El Proceso de Bolonia y las nuevas competencias / The Bologna Process and the new skills

Directory of Open Access Journals (Sweden)

Marisa Montero Curiel

2010-09-01

Full Text Available Resumen: La construcción del Espacio Europeo de Educación Superior (EEES, más conocido como Proceso de Bolonia, constituye una magnífica oportunidad para que las universidades aborden un conjunto de reformas que les permita adaptarse a la nueva realidad social, la llamada Sociedad del Conocimiento, reformas orientadas en múltiples direcciones: en las metodologías docentes, en la estructura de las enseñanzas, en la garantía de los procesos de aprendizaje o en la calidad y, por supuesto, en potenciar la movilidad de estudiantes y profesores. En definitiva, es una transformación que afecta de lleno al concepto de la educación superior.La competencia se presenta como un fin que el estudiante deberá lograr en su fase universitaria. En esta nueva perspectiva, el papel del estudiante se modifica y cobra un significado especial; primero, porque él mismo deberá ser el motor que genere su aprendizaje y, segundo, porque no sólo aprenderá dentro de las instituciones superiores, sino que cualquier situación y experiencia educativa podrá acercarle al conocimiento a lo largo de toda su vida. También el profesor se ve sometido a una gran reforma: ahora no sólo tendrá que transmitir una serie de contenidos, sino que el enfoque deberá encaminar a abrir al alumno las puertas a un futuro profesional más amplio. Para ello, será fundamental una enseñanza coordinada, con mayor carga práctica y con una diversidad docente a la que quizá el sistema educativo español no siempre ha estado acostumbrado. Abstract: The construction of the European Space for Higer Education (ESHE, commonly known as the Bologna Process, is a great opportunity for universities to address a set of reforms that will enable the adaptation of the new social reality, the so-called Knowledge Society. These reforms are oriented in multiple directions: the teaching methodology, the structure of education, the guarantee of the learning process or quality and, of course, the

Produciendo el éxito en la política pública: la Transferencia del Manejo de Riego en México

NARCIS (Netherlands)

Rap, E.R.

2008-01-01

La política mexicana para la transferencia del manejo de riego se ha propagado ampliamente como exitosa y ha llegado a ser un modelo para otros países que quieren mejorar el desempeño de sus sistemas de riego y reducir el gasto público. Este artículo analiza el proceso de la creación de la política

Relación entre actitudes y acciones de aprendizaje de los estudiantes universitarios || Connection between attitudes towards studies and learning actions among university students

Directory of Open Access Journals (Sweden)

Marina Elias

2014-07-01

Full Text Available En este artículo se presentan los resultados de una investigación que tiene como objetivo analizar los factores que influyen en el aprendizaje de los estudiantes universitarios. En el marco del proceso de Bolonia y los cambios docentes que éste ha comportado se quiere constatar si realmente el paradigma de enseñanza/aprendizaje centrado en el estudiante ha implicado un aumento del aprendizaje profundo. A partir de un cuestionario contestado por 857 estudiantes de 10 titulaciones de 4 universidades públicas de la Región Metropolitana de Barcelona, se analiza la relación entre motivación de elección de estudios, concepciones de aprendizaje y acciones de aprendizaje, y como estas últimas pueden estar condicionadas por el contexto educativo de las asignaturas. Se concluye que las acciones de aprendizaje están influidas tanto por el contexto como por la orientación profesional de los estudios. Así, los estudiantes llevan a cabo acciones más profundas en las asignaturas caracterizadas por la metodología impulsada con Bolonia, aunque es más beneficioso para un tipo de actitudes hacia el estudio que para otras.

Probable maximum flood on the Ha Ha River

International Nuclear Information System (INIS)

Damov, D.; Masse, B.

1997-01-01

Results of a probable maximum flood (PMF) study conducted for various locations along the Ha Ha river, a tributary of the Saguenay River, were discussed. The study was undertaken for use in the design and construction of new hydraulic structures for water supply for a pulp and paper facility, following the Saguenay Flood in July 1996. Many different flood scenarios were considered, including combinations of snow-melt with rainfall. Using computer simulations, it was shown that the largest flood flows were generated by summer-fall PMF. 5 refs., 12 figs

HA-Coated Implant

DEFF Research Database (Denmark)

Daugaard, Henrik; Søballe, Kjeld; Bechtold, Joan E

2014-01-01

of improving the fixation of implants. Of these, hydroxyapatite (HA) is the most widely used and most extensively investigated. HA is highly osseoconductive, and the positive effect is well documented in both basic and long-term clinical research [1–6]. This chapter describes experimental and clinical studies...

Morphological Effects of HA on the Cell Compatibility of Electrospun HA/PLGA Composite Nanofiber Scaffolds

Directory of Open Access Journals (Sweden)

Adnan Haider

2014-01-01

Full Text Available Tissue engineering is faced with an uphill challenge to design a platform with appropriate topography and suitable surface chemistry, which could encourage desired cellular activities and guide bone tissue regeneration. To develop such scaffolds, composite nanofiber scaffolds of nHA and sHA with PLGA were fabricated using electrospinning technique. nHA was synthesized using precipitation method, whereas sHA was purchased. The nHA and sHA were suspended in PLGA solution separately and electrospun at optimized electrospinning parameters. The composite nanofiber scaffolds were characterized by FE-SEM, EDX analysis, TEM, XRD analysis, FTIR, and X-ray photoelectron. The potential of the HA/PLGA composite nanofiber as bone scaffolds in terms of their bioactivity and biocompatibility was assessed by culturing the osteoblastic cells onto the composite nanofiber scaffolds. The results from in vitro studies revealed that the nHA/PLGA composite nanofiber scaffolds showed higher cellular adhesion, proliferation, and enhanced osteogenesis performance, along with increased Ca+2 ions release compared to the sHA/PLGA composite nanofiber scaffolds and pristine PLGA nanofiber scaffold. The results show that the structural dependent property of HA might affect its potential as bone scaffold and implantable materials in regenerative medicine and clinical tissue engineering.

Design and fabrication of carbon fibers with needle-like nano-HA coating to reinforce granular nano-HA composites.

Science.gov (United States)

Wang, Xudong; Zhao, Xueni; Zhang, Li; Wang, Wanying; Zhang, Jing; He, Fuzhen; Yang, Jianjun

2017-08-01

Carbon fibers (CFs) with needle-like nano-hydroxyapatite (nHA) coating were first used as reinforcing materials named nHA-CFs to improve the mechanical properties of pure HA. A powder mixture containing nHA-CFs and granular nano-HA (gHA) was directly sintered by hot pressing at appropriate sintering pressure and temperature. A three-phase nHA-CFs/gHA composite was designed, fabricated, and used as an artificial bone. Results show that the bending strengths of the nHA-CFs/gHA composite are approximately 41.1% and 59.2% higher than those of CFs/gHA composite and pure HA, respectively. The possible reinforcing mechanism of nHA-CFs in the composite is also proposed at the end. When nHA-CFs are applied for preparation of nHA-CFs/gHA composites, the internal stress on its phase boundary with gHA matrix generated during cooling of sintered is significantly reduced due to the presence of the nHA coatings. It infers that nHA coatings on CFs might act as a bridge to control the forming of interfacial gaps between the gHA matrix and the CFs effectively. Our work provides additional insights into the feasibility of nHA-CFs/gHA composites as load-bearing implant materials in clinical applications. Copyright © 2017 Elsevier B.V. All rights reserved.

Reconocimiento de gestos en tiempo real basado en heurísticas y reconocimiento de patrones

OpenAIRE

López Rincón, Omar

2015-01-01

Desde los inicios de la computación, el área de procesado de imagen se ha ido incrementando y llegando a nuevas fronteras gracias al incremento del poder computacional que no se detiene, más rutinas han llegado a ser posibles, entre ellas el procesado de imagen en tiempo real. El desarrollo en el procesado de imagen ha ido incrementando junto con el desarrollo de nuevos procesadores y velocidad de computo, esto mismo ha ido abarcando desde imágenes satelitales, medicina y hoy en día los prime...

El Gobierno del mal menor

DEFF Research Database (Denmark)

Agustin, Oscar Garcia

2012-01-01

El Gobierno no aspira a ser bueno ni malo. Se conforma con ser el Gobierno del mal menor, cuyas medidas drásticas y poco populares están destinadas a salvar a los ciudadanos del desastre que no ha llegado todavía pero podría llegar....

Modelado de transductores ultrasónicos piezoeléctricos para fisioterapia

Directory of Open Access Journals (Sweden)

Elvira Iglesias

2015-11-01

En este trabajo, usando herramientas de elementos finitos y de análisis vibracional por interferometría láser, se ha llegado a una solución de vibración tipo pistón que permite aplicar correctamente las dosis de presión acústica.

Effect of n-HA with different surface-modified on the properties of n-HA/PLGA composite

Energy Technology Data Exchange (ETDEWEB)

Jiang Liuyun, E-mail: jlytxg@163.com [Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041 (China); Xiong Chengdong; Chen Dongliang [Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041 (China); Jiang Lixin [Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041 (China); Graduated School of Chinese Academy of Sciences, Beijing 100039 (China); Pang Xiubing [Zhejiang Apeloa Medical Technology Co. Ltd, Jinhua 322118 (China)

2012-10-15

Graphical abstract: The bend strength of n-HA/PLGA composite with the unmodified n-HA becomes lower than that of PLGA. However, when n-HA was modified by different methods, the bend strength of g-n-HA/PLGA composites gets a little increase than PLGA, and the g3-n-HA/PLGA shows the highest bend strength at 3% g3-n-HA loading amount in weight, reached 162 MPa, which was 24.4% higher than that of pure PLGA. Highlights: Black-Right-Pointing-Pointer A new surface modification method for n-HA of combining stearic acid with surface-grafting L-lactic was adopted. Black-Right-Pointing-Pointer Three different surface modification methods for n-HA were compared in detail. Black-Right-Pointing-Pointer The new surface modification method was the most ideal method in this study. Black-Right-Pointing-Pointer The g3-n-HA/PLGA composite had the highest bending strength, which would be potential to be used as bone fracture internal fixation materials. - Abstract: Three different surface modification methods for nano-hydroxyapatite (n-HA) of stearic acid, grafted with L-lactide, combining stearic acid and surface-grafting L-lactic were adopted, respectively. The surface modification reaction and the effect of different methods were evaluated by Fourier transformation infrared (FTIR), X-ray photoelectron spectra (XPS), thermal gravimetric analysis (TGA), transmission electron microscopy (TEM). The results showed that n-HA surfaces were all successful modified, and the modification method of combining stearic acid and surface-grafting L-lactic had the greatest grafting amount and the best dispersion among the three modification methods. Then, the n-HA with three different surface modification and unmodified n-HA were introduced into PLGA, respectively, and a serials of n-HA/PLGA composites with 3% n-HA amount in weight were prepared by solution mixing, and the properties of n-HA/PLGA composites were also investigated by electromechanical universal tester and scanning electron

El Dibujo como Herramienta Integral al Servicio del conocimiento en diversos Marcos Disciplinares: Su vigencia y necesidad hoy.

OpenAIRE

García Góngora, Víctor Manuel

2017-01-01

Esta Tesis Doctoral pretende dilucidar mediante obras y experiencias del autor, bibliografía y referencias externas, la vigencia del dibujo en una actualidad dominada por métodos digitales que se ha vuelto escéptica sobre su “utilidad” real y que desgraciadamente se está viendo marginada en planes de estudio y desarrollo profesional. La situación ha llegado a tal grado que el dibujo, incluso en disciplinas como las bellas artes y la arquitectur...

Análisis comparativo de la asignatura "organización del centro escolar" en el nuevo y antiguo plan de estudios de las universidades públicas de Madrid

Directory of Open Access Journals (Sweden)

Alexandra Tardón Morlanes

2012-10-01

Full Text Available En la actualidad los estudios universitarios se encuentran en un proceso de cambio. España ha apostado desde el principio por ese gran proyecto de ciudadanía europea que constituye el Proceso de Bolonia. Ha puesto todas las bases institucionales y jurídicas necesarias para implantar un sistema universitario capaz de formar personas con espíritu cívico, fundamentos intelectuales y capacidad profesional reconocidos en toda Europa, y más allá. No obstante, no son pocas las voces que se han levantado en contra de este nuevo plan de estudios. La realidad es que existe, actualmente, un pacto firmado por los ministros de educación Europeos que ha llegado casi sin previo aviso y que ya se encuentra implantado en todas nuestras Universidades ante el desconcierto general de los estudiantes. Son muchos los que se han movilizado, reclamando en términos generales, la no privatización de la enseñanza. Dado que este es un proceso de cambio en la universidad y dado que los estudiantes, como parte activa de la comunidad universitaria, tenemos la responsabilidad de velar por la mejora de los nuevos planes, nos planteamos la necesidad de analizar la forma en que los estudios de Grado afectan o modifican las asignaturas que en ellos se imparten. Nos centraremos, concretamente, en el análisis de la asignatura de “Organización del centro escolar” en las diferentes universidades españolas. En este sentido y como estudiantes de Magisterio de Ed. Primaria (especialidad en lengua extranjera, nos proponemos, en este trabajo de investigación, analizar los cambios pertinentes que se han producido en la asignatura de “Organización del centro escolar” y realizar una comparación de la asignatura respecto al plan antiguo y al nuevo. Desde esta perspectiva, nos planteamos como objetivo principal estudiar los cambios concretos que pueden observarse en dicha asignatura y valorar cómo éstos pueden beneficiar o perjudicar a los estudiantes universitarios

El proceso de profesionalización del periodista en España o la historia de nunca acabar

OpenAIRE

Lozano, Jesús Díaz del Campo

2012-01-01

El periodismo ha recorrido un largo camino para llegar a ser una profesión. En esteartículo se analiza la situación de los periodistas en España, para tratar de averiguarsi la actividad periodística ha alcanzado ese estatus.Existe un alto grado de consenso entre los expertos sobre cuáles son las característicasprincipales para poder decir que una ocupación ha llegado a ser una profesión: setrata de una ocupación a tiempo completo, cuenta con un código ético, se necesitaformación oficial para ...

Hiperuricemia y gota: el papel de la dieta

OpenAIRE

Bonifacio Álvarez-Lario; J. L. Alonso-Valdivielso

2014-01-01

Desde la antigüedad se ha relacionado a la gota con los excesos en el comer y en el beber; sin embargo, ha sido en la última década cuando se ha llegado a un mayor conocimiento sobre los factores dietéticos asociados con el desarrollo de hiperuricemia y gota. La obesidad, el abuso en la ingesta de carnes y las bebidas alcohólicas ya eran vistos como culpables desde la antigüedad. Las legumbres y las verduras ricas en purinas han sido exculpadas tras los estudios. Se han descrito nuevos factor...

Antivídeo, resistencia al imperativo publicitario. Definición y clasificación

Directory of Open Access Journals (Sweden)

Ricardo RONCERO-PALOMAR

2017-07-01

Full Text Available El antivídeo es un tipo de vídeo musical que huye del lenguaje visual heredero de la publicidad. Se trata de un producto de difícil clasificación que se encuentra a mitad de camino entre la producción artística, el marketing y la resistencia anti comercial. La falta de una definición que ancle su significado y la necesidad de un análisis que permita conocer sus orígenes y motivaciones lo ha convertido en una de las variables del videoclip menos estudiado hasta la fecha. El término nunca ha llegado a estar muy extendido, quizá porque estas piezas, al ser poco emitidas por televisión no han llegado a ser muy populares. Esta investigación busca definir el término y estudiar cuáles han sido las motivaciones que han llevado a la aparición de esta modalidad de vídeo musical y conocer así sus posibles variables.

El aprendizaje en la era de la modulación: consecuencias del Proceso de Bolonia para la Enseñanza Superior

Directory of Open Access Journals (Sweden)

Ewald Terhart

2006-01-01

Full Text Available El autor discute a partir de su propia experiencia en diversas universidades los presupuestos, formas y repercusiones de la reestructuración de la enseñanza superior en dirección a una «modularización». Esta forma de configuración de los estudios constituye un elemento esencial en el así llamado «Proceso de Bolonia». Tras una exposición de los orígenes del concepto de modularización, el autor analiza las consecuencias y los problemas concretos derivados de la introducción de la modularización en facultades e institutos universitarios. Por lo que atañe a las universidades alemanas, esta modularización representa para muchas asignaturas un grado muy elevado de escolarización de los estudios. La reglamentación a que deben someterse tanto estudiantes como docentes se hace mucho mayor. Al mismo tiempo, también aumenta el potencial conflictivo en el seno del profesorado. Dado que la modularización provoca un crecimiento muy notable tanto de la demanda de clases, como de las tareas burocráticas que conllevan los exámenes y la extensión de certificados, pero ello sin que pueda esperarse un aumento paralelo de personal docente, la modularización incide notablemente en la configuración del puesto de trabajo del profesor universitario 

Adhesive strength of hydroxyl apatite(HA coating and biomechanics behavior of HA-coated prosthesis:an experimental study

Directory of Open Access Journals (Sweden)

Tian-yang ZHANG

2011-05-01

Full Text Available Objective To explore the influence of adhesive strength of hydroxyapatite(HA coating on the post-implantation stability of HA-coated prosthesis.Methods The adhesive strength and biomechanics behavior of HA coating were studied by histopathological observation,material parameters and biomechanical testing,the titanium(Ti-coated prosthesis was employed as control.Results Scratch test showed that the adhesive strength of HA coating was significantly lower than that of Ti coating(P < 0.01.Histopathological examination and bone morphometry showed that,at the early stage of prosthesis implantation,the bony growth around HA-coated prosthesis was significantly higher than that around Ti-coated prosthesis(P < 0.01,but the ultimate shear strength of HA-coated prosthesis was much lower than that of Ti-coated prosthesis(P < 0.01.After the push-out test with prosthesis,histopathological observation showed that there were accumulations of clump-and strip-like granular residues on the surface of bones that newly grew around the HA-coated prosthesis,and surface energy-dispersive X-ray spectroscopy(EDX analysis also confirmed that the shear stress induced HA decohesion from the substrate of prosthesis.Conclusions Although HA coating showed a satisfactory effect on early bone formation and prosthetic stability,due to the deficiencies of adhesive strength,the early stability of prosthesis may be gradually destroyed by the shear loads of human body and coating degradation.

Torres

Directory of Open Access Journals (Sweden)

Horacio Torres

1987-05-01

Full Text Available Ingeniero Eléctrico y catedrático de la Universidad Nacional. Recién llegado al autodidactismo del dibujo. La amnistía decretada por los humoristas gráficos le ha permitido salir de la clandestinidad y hacer público su trabajo. Mejor humorista que dibujante y por sobre todo un entusiasta del humor.

Adhesive strength of hydroxyl apatite(HA) coating and biomechanics behavior of HA-coated prosthesis:an experimental study

OpenAIRE

Tian-yang ZHANG; Yong-hong DUAN; Shu ZHU; Jin-yu ZHU; Qing-sheng ZHU

2011-01-01

Objective To explore the influence of adhesive strength of hydroxyapatite(HA) coating on the post-implantation stability of HA-coated prosthesis.Methods The adhesive strength and biomechanics behavior of HA coating were studied by histopathological observation,material parameters and biomechanical testing,the titanium(Ti)-coated prosthesis was employed as control.Results Scratch test showed that the adhesive strength of HA coating was significantly lower than that of Ti coating(P < 0.01).Hist...

The HaDREB2 transcription factor enhances basal thermotolerance and longevity of seeds through functional interaction with HaHSFA9

Directory of Open Access Journals (Sweden)

Carranco Raúl

2009-06-01

Full Text Available Abstract Background Transcription factor HaDREB2 was identified in sunflower (Helianthus annuus L. as a drought-responsive element-binding factor 2 (DREB2 with unique properties. HaDREB2 and the sunflower Heat Shock Factor A9 (HaHSFA9 co-activated the Hahsp17.6G1 promoter in sunflower embryos. Both factors could be involved in transcriptional co-activation of additional small heat stress protein (sHSP promoters, and thus contribute to the HaHSFA9-mediated enhancement of longevity and basal thermotolerance of seeds. Results We found that overexpression of HaDREB2 in seeds did not enhance longevity. This was deduced from assays of basal thermotolerance and controlled seed-deterioration, which were performed with transgenic tobacco. Furthermore, the constitutive overexpression of HaDREB2 did not increase thermotolerance in seedlings or result in the accumulation of HSPs at normal growth temperatures. In contrast, when HaDREB2 and HaHSFA9 were conjointly overexpressed in seeds, we observed positive effects on seed longevity, beyond those observed with overexpression of HaHSFA9 alone. Such additional effects are accompanied by a subtle enhancement of the accumulation of subsets of sHSPs belonging to the CI and CII cytosolic classes. Conclusion Our results reveal the functional interdependency of HaDREB2 and HaHSFA9 in seeds. HaDREB2 differs from other previously characterized DREB2 factors in plants in terms of its unique functional interaction with the seed-specific HaHSFA9 factor. No functional interaction between HaDREB2 and HaHSFA9 was observed when both factors were conjointly overexpressed in vegetative tissues. We therefore suggest that additional, seed-specific factors, or protein modifications, could be required for the functional interaction between HaDREB2 and HaHSFA9.

La profilaxis de las infecciones puerperales por los derivados de la sulfanilamida

Directory of Open Access Journals (Sweden)

Hernando Acosta S.

1940-04-01

ha sido siempre un tema de actualidad. La obstetricia moderna ha llegado a resolver multitud de problemas terapéuticos que constituían un verdadero rompecabezas para los parteros de épocas relativamente recientes. Con todo, hoy persisten, sin solución satisfactoria, problemas de tan vital importancia como el de las gestosis y el de las formas graves de infección puerperal, contra las cuales vemos fracasar día por día, la mayor parte de las medidas tera péuticas aconsejadas. Quizá esto mismo ha servido de base para orientar las modernas investigaciones por un rumbo más lógico.

La importancia del eBranding en el posicionamiento de la empresa digital. Análisis de un caso de éxito: la marca Mr. Wonderful

OpenAIRE

González Reboredo, Alba

2016-01-01

La gestión estratégica de la marca se ha vuelto uno de los activos más importantes a la hora de generar beneficios más allá de lo tangible. Este elemento, también conocido como branding, ha evolucionado hasta inmiscuirse en el entorno online, lo que hoy en día se denomina como eBranding. En este trabajo se mostrará cómo ha llegado el eBranding a constituirse como uno de los elementos más relevantes de una marca. Para ello se partirá de un estudio teórico en el que se tratará el branding de...

El ascenso de la soja en Paraguay : Un estudio de la demanda mundial por la soja

OpenAIRE

Almerfors, Jacqueline

2008-01-01

La producción de soja ha crecido significativamente durante un periodo de 6 años en Paraguay llegado a una cosecha record de 6,2 millones de toneladas en 2007. Este aumento se debe a factores globales como la demanda creciente por la soja para la Unión Europea que va destinada a la producción del biocombustible. El objetivo de este estudio ha sido investigar cual fue la causa que conllevo al aumento de la producción de soja en Paraguay. Con la ayuda de principalmente dos publicaciones, Republ...

La responsabilidad de proteger. Una nueva dimensión de la soberanía

OpenAIRE

Vallejo Franco, Beatriz Eugenia

2010-01-01

El concepto de soberanía ha sido objeto de grandes controversias, especialmente desde el siglo xx. De la noción de un Estado-nación rodeado de fronteras infranqueables, derivada del Tratado de Westfalia, se ha llegado a la idea de entes estatales cuyos asuntos son permeados constantemente por la actuación de la comunidad internacional. La pregunta que se intenta despejar aquí es si la internacionalización de los derechos humanos hace parte de los temas que hoy en día interfieren con la sobera...

El lenguaje de la piedra y su pervivencia en la escultura actual

OpenAIRE

Olano Sans, Ana María

2016-01-01

La piedra ha sobrevivido al paso del tiempo, aparece en todas las manifestaciones artísticas y culturales como un material irreemplazable en la representación tridimensional. Fue el primer aliado que tuvo el ser humano en su confrontación con el medio natural, dejando una profunda huella en nuestra memoria cultural y genética. Artistas de antes y de hoy continúan aprovechando sus propiedades físicas y simbólicas, de modo que su valor no ha llegado a disolverse del todo en la multidireccionali...

Molecular aspects of zygotic embryogenesis in sunflower (Helianthus annuus L.): correlation of positive histone marks with HaWUS expression and putative link HaWUS/HaL1L.

Science.gov (United States)

Salvini, Mariangela; Fambrini, Marco; Giorgetti, Lucia; Pugliesi, Claudio

2016-01-01

The link HaWUS/ HaL1L , the opposite transcriptional behavior, and the decrease/increase in positive histone marks bond to both genes suggest an inhibitory effect of WUS on HaL1L in sunflower zygotic embryos. In Arabidopsis, a group of transcription factors implicated in the earliest events of embryogenesis is the WUSCHEL-RELATED HOMEOBOX (WOX) protein family including WUSCHEL (WUS) and other 14 WOX protein, some of which contain a conserved WUS-box domain in addition to the homeodomain. WUS transcripts appear very early in embryogenesis, at the 16-cell embryo stage, but gradually become restricted to the center of the developing shoot apical meristem (SAM) primordium and continues to be expressed in cells of the niche/organizing center of SAM and floral meristems to maintain stem cell population. Moreover, WUS has decisive roles in the embryonic program presumably promoting the vegetative-to-embryonic transition and/or maintaining the identity of the embryonic stem cells. However, data on the direct interaction between WUS and key genes for seed development (as LEC1 and L1L) are not collected. The novelty of this report consists in the characterization of Helianthus annuus WUS (HaWUS) gene and in its analysis regarding the pattern of the methylated lysine 4 (K4) of the Histone H3 and of the acetylated histone H3 during the zygotic embryo development. Also, a parallel investigation was performed for HaL1L gene since two copies of the WUS-binding site (WUSATA), previously identified on HaL1L nucleotide sequence, were able to be bound by the HaWUS recombinant protein suggesting a not described effect of HaWUS on HaL1L transcription.

Migración negra en Santa Marta

Directory of Open Access Journals (Sweden)

Josè Luis Vega de Lavalle

2013-11-01

Full Text Available El objetivo de este artículo es dar cuenta de los procesos e inserción urbana de la Gente Negra  se ha instalado en Santa Marta, los cuales han llegado a partir de la década de los años ochenta a  en el sector turístico de esta ciudad.

La BBC de Londres y su realidad actual

Directory of Open Access Journals (Sweden)

Alberto Souviron

2015-01-01

Full Text Available La BBC de Londres es uno de de los principales servicios públicos de radio, televisión e Internet en el mundo. La audiencia de la radio ha llegado a niveles récord: 153 millones de oyentes. La BBC debe competir fuertemente con el nombre y prestigio de la CNN más allá del producto.

Biomedical potential of chitosan/HA and chitosan/β-1,3-glucan/HA biomaterials as scaffolds for bone regeneration — A comparative study

Energy Technology Data Exchange (ETDEWEB)

Przekora, Agata, E-mail: agata.przekora@umlub.pl [Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin (Poland); Palka, Krzysztof [Department of Materials Engineering, Lublin University of Technology, Nadbystrzycka 36, 20-618 Lublin (Poland); Ginalska, Grazyna [Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin (Poland)

2016-01-01

The aim of this work was to compare biomedical potential of chitosan/hydroxyapatite (chit/HA) and novel chitosan/β-1,3-glucan/hydroxyapatite (chit/glu/HA) materials as scaffolds for bone regeneration via characterization of their biocompatibility, porosity, mechanical properties, and water uptake behaviour. Biocompatibility of the scaffolds was assessed in direct-contact with the materials using normal human foetal osteoblast cell line. Cytotoxicity and osteoblast proliferation rate were evaluated. Porosity was assessed using computed microtomography analysis and mechanical properties were determined by compression testing. Obtained results demonstrated that chit/HA scaffold possessed significantly better mechanical properties (compressive strength: 1.23 MPa, Young's modulus: 0.46 MPa) than chit/glu/HA material (compressive strength: 0.26 MPa, Young's modulus: 0.25 MPa). However, addition of bacterial β-1,3-glucan to the chit/HA scaffold improved its flexibility and porosity. Moreover, chit/glu/HA scaffold revealed significantly higher water uptake capability (52.6% after 24 h of soaking) compared to the chit/HA (30.7%) and thus can serve as a very good drug delivery carrier. Chit/glu/HA scaffold was also more favourable to osteoblast survival (near 100% viability after 24-h culture), proliferation, and spreading compared to the chit/HA (63% viability). The chit/glu/HA possesses better biomedical potential than chit/HA scaffold. Nevertheless, poor mechanical properties of the chit/glu/HA limit its application to non-load bearing implantation area. - Highlights: • Chitosan/HA and chit/β-1,3-glucan/HA scaffolds for bone regeneration were compared. • Chit/HA significantly reduced osteoblast viability to 63% compared to chit/glu/HA. • Unlike chit/HA, chit/glu/HA favoured cell adhesion, spreading, and proliferation. • Chit/HA had better compressive strength and Young's modulus than chit/glu/HA. • Chit/glu/HA revealed significantly higher

Osteoblast interaction with laser cladded HA and SiO2-HA coatings on Ti-6Al-4V

International Nuclear Information System (INIS)

Yang Yuling; Serpersu, Kaan; He Wei; Paital, Sameer R.; Dahotre, Narendra B.

2011-01-01

In order to improve the bioactivity and biocompatibility of titanium endosseous implants, the morphology and composition of the surfaces were modified. Polished Ti-6Al-4V substrates were coated by a laser cladding process with different precursors: 100 wt.% HA and 25 wt.% SiO 2 -HA. X-ray diffraction of the laser processed samples showed the presence of CaTiO 3 , Ca 3 (PO 4 ) 2 , and Ca 2 SiO 4 phases within the coatings. From in vitro studies, it was observed that compared to the unmodified substrate all laser cladded samples presented improved cellular interactions and bioactivity. The samples processed with 25 wt.% SiO 2 -HA precursor showed a significantly higher HA precipitation after immersion in simulated body fluid than 100 wt.% HA precursor and titanium substrates. The in vitro biocompatibility of the laser cladded coatings and titanium substrate was investigated by culturing of mouse MC3T3-E1 pre-osteoblast cell line and analyzing the cell viability, cell proliferation, and cell morphology. A significantly higher cell attachment and proliferation rate were observed for both laser cladded 100 wt.% HA and 25 wt.% SiO 2 -HA samples. Compared to 100 wt.% HA sample, 25 wt.% SiO 2 -HA samples presented a slightly improved cellular interaction due to the addition of SiO 2 . The staining of the actin filaments showed that the laser cladded samples induced a normal cytoskeleton and well-developed focal adhesion contacts. Scanning electron microscopic image of the cell cultured samples revealed better cell attachment and spreading for 25 wt.% SiO 2 -HA and 100 wt.% HA coatings than titanium substrate. These results suggest that the laser cladding process improves the bioactivity and biocompatibility of titanium. The observed biological improvements are mainly due to the coating induced changes in surface chemistry and surface morphology. Highlights: → Laser cladding of Ti alloys with bioceramics creates new phases. → Laser cladded samples with SiO 2 -doped

Characterization of New PEEK/HA Composites with 3D HA Network Fabricated by Extrusion Freeforming.

Science.gov (United States)

Vaezi, Mohammad; Black, Cameron; Gibbs, David M R; Oreffo, Richard O C; Brady, Mark; Moshrefi-Torbati, Mohamed; Yang, Shoufeng

2016-05-26

Addition of bioactive materials such as calcium phosphates or Bioglass, and incorporation of porosity into polyetheretherketone (PEEK) has been identified as an effective approach to improve bone-implant interfaces and osseointegration of PEEK-based devices. In this paper, a novel production technique based on the extrusion freeforming method is proposed that yields a bioactive PEEK/hydroxyapatite (PEEK/HA) composite with a unique configuration in which the bioactive phase (i.e., HA) distribution is computer-controlled within a PEEK matrix. The 100% interconnectivity of the HA network in the biocomposite confers an advantage over alternative forms of other microstructural configurations. Moreover, the technique can be employed to produce porous PEEK structures with controlled pore size and distribution, facilitating greater cellular infiltration and biological integration of PEEK composites within patient tissue. The results of unconfined, uniaxial compressive tests on these new PEEK/HA biocomposites with 40% HA under both static and cyclic mode were promising, showing the composites possess yield and compressive strength within the range of human cortical bone suitable for load bearing applications. In addition, preliminary evidence supporting initial biological safety of the new technique developed is demonstrated in this paper. Sufficient cell attachment, sustained viability in contact with the sample over a seven-day period, evidence of cell bridging and matrix deposition all confirmed excellent biocompatibility.

Characterization of New PEEK/HA Composites with 3D HA Network Fabricated by Extrusion Freeforming

Directory of Open Access Journals (Sweden)

Mohammad Vaezi

2016-05-01

Full Text Available Addition of bioactive materials such as calcium phosphates or Bioglass, and incorporation of porosity into polyetheretherketone (PEEK has been identified as an effective approach to improve bone-implant interfaces and osseointegration of PEEK-based devices. In this paper, a novel production technique based on the extrusion freeforming method is proposed that yields a bioactive PEEK/hydroxyapatite (PEEK/HA composite with a unique configuration in which the bioactive phase (i.e., HA distribution is computer-controlled within a PEEK matrix. The 100% interconnectivity of the HA network in the biocomposite confers an advantage over alternative forms of other microstructural configurations. Moreover, the technique can be employed to produce porous PEEK structures with controlled pore size and distribution, facilitating greater cellular infiltration and biological integration of PEEK composites within patient tissue. The results of unconfined, uniaxial compressive tests on these new PEEK/HA biocomposites with 40% HA under both static and cyclic mode were promising, showing the composites possess yield and compressive strength within the range of human cortical bone suitable for load bearing applications. In addition, preliminary evidence supporting initial biological safety of the new technique developed is demonstrated in this paper. Sufficient cell attachment, sustained viability in contact with the sample over a seven-day period, evidence of cell bridging and matrix deposition all confirmed excellent biocompatibility.

Educación para el Ocio: Ha llegado la hora de un nuevo objetivo para una vieja idea.

Directory of Open Access Journals (Sweden)

Susan Hutchinson

2012-01-01

Full Text Available La Educación para el Ocio nos sitúa ante una vieja idea, que está siendo examinada con renovado vigor en Canadá. Profesionales e investigadores de diversas disciplinas (por ejemplo, en el ámbito de Salud, la Justicia, la Educación o la Recreación reconocen, cada vez más, que son muchas las personas que pueden beneficiarse del ocio y de las oportunidades de recreación en sus comunidades, aún cuando son bastantes las no pueden hacerlo porque carecen de alfabetización en ocio. Esto es, de los conocimientos, habilidades y confianza que se necesitan para darle un significado personal, que incida positivamente en su bienestar y calidad de vida. La Educación para el Ocio es un medio clave para mejorar la alfabetización en ocio. El texto presenta un modelo de educación para el ocio desarrollado por la Dra. Brenda Robertson, argumentando cómo y por qué la Educación para el Ocio es necesaria para abordar la salud mental y el bienestar de las personas que sufren marginación en sus comunidades. Complementariamente se presentan algunas recomendaciones para avanzar esta Educación, como un componente fundamental de la agenda nacional para la recreación en Canadá.--------------------------------------------------------------------Leisure education is an old idea that is being examined with renewed vigour in Canada. Practitioners and researchers from various disciplines (e.g., health, justice, education, recreation are recognising anew that many people who could benefit from leisure or recreation opportunities in their communities are unable to do so because they lack leisure literacy. Leisure literacy refers to the knowledge, skills and confidence to engage in personally meaningful, health-enhancing leisure. Leisure education is a key means to enhance leisure literacy. The paper presents a model of leisure education developed by Dr. Brenda Robertson and argues for how and why leisure education is needed to address the mental health and well-being needs of persons who experience marginalisation in their communities. Recommendations for moving forward with leisure education as part of a national agenda for recreation in Canada are presented.

Characterization, Corrosion Resistance, and Cell Response of High-Velocity Flame-Sprayed HA and HA/TiO2 Coatings on 316L SS

Science.gov (United States)

Singh, Tejinder Pal; Singh, Harpreet; Singh, Hazoor

2012-09-01

The main aim of this study is to evaluate corrosion and biocompatibility behavior of thermal spray hydroxyapatite (HA) and hydroxyapatite/titania bond (HA/TiO2)-coated 316L stainless steel (316L SS). In HA/TiO2 coatings, TiO2 was used as a bond coat between HA top coat and 316L SS substrate. The coatings were characterized by x-ray diffraction and scanning electron microscopy/energy dispersive spectroscopy, and corrosion resistance determined for the uncoated substrate and the two coatings. The biological behavior was investigated by the cell culture studies using osteosarcoma cell line KHOS-NP (R-970-5). The corrosion resistance of the steel was found to increase after the deposition of the HA and HA/TiO2 bond coatings. Both HA, as well as, HA/TiO2 coatings exhibit excellent bond strength of 49 and 47 MPa, respectively. The cell culture studies showed that HA-coated 316L SS specimens appeared more biocompatible than the uncoated and HA/TiO2-coated 316L SS specimens.

Osteoblast interaction with laser cladded HA and SiO{sub 2}-HA coatings on Ti-6Al-4V

Energy Technology Data Exchange (ETDEWEB)

Yang Yuling [Department of Physics, Northeastern University, Shenyang 110004 (China); Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Serpersu, Kaan [Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); He Wei, E-mail: whe5@utk.edu [Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Department of Mechanical, Aerospace and Biomedical Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Paital, Sameer R. [Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996 (United States); Dahotre, Narendra B. [Department of Materials Science and Engineering, University of North Texas, Denton, TX 76207 (United States)

2011-12-01

In order to improve the bioactivity and biocompatibility of titanium endosseous implants, the morphology and composition of the surfaces were modified. Polished Ti-6Al-4V substrates were coated by a laser cladding process with different precursors: 100 wt.% HA and 25 wt.% SiO{sub 2}-HA. X-ray diffraction of the laser processed samples showed the presence of CaTiO{sub 3}, Ca{sub 3}(PO{sub 4}){sub 2}, and Ca{sub 2}SiO{sub 4} phases within the coatings. From in vitro studies, it was observed that compared to the unmodified substrate all laser cladded samples presented improved cellular interactions and bioactivity. The samples processed with 25 wt.% SiO{sub 2}-HA precursor showed a significantly higher HA precipitation after immersion in simulated body fluid than 100 wt.% HA precursor and titanium substrates. The in vitro biocompatibility of the laser cladded coatings and titanium substrate was investigated by culturing of mouse MC3T3-E1 pre-osteoblast cell line and analyzing the cell viability, cell proliferation, and cell morphology. A significantly higher cell attachment and proliferation rate were observed for both laser cladded 100 wt.% HA and 25 wt.% SiO{sub 2}-HA samples. Compared to 100 wt.% HA sample, 25 wt.% SiO{sub 2}-HA samples presented a slightly improved cellular interaction due to the addition of SiO{sub 2}. The staining of the actin filaments showed that the laser cladded samples induced a normal cytoskeleton and well-developed focal adhesion contacts. Scanning electron microscopic image of the cell cultured samples revealed better cell attachment and spreading for 25 wt.% SiO{sub 2}-HA and 100 wt.% HA coatings than titanium substrate. These results suggest that the laser cladding process improves the bioactivity and biocompatibility of titanium. The observed biological improvements are mainly due to the coating induced changes in surface chemistry and surface morphology. Highlights: {yields} Laser cladding of Ti alloys with bioceramics creates new

EL ABORTO TERAPÉUTICO EN NICARAGUA: EL DIÁLOGO COMO PARTE DE LA SOLUCIÓN AL CONFLICTO

OpenAIRE

Barrantes Monge,Melba de la Cruz; Mercado Morales,Elizabeth

2008-01-01

La derogación de la Ley del Aborto Terapéutico en Nicaragua ha generado un conflicto de opinión en la sociedad nicaragüense y, hasta el momento, no se ha llegado a un consenso en los distintos ámbitos de la sociedad. La Ley es clara en cuanto a prohibir esa práctica, pero es preciso hacer un adecuado análisis del tema, usando el diálogo como herramienta que contemple los principios bioéticos, para entender las implicancias positivas y negativas de la derogación de esta Ley en el binomio madre...

Un análisis de las promociones de ventas al consumidor : los cambios que supone la introducción de los smartphones en esta táctica de marketing

OpenAIRE

Roman Coy, David

2016-01-01

Los dispositivos móviles están cambiando la forma en la que las personas nos relacionamos, nuestros comportamientos, el proceso de búsqueda de información, la manera de pasar el tiempo de ocio y cómo aprendemos. Solomon, Bamossy, Askegaard & Hogg (2013) lo definen como la mobile revolution. La utilización de los dispositivos móviles dentro de nuestra vida diaria ha crecido exponencialmente en los últimos 20 años. Esta evolución ha llegado a su culminación con la introducción, desde 2007, d...

La formación en materias de inteligencia en España. Estado del arte a finales de 2015

Directory of Open Access Journals (Sweden)

Gustavo Díaz Matey

2016-05-01

Full Text Available Todo lo relacionado con la inteligencia ha ido incorporándose progresivamente al debate público de las sociedades democráticas. España no es un caso aparte. En los últimos años, la oferta formativa relacionada con los temas de inteligencia, desde las más variadas perspectivas y desde distintas disciplinas, ha aumentado de forma exponencial. En este estudio hemos analizado la oferta formativa en España del 2012 al 2015 y llegado a diferentes conclusiones que representan el estado de los estudios de inteligencia en la actualidad y el camino a recorrer.

Effect of Water-Glass Coating on HA and HA-TCP Samples for MSCs Adhesion, Proliferation, and Differentiation

Directory of Open Access Journals (Sweden)

Indu Bajpai

2016-01-01

Full Text Available Ca-P and silicon based materials have become very popular as bone tissue engineering materials. In this study, water-glass (also known as sodium silicate glass was coated on sintered hydroxyapatite (HA and HA-TCP (TCP stands for tricalcium phosphate samples and subsequently heat-treated at 600°C for 2 hrs. X-rays diffraction showed the presence of β- and α-TCP phases along with HA in the HA-TCP samples. Samples without coating, with water-glass coating, and heat-treated after water-glass coating were used to observe the adhesion and proliferation response of bone marrow derived-mesenchymal stem cells (MSCs. Cell culture was carried out for 4 hrs, 1 day, and 7 days. Interestingly, all samples showed similar response for cell adhesion and proliferation up to 7-day culture but fibronectin, E-cadherin, and osteogenic differentiation related genes (osteocalcin and osteopontin were significantly induced in heat-treated water-glass coated HA-TCP samples. A water-glass coating on Ca-P samples was not found to influence the cell proliferation response significantly but activated some extracellular matrix genes and induced osteogenic differentiation in the MSCs.

Estudio de caso del Postgrado Comunicación e Imagen del IADE Lisboa

OpenAIRE

Pereira, María Helena Cardoso Simoes

2006-01-01

El Proceso de Bolonia ha creado huevas reglas para la uniformación de la Enseñanza Superior en la Europa, implantando nuevos paradigmas, entre los cuales está el aseguramiento de la calidad y la importancia de la formación a lo largo de la vida. El objetivo de este estudio se inserta en estas dos temáticas, pretendiendo saber si el curso de Postgrado en Comunicac ión e Imagen del IADE (Lisboa) administra una enseñanza de calidad. La metodología utilizada se basó en el modelo EFQM. Se auscultó...

Experiencia piloto de implantación de los ECTS en las enseñanzas de introducción a la Economía en la diplomatura de Turismo de la EUEE

OpenAIRE

Vázquez Bermúdez, Isabel; González Limón, Miryam

2005-01-01

El 25 de mayo de 1998 en La Sorbona (París) se firmó la declaración conjunta para la armonización del diseño del Sistema Europeo de Educación Superior con el objetivo de establecer un área europea abierta a la educación superior. La flexibilidad del sistema se conseguirá mediante el Sistema Europeo de Transferencia de Créditos, que permitirá la convalidación de los créditos en las Universidades europeas. La implantación ha sido recomendada en las sucesivas declaraciones de Bolonia (1999), Pra...

Püha õhtu seltskonnalaud / Kaire Nurk

Index Scriptorium Estoniae

Nurk, Kaire, 1960-

2005-01-01

Püha õhtusöömaaja teemast kunstis. Saksa kunstiajaloolase Horst Schwebeli poolt Leonardo da Vinci "Püha õhtusöömaaja" XX sajandi "töötlustes" välja toodud poliitilise agitatsiooni ja ühisest probleemist haaratud seltskonna kujutiste suunast. Laua kujutisest kunstis

Effect of humic acid (HA) on sulfonamide sorption by biochars

International Nuclear Information System (INIS)

Lian, Fei; Sun, Binbin; Chen, Xi; Zhu, Lingyan; Liu, Zhongqi; Xing, Baoshan

2015-01-01

Effect of quantity and fractionation of loaded humic acid (HA) on biochar sorption for sulfonamides was investigated. The HA was applied in two different modes, i.e. pre-coating and co-introduction with sorbate. In pre-coating mode, the polar fractions of HA tended to interact with low-temperature biochars via H-bonding, while the hydrophobic fractions were likely to be adsorbed by high-temperature biochars through hydrophobic and π-π interactions, leading to different composition and structure of the HA adlayers. The influences of HA fractionation on biochar sorption for sulfonamides varied significantly, depending on the nature of interaction between HA fraction and sorbate. Meanwhile, co-introduction of HA with sulfonamides revealed that the effect of HA on sulfonamide sorption was also dependent on HA concentration. These findings suggest that the amount and fractionation of adsorbed HA are tailored by the surface properties of underlying biochars, which differently affect the sorption for organic contaminants. - Highlights: • Effect of quantity and fractionation of coated HA on sorption of sulfonamides by BC was studied. • Fractionation of coated HA is tailored by surface properties of BC. • Roles of HA in BC sorption depend on interaction between HA adlayer and sorbate. • Roles of HA in sulfonamide sorption by BC also depend on HA aqueous concentration. - The quantity and fractionation of adsorbed HA play a major role in sulfonamide sorption by biochars

Imaging of Homeostatic, Neoplastic, and Injured Tissues by HA-Based Probes

Science.gov (United States)

Veiseh, Mandana; Breadner, Daniel; Ma, Jenny; Akentieva, Natalia; Savani, Rashmin C; Harrison, Rene; Mikilus, David; Collis, Lisa; Gustafson, Stefan; Lee, Ting-Yim; Koropatnick, James; Luyt, Leonard G.; Bissell, Mina J.; Turley, Eva A.

2013-01-01

An increase in hyaluronan (HA) synthesis, cellular uptake, and metabolism occurs during the remodeling of tissue microenvironments following injury and during disease processes such as cancer. We hypothesized that multimodality HA-based probes selectively target and detectably accumulate at sites of high HA metabolism, thus providing a flexible imaging strategy for monitoring disease and repair processes. Kinetic analyses confirmed favorable available serum levels of the probe following intravenous (i.v.) or subcutaneous (s.c.) injection. Nuclear (technetium-HA, 99mTc-HA, and iodine-HA, 125I-HA), optical (fluorescent Texas Red-HA, TR-HA), and magnetic resonance (gadolinium-HA, Gd-HA) probes imaged liver (99mTc-HA), breast cancer cells/xenografts (TR-HA, Gd-HA), and vascular injury (125I-HA, TR-HA). Targeting of HA probes to these sites appeared to result from selective HA receptor-dependent localization. Our results suggest that HA-based probes, which do not require polysaccharide backbone modification to achieve favorable half-life and distribution, can detect elevated HA metabolism in homeostatic, injured, and diseased tissues. PMID:22066590

Las falacias de la ideologia neoliberal

Directory of Open Access Journals (Sweden)

César Tejedor de la Iglesia

2015-02-01

Full Text Available El neoliberalismo se ha convertido en las últimas décadas en la única brújula de la política en el contexto de la globalización económica. Incluso se ha llegado a dar por sentado que el liberalismo económico constituye el “fin de la historia”. La llamada “ciencia” económica trata de dar forma académica a las tesis neoliberales. Este trabajo tiene como objetivo desvelar las falacias que encierra la ideología neoliberal, y mostrar cómo la deriva que ha tomado últimamente la economía política en los países desarrollados no es ni la única posible ni la más beneficiosa posible.

Marketing de ciudades: una necesidad para los procesos de revitalización

OpenAIRE

Elizagarate Gutiérrez, Victoria

1996-01-01

La tendencia del Marketing de las últimas décadas gira sobre el concepto de que cualquier producto o servicio para que tenga éxito, debe de satisfacer las necesidades del grupo de clientes al que se dirige.Es lo que los expertos de Marketing denominan "la orientación al cliente". Este planteamiento, tan propio del mundo empresarial, ha llegado también a aplicarse a ciudades, regiones y países.

Las Matemáticas y sus aplicaciones, ayer y hoy: Retos del futuro

OpenAIRE

Vázquez Suárez, Juan Luis

2013-01-01

Los científicos han sabido desde hace siglos que las matemáticas forman, junto con el método experimental, el esquema conceptual en que se basa la ciencia moderna y en el que se apoya la tecnología. En la presente sociedad de la información, el esquema se ha ampliado y está formado por experimentos, matemáticas y computación. Tras siglos de vivir una vida más o menos discreta, parece que con el último siglo ha llegado la edad de oro: nadie duda hoy día de la importancia de las matemá...

La política administrativa en el cine español y su vertiente censora

OpenAIRE

Añover Díaz, Rosa

2011-01-01

Para la elaboración de la tesis doctoral se ha vaciado toda la documentación de censura cinematográfica que hemos podido localizar en el archivo central de la administración del ministerio de cultura y en el archivo general de la administración, sección cultura, y en presidencia del gobierno. La documentación la hemos clasificado de acuerdo con el tema objeto de censura y cronológicamente según su presentación a censura previa. Al final se ha llegado a la conclusión de la relación entre el mu...

La política administrativa en el cine español y su vertiente censora

OpenAIRE

Añover Díaz, Rosa

1991-01-01

Para la elaboración de la tesis doctoral se ha vaciado toda la documentación de censura cinematográfica que hemos podido localizar en el archivo central de la administración del ministerio de cultura y en el archivo general de la administración, sección cultura, y en presidencia del gobierno. La documentación la hemos clasificado de acuerdo con el tema objeto de censura y cronológicamente según su presentación a censura previa. Al final se ha llegado a la conclusión de la relación entre el mu...

El bastón de Esculapio: su historia

Directory of Open Access Journals (Sweden)

Isael Armando Pérez Vazquez

Full Text Available Se realizó la revisión bibliográfica con el objetivo de que los jóvenes profesionales de la salud conozcan acerca de la historia, origen y significado del distintivo que representa a la Medicina en todos sus campos con un carácter humanista y elevados valores éticos y morales: el emblema de Esculapio, al que se le atribuyen dotes para calmar o apaciguar. Esta imagen del bastón con la serpiente es la que ha quedado como un atributo del dios curativo y ha llegado hasta la actualidad como símbolo de la Medicina.

Electrospun Porous PDLLA Fiber Membrane Coated with nHA

Directory of Open Access Journals (Sweden)

Linhui Qiang

2018-05-01

Full Text Available Porous poly- D, L-lactic acid (PDLLA electrospinning fiber membrane was prepared, and nano-hydroxyapatite (nHA was adsorbed and wrapped into it during the unique shrinking process of the PDLLA fiber membrane to fabricate the PDLLA/nHA composite membrane scaffold for tissue engineering. Compare with the composite fibers prepared by blend electrospinning, most of nHA particles are observed to distribute on the surface of new type composite fibers, which could significantly improve the water wettability and induce the cellular adherence. FTIR analysis indicated that the PDLLA/nHA composite fibrous membrane was formed by physical adsorption. The combination was probed by scanning electron microscope, thermo-gravimetric, water contact angle and mechanical property analysis. It was proved that the nHA particles’ content and distribution, surface wettability, modulus and tensile strength of PDLLA/nHA composite fibrous membrane were influenced by the concentration of nHA dispersion and pores on the PDLLA fiber surface. The 10.6 wt % PDLLA/nHA composite fibrous membrane exhibits a more balanced tensile strength (3.28 MPa and surface wettability (with a water contact angle of 0° of the composite mats. Scanning electron microscope and confocal laser scanning microscopy images of chondrocyte proliferation further showed that the composite scaffold is non-toxic. The adherence and proliferation of chondrocytes on the 10.6 wt % PDLLA/nHA fibrous membrane was significantly improved, compared with PDLLA mat. The 10.6 wt % PDLLA/nHA composite fibrous membrane has potential application value as scaffold material in tissue engineering.

Preparation of bioactive porous HA/PCL composite scaffolds

Energy Technology Data Exchange (ETDEWEB)

Zhao, J.; Guo, L.Y.; Yang, X.B. [Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031 (China); Weng, J. [Key Laboratory of Advanced Technologies of Materials (Ministry of Education), School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031 (China)], E-mail: jweng@swjtu.cn

2008-12-30

Porous hydroxyapatite (HA) bioceramic scaffold has been widely attracted the attention to act as a three-dimensional (3D) template for cell adhesion, proliferation, differentiation and thus promoting bone and cartilage regeneration because of its osteoinduction. However, the porous bioceramic scaffold is fragile so that it is not suitable to be applied in clinic for bone repair or replacement. Therefore, it is significant to improve the mechanical property of porous HA bioceramics while the interconnected structure is maintained for tissue ingrowth in vivo. In the present research, a porous composite scaffold composed of HA scaffold and polycaprolactone (PCL) lining was fabricated by the method of polymer impregnating to produce HA scaffold coated with PCL lining. Subsequently, the composite scaffolds were deposited with biomimetic coating for improving the bioactivity. The HA/PCL composite scaffolds with improved mechanical property and bioactivity is expected to be a promising bone substitute in tissue engineering applications.

Preparation of bioactive porous HA/PCL composite scaffolds

International Nuclear Information System (INIS)

Zhao, J.; Guo, L.Y.; Yang, X.B.; Weng, J.

2008-01-01

Porous hydroxyapatite (HA) bioceramic scaffold has been widely attracted the attention to act as a three-dimensional (3D) template for cell adhesion, proliferation, differentiation and thus promoting bone and cartilage regeneration because of its osteoinduction. However, the porous bioceramic scaffold is fragile so that it is not suitable to be applied in clinic for bone repair or replacement. Therefore, it is significant to improve the mechanical property of porous HA bioceramics while the interconnected structure is maintained for tissue ingrowth in vivo. In the present research, a porous composite scaffold composed of HA scaffold and polycaprolactone (PCL) lining was fabricated by the method of polymer impregnating to produce HA scaffold coated with PCL lining. Subsequently, the composite scaffolds were deposited with biomimetic coating for improving the bioactivity. The HA/PCL composite scaffolds with improved mechanical property and bioactivity is expected to be a promising bone substitute in tissue engineering applications

Preparation and characterization of bio-composite PEEK/nHA

Science.gov (United States)

Jin, Y. S.; Bian, C. C.; Zhang, Z. Q.; Zhao, Y.; Yang, L.

2017-01-01

PEEK/nHA composite material, with excellent mechanical property as polyetheretherketone (PEEK) and biological activity as hydroxyapatite (HA), has attracted wide attention of medical experts and materials science experts. The addition of hydroxyapatite was the decisive factor for biological activity in PEEK/nHA composite. In this paper, acicular nanohydroxyapatite was prepared by chemical precipitation method with Ca(NO3)2, (NH4)2HPO4 as raw material; PEEK/nHA composite was prepared by solution blending and vacuum sintering method. The composite was characterized with FT-IR, XRD, DSC, TG and mechanical property test. Results showed that the composite has good thermal stability and compressive property when the mass ratio of PEEK to nHA is 10:3; and high nHA content can improve the biological activity of the composite, which can meet the basic requirements for bone tissue engineering scaffold.

Immunomodulatory Effects of Hemagglutinin- (HA- Modified A20 B-Cell Lymphoma Expanded as a Brain Tumor on Adoptively Transferred HA-Specific CD4+ T Cells

Directory of Open Access Journals (Sweden)

Valentin P. Shichkin

2014-01-01

Full Text Available Previously, the mouse A20 B-cell lymphoma engineered to express hemagglutinin (HA antigen (A20HA was used as a systemic tumor model. In this work, we used the A20HA cells as a brain tumor. HA-specific CD4+ T cells were transferred intravenously in a tail vein 5 days after A20HA intracranial inoculation and analyzed on days 2, 9, and 16 after the adoptive transfer by different methods. The transferred cells demonstrated state of activation as early as day 2 after the adoptive transfer and most the of viable HA-specific cells became anergic on day 16. Additionally, symptoms of systemic immunosuppression were observed in mice with massive brain tumors at a late stage of the brain tumor progression (days 20–24 after the A20HA inoculation. Despite that, a deal of HA-specific CD4+ T cells kept the functional activity even at the late stage of A20HA tumor growth. The activated HA-specific CD4+ T cells were found also in the brain of brain-tumor-bearing mice. These cells were still responding to reactivation with HA-peptide in vitro. Our data support an idea about sufficient role of both the tumor-specific and -nonspecific mechanisms inducing immunosuppression in cancer patients.

Esclusa Wilson

Directory of Open Access Journals (Sweden)

Editorial, Equipo

1959-11-01

Full Text Available En la presa Wilson, construida sobre el río Tennessee, ya existía una esclusa y canal que permitía la navegación fluvial por el río. Este rio tiene un curso de difícil navegación en una extensión próxima a la presa, pero la construcción de canales y esclusas ha permitido mejorar estas vías navegables. Estos últimos años ha aumentado tanto la navegación, que la esclusa existente no bastaba para hacer frente al paso de tantas embarcaciones dedicadas al transporte de mercancías que, aún en competencia con él transporte por ferrocarril y carretera, ha llegado a un volumen anual de unos trece millones de toneladas.

Laser treatment of plasma sprayed HA coatings

NARCIS (Netherlands)

Khor, KA; Vreeling, A; Dong, ZL; Cheang, P

1999-01-01

Laser treatment was conducted on plasma sprayed hydroxyapatite (HA) coatings using a Nd-YAG pulse laser. Various laser parameters were investigated. The results showed that the HA surface melted when an energy level of greater than or equal to 2 J and a spot size of 2 mm was employed during

Hyaluronan (HA) interacting proteins RHAMM and hyaluronidase impact prostate cancer cell behavior and invadopodia formation in 3D HA-based hydrogels.

Science.gov (United States)

Gurski, Lisa A; Xu, Xian; Labrada, Lyana N; Nguyen, Ngoc T; Xiao, Longxi; van Golen, Kenneth L; Jia, Xinqiao; Farach-Carson, Mary C

2012-01-01

To study the individual functions of hyaluronan interacting proteins in prostate cancer (PCa) motility through connective tissues, we developed a novel three-dimensional (3D) hyaluronic acid (HA) hydrogel assay that provides a flexible, quantifiable, and physiologically relevant alternative to current methods. Invasion in this system reflects the prevalence of HA in connective tissues and its role in the promotion of cancer cell motility and tissue invasion, making the system ideal to study invasion through bone marrow or other HA-rich connective tissues. The bio-compatible cross-linking process we used allows for direct encapsulation of cancer cells within the gel where they adopt a distinct, cluster-like morphology. Metastatic PCa cells in these hydrogels develop fingerlike structures, "invadopodia", consistent with their invasive properties. The number of invadopodia, as well as cluster size, shape, and convergence, can provide a quantifiable measure of invasive potential. Among candidate hyaluronan interacting proteins that could be responsible for the behavior we observed, we found that culture in the HA hydrogel triggers invasive PCa cells to differentially express and localize receptor for hyaluronan mediated motility (RHAMM)/CD168 which, in the absence of CD44, appears to contribute to PCa motility and invasion by interacting with the HA hydrogel components. PCa cell invasion through the HA hydrogel also was found to depend on the activity of hyaluronidases. Studies shown here reveal that while hyaluronidase activity is necessary for invadopodia and inter-connecting cluster formation, activity alone is not sufficient for acquisition of invasiveness to occur. We therefore suggest that development of invasive behavior in 3D HA-based systems requires development of additional cellular features, such as activation of motility associated pathways that regulate formation of invadopodia. Thus, we report development of a 3D system amenable to dissection of

Reflexiones sobre interpretaciones recientes de la violencia en Colombia

Directory of Open Access Journals (Sweden)

Pedro Valenzuela

2002-01-01

Full Text Available Estudios recientes han negado la relación de causalidad entre las condiciones objetivas y la violencia en Colombia. Este artículo critica la forma como se ha llegado a estas conclusiones y discute algunas hipótesis que vinculan la cultura y las causas objetivas con la violencia. Concluye con una discusión sobre la interpretación de la relación entre causas estructurales y el conflicto armado interno.

Las adicciones como construcción social: conocimientos, posicionamiento público, e implementación estatal de tratamientos

OpenAIRE

Levín, Luciano Guillermo

2011-01-01

Levin, L. G. (2016). Las adicciones como construcción social: conocimientos, posicionamiento público, e implementación estatal de tratamientos (Tesis de posgrado). Universidad Nacional de Quilmes, Bernal, Argentina El estudio recorre los siguientes interrogantes: ¿Cómo ha llegado a predominar la actual estrategia de atención de las adicciones? ¿Cómo se puede entender la ausencia de un tratamiento, manifestada en la diversidad terapéutica actual?. A su vez, teniendo en cuenta...

Sterilisation effect study on granular hydroxyapatite (HA)

International Nuclear Information System (INIS)

Saidu, M.F.; Mashita, M.; Khadijah, K.; Fazan, F.; Khalid, K.A.

2004-01-01

Hydroxyapatite (HA) is a calcium phosphate bio ceramic that has been shown by many authors to be biocompatible with bioactive properties. It is widely accepted as the best synthetic material available for surgical use as a bone graft substitute. HA granules produced by Amerce-SIRIM from local materials underwent 5 types of sterilisation techniques with different ageing periods. Samples were tested for chemical and phase composition and microbial contamination before and after being sterilised. From the microbiological tests done, none of the unsterilised positive control yielded a positive culture. Results from X-Ray diffraction studies found that all the sterilisation techniques did not chemically degrade or structurally change the HA granules significantly. (Author)

El folklore y sus paradojas

Directory of Open Access Journals (Sweden)

HONORIO M. VELASCO MAILLO

1990-01-01

Full Text Available Uno de los rasgos más sobresalientes de la historia del folklore en España y otras naciones europeas son sus paradojas. Propuesto primero como ciencia ha llegado a ser claramente rechazado por posteriores ambientes científicos. Tendría interés hacer una historia social del folklore. Este artículo sugiere que tales paradojas y contradicciones están relacionadas con el paradigma científico que asumieron sus promotores, el evolucionismo cultural y con un concepto idealizado de "pueblo", que ayudaron a construir presentando colecciones de materiales. También analiza las diferentes funciones sociales que ha cumplido el discurso folklórico.

El quijote de cervantes y el quijote de unamuno

OpenAIRE

Buenaventura, Enrique

2010-01-01

"Hay un Quijote de Cervantes, creado por Cervantes, y un Quijote de Unamuno, recreado por Unamuno. y así iremos constantemente de un Quijote a otro Quijote y de un don Miguel a otro don Miguel. Bastantes, innumerables interpretaciones se han hecho de Don Quijote y ya se ha llegado desde la interpretación meramente idealista a la más realista y hasta materialista histórica. Entre todas ellas se encuentra la sentida, viva y recreadora de Unamuno".

Turismo comunitario y desarrollo humano y sostenible en el Agroparque Los Soches: evaluación de una experiencia a través del estudio de casos

OpenAIRE

Navas Camargo, Johanna Fernanda

2015-01-01

El Turismo Comunitario se ha configurado por parte de diferentes entidades multilaterales y gubernamentales, como una actividad que, según ellos, puede contribuir a la atenuación de la pobreza e impulsar el desarrollo. Sin embargo, ciertas voces han llegado a oponerse a esta práctica por considerarla un mecanismo en donde los realmente beneficiados no son las Comunidades, como por principio debería serlo, sino terceras partes. Otras tantas, siguen apoyando esta práctica en crecimiento y respa...

Enantiomers of HA-966 (3-amino-1-hydroxypyrrolid-2-one) exhibit distinct central nervous system effects: (+)-HA-966 is a selective glycine/N-methyl-D-aspartate receptor antagonist, but (-)-HA-966 is a potent gamma-butyrolactone-like sedative

International Nuclear Information System (INIS)

Singh, L.; Donald, A.E.; Foster, A.C.; Hutson, P.H.; Iversen, L.L.; Iversen, S.D.; Kemp, J.A.; Leeson, P.D.; Marshall, G.R.; Oles, R.J.; Priestley, T.; Thorn, L.; Tricklebank, M.D.; Vass, C.A.; Williams, B.J.

1990-01-01

The antagonist effect of ±-3-amino-1-hydroxypyrrolid-2-one (HA-966) at the N-methyl-D-aspartate (NMDA) receptor occurs through a selective interaction with the glycine modulatory site within the receptor complex. When the enantiomers of ±-HA-966 were resolved, the (R)-(+)-enantiomer was found to be a selective glycine/NMDA receptor antagonist, a property that accounts for its anticonvulsant activity in vivo. In contrast, the (S)-(-)-enantiomer was only weakly active as an NMDA-receptor antagonist, but nevertheless it possessed a marked sedative and muscle relaxant action in vivo. In radioligand binding experiments, (+)-HA-966 inhibited strychnine-insensitive [ 3 H]glycine binding to rat cerebral cortex synaptic membranes with an IC 50 of 12.5 μM, whereas (-)-HA-966 had an IC 50 value of 339 μM. In mice, (+)-HA-966 antagonized sound and N-methyl-DL-aspartic acid (NMDLA)-induced seizures. The coadministration of D-serine dose-dependently antagonized the anticonvulsant effect of a submaximal dose of (+)-HA-966 against NMDLA-induced seizures. The sedative/ataxic effect of racemic HA-966 was mainly attributable to the (-)-enantiomer. It is suggested that, as in the case of the sedative γ-butyrolactone, disruption of striatal dopaminergic mechanisms may be responsible for this action

Solar and electric automobiles: An idea whose time has come; Automoviles solares y electricos: Una idea cuyo tiempo ha llegado

Energy Technology Data Exchange (ETDEWEB)

Padilla, Beatriz [Academia de la Investigacion Cientifica, A. C., Mexico, D. F. (Mexico)

1993-12-31

Given the dependence of our transport system in combustible hydrocarbons that, besides generating serious pollution levels, eventually will become scarce, severely affecting the world economy- it is compulsory to develop non polluting transportation, capable of operating with alternative energies. In the avant-guard of this technological achievement are the competition solar automobiles, that have made great contributions (particularly for the rigorous application of the design for efficiency principles) in the development of the applicable technology to the electric vehicles. Tonatiuh is the first competition solar car built in Mexico. In 1993 Tonatiuh will compete in the Rally Formula Sol in Mexico and in the World Solar of Australia. A superficial comparison of electric vehicles with vehicles equipped with internal combustion motors, demonstrates the great advantages of the first ones, most of all at the light of the current technological advances propitiated by laws that demand them for the sale of electric vehicles. This incipient industry presents a great opportunity for Mexico, since the markets are not saturated and exist large areas of research and development. For improvement of the air quality in Mexico city, the electric vehicle would have a very positive impact, mostly applied to public transportation. [Espanol] Dada la dependencia de nuestro sistema de transporte en hidrocarburos combustibles que, ademas de generar graves niveles de contaminacion eventualmente escacearan, afectando severamente la economia mundial - es imperativo desarrollar transportes no contaminantes capaces de operar con energias alternativas. En la vanguardia de esta avanzada tecnologia se encuentran los autos solares de carreras, los cuales han hecho grandes aportaciones (particularmente por la aplicacion rigurosa de los principios del diseno para eficiencia) el desarrollo de tecnologia aplicable a vehiculos electricos. Tonatiuh es el primer auto solar de carreras que se construye en Mexico. En 1993 Tonatiuh competira en la carrera Formula Sol en Mexico y en el World Solar Challenge de Australia. Una comparacion somera de vehiculos electricos con motores de combustion interna, demuestra las grandes ventajas de los primeros, sobre todo a la luz de los actuales avances tecnologicos propiciados por leyes que exigen la venta de vehiculos electricos. Esta naciente industria presenta una gran oportunidad para Mexico, ya que los mercados no estan saturados y existen amplias areas de investigacion y desarrollo. Para mejorar la calidad del aire en la ciudad de Mexico, los vehiculos electricos tendrian un impacto muy positivo, sobre todo aplicados en el transporte publico.

Solar and electric automobiles: An idea whose time has come; Automoviles solares y electricos: Una idea cuyo tiempo ha llegado

Energy Technology Data Exchange (ETDEWEB)

Padilla, Beatriz [Academia de la Investigacion Cientifica, A. C., Mexico, D. F. (Mexico)

1992-12-31

Given the dependence of our transport system in combustible hydrocarbons that, besides generating serious pollution levels, eventually will become scarce, severely affecting the world economy- it is compulsory to develop non polluting transportation, capable of operating with alternative energies. In the avant-guard of this technological achievement are the competition solar automobiles, that have made great contributions (particularly for the rigorous application of the design for efficiency principles) in the development of the applicable technology to the electric vehicles. Tonatiuh is the first competition solar car built in Mexico. In 1993 Tonatiuh will compete in the Rally Formula Sol in Mexico and in the World Solar of Australia. A superficial comparison of electric vehicles with vehicles equipped with internal combustion motors, demonstrates the great advantages of the first ones, most of all at the light of the current technological advances propitiated by laws that demand them for the sale of electric vehicles. This incipient industry presents a great opportunity for Mexico, since the markets are not saturated and exist large areas of research and development. For improvement of the air quality in Mexico city, the electric vehicle would have a very positive impact, mostly applied to public transportation. [Espanol] Dada la dependencia de nuestro sistema de transporte en hidrocarburos combustibles que, ademas de generar graves niveles de contaminacion eventualmente escacearan, afectando severamente la economia mundial - es imperativo desarrollar transportes no contaminantes capaces de operar con energias alternativas. En la vanguardia de esta avanzada tecnologia se encuentran los autos solares de carreras, los cuales han hecho grandes aportaciones (particularmente por la aplicacion rigurosa de los principios del diseno para eficiencia) el desarrollo de tecnologia aplicable a vehiculos electricos. Tonatiuh es el primer auto solar de carreras que se construye en Mexico. En 1993 Tonatiuh competira en la carrera Formula Sol en Mexico y en el World Solar Challenge de Australia. Una comparacion somera de vehiculos electricos con motores de combustion interna, demuestra las grandes ventajas de los primeros, sobre todo a la luz de los actuales avances tecnologicos propiciados por leyes que exigen la venta de vehiculos electricos. Esta naciente industria presenta una gran oportunidad para Mexico, ya que los mercados no estan saturados y existen amplias areas de investigacion y desarrollo. Para mejorar la calidad del aire en la ciudad de Mexico, los vehiculos electricos tendrian un impacto muy positivo, sobre todo aplicados en el transporte publico.

Preparation, optimization and property of PVA-HA/PAA composite hydrogel.

Science.gov (United States)

Chen, Kai; Liu, Jinlong; Yang, Xuehui; Zhang, Dekun

2017-09-01

PVA-HA/PAA composite hydrogel is prepared by freezing-thawing, PEG dehydration and annealing method. Orthogonal design method is used to choose the optimization combination. Results showed that HA and PVA have the maximum effect on water content. PVA and freezing-thawing cycles have the maximum effect on creep resistance and stress relaxation rate of hydrogel. Annealing temperature and freezing-thawing cycles have the maximum effect on compressive elastic modulus of hydrogel. Comparing with the water content and mechanical properties of 16 kinds of combination, PVA-HA/PAA composite hydrogel with freezing-thawing cycles of 3, annealing temperature of 120°C, PVA of 16%, HA of 2%, PAA of 4% has the optimization comprehensive properties. PVA-HA/PAA composite hydrogel has a porous network structure. There are some interactions between PVA, HA and PAA in hydrogel and the properties of hydrogel are strengthened. The annealing treatment improves the crystalline and crosslinking of hydrogel. Therefore, the annealing PVA-HA/PAA composite hydrogel has good thermostability, strength and mechanical properties. It also has good lubrication property and its friction coefficient is relative low. Copyright © 2017 Elsevier B.V. All rights reserved.

[Construction of porous hydroxyapatite (HA) block loaded with cultured chondrocytes].

Science.gov (United States)

Yan, M; Dang, G

1999-07-01

To construct a kind of bone healing enhancing implant with cultured chondrocytes bound to hydroxyapatite (HA). Chondrocytes were obtained from the costicartilage of rat and were cultured on the porous HA blocks, 3 mm x 3 mm x 4 mm size, for three and seven days. Scanning electron micrograph was taken to show whether the cells grew outside and inside the pore of HA block. The cells cultured on tiny glass sheet for 2 days were used to prove where the cells come from by in situ hybridization technique with alpha1 (II) cDNA probe. Scanning electron micrographs showed that the pores of the HA surface and inside of the blocks are filled with cultured cells, especially the longer cultured block. The cells were chondrocytes confirmed by in situ hybridization. The porous HA can be used as cell cultured substrate and chondrocyte can adhere and proliferate inside the porous HA block.

