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Sample records for guillaume wack asn

  1. Summary of the report Asn n. 2006 E.N.S.T.R. 019 - I.G.A.S. n. R.M. 2007-015 P. on the radiotherapy accident occurring at Epinal, presented by Guillaume WACK (Asn) and the doctor Francoise LALANDE, member of the General Inspection of social affairs, with the help of Marc David SELIGMAN; Resume du rapport ASN n. 2006 ENSTR 019 - IGAS n. RM 2007-015P sur l'accident de radiotherapie d'Epinal, presente par Guillaume WACK (ASN), et le docteur Francoise LALANDE, membre de l'Inspection Generale des affaires sociales, avec le concours de Marc David SELIGMAN

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-02-15

    In may 2004, the protocol of conformal radiotherapy applied to the prostate tumors has been modified. The change concerned the use of dynamic wedges in place of static wedges. This change supposed to change also the parameters of the calculation of irradiation intensity but this will not be made for some patients. This error could be corrected if the in vivo dosimetry had been kept and if the calculation of the number of monitors units had been maintained. 23 patients were submitted to an overexposure between the 6. may 2004 and the 1. august 2005. The first symptoms appeared in january 2005. The patients consulted their personal practitioner who sent them to a gastro-enterologist. The endoscopy revealed the existence of a rectitis which went by aggravating. In August, 2005 they were ten patients with severe hurts. During summer 2005, a radiotherapist doctor and a radio physicist found the error of overdose, They warned the manager of the hospital who warned in her turn, the D.D.A.S.S. (department direction of sanitary and social affairs) and A.R.H. (regional authority of hospitalization). Only seven patients were informed of the overdose, the sixteen other ones supposed wrongly unhurt, were not informed about the accident. The national authorities (I.R.S.N.) were warned in july 2006. Between september 2005 and september 2006 four patients died. Ten patients had severe damages, nine a moderate one. The report gives some propositions to avoid these kind of accidents in the future. (N.C.)

  2. Summary of the report Asn n. 2006 E.N.S.T.R. 019 - I.G.A.S. n. R.M. 2007-015 P. on the radiotherapy accident occurring at Epinal, presented by Guillaume WACK (Asn) and the doctor Francoise LALANDE, member of the General Inspection of social affairs, with the help of Marc David SELIGMAN

    International Nuclear Information System (INIS)

    2007-02-01

    In may 2004, the protocol of conformal radiotherapy applied to the prostate tumors has been modified. The change concerned the use of dynamic wedges in place of static wedges. This change supposed to change also the parameters of the calculation of irradiation intensity but this will not be made for some patients. This error could be corrected if the in vivo dosimetry had been kept and if the calculation of the number of monitors units had been maintained. 23 patients were submitted to an overexposure between the 6. may 2004 and the 1. august 2005. The first symptoms appeared in january 2005. The patients consulted their personal practitioner who sent them to a gastro-enterologist. The endoscopy revealed the existence of a rectitis which went by aggravating. In August, 2005 they were ten patients with severe hurts. During summer 2005, a radiotherapist doctor and a radio physicist found the error of overdose, They warned the manager of the hospital who warned in her turn, the D.D.A.S.S. (department direction of sanitary and social affairs) and A.R.H. (regional authority of hospitalization). Only seven patients were informed of the overdose, the sixteen other ones supposed wrongly unhurt, were not informed about the accident. The national authorities (I.R.S.N.) were warned in july 2006. Between september 2005 and september 2006 four patients died. Ten patients had severe damages, nine a moderate one. The report gives some propositions to avoid these kind of accidents in the future. (N.C.)

  3. Saussure, Guillaume, Hjelmslev

    OpenAIRE

    Blanchaud, Pierre

    2013-01-01

    Le concept saussurien de synchronie fait ici l’objet d’un examen détaillé à partir des critiques convergentes de plusieurs linguistes. Sont ensuite précisés deux autres concepts : l’acte de langage et l’effection. L’article se termine par une comparaison des linguistiques de Guillaume et de Hjelmslev : tandis que le premier chercheur procède de manière inductive et explicative, la méthode du second est déductive et descriptive. The Saussurian concept of synchrony is here subject of a detai...

  4. ASN reputation system model

    Science.gov (United States)

    Hutchinson, Steve; Erbacher, Robert F.

    2015-05-01

    Network security monitoring is currently challenged by its reliance on human analysts and the inability for tools to generate indications and warnings for previously unknown attacks. We propose a reputation system based on IP address set membership within the Autonomous System Number (ASN) system. Essentially, a metric generated based on the historic behavior, or misbehavior, of nodes within a given ASN can be used to predict future behavior and provide a mechanism to locate network activity requiring inspection. This will provide reinforcement of notifications and warnings and lead to inspection for ASNs known to be problematic even if initial inspection leads to interpretation of the event as innocuous. We developed proof of concept capabilities to generate the IP address to ASN set membership and analyze the impact of the results. These results clearly show that while some ASNs are one-offs with individual or small numbers of misbehaving IP addresses, there are definitive ASNs with a history of long term and wide spread misbehaving IP addresses. These ASNs with long histories are what we are especially interested in and will provide an additional correlation metric for the human analyst and lead to new tools to aid remediation of these IP address blocks.

  5. ASN annual report 2004

    International Nuclear Information System (INIS)

    2005-01-01

    The Nuclear Safety Authority (ASN) groups the Directorate General for Nuclear Safety and Radiation Protection (DGSNR), reporting to the Ministries for Health, Environment and Industry, and the devolved services of the State on which it relies for nuclear safety and radiation protection supervision, primarily DRIRE, DRASS and DDASS. The main topic of 2004, discussed in this report are: nuclear Safety and Transparency bill, shaping the new ASN, setting up inspection of non-BNI nuclear activities, Radiation protection and monitoring of patients, the safety of the EPR reactor project, the national plan for radioactive waste management, the future of high-level long-lived waste, harmonizing nuclear safety in Europe and ASN international involvement. (A.L.B.)

  6. Freaks Freearchitects / Yves Pasquet, Cyril Gauthier, Guillaume Aubry ; intervjueerinud Eve Arpo

    Index Scriptorium Estoniae

    Pasquet, Yves

    2016-01-01

    Prantsuse arhitektuuribüroost Freaks Freearchitects. Büroo arhitektid Yves Pasquet, Cyril Gauthier ja Guillaume Aubry tegelevad arhitekti rolli määratlemisega installatsioonide, filmi ning arhitektuursete projektide kaudu

  7. External PCR, ASN's decision

    International Nuclear Information System (INIS)

    Anon.

    2012-01-01

    The French law imposes in some situations the presence of a person skilled in radiation protection (PCR). This article describes the cases when this person must belong to the staff of the enterprise or when this person may be sub-contracted. For instance in most nuclear facilities the PCR must be on the payroll, for enterprises dedicated to nuclear transport the PCR's job can be sub-contracted. A decision given by the ASN (French Nuclear Safety Authority) sets the minimal requests (in terms of training, job contract, activities) of the sub-contracted PCR. (A.C.)

  8. ASN 2009 annual report

    International Nuclear Information System (INIS)

    2010-01-01

    After a presentation of the roles, missions and organization of the ASN (the French Nuclear safety Authority), this document presents and reports its actions in various domains: ionizing radiations and health and environmental risks (knowledge on these risks and hazards, nuclear activities controlled by the ASN, surveillance of exposures to ionizing radiations), principles and actors of nuclear safety, radioprotection and environment protection control, regulation (general framework, proximity nuclear regulation, nuclear installation legal regime, radioactive material transport regulation, arrangements associated with some risks and some specific activities), control of nuclear activities and exposures to ionizing radiations, situations of radiological emergency, public information, international relationships (objectives in Europe and in the world, relationships within the EU and multilateral relationships, bilateral relationships, international convention, international conferences), regional overview of nuclear safety and radioprotection, medical and non medical uses of ionizing radiations, transport of radioactive materials, EDF nuclear plants, nuclear fuel cycle installations, nuclear research installations and other installations, basic nuclear installation dismantling safety, radioactive wastes and polluted sites. This report also provides presentations of incident and accident classification scales and law texts

  9. The Poetics of Visual Cubism: Guillaume Apollinaire on Pablo Picasso

    Directory of Open Access Journals (Sweden)

    Pamela A. Genova

    2003-01-01

    Full Text Available Guillaume Apollinaire, one of the most original poets of the early twentieth-century French avant garde , played a crucial role in the enunciation of modernist aesthetics. Through innovative poetic forms, Apollinaire set forth a new aesthetics which underscored the inherent ambiguity of an increasingly turbulent modern context. Apollinaire's interest in the pure dynamism of the contemporary material landscape, and his attraction to the image that explodes with immediate presence, also led him to a natural curiosity in the visual arts. Identifying with the Cubist mosaic style of inclusion, the juxtaposition of reality and imagination, and the simultaneity of spatial and temporal movement, Apollinaire saw modern artists as "singers of a constantly new truth," inventors of a uniquely authentic modern experience. Apollinaire composed verse to honor his favorite painters, but he also wrote critical studies on the visual arts, and he declares that it is in Cubist art that we can discover a truly successful endeavor to come to terms with the upheavals of modernity. In several texts Apollinaire devotes specifically to Picasso, he argues that his canvases contain the most essential aspects of modern art: a new interpretation of light, a genuine understanding of the elusive notion of the "fourth dimension," and an incarnation of the most modern of principles, surprise. Apollinaire's texts on Picasso, examples of his poésie critique , do not remain simply words printed on a page, but are transformed into an extension of the painting he wishes to convey, experimental and unpredictable in discursive tone and poetic style. Through these texts, Apollinaire moves beyond the parameters of a journalistic style of criticism, as his pieces on Picasso take on a chameleon-like power of movement, engendering unique forms of an avant-garde improvisation, the painting of prose poetry.

  10. ASN annual report 2004; ASN Rapport annuel 2004

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2005-07-01

    The Nuclear Safety Authority (ASN) groups the Directorate General for Nuclear Safety and Radiation Protection (DGSNR), reporting to the Ministries for Health, Environment and Industry, and the devolved services of the State on which it relies for nuclear safety and radiation protection supervision, primarily DRIRE, DRASS and DDASS. The main topic of 2004, discussed in this report are: nuclear Safety and Transparency bill, shaping the new ASN, setting up inspection of non-BNI nuclear activities, Radiation protection and monitoring of patients, the safety of the EPR reactor project, the national plan for radioactive waste management, the future of high-level long-lived waste, harmonizing nuclear safety in Europe and ASN international involvement. (A.L.B.)

  11. Una versió valenciana quatrecentista desconeguda de la «Obsidionis Rhodie» de Guillaume Caoursin

    Directory of Open Access Journals (Sweden)

    Albert G. Hauf i Valls

    2015-10-01

    Full Text Available This is a reconstruction of the only extant Catalan versión of Guillaume Caoursin's Obsidionis Rhodie descriptio, based on the passages of this interesting text assimiled by Joan Esteve in his Liber elegantiarum.

  12. The French Nuclear Safety Authority - ASN

    International Nuclear Information System (INIS)

    2013-01-01

    The ASN (Nuclear Safety Authority) was created by the act of 13 June 2006 concerning the transparency and safety of nuclear activities. The ASN is an independent administrative body that is in charge of controlling nuclear activities in France. The ASN has a workforce of 471 people and a budget of about 76 millions euros. This article details its missions and how it is organized to cover all the French territory. (A.C.)

  13. Energy efficiency at the St-Guillaume cheese factory, a profitable choice; L'efficacite energetique a la fromagerie St-Guillaume, un choix profitable

    Energy Technology Data Exchange (ETDEWEB)

    Tremblay, P.; La Roche, R. [Fromagerie St-Guillaume, Saint-Guillaume, PQ (Canada)

    2009-12-15

    The Co-op Agrilait de St-Guillaume is located at near Drummondville, Quebec. The facility employs 160 people to produce 3 million kilograms of cheese annually. In addition, 21 million litres of whey are produced. This sub-product of cheese is turned into powder to make ice cream, protein shakes, energy drinks, and feed for animals. The cost of transforming whey had gone up over the last five years, which also cut into the operating budget. Whey transformation also consumed large amounts of well water. In order to address these challenges, the energy consumption of the facility was evaluated in an effort to lower the energy costs by 30 per cent, lower water consumption and reduce water pollution. The study identified and measured the energy consumption for each activity related to the whey transformation process. The equipment and procedures used in the facility were subsequently modernized and the air quality in the facility was also improved. The improvement in whey transformation rendered the facility profitable again. The project was financed by capital loans over a ten year period, and the money saved during the first year was over $400,000. The money saved over the next 10 years will be more than 2 million dollars. 5 figs.

  14. The French Nuclear Safety Authority (ASN)

    International Nuclear Information System (INIS)

    Alloso, Ph.

    2011-01-01

    This article presents the statutes, the organization and the missions of the Nuclear Safety Authority (ASN) whose scope includes radiation protection since 2002. Globally ASN is in charge of: -) participating to the making of laws and regulations, -) delivering administrative authorizations, -) controlling the conformity of nuclear installations and activities with the laws and regulations, -) informing the public, and -) reporting on the state of nuclear safety and radiation protection each year. (A.C.)

  15. Reflection groups established by the ASN

    International Nuclear Information System (INIS)

    Fournier, M.

    2009-01-01

    Recent observations about tritium bio-kinetics in aquatic organisms might change our usual consideration about its radio-toxicity. In order to analyse these observations more thoroughly, the French nuclear safety authority (ASN) decided at the end of the year 2007 to create two independent reflection groups gathering scientists, nuclear operators and associations: the group 'tritium impact' has in charge the establishment of a status of the scientific knowledge relative to tritium sanitary impact, while the group 'defense in depth' has in charge the examination of the technical possibilities of tritium reprocessing and the establishment of a knowledge status of its environmental impact. First meetings of both groups took place in May 2008. Abstract only. (J.S.)

  16. Asn12 and Asn278: Critical Residues for In Vitro Biological Activity of Reteplase

    Directory of Open Access Journals (Sweden)

    Naganath Mandi

    2010-01-01

    Full Text Available Reteplase (rPA is a thrombolytic agent used for the treatment of acute myocardial infarction. We studied the expression of rPA and its selected asparagine mutants after integration into the Pichia genome. Though methanol induction of the native and the rPA mutants showed similar expression levels (~200–250 mg/L, the mutants displayed significant loss of protease activity. Strikingly, the clot lysis activities of these mutants were considerably different. While mutation of Asn12 (N12P of the Kringle 2 domain showed delayed clot lysis activity (1/2=38 min compared to the native rPA (1/2=33 min, a faster rate of clot lysis (1/2=27 min was observed when the Asn278 (N278S of the serine protease domain was mutated. Interestingly, the slowest clot lysis activity (1/2=49 min demonstrated by the double mutant (N12P, N278S suggests the dominant role of Asn12 in regulating the fibrinolytic activity of rPA. The results presented in this paper indicate that the fibrinolytic and the proteolytic activities of rPA are independent of each other.

  17. Skills of Asn inspectors responsible for inspecting pressurised equipment

    International Nuclear Information System (INIS)

    Colonna, F.

    2010-01-01

    ASN relies on the unique competencies of its staff to assure that it can complete its mission of maintaining public health and safety. Since 1975, personnel at ASN Nuclear Pressure Equipment Department have played a major role in the oversight of pressure boundary equipment by verifying that equipment is manufactured in compliance with regulatory requirements. This has called for highly competent individuals trained in a variety of technical and regulatory backgrounds. ASN is meeting the challenge of keeping its staff trained and prepared to continue its oversight of nuclear boilers. This has included ensuring that its organization is welt suited to meet the demands, and relying on third-party experts when necessary. With the implementation of the 2006 transparency law, ASN has taken necessary steps to ensure that it will maintain its high level of expertise going forward. (author)

  18. Of two lives one? Jean-Charles-Marguerite-Guillaume Grimaud and the question of holism in vitalist medicine.

    Science.gov (United States)

    Williams, Elizabeth A

    2008-12-01

    Montpellier vitalists upheld a medical perspective akin to modern "holism" in positing the functional unity of creatures imbued with life. While early vitalists focused on the human organism, Jean-Charles-Marguerite-Guillaume Grimaud investigated digestion, growth, and other physiological processes that human beings shared with simpler organisms. Eschewing modern investigative methods, Grimaud promoted a medically-grounded "metaphysics." His influential doctrine of the "two lives" broke with Montpellier holism, classifying some vital phenomena as "higher" and others as "lower" and attributing the "nobility" of the human species to the predominance of the former. In place of Montpellier teaching that attributed health to the holistic equilibration of vital activities, Grimaud embraced spiritualist dualisms of soul and body, Creator and created. Celebrating the divinely-ordained "wisdom" evident in involuntary physiological processes, he argued that such life functions were incomprehensible to human investigators. While Grimaud's work encouraged inquiry into the division between the central and "vegetative" nervous systems that became paradigmatic in nineteenth-century neuroscience, it also opened Montpellier vitalism to charges of conservatism and obscurantism that are still lodged against it to the present day.

  19. ASN annual report 2007 - ASN report abstracts on the state of Nuclear Safety and Radiation Protection in France in 2007; ASN rapport annuel 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    The 2007 annual report of the French nuclear safety authority (ASN) presents the highlights of the year in the domain of nuclear safety and radiation protection in France. The year 2007 was marked by the implementation of a new legislative and regulatory framework created by the 13 June 2006 Act on transparency and security in the nuclear field (TSN) and the 28 June Act on the sustainable management of radioactive materials and wastes. As in the previous two years, the year was relatively satisfactory from the nuclear safety viewpoint and slightly less so with regard to small-scale nuclear activities. For two years now, the medical field has been marked by the declaration to ASN of a number of serious radiotherapy accidents which have led to several deaths or the need for extensive surgery. The following main topics are reviewed in the document: 1 - the decrees implementing the 'TSN' act and the 'Waste' act; 2 - the new ASN, one year on; 3 - ASN regulatory actions in the field of radiotherapy; 4 - regulation of new installations; 5 - the key issues for regulation of existing installations; 6 - nuclear safety and radiation protection research; 7 - policy for management of the post-accident phase of a radiological emergency; 8 - sites and soils polluted by radioactive materials; 9 - international harmonization of nuclear safety and radiation protection.

  20. ASN annual report 2007 - ASN report abstracts on the state of Nuclear Safety and Radiation Protection in France in 2007; ASN rapport annuel 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2008-07-01

    The 2007 annual report of the French nuclear safety authority (ASN) presents the highlights of the year in the domain of nuclear safety and radiation protection in France. The year 2007 was marked by the implementation of a new legislative and regulatory framework created by the 13 June 2006 Act on transparency and security in the nuclear field (TSN) and the 28 June Act on the sustainable management of radioactive materials and wastes. As in the previous two years, the year was relatively satisfactory from the nuclear safety viewpoint and slightly less so with regard to small-scale nuclear activities. For two years now, the medical field has been marked by the declaration to ASN of a number of serious radiotherapy accidents which have led to several deaths or the need for extensive surgery. The following main topics are reviewed in the document: 1 - the decrees implementing the 'TSN' act and the 'Waste' act; 2 - the new ASN, one year on; 3 - ASN regulatory actions in the field of radiotherapy; 4 - regulation of new installations; 5 - the key issues for regulation of existing installations; 6 - nuclear safety and radiation protection research; 7 - policy for management of the post-accident phase of a radiological emergency; 8 - sites and soils polluted by radioactive materials; 9 - international harmonization of nuclear safety and radiation protection.

  1. ASN report abstracts on the state of Nuclear Safety and Radiation Protection in France in 2008. ASN annual report 2008

    International Nuclear Information System (INIS)

    2009-01-01

    ASN is an independent administrative authority tasked, on behalf of the state, with regulating nuclear safety and radiation protection in order to protect workers, patients and the environment from the hazards involved in nuclear activities. It also contributes to informing the public. ASN, run by a five-member commission, regulates the whole of the sector, acting in a completely impartial manner. The ASN Commission presents its report on the state of nuclear safety and radiation protection in France in 2008. Contents: A - Introduction: The year 2008. B - Key topics: 1. ASN actions to promote greater transparency; 2. EDF nuclear power plant ageing and operating life: the conditions for continued operation; 3. The prevention of malicious acts; 4. ASN regulation of the radiotherapy sector; 5. Regulating and monitoring the construction of the Flamanville 3 EPR reactor; 6. ASN's international nuclear safety responsibilities; 7. The new nuclear countries; 8. Decommissioning of basic nuclear installations and the low-level, long-lived waste disposal project. C - Overview: 1 - Nuclear activities: ionising radiations and health risks; 2 - Principles and stakeholders in the regulation of nuclear safety and radiation protection; 3 - Regulation; 4 - Regulation of nuclear activities and exposure to ionising radiations; 5 - Environmental protection; 6 - Public information and transparency; 7 - International relations; 8 - Radiological emergencies; 9 - Medical uses of ionising radiations; 10 - Industrial and research activities; 11 - Transport of radioactive materials; 12 - EDF nuclear power plants; 13 - Nuclear fuel cycle installations; 14 - Nuclear research facilities and various nuclear installations; 15 - Safe decommissioning of basic nuclear installations; 16 - Radioactive waste and polluted sites. D - Appendices: List of basic nuclear installations; ASN decisions and opinions published in 2008 in its Official Bulletin; Acronyms, abbreviations and names

  2. A.S.N. regulation of Flamanville 3 EPR construction

    International Nuclear Information System (INIS)

    Limousin, S.

    2009-01-01

    The EPR authorization decree for Flamanville 3 was issued by the French government (decree no. 2007-534) on the 10 April 2007. The construction activities are going on in the manufacturer workshops and at Flamanville site. The decree published in November 2007, and related to the so called TSN law (the nuclear transparency and safety nuclear law published in 2006), specifies that the commissioning licence (the agreement for first fuel load) has to be granted by an ASN decision on the basis of the assessment of the demonstration of the plant safety. In this aim, one part of ASN's work is to review, with its technical sup-port organisation (IRSN), the reactor construction in order to appreciate the safety level of the construction activities. This paper describes ASN's strategy for the regulation of Flamanville 3 EPR construction activities, endorsed by ASN commission at the end of November 2007, and the main events which have occurred on Flamanville site since the beginning of the construction. The article presents the main ASN conclusions of this regulation at this time. (author)

  3. ASN annual report 2007 - ASN report abstracts on the state of Nuclear Safety and Radiation Protection in France in 2007

    International Nuclear Information System (INIS)

    2008-01-01

    The 2007 annual report of the French nuclear safety authority (ASN) presents the highlights of the year in the domain of nuclear safety and radiation protection in France. The year 2007 was marked by the implementation of a new legislative and regulatory framework created by the 13 June 2006 Act on transparency and security in the nuclear field (TSN) and the 28 June Act on the sustainable management of radioactive materials and wastes. As in the previous two years, the year was relatively satisfactory from the nuclear safety viewpoint and slightly less so with regard to small-scale nuclear activities. For two years now, the medical field has been marked by the declaration to ASN of a number of serious radiotherapy accidents which have led to several deaths or the need for extensive surgery. The following main topics are reviewed in the document: 1 - the decrees implementing the 'TSN' act and the 'Waste' act; 2 - the new ASN, one year on; 3 - ASN regulatory actions in the field of radiotherapy; 4 - regulation of new installations; 5 - the key issues for regulation of existing installations; 6 - nuclear safety and radiation protection research; 7 - policy for management of the post-accident phase of a radiological emergency; 8 - sites and soils polluted by radioactive materials; 9 - international harmonization of nuclear safety and radiation protection

  4. Neurological Impact of World War I on the Artistic Avant-Garde: The Examples of André Breton, Guillaume Apollinaire and Blaise Cendrars.

    Science.gov (United States)

    Bogousslavsky, Julien; Tatu, Laurent

    2016-01-01

    World War I erupted at a time when artistic avant-gardes were particularly thriving across Europe. Young poets, writers, painters and sculptors were called to arms or voluntary enrolled to fight, and several of them died during the conflict. Among others, it dramatically changed their creative output, either through specific wounds or through personal encounters and experiences. These individual events then significantly modified the course of the literary and artistic avant-garde movements. Three particularly illustrative examples of avant-garde French poets are presented here: André Breton (1896-1966), Guillaume Apollinaire (1880-1918) and Blaise Cendrars (1887-1961). The deep source of the surrealist movement can indeed be found in André Breton's involvement as an auxiliary physician with critical interest in neuropsychiatry, which caused him to discover automatic writing. Guillaume Apollinaire's right temporal subdural hematoma strongly modified his emotional state and subsequent artistic activities. Alternatively, after losing his right, writing hand, Blaise Cendrars not only substituted it with a phantom but also rapidly switched from poetry to novels after he learnt to write with his left hand. © 2016 S. Karger AG, Basel.

  5. Assessment of ASN inspections apart from BNI (Greater South West)

    International Nuclear Information System (INIS)

    Menechal, Ph.

    2008-01-01

    After having recalled recent evolutions of the ASN's organization (the French nuclear safety authority) and its basic missions (regulation elaboration, decisions, control of nuclear activities and installations, public information), the author presents the Bordeaux ASN's department which is in charge of the south-western part of France. He indicates and comments some data illustrating the performed actions and missions. He describes the objectives and procedures of inspection which may depend on the activity (external radiotherapy, interventional radiology, research laboratories). He assesses theses inspections in terms of radioprotection organization, of risk assessment and of definition of regulated areas, of personnel classification and dosimetric follow-on, of training of exposed personnel, of medical follow-on, of radioprotection control, of training of patients to radioprotection, of source management, and of waste and effluent management

  6. Accidental exposures in interventional radiology: lessons learned by the ASN

    International Nuclear Information System (INIS)

    Marchal, C.; Valero, M.

    2009-01-01

    The authors outline that interventional radiology often requires long duration exposures of patients to ionizing radiations and thus stress that interventional radiology must be optimized to improve radioprotection of patients and operators. They notice that investigations performed by the ASN (the French Nuclear Safety Authority) on declared events revealed in some cases a lack of knowledge of devices by users, notably of functionalities allowing the applied doses to be controlled

  7. The Bacillus subtilis and Bacillus halodurans Aspartyl-tRNA Synthetases Retain Recognition of tRNA(Asn).

    Science.gov (United States)

    Nair, Nilendra; Raff, Hannah; Islam, Mohammed Tarek; Feen, Melanie; Garofalo, Denise M; Sheppard, Kelly

    2016-02-13

    Synthesis of asparaginyl-tRNA (Asn-tRNA(Asn)) in bacteria can be formed either by directly ligating Asn to tRNA(Asn) using an asparaginyl-tRNA synthetase (AsnRS) or by synthesizing Asn on the tRNA. In the latter two-step indirect pathway, a non-discriminating aspartyl-tRNA synthetase (ND-AspRS) attaches Asp to tRNA(Asn) and the amidotransferase GatCAB transamidates the Asp to Asn on the tRNA. GatCAB can be similarly used for Gln-tRNA(Gln) formation. Most bacteria are predicted to use only one route for Asn-tRNA(Asn) formation. Given that Bacillus halodurans and Bacillus subtilis encode AsnRS for Asn-tRNA(Asn) formation and Asn synthetases to synthesize Asn and GatCAB for Gln-tRNA(Gln) synthesis, their AspRS enzymes were thought to be specific for tRNA(Asp). However, we demonstrate that the AspRSs are non-discriminating and can be used with GatCAB to synthesize Asn. The results explain why B. subtilis with its Asn synthetase genes knocked out is still an Asn prototroph. Our phylogenetic analysis suggests that this may be common among Firmicutes and 30% of all bacteria. In addition, the phylogeny revealed that discrimination toward tRNA(Asp) by AspRS has evolved independently multiple times. The retention of the indirect pathway in B. subtilis and B. halodurans likely reflects the ancient link between Asn biosynthesis and its use in translation that enabled Asn to be added to the genetic code. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  8. Impact of the Disruption of ASN3-Encoding Asparagine Synthetase on Arabidopsis Development

    Directory of Open Access Journals (Sweden)

    Laure Gaufichon

    2016-02-01

    Full Text Available The aim of this study was to investigate the role of ASN3-encoded asparagine synthetase (AS, EC 6.3.5.4 during vegetative growth, seed development and germination of Arabidopsis thaliana. Phenotypic analysis of knockout (asn3-1 and knockdown (asn3-2 T-DNA insertion mutants for the ASN3 gene (At5g10240 demonstrated wild-type contents of asparagine synthetase protein, chlorophyll and ammonium in green leaves at 35 days after sowing. In situ hybridization localized ASN3 mRNA to phloem companion cells of vasculature. Young siliques of the asn3-1 knockout line showed a decrease in asparagine but an increase in glutamate. The seeds of asn3-1 and asn3-2 displayed a wild-type nitrogen status expressed as total nitrogen content, indicating that the repression of ASN3 expression had only a limited effect on mature seeds. An analysis of amino acid labeling of seeds imbibed with (15N ammonium for 24 h revealed that asn3-1 seeds contained 20% less total asparagine while 15N-labeled asparagine ((2-15Nasparagine, (4-15Nasparagine and (2,4-15Nasparagine increased by 12% compared to wild-type seeds. The data indicate a fine regulation of asparagine synthesis and hydrolysis in Arabidopsis seeds.

  9. Structural insight into gene transcriptional regulation and effector binding by the Lrp/AsnC family

    NARCIS (Netherlands)

    Thaw, P.; Sedelnikova, S.E.; Muranova, T.; Wiese, S.; Ayora, S.; Alonso, J.C.; Brinkman, A.B.; Akerboom, A.P.; Oost, van der J.; Rafferty, J.B.

    2006-01-01

    The Lrp/AsnC family of transcriptional regulatory proteins is found in both archaea and bacteria. Members of the family influence cellular metabolism in both a global (Lrp) and specific (AsnC) manner, often in response to exogenous amino acid effectors. In the present study we have determined both

  10. O Tipográfico e o topográfico na tradução poética: a visilegibilidade do poema Voyage de Guillaume Apollinaire.

    Directory of Open Access Journals (Sweden)

    Álvaro Silveira Faleiros

    2005-01-01

    Full Text Available In this article, it is shouwn briefly the importance of the typographic marks in the poetic reading and, consequently, in the translation of poetic texts. This point of view is illustrated with the analysis of some forms in which the figurative põem Voyage by Guillaume Apollinaire was graphically displayed in two French editions and one translation in Spanish, and finally my translation is presented. Keywords: Apollinaire, caligrams, figurative poem, typography, spatiality.

  11. [Conformation analysis of the N-glycosylation site Asn-X-Thr/Ser in glycoproteins].

    Science.gov (United States)

    Avanov, A Ia; Lipkind, G M

    1990-03-01

    Theoretical conformational analysis of oligopeptides CH3CO-Asn-X-Thr-NHCH3 (X = Gly, Ala, Pro), modelling N-glycosylation site, and their glycosylated derivatives CH3CO-(GlcNAc beta 1-4GlcNAc beta 1) Asn-X-Thr-NHCH3 has been carried out. Active conformations of the site are found, corresponding to structural prerequisities of N-glycosylation: Asn residue's position in beta-turn and hydrogen bond formation between side chains of Asn and Thr/Ser residues. In this case the L conformation of the central residue X is most probable. Since Pro residue does not possess this conformation, sequences with X = Pro are not glycosylated. It is shown that glycosylation of the above-mentioned sites is accompanied by reorientation of the Asn residue's side chains.

  12. ASN report on nuclear safety and radioprotection status in France in 2010

    International Nuclear Information System (INIS)

    2011-01-01

    After a general overview of the activity of the ASN (the French nuclear safety authority), of its missions and organisation, this activity report indicates the main events of 2010 concerning the actions performed by the ASN and the activities controlled by the ASN. These both aspects are then detailed. The actions performed by the ASN are dealing with nuclear activities (ionizing radiations and risks for health and for the environment), principles and actors of nuclear safety control, radioprotection and environment protection, regulation, control of nuclear activities and exposures to ionizing radiations, emergency situations, public information, international relationship, regional overview of nuclear safety and radioprotection. The activities controlled by the ASN are the medical uses of ionizing radiations, their non medical uses, the transport of radioactive materials, electronuclear plants, installations related to nuclear fuel cycle, nuclear research installations and other nuclear installations, the safety of nuclear installation dismantling, radioactive wastes and polluted sites

  13. The decisions taken in 2012 by the ASN: additional requirements

    International Nuclear Information System (INIS)

    Lacoste, Andre-Claude; Comets, Marie-Pierre; Dumont, Jean-Jacques; Bourguignon, Michel; Jamet, Philippe

    2012-01-01

    After the publication of conclusions of the Additions Safety Assessments (ECS, evaluations complementaires de surete), this document gathers 32 decisions taken by the ASN in June 2012 which specify additional requirements for EDF nuclear power plants, AREVA installations, and some CEA reactors. These measures will lead to a significant strengthening of safety margins beyond their sizing. These decisions impose huge works to operators. They comprise the implementation of an organisation and the construction of premises resistant to a big event which would affect several installations. Other measures concern a deeper training of agents intervening in case of earthquake or severe accident, robust means of power supply for warehousing pools, efficient means to reduce accidental leakage of different compounds, the storing of fissile materials in an installation sufficiently strong to resist earthquakes, and improvements regarding the flood risk management and sodium fire risk management

  14. Reflection groups established by the ASN; Les groupes de reflexion mis en place par l'ASN

    Energy Technology Data Exchange (ETDEWEB)

    Fournier, M. [ASN, 75 - Paris (France)

    2009-07-01

    Recent observations about tritium bio-kinetics in aquatic organisms might change our usual consideration about its radio-toxicity. In order to analyse these observations more thoroughly, the French nuclear safety authority (ASN) decided at the end of the year 2007 to create two independent reflection groups gathering scientists, nuclear operators and associations: the group 'tritium impact' has in charge the establishment of a status of the scientific knowledge relative to tritium sanitary impact, while the group 'defense in depth' has in charge the examination of the technical possibilities of tritium reprocessing and the establishment of a knowledge status of its environmental impact. First meetings of both groups took place in May 2008. Abstract only. (J.S.)

  15. The Disputed Authorship of a Medieval Text: A New Solution to the Attribution Problem in the Case of Guillaume D’Angleterre

    Directory of Open Access Journals (Sweden)

    Maria Slautina

    2012-11-01

    Full Text Available This paper presents a case of disputed authorship concerning a literary medieval text, the Old French romance Guillaume d’Angleterre, and describes a new attempt to use the mathematical method of authorship attribution called the "method of pattern recognition”. After presenting an overview of previous approaches to the problem of the authorship of this text, I argue for the advantages of a statistical syntactic based method for authorship attribution of medieval texts. The method of pattern recognition consists in the identification of a domain of syntactic parameters and a measurement of the proximity or distance of texts as located in a multi-dimensional syntactic space. I find that the medieval text most likely belongs to Chrétien de Troyes, one of the most famous French authors of the twelfth century. I present for the first time an attempt to apply the method of pattern recognition to determine the authorship of a medieval text written in Old French.

  16. Effects of Asn318 and Asp87Asn318 mutations on signal transduction by the gonadotropin-releasing hormone receptor and receptor regulation.

    Science.gov (United States)

    Awara, W M; Guo, C H; Conn, P M

    1996-02-01

    GnRH receptor (GnRH-R) contains Asn87 and Asp318 instead of the more frequently observed Asp87 and Asn318 found in other G protein-coupled receptors. In the present study, site-directed mutagenesis was used to introduce Asn318 and Asp87Asn318 into GnRH-R. The effect on coupling and regulation of GnRH-R was studied by stable expression of wild and mutant mouse GnRH-R in the lactotropic GH3 cells; these normally release PRL in response to TRH stimulation. The responses to Buserelin (a metabolically stable GnRH analog) in three different cell lines, M1, N8, and ND1 (expressing wild-type, Asn318 mutant, and Asp87Asn318 mutant mouse GnRH-R, respectively) were compared with that observed in the previously characterized GGH3-1' cells, which stably express rat GnRH-R. The Asn318 and Asp87Asn318 mutations had no measurable effect on ligand binding, but abolished the initial down-regulation of receptor that was observed in M1 and GGH3-1' cells, suggesting that the normal location of Asn87 and Asp318 in GnRH-R is involved in the regulation of GnRH-R. In N8 and ND1 cells, Buserelin-stimulated inositol phosphate (IP) production was attenuated, but the release of both cAMP and PRL was stimulated in a dose- and time-dependent manner. These mutations apparently impaired the coupling between GnRH-R and G proteins involved in IP production, but not those involved in cAMP release. In M1 cells, Buserelin stimulation produced a significant increase in IP production, but neither cAMP nor PRL release was significantly stimulated. These findings are consistent with the previous suggestion that GnRH-stimulated PRL release is mediated by a cAMP second messenger system in transfected GGH3 cells.

  17. ASN report of the status of nuclear safety and radiation protection in France in 2012

    International Nuclear Information System (INIS)

    Chevet, Pierre-Franck; Delmestre, Alain; Bardet, Marie-Christine; Covard, Fabienne; Landrin, Sophie

    2013-01-01

    After a presentation of the French Nuclear Safety Authority (ASN), its missions, some key figures illustrating its activities and its organisation, this report proposes an overview of marking events and of actions undertaken by the ASN after the Fukushima accident. Then, the report proposes a detailed and commented overview of actions undertaken by the ASN in different fields and domains: nuclear activities, principles and actors of nuclear safety and radiation protection control, regulation, control of nuclear activities and of exposures to ionizing radiations, radiological and post-accidental emergency situations, public information and transparency, international relationships, regional overview of nuclear safety and radiation protection. The last part addresses activities controlled by the ASN: medical use of ionizing radiations, industrial, research and veterinary uses and source safety, transport of radioactive materials, electronuclear plants, installations related to nuclear fuel cycle, nuclear research and other nuclear installations, safety of dismantling of base nuclear installations, radioactive wastes and polluted sites

  18. ASN report on nuclear safety and radiation protection in France in 2006, excerpts

    International Nuclear Information System (INIS)

    Lacoste, A.C.

    2007-01-01

    The main topics for the Nuclear Safety Authority (ASN) in 2006 were the following ones. 1) the 2006-686 bill on transparency and security in the nuclear field. This law constitutes an overhaul of the legislative framework applicable to nuclear activities. It creates the Nuclear Safety Authority as an independent administrative authority and defines its tasks. It contains significant advances in terms of transparency. 2) The 2006-739 bill concerning sustainable management of radioactive materials and waste. This law clearly states that the disposal in deep geological formations is the reference solution for high-level, long-lived radioactive wastes. 3) The EPR reactor project safety in which ASN completed technical examination of the preliminary safety case. In November 2006, ASN produced to French Authorities a draft decree concerning the EPR project on the Flamanville site. 4) An international audit of ASN was performed in 2006 by IAEA in order to assess the extent to which the standards IAEA recommends are taken into account and implemented. 5) ASN has contributed to the harmonization of nuclear safety in Europe and at world scale through its contribution to the work of the WENRA association. 6) In 2005 and 2006 4 serious radiotherapy accidents were reported in France. Cancer radiotherapy is a fully justified practice that demands a vigilant supervision of ASN. 7) ASN has amplified its means for informing the public about nuclear safety and radiation protection. And 8) ASN considers that people and organizations are fundamental factors in safety and radiation protection and that significant progress is still to be made in taking into account these factors in nuclear activities. (A.C.)

  19. Structural investigations of the active-site mutant Asn156Ala of outer membrane phospholipase A: Function of the Asn-His interaction in the catalytic triad

    NARCIS (Netherlands)

    Snijder, H.J.; van Eerde, J.H.; Kalk, K.H.; Dekker, N.; Egmond, M.R.; Dijkstra, B.W.

    2010-01-01

    Outer membrane phospholipase A (OMPLA) from Escherichia coli is an integral-membrane enzyme with a unique His-Ser-Asn catalytic triad. In serine proteases and serine esterases usually an Asp occurs in the catalytic triad; its role has been the subject of much debate. Here the role of the uncharged

  20. Olivier Chanel et Guillaume Faburel (dir., 2010, L’Environnement dans la décision publique : Refonder l’évaluation socio-économique pour des politiques de transport plus durables, Economica, Paris, 230 p.

    Directory of Open Access Journals (Sweden)

    Adrien de Abreu

    2010-09-01

    Full Text Available Quid des impacts environnementaux dans l’évaluation socio-économique des projets d’infrastructure ? C’est sous l’angle des équipements de transport que les auteurs abordent cette question, dirigés par Olivier Chanel et Guillaume Faburel. Olivier Chanel est chercheur en économie de l’environnement au sein du GREQAM (Groupement de recherche en économie quantitative d’Aix-Marseille. Guillaume Faburel est Maître de conférences à l’Université Paris 12 - Val de Marne et chercheur au CRETEIL (Centr...

  1. N-Glycosylation analysis of yeast Carboxypeptidase Y reveals the ultimate removal of phosphate from glycans at Asn368.

    Science.gov (United States)

    B S, Gnanesh Kumar; Surolia, Avadhesha

    2017-05-01

    Carboxypeptidase Y from Saccharomyces cerivisiae was characterized for its site specific N-glycosylation through mass spectrometry. The N-glycopeptides were derived using non specific proteases and are analysed directly on liquid chromatography coupled to ion trap mass spectrometer in tandem mode. The evaluation of glycan fragment ions and the Y 1 ions (peptide+HexNAc) +n revealed the glycan sequence and the corresponding site of attachment. We observed the microheterogeneity in N-glycans such as Man 11-15 GlcNAc 2 at Asn 13 , Man 8-12 GlcNAc 2 at Asn 87 , Man 9-14 GlcNAc 2 at Asn 168 and phosphorylated Man 12-17 GlcNAc 2 as well as Man 11-16 GlcNAc 2 at Asn 368 . The presence of N-glycans with Man <18 GlcNAc 2 indicated that in vacuoles the steady release of mannose/phospho mannose residues from glycans occurs initially at Asn 13 or Asn 168 followed by at Asn 368 . However, glycans at Asn 87 which comprises Man 8-12 residues as reported earlier remain intact suggesting its inaccessibility for a similar processing. This in turn indicates the interaction of the glycan at Asn 87 with the polypeptide chain implicating it in the folding of the protein. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. How a regulator is preparing for reviewing a license application file: The case of ASN

    International Nuclear Information System (INIS)

    Tanguy, Loic

    2014-01-01

    The French Nuclear Safety Authority (ASN) is an independent administrative authority. It prepares regulation pertaining to the management of radioactive waste, monitors the control of safety of basic nuclear installations that produce or treat waste or are involved in their disposal and performs inspections of waste producers (EDF, AREVA, CEA, hospitals, research centres, etc.) and Andra, the French National Radioactive Waste Management Agency. It regulates the overall system set up by Andra for accepting waste from producers and assesses waste management policy and the practices of radioactive waste producers. It reviews license applications and authorises commissioning of nuclear installations. In order to review technical documents, ASN benefits from the expertise of technical support organisations. The French Institute for Radiation Protection and Nuclear Safety (IRSN) is the main such organisation. ASN has been making efforts to diversify its experts for several years. In preparing its decisions, ASN also calls on the opinions and recommendations of seven Advisory Committees of Experts (GPE), with expert knowledge in the areas of waste, nuclear pressure equipment, medical exposure, non-medical radiation protection, reactors, transport, and laboratories and nuclear plants. ASN consults the GPEs in preparing its main decisions. In particular, they review the preliminary, provisional and final safety analysis reports for each nuclear installation. They can also be consulted about changes in regulations or doctrine. (authors)

  3. Publication of the inspection follow-up letters of the ASN on its site web asn.gouv.fr; Publication des lettres de suite d'inspection de l'ASN sur son site web asn.gouv.fr

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    Since February 2002, the French authority of nuclear safety (ASN) publishes on its web site (http://www.asn.gouv.fr) the letters addressed to the operators of nuclear facilities at the end of its inspections. The ASN carries out about 700 inspections every year which concern the French nuclear facilities, the central services of nuclear operators or of their suppliers, and the transports of nuclear materials. Each inspection is followed by a follow-up letter which mentions all anomalies noticed during the inspection and eventually asks for some remedial actions or for some complements of information. This document brings together the letters published between february and May 2002 and concerning the on-site inspections of nuclear facilities (EdF nuclear power plants, CEA centers, Cogema facilities, other sites) and the off-site inspections (Andra, transports of nuclear materials). (J.S.)

  4. ASN report on the status of nuclear safety and radiation protection in France in 2015. Extracts

    International Nuclear Information System (INIS)

    2016-01-01

    After a recall of ASN missions, key figures and organisation, an overview of main events for 2015, and a presentation of the French law related to energy transition for a green growth, a first part of this huge document presents actions undertaken by the ASN in 2015 in different fields: ionizing radiations and risks for health and the environment, principles and actors of control of nuclear safety and radiation protection, regulation, control of nuclear activities and of exposures to ionizing radiations, emergency radiological and post-accidental situations, public information transparency and participation, international relationships, regional overview of nuclear safety and radiation protection. The second part addresses activities controlled by the ASN: medical uses of ionizing radiations, industrial, research and veterinary uses and source safety, transportation of radioactive materials, EDF nuclear power plants, installations related to nuclear fuel cycle, various industrial and research installations, safety of basic nuclear installation dismantling, radioactive wastes and polluted sites and soils

  5. Solid state chemistry of new polysulphides in A/Sn/S (A = Na, K, Rb ...

    Indian Academy of Sciences (India)

    Administrator

    Solid state chemistry of new polysulphides in A/Sn/S (A = Na, K,. Rb) systems. M SUSEELA DEVI and K VIDYASAGAR. Department of Chemistry, Indian Institute of Technology Madras,. Chennai 600 036, India. Ternary polychalcogenides containing chalocogen–chalcogen bonds are metastable compounds that have been ...

  6. New Promotion Patterns in Italian Universities: Less Seniority and More Productivity? Data from ASN

    Science.gov (United States)

    Marini, Giulio

    2017-01-01

    The new habilitation (ASN), established in Italy in 2010 and launched in 2012, was introduced to filter eligible candidates in the competition of associate and full professorships. Its purpose is to cut off poor candidates on the basis of individual scientific productivity before they might be hired in competitions where patronage may favor them.…

  7. Scanning Tunneling Microscopy study and unusual transport properties of the topological semimetal a-Sn

    Science.gov (United States)

    Ruan, Jiawei

    Weyl semimetals are new states of quantum matter with topological Weyl nodes near Fermi level in the bulk and Fermi arcs at the surface, which are paid a lot attention in recently years. Here#¬we report another topological semimetal a-Sn., which is double Weyl semimetal in the magnetic field and Dirac semimetal in an appropriate in-plane strain. By combing Landau level spectroscopy and quasiparticle interference, we obtain the linear dispersion near the Dirac point within strain while quadratic band dispersion near Γpoint without strain. We also observe the negative longitudinal magnetoresistance (LMR) in both two system, which is caused by chiral anomaly. However ,the LMR profiles of strained a-Sn have a little rise and then descend while the unstrained one drop directly, which is due to the different type of Weyl semimetal and further confirm our prediction.

  8. ASN report on the status of nuclear safety and radiation protection in France in 2011

    International Nuclear Information System (INIS)

    2012-01-01

    The first part of this huge report proposes a detailed overview of ASN activities in different fields: ionizing radiations related to nuclear activities and risks for health and the environment, principles and actors of nuclear safety control, of radiation protection and of protection of the environment, regulation, control of nuclear activities and exposures to ionizing radiations, emergency situations, public information and transparency, international relationships, regional overview of nuclear safety and radiation protection. The second part addresses the activities controlled by the ASN: medical use of ionizing radiations, non medical use of ionizing radiations, transport of radioactive materials, electronuclear power stations, facilities involved in the nuclear fuel cycle, research facilities and other nuclear installations, the safety of dismantling of nuclear base installations, radioactive wastes and polluted sites and soils

  9. ASN1-encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds.

    Science.gov (United States)

    Gaufichon, Laure; Marmagne, Anne; Belcram, Katia; Yoneyama, Tadakatsu; Sakakibara, Yukiko; Hase, Toshiharu; Grandjean, Olivier; Clément, Gilles; Citerne, Sylvie; Boutet-Mercey, Stéphanie; Masclaux-Daubresse, Céline; Chardon, Fabien; Soulay, Fabienne; Xu, Xiaole; Trassaert, Marion; Shakiebaei, Maryam; Najihi, Amina; Suzuki, Akira

    2017-08-01

    Despite a general view that asparagine synthetase generates asparagine as an amino acid for long-distance transport of nitrogen to sink organs, its role in nitrogen metabolic pathways in floral organs during seed nitrogen filling has remained undefined. We demonstrate that the onset of pollination in Arabidopsis induces selected genes for asparagine metabolism, namely ASN1 (At3g47340), GLN2 (At5g35630), GLU1 (At5g04140), AapAT2 (At5g19950), ASPGA1 (At5g08100) and ASPGB1 (At3g16150), particularly at the ovule stage (stage 0), accompanied by enhanced asparagine synthetase protein, asparagine and total amino acids. Immunolocalization confined asparagine synthetase to the vascular cells of the silique cell wall and septum, but also to the outer and inner seed integuments, demonstrating the post-phloem transport of asparagine in these cells to developing embryos. In the asn1 mutant, aberrant embryo cell divisions in upper suspensor cell layers from globular to heart stages assign a role for nitrogen in differentiating embryos within the ovary. Induction of asparagine metabolic genes by light/dark and nitrate supports fine shifts of nitrogen metabolic pathways. In transgenic Arabidopsis expressing promoter Ca MV 35S ::ASN1 fusion, marked metabolomics changes at stage 0, including a several-fold increase in free asparagine, are correlated to enhanced seed nitrogen. However, specific promoter Napin2S ::ASN1 expression during seed formation and a six-fold increase in asparagine toward the desiccation stage result in wild-type seed nitrogen, underlining that delayed accumulation of asparagine impairs the timing of its use by releasing amide and amino nitrogen. Transcript and metabolite profiles in floral organs match the carbon and nitrogen partitioning to generate energy via the tricarboxylic acid cycle, GABA shunt and phosphorylated serine synthetic pathway. © 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd.

  10. Management of long-lived radioactive waste: stakes and ASN actions

    International Nuclear Information System (INIS)

    Dandrieux, G.

    2011-01-01

    Due to the length of time it takes to decay, long-lived radioactive waste will remain a hazard on a timescale beyond the length of a human life, and even of several generations. In the case of this waste, long-term management solutions must be implemented to protect human health and the environment without requiring human intervention. In accordance with requirements under the Law, ANDRA (national agency for the management of radioactive waste) is carrying out research on disposal solutions in deep or subsurface geological formations. Nonetheless, until such repositories become available, ASN (authority for nuclear safety) has a duty to ensure safety at current and future interim storage facilities, as well as the safety of operations to retrieve and package this type of waste. To this end, ASN acts on several levels: it is involved in drawing up regulations and reference texts, at national and international level, it examines safety analysis reports related to radioactive waste management at basic nuclear installations, by means of inspections that it performs at every step in radioactive waste management. In its capacity as joint coordinator of the national plan for the management of nuclear waste and materials (PNGMDR) working group, ASN also plays a very active role in drawing up the provisions of the PNGMDR aimed at improving and optimising radioactive waste management. (author)

  11. The protein C omega-loop substitution Asn2Ile is associated with reduced protein C anticoagulant activity.

    LENUS (Irish Health Repository)

    Preston, Roger J S

    2012-02-01

    We report a kindred with heritable protein C (PC) deficiency in which two siblings with severe thrombosis showed a composite type I and IIb PC deficiency phenotype, identified using commercial PC assays (proband: PC antigen 42 u\\/dl, amidolytic activity 40 u\\/dl, anticoagulant activity 9 u\\/dl). The independent PROC nucleotide variations c.669C>A (predictive of Ser181Arg) and c.131C>T (predictive of Asn2Ile) segregated with the type I and type IIb PC deficiency phenotypes respectively, but co-segregated in the siblings with severe thrombosis. Soluble thrombomodulin (sTM)-mediated inhibition of plasma thrombin generation from an individual with PC-Asn2Ile was lower (endogenous thrombin potential (ETP) 56 +\\/- 1% that of ETP determined without sTM) than control plasma (ETP 15 +\\/- 2%) indicating reduced PC anticoagulant activity. Recombinant APC-Asn2Ile exhibited normal amidolytic activity but impaired anticoagulant activity. Protein S (PS)-dependent anticoagulant activity of recombinant APC-Asn2Ile and binding of recombinant APC-Asn2Ile to endothelial protein C receptor (EPCR) were reduced compared to recombinant wild-type APC. Asn2 lies within the omega-loop of the PC\\/APC Gla domain and this region is critical for calcium-induced folding and subsequent interactions with anionic phospholipids, EPCR and PS. The disruption of these interactions in this naturally-occurring PC variant highlights their collective importance in mediating APC anticoagulant activity in vivo.

  12. THE ANALYSIS TOWARDS STATE APPARATUS COMMISSION ROLE IN ASN MANAGEMENT SYSTEM IN INDONESIA

    Directory of Open Access Journals (Sweden)

    Ajib Rakhmawanto

    2015-05-01

    Full Text Available In administrating bureaurcy business process, professional state apparatus are needed. Law number 5 of 2014 about the state civil apparatus mentions management based on merit that supposed to be supervised by an independen institution: State Apparatuss Commision (KASN. This article aims to analyze the KASN, and identify its task, function, and responsibilities. The analysis showed that KASN was formed to create professional state apparatus and supervise the merit system based management. The function of KASN becomes ineffective considering there is NCSA, an establish body with a role to supervise the ASN management. KASN will cause an overlapping and conflict of interest between the two institutions.

  13. Assessment of patient radiation protection in external radiotherapy departments after inspections performed by the ASN 2008

    International Nuclear Information System (INIS)

    Franchi, Vincent; Marchal, Carole

    2009-10-01

    This report proposes an assessment of patient radiation protection in external radiotherapy. It is based on inter-regional syntheses of inspections performed by the ASN in external radiotherapy departments during 2008. It addresses 6 main themes related to patient radiation protection: human and material resources, organisation of medical physics, training in patient radiation protection, mastering of equipment (maintenance, internal quality controls of medical devices), safety and care quality management (formalization of the patient care process and definition of responsibilities, patient identity control, treatment preparation, and treatment execution), and risk management (a priori risk analysis, declaration, recording and internal processing of dysfunctions, improvements of care quality and safety management system)

  14. ASN.1 notation for exchange of data in computer-based railway control systems

    Directory of Open Access Journals (Sweden)

    Zbigniew ŁUKASIK

    2009-01-01

    Full Text Available Development of railway control systems aims at computerization. In most cases these systems are Distributed Real Time Systems. However, a huge problem in their putting into practice is the lack of interface standardization in the range of data structures and information exchange methods. It results in a variety of solutions, and thus in problems concerning cooperation of systems that come from different software vendors. Specification of protocols for data exchanging applications should therefore be created with the use of generally accepted standards. One of them is ASN.1 (Abstract Syntax Notation One language, which shall be presented in this article.

  15. Changes in FGFR2 amino-acid residue Asn549 lead to Crouzon and Pfeiffer syndrome with hydrocephalus

    Directory of Open Access Journals (Sweden)

    Caroline Apra

    2016-10-01

    Full Text Available Mutations in Fibroblast Growth Factor Receptor II (FGFR2 have been identified in patients with Crouzon and Pfeiffer syndrome, among which rare mutations of the intracellular tyrosine kinase domain. Correlating subtle phenotypes with each rare mutation is still in progress. In Necker-Enfants Malades Hospital, we identified three patients harboring three different pathogenic variants of the same amino acid residue Asn-549 located in this domain: in addition to a very typical crouzonoid appearance, they all developed clinically relevant hydrocephalus, which is an inconstant feature of Crouzon and Pfeiffer syndrome. Overall, FGFR2 tyrosine kinase domain mutations account for 5/67 (7.4% cases in our hospital. We describe a novel mutation, p.Asn549Ser, and new cases of p.Asn549His and p.Asn549Thr mutations, each reported once before. Our three cases of Asn-549 mutations, alongside with rare previously reported cases, show that these patients are at higher risk of hydrocephalus. Clinical and imaging follow-up, with possible early surgery, may help prevent secondary intellectual disability.

  16. Asparaginase treatment side-effects may be due to genes with homopolymeric Asn codons (Review-Hypothesis)

    Science.gov (United States)

    BANERJI, JULIAN

    2015-01-01

    The present treatment of childhood T-cell leukemias involves the systemic administration of prokary-otic L-asparaginase (ASNase), which depletes plasma Asparagine (Asn) and inhibits protein synthesis. The mechanism of therapeutic action of ASNase is poorly understood, as are the etiologies of the side-effects incurred by treatment. Protein expression from genes bearing Asn homopolymeric coding regions (N-hCR) may be particularly susceptible to Asn level fluctuation. In mammals, N-hCR are rare, short and conserved. In humans, misfunctions of genes encoding N-hCR are associated with a cluster of disorders that mimic ASNase therapy side-effects which include impaired glycemic control, dislipidemia, pancreatitis, compromised vascular integrity, and neurological dysfunction. This paper proposes that dysregulation of Asn homeostasis, potentially even by ASNase produced by the microbiome, may contribute to several clinically important syndromes by altering expression of N-hCR bearing genes. By altering amino acid abundance and modulating ribosome translocation rates at codon repeats, the microbiomic environment may contribute to genome decoding and to shaping the proteome. We suggest that impaired translation at poly Asn codons elevates diabetes risk and severity. PMID:26178806

  17. Asparaginase treatment side-effects may be due to genes with homopolymeric Asn codons (Review-Hypothesis).

    Science.gov (United States)

    Banerji, Julian

    2015-09-01

    The present treatment of childhood T-cell leukemias involves the systemic administration of prokaryotic L-asparaginase (ASNase), which depletes plasma Asparagine (Asn) and inhibits protein synthesis. The mechanism of therapeutic action of ASNase is poorly understood, as are the etiologies of the side-effects incurred by treatment. Protein expression from genes bearing Asn homopolymeric coding regions (N-hCR) may be particularly susceptible to Asn level fluctuation. In mammals, N-hCR are rare, short and conserved. In humans, misfunctions of genes encoding N-hCR are associated with a cluster of disorders that mimic ASNase therapy side-effects which include impaired glycemic control, dislipidemia, pancreatitis, compromised vascular integrity, and neurological dysfunction. This paper proposes that dysregulation of Asn homeostasis, potentially even by ASNase produced by the microbiome, may contribute to several clinically important syndromes by altering expression of N-hCR bearing genes. By altering amino acid abundance and modulating ribosome translocation rates at codon repeats, the microbiomic environment may contribute to genome decoding and to shaping the proteome. We suggest that impaired translation at poly Asn codons elevates diabetes risk and severity.

  18. Cyclization of the N-Terminal X-Asn-Gly Motif during Sample Preparation for Bottom-Up Proteomics

    DEFF Research Database (Denmark)

    Zhang, Xumin; Højrup, Peter

    2010-01-01

    We, herein, report a novel -17 Da peptide modification corresponding to an N-terminal cyclization of peptides possessing the N-terminal motif of X-Asn-Gly. The cyclization occurs spontaneously during sample preparation for bottom-up proteomics studies. Distinct from the two well-known N-terminal ......We, herein, report a novel -17 Da peptide modification corresponding to an N-terminal cyclization of peptides possessing the N-terminal motif of X-Asn-Gly. The cyclization occurs spontaneously during sample preparation for bottom-up proteomics studies. Distinct from the two well-known N......-terminal cyclizations, cyclization of N-terminal glutamine and S-carbamoylmethylcysteine, it is dependent on pH instead of [NH(4)(+)]. The data set from our recent study on large-scale N(α)-modified peptides revealed a sequence requirement for the cyclization event similar to the well-known deamidation of Asn to iso...

