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Sample records for guaiano sesquiterpene estudo

  1. Agarofuran sesquiterpenes from Schaefferia argentinensis.

    Science.gov (United States)

    García, Manuela E; Motrich, Rubén D; Caputto, Beatriz L; Sánchez, Marianela; Palermo, Jorge A; Estévez-Braun, Ana; Ravelo, Angel G; Nicotra, Viviana E

    2013-10-01

    Sixteen dihydro-β-agarofuran sesquiterpenes were isolated from the aerial parts of Schaefferia argentinensis Speg. Their structures were determined by a combination of 1D and 2D NMR and MS techniques. The in vitro antiproliferative activity of the major sesquiterpenes was examined in T47D, MCF7, and MDA-MB231 human cancer cell lines, but was found to be marginal. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Sesquiterpenes from Oplopanax horridus.

    Science.gov (United States)

    Inui, Taichi; Wang, Yuehong; Nikolic, Dejan; Smith, David C; Franzblau, Scott G; Pauli, Guido F

    2010-04-23

    From the anti-TB active fractions of the inner stem bark of Oplopanax horridus, two new heterocyclic nerolidol derivatives, 3,10-epoxy-3,7,11-trimethyldodeca-1,6-dien-11-ol, named neroplomacrol (1), and rel-(3S,6R,7S,10R)-7,10-epoxy-3,7,11-trimethyldodec-1-ene-3,6,11-triol, named neroplofurol (2), were isolated together with oplopandiol (3), falcarindiol (4), and sesamin (5). Extensive spectroscopic analysis revealed that 1 possesses a novel 3,10-oxanonacyclic ring system. Computer-iterated full spin system analysis led to the generation of (1)H NMR fingerprints that will facilitate future dereplication of analogues by providing characteristic spin-spin coupling patterns. The full spin analysis of 5 revealed asymmetric coupling patterns among the chemically equivalent spins, thus confirming the magnetic asymmetry of 5. It was further demonstrated that (1)H NMR fingerprints and MS data enable dereplication of isolates at a submilligram levels including their relative configuration. Countercurrent concentration of the anti-TB activity of the ethnobotanical O. horridus versus the Mycobacterium tuberculosis Erdman strain led to polyynes 3 and 4 as main anti-TB active principles. Their synergistic behavior is linked to a complex fraction containing the new nerolidiol sesquiterpenes, 1 and 2, as phytochemical marker compounds.

  3. New sesquiterpenes from Euonymus europaeus (Celastraceae).

    Science.gov (United States)

    Descoins, Charles; Bazzocchi, Isabel López; Ravelo, Angel Gutiérrez

    2002-02-01

    A new sesquiterpene evoninate alkaloid (1), and two sesquiterpenes (2, 3) with a dihydro-beta-agarofuran skeleton, along with three known sesquiterpenes (4-6), were isolated from the seeds of Euonymus europaeus. Their structures were elucidated by high resolution mass analysis, and one- and two-dimensional (1D and 2D) NMR spectroscopy, including homonuclear and heteronuclear correlation [correlation spectroscopy (COSY), rotating frame Overhauser enhancement spectroscopy (ROESY), heteronuclear single quantum coherence (HSQC), and heteronuclear multiple bond correlation (HMBC)] experiments.

  4. Sesquiterpenes from the Formosan stolonifer Tubipora musica.

    Science.gov (United States)

    Duh, C Y; Chen, K J; El-Gamal, A A; Dai, C F

    2001-11-01

    Eight new sesquiterpenes, tubipolides A-G (1-7) and tubiporone (8) (novel carbon skeleton), and a known sesquiterpene, spirotubipolide, have been isolated from the Formosan stolonifer Tubipora musica. The structures of compounds 1-8 were determined by 1D and 2D NMR spectral analysis.

  5. Sesquiterpenes of Amazonian Piper species*

    OpenAIRE

    SANTOS, Alberdan S; ANDRADE, Eloisa H. A; ZOGHBI, Maria das Graças B; LUZ, Arnaldo I. R; MAIA, José G. S

    1998-01-01

    The essential oils from leaves and thin branches of Piper amapense, Piper ducket and Piper bartlingianum were analysed by GC/MS and all volatile compounds were identified as sesquiterpenes. The main constitutents identified in the oil of P. amapense were trans-caryophyllene (25.0%), caryophyllene oxide (17.0%) and β-selinene (15.0%). The oil of P. duckei was dominated by trans-caryophyllene (23.5%), caryophyllene oxide (18.4%), β-eudesmol (9.4%) and a-eudesmol (9.1%). The major components fou...

  6. Immunosuppressive sesquiterpenes from Buddleja daviddi.

    Science.gov (United States)

    Zhang, Wen; Yao, Zhi; Zhang, Yan Wen; Zhang, Xing Xiang; Takaishi, Yoshihisa; Duan, Hong Quan

    2010-11-01

    Six new sesquiterpenes, 2,6(12),10-humulatrien-7β-ol-1-one (1), 2 α-acetoxy-5α-methoxy-enantio-caryophylla-8(15)-en-3-one (2), 2α-acetoxy-5α-hydroxy-enantio-caryophylla-8(15)-en-3-one (3), 2α-acetoxy-4β,5α-hydroxy-enantio-caryophylla-8(15)-en-3-one ( 4), 2α-acetoxy-4β,5β-hydroxy-enantio-caryophylla-8(15)-en-3-one (5), 2β-acetoxy-4-caryophyllen-8β-ol-3-one (6), and nineteen known compounds were isolated from the ethanol extract of Buddleja daviddi. The structures were elucidated by spectroscopic methods. Compounds 8-11, 14, 16, 17, and 20 showed significant immunosuppressive activities, and 8-11 and 14 were cytotoxic on HeLa and L929 cell lines. © Georg Thieme Verlag KG Stuttgart · New York.

  7. Sesquiterpene lactones in two endemic Anthemis species

    International Nuclear Information System (INIS)

    Staneva, J.; Todorova, M.; Evstatieva, L.; Dimitrov, D.

    2006-01-01

    The aerial parts of Anthemis stribrnyi subsp.tracica and A. macedonica afforded four and nine sesquiterpene lactones, respectively. Their structures were elucidated by spectral methods. Acetylisospiciformin is a new natural compound. (authors)

  8. Sesquiterpene lactones from Crepis setosa Hal.

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    Wanda Kisiel

    2014-01-01

    Full Text Available The roots of Crepis setosa Hall. afforded four sesquiterpene lactones, among them three glycosides, all derived from 11β,13-dihydrozaluzanin C and not previously reported from this species.

  9. Microscopic fungi as significant sesquiterpene emission sources

    Science.gov (United States)

    HorváTh, Eszter; Hoffer, AndráS.; SebőK, Flóra; Dobolyi, Csaba; Szoboszlay, SáNdor; Kriszt, BaláZs; GelencséR, AndráS.

    2011-08-01

    Among the volatile organic compounds emitted by vegetation, isoprene, monoterpenes, sesquiterpenes, and their derivatives are thought to contribute to secondary organic aerosol formation. Although it is well known that microscopic fungi globally turn over vast amount of carbon by decomposing the organic matter in the soil, vegetation is considered as the exclusive source of biogenic secondary organic aerosol precursors in various atmospheric models. Secondary fungal metabolites including sesquiterpenes have been recognized as characteristic volatile organic compounds emitted by fungi. In the present study, we investigated the rates of sesquiterpene emission of microscopic fungi to establish their potential significance compared to those from vegetation. To sample the headspace of the pure culture of some common fungi, we used an aseptic flow-through apparatus designed for solid phase microextraction in our laboratory. The identified sesquiterpenes in the headspace extracts were quantified for eight strains of microscopic fungi belonging to four different genera. Our results showed that microscopic fungi emit a considerable amount of sesquiterpenes. Based on our first estimations microscopic fungi may be considered as potentially significant sesquiterpene emission sources whose contribution to secondary organic aerosol formation may be comparable to that of vegetation.

  10. Sesquiterpenes from the Endophyte Glomerella cingulata.

    Science.gov (United States)

    Liu, Yunbao; Li, Yong; Liu, Zhen; Li, Li; Qu, Jing; Ma, Shuanggang; Chen, Ridao; Dai, Jungui; Yu, Shishan

    2017-10-27

    From the cultured endophytic fungus Glomerella cingulata isolated from a toxic plant, Gelsemium elegans, one new phenanthrene (1), four new sesquiterpenes (2-5), and three known sesquiterpenes (6-8) were isolated. Their structures were elucidated using spectroscopic methods. Based on the ECD calculations, the absolute configurations of the new compounds were determined. Compounds 2, 4, and 5 inhibited lipopolysaccharide (LPS)-induced NO production in BV2 cells by 50.6, 36.1, and 29.4%, respectively, at 1 μM (positive control curcumin, IC 50 = 4.0 μM).

  11. Chicory cultivars differ in sesquiterpene lactone composition

    Science.gov (United States)

    Chicory (Cichorium intybus L.) is a valuable forage species for small ruminants because it is nutritious and contains sesquiterpene lactones (SLs) that have anthelmintic activity. Three SLs, lactucin (lac), 8-deoxylactucin (dol), and lactucopicrin (lpic), occur in chicory leaves. Comprehensive dat...

  12. Total synthesis of insect antifeedant drimane sesquiterpenes

    NARCIS (Netherlands)

    Jansen, B.J.M.

    1993-01-01

    The investigations described in this thesis deal with the total synthesis of sesquiterpenes of the drimane family, named for their widespread occurrence in the stem bark of South American Drimys species. These compounds contain the bicyclofarnesol nucleus

  13. A new sesquiterpene from Artemisia vulgaris.

    Science.gov (United States)

    Ragasa, Consolacion Y; de Jesus, Jenny P; Apuada, Mary Jane; Rideout, John A

    2008-10-01

    The dichloromethane extract of the air-dried leaves of Artemisia vulgaris afforded a new sesquiterpene 1, caryophyllene oxide, phytyl fatty acid esters, squalene, stigmasterol and sitosterol. The structure of 1 was elucidated by extensive one- and two-dimensional nuclear magnetic resonance spectroscopy.

  14. Cytotoxic sesquiterpene lactones from Inula britannica.

    Science.gov (United States)

    Park, E J; Kim, J

    1998-12-01

    Cytotoxicity-guided fractionation of the flowers of Inula britannica led to the isolation of four sesquiterpene lactones, 4 alpha, 6 alpha-dihydroxyeudesman-8 beta, 12-olide (1), ergolide (2), 8-epi-helenalin (3), and bigelovin (4). Compound 1 was isolated as a new natural product. These compounds showed cytotoxicity against human tumor cell lines.

  15. Temperature dependencies of Henry's law constants for different plant sesquiterpenes.

    Science.gov (United States)

    Copolovici, Lucian; Niinemets, Ülo

    2015-11-01

    Sesquiterpenes are plant-produced hydrocarbons with important ecological functions in plant-to-plant and plant-to-insect communication, but due to their high reactivity they can also play a significant role in atmospheric chemistry. So far, there is little information of gas/liquid phase partition coefficients (Henry's law constants) and their temperature dependencies for sesquiterpenes, but this information is needed for quantitative simulation of the release of sesquiterpenes from plants and modeling atmospheric reactions in different phases. In this study, we estimated Henry's law constants (Hpc) and their temperature responses for 12 key plant sesquiterpenes with varying structure (aliphatic, mono-, bi- and tricyclic sesquiterpenes). At 25 °C, Henry's law constants varied 1.4-fold among different sesquiterpenes, and the values were within the range previously observed for monocyclic monoterpenes. Hpc of sesquiterpenes exhibited a high rate of increase, on average ca. 1.5-fold with a 10 °C increase in temperature (Q10). The values of Q10 varied 1.2-fold among different sesquiterpenes. Overall, these data demonstrate moderately high variation in Hpc values and Hpc temperature responses among different sesquiterpenes. We argue that these variations can importantly alter the emission kinetics of sesquiterpenes from plants. Copyright © 2015 Elsevier Ltd. All rights reserved.

  16. Sesquiterpene lactones in a hairy root culture of Cichorium intybus.

    Science.gov (United States)

    Malarz, Janusz; Stojakowska, Anna; Kisiel, Wanda

    2002-01-01

    A transformed root culture of Cichorium intybus L. (Asteraceae) was found to produce sesquiterpene lactones of guaiane and germacrane type. Lactucopicrin, 8-desoxylactucin and three sesquiterpene lactone glycosides: crepidiaside B, sonchuside A and ixerisoside D were isolated from the roots. The yield of 8-desoxylactucin reached 0.03 g l(-1) at the early stationary phase of the culture.

  17. Analysis of leaf surface sesquiterpenes in potato varieties.

    Science.gov (United States)

    Szafranek, Beata; Chrapkowska, Karolina; Pawińska, Maria; Szafranek, Janusz

    2005-04-20

    A comparative study of potato leaf sesquiterpenes was carried out. GC, GC-MS, and NMR analyses were used to identify and quantify the sesquiterpenes in the leaf surfaces of 10 potato (Solanum tuberosum) varieties. Two sesquiterpene alcohols and 17 sesquiterpene hydrocarbons were identified and quantitatively determined. The distribution of the sesquiterpenes was found to be variety-specific. The sesquiterpene contents of the different potato varieties were subjected to cluster and principal component analyses. The eight potato varieties of the main chemotype cluster were dominated by beta-caryophyllene (9-148 ng/cm2), germacrene D (2-46 ng/cm2), germacrene D-4-ol (0.4-31 ng/cm2), beta-sesquiphellandrene (1-34 ng/cm2), and an unknown sesquiterpene alcohol III (0.2-37 ng/cm2). Chemometric classification distinguished two varieties, Mila and Vistula, from a major cluster. The Vistula variety was distinguished from the others by its high contents of beta-caryophyllene, alpha-humulene, germacrene D, and germacrene D-4-ol and the Mila variety by beta-elemene, trans-alpha-bergamotene, (Z)-beta-farnesene, (E)-beta-farnesene, trans-beta-bergamotene, beta-sesquiphellandrene, and unknown sesquiterpene alcohols I, II, III.

  18. Flavonoids and sesquiterpenes of Croton pedicellatus Kunth

    International Nuclear Information System (INIS)

    Lopes, Elton Luz; Andrade Neto, Manoel; Silveira, Edilberto Rocha; Pessoa, Otilia Deusdenia Loiola; Braz-Filho, Raimundo

    2012-01-01

    The chemical investigation of the ethanolic extract from leaves of Croton pedicellatus yielded the bis-nor-sesquiterpenes blumenol A and blumenol A glucoside, along with the flavonoids: tiliroside, 6 - O-p-coumaroyl-β-galactopyranosyl- kaempferol, 6 - O-p-coumaroyl-β-glucopyranosyl-3 - methoxy- kaempferol, kaempferol, 3-glucopyranosyl-quercetin and alpinumisoflavone, as well as 4-hydroxy-3,5-dimethoxybenzoic acid. The identification of all isolated compounds was performed by spectrometric methods, including HR-ESI-MS, 1D and 2D NMR experiments, and by comparison with previously-described physical and spectral data. (author)

  19. Natural sesquiterpen lactones as acetylcholinesterase inhibitors

    Directory of Open Access Journals (Sweden)

    HOMA HAJIMEHDIPOOR

    2014-06-01

    Full Text Available Background and the purpose of the study: The amount of elder people who suffer from Alzheimer disease is continuously increasing every year. Cholinesterase inhibitors have shown to be effective in alleviating the symptoms of the disease, thus opening a field of research for these treatments. Herbal products, owning a reputation as effective agents in many biological studies are now drawing attention for inhibiting acetylcholinesterase, in other words, Alzheimer disease. In the present study, the ability of three sesquiterpene lactones from Inula oculus-christi and I. aucheriana to inhibit AChE has been evaluated through Ellman assay.Materials and Methods: Gaillardin and pulchellin C were obtained from I. oculus-christi and britannin from I. aucheriana by chromatographic methods. They were dissolved in methanol in concentration of 3 mg/mL and the AChEI activity of the compounds was determined by Ellman method using Acethylthiocholine iodide as the substrate and 5, 5′-dithiobis-2-nitrobenzoic acid as the reagent, in 96-well plates at 405 nm.Results: AChEI activity of the examined compounds was obtained as 67.0, 25.2 and 10.9% in concentration of 300 µg/L for gaillardin, britannin and pulchellin C, respectively.Conclusion: Among the three sesquiterpene lactones, gaillardin with 67% inhibition of AChE could be considered a good candidate for future Alzheimer studies.

  20. Emissions of Sesquiterpenes from Spruce Sawdust During Drying

    International Nuclear Information System (INIS)

    Granstroem, K.M.

    2006-01-01

    Large amounts of sawdust are dried for production of particleboard and Pellets. During processing of wood (i.e. drying, densification), volatile organic compounds are emitted. These contribute in the presence of nitrogen oxides and sunlight to the formation of ground level ozone and other harmful photo-oxidants. In this study sesquiterpene emissions from the drying of fresh Norway Spruce (Picea abies) sawdust in a continuous spouted bed steam dryer at 140-200 deg C have been investigated, and patterns of covariation between sesquiterpene emissions and drying parameters elucidated. Sesquiterpene emissions was about 20% of the monoterpene emissions. Drying in 200 deg C caused markedly larger sesquiterpene emissions than in 140 deg C or 170 deg C. Whereas a change in moisture content had no notable effect on the amount of sesquiterpenes emitted at high wood moisture contents (25-40%), the sesquiterpene emissions increased considerably as drying proceeded at low wood moisture contents (5-15%). While it has long been known that monoterpenes are a dominant VOC emitted during processing of wood, this study shows that sesquiterpenes are of considerable importance

  1. Biosynthesis of Sesquiterpene Lactones in Pyrethrum (Tanacetum cinerariifolium)

    NARCIS (Netherlands)

    Ramirez, A.M.; Saillard, N.; Yang, T.; Franssen, M.C.R.; Bouwmeester, H.J.; Jongsma, M.A.

    2013-01-01

    The daisy-like flowers of pyrethrum (Tanacetum cinerariifolium) are used to extract pyrethrins, a botanical insecticide with a long history of safe and effective use. Pyrethrum flowers also contain other potential defense compounds, particularly sesquiterpene lactones (STLs), which represent

  2. BIOLOGICALLY ACTIVE COMPOUNDS OF ARTEMISIA ANNUA. SESQUITERPENE LACTONES

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    D. A. Konovalov

    2016-01-01

    Full Text Available Artemisia annua is an herblike annual plant which has been used in Chinese folk medicine for more than 2,000 years. In 1970-s sesquiterpene lactones of artemisinin was isolated from the aboveground part of this plant. Today it is the most efficient known natural and synthetic compound for malaria treatment.The purpose of the study was the review of the information from the open sources about the study for sesquiterpene lactones of Artemisia annua referring to its pharmacological activity.Methods. The study was carried out using informational and search engines (PubMed, ScholarGoogle, library databases (eLibrary, Cyberleninca, and the results of our own researches.Results. It was established that apart from the essential oil and phenolic compounds, aboveground part of Artemisia annua, it contains a significant amount of sesquiterpene lactones. Qualitative content and quantitative composition of sesquiterpene lactones varies depending on the ecological and geographic factors, plants growing phase, cultivation technology, drying methods etc. Well-known pharmacological studies of the extracts from Artemisia annua herb with sesquiterpene lactones, as well as individual compounds of this group characterize this type of raw materials as a perspective source for more profound research.Conclusion. Our analysis of the open materials on the sesquiterpene lactones of Artemisia annua, including phytochemical and pharmacological ones, allows characterization of the Artemisia annua herb as a perspective source for new drugs working out.

  3. Sesquiterpene lactones. XXXVI. Sesquiterpene lactones in several subgenera of the genus Centaurea L.

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    Gerard Nowak

    2014-01-01

    Full Text Available The occurrence of both known and new sesquiterpene lactones was determined in six species classified in different subgenera of Centaurea L. Chlorojanerin, cynaropicrin and janerin were isolated from C. phaeopappoides Bordz. and C. thracica (Janka Hayek. C. marschalliana Spreng. was found to contain acroptilin, chlorojanerin, cebellin D and janerin while C. adjarica Alb. had repin, acroptilin, chlorojanerin, centaurepensin, janerin, repidiolide, cebellin D, E, F and L A new, hitherto underscribed cebellin J quaianolide was found in C. bella Trautv. and another germacranolide, 3α, 15-dihydroxycostunolide was found in C. sphaerocephala subsp. lusitanica (Boiss. et Reuter Nyman.

  4. SESQUITERPENE LACTONES OF LEAVES AND FRUITS OF LAURUS NOBILIS L.

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    D. A. Konovalov

    2014-01-01

    Full Text Available Bay laurel (Laurus nobilis L. is common in the Mediterranean region, Europe and America. Widely cultivated in the countries of the Arabian Peninsula and North Africa. As the basic active substances in plants: essential oil components, sesquiterpene lactones, alkaloids, and phenolic compounds (flavonoids, phenolic acids and lignans are described.In the plant more than 30 sesquiterpene lactones are found. Structural types of these compounds include eudesmanolides, germacranolides, guaianolides.Sesquiterpene lactones isolated from the leaves and fruits of Laurus nobilis, possess antibacterial, antifungal, anti-diabetic, anti-inflammatory, hepatoprotective, neuroprotective and cytotoxic activity. They inhibit the absorption of alcohol increases the activity of hepatic glutathione-S-transferase. Most of these types of activity of sesquiterpene lactones from laurel due to the presence within their structure of α-methylene--butyrolactone part.Thus, sesquiterpene lactones-containing leaves and fruits Laurus nobilis could have been an important source of raw materials for the creation of new medicines

  5. Chemotaxonomy of Pseudowintera: sesquiterpene dialdehyde variants are species markers.

    Science.gov (United States)

    Wayman, Kjirsten A; de Lange, Peter J; Larsen, Lesley; Sansom, Catherine E; Perry, Nigel B

    2010-05-01

    Two sesquiterpene dialdehydes, the 1beta-E-coumaroyl-5alpha-hydroxypolygodial plus the known 1beta-E-cinnamoyl-5alpha-hydroxypolygodial, were isolated from the recently described species Pseudowintera insperata. This discovery is a further example of the rare sesquiterpene dialdehyde coumarate/cinnamate combination being found exclusively in the family Winteraceae. Another sesquiterpene dialdehyde, isopaxidal, with the rare rearranged drimane skeleton, was isolated from Pseudowintera axillaris. The sesquiterpene dialdehyde contents of leaves of 25 individual plants of the four Pseudowintera species, all endemic to New Zealand, were measured by HPLC. P. insperata individuals all had high levels (3.0-6.9% of leaf dry wt.) of the coumarate, P. axillaris had high levels (2.2-6.9%) of paxidal, and Pseudowintera colorata from different areas of New Zealand contained varying levels of polygodial (1.4-2.9%) and 9-deoxymuzigadial (0-2.9%). Therefore the sesquiterpene dialdehydes are good species markers. Copyright 2010 Elsevier Ltd. All rights reserved.