Võidupüha 8. mail? / Leonti Kährik

Index Scriptorium Estoniae

Kährik, Leonti

2005-01-01

Eesti peaks väärikalt tähistama võidupüha kui euroopalike demokraatlike väärtuste võitu natsismi ja fašismi üle. Lähtudes 1940. a. juunini Eestis kehtinud vööndiajast, võiks võidupüha tähistada 8. mail

Análisis de casos con TIC en la formación inicial del conocimiento profesional experto del profesorado

Directory of Open Access Journals (Sweden)

Teresa MAURI

2006-01-01

Full Text Available La reforma propiciada por las directrices de Bolonia supone un desafío importante para la educación superior, que ha traído como consecuencia la puesta en marcha de numerosas propuestas de innovación docente. Una de ellas es la construcción del conocimiento experto, que resulta particularmente importante en el ámbito de la formación del profesorado. La experiencia de innovación que presentamos ha demostrado ser muy potente para la elaboración de contextos de práctica que facilitan al alumnado el uso funcional del conocimiento y el desarrollo de las competencias propias de su comunidad profesional. Esta experiencia consiste en el uso de una metodología docente basada en casos y mediada por la plataforma virtual Moodle. La actividad del alumno se convierte progresivamente en experta gracias a que cuenta con la mediación de ayudas educativas diferentes que el profesor facilita con el uso de las TIC.

Radioactividad natural y salud: ¿ha llegado el momento de prestarle más atención? Natural radioactivity and health: is it the moment to pay more attention?

Directory of Open Access Journals (Sweden)

Enrique Estrada Vélez

2008-12-01

Full Text Available En la actualidad es frecuente que se tengan noticias de problemas de salud que en el pasado no se conocían o no eran preferentes. Hemos superado enfermedades prioritarias y la esperanza de vida ha aumen- tado, lo que aconseja empezar a prestar una mayor atención a la radioactividad natural por incidir en la oncogénesis y la teratogenicidad y, por otro lado, estar vinculada a factores y procesos nuevos como los edificios inteligentes en los que está implicado el radón por un lado y, sobre todo, a exposición debida a la explotación del agua fósil por otro. La radioactividad natural no origina procesos agudos, siempre está ligada a procesos crónicos, en los que el daño incide en los sistemas de información genética: el ADN como molécula individual y el cromosoma como un todo, dando origen a procesos oncológicos, por lo que es un cancerígeno per se de gran importancia. En este artículo se hace una llamada de atención sobre la implicación de la radioactividad natural en los edificios, en el agua, etc., de ciertas zonas geográficas con altos niveles de radioactividad natural.At the moment, it is more usual to be aware of health problems, which were not given priority in the past .We, have overcome previous health problems to which we have given importance and life expectancy has increased and inconsequence we do now require to give more attention to natural radioactivity because it affects oncological and genetic problems. On the one hand, it is connected with new processes or factors regarding, for example building techniques and materials which include radon and on the other hand, to exposure by exploitation to fossil water. Natural radioactivity does not produce acute illness but it is always linked to chronic illness, in which to damage affects the genetic information: the AND as a simple molecule and the chromosome as a whole, which gives rise to oncological processes. Therefore natural radioactivity is a cancer producing

Prospects of HA-Based Universal Influenza Vaccine

Directory of Open Access Journals (Sweden)

Anwar M. Hashem

2015-01-01

Full Text Available Current influenza vaccines afford substantial protection in humans by inducing strain-specific neutralizing antibodies (Abs. Most of these Abs target highly variable immunodominant epitopes in the globular domain of the viral hemagglutinin (HA. Therefore, current vaccines may not be able to induce heterosubtypic immunity against the divergent influenza subtypes. The identification of broadly neutralizing Abs (BnAbs against influenza HA using recent technological advancements in antibody libraries, hybridoma, and isolation of single Ab-secreting plasma cells has increased the interest in developing a universal influenza vaccine as it could provide life-long protection. While these BnAbs can serve as a source for passive immunotherapy, their identification represents an important step towards the design of such a universal vaccine. This review describes the recent advances and approaches used in the development of universal influenza vaccine based on highly conserved HA regions identified by BnAbs.

Los estudios de pedagogía en Europa en el contexto de la implantación del proceso de Bolonia y la situación de la Educación Comparada

Directory of Open Access Journals (Sweden)

Joan María Senent Sánchez

2005-01-01

Full Text Available El artículo nos presenta un análisis comparativo de la situación de los estudios superiores de carácter pedagógico en 23 países de Europa que han sido agrupados en 5 bloques: a Países anglofónos o de influencia británica: Reino Unido, Irlanda y Malta. b Escandinavia: Noruega, Finlandia, Dinamarca y Holanda. c Países de lengua o influencia alemana: Alemania, Austria y Eslovenia. d Europa del Este: República Checa, Eslovaquia y Polonia. e Países francófonos: Francia, Belgica, Luxemburgo y Suiza romance. . f Países meridionales: Portugal, España, Italia, Grecia y Chipre. El análisis se basa en la presencia de los estudios pedágógicos en los "grados" y "postgrados" de ese país, la estructura adoptada, las características del del programa de formación y su situación respecto a la adaptación al proceso de Bolonia. El artículo termina con la presentación de las conclusiones del informe, así como un breve análisis de la situación de la Educación Comparada en las universidades europeas, tanto como disciplina que como método de trabajo.

¿Fue una meningitis tuberculosa terminal la causa de muerte de Simón Bolívar?

Directory of Open Access Journals (Sweden)

Fernando Guzmán Mora

2014-09-01

Full Text Available Simón Bolívar. Mucho se ha especulado sobre la causa de su uerte. Algunos han llegado al extremo de acusar a sus propios médicos de haber administrads medicamentos equivocados para los conocimientos de aquel entonces. Otros han mencionado la malaria como la enfermedad que causó su deceso. Las opiniones no son iguales y encuentran particular divergencia tres teorías sobre la causa última de su muerte: El paludismo, el absceso hepático amebiano y la tuberculosis.

Creación de guías prácticas para incrementar la motivación en una organización y aplicación de una de ellas en la empresa FARCOMED S.A. de la ciudad de Cuenca

OpenAIRE

Cabrera Cabrera, Mónica Patricia; Sánchez Orellana, Mayra Alexandra

2008-01-01

Hoy en día las organizaciones enfrentan un gran reto, el mantener motivados a sus colaboradores, muchos han sido los intentos y las investigaciones en torno al tema pero no se ha llegado a definir un modelo perfecto de motivación a los colaboradores organizacionales, entre otros factores esto se debe a que todos los seres humanos somos diferentes y por ende cada uno tenemos diferentes necesidades por satisfacer. Es por ello que consideramos a la observación como una herramienta muy import...

スペインの移民に対する言語教育

OpenAIRE

江澤, 照美; エザワ, テルミ; Terumi, EZAWA

2006-01-01

Espana ha sido un pais al que han llegado a vivir muchos inmigrantes de diversos paises. Muchos de ellos han venido de Africa, Asia, Europa, los cuales no son nativos del espanol. Y el resto de Latinoamerica. Entre los espanoles hay algunos que no quieren aceptarlos y otros que lo hacen con gusto. Todavia quedan algunos prejuicios discriminatorios sobre los inmigrantes, pero, va a desapareciendo poco a poco el sentimiento malo de los espanoles hacia ellos. El Gobierno se ve obligado a tomar m...

Desarrollo de un sistema de reconocimiento de emociones faciales en tiempo real

OpenAIRE

Ribes Gil, Héctor

2017-01-01

Una organización del trastorno nutricional ha puesto en práctica un nuevo tratamiento para sus pacientes. Éste está directamente relacionado con la emoción del paciente, cuya emoción se clasifica oralmente. Llegados a ese punto aparece el reto de automatizar esta tarea, desarrollando un sistema de reconocimiento de emociones faciales en tiempo real caracterizado por un detector de rostros, un detector de Key Points y una red neuronal convolucional utilizando "DeepLearning" para extraer las ca...

Imagen narrativa: De la imagen prehistórica a las tecnologías de la imagen

Directory of Open Access Journals (Sweden)

Vicente Peña Timón

2012-04-01

Full Text Available El artículo está estructurado en dos partes. La primera, titulada La narración icónica en la prehistoria, explica el porqué, ya en aquella época, existían "narraciones", donde "alguien" contaba "algo" acerca de "otro alguien", ocurrido en "algún sitio", en un "momento dado". También se expone cómo esta construcción narrativa es el inicio de lo que se ha llegado a denominar imagen narrativa. En la segunda parte, titulada Perspectiva histórica de la imagen narrativa, se realiza un sucinto recorrido histórico,  para poner de manifiesto el protagonismo que siempre ha revelado la Imagen en el devenir y desarrollo de los Pueblos.

Bases para proyectiles dirigidos

Directory of Open Access Journals (Sweden)

Editorial, Equipo

1959-03-01

Full Text Available Aunque actualmente no se ha llegado a una línea general de métodos o sistemas que gobiernen un tipo característico de rampa y servicios auxiliares necesarios para el lanzamiento al espacio de proyectiles dirigidos a grandes alturas y distancias, las experiencias obtenidas en diferentes ensayos, utilizando distintos tipos de proyectiles y trayectorias balísticas, han sentado toda una serie de procedimientos, datos y conclusiones de gran valor balístico que, aun teniendo en cuenta la continua evolución del proyectil, sus formas, combustibles y alcances, se conocen ya, con bastante aproximación, las condiciones mínimas que ha de reunir una base dedicada a este tipo de lanzamientos.

La planificación por competencias en la reforma de Bolonia de la educación superior: un análisis crítico/A planificação por competências a reforma de Bolonha da educação superior: uma análise crítica

Directory of Open Access Journals (Sweden)

Antonio Bolívar

2007-01-01

Full Text Available Este trabajo analiza la dimensión curricular y didáctica, más que la estructural, de la reforma de Bolonia. Se describen las principales líneas y orientaciones de la planificación del currículum y la enseñanza según el Proceso de Convergencia de Bolonia, pensando que puede ofrecer una oportunidad institucional para mejorar la docencia en la Universidad, a condición de no limitarse a cambios estructurales, para lo que debe incidir en mudar la propia mentalidad y cultura establecida. Más particularmente, se realiza un análisis crítico del papel que juegan las competencias en el proceso de planificación, tanto en el modelo adoptado (Proyecto Tuning, como por la subordinación al perfil profesional y su vinculación con el mercado laboral. Desde un abordaje crítico se defiende que, si bien la Universidad debe preparar para el ejercicio profesional, ello no puede suponer desdeñar otras dimensiones de la Educación Superior. Este trabalho analisa a dimensão curricular e didática, mais do que a estrutural, da reforma de Bolonha. Descrevem-se as principais linhas e orientações da planificação do currículo e do conhecimento segundo o Processo de Convergência de Bolonha, pensando que se pode oferecer uma oportunidade institucional para melhorar a docência da Universidade. Para que isto aconteça, a condição é de que o processo não se limite a câmbios estruturais, mas sim o que deve incidir em mudar a própria mentalidade e cultura estabelecida. Mais particularmente, se realiza uma analise critica do papel que se julgam as competências do processo de planificação, tanto no modelo adaptado (Prometo Tuning, como pela subordinação do perfil profissional, e sua vinculação com o Mercado de Trabalho. Desde uma abordagem critica se defende que a Universidade deve preparar para o exercício profissional, e isto não pode supor desdenhar outras dimensões da Educação Superior.

Crystal structure of the HA3 subcomponent of Clostridium botulinum type C progenitor toxin.

Science.gov (United States)

Nakamura, Toshio; Kotani, Mao; Tonozuka, Takashi; Ide, Azusa; Oguma, Keiji; Nishikawa, Atsushi

2009-01-30

The Clostridium botulinum type C 16S progenitor toxin contains a neurotoxin and several nontoxic components, designated nontoxic nonhemagglutinin (HA), HA1 (HA-33), HA2 (HA-17), HA3a (HA-22-23), and HA3b (HA-53). The HA3b subcomponent seems to play an important role cooperatively with HA1 in the internalization of the toxin by gastrointestinal epithelial cells via binding of these subcomponents to specific oligosaccharides. In this study, we investigated the sugar-binding specificity of the HA3b subcomponent using recombinant protein fused to glutathione S-transferase and determined the three-dimensional structure of the HA3a-HA3b complex based on X-ray crystallography. The crystal structure was determined at a resolution of 2.6 A. HA3b contains three domains, domains I to III, and the structure of domain I resembles HA3a. In crystal packing, three HA3a-HA3b molecules are assembled to form a three-leaved propeller-like structure. The three HA3b domain I and three HA3a alternate, forming a trimer of dimers. In a database search, no proteins with high structural homology to any of the domains (Z score >10) were found. Especially, HA3a and HA3b domain I, mainly composed of beta-sheets, reveal a unique fold. In binding assays, HA3b bound sialic acid with high affinity, but did not bind galactose, N-acetylgalactosamine, or N-acetylglucosamine. The electron density of liganded N-acetylneuraminic acid was determined by crystal soaking. In the sugar-complex structure, the N-acetylneuraminic acid-binding site was located in the cleft formed between domains II and III of HA3b. This report provides the first determination of the three-dimensional structure of the HA3a-HA3b complex and its sialic acid binding site. Our results will provide useful information for elucidating the mechanism of assembly of the C16S toxin and for understanding the interactions with oligosaccharides on epithelial cells and internalization of the botulinum toxin complex.

Sr-containing hydroxyapatite: morphologies of HA crystals and bioactivity on osteoblast cells

Energy Technology Data Exchange (ETDEWEB)

Aina, Valentina [Department of Chemistry, Università degli Studi di Torino, Via P. Giuria 7, 10125 Torino (Italy); Centre of Excellence NIS (Nanostructured Interfaces and Surface) Università degli Studi di Torino (Italy); INSTM (Italian National Consortium for Materials Science and Technology), UdR Università di Torino (Italy); Bergandi, Loredana, E-mail: loredana.bergandi@unito.it [Department of Oncology, Università degli Studi di Torino, Via Santena 5/bis, 10126 Torino (Italy); Lusvardi, Gigliola; Malavasi, Gianluca [Department of Chemical and Geological Sciences, Università di Modena and Reggio Emilia, Via Campi 183, 41125 Modena (Italy); Imrie, Flora E.; Gibson, Iain R. [School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD (United Kingdom); Cerrato, Giuseppina [Department of Chemistry, Università degli Studi di Torino, Via P. Giuria 7, 10125 Torino (Italy); Centre of Excellence NIS (Nanostructured Interfaces and Surface) Università degli Studi di Torino (Italy); INSTM (Italian National Consortium for Materials Science and Technology), UdR Università di Torino (Italy); Ghigo, Dario [Department of Oncology, Università degli Studi di Torino, Via Santena 5/bis, 10126 Torino (Italy)

2013-04-01

A series of Sr-substituted hydroxyapatites (HA), of general formula Ca{sub (10−x)}Sr{sub x}(PO{sub 4}){sub 6}(OH){sub 2}, where x = 2 and 4, were synthesized by solid state methods and characterized extensively. The reactivity of these materials in cell culture medium was evaluated, and the behavior towards MG-63 osteoblast cells (in terms of cytotoxicity and proliferation assays) was studied. Future in vivo studies will give further insights into the behavior of the materials. A paper by Lagergren et al. (1975), concerning Sr-substituted HA prepared by a solid state method, reports that the presence of Sr in the apatite composition strongly influences the apatite diffraction patterns. Zeglinsky et al. (2012) investigated Sr-substituted HA by ab initio methods and Rietveld analyses and reported changes in the HA unit cell volume and shape due to the Sr addition. To further clarify the role played by the addition of Sr on the physico-chemical properties of these materials we prepared Sr-substituted HA compositions by a solid state method, using different reagents, thermal treatments and a multi-technique approach. Our results indicated that the introduction of Sr at the levels considered here does influence the structure of HA. There is also evidence of a decrease in the crystallinity degree of the materials upon Sr addition. The introduction of increasing amounts of Sr into the HA composition causes a decrease in the specific surface area and an enrichment of Sr-apatite phase at the surface of the samples. Bioactivity tests show that the presence of Sr causes changes in particle size and/or morphology during soaking in MEM solution; on the contrary the morphology of pure HA does not change after 14 days of reaction. The presence of Sr, as Sr-substituted HA and SrCl{sub 2,} in cultures of human MG-63 osteoblasts did not produce any cytotoxic effect. In fact, Sr-substituted HA increased the proliferation of osteoblast cells and enhanced cell differentiation: Sr in

Sr-containing hydroxyapatite: morphologies of HA crystals and bioactivity on osteoblast cells

International Nuclear Information System (INIS)

Aina, Valentina; Bergandi, Loredana; Lusvardi, Gigliola; Malavasi, Gianluca; Imrie, Flora E.; Gibson, Iain R.; Cerrato, Giuseppina; Ghigo, Dario

2013-01-01

A series of Sr-substituted hydroxyapatites (HA), of general formula Ca (10−x) Sr x (PO 4 ) 6 (OH) 2 , where x = 2 and 4, were synthesized by solid state methods and characterized extensively. The reactivity of these materials in cell culture medium was evaluated, and the behavior towards MG-63 osteoblast cells (in terms of cytotoxicity and proliferation assays) was studied. Future in vivo studies will give further insights into the behavior of the materials. A paper by Lagergren et al. (1975), concerning Sr-substituted HA prepared by a solid state method, reports that the presence of Sr in the apatite composition strongly influences the apatite diffraction patterns. Zeglinsky et al. (2012) investigated Sr-substituted HA by ab initio methods and Rietveld analyses and reported changes in the HA unit cell volume and shape due to the Sr addition. To further clarify the role played by the addition of Sr on the physico-chemical properties of these materials we prepared Sr-substituted HA compositions by a solid state method, using different reagents, thermal treatments and a multi-technique approach. Our results indicated that the introduction of Sr at the levels considered here does influence the structure of HA. There is also evidence of a decrease in the crystallinity degree of the materials upon Sr addition. The introduction of increasing amounts of Sr into the HA composition causes a decrease in the specific surface area and an enrichment of Sr-apatite phase at the surface of the samples. Bioactivity tests show that the presence of Sr causes changes in particle size and/or morphology during soaking in MEM solution; on the contrary the morphology of pure HA does not change after 14 days of reaction. The presence of Sr, as Sr-substituted HA and SrCl 2, in cultures of human MG-63 osteoblasts did not produce any cytotoxic effect. In fact, Sr-substituted HA increased the proliferation of osteoblast cells and enhanced cell differentiation: Sr in HA has a positive effect

Neuronas Espejo: un nuevo camino dentro de las Neurociencias : Aportes y aplicaciones, en el área de la reeducación y la rehabilitación

OpenAIRE

Figueroa Cuadrado, Emiliano

2013-01-01

El presente trabajo, pretende sumergirse en el mundo de las Neuronas Espejo. Dichas neuronas fueron descubiertas por un equipo de Neurobiólogos Italianos, de la Universidad de Parma, en el año 1995. Es el ultimo "gran descubrimiento" que se ha hecho en el ámbito de las Neurociencias; tanto que hasta algunos especialistas han llegado a decir, que tal descubrimiento estaba llamado a desempeñar en las Neurociencias, un papel semejante al que había tenido en Biología, la decodificación de la e...

Una nueva mirada sobre Rafael del Olmo, a partir de los papeles de Torner conservados en la Biblioteca Tomas Navarro Tomás del CSIC (Madrid

Directory of Open Access Journals (Sweden)

Cristina Martí Martínez

2014-01-01

Estas partituras se presentan en este texto. Sin profundizar en todo su contenido, sí se dará noticia del nexo común a todas ellas: el compositor, de origen cordobés y malagueño de adopción, Rafael del Olmo y Bueno (1826-, que copió la mayoría de las piezas y de cuya breve obra nos ha llegado una zarzuela, algunos arreglos y varios villancicos para coro y banda con poesías del dramaturgo Ramón Franquelo Martínez (1824-1874.

Ortodoncia estética invisible

OpenAIRE

Chávez Sevillano, Manuel Gustavo; Soldevilla Galarza, Luciano

2014-01-01

El adulto suele ofrecer resistencia a los convencionales tratamientos ortodóncicos, debido a la necesidad de llevar brackets visibles, tanto metálicos como estéticos de porcelana. El concepto de Ortodoncia Estética Invisible u Ortodoncia Lingual cubre las expectativas de este tipo de pacientes. La técnica multibrackets con aparatología lingual tiene aproximadamente 25 años de desarrollo y con la experiencia de los casos tratados, se ha llegado a la concepción de una técnica completamente prot...

El Docente/Activista: Una introducción al conocimiento, poder y educación

OpenAIRE

Apple, Michael W.

2015-01-01

Este ensayo fue extraído de Michael W. Apple, Knowledge, Power, and Education: The Selective Works of Michael W. Apple, publicado en Nueva York por la editorial Routledge en 2013. Traducido por Guillermina Belavi Es un artículo para la comprensión de una trayectoria vital comprometida. Mike Apple hace un relato biográfico acerca de cómo ha llegado a ser el docente activista que es. Esta narrativa comienza en el momento que él se incorpora a la Facultad de Educación, como estudiante. Ahonda...

Roman mortars used in the archaeological sites in Spain and Turkey : a comparative study and the design of repair mortars

OpenAIRE

Ergenç, Duygu

2017-01-01

La calidad de los morteros romanos destaca, por lo general, por su alta durabilidad habiendo llegado, después de haber estado puesto más de dos milenios en obra, en muy buenas condiciones de conservación. La caracterización de estos morteros nos va a permitir entender su comportamiento y establecer las pautas para nuevos diseños de morteros que aproximen sus propiedades a las de los morteros romanos. El área de investigación en ciencias del patrimonio cultural ha descubierto hace tiempo estas...

Análisis crítico de la viabilidad del fracking en España

OpenAIRE

Serigot Castellet, Juan

2015-01-01

Las conclusiones a las que se ha llegado en este TFM son las siguientes: -No hay una información precisa y fiable sobre los efectos que puede provocar la parte del fluido inyectado que no es recuperado -En materia de hidrocarburos convencionales, España es un país semi-explorado. Hecho que surge al comparar con otros países europeos cualquiera de los ratios utilizados en la industria, tales como número de sondeos perforados por de superficie sedimentaria ó número km de líneas sísmica po...

Evaluación de los estereotipos negativos hacia la vejez en jóvenes y adultos

OpenAIRE

Menendez Alvarez-Dardet, Susana; Cuevas Toro, Ana María; Pérez Padilla, Javier; Lorence Lara, Bárbara

2016-01-01

Introducción: Los estereotipos negativos hacia el envejecimiento y la vejez pueden influir negativamente en la relación y el trabajo con las personas mayores. El CENVE es una herramienta diseñada para evaluar este tipo de estereotipos viejistas, pero su estructura factorial no ha sido evaluada con jóvenes y adultos. En este trabajo se analiza la estructura factorial del CENVE con sujetos que aún no han llegado a la vejez, y se examinan las relaciones de los estereotipos edadistas con diversos...

Análisis, diseño y aplicación de modelos de diálogo flexibles, contextuales y dinámicos basados en redes bayesianas

OpenAIRE

Fernández Martínez, Fernando

2008-01-01

Resumen El habla es el medio de comunicación natural entre personas. Como evolución de todo lo aprendido durante prácticamente medio siglo de investigación en tecnología del habla, ha llegado el momento de diseñar sistemas que hagan uso de la capacidad comunicativa del habla, aunque esta vez con máquinas como interlocutoras. En ese sentido creemos que es fundamental incorporar al diseño de tales sistemas las ideas recogidas bajo el concepto de “inteligencia ambiental”, ofreciendo así, interfa...

La comunicación (más allá de la ruptura cuando fallan las relaciones: Los limites de poder entre Irán, Israel, EEUU y la cuestión nuclear

Directory of Open Access Journals (Sweden)

José Ignacio Castro Torres

2013-12-01

Full Text Available Las negociaciones entre Irán y la Comunidad Internacional en materia nuclear han resultado desalentadoras. Es ya un hecho que Irán ha llegado a producir energía nucleoeléctrica, aunque existen serias sospechas de que tras el programa nuclear civil se encubra otro militar. En paralelo al avance iraní en materia nuclear se han producido una serie de incidentes que marcan los límites del poder que Irán, Israel y otros actores ejercen en la región y que tienen repercusiones mundiales

Diferenciación sexual y teorías reproductivas en època clásica

OpenAIRE

García-González, José Antonio

2009-01-01

El tema de la reproducción humana y todas aquellas cuestiones vinculadas a él, como la herencia, determinación del sexo, papel desarrollado por los progenitores o la naturaleza y procedencia del semen han formado parte del campo de estudio de los científicos griegos desde sus inicios, como se deja ver en la información que de estas primeras etapas de la ciencia jonia nos ha llegado. Algunas de las respuestas ofrecidas a estas cuestiones prefiguran las teorías actuales. The topi...

HVOF-Sprayed Nano TiO2-HA Coatings Exhibiting Enhanced Biocompatibility

Science.gov (United States)

Lima, R. S.; Dimitrievska, S.; Bureau, M. N.; Marple, B. R.; Petit, A.; Mwale, F.; Antoniou, J.

2010-01-01

Biomedical thermal spray coatings produced via high-velocity oxy-fuel (HVOF) from nanostructured titania (n-TiO2) and 10 wt.% hydroxyapatite (HA) (n-TiO2-10wt.%HA) powders have been engineered as possible future alternatives to HA coatings deposited via air plasma spray (APS). This approach was chosen due to (i) the stability of TiO2 in the human body (i.e., no dissolution) and (ii) bond strength values on Ti-6Al-4V substrates more than two times higher than those of APS HA coatings. To explore the bioperformance of these novel materials and coatings, human mesenchymal stem cells (hMSCs) were cultured from 1 to 21 days on the surface of HVOF-sprayed n-TiO2 and n-TiO2-10 wt.%HA coatings. APS HA coatings and uncoated Ti-6Al-4V substrates were employed as controls. The profiles of the hMSCs were evaluated for (i) cellular proliferation, (ii) biochemical analysis of alkaline phosphatase (ALP) activity, (iii) cytoskeleton organization (fluorescent/confocal microscopy), and (iv) cell/substrate interaction via scanning electron microscopy (SEM). The biochemical analysis indicated that the hMSCs cultured on n-TiO2-10 wt.%HA coatings exhibited superior levels of bioactivity than hMSCs cultured on APS HA and pure n-TiO2 coatings. The cytoskeleton organization demonstrated a higher degree of cellular proliferation on the HVOF-sprayed n-TiO2-10wt.%HA coatings when compared to the control coatings. These results are considered promising for engineering improved performance in the next generation of thermally sprayed biomedical coatings.

La tutoría y la orientación universitaria en la nueva coyuntura de la enseñanza superior : el programa "Velero"

Directory of Open Access Journals (Sweden)

Pedro Ricardo Alvarez Pérez

2013-05-01

Full Text Available Este artículo aborda el tema de la tutoría universitaria, enmarcándola en la coyuntura actual de la enseñanza superior, donde se vive una sucesión casi continua de movimientos y cambios que emanan básicamente de los acuerdos de Bolonia. En este mar de turbulencias, parece que una de las tendencias a seguir apunta al énfasis que quiere ponerse en los procesos de aprendizaje del alumnado y en la labor tutorial que debe realizar el profesorado universitario. Para facilitar esta tarea, se ha diseñado el programa VELERO, con el que se pretende facilitar los procesos de adaptación y desarrollo personal, académico y profesional del alumnado de nuevo ingreso.

Antigenic properties of a transport-competent influenza HA/HIV Env chimeric protein

International Nuclear Information System (INIS)

Ye Ling; Sun Yuliang; Lin Jianguo; Bu Zhigao; Wu Qingyang; Jiang, Shibo; Steinhauer, David A.; Compans, Richard W.; Yang Chinglai

2006-01-01

The transmembrane subunit (gp41) of the HIV Env glycoprotein contains conserved neutralizing epitopes which are not well-exposed in wild-type HIV Env proteins. To enhance the exposure of these epitopes, a chimeric protein, HA/gp41, in which the gp41 of HIV-1 89.6 envelope protein was fused to the C-terminus of the HA1 subunit of the influenza HA protein, was constructed. Characterization of protein expression showed that the HA/gp41 chimeric proteins were expressed on cell surfaces and formed trimeric oligomers, as found in the HIV Env as well as influenza HA proteins. In addition, the HA/gp41 chimeric protein expressed on the cell surface can also be cleaved into 2 subunits by trypsin treatment, similar to the influenza HA. Moreover, the HA/gp41 chimeric protein was found to maintain a pre-fusion conformation. Interestingly, the HA/gp41 chimeric proteins on cell surfaces exhibited increased reactivity to monoclonal antibodies against the HIV Env gp41 subunit compared with the HIV-1 envelope protein, including the two broadly neutralizing monoclonal antibodies 2F5 and 4E10. Immunization of mice with a DNA vaccine expressing the HA/gp41 chimeric protein induced antibodies against the HIV gp41 protein and these antibodies exhibit neutralizing activity against infection by an HIV SF162 pseudovirus. These results demonstrate that the construction of such chimeric proteins can provide enhanced exposure of conserved epitopes in the HIV Env gp41 and may represent a novel vaccine design strategy for inducing broadly neutralizing antibodies against HIV

Characterization of Three Novel Fatty Acid- and Retinoid-Binding Protein Genes (Ha-far-1, Ha-far-2 and Hf-far-1 from the Cereal Cyst Nematodes Heterodera avenae and H. filipjevi.

Directory of Open Access Journals (Sweden)

Fen Qiao

Full Text Available Heterodera avenae and H. filipjevi are major parasites of wheat, reducing production worldwide. Both are sedentary endoparasitic nematodes, and their development and parasitism depend strongly on nutrients obtained from hosts. Secreted fatty acid- and retinol-binding (FAR proteins are nematode-specific lipid carrier proteins used for nutrient acquisition as well as suppression of plant defenses. In this study, we obtained three novel FAR genes Ha-far-1 (KU877266, Ha-far-2 (KU877267, Hf-far-1 (KU877268. Ha-far-1 and Ha-far-2 were cloned from H. avenae, encoding proteins of 191 and 280 amino acids with molecular masses about 17 and 30 kDa, respectively and sequence identity of 28%. Protein Blast in NCBI revealed that Ha-FAR-1 sequence is 78% similar to the Gp-FAR-1 protein from Globodera pallida, while Ha-FAR-2 is 30% similar to Rs-FAR-1 from Radopholus similis. Only one FAR protein Hf-FAR-1was identified in H. filipjevi; it had 96% sequence identity to Ha-FAR-1. The three proteins are alpha-helix-rich and contain the conserved domain of Gp-FAR-1, but Ha-FAR-2 had a remarkable peptide at the C-terminus which was random-coil-rich. Both Ha-FAR-1 and Hf-FAR-1 had casein kinase II phosphorylation sites, while Ha-FAR-2 had predicted N-glycosylation sites. Phylogenetic analysis showed that the three proteins clustered together, though Ha-FAR-1 and Hf-FAR-1 adjoined each other in a plant-parasitic nematode branch, but Ha-FAR-2 was distinct from the other proteins in the group. Fluorescence-based ligand binding analysis showed the three FAR proteins bound to a fluorescent fatty acid derivative and retinol and with dissociation constants similar to FARs from other species, though Ha-FAR-2 binding ability was weaker than that of the two others. In situ hybridization detected mRNAs of Ha-far-1 and Ha-far-2 in the hypodermis. The qRT-PCR results showed that the Ha-far-1and Ha-far-2 were expressed in all developmental stages; Ha-far-1 expressed 70 times more

Characterization of Three Novel Fatty Acid- and Retinoid-Binding Protein Genes (Ha-far-1, Ha-far-2 and Hf-far-1) from the Cereal Cyst Nematodes Heterodera avenae and H. filipjevi.

Science.gov (United States)

Qiao, Fen; Luo, Lilian; Peng, Huan; Luo, Shujie; Huang, Wenkun; Cui, Jiangkuan; Li, Xin; Kong, Lingan; Jiang, Daohong; Chitwood, David J; Peng, Deliang

2016-01-01

Heterodera avenae and H. filipjevi are major parasites of wheat, reducing production worldwide. Both are sedentary endoparasitic nematodes, and their development and parasitism depend strongly on nutrients obtained from hosts. Secreted fatty acid- and retinol-binding (FAR) proteins are nematode-specific lipid carrier proteins used for nutrient acquisition as well as suppression of plant defenses. In this study, we obtained three novel FAR genes Ha-far-1 (KU877266), Ha-far-2 (KU877267), Hf-far-1 (KU877268). Ha-far-1 and Ha-far-2 were cloned from H. avenae, encoding proteins of 191 and 280 amino acids with molecular masses about 17 and 30 kDa, respectively and sequence identity of 28%. Protein Blast in NCBI revealed that Ha-FAR-1 sequence is 78% similar to the Gp-FAR-1 protein from Globodera pallida, while Ha-FAR-2 is 30% similar to Rs-FAR-1 from Radopholus similis. Only one FAR protein Hf-FAR-1was identified in H. filipjevi; it had 96% sequence identity to Ha-FAR-1. The three proteins are alpha-helix-rich and contain the conserved domain of Gp-FAR-1, but Ha-FAR-2 had a remarkable peptide at the C-terminus which was random-coil-rich. Both Ha-FAR-1 and Hf-FAR-1 had casein kinase II phosphorylation sites, while Ha-FAR-2 had predicted N-glycosylation sites. Phylogenetic analysis showed that the three proteins clustered together, though Ha-FAR-1 and Hf-FAR-1 adjoined each other in a plant-parasitic nematode branch, but Ha-FAR-2 was distinct from the other proteins in the group. Fluorescence-based ligand binding analysis showed the three FAR proteins bound to a fluorescent fatty acid derivative and retinol and with dissociation constants similar to FARs from other species, though Ha-FAR-2 binding ability was weaker than that of the two others. In situ hybridization detected mRNAs of Ha-far-1 and Ha-far-2 in the hypodermis. The qRT-PCR results showed that the Ha-far-1and Ha-far-2 were expressed in all developmental stages; Ha-far-1 expressed 70 times more than Ha-far-2 in

Turismo y poder. Las transformaciones de una fiesta popular en Marruecos

Directory of Open Access Journals (Sweden)

María Jesús Berlanga Adell

2004-01-01

Full Text Available En el Alto Atlas Oriental de Marruecos se celebra cada año una fiesta popular conocida comúnmente como el Moussem de Imilchil. La palabra moussem hace referencia a un peregrinaje colectivo que se practica periódicamente en determinada época del año alrededor de la tumba de un santo. El que se desarrolla en la localidad de Imilchil ha sufrido toda una serie de transformaciones a lo largo de los años, cambios que tienen que ver tanto con las relaciones de poder que en esa región bereber se han sucedido, como con el incremento del flujo turístico que ha llegado hacia la zona. Son esas conexiones las que tratamos de analizar en este artículo.

El problema de la integración económica en el campo societario: la experiencia europea.

Directory of Open Access Journals (Sweden)

Camilo Enrique Cubillos Garzón

2006-06-01

Full Text Available En esta primera entrega el autor presenta algunas posturas sobre la conveniencia, y también las dificultades, que genera el hecho de iniciar un proceso de integración económica, partiendo de las experiencias de otros países e incluso el nuestro. De igual forma señala como ha sido esta evolución y hasta que punto se ha llegado en los procesos de integración, para, finalmente, adentrarse en las dificultades que se presentan hoy en día en uno de los procesos de integración económica mejor elaborados que existen hasta el momento, como resulta ser el de la Unión Europea, esto último desde la orbita del Derecho de Sociedades.

El problema de la integración económica en el campo societario: la experiencia europea.

Directory of Open Access Journals (Sweden)

Camilo Enrique Cubillos Garzón

2006-01-01

Full Text Available En esta primera entrega el autor presenta algunas posturas sobre la conveniencia, y también las dificultades, que genera el hecho de iniciar un proceso de integración económica, partiendo de las experiencias de otros países e incluso el nuestro. De igual forma señala como ha sido esta evolución y hasta que punto se ha llegado en los procesos de integración, para, finalmente, adentrarse en las dificultades que se presentan hoy en día en uno de los procesos de integración económica mejor elaborados que existen hasta el momento, como resulta ser el de la Unión Europea, esto último desde la orbita del Derecho de Sociedades.

Una nueva tabla mallorquina de Rafel Mòger

Directory of Open Access Journals (Sweden)

Llompart, P. Gabriel

2001-06-01

Full Text Available El taller pictórico de Rafel Moger fue con mucho, a juzgar por la tablajería que se ha conservado, el más notable que existió en la ciudad de Palma de Mallorca en el tercer cuarto del siglo XV. Lentamente se ha llegado a constituir el catálogo de sus pinturas religiosas y un relativo dossier documental de las religiosas y profanas, realizadas, perdidas y recuperadas a partir del tajo que abrieron los eruditos socios de la Arqueología Luliana, que se pusieron a trabajar a fines del siglo pasado, y, sobre todo, del maestro Chandler Rathfon Post que comenzó a recoger los frutos en su Historia de la Pintura hacia el año 1930.…

Simón Rodríguez Laso, un clérigo ilustrado en el colegio de San Clemente de los españoles

Directory of Open Access Journals (Sweden)

Nieto Sánchez, Carlos

2013-06-01

Full Text Available Simón Rodríguez Laso (Montejo, Salamanca, 1751, Bologna, 1821 Doctor of Law for the University of Bologna, Dignity of the Maestrecuela of the Cathedral of Ciudad Rodrigo, Knight of the Order of Carlos III and Rector of the College of San Clemente (Bolonga, Italy from 1788 until his death, in 1821. This article seeks to highlight the Italian stage of a Spanish Enlightenment men, who fought for the survival of a characteristic institution of the Ancien Regime, San Clemente of the Spaniards College, which resisted the offensive of liberalism. The article also underlines Laso´s personality, learned and regalist, he saw off his reformist thinking because of the new ideas that emerged from French Revolution and devoted his life to the Hispanic College of Bologna.Simón Rodríguez Laso (Montejo, Salamanca, 1751, Bolonia, 1821 Doctor en Cánones por la Universidad de Bolonia, dignidad de de maestrescuela de la S. I. catedral de Ciudad Rodrigo, caballero de la Orden de Carlos III y rector del Colegio de San Clemente de Bolonia desde 1788 hasta su muerte en 1821. En este artículo se pretende dar a conocer la etapa italiana de un ilustrado español, que luchó por la supervivencia de una institución característica del Antiguo Régimen, el Colegio de San Clemente de los españoles de Bolonia, que resistió a la ofensiva del liberalismo. En el artículo se quieren también destacar algunos rasgos de la personalidad de Laso, ilustrado y regalista, que vio mitigado su pensamiento reformista por las nuevas ideas que surgieron de la Revolución Francesa y que consagró su vida al Colegio hispánico de Bolonia.

Fabrication and evaluation of porous Ti–HA bio-nanomaterial by leaching process

Directory of Open Access Journals (Sweden)

A.M. Omran

2015-05-01

Full Text Available A porous surface of Ti–HA composite was successfully fabricated by pulsed current activated sintering (PCAS, followed by leaching using diluted H3PO4. The Ti and HA powders were mixed at different contents of the HA, Ti-5, 10, 30 and 40 wt% HA powders. The mixed powders were pressed in a coated graphite die using pulsed current activated sintering (PCAS under pressure of 60 MPa at temperature of 1000 °C for 5 min. The sintered Ti–HA specimens were immersed in the eight kinds of leaching solutions at room temperature for 24 h. The leached specimen’s surfaces were characterized using XRD, SEM, EDX and Rockwell hardness. The XRD patterns after sintering show that many phases were detected at the sintered specimen surfaces such as; Ti2O, CaO, CaTiO3, TixPy in addition to the remaining Ti and HA. Furthermore, the high concentration H3PO4 leaching solution is more efficient than the low concentration. Also the produced porous surfaces of Ti–HA materials containing more than 30% HA have a low relative density and hardness than the commercial Ti–6Al–4V ELI alloy. In a word, the presence of porous surface coated by HA will promote the nucleation of the biological apatite created with the human tissue and increase the bonding between them. So, the produced porous materials are considered so easy for the muscle cells to permeate after transplanted with high coherence.

CILogon-HA. Higher Assurance Federated Identities for DOE Science

Energy Technology Data Exchange (ETDEWEB)

Basney, James [Univ. of Illinois, Urbana-Champaign, IL (United States)

2015-08-01

The CILogon-HA project extended the existing open source CILogon service (initially developed with funding from the National Science Foundation) to provide credentials at multiple levels of assurance to users of DOE facilities for collaborative science. CILogon translates mechanism and policy across higher education and grid trust federations, bridging from the InCommon identity federation (which federates university and DOE lab identities) to the Interoperable Global Trust Federation (which defines standards across the Worldwide LHC Computing Grid, the Open Science Grid, and other cyberinfrastructure). The CILogon-HA project expanded the CILogon service to support over 160 identity providers (including 6 DOE facilities) and 3 internationally accredited certification authorities. To provide continuity of operations upon the end of the CILogon-HA project period, project staff transitioned the CILogon service to operation by XSEDE.

Wear characterization of nano-hydroxyapatite with addition of titanium (HA-Ti)

Science.gov (United States)

Rosmamuhamadani, R.; Arawi, A. Z. O.; Talari, M. K.; Mahat, M. M.; Bonnia, N. N.; Sabrina; Yahaya, M.; Sulaiman, S.; Ismail, M. I. S.

2018-04-01

Hydroxyapatite (Ca10 (PO4)6(OH)2, HA), is an attractive material of an inorganic compound whose chemical composition and crystallographic structures are similar to the composition of the bone. A natural source such as egg shells is composed of 94 wt. % of calcium carbonate (CaCO3), which can be calcined as calcium oxide (CaO) by the calcinations process. The efficient temperature to produce CaO is 900 °C for 2 hours. The synthesis of nano-HA was done by the mixing the diammonium phosphate (DAP) and calcium hydroxide (Ca(OH)2) and subjected into a microwave for 30 minutes at 1100 W irradiation power. Ball milling process was used for 30 minutes to mix the nano-HA with different compositions of titanium. These were pressed to form pallets by hand hydraulic pump (force=2300 psi). The pallets then were sintered at 1200 °C with the heating rate of 3 °C/min for 2 hours. The pallets were tested by several mechanical testing including hardness, compression strength and wear. From the results, HA-25wt. %Ti composite gave the highest hardness, compression and coefficient of friction for wear test values which were 89.6 Hv, 82.5MPa and 0.76μ respectively. It showed that by adding Ti to nano-HA, the mechanical properties of nano-HA could be enhanced. The microstructure analyses by optical micrograph showed that nano-HA-Ti particles displayed shape likes needle morphology. The particles showed the high tendency to form the agglomerations.

Degradation behavior and compatibility of micro, nanoHA/chitosan scaffolds with interconnected spherical macropores.

Science.gov (United States)

Ruixin, Li; Cheng, Xu; Yingjie, Liu; Hao, Li; Caihong, Shi; Weihua, Su; Weining, An; Yinghai, Yuan; Xiaoli, Qin; Yunqiang, Xu; Xizheng, Zhang; Hui, Li

2017-10-01

Hydroxyapatite/Chitosan (HA/CS) composite have significant application in biomedical especially for bone replacement. Inorganic particle shape and size of composite affect the scaffold mechanical property, biological property, and degradation. The aim of this study was to fabricate HA/CS scaffold with good pore connectivity and analyze their biological, degradation properties. Microhydroxyapatite/chitosan (mHA/CS) and nanohydroxyapatite/chitosan (nHA/CS) composite scaffolds with interconnected spherical pore architectures were fabricated. Composite scaffolds structure parameters were analyzed using micro CT. Cell proliferation and morphology were tested and compared between two scaffolds using mouse osteoblastic cell line MC3T3-E1. To research the composite degradation in lysozyme PBS solution, degradation rate and reducing sugar content were tested, and scaffolds morphology were observed by SEM. The results showed that microHA and nanoHA were fabricated by being calcined and synthesis methods, and their infrared spectra are very similar. EDAX composition analysis demonstrated that both of microHA and nanoHA were calcium deficiency HA. Micro-CT results demonstrated the scaffolds had interconnected spherical pores, and the structure parameters were similar. Cell viabilities were significant increased with cultured time, but there were no significant difference between microHA/CS and nanoHA/CS scaffolds. Scaffold structure was gradually destroyed and inorganic composition HA particles are more prominent with degradation time. (1) Inorganic particle shape and size of composite affect the scaffold mechanical property, biological property, and degradation. NanoHA/CS and microHA/CS scaffolds with good pore connectivity were fabricated and their biological, degradation properties were studied in this manuscript. (2) The scaffold with interconnected porosity construct provides the necessary support for cells to proliferate and maintain their differentiated function, and

Microstructure, mechanical properties, and biological response to functionally graded HA coatings

International Nuclear Information System (INIS)

Rabiei, Afsaneh; Blalock, Travis; Thomas, Brent; Cuomo, Jerry; Yang, Y.; Ong, Joo

2007-01-01

Hydroxyapatite (HA) [Ca 10 (PO 4 ) 6 (OH) 2 ] is the primary mineral content, representing 43% by weight, of bone. Applying a thin layer of HA, to the surface of a metal implant, can promote osseointegration and increase the mechanical stability of the implant. In this study, a biocompatible coating comprising an HA film with functionally graded crystallinity is being deposited on a heated substrate in an Ion Beam Assisted Deposition (IBAD) system. The microstructure of the film was studied using Transmission Electron Microscopy techniques. Finally, initial cell adhesion and cell differentiation on the coating was evaluated using ATCC CRL 1486 human embryonic palatal mesenchymal cell, an osteoblast precursor cell line. The results have shown superior mechanical properties and biological response to the functionally graded HA film

La organización de eventos como estrategia identitaria y evocadora de imagen turística. Estudio de caso: Girona Temps de Flors

Directory of Open Access Journals (Sweden)

Mònika Jiménez Morales

2009-01-01

Full Text Available Desde hace 53 años la ciudad de Girona organiza un evento multitudinario basado en el arte floral que, en sus últimas ediciones, ha llegado a movilizar a más de medio millón de visitantes, muchos de ellos llegados de todas partes de Europa. Más allá de las flores, las exposiciones fotográficas o los montajes audiovisuales que llenan las calles del casco antiguo del municipio durante la segunda semana del mes de mayo, lo cierto es que Girona Temps de Flors representa la puesta en escena de una estrategia comunicativa emergente en el ámbito de la promoción del territorio. En efecto, el evento desempeña, per se, variadas funciones en el ámbito emocional del espacio vivido, pero hay más. También puede devenir a modo de catalizador de imágenes, de creador de imaginarios capaces de fijar una marca de ciudad en su conjunto. El presente artículo dará a conocer las posibilidades que se desprenden de la organización de un evento en relación al posicionamiento identitario y de marca de un territorio.

Intra-articular Hyaluronic Acid (HA) and Platelet Rich Plasma (PRP) injection versus Hyaluronic acid (HA) injection alone in Patients with Grade III and IV Knee Osteoarthritis (OA): A Retrospective Study on Functional Outcome.

Science.gov (United States)

Saturveithan, C; Premganesh, G; Fakhrizzaki, S; Mahathir, M; Karuna, K; Rauf, K; William, H; Akmal, H; Sivapathasundaram, N; Jaspreet, K

2016-07-01

Introduction: Intra-articular hyaluronic acid (HA) is widely utilized in the treatment of knee osteoarthritis whereas platelet rich plasma (PRP) enhances the regeneration of articular cartilage. This study analyses the efficacy of HA and PRP in grade III and IV knee osteoarthritis. Methodology: This is a cross sectional study with retrospective review of 64 patients (101 knees) which includes 56 knees injected with HA+ PRP, and 45 knees with HA only. Results: During the post six months International Knee Documentation Committee (IKDC) evaluation, HA+PRP group showed marked improvement of 24.33 compared to 12.15 in HA group. Decrement in visual analogue score (VAS) in HA+PRP was 1.9 compared to 0.8 in HA group. Conclusion: We propose intra-articular HA and PRP injections as an optional treatment modality in Grade III and IV knee osteoarthritis in terms of functional outcome and pain control for up to six months when arthroplasty is not an option.

Intra-articular Hyaluronic Acid (HA and Platelet Rich Plasma (PRP injection versus Hyaluronic acid (HA injection alone in Patients with Grade III and IV Knee Osteoarthritis (OA: A Retrospective Study on Functional Outcome

Directory of Open Access Journals (Sweden)

Saturveithan C

2016-07-01

Full Text Available Introduction: Intra-articular hyaluronic acid (HA is widely utilized in the treatment of knee osteoarthritis whereas platelet rich plasma (PRP enhances the regeneration of articular cartilage. This study analyses the efficacy of HA and PRP in grade III and IV knee osteoarthritis. Methodology: This is a cross sectional study with retrospective review of 64 patients (101 knees which includes 56 knees injected with HA+ PRP, and 45 knees with HA only. Results: During the post six months International Knee Documentation Committee (IKDC evaluation, HA+PRP group showed marked improvement of 24.33 compared to 12.15 in HA group. Decrement in visual analogue score (VAS in HA+PRP was 1.9 compared to 0.8 in HA group. Conclusion: We propose intra-articular HA and PRP injections as an optional treatment modality in Grade III and IV knee osteoarthritis in terms of functional outcome and pain control for up to six months when arthroplasty is not an option.

A-Ha. Drum'n'bassi supernimi FABIO

Index Scriptorium Estoniae

2002-01-01

7.sept. annab norra menukaim popansambel A-Ha Tallinnas Lauluväljakul kontserdi, kus presenteerib ka oma viimast albumit "Lifelines". 14. sept.tuleb drum'n'bassi spetsialist Fabio Tallinna üritusele Circulation

Mechanical strength of [HA/Bioplastic/Sericin] composite part printed by bioprinter

Energy Technology Data Exchange (ETDEWEB)

Tontowi, Alva Edy, E-mail: alvaedytontowi@ugm.ac.id; Setiawan, Agris [Department of Mechanical and Industrial Engineering Faculty of Engineering Universitas Gadjah Mada (Indonesia)

2016-06-17

The aim of this research was to determine the effect of hydroxyapatite (HA) content in printed biocomposite to its mechanical strength. The biocomposite paste was prepared by composing HA, bioplastic and sericin with various ratios of [HA/Bioplastic]: 40/60, 50/50, 60,40 and 70/30. Sericin of 0.3% weight was added to the biocomposite. Mechanical test was conducted to observe tensile (ASTM D 638 type 4) and flexural strength (ASTM D 790). Both type of specimens were fabricated using 3D Printer. Printing process parameter (infill speed, print speed and layer height) were set up as 60 mm/s, 10 mm/s, 0.35 mm, respectively. Results showed that biocomposite with [HA/Biplastic]. weight ratio of 60/40(w/w) has an optimum tensile (3.89 ± 1.26 MPa) and flexural strength (2.51 ± 0.45 MPa). Scanning electron microscope observation indicated that microstructure of specimen was influenced by the percentage of the hydroxyapatite. There was no agglomeration of HA particle within the composite.

Mechanical strength of [HA/Bioplastic/Sericin] composite part printed by bioprinter

International Nuclear Information System (INIS)

Tontowi, Alva Edy; Setiawan, Agris

2016-01-01

The aim of this research was to determine the effect of hydroxyapatite (HA) content in printed biocomposite to its mechanical strength. The biocomposite paste was prepared by composing HA, bioplastic and sericin with various ratios of [HA/Bioplastic]: 40/60, 50/50, 60,40 and 70/30. Sericin of 0.3% weight was added to the biocomposite. Mechanical test was conducted to observe tensile (ASTM D 638 type 4) and flexural strength (ASTM D 790). Both type of specimens were fabricated using 3D Printer. Printing process parameter (infill speed, print speed and layer height) were set up as 60 mm/s, 10 mm/s, 0.35 mm, respectively. Results showed that biocomposite with [HA/Biplastic]. weight ratio of 60/40(w/w) has an optimum tensile (3.89 ± 1.26 MPa) and flexural strength (2.51 ± 0.45 MPa). Scanning electron microscope observation indicated that microstructure of specimen was influenced by the percentage of the hydroxyapatite. There was no agglomeration of HA particle within the composite.

New Coll–HA/BT composite materials for hard tissue engineering

Energy Technology Data Exchange (ETDEWEB)

Zanfir, Andrei Vlad [Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, “Politehnica” University of Bucharest, 1-7 Gh. Polizu Street, RO-011061 Bucharest (Romania); Voicu, Georgeta, E-mail: getav2001@yahoo.co.uk [Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, “Politehnica” University of Bucharest, 1-7 Gh. Polizu Street, RO-011061 Bucharest (Romania); Busuioc, Cristina; Jinga, Sorin Ion [Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, “Politehnica” University of Bucharest, 1-7 Gh. Polizu Street, RO-011061 Bucharest (Romania); Albu, Madalina Georgiana [Department of Collagen, Branch of Leather and Footwear Research, National Institute of Research and Development for Textile and Leather, 93 I. Minulescu Street, RO-031215 Bucharest (Romania); Iordache, Florin [Department of Fetal and Adult Stem Cell Therapy, “Nicolae Simionescu” Institute of Cellular Biology and Pathology of Romanian Academy, 8 B.P. Hasdeu Street, RO-050568 Bucharest (Romania)

2016-05-01

The integration of ceramic powders in composite materials for bone scaffolds can improve the osseointegration process. This work was aimed to the synthesis and characterization of new collagen–hydroxyapatite/barium titanate (Coll–HA/BT) composite materials starting from barium titanate (BT) nanopowder, hydroxyapatite (HA) nanopowder and collagen (Coll) gel. BT nanopowder was produced by combining two wet-chemical approaches, sol–gel and hydrothermal methods. The resulting materials were characterized in terms of phase composition and microstructure by X-ray diffraction, Raman spectroscopy, scanning electron microscopy and transmission electron microscopy. Moreover, the biocompatibility and bioactivity of the composite materials were assessed by in vitro tests. The synthesized BT particles exhibit an average size of around 35 nm and a spherical morphology, with a pseudo-cubic or tetragonal symmetry. The diffraction spectra of Coll–HA and Coll–HA/BT composite materials indicate a pronounced interaction between Col and the mineral phases, meaning a good mineralization of Col fibres. As well, the in vitro tests highlight excellent osteoinductive properties for all biological samples, especially for Coll–HA/BT composite materials, fact that can be attributed to the ferromagnetic properties of BT. - Highlights: • Collagen–hydroxyapatite/barium titanate composite materials were synthesized. • Barium titanate was produced by combining the sol–gel and hydrothermal methods. • The in vitro tests highlight excellent osteoinductive properties for all samples.

New Coll–HA/BT composite materials for hard tissue engineering

International Nuclear Information System (INIS)

Zanfir, Andrei Vlad; Voicu, Georgeta; Busuioc, Cristina; Jinga, Sorin Ion; Albu, Madalina Georgiana; Iordache, Florin

2016-01-01

The integration of ceramic powders in composite materials for bone scaffolds can improve the osseointegration process. This work was aimed to the synthesis and characterization of new collagen–hydroxyapatite/barium titanate (Coll–HA/BT) composite materials starting from barium titanate (BT) nanopowder, hydroxyapatite (HA) nanopowder and collagen (Coll) gel. BT nanopowder was produced by combining two wet-chemical approaches, sol–gel and hydrothermal methods. The resulting materials were characterized in terms of phase composition and microstructure by X-ray diffraction, Raman spectroscopy, scanning electron microscopy and transmission electron microscopy. Moreover, the biocompatibility and bioactivity of the composite materials were assessed by in vitro tests. The synthesized BT particles exhibit an average size of around 35 nm and a spherical morphology, with a pseudo-cubic or tetragonal symmetry. The diffraction spectra of Coll–HA and Coll–HA/BT composite materials indicate a pronounced interaction between Col and the mineral phases, meaning a good mineralization of Col fibres. As well, the in vitro tests highlight excellent osteoinductive properties for all biological samples, especially for Coll–HA/BT composite materials, fact that can be attributed to the ferromagnetic properties of BT. - Highlights: • Collagen–hydroxyapatite/barium titanate composite materials were synthesized. • Barium titanate was produced by combining the sol–gel and hydrothermal methods. • The in vitro tests highlight excellent osteoinductive properties for all samples.

Teoría del desplazamiento. Ensayo sobre los discursos hegemónicos en el sistema artístico español

Directory of Open Access Journals (Sweden)

Tomás Ruiz-Rivas

2014-06-01

Full Text Available Este año, 2006, la feria de arte contemporáneo de Madrid ARCO celebró su 25o aniversario con un baño de triunfalismo y autosatisfacción. La que fue su directora durante dos décadas, Rosina Gómez Baeza, ofreció un balance en el que la enumeración de logros era interminable, y acabó por afirmar que ARCO ha llegado a ser el centro de gravedad del arte español. ARCO, según hemos oído insistentemente en ruedas de prensa, declaraciones y actos sociales, ha pasado de las 90 galerías de su primera edición en 1982, a casi 300 en las últimas ediciones y de 25.000 visitantes a 200.000, ha promovido el coleccionismo, el mecenazgo y diversas (y suponemos que acertadas políticas culturales. También ha democratizado el arte más vanguardista, ha impulsado la creación, ha canalizado el debate teórico, etc. Este éxito arrollador choca con la penuria de nuestra creación artística: la presencia de artistas españoles en los circuitos internacionales es inapreciable, y es excepcional que se les invite a las principales exposiciones.

Concepto lego de estrés en estudiantes universitarios

OpenAIRE

Revuelta Pérez, Francisco; Rodríguez Fernández, Luisa María; Atienza Martín, Francisco Javier; Losada Ruiz, Carmen

2013-01-01

El término estrés ha llegado a ser uno de los más utilizados en el contexto de la salud y la enfermedad. No obstante, no hay un consenso generalizado acerca de su definición por parte de los profesionales pertinentes, como tampoco por lo que respecta a los no expertos. Si es importante esta cuestión en relación con los primeros, no deja de serlo para los segundos. En general, las creencias, conceptos y teorías legas afectan a las percepciones de las personas y a las ...

"Destinado de papel de impresión láser por medio magnético-mecánico".

OpenAIRE

Ramírez Valdovinos, Enrique

2011-01-01

El equilibrio ecológico y ambiental ha llegado a puntos críticos, causando consecuencias graves e irreversibles a nuestro entorno natural, debido al desarrollo industrial por parte de las grandes potencias mundiales, países en vías de desarrollo e incluso países del tercer mundo. La presente investigación, propone el uso de un nuevo equipo que genera fuerzas magnéticas-mecánicas para el destintado de la pulpa para la fabricación de papel, evitando con este equipo el uso de agentes químicos. E...

El periodismo del tercer milenio. problemas de la divulgación científica en iberoamérica

OpenAIRE

Calvo Hernando, Manuel

2002-01-01

En nuestro tiempo han surgido unos profesionales de la información cuya misión es explicar al público el universo y hacer partícipe a la mayoría de los descubrimientos e investigación de esa minoría integrada por los hombres de ciencia. El periodismo científico constituye un instrumento para la democracia, porque facilita a gran número de personas poder seguir los avances del conocimiento. Por el momento, esta idea no ha llegado plenamente al público y ni siquiera a los políticos ni a los res...

Enfoques alrededor de la gestión estratégica de la innovación

OpenAIRE

Universidad del Rosario, Facultad de Administración. Centro de Estudios Empresariales para la Perdurabilidad - CEEP. Grupo de Investigación en Perdurabilidad Empresarial. Línea de Investigación en Nuevas Tecnologías; Pineda Serna, Leonardo

2009-01-01

La innovación ha llegado a convertirse en el tema de mayor trascendencia en el contexto global. Este documento de trabajo trata de sintetizar los principales aspectos asociados a esta temática. Partiendo de una definición genérica se relaciona el término de innovación con los diferentes niveles que se asocian a su análisis sistémico, para de allí definir el significado de la novedad y aplicación que debe llevar toda innovación. El éxito de la innovación solo lo valida el mer...

El Quijote en Europa y América en los siglos XVIII y XIX

OpenAIRE

Álvarez Barrientos, Joaquín

2005-01-01

[ES] El primer título que aparece en pantalla en Fahrenheit 451, la película de François Truffaut de 1966, tras llegar los bomberos a la casa denunciada para quemar los libros, es Don Quijote de la Mancha, traducido al inglés, que está oculto en una lámpara. Que sea el primero no es casual: da cuenta de hasta qué punto esa obra ha llegado a ser patrimonio de todos, un hito reconocible en cualquier lugar del mundo. Pero desde luego, dado el asunto que trata la novela de Ray Bradbur...

La ganadería ecológica como modelo de gestión del Parque Natural de la Sierra de Grazalema

OpenAIRE

Díaz Gaona, Cipriano

2013-01-01

1.introducción o motivación de la tesis La mayoría de los Espacios Naturales Protegidos (ENP) de España han llegado a serlo gracias al uso racional que de sus recursos han venido haciendo sus pobladores durante siglos; especialmente, a través de la ganadería. Esta ganadería sostenible ha ido perdiendo rentabilidad en los últimos años, lo que está llevando a muchos ganaderos a intensificar sus explotaciones o a abandonarlas; lo cual repercute negativamente sobre la conservación de dichos E...

Information and Communication Technology Strategy in the Hospitality Industry

OpenAIRE

Connolly, Daniel; Bustos Ríos, Ligia Stella; Moreno Laverde, Ricardo

2008-01-01

Este documento mira la importancia estratégica cada vez mayor de las tecnologías de información y de las comunicaciones (TIC) en la industria de la hospitalidad. En las últimas cuatro décadas, la industria de la hospitalidad ha experimentado cambios dramáticos como resultado de innovaciones y de progresos de la tecnología. Uno podría discutir fácilmente que el negocio haya llegado a ser mucho más complejo, sofisticado e intensivo en costos con la evolución y la adopción de la tecnología a t...

Estudio exploratorio sobre identificación de seres vivos autóctonos en las imágenes de libros de texto de Ciencias de la Naturaleza y en alumnos de Enseñanza Secundaria Obligatoria

OpenAIRE

Marqués-Perales, Raúl

2013-01-01

Esta investigación tiene por objeto medir la presencia de seres vivos autóctonos de España en los libros de texto de Enseñanza Secundaria Obligatoria y el grado de conocimiento que poseen los estudiantes de Secundaria acerca de éstos. Por un lado, se han encuestado a 76 estudiantes de Secundaria mediante cuestionario con imágenes de seres vivos de la zona donde se realizó el cuestionario. La principal conclusión a la que se ha llegado tras el análisis de los resultados obtenidos es que la ...

Pasado, presente y futuro cercano de los materiales para uso en implantes óseos biodegradables

OpenAIRE

Plazas Bonilla, Clara Eugenia; Perilla, Jairo Ernesto

2011-01-01

El manejo de fracturas y defectos óseos procura la regeneración del tejido óseo respetando su anatomía y buscando recuperar su funcionalidad. El logro de este objetivo se inició en el siglo XVII con el uso de injertos de origen animal o humano, y ha llegado en la actualidad al uso de implantes, dispositivos elaborados en materiales naturales o sintéticos que comportan una gran variedad de propiedades químicas, físicas y biológicas. Las investigaciones en el área continúan encaminándose a la s...

Desarrollo de tecnologías de baja temperatura para la fabricación de células solares de heterounión de silicio

OpenAIRE

Morales Vilches, Anna Belén

2015-01-01

El desarrollo de la tecnología fotovoltaica de silicio cristalino ha llegado al punto en que la mejora de los dispositivos pasa, no tanto por un aumento de la eficiencia, como por la reducción del coste de fabricación. En este tipo de dispositivos el coste más importante se debe al propio sustrato de silicio cristalino. De manera que una reducción en el grosor de la oblea se reflejaría directamente en costes de producción más bajos, al disminuir la cantidad de material utilizado como sustrato...

Internet e historia contemporánea de España

Directory of Open Access Journals (Sweden)

Ángel Martínez de Velasco Farinós

1995-01-01

Full Text Available Internet es un término de origen informático del que poco se sabe aunque mucho se habla; tanto que ha llegado a ser un auténtico mito sociocultural. En este artículo se intenta explicar la joven historia de Internet como medio para llegar a la descripción de los servicios que pueden ser útiles para los historiadores. Posteriormente se muestra la aplicación de Internet hecha para el Departamento de Historia Contemporánea de la UNED y finaliza con un breve comentario bibliográfico.

Edición crítica, estudio y notas de una comedia de Tirso de Molina: la elección por la virtud

OpenAIRE

Galindo Abellán, Miguel

2012-01-01

Desde su publicación en la Tercera Parte de comedias del Maestro Tirso de Molina, impresa en Tortosa (1634), La elección por la virtud ha llegado a nosotros a través de seis ediciones posteriores, pero ninguna de ellas con edición crítica. En esta tesis se catalogan errores y erratas que han ido transmitiéndose de texto en texto, se ofrece un estudio pormenorizado de la comedia, se estudia su métrica y se anotan aspectos lingüísticos y culturales de cada escena, además de estudiar la comedia ...

DUST FORMATION BY COLLIDING-WIND BINARIES

OpenAIRE

P. M. Williams

2008-01-01

Imágenes infrarrojas de alta resolución recientes de las enigmáticas estrellas cocoon en el cúmulo del Quintuplete, muestran comas tipo "pinwheel". La inferencia de que las estrellas son binarias Wolf-Rayet con vientos en colisión evidencia cuán lejos ha llegado el estudio de la formación de polvo en los vientos, desde el primer descubrimiento de polvo de carbono calentado, alrededor de la clásica estrella WC9 Ve2-45 (WR 104). La formación de polvo atribuida a vientos en colisión es el proces...

Prótesis total y unicompartimental en el manejo de artrosis grave de rodilla

OpenAIRE

Jaime duboy, U.

2014-01-01

La artrosis grave de rodilla es una patología prevalente y puede ser en algunos casos invalidante. Se revisan los fundamentos e historia sobre cómo la prótesis de rodilla ha llegado a ser el tratamiento de elección para eliminar el dolor y recuperar función, mejorando la calidad de vida de los pacientes. Se muestran las alternativas actuales que incluyen las prótesis Unicompartamentales, de creciente uso en los últimos años y se señalan también las mejoras en los cuidados perioperatorios y re...

La situación de la ética en las empresas del IBEX 35: un análisis de la misión, visión, valores corporativos y códigos éticos

OpenAIRE

Llácer Centeno, Pilar

2016-01-01

El objetivo de esta tesis es la reflexión sobre el estado de la ética en las empresas del IBEX 35. Analizando su misión, visión, valores corporativos y códigos éticos para demostrar que no se ha llegado a un nivel de implantación suficiente, ni a una coherencia en las pautas de comportamiento ético con los diferentes stakeholders. Mi análisis pretende integrar el enfoque tradicional de la ética de los negocios con la ética de las personas, incorporando los entornos tecnológicos como espacios ...

consideración de los alumnos de educación física

Directory of Open Access Journals (Sweden)

Pedro Gil Madrona

2007-01-01

Full Text Available Tras el acuerdo de Bolonia han llegado nuevos tiempos en la Unión Europea para la Educación Superior. La innovación y, más aún, la investigación educativa no puede quedar al margen de cualquier cambio, máxime de los cambios que se avecinan, principalmente cuando dichos cambios afectarán a la forma de enseñar de los profesores y al modo de aprendizaje o de enfrentarse a las enseñanzas propuestas por los denominados créditos ECTS por parte de los alumnos. Es por ello por lo que a unos, los profesores, a otros, los alumnos, y a otros terceros, los responsables de las instituciones superiores (diseñadores de los planes de estudios, directivos y administradores de instalaciones docentes precisan estar al tanto de cuáles son los estilos de aprendizaje de las personas que acuden a la universidad con la intención de hacer una determinada carrera. En este sentido, el caso que nos ocupa en esta investigación es conocer el modo de aprendizaje, los estilos de aprendizaje de los estudiantes de Magisterio en la Universidad de Castilla-La Mancha en las diferentes especialidades. Saber al dedillo las semejanzas y diferencias entre unos y otros estudiantes. La finalidad no es otra que dar luz a la comunidad universitaria (alumnos, profesores y decanos con estos informes sobre los estilos de aprendizaje del alumnado, para que cada cual en su respectiva parcela de actuación tome las medidas adecuadas.

Human hypervariable sequences in risk assessment: rare Ha-ras alleles in cancer patients

International Nuclear Information System (INIS)

Krontiris, T.G.; DiMartino, N.A.; Mitcheson, H.D.; Lonergan, J.A.; Begg, C.; Parkinson, D.R.

1987-01-01

A variable tandem repeat (VTR) is responsible for the hyperallelism one kilobase 3' to the human c-Ha-ras-1 (Ha-ras) gene. Thirty-two distinct restriction fragments, comprising 3 allelic classes by frequency of occurrence, have thus far been detected in a sample size of approximately 800 caucasians. Rare Ha-ras alleles, 21 in all, are almost exclusively confined to the genomes of cancer patients. From their data the authors have computed the relative cancer risk associated with possession of a rare Ha-ras allele to be 27. To understand the molecular basis for this phenomenon, they have begun to clone Ha-ras fragments from nontumor DNA of cancer patients. They report here the weak activation, as detected by transfection and transformation of NIH 3T3 mouse cells, of two Ha-ras genes which were obtained from lymphocyte DNA of a melanoma patient. They have mapped the regions that confer this transforming activity to the fragment containing the VTR in one Ha-ras clone and the fragment containing gene coding sequences in the other

Poredbena analiza Haških i Roterdamskih pravila

Directory of Open Access Journals (Sweden)

Vesna Skorupan Wolff

2010-12-01

Full Text Available Primjenom komparativne metode uspoređuju se najvažnije konvencijske odredbe i rješenja Roterdamskih pravila s onima sadržanim u Haškim pravilima. Raščlanjuje se većina recentnih odredbi Roterdamskih pravila koje predstavljaju ključne elemente po kojime se ona razlikuju od Haških pravila. U okviru toga ukazuje se na važnost novih pojmova, instituta i osoba koje sudjeluju u prijevoznom poslu. Govori se i o važnim odredbama koje su podudarne jer upravo brojna tradicionalna rješenja koja donose Roterdamska pravila mogu pridonijeti njihovom bržem i lakšem akceptiranju. Roterdamska pravila nastoje na suvremeni način urediti ugovore o prijevozu stvari morem, a pod određenim uvjetima primjenjivat će se i na prijevoze drugim prijevoznim granama. Opsežnost konvencije govori o njezinoj sveobuhvatnosti i velikoj preciznosti u propisivanju gotovo svih pitanja iz područja pravnog uređenja pomorskih prijevoznih ugovora, i više od toga, ona je jedinstven primjer temeljite raščlambe svih obveza i odgovornosti ugovornih stranaka i općenito svih subjekata koji se pojavljuju u prijevoznom poslu. Cilj poredbene analize Haških i Roterdamskih pravila je spoznati što novo donose Roterdamska pravila i procijeniti kako se ona uklapaju u postojeće pravno uređenje prijevoza stvari morem. Istodobno cilj je spoznati koje se odredbe Haških pravila danas izlažu kritici zbog njihove neprilagođenosti suvremenim uvjetima prijevoza i novim i drugačijim odnosima snaga između ugovornih strana u pomorskim prijevoznim ugovorima.

New therapeutic agent for radiation synovectomy - preparation of 166Ho-EDTMP-HA particle

International Nuclear Information System (INIS)

Bai, H.; Jin, X.; Du, J.; Wang, F.; Chen, D.; Fan, H.; Cheng, Z.; Zhang, J.

1997-01-01

In order to prepare new therapeutical agent for radiation synovectomy, Hydroxyapatite (HA) was labelled with 166 Ho by EDTMP that had high affinity to HA particles. Radiolabelling of HA particles was divided into two steps, 166 Ho-EDTMP was prepared first; then mixed with HA particles completely and vibrated for 15 minutes on the micromixer at room temperature, washed 3 times with deionized water. Radiolabelling particle was separated from free 166 Ho via centrifugation to determine its radiolabelling efficiency. 166 Ho-EDTMP-HA and 166 Ho-EDTMP were injected into knee joint of normal rabbits respectively, every group was killed at different time postinjection, took out major organ and collected urine and blood, then weighted and determined their radio counts. HA particles, as a natural component of bone was known to have good compatibility with soft tissue and biodegrade into calcium and phosphate in vivo. It was readily prepared from common chemical and formed into particles of desired size range in a controlled process, it had high stability in vitro and vivo. Radiolabelling of HA particle with 166 Ho by EDTMP was simple to perform and provides an excellent labelling yield that was more than 95% under the optimal labelling condition. The optimal labelling condition at room temperature was pH 6.0-8.0 and vibration time 15 minutes. The absorbed capacity of HA particle was 5 mg Ho/g HA particle and size of radiolabelling particle was at range of 2-5,μm that is suitable for therapy of radiation synovectomy. 166 Ho-EDTMP-HA particle demonstrated high in vitro stability in either normal saline or 1% BSA solution, but instability under extremely acidic condition (pH 1-2). The control studies performed with 166 Ho-EDTMP not bound to HA particle provided information on the distribution of radioactivity that would occur upon leakage of the radiochemical compound from joint. Its short half-life, its extremely low leakage from the joint and its even distribution throughout

'HUBO UN TIEMPO NO TAN LEJANO...' RELATOS Y ESTÉTICAS DE LA MEMORIA E IDEOLOGÍA DE LA RECONCILIACIÓN EN ESPAÑA

Directory of Open Access Journals (Sweden)

Jaume Peris Blanes

2014-10-01

Full Text Available En la última década se han consolidado en España nuevos estilos culturales con los que representar el pasado reciente, en estrecha relación con la emergencia de los debates en torno a la llamada memoria histórica y con el desarrollo contemporáneo de las industrias culturales. Esos estilos han llegado a estandarizar una serie de procedimientos de composición y efectos textuales que permiten que el público los identifique con las estéticas de la memoria. El testimonio, como forma paraliteraria y discurso cívico, ha alcanzado un lugar de excepción en esa estandarización de las estéticas de memoria, pero su uso mecanizado ha privilegiado su rentabilidad dramática sobre su potencial crítico.