  19. Fessenheim 2: ASN's green light for continuing operation - Beginning of the works for unit 1

    International Nuclear Information System (INIS)

    Anon.

    2013-01-01

    Every 10 years a nuclear power plant operator has to make a re-assessment of the nuclear safety standard of his plant. This re-assessment is made of 2 parts: first the review of the safety conformity and secondly a thorough re-examination of the safety that takes into account today's safety standards and feedback experience from similar plants. This detailed assessment of the safety aims at checking that the consequences of the different aging phenomena are well mastered for the next 10 years at least. At the end of this re-assessment, the ASN (French Nuclear Safety Authorities) decide or not the continuation of plant activity or can prescribe safety improvements. In the case of the unit 2 of the Fessenheim plant that has just finished its third decennial safety re-assessment, the ASN has prescribed the same improvements as for the unit 1, that is to say the reinforcement of the resistance to corium of the foundation raft and an improvement on the emergency cooling system. The works on the unit 1 have begun despite contestation from anti-nuclear associations that question the cost of the safety upgrading (20 to 30 millions euros) while the unit is expected to be decommissioned by end 2016. (A.C.)

  20. Leukaemia risks and exposure to ionizing radiations. ASN seminar, Tuesday, June 9, 2015, report

    International Nuclear Information System (INIS)

    Niel, Jean-Christophe; Samain, Jean-Paul; Colonna, Marc; Maynadie, Marc; Richardson, David; Bey, Pierre; Leuraud, Klervi; Laurier, Dominique; Hemon, Denis; Spycher, Ben; Kosti, Ourania; Bouville, Andre; Grosche, Bernd; Ziegelberger, Gunde; Kesminiene, Ausrele; Clavel, Jacqueline; Smeesters, Patrick; Murith, Christophe

    2015-08-01

    This seminar aims at proposing a review of present knowledge on leukaemia risks for children and adults associated with ionizing radiations, and at sharing knowledge between experts. After an introduction which outlined the interest of the ASN in research issues, and the importance awarded by the ASN to the variety of points of view, a first session addressed leukaemia and exposures to ionizing radiations. The contributions addressed some general aspects (an overview of leukaemia in France, the different types of adult and child leukaemia), leukaemia and acute exposures to ionizing radiations (ionizing radiation and leukaemia among Japanese bomb survivors, risks of leukaemia after radiotherapy), leukaemia and chronic exposures to ionizing radiations (assessment of epidemiological studies for adult chronic exposures). The second session addressed childhood leukaemia and ionizing radiations. The contributions of this second session more particularly addressed the following topics: childhood leukaemia and natural radioactivity (French studies, synthesis of international studies and a new Swiss study), childhood leukaemia and proximity of nuclear base installations (assessment of national and international studies, analysis of cancer risks in populations near nuclear facilities in the US, calculation of dose at the medulla as example of dosimetry of ionizing radiations and leukaemia, conclusions of the 2012 MELODI workshop), childhood leukaemia and scanner (recent results and perspectives), childhood leukaemia and other risk factors (etiology of childhood leukaemia - presentation of French studies initiated by the INSERM, and presentation of studies initiated by BfS)

  1. Polymorphisms in XPD (Asp312Asn and Lys751Gln) genes, sunburn and arsenic-related skin lesions.

    Science.gov (United States)

    McCarty, Kathleen M; Smith, Thomas J; Zhou, Wei; Gonzalez, Ernesto; Quamruzzaman, Quazzi; Rahman, Mahmuder; Mahiuddin, Golam; Ryan, Louise; Su, Li; Christiani, David C

    2007-08-01

    Single-nucleotide polymorphisms in genes related to DNA repair capacity and ultraviolet exposure have not been well investigated in relation to skin lesions associated with arsenic exposure. This population based case-control study, of 600 cases and 600 controls, frequency matched on age and gender in Pabna, Bangladesh, in 2001-2002, investigated the association and potential effect modification between polymorphisms in Xeroderma Pigmentosum complementation group D (XPD) (Lys751Gln and Asp312Asn) genes, tendency to sunburn and arsenic-related skin lesions. Unconditional logistic regression was used to estimate odds ratios (ORs) and 95% confidence intervals (CIs). No significant association was observed between skin lesions and the XPD 312 Asp/Asn (adjusted OR = 0.87, 95% CI = 0.65-1.15) Asn/Asn (adjusted OR = 0.76, 95% CI = 0.50-1.15) (referent Asp/Asp); XPD 751 Lys/Gln (adjusted OR = 0.92, 95% CI = 0.69-1.23) Gln/Gln (adjusted OR = 0.98, 95% CI = 0.66-1.45) (referent Lys/Lys). While we did not observe any evidence of effect modification of these polymorphisms on the association between well arsenic concentration and skin lesions, we did observe effect modification between these polymorphisms and sunburn tendency and arsenic-related skin lesions. Individuals with the heterozygote or homozygote variant forms (Asp/Asn or Asn/Asn) had half the risk of skin lesions (OR = 0.45, 95% CI = 0.29-0.68) compared with those with the wild-type XPDAsp312Asn genotype (Asp/Asp) and individuals with heterozygote or homozygote variant forms (Lys/Gln or Gln/Gln) had half the risk of skin lesions (OR = 0.47, 95% CI = 0.31-0.72) compared with those with the wild-type XPDLys751Gln genotype (Lys/Lys), within the least sensitive strata of sunburn severity. We observed effect modification on the multiplicative scale for XPD 751 and XPD 312. XPD polymorphisms modified the relationship between tendency to sunburn and skin lesions in an arsenic exposed population. Further study is necessary

  2. Reports by the Parliamentary Office for scientific and technological assessment. Wednesday, March 30, 2011. 2010 activity report - Hearing of the Nuclear Safety Authority (ASN) college

    International Nuclear Information System (INIS)

    2011-03-01

    Members of the French Parliament and representatives of the French nuclear safety authority (ASN) discuss the content of the ASN 2010 activity report, the missions and responsibilities of the ASN in the different fields of exploitation of radioactive materials: nuclear energy and medical care. As far as nuclear energy is concerned, the representatives of the ASN evoke the decennial inspection of the French nuclear reactors, the control of the EPR construction, activities related to fuel cycle, the issue of waste management. As far as medical care is concerned, they address activities in the medical sector, the evolution of the number of events, and the commitment of the ASN in regulation elaboration. These issues are discussed with the members of Parliament

  3. Lack of association between urotensin-II (UTS2 gene polymorphisms (Thr21Met and Ser89Asn and migraine

    Directory of Open Access Journals (Sweden)

    Betül Ozan

    2017-08-01

    Full Text Available Migraine is a common neurovascular brain disorder with heterogeneous clinical presentation, including recurrent headache attacks. The pathophysiology of migraine is complex, and a number of genomic regions have been associated with the development of migraine. In this study, we analyzed the allele and genotype frequencies of the urotensin-II gene (UTS2 polymorphisms, Thr21Met and Ser89Asn, among Turkish patients with migraine. A total of 146 patients with migraine (14 with aura [MA group] and 132 without aura [MO group] were genotyped for Thr21Met and Ser89Asn polymorphisms and compared with 154 age- and sex-matched healthy controls. The UTS2 gene polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP. No significant differences were observed in allele and genotype frequencies for Thr21Met and Ser89Asn polymorphisms between the patients with migraine and control group. Similarly, we did not observe significant differences in allele and genotype frequencies between MA and MO and control group. Moreover, the haplotype analysis showed no association between UTS2 gene haplotypes (MN, MS, TN, and TS and migraine. In summary, Thr21Met and Ser89Asn polymorphisms of the UTS2 gene are not risk factors for migraine in our sample of Turkish migraine patients.

  4. A lipoprotein lipase mutation (Asn291Ser) is associated with reduced HDL cholesterol levels in premature atherosclerosis

    NARCIS (Netherlands)

    Reymer, P.W.A.; Gagné, S E; Groenemeyer, B E; Zhang, H; Forsyth, I; Jansen, H; Seidell, J C; Kromhout, D.; Lie, K E; Kastelein, J.J.

    1995-01-01

    A reduction of high density lipoprotein cholesterol (HDC) is recognized as an important risk factor for coronary artery disease (CAD). We now show in approximately 1 in 20 males with proven atherosclerosis that an Asn291Ser mutation in the human lipoprotein lipase (LPL) gene is associated with

  5. The ASN imposes on EDF complementary requirements for the setting of the hard-core unit

    International Nuclear Information System (INIS)

    Anon.

    2014-01-01

    In January 2014, the ASN (French Authority for Nuclear Safety) published a list of 19 decisions concerning the implementation of a 'post-Fukushima' hard-core unit in each EDF nuclear power plant. The purpose of the hard-core unit is to have structures and equipment able to resist very extreme events and able to assure the vital functions necessary for the reactor safety. The 19 decisions detail the rules for the design of the hard-core unit. For example the hard-core unit will have to resist earthquakes for which the probability of occurrence is about 1 every 20.000 years while the probability taking into account for the design of the reactor is only 1 every 1000 or 10.000 years

  6. Glycosylation at Asn91 of H1N1 haemagglutinin affects binding to glycan receptors.

    Science.gov (United States)

    Jayaraman, Akila; Koh, Xiaoying; Li, Jing; Raman, Rahul; Viswanathan, Karthik; Shriver, Zachary; Sasisekharan, Ram

    2012-06-15

    The glycoprotein HA (haemagglutinin) on the surface of influenza A virus plays a central role in recognition and binding to specific host cell-surface glycan receptors and in fusion of viral membrane to the host nuclear membrane during viral replication. Given the abundance of HA on the viral surface, this protein is also the primary target for host innate and adaptive immune responses. Although addition of glycosylation sites on HA are a part of viral evolution to evade the host immune responses, there are specific glycosylation sites that are conserved during most of the evolution of the virus. In the present study, it was demonstrated that one such conserved glycosylation site at Asn(91) in H1N1 HA critically governs the glycan receptor-binding specificity and hence would potentially impinge on the host adaptation of the virus.

  7. ASN guide project. Safety policy and management in INBs (base nuclear installations)

    International Nuclear Information System (INIS)

    2010-01-01

    This guide presents the recommendations of the French Nuclear Safety Authority (ASN) in the field of safety policy and management (PMS) for base nuclear installations (INBs). It gives an overview and comments of some prescriptions of the so-called INB order and PMS decision. These regulatory texts define a framework for provisions any INB operator must implement to establish his safety policy, to define and implement a system which allows the safety to be maintained, the improvement of his INB safety to be permanently looked for. The following issues are addressed: operator's safety policy, identification of elements important for safety, of activities pertaining to safety, and of associated requirements, safety management organization and system, management of activities pertaining to safety, documentation and archiving

  8. Mutation of Asn28 Disrupts the Dimerization and Enzymatic Activity of SARS 3CL

    Energy Technology Data Exchange (ETDEWEB)

    Barrila, J.; Gabelli, S; Bacha, U; Amzel, M; Freire, E

    2010-01-01

    Coronaviruses are responsible for a significant proportion of annual respiratory and enteric infections in humans and other mammals. The most prominent of these viruses is the severe acute respiratory syndrome coronavirus (SARS-CoV) which causes acute respiratory and gastrointestinal infection in humans. The coronavirus main protease, 3CL{sup pro}, is a key target for broad-spectrum antiviral development because of its critical role in viral maturation and high degree of structural conservation among coronaviruses. Dimerization is an indispensable requirement for the function of SARS 3CL{sup pro} and is regulated through mechanisms involving both direct and long-range interactions in the enzyme. While many of the binding interactions at the dimerization interface have been extensively studied, those that are important for long-range control are not well-understood. Characterization of these dimerization mechanisms is important for the structure-based design of new treatments targeting coronavirus-based infections. Here we report that Asn28, a residue 11 {angstrom} from the closest residue in the opposing monomer, is essential for the enzymatic activity and dimerization of SARS 3CLpro. Mutation of this residue to alanine almost completely inactivates the enzyme and results in a 19.2-fold decrease in the dimerization K{sub d}. The crystallographic structure of the N28A mutant determined at 2.35 {angstrom} resolution reveals the critical role of Asn28 in maintaining the structural integrity of the active site and in orienting key residues involved in binding at the dimer interface and substrate catalysis. These findings provide deeper insight into complex mechanisms regulating the activity and dimerization of SARS 3CL{sup pro}.

  9. HOST GALAXY PROPERTIES OF THE SUBLUMINOUS GRB 120422A/SN 2012bz

    Energy Technology Data Exchange (ETDEWEB)

    Levesque, Emily M. [CASA, Department of Astrophysical and Planetary Sciences, University of Colorado 389-UCB, Boulder, CO 80309 (United States); Chornock, Ryan; Soderberg, Alicia M.; Berger, Edo; Lunnan, Ragnhild, E-mail: Emily.Levesque@colorado.edu [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States)

    2012-10-20

    GRB 120422A is a nearby (z = 0.283) long-duration gamma-ray burst (LGRB) detected by Swift with E {sub {gamma},iso} {approx} 4.5 Multiplication-Sign 10{sup 49} erg. It is also associated with the spectroscopically confirmed broad-lined Type Ic SN 2012bz. These properties establish GRB 120422A/SN 2012bz as the sixth and newest member of the class of subluminous GRBs supernovae (SNe). Observations also show that GRB 120422A/SN 2012bz occurred at an unusually large offset ({approx}8 kpc) from the host galaxy nucleus, setting it apart from other nearby LGRBs and leading to speculation that the host environment may have undergone prior interaction activity. Here, we present spectroscopic observations using the 6.5 m Magellan telescope at Las Campanas. We extract spectra at three specific locations within the GRB/SN host galaxy, including the host nucleus, the explosion site, and the 'bridge' of diffuse emission connecting these two regions. We measure a metallicity of log(O/H) + 12 = 8.3 {+-} 0.1 and a star formation rate (SFR) per unit area of 0.08 M {sub Sun} yr{sup -1} kpc{sup -2} at the host nucleus. At the GRB/SN explosion site we measure a comparable metallicity of log(O/H) + 12 = 8.2 {+-} 0.1 but find a much lower SFR per unit area of 0.01 M {sub Sun} yr{sup -1} kpc{sup -2}. We also compare the host galaxy of this event to the hosts of other LGRBs, including samples of subluminous LGRBs and cosmological LGRBs, and find no systematic metallicity difference between the environments of these different subtypes.

  10. Transposon mutagenesis of probiotic Lactobacillus casei identifies asnH, an asparagine synthetase gene involved in its immune-activating capacity.

    Science.gov (United States)

    Ito, Masahiro; Kim, Yun-Gi; Tsuji, Hirokazu; Takahashi, Takuya; Kiwaki, Mayumi; Nomoto, Koji; Danbara, Hirofumi; Okada, Nobuhiko

    2014-01-01

    Lactobacillus casei ATCC 27139 enhances host innate immunity, and the J1 phage-resistant mutants of this strain lose the activity. A transposon insertion mutant library of L. casei ATCC 27139 was constructed, and nine J1 phage-resistant mutants out of them were obtained. Cloning and sequencing analyses identified three independent genes that were disrupted by insertion of the transposon element: asnH, encoding asparagine synthetase, and dnaJ and dnaK, encoding the molecular chaperones DnaJ and DnaK, respectively. Using an in vivo mouse model of Listeria infection, only asnH mutant showed deficiency in their ability to enhance host innate immunity, and complementation of the mutation by introduction of the wild-type asnH in the mutant strain recovered the immuno-augmenting activity. AsnH protein exhibited asparagine synthetase activity when the lysozyme-treated cell wall extracts of L. casei ATCC 27139 was added as substrate. The asnH mutants lost the thick and rigid peptidoglycan features that are characteristic to the wild-type cells, indicating that AsnH of L. casei is involved in peptidoglycan biosynthesis. These results indicate that asnH is required for the construction of the peptidoglycan composition involved in the immune-activating capacity of L. casei ATCC 27139.

  11. Transposon mutagenesis of probiotic Lactobacillus casei identifies asnH, an asparagine synthetase gene involved in its immune-activating capacity.

    Directory of Open Access Journals (Sweden)

    Masahiro Ito

    Full Text Available Lactobacillus casei ATCC 27139 enhances host innate immunity, and the J1 phage-resistant mutants of this strain lose the activity. A transposon insertion mutant library of L. casei ATCC 27139 was constructed, and nine J1 phage-resistant mutants out of them were obtained. Cloning and sequencing analyses identified three independent genes that were disrupted by insertion of the transposon element: asnH, encoding asparagine synthetase, and dnaJ and dnaK, encoding the molecular chaperones DnaJ and DnaK, respectively. Using an in vivo mouse model of Listeria infection, only asnH mutant showed deficiency in their ability to enhance host innate immunity, and complementation of the mutation by introduction of the wild-type asnH in the mutant strain recovered the immuno-augmenting activity. AsnH protein exhibited asparagine synthetase activity when the lysozyme-treated cell wall extracts of L. casei ATCC 27139 was added as substrate. The asnH mutants lost the thick and rigid peptidoglycan features that are characteristic to the wild-type cells, indicating that AsnH of L. casei is involved in peptidoglycan biosynthesis. These results indicate that asnH is required for the construction of the peptidoglycan composition involved in the immune-activating capacity of L. casei ATCC 27139.

  12. Significant event of patient radiation protection in radiotherapy (criterion 2.1): declaration and rating on the ASN-SFRO scale - ASN guide nr 16 - Release of the 01/01/2010

    International Nuclear Information System (INIS)

    2010-01-01

    This document brings together all the tools for the management of a significant event in radiation protection concerning a patient in radiotherapy. It contains the declaration form, a model for the reporting of significant events, and the ASN-SFRO scale which allows the event to be rated. After a presentation of the regulatory context and references, of the scope of application, this guide addresses the declaration of a significant event: general principles, definition of the 2.1 criterion, examples, delays and modalities of declaration, addressees of a declaration. It addresses the reporting of a significant event: general principles, sending delays and modalities. It addresses the rating on the ASN-SFRO scale: general principles, scale, and rating process. The last part deals with public information

  13. Significant event of patient radiation protection in radiotherapy (criterion 2.1): declaration and rating on the ASN-SFRO scale - ASN guide no. 16 - Release of 17/07/2015

    International Nuclear Information System (INIS)

    2015-01-01

    This document is an update of the previous version from October 2010. It brings together all the tools for the management of a significant event in radiation protection concerning a patient in radiotherapy. It contains the declaration form, a model for the reporting of significant events, and the ASN-SFRO scale which allows the event to be rated. After a presentation of the regulatory context and references, of the scope of application, this guide addresses the declaration of a significant event: general principles, definition of the 2.1 criterion, examples, delays and modalities of declaration, addressees of a declaration. It addresses the reporting of a significant event: general principles, sending delays and modalities. It addresses the rating on the ASN-SFRO scale: general principles, scale, and rating process. The last part deals with public information

  14. Reports by the Parliamentary Office for scientific and technological assessment. Wednesday, March 30, 2011. 2010 activity report - Hearing of the Nuclear Safety Authority (ASN) college; Comptes rendus de l' Office Parlementaire d'Evaluation des Choix Scientifiques et Technologiques. Mercredi 30 mars 2011. Rapport d'activite 2010 - Audition du college de l'Autorite de surete nucleaire (ASN)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2011-03-15

    Members of the French Parliament and representatives of the French nuclear safety authority (ASN) discuss the content of the ASN 2010 activity report, the missions and responsibilities of the ASN in the different fields of exploitation of radioactive materials: nuclear energy and medical care. As far as nuclear energy is concerned, the representatives of the ASN evoke the decennial inspection of the French nuclear reactors, the control of the EPR construction, activities related to fuel cycle, the issue of waste management. As far as medical care is concerned, they address activities in the medical sector, the evolution of the number of events, and the commitment of the ASN in regulation elaboration. These issues are discussed with the members of Parliament

  15. Asn792 participates in the hydrogen bond network around the K+-binding pocket of gastric H,K-ATPase.

    NARCIS (Netherlands)

    Swarts, H.G.P.; Koenderink, J.B.; Willems, P.H.G.M.; Krieger, E.; Pont, J.J.H.H.M. de

    2005-01-01

    Asn792 present in M5 of gastric H,K-ATPase is highly conserved within the P-type ATPase family. A direct role in K+ binding was postulated for Na,K-ATPase but was not found in a recent model for gastric H,K-ATPase (Koenderink, J. B., Swarts, H. G. P., Willems, P. H. G. M., Krieger, E., and De Pont,

  16. The ASN and nuclear facilities: towards a strengthening of safety margins

    International Nuclear Information System (INIS)

    Anon.

    2012-01-01

    The new measures taken by the French Nuclear Safety Authority (ASN) as a consequence of the complementary safety assessments performed recently on nuclear facilities are reviewed in this article. The main measures are the following. Concerning EDF: -) the setting of a hard core of measures in order to assure a few safety-vital functions in any case, for instance one of this measures is to bunker some of the emergency diesel sets; -) the setting of a rapid intervention force able to provide any damaged nuclear plant with extra means in cooling and power in a very short time; -) a better training of the staff in case of severe accident. Concerning AREVA: the setting of robust means to assure the water supply of the fuel pool at La Hague plant and the setting of efficient means to mitigate the consequences of a leak of ClF 3 , HF, UF 6 in the premises of Eurodif, Socatri, TU5, GB 2 and Comurhex. Concerning CEA: -) the removal of fissile materials from the Masurca facility, -) the setting up of improved means concerning flood and sodium fires at the Phenix reactor, -) the setting up of improved means concerning the loss of coolant at the Osiris reactor, -) the setting up of improved means in case of flood, earthquake and loss of coolant at the Jules Horowitz reactor. (A.C.)

  17. ASN: regional day of information and exchange on transport of radioactive materials - 4 February 2010

    International Nuclear Information System (INIS)

    2010-01-01

    This document gathers Power Point presentations made during a meeting on the transport of radioactive materials. The contributions addressed the following topics: results of a survey based on questionnaires sent to actors of this sector, regulatory framework and radioprotection plan, case of parcels not submitted to the authority concerned, declaration of transport interesting or significant events, ASN inspections, the transport of radioactive products by the IBt Bebig company in France, the activities of the Institut de Soudure Industrie (industry welding institute), the activities of the ISO Life company specialized in health product transportation, the activities of the Securidis company (a consulting company for activities related to hazardous materials transportation, and notably radioactive materials), the activities a the ACE Environnement company (specialized in building diagnosis), the point of view of an academic research unit on radioactive material transportation, the experience of the hospital sector in radioactive source transportation, the experience of the Advanced Accelerator Applications company in radioactive material transportation, and a discussion on regulation application

  18. From crisis exercises to the management of emergency situations...The experience of a local branch of ASN

    International Nuclear Information System (INIS)

    Deproit, L.; Vieuble, J.

    2016-01-01

    The local branch of the ASN (Nuclear Safety Authority) in the Marseille region controls the nuclear activities in 13 departments and Corsica. The preparation of the crisis management relies on the involvement of various actors: representatives of public bodies, nuclear plant operators, the ASN and its technical assistance (IRSN - Institute for Radiation-protection and Nuclear Safety). Crisis exercises are necessary to check the right coordination of all the actors. The feedback of real situations involving incidents are also important because it allows all the actors to better know each other, the case of the accident of the Centraco facility is detailed as well as the robbery of a truck carrying a radioactive source or the discovery of a collection of radioactive sources in a private home. In each case the lesson learned by the local branch of ASN is to communicate quickly with local authorities in order to get the best technical response to the situation and to give authorities all the information necessary for an efficient information of the public. (A.C.)

  19. N-linked glycosylation at Asn152 on CD147 affects protein folding and stability: promoting tumour metastasis in hepatocellular carcinoma.

    Science.gov (United States)

    Li, Jiang-Hua; Huang, Wan; Lin, Peng; Wu, Bo; Fu, Zhi-Guang; Shen, Hao-Miao; Jing, Lin; Liu, Zhen-Yu; Zhou, Yang; Meng, Yao; Xu, Bao-Qing; Chen, Zhi-Nan; Jiang, Jian-Li

    2016-11-21

    Cluster of differentiation 147 (CD147), also known as extracellular matrix metalloproteinase inducer, is a transmembrane glycoprotein that mediates oncogenic processes partly through N-glycosylation modifications. N-glycosylation has been demonstrated to be instrumental for the regulation of CD147 function during malignant transformation. However, the role that site-specific glycosylation of CD147 plays in its defective function in hepatocellular carcinomacells needs to be determined. Here, we demonstrate that the modification of N-glycosylation at Asn152 on CD147 strongly promotes hepatocellular carcinoma (HCC) invasion and migration. After the removal of N-glycans at Asn152, CD147 was more susceptible to degradation by ER-localized ubiquitin ligase-mediated endoplasmic reticulum-associated degradation (ERAD). Furthermore, N-linked glycans at Asn152 were required for CD147 to acquire and maintain proper folding in the ER. Moreover, N-linked glycans at Asn152 functioned as a recognition motif that was directly mediated by the CNX quality control system. Two phases in the retention-based ER chaperones system drove ER-localized CD147 trafficking to degradation. Deletion of N-linked glycosylation at Asn152 on CD147 significantly suppressed in situ tumour metastasis. These data could potentially shed light on the molecular regulation of CD147 through glycosylation and provide a valuable means of developing drugs that target N-glycans at Asn152 on CD147.

  20. Genetic polymorphisms of xeroderma pigmentosum group D gene Asp312Asn and Lys751Gln and susceptibility to prostate cancer: a systematic review and meta-analysis.

    Science.gov (United States)

    Ma, Qingtong; Qi, Can; Tie, Chong; Guo, Zhanjun

    2013-11-10

    Many studies have reported the role of xeroderma pigmentosum group D (XPD) with prostate cancer risk, but the results remained controversial. To derive a more precise estimation of the relationship, a meta-analysis was performed. Odds ratios (ORs) with 95% confidence intervals (CIs) were estimated to assess the association between XPD Asp312Asn and Lys751Gln polymorphisms and prostate cancer risk. A total of 8 studies including 2620 cases and 3225 controls described Asp312Asn genotypes, among which 10 articles involving 3230 cases and 3582 controls described Lys751Gln genotypes and were also involved in this meta-analysis. When all the eligible studies were pooled into this meta-analysis, a significant association between prostate cancer risk and XPD Asp312Asn polymorphism was found. For Asp312Asn polymorphism, in the stratified analysis by ethnicity and source of controls, prostate cancer risk was observed in co-dominant, dominant and recessive models, while no evidence of any associations of XPD Lys751Gln polymorphism with prostate cancer was found in the overall or subgroup analyses. Our meta-analysis supports that the XPD Asp312Asn polymorphism contributed to the risk of prostate cancer from currently available evidence. However, a study with a larger sample size is needed to further evaluate gene-environment interaction on XPD Asp312Asn and Lys751Gln polymorphisms and prostate cancer risk. © 2013.

  1. 2675-IJBCS-Article-Fongang Hensel Guillaume

    African Journals Online (AJOL)

    hp

    30 juil. 2013 ... Transformation et commercialisation des chips de banane plantain au ... plantain reste encore artisanale et familiale avec un prix moyen de vente de 100 FCFA le sachet. ..... que le mode de transfert de la connaissance.

  2. Effects of mutation of Asn694 in Aspergillus niger α-glucosidase on hydrolysis and transglucosylation.

    Science.gov (United States)

    Ma, Min; Okuyama, Masayuki; Sato, Megumi; Tagami, Takayoshi; Klahan, Patcharapa; Kumagai, Yuya; Mori, Haruhide; Kimura, Atsuo

    2017-08-01

    Aspergillus niger α-glucosidase (ANG), a member of glycoside hydrolase family 31, catalyzes hydrolysis of α-glucosidic linkages at the non-reducing end. In the presence of high concentrations of maltose, the enzyme also catalyzes the formation of α-(1→6)-glucosyl products by transglucosylation and it is used for production of the industrially useful panose and isomaltooligosaccharides. The initial transglucosylation by wild-type ANG in the presence of 100 mM maltose [Glc(α1-4)Glc] yields both α-(1→6)- and α-(1→4)-glucosidic linkages, the latter constituting ~25% of the total transfer reaction product. The maltotriose [Glc(α1-4)Glc(α1-4)Glc], α-(1→4)-glucosyl product disappears quickly, whereas the α-(1→6)-glucosyl products panose [Glc(α1-6)Glc(α1-4)Glc], isomaltose [Glc(α1-6)Glc], and isomaltotriose [Glc(α1-6)Glc(α1-6)Glc] accumulate. To modify the transglucosylation properties of ANG, residue Asn694, which was predicted to be involved in formation of the plus subsites of ANG, was replaced with Ala, Leu, Phe, and Trp. Except for N694A, the mutations enhanced the initial velocity of the α-(1→4)-transfer reaction to produce maltotriose, which was then degraded at a rate similar to that by wild-type ANG. With increasing reaction time, N694F and N694W mutations led to the accumulation of larger amounts of isomaltose and isomaltotriose than achieved with the wild-type enzyme. In the final stage of the reaction, the major product was panose (N694A and N694L) or isomaltose (N694F and N694W).

  3. Synthesis of the report from the IRRS evaluation mission performed at the ASN in April 2009 by 12 international experts under the IAEA coordination

    International Nuclear Information System (INIS)

    2009-01-01

    At the request of the Government authorities of France, an international team of 24 experts visited the Autorite de Surete Nucleaire (ASN), the French regulatory authority for nuclear and radiation safety, in November 2006 to conduct the first full scope Integrated Regulatory Review Service (IRRS) mission. The purpose of the mission was to undertake a peer review of the regulatory body of France against the IAEA Safety Standards and to exchange information and experience on safety regulation. In March 2008 the Government authorities of France requested a follow-up mission to review the measures undertaken following the recommendations and suggestions presented in the report of the November 2006 IRRS mission. The scope of the IRRS follow-up mission covered the regulatory aspects of the facilities and practices regulated by ASN, nuclear power plants, research reactors, fuel cycle facilities, medical practices with further review of radiotherapy, industrial and research activities, waste facilities, decommissioning, remediation, public information and communication and, in addition, it was also extended to cover the application of the Code of Conduct of Safety and Security of Radioactive Sources. The review was conducted from March 29 to April 3 2009 by an IRRS team consisting of 12 senior regulatory experts from 11 Member States, two staff members from the IAEA, one IAEA observer and an IAEA administrative assistant. During the review the team recognized that ASN has taken a number of initiatives to improve its effectiveness and efficiency and that ASN faces new challenges. ASN supplied a package of documentation and a well prepared self-assessment, in advance of the mission, including a status report and an action plan to improve its regulatory effectiveness. Both regulatory technical and policy issues were addressed. The policy issues discussed were: regulatory independence, the relationship between ASN and IRSN and medical issues. The IRRS follow-up mission

  4. Ser95, Asn97, and Thr78 are important for the catalytic function of porcine NADP-dependent isocitrate dehydrogenase

    OpenAIRE

    Kim, Tae-Kang; Colman, Roberta F.

    2005-01-01

    The mammalian mitochondrial NADP-dependent isocitrate dehydrogenase is a citric acid cycle enzyme and an important contributor to cellular defense against oxidative stress. The Mn2+-isocitrate complex of the porcine enzyme was recently crystallized; its structure indicates that Ser95, Asn97, and Thr78 are within hydrogen-bonding distance of the γ-carboxylate of enzyme-bound isocitrate. We used site-directed mutagenesis to replace each of these residues by Ala and Asp. The wild-type and mutant...

  5. Cell Cycle-Dependent Recruitment of Polycomb Proteins to the ASNS Promoter Counteracts C/ebp-Mediated Transcriptional Activation in Bombyx mori

    Science.gov (United States)

    Li, Zhiqing; Cheng, Daojun; Mon, Hiroaki; Zhu, Li; Xu, Jian; Tatsuke, Tsuneyuki; Lee, Jae Man; Xia, Qingyou; Kusakabe, Takahiro

    2013-01-01

    Epigenetic modifiers and transcription factors contribute to developmentally programmed gene expression. Here, we establish a functional link between epigenetic regulation by Polycomb group (PcG) proteins and transcriptional regulation by C/ebp that orchestrates the correct expression of Bombyx mori asparagine synthetase (BmASNS), a gene involved in the biosynthesis of asparagine. We show that the cis-regulatory elements of YY1-binding motifs and the CpG island present on the BmASNS promoter are required for the recruitment of PcG proteins and the subsequent deposition of the epigenetic repression mark H3K27me3. RNAi-mediated knockdown of PcG genes leads to derepression of the BmASNS gene via the recruitment of activators, including BmC/ebp, to the promoter. Intriguingly, we find that PcG proteins and BmC/ebp can dynamically modulate the transcriptional output of the BmASNS target in a cell cycle-dependent manner. It will be essential to suppress BmASNS expression by PcG proteins at the G2/M phase of the cell cycle in the presence of BmC/ebp activator. Thus, our results provide a novel insight into the molecular mechanism underlying the recruitment and regulation of the PcG system at a discrete gene locus in Bombyx mori. PMID:23382816

  6. The nuclear safety authority (ASN) presents its report on the status of nuclear safety and radiation protection in France in 2010

    International Nuclear Information System (INIS)

    2011-01-01

    After a presentation of the French nuclear safety authority (ASN) and of some events which occurred in 2010, this report present the actions performed by the ASN in different fields: nuclear activities (ionizing radiations and risks for health and for the environment), principles and actors of control of nuclear safety, radiation protection and environment protection, regulation, control of nuclear activities and of exposures to ionizing radiations, emergency situations, public information and transparency, international relationship. It proposes a regional overview of nuclear safety and radiation protection in France. It addresses the activities controlled by the ASN: medical and non medical usages of ionizing radiations, transportation of radioactive materials, electronuclear power stations, installations involved in the nuclear fuel cycle, research nuclear installations and other nuclear installations, safety in basic nuclear installation dismantling, radioactive wastes and polluted sites

  7. Characterization of deamidation at Asn138 in L-chain of recombinant humanized Fab expressed from Pichia pastoris.

    Science.gov (United States)

    Ohkuri, Takatoshi; Murase, Eri; Sun, Shu-Lan; Sugitani, Jun; Ueda, Tadashi

    2013-10-01

    A method was previously established for evaluating Asn deamidation by matrix-assisted laser desorption/ionization time of flight-mass spectrometry using endoproteinase Asp-N. In this study, we demonstrated that this method could be applied to the identification of the deamidation site of the humanized fragment antigen-binding (Fab). First, a system for expressing humanized Fab from methylotrophic yeast Pichia pastoris was constructed, resulting in the preparation of ∼30 mg of the purified humanized Fab from 1 l culture. Analysis of the L-chain derived from recombinant humanized Fab that was heated at pH 7 and 100°C for 1 h showed the deamidation at Asn138 in the constant region. Then, we prepared L-N138D Fab and L-N138A Fab and examined their properties. The circular dichroism (CD) spectrum of the L-N138D Fab was partially different from that of the wild-type Fab. The measurement of the thermostability showed that L-N138D caused a significant decrease in the thermostability of Fab. On the other hand, the CD spectrum and thermostability of L-N138A Fab showed the same behaviour as the wild-type Fab. Thus, it was suggested that the introduction of a negative charge at position 138 in the L-chain by the deamidation significantly affected the stability of humanized Fab.

  8. Preventing E-cadherin aberrant N-glycosylation at Asn-554 improves its critical function in gastric cancer

    Science.gov (United States)

    Carvalho, S; Catarino, TA; Dias, AM; Kato, M; Almeida, A; Hessling, B; Figueiredo, J; Gärtner, F; Sanches, JM; Ruppert, T; Miyoshi, E; Pierce, M; Carneiro, F; Kolarich, D; Seruca, R; Yamaguchi, Y; Taniguchi, N; Reis, CA; Pinho, SS

    2016-01-01

    E-cadherin is a central molecule in the process of gastric carcinogenesis and its posttranslational modifications by N-glycosylation have been described to induce a deleterious effect on cell adhesion associated with tumor cell invasion. However, the role that site-specific glycosylation of E-cadherin has in its defective function in gastric cancer cells needs to be determined. Using transgenic mice models and human clinical samples, we demonstrated that N-acetylglucosaminyltransferase V (GnT-V)-mediated glycosylation causes an abnormal pattern of E-cadherin expression in the gastric mucosa. In vitro models further indicated that, among the four potential N-glycosylation sites of E-cadherin, Asn-554 is the key site that is selectively modified with β1,6 GlcNAc-branched N-glycans catalyzed by GnT-V. This aberrant glycan modification on this specific asparagine site of E-cadherin was demonstrated to affect its critical functions in gastric cancer cells by affecting E-cadherin cellular localization, cis-dimer formation, molecular assembly and stability of the adherens junctions and cell–cell aggregation, which was further observed in human gastric carcinomas. Interestingly, manipulating this site-specific glycosylation, by preventing Asn-554 from receiving the deleterious branched structures, either by a mutation or by silencing GnT-V, resulted in a protective effect on E-cadherin, precluding its functional dysregulation and contributing to tumor suppression. PMID:26189796

  9. Characterization of an Lrp/AsnC family regulator SCO3361, controlling actinorhodin production and morphological development in Streptomyces coelicolor.

    Science.gov (United States)

    Liu, Jing; Li, Jie; Dong, Hong; Chen, Yunfu; Wang, Yansheng; Wu, Hang; Li, Changrun; Weaver, David T; Zhang, Lixin; Zhang, Buchang

    2017-07-01

    Lrp/AsnC family regulators have been found in many bacteria as crucial regulators controlling diverse cellular processes. By genomic alignment, we found that SCO3361, an Lrp/AsnC family protein from Streptomyces coelicolor, shared the highest similarity to the SACE_Lrp from Saccharopolyspora erythraea. Deletion of SCO3361 led to dramatic reduction in actinorhodin (Act) production and delay in aerial mycelium formation and sporulation on solid media. Dissection of the mechanism underlying the function of SCO3361 in Act production revealed that it altered the transcription of the cluster-situated regulator gene actII-ORF4 by directly binding to its promoter. SCO3361 was an auto-regulator and simultaneously activated the transcription of its adjacent divergently transcribed gene SCO3362. SCO3361 affected aerial hyphae formation and sporulation of S. coelicolor by activating the expression of amfC, whiB, and ssgB. Phenylalanine and cysteine were identified as the effector molecules of SCO3361, with phenylalanine reducing the binding affinity, whereas cysteine increasing it. Moreover, interactional regulation between SCO3361 and SACE_Lrp was discovered for binding to each other's target gene promoter in this work. Our findings indicate that SCO3361 functions as a pleiotropic regulator controlling secondary metabolism and morphological development in S. coelicolor.

  10. Report by the ASN on the situation of nuclear safety and radiation protection in France in 2013

    International Nuclear Information System (INIS)

    Chevet, Pierre-Franck; Delmestre, Alain; Bardet, Marie-Christine; Covard, Fabienne; Javay, Olivier

    2014-01-01

    In its first chapters, this huge report presents the various actions undertaken by the ASN. It proposes a detailed overview of the issue of ionizing radiations and risks for health and for the environment in the field of nuclear activities, presents and comments the principles and actors of the control of nuclear safety and of radiation protection, presents the different elements of the legal framework, describes and comments the control of nuclear activities and of exposures to ionizing radiations. The next chapters address the actions controlled by the ASN: radiological and post-accidental radiological situations, present measures related to public information and transparency, overview of international relationships, regional overview of nuclear safety and radiation protection, medical use of ionizing radiations, industrial, research and veterinary uses and the issue of source safety, the issue of radioactive material transportation. Issues of nuclear safety and radiation protection are then discussed for electronuclear plants, for installations dedicated to the nuclear fuel cycle, for various research and industrial nuclear installations, for the dismantling of base nuclear installations, and for radioactive wastes and polluted sites and soils

  11. Bioremediation of acidic oily sludge-contaminated soil by the novel yeast strain Candida digboiensis TERI ASN6.

    Science.gov (United States)

    Sood, Nitu; Patle, Sonali; Lal, Banwari

    2010-03-01

    Primitive wax refining techniques had resulted in almost 50,000 tonnes of acidic oily sludge (pH 1-3) being accumulated inside the Digboi refinery premises in Assam state, northeast India. A novel yeast species Candida digboiensis TERI ASN6 was obtained that could degrade the acidic petroleum hydrocarbons at pH 3 under laboratory conditions. The aim of this study was to evaluate the degradation potential of this strain under laboratory and field conditions. The ability of TERI ASN6 to degrade the hydrocarbons found in the acidic oily sludge was established by gravimetry and gas chromatography-mass spectroscopy. Following this, a feasibility study was done, on site, to study various treatments for the remediation of the acidic sludge. Among the treatments, the application of C. digboiensis TERI ASN6 with nutrients showed the highest degradation of the acidic oily sludge. This treatment was then selected for the full-scale bioremediation study conducted on site, inside the refinery premises. The novel yeast strain TERI ASN6 could degrade 40 mg of eicosane in 50 ml of minimal salts medium in 10 days and 72% of heneicosane in 192 h at pH 3. The degradation of alkanes yielded monocarboxylic acid intermediates while the polycyclic aromatic hydrocarbon pyrene found in the acidic oily sludge yielded the oxygenated intermediate pyrenol. In the feasibility study, the application of TERI ASN6 with nutrients showed a reduction of solvent extractable total petroleum hydrocarbon (TPH) from 160 to 28.81 g kg(-1) soil as compared to a TPH reduction from 183.85 to 151.10 g kg(-1) soil in the untreated control in 135 days. The full-scale bioremediation study in a 3,280-m(2) area in the refinery showed a reduction of TPH from 184.06 to 7.96 g kg(-1) soil in 175 days. Degradation of petroleum hydrocarbons by microbes is a well-known phenomenon, but most microbes are unable to withstand the low pH conditions found in Digboi refinery. The strain C. digboiensis could efficiently degrade

  12. The role of a second-shell residue in modifying substrate and inhibitor interactions in the SHV beta-lactamase: a study of ambler position Asn276.

    Science.gov (United States)

    Drawz, Sarah M; Bethel, Christopher R; Hujer, Kristine M; Hurless, Kelly N; Distler, Anne M; Caselli, Emilia; Prati, Fabio; Bonomo, Robert A

    2009-06-02

    Inhibitor-resistant class A beta-lactamases of the TEM and SHV families that arise by single amino acid substitutions are a significant threat to the efficacy of beta-lactam/beta-lactamase inhibitor combinations. To better understand the basis of the inhibitor-resistant phenotype in SHV, we performed mutagenesis to examine the role of a second-shell residue, Asn276. Of the 19 variants expressed in Escherichia coli, only the Asn276Asp enzyme demonstrated reduced susceptibility to ampicillin/clavulanate (MIC increased from 50/2 --> 50/8 microg/mL) while maintaining high-level resistance to ampicillin (MIC = 8192 microg/mL). Steady-state kinetic analyses of Asn276Asp revealed slightly diminished k(cat)/K(m) for all substrates tested. In contrast, we observed a 5-fold increase in K(i) for clavulanate (7.4 +/- 0.9 microM for Asn276Asp vs 1.4 +/- 0.2 microM for SHV-1) and a 40% reduction in k(inact)/K(I) (0.013 +/- 0.002 microM(-1 )s(-1) for Asn276Asp vs 0.021 +/- 0.004 microM(-1) s(-1) for SHV-1). Timed electrospray ionization mass spectrometry of clavulanate-inhibited SHV-1 and SHV Asn276Asp showed nearly identical mass adducts, arguing for a similar pathway of inactivation. Molecular modeling shows that novel electrostatic interactions are formed between Arg244Neta2 and both 276AspOdelta1 and Odelta2; these new forces restrict the spatial position of Arg244, a residue important in the recognition of the C(3)/C(4) carboxylate of beta-lactam substrates and inhibitors. Testing the functional consequences of this interaction, we noted considerable free energy costs (+DeltaDeltaG) for substrates and inhibitors. A rigid carbapenem (meropenem) was most affected by the Asn276Asp substitution (46-fold increase in K(i) vs SHV-1). We conclude that residue 276 is an important second-shell residue in class A beta-lactamase-mediated resistance to substrates and inhibitors, and only Asn is able to precisely modulate the conformational flexibility of Arg244 required for successful

  13. Review of declarations received by the AFSSAPS and the ASN on the account of radio-vigilance (external radiotherapy). July 2007 - June 2008

    International Nuclear Information System (INIS)

    2009-09-01

    After a presentation of the procedure of declaration of significant radiation protection events, of the ASN-SFRO event scale, and of the indication procedure related to material vigilance, this report proposes a review and an analysis of significant radioprotection and material vigilance events which have been declared between July 2007 and June 2008. The assessment of radiation protection events declared to the ASN is as well quantitative (distribution in time, geographical distribution, status of declaring centres, declaration delays, classification according to the ASN-SFRO scale) as qualitative (origin, event typology, stage of the treatment process at which the event occurred and has been detected, personnel having detected the event, communication about events). The analysis of material vigilance events addresses the evolution of their number, their distribution in terms of declarer type, of radiotherapy equipment, or of criticality level, and so on. A review of hybrid accidents is proposed (concerned equipment, declarer origin, consequences of the declarations, classification on the ASN-SFRO scale)

  14. Employee coaching and counseling program metode alternatif untuk optimalisasi human capital pada pegawai aparatur sipil negara (ASN

    Directory of Open Access Journals (Sweden)

    Marina Sulastiana

    2017-06-01

    Full Text Available State Civil Servants Apparatus (ASN have to be more integrity, professional, neutral and capable to carrying out a public service to the community. It was an important one that can be optimized through development of human capital. Based on previous research investigated role of Human Capital towards Services and performance of Public Service, shows that staff and head divison level only has moderate degree of human capital. While, Emotional Capital was a most dominan aspect in Human Capital. Otherwise, Social Capital aspects results in the low level. It comes with consequences to optimized them through psychological intervention with group Employee Coaching and Counseling Program (ECCP. This study was action research, experimental one group preteset and postest design. The result shows that ECCP was contribute effectively to enhance Human Capital, particularly in Emotional Capital optimization.

  15. NMR structure determination of a synthetic analogue of bacillomycin Lc reveals the strategic role of L-Asn1 in the natural iturinic antibiotics

    Science.gov (United States)

    Volpon, Laurent; Tsan, Pascale; Majer, Zsuzsa; Vass, Elemer; Hollósi, Miklós; Noguéra, Valérie; Lancelin, Jean-Marc; Besson, Françoise

    2007-08-01

    Iturins are a group of antifungal produced by Bacillus subtilis. All are cyclic lipopeptides with seven α-amino acids of configuration LDDLLDL and one β-amino fatty acid. The bacillomycin L is a member of this family and its NMR structure was previously resolved using the sequence Asp-Tyr-Asn-Ser-Gln-Ser-Thr. In this work, we carefully examined the NMR spectra of this compound and detected an error in the sequence. In fact, Asp1 and Gln5 need to be changed into Asn1 and Glu5, which therefore makes it identical to bacillomycin Lc. As a consequence, it now appears that all iturinic peptides with antibiotic activity share the common β-amino fatty acid 8- L-Asn1- D-Tyr2- D-Asn3 sequence. To better understand the conformational influence of the acidic residue L-Asp1, present, for example in the inactive iturin C, the NMR structure of the synthetic analogue SCP [cyclo ( L-Asp1- D-Tyr2- D-Asn3- L-Ser4- L-Gln5- D-Ser6- L-Thr7-β-Ala8)] was determined and compared with bacillomycin Lc recalculated with the corrected sequence. In both cases, the conformers obtained were separated into two families of similar energy which essentially differ in the number and type of turns. A detailed analysis of both cyclopeptide structures is presented here. In addition, CD and FTIR spectra were performed and confirmed the conformational differences observed by NMR between both cyclopeptides.

  16. ASN takes position in the in-service follow-up programs of primary and secondary loops of EdF's nuclear power plants

    International Nuclear Information System (INIS)

    2002-01-01

    This decision from the French authority of nuclear safety (ASN) aims at fixing the conditions to be respected by Electricite de France (EdF) during its in-service follow-up programs for the monitoring and preventive maintenance of the primary and secondary cooling loops of EdF's PWR reactors. The components and the particular points to be controlled are listed in appendixes. (J.S.)

  17. Water dynamics of Ser-His-Glu-Cys-Asn powder and effects of moisture absorption on its chemical properties.

    Science.gov (United States)

    Lin, Songyi; Xue, Peiyu; Yang, Shuailing; Li, Xingfang; Dong, Xiuping; Chen, Feng

    2017-08-01

    This study has elucidated moisture dynamics in the soybean peptide, Ser-His-Glu-Cys-Asn (SHECN) powder by using dynamic vapor sorption (DVS) and nuclear magnetic resonance (NMR). We also tried to investigate the effects of moisture absorption on the biological activity and chemical properties of SHECN with some effective methods such as mid-infrared (MIR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). DVS results showed that the moisture absorption of SHECN could reach a maximum of 33%, and the SHECN powder after synthesis actually existed in a trihydrate state of SHECN.3H 2 O. Low-field NMR revealed that three water proportions including strong combined water, binding water and bulk water were involved in SHECN moisture absorption and absored water dominantly existed in the form of combined water. Magnetic resonance imaging (MRI) and MIR spectroscopy results indicated that moisture absorption could change the morphology and structure of SHECN. After moisture absorption at 50% and 75% relative humidity, 19 volatiles were identified by GC-MS analysis. Additionally, this study showed that a part of reductive groups in SHECN was oxidized and its antioxidant ability declined significantly (P moisture absorption. Water absorbed into SHECN powder can significantly change its microstructure and cause its activity to decrease. We must prevent SHECN from absorbing moisture during storage because the water can accelerate the oxidation of samples and promote microbial reactions. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  18. Radioimmunoassay for Lys8, Asn9, neurotensin 8-13: tissue and subcellular distribution of immunoreactivity in chickens

    International Nuclear Information System (INIS)

    Carraway, R.E.; Ruane, S.E.; Ritsema, R.S.

    1983-01-01

    A sensitive and specific radioimmunoassay (RIA) for Lys8, Asn9, neurotensin 8-13 (LANT-6) has been developed which utilizes 125I-labeled LANT-6 and rabbit antisera raised towards conjugates of synthetic LANT-6 and bovine thyroglobulin. The antiserum described (TG-22) allows the detection of ca 100 fmol of LANT-6 and crossreacts less than 0.01% with chicken or bovine NT. Dose-response relationships for the native (chicken) and synthetic peptides were indistinguishable. Using this assay the distribution of immunoreactive LANT-6 (iLANT-6) through various tissues of the chicken was studied and compared with that of chicken NT (iNT) determined by RIA. Both iNT and ILANT-6 were found primarily in the brain and gastrointestinal tract, however, their regional distributions were found to differ. Subcellular distribution studies in homogenates of chicken brain indicated that both iNT and iLANT-6 were associated with synaptosome-like and vesicle-like particles. In homogenates of small intestine, pancreas and colon iNT and iLANT-6 appeared to be within osmotically sensitive, sedimentable particles. Analyses using high pressure liquid chromatography established that chicken iLANT-6 co-eluted with the synthetic peptide and that similar substances were present in extracts of rat brain and intestine. These results are consistent with ''messenger' roles for these peptides

  19. Lessons learned from events declared to the ASN related to interventional radiology and having occurred during radiation-based acts

    International Nuclear Information System (INIS)

    Lachaume, Jean-Luc

    2014-01-01

    Based on an analysis of events declared to the ASN and inspection observations performed in the field of interventional radiology, this report outlines that the majority of these events could have been avoided and that they result from a lack of culture in radiation protection, notably an unawareness of doses delivered to patients or received by practitioners, and of risks related to exposure to ionizing radiations. The report notably outlines that events are related to a lack of staff and means in the field of patient and personnel radiation protection, an underdeveloped risk management and radiation protection implementation, lacks in the management of delivered or received doses and absence of approaches of professional practice assessment, operator insufficient education, and weaknesses in the management of subcontracted operations. Recommendations are made related to needs in medical radio-physics, identification of acts and patients at risk and definition of patient follow-up modalities, the implementation of an approach of professional practice assessment, the storage of dosimetric data, the improvement of operator technical education, the control of subcontracted operations, and the anticipation of technical and organisational changes

  20. Flexible xxx-asp/asn and gly-xxx residues of equine cytochrome C in matrix-assisted laser desorption/ionization in-source decay mass spectrometry.

    Science.gov (United States)

    Takayama, Mitsuo

    2012-01-01

    The backbone flexibility of a protein has been studied from the standpoint of the susceptibility of amino acid residues to in-source decay (ISD) in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Residues more susceptible to MALDI-ISD, namely Xxx-Asp/Asn and Gly-Xxx, were identified from the discontinuous intense peak of c'-ions originating from specific cleavage at N-Cα bonds of the backbone of equine cytochrome c. The identity of the residues susceptible to ISD was consistent with the known flexible backbone amides as estimated by hydrogen/deuterium exchange (HDX) experiments. The identity of these flexible amino acid residues (Asp, Asn, and Gly) is consistent with the fact that these residues are preferred in flexible secondary structure free from intramolecular hydrogen-bonded structures such as α-helix and β-sheet. The MALDI-ISD spectrum of equine cytochrome c gave not only intense N-terminal side c'-ions originating from N-Cα bond cleavage at Xxx-Asp/Asn and Gly-Xxx residues, but also C-terminal side complement z'-ions originating from the same cleavage sites. The present study implies that MALDI-ISD can give information about backbone flexibility of proteins, comparable with the protection factors estimated by HDX.

  1. Flexible Xxx–Asp/Asn and Gly–Xxx Residues of Equine Cytochrome c in Matrix-Assisted Laser Desorption/Ionization In-Source Decay Mass Spectrometry

    Science.gov (United States)

    Takayama, Mitsuo

    2012-01-01

    The backbone flexibility of a protein has been studied from the standpoint of the susceptibility of amino acid residues to in-source decay (ISD) in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Residues more susceptible to MALDI-ISD, namely Xxx–Asp/Asn and Gly–Xxx, were identified from the discontinuous intense peak of c′-ions originating from specific cleavage at N–Cα bonds of the backbone of equine cytochrome c. The identity of the residues susceptible to ISD was consistent with the known flexible backbone amides as estimated by hydrogen/deuterium exchange (HDX) experiments. The identity of these flexible amino acid residues (Asp, Asn, and Gly) is consistent with the fact that these residues are preferred in flexible secondary structure free from intramolecular hydrogen-bonded structures such as α-helix and β-sheet. The MALDI-ISD spectrum of equine cytochrome c gave not only intense N-terminal side c′-ions originating from N–Cα bond cleavage at Xxx–Asp/Asn and Gly–Xxx residues, but also C-terminal side complement z′-ions originating from the same cleavage sites. The present study implies that MALDI-ISD can give information about backbone flexibility of proteins, comparable with the protection factors estimated by HDX. PMID:24349908

  2. Stěžejní východiska a koncepce současné anglické náboženské pedagogiky

    Directory of Open Access Journals (Sweden)

    Karim Sidibe

    2017-08-01

    Full Text Available Tento článek si klade za cíl analyzovat didaktické koncepce výuky náboženství pěti hlavních teoretiků současné anglické náboženské pedagogiky, Harolda Loukese, Niniana Smarta, Davida Haye, Cliva Errickera a Andrewa Wrighta. Loukes a Smart položili základy současné náboženské pedagogiky v Anglii v 60. letech 20. století. Loukes využil poznatky vývojové psychologie a Smart výuku sekularizoval. Hayovo pojetí z 80. let 20. století akcentuje niternou duchovní skutečnost žáků. V současné době Erricker aplikuje postmoderní filosofická východiska na výuku a akcentuje individualitu a nezávislost žáků, zatímco Wright vychází z filosofických východisek kritického realismu a akcentuje nutnost teologické a filosofické diskuze na hodinách náboženství.

  3. Biologically active and C-amidated hinnavinII-38-Asn produced from a Trx fusion construct in Escherichia coli.

    Science.gov (United States)

    Kang, Chang Soo; Son, Seung-Yeol; Bang, In Seok

    2008-12-01

    The cabbage butterfly (Artogeia rapae) antimicrobial peptide hinnavinII as a member of cecropin family is synthesized as 37 residues in size with an amidated lysine at C-terminus and shows the humoral immune response to a bacterial invasion. In this work, a synthetic gene for hinnavinII-38-Asn (HIN) with an additional amino acid asparagine residue containing amide group at C-terminus was cloned into pET-32a(+) vector to allow expression of HIN as a Trx fusion protein in Escherichia coli strain BL21 (DE3) pLysS. The resulting expression level of the fusion protein Trx-HIN could reach 15-20% of the total cell proteins and more than 70% of the target proteins were in soluble form. The fusion protein could be purified successfully by HiTrap Chelating HP column and a high yield of 15 mg purified fusion protein was obtained from 80 ml E. coli culture. Recombinant HIN was readily obtained by enterokinase cleavage of the fusion protein followed by FPLC chromatography, and 3.18 mg pure active recombinant HIN was obtained from 80 ml culture. The molecular mass of recombinant HIN determined by MALDI-TOF mass spectrometer is 4252.084 Da which matches the theoretical mass (4252.0 Da) of HIN. Comparing the antimicrobial activities of the recombinant hinnavinII with C-amidated terminus to that without an amidated C-terminus, we found that the amide of asparagine at C-terminus of hinnavinII improved its potency on certain microorganism such as E. coli, Enterobacter cloacae, Bacillus megaterium, and Staphylococcus aureus.

  4. Especificação e Implementação de Templates GDMO e Tipos ASN 1 para Linguagem C++

    OpenAIRE

    Junior, Rivalino Matias; Specialski, Elizabeth Sueli; May, Karina; Ropelato, Patricia

    1996-01-01

    Este artigo descreve a especificação e implementação de regras para o mapeamento de templates GDMO (GuideUnes lor the Definition 01 Managed Objects) [1 l, além da implementação dos tipos ASN.1, em linguagem de programação C++[2l. Estas regras serao utilizadas no mapeamentotemplate - GDMO para classes (dass) em C++, linguagem utilizada em todo o projeto da Plataforma de Gerencia da Rede Local UFSC, a qual segue o modelo de gerenciamento de redes OSI.

