Numerical Modeling of Wave Overtopping of Buffalo Harbor Confined Disposal Facility (CDF4)

Science.gov (United States)

2017-10-01

navigation channel , and harbor complex. Though there are anecdotal claims and debris indicating possible sediment movement, there has been no evidence of...littoral sediments inside the disposal area, into the channel and harbor, and other adjacent areas to the CDF4. 1.4 Study plan 1.4.1 Purpose There...locations. 2.4 Maintenance dredging data The need for maintenance dredging arises from sedimentation in the navigation channels , which impedes

Integrated approach to assess ecosystem health in harbor areas.

Science.gov (United States)

Bebianno, M J; Pereira, C G; Rey, F; Cravo, A; Duarte, D; D'Errico, G; Regoli, F

2015-05-01

Harbors are critical environments with strategic economic importance but with potential environmental impact: health assessment criteria are a key issue. An ecosystem health status approach was carried out in Portimão harbor as a case-study. Priority and specific chemical levels in sediments along with their bioavailability in mussels, bioassays and a wide array of biomarkers were integrated in a biomarker index (IBR index) and the overall data in a weight of evidence (WOE) model. Metals, PAHs, PCBs and HCB were not particularly high compared with sediment guidelines and standards for dredging. Bioavailability was evident for Cd, Cu and Zn. Biomarkers proved more sensitive namely changes of antioxidant responses, metallothioneins and vittellogenin-like proteins. IBR index indicated that site 4 was the most impacted area. Assessment of the health status by WOE approach highlighted the importance of integrating sediment chemistry, bioaccumulation, biomarkers and bioassays and revealed that despite some disturbance in the harbor area, there was also an impact of urban effluents from upstream. Environmental quality assessment in harbors. Copyright © 2015 Elsevier B.V. All rights reserved.

76 FR 8653 - Drawbridge Operation Regulation; Gulf Intracoastal Waterway, New Orleans Harbor, Inner Harbor...

Science.gov (United States)

2011-02-15

... Operation Regulation; Gulf Intracoastal Waterway, New Orleans Harbor, Inner Harbor Navigation Canal, New Orleans, Orleans Parish, LA AGENCY: Coast Guard, DHS. ACTION: Notice of temporary deviation from... Lock), at New Orleans, Orleans Parish, Louisiana. This deviation is necessary to replace all of the...

75 FR 78601 - Drawbridge Operation Regulation; Gulf Intracoastal Waterway, New Orleans Harbor, Inner Harbor...

Science.gov (United States)

2010-12-16

... Operation Regulation; Gulf Intracoastal Waterway, New Orleans Harbor, Inner Harbor Navigation Canal, New Orleans, Orleans Parish, LA AGENCY: Coast Guard, DHS. ACTION: Notice of temporary deviation from... Harvey Lock), at New Orleans, Orleans Parish, Louisiana. This deviation is necessary to adjust the...

Charleston Harbor, SC, Regional Sediment Management Study: Beneficial Use of Dredged Material through Nearshore Placement

Science.gov (United States)

2017-05-01

Modeling System (CMS) that were developed and applied previously for the Charleston Harbor numerical modeling study (Kashlan 2013) were used in...Particle Tracking Model; Report 1: Model theory, implementation, and example applications. ERDC/CHL TR-6-20. Vicksburg, MS: U.S. Army Engineer Research ...ER D C/ CH L TR -1 7- 7 Regional Sediment Management (RSM) Program Charleston Harbor, SC, Regional Sediment Management Study

Charleston Harbor, SC, Regional Sediment Management Study; Beneficial Use of Dredged Material through Nearshore Placement

Science.gov (United States)

2017-05-01

Modeling System (CMS) that were developed and applied previously for the Charleston Harbor numerical modeling study (Kashlan 2013) were used in...Particle Tracking Model; Report 1: Model theory, implementation, and example applications. ERDC/CHL TR-6-20. Vicksburg, MS: U.S. Army Engineer Research ...ER D C/ CH L TR -1 7- 7 Regional Sediment Management (RSM) Program Charleston Harbor, SC, Regional Sediment Management Study

75 FR 18056 - Safety Zone; Fireworks Display, Patuxent River, Solomons Island Harbor, MD

Science.gov (United States)

2010-04-09

... the event, and enhancing public and maritime safety. Basis and Purpose Fireworks displays are... promote public and maritime safety during a fireworks display, and to protect mariners transiting the area...-AA00 Safety Zone; Fireworks Display, Patuxent River, Solomons Island Harbor, MD AGENCY: Coast Guard...

33 CFR 207.610 - St. Lawrence River, Cape Vincent Harbor, N.Y.; use, administration, and navigation of the harbor...

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false St. Lawrence River, Cape Vincent Harbor, N.Y.; use, administration, and navigation of the harbor and U.S. breakwater. 207.610 Section 207... NAVIGATION REGULATIONS § 207.610 St. Lawrence River, Cape Vincent Harbor, N.Y.; use, administration, and...

Geoscience rediscovers Phoenicia's buried harbors

Science.gov (United States)

Marriner, Nick; Morhange, Christophe; Doumet-Serhal, Claude; Carbonel, Pierre

2006-01-01

After centuries of archaeological debate, the harbors of Phoenicia's two most important city states, Tyre and Sidon, have been rediscovered, and including new geoarcheological results reveal how, where, and when they evolved after their Bronze Age foundations. The early ports lie beneath their present urban centers, and we have indentified four harbor phases. (1) During the Bronze Age, Tyre and Sidon were characterized by semi-open marine coves that served as protoharbors. (2) Biostratigraphic and lithostratigraphic data indicate the presence of early artificial basins after the first millennium B.C. (3) The harbors reached their apogees during the Greco-Roman and Byzantine periods. (4) Silting up and coastal progradation led to burial of the medieval basins, lost until now.

33 CFR 165.904 - Lake Michigan at Chicago Harbor & Burnham Park Harbor-Safety and Security Zone.

Science.gov (United States)

2010-07-01

... & Burnham Park Harbor-Safety and Security Zone. 165.904 Section 165.904 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION... Guard District § 165.904 Lake Michigan at Chicago Harbor & Burnham Park Harbor—Safety and Security Zone...

Case study of small harbor excitation under storm and tsunami conditions

Science.gov (United States)

Synolakis, Costas; Maravelakis, Nikos; Kalligeris, Nikos; Skanavis, Vassilios; Kanoglu, Utku; Yalciner, Ahmet; Lynett, Pat

2016-04-01

Simultaneous nearshore and interior-to-ports wave and current measurements for small ports are not common, and few, if any, benchmarking cases at sufficient resolution exist to help validate numerical model of intermediate waves, or even long waves. The wave conditions inside the old Venetian harbor of Chania, Greece and offshore were measured and studied from 2012 to 2015. The construction of this harbor began in the 14th century, and since then, its layout has been modified to adapt to different social and to economic conditions. It is divided into a western and an eastern basin. The eastern basin is used by recreational vessels and fishing boats throughout the year. The western basin has an exposed entrance to the north, and it is essentially functional half of the year, because of the severe overtopping and flooding that occur during the northern winter storms. Our work is motivated by the necessity to protect the monument from severe winter storm conditions and allow safe mooring and all other recreational activities that take place in the exposed western basin. Two earlier studies had proposed the construction of a low crested breakwater near the harbor entrance. The first design has been partially constructed, while the second never materialized. The main disadvantage of both studies was the lack of any wave field measurements. At the same time, second order or complimentary phenomena such as harbor resonance had not been considered. To address the lack of field data, the offshore wave climate has been monitored since October 2012 using an AWAC 600kHz instrument, deployed at 23m depth. The response of the western and eastern basins of the harbor was measured with a TWR-2050 (deployed at 5.5m depth) and an RBRDuet T.D./wave (deployed at 2m depth) pressure gauges respectively. Significant wave heights ranging up to 5.8 m with significant periods of up to 10 sec were measured. The harbor pressure gauges are now being re-deployed in other locations to collect

A Probabilistic and Observation Based Methodology to Estimate Small Craft Harbor Vulnerability to Tsunami Events

Science.gov (United States)

Keen, A. S.; Lynett, P. J.; Ayca, A.

2016-12-01

Because of the damage resulting from the 2010 Chile and 2011 Japanese tele-tsunamis, the tsunami risk to the small craft marinas in California has become an important concern. The talk will outline an assessment tool which can be used to assess the tsunami hazard to small craft harbors. The methodology is based on the demand and structural capacity of the floating dock system, composed of floating docks/fingers and moored vessels. The structural demand is determined using a Monte Carlo methodology. Monte Carlo methodology is a probabilistic computational tool where the governing might be well known, but the independent variables of the input (demand) as well as the resisting structural components (capacity) may not be completely known. The Monte Carlo approach uses a distribution of each variable, and then uses that random variable within the described parameters, to generate a single computation. The process then repeats hundreds or thousands of times. The numerical model "Method of Splitting Tsunamis" (MOST) has been used to determine the inputs for the small craft harbors within California. Hydrodynamic model results of current speed, direction and surface elevation were incorporated via the drag equations to provide the bases of the demand term. To determine the capacities, an inspection program was developed to identify common features of structural components. A total of six harbors have been inspected ranging from Crescent City in Northern California to Oceanside Harbor in Southern California. Results from the inspection program were used to develop component capacity tables which incorporated the basic specifications of each component (e.g. bolt size and configuration) and a reduction factor (which accounts for the component reduction in capacity with age) to estimate in situ capacities. Like the demand term, these capacities are added probabilistically into the model. To date the model has been applied to Santa Cruz Harbor as well as Noyo River. Once

77 FR 59551 - Safety Zone, Changes to Original Rule; Boston Harbor's Rock Removal Project, Boston Inner Harbor...

Science.gov (United States)

2012-09-28

... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 [Docket No. USCG-2012-0767] RIN 1625-AA00 Safety Zone, Changes to Original Rule; Boston Harbor's Rock Removal Project, Boston Inner Harbor... original provisions of that temporary final rule, but adds two additional safety zones necessary for the...

High Resolution Tsunami Modeling and Assessment of Harbor Resilience; Case Study in Istanbul

Science.gov (United States)

Cevdet Yalciner, Ahmet; Aytore, Betul; Gokhan Guler, Hasan; Kanoglu, Utku; Duzgun, Sebnem; Zaytsev, Andrey; Arikawa, Taro; Tomita, Takashi; Ozer Sozdinler, Ceren; Necmioglu, Ocal; Meral Ozel, Nurcan

2014-05-01

Ports and harbors are the major vulnerable coastal structures under tsunami attack. Resilient harbors against tsunami impacts are essential for proper, efficient and successful rescue operations and reduction of the loss of life and property by tsunami disasters. There are several critical coastal structures as such in the Marmara Sea. Haydarpasa and Yenikapi ports are located in the Marmara Sea coast of Istanbul. These two ports are selected as the sites of numerical experiments to test their resilience under tsunami impact. Cargo, container and ro-ro handlings, and short/long distance passenger transfers are the common services in both ports. Haydarpasa port has two breakwaters with the length of three kilometers in total. Yenikapi port has one kilometer long breakwater. The accurate resilience analysis needs high resolution tsunami modeling and careful assessment of the site. Therefore, building data with accurate coordinates of their foot prints and elevations are obtained. The high resolution bathymetry and topography database with less than 5m grid size is developed for modeling. The metadata of the several types of structures and infrastructure of the ports and environs are processed. Different resistances for the structures/buildings/infrastructures are controlled by assigning different friction coefficients in a friction matrix. Two different tsunami conditions - high expected and moderate expected - are selected for numerical modeling. The hybrid tsunami simulation and visualization codes NAMI DANCE, STOC-CADMAS System are utilized to solve all necessary tsunami parameters and obtain the spatial and temporal distributions of flow depth, current velocity, inundation distance and maximum water level in the study domain. Finally, the computed critical values of tsunami parameters are evaluated and structural performance of the port components are discussed in regard to a better resilience. ACKNOWLEDGEMENTS: Support by EU 603839 ASTARTE Project, UDAP-Ç-12

[Pearl Harbor.

Science.gov (United States)

Johnson, Jennifer, Ed.

1992-01-01

This issue of "Loblolly Magazine" was written in observance of the 50th anniversary of the U.S. entrance into World War II. The publication features interviews conducted by East Texas high school students with Clarence Otterman, one of the few survivors of the crew of the USS Arizona, which was bombed during the attack on Pearl Harbor,…

Vibration-based structural health monitoring of harbor caisson structure

Science.gov (United States)

Lee, So-Young; Lee, So-Ra; Kim, Jeong-Tae

2011-04-01

This study presents vibration-based structural health monitoring method in foundation-structure interface of harbor caisson structure. In order to achieve the objective, the following approaches are implemented. Firstly, vibration-based response analysis method is selected and structural health monitoring (SHM) technique is designed for harbor caisson structure. Secondly, the performance of designed SHM technique for harbor structure is examined by FE analysis. Finally, the applicability of designed SHM technique for harbor structure is evaluated by dynamic tests on a lab-scaled caisson structure.

32 CFR 765.6 - Regulations for Pearl Harbor, Hawaii.

Science.gov (United States)

2010-07-01

... 32 National Defense 5 2010-07-01 2010-07-01 false Regulations for Pearl Harbor, Hawaii. 765.6... RULES RULES APPLICABLE TO THE PUBLIC § 765.6 Regulations for Pearl Harbor, Hawaii. The Commander, U.S. Naval Base, Pearl Harbor, Hawaii, is responsible for prescribing and enforcing such rules and...

33 CFR 110.238 - Apra Harbor, Guam.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Apra Harbor, Guam. 110.238 Section 110.238 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.238 Apra Harbor, Guam. (a) The anchorage grounds (Datum: WGS...

The Suez Canal and the petroleum harbors

International Nuclear Information System (INIS)

Anon.

1997-01-01

The Suez Canal is the second longest channel in the world and allows to save 60% of the travel time between the petroleum harbors of the Arabic peninsula and Europe. This short paper gives a summary of the main petroleum harbors activity along the channel from the Red sea to the Mediterranean sea. (J.S.)

Polycyclic aromatic hydrocarbons and trace elements bounded to airborne PM10 in the harbor of Volos, Greece: Implications for the impact of harbor activities

Science.gov (United States)

Manoli, E.; Chelioti-Chatzidimitriou, A.; Karageorgou, K.; Kouras, A.; Voutsa, D.; Samara, C.; Kampanos, I.

2017-10-01

Harbors are often characterized by high levels of air pollutants that are emitted from ship traffic and other harbor activities. In the present study, the concentrations of Polycyclic Aromatic Hydrocarbons (PAHs) and trace elements (As, Cd, Ni, Pb, Cr, Mn, Zn, and Fe) bounded to the inhalable particulate matter PM10 were studied in the harbor of Volos, central Greece, during a 2-year period (2014-2015). Seasonal and daily variations were investigated. Moreover, total carcinogenic and mutagenic activities of PAHs were calculated. The effect of major wind sectors (sea, city, industrial, harbor) was estimated to assess the potential contribution of ship traffic and harbor activities, such as scrap metal handling operations. Results showed that the harbor sector (calm winds ≤ 0.5 m s-1) was associated with the highest concentrations of PM10. The harbor sector was also associated with relatively increased levels of trace elements (As, Fe, Cr, Mn, Ni), however the effect of this sector was lower than the corresponding effect of the industrial wind sector. The sea sector showed only a slight increase in B[a]Py and Σ12PAHs, whereas the highest increasing effect for PAHs and traffic-related elements, such as Pb and Zn, was evidenced for the city sector.

Summary of oceanographic and water–quality measurements in West Falmouth Harbor and Buzzards Bay, Massachusetts, 2009–2010

Science.gov (United States)

Ganju, Neil K.; Dickhudt, Patrick J.; Thomas, Jennifer A.; Borden, Jonathan; Sherwood, Christopher R.; Montgomery, Ellyn T.; Twomey, Erin R.; Martini, Marinna A.

2011-01-01

This data report presents oceanographic and water-quality observations made at six locations in West Falmouth Harbor and Buzzards Bay, Massachusetts, from August 2009 to September 2010. Both Buzzards Bay and West Falmouth Harbor are estuarine embayments; the input of freshwater on the eastern margin of Buzzards Bay adjacent to Cape Cod and West Falmouth Harbor is largely due to groundwater. In West Falmouth Harbor, the groundwater that seeps into the harbor is characterized by relatively high levels of nitrate. This high nitrate load has modified the ecology of the harbor (Howes and others, 2006) and may be a significant source of nitrate to Buzzards Bay during seasons with low biological nitrate uptake. The U.S. Geological Survey undertook these measurements to improve understanding of circulation, residence time, and water quality in the harbor and bay. We set up and monitored multiple sites in both Buzzards Bay and West Falmouth Harbor, measuring depth, water velocity,salinity, pH, dissolved oxygen, chlorophyll-a, and nitrate concentration. In this report we present the processed time-series data at these locations and provide access to the data and metadata. The results will be used to understand circulation mechanisms and verify numerical models of hydrodynamics and biogeochemistry.

The potential of epigenetics in stress-enhanced fear learning models of PTSD.

Science.gov (United States)

Blouin, Ashley M; Sillivan, Stephanie E; Joseph, Nadine F; Miller, Courtney A

2016-10-01

Prolonged distress and dysregulated memory processes are the core features of post-traumatic stress disorder (PTSD) and represent the debilitating, persistent nature of the illness. However, the neurobiological mechanisms underlying the expression of these symptoms are challenging to study in human patients. Stress-enhanced fear learning (SEFL) paradigms, which encompass both stress and memory components in rodents, are emerging as valuable preclinical models of PTSD. Rodent models designed to study the long-term mechanisms of either stress or fear memory alone have identified a critical role for numerous epigenetic modifications to DNA and histone proteins. However, the epigenetic modifications underlying SEFL remain largely unknown. This review will provide a brief overview of the epigenetic modifications implicated in stress and fear memory independently, followed by a description of existing SEFL models and the few epigenetic mechanisms found to date to underlie SEFL. The results of the animal studies discussed here highlight neuroepigenetics as an essential area for future research in the context of PTSD through SEFL studies, because of its potential to identify novel candidates for neurotherapeutics targeting stress-induced pathogenic memories. © 2016 Blouin et al.; Published by Cold Spring Harbor Laboratory Press.

Wave Climate and Wave Response, 2025 Plan, Kahului Harbor, Maui, Hawaii

National Research Council Canada - National Science Library

Thompson, Edward

2002-01-01

... (wind waves and swell) and long waves (harbor oscillations), was used to evaluate the technical feasibility of three alternative modifications to the harbor, including the Kahului Commercial Harbor 2025 Master Plan...

33 CFR 162.165 - Buffalo and Rochester Harbors, New York.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Buffalo and Rochester Harbors, New York. 162.165 Section 162.165 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... and Rochester Harbors, New York. In Buffalo and Rochester Harbors, no vessel may exceed 6 miles per...

Satellite Monitoring of Boston Harbor Water Quality: Initial Investigations

Science.gov (United States)

Sheldon, P.; Chen, R. F.; Schaaf, C.; Pahlevan, N.; Lee, Z.

2016-02-01

The transformation of Boston Harbor from the "dirtiest in America" to a National Park Area is one of the most remarkable estuarine recoveries in the world. A long-term water quality dataset from 1991 to present exists in Boston Harbor due to a $3. 8 billion lawsuit requiring the harbor clean-up. This project uses discrete water sampling and underway transects with a towed vehicle coordinated with Landsat 7 and Landsat 8 to create surface maps of chlorophyll a (Chl a), dissolved organic matter (CDOM and DOC), total suspended solids (TSS), diffuse attenuation coefficient (Kd_490), and photic depth in Boston Harbor. In addition, 3 buoys have been designed, constructed, and deployed in Boston Harbor that measure Chl a and CDOM fluorescence, optical backscatter, salinity, temperature, and meteorological parameters. We are initially using summer and fall of 2015 to develop atmospheric corrections for conditions in Boston Harbor and develop algorithms for Landsat 8 data to estimate in water photic depth, TSS, Chl a, Kd_490, and CDOM. We will report on initial buoy and cruise data and show 2015 Landsat-derived distributions of water quality parameters. It is our hope that once algorithms for present Landsat imagery can be developed, historical maps of water quality can be constructed using in water data back to 1991.

Old Harbor Scammon Bay Hydro Feasibility

Energy Technology Data Exchange (ETDEWEB)

Brent Petrie

2007-06-27

The grantee, Alaska Village Electric Cooperative (AVEC), is a non-profit member owned rural electric generation and distribution cooperative. The proposed Project is located near the community of Old Harbor, Alaska. Old Harbor is on the southeastern coast of Kodiak Island, approximately 70 miles southwest of the City of Kodiak and 320 miles southwest of Anchorage. In 1998 sufficient information had been developed to apply for a license to construct the project and the cost was estimated to be $2,445,000 for a 500 KW project on Lagoon Creek. Major features of the project included an eight-foot high diversion dam on Mountain Creek, a desander box, a 9,800-foot long penstock to the powerhouse on Lagoon Creek, and a 5,500-foot long access road. It was also anticipated that the project could provide an additional source of water to Old Harbor. The report details the history and lessons learned in designing and permiting the proposed hydroelectric facility.

33 CFR 80.1450 - Nawiliwili Harbor, Kauai, HI.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Nawiliwili Harbor, Kauai, HI. 80.1450 Section 80.1450 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Pacific Islands § 80.1450 Nawiliwili Harbor, Kauai, HI...

33 CFR 80.1470 - Kawaihae Harbor, Hawaii, HI.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Kawaihae Harbor, Hawaii, HI. 80.1470 Section 80.1470 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Pacific Islands § 80.1470 Kawaihae Harbor, Hawaii, HI...

33 CFR 110.208 - Buffalo Harbor, N.Y.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Buffalo Harbor, N.Y. 110.208 Section 110.208 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.208 Buffalo Harbor, N.Y. (a) The anchorage grounds—(1...

Effects of Harbor Modification on Crescent City, California's Tsunami Vulnerability

Science.gov (United States)

Dengler, Lori; Uslu, Burak

2011-06-01

More damaging tsunamis have impacted Crescent City, California in historic times than any other location on the West Coast of the USA. Crescent City's harbor has undergone significant modification since the early 20th century, including construction of several breakwaters, dredging, and a 200 × 300 m2 small boat basin. In 2006, a M w 8.3 earthquake in the Kuril Islands generated a moderate Pacific-wide tsunami. Crescent City recorded the highest amplitudes of any tide gauge in the Pacific and was the only location to experience structural damage. Strong currents damaged docks and boats within the small boat basin, causing more than US 20 million in damage and replacement costs. We examine how modifications to Crescent City's harbor may have affected its vulnerability to moderate tsunamis such as the 2006 event. A bathymetric grid of the basin was constructed based on US Army Corps of Engineers soundings in 1964 and 1965 before the construction of the small boat basin. The method of splitting tsunamis was used to estimate tsunami water heights and current velocities at several locations in the harbor using both the 1964-1965 grid and the 2006 bathymetric grid for the 2006 Kuril event and a similar-sized source along the Sanriku coast of Japan. Model velocity outputs are compared for the two different bathymetries at the tide gauge location and at six additional computational sites in the harbor. The largest difference between the two grids is at the small boat basin entrance, where the 2006 bathymetry produces currents over three times the strength of the currents produced by the 1965 bathymetry. Peak currents from a Sanriku event are comparable to those produced by the 2006 event, and within the boat basin may have been higher. The modifications of the harbor, and in particular the addition of the small boat basin, appear to have contributed to the high current velocities and resulting damage in 2006 and help to explain why the 1933 M w 8.4-8.7 Sanriku tsunami

Tech Talk for Social Studies Teachers Lest We Forget: Remembering Pearl Harbor.

Science.gov (United States)

Green, Tim

2001-01-01

Presents an annotated bibliography that provides Web sites about Pearl Harbor (Hawaii). Includes Web sites that cover Pearl Harbor history, a live view of Pearl Harbor, stories from people who remember where they were during the attack, information on the naval station at Pearl Harbor, and a virtual tour of the USS Arizona. (CMK)

33 CFR 80.1460 - Kahului Harbor, Maui, HI.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Kahului Harbor, Maui, HI. 80.1460 Section 80.1460 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Pacific Islands § 80.1460 Kahului Harbor, Maui, HI. A line drawn...

33 CFR 80.1480 - Hilo Harbor, Hawaii, HI.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Hilo Harbor, Hawaii, HI. 80.1480 Section 80.1480 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Pacific Islands § 80.1480 Hilo Harbor, Hawaii, HI. A line drawn...

Madaket Harbor, Nantucket, Massachusetts. Water Resources Improvement.

Science.gov (United States)

1977-07-01

will continue to be, important increases in the recreational use of land and water. The harbor area is an important arena for commercial shellfishing...an important arena for commercial shell fishing. The past few years have seen a rather rapid increase in residential land use. Construction has...beamc. Tnis material will be re-deposited,, viaj troio it 1-apfro1inr ox prior location. j, MADAKET HARBOR NANTUCKET, MASSACHUSETTS FEASIBILITY

Hydraulic experiment on flow and topography change in harbor due to tsunami and its numerical simulation

International Nuclear Information System (INIS)

Fujii, Naoki; Ikeno, Masaaki; Sakakiyama, Tsutomu; Matsuyama, Masafumi; Takao, Makoto; Mukohara, Takeshi

2009-01-01

Numerical model of topography change is important to examine collapse of the harbor facilities by sand transport due to tsunami. Problems for evaluation of sand transport due to tsunami with topography change model are in precision of the numerical model and topography change data. Therefore, we installed the harbor in large-scaled wave tank and carried out experiment about tsunami flow and topography change to get those detailed data. For results provided by experimental test, we applied the topography change model of Ikeno et al. (2009a) and evaluated it about the reproduction characteristics. As a result, it was confirmed that reproduction of an experiment improved by using new pickup rate formula proposed by Ikeno et al. (2009a). (author)

77 FR 45239 - Amendment of Class E Airspace; Bar Harbor, ME

Science.gov (United States)

2012-07-31

...-1366; Airspace Docket No. 11-ANE-13] Amendment of Class E Airspace; Bar Harbor, ME AGENCY: Federal... area at Bar Harbor, ME, as the Surry Non-Directional Radio Beacon (NDB) has been decommissioned and new Standard Instrument Approach Procedures have been developed at Hancock County-Bar Harbor Airport. This...

Underwater noise from three types of offshore wind turbines: estimation of impact zones for harbor porpoises and harbor seals.

Science.gov (United States)

Tougaard, Jakob; Henriksen, Oluf Damsgaard; Miller, Lee A

2009-06-01

Underwater noise was recorded from three different types of wind turbines in Denmark and Sweden (Middelgrunden, Vindeby, and Bockstigen-Valar) during normal operation. Wind turbine noise was only measurable above ambient noise at frequencies below 500 Hz. Total sound pressure level was in the range 109-127 dB re 1 microPa rms, measured at distances between 14 and 20 m from the foundations. The 1/3-octave noise levels were compared with audiograms of harbor seals and harbor porpoises. Maximum 1/3-octave levels were in the range 106-126 dB re 1 microPa rms. Maximum range of audibility was estimated under two extreme assumptions on transmission loss (3 and 9 dB per doubling of distance, respectively). Audibility was low for harbor porpoises extending 20-70 m from the foundation, whereas audibility for harbor seals ranged from less than 100 m to several kilometers. Behavioral reactions of porpoises to the noise appear unlikely except if they are very close to the foundations. However, behavioral reactions from seals cannot be excluded up to distances of a few hundred meters. It is unlikely that the noise reaches dangerous levels at any distance from the turbines and the noise is considered incapable of masking acoustic communication by seals and porpoises.

New Harbor in Kangerlussuaq, Western Greenland

DEFF Research Database (Denmark)

Stenstad, Jaran Gjerlandj; Eppeland, Kjetil Grødal; Ingeman-Nielsen, Thomas

2015-01-01

transported by rivers from the inland ice to the inner parts of the fjord. These sediment layers reduce the water depth and prevent container- and cruiseships to dock, imposing large additional maintenance costs, and inefficient operability. Through engineering geological field and lab investigations......, a possible new harbor location around 10 km further out the fjord near Hancock Pynt, has been investigated. The onshore area was found to be highly suitable for a harbor support area, where a sub-base thickness of 1.8 m with gravel cover-layer was found adequate for the calculated design loads. Existing...

33 CFR 165.1402 - Apra Outer Harbor, Guam-regulated navigation area.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Apra Outer Harbor, Guam-regulated....1402 Apra Outer Harbor, Guam—regulated navigation area. (a) The following is a regulated navigation area—The waters of the Pacific Ocean and Apra Outer Harbor enclosed by a line beginning at latitude 13...

Ground-water status report, Pearl Harbor area, Hawaii, 1978

Science.gov (United States)

Soroos, Ronald L.; Ewart, Charles J.

1979-01-01

Increasing demand for freshwater in Hawaii has placed heavy stress on many of the State 's basal aquifer systems. The most heavily stressed of these systems is the Pearl Harbor on Oahu. The Pearl Harbor basal aquifer supplies as much as 277 million gallons per day. Since early in this century, spring discharge has been declining while pumpage has been increasing. Total ground-water discharge has remained steady despite short-term fluctuations. Some wells show general increases in chloride concentration while others remain steady. Chloride concentrations throughout the area show no apparent increase since 1970. Basal water head maps of the Pearl Harbor area clearly reflect the natural discharge points, which are the springs located along the shore near the center of Pearl Harbor. Basal-water hydrographs show a general decline of about 0.09 foot per year. This implies depletion of storage at a rate of about 25 million gallons per day. (USGS).

Alaska Harbor Seal Glacial Surveys

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Floating glacial ice serves as a haul-out substrate for a significant number (10-15%) of Alaskan harbor seals, and thus surveying tidewater glacial fjords is an...

32 CFR 705.31 - USS Arizona Memorial, Pearl Harbor.

Science.gov (United States)

2010-07-01

... 32 National Defense 5 2010-07-01 2010-07-01 false USS Arizona Memorial, Pearl Harbor. 705.31... NAVY REGULATIONS AND OFFICIAL RECORDS PUBLIC AFFAIRS REGULATIONS § 705.31 USS Arizona Memorial, Pearl Harbor. (a) Limited space and the desirability of keeping the Memorial simple and dignified require the...

76 FR 32071 - Safety Zone; Conneaut Festival Fireworks, Conneaut Harbor, Conneaut, OH

Science.gov (United States)

2011-06-03

...-AA00 Safety Zone; Conneaut Festival Fireworks, Conneaut Harbor, Conneaut, OH AGENCY: Coast Guard, DHS... Conneaut Harbor, Conneaut, OH for the Conneaut Festival Fireworks. This zone is intended to restrict vessels from a portion of Conneaut Harbor, Conneaut, OH during the Conneaut Festival Fireworks on July 3...

Remembering Pearl Harbor at 75 Years.

Science.gov (United States)

Liehr, Patricia; Sopcheck, Janet; Milbrath, Gwyneth

2016-12-01

: On December 7, 1941, the Sunday-morning quiet of the U.S. naval base in Pearl Harbor, Hawaii, was shattered by dive-bombing Japanese fighter planes. The planes came in two waves-and when it was all over, more than 2,400 were killed and more than 1,100 were injured.Nurses were stationed at U.S. Naval Hospital Pearl Harbor, Tripler General Hospital (now Tripler Army Medical Center), Hickam Field Hospital, Schofield Barracks Station Hospital, and aboard the USS Solace, and witnessed the devastation. But they also did what nurses do in emergencies-they responded and provided care to those in need. Here are the stories of a few of those nurses.

CRSMP Potential Harbor Borrow Sites 2012

Data.gov (United States)

California Natural Resource Agency — Harbor locations as identified originally in the California Shoreline Database compiled by Noble Consultants (Jon Moore) for California Department of Boating and...

Hazardous substances shipping at inland water harbors

International Nuclear Information System (INIS)

Benkovic, Z.

2009-01-01

Safety measures and regulations system covering the aspects of fire protection, professional and ecological safety are aimed to create a safe working environment, by detection and remedy of conditions that are potentially hazardous for the well-being of the employees or are leading to certain undesired events. Such unwanted incidents may result in different consequences: operating person's injury, environment pollution or material damage. This study attempts to illustrate the organization of work during hazardous matter loading and unloading at inland water harbors, based on legal provisions and decrees involving safety precautions, and in order to achieve constant enhancement of operating procedure, decreasing thereby the number of work-related injuries and various accidental situations. Fundamental precondition required to prevent possible accidents and to optimize general safety policy is to recognize and control any danger or potential hazard, as well as to be familiar with the legal provisions covering the inland waterway transport of harmful substances.(author)

Efficacy of Daptomycin Monotherapy and In Combination with β-lactams for Daptomycin-Susceptible Enterococcus faecium Harboring LiaSR Substitutions: Influence of The Inoculum Effect.

Science.gov (United States)

Kebriaei, Razieh; Rice, Seth A; Singh, Kavindra V; Stamper, Kyle C; Dinh, An Q; Rios, Rafael; Diaz, Lorena; Murray, Barbara E; Munita, Jose M; Tran, Truc T; Arias, Cesar A; Rybak, Michael J

2018-05-14

Enterococcus faecium that harbor LiaFSR substitutions but are phenotypically susceptible to daptomycin (DAP) by current breakpoints are problematic since predisposition to resistance may lead to therapeutic failure. Using a simulated endocardial vegetation (SEV) PK/PD model, we investigated DAP regimens (6, 8 and 10 mg/kg/day) as monotherapy and in combination with ampicillin (AMP), ceftaroline (CPT) or ertapenem (ERT) against E. faecium HOU503, a DAP-susceptible strain that harbors common LiaS and LiaR substitutions found in clinical isolates (T120S and W73C, respectively). Of interest, the efficacy of DAP monotherapy, at any dose regimen, was dependent on the size of the inoculum. At an inoculum of ∼10 9 CFU/g, DAP doses of 6-8 mg/kg/d were not effective and led to significant regrowth with emergence of resistant derivatives. In contrast, at an inoculum of ∼10 7 , marked reductions in bacterial counts were observed with DAP 6 mg/kg/d with no resistance. The inoculum effect was confirmed in a rat model using humanized DAP exposures. Combinations of DAP with AMP, CPT or ERT demonstrated enhanced eradication and reduced potential for resistance allowing for de-escalation of the DAP dose. Persistence of the LiaRS substitutions were identified in DAP-resistant isolates recovered from the SEV model and in DAP-resistant derivatives of an initially DAP-susceptible clinical isolate of E. faecium (HOU668) harboring LiaSR substitutions and recovered from a patient with a recurrent bloodstream infection. Our results provide novel data for the use of DAP monotherapy and combinations for recalcitrant E. faecium infections and paves the way for testing these approaches in humans. Copyright © 2018 American Society for Microbiology.

33 CFR 334.1430 - Apra Inner Harbor, Island of Guam; restricted area.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Apra Inner Harbor, Island of Guam... THE ARMY, DEPARTMENT OF DEFENSE DANGER ZONE AND RESTRICTED AREA REGULATIONS § 334.1430 Apra Inner Harbor, Island of Guam; restricted area. (a) The restricted area. The waters within Apra Inner Harbor and...

Harbor seal vibrissa morphology suppresses vortex-induced vibrations

OpenAIRE

Hanke, Wolf; Witte, Mathias; Miersch, Lars; Brede, Martin; Oeffner, Johannes; Michael, Mark; Hanke, Frederike; Leder, Alfred; Dehnhardt, Guido

2010-01-01

Harbor seals (Phoca vitulina) often live in dark and turbid waters, where their mystacial vibrissae, or whiskers, play an important role in orientation. Besides detecting and discriminating objects by direct touch, harbor seals use their whiskers to analyze water movements, for example those generated by prey fish or by conspecifics. Even the weak water movements left behind by objects that have passed by earlier can be sensed and followed accurately (hydrodynamic trail following). While scan...

Production of infectious chimeric hepatitis C virus genotype 2b harboring minimal regions of JFH-1.

Science.gov (United States)

Murayama, Asako; Kato, Takanobu; Akazawa, Daisuke; Sugiyama, Nao; Date, Tomoko; Masaki, Takahiro; Nakamoto, Shingo; Tanaka, Yasuhito; Mizokami, Masashi; Yokosuka, Osamu; Nomoto, Akio; Wakita, Takaji

2012-02-01

To establish a cell culture system for chimeric hepatitis C virus (HCV) genotype 2b, we prepared a chimeric construct harboring the 5' untranslated region (UTR) to the E2 region of the MA strain (genotype 2b) and the region of p7 to the 3' UTR of the JFH-1 strain (genotype 2a). This chimeric RNA (MA/JFH-1.1) replicated and produced infectious virus in Huh7.5.1 cells. Replacement of the 5' UTR of this chimera with that from JFH-1 (MA/JFH-1.2) enhanced virus production, but infectivity remained low. In a long-term follow-up study, we identified a cell culture-adaptive mutation in the core region (R167G) and found that it enhanced virus assembly. We previously reported that the NS3 helicase (N3H) and the region of NS5B to 3' X (N5BX) of JFH-1 enabled replication of the J6CF strain (genotype 2a), which could not replicate in cells. To reduce JFH-1 content in MA/JFH-1.2, we produced a chimeric viral genome for MA harboring the N3H and N5BX regions of JFH-1, combined with a JFH-1 5' UTR replacement and the R167G mutation (MA/N3H+N5BX-JFH1/R167G). This chimeric RNA replicated efficiently, but virus production was low. After the introduction of four additional cell culture-adaptive mutations, MA/N3H+N5BX-JFH1/5am produced infectious virus efficiently. Using this chimeric virus harboring minimal regions of JFH-1, we analyzed interferon sensitivity and found that this chimeric virus was more sensitive to interferon than JFH-1 and another chimeric virus containing more regions from JFH-1 (MA/JFH-1.2/R167G). In conclusion, we established an HCV genotype 2b cell culture system using a chimeric genome harboring minimal regions of JFH-1. This cell culture system may be useful for characterizing genotype 2b viruses and developing antiviral strategies.

33 CFR 110.87 - Henderson Harbor, N.Y.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Henderson Harbor, N.Y. 110.87... ANCHORAGE REGULATIONS Special Anchorage Areas § 110.87 Henderson Harbor, N.Y. (a) Area A. The area in the... latitude 43°51′08.8″ N, longitude 76°12′08.9″ W, thence to latitude 43°51′09.0″ N, longitude 76°12′19.0″ W...

Influenza A(H10N7) Virus in Dead Harbor Seals, Denmark

DEFF Research Database (Denmark)

Krog, Jesper Schak; Hansen, Mette Sif; Holm, Elisabeth

2015-01-01

Since April 2014, an outbreak of influenza in harbor seals has been ongoing in northern Europe. In Denmark during June-August, 152 harbor seals on the island of Anholt were found dead from severe pneumonia. We detected influenza A(H10N7) virus in 2 of 4 seals examined.......Since April 2014, an outbreak of influenza in harbor seals has been ongoing in northern Europe. In Denmark during June-August, 152 harbor seals on the island of Anholt were found dead from severe pneumonia. We detected influenza A(H10N7) virus in 2 of 4 seals examined....

Harbor seal vibrissa morphology suppresses vortex-induced vibrations.

Science.gov (United States)

Hanke, Wolf; Witte, Matthias; Miersch, Lars; Brede, Martin; Oeffner, Johannes; Michael, Mark; Hanke, Frederike; Leder, Alfred; Dehnhardt, Guido

2010-08-01

Harbor seals (Phoca vitulina) often live in dark and turbid waters, where their mystacial vibrissae, or whiskers, play an important role in orientation. Besides detecting and discriminating objects by direct touch, harbor seals use their whiskers to analyze water movements, for example those generated by prey fish or by conspecifics. Even the weak water movements left behind by objects that have passed by earlier can be sensed and followed accurately (hydrodynamic trail following). While scanning the water for these hydrodynamic signals at a swimming speed in the order of meters per second, the seal keeps its long and flexible whiskers in an abducted position, largely perpendicular to the swimming direction. Remarkably, the whiskers of harbor seals possess a specialized undulated surface structure, the function of which was, up to now, unknown. Here, we show that this structure effectively changes the vortex street behind the whiskers and reduces the vibrations that would otherwise be induced by the shedding of vortices from the whiskers (vortex-induced vibrations). Using force measurements, flow measurements and numerical simulations, we find that the dynamic forces on harbor seal whiskers are, by at least an order of magnitude, lower than those on sea lion (Zalophus californianus) whiskers, which do not share the undulated structure. The results are discussed in the light of pinniped sensory biology and potential biomimetic applications.

Aerial Survey Counts of Harbor Seals in Coastal Alaska (1998-2002)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This dataset supports efforts to estimate the abundance and trends in population size of Alaska harbor seals. Annual surveys of harbor seal populations are...

Aerial Survey Counts of Harbor Seals in Coastal Alaska (2003-2011)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This dataset supports efforts to estimate the abundance and trends in population size of Alaska harbor seals. Annual surveys of harbor seal populations are...

78 FR 18479 - Drawbridge Operation Regulations; Inner Harbor Navigation Canal, New Orleans, LA

Science.gov (United States)

2013-03-27

... Operation Regulations; Inner Harbor Navigation Canal, New Orleans, LA AGENCY: Coast Guard, DHS. ACTION... across the Inner Harbor Navigation Canal, mile 4.6, at New Orleans, Louisiana. This deviation is... Seabrook Highway crossing the Inner Harbor Navigation Canal, mile 4.6, in New Orleans, Louisiana. The...

Faunal and vegetation monitoring in response to harbor dredging in the Port of Miami

Science.gov (United States)

Daniels, Andre; Stevenson, Rachael; Smith, Erin; Robblee, Michael

2018-04-11

Seagrasses are highly productive ecosystems. A before-after-control-impact (BACI) design was used to examine effects of dredging on seagrasses and the animals that inhabit them. The control site North Biscayne Bay and the affected site Port of Miami had seagrass densities decrease during both the before, Fish and Invertebrate Assessment Network 2006-2011, and after, Faunal Monitoring in Response to Harbor Dredging 2014-2016, studies. Turbidity levels increased at North Biscayne Bay and Port of Miami basins during the Faunal Monitoring in Response to Harbor Dredging study, especially in 2016. Animal populations decreased significantly in North Biscayne Bay and Port of Miami in the Faunal Monitoring in Response to Harbor Dredging study compared to the Fish and Invertebrate Assessment Network study. Predictive modeling shows that numbers of animal populations will likely continue to decrease if the negative trends in seagrass densities continue unabated. There could be effects on several fisheries vital to the south Florida economy. Additional research could determine if animal populations and seagrass densities have rebounded or continued to decrease.

76 FR 50489 - Agency Information Collection Activities: Harbor Maintenance Fee

Science.gov (United States)

2011-08-15

... Activities: Harbor Maintenance Fee AGENCY: U.S. Customs and Border Protection, Department of Homeland... Fee (CBP Forms 349 and 350). This is a proposed extension of an information collection that was... Fee. OMB Number: 1651-0055. Form Number: CBP Forms 349 and 350. Abstract: The Harbor Maintenance Fee...

Pearl Harbor: lessons for the dam safety community

Energy Technology Data Exchange (ETDEWEB)

Martin, T.E. [AMEC Earth and Environmental Ltd., Burnaby, BC (Canada)

2001-10-01

Every good dam safety program must be based on surveillance and emergency response planning. The same principles apply to the gathering of information for military intelligence and the planning of defence tactics. Lessons learned from failure have spurred the advancement of dam engineering. Dam safety experts can benefit from the inadequacies encountered by the military community, with the most famous occurring on December 7, 1941 in Pearl Harbor. Both intelligence gathering and contingency response planning failed miserably. The data was not properly disseminated, interpreted, analysed. The proper response to the situation was not initiated. Human error and failure to communicate are the two main reasons that explain the debacle. The inquiries into the tragedy at Pearl Harbor provided valuable lessons, related to individual and organizational failures, which the authors shared in this presentation. The relevance to dam safety was made. All Federal Bureau of Investigation (FBI) agents must read the lessons drawn from Pearl Harbor, as they have responsibility for dam safety. 4 refs.

33 CFR 110.27 - Lynn Harbor in Broad Sound, Mass.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Lynn Harbor in Broad Sound, Mass. 110.27 Section 110.27 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.27 Lynn Harbor in Broad Sound, Mass. North of...

33 CFR 117.458 - Inner Harbor Navigation Canal, New Orleans.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Inner Harbor Navigation Canal, New Orleans. 117.458 Section 117.458 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Harbor Navigation Canal, New Orleans. (a) The draws of the SR 46 (St. Claude Avenue) bridge, mile 0.5...

Extrachromosomal deoxyribonucleic acid in R factor-harboring Enterobacteriaceae

DEFF Research Database (Denmark)

Møller, JK; Bak, AL; Christiansen, C

1976-01-01

Extrachromosomal deoxyribonucleic acid (DNA) from 24 different R factor-harboring Enterobacteriaceae was isolated and characterized by analytical ultracentrifugation and electron microscopy. The R factors represented 15 different patterns of transferable drug resistance found in enterobacteria from...... from 1.700 to 1.720 g/cm3. The majority of the bacteria contained extrachromosomal DNAs of various densities. Three-fourths of the R factors were classified as fi+. The investigation illustrates the extensive variability in the physical characteristics of plasmid DNA from R factor-harboring strains....

Genetics Home Reference: Floating-Harbor syndrome

Science.gov (United States)

... Patton MA, Hurst J, Donnai D, McKeown CM, Cole T, Goodship J. Floating-Harbor syndrome. J Med ... medicine? What is newborn screening? New Pages Lyme disease Fibromyalgia White-Sutton syndrome All New & Updated Pages ...

76 FR 38153 - California State Nonroad Engine Pollution Control Standards; Commercial Harbor Craft Regulations...

Science.gov (United States)

2011-06-29

... Standards; Commercial Harbor Craft Regulations; Opportunity for Public Hearing and Comment AGENCY... engines on commercial harbor craft. CARB has requested that EPA issue a new authorization under [email protected] . SUPPLEMENTARY INFORMATION: I. California's Commercial Harbor Craft Regulations In a...

33 CFR 117.753 - Ship Channel, Great Egg Harbor Bay.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Ship Channel, Great Egg Harbor Bay. 117.753 Section 117.753 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements New Jersey § 117.753 Ship Channel, Great Egg Harbor Bay. The draw of the S52 (Ship...

Congress Investigates: Pearl Harbor and 9/11 Congressional Hearing Exhibits

Science.gov (United States)

Blackerby, Christine

2011-01-01

On the morning of December 7, 1941, Japanese bombers staged a surprise attack on U.S. military forces at Pearl Harbor in Hawaii. Sixty years after the attack on Pearl Harbor, the United States was attacked again. On the morning of September 11, 2001, four commercial airplanes hijacked by 19 terrorists killed nearly 3,000 people when they crashed…

Genetic characterization of blaNDM-harboring plasmids in carbapenem-resistant Escherichia coli from Myanmar.

Directory of Open Access Journals (Sweden)

Yo Sugawara

Full Text Available The bacterial enzyme New Delhi metallo-β-lactamase hydrolyzes almost all β-lactam antibiotics, including carbapenems, which are drugs of last resort for severe bacterial infections. The spread of carbapenem-resistant Enterobacteriaceae that carry the New Delhi metallo-β-lactamase gene, blaNDM, poses a serious threat to public health. In this study, we genetically characterized eight carbapenem-resistant Escherichia coli isolates from a tertiary care hospital in Yangon, Myanmar. The eight isolates belonged to five multilocus-sequence types and harbored multiple antimicrobial-resistance genes, resulting in resistance against nearly all of the antimicrobial agents tested, except colistin and fosfomycin. Nine plasmids harboring blaNDM genes were identified from these isolates. Multiple blaNDM genes were found in the distinct Inc-replicon types of the following plasmids: an IncA/C2 plasmid harboring blaNDM-1 (n = 1, IncX3 plasmids harboring blaNDM-4 (n = 2 or blaNDM-7 (n = 1, IncFII plasmids harboring blaNDM-4 (n = 1 or blaNDM-5 (n = 3, and a multireplicon F plasmid harboring blaNDM-5 (n = 1. Comparative analysis highlighted the diversity of the blaNDM-harboring plasmids and their distinct characteristics, which depended on plasmid replicon types. The results indicate circulation of phylogenetically distinct strains of carbapenem-resistant E. coli with various plasmids harboring blaNDM genes in the hospital.

Current and historic mercury deposition to New Haven Harbor (CT, USA): Implications for industrial coastal environments

International Nuclear Information System (INIS)

Clark, Heather F.; Benoit, Gaboury

2009-01-01

This study quantifies historic and current mercury contamination in New Haven Harbor (New Haven, Connecticut, USA) through the analysis of sediment cores. The mercury concentration measured in surface sediment ranged from 320 to 1640 μg kg -1 with an average of 530 μg kg -1 . The harbor is relatively small in area (6.6 km 2 ) but displays a large range in concentrations, illustrating the important methodological issue that a large number of samples may be necessary to capture the variability in even a small area. Depth profiles of mercury reflect sedimentation over a range of 20 to 200 years and indicate a complex history of contamination. Mercury depth profiles were compared with lead, copper, cadmium, and silver concentrations and the metals generally covary. This trend indicates that the sources of mercury and heavy metals are linked and that regionally specific sources dominate the historic input of metals rather than large-scale atmospheric deposition patterns. Results also show there are large differences in absolute concentrations of metals among sites in the harbor. Differences in the abundance of Fe-rich, fine-grained sediment likely control the level of metals in various parts of the harbor. Proximity to current sources and the long, diverse industrial history of the harbor also influence the distribution pattern. All of the cores can be modeled as mixing between pre-industrial sediments and either one or two pollution endmembers. This study demonstrates the importance of riverine sources in the mass balance of mercury delivered to coastal areas and of watershed management to preserve coastal ecosystems.

Numerical Simulation of Borehole Flow in Deep Monitor Wells, Pearl Harbor Aquifer, Oahu, Hawaii

Science.gov (United States)

Rotzoll, K.; Oki, D. S.; El-Kadi, A. I.

2010-12-01

Salinity profiles collected from uncased deep monitor wells are commonly used to monitor freshwater-lens thickness in coastal aquifers. However, vertical flow in these wells can cause the measured salinity to differ from salinity in the adjacent aquifer. Substantial borehole flow has been observed in uncased wells in the Pearl Harbor aquifer, Oahu, Hawaii. A numerical modeling approach, incorporating aquifer hydraulic characteristics and recharge rates representative of the Pearl Harbor aquifer, was used to evaluate the effects of borehole flow on measured salinity profiles from deep monitor wells. Borehole flow caused by vertical hydraulic gradients associated with the natural regional groundwater-flow system and local groundwater withdrawals was simulated. Model results were used to estimate differences between vertical salinity profiles in deep monitor wells and the adjacent aquifer in areas of downward, horizontal, and upward flow within the regional flow system—for cases with and without nearby pumped wells. Aquifer heterogeneity, represented in the model as layers of contrasting permeability, was incorporated in model scenarios. Results from this study provide insight into the magnitude of the differences between vertical salinity profiles from deep monitor wells and the salinity distributions in the aquifers. These insights are relevant and are critically needed for management and predictive modeling purposes.

Sedimentation and chronology of heavy metal pollution in Oslo harbor, Norway

International Nuclear Information System (INIS)

Lepland, Aivo; Andersen, Thorbjorn J.; Lepland, Aave; Arp, Hans Peter H.; Alve, Elisabeth; Breedveld, Gijs D.; Rindby, Anders

2010-01-01

Stratigraphic profiles of Cu, Cd and Hg in ten sediment cores from the Oslo harbor, Norway, combined with results of radiometric dating demonstrate that pollution by these metals peaked between 1940 and 1970. Dating results indicate that Hg discharges peaked between 1940 and 1950, Cd reached maximum ca. 1955-1960, and Cu has the highest concentration in sediment interval corresponding to ca. 1970. Geochemical profiles and maxima of Cu, Cd and Hg concentrations can be used as chronostratigraphic markers for sediment cores from the Oslo harbor. Acoustic backscatter and sediment core data indicate that propeller wash affects the seabed in the Oslo harbor. The propeller-induced turbulence causes erosion, and in places exposes and remobilizes contaminated sediments that accumulated in the harbor during previous decades. Such re-exposure of contaminated sediments could be detrimental to local ecosystems and offset remediation efforts, warranting further impact studies and potential mitigation strategies to prevent redistribution.

77 FR 50916 - Safety Zone; Boston Harbor's Rock Removal Project, Boston Inner Harbor, Boston, MA

Science.gov (United States)

2012-08-23

... operation on the navigable waters of Boston Inner Harbor, in the main ship channel near Castle Island. This... operations in support of the U.S. Army Corps of Engineers rock removal project. Entering into, transiting... before the start date of the project. The U.S. Army Corps of Engineers also discussed the rock removal...