Hi ha un paper per a l'ètica a l'empresa?

OpenAIRE

Argandoña Rámiz, Antonio

2001-01-01

No hi ha decisions ètiques i decisions no ètiques. Hi ha decisions: l'ètica és, simplement, un component més de cada decisió —i, per tant, molt important. No hi ha directors d'empresa ètics i altres que no ho són: hi ha directors que dirigeixen bé, i directors que dirigeixen malament. Els que dirigeixen bé són ètics. Si no són ètics, estan perdent algun aspecte de la realitat que és necessari per a la seva tasca de dirigir: per tant, dirigeixen malament. Però, a més a més, ser ètic no és tan ...

Development and characterization of poli composites (ether ether ketone)(PEEK)(Hydroxyapatite(HA); Desenvolvimento e caracterizacao de compositos poli (eter-eter-cetona)(PEEK)/Hidroxiapatita(HA)

Energy Technology Data Exchange (ETDEWEB)

Ferreira, V.P.; Santos, F.S.F.; Sa, M.D. de; Fook, M.V.L., E-mail: valeriap.ferreira@hotmail.com [Universidade Federal de Campina Grande (UFCG), PB (Brazil). Centro de Ciencias e Tecnologia. Programa de Pos-Graduacao em Ciencia e Engenharia de Materiais

2016-07-01

The objective of this work was to develop PEEK / HA composites, combining the biological activity of the ceramic phase with the properties of the polymer phase, the materials used in this research were Poly (ether-ether-ketone) (PEEK) and Hydroxyapatite (HA) (50, 60, 70 and 80% m / v HA), this material was subjected to a load of two tons followed by a thermal treatment at 390 ° for a period of 30 minutes. Then they were characterized by FTIR, DRX and MO. In the physical-chemical characterization of FTIR and XRD, it was not possible to identify significant alterations. In the FTIR spectra of the composites, there is no formation of new identifiable chemical bonds. In the composites XRD diffractograms a profile similar to the ceramic phase was observed, with peaks increasing in intensity and narrowing proportional to the increase of the hydroxyapatite concentration in the composites. In optical microscopy it is possible to observe surfaces with heterogeneous morphology, with signs of roughness and in the cross section we observe a heterogeneous aspect, rich in regions with large agglomerates and lighter particles. Considering the processing aspects, the technique proved to be effective for the development of PEEK /HA composites. (author)

Preparation and characterization of NiW-nHA composite catalyst for hydrocracking

Science.gov (United States)

Zhou, Gang; Hou, Yongzhao; Liu, Lei; Liu, Hongru; Liu, Can; Liu, Jing; Qiao, Huiting; Liu, Wenyong; Fan, Yubo; Shen, Shituan; Rong, Long

2012-11-01

The synthesis, characterization and catalytic capability of the NiW-nano-hydroxyapatite (NiW-nHA) composite were investigated in this paper. The NiW-nHA catalyst was prepared by a co-precipitation method. Then Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and energy dispersive spectroscopy (EDX) were used to analyze this material. In addition, the catalytic capacity of the NiW-nHA composite was also examined by FT-IR and gas chromatography (GC). The results of FT-IR analysis indicated that Ni, W and nHA combined closely. TEM observation revealed that this catalyst was needle shaped and the crystal retained a nanometer size. XRD data also suggested that a new phase of CaWO4 appeared and the lattice parameters of nHA changed in this system. nHA was the carrier of metals. The rates of Ni/W-loading were 73.24% and 65.99% according to the EDX data, respectively. Furthermore, the conversion of 91.88% Jatropha oil was achieved at 360 °C and 3 MPa h-1 over NiW-nHA catalyst. The straight chain alkanes ranging from C15 to C18 were the main components in the production. The yield of C15-C18 alkanes was up to 83.56 wt%. The reaction pathway involved hydrocracking of the C&z.dbd;C bonds of these triglycerides from Jatropha oil. This paper developed a novel non-sulfided catalyst to obtain a ``green biofuel'' from vegetable oil.

Investigation of microstructure and mechanical properties of PLLA/HA system

Science.gov (United States)

Nik Hanyn N., H.; Ahmad, K. Z. Ku; Yusof, Mohd Reusmaazran; Kamarolzaman, A. A.

2017-12-01

The poly(L-lactide) (PLLA), carboxymethyl starch (CMS) and hydroxyapatite (HAp) nanofibers have been prepared by electrospinning technique. The PLLA/CMS formulations applied at various voltage were studied for electrospinning into nanofibers. It was found that the morphology of the fibers became finer with the increment of voltage applied. The formulation with 10 kV voltage was selected as it shows promising result based on the morphology observation. The PLLA/CMS/HA formulation with HA loading concentration (i.e. 0.25, 0.50, 0.75 and 1.00 wt%) was fabricated in the next stage, and the effect of HA addition was studied. Observation showed that the electrospun nanofibers' diameters were reduced with the addition of HAp concentration from 0.25 wt% HAp to 1.00 wt% Hap.

Mao & Püha Eliisabet / Jüri Kuuskemaa

Index Scriptorium Estoniae

Kuuskemaa, Jüri, 1942-

2000-01-01

Kadrioru lossis Väliskunstimuuseumi ekspositsioonis olevast antikvaar August Käbi pärandist pärit maalist. Kunstiteadlase Mai Levini arvates võib maali autoriks olla Rooma kunstnik Tommaso Salini, hüüdnimega Mao. Maali süzee: "Püha Eliisabeti kuulutus". 1 ill

Prediction of IOI-HA Scores Using Speech Reception Thresholds and Speech Discrimination Scores in Quiet

DEFF Research Database (Denmark)

Brännström, K Jonas; Lantz, Johannes; Nielsen, Lars Holme

2014-01-01

), and speech discrimination scores (SDSs) in quiet or in noise are common assessments made prior to hearing aid (HA) fittings. It is not known whether SRT and SDS in quiet relate to HA outcome measured with the International Outcome Inventory for Hearing Aids (IOI-HA). PURPOSE: The aim of the present study...... COLLECTION AND ANALYSIS: The psychometric properties were evaluated and compared to previous studies using the IOI-HA. The associations and differences between the outcome scores and a number of descriptive variables (age, gender, fitted monaurally/binaurally with HA, first-time/experienced HA users, years...

El griot no ha muerto, viva el hip-hop

Directory of Open Access Journals (Sweden)

Fco. Javier González García-Mamely

2015-11-01

Full Text Available La oratura en África ha jugado un papel vital a lo largo de los milenios como un (único medio de preservar y transmitir la historia, la cultura y el imaginario de cada comunidad. El griot es un claro modelo de las artes orales, pero ha sufrido cambios drásticos en su modo de vida y en los recursos narrativos de los que dispone. Tras el mecenazgo de los grandes reyes y las sucesivas llegadas del islam, el colonialismo y los procesos de modernización y occidentalización, los griots se han tenido que adaptar a nuevos patrones, audiencias y medios de comunicación, convirtiéndose en políticos o en figuras del espectáculo. La aparición del rap y el hip-hop reclamando ser los “modernos griots” ha ocasionado una respuesta crítica y una atracción viral hacia el nuevo fenómeno, sea éste un nuevo género o una reinvención de la oratura del antiguo griot.

Changes of serum HA and LN level in patients with pulmonary tuberculosis after therapy

International Nuclear Information System (INIS)

Yu Jing Zhang Hongwei; Li Jie

2007-01-01

Objective: To study the serum HA and LN level in patients with pulmonary tuberculosis and their response to therapy. Methods: Serum HA and LN levels were measured with RIA in: (1) 42 patients with active pulmonary tuberculosis both before and after 2 months' therapy, (2) 40 patients with non-active pulmonary TB and 330 controls. Results: The serum HA and LN level in patients with active pulmonary tubemulosis were significantly higher than those in controls (P < 0.01 ), while no significant difference could Be found Between the levels in patients with non-active pulmonary tuberculosis and controls. The serum HA and LN level in patients with active pulmonary tuberculosis dropped after therapy, but were still higher than those in controls. Significant correlation could Be found between HA and LN levels (P<0.01). The HA and LN levels were positively correlated with severity of the disease, ESR and CRP contents. Conclusion: The measurement of serum HA and LN is valuable for early diagnosis, monitoring development and assessment of therapeutic effect in patients with pulmonary tubemulosis. (authors)

166Ho-HA evaluation as therapeutic agent for rheumatoid arthritis treatment

International Nuclear Information System (INIS)

Chandia, M.C.; Errazu, X.C.; Pinto, L.N.; Godoy, N.O.; Avila, M.J.; Mendoza, P.; Mendoza, J.; Jofre, J.; Sirraalta, P.

2002-01-01

Aim: Rheumatoid arthritis is a limiting disease having, among its pathological features, the inflammation of synovial tissue with progressive and later destruction of the articulation. This leads to joint deformation and loss of its function, generating pain and reducing the mobility of the affected articulation. The aim was to evaluate 166 Ho-Hydroxyapatite ( 166 Ho-HA) as potential radiopharmaceutical for the symptomatic treatment of chronic and acute arthritis. Materials and Methods: Holmiun-166 was produced by irradiation of Ho 2 O 3 at La Reina Research Reactor, Nuclear Chilean Energy Commission. Hydroxyapatite was in-house synthesized. Its labelling and quality controls follows the internationally accepted procedures. An antigen's arthritis was induced to eight New Zealand rabbits with the 166 Ho-HA radiochemical being administered thereafter in two dosage modalities (single and double). The compound therapeutic efficiency was evaluated based upon clinical improvement and images from the inflamated articulation using 67 Ga citrate before and after 166 Ho-HA injection. Results: The radiochemical purity of the inoculated compound was greater than 98% as measured under sterility conditions. Clinically, an inflammation reduction (2 cm), appetite improvement and general well being was observed. The 166 Ho-HA distribution and localization was monitored using gamma camera images taken at 4 and 24 h. There was no evidence of extra articular leakage. From the 67 Ga citrate imaging, the acute group shows an overall improvement of well being corresponding to a lesser uptake at the inflamated articulation, regarding to the chronic group. The 166 Ho-HA double doses, compared to the single doses, suggest a reduced uptake of 67 Ga citrate at the inflamated tissue, meaning an increased therapeutic effect. Conclusions: 166 Ho-HA is useful as therapeutic agent for the symptomatic treatment of rheumatoid arthritis as shown by imaging and clinical examination

166 Ho-HA Evaluation as therapeutic agent for rheumatoid arthritis treatment

International Nuclear Information System (INIS)

Chandia, M; Errazu, X; Mendoza, P; Troncoso, F; Jofre, J; Sierralta, P

2003-01-01

Aim: Rheumatoid arthritis is a limiting disease having, among its pathological features, the inflammation of synovial tissue with progressive and later destruction of the articulation. This lead to joint deformation and loss of its function, generating pain and reducing the mobility of the affected articulation. The aim was to evaluate 166 Ho-Hydroxyapatite ( 166 Ho-HA) as potential radiopharmaceutical for the syntomatic treatment of chronic and acute arthritis Materials and Methods: 166 Holmiun was produced by irradiation of Ho 2 O 3 at La Reina Research Reactor, Nuclear Chilean Energy Commission. Hydroxyapatite was in-house synthetized. Its labelling and quality controls follows the internationally accepted procedures. An antigen arthritis was induced to eight New Zealand rabbits with the 166 Ho-HA radiochemical being administred thereafter in two dosage modalities (single and double). The compound therapeutic efficiency was evaluated based upon clinical improvement and images from the inflamated articulation using 67 Ga citrate before and after 166 Ho-HA injection. Results: The radiochemical purity of the innoculated compound was greater than 98% as measured under sterility conditions. Clinically, an inflamation reduction (2 cm), appetite improvement and general well being was observed. The 166 Ho-HA distribution and localization was monitored using gamma camera images taken at 4 and 24 h. There was no evidence of extraarticular leakage. From the 67 Ga citrate imaging, the acute group shows an overall improvement of well being corresponding to a lesser uptake at the inflamated articulation, regarding to the chronic group. The 166 Ho-HA double dosis, compared to the single dosis, suggest a reduced uptake of 67 Ga citrate at the inflamated tissue, meaning an increased therapeutic effect. Conclusions: 166 Ho-HA is usefull as therapeutic agent for the syntomatic treatment of rheumatoideal arthritis as shown by imaging and clinical examination (author)

Mechanical, thermal, rheological and morphological behaviour of irradiated PP/HA composites

International Nuclear Information System (INIS)

Ramirez, C.; Albano, C.; Karam, A.; Dominguez, N.; Sanchez, Y.; Gonzalez, G.

2005-01-01

Hydroxyapatite (HA) reinforced polypropylene (PP) composites are being developed as bone graft materials. In this research, the effect of γ irradiation on mechanical, rheological, thermal and morphological behaviour of PP-HA composites was studied. The melt flow index of polymer increased markedly when it was exposed to radiation. This is indicative of chain scission reaction as the predominant process. During the tensile testing, the composites exhibited brittle behaviour, showing no fluency point. Elongation at break showed a tendency to decrease with the increase in radiation dose while stress at break did not show significant variation with radiation dose. High HA content (>20%) and radiation dose (25 kGy) had significant influence on thermal stability

Enhancement of mucosal immune responses by chimeric influenza HA/SHIV virus-like particles

International Nuclear Information System (INIS)

Guo Lizheng; Lu Xiaoyan; Kang, S.-M.; Chen Changyi; Compans, Richard W.; Yao Qizhi

2003-01-01

To enhance mucosal immune responses using simian/human immunodeficiency virus-like particles (SHIV VLPs), we have produced novel phenotypically mixed chimeric influenza HA/SHIV VLPs and used them to immunize C57BL/6J mice intranasally. Antibody and cytotoxic T-cell (CTL) responses as well as cytokine production in both systemic and mucosal sites were compared after immunization with SHIV VLPs or chimeric HA/SHIV VLPs. By using enzyme-linked immunosorbent assay (ELISA), the levels of serum IgG and mucosal IgA to the HIV envelope protein (Env) were found to be highest in the group immunized with chimeric HA/SHIV VLPs. Furthermore, the highest titer of serum neutralizing antibody against HIV Env was found with the group immunized with chimeric HA/SHIV VLPs. Analysis of the IgG1/IgG2a ratio indicated that a T H 1-oriented immune response resulted from these VLP immunizations. HA/SHIV VLP-immunized mice also showed significantly higher CTL responses than those observed in SHIV VLP-immunized mice. Moreover, a MHC class I restricted T-cell activation ELISPOT assay showed a mixed type of T H 1/T H 2 cytokines in the HA/SHIV VLP-immunized mice, indicating that the chimeric VLPs can enhance both humoral and cellular immune responses to the HIV Env protein at multiple mucosal and systemic sites. The results indicate that incorporation of influenza HA into heterotypic VLPs may be highly effective for targeting vaccines to mucosal surfaces

3D bioprinting of methacrylated hyaluronic acid (MeHA) hydrogel with intrinsic osteogenicity.

Science.gov (United States)

Poldervaart, Michelle T; Goversen, Birgit; de Ruijter, Mylene; Abbadessa, Anna; Melchels, Ferry P W; Öner, F Cumhur; Dhert, Wouter J A; Vermonden, Tina; Alblas, Jacqueline

2017-01-01

In bone regenerative medicine there is a need for suitable bone substitutes. Hydrogels have excellent biocompatible and biodegradable characteristics, but their visco-elastic properties limit their applicability, especially with respect to 3D bioprinting. In this study, we modified the naturally occurring extracellular matrix glycosaminoglycan hyaluronic acid (HA), in order to yield photo-crosslinkable hydrogels with increased mechanical stiffness and long-term stability, and with minimal decrease in cytocompatibility. Application of these tailor-made methacrylated hyaluronic acid (MeHA) gels for bone tissue engineering and 3D bioprinting was the subject of investigation. Visco-elastic properties of MeHA gels, measured by rheology and dynamic mechanical analysis, showed that irradiation of the hydrogels with UV light led to increased storage moduli and elastic moduli, indicating increasing gel rigidity. Subsequently, human bone marrow derived mesenchymal stromal cells (MSCs) were incorporated into MeHA hydrogels, and cell viability remained 64.4% after 21 days of culture. Osteogenic differentiation of MSCs occurred spontaneously in hydrogels with high concentrations of MeHA polymer, in absence of additional osteogenic stimuli. Addition of bone morphogenetic protein-2 (BMP-2) to the culture medium further increased osteogenic differentiation, as evidenced by increased matrix mineralisation. MeHA hydrogels demonstrated to be suitable for 3D bioprinting, and were printed into porous and anatomically shaped scaffolds. Taken together, photosensitive MeHA-based hydrogels fulfilled our criteria for cellular bioprinted bone constructs within a narrow window of concentration.

3D bioprinting of methacrylated hyaluronic acid (MeHA hydrogel with intrinsic osteogenicity.

Directory of Open Access Journals (Sweden)

Michelle T Poldervaart

Full Text Available In bone regenerative medicine there is a need for suitable bone substitutes. Hydrogels have excellent biocompatible and biodegradable characteristics, but their visco-elastic properties limit their applicability, especially with respect to 3D bioprinting. In this study, we modified the naturally occurring extracellular matrix glycosaminoglycan hyaluronic acid (HA, in order to yield photo-crosslinkable hydrogels with increased mechanical stiffness and long-term stability, and with minimal decrease in cytocompatibility. Application of these tailor-made methacrylated hyaluronic acid (MeHA gels for bone tissue engineering and 3D bioprinting was the subject of investigation. Visco-elastic properties of MeHA gels, measured by rheology and dynamic mechanical analysis, showed that irradiation of the hydrogels with UV light led to increased storage moduli and elastic moduli, indicating increasing gel rigidity. Subsequently, human bone marrow derived mesenchymal stromal cells (MSCs were incorporated into MeHA hydrogels, and cell viability remained 64.4% after 21 days of culture. Osteogenic differentiation of MSCs occurred spontaneously in hydrogels with high concentrations of MeHA polymer, in absence of additional osteogenic stimuli. Addition of bone morphogenetic protein-2 (BMP-2 to the culture medium further increased osteogenic differentiation, as evidenced by increased matrix mineralisation. MeHA hydrogels demonstrated to be suitable for 3D bioprinting, and were printed into porous and anatomically shaped scaffolds. Taken together, photosensitive MeHA-based hydrogels fulfilled our criteria for cellular bioprinted bone constructs within a narrow window of concentration.

A Modified ELISA Accurately Measures Secretion of High Molecular Weight Hyaluronan (HA) by Graves' Disease Orbital Cells

Science.gov (United States)

Krieger, Christine C.

2014-01-01

Excess production of hyaluronan (hyaluronic acid [HA]) in the retro-orbital space is a major component of Graves' ophthalmopathy, and regulation of HA production by orbital cells is a major research area. In most previous studies, HA was measured by ELISAs that used HA-binding proteins for detection and rooster comb HA as standards. We show that the binding efficiency of HA-binding protein in the ELISA is a function of HA polymer size. Using gel electrophoresis, we show that HA secreted from orbital cells is primarily comprised of polymers more than 500 000. We modified a commercially available ELISA by using 1 million molecular weight HA as standard to accurately measure HA of this size. We demonstrated that IL-1β-stimulated HA secretion is at least 2-fold greater than previously reported, and activation of the TSH receptor by an activating antibody M22 from a patient with Graves' disease led to more than 3-fold increase in HA production in both fibroblasts/preadipocytes and adipocytes. These effects were not consistently detected with the commercial ELISA using rooster comb HA as standard and suggest that fibroblasts/preadipocytes may play a more prominent role in HA remodeling in Graves' ophthalmopathy than previously appreciated. PMID:24302624

Inhibitory effect of PTD-OD-HA fusion protein on Bcr-Abl in K562 cells

Directory of Open Access Journals (Sweden)

Miao GAO

2012-10-01

Full Text Available Objective　To study the transduction dynamics, location of PTD-OD-HA fusion protein and its interaction with Bcr-Abl oncoprotein in K562 cell lines, and explore the influence of PTD-OD-HA fusion protein on oligomerization and tyrosine kinase activity of Bcr-Abl. Methods　PTD-OD-HA fusion protein was labeled with FITC and co-cultured with K562 cells. The transduction efficiency of labeled PTD-OD-HA at different doses and time intervals was observed under fluorescence microscope. The location of labeled PTD-OD-HA fusion protein in K562 cells was detected by confocal microscopy. The interaction of PTD-OD-HA fusion protein with Bcr-Abl oncoprotein was confirmed by coimmunoprecipitation. The phosphorylation of Bcr-Abl oncoprotein was detected by Western blotting. Results　PTD-OD-HA fusion protein labeled with FITC was transduced into K562 cells in a dose- and time-dependent manner. PTD-OD-HA fusion protein was located in the cytoplasm of K562 cells and was consistent with the location of Bcr-Abl oncoprotein. The interaction of PTD-OD-HA fusion protein with Bcr-Abl oncoprotein was proved in K562 cells. This interaction could interrupt the homologous oligomerization of Bcr-Abl oncoprotein and reduce the phosphorylation of Bcr-Abl oncoprotein. Conclusion　PTD-OD-HA fusion protein could be transduced into K562 cells efficiently, inhibit the oligomerization and reduce the phosphorylation of Bcr-Abl oncoprotein.

Asphaltenes in Mexican fuel oils; Asfaltenos en combustoleos mexicanos

Energy Technology Data Exchange (ETDEWEB)

Longoria Ramirez, Rigoberto [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

1996-12-31

In this article the main aspects in which the Instituto de Investigaciones Electricas (IIE) has worked to contribute to the solution of problems due to the presence of asphaltenes in national fuel oils, are emphasized. The increment of these compounds, that concentrate harmful elements, in the last ten years has reached 22% by weight of the fuel oil. It is demonstrated that the quantification of asphaltenes depends on the type of solvent employed. [Espanol] En este articulo se subrayan los principales aspectos en los que el Instituto de Investigaciones Electricas (IIE) ha trabajado para contribuir a la solucion de problemas debidos a la presencia de asfaltenos en combustoleos nacionales. El incremento de estos compuestos, que concentran elementos nocivos, en los ultimos diez anos ha llegado hasta un 22% del peso del combustoleo. Se demuestra que la cuantificacion de los asfaltenos depende del tipo de solvente utilizado.

Asphaltenes in Mexican fuel oils; Asfaltenos en combustoleos mexicanos

Energy Technology Data Exchange (ETDEWEB)

Longoria Ramirez, Rigoberto [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

1997-12-31

In this article the main aspects in which the Instituto de Investigaciones Electricas (IIE) has worked to contribute to the solution of problems due to the presence of asphaltenes in national fuel oils, are emphasized. The increment of these compounds, that concentrate harmful elements, in the last ten years has reached 22% by weight of the fuel oil. It is demonstrated that the quantification of asphaltenes depends on the type of solvent employed. [Espanol] En este articulo se subrayan los principales aspectos en los que el Instituto de Investigaciones Electricas (IIE) ha trabajado para contribuir a la solucion de problemas debidos a la presencia de asfaltenos en combustoleos nacionales. El incremento de estos compuestos, que concentran elementos nocivos, en los ultimos diez anos ha llegado hasta un 22% del peso del combustoleo. Se demuestra que la cuantificacion de los asfaltenos depende del tipo de solvente utilizado.

Ahmet Haşim’de Ev-Yuva ve Doğa İmgesi Home-Nest and Nature Images in Ahmet Haşim

Directory of Open Access Journals (Sweden)

Refika ALTIKULAÇ DEMİRDAĞ

2012-12-01

Full Text Available Home and Nest images are commonly used images in poetry. Home is a shelter within the poet feels himself safe and secure. Image of nature in Ahmet Hasim’s poems, has been the most focused subjects on. Furthermore, assessments have been made on the poet's pessimism and desire that escape to an imaginary world from the real world. But, It hasn’t been dwelled enough on subject why Haşim escape from the real world to an imaginary world. Because, imaginary world that was created in Haşim’s poems, is richer than the real world. It is alsa a remarkable issue, the need of Haşim for a home and a nest or a shelter in nature. Haşim is more peaceful in a narrower space, inside the home. We can see this situation in poet’s poem called "Evim". Furthermore Hashim described a larger shelter in his poem called "O Belde”. “O Belde” is a part of nature and a nook in nature. Therefore, just like abird's nest, it has been made with the poet's feelings, his heart. Actuallyit is not important to be real or not for poet. Because in fact the poetknows the place that described, is mind’s shelter. It is the poet’s mindand dream, who wants to escape from the real world. His mind wants toescape from the real world, Because the real world is a place that ispessimistic and unsafe. There is plenty of "movement" and plenty oflight. This plenty of movement and light has scared the poet. He canfind the world that is less bright, more silent, more and more intimatewith nature, in his "untouched" regions of his mind. This is the placethat he found is home-nest, “O Belde”. Ev ve yuva imgeleri şiirde çok kullanılan imgelerdendir. Şairin kendini güvende hissettiği bir sığınaktır ev. Ahmet Haşim’in şiirlerinde doğa imgesi üzerinde en fazla durulan konulardan olmuştur. Ayrıca şairin karamsarlığı ve gerçek dünyadan hayali dünyaya kaçma isteği üzerinde de değerlendirmeler yapılmıştır. Fakat Haşim’in neden gerçek d

Aportes y tiempos del texto (de teatro

Directory of Open Access Journals (Sweden)

Jean-Frédéric Chevallier

2018-01-01

Full Text Available Volviendo a visitar, a la luz de las prácticas escénicas contemporáneas, la relación entre texto y teatro, se propone, para entender los matices de dicha relación, hablar de texto-pregunta, texto débil, texto fuerte, texto saturado e, in fine, texto-propuesta. El surgimiento de este último, el texto-propuesta, producido por el espectador, invita a pensar que si bien se ha hablado mucho del “devenir-escénico” de un texto literario, quizás haya llegado el tiempo de enfocar el “devenir-literatura” de un acto teatral.

El profesor, principal agente de la motivación

OpenAIRE

Castellano, Almudena

2012-01-01

En la actualidad, hay abiertas numerosas investigaciones acerca de la educación y del sistema. Dichos estudios parten de la realidad del fracaso escolar tan alto que se encuentra en las aulas, y de la realidad que sitúa a la educación española en niveles inferiores que la mayoría de países europeos. Lo que buscan es encontrar la solución a este problema, pero aún no se ha llegado a un consenso en relación a cuáles son las causas y qué medidas se pueden tomar para mejorar el sistema educativo....

PRESENTACIÓN

Directory of Open Access Journals (Sweden)

Miguel Lisbona Guillén

2006-12-01

Full Text Available La práctica de actividades físico-deportivas ocupa, hoy en día, buena parte del tiempo de ocio de un alto porcentaje de la población en nuestras sociedades; y no sólo eso, sino que ha llegado a convertirse en un importante referente simbólico y cultural para determinados grupos sociales. Más evidente aún resulta la fuerza expansiva de sus universos simbólicos en la representación de espectáculos deportivos como el fútbol, convertido en un nuevo lenguaje universal, en la “nueva religión” de buena parte de las sociedades modernas. ...

Experimental study on bone tissue reaction around HA implants radiated after implantation

International Nuclear Information System (INIS)

Kudo, Masato; Matsui, Yoshiro; Tamura, Sayaka; Chen, Xuan; Uchida, Haruo; Mori, Kimie; Ohno, Kohsuke; Michi, Ken-ichi

1998-01-01

This study was conducted to investigate histologically and histomorphometrically the tissue reaction around hydroxylapatite (HA) implants that underwent irradiation in 3 different periods in the course of bone healing after implantation. The cylindrical high-density HA implants were implanted in 48 Japanese white rabbit mandibles. A single 15 Gy dose was applied to the mandible 5, 14, or 28 days after implantation. The rabbits were sacrificed 7, 14, 28, and 90 days after irradiation. Nonirradiated rabbits were used as controls. CMR, labeling with tetracycline and calcein, and non-decalcified specimens stained with toluidine blue were used for histological analyses and histomorphometric measurements. The results were as follows: In the rabbits irradiated 5 days after implantation, the HA-bone contact was observed later than that in the controls and the bone-implant contact surface ratio was lower than that in the controls at examination because necrosis of the newly-formed bone occurred just after irradiation. HA-bone contact of the rabbits irradiated 14 and 28 days after implantation was similar to that of the controls. And, bone remodeling was suppressed in rabbits of each group sacrificed at 90 days after irradiation. The results suggested that a short interval between implantation and irradiation causes direct contact between HA implant and bone and a long lapse of time before irradiation hardly affects the bone-implant contact, but delays bone remodeling. Therefore, it is necessary to prevent overloading the HA implants irradiated after implantation and pay utmost attention to conditions around the bone-implant contact. (author)

New therapeutic agent for radiation synovectomy - preparation of {sup 166}Ho-EDTMP-HA particle

Energy Technology Data Exchange (ETDEWEB)

Bai, H.; Jin, X.; Du, J.; Wang, F.; Chen, D.; Fan, H.; Cheng, Z.; Zhang, J. [China Institute of Atomic Energy, Beijing (Switzerland). Isotope Department

1997-10-01

In order to prepare new therapeutical agent for radiation synovectomy, Hydroxyapatite (HA) was labelled with {sup 166}Ho by EDTMP that had high affinity to HA particles. Radiolabelling of HA particles was divided into two steps, {sup 166}Ho-EDTMP was prepared first; then mixed with HA particles completely and vibrated for 15 minutes on the micromixer at room temperature, washed 3 times with deionized water. Radiolabelling particle was separated from free {sup 166}Ho via centrifugation to determine its radiolabelling efficiency. {sup 166}Ho-EDTMP-HA and {sup 166}Ho-EDTMP were injected into knee joint of normal rabbits respectively, every group was killed at different time postinjection, took out major organ and collected urine and blood, then weighted and determined their radio counts. HA particles, as a natural component of bone was known to have good compatibility with soft tissue and biodegrade into calcium and phosphate in vivo. It was readily prepared from common chemical and formed into particles of desired size range in a controlled process, it had high stability in vitro and vivo. Radiolabelling of HA particle with {sup 166}Ho by EDTMP was simple to perform and provides an excellent labelling yield that was more than 95% under the optimal labelling condition. The optimal labelling condition at room temperature was pH 6.0-8.0 and vibration time 15 minutes. The absorbed capacity of HA particle was 5 mg Ho/g HA particle and size of radiolabelling particle was at range of 2-5,{mu}m that is suitable for therapy of radiation synovectomy. {sup 166}Ho-EDTMP-HA particle demonstrated high in vitro stability in either normal saline or 1% BSA solution, but instability under extremely acidic condition (pH 1-2). The control studies performed with {sup 166}Ho-EDTMP not bound to HA particle provided information on the distribution of radioactivity that would occur upon leakage of the radiochemical compound from joint. Its short half-life, its extremely low leakage from the

Sintering behavior and property of bioglass modified HA-Al2O3 composite

Directory of Open Access Journals (Sweden)

Wang Li-li

2012-01-01

Full Text Available The bioglass modified HA-Al2O3 composites were successfully fabricated by mixing HA, synthesized by wet chemical method between precursor materials H3PO4 and Ca(OH2, with 25wt% Al2O3 and different content of bioglass (5%, 25%, 45%, 65wt% respectively, with a mole fraction of 53.9%SiO2, 22.6%Na2O, 21.8%CaO, and 1.7wt%P2O5, sintered in air at various temperatures (750-950°C for 2h. when the content of bioglass is below 45wt% in the composite, HA decomposes completely and transforms to β-TCP. The main phase in this case are β-TCP, Al2O3 and Ca3(AlO32.When the content of bioglass is above 45wt% in the composite, the decomposition of HA to β-TCP is suppressed and the main phases in this case are Al2O3 and HA, DCP□CaHPO4□ and β-TCP, which almost have the same chemical composition, forming ternary-glass phase, and have better bioactive than pure HA. It can also be found that at the certain addition of bioglass, the higher sintered temperature, the bigger volume density and flexural strength of the composite are, but when the sintered temperature reaches 950°C, they decrease. This modified HA-Al2O3 composites by calcium silicate glass have a much lower sintering temperature and decrease the production cost much.

HaVec: An Efficient de Bruijn Graph Construction Algorithm for Genome Assembly

Directory of Open Access Journals (Sweden)

Md Mahfuzer Rahman

2017-01-01

Full Text Available Background. The rapid advancement of sequencing technologies has made it possible to regularly produce millions of high-quality reads from the DNA samples in the sequencing laboratories. To this end, the de Bruijn graph is a popular data structure in the genome assembly literature for efficient representation and processing of data. Due to the number of nodes in a de Bruijn graph, the main barrier here is the memory and runtime. Therefore, this area has received significant attention in contemporary literature. Results. In this paper, we present an approach called HaVec that attempts to achieve a balance between the memory consumption and the running time. HaVec uses a hash table along with an auxiliary vector data structure to store the de Bruijn graph thereby improving the total memory usage and the running time. A critical and noteworthy feature of HaVec is that it exhibits no false positive error. Conclusions. In general, the graph construction procedure takes the major share of the time involved in an assembly process. HaVec can be seen as a significant advancement in this aspect. We anticipate that HaVec will be extremely useful in the de Bruijn graph-based genome assembly.

Elastin-like protein-hyaluronic acid (ELP-HA) hydrogels with decoupled mechanical and biochemical cues for cartilage regeneration.

Science.gov (United States)

Zhu, Danqing; Wang, Huiyuan; Trinh, Pavin; Heilshorn, Sarah C; Yang, Fan

2017-05-01

Hyaluronic acid (HA) is a major component of cartilage extracellular matrix and is an attractive material for use as 3D injectable matrices for cartilage regeneration. While previous studies have shown the promise of HA-based hydrogels to support cell-based cartilage formation, varying HA concentration generally led to simultaneous changes in both biochemical cues and stiffness. How cells respond to the change of biochemical content of HA remains largely unknown. Here we report an adaptable elastin-like protein-hyaluronic acid (ELP-HA) hydrogel platform using dynamic covalent chemistry, which allows variation of HA concentration without affecting matrix stiffness. ELP-HA hydrogels were created through dynamic hydrazone bonds via the reaction between hydrazine-modified ELP (ELP-HYD) and aldehyde-modified HA (HA-ALD). By tuning the stoichiometric ratio of aldehyde groups to hydrazine groups while maintaining ELP-HYD concentration constant, hydrogels with variable HA concentration (1.5%, 3%, or 5%) (w/v) were fabricated with comparable stiffness. To evaluate the effects of HA concentration on cell-based cartilage regeneration, chondrocytes were encapsulated within ELP-HA hydrogels with varying HA concentration. Increasing HA concentration led to a dose-dependent increase in cartilage-marker gene expression and enhanced sGAG deposition while minimizing undesirable fibrocartilage phenotype. The use of adaptable protein hydrogels formed via dynamic covalent chemistry may be broadly applicable as 3D scaffolds with decoupled niche properties to guide other desirable cell fates and tissue repair. Copyright © 2017 Elsevier Ltd. All rights reserved.

A Study of Hybrid Composite Hydroxyapatite (HA-Geopolymers as a Material for Biomedical Application

Directory of Open Access Journals (Sweden)

Saleha

2017-01-01

Full Text Available The main purpose of this research is to study the physical properties and microstructure characters of hybrid composites HA-geopolymers as a material for biomedical application. Hybrid composite HA–geopolymers were produced through alkaline activation method of metakaolin as a matrix and HA as the filler. HA was synthesized from eggshell particles by using a precipitation method. The addition of HA in metakaolin paste was varied from 0.5%, 1.0%, 1.5%, and 2.0% relative the weight of metakaolin. FTIR was used to examine the absorption bands the composites. X-ray diffraction (XRD was used to study the crystal structure of the starting and the resulting materials. Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS was used to investigate the surface morphology of the composites. The thermal properties of the samples was examined by means of Differential Scanning Calorimetry (DSC. Capacitance measurement was conducted to investigate the bioactive properties of HA. The study results suggest that hybrid composite HA-geopolymers has a potential to be applied as a biomedical such as biosensor material.

The Bologna process - governing higher education in Europe through standardisation

Directory of Open Access Journals (Sweden)

Andreas Fejes

2006-01-01

conocimiento, empleabilidad, aprendizaje de por vida, garantía de calidad y movilidad. Crean una corriente de pensamiento que facilita la contemplación del proceso de Bolonia como la manera más racional de actuar. En la segunda parte, el proceso se estudia como una técnica de estandarización, que causa cierta tensión en referencia a los ideales de libertad. Se crean exclusiones; aquellos paises que optan por no participar, son denominados como "los otros", necesitados de un remedio. En la tercera y última parte, vemos cómo tales ideas se adaptan o resultan conflictivas con las distintas creencias educativas locales. Aunque las narrativas producidas en Suecia, en su mayor parte parecen adaptarse al proceso, ha surgido un sueco "específico". Actúa como una técnica persuasiva para la implementación de las ideas surgidas de la Declaración de Bolonia

Bolonia 2º año: Los estudios universitarios de Relaciones Públicas en Cataluña (curso 2011-12 / Bologna 2nd year: The university studies of Publics Relations in Catalonia (course 2011-12

Directory of Open Access Journals (Sweden)

Kathy Matilla

2012-12-01

Full Text Available ResumenEste artículo describe los estudios universitarios del área de Relaciones Públicas en los Grados de Publicidad y Relaciones Públicas del sistema universitario de Cataluña, en el curso 2011-12.En el marco teórico y en la discusión se describen los más recientes estudios desarrollados por asociaciones profesionales europeas y españolas especializadas en Relaciones Públicas (2010-11, que permiten contextualizar la situación y constatar el abismo existente entre las necesidades formativas para un correcto desempeño de la praxis profesional y las escasas y deficientes competencias específicas que, sobre Relaciones Públicas, pueden adquirir los futuros graduados con los actuales programas formativos ofertados por las universidades catalanas. La investigación se ha llevado a cabo mediante un rastreo sistemático de las páginas web de las universidades de la unidad de análisis.De la investigación se desprende la ausencia de cambios notables en los grados con respecto al modelo universitario anterior a la implantación del Proceso de Bolonia (Licenciaturas, manteniéndose como una oferta deficiente, en línea con lo constatado los cursos 2009-10 y 2010-11, a partir de la implantación del EEES. Se concluye que las Relaciones Públicas siguen siendo minoritarias en cuanto a número de asignaturas con respecto a las específicas de Publicidad y al planteamiento general de las mismas, continuan confinadas a un segundo plano y poseen tintes mayoritariamente instrumentales (tácticos y no estratégicos.AbstractThis article describes the university studies of Public Relations in Advertising and Public Relations degrees from the university system of Catalonia, during the course 2011-12.The theoretical framework and the discussion describes the most recent European an Spanish studies conducted by professional associations specialized in Public Relations (2010-11, which allow to contextualize the situation and assess the gap between the

{sup 166} Ho-HA Evaluation as therapeutic agent for rheumatoid arthritis treatment; Evaluacion de {sup 166}Ho-Ha como agente terapeutico en el tratamiento de la artritis reumatoidea

Energy Technology Data Exchange (ETDEWEB)

Chandia, M; Errazu, X [Comision Chilena de Energia Nuclear, Santiago (Chile); Mendoza, P [Departamento de Medicina Nuclear, Hospital Militar, Santiago (Chile); Troncoso, F [Comision Chilena de Energia Nuclear, Santiago (Chile); Jofre, J; Sierralta, P [Departamento de Medicina Nuclear, Hospital Militar, Santiago (Chile)

2003-01-01

Aim: Rheumatoid arthritis is a limiting disease having, among its pathological features, the inflammation of synovial tissue with progressive and later destruction of the articulation. This lead to joint deformation and loss of its function, generating pain and reducing the mobility of the affected articulation. The aim was to evaluate {sup 166}Ho-Hydroxyapatite ({sup 166} Ho-HA) as potential radiopharmaceutical for the syntomatic treatment of chronic and acute arthritis Materials and Methods: {sup 166}Holmiun was produced by irradiation of Ho{sub 2}O{sub 3} at La Reina Research Reactor, Nuclear Chilean Energy Commission. Hydroxyapatite was in-house synthetized. Its labelling and quality controls follows the internationally accepted procedures. An antigen arthritis was induced to eight New Zealand rabbits with the {sup 166}Ho-HA radiochemical being administred thereafter in two dosage modalities (single and double). The compound therapeutic efficiency was evaluated based upon clinical improvement and images from the inflamated articulation using {sup 67}Ga citrate before and after {sup 166} Ho-HA injection. Results: The radiochemical purity of the innoculated compound was greater than 98% as measured under sterility conditions. Clinically, an inflamation reduction (2 cm), appetite improvement and general well being was observed. The {sup 166} Ho-HA distribution and localization was monitored using gamma camera images taken at 4 and 24 h. There was no evidence of extraarticular leakage. From the {sup 67}Ga citrate imaging, the acute group shows an overall improvement of well being corresponding to a lesser uptake at the inflamated articulation, regarding to the chronic group. The {sup 166}Ho-HA double dosis, compared to the single dosis, suggest a reduced uptake of {sup 67}Ga citrate at the inflamated tissue, meaning an increased therapeutic effect. Conclusions: {sup 166} Ho-HA is usefull as therapeutic agent for the syntomatic treatment of rheumatoideal arthritis

Hamster endogenous retrovirus (HaER) - distinct properties of structural proteins and DNA polymerase

International Nuclear Information System (INIS)

Goldschmied-Reouven, A.; Yaniv, A.

1983-01-01

The structural proteins as well as some features of the RNA-dependent DNA polymerase of the hamster endogenous retrovirus (HaER) were examined. The polypeptide pattern of this virus is substantially different from that of other known retroviruses in containing major polypeptides with molecular weights of 68000, 59000, 27000, 24000 daltons. Double antibody competitive radioimmunoassays showed that the HaER particles do not share any detectable antigenic relatedness with the murine viruses' p30, but manifest a considerable relatedness with the feline leukemia virus p27 and a slight cross-reactivity with the rat virus major protein. The RNA-dependent DNA polymerase of HaER virus has a molecular size of approximately 73000 daltons and in contrast to other mammalian retroviruses shows no significant preference for Mn 2+ over Mg 2+ . Apart from the lack of antigenic relatedness between the HaER virus proteins and the p30 protein of murine viruses, there is also no antigenic relatedness between HaER and murine viruses insofar as their DNA polymerase is concerned. (Author)

Läkaköha - aktuaalne uurimisteema / Marje Oona

Index Scriptorium Estoniae

Oona, Marje, 1963-

2012-01-01

TÜ peremeditsiini õppetooli töötajate poolt algatatud uurimistööst, mille eesmärgiks on uurida Bordetella spp. infektsioonide epidemioloogiat, molekulaargeneetikat ja kliinilisi eripärasid ning selgitada läkaköha sagedasema diagnsimise põhjusi Eestis

The Technology and Properties of Digital Double Pulse Electrodepositing Ni-HA Composite Coating of Bioceramics

Institute of Scientific and Technical Information of China (English)

DONG He-yan; WANG Zhou; SHI Gu-guizhi; FU Chuan-qi; CHEN Wei-rong; JIN Zhong-hong; LI Yan

2004-01-01

This article discusses and analyses the technology, the surface image, microstructure and ability of digital double pulse electrodepositing Ni-HA composite coatings of bioceramics made on 1Crl8Ni9Ti substrate by SEM ,XRD and so on. The results shows that ( 1 ) the HA particles exit in substrate uniformly; (2) XRD result shows that there are HA peaks at 78. 023 ° ,43. 246°and 73. 120°differently; (3) The microhardnees of the composite coatings is increased with the rise of content of HA particles, and on the same conditions the microhardnees value is greater than that of common non-pulse electrodepositing Ni-HA composite coatings of bioceramics. (4) The grain size of digital double pulse electrodepositing Ni-HA composite coatings of bioceramics is much thinner than that of common D. C.

c-Ha-ras BamHI RFLP in human urothelial tumors and point mutations in hot codons

International Nuclear Information System (INIS)

Weismanova, E; Skovraga, M.; Kaluz, S.

1993-01-01

High-molecular weights DNAs from 30 bladder and renal cell carcinomas (RCC) were isolated and the c-Ha-ras the c-Ha-ras gene BamHI RFLP was examined. Amplification of c-Ha-ras with normal localization with regard to the size of alleles was found only in the case. One of the normally localized c-Ha-ras allele termed RCC c-H-ras of a length of about 6.6 kbp was cloned and an oncogene-activating point mutation was identified using two restriction enzymes. After comparison of CfrI and Cfr10I cleavage maps of RCC c-Ha-ras to complete nucleotide sequences of EJ/T24 c-Ha-ras oncogene and its normal counterpart, a point mutation was identified within codon 11 or 12. The use of CfrI and Cfr10I is of value for clinical practice in identification of point mutations in c-Ha-ras PCR product in neoplasia accompanied by somatic mutation of c-Ha-ras. The correlation among c-Ha-ras allele, amplification/loss, presence of point mutation and progression of neoplasia is discussed. (author)

LA PESCA COMO FACTOR DE DESARROLLO DEL TURISMO SOSTENIBLE. EL CASO DE ÁGUILAS (MURCIA

Directory of Open Access Journals (Sweden)

Pedro Pérez Piernas

2012-01-01

Full Text Available De los municipios del litoral murciano, el de Águilas ha sido el que menos ha desarrollado la actividad turística, debido a que ha centrado su economía en la agricultura y la pesca. No por ello ha quedado al margen del proceso turístico urbanizador que ha afectado al Arco Mediterráneo en la pasada década, aunque no ha llegado a desarrollar su macroproyecto turístico de Marina de Cope. Entre mayo de 2004 y 2006, Águilas, junto a otros puertos pesqueros del Mediterráneo Occidental, participa en el proyecto MARIMED: la pesca como factor de desarrollo del turismo sostenible, iniciativa INTERREG III-B, cuyo fin es el desarrollo de formas de turismo teniendo como elemento central e innovador la pesca, considerada como sistema social, económico y cultural, capaz de ofrecer un valor añadido al beneficio del mar. Con los estudios realizados en el proyecto MARIMED se recogen los materiales necesarios para la puesta en valor del la pesca como recurso turístico en Águilas. Sobre esa base se pone en marcha en 2009 el Plan de Competitividad Turística de Águilas, el Mar y la Pesca. Con esta aportación se pretende analizar el desarrollo de un producto turístico nuevo sobre un espacio donde la tradición pesquera ha dejado una huella importante en su paisaje y cultura.

Fabrication and in vitro biocompatibility of biomorphic PLGA/nHA composite scaffolds for bone tissue engineering

International Nuclear Information System (INIS)

Qian, Junmin; Xu, Weijun; Yong, Xueqing; Jin, Xinxia; Zhang, Wei

2014-01-01

In this study, biomorphic poly(DL-lactic-co-glycolic acid)/nano-hydroxyapatite (PLGA/nHA) composite scaffolds were successfully prepared using cane as a template. The porous morphology, phase, compression characteristics and in vitro biocompatibility of the PLGA/nHA composite scaffolds and biomorphic PLGA scaffolds as control were investigated. The results showed that the biomorphic scaffolds preserved the original honeycomb-like architecture of cane and exhibited a bimodal porous structure. The average channel diameter and micropore size of the PLGA/nHA composite scaffolds were 164 ± 52 μm and 13 ± 8 μm, respectively, with a porosity of 89.3 ± 1.4%. The incorporation of nHA into PLGA decreased the degree of crystallinity of PLGA, and significantly improved the compressive modulus of biomorphic scaffolds. The in vitro biocompatibility evaluation with MC3T3-E1 cells demonstrated that the biomorphic PLGA/nHA composite scaffolds could better support cell attachment, proliferation and differentiation than the biomorphic PLGA scaffolds. The localization depth of MC3T3-E1 cells within the channels of the biomorphic PLGA/nHA composite scaffolds could reach approximately 400 μm. The results suggested that the biomorphic PLGA/nHA composite scaffolds were promising candidates for bone tissue engineering. - Highlights: • Novel biomimetic PLGA/nHA composite scaffolds were successfully prepared. • nHA addition improved elastic modulus of PLGA scaffold and decreased its crystallinity. • PLGA/nHA composite scaffolds had better biocompatibility than PLGA scaffolds. • Biomorphic PLGA/nHA composite scaffold had great potential in bone tissue engineering

Fabrication and in vitro biocompatibility of biomorphic PLGA/nHA composite scaffolds for bone tissue engineering

Energy Technology Data Exchange (ETDEWEB)

Qian, Junmin, E-mail: jmqian@mail.xjtu.edu.cn; Xu, Weijun; Yong, Xueqing; Jin, Xinxia; Zhang, Wei

2014-03-01

In this study, biomorphic poly(DL-lactic-co-glycolic acid)/nano-hydroxyapatite (PLGA/nHA) composite scaffolds were successfully prepared using cane as a template. The porous morphology, phase, compression characteristics and in vitro biocompatibility of the PLGA/nHA composite scaffolds and biomorphic PLGA scaffolds as control were investigated. The results showed that the biomorphic scaffolds preserved the original honeycomb-like architecture of cane and exhibited a bimodal porous structure. The average channel diameter and micropore size of the PLGA/nHA composite scaffolds were 164 ± 52 μm and 13 ± 8 μm, respectively, with a porosity of 89.3 ± 1.4%. The incorporation of nHA into PLGA decreased the degree of crystallinity of PLGA, and significantly improved the compressive modulus of biomorphic scaffolds. The in vitro biocompatibility evaluation with MC3T3-E1 cells demonstrated that the biomorphic PLGA/nHA composite scaffolds could better support cell attachment, proliferation and differentiation than the biomorphic PLGA scaffolds. The localization depth of MC3T3-E1 cells within the channels of the biomorphic PLGA/nHA composite scaffolds could reach approximately 400 μm. The results suggested that the biomorphic PLGA/nHA composite scaffolds were promising candidates for bone tissue engineering. - Highlights: • Novel biomimetic PLGA/nHA composite scaffolds were successfully prepared. • nHA addition improved elastic modulus of PLGA scaffold and decreased its crystallinity. • PLGA/nHA composite scaffolds had better biocompatibility than PLGA scaffolds. • Biomorphic PLGA/nHA composite scaffold had great potential in bone tissue engineering.

Carta de los editores del número

Directory of Open Access Journals (Sweden)

Gloria J. Rosique Cedillo

2014-05-01

Full Text Available Desde la implantación de la reforma educativa del Proceso de Bolonia en el año 2010, las universidades europeas han adaptado sus planes de estudio, sus metodologías docentes, sus sistemas de evaluación, han reconfigurado sus plantillas y realizado cambios en la administración de sus infraestructuras, lo que ha significado una trasformación global en su gestión interna y procesos.Si bien hoy en día el Espacio Europeo de Educación Superior (EEES es una realidad para las instituciones universitarias europeas, este nuevo modelo educativo continúa suponiendo retos y perspectivas a analizar que coadyuven al  fortalecimiento de la puesta en práctica de las principales directrices marcadas en el Proceso de Bolonia. Por tanto, y tras cuatro años de su implantación, resulta necesario hacer un balance crítico-constructivo de la puesta en práctica del EEES y reflexionar sobre las nuevas oportunidades y perspectivas que se vislumbran en el desarrollo del sistema educativo superior en Europa.Por ello, en este tercer número RIESED, Revista Internacional de Estudios sobre Sistemas Educativos, dedica especialmente su atención al Proceso de Bolonia en el contexto de la crisis y a las perspectivas y retos del Espacio Europeo de Educación Superior. Asimismo, y desde un enfoque multidisciplinar, reflexiona acerca de los sistemas de educación en Latinoamérica y de sus principales desafíos y problemáticas.Este tercer número de RIESED cuenta con once trabajos representativos de las diferentes áreas de estudio que se ha propuesto esta publicación. Entre ellos destaca el estudio de caso de Rusia en el EEES;  la movilidad académica en Europa a través del programa ERASMUS; los procesos de reconversión de asignaturas concretas del área de Periodismo: “La Noticia” y “Periodismo Internacional”; un ejemplo de innovación educativa a través del uso del videoblog y una experiencia docente de Lifelong Learning aplicada en la Universidad de

Fabrication and Characterization of Collagen-Immobilized Porous PHBV/HA Nano composite Scaffolds for Bone Tissue Engineering

International Nuclear Information System (INIS)

Jin-Young, B.; Zhi-Cai, X.; Giseop, K.; Keun-Byoung, Y.; Soo-Young, P.; Lee, S.P.; Inn-Kyu, K.

2012-01-01

The porous composite scaffolds (PHBV/HA) consisting of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and hydroxyapatite (HA) were fabricated using a hot-press machine and salt-leaching. Collagen (type I) was then immobilized on the surface of the porous PHBV/HA composite scaffolds to improve tissue compatibility. The structure and morphology of the collagen-immobilized composite scaffolds (PHBV/HA/Col) were investigated using a scanning electron microscope (SEM), Fourier transform infrared (FTIR), and electron spectroscopy for chemical analysis (ESCA). The potential of the porous PHBV/HA/Col composite scaffolds for use as a bone scaffold was assessed by an experiment with osteoblast cells (MC3T3-E1) in terms of cell adhesion, proliferation, and differentiation. The results showed that the PHBV/HA/Col composite scaffolds possess better cell adhesion and significantly higher proliferation and differentiation than the PHBV/HA composite scaffolds and the PHBV scaffolds. These results suggest that the PHBV/HA/Col composite scaffolds have a high potential for use in the field of bone regeneration and tissue engineering.

Mishima jo ha kyu

Directory of Open Access Journals (Sweden)

Matteo Casari

2012-01-01

Full Text Available Abstract – IT La grandezza artistica di Mishima si è espressa attraverso una eterogenea gamma di linguaggi tra i quali il teatro – nella drammaturgia ma anche nella saggistica, regia, recitazione e direzione di compagnia – ha avuto un ruolo di primo piano. Al pari di un uomo di scena Mishima inaugura, a partire dagli anni ’50, un processo di attenzione al proprio corpo come possibile, anzi necessario, veicolo di estrinsecazione etico-estetica tanto da poter istituire l’equazione corpo-teatro quale snodo profondo del suo processo creativo. Il corpo sognato e ottenuto da Mishima attraverso l’addestramento nel kendo, in altre arti marziali e nel body building si conformava ad un ideale estetico di matrice greco-classica assai lontano dal corpo teatrale nipponico. Il corpo come luogo di elaborazione e strumento di espressione autentica da realizzare con impegno, però, lo legano profondamente alle esperienze nascenti – tra gli anni ’50 e ’60 – delle avanguardie teatrali giapponesi. La metafora teatrale è spesso usata nella lettura critica del Mishima uomo e artista con accezione deteriore: un personaggio che dà spettacolo di sé con ripetute provocazioni tra le quali il suicidio del 25 novembre 1970 non sarebbe che l’esempio ultimo e più estremo. La costruzione di sé come personaggio, invece, sembrerebbe corrispondere ad una ben più profonda e meditata necessità di comporre la propria vita in una sapiente messa in scena di classica perfezione: i principi della scansione ritmico formale del jo ha kyu, pilastro teorico del teatro no codificato da Zeami tra XIV e XV secolo, offrono un valido modello di riferimento. Abstract – EN Mishima’s artistic greatness has been expressed through a diverse range of languages among wich theater – in dramaturgy as well as in written essays, as a director, performer and in company direction – played a central role. As a true front-man, Mishima – starting from the 50’s �

Tricalcium phosphate/hydroxyapatite (TCP-HA) bone scaffold as potential candidate for the formation of tissue engineered bone.

Science.gov (United States)

Sulaiman, Shamsul Bin; Keong, Tan Kok; Cheng, Chen Hui; Saim, Aminuddin Bin; Idrus, Ruszymah Bt Hj

2013-06-01

Various materials have been used as scaffolds to suit different demands in tissue engineering. One of the most important criteria is that the scaffold must be biocompatible. This study was carried out to investigate the potential of HA or TCP/HA scaffold seeded with osteogenic induced sheep marrow cells (SMCs) for bone tissue engineering. HA-SMC and TCP/HA-SMC constructs were induced in the osteogenic medium for three weeks prior to implantation in nude mice. The HA-SMC and TCP/HA-SMC constructs were implanted subcutaneously on the dorsum of nude mice on each side of the midline. These constructs were harvested after 8 wk of implantation. Constructs before and after implantation were analyzed through histological staining, scanning electron microscope (SEM) and gene expression analysis. The HA-SMC constructs demonstrated minimal bone formation. TCP/HA-SMC construct showed bone formation eight weeks after implantation. The bone formation started on the surface of the ceramic and proceeded to the centre of the pores. H&E and Alizarin Red staining demonstrated new bone tissue. Gene expression of collagen type 1 increased significantly for both constructs, but more superior for TCP/HA-SMC. SEM results showed the formation of thick collagen fibers encapsulating TCP/HA-SMC more than HA-SMC. Cells attached to both constructs surface proliferated and secreted collagen fibers. The findings suggest that TCP/HA-SMC constructs with better osteogenic potential compared to HA-SMC constructs can be a potential candidate for the formation of tissue engineered bone.

A study on improving mechanical properties of porous HA tissue engineering scaffolds by hot isostatic pressing

International Nuclear Information System (INIS)

Zhao Jing; Xiao Suguang; Lu Xiong; Wang Jianxin; Weng Jie

2006-01-01

Various interconnected porous hydroxyapatite (HA) ceramic scaffolds are universally used to induct the tissue growth for bone repair and replacement, and serve to support the adhesion, transfer, proliferation and differentiation of cells. Impregnation of polyurethane sponges with a ceramic slurry is adopted to produce highly porous HA ceramic scaffolds with a 3D interconnected structure. However, high porosity always accompanies a decrease in the strength of the HA ceramic scaffolds. Therefore, it is significant to improve the strength of the HA ceramic scaffolds with highly interconnected porosity so that they are more suitable in clinical applications. In this work, highly porous HA ceramic scaffolds are first produced by the polymer impregnation approach, and subsequently further sintered by hot isostatic pressing (HIP). The phase composition, macro- and micro-porous structure, sintering and mechanical properties of the porous HA scaffolds are investigated by x-ray diffraction (XRD), scanning electron microscopy (SEM), nanoindentation analysis and compressive test. The experimental results show that the nanohardness and compressive strength of HIP-sintered porous HA ceramics are higher than those of commonly sintered HA scaffolds. The HIP technique can effectively improve the sintering property and densification of porous HA ceramic scaffolds, so inducing an increase in the compression strength

Trehalose improves cell proliferation and dehydration tolerance of human HaCaT cells

Directory of Open Access Journals (Sweden)

Lee Kyung Eun

2015-01-01

Full Text Available Trehalose is a disaccharide molecule that serves as a natural osmotic regulator in halophilic microorganisms and plants but not in mammals. We observed that human HaCaT cells supplied with trehalose improved cell proliferation and extended viability under dehydration. In HaCaT cells, in response to increasing concentrations of exogenous trehalose, the levels of heat shock protein (HSP 70 increased and matrix metalloproteinase (MMP 1 decreased. Proteome analysis of trehalose-treated HaCaT cells revealed remarkable increases in the levels of proteins involved in cell signaling and the cell cycle, including p21 activated kinase I, Sec I family domain protein and elongation factor G. Moreover, the proteins for cell stress resistance, tryptophan hydroxylase, serine/cysteine proteinase inhibitors and vitamin D receptors were also increased. In addition, the proteins responsible for the maintenance of the cytoskeleton and cellular structures including procollagen-lysine dioxygenase, vinculin and ezrin were increased. Proteomic data revealed that trehalose affected HaCaT cells by inducing the proteins involved in cell proliferation. These results suggest that trehalose improves the proliferation and dehydration tolerance of HaCaT cells by inducing proteins involved in cell growth and dehydration protection.

¿Ha cambiado la esencia de la medicina con los adelantos modernos?

Directory of Open Access Journals (Sweden)

Pablo Arango Restrepo

2013-01-01

Full Text Available La sociedad a lo largo de los siglos ha tenido cambios progresivos y en la actualidad estos son rápidos e incluyen el ejercicio de la profesión médica, que ha perdido su enfoque inicial en el paciente para centrarse en un interés prin - cipalmente comercial en detrimento de la atención respetuosa al paciente. En el presente artículo se revisa cuál ha sido considerada la esencia de la profesión a lo largo de los siglos, se contrasta con la situación actual y se invita a recuperar el ejercicio médico centrado en el paciente

Competencias profesionales y empleo en el futuro periodista: el caso de los estudiantes de periodismo de la Universidad San Pablo CEU

Directory of Open Access Journals (Sweden)

Javier Sierra Sánchez

2012-04-01

Full Text Available La Universidad española ha sufrido una gran transformación desde su adaptación al EEES. El Plan Bolonia propone un replanteamiento de la misión que debe cumplir la Universidad acercando dicha institución a los intereses y necesidades de la empresa y de la sociedad. Uno de los pilares básicos de estos cambios es el que hace referencia a la formación del alumno por competencias. Presentamos un trabajo de investigación científica enfocado en dos aspectos: la adquisición de competencias profesionales en los alumnos de la licenciatura de Periodismo de la Universidad San Pablo CEU y  la importancia que otorgan a diferentes aspectos de un empleo. Éste último aspecto, partiendo del Informe Réflex de ANECA “El profesional flexible en la Sociedad del Conocimiento” establecemos una comparativa entre los resultados allí obtenidos y el caso particular de los alumnos de Periodismo de la San Pablo CEU. Para descubrir estos aspectos presentamos un trabajo empírico para conocer en qué medida los alumnos de último curso de la licenciatura (que no de grado de la Universidad San Pablo CEU han adquirido las competencias profesionales que se reflejan en el Libro Blanco de ANECA de los títulos de grado en Comunicación.Este estudio que presentamos nace con vocación de continuidad en un doble sentido: ser un indicador de la calidad de las enseñanzas que reciben los alumnos de Periodismo de la San Pablo CEU y conocer en un futuro próximo (cuando salga la primera promoción de graduados si existe una diferencia significativa entre los alumnos que cursaron la licenciatura y los nuevos graduados fruto de la reforma universitaria. De este modo, podremos conocer hasta qué punto y qué grado se ha acometido en la Universidad San Pablo CEU esa transformación que conlleva el Plan Bolonia en su sentido más profundo de la reforma.

Synthesis of nano-hydroxyapatite (nHA) from waste mussel shells using a rapid microwave method

Energy Technology Data Exchange (ETDEWEB)

Shavandi, Amin, E-mail: amin.shavandi@postgrad.otago.ac.nz [Department of Food Sciences, University of Otago, Dunedin (New Zealand); Bekhit, Alaa El-Din A. [Department of Food Sciences, University of Otago, Dunedin (New Zealand); Ali, Azam [Department of Applied Sciences, University of Otago, Dunedin (New Zealand); Sun, Zhifa [Department of Physics, University of Otago, Dunedin (New Zealand)

2015-01-15

Nano-crystalline hydroxyapatite (HA, Ca{sub 10}(PO{sub 4}){sub 6}(OH){sub 2}) was produced from waste mussel shells using a rapid microwave irradiation method. Mussel shells were converted to rod like nano-crystalline HA particles of 30–70 nm long using 0.1 M EDTA as a chelating agent for 30 min after an appropriate pre-treatment and an irradiation step in a microwave with a power of 1.1 kW. The produced HA was characterised using X-ray diffraction (XRD), scanning electron microscopy (SEM) coupled with energy-dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), thermo gravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR) and inductively coupled plasma mass spectrometry (ICP-MS) to determine the morphology, particle size, crystal phases, elemental composition and thermal behaviour. Furthermore, to benchmark the synthesized HA obtained from mussel shells, it was compared with a commercially pure HA (Sigma–Aldrich). The thermal analysis showed that the synthesized HA has remarkable heat stability at 1000 °C, and the XRD and FTIR results showed a high purity of the synthesized HA powders. Compared to the conventional hydrothermal treatment, microwave-assisted method has the advantages of an increased rate of HA formation. The obtained HA have potential engineering applications as materials for bone-tissues. - Highlights: • Waste mussel shells were successfully converted to nano sized hydroxyapatite. • Microwave-assisted technique accelerated the conversion process. • The physicochemical properties of the produced hydroxyapatite are reported. • The prepared hydroxyapatite has nano sized particles of less than 100 nm.

Evaluation of nanohydroxyapaptite (nano-HA) coated epigallocatechin-3-gallate (EGCG) cross-linked collagen membranes.

Science.gov (United States)

Chu, Chenyu; Deng, Jia; Man, Yi; Qu, Yili

2017-09-01

Collagen is the main component of extracellular matrix (ECM) with desirable biological activities and low antigenicity. Collagen materials have been widely utilized in guided bone regeneration (GBR) surgery due to its abilities to maintain space for hard tissue growth. However, pure collagen lacks optimal mechanical properties. In our previous study, epigallocatechin-3-gallate (EGCG) cross-linked collagen membranes, with better biological activities and enhanced mechanical properties, may promote osteoblast proliferation, but their effect on osteoblast differentiation is not very significant. Nanohydroxyapatite (nano-HA) is the main component of mineral bone, which possesses exceptional bioactivity properties including good biocompatibility, high osteoconductivity and osteoinductivity, non-immunogenicity and non-inflammatory behavior. Herein, by analyzing the physical and chemical properties as well as the effects on promoting bone regeneration, we have attempted to present a novel EGCG-modified collagen membrane with nano-HA coating, and have found evidence that the novel collagen membrane may promote bone regeneration with a better surface morphology, without destroying collagen backbone. To evaluate the surface morphologies, chemical and mechanical properties of pure collagen membranes, epigallocatechin-3-gallate (EGCG) cross-linked collagen membranes, nano-HA coated collagen membranes, nano-HA coated EGCG-collagen membranes, (ii) to evaluate the bone regeneration promoted by theses membranes. In the present study, collagen membranes were divided into 4 groups: (1) untreated collagen membranes (2) EGCG cross-linked collagen membranes (3) nano-HA modified collagen membranes (4) nano-HA modified EGCG-collagen membranes. Scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) were used to evaluate surface morphologies and chemical properties, respectively. Mechanical properties were determined by differential scanning calorimeter (DSC

Gerrit Rietveld. Casas después de la Schroeder

Directory of Open Access Journals (Sweden)

Rafael García

1997-07-01

Full Text Available Las historias de la arquitectura moderna han incidido una y otra vez en un reducido número de obras que por su insistente atención, han llegado a convertirse en hitos imprescindibles. Entre estos edificios canónicos y prácticamente obligados, está la célebre casa Schröder-Schräder (1924' del arquitecto holandés Gerrit Rietveld. Ampliamente conocida por el público interesado, su gran popularidad ha tenido sin embargo, el efecto de dejar en segundo plano el resto de su obra arquitectónica. A ello se debe añadir además, el hecho de que su trayectoria está fundamentalmente asociada al movimiento neoplasticista, lo que ha tenido por resultado desviar la atención de una obra posterior, más difícilmente clasificable. Sin embargo, ello no significa que dicha obra sea en absoluto carente de atractivo.

Diseño e implementación de cursos abiertos masivos en línea (MOOC: expectativas y consideraciones prácticas

Directory of Open Access Journals (Sweden)

Carmen M. Méndez García

2013-01-01

Full Text Available La popularidad de los cursos abiertos masivos en línea (MOOC durante los dos últimos años ha llevado a muchos diseñadores, docentes e instituciones a interesarse por este nuevo modelo de docencia, que incluso ha llegado a postularse como solución a algunos problemas de la educación universitaria. En este artículo trataremos de analizar cuáles son sus ventajas y desventajas, y sus diferencias respecto a otros modelos de enseñanza (cursos tradicionales, LMS para evaluar su utilidad y viabilidad dentro de la educación superior. También estudiaremos varios cursos creados según este modelo MOOC (específicamente, xMOOC, atendiendo a aspectos de diseño y de expectativas por parte de los distintos agentes del proceso educativo, para sistematizar una serie de consideraciones prácticas que pueden ayudar a quien se acerque al diseño e implementación de MOOC por primera vez.

DESARROLLO TURÍSTICO POR Y PARA LA COMUNIDAD: EL CASO DE LA REPÚBLICA DOMINICANA

Directory of Open Access Journals (Sweden)

María Ángeles Tenor Peña

2013-06-01

Full Text Available El mercado turístico internacional persigue satisfacer las preferencias de un turista que cada vez más busca experimentar el destino con comunidad incluida. En este sentido, la República Dominicana es un interesante caso de estudio para analizar la evolución que el turismo comunitario, desarrollado por y para la comunidad, está teniendo en un país en el que la actividad turística es uno de los pilares de su economía. Se ha analizado cómo la República Dominicana se está adaptando a las tendencias del mercado y cuáles son las repercusiones en el crecimiento y desarrollo de la comunidad. Para ello ha sido un claro referente la publicación de Villareal, R. y Van der Horst, A. (2008, y han resultado clave para las conclusiones a las que se han llegado los datos obtenidos a partir del trabajo de campo realizado.

Reflexiones sobre el psicoanálisis en el umbral del siglo XXI

Directory of Open Access Journals (Sweden)

Eduardo Laverde-Rubio

1993-07-01

Full Text Available ¿En que momento fue creado el psicoanálisis? ¿Cuando Freud usa el termino "psicoanálisis" por primera vez en 1897? ¿Cuando gracias a su autoanálisis reconoce la importancia de la sexualidad infantil y la existencia del complejo de Edipo? ¿O en 1900 cuando publico su primer gran escrito psicoanalítico "La Interpretación de los Sueños"? Pienso que se trato de un desarrollo sutil y no podemos precisar un día de nacimiento; en todo caso es una creación que va de la mana con el siglo XX, sin que hasta ahora se haya producido su deceso, tan anunciado por parte de sus detractores. Todo lo contrario, no solamente se ha afirmado y desarrollado en su aspecto técnico como procedimiento eficaz, para tratamiento de desordenes psicológicos, sino que ha llegado a formar parte del pensamiento y visión del mundo de la cultura occidental.

Restauración, reciclaje y ¿por qué no rehabilitación o reutilización?

Directory of Open Access Journals (Sweden)

Alberto Cedeño Valdiviezo

2009-08-01

Full Text Available La incursión de la relativa nueva disciplina llamada rehabilitación desde fines de los años sesentas en Italia, que viene a sustituir a la tradicional restauración cuando de intervenir el patrimonio urbano se trata, ha traído consigo la inclusión de nuevos conceptos y nuevas tecnologías de construcción, con los consecuentes problemas que esto representa. Este artículo busca aclarar esas dudas encontrando el origen de tales palabras. El uso de la palabra reciclaje deberá hacerse con mesura, en virtud de las connotaciones políticas que tiene. También como consecuencia, los conceptos patrimonio urbano y patrimonio edificado sustituyen al tradicional de centro histórico. Igualmente es objetivo de este trabajo el aclarar como se da este cambio desde la intervención en Bolonia.

PCL-HA microscaffolds for in vitro modular bone tissue engineering.

Science.gov (United States)

Totaro, Alessandra; Salerno, Aurelio; Imparato, Giorgia; Domingo, Concepción; Urciuolo, Francesco; Netti, Paolo Antonio

2017-06-01

The evolution of microscaffolds and bone-bioactive surfaces is a pivotal point in modular bone tissue engineering. In this study, the design and fabrication of porous polycaprolactone (PCL) microscaffolds functionalized with hydroxyapatite (HA) nanoparticles by means of a bio-safe and versatile thermally-induced phase separation process is reported. The ability of the as-prepared nanocomposite microscaffolds to support the adhesion, growth and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in standard and osteogenic media and using dynamic seeding/culture conditions was investigated. The obtained results demonstrated that the PCL-HA nanocomposite microparticles had an enhanced interaction with hMSCs and induced their osteogenic differentiation, even without the exogenous addition of osteogenic factors. In particular, calcium deposition, alizarin red assay, histological analysis, osteogenic gene expression and collagen I secretion were assessed. The results of these tests demonstrated the formation of bone microtissue precursors after 28 days of dynamic culture. These findings suggest that PCL-HA nanocomposite microparticles represent an excellent platform for in vitro modular bone tissue engineering. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin

Energy Technology Data Exchange (ETDEWEB)

Nakamura, Toshio; Tonozuka, Takashi; Kotani, Mao; Obata, Kanae [Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509 (Japan); Oguma, Keiji [Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558 (Japan); Nishikawa, Atsushi, E-mail: nishikaw@cc.tuat.ac.jp [Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509 (Japan); CREST, Japan Science and Technology Agency (Japan)

2007-12-01

HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized. HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.6 Å resolution and the crystal belonged to the hexagonal space group P6{sub 3}. Matthews coefficient and self-rotation function calculations indicate that there is probably one molecule of HA3 in the asymmetric unit. A search for heavy-atom derivatives has been undertaken.