  5. Asn563Ser polymorphism of CD31/PECAM-1 is associated with atherosclerotic cerebral infarction in a southern Han population

    Directory of Open Access Journals (Sweden)

    Song YM

    2014-12-01

    Full Text Available Yanmin Song, Qunfang Li, Lili Long, Ning Zhang, Yunhai Liu Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan Province, People’s Republic of China Background: CD31, also called platelet endothelial cell adhesion molecule-1 (PECAM-1, is thought to play a role in the pathological mechanisms of atherosclerosis. Leu125Val polymorphism and elevated plasma levels of soluble PECAM-1 (sPECAM-1 were found to be associated with cerebral infarction. Our aim was to investigate the association between the Asn563Ser polymorphism of CD31/PECAM-1, plasma level of sPECAM-1, and the risk of atherosclerotic cerebral infarction (ACI in the southern Han population of the People’s Republic of China.Subjects and methods: A total of 147 subjects with ACI and 114 controls were enrolled in the study. The Asn563Ser CD31/PECAM-1 polymorphism was detected using the polymerase chain reaction–restriction fragment length polymorphism method. The plasma spECAM-1 level was measured using the enzyme-linked immunosorbent assay method.Results: In this study, statistically significant differences in Asn563Ser genotype and allele distribution were found between the cases and controls (P<0.05. Furthermore, logistic regression analysis showed that the GG genotype is associated with increase in ACI risk (odds ratio =4.862, P<0.001. The plasma level of sPECAM-1 was associated with ACI (odds ratio =1.431, P=0.038. In both the ACI and the control groups, the plasma sPECAM-1 level in subjects with the GG genotype was higher than that in subjects carrying the AA or GA genotype (P<0.05.Conclusion: Our study showed that the Asn563Ser polymorphism of CD31/PECAM-1 gene and elevated plasma sPECAM-1 level are related to ACI risk in the southern Han population of People’s Republic of China. Keywords: genetic polymorphism, CD31, platelet endothelial cell adhesion molecule-1 (PECAM-1

  6. A New Ala-122-Asn Amino Acid Change Confers Decreased Fitness to ALS-Resistant Echinochloa crus-galli

    Directory of Open Access Journals (Sweden)

    Silvia Panozzo

    2017-11-01

    Full Text Available Gene mutations conferring herbicide resistance may cause pleiotropic effects on plant fitness. Knowledge of these effects is important for managing the evolution of herbicide-resistant weeds. An Echinochloa crus-galli population resistant to acetolactate synthase (ALS herbicides was collected in a maize field in north-eastern Italy and the cross-resistance pattern, resistance mechanism and fitness costs associated to mutant-resistant plants under field conditions in the presence or absence of intra-specific competition were determined. The study reports for the first time the Ala-122-Asn amino-acid change in the ALS gene that confers high levels of cross-resistance to all ALS inhibitors tested. Results of 3-year growth analysis showed that mutant resistant E. crus-galli plants had a delayed development in comparison with susceptible plants and this was registered in both competitive (3, 7, and 20 plants m-2 and non-competitive (spaced plants situations. The number of panicles produced by resistant plants was also lower (about 40% fewer panicles than susceptible plants under no-intraspecific competition. Instead, with the increasing competition level, the difference in panicle production at harvest time decreased until it became negligible at 20 plants m-2. Evaluation of total dry biomass as well as biomass allocation in vegetative parts did not highlight any difference between resistant and susceptible plants. Instead, panicle dry weight was higher in susceptible plants indicating that they allocated more biomass than resistant ones to the reproductive organs, especially in no-competition and in competition situations at lower plant densities. The different fitness between resistant and susceptible phenotypes suggests that keeping the infestation density as low as possible can increase the reproduction success of the susceptible phenotype and therefore contribute to lowering the ratio between resistant and susceptible alleles. If adequately

  7. A New Ala-122-Asn Amino Acid Change Confers Decreased Fitness to ALS-Resistant Echinochloa crus-galli.

    Science.gov (United States)

    Panozzo, Silvia; Scarabel, Laura; Rosan, Valentina; Sattin, Maurizio

    2017-01-01

    Gene mutations conferring herbicide resistance may cause pleiotropic effects on plant fitness. Knowledge of these effects is important for managing the evolution of herbicide-resistant weeds. An Echinochloa crus-galli population resistant to acetolactate synthase (ALS) herbicides was collected in a maize field in north-eastern Italy and the cross-resistance pattern, resistance mechanism and fitness costs associated to mutant-resistant plants under field conditions in the presence or absence of intra-specific competition were determined. The study reports for the first time the Ala-122-Asn amino-acid change in the ALS gene that confers high levels of cross-resistance to all ALS inhibitors tested. Results of 3-year growth analysis showed that mutant resistant E. crus-galli plants had a delayed development in comparison with susceptible plants and this was registered in both competitive (3, 7, and 20 plants m -2 ) and non-competitive (spaced plants) situations. The number of panicles produced by resistant plants was also lower (about 40% fewer panicles) than susceptible plants under no-intraspecific competition. Instead, with the increasing competition level, the difference in panicle production at harvest time decreased until it became negligible at 20 plants m -2 . Evaluation of total dry biomass as well as biomass allocation in vegetative parts did not highlight any difference between resistant and susceptible plants. Instead, panicle dry weight was higher in susceptible plants indicating that they allocated more biomass than resistant ones to the reproductive organs, especially in no-competition and in competition situations at lower plant densities. The different fitness between resistant and susceptible phenotypes suggests that keeping the infestation density as low as possible can increase the reproduction success of the susceptible phenotype and therefore contribute to lowering the ratio between resistant and susceptible alleles. If adequately embedded in a

  8. Probing the diphosphoglycerate binding pocket of HbA and HbPresbyterian (beta 108Asn --> Lys).

    Science.gov (United States)

    Gottfried, D S; Manjula, B N; Malavalli, A; Acharya, A S; Friedman, J M

    1999-08-31

    HbPresbyterian (beta 108Asn --> Lys, HbP) contains an additional positive charge (per alpha beta dimer) in the middle of the central cavity and exhibits a lower oxygen affinity than wild-type HbA in the presence of chloride. However, very little is known about the molecular origins of its altered functional properties. In this study, we have focused on the beta beta cleft of the Hb tetramer. Recently, we developed an approach for quantifying the ligand binding affinity to the beta-end of the Hb central cavity using fluorescent analogues of the natural allosteric effector 2, 3-diphosphoglycerate (DPG) [Gottfried, D. S., et al. (1997) J. Biol. Chem. 272, 1571-1578]. Time-correlated single-photon counting fluorescence lifetime studies were used to assess the binding of pyrenetetrasulfonate to both HbA and HbP in the deoxy and CO ligation states under acidic and neutral pH conditions. Both the native and mutant proteins bind the probe at a weak binding site and a strong binding site; in all cases, the binding to HbP was stronger than to HbA. The most striking finding was that for HbA the binding affinity varies as follows: deoxy (pH 6.35) > deoxy (pH 7.20) > CO (pH 6.35); however, the binding to HbP is independent of ligation or pH. The mutant oxy protein also hydrolyzes p-nitrophenyl acetate, through a reversible acyl-imidazole pathway linked to the His residues of the beta beta cleft, at a considerably higher rate than does HbA. This implies a perturbation of the microenvironment of these residues at the DPG binding pocket. Structural consequences due to the presence of the new positive charge in the middle of the central cavity have been transmitted to the beta beta cleft of the protein, even in its liganded conformation. This is consistent with a newly described quaternary state (B) for liganded HbPresbyterian and an associated change in the allosteric control mechanism.

  9. The national plan of radioactive materials and wastes management. ASN's notice about the choice of the sites to be investigated in view of the setting up of a disposal facility for low activity/long living wastes

    International Nuclear Information System (INIS)

    2009-07-01

    This short presentation, given by the national safety authority (ASN) at the meeting of July 1, 2009 of the high committee for the nuclear safety transparency and information (HCTISN), presents, first, the second French national plan of radioactive materials and wastes management (PNGMDR) with its main priorities, and then, the choice of the sites to survey in view of the setting up of a disposal facility for low level/long living wastes. The ASN expresses its opinion about the criteria retained by the ANDRA (the national agency of radioactive waste management) to select the most suitable sites. (J.S.)

  10. Regulation Mechanism of the ald Gene Encoding Alanine Dehydrogenase in Mycobacterium smegmatis and Mycobacterium tuberculosis by the Lrp/AsnC Family Regulator AldR.

    Science.gov (United States)

    Jeong, Ji-A; Hyun, Jaekyung; Oh, Jeong-Il

    2015-10-01

    In the presence of alanine, AldR, which belongs to the Lrp/AsnC family of transcriptional regulators and regulates ald encoding alanine dehydrogenase in Mycobacterium smegmatis, changes its quaternary structure from a homodimer to an octamer with an open-ring conformation. Four AldR-binding sites (O2, O1, O4, and O3) with a consensus sequence of GA/T-N2-NWW/WWN-N2-A/TC were identified upstream of the M. smegmatis ald gene by means of DNase I footprinting analysis. O2, O1, and O4 are required for the induction of ald expression by alanine, while O3 is directly involved in the repression of ald expression. In addition to O3, both O1 and O4 are also necessary for full repression of ald expression in the absence of alanine, due to cooperative binding of AldR dimers to O1, O4, and O3. Binding of a molecule of the AldR octamer to the ald control region was demonstrated to require two AldR-binding sites separated by three helical turns between their centers and one additional binding site that is in phase with the two AldR-binding sites. The cooperative binding of AldR dimers to DNA requires three AldR-binding sites that are aligned with a periodicity of three helical turns. The aldR gene is negatively autoregulated independently of alanine. Comparative analysis of ald expression of M. smegmatis and Mycobacterium tuberculosis in conjunction with sequence analysis of both ald control regions led us to suggest that the expression of the ald genes in both mycobacterial species is regulated by the same mechanism. In mycobacteria, alanine dehydrogenase (Ald) is the enzyme required both to utilize alanine as a nitrogen source and to grow under hypoxic conditions by maintaining the redox state of the NADH/NAD(+) pool. Expression of the ald gene was reported to be regulated by the AldR regulator that belongs to the Lrp/AsnC (feast/famine) family, but the underlying mechanism was unknown. This study revealed the regulation mechanism of ald in Mycobacterium smegmatis and

  11. Improving radiotherapy quality and safety: a common objective of A.S.N. and the Ile de France Regional Hospital Agency. Thoughts on successful cooperation in Ile de France

    International Nuclear Information System (INIS)

    Solier, N.

    2009-01-01

    Radiotherapy recent development in cancer treatment needs strong procedures and quality assessment to improve safety both for patients and professionals. In Ile de France territory, a coordinated action between ASN inspectors and medical doctors specialized in public health (MISP), working as civil servants in health administration, was initiated at the end of 2007. An inspection guideline was implemented in a workshop between ASN and Regional Hospital Agency (ARH). The MISP as health administrator has to verify if care organisation responds to treatment cancer regulatory framework: as a doctor he is authorized to access to patient records to know which treatment was chosen. He has to assure if the therapeutic guidelines are respected. His comprehensive approach is essentially patient-oriented. The MISP inspection coordinated with ASN inspectors is a very pertinent pluri-disciplinary approach, undeniably necessary in such a medical technic. The effectiveness of the radiotherapy pluri-disciplinary team is funded on sharing procedures and experiences, respecting different knowledge and practices in daily work. This on site audit'. seems to be very fertile both for the radiotherapy team, the MISP and ASN inspectors. Continuous quality improvement in radiotherapy in the Ile de France region is a fertile issue between ANS and ARH teams. Confronting knowledge and procedures during the 'on site audit' in a transparent way will contribute improving radiotherapy for the 30.000 Francilian patients which receive this treatment each year. This challenge is our main concern to facilitate the patients access to the best standards treatments. (author)

  12. Hematological and Molecular Characterization of Hb J-Buda [α61(E10)Lys → Asn, AAG > AAT].

    Science.gov (United States)

    Panyasai, Sitthichai; Permsripong, Nopphadol; Jaiping, Kanokwan; Khantarag, Pisuttinee; Pornprasert, Sakorn

    2016-06-01

    Hemoglobin (Hb) J-Buda [α61(E10)Lys → Asn, AAG > AAT] is a very rare α-chain variant found in South-East Asia. We analyzed hematological parameters and provided a rapid molecular analysis method for detection of this hemoglobinopathy in two Thai women who had severe microcytic anemia with Hb and MCV AAG → AAT mutation at codon 61 for Hb J-Buda on one allele of the α1-globin gene. The developed Allele-specific PCR (ASPCR) showed the 455 bp amplified fragment from Hb J-Buda allele. Thus, understanding of hematological characterizations and the developed ASPCR for diagnosis of Hb J-Buda are essential for genetic counseling of this hemoglobinopathy.

  13. Substrate recognition and catalysis by GH47 α-mannosidases involved in Asn-linked glycan maturation in the mammalian secretory pathway

    Energy Technology Data Exchange (ETDEWEB)

    Xiang, Yong; Karaveg, Khanita; Moremen, Kelley W.

    2016-11-17

    Asn-linked glycosylation of newly synthesized polypeptides occurs in the endoplasmic reticulum of eukaryotic cells. Glycan structures are trimmed and remodeled as they transit the secretory pathway, and processing intermediates play various roles as ligands for folding chaperones and signals for quality control and intracellular transport. Key steps for the generation of these trimmed intermediates are catalyzed by glycoside hydrolase family 47 (GH47) α-mannosidases that selectively cleave α1,2-linked mannose residues. Despite the sequence and structural similarities among the GH47 enzymes, the molecular basis for residue-specific cleavage remains obscure. The present studies reveal enzyme–substrate complex structures for two related GH47 α-mannosidases and provide insights into how these enzymes recognize the same substrates differently and catalyze the complementary glycan trimming reactions necessary for glycan maturation.

  14. Control of doses delivered to patients in medical imagery. Progress report of the program of actions recommended by the ASN. January 2015

    International Nuclear Information System (INIS)

    2015-01-01

    This report proposes an overview and an assessment of the various actions recommended by the ASN (the French Nuclear Safety Authority) for a better management of doses delivered to patients in the case of medical imagery. It indicates and discusses the progress noticed: actions which have been already performed, actions which are currently in progress, and actions which are still to be launched. These actions concern various aspects: the practice quality and safety (quality and safety management), the organisation, human resources and training, equipment and installation safety, radio-vigilance practices, relationships with patients, knowledge of practices and of exposures of patients and workers. Some highlights are outlined: some persistent lacks in human resources, some encouraging initiatives in the field of training, some progresses in the field of practice quality and safety, and some limited results regarding equipment (notably MRI) and installation safety

  15. Summary of the report A.S.N. n. 2006 ENSTR 019 - IGAS n. RM 2007-015P on the Epinal radiotherapy accident

    International Nuclear Information System (INIS)

    2007-01-01

    The Nuclear Safety Authority (A.S.N.) and the General Inspection of Social Affairs (I.G.A.S.) have been in charge by the Ministry of an inquiry on the accident that has lead to the overdose of 23 patients irradiated for a prostate cancer at the Hospital of Epinal. This event constitutes the most important accident implying the ionizing radiations occurring in France. In may 2004, the protocol of conformal radiotherapy applied to the prostate cancers has been modified, it uses dynamics corners in place of static corners. This change supposes to modify the parameters definition that gives the calculation of irradiation intensity, that will not not be made for some of patients. This mistake could be changed if the in vivo dosimetry that allows to check the real dose received by the patients had been kept. but this line of safety has been removed, the use of dynamic corners making it ineffective. The period of over irradiation has been from the 6. may 2004 to the 1. august 2005. During the summer 2005, the radiotherapist and radio physicist find the overdose error by examining the patients files again. On the 15. september 2005, the two doctors of the service warn the director of the C.H.J.M. (hospital center of Jean Monnet) of the accident that informs D.D.A.S.S. (departmental direction of sanitary and social affairs) and A.R.H. (regional agencies of hospitalization). But the national authorities (A.F.S.S.A.P.S., A.S.N., I.R.S.N.) have not been warned, and so nobody can recognize the unsuitable character of the decided measures. Only seven patients are informed of the overdose of radiations they received during the last trimester 2005. The assistance to the victims is not organised the first year. All the patients have received usual treatments when they should receive a treatment by graft of mesenchymatous stem cells. Between september 2005 and september 2006 four patients died and ten present serious complications. propositions have been made to help the victims and

  16. Elimination of effluents and wastes contaminated by radionuclides produced in installations authorized on the account of the Public Health Code - ASN guide nr 18 - Release of the 26/01/2012

    International Nuclear Information System (INIS)

    2012-01-01

    This document aims at specifying the modalities of application of a decision taken by the ASN in January 2008 regarding the technical rules which the elimination of effluents and wastes contaminated by radionuclides must comply with. First, it describes the objective of the 'waste zoning' concept. Then, it addresses the management of contaminated wastes: general rules, wastes contaminated by radionuclides of very short period (less than 100 days) and of period longer than 100 days, and the management of wastes with 'hybrid risks'. It addresses the management of contaminated effluents: radioactive liquid effluent with a period either shorter or longer than 100 days, radioactive gaseous effluents. It addresses the warehousing conditions (premise design, exploitation rules). The other parts deal with the convention between several establishments within a same site (notably in the case of nuclear medicine departments), with the management plan, and with the agenda for the implementation of the ASN decision

  17. Structure-activity relationships of the unique and potent agouti-related protein (AGRP)-melanocortin chimeric Tyr-c[beta-Asp-His-DPhe-Arg-Trp-Asn-Ala-Phe-Dpr]-Tyr-NH2 peptide template.

    Science.gov (United States)

    Wilczynski, Andrzej; Wilson, Krista R; Scott, Joseph W; Edison, Arthur S; Haskell-Luevano, Carrie

    2005-04-21

    The melanocortin receptor system consists of endogenous agonists, antagonists, G-protein coupled receptors, and auxiliary proteins that are involved in the regulation of complex physiological functions such as energy and weight homeostasis, feeding behavior, inflammation, sexual function, pigmentation, and exocrine gland function. Herein, we report the structure-activity relationship (SAR) of a new chimeric hAGRP-melanocortin agonist peptide template Tyr-c[beta-Asp-His-DPhe-Arg-Trp-Asn-Ala-Phe-Dpr]-Tyr-NH(2) that was characterized using amino acids previously reported in other melanocortin agonist templates. Twenty peptides were examined in this study, and six peptides were selected for (1)H NMR and computer-assisted molecular modeling structural analysis. The most notable results include the identification that modification of the chimeric template at the His position with Pro and Phe resulted in ligands that were nM mouse melanocortin-3 receptor (mMC3R) antagonists and nM mouse melanocortin-4 receptor (mMC4R) agonists. The peptides Tyr-c[beta-Asp-His-DPhe-Ala-Trp-Asn-Ala-Phe-Dpr]-Tyr-NH(2) and Tyr-c[beta-Asp-His-DNal(1')-Arg-Trp-Asn-Ala-Phe-Dpr]-Tyr-NH(2) resulted in 730- and 560-fold, respectively, mMC4R versus mMC3R selective agonists that also possessed nM agonist potency at the mMC1R and mMC5R. Structural studies identified a reverse turn occurring in the His-DPhe-Arg-Trp domain, with subtle differences observed that may account for the differences in melanocortin receptor pharmacology. Specifically, a gamma-turn secondary structure involving the DPhe(4) in the central position of the Tyr-c[beta-Asp-Phe-DPhe-Arg-Trp-Asn-Ala-Phe-Dpr]-Tyr-NH(2) peptide may differentiate the mixed mMC3R antagonist and mMC4R agonist pharmacology.

  18. "Minulost je bitevním polem současníků". Konference k osmdesátinám Viléma Prečana

    Czech Academy of Sciences Publication Activity Database

    Hoppe, Jiří

    2013-01-01

    Roč. 20, 1/2 (2013), s. 236-241 ISSN 1210-7050. ["Minulost je bitevním polem současníků". Konference k osmdesátinám Viléma Prečana. Praha, 24.01.2013-25.01.2013] Institutional support: RVO:68378114 Keywords : Vilém Prečan Subject RIV: AB - History

  19. Celostátní seminář Celoživotní učení - Kroměříž 2006 "Současná realita - budoucnost".

    Czech Academy of Sciences Publication Activity Database

    Basl, Josef

    2006-01-01

    Roč. 10, č. 6 (2006) ISSN 1212-5075. [Celoživotní učení - Kroměříž 2006 "Současná realita - budoucnost". Kroměříž, 18.05.2006-19.05.2006] R&D Projects: GA ČR GA403/06/1421 Institutional research plan: CEZ:AV0Z70280505 Keywords : Czech Republic * lifelong learning * current issues Subject RIV: AO - Sociology, Demography

  20. Antioxidant and antiglycation activities of the synthesised dipeptide, Asn-Trp, derived from computer-aided simulation of yam dioscorin hydrolysis and its analogue, Gln-Trp.

    Science.gov (United States)

    Han, Chuan-Hsiao; Lin, Yin-Shiou; Lin, Shyr-Yi; Hou, Wen-Chi

    2014-03-15

    Previous studies have shown that the Trp residue contributes to a high oxygen radical absorbance capacity (ORAC). Therefore, in this study, a Trp-containing dipeptide derived from a computer-aided simulation of pepsin hydrolysis of the yam tuber, dioscorin-namely, Asn-Trp (NW), and its analogue, Gln-Trp (QW)-were synthesised to compare their antioxidant and antiglycation activities with carnosine, homocarnosine, or glutathione (GSH). The antioxidant assays included hydroxyl radical-scavenging activity, anti-AAPH-induced hemolysis, and ORAC activity. NW had a significantly higher antioxidant activity than had QW and performed much better than carnosine, homocarnosine, or GSH. Using bovine serum albumin (BSA)/galactose or BSA/glucose as experimental models, NW had better antiglycation effects than had QW, as detected by an anti-N(ε)-(carboxymethyl)lysine (anti-CML) antibody. Moreover, NW and QW (50-200 μM) showed protection against methylglyoxal-induced cell deaths in human umbilical vein endothelial cells. These results suggest that NW, derived from computer-aided simulation of dioscorin hydrolysis, exhibits antioxidant and antiglycation activities, which thus shows the benefits of the yam tuber as an antioxidant-rich food. Copyright © 2013 Elsevier Ltd. All rights reserved.

  1. [Hb Burgos (α1 CD64(E13)(Asp→Asn)): a new hemoglobin variant detected during follow-up of diabetic patients].

    Science.gov (United States)

    de la Fuente-Gonzalo, Félix; Martínez Nieto, Jorge; Torrejón, María José; Mayor, Luis Antonio; Velasco, Diego; González Fernández, Fernando Ataulfo; Ropero Gradilla, Paloma

    2015-01-06

    The glycated hemoglobin (HbA1c) test by high performance liquid chromatography is a useful tool for the follow-up of diabetes mellitus patients. Some structural hemoglobin (Hb) variants are known to cause interference in the analytical measurement of HbA1c. In this study, it has been characterized a new Hb variant in 4 patients during their regular control of HbA1c. Selective α1 gene sequencing showed a mutation GAC>AAC at codon 64 within exon 2. This produces a change of aspartic acid (Asp) by asparagine (Asn) that does not produce any functional alteration so the resultant molecule behaves as a silent hemoglobinopathy. The structural Hb variants can be detected during the analysis of HbA1c and may alter its values. Though rare, this occurrence signals the need to being aware when measuring HbA1c. Copyright © 2014 Elsevier España, S.L.U. All rights reserved.

  2. Identification of homozygous WFS1 mutations (p.Asp211Asn, p.Gln486*) causing severe Wolfram syndrome and first report of male fertility.

    Science.gov (United States)

    Haghighi, Amirreza; Haghighi, Alireza; Setoodeh, Aria; Saleh-Gohari, Nasrollah; Astuti, Dewi; Barrett, Timothy G

    2013-03-01

    Wolfram syndrome (WFS) is a neurodegenerative genetic condition characterized by juvenile-onset of diabetes mellitus and optic atrophy. We studied clinical features and the molecular basis of severe WFS (neurodegenerative complications) in two consanguineous families from Iran. A clinical and molecular genetic investigation was performed in the affected and healthy members of two families. The clinical diagnosis of WFS was confirmed by the existence of diabetes mellitus and optic atrophy in the affected patients, who in addition had severe neurodegenerative complications. Sequencing of WFS1 was undertaken in one affected member from each family. Targeted mutations were tested in all members of relevant families. Patients had most of the reported features of WFS. Two affected males in the first family had fathered unaffected children. We identified two homozygous mutations previously reported with apparently milder phenotypes: family 1: c.631G>A (p.Asp211Asn) in exon 5, and family 2: c.1456C>T (p.Gln486*) in exon 8. Heterozygous carriers were unaffected. This is the first report of male Wolfram patients who have successfully fathered children. Surprisingly, they also had almost all the complications associated with WFS. Our report has implications for genetic counseling and family planning advice for other affected families.

  3. The functional polymorphism rs73598374:G>A (p.Asp8Asn) of the ADA gene is associated with telomerase activity and leukocyte telomere length.

    Science.gov (United States)

    Concetti, Fabio; Carpi, Francesco M; Nabissi, Massimo; Picciolini, Matteo; Santoni, Giorgio; Napolioni, Valerio

    2015-02-01

    Recent evidence demonstrated a relevant role of adenosine deaminase (ADA) in replicative senescence of T cells through its capacity to modulate telomerase activity (TA). Herein, we tested the impact of the functional polymorphism ADA rs73598374:G>A (c.22G>A, p.Asp8Asn) on telomere biology, by measuring TA and leukocyte telomere length (LTL) in healthy subjects selected according to rs73598374 genotype. rs73598374-A carriers showed lower TA (P=0.019) and shorter LTL (P=0.003), respectively, compared to G/G carriers. rs73598374-A carriers showed a stronger cross-sectional age reduction of LTL (r=-0.314, P=0.005) compared to G/G carriers (r=-0.243, P=0.022). The reduced ADA activity associated to rs73598374-A variant predisposes those carriers to display higher levels of adenosine compared to G/G carriers. Consequently, it may lead to an accelerated process of replicative senescence, causing a stronger reduction of TA and in turn shorter LTL. In conclusion, the crucial role played by replicative senescence of the immune system in several human diseases and in the aging process underscores the relevance of the present findings and also spurs interest into the possible involvement of rs73598374 in shaping the susceptibility to several age-related diseases.

  4. Protein changes associated with reprotonation of the Schiff base in the photocycle of Asp96-->Asn bacteriorhodopsin. The MN intermediate with unprotonated Schiff base but N-like protein structure

    Science.gov (United States)

    Sasaki, J.; Shichida, Y.; Lanyi, J. K.; Maeda, A.

    1992-01-01

    The difference Fourier transform infrared spectrum for the N intermediate in the photoreaction of the light-adapted form of bacteriorhodopsin can be recorded at pH 10 at 274 K (Pfefferle, J.-M., Maeda, A., Sasaki, J., and Yoshizawa, T. (1991) Biochemistry 30, 6548-6556). Under these conditions, Asp96-->Asn bacteriorhodopsin gives a photoproduct which shows changes in protein structure similar to those observed in N of wild-type bacteriorhodopsin. However, decreased intensity of the chromophore bands and the single absorbance maximum at about 400 nm indicate that the Schiff base is unprotonated, as in the M intermediate. This photoproduct was named MN. At pH 7, where the supply of proton is not as restricted as at pH 10, Asp96-->Asn bacteriorhodopsin yields N with a protonated Schiff base. The Asn96 residue, which cannot deprotonate as Asp96 in wild-type bacteriorhodopsin, is perturbed upon formation of both MN at pH 10 and N at pH 7. We suggest that the reprotonation of the Schiff base is preceded by a large change in the protein structure including perturbation of the residue at position 96.

  5. Purification, crystallization and preliminary X-ray crystallographic analysis of ST1022, a putative member of the Lrp/AsnC family of transcriptional regulators isolated from Sulfolobus tokodaii strain 7

    Energy Technology Data Exchange (ETDEWEB)

    Nakano, Noboru; Kumarevel, Thirumananseri, E-mail: tskvel@spring8.or.jp; Matsunaga, Emiko; Shinkai, Akeo [RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan); Kuramitsu, Seiki [RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan); Department of Biological Sciences, Graduate School of Science, Osaka University, Tayonaka, Osaka 560-0043 (Japan); Yokoyama, Shigeyuki, E-mail: tskvel@spring8.or.jp [RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan); Genomic Sciences Center, Yokohama Institute, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045 (Japan); Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan)

    2007-11-01

    A putative member of the Lrp/AsnC family of transcriptional regulators, ST1022 from S. tokodaii strain 7, has been purified and crystallized in the absence and presence of the effector l-glutamine. A molecular-replacement solution was found using the FL11 transcriptional regulator from Pyrococcus sp. OT3 as a model and structural refinement is under way. The Lrp/AsnC family of transcriptional regulators, also known as feast/famine transcriptional regulators, are widely distributed among bacteria and archaea. This family of proteins are likely to be involved in cellular metabolism, with exogenous amino acids functioning as effectors. Here, the crystallization and preliminary X-ray diffraction analysis of ST1022, a member of the Lrp/AsnC family of proteins, is reported with and without exogenous glutamine as the effector molecule. The crystals of native ST1022 and of the putative complex belong to the tetragonal space group I422, with unit-cell parameters a = b = 103.771, c = 73.297 Å and a = b = 103.846, c = 73.992 Å, respectively. Preliminary X-ray diffraction data analysis and molecular-replacement solution revealed the presence of one monomer per asymmetric unit.

  6. Purification, crystallization and preliminary X-ray crystallographic analysis of ST1022, a putative member of the Lrp/AsnC family of transcriptional regulators isolated from Sulfolobus tokodaii strain 7

    International Nuclear Information System (INIS)

    Nakano, Noboru; Kumarevel, Thirumananseri; Matsunaga, Emiko; Shinkai, Akeo; Kuramitsu, Seiki; Yokoyama, Shigeyuki

    2007-01-01

    A putative member of the Lrp/AsnC family of transcriptional regulators, ST1022 from S. tokodaii strain 7, has been purified and crystallized in the absence and presence of the effector l-glutamine. A molecular-replacement solution was found using the FL11 transcriptional regulator from Pyrococcus sp. OT3 as a model and structural refinement is under way. The Lrp/AsnC family of transcriptional regulators, also known as feast/famine transcriptional regulators, are widely distributed among bacteria and archaea. This family of proteins are likely to be involved in cellular metabolism, with exogenous amino acids functioning as effectors. Here, the crystallization and preliminary X-ray diffraction analysis of ST1022, a member of the Lrp/AsnC family of proteins, is reported with and without exogenous glutamine as the effector molecule. The crystals of native ST1022 and of the putative complex belong to the tetragonal space group I422, with unit-cell parameters a = b = 103.771, c = 73.297 Å and a = b = 103.846, c = 73.992 Å, respectively. Preliminary X-ray diffraction data analysis and molecular-replacement solution revealed the presence of one monomer per asymmetric unit

  7. Identification of a functional enhancer variant within the chronic pancreatitis-associated SPINK1 c.101A>G (p.Asn34Ser)-containing haplotype.

    Science.gov (United States)

    Boulling, Arnaud; Masson, Emmanuelle; Zou, Wen-Bin; Paliwal, Sumit; Wu, Hao; Issarapu, Prachand; Bhaskar, Seema; Génin, Emmanuelle; Cooper, David N; Li, Zhao-Shen; Chandak, Giriraj R; Liao, Zhuan; Chen, Jian-Min; Férec, Claude

    2017-08-01

    The haplotype harboring the SPINK1 c.101A>G (p.Asn34Ser) variant (also known as rs17107315:T>C) represents the most important heritable risk factor for idiopathic chronic pancreatitis identified to date. The causal variant contained within this risk haplotype has however remained stubbornly elusive. Herein, we set out to resolve this enigma by employing a hypothesis-driven approach. First, we searched for variants in strong linkage disequilibrium (LD) with rs17107315:T>C using HaploReg v4.1. Second, we identified two candidate SNPs by visual inspection of sequences spanning all 25 SNPs found to be in LD with rs17107315:T>C, guided by prior knowledge of pancreas-specific transcription factors and their cognate binding sites. Third, employing a novel cis-regulatory module (CRM)-guided approach to further filter the two candidate SNPs yielded a solitary candidate causal variant. Finally, combining data from phylogenetic conservation and chromatin accessibility, cotransfection transactivation experiments, and population genetic studies, we suggest that rs142703147:C>A, which disrupts a PTF1L-binding site within an evolutionarily conserved HNF1A-PTF1L CRM located ∼4 kb upstream of the SPINK1 promoter, contributes to the aforementioned chronic pancreatitis risk haplotype. Further studies are required not only to improve the characterization of this functional SNP but also to identify other functional components that might contribute to this high-risk haplotype. © 2017 Wiley Periodicals, Inc.

  8. Summary of the report A.S.N. n. 2006 ENSTR 019 - IGAS n. RM 2007-015P on the Epinal radiotherapy accident; Resume du rapport ASN n. 2006 ENSTR 019 - IGAS n. RM 2007-015P sur l'accident de radiotherapie d'Epinal

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    The Nuclear Safety Authority (A.S.N.) and the General Inspection of Social Affairs (I.G.A.S.) have been in charge by the Ministry of an inquiry on the accident that has lead to the overdose of 23 patients irradiated for a prostate cancer at the Hospital of Epinal. This event constitutes the most important accident implying the ionizing radiations occurring in France. In may 2004, the protocol of conformal radiotherapy applied to the prostate cancers has been modified, it uses dynamics corners in place of static corners. This change supposes to modify the parameters definition that gives the calculation of irradiation intensity, that will not not be made for some of patients. This mistake could be changed if the in vivo dosimetry that allows to check the real dose received by the patients had been kept. but this line of safety has been removed, the use of dynamic corners making it ineffective. The period of over irradiation has been from the 6. may 2004 to the 1. august 2005. During the summer 2005, the radiotherapist and radio physicist find the overdose error by examining the patients files again. On the 15. september 2005, the two doctors of the service warn the director of the C.H.J.M. (hospital center of Jean Monnet) of the accident that informs D.D.A.S.S. (departmental direction of sanitary and social affairs) and A.R.H. (regional agencies of hospitalization). But the national authorities (A.F.S.S.A.P.S., A.S.N., I.R.S.N.) have not been warned, and so nobody can recognize the unsuitable character of the decided measures. Only seven patients are informed of the overdose of radiations they received during the last trimester 2005. The assistance to the victims is not organised the first year. All the patients have received usual treatments when they should receive a treatment by graft of mesenchymatous stem cells. Between september 2005 and september 2006 four patients died and ten present serious complications. propositions have been made to help the victims and

  9. Asn 362 in gp120 contributes to enhanced fusogenicity by CCR5-restricted HIV-1 envelope glycoprotein variants from patients with AIDS

    Directory of Open Access Journals (Sweden)

    Wang Bin

    2007-12-01

    Full Text Available Abstract Background CCR5-restricted (R5 human immunodeficiency virus type 1 (HIV-1 variants cause CD4+ T-cell loss in the majority of individuals who progress to AIDS, but mechanisms underlying the pathogenicity of R5 strains are poorly understood. To better understand envelope glycoprotein (Env determinants contributing to pathogenicity of R5 viruses, we characterized 37 full-length R5 Envs from cross-sectional and longitudinal R5 viruses isolated from blood of patients with asymptomatic infection or AIDS, referred to as pre-AIDS (PA and AIDS (A R5 Envs, respectively. Results Compared to PA-R5 Envs, A-R5 Envs had enhanced fusogenicity in quantitative cell-cell fusion assays, and reduced sensitivity to inhibition by the fusion inhibitor T-20. Sequence analysis identified the presence of Asn 362 (N362, a potential N-linked glycosylation site immediately N-terminal to CD4-binding site (CD4bs residues in the C3 region of gp120, more frequently in A-R5 Envs than PA-R5 Envs. N362 was associated with enhanced fusogenicity, faster entry kinetics, and increased sensitivity of Env-pseudotyped reporter viruses to neutralization by the CD4bs-directed Env mAb IgG1b12. Mutagenesis studies showed N362 contributes to enhanced fusogenicity of most A-R5 Envs. Molecular models indicate N362 is located adjacent to the CD4 binding loop of gp120, and suggest N362 may enhance fusogenicity by promoting greater exposure of the CD4bs and/or stabilizing the CD4-bound Env structure. Conclusion Enhanced fusogenicity is a phenotype of the A-R5 Envs studied, which was associated with the presence of N362, enhanced HIV-1 entry kinetics and increased CD4bs exposure in gp120. N362 contributes to fusogenicity of R5 Envs in a strain dependent manner. Our studies suggest enhanced fusogenicity of A-R5 Envs may contribute to CD4+ T-cell loss in subjects who progress to AIDS whilst harbouring R5 HIV-1 variants. N362 may contribute to this effect in some individuals.

  10. Extensive spectroscopic calculations of the 21 Λ-S and 74 Ω states of the AsN molecule including the spin–orbit coupling effect

    International Nuclear Information System (INIS)

    Liu, Hui; Shi, Deheng; Sun, Jinfeng; Zhu, Zunlue

    2015-01-01

    The potential energy curves (PECs) of 74 Ω states generated from the 21 Λ-S states of AsN molecule are studied for the first time for internuclear separations from 0.1 to 1.0 nm. Of these 21 Λ-S states, the X 1 Σ + , a′ 3 Σ + , 1 5 Σ + , 1 3 Δ, 1 3 Σ − , a 3 Π, 1 5 Π, 2 5 Σ + , 3 5 Σ + , 2 3 Δ, 2 3 Π, 3 3 Π, 3 5 Π, and A 1 Π states are found to be bound, and the 2 3 Σ + , 3 3 Σ + , 1 5 Σ − , 1 5 Δ, 2 5 Δ, 2 5 Π, and 1 7 Σ + states are found to be repulsive ones. The 3 3 Π state possesses the double well. The 2 5 Σ + , 3 5 Σ + , 3 5 Π, and 3 3 Π states possess the shallow well. The a′ 3 Σ + , 1 3 Σ − , 2 3 Π, 1 3 Δ, 1 5 Π, 2 5 Π, 3 5 Π, and 1 7 Σ + states are found to be the inverted ones with the spin–orbit coupling effect taken into account. The PECs are calculated using the CASSCF method, which is followed by the internally contracted MRCI approach with Davidson correction. Core–valence correlation and scalar relativistic corrections are included. The vibrational properties are evaluated for the 2 5 Σ + , 3 5 Σ + , and 3 5 Π states and the second well of the 3 3 Π state. The spin–orbit coupling effect is accounted for by the state interaction method with the Breit–Pauli Hamiltonian. The PECs are extrapolated to the complete basis set limit. The spectroscopic parameters are evaluated, and compared with available measurements and other theoretical results. The Franck–Condon factors and radiative lifetimes of the transitions from the a′ 3 Σ + 1 , a 3 Π 1 , A 1 Π 1 , 1 3 Δ 1 and a 3 Π 0− states to the X 1 Σ + 0+ state are calculated for several low vibrational levels, and some necessary discussion is performed. Analyses show that the spectroscopic parameters reported in this paper can be expected to be reliably predicted ones. - Highlights: • Effect of core–valence correlation and scalar relativistic corrections is included. • PECs are extrapolated to the CBS limit for the 21 Λ-S states and

  11. Guatemala: Ozbrojený konflikt 1960-1996 a současná situace: se zvláštním zaměřením na Máje

    OpenAIRE

    Vilímková, Olga

    2009-01-01

    Tématem disertační práce je Guatemala: ozbrojený konflikt v letech 1960 - 1996 a současná situace se zvláštním zaměřením na Máje. Příčiny ozbrojeného konfliktu druhé ploviny XX. století (a mnoha současných problémů) v Guatemale se nacházejí v hluboké minulosti země. Už v době koloniální se začaly formovat ekonomicko - sociální vztahy mezi dvěma antagonistickými vrstvami společnosti: kolonizátory a původním obyvatelstvem, které bylo porobeno, vytlačeno na okraj společnosti a využíváno jako pra...

  12. Motywacja i wpływ uprawiania jogi na stan zdrowia – badanie własne = Motivation and influence practicing yoga on health - own study

    Directory of Open Access Journals (Sweden)

    Katarzyna Gwis

    2016-09-01

      Motywacja i wpływ uprawiania jogi na stan zdrowia – badanie własne Motivation and influence practicing yoga on health - own study   Katarzyna Gwis1, Dominik Olejniczak2, Joanna Skonieczna2   1 Absolwentka kierunku Zdrowie Publiczne Wydział Nauki o Zdrowiu Warszawski Uniwersytet Medyczny   2 Warszawski Uniwersytet Medyczny Zakład Zdrowia Publicznego ul. Banacha 1a, 02-097 Warszawa   Słowa kluczowe: joga, zdrowie, zachowania zdrowotne Key words: yoga, health, health behavior   Streszczenie Wprowadzenie i cel pracy: Joga to jedna z form aktywności ruchowej. Joga oddziałuje na ciało i na umysł, dzięki czemu w prosty sposób pomaga zachować zdrowie. Celem badania była ocena wpływu uczestnictwa w zajęciach jogi na zdrowie. Materiał i metoda: Grupę badaną stanowiło 50 osób uczęszczających na zajęcia jogi. Udział w ankiecie był dobrowolny i poufny. Zastosowano autorski kwestionariusz. Wyniki: W grupie badanych, aż 48 osób (96% stwierdziło, że ćwiczenia jogi pozytywnie wpływają na kształtowanie nawyków ruchowych. Ponad połowa badanych - 26 osób (52% stwierdziła, że uczęszczanie na zajęcia jogi sprawiło, że wzrosło ich zainteresowanie zdrowym odżywianiem. Ponad połowa badanych – 26 osób (52% jako główną korzyść płynącą z ćwiczeń jogi wskazała wzrost sprawności fizycznej. Ponad połowa osób ankietowanych – 28 osób (56% zaobserwowała, że od kiedy ćwiczy jogę stany chorobowe występują rzadziej.  Wnioski: Analizując wyniki przeprowadzonego badania własnego oraz dyskusję można stwierdzić, iż w związku z udokumentowanym pozytywnym wpływem jogi na stan zdrowia warto jest propagować tą formę rekreacji ruchowej we wszystkich grupach wiekowych, poczynając od dzieci, a  kończąc na osobach w  podeszłym wieku.       Abstract   Introduction: Yoga is a form of physical activity. Yoga affects the body and the mind, making it an easy way to help maintain good health. The aim of this

  13. A Mosaic Expression of a Hb J-Amiens (HBB: c.54G > T; p.Lys18Asn) and its Interference with Hb A1c Analysis.

    Science.gov (United States)

    Schiemsky, Toon; Van Hoovels, Lieve; Desmet, Koen J O; Phylipsen, Marion; Harteveld, Cornelis L; Kieffer, Davy M J

    2015-01-01

    We report the case of a 56-year-old Caucasian woman in whom hemoglobinopathy screening was triggered following an aberrant Hb A1c analysis. Preliminary diagnosis of the hemoglobin (Hb) variant was obtained through cation exchange high performance liquid chromatography (HPLC) and gel electrophoresis. DNA analysis confirmed the presence of Hb J-Amiens [β17(A14)Lys→Asn; HBB: c.[54G > C or 54G > T)]. However, an unbalanced ratio between wild type and mutant signal after direct sequencing and a lower than expected percentage of this Hb variant led to the suggestion of a mosaic expression. Furthermore, different methods [capillary zone electrophoresis (CZE), cation exchange HPLC and boronate affinity] were tested to study the possible interference of this variant with Hb A1c measurements. These investigations showed a clinically relevant difference between the methods tested. Hb A1c analysis may lead to the discovery of new Hb variants or mosaicism for previously described Hb variants. This may have genetic consequences for the offspring of carriers and brings about the question of partner testing.

  14. The old and the new in prekallikrein deficiency: historical context and a family from Argentina with PK deficiency due to a new mutation (Arg541Gln) in exon 14 associated with a common polymorphysm (Asn124Ser) in exon 5.

    Science.gov (United States)

    Girolami, Antonio; Vidal, Josè; Sabagh, Marcela; Salagh, Marcela; Gervan, Nora; Parody, Maria; Peroni, Edoardo; Sambado, Luisa; Guglielmone, Hugo

    2014-07-01

    Prekallikrein (PK) is one of the clotting factors involved in the contact phase of blood. PK has an important historical role as its deficiency state represents the second instance of a clotting defect without bleeding manifestations, the first one being factor XII deficiency. PK deficiency is a rare clotting disorder. Moreover, only 11 patients have been investigated so far by molecular biology techniques. In this article, we briefly review some of the history around PK and also present some recent data on a newly identified family from Argentina suffering from PK deficiency. Two patients are homozygous whereas other family members are heterozygous. PK activity and antigen are 1% of normal in the homozygotes and around 60 to 70% of normal in the heterozygotes. As expected, all patients are asymptomatic of bleeding or thrombosis presentations. However, the two homozygotes showed essential hypertension. The PK deficiency in this family is due to a new mutation (Arg541Gln) in exon 14. The defect segregates together with a known polymorphism, Asn124Ser, in exon 5. The significance of the presence of hypertension in the two homozygotes is discussed in view of the extra coagulation effects of PK on vasodilation, vessel permeability, and the control of blood pressure. Structure function analysis indicates that the substitution of Arg with Gln probably impedes the transmembrane diffusion of the molecule, which therefore cannot be secreted in the homozygotes. The presence of hypertension in patients with PK deficiency has been previously reported in some but not all patients. Future research activities will probably concentrate on the effect of PK and other contact phase factors on the vascular system. Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

  15. α-Thalassemia Associated with Hb Instability: A Tale of Two Features. The Case of Hb Rogliano or α1 Cod 108(G15)Thr→Asn and Hb Policoro or α2 Cod 124(H7)Ser→Pro.

    Science.gov (United States)

    Musollino, Gennaro; Cardiero, Giovanna; Flagiello, Angela; La Porta, Gaetana; Lagona, Laura; Prezioso, Romeo; Qualtieri, Gabriele; Gaudiano, Carlo; Medulla, Emilia; Merlino, Antonello; Pucci, Piero; Lacerra, Giuseppina

    2015-01-01

    We identified two new variants in the third exon of the α-globin gene in families from southern Italy: the Hb Rogliano, α1 cod108 ACC>AAC or α1[α108(G15)Thr→Asn] and the Hb Policoro, α2 cod124 TCC>CCC or α2[α124(H7)Ser→Pro]. The carriers showed mild α-thalassemia phenotype and abnormal hemoglobin stability features. These mutations occurred in the G and H helices of the α-globin both involved in the specific recognition of AHSP and β1 chain. Molecular characterization of mRNA, globin chain analyses and molecular modelling studies were carried out to highlight the mechanisms causing the α-thalassemia phenotype. The results demonstrated that the α-thalassemia defect associated with the two Hb variants originated by different defects. Hb Rogliano showed an intrinsic instability of the tetramer due to anomalous intra- and inter-chain interactions suggesting that the variant chain is normally synthesized and complexed with AHSP but rapidly degraded because it is unable to form the α1β1 dimers. On the contrary in the case of Hb Policoro two different molecular mechanisms were shown: the reduction of the variant mRNA level by an unclear mechanism and the protein instability due to impairment of AHSP interaction. These data highlighted that multiple approaches, including mRNA quantification, are needed to properly identify the mechanisms leading to the α-thalassemia defect. Elucidation of the specific mechanism leads to the definition of a given phenotype providing important guidance for the diagnosis of unstable variants. PMID:25730315

  16. Obstacles towards small business development in the Emfuleni district / Guillaume de Swardt

    OpenAIRE

    De Swardt, Guillaume

    2006-01-01

    Small businesses are essential for stimulating growth and alleviating poverty. The main aim of this study is to gather information regarding obstacles that withhold the small business sector from further development. This implies that attention will be given to the role of the entrepreneur, the small business sector and the role that business planning plays, in order to identify, minimise and possibly overcome the identified obstacles. Although small business is not an absolute...

  17. Pärt, Arvo. Fratres (version 1980). Cantus in memoriam Benjamin Britten / Guillaume Connesson

    Index Scriptorium Estoniae

    Connesson, Guillaume

    1995-01-01

    Uuest heliplaadist "Pärt, Arvo. Fratres (version 1980). Cantus in memoriam Benjamin Britten. Summa. Spiegel im Spiegel. Festina lente. Tabula rasa: Tasmin Little (violon), Martin Roscoe (piano), Bournemouth Sinfonietta, Richard Studt" EMI Classics 565 031-2 (CD: 98 F). 1993. TT: 1h 03'42"

  18. Guillaume Cuchet, director. Le Purgatoire. Fortune historique et historiographique d’un dogme

    Directory of Open Access Journals (Sweden)

    Renán Silva

    2013-07-01

    Full Text Available En Colombia no son tan frecuentes los balances historiográficos, o por lo menos no son tan frecuentes como se desearía —abundan más bien los “estados del arte” en las páginas iniciales de una tesis o de un trabajo de maestría, en general con buena información sobre un tema particular, tratado de manera monográfica—. Pero balances historiográficos, en sentido estricto, es decir trabajos que den cuenta de la totalidad de la investigación en un campo determinado —estudiando con detalle las formas de tratamiento del tema desde el punto de vista de los enfoques, los métodos y las técnicas—, que muestren al mismo tiempo “el ascenso y la caída” de un cierto tema de investigación y la forma de acercamiento dominante en un momento determinado, y que además pongan en relación ese tema particular con el conjunto de la producción historiográfica en un área mayor (aquí, por ejemplo, las historias de las mentalidades y de la cultura y con los avatares mismos de la sociedad de que se trate, ese tipo de balances historiográficos no son frecuentes entre nosotros, y hay que lamentarlo.  Le Purgatoire. Fortune historique et historiographique d’ un dogme cumple con creces esa necesidad que señalamos, y puede ser visto grosso modo como un modelo de trabajos que hay que iniciar o continuar, si existen, en muchos campos de la investigación histórica en el país.

  19. Order of the 24. of November 2009 approving the decision n. 2009-DC-0147 made on the 16. of July 2009 by the Nuclear Safety Authority (ASN) defining duties practice conditions of a person having abilities in radioprotection and external to the establishment to apply the R. 4456-4 article of the Labour Code

    International Nuclear Information System (INIS)

    2009-01-01

    This legal text approves a decision made by the ASN defining notably the content and formalization of the agreement between the employer and the person having abilities in radioprotection and who is external to the establishment. It also defines their reciprocal obligations. It indicates the groups of electrical equipment generating X rays and the groups of professional activities which are concerned by this order. It finally defines the requirements related the interventions of this person (frequency, mandatory interventions) with respect to the equipment groups

  20. Hearing of Mrs Marie-Pierre Comets and Mr Marc Sanson, commissioners of the Nuclear Safety Authority (ASN), as well as Mrs Marie-Claude Dupuis, general manager of the National Agency for the management of radioactive wastes (ANDRA), on the nuclear events in Cadarache

    International Nuclear Information System (INIS)

    2009-01-01

    Representatives of the French Nuclear Safety Authority (ASN, Autorite de Surete Nucleaire) and of the French National Agency for the Management of Radioactive Wastes (ANDRA, Agence nationale pour la gestion des dechets radioactifs) answer questions about an incident which occurred in a workshop, where MOx fuel was produced and which is now being dismantled. It appeared that the amount of plutonium present in the gloveboxes has been largely under-assessed: 8 kg were expected, 22 kg of plutonium have already been recovered, and finally 39 kg might be recovered by the end the dismantling operations. Other issues are addressed like the ANDRA's projects of radioactive material geological storage, their technical and institutional aspects, and then again about the late declaration of the Cadarache incident

  1. The dating of the fourth volume of Guillaume-Antoine Olivier's "Entomologie, ou histoire naturelle des insectes".

    Science.gov (United States)

    Bousquet, Yves

    2018-01-01

    Despite the title page is dated 1795, the fourth volume of Olivier's Entomologie, ou histoire naturelle des insectes was issued in two parts, one probably in 1795 and the second in 1800. All new taxa made available in this work have previously been dated 1795 in the literature. A list of new species described in 1795 and a list of those that have to be dated 1800 are appended. The genus Necrobia should be credited to Latreille, 1797, not Olivier, 1795.

  2. L’amour courtois et la symbolique de la Kénose dans le motet 10 de Guillaume de Machaut

    Directory of Open Access Journals (Sweden)

    Charlène Mercier

    2009-04-01

    Full Text Available Dans l’esprit médiéval, chaque chose est ordonnée par l’harmonie de Dieu, décidée selon un plan unique. Il en va ainsi du macrocosme, de l’ordre régissant l’univers tout entier, au microcosme, allant jusqu’à gouverner la structure interne de chaque création de l’intellect humain. D’après la première épître de saint Paul aux Corinthiens (13, 12 : « Videmus nunc per speculum in aenigmate, tunc autem facie ad faciem . » Étudier une pièce de l’époque reviendrait-il à décrypter les liens cachés e...

  3. "Better a Live Sparrow than a Stuffed Eagle": Towards a Translation of Guillaume de Machaut's "Dit de l'Alerion."

    Science.gov (United States)

    Thaon, Brenda

    1978-01-01

    A discussion of some special problems in translating medieval poetry into a modern language while conveying the original meaning, emotional tone and word play. The discussion centers on the poetry of Machaut, a medieval poet of interest to English scholars because of his influence on Chaucer. (AMH)

  4. Order of the 29. of January 2010 approving the decision n. 2009-DC-0146 made on the 16. of July 2010 by the Nuclear Safety Authority (ASN), modified by the decision n. 2009-DC-0162 of the 20. of October 2009, defining the list electric equipment generating X rays which are hold or used for purposes of biomedical research or medical, dental, forensics or veterinary diagnosis and submitted to a declaration regime on the account of the first paragraph of the article R. 1333-19 of the Public Health code and abrogating the 23 April 1969 order related to equipment and installations using ionizing radiations on medical purposes

    International Nuclear Information System (INIS)

    2010-01-01

    This legal document indicates the list of equipment which must now be declared to the ASN (the French Nuclear Safety Authority), specifies that any use of these equipment in conditions other than normal requires an authorization on the account of an article of the Public Health Code. A list of equipment is given which are used in medical and forensics radio-diagnosis, in biomedical research, in dental and veterinary radio-diagnosis

  5. Order of the 29. of January 2010 approving the decision n. 2009-DC-0146 made on the 16. of July 2010 by the Nuclear Safety Authority (ASN), modified by the decision n. 2009-DC-0162 of the 20. of October 2009, defining the list electric equipment generating X rays which are hold or used for purposes of biomedical research or medical, dental, forensics or veterinary diagnosis and submitted to a declaration regime on the account of the first paragraph of the article R. 1333-19 of the Public Health code and abrogating the 23 April 1969 order related to equipment and installations using ionizing radiations on medical purposes

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2010-07-01

    This legal document indicates the list of equipment which must now be declared to the ASN (the French Nuclear Safety Authority), specifies that any use of these equipment in conditions other than normal requires an authorization on the account of an article of the Public Health Code. A list of equipment is given which are used in medical and forensics radio-diagnosis, in biomedical research, in dental and veterinary radio-diagnosis

  6. Lessons learned from the ASN Renal Educator Listserv and survey.

    Science.gov (United States)

    Hoenig, Melanie P; Shapiro, Evelyn; Hladik, Gerald A

    2013-06-01

    Nephrology ranks next to last in career choices among US medical school graduates. The American Society of Nephrology established a Workforce Committee to help address this issue. Surveys of US medical students indicate that experiences during kidney pathophysiology courses in the preclerkship years may impact their decision to consider a career in nephrology. In October of 2011, preclinical kidney physiology and pathophysiology course directors at US medical schools were surveyed about teaching methods, curricular content, resources, and institutional support for teaching to identify what worked well and what impairs their teaching efforts. A Listserv of these educators, the American Society of Nephrology Renal Educators Listserv, was used to electronically administer the survey. Course leaders from 62 of 114 (54.4%) surveyed medical schools responded. Most of these educators are nephrologists, but physiologists and other clinicians also lead courses; 60% of course directors noted that lectures are videotaped, resulting in decreased attendance. A range of resources is used: 68% use audience response systems, 16% use the simulation center, and none of the educators indicated use of social media (such as Twitter or Facebook); 50% of respondents receive no remuneration, and 68% receive no full-time equivalent for their efforts. Audience response systems, virtual microscopy, and flash animations were identified as valuable teaching tools. Course directors, during subsequent dialogue on the American Society of Nephrology Renal Educators Listserv, have cited incorporation of case scenarios and integration of clinical exposure during preclinical years as methods that inspired interest. Hopefully, adoption of such approaches will ultimately serve to stimulate interest in nephrology.

  7. Současný pohled na koenzym Q

    Czech Academy of Sciences Publication Activity Database

    Rauchová, Hana; Vokurková, Martina

    2009-01-01

    Roč. 103, č. 1 (2009), s. 32-39 ISSN 0009-2770 R&D Projects: GA MŠk(CZ) 1M0510 Institutional research plan: CEZ:AV0Z50110509 Keywords : coenzyme Q * mitochondria * dietary supplement Subject RIV: CE - Biochemistry Impact factor: 0.717, year: 2009

  8. A petrographic study of Precambrian quartzites from Goa coast

    Digital Repository Service at National Institute of Oceanography (India)

    Wagle, B.G.; Rajamanickam, G.V.

    tourmaline, topaz, zircon, rutile, fluorite, apatite and hornblende lead to a source of acidic composition. The introduction of tourmaline and fluorite in the wacke, the grade of metamorphism and the source of the wacke have been discussed...

  9. The dating of the fourth volume of Guillaume-Antoine Olivier’s “Entomologie, ou histoire naturelle des insectes”

    Science.gov (United States)

    Bousquet, Yves

    2018-01-01

    Abstract Despite the title page is dated 1795, the fourth volume of Olivier’s Entomologie, ou histoire naturelle des insectes was issued in two parts, one probably in 1795 and the second in 1800. All new taxa made available in this work have previously been dated 1795 in the literature. A list of new species described in 1795 and a list of those that have to be dated 1800 are appended. The genus Necrobia should be credited to Latreille, 1797, not Olivier, 1795. PMID:29674858

  10. Ferdinand Buisson (1841-1932 educador e pacifista: as influências libertárias de James Guillaume e Paul Rodin

    Directory of Open Access Journals (Sweden)

    Martine Brunet

    2012-07-01

    Full Text Available  Ferdinand Buisson foi uma grande figura da Terceira República. Alto funcionário da instrução pública, professor de pedagogia na Sorbonne, deputado do partido radical e radical-socialista, recebeu o Prêmio Nobel da Paz em 1927. Suas atividades foram numerosas e imensas, tanto no âmbito religioso como político e pedagógico; ao longo de sua vida, esses três campos estiveram interpenetrados. Muito cedo ingressou na vida pública, e notabilizou-se pela tomada de posições radicais contra a guerra, participando dos primeiros congressos da Liga da Paz e da Liberdade, na Suíça. Desde a queda do Império, participou da vida política da municipalidade do 17° arrondissement de Paris, que tinha como adjunto do prefeito o anarquista Benoit Malon. Buisson entrou na Guarda Nacional, participou ativamente das iniciativas políticas e sociais da municipalidade. Foi co-fundador do jornal anarquista A República dos Trabalhadores, com Aristide Rey, os irmãos e Benoit Malon. Foi durante a guerra e o sítio em Paris que fundou um orfanato. Esse estabelecimento laico recebia subsídios da Internacional e ministrava uma educação integral. resultante das idéias anarquistas de Paul Robin. Após a derrota da Comnna, o orfanato foi transferido para Cempuis, na região do Oise. Em 1879, Ferdinand Buisson foi nomeado diretor do ensino primário, chamou a Paul Robin para dirigir o orfanato segundo os métodos da educação libertária. O estabelecimento e seu diretor são violentamente atacados pelos reacionários católicos. A experiência inovadora de Cempuis terminou em 1894.Palavras-chave: educação libertária, liberdade, pacifismo.   Résumé Ferdinand Buisson est une grande figure de la Troisieme République. Haut fonctionnaire de l'instruction publique, professeur de pédagogie à Ia Sorbonne, député du parti radical et radical-socialiste, il reçut en 1927 le prix Nobel de la Paix. Ses activités furent nombreuses et immenses, à Ia fois dans le domaine religieux, politique et pédagogique; ces trois domaines se sont interpénétrés tout au long de sa vie. Très tôt il entre dans la vie publique et il se fait remarquer par des prises de positions radicales contre la guerre en participant aux premiers congres de Ia Ligue de la Paix et de la Liberté en Suisse. Des Ia chute de l'Empire, il participe à Ia vie poli tique du 17° arrondissement de Paris, dont l' adjoint au maire est l'anarchiste Benoit Malon. Il s'engage dans Ia Garde Nationale, il participe activement aux initiatives politiques et sociales de Ia municipalité, il est cofondateur d'un journal anarchiste Lu République des Travailleurs avec Aristide Rey, les freres Reclus et Benoit Malon. C'est dans Ia tourmente de la guerre, d'un Siege de Paris qu'il crée un orphelinat. Cet établissement lai'que reçoit des subsides de l'Internationale et donne une éducation intégrale issue des idées de I'anarchiste Paul Robin. Apres Ia défaite de la Commune, l'orphelinat est transféré à Cempuis dans l’Oise. En 1879, Ferdinand Buisson nommé directeur de l'enseignement primaire fait appel à Paul Robin pour diriger l'orphelinat selon ses méthodes libertaires. L'établissement et son directeur sont pris violemment à partis par les catholiques réactionnaires. l'expérience de Cempuis est finie en 1894. Mots-clé: éducation libertaire, liberté, internationale. 