33 CFR 80.1490 - Apra Harbor, U.S. Territory of Guam.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Apra Harbor, U.S. Territory of Guam. 80.1490 Section 80.1490 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY INTERNATIONAL NAVIGATION RULES COLREGS DEMARCATION LINES Pacific Islands § 80.1490 Apra Harbor, U...

33 CFR 110.214 - Los Angeles and Long Beach harbors, California.

Science.gov (United States)

2010-07-01

... Angeles Harbor). A circular area with a radius of 400 yards (approximately 366 meters), centered in... 400 Transportation Corridor. (C) Outer Harbor: The western boundary of Commercial Anchorage B. (2... Thence along a line described as an arc, radius of 460 meters (approximately 1509 feet) centered on 33...

33 CFR 110.250 - St. Thomas Harbor, Charlotte Amalie, V.I.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false St. Thomas Harbor, Charlotte... SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.250 St. Thomas Harbor, Charlotte Amalie.... Thomas, V.I. of the United States and approaches thereto, including all waters under its jurisdiction, as...

Littoral cells and harbor dredging along the California coast

Science.gov (United States)

Griggs, Gary B.

1987-02-01

Beach compartments or littoral cells form the framework for our understanding of the sources, transport, and sinks of sand in the nearshore zone. In general, along the California coast, beach sand is derived from rivers or clifferosion, moves alongshore under the influence of the prevailing waver conditions, and ultimately is lost either to a submarine canyon or a dune field. Marinas or harbors built either between or at the upcoast ends of beach compartments have been relatively maintenance-free, because of a lack of significant littoral drift at these locations. On the other hand, those harbors built in the middle reaches or at the downcoast ends of littoral cells have had expensive annual dredging problems, because of the interruption of large volumes of littoral drift. Although engineers have labored for years on various breakwater, jetty, or entrance channel configurations, the actual design utilized is of secondary importance. The critical factors are harbor location within a littoral cell and annual litoral drift volume.

33 CFR 110.129a - Apra Harbor, Guam. (Datum: WGS 84)

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Apra Harbor, Guam. (Datum: WGS 84) 110.129a Section 110.129a Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.129a Apra Harbor, Guam. (Datum: WGS 84) (a...

Pearl Harbor Biological Survey

Science.gov (United States)

1974-08-30

Pearl Harbor also receives Irrigation tailgate waters from the Oahu Sug- ar Company, Industrial waste waters from the Prlmo Brewery , and heated waters...34Observations of the Cell Structure of Salt Fingers", J. Fluid Mech. 41:4, pp 707-719. ~) 3.3-79 ,’:•.-. ^ IV s’V- EFFECTS OF SHIP ACTIVITY Paul L...anticyclonic) death assemblage - in this report, an assemblage (q.v.) of remains (such as shells or bones ) from a naturally occurring association of living

Stabilization and in situ management of radioactive contaminated sediments of Port Hope harbor

International Nuclear Information System (INIS)

Dolinar, G.M.; Killey, R.W.D.; Philipase, K.E.

1991-01-01

This paper presents the findings of laboratory and field studies undertaken to assess the feasibility of in situ management of contaminated sediments in Port Hope harbor. The contaminated sediments stem from historic releases from an adjacent radium and uranium refinery, and uranium, arsenic, and radium are the most abundant contaminants. With improved emission controls, currently accumulating sediments have much lower levels of contamination, and the harbor waters currently meet water quality limits for the contaminants of concern. Within a few years, however, the continuing sedimentation will render the harbor unusable. Field tests have confirmed the dredging will result in incomplete removal of the contaminated sediments and that sediment suspension and the release of pores waters during dredging will produce harbor water contaminant concentrations that would require the treatment of large volumes of water. In addition, no remedial work can start until a site for the dredged material can be found. The local community inquired whether in situ burial of the sediments and abandonment of the harbor would provide safe disposal

Reducing Vulnerability of Ports and Harbors to Earthquake and Tsunami Hazards

Science.gov (United States)

Wood, Nathan J.; Good, James W.; Goodwin, Robert F.

2002-01-01

Recent scientific research suggests the Pacific Northwest could experience catastrophic earthquakes in the near future, both from distant and local sources, posing a significant threat to coastal communities. Damage could result from numerous earthquake-related hazards, such as severe ground shaking, soil liquefaction, landslides, land subsidence/uplift, and tsunami inundation. Because of their geographic location, ports and harbors are especially vulnerable to these hazards. Ports and harbors, however, are important components of many coastal communities, supporting numerous activities critical to the local and regional economy and possibly serving as vital post-event, response-recovery transportation links. A collaborative, multi-year initiative is underway to increase the resiliency of Pacific Northwest ports and harbors to earthquake and tsunami hazards, involving Oregon Sea Grant (OSG), Washington Sea Grant (WSG), the National Oceanic and Atmospheric Administration Coastal Services Center (CSC), and the U.S. Geological Survey Center for Science Policy (CSP). Specific products of this research, planning, and outreach initiative include a regional stakeholder issues and needs assessment, a community-based mitigation planning process, a Geographic Information System (GIS) — based vulnerability assessment methodology, an educational web-site and a regional data archive. This paper summarizes these efforts, including results of two pilot port-harbor community projects, one in Yaquina Bay, Oregon and the other in Sinclair Inlet, Washington. Finally, plans are outlined for outreach to other port and harbor communities in the Pacific Northwest and beyond, using "getting started" workshops and a web-based tutorial.

Pearl Harbor, Hawaii 1 arc-second DEM

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The 1/3-second Pearl Harbor Hawaii Elevation Grid provides bathymetric data in ASCII raster format of 1/3-second resolution in geographic coordinates. This grid is...

76 FR 34865 - Safety Zone; Rochester Harbor Festival, Genesee River, Rochester, NY

Science.gov (United States)

2011-06-15

...-AA00 Safety Zone; Rochester Harbor Festival, Genesee River, Rochester, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the Genesee River, Rochester, NY for the Rochester Harbor Festival fireworks. This zone is intended to...

76 FR 81904 - Solicitation of New Safe Harbors and Special Fraud Alerts

Science.gov (United States)

2011-12-29

... Medicaid Patient and Program Protection Act of 1987, Public Law 100-93 Sec. 14, the Act, Sec. 1128B(b), 42...-called ``safe harbor'' provisions, specifying various payment and business practices that, although... basis for administrative sanctions. OIG safe harbor provisions have been developed ``to limit the reach...

Effect of infrared radiation A on photoaged hairless mice harboring eumelanin and pheomelanin in the epidermis.

Science.gov (United States)

Okazaki, Shizuka; Funasaka, Yoko; Wakamatsu, Kazumasa; Kawana, Seiji; Saeki, Hidehisa

2015-04-01

Infrared radiation A (IRA) is absorbed by melanin and generates heat. Therefore, the effect of IRA could be well analyzed using skin, which contains melanin in the epidermis. Hairless mice harboring epidermal melanocytes that produce eumelanin, pheomelanin, or non-melanin were generated by backcrossing K14-stem cell factor mice, recessive yellow mice, and then albino hairless mice. High-dose IRA was irradiated over 18 weeks after the establishment of photoaged mice by irradiation with ultraviolet B (UVB) three times a week for 14 weeks. Tumor formation was assessed every week. The formation of cyclobutane pyrimidine dimer and apoptotic cells by the irradiation of IRA and UVB was evaluated. Repetitive irradiation of IRA did not promote tumor formation in all types of mice. Pre-irradiation of IRA to UVB, but not post-irradiation, accelerated the elimination of cyclobutane pyrimidine dimers and enhanced apoptosis; these effects were most obvious in eumelanin-producing mice. Real-time polymerase chain reaction analysis showed downregulation of FLICE (cellular caspase 8)-like inhibitory protein and B-cell lymphoma-extra large and upregulation of Bcl-2-associated X protein by UVB, but further enhancement of these molecules by pre-irradiation of IRA was not observed. These results indicate that IRA does not confer the promotion of UVB-induced carcinogenesis in photoaged mice harboring epidermal melanocytes and that photochemical reaction between IRA and melanin might be involved in the induction of apoptosis and the elimination of cyclobutane pyrimidine dimers by UVB. The enhancement of apoptosis by pre-irradiation of IRA to UVB might be induced by mechanisms other than the modification of the mRNA expression of FLICE (cellular caspase 8)-like inhibitory protein, B-cell lymphoma-extra large, and Bcl-2-associated X. © 2015 Japanese Dermatological Association.

Harbor Seal (Phoca vitulina) Reproductive Advertisement Behavior and the Effects of Vessel Noise

Science.gov (United States)

Matthews, Leanna P.

Harbor seals (Phoca vitulina) are a widely distributed pinniped species that mate underwater. Similar to other aquatically mating pinnipeds, male harbor seals produce vocalizations during the breeding season that function in male-male interactions and possibly as an attractant for females. I investigated multiple aspects of these reproductive advertisement displays in a population of harbor seals in Glacier Bay National Park and Preserve, Alaska. First, I looked at vocal production as a function of environmental variables, including season, daylight, and tidal state. Vocalizations were highly seasonal and detection of these vocalizations peaked in June and July, which correspond with the estimated time of breeding. Vocalizations also varied with light, with the lowest probability of detection during the day and the highest probability of detection at night. The high probability of detection corresponded to when females are known to forage. These results are similar to the vocal behavior of previously studied populations. However, unlike previously studied populations, the detection of harbor seal breeding vocalizations did not vary with tidal state. This is likely due to the location of the hydrophone, as it was not near the haul out and depth was therefore not significantly influenced by changes in tidal height. I also investigated the source levels and call parameters of vocalizations, as well as call rate and territoriality. The average source level of harbor seal breeding vocalizations was 144 dB re 1 ?Pa at 1 m and measurements ranged from 129 to 149 dB re 1 ?Pa. Analysis of call parameters indicated that vocalizations of harbor seals in Glacier Bay were similar in duration to other populations, but were much lower in frequency. During the breeding season, there were two discrete calling areas that likely represent two individual males; the average call rate in these display areas was approximately 1 call per minute. The harbor seal breeding season also

33 CFR 110.31 - Hull Bay and Allerton Harbor at Hull, Mass.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Hull Bay and Allerton Harbor at Hull, Mass. 110.31 Section 110.31 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Special Anchorage Areas § 110.31 Hull Bay and Allerton Harbor at...

75 FR 81556 - Solicitation of New Safe Harbors and Special Fraud Alerts

Science.gov (United States)

2010-12-28

... statute (section 1128B(b) of the Social Security Act), as well as developing new OIG Special Fraud Alerts... of New Safe Harbors and Special Fraud Alerts AGENCY: Office of Inspector General (OIG), HHS. ACTION... the public on recommendations for developing new or revised safe harbors and Special Fraud Alerts...

33 CFR 207.580 - Buffalo Harbor, N.Y.; use, administration, and navigation.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Buffalo Harbor, N.Y.; use, administration, and navigation. 207.580 Section 207.580 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.580 Buffalo Harbor, N.Y.; use...

Telemetry data from satellite tags deployed on harbor seals in Cook Inlet, Alaska

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Between 2004 and 2006 we conducted four harbor seal tagging trips in Cook Inlet during the months of October and May. In total, we captured and released 93 harbor...

77 FR 76434 - Solicitation of New Safe Harbors and Special Fraud Alerts

Science.gov (United States)

2012-12-28

... statute (section 1128B(b) of the Social Security Act), as well as developing new OIG Special Fraud Alerts... of New Safe Harbors and Special Fraud Alerts AGENCY: Office of Inspector General (OIG), HHS. ACTION... revised safe harbors and Special Fraud Alerts. Please assist us by referencing the file code OIG-121-N...

78 FR 78807 - Solicitation of New Safe Harbors and Special Fraud Alerts

Science.gov (United States)

2013-12-27

... statute (section 1128B(b) of the Social Security Act), as well as developing new OIG Special Fraud Alerts... of New Safe Harbors and Special Fraud Alerts AGENCY: Office of Inspector General (OIG), HHS. ACTION... revised safe harbors and Special Fraud Alerts. Please assist us by referencing the file code OIG-122-N...

33 CFR 165.754 - Safety Zone: San Juan Harbor, San Juan, PR.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Safety Zone: San Juan Harbor, San Juan, PR. 165.754 Section 165.754 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND... Zone: San Juan Harbor, San Juan, PR. (a) Regulated area. A moving safety zone is established in the...

Respiratory properties of blood in the harbor porpoise, Phocoena phocoena

DEFF Research Database (Denmark)

Soegaard, Lisette B; Hansen, Marie N; van Elk, Cornelis

2012-01-01

Harbor porpoises are active divers that exchange O(2) and CO(2) with the environment during a fast single breath upon surfacing. We investigated blood O(2)-transporting properties, buffer characteristics, Cl(-) transport via the erythrocyte anion exchanger (AE1), circulating nitric oxide metaboli......Harbor porpoises are active divers that exchange O(2) and CO(2) with the environment during a fast single breath upon surfacing. We investigated blood O(2)-transporting properties, buffer characteristics, Cl(-) transport via the erythrocyte anion exchanger (AE1), circulating nitric oxide....... The true plasma non-bicarbonate buffer value was moderately higher than in terrestrial mammals and increased upon deoxygenation. Plasma bicarbonate was also relatively high, contributing to increase the overall buffer capacity. The apparent Cl(-) permeability of harbor porpoise erythrocytes was similar......, plasma nitrate and hemoglobin-mediated nitrite reduction were elevated compared with mammalian standards, suggesting that increased nitric oxide bioavailability and nitrite-derived nitric oxide could play important roles in diving physiology....

Evaluation of Sediment Contamination in Pearl Harbor

Science.gov (United States)

1992-06-01

ancient Hawaiians, was a large natural inland lagoon. Numerous walled fishponds located inside the harbor were used to cultivate various species of fishes... Ecotoxicology , Commission on Natural Resources, National Academy Press, Washington, D.C., 103 pp. National Research Council, 1989. Contaminated Marine

Enhancing Learning in Statistics Classes Through The Use of Concrete Historical Examples: The Space Shuttle Challenger, Pearl Harbor, and the RMS Titanic.

Science.gov (United States)

Schumm, Walter R.; Webb, Farrell J.; Castelo, Carlos S.; Akagi, Cynthia G.; Jensen, Erick J.; Ditto, Rose M.; Spencer Carver, Elaine; Brown, Beverlyn F.

2002-01-01

Discusses the use of historical events as examples for teaching college level statistics courses. Focuses on examples of the space shuttle Challenger, Pearl Harbor (Hawaii), and the RMS Titanic. Finds real life examples can bridge a link to short term experiential learning and provide a means for long term understanding of statistics. (KDR)

Pearl Harbor bombing attack: a contamination legacy revealed in the sedimentary record

International Nuclear Information System (INIS)

Ashwood, T.L.; Olsen, C.R.

1988-01-01

Sediment cores collected from Middle Loch and West Loch of Pearl Harbor were dated using fallout 137 Cs and excess 210 Pb and analysed for several major elements and trace metals. Results indicate that sediment deposited immediately following the 7 December, 1941, bombing attack on Pearl Harbor contained elevated levels of several trace metals. (author)

Animation Augmented Reality Book Model (AAR Book Model) to Enhance Teamwork

Science.gov (United States)

Chujitarom, Wannaporn; Piriyasurawong, Pallop

2017-01-01

This study aims to synthesize an Animation Augmented Reality Book Model (AAR Book Model) to enhance teamwork and to assess the AAR Book Model to enhance teamwork. Samples are five specialists that consist of one animation specialist, two communication and information technology specialists, and two teaching model design specialists, selected by…

Environmental space management in the harbor of Amsterdam, Netherlands; Milieuruimtemanagement haven Amsterdam

Energy Technology Data Exchange (ETDEWEB)

Klok, L.; Hulskotte, J. [TNO Built Environment and Geosciences, Den Haag (Netherlands); Van Breemen, T. [Haven Amsterdam, Amsterdam (Netherlands)

2012-02-15

A new calculation tool will quickly offer the Harbor of Amsterdam insight in the effect of activities in the harbor on the air quality and hence the available environmental space. [Dutch] Een nieuw rekeninstrument geeft Haven Amsterdam snel inzicht in het effect van alle activiteiten in de haven op de luchtkwaliteit en daarmee in de beschikbare milieuruimte.

Pearl Harbor bombing attack: a contamination legacy revealed in the sedimentary record

Energy Technology Data Exchange (ETDEWEB)

Ashwood, T.L.; Olsen, C.R.

1988-02-01

Sediment cores collected from Middle Loch and West Loch of Pearl Harbor were dated using fallout /sup 137/Cs and excess /sup 210/Pb and analysed for several major elements and trace metals. Results indicate that sediment deposited immediately following the 7 December, 1941, bombing attack on Pearl Harbor contained elevated levels of several trace metals.

Army Engineers at Pearl Harbor

Science.gov (United States)

2011-01-01

siblings, he was the grandson of David Belden Lyman—a Christian missionary from New England who settled in the Hilo , Hawaii area—and the descendent of...of Hawaii appeared over Oahu. Some headed for Ameri- can warships at Pearl Harbor and the planes on the ground at nearby Hickam Field; oth- ers...hit Schofield Barracks, Wheeler Field, and Bellows Field. USACE in Hawaii con- sisted of Soldier-engineers in the Army’s Hawaiian Depart- ment and

Multivariate analysis of heavy metal contaminations in seawater and sediments from a heavily industrialized harbor in Southern Taiwan

International Nuclear Information System (INIS)

Lin, Yung-Chang; Chang-Chien, Guo-Ping; Chiang, Pen-Chi; Chen, Wei-Hsiang; Lin, Yuan-Chung

2013-01-01

Highlights: • Kaohsiung Harbor is the largest international commercial port in Taiwan. • The metal distributions in the seawater and sediments were investigated. • Many metals exhibited higher levels of enrichment inside the harbor. • Multivariate statistical analysis was used to characterize the metal pollutions. • Two complex arrays of contamination behaviors exist inside and outside the harbor. -- Abstract: Heavy metal pollution, including chromium, zinc, arsenic, cadmium, mercury, copper, lead, and aluminum, in the largest industrial harbor in southern Taiwan was investigated. Increasing metal contamination was observed by monitoring heavy metal concentrations in seawater and sediments and estimating the enrichment factors, particularly those inside the harbor. Compared to other metal-polluted harbors worldwide, the presence of chromium in the sediments was relatively high. Excluding the background contribution, the harbor area was polluted by outflows from river mouths, wastewater discharging pipes, and point sources near industrial activities within the harbor. It is shown by principal component and cluster analyses that metal contamination was affected by a wide range of different and complex contamination mechanisms inside and outside the harbor, suggesting managing the pollution using straightforward strategies, i.e., solutions that only consider a single source or single pathway of metal emissions, is problematic

The hearing threshold of a harbor porpoise (Phocoena phocoena) for impulsive sounds (L)

NARCIS (Netherlands)

Kastelein, R.A.; Gransier, R.; Hoek, L.; Jong, C.A.F. de

2012-01-01

The distance at which harbor porpoises can hear underwater detonation sounds is unknown, but depends, among other factors, on the hearing threshold of the species for impulsive sounds. Therefore, the underwater hearing threshold of a young harbor porpoise for an impulsive sound, designed to mimic a

Enterobacter cloacae Complex Isolates Harboring blaNMC-A or blaIMI-Type Class A Carbapenemase Genes on Novel Chromosomal Integrative Elements and Plasmids.

Science.gov (United States)

Boyd, David A; Mataseje, Laura F; Davidson, Ross; Delport, Johannes A; Fuller, Jeff; Hoang, Linda; Lefebvre, Brigitte; Levett, Paul N; Roscoe, Diane L; Willey, Barbara M; Mulvey, Michael R

2017-05-01

Carbapenem-resistant Enterobacter cloacae complex isolates submitted to a reference laboratory from 2010 to 2015 were screened by PCR for seven common carbapenemase gene groups, namely, KPC, NDM, OXA-48, VIM, IMP, GES, and NMC-A/IMI. Nineteen of the submitted isolates (1.7%) were found to harbor Ambler class A bla NMC-A or bla IMI -type carbapenemases. All 19 isolates were resistant to at least one carbapenem but susceptible to aminoglycosides, trimethoprim-sulfamethoxazole, tigecycline, and ciprofloxacin. Most isolates (17/19) gave positive results with the Carba-NP test for phenotypic carbapenemase detection. Isolates were genetically diverse by pulsed-field gel electrophoresis macrorestriction analysis, multilocus sequence typing, and hsp60 gene analysis. The genes were found in various Enterobacter cloacae complex species; however, bla NMC-A was highly associated with Enterobacter ludwigii Whole-genome sequencing and bioinformatics analysis revealed that all NMC-A ( n = 10), IMI-1 ( n = 5), and IMI-9 ( n = 2) producers harbored the carbapenemase gene on EludIMEX-1-like integrative mobile elements (EcloIMEXs) located in the identical chromosomal locus. Two novel genes, bla IMI-5 and bla IMI-6 , were harbored on different IncFII-type plasmids. Enterobacter cloacae complex isolates harboring bla NMC-A/IMI -type carbapenemases are relatively rare in Canada. Though mostly found integrated into the chromosome, some variants are located on plasmids that may enhance their mobility potential. © Crown copyright 2017.

75 FR 4693 - Drawbridge Operation Regulation; Inner Harbor Navigational Canal, New Orleans, LA

Science.gov (United States)

2010-01-29

... Operation Regulation; Inner Harbor Navigational Canal, New Orleans, LA AGENCY: Coast Guard, DHS. ACTION... C. Simon) Bascule Bridge across the Inner Harbor Navigational Canal, mile 4.6, at New Orleans, LA... Ochsner Ironman 70.3 New Orleans event. This deviation allows the bridge to remain closed during the event...

Bluff evolution along coastal drumlins: Boston Harbor Islands, Massachusetts

Science.gov (United States)

Himmelstoss, E.A.; FitzGerald, D.M.; Rosen, P.S.; Allen, J.R.

2006-01-01

A series of partially drowned drumlins forms the backbone of the inner islands within Boston Harbor. The shoreline of these rounded glacial deposits is composed of actively retreating bluffs formed by continual wave attack. Comparisons of bluffs reveal variability in their height and lateral extent, as well as in the dominant mechanism causing their retreat. Two processes are responsible for bluff erosion and yield distinct bluff morphologies: (1) wave attack undercuts the bluff and causes episodic slumping, yielding planar bluff slopes, and (2) subaerial processes such as rainfall create irregular slopes characterized by rills and gullies. We propose a model of drumlin bluff evolution that is based on processes of erosion and physical characteristics such as bluff height, slope morphology, and the orientation of the bluff with respect to the long axis of the drumlin and its topographic crest. The four phases of drumlin bluff evolution consist of (1) initial formation of bluff, with retreat dominated by wave notching and slumping processes; (2) rill and gully development as bluff heights exceed 10 m and slumped sediment at bluff base inhibits wave attack; (3) return of wave notching and slumping as bluff heights decrease; and (4) final development of boulder retreat lag as last remnants of drumlin are eroded by wave action. These phases capture the important physical processes of drumlin evolution in Boston Harbor and could apply to other eroding coastal drumlin deposits.

Extrachromosomal deoxyribonucleic acid in R factor-harboring Enterobacteriaceae

DEFF Research Database (Denmark)

Møller, JK; Bak, AL; Christiansen, C

1976-01-01

Extrachromosomal deoxyribonucleic acid (DNA) from 24 different R factor-harboring Enterobacteriaceae was isolated and characterized by analytical ultracentrifugation and electron microscopy. The R factors represented 15 different patterns of transferable drug resistance found in enterobacteria from...

77 FR 46285 - Drawbridge Operation Regulation; Inner Harbor Navigational Canal, New Orleans, LA

Science.gov (United States)

2012-08-03

... Operation Regulation; Inner Harbor Navigational Canal, New Orleans, LA AGENCY: Coast Guard, DHS. ACTION... Navigational Canal, mile 3.1, at New Orleans, LA. The deviation is necessary to replace the wire rope lifting... of the US 90 (Danzinger) Bridge across the Inner Harbor Navigational Canal, mile 3.1, at New Orleans...

33 CFR 207.600 - Rochester (Charlotte) Harbor, N.Y.; use, administration, and navigation.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Rochester (Charlotte) Harbor, N.Y.; use, administration, and navigation. 207.600 Section 207.600 Navigation and Navigable Waters CORPS OF... (Charlotte) Harbor, N.Y.; use, administration, and navigation. (a)-(b) [Reserved] (c) No vessel shall moor or...

Ice Harbor Spillway Dissolved Gas Field Studies: Before and After Spillway Deflectors

Science.gov (United States)

2016-07-01

Steven C. Wilhelms Coastal And Hydraulics Laboratory U.S. Army Engineer Research and Development Center 3909 Halls Ferry Road Vicksburg, Mississippi...Harbor as a measure to reduce the total dissolved gas (TDG) production during spill operations. Three field studies were conducted at the Ice Harbor...significantly reduced for nearly all spill operations with deflectors in place. TDG near the stilling basin was reduced from approximately 150% to

Grays Harbor Paper

Energy Technology Data Exchange (ETDEWEB)

Quigg, B. [Grays Harbor Paper, Hoquiam, WA (United States)

2009-07-01

Wood waste biomass boilers are used at Grays Harbor Paper in Hoquiam, Washington. This presentation showed that large volumes of biomass are left after a traditional clearcut. The opportunities and challenges of collecting branches, tops and stumps from this wet coastal climate were outlined. The paper described some of the low-tech methods for picking up branches, stumps and woody debris. It included several photographs of custom logging machines for timber harvest, including a brush grapple slasher, a shearer shovel, chippers, grinders, slicesaws, trucks, trailers and caterpillar log loaders for handling slash. The slash recovery program relies on innovative harvesting machines that convert scattered logging slash into bundles that can be easily collected, transported, and stored for use in existing facilities that utilize wood fiber for fuel. figs.

Towards an ethics safe harbor for global biomedical research

Science.gov (United States)

Dove, Edward S.; Knoppers, Bartha M.; Zawati, Ma'n H.

2014-01-01

Although increasingly global, data-driven genomics and other ‘omics’-focused research hold great promise for health discoveries, current research ethics review systems around the world challenge potential improvements in human health from such research. To overcome this challenge, we propose a ‘Safe Harbor Framework for International Ethics Equivalency’ that facilitates the harmonization of ethics review of specific types of data-driven international research projects while respecting globally transposable research ethics norms and principles. The Safe Harbor would consist in part of an agency supporting an International Federation for Ethics Review (IFER), formed by a voluntary compact among countries, granting agencies, philanthropies, institutions, and healthcare, patient advocacy, and research organizations. IFER would be both a central ethics review body, and also a forum for review and follow-up of policies concerning ethics norms for international research projects. It would be built on five principle elements: (1) registration, (2) compliance review, (3) recognition, (4) monitoring and enforcement, and (5) public participation. The Safe Harbor would create many benefits for researchers, countries, and the general public, and may eventually have application beyond (gen)omics to other areas of biomedical research that increasingly engage in secondary use of data and present only negligible risks. PMID:27774154

77 FR 67563 - Regulated Navigation Area-New Haven Harbor, Quinnipiac River, Mill River, New Haven, CT; Pearl...

Science.gov (United States)

2012-11-13

... 1625-AA11 Regulated Navigation Area--New Haven Harbor, Quinnipiac River, Mill River, New Haven, CT... Haven Harbor, Quinnipiac River and Mill River. The current RNA pertains only to the operation of tugs...) entitled Regulated Navigation Area--New Haven Harbor, Quinnipiac River, Mill River, New Haven, CT; Pearl...

A smart ROV solution for ship hull and harbor inspection

Science.gov (United States)

Reed, Scott; Wood, Jon; Vazquez, Jose; Mignotte, Pierre-Yves; Privat, Benjamin

2010-04-01

Hull and harbor infrastructure inspections are frequently performed manually and involve quite a bit of risk and human and monetary resources. In any kind of threat and resource constrained environment, this involves unacceptable levels of risk and cost. Modern Remotely Operated Vehicles are highly refined machines that provide features and capabilities previously unavailable. Operations once carried out by divers can now be carried out more quickly, efficiently and safely by smart enabled ROVs. ROVs are rapidly deployable and capable of continuous, reliable operations in adverse conditions. They also provide a stable platform on which multiple sensors may be mounted and utilized to meet the harbor inspection problem. Automated Control software provides ROV's and their pilots with the capability to inspect complex, constrained environments such as those found in a harbor region. This application and the user interface allow the ROV to automatically conduct complex maneuvers relative to the area being inspected and relieves the training requirements and work load for the pilot, allowing he or she to focus on the primary task of survey, inspection and looking for possible threats (such as IEDs, Limpet Mines, signs of sabotage, etc). Real-time sensor processing tools can be integrated into the smart ROV solution to assist the operator. Automatic Target Recognition (ATR) algorithms are used to search through the sensor data collected by the ROV in real time. These algorithms provide immediate feedback on possible threats and notify the operator of regions that may require manual verification. Sensor data (sonar or video) is also mosaiced, providing the operator with real-time situational awareness and a coverage map of the hull or seafloor. Detected objects may also be placed in the context of the large scale characteristics of the hull (or bottom or pilings) and localized. Within the complex areas such as the harbor pier pilings and the running gear of the ship, real

Enhanced Magnetic Model 2015

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The Enhanced Magnetic Model (EMM) extends to degree and order 720, resolving magnetic anomalies down to 56 km wavelength. The higher resolution of the EMM results in...

Enhanced Magnetic Model 2010

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The Enhanced Magnetic Model (EMM) extends to degree and order 720, resolving magnetic anomalies down to 56 km wavelength. The higher resolution of the EMM results in...

75 FR 76613 - National Pearl Harbor Remembrance Day, 2010

Science.gov (United States)

2010-12-09

.... The deadly attack on Pearl Harbor did not accomplish its mission of breaking the American spirit..., aiding the war effort by working in manufacturing plants, participating in rationing programs, or...

Structural damage monitoring of harbor caissons with interlocking condition

Energy Technology Data Exchange (ETDEWEB)

Huynh, Thanh Canh; Lee, So Young; Nauyen, Khac Duy; Kim, Jeong Tae [Pukyong National Univ., Busan (Korea, Republic of)

2012-12-15

The objective of this study is to monitor the health status of harbor caissons which have potential foundation damage. To obtain the objective, the following approaches are performed. Firstly, a structural damage monitoring(SDM) method is designed for interlocked multiple caisson structures. The SDM method utilizes the change in modal strain energy to monitor the foundation damage in a target caisson unit. Secondly, a finite element model of a caisson system which consists of three caisson units is established to verify the feasibility of the proposed method. In the finite element simulation, the caisson units are constrained each other by shear key connections. The health status of the caisson system against various levels of foundation damage is monitored by measuring relative modal displacements between the adjacent caissons.

Structural damage monitoring of harbor caissons with interlocking condition

International Nuclear Information System (INIS)

Huynh, Thanh Canh; Lee, So Young; Nauyen, Khac Duy; Kim, Jeong Tae

2012-01-01

The objective of this study is to monitor the health status of harbor caissons which have potential foundation damage. To obtain the objective, the following approaches are performed. Firstly, a structural damage monitoring(SDM) method is designed for interlocked multiple caisson structures. The SDM method utilizes the change in modal strain energy to monitor the foundation damage in a target caisson unit. Secondly, a finite element model of a caisson system which consists of three caisson units is established to verify the feasibility of the proposed method. In the finite element simulation, the caisson units are constrained each other by shear key connections. The health status of the caisson system against various levels of foundation damage is monitored by measuring relative modal displacements between the adjacent caissons

Specific and Efficient Regression of Cancers Harboring KRAS Mutation by Targeted RNA Replacement.

Science.gov (United States)

Kim, Sung Jin; Kim, Ju Hyun; Yang, Bitna; Jeong, Jin-Sook; Lee, Seong-Wook

2017-02-01

Mutations in the KRAS gene, which persistently activate RAS function, are most frequently found in many types of human cancers. Here, we proposed and verified a new approach against cancers harboring the KRAS mutation with high cancer selectivity and efficient anti-cancer effects based on targeted RNA replacement. To this end, trans-splicing ribozymes from Tetrahymena group I intron were developed, which can specifically target and reprogram the mutant KRAS G12V transcript to induce therapeutic gene activity in cells. Adenoviral vectors containing the specific ribozymes with downstream suicide gene were constructed and then infection with the adenoviruses specifically downregulated KRAS G12V expression and killed KRAS G12V-harboring cancer cells additively upon pro-drug treatment, but it did not affect the growth of wild-type KRAS-expressing cells. Minimal liver toxicity was noted when the adenoviruses were administered systemically in vivo. Importantly, intratumoral injection of the adenoviruses with pro-drug treatment specifically and significantly impeded the growth of xenografted tumors harboring KRAS G12V through a trans-splicing reaction with the target RNA. In contrast, xenografted tumors harboring wild-type KRAS were not affected by the adenoviruses. Therefore, RNA replacement with a mutant KRAS-targeting trans-splicing ribozyme is a potentially useful therapeutic strategy to combat tumors harboring KRAS mutation. Copyright © 2017 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.

Transcriptome profiling identifies genes and pathways deregulated upon floxuridine treatment in colorectal cancer cells harboring GOF mutant p53

Directory of Open Access Journals (Sweden)

Arindam Datta

2016-06-01

Full Text Available Mutation in TP53 is a common genetic alteration in human cancers. Certain tumor associated p53 missense mutants acquire gain-of-function (GOF properties and confer oncogenic phenotypes including enhanced chemoresistance. The colorectal cancers (CRC harboring mutant p53 are generally aggressive in nature and difficult to treat. To identify a potential gene expression signature of GOF mutant p53-driven acquired chemoresistance in CRC, we performed transcriptome profiling of floxuridine (FUdR treated SW480 cells expressing mutant p53R273H (GEO#: GSE77533. We obtained several genes differentially regulated between FUdR treated and untreated cells. Further, functional characterization and pathway analysis revealed significant enrichment of crucial biological processes and pathways upon FUdR treatment in SW480 cells. Our data suggest that in response to chemotherapeutics treatment, cancer cells with GOF mutant p53 can modulate key cellular pathways to withstand the cytotoxic effect of the drugs. The genes and pathways identified in the present study can be further validated and targeted for better chemotherapy response in colorectal cancer patients harboring mutant p53.

78 FR 13479 - Drawbridge Operation Regulation; New Haven Harbor, Quinnipiac and Mill Rivers, CT

Science.gov (United States)

2013-02-28

...-AA09 Drawbridge Operation Regulation; New Haven Harbor, Quinnipiac and Mill Rivers, CT AGENCY: Coast... regulations that govern the operation of three bridges across the Quinnipiac and Mill Rivers at New Haven...) entitled ``Drawbridge Operation Regulations New Haven Harbor, Quinnipiac and Mill Rivers,'' in the Federal...

Texture Enhanced Appearance Models

DEFF Research Database (Denmark)

Larsen, Rasmus; Stegmann, Mikkel Bille; Darkner, Sune

2007-01-01

Statistical region-based registration methods such as the Active Appearance Model (AAM) are used for establishing dense correspondences in images. At low resolution, images correspondences can be recovered reliably in real-time. However, as resolution increases this becomes infeasible due...... representation are superior to wavelet representations at high dimensionality-reduction rates. At low reduction rates an edge enhanced wavelet representation provides better segmentation accuracy than the full standard AAM model....

A solitary hyperfunctioning thyroid nodule harboring thyroid carcinoma: review of the literature

OpenAIRE

Mirfakhraee, Sasan; Mathews, Dana; Peng, Lan; Woodruff, Stacey; Zigman, Jeffrey M

2013-01-01

Hyperfunctioning nodules of the thyroid are thought to only rarely harbor thyroid cancer, and thus are infrequently biopsied. Here, we present the case of a patient with a hyperfunctioning thyroid nodule harboring thyroid carcinoma and, using MEDLINE literature searches, set out to determine the prevalence of and characteristics of malignant ?hot? nodules as a group. Historical, biochemical and radiologic characteristics of the case subjects and their nodules were compared to those in cases o...

Caffeine in Boston Harbor past and present, assessing its utility as a tracer of wastewater contamination in an urban estuary

Science.gov (United States)

Sites throughout Boston Harbor were analyzed for caffeine to assess its utility as a tracer in identifying sources of sanitary wastewater. Caffeine ranged from 15 ng/L in the outer harbor to a high of 185 ng/L in the inner harbor. Inner harbor concentrations were a result of comb...

76 FR 37005 - Safety Zone; Fan Pier Yacht Club Fireworks, Boston Harbor, Boston, MA

Science.gov (United States)

2011-06-24

...-AA00 Safety Zone; Fan Pier Yacht Club Fireworks, Boston Harbor, Boston, MA AGENCY: Coast Guard, DHS... Sector Boston Captain of the Port (COTP) Zone for the Fan Pier Yacht Club Fireworks display. This safety... Safety Zone; Fan Pier Yacht Club Fireworks, Boston Harbor, Boston, Massachusetts. (a) General. A...

33 CFR 110.235 - Pacific Ocean (Mamala Bay), Honolulu Harbor, Hawaii (Datum: NAD 83).

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Pacific Ocean (Mamala Bay), Honolulu Harbor, Hawaii (Datum: NAD 83). 110.235 Section 110.235 Navigation and Navigable Waters COAST... Pacific Ocean (Mamala Bay), Honolulu Harbor, Hawaii (Datum: NAD 83). (a) The anchorage grounds—(1...

33 CFR 165.708 - Safety/Security Zone; Charleston Harbor and Cooper River, Charleston, SC.

Science.gov (United States)

2010-07-01

... Harbor and Cooper River, Charleston, SC. 165.708 Section 165.708 Navigation and Navigable Waters COAST... Guard District § 165.708 Safety/Security Zone; Charleston Harbor and Cooper River, Charleston, SC. (a... Cooper River. All coordinates referenced use datum: NAD 1983. (2) All waters within 100 yards of the...

77 FR 42076 - Notice of Opportunity for Public Comment on Surplus Property Release at Hancock County-Bar Harbor...

Science.gov (United States)

2012-07-17

... Comment on Surplus Property Release at Hancock County-Bar Harbor Airport, Trenton, ME AGENCY: Federal... located at Hancock County-Bar Harbor Airport, Trenton, Maine. DATES: Comments must be received on or... INFORMATION: The Federal Aviation Administration is reviewing a request by Hancock County-Bar Harbor Airport...

Hydrocarbon pollutants shape bacterial community assembly of harbor sediments

KAUST Repository

Barbato, Marta; Mapelli, Francesca; Magagnini, Mirko; Chouaia, Bessem; Armeni, Monica; Marasco, Ramona; Crotti, Elena; Daffonchio, Daniele; Borin, Sara

2016-01-01

Petroleum pollution results in co-contamination by different classes of molecules, entailing the occurrence of marine sediments difficult to remediate, as in the case of the Ancona harbor (Mediterranean Sea, Italy). Autochthonous bioaugmentation

Submarine Biofouling Control- Chlorination DATS Study at Pearl Harbor

National Research Council Canada - National Science Library

Wegand, John

2001-01-01

The intent of this document is to sumarize the chlorination studies performed at Naval Station, Pearl Harbor in support of biofouling control initiatives for the submarine community, as requested by NAVSEA 92T...

77 FR 38490 - Safety Zone; Mentor Harbor Yachting Club Fireworks, Lake Erie, Mentor, OH

Science.gov (United States)

2012-06-28

...-AA00 Safety Zone; Mentor Harbor Yachting Club Fireworks, Lake Erie, Mentor, OH AGENCY: Coast Guard, DHS... Erie, Mentor, OH. This safety zone is intended to restrict vessels from a portion of Lake Erie during the Mentor Harbor Yachting Club fireworks display. This temporary safety zone is necessary to protect...

Human case of bacteremia caused by Streptococcus canis sequence type 9 harboring the scm gene.

Science.gov (United States)

Taniyama, Daisuke; Abe, Yoshihiko; Sakai, Tetsuya; Kikuchi, Takahide; Takahashi, Takashi

2017-01-01

Streptococcus canis (Sc) is a zoonotic pathogen that is transferred mainly from companion animals to humans. One of the major virulence factors in Sc is the M-like protein encoded by the scm gene, which is involved in anti-phagocytic activities, as well as the recruitment of plasminogen to the bacterial surface in cooperation with enolase, and the consequent enhancement of bacterial transmigration and survival. This is the first reported human case of uncomplicated bacteremia following a dog bite, caused by Streptococcus canis harboring the scm gene. The similarity of the 16S rRNA from the infecting species to that of the Sc type strain, as well as the amplification of the species-specific cfg gene, encoding a co-hemolysin, was used to confirm the species identity. Furthermore, the isolate was confirmed as sequence type 9. The partial scm gene sequence harbored by the isolate was closely related to those of other two Sc strains. While this isolate did not possess the erm (A), erm (B), or mef (A), macrolide/lincosamide resistance genes, it was not susceptible to azithromycin: its susceptibility was intermediate. Even though human Sc bacteremia is rare, clinicians should be aware of this microorganism, as well as Pasteurella sp., Prevotella sp., and Capnocytophaga sp., when examining and treating patients with fever who maintain close contact with companion animals.

Cell fusion phenomena detected after in utero transplantation of Ds-red-harboring porcine amniotic fluid stem cells into EGFP transgenic mice.

Science.gov (United States)

Peng, Shao-Yu; Chen, Yu-Hsu; Chou, Chih-Jen; Wang, Yao-Horng; Lee, Hung-Maan; Cheng, Winston Teng-Kui; Shaw, S W Steven; Wu, Shinn-Chih

2014-05-01

Amniotic fluid stem cells (AFSCs) are derived from the amniotic fluid of the developing fetus and can give rise to diverse differentiated cells of ectoderm, mesoderm, and endoderm lineages. Intrauterine transplantation is an approach used to cure inherited genetic fetal defects during the gestation period of pregnant dams. Certain disease such as osteogenesis imperfecta was successfully treated in affected fetal mice using this method. However, the donor cell destiny remains uncertain. The purpose of this study was to investigate the biodistribution and cell fate of Ds-red-harboring porcine AFSCs (Ds-red pAFSCs) after intrauterine transplantation into enhanced green fluorescent protein-harboring fetuses of pregnant mice. Pregnant mice (12.5 days) underwent open laparotomy with intrauterine pAFSC transplantation (5 × 10(4) cells per pup) into fetal peritoneal cavity. Three weeks after birth, the mice were sacrificed. Several samples from different organs were obtained for histological examination and flow cytometric analysis. Ds-red pAFSCs migrated most frequently into the intestines. Furthermore, enhanced green fluorescent protein and red fluorescent protein signals were co-expressed in the intestine and liver cells via immunohistochemistry studies. In utero xenotransplantation of pAFSCs fused with recipient intestinal cells instead of differentiating or maintaining the undifferentiated status in the tissue. © 2014 John Wiley & Sons, Ltd.

A Comprehensive Copper Compliance Strategy: Implementing Regulatory Guidance at Pearl Harbor Naval Shipyard & Intermediate Maintenance Facility

National Research Council Canada - National Science Library

Earley, P. J; Rosen, G; Rivera-Duarte, I; Gauthier, R. D; Arias-Thode, Y; Thompson, J; Swope, B

2007-01-01

Studies were performed to develop a new National Pollution Discharge Elimination Systems Permit for the discharge of effluents from the Pearl Harbor Naval Shipyard and Intermediate Maintenance Facility into Pearl Harbor...

76 FR 38302 - Safety Zone; Cape Charles Fireworks, Cape Charles Harbor, Cape Charles, VA

Science.gov (United States)

2011-06-30

... the Town of Cape Charles will sponsor a fireworks display on the shoreline of the navigable waters of...-AA00 Safety Zone; Cape Charles Fireworks, Cape Charles Harbor, Cape Charles, VA AGENCY: Coast Guard... navigable waters of Cape Charles City Harbor in Cape Charles, VA in support of the Fourth of July Fireworks...

76 FR 71598 - Pearl Harbor National Wildlife Refuge, Honolulu County, HI; Final Comprehensive Conservation Plan...

Science.gov (United States)

2011-11-18

...] Pearl Harbor National Wildlife Refuge, Honolulu County, HI; Final Comprehensive Conservation Plan and.../docs/HI-PI/docsjcpearl.htm . Email: [email protected] . Include ``Pearl Harbor final CCP'' in...`iwa, HI 96712. FOR FURTHER INFORMATION CONTACT: David Ellis, Project Leader, (808) 637-6330...

Impact of Geomorphological Changes to Harbor Resonance During Meteotsunamis: The Vela Luka Bay Test Case

Science.gov (United States)

Denamiel, Cléa; Šepić, Jadranka; Vilibić, Ivica

2018-05-01

In engineering studies, harbor resonance, including quality and amplification factors, is typically computed for swell and waves with periods shorter than 10 min. However, in various locations around the world, such as Vela Luka Bay in Croatia, meteotsunami waves of periods greater than 10 min can excite the bay or harbor natural modes and produce substantial structural damages. In this theoretical study, the impact of some geomorphological changes of Vela Luka Bay—i.e. deepening of the bay, dredging the harbor, adding a pier or a marina—to the amplification of the meteotsunami waves are presented for a set of 6401 idealized pressure wave field forcing used to derive robust statistics. The most substantial increase in maximum elevation is found when the Vela Luka harbor is dredged to a 5 m depth, which is in contradiction with the calculation of the quality factor showing a decrease of the harbor natural resonance. It has been shown that the forcing energy content at different frequency bands should also be taken into account when estimating the quality and amplification factors, as their typical definitions derived from the peak frequency of the sea level spectrum fail to represent the harbor response during meteotsunami events. New definitions of these factors are proposed in this study and are shown to be in good agreement with the results of the statistical analysis of the Vela Luka Bay maximum elevation results. In addition, the presented methodology can easily be applicable to any other location in the world where meteotsunamis occur.

77 FR 24381 - Security Zone; Passenger Vessel SAFARI EXPLORER Arrival/Departure, Kaunakakai Harbor, Molokai, HI

Science.gov (United States)

2012-04-24

...-AA87 Security Zone; Passenger Vessel SAFARI EXPLORER Arrival/ Departure, Kaunakakai Harbor, Molokai, HI... channel's entrance during the arrival and departure of the Passenger Vessel Safari Explorer in Kaunakakai... entrance during the arrival and departure of the Passenger Vessel Safari Explorer in Kaunakakai Harbor...

A lifelong journey of moving beyond wartime trauma for survivors from Hiroshima and Pearl Harbor.

Science.gov (United States)

Liehr, Patricia; Nishimura, Chie; Ito, Mio; Wands, Lisa Marie; Takahashi, Ryutaro

2011-01-01

This study examines 51 stories of health, shared by people who survived the wartime trauma of Hiroshima and Pearl Harbor, seeking to identify turning points that moved participants along over their lifetime. The central turning point for Hiroshima survivors was "becoming Hibabusha (A-bomb survivor)" and for Pearl Harbor survivors was "honoring the memory and setting it aside." Wartime trauma was permanently integrated into survivors' histories, surfacing steadily over decades for Hiroshima survivors and intermittently over decades for Pearl Harbor survivors. Regardless of experience or nationality, participants moved through wartime trauma by connecting with others, pursuing personal and global peace.

Temporal and spatial variation in harbor seal (Phoca vitulina L.) roar calls from southern Scandinavia

DEFF Research Database (Denmark)

Sabinsky, Puk Faxe; Larsen, Ole Næsbye; Wahlberg, Magnus

2017-01-01

Male harbor seals gather around breeding sites for competitive mating displays. Here, they produce underwater vocalizations possibly to attract females and/or scare off other males. These calls offer prospects for passive acoustic monitoring. Acoustic monitoring requires a good understanding...... of natural variation in calling behavior both temporally and among geographically separate sites. Such variation in call structure and calling patterns were studied in harbor seal vocalizations recorded at three locations in Danish and Swedish waters. There was a strong seasonality in the calls from end...... biological differences when comparing harbor seal roars among recording sites and between years....

Emergence of colistin-resistant Escherichia coli clinical isolates harboring mcr-1 in Vietnam.

Science.gov (United States)

Tada, Tatsuya; Nhung, Pham Hong; Shimada, Kayo; Tsuchiya, Mitsuhiro; Phuong, Doan Mai; Anh, Nguyen Quoc; Ohmagari, Norio; Kirikae, Teruo

2017-10-01

The mcr-1 was first detected on a plasmid in colistin-resistant Escherichia coli from livestock and patients in China. We described here the emergence of colistin-resistant E. coli clinical isolates harboring mcr-1 on the chromosomes in Vietnam. To our knowledge, this is the first report of hospital-acquired E. coli isolates harboring mcr-1 in a medical setting in Vietnam. Copyright © 2017. Published by Elsevier Ltd.

An Evaluation of the Acquisition Streamlining Methods at the Fleet and Industrial Supply Center Pearl Harbor Hawaii

National Research Council Canada - National Science Library

Henry, Mark

1999-01-01

...) Pearl Harbor's implementation of acquisition streamlining initiatives and recommends viable methods of streamlining the acquisition process at FISC Pearl Harbor and other Naval Supply Systems Command...

Quantitative Measures of Anthropogenic Noise on Harbor Porpoises

DEFF Research Database (Denmark)

Wisniewska, Danuta Maria; Teilmann, Jonas; Hermannsen, Line

2016-01-01

-animal recordings as proxies for actual exposure. Here, we quantify sound exposure levels recorded with a DTAG-3 tag on a captive harbor porpoise exposed to vessel noise in a controlled acoustic environment. Results show that fl ow noise is limiting onboard noise recordings, whereas no evidence of body shading has...

77 FR 2019 - Security Zone; Passenger Vessel SAFARI EXPLORER Arrival/Departure, Kaunakakai Harbor, Molokai, HI

Science.gov (United States)

2012-01-13

...-AA87 Security Zone; Passenger Vessel SAFARI EXPLORER Arrival/ Departure, Kaunakakai Harbor, Molokai, HI... vessel SAFARI EXPLORER to its intended berth in the harbor. Entry into the temporary security zone is... operation of the SAFARI EXPLORER into Molokai has been voluntarily suspended by the operating company...

78 FR 19632 - Special Local Regulations; St. Thomas Carnival Watersport Activities, Charlotte Amalie Harbor; St...

Science.gov (United States)

2013-04-02

...-AA08 Special Local Regulations; St. Thomas Carnival Watersport Activities, Charlotte Amalie Harbor; St... proposes to establish a special local regulation on the waters of Charlotte Amalie Harbor in St Thomas, USVI during the St. Thomas Carnival Watersport Activities, a high speed boat race. The event is...

Virulence of Klebsiella pneumoniae isolates harboring bla KPC-2 carbapenemase gene in a Caenorhabditis elegans model.

Directory of Open Access Journals (Sweden)

Jean-Philippe Lavigne

Full Text Available Klebsiella pneumoniae carbapenemase (KPC is a carbapenemase increasingly reported worldwide in Enterobacteriaceae. The aim of this study was to analyze the virulence of several KPC-2-producing K. pneumoniae isolates. The studied strains were (i five KPC-2 clinical strains from different geographical origins, belonging to different ST-types and possessing plasmids of different incompatibility groups; (ii seven transformants obtained after electroporation of either these natural KPC plasmids or a recombinant plasmid harboring only the bla KPC-2 gene into reference strains K. pneumoniae ATCC10031/CIP53153; and (iii five clinical strains cured of plasmids. The virulence of K. pneumoniae isolates was evaluated in the Caenorhabditis elegans model. The clinical KPC producers and transformants were significantly less virulent (LT50: 5.5 days than K. pneumoniae reference strain (LT50: 4.3 days (p<0.01. However, the worldwide spread KPC-2 positive K. pneumoniae ST258 strains and reference strains containing plasmids extracted from K. pneumoniae ST258 strains had a higher virulence than KPC-2 strains belonging to other ST types (LT50: 5 days vs. 6 days, p<0.01. The increased virulence observed in cured strains confirmed this trend. The bla KPC-2 gene itself was not associated to increased virulence.

