots represent the spectrum from extensively used pastures to mown pastures to intensively managed and fertilized meadows. In this paper, we summarize information on body size, dispersal ability, feeding guild and specialization (within herbivores), feeding mode, feeding tissue (within herbivorous suckers), plant part (within herbivorous chewers), endophagous lifestyle (within herbivores), and vertical stratum use for 1,230 species of Coleoptera, Hemiptera (Heteroptera, Auchenorrhyncha), Orthoptera (Saltatoria: Ensifera, Caelifera), and Araneae, sampled by sweep-netting between 2008 and 2012. We compiled traits from various literature sources and complemented data from reliable internet sources and the authors’ experience.

  17. Acizzia solanicola (Hemiptera: Psyllidae) probing behaviour on two Solanum spp. and implications for possible pathogen spread

    Science.gov (United States)

    Valenzuela, Isabel; Trebicki, Piotr; Powell, Kevin S.; Vereijssen, Jessica; Norng, Sorn

    2017-01-01

    Piercing-sucking insects are vectors of plant pathogens, and an understanding of their feeding behaviour is crucial for studies on insect population dynamics and pathogen spread. This study examines probing behaviour of the eggplant psyllid, Acizzia solanicola (Hemiptera: Psyllidae), using the electrical penetration graph (EPG) technique, on two widespread and common hosts: eggplant (Solanum melongena) and tobacco bush (S. mauritianum). Six EPG waveforms were observed: waveform NP (non-probing phase), waveform C (pathway phase), G (feeding activities in xylem tissues), D (first contact with phloem tissues), E1 (salivation in the sieve elements) and E2 (ingestion from phloem tissues). Results showed that A. solanicola is predominantly a phloem feeder and time spent in salivation and ingestion phases (E1 and E2) differed between hosts. Feeding was enhanced on eggplant compared to tobacco bush which showed some degree of resistance, as evidenced by shorter periods of phloem ingestion, a higher propensity to return to the pathway phase once in the sieve elements and higher number of salivation events on tobacco bush. We discuss how prolonged phloem feeding could indicate the potential for A. solanicola to become an important pest of eggplant and potential pathogen vector. PMID:28575085

  18. Remote sensing and spatial statistical techniques for modelling Ommatissus lybicus (Hemiptera: Tropiduchidae) habitat and population densities.

    Science.gov (United States)

    Al-Kindi, Khalifa M; Kwan, Paul; R Andrew, Nigel; Welch, Mitchell

    2017-01-01

    In order to understand the distribution and prevalence of Ommatissus lybicus (Hemiptera: Tropiduchidae) as well as analyse their current biographical patterns and predict their future spread, comprehensive and detailed information on the environmental, climatic, and agricultural practices are essential. The spatial analytical techniques such as Remote Sensing and Spatial Statistics Tools, can help detect and model spatial links and correlations between the presence, absence and density of O. lybicus in response to climatic, environmental, and human factors. The main objective of this paper is to review remote sensing and relevant analytical techniques that can be applied in mapping and modelling the habitat and population density of O. lybicus . An exhaustive search of related literature revealed that there are very limited studies linking location-based infestation levels of pests like the O. lybicus with climatic, environmental, and human practice related variables. This review also highlights the accumulated knowledge and addresses the gaps in this area of research. Furthermore, it makes recommendations for future studies, and gives suggestions on monitoring and surveillance methods in designing both local and regional level integrated pest management strategies of palm tree and other affected cultivated crops.

  19. Remote sensing and spatial statistical techniques for modelling Ommatissus lybicus (Hemiptera: Tropiduchidae habitat and population densities

    Directory of Open Access Journals (Sweden)

    Khalifa M. Al-Kindi

    2017-08-01

    Full Text Available In order to understand the distribution and prevalence of Ommatissus lybicus (Hemiptera: Tropiduchidae as well as analyse their current biographical patterns and predict their future spread, comprehensive and detailed information on the environmental, climatic, and agricultural practices are essential. The spatial analytical techniques such as Remote Sensing and Spatial Statistics Tools, can help detect and model spatial links and correlations between the presence, absence and density of O. lybicus in response to climatic, environmental, and human factors. The main objective of this paper is to review remote sensing and relevant analytical techniques that can be applied in mapping and modelling the habitat and population density of O. lybicus. An exhaustive search of related literature revealed that there are very limited studies linking location-based infestation levels of pests like the O. lybicus with climatic, environmental, and human practice related variables. This review also highlights the accumulated knowledge and addresses the gaps in this area of research. Furthermore, it makes recommendations for future studies, and gives suggestions on monitoring and surveillance methods in designing both local and regional level integrated pest management strategies of palm tree and other affected cultivated crops.

  20. Ocorrência de Selenaspidus articulatus (Morgan (Hemiptera, Diaspididae e do predador Pentilia egena (Mulsant (Coleoptera, Coccinelidae em Myrtus communis L. (Myrtaceae, em Pinheiral, RJ Occurrence of Selenaspidus articulatus (Morgan (Hemiptera, Diaspididae and of the predator Pentilia egena (Mulsant (Coleoptera, Coccinelidae on Myrtus communis L. (Myrtaceae, in Pinheiral County, Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Edson Henrique de Azeredo

    2004-12-01

    Full Text Available As avaliações foram realizadas, semanalmente, durante os estádios vegetativo e de floração/frutificação em quatro quadrantes (N, S, L, O da planta. Foram coletadas 2.230 espécimes de S. articulatus, sendo 1.224 no estágio vegetativo e 1.006 no florescimento/frutificação, sendo 59,8% adultos. De um total de 965 P. egena, 416 foram imaturos e 549 adultos. As principais conclusões deste trabalho são: (1 M. communis pode ser indicada como planta isca; (2 esta planta pode ser cultivada ou não próxima às culturas de importância econômica, pois funciona como um repositório da praga ou de seu inimigo natural; (3 a relação predador/presa/quadrante da planta/estágio fenológico foi, respectivamente, 1,14:1,0 e 1,0:1,08 nos quadrantes norte e leste; enquanto, à sul e oeste da planta M. communis foi de 1,0:2,26 e 1,0:2,80; (4 a predação de S. articulatus por P. egena ocorreu, em média, de 1,0 a 2,8 ninfas e 1,66 a 4,44 adultos (no estágio vegetativo e de 1,0-3,76 a 1,0-3,98 (no estágio de florescimento/frutificação, com diferenças significativas em PThe evaluations were conducted weekly in the vegetative and blooming/fructification phases in four quadrants (N, S, E, W of the plant. It was collected 2,230 specimens of S. articulatus, 1,224 in the vegetative phase and 1,006 in the blooming/fructification, being 59,8% adults. From the total of 965 of P. egena, 416 were immatures and 549 adults. The main conclusions are: (1 M. communis may be indicated as plant bait; (2 this plant, may be cultivated or not around the crops of economic importance, because it may function as a reservoir of the pest or of its natural enemies; (3 the relation predator/prey/plant quadrant/phenology phases was, respectivelly, 1.14:1.0 and 1.0:1,08 in north and east quadrant; while in south and west quadrant was 1.0:2.26 and 1.0:2.80; and, (4 the predaction of S. articulatus by P. egena was, in average, 1.02.8 nymphs and 1.664.44 adults (in vegetative