  11. Les yeux de la nation. Photographie amateur et société dans l’Allemagne de Guillaume II

    Directory of Open Access Journals (Sweden)

    Christian Joschke

    2009-12-01

    Full Text Available 1880-1910 : trois décennies au cours desquelles les images, et singulièrement les images photographiques, prirent une importance capitale dans la vie publique et qui virent naître dans la bourgeoisie libérale allemande un projet ambitieux : elle a en effet investi la société civile de la fonction de construire une culture visuelle éclairée sur les fondements d’une structure associative, de ses pratiques délibératives et de son idéal éducatif. Cette bourgeoisie encadra l’essor de la photograph...

  12. UNE MYTHOCRITIQUE DE LA CHANSON DE GUILLAUME EST-ELLE POSSIBLE ? DU VISIBLE À L’INVISIBLE DANS LA GESTE DE GUILLAUME D’ORANGE ET DE SES COMPAGNONS DE GUERRE (Is The Song of William susceptible to myth criticism? From the visible to the invisible in the Old French epic of William of Orange and his companions in arms

    Directory of Open Access Journals (Sweden)

    Ján Živčák

    2015-06-01

    Full Text Available The main aim of the present study is to examine the appropriateness of myth criticism methods in the context of medieval French literature. The first part of the essay is conceived as a brief confrontation, opposing the theory of Mircea Eliade concerning the repetition of the Hero Archetype in traditional epics to some textual elements in the chansons de geste, analogous to “visible mythemes” which tend to appear in popular epic poetry. Through a series of comparisons, we come to the conclusion that the interpretation to which the “eliadian” scheme leads us expands the boundaries of a literary myth (an academic invisible and rather aspires to a “theology of text” (a lived invisible. The second part of the article provides the reader with a practical application of the results of the preceding reflections, and includes an interpretation of the story of Vivien based on the Christian concept of Imitatio Christi.

  13. LA RÉÉCRITURE DU MYTHE D’ORPHÉE DANS LE BESTIAIRE OU CORTÈGE D’ORPHÉE DE GUILLAUME LA RÉÉCRITURE DU MYTHE D’ORPHÉE DANS LE BESTIAIRE OU CORTÈGE D’ORPHÉE DE GUILLAUME APOLLINAIRE

    Directory of Open Access Journals (Sweden)

    Julie Dekens

    2009-04-01

    Full Text Available Cette oeuvre se compose de trente courts poèmes – pour l’essentiel des quatrains formés d’octosyllabes ou d’alexandrins – accompagnés chacun d’une gravure sur bois de Raoul Dufy, auxquels sont associés des animaux dont le nom constitue le titre de ces pièces poétiques. Apollinaire joue donc dans ce cadre avec les motifs orphiques, aventure qui semble d’autant plus périlleuse si l’on s’aperçoit que ces thématiques ont été le terrain de jeu de nombreux poètes avant lui…Cette oeuvre se compose de trente courts poèmes – pour l’essentiel des quatrains formés d’octosyllabes ou d’alexandrins – accompagnés chacun d’une gravure sur bois de Raoul Dufy, auxquels sont associés des animaux dont le nom constitue le titre de ces pièces poétiques. Apollinaire joue donc dans ce cadre avec les motifs orphiques, aventure qui semble d’autant plus périlleuse si l’on s’aperçoit que ces thématiques ont été le terrain de jeu de nombreux poètes avant lui…

  14. "Občasné" a "dočasné" rezidence Jana Lucemburského v Lucembursku, Francii a Itálii

    Czech Academy of Sciences Publication Activity Database

    Benešovská, Klára

    2008-01-01

    Roč. 11, - (2008), s. 147-167 ISSN 0862-979X. [Dvory a rezidence ve středověku II. Praha, 18.10.2007-19.10.2007] Institutional research plan: CEZ:AV0Z80330511 Keywords : Gothic architecture * 14th century * John of Luxembourg * residences Subject RIV: AL - Art, Architecture, Cultural Heritage

  15. ASN guide project: complete cleaning-up modalities acceptable in nuclear base installations in France - ASN guide project nr 14 - Release of 21/06/2010

    International Nuclear Information System (INIS)

    2010-01-01

    As any nuclear base installation evolves during its exploitation, some premises or buildings may change of use or be destroyed. In the first case, a complete cleaning up might be necessary to get rid of radioactive products. In order to deal with this issue, after a brief recall of the regulatory context and references, and a recall of the general doctrine of waste management in nuclear base installations in France, this guide project presents the principles for a complete cleaning up of structures belonging to an area containing nuclear wastes. It describes the modalities of definition of three different and successive defence lines. It defines the requirements in performing cleaning-up operations, the requirements in terms of quality insurance, and the different administrative procedures (before, during and after cleaning-up works). Some particular cases are addressed

  16. Josquin og stemmernes klang. Et forslag om analyse af vokal instrumentation

    DEFF Research Database (Denmark)

    Christoffersen, Peter Woetmann

    2002-01-01

    Vokalmusik, instrumentation, klang, klangmusik, Josquin Desprez, Antoine Brumel, Johannes Ockeghem, Guillaume Du Fay, messe, contrapunctus......Vokalmusik, instrumentation, klang, klangmusik, Josquin Desprez, Antoine Brumel, Johannes Ockeghem, Guillaume Du Fay, messe, contrapunctus...

  17. Impressions from a visit by the ASN of the Laue Langevin Institute research reactor in Grenoble

    International Nuclear Information System (INIS)

    Nifenecker, H.

    2011-01-01

    After having recalled some specific characteristics of the Laue Langevin Institute research reactor (fuel type, cooling system, power, fuel management, fuel storage pool), the author reports the examination of the emergency procedures and of the reactor maintenance. He describes two exercises which respectively simulated the occurrence of an earthquake and that of a flooding due to a dam breaching

  18. Regionalismus v současné české architektuře

    Czech Academy of Sciences Publication Activity Database

    Švácha, Rostislav

    2006-01-01

    Roč. 6, č. 3 (2006), s. 48-51 ISSN 1801-089X Institutional research plan: CEZ:AV0Z80330511 Keywords : Czech architecture * regionalism * 20th century Subject RIV: AL - Art, Architecture, Cultural Heritage

  19. Cloud computing: vývoj a současný stav

    Directory of Open Access Journals (Sweden)

    Václav Sova Martinovský

    2018-06-01

    Full Text Available This article introduces the area of cloud computing with emphasis on businesses and organizations. It includes research of published papers on topic of cloud computing based on metaanalysis and SLR. Summary of relevant advantages both from the user and provider perspective was assembled as well as a list of barriers to even broader adoption of the cloud computing technology. Three databases (EBSCO, ProQuest and Web of Science were used as a source of data for the literature review phase. An analysis of the number of articles dealing with the cloud computing top has been carried out to date. The annual number of publications is compared to the size of the cloud computing market. Frequency analysis at a keyword level was performed in the 25,000-records dataset gathered from the WoS database. This analysis was conducted over the entire monitored period, and only for 2017, to capture the latest trends. There were identified the most frequently used key words in publications about cloud computing: virtualization, security, big data, mobile cloud computing and internet of things. For each of these keywords, a brief summary is given based on the literature search, with an emphasis on the outlook for the future and market position.

  20. Status of radiation protection in interventional radiology. Assessment of inspections in 2009 by the ASN

    International Nuclear Information System (INIS)

    2011-01-01

    This report first describes the organization of inspections performed in health institutions, indicates the inspected establishments, the types of fixed installations in interventional radiology, the use of imagery in the operating theatre, and discusses the regulatory arrangements applicable to interventional radiology (in the Public Health Code, in the Labour Code). Then, the report discusses the results of inspections regarding radiation protection in interventional radiology: application of public health code arrangements (justification, patient training in radiation protection, radiological procedures and protocols, patient dosimetry monitoring), application of Labour Code arrangements (designation of the person with expertise in radiation protection, risk assessment and delimitation of monitored and controlled areas, workstation analysis, workers' training in radiation protection, individual protection equipment, workers' dosimetric monitoring, workers' medical monitoring, radiation protection technical controls), significant events, radiation protection in operating theatre. Propositions are stated regarding the differences noticed within or between the health establishments, the methodological and organisational difficulties faced by persons with expertise in radiation protection (PCR), the need of an interdisciplinary team

  1. Conserved water-mediated H-bonding dynamics of catalytic Asn ...

    Indian Academy of Sciences (India)

    Prakash

    Extensive energy minimization and molecular dynamics simulation studies up to 2 ns ... Conserved water in molecular recognition; MD simulation; plant cysteine protease ..... Mustata G and Briggs J M 2004 Cluster analysis of water molecules.

  2. Tazatel, dotazovaný - dočasní "přátelé"

    Czech Academy of Sciences Publication Activity Database

    Nosková, Jana

    -, č. 35 (2004), s. 121-126 ISSN 1211-5770 Institutional research plan: CEZ:AV0Z9058907 Keywords : ethic s * fieldwork * relationship between interviewew and interviewee Subject RIV: AC - Archeology, Anthropology, Ethnology

  3. Vztah theologie a filosofie u Órigena a úkoly současné dogmatiky

    Czech Academy of Sciences Publication Activity Database

    Milko, Pavel

    2007-01-01

    Roč. 2, č. 2 (2007), s. 3-29 ISSN 1337-0111 R&D Projects: GA ČR GP401/07/P534 Institutional research plan: CEZ:AV0Z90920516 Keywords : Órigenés, ca 185-ca 253 * doctrinal the ology * philosophy Subject RIV: AA - Philosophy ; Religion

  4. Současné poznatky o obecné strupovitosti hlíz brambor

    Czech Academy of Sciences Publication Activity Database

    Krištůfek, Václav; Diviš, J.; Marečková, M.; Kopecký, J.

    2015-01-01

    Roč. 63, č. 2 (2015), s. 75-76 ISSN 0139-6013 R&D Projects: GA MZe QJ1210359 Institutional support: RVO:60077344 Keywords : common scab * potatoes Subject RIV: GF - Plant Pathology, Vermin, Weed, Plant Protection

  5. Estetika ve sportu v současné době

    OpenAIRE

    Fiřt, Martin

    2009-01-01

    Thesis title: The present sport aesthetics Aim of the thesis: To define an aestethics of sports in 21st century, it's particular appearance, essence and development.Description of terms "aesthetics", "sport", "experienced aesthetics of sport" , "the beauty of the human body". As well as terms "harmony in sports", Greek kalokaghatia and modern trends in sport disciplines. Method: A background research was conducted based on available specialised literature. Compilation of knowledge was created...

  6. Thirteen years and counting: Outcomes of a concurrent ASN/BSN enrollment program.

    Science.gov (United States)

    Heglund, Stephen; Simmons, Jessica; Wink, Diane; D'Meza Leuner, Jean

    In their 2011 report, The Future of Nursing, the Institute of Medicine called for 80% of the nursing workforce to be comprised of baccalaureate prepared Registered Nurses by the year 2020. One suggested approach to achieve this goal is the creation of programs that allow students to progress through associate and baccalaureate nursing preparation simultaneously. This paper describes the University of Central Florida's 13-year experience after implementing a Concurrent Enrollment Program. Development and structure of the program, advisement and curriculum details, facilitators and barriers are described. Data on National Council Licensure Examination for Registered Nurses pass rates, completion rates, comparison with traditional RN-BSN students, and progression to graduate school are also included. The Concurrent Program model described here between a specific university and state college partners, demonstrated positive outcomes that support achievement of the Institute of Medicine's goals. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. Assessment of pressurized nuclear equipment compliance - ASN guide no. 8, version of 04/09/2012

    International Nuclear Information System (INIS)

    2012-01-01

    This document describes the modalities according to which the compliance assessment of pressurized nuclear equipment must be performed by inspecting organisations according to a decree of December 1999 and an order of December 2005. After an indication of reference documents and some technical definitions, this guide describes the equipment classification and the principles of intervention. These principles concern the assessment request and the various assessment tasks to be performed by the inspecting organisation

  8. Květena Brna - současný stav poznání

    Czech Academy of Sciences Publication Activity Database

    Lososová, Z.; Šumberová, Kateřina; Tichý, L.; Danihelka, Jiří; Vymazalová, M.

    2015-01-01

    Roč. 63, č. 6 (2015), s. 289-292 ISSN 0044-4812 R&D Projects: GA ČR GB14-36079G Institutional support: RVO:67985939 Keywords : grid mapping * neophytes * city landscape Subject RIV: EH - Ecology, Behaviour

  9. Kluzáky v současném letectví

    OpenAIRE

    Schoř, Pavel

    2009-01-01

    Tato bakalářská práce pojednává v úvodu o stručné historii kluzáku. Druhá kapitola je věnována vysvětlení základních principů letu bezmotorového letadla včetně způsobu, jak může bezmotorový kluzák stoupat. Jsou zde také uvedeny používané způsoby vzletu kluzáků. Ve všech kapitolách jsou příklady uváděné především na kluzáku Standard Cirrus, je to zejména kvůli jeho velkému rozšíření v ČR a jeho snadné finanční dostupnosti. Ve třetí kapitole jsou rozebrány jednotlivé přírodní principy, které js...

  10. Gastroturismus - aktuální trend v současném turismu

    OpenAIRE

    Šejdová, Barbora

    2013-01-01

    This bachelor thesis deal with culinary tourism, as a new trend of tourism. Based on the definition of the field uses the scientific method of bibliographic search and statistical data. It focuses on the concept of inclusion in the typology of tourism. It issues the impact of tourism on the economy and the role of multiplier effects within the general theme. The practical part deals with culinary tourism and it's conception in Austria. First general issues of Austrian tourism are analyzed, wi...

  11. Heterologous gln/asn-rich proteins impede the propagation of yeast prions by altering chaperone availability.

    Directory of Open Access Journals (Sweden)

    Zi Yang

    Full Text Available Prions are self-propagating conformations of proteins that can cause heritable phenotypic traits. Most yeast prions contain glutamine (Q/asparagine (N-rich domains that facilitate the accumulation of the protein into amyloid-like aggregates. Efficient transmission of these infectious aggregates to daughter cells requires that chaperones, including Hsp104 and Sis1, continually sever the aggregates into smaller "seeds." We previously identified 11 proteins with Q/N-rich domains that, when overproduced, facilitate the de novo aggregation of the Sup35 protein into the [PSI(+] prion state. Here, we show that overexpression of many of the same 11 Q/N-rich proteins can also destabilize pre-existing [PSI(+] or [URE3] prions. We explore in detail the events leading to the loss (curing of [PSI(+] by the overexpression of one of these proteins, the Q/N-rich domain of Pin4, which causes Sup35 aggregates to increase in size and decrease in transmissibility to daughter cells. We show that the Pin4 Q/N-rich domain sequesters Hsp104 and Sis1 chaperones away from the diffuse cytoplasmic pool. Thus, a mechanism by which heterologous Q/N-rich proteins impair prion propagation appears to be the loss of cytoplasmic Hsp104 and Sis1 available to sever [PSI(+].

  12. Media w życiu małego dziecka. Badania własne

    OpenAIRE

    Kołodziejczyk, Anna

    2012-01-01

    Over the past decade infants' and toddlers' time spent with media has notably increased. At present, there is limited empirical knowledge regarding how children in different countries use media, how environmental variables influence children's activities. Presented study examined 343 children's aged 0;6 to 6;6 media use. The study provides baseline data about: which media do very young children use, and how much time do they spend using them, how old are children when they first starts using ...

  13. Současné politické konflikty v oblasti Afrického rohu

    Directory of Open Access Journals (Sweden)

    Alemayehu Kumsa

    2012-12-01

    Full Text Available Violent conflict is very old in human society. The development of military technology brought with itself the worst tragedies loss of human live and material devastation in the second half of 20th century in the Horn of Africa. This region is one of the centers of various political violent conflicts in the world, according to length of these violent conflicts, the number of death of people, mainly civilian, refugees and internal displaced persons (IDP. This study elucidates the root causes of long wars in the Horn of Africa focusing mainly on South Sudan and Somalia. It also illustrates how the Super Powers during the Cold War helped their client states to prolong the suffering of people in the region. When Socialist system disappeared from Eastern Europe, Mengistu Haile Mariam's and Siyad Barre's regime ignominiously collapsed. In Ethiopia Amhara power elite, who ruled the Empire state from 1889 to 1991 lost their state power and Tigrian guerrilla fighters captured it through the power of the gun, Eritrea gained its independence from Ethiopia, South Sudan is emerging from long heinous war to independence. The violent conflict in Somalia transformed after the old regime demise in 1991 and the new leaders unable to build new central government. Somalia is fragmented and became the good example of failed state in the theory of contemporary political sociology. The paper tries to explain these complex violent conflicts in this part of Africa.

  14. Současné politické konflikty v oblasti Afrického rohu

    Directory of Open Access Journals (Sweden)

    Alemayehu Kumsa

    2018-01-01

    Full Text Available Violent conflict is very old in human society. The development of military technology brought with itself the worst tragedies loss of human live and material devastation in the second half of 20th century in the Horn of Africa. This region is one of the centers of various political violent conflicts in the world, according to length of these violent conflicts, the number of death of people, mainly civilian, refugees and internal displaced persons (IDP. This study elucidates the root causes of long wars in the Horn of Africa focusing mainly on South Sudan and Somalia. It also illustrates how the Super Powers during the Cold War helped their client states to prolong the suffering of people in the region. When Socialist system disappeared from Eastern Europe, Mengistu Haile Mariam’s and Siyad Barre’s regime ignominiously collapsed. In Ethiopia Amhara power elite, who ruled the Empire state from 1889 to 1991 lost their state power and Tigrian guerrilla fighters captured it through the power of the gun, Eritrea gained its independence from Ethiopia, South Sudan is emerging from long heinous war to independence. The violent conflict in Somalia transformed after the old regime demise in 1991 and the new leaders unable to build new central government. Somalia is fragmented and became the good example of failed state in the theory of contemporary political sociology. The paper tries to explain these complex violent conflicts in this part of Africa.

  15. Současná věda o člověku

    Czech Academy of Sciences Publication Activity Database

    Černý, Viktor; Brůžek, J.

    2016-01-01

    Roč. 64, č. 5 (2016), s. 202 ISSN 0044-4812 Institutional support: RVO:67985912 Keywords : anthropology Subject RIV: AC - Archeology, Anthropology , Ethnology http://ziva.avcr.cz/files/ziva/pdf/soucasna-veda-o-cloveku.pdf

  16. ASN guide project. Protection of base nuclear installations against external flooding

    International Nuclear Information System (INIS)

    2010-01-01

    This guide aims at defining criteria to be taken into account to assess risks of flooding of a nuclear installation in case of external flooding, at proposing an acceptable method to assess such risks, at listing recommendations to define protection means adapted to the peculiarities of the flood risk and implemented by the operators with respect to the life phases of the installation, and in taking the climate change into account. The first part proposes an approach allowing the identification of reference situations which are to be taken into account for the flood risk. The second part deals with the quantification of parameters which characterize physical phenomena associated with these situations. The third part identifies the peculiarities of the flood risk as well as the guiding principles for designing options and protection mean selection with respect to a given flood risk

  17. SBMAC: Smart Blocking MAC Mechanism for Variable UW-ASN (Underwater Acoustic Sensor Network Environment

    Directory of Open Access Journals (Sweden)

    Soo-Hyun Park

    2010-01-01

    Full Text Available In this paper, several MAC scheduling methods applicable to an underwater environment are proposed. Besides, a new marine communication system model was proposed to improve the reliability of the proposed SBMAC method. The scheme minimizes transmission of control frames except for data transmission and various transmission methods and ACK methods can be used together. Simulation models are set indices and analysis of the underwater environment is established to conduct reliable simulations. Consequently, the performance improvement of the proposed method is verified with respect to delay time, data transmission rate, memory utilization, energy efficiency, etc.

  18. Generativita v současném empirickém výzkumu

    Czech Academy of Sciences Publication Activity Database

    Millová, Katarína; Blatný, Marek

    2016-01-01

    Roč. 60, č. 6 (2016), s. 609-621 ISSN 0009-062X R&D Projects: GA ČR GA15-22474S Institutional support: RVO:68081740 Keywords : generativity * personality * well-being * parenting * family * work Subject RIV: AN - Psychology Impact factor: 0.242, year: 2016

  19. Současné české neologismy z oblasti sportu

    OpenAIRE

    Filiačová, Sylva

    2014-01-01

    This bachelor thesis discusses the new appearance of substantive, adjective, verbal, and adverbial phrases in the sports area of the Czech language. The thesis is materially based on the database NEOMAT (Institute of the Czech Language of the Academy of Sciences of the Czech Republic), the databases of the Czech National Corpus, and the Newton Media archive. The first part of the thesis covers the definition of the concept of neologism. The following chapter discusses the ways of genesis of n...

  20. Progress in the domain of emissions tracking and environment radioactivity monitoring - Proceedings of the technical days organised by the SFRP Environment Section

    International Nuclear Information System (INIS)

    Calmet, Dominique; Calvez, Marianne; Rivasseau, Corinne Cea; Monfort, Marguerite; Manificat, Guillaume; Pierrard, Olivier; Couvez, Celine; Masson, Olivier; Bruno, Valerie; Renaud, Philippe; Genova, Zhana; Reynal, Nathalie; Le Coz, Eric; Tchilian, Nathalie; Diana, Jean-Jacques; Beguinel, Philippe; Cortes, Pierre; Puydarrieux, Stephane; Brun, Thierry; Devin, Patrick; Clavel, Benoit; Hemidy, Pierre-Yves; Gontier, Gilles; Delloye, Thierry; Mailliat, Alain; Ferreri, Giovanni; LECLERC, Elisabeth

    2015-11-01

    The Environment Section of the French Society of Radiation Protection (SFRP) organized a technical meeting on the progress made in the domain of emissions tracking and environment radioactivity monitoring. This document brings together the abstracts and the presentations (slides) of the different talks given at the meeting: 1 - Environment monitoring at the global, national and local scale: historical overview (Dominique CALMET, CEA); 2 - Evolution of radioactivity monitoring in the environment from 1960 to the present day (Guillaume MANIFICAT, IRSN); 3 - Euratom's legal framework (Zhana GENOVA, CTE); 4 - Main regulatory changes during the last decade (Nathalie REYNAL, ASN); 5 - Progress of standardization works on radioactive effluent emissions control and environment monitoring (Philippe BEGUINEL, BNEN); 6 - From operators' self-monitoring to ASN's inspections: a many components control system (Eric LE COZ, ASN); 7 - Control of effluents and emissions management at CEA Centres (Marianne CALVEZ, CEA); 8 - Liquid and gaseous effluents of ITER experimental facility: description and impacts (Pierre CORTES, IO); 9 - Effluents and emissions management strategy at AREVA NC La Hague facility (Stephane PUYDARRIEUX, AREVA); 10 - Radioactive effluents from nuclear facilities ongoing deconstruction: from dimensioning to real effluents (Benoit CLAVEL, EDF); 11 - Radionuclides decontamination process for liquid effluents using micro-algae at the laboratory scale (Corinne RIVASSEAU, CEA); 12 - Radioactive effluents from nuclear medicine services: management, monitoring and impact measurement methods (Nathalie TCHILIAN, ASN); 13 - Evolution history of effluents management and environment monitoring at the Solvay La Rochelle site (Thierry DELLOYE, SOLVAY); 14 - Different international approaches in effluents management and monitoring: example of French and German gaseous effluents - regulation, analyses, accounting rules (Jean-Jacques DIANA, ASN); 15 - Environment

  1. Bohuslav Havránek očima svých současníků

    Czech Academy of Sciences Publication Activity Database

    Dvořáčková, Věra

    2017-01-01

    Roč. 9, č. 1 (2017), s. 22-38 ISSN 1804-1221 R&D Projects: GA ČR(CZ) GA17-22085S Institutional support: RVO:67985921 Keywords : structuralism * Bohuslav Havránek Subject RIV: AB - History OBOR OECD: History (history of science and technology to be 6.3, history of specific sciences to be under the respective headings)

  2. Antonín Randa očima svých současníků

    Czech Academy of Sciences Publication Activity Database

    Velek, Luboš

    2016-01-01

    Roč. 155, č. 8 (2016), s. 673-688 ISSN 0231-6625 Institutional support: RVO:67985921 Keywords : Randa, Antonín * Faculty of Law, Czech University in Prague * Habsburg Monarchy, 1848-1918 Subject RIV: AB - History

  3. Soubor kostí z časně eneolitického objektu v Suchomastech, okr. Beroun

    Czech Academy of Sciences Publication Activity Database

    Kyselý, René

    2015-01-01

    Roč. 19, č. 2 (2015), s. 505-506 ISSN 1214-3553 Institutional support: RVO:67985912 Keywords : archaeozoology * Copper Age * cattle (Bos) * Jordanów culture Subject RIV: AC - Archeology, Anthropology, Ethnology

  4. Students’ health behaviors – own research = Zachowania zdrowotne studentów – badania własne

    Directory of Open Access Journals (Sweden)

    Magdalena Weber-Rajek

    2015-09-01

    Wyniki Średni wynik kwestionariusza IZZ dla całej badanej grupy wynosi 6,96 sten, co stanowi granicę między wynikami średnimi i wysokimi. Najlepsze wyniki kwestionariusza IZZ uzyskali studenci Dietetyki, najgorsze studenci Logistyki. W całej badanej grupie najlepsze wyniki uzyskano  kategorii „prawidłowe nawyki żywieniowe”, najgorsze w kategorii „praktyki zdrowotne”. Wnioski Istnieje potrzeba dalszych badań zachowań zdrowotnych młodzieży i upowszechniania programów promocji zdrowia na wszystkich etapach edukacji.   Słowa kluczowe: zachowania zdrowotne, studenci.

  5. Město a současná religiozita v pohledu sociologie náboženství

    Czech Academy of Sciences Publication Activity Database

    Nešpor, Zdeněk

    2016-01-01

    Roč. 11, č. 3 (2016), s. 78-91 ISSN 1211-7668 R&D Projects: GA ČR(CZ) GA14-15008S Institutional support: RVO:68378025 Keywords : Sociology of religion * urban sociology * city and religion Subject RIV: AA - Philosophy ; Religion

  6. The structure and energetic of AlAsn (n = 1-15) clusters: A first-principles study

    International Nuclear Information System (INIS)

    Guo Ling

    2010-01-01

    Geometric structures of AlAs n (n = 1-15) clusters are reported. The binding energy, dissociation energy, stability of these clusters are studied with the three-parameter hybrid generalized gradient approximation (GGA) due to Becke-Lee-Yang-Parr (B3LYP). Ionization potentials, electron affinities, hardness, and static polarizabilities are calculated for the ground-state structures within the same method. The growth pattern for AlAs n (n = 6-15) clusters is Al-substituted pure As n+1 clusters and it keeps the similar frameworks of the most stable As n+1 clusters (for example AlAs 6 , AlAs 7 , AlAs 9 , AlAs 14 and AlAs 15 clusters) or capping the different sides of the low-lying geometry of As n clusters (for example AlAs 8 , AlAs 10 , AlAs 11 , and AlAs 12 clusters). The Al atom prefer to occupy a peripheral position for n n (n = 1-5, 13) clusters. The odd-even oscillations from AlAs n (n = 5-15) in the dissociation energy, the second-order energy differences, the HOMO-LUMO gaps, the electron affinity, and the hardness are more pronounced. The stability analysis based on the energies clearly shows the AlAs n clusters from n = 5 with an even number of valence electrons are more stable than clusters with odd number of valence electrons.

  7. Increase of doses delivered to patients during medical imagery examinations. Conclusions of 16 September 2010 seminar organized by the ASN

    International Nuclear Information System (INIS)

    2011-01-01

    This document reports the contributions of the participants to the seminar which aimed at discussing the increase of doses delivered in medical imagery, and the actions to undertake to limit this increase. The authors recall the regulatory and legal context regarding the optimization of medical procedures and the organisation of radiation protection for medical exposures, comment the assessment of exposures by medical imagery in 2007, comment the CE marking of medical devices, the recent evolutions in dose optimization of radiology and scanography devices. Other sets of interventions address the application of the justification and optimization principle by professionals (in new scanography practices, in radio-paediatrics), the professional training issue (radiologist continuous training, electro-radiology operator training), the comparison between scanner and RMI (new fields for RMI, imagery benchmark), the assessment of professional practices (tools for continuous professional development of health professionals, medical imagery initiatives in Belgium), and international recommendations (by the IAEA, the WHO, the European Union)

  8. Cestovní ruch v Peru: vývoj, současný stav, vize

    OpenAIRE

    Pošepná, Zdeňka

    2009-01-01

    This thesis deals with development of tourism in Peru from the 19. century to the present-day by means of travel accounts written by travellers mainly from Europe and United States of America. Furthermore, the thesis analyzes present development of tourism in Peru. At first it evaluates today's Peruvian offer of tourist attractions presented by Peruvian guidebooks which compares with demand for these attractions trough the medium of contemporary foreign guidebooks. This analyze is completed b...

  9. Novo-viktoriánský román: současný trend britské fikce

    OpenAIRE

    Szilágyiová, Ester

    2010-01-01

    In 1966 when Jean Rhys wrote Wide Saragasso Sea no one would have guessed that she was starting a new literary movement whose very essence dwelled in re-thinking and rewriting Victorian myths and stories which Sally Shuttleworth named the retro-Victorian novel1. As a matter of fact, John Fowles's The French Lieutenant's Woman published in 1969, brought to public attention the parody of Victorian social, sexual and literary conventions2 but it was really in the 1980's and 1990's that many Brit...

  10. Vliv současných IT trendů na budoucnost šedé literatury

    OpenAIRE

    Savić, Dobrica

    2016-01-01

    This paper deals with emerging information technology (IT) and other trends and their impact on the grey literature. It is based on analysis of the most prevalent trends in general information management and new IT solutions, which will define and impact the digital future of related information management activities, as well as the grey literature. The analysis was done based on seven reports issued in 2016 by five world leading consulting and service companies that have a special interest i...

  11. Současná pozice cestovního ruchu ve světové ekonomice

    OpenAIRE

    Kadlecová, Anna

    2017-01-01

    The diploma thesis deals with the current position of tourism in the global economy. The work focuses on the regional layout of international tourism in terms of international arrivals, international tourism receipts and international tourism expenses. An emphasis is also put on the economic importance of tourism, mainly in relation to GDP, employment, price stability and balance of payment. The thesis is also focused on current issues in the field of international tourism - shared economy, C...

  12. Akademický slovník současné češtiny

    Czech Academy of Sciences Publication Activity Database

    Kochová, Pavla; Opavská, Zdeňka

    2016-01-01

    Roč. 99, č. 2 (2016), s. 57-83 ISSN 0027-8203 R&D Projects: GA MK DF13P01OVV011 Keywords : dictionary -making principles * lexicography * dictionary macrostructure * dictionary microstructure * monolingual dictionary Subject RIV: AI - Linguistics

  13. Liberalismus a jeho odůvodnění v současné politické filosofii

    OpenAIRE

    Cíbik, Matej

    2015-01-01

    This thesis attempts to answer one basic question: what we can philosophically say to justify liberalism as a mode of political existence of society. It is divided into three parts. In the first one, I critically survey two popular answers to this question, employing the concepts of self- ownership and value pluralism respectively. I argue that both of them are inadequate and unsatisfactory, mostly because they operate with a conception of person that is too thin for the justificatory task. I...

  14. Problematika současné role otce v rodině aneb krize identity otcovství

    OpenAIRE

    ŠVEHLOVÁ, Zdeňka

    2009-01-01

    The work deals with position of father in the modern-day family. The dominant position of father in family was lost within the empancipation of women inside the society. There become the system of patriarchy. The modern father has lost his position as a breadwinner of family, becouse of women try to assert at labour market too. The modern father has to find the new identity. He falls into split between traditional and modern position of father. In the work there is described the position of f...

  15. Safety and quality management for radiotherapy treatments - ASN guide nr 5 - Index 1 - Release of the 10/04/2009

    International Nuclear Information System (INIS)

    2009-01-01

    This guide aims at proposing a framework for the safety and quality management for radiotherapy treatments. It addresses the general requirements for the quality management system (general requirements and requirements related to documentation), the management responsibility, the resource management (human and hardware resources), the preparation and performance of activities allowing the taking into care of a patient from his first consultation to the post-treatment follow-up, the assessment, analysis and improvement of the quality management system

  16. Activation of an Asp-124→Asn mutant of haloalkane dehalogenase by hydrolytic deamidation of asparagine

    NARCIS (Netherlands)

    Pries, Frens; Kingma, Jacob; JANSSEN, Dick B

    1995-01-01

    Haloalkane dehalogenase hydrolyses various 1-halon-alkanes to the corresponding alcohols by covalent catalysis with formation of an alkyl-enzyme intermediate. The carboxylate function of the nucleophilic aspartate (Asp-124) that displaces the halogen during formation of the intermediate was changed

  17. Dopady dočasných změn valorizace důchodů

    Czech Academy of Sciences Publication Activity Database

    Janský, Petr

    -, č. 3 (2014), s. 67-78 ISSN 1212-415X Institutional support: RVO:67985998 Keywords : pension indexation * impact evaluation * Czech Republic Subject RIV: AH - Economics http://www.slu.cz/opf/cz/informace/acta-academica-karviniensia/casopisy-aak/aak-rocnik-2014/docs-3-2014/Jansky.pdf

  18. Problém Barák: Neruda z pohledu současné stylometrie

    Czech Academy of Sciences Publication Activity Database

    Plecháč, Petr; Flaišman, Jiří

    2017-01-01

    Roč. 65, č. 5 (2017), s. 743-769 ISSN 0009-0468 R&D Projects: GA ČR(CZ) GA14-31160S Institutional support: RVO:68378068 Keywords : stylometry * authorship attribution * textual criticism * Barák, Josef * Neruda, Jan Subject RIV: AJ - Letters, Mass-media, Audiovision OBOR OECD: Specific literatures

  19. Specifika současné ruské diskuse o Sovětském svazu

    Directory of Open Access Journals (Sweden)

    Zdeněk Bělonožník

    2014-06-01

    Full Text Available The aim of the article is to introduce contemporary Russian discussion on the USSR and Russia in broader political and publicist context. Both positive and negative interpretations of the Russian/Soviet history will be presented. The text does not pretend to provide exhaustive list of the particular “schools” and “movements” nor does it offer historical dynamics or institutional background of the discussion. The author rather intends to single out certain basic schemes and arguments that circulate in the discussions, place them in broader frames and anticipate their potential political consequences. Particularly, the text concentrates on various versions of the patriotic thinking that has been receiving broad popularity in today Russia. As the author is convinced, due familiarity with the contemporary Russian debate may help to better understand Russian internal as well as foreign policy nowadays: as it becomes clear, rise of patriotism in academia, society and politics are inseparable phenomena. Secondly, some of the arguments promoted by the contemporary Russian authors may enrich Western (non-Russian sovietology and debate on socialist experiments as such. Last but not least, both content and form of the Russian academic discussion convincingly point at cultural conditionality of social and historical sciences. To some extent the discussion reminds perpetual Russian debate on national identity; cardinally new context in which it takes place however justifies attention paid to it.

  20. Pracovní migrace a remitence. Současné trendy v době ekonomické krize

    Czech Academy of Sciences Publication Activity Database

    Stojanov, Robert; Strielkowski, Wadim; Drbohlav, D.

    2011-01-01

    Roč. 116, č. 4 (2011), s. 375-400 ISSN 1212-0014 R&D Projects: GA MŠk(CZ) ED1.1.00/02.0073 Institutional research plan: CEZ:AV0Z60870520 Keywords : international migration * remittances * economic crisis * imigration surplus Subject RIV: AO - Sociology, Demography Impact factor: 1.021, year: 2011

  1. Feast/famine regulatory proteins (FFRPs): Escherichia coli Lrp, AsnC and related archaeal transcription factors.

    Science.gov (United States)

    Yokoyama, Katsushi; Ishijima, Sanae A; Clowney, Lester; Koike, Hideaki; Aramaki, Hironori; Tanaka, Chikako; Makino, Kozo; Suzuki, Masashi

    2006-01-01

    Feast/famine regulatory proteins comprise a diverse family of transcription factors, which have been referred to in various individual identifications, including Escherichia coli leucine-responsive regulatory protein and asparagine synthase C gene product. A full length feast/famine regulatory protein consists of the N-terminal DNA-binding domain and the C-domain, which is involved in dimerization and further assembly, thereby producing, for example, a disc or a chromatin-like cylinder. Various ligands of the size of amino acids bind at the interface between feast/famine regulatory protein dimers, thereby altering their assembly forms. Also, the combination of feast/famine regulatory protein subunits forming the same assembly is altered. In this way, a small number of feast/famine regulatory proteins are able to regulate a large number of genes in response to various environmental changes. Because feast/famine regulatory proteins are shared by archaea and eubacteria, the genome-wide regulation by feast/famine regulatory proteins is traceable back to their common ancestor, being the prototype of highly differentiated transcription regulatory mechanisms found in organisms nowadays.

  2. Obraz současné ženy v moderní egyptské próze

    OpenAIRE

    Godiveau, Eliška

    2014-01-01

    The thesis examines the image of the modern Egyptian woman in the prose narrative of four contemporary women writers of Egypt. They are Hala El Badry, Salwa Bakr, Mona Prince and Miral al-Tahawy. Based on the interpretation and analysis of six selected literary texts this paper will aim at uncovering the authors' view on the woman of today's Egypt. The thesis will try to answer its research question, considering the historical development of woman's role and standing in Egyptian society, as w...

  3. Hodnotový svět současné mládeže

    OpenAIRE

    TOMÁNKOVÁ, Barbora

    2015-01-01

    This bachelor thesis deals with the issue of values, particularlywiththeviewof15-30 yearsoldyouthauthority questions. The theoretical part explains concepts such as youth, value, value systems and value orientation. It also focuses on family influence, the impact of school, and peer influence in current youth value systems.Furthermore, the theoretical part examines media and social networks, their influence on current young generation, and the role they play in youth´s lives. The practical pa...

  4. Kongres IFAC v Praze a některé současné problémy automatizace

    Czech Academy of Sciences Publication Activity Database

    Klán, Petr

    2005-01-01

    Roč. 11, č. 3 (2005), s. 86-88 ISSN 1210-9592 Institutional research plan: CEZ:AV0Z10300504 Keywords : IFAC Congress * modern automation * solved problems Subject RIV: BD - Theory of Information http://www.odbornecasopisy.cz/index.php?id_document=30405

  5. Vývoj techniky a taktiky tenisové hry, současné didaktické metody

    OpenAIRE

    Balík, Radek

    2011-01-01

    Title: The Development of Techniques and Tactics of Tennis, including Current Teaching Methods Summary: This bachelor thesis deals with the historical facts of tennis, charting the development of rackets, balls, and surfaces. Besides a brief description of the rules of tennis along with appropriate recommendations for beginners, the thesis covers the development of tennis techniques and tactics. In addition, previous training methods are compared with those of today and their regularities are...

  6. Současné modely likvidních finančních trhů

    OpenAIRE

    Grosu, Iulia

    2012-01-01

    The aim of this paper is to provide a comprehensive overview of selected current models of liquid financial markets, namely to explain their basic principles, assess their strengths and deficiencies and compare them with each other. The thesis is divided into six chapters. The first five chapters focus on the theoretical description of five different models, including their assumptions, the resulting consequences and, where possible, their mathematical formulation. The particular chapters als...

  7. Journal of Earth System Science | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    litharenite, wacke and quartz arenite. The corrected CIA values indicate that the weathering at source region was moderate to intense. The distribution of major and REE elements in the samples normalized to upper continental crust (UCC) and ...

  8. Guide for the self-assessment of risks brought upon patients in external radiotherapy - ASN guide nr 4, Index 0, Release of the 15/01/2009

    International Nuclear Information System (INIS)

    2009-01-01

    This document contains a user's guide for radiotherapy centres which is to be read before completing the form of analysis of failure modes, their effects and criticality (AMDEC), this form which must be completed by each radiotherapy centre, and a report explaining the methodology which has been adopted to elaborate this guide

  9. Hodnocení současné praxe tvorby designu obalů a návrhy na zlepšení

    OpenAIRE

    Lauermannová, Anna

    2008-01-01

    The thesis describes the packaging design theory. Then describes the czech market and packaging design. In the practical part there were interwieved 6 peoople from the czech enterprise market.At the end of the thesisi there is a packagafe o criterions of a goog packaging design accoarding to the czech experts.

  10. Politická kultura v narativech: kulturní repertoáry vztahování se k současné politice

    Directory of Open Access Journals (Sweden)

    Miroslav Grznár

    2014-12-01

    Full Text Available Political culture as a concept is an interdisciplinaryissue balancing between culturalanthropology, political science and sociology.This article presents conclusions of qualitativeresearch of contemporary czech political culture.Our research is based on 35 narrative interviewsconducted by students with their parents. We caninfer from quantitative studies that the level oftrust in the political system, institutions and actorshas hit the bottom. These studies are not ableto describe all aspects of political culture. Fromour position of interpretative paradigm we aimto analyse cultural repertoires through which isthe political situation narratively reconstructed.We perceive narrative as a performative genre ofrefering to social reality. We have identified thefollowing repertoires: 1. interested, 2. objective,3. evasive and 4. alienated. We have found fourtypes of layman’s theories, which were used bynarrators to explain the drop of trust in politics: 1.communist past 2. the nature of the Czech nation3. fatalistic theories of power 4. theories of “thesedays”.

  11. Porovnání současných a nových hašovacích funkcí

    OpenAIRE

    Suchan, Martin

    2007-01-01

    The goal of this study is to present comparison of today's most widely used cryptographic hash functions and compare them with drafts of new hash functions, which are being currently developed for Advanced Hash Standard competition. This study also includes implementation of all described functions in programming language C#.

  12. Současná obchůzková koleda na Smrtnou neděli v Čechách

    Czech Academy of Sciences Publication Activity Database

    Thořová, Věra

    č. 4 (2000), s. 160-176 ISSN 1212-8112 R&D Projects: GA AV ČR IAA0058904 Keywords : Carollings at the Death Sunday, in the spring pre-Easter time, the figure of Death Subject RIV: AC - Archeology, Anthropology, Ethnology

  13. Decision n. 2009-DC-0103 made on the 1. of July 2010 by the Nuclear Safety Authority (ASN) defining obligations of quality assurance in radiotherapy

    International Nuclear Information System (INIS)

    2008-01-01

    This document addresses various aspects and issues: the general requirements of a quality management system, the leadership commitment on this aspect, organisational provisions, the documentation system and its control, the personnel responsibility, the study of risks associated with the radiotherapy process for the patients, the internal declaration of dysfunctions and undesirable situations, improvement actions planning, internal communication, records. These terms and others are more precisely defined in an appendix

  14. Časná znalost jazyka: vývoj receptivní znalosti jazyka v prvních dvou letech života

    Czech Academy of Sciences Publication Activity Database

    Smolík, Filip

    2006-01-01

    Roč. 50, č. 3 (2006), s. 238-250 ISSN 0009-062X Institutional research plan: CEZ:AV0Z70250504 Keywords : knowledge and language * receptive knowledge of language * development of knowledge of language Subject RIV: AN - Psychology Impact factor: 0.279, year: 2006

  15. Osobnost typu C (cancer personality): současný stav poznatků a implikace pro další výzkum

    Czech Academy of Sciences Publication Activity Database

    Blatný, Marek; Adam, Z.

    2008-01-01

    Roč. 54, č. 6 (2008), s. 523-530 ISSN 0042-773X R&D Projects: GA ČR GA406/07/1384 Institutional research plan: CEZ:AV0Z70250504 Keywords : cancer * cancer-prone personality * methodology Subject RIV: AN - Psychology

  16. Vlčnovská jízda králů pohledem současného výzkumu (experimentu)

    Czech Academy of Sciences Publication Activity Database

    Stavělová, Daniela

    2013-01-01

    Roč. 23, č. 1 (2013), s. 3-14 ISSN 0862-8351 Institutional support: RVO:68378076 Keywords : Ride of the Kings * field research * methodology * festival * traditional custom * Vlčnov Subject RIV: AC - Archeology, Anthropology, Ethnology

  17. Místo waldorfské pedagogiky v současném českém školství

    OpenAIRE

    Franko, Radek

    2014-01-01

    This diploma thesis deals with Waldorf pedagogy. It is divided into five chapters. The first two map the circumstances of Waldorf education and life-shaping the mind constructs its founder Rudolf Steiner. The remaining three chapters answer questions regarding: Waldorf teaching specifics, personality profile Waldorf teachers and planting Czech Waldorf pedagogy in contemporary Czech education stream. Key words anthroposophy, Czech education, Rudolf Steiner, Waldorf school teacher, Waldorf peda...

  18. Různé podoby současné komunikace: mezi mluveností a psaností (2. část)

    Czech Academy of Sciences Publication Activity Database

    Hoffmannová, Jana

    2011-01-01

    Roč. 61, č. 4 (2011), s. 176-184 ISSN 0009-0786 R&D Projects: GA ČR GA405/09/2028 Institutional research plan: CEZ:AV0Z90610518 Keywords : spoken communication * written language * Czech Subject RIV: AI - Linguistics

  19. Příčiny a důsledky současné krize v automobilovém průmyslu

    OpenAIRE

    Valas, Jiří

    2009-01-01

    This diploma thesis describes the main causes of the automotive crisis in the first half of 2009 and their influence on this industry in the world. It also shows the effort of selected countries to fight these consequences and to support the demand for cars. One section is also devoted to situation in Czech Republic and to one of the Czech supplier to OEM car manufacturers.

  20. Odchylky od spisovné jazykové normy v současných právních textech

    Czech Academy of Sciences Publication Activity Database

    Smejkalová, Kamila; Prokšová, Hana

    2017-01-01

    Roč. 50, 7/8 (2017), s. 401-410 ISSN 0139-6005 Institutional support: RVO:68378092 Keywords : norm * codification * standard language * administrative style * orthography Subject RIV: AI - Linguistics OBOR OECD: Linguistics http://www.mvcr.cz/clanek/spravni-pravo-cislo-7-8-2017.aspx

  1. Physical stability comparisons of IgG1-Fc variants: effects of N-glycosylation site occupancy and Asp/Gln residues at site Asn 297.

    Science.gov (United States)

    Alsenaidy, Mohammad A; Okbazghi, Solomon Z; Kim, Jae Hyun; Joshi, Sangeeta B; Middaugh, C Russell; Tolbert, Thomas J; Volkin, David B

    2014-06-01

    The structural integrity and conformational stability of various IgG1-Fc proteins produced from the yeast Pichia pastoris with different glycosylation site occupancy (di-, mono-, and nonglycosylated) were determined. In addition, the physical stability profiles of three different forms of nonglycosylated Fc molecules (varying amino-acid residues at site 297 in the CH 2 domain due to the point mutations and enzymatic digestion of the Fc glycoforms) were also examined. The physical stability of these IgG1-Fc glycoproteins was examined as a function of pH and temperature by high-throughput biophysical analysis using multiple techniques combined with data visualization tools (three index empirical phase diagrams and radar charts). Across the pH range of 4.0-6.0, the di- and monoglycosylated forms of the IgG1-Fc showed the highest and lowest levels of physical stability, respectively, with the nonglycosylated forms showing intermediate stability depending on solution pH. In the aglycosylated Fc proteins, the introduction of Asp (D) residues at site 297 (QQ vs. DN vs. DD forms) resulted in more subtle changes in structural integrity and physical stability depending on solution pH. The utility of evaluating the conformational stability profile differences between the various IgG1-Fc glycoproteins is discussed in the context of analytical comparability studies. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

  2. Akce "Visła" - Zobrazení v propagandě lidového Polska a současné interpretace

    OpenAIRE

    Veselý, Luboš

    2006-01-01

    This thesis deals with expulsions of (ethnic) Ukrainians from their homeland in post- war Poland in 1944-1947 and the presentation of these in communist Poland's propaganda. It also analyses different interpretations of these events after the political changes in Central and Eastern Europe at the end of the 1980s. Post-war changes of Poland's eastern boarder, where the Soviet Union could keep the territories it had occupied in 1939, led to a situation where only 0,5 to 0,7 million Ukrainians ...

  3. Utváření vztahu rodičů k předčasně narozeným dětem

    Czech Academy of Sciences Publication Activity Database

    Chlebounová, M.; Čermák, Ivo

    2013-01-01

    Roč. 57, č. 4 (2013), s. 307-316 ISSN 0009-062X Institutional support: RVO:68081740 Keywords : preterm labor * premature infants * parenting * relationship formation * fatherhood Subject RIV: AN - Psychology Impact factor: 0.292, year: 2013

  4. Současné sociální změny, jejich důsledky a syndrom vyhoření

    Czech Academy of Sciences Publication Activity Database

    Kebza, V.; Šolcová, Iva

    2013-01-01

    Roč. 57, č. 4 (2013), s. 329-341 ISSN 0009-062X Institutional support: RVO:68081740 Keywords : burnout syndrome * unemployment * engagement * organizational civility Subject RIV: AN - Psychology Impact factor: 0.292, year: 2013

  5. Wuerzburská škola: reinterpretace jejích výsledků z pozic současné kognitivní psychologie

    Czech Academy of Sciences Publication Activity Database

    Sedláková, Miluše

    2001-01-01

    Roč. 7, č. 1 (2001), s. 23-55 ISSN 1211-216X Institutional research plan: CEZ:AV0Z7025918 Keywords : history of psychology * psychology of the 20th century * Wuerzburg's school Subject RIV: AN - Psychology

  6. Vliv časné imunitní stimulace a její odezva v animálním modelu schizofrenie

    Czech Academy of Sciences Publication Activity Database

    Tejkalová, H.; Kačer, P.; Růžičková, Š.; Klaschka, Jan; Horáček, J.

    2017-01-01

    Roč. 21, Suppl. 2 (2017), s. 53-55 ISSN 1211-7579 R&D Projects: GA ČR(CZ) GBP304/12/G069 Institutional support: RVO:67985807 Keywords : schizofrenie * imunitní systém * cytokiny * potkan * lipopolysacharid Subject RIV: FH - Neurology

  7. Historický a současný vývoj sesuvného území u Třebenic

    Czech Academy of Sciences Publication Activity Database

    Balek, Jan; Marek, Tomáš; Kadlečík, Pavel

    2015-01-01

    Roč. 2014, podzim (2015), s. 51-54 ISSN 0514-8057 Institutional support: RVO:67985891 Keywords : České středohoří Mts. * historical * current * landslide activity * monitoring Subject RIV: DE - Earth Magnetism, Geodesy, Geography

  8. Současná gastroturistika v Evropě: významné destinace a vývojové trendy

    OpenAIRE

    Kondratenko, Anna

    2013-01-01

    Nowadays there is a lot of new trends in a tourism sector and gastronomic tourism is one of the most important one. The object of thesis is to define the status of gastronomic tourism on tourist market. This object is achieved by introduction of the relations between tourism and gastronomy and by the classification of gastronomic tours. In this thesis the most visited countries of Europe are described and there is the analysis of the offer and demand on gastronomic tourism in these regions. A...

  9. Staročeská fotoarchivní sbírka. Historie, současný stav a význam

    Czech Academy of Sciences Publication Activity Database

    Rejzlová, Vendula

    2014-01-01

    Roč. 62, č. 1 (2014), s. 133-137 ISSN 0009-0468 Institutional support: RVO:68378068 Keywords : Czech literature * medieval literature * baroque literature * photo archive * repository * on-line catalogue * philological documentation Subject RIV: AJ - Letters, Mass-media, Audiovision

  10. Neolitické a časně eneolitické hroby z Prahy-Jihozápadního Města

    Czech Academy of Sciences Publication Activity Database

    Petriščáková, K.; Dobeš, Miroslav; Popelka, M.

    2016-01-01

    Roč. 33, 1-2 (2016), s. 335-349 ISSN 0231-5432 Institutional support: RVO:67985912 Keywords : Prague * Late Neolithic Period * Stroked Pottery Culture * Early Eneolithic Period * Jordanów Culture * graves Subject RIV: AC - Archeology, Anthropology, Ethnology OBOR OECD: Archaeology

  11. Marihuana a současný životní styl dětí a mládeže

    OpenAIRE

    KOBRLOVÁ, Kateřina

    2008-01-01

    The work follows adolescents and their relation to marihuana. In the theoretic part, marihuana is not considered just as a drug. Other ways of using it are mentioned there, for example, it can be used as an alternative renewable raw material for industry and economy. The research part is focused on concrete dates gaining about adolescents with an experience in smoking marihuana and confirming the truth of research tasks made according to the theoretic part. The work is generally aimed to prov...

  12. Felix Vodička v kontextu současné literární teorie a historie: recepce a revize

    Czech Academy of Sciences Publication Activity Database

    Sládek, Ondřej

    2010-01-01

    Roč. 92, 1/2 (2010), s. 95-105 ISSN 0862-8459. [100 let generace prvních žáků PLK. Praha, 09.02.2009-09.02.2009] Institutional research plan: CEZ:AV0Z90560517 Keywords : theory of literature * literary history * Vodička, Felix * structuralism * Prague School Subject RIV: AJ - Letters, Mass-media, Audiovision

  13. Zařazení notebooků do současných informačních systémů

    OpenAIRE

    Kubeš, Adam

    2009-01-01

    This bachelor work focuses especially on categorization of notebooks in ICT of present IS. It extends and fills in my previous semestral work called "Notebooks", that I was working on in course Technical means and infrastructure of IS. The work is divided into two main sections -- theoretical and practical. Theoretical section devotes to the description of present types of computers and categorization of notebooks in ICT, their use, contributions, limitations, energy management and synchroniz...

  14. Analýza současné situace na trhu elektronických knih (e-book) v České republice

    OpenAIRE

    Kubeš, Adam

    2011-01-01

    The thesis "Analysis of the current situation on the market of electronic books (eBook) in the Czech Republic", deals with topics and issues of e-books and the current market situation in the Czech Republic. One of its objectives is to introduce readers to the world of e-books, show a brief history and development of books and e-books as a medium, getting to know what's happening in the U.S., and particularly on the Czech market. For this thesis, it was developed an online questionnaire "Elec...

  15. Súčasný stav a perspektívy udržateľného rozvoja cestovného ruchu na Slovensku

    OpenAIRE

    Libantová, Silvia

    2015-01-01

    Tourism contributes to the prosperity of destinations by bringing financial resources, new jobs, a higher level of services and infrastructure development. However, if tourism does not develop sustainably, it is accompanied by negative factors, as well. Especially, negative impacts on the environment, such as: water and soil contamination, increased noise and overcrowded destination during the high season. As a response to the worsening state of the environment and possible irreversible chang...

  16. Časně eneolitická výšinná lokalita na Okrouhlíku v Praze-Libni

    Czech Academy of Sciences Publication Activity Database

    Dobeš, Miroslav

    2016-01-01

    Roč. 23, č. 1 (2016), s. 15-31 ISSN 0231-6390 Institutional support: RVO:67985912 Keywords : Early Eneolithic * hilltop settlement * Okrouhlík Subject RIV: AC - Archeology, Anthropology, Ethnology OBOR OECD: Archaeology

  17. „Politika 4. cenové skupiny”. K problematice současných politických klišé a stereotypů

    Czech Academy of Sciences Publication Activity Database

    Altman, Karel

    2016-01-01

    Roč. 16, č. 1 (2016), s. 70-84 ISSN 1506-5790 Institutional support: RVO:68378076 Keywords : Czechia * communication * political stereotypes * political clichés * Pub industries Subject RIV: AC - Archeology, Anthropology, Ethnology OBOR OECD: Antropology, ethnology

  18. Současná pojetí vztahu mezi krátkodobou pamětí a vědomím

    Czech Academy of Sciences Publication Activity Database

    Špok, Dalibor

    2003-01-01

    Roč. 47, č. 5 (2003), s. 424-436 ISSN 0009-062X Institutional research plan: CEZ:AV0Z7025918 Keywords : Short-term memory * working memory * consciousness Subject RIV: AN - Psychology Impact factor: 0.232, year: 2003

  19. Si3N4/Si/In0.05Ga0.95As/n endash GaAs metal endash insulator endash semiconductor devices

    International Nuclear Information System (INIS)

    Park, D.; Li, D.; Tao, M.; Fan, Z.; Botchkarev, A.E.; Mohammad, S.N.; Morkoc, H.

    1997-01-01

    We report a novel metal endash insulator endash semiconductor (MIS) structure exhibiting a pseudomorphic In 0.05 Ga 0.95 As layer on GaAs with interface state densities in the low 10 11 eV -1 cm -2 . The structure was grown by a combination of molecular beam epitaxy and chemical vapor deposition methods. The hysteresis and frequency dispersion of the MIS capacitor were lower than 100 mV, some of them as low as 30 mV under a field swing of about ±1.3 MV/cm. The 150-Angstrom-thick In 0.05 Ga 0.95 As channel between Si and GaAs is found to bring about a change in the minority carrier recombination behavior of the GaAs channel, in the same way as done by In 0.53 Ga 0.47 As channel MIS structures. Self-aligned gate depletion mode In 0.05 Ga 0.95 As metal endash insulator endash semiconductor field-effect transistors having 3 μm gate lengths exhibited field-effect bulk mobility of 1400 cm 2 /Vs and transconductances of about 170 mS/mm. copyright 1997 American Institute of Physics

  20. DGT/ASN circular n. 04 of the 21. of April 2010 related to provisions for the prevention to ionizing ray exposure risks

    International Nuclear Information System (INIS)

    2010-01-01

    After an overview of the context of risk of exposure to ionizing radiation (potentially concerned activities, prevention policy based on radioprotection principles, regulation origin, radioprotection organization, specific inspection), this document presents the different regulatory provisions for the protection of workers against ionizing radiation hazards (scope of application, risk assessment, technical rules for workspace design, collective and individual protections, conditions for the follow-up of exposed workers, medical follow-up, work abnormal condition, radioprotection operating organisation, exposure to natural radioactivity). Then, a set of sheets is proposed, respectively containing regulatory information on employer obligations in terms of radioprotection, technical controls of radioprotection, individual protection equipment, worker information and education, radiological follow-up of classified workers, worker medical follow-up, work abnormal conditions, person with abilities in radioprotection, and a list of application decisions and orders

  1. Sociální sítě jako nástroj současného marketingu

    OpenAIRE

    Kononova, Anna

    2017-01-01

    This bachelor thesis is primarily oriented on practical use of social networks in marketing. The main aim is to analyse the contribution of this modern platform to doing business and evaluate its potential for the future. Theoretical part introduces the mapping of the current position of social networks in marketing and opportunities for implementation of traditional marketing tools into this platform and provides an overview of the main advantages and disadvantages, that are consequent upon ...