Discrete event simulation for petroleum transfers involving harbors, refineries and pipelines

Energy Technology Data Exchange (ETDEWEB)

Martins, Marcella S.R.; Lueders, Ricardo; Delgado, Myriam R.B.S. [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil)

2009-07-01

Nowadays a great effort has been spent by companies to improve their logistics in terms of programming of events that affect production and distribution of products. In this case, simulation can be a valuable tool for evaluating different behaviors. The objective of this work is to build a discrete event simulation model for scheduling of operational activities in complexes containing one harbor and two refineries interconnected by a pipeline infrastructure. The model was developed in Arena package, based on three sub-models that control pier allocation, loading of tanks, and transfers to refineries through pipelines. Preliminary results obtained for a given control policy, show that profit can be calculated by taking into account many parameters such as oil costs on ships, pier using, over-stay of ships and interface costs. Such problem has already been considered in the literature but using different strategies. All these factors should be considered in a real-world operation where decision making tools are necessary to obtain high returns. (author)

Analysis of Protection Measures for Naval Vessels Berthed at Harbor Against Terrorist Attacks

Science.gov (United States)

2016-06-01

of discriminating neutral vessels from threats. A naval vessel berthed at harbor is more susceptible to attack than a vessel in open seas. The...discriminating neutral vessels from threats. A naval vessel berthed at harbor is more susceptible to attack than a vessel in open seas. The chances of...this thesis. He was a source of inspiration, encouragement, and reassurance. Captain Jeffery E. Kline, I am really thankful to you for your ideas

Cladophora (Chlorophyta) spp. Harbor Human Bacterial Pathogens in Nearshore Water of Lake Michigan†

OpenAIRE

Ishii, Satoshi; Yan, Tao; Shively, Dawn A.; Byappanahalli, Muruleedhara N.; Whitman, Richard L.; Sadowsky, Michael J.

2006-01-01

Cladophora glomerata, a macrophytic green alga, is commonly found in the Great Lakes, and significant accumulations occur along shorelines during the summer months. Recently, Cladophora has been shown to harbor high densities of the fecal indicator bacteria Escherichia coli and enterococci. Cladophora may also harbor human pathogens; however, until now, no studies to address this question have been performed. In the present study, we determined whether attached Cladophora, obtained from the L...

Calculating background levels for ecological risk parameters in toxic harbor sediment

Science.gov (United States)

Leadon, C.J.; McDonnell, T.R.; Lear, J.; Barclift, D.

2007-01-01

Establishing background levels for biological parameters is necessary in assessing the ecological risks from harbor sediment contaminated with toxic chemicals. For chemicals in sediment, the term contaminated is defined as having concentrations above background and significant human health or ecological risk levels. For biological parameters, a site could be considered contaminated if levels of the parameter are either more or less than the background level, depending on the specific parameter. Biological parameters can include tissue chemical concentrations in ecological receptors, bioassay responses, bioaccumulation levels, and benthic community metrics. Chemical parameters can include sediment concentrations of a variety of potentially toxic chemicals. Indirectly, contaminated harbor sediment can impact shellfish, fish, birds, and marine mammals, and human populations. This paper summarizes the methods used to define background levels for chemical and biological parameters from a survey of ecological risk investigations of marine harbor sediment at California Navy bases. Background levels for regional biological indices used to quantify ecological risks for benthic communities are also described. Generally, background stations are positioned in relatively clean areas exhibiting the same physical and general chemical characteristics as nearby areas with contaminated harbor sediment. The number of background stations and the number of sample replicates per background station depend on the statistical design of the sediment ecological risk investigation, developed through the data quality objective (DQO) process. Biological data from the background stations can be compared to data from a contaminated site by using minimum or maximum background levels or comparative statistics. In Navy ecological risk assessments (ERA's), calculated background levels and appropriate ecological risk screening criteria are used to identify sampling stations and sites with contaminated

33 CFR 165.T14-204 - Safety Zone; fixed mooring balls, south of Barbers Pt Harbor Channel, Oahu, Hawaii.

Science.gov (United States)

2010-07-01

..., south of Barbers Pt Harbor Channel, Oahu, Hawaii. 165.T14-204 Section 165.T14-204 Navigation and... Pt Harbor Channel, Oahu, Hawaii. (a) Location. The following area is a safety zone: All waters... position is approximately 2,500 yards south of Barbers Point Harbor channel buoy #2, Oahu, Hawaii. This...

U.S. Department of Defense - Pearl Harbor Special

Science.gov (United States)

Hawaii saw that seminal moment in history, and those that were there vividly remember that Sunday morning this noise," he said in an oral history on the Pearl Harbor Survivors' Association Web site. " seeing was an attack. "It didn't mean anything to us until a large group of planes came near the

Wedgelet Enhanced Appearance Models

DEFF Research Database (Denmark)

Darkner, Sune; Larsen, Rasmus; Stegmann, Mikkel Bille

2004-01-01

Statistical region-based segmentation methods such as the Active Appearance Model (AAM) are used for establishing dense correspondences in images based on learning the variation in shape and pixel intensities in a training set. For low resolution 2D images correspondences can be recovered reliably....... The wedgelet regression trees employed are based on triangular domains and estimated using cross validation. The wedgelet regression trees are functional descriptions of the intensity information and serve to 1) reduce noise and 2) produce a compact textural description. The wedgelet enhanced appearance model...

The Plurality of Harbors at Caesarea: The Southern Anchorage in Late Antiquity

Science.gov (United States)

Ratzlaff, Alexandra; Galili, Ehud; Waiman-Barak, Paula; Yasur-Landau, Assaf

2017-08-01

The engineering marvel of Sebastos, or Portus Augusti as it was called in Late Antiquity (284-638 CE), dominated Caesarea's harbor center along modern Israel's central coast but it was only one part of a larger maritime complex. The Southern Anchorage provides a case study as one portion of the Caesarea complex, as well as a node within the regional network of anchorages and small harbors. Ceramics recovered from here show a high percentage of locally, and provincially, produced storage jars engaged in maritime trade. The ceramic evidence points towards an intensified regional trade or cabotage rather than favouring long distance trade from large port to port. Working out of these small harbors, opportunities arose for greater flexibility in specialization of commodities and materials passing through the network of subsidiary ports, contributing to a more diversified market economy. This analysis provides another example in the growing focus on how these simple and semi-modified anchorages in the Eastern Mediterranean were often the predominant economic networks connecting hinterland and coastal trade.

Length-weight relationship of northern pike, Esox lucius, from East Harbor, Ohio

Science.gov (United States)

Brown, Edward H.; Clark, Clarence F.

1965-01-01

The northern pike is one of Ohio's largest game fish but is well known to comparatively few anglers. Large numbers of the big fish spawn in the Ohio marshes adjacent to Lake Erie. Movements related to spawning reach a peak in late March or early April. Later the spawning population disperses and is seldom represented in catches by experimental gear or by anglers. The short period of availability was used to obtain life history information in March of 1951 through 1953. No comprehensive length-weight data for this species have previously been published from this area. East Harbor is a sandspit pond separated from Lake Erie by a large sand bar. Waters and fish populations of the harbor and lake can mix freely through a permanent connecting channel. The larger part of the 850 surface acres of the harbor is normally less than 8 feet deep. The male northern pike averaged 20.5 inches in length and ranged from 13.5 to 28.5 inches. The conspicuously larger females averaged 26.0 inches and ranged from 15.5 to 37.5 inches.

77 FR 60109 - Takes of Marine Mammals Incidental to Specified Activities; Harbor Activities Related to the...

Science.gov (United States)

2012-10-02

...; and Northern elephant seals by Level B harassment only. To date, we have issued nine, 1-year... numbers of marine mammals, incidental to conducting Delta Mariner harbor operations for one year. We... California sea lions (Zalophus californianus), Pacific harbor seals (Phoca vitulina), and Northern elephant...

Enhanced index tracking modelling in portfolio optimization

Science.gov (United States)

Lam, W. S.; Hj. Jaaman, Saiful Hafizah; Ismail, Hamizun bin

2013-09-01

Enhanced index tracking is a popular form of passive fund management in stock market. It is a dual-objective optimization problem, a trade-off between maximizing the mean return and minimizing the risk. Enhanced index tracking aims to generate excess return over the return achieved by the index without purchasing all of the stocks that make up the index by establishing an optimal portfolio. The objective of this study is to determine the optimal portfolio composition and performance by using weighted model in enhanced index tracking. Weighted model focuses on the trade-off between the excess return and the risk. The results of this study show that the optimal portfolio for the weighted model is able to outperform the Malaysia market index which is Kuala Lumpur Composite Index because of higher mean return and lower risk without purchasing all the stocks in the market index.

Enhancements to ASHRAE Standard 90.1 Prototype Building Models

Energy Technology Data Exchange (ETDEWEB)

Goel, Supriya; Athalye, Rahul A.; Wang, Weimin; Zhang, Jian; Rosenberg, Michael I.; Xie, YuLong; Hart, Philip R.; Mendon, Vrushali V.

2014-04-16

This report focuses on enhancements to prototype building models used to determine the energy impact of various versions of ANSI/ASHRAE/IES Standard 90.1. Since the last publication of the prototype building models, PNNL has made numerous enhancements to the original prototype models compliant with the 2004, 2007, and 2010 editions of Standard 90.1. Those enhancements are described here and were made for several reasons: (1) to change or improve prototype design assumptions; (2) to improve the simulation accuracy; (3) to improve the simulation infrastructure; and (4) to add additional detail to the models needed to capture certain energy impacts from Standard 90.1 improvements. These enhancements impact simulated prototype energy use, and consequently impact the savings estimated from edition to edition of Standard 90.1.

Haul-out behavior of harbor seals (Phoca vitulina in Hood Canal, Washington.

Directory of Open Access Journals (Sweden)

Josh M London

Full Text Available The goal of this study was to model haul-out behavior of harbor seals (Phoca vitulina in the Hood Canal region of Washington State with respect to changes in physiological, environmental, and temporal covariates. Previous research has provided a solid understanding of seal haul-out behavior. Here, we expand on that work using a generalized linear mixed model (GLMM with temporal autocorrelation and a large dataset. Our dataset included behavioral haul-out records from archival and VHF radio tag deployments on 25 individual seals representing 61,430 seal hours. A novel application for increased computational efficiency allowed us to examine this large dataset with a GLMM that appropriately accounts for temporal autocorellation. We found significant relationships with the covariates hour of day, day of year, minutes from high tide and year. Additionally, there was a significant effect of the interaction term hour of day : day of year. This interaction term demonstrated that seals are more likely to haul out during nighttime hours in August and September, but then switch to predominantly daylight haul-out patterns in October and November. We attribute this change in behavior to an effect of human disturbance levels. This study also examined a unique ecological event to determine the role of increased killer whale (Orcinus orca predation on haul-out behavior. In 2003 and 2005 these harbor seals were exposed to unprecedented levels of killer whale predation and results show an overall increase in haul-out probability after exposure to killer whales. The outcome of this study will be integral to understanding any changes in population abundance as a result of increased killer whale predation.

Sediment quality in Burlington Harbor, Lake Champlain, U.S.A.

Science.gov (United States)

Lacey, E.M.; King, J.W.; Quinn, J.G.; Mecray, E.L.; Appleby, P.G.; Hunt, A.S.

2001-01-01

Surface samples and cores were collected in 1993 from the Burlington Harbor region of Lake Champlain. Sediment samples were analyzed for trace metals (cadmium, copper, lead, nickel, silver and zinc), simultaneously extracted metal/acid volatile sulfide (SEM-AVS), grain size, nutrients (carbon and nitrogen) and organic contaminants (polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs)). The concentrations of cadmium, copper, silver and zinc from the partial sediment digestion of the surface samples correlated well with each other (r2 > 0.60) indicating that either a common process, or group of processes determined the sediment concentrations of these metals. In an analysis of the spatial distribution of the trace metals and PAHs, high surficial concentrations were present in the southern portion of the Harbor. The trace metal trend was strengthened when the concentrations were normalized by grain size. A sewage treatment plant outfall discharge was present in the southeastern portion of the Harbor at the time of this study and is the major source of trace metal and PAH contamination. Evaluation of sediment cores provides a proxy record of historical trace metal and organic inputs. The peak accumulation rate for copper, cadmium, lead, and zinc was in the late 1960s and the peak silver accumulation rate was later. The greatest accumulation of trace metals occurred in the late 1960s after discharges from the STP began. Subsequent declines in trace metal concentrations may be attributed to increased water and air regulations. The potential toxicity of trace metals and organic contaminants was predicted by comparing contaminant concentrations to benchmark concentrations and potential trace metal bioavailability was predicted with SEM-AVS results. Surface sample results indicate lead, silver, ???PAHs and ???PCBs are potentially toxic and/or bioavailable. These predictions were supported by studies of biota in the Burlington Harbor watershed. There

Enhanced production of recombinant proteins with Corynebacterium glutamicum by deletion of insertion sequences (IS elements).

Science.gov (United States)

Choi, Jae Woong; Yim, Sung Sun; Kim, Min Jeong; Jeong, Ki Jun

2015-12-29

In most bacteria, various jumping genetic elements including insertion sequences elements (IS elements) cause a variety of genetic rearrangements resulting in harmful effects such as genome and recombinant plasmid instability. The genetic stability of a plasmid in a host is critical for high-level production of recombinant proteins, and in this regard, the development of an IS element-free strain could be a useful strategy for the enhanced production of recombinant proteins. Corynebacterium glutamicum, which is a workhorse in the industrial-scale production of various biomolecules including recombinant proteins, also has several IS elements, and it is necessary to identify the critical IS elements and to develop IS element deleted strain. From the cultivation of C. glutamicum harboring a plasmid for green fluorescent protein (GFP) gene expression, non-fluorescent clones were isolated by FACS (fluorescent activated cell sorting). All the isolated clones had insertions of IS elements in the GFP coding region, and two major IS elements (ISCg1 and ISCg2 families) were identified. By co-cultivating cells harboring either the isolated IS element-inserted plasmid or intact plasmid, it was clearly confirmed that cells harboring the IS element-inserted plasmids became dominant during the cultivation due to their growth advantage over cells containing intact plasmids, which can cause a significant reduction in recombinant protein production during cultivation. To minimize the harmful effects of IS elements on the expression of heterologous genes in C. glutamicum, two IS element free C. glutamicum strains were developed in which each major IS element was deleted, and enhanced productivity in the engineered C. glutamicum strain was successfully demonstrated with three models: GFP, poly(3-hydroxybutyrate) [P(3HB)] and γ-aminobutyrate (GABA). Our findings clearly indicate that the hopping of IS elements could be detrimental to the production of recombinant proteins in C

78 FR 28492 - Special Local Regulation; Low Country Splash, Wando River, Cooper River, and Charleston Harbor...

Science.gov (United States)

2013-05-15

...-AA08 Special Local Regulation; Low Country Splash, Wando River, Cooper River, and Charleston Harbor... establishing a special local regulation on the waters of the Wando River, Cooper River, and Charleston Harbor... rulemaking (NPRM) entitled, ``Special Local Regulation; Low Country Splash, Wando River, Cooper River, and...

78 FR 21597 - Marine Mammals: Alaska Harbor Seal Habitats

Science.gov (United States)

2013-04-11

... seek input as to whether management measures are needed, and if so, what types of measures should be... proposed rulemaking (ANPR) on potential management measures to protect glacially-associated harbor seal... need for regulations; (2) the geographic scope and time horizon of regulations; (3) management options...

Hydrocarbon pollutants shape bacterial community assembly of harbor sediments

KAUST Repository

Barbato, Marta

2016-02-02

Petroleum pollution results in co-contamination by different classes of molecules, entailing the occurrence of marine sediments difficult to remediate, as in the case of the Ancona harbor (Mediterranean Sea, Italy). Autochthonous bioaugmentation (ABA), by exploiting the indigenous microbes of the environment to be treated, could represent a successful bioremediation strategy. In this perspective we aimed to i) identify the main drivers of the bacterial communities\\' richness in the sediments, ii) establish enrichment cultures with different hydrocarbon pollutants evaluating their effects on the bacterial communities\\' composition, and iii) obtain a collection of hydrocarbon degrading bacteria potentially exploitable in ABA. The correlation between the selection of different specialized bacterial populations and the type of pollutants was demonstrated by culture-independent analyses, and by establishing a collection of bacteria with different hydrocarbon degradation traits. Our observations indicate that pollution dictates the diversity of sediment bacterial communities and shapes the ABA potential in harbor sediments.

Gridded multibeam bathymetry of Apra Harbor, Guam U.S. Territory

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Gridded bathymetry from Apra Harbor, Guam U.S. Territory. The netCDF and Arc ASCII grids include multibeam bathymetry from the Reson SeaBat 8125 multibeam sonar...

Grays Harbor and Chehalis River Improvements to Navigation Environmental Studies. Wildlife Studies at Proposed Disposal Sites in Grays Harbor, Washington,

Science.gov (United States)

1982-01-01

sltand. T 𔃼~P i’ W 210 three times VtwCerI November IOC’C -nd ~co l.Etls ~ ec!,!zervc-o betxwe H -gF 12 Th -ind hl rway u- 7Plie Sicuobh. E. Cumin -s 1... stress imposed by dredge dsosal ;ictivities on these species. It is difficult to rredict the effects of establishing a salt marsh in Grays Harbor on

Composition and source of butyltins in sediments of Kaohsiung Harbor, Taiwan

Science.gov (United States)

Dong, Cheng-Di; Chen, Chih-Feng; Chen, Chiu-Wen

2015-04-01

Fifty-eight sediment samples were collected from the Kaohsiung Harbor (Taiwan) for analyses of monobutyltin (MBT), dibutyltin (DBT) and tributyltin (TBT), using gas chromatography/flame photometric detector (GC/FPD). The concentration of total butyltins (ΣBTs), sum of MBT, DBT, and TBT, varied from 3.9 to 158.5 ng Sn/g dw in sediment samples with TBT being the major component of the sediment samples, except for the vicinity of the Love River mouth where MBT was the most abundant BT compound (a proportion of over 57%). Based on the BTs concentration, distribution, composition and correlations, the sources of BTs found in harbor sediments are shipping activities, and TBT is the main pollutant; the estuary (i.e. Love River) has been the anthropogenic source of MBT from upstream inputs. Influences of TBT on aquatic organisms are evaluated using the toxicity guidelines proposed by the US EPA (US Environmental Protection Agency) and the ACCI (assessment class criterion for imposex) proposed by OSPAR (Oslo and Paris Commission). The evaluation shows that the TBT contained in the sediment at Kaohsiung Harbor is likely to have a negative influence at ACCI class C because gastropods present imposex and TBT levels are above ecotoxicological assessment criteria (EAC) limits.

Model based Binaural Enhancement of Voiced and Unvoiced Speech

DEFF Research Database (Denmark)

Kavalekalam, Mathew Shaji; Christensen, Mads Græsbøll; Boldt, Jesper B.

2017-01-01

This paper deals with the enhancement of speech in presence of non-stationary babble noise. A binaural speech enhancement framework is proposed which takes into account both the voiced and unvoiced speech production model. The usage of this model in enhancement requires the Short term predictor...... (STP) parameters and the pitch information to be estimated. This paper uses a codebook based approach for estimating the STP parameters and a parametric binaural method is proposed for estimating the pitch parameters. Improvements in objective score are shown when using the voicedunvoiced speech model...

33 CFR 162.260 - Channel leading to San Juan Harbor, P.R.; use, administration, and navigation.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Channel leading to San Juan Harbor, P.R.; use, administration, and navigation. 162.260 Section 162.260 Navigation and Navigable... WATERWAYS NAVIGATION REGULATIONS § 162.260 Channel leading to San Juan Harbor, P.R.; use, administration...

DefenseLink Special: Attack on Pearl Harbor, 65th Anniversary

Science.gov (United States)

Japanese raid on Pearl Harbor has been called a defining moment in U.S. history. It caught the country by Hawaii-based combat planes, were heavily damaged. By crippling the U.S. Pacific Fleet, Japan hoped to

Abstracts of papers presented at the LVIII Cold Spring Harbor Symposium on quantitative Biology: DNA and chromosomes

Energy Technology Data Exchange (ETDEWEB)

1993-12-31

This volume contains the abstracts of oral and poster presentations made at the LVIII Cold Spring Harbor Symposium on Quantitative Biology entitles DNA & Chromosomes. The meeting was held June 2--June 9, 1993 at Cold Spring Harbor, New York.

Dynamic modeling method for infrared smoke based on enhanced discrete phase model

Science.gov (United States)

Zhang, Zhendong; Yang, Chunling; Zhang, Yan; Zhu, Hongbo

2018-03-01

The dynamic modeling of infrared (IR) smoke plays an important role in IR scene simulation systems and its accuracy directly influences the system veracity. However, current IR smoke models cannot provide high veracity, because certain physical characteristics are frequently ignored in fluid simulation; simplifying the discrete phase as a continuous phase and ignoring the IR decoy missile-body spinning. To address this defect, this paper proposes a dynamic modeling method for IR smoke, based on an enhanced discrete phase model (DPM). A mathematical simulation model based on an enhanced DPM is built and a dynamic computing fluid mesh is generated. The dynamic model of IR smoke is then established using an extended equivalent-blackbody-molecule model. Experiments demonstrate that this model realizes a dynamic method for modeling IR smoke with higher veracity.

Tales of some ancient harbors in the Aegean back-arc region: Earthquakes, coastal changes, historical impacts

Science.gov (United States)

Stiros, Stathis; Saltogianni, Vasso

2017-04-01

Tectonically active terrains are characterized by seismic transient ground motions (shaking) and by permanent ground motions in the vicinity of activated faults, with both effects occasionally leaving their signature on human constructions and the landscape. Especially in coastal areas marked by small tidal ranges and normal water salinity, as is the case with most parts of the Eastern Mediterranean, even small-amplitude tectonic motions can be derived from observations on coastal constructions, mainly harbors, but also on spring chambers in nearly arid environments, sewers, etc. Such observations, if coupled with well-dated observations of destructions and repairs and of changes in the occupation style of ancient sites can permit precious information conveyed from archaeology to tectonics/seismology and vice versa. A transect with harbor remains from Rhodes to the Gulf of Corinth and then till the Ionian Islands provides some excellent examples. The military harbor of Rhodes, in an area of long term uplift, a coded report of which seems to be provided by ancient poet Pindar, was subject to seismic subsidence and destruction, but with major international support, it was repaired, till renewed uplift brought it several meters above the water. In the Corinth area, the Kenchreai harbor was abruptly submerged during a major repair of a temple, as revealed by precious stained glass panels, ready to use but abandoned in shallow water beneath ruins, while radiometric dating of the uplift in the western Lechaion harbor, constrains its excavation in swampy environment not in Roman times, but to the period of flourishing of Corinth in circa 600BC and the colonization of Italy. Farther west, the sea-level mark of the harbor of Aigeira, at Mavra Litharia (Derveni/Akrata) indicates 4m uplift since the Roman period, at least partly seismic, correlating with an exposed reef and the abandonment of the repairs of the theatre of Aigeira. Seismic land uplift explains the demise of

Side-scan imagery of Apra Harbor, Guam U.S. Territory

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Gridded side-scan imagery collected in Apra Harbor, Guam U.S. Territory, USA. The GeoTIFF and Arc ASCII file includes data that were collected using a Klein 3000...

Transgenic Mouse Model Harboring the Transcriptional Fusion Ccl20-Luciferase as a Novel Reporter of Pro-Inflammatory Response

Science.gov (United States)

Crispo, Martina; Van Maele, Laurye; Tabareau, Julien; Cayet, Delphine; Errea, Agustina; Ferreira, Ana María; Rumbo, Martin; Sirard, Jean Claude

2013-01-01

The chemokine CCL20, the unique ligand of CCR6 functions as an attractant of immune cells. Expression of CCL20 is induced by Toll-like Receptor (TLR) signaling or proinflammatory cytokine stimulation. However CCL20 is also constitutively produced at specific epithelial sites of mucosa. This expression profile is achieved by transcriptional regulation. In the present work we characterized regulatory features of mouse Ccl20 gene. Transcriptional fusions between the mouse Ccl20 promoter and the firefly luciferase (luc) encoding gene were constructed and assessed in in vitro and in vivo assays. We found that liver CCL20 expression and luciferase activity were upregulated by systemic administration of the TLR5 agonist flagellin. Using shRNA and dominant negative form specific for mouse TLR5, we showed that this expression was controlled by TLR5. To address in situ the regulation of gene activity, a transgenic mouse line harboring a functional Ccl20-luc fusion was generated. The luciferase expression was highly concordant with Ccl20 expression in different tissues. Our data indicate that the transgenic mouse model can be used to monitor activation of innate response in vivo. PMID:24265691

New world bats harbor diverse influenza A viruses.

Directory of Open Access Journals (Sweden)

Suxiang Tong

Full Text Available Aquatic birds harbor diverse influenza A viruses and are a major viral reservoir in nature. The recent discovery of influenza viruses of a new H17N10 subtype in Central American fruit bats suggests that other New World species may similarly carry divergent influenza viruses. Using consensus degenerate RT-PCR, we identified a novel influenza A virus, designated as H18N11, in a flat-faced fruit bat (Artibeus planirostris from Peru. Serologic studies with the recombinant H18 protein indicated that several Peruvian bat species were infected by this virus. Phylogenetic analyses demonstrate that, in some gene segments, New World bats harbor more influenza virus genetic diversity than all other mammalian and avian species combined, indicative of a long-standing host-virus association. Structural and functional analyses of the hemagglutinin and neuraminidase indicate that sialic acid is not a ligand for virus attachment nor a substrate for release, suggesting a unique mode of influenza A virus attachment and activation of membrane fusion for entry into host cells. Taken together, these findings indicate that bats constitute a potentially important and likely ancient reservoir for a diverse pool of influenza viruses.

33 CFR 117.181 - Oakland Inner Harbor Tidal Canal.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Oakland Inner Harbor Tidal Canal. 117.181 Section 117.181 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Tidal Canal. The draws of the Alameda County highway drawbridges at Park Street, mile 5.2; Fruitvale...

33 CFR 165.776 - Security Zone; Coast Guard Base San Juan, San Juan Harbor, Puerto Rico

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Security Zone; Coast Guard Base San Juan, San Juan Harbor, Puerto Rico 165.776 Section 165.776 Navigation and Navigable Waters COAST... Guard District § 165.776 Security Zone; Coast Guard Base San Juan, San Juan Harbor, Puerto Rico (a...

A Comparison of Microbial Water Quality and Diversity for Ballast and Tropical Harbor Waters.

Science.gov (United States)

Ng, Charmaine; Le, Thai-Hoang; Goh, Shin Giek; Liang, Liang; Kim, Yiseul; Rose, Joan B; Yew-Hoong, Karina Gin

2015-01-01

Indicator organisms and antibiotic resistance were used as a proxy to measure microbial water quality of ballast tanks of ships, and surface waters in a tropical harbor. The survival of marine bacteria in ballast tanks appeared to diminish over longer water retention time, with a reduction of cell viability observed after a week based on heterotrophic plate counts. Pyrosequencing of 16S rRNA genes showed distinct differences in microbial composition of ballast and harbor waters. The harbor waters had a higher abundance of operational taxonomic units (OTUs) assigned to Cyanobacteria (Synechococcus spp.) and α-proteobacteria (SAR11 members), while marine hydrocarbon degraders such as γ-proteobacteria (Ocenspirillaes spp., Thiotrchales spp.) and Bacteroidetes (Flavobacteriales spp.) dominated the ballast water samples. Screening of indicator organisms found Escherichia coli (E. coli), Enterococcus and Pseudomonas aeruginosa (P. aeruginosa) in two or more of the ballast and harbor water samples tested. Vibrio spp. and Salmonella spp. were detected exclusively in harbor water samples. Using quantitative PCR (qPCR), we screened for 13 antibiotic resistant gene (ARG) targets and found higher abundances of sul1 (4.13-3.44 x 102 copies/mL), dfrA (0.77-1.80 x10 copies/mL) and cfr (2.00-5.21 copies/mL) genes compared to the other ARG targets selected for this survey. These genes encode for resistance to sulfonamides, trimethoprim and chloramphenicol-florfenicol antibiotics, which are also known to persist in sediments of aquaculture farms and coastal environments. Among the ARGs screened, we found significant correlations (Pwater quality survey, quantitatively assessing indicators of antibiotic resistance, potentially pathogenic organisms and a broad-brush description of difference in microbial composition and diversity between open oceans and tropical coastal environments through the use of next generation sequencing technology.

Distribution and contamination status of chromium in surface sediments of northern Kaohsiung Harbor, Taiwan.

Science.gov (United States)

Dong, Cheng-Di; Chen, Chiu-Wen; Chen, Chih-Feng

2013-07-01

The distribution, enrichment, accumulation, and potential ecological risk of chromium (Cr) in the surface sediments of northern Kaohsiung Harbor, Taiwan, China were investigated. Sediment samples from ten locations located between the river mouths and harbor entrance of northern Kaohsiung Harbor were collected quarterly in 2011 and characterized for Cr, aluminum, water content, organic matter, total nitrogen, total phosphorous, total grease, and grain size. Results showed that the Cr concentrations varied from 27.0 to 361.9 mg/kg with an average of (113.5 +/- 87.0) mg/kg. High Cr concentration was observed near the Jen-Gen River mouth. The mean Cr concentration was high at 255.5 mg/kg, which was at least 2 to 7 times than that of other sites. This might imply significant Cr contribution from upstream receiving tanneries wastewater into the Jen-Gen River. The spatial distribution of Cr reveals relatively high in the river mouth region, especially in Jen-Gen River, and gradually diminishes toward the harbor entrance region. This indicates that the major sources of Cr pollution from upstream industrial and municipal wastewaters discharged along the river bank; and Cr may drift with sea current and be dispersed into open sea. Moreover, Cr concentrations correlated closely to the physical-chemical properties of the sediments, which suggested the influence of industrial and municipal wastewaters discharged from the neighboring industrial parks and river basins. Results from the enrichment factor and geo-accumulation index analyses imply that the Jen-Gen River sediments can be characterized as moderate enrichment and none to medium accumulation of Cr, respectively. However, results of potential ecological risk index indicate that the sediment has low ecological potential risk. The results can provide valuable information to developing future strategies for the management of river mouth and harbor.

Cultural Resources Survey of Mobile Harbor, Alabama.

Science.gov (United States)

1983-01-01

improvement from the point of view of supply and communication with other European settlements, since it cut the lightering distance to the capital in half...order to cut the costs of building (Bathe 1978:08.00-02; Millar 1978:15-29). 32 6e The sharing of ship builders, the borrowing of vessel lines and the... Eslava Street Mobile. Burned to water’s edge during overhaul. Notes: Served as HINGHAM in Boston Harbor; served as ORIENT in Long Island Sound. Operated

Organochlorines in harbor seal (Phoca vitulina) tissues from the northern Gulf of Alaska

International Nuclear Information System (INIS)

Wang Dongli; Atkinson, Shannon; Hoover-Miller, Anne; Lee, Sung-Eun; Li, Qing X.

2007-01-01

Polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethane (DDT) and its metabolites, heptachlor and hexachlorocyclohexanes (HCHs) were analyzed in the harbor seal (Phoca vitulina) tissues collected from the Gulf of Alaska during 2000-2001. ΣPCBs (16-728 ng/g lw) and ΣDDTs (14-368 ng/g lw) were the predominant pollutants followed by ΣHCHs (0.56-93 ng/g lw) and heptachlor (≤0.068-6.0 ng/g lw). Concentrations of the above organochlorines (OCs) in the liver, kidney and blubber tissues correlated with ages, sex, body weight and blubber thickness of the harbor seals. The OC concentrations were similar between the samples collected from two different regions - Prince William Sound, and Kodiak Island and Southern Alaska Peninsula. Mean levels of OCs in nursing female adults were much lower than those in male adults, which indicate that lactation transfer OCs from mother seals to newborns. - Σ28 PCB congeners, p,p'-DDE, p,p'-DDD and p,p'-DDT, α-, β-, γ-, and δ-HCH and heptachlor in harbor seal tissues from the northern Gulf of Alaska are reported

77 FR 60319 - Harbor Porpoise Take Reduction Plan; Coastal Gulf of Maine Closure Area Established With a...

Science.gov (United States)

2012-10-03

...-XC099 Harbor Porpoise Take Reduction Plan; Coastal Gulf of Maine Closure Area Established With a... Atmospheric Administration (NOAA), Commerce. ACTION: Establishment of the Coastal Gulf of Maine Closure Area... Service (NMFS) announces the establishment of the Coastal Gulf of Maine Closure Area under the Harbor...

77 FR 29929 - Safety Zone; Town of Cape Charles Fireworks, Cape Charles Harbor, Cape Charles, VA

Science.gov (United States)

2012-05-21

... section of this notice. Basis and Purpose On July 4, 2012 the Town of Cape Charles will sponsor a...-AA00 Safety Zone; Town of Cape Charles Fireworks, Cape Charles Harbor, Cape Charles, VA AGENCY: Coast... temporary safety zone on the waters of Cape Charles City Harbor in Cape Charles, VA in support of the Fourth...

Defense Infrastructure: General and Flag Officer Quarters at Pearl Harbor, Hawaii

National Research Council Canada - National Science Library

2002-01-01

.... We reviewed 17 GFOQs at Pearl Harbor, Hawaii, with budgeted maintenance and repair costs of $1,247,300, to determine whether the Navy had properly classified interior shutter costs as maintenance and repair...

Modeling cancer registration processes with an enhanced activity diagram.

Science.gov (United States)

Lyalin, D; Williams, W

2005-01-01

Adequate instruments are needed to reflect the complexity of routine cancer registry operations properly in a business model. The activity diagram is a key instrument of the Unified Modeling Language (UML) for the modeling of business processes. The authors aim to improve descriptions of processes in cancer registration, as well as in other public health domains, through the enhancements of an activity diagram notation within the standard semantics of UML. The authors introduced the practical approach to enhance a conventional UML activity diagram, complementing it with the following business process concepts: timeline, duration for individual activities, responsibilities for individual activities within swimlanes, and descriptive text. The authors used an enhanced activity diagram for modeling surveillance processes in the cancer registration domain. Specific example illustrates the use of an enhanced activity diagram to visualize a process of linking cancer registry records with external mortality files. Enhanced activity diagram allows for the addition of more business concepts to a single diagram and can improve descriptions of processes in cancer registration, as well as in other domains. Additional features of an enhanced activity diagram allow to advance the visualization of cancer registration processes. That, in turn, promotes the clarification of issues related to the process timeline, responsibilities for particular operations, and collaborations among process participants. Our first experiences in a cancer registry best practices development workshop setting support the usefulness of such an approach.

75 FR 43823 - Safety Zone; He'eia Kea Small Boat Harbor, Kaneohe Bay, Oahu, HI

Science.gov (United States)

2010-07-27

...The Coast Guard is establishing a temporary safety zone in He'eia Kea Small Boat Harbor located in Kaneohe Bay, Oahu, Hawaii. The safety zone is necessary to protect watercraft and the general public from hazards associated with five vessels moored for approximately 3- weeks off the boat harbor's main pier. Vessels desiring to transit through the zone can request permission by contacting the Captain of the Port Honolulu.

PAH- and PCB-induced Alterations of Protein Tyrosine Kinase and Cytokine Gene Transcription in Harbor Seal (Phoca Vitulina PBMC

Directory of Open Access Journals (Sweden)

Jennifer C. C. Neale

2005-01-01

Full Text Available Mechanisms underlying in vitro immunomodulatory effects of polycyclic aromatic hydrocarbons (PAHs and polychlorinated biphenyls (PCBs were investigated in harbor seal peripheral leukocytes, via real-time PCR. We examined the relative genetic expression of the protein tyrosine kinases (PTKs Fyn and Itk, which play a critical role in T cell activation, and IL-2, a cytokine of central importance in initiating adaptive immune responses. IL-1, the macrophage-derived pro-inflammatory cytokine of innate immunity, was also included as a measure of macrophage function. Harbor seal PBMC were exposed to the prototypic immunotoxic PAH benzo[a]pyrene (BaP, 3,3',4,4',5,5'-hexachlorobiphenyl (CB-169, a model immunotoxic PCB, or DMSO (vehicle control. Exposure of Con A-stimulated harbor seal PBMC to both BaP and CB-169 produced significantly altered expression in all four targets relative to vehicle controls. The PTKs Fyn and Itk were both up-regulated following exposure to BaP and CB-169. In contrast, transcripts for IL-2 and IL-1 were decreased relative to controls by both treatments. Our findings are consistent with those of previous researchers working with human and rodent systems and support a hypothesis of contaminant-altered lymphocyte function mediated (at least in part by disruption of T cell receptor (TCR signaling and cytokine production.

A 5' splice site enhances the recruitment of basal transcription initiation factors in vivo

DEFF Research Database (Denmark)

Damgaard, Christian Kroun; Kahns, Søren; Lykke-Andersen, Søren

2008-01-01

RNAs, harboring wild-type or various 5′ splice site mutations, we demonstrate a strong positive correlation between splicing efficiency and transcription activity. Interestingly, a 5′ splice site can stimulate transcription even in the absence of splicing. Chromatin immunoprecipitation experiments show enhanced...... a promoter-proximal 5′ splice site via its U1 snRNA interaction can feed back to stimulate transcription initiation by enhancing preinitiation complex assembly....

The Game Enhanced Learning Model

DEFF Research Database (Denmark)

Reng, Lars; Schoenau-Fog, Henrik

2016-01-01

will describe the levels of the model, which is based on our experience in teaching professional game development at university level. Furthermore, we have been using the model to inspire numerous educators to improve their students’ motivation and skills. The model presents various game-based learning...... activities, and depicts their required planning and expected outcome through eight levels. At its lower levels, the model contains the possibilities of using stand-alone analogue and digital games as teachers, utilizing games as a facilitator of learning activities, exploiting gamification and motivating......In this paper, we will introduce the Game Enhanced learning Model (GEM), which describes a range of gameoriented learning activities. The model is intended to give an overview of the possibilities of game-based learning in general and all the way up to purposive game productions. In the paper, we...

Aerial Survey Effort for Harbor Seals in Coastal Alaska (2004-2015)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — The most feasible approach to determining harbor seal distribution and abundance in Alaska coastal habitats is to use aircraft to count seals when they haul out of...

Selenium and mercury concentrations in harbor seals (Phoca vitulina) from central California: Health implications in an urbanized estuary

Science.gov (United States)

McHuron, Elizabeth A.; Harvey, James T.; Castellini, J. Margaret; Stricker, Craig A.; O'Hara, Todd M.

2014-01-01

We measured total selenium and total mercury concentrations ([TSe] and [THg]) in hair (n = 138) and blood (n = 73) of harbor seals (Phoca vitulina) from California to assess variation by geography and sex, and inferred feeding relationships based on carbon, nitrogen, and sulfur stable isotopes. Harbor seals from Hg-contaminated sites had significantly greater [THg], and lesser [TSe] and TSe:THg molar ratios than seals from a relatively uncontaminated site. Males had significantly greater [THg] than females at all locations. Sulfur stable isotope values explained approximately 25% of the variability in [THg], indicating increased Hg exposure for seals with a greater use of estuarine prey species. Decreased [TSe] in harbor seals from Hg-contaminated regions may indicate a relative Se deficiency to mitigate the toxic effects of Hg. Further investigation into the Se status and the potential negative impact of Hg on harbor seals from Hg-contaminated sites is warranted.

Emergence of fatal avian influenza in New England harbor seals

Science.gov (United States)

Anthony, S.J.; St. Leger, J. A.; Pugliares, K.; Ip, Hon S.; Chan, J.M.; Carpenter, Z.W.; Navarrete-Macias, I.; Sanchez-Leon, M.; Saliki, J.T.; Pedersen, J.; Karesh, W.; Daszak, P.; Rabadan, R.; Rowles, T.; Lipkin, W.I.

2012-01-01

From September to December 2011, 162 New England harbor seals died in an outbreak of pneumonia. Sequence analysis of postmortem samples revealed the presence of an avian H3N8 influenza A virus, similar to a virus circulating in North American waterfowl since at least 2002 but with mutations that indicate recent adaption to mammalian hosts. These include a D701N mutation in the viral PB2 protein, previously reported in highly pathogenic H5N1 avian influenza viruses infecting people. Lectin staining and agglutination assays indicated the presence of the avian-preferred SAα-2,3 and mammalian SAα-2,6 receptors in seal respiratory tract, and the ability of the virus to agglutinate erythrocytes bearing either the SAα-2,3 or the SAα-2,6 receptor. The emergence of this A/harbor seal/Massachusetts/1/2011 virus may herald the appearance of an H3N8 influenza clade with potential for persistence and cross-species transmission.

77 FR 47331 - Regulated Navigation Area-New Haven Harbor, Quinnipiac River, Mill River, New Haven, CT; Pearl...

Science.gov (United States)

2012-08-08

...-AA11 Regulated Navigation Area--New Haven Harbor, Quinnipiac River, Mill River, New Haven, CT; Pearl... navigable waters of New Haven Harbor, Quinnipiac River and Mill River. The current RNA pertains only to the..., Quinnipiac River, and Mill River RNA. The proposed amendment would give the Captain of the Port Sector Long...

78 FR 7663 - SLR; 2013 International Rolex Regatta; St. Thomas Harbor; St. Thomas, U.S. Virgin Islands

Science.gov (United States)

2013-02-04

... 1625-AA08 SLR; 2013 International Rolex Regatta; St. Thomas Harbor; St. Thomas, U.S. Virgin Islands... special local regulations on the waters of St. Thomas Harbor in St. Thomas, U. S. Virgin Islands during... 5 p.m., Monday through Friday, except federal holidays. The telephone number is 202-366-9329. See...

Surveillance for zoonotic and selected pathogens in harbor seals Phoca vitulina from central California

Science.gov (United States)

Greig, Denise J.; Ip, Hon S.; Gulland, Frances M. D.; Miller, Woutrina A.; Conrad, Patricia A.; Field, Cara L.; Fleetwood, Michelle; Harvey, James T.; Jang, Spencer; Packham, Andrea; Wheeler, Elizabeth; Hall, Ailsa J.

2014-01-01

The infection status of harbor seals Phoca vitulina in central California, USA, was evaluated through broad surveillance for pathogens in stranded and wild-caught animals from 2001 to 2008, with most samples collected in 2007 and 2008. Stranded animals from Mendocino County to San Luis Obispo County were sampled at a rehabilitation facility: The Marine Mammal Center (TMMC, n = 175); wild-caught animals were sampled at 2 locations: San Francisco Bay (SF, n = 78) and Tomales Bay (TB, n = 97), that differed in degree of urbanization. Low prevalences of Salmonella, Campylobacter, Giardia, and Cryptosporidium were detected in the feces of stranded and wild-caught seals. Clostridium perfringens and Escherichia coli were more prevalent in the feces of stranded (58% [78 out of 135] and 76% [102 out of 135]) than wild-caught (42% [45 out of 106] and 66% [68 out of 106]) seals, whereas Vibrio spp. were 16 times more likely to be cultured from the feces of seals from SF than TB or TMMC (p neurona, and type A influenza was only detected in the wild-caught harbor seals (post-weaning age classes), whereas antibody titers to Leptospira spp. were detected in stranded and wild-caught seals. No stranded (n = 109) or wild-caught (n = 217) harbor seals had antibodies to phocine distemper virus, although a single low titer to canine distemper virus was detected. These results highlight the role of harbor seals as sentinel species for zoonotic and terrestrial pathogens in the marine environment.

Retail ready-to-eat food as a potential vehicle for Staphylococcus spp. harboring antibiotic resistance genes.

Science.gov (United States)

Chajęcka-Wierzchowska, Wioleta; Zadernowska, Anna; Nalepa, Beata; Sierpińska, Magda; Laniewska-Trokenheim, Lucja

2014-06-01

Ready-to-eat (RTE) food, which does not need thermal processing before consumption, could be a vehicle for the spread of antibiotic-resistant microorganisms. As part of general microbiological safety checks, staphylococci are routinely enumerated in these kinds of foods. However, the presence of antibiotic-resistant staphylococci in RTE food is not routinely investigated, and data are only available from a small number of studies. The present study evaluated the pheno- and genotypical antimicrobial resistance profile of Staphylococcus spp. isolated from 858 RTE foods (cheeses, cured meats, sausages, smoked fishes, salads). Of 113 strains isolated, S. aureus was the most prevalent species, followed by S. xylosus, S. saprophyticus, and S. epidermidis. More than half (54.9%) of the isolates were resistant to at least one class of tested antibiotic; of these, 35.4% of the strains were classified as multidrug resistant. Most of the isolates were resistant to cefoxitin (49.6%), followed by clindamycin (39.3%), tigecycline (27.4%), quinupristin-dalfopristin (22.2%), rifampin (20.5%), tetracycline (17.9%), and erythromycin (8.5%). All methicillin-resistant staphylococci harbored the mecA gene. Among the isolates resistant to at least one antibiotic, 38 harbored tetracycline resistance determinant tet (M), 24 harbored tet (L), and 9 harbored tet (K). Of the isolates positive for tet (M) genes, 34.2% were positive for the Tn916-Tn1545-like integrase family gene. Our results indicated that retail RTE food could be considered an important route for the transmission of antibiotic-resistant bacteria harboring multiple antibiotic resistance genes.

An investigation of the feasibility of building a harbor on the West coast of South America using explosive power of nuclear weapons, a preliminary report

Energy Technology Data Exchange (ETDEWEB)

Zodtner, H. H.

1971-12-31

There is an interest in discovering the various peace time uses of nuclear explosives. One of the proposals is the building of harbors. There are several ports along the west coast of South America where lighterage is necessary. This implies a need for expanded harbor facilities. The problem is to find a good location for creating a harbor, and the feasibility of accomplishing this with the use of nuclear force. Feasibility includes blast effects, radiation hazards, the number of weapons needed, and economic considerations. Economic considerations include the cost of treating a harbor of sufficient depth and area, the building of harbor facilities, and the estimated savings and advantages of the new harbor. Several meetings were held with naval personnel of the Military Liaison group at UCRL to discuss the general problems of harbors. Thirty-three different ports were given a preliminary investigation.

78 FR 63381 - Safety Zones; Hawaiian Island Commercial Harbors, HI

Science.gov (United States)

2013-10-24

...; Kaumalapau, Lanai; Kahului, Maui and Kawaihae and Hilo on the Island of Hawaii). The purpose of these safety... the piers faces. (9) All waters of Hilo Harbor, Hawaii immediately adjacent to commercial piers 1 and.... SUMMARY: The Coast Guard is establishing nine (9) permanent safety zones encompassing Hawaii's commercial...

33 CFR 110.197 - Galveston Harbor, Bolivar Roads Channel, Texas.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Galveston Harbor, Bolivar Roads Channel, Texas. 110.197 Section 110.197 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Roads Channel, Texas. (a)(1) Anchorage area (A). The water bounded by a line connecting the following...

A Comparison of Microbial Water Quality and Diversity for Ballast and Tropical Harbor Waters.

Directory of Open Access Journals (Sweden)

Charmaine Ng

Full Text Available Indicator organisms and antibiotic resistance were used as a proxy to measure microbial water quality of ballast tanks of ships, and surface waters in a tropical harbor. The survival of marine bacteria in ballast tanks appeared to diminish over longer water retention time, with a reduction of cell viability observed after a week based on heterotrophic plate counts. Pyrosequencing of 16S rRNA genes showed distinct differences in microbial composition of ballast and harbor waters. The harbor waters had a higher abundance of operational taxonomic units (OTUs assigned to Cyanobacteria (Synechococcus spp. and α-proteobacteria (SAR11 members, while marine hydrocarbon degraders such as γ-proteobacteria (Ocenspirillaes spp., Thiotrchales spp. and Bacteroidetes (Flavobacteriales spp. dominated the ballast water samples. Screening of indicator organisms found Escherichia coli (E. coli, Enterococcus and Pseudomonas aeruginosa (P. aeruginosa in two or more of the ballast and harbor water samples tested. Vibrio spp. and Salmonella spp. were detected exclusively in harbor water samples. Using quantitative PCR (qPCR, we screened for 13 antibiotic resistant gene (ARG targets and found higher abundances of sul1 (4.13-3.44 x 102 copies/mL, dfrA (0.77-1.80 x10 copies/mL and cfr (2.00-5.21 copies/mL genes compared to the other ARG targets selected for this survey. These genes encode for resistance to sulfonamides, trimethoprim and chloramphenicol-florfenicol antibiotics, which are also known to persist in sediments of aquaculture farms and coastal environments. Among the ARGs screened, we found significant correlations (P<0.05 between ereA, ermG, cfr and tetO genes to one or more of the indicator organisms detected in this study, which may suggest that these members contribute to the environmental resistome. This study provides a baseline water quality survey, quantitatively assessing indicators of antibiotic resistance, potentially pathogenic organisms and a

Vector-Interaction-Enhanced Bag Model

Science.gov (United States)

Cierniak, Mateusz; Klähn, Thomas; Fischer, Tobias; Bastian, Niels-Uwe

2018-02-01

A commonly applied quark matter model in astrophysics is the thermodynamic bag model (tdBAG). The original MIT bag model approximates the effect of quark confinement, but does not explicitly account for the breaking of chiral symmetry, an important property of Quantum Chromodynamics (QCD). It further ignores vector repulsion. The vector-interaction-enhanced bag model (vBag) improves the tdBAG approach by accounting for both dynamical chiral symmetry breaking and repulsive vector interactions. The latter is of particular importance to studies of dense matter in beta-equilibriumto explain the two solar mass maximum mass constraint for neutron stars. The model is motivated by analyses of QCD based Dyson-Schwinger equations (DSE), assuming a simple quark-quark contact interaction. Here, we focus on the study of hybrid neutron star properties resulting from the application of vBag and will discuss possible extensions.

A neurological comparative study of the harp seal (Pagophilus groenlandicus) and harbor porpoise (Phocoena phocoena) brain

DEFF Research Database (Denmark)

Walløe, Solveig; Eriksen, Nina; Dabelsteen, Torben

2010-01-01

The cetacean brain is well studied. However, few comparisons have been done with other marine mammals. In this study, we compared the harp seal (Pagophilus groenlandicus) and the harbor porpoise brain (Phocoena phocoena). Stereological methods were applied to compare three areas of interest...... cells, whereas the harp seal have 6.1 × 10(9) neocortical neurons and 17.5 × 10(9) neocortical glial cells. The harbor porpoise have significantly more neurons and glial cells in the auditory cortex than in the visual cortex, whereas the pattern was opposite for the harp seal. These results...... are the first to provide estimates of the number of neurons and glial cells in the neocortex of the harp seal and harbor porpoise brain and offer new data to the comparative field of mammalian brain evolution....

ENHANCING STUDENTS’ COMMUNICATION SKILLS THROUGH TREFFINGER TEACHING MODEL

Directory of Open Access Journals (Sweden)

Idrus Alhaddad

2015-01-01

Full Text Available This research aims to  investigate, compare, and describe the achievement and enhancement of students’ mathematical communication skills (MCS.  It based on the prior mathematical knowledge (PMK category (high, medium and low by using Treffinger models (TM and conventional learning (CL. This research is an experimental study with the population of all students of Mathematics Education Department who took Discrete Mathematics subject matter of one university in the city of Ternate. The results show that (1 the achievement and enhancement of MCS  students that used  TM are higher than the students learning using CL; (2 Based on the categories of PMK, the achievement and enhancement of MCS of students using TM are also higher than those learning with CL; and  (3 There was no interaction effect between learning (TM and CL and PMK to the achievement and enhancement of MCS of the students.Keyword: Communication Skills, Prior Mathematical Knowledge, Treffinger Model DOI: dx.doi.org/10.22342/jme.61.31

Therapeutic strategies to overcome crizotinib resistance in non-small cell lung cancers harboring the fusion oncogene EML4-ALK

Science.gov (United States)

Katayama, Ryohei; Khan, Tahsin M.; Benes, Cyril; Lifshits, Eugene; Ebi, Hiromichi; Rivera, Victor M.; Shakespeare, William C.; Iafrate, A. John; Engelman, Jeffrey A.; Shaw, Alice T.

2011-01-01

The echinoderm microtubule-associated protein-like 4 (EML4)-anaplastic lymphoma kinase (ALK) fusion oncogene represents a molecular target in a small subset of non-small cell lung cancers (NSCLCs). This fusion leads to constitutive ALK activation with potent transforming activity. In a pivotal phase 1 clinical trial, the ALK tyrosine kinase inhibitor (TKI) crizotinib (PF-02341066) demonstrated impressive antitumor activity in the majority of patients with NSCLC harboring ALK fusions. However, despite these remarkable initial responses, cancers eventually develop resistance to crizotinib, usually within 1 y, thereby limiting the potential clinical benefit. To determine how cancers acquire resistance to ALK inhibitors, we established a model of acquired resistance to crizotinib by exposing a highly sensitive EML4-ALK–positive NSCLC cell line to increasing doses of crizotinib until resistance emerged. We found that cells resistant to intermediate doses of crizotinib developed amplification of the EML4-ALK gene. Cells resistant to higher doses (1 μM) also developed a gatekeeper mutation, L1196M, within the kinase domain, rendering EML4-ALK insensitive to crizotinib. This gatekeeper mutation was readily detected using a unique and highly sensitive allele-specific PCR assay. Although crizotinib was ineffectual against EML4-ALK harboring the gatekeeper mutation, we observed that two structurally different ALK inhibitors, NVP-TAE684 and AP26113, were highly active against the resistant cancer cells in vitro and in vivo. Furthermore, these resistant cells remained highly sensitive to the Hsp90 inhibitor 17-AAG. Thus, we have developed a model of acquired resistance to ALK inhibitors and have shown that second-generation ALK TKIs or Hsp90 inhibitors are effective in treating crizotinib-resistant tumors harboring secondary gatekeeper mutations. PMID:21502504

Rhizosphere of rice plants harbor bacteria with multiple plant growth ...