  6. Cytotoxic sesquiterpene lactones and other constituents of Centaurea omphalotricha

    Energy Technology Data Exchange (ETDEWEB)

    Kolli, El Hadj; Leon, Francisco; Benayache, Samir, E-mail: jfleon@ipna.csic.es, E-mail: sbenayache@yahoo.com [Laboratoire de Valorisation des Ressources Naturelles et Synthese de Substances Bioactives, Equipe Associee a l' A.N.D.R.S., Universite Mentouri, Constantine (Algeria); Benayache, Fadila [Laboratoire de Phytochimie et Analyses Physico-Chimiques et Biologiques, Universite Mentouri, Constantine (Algeria); Estevez, Sara; Quintana, Jose; Estevez, Francisco [Departamento de Bioquimica, Unidad Asociada al CSIC, Facultad de Ciencias de la Salud, Universidad de Las Palmas de Gran Canaria (Spain); Instituto Canario de Investigacion del Cancer, Las Palmas de Gran Canaria, Gran Canaria (Spain); Brouard, Ignacio; Bermejo, Jaime [Instituto de Productos Naturales y Agrobiologia, CSIC, La Laguna, Tenerife (Spain)

    2012-05-15

    Phytochemical research of the aerial parts of Centaurea omphalotricha led to the isolation of three new sesquiterpene lactones, 4'-acetyl cynaropicrin, 4'-acetyl cebellin F and 15-acetyl dehydromelitensin, together with twelve known compounds, seven sesquiterpene lactones, two isoprenoids and three flavonoids. The structures of the new compounds were elucidated by means of extensive 1D and 2D NMR, and MS, and by comparison with reported data in the literature. The effect of sesquiterpene lactones on the viability of the human tumor cell lines HL-60 and U937 was also investigated and 3-acetyl cynaropicrin, and 4'-acetyl cynaropicrin were found to be the most cytotoxic compounds against human leukemia cells with an IC{sub 50} values of 2.0 =- 0.9 and 5.1 +- 0.4 {mu}mol L{sup -1}, respectively. (author)

  7. Emerging Anti-Mitotic Activities and Other Bioactivities of Sesquiterpene Compounds upon Human Cells

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    Alessandra Bosco

    2017-03-01

    Full Text Available We review the bio-activities of natural product sesquiterpenes and present the first description of their effects upon mitosis. This type of biological effect upon cells is unexpected because sesquiterpenes are believed to inactivate proteins through Michael-type additions that cause non-specific cytotoxicity. Yet, certain types of sesquiterpenes can arrest cells in mitosis as measured by cell biology, biochemical and imaging techniques. We have listed the sesquiterpenes that arrest cells in mitosis and analyzed the biological data that support those observations. In view of the biochemical complexity of mitosis, we propose that a subset of sesquiterpenes have a unique chemical structure that can target a precise protein(s required for mitosis. Since the process of mitotic arrest precedes that of cell death, it is possible that some sesquiterpenes that are currently classified as cytotoxic might also induce a mitotic arrest. Our analysis provides a new perspective of sesquiterpene chemical biology

  8. Profiling chicory sesquiterpene lactones by high resolution mass spectrometry

    NARCIS (Netherlands)

    Graziani, G.; Ferracane, R.; Sambo, P.; Santagata, S.; Nicoletto, C.; Fogliano, V.

    2015-01-01

    Sesquiterpene lactones (SLs) are the main determinants of radicchio bitterness and the control of their concentrations is a key point for the market value of this product. An innovative analytical approach based on two complementary mass spectrometers, Orbitrap-HRMS and MS/MS, was used for

  9. Bisabolane Type Sesquiterpenes from a Marine Didiscus Sponge

    OpenAIRE

    TAŞDEMİR, Deniz

    2014-01-01

    Two bisabolane type sesquiterpene phenols, (+)-curcuphenol (1) and (+)-curcudiol (2), were isolated from a Philippine marine sponge, Didiscus sp., in addition to b -sitosterol (3) and phenethylamine (4). The structures of the metabolites were established on the basis of spectral evidence (1D- and 2D NMR, [a]D, EIMS). (+)-Curcuphenol (1) showed cytotoxicity, which is indicative of a p53 independent mechanism.

  10. Hydrocarbons sesquiterpenics present in species of Clusia genus

    International Nuclear Information System (INIS)

    Gonzalez, Jaime; Martinez, Eduardo; Moreno, Barbara; Delle Monache, Franco

    1993-01-01

    From fruit extracts of C. sandiensis, C. minor, and C. Criuva and from roots of C. Eugenioides we have obtained by column chromatography, some colorless oil fractions, which contain hydrocarbons sesquiterpenics mainly, and oxygenated derivatives, too. The compounds were identified by gas chromatography and gas chromatography-mass spectrometry

  11. Insight into Biochemical Characterization of Plant Sesquiterpene Synthases

    DEFF Research Database (Denmark)

    Manczak, Tom; Simonsen, Henrik Toft

    2016-01-01

    A fast and reproducible protocol was established for enzymatic characterization of plant sesquiterpene synthases that can incorporate radioactivity in their products. The method utilizes the 96-well format in conjunction with cluster tubes and enables processing of >200 samples a day. Along with ...

  12. Temperature dependencies of Henry’s law constants for different plant sesquiterpenes

    Science.gov (United States)

    Copolovici, Lucian; Niinemets, Ülo

    2018-01-01

    Sesquiterpenes are plant-produced hydrocarbons with important ecological functions in plant-to-plant and plant-to-insect communication, but due to their high reactivity they can also play a significant role in atmospheric chemistry. So far, there is little information of gas/liquid phase partition coefficients (Henry’s law constants) and their temperature dependencies for sesquiterpenes, but this information is needed for quantitative simulation of the release of sesquiterpenes from plants and modeling atmospheric reactions in different phases. In this study, we estimated Henry’s law constants (Hpc) and their temperature responses for 12 key plant sesquiterpenes with varying structure (aliphatic, mono-, bi- and tricyclic sesquiterpenes). At 25 °C, Henry’s law constants varied 1.4-fold among different sesquiterpenes, and the values were within the range previously observed for monocyclic monoterpenes. Hpc of sesquiterpenes exhibited a high rate of increase, on average ca. 1.5-fold with a 10 °C increase in temperature (Q10). The values of Q10 varied 1.2-fold among different sesquiterpenes. Overall, these data demonstrate moderately high variation in Hpc values and Hpc temperature responses among different sesquiterpenes. We argue that these variations can importantly alter the emission kinetics of sesquiterpenes from plants. PMID:26291755

  13. Occupational contact dermatitis toward sesquiterpene lactones in a florist.

    Science.gov (United States)

    Bangha, E; Elsner, P

    1996-09-01

    Many plants and flowers can cause dermatitis in florists, growers, and others working in the industry. In the United States, Alstromeria, also called Peruvian Lily, has frequently been reported to cause hand eczema in floral workers. This interesting report details sesquiterpene lactone allergy in the occupational setting. Fortunately, in this case, it seems that resolution of the dermatitis was achieved fairly rapidly by avoiding the occupational situation. Unfortunately, for many of our patients with airborne compositae dermatitis, there is no way to avoid the allergen; therefore, many of these individuals develop severe, chronic recalcitrant dermatitis requiring aggressive therapy. Diagnosis of this condition is not always easy, as it appears that the sesquiterpene lactone mix allergen that we have commercially available misses a number of cases, at least in the United States. Better characterization of the allergens involved and better supply of allergenic extracts for testing is critical to improve our diagnostic ability.

  14. A dihydro-β-agarofuran sesquiterpene from Maytenus boaria.

    Science.gov (United States)

    Paz, Cristian; von Dossow, Daniela; Tiznado, Victor; Suarez, Sebastián; Cukiernik, Fabio D; Baggio, Ricardo

    2017-06-01

    The natural compound (1S,4S,5S,6R,7R,8R,9R,10S)-6-acetoxy-4,9,10-trihydroxy-2,2,5a,9-tetramethyloctahydro-2H-3,9a-methanobenzo[b]oxepin-5-yl furan-3-carboxylate, C 22 H 30 O 9 , (I), is a β-agarofuran sesquiterpene isolated from the seeds of Maytenus boaria as part of a study of the secondary metabolites from Chilean flora. The compound presents a central structure formed by a decalin system esterified with acetate at site 1 and furan-3-carboxylate at site 9. The chirality of the skeleton can be described as 1S,4S,5S,6R,7R,8R,9R,10S, which is consistent with that suggested by NMR studies. Unlike previously reported structures of sesquiterpenes containing a pure dihydro-β-agarofuran skeleton, (I) exhibits a three-dimensional hydrogen-bonded network.

  15. BODIPY-based fluorescent liposomes with sesquiterpene lactone trilobolide

    Czech Academy of Sciences Publication Activity Database

    Škorpilová, Ludmila; Rimpelová, S.; Jurášek, M.; Buděšínský, Miloš; Lokajová, Jana; Effenberg, R.; Slepička, P.; Ruml, T.; Kmoníčková, Eva; Drašar, P. B.; Wimmer, Zdeněk

    2017-01-01

    Roč. 13, JUL 4 (2017), s. 1316-1324 ISSN 1860-5397 R&D Projects: GA MŠk LD15012; GA MŠk(CZ) LO1304 Institutional support: RVO:61389030 ; RVO:61388963 ; RVO:68378041 Keywords : BODIPY conjugates * Cancer targeting * Drug delivery * Liposomes * Natural compounds * Sesquiterpene lactone trilobolide Subject RIV: CC - Organic Chemistry; FR - Pharmacology ; Medidal Chemistry (UEM-P) OBOR OECD: Organic chemistry; Pharmacology and pharmacy (UEM-P) Impact factor: 2.337, year: 2016

  16. Sesquiterpene lactones and other chemical constituents of Mikania hoehnei R.

    Directory of Open Access Journals (Sweden)

    Chaves Juliana S.

    2003-01-01

    Full Text Available Phytochemical study of Mikania hoehnei yielded lupeyl acetate, stigmasterol, b-sitosterol, campesterol, b-sitosteryl glucopyranoside, stigmasteryl glucopyranoside, benzil 2,6-dimethoxybenzoate, luteolin, kaempferol and two sesquiterpene lactones: dehydrocostuslactone and 8b-hydroxyzaluzanin D. IR, ¹H and 13C NMR and MS spectroscopic analyses and comparisons with previously reported data were used for the identification of these compounds.

  17. Sesquiterpenes of Ocotea lancifolia (Lauraceae); Sesquiterpenos de Ocotea lancifolia (Lauraceae)

    Energy Technology Data Exchange (ETDEWEB)

    Camargo, Maria Jose de; Miranda, Mayker Lazaro Dantas; Kagamida, Camila Miyuki; Rodrigues, Edilene Delphino; Garcez, Fernanda Rodrigues; Garcez, Walmir Silva, E-mail: walmir.garcez@ufms.br [Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS (Brazil). Centro de Ciencias Exatas e Tecnologia. Dept. de Quimica

    2013-10-01

    Six new eremophilane sesquiterpenes, (rel)-4{beta},5{beta},7{beta}-eremophil-9-en-12-oic acid (1), (rel)-4{beta},5{beta},7{beta}-eremophil-1(10)-en-12-oic acid (2), (rel)-4{beta},5{beta},7{beta}-eremophil-1(10)-en-2-oxo-12-oic acid (3), (rel)-4{beta},5{beta},7{beta}-eremophil-9-en-12,8{alpha}-olide (4), (rel)-4{beta},5{beta},7{beta}-eremophil-9-en-12,8{beta}-olide (5) and (rel)-4{beta},5{beta},7{beta}-eremophil-9{alpha},10{alpha}-epoxy-12-oic acid (6), were isolated from the leaves of a specimen of Ocotea lancifolia (Lauraceae). Two other known sesquiterpenes, 4{beta},5{beta},7{beta}-eremophil-11-en- 10{alpha}-ol (7) reported for the first time in the genus Ocotea, and the aromadendrene sesquiterpene, spathulenol (8), were also isolated. Their structures were determined on the basis of spectral data (MS, IR, UV and NMR-1D and 2D) and subsequent comparisons with the literature. (author)

  18. Cytotoxic sesquiterpene lactones from the aerial parts of Inula aucheriana.

    Directory of Open Access Journals (Sweden)

    Ahmad Reza Gohari

    2015-06-01

    Full Text Available Inula aucheriana DC is a member of the family Asteraceae which is known to produce cytotoxic secondary metabolites noted as sesquiterpene lactones. In the present study, sesquiterpene lactones inuchinenolide B, 6-deoxychamissonolide (stevin and 14-acetoxy-1β,5α,7αH-4β-hydroxy-guai-9(10,11(13-dien-12,8α-olide were isolated from I. aucheriana. Inuchinenolide B and 14-acetoxy-1β,5α,7αH-4β-hydroxy-guai-9(10,11(13-dien-12,8α-olide were further evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide assay to demonstrate cytotoxic activity with IC50 values of (56.6, 19.0, (39.0, 11.8, and (55.7, 15.3 μg/mL against HepG-2, MCF-7 and A-549 cells, respectively. The cytotoxic activity of the two evaluated sesquiterpene lactones partly explains the cytotoxic activity that was previously observed for the extracts of Inula aucheriana. The isolated compounds could be further investigated in cancer research studies.

  19. Sesquiterpene lactones from Inula britannica and their cytotoxic and apoptotic effects on human cancer cell lines.

    Science.gov (United States)

    Bai, Naisheng; Lai, Ching-Shu; He, Kan; Zhou, Zhu; Zhang, Li; Quan, Zheng; Zhu, Nanqun; Zheng, Qun Yi; Pan, Min-Hsiung; Ho, Chi-Tang

    2006-04-01

    Three new sesquiterpenes (1-3), together with four known sesquiterpene lactones, were isolated from the flowers of Inula britannica var. chinensis. Structures were established on the basis of high-field 1D and 2D NMR methods supported by HRMS. All sesquiterpene lactones were tested for cytotoxicity as well as apoptotic ratio in human COLO 205, HT 29, HL-60, and AGS cancer cells. Compounds 3 and 4, two alpha-methylene gamma-lactone-bearing sesquiterpenes, were modestly active in these assays.

  20. Oxygenated products of sesquiterpenes in secondary organic aerosol

    Science.gov (United States)

    van Eijck, A.; Kampf, C.; Hoffmann, T.

    2012-04-01

    Secondary organic aerosol (SOA) has a huge impact on air quality and climate change. It influences the Earth radiative budget through absorbing, scattering and reflecting radiation as well as the formation of clouds because the particulates can act as cloud condensation nuclei (CCN). Furthermore, it plays an important role for human health. SOA is formed from gaseous precursors which get oxidized by ozone, OH- and NO3-radicals in the atmosphere. Due to their low vapor pressure these degradation products can nucleate to form new particles or they can condense on existing aerosol particles. Despite the major progress in research during the last few years the actual chemical composition as well as the contribution of various volatile organic compounds (VOCs) to the formation of secondary organic aerosol is still partially unknown. Recent studies indicate that sesquiterpenes play an important role in the formation of SOA because of the low volatility of their oxygenated products (Lee et al., 2006). Their emission is estimated to be about 14,8 Tg per year (Henze et al., 2008), however, these emission rates remain highly uncertain due to the lack of quantitative emission rate measurements. In addition, the knowledge about the actual atmospheric degradation mechanism and the main oxidation products of sesquiterpenes is quite limited. β-Caryophyllene, α-humulene, α-farnesene and β-farnesene are the most abundant sequiterpenes in many sesquiterpene emission profiles. But also aromadendren, α-bergamotene and δ-cadinene and germacrene-D can contribute significantly to some emission profiles (Duhl et al., 2008). To determine the major oxygenated products of sesquiterpenes in SOA, reaction chamber experiments with different sesquiterpenes and ozone were performed in a 100 L reaction chamber. To measure the time dependent formation of initial oxidation products, an APCI-IT-MS was directly connected to the reaction chamber. After 2 hours the APCI-IT-MS was replaced by a

  1. Guaianolides from Cichorium intybus and structure revision of Cichorium sesquiterpene lactones.

    Science.gov (United States)

    Kisiel, W; Zielińska, K

    2001-06-01

    The isolation and structure elucidation of a new lactucopicrin derivative from Cichorium intybus is described, together with the revised structures of several sesquiterpene lactones previously isolated from Cichorium species. The known eudesmanolide magnolialide and the known guaianolide ixerisoside D, reported for the first time from the plant material, along with the previously known chicory sesquiterpene lactones have been also isolated and identified.

  2. Comparison of simultaneous patch testing with parthenolide and sesquiterpene lactone mix

    DEFF Research Database (Denmark)

    Orion, E; Paulsen, Evy; Andersen, Klaus Ejner

    1998-01-01

    Several studies have pointed out that the sesquiterpene lactone (SL) mix is a safe, though inadequate, screen for Compositae allergy. To test the usefulness of the sesquiterpene lactone parthenolide as a supplementary Compositae screening test to the mix, both were included in the standard series...

  3. Ferulsinaic acid, a sesquiterpene coumarin with a rare carbon skeleton from Ferula species.

    Science.gov (United States)

    Ahmed, Ahmed A; Hegazy, Mohamed-Elamir F; Zellagui, Amar; Rhouati, Salah; Mohamed, Tarik A; Sayed, Ahmed A; Abdella, Mohamed A; Ohta, Shinji; Hirata, Toshifumi

    2007-03-01

    Fractionation of methylene chloride extracts of the resin of Ferula vesceritensis and F. sinaica afforded three sesquiterpene coumarins and a glucose derivative. One of them was a sesquiterpene with a rare carbon skeleton. The structures of these compounds were determined by extensive NMR studies, including DEPT, COSY, NOE, HMQC, and HMBC.

  4. Ferulsinaic acid, a sesquiterpene coumarin with a rare carbon skeleton from Ferula species

    OpenAIRE

    Ahmed, Ahmed A.; Hegazy, Mohamed-Elamir F.; Zellagui, Amar; Rhouati, Salah; Mohamed, Tarik A.; Sayed, Ahmed A.; Abdella, Mohamed A.; Ohta, Shinji; Hirata, Toshifumi

    2007-01-01

    Fractionation of methylene chloride extracts of the resin of Ferula vesceritensis and F. sinaica afforded three new sesquiterpene coumarins and a new glucose derivative. One of them was a sesquiterpene with a rare carbon skeleton. The structures of these compounds were determined by extensive NMR studies, including DEPT, COSY, NOE, HMQC, and HMBC.

  5. Two novel eremophilane sesquiterpenes from an endophytic Xylariaceous fungus isolated from leaves of Cupressus lusitanica

    International Nuclear Information System (INIS)

    Amaral, Luciana S.; Rodrigues-Filho, Edson

    2010-01-01

    Two new eremophilane sesquiterpenes, cupressolide A and cupressolide B, along with two known sesquiterpenes, has been characterized from the EtOAc extract of a liquid medium where a Xylariaceous fungus, isolated as an endophytic fungus from health tissues of Cupressus lusitanica leaves, was cultivated. The structures of the isolated compounds were determined by analyses of their MS and NMR spectroscopic data. (author)

  6. A New Eudesmane Sesquiterpene from Nigrospora oryzae, an Endophytic Fungus of Aquilaria sinensis

    Directory of Open Access Journals (Sweden)

    Dongli Li

    2014-07-01

    Full Text Available A new eudesmane-type sesquiterpene, 11 -hydroxy capitulatin B (1 , along with a known related sesquiterpene, capitulatin B (2, was isolated from the endophytic fungus Nigrospora oryzae A8 from Aquilaria sinensis, the only plant resource for agarwood production in China. This research demonstrates that the endophytic fungi from A. sinensis might play a role in the formation of agarwood.

  7. Reactive Uptake of Gaseous Sesquiterpenes on Aqueous Surfaces.

    Science.gov (United States)

    Matsuoka, Kohei; Sakamoto, Yosuke; Hama, Tetsuya; Kajii, Yoshizumi; Enami, Shinichi

    2017-02-02

    Sesquiterpenes emitted from biogenic sources play important roles in atmospheric HOx cycles and new particle formation. Current atmospheric models, however, fail to account for their fates, possibly due to missing heterogeneous sinks. Here we apply interface-specific mass spectrometry to detect carbocation products of the reactive uptake of gaseous sesquiterpenes C 15 H 24 (β-caryophyllene (β-C), α-humulene (α-H), and alloaromadendrene (a-d)) on the surface of aqueous microjets as functions of water acidity and gas concentration. We find that these gases are effectively protonated to C 15 H 25 + upon colliding with the surface of pH < 5 water microjets. We determine inflection points from plots of product yields vs bulk pH: pH 1/2 = 4.17 ± 0.05, 4.28 ± 0.06, and 4.36 ± 0.19, and kinetic isotope effects (KIEs) from H 2 O/D 2 O (1:1 = vol/vol) experiments: KIE = 2.31 ± 0.08, 1.95 ± 0.05, and 2.71 ± 0.11, for β-C, α-H, and a-d, respectively. These results are analyzed vis-a-vis previous reports on isoprene and monoterpenes experiments. We estimate 6.2 × 10 -5 ≤ γ ≤ 3.1 × 10 -4 for the reactive uptake of gaseous sesquiterpenes on acidic (1 < pH < 3) water surfaces. The atmospheric implications of present findings are discussed.

  8. Tremulane sesquiterpenes from cultures of the basidiomycete Irpex lacteus.

    Science.gov (United States)

    Ding, Jian-Hai; Li, Zheng-Hui; Feng, Tao; Liu, Ji-Kai

    2018-03-01

    Five new tremulane sesquiterpenes, named irlactins F-J (1-5), were isolated from cultures of the basidiomycete Irpex lacteus together with two known analogues (6 and 7). Structures and relative configurations of compounds 1-5 were elucidated by spectroscopic data analysis. Compund 4 exhibited moderate cytotoxicities on HL-60, SMMC-7721, A-549, MCF-7, and SW480 cells with IC 50 values of 16.23, 20.40, 25.55, 19.05, and 18.58μM, respectively. Copyright © 2018 Elsevier B.V. All rights reserved.