  2. Percepce Bosny a Hercegoviny očima současných fotbalových příznivců

    OpenAIRE

    Dolejš, Jan

    2015-01-01

    (in English): The main objective of this thesis is to describe the Bosnian football environment, which is one of the mirrors of the ongoing social processes. On the basis of the interviews held between March and June 2014, the thesis seeks to interpret an everyday reality of Bosnia and Herzegovina from the perspective of football fans. Moreover, the thesis provides the picture of the past and present of Bosnia and Herzegovina, theoretically defines a football audience (worldwide and in the su...

  3. Syndrom vyhoření – rekapitulace současného stavu poznání a perspektivy do budoucna

    Czech Academy of Sciences Publication Activity Database

    Kebza, V.; Šolcová, Iva

    2008-01-01

    Roč. 52, č. 4 (2008), s. 351-365 ISSN 0009-062X R&D Projects: GA ČR GA406/06/0747 Institutional research plan: CEZ:AV0Z70250504 Keywords : burnout syndrome * vital exhaustion * engagement Subject RIV: AN - Psychology Impact factor: 0.101, year: 2008

  4. K otázce (ne)existence akuzativního se v současné češtině

    Czech Academy of Sciences Publication Activity Database

    Veselý, Luboš

    2013-01-01

    Roč. 5, č. 4 (2013), s. 308-317 ISSN 1803-876X R&D Projects: GA ČR GA13-07138S Institutional support: RVO:68378092 Keywords : reflexive * word form se * accusative case * enclitics * pronoun Subject RIV: AI - Linguistics

  5. Současné tetování z hlediska „rituálního“ jednání

    Directory of Open Access Journals (Sweden)

    Alena Kajanová

    2012-08-01

    Full Text Available Tattooing is one of the most widely spread forms of body modification in contemporary Czech society. The goal of this article is to inquire into contemporary practices of tattooing with regard to their “ritual” functions. Our research question was whether ritual functions attached to tattooing still remain, or whether they have already been entirely substituted by purely aesthetic functions. We were further interested in the motivations leading to the decision of getting a tattoo and their relation to certain time periods, such as crises. Is obtaining a tattoo a response to a crisis, or rather to the fact that it has been overcome? Our sample consists of sixteen respondents wearing at least two tattoos. The respondent’s narrations are supplemented by the perspectives of professional tattoo artists. Our data analysis suggests that among both the tattooed and tattoo artists, tattoo art is perceived rather in its aesthetic and individualizing functions than in its social-ritual function. For the tattooed themselves, tattooing carries deep meanings attached to the process of their (social individualization. They perceive the very process of tattooing, accompanied by considerable pain, as a form of individualized rite of passage. For some of the tattooed, the new tattoo concludes a previous period of life, while opening a new one, into which they emerge as new beings.

  6. Inspirace současného managementu strategickými prvky používanými ve vojenství

    OpenAIRE

    Schubert, Petr

    2008-01-01

    In today's globalised hypercompetition dominated world, companies are often fighting for survival. This theses tries to verify, if a way out could be found in the usage of strategic elements used in military approaches. Inspiration is sought in Sunzi ("The Art of War"), Carl von Clausewitz ("On War") and Nicollo Machiavelli ("The Prince"). The company's goal is to generate profit and managers should aim to achieve this goal by using every available information and opportunity, preferably with...

  7. Potenciální mikrobiologické nebezpečí v současných trendech kosmetiky - bio, vegan, raw.

    OpenAIRE

    Jančíková, Simona

    2017-01-01

    Bio-, vegan- a raw-kosmetika patří k stále oblíbenějším druhům kosmetických produktů z důvodu absence syntetických látek a konzervantů, z tohoto hlediska se ale zvyšuje pravděpodobnost mikrobiální kontaminace. Předmětem práce je tedy především praktické zjištění potenciální mikrobiologické kontaminace pomocí zátěžového testu a také stanovení aktivních složek v kosmetických přípravcích. V teorii byla zpracována legislativa kosmetických přípravků, a také informace o certifikátech, které se pro ...

  8. Elaboration and modification of the waste zoning plan in basic nuclear installations. ASN Guide Nr 23, Release of the 2016/08/30

    International Nuclear Information System (INIS)

    2016-01-01

    After having recalled the relevant regulatory texts and guides, this guide aims at detailing conditions of elaboration and of modification of the waste zoning plan which is defined in one of these texts, and applies requirements and recommendations of others of them. It first recalls the general doctrine of waste management in basic nuclear installations. It describes how the waste zoning plan and the reference waste zoning map are elaborated and justified, how the relevancy of this plan and this map are confirmed through radiological controls and based on general operation rules and periodic assessments. It also describes how contamination transfers and material activation are prevented, and discusses waste radiological controls to be performed. The next part addressed waste zoning and ways of management for wastes coming from conventional waste areas, and from areas of possible production of nuclear wastes. Issues of waste zoning downgrading or reclassification are then addressed by distinguishing temporary and definitive modifications (in one way or the other). Some peculiar cases are addressed: delimitation of waste zoning at the level of equipments, conventional processing of some wastes, radioactive waste warehousing, outdoor areas of possible production of nuclear wastes

  9. Obraz własnego ciała współczesnej młodzieży a kultura popularna. Badania własne

    OpenAIRE

    Gajtkowska, Magdalena

    2013-01-01

    The author presents the outcome of her own research on the in uence of the pop culture on self-body image. In the research, di erent aspects of self-body image are taken into account: being t, healthy and being goodlooking or pretty. The analysis of the scienti c research persuades the reader that mass media are important but not the most important source of information which they use to judge? their bodies. Moreover, it is claimed that the meaning of cocalled pop culture for teenager...

  10. Časně eneolitické příkopové ohrazení v Trubíně, okr. Beroun

    Czech Academy of Sciences Publication Activity Database

    Dobeš, Miroslav; Křivánek, Roman; Kyselý, René; Stolz, D.

    2018-01-01

    Roč. 70, č. 1 (2018), s. 3-38 ISSN 0323-1267 Grant - others:AV ČR(CZ) R300021703 Institutional support: RVO:67985912 Keywords : Bohemia * Proto-Eneolithic * Jordanów culture * Schussenried phase * causewayed enclosure * radiocarbon dating Subject RIV: AC - Archeology, Anthropology, Ethnology OBOR OECD: Archaeology

  11. Časný a starší eneolit Kadaňska a horního Poohří pohledem kamenné industrie

    Czech Academy of Sciences Publication Activity Database

    Eigner, J.; Přichystal, A.; Dobeš, Miroslav

    2017-01-01

    Roč. 21, č. 2 (2017), s. 577-615 ISSN 1214-3553 Institutional support: RVO:67985912 Keywords : Eneolithic * Kadaň region * lithic industry Subject RIV: AC - Archeology, Anthropology, Ethnology OBOR OECD: Archaeology

  12. "Vlastní laboratoř": akademické trajektorie a gender v současných biovědách

    Czech Academy of Sciences Publication Activity Database

    Linková, Marcela; Červinková, Alice

    2013-01-01

    Roč. 14, č. 1 (2013), s. 15-26 ISSN 1213-0028 R&D Projects: GA MŠk(CZ) LE12003 Institutional support: RVO:68378025 Keywords : biosciences * dynamic labs * excellence Subject RIV: AO - Sociology, Demography

  13. Gesta v současné španělštině a jejich role v jazykové komunikaci

    OpenAIRE

    Johánková, Tereza

    2008-01-01

    This diploma thesis focuses on gestures in contemporary Spanish and their role in linguistic communication. It contains the definitions of basic terms, the introduction, five major parts, the conclusion and five appendices with pictures and charts. Some of the appendices include videos. Since this diploma work does not concern only gestures but also communication, its first part concentrates on language, communication and their mutual relationship. First, language, its division, types, functi...

  14. Vidění nás klame: Problematika pravdy a reality v současné dokumentární tvorbě

    OpenAIRE

    Radostová, Kristýna

    2014-01-01

    This thesis examines the issue of depicting reality and truth in contemporary documentary. Documentaries are often considered to be a mirror to the real world, but this is not always the case. Filmmakers can manipulate their work in a manner that changes the story to be more interesting for viewers. This thesis introduces a documentary discourse, provides contexts of documentary history in the 20th Century and looks more closely at issues of depicting reality and truth. This is shown through ...

  15. Kulturní relativismus: jeho filosofické kořeny, současné debaty a kritické zhodnocení

    OpenAIRE

    RYBOVÁ, Nela

    2016-01-01

    The work deals with topic of cultural relativism. The first chapter is concerned with concept of cultural relativism, its understanding, definitions, its connection with other conceptions of relativism, i.e. ethical and cognitive relativism. The second part concentrates on finding of philosophical roots, which became the fundamental presumptions for origin of cultural relativism, whereas the second part contains the chapter about history of cultural anthropology, which treats of establishment...

  16. Harmonizace pracovního a rodinného života současné české společnosti

    OpenAIRE

    SOUKUPOVÁ, Barbora

    2010-01-01

    Over the last two decades, there has been many political, social, economical and cultural alterations that have brought a number of changes to the fabric of Czech society, equally effecting the workplace and family life. The aim of my work is to give a sociological review using my completed overview studies that reflect upon the consequences of these changes and mutually bind them between the workplace and family life. Using appropriate questions, the basis of my study is to discover how curr...

  17. 76 FR 66187 - Bacteriophage of Clavibacter Michiganensis Subspecies Michiganensis; Exemption From the...

    Science.gov (United States)

    2011-10-26

    ... history of bacteriophage laboratory and pesticidal usage, adverse reports in the literature have not been... cheese factory in Argentina. Journal of Dairy Science 89:3791-3799. 19. Guillaumes J, Houdeau G, Germain...

  18. Raamaturappija / [Sinijärv, Karl Martin

    Index Scriptorium Estoniae

    Sinijärv, Karl Martin, 1971-

    1997-01-01

    Vandenberg, Philipp. Viies evangeelium; Fischer, Hans. Saatuslik armastus majas nr. 34; Bulgakov, Mihhail. Meister ja Margarita; Paju, Juhan. Katkenud romaan; Duby, Georges. Guillaume le Maréchal ehk Maailma parim rüütel

  19. Bernoulli Brothers

    Indian Academy of Sciences (India)

    In the long, glorious history of mathematics, we come across ... family flourished in business, but afterwards, its descendants .... teaching calculus to contemporary renowned mathematicians including Guillaume FrancoiseAntoiRe de.

  20. Feedback on the significant events of radiation protection declared to Asn in external radiotherapy, from necessity of a quality management to the service of risk management; Retour d'experience sur les evenements significatifs de radioprotection declares a l'asn en radiotherapie externe, de la necessite d'un management de la qualite au service de la gestion des risques

    Energy Technology Data Exchange (ETDEWEB)

    Marchal, C. [ASN, 75 - Paris (France); Garandel, S.; Godet, J.L. [Angers Univ., 49 (France)

    2009-10-15

    The declaration of events in the perspective of a experience feedback towards the professionals contributes to improve the treatments safety. The stakes in term of treatments safety is in the capitalization of these experience feedbacks from declarations. Well, the publication of regulatory obligations in matter of quality assurance and their appropriation by the whole of medical teams should improve the treatment safety and and increase their quality. To the end, without being unaware of the current difficulties encountered y some centers because of the lack of radio physicists, the approach of significant events declaration and risk analysis should allow to the french radiotherapy to become an international reference. (N.C.)

  1. Radioprotection optimization in the electronuclear, industrial and medical domains

    International Nuclear Information System (INIS)

    Schieber, C.; Abela, G.; Ammerich, M.; Balduyck, S.; Batalla, A.; Drouet, F.; Fracas, P.; Gauron, Ch.; Le Guen, B.; Lombard, J.; Mougnard, Ph.; Murith, Ch.; Rannou, A.; Rodde, S.; Selva, M.; Tranchant, Ph.; Schieber, C.; Solaire, T.; Le Tonqueze, Y.; Jolivet, P.; Chauveau, D.; Mathevet, L.; Juhel, T.; Mertz, L.; Bochud, F.O.; Desmaris, G.; Turquet de Beauregard, G.; Roy, C.; Delacroix, S.; Sevilla, A.; Rehel, J.L.; Bernhard, S.; Palut-Laurent, O.; Lochard, J.; Lebaron-Jacobs, L.; Wack, G.; Barange, K.; Delabre, H.

    2011-01-01

    (S. Delacroix); 20 - optimisation of operational extremity dosimetry using video (A. Sevilla); 21 - optimization in interventional radiology: uterine embolization (J.L. Rehel); 22 - optimization of radon-linked radioprotection in dwellings (C. Murith); 23 - NORMS companies prospects about optimization implementation (S. Bernhard); 24 - management of sites and soils polluted by radioactive compounds: synthesis of the works made by the pluralist thinking group on the approach to implement for the rehabilitation of radio-contaminated sites (O. Palut-Laurent); 25 - new stakes of radioprotection optimization: CIPR's publications no. 101 and 103 (J. Lochard); 26 - place of optimization in the new basic safety standards (BSS) of the European Commission (J. Lebaron-Jacobs); 27 - ASN's point of view on ALARA's consideration (G. Wack); 28 - initiatives related to radioprotection culture (B. Le Guen); 29 - training as optimization tool (K. Barange); 30 - networks as spreading relays for radioprotection training and culture (A. Batalla); 31 - the radio-protectionists' club (H. Delabre and L. d'Ascenzo). (J.S.)

  2. Radioprotection optimization in the electronuclear, industrial and medical domains; Optimisation de la radioprotection dans les domaines electronucleaire, industriel et medical

    Energy Technology Data Exchange (ETDEWEB)

    Schieber, C.; Abela, G.; Ammerich, M.; Balduyck, S.; Batalla, A.; Drouet, F.; Fracas, P.; Gauron, Ch.; Le Guen, B.; Lombard, J.; Mougnard, Ph.; Murith, Ch.; Rannou, A.; Rodde, S.; Selva, M.; Tranchant, Ph.; Schieber, C.; Solaire, T.; Le Tonqueze, Y.; Jolivet, P.; Chauveau, D.; Mathevet, L.; Juhel, T.; Mertz, L.; Bochud, F.O.; Desmaris, G.; Turquet de Beauregard, G.; Roy, C.; Delacroix, S.; Sevilla, A.; Rehel, J.L.; Bernhard, S.; Palut-Laurent, O.; Lochard, J.; Lebaron-Jacobs, L.; Wack, G.; Barange, K.; Delabre, H.

    2011-07-01

    Institute (S. Delacroix); 20 - optimisation of operational extremity dosimetry using video (A. Sevilla); 21 - optimization in interventional radiology: uterine embolization (J.L. Rehel); 22 - optimization of radon-linked radioprotection in dwellings (C. Murith); 23 - NORMS companies prospects about optimization implementation (S. Bernhard); 24 - management of sites and soils polluted by radioactive compounds: synthesis of the works made by the pluralist thinking group on the approach to implement for the rehabilitation of radio-contaminated sites (O. Palut-Laurent); 25 - new stakes of radioprotection optimization: CIPR's publications no. 101 and 103 (J. Lochard); 26 - place of optimization in the new basic safety standards (BSS) of the European Commission (J. Lebaron-Jacobs); 27 - ASN's point of view on ALARA's consideration (G. Wack); 28 - initiatives related to radioprotection culture (B. Le Guen); 29 - training as optimization tool (K. Barange); 30 - networks as spreading relays for radioprotection training and culture (A. Batalla); 31 - the radio-protectionists' club (H. Delabre and L. d'Ascenzo). (J.S.)

  3. Současné možnosti výroby octa a jeho uplatnění v potravinářství

    OpenAIRE

    Rumíšková, Soňa

    2014-01-01

    The aim of this thesis was to develop a literature review on the topic: Technology of vinegar. Vinegar can be made by several methods; these methods are explained in the thesis. The most widely used method nowadays is the submerged method which is also the most modern methods. Raw materials used for the vinegar production are discussed in next section; special attention is paid to ethanol as the main raw material. Then the thesis focuses on factors that may affect the formation of acetic acid...

  4. Súčasné trendy a rozvoj technológií v stravovacích službách v leteckej doprave

    OpenAIRE

    Flamíková, Dominika

    2012-01-01

    The main objective of this diploma thesis is to analyze current trends in the airline catering. This thesis also focuses on the latest technologies developed for catering services in aviation. Important are also the results of a survey of the author of this thesis. The main task of the survey was to determine the importance of the airline catering for passengers, and thus for the aviation industry as a whole.

  5. Jazyková a kulturní různorodost v současné české komerční reklamě

    Czech Academy of Sciences Publication Activity Database

    Mrázková, Kamila

    2015-01-01

    Roč. 24, č. 1 (2015), s. 319-329 ISSN 1230-2287 R&D Projects: GA ČR GAP406/12/1829 Institutional support: RVO:68378092 Keywords : advertising discourse * advertising style * multilingualism * loanwords * English as the language of globalization Subject RIV: AI - Linguistics

  6. Současná česká kultura (styl básnických a dramatických textů) a kultura/kultivovanost jazyka/řeči

    Czech Academy of Sciences Publication Activity Database

    Hoffmannová, Jana

    2012-01-01

    Roč. 21, č. 1 (2012), s. 83-96 ISSN 1230-2287 R&D Projects: GA ČR GAP406/12/1829 Institutional support: RVO:68378092 Keywords : language culture * speech cultivation * style * expressive means Subject RIV: AI - Linguistics

  7. Křemíkové solární články - současná výroba a připravované (nano) technologické trendy

    Czech Academy of Sciences Publication Activity Database

    Poruba, Aleš; Buřinka, R.; Vaněček, Milan

    2012-01-01

    Roč. 106, č. 6 (2012), s. 511-512 ISSN 0009-2770 R&D Projects: GA MŠk(CZ) 7E09057 EU Projects: European Commission(XE) 214134 - N2P Institutional research plan: CEZ:AV0Z10100521 Keywords : crystalline Si solar cells * efficiency * 3D design Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 0.453, year: 2012 http://www.chemicke-listy.cz/docs/full/2012_06_509-514.pdf

  8. Současný stav a možnosti řešení dětského kuřáctví

    OpenAIRE

    VÁVROVÁ, Eva

    2008-01-01

    The paper has theoretical and practical part. The theoretical part involves information on smoking history, substances contained in cigarettes and it also focuses on smoking impacts to children health, adults, pregnant women and human fertility. Other sections focus on smoking and related legislation, a theory of addiction development and its treatments. The theoretical part ends with the detailed description of a juvenile smoking phenomenon focusing on prevention schemes, how to prevent chil...

  9. Komparace íránské a ruské revoluce v dílech dvou současných exilových íránských autorů

    Directory of Open Access Journals (Sweden)

    Marek Německý

    2014-06-01

    Full Text Available Article describes the books of two authors, who try to compare Russian and Iranian revolutions and political development generally. Whereas Ghoncheh Tazmini uses Hegelian structuralist approach, that sees these revolutions as an inevitable result of modernization from above, another Iranian political scientist Zhand Shakibi puts stress on the role of human actor – pre-revolutionary monarch and his (missmanagement of the situation in explaining the origins of revolution. Shakibi applies the human agency perspective to the analysis of the character of pre-revolutionary monarch and his modus operandi. According to Shakibi the structural variables did not by themselves cause the revolution, they create only potential for revolution. The revolutions must be explained by describing complex interactions between structural variables and modus operandi of the monarchs.

  10. Post-Fukushima complementary safety assessments. Information note on the IRSN analysis and conclusions after the expertise of Complementary Safety Assessments (ECS) reports handed to the ASN by operators, on the request of the Prime Minister, after the Fukushima accident

    International Nuclear Information System (INIS)

    2011-01-01

    This note first states the three main conclusions of the analysis performed by the IRSN of ECS (complementary safety assessments) reports made after the Fukushima accident. The IRSN noticed some biases of conformity between installations, outlines the need to make installation safety referential evolve, and that the idea that an extreme natural phenomenon could not result in a severe accident must be put into question again. Then, the report describes why and how nuclear installation safety 'hard cores' must be created in order to avoid the occurrence of incidental or accidental situations, or to implement measures aimed at handling these situations. It finally outlines the outcomes of the ECSs for the IRSN

  11. Současný výskyt epilepsie zhoršuje některé příznaky poruch autistického spektra

    Czech Academy of Sciences Publication Activity Database

    Blatný, Marek; Urbánek, Tomáš; Kulísek, R.; Hrdlička, M.; Propper, L.; Komárek, V.; Zumrová, A.; Faladová, L.

    2003-01-01

    Roč. 47, č. 2 (2003), s. 97-104 ISSN 0009-062X Institutional research plan: CEZ:AV0Z7025918 Keywords : childhood autism * pervasive developmental disorders * epilepsy Subject RIV: AN - Psychology Impact factor: 0.232, year: 2003

  12. Využití umělých a poloumělých výtěrů v současném chovu kapra

    OpenAIRE

    MARKOVÁ, Aneta

    2014-01-01

    This thesis is focused on the use of artificial and semi-artificial spawning substrates in the current breeding of common carp (Cyprinus carpio, L.) a literature review of this species was processed in relation to reproduction, as well as of the spawning methods (natural, semi-artificial, artificial). Results are drawn from the experimental part (2 spawns: a semi-artificial spawn in the institution for experiments at the Fishery School in Vodňany and an artificial spawn in the fish hatchery a...

  13. Současné a budoucí trendy světového obchodu a těžby břidlicového plynu

    OpenAIRE

    Brückler, Jaroslav

    2014-01-01

    This thesis look into extraction and trade of shale gas. The aim of this thesis is to simply explain to the readers the basic principles of trade with the shale gas and to present its possibilities in extraction and its use. I also describe all side affects, that are related to the extraction and trade with the shale gas such as, impact on the environment or the price of the natural gas. The thesis provides the reader with the basic knowledge of processing of the shale gas, possibilities of i...

  14. Přinese současná diskuse o rovnosti odměňování změny v Evropské legislativě?

    Czech Academy of Sciences Publication Activity Database

    Formánková, Lenka

    2009-01-01

    Roč. 10, č. 2 (2009), s. 74-76 ISSN 1213-0028 R&D Projects: GA ČR GA403/09/1839 Institutional research plan: CEZ:AV0Z70280505 Keywords : Gender pay gap * EU legislation * gender inequalities Subject RIV: AO - Sociology, Demography http://www. gender online.cz

  15. Historie a současný stav urychlovačů částic v českých zemích. II. Kruhové urychlovače

    Czech Academy of Sciences Publication Activity Database

    Janovský, I.; Šimáně, Čestmír

    2012-01-01

    Roč. 62, č. 4 (2012), s. 239-247 ISSN 0009-0700 Institutional support: RVO:61389005 Keywords : accelerator history * cyclic accelerator s Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders

  16. Return on experience of the post-accidental management of the Chernobyl accident within the Belarusian context - PAREX. Synthesis report delivered to the ASN on the 19 March 2007

    International Nuclear Information System (INIS)

    Heriard Dubreuil, Gilles; Lochard, Jacques; Ollagnon, Henry; Baude, Stephane; Bataille, Celine

    2007-01-01

    This approach to a return on experience of the post-accidental management of the Chernobyl accident identified four main steps: the passage from an accidental phase to a post-accidental phase from 1986 to 1991 (implementation of countermeasures, design of a radiological protection), the setting of an institutional and legal framework for the post-accidental management from 1991 to 1993 (compensations, definition of standards for food production and consumption, psychological support of populations), the search for alternative strategies from 1993 to 2001, and a sustainable rehabilitation of living conditions in contaminated territories between 2001 and 2006. Based on the return on experience on this various issues, the authors draw a set of lessons and give recommendations. They notably address the characteristics of a situation of durable radiological contamination, the role of standards in the response to a post-accidental situation, the role of expertise and knowledge building, the role and action of public authorities, the progressive building up of a local response to the crisis, the preparedness to a situation of durable contamination

  17. Výsledky nového průzkumu časně laténských mečů z Chlumu u Rokycan a z Vlčí, okr. Plzeň-jih

    Czech Academy of Sciences Publication Activity Database

    Sankot, P.; Fořt, M.; Vopálenský, Michal; Kumpová, Ivana; Vavřík, Daniel

    2017-01-01

    Roč. 12, č. 1 (2017), s. 93-102 ISSN 1804-2953 R&D Projects: GA MŠk(CZ) LO1219 Keywords : Bohemia * La Tene culture * swords * conservation * computed tomography Subject RIV: AL - Art, Architecture, Cultural Heritage OBOR OECD: Archaeology

  18. Pojmenování barev a jejich odstínů v Akademickém slovníku současné češtiny

    Czech Academy of Sciences Publication Activity Database

    Lišková, Michaela; Pernicová, Helena

    2015-01-01

    Roč. 97, č. 2 (2015), s. 169-178 ISSN 0008-7386 R&D Projects: GA MK DF13P01OVV011 Keywords : monolingual dictionary * explanation of the meaning * compound adjectives * names of colours Subject RIV: AI - Linguistics http://bit.ly/1MBb0Pm

  19. Současný vývoj české historické balkanistiky. Bilance knižní produkce za léta 2005-2016

    Czech Academy of Sciences Publication Activity Database

    Hladký, Ladislav

    2017-01-01

    Roč. 103, č. 2 (2017), s. 455-468 ISSN 0037-6922 Institutional support: RVO:67985963 Keywords : Balkan studies * historiography * monographs * Czech republic * 2005-2016 Subject RIV: AB - History OBOR OECD: History (history of science and technology to be 6.3, history of specific sciences to be under the respective headings)

  20. HR Outsourcing - využití na současném trhu práce (případ středně velké společnosti)

    OpenAIRE

    Moravcová, Hana

    2009-01-01

    This master thesis discusses the possibilities of outsourcing (in Czech also displacement of activities) in a human resources area, it also considers its forms and applications in companies. The main goal is to determine whether and to what extent HR outsourcing is worth to be implemented into small to medium sized companies and what steps are needed to be done to benefit from HR outsourcing. The work is divided into two parts. The first part is based on theoretical foundations. It contains a...

  1. Dějiny lesa a lesnictví - variabilita přístupů a současné hlavní badatelské směry

    Czech Academy of Sciences Publication Activity Database

    Woitsch, Jiří

    2010-01-01

    Roč. 25, č. 2 (2010), s. 199-226 ISSN 0231-7540 Institutional research plan: CEZ:AV0Z90580513 Keywords : history of forestry * theory and methodology of forest historiography * changes of paradigms Subject RIV: AC - Archeology, Anthropology, Ethnology

  2. Současné trendy v komerčních komunikacích se zaměřením na nákupní cyklus.

    OpenAIRE

    Nováková, Markéta

    2010-01-01

    The thesis explains the term the commercial communication, especially parts of the communication mix. But mainly it analyzes contemporary situation in the commercial communication, which changes are happening and it also describes trends in the adressing the customers. One chapter is dedicated to the Purchasing cycle which is a product of the McCann-Erickson advertising agency.

  3. Analýza vizuální komunikace na příkladu současných evropských divadelních plakátů

    OpenAIRE

    Jonášová, Helena

    2014-01-01

    The aim of the diploma thesis is to describe and define basic principles of visual communication from the point of view of mass communication theory and classical media theory and to attempt to create a basic synthesis of these branches. The visual communication is currently dominating form of communication not only in advertising and marketing, but also from the point of purely artistic expressing, which nowadays abandons classical conservative techniques and searches for new ways of express...

  4. Analýza současného stavu a trendů v distribučních cestách na německém trhu

    OpenAIRE

    Zabloudilová, Pavlína

    2013-01-01

    The objective of this master's thesis is to analyse trends in distribution in the German market and evaluate their importance in the retail environment. The first chapter of the thesis deals with the issue of distribution from a theoretical perspective. Analysis of the German economy and retail environment serves as a basis for further elaboration of the thesis. In the German market is growing the importance of the e-commerce and there is a tendency of integration of distribution channels. Th...

  5. Paradox „opia lidu“. Náboženství a (ne)spokojenost v současné české společnosti

    Czech Academy of Sciences Publication Activity Database

    Nešpor, Zdeněk

    2013-01-01

    Roč. 100, č. 4 (2013), s. 409-425 ISSN 0009-0794 R&D Projects: GA ČR(CZ) GAP404/11/1521 Institutional support: RVO:68378025 Keywords : religion * happiness * satisfaction Subject RIV: AO - Sociology, Demography Impact factor: 0.094, year: 2012

  6. Analýza některých současných teorií celoživotního vývoje

    Czech Academy of Sciences Publication Activity Database

    Millová, Katarína

    2009-01-01

    Roč. 3, č. 4 (2009), s. 45-54 ISSN 1802-8853 Institutional research plan: CEZ:AV0Z70250504 Keywords : lifespan development * theories * developmental science Subject RIV: AN - Psychology http://e-psycholog.eu/pdf/millova.pdf

  7. Missense mutation Lys18Asn in dystrophin that triggers X-linked dilated cardiomyopathy decreases protein stability, increases protein unfolding, and perturbs protein structure, but does not affect protein function.

    Directory of Open Access Journals (Sweden)

    Surinder M Singh

    Full Text Available Genetic mutations in a vital muscle protein dystrophin trigger X-linked dilated cardiomyopathy (XLDCM. However, disease mechanisms at the fundamental protein level are not understood. Such molecular knowledge is essential for developing therapies for XLDCM. Our main objective is to understand the effect of disease-causing mutations on the structure and function of dystrophin. This study is on a missense mutation K18N. The K18N mutation occurs in the N-terminal actin binding domain (N-ABD. We created and expressed the wild-type (WT N-ABD and its K18N mutant, and purified to homogeneity. Reversible folding experiments demonstrated that both mutant and WT did not aggregate upon refolding. Mutation did not affect the protein's overall secondary structure, as indicated by no changes in circular dichroism of the protein. However, the mutant is thermodynamically less stable than the WT (denaturant melts, and unfolds faster than the WT (stopped-flow kinetics. Despite having global secondary structure similar to that of the WT, mutant showed significant local structural changes at many amino acids when compared with the WT (heteronuclear NMR experiments. These structural changes indicate that the effect of mutation is propagated over long distances in the protein structure. Contrary to these structural and stability changes, the mutant had no significant effect on the actin-binding function as evident from co-sedimentation and depolymerization assays. These results summarize that the K18N mutation decreases thermodynamic stability, accelerates unfolding, perturbs protein structure, but does not affect the function. Therefore, K18N is a stability defect rather than a functional defect. Decrease in stability and increase in unfolding decrease the net population of dystrophin molecules available for function, which might trigger XLDCM. Consistently, XLDCM patients have decreased levels of dystrophin in cardiac muscle.

  8. Historické knižní fondy v České republice a současný stav jejich knihovnického zpracování

    Czech Academy of Sciences Publication Activity Database

    Baďurová, Anežka

    2003-01-01

    Roč. 53, č. 2 (2003), s. 641-684 ISSN 0036-5246 Institutional research plan: CEZ:AV0Z7083919 Keywords : historical book collections * historical bibliography * book culture Subject RIV: AF - Documentation, Librarianship, Information Studies

  9. Meteorologické stožáry– historie, účel a současné využití v České republice

    Czech Academy of Sciences Publication Activity Database

    Komínková, Kateřina; Dvorská, Alice; Prošek, P.

    2015-01-01

    Roč. 68, č. 5 (2015), s. 148-152 ISSN 0026-1173 R&D Projects: GA MŠk(CZ) LO1415 Institutional support: RVO:67179843 Keywords : Křešín u Pacova Atmospheric Station * measurement of meteorological elements * ozone * gaseous elemental mercury * greenhouse gases Subject RIV: DG - Athmosphere Sciences, Meteorology

  10. Porovnání současných fyzioterapeutických přístupů v terapii inkontinence v České republice a v zahraničí

    OpenAIRE

    Čížková, Linda

    2017-01-01

    This bachelor thesis focuses on the topic of female urinary incontinence and its physiotherapeutic treatment options. The aim of this thesis is to introduce and compare the current physiotherapy approaches used in the Czech Republic and abroad. This thesis is composed of two parts - theoretical and practical. The theoretical part describes physiotherapy approaches to urinary incontinence in the Czech Republic and in selected foreign countries (Australia, Canada, The United Kingdom, The United...

  11. Časně eneolitické ohrazení ve Vliněvsi. Příspěvek k povaze kontaktu mezi jordanovskou a michelsberskou kulturou v Čechách

    Czech Academy of Sciences Publication Activity Database

    Dobeš, Miroslav; Stránská, Petra; Křivánek, Roman; Limburský, Petr

    2016-01-01

    Roč. 107, prosinec (2016), s. 51-115 ISSN 0031-0506 Institutional support: RVO:67985912 Keywords : Bohemia * Proto-Eneolithic * Jordanów culture * Michelsberg culture * settlement * ditch Subject RIV: AC - Archeology, Anthropology, Ethnology

  12. Úmrtnost související se stresem z horka v České Republice v současném a budoucím klimatu

    Czech Academy of Sciences Publication Activity Database

    Kyselý, Jan; Huth, Radan

    2004-01-01

    Roč. 57, - (2004), s. 113-121 ISSN 0026-1173 R&D Projects: GA ČR GP205/01/D040; GA AV ČR(CZ) KSK3046108 Institutional research plan: CEZ:AV0Z3042911 Keywords : Mortality * Heat stress * Czech Republic Subject RIV: DG - Athmosphere Sciences, Meteorology

  13. Mezníky ve vývoji současného kola mnohostranných liberalizačních jednání

    OpenAIRE

    Vašková, Michaela

    2015-01-01

    The thesis deals with the current round of WTO negotiations. The aim of thesis is to identify the most important milestones of Doha Round development. Particular chapters are devoted to identified milestones, which are the most important ministerial conferences. The thesis describes the process and the outcomes of these conferences and the potential impact of outputs on international trade.

  14. Dilemata ekonomického rozvoje současné české společnosti. K jednomu scénáři možného vývoje

    Czech Academy of Sciences Publication Activity Database

    Benáček, Vladimír

    2010-01-01

    Roč. 8, č. 9 (2010), s. 2-3 ISSN 1214-1720 R&D Projects: GA AV ČR(CZ) IAA700280803 Institutional research plan: CEZ:AV0Z70280505 Keywords : Crisis * politics * reforms Subject RIV: AO - Sociology, Demography http://www.socioweb.cz/upl/editorial/download/182_SOCIOweb_09_2010.pdf

  15. Současné užití barevného fázového kontrastu a polarizační mikroskopie při studiu mikroorganismů

    Czech Academy of Sciences Publication Activity Database

    Žižka, Zdeněk

    2018-01-01

    Roč. 63, č. 4 (2018), s. 122-125 ISSN 0447-6441 R&D Projects: GA MŠk(CZ) LO1509 Institutional support: RVO:61388971 Keywords : color phase contrast * polarization microscopy Subject RIV: EE - Microbiology, Virology OBOR OECD: Microbiology

  16. Local Information Commission of Dampierre-en-Burly, meeting of November 25, 2011. Additional safety assessment. Collection of remarks by the members of the Commission on the report presented by the CNPE to the ASN on 15/9/2011

    International Nuclear Information System (INIS)

    De Ganay, C.

    2011-01-01

    This report first discusses the specifications of the additional nuclear safety assessment. The authors state that this procedure is limited to the robustness of installations and to some climatic events. They formulate several and questions on issues which are not sufficiently or even not at all addressed in these specifications. In a second part, they make remarks on the report made about the Dampierre nuclear power plant. These remarks address the different issues of the assessment: earthquakes, flooding, other extreme natural phenomena, loss of electricity supplies and cooling systems, severe accidents

  17. Vliv zvýšené hladiny dopaminu na chování potkana v časné dospělosti po neonatální aplikaci N-acetylaspartylglutamátu

    Czech Academy of Sciences Publication Activity Database

    Bubeníková, V.; Tejkalová, H.; Klaschka, Jan; Šťastný, F.

    2003-01-01

    Roč. 7, Suppl. 3 (2003), s. 46-49 ISSN 1211-7579 R&D Projects: GA MŠk LN00B122 Keywords : N-acetylaspartylglutamate * schizophrenia * animal model * behavior * GBR 12909 Subject RIV: BA - General Mathematics

  18. Výzkumný projekt "Etika vědy v české republice: Současný stav a jeho historické kořeny"

    Czech Academy of Sciences Publication Activity Database

    Drozenová, Wendy

    2008-01-01

    Roč. 79, 3-4 (2008), s. 327-343 ISSN 1211-7617 R&D Projects: GA AV ČR(CZ) IAA900090803 Institutional research plan: CEZ:AV0Z90090514 Keywords : ethics * science ethics Subject RIV: AA - Philosophy ; Religion

  19. Současná diskuse o sexuální výchově v České republice a stanovisko Římskokatolické církve k této problematice

    OpenAIRE

    Procházková, Petra

    2014-01-01

    This work deals with sex education in the Czech Republic and Roma Catholic's concept of sex education. The first part is focused on concept of sexuality, the definition of sex education, its objectives, principles and methods in relation to the concept of education institutions and selected experts. It also includes a description of the historical context of the development of education. The second part is devoted to the Roman Catholic's view of human sexuality, sexual pedagogy, the role of p...

  20. Islamismus v současném světě a vliv občanské války v Sýrii na jeho šíření

    OpenAIRE

    Havlíčková, Blanka

    2012-01-01

    This diploma thesis analyzes the ideological basis of islamism and deals with the reasons, why this political ideology is becomimg more and more popular in the Middle East and the West. The thesis also analyzes the phenomenon of globalization, in other words the interconnection between islamists in today's world. The last chapter summarizes the facts about the civil war in Syria, where both - islamists from the Middle East, as well as islamists from the West, are involved. The main objective ...

  1. Historie a současná obnova archeologického areálu s bazilikou sv. Vavřince v prostoru NKP Praha-Vyšehrad

    Czech Academy of Sciences Publication Activity Database

    Boháčová, Ivana; Nechvátal, Bořivoj

    2014-01-01

    Roč. 74, č. 1 (2014), s. 3-11 ISSN 1210-5538 Institutional support: RVO:67985912 Keywords : archaeology * Early Middle Ages * in situ presentation * architecture Subject RIV: AC - Archeology, Anthropology, Ethnology

  2. Mediální trh v České republice v kontextu současného hospodářského vývoje

    OpenAIRE

    Machková, Marta

    2011-01-01

    The work focuses on mapping the current situation in the media market in the Czech Republic. It examines in particular the impact of current economic situation in the media organizations. Media behave in the marketplace as commercial enterprises (except public Czech Television and Czech Radio - for comparison are also included in the work), their main objective is to generate profit. Media organizations derive revenue primarily from advertising specified, are therefore dependent on the financ...

  3. In vitro antioxidant activities of the novel pentapeptides Ser-His-Glu-Cys-Asn and Leu-Pro-Phe-Ala-Met and the relationship between activity and peptide secondary structure

    DEFF Research Database (Denmark)

    Yang, Ruiwen; Wang, Jia; Lin, Songyi

    2017-01-01

    ,5-diphenyltetrazolium bromide] assay was used to evaluate cytotoxicity in HepG2 cells. Antioxidant activity was measured using in vitro assays, including the cellular antioxidant activity assay (CAA), 2,2-diphenyl-1-picrylhydrazyl or 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS...... LPFAM (P ABTS inhibition (88.16 ± 0.76%). It had normalized ORAC values of 0.3000 ± 0.0070 µmol GE mg(-1) and 0.0900 ± 0.0020 µmol TE mg(-1) , respectively. The results...

  4. Současný stav nádrží v České republice z hlediska složení rybích obsádek

    Czech Academy of Sciences Publication Activity Database

    Blabolil, Petr; Říha, Milan; Peterka, Jiří; Prchalová, Marie; Vašek, Mojmír; Jůza, Tomáš; Čech, Martin; Draštík, Vladislav; Kratochvíl, Michal; Muška, Milan; Tušer, Michal; Frouzová, Jaroslava; Ricard, Daniel; Šmejkal, Marek; Vejřík, Lukáš; Duras, J.; Matěna, Josef; Borovec, Jakub; Kubečka, Jan

    2014-01-01

    Roč. 64, č. 9 (2014), s. 5-11 ISSN 1211-0760 R&D Projects: GA MŠk(CZ) EE2.3.20.0204 Institutional support: RVO:60077344 Keywords : heavily modified water bodies * species composition * Water Framework Directive Subject RIV: EH - Ecology, Behaviour

  5. Současné podnikatelské prostředí JAR a rozšíření obchodní spolupráce s ČR

    OpenAIRE

    Kuchová, Iveta

    2009-01-01

    This diploma thesis deals with current business environment of the Republic of South Africa which is examined by the PESTEL analysis. It is closely concerned with political, economic, social, technological, environmental and legislative environment of the RSA. This analysis is enriched by practical experience of some Czech entrepreneurs. Simultaneously, the thesis is engaged in business cooperation of this country with the Czech Republic. At the conclusion, possibilities of further extension ...

  6. Kritická analýza současného politického diskursu: Nový jazyk britských labouristů

    Czech Academy of Sciences Publication Activity Database

    Karhanová, Kamila

    2002-01-01

    Roč. 63, č. 2 (2002), s. 111-117 ISSN 0037-7031 R&D Projects: GA MŠk LN00A023 Institutional research plan: CEZ:AV0Z9061902 Keywords : English * analysis * political discourse Subject RIV: AI - Linguistics

  7. Krásné bohyně: současná feminní spiritualita na příkladu ženské tantrické skupiny

    OpenAIRE

    Veselá, Helena

    2010-01-01

    This thesis elaborates the results of the research I carried out wtihin the year 2009 in a paid course of female tantric initiation. The theme of the thesis was inspired by my previous interest in the Goddess movement. As this movement is not yet concretized in the Czech republic, I have chosen participating in a closed women's group which is very close to it due to a specific combination of religion, philosophy and psychology. My goal was to find out what form the women's spirituality has in...

  8. Proměna české kuchyně z dob Magdaleny Dobromily Rettigové po současné trendy zdravého životního stylu

    OpenAIRE

    Málková, Simona

    2018-01-01

    The thesis focuses on the topic of changing eating habits from the time of Magdalena Dobromila Rettigová to contemporary trends of healthy lifestyle. It is a gradual development of eating from the late 18th century to the present. In the thereoretic part there is a discription of the contrast of the Czech writer's life and a present-day lifestyle. We can find a chapter about a life of Magdalena Dobromila Rettigová, background information leading up to, writing the cook book "Cooking at home",...

  9. Současné možnosti a trendy popisu mluvené češtiny (se zaměřením na kolokvializaci a konverzacionalizaci)

    Czech Academy of Sciences Publication Activity Database

    Hoffmannová, Jana

    2008-01-01

    Roč. 77, 1/3 (2008), s. 63-75 ISSN 0037-6736. [Mezinárodní sjezd slavistů /14./. Ochrid, 10.09.2008-16.09.2008] R&D Projects: GA ČR GA405/06/1468; GA ČR GA405/06/1057 Institutional research plan: CEZ:AV0Z90610518 Keywords : spoken Czech * colloquialization * converzationalization * intimate style * genre * diglossia * pragmatics Subject RIV: AI - Linguistics

  10. Možnosti využití současných poznatků v oblasti zrcadlových neuronů v psychologické praxi pro trénink empatie

    OpenAIRE

    Poupětová, Veronika

    2017-01-01

    Mirror neurons are very specific nerve cells that are both motor and sensory in nature. Mirror neurons are a relatively new discovery first identified by a team of neurophysiologists at the University of Parma. These neurons were first observed in primates, and then later in humans in several regions of the cerebral cortex. A large amount of research on mirror neurons have shown that they play an important role in imitation, language acquisition and empathy. The theoretical part of this work ...

  11. Internet jako prostor pro sociální inovace? Analýza prostředí digitálních podob současného českého tisku

    OpenAIRE

    Soukupová, Lenka

    2013-01-01

    Taking into account the theoretical approaches of Joseph Alois Schumpeter and Clayton Christensen to innovation, this case study is mapping the current Czech environment of written online media. Traditional media houses are dealing with the crisis of finances and information as generally described by Christensen, which means they are in real need for innovation yet they are not always successful. Other new incentives also appear on the market. Similarly to the situation abroad, the NGO sector...

  12. 135-Day Interventions of Yam Dioscorin and the Dipeptide Asn-Trp (NW) To Reduce Weight Gains and Improve Impaired Glucose Tolerances in High-Fat Diet-Induced C57BL/6 Mice.

    Science.gov (United States)

    Wu, Guang-Cheng; Lin, Shyr-Yi; Liang, Hong-Jen; Hou, Wen-Chi

    2018-01-24

    The C57BL/6J mice were fed a 135-day normal diet or a high-fat diet (HFD) without, or concurrent with, a single yam dioscorin (80 mg/kg) or dipeptide NW (40 mg/kg) intervention every day. The final body weights (g) of mice were 26.1 ± 1.4, 34.97 ± 2.1, 31.75 ± 2.6, and 31.66 ± 3.1, respectively, for normal diet-fed, HFD-fed, dioscorin-intervened, and NW-intervened group. The mice in both intervened groups showed similar less weight gains and had significant differences (P index of mice with dioscorin interventions showed significantly lower contents in total cholesterol and low-density lipoprotein, and NW interventions showed significantly lower total triglyceride contents compared to those of the HFD group (P glucose levels by oral glucose tolerance tests and both showed significant differences (P glucose tolerance controls, which require further clinical trial investigations.

  13. Časně eneolitické měděné sekery z Buchlovic a Uherského Hradiště, katastrálního území Sady

    Czech Academy of Sciences Publication Activity Database

    Menoušková, D.; Fikrle, Marek

    LV, č. 2014 (2014), s. 181-192 ISSN 0583-5569 Institutional support: RVO:61389005 Keywords : Eneolithic Copper Axes * material analysis * Moravian history Subject RIV: AC - Archeology, Anthropology, Ethnology

  14. Domov jako konceptuální rámec, téma i výzkumný terén: vývoj i současná podoba studií domova

    Czech Academy of Sciences Publication Activity Database

    Gibas, Petr

    2017-01-01

    Roč. 53, č. 2 (2017), s. 241-268 ISSN 0038-0288 R&D Projects: GA ČR(CZ) GAP404/12/1446 Institutional support: RVO:68378025 Keywords : home * house and home * phenomenology of home Subject RIV: AO - Sociology, Demography OBOR OECD: Sociology Impact factor: 0.143, year: 2016 http://sreview.soc.cas.cz/cs/issue/188-sociologicky-casopis-czech-sociological-review-2-2017/3706? culture =en

  15. Současné tendence ve výzkumu péče: Zpráva z mezinárodní konference Critical Care

    Czech Academy of Sciences Publication Activity Database

    Uhde, Zuzana

    2012-01-01

    Roč. 13, č. 2 (2012), s. 113-115 ISSN 1213-0028. [Critical Care: Advancing an Ethics of Care in Theory and Practice. Brighton, 13.09.2012-14.09.2012] R&D Projects: GA ČR GAP404/10/0021 Institutional support: RVO:68378025 Keywords : care * gender * conference Subject RIV: AO - Sociology, Demography

  16. Historie a současný stav urychlovačů částic v českých zemích. I. Lineární urychlovače

    Czech Academy of Sciences Publication Activity Database

    Janovský, I.; Šimáně, Čestmír

    2012-01-01

    Roč. 62, č. 3 (2012), s. 157-168 ISSN 0009-0700 Institutional support: RVO:61389005 Keywords : accelerator history * linear accelerator s Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders

  17. Molecular cloning of a preprohormone from Hydra magnipapillata containing multiple copies of Hydra-L Wamide (Leu-Trp-NH2) neuropeptides: evidence for processing at Ser and Asn residues

    DEFF Research Database (Denmark)

    Leviev, I; Williamson, M; Grimmelikhuijzen, C J

    1997-01-01

    The simple, freshwater polyp Hydra is often used as a model to study development in cnidarians. Recently, a neuropeptide, metamorphosis in a hydroid planula larva to become a polyp. Here, we have cloned a preprohormone...... from Hydra magnipapillata containing 11 (eight different) immature neuropeptide sequences that are structurally related to the metamorphosis-inducing neuropeptide from sea anermones. During the final phase of our cloning experiments, another research team independently isolated and sequenced five...... most frequent one being Gly-Pro-Pro-Pro-Gly-Leu-Trp-NH2; Hydra-LWamide l; three copies). Based on their structural similarities with the metamorphosis-inducing neuropeptide from sea anemones, the mature peptides derived from the Hydra-LWamide preprohormone are potential candidates for being...

  18. Íránská islámská revoluce a její reflexe v současném Íránu

    OpenAIRE

    Běhalová, Eliška

    2014-01-01

    Bachelor thesis focuses on Iranian Islamic Revolution of 1979 which fundamentally influenced relations and development in the Middle East. The aim is to detect the presence of the principles of the revolution in contemporary Iran. The first two chapters characterize the revolution in general, the remaining three chapters focus on the process of revolution, achieved changes and its reflextion in Iran in the period from 2000 to 2016.

  19. Sociálně založené nerovnosti ve zdraví: současný vývoj ve světě a stav u nás

    Czech Academy of Sciences Publication Activity Database

    Šolcová, Iva; Kebza, V.

    2002-01-01

    Roč. 46, č. 3 (2002), s. 219-224 ISSN 0009-062X Institutional research plan: CEZ:AV0Z7025918 Keywords : inequalities in health * SES * self-reported health Subject RIV: AN - Psychology Impact factor: 0.190, year: 2002

  20. Současné trendy v párování materiálů artikulačních komponent kloubních náhrad

    Czech Academy of Sciences Publication Activity Database

    Fulín, P.; Pokorný, D.; Šlouf, Miroslav; Jahoda, D.; Sosna, A.

    -, č. 2 /15/ (2011), s. 24-27 ISSN 1803-3679 R&D Projects: GA TA ČR TA01011406 Institutional research plan: CEZ:AV0Z40500505 Keywords : total joint replacement * hard-on-hard bearings * hard-on-soft bearings Subject RIV: CD - Macromolecular Chemistry http://www. medicina aumeni.cz/index.php?action=27

  1. Současné postupy v diagnostice, léčbě a prevenci infekce vyvolané methicilin rezistentními kmeny bakterie Staphylococcus aureus (MRSA)

    OpenAIRE

    Korejsová, Erika

    2013-01-01

    Candidate: Erika Korejsová Supervisor: Mgr. Klára Konečná, Ph.D. Consultant: MUDr. Marie Smolíková Title: Current methods in diagnostics, treatment and prevention of infection caused by methicilin resistant Staphylococcus aureus (MRSA) Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study field: Medical Bioanalytics Staphylococcus aureus is a bacterium that is found in the nasal mucosa of healthy people (20-40%)...

  2. Ke zpracování názvů rostlin v Akademickém slovníku současné češtiny

    Czech Academy of Sciences Publication Activity Database

    Nová, Jana; Pernicová, Helena

    2016-01-01

    Roč. 99, č. 4 (2016), s. 207-212 ISSN 0027-8203 R&D Projects: GA MK DF13P01OVV011 Keywords : monolingual dictionary * lexicography * botany * genus * species * genus proximum * polysemy * family Subject RIV: AI - Linguistics

  3. Realismus v české literatuře 19. století. Pohledy současníků, historické interpretace a badatelské perspektivy

    Czech Academy of Sciences Publication Activity Database

    Hrdina, Martin

    2014-01-01

    Roč. 62, č. 3 (2014), s. 372-394 ISSN 0009-0468 R&D Projects: GA ČR GPP406/12/P839 Institutional support: RVO:68378068 Keywords : literary realism * Czech literature of the 19th century * methodology of literary history Subject RIV: AJ - Letters, Mass-media, Audiovision

  4. Uranium mineralisation in Barapani formation of Mawbeh Area, East Khasi Hills District, Meghalaya

    International Nuclear Information System (INIS)

    Gupta, C.S.; Nagendra Kumar, M.; Majumdar, Amit; Umamaheshwar, K.

    2008-01-01

    Proterozoic Shillong Basin of Meghalaya comprises metapelites of Paleoproterozoic Tyrsad and arenaceous siliciclastics of Mesoproterozoic Barapani formations. Two major igneous activities, in the form of basic dykes/sills and younger granites of Neoproterozoic age, intruding Proterozoic sediments, are reported from Shillong Basin. Significant uranium mineralisation, with values up to 0.1% U 3 O 8 , associated with NE-SW trending shear zone in Barapani Formation is discovered at Mawbeh area, Pynursla Plateau. The mineralised Barapani has undergone hydrothermal alterations in the form of sericitisation, chloritisation, illitisation and kaolinisation. Petrographic studies reveal that the host rocks are ortho-quartzite, subfeldspathic arenites, quartz wacke, sericite phyllite, quartz-sericite-chlorite rock and quartz wacke. X-ray diffraction (XRD) studies of radioactive Barapani quartzite revealed the presence of uraninite. (author)

  5. 2016 Quantum Science Gordon Research Conference

    Science.gov (United States)

    2018-01-10

    Poster Presenter Registered Barik , Sabyasachi IREAP Attendee Registered Beck, Mark Whitman College Poster Presenter Registered Béguin, Jean...Innsbruck Speaker Registered Bollinger, John J National Institute of Standards and Technology Speaker Registered Bondar, Denys I Princeton...Tatjana AFOSR Attendee Registered Dauphinais, Guillaume Université de Sherbrooke Poster Presenter Registered Davis, Emily J Stanford University

  6. Voyage: espace et poésie dans un calligramme d'Apollinaire

    Directory of Open Access Journals (Sweden)

    Laurent GRISON

    1998-06-01

    Full Text Available L'œuvre de Guillaume Apollinaire offre une approche spécifique de la poésie et de l'espace. L'étude d'un calligramme, Voyage, nous permet de souligner à quel point cet auteur mêle travail poétique et réflexion sur la spatialité.

  7. Engineering activities on New Built Projects

    International Nuclear Information System (INIS)

    Puravet, Guillaume

    2014-01-01

    Guillaume Puravet, Director of Operations at Assystem, described the engineering activities related to nuclear new builds. He presented the various competencies that Assystem can provide to its customers all along the construction phase of a new nuclear project from the conceptual design to its commissioning

  8. Reference: 656 [Arabidopsis Phenome Database[Archive

    Lifescience Database Archive (English)

    Full Text Available al Gakière Bertrand|Hoeberichts Frank A|Issakidis-Bourguet Emmanuelle|Miginiac-Maslow Myroslawa|Noctor Graham|Queval Guillaume|Van Breusegem Frank|Vanacker Hélène|Vandorpe Michaël

  9. Narva-teemaline magistritöö Pariisis / Merike Trubert

    Index Scriptorium Estoniae

    Trubert, Merike

    2002-01-01

    27. XI kaitsesid prantsuse arhitektuuriüliõpilased David Trubert ja Guillaume Blanchard (1999-2001 vahetusüliõpilased EKAs) Pariisi arhitektuurikoolis (Ecole d'Architecture de Paris Val-de-Seine) Narva linnaplaneeringut käsitleva magistritöö "Narva. Urban planning for social approach". Kaitsmiskomisjoni juhataja Mart Kalm

  10. Effects of Apis mellifera adansonii, L. 1758 (Apidae: Hymenoptera ...

    African Journals Online (AJOL)

    Effects of Apis mellifera adansonii, L. 1758 (Apidae: Hymenoptera) pollination on yields of Cucumeropsis mannii (Naudin) in Kisangani, Democratic Republic of Congo. Boniface Posho Ndola, Yves Brostaux, Guillaume Le Goff, Marie-Lucie Susini, Eric Haubruge, Frederic Francis, Bach Kim Nguyen ...

  11. Échinodermes, peintes d’après nature par les soins de Kuhl, van Hasselt et Sal. Müller, membres de la Commission pour l’exploration physique des possessions d’outre-mer des Pays-Bas, publiées d’après les cartons du Musée Royal d’histoire naturelle à Leide

    NARCIS (Netherlands)

    Herklots, J.A.

    1869-01-01

    La publication des planches qui composent le présent recueil me fournit l’occasion de faire connaître quelques uns des fruits de la libéralité, avec laquelle feu notre auguste Roi GUILLAUME I a daigné subvenir aux sciences, tout en faisant faire l’exploration de nos possessions d’outre-mer. Des

  12. Data of evolutionary structure change: 1ANCA-2HNTE [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1ANCA-2HNTE 1ANC 2HNT A E IVGGYTCQENSVPYQVSLNSGYHFCGGSLINDQWVVSAA...> 0 1ANC A 1ANC...1 1.00 16.75 C e-map> ILE ASP ASN ASN LEU THR LYS ARG ASP PHE ASN CA 5.65 3.82 ASP CA 3.82 ILE CA ...21.33 C e-map> ILE ASP ARG ASP LEU ASN

  13. The formal notices of the French authority of nuclear safety; Les mises en demeure de l'autorite de surete nucleaire

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    The French authority of nuclear safety (ASN) publishes on its web site (http://www.asn.gouv.fr) the formal notices and official statements addressed by the ASN to the concerned responsible persons (operators of nuclear facilities, directors of companies etc..) when anomalies requiring a corrective action have been noticed during on-site or off-site safety inspections. This document brings together the formal notices addressed by the ASN since June 2000 and up to April 2002. (J.S.)

  14. Characterization of sialylated and fucosylated glycopeptides of beta2-glycoprotein I by a combination of HILIC LC and MALDI MS/MS

    DEFF Research Database (Denmark)

    Kondo, Akira; Thaysen-Andersen, Morten; Hjernø, Karin

    2010-01-01

    were characterized using MALDI quadrupole TOF MS/MS. A total of 23 glycan structures, including sialylated bi- and tri-antennary complex type glycans, were characterized at three N-glycosylation sites, namely Asn-143, Asn-174 and Asn-234, of beta2-GPI. Further exploration of the complementary nature...

  15. Glycosylation of the N-terminal potential N-glycosylation sites in the human α1,3-fucosyltransferase V and -VI (hFucTV and -VI)

    DEFF Research Database (Denmark)

    Christensen, Lise Lotte; Bross, Peter Gerd; Ørntoft, Torben Falck

    2000-01-01

    Human alpha1,3-fucosyltransferase V and -VI (hFucTV and -VI) each contain four potential N-glycosylation sites (hFucTV: Asn60, Asn105, Asn167 and Asn198 and hFucTVI: Asn46, Asn91, Asn153 and Asn184). Glycosylation of the two N-terminal potential N-glycosylation sites (hFucTV: Asn60, Asn105 and h......FucTVI: Asn46 and Asn91) have never been studied in detail. In the present study, we have analysed the glycosylation of these potential N-glycosylation sites. Initially, we compared the molecular mass of hFucTV and -VI expressed in COS-7 cells treated with tunicamycin with the mass of the proteins...... in untreated cells. The difference in molecular mass between the proteins in treated and untreated cells corresponded to the presence of at least three N-linked glycans. We then made a series of mutants, in which the asparagine residues in the N-terminal potential N-glycosylation sites were replaced...