African Journals Online (AJOL)

Rhizosphere of rice plants harbor bacteria with multiple plant growth promoting features. ... 45 (39.46%) isolates were capable of producing siderophore, the range of production being 4.50 to 223.26 μg mg-1 protein. Analysis of molecular diversity was made by amplified ribosomal DNA restriction analysis (ARDRA) and ...

33 CFR 117.669 - St. Louis River (Duluth Superior Harbor).

Science.gov (United States)

2010-07-01

... SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Minnesota § 117.669 St. Louis River (Duluth Superior Harbor). (a) The draws of the Burlington Northern railroad bridge, mile 5.7, shall open... Missabe and Iron Range Railway bridge, mile 16.3, need not be opened for the passage of vessels. The owner...

33 CFR 117.1083 - Duluth-Superior Harbor (St. Louis River).

Science.gov (United States)

2010-07-01

... SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Wisconsin § 117.1083 Duluth-Superior Harbor (St. Louis River). (a) The draws of the Burlington Northern railroad bridge, mile 5.7 at... blasts. (c) The draw of the Duluth, Missabe and Iron Range Railway bridge, mile 16.3 at Duluth, need not...

Legacy model integration for enhancing hydrologic interdisciplinary research

Science.gov (United States)

Dozier, A.; Arabi, M.; David, O.

2013-12-01

Many challenges are introduced to interdisciplinary research in and around the hydrologic science community due to advances in computing technology and modeling capabilities in different programming languages, across different platforms and frameworks by researchers in a variety of fields with a variety of experience in computer programming. Many new hydrologic models as well as optimization, parameter estimation, and uncertainty characterization techniques are developed in scripting languages such as Matlab, R, Python, or in newer languages such as Java and the .Net languages, whereas many legacy models have been written in FORTRAN and C, which complicates inter-model communication for two-way feedbacks. However, most hydrologic researchers and industry personnel have little knowledge of the computing technologies that are available to address the model integration process. Therefore, the goal of this study is to address these new challenges by utilizing a novel approach based on a publish-subscribe-type system to enhance modeling capabilities of legacy socio-economic, hydrologic, and ecologic software. Enhancements include massive parallelization of executions and access to legacy model variables at any point during the simulation process by another program without having to compile all the models together into an inseparable 'super-model'. Thus, this study provides two-way feedback mechanisms between multiple different process models that can be written in various programming languages and can run on different machines and operating systems. Additionally, a level of abstraction is given to the model integration process that allows researchers and other technical personnel to perform more detailed and interactive modeling, visualization, optimization, calibration, and uncertainty analysis without requiring deep understanding of inter-process communication. To be compatible, a program must be written in a programming language with bindings to a common

Restoration Science in New York Harbor: It takes a (large, diverse and engaged) village

Science.gov (United States)

Newton, R.; Birney, L.; Janis, S.; Groome, M.; Palmer, M.; Bone, E.; O'Neil, J. M.; Hill, J.; Dennison, W.; Malinowski, P.; Kohne, L.; Molina, M.; Moore, G.; Woods, N.

2015-12-01

The Curriculum + Community Enterprise for Restoration Science (CCE-RS) facilitates partnerships between scientists and middle school educators on ecological restoration and environmental monitoring projects. The educational model is designed to wrap around the student, including classroom instruction, field science, after-school programs and engagement with the student's community. Its pillars include: a teacher training fellowship at Pace University, student curriculum, a digital platform, afterschool and summer mentoring, and community exhibits. The digital platform includes a tablet app tailored to the project's field protocols and linked to a database shared across schools and partnering institutions. Through the digital platform, data is integrated into a single citizen-science monitoring project, teachers share curriculum and best practices, and students link directly to their peers at other schools. Curriculum development has been collaborative between scientists, science education specialists, and secondary school teachers. The CCE-RS is rooted in project-based learning: the New York Harbor School has engaged high school students in environmental monitoring and oyster restoration in the Harbor for about the last decade. The science partners (U. of Maryland and Columbia) have been working with students and other citizen scientists in outdoor science over about the last decade. Local partners in outside-the-classroom education include the New York Academy of Sciences, The River Project, which will provide field education services, and Good Shepherd Services, which provides after-school programming in schools serving primarily poor families. Scientists on the project engage directly with teachers and informal educators in curriculum development and citizen-science outreach. We present the lessons learned from our first cohort of Fellows, the pedagogical model, and the digital platform, which is extensible to other ecological restoration settings.

Re-alimentation in harbor seal pups: effects on the somatotropic axis and growth rate.

Science.gov (United States)

Richmond, Julie P; Norris, Tenaya; Zinn, Steven A

2010-01-15

The metabolic hormones, growth hormone (GH) and insulin-like growth factor (IGF)-I, together with IGF binding proteins (IGFBP), have been well studied in domestic species and are the primary components of the somatotropic axis. This hormone axis is responsive to nutrient intake, associated with growth rate, and accretion of protein and adipose. However, this relationship has not been evaluated in species that rely heavily on adipose stores for survival, such as pinnipeds. The primary objectives of this research were to investigate the response of the somatotropic axis to reduced nutrient intake and re-alimentation in rehabilitated harbor seal pups, and to assess if these hormones are related to nutritional status and growth rate in harbor seals. Stranded harbor seal pups (n=24) arrived at the rehabilitation facility very thin after fasting for several days (nutritional nadir). Throughout rehabilitation nutrient intake increased and pups gained mass and body condition. Concentrations of GH and IGFBP-2 decreased with re-alimentation, while IGF-I and IGFBP-3 concentrations increased. Overall, GH and IGFBP-2 were negatively associated and IGF-I and IGFBP-3 were positively associated with growth rate and increased body condition of harbor sea pups. Further, the magnitude of the growth response was related to the magnitude in response of the somatotropic axis to varied levels of intake. These data suggest that multiple components of the somatotropic axis may be used to assess the energy status of individuals and may also provide information on the level of feed intake that is predictive of growth rate.

Japan's Monroe Doctrine?: Re-Framing the Story of Pearl Harbor

Science.gov (United States)

Murnane, John R.

2007-01-01

Ever since War Department propaganda films set the mold, historians have pretty much followed: the road to Pearl Harbor began in 1931 with Japanese aggression in Manchuria. Any "revision" regarding the War in the Pacific has its own set of problems--engendering controversy, often running up against patriotic sentiments and the tendency…

Genes of the unfolded protein response pathway harbor risk alleles for primary open angle glaucoma.

Directory of Open Access Journals (Sweden)

Mary Anna Carbone

Full Text Available The statistical power of genome-wide association (GWA studies to detect risk alleles for human diseases is limited by the unfavorable ratio of SNPs to study subjects. This multiple testing problem can be surmounted with very large population sizes when common alleles of large effects give rise to disease status. However, GWA approaches fall short when many rare alleles may give rise to a common disease, or when the number of subjects that can be recruited is limited. Here, we demonstrate that this multiple testing problem can be overcome by a comparative genomics approach in which an initial genome-wide screen in a genetically amenable model organism is used to identify human orthologues that may harbor risk alleles for adult-onset primary open angle glaucoma (POAG. Glaucoma is a major cause of blindness, which affects over 60 million people worldwide. Several genes have been associated with juvenile onset glaucoma, but genetic factors that predispose to adult onset primary open angle glaucoma (POAG remain largely unknown. Previous genome-wide analysis in a Drosophila ocular hypertension model identified transcripts with altered regulation and showed induction of the unfolded protein response (UPR upon overexpression of transgenic human glaucoma-associated myocilin (MYOC. We selected 16 orthologous genes with 62 polymorphic markers and identified in two independent human populations two genes of the UPR that harbor POAG risk alleles, BIRC6 and PDIA5. Thus, effectiveness of the UPR in response to accumulation of misfolded or aggregated proteins may contribute to the pathogenesis of POAG and provide targets for early therapeutic intervention.

Genes of the unfolded protein response pathway harbor risk alleles for primary open angle glaucoma.

Science.gov (United States)

Carbone, Mary Anna; Chen, Yuhong; Hughes, Guy A; Weinreb, Robert N; Zabriskie, Norman A; Zhang, Kang; Anholt, Robert R H

2011-01-01

The statistical power of genome-wide association (GWA) studies to detect risk alleles for human diseases is limited by the unfavorable ratio of SNPs to study subjects. This multiple testing problem can be surmounted with very large population sizes when common alleles of large effects give rise to disease status. However, GWA approaches fall short when many rare alleles may give rise to a common disease, or when the number of subjects that can be recruited is limited. Here, we demonstrate that this multiple testing problem can be overcome by a comparative genomics approach in which an initial genome-wide screen in a genetically amenable model organism is used to identify human orthologues that may harbor risk alleles for adult-onset primary open angle glaucoma (POAG). Glaucoma is a major cause of blindness, which affects over 60 million people worldwide. Several genes have been associated with juvenile onset glaucoma, but genetic factors that predispose to adult onset primary open angle glaucoma (POAG) remain largely unknown. Previous genome-wide analysis in a Drosophila ocular hypertension model identified transcripts with altered regulation and showed induction of the unfolded protein response (UPR) upon overexpression of transgenic human glaucoma-associated myocilin (MYOC). We selected 16 orthologous genes with 62 polymorphic markers and identified in two independent human populations two genes of the UPR that harbor POAG risk alleles, BIRC6 and PDIA5. Thus, effectiveness of the UPR in response to accumulation of misfolded or aggregated proteins may contribute to the pathogenesis of POAG and provide targets for early therapeutic intervention.

Seroprevalence of Brucella antibodies in harbor seals in Alaska, USA, with age, regional, and reproductive comparisons.

Science.gov (United States)

Hoover-Miller, A; Dunn, J L; Field, C L; Blundell, G; Atkinson, S

2017-09-20

Populations of harbor seal Phoca vitulina in the Gulf of Alaska have dramatically declined during the past 4 decades. Numbers of seals in Glacier Bay, in southeast Alaska, USA, have also declined despite extensive protection. Causes of the declines and slow recovery are poorly understood. Brucellosis is a zoonotic disease that adversely affects reproduction in many domestic species. We measured the seroprevalence of Brucella antibodies in 554 harbor seals in 3 Alaska locations: Prince William Sound (PWS), Glacier Bay (GB), and Tracy Arm Fords Terror (TAFT) Wilderness Area. Objectives included testing for regional, sex, age, and female reproductive state differences in Brucella antibody seroprevalence, persistence in titers in recaptured seals, and differences in titers between mother seals and their pups. Overall, 52% of adults (AD), 53% of subadults (SA), 77% of yearlings (YRL), and 26% of Brucella. Results show higher seroprevalence (64%) for AD and SA seals in the depressed and declining populations in PWS and GB than in TAFT (29%). Lactating females were less likely to be seropositive than other AD females, including pregnant females. Further research is needed to seek evidence of Brucella infection in Alaskan harbor seals, identify effects on neonatal viability, and assess zoonotic implications for Alaska Natives who rely on harbor seals for food.

Enhanced Publications: Data Models and Information Systems

Directory of Open Access Journals (Sweden)

Alessia Bardi

2014-04-01

Full Text Available “Enhanced publications” are commonly intended as digital publications that consist of a mandatory narrative part (the description of the research conducted plus related “parts”, such as datasets, other publications, images, tables, workflows, devices. The state-of-the-art on information systems for enhanced publications has today reached the point where some kind of common understanding is required, in order to provide the methodology and language for scientists to compare, analyse, or simply discuss the multitude of solutions in the field. In this paper, we thoroughly examined the literature with a two-fold aim: firstly, introducing the terminology required to describe and compare structural and semantic features of existing enhanced publication data models; secondly, proposing a classification of enhanced publication information systems based on their main functional goals.

78 FR 16211 - Safety Zone, Corp. Event Finale UHC, St. Thomas Harbor; St. Thomas, U.S. Virgin Islands

Science.gov (United States)

2013-03-14

... 1625-AA00 Safety Zone, Corp. Event Finale UHC, St. Thomas Harbor; St. Thomas, U.S. Virgin Islands... establish a temporary safety zone on the waters of St. Thomas Harbor in St. Thomas, U.S. Virgin Islands... through Friday, except federal holidays. The telephone number is 202-366-9329. See the ``Public...

78 FR 23489 - Safety Zone; V.I. Carnival Finale, St. Thomas Harbor; St. Thomas, U.S.V.I.

Science.gov (United States)

2013-04-19

... waters of St. Thomas Harbor in St. Thomas, U.S. Virgin Islands during the V.I. Carnival Finale, a... through Friday, except Federal holidays. FOR FURTHER INFORMATION CONTACT: If you have questions on this... event. The event will be held on the waters of St. Thomas Harbor, St. Thomas, U. S. Virgin Islands...

Influence of the Pearl River estuary and vertical mixing in Victoria Harbor on water quality in relation to eutrophication impacts in Hong Kong waters.

Science.gov (United States)

Yin, Kedong; Harrison, Paul J

2007-06-01

This study presents water quality parameters such as nutrients, phytoplankton biomass and dissolved oxygen based on 11 years of water quality data in Victoria Harbor and examined how the Pearl River estuary discharge in summer and year round sewage discharge influenced these parameters. Nutrients in Victoria Harbor were strongly influenced by both the Pearl River and sewage effluent, as indicated by the high NO(3) inputs from the Pearl River in summer and higher NH(4) and PO(4) in Victoria Harbor than both its sides. N:P ratios were low in the dry season, but increased to >16:1 in the wet season, suggesting that P is potentially the most limiting nutrient in this area during the critical period in the summer. Although there were generally high nutrients, the phytoplankton biomass was not as high as one would expect in Victoria Harbor. In fact, there were high concentrations of chl near the bottom well below the photic zone. Salinity near the bottom was lower in Victoria Harbor than at the two entrances to Victoria Harbor, suggesting strong vertical mixing within Victoria Harbor. Therefore, strong vertical mixing and horizontal advection appear to play an important role in significantly reducing eutrophication impacts in Victoria Harbor. Consequently, dissolved oxygen near the bottom was low in summer, but only occasionally dipped to 2 mgL(-1) despite the high organic loading from sewage effluent.

A Technology Enhanced Learning Model for Quality Education

Science.gov (United States)

Sherly, Elizabeth; Uddin, Md. Meraj

Technology Enhanced Learning and Teaching (TELT) Model provides learning through collaborations and interactions with a framework for content development and collaborative knowledge sharing system as a supplementary for learning to improve the quality of education system. TELT deals with a unique pedagogy model for Technology Enhanced Learning System which includes course management system, digital library, multimedia enriched contents and video lectures, open content management system and collaboration and knowledge sharing systems. Open sources like Moodle and Wiki for content development, video on demand solution with a low cost mid range system, an exhaustive digital library are provided in a portal system. The paper depicts a case study of e-learning initiatives with TELT model at IIITM-K and how effectively implemented.

78 FR 19158 - Safety Zone; USA Triathlon, Milwaukee Harbor, Milwaukee, WI

Science.gov (United States)

2013-03-29

... have minimal impact on the economy, will not interfere with other agencies, will not adversely alter... Milwaukee Harbor due to the 2013 and 2014 Olympic and Sprint Distance National Championships. This proposed... the 2013 and 2014 Olympic and Sprint Distance National Championships. DATES: Comments and related...

Sedimentation and chronology of heavy metal pollution in Oslo harbor, Norway

DEFF Research Database (Denmark)

Lepland, Aivo; Andersen, Thorbjørn Joest; Lepland, Aave

2010-01-01

Stratigraphic profiles of Cu, Cd and Hg in ten sediment cores from the Oslo harbor, Norway, combined with results of radiometric dating demonstrate that pollution by these metals peaked between 1940 and 1970. Dating results indicate that Hg discharges peaked between 1940 and 1950, Cd reached...

Enhanced democratic learning within the Aalborg Model

DEFF Research Database (Denmark)

Qvist, Palle

2010-01-01

The Aalborg PBL Model [Kjersdam & Enemark, 1997; Kolmos et al., 2004] is an example of a democratic learning system [Qvist, 2008]. Writing one project each semester in teams is an important element in the model. Medicine with Industrial Specialisation - a study at the Faculties of Engineering......, Science and Medicine at Aalborg University - has combined the Aalborg Model with solving cases as used by other models. A questionnaire survey related to democratic learning indicates that the democratic learning has been enhanced. This paper presents the results....

Nonindigenous Marine Species Introductions in the harbors of the South and West Shores of Oahu, Hawaii 1997-1998 (NODC Accession 0000324)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Despite the potential importance of Honolulu Harbor or other commercial harbors on Oahu as potential gateways for nonindigenous marine species to enter the Hawaiian...

Introduced ascidians harbor highly diverse and host-specific symbiotic microbial assemblages.

Science.gov (United States)

Evans, James S; Erwin, Patrick M; Shenkar, Noa; López-Legentil, Susanna

2017-09-08

Many ascidian species have experienced worldwide introductions, exhibiting remarkable success in crossing geographic borders and adapting to local environmental conditions. To investigate the potential role of microbial symbionts in these introductions, we examined the microbial communities of three ascidian species common in North Carolina harbors. Replicate samples of the globally introduced species Distaplia bermudensis, Polyandrocarpa anguinea, and P. zorritensis (n = 5), and ambient seawater (n = 4), were collected in Wrightsville Beach, NC. Microbial communities were characterized by next-generation (Illumina) sequencing of partial (V4) 16S rRNA gene sequences. Ascidians hosted diverse symbiont communities, consisting of 5,696 unique microbial OTUs (at 97% sequenced identity) from 47 bacterial and three archaeal phyla. Permutational multivariate analyses of variance revealed clear differentiation of ascidian symbionts compared to seawater bacterioplankton, and distinct microbial communities inhabiting each ascidian species. 103 universal core OTUs (present in all ascidian replicates) were identified, including taxa previously described in marine invertebrate microbiomes with possible links to ammonia-oxidization, denitrification, pathogenesis, and heavy-metal processing. These results suggest ascidian microbial symbionts exhibit a high degree of host-specificity, forming intimate associations that may contribute to host adaptation to new environments via expanded tolerance thresholds and enhanced holobiont function.

Factors Contributing to Corrosion of Steel Pilings in Duluth-Superior Harbor

Science.gov (United States)

2009-11-01

1226 Office of Counsel,Code 1008.3 ADOR/Director NCST E. R. Franchi , 7000 Public Affairs (Unclassified/ Unlimited Only), Code 703o 4...Great Lakes. Accelerated corrosion of CS pilings in estua- rine and marine harbors is a global phenomenon.9 The term "accelerated low water corrosion

33 CFR 334.1420 - Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing...

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Pacific Ocean off Orote Point, Apra Harbor, Island of Guam, Marianas Islands; small arms firing range. 334.1420 Section 334.1420... AND RESTRICTED AREA REGULATIONS § 334.1420 Pacific Ocean off Orote Point, Apra Harbor, Island of Guam...

Assessment of the impact of oil spill on Mumbai harbor bay

International Nuclear Information System (INIS)

Pandit, G.G.; Saradhi, I.V.; Puranik, V.D.; Sharma, D.N.; Ghosh, A.K.

2011-04-01

On 7 th August, 2010 two cargo ships MSC Chitra and MV Khalijia collided off Mumbai coast causing an oil spill that spread quickly through Maharashtra's coastline. MSC Chitra ruptured its tank when it hit incoming MV Khalijia. MSC Chitra tilted to about 80 degrees soon after the collision, spilling an estimated 400 to 500 tonnes of oil. The ship was loaded with an estimated 2,600 tonnes of oil, 300 tonnes of diesel and 70 tonnes of lubricating oil at the time of the accident. The oil spill has spreaded over an area of 25 square kilometers. MSC Chitra was carrying 1219 containers out of which 31 had hazardous chemicals like organophosphate pesticides. After the collision about 400 containers fall off into to the sea from the deck of MSC Chitra. The oil spill has resulted in severe environmental damage along the Mumbai coastline as well as to the marine life. In order to assess the impact of oil spill on the marine environment daily monitoring of oil and grease levels has been carried out at CIRUS Jetty. Additionally three field surveys have been carried out along the coast line of Mumbai harbor bay from Vashi Jetty to Uran collecting sea water samples. The samples collected at CIRUS Jetty and Mumbai harbor bay are analyzed for various water quality parameters apart from oil and grease. The results indicate the oil and grease levels in sea water at CIRUS Jetty reduced to background levels within 15 days. The filed survey along Mumbai harbor bay also shows a decreasing trend in the oil and grease levels in seawater. (author)

78 FR 16208 - Safety Zone; V. I. Carnival Finale; St. Thomas Harbor; St. Thomas, U.S. Virgin Islands

Science.gov (United States)

2013-03-14

... 1625-AA00 Safety Zone; V. I. Carnival Finale; St. Thomas Harbor; St. Thomas, U.S. Virgin Islands AGENCY... establish a safety zone on the waters of St. Thomas Harbor in St. Thomas, U. S. Virgin Islands during the V... between 9 a.m. and 5 p.m., Monday through Friday, except federal holidays. The telephone number is 202-366...

Army Engineer Divers: First In Port-Au-Prince Harbor

Science.gov (United States)

2010-12-01

to the pile once the concrete was poured. This step was com- pleted in a few minutes for each pile. Last, a prefabricated wooden form was emplaced...September-December 201010 Engineer This prefabricated wooden form was emplaced around a rebar cage at the top of damaged piles to hold concrete until it...the mainland into the harbor. Waste from tugboats and sewage from the mainland compounded ecological hazards. The only alternative for the Army and

Environmental Assessment for Boston Harbor Maintenance Dredging, Boston, Massachusetts.

Science.gov (United States)

1981-12-01

Harbor was developed by Jerome et al (1966), Chesmore et al (1971) and Iwanowicz et al. (1973). The studies on the Lower Mystic River were concentrated in... Iwanowicz et al. (1973) and this data should be referred to for detailed information. Waters overlying the shellfish beds are contaminated by wastes...DMRP Technical Report DS-78-5, Environmental Laboratory, U.S. Army Engineer Waterways Experiment Station, Vicksburg, Mississippi. Iwanowicz , H. R., R D

33 CFR 334.840 - Waters of Lake Michigan south of Northerly Island at entrance to Burnham Park Yacht Harbor...

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Waters of Lake Michigan south of Northerly Island at entrance to Burnham Park Yacht Harbor, Chicago, Ill.; danger zone adjacent to airport on... Michigan south of Northerly Island at entrance to Burnham Park Yacht Harbor, Chicago, Ill.; danger zone...

33 CFR 334.85 - New York Harbor, adjacent to the Stapleton Naval Station, Staten Island, New York; restricted area.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false New York Harbor, adjacent to the Stapleton Naval Station, Staten Island, New York; restricted area. 334.85 Section 334.85 Navigation and... RESTRICTED AREA REGULATIONS § 334.85 New York Harbor, adjacent to the Stapleton Naval Station, Staten Island...

Impact of climate changes on the Santos Harbor, São Paulo State (Brazil

Directory of Open Access Journals (Sweden)

Paolo Alfredini

2013-12-01

Full Text Available Santos Harbor Area (SHA in Sao Paulo Coastline (Brazil is the most important marine cargo transfer terminal in the Southern Hemisphere. A long term relative tidal level variability assessment shows a consistent response to relative sea level rise. A wave data base Wave Watch III was compared with a long term wave data-base generated by the ERA40-ECMWF (2003, both local validated. The current bed level of SHA Outer Channel is -15.00 m (Chart Datum or, in abbreviation, CD, maintained by dredging. According to the cargo throughput forecast, in 2025, the Access Channel will have to be deepened to level of -17.00 m. The feasibility of that choice is discussed from a technical, economical and conceptual navigation point of view in that context. A data set found from a scale model of the whole area of Santos Bay, Estuary and nearby beaches, showed the impact of maritime climate changes upon the coastal area. In the previous researches developed by the authors, it was demonstrated that the wave climate, the tides and tidal currents affect harbor and coastal structures maintenance, beaches stability, tidal inlet, sediment transport, saline intrusion and wetlands. Considering the increasing of the sea hazards and the high values of the infrastructures in that coastline, it is necessary to mitigate the risks. Hence, based on the results obtained by the authors, are highlighted guidelines strategies suggested for Access Channels dimensions, wharves free-board, jetties dimensions, dredging rates, rigid and flexible littoral defenses and land protection against flooding (including wetlands.

Annexin A2 promotes the migration and invasion of human hepatocellular carcinoma cells in vitro by regulating the shedding of CD147-harboring microvesicles from tumor cells.

Directory of Open Access Journals (Sweden)

Wei Zhang

Full Text Available It has been reported that Annexin A2 (ANXA2 is up-regulated in hepatocellular carcinoma (HCC, but the roles of ANXA2 in the migration and invasion of HCC cells have not been determined. In this study, we found that ANXA2-specific siRNA (si-ANXA2 significantly inhibited the migration and invasion of HCC cells co-cultured with fibroblasts in vitro. In addition, the production of MMP-2 by fibroblasts cultured in supernatant collected from si-ANXA2-transfected HCC cells was notably down-regulated. ANXA2 was also found to be co-localized and co-immunoprecipitated with CD147. Further investigation revealed that the expression of ANXA2 in HCC cells affected the shedding of CD147-harboring membrane microvesicles, acting as a vehicle for CD147 in tumor-stromal interactions and thereby regulating the production of MMP-2 by fibroblasts. Together, these results suggest that ANXA2 enhances the migration and invasion potential of HCC cells in vitro by regulating the trafficking of CD147-harboring membrane microvesicles.

Wave Climate and Wave Response, Kawaihae Deep Draft Harbor, Island of Hawaii, Hawaii

National Research Council Canada - National Science Library

Thompson, Edward F; Demirbilek, Zeki; Briggs, Michael J

2006-01-01

Present and projected commercial activities in Kawaihae Deep Draft Harbor, Island of Hawaii, HI, indicate that a deeper basin and entrance channel and better protected berthing areas will be needed. The U.S...

Kernel Method Based Human Model for Enhancing Interactive Evolutionary Optimization

Science.gov (United States)

Zhao, Qiangfu; Liu, Yong

2015-01-01

A fitness landscape presents the relationship between individual and its reproductive success in evolutionary computation (EC). However, discrete and approximate landscape in an original search space may not support enough and accurate information for EC search, especially in interactive EC (IEC). The fitness landscape of human subjective evaluation in IEC is very difficult and impossible to model, even with a hypothesis of what its definition might be. In this paper, we propose a method to establish a human model in projected high dimensional search space by kernel classification for enhancing IEC search. Because bivalent logic is a simplest perceptual paradigm, the human model is established by considering this paradigm principle. In feature space, we design a linear classifier as a human model to obtain user preference knowledge, which cannot be supported linearly in original discrete search space. The human model is established by this method for predicting potential perceptual knowledge of human. With the human model, we design an evolution control method to enhance IEC search. From experimental evaluation results with a pseudo-IEC user, our proposed model and method can enhance IEC search significantly. PMID:25879050

Space Threat Warning: Foundation for Space Superiority, Avoiding a Space Pearl Harbor

National Research Council Canada - National Science Library

Burke, Alan W

2006-01-01

... have stated the US must avoid a space Pearl Harbor. This concern is due to the idea that a decrease in the perceived threat to space assets after the demise of the Soviet Union coupled with a competition for space resources has resulted...

33 CFR 117.213 - New Haven Harbor, Quinnipiac and Mill Rivers.

Science.gov (United States)

2010-07-01

... Mill Rivers. 117.213 Section 117.213 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Haven Harbor, Quinnipiac and Mill Rivers. The draws of the Tomlinson bridge, mile 0.0, the Ferry Street... bridge, mile 0.4 across Mill River, shall operate as follows: (a) The draws shall open on signal; except...

78 FR 18481 - Drawbridge Operation Regulations; Inner Harbor Navigation Canal, LA

Science.gov (United States)

2013-03-27

... regulation governing the operation of the US 90 (Danzinger) vertical lift span drawbridge across the Inner... Lift Span Bridge across the Inner Harbor Navigation Canal, mile 3.10 at New Orleans, Orleans Parish, Louisiana. The bridge has a vertical clearance of 50 feet above mean high water, elevation 5.0 feet Mean Sea...

An enhancement to Vynnycky's model for the all-vanadium redox flow battery

International Nuclear Information System (INIS)

Chen, Ching Liang; Yeoh, Hak Koon; Chakrabarti, Mohammed Harun

2014-01-01

Highlights: • Improvements are made on an existing 1-D stationary VRFB model. • Effects of species concentration and electrolyte flow rate are captured. • Predictions on charge-discharge curves are improved over existing 1-D model. - Abstract: An enhanced one-dimensional (1-D) stationary model for the all-vanadium redox flow battery (VRFB) is developed based on an existing 1-D model proposed by Vynnycky [Energy, 36 (2011): 2242 – 2256]. The enhanced model incorporates species conservation equations along with an advection term to describe the concentration changes in the porous electrodes. In addition, a complete Nernst equation, which accounts for proton concentrations in the VRFB is also included to improve the cell voltage prediction without using any arbitrary fitting contact voltage. The enhanced 1-D model is validated against experimental data from the literature and the ability of the model to predict the cell performance is investigated. The cell voltage prediction shows significant improvement over Vynnycky's 1-D model and also compares surprisingly well with higher-dimensional models. This enhanced 1-D model is also capable of capturing the cell performance at different electrolyte flow rates, especially evidenced by the polarization curves. Using both the power based and round-trip efficiencies, the optimal electrolyte flow rate for the VRFB can be determined. This enhanced 1-D model is expected to serve as a useful design tool for the development and optimization of VRFB systems

Discovering, analyzing and enhancing BPMN models using ProM

NARCIS (Netherlands)

Kalenkova, A.A.; Leoni, de M.; Aalst, van der W.M.P.; Limonad, L.; Weber, B.

2014-01-01

Process mining techniques relate observed behavior to modeled behavior, e.g., the automatic discovery of a process model based on an event log. Process mining is not limited to process discovery and also includes conformance checking and model enhancement. Conformance checking techniques are used to

Enhancement of speech signals - with a focus on voiced speech models

DEFF Research Database (Denmark)

Nørholm, Sidsel Marie

This thesis deals with speech enhancement, i.e., noise reduction in speech signals. This has applications in, e.g., hearing aids and teleconference systems. We consider a signal-driven approach to speech enhancement where a model of the speech is assumed and filters are generated based...... on this model. The basic model used in this thesis is the harmonic model which is a commonly used model for describing the voiced part of the speech signal. We show that it can be beneficial to extend the model to take inharmonicities or the non-stationarity of speech into account. Extending the model...

A solitary hyperfunctioning thyroid nodule harboring thyroid carcinoma: review of the literature.

Science.gov (United States)

Mirfakhraee, Sasan; Mathews, Dana; Peng, Lan; Woodruff, Stacey; Zigman, Jeffrey M

2013-05-04

Hyperfunctioning nodules of the thyroid are thought to only rarely harbor thyroid cancer, and thus are infrequently biopsied. Here, we present the case of a patient with a hyperfunctioning thyroid nodule harboring thyroid carcinoma and, using MEDLINE literature searches, set out to determine the prevalence of and characteristics of malignant "hot" nodules as a group. Historical, biochemical and radiologic characteristics of the case subjects and their nodules were compared to those in cases of benign hyperfunctioning nodules. A literature review of surgical patients with solitary hyperfunctioning thyroid nodules managed by thyroid resection revealed an estimated 3.1% prevalence of malignancy. A separate literature search uncovered 76 cases of reported malignant hot thyroid nodules, besides the present case. Of these, 78% were female and mean age at time of diagnosis was 47 years. Mean nodule size was 4.13 ± 1.68 cm. Laboratory assessment revealed T3 elevation in 76.5%, T4 elevation in 51.9%, and subclinical hyperthyroidism in 13% of patients. Histological diagnosis was papillary thyroid carcinoma (PTC) in 57.1%, follicular thyroid carcinoma (FTC) in 36.4%, and Hurthle cell carcinoma in 7.8% of patients. Thus, hot thyroid nodules harbor a low but non-trivial rate of malignancy. Compared to individuals with benign hyperfunctioning thyroid nodules, those with malignant hyperfunctioning nodules are younger and more predominantly female. Also, FTC and Hurthle cell carcinoma are found more frequently in hot nodules than in general. We were unable to find any specific characteristics that could be used to distinguish between malignant and benign hot nodules.

Buffalo Harbor Study. Preliminary Feasibility Report. Volume I. Main Report.

Science.gov (United States)

1983-04-01

to usually narrow strips of riparian vegetation, which is composed of various trees and shrubs of the Salix genus (willow), sumac, aspen, boxelder...reptiles were found. Species included, leopard frogs, snapping turtles, painted turtle, and garter snakes (SUNY Brockport: 1982). (7) Endangered Species...vessel traffic on the Buffalo River causes interrupted truck service. Firms have also cited snow removal as a problem. The harbor area road service

A crisis in waste management, economic vitality, and a coastal marine environment: Boston Harbor and Massachusetts Bay

Science.gov (United States)

Manheim, F.T.; Butman, B.

1994-01-01

Discharge of sewage sludge and effluent from 43 communities in the greater Boston metropolitan area has helped make the harbor one of the most polluted in the nation. As part of a court-mandated plan to end pollution of the harbor, effluent will no longer be discharged into the harbor, but instead, by 1995 it will be discharged into Massachusetts Bay through a record-long 15.34 km tunnel. By the year 2000 all of the sewage is scheduled to recive full secondary treatment. The public is concerned about long-term effects of the new ocean outfall on the environment, including Cape Cod Bay and Stellwagen Bank, which is an important habitat for whales and a newly designated national marine sanctuary. The bay has been additionally stressed by dumping of low-level radioactive and other hazardous wastes during the 1950s and 1960s. -from Authors

Cloning and Sequencing of Protein Kinase cDNA from Harbor Seal (Phoca vitulina Lymphocytes

Directory of Open Access Journals (Sweden)

Jennifer C. C. Neale

2004-01-01

Full Text Available Protein kinases (PKs play critical roles in signal transduction and activation of lymphocytes. The identification of PK genes provides a tool for understanding mechanisms of immunotoxic xenobiotics. As part of a larger study investigating persistent organic pollutants in the harbor seal and their possible immunomodulatory actions, we sequenced harbor seal cDNA fragments encoding PKs. The procedure, using degenerate primers based on conserved motifs of human protein tyrosine kinases (PTKs, successfully amplified nine phocid PK gene fragments with high homology to human and rodent orthologs. We identified eight PTKs and one dual (serine/threonine and tyrosine kinase. Among these were several PKs important in early signaling events through the B- and T-cell receptors (FYN, LYN, ITK and SYK and a MAP kinase involved in downstream signal transduction. V-FGR, RET and DDR2 were also expressed. Sequential activation of protein kinases ultimately induces gene transcription leading to the proliferation and differentiation of lymphocytes critical to adaptive immunity. PKs are potential targets of bioactive xenobiotics, including persistent organic pollutants of the marine environment; characterization of these molecules in the harbor seal provides a foundation for further research illuminating mechanisms of action of contaminants speculated to contribute to large-scale die-offs of marine mammals via immunosuppression.

Quantification of total mercury in liver and heart tissue of Harbor Seals (Phoca vitulina) from Alaska USA

International Nuclear Information System (INIS)

Marino, Kady B.; Hoover-Miller, Anne; Conlon, Suzanne; Prewitt, Jill; O'Shea, Stephen K.

2011-01-01

This study quantified the Hg levels in the liver (n=98) and heart (n=43) tissues of Harbor Seals (Phoca vitulina) (n=102) harvested from Prince William Sound and Kodiak Island Alaska. Mercury tissue dry weight (dw) concentrations in the liver ranged from 1.7 to 393 ppm dw, and in the heart from 0.19 to 4.99 ppm dw. Results of this study indicate liver and heart tissues' Hg ppm dw concentrations significantly increase with age. Male Harbor Seals bioaccumulated Hg in both their liver and heart tissues at a significantly faster rate than females. The liver Hg bioaccumulation rates between the harvest locations Kodiak Island and Prince William Sound were not found to be significantly different. On adsorption Hg is transported throughout the Harbor Seal's body with the partition coefficient higher for the liver than the heart. No significant differences in the bio-distribution (liver:heart Hg ppm dw ratios (n=38)) values were found with respect to either age, sex or geographic harvest location. In this study the age at which Hg liver and heart bioaccumulation levels become significantly distinct in male and female Harbor Seals were identified through a Tukey's analysis. Of notably concern to human health was a male Harbor Seal's liver tissue harvested from Kodiak Island region. Mercury accumulation in this sample tissue was determined through a Q-test to be an outlier, having far higher Hg concentrarion (liver 392 Hg ppm dw) than the general population sampled. - Highlights: ► Mercury accumulation in the liver and heart of seals exceed food safety guidelines. ► Accumulation rate is greater in males than females with age. ► Liver mercury accumulation is greater than in the heart tissues. ► Mercury determination by USA EPA Method 7473 using thermal decomposition.

Model calculation of the scanned field enhancement factor of CNTs

International Nuclear Information System (INIS)

Ahmad, Amir; Tripathi, V K

2006-01-01

The field enhancement factor of a carbon nanotube (CNT) placed in a cluster of CNTs is smaller than an isolated CNT because the electric field on one tube is screened by neighbouring tubes. This screening depends on the length of the CNTs and the spacing between them. We have derived an expression to compute the field enhancement factor of CNTs under any positional distribution of CNTs using a model of a floating sphere between parallel anode and cathode plates. Using this expression we can compute the field enhancement factor of a CNT in a cluster (non-uniformly distributed CNTs). This expression is used to compute the field enhancement factor of a CNT in an array (uniformly distributed CNTs). Comparison has been shown with experimental results and existing models

Novel B19-like parvovirus in the brain of a harbor seal

NARCIS (Netherlands)

R. Bodewes (Rogier); A.R. García (Ana Rubio); L.C.M. Wiersma (Lidewij); S. Getu (Sarah); M. Beukers (Martijn); C.M.E. Schapendonk (Claudia); P.R.W.A. van Run (Peter); M.W.G. van de Bildt (Marco); M.J. Poen (Marjolein); N. Osinga (Nynke); G.J. Sánchez Contreras (Guillermo); T. Kuiken (Thijs); S.L. Smits (Saskia); A.D.M.E. Osterhaus (Albert)

2013-01-01

textabstractUsing random PCR in combination with next-generation sequencing, a novel parvovirus was detected in the brain of a young harbor seal (Phoca vitulina) with chronic non-suppurative meningo-encephalitis that was rehabilitated at the Seal Rehabilitation and Research Centre (SRRC) in the

Validating a Technology Enhanced Student-Centered Learning Model

Science.gov (United States)

Kang, Myunghee; Hahn, Jungsun; Chung, Warren

2015-01-01

The Technology Enhanced Student Centered Learning (TESCL) Model in this study presents the core factors that ensure the quality of learning in a technology-supported environment. Although the model was conceptually constructed using a student-centered learning framework and drawing upon previous studies, it should be validated through real-world…

The Use of the Kano Model to Enhance Customer Satisfaction

Directory of Open Access Journals (Sweden)

Južnik Rotar Laura

2017-12-01

Full Text Available Background/purpose: The interest of measuring customer satisfaction is reflected in its ability to gain customer loyalty, enhance favourable word of mouth, lead to repeat purchases and improve a company’s market share and profitability. The issue of integrating the Kano model of customer satisfaction with other models and tools to support development or improvement of a product, or to determine market strategies, is relatively unexplored in the Slovenian sector. This research aims to construct the Kano model in order to enhance customer satisfaction in the case of home appliances.

Onondaga Lake Inner Harbor Dredging Design Project, Syracuse, New York: Final Design Memorandum

National Research Council Canada - National Science Library

1997-01-01

.... The sponsor is the New York State Canal Corporation. The design includes deepening the Inner Harbor channel and a portion of the terminal slip area to a depth of 10 feet below Low Water Datum (LWD...

Inshore Survey Results: Approaches to New York Harbor, Fall 1955 (NODC Accession 7000294)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — A current survey in the approaches to New York Harbor was initiated 06 September 1955 and continued through 04 November 1955. This survey was conducted in accordance...

The historical significance of anaesthesia events at Pearl Harbor.

Science.gov (United States)

Crowhurst, Ja

2014-07-01

Up to the end of World War II, less than 10% of the general anaesthetics administered was with intravenous barbiturates. The remaining 90% of anaesthetics given in the USA were with diethyl ether. In the United Kingdom and elsewhere, chloroform was also popular. Diethyl ether administration was a relatively safe and simple procedure, often delegated to nurses or junior doctors with little or no specific training in anaesthesia. During the Japanese attack on the US bases at Pearl Harbor, with reduced stocks of diethyl ether available, intravenous Sodium Pentothal(®), a most 'sophisticated and complex' drug, was used with devastating effects in many of those hypovolaemic, anaemic and septic patients. The hazards of spinal anaesthesia too were realised very quickly. These effects were compounded by the dearth of trained anaesthetists. This paper presents the significance of the anaesthesia tragedies at Pearl Harbor, and the discovery in the next few years of many other superior drugs that caused medical and other health professionals to realise that anaesthesia needed to be a specialist medical discipline in its own right. Specialist recognition, aided by the foundation of the National Health Service in the UK, the establishment of Faculties of Anaesthesia and appropriate training in pharmacology, physiology and other sciences soon followed. Modern anaesthesiology, as we understand it today, was born and a century or more of ether anaesthesia finally ceased.

75 FR 53574 - Safety Zone; Fireworks Displays, Potomac River, National Harbor, MD

Science.gov (United States)

2010-09-01

...-AA00 Safety Zone; Fireworks Displays, Potomac River, National Harbor, MD AGENCY: Coast Guard, DHS... safety of life on navigable waters during five fireworks displays launched from a discharge barge located... necessary to protect persons and vessels against the hazards associated with a fireworks display on...

Evaluation of a Mathematical Model for Digital Image Enhancement.

Science.gov (United States)

Geha, Hassem; Nasseh, Ibrahim; Noujeim, Marcel

2015-01-01

The purpose of this study is to compare the detected number of holes on a stepwedge on images resulting from the application of the 5th degree polynomial model compared to the images resulting from the application of linear enhancement. Material and Methods : A 10-step aluminum step wedge with holes randomly drilled on each step was exposed with three different kVp and five exposure times per kVp on a Schick33(®) sensor. The images were enhanced by brightness/contrast adjustment, histogram equalization and with the 5th degree polynomial model and compared to the original non-enhanced images by six observers in two separate readings. Results : There was no significant difference between the readers and between the first and second reading. There was a significant three-factor interaction among Method, Exposure time, and kVp in detecting holes. The overall pattern was: "Poly" results in the highest counts, "Original" in the lowest counts, with "B/C" and "Equalized" intermediate. Conclusion : The 5th degree polynomial model showed more holes when compared to the other modalities.

A generative modeling approach to connectivity-Electrical conduction in vascular networks

DEFF Research Database (Denmark)

Hald, Bjørn Olav

2016-01-01

The physiology of biological structures is inherently dynamic and emerges from the interaction and assembly of large collections of small entities. The extent of coupled entities complicates modeling and increases computational load. Here, microvascular networks are used to present a novel...... to synchronize vessel tone across the vast distances within a network. We hypothesize that electrical conduction capacity is delimited by the size of vascular structures and connectivity of the network. Generation and simulation of series of dynamical models of electrical spread within vascular networks...... of different size and composition showed that (1) Conduction is enhanced in models harboring long and thin endothelial cells that couple preferentially along the longitudinal axis. (2) Conduction across a branch point depends on endothelial connectivity between branches. (3) Low connectivity sub...

Enhanced Contact Graph Routing (ECGR) MACHETE Simulation Model

Science.gov (United States)

Segui, John S.; Jennings, Esther H.; Clare, Loren P.

2013-01-01

Contact Graph Routing (CGR) for Delay/Disruption Tolerant Networking (DTN) space-based networks makes use of the predictable nature of node contacts to make real-time routing decisions given unpredictable traffic patterns. The contact graph will have been disseminated to all nodes before the start of route computation. CGR was designed for space-based networking environments where future contact plans are known or are independently computable (e.g., using known orbital dynamics). For each data item (known as a bundle in DTN), a node independently performs route selection by examining possible paths to the destination. Route computation could conceivably run thousands of times a second, so computational load is important. This work refers to the simulation software model of Enhanced Contact Graph Routing (ECGR) for DTN Bundle Protocol in JPL's MACHETE simulation tool. The simulation model was used for performance analysis of CGR and led to several performance enhancements. The simulation model was used to demonstrate the improvements of ECGR over CGR as well as other routing methods in space network scenarios. ECGR moved to using earliest arrival time because it is a global monotonically increasing metric that guarantees the safety properties needed for the solution's correctness since route re-computation occurs at each node to accommodate unpredicted changes (e.g., traffic pattern, link quality). Furthermore, using earliest arrival time enabled the use of the standard Dijkstra algorithm for path selection. The Dijkstra algorithm for path selection has a well-known inexpensive computational cost. These enhancements have been integrated into the open source CGR implementation. The ECGR model is also useful for route metric experimentation and comparisons with other DTN routing protocols particularly when combined with MACHETE's space networking models and Delay Tolerant Link State Routing (DTLSR) model.

Applicability of numerical model for seabed topography changes by tsunami flow. Analysis of formulae for sediment transport and simulations in a rectangular harbor

International Nuclear Information System (INIS)

Matsuyama, Masafumi

2009-01-01

Characteristics of formulae for bed-load transport and pick-up rate in suspended transport are investigated in order to clarify the impact on seabed topography changes by tsunami flow. The impact by bed-load transport was depended on Froude number and water surface slope. Bed-load transport causes deposition under Fr 6/7 at face front of tsunami wave. Pick-up rate has more predominant influences for seabed topography changes than that of one brought by bed-load transport. 2-D Numerical simulations with formulae by Ikeno et.al were carried out to simulate topography changes around harbor by tsunami flow in the flume. The result indicated that the numerical model is more applicable than a numerical model with previous formulae for estimation of deposit and erosion by topography changes. It is for this reason that the formula of pick-up rate is adaptable for wide-range diameter of sand, from 0.08mm to 0.2mm. Upper limit of suspended sediment concentration is needed to set due to avoid overlarge concentration in the numerical model. Comparison between numerical results in a real scale with 1% and 5% upper limits clearly shows topography changes have a deep relevance with the upper limit value. The upper limit value is one of dominant factors for evaluating seabed topography changes by the 2-D Numerical simulations with the formulae by Ikeno et.al in a real scale. (author)

75 FR 5907 - Safety Zone; Dive Platform, Pago Pago Harbor, American Samoa

Science.gov (United States)

2010-02-05

... platform vessel in Pago Pago Harbor, American Samoa, while diving operations are under way in and around the CHEHALIS wreck. The safety zone is necessary to protect other vessels and the general public from... Pago, American Samoa. Today, the CHEHALIS wreck remains a potential pollution threat to the environment...

Dutch Harbor, Alaska Coastal Digital Elevation Model

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NOAA's National Geophysical Data Center (NGDC) is building high-resolution digital elevation models (DEMs) for select U.S. coastal regions. These integrated...

Interim Feasibility Report and Draft Environmental Impact Statement, Grays Harbor, Chehalis and Hoquiam Rivers, Washington, Channel Improvements for Navigation.

Science.gov (United States)

1982-06-01

community depends on the continued descent of organic materials from the overlying waters for nourishment. The Dungeness crab ( Cancer magister) is an...immediately north and south of Grays Harbor. EIS-34 o Crabs. Dungeness crabs ( Cancer magister) are abundant in Grays Harbor, though most are smaller...Cooper Cultural Resources Mama Seattle District, Evaluation Corps of Engineers Endangered Species Brunner Seattle District, Evaluation Corps of Engineers

Radiation enhanced conduction in insulators: computer modelling

International Nuclear Information System (INIS)

Fisher, A.J.

1986-10-01

The report describes the implementation of the Klaffky-Rose-Goland-Dienes [Phys. Rev. B.21 3610,1980] model of radiation-enhanced conduction and describes the codes used. The approach is demonstrated for the data for alumina of Pells, Buckley, Hill and Murphy [AERE R.11715, 1985]. (author)

Environmental Assessment for Waterfront Facilities Maintenance and Improvements, Pearl Harbor Naval Complex, Oahu, Hawaii

National Research Council Canada - National Science Library

2005-01-01

Commander, Navy Region Hawaii (CNRH) proposes to repair, maintain, and improve waterfront berthing and maintenance facilities for ships and submarines on an as-needed basis within the Pearl Harbor Naval Complex (PHNC...

USign--a security enhanced electronic consent model.

Science.gov (United States)

Li, Yanyan; Xie, Mengjun; Bian, Jiang

2014-01-01

Electronic consent becomes increasingly popular in the healthcare sector given the many benefits it provides. However, security concerns, e.g., how to verify the identity of a person who is remotely accessing the electronic consent system in a secure and user-friendly manner, also arise along with the popularity of electronic consent. Unfortunately, existing electronic consent systems do not pay sufficient attention to those issues. They mainly rely on conventional password based authentication to verify the identity of an electronic consent user, which is far from being sufficient given that identity theft threat is real and significant in reality. In this paper, we present a security enhanced electronic consent model called USign. USign enhances the identity protection and authentication for electronic consent systems by leveraging handwritten signatures everyone is familiar with and mobile computing technologies that are becoming ubiquitous. We developed a prototype of USign and conducted preliminary evaluation on accuracy and usability of signature verification. Our experimental results show the feasibility of the proposed model.

Lactic Acid Recovery in Electro-Enhanced Dialysis: Modelling and Validation

DEFF Research Database (Denmark)

Prado Rubio, Oscar Andres; Jørgensen, Sten Bay; Jonsson, Gunnar Eigil

2009-01-01

and migration across the boundary layers and membranes. The model is validated for Donnan dialysis recovery of different monoprotic carboxylic acids. Simulations are used to evaluate the potential enhancement of lactate fluxes under current load conditions, referred as Electro-Enhanced Dialysis operation...

Female harbor seal (Phoca vitulina) behavioral response to playbacks of underwater male acoustic advertisement displays.

Science.gov (United States)

Matthews, Leanna P; Blades, Brittany; Parks, Susan E

2018-01-01

During the breeding season, male harbor seals ( Phoca vitulina ) make underwater acoustic displays using vocalizations known as roars. These roars have been shown to function in territory establishment in some breeding areas and have been hypothesized to be important for female choice, but the function of these sounds remains unresolved. This study consisted of a series of playback experiments in which captive female harbor seals were exposed to recordings of male roars to determine if females respond to recordings of male vocalizations and whether or not they respond differently to roars from categories with different acoustic characteristics. The categories included roars with characteristics of dominant males (longest duration, lowest frequency), subordinate males (shortest duration, highest frequency), combinations of call parameters from dominant and subordinate males (long duration, high frequency and short duration, low frequency), and control playbacks of water noise and water noise with tonal signals in the same frequency range as male signals. Results indicate that overall females have a significantly higher level of response to playbacks that imitate male vocalizations when compared to control playbacks of water noise. Specifically, there was a higher level of response to playbacks representing dominant male vocalization when compared to the control playbacks. For most individuals, there was a greater response to playbacks representing dominant male vocalizations compared to playbacks representing subordinate male vocalizations; however, there was no statistical difference between those two playback types. Additionally, there was no difference between the playbacks of call parameter combinations and the controls. Investigating female preference for male harbor seal vocalizations is a critical step in understanding the harbor seal mating system and further studies expanding on this captive study will help shed light on this important issue.

Remembering Pearl Harbor: The USS Arizona Memorial. Teaching with Historic Places.

Science.gov (United States)

Vierra, John, Jr.