ALP gene expression in cDNA samples from bone tissue engineering using a HA/TCP/Chitosan scaffold

Science.gov (United States)

Stephanie, N.; Katarina, H.; Amir, L. R.; Gunawan, H. A.

2017-08-01

This study examined the potential use of hydroxyapatite (HA)/tricalcium phosphate (TCP)/Chitosan as a bone tissue engineering scaffold. The potential for using HA/TCP/chitosan as a scaffold was analyzed by measuring expression of the ALP osteogenic gene in cDNA from bone biopsies from four Macaque nemestrina. Experimental conditions included control (untreated), treatment with HA/TCP 70:30, HA/TCP 50:50, and HA/TCP/chitosan. cDNA samples were measured quantitively with Real-Time PCR (qPCR) and semi-quantitively by gel electrophoresis. There were no significant differences in ALP gene expression between treatment subjects after two weeks, but the HA/TCP/chitosan treatment gave the highest level of expression after four weeks. The scaffold using the HA/TCP/chitosan combination induced a higher level of expression of the osteogenic gene ALP than did scaffold without chitosan.

Oxidative Stress Posttranslationally Regulates the Expression of Ha-Ras and Ki-Ras in Cultured Astrocytes

Directory of Open Access Journals (Sweden)

Samantha Messina

2012-01-01

Full Text Available Addition of hydrogen peroxide to cultured astrocytes induced a rapid and transient increase in the expression of Ha-Ras and Ki-Ras. Pull-down experiments with the GTP-Ras-binding domain of Raf-1 showed that oxidative stress substantially increased the activation of Ha-Ras, whereas a putative farnesylated activated form of Ki-Ras was only slightly increased. The increase in both Ha-Ras and Ki-Ras was insensitive to the protein synthesis inhibitor, cycloheximide, and was occluded by the proteasomal inhibitor, MG-132. In addition, exposure to hydrogen peroxide reduced the levels of ubiquitinated Ras protein, indicating that oxidative stress leads to a reduced degradation of both isoforms through the ubiquitin/proteasome pathway. Indeed, the late reduction in Ha-Ras and Ki-Ras was due to a recovery of proteasomal degradation because it was sensitive to MG-132. The late reduction of Ha-Ras levels was abrogated by compound PD98059, which inhibits the MAP kinase pathway, whereas the late reduction of Ki-Ras was unaffected by PD98059. We conclude that oxidative stress differentially regulates the expression of Ha-Ras and Ki-Ras in cultured astrocytes, and that activation of the MAP kinase pathway by oxidative stress itself or by additional factors may act as a fail-safe mechanism limiting a sustained expression of the potentially detrimental Ha-Ras.

Control biológico en el cultivo del arroz en Cuba. (Resultados 1970 – 2005.

Directory of Open Access Journals (Sweden)

Rafael Meneses

2007-12-01

Full Text Available El cultivo del arroz en Cuba está afectado por diversas plagas, fundamentalmente: Tagosodes orizicolus, Lissorhoptrus brevirostris, Oebalus insularis y Spodoptera frugiperda. A partir de 1970 se iniciaron los estudios sobre los principales controles biológicos de estas plagas. Para T. orizicolus se ha determinado que Paranagrus perforator y Tytthus parviceps son los principales enemigos naturales de esta plaga. El porcentaje de huevos de T. orizicolus parasitados por P. perforator ha llegado al 50.7% en la etapa de germinación a ahijamiento activo de la planta de arroz. Además, a esta plaga se le ha detectado control por los parasitoides Elenchus sp. y Gonatopus sp. L. brevirostris ha sido manejado en los arrozales con los hongos Metarhizium anisopliae y Beauveria bassiana, obteniendo muy buenos resultados, tanto en el control como en las afectaciones ocasionadas por este insecto. La aplicación de M. anisopliae cepa Niña Bonita resulta satisfactorio para el control de O. insularis. Se ha determinado diversos depredadores, parasitoides y hongos que en determinadas épocas del año ejercer buen control de S. frugiperda, destacándose dentro de ellos: Coleomegilla cubensis, Chelonus texanus y Telenomus spp. C. texanus ha presentado un nivel de parasitismo superior al 80.0 % y Telenomus sp. de 93 %, en condiciones de producción arrocera. Con la presencia de arañas en los arrozales se logra disminuir considerablemente las poblaciones de insectos plagas.

Población flotante: Tipología de visitantes en Tijuana

Directory of Open Access Journals (Sweden)

Nora L. Bringas Rábago

1992-01-01

Full Text Available Desde los inicios del presente siglo, el desarrollo de Tijuana ha sido marcado por el turismo nacional y extranjero. El objetivo de este trabajo es establecer una tipología de los visitantes que llegan a esta ciudad, a partir de los servicios que demandan, enfocando la atención en los turistas extranjeros y los migrantes en sus diferentes modalidades.Se hará referencia específica a los migrantes que han llegado a esta frontera con la esperanza de emigrar hacia Estados Unidos, y que se ven en la necesidad de permanecer en la ciudad y hacer uso de diversos servicios, principalmente hospedaje y alimentación

Un siglo aún por descubrir en la Historia de España: el siglo V después de Cristo

Directory of Open Access Journals (Sweden)

Francisco Javier Guzmán Armario

2016-06-01

Full Text Available El siglo V después de Cristo es, sin duda, el más desconocido de la Historia de España. Ello se debe a que, desde fines del siglo XIX, los historiadores han cultivado una imagen de esa centuria marcada por los vicios de la historiografía tradicional que incluso ha llegado a nuestro tiempo. En la actualidad, es necesario recurrir a nuevos planteamientos metodológicos (en este artículo el autor propone cuatro argumentos al respecto y a nuevas perspectivas en Arqueología si se quiere corregir esa deficiencia en un siglo tan importante para la Historia de España.

Una minga para el posdesarrollo

Directory of Open Access Journals (Sweden)

Arturo Escobar

2011-01-01

Full Text Available El artículo sintetiza algunas conclusiones a las que han llegado movimientos sociales y académicos en las últimas dos décadas al reflexionar sobre la globalización y sus implicaciones; el modelo dominante, basado en las nociones de "desarrollo" y "modernidad", ha entrado en crisis. Por lo tanto, se hace necesario avanzar en una transición cultural que se pueda comprender como un cambio de época y no sólo como una época de cambios. Para llevar a cabo esta transformación, se deben resolver las con-tradicciones existentes entre el neodesarrollismo y el postdesarrollo, por supuesto, a favor de éste último.

Prótesis total y unicompartimental en el manejo de artrosis grave de rodilla

Directory of Open Access Journals (Sweden)

DR. U. Jaime duboy

2014-09-01

Full Text Available La artrosis grave de rodilla es una patología prevalente y puede ser en algunos casos invalidante. Se revisan los fundamentos e historia sobre cómo la prótesis de rodilla ha llegado a ser el tratamiento de elección para eliminar el dolor y recuperar función, mejorando la calidad de vida de los pacientes. Se muestran las alternativas actuales que incluyen las prótesis Unicompartamentales, de creciente uso en los últimos años y se señalan también las mejoras en los cuidados perioperatorios y rehabilitación que han permitido una mejora significativa de los resultados.

Perniola, Mario: La estética contemporánea y El arte expandido.

Directory of Open Access Journals (Sweden)

Óscar Ortega Ruiz

2016-01-01

Full Text Available Recientemente, han llegado a las librerías dos nuevas publicaciones de una de las grandes personalidades del pensamiento filosófico italiano, el profesor Mario Perniola, catedrático de Estética de la Universidad Tor Vergata de Roma. Este veterano intelectual de origen piamontés, nacido en 1941, ha ddedicado la mayor parte de su carrera a la reflexión acerca de los problemas y retos surgidos en el seno de la disciplina estética de los últimos decenios, tales como la evolución de las vanguardias, el situacionismo –movimiento en el que estuvo directamente involucrado–, los estudios culturales, el postmodernismo, el posthumanismo, etc.

The use of acetone to enhance the infiltration of HA nanoparticles into a demineralized dentin collagen matrix.

Science.gov (United States)

Besinis, Alexandros; van Noort, Richard; Martin, Nicolas

2016-03-01

This study investigates the role of acetone, as a carrier for nano-hydroxyapatite (nano-HA) in solution, to enhance the infiltration of fully demineralized dentin with HA nanoparticles (NPs). Dentin specimens were fully demineralized and subsequently infiltrated with two types of water-based nano-HA solutions (one containing acetone and one without). Characterization of the dentin surfaces and nano-HA particles was performed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The surface wettability and infiltration capacity of the nano-HA solutions were quantified by means of contact angle measurements and energy dispersive X-ray spectroscopy (EDS), respectively. Contact angle measurements were taken at baseline and repeated at regular intervals to assess the effect of acetone. The P and Ca levels of infiltrated dentin specimens were measured and compared to sound dentin and non-infiltrated controls. The presence of acetone resulted in an eight-fold decrease in the contact angles of the nano-HA solutions recorded on the surface of demineralized dentin compared to nano-HA solutions without acetone (one-way ANOVA, pacetone. Infiltration of demineralized dentin with the nano-HA solution containing acetone restored the lost mineral content by 50%, whereas the mean mineralization values for P and Ca in dentin treated with the acetone-free nano-HA solution were less than 6%. Acetone was shown to act as a vehicle to enhance the capacity to infiltrate demineralized dentin with HA NPs. The successful infiltration of dentin collagen with HA NPs provides a suitable scaffold, whereby the infiltrated HA NPs have the potential to act as seeds that may initiate heterogenous mineral growth when exposed to an appropriate mineral-rich environment. Copyright © 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

Ha-DEF1, a sunflower defensin, induces cell death in Orobanche parasitic plants.

Science.gov (United States)

de Zélicourt, Axel; Letousey, Patricia; Thoiron, Séverine; Campion, Claire; Simoneau, Philippe; Elmorjani, Khalil; Marion, Didier; Simier, Philippe; Delavault, Philippe

2007-08-01

Plant defensins are small basic peptides of 5-10 kDa and most of them exhibit antifungal activity. In a sunflower resistant to broomrape, among the three defensin encoding cDNA identified, SF18, SD2 and HaDef1, only HaDef1 presented a preferential root expression pattern and was induced upon infection by the root parasitic plant Orobanche cumana. The amino acid sequence deduced from HaDef1 coding sequence was composed of an endoplasmic reticulum signal sequence of 28 amino acids, a standard defensin domain of 50 amino-acid residues and an unusual C-terminal domain of 30 amino acids with a net positive charge. A 5.8 kDa recombinant mature Ha-DEF1 corresponding to the defensin domain was produced in Escherichia coli and was purified by means of a two-step chromatography procedure, Immobilized Metal Affinity Chromatography (IMAC) and Ion Exchange Chromatography. Investigation of in vitro antifungal activity of Ha-DEF1 showed a strong inhibition on Saccharomyces cerevisiae growth linked to a membrane permeabilization, and a morphogenetic activity on Alternaria brassicicola germ tube development, as already reported for some other plant defensins. Bioassays also revealed that Ha-DEF1 rapidly induced browning symptoms at the radicle apex of Orobanche seedlings but not of another parasitic plant, Striga hermonthica, nor of Arabidopsis thaliana. FDA vital staining showed that these browning areas corresponded to dead cells. These results demonstrate for the first time a lethal effect of defensins on plant cells. The potent mode of action of defensin in Orobanche cell death and the possible involvement in sunflower resistance are discussed.

Designing, preparing and evaluation of novel HA/Ti composite coating for endodontic dental implant

Directory of Open Access Journals (Sweden)

Fathi MH.

2002-08-01

Full Text Available Nowadays, application of implants as a new method for replacing extracted teeth have been improved. So, many researches have been performed for improving the characteristics of implants. The aim of this study was to design and produce a desired coating in order to obtaining two goals including; improvement of the corrosion behavior of metallic endodontic implant and the bone osseointegration simultaneously. Stainless steel 316L (SS, cobalt-chromium alloy (Vit and commercial pure titanium (cpTi were chosen as metallic substrates and hydroxyapatite coating (HAC were performed by plasma-spraying (PS process on three different substrates. A novel double layer Hydroxyapatite/Titanium (HA/Ti composite coating composed of a HA top layer and a Ti under layer was prepared using PS and physical vapor deposition (PVD process respectively on SS. Structural characterization techniques including XRD, SEM and EDX were utilized to investigate the microstructure, morpholgy and crystallinity of the coatings. Electrochemical potentiodynamic tests were performed in physiological solutions in order to determine and compare the corrosion behavior of the coated and uncoated specimens behavior as an indication of biocmpatibility. Results indicated that the cpTi possesses the highest and SS the lowest corrosion resistance (highest corrosion current density between uncoated substrates. This trend was independent to the type of physiological environment. The HA coating decreased the corrosion current density of HA coated metallic implants but did not change that trend. HAC acted as a mechanical barrier on the metallic substrate but could not prevent the interaction between metallic substrate and environment completely. The HA/Ti composite coating improved the corrosion behavior of SS. The corrosion current density of HA/Ti coated SS decreased and was exactly similar to single HA coated cpTi in physiological solutions. The results indicated that HA/Ti composite coated SS

Sequence diversity within the HA-1 gene as detected by melting temperature assay without oligonucleotide probes

Directory of Open Access Journals (Sweden)

Mattiuz Pier

2005-10-01

Full Text Available Abstract Background The minor histocompatibility antigens (mHags are self-peptides derived from common cellular proteins and presented by MHC class I and II molecules. Disparities in mHags are a potential risk for the development of graft-versus-host disease (GvHD in the recipients of bone marrow from HLA-identical donors. Two alleles have been identified in the mHag HA-1. The correlation between mismatches of the mHag HA-1 and GvHD has been suggested and methods to facilitate large-scale testing were afterwards developed. Methods We used sequence specific primer (SSP PCR and direct sequencing to detect HA-1 gene polymorphisms in a sample of 131 unrelated Italian subjects. We then set up a novel melting temperature (Tm assay that may help identification of HA-1 alleles without oligonucleotide probes. Results We report the frequencies of HA-1 alleles in the Italian population and the presence of an intronic 5 base-pair deletion associated with the immunogeneic allele HA-1H. We also detected novel variable sites with respect to the consensus sequence of HA-1 locus. Even though recombination/gene conversion events are documented, there is considerable linkage disequilibrium in the data. The gametic associations between HA-1R/H alleles and the intronic 5-bp ins/del polymorphism prompted us to try the Tm analysis with SYBR® Green I. We show that the addition of dimethylsulfoxide (DMSO during the assay yields distinct patterns when amplicons from HA-1H homozygotes, HA-1R homozygotes, and heterozygotes are analysed. Conclusion The possibility to use SYBR® Green I to detect Tm differences between allelic variants is attractive but requires great caution. We succeeded in allele discrimination of the HA-1 locus using a relatively short (101 bp amplicon, only in the presence of DMSO. We believe that, at least in certain assets, Tm assays may benefit by the addition of DMSO or other agents affecting DNA strand conformation and stability.

Microstructure and mechanical properties of plasma sprayed HA/YSZ/Ti-6Al-4V composite coatings.

Science.gov (United States)

Khor, K A; Gu, Y W; Pan, D; Cheang, P

2004-08-01

Plasma sprayed hydroxyapatite (HA) coatings on titanium alloy substrate have been used extensively due to their excellent biocompatibility and osteoconductivity. However, the erratic bond strength between HA and Ti alloy has raised concern over the long-term reliability of the implant. In this paper, HA/yttria stabilized zirconia (YSZ)/Ti-6Al-4V composite coatings that possess superior mechanical properties to conventional plasma sprayed HA coatings were developed. Ti-6Al-4V powders coated with fine YSZ and HA particles were prepared through a unique ceramic slurry mixing method. The so-formed composite powder was employed as feedstock for plasma spraying of the HA/YSZ/Ti-6Al-4V coatings. The influence of net plasma energy, plasma spray standoff distance, and post-spray heat treatment on microstructure, phase composition and mechanical properties were investigated. Results showed that coatings prepared with the optimum plasma sprayed condition showed a well-defined splat structure. HA/YSZ/Ti-6Al-4V solid solution was formed during plasma spraying which was beneficial for the improvement of mechanical properties. There was no evidence of Ti oxidation from the successful processing of YSZ and HA coated Ti-6Al-4V composite powders. Small amount of CaO apart from HA, ZrO(2) and Ti was present in the composite coatings. The microhardness, Young's modulus, fracture toughness, and bond strength increased significantly with the addition of YSZ. Post-spray heat treatment at 600 degrees C and 700 degrees C for up to 12h was found to further improve the mechanical properties of coatings. After the post-spray heat treatment, 17.6% increment in Young's modulus (E) and 16.3% increment in Vicker's hardness were achieved. The strengthening mechanisms of HA/YSZ/Ti-6Al-4V composite coatings were related to the dispersion strengthening by homogeneous distribution of YSZ particles in the matrix, the good mechanical properties of Ti-6Al-4V and the formation of solid solution among HA

Functionalized PCL/HA nanocomposites as microporous membranes for bone regeneration

Energy Technology Data Exchange (ETDEWEB)

Basile, Maria Assunta; Gomez d' Ayala, Giovanna; Malinconico, Mario [Institute for Polymers, Composites and Biomaterials, CNR, Via Campi Flegrei 34, Pozzuoli (Naples) (Italy); Laurienzo, Paola, E-mail: paola.laurienzo@ipcb.cnr.it [Institute for Polymers, Composites and Biomaterials, CNR, Via Campi Flegrei 34, Pozzuoli (Naples) (Italy); Coudane, Jean; Nottelet, Benjamin [Institut des Biomolécules Max Mousseron (IBMM), Artificial Biopolymers Group, CNRS UMR 5247, University of Montpellier 1, Faculty of Pharmacy, 15 Av. C. Flahault, Montpellier 34093 (France); Ragione, Fulvio Della [Department of Biochemistry and Biophysics, Second University of Naples, Via L. De Crecchio 7, Naples (Italy); Oliva, Adriana, E-mail: adriana.oliva@unina2.it [Department of Biochemistry and Biophysics, Second University of Naples, Via L. De Crecchio 7, Naples (Italy)

2015-03-01

In the present work, microporous membranes based on poly(ε-caprolactone) (PCL) and PCL functionalized with amine (PCL-DMAEA) or anhydride groups (PCL-MAGMA) were realized by solvent–non solvent phase inversion and proposed for use in Guided Tissue Regeneration (GTR). Nanowhiskers of hydroxyapatite (HA) were also incorporated in the polymer matrix to realize nanocomposite membranes. Scanning Electron Microscopy (SEM) showed improved interfacial adhesion with HA for functionalized polymers, and highlighted substantial differences in the porosity. A relationship between the developed porous structure of the membrane and the chemical nature of grafted groups was proposed. Compared to virgin PCL, hydrophilicity increases for functionalized PCL, while the addition of HA influences significantly the hydrophilic characteristics only in the case of virgin polymer. A significant increase of in vitro degradation rate was found for PCL-MAGMA based membranes, and at lower extent of PCL-DMAEA membranes. The novel materials were investigated regarding their potential as support for cell growth in bone repair using multipotent mesenchymal stromal cells (MSC) as a model. MSC plated onto the various membranes were analyzed in terms of adhesion, proliferation and osteogenic capacity that resulted to be related to chemical as well as porous structure. In particular, PCL-DMAEA and the relative nanocomposite membranes are the most promising in terms of cell-biomaterial interactions. - Graphical abstract: Functionalized PCL is used to realize nanocomposites with hydroxyapatite (HA) in the form of microporous membranes. The influence of different grafted groups on mechanical properties, in vitro degradation, porous membrane structure and interaction with mesenchymal stromal cells (MSC) is discussed. - Highlights: • Functionalized PCL shows faster in vitro degradation rate. • Functionalized PCL shows superior cell adhesion, proliferation and differentiation. • Nanocomposites based

Functionalized PCL/HA nanocomposites as microporous membranes for bone regeneration

International Nuclear Information System (INIS)

Basile, Maria Assunta; Gomez d'Ayala, Giovanna; Malinconico, Mario; Laurienzo, Paola; Coudane, Jean; Nottelet, Benjamin; Ragione, Fulvio Della; Oliva, Adriana

2015-01-01

In the present work, microporous membranes based on poly(ε-caprolactone) (PCL) and PCL functionalized with amine (PCL-DMAEA) or anhydride groups (PCL-MAGMA) were realized by solvent–non solvent phase inversion and proposed for use in Guided Tissue Regeneration (GTR). Nanowhiskers of hydroxyapatite (HA) were also incorporated in the polymer matrix to realize nanocomposite membranes. Scanning Electron Microscopy (SEM) showed improved interfacial adhesion with HA for functionalized polymers, and highlighted substantial differences in the porosity. A relationship between the developed porous structure of the membrane and the chemical nature of grafted groups was proposed. Compared to virgin PCL, hydrophilicity increases for functionalized PCL, while the addition of HA influences significantly the hydrophilic characteristics only in the case of virgin polymer. A significant increase of in vitro degradation rate was found for PCL-MAGMA based membranes, and at lower extent of PCL-DMAEA membranes. The novel materials were investigated regarding their potential as support for cell growth in bone repair using multipotent mesenchymal stromal cells (MSC) as a model. MSC plated onto the various membranes were analyzed in terms of adhesion, proliferation and osteogenic capacity that resulted to be related to chemical as well as porous structure. In particular, PCL-DMAEA and the relative nanocomposite membranes are the most promising in terms of cell-biomaterial interactions. - Graphical abstract: Functionalized PCL is used to realize nanocomposites with hydroxyapatite (HA) in the form of microporous membranes. The influence of different grafted groups on mechanical properties, in vitro degradation, porous membrane structure and interaction with mesenchymal stromal cells (MSC) is discussed. - Highlights: • Functionalized PCL shows faster in vitro degradation rate. • Functionalized PCL shows superior cell adhesion, proliferation and differentiation. • Nanocomposites based

Avoimen lähdekoodin HA-tietokannat

OpenAIRE

Vartio, Sami

2014-01-01

Insinöörityössä perehdyttiin avoimen lähdekoodin periaatteisiin ja lisenssimalleihin sekä huomioitiin liiketaloudelliset lähtökohdat. Tavoitteena oli tutustua järjestelmän saatavuuteen sekä klusteroinnin periaatteisiin. Järjestelmän saatavuus (HA) on tietojärjestelmien suunnittelussa käytettävä käytäntö, joka pyrkii siihen että järjestelmä on aina käyttäjän käytettävissä. Aluksi käsiteltiin perustietoja sekä teoriaa. Tutkimuksen edetessä paneuduttiin laajemmin yleisimpiin avoimen lähdeko...

Clinical significance of determination of serum HA, PCIII and ADA levels in patients with chronic hepatitis C

International Nuclear Information System (INIS)

Zhang Jing; Yu Fan; Zhao Changxin

2006-01-01

Objective: To explore the changes of serum HA, PCIII and ADA levels in patients with chronic hepatitis C. Methods: Serum HA, PCIII and ADA levels were detected with RIA in 42 cases with chronic hepatitis C and 35 controls. Results: The levels of serum HA, PC III and ADA in the patients were significantly higher than those in controls (P<0.01). Serum HA, PC m and ADA levels were positive correlated (r=0.6178, 0.6211, P<0.01). Conclusion: The results suggested the serum HA, PC m and ADA levels can reflect the degree of hepatic injury in the patients with cirrhosis, detection of serum HA, PC m and ADA levels is helpful in estimating the status of the disease. (authors)

Vaccine-induced anti-HA2 antibodies promote virus fusion and enhance influenza virus respiratory disease.

Science.gov (United States)

Khurana, Surender; Loving, Crystal L; Manischewitz, Jody; King, Lisa R; Gauger, Phillip C; Henningson, Jamie; Vincent, Amy L; Golding, Hana

2013-08-28

Vaccine-induced disease enhancement has been described in connection with several viral vaccines in animal models and in humans. We investigated a swine model to evaluate mismatched influenza vaccine-associated enhanced respiratory disease (VAERD) after pH1N1 infection. Vaccinating pigs with whole inactivated H1N2 (human-like) virus vaccine (WIV-H1N2) resulted in enhanced pneumonia and disease after pH1N1 infection. WIV-H1N2 immune sera contained high titers of cross-reactive anti-pH1N1 hemagglutinin (HA) antibodies that bound exclusively to the HA2 domain but not to the HA1 globular head. No hemagglutination inhibition titers against pH1N1 (challenge virus) were measured. Epitope mapping using phage display library identified the immunodominant epitope recognized by WIV-H1N2 immune sera as amino acids 32 to 77 of pH1N1-HA2 domain, close to the fusion peptide. These cross-reactive anti-HA2 antibodies enhanced pH1N1 infection of Madin-Darby canine kidney cells by promoting virus membrane fusion activity. The enhanced fusion activity correlated with lung pathology in pigs. This study suggests a role for fusion-enhancing anti-HA2 antibodies in VAERD, in the absence of receptor-blocking virus-neutralizing antibodies. These findings should be considered during the evaluation of universal influenza vaccines designed to elicit HA2 stem-targeting antibodies.

Isothermal Crystallization Kinetics of HDPE/HA Compounds Irradiated with Sterilization Doses of Gamma Rays

International Nuclear Information System (INIS)

Albano, C.

2006-01-01

The objective of this work was to study the isothermal crystallization of High Density Polyethylene/Hydroxyapatite nanocomposites, with 2 and 5 ppc of HA, irradiated with 25 kGy (sterilization dose) of γ-Ray from a 60 C o source, at a rate of 4.8 kGy/h in air and at room temperature. The selected crystallization temperatures were 118, 117, 116 and 115 degree. The crystallization kinetics was analyzed using the Avrami's model whose parameters were optimized using a non-linear regression technique. Regression results show that the Avrami exponent varies between 1.8 and 1.5, meaning that the spherulitic growth is mainly two dimensional. Values for specific crystallization constant 'k' were found to be higher for HDPE/HA compounds than for pure HDPE, clearly indicating the presence of an HA nucleation effect. It was also observed that values for the specific crystallization constant 'k' decreases with increasing temperatures, being this effect more noticeable for HDPE/HA compounds than for pure HDPE. Regarding to irradiated samples, their 'k' values were found to be lower than those for non irradiated samples, the difference getting more significant with decreasing crystallization temperature. Simulation of experimental data with the Avrami's model show a clear influence of the crystallization temperature, the HA content in the sample and the amount of applied radiation. It was also observed that the Avrami model correlates satisfactorily experimental data for not irradiated samples of pure HDPE and HDPE/HA compounds at the highest crystallization (T c ). However, as the crystallization temperature decreases, the values simulated with the Avrami model increasingly deviate from experimental data, specifically at the highest values of the relative crystallinity. This effect is even stronger on irradiated samples of HDPE and HDPE/HA compounds

Development and characterization of poli composites (ether ether ketone)(PEEK)(Hydroxyapatite(HA)

International Nuclear Information System (INIS)

Ferreira, V.P.; Santos, F.S.F.; Sa, M.D. de; Fook, M.V.L.

2016-01-01

The objective of this work was to develop PEEK / HA composites, combining the biological activity of the ceramic phase with the properties of the polymer phase, the materials used in this research were Poly (ether-ether-ketone) (PEEK) and Hydroxyapatite (HA) (50, 60, 70 and 80% m / v HA), this material was subjected to a load of two tons followed by a thermal treatment at 390 ° for a period of 30 minutes. Then they were characterized by FTIR, DRX and MO. In the physical-chemical characterization of FTIR and XRD, it was not possible to identify significant alterations. In the FTIR spectra of the composites, there is no formation of new identifiable chemical bonds. In the composites XRD diffractograms a profile similar to the ceramic phase was observed, with peaks increasing in intensity and narrowing proportional to the increase of the hydroxyapatite concentration in the composites. In optical microscopy it is possible to observe surfaces with heterogeneous morphology, with signs of roughness and in the cross section we observe a heterogeneous aspect, rich in regions with large agglomerates and lighter particles. Considering the processing aspects, the technique proved to be effective for the development of PEEK /HA composites. (author)

Porous HA-Al2O3 composite characterization using corn starch as a porogen agent

International Nuclear Information System (INIS)

Silva, L.A.J. da; Galdino, A.G.S.; Cardoso, G.B.C.; Zavaglia, C.A.C.

2011-01-01

The porous ceramics based on hydroxyapatite have great potential for application in bone grafts due to its chemical similarity with the mineral phase of bone tissue, but have poor biomechanical properties, which cause limitations in its applications. This work aims to analyze the structural characteristics of porous ceramics obtained by addition of hydroxyapatite (HA, sintered in the laboratory), Corn Starch (CS, commercial) and Al 2 O 3 (ALCOA), at different temperatures. Samples were made of dense HA (100% HA), porous (70% HA - 30% CS) and with addition of 2.5%, 5% and 7.5% Al 2 O 3 porous composition. The samples were sintered at 1250°C, 1300 deg C and 135 0°C and characterized by: XRF, XRD, SEM and density by the Archimedes method. Concludes It is the possibility of obtaining samples porous HA / Al 2 O 3 using starch as porogenic agent. The temperature and concentration of Al 2 O 3 most appropriate were: 1250°C and 7.5%. (author)

Study of the gamma irradiation effects on the PMMA/HA and PMMA/SW

Energy Technology Data Exchange (ETDEWEB)

Silva, P., E-mail: silva@ivic.v [Instituto Venezolano de Investigaciones Cientificas, Centro de Fisica, Carretera Panamericana Km. 11, Caracas 1020-A (Venezuela, Bolivarian Republic of); Albano, C. [Universidad Central de Venezuela, Facultad de Ingenieria (Venezuela, Bolivarian Republic of); Instituto Venezolano de Investigaciones Cientificas, Centro de Quimica (Venezuela, Bolivarian Republic of); Perera, R. [Departamento de Mecanica, Universidad Simon Bolivar, Caracas 1080-A (Venezuela, Bolivarian Republic of); Dominguez, N. [Instituto Venezolano de Investigaciones Cientificas, Centro de Quimica (Venezuela, Bolivarian Republic of)

2010-03-15

The behavior of the poly(methyl methacrylate) (PMMA) under the action of gamma radiation has been sufficiently studied. In this work, we present results from melt flow index (MFI), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and electron paramagnetic resonance (EPR) of PMMA composites with hydroxyapatite (HA) and seaweed residues (SW) irradiated with gamma rays at 1.08 kGy/h. Composites of PMMA/HA and PMMA/SW with 10%, 20% and 30% of the filler were prepared. The results show an increase in the MFI values with the integral dose of radiation, being consistent with chain-scission reactions. No EPR signal was observed in pure PMMA, while in the composites, the typical EPR signal of the PMMA radicals was observed, which increased with the amount of HA or SW. When comparing the relative intensities of the EPR signals for both types of composites, a slight increase in the concentration of free radicals generated in the sample with SW respect to that of PMMA/HA composite was obtained. A decay of the total free radical concentration was observed as time elapsed.

Does PEEK/HA Enhance Bone Formation Compared With PEEK in a Sheep Cervical Fusion Model?

Science.gov (United States)

Walsh, William R; Pelletier, Matthew H; Bertollo, Nicky; Christou, Chris; Tan, Chris

2016-11-01

Polyetheretherketone (PEEK) has a wide range of clinical applications but does not directly bond to bone. Bulk incorporation of osteoconductive materials including hydroxyapatite (HA) into the PEEK matrix is a potential solution to address the formation of a fibrous tissue layer between PEEK and bone and has not been tested. Using in vivo ovine animal models, we asked: (1) Does PEEK-HA improve cortical and cancellous bone ongrowth compared with PEEK? (2) Does PEEK-HA improve bone ongrowth and fusion outcome in a more challenging functional ovine cervical fusion model? The in vivo responses of PEEK-HA Enhanced and PEEK-OPTIMA ® Natural were evaluated for bone ongrowth in the form of dowels implanted in the cancellous and cortical bone of adult sheep and examined at 4 and 12 weeks as well as interbody cervical fusion at 6, 12, and 26 weeks. The bone-implant interface was evaluated with radiographic and histologic endpoints for a qualitative assessment of direct bone contact of an intervening fibrous tissue later. Gamma-irradiated cortical allograft cages were evaluated as well. Incorporating HA into the PEEK matrix resulted in more direct bone apposition as opposed to the fibrous tissue interface with PEEK alone in the bone ongrowth as well as interbody cervical fusions. No adverse reactions were found at the implant-bone interface for either material. Radiography and histology revealed resorption and fracture of the allograft devices in vivo. Incorporating HA into PEEK provides a more favorable environment than PEEK alone for bone ongrowth. Cervical fusion was improved with PEEK-HA compared with PEEK alone as well as allograft bone interbody devices. Improving the bone-implant interface with a PEEK device by incorporating HA may improve interbody fusion results and requires further clinical studies.

High Altitude Affects Nocturnal Non-linear Heart Rate Variability: PATCH-HA Study

Directory of Open Access Journals (Sweden)

Christopher J. Boos

2018-04-01

Full Text Available Background: High altitude (HA exposure can lead to changes in resting heart rate variability (HRV, which may be linked to acute mountain sickness (AMS development. Compared with traditional HRV measures, non-linear HRV appears to offer incremental and prognostic data, yet its utility and relationship to AMS have been barely examined at HA. This study sought to examine this relationship at terrestrial HA.Methods: Sixteen healthy British military servicemen were studied at baseline (800 m, first night and over eight consecutive nights, at a sleeping altitude of up to 3600 m. A disposable cardiac patch monitor was used, to record the nocturnal cardiac inter-beat interval data, over 1 h (0200–0300 h, for offline HRV assessment. Non-linear HRV measures included Sample entropy (SampEn, the short (α1, 4–12 beats and long-term (α2, 13–64 beats detrend fluctuation analysis slope and the correlation dimension (D2. The maximal rating of perceived exertion (RPE, during daily exercise, was assessed using the Borg 6–20 RPE scale.Results: All subjects completed the HA exposure. The average age of included subjects was 31.4 ± 8.1 years. HA led to a significant fall in SpO2 and increase in heart rate, LLS and RPE. There were no significant changes in the ECG-derived respiratory rate or in any of the time domain measures of HRV during sleep. The only notable changes in frequency domain measures of HRV were an increase in LF and fall in HFnu power at the highest altitude. Conversely, SampEn, SD1/SD2 and D2 all fell, whereas α1 and α2 increased (p < 0.05. RPE inversely correlated with SD1/SD2 (r = -0.31; p = 0.002, SampEn (r = -0.22; p = 0.03, HFnu (r = -0.27; p = 0.007 and positively correlated with LF (r = 0.24; p = 0.02, LF/HF (r = 0.24; p = 0.02, α1 (r = 0.32; p = 0.002 and α2 (r = 0.21; p = 0.04. AMS occurred in 7/16 subjects (43.8% and was very mild in 85.7% of cases. HRV failed to predict AMS.Conclusion: Non-linear HRV is more sensitive to the

Iglesia en Riola, Italia

Directory of Open Access Journals (Sweden)

Aalto, Alvar

1979-08-01

Full Text Available This church is surrounded by the marvelous landscape — both in summer and winter— of Piola di Vergato, near Bologna, on the River Reno. It was commissioned by Cardinal Lercaro, Archbishop of Bologna and the initial contacts were during 1965. The building is of outstanding beauty and originality, mainly using concrete for the bearing members, copper for the roof. Cerrara marbie for the altar and surrounding areas, local sandstone for outside facings, red Cotto Fiorentino Tuscany clay tile flooring. The church has been built with Italian and Finnish technical assistance from Aarne Hollmen, Claes, Holm, Hamilkar Aalto, Olavi Ebeling, Vezio Nava among others.

Está asentada en el maravilloso paisaje —tanto en invierno como en verano— de Riola de Vergato, cerca de Bolonia, y sobre el cauce del rio Reno. Fue encargada por el cardenal Lercaro, arzobispo de Bolonia en 1965, año en que comenzaron los contactos. En principio el programa completo se componía de iglesia, centro parroquial, campanario, explanada para grandes concentraciones de fieles, residencia de ancianos y jardín de infancia, si bien estos dos últimos fueron suprimidos posteriormente. Aún ahora quedan por terminar muchos detalles de mobiliario y algunos cuerpos de edificación que se espera puedan ser subvencionados y realizados en breve. El edificio es de una gran belleza y originalidad notables, estando construido, fundamentalmente, a base de hormigón en elementos resistentes; cobre en la cubierta; mármol de Carrara en altar y zonas próximas; piedra arenisca de la localidad, en revestimientos exteriores; solados con plaqueta roja de Cotto Florentino Tuscany. Se ha contado con la ayuda y aportación de instalaciones y técnicos finlandeses e italianos, tales como Aarne Hollmen, Claes Holm, Hamilkar Aalto, Olavi Ebeling y Vezio Nava.

Understanding the undelaying mechanism of HA-subtyping in the level of physic-chemical characteristics of protein.

Science.gov (United States)

Ebrahimi, Mansour; Aghagolzadeh, Parisa; Shamabadi, Narges; Tahmasebi, Ahmad; Alsharifi, Mohammed; Adelson, David L; Hemmatzadeh, Farhid; Ebrahimie, Esmaeil

2014-01-01

The evolution of the influenza A virus to increase its host range is a major concern worldwide. Molecular mechanisms of increasing host range are largely unknown. Influenza surface proteins play determining roles in reorganization of host-sialic acid receptors and host range. In an attempt to uncover the physic-chemical attributes which govern HA subtyping, we performed a large scale functional analysis of over 7000 sequences of 16 different HA subtypes. Large number (896) of physic-chemical protein characteristics were calculated for each HA sequence. Then, 10 different attribute weighting algorithms were used to find the key characteristics distinguishing HA subtypes. Furthermore, to discover machine leaning models which can predict HA subtypes, various Decision Tree, Support Vector Machine, Naïve Bayes, and Neural Network models were trained on calculated protein characteristics dataset as well as 10 trimmed datasets generated by attribute weighting algorithms. The prediction accuracies of the machine learning methods were evaluated by 10-fold cross validation. The results highlighted the frequency of Gln (selected by 80% of attribute weighting algorithms), percentage/frequency of Tyr, percentage of Cys, and frequencies of Try and Glu (selected by 70% of attribute weighting algorithms) as the key features that are associated with HA subtyping. Random Forest tree induction algorithm and RBF kernel function of SVM (scaled by grid search) showed high accuracy of 98% in clustering and predicting HA subtypes based on protein attributes. Decision tree models were successful in monitoring the short mutation/reassortment paths by which influenza virus can gain the key protein structure of another HA subtype and increase its host range in a short period of time with less energy consumption. Extracting and mining a large number of amino acid attributes of HA subtypes of influenza A virus through supervised algorithms represent a new avenue for understanding and

Understanding the undelaying mechanism of HA-subtyping in the level of physic-chemical characteristics of protein.

Directory of Open Access Journals (Sweden)

Mansour Ebrahimi

Full Text Available The evolution of the influenza A virus to increase its host range is a major concern worldwide. Molecular mechanisms of increasing host range are largely unknown. Influenza surface proteins play determining roles in reorganization of host-sialic acid receptors and host range. In an attempt to uncover the physic-chemical attributes which govern HA subtyping, we performed a large scale functional analysis of over 7000 sequences of 16 different HA subtypes. Large number (896 of physic-chemical protein characteristics were calculated for each HA sequence. Then, 10 different attribute weighting algorithms were used to find the key characteristics distinguishing HA subtypes. Furthermore, to discover machine leaning models which can predict HA subtypes, various Decision Tree, Support Vector Machine, Naïve Bayes, and Neural Network models were trained on calculated protein characteristics dataset as well as 10 trimmed datasets generated by attribute weighting algorithms. The prediction accuracies of the machine learning methods were evaluated by 10-fold cross validation. The results highlighted the frequency of Gln (selected by 80% of attribute weighting algorithms, percentage/frequency of Tyr, percentage of Cys, and frequencies of Try and Glu (selected by 70% of attribute weighting algorithms as the key features that are associated with HA subtyping. Random Forest tree induction algorithm and RBF kernel function of SVM (scaled by grid search showed high accuracy of 98% in clustering and predicting HA subtypes based on protein attributes. Decision tree models were successful in monitoring the short mutation/reassortment paths by which influenza virus can gain the key protein structure of another HA subtype and increase its host range in a short period of time with less energy consumption. Extracting and mining a large number of amino acid attributes of HA subtypes of influenza A virus through supervised algorithms represent a new avenue for

Consolidation de centres de recherche sur la reconstruction en Haïti ...

International Development Research Centre (IDRC) Digital Library (Canada)

Haïti doit encore relever des défis de taille en ce qui a trait à l'édification de l'État et à la démocratisation. Un groupe de pays latino-américains assume un rôle de premier plan au sein des forces de maintien de la paix coordonnées par la Mission des Nations Unies pour la stabilisation en Haïti (MINUSTAH). L'efficacité de ...

Mechanical properties of titanium-hydroxyapatite (Ti-HA) composite coating on stainless steel prepared by thermal spraying

Science.gov (United States)

Rosmamuhamadani, R.; Azhar, N. H.; Talari, M. K.; Yahaya, Sabrina M.; Sulaiman, S.; Ismail, M. I. S.

2017-09-01

Addition of hydroxyapatite (HA) can enhance the bioactivity of the common metallic implant due to its similarity with natural bones and teeth. In this investigation, high velocity oxy-fuel (HVOFT) technique was used to deposit titanium-hydroxyapatite (Ti-HA) composite on stainless steel substrate plate with different percentage of HA for biomedical applications. The aim of this research is to investigate the mechanical properties of Ti-HA coating such as hardness, adhesion strength and wear behaviour. The hardness and strength was determined by using SHIMADZU-microhardness Vickers tester and PosiTest AT portable adhesion tester respectively. The wear test was performed by using pin-on-disk equipment and field emission scanning electron microscope (FESEM) used to determine the extent of surface damage. From the results obtained, mechanical properties such as hardness and adhesion strength of titanium (Ti) coating decreased with the increased of HA contents. Meanwhile, the coefficient of friction of Ti-10% HA coating shows the highest value compare to others as three-body abrasion had occurred during the test.

Magneto-optical and catalytic properties of Fe{sub 3}O{sub 4}@HA@Ag magnetic nanocomposite

Energy Technology Data Exchange (ETDEWEB)

Amir, Md., E-mail: mdamir01031001@gmail.com [Department of Chemistry, Fatih University, 34500 B.Çekmece-İstanbul, Turkey (Turkey); Bio & Nanotechnology Engineering, Faculty of Engineering, Istanbul University, 34452 Beyazıt/Fatih-İstanbul (Turkey); Güner, S. [Department of Physics, Fatih University, 34500 B.Çekmece-İstanbul, Turkey (Turkey); Yıldız, A. [Department of Textile Engineering, Namık Kemal University, 59860 Çorlu-Tekirdağ, Turkey (Turkey); Baykal, A. [Department of Chemistry, Fatih University, 34500 B.Çekmece-İstanbul, Turkey (Turkey)

2017-01-01

Fe{sub 3}O{sub 4}@HA@Ag magnetic nanocomposites (MNCs) were successfully synthesized by the simple reflux method for the removal of azo dyes from the industrial aqueous media. Fe{sub 3}O{sub 4}@HA@AgMNCs exhibited high catalytic activity to reduce MB within 20 min from the waste water. The obtained materials were characterized by the means of different techniques. Powder X-ray diffraction (XRD) analysis confirmed the single-phase of Fe{sub 3}O{sub 4} spinel structure. SEM and TEM analysis indicated that Fe{sub 3}O{sub 4}@HA@AgMNCs were nanoparticles like structure with small agglomeration. TG result showed that the products contained 9% of HA. The characteristic peaks of HA at 1601 cm{sup −1} and 1703 cm{sup −1} was observed by the means of FT-IR spectra of Fe{sub 3}O{sub 4}@HA@AgMNCs. The hysteresis (σ–H) curves revealed Fe{sub 3}O{sub 4}@HA@Ag MNCs exhibit a typical superparamagnetic characteristic with a saturation magnetization of 59.11 emu/g and measured magnetic moment is 2.45 µ{sub B}. The average magnetic particle dimension (D{sub mag}) is 13.25 nm. In accordance, the average crystallite and particle dimensions were obtained as 11.50 nm and 13.10 nm from XRD and TEM measurements, respectively. Magnetocrystalline anisotropy was offered as uniaxial and calculated effective anisotropy constant (K{sub eff}) is 2.96×10{sup 5} Erg/g. The blocking temperature was estimated as 522 K. The size-dependent saturation magnetization suggests the existence of a magnetically dead layer as 0.793 nm for Fe{sub 3}O{sub 4}@HA@Ag MNCs. The UV–vis diffuse reflectance spectroscopy (DRS) and Kubelka–Munk theory were applied to determine the optical properties of powder samples. The direct optical energy band gap (E{sub g}) values were estimated from Tauc plots between 1.62 eV and 2.12 eV. - Graphical abstract: An illustration for the fabrication of Fe{sub 3}O{sub 4}@HA@Ag MNCs. - Highlights: • The reduction of azo dye such Methylene Blue was instantly completed with

Antibiotic-containing hyaluronic acid gel as an antibacterial carrier: Usefulness of sponge and film-formed HA gel in deep infection.

Science.gov (United States)

Matsuno, Hiroaki; Yudoh, Kazuo; Hashimoto, Masamichi; Himeda, Yasukazu; Miyoshi, Teruzo; Yoshida, Kaoru; Kano, Syogo

2006-03-01

We have developed a novel bioabsorbable antibacterial carrier using hyaluronic acid (HA) gel for prevention and treatment of orthopedic infections. In this study, we investigated the in vivo antibacterial effects of two forms of this new material, an HA gel sponge and an HA gel film. A titanium cylinder was inserted into the intramedullary cavity of each rabbit femur, along with an HA gel sponge or HA gel film containing antibiotics. The HA gel sponge contained gentamycin, vancomycin, tobramycin, or minomycin. The HA gel film contained gentamycin or vancomycin. After 0, 7, and 14 days, the rabbit bone marrow was collected, and the antibacterial activity of the HA gel was determined by agar diffusion test. As a control, we used Septocoll, a commercially available antibacterial carrier. Both the HA gel sponge and HA gel film exhibited antibacterial activity. The present results indicate that HA gel containing antibiotics is a clinically useful bioabsorbable antibacterial carrier. Copyright 2006 Orthopaedic Research Society.

Bone Tissue Response to Nano-HA-ZrO2 Compound Bioceramics%纳米增韧HA-ZrO2生物陶瓷与骨缺损断面的结合性能观察

Institute of Scientific and Technical Information of China (English)

孟志兵; 唐月军; 郭军; 吕春堂; 周中华

2013-01-01

目的:观察纳米增韧HA-ZrO2生物陶瓷植入体与骨断面的结合情况.方法:12只健康成年新西兰大白兔,双侧下颌骨制备洞穿性骨缺损,一侧植入纳米增韧HA-ZrO2生物陶瓷,对侧植入HA(羟基磷灰石)材料,4、8、12周时取材,进行X线摄片观察、材料顶出实验、硬组织切片,比较观察2组骨与材料界面结合情况.结果:X线片和硬组织切片观察结果显示4、8周时HA-ZrO2、HA和骨断面结合不良,12周时发现两种材料均和骨断面结合良好,少部分区域有纤维组织包绕.4、8、12周纳米增韧HA-ZrO2组抗剪切强度分别为(2.36±1.04)、(10.65±1.48)和(30.95±3.67)MPa,不同时间段的组内差异有统计学意义;4、8、12周HA组抗剪切强度分别为(2.27±1.12)、(12.33±1.57)和(31.05±3.44)MPa,不同时间段组内比较差异有统计学意义(P＜0.01);但同时间段的结果组间比较,差异无统计学意义(P＞0.05).结论:初步认为纳米增韧HA-ZrO2生物陶瓷和骨断面,可以取得与HA类似的较好结合,但是如何在尽量短的时间内使其与骨断面完全结合,还需要进一步的研究.%Objective:The purpose of the present study was to observe the combination of the interface between nanoHA-ZrO2 compound bioceramics and bone tissue.Methods:12 healthy adult New Zealand rabbits were used in the study.Nano-HA-ZrO2 compound bioceramics were placed in one side of the mandible.On the other side,HA were placed as control.Animals were sacrificed at 4、8、12 weeks after operation,histological sections and the interface shear strength were evaluated.X-ray examination were also performed to observe the interface between the bone,nano-HA-ZrO2,and HA bioceramics materials.Results:Hard tissue section and X-rays examination showed a good interface osseointegration appeared at 12 weeks in nano-HA-ZrO2 ceramic group,as well as in HA group.Fibrous tissue manifested in some areas of the interface.Incomplete osseointegration were shown in the

Improving natural resource management in Viet Nam's Hong Ha ...

International Development Research Centre (IDRC) Digital Library (Canada)

2011-07-15

Jul 15, 2011 ... Improving natural resource management in Viet Nam's Hong Ha commune ... In this work, the research team helped local farmers improve their land ... with a home garden economy, limit forest destruction, diversify crop production, ... low female participation rates in decision making; limited education; rapid ...

Jom ha-Šo'a...počátky jednoho svátku

Directory of Open Access Journals (Sweden)

Zbyněk Tarant

2011-12-01

Full Text Available If one looks at the calendar, one will find there 'International Holocaust Rememberance Day' on 27th of January. This day was set by the UN General Assembly in 2005 and it refers to the anniversary of Auschwitz liberation. However, if one looks at the Israeli calendar, or any of the Jewish Luakh haShannah's, one will find there another day of mourning that is actually far older. The Yom HaShoah was set according to the Hebrew calendar and refers not to the liberation of Auschwitz, but to the Warsaw ghetto uprising. Today, this day of mourning is an interesting relict of the pioneer, upbuilding era that preferred the stories of heroes to those of victims. This article attempts to map the very beginning of the Yom HaShoah commemoration, by using Hebrew sources that were never published before in the Czech language. The story of Yom HaShoah in the context of the young state of Israel in the 1950s is an interesting case-study on the issue of how the contemporary political and public discourse affects the collective memory of  future generations.

DEMENCIA FRONTOTEMPORAL, CÓMO HA RESURGIDO SU DIAGNÓSTICO

Directory of Open Access Journals (Sweden)

Patricia Lillo Z, MD, PhD

2016-05-01

Full Text Available Desde la descripción inicial efectuada por Pick hace más de un siglo atrás, el interés por las demencias frontotemporales ha tenido un gran crecimiento. Gracias a los avances de las neuroimágenes y el desarrollo de biomarcadores, el progreso en este campo ha generado nuevos conocimientos en torno a la categorización clínica, correlatos neuronales de las funciones cerebrales superiores y mecanismos de neurodegeneración. Paradójicamente, estos avances han traído consigo cierta controversia y desafíos, los cuales han motivado la creación de nuevos criterios diagnósticos. Esta breve revisión muestra la evolución histórica de estas condiciones y describe los criterios diagnósticos actuales para la variante conductual y las afasias progresivas primarias, que son las dos formas clínicas de las demencias frontotemporales.

Functional study of the novel multidrug resistance gene HA117 and its comparison to multidrug resistance gene 1

Directory of Open Access Journals (Sweden)

Chen Tingfu

2010-07-01

Full Text Available Abstract Background The novel gene HA117 is a multidrug resistance (MDR gene expressed by all-trans retinoic acid-resistant HL-60 cells. In the present study, we compared the multidrug resistance of the HA117 with that of the classical multidrug resistance gene 1 (MDR1 in breast cancer cell line 4T1. Methods Transduction of the breast cancer cell line 4T1 with adenoviral vectors encoding the HA117 gene and the green fluorescence protein gene (GFP (Ad-GFP-HA117, the MDR1 and GFP (Ad-GFP-MDR1 or GFP (Ad-GFP was respectively carried out. The transduction efficiency and the multiplicity of infection (MOI were detected by fluorescence microscope and flow cytometry. The transcription of HA117 gene and MDR1 gene were detected by reverse transcription polymerase chain reaction (RT-PCR. Western blotting analysis was used to detect the expression of P-glycoprotein (P-gp but the expression of HA117 could not be analyzed as it is a novel gene and its antibody has not yet been synthesized. The drug-excretion activity of HA117 and MDR1 were determined by daunorubicin (DNR efflux assay. The drug sensitivities of 4T1/HA117 and 4T1/MDR1 to chemotherapeutic agents were detected by Methyl-Thiazolyl-Tetrazolium (MTT assay. Results The transducted efficiency of Ad-GFP-HA117 and Ad-GFP-MDR1 were 75%-80% when MOI was equal to 50. The transduction of Ad-GFP-HA117 and Ad-GFP-MDR1 could increase the expression of HA117 and MDR1. The drug resistance index to Adriamycin (ADM, vincristine (VCR, paclitaxel (Taxol and bleomycin (BLM increased to19.8050, 9.0663, 9.7245, 3.5650 respectively for 4T1/HA117 and 24.2236, 11.0480, 11.3741, 0.9630 respectively for 4T1/MDR1 as compared to the control cells. There were no significant differences in drug sensitivity between 4T1/HA117 and 4T1/MDR1 for the P-gp substrates (ADM, VCR and Taxol (P Conclusions These results confirm that HA117 is a strong MDR gene in both HL-60 and 4T1 cells. Furthermore, our results indicate that the MDR

Sugar-binding sites of the HA1 subcomponent of Clostridium botulinum type C progenitor toxin.

Science.gov (United States)

Nakamura, Toshio; Tonozuka, Takashi; Ide, Azusa; Yuzawa, Takayuki; Oguma, Keiji; Nishikawa, Atsushi

2008-02-22

Clostridium botulinum type C 16S progenitor toxin contains a hemagglutinin (HA) subcomponent, designated HA1, which appears to play an important role in the effective internalization of the toxin in gastrointestinal epithelial cells and in creating a broad specificity for the oligosaccharide structure that corresponds to various targets. In this study, using the recombinant protein fused to glutathione S-transferase, we investigated the binding specificity of the HA1 subcomponent to sugars and estimated the binding sites of HA1 based on X-ray crystallography and soaking experiments using various sugars. N-Acetylneuraminic acid, N-acetylgalactosamine, and galactose effectively inhibited the binding that occurs between glutathione S-transferase-HA1 and mucins, whereas N-acetylglucosamine and glucose did not inhibit it. The crystal structures of HA1 complex with N-acetylneuraminic acid, N-acetylgalactosamine, and galactose were also determined. There are two sugar-binding sites, sites I and II. Site I corresponds to the electron densities noted for all sugars and is located at the C-terminal beta-trefoil domain, while site II corresponds to the electron densities noted only for galactose. An aromatic amino acid residue, Trp176, at site I has a stacking interaction with the hexose ring of the sugars. On the other hand, there is no aromatic residue at site II; thus, the interaction with galactose seems to be poor. The double mutant W176A at site I and D271F at site II has no avidity for N-acetylneuraminic acid but has avidity for galactose. In this report, the binding specificity of botulinum C16S toxin HA1 to various sugars is demonstrated based on its structural features.

Serum expression of HA and LN in lewis rat models of autoimmune myocarditis

International Nuclear Information System (INIS)

Han Li'na; Li Tieling; Zhang Yajing; Yang Tingshu; Ding Yu; Guo Shuli; Zhao Xiaoning

2011-01-01

Objective: To study the clinical significance of dynamic changes of serum expressions of hyaluronic acid (HA) and laminin (LN) in Lewis rat models of experimental autoimmune myocarditis (EAM). Methods: Fifty Lewis rat models of experimental autoimmune myocarditis (EAM) were established with injection of recombinant cardiac C protein with complete freund adjuvant into two foot-pads plus intraperitoneal injection of pertussis toxin. At 1w, 2w, 4w, 6w and 8w, 10 models were sacrificed each time;cardiac tissues were examined with HE stain for myocardial inflammatory score and examined with picrosirius red stain for myocardial fibrosis score, also, serum HA and LN expressions were determined with RIA. These examinations were performed in 10 undisturbed animals as controls. Results: The myocardial inflammatory scores of the models at 1w were about the same as those in the controls, but the scores rapidly increased from 2w on to 4w then fell gradually. The myocardial fibrosis scores of the models at 1wk were also not much different from those in controls. The fibrosis scores increased rapidly at 4w and maintained at high level up to 8w. The changes of serum expressions of HA and LN roughly paralleled those of myocardial fibrosis scores i. e. rapidly increased at 4w up to 8w. Conclusion: Serum expressions of HA and LN could faithfully reflect the degree of myocardial fibrosis in rat models of EAM. HA and LN were useful markers of myocardial fibrois and were of prognostic importance. (authors)

3D-printed scaffolds based on PLA/HA nanocomposites for trabecular bone reconstruction

Science.gov (United States)

Niaza, K. V.; Senatov, F. S.; Kaloshkin, S. D.; Maksimkin, A. V.; Chukov, D. I.

2016-08-01

In the present work porous PLA scaffolds filled with micro- and nano- HA were studied. Both composites with micro- and nano-HA were obtained by extrusion in the same conditions. Scaffolds were obtained by 3D-printing by fused filament fabrication method. Structure of porous scaffolds was pre-modeled by computer software. Compression and three - point flexural tests were used to study mechanical properties of the scaffolds.

Role of induced glutathione-S-transferase from Helicoverpa armigera (Lepidoptera: Noctuidae) HaGST-8 in detoxification of pesticides.

Science.gov (United States)

Labade, Chaitali P; Jadhav, Abhilash R; Ahire, Mehul; Zinjarde, Smita S; Tamhane, Vaijayanti A

2018-01-01

The present study deals with glutathione-S-transferase (GST) based detoxification of pesticides in Helicoverpa armigera and its potential application in eliminating pesticides from the environment. Dietary exposure of a pesticide mixture (organophosphates - chlorpyrifos and dichlorvos, pyrethroid - cypermethrin; 2-15ppm each) to H. armigera larvae resulted in a dose dependant up-regulation of GST activity and gene expression. A variant GST from H. armigera (HaGST-8) was isolated from larvae fed with 10ppm pesticide mixture and it was recombinantly expressed in yeast (Pichia pastoris HaGST-8). HaGST-8 had a molecular mass of 29kDa and was most active at pH 9 at 30°C. GC-MS and LC-HRMS analysis validated that HaGST-8 was effective in eliminating organophosphate type of pesticides and partially reduced the cypermethrin content (53%) from aqueous solutions. Unlike the untransformed yeast, P. pastoris HaGST-8 grew efficiently in media supplemented with pesticide mixtures (200 and 400ppm each pesticide) signifying the detoxification ability of HaGST-8. The amino acid sequence of HaGST-8 and the already reported sequence of HaGST-7 had just 2 mismatches. The studies on molecular interaction strengths revealed that HaGST-8 had stronger binding affinities with organophosphate, pyrethroid, organochloride, carbamate and neonicotinoid type of pesticides. The abilities of recombinant HaGST-8 to eliminate pesticides and P. pastoris HaGST-8 to grow profusely in the presence of high level of pesticide content can be applied for removal of such residues from food, water resources and bioremediation. Copyright © 2017 Elsevier Inc. All rights reserved.

REGGIO EMILIA, LUGAR DONDE LA INFANCIA VIVE LA CIENCIA

Directory of Open Access Journals (Sweden)

Lilian Narváez Prosser

2009-01-01

Full Text Available La ciudad italiana de Reggio Emilia, situada a 60 kilómetros de Bolonia que alberga a poco más de 140.000 personas, fue punto de encuentro con especialistas en Educación de la primera infancia, quienes compartieron el conocimiento, in situ, de la metodología de trabajo que Loris Malaguzzy creó e implementó en dicha ciudad y que ha sido reconocida a nivel mundial como la mejor calidad de trabajo en la primera infancia en todo el mundo. Dicha metodología está basada en su ley fundamental de la realidad, la que su creador clarifi ca al escribir "Si se hacen cosas reales, también son reales sus consecuencias" en el respeto, en donde la construcción del pensamiento y la ciencia como proyectos de trabajo son elementos permanentes en una pedagogía relacional con agentes educativos activos y comprometidos que incluye a la comunidad en pleno.

Bør leger ha reservasjonsrett ved assistert befruktning?

Directory of Open Access Journals (Sweden)

Morten Magelssen

2011-10-01

Full Text Available Omfanget av helsepersonells reservasjonsrett har nylig vært gjenstand for debatt i Norge. Vi spør om leger bør ha reservasjonsrett ved utførelse og henvisning til assistert befruktning, og drøfter argumenter for og imot ved hjelp av et rammeverk med sju kriterier for vurdering av reservasjon. Reservasjonsrettens grunnleggende dilemma er hvordan to viktige hensyn, henholdsvis pasientens rett til behandling og hensynet til helsepersonellets moralske integritet, best kan ivaretas. Det argumenteres for at leger bør ha rett til å reservere seg mot å utføre, assistere ved og henvise til assistert befruktning generelt hvis begrunnelsen er hensynet til befruktede eggs moralske verdi. Videre finner vi at leger også kan ha en moralsk rett til reservasjon mot å utføre, assistere ved og henvise til assistert befruktning for likekjønnede, men da på nærmere spesifiserte vilkår.Nøkkelord: reservasjonsrett, assistert befruktning, samvittighet, moralsk integritetEnglish summary: Should physicians have the right to conscientiously object to assisted reproduction?The extent of the healthcare worker's right to conscientious objection has recently been debated in Norway. This article asks whether physicians should have a right to conscientious objection to the performance of, and referral for, assisted reproduction, and discusses arguments for and against the same, utilizing a framework of seven criteria for the evaluation of conscientious objection. The fundamental dilemma of conscientious objection is how two important considerations can be reconciled: the patient's right to treatment, and the protection of the healthcare worker's moral integrity. It is argued that physicians should have the right to object to performing, assisting with, and referring for assisted reproduction generally when the objection is grounded in the moral value of the embryo. Furthermore, physicians may also have a moral right to object to performing, assisting with, and

[Study on biocompatibility of hydroxyapatite/high density polyethylene (HA/HDPE) nano-composites artificial ossicle].

Science.gov (United States)

Wang, Guohui; Zhu, Shaihong; Tan, Guolin; Zhou, Kechao; Huang, Suping; Zhao, Yanzhong; Li, Zhiyou; Huang, Boyun

2008-06-01

This study was aimed to evaluate the biocompatibility of Hydroxyapatite/High density polyethylene (HA/ HDPE) nano-composites artificial ossicle. The percentage of S-period cells were detected by flow cytometry after L929 cells being incubated with extraction of the HA/HDPE nano-composites; the titanium materials for clinical application served as the contrast. In addition, both materials were implanted in animals and the histopathological evaluations were conducted. There were no statistically significant differences between the two groups (P >0.05). The results demonstrated that the HA/HDPE nano-composite artificial ossicle made by our laboratory is of a good biocompatibility and clinical application outlook.

HaNDL Syndrome Presenting During Pregnancy: A Case Report and Review of the Literature

Directory of Open Access Journals (Sweden)

Yüksel Kaplan

2014-09-01

Full Text Available Headache associated with neurological deficits and cerebrospinal fluid lymphocytosis (HaNDL is a self-limited syndrome characterized by sudden-onset headache with a temporary neurological deficit and cerebrospinal fluid (CSF lymphocytosis. We aimed to disscus a case of HaNDL syndrome presenting during pregnancy with relevant literature. A 20-year-old female presented with a 5-day history of severe, bilateral throbbing headache accompanied by nausea, vomiting, and phonophobia. Approximately 2 days after the pain developed, she became acutely confused for less than 90 minutes. 2 days after this episode, she experienced again confusional state and left hemiparesis. There were no symptoms consistent with meningoencephalitis. She was pregnant and at 11 weeks gestation. A neurologic examination showed confusional state, bilateral papilledema, and mild left hemiparesis. The neuroradiological examination was normal. The cerebrospinal fluid revealed lymphocytic pleocytosis, mildly elevated protein, and increased opening pressure. She recovered completely after 8 days. The precise etiology of HaNDL is unknown, although an inflammatory or infectious origin and autoimmune factors have been proposed. Moreover, the risk factors and medical conditions associated with HaNDL are unknown. It is obviously difficult to determine whether the pregnancy was coincidental or associated in this case. We believe that comprehensive studies are needed to clarify the risk factors and medical conditions associated with HaNDL

Neural stem cell proliferation and differentiation in the conductive PEDOT-HA/Cs/Gel scaffold for neural tissue engineering.

Science.gov (United States)

Wang, Shuping; Guan, Shui; Xu, Jianqiang; Li, Wenfang; Ge, Dan; Sun, Changkai; Liu, Tianqing; Ma, Xuehu

2017-09-26

Engineering scaffolds with excellent electro-activity is increasingly important in tissue engineering and regenerative medicine. Herein, conductive poly(3,4-ethylenedioxythiophene) doped with hyaluronic acid (PEDOT-HA) nanoparticles were firstly synthesized via chemical oxidant polymerization. A three-dimensional (3D) PEDOT-HA/Cs/Gel scaffold was then developed by introducing PEDOT-HA nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. HA, as a bridge, not only was used as a dopant, but also combined PEDOT into the Cs/Gel via chemical crosslinking. The PEDOT-HA/Cs/Gel scaffold was used as a conductive substrate for neural stem cell (NSC) culture in vitro. The results demonstrated that the PEDOT-HA/Cs/Gel scaffold had excellent biocompatibility for NSC proliferation and differentiation. 3D confocal fluorescence images showed cells attached on the channel surface of Cs/Gel and PEDOT-HA/Cs/Gel scaffolds with a normal neuronal morphology. Compared to the Cs/Gel scaffold, the PEDOT-HA/Cs/Gel scaffold not only promoted NSC proliferation with up-regulated expression of Ki67, but also enhanced NSC differentiation into neurons and astrocytes with up-regulated expression of β tubulin-III and GFAP, respectively. It is expected that this electro-active and bio-active PEDOT-HA/Cs/Gel scaffold will be used as a conductive platform to regulate NSC behavior for neural tissue engineering.

Belgia rahvuspüha puhul kuuleb Eestis kammermuusikat / Maarja Pehk

Index Scriptorium Estoniae

Pehk, Maarja

2007-01-01

21. juulil on Belgia Kuningriigil rahvuspüha. Suursaadik Pierre Clement Dubuisson tutvustab sel puhul belgia kultuuri kolme kontserdi kaudu. Sel aastal tutvustab saatkond Belgia kultuuri ka koostöös Pimedate Ööde filmifestivaliga

3D-printed scaffolds based on PLA/HA nanocomposites for trabecular bone reconstruction

International Nuclear Information System (INIS)

Niaza, K V; Senatov, F S; Kaloshkin, S D; Maksimkin, A V; Chukov, D I

2016-01-01

In the present work porous PLA scaffolds filled with micro- and nano- HA were studied. Both composites with micro- and nano-HA were obtained by extrusion in the same conditions. Scaffolds were obtained by 3D-printing by fused filament fabrication method. Structure of porous scaffolds was pre-modeled by computer software. Compression and three - point flexural tests were used to study mechanical properties of the scaffolds. (paper)

Ganándole la batalla al mal humor. Una propuesta didáctica

Directory of Open Access Journals (Sweden)

Sarah Díaz García

2012-01-01

Full Text Available En los últimos años, el aprendizaje de lenguas extranjeras ha llegado a ser una necesidad real tanto profesional como personal para todos nosotros como ciudadanos y como trabajadores. La enseñanza del español como lengua extranjera, que es el área de estudio en el que nos centraremos en el resto del presente artículo, ha evolucionado a medida que nuestra sociedad ha ido cambiando y adaptándose a los nuevos tiempos. Este cambio surgió con el Marco Común Europeo de Referencia para las Lenguas (MCER; pasamos de darle una máxima importancia a los aspectos puramente lingüísticos a abrirnos al hecho de que aprender un segundo idioma no es solo centrarnos en la gramática. La competencia comunicativa, por ejemplo, ha ido cobrando protagonismo en los últimos años; la idea de que un estudiante solo es competente en el uso de una lengua cuando utiliza la lingüística (pensemos en la gramática de forma correcta y siguiendo la norma ha caído en desaprobación para muchos. La realidad es que para que un alumno se considere competente en el uso de otra lengua que no sea la suya debe saber utilizar las técnicas y recursos pertinentes para poder comunicarse efectivamente con su interlocutor en un contexto real de habla.

Study on the correlationship between serum IGF-I and HA, PIIIP levels in patients with chronic hepatitis C

International Nuclear Information System (INIS)

Li Yong; Sun Yefu; Xia Aiping

2008-01-01

Objective: To study the inter-relationship between serum IGF-I and HA, PIIIP levels in patients with chronic hepatitis C. Methods: Serum IGF-I, HA and PIIIP levels were determined with RIA in 39 patients with chronic hepatitis C and 35 controls. Results: Serum IGF-I, HA and PIIIP levels in the patients were significantly higher than those in controls (P<0.01). The serum IGF-I levels and HA, PIIIP levels were mutually positively correlated (r=0.8018, 0.7126, P<0.01). Conclusion: Serum IGF-I, HA and PIIIP were useful diagnostic markers in patients with chronic hepatitis C. (authors)

Tallinna Püha hõimkonna altariretaabel / Merike Koppel ; tõlk. Tiina Randviir, toim. Richard Adang

Index Scriptorium Estoniae

Koppel, Merike

2006-01-01

Tallinna Niguliste kirikus asuva 17. sajandi keskpaigas ümber tehtud hiliskeskaegse Püha hõimkonna altariretaabli algne pildiprogramm. Altariretaabli korpuse rekonstruktsioon. Kappaltari algse asukoha ja võimalike tellijate väljaselgitamisest. Püha hõimkond Flandria hiliskeskaegsetel nikerdaltaritel

HDPE/HA composites obtained in solution: Effect of the gamma radiation

International Nuclear Information System (INIS)

Carmen, Albano; Arquimedes, Karam; Rosestela, Perera; Gema, Gonzalez; Nohemy, Dominguez; Jeanette, Gonzalez; Yanixia, Sanchez

2006-01-01

Radiation is employed to sterilize composite materials used in the biomedical field. Due to the changes induced by radiation onto polymeric materials, it is important to study variations in their melt flow index (MFI), as well as in their mechanical and thermal properties. In this work, those previous parameters were determined in composites obtained via solution of a high-density polyethylene (HDPE) in decalin, with different amounts of hydroxyapatite (HA), varying from 10 to 30 parts per hundred, after being exposed to gamma radiation at absorbed doses between 25 and 100 kGy. After the irradiation, the MFI of HDPE dissolved in decalin and precipitated afterwards and without filler increased from 6 to 24 g/10 min at the highest absorbed doses. This behavior was also observed in composites with 10 pph of HA, being the increase less pronounced, specifically in the range between 50 and 100 kGy. Composites with 20 and 30 pph of HA showed a maximum MFI value at 50 kGy, which decreased at higher doses. This implies that the filler begin to exert an influence because it does not melt at the test temperature and consequently, it does not flow. It was observed that Young's modulus increased with HA addition due to rigidity of the ceramic filler. Radiation did not significantly affect this tensile property. On the other hand, the tensile strength did not show significant variations at the different doses but the filler content did affect this property improving it. Finally, elongation at break showed a drastic decrease with filler addition. When the thermal behavior was studied it was noticed that crystallization and melting temperatures remained unchanged. Instead, crystallinity degree slightly increased in the composites, and a little decrease was obtained when they were irradiated

Telemedicine perspectives in medical emergencies Perspectivas de la telemedicina en las emergencias y urgencias médicas

Directory of Open Access Journals (Sweden)

Antonio Cuadrado Ruiz

2001-03-01

Full Text Available Telemedicine already exists in emergency departments. The development of HECTOR and WETS projects in Andalusia (Spain is a proof of that. This review article shows how new technologies support changes in the traditional form of medical practice and health care services. Our minds must change as times change. La telemedicina ya ha llegado a los departamentos de urgencias y emergencias. El desarrollo de proyectos como el HECTOR y el WETS, actualmente en ejecución en Andalucía (España son buena prueba de ello. Este artículo de revisión muestra como las nuevas tecnologías van apoyando cambios en la práctica tradicional de los servicios de salud. Nuestros pensamientos deben cambiar cuando los tiempos cambian.