  16. Relationship Between Genotype Variants Follicle-stimulating Hormone Receptor Gene Polymorphisms (FSHR) and Morphology of Oocytes Prior to ICSI Procedures

    Science.gov (United States)

    Gashi, Zafer; Elezaj, Shkelzen; Zeqiraj, Afrim; Grabanica, Driton; Shabani, Isak; Gruda, Bujar; Gashi, Fitore

    2016-01-01

    Introduction: This study investigated association of Asn680Ser FSHR polymorphism with the ovarian response in 104 women of Albanian ethnic population enrolled in ICSI program. The reason of infertility in all cases has been identified as male factor. Methods: Analysis of the Asn680Ser polymorphism was performed using TaqMan® SNP Genotyping Assay. Clinical and endocrinologic parameters were analyzed based on the genotype, age, BMI, oocyte yield, number of transferred embryos and pregnancy rate. Results: The frequencies of the Asn680 Ser genotype variants were as follows: Asn/Asn 22.1%, Asn/Ser 47.1%, and Ser/Ser 30.8%, respectively. BMI was significantly higher in the Ser/Ser group as compared to those from the Asn/Ser or the Asn/Asn group (p= 0.0010). The genotype variants Ser/Ser indicates a higher rate of oocyte retrieval (25.9%) in the immature form, metaphase I (MI) as opposed to the other two groups (Asn/Asn 23.7 % vs. Asn/Ser 21.9%), which was statistically significant (p = 0.3020). Conclusions: FSH receptor polymorphism is associated with different ovarian response to controlled ovarian stimulation (COS), but is not an important factor in increasing the degree of pregnancy. Polymorphisms of the FSH receptor is associated with normal morphology and genetic maturation (metaphase II) oocytes in dependence of genotypic variation polymorphisms. PMID:27994298

  17. Local Information Commission of Dampierre-en-Burly, meeting of November 25, 2011. Additional safety assessment. Collection of remarks by the members of the Commission on the report presented by the CNPE to the ASN on 15/9/2011; Commission locale d'information de Dampierre-En-Burly, Reunion du 25 novembre 2011. Evaluation complementaire de surete. Recueil d'observations de membres de la Commission sur le rapport presente par le CNPE a l'ASN le 15/9/2011

    Energy Technology Data Exchange (ETDEWEB)

    De Ganay, C.

    2011-11-29

    This report first discusses the specifications of the additional nuclear safety assessment. The authors state that this procedure is limited to the robustness of installations and to some climatic events. They formulate several and questions on issues which are not sufficiently or even not at all addressed in these specifications. In a second part, they make remarks on the report made about the Dampierre nuclear power plant. These remarks address the different issues of the assessment: earthquakes, flooding, other extreme natural phenomena, loss of electricity supplies and cooling systems, severe accidents

  18. Interaction between viologen-phosphorus dendrimers and α-synuclein

    International Nuclear Information System (INIS)

    Milowska, Katarzyna; Grochowina, Justyna; Katir, Nadia; El Kadib, Abdelkrim; Majoral, Jean-Pierre; Bryszewska, Maria; Gabryelak, Teresa

    2013-01-01

    In this study the interaction between viologen-phosphorus dendrimers and α-synuclein (ASN) was examined. Polycationic viologen-phosphorus dendrimers (two positive charges per viologen unit) are novel compounds with relatively unknown properties. The influence of these viologen dendrimers on ASN was tested using fluorimetric and circular dichroism methods. ASN contains four tyrosine residues; therefore, the influence of dendrimers on protein molecular conformation by measuring the changes in the ASN fluorescence in the presence of dendrimers was evaluated. The interaction of dendrimers with free L-tyrosine was also monitored. Results show that viologen-phosphorus dendrimers interact with ASN; they quenched the fluorescence of ASN as well as free tyrosine by dynamic and static ways. However, the quenching was not accompanied by modifications in the ASN secondary structure. - Highlights: ► Interaction between viologen-phosphorus dendrimers and α-synuclein (ASN) was investigated. ► Viologen-phosphorus dendrimers can quench the fluorescence of tyrosine in ASN. ► Dendrimers caused red-shift in maximum of fluorescence. ► Viologen-phosphorus dendrimers did not change the secondary structure of ASN.

  19. Interaction between viologen-phosphorus dendrimers and {alpha}-synuclein

    Energy Technology Data Exchange (ETDEWEB)

    Milowska, Katarzyna, E-mail: milowska@biol.uni.lodz.pl [Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz (Poland); Grochowina, Justyna [Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz (Poland); Katir, Nadia [Laboratoire de Chimie de Coordination CNRS, 205 route de Narbonne, 31077 Toulouse (France); El Kadib, Abdelkrim [Institute of Nanomaterials and Nanotechnology (INANOTECH)-MAScIR (Moroccan Foundation for Advanced Science, Innovation and Research), ENSET, Avenue de I' Armee Royale, Madinat El Irfane, 10100 Rabat (Morocco); Majoral, Jean-Pierre [Laboratoire de Chimie de Coordination CNRS, 205 route de Narbonne, 31077 Toulouse (France); Bryszewska, Maria; Gabryelak, Teresa [Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz (Poland)

    2013-02-15

    In this study the interaction between viologen-phosphorus dendrimers and {alpha}-synuclein (ASN) was examined. Polycationic viologen-phosphorus dendrimers (two positive charges per viologen unit) are novel compounds with relatively unknown properties. The influence of these viologen dendrimers on ASN was tested using fluorimetric and circular dichroism methods. ASN contains four tyrosine residues; therefore, the influence of dendrimers on protein molecular conformation by measuring the changes in the ASN fluorescence in the presence of dendrimers was evaluated. The interaction of dendrimers with free L-tyrosine was also monitored. Results show that viologen-phosphorus dendrimers interact with ASN; they quenched the fluorescence of ASN as well as free tyrosine by dynamic and static ways. However, the quenching was not accompanied by modifications in the ASN secondary structure. - Highlights: Black-Right-Pointing-Pointer Interaction between viologen-phosphorus dendrimers and {alpha}-synuclein (ASN) was investigated. Black-Right-Pointing-Pointer Viologen-phosphorus dendrimers can quench the fluorescence of tyrosine in ASN. Black-Right-Pointing-Pointer Dendrimers caused red-shift in maximum of fluorescence. Black-Right-Pointing-Pointer Viologen-phosphorus dendrimers did not change the secondary structure of ASN.

  20. Members of the Forum Engelberg visit CERN

    CERN Multimedia

    Patrice Loïez

    2002-01-01

    The Forum Engelberg is an annual interdisciplinary conference held in Engelberg, Switzerland intended to act as an international platform for debate and exchange of views on key issues affecting scientific research, technology, economics and philosophy. Its President is Hubert Curien - former French Minister of Research and Space Research, and President of the CERN Council from 1994 to 1996. He is seen here visiting building SM18, which houses String 2: the test facility for LHC systems. Photo 01: (left to right) Mme Jaconi, Prof. Roger Berthouzoz, M. Guillaume Pictet, M. Claude Bagnoud, R. Saban and Hubert Curien. Photo 02: (left to right) R. Saban, M. Jean-Claude Rochat, Hubert Curien and M. Guillaume Pictet. Photo 03: (left to right) R. Saban, M. Jean-Claude Rochat and Hubert Curien.

  1. Pop / Mart Juur

    Index Scriptorium Estoniae

    Juur, Mart, 1964-

    2002-01-01

    Heliplaatidest: Holly Valance "Footprints", Peven Everett "Studio Confessions", filmimuusika "The Guru", Onyx "Bacdafucup Part II", The Wallflowers "Red Letter Days", Chris Rea "Stony Road", Public Enemy "Revolverolution", Roxette "The Ballad Hits", Jephte Guillaume & The Tet Kale Orkestra "Voyage Of Dreams", Dictaphone "m.=addiction", Spaceheads "Low pressure", erinevad esitajad "The Best Of Bond James Bond", Jeff Mills "At First Sight", Tracy Chapman "Let it Rain"

  2. Loomingu eluandev jõud / Siim Angerpikk

    Index Scriptorium Estoniae

    Angerpikk, Siim

    2015-01-01

    Maailmafilmi festivali raames linastuvatest filmidset "Gualdino üheksas elu" (rež. Filipe Araújo), Portugal-Prantsusmaa; "C-minoori eripärad (rež. Ollie Lucks, Max Bellamy), Uus-Meremaa; "Salto Mortale" (rež. Guillaume Kozakiewiez), Prantsusmaa-Šveits;"Tantsud Mariaga" (rež. Ivan Gergolet), Itaalia-Argentiina-Sloveenia. Filme ühendab loomeinimeste püüd üleatda takistusi ja piire oma igapäevatöös.

  3. Geografia, educação libertária e escola pública: um programa de emancipação através do saber

    OpenAIRE

    Ferretti, Federico

    2014-01-01

    International audience; This paper deals with the role played by the anarchist geographers Elisée Reclus and Peter Kropotkin in the foundation of the movement of libertarian schools, popular universities and university extensions in Europe between the 19th and the 20th centuries, which is still not very known in the historiography on libertarian pedagogy. At the same time, we stress the role of anarchist scientists like James Guillaume, close to Reclus and Kropotkin, in building the French sy...

  4. At the Fulcrum of Air Force Identity: Balancing the Internal and External Pressures of Image and Culture

    Science.gov (United States)

    2010-06-01

    and Commitment and Their Relationships to Turnover Intention: Does Organizational Heirarchy Matter?‖ Journal of Organizational Behaviour 27, no. 5...For the practical consumer , John Balmer, Guillaume Soenen, and Stephen Greyser acted on this premise to produce the trademarked AC2ID test, focused...television advertising, following the trail the Army so famously blazed with its ―Be All You Can Be‖ jingle in 1981.50 Use of the medium of television

  5. French Intensive Care Society, International congress ? R?animation 2016

    OpenAIRE

    Jaillette, Emmanuelle; Girault, Christophe; Brunin, Guillaume; Zerimech, Farid; Chiche, Arnaud; Broucqsault-Dedrie, C?line; Fayolle, Cyril; Minacori, Franck; Alves, Isabelle; Barrailler, Stephanie; Robriquet, Laurent; Delaporte, Emmanuel; Thellier, Damien; Delcourte, Claire; Duhamel, Alain

    2016-01-01

    Table of contents PHYSICIANS ABSTRACTS O1 Impact of tracheal cuff shape on microaspiration of gastric contents in intubated critically ill patients: a multicenter randomized controlled study (BEST CUFF) Emmanuelle Jaillette, Christophe Girault, Guillaume Brunin, Farid Zerimech, Arnaud Chiche, C?line Broucqsault-Dedrie, Cyril Fayolle, Franck Minacori, Isabelle Alves, Stephanie Barrailler, Laurent Robriquet, Fabienne Tamion, Emmanuel Delaporte, Damien Thellier, Claire Delcourte, Alain Duhamel, ...

  6. Browse Author Index

    African Journals Online (AJOL)

    Items 1 - 50 of 424 ... Ballet, J · Banks, M · Barrios, Luis Garcia · Belle, EM · Belle, EMS · Bertrand, A · Besnard, Guillaume · Birkinshaw, C · Birkinshaw, CB · Birkinshaw, Christopher R. Blanthorn, Tara S. Bollen, A · Borgerson, C · Botero, Natalia · Brenier, A · Brimont, Laura · Buckley, GA · Burney, D · Burney, DA · Bussmann ...

  7. Reformulating Immigration Policy in Post-Apartheid South Africa : From the Aliens Control Act of 1991 to the Immigration Act of 2002

    OpenAIRE

    Wa Kabwe-Segatti , Aurelia

    2006-01-01

    Volume entitled: Ten Years of Democratic South Africa. Transition Accomplished? edited by Aurelia Wa Kabwe-Segatti, Nicolas Péjout and Philippe Guillaume; While socio-political and institutional transformations have been extremely rapid over the past ten years, the reform of the South African immigration policy and legislation has been delayed for almost a decade. Looking back at the system in place when the ANC took office in 1994, the author describes the successive management of migration ...

  8. [Charles-Henri Fialon (1846-1933). Creator of the historical museum of the faculty of pharmacy of Paris].

    Science.gov (United States)

    Bzoura, Elie; Flahaut, Jean

    2004-01-01

    Charles-Henri Fialon stopped his pharmaceutical activities in 1892 and he devoted his time to artistic and historic works. He achieved an important collection of pharmaceutical pots and objects which he gave to the school of Pharmacy of Paris. These gifts were collected in a room named "Musée Fialon ". This museum was enlarged twice and presently is in the "Guillaume Valette" gallery. His content is described in this paper.

  9. [Charles-Henri Fialon (1846-1933), creator of the Museum of the Faculty of Pharmacy of Paris. His family and professional life].

    Science.gov (United States)

    Flahaut, Jean

    2004-01-01

    Henri Fialon was pharmacist in 1871, and worked in the family apothecary, at Rueil near Paris. At 46 years, he stopped his pharmaceutical activity, and livre quietly, being specially interested by the ancient pharmaceutical pots. He realized a very important collection, which he gave to the French Society of Pharmaceutical History. A museum was installed at the Faculty of Pharmacy of Paris which is presently in the Guillaume Valette Gallery and contains more than 500 pieces.

  10. Synergistic Cytotoxic Effect of L-Asparaginase Combined with Decitabine as a Demethylating Agent in Pediatric T-ALL, with Specific Epigenetic Signature

    Directory of Open Access Journals (Sweden)

    Salvatore Serravalle

    2016-01-01

    Full Text Available T-Acute Lymphoblastic Leukemia (T-ALL remains a subgroup of pediatric ALL, with a lower response to standard chemotherapy. Some recent studies established the fundamental role of epigenetic aberrations such as DNA hypermethylation, to influence patients’ outcome and response to chemotherapy. Moreover, L-asparaginase is an important chemotherapeutic agent for treatment of ALL and resistance to this drug has been linked to ASNS expression, which can be silenced through methylation. Therefore, we tested whether the sensitivity of T-ALL cell lines towards L-asparaginase is correlated to the epigenetic status of ASNS gene and whether the sensitivity can be modified by concurrent demethylating treatment. Hence we treated different T-ALL cell lines with L-asparaginase and correlated different responses to the treatment with ASNS expression. Then we demonstrated that the ASNS expression was dependent on the methylation status of the promoter. Finally we showed that, despite the demethylating effect on the ASNS gene expression, the combined treatment with the demethylating agent Decitabine could synergistically improve the L-asparaginase sensitivity in those T-ALL cell lines characterized by hypermethylation of the ASNS gene. In conclusion, this preclinical study identified an unexpected synergistic activity of L-asparaginase and Decitabine in the subgroup of T-ALL with low ASNS expression due to hypermethylation of the ASNS promoter, while it did not restore sensitivity in the resistant cell lines characterized by higher ASNS expression.

  11. The control of base nuclear facilities (I.N.B.); Le controle des installations nucleaires de base (INB)

    Energy Technology Data Exchange (ETDEWEB)

    Anon

    2009-02-15

    The Authority of Nuclear Safety ( A.S.N). presents in this column the current events of the control of the nuclear basic installations during august, september, october 2008, classified by nuclear site. This information is also available in real-time on the A.S.N. web site, www.asn.fr, in the column 'news'. We can consult all the notices of significant incident published as well as the following letters of inspection, the notices of information about the reactors shutdown, press releases and the A.S.N. information notes. (N.C.)

  12. The control of base nuclear installations; Le controle des installations nucleaires de base (INB)

    Energy Technology Data Exchange (ETDEWEB)

    Anon

    2009-04-15

    The Authority of Nuclear Safety ( A.S.N). presents in this column the current events of the control of the nuclear base installations during november, december 2008 and january 2009, classified by nuclear site. This information is also available in real-time on the A.S.N. web site, www.asn.fr, in the column 'news'. We can consult all the notices of significant incident published as well as the following letters of inspection, the notices of information about the reactors shutdown, press releases and the A.S.N. information notes. (N.C.)

  13. Přehřátý lidský kapitál. Současné pohrdání vědou a výzkumem nás bude pěkně mrzet

    Czech Academy of Sciences Publication Activity Database

    Jurajda, Štěpán

    2004-01-01

    Roč. 15, č. 37 (2004), s. 9-9 ISSN 0862-6545 R&D Projects: GA AV ČR KSK9058117 Institutional research plan: CEZ:AV0Z7085904 Keywords : human capital * science and research * Czech Republic Subject RIV: AH - Economics

  14. Muži ve válce, válka v mužích. Maskulinity a světové války 20. století v současné kulturní historiografii

    Czech Academy of Sciences Publication Activity Database

    Kučera, Rudolf

    2011-01-01

    Roč. 19, č. 4 (2011), s. 549-562 ISSN 1210-7050 R&D Projects: GA ČR GPP410/10/P316 Institutional research plan: CEZ:AV0Z70900502 Keywords : World War I * World War II * masculinity, history Subject RIV: AB - History

  15. Napodobenina červenofigurové keramiky z časně laténského sídliště v Chržíně s doklady kovolitectví a zpracování jantaru

    Czech Academy of Sciences Publication Activity Database

    Chytráček, Miloslav

    2007-01-01

    Roč. 59, č. 3 (2007), s. 461-516 ISSN 0323-1267 R&D Projects: GA AV ČR IAA8002204 Institutional research plan: CEZ:AV0Z80020508 Keywords : Bohemia * early La Tène period * imitations * red -figure decoration * metallurgy * amber * long-distance roads * imports * Attic ceramic Subject RIV: AC - Archeology, Anthropology, Ethnology

  16. Pojmenování barev a jejich odstínů v Akademickém slovníku současné češtiny : Names of Colours and Colour Shades in the Academic Dictionary of Contemporary Czech

    Directory of Open Access Journals (Sweden)

    Lišková, Michaela

    2015-10-01

    Full Text Available The paper deals with processing the entries describing colours in the Academic Dictionary of Contemporary Czech (ASSČ, the priority being the explanation of basic meanings of primary colours and lexicalised colour shades. The definitions ought to include all semantic features, be clear and should reflect the present world and the experience of the user. Special attention is given to the explanations of adverbs and compound adjectives. The paper also follows the decision-making process leading to the list of entries within a given category, based on the frequency of the lemma in the SYN corpus, and discusses the problem of appropriate exemplification.

  17. Mezinárodní systém fotografií pro výzkum emocí: jeden ze současných přístupů ke zkoumání emočních stavů

    Czech Academy of Sciences Publication Activity Database

    Czekóová, Kristína; Urbánek, Tomáš

    2010-01-01

    Roč. 54, č. 3 (2010), s. 277-289 ISSN 0009-062X R&D Projects: GA ČR GA406/09/2089 Institutional research plan: CEZ:AV0Z70250504 Keywords : emotion research * IAPS * psychosemantics Subject RIV: AN - Psychology Impact factor: 0.230, year: 2010

  18. Archeologický výzkum novověku a moderní doby v České republice. Několik poznámek k historii, současnému stavu a perspektivám

    Directory of Open Access Journals (Sweden)

    Václav Matoušek

    2017-10-01

    Full Text Available The beginnings of archaeological interest in studying the modern era can be dated to the early 20th century. Initially, isolated initiatives and achievements were based on research of archaeologists (especially specialists in the Middle Ages, ethnographers, and even exceptionally historians. In 1980s the interest in learning about our history of modern times through archeology significantly strengthened so that it is quite common in the Czech Republic today. However, the “post-medieval” concept still prevails, i. e. an interest in the monitoring of medieval phenomena, traditional or cultural artifacts, or the medieval technologies is still preferred, and only in the new dimensions of time – in modern times. The issue of the new phenomena, e. g. industrial society and culture, or the study of present is in the Czech Republic still at the edge of archaeological interest.

  19. Tak v současných mediálních mluvených rozhovorech: ty zákony co se vztahujou teďkon na nás soukromníky (2. část)

    Czech Academy of Sciences Publication Activity Database

    Jílková, Lucie

    -, č. 15 (2017), s. 31-46 ISSN 1804-137X R&D Projects: GA ČR GA15-01116S Institutional support: RVO:68378092 Keywords : word tak * DIALOG corpus * mass media dialogue * spoken language * meanings and functions of the Czech word tak Subject RIV: AI - Linguistics OBOR OECD: Linguistics

  20. Kvalita života dětí po léčbě nádorového onemocnění: současné poznatky a směry výzkumu

    Czech Academy of Sciences Publication Activity Database

    Blatný, Marek; Kepák, T.; Vlčková, I.; Pilát, M.; Jelínek, Martin; Slezáčková, Alena; Navrátilová, P.; Kárová, Š.; Štěrba, J.

    2007-01-01

    Roč. 42, č. 4 (2007), s. 291-306 ISSN 0555-5574 R&D Projects: GA ČR GA406/07/1384 Institutional research plan: CEZ:AV0Z70250504 Keywords : tumor diseases * late effects of therapy * quality of life Subject RIV: AN - Psychology

  1. Současný krizový management středních odborných škol - vliv poklesu stavu žáků na středních odborných školách a odborných učilištích v Praze

    OpenAIRE

    Tuška, Pavel

    2012-01-01

    TITLE: The current risk management at secondary vocational schools - effect of the decline of students in secondary vocational schools and vocational schools in Prague. AUTHOR: Bc. Pavel Tuška DEPARTMENT: The Centre of the educational management SUPERVISOR: RNDr. Jana Marková ABSTRACT: The prosperity of every secondary vocational school and vocational school is primarily based on the demand for their service which also depends on the behaviour of the school in times of crisis. The rapid demog...

  2. Stravovací návyky a pohybový režim současných českých dětí a adolescentů ve věku 11 - 13 let

    OpenAIRE

    Maratová, Klára

    2015-01-01

    1 ABSTRACT The prevalence of overweight and obesity is constantly growing all over the world. Not only adults, but also children and adolescents are affected by this increase. The development of overweight or obesity in childhood represents a rather significant health risk that extends to adulthood. Both being overweight and being obese causes various secondary diseases, e.g. type II. diabetes or cardiovascular diseases. That is why the attempts to stop the increase of prevalence of overweigh...

  3. Ženy v kontextu harmonizace pracovních a rodinných rolí. Mateřství a budování kariéry jako dva hlavní imperativy života současných žen

    OpenAIRE

    Svobodová, Martina

    2011-01-01

    The Bachelor thesis deals with three groups of strategies for reconciliation of work and family roles of contemporary Czech women. The strategies are devided according to three ideal types of women. These ideal types are women who are seeking to harmonize work and family life, than women working only in the sphere of employment, and - finally only women in the sphere of family and household. The text is devoted to various three types of women and to specific groups of women, which are linked ...

  4. Ke zpracování názvů pekařských a cukrářských výrobků v Akademickém slovníku současné češtiny

    Czech Academy of Sciences Publication Activity Database

    Procházková, Barbora

    2016-01-01

    Roč. 99, č. 2 (2016), s. 84-91 ISSN 0027-8203 R&D Projects: GA MK DF13P01OVV011 Keywords : Czech language * dictionary entry * monolingual dictionary * treatment of meaning * genus proximum * distinctive features of meaning Subject RIV: AI - Linguistics

  5. Včelařsky významné pylodárné rostliny jarního a časně letního aspektu na území severní části Blanského lesa

    OpenAIRE

    ZÍDKOVÁ, Michaela

    2013-01-01

    Bachelor work dealt with determination of important plants for bee-keeping from pollen loads and the importance of pollen in honeybee nourishment. Pollen loads were collected from one honeybee colony, which stands to the south from the village of Dobčice in Blanský les Protected Landscape Area. Natural reservations of Chrášťanský vrch and Vysoká Běta are in the distance of flying range from the colony. According to the pollen grains were detected taxons of Blanský les Protected Landscape Area...

  6. Present attitudes of physical education students towards future employment in their profession [Současné názory studentů tělesné výchovy na jejich budoucí zaměstnání v této profesi

    Directory of Open Access Journals (Sweden)

    Magdalena Majer

    2010-06-01

    Full Text Available OBJECTIVE: The aim of the research was to examine empirically the attitudes of physical education students towards their future profession in physical culture and some of their considerations about the choice of their field of study and their opinion about the course of studies. METHODS: The research embraced physical education students studying at the Academy of Physical Education in Cracow. The research took place in two stages (May 2005 and 2008. The first part involved first year students (N = 162, the second the same students then being fourth year students, but only those, who passed all semesters on time (N = 103. The students' attitudes towards their future profession were examined by means of a questionnaire worked out by Grabowski and Skrzypiec. RESULTS: For 45.6% of our respondents the choice of their field of study and prospective profession was determined by positive factors. More than half of the tested students (51.6% chose physical education as their field of study because of their interests and passion. Salary, stability and terms of work are also significant factors. More than half of the respondents declare their willingness to work in their profession after graduation. CONCLUSIONS: From among the values preferred while choosing the physical educator's profession, passion was the main choice of the respondents, regardless of gender. The fact that their studies' positive influence on the respondents' attitudes towards their future profession decrease clearly between the first and the last year of studies, is alarming. It would be advisable to do more detailed research in other schools to verify the educational system and the practical preparation of prospective physical education teachers.[CÍLE: Cílem výzkumu bylo empiricky posoudit názory studentů tělesné výchovy na jejich budoucí zaměstnání v tělesné výchově a některé z důvodů, proč si vybrali tento studijní obor, a jejich názory na průběh studia. METODIKA: Výzkum se zabýval studenty tělesné výchovy na Akademii tělesné výchovy v Krakově. Výzkum se konal ve dvou fázích (květen 2005 a 2008. První část zahrnovala studenty prvních ročníků (N = 162, druhá pak tytéž studenty ve čtvrtém roce studia, ale pouze ty, kteří splnili své studijní povinnosti (N = 103. Názory studentů na jejich budoucí profesi byly zkoumány pomocí dotazníku, jehož autory jsou Grabowski a Skrzypiec. VÝSLEDKY: U 45,6 % respondentů byl výběr studijního oboru a potenciální profese determinován pozitivními faktory. Více než polovina zkoumaných studentů (51,6 % si tělesnou výchovu jako svůj studijní obor vybrala kvůli svým zájmům a nadšení. Dalšími významnými faktory jsou také plat, stabilita a pracovní podmínky. Více než polovina respondentů vyjádřila svou ochotu pracovat v této profesi po ukončení studia. ZÁVĚRY: Mezi hodnotami, jež vedly k výběru profese učitele tělesné výchovy, respondenti nejčastěji uváděli nadšení, a to bez ohledu na pohlaví. Znepokojivé je to, že pozitivní vliv, jaký má studium na názory respondentů, pokud jde o jejich budoucí profesi, mezi prvním a čtvrtým rokem studia evidentně klesá. Bylo by vhodné provést podrobnější výzkum v jiných školách, aby bylo možné ověřit vzdělávací systém a praktickou přípravu potenciálních učitelů tělesné výchovy.

  7. Dva dětské pohřby na pozdně halštatském až časně laténském sídlišti v Dobrovízi, okr. Praha-západ

    Czech Academy of Sciences Publication Activity Database

    Psohlavcová, Monika; Stránská, Petra; Trojánková, Olga

    2017-01-01

    Roč. 21, č. 2 (2017), s. 833-845 ISSN 1214-3553 Institutional support: RVO:67985912 Keywords : infant grave * Late Hallstatt/Early La Tène settlement * import Subject RIV: AC - Archeology, Anthropology, Ethnology OBOR OECD: Archaeology

  8. Květena a vegetace Chráněné krajinné oblasti a Biosférické rezervace Křivoklátsko - současný stav poznání botanického výzkumu

    Czech Academy of Sciences Publication Activity Database

    Kolbek, Jiří

    2011-01-01

    Roč. 2011, č. 31 (2011), s. 147-182 ISSN 0231-5807 Institutional research plan: CEZ:AV0Z60050516 Keywords : vegetation maps * actual vegetation * potentional natural vegetation Subject RIV: EF - Botanics

  9. Hranice jako sociální instituce a geografická kategorie v raně novověké imaginaci prostoru. K současnému výzkumu hranic v raném novověku

    Czech Academy of Sciences Publication Activity Database

    Řezníková, Lenka

    2017-01-01

    Roč. 47, 97/98 (2017), s. 115-131 ISSN 0323-2220 R&D Projects: GA ČR GB14-37038G Institutional support: RVO:67985955 Keywords : Borders * Cartography * Forensic Cartography * Early Modern Period Subject RIV: AB - History OBOR OECD: History (history of science and technology to be 6.3, history of specific sciences to be under the respective headings)

  10. Order of the 22. January 2010 approving the decision n. 2009-DC-0103 made on the 1. of July 2010 by the Nuclear Safety Authority (ASN) defining obligations of quality assurance in radio-therapies defined in R. 1333-59 article of the Public Health Code

    International Nuclear Information System (INIS)

    2009-01-01

    This legal document addresses various aspects and issues: the general requirements of a quality management system, the leadership commitment on this aspect, organisational provisions, the documentation system and its control, the personnel responsibility, the study of risks associated with the radiotherapy process for the patients, the internal declaration of dysfunctions and undesirable situations, improvement actions planning, internal communication, records. These terms are more precisely defined in an appendix

  11. Order of the 29. of January 2010 approving the decision n. 2009-DC-0148 made on the 16. of July 2009 by the Nuclear Safety Authority (ASN) related to the detailed content of information which must be attached to declarations of nuclear activities being applied by paragraphs 1 and 3 of the R. 1333-19 of the Public Health Code

    International Nuclear Information System (INIS)

    2010-01-01

    This legal text defines the detailed content of information which must be attached to declarations required on the account of an article of the French Public Health Code. This information comprises a specific form and a file, the content of which is specified in appendix and may depend on whether it concerns a first declaration or a new declaration after modification. Another appendix defines the documents and information which must be kept at the disposal of authorities and organisations in charge of radioprotection controls and of nuclear safety

  12. Deskrypcja długości i szerokości stóp kobiet i mężczyzn w obciążeniu masą własną, w wieku od 4 do 18 lat w świetle mory projekcyjnej

    Directory of Open Access Journals (Sweden)

    Mirosław Mrozkowiak

    2015-09-01

    Mirosław Mrozkowiak   Uniwersytet Kazimierza Wielkiego, Instytut Kultury Fizycznej, Bydgoszcz e-mail: magmar54@interia.pl, strona: http://wadypostawy.republika.pl   Słowa kluczowe: długość i szerokość stopy.   Streszczenie   Wstęp. Długość i szerokość stopy to cechy, których przyśpieszony wzrost w okresie pokwitania pojawia się najwcześniej. U chłopców przypada na okres między 12,5 a13 r.ż., wg innych doniesień długość i szerokość stopy u chłopców wzrasta do 18 r.ż. Z wiekiem stopa zmienia się z szerokiej i krótkiej u noworodków, do pośredniej u dzieci starszych. Cel. Określenie przebiegu zmian średnich wartości długości i szerokości stóp, okresów gwałtownego wzrostu i spowolnienia przyrostu badanych parametrów w grupie dziewcząt i chłopców w wieku od 4 do 18 lat. Materiał i metodyka. Pomiarami długości i szerokości stóp objęto populację 9804 dziewcząt i 8699 chłopców w wieku od 4 do 18 lat, z losowo wybranych przedszkoli i szkół regionu warmińsko–mazurskiego. Do oceny wykorzystano stanowisko do komputerowej oceny postawy ciała, techniką mory projekcyjnej. Wyniki. Krzywa średnich wartości długości stóp obojga płci ma bardzo zbliżony przebieg  do wykresu właściwej płci. Krzywa rozpoczyna się wartością P: 168,2, L:168,22 mm, kończy P: 238,0, L:233,3 mm. W 14 r.ż. występuje obniżenie wartości do P: 214,68, L:209,91 mm. Szerokość posiada wartość początkową P: 62,99, L:64,92 mm, końcową P: 90,8, L:90,18 mm. W 14 r.ż. występuje załamanie do wartości P: 81,82, L:82,39 mm. Wnioski     1. Przyrost długości i szerokości stóp populacji żeńskiej i męskiej od 4 do 18 r.ż. jest równomiernie intensywny, przy czym w 14 r.ż. następuje spowolnienie przyrostu wielkości badanych cech.     2. Przebieg zmian średnich wielkości długości i szerokości stopy w wieku od 4 do 18 lat regionu warmińsko – mazurskiego nie znajduje w pełni potwierdzenia w badaniach metodą podometryczną.   Keywords: length and width of the foot.   Summary   Admission. The length and width of the foot are the characteristics which accelerated growth during puberty occurs at the earliest. Boys fall for a period of between 12.5 and 13 deposition, according to other reports the length and width of the foot in boys rises to 18 the age of deposition rate varies from a wide and short in newborns, to indirect in children. Aim. Determination of course change of the average values of the length and width of the foot, periods of rapid growth and slow growth of the test parameters in the Group of boys and girls aged from 4 to 18 years. Material and methods. The measurements of length and width of the foot covered the population 9804 girls and 8699 boys aged from 4 to 18 years of age, with a randomly selected kindergartens and schools in the region of Warmia-Mazury. To evaluate the position was used to evaluate computer posture, projection moire. The results. The curve of the average length of the foot of both sexes has a very similar process to the appropriate sex. The curve starts at the value of P: 168,2, L: 168,22 mm, d: 238,0, l: 14 mm 233,3 deposition occurs downgrading to the P: 214,68, L: 209,91 mm. Width of initial value P: 62,99, L: 64,92 mm, final P: 90,8, L: 90,18 mm 14 students at the breakdown occurs to the value of P: 81,82, L:82, 39 mm. Conclusions 1. Increase the length and width of the foot of the female and male populations from 4 to 18 deposition is intense, with evenly deposition growth decelerate 14 size tested characteristics. 2. the Process of change to medium sized length and width of the foot between the ages of 4 to 18 years old in the region of Warmia-Mazury is not fully confirmed in research by podometric method.

  13. Slovníková exemplifikace jako zdroj komiky. Nad příklady elektronické podoby Slovníku současné češtiny (nechybujte.cz, verze 2.0, 2016)

    Czech Academy of Sciences Publication Activity Database

    Lišková, Michaela

    2017-01-01

    Roč. 54, 3/4 (2017), s. 47-50 ISSN 1212-5326 Institutional support: RVO:68378092 Keywords : dictionary * exemplification * comicality * electronic lexicography Subject RIV: AI - Linguistics OBOR OECD: Linguistics

  14. Replika dlažby vyšehradského typu v bazilice sv. Vavřince na Vyšehradě. Vícegenerační archeologický výzkum a současná prezentace archeologické památky

    Czech Academy of Sciences Publication Activity Database

    Boháčová, Ivana

    2013-01-01

    Roč. 29, č. 1 (2013), s. 114-125 ISSN 0231-6056 R&D Projects: GA MK(CZ) DF13P01OVV014 Keywords : Early Middle Ages * sacral architecture * ceramic tiles * heritage care * Prague * presentation Subject RIV: AC - Archeology, Anthropology, Ethnology

  15. Monitorování radiace v časné fázi nehody na jaderném zařízení - analýza všech typů měření použitelných pro korekci modelových předpovědí

    Czech Academy of Sciences Publication Activity Database

    Pecha, Petr; Kuča, P.; Češpírová, I.; Hofman, Radek

    2011-01-01

    Roč. 3, č. 2 (2011), s. 102-125 ISSN 1803-568X R&D Projects: GA MV(CZ) VG20102013018 Institutional research plan: CEZ:AV0Z10750506 Keywords : radiation protection * monitoring network * nuclear emergency Subject RIV: BB - Applied Statistics, Operational Research http://library.utia.cas.cz/separaty/2012/AS/pecha-radiation monitoring in the early phase of an accident at a nuclear facility.pdf

  16. Strnad zahradní (Emberiza hortulana) v České republice: změny početnosti a současné rozšíření v jádrových oblastech

    Czech Academy of Sciences Publication Activity Database

    Šálek, Martin; Beran, V.; Hanzlíková, M.; Kipson, Marina; Molitor, P.; Praus, L.; Procházka, V.; Šimeček, K.; Vít, P.; Zeman, V.

    2016-01-01

    Roč. 52, č. 52 (2016), s. 34-52 ISSN 0231-7796 Institutional support: RVO:68081766 Keywords : agricultural landscape * brown-coal mines * farmland birds * population decline * population size Subject RIV: EG - Zoology

  17. Současné urbanizační pochody a obytné prostředí jako součást kvality života ve vnitřních částech evropských velkoměst

    Czech Academy of Sciences Publication Activity Database

    Klusáček, Petr; Vaishar, Antonín

    2008-01-01

    Roč. 2008, č. 25 (2008), s. 145-157. ISBN 978-80-970076-1-4. ISSN 1210-3519 Institutional research plan: CEZ:AV0Z3086906 Keywords : quality of life * perception * reurbanization * inner zones * target groups Subject RIV: AO - Sociology, Demography

  18. Thermodynamic properties of peptide solutions 20. Partial molar volumes and isothermal compressions for some tripeptides of sequence gly-X-gly (X = gly, ala, leu, asn, thr, and tyr) in aqueous solution at T = 298.15 K and p = (10–120) MPa

    International Nuclear Information System (INIS)

    Hedwig, Gavin R.; Høiland, Harald

    2016-01-01

    Highlights: • Sound speeds were measured for aqueous solutions of some tripeptides at high pressures. • Partial molar volumes and isothermal compressions were derived for T = 298.15 K and p = (10–120) MPa. • The partial molar volumes for non-polar amino acid side-chains decrease with increasing pressure. • The partial molar volumes for polar side-chains do not change significantly with increasing pressure. - Abstract: Sound speeds have been measured for aqueous solutions of six tripeptides of sequence glycyl-X-glycine, where X is one of the amino acids glycine, alanine, leucine, asparagine, threonine, and tyrosine at T = 298.15 K and at the pressures p = (10, 20, 40, 60, 80, 100, and 120) MPa. Using methods described in previous work, these sound speeds were used to derive the partial molar volumes at infinite dilution, V_2"o, the partial molar isentropic compressions at infinite dilution, K_S_,_2"o, and the partial molar isothermal compressions at infinite dilution, K"o_T_,_2 {K"o_T_,_2 = −(∂V_2"o/∂p)_T}, for the tripeptides in aqueous solution at the elevated pressures. The results were used to calculate the partial molar volumes and partial molar isothermal compressions for the various amino acid side-chains over the pressure range p = (10–120) MPa.

  19. Zpráva o předběžné geologické dokumentaci dočasných odkryvů stavby metra linky A na Červeném vrchu v Praze-Vokovicích

    Czech Academy of Sciences Publication Activity Database

    Kraft, P.; Budil, P.; Steinová, M.; Hroch, T.; Tasáryová, Z.; Peršín, J.; Mikuláš, Radek; Kozák, V.

    2011-01-01

    Roč. 2010, - (2011), s. 20-25 ISSN 0514-8057 R&D Projects: GA ČR GA205/09/1521 Institutional research plan: CEZ:AV0Z30130516 Keywords : Middle Ordovician * graptolites * ichnofossils * Šárka Formation * Praha-Vokovice Subject RIV: DB - Geology ; Mineralogy http://www.geology.cz/zpravy/obsah/2010/zpravy-2010-5.pdf

  20. Studium současné vegetace v Súdánu jako důležitý nástroj pro interpretaci archeobotanických výzkumů v severovýchodní Africe // Investigation of recent vegetation in the Sudan as an important tool for further archaeobotanical reconstructions in North-eastern Africa

    Directory of Open Access Journals (Sweden)

    Adéla Pokorná

    2015-06-01

    Full Text Available Attempts at reconstructing past environments are an important part of archaeobotanical investigations. However, any interpretation of archaeobotanical data must be, among other things, based on a detailed knowledge of species behaviour in recent vegetation. To meet this requirement, we studied vegetation in the area surrounding archaeological excavations in the western part of Jebel Sabaloka, near the 6th Nile Cataract in the Sudan, based on sample-plots (relevés in different habitats covering a gradient from dry rocks to the irrigated alluvial plain of the River Nile. The species composition variability clearly corresponds with the environmental gradient of water availability. In addition to that, the vegetation of the irrigated alluvial plain shows a clear difference in the management of the plots (fields versus scrubby edges. Plant species with a narrow niche were selected as potential diagnostic species for certain habitats, in contrast to species with a broad niche. However, we need to be cautious in making generalizations about this finding. Especially for reconstructing the remote past, the knowledge of the local environment would be insufficient. It is generally known that the Holocene climate differed distinctively from that of today. In reconstructing the older phases of the Holocene, it is necessary to investigate recent vegetation in areas situated much further to the south.

  1. Výměna stárnoucí manželky za hezkou mladou kočku aneb K exemplifikaci v Akademickém slovníku současné češtiny

    Czech Academy of Sciences Publication Activity Database

    Lišková, Michaela

    2017-01-01

    Roč. 100, č. 1 (2017), s. 28-36 ISSN 0027-8203 R&D Projects: GA MK DF13P01OVV011 Keywords : dictionary example * exemplification * gender * lexicography * monolingual dictionary * political correctness Subject RIV: AI - Linguistics

  2. Daň z nemovitých věcí v Praze: kvantifikace současné situace a možných změn pomocí dat z daňových přiznání

    Czech Academy of Sciences Publication Activity Database

    Janský, Petr; Šatava, Jiří

    2015-01-01

    Roč. 23, č. 4 (2015), s. 43-62 ISSN 0572-3043 Institutional support: RVO:67985998 Keywords : property tax * tax returns * impact evaluation Subject RIV: AH - Economics http://www.vse.cz/polek/download.php?jnl=aop&pdf=480.pdf

  3. Vztah tělesné aktivity a duševního zdraví – současný pohled kvalitativního výzkumu The physical activity and mental health relationship – a contemporary perspective from qualitative research

    Directory of Open Access Journals (Sweden)

    Brendan Gough

    2006-02-01

    Full Text Available Pozitivní vztah mezi tělesnou aktivitou a duševním zdravím a jeho využití při léčbě duševních chorob jsou známy. Přesto stále nebylo dosaženo shody ohledně mechanismu, který je za tento vztah zodpovědný. Článek zkoumá vztah tělesné aktivity a duševního zdraví kritickým posouzením biochemických, fyziologických a psychologických mechanismů, které mají tento jev vysvětlovat. Z takového posouzení vyplývá, že přes různá navrhovaná vysvětlení panuje v této oblasti jen málo shody, s výjimkou toho, že jde o vztah komplexní a že v zodpovědném mechanismu či zodpovědných mechanismech se pravděpodobně navzájem kombinují prvky psychologické, biochemické i fyziologické. Pokus o lepší pochopení komplexnosti tohoto vztahu doplňují zjištění z kvalitativního výzkumu zkoumajícího vztah z pohledu osob, které prospěšný vliv cvičení na duševní zdraví zažívají. Pomocí metodologie kvalitativního výzkumu zjišťovala studie zkušenosti účastníků cvičebních programů ve Velké Británii. Závěrem je konstatováno, že kvalitativní metodologie zkoumající zkušenosti lidí a prostředky, které je usnadňují, poskytuje další pohled na složitou povahu vztahu tělesné aktivity a duševního zdraví. There is a known positive relationship between physical activity and mental health, and in the treatment and maintenance of mental illness. Despite this relationship however, there still remains a lack of consensus on the mechanism responsible for the relationship. This paper explores the physical activity and mental health relationship by reviewing and critiquing the biochemical, physiological and psychological mechanisms proposed to explain this phenomenon. Through this review it becomes apparent that although there are varied explanations proposed, there is little agreement except that the relationship is complex and the responsible mechanism(s are likely to be interrelated combining the disciplines of psychology, biochemistry and physiology. In an attempt to understand further the complexities of the relationship the paper presents findings from qualitative research that investigated the relationship from the perspectives of people that experience mental health benefits from exercise. Using grounded theory methodology the study investigated the experiences of participants on exercise programmes in the UK. The paper concludes that qualitative methodologies which explore people experiences, and what helps to facilitate them, provide further insight into the interrelated nature of the physical activity and mental health relationship.

  4. K současnému stavu poznání barremsko-aptských amonitů z godulské facie slezské jednotky v Moravských Beskydech (vnější Karpaty, Česká republika)

    Czech Academy of Sciences Publication Activity Database

    Vašíček, Zdeněk

    2009-01-01

    Roč. 2, č. 1 (2009), s. 59-73 ISSN 1803-960X Institutional research plan: CEZ:AV0Z30860518 Keywords : Flysch Carpathians * Hradiště Formation * Lower Cretaceous * ammonites Subject RIV: DB - Geology ; Mineralogy

  5. Nepříliš harmonická realita. Rodičovské kombinace práce a péče v mezích genderové struktury současné české společnosti

    Czech Academy of Sciences Publication Activity Database

    Křížková, Alena

    2007-01-01

    Roč. 8, č. 2 (2007), s. 60-67 ISSN 1213-0028 R&D Projects: GA ČR GA403/05/2474 Institutional research plan: CEZ:AV0Z70280505 Keywords : work / life balance * gender inequalities * labour market Subject RIV: AO - Sociology, Demography www.genderonline.cz

  6. Tak v současných mediálních mluvených rozhovorech: ty zákony co se vztahujou teďkon na nás soukromníky tak to asi budu muset zavřít (1. část)

    Czech Academy of Sciences Publication Activity Database

    Jílková, Lucie

    -, č. 14 (2016), s. 12-33 ISSN 1804-137X R&D Projects: GA ČR GA15-01116S Institutional support: RVO:68378092 Keywords : word tak * DIALOG corpus * mass media dialogue * spoken language * meanings and functions of the Czech word tak Subject RIV: AI - Linguistics OBOR OECD: Linguistics

  7. Social media: the what, the why and the worry. ASN activity on social media. Communications Lessons Learned from the 2014 Radiological Release Event at the Waste Isolation Pilot Plant. Spanish stakeholder for the NEA workshop on stakeholder involvement in nuclear decision making

    International Nuclear Information System (INIS)

    Harrington, Holly; Bouchot, Emmanuel; Runyon, Timothy; Gonzalez Herrero, Eva

    2017-01-01

    Session 8 focused on the media and how it can be utilised to effectively garner stakeholder involvement. It highlighted the changes over the years in how decision makers interact with stakeholders in the nuclear community and the nuances of using the various social media platforms and traditional media outlets. The session had a heavier focus on social media as it is a new and quickly evolving means of engaging the public and other stakeholders. Cases provided insight on current usages, whether in continuing regulatory communication or in response to emergent events. The session included input from regulators, implementers and a media representative sharing the various perspectives on the public communication aspect of stakeholder involvement. They pointed out the various outlets and platforms that can be employed to involve and inform different stakeholders, acknowledging the advantages and disadvantages of each method. Speakers emphasised how important it is that the communication with stakeholders be two-way, allowing thoughts and opinions to be expressed even when they are in stark opposition to nuclear projects or when they are critical of regulatory practices. It is important to consider the stakeholders' perspective and how they may want to be involved in the decision-making process. As stated in the first presentation by Ms Harrington of the US Nuclear Regulatory Commission and echoed throughout the session, stakeholders in general want information that will update them as to current activities, how they could be affected and how they can possibly influence the process. During an emergency, there may be special risk communication needs. The strategic use of social media platforms can assist organisations in engaging stakeholders and provide the information and interaction they may want. Social media includes web sites and more specifically, applications that allow the user to create and share information. It was recognised during the presentations that the objectives in reaching stakeholders through media are not static. There is the ongoing relationship and trust building that takes place (including with traditional journalists, as outlined by Ms Gonzalez Herrero) when there are no momentous events. Organisations should have this relationship in place before the need arises to communicate with all stakeholders when an incident occurs or during an emergency. Goals can include making expert content better known, or demonstrating positive values of openness and transparency. The organisation must identify its goals in using the various media outlets to engage its stakeholders. Social media is one of many tools that can be used to address issues and gaps strategically but should not replace existing methods of information dissemination and should complement other forms of stakeholder involvement. Social media allows the magnification of a message simultaneously on various platforms to share the organisation's perspective. Different platforms target different audiences and should be used in integrated and compatible ways. Though a message can be broadcast directly, it is still important to be engaged with traditional media outlets. The reach of information will be increased in turn via these outlets' own social media accounts. Though social media garnered a large portion of the session, this tool is still in the process of being fully embraced and utilised strategically to involve stakeholders during the various stages of decision making. The case study offered by Mr Runyon of the US Department of Energy's Waste Isolation Pilot Plant showed that time is needed to build up a rich, responsive communications practice as well as audience ('followers'). It is imperative to adapt to the specific rules of social media, which use a conversational tone and require fast and attractive updating. Mr Bouchot of France's Nuclear Safety Authority outlined the various characteristics of each social media platform and the importance of knowing these features to engage and inform stakeholders. For instance, Twitter offers direct interaction with influential followers who can expand the reach of information. This was illustrated by the effective spread in France of authoritative and correct information about an iodine distribution campaign through 're-tweets' by other Twitter users. When a major event occurs, there is a need to balance speed, accuracy and comprehensiveness in putting out the information. Fast-paced use of the multiplying power of the social media can be beneficial in getting information out to all stakeholders but it can also be a double-edged sword. As highlighted in the first presentation, if mistakes are made, they are magnified and the content may stay on the web forever, which presents a tremendous challenge. Resources can be directed to correcting an issue but it is important to understand that it is impossible to correct everything. A strategic approach to righting incorrect information on social media platforms is to allow the conversation to range freely, permitting external persons to the organisation to challenge what is presented and allowing other stakeholders to correct mistakes, in an interactive manner

  8. Současná mezinárodněprávní úprava použití ozbrojené síly - zákaz použití síly a jeho nesporné výjimky

    Czech Academy of Sciences Publication Activity Database

    Popenková, Monika

    2008-01-01

    Roč. 147, č. 8 (2008), s. 862-898 ISSN 0231-6625 R&D Projects: GA AV ČR KJB700680601 Institutional research plan: CEZ:AV0Z70680506 Keywords : the United Nations Charter * prohibition of force application * self-defence right Subject RIV: AG - Legal Sciences

  9. Vliv časné nutriční podpory a rehabilitace během akutního onemocnění na následnou soběstačnost a tělesnou kondici u geriatrických pacientů - prospektivní dlouhodobá studie

    OpenAIRE

    Dědková, Zuzana

    2018-01-01

    - Effect of clinical nutrition and physiotherapy in acutely ill geriatric patients on self-sufficiency and physical condition - prospective longitudinal study Background & aims: Acute internal disease of the elderly often leads to loss of muscle mass and self-sufficiency. Reversal of muscle tissue, especially in geriatric patients, is very difficult and thus can an acute illness leads to necessity a second person helping a form of subsequent social care at home or in institutions aftercare. T...

  10. Rozdíly v klinickém obraze a laboratorních nálezech v časné fázi aseptického zánětu CNS dle vyvolávajícího agens

    Czech Academy of Sciences Publication Activity Database

    Heinige, P.; Fajt, M.; Šilhánková, I.; Halašková, P.; Jiroušová, M.; Matějčková, Eva; Ješina, Pavel

    2006-01-01

    Roč. 61, č. 5 (2006), s. 273-273 ISSN 0069-2328. [Český pediatrický kongres s mezinárodní účastí /7./. 08.06.2006-11.06.2006, Praha] Keywords : CNS * aseptic inflammation Subject RIV: FG - Pediatrics

  11. Enhanced extracellular production of L-asparaginase from Bacillus subtilis 168 by B. subtilis WB600 through a combined strategy.

    Science.gov (United States)

    Feng, Yue; Liu, Song; Jiao, Yun; Gao, Hui; Wang, Miao; Du, Guocheng; Chen, Jian

    2017-02-01

    L-asparaginase (EC 3.5.1.1, ASN) exhibits great commercial value due to its uses in the food and medicine industry. In this study, we reported the enhanced expression of type II ASN from Bacillus subtilis 168 in B. subtilis WB600 through a combined strategy. First, eight signal peptides (the signal peptide of the ASN, ywbN, yvgO, amyE, oppA, vpr, lipA, and wapA) were used for ASN secretion in B. subtilis by using Hpa II promoter, respectively. The signal peptide wapA achieved the highest extracellular ASN activity (28.91 U/mL). Second, Hpa II promoter was replaced by a strong promoter, P43 promoter, resulting in 38.1 % enhanced ASN activity. By two rounds of error-prone PCR mutation, the P43 promoter variants with remarkably enhanced strength (D7, E2, H6, B2, and F3) were identified. B2 (-28: A → G, -13: A → G) achieved ASN activity up to 51.13 U/mL. Third, after deletion of the N-terminal 25-residues, ASN activity reached 102.41 U/mL, which was 100 % higher than that of the intact ASN. At last, the extracellular ASN of the B. subtilis arrived at 407.6 U/mL (2.5 g/L of ASN protein) in a 3-L bioreactor by using a fed-batch strategy. The purified ASN showed maximal activity at 65 °C and its half-life at 65 °C was 61 min. The K m and k cat of the ASN were 5.29 mM and 54.4 s -1 , respectively. To the best of our knowledge, we obtained the highest yield of ASN in a food-grade host ever reported, which may benefit the industrial production and application of ASN.

  12. Measurement of Fructose-Asparagine Concentrations in Human and Animal Foods.

    Science.gov (United States)

    Wu, Jikang; Sabag-Daigle, Anice; Metz, Thomas O; Deatherage Kaiser, Brooke L; Gopalan, Venkat; Behrman, Edward J; Wysocki, Vicki H; Ahmer, Brian M M

    2018-01-10

    The food-borne bacterial pathogen, Salmonella enterica, can utilize fructose-asparagine (F-Asn) as its sole carbon and nitrogen source. F-Asn is the product of an Amadori rearrangement following the nonenzymatic condensation of glucose and asparagine. Heating converts F-Asn via complex Maillard reactions to a variety of molecules that contribute to the color, taste, and aroma of heated foods. Among these end derivatives is acrylamide, which is present in some foods, especially in fried potatoes. The F-Asn utilization pathway in Salmonella, specifically FraB, is a potential drug target because inhibition of this enzyme would lead to intoxication of Salmonella in the presence of F-Asn. However, F-Asn would need to be packaged with the FraB inhibitor or available in human foods. To determine if there are foods that have sufficient F-Asn, we measured F-Asn concentrations in a variety of human and animal foods. The 400 pmol/mg F-Asn found in mouse chow is sufficient to intoxicate a Salmonella fraB mutant in mouse models of salmonellosis, and several human foods were found to have F-Asn at this level or higher (fresh apricots, lettuce, asparagus, and canned peaches). Much higher concentrations (11 000-35 000 pmol/mg dry weight) were found in heat-dried apricots, apples, and asparagus. This report reveals possible origins of F-Asn as a nutrient source for Salmonella and identifies foods that could be used together with a FraB inhibitor as a therapeutic agent for Salmonella.

  13. Role of pH in determining the cell-type-specific residual activity of glucocerebrosidase in type 1 Gaucher disease

    NARCIS (Netherlands)

    van Weely, S.; van den Berg, M.; Barranger, J. A.; Sa Miranda, M. C.; Tager, J. M.; Aerts, J. M.

    1993-01-01

    The properties of control and 370Asn-->Ser glucocerebrosidase, the frequently encountered mutated form of the enzyme in type 1 Gaucher disease, were studied in vitro as well as in situ. The catalytic properties of purified 370Asn-->Ser glucocerebrosidase were highly dependent on the assay

  14. 32 CFR 775.12 - Delegation of authority.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 5 2010-07-01 2010-07-01 false Delegation of authority. 775.12 Section 775.12... FOR IMPLEMENTING THE NATIONAL ENVIRONMENTAL POLICY ACT § 775.12 Delegation of authority. (a) The ASN... instruction as deemed appropriate. (b) The ASN (RD&A) delegation of authority for approval and signature of...

  15. Application of MALDI-TOF mass spectrometry for study on fibrillar and oligomeric aggregates of alpha-synuclein

    NARCIS (Netherlands)

    Severinovskaya, O. V.; Kovalska, V B; Losytskyy, M Yu; Cherepanov, V. V.; Subramaniam, V.; Yarmoluk, S M

    2014-01-01

    Aim. To study the α-synuclein (ASN) aggregates of different structural origin, namely amyloid fibrils and spherical oligomers, in comparison with a native protein. Methods. MALDI TOF mass spectrometry and atomic force microscopy (AFM). Results. The mass spectra of native and fibrillar ASN have

  16. Problems of Journalism; Proceedings of the 1975 Annual Convention of the American Society of Newspaper Editors (Washington, D.C., April 16-18, 1975).

    Science.gov (United States)

    American Society of Newspaper Editors, Easton, PA.

    This document reports the 1975 proceedings of the American Society of Newspaper Editors (ASNE) convention held in Washington, D.C., April 16-18. The contents include a list of officers and directors, past presidents of the society, and a copy of the ASNE Code of Ethics. Also contained in the document are reports on such individual sessions as…

  17. The key events of 2012

    International Nuclear Information System (INIS)

    2013-01-01

    The article reviews the main events or changes or issues that occurred in 2012 in France in the different sectors of activities of the ASN (control, public information, management of accidental situations, and international cooperation) or that had an impact on the activities of ASN (changes in national or european regulations for instance)

  18. Oxytocin biotransformation in the rat limbic brain

    NARCIS (Netherlands)

    Burbach, J.P.H.; Schotman, P.; Kloet, E.R. de

    2006-01-01

    Two peptide fragments of oxytocin were isolated by high-pressure liquid chromatography from digests of oxytocin obtained after exposure to a SPM preparation of the rat limbic brain. The structures of these peptides, being Gln-Asn-Cys(O)x-Pro-Leu-GlyNH2 and Gln-Asn-Cys(-S-S-Cys)-Pro-Leu-GlyNH2, were

  19. Calcium regulates the expression of a Dictyostelium discoideum ...

    Indian Academy of Sciences (India)

    In a screen for calcium-regulated gene expression during growth and development of Dictyostelium discoideum we have identified an asparaginyl tRNA synthetase (ddAsnRS) gene, the second tRNA synthetase gene identified in this organism. The ddAsnRS gene shows many unique features. One, it is repressed by ...

  20. Deamidation Reactions of Asparagine- and Glutamine-Containing Dipeptides Investigated by Ion Spectroscopy

    NARCIS (Netherlands)

    Kempkes, L.J.M.; Martens, J.; Grzetic, J.; Berden, G.; Oomens, J.

    2016-01-01

    Deamidation is a major fragmentation channel upon activation by collision induced dissociation (CID) for protonated peptides containing glutamine (Gln) and asparagine (Asn) residues. Here, we investigate these NH3-loss reactions for four Asn- and Gln-containing protonated peptides in terms of the

  1. Xeroderma pigmentosum group D polymorphisms and esophageal cancer susceptibility: a meta-analysis based on case-control studies.