  9. Biosynthesis of sesquiterpene lactones in pyrethrum (Tanacetum cinerariifolium)

    DEFF Research Database (Denmark)

    Ramirez, Aldana M; Saillard, Nils; Yang, Ting

    2013-01-01

    The daisy-like flowers of pyrethrum (Tanacetum cinerariifolium) are used to extract pyrethrins, a botanical insecticide with a long history of safe and effective use. Pyrethrum flowers also contain other potential defense compounds, particularly sesquiterpene lactones (STLs), which represent...... problematic allergenic residues in the extracts that are removed by the pyrethrum industry. The STLs are stored in glandular trichomes present on the pyrethrum achenes, and have been shown to be active against herbivores, micro-organisms and in the below-ground competition with other plants. Despite...

  10. In Vitro Antitumor Activity of Sesquiterpene Lactones from Lychnophora trichocarpha

    Directory of Open Access Journals (Sweden)

    D.A. Saúde-Guimarães

    2014-06-01

    Full Text Available The sesquiterpene lactones lychnopholide and eremantholide C were isolated from Lychnophora trichocarpha Spreng. (Asteraceae, which is a plant species native to the Brazilian Savannah or Cerrado and popularly known as arnica. Sesquiterpene lactones are known to present a variety of biological activities including antitumor activity. The present paper reports on the evaluation of the in vitro antitumor activity of lychnopholide and eremantholide C, in the National Cancer Institute, USA (NCI, USA, against a panel of 52 human tumor cell lines of major human tumors derived from nine cancer types. Lychnopholide disclosed significant activity against 30 cell lines of seven cancer types with IC100 (total growth concentration inhibition values between 0.41 µM and 2.82 µM. Eremantholide C showed significant activity against 30 cell lines of eight cancer types with IC100 values between 21.40 µM and 53.70 µM. Lychnopholide showed values of lethal concentration 50% (LC50 for 30 human tumor cell lines between 0.72 and 10.00 µM, whereas eremantholide C presented values of LC50 for 21 human tumor cell lines between 52.50 and 91.20 µM. Lychnopholide showed an interesting profile of antitumor activity. The α-methylene-γ-lactone present in the structure of lychnopholide, besides two α,β- unsaturated carbonyl groups, might be responsible for the better activity and higher cytotoxicity of this compound in relation to eremantholide C.

  11. Hologram QSAR Studies of Antiprotozoal Activities of Sesquiterpene Lactones

    Directory of Open Access Journals (Sweden)

    Gustavo H. G. Trossini

    2014-07-01

    Full Text Available Infectious diseases such as trypanosomiasis and leishmaniasis are considered neglected tropical diseases due the lack for many years of research and development into new drug treatments besides the high incidence of mortality and the lack of current safe and effective drug therapies. Natural products such as sesquiterpene lactones have shown activity against T. brucei and L. donovani, the parasites responsible for these neglected diseases. To evaluate structure activity relationships, HQSAR models were constructed to relate a series of 40 sesquiterpene lactones (STLs with activity against T. brucei, T. cruzi, L. donovani and P. falciparum and also with their cytotoxicity. All constructed models showed good internal (leave-one-out q2 values ranging from 0.637 to 0.775 and external validation coefficients (r2test values ranging from 0.653 to 0.944. From HQSAR contribution maps, several differences between the most and least potent compounds were found. The fragment contribution of PLS-generated models confirmed the results of previous QSAR studies that the presence of α,β-unsatured carbonyl groups is fundamental to biological activity. QSAR models for the activity of these compounds against T. cruzi, L. donovani and P. falciparum are reported here for the first time. The constructed HQSAR models are suitable to predict the activity of untested STLs.

  12. Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes

    Directory of Open Access Journals (Sweden)

    Ro Dae-Kyun

    2009-07-01

    Full Text Available Abstract Background Sesquiterpene lactones are characteristic metabolites of Asteraceae (or Compositae which often display potent bioactivities and are sequestered in specialized organs such as laticifers, resin ducts, and trichomes. For characterization of sunflower sesquiterpene synthases we employed a simple method to isolate pure trichomes from anther appendages which facilitated the identification of these genes and investigation of their enzymatic functions and expression patterns during trichome development. Results Glandular trichomes of sunflower (Helianthus annuus L. were isolated, and their RNA was extracted to investigate the initial steps of sesquiterpene lactone biosynthesis. Reverse transcription-PCR experiments led to the identification of three sesquiterpene synthases. By combination of in vitro and in vivo characterization of sesquiterpene synthase gene products in Escherichia coli and Saccharomyces cerevisiae, respectively, two enzymes were identified as germacrene A synthases, the key enzymes of sesquiterpene lactone biosynthesis. Due to the very low in vitro activity, the third enzyme was expressed in vivo in yeast as a thioredoxin-fusion protein for functional characterization. In in vivo assays, it was identified as a multiproduct enzyme with the volatile sesquiterpene hydrocarbon δ-cadinene as one of the two main products with α-muuorlene, β-caryophyllene, α-humulene and α-copaene as minor products. The second main compound remained unidentified. For expression studies, glandular trichomes from the anther appendages of sunflower florets were isolated in particular developmental stages from the pre- to the post-secretory phase. All three sesquiterpene synthases were solely upregulated during the biosynthetically active stages of the trichomes. Expression in different aerial plant parts coincided with occurrence and maturity of trichomes. Young roots with root hairs showed expression of the sesquiterpene synthase genes

  13. 7-epizingiberene, a novel bisabolane sesquiterpene from wild tomato leaves

    International Nuclear Information System (INIS)

    Breeden, D.C.; Coates, R.M.

    1994-01-01

    A C 15 hydrocarbon isolated from the leaves of 2 wild tomato species, Lycopersicon hirsutum f glabratum PI 199381 and Lycopersicon hirsutum PI 365906, has been identified as 7 - epizingiberene (2), a diastereomer of zingiberene (1) that occurs in essential oil of ginger. The structure assignment for 2 is based upon its 1 H NMR, 13 C NMR, IR, UV, and mass spectral characteristics. All spectral data for zingiberene and epizingiberene are identical except for 9 of 15 13C NMR resonances, which establish the diastereomeric relationship of these sesquiterpenes. The 4S, 7R stereochemistry of epizingiberene was proven by dehydrogenation to (7R)- ar - curcumene (4). The opposite 7R and 7S stereochemistry of the zingiberenes implicates the probable occurrence of opposite sidechain rotations of a common (S)-bisabolyl carbocation intermediate (1OA) to allow stereoelectronically favorable hydride shifts in their respective biosyntheses from (E, E)-farnesyl diphosphate. (author)

  14. Spirocyclopropane-type sesquiterpene hydrocarbons from Schinus terebinthifolius Raddi.

    Science.gov (United States)

    Richter, Rita; von Reuss, Stephan H; König, Wilfried A

    2010-08-01

    The essential oil of Schinus terebinthifolius fruits was reinvestigated using GC and GC-MS techniques. Apart from several known compounds three sesquiterpene hydrocarbons with a carbon skeleton exhibiting the rare spiro(cyclopropane) moiety could be isolated by a combination of column chromatography and GLC. Structure assignments were carried out by NMR spectroscopy. These natural products are 9-spiro(cyclopropa)-4,4,8-trimethyl-2-methylenbicyclo[4.3.0]non-1(6)-ene (terebanene), 9-spiro(cyclopropa)-2,4,4,8-tetramethylbicyclo[4.3.0]nona-1,5-diene (teredenene), and (6R*,8R*)-9-spiro(cyclopropa)-2,4,4,8-tetramethylbicyclo[4.3.0]non-1-ene (terebinthene). Copyright 2010 Elsevier Ltd. All rights reserved.

  15. Sesquiterpene lactones and monoterpene glucosides from plant species Picris echoides

    Directory of Open Access Journals (Sweden)

    MILUTIN STEFANOVIC

    2000-11-01

    Full Text Available Investigation of the constituents of the aerial parts of domestic plant species Picris echoides afforded the sesquiterpene lactones, i.e., guaianolides jacquilenin (1, 11-epi-jacquilenin (2, achillin (3 and eudesmanolide telekin (4. The chemical indentification of the two monoterpene glucosides (–-cis-chrysanthenol-b-D-glucopyranoside (5 and its 6’-acetate 6 is also repoted. The guaianolide achillin (3 and the two monoterpene glucosides 5 and 6 were isolated for the first time from this plant species. Isolation was achieved by column chromatography and the structures were established mainly by the interpretation of their physical and spectral data, which were in agreement with those in the literature.

  16. Hepatoprotective sesquiterpenes and rutinosides from Murraya koenigii (L.) Spreng.

    Science.gov (United States)

    Ma, Qin-Ge; Wang, Yan-Gai; Liu, Wen-Min; Wei, Rong-Rui; Yang, Jian-Bo; Wang, Ai-Guo; Ji, Teng-Fei; Tian, Jin; Su, Ya-Lun

    2014-05-07

    Three new sesquiterpenes (1-3) and two new rutinosides (4 and 5) along with 17 known compounds (6-22) were isolated from the leaves of Murraya koenigii (L.) Spreng. The new compounds were elucidated as (3R,5S,6R)-3,5,6-trihydroxy-1,1,5-trimethylcyclohexyl-8-butyn-9-one (1), (8E,9R)-ethyl-7-(3S,5R,6S)-3,6-dihydroxy-1,1,5-trimethylcyclohexyl-9-hydroxybut-8-enoate (2), (3R)-3-O-β-D-glucoside-6'-D-apiose-β-ionone (3), 4-O-β-D-rutinosyl-3-methoxyphenyl-1-propanone (4), and 1-O-β-D-rutinosyl-2(R)-ethyl-1-pentanol (5) based on their spectroscopic data. Compounds 1, 4, 5, 18, and 21 (10 μM) exhibited moderate hepatoprotective activities.

  17. Sesquiterpenes from the essential oil of Curcuma wenyujin and their inhibitory effects on nitric oxide production.

    Science.gov (United States)

    Xia, Guiyang; Zhou, Li; Ma, Jianghao; Wang, Ying; Ding, Liqin; Zhao, Feng; Chen, Lixia; Qiu, Feng

    2015-06-01

    Three new sesquiterpenes including a new elemane-type sesquiterpene, 5βH-elem-1,3,7,8-tetraen-8,12-olide (1), and two new carabrane-type sesquiterpenes, 7α,11-epoxy-6α-methoxy-carabrane-4,8-dione (2) and 8,11-epidioxy-8-hydroxy-4-oxo-6-carabren (3), together with eight known sesquiterpenes (4-11) were isolated from Curcuma wenyujin Y. H. Chen et C. Ling. Their structures were elucidated based on extensive spectroscopic analyses. A possible biogenetic scheme for the related compounds was postulated. All of the isolated compounds were tested for inhibitory activity against LPS-induced nitric oxide production in RAW 264.7 macrophages. Meanwhile, preliminary structure-activity relationships for these compounds are discussed. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Bisabolane-type sesquiterpenes from the aerial parts of Lippia dulcis.

    Science.gov (United States)

    Ono, Masateru; Tsuru, Tsuyoshi; Abe, Hiroaki; Eto, Masashi; Okawa, Masafumi; Abe, Fumiko; Kinjo, Junei; Ikeda, Tsuyoshi; Nohara, Toshihiro

    2006-10-01

    Six new bisabolane-type sesquiterpenes, peroxylippidulcines A-C (3-5), peroxyepilippidulcine B (6), and epilippidulcines B (7) and C (8), have been isolated from the aerial parts of Lippia dulcis, along with two known bisabolane-type sesquiterpenes, seven known flavonoids, and a known triterpenoid. The structures of 3-8 were characterized on the basis of NMR, MS, specific rotation, and X-ray crystallographic analysis data and chemical evidence.

  19. Influence of cultivation site on sesquiterpene lactone composition of forage chicory (Cichorium intybus L.).

    Science.gov (United States)

    Foster, Joyce G; Clapham, William M; Belesky, David P; Labreveux, Maria; Hall, Marvin H; Sanderson, Matt A

    2006-03-08

    The forage potential of chicory (Cichorium intybus L.) has not been realized in southern West Virginia (WV) because ruminants are reluctant to consume the herbage. Chicory contains bitter sesquiterpene lactones that can adversely impact palatability. This study was undertaken to determine whether sesquiterpene lactone concentrations in chicory grown in southern WV differ from those in chicory grown in central Pennsylvania (PA) where chicory is grazed readily. Herbage was collected in 1997 and 1998 from cultivars Grasslands Puna (Puna), INIA le Lacerta (Lacerta), and Forage Feast established at research sites near State College, PA, and Beckley, WV. The total concentration of sesquiterpene lactones in WV-grown cultivars was 0.58% (dry matter basis) in Puna, 0.59% in Lacerta, and 0.79% in Forage Feast in 1997 and ranged from 1.03 (Lacerta) to 1.52% (Forage Feast) in 1998. In PA-grown cultivars, sesquiterpene lactones represented 0.16 (Puna), 0.18 (Lacerta), and 0.27% (Forage Feast) of the forage dry matter in 1997 and ranged from 0.32 (Lacerta) to 0.55% (Forage Feast) in 1998. Concentrations of lactucin, lactucopicrin, and total sesquiterpene lactones in Forage Feast exceeded those in the other cultivars grown at the same site. The lowest concentrations of lactucopicrin and total sesquiterpene lactones observed among WV-grown cultivars were higher (2-fold or more) than the highest concentrations present in cultivars grown the same year in PA. Mineral analyses of soils from the two cultivation sites indicate that P availability may influence sesquiterpene lactone composition of chicory herbage. Results provide a foundation for future studies of environmental effects on sesquiterpene lactone composition and palatability of chicory herbage.

  20. Lamellodysidines A and B, Sesquiterpenes Isolated from the Marine Sponge Lamellodysidea herbacea.

    Science.gov (United States)

    Torii, Masumi; Kato, Hikaru; Hitora, Yuki; Angkouw, Esther D; Mangindaan, Remy E P; de Voogd, Nicole J; Tsukamoto, Sachiko

    2017-09-22

    Four new sesquiterpenes, lamellodysidines A and B, O,O-dimethyllingshuiolide A, and 11-epi-O,O-dimethyllingshuiolide A (1-4), were obtained from the marine sponge, Lamellodysidea herbacea, collected in Indonesia. Their planar structures were elucidated by analysis of spectroscopic data. The absolute configurations of the new compounds were determined by the calculated ECD spectra. Compound 1 has a unique carbon framework, and 2 is a new nitrogenous sesquiterpene.

  1. Four new spiroaxane sesquiterpenes and one new rosenonolactone derivative from cultures of Basidiomycete Trametes versicolor.

    Science.gov (United States)

    Wang, Su-Rui; Zhang, Ling; Chen, He-Ping; Li, Zheng-Hui; Dong, Ze-Jun; Wei, Kun; Liu, Ji-Kai

    2015-09-01

    Four new spiroaxane sesquiterpenes, tramspiroins A-D (1-4), one new rosenonolactone 15,16-acetonide (5), and the known drimane sesquiterpenes isodrimenediol (6) and funatrol D (7) have been isolated from the cultures of Basidiomycete Trametes versicolor. The structures of new compounds were elucidated by means of spectroscopic methods. Compounds 1-7 were investigated for their cytotoxicities against five human cancer cell lines. Copyright © 2015 Elsevier B.V. All rights reserved.

  2. Dynamic evolution of herbivore-induced sesquiterpene biosynthesis in sorghum and related grass crops.

    Science.gov (United States)

    Zhuang, Xiaofeng; Köllner, Tobias G; Zhao, Nan; Li, Guanglin; Jiang, Yifan; Zhu, Liucun; Ma, Jianxin; Degenhardt, Jörg; Chen, Feng

    2012-01-01

    Sorghum (Sorghum bicolor) plants damaged by insects emit a blend of volatiles, predominantly sesquiterpenes, that are implicated in attracting natural enemies of the attacking insects. To characterize sesquiterpene biosynthesis in sorghum, seven terpene synthase (TPS) genes, SbTPS1 through SbTPS7, were identified based on their evolutionary relatedness to known sesquiterpene synthase genes from maize and rice. While SbTPS6 and SbTPS7 encode truncated proteins, all other TPS genes were determined to encode functional sesquiterpene synthases. Both SbTPS1 and SbTPS2 produced the major products zingiberene, β-bisabolene and β-sesquiphellandrene, but with opposite ratios of zingiberene to β-sesquiphellandrene. SbTPS3 produced (E)-α-bergamotene and (E)-β-farnesene. SbTPS4 formed (E)-β-caryophyllene as the major product. SbTPS5 produced mostly (E)-α-bergamotene and (Z)-γ-bisabolene. Based on the genome sequences of sorghum, maize and rice and the sesquiterpene synthase genes they contain, collinearity analysis identified the orthologs of sorghum sesquiterpene synthase genes, except for SbTPS4, in maize and rice. Phylogenetic analysis implied that SbTPS1, SbTPS2 and SbTPS3, which exist as tandem repeats, evolved as a consequence of local gene duplication in a lineage-specific manner. Structural modeling and site-directed mutagenesis experiments revealed that three amino acids in the active site play critical roles in defining product specificity of SbTPS1, SbTPS2, SbTPS3 and their orthologs in maize and rice. The naturally occurring functional variations of sesquiterpene synthases within and between species suggest that multiple mechanisms, including lineage-specific gene duplication, subfunctionalization, neofunctionalization and pseudogenization of duplicated genes, have all played a role in the dynamic evolution of insect-induced sesquiterpene biosynthesis in grasses. © 2011 The Authors. The Plant Journal © 2011 Blackwell Publishing Ltd.

  3. Seasonal variation of mono- and sesquiterpene emission rates of Scots pine

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    H. Hakola

    2006-01-01

    Full Text Available The seasonal variation of mono-and sesquiterpene emission rates of Scots pine was measured from April to October in 2004. The emission rates were measured daily in the afternoons with the exception of weekends. Emissions were measured from two branches; one of them was debudded in May (branch A, while the other was allowed to grow new needles (branch B. The monoterpene emission pattern remained almost constant throughout the measurement period, Δ3-carene being the dominant monoterpene (50-70% of the VOC emission. The standard monoterpene emission potential (30°C was highest during early summer in June (the average of the two branches 1.35 µg g-1h-1 and lowest during early autumn in September (the average of the two branches 0.20 µg g-1h-1. The monoterpene emission potential of branch A remained low also during October, whereas the emission potential of branch B was very high in October. The sesquiterpenes were mainly emitted during mid summer, the dominant sesquiterpene being β-caryophyllene. Branch A had a higher sesquiterpene emission potential than branch B and the emission maximum occurred concomitant with the high concentration of airborne pathogen spores suggesting a potential defensive role of the sesquiterpene emissions. The sesquiterpene emissions were well correlated with linalool and 1,8-cineol emissions, but not with monoterpenes. Sesquiterpene and 1,8-cineol emissions were equally well described by the temperature dependent and the temperature and light dependent algorithms. This is due to the saturation of the light algorithm as the measurements were always conducted during high light conditions.

  4. Seasonal variation of mono- and sesquiterpene emission rates of Scots pine

    Science.gov (United States)

    Hakola, H.; Tarvainen, V.; Bäck, J.; Ranta, H.; Bonn, B.; Rinne, J.; Kulmala, M.

    2006-03-01

    The seasonal variation of mono-and sesquiterpene emission rates of Scots pine was measured from April to October in 2004. The emission rates were measured daily in the afternoons with the exception of weekends. Emissions were measured from two branches; one of them was debudded in May (branch A), while the other was allowed to grow new needles (branch B). The monoterpene emission pattern remained almost constant throughout the measurement period, Δ3-carene being the dominant monoterpene (50-70% of the VOC emission). The standard monoterpene emission potential (30°C) was highest during early summer in June (the average of the two branches 1.35 µg g-1h-1) and lowest during early autumn in September (the average of the two branches 0.20 µg g-1h-1. The monoterpene emission potential of branch A remained low also during October, whereas the emission potential of branch B was very high in October. The sesquiterpenes were mainly emitted during mid summer, the dominant sesquiterpene being β-caryophyllene. Branch A had a higher sesquiterpene emission potential than branch B and the emission maximum occurred concomitant with the high concentration of airborne pathogen spores suggesting a potential defensive role of the sesquiterpene emissions. The sesquiterpene emissions were well correlated with linalool and 1,8-cineol emissions, but not with monoterpenes. Sesquiterpene and 1,8-cineol emissions were equally well described by the temperature dependent and the temperature and light dependent algorithms. This is due to the saturation of the light algorithm as the measurements were always conducted during high light conditions.

  5. Bioactive sesquiterpene lactones and other compounds isolated from Vernonia cinerea

    Science.gov (United States)

    Youn, Ui Joung; Miklossy, Gabriella; Chai, Xingyun; Wongwiwatthananukit, Supakit; Toyama, Onoomar; Songsak, Thanapat; Turkson, James; Chang, Leng Chee

    2014-01-01

    Four new sesquiterpene lactones, 8α-(2′Z-tigloyloxy)-hirsutinolide (1), 8α-(2′Z-tigloyloxy)-hirsutinolide-13-O-acetate (2), 8α-(4-hydroxytigloyloxy)-hirsutinolide (3), and 8α-hydroxy-13-O-tigloyl-hirsutinolide (4), along with seven known derivatives (5–11), three norisoprenoids (12–14), a flavonoid (15), and a linoleic acid derivative (16), were isolated from the chloroform partition of a methanol extract from the combined leaves and stems of Vernonia cinerea. Their structures were established by 1D and 2D NMR, UV, and MS analyses. Compounds 1–16 were evaluated for their inhibitory effects against the viability of U251MG glioblastoma and MDA-MB-231 breast cancer cells that harbour aberrantly-active STAT3, compared to normal NIH3T3 mouse fibroblasts that show no evidence of activated STAT3. Among the isolates, compounds 2 and 7 inhibited the aberrant STAT3 activity in glioblastoma or breast cancer cells. Further, compounds 7 and 8 inhibited viability of all three cell lines, compounds 2, 4, and 9 predominantly inhibited the viability of the U251MG glioblastoma cell line. PMID:24370662

  6. Sesquiterpenes from the Brazilian Red Alga Laurencia dendroidea J. Agardh

    Directory of Open Access Journals (Sweden)

    Fernanda Lacerda da Silva Machado

    2014-03-01

    Full Text Available Two new chamigrane sesquiterpenes 1–2 and three known compounds 3–5 were isolated from a lipophilic extract of the red alga Laurencia dendroidea collected from the Southeastern Brazilian coast. Dendroidone (1 and dendroidiol (2 were isolated from samples collected at Biscaia Inlet, Angra dos Reis, Rio de Janeiro and at Manguinhos Beach, Serra, Espírito Santo, respectively. Debromoelatol (3, obtusane (4 and (1S*,2S*,3S*,5S*,8S*,9S*-2,3,5,9-tetramethyltricyclo[6.3.0.01.5]undecan-2-ol (5 were obtained from specimens collected at Vermelha Beach, Parati, Rio de Janeiro. The structures of new compounds were elucidated by extensive NMR (1H-, 13C-, COSY, HSQC, HMBC and NOESY and high resolution mass spectrometry analysis. Additionally, the absolute configuration of compound 2 was assigned by X-ray analysis. Full spectroscopic data is described for the first time for compound 3. Anti-inflammatory and antimycobacterial activities of compounds 2–5 were evaluated. Compounds 3–5 inhibited the release of inflammatory mediator NO while TNF-α levels were only affected by 3. All compounds tested displayed moderate antimycobacterial action.