This lesson describes and discusses the submerged remains of the battleship USS Arizona which rests on the silt of Pearl Harbor (Hawaii), just as it had settled on December 7, 1941, the day Japan attacked the U.S. fleet and began the Pacific battles of World War II. The lesson is based on the National Register of Historic Places registration file,…

33 CFR 165.709 - Security Zone; Charleston Harbor, Cooper River, South Carolina.

Science.gov (United States)

2010-07-01

..., Cooper River, South Carolina. 165.709 Section 165.709 Navigation and Navigable Waters COAST GUARD... § 165.709 Security Zone; Charleston Harbor, Cooper River, South Carolina. (a) Regulated area. The Coast Guard is establishing a fixed security zone on all waters of the Cooper River, bank-to-bank and surface...

78 FR 68002 - Safety Zone for Fireworks Display, Baltimore Harbor, Baltimore, MD

Science.gov (United States)

2013-11-13

... 1625-AA00 Safety Zone for Fireworks Display, Baltimore Harbor, Baltimore, MD AGENCY: Coast Guard, DHS... safety of life on navigable waters during a fireworks display launched from a barge located in Baltimore... rule involves a fireworks display associated with a New Year's Eve event that will take place in...

Simulating Surface-Enhanced Hyper-Raman Scattering Using Atomistic Electrodynamics-Quantum Mechanical Models.

Science.gov (United States)

Hu, Zhongwei; Chulhai, Dhabih V; Jensen, Lasse

2016-12-13

Surface-enhanced hyper-Raman scattering (SEHRS) is the two-photon analogue of surface-enhanced Raman scattering (SERS), which has proven to be a powerful tool to study molecular structures and surface enhancements. However, few theoretical approaches to SEHRS exist and most neglect the atomistic descriptions of the metal surface and molecular resonance effects. In this work, we present two atomistic electrodynamics-quantum mechanical models to simulate SEHRS. The first is the discrete interaction model/quantum mechanical (DIM/QM) model, which combines an atomistic electrodynamics model of the nanoparticle with a time-dependent density functional theory description of the molecule. The second model is a dressed-tensors method that describes the molecule as a point-dipole and point-quadrupole object interacting with the enhanced local field and field-gradients (FG) from the nanoparticle. In both of these models, the resonance effects are treated efficiently by means of damped quadratic response theory. Using these methods, we simulate SEHRS spectra for benzene and pyridine. Our results show that the FG effects in SEHRS play an important role in determining both the surface selection rules and the enhancements. We find that FG effects are more important in SEHRS than in SERS. We also show that the spectral features of small molecules can be accurately described by accounting for the interactions between the molecule and the local field and FG of the nanoparticle. However, at short distances between the metal and molecule, we find significant differences in the SEHRS enhancements predicted using the DIM/QM and the dressed-tensors methods.

Benthic foraminifera distribution in high polluted sediments from Niterói Harbor (Guanabara Bay), Rio de Janeiro, Brazil

OpenAIRE

Claudia G. Vilela; Daniele S. Batista; José A. Batista-Neto; Mirian Crapez; John J. Mcallister

2004-01-01

Dockyards and harbors are recognized as being important locations where sediment-associated pollutants can accumulate, which constitutes an environmental risk to aquatic life due to potential uptake and accumulation of heavy metals in the biota. The aim of this paper is to assess the concentrations and the effects of some heavy metals in the benthic foraminifera assemblage in Niterói Harbor. Low concentrations in the benthic foraminifera as well as the dominance of indicative species such as ...

ФC31 Integrase-Mediated Isolation and Characterization of Novel Safe Harbors for Transgene Expression in the Pig Genome

Science.gov (United States)

Bi, Yanzhen; Hua, Zaidong; Ren, Hongyan; Zhang, Liping; Xiao, Hongwei; Liu, Ximei; Hua, Wenjun; Mei, Shuqi; Molenaar, Adrian; Laible, Götz; Zheng, Xinmin

2018-01-01

Programmable nucleases have allowed the rapid development of gene editing and transgenics, but the technology still suffers from the lack of predefined genetic loci for reliable transgene expression and maintenance. To address this issue, we used ФC31 integrase to navigate the porcine genome and identify the pseudo attP sites suitable as safe harbors for sustained transgene expression. The combined ФC31 integrase mRNA and an enhanced green fluorescence protein (EGFP) reporter donor were microinjected into one-cell zygotes for transgene integration. Among the resulting seven EGFP-positive piglets, two had transgene integrations at pseudo attP sites, located in an intergenic region of chromosome 1 (chr1-attP) and the 6th intron of the TRABD2A gene on chromosome 3 (chr3-attP), respectively. The integration structure was determined by TAIL-PCR and Southern blotting. Primary fibroblast cells were isolated from the two piglets and examined using fluorescence-activated cell sorting (FACS) and enzyme-linked immunosorbent assay (ELISA), which demonstrated that the chr1-attP site was more potent than chr3-attP site in supporting the EGFP expression. Both piglets had green feet under the emission of UV light, and pelleted primary fibroblast cells were green-colored under natural light, corroborating that the two pseudo attP sites are beneficial to transgene expression. The discovery of these two novel safe harbors for robust and durable transgene expression will greatly facilitate the use of transgenic pigs for basic, biomedical and agricultural studies and applications. PMID:29300364

Red mangrove (Rhizophora mangle) reproduction and seedling colonization after Hurricane Charley: Comparisons of Charlotte Harbor and Tampa Bay

Science.gov (United States)

Proffitt, C.E.; Milbrandt, E.C.; Travis, S.E.

2006-01-01

Reproductive aspects of life history are known to be important in recovery following disturbance in many plant species although this has not been well studied in mangroves. Hurricane Charley devastated large areas of mangroves in Charlotte Harbor, Florida, in August 2004. We surveyed 6 forests in Charlotte Harbor (2002, 2003, and 2005) and 16 in Tampa Bay, Florida (2001, 2002, 2003, and 2005) for total numbers of reproducing trees and trees heterozygotic for albinism that produce both normal and albino propagules. Tree size (estimated height and diameter at breast height) was also recorded for sentinel heterozygotic trees. Total number of reproducing trees km-1 was used as an index of reproductive output of the population, and deviation from the 3:1 (normal:albino propagules) ratio on heterozygotic trees expected with 100% selfing was used to estimate outcrossing. Numbers of Rhizophora mangle reproducing trees km-1 of shoreline in Charlotte Harbor were reduced by an order of magnitude following Hurricane Charley, while numbers of reproducing trees in Tampa Bay were similar to those of previous years. Reduced reproduction in Charlotte Harbor was accompanied by fewer new recruits in plots on Sanibel and Captiva Islands. Numbers of new recruits after the storm also tended to be fewer in plots where canopy loss was greater. More new recruits occurred in sites that had higher densities of pre-storm Rhizophora seedlings and greater relative dominance by Rhizophora. Outcrossing of sentinel trees was 2.5 times greater in Charlotte Harbor (mean site-1 = 33.6 ?? 6.7%; with 17% of forest sites completely selfing) than in Tampa Bay (mean site-1 = 13.4 ?? 4.7%; with 40% of sites completely selfing), although the implications for seedling recruitment of this difference are not known. ?? 2006 Estuarine Research Federation.

The effect of wave current interactions on the storm surge and inundation in Charleston Harbor during Hurricane Hugo 1989

Science.gov (United States)

Xie, Lian; Liu, Huiqing; Peng, Machuan

The effects of wave-current interactions on the storm surge and inundation induced by Hurricane Hugo in and around the Charleston Harbor and its adjacent coastal regions are examined by using a three-dimensional (3-D) wave-current coupled modeling system. The 3-D storm surge and inundation modeling component of the coupled system is based on the Princeton ocean model (POM), whereas the wave modeling component is based on the third-generation wave model, simulating waves nearshore (SWAN). The results indicate that the effects of wave-induced surface, bottom, and radiation stresses can separately or in combination produce significant changes in storm surge and inundation. The effects of waves vary spatially. In some areas, the contribution of waves to peak storm surge during Hurricane Hugo reached as high as 0.76 m which led to substantial changes in the inundation and drying areas simulated by the storm surge model.

Enhancing Students’ Communication Skills Through Treffinger Teaching Model

Directory of Open Access Journals (Sweden)

Idrus Alhaddad

2015-10-01

Full Text Available This research aims to investigate, compare, and describe the achievement and enhancement of students’ mathematical communication skills (MCS. It based on the prior mathematical knowledge (PMK category (high, medium and low by using Treffinger models (TM and conventional learning (CL. This research is an experimental study with the population of all students of Mathematics Education Department who took Discrete Mathematics subject matter of one university in the city of Ternate. The results show that (1 the achievement and enhancement of MCS students that used TM are higher than the students learning using CL; (2 Based on the categories of PMK, the achievement and enhancement of MCS of students using TM are also higher than those learning with CL; and (3 There was no interaction effect between learning (TM and CL and PMK to the achievement and enhancement of MCS of the students

Enhanced surrogate models for statistical design exploiting space mapping technology

DEFF Research Database (Denmark)

Koziel, Slawek; Bandler, John W.; Mohamed, Achmed S.

2005-01-01

We present advances in microwave and RF device modeling exploiting Space Mapping (SM) technology. We propose new SM modeling formulations utilizing input mappings, output mappings, frequency scaling and quadratic approximations. Our aim is to enhance circuit models for statistical analysis...

Enhanced angular overlap model for nonmetallic f -electron systems

Science.gov (United States)

Gajek, Z.

2005-07-01

An efficient method of interpretation of the crystal field effect in nonmetallic f -electron systems, the enhanced angular overlap model (EAOM), is presented. The method is established on the ground of perturbation expansion of the effective Hamiltonian for localized electrons and first-principles calculations related to available experimental data. The series of actinide compounds AO2 , oxychalcogenides AOX , and dichalcogenides UX2 where X=S ,Se,Te and A=U ,Np serve as probes of the effectiveness of the proposed method. An idea is to enhance the usual angular overlap model with ab initio calculations of those contributions to the crystal field potential, which cannot be represented by the usual angular overlap model (AOM). The enhancement leads to an improved fitting and makes the approach intrinsically coherent. In addition, the ab initio calculations of the main, AOM-consistent part of the crystal field potential allows one to fix the material-specific relations for the EAOM parameters in the effective Hamiltonian. Consequently, the electronic structure interpretation based on EAOM can be extended to systems of the lowest point symmetries or/and deficient experimental data. Several examples illustrating the promising capabilities of EAOM are given.

Enhanced Tunnelling Models for Child Universe Formation

CERN Document Server

Ansoldi, S; Shilon, I

2015-01-01

Starting from a recently proposed model that allows for an enhanced rate of child universe production under generic conditions, we elaborate on refinements that may allow for non-singular initial configurations. A possibility to treat both, the initial state and the tunnelling beyond the semiclassical level will also be introduced.

Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses.

Science.gov (United States)

Paudel, Sarita; Easwaran, Maheswaran; Jang, Hyun; Jung, Ho-Kyoung; Kim, Joo-Hun; Shin, Hyun-Jin

2016-07-15

In this study, we evaluated the immune responses of avian metapneumovirus harboring chicken Fc molecule. Stable Vero cells expressing chicken Fc chimera on its surface (Vero-cFc) were established, and we confirmed that aMPV grown in Vero-cFc incorporated host derived chimera Fc into the aMPV virions. Immunization of chicken with aMPV-cFc induced higher level of antibodies and inflammatory cytokines; (Interferon (IFN)-γ and Interleukin (IL)-1β) compared to those of aMPV. The increased levels of antibodies and inflammatory cytokines in chicken immunized with aMPV-cFc were statistically significantly (p<0.05) to that of aMPV and control. The aMPV-cFc group also generated the highest neutralizing antibody response. After challenges, chickens immunized with aMPV-cFc showed much less pathological signs in nasal turbinates and trachea so that we could confirm aMPV-cFc induced higher protection than that of aMPV. The greater ability of aMPV harboring chicken Fc to that of aMPV presented it as a possible vaccine candidate. Copyright © 2016 Elsevier B.V. All rights reserved.

Environmental Assessment for Building 88 Interior Demolition, Pearl Harbor Naval Complex, O'ahu, Hawaii

National Research Council Canada - National Science Library

2005-01-01

...) and ancillary equipment from the interior of Building 88, a former lubricating oil storage facility, and the related exposed piping underneath Mike wharves M3 and M4 at Merry Point, Pearl Harbor Naval Complex...

Nutritionally Enhanced Food Crops; Progress and Perspectives

Directory of Open Access Journals (Sweden)

Kathleen L. Hefferon

2015-02-01

Full Text Available Great progress has been made over the past decade with respect to the application of biotechnology to generate nutritionally improved food crops. Biofortified staple crops such as rice, maize and wheat harboring essential micronutrients to benefit the world’s poor are under development as well as new varieties of crops which have the ability to combat chronic disease. This review discusses the improvement of the nutritional status of crops to make a positive impact on global human health. Several examples of nutritionally enhanced crops which have been developed using biotechnological approaches will be discussed. These range from biofortified crops to crops with novel abilities to fight disease. The review concludes with a discussion of hurdles faced with respect to public perception, as well as directions of future research and development for nutritionally enhanced food crops.

Application of heterogeneous multiple camera system with panoramic capabilities in a harbor environment

NARCIS (Netherlands)

Schwering, P.B.W.; Lensen, H.A.; Broek, S.P. van den; Hollander, R.J.M. den; Mark, W. van der; Bouma, H.; Kemp, R.A.W.

2009-01-01

In a harbor environment threats like explosives-packed rubber boats, mine-carrying swimmers and divers must be detected in an early stage. This paper describes the integration and use of a heterogeneous multiple camera system with panoramic observation capabilities for detecting these small vessels

33 CFR 165.114 - Safety and Security Zones: Escorted Vessels-Boston Harbor, Massachusetts.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Safety and Security Zones... COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION... Guard District § 165.114 Safety and Security Zones: Escorted Vessels—Boston Harbor, Massachusetts. (a...

77 FR 11434 - Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD

Science.gov (United States)

2012-02-27

...-AA00 Safety Zone; Patapsco River, Northwest and Inner Harbors, Baltimore, MD AGENCY: Coast Guard, DHS... intended route immediately prior to, during, and following the scheduled towing evolution, vessel traffic... made to the maritime community via marine information broadcasts so mariners may adjust their plans...

Aerial Survey Trend Counts of Harbor Seals in Coastal Alaska (1984-2006) - ADF&G

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Aerial surveys were conducted during 1983–2006 in the Ketchikan, Sitka, Kodiak, and Bristol Bay areas of Alaska to estimate trends in abundance of harbor seals.

Contaminant transport and accumulation in Massachusetts Bay and Boston Harbor; a summary of U.S. Geological Survey studies

Science.gov (United States)

Butman, Bradford; Bothner, Michael H.; Hathaway, J.C.; Jenter, H.L.; Knebel, H.J.; Manheim, F.T.; Signell, R.P.

1992-01-01

The U.S. Geological Survey (USGS) is conducting studies in Boston Harbor, Massachusetts Bay, and Cape Cod Bay designed to define the geologic framework of the region and to understand the transport and accumulation of contaminated sediments. The region is being studied because of environmental problems caused by the introduction of wastes for a long time, because a new ocean outfall (to begin operation in 1995) will change the location for disposal of treated Boston sewage from Boston Harbor into Massachusetts Bay, and because of the need to understand the transport of sediments and associated contaminants in order to address a wide range of management questions. The USGS effort complements and is closely coordinated with the research and monitoring studies supported by the Massachusetts Environmental Trust, the Massachusetts Bays Program, and by the Massachusetts Water Resources Authority. The USGS study includes (1) geologic mapping, (2) circulation studies, (3) long-term current and sediment transport observations, (4) measurements of contaminant inventories and rates of sediment mixing and accumulation, (5) circulation modeling, (6) development of a contaminated sediments data base, and (7) information exchange. A long-term objective of the program is to develop a predictive capability for sediment transport and accumulation.

Safe Harbor Legislation for Juvenile Victims of Sex Trafficking: A Myopic View of Improvements in Practice

Directory of Open Access Journals (Sweden)

Kimberly Mehlman-Orozco

2015-02-01

Full Text Available Current social and political realties have focused attention on human trafficking in the United States. Although new mechanisms for criminalizing offenders and protecting victims are increasingly funded and implemented across the country, empirical exploration into the efficacy of these interventions is lacking. This article uses yearly count data on juvenile prostitution arrests aggregated at the state level to explore the criminalization of commercial sexually exploited children post safe harbor policy implementation. Preliminary data from four states suggests that the passage of safe harbor laws may not reduce the number of juveniles arrested for prostitution crimes. Implications for future research are discussed.

Phaeobacter inhibens from the Roseobacter clade has an environmental niche as a surface colonizer in harbors

DEFF Research Database (Denmark)

Gram, Lone; Rasmussen, Bastian Barker; Wemheuer, Bernd

2015-01-01

in the environment is to date unknown. Here, we report on the repeated isolation of Phaeobacter inhibens strains from a marine environment (harbors) not related to aquaculture. Based on phenotype and 16S rRNA gene sequence similarity, a total of 64 P. inhibens strains were identified from 35 samples (eukaryotic...... and used for amplification of 16S rRNA. P. inhibens was detected in five of these samples, all of which were biofilm samples, by pyrotag-sequencing at a prevalence of 0.02–0.68% of the prokaryotic community. The results indicated that P. inhibens had a niche in biofilms of fouled surfaces in harbor areas...

33 CFR 165.150 - New Haven Harbor, Quinnipiac River, Mill River.

Science.gov (United States)

2010-07-01

... River, Mill River. 165.150 Section 165.150 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... New Haven Harbor, Quinnipiac River, Mill River. (a) The following is a regulated navigation area: The... 303°T to point D at the west bank of the mouth of the Mill River 41°18′05″ N, 72°54′23″ W thence south...

NifH- Harboring Bacterial Community Composition Across an Alaskan Permafrost Thaw Gradient

Directory of Open Access Journals (Sweden)

Christopher Ryan Penton

2016-11-01

Full Text Available Since nitrogen (N is often limiting in permafrost soils, we investigated the N2-fixing genetic potential and the inferred taxa harboring those genes by sequencing nifH gene fragments in samples taken along a permafrost thaw gradient in an Alaskan boreal soil. Samples from minimally, moderately and extensively thawed sites were taken to a depth of 79 cm to encompass zones above and below the depth of the water table. NifH reads were translated with frameshift correction and 112,476 sequences were clustered at 5% amino acid dissimilarity resulting in 1,631 OTUs. Sample depth in relation to water table depth was correlated to differences in the NifH sequence classes. NifH sequences most closely related to group I nifH-harboring Alpha- and Beta Proteobacteria were in higher abundance above water table depth while those related to group III nifH-harboring Delta and Gamma Proteobacteria were more abundant below. The most dominant below water table depth NifH sequences, comprising 1/3 of the total, were distantly related to Verrucomicrobia-Opitutaceae. Overall, these results suggest that permafrost thaw alters the class-level composition of N2-fixing communities in the thawed soil layers and that this distinction corresponds to the depth of the water table. These nifH data were also compared to nifH sequences obtained from a study at an Alaskan taiga site, and to those of other geographically distant, non-permafrost sites. The two Alaska sites were differentiated largely by changes in relative abundances of the same OTUs, whereas the non-Alaska sites were differentiated by the lack of many Alaskan OTUs, and the presence of unique halophilic, sulfate- and iron-reducing taxa in the Alaska sites.

Ecological evaluation of proposed discharge of dredged material from Oakland Harbor into ocean waters (Phase 3 B of -42-foot project)

Energy Technology Data Exchange (ETDEWEB)

Kohn, N.P.; Ward, J.A.; Mayhew, H.L.; Word, J.Q.; Barrows, E.S.; Goodwin, S.M.; Lefkovitz, L.F. (Battelle/Marine Sciences Lab., Sequim, WA (United States))

1992-06-01

The Water Resources Development Act of 1986 (Public Law 99-662) authorized the US Army Corps of Engineers (USACE) San Francisco District, to deepen and widen the navigational channels of the Oakland Inner and Outer Harbors to accommodate deeper-draft vessels. The USACE is considering several disposal options for the dredged material removed during these channel improvements including open-water disposal. Dredged material proposed for open-water disposal must be evaluated to determine the potential impacts of the disposal activity on the water column and disposal site environments. The USACE requested that Battelle/Marine Sciences Laboratory (MSL) conduct studies to evaluate open-water disposal options for Oakland Harbor sediments. This request developed into the Oakland Harbor Phase III Program. This is Volume 2 of a two-volume report that presents information gathered to determine the suitability of ocean disposal of sediments dredged from Oakland Harbor. This volume contains the Appendixes (A through N), which provide details of the data analyses and full presentation of the data and results.

The Nucleosome Assembly Activity of NAP1 Is Enhanced by Alien▿

OpenAIRE

Eckey, Maren; Hong, Wei; Papaioannou, Maria; Baniahmad, Aria

2007-01-01

The assembly of nucleosomes into chromatin is essential for the compaction of DNA and inactivation of the DNA template to modulate and repress gene expression. The nucleosome assembly protein 1, NAP1, assembles nucleosomes independent of DNA synthesis and was shown to enhance coactivator-mediated gene expression, suggesting a role for NAP1 in transcriptional regulation. Here, we show that Alien, known to harbor characteristics of a corepressor of nuclear hormone receptors such as of the vitam...

78 FR 63136 - Drawbridge Operation Regulation; Inner Harbor Navigational Canal, New Orleans, LA

Science.gov (United States)

2013-10-23

...-AA09 Drawbridge Operation Regulation; Inner Harbor Navigational Canal, New Orleans, LA AGENCY: Coast... 4.6, both at New Orleans, LA. This proposed change would allow for the safe navigation of vessels... Department of Transportation and Development (LDOTD) (representing the New Orleans Levee District which is...

33 CFR 165.116 - Safety and Security Zones; Salem and Boston Harbors, Massachusetts.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Safety and Security Zones; Salem..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PORTS AND WATERWAYS SAFETY REGULATED NAVIGATION AREAS AND LIMITED... § 165.116 Safety and Security Zones; Salem and Boston Harbors, Massachusetts. (a) Location. The...

One surgeon's Army experience with "wound shock" from Pearl Harbor to the present.

Science.gov (United States)

Hardaway, Robert M

2009-09-01

The Army has had extensive experience in the study and treatment of shock, beginning with the American Civil War and continuing to the present. This is the story of one Army surgeon's experience, both in research and treatment of shock, from Pearl Harbor to the present.

76 FR 57087 - Stone Harbor Investment Partners LP, et al.; Notice of Application

Science.gov (United States)

2011-09-15

... SECURITIES AND EXCHANGE COMMISSION [Investment Company Act Release No. 29784; File No. 812-13931] Stone Harbor Investment Partners LP, et al.; Notice of Application September 7, 2011. AGENCY: Securities..., or cause more than 10% of the acquired company's voting stock to be owned by investment companies and...

78 FR 36662 - Safety Zone; Fairport Harbor Mardi Gras, Lake Erie, Fairport, OH

Science.gov (United States)

2013-06-19

... the Fairport Harbor Mardi Gras Fireworks display. This temporary safety zone is necessary to protect spectators and vessels from the hazards associated with a fireworks display. DATES: This rule is effective...: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on Lake Erie...

Bacterial Communities in Polluted Seabed Sediments: A Molecular Biology Assay in Leghorn Harbor

Directory of Open Access Journals (Sweden)

Carolina Chiellini

2013-01-01

Full Text Available Seabed sediments of commercial ports are often characterized by high pollution levels. Differences in number and distribution of bacteria in such areas can be related to distribution of pollutants in the port and to sediment conditions. In this study, the bacterial communities of five sites from Leghorn Harbor seabed were characterized, and the main bacterial groups were identified. T-RFLP was used for all samples; two 16S rRNA libraries and in silico digestion of clones were used to identify fingerprint profiles. Library data, phylogenetic analysis, and T-RFLP coupled with in silico digestion of the obtained sequences evidenced the dominance of Proteobacteria and the high percentage of Bacteroidetes in all sites. The approach highlighted similar bacterial communities between samples coming from the five sites, suggesting a modest differentiation among bacterial communities of different harbor seabed sediments and hence the capacity of bacterial communities to adapt to different levels and types of pollution.

Analysis and Enhancements of a Prolific Macroscopic Model of Epilepsy

Directory of Open Access Journals (Sweden)

Christopher Fietkiewicz

2016-01-01

Full Text Available Macroscopic models of epilepsy can deliver surprisingly realistic EEG simulations. In the present study, a prolific series of models is evaluated with regard to theoretical and computational concerns, and enhancements are developed. Specifically, we analyze three aspects of the models: (1 Using dynamical systems analysis, we demonstrate and explain the presence of direct current potentials in the simulated EEG that were previously undocumented. (2 We explain how the system was not ideally formulated for numerical integration of stochastic differential equations. A reformulated system is developed to support proper methodology. (3 We explain an unreported contradiction in the published model specification regarding the use of a mathematical reduction method. We then use the method to reduce the number of equations and further improve the computational efficiency. The intent of our critique is to enhance the evolution of macroscopic modeling of epilepsy and assist others who wish to explore this exciting class of models further.

Psychosocial Pain Management Moderation: The Limit, Activate, and Enhance Model.

Science.gov (United States)

Day, Melissa A; Ehde, Dawn M; Jensen, Mark P

2015-10-01

There is a growing emphasis in the pain literature on understanding the following second-order research questions: Why do psychosocial pain treatments work? For whom do various treatments work? This critical review summarizes research that addresses the latter question and proposes a moderation model to help guide future research. A theoretical moderation framework for matching individuals to specific psychosocial pain interventions has been lacking. However, several such frameworks have been proposed in the broad psychotherapy and implementation science literature. Drawing on these theories and adapting them specifically for psychosocial pain treatment, here we propose a Limit, Activate, and Enhance model of pain treatment moderation. This model is unique in that it includes algorithms not only for matching treatments on the basis of patient weaknesses but also for directing patients to interventions that build on their strengths. Critically, this model provides a basis for specific a priori hypothesis generation, and a selection of the possible hypotheses drawn from the model are proposed and discussed. Future research considerations are presented that could refine and expand the model based on theoretically driven empirical evidence. The Limit, Activate, and Enhance model presented here is a theoretically derived framework that provides an a priori basis for hypothesis generation regarding psychosocial pain treatment moderators. The model will advance moderation research via its unique focus on matching patients to specific treatments that (1) limit maladaptive responses, (2) activate adaptive responses, and (3) enhance treatment outcomes based on patient strengths and resources. Copyright © 2015 American Pain Society. Published by Elsevier Inc. All rights reserved.

Grace Under Fire: The Army Nurses of Pearl Harbor, 1941.

Science.gov (United States)

Milbrath, Gwyneth R

2016-01-01

Much has been written about the military events of December 7, 1941; however, little has been documented about the nurses' work and experience at Pearl Harbor, Hawaii. The aerial assault on Pearl Harbor was the first time in US history that Army nurses had been on the front line of battle. Nurses quickly triaged and stabilized those who could be saved, and provided compassion and comfort to those who were dying, in an environment where the nurses were unsure of their own survival. Traditional historical methods and a social history framework were used in this investigation. Primary sources included oral histories from the US Army Medical Department Center of History and Heritage and the State of Hawaii's website, Hawaii Aviation. Secondary sources included published books, newspaper articles, military websites, and history texts. Due to the limited bed capacity, Hickam Field Hospital converted to an evacuation hospital. Nurses, physicians, and medical corpsman triaged, stabilized, and transported those likely to survive, while staging the dead behind the building. The emergency room at Tripler Hospital was quickly flooded with patients from the battlefield, but the staff was able to sort patients appropriately to the wards, to the operating room, or provide comfort care as they died. At Schofield Hospital, collaboration between tireless doctors, nurses, and corpsmen was key to providing life-saving surgery and care.

DEPOT MAINTENANCE: Key Financial Issues for Consolidations at Pearl Harbor and Elsewhere Are Still Unresolved

National Research Council Canada - National Science Library

2001-01-01

.... In September 1999, we reported that the preliminary results of the ongoing Pearl Harbor pilot were mixed and recommended that the Departments of Defense and the Navy address unresolved issues related...

Synanthropic Cockroaches (Blattidae: Periplaneta spp.) Harbor Pathogenic Leptospira in Colombia.

Science.gov (United States)

Gonzalez-Astudillo, Viviana; Bustamante-Rengifo, Javier A; Bonilla, Álvaro; Lehmicke, Anna Joy J; Castillo, Andrés; Astudillo-Hernández, Miryam

2016-01-01

Leptospirosis cases in Colombia are typically linked to peridomestic rodents; however, empirical data suggest that Leptospira-infected patients with no apparent exposure to these reservoirs are common. Cockroaches (Periplaneta spp.) have equal or greater interaction with humans than rodents, yet their potential role as carriers of Leptospira has not been assessed. We determined if pathogenic Leptospira is harbored by Periplaneta spp. in Cali (Colombia) and the variables influencing this relationship. Fifty-nine cockroaches were captured from seven sites and DNA was extracted from the body surface and digestive tract for a multiplex polymerase chain reaction, targeting genes secY and flaB. Logistic regression models and proportion tests showed a higher likelihood for Leptospira to be isolated from body surfaces (P > 0.001) and from individuals inside houses (six times more likely). These findings are the first to demonstrate an association between Periplaneta spp. and Leptospira, suggesting the need to investigate the potential for cockroaches to serve as reservoirs or transport hosts for Leptospira. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

78 FR 27304 - Safety Zone; Melrose Pyrotechnics Fireworks Display; Chicago Harbor, Chicago, IL

Science.gov (United States)

2013-05-10

... environmental risk to health or risk to safety that may disproportionately affect children. 11. Indian Tribal... Pier in Chicago Harbor, Chicago, IL. The Captain of the Port, Lake Michigan, has determined that these fireworks displays will pose a significant risk to public safety and property. Such hazards include falling...

Competence Models in Technology-enhanced Competence-based Learning

NARCIS (Netherlands)

Sampson, Demetrios; Fytros, Demetrios

2008-01-01

Please cite as: Sampson, D., & Fytros, D. (2008). Competence Models in Technology-enhanced Competence-based Learning. In H. H. Adelsberger, Kinshuk, J. M. Pawlowski & D. Sampson (Eds.), International Handbook on Information Technologies for Education and Training, 2nd Edition, Springer, June 2008

Brain Meta-Transcriptomics from Harbor Seals to Infer the Role of the Microbiome and Virome in a Stranding Event.

Science.gov (United States)

Rosales, Stephanie M; Thurber, Rebecca Vega

2015-01-01

Marine diseases are becoming more frequent, and tools for identifying pathogens and disease reservoirs are needed to help prevent and mitigate epizootics. Meta-transcriptomics provides insights into disease etiology by cataloguing and comparing sequences from suspected pathogens. This method is a powerful approach to simultaneously evaluate both the viral and bacterial communities, but few studies have applied this technique in marine systems. In 2009 seven harbor seals, Phoca vitulina, stranded along the California coast from a similar brain disease of unknown cause of death (UCD). We evaluated the differences between the virome and microbiome of UCDs and harbor seals with known causes of death. Here we determined that UCD stranded animals had no viruses in their brain tissue. However, in the bacterial community, we identified Burkholderia and Coxiella burnetii as important pathogens associated with this stranding event. Burkholderia were 100% prevalent and ~2.8 log2 fold more abundant in the UCD animals. Further, while C. burnetii was found in only 35.7% of all samples, it was highly abundant (~94% of the total microbial community) in a single individual. In this harbor seal, C. burnetii showed high transcription rates of invading and translation genes, implicating it in the pathogenesis of this animal. Based on these data we propose that Burkholderia taxa and C. burnetii are potentially important opportunistic neurotropic pathogens in UCD stranded harbor seals.

Brain Meta-Transcriptomics from Harbor Seals to Infer the Role of the Microbiome and Virome in a Stranding Event.

Directory of Open Access Journals (Sweden)

Stephanie M Rosales

Full Text Available Marine diseases are becoming more frequent, and tools for identifying pathogens and disease reservoirs are needed to help prevent and mitigate epizootics. Meta-transcriptomics provides insights into disease etiology by cataloguing and comparing sequences from suspected pathogens. This method is a powerful approach to simultaneously evaluate both the viral and bacterial communities, but few studies have applied this technique in marine systems. In 2009 seven harbor seals, Phoca vitulina, stranded along the California coast from a similar brain disease of unknown cause of death (UCD. We evaluated the differences between the virome and microbiome of UCDs and harbor seals with known causes of death. Here we determined that UCD stranded animals had no viruses in their brain tissue. However, in the bacterial community, we identified Burkholderia and Coxiella burnetii as important pathogens associated with this stranding event. Burkholderia were 100% prevalent and ~2.8 log2 fold more abundant in the UCD animals. Further, while C. burnetii was found in only 35.7% of all samples, it was highly abundant (~94% of the total microbial community in a single individual. In this harbor seal, C. burnetii showed high transcription rates of invading and translation genes, implicating it in the pathogenesis of this animal. Based on these data we propose that Burkholderia taxa and C. burnetii are potentially important opportunistic neurotropic pathogens in UCD stranded harbor seals.

78 FR 62439 - Drawbridge Operation Regulation; Inner Harbor Navigational Canal, New Orleans, LA

Science.gov (United States)

2013-10-22

... Operation Regulation; Inner Harbor Navigational Canal, New Orleans, LA AGENCY: Coast Guard, DHS. ACTION..../Seabrook) bridge across the IHNC, mile 4.6, both at New Orleans, LA. This deviation will test changes to... Intra-Costal Waterway (GIWW). The US 90 (Danzinger) Bridge across the IHNC, mile 3.1, at New Orleans...

75 FR 8486 - Drawbridge Operation Regulation; Inner Harbor Navigational Canal, New Orleans, LA

Science.gov (United States)

2010-02-25

... Operation Regulation; Inner Harbor Navigational Canal, New Orleans, LA AGENCY: Coast Guard, DHS. ACTION... EHL), at New Orleans, LA. The deviation is necessary to replace the counterweight wire ropes on the... Building Ground Floor, Room W12-140, 1200 New Jersey Avenue, SE., Washington, DC 20590, between 9 a.m. and...

Aerial Survey Counts of Harbor Seals in Lake Iliamna, Alaska, 1984-2013 (NODC Accession 0123188)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This dataset provides counts of harbor seals from aerial surveys over Lake Iliamna, Alaska, USA. The data have been collated from three previously published sources...

A Dataset of Aerial Survey Counts of Harbor Seals in Iliamna Lake, Alaska: 1984-2013

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — This dataset provides counts of harbor seals from aerial surveys over Iliamna Lake, Alaska, USA. The data have been collated from three previously published sources...

Characteristics and management of Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemase in a tertiary hospital.

Science.gov (United States)

Pang, Feng; Jia, Xiu-Qin; Song, Zhen-Zhu; Li, Yan-Hua; Wang, Bin; Zhao, Qi-Gang; Wang, Chuan-Xin; Zhang, Yi; Wang, Le-Xin

2016-03-01

The emergence of Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemases is rare. We report an occurrence of Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemases in a Chinese tertiary care hospital from November 2010 to December 2012. The clinical characteristics of 30 patients were described. The genetic relationship of isolates was determined by pulsed-field gel electrophoresis (PFGE). Carbapenemases were detected by modified Hodge test (MHT) and polymerase chain reactions (PCRs). Amplicons were sequenced and blasted to determine the genotype. Most infected patients were from intensive care unit and had complex and serious underlying illnesses requiring mechanical ventilation. PFGE revealed that Klebsiella pneumoniae showed two major PFGE types. Two Klebsiella oxytoca had an indistinguishable PFGE pattern, while four Enterobacter cloacae were different strains. The sequencing studies showed Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemase in the 23 infected patients. The majority of patients had infections with the carbapenemase-producing Enterobacteriaceae (CPE) strain, most were successfully treated with a range of antibiotics and discharged. It is important to maintain a high index of suspicion to screen for carbapenemase-producing Enterobacteriaceae strains. Rapid identification of these strains and implementation of stringent procedures are the key to prevent major outbreaks in a hospital setting.

Endoscopic tattooing of early colon carcinoma enhances detection of lymph nodes most prone to harbor tumor burden.

Science.gov (United States)

Aldecoa, Iban; Montironi, Carla; Planell, Nuria; Pellise, Maria; Fernandez-Esparrach, Gloria; Gines, Angels; Delgado, Salvadora; Momblan, Dulce; Moreira, Leticia; Lopez-Ceron, Maria; Rakislova, Natalia; Martinez-Palli, Graciela; Balust, Jaume; Bombi, Josep Antoni; de Lacy, Antonio; Castells, Antoni; Balaguer, Francesc; Cuatrecasas, Miriam

2017-02-01

Colorectal cancer (CRC) screening programs result in the detection of early-stage asymptomatic carcinomas suitable to be surgically cured. Lymph nodes (LN) from early CRC are usually small and may be difficult to collect. Still, at least 12 LNs should be analyzed from colectomies, to ensure a reliable pN0 stage. Presurgical endoscopic tattooing improves LN procurement. In addition, molecular detection of occult LN tumor burden in histologically pN0 CRC patients is associated with a decreased survival rate. We aimed to study the impact of presurgical endoscopic tattooing on the molecular detection of LN tumor burden in early colon neoplasms. A prospective cohort study from a CRC screening-based population was performed at a tertiary academic hospital. LNs from colectomies with and without preoperative endoscopic tattooing were assessed by two methods, hematoxylin and eosin (HE), and RT-LAMP, to detect tumor cytokeratin 19 (CK19) mRNA. We compared the amount of tumor burden and LN yields from tattooed and non-tattooed specimens. HE and RT-LAMP analyses of 936 LNs were performed from 71 colectomies containing early carcinomas and endoscopically unresectable adenomas (8 pT0, 17 pTis, 27 pT1, 19 pT2); 47 out of 71 (66.2 %) were tattooed. Molecular positivity correlated with the presence of tattoo in LN [p < 0.001; OR 3.1 (95 % CI 1.7-5.5)]. A significantly higher number of LNs were obtained in tattooed specimens (median 17 LN vs. 14.5 LN; p = 0.019). Endoscopic tattooing enables the analysis of those LNs most prone to harbor tumor cells and improves the number of LN harvested.

Enhanced phytoremediation in the vadose zone: Modeling and column studies

Science.gov (United States)

Sung, K.; Chang, Y.; Corapcioglu, M.; Cho, C.

2002-05-01

Phytoremediation is a plant-based technique with potential for enhancing the remediation of vadoese zone soils contaminated by pollutants. The use of deep-rooted plants is an alternative to conventional methodologies. However, when the phytoremediation is applied to the vadose zone, it might have some restrictions since it uses solely naturally driven energy and mechanisms in addition to the complesxity of the vadose zone. As a more innovative technique than conventional phytoremediation methods, air injected phytoremediation technique is introduced to enhance the remediation efficiency or to apply at the former soil vapor extraction or bio venting sites. Effects of air injection, vegetation treatment, and air injection with vegetation treatments on the removal of hydrocarbon were investigated by column studies to simulate the field situation. Both the removal efficiency and the microbial activity were highest in air-injected and vegetated column soils. It was suggested that increased microorganisms activity stimulated by plant root exudates enhanced biodegradation of hydrocarbon compounds. Air injection provided sufficient opportunity for promoting the microbial activity at depths where the conditions are anaerobic. Air injection can enhance the physicochemical properties of the medium and contaminant and increase the bioavailability i.e., the plant and microbial accessibility to the contaminant. A mathematical model that can be applied to phytoremediation, especially to air injected phytoremediation, for simulating the fate and the transport of a diesel contaminant in the vadose zone is developed. The approach includes a two-phase model of water flow in vegetated and unplanted vadose zone soil. A time-specific root distribution model and a microbial growth model in the rhizosphere of vegetated soil were combined with an unsaturated soil water flow equation as well as with a contaminant transport equation. The proposed model showed a satisfactory representation of

Assay dependence of Brucella antibody prevalence in a declining Alaskan harbor seal (Phoca vitulina population

Directory of Open Access Journals (Sweden)

Hueffer Karsten

2013-01-01

Full Text Available Abstract Background Brucella is a group of bacteria that causes brucellosis, which can affect population health and reproductive success in many marine mammals. We investigated the serological prevalence of antibodies against Brucella bacteria in a declining harbor seal population in Glacier Bay National Park, Alaska. Results Prevalence ranged from 16 to 74 percent for those tests detecting antibodies, indicating that harbor seals in Glacier Bay have been exposed to Brucella bacteria. However, the actual level of serological prevalence could not be determined because results were strongly assay-dependent. Conclusions This study reinforces the need to carefully consider assay choice when comparing different studies on the prevalence of anti–Brucella antibodies in pinnipeds and further highlights the need for species- or taxon-specific assay validation for both pathogen and host species.

75 FR 18887 - Division of Longshore and Harbor Workers' Compensation; Proposed Collection; Comment Request

Science.gov (United States)

2010-04-13

... can be provided in the desired format, reporting burden (time and financial resources) is minimized... comment on proposed and/or continuing collections of information in accordance with the paperwork... concerning the proposed collection: Securing Financial Obligations under the Longshore and Harbor Workers...

Detection of multidrug-resistant Pseudomonas aeruginosa harboring bla GES-1 and bla GES-11 in Recife, Brazil

Directory of Open Access Journals (Sweden)

Valdemir Vicente da Silva Júnior

Full Text Available Abstract INTRODUCTION: Pseudomonas aeruginosa, an important pathogen globally, presents several resistance mechanisms. This study aimed to investigate the presence of bla GES in clinical isolates of Pseudomonas aeruginosa obtained from various clinical specimens from patients admitted to three different hospitals in Recife, Brazil. The Guiana extended spectrum beta-lactamase (GES enzymes are responsible for conferring broad spectrum resistance to beta-lactam drugs, including the carbapenems. METHODS: A total of 100 carbapenem-resistant P. aeruginosa isolates underwent polymerase chain reaction (PCR testing to identify bla GES, bla KPC, bla SPM-1, bla IMP, and bla VIM. Additionally, PCR products positive for bla GES were sequenced. The clonal profiles of these same isolates were then determined by means of enterobacterial repetitive intergenic consensus (ERIC-PCR analysis. RESULTS: PCR analysis revealed that four isolates harbored bla GES; DNA sequencing showed that two harbored bla GES-1 and two bla GES-11. Beta-lactamase genes bla SPM-1, bla IMP, bla VIM, and bla KPC were investigated; none of these genes was detected. Automated susceptibility testing methods (Vitek®2, bioMérieux showed that the bla GES-1-positive isolates were only susceptible to polymyxin B. The patterns obtained with ERIC-PCR methods showed clonal relationship between the two isolates that harbored bla GES-11, whereas different clonal profiles were found in the isolates harboring bla GES-1. CONCLUSIONS: We detected the presence of bacterial isolates positive for two different variants of the enzyme GES in three different hospitals from Recife, Brazil. These enzymes have a great capacity for dissemination among Gram-negative bacteria and confer broad-spectrum resistance to beta-lactam antibiotics and to the carbapenems.

The model of illumination-transillumination for image enhancement of X-ray images

Energy Technology Data Exchange (ETDEWEB)

Lyu, Kwang Yeul [Shingu College, Sungnam (Korea, Republic of); Rhee, Sang Min [Kangwon National Univ., Chuncheon (Korea, Republic of)

2001-06-01

In digital image processing, the homomorphic filtering approach is derived from an illumination - reflectance model of the image. It can also be used with an illumination-transillumination model X-ray film. Several X-ray images were applied to enhancement with histogram equalization and homomorphic filter based on an illumination-transillumination model. The homomorphic filter has proven theoretical claim of image density range compression and balanced contrast enhancement, and also was found a valuable tool to process analog X-ray images to digital images.

CHIRP survey of the submerged harbors of King Herod's Caesarea, offshore Israel - looking for evidence of ancient disasters

Science.gov (United States)

Austin, J. A.; Goodman-Tchernov, B.

2012-12-01

Caesarea Maritima, located on the Israel coast south of Haifa, is an ancient city/harbor site built ~2,000 years ago that continues to produce important insights into both the maritime livelihoods of antiquity and natural/anthropogenic influences on coastal structures/processes. The grandiose, three-basin harbor complex was built by Herod the Great. Today, the inner harbor basin is terrestrial, while the majority of the intermediate/outer harbors lies 1-7 m beneath the sea surface. Explanations for the harbor's destruction have included earthquakes, liquefaction, deterioration from erosion, storm undercutting and tsunami damage. Recently, evidence for tsunami events has been discovered offshore during excavation and coring expeditions, specifically trench exposures and pneumatic hammer cores. Stratigraphic horizons containing tsunamite signatures suggest the occurrence of at least three tsunamis in the past ~3,500 yrs. One may correspond with the catastrophic eruption of Santorini ~1500 BC; another appears to have done significant damage to Caesarea ~115 AD, and a third occurred within the period 5th-7th centuries AD. Extant combined bathymetric/magnetic surveys offshore also show unusual, anomalous features. Excavations in 2003 suggest that these features may be part of an ancient designated anchoring station, and support the existence of underlying man-made terrestrial structures from an earlier period when sea-level was lower. The primary goal of the current survey was to correlate recognized/potential tsunamigenic sediment layers throughout the proximal shelf offshore Caesarea, using extremely high-resolution geophysical images. Such surficial sub-bottom profiling will allow archaeologists to proceed with investigations of recognized anthropogenically-influenced submerged coastal features. The field survey was conducted in August 2011. Seismic data were collected using UTIG's portable Knudsen 320BP CHIRP (2.5-5.5 kHz) profiler, affixed to a metal pole mounted

Enhancing Architectural Drawings and Models with Photoshop

CERN Document Server

Onstott, Scott

2010-01-01

Transform your CAD drawings into powerful presentationThis one-of-a-kind book shows you how to use Photoshop to turn CAD drawings and BIM models into artistic presentations with captivating animations, videos, and dynamic 3D imagery. The techniques apply to all leading architectural design software including AutoCAD, Revit, and 3ds Max Design. Video tutorials on the DVD improve your learning curve and let you compare your work with the author's.Turn CAD drawings and BIM models into powerful presentations featuring animation, videos, and 3D imagery for enhanced client appealCraft interactive pa

An enhanced fire hazard assessment model and validation experiments for vertical cable trays

International Nuclear Information System (INIS)

Li, Lu; Huang, Xianjia; Bi, Kun; Liu, Xiaoshuang

2016-01-01

Highlights: • An enhanced model was developed for vertical cable fire hazard assessment in NPP. • The validated experiments on vertical cable tray fires were conducted. • The capability of the model for cable tray with different cable spacing were tested. - Abstract: The model, referred to as FLASH-CAT (Flame Spread over Horizontal Cable Trays), was developed to estimate the heat release rate for vertical cable tray fire. The focus of this work is to investigate the application of an enhanced model to the single vertical cable tray fires with different cable spacing. The experiments on vertical cable tray fires with three typical cable spacing were conducted. The histories of mass loss rate and flame length were recorded during the cable fire. From the experimental results, it is found that the space between cable lines intensifies the cable combustion and accelerates the flame spread. The predictions by the enhanced model show good agreements with the experimental data. At the same time, it is shown that the enhanced model is capable of predicting the different behaviors of cable fires with different cable spacing by adjusting the flame spread speed only.

An enhanced fire hazard assessment model and validation experiments for vertical cable trays

Energy Technology Data Exchange (ETDEWEB)

Li, Lu [Sate Key Laboratory of Fire Science, University of Science and Technology of China, Hefei 230027 (China); Huang, Xianjia, E-mail: huangxianjia@gziit.ac.cn [Joint Laboratory of Fire Safety in Nuclear Power Plants, Institute of Industry Technology Guangzhou & Chinese Academy of Sciences, Guangzhou 511458 (China); Bi, Kun; Liu, Xiaoshuang [China Nuclear Power Design Co., Ltd., Shenzhen 518045 (China)

2016-05-15

Highlights: • An enhanced model was developed for vertical cable fire hazard assessment in NPP. • The validated experiments on vertical cable tray fires were conducted. • The capability of the model for cable tray with different cable spacing were tested. - Abstract: The model, referred to as FLASH-CAT (Flame Spread over Horizontal Cable Trays), was developed to estimate the heat release rate for vertical cable tray fire. The focus of this work is to investigate the application of an enhanced model to the single vertical cable tray fires with different cable spacing. The experiments on vertical cable tray fires with three typical cable spacing were conducted. The histories of mass loss rate and flame length were recorded during the cable fire. From the experimental results, it is found that the space between cable lines intensifies the cable combustion and accelerates the flame spread. The predictions by the enhanced model show good agreements with the experimental data. At the same time, it is shown that the enhanced model is capable of predicting the different behaviors of cable fires with different cable spacing by adjusting the flame spread speed only.

33 CFR 334.470 - Cooper River and Charleston Harbor, S.C.; restricted areas.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Cooper River and Charleston....470 Cooper River and Charleston Harbor, S.C.; restricted areas. (a) The restricted areas. (1) Area No. 1 is that portion of the Cooper River beginning near the westerly shore north of Shipyard Creek at...

Radiological survey of the Pearl Harbor Naval Shipyard and Environs, Honolulu, Hawaii. Final report

International Nuclear Information System (INIS)

Callis, R.S.

1987-06-01

This report presents results of the survey conducted by the Eastern Environmental Radiation Facility personnel to assess levels of environmental radioactivity resulting from maintenance and operation of nuclear-powered warships at the Pearl Harbor Naval Shipyard and Environs on the Hawaiian Island of Oahu

Enhancing Students' Communication Skills through Treffinger Teaching Model

Science.gov (United States)

Alhaddad, Idrus; Kusumah, Yaya S.; Sabandar, Jozua; Dahlan, Jarnawi A.

2015-01-01

This research aims to investigate, compare, and describe the achievement and enhancement of students' mathematical communication skills (MCS). It based on the prior mathematical knowledge (PMK) category (high, medium and low) by using Treffinger models (TM) and conventional learning (CL). This research is an experimental study with the population…

Enhanced pairing susceptibility in a photodoped two-orbital Hubbard model

Science.gov (United States)

Werner, Philipp; Strand, Hugo U. R.; Hoshino, Shintaro; Murakami, Yuta; Eckstein, Martin

2018-04-01

Local spin fluctuations provide the glue for orbital-singlet spin-triplet pairing in the doped Mott insulating regime of multiorbital Hubbard models. At large Hubbard repulsion U , the pairing susceptibility is nevertheless tiny because the pairing interaction cannot overcome the suppression of charge fluctuations. Using nonequilibrium dynamical mean field simulations of the two-orbital Hubbard model, we show that out of equilibrium the pairing susceptibility in this large-U regime can be strongly enhanced by creating a photoinduced population of the relevant charge states. This enhancement is supported by the long lifetime of photodoped charge carriers and a built-in cooling mechanism in multiorbital Hubbard systems.

76 FR 82117 - Regulations Implementing the Longshore and Harbor Workers' Compensation Act: Recreational Vessels

Science.gov (United States)

2011-12-30

... activity, and whether it falls within the Coast Guard definition of a non- recreational vessel less than 20... recreational vessel work, the employee will be eligible for benefits based on the covered work. The last... Regulations Implementing the Longshore and Harbor Workers' Compensation Act: Recreational Vessels AGENCY...

78 FR 57319 - Children's Online Privacy Protection Rule Safe Harbor Proposed Self-Regulatory Guidelines...

Science.gov (United States)

2013-09-18

...-AB20 Children's Online Privacy Protection Rule Safe Harbor Proposed Self-Regulatory Guidelines; kidSAFE... proposed self-regulatory guidelines submitted by the kidSAFE Seal Program (``kidSAFE''), owned and operated... enabling industry groups or others to submit to the Commission for approval self-regulatory guidelines that...

Feeding kinematics, suction, and hydraulic jetting performance of harbor seals (Phoca vitulina.