    Science.gov (United States)

    Yang, Rong; Zhang, Chong; Malik, Armah; Shen, Zhi-Da; Hu, Jian; Wu, Yi-He

    2014-11-28

    To clarify the effects of the xeroderma pigmentosum group D (XPD) Asp312Asn and Lys751Gln gene polymorphisms on the risk of esophageal cancer (EC). A computerised literature search was conducted to identify the relevant studies from the PUBMED and EMBASE databases, reviews, and reference lists of relevant articles. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the associations between the XPD Asp312Asn and/or Lys751Gln polymorphisms and EC susceptibility. Statistical analyses were performed using the software Stata 12.0. A fixed or random effects model was selected based on a heterogeneity test. Publication bias was estimated using funnel plots and Egger's linear regression method. Subgroup analyses were performed based on histological type and ethnicity. Thirteen case-control studies with a total of 10 comparisons for the Asp312Asn polymorphism, including 2373 cases and 3175 controls, and 15 comparisons for the Lys751Gln polymorphism, including 3226 cases and 5237 controls, were recruited for the meta-analysis. In terms of the XPD Asp312Asn polymorphism, significantly increased EC risks were identified in the Asp/Asn vs Asp/Asp comparison (OR = 1.17, 95%CI: 1.02-1.33, P = 0.03) and in the dominant-model comparison (Asn/Asn+Asp/Asn vs Asp/Asp: OR = 1.18, 95%CI: 1.04-1.34, P = 0.01). However, no significant associations were found in the Asn/Asn vs Asp/Asp comparison (OR = 1.30, 95%CI: 1.00-1.70, P = 0.05) or in the recessive-model comparison (Asn/Asn vs Asp/Asn + Asp/Asp: OR = 1.17, 95%CI: 0.91-1.50, P = 0.22). In terms of the XPD Lys751Gln polymorphism, a significant association with EC susceptibility was found under the recessive model (Gln/Gln vs Lys/Gln+Lys/Lys: OR = 1.21, 95%CI: 1.02-1.43, P = 0.03). However, no associations were identified in the other comparisons (co-dominant model: Lys/Gln vs Lys/Lys: OR = 1.11, 95%CI: 0.94-1.31, P = 0.20; Gln/Gln vs Lys/Lys: OR = 1.31, 95%CI: 0.98-1.75, P = 0.07; dominant model: OR = 1.14, 95%CI

  2. Primary structure of human alpha 2-macroglobulin. I. Isolation of the 26 CNBr fragments, amino acid sequence of 13 small CNBr fragments, amino acid sequence of methionine-containing peptides, and alignment of all CNBr fragments

    DEFF Research Database (Denmark)

    Sottrup-Jensen, Lars; Stepanik, T M; Jones, C M

    1984-01-01

    -775). These fragments account for 603 of the 1451 residues of the subunits of alpha 2-macroglobulin. CB2 contains two glucosamine-based carbohydrate groups attached to Asn-23 and Asn-38, and one internal disulfide bridge connecting Cys-16 with Cys-54. CB6 contains one glucosamine-based carbohydrate group attached...... to Asn-1 and two internal disulfide bridges (Cys-5 bound to Cys-53 and Cys-23 bound to Cys-41, respectively); Cys-32 is bound to Cys-16 in CB8. CB7 contains two glucosamine-based carbohydrate groups attached to Asn-78 and Asn-92, CB8 contains 1 Cys residue (Cys-16), bridged to Cys-32 of CB6. CB11...

  3. The frequency of genotypes for the SNP Ser/Ser in the studied population of Albanian women is higher in the Balkan region

    Directory of Open Access Journals (Sweden)

    Zafer Gashi

    2016-08-01

    Full Text Available In women undergoing natural cycles, just one oocyte is usually selected for ovulation, yet routine clinical techniques to support the development of multiple follicles using additional gonadotrophins result in numerous ovulations. Several parameters have been postulated as predictors of ovarian response (inhibin B, 17-β-estradiol and antiMüllerian hormone. Nevertheless, the FSH level on the day 3 of menstrual cycle remains, the most widely used biomarker due to its low cost, although, the genetic background of individuals seems to determine the response of patients to rFSH stimulation better than the stimulation design. Consequently, the variants of FSHR were explored and they may be involved in the role of FSH receptor in mediated signal transduction and with ovarian response in infertile women submitted to ovarian stimulation. In this study we examined, for the first time, the prevalence of genotype variants Asn680Ser in population Albanian women from Kosovo Dukagjin region who took part in IVF / ICSI program. The frequencies of the Asn680Ser genotype variants were as follows: Asn/Asn 22.1%, Asn/Ser 47.1%, and Ser/Ser 30.8%, respectively (Table 1. bE2 levels between the three genotype variants showed slight but statistically significant difference (p= 0.0308. No difference was also found between the genotype groups either in terms of AFC, amount of the FSH required for ovulation induction, stimulation length days, number of dominant follicles, oocyte retrieval number or endometrial thickness (Table 2. BMI was significantly higher in the Ser/Ser group as compared to those from the Asn/Ser or the Asn/Asn group (p= 0.0010 (Table 2. In the study population of Albanian women Dukagjin region of Kosovo had a higher incidence of Ser / SER genotype compared to Asn / Asn genotype. Our research results in the Albanian population differ from published data for other ethnic groups in the Balkans.

  4. Measurement of fructose-asparagine concentrations in human and animal foods.

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Jikang; Sabag-Daigle, Anice; Metz, Thomas O.; Kaiser, Brooke LD; Gopalan, Venkat; Behrman, Edward J.; Wysocki, Vicki H.; Ahmer, Brian

    2018-01-10

    The food-borne bacterial pathogen, Salmonella enterica, can utilize fructose-asparagine (F-Asn) as the sole carbon and nitrogen source. F-Asn is the product of an Amadori rearrangement following the non-enzymatic condensation of glucose and asparagine. Heating converts F-Asn via complex Maillard reactions to a variety of molecules which contribute to the color, taste, and aroma of heated foods. Among these is acrylamide, which is present in some foods, especially in fried potato products. The F-Asn utilization pathway in Salmonella, specifically FraB, is a potential drug target because inhibition of this enzyme would lead to intoxication of Salmonella in the presence of F-Asn. However, F-Asn would need to be packaged with the FraB inhibitor, or available in human foods. To determine if there are foods that have sufficient F-Asn, we measured F-Asn concentrations in a variety of human and animal foods. The 399 pmol/mg F-Asn found in mouse chow is sufficient to intoxicate a Salmonella fraB mutant in mouse models of salmonellosis, and several human foods were found to have this concentration or higher (fresh apricots, lettuce, asparagus, and in canned peaches). Much higher concentrations (11,000 to 35,000 pmol/mg dry weight) were found in heat-dried apricots, apples, and asparagus. This report clarifies the origins of F-Asn as a nutrient source for Salmonella, and identifies foods that could be used in concert with a FraB inhibitor as a therapeutic agent for Salmonella.

  5. Multi-spectroscopic studies on the interaction of human serum albumin with astilbin: Binding characteristics and structural analysis

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Jin; Li, Shuang; Peng, Xialian; Yu, Qing [Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Department Chemistry and Chemical Engineering, Guangxi Normal University, Ministry of Education of China, Guilin 541004 (China); Bian, Hedong, E-mail: gxnuchem312@yahoo.com.cn [Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Department Chemistry and Chemical Engineering, Guangxi Normal University, Ministry of Education of China, Guilin 541004 (China); Huang, Fuping [Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Department Chemistry and Chemical Engineering, Guangxi Normal University, Ministry of Education of China, Guilin 541004 (China); Liang, Hong, E-mail: lianghongby@yahoo.com.cn [Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Department Chemistry and Chemical Engineering, Guangxi Normal University, Ministry of Education of China, Guilin 541004 (China)

    2013-04-15

    Five spectroscopic techniques were used to investigate the interaction of astilbin (ASN) with human serum albumin (HSA). UV–vis absorption measurements prove that ASN–HSA complex can be formed. The analysis of fluorescence spectra reveal that in the presence of ASN, quenching mechanism of HSA is considered as static quenching. The quenching rate constant k{sub q}, K{sub SV} and the binding constant K were estimated. According to the van't Hoff equation, the thermodynamic parameters enthalpy change (ΔΗ) and entropy change (ΔS) were calculated to be −12.94 kJ mol{sup −1} and 35.92 J mol{sup −1} K{sup −1}, respectively. These indicate that the hydrophobic interaction is the major forces between ASN and HSA, but the hydrogen bond interaction cannot be excluded. The changes in the secondary structure of HSA which was induced by ASN were determined by circular dichroism (CD), Fourier transform infrared spectroscopy (FT-IR) and Raman spectroscopy. -- Graphical abstract: In this paper, the interaction of HSA with ASN was systematically studied under simulated physiological conditions by using UV–vis absorption, CD, FT-IR, fluorescence and Raman spectroscopic approaches. The quenching constant k{sub q}, K{sub SV} and the binding constant K were estimated. The changes in the secondary structure of HSA were studied by Circular dichroism (CD), Fourier transform infrared spectroscopy (FT-IR) and Raman spectroscopy. The UV–visible absorption spectra of HSA in the absence and presence of different concentration of ASN (1) and fluorescence spectra of HSA in the absence and the presence of ASN (2). Highlights: ► Interaction of ASN and HSA has been studied by five spectroscopic techniques. ► Hydrophobic interaction is the major forces between ASN and HSA. ► Binding of ASN induced the changes in the secondary structure of HSA.

  6. Investigating the association between polymorphism of follicle-stimulating hormone receptor gene and ovarian response in controlled ovarian hyperstimulation

    Directory of Open Access Journals (Sweden)

    Mohammad Hasan Sheikhha

    2011-01-01

    Full Text Available Aim : The aim of the study was to investigate the association between follicle-stimulating hormone receptor (FSHR gene polymorphism at Position 680 and the outcomes of controlled ovarian hyperstimulation for in vitro fertilization and embryo transfer (IVF-ET in infertile women. Materials and Methods : One hundred and eight patients under 35 years of age who underwent IVF-ET procedures were included in this study. The hormonal profile and treatment of all patients were analyzed and FSHR polymorphism was examined by polymerase chain reaction-restriction fragment length polymorphism. Women from all groups were classified based on polymorphisms at Position 680, occupied either by asparagines (Asn or serine (Ser as Asn/Asn, Asn/Ser, and Ser/Ser genotype. Result : Our study showed that all patients in the Asn/Asn group were normal responders and in the Asn/Ser group 64.8% were normal responders and 21.1% and 14.1% were poor and hyper responders respectively. In the Ser/Ser group we did not have normal responders and 46.7% of these patients were poor responders and 53.3% were hyper responders. Conclusion : FSH receptor polymorphism is correlated with response to ovarian stimulation.

  7. Reactive inspection and the possible consequences: the situation with Onera in Toulouse

    International Nuclear Information System (INIS)

    Luc, J.Ch.

    2009-01-01

    Controlling the respect of occupational radiation protection rules is one of ASN's core missions. In order to achieve this, ASN realizes hundreds of inspections of nuclear activities each year. Some of them take place after an event (suspicion of incident or accident) ASN has been informed of, in order to clarify the causes and circumstances of the event. According to the severity of the faults discovered, ASN can impose administrative and penal sanctions against the person in charge of the nuclear activity: e.g. stopping the activity and informing the public prosecutor of the established infringements of the law. This article gives a concrete illustration of a 'reactive inspection' and its consequences concerning an accident which occurred on Wednesday 12 March 2008 in an irradiation installation in Toulouse. A worker of the Hirex Engineering company working for the Onera company was incidentally exposed during several minutes to a cobalt-60 source of high activity (10 TBq). The dose received by this worker was evaluated to be 120 mSv. ASN rated this incident at INES level 3. ASN's inspectors and labour inspector led a 'reactive inspection' on 17 March. Many technical and organisational defaults, involving three companies, were detected. A report was sent to the public prosecutor on 20 October 2008. Moreover, ASN stopped the use of irradiation installation and asked Onera company to modify and secure the installation before restarting. As for now, the installation is still stopped. (author)

  8. El amor de lejos de Jaufré Rudel y sus transformaciones medievales: mito, vida, cuento y narración novelesca

    OpenAIRE

    Carmona Fernández, Fernando

    2013-01-01

    La vida de Jaufré Rudel originada en su poesía dará lugar a distintas modalidades narrativas. Sus manifestaciones literarias medievales en interrelación particularmente con la leyenda de Tristán y también con relatos como Guillaume de Dole y Lai de l’ombre de Jean Renart, Chastelaine de Vergi, Roman du Castelain de Coucy y cuentos 44 y 77 del Decamerón, entre otros textos, hace que el tema del amor de lejos constituya con todos ellos, a fnales del siglo XIII, la mod...

  9. Ubiquitin Proteasome System in Parkinson Disease: a keeper or a witness?

    Science.gov (United States)

    Martins-Branco, Diogo; Esteves, Ana R.; Santos, Daniel; Arduino, Daniela M.; Swerdlow, Russell H.; Oliveira, Catarina R.; Januario, Cristina; Cardoso, Sandra M.

    2014-01-01

    Objective The aim of this work was to evaluate the role of Ubiquitin-Proteasome System (UPS) on mitochondrial-driven alpha-synuclein (aSN) clearance in in vitro, ex vivo and in vivo Parkinson disease (PD) cellular models. Method We used SH-SY5Y ndufa2 knock-down (KD) cells, PD cybrids and peripheral blood mononuclear cells (PBMC) from patients meeting the diagnostic criteria for PD. We quantified aSN aggregation, proteasome activity and protein ubiquitination levels. In PBMC of PD patients population we evaluated aSN levels in plasma and the influence of several demographic characteristics in the above mentioned determinations. Results We found that ubiquitin-independent proteasome activity was up-regulated in SH-SY5Y ndufa2 KD cells while a down regulation was observed in PD cybrids and PBMC. Moreover, we observed an increase in protein ubiquitination that correlates with a decrease in ubiquitin-dependent proteasome activity. Accordingly, proteasome inhibition prevented ubiquitin-dependent aSN clearance. Ubiquitin-independent proteasome activity was positively correlated with ubiquitination in PBMC. We also report a negative correlation of chymotrypsin-like activity with age in control and late-onset PD groups. Total ubiquitin content is positively correlated with aSN oligomers levels, which leads to an age-dependent increase of aSN ubiquitination in LOPD. Moreover, aSN levels are increased in the plasma of PD patients. Interpretation aSN oligomers are ubiquitinated and we identified an ubiquitin-dependent clearance insufficiency with accumulation of both aSN and ubiquitin. However, SH-SY5Y ndufa2 KD cells showed a significant up-regulation of ubiquitin-independent proteasomal enzymatic activity that could mean a cell rescue attempt. Moreover, we identified that UPS function is age-dependent in PBMC. PMID:22921536

  10. Operational monitoring of acoustic sensor networks

    Directory of Open Access Journals (Sweden)

    Boltenkov V.A.

    2015-06-01

    Full Text Available Acoustic sensor networks (ASN are widely used to monitor water leaks in the power generating systems. Since the ASN are used in harsh climatic conditions the failures of microphone elements of ASN are inevitable. That's why the failure detection of ASN elements is a problem of current interest. Two techniques of operational monitoring ASN are developed. Both of them are based on the placement of the test sound source within a network. The signal processing for ASN sensors had to detect the failed element. Techniques are based time difference of arrival (TDOA estimating at the each pair of ASN elements. TDOA estimates as argmaximum of cross-correlation function (CCF for signals on each microphone sensors pair. The M-sequence phase-shift keyed signal is applied as a test acoustic signal to ensure high accuracy of the CCF maximum estimation at low signal/noise ratio (SNR. The first technique is based on the isolation principle for TDOA sum at three points. It require to locate the test sound source in the far field. This is not always possible due to technological reasons. For the second proposed technique test sound source can be located near the ASN. It is based on a system of hyperbolic equations solving for each of the four elements of the ASN. Both techniques has been tested in the computer imitation experiment. It was found that for the SNR to –5 dB both techniques show unmistakable indicators of control quality. The second method requires significantly more time control.

  11. Predictive value of XPD polymorphisms on platinum-based chemotherapy in non-small cell lung cancer: a systematic review and meta-analysis.

    Directory of Open Access Journals (Sweden)

    Mantang Qiu

    Full Text Available BACKGROUND: The correlation between xeroderma pigmentosum group D (XPD polymorphisms (Lys751Gln and Asp312Asn and clinical outcomes of non-small cell lung cancer (NSCLC patients, who received platinum-based chemotherapy (Pt-chemotherapy, is still inconclusive. This meta-analysis was aimed to systematically review published evidence and ascertain the exact role of XPD polymorphisms. METHODS: Databases of MEDLINE and EMBASE were searched up to April 2013 to identify eligible studies. A rigorous quality assessment of eligible studies was conducted according the Newcastle-Ottawa Quality Assessment Scales. The relationship between XPD polymorphisms and response to Pt-chemotherapy and survival was analyzed. RESULTS: A total of 22 eligible studies were included and analyzed in this meta-analysis. The overall analysis suggested that the XPD Lys751Gln polymorphism was not associated with response to Pt-chemotherapy or survival. However, the XPD 312Asn allele was significantly associated with poor response to Pt-chemotherapy compared with the Asp312 allele (Asn vs. Asp: OR = 0.435, 95% CI: 0.261-0.726. Additionally, the variant genotype of XPD Asp312Asn polymorphism was associated with favorable survival in Caucasian (AspAsn vs. AspAsp: HR = 0.781, 95% CI: 0.619-0.986 but unfavorable survival in Asian (AspAsn+AsnAsn vs. AspAsp: HR = 1.550, 95% CI: 1.038-2.315. CONCLUSIONS: These results suggest that XPD Asp312Asn polymorphism may function as a predictive biomarker on platinum-based chemotherapy in NSCLC and further studies are warranted.

  12. Ropný průmysl Jižního Súdánu jako faktor ovlivňující současné dění v Jižním Súdánu a vztahy mezi Súdánem a Jižním Súdánem

    OpenAIRE

    Hanzalová, Kateřina

    2014-01-01

    Petroleum is one of the most important commodities traded on the international markets. World oil reserves are limited and therefore becoming increasingly important. Sudan and South Sudan control the sixth highest African oil reserves. However, both states have to face a number of problems which the petroleum industry brings. The bachelor project primarily focuses on South Sudan and its petroleum industry. The aim of the project is to describe how the petroleum industry of South Sudan influen...

  13. Dočasná symetrie zdravých a protetických končetin během chůze osoby s transtibiální amputací s různým protetickým zařazením Temporal symmetry of sound and prosthetic limbs during transtibial amputee gait with various prosthetic alignment

    Directory of Open Access Journals (Sweden)

    Miroslav Janura

    2007-01-01

    Full Text Available Termín "chůze" označuje základní pohyb, jehož pomocí se jednotlivec pohybuje z jednoho místa na druhé. U osob s postižením dolních končetin je tato aktivita částečně či zcela redukována. Během biomechanického šetření chůze u osob s různým postižením se také zkoumala symetrie zatížení dolních končetin. Určité množství biomechanických studií se zaměřuje na kinematické a dynamické proměnné cyklu chůze u osob s transtibiální amputací (Bateni & Olney, 2002; Thomas et al., 2000; Perry, 2004. Zajímavým problémem je hodnocení symetrie chůze mezi osobami s transtibiální amputací a skupinami zdravých jedinců (Winter & Sienko, 1988; Dingwell, Davis, & Frazier, 1996. Miff et al. (2005 srovnává dočasnou symetrii u zdravé skupiny s hodnotami u skupiny osob s transtibiální amputací během počáteční a konečné fáze chůze. Nolan et al. (2003 se zajímal o změny v symetrii chůze ovlivněné rychlostí chůze u osob s transtibiální a transfemorální amputací ve srovnání se zdravou skupinou. Symetrii chůze u osob s transtibiální amputací, které na počátku rehabilitace nosily dvě různé protetické nohy, zkoumal Marinakis (2004. Úplná symetrie chůze není vždy žádoucí. Lidský systém s velkou strukturální asymetrií v neuromuskulární kosterní soustavě (jedna končetina byla amputována nemůže optimálně fungovat, když je chůze symetrická (Winter & Sienko, 1988. V tomto případě je lepší nesymetrická chůze s omezením reziduálního systému a mechaniky protézy. U osob, které podstoupily amputaci, může asymetrická chůze být nástrojem, jenž chrání pahýl postižené nohy. Lze říci, že asymetrie chůze je relevantním měřítkem pro zkoumání charakteristiky chůze osob s amputací a stanovení jejich sklonu k budoucí společné bolesti a degeneraci (Nolan et al., 2003. Pro chůzi osob s amputací je velmi důležitý výběr vhodné protézy. Vliv protetické nohy na proměnné chůze ve skupině těchto probandů zkoumal Gitter et al. (1991. Efektivnost chůze je také významně ovlivněna nastavením protézy nebo protetické nohy. Rehabilitaci osoby s amputací lze považovat za úspěšnou pouze tehdy, pokud tato osoba považuje protézu během chůze za estetickou a pohodlnou. Nastavení protézy musí vyhovovat potřebám postiženého (Fridman, Ona, & Isakov, 2003. Když protetická noha není optimálně vyladěna, může se tento fakt projevit v různých cyklech chůze. Během analýzy chůze je protéza úmyslně "neoptimálně" nastavena, aby se vyvolaly skryté následky. Fridman, Ona a Isakov (2003 pozorovali vliv protetické nohy umístěné ve vnější rotaci. Schmalz, Blumentritt a Jarasch (2002 použili sagitální posun nohy vpřed a vzad a nastavení nohy do plantární a dorzální flexe. Blumentritt et al. (1999 se zaměřil na účinky nastavení protézy v sagitální rovině na velikost zatížení kolenního kloubu u stojící osoby s transtibiální amputací. Nastavení protézy u osob s amputací dolní končetiny má pomoci zvýšit pohodlí zdravé nohy a maximalizovat možnosti chůze těchto osob. V rámci biomechanické analýzy chůze je důležité prošetřit nejen vztah mezi nastavením protetické nohy a protetické končetiny, ale také vztah mezi tímto nastavením a aktivitou druhé, zdravé končetiny (Pinzur et al., 1995. V jiných studiích se objevuje důraz na zdravou končetinu (Nolan & Lees, 2000; Hurley et al., 1990, ale v nich není řešena otázka různých nastavení protéz. Tento vztah mezi zdravou a protetickou končetinou může být kvantitativní, měřený dle symetrie nebo asymetrie měřených proměnných. The term "gait" refers to fundamental locomotion by means of which the individual moves him/herself from one place to another. For people afflicted by some involvement of the lower limbs, this activity is partially or completely reduced. In the course of the biomechanical investigation of gait in subjects with various afflictions, the symmetry of lower limb loading is also the object of research. A certain number of biomechanical studies are focussed on the kinematic and dynamic variables of the gait cycle in subjects with transtibial amputation (Bateni & Olney, 2002; Thomas et al., 2000; Perry, 2004. An interesting problem is the evaluation of gait symmetry between a transtibial amputee and groups of healthy persons (Winter & Sienko, 1988; Dingwell, Davis, & Frazier, 1996. Miff et al. (2005 compared temporal symmetries in a healthy group with the values in a transtibial amputee group during gait initiation and termination. Nolan et al. (2003 took an interest in changes in gait symmetry influenced by gait speed in transtibial and transfemoral amputees in comparison with a healthy group. The interlimb gait symmetry of transtibial amputees wearing two different prosthetic feet in the early rehabilitation stage was investigated by Marinakis (2004. Full symmetry in one's gait is not always desirable. The human system, with its major structural asymmetries in the neuromuscular skeletal system (one limb has been amputated cannot perform optimally when the gait is symmetrical (Winter & Sienko, 1988. Non symmetrical gait performance, with constraints of its residual system and the mechanics of its prosthesis, is better in this case. For persons who have undergone amputation, asymmetrical gait may be the instrument which protects the stump of the disabled limb. We can say that gait asymmetry would be a relevant measure for investigating the gait characteristics of amputees and establishing their propensity for future joint pain and degeneration (Nolan et al., 2003. For amputee gait, the choice of a suitable prosthesis is very important. The influence of a prosthetic foot on gait variables in a group of these subjects was observed by Gitter et al. (1991. Efficiency of performance of the gait cycle is also markedly influenced by a prosthesis or prosthetic foot alignment. Rehabilitation of an amputee can be considered to be successful only when the amputee finds the prosthesis aesthetic and comfortable during walking. Prosthesis alignment has to correspond to the demands of the prosthetist (Fridman, Ona, & Isakov, 2003. When the prosthetic foot is not aligned optimally, this fact can show up in different levels of gait cycle performance. During gait analysis, the prosthesis is intentionally "not optimally" aligned in order to elicit the implied implications. Fridman, Ona and Isakov (2003 observed the influence of a prosthetic foot positioned in external rotation. Schmalz, Blumentritt and Jarasch (2002 used sagittal shifting of the foot to both anterior and posterior directions and foot alignment to both plantar a dorsal flexion. Blumentritt et al. (1999 focussed on the effects of sagittal plane prosthetic alignment to the magnitude of knee joint loads on a standing transtibial amputee. The objectives of prosthetic alignment in persons with lower limb amputation are the enhancement of the remaining limb's comfort and to maximize the individual's walking capabilities. In biomechanical gait analysis, it is important to investigate not only the relationship between prosthetic foot alignment and the prosthetic limb, but also the relationship between this alignment and activity of the contralateral, sound limb (Pinzur et al., 1995. An accent on the sound limb appears in other studies (Nolan & Lees, 2000; Hurley et al., 1990, but there the question of various prosthetic alignments is not considered. This relationship between the sound and the prosthetic limb can be quantitative, measured by the symmetry or asymmetry of the measured variables.

  14. Vizuální kultura a současná média ve výtvarné výchově a vzdělávání u dětí předškolního věku, se zaměřením na reklamu

    OpenAIRE

    Sehnalová, Silvie

    2011-01-01

    This thesis deals with visual culture and media (with emphasis on advertisement) in art lessons of preschool children. One part is formed by a questionnaire in which preschool children and their parents reply to questions concerning their perception and influence of advertisement relating to contemporary media. The aim of the research is to get children's basic knowledge, attitudes and projections about advertisement and also parents' opinions on how their children are influenced by advertise...

  15. HCTISN - Plenary extraordinary meeting on the 9 March 2012 - General consequences of the earthquake and tsunami; Status of Fukushima-Dai-ichi nuclear installations; The Fukushima accident, one year after: environmental and health situation in Japan; Protective actions undertaken by Japanese authorities; Support by AREVA to Japan after the Fukushima accident; What went on in Fukushima? Implementation of the IAEA nuclear safety action plan; Review of European stress tests by the peers; Opinion of the ASN on complementary safety assessments (CSAs)

    International Nuclear Information System (INIS)

    Kataoka, Susumu; Charles, T.; Champion, Didier; Jean-Luc Godet; Arnaud GAY; Philippe Jamet

    2012-01-01

    This document contains Power Point presentations proposed during a plenary session of the High Committee transparency and information on nuclear safety (HCTISN). The contributions addressed the Fukushima accident (the earthquake and the tsunami, the technical consequences on the plant, the consequences on the environment and on health, the different actions undertaken in Japan to protect the population, the consequences on nuclear safety in other countries with notably the performance of stress tests or the organisation of complementary safety assessments on the French fleet of nuclear reactors

  16. Vzestupy a pády regionálního mocenského centra. Přehled současného stavu poznání pravěkého opevněného areálu na Vladaři v západních Čechách

    Czech Academy of Sciences Publication Activity Database

    Chytráček, Miloslav; Danielisová, Alžběta; Pokorný, P.; Kočár, Petr; Kyselý, René; Kyncl, T.; Sádlo, Jiří; Šmejda, L.; Zavřel, J.

    2012-01-01

    Roč. 103, prosinec (2012), s. 273-338 ISSN 0031-0506 R&D Projects: GA AV ČR IAA8002204 Grant - others:AV ČR(CZ) M300020903 Program:M Institutional research plan: CEZ:AV0Z80020508; CEZ:AV0Z60050516 Institutional support: RVO:67985912 ; RVO:67985939 Keywords : fortified settlement * power center * fortifications * Hallstatt period * La Tène period * cistern * radiocarbon dating * dendrochronological analysis * pollen analysis Subject RIV: AC - Archeology, Anthropology, Ethnology

  17. Impact of two common xeroderma pigmentosum group D (XPD gene polymorphisms on risk of prostate cancer.

    Directory of Open Access Journals (Sweden)

    Yuanyuan Mi

    Full Text Available BACKGROUND: DNA repair genes (EG: xeroderma pigmentosum group D, XPD may affect the capacity of encoded DNA repair enzymes to effectively remove DNA adducts or lesions, which may result in enhanced cancer risk. The association between XPD gene polymorphisms and the susceptibility of prostate cancer (PCa was inconsistent in previous studies. METHODOLOGY/PRINCIPAL FINDINGS: A meta-analysis based on 9 independent case-control studies involving 3165 PCa patients and 3539 healthy controls for XPD Gln751Lys SNP (single nucleotide polymorphism and 2555 cases and 3182 controls for Asn312Asp SNP was performed to address this association. Meanwhile, odds ratio (OR and 95% confidence intervals (CIs were used to evaluate this relationship. Statistical analysis was performed with STATA10.0. No significant association was found between XPD Gln751Lys SNP and PCa risk. On the other hand, in subgroup analysis based on ethnicity, associations were observed in Asian (eg. Asn vs. Asp: OR = 1.34, 95%CI = 1.16-1.55; Asn/Asn+Asn/Asp vs. Asp/Asp: OR = 1.23, 95%CI = 1.07-1.42 and African (eg. Asn vs. Asp: OR = 1.31, 95%CI = 1.01-1.70; Asn/Asn vs. Asp/Asp: OR = 1.71, 95%CI = 1.03-7.10 populations for Asn312Asp SNP. Moreover, similar associations were detected in hospital-based controls studies; the frequency of Asn/Asn genotype in early stage of PCa men was poorly higher than those in advanced stage of PCa men (OR = 1.45, 95%CI = 1.00-2.11. CONCLUSION/SIGNIFICANCE: Our investigations demonstrate that XPD Asn312Asp SNP not the Gln751Lys SNP, might poorly increase PCa risk in Asians and Africans, moreover, this SNPs may associate with the tumor stage of PCa. Further studies based on larger sample size and gene-environment interactions should be conducted to determine the role of XPD gene polymorphisms in PCa risk.

  18. New data on the Western Transylvanides along the Ampoi Valley (Southern Apuseni Mts., Romania

    Directory of Open Access Journals (Sweden)

    Erika Suciu-Krausz

    2006-04-01

    Full Text Available In order to clarify some of the issues regarding the mineralogical content and the source area of the Cretaceous deposits from Ampoi Valley basin (Southern Apuseni Mountains fourteen lithologic logs were drawn from the Ampoi Valley both side tributaries (Slatinii, Ruzi, Vâltori, Valea lui Paul, Feneş, Călineasa, Fierului Brook, Bobului, Satului, Tăuţi, Galaţi, Presaca Ampoiului, Valea Mică and Valea Mare brooks. The main sedimentary rock types were identified (conglomerates, wacke and lithic sandstones, clays, and marls. The sandstones were classified according to the ternary diagrams. Their petrographic features revealed both a magmatic and a metamorphic source area for them.

  19. Laser cutting of concretes with various ballasts

    International Nuclear Information System (INIS)

    Hamasaki, Masanobu; Katsumura, Munehide; Utsumi, Hiroaki

    1985-01-01

    The biological shield concrete and the radiation shield concrete which construct a part of the atomic reactor must be demolished with the decommissioning of the atomic reactor plants. In a case, the demolition using a laser is expected as one of excellent method for the decommissioning of these radioactive concretes. The fundamental cuttings of a mortar, the concretes with andesite, lime stone and gray wacke as ballast and a concrete reinforced with mild steel rods were therefore carried out using a 5 kW output CO 2 laser. As the results of experiment, it was cleared that cutting results varied with ballasts, 100 mm thick reinforced concrete could be cut, safety was high because few dross and few fume were produced. (author)

  20. Data of evolutionary structure change: 1LA6A-2ZLWD [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1LA6A-2ZLWD 1LA6 2ZLW A D -SLSDKDKAAVRALWSKIGKSSDAIGNDALSRMIVVYPQ...R VQLSGEEKAAVLALWDKV--NEEEVGGEALGRLLVVYPWTQRFFDSFGDLSNPGAVMGNPKVKAHGKKVLHSFGEGVHHLDNLKGTFAALSEL...line>ASN CA 245 2ZLW D 2ZLWD... 1 2ZLW D 2ZLWD LWD

  1. 76 FR 16609 - Proposed Information Collection; Comment Request; Identification of Human Cell Lines Project

    Science.gov (United States)

    2011-03-24

    ... differentiate among cell lines, as described in Designation: ASN-0002 Authentication of Human Cell Lines... NIST (contact information above). III. Data OMB Control Number: None. Form Number: None. Type of Review...

  2. Conserved water-mediated hydrogen bond network between TM-I, -II, -VI, and -VII in 7TM receptor activation

    DEFF Research Database (Denmark)

    Nygaard, Rie; Hansen, Louise Valentin; Mokrosinski, Jacek

    2010-01-01

    Five highly conserved polar residues connected by a number of structural water molecules together with two rotamer micro-switches, TrpVI:13 and TyrVII:20, constitute an extended hydrogen bond network between the intracellular segments of TM-I, -II, -VI, and -VII of 7TM receptors. Molecular dynamics...... to apparently function as a catching trap for water molecules. Mutational analysis of the beta2-adrenergic receptor demonstrated that the highly conserved polar residues of the hydrogen bond network were all important for receptor signaling but served different functions, some dampening constitutive activity...... (AsnI:18, AspII:10, and AsnVII:13), whereas others (AsnVII:12 and AsnVII:16) located one helical turn apart and sharing a water molecule were shown to be essential for agonist-induced signaling. It is concluded that the conserved water hydrogen bond network of 7TM receptors constitutes an extended...

  3. Regulatory framework and safety requirements for new (gen III) reactors

    International Nuclear Information System (INIS)

    Mourlon, Sophie

    2014-01-01

    Sophie Mourlon, ASN Deputy General Director, described the international process to enhance safety between local safety authorities through organizations such as WENRA. Then she explained to the participants the regulatory issues for the next generation of NPPs

  4. Production and enzyme engineerinq of human acetylcholinesterase and its mutant derivatives. Midterm report, 15 January 1993-15 July 1994

    Energy Technology Data Exchange (ETDEWEB)

    Shafferman, A.

    1994-07-15

    Specificity determinants of human acetylcholinesterase (HuAChE) towards ligands (substrate and some reversible and irreversible inhibitors) were identified by combination of site-directed mutagenesis, molecular modeling and kinetic studies with enzymes mutated in active center residues Trp86, Glu202, Trp286, Phe295, Phe297, Tyr337, Phe338 and Glu450. Thus, the anionic and hydrophobic subsites as well as the acyl pocket were identified. Enzymes with resistance to OP aging were engineered.The role of N-glycosylation in the function, biosynthesis and stability of HuAChE was examined by site-directed mutagenesis (Asn to GIn substitution) of the three potential N glycosylation sites, Asn265, Asn350 and Asn464. Large scale preparation of recombinant HuAChE was performed utilizing the microcarrier technology. Over 500 milligrams of enzyme was prepared for x-ray crystallography.

  5. ASN’s actions in GEN IV reactors and Sodium Fast Reactors (SFR)

    International Nuclear Information System (INIS)

    Belot, Clotilde

    2013-01-01

    The ASN is involved in 3 actions concerning GEN IV: • Overview of nuclear reactor GEN IV systems; • Specific analysis about transmutation; • Prototype reactor ASTRID (SFR). Furthermore theses actions are in the beginning (no conclusions or results available)

  6. Medical radio-physics. Quality assurance. Transparency; Radiophysique medicale. Assurance de la qualite. Transparence

    Energy Technology Data Exchange (ETDEWEB)

    Krembel, D.

    2009-07-15

    This short presentation, given by the national safety authority (ASN) at the meeting of July 1, 2009 of the high committee for the nuclear safety transparency and information (HCTISN), recalls, first, the missions of the ASN in the medical domain (radiation protection, control and inspections). Then it presents the reactions of the ASN in response to the increase of the number of radiological accidents since 2005: regulation reminder, circular letters, guidebooks, communication to the public and to the mass media. Finally, it defines the main priorities of the ASN with respect to radiotherapy: increase of human resources (radio-physicists shortage), declaration of incidents/accidents, quality assurance for the control of radiation doses delivered to patients, inspection of radiotherapy centres, expertise of new devices, improvement of softwares safety and ergonomics. (J.S.)

  7. Functional importance of PAI-1 glycosylation

    DEFF Research Database (Denmark)

    Christensen, Anni; Naessens, Dominik; Skottrup, Peter

    susceptible PAI-1 variant was not necessarily the one used when raising the antibody. This and other observations indicated that the carbohydrate moieties or the glycosylation sites are unlikely to be part of the epitopes for these antibodies. The antibody susceptibility characteristic for non......Structure-function studies of plasminogen activator inhibitor-1 (PAI-1) have previously been performed mostly with non-glycosylated material expressed in E. coli. We have now studied the importance of PAI-1 glycosylation for its functional properties. PAI-1 has 3 potential sites for N......-linked glycosylation. Biochemical analysis of PAI-1 variants with substitutions of the Asn residues in each of these sites and expression in human embryonic kidney 293 (HEK293) cells showed that only Asn211 and Asn 267, but not Asn331 are glycosylated, and revealed a differential composition of the carbohydrate...

  8. What are the external consulting needs?

    International Nuclear Information System (INIS)

    Limousin, S.; Crombez, S.; Monin, L.

    2010-01-01

    Obtaining and maintaining a high level of competence for designing, manufacturing and operating nuclear pressure equipment is the responsibility of manufacturers and licensees. However to make comprehensive and sound decisions a regulator needs independent expertise. ASN has just created a new 'standing committee' on nuclear pressure equipment. Added to that, ASN relies on his historical technical support IRSN, in particular on safety related topics or during reactor outages, when decisions need to be made in a short time. In the field of nuclear pressure equipment, it is often useful to compare practices with those in non nuclear industry. In such cases, ASN can contract with other technical support. For the conformity assessment of new equipment, French regulations states that ASN can delegate to accredited third party bodies part or the entire conformity assessment. (authors)

  9. Nuclear safety in France in 2001

    International Nuclear Information System (INIS)

    2002-01-01

    This press dossier summarizes the highlights of nuclear safety in France in 2001: the point-of-view of A.C. Lacoste, director of the French authority of nuclear safety (ASN), the new organisation of the control of nuclear safety and radiation protection, the ASN's policy of transparency, the evolutions of nuclear fuels and the consistency of the fuel cycle, the necessary evolutions of the nuclear crisis management, the harmonizing work of safety approaches carried out by the WENRA association. The following documents are attached in appendixes: the decrees relative to the reformation of the nuclear control in France, the missions of the ASN, the control of nuclear safety and radiation protection in France, the organization of ASN in March 2000, the incidents notified in 2001, the inspections performed in 2001, and the list of the main French nuclear sites. (J.S.)

  10. Nuclear safety in France in 2001; La surete nucleaire en France en 2001

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-07-01

    This press dossier summarizes the highlights of nuclear safety in France in 2001: the point-of-view of A.C. Lacoste, director of the French authority of nuclear safety (ASN), the new organisation of the control of nuclear safety and radiation protection, the ASN's policy of transparency, the evolutions of nuclear fuels and the consistency of the fuel cycle, the necessary evolutions of the nuclear crisis management, the harmonizing work of safety approaches carried out by the WENRA association. The following documents are attached in appendixes: the decrees relative to the reformation of the nuclear control in France, the missions of the ASN, the control of nuclear safety and radiation protection in France, the organization of ASN in March 2000, the incidents notified in 2001, the inspections performed in 2001, and the list of the main French nuclear sites. (J.S.)

  11. Change in amino acids content during germination and seedling ...

    African Journals Online (AJOL)

    presence of histidine (His) and phenylalanine (Phe), but also to amide amino acids asparagine (Asn), glutamine (Gln) and Arg contents. In Cola sp., free amino acids varied significantly during these two processes indicating their high utilization.

  12. Induction of Endoplasmic Reticulum Stress and Unfolded Protein Response Constitutes a Pathogenic Strategy of group A Streptococcus

    Directory of Open Access Journals (Sweden)

    Emanuel eHanski

    2014-08-01

    Full Text Available The connection between bacterial pathogens and unfolded protein response (UPR is poorly explored. In this review we highlight the evidence showing that group A streptococcus (GAS induces endoplasmic reticulum (ER stress and UPR through which it captures the amino acid asparagine (ASN from the host. GAS acts extracellularly and during adherence to host cells it delivers the hemolysin toxins; streptolysin O (SLO and streptolysin S (SLS. By poorly understood pathways, these toxins trigger UPR leading to the induction of the transcriptional regulator ATF4 and consequently to the upregulation of asparagine synthetase (ASNS transcription leading to production and release of ASN. GAS senses ASN and alters gene expression profile accordingly, and increases the rate of multiplication. We suggest that induction of UPR by GAS and by other bacterial pathogens represent means through which bacterial pathogens gain nutrients from the host, obviating the need to become internalized or inflict irreversible cell damage.

  13. HCTISN, plenary meeting of the 20 of June 2013

    International Nuclear Information System (INIS)

    2013-01-01

    This document gathers Power Point presentations which have been proposed during the meeting of the High committee for transparency and information on nuclear safety (HCTISN). A first PP, proposed by the ASN, presents the ASN's plan to implement the principle of public participation to the elaboration of its regulatory or individual decisions related to the environment. The second one, proposed by the ANDRA, presents the FAVL (low activity long life) waste storage project, notably for wastes containing radium, graphite or bitumen (waste classification, context, waste origin, temporary storage, long term management, project of small depth storage, site search process, project orientations). The third one, made by the ASN, discusses the opinion of the ASN on documents produced by the ANDRA since 2009 about the project of radioactive waste storage in deep geological layers (context, creation authorization procedure, general considerations, waste inventory, seismic data acquisition, direct storage of spent fuels coming from power reactors)

  14. Journal of Biosciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    bonding dynamics of catalytic Asn 175 in plant thiol protease ... Extensive energy minimization and molecular dynamics simulation studies up to 2 ns on all the PDB and solvated structures ... Water-mediated ionic interactions in protein structures.

  15. to control the nuclear safety and the radiation protection

    International Nuclear Information System (INIS)

    Lacoste, A.C.; Bordarier, Ph.; Saint-Raymond, Ph.; Repussard, J.; Gouze, J.R.; Degos, L.; Massart, S.; Wiroth, P.; Thezee, Ch.; Petit, G.; Cahen, B.; Hubert, I.; Wiroth, P.; Thezee, Ch.; Petit, G.; Kaufer, B.; Taniguchi, T.; Revol, H.

    2005-01-01

    Publishing this dossier, the aim is to present the principles and the variety of issues linked to nuclear safety and radiation protection supervision, and the main strategic choices made to use efficiently and effectively A.S.N. supervision means. A.S.N. is responsible for nuclear safety and radiation protection supervision. A.S.N. has to be itself evaluated and supervised by external bodies. The Parliament Office for Evaluation of Scientific and Technological Options (O.P.E.C.S.T.) supervises it; the foreign peers watch and A.S.N. has to be the object of an international audit conducted by its peers under the leadership of I.A.E.A. by the beginning of 2007. (N.C.)

  16. Medical radio-physics. Quality assurance. Transparency

    International Nuclear Information System (INIS)

    Krembel, D.

    2009-07-01

    This short presentation, given by the national safety authority (ASN) at the meeting of July 1, 2009 of the high committee for the nuclear safety transparency and information (HCTISN), recalls, first, the missions of the ASN in the medical domain (radiation protection, control and inspections). Then it presents the reactions of the ASN in response to the increase of the number of radiological accidents since 2005: regulation reminder, circular letters, guidebooks, communication to the public and to the mass media. Finally, it defines the main priorities of the ASN with respect to radiotherapy: increase of human resources (radio-physicists shortage), declaration of incidents/accidents, quality assurance for the control of radiation doses delivered to patients, inspection of radiotherapy centres, expertise of new devices, improvement of softwares safety and ergonomics. (J.S.)

  17. Un CERNOIS au sommet de la GTJ200 !!

    CERN Multimedia

    CERN Running Club

    2018-01-01

    Les 9 et 10 mars dernier a eu lieu la plus longue des courses de ski de fond au monde. C’est une épreuve qui cumule 200 km de ski de fond en empruntant les pistes de Giron (dans l’Ain) à Grand’Combe-Chateleu (dans le Doubs). Elle s’effectue par équipe de 2 avec l’aide d’une 3e personne pour l’assistance. Cette 4e édition a vu la victoire de Guillaume Michet et son binôme Herve Balanche. Ils sont allés chercher cette victoire grâce à une parfaite gestion de course et un magnifique esprit d’équipe (assistance comprise). Guillaume est un membre actif du running club. Ses entrainements assidus et intenses lui ont permis d’augmenter son niveau physique et de parfaire ses capacités mentales nécessaires pour arriver à bout d’une telle épreuve. Notons également...

  18. Nuclear safety authority. Strategical planning 2005- 2007

    International Nuclear Information System (INIS)

    Anon.

    2006-01-01

    The Nuclear safety Authority (A.S.N.) provides, in the name of the state, the monitoring of nuclear safety and radiation protection to protect workers, patients, public and environment from the risks in relation with nuclear activities and more broadly with ionizing radiations, it contributes to citizens information in these areas. The ambition of A.S.N. is to carry out a successful, legitimate, credible nuclear monitoring, recognized by citizens and that constitutes an international reference. (N.C.)

  19. Actions of the French Nuclear Safety Authority in response to the growth in public demand for environmental transparency

    International Nuclear Information System (INIS)

    Clipet, N.

    2010-01-01

    The Nuclear Transparency and Security Act 2006-686 of 13 June 2006 (TSN Act) reinforces the integrated approach to nuclear safety, radiation protection and environmental concerns. The TSN Act guarantees 'the public's right to reliable and accessible information on nuclear security' (Article 1). ASN fosters the implementation of this information right. Concerning the environment, it ensures that the available information is accessible and shared. This concern applies first to its own information media and actions, but also to the stakeholders. ASN makes sure that the licensees fulfill the transparency duty required by the TSN Act and is watchful concerning the effectiveness of means implemented for the public access to information. ASN also promotes good practices in its annual report for the public information. With regard to the environment, ASN actions are primarily focused on limiting and supervising the effluents discharges resulting from nuclear activities; monitoring radioactivity in the environment; and preventing and managing emergency situations. One of the recent significant contributions to providing the public with reliable, centralized information is the launch of www.mesure-radioactivite.fr. This web-site issued by the national network under the aegis of ASN, gives the public access to the results of radioactivity measurements carried out by certified laboratories. ASN seeks to involve the public in projects with an important impact on the environment. It considers the Local Information Committees (CLI) as an important contributor to the 'ecological democracy' and invites periodically their representatives to take part in ASN inspections. ASN also encourages public debates. Besides, this is one of the axis of its strategic plan 2010-2012. (author)

  20. Mitigation of the processing contaminant acrylamide in bread by reducing asparagine in the bread dough

    DEFF Research Database (Denmark)

    Katsaiti, Tatiana; Granby, Kit

    2016-01-01

    . Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for AAM and its main precursor, ASN, determination. The resulting ASN depletion in the dough (68–89%) is significantly affected by fermentation time and yeast type, while AAM mitigation levels in the baked buns are significantly...... influenced by yeast amount, fermentation time and yeast type. The mean concentrations for each combination range between 5 and 15 µg kg– 1....

  1. Relationship Between Genotype Variants Follicle-stimulating Hormone Receptor Gene Polymorphisms (FSHR) and Morphology of Oocytes Prior to ICSI Procedures

    OpenAIRE

    Gashi, Zafer; Elezaj, Shkelzen; Zeqiraj, Afrim; Grabanica, Driton; Shabani, Isak; Gruda, Bujar; Gashi, Fitore

    2016-01-01

    Introduction: This study investigated association of Asn680Ser FSHR polymorphism with the ovarian response in 104 women of Albanian ethnic population enrolled in ICSI program. The reason of infertility in all cases has been identified as male factor. Methods: Analysis of the Asn680Ser polymorphism was performed using TaqMan? SNP Genotyping Assay. Clinical and endocrinologic parameters were analyzed based on the genotype, age, BMI, oocyte yield, number of transferred embryos and pregnancy rate...

  2. Mechanistic understanding of N-glycosylation in Ebola virus glycoprotein maturation and function.

    Science.gov (United States)

    Wang, Bin; Wang, Yujie; Frabutt, Dylan A; Zhang, Xihe; Yao, Xiaoyu; Hu, Dan; Zhang, Zhuo; Liu, Chaonan; Zheng, Shimin; Xiang, Shi-Hua; Zheng, Yong-Hui

    2017-04-07

    The Ebola virus (EBOV) trimeric envelope glycoprotein (GP) precursors are cleaved into the receptor-binding GP 1 and the fusion-mediating GP 2 subunits and incorporated into virions to initiate infection. GP 1 and GP 2 form heterodimers that have 15 or two N -glycosylation sites (NGSs), respectively. Here we investigated the mechanism of how N -glycosylation contributes to GP expression, maturation, and function. As reported before, we found that, although GP 1 NGSs are not critical, the two GP 2 NGSs, Asn 563 and Asn 618 , are essential for GP function. Further analysis uncovered that Asn 563 and Asn 618 regulate GP processing, demannosylation, oligomerization, and conformation. Consequently, these two NGSs are required for GP incorporation into EBOV-like particles and HIV type 1 (HIV-1) pseudovirions and determine viral transduction efficiency. Using CRISPR/Cas9 technology, we knocked out the two classical endoplasmic reticulum chaperones calnexin (CNX) and/or calreticulin (CRT) and found that both CNX and CRT increase GP expression. Nevertheless, NGSs are not required for the GP interaction with CNX or CRT. Together, we conclude that, although Asn 563 and Asn 618 are not required for EBOV GP expression, they synergistically regulate its maturation, which determines its functionality. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  3. A haplotype of polymorphisms in ASE-1, RAI and ERCC1 and the effects of tobacco smoking and alcohol consumption on risk of colorectal cancer: a Danish prospective case-cohort study

    DEFF Research Database (Denmark)

    Hansen, Rikke D; Sørensen, Mette; Tjønneland, Anne

    2008-01-01

    BACKGROUND: Single nucleotide polymorphisms (SNPs) are the most frequent type of genetic variation in the human genome, and are of interest for the study of susceptibility to and protection from diseases. The haplotype at chromosome 19q13.2-3 encompassing the three SNPs ASE-1 G-21A, RAI IVS1 A4364G...... and ERCC1 Asn118Asn have been associated with risk of breast cancer and lung cancer. Haplotype carriers are defined as the homozygous carriers of RAI IVS1 A4364GA, ERCC1 Asn118AsnT and ASE-1 G-21AG. We aimed to evaluate whether the three polymorphisms and the haplotype are associated to risk of colorectal...... of colorectal cancer were found. No statistically significant interactions between the genotypes and the lifestyle exposures smoking or alcohol consumption were observed. CONCLUSION: Our results suggest that the ASE-1 G-21A, RAI IVS1 A4364G and ERCC1 Asn118Asn polymorphisms and the previously identified...

  4. Glycosylation analysis and protein structure determination of murine fetal antigen 1 (mFA1)--the circulating gene product of the delta-like protein (dlk), preadipocyte factor 1 (Pref-1) and stromal-cell-derived protein 1 (SCP-1) cDNAs

    DEFF Research Database (Denmark)

    Krogh, T N; Bachmann, E; Teisner, B

    1997-01-01

    By means of sequence analysis, murine fetal antigen 1 (mFA1) isolated from Mus musculus amniotic fluid was shown to be the circulating protein of the delta-like protein, stromal-cell-derived protein 1 (SCP-1) and preadipocyte factor 1 (Pref-1) gene products. The protein contains 36 cysteine...... residues arranged in six epidermal-growth-factor-like domains. The purification of several C-terminal peptides of varying lengths showed mFA1 to be C-terminal heterogeneous. O-linked glycosylations of the NeuNAc alpha2-3Gal beta1-3(NeuNAc alpha2-6)GalNAc type were present on all C-terminal peptides...... at residues Thr235, Thr244 and Thr248, although glycosylation on Thr244 was only partial. Three N-linked glycosylations were localized in mFA1 (Asn77, Asn142 and Asn151), two of which (Asn142 and Asn151) were in the unusual Asn-Xaa-Cys motif. Fucosylated biantennary complex-type and small amounts (less than 5...

  5. Adsorption of asparagine on the gold electrode and air/solution interface

    International Nuclear Information System (INIS)

    Slojkowska, R.; Palys, B.; Jurkiewicz-Herbich, M.

    2004-01-01

    The adsorption of asparagine (Asn) on a gold electrode from 0.1 M LiClO 4 aqueous solutions was investigated. The experimental data obtained from ac impedance measurements were analyzed to determine the dependence of adsorption parameters, i.e. the standard Gibbs energy of adsorption (ΔG 0 ), maximal value of surface excess concentration (Γ max ) of Asn and parameter of interactions in the adsorbed layer (A) on the electrode potential. The relatively large value of Gibbs energy of adsorption (∼ -47 kJ mol -1 ) gives the evidence of a very strong adsorption of Asn at the polycrystalline Au electrode. The comparison of the adsorption behavior of Asn at the air/solution and the Au/solution interfaces points out to the significant electronic interactions of adsorbate molecules with the Au electrode, since the adsorption of Asn on a free surface (from the same solutions) is very week. The analysis of the electrochemical data as well as the infrared reflection absorption spectroscopy (IRAS) results reveal that Asn molecules are anchored to the Au surface through oxygen atoms of the carboxylate group COO - and through the amide carbonyl group

  6. Labour inspection in nuclear power plant

    International Nuclear Information System (INIS)

    Vallet, J.; Polge, Ch.

    2009-01-01

    The French nuclear safety authority is in charge of labour inspection in nuclear power plants due to historical reasons. Thus, fifteen agents of ASN are acting simultaneously as nuclear inspectors and labour inspectors: they fulfill in the fifty eight reactor operated by EDF. ASN labour inspectors have the same rights ant duties than labour inspectors who fulfill in other French administrations. The regulatory domain is exactly the same. The work of ASN labour inspectors is divided in two main parts checking, on the one hand health and safety regulation requirements and on the other hand, social laws requirements. ASN labour inspectors assume, on their own, most of the decision they have to bring out. Nevertheless, ASN labour inspectors meet each other four times a year in order to share experiences. ASN labour inspectors and nuclear safety inspectors work together in the nuclear power plant. Its the principal benefit of this organisation. A high nu-clear safety level can not be reach without a good work conditions and social dialogue with labour unions. (author)

  7. Confirmation of emergence of mutations associated with atovaquone-proguanil resistance in unexposed Plasmodium falciparum isolates from Africa

    Directory of Open Access Journals (Sweden)

    Kyle Dennis E

    2006-10-01

    Full Text Available Abstract Background In vitro and in vivo resistance of Plasmodium falciparum to atovaquone or atovaquone-proguanil hydrochloride combination has been associated to two point mutations in the parasite cytochrome b (cytb gene (Tyr268Ser and Tyr268Asn. However, little is known about the prevalence of codon-268 mutations in natural populations of P. falciparum without previous exposure to the drug in Africa. Methods The prevalence of codon-268 mutations in the cytb gene of African P. falciparum isolates from Nigeria, Malawi and Senegal, where atovaquone-proguanil has not been introduced for treatment of malaria was assessed. Genotyping of the cytb gene in isolates of P. falciparum was performed by PCR-restriction fragment length polymorphism and confirmed by sequencing. Results 295 samples from Nigeria (111, Malawi (91 and Senegal (93 were successfully analyzed for detection of either mutant Tyr268Ser or Tyr268Asn. No case of Ser268 or Asn268 was detected in cytb gene of parasites from Malawi or Senegal. However, Asn268 was detected in five out of 111 (4.5% unexposed P. falciparum isolates from Nigeria. In addition, one out of these five mutant Asn268 isolates showed an additional cytb mutation leading to a Pro266Thr substitution inside the ubiquinone reduction site. Conclusion No Tyr268Ser mutation is found in cytb of P. falciparum isolates from Nigeria, Malawi or Senegal. This study reports for the first time cytb Tyr268Asn mutation in unexposed P. falciparum isolates from Nigeria. The emergence in Africa of P. falciparum isolates with cytb Tyr268Asn mutation is a matter of serious concern. Continuous monitoring of atovaquone-proguanil resistant P. falciparum in Africa is warranted for the rational use of this new antimalarial drug, especially in non-immune travelers.

  8. Confirmation of emergence of mutations associated with atovaquone-proguanil resistance in unexposed Plasmodium falciparum isolates from Africa.

    Science.gov (United States)

    Happi, Christian T; Gbotosho, Grace O; Folarin, Onikepe A; Milner, Danny; Sarr, Ousmane; Sowunmi, Akintunde; Kyle, Dennis E; Milhous, Wilbur K; Wirth, Dyann F; Oduola, Ayoade M J

    2006-10-04

    In vitro and in vivo resistance of Plasmodium falciparum to atovaquone or atovaquone-proguanil hydrochloride combination has been associated to two point mutations in the parasite cytochrome b (cytb) gene (Tyr268Ser and Tyr268Asn). However, little is known about the prevalence of codon-268 mutations in natural populations of P. falciparum without previous exposure to the drug in Africa. The prevalence of codon-268 mutations in the cytb gene of African P. falciparum isolates from Nigeria, Malawi and Senegal, where atovaquone-proguanil has not been introduced for treatment of malaria was assessed. Genotyping of the cytb gene in isolates of P. falciparum was performed by PCR-restriction fragment length polymorphism and confirmed by sequencing. 295 samples from Nigeria (111), Malawi (91) and Senegal (93) were successfully analyzed for detection of either mutant Tyr268Ser or Tyr268Asn. No case of Ser268 or Asn268 was detected in cytb gene of parasites from Malawi or Senegal. However, Asn268 was detected in five out of 111 (4.5%) unexposed P. falciparum isolates from Nigeria. In addition, one out of these five mutant Asn268 isolates showed an additional cytb mutation leading to a Pro266Thr substitution inside the ubiquinone reduction site. No Tyr268Ser mutation is found in cytb of P. falciparum isolates from Nigeria, Malawi or Senegal. This study reports for the first time cytb Tyr268Asn mutation in unexposed P. falciparum isolates from Nigeria. The emergence in Africa of P. falciparum isolates with cytb Tyr268Asn mutation is a matter of serious concern. Continuous monitoring of atovaquone-proguanil resistant P. falciparum in Africa is warranted for the rational use of this new antimalarial drug, especially in non-immune travelers.

  9. Optimization of Growth Conditions for Purification and Production of L-Asparaginase by Spirulina maxima

    Science.gov (United States)

    El Baroty, Gamal S.

    2016-01-01

    L-asparaginase (L-AsnA) is widely distributed among microorganisms and has important applications in medicine and in food technology sectors. Therefore, the ability of the production, purification, and characterization of AsnA from Spirulina maxima (SM) were tested. SM cultures grown in Zarrouk medium containing different N2 (in NaNO3 form) concentrations (1.25, 2.50, and 5.0 g/L) for 18 days contained a significant various quantity of dry biomass yields and AsnA enzyme levels. MS L-AsnA activity was found to be directly proportional to the N2 concentration. The cultures of SM at large scales (300 L medium, 5 g/L N2) showed a high AsnA enzyme activity (898 IU), total protein (405 mg/g), specific enzyme activity (2.21 IU/mg protein), and enzyme yield (51.28 IU/L) compared with those in low N2 cultures. The partial purification of crude MS AsnA enzyme achieved by 80% ammonium sulfate AS precipitated and CM-Sephadex C-200 gel filtration led to increases in the purification of enzyme with 5.28 and 10.91 times as great as that in SM crude enzymes. Optimum pH and temperature of purified AsnA for the hydrolyzate were 8.5 and 37 ± 0.2°C, respectively. To the best of our knowledge, this is the first report on L-asparaginase production in S. maxima. PMID:27525017

  10. Genetic and clinical predictors of ovarian response in assisted reproductive technology

    Science.gov (United States)

    Wiweko, B.; Damayanti, I.; Suryandari, D.; Natadisastra, M.; Pratama, G.; Sumapraja, K.; Meutia, K.; Iffanolia, P.; Harzief, A. K.; Hestiantoro, A.