  7. Quantitative Structure ‒ Antiprotozoal Activity Relationships of Sesquiterpene Lactones

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    Reto Brun

    2009-06-01

    Full Text Available Prompted by results of our previous studies where we found high activity of some sesquiterpene lactones (STLs against Trypanosoma brucei rhodesiense (which causes East African sleeping sickness, we have now conducted a structure-(in-vitro-activity study on a set of 40 STLs against T. brucei rhodesiense, T. cruzi, Leishmania donovani and Plasmodium falciparum. Furthermore, cytotoxic activity against L6 rat skeletal myoblast cells was assessed. Some of the compounds possess high activity, especially against T. brucei (e.g. helenalin and some of its esters with IC50-values of 0.05-0.1 µM, which is about 10 times lower than their cytotoxic activity. It was found that all investigated antiprotozoal activities are significantly correlated with cytotoxicity and the major determinants for activity are a,b-unsaturated structural elements, also known to be essential for other biological activities of STLs. It was observed, however, that certain compounds are considerably more toxic against protozoa than against mammalian cells while others are more cytotoxic than active against the protozoa. A comparative QSAR analysis was therefore undertaken, in order to discern the antiparasitic activity of STLs against T. brucei and cytotoxicity. Both activities were found to depend to a large extent on the same structural elements and molecular properties. The observed variance in the biological data can be explained in terms of subtle variations in the relative influences of various molecular descriptors.

  8. Sesquiterpene amino ether and cytotoxic phenols from Dendrobium wardianum Warner.

    Science.gov (United States)

    Zhang, Cong; Liu, Shou-Jin; Yang, Liu; Yuan, Ming-Yan; Li, Jin-Yu; Hou, Bo; Li, Hong-Mei; Yang, Xing-Zhi; Ding, Chang-Chun; Hu, Jiang-Miao

    2017-10-01

    A new bibenzyl derivative, dendrocandin V (1) and a new sesquiterpene amino ether, wardianumine A (2), together with eleven known compounds, including phenanthrenes (denbinobin (3), 9,10-dihydro-denbinobin (4), mostatin (5), loddigesiinols A (6)), bibenzyls (moscatilin (7), 5-hydroxy-3,4'-dimethoxybibenzyl (8), 3,4-dihydroxy-5,4'-dimethoxy bibenzyl (9), dendrocandin A (10), gigantol (11), dendrocandin U (12)) and an alkaloids (dihydroshihunine, 13) were isolated from the EtOH extraction of stems of Dendrobium wardianum Warner. Isolation of the new compound 2 indicated that N,N-dimethylethanolamine as the key adduction in the synthesis of dendroxine and its analogs in Dendrobium species. The hypothetical biosynthetic pathway of 2 was then postulated. Inspired by literature and traditional usage of the herbal medicine, some compounds were sent for cytotoxic activity and the results indicated that compounds 1, 3, 4, 5 showed cytotoxic activities against five human cancer cell lines (HL-60, A-549, SMMC-7721, MCF-7, and SW-480) with IC50 from 2.33-38.48μM. Among those compounds, 3 and 4 showed cell line selectivity with strong activity comparable to DDP. Copyright © 2017. Published by Elsevier B.V.

  9. Antibacterial Activity of Germacrane Type Sesquiterpenes from Curcuma heyneana Rhizomes

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    Hartiwi Diastuti

    2014-03-01

    Full Text Available The isolation of terpenoids from C. heyneana rhizomes and their antibacterial activity have been conducted. The terpenoids were isolated by using vacuum liquid chromatography and radial chromatography. The structures of the compounds were determined based on spectroscopic data (1H-NMR, 13C-NMR (1D and 2D. The antibacterial activity was carried out by using microdilution method and evaluated against eight bacteria. Three germacrane type sesquiterpenes have been isolated from C. heyneana rhizhomes and were identified as germacrone, dehydrocurdione, and 1(10,4(5-diepoxygermacrone. Germacrone showed highest antibacterial activity against P. aeruginosa with MIC values of 15.6 µg/mL and MBC values 31.2 µg/mL. Dehydrocurdione showed highest antibacterial activity against B. subtilis with MIC values of 31.2 µg/mL and MBC values of 31.2 µg/mL. However, 1(10,4(5-diepoxygermacrone showed a weak antibacterial activity.

  10. New sesquiterpene acid and inositol derivatives from Inula montana L.

    Science.gov (United States)

    Garayev, Elnur; Herbette, Gaëtan; Di Giorgio, Carole; Chiffolleau, Philippe; Roux, David; Sallanon, Huguette; Ollivier, Evelyne; Elias, Riad; Baghdikian, Béatrice

    2017-07-01

    A phytochemical investigation of the ethanol extract of leaves and flowers of Inula montana L. led to the isolation of one new sesquiterpene acid called Eldarin (1) and four new inositol derivatives, Myoinositol,1,5-diangelate-4,6-diacetate (2), Myoinositol,1,6-diangelate-4,5-diacetate (3), Myoinositol-1-angelate-4,5-diacetate-6-(2-methylbutirate) (4), Myoinositol-1-angelate-4,5-diacetate-6-isovalerate (5) isolated for the first time, along with eleven known compounds described for the first time in Inula montana, 1β-Hydroxyarbusculin A (6), Artemorin (7), Santamarin (8), Chrysosplenol C (9), 6-Hydroxykaempferol 3,7-dimethyl ether (10), Reynosin (11), Calenduladiol-3-palmitate (12), Costunolide (13), 4-Hydroxy-3,5-dimethoxybenzenemethanol (14), 9β-Hydroxycostunolide (15) and Hispidulin (16). Structural elucidation has been carried out by spectral methods, such as 1D and 2D NMR, IR, UV and HR-ESI-MS. These compounds have been tested in vitro for anti-inflammatory and cytotoxic activity on macrophages RAW 264.7. As a result, compounds 2, 3, 7, 13, 14, 15 and 16 showed a release of NO with IC 50 value <30μM on macrophages. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. In vitro analysis of the anthelmintic activity of forage chicory (Cichorium intybus L.) sesquiterpene lactones against a predominatly Haemonchus contortus egg population

    Science.gov (United States)

    The anthelmintic activity of chicory (Cichorium intybus L.) herbage has been attributed to sesquiterpene lactones. Chicory leaves contain significant amounts of lactucin (LAC), 8-deoxylactucin (DOL), and lactucopicrin (LPIC), but the proportions of these three sesquiterpene lactones vary among fora...

  12. Tricyclic sesquiterpene copaene prevents H2O2-induced neurotoxicity

    Directory of Open Access Journals (Sweden)

    Hasan Turkez

    2014-02-01

    Full Text Available Aim: Copaene (COP, a tricyclic sesquiterpene, is present in several essential oils of medicinal and aromatic plants and has antioxidant and anticarcinogenic features. But, very little information is known about the effects of COP on oxidative stress induced neurotoxicity. Method: We used hydrogen peroxide (H2O2 exposure for 6 h to model oxidative stress. Therefore, this experimental design allowed us to explore the neuroprotective potential of COP in H2O2-induced toxicity in rat cerebral cortex cell cultures for the first time. For this purpose, methyl thiazolyl tetrazolium (MTT and lactate dehydrogenase (LDH release assays were carried out to evaluate cytotoxicity. Total antioxidant capacity (TAC and total oxidative stress (TOS parameters were used to evaluate oxidative changes. In addition to determining of 8-hydroxy-2-deoxyguanosine (8-OH-dG levels, the single cell gel electrophoresis (SCGE or comet assay was also performed for measuring the resistance of neuronal DNA to H2O2-induced challenge. Result: The results of this study showed that survival and TAC levels of the cells decreased, while TOS, 8-OH-dG levels and the mean values of the total scores of cells showing DNA damage increased in the H2O2 alone treated cultures. But pre-treatment of COP suppressed the cytotoxicity, genotoxicity and oxidative stress which were increased by H2O2. Conclusion: It is proposed that COP as a natural product with an antioxidant capacity in mitigating oxidative injuries in the field of neurodegenerative diseases. [J Intercult Ethnopharmacol 2014; 3(1.000: 21-28

  13. Unusual metal complex of cadinane sesquiterpene alkaloid and new neolignan glycosides from Alangium alpinum.

    Science.gov (United States)

    Ren, Jie; Xie, Yang-Guo; Guo, Yi-Gong; Zhu, Sheng-Lan; Jin, Hui-Zi; Zhang, Wei-Dong

    2018-03-01

    One unusual metal complex of cadinane sesquiterpene alkaloid (1), one new cadinane sesquiterpene alkaloid (2) and two new neolignan glycosides (3-4) along with six known cadinane sesquiterpene derivatives (5-10), nineteen known phenolic glycosides (11-29) were isolated from the aerial parts of Alangium alpinum. Structures of new crystals of metal complex were characterized by X-Ray diffraction and ICP-AES analysis. Other new compounds were elucidated by combined use and detailed analysis of HR-ESIMS, 1D and 2D NMR and CD spectroscopic method. In addition, all isolated compounds were tested for their inhibitory effects against TNF-α induced NF-κB activation in Hela cells and NO production in RAW 264.7 macrophages. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. New sesquiterpene lactones from sunflower root exudate as germination stimulants for Orobanche cumana.

    Science.gov (United States)

    Raupp, Frank M; Spring, Otmar

    2013-11-06

    Orobanche cumana is a serious threat for cultivation of sunflower in Europe and Asia. Germination of the parasite is induced by metabolites released from the host root system. The first germination stimulant from sunflower root exudate was recently identified as dehydrocostus lactone, a sesquiterpene lactone. Bioassay-guided fractionation of root exudates now showed the release of additional sesquiterpene lactones. Besides dehydrocostus lactone, costunolide, tomentosin, and 8-epixanthatin were purified and identified spectroscopically. All four compounds induced germination of O. cumana at nano- to micromolar concentrations. Costunolide and dehydrocostus lactone concentrations above 1 μM reduced the activity, and application of 100 μM inhibited germination irreversibly. Seeds of Phelipanche ramosa could not be induced with costunolide. O. cumana seeds also germinated with GR24, a synthetic strigolactone. No bioactive fraction of sunflower contained compounds of this type. This supports previous findings that sesquiterpene lactones instead of strigolactones trigger the sunflower/O. cumana interaction.

  15. Root colonization by symbiotic arbuscular mycorrhizal fungi increases sesquiterpenic acid concentrations in Valeriana officinalis L.

    Science.gov (United States)

    Nell, Monika; Wawrosch, Christoph; Steinkellner, Siegrid; Vierheilig, Horst; Kopp, Brigitte; Lössl, Andreas; Franz, Chlodwig; Novak, Johannes; Zitterl-Eglseer, Karin

    2010-03-01

    In some medicinal plants a specific plant-fungus association, known as arbuscular mycorrhizal (AM) symbiosis, increases the levels of secondary plant metabolites and/or plant growth. In this study, the effects of three different AM treatments on biomass and sesquiterpenic acid concentrations in two IN VITRO propagated genotypes of valerian ( VALERIANA OFFICINALIS L., Valerianaceae) were investigated. Valerenic, acetoxyvalerenic and hydroxyvalerenic acid levels were analyzed in the rhizome and in two root fractions. Two of the AM treatments significantly increased the levels of sesquiterpenic acids in the underground parts of valerian. These treatments, however, influenced the biomass of rhizomes and roots negatively. Therefore this observed increase was not accompanied by an increase in yield of sesquiterpenic acids per plant. Furthermore, one of the two genotypes had remarkably high hydroxyvalerenic acid contents and can be regarded as a hydroxyvalerenic acid chemotype. Copyright Georg Thieme Verlag KG Stuttgart New York.

  16. Anti-inflammatory sesquiterpene lactones from Onopordum illyricum L. (Asteraceae), an Italian medicinal plant.

    Science.gov (United States)

    Formisano, Carmen; Sanna, Cinzia; Ballero, Mauro; Chianese, Giuseppina; Sirignano, Carmina; Rigano, Daniela; Millán, Estrella; Muñoz, Eduardo; Taglialatela-Scafati, Orazio

    2017-01-01

    Onopordum illyricum L. is a medicinal plant used in the Mediterranean area as antipyretic for the treatment of respiratory and urinary inflammations and to treat skin ulcers. Repeated chromatographic purification of O. illyricum aerial parts led to the isolation of six known sesquiterpenes, which were evaluated for the inhibition of the pro-inflammatory transcription factors NF-κB and STAT3 and for the activation of the transcription factor Nrf2, which regulates the cellular antioxidant response. Structure-activity relationships were interpreted by the NMR-based cysteamine assay. The sesquiterpene lactone vernomelitensin significantly inhibited NF-κB and STAT3, showing also a significant Nrf2 activation. Accordingly, the cysteamine assay selected vernomelitensin as the most reactive of the isolated sesquiterpenes, identifying the α,β-unsaturated aldehyde moiety as responsible for the higher (re)activity. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Sesquiterpene and Acetogenin Derivatives from the Marine Red Alga Laurencia okamurai

    Directory of Open Access Journals (Sweden)

    Bin-Gui Wang

    2012-12-01

    Full Text Available In addition to 13 known compounds, four new bisabolane sesquiterpenes, okamurenes A–D (1–4, a new chamigrane derivative, okamurene E (5, and a new C12-acetogenin, okamuragenin (6, were isolated from the marine red alga Laurencia okamurai. The structures of these compounds were determined through detailed spectroscopic analyses. Of these, okamurenes A and B (1 and 2 are the first examples of bromobisabolane sesquiterpenes possessing a phenyl moiety among Laurencia-derived sesquiterpenes, while okamuragenin (6 was the first acetogenin aldehyde possessing a C12-carbon skeleton. Each of the isolated compounds was evaluated for the brine shrimp (Artemia salina lethal assay and 7-hydroxylaurene displayed potent lethality with LD50 1.8 μM.

  18. Compartmentation of mono- and sesqui-terpene biosynthesis of the essential oil in poncirus trifoliata

    International Nuclear Information System (INIS)

    Heinrich, G.; Wegener, R.; Schultze, W.

    1980-01-01

    The fruit of Poncirus trifoliata shows glandular cells complexes in the exocarp, which produce a volatile oil rich in monoterpenes but poor in sesquiterpenes and oxigenated compounds. The juice vesicles of the endocarp possess similar cell complexes mainly containing sesquiterpenes and oxigenated compounds, whereas monoterpenes only occur in small amounts. By the use of combined gas chromatography-mass spectrometry 19 components of the rind oil and 15 compounds of the endocarp oil could be identified. As demonstrated by electron microscopy the terpenes most probably are synthesized predominantly, if not exclusively in plastids. As shown by gasradiochromatography radioactive precursors ( 14 Co 2 and 14 C-leucine) are incorporated into mono- and sesqui-terpenes to a different extent. This is due to two gland types producing essential oils of different composition with regard to their mono- and sesqui-terpene percentage. In fruit development the exocarp glands differentiate earlier than the endocarp glands do. The activity of exogenously applied 14 Co 2 first reaches the peripheral glands and later on appears in the interior glands. Depending upon the growth season, labelled leucine transported by the conducting tissues from lower plant parts leads to a high specific activity of the sesqui-terpenes and oxigenated compounds. It could be argued that in this instance the glands of the pulp are better provided with precursors than the exocarp glands. The successive maxima of essential oil production in both glandular complexes, and the changes in the concentration of individual oil constituents during the ontogeny of the fruit also contribute to different incorporation ratios of radioactive precursors into mono- and sesqui-terpenes. (author)

  19. Antibacterial and cytotoxic activities of the sesquiterpene lactones cnicin and onopordopicrin.

    Science.gov (United States)

    Bach, Sandra M; Fortuna, Mario A; Attarian, Rodgoun; de Trimarco, Juliana T; Catalán, César A N; Av-Gay, Yossef; Bach, Horacio

    2011-02-01

    The antimicrobial and cytotoxic activities of chloroform extracts from the weeds Centaurea tweediei and C. diffusa, and the main sesquiterpene lactones isolated from these species, onopordopicrin and cnicin, respectively, were assayed. Results show that the chloroform extracts from both Centaurea species possess antibacterial activities against a panel of Gram-positive and Gram-negative bacteria. Remarkable antibacterial activity against methicillin-resistant Staphylococcus aureus was also measured. Both the extracts and the purified sesquiterpene lactones show high cytotoxicity against human-derived macrophages. Despite this cytotoxicity, C. diffusa chloroform extract and cnicin are attractive candidates for evaluation as antibiotics in topical preparations against skin-associated pathogens.

  20. A new eremophilane-type sesquiterpene from the phytopatogen fungus Lasiodiplodia theobromae (Sphaeropsidaceae)

    International Nuclear Information System (INIS)

    Nunes, Fatima M.; Oliveira, Maria da Conceicao F. de; Arriaga, Angela M.C.; Lemos, Telma L.G.; Andrade-Neto, Manoel; Mattos, Marcos C. de; Mafezoli, Jair; Viana, Francisco M.P.; Ferreira, Viviane M.; Rodrigues-Filho, Edson; Ferreira, Antonio G.

    2008-01-01

    The phytopatogenic fungus Lasiodiplodia theobromae, isolated from guava, was cultivated in rice for 32 days at room temperature. Extraction with CH 2 Cl 2 :MeOH (3:7), followed by chromatography fractionation of the extract provided ergosterol. From the fungus culture in Czapeck medium for 40 days at room temperature, were isolated isocoumarin cis-4-hydroxymeleine and an eremophilane-type sesquiterpene. The latter compound is being reported for the first time in the literature. Also, this is the first time that an eremophilane sesquiterpene is described for Lasiodiplodia genus. (author)

  1. Cloning, expression, and characterization of epi-cedrol synthase, a sesquiterpene cyclase from Artemisia annua L.

    Science.gov (United States)

    Mercke, P; Crock, J; Croteau, R; Brodelius, P E

    1999-09-15

    Sesquiterpene cyclases (synthases) catalyze the conversion of the isoprenoid intermediate farnesyl diphosphate to various sesquiterpene structural types. In plants, many sesquiterpenes are produced as defensive chemicals (phytoalexins) or mediators of chemical communication (i.e., pollinator attractants). A number of sesquiterpene synthases are present in Artemisia annua L. (annual wormwood). We have isolated a cDNA clone encoding one of these, epi-cedrol synthase. This clone contains a 1641-bp open reading frame coding for 547 amino acids (63.5 kDa), a 38-bp 5'-untranslated end, and a 272-bp 3'-untranslated sequence. The deduced amino acid sequence was 32 to 43% identical with the sequences of other known sesquiterpene cyclases from angiosperms. When expressed in Escherichia coli, the recombinant enzyme catalyzed the formation of both olefinic (3%) and oxygenated (97%) sesquiterpenes from farnesyl diphosphate. GC-MS analysis identified the olefins as alpha-cedrene (57% of the olefins), beta-cedrene (13%), (E)-beta-farnesene (5%), alpha-acoradiene (1%), (E)-alpha-bisabolene (8%), and three unknown olefins (16%) and the oxygenated sesquiterpenes (97% of total sesquiterpene generated, exclusive of farnesol and nerolidol) as cedrol (4%) and epi-cedrol (96%). epi-Cedrol synthase was not active with geranylgeranyl diphosphate as substrate, whereas geranyl diphosphate was converted to monoterpenes by the recombinant enzyme at a rate of about 15% of that observed with farnesyl diphosphate as substrate. The monoterpene olefin products are limonene (45%), terpinolene (42%), gamma-terpinene (8%), myrcene (5%), and alpha-terpinene (2%); a small amount of the monoterpene alcohol terpinen-4-ol is also produced. The pH optimum for the recombinant enzyme is 8.5-9.0 (with farnesyl diphosphate as substrate) and the K(m) values for farnesyl diphosphate are 0.4 and 1.3 microM at pH 7. 0 and 9.0, respectively. The K(m) for Mg(2+) is 80 microM at pH 7.0 and 9.0.

  2. A new eremophilane-type sesquiterpene from the phytopatogen fungus Lasiodiplodia theobromae (Sphaeropsidaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Nunes, Fatima M.; Oliveira, Maria da Conceicao F. de; Arriaga, Angela M.C.; Lemos, Telma L.G.; Andrade-Neto, Manoel; Mattos, Marcos C. de [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica]. E-mail: mcfo@ufc.br; Mafezoli, Jair [Universidade de Fortaleza, CE (Brazil). Curso de Farmacia; Viana, Francisco M.P.; Ferreira, Viviane M. [EMBRAPA Agroindustria Tropical, Fortaleza, CE (Brazil). Lab. de Fitopatologia; Rodrigues-Filho, Edson; Ferreira, Antonio G. [Universidade Federal de Sa Carlos (UFSCAR), SP (Brazil). Dept. de Quimica

    2008-07-01

    The phytopatogenic fungus Lasiodiplodia theobromae, isolated from guava, was cultivated in rice for 32 days at room temperature. Extraction with CH{sub 2}Cl{sub 2}:MeOH (3:7), followed by chromatography fractionation of the extract provided ergosterol. From the fungus culture in Czapeck medium for 40 days at room temperature, were isolated isocoumarin cis-4-hydroxymeleine and an eremophilane-type sesquiterpene. The latter compound is being reported for the first time in the literature. Also, this is the first time that an eremophilane sesquiterpene is described for Lasiodiplodia genus. (author)

  3. Neolignans and sesquiterpenes from leaves and embryogenic cultures of Ocotea Catharinensis (Lauraceae)

    International Nuclear Information System (INIS)

    Funasaki, Mariko; Kato, Massuo J.