Directory of Open Access Journals (Sweden)

Christopher D Marshall

Full Text Available The feeding kinematics, suction and hydraulic jetting capabilities of captive harbor seals (Phoca vitulina were characterized during controlled feeding trials. Feeding trials were conducted using a feeding apparatus that allowed a choice between biting and suction, but also presented food that could be ingested only by suction. Subambient pressure exerted during suction feeding behaviors was directly measured using pressure transducers. The mean feeding cycle duration for suction-feeding events was significantly shorter (0.15±0.09 s; P<0.01 than biting feeding events (0.18±0.08 s. Subjects feeding in-water used both a suction and a biting feeding mode. Suction was the favored feeding mode (84% of all feeding events compared to biting, but biting comprised 16% of feeding events. In addition, seals occasionally alternated suction with hydraulic jetting, or used hydraulic jetting independently, to remove fish from the apparatus. Suction and biting feeding modes were kinematically distinct regardless of feeding location (in-water vs. on-land. Suction was characterized by a significantly smaller gape (1.3±0.23 cm; P<0.001 and gape angle (12.9±2.02°, pursing of the rostral lips to form a circular aperture, and pursing of the lateral lips to occlude lateral gape. Biting was characterized by a large gape (3.63±0.21 cm and gape angle (28.8±1.80°; P<0.001 and lip curling to expose teeth. The maximum subambient pressure recorded was 48.8 kPa. In addition, harbor seals were able to jet water at food items using suprambient pressure, also known as hydraulic jetting. The maximum hydraulic jetting force recorded was 53.9 kPa. Suction and hydraulic jetting where employed 90.5% and 9.5%, respectively, during underwater feeding events. Harbor seals displayed a wide repertoire of behaviorally flexible feeding strategies to ingest fish from the feeding apparatus. Such flexibility of feeding strategies and biomechanics likely forms the basis of their

Formal safety assessment based on relative risks model in ship navigation

Energy Technology Data Exchange (ETDEWEB)

Hu Shenping [Merchant Marine College, Shanghai Maritime University, 1550, Pudong Dadao, Shanghai 200135 (China)]. E-mail: sphu@mmc.shmtu.edu.cn; Fang Quangen [Merchant Marine College, Shanghai Maritime University, 1550, Pudong Dadao, Shanghai 200135 (China)]. E-mail: qgfang@mmc.shmtu.edu.cn; Xia Haibo [Merchant Marine College, Shanghai Maritime University, 1550, Pudong Dadao, Shanghai 200135 (China)]. E-mail: hbxia@mmc.shmtu.edu.cn; Xi Yongtao [Merchant Marine College, Shanghai Maritime University, 1550, Pudong Dadao, Shanghai 200135 (China)]. E-mail: xiyt@mmc.shmtu.edu.cn

2007-03-15

Formal safety assessment (FSA) is a structured and systematic methodology aiming at enhancing maritime safety. It has been gradually and broadly used in the shipping industry nowadays around the world. On the basis of analysis and conclusion of FSA approach, this paper discusses quantitative risk assessment and generic risk model in FSA, especially frequency and severity criteria in ship navigation. Then it puts forward a new model based on relative risk assessment (MRRA). The model presents a risk-assessment approach based on fuzzy functions and takes five factors into account, including detailed information about accident characteristics. It has already been used for the assessment of pilotage safety in Shanghai harbor, China. Consequently, it can be proved that MRRA is a useful method to solve the problems in the risk assessment of ship navigation safety in practice.

Formal safety assessment based on relative risks model in ship navigation

International Nuclear Information System (INIS)

Hu Shenping; Fang Quangen; Xia Haibo; Xi Yongtao

2007-01-01

Formal safety assessment (FSA) is a structured and systematic methodology aiming at enhancing maritime safety. It has been gradually and broadly used in the shipping industry nowadays around the world. On the basis of analysis and conclusion of FSA approach, this paper discusses quantitative risk assessment and generic risk model in FSA, especially frequency and severity criteria in ship navigation. Then it puts forward a new model based on relative risk assessment (MRRA). The model presents a risk-assessment approach based on fuzzy functions and takes five factors into account, including detailed information about accident characteristics. It has already been used for the assessment of pilotage safety in Shanghai harbor, China. Consequently, it can be proved that MRRA is a useful method to solve the problems in the risk assessment of ship navigation safety in practice

On the Influence of Inharmonicities in Model-Based Speech Enhancement

DEFF Research Database (Denmark)

Nørholm, Sidsel Marie; Jensen, Jesper Rindom; Christensen, Mads Græsbøll

2013-01-01

In relation to speech enhancement, we study the influence of modifying the harmonic signal model for voiced speech to include small perturbations in the frequencies of the harmonics. A perturbed signal model is incorporated in the nonlinear least squares method, the Capon filter and the amplitude...

Benthic grab data from October 1994 in the Lower Bay of New York/New Jersey Harbor

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Benthic grab samples were collected using a Shipek grab at pre-determined locations in the harbor. Taxonomic enumerations and biological community parameters were...

Design and execution of double slits type straight caisson at exclusive harbors of Ooma nuclear power plant

International Nuclear Information System (INIS)

Tsukamoto, Kunihiro; Yamamoto, Morikuni; Naruoka, Masayoshi; Matsubara, Takayuki

2004-01-01

Ooma nuclear power plant is going to build the exclusive harbors, of which west breakwater is a double slits type straight caisson bank (double slits caisson). It contains double transmission walls and retarding rooms, which show large effect of breaker, because the reflected wave height of short-period component is decreased in the rear retarding room and one of long-period component decreased in both retarding rooms. Accordingly, it is seemed to be proved larger effect of breaker than one slit type caisson. Outline of harbors construction, adoption of double slits caisson, design of caisson, construction of caisson and installation are stated. The effect of breaker of double slits caisson is shown. (S.Y.)

An enhanced tokamak startup model

Science.gov (United States)

Goswami, Rajiv; Artaud, Jean-François

2017-01-01

The startup of tokamaks has been examined in the past in varying degree of detail. This phase typically involves the burnthrough of impurities and the subsequent rampup of plasma current. A zero-dimensional (0D) model is most widely used where the time evolution of volume averaged quantities determines the detailed balance between the input and loss of particle and power. But, being a 0D setup, these studies do not take into consideration the co-evolution of plasma size and shape, and instead assume an unchanging minor and major radius. However, it is known that the plasma position and its minor radius can change appreciably as the plasma evolves in time to fill in the entire available volume. In this paper, an enhanced model for the tokamak startup is introduced, which for the first time takes into account the evolution of plasma geometry during this brief but highly dynamic period by including realistic one-dimensional (1D) effects within the broad 0D framework. In addition the effect of runaway electrons (REs) has also been incorporated. The paper demonstrates that the inclusion of plasma cross section evolution in conjunction with REs plays an important role in the formation and development of tokamak startup. The model is benchmarked against experimental results from ADITYA tokamak.

Strategies for Enhancing Nonlinear Internal Model Control of pH Processes

Energy Technology Data Exchange (ETDEWEB)

Hu, Qiuping.; Rangaiah, G.P. [The National University of Singapore, Singapore (Singapore). Dept. of Chemical and Environmental Engineering

1999-02-01

Control of neutralization processes is very difficult due to nonlinear dynamics, different types of disturbances and modeling errors. The objective of the paper is to evaluate two strategies (augmented internal model control, AuIMC and adaptive internal model control, AdIMC) for enhancing pH control by nonlinear internal model control (NIMC). A NIMC controller is derived directly form input output linearization. The AuIMC is composed of NIMC and an additional loop through which the difference between the process and model outputs is fed back and added to the input of the controller. For the AdIMC, and adaptive law with two tuning parameters is proposed for estimating the unknown parameter. Both AuIMC and AdIMC are extensively tested via simulation for pH neutralization. The theoretical and simulation results show that both the proposed strategies can reduce the effect of modeling errors and disturbances, and thereby enhance the performance of NIMC for pH processes. (author)

Processes influencing the transport and fate of contaminated sediments in the coastal ocean: Boston Harbor and Massachusetts Bay

Science.gov (United States)

Alexander, P. Soupy; Baldwin, Sandra M.; Blackwood, Dann S.; Borden, Jonathan; Casso, Michael A.; Crusius, John; Goudreau, Joanne; Kalnejais, Linda H.; Lamothe, Paul J.; Martin, William R.; Martini, Marinna A.; Rendigs, Richard R.; Sayles, Frederick L.; Signell, Richard P.; Valentine, Page C.; Warner, John C.; Bothner, Michael H.; Butman, Bradford

2007-01-01

Most of the major urban centers of the United States including Boston, New York, Washington, Chicago, New Orleans, Miami, Los Angeles, San Francisco, and Seattle—are on a coast (fig. 1.1). All of these cities discharge treated sewage effluent into adjacent waters. In 2000, 74 percent of the U.S. population lived within 200 kilometers (km) of the coast. Between 1980 and 2002, the population density in coastal communities increased approximately 4.5 times faster than in noncoastal areas of the U.S. (Perkins, 2004). More people generate larger volumes of wastes, increase the demands on wastewater treatment, expand the area of impervious land surfaces, and use more vehicles that contribute contaminants to street runoff. According to the National Coastal Condition Report II (U.S. Environmental Protection Agency, 2005a), on the basis of coastal habitat, water and sediment quality, benthic index, and fish tissue, the overall national coastal condition is only poor to fair and the overall coastal condition in the highly populated Northeast is poor. Scientific information helps managers to prioritize and regulate coastal-ocean uses that include recreation, commercial fishing, transportation, waste disposal, and critical habitat for marine organisms. These uses are often in conflict with each other and with environmental concerns. Developing a strategy for managing competing uses while maintaining sustainability of coastal resources requires scientific understanding of how the coastal ocean system behaves and how it responds to anthropogenic influences. This report provides a summary of a multidisciplinary research program designed to improve our understanding of the transport and fate of contaminants in Massachusetts coastal waters. Massachusetts Bay and Boston Harbor have been a focus of U.S. Geological Survey (USGS) research because they provide a diverse geographic setting for developing a scientific understanding of the geology, geochemistry, and oceanography of

Enhanced pid vs model predictive control applied to bldc motor

Science.gov (United States)

Gaya, M. S.; Muhammad, Auwal; Aliyu Abdulkadir, Rabiu; Salim, S. N. S.; Madugu, I. S.; Tijjani, Aminu; Aminu Yusuf, Lukman; Dauda Umar, Ibrahim; Khairi, M. T. M.

2018-01-01

BrushLess Direct Current (BLDC) motor is a multivariable and highly complex nonlinear system. Variation of internal parameter values with environment or reference signal increases the difficulty in controlling the BLDC effectively. Advanced control strategies (like model predictive control) often have to be integrated to satisfy the control desires. Enhancing or proper tuning of a conventional algorithm results in achieving the desired performance. This paper presents a performance comparison of Enhanced PID and Model Predictive Control (MPC) applied to brushless direct current motor. The simulation results demonstrated that the PSO-PID is slightly better than the PID and MPC in tracking the trajectory of the reference signal. The proposed scheme could be useful algorithms for the system.

The thermodynamics of enhanced heat transfer: a model study

International Nuclear Information System (INIS)

Hovhannisyan, Karen; Allahverdyan, Armen E

2010-01-01

Situations where a spontaneous process of energy or matter transfer is enhanced by an external device are widespread in nature (the human sweating system, enzyme catalysis, facilitated diffusion across biomembranes, industrial heat-exchangers and so on). The thermodynamics of such processes remains, however, open. Here we study enhanced heat transfer by using a model junction immersed between two thermal baths at different temperatures T h and T c (T h > T c ). The transferred heat power is enhanced via controlling the junction by means of external time-dependent fields. Provided that the spontaneous heat flow process is optimized over the junction Hamiltonian, any enhancement of this spontaneous process demands consumption and subsequent dissipation of work. The efficiency of the enhancement is defined via the increment in the heat power divided by the amount of work done. We show that this efficiency is bounded from above by T c /(T h − T c ). Formally this is identical to the Carnot bound for the efficiency of ordinary refrigerators which transfer heat from cold to hot bodies. It also shares some (but not all) physical features of the Carnot bound

76 FR 4574 - Drawbridge Operation Regulations; Oakland Inner Harbor Tidal Canal, Oakland/Alameda, CA, Schedule...

Science.gov (United States)

2011-01-26

... Boating Federation, Hanson Aggregates, Power Engineering, Harbor Bay Maritime, Dutra Group, Oakland Yacht... Association, Aeolian Yacht Club, Briar Rose Yacht Charters, Baytech Marine Service, Heinold's First and Last... Outboard Motor Shop, Waterfront Hotel-Miss Pearl's Restaurant, Encinal Yacht Club, Marina Village Inn...

33 CFR 110.190 - Tortugas Harbor, in vicinity of Garden Key, Dry Tortugas, Fla.

Science.gov (United States)

2010-07-01

... Garden Key, Dry Tortugas, Fla. 110.190 Section 110.190 Navigation and Navigable Waters COAST GUARD..., in vicinity of Garden Key, Dry Tortugas, Fla. (a) The anchorage grounds. All of Bird Key Harbor, southwest of Garden Key, bounded by the surrounding reefs and shoals and, on the northeast, by a line...

Noncoding origins of anthropoid traits and a new null model of transposon functionalization.

Science.gov (United States)

del Rosario, Ricardo C H; Rayan, Nirmala Arul; Prabhakar, Shyam

2014-09-01

Little is known about novel genetic elements that drove the emergence of anthropoid primates. We exploited the sequencing of the marmoset genome to identify 23,849 anthropoid-specific constrained (ASC) regions and confirmed their robust functional signatures. Of the ASC base pairs, 99.7% were noncoding, suggesting that novel anthropoid functional elements were overwhelmingly cis-regulatory. ASCs were highly enriched in loci associated with fetal brain development, motor coordination, neurotransmission, and vision, thus providing a large set of candidate elements for exploring the molecular basis of hallmark primate traits. We validated ASC192 as a primate-specific enhancer in proliferative zones of the developing brain. Unexpectedly, transposable elements (TEs) contributed to >56% of ASCs, and almost all TE families showed functional potential similar to that of nonrepetitive DNA. Three L1PA repeat-derived ASCs displayed coherent eye-enhancer function, thus demonstrating that the "gene-battery" model of TE functionalization applies to enhancers in vivo. Our study provides fundamental insights into genome evolution and the origins of anthropoid phenotypes and supports an elegantly simple new null model of TE exaptation. © 2014 del Rosario et al.; Published by Cold Spring Harbor Laboratory Press.

Electromagnetic modelling of Raman enhancement from nanoscale substrates: a route to estimation of the magnitude of the chemical enhancement mechanism in SERS.

Science.gov (United States)

Brown, Richard J C; Wang, Jian; Tantra, Ratna; Yardley, Rachel E; Milton, Martin J T

2006-01-01

Despite widespread use for more than two decades, the SERS phenomenon has defied accurate physical and chemical explanation. The relative contributions from electronic and chemical mechanisms are difficult to quantify and are often not reproduced under nominally similar experimental conditions. This work has used electromagnetic modelling to predict the Raman enhancement expected from three configurations: metal nanoparticles, structured metal surfaces, and sharp metal tips interacting with metal surfaces. In each case, parameters such as artefact size, artefact separation and incident radiation wavelength have been varied and the resulting electromagnetic field modelled. This has yielded an electromagnetic description of these configurations with predictions of the maximum expected Raman enhancement, and hence a prediction of the optimum substrate configuration for the SERS process. When combined with experimental observations of the dependence of Raman enhancement with changing ionic strength, the modelling results have allowed a novel estimate of the size of the chemical enhancement mechanism to be produced.

A new enhanced index tracking model in portfolio optimization with sum weighted approach

Science.gov (United States)

Siew, Lam Weng; Jaaman, Saiful Hafizah; Hoe, Lam Weng

2017-04-01

Index tracking is a portfolio management which aims to construct the optimal portfolio to achieve similar return with the benchmark index return at minimum tracking error without purchasing all the stocks that make up the index. Enhanced index tracking is an improved portfolio management which aims to generate higher portfolio return than the benchmark index return besides minimizing the tracking error. The objective of this paper is to propose a new enhanced index tracking model with sum weighted approach to improve the existing index tracking model for tracking the benchmark Technology Index in Malaysia. The optimal portfolio composition and performance of both models are determined and compared in terms of portfolio mean return, tracking error and information ratio. The results of this study show that the optimal portfolio of the proposed model is able to generate higher mean return than the benchmark index at minimum tracking error. Besides that, the proposed model is able to outperform the existing model in tracking the benchmark index. The significance of this study is to propose a new enhanced index tracking model with sum weighted apporach which contributes 67% improvement on the portfolio mean return as compared to the existing model.

Clinical and CT characteristics of surgically resected lung adenocarcinomas harboring ALK rearrangements or EGFR mutations

International Nuclear Information System (INIS)

Wang, Hua; Schabath, Matthew B.; Liu, Ying; Han, Ying; Li, Qi; Gillies, Robert J.; Ye, Zhaoxiang

2016-01-01

Purpose: To determine if clinical and CT characteristics of surgically resected lung adenocarcinomas can distinguish those harboring ALK rearrangements from EGFR mutations. Materials and methods: Patients who had surgical resection and histologically confirmed lung adenocarcinoma were enrolled, including 41 patients with ALK rearrangements and 66 patients with EGFR mutations. Eighteen categorical and six quantitative CT characteristics were used to evaluate the tumors. Differences in clinical and CT characteristics between the two groups were investigated. Results: Age (P = 0.003), histological subtypes (P < 0.001), pathological stage (P = 0.007), and five CT characteristics, including size (P < 0.001), GGO (P = 0.001), bubble-like lucency (P = 0.048), lymphadenopathy (P = 0.001), and tumor shadow disappearance rate (P = 0.005) were significantly different between patients harboring ALK rearrangements compared to patients with EGFR mutations. When we compared histologic components, a solid pattern was more common (P = 0.009) in tumors with ALK rearrangements, and lepidic and acinar patterns were more common (P < 0.001 and P = 0.040, respectively) in those with EGFR mutations. Backward elimination analyses revealed that age (OR = 0.93; 95% CI 0.89–0.98), GGO (OR = 0.14; 95% CI 0.03–0.67), and lymphadenopathy (OR = 4.15; 95% CI 1.49–11.60) were significantly associated with ALK rearrangement status. Conclusion: Our analyses revealed that clinical and CT characteristics of lung adenocarcinomas harboring ALK rearrangements were significantly different, compared with those with EGFR mutations. These differences may be related to the molecular pathology of these diseases.

Movements and foraging effort of Steller's Eiders and Harlequin Ducks wintering near Dutch Harbor, Alaska

Science.gov (United States)

Reed, J.A.; Flint, Paul L.

2007-01-01

We studied the movements and foraging effort of radio-marked Steller's Eiders (Polysticta stelleri) and Harlequin Ducks (Histrionicus histrionicus) to evaluate habitat quality in an area impacted by industrial activity near Dutch Harbor, Alaska. Foraging effort was relatively low, with Steller's Eiders foraging only 2.7 ± 0.6 (SE) hours per day and Harlequin Ducks 4.1 ± 0.5 hours per day. Low-foraging effort during periods of high-energetic demand generally suggests high food availability, and high food availability frequently corresponds with reductions in home range size. However, the winter ranges of Harlequin Ducks did not appear to be smaller than usual, with the mean range size in our study (5.5 ± 1.1 km2) similar to that reported by previous investigators. The mean size of the winter ranges of Steller's Eiders was similar (5.1 ± 1.3 km2), but no comparable estimates are available. Eutrophication of the waters near Dutch Harbor caused by seafood processing and municipal sewage effluent may have increased populations of the invertebrate prey of these sea ducks and contributed to their low-foraging effort. The threat of predation by Bald Eagles (Haliaeetus leucocephalus) that winter near Dutch Harbor may cause Steller's Eiders and Harlequin Ducks to move further offshore when not foraging, contributing to an increase in range sizes. Thus, the movement patterns and foraging behavior of these ducks likely represent a balance between the cost and benefits of wintering in a human-influenced environment.

Clinical and CT characteristics of surgically resected lung adenocarcinomas harboring ALK rearrangements or EGFR mutations

Energy Technology Data Exchange (ETDEWEB)

Wang, Hua [Department of Radiology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin (China); Schabath, Matthew B. [Department of Cancer Epidemiology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL (United States); Liu, Ying [Department of Radiology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin (China); Department of Cancer Imaging and Metabolism, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL (United States); Han, Ying [Department of Biotherapy, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin (China); Li, Qi [Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin (China); Gillies, Robert J. [Department of Cancer Imaging and Metabolism, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL (United States); Department of Radiology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL (United States); Ye, Zhaoxiang, E-mail: yezhaoxiang@163.com [Department of Radiology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin (China)

2016-11-15

Purpose: To determine if clinical and CT characteristics of surgically resected lung adenocarcinomas can distinguish those harboring ALK rearrangements from EGFR mutations. Materials and methods: Patients who had surgical resection and histologically confirmed lung adenocarcinoma were enrolled, including 41 patients with ALK rearrangements and 66 patients with EGFR mutations. Eighteen categorical and six quantitative CT characteristics were used to evaluate the tumors. Differences in clinical and CT characteristics between the two groups were investigated. Results: Age (P = 0.003), histological subtypes (P < 0.001), pathological stage (P = 0.007), and five CT characteristics, including size (P < 0.001), GGO (P = 0.001), bubble-like lucency (P = 0.048), lymphadenopathy (P = 0.001), and tumor shadow disappearance rate (P = 0.005) were significantly different between patients harboring ALK rearrangements compared to patients with EGFR mutations. When we compared histologic components, a solid pattern was more common (P = 0.009) in tumors with ALK rearrangements, and lepidic and acinar patterns were more common (P < 0.001 and P = 0.040, respectively) in those with EGFR mutations. Backward elimination analyses revealed that age (OR = 0.93; 95% CI 0.89–0.98), GGO (OR = 0.14; 95% CI 0.03–0.67), and lymphadenopathy (OR = 4.15; 95% CI 1.49–11.60) were significantly associated with ALK rearrangement status. Conclusion: Our analyses revealed that clinical and CT characteristics of lung adenocarcinomas harboring ALK rearrangements were significantly different, compared with those with EGFR mutations. These differences may be related to the molecular pathology of these diseases.

Understanding science teacher enhancement programs: Essential components and a model

Science.gov (United States)

Spiegel, Samuel Albert

Researchers and practioners alike recognize that "the national goal that every child in the United States has access to high-quality school education in science and mathematics cannot be realized without the availability of effective professional development of teachers" (Hewson, 1997, p. 16). Further, there is a plethora of reports calling for the improvement of professional development efforts (Guskey & Huberman, 1995; Kyle, 1995; Loucks-Horsley, Hewson, Love, & Stiles, 1997). In this study I analyze a successful 3-year teacher enhancement program, one form of professional development, to: (1) identify essential components of an effective teacher enhancement program; and (2) create a model to identify and articulate the critical issues in designing, implementing, and evaluating teacher enhancement programs. Five primary sources of information were converted into data: (1) exit questionnaires, (2) exit surveys, (3) exit interview transcripts, (4) focus group transcripts, and (5) other artifacts. Additionally, a focus group was used to conduct member checks. Data were analyzed in an iterative process which led to the development of the list of essential components. The Components are categorized by three organizers: Structure (e.g., science research experience, a mediator throughout the program), Context (e.g., intensity, collaboration), and Participant Interpretation (e.g., perceived to be "safe" to examine personal beliefs and practices, actively engaged). The model is based on: (1) a 4-year study of a successful teacher enhancement program; (2) an analysis of professional development efforts reported in the literature; and (3) reflective discussions with implementors, evaluators, and participants of professional development programs. The model consists of three perspectives, cognitive, symbolic interaction, and organizational, representing different viewpoints from which to consider issues relevant to the success of a teacher enhancement program. These

Bioaccumulation of polychlorinated biphenyls and organochlorine pesticides in young-of-the-year bluefish (Pomatomus saltatrix) in the vicinity of a Superfund Site in New Bedford Harbor, Massachusetts, and in the adjacent waters.

Science.gov (United States)

Deshpande, Ashok D; Dockum, Bruce W; Cleary, Thomas; Farrington, Cameron; Wieczorek, Daniel

2013-07-15

Spatial gradients of polychlorinated biphenyls (PCBs) and organochlorine pesticides were examined in the young-of-the-year (YOY) bluefish (Pomatomus saltatrix) in the vicinity of a PCB Superfund Site in New Bedford Harbor, Massachusetts, and in the adjacent waters. PCB concentrations in bluefish varied between different locations, and also among fish from a given location. A generally decreasing gradient in PCB concentrations was evident as the bluefish were collected away from the Superfund Site. The average sum of PCB concentrations were highest for bluefish collected in the Upper Harbor between Interstate-195 Bridge and Coggeshall Street Bridge (Upper Harbor), followed by bluefish in Lower Harbor from north of Popes Island Bridge (Lower Harbor), and bluefish from Outer Harbor south of Hurricane Barrier (Outer Harbor). The levels of PCBs in bluefish from Clarks Cove and PCBs in bluefish from Buzzards Bay were similar and lowest among all bluefish specimens analyzed in the present study. Pesticide concentrations were about one order of magnitude or lower than the PCB concentrations, and the gradient of pesticide concentrations generally followed the gradient of PCB concentrations. Some of the commonly detected pesticides in the order of decreasing concentrations included DDTs and metabolites, heptachlor epoxide, endosulfan sulfate, and α-chlordane. Distribution of PCBs and organochlorine pesticides were examined in the tissues of YOY bluefish from Clarks Cove. PCBs and lipids in the brain samples of YOY bluefish were generally numerically greater than PCBs in the liver samples, but these differences were not statistically significant. PCBs and lipids in hypaxial muscle samples were numerically greater than PCBs in epaxial muscle samples, although these two groups of tissues were not statistically different. Despite the higher susceptibility of lighter PCB homologs to geophysical and biogeochemical weathering processes, the relative dominance of lighter homologs

76 FR 27970 - Safety Zone; Cape Charles Fireworks, Cape Charles Harbor, Cape Charles, VA.

Science.gov (United States)

2011-05-13

... Charles will sponsor a fireworks display on the shoreline of the navigable waters of Cape Charles City...[deg]01'30'' W (NAD 1983). This safety zone will be established in the vicinity of Cape Charles, VA...-AA00 Safety Zone; Cape Charles Fireworks, Cape Charles Harbor, Cape Charles, VA. AGENCY: Coast Guard...

Revisiting "Pearl Harbor": Resistance to Reel and Real Events in an English Language Classroom

Science.gov (United States)

Mackie, Ardiss; Norton, Bonny

2006-01-01

In this article, we draw on disruptive scenes in a postsecondary classroom to examine a critical incident concerning conflicting readings of the film "Pearl Harbor" (2001). We raise crucial questions for pedagogical work with popular film: Who speaks for whom about the meaning of a given film? Under what conditions do students resist…

75 FR 1738 - Drawbridge Operation Regulation; New Haven Harbor, Quinnipiac and Mill Rivers, CT

Science.gov (United States)

2010-01-13

...-AA09 Drawbridge Operation Regulation; New Haven Harbor, Quinnipiac and Mill Rivers, CT AGENCY: Coast... regulation governing the operation of three bridges across the Quinnipiac and Mill Rivers at New Haven... and 15 feet at mean low water. The Chapel Street Bridge at mile 0.4, across the Mill River has a...

77 FR 75917 - Drawbridge Operation Regulation; New Haven Harbor, Quinnipiac and Mill Rivers, CT

Science.gov (United States)

2012-12-26

...-AA09 Drawbridge Operation Regulation; New Haven Harbor, Quinnipiac and Mill Rivers, CT AGENCY: Coast..., mile 1.3, across the Quinnipiac River, and the Chapel Street Bridge, mile 0.4, across the Mill River..., across the Quinnipiac River, and the Chapel Street Bridge, mile 0.4, across the Mill River, to reduce the...

Morphodynamic changes as an impact of human intervention at the Ras El-Bar-Damietta Harbor coast, NW Damietta Promontory, Nile Delta, Egypt

Science.gov (United States)

El-Asmar, Hesham M.; Taha, Maysa M. N.; El-Sorogy, Abdelbaset S.

2016-12-01

Due to the absence of a national strategic plan for coastal management, the Nile Delta coast is no longer described as a fully dissipative, divergent, low-gradient beach face composed of fine to very fine sand. Instead, new patterns have emerged depending on rock type, geomorphology of the coast, direction of the shoreline in relation to waves and current, and the implemented defense measures. This study attempts to record the morphodynamic changes which occurred due to human intervention. Landsat satellite images acquired for the periods of time of 1973, 1984, 1989, 2003, and 2015 are used together with geomorphologic observations in order to monitor the changes along the coastal strip between Ras El-Bar and Damietta Harbor. This study reveals two beach segments; one of which lies to the east, it is protected with detached breakwater system, and shows average shoreline accretions of +4.73 myr-1, +5.0 myr-1, and +0.89myr-1 during the periods of 1984-1998, 1998-2003, and 2003-2015 respectively. This segment still has the geomorphologic imprints of the dissipative beach, wave divergence, low-gradient beach face, fine grained sand and spilling breakers. The second is to the west, between the detached breakwaters and the eastern jetty of the Damietta Harbor. It is an erosional segment with shoreline retreat of -7.43 myr-1, -10.90 myr-1, and -3.11myr-1 for the same periods. This segment shows intermediate "d" beach or intermediate-reflective, wave convergence, rip currents, with the characteristic steep sloped and cuspate beach face, cliffy, reworked sediments of coarse grained sands, mud clasts, discoidal gravels, shelly beach, and plunging breakings. The presence of convergent waves along this segment confirms the concept of an emergence of a new wave pattern of reversed eddy which enhances the steepness of the beach face, accelerates erosion, and increases the possibility of drowning of swimmers at Ras El-Bar resort. Under such circumstances the plunge step

Hydroxyurea enhances the activity of acyclovir and cidofovir against herpes simplex virus type 1 resistant strains harboring mutations in the thymidine kinase and/or the DNA polymerase genes.

Science.gov (United States)

Sergerie, Yan; Boivin, Guy

2008-01-01

Drug-resistant herpes simplex virus type 1 (HSV-1) recombinant strains harboring mutations in the thymidine kinase and/or the DNA polymerase genes were evaluated for their susceptibility to various antivirals in the presence of 25 microg/ml of hydroxyurea (HyU). The latter compound decreased the 50% inhibitory concentrations of acyclovir by 1.5-3.8-fold and that of cidofovir by 2.7-14.4-fold. However, HyU did not affect the susceptibilities of the various recombinant mutants to foscarnet. Hydroxyurea, a ribonucleotide reductase inhibitor, can increase the activity of nucleoside/nucleotide analogues against drug-resistant viruses.

Ecological evaluation of proposed discharge of dredged material from Oakland Harbor into ocean waters (Phase 3 B of -42-foot project). Volume 2, Appendixes

Energy Technology Data Exchange (ETDEWEB)

Kohn, N.P.; Ward, J.A.; Mayhew, H.L.; Word, J.Q.; Barrows, E.S.; Goodwin, S.M.; Lefkovitz, L.F. [Battelle/Marine Sciences Lab., Sequim, WA (United States)

1992-06-01

The Water Resources Development Act of 1986 (Public Law 99-662) authorized the US Army Corps of Engineers (USACE) San Francisco District, to deepen and widen the navigational channels of the Oakland Inner and Outer Harbors to accommodate deeper-draft vessels. The USACE is considering several disposal options for the dredged material removed during these channel improvements including open-water disposal. Dredged material proposed for open-water disposal must be evaluated to determine the potential impacts of the disposal activity on the water column and disposal site environments. The USACE requested that Battelle/Marine Sciences Laboratory (MSL) conduct studies to evaluate open-water disposal options for Oakland Harbor sediments. This request developed into the Oakland Harbor Phase III Program. This is Volume 2 of a two-volume report that presents information gathered to determine the suitability of ocean disposal of sediments dredged from Oakland Harbor. This volume contains the Appendixes (A through N), which provide details of the data analyses and full presentation of the data and results.

Neuro-fuzzy and neural network techniques for forecasting sea level in Darwin Harbor, Australia

Science.gov (United States)

Karimi, Sepideh; Kisi, Ozgur; Shiri, Jalal; Makarynskyy, Oleg

2013-03-01

Accurate predictions of sea level with different forecast horizons are important for coastal and ocean engineering applications, as well as in land drainage and reclamation studies. The methodology of tidal harmonic analysis, which is generally used for obtaining a mathematical description of the tides, is data demanding requiring processing of tidal observation collected over several years. In the present study, hourly sea levels for Darwin Harbor, Australia were predicted using two different, data driven techniques, adaptive neuro-fuzzy inference system (ANFIS) and artificial neural network (ANN). Multi linear regression (MLR) technique was used for selecting the optimal input combinations (lag times) of hourly sea level. The input combination comprises current sea level as well as five previous level values found to be optimal. For the ANFIS models, five different membership functions namely triangular, trapezoidal, generalized bell, Gaussian and two Gaussian membership function were tested and employed for predicting sea level for the next 1 h, 24 h, 48 h and 72 h. The used ANN models were trained using three different algorithms, namely, Levenberg-Marquardt, conjugate gradient and gradient descent. Predictions of optimal ANFIS and ANN models were compared with those of the optimal auto-regressive moving average (ARMA) models. The coefficient of determination, root mean square error and variance account statistics were used as comparison criteria. The obtained results indicated that triangular membership function was optimal for predictions with the ANFIS models while adaptive learning rate and Levenberg-Marquardt were most suitable for training the ANN models. Consequently, ANFIS and ANN models gave similar forecasts and performed better than the developed for the same purpose ARMA models for all the prediction intervals.

Enhanced Model for Fast Ignition

Energy Technology Data Exchange (ETDEWEB)

Mason, Rodney J. [Research Applications Corporation, Los Alamos, NM (United States)

2010-10-12

Laser Fusion is a prime candidate for alternate energy production, capable of serving a major portion of the nation's energy needs, once fusion fuel can be readily ignited. Fast Ignition may well speed achievement of this goal, by reducing net demands on laser pulse energy and timing precision. However, Fast Ignition has presented a major challenge to modeling. This project has enhanced the computer code ePLAS for the simulation of the many specialized phenomena, which arise with Fast Ignition. The improved code has helped researchers to understand better the consequences of laser absorption, energy transport, and laser target hydrodynamics. ePLAS uses efficient implicit methods to acquire solutions for the electromagnetic fields that govern the accelerations of electrons and ions in targets. In many cases, the code implements fluid modeling for these components. These combined features, "implicitness and fluid modeling," can greatly facilitate calculations, permitting the rapid scoping and evaluation of experiments. ePLAS can be used on PCs, Macs and Linux machines, providing researchers and students with rapid results. This project has improved the treatment of electromagnetics, hydrodynamics, and atomic physics in the code. It has simplified output graphics, and provided new input that avoids the need for source code access by users. The improved code can now aid university, business and national laboratory users in pursuit of an early path to success with Fast Ignition.

Characterization of differential gene expression in adrenocortical tumors harboring beta-catenin (CTNNB1) mutations.

Science.gov (United States)

Durand, Julien; Lampron, Antoine; Mazzuco, Tania L; Chapman, Audrey; Bourdeau, Isabelle

2011-07-01

Mutations of β-catenin gene (CTNNB1) are frequent in adrenocortical adenomas (AA) and adrenocortical carcinomas (ACC). However, the target genes of β-catenin have not yet been identified in adrenocortical tumors. Our objective was to identify genes deregulated in adrenocortical tumors harboring CTNNB1 genetic alterations and nuclear accumulation of β-catenin. Microarray analysis identified a dataset of genes that were differently expressed between AA with CTNNB1 mutations and wild-type (WT) tumors. Within this dataset, the expression profiles of five genes were validated by real time-PCR (RT-PCR) in a cohort of 34 adrenocortical tissues (six AA and one ACC with CTNNB1 mutations, 13 AA and four ACC with WT CTNNB1, and 10 normal adrenal glands) and two human ACC cell lines. We then studied the effects of suppressing β-catenin transcriptional activity with the T-cell factor/β-catenin inhibitors PKF115-584 and PNU74654 on gene expression in H295R and SW13 cells. RT-PCR analysis confirmed the overexpression of ISM1, RALBP1, and PDE2A and the down-regulation of PHYHIP in five of six AA harboring CTNNB1 mutations compared with WT AA (n = 13) and normal adrenal glands (n = 10). RALBP1 and PDE2A overexpression was also confirmed at the protein level by Western blotting analysis in mutated tumors. ENC1 was specifically overexpressed in three of three AA harboring CTNNB1 point mutations. mRNA expression and protein levels of RALBP1, PDE2A, and ENC1 were decreased in a dose-dependent manner in H295R cells after treatment with PKF115-584 or PNU74654. This study identified candidate genes deregulated in CTNNB1-mutated adrenocortical tumors that may lead to a better understanding of the role of the Wnt-β-catenin pathway in adrenocortical tumorigenesis.

Beyond Parasitism: Hepatic Lesions in Stranded Harbor Porpoises (Phocoena phocoena) Without Trematode (Campula oblonga) Infections

NARCIS (Netherlands)

S. Hiemstra; L. Harkema (Liesbeth); L.C.M. Wiersma (Lidewij); R.I. Keesler

2015-01-01

textabstractThe liver can be an indicator of the health of an individual or of a group, which can be especially important to identify agents that can cause disease in multiple species. To better characterize hepatic lesions in stranded harbor porpoises (Phocoena phocoena), we analyzed the livers

Dungeness crab survey for the Southwest Ocean Disposal Site off Grays Harbor, Washington, June 1990

Energy Technology Data Exchange (ETDEWEB)

Higgins, B.J.; Pearson, W.H. (Battelle/Marine Sciences Lab., Sequim, WA (United States))

1991-09-01

As part of the Grays Harbor Navigation Improvement Project, the Seattle District of the US Army Corps of Engineers has begun active use of the Southwest Ocean Disposal Site off Grays Harbor, Washington. This survey was to verify that the location of the area of high crab density observed during site selection surveys has not shifted into the Southeast Ocean Disposal Site. In June 1990, mean densities of juvenile Dungeness crab were 146 crab/ha within the disposal site and 609 crab/ha outside ad north of the disposal site. At nearshore locations outside the disposal site, juvenile crab density was 3275 crab/ha. Despite the low overall abundance, the spatial distribution of crab was such that the high crab densities in 1990 have remained outside the Southwest Ocean Disposal Site. The survey data have confirmed the appropriateness of the initial selection of the disposal site boundaries and indicated no need to move to the second monitoring tier. 8 refs., 9 figs., 2 tabs.

The War on Cancer: Cold Spring Harbor Laboratory Is Fighting the Good Fight.

Science.gov (United States)

Mertz, Leslie

2017-01-01

Located on the north shore of Long Island in New York, Cold Spring Harbor Laboratory (Figure 1) started out with a marine biology emphasis at the end of the 19th century, but it soon established itself as a prominent cancer research facility. That strong emphasis on cancer work continues today as this private, not-for-profit research institution enters its 127th year (Figure 2).

Cladophora (Chlorophyta) spp. Harbor Human Bacterial Pathogens in Nearshore Water of Lake Michigan†

Science.gov (United States)

Ishii, Satoshi; Yan, Tao; Shively, Dawn A.; Byappanahalli, Muruleedhara N.; Whitman, Richard L.; Sadowsky, Michael J.

2006-01-01

Cladophora glomerata, a macrophytic green alga, is commonly found in the Great Lakes, and significant accumulations occur along shorelines during the summer months. Recently, Cladophora has been shown to harbor high densities of the fecal indicator bacteria Escherichia coli and enterococci. Cladophora may also harbor human pathogens; however, until now, no studies to address this question have been performed. In the present study, we determined whether attached Cladophora, obtained from the Lake Michigan and Burns Ditch (Little Calumet River, Indiana) sides of a breakwater during the summers of 2004 and 2005, harbored the bacterial pathogens Shiga toxin-producing Escherichia coli (STEC), Salmonella, Shigella, and Campylobacter. The presence of potential pathogens and numbers of organisms were determined by using cultural methods and by using conventional PCR, most-probable-number PCR (MPN-PCR), and quantitative PCR (QPCR) performed with genus- and toxin-specific primers and probes. While Shigella and STEC were detected in 100% and 25%, respectively, of the algal samples obtained near Burns Ditch in 2004, the same pathogens were not detected in samples collected in 2005. MPN-PCR and QPCR allowed enumeration of Salmonella in 40 to 80% of the ditch- and lakeside samples, respectively, and the densities were up to 1.6 × 103 cells per g Cladophora. Similarly, these PCR methods allowed enumeration of up to 5.4 × 102 Campylobacter cells/g Cladophora in 60 to 100% of lake- and ditchside samples. The Campylobacter densities were significantly higher (P Cladophora samples than in the ditchside Cladophora samples. DNA fingerprint analyses indicated that genotypically identical Salmonella isolates were associated with geographically and temporally distinct Cladophora samples. However, Campylobacter isolates were genetically diverse. Since animal hosts are thought to be the primary habitat for Campylobacter and Salmonella species, our results suggest that Cladophora is a

Cladophora (Chlorophyta) spp. harbor human bacterial pathogens in nearshore water of Lake Michigan.

Science.gov (United States)

Ishii, Satoshi; Yan, Tao; Shively, Dawn A; Byappanahalli, Muruleedhara N; Whitman, Richard L; Sadowsky, Michael J

2006-07-01

Cladophora glomerata, a macrophytic green alga, is commonly found in the Great Lakes, and significant accumulations occur along shorelines during the summer months. Recently, Cladophora has been shown to harbor high densities of the fecal indicator bacteria Escherichia coli and enterococci. Cladophora may also harbor human pathogens; however, until now, no studies to address this question have been performed. In the present study, we determined whether attached Cladophora, obtained from the Lake Michigan and Burns Ditch (Little Calumet River, Indiana) sides of a breakwater during the summers of 2004 and 2005, harbored the bacterial pathogens Shiga toxin-producing Escherichia coli (STEC), Salmonella, Shigella, and Campylobacter. The presence of potential pathogens and numbers of organisms were determined by using cultural methods and by using conventional PCR, most-probable-number PCR (MPN-PCR), and quantitative PCR (QPCR) performed with genus- and toxin-specific primers and probes. While Shigella and STEC were detected in 100% and 25%, respectively, of the algal samples obtained near Burns Ditch in 2004, the same pathogens were not detected in samples collected in 2005. MPN-PCR and QPCR allowed enumeration of Salmonella in 40 to 80% of the ditch- and lakeside samples, respectively, and the densities were up to 1.6 x 10(3) cells per g Cladophora. Similarly, these PCR methods allowed enumeration of up to 5.4 x 10(2) Campylobacter cells/g Cladophora in 60 to 100% of lake- and ditchside samples. The Campylobacter densities were significantly higher (P Cladophora samples than in the ditchside Cladophora samples. DNA fingerprint analyses indicated that genotypically identical Salmonella isolates were associated with geographically and temporally distinct Cladophora samples. However, Campylobacter isolates were genetically diverse. Since animal hosts are thought to be the primary habitat for Campylobacter and Salmonella species, our results suggest that Cladophora is a

CC8 MRSA strains harboring SCCmec type IVc are predominant in Colombian hospitals.

Directory of Open Access Journals (Sweden)

J Natalia Jiménez

Full Text Available BACKGROUND: Recent reports highlight the incursion of community-associated MRSA within healthcare settings. However, knowledge of this phenomenon remains limited in Latin America. The aim of this study was to evaluate the molecular epidemiology of MRSA in three tertiary-care hospitals in Medellín, Colombia. METHODS: An observational cross-sectional study was conducted from 2008-2010. MRSA infections were classified as either community-associated (CA-MRSA or healthcare-associated (HA-MRSA, with HA-MRSA further classified as hospital-onset (HAHO-MRSA or community-onset (HACO-MRSA according to standard epidemiological definitions established by the U.S. Centers for Disease Control and Prevention (CDC. Genotypic analysis included SCCmec typing, spa typing, PFGE and MLST. RESULTS: Out of 538 total MRSA isolates, 68 (12.6% were defined as CA-MRSA, 243 (45.2% as HACO-MRSA and 227 (42.2% as HAHO-MRSA. The majority harbored SCCmec type IVc (306, 58.7%, followed by SCCmec type I (174, 33.4%. The prevalence of type IVc among CA-, HACO- and HAHO-MRSA isolates was 92.4%, 65.1% and 43.6%, respectively. From 2008 to 2010, the prevalence of type IVc-bearing strains increased significantly, from 50.0% to 68.2% (p = 0.004. Strains harboring SCCmec IVc were mainly associated with spa types t1610, t008 and t024 (MLST clonal complex 8, while PFGE confirmed that the t008 and t1610 strains were closely related to the USA300-0114 CA-MRSA clone. Notably, strains belonging to these three spa types exhibited high levels of tetracycline resistance (45.9%. CONCLUSION: CC8 MRSA strains harboring SCCmec type IVc are becoming predominant in Medellín hospitals, displacing previously reported CC5 HA-MRSA clones. Based on shared characteristics including SCCmec IVc, absence of the ACME element and tetracycline resistance, the USA300-related isolates in this study are most likely related to USA300-LV, the recently-described 'Latin American variant' of USA300.

Brain transcriptomes of harbor seals demonstrate gene expression patterns of animals undergoing a metabolic disease and a viral infection

Directory of Open Access Journals (Sweden)

Stephanie M. Rosales

2016-12-01

Full Text Available Diseases of marine mammals can be difficult to diagnose because of their life history and protected status. Stranded marine mammals have been a particularly useful resource to discover and comprehend the diseases that plague these top predators. Additionally, advancements in high-throughput sequencing (HTS has contributed to the discovery of novel pathogens in marine mammals. In this study, we use a combination of HTS and stranded harbor seals (Phoca vitulina to better understand a known and unknown brain disease. To do this, we used transcriptomics to evaluate brain tissues from seven neonatal harbor seals that expired from an unknown cause of death (UCD and compared them to four neonatal harbor seals that had confirmed phocine herpesvirus (PhV-1 infections in the brain. Comparing the two disease states we found that UCD animals showed a significant abundance of fatty acid metabolic transcripts in their brain tissue, thus we speculate that a fatty acid metabolic dysregulation contributed to the death of these animals. Furthermore, we were able to describe the response of four young harbor seals with PhV-1 infections in the brain. PhV-1 infected animals showed a significant ability to mount an innate and adaptive immune response, especially to combat viral infections. Our data also suggests that PhV-1 can hijack host pathways for DNA packaging and exocytosis. This is the first study to use transcriptomics in marine mammals to understand host and viral interactions and assess the death of stranded marine mammals with an unknown disease. Furthermore, we show the value of applying transcriptomics on stranded marine mammals for disease characterization.

Investigation of polycyclic aromatic hydrocarbons (PAHs) and cyclic terpenoid biomarkers in the sediments of fishing harbors in Taiwan

International Nuclear Information System (INIS)

Kao, Nien-Hsin; Su, Ming-Chien; Fan, Jheng-Rong; Yen, Chih-Chun

2015-01-01

Highlights: • Biomarkers in three fishing harbors were investigated and identified. • 17 terpanes, 10 steranes and 10 bicyclic sesquiterpanes were quantified. • Marine diesel and the three kinds of lubricants were studied. • The study can be applied to other harbors to identify oil products in sediments. - Abstract: Three fishing harbors were investigated to study the polycyclic aromatic hydrocarbons in the sediments and trace possible anthropogenic sources by identification of cyclic terpenoid biomarkers. Seventeen terpanes, 10 steranes and 10 bicyclic sesquiterpanes in the marine diesel and the three kinds of lubricants that are mainly used by fishing boats were identified and quantified. Eighteen biomarker diagnostic ratios are suggested and the correlation coefficients among the lubricants and sediment samples have the R 2 value greater than 0.73. Analyzed 16 PAHs in the sediment shows non-normal distributions and the Kruskal Wallis Test shows the significant differences (p value smaller than 0.05) with the greatest variability in benzo[g,h,i]perylene which more than 84% of the effective size (E.S.) is accounted. X-ray Photoelectron Spectroscopy (XPS) analysis was applied and the Kruskal Wallis Test shows a significant difference (p value smaller than 0.05) among certain atoms with the effective size greater than 60%

Untranslated regions of diverse plant viral RNAs vary greatly in translation enhancement efficiency

Directory of Open Access Journals (Sweden)

Fan Qiuling

2012-05-01

Full Text Available Abstract Background Whole plants or plant cell cultures can serve as low cost bioreactors to produce massive amounts of a specific protein for pharmacological or industrial use. To maximize protein expression, translation of mRNA must be optimized. Many plant viral RNAs harbor extremely efficient translation enhancers. However, few of these different translation elements have been compared side-by-side. Thus, it is unclear which are the most efficient translation enhancers. Here, we compare the effects of untranslated regions (UTRs containing translation elements from six plant viruses on translation in wheat germ extract and in monocotyledenous and dicotyledenous plant cells. Results The highest expressing uncapped mRNAs contained viral UTRs harboring Barley yellow dwarf virus (BYDV-like cap-independent translation elements (BTEs. The BYDV BTE conferred the most efficient translation of a luciferase reporter in wheat germ extract and oat protoplasts, while uncapped mRNA containing the BTE from Tobacco necrosis virus-D translated most efficiently in tobacco cells. Capped mRNA containing the Tobacco mosaic virus omega sequence was the most efficient mRNA in tobacco cells. UTRs from Satellite tobacco necrosis virus, Tomato bushy stunt virus, and Crucifer-infecting tobamovirus (crTMV did not stimulate translation efficiently. mRNA with the crTMV 5′ UTR was unstable in tobacco protoplasts. Conclusions BTEs confer the highest levels of translation of uncapped mRNAs in vitro and in vivo, while the capped omega sequence is most efficient in tobacco cells. These results provide a basis for understanding mechanisms of translation enhancement, and for maximizing protein synthesis in cell-free systems, transgenic plants, or in viral expression vectors.

A Model for Using Reflection to Enhance Interprofessional Education

OpenAIRE

Zarezadeh, Yadolah; Pearson, Pauline; Dickinson, Clair

2009-01-01

Both Reflective Practice and Interprofessional Education (IPE) have gained a considerable attention in the past three decades. Although a plethora of literature exists on either topic, few articles address the issue of using reflective techniques to enhance IPE (King &Ross, 2003; Ross et al, 2005; Goosey & Barr, 2002; Craddock, O'Halloran, Borthwick, & McPherson, 2006) and fewer provide a model to achieve this. The aim of this article is to propose a simple model for employing reflection in t...

Jam Formation of Traffic Flow in Harbor Tunnel

International Nuclear Information System (INIS)

He Hongdi; Lu Weizhen; Dong Liyun

2011-01-01

This paper reports a study concerning occurrence and growth of traffic jam in a harbor tunnel. The single-lane with three sections (downgrade, flat, and upgrade) is taken into account and they are characterized with different velocity limit. At the low density, the traffic current increases linearly with density and saturates at some values of immediately density. As the density increases, the traffic jam appears firstly before the upgrade section and then extends to the downgrade section. Additionally, the relationships of the velocity and headway against position in different densities are obtained from simulation. These results clearly clarify where and when the traffic jam appears. Finally, the critical densities are derived via the theoretical analysis before and after the discontinuous fronts and the theoretical results are consistent with the critical values of simulation results. (interdisciplinary physics and related areas of science and technology)

Reply to comment on 'Model calculation of the scanned field enhancement factor of CNTs'

International Nuclear Information System (INIS)

Ahmad, Amir; Tripathi, V K

2010-01-01

In the paper (Ahmad and Tripathi 2006 Nanotechnology 17 3798), we derived an expression to compute the field enhancement factor of CNTs under any positional distribution of CNTs by using the model of a floating sphere between parallel anode and cathode plates. Using this expression we can compute the field enhancement factor of a CNT in a cluster (non-uniformly distributed CNTs). This expression was used to compute the field enhancement factor of a CNT in an array (uniformly distributed CNTs). We used an approximation to calculate the field enhancement factor. Hence, our expressions are correct in that assumption only. Zhbanov et al (2010 Nanotechnology 21 358001) suggest a correction that can calculate the field enhancement factor without using the approximation. Hence, this correction can improve the applicability of this model. (reply)

"Tugboat Annie:" nursing's hero of Pearl Harbor--Grace Lally (1897-1983).

Science.gov (United States)

Hawkins, J W; Matthews, I

1991-01-01

In 1991 we will mark the 50th anniversary of the Japanese attack on Pearl Harbor. Few nurses know that the Chief Nurse aboard the USS Solace, the only hospital ship in port on that fateful day, played a critical role in caring for the survivors. Grace Lally's calm, professional manner, along with her sensitivity to the needs of her patients, her 12 nurses, and the crew of the Solace made her a hero. This study is an investigation of her role on December 7, 1941, and her contributions as a career military nurse.

Evaluation of Proposed Dredged Material Disposal Alternatives for New York/New Jersey Harbor. Phase 1

Science.gov (United States)

1988-08-01

been developed ( Westerdahl and Skogerboe 1981) and is being used x..-’. to predict surface runoff water quality from dredged material as part of the...Islands in New York Harbor," Miscellaneous Paper D-88-3, US Army Engineer Waterways Experiment Station, Vicksburg, MS. Westerdahl , H. E., and Skogerboe

77 FR 31067 - Cleveland Commercial Railroad Company, LLC-Continuance in Control Exemption-Cleveland Harbor Belt...

Science.gov (United States)

2012-05-24

... becoming a Class III rail carrier. CCR has established CHB as a limited liability company and has the... Commercial Railroad Company, LLC--Continuance in Control Exemption--Cleveland Harbor Belt Railroad Cleveland Commercial Railroad Company, LLC (CCR), a Class III rail carrier, has filed a verified notice of exemption...