    2017-08-01

    Several factors are known to influence ovarian response to rFSH stimulation such as age, antral follicle count (AFC), and basal FSH level, Mutation of allele Ser680Asn in FSHR gene was responsible to ovarian resistance toward exogenous FSH. The aim of this study is to develop a prediction model of ovarian response to COS in IVF. This study was a prospective cohort study. One hundred and thirteen women undergoing their first cycle of IVF in Yasmin IVF Clinic Jakarta were recruited to this study. Clinical datas included were age, BMI, and AFC. Basal FSH and E2 as well as serum AMH was measured from peripheral blood taken at second day of cycle. Bsr-1 enzyme is used to identify the polymorphism in exon 10 position 680 with RFLP technique. Three genotype polymorphism, Asn/Asn (255 bp ribbon), Asn/Ser (97 bp and 158 bp), and Ser/Ser (97 bp, 158 bp, and 255 bp). AFC has the highest predictor for ovarian response with AUC 0.922 (CI 95% 0.833-1.000). AMH also showed high predicting value (AUC 0.843 CI 95% 0.663-1.000). The multivariate analysis revealed combination of AFC, AMH, age, and basal FSH is a good model for ovarian response prediction (AUC=0.97). No significant relation between Asn/Asn, Asn/Ser, or Ser/Ser genotype FSHR polymorphism with ovarian response (p = 0.866) and total dose of rRSH (p = 0.08). This study showed that model combination of AFC, AMH, patient’s age and basal FSH are very good to predict number of mature oocytes.

  11. Optimization of Growth Conditions for Purification and Production of L-Asparaginase by Spirulina maxima

    Directory of Open Access Journals (Sweden)

    Hanaa H. Abd El Baky

    2016-01-01

    Full Text Available L-asparaginase (L-AsnA is widely distributed among microorganisms and has important applications in medicine and in food technology sectors. Therefore, the ability of the production, purification, and characterization of AsnA from Spirulina maxima (SM were tested. SM cultures grown in Zarrouk medium containing different N2 (in NaNO3 form concentrations (1.25, 2.50, and 5.0 g/L for 18 days contained a significant various quantity of dry biomass yields and AsnA enzyme levels. MS L-AsnA activity was found to be directly proportional to the N2 concentration. The cultures of SM at large scales (300 L medium, 5 g/L N2 showed a high AsnA enzyme activity (898 IU, total protein (405 mg/g, specific enzyme activity (2.21 IU/mg protein, and enzyme yield (51.28 IU/L compared with those in low N2 cultures. The partial purification of crude MS AsnA enzyme achieved by 80% ammonium sulfate AS precipitated and CM-Sephadex C-200 gel filtration led to increases in the purification of enzyme with 5.28 and 10.91 times as great as that in SM crude enzymes. Optimum pH and temperature of purified AsnA for the hydrolyzate were 8.5 and 37 ± 0.2°C, respectively. To the best of our knowledge, this is the first report on L-asparaginase production in S. maxima.

  12. The C-terminal N-glycosylation sites of the human alpha1,3/4-fucosyltransferase III, -V, and -VI (hFucTIII, -V, adn -VI) are necessary for the expression of full enzyme activity.

    Science.gov (United States)

    Christensen, L L; Jensen, U B; Bross, P; Orntoft, T F

    2000-09-01

    The alpha1,3/4-fucosyltransferases are involved in the synthesis of fucosylated cell surface glycoconjugates. Human alpha1,3/4-fucosyltransferase III, -V, and -VI (hFucTIII, -V, and -VI) contain two conserved C-terminal N-glycosylation sites (hFucTIII: Asn154 and Asn185; hFucTV: Asn167 and Asn198; and hFucTVI: Asn153 and Asn184). In the present study, we have analyzed the functional role of these potential N-glycosylation sites, laying the main emphasis on the sites in hFucTIII. Tunicamycin treatment completely abolished hFucTIII enzyme activity while castanospermine treatment diminished hFucTIII enzyme activity to approximately 40% of the activity of the native enzyme. To further analyze the role of the conserved N-glycosylation sites in hFucTIII, -V, and -VI, we made a series of mutant genomic DNAs in which the asparagine residues in the potential C-terminal N-glycosylation sites were replaced by glutamine. Subsequently, the hFucTIII, -V, and -VI wild type and the mutants were expressed in COS-7 cells. All the mutants exhibited lower enzyme activity than the wild type and elimination of individual sites had different effects on the activity. The mutations did not affect the protein level of the mutants in the cells, but reduced the molecular mass as predicted. Kinetic analysis of hFucTIII revealed that lack of glycosylation at Asn185 did not change the Km values for the oligosaccharide acceptor and the nucleotide sugar donor. The present study demonstrates that hFucTIII, -V, and -VI require N-glycosylation at the two conserved C-terminal N-glycosylation sites for expression of full enzyme activity.

  13. Hybrid functional band gap calculation of SnO{sub 6} containing perovskites and their derived structures

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Hyewon [Department of Physics, Pusan National University, Pusan 609-735, Republic of South Korea (Korea, Republic of); Cheong, S.W. [Rutgers Center for Emergent Materials and Department of Physics and Astronomy, Rutgers University, Piscataway, NJ 08854 (United States); Kim, Bog G., E-mail: boggikim@pusan.ac.kr [Department of Physics, Pusan National University, Pusan 609-735, Republic of South Korea (Korea, Republic of)

    2015-08-15

    We have studied the properties of SnO{sub 6} octahedra-containing perovskites and their derived structures using ab initio calculations with different density functionals. In order to predict the correct band gap of the materials, we have used B3LYP hybrid density functional, and the results of B3LYP were compared with those obtained using the local density approximation and generalized gradient approximation data. The calculations have been conducted for the orthorhombic ground state of the SnO{sub 6} containing perovskites. We also have expended the hybrid density functional calculation to the ASnO{sub 3}/A'SnO{sub 3} system with different cation orderings. We propose an empirical relationship between the tolerance factor and the band gap of SnO{sub 6} containing oxide materials based on first principles calculation. - Graphical abstract: (a) Structure of ASnO{sub 3} for orthorhombic ground state. The green ball is A (Ba, Sr, Ca) cation and the small (red) ball on edge is oxygen. SnO{sub 6} octahedrons are plotted as polyhedron. (b) Band gap of ASnO{sub 3} as a function of the tolerance factor for different density functionals. The experimental values of the band gap are marked as green pentagons. (c) ASnO{sub 3}/A'SnO{sub 3} superlattices with two types cation arrangement: [001] layered structure and [111] rocksalt structure, respectively. (d) B3LYP hybrid functional band gaps of ASnO{sub 3}, [001] ordered superlattices, and [111] ordered superlattices of ASnO{sub 3}/A'SnO{sub 3} as a function of the effective tolerance factor. Note the empirical linear relationship between the band gap and effective tolerance factor. - Highlights: • We report the hybrid functional band gap calculation of ASnO{sub 3} and ASnO{sub 3}/A'SnO{sub 3}. • The band gap of ASnO{sub 3} using B3LYP functional reproduces the experimental value. • We propose the linear relationship between the tolerance factor and the band gap.

  14. Hybrid functional band gap calculation of SnO6 containing perovskites and their derived structures

    International Nuclear Information System (INIS)

    Lee, Hyewon; Cheong, S.W.; Kim, Bog G.

    2015-01-01

    We have studied the properties of SnO 6 octahedra-containing perovskites and their derived structures using ab initio calculations with different density functionals. In order to predict the correct band gap of the materials, we have used B3LYP hybrid density functional, and the results of B3LYP were compared with those obtained using the local density approximation and generalized gradient approximation data. The calculations have been conducted for the orthorhombic ground state of the SnO 6 containing perovskites. We also have expended the hybrid density functional calculation to the ASnO 3 /A'SnO 3 system with different cation orderings. We propose an empirical relationship between the tolerance factor and the band gap of SnO 6 containing oxide materials based on first principles calculation. - Graphical abstract: (a) Structure of ASnO 3 for orthorhombic ground state. The green ball is A (Ba, Sr, Ca) cation and the small (red) ball on edge is oxygen. SnO 6 octahedrons are plotted as polyhedron. (b) Band gap of ASnO 3 as a function of the tolerance factor for different density functionals. The experimental values of the band gap are marked as green pentagons. (c) ASnO 3 /A'SnO 3 superlattices with two types cation arrangement: [001] layered structure and [111] rocksalt structure, respectively. (d) B3LYP hybrid functional band gaps of ASnO 3 , [001] ordered superlattices, and [111] ordered superlattices of ASnO 3 /A'SnO 3 as a function of the effective tolerance factor. Note the empirical linear relationship between the band gap and effective tolerance factor. - Highlights: • We report the hybrid functional band gap calculation of ASnO 3 and ASnO 3 /A'SnO 3 . • The band gap of ASnO 3 using B3LYP functional reproduces the experimental value. • We propose the linear relationship between the tolerance factor and the band gap

  15. Mechanistic and structural basis of stereospecific Cbeta-hydroxylation in calcium-dependent antibiotic, a daptomycin-type lipopeptide.

    Science.gov (United States)

    Strieker, Matthias; Kopp, Florian; Mahlert, Christoph; Essen, Lars-Oliver; Marahiel, Mohamed A

    2007-03-20

    Non-ribosomally synthesized lipopeptide antibiotics of the daptomycin type are known to contain unnatural beta-modified amino acids, which are essential for bioactivity. Here we present the biochemical and structural basis for the incorporation of 3-hydroxyasparagine at position 9 in the 11-residue acidic lipopeptide lactone calcium-dependent antibiotic (CDA). Direct hydroxylation of l-asparagine by AsnO, a non-heme Fe(2+)/alpha-ketoglutarate-dependent oxygenase encoded by the CDA biosynthesis gene cluster, was validated by Fmoc derivatization of the reaction product and LC/MS analysis. The 1.45, 1.92, and 1.66 A crystal structures of AsnO as apoprotein, Fe(2+) complex, and product complex, respectively, with (2S,3S)-3-hydroxyasparagine and succinate revealed the stereoselectivity and substrate specificity of AsnO. The comparison of native and product-complex structures of AsnO showed a lid-like region (residues F208-E223) that seals the active site upon substrate binding and shields it from sterically demanding peptide substrates. Accordingly, beta-hydroxylated asparagine is synthesized prior to its incorporation into the growing CDA peptide. The AsnO structure could serve as a template for engineering novel enzymes for the synthesis of beta-hydroxylated amino acids.

  16. Nuclear Safety and Radiation Protection in France in 2005

    International Nuclear Information System (INIS)

    2006-04-01

    In 2005, the Asn pursued its significant investment in radiation protection and reaffirms its ambition to become as efficient in radiation protection as it is in nuclear safety as of 2009. 2005 was a year of great progress for the Asn as it consolidated its organisation and working methods, in accordance with the 2005-2007 strategic plan it set for itself. The Asn continued progress in the field of radiation protection has given rise to various new regulations to improve the legislative and regulatory framework in this area. The Asn plans to step up its efforts to ensure better monitoring of patient exposure to ionizing radiation and to provide better management of radon-related risks, particularly in housing. Fully aware that its newfound power in this area requires outside evaluation, the Asn has asked the International Atomic Energy Agency (IAEA) to organize an I.R.R.S. (Integrated Regulatory Review Service) assignment consisting of a peer-conducted audit. The IAEA has confirmed that this audit will take place in November 2006. (N.C.)

  17. The control of nuclear sector; Le controle du nucleaire

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    The Asn is loaded with the control of the nuclear safety and the radiation protection in France: it provides this control, in the name of the state, to protect the workers, the patients, the public and the environment of the risks in relation with nuclear activities. The control is the core business of Asn. Asn so checks the nuclear basic installations (I.N.B.), since their conception until their dismantling, the pressure equipment specially conceived for these installations, the management of the radioactive waste as well as the transport of radioactive substances. Asn also checks all the industrial and research installations as well as the hospitals where are used ionizing radiations. It is a more recent profession there, because dating the reform of the control of the nuclear power of 2002, which constitutes that of the radiation protection. The first responsibility of the activities at risks falls to the one who begins them. This principle applies to all the sectors checked by Asn: an industrialist is responsible for the safety of the nuclear installations which he exploits, a doctor is responsible for the use of the ionizing radiations which he uses. (N.C.)

  18. Viologen-Phosphorus Dendrimers Inhibit α-Synuclein Fibrillation.

    Science.gov (United States)

    Milowska, Katarzyna; Grochowina, Justyna; Katir, Nadia; El Kadib, Abdelkrim; Majoral, Jean-Pierre; Bryszewska, Maria; Gabryelak, Teresa

    2013-03-04

    Inhibition of α-synuclein (ASN) fibril formation is a potential therapeutic strategy in Parkinson's disease and other synucleinopathies. The aim of this study was to examine the role of viologen-phosphorus dendrimers in the α-synuclein fibrillation process and to assess the structural changes in α-synuclein under the influence of dendrimers. ASN interactions with phosphonate and pegylated surface-reactive viologen-phosphorus dendrimers were examined by measuring the zeta potential, which allowed determining the number of dendrimer molecules that bind to the ASN molecule. The fibrillation kinetics and the structural changes were examined using ThT fluorescence and CD spectroscopy. Depending on the concentration of the used dendrimer and the nature of the reactive groups located on the surface, ASN fibrillation kinetics can be significantly reduced, and even, in the specific case of phosphonate dendrimers, the fibrillation can be totally inhibited at low concentrations. The presented results indicate that viologen-phosphorus dendrimers are able to inhibit ASN fibril formation and may be used as fibrillar regulating agents in neurodegenerative disorders.

  19. Nuclear Safety and Radiation Protection in France in 2005; La surete nucleaire et la radioprotection en France en 2005. Dossier de presse

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-04-15

    In 2005, the Asn pursued its significant investment in radiation protection and reaffirms its ambition to become as efficient in radiation protection as it is in nuclear safety as of 2009. 2005 was a year of great progress for the Asn as it consolidated its organisation and working methods, in accordance with the 2005-2007 strategic plan it set for itself. The Asn continued progress in the field of radiation protection has given rise to various new regulations to improve the legislative and regulatory framework in this area. The Asn plans to step up its efforts to ensure better monitoring of patient exposure to ionizing radiation and to provide better management of radon-related risks, particularly in housing. Fully aware that its newfound power in this area requires outside evaluation, the Asn has asked the International Atomic Energy Agency (IAEA) to organize an I.R.R.S. (Integrated Regulatory Review Service) assignment consisting of a peer-conducted audit. The IAEA has confirmed that this audit will take place in November 2006. (N.C.)

  20. Prion protein gene analysis in three kindreds with fatal familial insomnia (FFI): Codon 178 mutation and codon 129 polymorphism

    Energy Technology Data Exchange (ETDEWEB)

    Medori, R.; Tritschler, H.J. (Universita di Bologna (Italy))

    1993-10-01

    Fatal familial insomnia (FFI) is a disease linked to a GAC(Asp) [yields] AAC(Asn) mutation in codon 178 of the prion protein (PrP) gene. FFI is characterized clinically by untreatable progressive insomnia, dysautonomia, and motor dysfunctions and is characterized pathologically by selective thalamic atrophy. The authors confirmed the 178[sup Asn] mutation in the PrP gene of a third FFI family of French ancestry. Three family members who are under 40 years of age and who inherited the mutation showed only reduced perfusion in the basal ganglia on single photon emission computerized tomography. Some FFI features differ from the clinical and neuropathologic findings associated with 178[sup Asn] reported elsewhere. However, additional intragenic mutations accounting for the phenotypic differences were not observed in two affected individuals. In other sporadic and familial forms of Creutzfeldt-Jakob disease and Gerstmann-Straeussler syndrome, Met or Val homozygosity at polymorphic codon 129 is associated with a more severe phenotype, younger age at onset, and faster progression. In FFI, young and old individuals at disease onset had 129[sup Met/Val]. Moreover, of five 178[sup Asn] individuals who are above age-at-onset range and who are well, two have 129[sup Met] and three have 129[sup Met/Val], suggesting that polymorphic site 129 does not modulate FFI phenotypic expression. Genetic heterogeneity and environment may play an important role in inter- and intrafamilial variability of the 178[sup Asn] mutation. 32 refs., 5 figs., 1 tab.

  1. Silencing of DNase Colicin E8 Gene Expression by a Complex Nucleoprotein Assembly Ensures Timely Colicin Induction.

    Directory of Open Access Journals (Sweden)

    Simona Kamenšek

    2015-06-01

    Full Text Available Colicins are plasmid-encoded narrow spectrum antibiotics that are synthesized by strains of Escherichia coli and govern intraspecies competition. In a previous report, we demonstrated that the global transcriptional factor IscR, co dependently with the master regulator of the DNA damage response, LexA, delays induction of the pore forming colicin genes after SOS induction. Here we show that IscR is not involved in the regulation of nuclease colicins, but that the AsnC protein is. We report that AsnC, in concert with LexA, is the key controller of the temporal induction of the DNA degrading colicin E8 gene (cea8, after DNA damage. We demonstrate that a large AsnC nucleosome-like structure, in conjunction with two LexA molecules, prevent cea8 transcription initiation and that AsnC binding activity is directly modulated by L asparagine. We show that L-asparagine is an environmental factor that has a marked impact on cea8 promoter regulation. Our results show that AsnC also modulates the expression of several other DNase and RNase colicin genes but does not substantially affect pore-forming colicin K gene expression. We propose that selection pressure has "chosen" highly conserved regulators to control colicin expression in E. coli strains, enabling similar colicin gene silencing among bacteria upon exchange of colicinogenic plasmids.

  2. Face-selective crystal growth behavior of L-aspartic acid in the presence of L-asparagine

    Science.gov (United States)

    Sato, Hiroyasu; Doki, Norihito; Yoshida, Saki; Yokota, Masaaki; Shimizu, Kenji

    2016-02-01

    The kinetic mechanism of L-asparagine (L-Asn) action on L-aspartic acid (L-Asp) crystal growth, namely the face-selective effect of L-Asn on the L-Asp crystal growth rate in each direction, was examined. In the a-axis direction, the effect of L-Asn on the L-Asp crystal growth rate was small. Enhancement and inhibition of L-Asp crystal growth, and interestingly the dissolution of the L-Asp crystal face, were observed in the b-axis direction, depending on the amount of L-Asn added. In the c-axis direction, the L-Asp crystal growth rate decreased with the increase in the amount of L-Asn added, and the experimental results were well fitted with a Langmuir adsorption isotherm. The study showed that there were crystal growth conditions where enhancement and inhibition, as well as inhibition and dissolution, coexisted in the presence of an additive with a structure similar to the growing crystal.

  3. The control of nuclear sector

    International Nuclear Information System (INIS)

    2007-01-01

    The Asn is loaded with the control of the nuclear safety and the radiation protection in France: it provides this control, in the name of the state, to protect the workers, the patients, the public and the environment of the risks in relation with nuclear activities. The control is the core business of Asn. Asn so checks the nuclear basic installations (I.N.B.), since their conception until their dismantling, the pressure equipment specially conceived for these installations, the management of the radioactive waste as well as the transport of radioactive substances. Asn also checks all the industrial and research installations as well as the hospitals where are used ionizing radiations. It is a more recent profession there, because dating the reform of the control of the nuclear power of 2002, which constitutes that of the radiation protection. The first responsibility of the activities at risks falls to the one who begins them. This principle applies to all the sectors checked by Asn: an industrialist is responsible for the safety of the nuclear installations which he exploits, a doctor is responsible for the use of the ionizing radiations which he uses. (N.C.)

  4. The concept of time in early twentieth-century philosophy a philosophical thematic atlas

    CERN Document Server

    2016-01-01

    This book presents a collection of authoritative contributions on the concept of time in early twentieth-century philosophy. It is structured in the form of a thematic atlas: each section is accompanied by relevant elementary logic maps that reproduce in a “spatial” form the directionalities (arguments and/or discourses) reported on in the text. The book is divided into three main sections, the first of which covers phenomenology and the perception of time by analyzing the works of Bergson, Husserl, Sartre, Merleau-Ponty, Deleuze, Guattari and Derrida. The second section focuses on the language and conceptualization of time, examining the works of Cassirer, Wittgenstein, Heidegger, Lacan, Ricoeur and Foucault, while the last section addresses the science and logic of time as they appear in the works of Guillaume, Einstein, Reichenbach, Prigogine and Barbour. The purpose of the book is threefold: to provide readers with a comprehensive overview of the concept of time in early twentieth-century philosophy; ...

  5. A phenomenological attempt at understanding otherness

    Directory of Open Access Journals (Sweden)

    A. KOVÁCS

    2017-12-01

    Full Text Available The phenomenology of otherness is not satisfied with the reductionist definitions of the classical anthropological conceptions. The latter have identified the essence of man in his rationality, morality, createdness, or the possibility of moral and aesthetic self-perfection. The monolithic definition of human essence, based on uniform criteria, seems today one-sided and outdated. The parallel effects of cultural diversification, the pluralized political and social system, and multilingualism have directly and inevitably confronted us with otherness and strangeness. We could even say that we can understand our identity primarily through the experience of otherness. We will reach our conclusions related to the phenomenological constitutive of otherness by way of the interpretation of the relevant ideas of Baudrillad, Guillaume and Lévinas.

  6. Commented Portuguese translation of Guillaume’s paper of 1896 about the temperature of space

    Directory of Open Access Journals (Sweden)

    A K. T. Assis

    2014-11-01

    Full Text Available We present a complete Portuguese translation of the 1896 paper by Charles Édouard Guillaume (1861-1928 about the temperature of interstellar space. The importance of this work is that it is the oldest estimate known to us of the temperature acquired by a body which is in interstellar space far away from other stars. This temperature would be due to an equilibrium state in which the radiation received by this body from the stars around it would be equal to the radiation emitted by this body. This estimative happened prior to the birth of George Gamow (1904-1968 who is considered erroneously in many textbooks as being the first person to have predicted this temperature.

  7. The description and symbolic value of the unicorn in French literature of the 12th and 13th centuries

    Directory of Open Access Journals (Sweden)

    Marija Panić

    2016-02-01

    Full Text Available The paper considers naturalist and symbolic traits attributed to the unicorn in French 12th and 13th century literature. The corpus encompasses the bestiaries comprised by Philippe de Thaon, Gervaise, Guillaume de Normandy, Pierre de Beauvais, Richard de Fournival and pseudo-Pierre de Beauveis, which belong to the tradition of the Physiologoi, as well as other encyclopedic and other works which contain the bookish zoological and geographic knowledge of the middle ages: Mappemonde by Pierre de Beauvais, The letter of presbyter John, and the Mappemonde by Gossouin de Metz. In this corpus where the description of the animals comes mainly from the same written sources (from antiquity and late antiquity and the Bible, we consider how this nonexistent animal is described and to what extent a symbolic interpretation is present.

  8. Newton da Costa and the school of Curitiba

    Directory of Open Access Journals (Sweden)

    Artibano Micali

    2011-06-01

    Full Text Available This paper intends to report on the beginning of the publications of Newton da Costa outside Brazil. Two mathematicians played an important role in this beginning: Marcel Guillaume from the University of Clermont-Ferrand and Paul Dedecker from the Universities of Lille and Liège. At the same time we recall the role played by Newton da Costa and Jayme Machado Cardoso in the development of what we call here the School of Curitiba [Escola de Curitiba]. Paraconsistent logic was initiated in this school under the influence of Newton da Costa. As another contribution of this school we mention the development of the theory of quasigroups; Jayme Machado Cardoso's name has been given, by Sade, to some particular objects which are now called Cardoso quasigroups.

  9. Realisme, teatralitet, ritual

    DEFF Research Database (Denmark)

    Petersen, Anne Ring

    2008-01-01

    , and that they are characterised by a heightened awareness as to the physical, subjective and temporal aspects of the viewer's experience.  Moreover, it argues that the elements mentioned in the title "Realism, theatricality, ritual" are key characteristics of this genre. It demonstrates this by using a range of different works......The ambition of this article is to define what characterises installation art in general: It proposes that basically installations organise the work of art as a space and activate this space, that they extend the work temporally so that it acquires a situational aspect...... by Mike Kelley, Guillaume Bijl, Anette Messager, Carsten Höller and other artists as examples. The book is published in two editions, one in Danish and one in English....

  10. Realism, theatricality, ritual

    DEFF Research Database (Denmark)

    Petersen, Anne Ring

    2008-01-01

    , and that they are characterised by a heightened awareness as to the physical, subjective and temporal aspects of the viewer's experience.  Moreover, it argues that the elements mentioned in the title "Realism, theatricality, ritual" are key characteristics of this genre. It demonstrates this by using a range of different works......The ambition of this article is to define what characterises installation art in general: It proposes that basically installations organise the work of art as a space and activate this space, that they extend the work temporally so that it acquires a situational aspect...... by Mike Kelley, Guillaume Bijl, Anette Messager, Carsten Höller and other artists as examples. The book is published in two editions, one in English and one in Danish....

  11. Le don en anamorphose ou la réécriture du monde. Configurations et enjeux de l’hospitalité dans le récit médiéval (XIIe-XIIIe siècles

    Directory of Open Access Journals (Sweden)

    Carlos Fonseca Clamote Carreto

    2009-05-01

    Full Text Available  « A la limite, le don comme don devrait ne pas apparaître comme don ni au donataire, ni au donateur […]. En ce sens, le don est l’impossible. Non pas impossible, mais la figure même de l’impossible. » J. Derrida, Donner le temps.« Biaz sire, fet il, bien vaingniez.Se o moi herbergiez daingniez,Vez l’ostel aparelliés »Chrétien de Troyes, Érec et Énide.Arthur ou la largesse infinieÀ une époque où, de Jean de Salisbury à Brunetto Latini, en passant par Jacques de Vitry ou Guillaume Le Clerc de ...

  12. Gavarni at the casino: reflections of class and gender in the visual culture of 1848.

    Science.gov (United States)

    Briggs, Jo

    2011-01-01

    Drawing on cultural history and opposing the idea that British visual culture in 1848 has nothing interesting to say about class, politics, and revolution, this article examines a depiction of the London dance-hall Laurent's Casino by Guillaume-Sulpice Chevalier, better known as Paul Gavarni, made to accompany as essay by Albert Smith for the collaborative work "Gavarni in London" (1849). The image appeared against the backdrop of revolutions abroad, and just weeks after rioting in Trafalgar Square. Reconstructing the location of the casino, the history of the venue, the entertainments enjoyed there, and description of its patrons - the gent and his female counterpart - reveals the dynamic performance of class enacted there, and contemporary reactions to that performance.

  13. Aznar, Hanotin, and May (eds., À la place du roi. Vice-rois, gouverneurs et ambassadeurs dans les monarchies française et espanole (Casa de Velázquez, 2014

    Directory of Open Access Journals (Sweden)

    Lisandra Estevez

    2016-06-01

    Full Text Available The French and Spanish courts were two of the most powerful cultural and political institutions in seventeenth-century Europe.  Both rivals and allies, the French and Spanish crowns were bound by disputes and diplomacy.  This anthology examines the Gallic and Iberian court systems from a comparative approach, filling an important lacuna in early modern political studies and diplomatic history.  It is based on the proceedings of a seminar held at the Casa de Velázquez, Madrid in June 2010. Edited by Daniel Aznar, Guillaume Hanotin and Niels F. May, À la place du roi. Vice-rois, gouverneurs et ambassadeurs dans les monarchies française et espanole (XVIe-XVIIIe siècles unites ten essays by leading scholars of seventeenth-century French and Spanish history.

  14. Liminaire

    OpenAIRE

    Husson, Laurent; Seydoux, Guillaume

    2008-01-01

    Le Portique ne pouvait rester indifférent au centenaire de la nais­sance de Sartre. Six philosophes des deux côtés du Rhin, Manfred Frank (Tübingen), Guillaume Seydoux (Metz), Jean-Christophe Merle (Tours et Saarbrücken), Laurent Husson (Metz), Alain Flajo­liet (Paris) et Gerhard Seel (Berne) ont interrogé sa pensée philoso­phique à partir de ses fonde­ments ontologiques et de ses perspectives morales. La théorie sartrienne de la conscience, éclairée dans sa singula­rité à l’aide des traditi...

  15. Members of the Forum Engelberg visit CERN

    CERN Multimedia

    Patrice Loïez

    2002-01-01

    The Forum Engelberg is an annual interdisciplinary conference held in Engelberg, Switzerland intended to act as an international platform for debate and exchange of views on key issues affecting scientific research, technology, economics and philosophy. Its President is Hubert Curien - former French Minister of Research and Space Research, and President of the CERN Council from 1994 to 1996. Photo 01: (left to right) Konrade Von Bremen, Claude-Olivier Rochat, T. Virdee and Guillaume Pictet in front of one of the two end-caps of the CMS detector at the assembly site. The brass structure in the top right-hand corner is part of the hadronic calorimeter that will measure the energy of strongly interacting particles produced in collisions at the LHC.

  16. Exploration for sandstone- type uranium mineralisation in the Siwaliks of northwestern Himalaya, India

    International Nuclear Information System (INIS)

    Swarnkar, B.M.; Kothari, P.K.; Umamaheswar, K.; Srinivasan, S.

    2002-01-01

    The Siwalik Group with a thickness of about 6000m of fluvial sediments of middle Miocene to Pleistocene age has been explored extensively over two decades for U, using various types of exploration techniques involving air-borne gamma-ray spectrometry, radiation jeep survey, hydrogeochemical survey, ground radiometric survey, radon survey, exploratory drilling and mining, Exploration effort by the Atomic Minerals Directorate for Exploration and Research (AMD) has helped in identifying numerous uranium occurrences spread over the entire Siwalik belt between Poonch (Jammu and Kashmir) in the west and Tanakpur (Uttar Pradesh) in the east, in the northwest Himalaya. Eight significant zones were delineated, mostly confining to distinct stratigraphic horizons of the transition zone between Middle and Upper Siwaliks, and occasionally the transition zone between Lower and Middle Siwaliks. These mineralised zones have a considerable lateral extent of up to 12 km and are associated with sandstones and rarely conglomerates. Uranium mineralisation occurs in the form of peneconcordant lensoidal bodies with individual lenses traceable from a few tens of metres to 700m, sub-parallel to strike or dip, with average grades varying from 0.020 - 0.060% U 3 O 8 and thickness less than a metre to 4m. The host rock of uranium mineralisation is predominantly sandstone containing carbonaceous matter, pyrite and clay pellets. The sandstone is often arkosic and micaceous, and termed as lithic wacke and arkosic wacke. The uranium minerals present are uraninite, pitchblende, coffinite and secondary minerals such as tyuyamunite, metatyuyamunite, uranophane, bayleyite, andersonite, schoepite, liebegite, swartzite, schroekingerite, wulfenite, billictite, betauranophane, autunite and torbernite. Relatively higher concentrations or Se, Mo, Cu, Co, V and Au have been noted in a few uranirerous zones. Concentration or uranium in the Siwalik clastic sediments is controlled by the redox interface

  17. Amerigo di Narbona, ultimo sovrano di Arborea?

    Directory of Open Access Journals (Sweden)

    Gallinari, Luciano

    1999-12-01

    Full Text Available In the light of a new document that has been found in the Archivo de la Corona de Aragón in Barcelona, this article presents a new outline of the political situation in the interior of the "Giudicato" or Kingdom of Arborea while the news concerning the agreement between the "judge" of Arborea William II, viscount of Narbonne, and the king of Aragon, Ferdinand I, for the selling of the "judge" 's rights about the "Giudicato" spread in Sassari in 1414. In consequence of this agreement, Sassari, capital town of Arborea, refusing the William II's decision, elected new "judge" of Arborea Aymeric of Narbonne, viscount's brother, and took the oath.[fr] A la lumière d'un nouveau document des Archives de la Couronne d'Aragon de Barcelona, cet article présente des nouveaux données sur la situation politique à l'intérieur du Judicat ou royaume d'Arborée lorsque en 1414 à Sassari se répandit la nouvelle que le juge d'Arborea Guillaume II, vicomte de Narbonne, et le roi d'Aragon Ferdinan 1er étaient arrivés à un accord pour la vente des droits que le juge avait sur le Judical sarde. En consequence de cet accori, la capitale judicale Sassari, en refusant la décision de Guillaume II, nomma nouveau juge d'Arborée Aymeric de Narbonne frère du vicomte, en lui prêtant serment.

  18. Les étapes lyonnaises dans le parcours éditorial du «Clamadès»

    Directory of Open Access Journals (Sweden)

    Fanny Maillet

    2014-12-01

    Full Text Available Le roman de Cleomadès d’Adenet le Roi a donné lieu au XVe siècle à deux mises en prose, dont seule la plus courte est passée sous les presses, tant françaises qu’espagnoles. L’examen des imprimés lyonnais veut montrer que, parallèlement aux productions troyennes et parisiennes, un Clamadès spécifiquement lyonnais semble se dessiner, depuis l’editio princeps de Guillaume Le Roy, pour atteindre son aboutissement au début du XVIe siècle. En revanche, le dernier imprimé lyonnais rompt le rapport de succession linéaire et laisse entendre que nous ne disposons aujourd’hui que de quelques témoins d’une tradition bien plus complexe.The romance of Cleomadès by Adenet le Roi was translated into prose twice in the 15th century, but only the shorter, known as Clamadès, was printed in France as well as in Spain. The review of the editions of Clamadès printed in Lyon tends to show that, beside the other French productions, the editio princeps by Guillaume Le Roy has given rise to an independent tradition that culminates in the early 16th century. By way of contrast, the last edition of Clamadès printed in Lyon breaks the straight-line relationship between the different witnesses and implies that we only have a few tracks and trails left from a much more complex tradition.

  19. THE BLACK HOLE CENTRAL ENGINE FOR ULTRA-LONG GAMMA-RAY BURST 111209A AND ITS ASSOCIATED SUPERNOVA 2011KL

    Energy Technology Data Exchange (ETDEWEB)

    Gao, He; You, Zhi-Qiang [Department of Astronomy, Beijing Normal University, Beijing 100875 (China); Lei, Wei-Hua; Xie, Wei, E-mail: gaohe@bnu.edu.cn, E-mail: leiwh@hust.edu.cn [School of Physics, Huazhong University of Science and Technology, Wuhan, 430074 (China)

    2016-08-01

    Recently, the first association between an ultra-long gamma-ray burst (GRB) and a supernova was reported, i.e., GRB 111209A/SN 2011kl, enabling us to investigate the physics of central engines or even progenitors for ultra-long GRBs. In this paper, we inspect the broadband data of GRB 111209A/SN 2011kl. The late-time X-ray light curve exhibits a GRB 121027A-like fallback bump, suggesting a black hole (BH) central engine. We thus propose a collapsar model with fallback accretion for GRB 111209A/SN 2011kl. The required model parameters, such as the total mass and radius of the progenitor star, suggest that the progenitor of GRB 111209A is more likely a Wolf–Rayet star instead of a blue supergiant, and the central engine of this ultra-long burst is a BH. The implications of our results are discussed.

  20. Structure of recombinant Ves v 2 at 2.0 Angstrom resolution

    DEFF Research Database (Denmark)

    Skov, Lars K; Seppälä, Ulla; Coen, Jeremy J F

    2006-01-01

    Wasp venom from Vespula vulgaris contains three major allergens: Ves v 1, Ves v 2 and Ves v 5. Here, the cloning, expression, biochemical characterization and crystal structure determination of the hyaluronidase Ves v 2 from family 56 of the glycoside hydrolases are reported. The allergen...... was expressed in Escherichia coli as an insoluble protein and refolded and purified to obtain full enzymatic activity. Three N-glycosylation sites at Asn79, Asn99 and Asn127 were identified in Ves v 2 from a natural source by enzymatic digestions combined with MALDI-TOF mass spectrometry. The crystal structure...... of recombinant Ves v 2 was determined at 2.0 A resolution and reveals a central (beta/alpha)(7) core that is further stabilized by two disulfide bonds (Cys19-Cys308 and Cys185-Cys197). Based on sequence alignments and structural comparison with the honeybee allergen Api m 2, it is proposed that a conserved...

  1. Demand of authorization to create the 'Flamanville 3' Basis Nuclear Installation. Synthesis report of the technical examination; Demande d'autorisation de creation de l'INB 'Flamanville 3'. Rapport de synthese de l'examen technique

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-02-15

    The present report has for objective to present to the College of Asn a synthesis of the technical instruction piloted by the services of Asn and realized by their technical supports (I.R.S.N., G.P.R., S.P.N.) during the period 2001-2006. After a reminder of the conditions of the examination of the safety options of the project EPR made between 1993 and 2000, this report explains the progress and the modalities of the instruction realized from 2001 till 2006. It draws up then, besides a notice of the Asn services at request of creation authorization, a synthesis of the technical examination realized according to axes presented on the fig 1. Finally, it presents the main lines of the examination that it would stay to realize if the creation authorization was delivered. (N.C.)

  2. Requirement for asparagine in the aquaporin NPA sequence signature motifs for cation exclusion

    DEFF Research Database (Denmark)

    Wree, Dorothea; Wu, Binghua; Zeuthen, Thomas

    2011-01-01

    Two highly conserved NPA motifs are a hallmark of the aquaporin (AQP) family. The NPA triplets form N-terminal helix capping structures with the Asn side chains located in the centre of the water or solute-conducting channel, and are considered to play an important role in AQP selectivity. Although...... interchangeable at both NPA sites without affecting protein expression or water, glycerol and methylamine permeability. However, other mutations in the NPA region led to reduced permeability (S186C and S186D), to nonfunctional channels (N64D), or even to lack of protein expression (S186A and S186T). Using...... electrophysiology, we found that an analogous mammalian AQP1 N76S mutant excluded protons and potassium ions, but leaked sodium ions, providing an argument for the overwhelming prevalence of Asn over other amino acids. We conclude that, at the first position in the NPA motifs, only Asn provides efficient helix cap...

  3. High committee for transparency and information on nuclear safety: meeting of April 29, 2010

    International Nuclear Information System (INIS)

    2010-01-01

    The committee members first discuss the report project concerning the State minister's and OPECST (parliamentary office of scientific and technological choices) chairman's submission regarding the transparency of the nuclear fuel cycle. They discuss the project of a portal web site gathering information on French nuclear power plant operations. They comment the 2009 ASN assessment on medical nuclear activities, the implementation of the Cancer Plan (presentation by the minister of public health, statements by the French nuclear safety authority or ASN, the French institute for radiation protection and nuclear safety or IRSN, and by associations of patients). They discuss the presentation by the ASN of a return on experience after the ATPu event and the presentation by the IRSN of technical form on the criticality risk. Other topics are addressed: extensions of storage sites under construction in La Hague, modification of the Grenelle II bill to improve public consultation in some procedures regarding base nuclear installations

  4. Exploring symbiotic nitrogen fixation and assimilation in pea root nodules by in vivo 15N nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectrometry

    DEFF Research Database (Denmark)

    Scharff, A.M.; Egsgaard, H.; Hansen, P.E.

    2003-01-01

    Nitrogen (N) fixation and assimilation in pea (Pisum sativum) root nodules were studied by in vivo N-15 nuclear magnetic resonance (NMR) by exposing detached nodules to N-15, via a perfusion medium, while recording a time course of spectra. In vivo P-31 NMR spectroscopy was used to monitor...... the physiological state of the metabolically active nodules. The nodules were extracted after the NMR studies and analyzed for total soluble amino acid pools and N-15 labeling of individual amino acids by liquid chromatography-mass spectrometry. A substantial pool of free ammonium was observed by N-15 NMR...... labeling of Asn was observed by liquid chromatography-mass spectrometry, which is consistent with the generally accepted role of Asn as the end product of primary N assimilation in pea nodules. However, the Asn N-15 amino signal was absent in in vivo N-15 NMR spectra, which could be because...

  5. Demand of authorization to create the 'Flamanville 3' Basis Nuclear Installation. Synthesis report of the technical examination

    International Nuclear Information System (INIS)

    2007-02-01

    The present report has for objective to present to the College of Asn a synthesis of the technical instruction piloted by the services of Asn and realized by their technical supports (I.R.S.N., G.P.R., S.P.N.) during the period 2001-2006. After a reminder of the conditions of the examination of the safety options of the project EPR made between 1993 and 2000, this report explains the progress and the modalities of the instruction realized from 2001 till 2006. It draws up then, besides a notice of the Asn services at request of creation authorization, a synthesis of the technical examination realized according to axes presented on the fig 1. Finally, it presents the main lines of the examination that it would stay to realize if the creation authorization was delivered. (N.C.)

  6. The 26. CLI national conference. Conference proceedings

    International Nuclear Information System (INIS)

    Chevet, Pierre-Franck; Niel, Jean-Christophe; Legrand, Henri; Dumont, Jean-Jacques; Lachaume, Jean-Luc; Delalonde, Jean-Claude; Sene, Monique; Le Deaut, Jean Yves; Charles, Thierry; Sasseigne, Philippe; Fournier, Nicolas; Murith, Christophe; Rivasi, Michele; Perissat, Frederic; KESSLER, Emmanuel

    2014-12-01

    This document gathers contributions presented during a conference held in December 2014. After introduction speeches and a focus of some updates by ANCCLI and ASN representatives, this conference comprised two round tables. The first one addressed the continuation of nuclear reactor operation after their fourth safety re-examination, with contributions by representatives of the ASN, of the ANCCLI, of the IRSN, and of EDF. The second one addressed the issue of a European harmonisation regarding actions of protection of populations in case of a nuclear accident, with interventions of representatives of a CLI, of the ASN, of the Swiss federal office for public health, of an NGO (Nuclear Transparency Watch), and of a departmental prefect

  7. Fructose-asparagine is a primary nutrient during growth of Salmonella in the inflamed intestine.

    Directory of Open Access Journals (Sweden)

    Mohamed M Ali

    2014-06-01

    Full Text Available Salmonella enterica serovar Typhimurium (Salmonella is one of the most significant food-borne pathogens affecting both humans and agriculture. We have determined that Salmonella encodes an uptake and utilization pathway specific for a novel nutrient, fructose-asparagine (F-Asn, which is essential for Salmonella fitness in the inflamed intestine (modeled using germ-free, streptomycin-treated, ex-germ-free with human microbiota, and IL10-/- mice. The locus encoding F-Asn utilization, fra, provides an advantage only if Salmonella can initiate inflammation and use tetrathionate as a terminal electron acceptor for anaerobic respiration (the fra phenotype is lost in Salmonella SPI1- SPI2- or ttrA mutants, respectively. The severe fitness defect of a Salmonella fra mutant suggests that F-Asn is the primary nutrient utilized by Salmonella in the inflamed intestine and that this system provides a valuable target for novel therapies.

  8. One-step simultaneous differential scanning calorimetry-FTIR microspectroscopy to quickly detect continuous pathways in the solid-state glucose/asparagine Maillard reaction.

    Science.gov (United States)

    Hwang, Deng-Fwu; Hsieh, Tzu-Feng; Lin, Shan-Yang

    2013-01-01

    The stepwise reaction pathway of the solid-state Maillard reaction between glucose (Glc) and asparagine (Asn) was investigated using simultaneous differential scanning calorimetry (DSC)-FTIR microspectroscopy. The color change and FTIR spectra of Glc-Asn physical mixtures (molar ratio = 1:1) preheated to different temperatures followed by cooling were also examined. The successive reaction products such as Schiff base intermediate, Amadori product, and decarboxylated Amadori product in the solid-state Glc-Asn Maillard reaction were first simultaneously evidenced by this unique DSC-FTIR microspectroscopy. The color changed from white to yellow-brown to dark brown, and appearance of new IR peaks confirmed the formation of Maillard reaction products. The present study clearly indicates that this unique DSC-FTIR technique not only accelerates but also detects precursors and products of the Maillard reaction in real time.

  9. Progress report: nuclear safety and radiation protection in France in 2005

    International Nuclear Information System (INIS)

    2007-01-01

    The Asn (Nuclear safety authority) considers that 2005 was a satisfactory year in terms of nuclear safety and radiation protection. However, further progress can and must be made. 2005 was a year of great progress for the Asn as it consolidated its organisation and working methods, in accordance with the 2005-2007 strategic plan it set for itself. The Asn continued progress in the field of radiation protection has given rise to various new regulations to improve the legislative and regulatory framework in this area. 2005 was marked by significant progress in the process of harmonizing national nuclear safety policies Against a backdrop of the preparation of a bill on management of radioactive materials and waste, to be presented to Parliament in March 2006, 2005 was a year of important milestones. The Asn control activities encompass the following seven areas: development of general regulations for nuclear safety and radiation protection; management of individual authorization requests and receipt of declarations; inspection of nuclear activities; organisation of radiological surveillance of individuals and of the environment; preparation for management of emergency situations and implementation if necessary; contribution to public information on nuclear safety and radiation protection; determination of the French position within international community. Main topics in 2005: government bill on transparency and security in the nuclear field; the challenges and ambitions of the Asn; controlling exposure to radon; EPR Reactor Project Safety; working towards a law on radioactive waste in 2006; I.R.R.T.: an international audit of Asn in 2006; harmonization of nuclear safety in Europe; Chernobyl: what has been achieved over the past 20 years; informing the Public; internal authorizations. (N.C.)

  10. The transport safety of radioactive matters

    International Nuclear Information System (INIS)

    Landier, D.; Louet, Ch.A.; Robert, Ch.; Binet, J.; Malesys, P.; Pourade, C.; Le Meur, A.; Robert, M.; Turquet de Beauregard, G.Y.; Hello, E.; Laumond, A.; Regnault, Ph.; Gourlay, M.; Bruhl, G.; Malvache, P.; Dumesnil, J.; Cohen, B.; Sert, G.; Pain, M.; Green, L.; Hartenstein, M.; Stewart, J.; Cottens, E.; Liebens, M.; Marignac, Y.

    2007-01-01

    Since the control of transport of radioactive materials was given to A.S.N. 10 years ago, A.S.N. has strengthened the radioactive material transport inspections, in particular of the designers, manufacturers, carriers and consignors. A.S.N. has implemented INES scale for incidents during transport. It has participated as much as possible to IAEA working groups in order to improve the international regulatory framework. And, supported by I.R.S.N., A.S.N. has performed a periodic safety review of existing package models and has approved new models incorporating innovative design features. Finally, A.S.N. has tested its emergency responses to procedures to an accident involving the transport of radioactive materials. All these actions taken together have led to improvement in and reinforcement of the safety culture among the transport operators; this has been acknowledged by a recent audit T.R.A.N.S.A.S. performed by IAEA. In spite of all these actions, there are not approved by the competent authority. As A.S.N. is in charge of every field in radioprotection, this should help to intensify the control. In addition, the different kinds of transport are also tackled as rail transport with S.N.C.F. radiological risk training, air transport through nuclear medicine. Some experience feedback are given such radioactive waste transport to the storage facilities in the Aube or how to protect the population after a nuclear transport incident with the O.R.S.E.C.-T.M.S. plans. (N.C.)

  11. The transport safety of radioactive matters; La surete des transports des matieres radioactives

    Energy Technology Data Exchange (ETDEWEB)

    Landier, D.; Louet, Ch.A.; Robert, Ch. [Autorite de Surete Nucleaire, 75 - Paris (France); Binet, J. [Commission europeenne, DG Energie et transports, Bruxelles (Belgium); Malesys, P. [TN International, 75 - Paris (France); Pourade, C. [Societe Dangexpress, 78 - St Remy l' Honore (France); Le Meur, A.; Robert, M. [Societe Nationale des Chemins de fer Francais, 75 - Paris (France); Turquet de Beauregard, G.Y.; Hello, E. [CIS bio, 91 - Gif sur Yvette (France); Laumond, A. [Electricite de France (EDF), 75 - Paris (France); Regnault, Ph.; Gourlay, M. [AREVA NC, 78 - Velizy Villacoublay (France); Bruhl, G. [CEA Fontenay-aux-Roses, Dir. de la Protection et de la Surete Nucleaire, 92 (France); Malvache, P.; Dumesnil, J. [CEA Saclay, Dir. de l' Energie Nucleaire (DEN), 91 - Gif sur Yvette (France); Cohen, B. [Agence Nationale pour la Gestion des Dechets Radioactifs (ANDRA), 92 - Chatenay Malabry (France); Sert, G. [Institut de Radioprotection et de Surete Nucleaire (IRSN), 92 - Clamart (France); Pain, M. [Ministere de l' Interieur, et de l' Amenagement du Territoire, Dir. de la Defense et la Securite Civiles, 75 - Paris (France); Green, L.; Hartenstein, M. [World Nuclear Transport Institute, London (United Kingdom); Stewart, J. [Ministere des Transport, Royaume Uni (United Kingdom); Cottens, E.; Liebens, M. [Agence Federale de Controle Nucleaire (Belgium); Marignac, Y. [Wise, 75 - Paris (France)

    2007-02-15

    Since the control of transport of radioactive materials was given to A.S.N. 10 years ago, A.S.N. has strengthened the radioactive material transport inspections, in particular of the designers, manufacturers, carriers and consignors. A.S.N. has implemented INES scale for incidents during transport. It has participated as much as possible to IAEA working groups in order to improve the international regulatory framework. And, supported by I.R.S.N., A.S.N. has performed a periodic safety review of existing package models and has approved new models incorporating innovative design features. Finally, A.S.N. has tested its emergency responses to procedures to an accident involving the transport of radioactive materials. All these actions taken together have led to improvement in and reinforcement of the safety culture among the transport operators; this has been acknowledged by a recent audit T.R.A.N.S.A.S. performed by IAEA. In spite of all these actions, there are not approved by the competent authority. As A.S.N. is in charge of every field in radioprotection, this should help to intensify the control. In addition, the different kinds of transport are also tackled as rail transport with S.N.C.F. radiological risk training, air transport through nuclear medicine. Some experience feedback are given such radioactive waste transport to the storage facilities in the Aube or how to protect the population after a nuclear transport incident with the O.R.S.E.C.-T.M.S. plans. (N.C.)

  12. A novel small molecule inhibitor of influenza A viruses that targets polymerase function and indirectly induces interferon.

    Directory of Open Access Journals (Sweden)

    Mila Brum Ortigoza

    Full Text Available Influenza viruses continue to pose a major public health threat worldwide and options for antiviral therapy are limited by the emergence of drug-resistant virus strains. The antiviral cytokine, interferon (IFN is an essential mediator of the innate immune response and influenza viruses, like many viruses, have evolved strategies to evade this response, resulting in increased replication and enhanced pathogenicity. A cell-based assay that monitors IFN production was developed and applied in a high-throughput compound screen to identify molecules that restore the IFN response to influenza virus infected cells. We report the identification of compound ASN2, which induces IFN only in the presence of influenza virus infection. ASN2 preferentially inhibits the growth of influenza A viruses, including the 1918 H1N1, 1968 H3N2 and 2009 H1N1 pandemic strains and avian H5N1 virus. In vivo, ASN2 partially protects mice challenged with a lethal dose of influenza A virus. Surprisingly, we found that the antiviral activity of ASN2 is not dependent on IFN production and signaling. Rather, its IFN-inducing property appears to be an indirect effect resulting from ASN2-mediated inhibition of viral polymerase function, and subsequent loss of the expression of the viral IFN antagonist, NS1. Moreover, we identified a single amino acid mutation at position 499 of the influenza virus PB1 protein that confers resistance to ASN2, suggesting that PB1 is the direct target. This two-pronged antiviral mechanism, consisting of direct inhibition of virus replication and simultaneous activation of the host innate immune response, is a unique property not previously described for any single antiviral molecule.

  13. Progress report: nuclear safety and radiation protection in France in 2005; Rapport annuel: la surete nucleaire et la radioprotection en France en 2005

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-07-01

    The Asn (Nuclear safety authority) considers that 2005 was a satisfactory year in terms of nuclear safety and radiation protection. However, further progress can and must be made. 2005 was a year of great progress for the Asn as it consolidated its organisation and working methods, in accordance with the 2005-2007 strategic plan it set for itself. The Asn continued progress in the field of radiation protection has given rise to various new regulations to improve the legislative and regulatory framework in this area. 2005 was marked by significant progress in the process of harmonizing national nuclear safety policies Against a backdrop of the preparation of a bill on management of radioactive materials and waste, to be presented to Parliament in March 2006, 2005 was a year of important milestones. The Asn control activities encompass the following seven areas: development of general regulations for nuclear safety and radiation protection; management of individual authorization requests and receipt of declarations; inspection of nuclear activities; organisation of radiological surveillance of individuals and of the environment; preparation for management of emergency situations and implementation if necessary; contribution to public information on nuclear safety and radiation protection; determination of the French position within international community. Main topics in 2005: government bill on transparency and security in the nuclear field; the challenges and ambitions of the Asn; controlling exposure to radon; EPR Reactor Project Safety; working towards a law on radioactive waste in 2006; I.R.R.T.: an international audit of Asn in 2006; harmonization of nuclear safety in Europe; Chernobyl: what has been achieved over the past 20 years; informing the Public; internal authorizations. (N.C.)

  14. Organizing a complex transport while guaranteeing safety and transparency: the example of vitrified wastes sent back to Germany

    International Nuclear Information System (INIS)

    Krochmaluk, Julie; Lebrun, Marc; Delmestre, Alain; Barbey, Pierre; Bonvalot, Vanessa; Belleville, Didier; Rollinger, Patrice; Alter, Ulrich

    2012-01-01

    This chapter is made of several small articles entitled: - 'Les controles avant le depart du convoi franco-allemand' (Controls before the departure of the French-German convoy); - 'La supervision des transports de matieres radioactives: une approche extensive de la surete des operations' (The supervision of radioactive material transports: an extended approach of operation safety); - 'Le dispositif d'information du public sur le transport des substances radioactives mis en place par l'ASN' (The plan implemented by the ASN for public information on nuclear materials transport); - 'Le recours de l'ASN a l'expertise de l'ACRO sur le convoi de colis de dechets vitrifies vers l'Allemagne' (The appeal of the ASN to the ACRO's expertise on the vitrified parcel convoy to Germany); - 'La participation du collectif STOP-EPR a l'inspection de l'ASN du convoi de colis de dechets vitrifies vers l'Allemagne' (The participation of the STOP-EPR collective to the control by the ASN of the vitrified parcel convoy to Germany); - 'La securite et la surete: des valeurs que le Groupe SNCF partage' (Security and safety: values which are shared by the SNCF Group); - 'Le transport de matieres nucleaires entre la France et l'Allemagne au cours des 40 dernieres annees' (Transport of nuclear materials between France and Germany during the last 40 years). Addressing the case of vitrified wastes transported back to Germany, the articles indicate the controls performed in France before convoy departure, discuss the approach adopted for the safety of radioactive material transport, comment the management of public information by the ASN for such transports, comment the intervention of the ACRO (Association for the control of radioactivity in western France) on the request of the ASN and the participation of a collective group (STOP-EPR) to the inspection of a convoy, outline the commitment of the French Railways (SNCF) in terms of security and safety, and give a qualitative and quantitative

  15. Decision no. 2011-DC-0216 of the French nuclear safety authority from May 5, 2011, ordering the Laue Langevin Institute to proceed to a complementary safety evaluation of its basic nuclear facility (high flux reactor - INB no. 67) in the eyes of the Fukushima Daiichi nuclear power plant accident

    International Nuclear Information System (INIS)

    2011-01-01

    As a consequence of the accident of the Fukushima Daiichi nuclear power plant (Japan), the French Prime Minister entrusted the French nuclear safety authority (ASN) with the mission to carry out a safety analysis re-evaluation of the French nuclear facilities, and in particular the nuclear power plants. A decision has been addressed by the ASN to each nuclear operator with the specifications of this safety re-evaluation analysis and the list of facilities in concern. This document is the decision addressed to the Laue Langevin Institute, operator of the high flux research reactor (RHF) of Grenoble (France). (J.S.)

  16. Decision no. 2011-DC-0215 of the French nuclear safety authority from May 5, 2011, ordering ITER Organization to proceed to a complementary safety evaluation of its basic nuclear facility in the eyes of the Fukushima Daiichi nuclear power plant accident

    International Nuclear Information System (INIS)

    2011-01-01

    As a consequence of the accident of the Fukushima Daiichi nuclear power plant (Japan), the French Prime Minister entrusted the French nuclear safety authority (ASN) with the mission to carry out a safety analysis re-evaluation of the French nuclear facilities, and in particular the nuclear power plants. A decision has been addressed by the ASN to each nuclear operator with the specifications of this safety re-evaluation analysis and the list of facilities in concern. This document is the decision addressed to the ITER Organization, operator of the ITER tokamak facility of Cadarache (France). (J.S.)

  17. Decision no. 2011-DC-0222 of the French nuclear safety authority from May 5, 2011, ordering the Comurhex company to proceed to a complementary safety evaluation of some of its basic nuclear facilities in the eyes of the Fukushima Daiichi nuclear power plant accident

    International Nuclear Information System (INIS)

    2011-01-01

    As a consequence of the accident of the Fukushima Daiichi nuclear power plant (Japan), the French Prime Minister entrusted the French nuclear safety authority (ASN) with the mission to carry out a safety analysis re-evaluation of the French nuclear facilities, and in particular the nuclear power plants. A decision has been addressed by the ASN to each nuclear operator with the specifications of this safety re-evaluation analysis and the list of facilities in concern. This document is the decision addressed to Comurhex company, operator of the Tricastin uranium conversion plant (France). (J.S.)

  18. Decision no. 2011-DC-0223 of the French nuclear safety authority from May 5, 2011, ordering the MELOX SA company to proceed to a complementary safety evaluation of some of its basic nuclear facilities in the eyes of the Fukushima Daiichi nuclear power plant accident

    International Nuclear Information System (INIS)

    2011-01-01

    As a consequence of the accident of the Fukushima Daiichi nuclear power plant (Japan), the French Prime Minister entrusted the French nuclear safety authority (ASN) with the mission to carry out a safety analysis re-evaluation of the French nuclear facilities, and in particular the nuclear power plants. A decision has been addressed by the ASN to each nuclear operator with the specifications of this safety re-evaluation analysis and the list of facilities in concern. This document is the decision addressed to MELOX SA company, operator of the Melox MOX fuel fabrication plant of Marcoule (France). (J.S.)

  19. Decision no. 2011-DC-0218 of the French nuclear safety authority from May 5, 2011, ordering the EURODIF SA company to proceed to a complementary safety evaluation of some of its basic nuclear facilities in the eyes of the Fukushima Daiichi nuclear power plant accident

    International Nuclear Information System (INIS)

    2011-01-01

    As a consequence of the accident of the Fukushima Daiichi nuclear power plant (Japan), the French Prime Minister entrusted the French nuclear safety authority (ASN) with the mission to carry out a safety analysis re-evaluation of the French nuclear facilities, and in particular the nuclear power plants. A decision has been addressed by the ASN to each nuclear operator with the specifications of this safety re-evaluation analysis and the list of facilities in concern. This document is the decision addressed to the EURODIF SA company, operator of the George Besse I uranium enrichment plant of the Tricastin site (France). (J.S.)

  20. Decision no. 2011-DC-0224 of the French nuclear safety authority from May 5, 2011, ordering the French atomic energy and alternative energies commission (CEA) to proceed to a complementary safety evaluation of some of its basic nuclear facilities in the eyes of the Fukushima Daiichi nuclear power plant accident

    International Nuclear Information System (INIS)

    2011-01-01

    As a consequence of the accident of the Fukushima Daiichi nuclear power plant (Japan), the French Prime Minister entrusted the French nuclear safety authority (ASN) with the mission to carry out a safety analysis re-evaluation of the French nuclear facilities, and in particular the nuclear power plants. A decision has been addressed by the ASN to each nuclear operator with the specifications of this safety re-evaluation analysis and the list of facilities in concern. This document is the decision addressed to the French atomic energy commission (CEA). (J.S.)