Study on the changes of serum HA and CG levels in several diseases besides liver disorders

Energy Technology Data Exchange (ETDEWEB)

Zhiping, Lu; Yunbao, Ma; Xiaoyi, Zhang; Weihua, Lv [Changshu Hospital Attached to Medical College of Yangzhou Univ., Changshu No.2 People' s Hospital, Changshu (China)

2005-12-15

Objective: To study the changes of serum hyaluronic acid (HA) and cholyglycine (CG) in several diseases besides liver disorders. Methods: Serum HA and CG levels were measured with RIA in 78 patients with chronic liver diseases (with 70 controls), (84 patients with primary hepatic carcinoma ), 70 pediatric patients with various infections diseases (with 40 controls), 50 pediatric patients with recurrent respiratory infection (RRI, with 30 controls) and 428 pregnant women ( with 60 controls). In addition, RBC C{sub 3}b receptor ratio (RBCC{sub 3} bRR) and RBC immune-complex ratio (RBCICR) (both with yeast rosette method) as well as serum IgG,IgM,IgA,C{sub 3} levels (with immunoturbidity test) were examined in the 50 RRI pediatric patients, ALT levels were examined in the 70 pediatric patients with infectious diseases and SF examined in the 78 patients with chronic liver diseases. Results: The serum CG, HA and SF levels in the three groups of patients with chronic liver diseases ( CPH, CAH, Cirrhosis) were significantly higher than those in the controls (all P<0.01 ) with the exception of HA in CPH patients (P > 0.05). In patients with hepatic carcinoma, the CG and HA levels were positively correlated with the mortality at 4 months (P < 0.05). Among pregnant women, serum levels of HA and CG were significantly higher during the second and third trimesters (P<0.05-0.01) than those in the controls but not so during the first trimester. In the pediatric patients with infectious diseases, CG and HA levels were significantly higher in all these patients (P<0.01) with the only exception of HA in patients with mumps (P>0.05). For ALT, levels only increased in patients with hepatitis and typhoid fever with none in bacillary dysentery and mumps. In pediatric patients with RRI, RBCC{sub 3}bRR (%), CG, C{sub 3}, IgG levels were significantly higher than those in controls (P<0.05 ) with no significant differences in the case of RBCICR (%), HA, IgM and IgA levels (P>0

HA Cells monitoring at the Underground Research Laboratory (URL) in the CMHM (Andra)

International Nuclear Information System (INIS)

Gay, Olivier; Allagnat, Dominique; Morel, Jacques; Armand, Gilles

2010-01-01

The experimental monitoring program of the HA (High Activity) cells was carried out at the Underground Research Laboratory (URL) in the Meuse Haute Marne department in France (CMHM Andra). Inspections made by video and photographs, section measurements and geo-referenced trajectories, in addition to measurements of convergence, temperature and hygrometry over time, allowed a better analysis of the behaviour of the HA cells after excavation, and subsequently over the long term. (authors)

Propuesta de un plan de desarrollo integral del guanábano (Annona muricata L. en el estado de Veracruz México

Directory of Open Access Journals (Sweden)

Librado Vidal Hernández

2014-01-01

Full Text Available El estado de Veracruz tiene una superficie de 71' 227 km². Cuenta con una zona potencialmente apta para el cultivo del guanábano de 18' 440 ha, (0.21%, una zona medianamente propicia de 3' 645 324 ha (51.30% y una zona no apropiada de 3' 458 862 ha, (48.44%. Existen 20 municipios productores de guanábano en el estado de Veracruz. Actualmente la demanda por este producto ha permitido su incremento en superficie estimándose en 800 ha, en estos últimos años. Con un rendimiento aproximado de 5.0 ton/ha, por debajo de la media nacional que es de 6.5 ton/ha, esto refleja la poco tecnología empleada en el manejo del cultivo. Lamentablemente el desarrollo de este frutal en Veracruz se ha realizado de una manera desordenada. Todo ello, sin ninguna planeación y sin un estudio sobre un ordenamiento agroecológico a fin de detectar áreas potencialmente aptas para este cultivo. A pesar de toda esta complejidad se ha llegado a considerar como un frutal digno de atención por las posibilidades agroindustriales que representa. En general son tres los principales puntos prioritarios a tomar en cuenta para esta estrategia de desarrollo: Primero las características genéticas del material de propagación. Segundo las condiciones de sanidad de las plantas, principal factor que podría ser limitativo para el desarrollo del guanábano. Tercero la tecnología de producción. Existe desconocimiento en la lámina e intervalo riego, época; dosis y fuente de fertilización; época y tipo de poda. Existe una gran fortaleza en su aprovechamiento integral de este frutal: comercial, industrial, medicinal, farmacéutico, fitotóxico, alimenticio, entre otras propiedades.

Augmenter le taux de vaccination et réduire la mortalité infantile à Haïti

International Development Research Centre (IDRC) Digital Library (Canada)

2 mai 2016 ... Résultats de l'Initiative canadienne d'immunisation internationale pour Haïti À Haïti, un enfant sur quatorze ne survit pas assez longtemps pour fêter son premier anniversaire. Parmi les facteurs contributifs, citons les taux extrêmement faibles de vaccination à Haïti, qui sont parmi les plus bas au monde.

Preparation and properties of polyvinyl alcohol (PVA) and hydroxylapatite (HA) hydrogels for cartilage tissue engineering.

Science.gov (United States)

Yuan, F; Ma, M; Lu, L; Pan, Z; Zhou, W; Cai, J; Luo, S; Zeng, W; Yin, F

2017-05-20

A novel bioactive hydrogel for cartilage tissue based on polyvinyl alcohol (PVA) and hydroxylapatite (HA) were prepared, the effects of its component contents on the mechanical properties and microstructure of the hydrogel were investigated. The important properties of the scaffold composites, such as density, porosity, compressive modulus and microstructure were studied and analyzed through various measurements and methods. The biodegradability of hydrogel was evaluated by soaking the samples into artificial degradation solution at body temperature (36 - 37 oC) in vitro. Experimental results showed that the PVA/HA hydrogels had a density of 0.572 - 0.683 g/cm3, a porosity of 63.25 - 96.14% and a compressive modulus of 5.62 - 8.24 MP. The HA compound in the hydrogels enhanced the biodegradation significantly and linearly increased the rate of biodegradation by 2.3 - 8.5 %. The compressive modulus of PVA/HA exhibited a linear reduce to 0.86 - 1.53 MP with the time of degradation. The scaffold composites PVA/HA possess a high porosity, decent compressive modulus and good biodegradability. After further optimizing the structure and properties, this composite might be considered as novel hydrogel biomaterials to be applied in the field of cartilage tissue engineering.

Characterization and corrosion property of nano-rod-like HA on fluoride coating supported on Mg-Zn-Ca alloy.

Science.gov (United States)

Feng, Yashan; Zhu, Shijie; Wang, Liguo; Chang, Lei; Yan, Bingbing; Song, Xiaozhe; Guan, Shaokang

2017-06-01

The poor corrosion resistance of biodegradable magnesium alloys is the dominant factor that limits their clinical application. In this study, to deal with this challenge, fluoride coating was prepared on Mg-Zn-Ca alloy as the inner coating and then hydroxyapatite (HA) coating as the outer coating was deposited on fluoride coating by pulse reverse current electrodeposition (PRC-HA/MgF 2 ). As a comparative study, the microstructure and corrosion properties of the composite coating with the outer coating fabricated by traditional constant current electrodeposition (TED-HA/MgF 2 ) were also investigated. Scanning electron microscopy (SEM) images of the coatings show that the morphology of PRC-HA/MgF 2 coating is dense and uniform, and presents nano-rod-like structure. Compared with that of TED-HA/MgF 2 , the corrosion current density of Mg alloy coated with PRC-HA/MgF 2 coatings decreases from 5.72 × 10 -5 A/cm 2 to 4.32 × 10 -7 A/cm 2 , and the corrosion resistance increases by almost two orders of magnitude. In immersion tests, samples coated with PRC-HA/MgF 2 coating always show the lowest hydrogen evolution amount, and could induce deposition of the hexagonal structure-apatite on the surface rapidly. The results show that the corrosion resistance and the bioactivity of the coatings have been improved by adopting double-pulse current mode in the process of preparing HA on fluoride coating, and the PRC-HA/MgF 2 coating is worth of further investigation.

Screening of the target genes trans-activated by HLA-HA8 in hepatocytes

Directory of Open Access Journals (Sweden)

Qi WANG

2011-06-01

Full Text Available Objective To clone and identify the target genes trans-activated by human minor histocompatibility antigen HLA-HA8 in hepatocytes with suppression subtractive hybridization(SSH and bioinfomatics technique.Methods mRNA was isolated from HepG2 cells transfected by pcDNA3.1(--HLA-HA8 and pcDNA3.1(- empty vector,and then used to synthesize the double-stranded cDNA(marked as Tester and Driver,respectively by reverse transcription.After being digested with restriction enzyme Rsa I,the tester cDNA was divided into two parts and ligated to the specific adaptor 1 and adaptor 2,respectively,and then hybridized with driver cDNA twice and underwent PCR twice.The production was subcloned into pEGM-Teasy plasmid vectors to set up the subtractive library.The library was then amplified by transfection into E.coli strain DH5α.The cDNA was sequenced and analyzed in GenBank with Blast search after PCR amplification.Results The subtractive library of genes trans-activated by HLA-HA8 was constructed successfully.The amplified library contained 101 positive clones.Colony PCR showed that all these clones contained 200-1000bp inserts.Twenty eight clones were selected randomly to analyze the sequences.The result of homologous analysis showed that altogether 16 coding sequences were gotten,of which 4 sequences were with unknown function.Conclusions The obtained sequences trans-activated by HLA-HA8 may code different proteins and play important roles in cell growth and metabolism,energy synthesis and metabolism,material transport and signal transduction.This finding will bring some new clues for the studies not only on the biological functions of HLA-HA8,but also on the HBV infection mechanism.

¿Ha llegado la hora de la gestión de las listas de espera? Has the time arrived for the management of waiting lists?

Directory of Open Access Journals (Sweden)

E. Bernal

2002-10-01

Full Text Available Las personas que ocupan una lista de espera sufren a menudo un riesgo adicional derivado del tiempo que pasa hasta que obtienen tratamiento; sin embargo, en otras ocasiones, las personas en lista no tienen necesidad del tratamiento por el que esperan. Ambos argumentos, contrastables con evidencias empíricas, serían suficientes para afirmar que debe llegar la gestión a las listas de espera dejando a un lado políticas más o menos oportunistas. Por políticas oportunistas se entiende mantener la mala información sobre listas o su "maquillaje", utilizar programas de autoconcertación sin más horizonte que llegar a final de año sin lista de más de seis meses, etcétera. El panorama no es del todo oscuro. Algunas iniciativas de gestión (incluso de Política con mayúscula se van abriendo paso y pueden entrar en la agenda de los próximos años. Así, cabe destacar la aplicación de tiempos de atención garantizada o la priorización de las listas en función de criterios explícitos. En todo caso, conviene recordar que, con la excepción de las colas producidas en las salas de espera de los centros de salud y aquéllas que se producen en las puertas de urgencias, el resto de colas del sistema están mediadas por la decisión de un médico. Así que una estrategia ineludible para gestionar las listas de espera consiste en atenuar los problemas derivados de la incertidumbre (o ignorancia con respecto al diagnóstico o al pronóstico de los pacientes.Individuals on the waiting list frequently suffer an additional risk caused by the mean time until they receive treatment; however, other individuals do not need the treatment for which they are waiting. Both arguments, which can be contrasted with empirical evidence, would be sufficient to affirm that waiting list management should be implemented, leaving aside policies that are more of less opportunistic. Opportunistic policies are understood as those providing misinformation on waiting lists or their "manipulation", and using programs of auto-coordination with the sole aim of reaching the end of the year without a waiting list of not more than six months, etc. The panorama is not completely bleak. Some management initiatives (and even Politics with a capital P are opening the way forward and may enter the Agenda in the next few years. In this context, the application of guaranteed times of medical care or the prioritization of waiting lists according to explicit criteria should be highlighted. It is worth remembering that, except for the queues in the waiting rooms of health centers and emergency departments, waiting lists are mediated by the decision of the physician. Therefore, an essential strategy for managing waiting lists consists of attenuating the problems caused by uncertainty (or ignorance of the patient's diagnosis or prognosis.

La logística y sus componentes

Directory of Open Access Journals (Sweden)

Deyanira Elizabeth Argumedo Sánchez

2014-05-01

Full Text Available Hoy en día el tema de la logística es un tema de suma importancia para las empresas ya que estas se han dado a la tarea de crear áreas especificas para su análisis se ha ido desarrollando a través del tiempo y en la actualidad se ha convertido en un aspecto de suma importancia para lograr que la empresa se posicione como del primer mundo. Antes se entendía como logística el tener el producto en el lugar indicado en el tiempo justo al menor costo posible pero ahora esas actividades han llegado a ser mejoradas que no solo se trata de eso ahora son todo un proceso. La logística determina y coordina en forma óptima el producto correcto, el cliente correcto, el tiempo y lugar correctos por lo tanto la logística no es una actividad funcional si no un modelo que permitirá reducir la incertidumbre de un futuro desconocido.

Responsabilidad ambiental empresarial: el caso de "Hotelera Posadas"

Directory of Open Access Journals (Sweden)

Elva Esther Vargas Martínez

2012-01-01

Full Text Available Frente a los altos niveles de deterioro de la naturaleza, las organizaciones públicas y privadas han configurado una serie de acciones tendientes a la conservación del entorno, las cuales, en su conjunto, son denominadas gestión ambiental, cuyo propósito de implementación ha llegado a diferentes ámbitos incluyendo el turismo. El artículo expone a través de un estudio de caso cómo la Hotelera Posadas ha asumido su responsabilidad ambiental obedeciendo a dos intenciones: el compromiso empresarial que se tiene como la hotelera con más presencia en la República Mexicana y el hacer uso de la oportunidad invaluable para mejorar su posición competitiva ante la percepción pública, al reportar resultados ambientales; todo ello, en un marco de cooperación entre empresa y gobierno en donde la participación de la operadora hotelera en el Programa Nacional de Auditoría Ambiental se reconoce como una contribución al logro de la sustentabilidad.

Matrimonio poligámico, inmigración islámica y libertad de conciencia en España

Directory of Open Access Journals (Sweden)

Margarita Lema Tomé

2003-01-01

Full Text Available En este artículo se analiza el derecho a contraer nupcias en situaciones de inmigración, mostrando las diferencias ideológicas que pueden ocurrir en los Estados de origen y destino en la forma de concebir el matrimonio. El artículo se centra en la experiencia de los inmigrantes islámicos en España. Un tema especialmente difícil es el matrimonio poligámico, institución arraigada en los países islámicos que ha planteado varios problemas, especialmente desde la perspectiva jurídica. La solución a la que se ha llegado en España procede de la vía jurisprudencial y tiene como punto de referencia dos casos: el de Diop, un inmigrante senegalés que fallece dejando dos viudas, y el de un trabajador marroquí, que había estado casado con dos mujeres a la vez durante un periodo, quien muere tras haberse divorciado de una de ellas

Áreas metropolitanas:¿qué ha cambiado?: La experiencia de la Caracas Metropolitana

Directory of Open Access Journals (Sweden)

Sonia Barrios

2001-05-01

Full Text Available La nueva economía de la información y del conocimiento ha tenido tres decisivas implicaciones de orden territorial. Primero, ha acelerado el proceso de aglomeración urbana a escala planetaria. Segundo, ha provocado fuertes alteraciones en las redes urbanas de ámbito mundial, regional y nacional. Y tercero, ha impulsado la reestructuración interna de las áreas metropolitanas que se habían conformado durante la era industrial. Todo parece anticipar, por lo tanto, polarizaciones y fragmentaciones territoriales todavía más acentuadas que las conocidas en un pasado cercano. Tomando como referencia el caso de Caracas, se trata de averiguar en qué medida el desarrollo reciente de esta metrópoli sigue las grandes tendencias territoriales antes anotadas; y qué esfuerzos se están haciendo para ajustar sus instancias locales a las nuevas realidades urbanas. Como un recurso adicional, en distintos momentos se establecen comparaciones con Barcelona (España, considerada un ejemplo emblemático de las metrópolis de la era de la información

Removal of Uranium in Drinking Water: Brimac Environmental Services, Inc. Brimac HA 216 Adsorptive Media

Science.gov (United States)

The Brimac HA 216 Adsorptive Media was tested for uranium (U) removal from a drinking water source (well water) at Grappone Toyota located in Bow, New Hampshire. The HA 216 media is a hydroxyapatite-based material. A pilot unit, consisting of a TIGG Corporation Cansorb® C-5 ste...

24 CFR 964.135 - Resident involvement in HA management operations.

Science.gov (United States)

2010-04-01

... management operations. 964.135 Section 964.135 Housing and Urban Development Regulations Relating to Housing... Tenant Participation § 964.135 Resident involvement in HA management operations. Residents shall be... responsibility for management operations, it shall ensure strong resident participation in all issues and facets...

Microstructure, mechanical property and corrosion behavior of interpenetrating (HA + β-TCP)/MgCa composite fabricated by suction casting

International Nuclear Information System (INIS)

Wang, X.; Dong, L.H.; Li, J.T.; Li, X.L.; Ma, X.L.; Zheng, Y.F.

2013-01-01

The novel interpenetrating (HA + β-TCP)/MgCa composites were fabricated by infiltrating MgCa alloy into porous HA + β-TCP using suction casting technique. The microstructure, mechanical properties and corrosion behaviors of the composites have been evaluated by means of scanning electron microscopy (SEM), X-ray diffraction (XRD), mechanical testing, electrochemical and immersion tests. It was shown that the composites had compact structure and the interfacial bonding between MgCa alloy and HA + β-TCP scaffolds was very well. The ultimate compressive strength of the composites was about 500–1000 fold higher than that of the original porous scaffolds, and it still retained quarter-half of the strength of the bulk MgCa alloy. The electrochemical and immersion tests indicated that the corrosion resistance of the composites was better than that of the MgCa matrix alloy, and the corrosion products of the composite surface were mainly Mg(OH) 2 , HA and Ca 3 (PO 4 ) 2 . Meanwhile, the mechanical and corrosive properties of the (HA + β-TCP)/MgCa composites were adjustable by the choice of HA content. - Highlights: • The composites were fabricated by infiltrating MgCa alloy into porous HA + β-TCP. • The microstructure, mechanical and corrosion properties were investigated. • It showed composites had compact structures and good interfacial bonding. • The mechanical and corrosive properties can be adjustable by the HA content. • The corrosion mechanism of the composite has been explained

Description of rainfall variability in Br hat -samhita of Varâha-mihira

OpenAIRE

Iyengar, RN

2004-01-01

Br hat -samhita of Varâha-mihira (5–6th century AD) provides valuable information on the approach in ancient India towards monsoon rainfall, including its measurement and forecasting. In this context, we come across a description of the expected amount of total seasonal rainfall depending on the first rains under the 27 naks atras of Indian astronomy. This provides a rough statistical picture of what might have been the rainfall and its variability in the region around Ujjain, where Varâha-mi...

Synthesis and characterization of praseodymium-142 hydroxyapatite (142Pr-HA)

International Nuclear Information System (INIS)

Duyeh Setiawan; Daud Nurhasan

2015-01-01

The use of radioisotope of lanthanide group with range of beta energy 0.4 - 2.2 MeV has been renewed interest in nuclear medicine. Praseodymium-142 radioisotope ( 142 Pr, t 1/2 = 19.2 hours, E β = 2.16 MeV) is suitable for applications radiotherapy. Labelled of the hydroxyapatite (Ca 10 (PO 4 )6(OH) 2 ) can be used as carrier of radionuclide after injection in the body injection. This research aim as a preliminary studies to make praseodymium-142 hydroxyapatite ( 142 Pr-HA) as a radiotherapy agent. The optimum condition of praseodymium-142 hydroxyapatite synthesis by controlling several parameters such as the pH and the weight of hydroxyapatite was obtained from process by used the nonradioactive praseodymium. The optimum condition of hydroxyapatite by praseodymium are at pH 5 and weight ratio praseodymium : hydroxyapatite is 1 : 16. The percentage of labeling hydroxyapatite with 142 PrCl 3 was 99.50% and the radiochemical purity of 142 Pr-Ha was 95.20%. (author)

Una carta de pésame en arameo de Samuel ha-Naguid

Directory of Open Access Journals (Sweden)

Alba Cecilia, Amparo

2007-12-01

Full Text Available In this article we offer a Spanish translation with a commentary of an Aramaic letter of consolation written by Samuel ha-Naguid and sent to R. Ḥananel b. Ḥušiel, lamenting the death of R. Ḥušiel b. Elḥanán, his father.

En el presente artículo ofrecemos la traducción y el estudio de la carta de pésame enviada por Samuel ha-Naguid a R. Ḥananel b. Ḥušiel por la muerte de R. Ḥušiel b. Elḥanán, su padre.

Genetic alterations in Ki-ras and Ha-ras genes in Juvenile Nasopharyngeal Angiofibromas and head and neck cancer

Directory of Open Access Journals (Sweden)

Cláudia Malheiros Coutinho

1999-05-01

Full Text Available CONETXT: Ras gene mutations have been associated to a wide range of human solid tumors. Members of the ras gene family (Ki-ras, Ha-ras and N-ras are structurally related and code for a protein (p21 known to play an important role in the regulation of normal signal transduction and cell growth. The frequency of ras mutations is different from one type of tumor to another, suggesting that point mutations might be carcinogen-specific. OBJECTIVES: To study the occurrence of Ki-ras and Ha-ras mutations. We also studied the relative level of Ha-ras mRNA in 32 of the head and neck tumors. DESIGN: Case series. SETTING: University referral unit. PARTICIPANTS: 60 head and neck tumors and in 28 Juvenile Nasopharyngeal Angiofibromas (JNA. DIAGNOSTIC TEST: Using PCR-SSCP we examined the occurrence of Ki-ras and Ha-ras mutations. The relative level of Ha-ras mRNA was examined by Northern blot analysis. RESULTS: None of the head and neck tumors or JNA samples showed evidence of mutations within codons 12, 13, 59 and 61 of Ki-ras or Ha-ras genes. However, 17 (53% of the tumors where gene expression could be examined exhibited increased levels of Ha-ras mRNA compared with the normal tissue derived from the same patient. CONCLUSIONS: Our results demonstrate for the first time that mutations of Ki-ras and Ha-ras genes are not associated with the development of JNA and confirm previous reports indicating that activating ras mutations are absent or rarely involved in head and neck tumors from western world patients. Furthermore, our findings suggest that overexpression of Ha-ras, rather than mutations, might be an important factor in the development and progression of head and neck tumors.

An in vivo evaluation of PLLA/PLLA-gHA nano-composite for internal fixation of mandibular bone fractures.

Science.gov (United States)

Peng, Weihai; Zheng, Wei; Shi, Kai; Wang, Wangshu; Shao, Ying; Zhang, Duo

2015-11-09

Internal fixation of bone fractures using biodegradable poly(L-lactic-acid) (PLLA)-based materials has attracted the attention of many researchers. In the present study, 36 male beagle dogs were randomly assigned to two groups: PLLA/PLLA-gHA (PLLA-grafted hydroxyapatite) group and PLLA group. PLLA/PLLA-gHA and PLLA plates were embedded in the muscular bags of the erector spinae and also implanted to fix mandibular bone fractures in respective groups. At 1, 2, 3, 6, 9, and 12 months postoperatively, the PLLA/PLLA-gHA and PLLA plates were evaluated by adsorption and degradation tests, and the mandibles were examined through radiographic analysis, biomechanical testing, and histological analysis. The PLLA/PLLA-gHA plates were non-transparent and showed a creamy white color, and the PLLA plates were transparent and faint yellow in color. At all time points following surgery, adsorption and degradation of the PLLA/PLLA-gHA plates were significantly less than those of the PLLA plates, and the lateral and longitudinal bending strengths of the surgically treated mandibles of the beagle dogs in the PLLA/PLLA-gHA group were significantly greater than those of the PLLA group and reached almost the value of intact mandibles at 12 months postoperatively. Additionally, relatively rapid bone healing was observed in the PLLA/PLLA-gHA group with the formation of new lamellar bone tissues at 12 months after the surgery. The PLLA/PLLA-gHA nano-composite can be employed as a biodegradable material for internal fixation of mandibular bone fractures.

An in vivo evaluation of PLLA/PLLA-gHA nano-composite for internal fixation of mandibular bone fractures

International Nuclear Information System (INIS)

Peng, Weihai; Shi, Kai; Wang, Wangshu; Shao, Ying; Zhang, Duo; Zheng, Wei

2015-01-01

Internal fixation of bone fractures using biodegradable poly(L-lactic-acid) (PLLA)-based materials has attracted the attention of many researchers. In the present study, 36 male beagle dogs were randomly assigned to two groups: PLLA/PLLA-gHA (PLLA-grafted hydroxyapatite) group and PLLA group. PLLA/PLLA-gHA and PLLA plates were embedded in the muscular bags of the erector spinae and also implanted to fix mandibular bone fractures in respective groups. At 1, 2, 3, 6, 9, and 12 months postoperatively, the PLLA/PLLA-gHA and PLLA plates were evaluated by adsorption and degradation tests, and the mandibles were examined through radiographic analysis, biomechanical testing, and histological analysis. The PLLA/PLLA-gHA plates were non-transparent and showed a creamy white color, and the PLLA plates were transparent and faint yellow in color. At all time points following surgery, adsorption and degradation of the PLLA/PLLA-gHA plates were significantly less than those of the PLLA plates, and the lateral and longitudinal bending strengths of the surgically treated mandibles of the beagle dogs in the PLLA/PLLA-gHA group were significantly greater than those of the PLLA group and reached almost the value of intact mandibles at 12 months postoperatively. Additionally, relatively rapid bone healing was observed in the PLLA/PLLA-gHA group with the formation of new lamellar bone tissues at 12 months after the surgery. The PLLA/PLLA-gHA nano-composite can be employed as a biodegradable material for internal fixation of mandibular bone fractures. (paper)

Laminated electrospun nHA/PHB-composite scaffolds mimicking bone extracellular matrix for bone tissue engineering

Energy Technology Data Exchange (ETDEWEB)

Chen, Zhuoyue [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Song, Yue [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Zhang, Jing [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Ministry of Education, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province, 710069 (China); Liu, Wei [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Cui, Jihong, E-mail: cjh@nwu.edu.cn [Lab of Tissue Engineering, Faculty of Life Science, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province 710069 (China); Key Laboratory of Resource Biology and Modern Biotechnology in Western China, Ministry of Education, Northwest University, 229 TaiBai North Road, Xi' an, Shaanxi Province, 710069 (China); and others

2017-03-01

Electrospinning is an effective means to generate nano- to micro-scale polymer fibers resembling native extracellular matrix for tissue engineering. However, a major problem of electrospun materials is that limited pore size and porosity may prevent adequate cellular infiltration and tissue ingrowth. In this study, we first prepared thin layers of hydroxyapatite nanoparticle (nHA)/poly-hydroxybutyrate (PHB) via electrospinning. We then laminated the nHA/PHB thin layers to obtain a scaffold for cell seeding and bone tissue engineering. The results demonstrated that the laminated scaffold possessed optimized cell-loading capacity. Bone marrow mesenchymal stem cells (MSCs) exhibited better adherence, proliferation and osteogenic phenotypes on nHA/PHB scaffolds than on PHB scaffolds. Thereafter, we seeded MSCs onto nHA/PHB scaffolds to fabricate bone grafts. Histological observation showed osteoid tissue formation throughout the scaffold, with most of the scaffold absorbed in the specimens 2 months after implantation, and blood vessels ingrowth into the graft could be observed in the graft. We concluded that electrospun and laminated nanoscaled biocomposite scaffolds hold great therapeutic potential for bone regeneration. - Highlights: • We laminated the nHA/PHB layers to obtain a scaffold for bone tissue engineering. • The laminated scaffold performed optimized cell-loading capacity. • MSCs exhibited osteogenic phenotypes on the laminated scaffold. • Osteoid tissue formed throughout the laminated scaffold after 2 months in vivo. The laminated bio-composite scaffolds can be applied to bone regeneration.

Laminated electrospun nHA/PHB-composite scaffolds mimicking bone extracellular matrix for bone tissue engineering

International Nuclear Information System (INIS)

Chen, Zhuoyue; Song, Yue; Zhang, Jing; Liu, Wei; Cui, Jihong

2017-01-01

Electrospinning is an effective means to generate nano- to micro-scale polymer fibers resembling native extracellular matrix for tissue engineering. However, a major problem of electrospun materials is that limited pore size and porosity may prevent adequate cellular infiltration and tissue ingrowth. In this study, we first prepared thin layers of hydroxyapatite nanoparticle (nHA)/poly-hydroxybutyrate (PHB) via electrospinning. We then laminated the nHA/PHB thin layers to obtain a scaffold for cell seeding and bone tissue engineering. The results demonstrated that the laminated scaffold possessed optimized cell-loading capacity. Bone marrow mesenchymal stem cells (MSCs) exhibited better adherence, proliferation and osteogenic phenotypes on nHA/PHB scaffolds than on PHB scaffolds. Thereafter, we seeded MSCs onto nHA/PHB scaffolds to fabricate bone grafts. Histological observation showed osteoid tissue formation throughout the scaffold, with most of the scaffold absorbed in the specimens 2 months after implantation, and blood vessels ingrowth into the graft could be observed in the graft. We concluded that electrospun and laminated nanoscaled biocomposite scaffolds hold great therapeutic potential for bone regeneration. - Highlights: • We laminated the nHA/PHB layers to obtain a scaffold for bone tissue engineering. • The laminated scaffold performed optimized cell-loading capacity. • MSCs exhibited osteogenic phenotypes on the laminated scaffold. • Osteoid tissue formed throughout the laminated scaffold after 2 months in vivo. The laminated bio-composite scaffolds can be applied to bone regeneration.

Evaluación y acreditación del profesorado, programas e instituciones educativas

Directory of Open Access Journals (Sweden)

Fuensanta Hernández Pina

2014-01-01

Full Text Available La implantación del Proceso de Bolonia para el desarrollo del Espacio Europeo de la Educación Superior (EEES ha supuesto importantes cambios estructurales y de fondo en las universidades españolas y, como consecuencia, en todo el sistema educativo español. En paralelo a estos cambios el nivel universitario se ha dotado de un sistema que garantice su calidad y su mejora continua a partir de procesos de orientación, evaluación, certificación y acreditación. Es decir, de un sistema de aseguramiento de la calidad que garantice la promoción y la calidad de las instituciones. Para el cumplimiento de la evaluación, la certificación y la acreditación, la ANECA creada en 2002, como fundación estatal, tiene como objetivo “aportar garantía externa de calidad al sistema universitario y contribuir a su mejora constante” (ANECA, 2013. Para llevar a cabo su actividad evaluadora, de certificación y de acreditación, ANECA ha puesto en marcha varios programas que cubren todo el espectro: las enseñanzas con programas como VERIFICA, MONITOR, ACREDITA y ACREDITA-Plus y MENCIÓN, la institución con el programa AUDIT y el profesorado con los programas PEP y ACADEMIA. Todos estos programas de forma integrada tratan de garantizar la calidad de la formación de sus estudiantes, la calidad de los que los imparten dichos programas y la calidad de las instituciones donde tienen lugar los programas formativos.

An interpenetrating HA/G/CS biomimic hydrogel via Diels-Alder click chemistry for cartilage tissue engineering.

Science.gov (United States)

Yu, Feng; Cao, Xiaodong; Zeng, Lei; Zhang, Qing; Chen, Xiaofeng

2013-08-14

In order to mimic the natural cartilage extracellular matrix, a novel biological degradable interpenetrating network hydrogel was synthesized from the gelatin (G), hyaluronic acid (HA) and chondroitin sulfate (CS) by Diels-Alder "click" chemistry. HA was modified with furylamine and G was modified with furancarboxylic acid respectively. (1)H NMR spectra and elemental analysis showed that the substitution degrees of HA-furan and G-furan were 71.5% and 44.5%. Then the hydrogels were finally synthesized by cross-linking furan-modified HA and G derivatives with dimaleimide poly(ethylene glycol) (MAL-PEG-MAL). The mechanical and degradation properties of the hydrogels could be tuned simply through varying the molar ratio between furan and maleimide. Rheological, mechanical and degradation studies demonstrated that the Diels-Alder "click" chemistry is an efficient method for preparing high performance biological interpenetrating hydrogels. This biomimic hydrogel with improved mechanical properties could have great potential applications in cartilage tissue engineering. Copyright © 2013 Elsevier Ltd. All rights reserved.

Powder injection molding of HA/Ti6Al4V composite using palm stearin as based binder for implant material

International Nuclear Information System (INIS)

Arifin, Amir; Sulong, Abu Bakar; Muhamad, Norhamidi; Syarif, Junaidi; Ramli, Mohd Ikram

2015-01-01

Highlights: • Fabrication of HA/Ti6Al4V composite using powder injection molding. • Rheological results show that palm stearin is suitable as binder. • Resulted mechanical properties in between titanium alloy and HA values. • Micro porous enable accelerated bioactivity based on in vitro test. - Abstract: Titanium alloy (Ti6Al4V) and hydroxyapatite (HA) are well-known materials applied in implants. Ti6Al4V shows good mechanical properties and corrosion resistance, whereas HA possesses excellent biocompatibility and bioactivity but weak mechanical properties. The combination of the Ti6Al4V and HA properties is expected to produce a superior material for bio-implants. This study aimed to analyze the feasibility of fabricating HA/Ti6Al4V composites through powder injection molding (PIM) using palm stearin as base binder. In this study, 90 wt% Ti6Al4V and 10 wt% HA were mixed with the palm stearin and polyethylene binder system. The HA/Ti6Al4V feedstock showed pseudoplastic properties, suggesting its suitability for PIM. Flexural test revealed that the strength of the sintered composite ranges from 67.12 MPa to 112.97 MPa and its Young’s modulus ranges from 39.28 GPa to 44.25 GPa. The X-ray diffraction patterns and energy-dispersive X-ray spectra of the composite showed that the HA decomposed and formed secondary phases. Isotropic porous structure was observed on the sintered sample because of HA decomposition. Results showed that the palm stearin can be used as based binder in fabricating HA/Ti6Al4V composites via PIM. The mechanical properties of the sintered composites are nearly similar to those of the human bone. In addition, the increase in weight of the sintered composite during in vitro tests indicated the nucleation and growth of the Ca–P phase, which exhibited the biocompatibility of the fabricated HA/Ti6Al4V composite

Programa RADAR en Iberdrola: "Incremento del compromiso personal/grupal/organizacional para conseguir el éxito en prevención de riesgos laborales": experiencia RADAR 2009-2013

Directory of Open Access Journals (Sweden)

Antonio Moreno Ucelay

2013-01-01

Full Text Available Una vez alcanzada una madurez en IBERDROLA, en el ámbito de la prevención de riesgos laborales, dado que se ha llegado a niveles importantes de información, formación, certificaciones, inspecciones, comités, etc., con una accidentalidad reducida, se ha buscado ir más lejos generando un mayor compromiso en el liderazgo de: - El empleado en la gestión segura de las actividades que realizan individualmente - Su grupo de pertenencia - Las Organizaciones que componen la empresa. La aplicación del Programa Radar en las empresas de IBERDROLA Grupo ha sido fundamental para generar un cambio cultural en el ámbito de la prevención en los diferentes colectivos a los que se dirige así como en el aumento de motivación y compromiso personal, especialmente de las personas que más oportunidad tienen de intervenir, los propios trabajadores. A nivel práctico se ha observado que ha provocado mejoras más allá del ámbito de la prevención de riesgos laborales como la mejora en la autoestima del trabajador, incremento de la comunicación con estamentos al mismo nivel, de nivel superior, con el Servicio de Prevención y con organizaciones especializadas, también se ha incrementado el sentido de pertenencia al equipo y la orientación a resultados de los grupos que han intervenido. Los positivos resultados obtenidos han hecho que esta iniciativa se expanda, fuera del ámbito español, a otras empresas del grupo tanto en Europa como en América.

Working and Giving Birth in the United States: Changing Strategies of Transborder Life in the North of Mexico

Directory of Open Access Journals (Sweden)

EUNICE D. VARGAS VALLE

2017-01-01

Full Text Available Este estudio analiza los cambios en dos procesos transfronterizos: el empleo y dar a luz en es- tados u nidos, entre las poblaciones de la frontera norte de m éxico entre 2000 y 2010. s e utilizan diversos métodos estadísticos para analizar las tendencias y cambios en los perf iles socioeconómicos de quienes despliegan estas prácticas. a la par que se registró un descenso en los trabajadores transfronterizos, los nacimientos en estados u nidos aumentaron y ocurrió una mayor concentración de ambas prácticas en poblaciones con altos niveles educativos. e stos resultados sugieren que esta frontera ha llegado a ser una barrera más selectiva para las interac - ciones transfronterizas.

Antología de la poesía Eslovena Traducción y notas de María Francisca. de Castro Gil, Introducción de France Koren, Madrid, 1954

Directory of Open Access Journals (Sweden)

Irene Mislej

1995-12-01

Full Text Available Por intennedio de un distinguido cientifico es1oveno que reside en Canadá, el dr. Srečko Pregelj, ha llegado a mis manos un manuscrito sumamente interesante. Se trata de una Antología de la Poesía eslovena, la cual evidentemente había sido preparada para su impresión en 1954, en Madrid. El hecho de que contemos con tan pocas traducciones de obras literarias eslovenas al castellano hace de este descubrimiento algo realmente curioso e importante. La obra es fruto de la colaboración estrecha de un emigrante esloveno, France Koren y de una escritora y poetisa espanola, María Francisca de Castro Gil. El nacimiento de una obra tan singular merece algunas palabras de explicación.

Rights of the child in Salesian code

Directory of Open Access Journals (Sweden)

José Luis Guzón

2010-12-01

Full Text Available SíntesisEl autor comienza su artículo con un recorrido histórico para entender cómo se ha llegado al concepto de ‘menor’. Inmediatamente después deestas primeras reflexiones afirma que la educación es un derecho fundamental reconocido universalmente. Desde esta clave sitúa la labor pastoral del joven sacerdote Juan Bosco, quien se acerca al mundo juvenil con una finalidad educativo-evangelizadora. La educación sigue siendo –afirma el autor- el principal problema de nuestra sociedad,y hoy somos testigos de algunas dificultades en el ámbito educativo: crisis de autoridad, de sentido y de socialización. En la última parte del artículo centra sus reflexiones en el Sistema Preventivo como respuesta a la urgencia educativa.

Evolución de la inmigración en España 1998- 2014, aportes y el reto a futuro.

Directory of Open Access Journals (Sweden)

Ana del Pilar Banda Mora

2015-04-01

Full Text Available En este estudio se busca demostrar que España necesitará de la inmigración prontamente. La crisis económica española actual ha trastocado la dinámica inmigratoria, una vez superado este hecho, dicho fenómeno seguirá en ascenso ante el envejecimiento poblacional crónico y la baja tasa de natalidad para no poner en riesgo el anhelado crecimiento y desarrollo económico, de ahí que las políticas de inmigración deben tener un planeamiento mesurado, técnico y no ser simplemente producto de improvisaciones, como en el pasado reciente, donde no se supo aprovechar, de manera adecuada, la riqueza de capital humano llegado a la península ibérica

Marco conceptual para la evaluación de programas de salud

Directory of Open Access Journals (Sweden)

Montero Rojas, Eiliana

2004-03-01

Full Text Available La evaluación como disciplina es un área relativamente nueva de las Ciencias Sociales; consecuentemente, su incorporación explícita en los programas y sistemas de salud ha llegado algo tardíamente. Este artículo presenta elementos de un marco conceptual para concebir y utilizar la evaluación en programas de salud. Se define la evaluación como una herramienta para la toma de decisiones, se discuten tendencias y temáticas de actualidad en el campo y también conceptos clave tales como teoría del programa y valoración de necesidades. Finalmente, se esboza una primera aproximación a dos constructos de gran interés en la evaluación de programas de salud: calidad y equidad.

Laminated electrospun nHA/PHB-composite scaffolds mimicking bone extracellular matrix for bone tissue engineering.

Science.gov (United States)

Chen, Zhuoyue; Song, Yue; Zhang, Jing; Liu, Wei; Cui, Jihong; Li, Hongmin; Chen, Fulin

2017-03-01

Electrospinning is an effective means to generate nano- to micro-scale polymer fibers resembling native extracellular matrix for tissue engineering. However, a major problem of electrospun materials is that limited pore size and porosity may prevent adequate cellular infiltration and tissue ingrowth. In this study, we first prepared thin layers of hydroxyapatite nanoparticle (nHA)/poly-hydroxybutyrate (PHB) via electrospinning. We then laminated the nHA/PHB thin layers to obtain a scaffold for cell seeding and bone tissue engineering. The results demonstrated that the laminated scaffold possessed optimized cell-loading capacity. Bone marrow mesenchymal stem cells (MSCs) exhibited better adherence, proliferation and osteogenic phenotypes on nHA/PHB scaffolds than on PHB scaffolds. Thereafter, we seeded MSCs onto nHA/PHB scaffolds to fabricate bone grafts. Histological observation showed osteoid tissue formation throughout the scaffold, with most of the scaffold absorbed in the specimens 2months after implantation, and blood vessels ingrowth into the graft could be observed in the graft. We concluded that electrospun and laminated nanoscaled biocomposite scaffolds hold great therapeutic potential for bone regeneration. Copyright © 2016 Elsevier B.V. All rights reserved.

Actividades emprendedoras y espacios alternativos de aprendizaje. Desarrollo de competencias durante un hackathon day

Directory of Open Access Journals (Sweden)

Carmen Ruiz Viñals

2017-05-01

Full Text Available El proyecto de los espacios sociales de aprendizaje en la Universidad Española se inició como parte del cambio de modelo educativo propuesto por el proceso de Bolonia. Dichos espacios los conforman todos los lugares que no son los aularios y que ayudan a completar el aprendizaje formal. En la presente investigación se reflexiona sobre estos espacios y las actividades que se desarrollan en ellos, donde los estudiantes adquieren las competencias y habilidades más demandadas por el mercado laboral, especialmente las llamadas competencias emprendedoras. Anualmente se organiza un maratón de emprendimiento llamado Barcelona Thinking Challenge en la UAO CEU. Dicha actividad es un espacio de aprendizaje donde los estudiantes adquieren competencias transversales y específicas, trabajando en equipos formados por estudiantes de diferentes cursos, universidades y profesionales. Se analizan los resultados de un estudio de encuesta que valora cuál ha sido la visión general de este maratón de emprendimiento social y qué competencias emprendedoras ha permitido desarrollar en los participantes. Los resultados permiten destacar que los partícipes valoran positivamente esta actividad emprendedora. Se muestra un alto desarrollo en las competencias relacionadas con trabajar en equipo, liderazgo y aplicación de conocimientos adquiridos en la universidad a la resolución de problemas.

Transgenic Cotton Plants Expressing the HaHR3 Gene Conferred Enhanced Resistance to Helicoverpa armigera and Improved Cotton Yield.

Science.gov (United States)

Han, Qiang; Wang, Zhenzhen; He, Yunxin; Xiong, Yehui; Lv, Shun; Li, Shupeng; Zhang, Zhigang; Qiu, Dewen; Zeng, Hongmei

2017-08-30

RNA interference (RNAi) has been developed as an efficient technology. RNAi insect-resistant transgenic plants expressing double-stranded RNA (dsRNA) that is ingested into insects to silence target genes can affect the viability of these pests or even lead to their death. HaHR3 , a molt-regulating transcription factor gene, was previously selected as a target expressed in bacteria and tobacco plants to control Helicoverpa armigera by RNAi technology. In this work, we selected the dsRNA- HaHR3 fragment to silence HaHR3 in cotton bollworm for plant mediated-RNAi research. A total of 19 transgenic cotton lines expressing HaHR3 were successfully cultivated, and seven generated lines were used to perform feeding bioassays. Transgenic cotton plants expressing ds HaHR3 were shown to induce high larval mortality and deformities of pupation and adult eclosion when used to feed the newly hatched larvae, and 3rd and 5th instar larvae of H. armigera . Moreover, HaHR3 transgenic cotton also demonstrated an improved cotton yield when compared with controls.

Hyaluronic acid doped-poly(3,4-ethylenedioxythiophene)/chitosan/gelatin (PEDOT-HA/Cs/Gel) porous conductive scaffold for nerve regeneration

International Nuclear Information System (INIS)

Wang, Shuping; Guan, Shui; Zhu, Zhibo; Li, Wenfang; Liu, Tianqing; Ma, Xuehu

2017-01-01

Conducting polymer, as a “smart” biomaterial, has been increasingly used to construct tissue engineered scaffold for nerve tissue regeneration. In this study, a novel porous conductive scaffold was prepared by incorporating conductive hyaluronic acid (HA) doped-poly(3,4-ethylenedioxythiophene) (PEDOT-HA) nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. The physicochemical characteristics of Cs/Gel scaffold with 0–10 wt% PEDOT-HA were analyzed and the results indicated that the incorporation of PEDOT-HA into scaffold increased the electrical and mechanical properties while decreasing the porosity and water absorption. Moreover, in vitro biodegradation of scaffold displayed a declining trend with the PEDOT-HA content increased. About the biocompatibility of conductive scaffold, neuron-like rat phaeochromocytoma (PC12) cells were cultured in scaffold to evaluate cell adhesion and growth. 8% PEDOT-HA/Cs/Gel scaffold had a higher cell adhesive efficiency and cell viability than the other conductive scaffolds. Furthermore, cells in the scaffold with 8 wt% PEDOT-HA expressed higher synapse growth gene of GAP43 and SYP compared with Cs/Gel control group. These results suggest that 8%PEDOT-HA/Cs/Gel scaffold is an attractive cell culture conductive substrate which could support cell adhesion, survival, proliferation, and synapse growth for the application in nerve tissue regeneration. - Highlights: • A porous conductive scaffold was prepared by freeze-drying method. • Conductive scaffold could support PC12 cells adhesion, survival, and proliferation. • Cells in conductive scaffold expressed high synapse growth gene of GAP43 and SYP.

Hyaluronic acid doped-poly(3,4-ethylenedioxythiophene)/chitosan/gelatin (PEDOT-HA/Cs/Gel) porous conductive scaffold for nerve regeneration

Energy Technology Data Exchange (ETDEWEB)

Wang, Shuping; Guan, Shui, E-mail: guanshui@dlut.edu.cn; Zhu, Zhibo; Li, Wenfang; Liu, Tianqing; Ma, Xuehu

2017-02-01

Conducting polymer, as a “smart” biomaterial, has been increasingly used to construct tissue engineered scaffold for nerve tissue regeneration. In this study, a novel porous conductive scaffold was prepared by incorporating conductive hyaluronic acid (HA) doped-poly(3,4-ethylenedioxythiophene) (PEDOT-HA) nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. The physicochemical characteristics of Cs/Gel scaffold with 0–10 wt% PEDOT-HA were analyzed and the results indicated that the incorporation of PEDOT-HA into scaffold increased the electrical and mechanical properties while decreasing the porosity and water absorption. Moreover, in vitro biodegradation of scaffold displayed a declining trend with the PEDOT-HA content increased. About the biocompatibility of conductive scaffold, neuron-like rat phaeochromocytoma (PC12) cells were cultured in scaffold to evaluate cell adhesion and growth. 8% PEDOT-HA/Cs/Gel scaffold had a higher cell adhesive efficiency and cell viability than the other conductive scaffolds. Furthermore, cells in the scaffold with 8 wt% PEDOT-HA expressed higher synapse growth gene of GAP43 and SYP compared with Cs/Gel control group. These results suggest that 8%PEDOT-HA/Cs/Gel scaffold is an attractive cell culture conductive substrate which could support cell adhesion, survival, proliferation, and synapse growth for the application in nerve tissue regeneration. - Highlights: • A porous conductive scaffold was prepared by freeze-drying method. • Conductive scaffold could support PC12 cells adhesion, survival, and proliferation. • Cells in conductive scaffold expressed high synapse growth gene of GAP43 and SYP.

Silencing of HaAce1 gene by host-delivered artificial microRNA disrupts growth and development of Helicoverpa armigera.

Science.gov (United States)

Saini, Ravi Prakash; Raman, Venkat; Dhandapani, Gurusamy; Malhotra, Era Vaidya; Sreevathsa, Rohini; Kumar, Polumetla Ananda; Sharma, Tilak R; Pattanayak, Debasis

2018-01-01

The polyphagous insect-pest, Helicoverpa armigera, is a serious threat to a number of economically important crops. Chemical application and/or cultivation of Bt transgenic crops are the two strategies available now for insect-pest management. However, environmental pollution and long-term sustainability are major concerns against these two options. RNAi is now considered as a promising technology to complement Bt to tackle insect-pests menace. In this study, we report host-delivered silencing of HaAce1 gene, encoding the predominant isoform of H. armigera acetylcholinesterase, by an artificial microRNA, HaAce1-amiR1. Arabidopsis pre-miRNA164b was modified by replacing miR164b/miR164b* sequences with HaAce1-amiR1/HaAce1-amiR1* sequences. The recombinant HaAce1-preamiRNA1 was put under the control of CaMV 35S promoter and NOS terminator of plant binary vector pBI121, and the resultant vector cassette was used for tobacco transformation. Two transgenic tobacco lines expressing HaAce1-amiR1 was used for detached leaf insect feeding bioassays. Larval mortality of 25% and adult deformity of 20% were observed in transgenic treated insect group over that control tobacco treated insect group. The reduction in the steady-state level of HaAce1 mRNA was 70-80% in the defective adults compared to control. Our results demonstrate promise for host-delivered amiRNA-mediated silencing of HaAce1 gene for H. armigera management.

Clinical significance of determination of changes of serum HA, IL-2 and TNF-α contents in patients with psoriasis

International Nuclear Information System (INIS)

Ren Hong

2006-01-01

Objective: To investigate the changes of serum HA, IL-2 and TNF-α contents in patients with psoriasis. Methods: Serum HA, TNF-α levels were measured with RIA and IL-2 levels was measured with ELISA in 47 patients with psoriasis as well as 35 controls. Results: The serum HA, TNF-α levels were significantly higher in the patients than those in controls (P < 0. 01), while the serum IL-2 levels were significantly lower (P<0.01). Conclusion: Determination of serum HA, IL-2 and TNF-α contents would be clinically useful for understanding the disturbances of immunomodulation in these patients. (authors)

pH-Amplified multilayer films based on hyaluronan: influence of HA molecular weight and concentration on film growth and stability.

Science.gov (United States)

Shen, Liyan; Chaudouet, Patrick; Ji, Jian; Picart, Catherine

2011-04-11

In this study, we investigate the growth and internal properties of polyelectrolyte multilayer films made of poly(l-lysine) and hyaluronan (PLL/HA) under pH-amplified conditions, that is, by alternate deposition of PLL at high pH and HA at low pH. We focus especially on the influence of the molecular weight of HA in this process as well as on its concentration in solution. Film growth was followed by quartz crystal microbalance and by infrared spectroscopy to quantify the deposited mass and to characterize the internal properties of the films, including the presence of hydrogen bonds and the ionization degree of HA in the films. Film growth was significantly faster for HA of high molecular weight (1300 kDa) as compared with 400 and 200 kDa. PLL was found to exhibit a random structure once deposited in the films. Furthermore, we found that PLL-ending films are more stable when they are placed in PBS than their HA counterparts. This was explained on the basis of more cohesive interactions in the films for PLL-ending films. Finally, we quantified PLL(FITC) diffusion into the films and observed that PLL diffusion is enhanced when PLL is paired with the HA of high MW. All together, these results suggest that besides purely physicochemical parameters such as variation in pH, the molecular weight of HA, its concentration in solution, and the possibility to form intermolecular HA association play important roles in film growth, internal cohesion, and stability.

Laser fabrication of Ag-HA nanocomposites on Ti6Al4V implant for enhancing bioactivity and antibacterial capability

Energy Technology Data Exchange (ETDEWEB)

Liu, Xiangmei; Man, H.C., E-mail: mfhcman@polyu.edu.hk

2017-01-01

For titanium alloy implants, both surface bioactivity and antibacterial infection are the two critical factors in determining the success of clinical implantation of these metallic implants. In the present work, a novel nanocomposite layer of nano-silver-containing hydroxyapatite (Ag-HA) was prepared on the surface of biomedical Ti6Al4V by laser processing. Analysis using SEM, EDS and XRD shows the formation of an Ag-HA layer of about 200 μm fusion bonded to the substrate. Mineralization tests in simulated body fluid (SBF) showed that laser fabricated Ag-HA nanocomposite layer favors the deposition of apatite on the surface of the implants. Antibacterial tests confirmed that all Ag-HA nanocomposite layers can kill bacteria while a higher Ag content would lower the cytocompatibility of these coatings. Cell viability decreases when the Ag content reaches 5% in these coatings, due to the larger amount of Ag leached out, as confirmed by ion release evaluation. Our results reveal that laser fabricated Ag-HA nanocomposite coatings containing 2% Ag show both excellent cytocompatibility and antibacterial capability. - Highlights: • Silver-containing hydroxyapatite (Ag-HA) nanocomposite layer was fabricated on Ti6Al4V by laser technique. • Both bioactivity and antibacterial capability were significantly enhanced compared with bare Ti6Al4V. • Ag-HA nanocomposite coatings containing 2% Ag show both excellent cytocompatibility and antibacterial capability.

Effects of DCPD cement chemistry on degradation properties and cytocompatibility: comparison of MCPM/β-TCP and MCPM/HA formulations

International Nuclear Information System (INIS)

Alge, Daniel L; Goebel, W Scott; Chu, Tien-Min Gabriel

2013-01-01

Dicalcium phosphate dihydrate (DCPD) cements are attractive biomaterials for bone repair, and a number of different DCPD cement formulations have been proposed in the literature. In this study, we have specifically compared monocalcium phosphate monohydrate (MCPM)/hydroxyapatite (HA) and MCPM/β-tricalcium phosphate (β-TCP) formulations to test the hypothesis that DCPD cement chemistry affects the degradation properties and cytocompatibility of the cement. Using simple in vitro models we found that MCPM/β-TCP formulations degraded primarily by DCPD dissolution, which was associated with a slight pH drop and relatively low mass loss. Cytocompatibility testing of cement conditioned culture media revealed no significant change in cell viability relative to the negative control for all of the MCPM/β-TCP formulations. In contrast, the MCPM/HA formulations were prone to undergo rapid conversion of DCPD to HA, resulting in a sharp pH drop and extensive mass loss. A stoichiometric excess of HA in the cement was found to accelerate the conversion process, and significant cytotoxicity was observed for the MCPM/HA formulations containing excess HA. Collectively, these results show that, although the product of the setting reaction is the same, DCPD cements produced with MCPM/HA and MCPM/β-TCP formulations differ significantly in their degradation properties and cytocompatibility. These differences may have important implications for the selection of a DCPD cement formulation for clinical application. (paper)

Production of polyclonal antibody against Tehran strain influenza virus (A/H1N1/2009 hemagglutinin conserved domain (HA2: brief report

Directory of Open Access Journals (Sweden)

Somayeh Zamani

2015-10-01

Full Text Available Background: The influenza virus is one of the most important factors for higher morbidity and mortality in the world. Recently, researchers have been focused on influenza conserved antigenic proteins such as hemagglutinin stalk domain (HA2 for vaccine production and serological studies. The HA2 plays a major role in the fusion of the virus with host cells membrane. The immunity system enables to produce antibody against HA2. The aim of this study is polyclonal antibody production against influenza HA2. Methods: This study was done in the Influenza Research Lab, Pasteur Institute of Iran, Tehran for one year from September 2013 to October 2014. In the present study, recombinant HA2 protein was produced in prokaryotic system and purified using Nickel affinity chromatography. The purified HA2 was mixed with Freund’s adjuvant (complete and incomplete and injected into two New Zealand white rabbits by intramuscularly and subcutaneously routes. Immunization was continued for several months with two weeks interval. Before each immunization, blood was drawn by venous puncture from the rabbit ear. Function of rabbit's sera was evaluated using radial immunodiffusion (RID in both forms, Single RID (SRID and Double RID (DRID. Finally, antiserum activity against HA2 was evaluated using western blotting as serological assay. Results: Sedimentary line and zone was observed in RID assays (SRID and DRID represent interaction between HA2 protein and anti- HA2 antibody. As well as, western blotting results was positive for HA2 protein. Therefore, these results showed that polyclonal antibody produced against HA2 protein can identify HA2 protein antigenic sites. Conclusion: These findings show that humoral immune responses have properly been stimulated in rabbits and these antibodies can identify HA2 protein and may be suitable for other serological methods.

Sääste hoitakse üha enam valuutas / Piret Reiljan

Index Scriptorium Estoniae

Reiljan, Piret, 1983-

2007-01-01

Ilmunud ka: Delovõje Vedomosti 18. juuli lk. 3. Üha enam eraisikuid on oma tähtajalisi ja säästuhoiuseid paigutanud valuutasse, kuna välisraha puhul on valuutarisk väiksem. Kommenteerivad Mart Sõrg, Sven Meimer. Diagramm: Valuutahoiuste maht tõusuteel; Üle aasta kestvatest eraisikute hoiustest 75% valuutas. Tabel: Kroonihoius intressi poolest kuldne kesktee. Lisa: Number

Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells

Science.gov (United States)

Quan, Juan-Hua; Choi, In-Wook; Yang, Jung-Bo; Zhou, Wei; Cha, Guang-Ho; Zhou, Yu; Ryu, Jae-Sook

2014-01-01

Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time- and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite. PMID:25548410

24 CFR 964.18 - HA role in activities under subparts B & C.

Science.gov (United States)

2010-04-01

... encourage the formation of joint HA management-resident committees to work on issues and planning. (9) The... elements of their partnership agreement and it shall be updated at least once every three (3) years. (11...

UTILIZACIÓN DE SOFTWARE DE CORRECCIÓN AUTOMÁTICA EN EL CAMPO DE LAS CIENCIAS DE LA SALUD

Directory of Open Access Journals (Sweden)

Ferrán Prados

2010-07-01

Full Text Available Estamos viviendo una época de cambios profundos en la educación universitaria. La implantación del plan de Bolonia nos ha llevado a plantear nuevas metodologías docentes, a revisar el papel del estudiante, la evaluación por competencias, la incorporación de las TIC. Hechos impensables hace poco más de una década. Entre las diferentes plataformas informáticas, cabe destacar las que permiten corrección automática de ejercicios, porque son instrumentos de un gran interés pedagógico ya que evalúan al instante al alumnado y aportan un feedback del conocimiento que tiene en forma de mensaje de ayuda o de nota. Si la potencia de estas herramientas la sumamos a la de Internet, usando un entorno de e-learning, el resultado permitirá trabajar, corregir, evaluar, resolver dudas, etc., desde cualquier lugar y a cualquier hora. Este trabajo presenta parte de una plataforma y los resultados de su utilización en el ámbito de las ciencias de la salud.

Implementación de un modelo de entorno personal de aprendizaje en el Grado en Educación (Infantil y Primaria. Estudio de caso

Directory of Open Access Journals (Sweden)

Beatriz Tarancón Álvaro

2017-07-01

Full Text Available El advenimiento de las nuevas tecnologías ha supuesto toda una revolución en el ámbito educativo. Tanto docentes como discentes tienen que adaptarse a una nueva realidad que trae consigo una enorme cantidad de retos. En este artículo se pretende mostrar, desde una perspectiva general, un estudio de caso en el que se propone a los alumnos la creación de su propio Entorno Personal de Aprendizaje (PLE como apoyo a la docencia presencial y como herramienta de autogestión de las horas de trabajo autónomo propuestas en el nuevo Plan Bolonia. Se ofrece a los alumnos una plantilla a partir de la cual ellos pueden personalizar su PLE de acuerdo con sus preferencias e intereses. Se lleva a cabo asimismo un seguimiento del proceso a través de encuestas facilitadas a los alumnos (antes, durante y después de la entrega de la plantilla y finalmente, una vez analizados los datos, se extraen una serie de conclusiones al respecto.

Research and performance evaluation on an HA integrated acid system for sandstone acidizing

Directory of Open Access Journals (Sweden)

Liqiang Zhao

2018-03-01

Full Text Available When the conventional sandstone acidizing technologies are adopted, many slugs are needed in the injection of prepad fluid, treatment fluid and postpad fluid, and consequently the production and operation suffers inconveniences and difficulties. In view of this, a kind of HA integrated acid system which is mainly composed of organic polybasic acids (HA+HCl + HF and an efficient organic solvent was developed in this paper based on the idea of integrated acid replacing ''multiple steps'' and high efficiency and intensification. Via this HA integrated acid system, the complicated blockage in sandstone reservoirs can be removed effectively. Then, experiments were carried out on this system to evaluate its performance in terms of its retardance, organic blockage dissolution, chelating and precipitation inhibition. It is indicated that this new system can not only realize the acidizing of conventional integrated acid, but also present a good retarding performance by controlling H+ multi-stage ionization step by step and by forming silica acid-aluminum phosphonate film on the surface of clay minerals; that via this new HA integrated acid system, the organic blockage can be removed efficiently; and that it is wider in pH solution range than conventional APCs (aminopolycarboxyliates chelants, stronger in chelating capacity of Ca2+, Mg2+ and Fe3+ than conventional chelants (e.g. EDTA, NTA and DTPA, and better in precipitation inhibition on metal fluoride, fluosilicic acid alkali metal, fluoaluminic acid alkali metal and hydroxide than multi-hydrogen acid, fluoboric acid and mud acid systems. These research results provide a technical support for the plugging removal in high-temperature deep oil and gas reservoirs. Keywords: Organic polybasic acid, Integrated acid, Retardance, Chelating, Precipitation, Acidizing, Sandstone, Reservoir

Characterization of electrolytic HA/ZrO{sub 2} double layers coatings on Ti-6Al-4V implant alloy

Energy Technology Data Exchange (ETDEWEB)

Yen, S.K. [Department of Materials Engineering, National Chung Hsing University, Taichung 40227, Taiwan (China)]. E-mail: skyen@dragon.nchu.edu.tw; Chiou, S.H. [Graduate Institute of Veterinary Microbiology, National Chung Hsing University, Taichung 40227, Taiwan (China); Wu, S.J. [Department of Materials Engineering, National Chung Hsing University, Taichung 40227, Taiwan (China); Chang, C.C. [Department of Materials Engineering, National Chung Hsing University, Taichung 40227, Taiwan (China); Lin, S.P. [Department of Materials Engineering, National Chung Hsing University, Taichung 40227, Taiwan (China); Lin, C.M. [Department of Materials Engineering, National Chung Hsing University, Taichung 40227, Taiwan (China)

2006-01-15

Hydroxyapatite (HA) coating was proved having bioactive property and hence improving the bonding strength on bone tissue without inducing the growth of fiber tissue. However, the weak adhesion between HA and metal implants is still the major problem. In this study, a novel method of electrolytic HA/ZrO{sub 2} double layers coating was successfully conducted on F-136 Ti-6Al-4V implant alloy in ZrO{sub 2}(NO{sub 3}){sub 2} aqueous solution and subsequently in the mixed solution of Ca(NO{sub 3}){sub 2} and NH{sub 4}H{sub 2}PO{sub 4}. After annealing at 400 deg. C, 500 deg. C and 600 deg. C for 4 h in air, the coated specimens were evaluated by X-ray diffraction analyses, surface morphology observations, scratch tests, dynamic polarization tests, immersion tests and cell culture assays. In addition to corrosion resistance, the adhesion strength of electrolytic deposited HA on Ti alloy was dramatically improved from the critical scratch load 2 N to 32 N by adding the intermediate electrolytic deposition of ZrO{sub 2}, which showed the strong bonding effects between Ti alloy substrate and HA coating. Based on the cell morphology and cell proliferation data, HA/ZrO{sub 2} double layers coating revealed the better substrate for the adhesion and proliferation of osteoblasts than the others. It was also found that the crystallization of HA had positive effect on the proliferation of osteoblasts.

Clinical significance of changes of serum TBA, CG, HA levels in neonate with parenteral nutrition

International Nuclear Information System (INIS)

Huang Weiliang; Zhou Jiongying; Zhang Xiaoyi; Lv Weihua; Ma Yunbao; He Qizhi

2010-01-01

Objective: To study the clinical significance of changes of serum levels of TBA, CG, HA in neonate with parenteral nutrition. Methods: Serum total bile acid (TBA, with biochemistry) and CG, HA (with RIA) contents were measured in 52 neonates (full-term 32, preterm 20) with parenteral nutrition and 28 neonates (full-term 16, preterm 12) without parenteral nutrition (as controls). Results: Before parenteral nutrition,the serum TBA, CG and HA levels in full-term neonates were not significantly different from those in the controls (P>0.05). After parenteral nutrition,serum levels were significantly higher than those before parenteral nutrition (P<0.01). The levels in pre-term neonates were significantly higher after parenteral nutrition than those in full-term neonates (P<0.05). Conclusion: Long term parenteral nutrition might be harmful to hepatic and gall bladder function in neonates especially in premature ones. (authors)

El nombramiento de regidores en Madrid (1700-1759 : procedimiento y documentación

Directory of Open Access Journals (Sweden)

Manuel Salamanca López

2004-01-01

Full Text Available La implantación en Madrid del estatuto de nobleza (1603, y su correspondiente ratificación de 1638, supuso que, progresivamente y llegado el siglo xviii, el Concejo estuviese controlado por una oligarquía de carácter nobiliario. Además, se establecieron una serie de mecanismos encaminados a regular el acceso a las regidurías, conforme a un conjunto de requisitos que todo pretendiente debía reunir Para ello, el aspirante tenía que someterse a una serie de pruebas, que se comenzaron a utilizar en Madrid hacia 1608, con el único fin de esclarecer su procedencia social. Todas estas actuaciones cristalizarían en un procedimiento tipo, que ha llegado, más o menos completo, hasta nuestros días en forma de expediente.The installation in Madrid of the statute of nobility (1603, and their corresponding ratification of 1638, supposed that, progressively and arrived the XVIII century, the Town council is controlled by an oligarchy of nobiliary character. Also, a series of mechanisms guided to regulate the access to the regidurías settied down, according to a group of requirements that all pretender should gather. For it, the applicant had to undergo a series of tests that you began to use in Madrid toward 1608, with the only end of clarifying her social origin. All these performances would crystallize more or less in a procedure type that has arrived, complete, unta our days in file form.

v-Ha-ras oncogene insertion: A model for tumor progression of human small cell lung cancer

International Nuclear Information System (INIS)

Mabry, M.; Nakagawa, Toshitaro; Nelkin, B.D.; McDowell, E.; Gesell, M.; Eggleston, J.C.; Casero, R.A. Jr.; Baylin, S.B.

1988-01-01

Small cell lung cancer (SCLC) manifests a range of phenotypes in culture that may be important in understanding its relationship to non-SCLCs and to tumor progression events in patients. Most SCLC-derived cell lines, termed classic SCLC lines, have properties similar to SCLC tumors in patients. To delineate further the relationships between these phenotypes and the molecular events involved, the authors inserted the v-Ha-ras gene in SCLC cell lines with (biochemical variant) and without (classic) an amplified c-myc gene. These two SCLC subtypes had markedly different phenotypic responses to similar levels of expression of v-Ha-ras RNA. No biochemical or morphologic changes were observed in classic SCLC cells. In contrast, in biochemical variant SCLC cells, v-Ha-ras expression induced features typical of large cell undifferentiated lung carcinoma. Expression of v-Ha-ras in biochemical variant SCLC cells directly demonstrates that important transitions can occur between phenotypes of human lung cancer cells and that these may play a critical role in tumor progression events in patients. The finding provide a model system to study molecular events involved in tumor progression steps within a series of related tumor types

Abnormal MRI in a patient with 'headache with neurological deficits and CSF lymphocytosis (HaNDL)'.

Science.gov (United States)

Yilmaz, A; Kaleagasi, H; Dogu, O; Kara, E; Ozge, A

2010-05-01

A 27-year-old woman was admitted to the Emergency Department with right upper-extremity numbness and mild weakness followed by a bifrontal throbbing headache for 30 min, which was similar to a headache lasting for 12 h that had occurred 3 days ago. Laboratory tests were unremarkable except for cerebrospinal fluid (CSF) lymphocytic pleocytosis. On the following day, a headache episode with left hemiparesis and hemihypoaesthesia, left hemifield visio-spatial inattention, anosagnosia and confusion recurred. The headache was diagnosed as headache and neurological deficits with cerebrospinal fluid lymphocytosis (HaNDL) syndrome according to the criteria of the second edition of the International Classification of Headache Disorders. Simultaneously performed magnetic resonance imaging (MRI) revealed swelling of the grey matter, CSF enhancement in the sulci of the right temporal and occipital regions and hypoperfusion of the same brain regions. During the following 10 days two more similar episodes recurred and during the ensuing 12 months the patient remained headache free. Neuroimaging findings of the HaNDL syndrome are always thought as virtually normal. MRI abnormalities in our patient have not been reported in HaNDL syndrome previously, although they have been reported in hemiplegic migraine patients before. The findings in our case suggest that hemiplegic migraine and HaNDL syndrome may share a common pathophysiological pathway resulting in similar imaging findings and neurological symptoms.

Para una teoría social del acontecimiento

Directory of Open Access Journals (Sweden)

Jaime De la Calle Valverde

2010-07-01

Full Text Available La reflexión en torno al acontecimiento ha tenido cierta tradición en la teoría social francesa del siglo XX. Sin embargo, esa presencia histórica ha sido irregular: junto a etapas de completa invisibilidad han coexistido otras en las que ha tenido algo más de protagonismo. En las décadas de los 70 y de los 80 se reivindica el acontecimiento como respuesta al estructuralismo que acapara la investigación  de las décadas centrales del siglo XX. Se trata de una reivindicación más teórica que empírica, en busca de una salida a los sistemas sociales cerrados descritos por el estructuralismo.Este artículo se propone recuperar ese aporte teórico. Sin embargo, las sociedades contemporáneas parecen ser diferentes de las sociedades firmemente estructuradas que nos fueron descritas en el siglo XX. Nos preguntamos si el acontecimiento es el mismo. Es decir, si el acontecimiento es una vía de cambio en sociedades que viven en un cambio permanente. Se probará, pues, la validez actual de aquellas reflexiones sobre el acontecimiento y, llegado el caso, se actualizará dicha teoría de acuerdo a una nueva realidad contemporánea. 

Zonation of heme synthesis enzymes in mouse liver and their regulation by β-catenin and Ha-ras.

Science.gov (United States)

Braeuning, Albert; Schwarz, Michael

2010-11-01

Cytochrome P450 (CYP) hemoproteins play an important role in hepatic biotransformation. Recently, β-catenin and Ha-ras signaling have been identified as players controlling transcription of various CYP genes in mouse liver. The aim of the present study was to analyze the role of β-catenin and Ha-ras in the regulation of heme synthesis. Heme synthesis-related gene expression was analyzed in normal liver, in transgenic mice expressing activated β-catenin or Ha-ras, and in hepatomas. Regulation of the aminolevulinate dehydratase promoter was studied in vitro. Elevated expression of mRNAs and proteins involved in heme biosynthesis was linked to β-catenin activation in perivenous hepatocytes, in transgenic hepatocytes, and in hepatocellular tumors. Stimulation of the aminolevulinate dehydratase promoter by β-catenin was independent of the β-catenin/T-cell-specific transcription factor dimer. By contrast, activation of Ha-ras repressed heme synthesis-related gene expression. The present data suggest that β-catenin enhances the expression of both CYPs and heme synthesis-related genes, thus coordinating the availability of CYP apoprotein and its prosthetic group heme. The reciprocal regulation of heme synthesis by β-catenin and Ha-ras-dependent signaling supports our previous hypothesis that antagonistic action of these pathways plays a major role in the control of zonal gene expression in healthy mouse liver and aberrant expression patterns in hepatocellular tumors.

Processing of influenza HA protein in MDCK cells: components with different mobilities in polyacrylamide gel electrophoresis and their precursor-product relationships

International Nuclear Information System (INIS)

Sklyanskaya, E.I.; Rudneva, I.A.; Vovk, T.S.; Kaverin, N.V.

1980-01-01

In influenza virus-infected MDCK cells labelled with 14 C-chlorella hydrolysate or 35 S-methionine a virus-specific protein component is revealed migrating slightly faster than HA protein in polyacrylamide gel electrophoresis. Under chase conditions the component disappears either completely or partially, with a concomitant intensification of the HA band. The rate and extent of this transition are strain-dependent. Both the HA band and the faster moving component are not revealed if the cells are labelled in the presence of 20 mM of D-glucosamine. In primary cell cultures of chick embryos a single HA band with a mobility similar to that of the faster moving component in MDCK cells has been observed. It is suggested that the transition of the label from the faster moving component to the HA band reflects the final step of HA processing specific for MDCK cells. (author)

La gestion des ressources naturelles dans la commune de Hong Ha ...

International Development Research Centre (IDRC) Digital Library (Canada)

approvisionnement utilisée par l'armée nord-vietnamienne dans les années 1960 et 1970, la commune de Hong Ha — qui .... J'ai remboursé le prêt et utilisé les profits pour acheter de la nourriture et envoyer mes enfants à l'école. ».