Contribution of Interstitial Deletion of 21q22.2-3 per se to Prostate Cancer Progression in Tumors Harboring TMPRSS2-ERG Translocations

Science.gov (United States)

2015-12-01

harboring TMPRSS2- ERG translocations PRINCIPAL INVESTIGATOR: Yan Dong CONTRACTING ORGANIZATION: Tulane University New Orleans, LA 70112...0485 to prostate cancer progression in tumors harboring TMPRSS2- ERG translocations 5b. GRANT NUMBER W81XWH-14-1-0485 5c. PROGRAM ELEMENT NUMBER...Unlimited 13. SUPPLEMENTARY NOTES 14. ABSTRACT TMPRSS2- ERG gene fusions are present in close to 50% of human prostate cancers. Approximately half of the

Strategies to Enhance Online Learning Teams. Team Assessment and Diagnostics Instrument and Agent-based Modeling

Science.gov (United States)

2010-08-12

Strategies to Enhance Online Learning Teams Team Assessment and Diagnostics Instrument and Agent-based Modeling Tristan E. Johnson, Ph.D. Learning ...REPORT DATE AUG 2010 2. REPORT TYPE 3. DATES COVERED 00-00-2010 to 00-00-2010 4. TITLE AND SUBTITLE Strategies to Enhance Online Learning ...TeamsTeam Strategies to Enhance Online Learning Teams: Team Assessment and Diagnostics Instrument and Agent-based Modeling 5a. CONTRACT NUMBER 5b. GRANT

Foam for Enhanced Oil Recovery : Modeling and Analytical Solutions

NARCIS (Netherlands)

Ashoori, E.

2012-01-01

Foam increases sweep in miscible- and immiscible-gas enhanced oil recovery by decreasing the mobility of gas enormously. This thesis is concerned with the simulations and analytical solutions for foam flow for the purpose of modeling foam EOR in a reservoir. For the ultimate goal of upscaling our

LEARNING SEMANTICS-ENHANCED LANGUAGE MODELS APPLIED TO UNSUEPRVISED WSD

Energy Technology Data Exchange (ETDEWEB)

VERSPOOR, KARIN [Los Alamos National Laboratory; LIN, SHOU-DE [Los Alamos National Laboratory

2007-01-29

An N-gram language model aims at capturing statistical syntactic word order information from corpora. Although the concept of language models has been applied extensively to handle a variety of NLP problems with reasonable success, the standard model does not incorporate semantic information, and consequently limits its applicability to semantic problems such as word sense disambiguation. We propose a framework that integrates semantic information into the language model schema, allowing a system to exploit both syntactic and semantic information to address NLP problems. Furthermore, acknowledging the limited availability of semantically annotated data, we discuss how the proposed model can be learned without annotated training examples. Finally, we report on a case study showing how the semantics-enhanced language model can be applied to unsupervised word sense disambiguation with promising results.

Heat from harbor to city; Warmte van haven naar stad

Energy Technology Data Exchange (ETDEWEB)

Roggen, M. (ed.)

2004-09-01

The Rotterdam harbor area is perfect for an industrial heat distribution network. Numerous major heat producers and many potential consumers of all sizes group together in this industrial area. The new Heat Company ('Warmtebedrijf') is making a detailed business plan to build a heat distribution system. [Dutch] Als er een regio is waar een industrieel warmtenet voor de hand ligt, is het het Rotterdamse havengebied wel. Tal van grote warmteproducenten en een woud aan kleinere en grotere afnemers zijn praktisch buren. Om handen en voeten te geven aan dit op papier mooie idee, stelt het kersverse Warmtebedrijf io een gedetailleerd businessplan op.

The combination of colesevelam with sitagliptin enhances glycemic control in diabetic ZDF rat model

DEFF Research Database (Denmark)

Shang, Quan; Liu, Matthew K; Saumoy, Monica

2012-01-01

. In the present study, we tested whether adding sitagliptin (Januvia) (SIT), which prolongs bioactive GLP-1 half life, to COL would further enhance glycemic control. Male Zucker diabetic fatty (ZDF) rats were assigned to four groups: diabetic model without treatment (the model), the model treated with 2% COL or 0...... to levels similar to the model. Histological examination of the pancreatic ß-cell islets showed that islet sizes were larger, proliferation enhanced, and cell apoptosis reduced in the COL+SIT but not the SIT alone group compared with the model. We hypothesize that the combination of COL with SIT extends...

Novel B19-like parvovirus in the brain of a harbor seal.

Directory of Open Access Journals (Sweden)

Rogier Bodewes

Full Text Available Using random PCR in combination with next-generation sequencing, a novel parvovirus was detected in the brain of a young harbor seal (Phoca vitulina with chronic non-suppurative meningo-encephalitis that was rehabilitated at the Seal Rehabilitation and Research Centre (SRRC in the Netherlands. In addition, two novel viruses belonging to the family Anelloviridae were detected in the lungs of this animal. Phylogenetic analysis of the coding sequence of the novel parvovirus, tentatively called Seal parvovirus, indicated that this virus belonged to the genus Erythrovirus, to which human parvovirus B19 also belongs. Although no other seals with similar signs were rehabilitated in SRRC in recent years, a prevalence study of tissues of seals from the same area collected in the period 2008-2012 indicated that the Seal parvovirus has circulated in the harbor seal population at least since 2008. The presence of the Seal parvovirus in the brain was confirmed by real-time PCR and in vitro replication. Using in situ hybridization, we showed for the first time that a parvovirus of the genus Erythrovirus was present in the Virchow-Robin space and in cerebral parenchyma adjacent to the meninges. These findings showed that a parvovirus of the genus Erythrovirus can be involved in central nervous system infection and inflammation, as has also been suspected but not proven for human parvovirus B19 infection.

Novel B19-like parvovirus in the brain of a harbor seal.

Science.gov (United States)

Bodewes, Rogier; Rubio García, Ana; Wiersma, Lidewij C M; Getu, Sarah; Beukers, Martijn; Schapendonk, Claudia M E; van Run, Peter R W A; van de Bildt, Marco W G; Poen, Marjolein J; Osinga, Nynke; Sánchez Contreras, Guillermo J; Kuiken, Thijs; Smits, Saskia L; Osterhaus, Albert D M E

2013-01-01

Using random PCR in combination with next-generation sequencing, a novel parvovirus was detected in the brain of a young harbor seal (Phoca vitulina) with chronic non-suppurative meningo-encephalitis that was rehabilitated at the Seal Rehabilitation and Research Centre (SRRC) in the Netherlands. In addition, two novel viruses belonging to the family Anelloviridae were detected in the lungs of this animal. Phylogenetic analysis of the coding sequence of the novel parvovirus, tentatively called Seal parvovirus, indicated that this virus belonged to the genus Erythrovirus, to which human parvovirus B19 also belongs. Although no other seals with similar signs were rehabilitated in SRRC in recent years, a prevalence study of tissues of seals from the same area collected in the period 2008-2012 indicated that the Seal parvovirus has circulated in the harbor seal population at least since 2008. The presence of the Seal parvovirus in the brain was confirmed by real-time PCR and in vitro replication. Using in situ hybridization, we showed for the first time that a parvovirus of the genus Erythrovirus was present in the Virchow-Robin space and in cerebral parenchyma adjacent to the meninges. These findings showed that a parvovirus of the genus Erythrovirus can be involved in central nervous system infection and inflammation, as has also been suspected but not proven for human parvovirus B19 infection.

33 CFR 165.906 - Lakeside Yacht Club in Cleveland Harbor, Cleveland, OH-regulated navigation areas.

Science.gov (United States)

2010-07-01

... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Lakeside Yacht Club in Cleveland... Guard District § 165.906 Lakeside Yacht Club in Cleveland Harbor, Cleveland, OH—regulated navigation... portion of the vessel extending above the waterline. All of these areas are inside the “Lakeside Yacht...

75 FR 56130 - Pearl Harbor National Wildlife Refuge, Honolulu County, HI; Comprehensive Conservation Plan and...

Science.gov (United States)

2010-09-15

... DEPARTMENT OF THE INTERIOR Fish and Wildlife Service [FWS-R1-R-2010-N160; 1265-0000-10137-S3] Pearl Harbor National Wildlife Refuge, Honolulu County, HI; Comprehensive Conservation Plan and... Kamehameha Highway, Room 2C, Hale`iwa, HI 96712. Alternatively, you may fax comments to the refuge at (808...

78 FR 52753 - Taking of Marine Mammals Incidental to Commercial Fishing Operations; Harbor Porpoise Take...

Science.gov (United States)

2013-08-26

... strategy enacted in 2010 based on deliberations by the Harbor Porpoise Take Reduction Team. This action is... information (e.g., name, address, etc.), confidential business information, or otherwise sensitive information... Protection Act (MMPA) to reduce the level of serious injury and mortality of the Gulf of Maine/Bay of Fundy...

Enhanced air dispersion modelling at a typical Chinese nuclear power plant site: Coupling RIMPUFF with two advanced diagnostic wind models.

Science.gov (United States)

Liu, Yun; Li, Hong; Sun, Sida; Fang, Sheng

2017-09-01

An enhanced air dispersion modelling scheme is proposed to cope with the building layout and complex terrain of a typical Chinese nuclear power plant (NPP) site. In this modelling, the California Meteorological Model (CALMET) and the Stationary Wind Fit and Turbulence (SWIFT) are coupled with the Risø Mesoscale PUFF model (RIMPUFF) for refined wind field calculation. The near-field diffusion coefficient correction scheme of the Atmospheric Relative Concentrations in the Building Wakes Computer Code (ARCON96) is adopted to characterize dispersion in building arrays. The proposed method is evaluated by a wind tunnel experiment that replicates the typical Chinese NPP site. For both wind speed/direction and air concentration, the enhanced modelling predictions agree well with the observations. The fraction of the predictions within a factor of 2 and 5 of observations exceeds 55% and 82% respectively in the building area and the complex terrain area. This demonstrates the feasibility of the new enhanced modelling for typical Chinese NPP sites. Copyright © 2017 Elsevier Ltd. All rights reserved.

An entrepreneurial training model to enhance undergraduate training in biomedical research.

Science.gov (United States)

Kamangar, Farin; Silver, Gillian; Hohmann, Christine; Hughes-Darden, Cleo; Turner-Musa, Jocelyn; Haines, Robert Trent; Jackson, Avis; Aguila, Nelson; Sheikhattari, Payam

2017-01-01

Undergraduate students who are interested in biomedical research typically work on a faculty member's research project, conduct one distinct task (e.g., running gels), and, step by step, enhance their skills. This "apprenticeship" model has been helpful in training many distinguished scientists over the years, but it has several potential drawbacks. For example, the students have limited autonomy, and may not understand the big picture, which may result in students giving up on their goals for a research career. Also, the model is costly and may greatly depend on a single mentor. The NIH Building Infrastructure Leading to Diversity (BUILD) Initiative has been established to fund innovative undergraduate research training programs and support institutional and faculty development of the recipient university. The training model at Morgan State University (MSU), namely " A S tudent- C entered En trepreneurship D evelopment training model" (ASCEND), is one of the 10 NIH BUILD-funded programs, and offers a novel, experimental "entrepreneurial" training approach. In the ASCEND training model, the students take the lead. They own the research, understand the big picture, and experience the entire scope of the research process, which we hypothesize will lead to a greater sense of self-efficacy and research competency, as well as an enhanced sense of science identity. They are also immersed in environments with substantial peer support, where they can exchange research ideas and share experiences. This is important for underrepresented minority students who might have fewer role models and less peer support in conducting research. In this article, we describe the MSU ASCEND entrepreneurial training model's components, rationale, and history, and how it may enhance undergraduate training in biomedical research that may be of benefit to other institutions. We also discuss evaluation methods, possible sustainability solutions, and programmatic challenges that can affect all

A Rotational Blended Learning Model: Enhancement and Quality Assurance

Science.gov (United States)

Ghoul, Said

2013-01-01

Research on blended learning theory and practice is growing nowadays with a focus on the development, evaluation, and quality assurance of case studies. However, the enhancement of blended learning existing models, the specification of their online parts, and the quality assurance related specifically to them have not received enough attention.…

The Development of KHI'S Business Model to Enhance Sustainable Competitive Advantage

OpenAIRE

Firdaus, Anton; Hamsal, Mohammad

2013-01-01

This research about how PT KHI make an improvement in Business model by offering EPC (Engineering, Procurement & Construction) services to costumer to gain a better profit margin. Research result suggests PT KHI to improve its Business Model in order to enhance sustainable competitive advantage. Many competitors have the same business model in industry. PT KHI should offer different business model to stay ahead and winning the competition. The research result indicate based on current con...

CO2 Mass transfer model for carbonic anhydrase-enhanced aqueous MDEA solutions

DEFF Research Database (Denmark)

Gladis, Arne Berthold; Deslauriers, Maria Gundersen; Neerup, Randi

2018-01-01

In this study a CO2 mass transfer model was developed for carbonic anhydrase-enhanced MDEA solutions based on a mechanistic kinetic enzyme model. Four different enzyme models were compared in their ability to predict the liquid side mass transfer coefficient at temperatures in the range of 298...

An integrated numerical and physical modeling system for an enhanced in situ bioremediation process

International Nuclear Information System (INIS)

Huang, Y.F.; Huang, G.H.; Wang, G.Q.; Lin, Q.G.; Chakma, A.

2006-01-01

Groundwater contamination due to releases of petroleum products is a major environmental concern in many urban districts and industrial zones. Over the past years, a few studies were undertaken to address in situ bioremediation processes coupled with contaminant transport in two- or three-dimensional domains. However, they were concentrated on natural attenuation processes for petroleum contaminants or enhanced in situ bioremediation processes in laboratory columns. In this study, an integrated numerical and physical modeling system is developed for simulating an enhanced in situ biodegradation (EISB) process coupled with three-dimensional multiphase multicomponent flow and transport simulation in a multi-dimensional pilot-scale physical model. The designed pilot-scale physical model is effective in tackling natural attenuation and EISB processes for site remediation. The simulation results demonstrate that the developed system is effective in modeling the EISB process, and can thus be used for investigating the effects of various uncertainties. - An integrated modeling system was developed to enhance in situ bioremediation processes

76 FR 55413 - Proposed Safe Harbor Agreement for California Red-legged Frog, California Tiger Salamander, Smith...

Science.gov (United States)

2011-09-07

...] Proposed Safe Harbor Agreement for California Red-legged Frog, California Tiger Salamander, Smith's Blue... endangered Smith's blue butterfly (Euphilotes enoptes smithi) under the Endangered Species Act of 1973, as..., California tiger salamander, Smith's blue butterfly, and Yadon's piperia on the property subject to the...

76 FR 12 - Drawbridge Operation Regulations; New Haven Harbor, Quinnipiac and Mill Rivers, New Haven, CT

Science.gov (United States)

2011-01-03

... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 117 [Docket No. USCG-2010-1096] Drawbridge Operation Regulations; New Haven Harbor, Quinnipiac and Mill Rivers, New Haven, CT AGENCY: Coast Guard, DHS. ACTION: Notice of temporary deviation from regulations. SUMMARY: The Commander, First Coast Guard...

Host characteristics and infection level of an intestinal parasite Corynosoma strumosum (Acanthocephala) in the Kuril harbor seal of Erimo Cape, Hokkaido, Japan.

Science.gov (United States)

Kaimoto, Tadashi; Hirazawa, Takuya; Masubuchi, Takahito; Morohoshi, Aya; Katahira, Hirotaka; Kobayashi, Mari

2018-04-01

The Kuril harbor seal around Hokkaido is presently recovering from a resource crisis while conflicts with local fisheries have become a concern. However, its feeding habits, which are fundamental information for taking proper preventive measures, are still poorly understood. We thus examined the infection status of a trophically-transmitted parasite, Corynosoma strumosum in the seals of Erimo Cape, to assess the host's feeding habits with a practical view of the parasite as a biological indicator. A total of 2802 worms were found from 20 male and 20 female by-caught animals in salmon set nets within local fisheries during August to November 2014. The parasite abundance was explained mainly by the host's developmental stage and intestinal length while weakly affected by gender and body size, through an estimation of generalized linear models combined with hierarchical partitioning. Considering the past records that demersal fishes are the probable main sources of infection, the infection level may owe to individual host differences regarding these sources and/or feeding grounds with relating the host characteristics. This supports that the resource management of Kuril harbor seals requires careful consideration of the individual differences in feeding behavior. Copyright © 2017 Elsevier B.V. All rights reserved.

3 CFR 8463 - Proclamation 8463 of December 4, 2009. National Pearl Harbor Remembrance Day, 2009

Science.gov (United States)

2010-01-01

... who selflessly served our Nation at home and abroad during World War II. On a tranquil Sunday morning, as war raged around the globe, the attack on Pearl Harbor effectively ended American isolation... have hereunto set my hand this fourth day of December, in the year of our Lord two thousand nine, and...

Mitigation of Shore Damage Attributed to the Federal Navigation Structures at Port Sanilac Harbor, Michigan.

Science.gov (United States)

1975-09-01

produces waves with nealioible wace heights (i.e., 0.5 feet); (b) all waves were deep water waves with no correction for shallow water; and, (c) the...at that harbor. This would re- sult in a loss to the local economy . Ini addition, littoral drift would resume southward thus affecting the littoral

Cold Spring Harbor symposia on quantitative biology: Volume 51, Molecular biology of /ital Homo sapiens/

International Nuclear Information System (INIS)

1986-01-01

This volume is the second part of a collection of papers submitted by the participants to the 1986 Cold Spring Harbor Symposium on Quantitative Biology entitled Molecular Biology of /ital Homo sapiens/. The 49 papers included in this volume are grouped by subject into receptors, human cancer genes, and gene therapy. (DT)

Tracer kinetic model-driven registration for dynamic contrast-enhanced MRI time-series data.

Science.gov (United States)

Buonaccorsi, Giovanni A; O'Connor, James P B; Caunce, Angela; Roberts, Caleb; Cheung, Sue; Watson, Yvonne; Davies, Karen; Hope, Lynn; Jackson, Alan; Jayson, Gordon C; Parker, Geoffrey J M

2007-11-01

Dynamic contrast-enhanced MRI (DCE-MRI) time series data are subject to unavoidable physiological motion during acquisition (e.g., due to breathing) and this motion causes significant errors when fitting tracer kinetic models to the data, particularly with voxel-by-voxel fitting approaches. Motion correction is problematic, as contrast enhancement introduces new features into postcontrast images and conventional registration similarity measures cannot fully account for the increased image information content. A methodology is presented for tracer kinetic model-driven registration that addresses these problems by explicitly including a model of contrast enhancement in the registration process. The iterative registration procedure is focused on a tumor volume of interest (VOI), employing a three-dimensional (3D) translational transformation that follows only tumor motion. The implementation accurately removes motion corruption in a DCE-MRI software phantom and it is able to reduce model fitting errors and improve localization in 3D parameter maps in patient data sets that were selected for significant motion problems. Sufficient improvement was observed in the modeling results to salvage clinical trial DCE-MRI data sets that would otherwise have to be rejected due to motion corruption. Copyright 2007 Wiley-Liss, Inc.

Incidence of plasmid-linked antibiotic-heavy metal resistant enterics in water-sediment from agricultural and harbor sites

Energy Technology Data Exchange (ETDEWEB)

Mietz, J.A.; Sjorgren, R.E.

1983-01-01

This study assesses the impact of antibiotics used in clinical and veterinary practices on the incidence of antibiotic-heavy metal resistant enterics in fresh water and sediment from agricultural and harbor sample sites. A total of 848 bacterial strains of the family Enterobacteriaceae was isolated from agricultural and lake harbor samples. These were examined for anitbiotic-heavy metal resistance. A select smaller number of these isolates were also examined for the presence of plasmids and ability to transfer antibiotic resistance via conjugation or transformation. More than 85% of the 848 isolates from all four sites were resistant to Pb, Zn, and Co while 5.6% to 16% were resistant to Te and 2.4% to 5.7% to Hg. Of the total isolates tested, 87% were resistant to six or more antibiotics and 74% were also simultaneously resistant to Co, Zn, and Pb. Testing the resistance of the water isolates to antibiotics used solely in animal husbandry-veterinary medicine indicated that 55.6% of the agricultural isolates possessed resistance to these antibiotics while only 31.9% of the isolates from harbor water showed resistance to the same antibiotics. Of 41 ampicillin resistant isolates examined, 16 (39%) were capable of transferring antibiotic-heavy resistance markers via conjugation. From this same group, plasmid DNA preparations were made. Of these latter preparations, 67% transformed recipient E. coli cells while 58% possessed discernible, often multiple plasmids when examined by gel electrophoresis.

Diffraction enhanced imaging of a rat model of gastric acid aspiration pneumonitis.

Science.gov (United States)

Connor, Dean M; Zhong, Zhong; Foda, Hussein D; Wiebe, Sheldon; Parham, Christopher A; Dilmanian, F Avraham; Cole, Elodia B; Pisano, Etta D

2011-12-01

Diffraction-enhanced imaging (DEI) is a type of phase contrast x-ray imaging that has improved image contrast at a lower dose than conventional radiography for many imaging applications, but no studies have been done to determine if DEI might be useful for diagnosing lung injury. The goals of this study were to determine if DEI could differentiate between healthy and injured lungs for a rat model of gastric aspiration and to compare diffraction-enhanced images with chest radiographs. Radiographs and diffraction-enhanced chest images of adult Sprague Dawley rats were obtained before and 4 hours after the aspiration of 0.4 mL/kg of 0.1 mol/L hydrochloric acid. Lung damage was confirmed with histopathology. The radiographs and diffraction-enhanced peak images revealed regions of atelectasis in the injured rat lung. The diffraction-enhanced peak images revealed the full extent of the lung with improved clarity relative to the chest radiographs, especially in the portion of the lower lobe that extended behind the diaphragm on the anteroposterior projection. For a rat model of gastric acid aspiration, DEI is capable of distinguishing between a healthy and an injured lung and more clearly than radiography reveals the full extent of the lung and the lung damage. Copyright © 2011 AUR. All rights reserved.

Directional wave and temperature data from nine buoys in Gray's Harbor, Washington, 1994-2002 (NODC Accession 0000756)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — Wave data were collected from 9 buoys in Grays Harbor, Washington, from 01 January 1994 to 24 July 2002. Data were collected as part of the Coastal Data Information...

Central nervous system disease and genital disease in harbor porpoises (Phocoena phocoena) are associated with different herpesviruses

NARCIS (Netherlands)

C.E. van Elk; M.W.G. van de Bildt (Marco); P.R.W.A. van Run (Peter); De Jong, A. (Anton); S. Getu (Sarah); G.M.G.M. Verjans (George); A.D.M.E. Osterhaus (Albert); T. Kuiken (Thijs)

2016-01-01

textabstractHerpesvirus infection causes disease of variable severity in many species, including cetaceans. However, little is known about herpesvirus infection in harbor porpoises (Phocoena phocoena), despite being widespread in temperate coastal waters of the Northern Hemisphere. Therefore, we

Enhancing uncertainty tolerance in the modelling creep of ligaments

International Nuclear Information System (INIS)

Taha, M M Reda; Lucero, J

2006-01-01

The difficulty in performing biomechanical tests and the scarcity of biomechanical experimental databases necessitate extending the current knowledge base to allow efficient modelling using limited data sets. This study suggests a framework to reduce uncertainties in biomechanical systems using limited data sets. The study also shows how sparse data and epistemic input can be exploited using fuzzy logic to represent biomechanical relations. An example application to model collagen fibre recruitment in the medial collateral ligaments during time-dependent deformation under cyclic loading (creep) is presented. The study suggests a quality metric that can be employed to observe and enhance uncertainty tolerance in the modelling process

The virtual enhancements - solar proton event radiation (VESPER) model

Science.gov (United States)

Aminalragia-Giamini, Sigiava; Sandberg, Ingmar; Papadimitriou, Constantinos; Daglis, Ioannis A.; Jiggens, Piers

2018-02-01

A new probabilistic model introducing a novel paradigm for the modelling of the solar proton environment at 1 AU is presented. The virtual enhancements - solar proton event radiation model (VESPER) uses the European space agency's solar energetic particle environment modelling (SEPEM) Reference Dataset and produces virtual time-series of proton differential fluxes. In this regard it fundamentally diverges from the approach of existing SPE models that are based on probabilistic descriptions of SPE macroscopic characteristics such as peak flux and cumulative fluence. It is shown that VESPER reproduces well the dataset characteristics it uses, and further comparisons with existing models are made with respect to their results. The production of time-series as the main output of the model opens a straightforward way for the calculation of solar proton radiation effects in terms of time-series and the pairing with effects caused by trapped radiation and galactic cosmic rays.

Mechanical characteristics of harbor seal (Phoca vitulina) vibrissae under different circumstances and their implications on its sensing methodology

International Nuclear Information System (INIS)

Hans, H; Miao, J M; Triantafyllou, M S

2014-01-01

In this paper, the mechanical properties of harbor seal vibrissae immersed in various solutions are investigated. As there are no nerves along the length of the vibrissae, all the perturbations have to be transmitted to their bases for sensing. Hence, quantification and understanding of the mechanical properties of the vibrissae are essential in determining the perturbations transmitted to the base of the vibrissae. Two experimental setups are devised for measurements of the different properties of the vibrissae. The first experimental setup is performed with a dynamic mechanical analysis machine. The measured properties in these experiments are the modulus of elasticity and the damping of the vibrissae. Dry, saline water-immersed, water-immersed and Hanks' balanced salt solution (HBSS)-immersed vibrissae are tested to determine the effects of these solutions on the properties of the vibrissae. Tests on the duration of immersion are also performed with saline water-immersed vibrissae. The second experimental setup is performed with a mini-shaker connected to a clamp, which rigidly holds the vibrissae at their bases. The measured properties in these experiments are the natural frequencies of the vibrissae. The results indicate that the moduli of elasticity of the vibrissae are found to decrease along their lengths. However, their damping does not vary along the lengths. HBSS-immersed and saline water-immersed vibrissae show similar characteristics on their properties. An analytical model for predicting the natural frequencies of the vibrissae is also derived. Strong agreement with previous studies on the underwater sensing principle of the harbor seal is also established. (paper)

Effects of deposition of heavy-metal-polluted harbor mud on microbial diversity and metal resistance in sandy marine sediments

DEFF Research Database (Denmark)

Toes, Ann-Charlotte M; Finke, Niko; Kuenen, J Gijs

2008-01-01

Deposition of dredged harbor sediments in relatively undisturbed ecosystems is often considered a viable option for confinement of pollutants and possible natural attenuation. This study investigated the effects of deposition of heavy-metal-polluted sludge on the microbial diversity of sandy...... the finding that some groups of clones were shared between the metal-impacted sandy sediment and the harbor control, comparative analyses showed that the two sediments were significantly different in community composition. Consequences of redeposition of metal-polluted sediment were primarily underlined...... with cultivation-dependent techniques. Toxicity tests showed that the percentage of Cd- and Cu-tolerant aerobic heterotrophs was highest among isolates from the sandy sediment with metal-polluted mud on top....

Towards a moderated mediation model of innovative work behaviour enhancement

NARCIS (Netherlands)

Stoffers, Jol M.; van der Heijden, Beatrice; Notelaers, Guy L.A.

2014-01-01

Purpose – The purpose of this paper is to investigate a moderated mediation model of innovative work behaviour enhancement. Perceived firm (organizational and market) performance was assumed to moderate the relationships between leader-member exchange (LMX) and organizational citizenship behaviour

An enhanced Brinson model with modified kinetics for martensite transformation

Energy Technology Data Exchange (ETDEWEB)

Kim, Young-Jin; Lee, Jung Ju [Korea Advanced Institute of Science and Technology, Daejeon (Korea, Republic of); Jeong, Ju-Won [Korea Aerospace Research Institute, Daejeon (Korea, Republic of); Lim, Jae Hyuk [Chonbuk National University, Jeonju (Korea, Republic of)

2017-03-15

We propose an enhanced Brinson model with modified kinetics for martensite transformation. Two additional material constants are considered to follow the stress-temperature diagram above austenite start temperature (As) along with treatment to keep the continuity of the martensite volume fraction and the path dependency of the phase transformation. To demonstrate the performance of the proposed model, we implement this algorithm into ABAQUS user subroutine, then conduct several numerical simulations and compare their results with SMA wire experiments as well as those of three-dimensional SMA constitutive models. From the results, it turns out that the proposed model is as accurate as the three-dimensional models and shows better accuracy over original Brinson model in terms of recovery stress.

Comparative account of macrofouling ecology at Vijaydurg Harbor and Musakazi-Jaitapur Bay, central western coast of India

Digital Repository Service at National Institute of Oceanography (India)

Sawant, S.S.; Wagh, A.B.

The extent and the nature of fouling were studied on mild steel, aluminum, and stainless steel panels at the Vijaydurg Harbor and Musakazi-Jaitapur Bay on the central western coast of India. In addition, cathodically protected and unprotected panels...

78 FR 24069 - Safety Zone; 12th Annual Saltwater Classic; Port Canaveral Harbor; Port Canaveral, FL

Science.gov (United States)

2013-04-24

... under Executive Order 13045, Protection of Children from Environmental Health Risks and Safety Risks. This rule is not an economically significant rule and does not create an environmental risk to health...-AA00 Safety Zone; 12th Annual Saltwater Classic; Port Canaveral Harbor; Port Canaveral, FL AGENCY...

76 FR 33721 - Takes of Marine Mammals Incidental to Specified Activities; Harbor Activities Related to the...

Science.gov (United States)

2011-06-09

..., incidental to conducting Delta Mariner harbor operations for one year. NMFS reviewed the ULA application and... vitulina), and Northern elephant seals (Mirounga angustirostris) hauled out on Small Haul-out Site 1 to... lions; and 43 Northern elephant seals by Level B harassment only. Description of the Specified...

Parallel imaging enhanced MR colonography using a phantom model.

LENUS (Irish Health Repository)

Morrin, Martina M

2008-09-01

To compare various Array Spatial and Sensitivity Encoding Technique (ASSET)-enhanced T2W SSFSE (single shot fast spin echo) and T1-weighted (T1W) 3D SPGR (spoiled gradient recalled echo) sequences for polyp detection and image quality at MR colonography (MRC) in a phantom model. Limitations of MRC using standard 3D SPGR T1W imaging include the long breath-hold required to cover the entire colon within one acquisition and the relatively low spatial resolution due to the long acquisition time. Parallel imaging using ASSET-enhanced T2W SSFSE and 3D T1W SPGR imaging results in much shorter imaging times, which allows for increased spatial resolution.

A case of lung adenocarcinoma harboring EGFR mutation and EML4-ALK fusion gene

International Nuclear Information System (INIS)

Tanaka, Hisashi; Hayashi, Akihito; Morimoto, Takeshi; Taima, Kageaki; Tanaka, Yoshihito; Shimada, Michiko; Kurose, Akira; Takanashi, Shingo; Okumura, Ken

2012-01-01

Lung cancer is the leading cause of cancer-related death worldwide. Epidermal growth factor receptor (EGFR) - tyrosine kinase inhibitor (TKI) is used for the patients with EGFR-mutant lung cancer. Recently, phase III studies in the patients with EGFR-mutant demonstrated that EGFR-TKI monotherapy improved progression-free survival compared with platinum-doublet chemotherapy. The echinoderm microtubule-associated protein-like 4 (EML4) - anaplastic lymphoma kinase (ALK) fusion oncogene represents one of the newest molecular targets in non-small cell lung cancer (NSCLC). Patients who harbor EML4-ALK fusions have been associated with a lack of EGFR or KRAS mutations. We report a 39-year-old patient diagnosed as adenocarcinoma harboring EGFR mutation and EML4-ALK fusion gene. We treated this patient with erlotinib as the third line therapy, but no clinical benefit was obtained. We experienced a rare case with EGFR mutation and EML4-ALK. Any clinical benefit using EGFR-TKI was not obtained in our case. The therapeutic choice for the patients with more than one driver mutations is unclear. We needs further understanding of the lung cancer molecular biology and the biomarker infomation

78 FR 46855 - Safety Zone; East End Maritime Foundation Fireworks Display, Greenport Harbor, Greenport, NY

Science.gov (United States)

2013-08-02

... 1625-AA00 Safety Zone; East End Maritime Foundation Fireworks Display, Greenport Harbor, Greenport, NY... temporary rule proposes to establish a safety zone for the East End Maritime Foundation fireworks display....1. 0 2. Add Sec. 165.T01-0600 to read as follows: Sec. 165.T01-0600 Safety Zone; East End Maritime...

Preliminary assessment of Miramar Petrochemical Harbor as PAH source to Guajará bay (Belém-PA-Brazil surface sediments

Directory of Open Access Journals (Sweden)

Silvana do Socorro Veloso Sodré

Full Text Available Abstract A preliminary study on a petrochemical harbor as a potential source of polycyclic aromatic hydrocarbons (PAH to surface sediments of Guajará bay, located in Belém City, Pará State, Brazil, over the last 10 years is presented here. The 16 priority USEPA PAH were identified and quantified. Surface sediment samples, collected at 5 sites in the bay, near Miramar Petrochemical Harbor (TEMIR, were extracted and analyzed by high performance liquid chromatography with a diode array detector. Total PAH concentration ranged from 533.0 to 3123.3 ng g-1 dry weight with a mean concentration of 1091.9 ng g-1. The most contaminated places were those where muddy sediments were found with highest concentrations of organic matter. The priority PAH with low molecular weight represented 54.4% of the total abundance and indicate that the main source of contamination of the sediments was petrogenic, indicative of the relevant contribution of the petrochemical harbor activity to the input of PAH to Guajará bay. However, pyrolytic PAH coming from fuel combustion, household waste combustion and untreated sewage discharge are also potential contamination sources to this environment.

Anthropogenic inputs of dissolved organic matter in New York Harbor

Science.gov (United States)

Gardner, G. B.; Chen, R. F.; Olavasen, J.; Peri, F.

2016-02-01

The Hudson River flows into the Atlantic Ocean through a highly urbanized region which includes New York City to the east and Newark, New Jersey to the west. As a result, the export of Dissolved Organic Carbon (DOC) from the Hudson to the Atlantic Ocean includes a significant anthropogenic component. A series of high resolution studies of the DOC dynamics of this system were conducted between 2003 and 2010. These included both the Hudson and adjacent large waterways (East River, Newark Bay, Kill Van Kull and Arthur Kill) using coastal research vessels and smaller tributaries (Hackensack, Pasaic and Raritan rivers) using a 25' boat. In both cases measurements were made using towed instrument packages which could be cycled from near surface to near bottom depths with horizontal resolution of approximately 20 to 200 meters depending on depth and deployment strategy. Sensors on the instrument packages included a CTD to provide depth and salinity information and a chromophoric dissolved organic matter(CDOM) fluorometer to measure the fluorescent fraction of the DOC. Discrete samples allowed calibration of the fluorometer and the CDOM data to be related to DOC. The combined data set from these cruises identified multiple scales of source and transport processes for DOC within the Hudson River/New York Harbor region. The Hudson carries a substantial amount of natural DOC from its 230 km inland stretch. Additional sources exist in fringing salt marshes adjacent to the Hackensack and Raritan rivers. However the lower Hudson/New Harbor region receives a large input of DOC from multiple publically owned treatment works (POTW) discharges. The high resolution surveys allowed us to elucidate the distribution of these sources and the manner in which they are rapidly mixed to create the total export. We estimate that anthropogenic sources account for up to 2.5 times the DOC flux contributed by natural processes.

Isolation, screening, and characterization of surface-active agent-producing, oil-degrading marine bacteria of Mumbai Harbor.

Science.gov (United States)

Mohanram, Rajamani; Jagtap, Chandrakant; Kumar, Pradeep

2016-04-15

Diverse marine bacterial species predominantly found in oil-polluted seawater produce diverse surface-active agents. Surface-active agents produced by bacteria are classified into two groups based on their molecular weights, namely biosurfactants and bioemulsifiers. In this study, surface-active agent-producing, oil-degrading marine bacteria were isolated using a modified Bushnell-Haas medium with high-speed diesel as a carbon source from three oil-polluted sites of Mumbai Harbor. Surface-active agent-producing bacterial strains were screened using nine widely used methods. The nineteen bacterial strains showed positive results for more than four surface-active agent screening methods; further, these strains were characterized using biochemical and nucleic acid sequencing methods. Based on the results, the organisms belonged to the genera Acinetobacter, Alcanivorax, Bacillus, Comamonas, Chryseomicrobium, Halomonas, Marinobacter, Nesterenkonia, Pseudomonas, and Serratia. The present study confirmed the prevalence of surface-active agent-producing bacteria in the oil-polluted waters of Mumbai Harbor. Copyright © 2016 Elsevier Ltd. All rights reserved.

On enhancing on-line collaboration using fuzzy logic modeling

Directory of Open Access Journals (Sweden)

Leontios J. Hadjileontiadis

2004-04-01

Full Text Available Web-based collaboration calls for professional skills and competences to the benefit of the quality of the collaboration and its output. Within this framework, educational virtual environments may provide a means for training upon these skills and in particular the collaborative ones. On the basis of the existing technological means such training may be enhanced even more. Designing considerations towards this direction include the close follow-up of the collaborative activity and provision of support grounded upon a pedagogical background. To this vein, a fuzzy logic-based expert system, namely Collaboration/Reflection-Fuzzy Inference System (C/R-FIS, is presented in this paper. By means of interconnected FISs, the C/R-FIS expert system automatically evaluates the collaborative activity of two peers, during their asynchronous, written, web-based collaboration. This information is used for the provision of adaptive support to peers during their collaboration, towards equilibrium of their collaborative activity. In particular, this enhanced formative feedback aims at diminishing the possible dissonance between the individual collaborative skills by challenging self-adjustment procedures. The proposed model extents the evaluation system of a web-based collaborative tool namely Lin2k, which has served as a test-bed for the C/R-FIS experimental use. Results from its experimental use have proved the potentiality of the proposed model to significantly contribute to the enhancement of the collaborative activity and its transferability to other collaborative learning contexts, such as medicine, environmental engineering, law, and music education.

Characteristics of trophic transfer of polychlorinated biphenyls in marine organisms in Incheon North Harbor, Korea.

Science.gov (United States)

Kim, Seung Kyu; Lee, Dong Soo; Oh, Jae Ryong

2002-04-01

The trophic transfer of polychlorinated biphenyls (PCBs) was characterized for zooplankton (primarily Paracalanus spp. and Acartia spp.), pacific oyster (Crassostrea gigas), shore crab (Hemigrapsus penicillatus), and goby (Acanthogobius hasta) in the aquatic system of Incheon North Harbor, Korea. The congener pattern in the species was clearly divided by the main PCB uptake route. Compared with zooplankton and oyster, the fraction of heavier homologues increased in crab and goby that take PCBs from food. Linear relationships were observed between log (fugacity in lipid/fugacity in seawater) and log Kow for all the species. For zooplankton and oyster, such an observation should not be regarded as a true absence of superhydrophobicity, because establishment of equilibrium with seawater was not evident. For crab and goby, the absence of superhydrophobicity was evidenced by the trophic transfer factor that continuously increased with Kow up to 10(7.8). These results suggest that superhydrophobicity might be species specific. The trophic transfer factors and the fugacity levels in the lipid phase indicated that bioaccumulation in crab and goby advanced beyond the level in equilibrium with seawater in the harbor basin.

West Nile Flavivirus Polioencephalomyelitis in a harbor seal (Phoca vitulina).

Science.gov (United States)

Del Piero, F; Stremme, D W; Habecker, P L; Cantile, C

2006-01-01

A 12-year-old male harbor seal presented with progressive signs of neurologic dysfunction including head tremors, muzzle twitching, clonic spasms, and weakness. Lesions included polioencephalomyelitis with glial nodules, spheroids, neuronophagia, ring hemorrhages, and a few neutrophils. Neurons, fibers, and glial nodules were multifocally colonized with intracytoplasmic West Nile flavivirus antigens that were demonstrated using indirect immunohistochemical analysis. Flavivirus on cultured cells also was isolated and was identified by use of monoclonal antibodies and reverse transcriptase-polymerase chain reaction analysis. Clinical signs of disease and lesion morphology and distribution were similar to those of equine West Nile virus infection. Similar to horses, alpacas, humans, dogs, and reptiles, seals can be dead-end hosts of West Nile virus.

The biological assessment of flora and fauna as standards for changes in the near-shore ocean environment: a study of Barbers Point Harbor.

Science.gov (United States)

Hokama, Y; Wachi, K M; Shiraki, A; Goo, C; Ebesu, J S

2001-02-01

The biological assessments of the flora and fauna in the near-shore ocean environment, specifically Barbers Point Harbor (BPH), demonstrate the usefulness of these biological analyses for evaluation of the changes occurring following man-made excavation for expansion of the harbor. The study included identification and enumeration of macroalgae and dinoflagellates and analyses of herbivores and carnivores in four areas within the perimeter of the harbor and the north and south entrances into the harbor. Numbers of macroalgae varied between 1994 and 1999 surveys, with significant decrease in numbers in stations C, D and E. Stations A and B were similar between 1994 and 1999 with a slight increase in 1999. The significant differences were shown with the appearance of Gambierdiscus toxicus (G toxicus) in 1999 among the algae in stations A and B. Assessment of herbivores and carnivores with the immunological membrane immunobead assay using monoclonal antibody to ciguatoxin and related polyethers demonstrated an increase in fish toxicity among the herbivore from 1994-1999 (22% increase) with a decrease (22%) in non-toxic fish. This was also demonstrated in the carnivores, but to a lesser degree. It is suggested that the biological analyses of the flora and the fauna of the near-shore ocean environment are appropriate to assess the changes that occur from natural and man-made alterations.

Blockade of the ERK pathway enhances the therapeutic efficacy of the histone deacetylase inhibitor MS-275 in human tumor xenograft models

Energy Technology Data Exchange (ETDEWEB)

Sakamoto, Toshiaki; Ozaki, Kei-ichi; Fujio, Kohsuke; Kajikawa, Shu-hei [Laboratory of Cell Regulation, Department of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8521 (Japan); Uesato, Shin-ichi [Department of Biotechnology, Faculty of Engineering, Kansai University, Osaka 564-8680 (Japan); Watanabe, Kazushi [Proubase Technology Inc., Kanagawa 211-0063 (Japan); Tanimura, Susumu [Laboratory of Cell Regulation, Department of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8521 (Japan); Koji, Takehiko [Department of Histology and Cell Biology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8523 (Japan); Kohno, Michiaki, E-mail: kohnom@nagasaki-u.ac.jp [Laboratory of Cell Regulation, Department of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8521 (Japan); Proubase Technology Inc., Kanagawa 211-0063 (Japan); Kyoto University Graduate School of Pharmaceutical Sciences, Kyoto 606-8501 (Japan)

2013-04-19

Highlights: •Blockade of the ERK pathway enhances the anticancer efficacy of HDAC inhibitors. •MEK inhibitors sensitize human tumor xenografts to HDAC inhibitor cytotoxicity. •Such the enhanced efficacy is achieved by a transient blockade of the ERK pathway. •This drug combination provides a promising therapeutic strategy for cancer patients. -- Abstract: The ERK pathway is up-regulated in various human cancers and represents a prime target for mechanism-based approaches to cancer treatment. Specific blockade of the ERK pathway alone induces mostly cytostatic rather than pro-apoptotic effects, however, resulting in a limited therapeutic efficacy of the ERK kinase (MEK) inhibitors. We previously showed that MEK inhibitors markedly enhance the ability of histone deacetylase (HDAC) inhibitors to induce apoptosis in tumor cells with constitutive ERK pathway activation in vitro. To evaluate the therapeutic efficacy of such drug combinations, we administered the MEK inhibitor PD184352 or AZD6244 together with the HDAC inhibitor MS-275 in nude mice harboring HT-29 or H1650 xenografts. Co-administration of the MEK inhibitor markedly sensitized the human xenografts to MS-275 cytotoxicity. A dose of MS-275 that alone showed only moderate cytotoxicity thus suppressed the growth of tumor xenografts almost completely as well as induced a marked reduction in tumor cellularity when administered with PD184352 or AZD6244. The combination of the two types of inhibitor also induced marked oxidative stress, which appeared to result in DNA damage and massive cell death, specifically in the tumor xenografts. The enhanced therapeutic efficacy of the drug combination was achieved by a relatively transient blockade of the ERK pathway. Administration of both MEK and HDAC inhibitors represents a promising chemotherapeutic strategy with improved safety for cancer patients.

40 CFR 81.73 - South Bend-Elkhart (Indiana)-Benton Harbor (Michigan) Interstate Air Quality Control Region.

Science.gov (United States)

2010-07-01

... 40 Protection of Environment 17 2010-07-01 2010-07-01 false South Bend-Elkhart (Indiana)-Benton Harbor (Michigan) Interstate Air Quality Control Region. 81.73 Section 81.73 Protection of Environment... PLANNING PURPOSES Designation of Air Quality Control Regions § 81.73 South Bend-Elkhart (Indiana)-Benton...

The isolation and partial characterization of a highly pathogenic herpesvirus from the harbor seal (Phoca vitulina).

NARCIS (Netherlands)

A.D.M.E. Osterhaus (Albert); H. Yang (Hong); H.E.M. Spijkers (Ine); J. Groen (Jan); J.S. Teppema; G. van Steenis (Bert)

1985-01-01

textabstractThis report describes the first isolation and partial characterization of a herpesvirus from the harbor seal (Phoca vitulina). The virus was isolated during a disease outbreak in a group of young seals nursed in a seal orphanage in The Netherlands. Almost half of the seals died with

77 FR 42640 - Safety Zone; Can-Am Festival Fireworks, Black River Bay, Sackets Harbor, NY

Science.gov (United States)

2012-07-20

...-AA00 Safety Zone; Can-Am Festival Fireworks, Black River Bay, Sackets Harbor, NY AGENCY: Coast Guard... Black River Bay during the Can-Am Festival Fireworks display. This temporary safety zone is necessary to... Can-Am Festival Fireworks. This zone will be effective and enforced from 9:15 p.m. until 10:45 p.m. on...

Environment-Dependent Distribution of the Sediment nifH-Harboring Microbiota in the Northern South China Sea

Science.gov (United States)

Yang, Jinying; Li, Jing; Luan, Xiwu; Zhang, Yunbo; Gu, Guizhou; Xue, Rongrong; Zong, Mingyue; Klotz, Martin G.

2013-01-01

The South China Sea (SCS), the largest marginal sea in the Western Pacific Ocean, is a huge oligotrophic water body with very limited influx of nitrogenous nutrients. This suggests that sediment microbial N2 fixation plays an important role in the production of bioavailable nitrogen. To test the molecular underpinning of this hypothesis, the diversity, abundance, biogeographical distribution, and community structure of the sediment diazotrophic microbiota were investigated at 12 sampling sites, including estuarine, coastal, offshore, deep-sea, and methane hydrate reservoirs or their prospective areas by targeting nifH and some other functional biomarker genes. Diverse and novel nifH sequences were obtained, significantly extending the evolutionary complexity of extant nifH genes. Statistical analyses indicate that sediment in situ temperature is the most significant environmental factor influencing the abundance, community structure, and spatial distribution of the sediment nifH-harboring microbial assemblages in the northern SCS (nSCS). The significantly positive correlation of the sediment pore water NH4+ concentration with the nifH gene abundance suggests that the nSCS sediment nifH-harboring microbiota is active in N2 fixation and NH4+ production. Several other environmental factors, including sediment pore water PO43− concentration, sediment organic carbon, nitrogen and phosphorus levels, etc., are also important in influencing the community structure, spatial distribution, or abundance of the nifH-harboring microbial assemblages. We also confirmed that the nifH genes encoded by archaeal diazotrophs in the ANME-2c subgroup occur exclusively in the deep-sea methane seep areas, providing for the possibility to develop ANME-2c nifH genes as a diagnostic tool for deep-sea methane hydrate reservoir discovery. PMID:23064334

Substantial nitrous oxide emissions from intertidal sediments and groundwater in anthropogenically-impacted West Falmouth Harbor, Massachusetts

Science.gov (United States)

Moseman-Valtierra, Serena; Kroeger, Kevin D.; Crusius, John; Baldwin, Sandy; Green, Adrian; Brooks, Thomas W.; Pugh, E.

2015-01-01

Large N2O emissions were observed from intertidal sediments in a coastal estuary, West Falmouth Harbor, MA, USA. Average N2O emission rates from 41 chambers during summer 2008 were 10.7 mol N2O m(-2) h(-1)±4.43 μmol N2O m(-2) h(-1) (standard error). Emissions were highest from sediments within a known wastewater plume, where a maximum N2O emission rate was 155 μmol N2O m(-2) h(-1). Intertidal N2O fluxes were positively related to porewater ammonium concentrations at 10 and 25 cm depths. In groundwater from 7 shoreline wells, dissolved N2O ranged from 488% of saturation (56 nM N2O) to more than 13000% of saturation (1529 nM N2O) and was positively related to nitrate concentrations. Fresh and brackish porewater underlying 14 chambers was also supersaturated in N2O, ranging from 2980% to 13175% of saturation. These observations support a relationship between anthropogenic nutrient loading and N2O emissions in West Falmouth Harbor, with both groundwater sources and also local N2O production within nutrient-rich, intertidal sediments in the groundwater seepage face. N2O emissions from intertidal "hotspot" in this harbor, together with estimated surface water emissions, constituted 2.4% of the average overall rate of nitrogen export from the watershed to the estuary. This suggests that N2O emissions factors from coastal ecosystems may be underestimated. Since anthropogenic nutrient loading affects estuaries worldwide, quantification of N2O dynamics is warranted in other anthropogenically-impacted coastal ecosystems.

Substantial nitrous oxide emissions from intertidal sediments and groundwater in anthropogenically-impacted West Falmouth Harbor, Massachusetts.

Science.gov (United States)

Moseman-Valtierra, Serena; Kroeger, Kevin D; Crusius, John; Baldwin, Sandra; Green, Adrian; Brooks, T Wallace; Pugh, Emily

2015-01-01

Large N2O emissions were observed from intertidal sediments in a coastal estuary, West Falmouth Harbor, MA, USA. Average N2O emission rates from 41 chambers during summer 2008 were 10.7 mol N2O m(-2) h(-1)±4.43 μmol N2O m(-2) h(-1) (standard error). Emissions were highest from sediments within a known wastewater plume, where a maximum N2O emission rate was 155 μmol N2O m(-2) h(-1). Intertidal N2O fluxes were positively related to porewater ammonium concentrations at 10 and 25 cm depths. In groundwater from 7 shoreline wells, dissolved N2O ranged from 488% of saturation (56 nM N2O) to more than 13000% of saturation (1529 nM N2O) and was positively related to nitrate concentrations. Fresh and brackish porewater underlying 14 chambers was also supersaturated in N2O, ranging from 2980% to 13175% of saturation. These observations support a relationship between anthropogenic nutrient loading and N2O emissions in West Falmouth Harbor, with both groundwater sources and also local N2O production within nutrient-rich, intertidal sediments in the groundwater seepage face. N2O emissions from intertidal "hotspot" in this harbor, together with estimated surface water emissions, constituted 2.4% of the average overall rate of nitrogen export from the watershed to the estuary. This suggests that N2O emissions factors from coastal ecosystems may be underestimated. Since anthropogenic nutrient loading affects estuaries worldwide, quantification of N2O dynamics is warranted in other anthropogenically-impacted coastal ecosystems. Copyright © 2014 Elsevier Ltd. All rights reserved.

Cladophora (Chlorophyta) spp. harbor human bacterial pathogens in nearshore water of Lake Michigan

Science.gov (United States)

Ishii, S.; Yan, T.; Shively, D.A.; Byappanahalli, M.N.; Whitman, R.L.; Sadowsky, M.J.