    2009-01-01

    The extracts from leaves of Ocotea catharinensis Mez (Lauraceae) were found to contain fourteen neolignans and two sesquiterpenes: nine benzofuran types (including three new compounds 1e, 2f and 4b), one new seco-benzofuran type (3b), two bicyclo[3.2.1]octane types (including the new compound 5c), two new dimers of bicyclo[3.2.1]octane type (7a and 7b) and two sesquiterpenes (including a new humulanol 9). In addition, seven neolignans were also showed to occur in somatic embryos of O. catharinensis including one new bicyclo[3.2.1]octane type (4a). (author)

  4. Neolignans and sesquiterpenes from leaves and embryogenic cultures of Ocotea Catharinensis (Lauraceae)

    Energy Technology Data Exchange (ETDEWEB)

    Funasaki, Mariko; Kato, Massuo J. [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Quimica; Lordello, Ana Luisa L. [Universidade Federal do Parana (UFPR), Curitiba, PR (Brazil). Dept. de Quimica; Viana, Ana Maria [Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC (Brazil). Centro de Ciencias Biologicas. Dept. de Botanica; Santa-Catarina, Claudete; Floh, Eny I.S. [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Biociencias; Yoshida, Massayoshi [Centro de Biotecnologia da Amazonia, Manaus, AM (Brazil)

    2009-07-01

    The extracts from leaves of Ocotea catharinensis Mez (Lauraceae) were found to contain fourteen neolignans and two sesquiterpenes: nine benzofuran types (including three new compounds 1e, 2f and 4b), one new seco-benzofuran type (3b), two bicyclo[3.2.1]octane types (including the new compound 5c), two new dimers of bicyclo[3.2.1]octane type (7a and 7b) and two sesquiterpenes (including a new humulanol 9). In addition, seven neolignans were also showed to occur in somatic embryos of O. catharinensis including one new bicyclo[3.2.1]octane type (4a). (author)

  5. Anthelmintic potential of chicory forage is influenced by sesquiterpene lactone composition

    Science.gov (United States)

    The anthelmintic activity of chicory (Cichorium intybus L.) herbage has been attributed to sesquiterpene lactones (SLs). Chicory leaves contain lactucin (LAC), 8-deoxylactucin (DOL), and lactucopicrin (LPIC), but the amounts of these three SLs vary among cultivars. The objective of this study was...

  6. Antimalarial activity of lactucin and lactucopicrin: sesquiterpene lactones isolated from Cichorium intybus L.

    Science.gov (United States)

    Bischoff, Theodore A; Kelley, Charles J; Karchesy, Yvette; Laurantos, Maria; Nguyen-Dinh, Phuc; Arefi, Abdul Ghafoor

    2004-12-01

    Folklore reports from Afghanistan prior to the wars described the use of aqueous root extracts of Cichorium intybus (L.) as a light-sensitive plant remedy for malaria. Preparative isolation and bioassay against HB3 clone of strain Honduras-1 of Plasmodium falciparum identified the previously known light-sensitive sesquiterpene lactones Lactucin and Lactucopicrin to be antimalarial compounds.

  7. Sesquiterpene Lactones from Artemisia Genus: Biological Activities and Methods of Analysis

    Directory of Open Access Journals (Sweden)

    Bianca Ivanescu

    2015-01-01

    Full Text Available Sesquiterpene lactones are a large group of natural compounds, found primarily in plants of Asteraceae family, with over 5000 structures reported to date. Within this family, genus Artemisia is very well represented, having approximately 500 species characterized by the presence of eudesmanolides and guaianolides, especially highly oxygenated ones, and rarely of germacranolides. Sesquiterpene lactones exhibit a wide range of biological activities, such as antitumor, anti-inflammatory, analgesic, antiulcer, antibacterial, antifungal, antiviral, antiparasitic, and insect deterrent. Many of the biological activities are attributed to the α-methylene-γ-lactone group in their molecule which reacts through a Michael-addition with free sulfhydryl or amino groups in proteins and alkylates them. Due to the fact that most sesquiterpene lactones are thermolabile, less volatile compounds, they present no specific chromophores in the molecule and are sensitive to acidic and basic mediums, and their identification and quantification represent a difficult task for the analyst. Another problematic aspect is represented by the complexity of vegetal samples, which may contain compounds that can interfere with the analysis. Therefore, this paper proposes an overview of the methods used for the identification and quantification of sesquiterpene lactones found in Artemisia genus, as well as the optimal conditions for their extraction and separation.

  8. Trilobolide and related sesquiterpene lactones from Laser trilobum possessing immunobiological properties

    Czech Academy of Sciences Publication Activity Database

    Harmatha, Juraj; Buděšínský, Miloš; Vokáč, Karel; Kostecká, Petra; Kmoníčková, Eva; Zídek, Zdeněk

    2013-01-01

    Roč. 89, September (2013), s. 157-166 ISSN 0367-326X R&D Projects: GA ČR GA305/07/0061 Institutional support: RVO:61388963 ; RVO:68378041 Keywords : Laser trilobum * nitric oxide * trilobolide * thapsigargin * cytokine * sesquiterpene Subject RIV: CC - Organic Chemistry; FR - Pharmacology ; Medidal Chemistry (UEM-P) Impact factor: 2.216, year: 2013

  9. Sesquiterpene lactone trilobolide activates production of interferon-γ and nitric oxide

    Czech Academy of Sciences Publication Activity Database

    Kmoníčková, Eva; Harmatha, Juraj; Vokáč, Karel; Kostecká, Petra; Farghali, H.; Zídek, Zdeněk

    2010-01-01

    Roč. 81, č. 8 (2010), s. 1213-1219 ISSN 0367-326X R&D Projects: GA ČR GA305/07/0061 Institutional research plan: CEZ:AV0Z50390512; CEZ:AV0Z40550506 Keywords : trilobolide * nitric oxide * sesquiterpene lactones Subject RIV: FR - Pharmacology ; Medidal Chemistry Impact factor: 1.899, year: 2010

  10. Immunobiological properties of sesquiterpene lactones obtained by chemically transformed structural modifications of trilobolide

    Czech Academy of Sciences Publication Activity Database

    Harmatha, Juraj; Vokáč, Karel; Buděšínský, Miloš; Zídek, Zdeněk; Kmoníčková, Eva

    2015-01-01

    Roč. 107, Dec (2015), s. 90-99 ISSN 0367-326X R&D Projects: GA ČR GA305/07/0061 Institutional support: RVO:61388963 ; RVO:68378041 Keywords : sesquiterpene lactones * guaianolides * trilobolide derivatives * nitric oxide * cytokines * immunoactivity Subject RIV: CC - Organic Chemistry Impact factor: 2.408, year: 2015

  11. Cytotoxic Properties of Selected Sesquiterpene Alcohols on Human Cervix Carcinoma Cell Lines

    Czech Academy of Sciences Publication Activity Database

    Ryabchenko, B.; Tulupová, Elena; Schmidt, E.; Jaeger, W.; Jirovetz, L.

    2011-01-01

    Roč. 14, č. 3 (2011), s. 316-319 ISSN 0972-060X Institutional support: RVO:68378041 Keywords : sesquiterpene alcohols * actinomycin D * cis-nerolidol Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.290, year: 2011

  12. Structure-cytotoxicity relationships of some helenanolide-type sesquiterpene lactones

    NARCIS (Netherlands)

    Beekman, AC; Woerdenbag, HJ; vanUden, W; Pras, N; Konings, AWT; Wikstrom, HV; Schmidt, TJ

    This study deals with the cytotoxicity of helenanolide-type (10 alpha-methylpseudoguaianolide) sesquiterpene lactones. We determined the influence of substitution patterns on the toxicity of 21 helenanolides to a cloned Ehrlich ascites tumor cell line, EN2. Within a series of helenalin esters, the

  13. Enhancing sesquiterpene production in Saccharomyces cerevisiae through in silico driven metabolic engineering

    DEFF Research Database (Denmark)

    Asadollahi, Mohammadali; Maury, Jerome; Patil, Kiran Raosaheb

    2009-01-01

    A genome-scale metabolic model was used to identify new target genes for enhanced biosynthesis of sesquiterpenes in the yeast Saccharomyces cerevisiae. The effect of gene deletions on the flux distributions in the metabolic model of S. cerevisiae was assessed using OptGene as the modeling framewo...

  14. New diastereomeric bis-sesquiterpenes from Hainan marine sponges Axinyssa variabilis and Lipastrotethya ana

    NARCIS (Netherlands)

    Mao, S.-C.; Manzo, E.; Guo, Y.-W.; Gavagnin, M.; Mollo, E.; Ciavatta, M.L.; van Soest, R.; Cimino, G.

    2007-01-01

    Three unprecedented diastereoisomeric dimers, cis-dimer A, cis-dimer B and trans-dimer C, exhibiting a bis-bisabolene skeleton, and a new sesquiterpene, dehydrotheonelline, their potential precursor, have been isolated from the South China Sea sponges Axinyssa variabilis and Lipastrotethya ana,

  15. Puupehanol, a Sesquiterpene-Dihydroquinone Derivative from the Marine Sponge Hyrtios sp

    Science.gov (United States)

    Puupehanol (1), a new sesquiterpene-dihydroquinone derivative, was isolated from the marine sponge Hyrtios sp., along with the known compounds puupehenone (2) and chloropuupehenone (3). The structure of 1 was established as (20R,21R)-21-hydroxy-20,21-dihydropuupehenone by interpretation of spectros...

  16. Two new sesquiterpene coumarins, ferusinol and samarcandin diastereomer from Ferula Sinaica

    International Nuclear Information System (INIS)

    Gohar, Ahmad A.; El-Bassuony, Ashraf A.

    2007-01-01

    Re-investigation of the CH2Cl2: CH3OH (1:1) extracts of the roots of Ferula Sinaica resulted in the isolation of ferusinol, a new sesquiterpene coumarin with a rare carbon skeleton and samarcandin diastereomer. The structures of the isolated compounds were determined by MS, H, C-1D and 2D NMR spectral analysis. (author)

  17. Three New Sesquiterpene Aryl Esters from the Mycelium of Armillaria mellea.

    Science.gov (United States)

    Chen, Chien-Chih; Kuo, Yueh-Hsiung; Cheng, Jing-Jy; Sung, Ping-Jyun; Ni, Ching-Li; Chen, Chin-Chu; Shen, Chien-Chang

    2015-05-29

    Three new sesquiterpene aryl esters and eight known compounds were isolated from the EtOH extract of the mycelium of Armillaria mellea. The structures of new compounds were established by analysis of their spectroscopic data. Some of the isolates showed cytotoxicity to a variety of cancer cell lines, including MCF-7, H460, HT-29, and CEM.

  18. Three New Sesquiterpene Aryl Esters from the Mycelium of Armillaria mellea

    Directory of Open Access Journals (Sweden)

    Chien-Chih Chen

    2015-05-01

    Full Text Available Three new sesquiterpene aryl esters and eight known compounds were isolated from the EtOH extract of the mycelium of Armillaria mellea. The structures of new compounds were established by analysis of their spectroscopic data. Some of the isolates showed cytotoxicity to a variety of cancer cell lines, including MCF-7, H460, HT-29, and CEM.

  19. Two new guaiane sesquiterpene lactones from the aerial parts of Artemisia vulgaris.

    Science.gov (United States)

    Hanh, Tran Thi Hong; Hang, Le Thi Thuy; Huong, Phan Thi Thanh; Trung, Nguyen Quang; Cuong, Tran Van; Thanh, Nguyen Van; Cuong, Nguyen Xuan; Nam, Nguyen Hoai; Minh, Chau Van

    2017-09-25

    Two new guaiane sesquiterpene lactones, vulgarolides A and B (1 and 2), were isolated from Artemisia vulgaris aerial parts using various chromatographic separations. The structure elucidation was performed by combination of spectroscopic experiments including 1D and 2D NMR, HR ESI MS, and CD. Their in vitro cytotoxic activities against five human cancer cell lines were also evaluated using SRB method.

  20. Mountain birch – potentially large source of sesquiterpenes into high latitude atmosphere

    Directory of Open Access Journals (Sweden)

    A. Arneth

    2009-11-01

    Full Text Available Emissions of volatile organic compounds (VOCs from mountain birches were measured in Abisko, northern Sweden. Mountain birches make up the majority of the tree biomass in Scandinavian high latitudes, a region subject to significant climate warming. The measurements were carried out in two growing seasons. The emissions of four branches, each from a different individual tree, were measured in June–August 2006 and one of them again in July 2007. The measurements were conducted using a dynamic flow through chamber covered with Teflon film. The studied mountain birches were found to emit substantial amounts of linalool, monoterpenes and sesquiterpenes. The monoterpene emission was dominated by sabinene. The magnitude and composition of the sesquiterpene emission changed dramatically between the years. For example, the average α-farnesene emission potential in 2006 was almost 2600 ng gdw−1 h−1 (3.5 pmol gdw−1 s−1 while in 2007 α-farnesene was not detected at all. Also the emissions of other sesquiterpenes decreased in 2007 to a fraction of that in 2006. One possible explanation for the change in emissions is the herbivory damage that occurred in the area in 2004. Herbivory is known to enhance the emissions of sesquiterpenes, especially those of α-farnesene, and the effect may last for several years.

  1. Biogenic Trypanocidal Sesquiterpenes: Lead Compounds to Design Future Trypanocidal Drugs - A Mini Review

    Directory of Open Access Journals (Sweden)

    Soodabeh Saeidnia

    2013-05-01

    Full Text Available Human trypanosomiasis is a parasitic disease among poor people in Africa and Latin America. Therapy against African and American trypanosomiasis is based on a few drugs that often cause severe side-effects. Therefore, it is essential to develop drug discovery especially from natural origins. Sesquiterpenes, a diverse group of natural terpenoids, are found in essential oils of many plants and show a broad range of bioactivities. They act through multiple mechanisms in the chemotherapy of trypanosomiasis. Some of these active compounds contain hydroperoxides, aldehydes, alcohols, α,β-unsaturated γ-lactone and even halogenated moieties. Among the compounds reported, sesquiterpene lactones showed a potent anti-trypanosoma effect comparable with commercial trypanocidal drugs. Trypanocidal activity of sesquiterpene lactones mostly depends on the reaction between γ-lactone moieties and nucleophile groups of trypanithione, which is essential for Trypanosoma defense against the oxidative stresses. Elatol is a sesquiterpenoid from marine algae, with a different structure and considerable trypanocidal activity which could be an interesting candidate for further antiprotozoal investigations. To develop novel drugs with higher efficacy and lower toxicity from natural products, this review summarizes the more recent information on trypanocidal activities of various sesquiterpenes

  2. Intramolecular Morita-Baylis-Hillman reaction as a strategy for the construction of tricyclic sesquiterpene cores.

    Science.gov (United States)

    Peter, Clovis; Geoffroy, Philippe; Miesch, Michel

    2018-02-21

    Starting from a common polyfunctionalized bicyclo[3.2.1]octane-6,8-dione intermediate, a concise synthetic route to tricyclic cores found in quadrane, suberosane, cedrane and related sesquiterpenes was developed using a Morita-Baylis-Hillman intramolecular reaction as a key step.

  3. Diurnal and seasonal variation of monoterpene and sesquiterpene emissions from Scots pine ( Pinus sylvestris L.)

    Science.gov (United States)

    Holzke, C.; Hoffmann, T.; Jaeger, L.; Koppmann, R.; Zimmer, W.

    Recent research pointed out the question of missing OH reactivity in a forest system and the question for unknown highly reactive biogenic emissions. In this study we show that coniferous forests are an important source of highly reactive hydrocarbons, the sesquiterpenes. We investigated the seasonality of terpene emissions from Scots pine to work out influences on atmospheric chemistry in different seasons for both mono- and sesquiterpenes. Especially sesquiterpenes (C 15) change dramatically in their contribution to the terpene emissions of Scots pine. Fourteen sesquiterpenes and oxygenated compounds were found in the emissions. In spring, the pattern was most complex with all 14 compounds being emitted, whereas in summer and fall it was reduced to 1,8-cineol and camphor. The emission pattern of the monoterpenes varied only slightly. The main compounds emitted were α-pinene, β-pinene, and 3-carene representing up to 90% of the total terpene emission. The total monoterpene emission rates varied from below detection limit to 460 pmol m -2 s -1 with highest emission rates found in June. Standard emission rates of the main compounds calculated from the monthly measured diurnal emission courses varied considerably over the year. Highest values were found in spring and early summer with up to 700 pmol m -2 s -1.

  4. Anti-allergic activity of sesquiterpenes from the rhizomes of Cyperus rotundus.

    Science.gov (United States)

    Jin, Jeong Ho; Lee, Dong-Ung; Kim, Yeong Shik; Kim, Hyun Pyo

    2011-02-01

    From the 70% ethanol extract of the rhizomes of Cyperus rotundus (CRE), several major constituents including the sesquiterpene derivatives (valencene, nootkatone, and caryophyllene α-oxide), monoterpenes (β-pinene, 1,8-cineole, and limonene) and 4-cymene were isolated and examined for their anti-allergic activity in vitro and in vivo. In rat basophilic leukemia (RBL)-1 cells, the sesquiterpenes strongly inhibited 5-lipoxygenase-catalyzed leukotrienes production. In addition, they inhibited β-hexosaminidase release by antigen-stimulated RBL-2H3 cells, with valencene having the highest inhibitory effect. CRE inhibited leukotrienes production and β-hexosaminidase release at 300 μg/mL. It was also found that the most active sesquiterpene (valencene) and CRE inhibited β-hexosaminidase degranulation by inhibiting the initial activation reaction, Lyn phosphorylation, in IgE-stimulated RBL-2H3 cells. Moreover, CRE, valencene and nootkatone significantly inhibited the delayed-type hypersensitivity reaction in mice when administered orally at 50-300 mg/kg. In conclusion, C. rotundus and its constituents, valencene, nootkatone, and caryophyllene α-oxide, exert anti-allergic activity in vitro and in vivo. These sesquiterpenes, but not monoterpenes, certainly contribute to the anti-allergic activity of the rhizomes of C. rotundus.

  5. Hydrioxylation of sesquiterpenes by enzymes from chicory (Cichorium intybus L.) roots

    NARCIS (Netherlands)

    Kraker, de J.W.; Schurink, M.; Franssen, M.C.R.; König, W.A.; Groot, de Æ.; Bouwmeester, H.J.

    2003-01-01

    A microsomal enzyme preparation of chicory roots catalyses the hydroxylation of various sesquiterpene olefins in the presence of NADPH. Most of these hydroxylations take place at an isopropenyl or isopropylidene group. The number of products obtained from any of the substrates is confined to one or,

  6. Sesquiterpene lactones. XXXV. Constituents of Reichardia tingitana L. Roth. and their antifeedant activity

    Directory of Open Access Journals (Sweden)

    Włodzimierz M. Daniewski

    2014-01-01

    Full Text Available Reinvestigation of the components of Reichardia tingitana L. Roth. afforded besides already known sesquiterpenic lactones, 14-deoxylactucin (II and desacetylmatricarin (III cholesterol (V, stigmasterol (VI, β-sitosterol (VII and ceryl alcohol (VIII. The insect deterrent activity of compounds II and III has been determined.

  7. Structure, cytotoxic activity and mechanism of protoilludane sesquiterpene aryl esters from the mycelium of Armillaria mellea.

    Science.gov (United States)

    Li, Zhijin; Wang, Yunchao; Jiang, Bin; Li, Wenliang; Zheng, Lihua; Yang, Xiaoguang; Bao, Yongli; Sun, Luguo; Huang, Yanxin; Li, Yuxin

    2016-05-26

    Armillaria mellea (Vahl. ex. Fr.) Karst is an important traditional Chinese medicine used in dispelling wind and removing obstruction in the meridians, and strengthening tendons and bones. Armillaria mellea has been recorded in the book Caobenshiyi which was written by ancestor for the function of suppressing hyderactive liver for calming endogenous wind medicine. The aim of this study is to investigate the cytotoxic activity for liver cell lines (normal and cancerous) of protoilludane sesquiterpene aryl esters from the mycelium of A. mellea. A systemic fractionation of the mycelium extracts of A. mellea and relative activity mechanisms were studied. Two new protoilludane sesquiterpene aryl esters named 5'-methoxy-armillasin (1) and 5-hydroxyl-armillarivin (2) were isolated. In addition, eight known protoilludane sesquiterpene aryl esters armillaridin (3), armillartin (4), armillarin (5), melleolide B (6), armillarilin (7), armillasin (8), armillarigin (9) and melleolide (10) were also isolated from the mycelium of A. mellea. The relative configurations of the two new compounds were confirmed by NOESY spectra. Among ten protoilludane sesquiterpene aryl esters, compounds 2, 3, 4, 7, 8, 9 and 10 were active constituents with highly cytotoxic activity against HepG2 cells (4.95-37.65μg/mL). We reported here for the time, that compound 10 (melleolide) showed anti-tumor ability on hepatoma cell. The relative mechanism was assessed on HepG2 cells. Among all the ten protoilludane sesquiterpene aryl esters, melleolide (10) showed the best cytotoxic activity for HepG2 cells (4.95μg/mL) and lower activity for L02 cells (16.05μg/mL). Mechanism study showed that melleolide decreased the viability of the cancer cells with varying levels of cleaved-caspase 3, caspase 8, caspase 9, Bax and Ki67 expression. On the other hand, melleolide induced HepG2 cell cycle arrest at the G2/M phase. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  8. Mode of Action of the Sesquiterpene Lactones Psilostachyin and Psilostachyin C on Trypanosoma cruzi.