Videojuegos como herramienta en Educación Primaria: caso de estudio con eAdventure

Directory of Open Access Journals (Sweden)

Solano Nogales, Luis

2016-12-01

Full Text Available Los videojuegos son herramientas interactivas y sociales de aprendizaje de la Web 2.0, con las que los usuarios pueden jugar, participar, comunicarse y aprender, creando y sacando partido de los contenidos. No obstante el profesorado necesita formación para exprimir de forma eficaz estas herramientas. Con el ánimo de comprobar la eficacia del videojuego como herramienta de enseñanza en el aula, en este artículo se presenta un estudio de caso de la adopción de los videojuegos en Educación Primaria, donde la herramienta eAdventure se ha utilizado para impartir una Unidad Didáctica de la asignatura Ciencias de la Naturaleza a alumnos de siete años. La conclusión a la que se ha llegado tras su realización es que los videojuegos pueden ser utilizados como herramientas educativas y las principales fortalezas de su uso en clase son: (i la reducción en la temporalización a la hora de impartir un contenido y (ii el incremento en la atención de los alumnos.

La estructura de capital en las medianas empresas del departamento de Boyacá-Colombia

Directory of Open Access Journals (Sweden)

Andrés Fernando Mejía Amaya

2015-06-01

Full Text Available El estudio de la estructura de capital es uno de los tópicos financieros de mayor impacto, puesto que entre las teorías que intentan explicarla todavía no se ha llegado a un consenso sobre las determinantes y el comportamiento de las decisiones de financiamiento en las empresas. La investigación sobre el comportamiento de la estructura de capital en las empresas de tamaño medio y pequeño es un asunto aún más complejo, pues los modelos tradicionales son aplicables principalmente a empresas que se cotizan en los mercados de capitales o son de interés público. Para este trabajo se seleccionaron las medianas empresas en el departamento de Boyacá en Colombia como población por estudiar, aplicando un modelo que se ha utilizado en casos similares en otros países; se demostró empíricamente la preferencia por la financiación con recursos propios antes que con deuda, la cual, a su vez, se prefirió por las empresas con mayor volatilidad en su rentabilidad y por las de mayor crecimiento.

Rheological and structural characterization of HA/PVA-SbQ composites film-forming solutions and resulting films as affected by UV irradiation time.

Science.gov (United States)

Bai, Huiyu; Sun, Yunlong; Xu, Jing; Dong, Weifu; Liu, Xiaoya

2015-01-22

Hyaluronan (HA)/poly (vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ) composites film-forming solutions were prepared by a negatively charged HA and an oppositely charged PVA-SbQ. The rheological properties and structural characterization of HA/PVA-SbQ composites in aqueous solution were investigated. Zeta potential measurements and TEM were utilized to explore the formation of HA/PVA-SbQ complex micelles in aqueous solution. UV spectra and DLS experiments confirmed that the micelles are photo-crosslinkable. HA/PVA-SbQ composites films were prepared by a casting method. The microstructure and properties of the film were analyzed by SEM, optical transmittance, DSC, XRD and tensile testing. The crosslinked HA/PVA-SbQ composites films exhibited higher UV light shielding and visible light transparency and better mechanical and water vapor barrier properties as well as thermal stability than the uncrosslinked HA/PVA-SbQ composites films, indicating the formation of three-dimensional network structure. This work provided a good way for increasing the mechanical, thermal, water vapor barrier, and optical properties of HA materials for the packaging material. Copyright © 2014 Elsevier Ltd. All rights reserved.

The novel kinetics expression of Cadmium (II) removal using green adsorbent horse dung humic acid (Hd-Ha)

Science.gov (United States)

Basuki, Rahmat; Santosa, Sri Juari; Rusdiarso, Bambang

2017-03-01

Humic acid from dry horse dung powder has been prepared and this horse dung humic acid (HD-HA) was then applied as a sorbent to adsorb Cadmium(II) from a solution. Characterization of HD-HA was conducted by detection of its functional group, UV-Vis spectra, ash level, and total acidity. Result of the work showed that HD-HA had similar character compared with peat soil humic acid (PS-HA) and previous researchers. The adsorption study of this work was investigated by batch experiment in pH 5. The thermodynamics parameters in this work were determined by the Langmuir isotherm model for monolayer sorption and Freundlich isotherm model multilayer sorption. Monolayer sorption capacity (b) for HD-HA was 1.329 × 10-3 mol g-1, equilibrium constant (K) was 5.651 (mol/L)-1, and multilayer sorption capacity was 2.646 × 10-2 mol g-1. The kinetics parameters investigated in this work were determined by the novel kinetics expression resulted from the mathematical derivation the availability of binding sites of sorbent. Adsorption rate constant (ka) from this novel expression was 43.178 min-1 (mol/L)-1 and desorption rate constant (kd) was 1.250 × 10-2 min-1. Application of the kinetics model on sorption Cd(II) onto HD-HA showed the nearly all of models gave a good linearity. However, only this proposed kinetics expression has good relation with Langmuir model. The novel kinetics expression proposed in this paper seems to be more realistic and reasonable and close to the experimental real condition because the value of ka/kd (3452 (mol/L)-1) was fairly close with K from Langmuir isotherm model (5651 (mol/L)-1). Comparison of this novel kinetics expression with well-known Lagergren pseudo-first order kinetics and Ho pseudo-second order kinetics was also critically discussed in this paper.

Effect of gamma radiation and accelerated aging on the mechanical and thermal behavior of HDPE/HA nano-composites for bone tissue regeneration.

Science.gov (United States)

Alothman, Othman Y; Almajhdi, Fahad N; Fouad, H

2013-09-24

The replacement of hard tissues demands biocompatible and sometimes bioactive materials with properties similar to those of bone. Nano-composites made of biocompatible polymers and bioactive inorganic nano particles such as HDPE/HA have attracted attention as permanent bone substitutes due to their excellent mechanical properties and biocompatibility. The HDPE/HA nano-composite is prepared using melt blending at different HA loading ratios. For evaluation of the degradation by radiation, gamma rays of 35 kGy, and 70 kGy were used to irradiate the samples at room temperature in vacuum. The effects of accelerated ageing after gamma irradiation on morphological, mechanical and thermal properties of HDPE/HA nano-composites were measured. In Vitro test results showed that the HDPE and all HDPE/HA nano-composites do not exhibit any cytotoxicity to WISH cell line. The results also indicated that the tensile properties of HDPE/HA nano-composite increased with increasing the HA content except fracture strain decreased. The dynamic mechanical analysis (DMA) results showed that the storage and loss moduli increased with increasing the HA ratio and the testing frequency. Finally, it is remarked that all properties of HDPE/HA is dependent on the irradiation dose and accelerated aging. Based on the experimental results, it is found that the addition of 10%, 20% and 30% HA increases the HDPE stiffness by 23%, 44 and 59% respectively. At the same time, the G' increased from 2.25E11 MPa for neat HDPE to 4.7E11 MPa when 30% HA was added to the polymer matrix. Also, significant improvements in these properties have been observed due to irradiation. Finally, the overall properties of HDPE and its nano-composite properties significantly decreased due to aging and should be taken into consideration in the design of bone substitutes. It is attributed that the developed HDPE/HA nano-composites could be a good alternative material for bone tissue regeneration due to their acceptable

Programming Algorithms of load balancing with HA-Proxy in HTTP services

Directory of Open Access Journals (Sweden)

José Teodoro Mejía Viteri

2018-02-01

Full Text Available The access to the public and private services through the web gains daily protagonism, and sometimes they must support amounts of requests that a team can not process, so there are solutions that use algorithms that allow to distribute the load of requests of a web application in several equipment; the objective of this work is to perform an analysis of load balancing scheduling algorithms through the HA-Proxy tool, and deliver an instrument that identifies the load distribution algorithm to be used and the technological infrastructure, to largely cover implementation. The information used for this work is based on a bibliographic analysis, eld study and implementation of the different load balancing algorithms in equipment, where the distribution and its performance will be analyzed. The incorporation of this technology to the management of services on the web, improves availability, helps business continuity and through the different forms of distribution of the requests of the algorithms that can be implemented in HA-Proxy to provide those responsible for information technology systems with a view of their advantages and disadvantages.

IDENTIDAD y RECONFIGURACIóN TIEMPO–ESPACIO DE LOS DESPLAZADOS HACIA MEDELLíN: LECTURA EN LAS PRáCTICAS DE OCIO

Directory of Open Access Journals (Sweden)

Rubiela Arboleda Gómez

2015-11-01

Full Text Available Este artículo es producto de la investigación "El ocio en la reconfiguración identitaria de los desplazados hacia la ciudad de Medellín" (2011-2013. La disertación pretende dar cuenta del tiempo en relación con el espacio, leído desde las prácticas de ocio, y su significado en la emergencia de identidades, consecuencia del destierro. Metodológicamente, se sitúa en el marco de la etnografía reflexiva implementada con entrevistas y diarios de campo. Por este medio, se ha llegado a comprender que la modificación en la percepción del tiempo en el tránsito rural–urbano atraviesa las prácticas de ocio y se traduce ontológicamente en identidades emergentes.

El error en el delito imprudente

Directory of Open Access Journals (Sweden)

Miguel Angel Muñoz García

2011-12-01

Full Text Available La teoría del error en los delitos culposos constituye un tema álgido de tratar, y controversial en la dogmática penal: existen en realidad muy escasas referencias, y no se ha llegado a un consenso razonable. Partiendo del análisis de la estructura dogmática del delito imprudente, en donde se destaca el deber objetivo de cuidado como elemento del tipo sobre el que recae el error, y de las diferentes posiciones doctrinales que defienden la aplicabilidad del error de tipo y del error de prohibición, se plantea la viabilidad de este último, con fundamento en razones dogmáticas y de política criminal, siendo la infracción del deber objetivo de cuidado en tanto consecuencia del error, un tema por analizar en sede de culpabilidad.

Cooperación para el desarrollo en Brasil : evaluación de proyectos de formación

Directory of Open Access Journals (Sweden)

María Angeles Goicoechea Gaona

2013-05-01

Full Text Available El estudio que recoge este artículo, se refiere a la evaluación de los proyectos realizados por la Asociación Lar Santa Maria y Obra Social Santa Rita de Casia en dos enclaves desfavorecidos del Estado de Sao Paulo (Brasil. Dichas asociaciones, financiadas en parte por entidades riojanas, forman a jóvenes y adultos para favorecer su inserción y mejora en el mundo laboral. Después de casi diez años de trabajo con la primera de estas asociaciones, se ha realizado una recopilación de datos a través de la observación, realización de encuestas y entrevistas, todo ello sobre el terreno. Presentamos los datos obtenidos, el análisis de los mismos y las conclusiones a las que hemos llegado.

Visualización de arquitectura y género. ¿Un terreno igualitario?

Directory of Open Access Journals (Sweden)

María Novas Ferradás

2017-07-01

Full Text Available ¿Es la visualización de arquitectura un terreno igualitario? ¿Existen sesgos de género en esta disciplina? ¿En qué consiste incorporar la perspectiva de género en la visualización de la arquitectura? La industria CGI, en su vertiente arquitectónica, denominada visualización de arquitectura, rendering o archViz, es considerada una forma eficaz de comunicar arquitectura que ha llegado a ser definida como un auténtico “porno para arquitectos”. El rendering busca ensalzar nuestras fantasías arquitectónicas, invitándonos a consumir, a través de un trabajo estético muy cuidado, una arquitectura feliz. Es la representación de un ideal en cuya evolución todavía resta pendiente una lectura de género.

Single-dose monomeric HA subunit vaccine generates full protection from influenza challenge

CSIR Research Space (South Africa)

Mallajosyula, JK

2014-03-01

Full Text Available 50% survival, or 100% survival with adjuvant, compared with 10% survival after vaccination with a commercially available H 1 N 1 vaccine. TMV-HA is an effective dose-sparing influenza vaccine, using a single-step process to rapidly generate large...

Groteskno Haškových cestovních povídek

Czech Academy of Sciences Publication Activity Database

Hrbata, Zdeněk

2014-01-01

Roč. 61, č. 3 (2014), s. 181-198 ISSN 0037-6973 R&D Projects: GA ČR GA13-29985S Institutional support: RVO:68378068 Keywords : travel ogues * grotesqueness * parody * Hašek, Jaroslav Subject RIV: AJ - Letters, Mass-media, Audiovision

The Study on Biocompatibility of Porous nHA/PLGA Composite Scaffolds for Tissue Engineering with Rabbit Chondrocytes In Vitro

Directory of Open Access Journals (Sweden)

Lei Chen

2013-01-01

Full Text Available Objective. To examine the biocompatibility of a novel nanohydroxyapatite/poly[lactic-co-glycolic acid] (nHA/PLGA composite and evaluate its feasibility as a scaffold for cartilage tissue engineering. Methods. Chondrocytes of fetal rabbit were cultured with nHA/PLGA scaffold in vitro and the cell viability was assessed by MTT assay first. Cells adhering to nHA/PLGA scaffold were then observed by inverted microscope and scanning electron microscope (SEM. The cell cycle profile was analyzed by flow cytometry. Results. The viability of the chondrocytes on the scaffold was not affected by nHA/PLGA comparing with the control group as it was shown by MTT assay. Cells on the surface and in the pores of the scaffold increased in a time-dependent manner. Results obtained from flow cytometry showed that there was no significant difference in cell cycle profiles between the coculture group and control (P>0.05. Conclusion. The porous nHA/PLGA composite scaffold is a biocompatible and good kind of scaffold for cartilage tissue engineering.

Comparison between PCL/hydroxyapatite (HA) and PCL/halloysite nanotube (HNT) composite scaffolds prepared by co-extrusion and gas foaming.

Science.gov (United States)

Jing, Xin; Mi, Hao-Yang; Turng, Lih-Sheng

2017-03-01

In this work, three-dimensional poly(caprolactone) (PCL) tissue engineering scaffolds were prepared by co-extrusion and gas foaming. Biocompatible hydroxyapatite (HA) and halloysite nanotubes (HNT) were added to the polymer matrix to enhance the mechanical properties and bioactivity of the composite scaffolds. The effects of HA and HNT on the rheological behavior, microstructure, and mechanical properties of the composite scaffolds were systematically compared. It was found that the HNT improved viscosity more significantly than HA, and reduced the pore size of scaffolds, while the mechanical performance of PCL/HNT scaffolds was higher than PCL/HA scaffolds with the same filler content. Human mesenchymal stem cells (hMSCs) were used as the cell model to compare the biological properties of two composite scaffolds. The results demonstrated that cells could survive on all scaffolds, and showed a more flourishing living state on the composite scaffolds. The cell differentiation for 5% HA and 1% HNT scaffolds were significantly higher than other scaffolds, while the differentiation of 5% HNT scaffolds was lower than that of 1% HNT scaffolds mainly because of the reduced pore size and pore interconnectivity. Therefore, this study suggested that, with proper filler content and control of microstructure through processing, HNT could be a suitable substitute for HA for bone tissue engineering to reduce the cost and improve mechanical performance. Copyright © 2016. Published by Elsevier B.V.

Metallpitsid Tallinna Püha Johannese hospidali (Jaani seegi) kalmistu haualeidudes / Sari Marjut Rainne

Index Scriptorium Estoniae

Rainne, Sari Marjut

2010-01-01

Tallinna Püha Johannese hospidali (Jaani seegi) kalmistualal toimunud arheoloogilistelt kaevamistelt leitud metallpitsidest. Ülevaade põhiliselt 18. sajandist pärit peakatete külge kinnitatud pitside materjaliuuringu tulemustest ja konserveerimisest

Crystal Structure of the Receptor-Binding Domain of Botulinum Neurotoxin Type HA, Also Known as Type FA or H.

Science.gov (United States)

Yao, Guorui; Lam, Kwok-Ho; Perry, Kay; Weisemann, Jasmin; Rummel, Andreas; Jin, Rongsheng

2017-03-08

Botulinum neurotoxins (BoNTs), which have been exploited as cosmetics and muscle-disorder treatment medicines for decades, are well known for their extreme neurotoxicity to humans. They pose a potential bioterrorism threat because they cause botulism, a flaccid muscular paralysis-associated disease that requires immediate antitoxin treatment and intensive care over a long period of time. In addition to the existing seven established BoNT serotypes (BoNT/A-G), a new mosaic toxin type termed BoNT/HA (aka type FA or H) was reported recently. Sequence analyses indicate that the receptor-binding domain (H C ) of BoNT/HA is ~84% identical to that of BoNT/A1. However, BoNT/HA responds differently to some potent BoNT/A-neutralizing antibodies (e.g., CR2) that target the H C . Therefore, it raises a serious concern as to whether BoNT/HA poses a new threat to our biosecurity. In this study, we report the first high-resolution crystal structure of BoNT/HA-H C at 1.8 Å. Sequence and structure analyses reveal that BoNT/HA and BoNT/A1 are different regarding their binding to cell-surface receptors including both polysialoganglioside (PSG) and synaptic vesicle glycoprotein 2 (SV2). Furthermore, the new structure also provides explanations for the ~540-fold decreased affinity of antibody CR2 towards BoNT/HA compared to BoNT/A1. Taken together, these new findings advance our understanding of the structure and function of this newly identified toxin at the molecular level, and pave the way for the future development of more effective countermeasures.

Crystal Structure of the Receptor-Binding Domain of Botulinum Neurotoxin Type HA, Also Known as Type FA or H

Directory of Open Access Journals (Sweden)

Guorui Yao

2017-03-01

Full Text Available Botulinum neurotoxins (BoNTs, which have been exploited as cosmetics and muscle-disorder treatment medicines for decades, are well known for their extreme neurotoxicity to humans. They pose a potential bioterrorism threat because they cause botulism, a flaccid muscular paralysis-associated disease that requires immediate antitoxin treatment and intensive care over a long period of time. In addition to the existing seven established BoNT serotypes (BoNT/A–G, a new mosaic toxin type termed BoNT/HA (aka type FA or H was reported recently. Sequence analyses indicate that the receptor-binding domain (HC of BoNT/HA is ~84% identical to that of BoNT/A1. However, BoNT/HA responds differently to some potent BoNT/A-neutralizing antibodies (e.g., CR2 that target the HC. Therefore, it raises a serious concern as to whether BoNT/HA poses a new threat to our biosecurity. In this study, we report the first high-resolution crystal structure of BoNT/HA-HC at 1.8 Å. Sequence and structure analyses reveal that BoNT/HA and BoNT/A1 are different regarding their binding to cell-surface receptors including both polysialoganglioside (PSG and synaptic vesicle glycoprotein 2 (SV2. Furthermore, the new structure also provides explanations for the ~540-fold decreased affinity of antibody CR2 towards BoNT/HA compared to BoNT/A1. Taken together, these new findings advance our understanding of the structure and function of this newly identified toxin at the molecular level, and pave the way for the future development of more effective countermeasures.

Crystal Structure of the Receptor-Binding Domain of Botulinum Neurotoxin Type HA, Also Known as Type FA or H

Energy Technology Data Exchange (ETDEWEB)

Yao, Guorui; Lam, Kwok-ho; Perry, Kay; Weisemann, Jasmin; Rummel, Andreas; Jin, Rongsheng (Cornell); (Dusseldorf); (UCI)

2017-03-01

Botulinum neurotoxins (BoNTs), which have been exploited as cosmetics and muscle-disorder treatment medicines for decades, are well known for their extreme neurotoxicity to humans. They pose a potential bioterrorism threat because they cause botulism, a flaccid muscular paralysis-associated disease that requires immediate antitoxin treatment and intensive care over a long period of time. In addition to the existing seven established BoNT serotypes (BoNT/A–G), a new mosaic toxin type termed BoNT/HA (aka type FA or H) was reported recently. Sequence analyses indicate that the receptor-binding domain (HC) of BoNT/HA is ~84% identical to that of BoNT/A1. However, BoNT/HA responds differently to some potent BoNT/A-neutralizing antibodies (e.g., CR2) that target the HC. Therefore, it raises a serious concern as to whether BoNT/HA poses a new threat to our biosecurity. In this study, we report the first high-resolution crystal structure of BoNT/HA-HC at 1.8 Å. Sequence and structure analyses reveal that BoNT/HA and BoNT/A1 are different regarding their binding to cell-surface receptors including both polysialoganglioside (PSG) and synaptic vesicle glycoprotein 2 (SV2). Furthermore, the new structure also provides explanations for the ~540-fold decreased affinity of antibody CR2 towards BoNT/HA compared to BoNT/A1. Taken together, these new findings advance our understanding of the structure and function of this newly identified toxin at the molecular level, and pave the way for the future development of more effective countermeasures

The sunflower transcription factor HaHB11 confers tolerance to water deficit and salinity to transgenic Arabidopsis and alfalfa plants.

Science.gov (United States)

Cabello, Julieta V; Giacomelli, Jorge I; Gómez, María C; Chan, Raquel L

2017-09-10

Homeodomain-leucine zipper (HD-Zip) transcription factors are unique to the plant kingdom; members of subfamily I are known to be involved in abiotic stress responses. HaHB11 belongs to this subfamily and it was previously shown that it is able to confer improved yield and tolerance to flooding via a quiescent strategy. Here we show that HaHB11 expression is induced by ABA, NaCl and water deficit in sunflower seedlings and leaves. Arabidopsis transgenic plants expressing HaHB11, controlled either by its own promoter or by the constitutive 35S CaMV, presented rolled leaves and longer roots than WT when grown under standard conditions. In addition, these plants showed wider stems and more vascular bundles. To deal with drought, HaHB11 transgenic plants closed their stomata faster and lost less water than controls, triggering an enhanced tolerance to such stress condition and also to salinity stress. Concomitantly, ABA-synthesis and sensing related genes were differentially regulated in HaHB11 transgenic plants. Either under long-term salinity stress or mild drought stress, HaHB11 transgenic plants did not exhibit yield penalties. Moreover, alfalfa transgenic plants were generated which also showed enhanced drought tolerance. Altogether, the results indicated that HaHB11 was able to confer drought and salinity tolerance via a complex mechanism which involves morphological, physiological and molecular changes. Copyright © 2016 Elsevier B.V. All rights reserved.

Synthesis and Characterization of Hydroxyapatite-Collagen-Chitosan (HA/Col/Chi) Composite Coated on Ti6Al4V

Science.gov (United States)

Charlena; Bikharudin, Ahmad; Wahyudi, Setyanto Tri

2018-01-01

HA-collagen-chitosan (HA/col/chi) composite is developed to increase bioactivity adhesiveness between the metal and the material composite and to improve corrosion resistance. The Ti6Al4V alloy was coated by soaking in HA/col/chi composite at room temperature and then allowed to stand for 5, 6, and 7 days. Diffraction pattern analysis of the coated Ti6Al4V alloy showed that the dominant phase were HA and Ti6Al4V alloy. Corrosion resistance test in media by using 0.9% NaCl showed the corrosion rate at the level of 0.3567 mpy, which was better than that of the uncoated Ti6Al4V alloy (0.4152 mpy). In vitro cytocompatibility assay on endothelial cell of calf pulmonary artery endothelium (CPAE) (ATCC-CCL 209) showed there was no toxicity in the cell culture with the percent inhibition of 33.33% after 72 hours of incubation.

Wear Behavior of Plasma Spray Deposited and Post Heat-Treated Hydroxyapatite (HA)-Based Composite Coating on Titanium Alloy (Ti-6Al-4V) Substrate

Science.gov (United States)

Kumari, Renu; Majumdar, Jyotsna Dutta

2018-04-01

The present study concerns a detailed evaluation of wear resistance property of plasma spray deposited composite hydroxyapatite (HA)-based (HA-50 wt pct TiO2 and HA-10 wt pct ZrO2) bioactive coatings developed on Ti-6Al-4V substrate and studying the effect of heat treatment on it. Heat treatment of plasma spray deposited samples has been carried out at 650 °C for 2 hours (for HA-50 wt pct TiO2 coating) and at 750 °C for 2 hours (for HA-10 wt pct ZrO2 coating). There is significant deterioration in wear resistance for HA-50 wt pctTiO2 coating and a marginal deterioration in wear resistance for HA-10 wt pct ZrO2 coating in as-sprayed state (as compared to as-received Ti-6Al-4V) which is, however, improved after heat treatment. The coefficient of friction is marginally increased for both HA-50 wt pct TiO2 and HA-10 wt pct ZrO2 coatings in as-sprayed condition as compared to Ti-6Al-4V substrate. However, coefficient of friction is decreased for both HA-50 wt pct TiO2 and HA-10 wt pct ZrO2 coatings after heat-treated condition as compared to Ti-6Al-4V substrate. The maximum improvement in wear resistance property is, however, observed for HA-10 wt pct ZrO2 sample after heat treatment. The mechanism of wear has been investigated.

Recombinant HA1 produced in E. coli forms functional oligomers and generates strain-specific SRID potency antibodies for pandemic influenza vaccines.

Science.gov (United States)

Khurana, Surender; Larkin, Christopher; Verma, Swati; Joshi, Manju B; Fontana, Juan; Steven, Alasdair C; King, Lisa R; Manischewitz, Jody; McCormick, William; Gupta, Rajesh K; Golding, Hana

2011-08-05

Vaccine production and initiation of mass vaccination is a key factor in rapid response to new influenza pandemic. During the 2009-2010 H1N1 pandemic, several bottlenecks were identified, including the delayed availability of vaccine potency reagents. Currently, antisera for the single-radial immunodiffusion (SRID) potency assay are generated in sheep immunized repeatedly with HA released and purified after bromelain-treatment of influenza virus grown in eggs. This approach was a major bottleneck for pandemic H1N1 (H1N1pdm09) potency reagent development in 2009. Alternative approaches are needed to make HA immunogens for generation of SRID reagents in the shortest possible time. In this study, we found that properly folded recombinant HA1 globular domain (rHA1) from several type A viruses including H1N1pdm09 and two H5N1 viruses could be produced efficiently using a bacterial expression system and subsequent purification. The rHA1 proteins were shown to form functional oligomers of trimers, similar to virus derived HA, and elicited high titer of neutralizing antibodies in rabbits and sheep. Importantly, the immune sera formed precipitation rings with reference antigens in the SRID assay in a dose-dependent manner. The HA contents in multiple H1N1 vaccine products from different manufacturers (and in several lots) as determined with the rHA1-generated sheep sera were similar to the values obtained with a traditionally generated sheep serum from NIBSC. We conclude that bacterially expressed recombinant HA1 proteins can be produced rapidly and used to generate SRID potency reagents shortly after new influenza strains with pandemic potential are identified. Published by Elsevier Ltd.

Green Chemistry; Sviluppo sostenibile. L'industria ha bisogno del contributo di tutti

Energy Technology Data Exchange (ETDEWEB)

Ingallina, P. [EniTecnologie SpA, San Donato Milanese, MI (Italy)

2001-02-01

Everyone acknowledges that chemistry is a key science in order to study and solve the problems of the environment: a successful arranging technological progress with environment protection is one of the main challenge of the next millennium. The Green Chemistry (or Sustainable Chemistry) represents the specific contribution that chemists can supply for an environmentally compatible development. [Italian] Fabrizio d'Adda, attualmente Presidente di EniChem e membro di Cefic (European Chemical Industry Council), ha aperto il seminario {sup T}he Greening of Chemistry{sup (}EniTecnologie - 31 Ottobre 2000) con un breve discorso. Ha espresso parole di fiducia riguardo al futuro dell'industria chimica, l'unica a suo giudizio, in grado di avviare un nuovo modello di sviluppo nel rispetto delle implicazioni ambientali, sociali ed economiche.

Mistisisme dalam Perspektif Filsafat Analitik: Antara Wittgenstein dan Mehdi Ha'iri Yazdi

Directory of Open Access Journals (Sweden)

M. Hidayatullah S.

2007-12-01

Full Text Available Language is a principal instrument for philosopher and as a medium for analysis and reflection. Wittgenstein said, that the order analytical philosophy is to clean away the epistemology from the "good" terms but unclear in it interpretation. Every thing that can be thinks must be able spoken. The limits of my language mean the limits of my world. From this perspective mysticism and mystics experience is nin-sense, because nothing factual reference. From this case, the goal of this study is to find out the epistemology in mysticism and mystic experience. In the study we include the knowledge by presence (ilm al-hudhuri Ha 'iri as an approach. Ha 'iri said that there is a other knowledge which that can be explanations in limits of language and picture theory. This is knowledge by peresence, which by Wittgenstein aware as some thing which having difference language games from knowledge by correspondece.

Postoperative intra-abdominal collections using a sodium hyaluronate-carboxymethylcellulose (HA-CMC) barrier at the time of laparotomy for uterine or cervical cancers.

Science.gov (United States)

Leitao, Mario M; Byrum, Graham V; Abu-Rustum, Nadeem R; Brown, Carol L; Chi, Dennis S; Sonoda, Yukio; Levine, Douglas A; Gardner, Ginger J; Barakat, Richard R

2010-11-01

A prior analysis of patients undergoing laparotomy for ovarian malignancies at our institution revealed an increased rate of intra-abdominal collections using HA-CMC film during debulking surgery. The primary objective of the current study was to determine whether the use of HA-CMC is associated with the development of postoperative intra-abdominal collections in patients undergoing laparotomy for uterine or cervical malignancies. We retrospectively identified all laparotomies performed for these malignancies from 3/1/05 to 12/31/07. We identified cases involving the use of HA-CMC via billing records and operative reports. Intra-abdominal collections were defined as localized intraperitoneal fluid accumulations in the absence of re-accumulating ascites. We noted incidences of intra-abdominal collections, as well as other complications. Appropriate statistical tests were applied using SPSS 15.0. We identified 169 laparotomies in which HA-CMC was used and 347 in which HA-CMC was not used. The following were statistically similar in both cohorts: age, body mass index (BMI), primary site, surgery for recurrent disease, prior intraperitoneal surgery, and extent of current surgery. Intra-abdominal collections were seen in 6 (3.6%) of 169 HA-CMC cases compared to 10 (2.9%) of 347 non-HA-CMC cases (p=0.7). The rate of infected collections was similar in both groups (1.2% vs. 1.4%). In the subgroup that underwent tumor debulking, intra-abdominal collections were seen in 3 (11.5%) of 26 HA-CMC cases compared to 2 (5.4%) of 37 non-HA-CMC cases (p=0.6). HA-CMC use does not appear to be associated with postoperative intra-abdominal collections in patients undergoing laparotomy for uterine or cervical cancer. Copyright © 2010 Elsevier Inc. All rights reserved.

Study on the changes of serum HA and CG levels in several diseases besides liver disorders

International Nuclear Information System (INIS)

Lu Zhiping; Ma Yunbao; Zhang Xiaoyi; Lv Weihua

2005-01-01

Objective: To study the changes of serum hyaluronic acid (HA) and cholyglycine (CG) in several diseases besides liver disorders. Methods: Serum HA and CG levels were measured with RIA in 78 patients with chronic liver diseases (with 70 controls), (84 patients with primary hepatic carcinoma ), 70 pediatric patients with various infections diseases (with 40 controls), 50 pediatric patients with recurrent respiratory infection (RRI, with 30 controls) and 428 pregnant women ( with 60 controls). In addition, RBC C 3 b receptor ratio (RBCC 3 bRR) and RBC immune-complex ratio (RBCICR) (both with yeast rosette method) as well as serum IgG,IgM,IgA,C 3 levels (with immunoturbidity test) were examined in the 50 RRI pediatric patients, ALT levels were examined in the 70 pediatric patients with infectious diseases and SF examined in the 78 patients with chronic liver diseases. Results: The serum CG, HA and SF levels in the three groups of patients with chronic liver diseases ( CPH, CAH, Cirrhosis) were significantly higher than those in the controls (all P 0.05). In patients with hepatic carcinoma, the CG and HA levels were positively correlated with the mortality at 4 months (P 0.05). For ALT, levels only increased in patients with hepatitis and typhoid fever with none in bacillary dysentery and mumps. In pediatric patients with RRI, RBCC 3 bRR (%), CG, C 3 , IgG levels were significantly higher than those in controls (P 0.05). Conclusion: Various infectious diseases in pediatric patients could induce mild liver damage, which was closely related to the depressed immune status. High CG and HA levels in liver cancer patients suggested high mortality at 4 months. Women in late pregnancy might harbour disturbances in CG metabolism and mild liver injury though without overt symptoms. (authors)

Conformational heterogeneity of the bacteriopheophytin electron acceptor HA in reaction centers from Rhodopseudomonas viridis revealed by Fourier transform infrared spectroscopy and site-directed mutagenesis.

Science.gov (United States)

Breton, J; Bibikova, M; Oesterhelt, D; Nabedryk, E

1999-08-31

The light-induced Fourier transform infrared (FTIR) difference spectra corresponding to the photoreduction of either the HA bacteriopheophytin electron acceptor (HA-/HA spectrum) or the QA primary quinone (QA-/QA spectrum) in photosynthetic reaction centers (RCs) of Rhodopseudomonas viridis are reported. These spectra have been compared for wild-type (WT) RCs and for two site-directed mutants in which the proposed interactions between the carbonyls on ring V of HA and the RC protein have been altered. In the mutant EQ(L104), the putative hydrogen bond between the protein and the 9-keto C=O of HA should be affected by changing Glu L104 to a Gln. In the mutant WF(M250), the van der Waals interactions between Trp M250 and the 10a-ester C=O of HA should be modified. The characteristic effects of both mutations on the FTIR spectra support the proposed interactions and allow the IR modes of the 9-keto and 10a-ester C=O of HA and HA- to be assigned. Comparison of the HA-/HA and QA-/QA spectra leads us to conclude that the QA-/QA IR signals in the spectral range above 1700 cm-1 are largely dominated by contributions from the electrostatic response of the 10a-ester C=O mode of HA upon QA photoreduction. A heterogeneity in the conformation of the 10a-ester C=O mode of HA in WT RCs, leading to three distinct populations of HA, appears to be related to differences in the hydrogen-bonding interactions between the carbonyls of ring V of HA and the RC protein. The possibility that this structural heterogeneity is related to the observed multiexponential kinetics of electron transfer and the implications for primary processes are discussed. The effect of 1H/2H exchange on the QA-/QA spectra of the WT and mutant RCs shows that neither Glu L104 nor any other exchangeable carboxylic residue changes appreciably its protonation state upon QA reduction.

Study on the clinical value of determination of serum adenosine deaminase (ADA) and HA, PIIIP levels for diagnosis of chronic hepatitis

International Nuclear Information System (INIS)

Ma Yun

2007-01-01

Objective: To explore the clinical value of determination of serum ADA and HA, PIIIP levels for diagnosis of chronic hepatitis. Methods: Serum ADA levels were determined with biochemistry and HA, PIIIP levels were detected with RIA in 36 patients with chronic hepatitis and 35 controls. Results: The levels of serum ADA, HA and PIIIP were significantly higher in the patients than those in controls (P<0.01). Serum ADA, HA, PIIIP levelers were mutually positively correlated (r=0.6117, 0.7134, P<0.01). Conclusion: Detection of serum ADA levels could reflect the liver reserve function and status of fibrosis. (authors)

Correlations between the in vitro and in vivo bioactivity of the Ti/HA composites fabricated by a powder metallurgy method.

Science.gov (United States)

Ning, Congqin; Zhou, Yu

2008-11-01

Ti/HA composites were successfully prepared by a powder metallurgy method and the effect of phase composition on the in vitro and in vivo bioactivity of the Ti/HA composites was investigated in the present study. The correlations between the in vitro and in vivo biological behaviors were highlighted. The results showed that the in vitro and in vivo bioactivity of the Ti/HA composites was dependent on their phase composition. The in vitro bioactivity of the Ti/HA composites was evaluated in simulated body fluid with ion concentrations similar to those of human plasma. After immersion in the simulated body fluid for a certain time, apatite precipitations formed on the surface of the composites with an initial titanium content of 50 and 70 wt.%, and no apatite was found on the surface of the composite with 30% titanium. Ti(2)O was responsible for the apatite formation on the surfaces of the composites. For in vivo analysis, Ti/HA cylinders were implanted in the metaphases of the rabbit femur. At the early stage of implantation, the new bone formed on the surface of the composite with 30% titanium was much less than that on the surfaces of the composites with 50% and 70% titanium. All the Ti/HA composites formed a chemical bone-bonding interface with the host bone by 6 months after implantation. The Ti/HA composites formed the bone-bonding interface with the surrounding bone through an apatite layer. The results in the present study suggested that the in vivo results agreed well with the in vitro results.

Response of Human Osteoblast to n-HA/PEEK—Quantitative Proteomic Study of Bio-effects of Nano-Hydroxyapatite Composite

Science.gov (United States)

Zhao, Minzhi; Li, Haiyun; Liu, Xiaochen; Wei, Jie; Ji, Jianguo; Yang, Shu; Hu, Zhiyuan; Wei, Shicheng

2016-03-01

Nano-sized hydroxyapatite (n-HA) is considered as a bio-active material, which is often mixed into bone implant material, polyetheretherketone (PEEK). To reveal the global protein expression modulations of osteoblast in response to direct contact with the PEEK composite containing high level (40%) nano-sized hydroxyapatite (n-HA/PEEK) and explain its comprehensive bio-effects, quantitative proteomic analysis was conducted on human osteoblast-like cells MG-63 cultured on n-HA/PEEK in comparison with pure PEEK. Results from quantitative proteomic analysis showed that the most enriched categories in the up-regulated proteins were related to calcium ion processes and associated functions while the most enriched categories in the down-regulated proteins were related to RNA process. This enhanced our understanding to the molecular mechanism of the promotion of the cell adhesion and differentiation with the inhibition of the cell proliferation on n-HA/PEEK composite. It also exhibited that although the calcium ion level of incubate environment hadn’t increased, merely the calcium fixed on the surface of material had influence to intracellular calcium related processes, which was also reflect by the higher intracellular Ca2+ concentration of n-HA/PEEK. This study could lead to more comprehensive cognition to the versatile biocompatibility of composite materials. It further proves that proteomics is useful in new bio-effect discovery.

Response of Human Osteoblast to n-HA/PEEK—Quantitative Proteomic Study of Bio-effects of Nano-Hydroxyapatite Composite

Science.gov (United States)

Zhao, Minzhi; Li, Haiyun; Liu, Xiaochen; Wei, Jie; Ji, Jianguo; Yang, Shu; Hu, Zhiyuan; Wei, Shicheng

2016-01-01

Nano-sized hydroxyapatite (n-HA) is considered as a bio-active material, which is often mixed into bone implant material, polyetheretherketone (PEEK). To reveal the global protein expression modulations of osteoblast in response to direct contact with the PEEK composite containing high level (40%) nano-sized hydroxyapatite (n-HA/PEEK) and explain its comprehensive bio-effects, quantitative proteomic analysis was conducted on human osteoblast-like cells MG-63 cultured on n-HA/PEEK in comparison with pure PEEK. Results from quantitative proteomic analysis showed that the most enriched categories in the up-regulated proteins were related to calcium ion processes and associated functions while the most enriched categories in the down-regulated proteins were related to RNA process. This enhanced our understanding to the molecular mechanism of the promotion of the cell adhesion and differentiation with the inhibition of the cell proliferation on n-HA/PEEK composite. It also exhibited that although the calcium ion level of incubate environment hadn’t increased, merely the calcium fixed on the surface of material had influence to intracellular calcium related processes, which was also reflect by the higher intracellular Ca2+ concentration of n-HA/PEEK. This study could lead to more comprehensive cognition to the versatile biocompatibility of composite materials. It further proves that proteomics is useful in new bio-effect discovery. PMID:26956660

The sunflower transcription factor HaHB11 improves yield, biomass and tolerance to flooding in transgenic Arabidopsis plants.

Science.gov (United States)

Cabello, Julieta V; Giacomelli, Jorge I; Piattoni, Claudia V; Iglesias, Alberto A; Chan, Raquel L

2016-03-20

HaHB11 is a member of the sunflower homeodomain-leucine zipper I subfamily of transcription factors. The analysis of a sunflower microarray hybridized with RNA from HaHB11-transformed leaf-disks indicated the regulation of many genes encoding enzymes from glycolisis and fermentative pathways. A 1300bp promoter sequence, fused to the GUS reporter gene, was used to transform Arabidopsis plants showing an induction of expression after flooding treatments, concurrently with HaHB11 regulation by submergence in sunflower. Arabidopsis transgenic plants expressing HaHB11 under the control of the CaMV 35S promoter and its own promoter were obtained and these plants exhibited significant increases in rosette and stem biomass. All the lines produced more seeds than controls and particularly, those of high expression level doubled seeds yield. Transgenic plants also showed tolerance to flooding stress, both to submergence and waterlogging. Carbohydrates contents were higher in the transgenics compared to wild type and decreased less after submergence treatments. Finally, transcript levels of selected genes involved in glycolisis and fermentative pathways as well as the corresponding enzymatic activities were assessed both, in sunflower and transgenic Arabidopsis plants, before and after submergence. Altogether, the present work leads us to propose HaHB11 as a biotechnological tool to improve crops yield, biomass and flooding tolerance. Copyright © 2016 Elsevier B.V. All rights reserved.

The effect of partially stabilized zirconia on the biological properties of HA/HDPE composites in vitro.

Science.gov (United States)

Sadi, A Yari; Shokrgozar, M A; Homaeigohar, S Sh; Hosseinalipour, M; Khavandi, A; Javadpour, J

2006-05-01

The effect of partially stabilized zirconia (PSZ) on the biological properties of the hyroxyapatite - high density polyethylene (HA/HDPE) composites was studied by investigating the simultaneous effect of hydroxyapatite and PSZ volume fractions on the in vitro response of human osteoblast cells. The biocompatibility of composite samples with different volume fraction of HA and PSZ powders was assessed by proliferation, alkaline phosphatase (ALP) and cell attachment assays on the osteoblast cell line (G-292) in different time periods. The effect of composites on the behavior of G-292 cells was compared with those of HDPE and TPS (Tissue Culture Poly Styrene as negative control) samples. Results showed a higher proliferation rate of G-292 cells in the presence of composite samples as compared to the HDPE sample after 7 and 14 days of incubation period. ALP production rate in all composite samples was higher than HDPE and TPS samples. The number of adhered cells on the composite samples was higher than the number adhered on the HDPE and TPS samples after the above mentioned incubation periods. These findings indicates that the addition of PSZ does not have any adverse affect on the biocompatibility of HA/HDPE composites. In fact in some experiments PSZ added HA/HDPE composites performed better in proliferation, differentiation and attachment of osteoblastic cells.

Trichomonas vaginalis Induces SiHa Cell Apoptosis by NF-κB Inactivation via Reactive Oxygen Species

Science.gov (United States)

Quan, Juan-Hua; Kang, Byung-Hun; Yang, Jung-Bo; Rhee, Yun-Ee; Noh, Heung-Tae; Choi, In-Wook; Cha, Guang-Ho; Yuk, Jae-Min

2017-01-01

Trichomonas vaginalis induces apoptosis in host cells through various mechanisms; however, little is known about the relationship between apoptosis, reactive oxygen species (ROS), and NF-κB signaling pathways in the cervical mucosal epithelium. Here, we evaluated apoptotic events, ROS production, and NF-κB activity in T. vaginalis-treated cervical mucosal epithelial SiHa cells, with or without specific inhibitors, using fluorescence microscopy, DNA fragmentation assays, subcellular fractionation, western blotting, and luciferase reporter assay. SiHa cells treated with live T. vaginalis at a multiplicity of infection of 5 (MOI 5) for 4 h produced intracellular and mitochondrial ROS in a parasite-load-dependent manner. Incubation with T. vaginalis caused DNA fragmentation, cleavage of caspase 3 and PARP, and release of cytochrome c into the cytoplasm. T. vaginalis-treated SiHa cells showed transient early NF-κB p65 nuclear translocation, which dramatically dropped at 4 h after treatment. Suppression of NF-κB activity was dependent on parasite burden. However, treatment with the ROS scavenger, N-acetyl-C-cysteine (NAC), reversed the effect of T. vaginalis on apoptosis and NF-κB inactivation in SiHa cells. Taken together, T. vaginalis induces apoptosis in human cervical mucosal epithelial cells by parasite-dose-dependent ROS production through an NF-κB-regulated, mitochondria-mediated pathway. PMID:29410962

Trichomonas vaginalis Induces SiHa Cell Apoptosis by NF-κB Inactivation via Reactive Oxygen Species

Directory of Open Access Journals (Sweden)

Juan-Hua Quan

2017-01-01

Full Text Available Trichomonas vaginalis induces apoptosis in host cells through various mechanisms; however, little is known about the relationship between apoptosis, reactive oxygen species (ROS, and NF-κB signaling pathways in the cervical mucosal epithelium. Here, we evaluated apoptotic events, ROS production, and NF-κB activity in T. vaginalis-treated cervical mucosal epithelial SiHa cells, with or without specific inhibitors, using fluorescence microscopy, DNA fragmentation assays, subcellular fractionation, western blotting, and luciferase reporter assay. SiHa cells treated with live T. vaginalis at a multiplicity of infection of 5 (MOI 5 for 4 h produced intracellular and mitochondrial ROS in a parasite-load-dependent manner. Incubation with T. vaginalis caused DNA fragmentation, cleavage of caspase 3 and PARP, and release of cytochrome c into the cytoplasm. T. vaginalis-treated SiHa cells showed transient early NF-κB p65 nuclear translocation, which dramatically dropped at 4 h after treatment. Suppression of NF-κB activity was dependent on parasite burden. However, treatment with the ROS scavenger, N-acetyl-C-cysteine (NAC, reversed the effect of T. vaginalis on apoptosis and NF-κB inactivation in SiHa cells. Taken together, T. vaginalis induces apoptosis in human cervical mucosal epithelial cells by parasite-dose-dependent ROS production through an NF-κB-regulated, mitochondria-mediated pathway.

Fabrication and bioactivity behavior of HA/bioactive glass composites in the presence of calcium hexaboride

Energy Technology Data Exchange (ETDEWEB)

El-Bassyouni, Gehan T.; Beherei, Hanan H. [Biomaterials Dept., National Research Centre (NRC), Dokki, Cairo (Egypt); Mohamed, Khaled R., E-mail: kh_rezk1966@yahoo.com [Biomaterials Dept., National Research Centre (NRC), Dokki, Cairo (Egypt); Kenawy, Sayed H. [Ceramics Dept., National Research Centre (NRC), Dokki, Cairo (Egypt)

2016-06-01

In the current study, composites were prepared using both the synthesized nano-sized hydroxyapatite (HA), bioactive glass (BG) powders (obtained by the traditional melt-quenching route) together with the purchased nano-sized calcium hexaboride (CB) with different ratios and were fired at 1250 °C. The structure and composition of the solid reaction products were analyzed using X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy; scanning electron microscope (SEM) coupled with energy dispersive spectroscopy (EDS), transmission electron microscope (TEM) techniques and compressive strength. The mechanical testing was to designate the role of the CB in improving the mechanical property of the prepared composites. In vitro bioactivity of the prepared composites was assessed by soaking in the simulated body fluid (SBF) at 37 ± 0.5 °°C for 10 days. The effect of different ratios of the three components (CB, HA & BG) on the bioactivity properties was assessed to explore the possibility of enhancing such property to perform in vitro imitations of in vivo conditions in the future. It can be pointed out that the Si-HA content in the composition showed outstanding in vitro bioactivity than pure hydroxyapatite which could be attributed to the excellent bioactivity of the synthesized composites. - Highlights: • The prepared of nano-composites containing CB, HA and BG powders were achieved. • The addition of CB powder enhanced the compressive strength for all the composites. • The composites containing high BG and CB contents improved formation of bone-like apatite layer.

Fabrication and bioactivity behavior of HA/bioactive glass composites in the presence of calcium hexaboride

International Nuclear Information System (INIS)

El-Bassyouni, Gehan T.; Beherei, Hanan H.; Mohamed, Khaled R.; Kenawy, Sayed H.

2016-01-01

In the current study, composites were prepared using both the synthesized nano-sized hydroxyapatite (HA), bioactive glass (BG) powders (obtained by the traditional melt-quenching route) together with the purchased nano-sized calcium hexaboride (CB) with different ratios and were fired at 1250 °C. The structure and composition of the solid reaction products were analyzed using X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy; scanning electron microscope (SEM) coupled with energy dispersive spectroscopy (EDS), transmission electron microscope (TEM) techniques and compressive strength. The mechanical testing was to designate the role of the CB in improving the mechanical property of the prepared composites. In vitro bioactivity of the prepared composites was assessed by soaking in the simulated body fluid (SBF) at 37 ± 0.5 °°C for 10 days. The effect of different ratios of the three components (CB, HA & BG) on the bioactivity properties was assessed to explore the possibility of enhancing such property to perform in vitro imitations of in vivo conditions in the future. It can be pointed out that the Si-HA content in the composition showed outstanding in vitro bioactivity than pure hydroxyapatite which could be attributed to the excellent bioactivity of the synthesized composites. - Highlights: • The prepared of nano-composites containing CB, HA and BG powders were achieved. • The addition of CB powder enhanced the compressive strength for all the composites. • The composites containing high BG and CB contents improved formation of bone-like apatite layer.

Haïti-Quisqueya: une double insularité

Directory of Open Access Journals (Sweden)

Jean-Marie THÉODAT

1998-09-01

Full Text Available Deux pays se partagent une île coupée par une frontière méridienne de plus de 300 km: Haïti à l'ouest, Quisqueya à l'est. Cette coupure est l'héritage du partage colonial de l'île entre la France et l'Espagne, et le résultat de la dynamique spatiale contrastée des deux nations qui y sont nées et qui ont eu recours à des principes d'encadrement distincts.De là un sentiment de double insularité, chaque pays se comportant vis-à-vis de son voisin comme s'ils étaient situés sur deux îles séparées.

The hormesis effect of plasma-elevated intracellular ROS on HaCaT cells

Science.gov (United States)

Szili, Endre J.; Harding, Frances J.; Hong, Sung-Ha; Herrmann, Franziska; Voelcker, Nicolas H.; Short, Robert D.

2015-12-01

We have examined the link between ionized-gas plasma delivery of reactive oxygen species (ROS) to immortalized keratinocyte (HaCaT) cells and cell fate, defined in terms of cell viability versus death. Phospholipid vesicles were used as cell mimics to measure the possible intracellular ROS concentration, [ROSi], delivered by various plasma treatments. Cells were exposed to a helium cold atmospheric plasma (CAP) jet for different plasma exposure times (5-60 s) and gas flow rates (50-1000 ml min-1). Based upon the [ROSi] data we argue that plasma-generated ROS in the cell culture medium can readily diffuse into real cells. Plasma exposure that equated to an [ROSi] in the range of 3.81  ×  10-10-9.47  ×  10-8 M, measured at 1 h after the plasma exposure, resulted in increased cell viability at 72 h; whereas a higher [ROSi] at 1 h decreased cell viability after 72 h of culture. This may be because of the manner in which the ROS are delivered by the plasma: HaCaT cells better tolerate a low ROS flux over an extended plasma exposure period of 1 min, compared to a high flux delivered in a few seconds, although the final [ROSi] may be the same. Our results suggest that plasma stimulation of HaCaT cells follows the principle of hormesis.

Bone Regeneration Using a Mixture of Silicon-Substituted Coral HA and β-TCP in a Rat Calvarial Bone Defect Model

Directory of Open Access Journals (Sweden)

Jiyeon Roh

2016-02-01

Full Text Available The demand of bone graft materials has been increasing. Among various origins of bone graft materials, natural coral composed of up to 99% calcium carbonate was chosen and converted into hydroxyapatite (HA; silicon was then substituted into the HA. Then, the Si-HA was mixed with β-tricalcium phosphate (TCP in the ratios 100:0 (S100T0, 70:30 (S70T30, 60:40 (S60T40, and 50:50 (S50T50. The materials were implanted for four and eight weeks in a rat calvarial bone defect model (8 mm. The MBCPTM (HA:β-TCP = 60:40, Biomatalante, Vigneux de Bretagne, France was used as a control. After euthanasia, the bone tissue was analyzed by making histological slides. From the results, S60T40 showed the fastest bone regeneration in four weeks (p < 0.05. In addition, S60T40, S50T50, and MBCPTM showed significant new bone formation in eight weeks (p < 0.05. In conclusion, Si-HA/TCP showed potential as a bone graft material.

Clinical significance of combined determination of the changes of the serum ADA, HA and PIIIP levels in patients with hepatitis B

International Nuclear Information System (INIS)

Wang Xiaoyan

2009-01-01

Objective: To explore the clinical significance of the changes of serum ADA, HA and PIIIP levels in patients with hepatitis B. Methods: Serum adenosine deaminase (ADA) (with biochemistry) and HA PIIIP (with RIA) levels were determined in 102 patients with hepatitis B of various stages and 35 controls. Results: Serum ADA, HA and PIIIP levels in patients with hepatitis B were significantly higher than those in controls (P<0.01). Particularly in patients with cirrhosis (n=13) (P<0.001). Conclusion: The serum ADA, HA and PIIIP levels were closely related to the diseases process of hepatitis B and were of great clinical values. (authors)

The Effect of Titanium Dioxide (TiO2) Nanoparticles on Hydroxyapatite (HA)/TiO2 Composite Coating Fabricated by Electrophoretic Deposition (EPD)

Science.gov (United States)

Amirnejad, M.; Afshar, A.; Salehi, S.

2018-05-01

Composite coatings of Hydroxyapatite (HA) with ceramics, polymers and metals are used to modify the surface structure of implants. In this research, HA/TiO2 composite coating was fabricated by electrophoretic deposition (EPD) on 316 stainless steel substrate. HA/TiO2 composite coatings with 5, 10 and 20 wt.% of TiO2, deposited at 40 V and 90 s as an optimum condition. The samples coated at this condition led to an adherent, continuous and crack-free coating. The influence of TiO2 content was studied by performing different characterization methods such as scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy (EDS), corrosion resistance in simulated body fluid (SBF), coating's dissolution rate in physiological solution and bond strength to the substrate. The results showed that the higher amount of TiO2 in the composite coating led to increase in bond strength of coating to stainless steel substrate from 3 MPa for HA coating to 5.5 MPa for HA-20 wt.% TiO2 composite coating. In addition, it caused to reduction of corrosion current density of samples in the SBF solution from 18.92 μA/cm2 for HA coating to 6.35 μA/cm2 for HA-20 wt.% TiO2 composite coating.

The Effect of Titanium Dioxide (TiO2) Nanoparticles on Hydroxyapatite (HA)/TiO2 Composite Coating Fabricated by Electrophoretic Deposition (EPD)

Science.gov (United States)

Amirnejad, M.; Afshar, A.; Salehi, S.

2018-04-01

Composite coatings of Hydroxyapatite (HA) with ceramics, polymers and metals are used to modify the surface structure of implants. In this research, HA/TiO2 composite coating was fabricated by electrophoretic deposition (EPD) on 316 stainless steel substrate. HA/TiO2 composite coatings with 5, 10 and 20 wt.% of TiO2, deposited at 40 V and 90 s as an optimum condition. The samples coated at this condition led to an adherent, continuous and crack-free coating. The influence of TiO2 content was studied by performing different characterization methods such as scanning electron microscopy (SEM), energy dispersive x-ray spectroscopy (EDS), corrosion resistance in simulated body fluid (SBF), coating's dissolution rate in physiological solution and bond strength to the substrate. The results showed that the higher amount of TiO2 in the composite coating led to increase in bond strength of coating to stainless steel substrate from 3 MPa for HA coating to 5.5 MPa for HA-20 wt.% TiO2 composite coating. In addition, it caused to reduction of corrosion current density of samples in the SBF solution from 18.92 μA/cm2 for HA coating to 6.35 μA/cm2 for HA-20 wt.% TiO2 composite coating.

In Vitro Degradation of PHBV Scaffolds and nHA/PHBV Composite Scaffolds Containing Hydroxyapatite Nanoparticles for Bone Tissue Engineering

Directory of Open Access Journals (Sweden)

Naznin Sultana

2012-01-01

Full Text Available This paper investigated the long-term in vitro degradation properties of scaffolds based on biodegradable polymers and osteoconductive bioceramic/polymer composite materials for the application of bone tissue engineering. The three-dimensional porous scaffolds were fabricated using emulsion-freezing/freeze-drying technique using poly(hydroxybutyrate-co-hydroxyvalerate (PHBV which is a natural biodegradable and biocompatible polymer. Nanosized hydroxyapatite (nHA particles were successfully incorporated into the PHBV scaffolds to render the scaffolds osteoconductive. The PHBV and nHA/PHBV scaffolds were systematically evaluated using various techniques in terms of mechanical strength, porosity, porous morphology, and in vitro degradation. PHBV and nHA/PHBV scaffolds degraded over time in phosphate-buffered saline at 37°C. PHBV polymer scaffolds exhibited slow molecular weight loss and weight loss in the in vitro physiological environment. Accelerated weight loss was observed in nHA incorporated PHBV composite scaffolds. An increasing trend of crystallinity was observed during the initial period of degradation time. The compressive properties decreased more than 40% after 5-month in vitro degradation. Together with interconnected pores, high porosity, suitable mechanical properties, and slow degradation profile obtained from long-term degradation studies, the PHBV scaffolds and osteoconductive nHA/PHBV composite scaffolds showed promises for bone tissue engineering application.

A Human Clinical, Histological, Histomorphometrical, and Radiographical Study on Biphasic HA-Beta-TCP 30/70 in Maxillary Sinus Augmentation.

Science.gov (United States)

Mangano, Carlo; Sinjari, Bruna; Shibli, Jamil A; Mangano, Francesco; Hamisch, Sabine; Piattelli, Adriano; Perrotti, Vittoria; Iezzi, Giovanna

2015-06-01

By mixing hydroxyapatite (HA) and tricalcium phosphate (TCP), biphasic calcium phosphate ceramics can be obtained, and by varying their ratio it is possible to tailor the characteristics of the biomaterial. The aim of the present human study was to evaluate the histological and radiographical aspects of bone formation in maxillary sinus augmentation using a 30/70 HA-beta-TCP with a reticular structure. A total of 12 patients, undergoing two-stage sinus augmentation procedure using HA-beta-TCP at a ratio of 30/70, were included in the present study. After a 6-month healing period, during implant insertion, radiographical analysis was performed, and then the bone core biopsies were harvested and processed for histology. At radiographic evaluation, the bone gain was on average 6.85 ± 0.60 mm. HA-beta-TCP 30/70 appeared to be lined by newly formed bone, with no gaps at the interface. The histomorphometric analysis revealed 26 ± 2% of residual grafted biomaterial, 29 ± 3% of newly formed bone, and 45 ± 2% of marrow spaces. The present results indicate histologically the high biocompatibility and osteoconductivity of HA-beta-TCP 30/70, and clinically its successful use for sinus augmentation procedures. © 2013 Wiley Periodicals, Inc.

In vitro study of RRS HA injectable mesotherapy/biorevitalization product on human skin fibroblasts and its clinical utilization.

Science.gov (United States)

Deglesne, Pierre-Antoine; Arroyo, Rodrigo; Ranneva, Evgeniya; Deprez, Philippe

2016-01-01

Mesotherapy/biorevitalization with hyaluronic acid (HA) is a treatment approach currently used for skin rejuvenation. Various products with a wide range of polycomponent formulations are available on the market. Most of these formulations contain noncross-linked HA in combination with a biorevitalization cocktail, formed by various amounts of vitamins, minerals, amino acids, nucleotides, coenzymes, and antioxidants. Although ingredients are very similar among the different products, in vitro and clinical effects may vary substantially. There is a real need for better characterization of these products in terms of their action on human skin or in vitro skin models. In this study, we analyzed the effect of the RRS(®) (Repairs, Refills, Stimulates) HA injectable medical device on human skin fibroblasts in vitro. Skin fibroblast viability and its capacity to induce the production of key extracellular matrix were evaluated in the presence of different concentrations of RRS HA injectable. Viability was evaluated through colorimetric MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, and key extracellular matrix genes, type I collagen and elastin, were quantified by quantitative polymerase chain reaction. Results demonstrated that RRS HA injectable could promote human skin fibroblast viability (+15%) and increase fibroblast gene expression of type I collagen and elastin by 9.7-fold and 14-fold in vitro, respectively. These results demonstrate that mesotherapy/biorevitalization products can, at least in vitro, effectively modulate human skin fibroblasts.

Technology transfer and scale-up of the Flublok recombinant hemagglutinin (HA) influenza vaccine manufacturing process.

Science.gov (United States)

Buckland, Barry; Boulanger, Robert; Fino, Mireli; Srivastava, Indresh; Holtz, Kathy; Khramtsov, Nikolai; McPherson, Clifton; Meghrous, Jamal; Kubera, Paul; Cox, Manon M J

2014-09-22

Multiple different hemagglutinin (HA) protein antigens have been reproducibly manufactured at the 650L scale by Protein Sciences Corporation (PSC) based on an insect cell culture with baculovirus infection. Significantly, these HA protein antigens were produced by the same Universal Manufacturing process as described in the biological license application (BLA) for the first recombinant influenza vaccine approved by the FDA (Flublok). The technology is uniquely designed so that a change in vaccine composition can be readily accommodated from one HA protein antigen to another one. Here we present a vaccine candidate to combat the recently emerged H7N9 virus as an example starting with the genetic sequence for the required HA, creation of the baculovirus and ending with purified protein antigen (or vaccine component) at the 10L scale accomplished within 38 days under GMP conditions. The same process performance is being achieved at the 2L, 10L, 100L, 650L and 2500L scale. An illustration is given of how the technology was transferred from the benchmark 650L scale facility to a retrofitted microbial facility at the 2500L scale within 100 days which includes the time for facility engineering changes. The successful development, technology transfer and scale-up of the Flublok process has major implications for being ready to make vaccine rapidly on a worldwide scale as a defense against pandemic influenza. The technology described does not have the same vulnerability to mutations in the egg adapted strain, and resulting loss in vaccine efficacy, faced by egg based manufacture. Copyright © 2014 Elsevier Ltd. All rights reserved.

The hot deformation behavior and microstructure evolution of HA/Mg-3Zn-0.8Zr composites for biomedical application.

Science.gov (United States)

Liu, Debao; Liu, Yichi; Zhao, Yue; Huang, Y; Chen, Minfang

2017-08-01

The hot deformation behavior of nano-sized hydroxylapatite (HA) reinforced Mg-3Zn-0.8Zr composites were performed by means of Gleeble-1500D thermal simulation machine in a temperature range of 523-673K and a strain rate range of 0.001-1s -1 , and the microstructure evolution during hot compression deformation were also investigated. The results show that the flow stress increases increasing strain rates at a constant temperature, and decreases with increasing deforming temperatures at a constant strain rate. Under the same processing conditions, the flow stresses of the 1HA/Mg-3Zn-0.8Zr specimens are higher than those of the Mg-3Zn-0.8Zr alloy specimens, and the difference is getting closer with increasing deformation temperature. The hot deformation behaviors of Mg-3Zn-0.8Zr and 1HA/Mg-3Zn-0.8Zr can be described by constitutive equation of hyperbolic sine function with the hot deformation activation energy being 124.6kJ/mol and 125.3kJ/mol, respectively. Comparing with Mg-3Zn-0.8Zr alloy, the instability region in the process map of 1HA/Mg-3Zn-0.8Zr expanded to a bigger extent at the same conditions. The optimum process conditions of 1HA/Mg-3Zn-0.8Zr composite is concluded as between the temperature window of 573-623K with a strain rate range of 0.001-0.1s -1 . A higher volume fraction and smaller grain size of dynamic recrystallization (DRX) grains was observed in 1HA/Mg-3Zn-0.8Zr specimens after the hot compression deformation compared with Mg-3Zn-0.8Zr alloy, which was ascribed to the presence of the HA particles that play an important role in particle-stimulated nucleation (PSN) mechanism and can effectively hinder the migration of interfaces. Copyright © 2017 Elsevier B.V. All rights reserved.

Polycystic Ovarian Syndrome: Is It Time to Rename PCOS to HA-PODS?

Science.gov (United States)

Khadilkar, Suvarna Satish

2016-04-01

The term polycystic ovarian syndrome (PCOS) came into existence 80 years ago. Pathophysiology of PCOS remains ill understood despite extensive research in this field. It is now accepted that the manifestations of PCOS are not confined to the reproductive dysfunction, and there are endocrine-metabolic implications to PCOS with several consequences to female health. PCOS is a misnomer as ovaries do not contain epithelial cysts, but they are actually antral follicles. Moreover, the name PCOS neither reflects the hyperandrogenism which is essential for diagnosis nor the metabolic derangements. While various authors have expressed the need for change of the name, a suitable new option has not yet been established. This review aims to analyse the current understanding of pathophysiology of PCOS and addresses to the controversies associated with its diagnosis and nomenclature. The name "Hyperandrogenic Persistent Ovulatory Dysfunction Syndrome or HA-PODS" is proposed here to overcome diagnostic pitfalls of previous nomenclature. This new name will help formulate appropriate treatment and promote consistency in research as well. Further categorizations of HA-PODS are also discussed in the article.

Investigation of morphology and bioactive properties of composite coating of HA/vinyl acetate on pure titanium

International Nuclear Information System (INIS)

Afshar, Abdollahe; Yousefpour, Mardali; Xiudong, Yang; Li Xudong; Yang Bangcheng; Wu Yao; Chen Jiyong; Zhang Xingdong

2006-01-01

Electrochemical co-deposition approach was expanded to prepare composite bio-ceramic coating of hydroxyapatite (HA)/polyvinyl acetate on the surface of titanium. The role is to improve the bioactive and crystallization properties. The results of XRD, XPS, SEM and TEM characterization showed that by increasing amount of vinyl acetate in the composite bio-ceramic coating before and after immersing in the simulated body fluid (SBF), an oriented growth of HA planes on the (0 0 2) direction had been observed on titanium substrate. Also significant surface morphology changes were obtained

Structure and transcription of the Helicoverpa armigera densovirus (HaDV2) genome and its expression strategy in LD652 cells.

Science.gov (United States)

Xu, Pengjun; Graham, Robert I; Wilson, Kenneth; Wu, Kongming

2017-02-07

Densoviruses (DVs) are highly pathogenic to their hosts. However, we previously reported a mutualistic DV (HaDV2). Very little was known about the characteristics of this virus, so herein we undertook a series of experiments to explore the molecular biology of HaDV2 further. Phylogenetic analysis showed that HaDV2 was similar to members of the genus Iteradensovirus. However, compared to current members of the genus Iteradensovirus, the sequence identity of HaDV2 is less than 44% at the nucleotide-level, and lower than 36, 28 and 19% at the amino-acid-level of VP, NS1 and NS2 proteins, respectively. Moreover, NS1 and NS2 proteins from HaDV2 were smaller than those from other iteradensoviruses due to their shorter N-terminal sequences. Two transcripts of about 2.2 kb coding for the NS proteins and the VP proteins were identified by Northern Blot and RACE analysis. Using specific anti-NS1 and anti-NS2 antibodies, Western Blot analysis revealed a 78 kDa and a 48 kDa protein, respectively. Finally, the localization of both NS1 and NS2 proteins within the cell nucleus was determined by using Green Fluorescent Protein (GFP) labelling. The genome organization, terminal hairpin structure, transcription and expression strategies as well as the mutualistic relationship with its host, suggested that HaDV2 was a novel member of the genus Iteradensovirus within the subfamily Densovirinae.

Euroveokid soosivad Venemaal käies üha enam Via Hanseaticat / Madis Aesma

Index Scriptorium Estoniae

Aesma, Madis

2005-01-01

Ajal. Postimees lüh. Pärast Balti riikide ühinemist Euroopa Liiduga kasutavad välismaa veoautojuhid üha enam Euroopast Venemaale sõitmiseks Valga-Tartu-Jõhvi-Narva maanteed, mis moodustab Via Hanseatica ehk Hansatee Eesti osa. Lisa: Via Hanseatica. Kaart: Visa Hanseatica

La investigación en valor percibido desde el marketing

Directory of Open Access Journals (Sweden)

Irene Gil Saura

2008-06-01

Full Text Available Desde el año 2000, hemos visto multiplicarse los intentos por comprender el verdadero significado del valor percibido en la compra y consumo de productos y servicios. Lo cierto es que el valor es considerado hoy como el corazón de la aproximación al marketing. Pero también es cierto que su estudio ha llegado después del interés por otras dos variables clave como la calidad y la satisfacción, y que existe un cierto lamento en la doctrina por una escasa y deficiente comprensión de lo que es y lo que no es valor percibido. En este artículo pretendemos abordar la investigación del valor percibido, enunciando sus luces y sus sombras, y ofreciendo un marco conceptual comprensivo que sirva de referencia para las diferentes aportaciones recogidas en esta publicación.

Ley sinde: sinfonía de un conflicto en la era digital

Directory of Open Access Journals (Sweden)

Albert García

2011-06-01

Full Text Available Ahora que ha pasado más de un mes desde el inesperado giro que sufrió la “Ley Sinde” durante su tramitación en el Senado, puede que haya llegado el momento de hacer balance de su dilatadísimo parto legal, así como de los procesos socio-políticos que lo han acompañado. Puede que Spielberg nunca adquiera los derechos para llevar a la gran pantalla esta contienda digital; puede que, siguiendo la lógica temporalmente atolondrada de la red, nadie componga nunca odas a estos hechos, pero hay ciertos fenómenos sociales que no pueden ser olvidados, que no deben serlo. Valga, pues, nuestro particular análisis de esta polifonía social a modo de síntesis, recordatorio y reflexión. Ocupen sus localidades, se abre el telón.

Ley sinde: sinfonía de un conflicto en la era digital

Directory of Open Access Journals (Sweden)

Albert García

2011-12-01

Full Text Available Ahora que ha pasado más de un mes desde el inesperado giro que sufrió la “Ley Sinde” durante su tramitación en el Senado, puede que haya llegado el momento de hacer balance de su dilatadísimo parto legal, así como de los procesos socio-políticos que lo han acompañado. Puede que Spielberg nunca adquiera los derechos para llevar a la gran pantalla esta contienda digital; puede que, siguiendo la lógica temporalmente atolondrada de la red, nadie componga nunca odas a estos hechos, pero hay ciertos fenómenos sociales que no pueden ser olvidados, que no deben serlo. Valga, pues, nuestro particular análisis de esta polifonía social a modo de síntesis, recordatorio y reflexión. Ocupen sus localidades, se abre el telón.

Áreas metropolitanas:¿qué ha cambiado?: La experiencia de la Caracas Metropolitana

Directory of Open Access Journals (Sweden)

Sonia Barrios

2001-05-01

Full Text Available La nueva economía de la información y del conocimiento ha tenido tres decisivas implicaciones de orden territorial. Primero, ha acelerado el proceso de aglomeración urbana a escala planetaria. Segundo, ha provocado fuertes alteraciones en las redes urbanas de ámbito mundial, regional y nacional. Y tercero, ha impulsado la reestructuración interna de las áreas metropolitanas que se habían conformado durante la era industrial. Todo parece anticipar, por lo tanto, polarizaciones y fragmentaciones territoriales todavía más acentuadas que las conocidas en un pasado cercano. Tomando como referencia el caso de Caracas, se trata de averiguar en qué medida el desarrollo reciente de esta metrópoli sigue las grandes tendencias territoriales antes anotadas; y qué esfuerzos se están haciendo para ajustar sus instancias locales a las nuevas realidades urbanas. Como un recurso adicional, en distintos momentos se establecen comparaciones con Barcelona (España, considerada un ejemplo emblemático de las metrópolis de la era de la informaciónThe developments of new informational and knowledge-based economies have had three decisive implications at the territorial level. First, it has accelerated the urban agglomeration processes worldwide. Second, it has brought about substantial alterations in the existing global, regional and national urban networks. Finally, it has triggered the internal restructuring of former industrial-based metropolitan areas. All this seems to anticipate that territorial polarization and fragmentation will reach higher levels than those we have observed in the recent past. Taking the case of Caracas as a reference point, the intention is to find out to what extent the recent development of this metropolis follows the main territorial trends mentioned above. Interest is also placed in finding what steps are being taken in order to adjust its local levels of government to the new urban realities. In addition, comparisons are

(+)-Nootkatone inhibits tumor necrosis factor α/interferon γ-induced production of chemokines in HaCaT cells

Energy Technology Data Exchange (ETDEWEB)

Choi, Hyeon-Jae; Lee, Jin-Hwee [College of Pharmacy, Ajou University, Suwon 443-749 (Korea, Republic of); Jung, Yi-Sook, E-mail: yisjung@ajou.ac.kr [College of Pharmacy, Ajou University, Suwon 443-749 (Korea, Republic of); Research Institute of Pharmaceutical Sciences and Technology, Ajou University, Suwon 443-749 (Korea, Republic of)

2014-05-02

Highlights: • (+)-Nootkatone inhibits TNF-α/IFN-γ-induced TARC and MDC expression in HaCaT cells. • PKCζ, p38 MAPK, or NF-κB mediate TNF-α/IFN-γ-induced TARC and MDC expression. • (+)-Nootkatone inhibits TNF-α/IFN-γ-induced activation of PKCζ, p38 MAPK, or NF-κB. • (+)-Nootkatone suppresses chemokine expression by inhibiting of PKCζ and p38 pathways. - Abstract: Chemokines are important mediators of cell migration, and thymus and activation-regulated chemokine (TARC/CCL17) and macrophage-derived chemokine (MDC/CCL22) are well-known typical inflammatory chemokines involved in atopic dermatitis (AD). (+)-Nootkatone is the major component of Cyperus rotundus. (+)-Nootkatone has antiallergic, anti-inflammatory, and antiplatelet activities. The purpose of this study was to investigate the effect of (+)-nootkatone on tumor necrosis factor α (TNF-α)/interferon γ (IFN-γ)-induced expression of Th2 chemokines in HaCaT cells. We found that (+)-nootkatone inhibited the TNF-α/IFN-γ-induced expression of TARC/CCL17 and MDC/CCL22 mRNA in HaCaT cells. It also significantly inhibited TNF-α/IFN-γ-induced activation of nuclear factor kappa B (NF-κB), p38 mitogen-activated protein kinase (MAPK), and protein kinase Cζ (PKCζ). Furthermore, we showed that PKCζ and p38 MAPK contributed to the inhibition of TNF-α/IFN-γ-induced TARC/CCL17 and MDC/CCL22 expression by blocking IκBα degradation in HaCaT cells. Taken together, these results suggest that (+)-nootkatone may suppress TNF-α/IFN-γ-induced TARC/CCL17 and MDC/CCL22 expression in HaCaT cells by inhibiting of PKCζ and p38 MAPK signaling pathways that lead to activation of NF-κB. We propose that (+)-nootkatone may be a useful therapeutic candidate for inflammatory skin diseases such as AD.