2006-01-01

Cladophora glomerata, a macrophytic green alga, is commonly found in the Great Lakes, and significant accumulations occur along shorelines during the summer months. Recently, Cladophora has been shown to harbor high densities of the fecal indicator bacteria Escherichia coli and enterococci. Cladophora may also harbor human pathogens; however, until now, no studies to address this question have been performed. In the present study, we determined whether attachedCladophora, obtained from the Lake Michigan and Burns Ditch (Little Calumet River, Indiana) sides of a breakwater during the summers of 2004 and 2005, harbored the bacterial pathogens Shiga toxin-producing Escherichia coli (STEC),Salmonella, Shigella, and Campylobacter. The presence of potential pathogens and numbers of organisms were determined by using cultural methods and by using conventional PCR, most-probable-number PCR (MPN-PCR), and quantitative PCR (QPCR) performed with genus- and toxin-specific primers and probes. WhileShigella and STEC were detected in 100% and 25%, respectively, of the algal samples obtained near Burns Ditch in 2004, the same pathogens were not detected in samples collected in 2005. MPN-PCR and QPCR allowed enumeration of Salmonella in 40 to 80% of the ditch- and lakeside samples, respectively, and the densities were up to 1.6 × 103 cells per g Cladophora. Similarly, these PCR methods allowed enumeration of up to 5.4 × 102 Campylobacter cells/gCladophora in 60 to 100% of lake- and ditchside samples. The Campylobacterdensities were significantly higher (P fingerprint analyses indicated that genotypically identical Salmonella isolates were associated with geographically and temporally distinct Cladophora samples. However, Campylobacter isolates were genetically diverse. Since animal hosts are thought to be the primary habitat forCampylobacter and Salmonella species, our results suggest that Cladophora is a likely secondary habitat for pathogenic

Model based investigation of the potential lactate recovery using Electro-Enhanced Dialysis - Static analysis

DEFF Research Database (Denmark)

Prado Rubio, Oscar Andres; Jørgensen, Sten Bay; Jonsson, Gunnar Eigil

2011-01-01

The competitive ion transport through anion exchange membranes under current load conditions, referred to as the electro-enhanced dialysis process, is modeled and investigated through simulations. A dynamic model has been developed for simultaneous transport of multiple ions based on the Nernst......–Plank equation. This model accounts for the convective transport of the dissociated and undissociated species in the module channels, and the diffusion and migration across the boundary layers and membranes. The potential static flux enhancement is evaluated and compared to Donnan dialysis operation for lactate...

Can a GIS toolbox assess the environmental risk of oil spills? Implementation for oil facilities in harbors.

Science.gov (United States)

Valdor, Paloma F; Gómez, Aina G; Velarde, Víctor; Puente, Araceli

2016-04-01

Oil spills are one of the most widespread problems in port areas (loading/unloading of bulk liquid, fuel supply). Specific environmental risk analysis procedures for diffuse oil sources that are based on the evolution of oil in the marine environment are needed. Diffuse sources such as oil spills usually present a lack of information, which makes the use of numerical models an arduous and occasionally impossible task. For that reason, a tool that can assess the risk of oil spills in near-shore areas by using Geographical Information System (GIS) is presented. The SPILL Tool provides immediate results by automating the process without miscalculation errors. The tool was developed using the Python and ArcGIS scripting library to build a non-ambiguous geoprocessing workflow. The SPILL Tool was implemented for oil facilities at Tarragona Harbor (NE Spain) and validated showing a satisfactory correspondence (around 0.60 RSR error index) with the results obtained using a 2D calibrated oil transport numerical model. Copyright © 2016 Elsevier Ltd. All rights reserved.

Hydrodynamic modelling of a tidal delta wetland using an enhanced quasi-2D model

Science.gov (United States)

Wester, Sjoerd J.; Grimson, Rafael; Minotti, Priscilla G.; Booija, Martijn J.; Brugnach, Marcela

2018-04-01

Knowledge about the hydrological regime of wetlands is key to understand their physical and biological properties. Modelling hydrological and hydrodynamic processes within a wetland is therefore becoming increasingly important. 3D models have successfully modelled wetland dynamics but depend on very detailed bathymetry and land topography. Many 1D and 2D models of river deltas highly simplify the interaction between the river and wetland area or simply neglect the wetland area. This study proposes an enhanced quasi-2D modelling strategy that captures the interaction between river discharge and moon tides and the resulting hydrodynamics, while using the scarce data available. The water flow equations are discretised with an interconnected irregular cell scheme, in which a simplification of the 1D Saint-Venant equations is used to define the water flow between cells. The spatial structure of wetlands is based on the ecogeomorphology in complex estuarine deltas. The islands within the delta are modelled with levee cells, creek cells and an interior cell representing a shallow marsh wetland. The model is calibrated for an average year and the model performance is evaluated for another average year and additionally an extreme dry three-month period and an extreme wet three-month period. The calibration and evaluation are done based on two water level measurement stations and two discharge measurement stations, all located in the main rivers. Additional calibration is carried out with field water level measurements in a wetland area. Accurate simulations are obtained for both calibration and evaluation with high correlations between observed and simulated water levels and simulated discharges in the same order of magnitude as observed discharges. Calibration against field measurements showed that the model can successfully simulate the overflow mechanism in wetland areas. A sensitivity analysis for several wetland parameters showed that these parameters are all

76 FR 70479 - Draft Environmental Assessment and Safe Harbor Agreement for the Houston Toad Within Nine Texas...

Science.gov (United States)

2011-11-14

...] Draft Environmental Assessment and Safe Harbor Agreement for the Houston Toad Within Nine Texas Counties... of the endangered Houston toad (Bufo houstonensis) as a result of conservation actions, land...: [email protected] . Include ``Clear Lake Ecological Services Field Office draft Houston Toad...

The Sommerfeld enhancement in the scotogenic model with large electroweak scalar multiplets

Energy Technology Data Exchange (ETDEWEB)

Chowdhury, Talal Ahmed [Department of Physics, University of Dhaka, P.O. Box 1000, Dhaka (Bangladesh); Nasri, Salah, E-mail: talal@du.ac.bd, E-mail: snasri@uaeu.ac.ae [Department of Physics, UAE University, P.O. Box 17551, Al-Ain (United Arab Emirates)

2017-01-01

We investigate the Sommerfeld enhancement (SE) in the generalized scotogenic model with large electroweak multiplets. We focus on scalar dark matter (DM) candidate of the model and compare DM annihilation cross sections to WW , ZZ , γγ and γ Z at present day in the galactic halo for scalar doublet and its immediate generalization, the quartet in their respective viable regions of parameter space. We find that larger multiplet has sizable Sommerfeld enhanced annihilation cross section compared to the doublet and because of that it is more likely to be constrained by the current H.E.S.S. results and future CTA sensitivity limits.

Influenza A (H10N7) Virus Causes Respiratory Tract Disease in Harbor Seals and Ferrets

NARCIS (Netherlands)

van den Brand, Judith M A; Wohlsein, Peter; Herfst, Sander; Bodewes, Rogier; Pfankuche, Vanessa M; van de Bildt, Marco W G; Seehusen, Frauke; Puff, Christina; Richard, Mathilde; Siebert, Ursula; Lehnert, Kristina; Bestebroer, Theo; Lexmond, Pascal; Fouchier, Ron A M; Prenger-Berninghoff, Ellen; Herbst, Werner; Koopmans, Marion; Osterhaus, Albert D M E; Kuiken, Thijs; Baumgärtner, Wolfgang

2016-01-01

Avian influenza viruses sporadically cross the species barrier to mammals, including humans, in which they may cause epidemic disease. Recently such an epidemic occurred due to the emergence of avian influenza virus of the subtype H10N7 (Seal/H10N7) in harbor seals (Phoca vitulina). This epidemic

76 FR 21862 - Takes of Marine Mammals Incidental to Specified Activities; Harbor Activities Related to the...

Science.gov (United States)

2011-04-19

...; and 43 Northern elephant seals by Level B harassment only. To date, NMFS has issued eight, 1-year... elephant seals spend much of the year, generally about nine months, in the ocean. They are usually... Mariner harbor operations for one year. After addressing comments from NMFS, ULA modified its application...

77 FR 38587 - Takes of Marine Mammals Incidental to Specified Activities; Harbor Activities Related to the...

Science.gov (United States)

2012-06-28

... Northern elephant seals by Level B harassment only. To date, we have issued nine, one-year, Incidental... as far north as Alaska and as far south as Mexico. Northern elephant seals spend much of the year... numbers of marine mammals, incidental to conducting Delta Mariner harbor operations for one year. We...

78 FR 18277 - Special Local Regulation; Low Country Splash, Wando River, Cooper River, and Charleston Harbor...

Science.gov (United States)

2013-03-26

...-AA08 Special Local Regulation; Low Country Splash, Wando River, Cooper River, and Charleston Harbor... proposes to issue a special local regulation on the waters of the Wando River, Cooper River, and Charleston... Country Splash is scheduled to take place on the waters of the Wando River, Cooper River, and Charleston...

Enhanced HMAX model with feedforward feature learning for multiclass categorization

Directory of Open Access Journals (Sweden)

Yinlin eLi

2015-10-01

Full Text Available In recent years, the interdisciplinary research between neuroscience and computer vision has promoted the development in both fields. Many biologically inspired visual models are proposed, and among them, the Hierarchical Max-pooling model (HMAX is a feedforward model mimicking the structures and functions of V1 to posterior inferotemporal (PIT layer of the primate visual cortex, which could generate a series of position- and scale- invariant features. However, it could be improved with attention modulation and memory processing, which are two important properties of the primate visual cortex. Thus, in this paper, based on recent biological research on the primate visual cortex, we still mimic the first 100-150 milliseconds of visual cognition to enhance the HMAX model, which mainly focuses on the unsupervised feedforward feature learning process. The main modifications are as follows: 1 To mimic the attention modulation mechanism of V1 layer, a bottom-up saliency map is computed in the S1 layer of the HMAX model, which can support the initial feature extraction for memory processing; 2 To mimic the learning, clustering and short-term memory to long-term memory conversion abilities of V2 and IT, an unsupervised iterative clustering method is used to learn clusters with multiscale middle level patches, which are taken as long-term memory; 3 Inspired by the multiple feature encoding mode of the primate visual cortex, information including color, orientation, and spatial position are encoded in different layers of the HMAX model progressively. By adding a softmax layer at the top of the model, multiclass categorization experiments can be conducted, and the results on Caltech101 show that the enhanced model with a smaller memory size exhibits higher accuracy than the original HMAX model, and could also achieve better accuracy than other unsupervised feature learning methods in multiclass categorization task.

Enhanced HMAX model with feedforward feature learning for multiclass categorization.

Science.gov (United States)

Li, Yinlin; Wu, Wei; Zhang, Bo; Li, Fengfu

2015-01-01

In recent years, the interdisciplinary research between neuroscience and computer vision has promoted the development in both fields. Many biologically inspired visual models are proposed, and among them, the Hierarchical Max-pooling model (HMAX) is a feedforward model mimicking the structures and functions of V1 to posterior inferotemporal (PIT) layer of the primate visual cortex, which could generate a series of position- and scale- invariant features. However, it could be improved with attention modulation and memory processing, which are two important properties of the primate visual cortex. Thus, in this paper, based on recent biological research on the primate visual cortex, we still mimic the first 100-150 ms of visual cognition to enhance the HMAX model, which mainly focuses on the unsupervised feedforward feature learning process. The main modifications are as follows: (1) To mimic the attention modulation mechanism of V1 layer, a bottom-up saliency map is computed in the S1 layer of the HMAX model, which can support the initial feature extraction for memory processing; (2) To mimic the learning, clustering and short-term memory to long-term memory conversion abilities of V2 and IT, an unsupervised iterative clustering method is used to learn clusters with multiscale middle level patches, which are taken as long-term memory; (3) Inspired by the multiple feature encoding mode of the primate visual cortex, information including color, orientation, and spatial position are encoded in different layers of the HMAX model progressively. By adding a softmax layer at the top of the model, multiclass categorization experiments can be conducted, and the results on Caltech101 show that the enhanced model with a smaller memory size exhibits higher accuracy than the original HMAX model, and could also achieve better accuracy than other unsupervised feature learning methods in multiclass categorization task.

Metabolite cross-feeding enhances virulence in a model polymicrobial infection.

Directory of Open Access Journals (Sweden)

Matthew M Ramsey

2011-03-01

Full Text Available Microbes within polymicrobial infections often display synergistic interactions resulting in enhanced pathogenesis; however, the molecular mechanisms governing these interactions are not well understood. Development of model systems that allow detailed mechanistic studies of polymicrobial synergy is a critical step towards a comprehensive understanding of these infections in vivo. In this study, we used a model polymicrobial infection including the opportunistic pathogen Aggregatibacter actinomycetemcomitans and the commensal Streptococcus gordonii to examine the importance of metabolite cross-feeding for establishing co-culture infections. Our results reveal that co-culture with S. gordonii enhances the pathogenesis of A. actinomycetemcomitans in a murine abscess model of infection. Interestingly, the ability of A. actinomycetemcomitans to utilize L-lactate as an energy source is essential for these co-culture benefits. Surprisingly, inactivation of L-lactate catabolism had no impact on mono-culture growth in vitro and in vivo suggesting that A. actinomycetemcomitans L-lactate catabolism is only critical for establishing co-culture infections. These results demonstrate that metabolite cross-feeding is critical for A. actinomycetemcomitans to persist in a polymicrobial infection with S. gordonii supporting the idea that the metabolic properties of commensal bacteria alter the course of pathogenesis in polymicrobial communities.

Caffeine in an Urbanized Estuary: Past and Present Influence of Wastewater Effluents in Boston Harbor, MA, USA

Science.gov (United States)

Caffeine has been identified by previous research as a potential tracer of sanitary wastewater. To further assess the utility of caffeine as a tracer of wastewater sources, samples from 25 sites throughout Boston Harbor were collected and analyzed for caffeine by LC-MS/MS. Caff...

76 FR 20287 - Superfund Site, New Bedford Harbor, New Bedford, MA: Anchorage Ground and Regulated Navigation Area

Science.gov (United States)

2011-04-12

... may lead to the discovery of a significant environmental impact from this proposed rule. List of... engaged in activities associated with remediation efforts in the New Bedford Harbor Superfund Site... activity can be performed without undue risk to environmental remediation efforts. Requests for waivers...

75 FR 1734 - Children’s Online Privacy Protection Rule Safe Harbor Proposed Self-Regulatory Guidelines; i-SAFE...

Science.gov (United States)

2010-01-13

... Proposed Self-Regulatory Guidelines; i-SAFE, Inc. Application for Safe Harbor AGENCY: Federal Trade... for public comment concerning proposed self-regulatory guidelines submitted by i-SAFE, Inc. under the... approval self-regulatory guidelines that would implement the Rule's protections.\\3\\ \\1\\ 64 FR 59888 (1999...

Critical ratios in harbor porpoises (Phocoena phocoena) for tonal signals between 0.315 and 150 kHz in random Gaussian white noise.

Science.gov (United States)

Kastelein, Ronald A; Wensveen, Paul J; Hoek, Lean; Au, Whitlow W L; Terhune, John M; de Jong, Christ A F

2009-09-01

A psychoacoustic behavioral technique was used to determine the critical ratios (CRs) of two harbor porpoises for tonal signals with frequencies between 0.315 and 150 kHz, in random Gaussian white noise. The masked 50% detection hearing thresholds were measured using a "go/no-go" response paradigm and an up-down staircase psychometric method. CRs were determined at one masking noise level for each test frequency and were similar in both animals. For signals between 0.315 and 4 kHz, the CRs were relatively constant at around 18 dB. Between 4 and 150 kHz the CR increased gradually from 18 to 39 dB ( approximately 3.3 dB/octave). Generally harbor porpoises can detect tonal signals in Gaussian white noise slightly better than most odontocetes tested so far. By combining the mean CRs found in the present study with the spectrum level of the background noise levels at sea, the basic audiogram, and the directivity index, the detection threshold levels of harbor porpoises for tonal signals in various sea states can be calculated.

Enhanced oil recovery by nanoparticles injection: Modeling and simulation

KAUST Repository

El-Amin, Mohamed; Sun, Shuyu; Salama, Amgad

2013-01-01

In the present paper, a mathematical model and numerical simulation to describe the nanoparticles-water suspension imbibes into a water-oil two-phase flow in a porous medium is introduced. We extend the model to include the negative capillary pressure and mixed relative permeabilities correlations to fit with the mixed-wet system. Also, buoyancy and capillary forces as well as Brownian diffusion are considered. Throughout this investigation, we monitor the changing of the fluids and solid properties due to addition of the nanoparticles and check for possible enhancement of the oil recovery process using numerical experiments.

Ecological evaluation of proposed dredged material from Richmond Harbor

International Nuclear Information System (INIS)

Pinza, M.R.; Ward, J.A.; Mayhew, H.L.; Word, J.Q.; Niyogi, D.K.; Kohn, N.P.

1992-10-01

During the summer of 1991, Battelle/Marine Sciences Laboratory (MSL) was contracted to conduct sampling and testing of sediments proposed for dredging of Richmond Harbor, California. The MSL collected sediment cores to a depth of -40 ft MLLW (-38 ft + 2 ft overdepth) from 28 (12-in. core) and 30 (4-in. core) stations. The sediment cores were allocated to six composite samples referred to as sediment treatments, which were then subjected to physical, chemical, toxicological, and bioaccumulation testing. Physical and chemical parameters included grain size, total organic carbon (TOC), total volatile solids (TVS), oil and grease, total petroleum hydrocarbons (TPH), polynuclear aromatic hydrocarbons (PAH), chlorinated pesticides, polychlorinated biphenyis (PCBs), priority pollutant metals, and butyltins. The results from the test treatments were compared to results from five reference treatments representative of potential in-bay and offshore disposal sites

Ecological evaluation of proposed dredged material from Richmond Harbor

Energy Technology Data Exchange (ETDEWEB)

Pinza, M R; Ward, J A; Mayhew, H L; Word, J Q; Niyogi, D K; Kohn, N P [Battelle/Marine Sciences Lab., Sequim, WA (United States)

1992-10-01

During the summer of 1991, Battelle/Marine Sciences Laboratory (MSL) was contracted to conduct sampling and testing of sediments proposed for dredging of Richmond Harbor, California. The MSL collected sediment cores to a depth of [minus]40 ft MLLW ([minus]38 ft + 2 ft overdepth) from 28 (12-in. core) and 30 (4-in. core) stations. The sediment cores were allocated to six composite samples referred to as sediment treatments, which were then subjected to physical, chemical, toxicological, and bioaccumulation testing. Physical and chemical parameters included grain size, total organic carbon (TOC), total volatile solids (TVS), oil and grease, total petroleum hydrocarbons (TPH), polynuclear aromatic hydrocarbons (PAH), chlorinated pesticides, polychlorinated biphenyis (PCBs), priority pollutant metals, and butyltins. The results from the test treatments were compared to results from five reference treatments representative of potential in-bay and offshore disposal sites.

75 FR 38019 - Safety Zone; Fixed Mooring Balls, South of Barbers Pt. Harbor Channel, Oahu, HI

Science.gov (United States)

2010-07-01

... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 [Docket No. USCG-2010-0457] RIN 1625-AA00 Safety Zone; Fixed Mooring Balls, South of Barbers Pt. Harbor Channel, Oahu, HI AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: Due to the placement of six fixed mooring balls in an...

Establishment of atherosclerotic model and USPIO enhanced MRI techniques study in rabbits

International Nuclear Information System (INIS)

Li Yonggang; Zhu Mo; Dai Yinyu; Chen Jianhua; Guo Liang; Ni Jiankun

2010-01-01

Objective: To explore the methods of establishment of atherosclerotic model and USPIO enhanced MRI techniques in rabbits. Methods: Thirty New Zealand male rabbits were divided randomly into two groups: 20 animals in the experiment group, 10 animals in the control group. Animal model of atherosclerosis was induced with aortic balloon endothelial injury and high-fat diet feeding. There was no intervention with the rabbits in control group. MRI examination included plan scan, USPIO enhanced black-blood sequences and white-blood sequence. The features of the plaques was analyzed in the experimental group and the effection on the image quality of different coils, sequences and parameters and a statistical study was also analyzed. Results: Animal model of atherosclerosis was successfully made in 12 rabbits and most plaques located in the abdomen aorta. There were 86 plaques within the scanning scope among which 67 plaques were positive to the Prussian blue staining. The image quality of knee joint coil was better than that of other coils. Although there was no difference in the detection of numbers of AS plaques between USPIO enhanced black-blood sequences and white-blood sequence (P > 0.05), blackblood sequences was superior to white-blood sequence in the demonstration of the components of plaque. Conclusion: The method of aortic balloon endothelial injury and high-fat diet feeding can easily establish the AS model in rabbits with a shorter period and it may be used for controlling the location of the plaques. USPIO enhanced MRI sequences has high sensitivity in the detection of the AS plauqes and can reveal the component of AS plaques. The optimization of MRI techniques is very important in the improvement of the image quality and the detection of the plaques. (authors)

Geosynchronous magnetic field responses to fast solar wind dynamic pressure enhancements: MHD field model

Directory of Open Access Journals (Sweden)

T. R. Sun

2012-08-01

Full Text Available We performed global MHD simulations of the geosynchronous magnetic field in response to fast solar wind dynamic pressure (Pd enhancements. Taking three Pd enhancement events in 2000 as examples, we found that the main features of the total field B and the dominant component Bz can be efficiently predicted by the MHD model. The predicted B and Bz varies with local time, with the highest level near noon and a slightly lower level around mid-night. However, it is more challenging to accurately predict the responses of the smaller component at the geosynchronous orbit (i.e., Bx and By. In contrast, the limitations of T01 model in predicting responses to fast Pd enhancements are presented.

Nonlinear modeling of a rotational MR damper via an enhanced Bouc–Wen model

International Nuclear Information System (INIS)

Miah, Mohammad S; Chatzi, Eleni N; Dertimanis, Vasilis K; Weber, Felix

2015-01-01

The coupling of magnetorheological (MR) dampers with semi-active control schemes has proven to be an effective and failsafe approach for vibration mitigation of low-damped structures. However, due to the nonlinearities inherently relating to such damping devices, the characterization of the associated nonlinear phenomena is still a challenging task. Herein, an enhanced phenomenological modeling approach is proposed for the description of a rotational-type MR damper, which comprises a modified Bouc–Wen model coupled with an appropriately selected sigmoid function. In a first step, parameter optimization is performed on the basis of individual models in an effort to approximate the experimentally observed response for varying current levels and actuator force characteristics. In a second step, based on the previously identified parameters, a generalized best-fit model is proposed by performing a regression analysis. Finally, model validation is carried out via implementation on different sets of experimental data. The proposed model indeed renders an improved representation of the actually observed nonlinear behavior of the tested rotational MR damper. (paper)

Histone modification enhances the effectiveness of IL-13 receptor targeted immunotoxin in murine models of human pancreatic cancer

Directory of Open Access Journals (Sweden)

Puri Raj K

2011-04-01

Full Text Available Abstract Background Interleukin-13 Receptor α2 (IL-13Rα2 is a tumor-associated antigen and target for cancer therapy. Since IL-13Rα2 is heterogeneously overexpressed in a variety of human cancers, it would be highly desirable to uniformly upregulate IL-13Rα2 expression in tumors for optimal targeting. Methods We examined epigenetic regulation of IL-13Rα2 in a murine model of human pancreatic cancer by Bisulfite-PCR, sequencing for DNA methylation and chromatin immunoprecipitation for histone modification. Reverse transcription-PCR was performed for examining changes in IL-13Rα2 mRNA expression after treatment with histone deacetylase (HDAC and c-jun inhibitors. In vitro cytotoxicity assays and in vivo testing in animal tumor models were performed to determine whether HDAC inhibitors could enhance anti-tumor effects of IL-13-PE in pancreatic cancer. Mice harboring subcutaneous tumors were treated with HDAC inhibitors systemically and IL-13-PE intratumorally. Results We found that CpG sites in IL-13Rα2 promoter region were not methylated in all pancreatic cancer cell lines studied including IL-13Rα2-positive and IL-13Rα2-negative cell lines and normal cells. On the other hand, histones at IL-13Rα2 promoter region were highly-acetylated in IL-13Rα2-positive but much less in receptor-negative pancreatic cancer cell lines. When cells were treated with HDAC inhibitors, not only histone acetylation but also IL-13Rα2 expression was dramatically enhanced in receptor-negative pancreatic cancer cells. In contrast, HDAC inhibition did not increase IL-13Rα2 in normal cell lines. In addition, c-jun in IL-13Rα2-positive cells was expressed at higher level than in negative cells. Two types of c-jun inhibitors prevented increase of IL-13Rα2 by HDAC inhibitors. HDAC inhibitors dramatically sensitized cancer cells to immunotoxin in the cytotoxicity assay in vitro and increased IL-13Rα2 in the tumors subcutaneously implanted in the immunodeficient

Ground level enhancement (GLE) energy spectrum parameters model

Science.gov (United States)

Qin, G.; Wu, S.

2017-12-01

We study the ground level enhancement (GLE) events in solar cycle 23 with the four energy spectra parameters, the normalization parameter C, low-energy power-law slope γ 1, high-energy power-law slope γ 2, and break energy E0, obtained by Mewaldt et al. 2012 who fit the observations to the double power-law equation. we divide the GLEs into two groups, one with strong acceleration by interplanetary (IP) shocks and another one without strong acceleration according to the condition of solar eruptions. We next fit the four parameters with solar event conditions to get models of the parameters for the two groups of GLEs separately. So that we would establish a model of energy spectrum for GLEs for the future space weather prediction.

Functional analysis of limb transcriptional enhancers in the mouse.

Science.gov (United States)

Nolte, Mark J; Wang, Ying; Deng, Jian Min; Swinton, Paul G; Wei, Caimiao; Guindani, Michele; Schwartz, Robert J; Behringer, Richard R

2014-01-01

Transcriptional enhancers are genomic sequences bound by transcription factors that act together with basal transcriptional machinery to regulate gene transcription. Several high-throughput methods have generated large datasets of tissue-specific enhancer sequences with putative roles in developmental processes. However, few enhancers have been deleted from the genome to determine their roles in development. To understand the roles of two enhancers active in the mouse embryonic limb bud we deleted them from the genome. Although the genes regulated by these enhancers are unknown, they were selected because they were identified in a screen for putative limb bud-specific enhancers associated with p300, an acetyltransferase that participates in protein complexes that promote active transcription, and because the orthologous human enhancers (H1442 and H280) drive distinct lacZ expression patterns in limb buds of embryonic day (E) 11.5 transgenic mice. We show that the orthologous mouse sequences, M1442 and M280, regulate dynamic expression in the developing limb. Although significant transcriptional differences in enhancer-proximal genes in embryonic limb buds accompany the deletion of M1442 and M280 no gross limb malformations during embryonic development were observed, demonstrating that M1442 and M280 are not required for mouse limb development. However, M280 is required for the development and/or maintenance of body size; M280 mice are significantly smaller than controls. M280 also harbors an "ultraconserved" sequence that is identical between human, rat, and mouse. This is the first report of a phenotype resulting from the deletion of an ultraconserved element. These studies highlight the importance of determining enhancer regulatory function by experiments that manipulate them in situ and suggest that some of an enhancer's regulatory capacities may be developmentally tolerated rather than developmentally required. © 2014 Wiley Periodicals, Inc.

The potential of chitosan in enhancing peptide and protein absorption across the TR146 cell culture model-an in vitro model of the buccal epithelium

DEFF Research Database (Denmark)

Portero, Ana; Remuñán-López, Carmen; Nielsen, Hanne Mørck

2002-01-01

To investigate the potential of chitosan (CS) to enhance buccal peptide and protein absorption, the TR146 cell culture model, a model of the buccal epithelium, was used.......To investigate the potential of chitosan (CS) to enhance buccal peptide and protein absorption, the TR146 cell culture model, a model of the buccal epithelium, was used....

Implementing Outcome Measures Within an Enhanced Palliative Care Day Care Model.

LENUS (Irish Health Repository)

Kilonzo, Isae

2015-04-23

Specialist palliative care day care (SPDC) units provide an array of services to patients and their families and can increase continuity of care between inpatient and homecare settings. A multidisciplinary teamwork approach is emphasized, and different models of day care exist. Depending on the emphasis of care, the models can be social, medical, therapeutic, or mixed. We describe our experience of introducing an enhanced therapeutic specialist day care model and using both patient- and carer-rated tools to monitor patient outcomes.

The enhancement of mathematical analogical reasoning ability of university students through concept attainment model

Science.gov (United States)

Angraini, L. M.; Kusumah, Y. S.; Dahlan, J. A.

2018-05-01

This study aims to see the enhancement of mathematical analogical reasoning ability of the university students through concept attainment model learning based on overall and Prior Mathematical Knowledge (PMK) and interaction of both. Quasi experiments with the design of this experimental-controlled equivalent group involved 54 of second semester students at the one of State Islamic University. The instrument used is pretest-postest. Kolmogorov-Smirnov test, Levene test, t test, two-way ANOVA test were used to analyse the data. The result of this study includes: (1) The enhancement of the mathematical analogical reasoning ability of the students who gets the learning of concept attainment model is better than the enhancement of the mathematical analogical reasoning ability of the students who gets the conventional learning as a whole and based on PMK; (2) There is no interaction between the learning that is used and PMK on enhancing mathematical analogical reasoning ability.

2014 Enhanced LAW Glass Property-Composition Models, Phase 2

Energy Technology Data Exchange (ETDEWEB)

Muller, Isabelle [The Catholic Univ. of America, Washington, DC (United States); Pegg, Ian L. [The Catholic Univ. of America, Washington, DC (United States); Joseph, Innocent [Energy Solutions, Salt Lake City, UT (United States); Gilbo, Konstantin [The Catholic Univ. of America, Washington, DC (United States)

2015-10-28

This report describes the results of testing specified by the Enhanced LAW Glass Property-Composition Models, VSL-13T3050-1, Rev. 0 Test Plan. The work was performed in compliance with the quality assurance requirements specified in the Test Plan. Results required by the Test Plan are reported. The te4st results and this report have been reviewed for correctness, technical adequacy, completeness, and accuracy.

The effect of a large Danish offshore wind farm on harbor and grey seal haul-out behaviour

DEFF Research Database (Denmark)

Edrén, Susi Manuela Clermont; Andersen, Signe May; Teilmann, Jonas

2010-01-01

This study investigates the effects of the construction and operation of a large Danish offshore wind farm on harbor and gray seal haul-out behavior within a nearby (4 km) seal sanctuary. Time-lapse photography, visual monitoring, and aerial surveys were used to monitor the number of seals on land...... in daylight hours. Seals were monitored during two preconstruction periods (19 June–31 August 2001 and April–August 2002), a construction period of the wind farm (August 2002–December 2003), and a period of operation of the wind farm (December 2003–December 2004). Monthly aerial surveys were conducted...... to estimate the proportion of seals in the sanctuary relative to neighboring haul-out sites. From preconstruction to construction and through the first year of operation the number of harbor seals in the sanctuary increased at the same rate as the number of seals at the neighboring haul-out sites. No long...

Testing the Two-Layer Model for Correcting Near Cloud Reflectance Enhancement Using LES SHDOM Simulated Radiances

Science.gov (United States)

Wen, Guoyong; Marshak, Alexander; Varnai, Tamas; Levy, Robert

2016-01-01

A transition zone exists between cloudy skies and clear sky; such that, clouds scatter solar radiation into clear-sky regions. From a satellite perspective, it appears that clouds enhance the radiation nearby. We seek a simple method to estimate this enhancement, since it is so computationally expensive to account for all three-dimensional (3-D) scattering processes. In previous studies, we developed a simple two-layer model (2LM) that estimated the radiation scattered via cloud-molecular interactions. Here we have developed a new model to account for cloud-surface interaction (CSI). We test the models by comparing to calculations provided by full 3-D radiative transfer simulations of realistic cloud scenes. For these scenes, the Moderate Resolution Imaging Spectroradiometer (MODIS)-like radiance fields were computed from the Spherical Harmonic Discrete Ordinate Method (SHDOM), based on a large number of cumulus fields simulated by the University of California, Los Angeles (UCLA) large eddy simulation (LES) model. We find that the original 2LM model that estimates cloud-air molecule interactions accounts for 64 of the total reflectance enhancement and the new model (2LM+CSI) that also includes cloud-surface interactions accounts for nearly 80. We discuss the possibility of accounting for cloud-aerosol radiative interactions in 3-D cloud-induced reflectance enhancement, which may explain the remaining 20 of enhancements. Because these are simple models, these corrections can be applied to global satellite observations (e.g., MODIS) and help to reduce biases in aerosol and other clear-sky retrievals.

Colorimetric DNAzyme Biosensor for Convenience Detection of Enterotoxin B Harboring Staphylococcus aureus from Food Samples.

Science.gov (United States)

Mondal, Bhairab; N, Bhavanashri; Ramlal, Shylaja; Kingston, Joseph

2018-02-14

In the present study, a colorimetric DNAzymes biosensor strategy was devised in combination with immunomagnetic separation for rapid and easy detection of enterotoxin B harboring Staphylococcus aureus from food and clinical samples. The method employs immunocapture of S. aureus and amplification of seb gene by DNAzyme complementary sequence integrated forward primer and with specific reverse primer. The DNAzyme sequence integrated dsDNA PCR products when treated with hemin and TMB (3,3',5,5'-tetramethylbenzidine) in the presence of H 2 O 2 produce colorimetric signal. A linear relationship of optical signal with the initial template of seb was obtained which could be monitored by visually or spectrophotrometrically for qualitative and quantitative detection. The limit of detection for the assay was approximately 10 2 CFU/mL of seb gene harboring target. This method is convenient compared to gel based and ELISA systems. Further, spiking studies and analysis on natural samples emphasized the robustness and applicability of developed method. Altogether, the established assay could be a reliable alternative, low-cost, viable detection tool for the routine investigation of seb from food and clinical sources.

A Decision Mixture Model-Based Method for Inshore Ship Detection Using High-Resolution Remote Sensing Images.

Science.gov (United States)

Bi, Fukun; Chen, Jing; Zhuang, Yin; Bian, Mingming; Zhang, Qingjun

2017-06-22

With the rapid development of optical remote sensing satellites, ship detection and identification based on large-scale remote sensing images has become a significant maritime research topic. Compared with traditional ocean-going vessel detection, inshore ship detection has received increasing attention in harbor dynamic surveillance and maritime management. However, because the harbor environment is complex, gray information and texture features between docked ships and their connected dock regions are indistinguishable, most of the popular detection methods are limited by their calculation efficiency and detection accuracy. In this paper, a novel hierarchical method that combines an efficient candidate scanning strategy and an accurate candidate identification mixture model is presented for inshore ship detection in complex harbor areas. First, in the candidate region extraction phase, an omnidirectional intersected two-dimension scanning (OITDS) strategy is designed to rapidly extract candidate regions from the land-water segmented images. In the candidate region identification phase, a decision mixture model (DMM) is proposed to identify real ships from candidate objects. Specifically, to improve the robustness regarding the diversity of ships, a deformable part model (DPM) was employed to train a key part sub-model and a whole ship sub-model. Furthermore, to improve the identification accuracy, a surrounding correlation context sub-model is built. Finally, to increase the accuracy of candidate region identification, these three sub-models are integrated into the proposed DMM. Experiments were performed on numerous large-scale harbor remote sensing images, and the results showed that the proposed method has high detection accuracy and rapid computational efficiency.

78 FR 79201 - Medicare and State Health Care Programs: Fraud and Abuse; Electronic Health Records Safe Harbor...

Science.gov (United States)

2013-12-27

... limitation, kickbacks, bribes, and rebates, whether made directly or indirectly, overtly or covertly, in cash... the purchasing, leasing, or ordering of, or arranging for or recommending the purchasing, leasing, or... harbor at 42 CFR 1001.952(y). Arrangements that disguise the ``purchase'' or lock-in of referrals and...

Threshold received sound pressure levels of single 1-2 kHz and 6-7 kHz up-sweeps and down-sweeps causing startle responses in a harbor porpoise (Phocoena phocoena)

NARCIS (Netherlands)

Kastelein, R.A.; Steen, N.; Gransier, R.; Wensveen, P.J.; Jong, C.A.F. de

2012-01-01

Mid-frequency and low-frequency sonar systems produce frequency-modulated sweeps which may affect harbor porpoises. To study the effect of sweeps on behavioral responses (specifically startle responses, which we define as sudden changes in swimming speed and/or direction), a harbor porpoise in a

Development and Validation of an Enhanced Coupled-Field Model for PZT Cantilever Bimorph Energy Harvester

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Long Zhang

2013-01-01

Full Text Available The power source with the limited life span has motivated the development of the energy harvesters that can scavenge the ambient environment energy and convert it into the electrical energy. With the coupled field characteristics of structure to electricity, piezoelectric energy harvesters are under consideration as a means of converting the mechanical energy to the electrical energy, with the goal of realizing completely self-powered sensor systems. In this paper, two previous models in the literatures for predicting the open-circuit and close-circuit voltages of a piezoelectric cantilever bimorph (PCB energy harvester are first described, that is, the mechanical equivalent spring mass-damper model and the electrical equivalent circuit model. Then, the development of an enhanced coupled field model for the PCB energy harvester based on another previous model in the literature using a conservation of energy method is presented. Further, the laboratory experiments are carried out to evaluate the enhanced coupled field model and the other two previous models in the literatures. The comparison results show that the enhanced coupled field model can better predict the open-circuit and close-circuit voltages of the PCB energy harvester with a proof mass bonded at the free end of the structure in order to increase the energy-harvesting level of the system.

Geographic, seasonal, and diurnal surface behavior of harbor porpoises

DEFF Research Database (Denmark)

Teilmann, Jonas; Christiansen, C.T.; Kjellerup, Sanne

2013-01-01

are essential information on the status and management of the species. Thirty-five free-ranging harbor porpoises (Phocoena phocoena) were tracked in the region between the Baltic and the North Sea for 25-349 d using Argos satellite transmitters. No differences were found in surface behavior between geographical...... areas or the size of the animals. Slight differences were found between the two sexes and time of day. Surface time peaked in April, where 6% was spent with the transmitter above surface and 61.5% between 0 and 2 m depth, while the minimum values occurred in February (3.4% and 42.5%, respectively......). The analyses reveal that individual variation among porpoises is the most important factor in explaining variation in surface rates. However, the large number of animals documented in the present study covering a wide range of age and sex groups justifies the use of the seasonal average surface times...

Modeling of Coastal Effluent Transport: an Approach to Linking of Far-field and Near-field Models

International Nuclear Information System (INIS)

Yang, Zhaoqing; Khangaonkar, Tarang P.

2008-01-01

One of the challenges in effluent transport modeling in coastal tidal environments is the proper calculation of initial dilution in connection with the far-field transport model. In this study, an approach of external linkage of far-field and near-field effluent transport models is presented, and applied to simulate the effluent transport in the Port Angeles Harbor, Washington in the Strait of Juan de Fuca. A near-field plume model was used to calculate the effluent initial dilution and a three-dimensional (3-D) hydrodynamic model was developed to simulate the tidal circulation and far-field effluent transport in the Port Angeles Harbor. In the present study, the hydrodynamic model was driven by tides and surface winds. Observed water surface elevation and velocity data were used to calibrate the model over a period covering the neap-spring tidal cycle. The model was also validated with observed surface drogue trajectory data. The model successfully reproduced the tidal dynamics in the study area and good agreements between model results and observed data were obtained. This study demonstrated that the linkage between the near-field and far-field models in effluent transport modeling can be achieved through iteratively adjusting the model grid sizes such that the far-field modeled dilution ratio and effluent concentration in the effluent discharge model grid cell match the concentration calculated by the near-field plume model

A Comprehensive Model of Electric-Field-Enhanced Jumping-Droplet Condensation on Superhydrophobic Surfaces.

Science.gov (United States)

Birbarah, Patrick; Li, Zhaoer; Pauls, Alexander; Miljkovic, Nenad

2015-07-21

Superhydrophobic micro/nanostructured surfaces for dropwise condensation have recently received significant attention due to their potential to enhance heat transfer performance by shedding positively charged water droplets via coalescence-induced droplet jumping at length scales below the capillary length and allowing the use of external electric fields to enhance droplet removal and heat transfer, in what has been termed electric-field-enhanced (EFE) jumping-droplet condensation. However, achieving optimal EFE conditions for enhanced heat transfer requires capturing the details of transport processes that is currently lacking. While a comprehensive model has been developed for condensation on micro/nanostructured surfaces, it cannot be applied for EFE condensation due to the dynamic droplet-vapor-electric field interactions. In this work, we developed a comprehensive physical model for EFE condensation on superhydrophobic surfaces by incorporating individual droplet motion, electrode geometry, jumping frequency, field strength, and condensate vapor-flow dynamics. As a first step toward our model, we simulated jumping droplet motion with no external electric field and validated our theoretical droplet trajectories to experimentally obtained trajectories, showing excellent temporal and spatial agreement. We then incorporated the external electric field into our model and considered the effects of jumping droplet size, electrode size and geometry, condensation heat flux, and droplet jumping direction. Our model suggests that smaller jumping droplet sizes and condensation heat fluxes require less work input to be removed by the external fields. Furthermore, the results suggest that EFE electrodes can be optimized such that the work input is minimized depending on the condensation heat flux. To analyze overall efficiency, we defined an incremental coefficient of performance and showed that it is very high (∼10(6)) for EFE condensation. We finally proposed mechanisms

78 FR 11116 - Safety Zone; Mamaroneck Beach and Yacht Club Fireworks, Mamaroneck Harbor, Long Island Sound, NY

Science.gov (United States)

2013-02-15

... 1625-AA00 Safety Zone; Mamaroneck Beach and Yacht Club Fireworks, Mamaroneck Harbor, Long Island Sound... to establish a temporary safety zone on the navigable waters of Long Island Sound in the vicinity of... from a portion of Long Island Sound before, during, and immediately after the fireworks event. DATES...

HIV-1 replication in cell lines harboring INI1/hSNF5 mutations

Directory of Open Access Journals (Sweden)

Wu Xuhong

2006-08-01

Full Text Available Abstract Background INI1/hSNF5 is a cellular protein that directly interacts with HIV-1 integrase (IN. It is specifically incorporated into HIV-1 virions. A dominant negative mutant derived from INI1 inhibits HIV-1 replication. Recent studies indicate that INI1 is associated with pre-integration and reverse transcription complexes that are formed upon viral entry into the target cells. INI1 also is a tumor suppressor, biallelically deleted/mutated in malignant rhabdoid tumors. We have utilized cell lines derived from the rhabdoid tumors, MON and STA-WT1, that harbor either null or truncating mutations of INI1 respectively, to assess the effect of INI1 on HIV-1 replication. Results We found that while HIV-1 virions produced in 293T cells efficiently transduced MON and STA-WT1 cells, HIV-1 particle production was severely reduced in both of these cells. Reintroduction of INI1 into MON and STA-WT1 significantly enhanced the particle production in both cell lines. HIV-1 particles produced in MON cells were reduced for infectivity, while those produced in STA-WT1 were not. Further analysis indicated the presence of INI1 in those virions produced from STA-WT1 but not from those produced from MON cells. HIV-1 produced in MON cells were defective for synthesis of early and late reverse transcription products in the target cells. Furthermore, virions produced in MON cells were defective for exogenous reverse transcriptase activity carried out using exogenous template, primer and substrate. Conclusion Our results suggest that INI1-deficient cells exhibit reduced particle production that can be partly enhanced by re-introduction of INI1. Infectivity of HIV-1 produced in some but not all INI1 defective cells, is affected and this defect may correlate to the lack of INI1 and/or some other proteins in these virions. The block in early events of virion produced from MON cells appears to be at the stage of reverse transcription. These studies suggest that

HIV-1 replication in cell lines harboring INI1/hSNF5 mutations.

Science.gov (United States)

Sorin, Masha; Yung, Eric; Wu, Xuhong; Kalpana, Ganjam V

2006-08-31

INI1/hSNF5 is a cellular protein that directly interacts with HIV-1 integrase (IN). It is specifically incorporated into HIV-1 virions. A dominant negative mutant derived from INI1 inhibits HIV-1 replication. Recent studies indicate that INI1 is associated with pre-integration and reverse transcription complexes that are formed upon viral entry into the target cells. INI1 also is a tumor suppressor, biallelically deleted/mutated in malignant rhabdoid tumors. We have utilized cell lines derived from the rhabdoid tumors, MON and STA-WT1, that harbor either null or truncating mutations of INI1 respectively, to assess the effect of INI1 on HIV-1 replication. We found that while HIV-1 virions produced in 293T cells efficiently transduced MON and STA-WT1 cells, HIV-1 particle production was severely reduced in both of these cells. Reintroduction of INI1 into MON and STA-WT1 significantly enhanced the particle production in both cell lines. HIV-1 particles produced in MON cells were reduced for infectivity, while those produced in STA-WT1 were not. Further analysis indicated the presence of INI1 in those virions produced from STA-WT1 but not from those produced from MON cells. HIV-1 produced in MON cells were defective for synthesis of early and late reverse transcription products in the target cells. Furthermore, virions produced in MON cells were defective for exogenous reverse transcriptase activity carried out using exogenous template, primer and substrate. Our results suggest that INI1-deficient cells exhibit reduced particle production that can be partly enhanced by re-introduction of INI1. Infectivity of HIV-1 produced in some but not all INI1 defective cells, is affected and this defect may correlate to the lack of INI1 and/or some other proteins in these virions. The block in early events of virion produced from MON cells appears to be at the stage of reverse transcription. These studies suggest that presence of INI1 or some other host factor in virions and

Impact of enhanced ozone deposition and halogen chemistry on model performance

Science.gov (United States)

In this study, an enhanced ozone deposition scheme due to the interaction of iodide in sea-water and atmospheric ozone and the detailed chemical reactions of organic and inorganic halogen species are incorporated into the hemispheric Community Multiscale Air Quality model. Prelim...

COMPARISON OF ALPHA-ELEMENT-ENHANCED SIMPLE STELLAR POPULATION MODELS WITH MILKY WAY GLOBULAR CLUSTERS

International Nuclear Information System (INIS)

Lee, Hyun-chul; Worthey, Guy; Dotter, Aaron

2009-01-01

We present simple stellar population (SSP) models with scaled-solar and α-element-enhanced abundances. The SSP models are based on the Dartmouth Stellar Evolution Database, our library of synthetic stellar spectra, and a detailed systematic variation of horizontal-branch (HB) morphology with age and metallicity. In order to test the relative importance of a variety of SSP model ingredients, we compare our SSP models with integrated spectra of 41 Milky Way globular clusters (MWGCs) from Schiavon et al. Using the Mg b and Ca4227 indices, we confirm that Mg and Ca are enhanced by about +0.4 and +0.2 dex, respectively, in agreement with results from high-resolution spectra of individual stars in MWGCs. Balmer lines, particularly Hγ and Hδ, of MWGCs are reproduced by our α-enhanced SSP models not only because of the combination of isochrone and spectral effects but also because of our reasonable HB treatment. Moreover, it is shown that the Mg abundance significantly influences Balmer and iron line indices. Finally, the investigation of power-law initial mass function (IMF) variations suggests that an IMF much shallower than Salpeter is unrealistic because the Balmer lines are too strong on the metal-poor side to be compatible with observations.

Cold Spring Harbor symposia on quantitative biology: Volume 49, Recombination at the DNA level

International Nuclear Information System (INIS)

1984-01-01

This volume contains full papers prepared by the participants to the 1984 Cold Springs Harbor Symposia on Quantitative Biology. This year's theme is entitled Recombination at the DNA level. The volume consists of 93 articles grouped into subject areas entitled chromosome mechanics, yeast systems, mammalian homologous recombination, transposons, mu, plant transposons/T4 recombination, topoisomerase, resolvase and gyrase, Escherichia coli general recombination, RecA, repair, leukaryotic enzymes, integration and excision of bacteriophage, site-specific recombination, and recombination in vitro

Enhancing photocatalysis in SrTiO3 by using Ag nanoparticles: A two-step excitation model for surface plasmon-enhanced photocatalysis

International Nuclear Information System (INIS)

Ma, Lei; Sun, Tao; Cai, Hua; Zhou, Zhi-Quan; Sun, Jian; Lu, Ming

2015-01-01

Surface plasmon (SP)-enhanced ultraviolet and visible photocatalytic activities of SrTiO 3 (STO) are observed after incorporating Ag nanoparticles (Ag-NPs) on STO surfaces. A two-step excitation model is proposed to explain the SP-enhanced photocatalysis. The point of the model is that an electron at the valence band of STO is first excited onto the Fermi level of Ag-NP by the SP field generated on the Ag-NP, and then injected into the conduction band of STO from the SP band, leaving a hole at the valence band of STO. A full redox catalytic reaction at the surface of STO is then available. For Ag-NP incorporated STO, up-converted and inter-band photoluminescence emissions of STO are observed, and nonlinear evolutions of photocatalytic activity with illumination light powers are found. Furthermore, near infrared photocatalysis is detected. These results support the proposed model

Seroprevalence of Antibodies against Seal Influenza A(H10N7) Virus in Harbor Seals and Gray Seals from the Netherlands.

Science.gov (United States)

Bodewes, Rogier; Rubio García, Ana; Brasseur, Sophie M; Sanchez Conteras, Guillermo J; van de Bildt, Marco W G; Koopmans, Marion P G; Osterhaus, Albert D M E; Kuiken, Thijs

2015-01-01

In the spring and summer 2014, an outbreak of seal influenza A(H10N7) virus infection occurred among harbor seals (Phoca vitulina) off the coasts of Sweden and Denmark. This virus subsequently spread to harbor seals off the coasts of Germany and the Netherlands. While thousands of seals were reported dead in Sweden, Denmark and Germany, only a limited number of seals were found dead in the Netherlands. To determine the extent of exposure of seals in the Netherlands to influenza A/H10N7 virus, we measured specific antibody titers in serum samples from live-captured seals and seals admitted for rehabilitation in the Netherlands by use of a hemagglutination inhibition assay and an ELISA. In harbor seals in 2015, antibodies against seal influenza A(H10N7) virus were detected in 41% (32 out of 78) pups, 10% (5 out of 52) weaners, and 58% (7 out of 12) subadults or adults. In gray seals (Halichoerus grypus) in 2015, specific antibodies were not found in the pups (n = 26), but in 26% (5 out of 19) of the older animals. These findings indicate that, despite apparent low mortality, infection with seal influenza A(H10N7) virus was geographically widespread and also occurred in grey seals.

Analysis and Enhancement of IEEE 802.15.4e DSME Beacon Scheduling Model

Directory of Open Access Journals (Sweden)

Kwang-il Hwang

2014-01-01

Full Text Available In order to construct a successful Internet of things (IoT, reliable network construction and maintenance in a sensor domain should be supported. However, IEEE 802.15.4, which is the most representative wireless standard for IoT, still has problems in constructing a large-scale sensor network, such as beacon collision. To overcome some problems in IEEE 802.15.4, the 15.4e task group proposed various different modes of operation. Particularly, the IEEE 802.15.4e deterministic and synchronous multichannel extension (DSME mode presents a novel scheduling model to solve beacon collision problems. However, the DSME model specified in the 15.4e draft does not present a concrete design model but a conceptual abstract model. Therefore, in this paper we introduce a DSME beacon scheduling model and present a concrete design model. Furthermore, validity and performance of DSME are evaluated through experiments. Based on experiment results, we analyze the problems and limitations of DSME, present solutions step by step, and finally propose an enhanced DSME beacon scheduling model. Through additional experiments, we prove the performance superiority of enhanced DSME.

Model Parameter Variability for Enhanced Anaerobic Bioremediation of DNAPL Source Zones

Science.gov (United States)

Mao, X.; Gerhard, J. I.; Barry, D. A.

2005-12-01

The objective of the Source Area Bioremediation (SABRE) project, an international collaboration of twelve companies, two government agencies and three research institutions, is to evaluate the performance of enhanced anaerobic bioremediation for the treatment of chlorinated ethene source areas containing dense, non-aqueous phase liquids (DNAPL). This 4-year, 5.7 million dollars research effort focuses on a pilot-scale demonstration of enhanced bioremediation at a trichloroethene (TCE) DNAPL field site in the United Kingdom, and includes a significant program of laboratory and modelling studies. Prior to field implementation, a large-scale, multi-laboratory microcosm study was performed to determine the optimal system properties to support dehalogenation of TCE in site soil and groundwater. This statistically-based suite of experiments measured the influence of key variables (electron donor, nutrient addition, bioaugmentation, TCE concentration and sulphate concentration) in promoting the reductive dechlorination of TCE to ethene. As well, a comprehensive biogeochemical numerical model was developed for simulating the anaerobic dehalogenation of chlorinated ethenes. An appropriate (reduced) version of this model was combined with a parameter estimation method based on fitting of the experimental results. Each of over 150 individual microcosm calibrations involved matching predicted and observed time-varying concentrations of all chlorinated compounds. This study focuses on an analysis of this suite of fitted model parameter values. This includes determining the statistical correlation between parameters typically employed in standard Michaelis-Menten type rate descriptions (e.g., maximum dechlorination rates, half-saturation constants) and the key experimental variables. The analysis provides insight into the degree to which aqueous phase TCE and cis-DCE inhibit dechlorination of less-chlorinated compounds. Overall, this work provides a database of the numerical