    Directory of Open Access Journals (Sweden)

    Valeria P Sülsen

    Full Text Available Trypanosoma cruzi is the causative agent of Chagas' disease, which is a major endemic disease in Latin America and is recognized by the WHO as one of the 17 neglected tropical diseases in the world. Psilostachyin and psilostachyin C, two sesquiterpene lactones isolated from Ambrosia spp., have been demonstrated to have trypanocidal activity. Considering both the potential therapeutic targets present in the parasite, and the several mechanisms of action proposed for sesquiterpene lactones, the aim of this work was to characterize the mode of action of psilostachyin and psilostachyin C on Trypanosoma cruzi and to identify the possible targets for these molecules. Psilostachyin and psilostachyin C were isolated from Ambrosia tenuifolia and Ambrosia scabra, respectively. Interaction of sesquiterpene lactones with hemin, the induction of oxidative stress, the inhibition of cruzipain and trypanothione reductase and their ability to inhibit sterol biosynthesis were evaluated. The induction of cell death by apoptosis was also evaluated by analyzing phosphatidylserine exposure detected using annexin-V/propidium iodide, decreased mitochondrial membrane potential, assessed with Rhodamine 123 and nuclear DNA fragmentation evaluated by the TUNEL assay. Both STLs were capable of interacting with hemin. Psilostachyin increased about 5 times the generation of reactive oxygen species in Trypanosoma cruzi after a 4h treatment, unlike psilostachyin C which induced an increase in reactive oxygen species levels of only 1.5 times. Only psilostachyin C was able to inhibit the biosynthesis of ergosterol, causing an accumulation of squalene. Both sesquiterpene lactones induced parasite death by apoptosis. Upon evaluating the combination of both compounds, and additive trypanocidal effect was observed. Despite their structural similarity, both sesquiterpene lactones exerted their anti-T. cruzi activity through interaction with different targets. Psilostachyin

  9. Mode of Action of the Sesquiterpene Lactones Psilostachyin and Psilostachyin C on Trypanosoma cruzi.

    Science.gov (United States)

    Sülsen, Valeria P; Puente, Vanesa; Papademetrio, Daniela; Batlle, Alcira; Martino, Virginia S; Frank, Fernanda M; Lombardo, María E

    2016-01-01

    Trypanosoma cruzi is the causative agent of Chagas' disease, which is a major endemic disease in Latin America and is recognized by the WHO as one of the 17 neglected tropical diseases in the world. Psilostachyin and psilostachyin C, two sesquiterpene lactones isolated from Ambrosia spp., have been demonstrated to have trypanocidal activity. Considering both the potential therapeutic targets present in the parasite, and the several mechanisms of action proposed for sesquiterpene lactones, the aim of this work was to characterize the mode of action of psilostachyin and psilostachyin C on Trypanosoma cruzi and to identify the possible targets for these molecules. Psilostachyin and psilostachyin C were isolated from Ambrosia tenuifolia and Ambrosia scabra, respectively. Interaction of sesquiterpene lactones with hemin, the induction of oxidative stress, the inhibition of cruzipain and trypanothione reductase and their ability to inhibit sterol biosynthesis were evaluated. The induction of cell death by apoptosis was also evaluated by analyzing phosphatidylserine exposure detected using annexin-V/propidium iodide, decreased mitochondrial membrane potential, assessed with Rhodamine 123 and nuclear DNA fragmentation evaluated by the TUNEL assay. Both STLs were capable of interacting with hemin. Psilostachyin increased about 5 times the generation of reactive oxygen species in Trypanosoma cruzi after a 4h treatment, unlike psilostachyin C which induced an increase in reactive oxygen species levels of only 1.5 times. Only psilostachyin C was able to inhibit the biosynthesis of ergosterol, causing an accumulation of squalene. Both sesquiterpene lactones induced parasite death by apoptosis. Upon evaluating the combination of both compounds, and additive trypanocidal effect was observed. Despite their structural similarity, both sesquiterpene lactones exerted their anti-T. cruzi activity through interaction with different targets. Psilostachyin accomplished its antiparasitic

  10. 9-Hydroxyfurodysinin-O-ethyl Lactone: A New Sesquiterpene Isolated from the Tropical Marine Sponge Dysidea arenaria

    Directory of Open Access Journals (Sweden)

    P. Karuso

    2005-10-01

    Full Text Available A new sesquiterpene, 9-hydroxyfurodysinin-O-ethyl lactone, has been isolated from a New Caledonian Dysidea arenaria, along with three known compounds. The possible incorporation of the ethyl ether from the extraction solvent is discussed.

  11. New Sesquiterpene Oxidations with CYP260A1 and CYP264B1 from Sorangium cellulosum So ce56.

    Science.gov (United States)

    Schifrin, Alexander; Litzenburger, Martin; Ringle, Michael; Ly, Thuy T B; Bernhardt, Rita

    2015-12-01

    Sesquiterpenes are natural products derived from the common precursor farnesyl pyrophosphate (FPP) but are highly diverse in structure and function. Cytochrome P450 enzymes (P450s) exhibit the unique ability to introduce molecular oxygen into non-activated C-H bonds. In plant biosynthetic pathways, P450s commonly derivatize sesquiterpene hydrocarbons. However, the potential of bacterial P450s for terpene derivatization is still underinvestigated. This work compares the substrate specificities and regioselectivities of the sesquiterpene hydroxylases CYP260A1 and CYP264B1 from myxobacterium Sorangium cellulosum So ce56. Four tested substrate classes (eremophilanes, humulanes, caryophyllanes, and cedranes) were converted by both P450s. The achievable variety of oxidations is demonstrated on the model substrates (+)-nootkatone and zerumbone. Increasing the number of functionally investigated P450s, this study represents a step towards the selective derivatization of sesquiterpenes. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. Sesquiterpenes from Ulmus davidiana var. japonica with the inhibitory effects on lipopolysaccharide-induced nitric oxide production.

    Science.gov (United States)

    Kim, Young Choong; Lee, Mi Kyeong; Sung, Sang Hyun; Kim, Seung Hyun

    2007-04-01

    Investigation of antiinflammatory constituents of the stem and root barks of Ulmus davidiana var. japonica resulted in the isolation of three guaiane type sesquiterpenes, torilin, 1-hydroxytorilin, together with a new derivative, (1beta, 7beta, 8beta, 10beta)-1,8,11-trihydroxy-4-guaien-8-angeloyl-3-one named 1-hydroxytorilin A. All the three sesquiterpenes inhibited lipopolysaccharide-induced nitric oxide production in murine microglial BV2 cells.

  13. Studies on the Expression of Sesquiterpene Synthases Using Promoter-?-Glucuronidase Fusions in Transgenic Artemisia annua L

    OpenAIRE

    Wang, Hongzhen; Han, Junli; Kanagarajan, Selvaraju; Lundgren, Anneli; Brodelius, Peter E.

    2013-01-01

    In order to better understand the influence of sesquiterpene synthases on artemisinin yield in Artemisia annua, the expression of some sesquiterpene synthases has been studied using transgenic plants expressing promoter-GUS fusions. The cloned promoter sequences were 923, 1182 and 1510 bp for β-caryophyllene (CPS), epi-cedrol (ECS) and β-farnesene (FS) synthase, respectively. Prediction of cis-acting regulatory elements showed that the promoters are involved in complex regulation of expressio...

  14. Sesquiterpenes and other constituents from leaves of Pterodon pubescens Benth (Leguminosae)

    International Nuclear Information System (INIS)

    Miranda, Mayker Lazaro Dantas; Garcez, Fernanda Rodrigues; Garcez, Walmir Silva

    2014-01-01

    In addition to β-sitosterol, stigmasterol, phaeophitin A, luteolin, kaempferol, quercetin, (+)-catechin, quercetin-3-O-α-L-rhamnopyranoside, rutin, and p-hydroxy-benzoic acid, six known sesquiterpenes, namely (rel)-2β,6β-epoxy-5β-hydroxy-isodaucane, oplopanone, 1β,6α-dihydroxy-4(15)-eudesmene, caryophyllene oxide, α-cadinol, and spathulenol, were isolated from the leaves of Pterodon pubescens (Leguminosae) growing in the Cerrado of Mato Grosso do Sul, Brazil. The (rel)-2β,6β-epoxy-5β-hydroxy-isodaucane corresponds to the correct structure of homalomenol D. The sesquiterpene oplopanone, which bears a modified cadinane skeleton, is being reported for the first time in this genus. The structures of the compounds were determined on the basis of spectral data (MS, IR, and NMR-1D and 2D) and subsequent comparison with data reported in the literature. (author)

  15. Biosynthesis of the sesquiterpene germacrene D in Solidago canadensis: 13C and (2)H labeling studies.

    Science.gov (United States)

    Steliopoulos, Panagiotis; Wüst, Matthias; Adam, Klaus-Peter; Mosandl, Armin

    2002-05-01

    The biogenetic origin of the isoprenoid building blocks of the sesquiterpene germacrene D was studied in Solidago canadensis. Feeding experiments were carried out with 1-[5,5-D(2)]deoxy-D-xylulose-5-phosphate (D(2)-DOXP), [5-13C]mevalonolactone (13C-MVL) and [1-13C]-D-glucose. The hydrodistillate of a cut shoot fed with D(2)-DOXP was investigated by enantio-MDGC-MS and the volatile fraction of a shoot supplied with 13C-MVL was examined by GC-C-IRMS. The incorporation of [1-13C]-D-glucose was analyzed by quantitative 13C NMR spectroscopy after isolation of germacrene D from the essential oil. Our labeling studies revealed that the biosynthesis of the C-15 skeleton of sesquiterpene germacrene D in Solidago canadensis proceeds predominantly via the methylerythritol phosphate pathway.

  16. Antimicrobial drimane sesquiterpenes and their effect on endophyte communities in the medical tree Warburgia ugandensis

    Directory of Open Access Journals (Sweden)

    Sigrid eDrage

    2014-02-01

    Full Text Available Metabolite profiles (GC–MS, drimane sesquiterpenes, sugars and sugar alcohols, were compared with bacterial and fungal endophyte communities (T-RFLP, DNA clones, qPCR in leaves and roots of the pepper bark tree, Warburgia ugandensis (Canellaceae. Ten individuals each were assessed from two locations east and west of the Great Rift Valley, Kenya, Africa, which differed in humidity and vegetation, closed forest versus open savannah. Despite organ- and partially site-specific variation of drimane sesquiterpenes, no clear effects on bacterial and fungal endophyte communities could be detected. The former were dominated by gram-negative Gammaproteobacteria, Pseudomonadaceae and Enterobacteriaceae, as well as gram-positive Firmicutes; the fungal endophyte communities were more diverse but no specific groups dominated. Despite initial expectations, the endophyte community of the pepper bark tree did not differ from other trees that much.

  17. Two New Eremophilane Sesquiterpenes and One New Resorcinol from Ligularia knorringiana.

    Science.gov (United States)

    Dai, Wei; Li, Hui; Cong, Wenli; Zhao, Wenbin; Wang, Qi

    2016-02-01

    Two new eremophilane sesquiterpenes, 12-oxa-1β, 5β,8,9aβ-tetramethyl-4α,5-ethano-4aβ-hydroxy-7β,10aβ-epoxy-9β-angeloyloxy-1,2,3,4,4a,5,6,7,9,9a,10,10a- dodecahydro-anthracen-13-on (knorringianalarin A, 1) and 6,9-dien-8-oxoeremophil-12-nor-11-ketone (knorringianalarin B, 2), and a new resorcinol, 4-acetyl- 6-(2-methylpropionyl)-1,3-resorcinol (knorringianalarin C, 3) were isolated from the roots and rhizomes of Ligularia knorringiana, together with three known eremophilane sesquiterpenes (4-6). The structures of the new compounds were identified by spectroscopic methods including 2D-NMR techniques.

  18. Cytotoxic and Pro-apoptotic Activities of Sesquiterpene Lactones from Inula britannica.

    Science.gov (United States)

    Xiang, Ping; Guo, Xin; Han, Yang-Yang; Gao, Jin-Ming; Tang, Jiang-Jiang

    2016-01-01

    In this study, five known sesquiterpene lactones (STL) with an α-methylene-γ-lactone motif, including two eudesmanolides, 1β-hydroxyalantolactone (1) and ivangustin (2), and three 1,10-seco-eudesmanolides, 1-O-acetylbritannilactone (3), 1,6-O,O-diacetylbritannilactone (4), and 6α-O-(2- methylbutyryl)britannilactone (5) were isolated from the flower heads of the medicinal plant Inula britannica. Their structures were characterized by spectroscopic methods. X-ray data of 2 is reported for the first time. Among them, eudesmanolides 1 and 2 exhibited remarkable cytotoxicity against HEp2, SGC-7901 and HCT116 human cancer cell lines, comparable with etoposide (Vp-16) used as reference drug. Furthermore, treatment of HEp2 cells with 1 induced apoptosis associated with cleaved procaspase-3 and PARP. The biological assays carried out with normal cells (CHO) revealed that all sesquiterpenes were weakly selective against the cancer cell lines tested.

  19. Four new sesquiterpenes from the rhizomes of Curcuma phaeocaulis and their iNOS inhibitory activities.

    Science.gov (United States)

    Ma, Jiang-Hao; Wang, Ying; Liu, Yue; Gao, Su-Yu; Ding, Li-Qin; Zhao, Feng; Chen, Li-Xia; Qiu, Feng

    2015-05-01

    Three new guaiane-type sesquiterpenes named phaeocaulisins K-M (1-3), and one germacrane-type sesquiterpenoid with new ring system of 1,5- and 1,8-ether groups named phagermadiol (4), were isolated from rhizomes of Curcuma phaeocaulis. Their structures were established based on extensive spectroscopic analysis. Compound 1, the first example of norsesquiterpene with tropone backbone, and compound 3 with a novel 1,2-dioxolane sesquiterpene alcohol were isolated from the genus Curcuma. All of the isolated compounds were tested for inhibitory activity against lipopolysaccharide-induced nitric oxide (NO) production in RAW 264.7 macrophages. Compound 3 inhibited NO production with IC50 value of 6.05 ± 0.43 μM. The plausible biosynthetic pathway for compounds 3 and 4 in C. phaeocaulis was also discussed.

  20. (+)-4 beta-hydroxyhernandulcin, a new sweet sesquiterpene from the leaves and flowers of Lippia dulcis.

    Science.gov (United States)

    Kaneda, N; Lee, I S; Gupta, M P; Soejarto, D D; Kinghorn, A D

    1992-08-01

    From the leaves and flowers of Lippia dulcis collected in Panama, a new sweet sesquiterpene identified as (+)-4 beta-hydroxyhernandulcin [2] was isolated, accompanied by (+)-hernandulcin [1], (-)-epihernandulcin [3] (a novel natural product), and 6-methyl-5-hepten-2-one [4]. Acteoside (verbascoside) [5], a known bitter phenylpropanoid glycoside, was isolated from the flowers of L. dulcis. The structure of (+)-4 beta-hydroxyhernandulcin was established by interpretation of its spectral data.

  1. Targeted modification of trilobolide and search for related sesquiterpenes with immunobiological properties

    Czech Academy of Sciences Publication Activity Database

    Harmatha, Juraj; Buděšínský, Miloš; Vokáč, Karel; Kmoníčková, Eva; Zídek, Zdeněk

    2011-01-01

    Roč. 77, č. 12 (2011), s. 1400-1400 ISSN 0032-0943. [International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research /59./. 04.09.2011-09.09.2011, Antalya] R&D Projects: GA ČR GA305/07/0061 Institutional research plan: CEZ:AV0Z40550506; CEZ:AV0Z50390512 Keywords : sesquiterpenes * guaianolides * trilobolide * immunomodulation Subject RIV: CC - Organic Chemistry

  2. Sesquiterpene lactones. XXXIII. Guaianolides in the subgenus Psephellus (Cass. Schmalh., genus Centaurea L.

    Directory of Open Access Journals (Sweden)

    Gerard Nowak

    2014-01-01

    Full Text Available Sesquiterpene lactones were found to occur in all of the studied species of the subgenus Psephellus (Cass. Schmalh. Differing compositions were found in the representatives of three sections. In Centaurea declinata MB. from the section Leucophylle (Sosn. Sosn.. 15-deoxyrepin, linichlorin B and cynaropicrin were found. Linichlorin B dominated in Centaurea hypoleucu DC. from section Hypoleucae (Sosn. Sosn., while in the species classified in section Psephellus Sosn., repin, acroptilin, jenerin, centaurepensin and, in some, also cynaropicrin. dominated.

  3. Dihydro-β-agarofuran sesquiterpene pyridine alkaloids from the seeds of Euonymus hamiltonianus

    Directory of Open Access Journals (Sweden)

    Mudasir A. Tantry

    2016-09-01

    Full Text Available Plants of the Celastraceae family produce various dihydro-β-agarofuran sesquiterpene pyridine alkaloids. Two dihydro-β-agarofuran sesquitepene pyridine alkaloids (1,2 apart from four known compounds euojaponin C (3, wilforine (4, austronine (5 and O9-benzoyl-O9-deacetylevonine (6, were isolated from the ripe seeds of Euonymus hamiltonianus. Their chemical structures were elucidated mainly by analysis of NMR and MS spectral data. All compounds were evaluated for insecticidal activity.

  4. Hepatocurative potential of sesquiterpene lactones of Taraxacum officinale on carbon tetrachloride induced liver toxicity in mice.

    Science.gov (United States)

    Mahesh, A; Jeyachandran, R; Cindrella, L; Thangadurai, D; Veerapur, V P; Muralidhara Rao, D

    2010-06-01

    The hepatocurative potential of ethanolic extract (ETO) and sesquiterpene lactones enriched fraction (SL) of Taraxacum officinale roots was evaluated against carbon tetrachloride (CCl 4 ) induced hepatotoxicity in mice. The diagnostic markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin contents were significantly elevated, whereas significant reduction in the level of reduced glutathione (GSH) and enhanced hepatic lipid peroxidation, liver weight and liver protein were observed in CCl 4 induced hepatotoxicity in mice. Post-treatment with ETO and SL significantly protected the hepatotoxicity as evident from the lower levels of hepatic enzyme markers, such as serum transaminase (ALT, AST), ALP and total bilirubin. Further, significant reduction in the liver weight and liver protein in drug-treated hepatotoxic mice and also reduced oxidative stress by increasing reduced glutathione content and decreasing lipid peroxidation level has been noticed. The histopathological evaluation of the liver also revealed that ETO and SL reduced the incidence of liver lesions induced by CCl 4 . The results indicate that sesquiterpene lactones have a protective effect against acute hepatotoxicity induced by the administration of CCl 4 in mice. Furthermore, observed activity of SL may be due to the synergistic action of two sesquiterpene lactones identified from enriched ethyl acetate fraction by HPLC method.

  5. Developmentally regulated sesquiterpene production confers resistance to Colletotrichum gloeosporioides in ripe pepper fruits.

    Directory of Open Access Journals (Sweden)

    Sangkyu Park

    Full Text Available Sesquiterpenoid capsidiol, exhibiting antifungal activity against pathogenic fungus, is accumulated in infected ripe pepper fruits. In this study, we found a negative relation between the capsidiol level and lesion size in fruits infected with Colletotrichum gloeosporioides, depending on the stage of ripening. To understand the developmental regulation of capsidiol biosynthesis, fungal-induced gene expressions in the isoprenoid biosynthetic pathways were examined in unripe and ripe pepper fruits. The sterol biosynthetic pathway was almost shut down in healthy ripe fruits, showing very low expression of hydroxymethyl glutaryl CoA reductase (HMGR and squalene synthase (SS genes. In contrast, genes in the carotenoid pathway were highly expressed in ripe fruits. In the sesquiterpene pathway, 5-epi-aristolochene synthase (EAS, belonging to a sesquiterpene cyclase (STC family, was significantly induced in the ripe fruits upon fungal infection. Immunoblot and enzyme activity analyses showed that the STCs were induced both in the infected unripe and ripe fruits, while capsidiol was synthesized discriminatively in the ripe fruits, implying diverse enzymatic specificity of multiple STCs. Thereby, to divert sterol biosynthesis into sesquiterpene production, infected fruits were pretreated with an SS inhibitor, zaragozic acid (ZA, resulting in increased levels of capsidiol by more than 2-fold in the ripe fruits, with concurrent reduction of phytosterols. Taken together, the present results suggest that the enhanced expression and activity of EAS in the ripe fruits play an important role in capsidiol production, contributing to the incompatibility between the anthracnose fungus and the ripe pepper fruits.

  6. Sesquiterpenes from fruits of Torilis japonica with inhibitory activity on melanin synthesis in B16 cells.

    Science.gov (United States)

    Song, Da Hye; Jo, Yang Hee; Ahn, Jong Hoon; Kim, Seon Beom; Yun, Cheong-Yong; Kim, Youngsoo; Hwang, Bang Yeon; Lee, Mi Kyeong

    2018-01-01

    Melanin, a dark macromolecular pigment, protects skin from harmful damage. However, abnormal accumulation is responsible for hyperpigmentation disorders. Melanogenesis inhibitors have therefore become important constituents in cosmetic products for depigmentation. Torilis japonica Decandolle (Umbelliferae) is a biennial plant which is distributed in East Asia. Fruits of this plant have been used for the treatment of skin disease and inflammation. In our previous study, torilin, a major sesquiterpene of T. japonica, showed an inhibitory effect on melanin production in α-melanocyte stimulating hormone (α-MSH)-activated B16 melanoma cells. Further extensive chromatographic separation resulted in thirteen compounds. On the basis of spectroscopic analysis, the structures of the compounds isolated were determined to be three new sesquiterpenes, viz. a guaiane-type, epoxytorilinol (1), a eudesmane-type, elematorilone (2) and a cadinane-type, cardinatoriloside (3), together with ten known sesquiterpenes (4-13). Of the compounds isolated, compounds 4-6 and 11-13 inhibited α-MSH-activated melanin production in B16 melanoma cells with IC 50 values from 72.9 to 191.0 μM.

  7. Supercritical fluid extraction assisted isolation of sesquiterpene lactones with antiproliferative effects from Centipeda minima.

    Science.gov (United States)

    Wu, Peng; Su, Miao-Xian; Wang, Ying; Wang, Guo-Cai; Ye, Wen-Cai; Chung, Hau-Yin; Li, Juan; Jiang, Ren-Wang; Li, Yao-Lan

    2012-04-01

    Pseudoguaianolide sesquiterpene lactones minimolides A (1), B (2), C (3) and D (4) and two guaianolide sesquiterpene lactones minimolides E (5) and F (6), along with seven known ones (7-13), were isolated from the supercritical fluid extract of Centipeda minima. The structures of these compounds were elucidated by extensive spectroscopic methods (IR, UV, HRESIMS, 1D-NMR and 2D-NMR), and the complete structure and stereochemistry of 1 was further confirmed by X-ray diffraction analysis. Compounds 1, 5-8,11 and 13 displayed inhibitory activity against human nasopharyngeal cancer cells (CNE) with IC(50) values ranging from 1.1 to 20.3 μM. Compound 13 containing both α-methylene-γ-lactone and α, β-unsaturated cyclopentenone moieties exhibited even stronger inhibitory activity than that of cisplatin (positive control) through cell cycle arrest at G2/M phase. Isolation of six sesquiterpene lactones from Centipeda minima highlighted the potential of supercritical fluid extraction for enrichment of minor constituents for phytochemical study. Copyright © 2012 Elsevier Ltd. All rights reserved.