Ganándole la batalla al mal humor. Una propuesta didáctica

Directory of Open Access Journals (Sweden)

Sarah Díaz García

2012-08-01

Full Text Available En los últimos años, el aprendizaje de lenguas extranjeras ha llegado a ser una necesidad real tanto profesional como personal para todos nosotros como ciudadanos y como trabajadores. La enseñanza del español como lengua extranjera, que es el área de estudio en el que nos centraremos en el resto del presente artículo, ha evolucionado a medida que nuestra sociedad ha ido cambiando y adaptándose a los nuevos tiempos. Este cambio surgió con el Marco Común Europeo de Referencia para las Lenguas (MCER;[1] pasamos de darle una máxima importancia a los aspectos puramente lingüísticos a abrirnos al hecho de que aprender un segundo idioma no es solo centrarnos en la gramática. La competencia comunicativa, por ejemplo, ha ido cobrando protagonismo en los últimos años; la idea de que un estudiante solo es competente en el uso de una lengua cuando utiliza la lingüística (pensemos en la gramática de forma correcta y siguiendo la norma ha caído en desaprobación para muchos. La realidad es que para que un alumno se considere competente en el uso de otra lengua que no sea la suya debe saber utilizar las técnicas y recursos pertinentes para poder comunicarse efectivamente con su interlocutor en un contexto real de habla. [1] El Marco Común Europeo de Referencia para las Lenguas surgía en el año 2001. Desde entonces la enseñanza y el aprendizaje de lenguas extranjeras dio un cambio radical respecto a la metodología y la evaluación.

Reflexiones sobre la evolución legal de la libertad de expresión en Canadá y Colombia en el contexto de la consolidación democrática

Directory of Open Access Journals (Sweden)

Mauricio Beltrán-Cristancho

2011-05-01

Full Text Available Este artículo intenta, mediante la identificación de patrones históricos y decisiones jurídicas, comprender hasta dónde ha llegado el compromiso con la libertad de expresión en ambas naciones y las dificultades que de acuerdo a la experiencia canadiense está enfrentando el Estado colombiano, que todavía no ha logrado asegurar el monopolio de la fuerza dentro de su propio territorio para superar el primer escollo en su aspiración de consolidar la democracia. Cualquier intento por comparar el ejercicio de libertades en dos países con tradiciones jurídicas tan diferentes enfrenta dificultades apenas obvias. Cuando el tema es la libertad de expresión y uno de los Estados no ha podido obtener, después de 200 años de independencia, la estabilidad y seguridad que se requieren para asegurar un desarrollo económico sostenible, el ejercicio de comparación se complica y se hace más atractivo a la vez. Las historias de Canadá y Colombia son muy diferentes, aunque en el siglo XXI los principios de sus sistemas político y económico sean iguales. El compromiso de practicar la democracia y el buen gobierno, y de imponer el respeto a los derechos humanos, por lo menos formalmente, lo comparten hoy Colombia y Canadá. Esta es la motivación fundamental de este análisis.

HaCaT Keratinocytes and Primary Epidermal Keratinocytes Have Different Transcriptional Profiles of Cornified Envelope-Associated Genes to T Helper Cell Cytokines

Science.gov (United States)

Seo, Min-Duk; Kang, Tae Jin; Lee, Chang Hoon; Lee, Ai-Young; Noh, Minsoo

2012-01-01

HaCaT cells are the immortalized human keratinocytes and have been extensively used to study the epidermal homeostasis and its pathophysiology. T helper cells play a role in various chronic dermatological conditions and they can affect skin barrier homeostasis. To evaluate whether HaCaT cells can be used as a model cell system to study abnormal skin barrier development in various dermatologic diseases, we analyzed the gene expression profile of epidermal differentiation markers of HaCaT cells in response to major T helper (Th) cell cytokines, such as IFNγ, IL-4, IL-17A and IL-22. The gene transcriptional profile of cornified envelope-associated proteins, such as filaggrin, loricrin, involucrin and keratin 10 (KRT10), in HaCaT cells was generally different from that in normal human keratinocytes (NHKs). This suggests that HaCaT cells have a limitation as a model system to study the pathophysiological mechanism associated with the Th cell cytokine-dependent changes in cornified envelope-associated proteins which are essential for normal skin barrier development. In contrast, the gene transcription profile change of human β2-defensin (HBD2) in response to IFNγ, IL-4 or IL-17A in HaCaT cells was consistent with the expression pattern of NHKs. IFNγ also up-regulated transglutaminase 2 (TGM2) gene transcription in both HaCaT cells and NHKs. As an alternative cell culture system for NHKs, HaCaT cells can be used to study molecular mechanisms associated with abnormal HBD2 and TGM2 expression in response to IFNγ, IL-4 or IL-17A. PMID:24116291

Extraction of hyaluronic acid (HA) from rooster comb and characterization using flow field-flow fractionation (FlFFF) coupled with multiangle light scattering (MALS).

Science.gov (United States)

Kang, Dong Young; Kim, Won-Suk; Heo, In Sook; Park, Young Hun; Lee, Seungho

2010-11-01

Hyaluronic acid (HA) was extracted in a relatively large scale from rooster comb using a method similar to that reported previously. The extraction method was modified to simplify and to reduce time and cost in order to accommodate a large-scale extraction. Five hundred grams of frozen rooster combs yielded about 500 mg of dried HA. Extracted HA was characterized using asymmetrical flow field-flow fractionation (AsFlFFF) coupled online to a multiangle light scattering detector and a refractive index detector to determine the molecular size, molecular weight (MW) distribution, and molecular conformation of HA. For characterization of HA, AsFlFFF was operated by a simplified two-step procedure, instead of the conventional three-step procedure, where the first two steps (sample loading and focusing) were combined into one to avoid the adsorption of viscous HA onto the channel membrane. The simplified two-step AsFlFFF yielded reasonably good separations of HA molecules based on their MWs. The weight average MW (M(w) ) and the average root-mean-square (RMS) radius of HA extracted from rooster comb were 1.20×10(6) and 94.7 nm, respectively. When the sample solution was filtered through a 0.45 μm disposable syringe filter, they were reduced down to 3.8×10(5) and 50.1 nm, respectively. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Injection of an antibody against a p21 c-Ha-ras protein inhibits cleavage in axolotl eggs.

OpenAIRE

Baltus, E; Hanocq-Quertier, J; Hanocq, F; Brachet, J

1988-01-01

The presence of a ras protein was demonstrated in cleaving axolotl eggs by selective immunoprecipitation with a polyclonal antibody against a peptide encoded by the c-Ha-ras oncogene, cellular homolog of the v-Ha-ras oncogene of Harvey rat sarcoma virus. Injection of this antibody into axolotl oocytes subjected to progesterone treatment does not prevent meiotic maturation. Injection of the same antibody into a blastomere of axolotl eggs at the 2- or 4-cell stage causes cleavage arrest in the ...

Dihydrotestosterone induces SREBP-1 expression and lipogenesis through the phosphoinositide 3-kinase/Akt pathway in HaCaT cells

Directory of Open Access Journals (Sweden)

Zhou Bing-rong

2012-11-01

Full Text Available Abstract Background The purpose of this study was to investigate the effects and mechanisms of dihydrotestosterone (DHT-induced expression of sterol regulatory element binding protein-1 (SREBP-1, and the synthesis and secretion of lipids, in HaCaT cells. HaCaT cells were treated with DHT and either the phosphoinositide 3-kinase inhibitor LY294002 or the extracellular-signal-regulated kinase (ERK inhibitor PD98059. Real time-PCR, Western blot, Oil Red staining and flow cytometry were employed to examine the mRNA and protein expressions of SREBP-1, the gene transcription of lipid synthesis, and lipid secretion in HaCaT cells. Findings We found that DHT upregulated mRNA and protein expressions of SREBP-1. DHT also significantly upregulated the transcription of lipid synthesis-related genes and increased lipid secretion, which can be inhibited by the addition of LY294002. Conclusions Collectively, these results indicate that DHT induces SREBP-1 expression and lipogenesis in HaCaT cells via activation of the phosphoinositide 3-kinase/Akt Pathway.

Bâtir un avenir pour Haïti grâce aux études supérieures | CRDI ...

International Development Research Centre (IDRC) Digital Library (Canada)

9 mai 2017 ... Image. Un membre du Groupe de Réflexion et d'Action pour une Haïti nouvelle. CRDI. Le tremblement de terre d'une magnitude de 7,0 qui a frappé Haïti en janvier 2010 a touché particulièrement durement les universités de ce pays. Les secousses sismiques ont détruit des immeubles, et quelque 100 000 ...

Supporting Timely Humanitarian Assistance/Disaster Relief (HA/DR) Decisions Through Geospatial Intelligence (GEOINT) and Geographical Information Systems (GIS) Tools

Science.gov (United States)

2014-05-22

APPENDIX D: JUBA HA/DR COP SHARED VIA A GOOGLE EARTH ® KML FILE ........... 60 APPENDIX E: GEOINT REFERENCE AIDS...2003 and 2012................. 51 Figure 13. JTF-Haiti (27 Jan 10): DPs, IDP groups, flood and landslide risks. ............................. 57...Figure 14. Juba HA/DR COP displayed in Google Earth ® ........................................................... 60 Figure 15. Overview of GEOINT data

Production of light fragments in hA collisions at high energies

International Nuclear Information System (INIS)

Braun, M.A.; Vechernin, V.V.

1988-12-01

Production of fast relativistic light fragments in hA collisions at high energies is considered. Direct coalescence of produced nucleons into fragments is shown to be the main mechanism for fragment production. The influence of the nuclear field is small and is not described by the well-known Butler-Pearson formulas. The coalescence coefficient strongly depends on the angle and on the behaviour of the fragment wave function at small internucleon distances. (author). 14 refs, 7 figs

Cobalt toxicity: Chemical and radiological combined effects on HaCaT keratinocyte cell line

International Nuclear Information System (INIS)

Sandre, C.; Moulin, C.; Bresson, C.; Gault, N.; Poncy, J. L.; Lefaix, J. L.

2010-01-01

Cobalt (Co) is an essential trace element well known as a constituent of vitamin B 12 , but different compounds of Co are also described as highly toxic and/or radio-toxic for individuals or the environment. In nuclear power plants, 58 Co and 60 Co are radioactive isotopes of cobalt present as activation products of stable Co and Ni used in alloys. Skin exposure is a current occupational risk in the hard metal and nuclear industries. As biochemical and molecular cobalt-induced toxicological mechanisms are not fully identified, we investigated cobalt toxicity in a model human keratinocyte cell line, HaCaT. In this study, we propose a model to determine the in vitro chemical impact on cell viability of a soluble form of cobalt (CoCl 2 ) with or without gamma-ray doses to mimic contamination by 60 Co, to elucidate the mechanisms of cobalt intracellular chemical and radiological toxicity. Intracellular cobalt concentration was determined after HaCaT cell contamination and chemical toxicity was evaluated in terms of cellular viability and clonogenic survival. We investigated damage to DNA in HaCaT cells by combined treatment with chemical cobalt and a moderate gamma-ray dose. Additive effects of cobalt and irradiation were demonstrated. The underlying mechanism of cobalt toxicity is not clearly established, but our results seem to indicate that the toxicity of Co(II) and of irradiation arises from production of reactive oxygen species. (authors)

Cobalt toxicity: Chemical and radiological combined effects on HaCaT keratinocyte cell line

Energy Technology Data Exchange (ETDEWEB)

Gault, N. [CEA Fontenay aux Roses, DSV/IRCM/SCSR/LRTS, 92265 Fontenay aux Rose (France); Sandre, C.; Moulin, B.; Bresson, C. [CEA, DEN, SECR, Laboratoire de Speciation des Radionucleides et des Molecules, F-91191 Gif-sur-Yvette (France); Poncy, J.L. [CEA Bruyeres Le Chatel, DSV/IRCM/SREIT/LRT, 91680 Bruyeres Le Chatel (France); Lefaix, J.L. [CEA Caen, DSV/IRCM/SRO/LARIA, 14070 Caen (France)

2010-07-01

Cobalt (Co) is an essential trace element well known as a constituent of vitamin B12, but different compounds of Co are also described as highly toxic and/or radio-toxic for individuals or the environment. In nuclear power plants, {sup 58}Co and {sup 60}Co are radioactive isotopes of cobalt present as activation products of stable Co and Ni used in alloys. Skin exposure is a current occupational risk in the hard metal and nuclear industries. As biochemical and molecular cobalt-induced toxicological mechanisms are not fully identified, we investigated cobalt toxicity in a model human keratinocyte cell line, HaCaT. In this study, we propose a model to determine the in vitro chemical impact on cell viability of a soluble form of cobalt (CoCl{sub 2}) with or without {gamma}-ray doses to mimic contamination by {sup 60}Co, to elucidate the mechanisms of cobalt intracellular chemical and radiological toxicity. Intracellular cobalt concentration was determined after HaCaT cell contamination and chemical toxicity was evaluated in terms of cellular viability and clonogenic survival. We investigated damage to DNA in HaCaT cells by combined treatment with chemical cobalt and a moderate {gamma}-ray dose. Additive effects of cobalt and irradiation were demonstrated. The underlying mechanism of cobalt toxicity is not clearly established, but our results seem to indicate that the toxicity of Co(II) and of irradiation arises from production of reactive oxygen species. (authors)

Cobalt toxicity: Chemical and radiological combined effects on HaCaT keratinocyte cell line

Energy Technology Data Exchange (ETDEWEB)

Sandre, C.; Moulin, C.; Bresson, C. [CEA Saclay, DEN, SECR, Lab Speciat Radionucleides and Mol, F-91191 Gif Sur Yvette (France); Gault, N. [CEA Fontenay Roses, DSV IRCM SCSR LRTS, F-92265 Fontenay Aux Roses (France); Poncy, J. L. [CEA Bruyeres Le Chatel, DSV IRCM SREIT LRT, F-91680 Bruyeres Le Chatel (France); Lefaix, J. L. [CEA Caen, DSV IRCM SRO LARIA, F-14070 Caen (France)

2010-07-01

Cobalt (Co) is an essential trace element well known as a constituent of vitamin B{sub 12}, but different compounds of Co are also described as highly toxic and/or radio-toxic for individuals or the environment. In nuclear power plants, {sup 58}Co and {sup 60}Co are radioactive isotopes of cobalt present as activation products of stable Co and Ni used in alloys. Skin exposure is a current occupational risk in the hard metal and nuclear industries. As biochemical and molecular cobalt-induced toxicological mechanisms are not fully identified, we investigated cobalt toxicity in a model human keratinocyte cell line, HaCaT. In this study, we propose a model to determine the in vitro chemical impact on cell viability of a soluble form of cobalt (CoCl{sub 2}) with or without gamma-ray doses to mimic contamination by {sup 60}Co, to elucidate the mechanisms of cobalt intracellular chemical and radiological toxicity. Intracellular cobalt concentration was determined after HaCaT cell contamination and chemical toxicity was evaluated in terms of cellular viability and clonogenic survival. We investigated damage to DNA in HaCaT cells by combined treatment with chemical cobalt and a moderate gamma-ray dose. Additive effects of cobalt and irradiation were demonstrated. The underlying mechanism of cobalt toxicity is not clearly established, but our results seem to indicate that the toxicity of Co(II) and of irradiation arises from production of reactive oxygen species. (authors)

Determination of Ca/P molar ratio in hydroxyapatite (HA) by X-ray fluorescence technique

International Nuclear Information System (INIS)

Scapin, Marcos A.; Guilhen, Sabine N.; Cotrim, Marycel E.B.; Pires, Maria Ap. F.

2015-01-01

Hydroxyapatite (HA) is a mineral composed of calcium phosphate employed for endodontics, restorative dentistry and other applications in orthopedics and prosthesis. Additionally, this biomaterial is an inexpensive but efficient adsorbent for the removal of heavy metals and other unwanted species of contaminated liquid effluents. This is especially interesting when low-cost effective remediation is required. A Ca / P molar ratio of 1.667 is consistent with the theoretical Ca / P ratio for calcium hydroxyapatite with a compositional formula of Ca 10 (PO 4 ) 6 (OH) 2 , which properties are well discussed in the literature. The aim of this work was to implement and validate a methodology for simultaneous determination of major and minor constituents in the hydroxyapatite (HA) as well as providing the Ca / P molar ratio. To accomplish these achievements, wavelength dispersive X-ray fluorescence spectroscopy (WDXRF) was applied. This is a non-destructive technique that requires no chemical treatment, enabling fast chemical analysis in a wide variety of samples, with no hazardous waste being generated as a result of the process of determination. A standard reference material from NIST (SRM 1400 – Bone Ash) was used to validate the methodology for the determination of magnesium, phosphorus, potassium, calcium, iron, zinc, strontium and the Ca / P ratio in HA samples by WDXRF. The Z-score test was applied as a statistical tool and showed that the calculated values were of less than 1.8 for all the measured analytes. (author)

Determination of Ca/P molar ratio in hydroxyapatite (HA) by X-ray fluorescence technique

Energy Technology Data Exchange (ETDEWEB)

Scapin, Marcos A.; Guilhen, Sabine N.; Cotrim, Marycel E.B.; Pires, Maria Ap. F., E-mail: mascapin@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

2015-07-01

Hydroxyapatite (HA) is a mineral composed of calcium phosphate employed for endodontics, restorative dentistry and other applications in orthopedics and prosthesis. Additionally, this biomaterial is an inexpensive but efficient adsorbent for the removal of heavy metals and other unwanted species of contaminated liquid effluents. This is especially interesting when low-cost effective remediation is required. A Ca / P molar ratio of 1.667 is consistent with the theoretical Ca / P ratio for calcium hydroxyapatite with a compositional formula of Ca{sub 10}(PO{sub 4}){sub 6}(OH){sub 2}, which properties are well discussed in the literature. The aim of this work was to implement and validate a methodology for simultaneous determination of major and minor constituents in the hydroxyapatite (HA) as well as providing the Ca / P molar ratio. To accomplish these achievements, wavelength dispersive X-ray fluorescence spectroscopy (WDXRF) was applied. This is a non-destructive technique that requires no chemical treatment, enabling fast chemical analysis in a wide variety of samples, with no hazardous waste being generated as a result of the process of determination. A standard reference material from NIST (SRM 1400 – Bone Ash) was used to validate the methodology for the determination of magnesium, phosphorus, potassium, calcium, iron, zinc, strontium and the Ca / P ratio in HA samples by WDXRF. The Z-score test was applied as a statistical tool and showed that the calculated values were of less than 1.8 for all the measured analytes. (author)

El estudiante como protagonista de su aprendizaje: la necesidad del uso del portafolio en enfermería dentro del contexto de educación superior

Directory of Open Access Journals (Sweden)

Eva García-Carpintero Blas

2015-06-01

Full Text Available Las reformas promovidas por el proceso Bolonia han implicado cambios en las formas de enseñar y aprender dentro del marco del Espacio Europeo de Educación Superior. Esta amplia remodelación trae consigo por un lado un cambio estructural y, por otro, una reconceptualización del proceso de aprendizaje apoyada en las teorías constructivistas, a través de estrategias metodológicas de construcción del conocimiento desarrolladas por los estudiantes. El objetivo del presente artículo es presentar una reflexión teórica acerca del uso del portafolio como recurso didáctico para la enseñanza y aprendizaje dentro de este contexto. Se ha observado que la implicación de los estudiantes en la identificación de los problemas a través del portafolio, facilita a los docentes una mayor comprensión de los procesos de aprendizaje de los estudiantes, así como una oportunidad para éstos de efectuar un aprendizaje más reflexivo, autónomo y participativo, tan importante en disciplinas eminentemente prácticas como la Enfermería.

El modelo de Súnion y el pronóstico de la educación permanente. Lo pendiente desde una doble perspectiva

Directory of Open Access Journals (Sweden)

Eva Gregori Giralt

2007-09-01

Full Text Available La idea de que el proceso de Bolonia y la reforma universitaria representan un cambio radical del modelo educativo y una auténtica renovación didáctica no halla su correspondencia necesaria en la teoría pedagógica de la educación superior. Conscientes de la importancia y la repercusión de esta nueva realidad, que es ya un hecho en la práctica cotidiana de las academias, el presente artículo intenta trazar un paralelismo con lo que aconteció en otros momentos clave de la pedagogía moderna. En la didáctica del siglo XX, la dimensión práctica de la enseñanza ha dejado faltas de un análisis teórico a no pocas iniciativas. Mediante el ejemplo de Súnion. Institució Cultural Catalana se considera hasta qué punto conviene mirar a la enseñanza primaria y secundaria para prever el futuro de la enseñanza superior y prepararse para un profundo replanteamiento tanto del aprendizaje y de lo aprendido como de las figuras del profesor y del estudiante.

In vitro study of RRS HA injectable mesotherapy/biorevitalization product on human skin fibroblasts and its clinical utilization

Directory of Open Access Journals (Sweden)

Deglesne PA

2016-02-01

Full Text Available Pierre-Antoine Deglesne,* Rodrigo Arroyo,* Evgeniya Ranneva, Philippe Deprez Research and Development, SKIN TECH PHARMA GROUP, Castelló d'Empúries, Spain  *These authors contributed equally to this work Abstract: Mesotherapy/biorevitalization with hyaluronic acid (HA is a treatment approach currently used for skin rejuvenation. Various products with a wide range of polycomponent formulations are available on the market. Most of these formulations contain noncross-linked HA in combination with a biorevitalization cocktail, formed by various amounts of vitamins, minerals, amino acids, nucleotides, coenzymes, and antioxidants. Although ingredients are very similar among the different products, in vitro and clinical effects may vary substantially. There is a real need for better characterization of these products in terms of their action on human skin or in vitro skin models. In this study, we analyzed the effect of the RRS® (Repairs, Refills, Stimulates HA injectable medical device on human skin fibroblasts in vitro. Skin fibroblast viability and its capacity to induce the production of key extracellular matrix were evaluated in the presence of different concentrations of RRS HA injectable. Viability was evaluated through colorimetric MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay, and key extracellular matrix genes, type I collagen and elastin, were quantified by quantitative polymerase chain reaction. Results demonstrated that RRS HA injectable could promote human skin fibroblast viability (+15% and increase fibroblast gene expression of type I collagen and elastin by 9.7-fold and 14-fold in vitro, respectively. These results demonstrate that mesotherapy/biorevitalization products can, at least in vitro, effectively modulate human skin fibroblasts.Keywords: mesotherapy, medical device, RRS, collagen, elastin, extracellular matrix

El influjo de María : relato sobre la inmigración japonesa y el desarrollo del capitalismo en la agricultura del Valle del Cauca

Directory of Open Access Journals (Sweden)

Germán Patiño Ossa

1992-01-01

Full Text Available Cincuenta años más tarde de la llegada de las primeras cinco familias de japoneses al puerto de Buenaventura, Eugenio Bamey dio fin a la elaboración de una obra en la que rastrea las posibles influencias de los diferentes grupos de inmigrantes en los hábitos alimentarios de la población vallecaucana. Al núcleo japonés, aunque le reconoce su destacado papel en la evolución de la técnica agricola regional, no le encuentra mayor significado como elemento aportante en las costumbres culinarias. Sin embargo, ya era frecuente en Cali el consumo popular de helado de soya, que se expende empacado en bolsas de polietileno, a bajos precios, en diferentes esquinas de la ciudad. ¿Y el seviche de camarón, que procede de la cocina japonesa, y que ha hecho su tránsito al valle caucano desde la costa peruana, no ha llegado para instalarse definitivamente como plato típico? Estos dos ejemplos muestran cómo la influencia cultural japonesa es más fuerte de lo que comúnmente se piensa y abarca territorios que van más allá de los habituales referentes a la actividad agropecuaria.

DEVOTEES, WANNABES Y PRETENDERS: PARAFILIAS VINCULADAS A LA DISCAPACIDAD

Directory of Open Access Journals (Sweden)

Carolina Mora

2016-01-01

Full Text Available Con el desarrollo de internet se ha ido haciendo evidente la vinculación entre discapacidad y sexualidad, en la que las designaciones “devotees”, “pretedenders” y “wannabes” (DPW juegan un rol central. Los devotees son individuos sanos que se sienten atraídos sexualmente hacia personas con discapacidad, generalmente con problemas de movilidad. Los pretenders son personas que actúan como si tuvieran una discapacidad imitando sus movimientos, usando muletas, sillas de ruedas, escayolas, etc. Por último, los wannabes desean ser discapacitados, algunos de ellos han llegado al extremo de hacerse amputaciones caseras cuando la medicina tradicional se ha negado a dar respuesta a su necesidad. La presente investigación documental indaga sobre cada uno de los miembros de la taxonomía DPW, presentando: a las hipótesis existentes con relación a su etiología; b su vinculación con otras entidades diagnósticas presentes en el DSM V; c se describen algunos de los tratamientos que se han empleado en su abordaje terapéutico, y d se mencionan las interrelaciones entre estas 3 categorías, dado que los limites entre ellas son muy flexibles.

Los Derechos Humanos Después del 11 de Septiembre

Directory of Open Access Journals (Sweden)

Jean Paul Marthoz

2003-01-01

Full Text Available La guerra con tra el terrorismo marca un cambio de paradigma y la afirmación de otra cosmovisión. Ciertos vaticinan que la democracia, tal como se vivía o se soñaba después del derrumbe del comunismo soviético, ha llegado a un peak y que el 11 de septiembre ha detenido el avance que se creía ir re vers ible del imperio de la ley y del derecho en el concierto de las naciones. La proliferación de medidas restrictivas o represivas, el recurso a la fraseología y la lógica de la guerra, el endurecimiento de una opinión pública angustiada e insegura, el re torno al discurso de la re al po li tik, han debilitado el movimiento de los derechos humanos, fragilizado sus argumentaciones y marginado sus acciones. Las ONG’s de derechos humanos tienen que acordarse que la batalla por los derechos no se hace únicamente sobre el terreno le gal y con las armas del derecho internacional. La lucha por los derechos humanos es también una batalla política, es decir, intelectual, que implica desarrollar cuadros interpretativos del mundo.

HOTEL MANDARIN ORIENTAL DE BARCELONA. OBRA DE INTERIORISMO Y DISEÑO DE MOBILIARIO DE PATRICIA URQUIOLA

Directory of Open Access Journals (Sweden)

Alicia Menendez Martinez

2013-07-01

Full Text Available La arquitectay diseñadora asturiana, afincada en Milán, Patricia Urquiola despliega en la obrade interiorismo integral realizada en el Hotel Mandarin Oriental de Barcelonaun universo de piezas y ambientes que permite un acercamiento al diseño másinternacional del siglo XXI. A través de este artículo, se pretende analizar lagran escenografía de lujo contenido que ha conseguido la autora y lasconsideraciones sobre diseño contemporáneo que salen al paso de este análisis.Conceptos como buen gusto, continuidad, refinamiento, esencialidad,sensibilidad o delicadeza se unen al de femineidad para volverse una constantea lo largo de estas páginas. El trabajo realizado por Urquiola en esteequipamiento hotelero de gran lujo pone de manifiesto su irrupción por méritospropios en el mundo del diseño contemporáneo internacional y la encumbra comoartista polifacética y multidisciplinar preparada para amoldarse a las másaltas exigencias del mercado. El interiorismo del Mandarin es la prueba de queUrquiola ha llegado a lo más alto para quedarse. A su formación hispano-italianase añade un punto de vista sorprendentemente nuevo, el de una mujer diseñadora.

Métodos de Tratamiento en los Tabacos Colombianos y su Influencia en el Contenido de Nicotina

Directory of Open Access Journals (Sweden)

Londoño G. Horacio

1939-12-01

Full Text Available “Nicotina" es el género botánico con que los científicos han designado una de las plantas más explotadas en el mundo industrial, que ha sido en todos los tiempos fuente de riqueza para los países que la producen y aun para aquellos que la importan, pero que, mediante su industrialización, obtienen de ella pingües beneficios. Entre nosotros ha dado origen a la verdadera industria autóctona y, tras ingentes luchas, ha llegado a ser explotada de una manera científica y con una organización que es envidiada por muchos países de ultramar. En Colombia, sin una verdadera clasificación científica de los tabacos, diferenciamos sin embargo, las cualidades de cada uno de ellos por medio de los procedimientos un poco empíricos, de distinguirlos de acuerdo con las regiones productoras. Como base de nuestra industria tabacalera, el tabaco Santander se ha impuesto definitivamente, debido a sus excelentes cualidades; le siguen en importancia los del Tolima, Valle, Cauca, Nariño, Huila y Antioquia, figurando en un renglón totalmente separado el tabaco de Bolívar, que muy poca o ninguna influencia tiene en el desarrollo de nuestra industria y que sólo constituye un renglón importante bajo el aspecto de exportación, ya que su precio sumamente bajo es el único que permite competir en los mercados extranjeros con calidades superiores, pero que económicamente no pueden ofrecerse en idénticas condiciones.

Caveolin-1 down-regulation is required for Wnt5a-Frizzled 2 signalling in Ha-RasV12 -induced cell transformation.

Science.gov (United States)

Lin, Hsiu-Kuan; Lin, Hsi-Hui; Chiou, Yu-Wei; Wu, Ching-Lung; Chiu, Wen-Tai; Tang, Ming-Jer

2018-05-01

Caveolin-1 (Cav1) is down-regulated during MK4 (MDCK cells harbouring inducible Ha-Ras V12 gene) transformation by Ha-Ras V12 . Cav1 overexpression abrogates the Ha-Ras V12 -driven transformation of MK4 cells; however, the targeted down-regulation of Cav1 is not sufficient to mimic this transformation. Cav1-silenced cells, including MK4/shCav1 cells and MDCK/shCav1 cells, showed an increased cell area and discontinuous junction-related proteins staining. Cellular and mechanical transformations were completed when MDCK/shCav1 cells were treated with medium conditioned by MK4 cells treated with IPTG (MK4+I-CM) but not with medium conditioned by MK4 cells. Nanoparticle tracking analysis showed that Ha-Ras V12 -inducing MK4 cells increased exosome-like microvesicles release compared with their normal counterparts. The cellular and mechanical transformation activities of MK4+I-CM were abolished after heat treatment and exosome depletion and were copied by exosomes derived from MK4+I-CM (MK4+I-EXs). Wnt5a, a downstream product of Ha-Ras V12 , was markedly secreted by MK4+I-CM and MK4+I-EXs. Suppression of Wnt5a expression and secretion using the porcupine inhibitor C59 or Wnt5a siRNA inhibited the Ha-Ras V12 - and MK4+I-CM-induced transformation of MK4 cells and MDCK/shCav1 cells, respectively. Cav1 down-regulation, either by Ha-Ras V12 or targeted shRNA, increased frizzled-2 (Fzd2) protein levels without affecting its mRNA levels, suggesting a novel role of Cav1 in negatively regulating Fzd2 expression. Additionally, silencing Cav1 facilitated the internalization of MK4+I-EXs in MDCK cells. These data suggest that Cav1-dependent repression of Fzd2 and exosome uptake is potentially relevant to its antitransformation activity, which hinders the activation of Ha-Ras V12 -Wnt5a-Stat3 pathway. Altogether, these results suggest that both decreasing Cav1 and increasing exosomal Wnt5a must be implemented during Ha-Ras V12 -driven cell transformation. © 2018 The Authors

Topical stabilized retinol treatment induces the expression of HAS genes and HA production in human skin in vitro and in vivo.

Science.gov (United States)

Li, Wen-Hwa; Wong, Heng-Kuan; Serrano, José; Randhawa, Manpreet; Kaur, Simarna; Southall, Michael D; Parsa, Ramine

2017-05-01

Skin Aging manifests primarily with wrinkles, dyspigmentations, texture changes, and loss of elasticity. During the skin aging process, there is a loss of moisture and elasticity in skin resulting in loss of firmness finally leading to skin sagging. The key molecule involved in skin moisture is hyaluronic acid (HA), which has a significant water-binding capacity. HA levels in skin decline with age resulting in decrease in skin moisture, which may contribute to loss of firmness. Clinical trials have shown that topically applied ROL effectively reduces wrinkles and helps retain youthful appearance. In the current study, ROL was shown to induce HA production and stimulates the gene expression of all three forms of hyaluronic acid synthases (HAS) in normal human epidermal keratinocytes monolayer cultures. Moreover, in human skin equivalent tissues and in human skin explants, topical treatment of tissues with a stabilized-ROL formulation significantly induced the gene expression of HAS mRNA concomitant with an increased HA production. Finally, in a vehicle-controlled human clinical study, histochemical analysis confirmed increased HA accumulation in the epidermis in ROL-treated human skin as compared to vehicle. These results show that ROL increases skin expression of HA, a significant contributing factor responsible for wrinkle formation and skin moisture, which decrease during aging. Taken together with the activity to increase collagen, elastin, and cell proliferation, these studies establish that retinol provides multi-functional activity for photodamaged skin.

A multi-scaled hybrid orthopedic implant: bone ECM-shaped Sr-HA nanofibers on the microporous walls of a macroporous titanium scaffold.

Science.gov (United States)

Han, Yong; Zhou, Jianhong; Zhang, Lan; Xu, Kewei

2011-07-08

We report here, for the first time, a novel multi-scaled hybrid orthopedic implant material consisting of a macroporous Ti scaffold, whose macropores' walls have a microporous titania layer which is fully covered with nanofibers of Sr-doped hydroxyapatite (Sr-HA). The microporous titania layer is formed on and within the Ti scaffold by micro-arc oxidation, which firmly binds to the Ti substrate and contains Ca2+, Sr2+ and PO4(3-) ions. It is then hydrothermally treated to form Sr-HA nanofibers. During the hydrothermal treatment, Sr-HA nanoprisms nucleate from Ca0.5Sr0.5TiO3 pre-formed on the TiO2 and grow in length to nanofibers at the expense of Ca2+, Sr2+ and PO4(3-) ions that migrate from the TiO2. These Sr-HA nanofibers construct a network structure similar to the hierarchical organization of bone extracellular matrix (ECM), and the resulting nanofibrous surface displays a firm adhesion to substrate, superhydrophilicity and apatite-inducing ability. The induced apatite prefers to nucleate on the basal-faceted surfaces of Sr-HA nanofibers. The nanofiber-walled scaffold has a great potential for load-bearing orthotopic use.

Plan for the Startup of HA-21I Furnace Operations at the Plutonium Finishing Plant (PFP)

International Nuclear Information System (INIS)

WILLIS, H.T.

2000-01-01

Achievement of Thermal Stabilization mission elements require the installation and startup of three additional muffle furnaces for the thermal stabilization of plutonium and plutonium bearing materials at the Plutonium Finishing Plant (PFP). The release to operate these additional furnaces will require an Activity Based Startup Review. The conduct of the Activity Based Startup Review (ABSR) was approved by Fluor Daniel Hanford on October 15, 1999. This plan has been developed with the objective of identifying those activities needed to guide the controlled startup of five furnaces from authorization to unrestricted operations by adding the HA-211 furnaces in an orderly and safe manner after the approval to Startup has been given. The Startup Plan provides a phased approach that bridges the activities between the completion of the Activity Based Startup Review authorizing the use of the three additional furnaces and the unrestricted operation of the five thermal stabilization muffle furnaces. The four phases are: (1) the initiation of five furnace operations using three empty (simulated full) boat charges from HA-211 and two full charges from HC-21C; (2) three furnace operations (one full charge from HA-211 and two full charges from HC-21C); (3) four furnace operations (two full charges from HA-211 and two full charges from HC-21C); and (4) integrated five furnace operations and unrestricted operations. Phase 1 of the Plan will be considered as the cold runs. This Plan also provides management oversight and administrative controls that are to be implemented until unrestricted operations are authorized. It also provides a formal review process for ensuring that all preparations needed for full five furnace operations are completed and formally reviewed prior to proceeding to the increased activity levels associated with five furnace operations. Specific objectives include: (1) To ensure that activities are conducted in a safe manner. (2) To provide supplemental

MiR-30e suppresses proliferation of hepatoma cells via targeting prolyl 4-hydroxylase subunit alpha-1 (P4HA1) mRNA

Energy Technology Data Exchange (ETDEWEB)

Feng, Guoxing [State Key Laboratory of Medicinal Chemical Biology, Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin (China); Shi, Hui [State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin (China); Li, Jiong; Yang, Zhe [State Key Laboratory of Medicinal Chemical Biology, Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin (China); Fang, Runping; Ye, Lihong [State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin (China); Zhang, Weiying, E-mail: zhwybao@nankai.edu.cn [State Key Laboratory of Medicinal Chemical Biology, Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin (China); Zhang, Xiaodong, E-mail: zhangxd@nankai.edu.cn [State Key Laboratory of Medicinal Chemical Biology, Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin (China)

2016-04-08

Aberrant microRNA expression has been shown to be characteristic of many cancers. It has been reported that the expression levels of miR-30e are decreased in liver cancer tissues. However, the role of miR-30e in hepatocellular carcinoma remains poorly understood. In the present study, we investigated the significance of miR-30e in hepatocarcinogenesis. Bioinformatics analysis reveals a putative target site of miR-30e in the 3′-untranslated region (3′UTR) of prolyl 4-hydroxylase subunit alpha-1 (P4HA1) mRNA. Moreover, luciferase reporter gene assays verified that miR-30e directly targeted 3′UTR of P4HA1 mRNA. Then, we demonstrated that miR-30e was able to reduce the expression of P4HA1 at the levels of mRNA and protein using reverse transcription-polymerase chain reaction and Western blot analysis. Enforced expression of miR-30e suppressed proliferation of HepG2 cells by 5-ethynyl-2-deoxyuridine (EdU) assay and reduced colony formation of these cells by colony formation analysis. Conversely, anti-miR-30e enhanced the proliferation of hepatoma cells in vitro. Interestingly, the ectopic expression of P4HA1 could efficiently rescue the inhibition of cell proliferation mediated by miR-30e in HepG2 cells. Meanwhile, silencing of P4HA1 abolished the anti-miR-30e-induced proliferation of cells. Clinically, quantitative real-time PCR showed that miR-30e was down-regulated in liver tumor tissues relative to their peritumor tissues. The expression levels of miR-30e were negatively correlated to those of P4HA1 mRNA in clinical liver tumor tissues. Thus, we conclude that miR-30e suppresses proliferation of hepatoma cells through targeting P4HA1 mRNA. Our finding provides new insights into the mechanism of hepatocarcinogenesis. - Highlights: • P4HA1 is a novel target gene of miR-30e. • P4HA1 is increased in clinical HCC tissues. • MiR-30e is negatively correlated with P4HA1 in clinical HCC tissues. • MiR-30e suppresses the proliferation of HCC cells through

Investigation of the HA film deposited on the porous Ti6Al4V alloy prepared via additive manufacturing

International Nuclear Information System (INIS)

Surmeneva, M; Chudinova, E; Syrtanov, M; Surmenev, R; Koptioug, A

2015-01-01

This study is focused on the use of radio frequency magnetron sputtering to modify the surface of porous Ti6Al4V alloy fabricated via additive manufacturing technology. The hydroxyapatite (HA) coated porous Ti6Al4V alloy was studied in respect with its chemical and phase composition, surface morphology, water contact angle and hysteresis, and surface free energy. Thin nanocrystalline HA film was deposited while its structure with diamond-shaped cells remained unchanged. Hysteresis and water contact angle measurements revealed an effect of the deposited HA films, namely an increased water contact angle and contact angle hysteresis. The increase of the contact angle of the coating-substrate system compared to the uncoated substrate was attributed to the multiscale structure of the resulted surfaces. (paper)

AFLP and AMP Fingerprints as Markers to Evaluate Genetic Differences between Medicago truncatula Line Jemalong and 2HA, a New Line Produced by in vitro Culture Selection

Directory of Open Access Journals (Sweden)

R.R. Irwanto

2008-09-01

Full Text Available A new line, Medicago truncatula cv. Jemalong 2HA (herein known as 2HA has been developed via repetitive regeneration and selection of M. truncatula cv. Jemalong. During somatic embryogenesis, 2HA produces 500 times more embryos than its progenitor, Jemalong. It is interesting to study if those two lines are isogenic or has genetic differences. The main objectives of the study was to evaluate the genotypic differences between Jemalong and 2HA also to evaluate the methylation event in 2HA utilized two DNA fingerprinting techniques, i.e AFLP fingerprints (Amplified Length of Polymorphism and AMP (Amplified Methylation Polymorphism. The results showed that AFLP analysis using eight primers combinations could not detect any differences between Jemalong and 2HA. However, using AMP methylation sensitive primers it could detect 15 polymorphisms out of 840 markers. These results lead to a conclusion that Jemalong and 2HA are isogenic lines. 2HA may have higher regeneration capacities due to methylation process which occurs during the production of 2HA through repetitive regeneration cycles.

Amino Acid Substitutions Improve the Immunogenicity of H7N7HA Protein and Protect Mice against Lethal H7N7 Viral Challenge.

Directory of Open Access Journals (Sweden)

Subaschandrabose Rajesh Kumar

Full Text Available Avian influenza A H7N7/NL/219/03 virus creates a serious pandemic threat to human health because it can transmit directly from domestic poultry to humans and from human to human. Our previous vaccine study reported that mice when immunized intranasally (i.n with live Bac-HA were protected from lethal H7N7/NL/219/03 challenge, whereas incomplete protection was obtained when administered subcutaneously (s.c due to the fact that H7N7 is a poor inducer of neutralizing antibodies. Interestingly, our recent vaccine studies reported that mice when vaccinated subcutaneously with Bac-HA (H7N9 was protected against both H7N9 (A/Sh2/2013 and H7N7 virus challenge. HA1 region of both H7N7 and H7N9 viruses are differ at 15 amino acid positions. Among those, we selected three amino acid positions (T143, T198 and I211 in HA1 region of H7N7. These amino acids are located within or near the receptor binding site. Following the selection, we substituted the amino acid at these three positions with amino acids found on H7N9HA wild-type. In this study, we evaluate the impact of amino acid substitutions in the H7N7 HA-protein on the immunogenicity. We generated six mutant constructs from wild-type influenza H7N7HA cDNA by site directed mutagenesis, and individually expressed mutant HA protein on the surface of baculovirus (Bac-HAm and compared their protective efficacy of the vaccines with Bac-H7N7HA wild-type (Bac-HA by lethal H7N7 viral challenge in a mouse model. We found that mice immunized subcutaneously with Bac-HAm constructs T143A or T198A-I211V or I211V-T143A serum showed significantly higher hemagglutination inhibition and neutralization titer against H7N7 and H7N9 viruses when compared to Bac-HA vaccinated mice groups. We also observed low level of lung viral titer, negligible weight loss and complete protection against lethal H7N7 viral challenge. Our results indicated that amino acid substitution at position 143 or 211 improve immunogenicity of H7N7HA

In vitro and in vivo performance of bioactive Ti6Al4V/TiC/HA implants fabricated by a rapid microwave sintering technique

International Nuclear Information System (INIS)

Choy, Man Tik; Tang, Chak Yin; Chen, Ling; Wong, Chi Tak; Tsui, Chi Pong

2014-01-01

Failure of the bone–implant interface in a joint prosthesis is a main cause of implant loosening. The introduction of a bioactive substance, hydroxyapatite (HA), to a metallic bone–implant may enhance its fixation on human bone by encouraging direct bone bonding. Ti6Al4V/TiC/HA composites with a reproducible porous structure (porosity of 27% and pore size of 6–89 μm) were successfully fabricated by a rapid microwave sintering technique. This method allows the biocomposites to be fabricated in a short period of time under ambient conditions. Ti6Al4V/TiC/HA composites exhibited a compressive strength of 93 MPa, compressive modulus of 2.9 GPa and microhardness of 556 HV which are close to those of the human cortical bone. The in vitro preosteoblast MC3T3-E1 cells cultured on the Ti6Al4V/TiC/HA composite showed that the composite surface could provide a biocompatible environment for cell adhesion, proliferation and differentiation without any cytotoxic effects. This is among the first attempts to study the in vivo performance of load-bearing Ti6Al4V/TiC and Ti6Al4V/TiC/HA composites in a live rabbit. The results indicated that the Ti6Al4V/TiC/HA composite had a better bone–implant interface compared with the Ti6Al4V/TiC implant. Based on the microstructural features, the mechanical properties, and the in vitro and in vivo test results from this study, the Ti6Al4V/TiC/HA composites have the potential to be employed in load-bearing orthopedic applications. - Highlights: • Ti6Al4V/TiC and Ti6Al4V/TiC/HA composites were fabricated by microwave sintering. • Ti6Al4V/TiC/HA exhibited mechanical properties close to human cortical bone. • Ti6Al4V/TiC/HA could provide a biocompatible environment for bone cell growth. • Ti6Al4V/TiC/HA showed a better bone–implant interface than Ti6Al4V/TiC. • Ti6Al4V/TiC/HA could be used for bone replacement under load-bearing conditions

Comparative histomorphometric analysis between α-Tcp cement and β-Tcp/Ha granules in the bone repair of rat calvaria

Directory of Open Access Journals (Sweden)

Gisela Grandi

2011-03-01

Full Text Available This study compared the effect of two bioceramics on the process of bone repair: α-tricalcium phosphate (α-TCP cement and β-tricalcium phosphate hydroxyapatite particles (β-TCP/HA. Calvarial defects were created in 50 rats, divided into two groups (α and β/HA. Software was used at 7, 21, 60, 90 and 120 days to assess bone formation. Mean new bone formation rates were as follows: α group, 1.6% at 7 days, 5.24% at 21 days, 24% at 60 days, 30.21% at 90 days and 50.59% at 120 days; β/HA group, 1.94% at 7 days, 2.53% at 21 days, 12.47% at 60 days, 26.84% at 90 days and 38.82% at 120 days; control group, 0.15% at 7 days, 10.12% at 21 days, 15.10% at 60 days, 18.94% at 90 days, 48.50% at 120 days. Both materials are osteoconductive and biocompatible. Perhaps the larger rate of new bone formation observed in the α-TCP group, it also occurs in the β-TCP/HA group within a longer time period.

Increase in the fraction of necrotic, not apoptotic, cells in SiHa xenograft tumours shortly after irradiation

International Nuclear Information System (INIS)

Olive, P.L.; Vikse, C.M.; Vanderbyl, S.

1999-01-01

Background and purpose: Approximately 18% of the cells recovered by rapid mechanical dissociation of SiHa xenograft tumours contain large numbers of DNA strand breaks. The number of damaged cells increases to 30-40% 4-6 h after exposure to 5 or 15 Gy, returning to normal levels by 12 h. This observation is reminiscent of the rate of production of apoptotic cells in other murine and human xenograft tumours. The nature of this damage, rate of development and relation to cell proliferation rate were therefore examined in detail.Materials and methods: SiHa human cervical carcinoma cells were grown as xenograft tumours in SCID mice. Single-cell suspensions were prepared as a function of time after irradiation of the mouse and examined for DNA damage using the alkaline comet assay. Cell cycle progression was measured by flow cytometry evaluation of anti-bromodeoxyuridine-labelled tumour cells.Results: Significant numbers of apoptotic cells could not be detected in irradiated SiHa tumours using an end-labelling assay, electron microscopy, or histological examination of thin sections. Instead, xenograft cells exhibiting extensive DNA damage in the comet assay were predominantly necrotic cells. The increase in the proportion of heavily damaged cells 4-6 h after irradiation could be the result of an interplay between several factors including loss of viable cells and change in production or loss of necrotic cells. Analysis of the progression of BrdUrd-labelled cells confirmed that while 35% of cells from untreated SiHa tumours had divided and entered G 1 phase by 6 h after BrdUrd injection, none of the labelled cells from tumours exposed to 5 or 15 Gy had progressed to G 1 .Conclusions: The increase in the percentage of SiHa tumour cells with extensive DNA damage 4-6 h after irradiation is attributable to necrosis, not apoptosis. Cell cycle progression and cell loss are likely to influence the kinetics of appearance of both apoptotic and necrotic cells in irradiated tumours

Generation of Oxtr cDNA(HA)-Ires-Cre Mice for Gene Expression in an Oxytocin Receptor Specific Manner.

Science.gov (United States)

Hidema, Shizu; Fukuda, Tomokazu; Hiraoka, Yuichi; Mizukami, Hiroaki; Hayashi, Ryotaro; Otsuka, Ayano; Suzuki, Shingo; Miyazaki, Shinji; Nishimori, Katsuhiko

2016-05-01

The neurohypophysial hormone oxytocin (OXT) and its receptor (OXTR) have critical roles in the regulation of pro-social behaviors, including social recognition, pair bonding, parental behavior, and stress-related responses. Supporting this hypothesis, a portion of patients suffering from autism spectrum disorder have mutations, such as single nucleotide polymorphisms, or epigenetic modifications in their OXTR gene. We previously reported that OXTR-deficient mice exhibit pervasive social deficits, indicating the critical role of OXTR in social behaviors. In the present study, we generated Oxtr cDNA(HA)-Ires-Cre knock-in mice, expressing both OXTR and Cre recombinase under the control of the endogenous Oxtr promoter. Knock-in cassette of Oxtr cDNA(HA)-Ires-Cre consisted of Oxtr cDNA tagged with the hemagglutinin epitope at the 3' end (Oxtr cDNA(HA)), internal ribosomal entry site (Ires), and Cre. Cre was expressed in the uterus, mammary gland, kidney, and brain of Oxtr cDNA(HA)-Ires-Cre knock-in mice. Furthermore, the distribution of Cre in the brain was similar to that observed in Oxtr-Venus fluorescent protein expressing mice (Oxtr-Venus), another animal model previously generated by our group. Social behavior of Oxtr cDNA(HA)-Ires-Cre knock-in mice was similar to that of wild-type animals. We demonstrated that this construct is expressed in OXTR-expressing neurons specifically after an infection with the recombinant adeno-associated virus carrying the flip-excision switch vector. Using this system, we showed the transport of the wheat-germ agglutinin tracing molecule from the OXTR-expressing neurons to the innervated neurons in knock-in mice. This study might contribute to the monosynaptic analysis of neuronal circuits and to the optogenetic analysis of neurons expressing OXTR. © 2015 Wiley Periodicals, Inc.

Titanium Dioxide Nanoparticle Penetration into the Skin and Effects on HaCaT Cells.

Science.gov (United States)

Crosera, Matteo; Prodi, Andrea; Mauro, Marcella; Pelin, Marco; Florio, Chiara; Bellomo, Francesca; Adami, Gianpiero; Apostoli, Pietro; De Palma, Giuseppe; Bovenzi, Massimo; Campanini, Marco; Filon, Francesca Larese

2015-08-07

Titanium dioxide nanoparticles (TiO2NPs) suspensions (concentration 1.0 g/L) in synthetic sweat solution were applied on Franz cells for 24 h using intact and needle-abraded human skin. Titanium content into skin and receiving phases was determined. Cytotoxicity (MTT, AlamarBlue(®) and propidium iodide, PI, uptake assays) was evaluated on HaCat keratinocytes after 24 h, 48 h, and seven days of exposure. After 24 h of exposure, no titanium was detectable in receiving solutions for both intact and damaged skin. Titanium was found in the epidermal layer after 24 h of exposure (0.47 ± 0.33 μg/cm(2)) while in the dermal layer, the concentration was below the limit of detection. Damaged skin, in its whole, has shown a similar concentration (0.53 ± 0.26 μg/cm(2)). Cytotoxicity studies on HaCaT cells demonstrated that TiO2NPs induced cytotoxic effects only at very high concentrations, reducing cell viability after seven days of exposure with EC50s of 8.8 × 10(-4) M (MTT assay), 3.8 × 10(-5) M (AlamarBlue(®) assay), and 7.6 × 10(-4) M (PI uptake, index of a necrotic cell death). Our study demonstrated that TiO2NPs cannot permeate intact and damaged skin and can be found only in the stratum corneum and epidermis. Moreover, the low cytotoxic effect observed on human HaCaT keratinocytes suggests that these nano-compounds have a potential toxic effect at the skin level only after long-term exposure.

A complete analysis of HA and NA genes of influenza A viruses.

LENUS (Irish Health Repository)

Shi, Weifeng

2010-12-01

More and more nucleotide sequences of type A influenza virus are available in public databases. Although these sequences have been the focus of many molecular epidemiological and phylogenetic analyses, most studies only deal with a few representative sequences. In this paper, we present a complete analysis of all Haemagglutinin (HA) and Neuraminidase (NA) gene sequences available to allow large scale analyses of the evolution and epidemiology of type A influenza.

β-TCP/HA with or without enamel matrix proteins for maxillary sinus floor augmentation

DEFF Research Database (Denmark)

Nery, James Carlos; Pereira, Luís Antônio Violin Dias; Guimarães, George Furtado

2017-01-01

BACKGROUND: It is still unclear whether enamel matrix proteins (EMD) as adjunct to bone grafting enhance bone healing. This study compared histomorphometrically maxillary sinus floor augmentation (MSFA) with β-TCP/HA in combination with or without EMD in humans. METHODS: In ten systemically healthy...

ADCC employing an NK cell line (haNK) expressing the high affinity CD16 allele with avelumab, an anti-PD-L1 antibody.

Science.gov (United States)

Jochems, Caroline; Hodge, James W; Fantini, Massimo; Tsang, Kwong Y; Vandeveer, Amanda J; Gulley, James L; Schlom, Jeffrey

2017-08-01

NK-92 cells, and their derivative, designated aNK, were obtained from a patient with non-Hodgkin lymphoma. Prior clinical studies employing adoptively transferred irradiated aNK cells have provided evidence of clinical benefit and an acceptable safety profile. aNK cells have now been engineered to express IL-2 and the high affinity (ha) CD16 allele (designated haNK). Avelumab is a human IgG1 anti-PD-L1 monoclonal antibody, which has shown evidence of clinical activity in a range of human tumors. Prior in vitro studies have shown that avelumab has the ability to mediate antibody-dependent cell-mediated cytotoxicity (ADCC) of human tumor cells when combined with NK cells. In the studies reported here, the ability of avelumab to enhance the lysis of a range of human carcinoma cells by irradiated haNK cells via the ADCC mechanism is demonstrated; this ADCC is shown to be inhibited by anti-CD16 blocking antibody and by concanamycin A, indicating the use of the granzyme/perforin pathway in tumor cell lysis. Studies also show that while NK cells have the ability to lyse aNK or haNK cells, the addition of NK cells to irradiated haNK cells does not inhibit haNK-mediated lysis of human tumor cells, with or without the addition of avelumab. Avelumab-mediated lysis of tumor cells by irradiated haNK cells is also shown to be similar to that of NK cells bearing the V/V Fc receptor high affinity allele. These studies thus provide the rationale for the clinical evaluation of the combined use of avelumab with that of irradiated adoptively transferred haNK cells. © 2017 UICC.

Study on effect of artemisinin combined with 60Co γ-ray on DNA damage in HeLa and SiHa cells

International Nuclear Information System (INIS)

Feng Yang; Zhou Yuanyuan; Yang Wei; Chen Qiu; Li Ming; Zhang Shuyu; Zhu Wei; Cao Jianping; Zhang Xuguang

2011-01-01

Objective: To investigate the effect of Artemisinin combined with 60 Co γ-ray on DNA damage in HeLa and SiHa cells of human cervical cancer. Methods: Cell growth kinetics was evaluated by MTT assay to determine the most appropriate drug concentration. Effects of Artemisinin combined with 60 Co γ-ray on DNA damage in HeLa and SiHa cells were detected by single cell gel electrophoresis. Results: With the concentration increased during the effect of Artemisinin, the HeLa and SiHa cells had higher inhibition on cell proliferation. The SCGE showed that:the comet cell analysis indexes (the comet cells ratio, Tail Length, Olive Tail Moment and Tail DNA%) there was no statistic difference in between the artemisinin group and the control group (P>0.05). With radiation in the same dose, the comet cell analysis indexes of Hela cells treated with both artermisinin and exposed to radiation were higher than that only exposed to radiation group(P 0.05). Conclusion: Artemisinin can not induce DNA damage in both HeLa and SiHa cells, but it can make irradiated HeLa cells DNA damage to be aggravated and enhance HeLa cells' radiation sensitivity. However, Artemisinin has no radiosensitizing effect on SiHa cells. (authors)

A multi-scaled hybrid orthopedic implant: bone ECM-shaped Sr-HA nanofibers on the microporous walls of a macroporous titanium scaffold

Energy Technology Data Exchange (ETDEWEB)

Han Yong; Zhou Jianhong; Zhang Lan; Xu Kewei, E-mail: yonghan@mail.xjtu.edu.cn [State Key Laboratory for Mechanical Behavior of Materials, Xi' an Jiaotong University, Xi' an 710049 (China)

2011-07-08

We report here, for the first time, a novel multi-scaled hybrid orthopedic implant material consisting of a macroporous Ti scaffold, whose macropores' walls have a microporous titania layer which is fully covered with nanofibers of Sr-doped hydroxyapatite (Sr-HA). The microporous titania layer is formed on and within the Ti scaffold by micro-arc oxidation, which firmly binds to the Ti substrate and contains Ca{sup 2+}, Sr{sup 2+} and PO{sub 4}{sup 3-} ions. It is then hydrothermally treated to form Sr-HA nanofibers. During the hydrothermal treatment, Sr-HA nanoprisms nucleate from Ca{sub 0.5}Sr{sub 0.5}TiO{sub 3} pre-formed on the TiO{sub 2} and grow in length to nanofibers at the expense of Ca{sup 2+}, Sr{sup 2+} and PO{sub 4}{sup 3-} ions that migrate from the TiO{sub 2}. These Sr-HA nanofibers construct a network structure similar to the hierarchical organization of bone extracellular matrix (ECM), and the resulting nanofibrous surface displays a firm adhesion to substrate, superhydrophilicity and apatite-inducing ability. The induced apatite prefers to nucleate on the basal-faceted surfaces of Sr-HA nanofibers. The nanofiber-walled scaffold has a great potential for load-bearing orthotopic use.

(+)-Nootkatone inhibits tumor necrosis factor α/interferon γ-induced production of chemokines in HaCaT cells.

Science.gov (United States)

Choi, Hyeon-Jae; Lee, Jin-Hwee; Jung, Yi-Sook

2014-05-02

Chemokines are important mediators of cell migration, and thymus and activation-regulated chemokine (TARC/CCL17) and macrophage-derived chemokine (MDC/CCL22) are well-known typical inflammatory chemokines involved in atopic dermatitis (AD). (+)-Nootkatone is the major component of Cyperus rotundus. (+)-Nootkatone has antiallergic, anti-inflammatory, and antiplatelet activities. The purpose of this study was to investigate the effect of (+)-nootkatone on tumor necrosis factor α (TNF-α)/interferon γ (IFN-γ)-induced expression of Th2 chemokines in HaCaT cells. We found that (+)-nootkatone inhibited the TNF-α/IFN-γ-induced expression of TARC/CCL17 and MDC/CCL22 mRNA in HaCaT cells. It also significantly inhibited TNF-α/IFN-γ-induced activation of nuclear factor kappa B (NF-κB), p38 mitogen-activated protein kinase (MAPK), and protein kinase Cζ (PKCζ). Furthermore, we showed that PKCζ and p38 MAPK contributed to the inhibition of TNF-α/IFN-γ-induced TARC/CCL17 and MDC/CCL22 expression by blocking IκBα degradation in HaCaT cells. Taken together, these results suggest that (+)-nootkatone may suppress TNF-α/IFN-γ-induced TARC/CCL17 and MDC/CCL22 expression in HaCaT cells by inhibiting of PKCζ and p38 MAPK signaling pathways that lead to activation of NF-κB. We propose that (+)-nootkatone may be a useful therapeutic candidate for inflammatory skin diseases such as AD. Copyright © 2014 Elsevier Inc. All rights reserved.

Formulation Changes Affect Material Properties and Cell Behavior in HA-Based Hydrogels

Directory of Open Access Journals (Sweden)

Thomas Lawyer

2012-01-01

Full Text Available To develop and optimize new scaffold materials for tissue engineering applications, it is important to understand how changes to the scaffold affect the cells that will interact with that scaffold. In this study, we used a hyaluronic acid- (HA- based hydrogel as a synthetic extracellular matrix, containing modified HA (CMHA-S, modified gelatin (Gtn-S, and a crosslinker (PEGda. By varying the concentrations of these components, we were able to change the gelation time, enzymatic degradation, and compressive modulus of the hydrogel. These changes also affected fibroblast spreading within the hydrogels and differentially affected the proliferation and metabolic activity of fibroblasts and mesenchymal stem cells (MSCs. In particular, PEGda concentration had the greatest influence on gelation time, compressive modulus, and cell spreading. MSCs appeared to require a longer period of adjustment to the new microenvironment of the hydrogels than fibroblasts. Fibroblasts were able to proliferate in all formulations over the course of two weeks, but MSCs did not. Metabolic activity changed for each cell type during the two weeks depending on the formulation. These results highlight the importance of determining the effect of matrix composition changes on a particular cell type of interest in order to optimize the formulation for a given application.

The Sanskrit and Arabic Sources of the Praśnatantra Attributed to Nīlakaṇṭha

Directory of Open Access Journals (Sweden)

Martin Gansten

2014-12-01

Full Text Available The highly popular Praśnatantra attributed to Nīlakaṇṭha of Kāśī (fl. late 16th century and sometimes regarded as the third volume of his Tājikanīlakaṇṭhī is shown to depend for its basic structure on an abridged Sanskrit version of the Kitāb fi l-masāʾil wa-l-aḥkām by Sahl ibn Bishr (early 9th century, apparently authored by Samarasiṃha in the 13th century, to which quotations primarily from Sanskrit astrological works in the classical Indian style have been added, resulting in a hybrid of Indian and Perso-Arabic interrogational astrology.

The mechanism of kaempferol induced apoptosis and inhibited proliferation in human cervical cancer SiHa cell: From macro to nano.

Science.gov (United States)

Tu, Lv-Ying; Bai, Hai-Hua; Cai, Ji-Ye; Deng, Sui-Ping

2016-11-01

Kaempferol has been identified as a potential cancer therapeutic agent by an increasing amount of evidences. However, the changes in the topography of cell membrane induced by kaempferol at subcellular- or nanometer-level were still unclear. In this work, the topographical changes of cytomembrane in human cervical cancer cell (SiHa) induced by kaempferol, as well as the role of kaempferol in apoptosis induction and its possible mechanisms, were investigated. At the macro level, MTT assays showed that kaempferol inhibited the proliferation of SiHa cells in a time- and dose-dependent manner. Flow cytometry analysis demonstrated that kaempferol could induce SiHa cell apoptosis, mitochondrial membrane potential disruption, and intracellular free calcium elevation. At the micro level, fluorescence imaging by laser scanning confocal microscopy (LSCM) indicated that kaempferol could also destroy the networks of microtubules. Using high resolution atomic force microscopy (AFM), we determined the precise changes of cellular membrane induced by kaempferol at subcellular or nanometer level. The spindle-shaped SiHa cells shrank after kaempferol treatment, with significantly increased cell surface roughness. These data showed structural characterizations of cellular topography in kaempferol-induced SiHa cell apoptosis and might provide novel integrated information from macro to nano level to assess the impact of kaempferol on cancer cells, which might be important for the understanding of the anti-cancer mechanisms of drugs. SCANNING 38:644-653, 2016. © 2016 Wiley Periodicals, Inc. © Wiley Periodicals, Inc.

HA03 as an Iranian Candidate Concealed Antigen for Vaccination against Hyalomma anatolicum anatolicum: Comparative Structural and In silico Studies

Directory of Open Access Journals (Sweden)

Mohammadi, A.

2013-12-01

Full Text Available In the last decades researchers had focused on developing a vaccine against tick based on protective antigen. Recombinant vaccines based on concealed antigen from Boophilus microplus have been developed in Australia and Cuba by the name of TICKGARD and GAVAC (De La Fuente and Kocan, 2006. Further studies on this antigen have shown some extent of protection against other species (De Vos et al., 2001. In Iran most important species is Hyalomma anatolicum and limited information about its control are available. This paper reports structural and polymorphic analysis of HA03 as an Iranian candidate concealed antigen of H. a. anatolicum deposited in Gen-Bank .(Aghaeipour et al. GQ228820. The comparison between this antigen and other mid gut concealed antigen that their characteristics are available in GenBank showed there are high rate of similarity between them. The HA03 amino acid sequence had a homology of around 89%, 64%, 56% with HA98, BM86, BM95 respectively. Potential of MHC class I and II binding region indicated a considerable variation between BM86 antigen and its efficiency against Iranian H. a. anatolicum. In addition, predicted major of hydrophobisity and similarity in N-glycosylation besides large amount of cystein and seven EGF like regions presented in protein structure revealed that value of HA03 as a new protective antigen and the necessity of the development, BM86 homolog of H. a. anatolicum HA03 based recombinant vaccine.

Análisis de clusters en Andalucía

Directory of Open Access Journals (Sweden)

Antonio Antúnez Torres

2008-06-01

Full Text Available El análisis de clusters ha llegado a ser un conocido instrumento en la determinación de las innovaciones y el poder competitivo de las economías nacionales y regionales, siendo amplia la variedad de métodos que los investigadores han aplicado en la identificación de los clusters industriales claves. Este trabajo presenta los resultados obtenidos con diferentes metodologías en la identificación de los clusters económicos agregados de Andalucía, “mega-clusters”, utilizando los últimos datos input output disponibles (año 2000. El primer método, basado en la teoría de grafos, clasifica los sectores de acuerdo a la importancia de su mutua dependencia por sus envíos y compras. El segundo, basado en la aplicación de un análisis de componentes principales a la tabla input-output, proporciona clusters conforme a la similitud de las estructuras de compras/ventas intermedias.

El habitat rural indígena en la provincia de Cáceres: problemática de su estudio

Directory of Open Access Journals (Sweden)

José Antonio REDONDO RODRÍGUEZ

2010-02-01

Full Text Available RESUMEN: Antes de abordar la problemática del habitat rural indígena cacereño, queremos dejar constancia de que las conclusiones a las que hemos llegado, son fruto de las excavaciones y prospecciones efectuadas en la década de los 80 por los miembros del Area de Historia Antigua del Departamento de Ciencias de la Antigüedad de la Universidad de Extremadura. Entre éstas convendría destacar las excavaciones llevadas a cabo en La Coraja (Aldeacentenera, El Castillejo (Santiago del Campo, La Villavieja de la Orden (Alcántara; Villasviejas del Tamuja (Botija y en Sansueña (Arroyo de la Luz; igualmente ha sido de suma importancia, sobre todo para consideraciones de tipo general, el proyecto de investigación «Los castros prerromanos de la provincia de Cáceres: planimetría y descripción» que aún está en proceso de elaboración.

Análisis de clusters en Andalucía

Directory of Open Access Journals (Sweden)

Antonio Antúnez Torres

2008-01-01

Full Text Available El análisis de clusters ha llegado a ser un conocido instrumento en la determinación de las innovaciones y el poder competitivo de las economías nacionales y regionales, siendo amplia la variedad de métodos que los investigadores han aplicado en la identificación de los clusters industriales claves. Este trabajo presenta los resultados obtenidos con diferentes metodologías en la identificación de los clusters económicos agregados de Andalucía, "mega-clusters", utilizando los últimos datos input output disponibles (año 2000. El primer método, basado en la teoría de grafos, clasifica los sectores de acuerdo a la importancia de su mutua dependencia por sus envíos y compras. El segundo, basado en la aplicación de un análisis de componentes principales a la tabla input-output, proporciona clusters conforme a la similitud de las estructuras de compras/ventas intermedias.

Relaciones sexuales y rendimiento físico: esclareciendo el mito del efecto perjudicial de mantener relaciones sexuales coitales antes de participar en actividades físicas extenuantes

Directory of Open Access Journals (Sweden)

M. Sc. José Moncada Jiménez

2006-03-01

Full Text Available La presente revisión bibliográfica se enfoca en presentar la evidencia científica que contradice el mito de que mantener relaciones sexuales coitales es perjudicial antes de realizar alguna actividad que requiere de un rendimiento físico máximo. Aunque en la actualidad este mito sigue difundiéndose en algunos ambientes deportivos, no existen estudios que indiquen que mantener relaciones sexuales coitales sea perjudicial. Con base en mediciones de parámetros fisiológicos, metabólicos y psicológicos, se ha llegado a determinar que el coito no afecta negativa ni positivamente el rendimiento físico. La escasa evidencia científica hace que este tema cobre importancia y se sugiere que se realicen más investigaciones para brindar consejos y educación apropiada a las poblaciones físicamente activas de diversas edades, como es el caso de los deportistas.

Imágenes fotográficas como medio de relación entre ex-reclusos y sociedad. Una propuesta de aprendizaje-servicio para las artes.

Directory of Open Access Journals (Sweden)

Laia Moretó Alvarado

2014-11-01

Full Text Available Todavía no se ha llegado a un acuerdo sobre lo que caracteriza al aprendizaje-servicio en relación con otras metodologías. La idea de potenciar la reflexión del estudiante sobre el servicio que puede prestar a la sociedad es uno de los pilares fundamentales del ApS y encaja bien con la formación de los futuros profesionales. El presente artículo describe la experiencia de idear un proyecto de ApS utilizando la fotografía como medio de expresión del colectivo de expresos y de sensibilización de la sociedad. El proyecto fue planteado en el marco de una asignatura de formación básica del grado de Bellas Artes con el objetivo de introducir al estudiante en el contexto universitario de las artes, ayudarlo a vincular su discurso artístico con la propia tradición cultural e invitarle a intervenir en la sociedad como profesional.

Chemical and microstructural diversity of steel grades

Directory of Open Access Journals (Sweden)

Zorc, B.

2002-12-01

Full Text Available The aim of the paper is to show, using theoretical and practical analyses, chemical and microstructural differences among individual types of steel grades 355 found in the market. The mechanical properties required for these steels are achieved by alloying or thermomechanical treatment. It was found that the individual types of this steel are poorly weldable, particularly those of large thickness.

El objetivo del artículo es presentar, en base a un análisis teórico y práctico, las diferencias químicas y microestructurales entre los diferentes tipos de aceros calidad 355 que pueden encontrarse en el mercado. Las características mecánicas requeridas en estos aceros se consiguen con aleaciones, o bien a través de tratamientos termo-mecánicos.Se ha llegado a la conclusión de que determinados tipos de acero son más difíciles de soldar, en especial cuando se trata de espesores grandes.

miR-198 Represses the Proliferation of HaCaT Cells by Targeting Cyclin D2

Directory of Open Access Journals (Sweden)

Jian Wang

2015-07-01

Full Text Available Background: MiR-198 has been considered as an inhibitor of cell proliferation, invasion, migration and a promoter of apoptosis in most cancer cells, while its effect on non-cancer cells is poorly understood. Methods: The effect of miR-198 transfection on HaCaT cell proliferation was firstly detected using Cell Count Kit-8 and the cell cycle progression was analyzed by flow cytometry. Using bioinformatics analyses and luciferase assay, a new target of miR-198 was searched and identified. Then, the effect of the new target gene of miR-198 on cell proliferation and cell cycle was also detected. Results: Here we showed that miR-198 directly bound to the 3′-UTR of CCND2 mRNA, which was a key regulator in cell cycle progression. Overexpressed miR-198 repressed CCND2 expression at mRNA and protein levels and subsequently led to cell proliferation inhibition and cell cycle arrest in the G1 phase. Transfection ofSiCCND2 in HaCaT cells showed similar inhibitory effects on cell proliferation and cell cycle progression. Conclusion: In conclusion, we have identified that miR-198 inhibited HaCaT cell proliferation by directly targeting CCND2.