  8. Sesquiterpenes evaluation on Schistosoma mansoni: Survival, excretory system and membrane integrity.

    Science.gov (United States)

    de Oliveira, Rosimeire Nunes; Dos Santos, Karina Rodrigues; Mendes, Tiago Manuel Fernandes; Garcia, Vera Lúcia; Santos Oliveira, Adriana Silva; de Lourdes Sierpe Jeraldo, Veronica; Allegretti, Silmara Marques

    2017-06-01

    The main challenge in schistosomiasis control has been the emergence of drug-resistant parasites. Since the 1970's, praziquantel (PZQ) is the single drug for treatment. This fact highlights the importance to research news chemotherapeutic agents. In the last years, S. mansoni excretory system and tegument have been major targets for drug development. The purpose of this study was to evaluate the effect of sesquiterpenes, alpha-humulene and trans-caryophyllene on S. mansoni survival, excretory system and membrane integrity, after in vitro exposure. The in vitro studies, showed that sesquiterpenes reduced egg production and motor activity of worms at sublethal concentrations, and caused death in a concentration-dependent manner (100 and 200μg/mL). Tegumental analysis by Scanning Electron Microscopy (SEM), showed tegument damage. Additionally, it was possible to observe lesions, evidenced by intense marking trough Hoechst probe, in the tegument and suckers of worms exposed to 200μg/mL. In this study, we also showed that resorufin is only capable of identifying the interaction of sesquiterpenes in males excretory system, Pgp expression and inferring that females are more tolerant to treatments. Thus, the present study results contribute to an understanding of alpha-humulene and trans-caryophyllene effect over these targets, contributing for the development of schistosomicidal drugs. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  9. SESQUITERPENE RICH VOLATILE SEED OIL OF TAGETES PATULA L. FROM NORTHWEST IRAN

    Directory of Open Access Journals (Sweden)

    M. B. HASSANPOURAGHDAM

    2011-10-01

    Full Text Available Hydrodistilled volatile seed oil composition of commonly growing ornamental Tagetes patula L. was analyzed for its constituents by GC/MS. Forty constituents were identified, comprising 94% of the total oil. Sesquiterpene hydrocarbons (52.7% and oxygenated sesquiterpenes (15.8% were the main subclasses of volatile oil components followed by monoterpene hydrocarbons (12.6%. The principle constituents of the volatile oil were (E-caryophyllene (44.6% caryophyllene oxide (14.8%, germacrene D (3.8%, (Z-β-ocimene (3.8% and limonene (3.7%. From chemical point of view, oxides (15.7% were the predominant group of components with caryophyllene oxide as their main representative. α-terthienyl (3.8% comprised partially large amount in the volatile oil content despite of its polar and less-volatile nature. Taking into account the volatile oil profile, the chemical composition of the volatile seed oil of commonly growing ornamental T. patula L. was characterized as sesquiterpene and α-terthienyl rich one probably with appreciable biocidal (Insecticidal and nematicidal and pharmacological potential.

  10. A modeling study of secondary organic aerosol formation from sesquiterpenes using the STOCHEM global chemistry and transport model

    Science.gov (United States)

    Khan, M. A. H.; Jenkin, M. E.; Foulds, A.; Derwent, R. G.; Percival, C. J.; Shallcross, D. E.

    2017-04-01

    Sesquiterpenes are one of the precursors of secondary organic aerosol (SOA) which can be an important global sources of organic aerosol (OA). Updating the chemistry scheme in the global chemistry transport model by incorporating an oxidation mechanism for β-caryophyllene (representing all sesquiterpenes), adding global sesquiterpene emissions of 29 Tg/yr, and revising global monoterpene emissions up to 162 Tg/yr [Guenther et al., 2012] led to an increase of SOA burden by 95% and SOA production rate by 106% relative to the base case described in Utembe et al. [2011]. Including the emissions of sesquiterpenes resulted in increase of SOA burden of 0.11 Tg and SOA production rate of 12.9 Tg/yr relative to the base case. The highest concentrations of sesquiterpene-derived SOA (by up to 1.2 μg/m3) were found over central Africa and South America, the regions having high levels of biogenic emissions with significant biomass burning. In the updated model simulation, the multigeneration oxidation products from sesquiterpenes and monoterpenes transported above the boundary layer and condensed to the aerosol phase at higher altitude led to an increase of OA by up to 30% over the tropics and northern midlatitude to higher altitude. The model evaluation showed an underestimation of model OA mostly for the campaigns dominated by regional anthropogenic pollution. The increase of SOA production from sesquiterpenes reduced the discrepancies between modeled and observed OA concentrations over the remote and rural areas. The increase of SOA concentrations by up to 200% from preindustrial to present scenarios was found over the tropical oceans.

  11. Simultaneous determination of multiple sesquiterpenes in Curcuma wenyujin herbal medicines and related products with one single reference standard.

    Science.gov (United States)

    Zhu, Jing-Jing; An, Yue-Wei; Hu, Guang; Yin, Guo-Ping; Zhang, Qi-Wei; Wang, Zhi-Min

    2013-02-06

    Some Curcuma species are widely used as herbal medicines. Sesquiterpenes are their important bioactive compounds and their quantitative analysis is generally accomplished by gas chromatography (GC) or high performance liquid chromatography (HPLC), but the instability and high cost of some sesquiterpene reference standards have limited their application. It is necessary to find a practicable means to control the quality of herbal medicines. Using one stable component contained in Curcuma species to determine multiple analogues should be a practical option. In this study, a simple HPLC method for determination of sesquiterpenes using relative response factors (RRFs) has been developed. The easily available and stable active component curdione was selected as the reference compound for calculating the RRFs of the other eight sesquiterpenes, including zedoarondiol (Zedo), isozedoarondiol (Isoz), aerugidiol (Aeru), (4S,5S)-(+)-germacrone-4,5-epoxide (Epox), curcumenone (Curc), neocurdione (Neoc), germacrone (Germ) and furanodiene (Fura). Their RRFs against curdione were between 0.131-1.301, with a good reproducibility. By using the RRFs, the quantification of sesquiterpenes in Curcuma wenyujin herbal medicines and related products was carried out. The method is especially useful for the determination of (4S,5S)-(+)-germacrone-4,5-epoxide, curcumenone, germacrone and furanodiene, which often are regarded as the principle components in Curcuma species, but unstable when were purified. It is an ideal means to analyze the components for which reference standards are not readily available.

  12. Antifungal activities of the essential oil and its fractions rich in sesquiterpenes from leaves of Casearia sylvestris Sw.

    Science.gov (United States)

    Pereira, Flaviane G; Marquete, Ronaldo; Domingos, Levy T; Rocha, Marco E N; Ferreira-Pereira, Antonio; Mansur, Elisabeth; Moreira, Davyson L

    2017-01-01

    Casearia genus (Salicaceae) is found in sub-tropical and tropical regions of the world and comprises about 160-200 species. It is a medicinal plant used in South America, also known as "guaçatonga", "erva-de-tiú", "cafezinho-do-mato". In Brazil, there are about 48 species and 12 are registered in the State of Rio de Janeiro, including Casearia sylvestris Sw. There are many studies related to the chemical profile and cytotoxic activities of extracts from these plants, although few studies about the antifungal potential of the essential oil have been reported. In this work, we have studied the antifungal properties of the essential oil of C. sylvestris leaves, as well as of their fractions, against four yeasts (Saccharomyces cerevisae, Candida albicans, C. glabrata and C. krusei) for the first time. The chemical analysis of the essential oil revealed a very diversified (n = 21 compounds) volatile fraction composed mainly of non-oxygenated sesquiterpenes (72.1%). These sesquiterpenes included α-humulene (17.8%) and α-copaene (8.5%) and the oxygenated sesquiterpene spathulenol (11.8%) were also identified. Monoterpenes were not identified. The fractions are mainly composed of oxygenated sesquiterpenes, and the most active fraction is rich in the sesquiterpene 14-hydroxy -9-epi-β-caryophyllene. This fraction was the most effective in inhibiting the growth of three yeast strains.

  13. Antifungal activities of the essential oil and its fractions rich in sesquiterpenes from leaves of Casearia sylvestris Sw.

    Directory of Open Access Journals (Sweden)

    FLAVIANE G. PEREIRA

    2017-12-01

    Full Text Available ABSTRACT Casearia genus (Salicaceae is found in sub-tropical and tropical regions of the world and comprises about 160-200 species. It is a medicinal plant used in South America, also known as “guaçatonga”, “erva-de-tiú”, “cafezinho-do-mato”. In Brazil, there are about 48 species and 12 are registered in the State of Rio de Janeiro, including Casearia sylvestris Sw. There are many studies related to the chemical profile and cytotoxic activities of extracts from these plants, although few studies about the antifungal potential of the essential oil have been reported. In this work, we have studied the antifungal properties of the essential oil of C. sylvestris leaves, as well as of their fractions, against four yeasts (Saccharomyces cerevisae, Candida albicans, C. glabrata and C. krusei for the first time. The chemical analysis of the essential oil revealed a very diversified (n = 21 compounds volatile fraction composed mainly of non-oxygenated sesquiterpenes (72.1%. These sesquiterpenes included α-humulene (17.8% and α-copaene (8.5% and the oxygenated sesquiterpene spathulenol (11.8% were also identified. Monoterpenes were not identified. The fractions are mainly composed of oxygenated sesquiterpenes, and the most active fraction is rich in the sesquiterpene 14-hydroxy -9-epi-β-caryophyllene. This fraction was the most effective in inhibiting the growth of three yeast strains.

  14. Tandem Mass Spectrometry for Structural Identification of Sesquiterpene alkaloids from the stems of dendrobium nobile using LC-QToF3

    Science.gov (United States)

    Dendrobium nobile is one of the fundamental herbs in traditional Chinese medicine (TCM). Sesquiterpene alkaloids are the main active components in this plant. Due to weak ultraviolet absorption and low content in D. nobile, these sesquiterpene alkaloids have not been extensively studied using chroma...

  15. Quorum sensing inhibitory potential and molecular docking studies of sesquiterpene lactones from Vernonia blumeoides.

    Science.gov (United States)

    Aliyu, Abubakar Babando; Koorbanally, Neil Anthony; Moodley, Brenda; Singh, Parvesh; Chenia, Hafizah Yousuf

    2016-06-01

    The increasing incidence of multidrug-resistant Gram-negative bacterial pathogens has focused research on the suppression of bacterial virulence via quorum sensing inhibition strategies, rather than the conventional antimicrobial approach. The anti-virulence potential of eudesmanolide sesquiterpene lactones previously isolated from Vernonia blumeoides was assessed by inhibition of quorum sensing and in silico molecular docking. Inhibition of quorum sensing-controlled violacein production in Chromobacterium violaceum was quantified using violacein inhibition assays. Qualitative modulation of quorum sensing activity and signal synthesis was investigated using agar diffusion double ring assays and C. violaceum and Agrobacterium tumefaciens biosensor systems. Inhibition of violacein production was concentration-dependent, with ⩾90% inhibition being obtained with ⩾2.4 mg ml(-1) of crude extracts. Violacein inhibition was significant for the ethyl acetate extract with decreasing inhibition being observed with dichloromethane, hexane and methanol extracts. Violacein inhibition ⩾80% was obtained with 0.071 mg ml(-1) of blumeoidolide B in comparison with ⩾3.6 mg ml(-1) of blumeoidolide A. Agar diffusion double ring assays indicated that only the activity of the LuxI synthase homologue, CviI, was modulated by blumeoidolides A and B, and V. blumeoides crude extracts, suggesting that quorum sensing signal synthesis was down-regulated or competitively inhibited. Finally, molecular docking was conducted to explore the binding conformations of sesquiterpene lactones into the binding sites of quorum sensing regulator proteins, CviR and CviR'. The computed binding energy data suggested that the blumeoidolides have a tendency to inhibit both CviR and CviR' with varying binding affinities. Vernonia eudesmanolide sesquiterpene lactones have the potential to be novel therapeutic agents, which might be important in reducing virulence and pathogenicity of drug-resistant bacteria

  16. Cadinane sesquiterpenes from Curcuma phaeocaulis with their inhibitory activities on nitric oxide production in RAW 264.7 cells.

    Science.gov (United States)

    Ma, Jianghao; Wang, Ying; Liu, Yue; Gao, Suyu; Ding, Liqin; Zhao, Feng; Chen, Lixia; Qiu, Feng

    2015-06-01

    Four new cadinane-type sesquiterpenes named phacadinanes A-D (1-4) were isolated from the rhizomes of Curcuma phaeocaulis. Their structures were elucidated by 1D and 2D NMR, as well as accurate mass measurements. Compound 4 was the first example of a rare 4,5-seco-cadinane sesquiterpene isolated from the Zingiberaceae family. Furthermore, inhibitory effects of the isolated compounds on nitric oxide production in LPS-activated macrophages were evaluated. Compounds 1 and 2 showed strong inhibitory activities on NO production with IC50 values of 3.88±0.58 and 2.25±0.71 μM, respectively. A possible biogenetic pathway for 4,5-seco-cadinane sesquiterpene (4) was postulated. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Flavonoids and sesquiterpenes of Croton pedicellatus Kunth; Flavonoides e sesquiterpenos de Croton pedicellatus Kunth.

    Energy Technology Data Exchange (ETDEWEB)

    Lopes, Elton Luz; Andrade Neto, Manoel; Silveira, Edilberto Rocha; Pessoa, Otilia Deusdenia Loiola [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Dept. de Quimica Organica e Inorganica; Braz-Filho, Raimundo [Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacases, RJ (Brazil). Dept. de Quimica

    2012-07-01

    The chemical investigation of the ethanolic extract from leaves of Croton pedicellatus yielded the bis-nor-sesquiterpenes blumenol A and blumenol A glucoside, along with the flavonoids: tiliroside, 6{sup -}O-p-coumaroyl-{beta}-galactopyranosyl- kaempferol, 6{sup -}O-p-coumaroyl-{beta}-glucopyranosyl-3{sup -}methoxy- kaempferol, kaempferol, 3-glucopyranosyl-quercetin and alpinumisoflavone, as well as 4-hydroxy-3,5-dimethoxybenzoic acid. The identification of all isolated compounds was performed by spectrometric methods, including HR-ESI-MS, 1D and 2D NMR experiments, and by comparison with previously-described physical and spectral data. (author)

  18. Diversion of flux toward sesquiterpene production in Saccharomyces cerevisiae by fusion of host and heterologous enzymes

    DEFF Research Database (Denmark)

    Albertsen, Line; Chen, Yun; Bach, Lars Stougaard

    2011-01-01

    in the pathway are expressed as a physical fusion. As a model system, we have constructed several fusion protein variants in which farnesyl diphosphate synthase (FPPS) of yeast has been coupled to patchoulol synthase (PTS) of plant origin (Pogostemon cablin). Expression of the fusion proteins in S. cerevisiae...... increased the production of patchoulol, the main sesquiterpene produced by PTS, up to 2-fold. Moreover, we have demonstrated that the fusion strategy can be used in combination with traditional metabolic engineering to further increase the production of patchoulol. This simple test case of synthetic biology...

  19. Sesquiterpene lactone dermatitis in the young: is atopy a risk factor?

    DEFF Research Database (Denmark)

    Paulsen, Evy; Otkjaer, Aksel; Andersen, Klaus E

    2008-01-01

    Screening for Compositae contact allergy has documented fairly high prevalence in adults, and recent studies indicate that the allergy may be more common in children than previously believed. However, detailed information on sensitization in this age group is sparse. The objective of this study...... was to present another 2 cases in children and review the literature. Screening with sesquiterpene lactone (SL) mix has shown prevalence of 0.5% and 1.8% in 2 studies, while screening with 2 different Compositae mixes detected 4.2% and 2.6% positives among children and adolescents. All individual case reports...

  20. Patch testing with thin-layer chromatograms of chamomile tea in patients allergic to sesquiterpene lactones.

    Science.gov (United States)

    Lundh, Kerstin; Gruvberger, Birgitta; Möller, Halvor; Persson, Lena; Hindsén, Monica; Zimerson, Erik; Svensson, Ake; Bruze, Magnus

    2007-10-01

    Patients with contact allergy to sesquiterpene lactones (SLs) are usually hypersensitive to Asteraceae plant products such as herbal teas. The objective of this study was to show sensitizers in chamomile tea by patch testing with thin-layer chromatograms. Tea made from German chamomile was separated by thin-layer chromatography. Strips of the thin-layer chromatograms were used for patch testing SL-positive patients. 15 (43%) of 35 patients tested positively to 1 or more spots on the thin-layer chromatogram, with many individual reaction patterns. Patch testing with thin-layer chromatograms of German chamomile tea showed the presence of several allergens.

  1. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY USE IN ANALYSIS OF SESQUITERPENIC LACTONES OF LAURUS NOBILIS

    Directory of Open Access Journals (Sweden)

    S. P. Senchenko

    2015-01-01

    Full Text Available We have developed a methodology of quantitative determination of sesquiterpenic lactones (costunolide and dehydrocostuslactone in leaves of Laurus nobilis using reversed phase HPLC. We have determined optimal conditions for the substances under study extraction from active parts. Using the developed methods we have established that costunolide and dehydrocostuslactone content in Laurus nobilis samples in terms of absolutely dry active parts is 0.99 and 0.26% correspondingly.

  2. Bisdioxycalamenene: A Bis-Sesquiterpene from the Soft Coral Rhytisma fulvum fulvum

    Directory of Open Access Journals (Sweden)

    Yuval J. Trifman

    2016-02-01

    Full Text Available A dichloromethane extract of the soft coral Rhytisma fulvum fulvum collected in Madagascar afforded a novel compound possessing an unprecedented pentacyclic skeleton, bisdioxycalamenene (1, as well as seven known sesquiterpenes. The structures of the compounds were elucidated using 1D and 2D NMR techniques, as well as high-resolution mass spectrometry. The absolute configuration of 1 was determined using X-ray diffraction analysis and anomalous dispersion effects. The structure elucidation and a possible biogenesis of the compound are discussed.

  3. A síntese dos sesquiterpenos baquenolidas The synthesis of the bakkenolide sesquiterpenes

    Directory of Open Access Journals (Sweden)

    Timothy John Brocksom

    2008-01-01

    Full Text Available The bakkenolide group of sesquiterpenes has been a focal point of synthetic efforts amongst several research laboratories all linked to our common origins at the Chemistry Institute at the State University in São Paulo. In this review we would like to present a historical perspective of this research, with unpublished background information, and a definitive retrosynthetic analysis of these molecules. This chemistry spans the time period from 1972 to 2006, and traces the initial steps with Prof. Dr. Helena Ferraz to the present day.

  4. A New Cadinane Sesquiterpene from the Marine Brown Alga Dictyopteris divaricata

    Directory of Open Access Journals (Sweden)

    Qin-Zhao Xue

    2009-06-01

    Full Text Available A sample of the marine brown alga D. divaricata collected off the coast of Yantai (P.R. China was dried, powdered, and extracted with the mixture of CHCl3 and MeOH (1:1, v/v. By a combination of silica gel and Sephadex LH-20 column chromatography and preparative TLC, a new cadinane sesquiterpene 1,4-epoxymuurolan-5β-ol (1 was isolated from this species. Its structure was established by detailed MS and NMR spectroscopic analysis, as well as comparison with literature data.

  5. Antifouling sesquiterpene from the Indian soft coral, Sinularia kavarattiensis Alderslade and Prita

    Digital Repository Service at National Institute of Oceanography (India)

    LimnaMol, V.P.; Raveendran, T.V.; Parameswaran, P.S.; Kunnath, R.J; Sathyan, N.

    of Marine Sciences Vol. 39(2), June 2010, pp. 270-273 Antifouling sesquiterpene from the Indian soft coral, Sinularia kavarattiensis Alderslade and Prita V P Limna Mol, T V Raveendrana,*, P S Parameswaran, R J Kunnath and N Sathyan a... kavarattiensis, is an INDIAN J. MAR. SCI., VOL. 39, NO. 2, JUNE 2010 272 abundant species in the Gulf of Mannar region and Lakshadweep islands. The only reported chemical examination of S. kavarattiensis is the isolation of methyl (1′E, 5′Z)-5...

  6. Alkaloids and Sesquiterpenes from the South China Sea Gorgonian Echinogorgia pseudossapo

    Directory of Open Access Journals (Sweden)

    Shu-Hua Qi

    2011-11-01

    Full Text Available Five zoanthoxanthin alkaloids (1–5 and four sesquiterpenes (6–9 were isolated from the South China Sea gorgonian Echinogorgia pseudossapo. Their structures were determined on the bases of extensive spectroscopic analyses, including 1D and 2D NMR data. Among them, pseudozoanthoxanthins III and IV (1–2, 8-hydroxy-6β-methoxy-14-oxooplop-6,12-olide (6 and 3β-methoxyguaian-10(14-en-2β-ol (7 were new, 1 and 3 showed mild anti-HSV-1 activity, and 7 showed significant antilarval activity towards Balanus amphitrite larvae.

  7. Oppositines A and B, Sesquiterpene Pyridine Alkaloids from a Sri Lankan Pleurostylia opposita

    Science.gov (United States)

    Whitson, Emily L.; Mala, S.M.V. Damayanthi; Veltri, Charles. A.; Bugni, Tim S.; de Silva, E. Dilip; Ireland, Chris M.

    2008-01-01

    Two new sesquiterpene pyridine alkaloids, oppositines A (1) and B (2), have been isolated from the plant, Pleurostylia opposita (Celastraceae), collected in Sri Lanka. The compounds were isolated and purified by solvent/solvent partitioning, column chromatography and HPLC. Their structures were assigned on the basis of extensive 1D and 2D NMR studies as well as analysis by HRESIMS. Oppositines A (1) and B (2) showed moderate cytotoxicity against HCT116 cell lines with EC50 values of 27 ± 2 and 26 ± 3 μM, respectively. PMID:17190474

  8. Chemical constituents of the mangrove-associated fungus Capnodium sp. SZ-F22. A new eremophilane sesquiterpene.

    Science.gov (United States)

    He, Haibing; Ma, Zhongjun; Wang, Qianqian; Liu, Yu; Xu, Hualin

    2016-07-01

    A new eremophilane sesquiterpene, capnodiumone (1), along with five known eremophilane sesquiterpenes (2-6) and eight other compounds (7-14), have been isolated from a mangrove-associated fungus Capnodium sp. SZ-F22. The chemical structures were elucidated on the basis of extensive spectroscopic analysis. The broth extract of the fungus exhibited a good inhibitory effect on the mycelium growth against Fusarium graminearum at 100 μg/mL, however, all the 14 compounds showed no expected antifungal activity. The probable reasons were discussed.

  9. Sesquiterpenes from Curcuma wenyujin with their inhibitory activities on nitric oxide production in RAW 264.7 cells.

    Science.gov (United States)

    Gao, Suyu; Xia, Guiyang; Wang, Liqing; Zhou, Li; Zhao, Feng; Huang, Jian; Chen, Lixia

    2017-03-01

    One new sesquiterpene, 7α,11-epoxy-6α-hydroxy-carabrane-4,8-dione, along with 10 known ones were isolated from the essential oil of Curcuma wenyujin Y.H. Chen et C. Ling. Their structures were established based on extensive spectroscopic analysis. The absolute configuration of compound 1 was determined by the CD analysis of the insitu formed [Rh 2 (OCOCF 3 ) 4 ] complex, and the CD data analysis based on the octane rule of cyclohexanone. The inhibitory effects of these sesquiterpenes on nitric oxide production in lipopolysaccharide-activated macrophages were also evaluated. Furthermore, the biosynthesis pathway of the isolated compounds was proposed.