Metabolic activation of pyrrolizidine alkaloids leading to phototoxicity and photogenotoxicity in human HaCaT keratinocytes.

Science.gov (United States)

Wang, Chia-Chi; Xia, Qingsu; Li, Meng; Wang, Shuguang; Zhao, Yuewei; Tolleson, William H; Yin, Jun-Jie; Fu, Peter P

2014-01-01

Pyrrolizidine alkaloids, produced by a large number of poisonous plants with wide global distribution, are associated with genotoxicity, tumorigenicity, and hepatotoxicity in animals and humans. Mammalian metabolism converts pyrrolizidine alkaloids to reactive pyrrolic metabolites (dehydropyrrolizidine alkaloids) that form covalent protein and DNA adducts. Although a mechanistic understanding is currently unclear, pyrrolizidine alkaloids can cause secondary (hepatogenous) photosensitization and induce skin cancer. In this study, the phototoxicity of monocrotaline, riddelliine, dehydromonocrotaline, dehydroriddelliine, and dehydroretronecine (DHR) in human HaCaT keratinocytes under ultraviolet A (UVA) irradiation was determined. UVA irradiation of HaCaT cells treated with dehydromonocrotaline, dehydroriddelline, and DHR resulted in increased release of lactate dehydrogenase and enhanced photocytotoxicity proportional to the UVA doses. UVA-induced photochemical DNA damage also increased proportionally with dehydromonocrotaline and dehydroriddelline. UVA treatment potentiated the formation of 8-hydroxy-2'-deoxyguanosine DNA adducts induced by dehydromonocrotaline in HaCaT skin keratinocytes. Using electron spin resistance trapping, we found that UVA irradiation of dehydromonocrotaline and dehydroriddelliine generates reactive oxygen species (ROS), including hydroxyl radical, singlet oxygen, and superoxide, and electron transfer reactions, indicating that cytotoxicity and genotoxicity of these compounds could be mediated by ROS. Our results suggest that dehydropyrrolizidine alkaloids formed or delivered to the skin cause pyrrolizidine alkaloid-induced secondary photosensitization and possible skin cancer.

Haïti | CRDI - Centre de recherches pour le développement ...

International Development Research Centre (IDRC) Digital Library (Canada)

En dépit d'un climat et d'une topographie propices à la culture de fruits et de légumes, l'accès à des aliments sains et abordables constitue depuis longtemps un problème en Haïti. Grâce au financement accordé par le CRDI, des organismes locaux ont enseigné à 1 100 habitants de Port-au-Prince la culture vivrière ...

Effect of calcinations temperature on the luminescence intensity and fluorescent lifetime of Tb3+-doped hydroxyapatite (Tb-HA nanocrystallines

Directory of Open Access Journals (Sweden)

Hairong Yin

2017-06-01

Full Text Available Hydroxyapatite luminescent nanocrystallines doped with 6 mol.% Tb3+ (Tb-HA were prepared via chemical deposition method and calcined at different temperature, and the effects of calcinations temperature on the luminescence intensity and fluorescent lifetime were studied. TEM image of Tb-HA revealed that the shape of nanocrystallines changed from needle-like to short rod-like and sphere-like with the increase of calcinations temperature; while the particles sizes decreased from 190 nm to 110 nm. The crystallinity degree increased. The typical emission peaks attributed to Tb3+ ions were observed in emission spectra of 6 mol.% Tb-HA under 378 nm excitation. The luminescent intensity of Tb-HA, which showed the fluorescence quenching, firstly enhanced and then decreased at 700 °C; while the fluorescent lifetime increased firstly and then decreased after 600 °C. Furthermore, the ratio of intensity between 545 nm and 490 nm corresponding to electric-dipole and magnetic-dipole transition (IR: IO increases firstly and then decreases, which revealed that the proportion of substitute type and site of Ca2+ ions by Tb3+ ions were helpful to realize the substitute process and functional structure design.

A Novel HA/β-TCP-Collagen Composite Enhanced New Bone Formation for Dental Extraction Socket Preservation in Beagle Dogs

Directory of Open Access Journals (Sweden)

Ko-Ning Ho

2016-03-01

Full Text Available Past studies in humans have demonstrated horizontal and vertical bone loss after six months following tooth extraction. Many biomaterials have been developed to preserve bone volume after tooth extraction. Type I collagen serves as an excellent delivery system for growth factors and promotes angiogenesis. Calcium phosphate ceramics have also been investigated because their mineral chemistry resembles human bone. The aim of this study was to compare the performance of a novel bioresorbable purified fibrillar collagen and hydroxyapatite/β-tricalcium phosphate (HA/β-TCP ceramic composite versus collagen alone and a bovine xenograft-collagen composite in beagles. Collagen plugs, bovine graft-collagen composite and HA/β-TCP-collagen composite were implanted into the left and right first, second and third mandibular premolars, and the fourth molar was left empty for natural healing. In total, 20 male beagle dogs were used, and quantitative and histological analyses of the extraction ridge was done. The smallest width reduction was 19.09% ± 8.81% with the HA/β-TCP-collagen composite at Week 8, accompanied by new bone formation at Weeks 4 and 8. The HA/β-TCP-collagen composite performed well, as a new osteoconductive and biomimetic composite biomaterial, for socket bone preservation after tooth extraction.

Evaluation of a setting reaction pathway in the novel composite TiHA-CSD bone cement by FT-Raman and FT-IR spectroscopy

Science.gov (United States)

Paluszkiewicz, Czesława; Czechowska, Joanna; Ślósarczyk, Anna; Paszkiewicz, Zofia

2013-02-01

The aim of this study was to determine a setting reaction pathway in a novel, surgically handy implant material, based on calcium sulfate hemihydrate (CSH) and titanium doped hydroxyapatite (TiHA). The previous studies confirmed superior biological properties of TiHA in comparison to the undoped hydroxyapatite (HA) what makes it highly attractive for future medical applications. In this study the three types of titanium modified HA powders: untreated, calcined at 800 °C, sintered at 1250 °C and CSH were used to produce bone cements. The Fourier Transform-InfraRed (FT-IR) spectroscopy and Raman spectroscopy were applied to evaluate processes taking place during the setting of the studied materials. Our results undoubtedly confirmed that the reaction pathways and the phase compositions differed significantly for set cements and were dependent on the initial heat treatment of TiHA powder. Final materials were multiphase composites consisting of calcium sulfate dihydrate, bassanite, tricalcium phosphate, hydroxyapatite and calcium titanate (perovskite). The FT-IR and Scanning Electron Microscopy (SEM) measurements performed after the incubation of the cement samples in the simulated body fluid (SBF), indicate on high bioactive potential of the obtained bone cements.

Clinical significance of changes of serum levels of sICAM-1 and HA in patients with type-2 diabetes complicated with angiopathy

International Nuclear Information System (INIS)

Chen Lixia; Li Yugang

2004-01-01

Objective: To study the significance of changes of serum soluble intercellular adhesion molecule-1 (sICAM-1) and hyaluronic acid (HA) levels in patients with type-2 diabetes complicated with angiopathy. Methods: Serum levels of sICAM-1 (by ELISA) and HA (by RIA) were measured in 66 cases of type-2 diabetes complicated with angiopathy and 35 controls. Contents of fasting blood glucose (FBG), glycosylated hemoglobin (HbAIC) and urine microalbumin (MAlb) were also measured for correlation analysis studies. Results: Serum sICAM-1 and HA levels in patients with type-2 diabetic angiopathy were significantly higher than those in controls (p<0.01) and were closely positively correlated to the contents of FBG, HbAIC and MAlb (p<0.01). Conclusion: Higher serum sICAM-1 and HA levels may play some role in the pathogenesis of type-2 diabetic angiopathy

The performance of dental pulp stem cells on nanofibrous PCL/gelatin/nHA scaffolds.

NARCIS (Netherlands)

Yang, X.; Yang, F.; Walboomers, X.F.; Bian, Z.; Fan, M.; Jansen, J.A.

2010-01-01

The aim of current study is to investigate the in vitro and in vivo behavior of dental pulp stem cells (DPSCs) seeded on electrospun poly(epsilon-caprolactone) (PCL)/gelatin scaffolds with or without the addition of nano-hydroxyapatite (nHA). For the in vitro evaluation, DNA content, alkaline

Titanium Dioxide Nanoparticle Penetration into the Skin and Effects on HaCaT Cells

Directory of Open Access Journals (Sweden)

Matteo Crosera

2015-08-01

Full Text Available Titanium dioxide nanoparticles (TiO2NPs suspensions (concentration 1.0 g/L in synthetic sweat solution were applied on Franz cells for 24 h using intact and needle-abraded human skin. Titanium content into skin and receiving phases was determined. Cytotoxicity (MTT, AlamarBlue® and propidium iodide, PI, uptake assays was evaluated on HaCat keratinocytes after 24 h, 48 h, and seven days of exposure. After 24 h of exposure, no titanium was detectable in receiving solutions for both intact and damaged skin. Titanium was found in the epidermal layer after 24 h of exposure (0.47 ± 0.33 μg/cm2 while in the dermal layer, the concentration was below the limit of detection. Damaged skin, in its whole, has shown a similar concentration (0.53 ± 0.26 μg/cm2. Cytotoxicity studies on HaCaT cells demonstrated that TiO2NPs induced cytotoxic effects only at very high concentrations, reducing cell viability after seven days of exposure with EC50s of 8.8 × 10−4 M (MTT assay, 3.8 × 10−5 M (AlamarBlue® assay, and 7.6 × 10−4 M (PI uptake, index of a necrotic cell death. Our study demonstrated that TiO2NPs cannot permeate intact and damaged skin and can be found only in the stratum corneum and epidermis. Moreover, the low cytotoxic effect observed on human HaCaT keratinocytes suggests that these nano-compounds have a potential toxic effect at the skin level only after long-term exposure.

Induction of HIV neutralizing antibodies against the MPER of the HIV envelope protein by HA/gp41 chimeric protein-based DNA and VLP vaccines.

Directory of Open Access Journals (Sweden)

Ling Ye

Full Text Available Several conserved neutralizing epitopes have been identified in the HIV Env protein and among these, the MPER of gp41 has received great attention and is widely recognized as a promising target. However, little success has been achieved in eliciting MPER-specific HIV neutralizing antibodies by a number of different vaccine strategies. We investigated the ability of HA/gp41 chimeric protein-based vaccines, which were designed to enhance the exposure of the MPER in its native conformation, to induce MPER-specific HIV neutralizing antibodies. In characterization of the HA/gp41 chimeric protein, we found that by mutating an unpaired Cys residue (Cys-14 in its HA1 subunit to a Ser residue, the modified chimeric protein HA-C14S/gp41 showed increased reactivity to a conformation-sensitive monoclonal antibody against HA and formed more stable trimers in VLPs. On the other hand, HA-C14S/gp41 and HA/gp41 chimeric proteins expressed on the cell surfaces exhibited similar reactivity to monoclonal antibodies 2F5 and 4E10. Immunization of guinea pigs using the HA-C14S/gp41 DNA or VLP vaccines induced antibodies against the HIV gp41 as well as to a peptide corresponding to a segment of MPER at higher levels than immunization by standard HIV VLPs. Further, sera from vaccinated guinea pigs were found to exhibit HIV neutralizing activities. Moreover, sera from guinea pigs vaccinated by HA-C14S/gp41 DNA and VLP vaccines but not the standard HIV VLPs, were found to neutralize HIV pseudovirions containing a SIV-4E10 chimeric Env protein. The virus neutralization could be blocked by a MPER-specific peptide, thus demonstrating induction of MPER-specific HIV neutralizing antibodies by this novel vaccine strategy. These results show that induction of MPER-specific HIV neutralizing antibodies can be achieved through a rationally designed vaccine strategy.

Nur77 nuclear import and its NBRE-binding activity in thymic lymphoma cells are regulated by different mechanisms sensitive to FK506 or HA1004

International Nuclear Information System (INIS)

Kochel, Izabela; Rapak, Andrzej; Ziolo, Ewa; Strzadala, Leon

2005-01-01

Thymic lymphoma cells restore their sensitivity to ionomycin-induced apoptosis when treated with FK506 or HA1004. In apoptosis-resistant cells, ionomycin-induced Nur77 strongly binds DNA during the first 2 h of response, in contrast to lymphoma cells treated with ionomycin together with FK506 or HA1004, which undergo massive apoptosis. We show that Nur77 could discriminate between calcium signals sensitive to FK506 and those sensitive to HA1004, as the inhibitors differentially regulate the kinetics of Nur77 nuclear import, and FK506, unlike HA1004, inhibits Nur77 DNA-binding activity. In the presence of HA1004, NBRE binding by Nur77 protein increases with time (6 h vs 2 h), whereas the final outcome of both inhibitors is apoptosis of thymic lymphoma cells

Información sobre deporte femenino: el gran olvido

OpenAIRE

Ibáñez, Eugenia

2001-01-01

Las referencias al deporte femenino en los diarios de información deportiva han llegado a mínimos difícilmente superables. Prensa, radio y televisión han llegado a la conclusión por la vía de los hechos consumados de que el hombre es el único merecedor de convertirse en noticia en los medios informativos. La prensa deportiva y las secciones de deporte de los diarios de información general ignoran sistemáticamente a la mujer deportista, no informan sobre sus actividades, lo hacen mal y a desti...

LA DISTANCIA ENTRE EL DISCURSO JURÍDICO Y LA PRÁCTICA DEL ABORTO EN COLOMBIA

Directory of Open Access Journals (Sweden)

Carlos Mario Molina Betancur

2006-12-01

Full Text Available La concepción originaria de la Corte Constitucional, respecto de si el nasciturus es poseedor o no del derecho absoluto a la vida, ha ido cambiando gradualmente a medida que han ido pasando las sentencias que han estudiado el tema. No se ha llegado a la idea de considerar que el derecho a la vida se posee, al igual que todos los derechos fundamentales, desde el nacimiento, sin embargo, sí ha ido calando en la Corte la idea que el derecho a la vida del nasciturus, si es que este lo posee, no es absoluto y por eso en ocasiones debe ceder frente a los derechos de la mujer. 'El derecho al aborto y La tolerancia en el discurso iusfilosófico y la vigencia del pensamiento liberal en América Latina' son trabajos de investigación en donde se hace manifiesto el uso del término tolerancia en los diferentes sectores de poder de la sociedad y como argumento de aceptación a la diferencia, por lo tanto, para un país como Colombia, es particularmente problemático que el aborto admita una tolerancia en sentido positivo, esto porque la influencia del discurso religioso es tan fuerte que coarta las decisiones públicas aun en desmedro de las políticas de salud. Por esto se hace necesario elaborar un discurso conciliador entre el estamento político y los pronunciamientos dogmáticos de la iglesia católica en Colombia en lo que al aborto se refiere.

En busca del taíno, historia de una pelea cubana contra el normativismo

Directory of Open Access Journals (Sweden)

Daniel Torres Etayo

2008-11-01

Full Text Available Durante mucho tiempo la Arqueología caribeña ha usado ampliamente el término“Taíno” para identificar a las sociedades tribales más desarrolladas encontradas por los europeos en el momento de la conquista. Sin embargo, cuando penetramos en el profuso mundo de las investigaciones realizadas descubrimos que “lo taíno” se refiere a fenómenos distintos, dependiendo del autor, las evidencias manejadas y el país. Hasta el día de hoy, el concepto de “Cultura Taína” ha buscado, salvo raras excepciones, presentar un artificial panorama homogéneo atribuible a todos estos grupos aborígenes que poblaban las Grandes Antillas. En Cuba el caso no es diferente y el dilatado camino recorrido para encontrar una definición para la cultura o las culturas agroceramistas que ocuparon el suelo cubano, aun no ha llegado a su fin. En el trabajo se presentan las diferentes posiciones se han presentado a lo largo del tiempo, y se destacan las dos grandes líneas teóricas: la etnográfica y la particularista. También se realiza un análisis crítico de la tendencia marxista generalizadora en la Arqueología cubana y la sugerencia de algunos problemas teóricos-metodológicos a resolver desde el propio marxismo para lograr la definición, si es que existe, de la llamada cultura taina en Cuba.

Neurociencia en Psiquiatria; hacia una medicina integral y personalizada en el DSM-V: una propuesta

Directory of Open Access Journals (Sweden)

Carlos Augusto Novo Olivas

2009-01-01

Full Text Available En el último siglo la concepción de salud, y con ello de la ciencia médica, ha sufrido un cambio profundo, ontogénico, metanoico. Sin embargo, esta revolución no ha alcanzado de la misma manera a lo que hoy en día llamamos salud mental. En las siguientes páginas se tratará de mostrar, tomando como modelo al Trastorno Depresivo Mayor (TDM, la posibilidad y necesidad que tiene la psicopatología (comportamiento y capacidades “mentales”: intelectivas, racionales, cognitivas, ejecutivas, lingüísticas, emocionales, lógico-conceptuales, etc., de regresar a sus orígenes médicos, que le permitirán responder al “cómo” del funcionamiento cerebral y a su relación con el resto del organismo y con el medio ambiente, con el fin de obtener patrones que nos ayuden a definir enfermedad desde una perspectiva etiofisiopatogénica. Ha llegado el momento de cuestionar si hemos inventado una ciencia (psiquiatría que poco tiene que ver con la realidad, por lo que se propone la creación de bases de datos donde se integren variables biológicas (genéticas, cerebrales, endocrinológicas, inmunológicas, etc., sociales, personales, clínicas, etc., de forma multicéntrica e internacional, para finalmente obtener relaciones que nos permitan una reclasificación de la patología cerebral útil para proporcionar tratamientos integrales y personalizados.

1.8. Brand*, HA Badenhorst, FK 8iebrits and EH Kemm JP Hayes et ...

African Journals Online (AJOL)

Brand*, H.A. Badenhorst, F.K. 8iebrits and E.H. Kemm. Animal and Dairy Science Research Institute, Private Bag X2, Irene 1675, Republic of South Africa. J.P. Hayes. Department of Poultry Science, University of Stellenbosch, Stellenbosch 7600, Republic of South Africa. The experiment was conducted to compare the ...

Comparing response options for the International Outcome Inventory for Hearing Aids (IOI-HA) and for Alternative Interventions (IOI-AI) daily-use items.

Science.gov (United States)

Laplante-Lévesque, Ariane; Hickson, Louise; Worrall, Linda

2012-10-01

This study investigated how clients quantify use of hearing rehabilitation. Comparisons focused on the daily-use item of the International Outcome Inventory for Hearing Aids (IOI-HA), and for Alternative Interventions (IOI-AI). Adults with hearing impairment completed the original versions of the IOI-HA and the IOI-AI daily-use item which has five numerical response options (e.g. 1-4 hours/day) and a modified version with five word response options (e.g. 'Sometimes'). Respondents completed both IOI versions immediately after intervention completion and three months later. In total, 64 people who had obtained hearing aids completed both IOI-HA versions and 27 people who had participated in communication programs completed both IOI-AI versions. Participants reported higher scores on the modified (word) daily-use item than on the original (number) daily-use item. Participants who completed the IOI-AI did so significantly more than participants who completed the IOI-HA. This was true both after intervention completion and three months later. This study showed that comparisons between IOI-HA and IOI-AI daily-use item scores should be made with caution. Word daily-use response options are recommended for the IOI-AI (i.e. Never; Rarely; Sometimes; Often; and Almost always).

Evaluation of user satisfaction of hearing aids (HA in the Amazon

Directory of Open Access Journals (Sweden)

Arakawa, Aline Megumi

2010-03-01

Full Text Available Introduction: The main function of human hearing is enabling oral communication. In this sense, hearing loss impairs severely communication skills and social relationships of individuals. Therefore, the project "USP in Rondônia" of FOB/USP conducts expeditions travelling to the municipality from Monte Negro/RO allowing the promotion of hearing health. Objective: To assess the level of satisfaction user with hearing aids (HA. Method: Was accomplished a prospective study of 18 individuals with hearing loss fitted with hearing aids in the Clinic of Oral and Fono Audiological Health from Monte Negro/RO. For the evaluation, we used the questionnaire for self-assessment IOI-HA (International Outcome Inventory for Hearing Aids. Results: Concerning the seven domains assessed, it was verified that the average referring to the use was 4.2, the benefit was 3.9, the limiting of residual activity was 3.7; the satisfaction was 4.4, the restriction of participation of residual activity was 3.8, the impact on others was 4.3 and 3.9 for the quality of life. Respecting the factors one and two, it was applied the statistical test t-Student founding no statistically significant difference. However, the analysis of the score relative to factors one and two showed good results as the individual's interaction with his hearing aid and with their environment, respectively. Conclusion: With this study, we can demonstrate the high grade of satisfaction from the use of hearing aids presented by the majority of the sample collected in all domains analyzed.

Incoming human papillomavirus 16 genome is lost in PML protein-deficient HaCaT keratinocytes.

Science.gov (United States)

Bienkowska-Haba, Malgorzata; Luszczek, Wioleta; Keiffer, Timothy R; Guion, Lucile G M; DiGiuseppe, Stephen; Scott, Rona S; Sapp, Martin

2017-05-01

Human papillomaviruses (HPVs) target promyelocytic leukemia (PML) nuclear bodies (NBs) during infectious entry and PML protein is important for efficient transcription of incoming viral genome. However, the transcriptional down regulation was shown to be promoter-independent in that heterologous promoters delivered by papillomavirus particles were also affected. To further investigate the role of PML protein in HPV entry, we used small hairpin RNA to knockdown PML protein in HaCaT keratinocytes. Confirming previous findings, PML knockdown in HaCaT cells reduced HPV16 transcript levels significantly following infectious entry without impairing binding and trafficking. However, when we quantified steady-state levels of pseudogenomes in interphase cells, we found strongly reduced genome levels compared with parental HaCaT cells. Because nuclear delivery was comparable in both cell lines, we conclude that viral pseudogenome must be removed after successful nuclear delivery. Transcriptome analysis by gene array revealed that PML knockdown in clonal HaCaT cells was associated with a constitutive interferon response. Abrogation of JAK1/2 signaling prevented genome loss, however, did not restore viral transcription. In contrast, knockdown of PML protein in HeLa cells did not affect HPV genome delivery and transcription. HeLa cells are transformed by HPV18 oncogenes E6 and E7, which have been shown to interfere with the JAK/Stat signaling pathway. Our data imply that PML NBs protect incoming HPV genomes. Furthermore, they provide evidence that PML NBs are key regulators of the innate immune response in keratinocytes. Promyelocytic leukemia nuclear bodies (PML NBs) are important for antiviral defense. Many DNA viruses target these subnuclear structures and reorganize them. Reorganization of PML NBs by viral proteins is important for establishment of infection. In contrast, HPVs require the presence of PML protein for efficient transcription of incoming viral genome. Our

In vitro cytotoxicity and in vivo osseointergration properties of compression-molded HDPE-HA-Al2O3 hybrid biocomposites.

Science.gov (United States)

Tripathi, Garima; Gough, Julie E; Dinda, Amit; Basu, Bikramjit

2013-06-01

The aim of this study was to investigate the in vivo biocompatibility in terms of healing of long segmental bone defect in rabbit model as well as in vitro cytotoxicity of eluates of compression-molded High density polyethylene (HDPE)-hydroxyapatite (HA)-aluminum oxide (Al2O3) composite-based implant material. Based on the physical property in terms of modulus and strength properties, as reported in our recent publication, HDPE-40 wt % HA and HDPE-20 wt % HA-20 wt % Al2O3 hybrid composites were used for biocompatibility assessment. Osteoblasts cells were cultured in conditioned media, which contains varying amount of composite eluate (0.01, 0.1, and 1.0 wt %). In vitro, the eluates did not exhibit any significant negative impact on proliferation, mineralization or on morphology of human osteoblast cells. In vivo, the histological assessment revealed neobone formation at the bone/implant interface, characterized by the presence of osteoid and osteoblasts. The observation of osteoclastic activity indicates the process of bone remodeling. No inflammation to any noticeable extent was observed at the implantation site. Overall, the combination of in vitro and in vivo results are suggestive of potential biomedical application of compression-molded HDPE- 20 wt % HA- 20 wt % Al2O3 composites to heal long segmental bone defects without causing any toxicity of bone cells. Copyright © 2012 Wiley Periodicals, Inc.

7 CFR 1901.505 - Certificates of beneficial ownership in FmHA or its successor agency under Public Law 103-354 loans.

Science.gov (United States)

2010-01-01

... ownership in FmHA or its successor agency under Public Law 103-354 loans. (a) Special trust of loans—(1) Establishment of special trusts. From time to time FmHA or its successor agency under Public Law 103-354 will... successor agency under Public Law 103-354 will own an interest in special trusts equal to the amount by...

7 CFR 1955.12 - Acquisition of property which served as security for a loan guarantee by FmHA or its successor...

Science.gov (United States)

2010-01-01

... Chattel Property § 1955.12 Acquisition of property which served as security for a loan guarantee by FmHA... 7 Agriculture 14 2010-01-01 2009-01-01 true Acquisition of property which served as security for a loan guarantee by FmHA or its successor agency under Public Law 103-354 or at sale by another...

Què ha de tenir en compte l'usuari d'ordinador?

OpenAIRE

Valls Oriol, Maria Mercè

2014-01-01

El treball que aquí es presenta és la creació d'un curs online pilot amb la plataforma online i gratuïta Chamilo per realitzar formació en l'empresa Consorci Sanitari (que no té plataforma virtual d'aprenentatge implantada). La temàtica del curs es basa en tot el que fa referència a l'ergonomia relacionada amb la utilització dels ordinadors. La creació del curs s'ha realitzat amb la metodologia ADDIE obtenint resultats positius, fet que conclou que la implementació d'una plataforma virtual d'...

Spectroscopic analyses on sonocatalytic damage to bovine serum albumin (BSA) induced by ZnO/hydroxylapatite (ZnO/HA) composite under ultrasonic irradiation

Science.gov (United States)

Wang, Zhiqiu; Li, Ying; Wang, Jun; Zou, Mingming; Gao, Jingqun; Kong, Yumei; Li, Kai; Han, Guangxi

ZnO/hydroxylapatite (ZnO/HA) composite with HA molar content of 6.0% was prepared by the method of precipitation and heat-treated at 500 °C for 40 min and was characterized by powder X-ray diffraction (XRD). The sonocatalytic activities of ZnO/HA composite was carried out through the damage of bovine serum albumin (BSA) in aqueous solution. Furthermore, the effects of several factors on the damage of BSA molecules were evaluated by means of UV-vis and fluorescence spectra. Experimental results indicated that the damage degree of BSA aggravated with the increase of ultrasonic irradiation time, irradiation power and ZnO/HA addition amount, but weakened with the increase of solution acidity and ionic strength. In addition, the damage site to BSA was also studied by synchronous fluorescence technology and the damage site was mainly at tryptophan (Trp) residue. This paper provides a valuable reference for driving sonocatalytic method to treat tumor in clinic application.

¿Ha mejorado la calidad de los comedores escolares españoles?

OpenAIRE

Martínez Pérez, Naiara

2016-01-01

El aumento de la prevalencia de la obesidad infantil debido a la crisis financiera y en consecuencia, a la transición nutricional, ha aumentado la preocupación por los alimentos consumidos en los comedores escolares españoles. The increasing prevalence of childhood obesity due to the financial crisis and consequently the nutrition transition, has increased concern for Spanish food consumed in school canteens. L'augment de la prevalença de l'obesitat infantil a causa de la crisi financer...

Are Women Empowered to Save?

Directory of Open Access Journals (Sweden)

Frances Woolley

2013-12-01

Full Text Available Female economic empowerment – rising earnings, increased opportunities, greater labour force participation – has given many women the means to save. The shifting of responsibility for retirement security from employers and governments onto individuals has given women a reason to save. But are women actually saving? In this paper, we explore the relationship between the gender dynamics within a family and the accumulation of wealth. We find that little evidence in support of the conventional wisdom that families with a female financial manager save more and repay their debts more often. We find some evidence that male financial management leads to greater savings, and other evidence suggesting that savings patterns have a complex relationship with intra-family gender dynamics. El empoderamiento económico de la mujer – el aumento de los ingresos, mayores oportunidades, mayor participación laboral – ha dado a muchas mujeres los medios para ahorrar. Al pasar la responsabilidad de los ingresos de la jubilación de los empleadores y el gobierno a los individuos ha dado a las mujeres un motivo para ahorrar. ¿Pero realmente ahorran las mujeres? En este artículo se analizan las relaciones entre las dinámicas de género en una familia, y la acumulación de riqueza. Se ha llegado a la conclusión de que hay poca evidencia que apoye la creencia convencional de que las familias en las que una mujer gestiona las financias ahorran más y devuelven sus créditos más frecuentemente. Se ha encontrado alguna evidencia de que la gestión financiera por varones acarrea mayores ahorros, y otras evidencias que sugieren que los patrones de ahorro tienen una relación compleja con las dinámicas de género dentro de la familia.

Universitarias en España: De los inicios a la actualidad

Directory of Open Access Journals (Sweden)

Ana Guil Bozal

2015-01-01

Full Text Available En este artículo se ofrece un análisis de la evolución cuantitativa de la presencia de mujeres en las universidades españolas, desde las pioneras decimonónicas hasta el comienzo del nuevo milenio. Partiendo de la hipótesis de que el contexto social y la mentalidad generalizada acerca de la condición femenina no favorecían ese tipo de decisiones, el objetivo es dar a conocer el evidente y progresivo crecimiento en las aulas universitarias, primero de alumnas y más tarde de profesoras. La metodología utilizada ha partido de una revisión de la bibliografía disponible sobre esta temática, no muy abundante pero sí precisa en los datos e informaciones que ofrece; a continuación se ha buscado en las fuentes primarias que custodian los archivos universitarios y de la administración del Estado, en las series históricas de los anuarios estadísticos de alcance nacional e internacional. La síntesis, contraste y sistematización de todos los datos localizados nos ha permitido cubrir la finalidad prevista y ofrecer una imagen diacrónica de las mujeres que eligieron la universidad como proyecto primero de formación y luego profesional. La lectura de estas páginas permite comprobar algunas de las características de una cadencia temporal de largo plazo, casi ciento cincuenta años, y de la situación a la que se ha llegado a comienzos del siglo XXI, en que aún no se han logrado auténticas y reales oportunidades de igualdad en los ámbitos universitarios.

Insights into the interaction between nucleoid-associated proteins H ha and H-NS by NMR and fluorescence anisotropy

International Nuclear Information System (INIS)

Cordeiro, T.N.; Garcia, J.; Pons, M.

2005-01-01

NMR and fluorescence anisotropy are both valuable tools for studying bio molecular interactions. NMR can provide structural insights at atomic resolution. Still, it can be wisely complemented by lower-resolution biophysical techniques, such as fluorescence anisotropy. In this article we report the combination of NMR and fluorescence anisotropy in establishing novel structure-function insights into the interaction between two bacterial nucleoid-associated proteins, H ha and H-NS. H ha (H-NS) complexes are known to play an important role in modulating the expression of some environmentally regulated genes that confer survival advantage in a particular growth condition. (author)

Insights into the interaction between nucleoid-associated proteins H ha and H-NS by NMR and fluorescence anisotropy

Energy Technology Data Exchange (ETDEWEB)

Cordeiro, T.N.; Garcia, J. [Institut de Recerca Biomedica-Parc Cientific de (Spain). Lab. of Biomolecular NMR; Pons, M. [Universitat de Barcelona (Spain). Dept. de Quimica Organica]. E-mail: mpons@ub.edu

2005-07-01

NMR and fluorescence anisotropy are both valuable tools for studying bio molecular interactions. NMR can provide structural insights at atomic resolution. Still, it can be wisely complemented by lower-resolution biophysical techniques, such as fluorescence anisotropy. In this article we report the combination of NMR and fluorescence anisotropy in establishing novel structure-function insights into the interaction between two bacterial nucleoid-associated proteins, H ha and H-NS. H ha (H-NS) complexes are known to play an important role in modulating the expression of some environmentally regulated genes that confer survival advantage in a particular growth condition. (author)

Endiguer la vague de criminalité en Haïti | CRDI - Centre de ...

International Development Research Centre (IDRC) Digital Library (Canada)

17 avr. 2012 ... En Haïti, les signes de danger sautent littéralement aux yeux. Dans nombre des quartiers de Port‑au‑Prince dont les murs sont couverts de graffitis, on observe une grave escalade de la violence meurtrière. La montée en flèche du nombre de meurtres, de crimes contre les biens et de voies de fait, qui s'est ...

Mucosal and Systemic Immune Responses to Influenza H7N9 Antigen HA1-2 Co-Delivered Intranasally with Flagellin or Polyethyleneimine in Mice and Chickens.

Science.gov (United States)

Song, Li; Xiong, Dan; Song, Hongqin; Wu, Lili; Zhang, Meihua; Kang, Xilong; Pan, Zhiming; Jiao, Xinan

2017-01-01

Consecutive cases of human infection with H7N9 influenza viruses since 2013 in China have prompted efforts to develop an effective treatment. Subunit vaccines introduced by intranasal administration can block an infection at its primary site; flagellin (fliC) and polyethyleneimine (PEI) have been shown to be potent adjuvants. We previously generated the hemagglutinin (HA)1-2-fliC fusion protein consisting of the globular head domain (HA1-2; amino acids 62-284) of HA fused with Salmonella typhimurium fliC. In the present study, we investigated its effectiveness of both flagellin and PEI as mucosal adjuvants for the H7N9 influenza subunit vaccine. Mice immunized intranasally with HA1-2-fliC and HA1-2-PEI showed higher HA1-2-specific immunoglobulin (Ig)G and IgA titers in serum, nasal wash, and bronchial alveolar lavage fluid. Moreover, splenocyte activation and proliferation and the number of HA1-2-specific interferon (IFN)-γ- and interleukin (IL)-4-producing splenocytes were markedly increased in the fliC and PEI groups; in the latter, there were more cells secreting IL-4 than IFN-γ, suggesting that fliC induced T helper type (Th)1 and Th2 immune responses, and PEI induced Th2-biased responses, consistent with the serum antibody isotype pattern (IgG1/IgG2a ratio). Furthermore, virus challenge was performed in a chicken model. The results showed that chickens receiving fliC and PEI adjuvant vaccine exhibited robust immune responses leading to a significant reduction in viral loads of throat and cloaca compared to chickens receiving only HA1-2. These findings provide a basis for the development of H7N9 influenza HA1-2 mucosal subunit vaccines.

In vitro and in vivo evaluations of nano-hydroxyapatite/polyamide 66/glass fibre (n-HA/PA66/GF as a novel bioactive bone screw.

Directory of Open Access Journals (Sweden)

Bao Su

Full Text Available In this study, we prepared nano-hydroxyapatite/polyamide 66/glass fibre (n-HA/PA66/GF bioactive bone screws. The microstructure, morphology and coating of the screws were characterised, and the adhesion, proliferation and viability of MC3T3-E1 cells on n-HA/PA66/GF scaffolds were determined using scanning electron microscope, CCK-8 assays and cellular immunofluorescence analysis. The results confirmed that n-HA/PA66/GF scaffolds were biocompatible and had no negative effect on MC3T3-E1 cells in vitro. To investigate the in vivo biocompatibility, internal fixation properties and osteogenesis of the bioactive screws, both n-HA/PA66/GF screws and metallic screws were used to repair intercondylar femur fractures in dogs. General photography, CT examination, micro-CT examination, histological staining and biomechanical assays were performed at 4, 8, 12 and 24 weeks after operation. The n-HA/PA66/GF screws exhibited good biocompatibility, high mechanical strength and extensive osteogenesis in the host bone. Moreover, 24 weeks after implantation, the maximum push-out load of the bioactive screws was greater than that of the metallic screws. As shown by their good cytocompatibility, excellent biomechanical strength and fast formation and ingrowth of new bone, n-HA/PA66/GF screws are thus suitable for orthopaedic clinical applications.

Calcitriol increases Dicer expression and modifies the microRNAs signature in SiHa cervical cancer cells.

Science.gov (United States)

González-Duarte, Ramiro José; Cázares-Ordoñez, Verna; Romero-Córdoba, Sandra; Díaz, Lorenza; Ortíz, Víctor; Freyre-González, Julio Augusto; Hidalgo-Miranda, Alfredo; Larrea, Fernando; Avila, Euclides

2015-08-01

MicroRNAs play important roles in cancer biology. Calcitriol, the hormonal form of vitamin D3, regulates microRNAs expression in tumor cells. In the present study we asked if calcitriol would modify some of the components of the microRNA processing machinery, namely, Drosha and Dicer, in calcitriol-responsive cervical cancer cells. We found that calcitriol treatment did not affect Drosha mRNA; however, it significantly increased Dicer mRNA and protein expression in VDR-positive SiHa and HeLa cells. In VDR-negative C33-A cells, calcitriol had no effect on Dicer mRNA. We also found a vitamin D response element in Dicer promoter that interacts in vitro to vitamin D and retinoid X receptors. To explore the biological plausibility of these results, we asked if calcitriol alters the microRNA expression profile in SiHa cells. Our results revealed that calcitriol regulates the expression of a subset of microRNAs with potential regulatory functions in cancer pathways, such as miR-22, miR-296-3p, and miR-498, which exert tumor-suppressive effects. In summary, the data indicate that in SiHa cells, calcitriol stimulates the expression of Dicer possibly through the vitamin D response element located in its promoter. This may explain the calcitriol-dependent modulation of microRNAs whose target mRNAs are related to anticancer pathways, further adding to the various anticancer mechanisms of calcitriol.

MREIT conductivity imaging of the postmortem canine abdomen using CoReHA

International Nuclear Information System (INIS)

Jeon, Kiwan; Lee, Chang-Ock; Minhas, Atul S; Kim, Young Tae; Jeong, Woo Chul; Kim, Hyung Joong; Woo, Eung Je; Kang, Byeong Teck; Park, Hee Myung; Seo, Jin Keun

2009-01-01

Magnetic resonance electrical impedance tomography (MREIT) is a new bio-imaging modality providing cross-sectional conductivity images from measurements of internal magnetic flux densities produced by externally injected currents. Recent experimental results of postmortem and in vivo imaging of the canine brain demonstrated its feasibility by showing conductivity images with meaningful contrast among different brain tissues. MREIT image reconstructions involve a series of data processing steps such as k-space data handling, phase unwrapping, image segmentation, meshing, modelling, finite element computation, denoising and so on. To facilitate experimental studies, we need a software tool that automates these data processing steps. In this paper, we summarize such an MREIT software package called CoReHA (conductivity reconstructor using harmonic algorithms). Performing imaging experiments of the postmortem canine abdomen, we demonstrate how CoReHA can be utilized in MREIT. The abdomen with a relatively large field of view and various organs imposes new technical challenges when it is chosen as an imaging domain. Summarizing a few improvements in the experimental MREIT technique, we report our first conductivity images of the postmortem canine abdomen. Illustrating reconstructed conductivity images, we discuss how they discern different organs including the kidney, spleen, stomach and small intestine. We elaborate, as an example, that conductivity images of the kidney show clear contrast among cortex, internal medulla, renal pelvis and urethra. We end this paper with a brief discussion on future work using different animal models

Quantitative Analysis of the Mineralogical Stages of the Clinkers of Portland Cement. Qualitative and Quantitative Analysis, by Infrared Spectroscopy of the Mineralogical Stages of the Clinker of Portland Cement

Directory of Open Access Journals (Sweden)

Käsner, Bernd

1972-09-01

Full Text Available Not availablePara la determinación cuantitativa de las fases mineralógicas del clinker de cemento portland, tan sólo se emplean el análisis microscópico y el de difracción de rayos X. Ambas técnicas exigen una preparación de muestras muy cuidadosa, lo que lleva bastante tiempo. A causa de estas circunstancias surge el interés de desarrollar un procedimiento con el que se pueda obtener resultados de exactitud y con poco trabajo, basado en la espectroscopia infrarroja. En principio existen dificultades, tanto en el método de trabajo como de interpretación de resultados. Por ejemplo, la formación y composición de las fases del clíñker se hallan sometidas a variaciones; por otra parte, aún no se ha llegado a elaborar un procedimiento para estudio por IR de sistemas que contengan varios componentes inorgánicos en estado sólido. Se ha pensado en la realización previa de análisis elementales, con los que se aclararían los de las fases minerales de los clínkeres, ya que no está aún suficientemente resuelto —a pesar de la importancia que esto representa— el análisis cuantitativo de estas fases.

LOS EXTRANJEROS RESIDENTES EN ESPAÑA: SU APORTACIÓN A LA DEMOGRAFÍA

Directory of Open Access Journals (Sweden)

Vicente Gozálvez Pérez

2010-01-01

Full Text Available La inmigración extranjera en España ha acumulado stocks muy importantes durante la última década, en su mayoría con vocación de permanencia en el país. Con ello la población de España ha crecido con las tasas más altas de su historia, apoyadas en la inmigración neta. La aportación más importante de los inmigrados a la demografía de España es su rejuvenecimiento, pues los inmigrados menores de 40 años de edad superan las tres cuartas partes de su total. Los recién llegados son adultos-jóvenes, mientras la población infantil crece mucho por su doble alimentación: reagrupación familiar y los nacidos en España de madre extranjera, lo que en parte corrige el desequilibrio producido por la persistente desnatalidad del país. Por último se analizan algunas aportaciones de los trabajadores extranjeros al mercado laboral nacional: irregularidad laboral, tasas de paro muy altas y ocupación de empleos menos valorados o rechazados por los españoles son algunas de sus características, que, en cualquier caso, dificultan la integración actual y futura de estos extranjeros en España.

Desde la elevada meseta mexicana: con una larga-vista demográfica.

Directory of Open Access Journals (Sweden)

Raúl Benítez Zenteno

2001-01-01

Full Text Available En la segunda mitad del siglo XX, contrario a los discursos políticos y algunas teorías, en los países pobres el menor crecimiento demográfico inducido por las políticas internacionales no se reflejó en crecimiento económico y mejores condiciones de vida. Cuestión de la que se deriva la interrogante de cuál ha sido nuestra participación en la toma de decisiones orientadas a ampliar el bienestar. Posteriormente, se expone lo que han sido las áreas de investigación en materia de población y se realiza un balance sobre las "modas" que han llegado del exterior, señalando que, en general, el saldo ha sido negativo, principalmente por su adopción acrítica, la falta de creatividad teórica propia y la poca profundidad en la explicación de interrelaciones entre los factores demográficos, socioeconómicos, políticos y culturales. Para concluir, se hace una revisión de la práctica demográfica en México, en la que resalta la falta de demógrafos bien formados, la persistencia de explicaciones reducidas, la poca colaboración grupal e interinstitucional, y la ausencia de recursos.

El régimen impositivo simplificado ecuatoriano Rise: desenvolvimiento y perspectivas

Directory of Open Access Journals (Sweden)

José Díaz Montenegro

2013-12-01

Full Text Available El objetivo del presente trabajo es determinar el comportamiento que ha tenido a través del tiempo el Régimen Impositivo Simplificado Ecuatoriano RISE, en cuanto generador importante de recursos para el Estado, cumpliendo así con los propósitos por los cuales fue creado en el año 2008. Para ello se utiliza estadística descriptiva en función de datos emitidos por el Servicio de Rentas Internas SRI. Las aplicaciones realizadas permiten determinar, que si bien la recaudación proporcionada por este Régimen Tributario muestra un comportamiento creciente en términos monetarios, su dinamia en valores relativos ha llegado a un aletargamiento peligroso, motivado en gran parte por el descuido que su operatividad muestra, como resultado principalmente de las escasas campañas de difusión, así como de la disminución de los mecanismos de control por parte de la Administración Tributaria. Se concluye que es imperiosa la necesidad de reactivar esta modalidad impositiva, más aún si tomamos en cuenta que su cumplimiento es también un indicativo del grado de informalidad –o economía subterránea- en que se desenvuelven determinadas actividades productivas. PALABRAS CLAVE: Impuesto a la renta, impuesto al valor agregado, ley de equidad tributaria.

24.000 ha de păduri primare de fag, propunerea României pentru Patrimoniul Mondial UNESCO [24000 ha of primary beech forests, the Romanian proposal in UNESCO World Heritage

Directory of Open Access Journals (Sweden)

Biriș I.-A.

2016-08-01

Full Text Available Under the coordination of The Ministry of Environment, Waters and Forests, about 24000 ha of primeval beech forests located in 8 natural protected area from the Romanian Carpathians were included for inscription into the World Heritage List to extend the „Primeval Beech Forests of the Carpathians and the Ancient Beech Forests of Germany” (1133bis. The extended World Heritage property is proposed to carry the joint new name: „Primeval Beech Forests of the Carpathians and Other Regions of Europe”. Supported by 13 European countries (Romania included, the nomination proposes to establish a transnational serial UNESCO property, with a surface of more that 95000 ha, that reunites the most representative and well preserved beech forests from the natural areal of this species in order to illustrate the ecological process of extension - that is in progress at present - of beech on the European continent. The process of choosing these canditate sites was thought for completing the existent sites and for providing arguments and reflecting better on the extension of species distribution at the level of Europe. At the end of January 2016, Austria, the coordinator country of the nomination process at the international level transmitted the common dossier for the nomination to the committee of evaluation of UNESCO World Heritage List.

Broad cross-reactive IgG responses elicited by adjuvanted vaccination with recombinant influenza hemagglutinin (rHA) in ferrets and mice

Science.gov (United States)

Wang, Jiong; Hilchey, Shannon P.; DeDiego, Marta; Perry, Sheldon; Hyrien, Ollivier; Nogales, Aitor; Garigen, Jessica; Amanat, Fatima; Huertas, Nelson; Krammer, Florian; Martinez-Sobrido, Luis; Topham, David J.; Treanor, John J.; Sangster, Mark Y.

2018-01-01

Annual immunization against influenza virus is a large international public health effort. Accumulating evidence suggests that antibody mediated cross-reactive immunity against influenza hemagglutinin (HA) strongly correlates with long-lasting cross-protection against influenza virus strains that differ from the primary infection or vaccination strain. However, the optimal strategies for achieving highly cross-reactive antibodies to the influenza virus HA have not yet to be defined. In the current study, using Luminex-based mPlex-Flu assay, developed by our laboratory, to quantitatively measure influenza specific IgG antibody mediated cross-reactivity, we found that prime-boost-boost vaccination of ferrets with rHA proteins admixed with adjuvant elicited higher magnitude and broader cross-reactive antibody responses than that induced by actual influenza viral infection, and this cross-reactive response likely correlated with increased anti-stalk reactive antibodies. We observed a similar phenomenon in mice receiving three sequential vaccinations with rHA proteins from either A/California/07/2009 (H1N1) or A/Hong Kong/1/1968 (H3N2) viruses admixed with Addavax, an MF59-like adjuvant. Using this same mouse vaccination model, we determined that Addavax plays a more significant role in the initial priming event than in subsequent boosts. We also characterized the generation of cross-reactive antibody secreting cells (ASCs) and memory B cells (MBCs) when comparing vaccination to viral infection. We have also found that adjuvant plays a critical role in the generation of long-lived ASCs and MBCs cross-reactive to influenza viruses as a result of vaccination with rHA of influenza virus, and the observed increase in stalk-reactive antibodies likely contributes to this IgG mediated broad cross-reactivity. PMID:29641537

Synergistic augmentation of ATP-induced interleukin-6 production by arsenite in HaCaT cells.

Science.gov (United States)

Sumi, Daigo; Asao, Masashi; Okada, Hideta; Yogi, Kuniko; Miyataka, Hideki; Himeno, Seiichiro

2016-06-01

Chronic arsenic exposure causes cutaneous diseases such as hyperkeratosis and skin cancer. However, little information has been available regarding the molecular mechanisms underlying these symptoms. Because extracellular ATP and interleukin-6 (IL-6) are involved in pathological aspects of cutaneous diseases, we examined whether sodium arsenite (As(III)) affects ATP-induced IL-6 production in human epidermal keratinocyte HaCaT cells. The results showed that the addition of As(III) into the medium of HaCaT cells dose dependently increased the production of IL-6 induced by extracellular ATP, although As(III) alone had no effect on IL-6 production. To elucidate the mechanism of the synergistic effect of As(III) on IL-6 production by extracellular ATP, we next examined the phosphorylation of p38, ERK and epidermal growth factor receptor (EGFR), since we found that these signaling molecules were stimulated by exposure to extracellular ATP. The results indicated that ATP-induced phosphorylation of p38, ERK and EGFR was synergistically enhanced by co-exposure to As(III). To clarify the mechanisms underlying the enhanced phosphorylation of p38, ERK and EGFR by As(III), we explored two possible mechanisms: the inhibition of extracellular ATP degradation and the inhibition of protein tyrosine phosphatases (PTPs) activity by As(III). The degradation of extracellular ATP was not changed by As(III), whereas the activity of PTPs was significantly inhibited by As(III). Our results suggest that As(III) augments ATP-induced IL-6 production in HaCaT cells through enhanced phosphorylation of the EGFR and p38/ERK pathways, which is associated with the inhibition of PTPs activity.

Les stratégies spatiales de la population haïtienne à Miami

Directory of Open Access Journals (Sweden)

Cédric Audebert

2008-02-01

Full Text Available Le schéma migratoire haïtien s’est traduit par la genèse d’un champ migratoire international entre le pays d’origine et Miami, où la population d’origine haïtienne a enregistré une croissance rapide. Dans une métropole floridienne marquée par une forte ségrégation « ethno-raciale » et socio-économique, les nouveaux venus se sont installés à l’origine dans les quartiers noirs centraux paupérisés. Les incidences de la politique migratoire fédérale sont apparues contrastées, occasionnant le repli sur soi des immigrants à Little Haiti lorsqu’elle était défavorable ou rendant possible la mobilité résidentielle dans une conjoncture d’assouplissement de la législation. Ultérieurement, la complexité croissante de l’espace de l’immigration haïtienne, caractérisé par une évolution rapide de son étendue et de ses formes, s’est manifestée par une dichotomie marquée entre l’« enclave » traditionnelle et les nouveaux espaces d’installation en banlieue. Résultat d’une dynamique d’agrégation autant choisie que contrainte, la concentration spatiale des Haïtiens à Miami s’est traduite par la genèse de secteurs d’implantation privilégiée, terrains favorables à la mise en place de processus de territorialisation complexes. L’apprentissage mental et fonctionnel de l’espace vise à faire sienne la terre d’immigration et à la doter de sens, et apparaît comme le préalable à la mise en place de stratégies collectives de territorialisation. La « prise de possession » d’une portion de l’aire métropolitaine et son affectation à des activités sociales, commerciales et de représentation politique visent à assurer la pérennité du lien communautaire haïtien en créant de la cohésion, de la solidarité et de la socialité.Haitian emigration has evolved within the growing dependency of Haiti from the United States throughout the XXth century. The Haitian migratory pattern

Radiochemistry, pre-clinical studies and first clinical investigation of 90Y-labeled hydroxyapatite (HA) particles prepared utilizing 90Y produced by (n,γ) route

International Nuclear Information System (INIS)

Vimalnath, K.V.; Chakraborty, Sudipta; Rajeswari, A.; Sarma, H.D.; Nuwad, Jitendra; Pandey, Usha; Kamaleshwaran, K.; Shinto, Ajit; Dash, Ashutosh

2015-01-01

Introduction: The scope of using no carrier added (NCA) 90 Y [T 1/2 = 64.1 h, E β(max) = 2.28 MeV] obtained from 90 Sr/ 90 Y generator in radiation synovectomy (RSV) is widely accepted. In the present study, the prospect of using 90 Y produced by (n,γ) route in a medium flux research reactor for use in RSV was explored. Methods: Yttrium-90 was produced by thermal neutron irradiation of Y 2 O 3 target at a neutron flux of ~ 1 × 10 14 n/cm 2 .s for 14 d. The influence of various experimental parameters were systematically investigated and optimized to arrive at the most favorable conditions for the formulation of 90 Y labeled hydroxyapatite (HA) using HA particles of 1–10 μm size range. An optimized kit formulation strategy was developed for convenient one-step compounding of 90 Y-HA, which is easily adaptable at hospital radiopharmacy. The pre-clinical biological evaluation of 90 Y-HA particles was studied by carrying out biodistribution and bioluminiscence imaging studies in Wistar rats. The first clinical investigation using the radiolabeled preparation was performed on a patient suffering from chronic arthritis in knee joint by administering 185 MBq 90 Y-HA formulated at the hospital radiopharmacy deploying the proposed strategy. Results: Yttrium-90 was produced with a specific activity of 851 ± 111 MBq/mg and radionuclidic purity of 99.95 ± 0.02%. 90 Y-labeled HA particles (185 ± 10 MBq doses) were formulated in high radiochemical purity (> 99%) and excellent in vitro stability. The preparation showed promising results in pre-clinical studies carried out in Wistar rats. The preliminary results of the first clinical investigation of 90 Y-HA preparation in a patient with rheumatoid arthritis in knee joints demonstrated the effectiveness of the formulation prepared using 90 Y produced via (n,γ) route in the management of the disease. Conclusion: The studies revealed that effective utilization of 90 Y produced via (n,γ) route in a medium flux research

In vitro and in vivo performance of bioactive Ti6Al4V/TiC/HA implants fabricated by a rapid microwave sintering technique.

Science.gov (United States)

Choy, Man Tik; Tang, Chak Yin; Chen, Ling; Wong, Chi Tak; Tsui, Chi Pong

2014-09-01

Failure of the bone-implant interface in a joint prosthesis is a main cause of implant loosening. The introduction of a bioactive substance, hydroxyapatite (HA), to a metallic bone-implant may enhance its fixation on human bone by encouraging direct bone bonding. Ti6Al4V/TiC/HA composites with a reproducible porous structure (porosity of 27% and pore size of 6-89 μm) were successfully fabricated by a rapid microwave sintering technique. This method allows the biocomposites to be fabricated in a short period of time under ambient conditions. Ti6Al4V/TiC/HA composites exhibited a compressive strength of 93 MPa, compressive modulus of 2.9 GPa and microhardness of 556 HV which are close to those of the human cortical bone. The in vitro preosteoblast MC3T3-E1 cells cultured on the Ti6Al4V/TiC/HA composite showed that the composite surface could provide a biocompatible environment for cell adhesion, proliferation and differentiation without any cytotoxic effects. This is among the first attempts to study the in vivo performance of load-bearing Ti6Al4V/TiC and Ti6Al4V/TiC/HA composites in a live rabbit. The results indicated that the Ti6Al4V/TiC/HA composite had a better bone-implant interface compared with the Ti6Al4V/TiC implant. Based on the microstructural features, the mechanical properties, and the in vitro and in vivo test results from this study, the Ti6Al4V/TiC/HA composites have the potential to be employed in load-bearing orthopedic applications. Copyright © 2014 Elsevier B.V. All rights reserved.

The Proteasome Inhibitor MG-132 Protects Hypoxic SiHa Cervical Carcinoma Cells after Cyclic Hypoxia/Reoxygenation from Ionizing Radiation

Directory of Open Access Journals (Sweden)

Frank Pajonk

2006-12-01

Full Text Available INTRODUCTION: Transient hypoxia and subsequent reoxygenation are common phenomena in solid tumors that greatly influence the outcome of radiation therapy. This study was designed to determine how varying cycles of hypoxia/reoxygenation affect the response of cervical carcinoma cells irradiated under oxic and hypoxic conditions and whether this could be modulated by proteasome inhibition. MATERIALS AND METHODS: Plateau-phase SiHa cervical carcinoma cells in culture were exposed to varying numbers of 30-minute cycles of hypoxia/reoxygenation directly before irradiation under oxic or hypoxic conditions. 26S Proteasome activity was blocked by addition of MG-132. Clonogenic survival was measured by a colonyforming assay. RESULTS: Under oxic conditions, repeated cycles of hypoxia/reoxygenation decreased the clonogenic survival of SiHa cells. This effect was even more pronounced after the inhibition of 26S proteasome complex. In contrast, under hypoxic conditions, SiHa cells were radioresistant, as expected, but this was increased by proteasome inhibition. CONCLUSIONS: Proteasome inhibition radiosensitizes oxygenated tumor cells but may also protect tumor cells from ionizing radiation under certain hypoxic conditions.

Obsolescencia programada como fenómeno cultural real

Directory of Open Access Journals (Sweden)

Liliana Karina Niño Rubio

2015-01-01

Full Text Available Los modelos económicos han mutado a través del tiempo dependiendo de las necesidades sociales, históricas y culturales; ya haya sido los esclavos, la tierra o el dinero, siempre ha habido un factor claro que se ha mantenido. Quienes tuvieron el poder desde el primer modelo económico son los mismos que lo poseen en la actualidad, al igual que quienes vivieron bajo su yugo seguimos siendo los mismos. A pesar de los cambios sufridos por el modelo económico reinante, el que más ha perdurado y nos acompaña hasta nuestros días es el capitalismo, el cual nació gracias a la industrialización, los avances tecnológicos y la adquisición de bienes de consumo. En teoría es un modelo sólido y funcional que ha subsistido bastante tiempo, sin embargo, este ha llegado a convertirse en un problema social, económico, cultural y ecológico. La sociedad de consumo masivo en la que nos hemos convertido, no solo puede llegar a generar escalofríos, sino que también está generando un gran daño a nuestro medio ambiente; solo en Estados Unidos cada habitante genera en promedio 2,3 kg de basura al día; o, por ejemplo, en el mar Caspio, situado al Norte de Irán y al extremo sur del Cáucaso con 374 000 km2 (el mar cerrado más grande del mundo recoge las aguas servidas y la contaminación de una de las regiones más pobladas e industrializadas de Rusia a través del río Volga, el más importante de los 130 cursos de agua que lo alimentan. Al comienzo de los años 90, el nivel de contaminación del Volga era considerado realmente alarmante y sigue empeorando desde entonces.

Wide-range stiffness gradient PVA/HA hydrogel to investigate stem cell differentiation behavior.

Science.gov (United States)

Oh, Se Heang; An, Dan Bi; Kim, Tae Ho; Lee, Jin Ho

2016-04-15

Although stiffness-controllable substrates have been developed to investigate the effect of stiffness on cell behavior and function, the use of separate substrates with different degrees of stiffness, substrates with a narrow range stiffness gradient, toxicity of residues, different surface composition, complex fabrication procedures/devices, and low cell adhesion are still considered as hurdles of conventional techniques. In this study, a cylindrical polyvinyl alcohol (PVA)/hyaluronic acid (HA) hydrogel with a wide-range stiffness gradient (between ∼20kPa and ∼200kPa) and cell adhesiveness was prepared by a liquid nitrogen (LN2)-contacting gradual freezing-thawing method that does not use any additives or specific devices to produce the stiffness gradient hydrogel. From an in vitro cell culture using the stiffness gradient PVA/HA hydrogel, it was observed that human bone marrow mesenchymal stem cells have favorable stiffness ranges for induction of differentiation into specific cell types (∼20kPa for nerve cell, ∼40kPa for muscle cell, ∼80kPa for chondrocyte, and ∼190kPa for osteoblast). The PVA/HA hydrogel with a wide range of stiffness spectrum can be a useful tool for basic studies related with the stem cell differentiation, cell reprogramming, cell migration, and tissue regeneration in terms of substrate stiffness. It is postulated that the stiffness of the extracellular matrix influences cell behavior. To prove this concept, various techniques to prepare substrates with a stiffness gradient have been developed. However, the narrow ranges of stiffness gradient and complex fabrication procedures/devices are still remained as limitations. Herein, we develop a substrate (hydrogel) with a wide-range stiffness gradient using a gradual freezing-thawing method which does not need specific devices to produce a stiffness gradient hydrogel. From cell culture experiments using the hydrogel, it is observed that human bone marrow mesenchymal stem cells have

Evaluation of the immunogenicity and protective effects of a trivalent chimeric norovirus P particle immunogen displaying influenza HA2 from subtypes H1, H3 and B.

Science.gov (United States)

Gong, Xin; Yin, He; Shi, Yuhua; He, Xiaoqiu; Yu, Yongjiao; Guan, Shanshan; Kuai, Ziyu; Haji, Nasteha M; Haji, Nafisa M; Kong, Wei; Shan, Yaming

2016-05-25

The ectodomain of the influenza A virus (IAV) hemagglutinin (HA) stem is highly conserved across strains and has shown promise as a universal influenza vaccine in a mouse model. In this study, potential B-cell epitopes were found through sequence alignment and epitope prediction in a stem fragment, HA2:90-105, which is highly conserved among virus subtypes H1, H3 and B. A norovirus (NoV) P particle platform was used to express the HA2:90-105 sequences from subtypes H1, H3 and B in loops 1, 2 and 3 of the protrusion (P) domain, respectively. Through mouse immunization and microneutralization assays, the immunogenicity and protective efficacy of the chimeric NoV P particle (trivalent HA2-PP) were tested against infection with three subtypes (H1N1, H3N2 and B) of IAV in Madin-Darby canine kidney cells. The protective efficacy of the trivalent HA2-PP was also evaluated preliminarily in vivo by virus challenge in the mouse model. The trivalent HA2-PP immunogen induced significant IgG antibody responses, which could be enhanced by a virus booster vaccination. Moreover, the trivalent HA2-PP immunogen also demonstrated in vitro neutralization of the H3 and B viruses, and in vivo protection against the H3 virus. Our results support the notion that a broadly protective vaccine approach using an HA2-based NoV P particle platform can provide cross-protection against challenge viruses of different IAV subtypes. The efficacy of the immunogen should be further enhanced for practicality, and a better understanding of the protective immune mechanism will be critical for the development of HA2-based multivalent vaccines.

Wikis y aprendizaje colaborativo: lecciones aprendidas (y por aprender en la facultad de educación

Directory of Open Access Journals (Sweden)

Rocío Anguita Martínez

2010-01-01

Full Text Available El Espacio Europeo de Educación Superior ha llevado a las Universidades de toda Europa, entre ellas las españolas, a cambios metodológicos para su adaptación al Proceso de Bolonia. Estos entornos educativos suponen una nueva conceptualización de la docencia, en la que las Nuevas Tecnologías de apoyo a los procesos de enseñanza-aprendizaje adquieren un papel fundamental. En este escenario, una comunidad de profesores procedentes de la Facultad de Educación y Trabajo Social de la Universidad de Valladolid (UVa, vienen desarrollando desde hace años diversas innovaciones educativas en la asignatura de Nuevas Tecnologías Aplicadas a la Educación (NNTT. Innovaciones que han desencadenado en la puesta en práctica durante el curso académico 2007-2008 de una plataforma Wiki como eje transversal para el desarrollo de aprendizaje colaborativo. Este artículo analiza cómo desde una asignatura soportada por tecnología Wiki y orientada sobre los principios del aprendizaje por indagación (IBL se puede ayudar a promover el trabajo colaborativo y el pensamiento crítico entre los estudiantes desde la apertura de posibilidades didácticas y docentes.

Tendencias de la Educación universitaria en el siglo XXI

Directory of Open Access Journals (Sweden)

Ortega Castro, Vicente

2002-09-01

Full Text Available Not available

El cambio de milenio ha propiciado muchos informes, libros y conferencias sobre la situación de las Universidades y los posibles cambios que se avecinaban como consecuencia de las tendencias de cambios en la sociedad, entre los que se encuentran el predominio del conocimiento, laglobalización y la influencia de nuevas tecnologías aplicadas al ámbito de la educación. Para la sociedad europea, en la que España está inmersa política, social y económicamente, es especialmente relevante la expectativa que está despertando la Declaración de Bolonia. Pues bien, tomando este documento como referencia principal, este artículo examina las funciones permanentes de la Universidad: creación de conocimientos, formación de profesionales, servicio a la sociedad y otros, teniendo en cuenta la importancia de la calidad, de la internacionalización, de la competitividad, de la movilidad, de la complejidad, de las nuevas demandas, de los nuevos esquemas de financiación y otros factores que hacen necesario que las Universidades comprendan las nuevas tendencia y hagan un esfuerzo, no solo de adaptación, sino de anticipación.

APRENDIZAJE BASADO EN PROBLEMAS (ABP: UNA EXPERIENCIA PEDAGOGICA EN MEDICINA

Directory of Open Access Journals (Sweden)

Claudio Lermanda S.

2007-01-01

Full Text Available La enseñanza tradicional de la Medicina ha sido cuestionada por el avance tecnológico y el exponencial aumento de información médica accesible, obligando a reformular los curricula médicos bajo la mirada de las reformas educacionales que a escala global vienen desarrollándose desde el Proceso de Bolonia. Una propuesta curricular nueva señalada por Harden (1984 y conocida como modelo SPICES permite incorporar una metodología didáctica más apropiada para el proceso de enseñanza - aprendizaje formativo e integrador que demanda la sociedad actual. En este nuevo escenario, muchas Facultades de Medicina han incorporado el Aprendizaje Basado en Problemas (ABP como herramienta de aproximación al modelo señalado, por su carácter integrador de conocimientos, destrezas y actitudes que facilitan la adquisición de las competencias clínicas necesarias para el ejercicio profesional futuro. Este trabajo resume las consideraciones y sugerencias relativas al modelo y a la metodología tras una experiencia de dos años de ABP en la Facultad de Medicina de la Universidad Católica de la Santísima Concepción.

Tensile behaviour and properties of a bone analogue composite (HA, HDPE) crosslinked by gamma radiation

International Nuclear Information System (INIS)

Romero, G.; Smolko, Eduardo E.

2005-01-01

A natural composite material, hydroxyapatite (HA) and high density polyethylene (HDPE) crosslinked by ionizing radiations is been developed as a bioactive analogue material for bone replacement. Mechanical properties of the composites irradiated up to 300 kGy under tensile tests was studied. Gel content and micrographs of different composite fractures are shown. (author)

Provisioning the Ritual Neolithic Site of Kfar HaHoresh, Israel at the Dawn of Animal Management.

Science.gov (United States)

Meier, Jacqueline S; Goring-Morris, A Nigel; Munro, Natalie D

2016-01-01

It is widely agreed that a pivotal shift from wild animal hunting to herd animal management, at least of goats, began in the southern Levant by the Middle Pre-Pottery Neolithic B period (10,000-9,500 cal. BP) when evidence of ritual activities flourished in the region. As our knowledge of this critical change grows, sites that represent different functions and multiple time periods are needed to refine the timing, pace and character of changing human-animal relationships within the geographically variable southern Levant. In particular, we investigate how a ritual site was provisioned with animals at the time when herd management first began in the region. We utilize fauna from the 2010-2012 excavations at the mortuary site of Kfar HaHoresh-the longest continuous Pre-Pottery Neolithic B faunal sequence in the south Levantine Mediterranean Hills (Early-Late periods, 10,600-8,700 cal. BP). We investigate the trade-off between wild and domestic progenitor taxa and classic demographic indicators of management to detect changes in hunted animal selection and control over herd animal movement and reproduction. We find that ungulate selection at Kfar HaHoresh differs from neighboring sites, although changes in dietary breadth, herd demographics and body-size data fit the regional pattern of emerging management. Notably, wild ungulates including aurochs and gazelle are preferentially selected to provision Kfar HaHoresh in the PPNB, despite evidence that goat management was underway in the Mediterranean Hills. The preference for wild animals at this important site likely reflects their symbolic significance in ritual and mortuary practice.

CRISIS Y CRÉDITOS / Crisis and credits

Directory of Open Access Journals (Sweden)

Juan Luis Trillo de Leya

2010-05-01

Full Text Available RESUMEN El deseo de hacer más objetivos los criterios de valoración, tanto en la docencia como en la investigación, han introducido términos económicos en el vocabulario universitario cuando asistimos al peor momento de una crisis económica global. La unificación de los criterios se ha producido a la baja, nos ha llevado a valorar más la cantidad que la calidad y la pasividad más que la actividad. En esta situación nos enfrentamos a la reforma de los planes de estudio conforme a las directivas de la reunión de Bolonia, reforma que debemos aprovechar para reconsiderar y mejorar la situación actual. El debate se produce en torno a los significados de los términos: transversalidad, coordinación, integración, compactación, esponjamiento, actividad, simultaneidad y secuencia. SUMMARY The desire to make evaluation criteria more objective, in both teaching and research, has introduced economic terms into the university vocabulary when we are in the middle of a global, economic crisis. The unification of the criteria has produced a downturn and has led us to value quantity more than quality and passivity more than activity. In this situation we approach the reform of the curricula according to the directives of the meeting in Bologna, a reform which we must use to reassess and to improve the present situation. The debate takes place around the meaning of the terms: transversality, coordination, integration, compaction, absorption, activity, simultaneousness and sequence.

La educación superior a distancia y el e-Learning en las prisiones en Portugal

Directory of Open Access Journals (Sweden)

2017-04-01

Full Text Available La enseñanza superior en Europa ha venido experimentando grandes transformaciones gracias a los avances tecnológicos y a los cambios derivados del proceso de Bolonia, que prevé la creación de condiciones para que todos tengan acceso al aprendizaje, independientemente de factores de exclusión social, como la reclusión. La subsiguiente masificación del acceso a la Enseñanza Superior exige una oferta educativa flexible, centrada en el estudiante e inclusiva: son estas las prioridades de la Educación a Distancia de la Universidade Aberta en Portugal. Este artículo tiene como objetivo conocer las impresiones de veintiséis individuos (estudiantes, candidatos a estudiantes y técnicos superiores de reeducación sobre la realidad de la Enseñanza Superior en la modalidad de Educación a Distancia y aprendizaje virtual (e-Learning en una cárcel portuguesa. Este estudio se encuadra en un paradigma no positivista y hace hincapié en la percepción de los individuos, recurriendo a una metodología de carácter cualitativo. Los resultados, obtenidos mediante entrevistas, revelan la existencia de enormes debilidades y limitaciones en el proceso educativo, debido, sobre todo, a la falta de instalaciones y medios, recursos pedagógicos y tecnológicos, y apoyo de los profesores. Se ha concluido que es fundamental crear mejores condiciones de asistencia a distancia en las cárceles, porque esta formación superior puede ser una oportunidad de cualificación profesional y desarrollo personal, que incrementa sus perspectivas de éxito en el futuro.

El escenario de la calidad en la Universidad española: de dónde venimos y hacia dónde vamos

Directory of Open Access Journals (Sweden)

Jesús Miguel Muñoz-Cantero

2014-01-01

Full Text Available Durante la década de los 90 del pasado siglo, toman relevancia en la educación universitaria española conceptos y visiones hasta entonces desconocidos: sistema de indicadores, evaluación de programas/títulos, aseguramiento de la calidad, homologación, acreditación...La escasa permeabilidad del sistema no posibilitó la impregnación de la cultura de la calidad en todos sus estratos o niveles y en todas las Unidades. La Declaración de Bolonia, en junio de 1999, fue el impulso decisivo para la construcción del Espacio Europeo de Educación Superior (EEES en España. Desde entonces se ha diseñado la moderna arquitectura normativa para mejorar su calidad docente investigadora y de gestión. La Ordenación de las Enseñanzas Universitarias Oficiales recoge la necesidad de establecer criterios que faciliten la evaluación, certificación y acreditación. El establecimiento de un Sistema de Garantía de Calidad (SGC es de obligado cumplimiento para el proceso de Verificación y Acreditación de los Títulos. Iniciada la implantación de las enseñanzas correspondientes, los órganos de evaluación que la ley ha establecido, son los encargados de llevar a cabo el seguimiento partiendo del SGC adoptado y que orienta la futura acreditación de los títulos. Hoy por hoy, el protocolo de renovación de la acreditación de títulos está aún por dilucidar de manera definitiva.

Biomolecule-loaded chitosan nanoparticles induce apoptosis and molecular changes in cancer cell line (SiHa).

Science.gov (United States)

Sujima Anbu, Anbu; Velmurugan, Palanivel; Lee, Jeong-Ho; Oh, Byung-Taek; Venkatachalam, Perumal

2016-07-01

The present study reports on the synthesis of chitosan nanoparticles (CNPs) using methanol extracts of Gymnema sylvestre (GS) leaves and Cinnamomum zeylanicum (CZ) bark. Biomolecule-loaded nanoparticles induced apoptosis in a human cervical cancer (SiHa) cell line, and experiments were carried out to elucidate the underlying molecular mechanisms. FT-IR and XRD showed possible functional groups of the biomolecules and the crystalline nature of CNPs, respectively. Transmission electron microscopy images revealed that synthesized GSCNPs and CZCNPs had a smooth spherical shape with average sizes of about 58-80 and 60-120nm, respectively. Dynamic light scattering studies indicated that both GSCNPs and CZCNs were structurally stable with homogenous and heterogeneous natures, respectively. Furthermore, synthesized GSCNPs and CZCNPs exhibited dose-dependent cytotoxicity against the SiHa cancer cell line, with inhibitory concentration (IC50) values of 102.17μg/ml, 87.75μg/ml, 132.74μg/ml and 90.35μg/ml for GS leaf extract, GSCNPs, CZBE and CZCNPs, respectively. Copyright © 2016 Elsevier B.V. All rights reserved.

Sr, Mg cosubstituted HA porous macro-granules: potentialities as resorbable bone filler with antiosteoporotic functions.