  10. Biosynthesis of the irregular monoterpene artemisia ketone, the sesquiterpene germacrene D and other isoprenoids in Tanacetum vulgare L. (Asteraceae).

    Science.gov (United States)

    Umlauf, Dirk; Zapp, Josef; Becker, Hans; Adam, Klaus Peter

    2004-09-01

    The incorporation of [1-13C]-labeled glucose into the irregular monoterpene artemisia ketone, the regular monoterpenes camphor and beta-thujone, the sesquiterpene germacrene D, the diterpene trans-phytol and beta-sitosterol and isofucosterol has been studied in axenic cultures of Tanacetum vulgare L. (Asteraceae). Quantitative 13C NMR spectroscopic analysis of the resulting labeling patterns showed that the isoprene units of the monoterpenes and the diterpene are formed via the methylerythritol phosphate (MEP) pathway, whereas the isoprene building blocks of the sesquiterpene and the sterols originate from the mevalonic acid (MVA) pathway. Copyright 2004 Elsevier Ltd.

  11. Heliolactone, a non-sesquiterpene lactone germination stimulant for root parasitic weeds from sunflower.

    Science.gov (United States)

    Ueno, Kotomi; Furumoto, Toshio; Umeda, Shuhei; Mizutani, Masaharu; Takikawa, Hirosato; Batchvarova, Rossitza; Sugimoto, Yukihiro

    2014-12-01

    Root exudates of sunflower (Helianthus annuus L.) line 2607A induced germination of seeds of root parasitic weeds Striga hermonthica, Orobanche cumana, Orobanche minor, Orobanche crenata, and Phelipanche aegyptiaca. Bioassay-guided purification led to the isolation of a germination stimulant designated as heliolactone. FT-MS analysis indicated a molecular formula of C20H24O6. Detailed NMR spectroscopic studies established a methylfuranone group, a common structural component of strigolactones connected to a methyl ester of a C14 carboxylic acid via an enol ether bridge. The cyclohexenone ring is identical to that of 3-oxo-α-ionol and the other part of the molecule corresponds to an oxidized carlactone at C-19. It is a carlactone-type molecule and functions as a germination stimulant for seeds of root parasitic weeds. Heliolactone induced seed germination of the above mentioned root parasitic weeds, while dehydrocostus lactone and costunolide, sesquiterpene lactones isolated from sunflower root exudates, were effective only on O. cumana and O. minor. Heliolactone production in aquacultures increased when sunflower seedlings were grown hydroponically in tap water and decreased on supplementation of the culture with either phosphorus or nitrogen. Costunolide, on the other hand, was detected at a higher concentration in well-nourished medium as opposed to nutrient-deficient media, thus suggesting a contrasting contribution of heliolactone and the sesquiterpene lactone to the germination of O. cumana under different soil fertility levels. Copyright © 2014 Elsevier Ltd. All rights reserved.

  12. Sesquiterpenes from the seeds of Guarea guidonia (Meliaceae); Sesquiterpenos de sementes de Guarea guidonia (Meliaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Soares, Luzinatia Ramos [Departamento de Quimica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS (Brazil); Silva, Alene Cortes de Queiroz e; Freire, Talita Vilalva; Garcez, Fernanda Rodrigues; Garcez, Walmir Silva [Departamento de Quimica, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS (Brazil)

    2012-07-01

    Two new sesquiterpenes 6{alpha}-ethoxyeudesm-4(15)-en-1{beta}-ol (1) and (7R{sup *})-5-epi-opposit-4(15)-ene-1{beta},7-diol (7) were isolated from the seeds of a specimen of Guarea guidonia (Meliaceae). Five other known sesquiterpenes, eudesm-4(15)-ene-1{beta},6a-diol (2), 5-epi-eudesm-4(15)-ene-1{beta},6{beta}-diol (3), eudesm-4(15)-ene-1{beta},5a-diol (4), eudesm-4(15),7-dien-1{beta}-ol (5) and (7R{sup *})-opposit-4(15)-ene-1{beta},7-diol (6), were also isolated in this work. Their structures were determined on the basis of spectral data (MS, IR, and NMR-1D and 2D) and subsequent comparisons with the literature. The substances 3, 4 and 6 are reported for the first time in Meliaceae and 5 for the first time in the genus Guarea. (author)

  13. Hypo-pigmenting effect of sesquiterpenes from Inula britannica in B16 melanoma cells.

    Science.gov (United States)

    Choo, Soo-Jin; Ryoo, In-Ja; Kim, Kwan Chul; Na, Minkyun; Jang, Jae-Hyuk; Ahn, Jong Seog; Yoo, Ick-Dong

    2014-05-01

    During the course of screens to identify anti-melanogenic agents from natural resources, we found that the methanol extract of the dried flower of Inula britannica L. inhibited melanin synthesis in cultured melanoma cells stimulated with 3-isobutyl-1-methylxanthine (IBMX). A bioassay-guided isolation of the chloroform fraction of the I. britannica using an in vitro melanogenesis inhibition assay led to the isolation of sesquiterpenes, 1-O-acetylbritannilactone (1), britannilactone (2) and neobritannilactone B (3). Compounds 1 and 2 significantly reduced melanin production in a dose-dependent manner with IC50 values of 13.3 and 15.5 μM, respectively, whereas compound 3 was found to be cytotoxic. Compound 1 also inhibited the tyrosinase activity only in cell based-systems. Western blot analysis indicated that compound 1 inhibited melanogenesis by activating extracellular signal-regulated kinase (ERK) and Akt signaling and also inhibiting cAMP related binding protein, which regulates its downstream pathway, including tyrosinase, tyrosinase related protein-1 and TRP-2. These results demonstrated that compound 1, a major sesquiterpene from the flowers of I. britannica, exhibited anti-melanogenic activity by suppression of tyrosinase expression via ERK and Akt signaling. Taken together, our results suggest that these compounds may act as potent natural skin-lightening agents.

  14. CYP264B1 from Sorangium cellulosum So ce56: a fascinating norisoprenoid and sesquiterpene hydroxylase.

    Science.gov (United States)

    Ly, Thuy T B; Khatri, Yogan; Zapp, Josef; Hutter, Michael C; Bernhardt, Rita

    2012-07-01

    Many terpenes and terpenoid compounds are known as bioactive substances with desirable fragrance and medicinal activities. Modification of such compounds to yield new derivatives with desired properties is particularly attractive. Cytochrome P450 monooxygenases are potential enzymes for these reactions due to their capability of performing different reactions on a variety of substrates. We report here the characterization of CYP264B1 from Sorangium cellulosum So ce56 as a novel sesquiterpene hydroxylase. CYP264B1 was able to convert various sesquiterpenes including nootkatone and norisoprenoids (α-ionone and β-ionone). Nootkatone, an important grapefruit aromatic sesquiterpenoid, was hydroxylated mainly at position C-13. The product has been shown to have the highest antiproliferative activity compared with other nootkatone derivatives. In addition, CYP264B1 was found to hydroxylate α- and β-ionone, important aroma compounds of floral scents, regioselectively at position C-3. The products, 3-hydroxy-β-ionone and 13-hydroxy-nootkatone, were confirmed by (1)H and (13)C NMR. The kinetics of the product formation was analyzed by high-performance liquid chromatography, and the K ( m ) and k (cat) values were calculated. The results of docking α-/β-ionone and nootkatone into a homology model of CYP264B1 revealed insights into the structural basis of these selective hydroxylations.

  15. Vitis vinifera terpenoid cyclases: functional identification of two sesquiterpene synthase cDNAs encoding (+)-valencene synthase and (-)-germacrene D synthase and expression of mono- and sesquiterpene synthases in grapevine flowers and berries.

    Science.gov (United States)

    Lücker, Joost; Bowen, Pat; Bohlmann, Jörg

    2004-10-01

    Valencene is a volatile sesquiterpene emitted from flowers of grapevine, Vitis vinifera L. A full-length cDNA from the cultivar Gewürztraminer was functionally expressed in Escherichia coli and found to encode valencene synthase (VvVal). The two major products formed by recombinant VvVal enzyme activity with farnesyl diphosphate (FPP) as substrate are (+)-valencene and (-)-7-epi-alpha-selinene. Grapevine valencene synthase is closely related to a second sesquiterpene synthase from this species, (-)-germacrene D synthase (VvGerD). VvVal and VvGerD cDNA probes revealed strong signals in Northern hybridizations with RNA isolated from grapevine flower buds. Transcript levels were lower in open pre-anthesis flowers, flowers after anthesis, or at early onset of fruit development. Similar results were obtained using a third probe, (-)-alpha-terpineol synthase, a monoterpenol synthase. Sesquiterpene synthase and monoterpene synthase transcripts were not detected in the mesocarp and exocarp during early stages of fruit development, but transcripts hybridizing with VvVal appeared during late ripening of the berries. Sesquiterpene synthase transcripts were also detected in young seeds.

  16. Um estudo numa IPSS

    OpenAIRE

    Casadinho, Anabela Gertrudes Vaqueirinho Bilro

    2014-01-01

    Atualmente, o sucesso das organizações está dependente em grande parte do potencial humano lá existente, ou seja, são esperados resultados mais positivos quando existem ambientes saudáveis de participação, colaboração e cooperação. Este estudo foi inicialmente pensado para investigar o problema das relações com o trabalho em contexto organizacional. A hipótese considerada, é de existirem diferentes modos de relação com o trabalho e que esta se articula com as pertenças sociais. Desta forma...

  17. CYTOTOXICITY OF FLAVONOIDS AND SESQUITERPENE LACTONES FROM ARNICA SPECIES AGAINST THE GLC(4) AND THE COLO-320 CELL-LINES

    NARCIS (Netherlands)

    WOERDENBAG, HJ; MERFORT, [No Value; PASSREITER, CM; SCHMIDT, TJ; WILLUHN, G; VANUDEN, W; PRAS, N; KAMPINGA, HH; KONINGS, AWT

    1994-01-01

    The cytotoxicity of 21 flavonoids and 5 sesquiterpene lactones, as present in Arnica species, was studied in GLC(4), a human small cell lung carcinoma cell line, and in COLO 320, a human colorectal cancer cell line, using the microculture tetrazolium (MTT) assay. Following continuous incubation,

  18. Effects of sesquiterpene, flavonoid and coumarin types of compounds from Artemisia annua L. on production of mediators of angiogenesis

    Czech Academy of Sciences Publication Activity Database

    Zhu, X. X.; Yang, L.; Li, Y. J.; Zhang, D.; Chen, Y.; Kostecká, Petra; Kmoníčková, Eva; Zídek, Zdeněk

    2013-01-01

    Roč. 65, č. 2 (2013), s. 410-420 ISSN 1734-1140 R&D Projects: GA MŠk(CZ) ME10116 Institutional support: RVO:68378041 Keywords : sesquiterpenes * flavonoids * immunosuppression Subject RIV: FR - Pharmacology ; Medidal Chemistry Impact factor: 2.165, year: 2013

  19. Artemisinin-derived sesquiterpene lactones as potential antitumour compounds : Cytotoxic action against bone marrow and tumour cells

    NARCIS (Netherlands)

    Beekman, AC; Wierenga, PK; Woerdenbag, HJ; Van Uden, W; Pras, N; Konings, AWT; El-Feraly, FS; Galal, AM; Wikstrom, HV

    1998-01-01

    We determined the in vitro cytotoxic activity of the sesquiterpene lactone endoperoxide artemisinin (1) and some chemically prepared derivatives, which have been found to display cytotoxicity to cloned murine Ehrlich ascites tumour (EAT) cells and human HeLa cells and against murine bone marrow

  20. Studies on the expression of sesquiterpene synthases using promoter-β-glucuronidase fusions in transgenic Artemisia annua L.

    Directory of Open Access Journals (Sweden)

    Hongzhen Wang

    Full Text Available In order to better understand the influence of sesquiterpene synthases on artemisinin yield in Artemisia annua, the expression of some sesquiterpene synthases has been studied using transgenic plants expressing promoter-GUS fusions. The cloned promoter sequences were 923, 1182 and 1510 bp for β-caryophyllene (CPS, epi-cedrol (ECS and β-farnesene (FS synthase, respectively. Prediction of cis-acting regulatory elements showed that the promoters are involved in complex regulation of expression. Transgenic A. annua plants carrying promoter-GUS fusions were studied to elucidate the expression pattern of the three sesquiterpene synthases and compared to the previously studied promoter of amorpha-4,11-diene synthase (ADS, a key enzyme of artemisinin biosynthesis. The CPS and ECS promoters were active in T-shaped trichomes of leaves and stems, basal bracts of flower buds and also in some florets cells but not in glandular secretory trichome while FS promoter activity was only observed in leaf cells and trichomes of transgenic shoots. ADS, CPS, ECS and FS transcripts were induced by wounding in a time depended manner. The four sesquiterpene synthases may be involved in responsiveness of A. annua to herbivory. Methyl jasmonate treatment triggered activation of the promoters of all four sesquiterpene synthases in a time depended manner. Southern blot result showed that the GUS gene was inserted into genomic DNA of transgenic lines as a single copy or two copies. The relative amounts of CPS and ECS as well as germacrene A synthase (GAS transcripts are much lower than that of ADS transcript. Consequently, down-regulation of the expression of the CPS, ECS or GAS gene may not improve artemsinin yield. However, blocking the expression of FS may have effects on artemisinin production.

  1. Studies on the expression of sesquiterpene synthases using promoter-β-glucuronidase fusions in transgenic Artemisia annua L.

    Science.gov (United States)

    Wang, Hongzhen; Han, Junli; Kanagarajan, Selvaraju; Lundgren, Anneli; Brodelius, Peter E

    2013-01-01

    In order to better understand the influence of sesquiterpene synthases on artemisinin yield in Artemisia annua, the expression of some sesquiterpene synthases has been studied using transgenic plants expressing promoter-GUS fusions. The cloned promoter sequences were 923, 1182 and 1510 bp for β-caryophyllene (CPS), epi-cedrol (ECS) and β-farnesene (FS) synthase, respectively. Prediction of cis-acting regulatory elements showed that the promoters are involved in complex regulation of expression. Transgenic A. annua plants carrying promoter-GUS fusions were studied to elucidate the expression pattern of the three sesquiterpene synthases and compared to the previously studied promoter of amorpha-4,11-diene synthase (ADS), a key enzyme of artemisinin biosynthesis. The CPS and ECS promoters were active in T-shaped trichomes of leaves and stems, basal bracts of flower buds and also in some florets cells but not in glandular secretory trichome while FS promoter activity was only observed in leaf cells and trichomes of transgenic shoots. ADS, CPS, ECS and FS transcripts were induced by wounding in a time depended manner. The four sesquiterpene synthases may be involved in responsiveness of A. annua to herbivory. Methyl jasmonate treatment triggered activation of the promoters of all four sesquiterpene synthases in a time depended manner. Southern blot result showed that the GUS gene was inserted into genomic DNA of transgenic lines as a single copy or two copies. The relative amounts of CPS and ECS as well as germacrene A synthase (GAS) transcripts are much lower than that of ADS transcript. Consequently, down-regulation of the expression of the CPS, ECS or GAS gene may not improve artemsinin yield. However, blocking the expression of FS may have effects on artemisinin production.

  2. In Vitro antileishmania activity of sesquiterpene-rich essential oils from Nectandra species.

    Science.gov (United States)

    Bosquiroli, Lauriane Serpa Silva; Dos Santos Ferreira, Ana Caroline; Farias, Katyuce Souza; da Costa, Eduarda Carneiro; Matos, Maria de Fátima Cepa; Kadri, Mônica Cristina Toffoli; Rizk, Yasmin Silva; Alves, Flávio Macedo; Perdomo, Renata Trentin; Carollo, Carlos Alexandre; Pinto de Arruda, Carla Cardozo

    2017-12-01

    New antileishmanias are needed because of toxicity, high cost and resistance problems associated with available drugs. Nectandra (Lauraceae) produces several classes of compounds but its essential oil has not previously been reported to have antileishmania activity. We evaluated the cytotoxicity and antileishmania activity of essential oils from Nectandra amazonum Nees, N. gardneri Meisn., N. hihua (Ruiz & Pav.) Rohwer and N. megapotamica (Spreng.) Mez. Nectandra oils were extracted from stem bark/leaves by hydrodistillation and compounds were identified by GC-MS. Oils were tested against Leishmania infantum and L. amazonensis intracellular amastigotes and nitric oxide production was evaluated. Cytotoxicity was achieved on NIH/3T3 and J774.A1 cells for the selectivity index (SI). Nectandra gardneri was active against L. infantum and L. amazonensis (IC 50  =  2.7 ± 1.3/2.1 ± 1.06 μg/mL) and contained 85.4% sesquiterpenes, of which 58.2% was intermediol. Besides low cytotoxicity (SI >11.3), N. gardneri induced a significant increase in NO production by L. infantum-infected macrophages. Nectandra hihua had the best activity on L. infantum amastigotes (IC 50  =  0.2 ± 1.1 μg/mL). This oil was 89.0% sesquiterpenes, with 28.1% bicyclogermacrene. The two specimens of N. megapotamica had different activities on amastigotes. The one richer in sesquiterpenes (49.9%) was active against both species (IC 50  =  12.5 ± 1.4/21.3 ± 1.2) and had phenylpropanoid E-asarone as the main compound (42.4%). Nectandra amazonum showed moderate activity on both the species (IC 50  =  31.9 ± 2.0/22.1 ± 1.3 μg/mL) and low selectivity (0.9 2.6), probably due to the major presence of β-caryophyllene (28.5%). Our data identify compounds that can now be isolated and used for the development of new antileishmanias.

  3. Hygroscopicity of secondary organic aerosols formed by oxidation of cycloalkenes, monoterpenes, sesquiterpenes, and related compounds

    Directory of Open Access Journals (Sweden)

    V. Varutbangkul

    2006-01-01

    Full Text Available A series of experiments has been conducted in the Caltech indoor smog chamber facility to investigate the water uptake properties of aerosol formed by oxidation of various organic precursors. Secondary organic aerosol (SOA from simple and substituted cycloalkenes (C5-C8 is produced in dark ozonolysis experiments in a dry chamber (RH~5%. Biogenic SOA from monoterpenes, sesquiterpenes, and oxygenated terpenes is formed by photooxidation in a humid chamber (~50% RH. Using the hygroscopicity tandem differential mobility analyzer (HTDMA, we measure the diameter-based hygroscopic growth factor (GF of the SOA as a function of time and relative humidity. All SOA studied is found to be slightly hygroscopic, with smaller water uptake than that of typical inorganic aerosol substances. The aerosol water uptake increases with time early in the experiments for the cycloalkene SOA, but decreases with time for the sesquiterpene SOA. This behavior could indicate competing effects between the formation of more highly oxidized polar compounds (more hygroscopic, and formation of longer-chained oligomers (less hygroscopic. All SOA also exhibit a smooth water uptake with RH with no deliquescence or efflorescence. The water uptake curves are found to be fitted well with an empirical three-parameter functional form. The measured pure organic GF values at 85% RH are between 1.09–1.16 for SOA from ozonolysis of cycloalkenes, 1.01–1.04 for sesquiterpene photooxidation SOA, and 1.06–1.10 for the monoterpene and oxygenated terpene SOA. The GF of pure SOA (GForg in experiments in which inorganic seed aerosol is used is determined by assuming volume-weighted water uptake (Zdanovskii-Stokes-Robinson or 'ZSR' approach and using the size-resolved organic mass fraction measured by the Aerodyne Aerosol Mass Spectrometer. Knowing the water content associated with the inorganic fraction yields GForg values. However, for each precursor, the GForg values computed from different

  4. C21-steroidal glycosides and sesquiterpenes from the roots of Cynanchum bungei and their inhibitory activities against the proliferation of B and T lymphocytes.

    Science.gov (United States)

    Qin, Jun-Jun; Chen, Xi; Lin, Ze-Min; Xu, Yan-Sheng; Li, Yi-Ming; Zuo, Jian-Ping; Zhao, Wei-Min

    2018-01-01

    Phytochemical investigation of the roots of Cynanchum bungei Decne (Asclepiadaceae) led to the elucidation of seven C 21 -steroidal glycosides (1-7) including three new compounds (1-3), named cynabungosides A-C, one new eudesmane-type sesquiterpene (8), named cynabungone, and one new humulane-type sesquiterpene (9), named cynabungolide. Their structures were elucidated on the basis of extensive spectroscopic analysis. The absolute configurations of 8 and 9 were defined unequivocally by ECD analysis and X-ray crystallography, respectively. A putative biosynthetic pathway of humulane-type sesquiterpenes 9 and 10 is proposed. Copyright © 2017 Elsevier B.V. All rights reserved.

  5. Bioactive constituents of Chinese natural medicines. I. New sesquiterpene ketones with vasorelaxant effect from Chinese moxa, the processed leaves of Artemisia argyi Levl. et Vant.: moxartenone and moxartenolide.

    Science.gov (United States)

    Yoshikawa, M; Shimada, H; Matsuda, H; Yamahara, J; Murakami, N

    1996-09-01

    Two new sesquiterpene ketones, moxartenone and moxartenolide, and three octadecadienoic acids were isolated from Chinese moxa, the processed leaves of Artemisia argyi LEVL. et VANT., together with two sesquiterpenes, five triterpenes, two phenyl propanoids and three polyoxyflavones. The chemical structures of new sesquiterpenes, moxartenone, moxartenolide, and octadecadienoic acids were determined on the basis of chemical and physiochemical evidence. Moxartenolide was found to inhibit the contractions induced by a high concentration of K+, by norepinephrine, and by serotonin in isolated aortic strips of rat, while moxartenone showed little activity.

  6. New Bisabolane-Type Sesquiterpenes from the Aerial Parts of Lippia dulcis.

    Science.gov (United States)

    Ono, Masateru; Morinaga, Hiroaki; Masuoka, Chikako; Ikeda, Tsuyoshi; Okawa, Masafumi; Kinjo, Junei; Nohara, Toshihiro

    2005-09-01

    Two new bisabolane-type sesquiterpenes, lippidulcine A (3) and epilippidulcine A (4), have been isolated from the aerial parts of Lippia dulcis TREV. along with five known flavonoids, cirsimaritin (5), salvigenin (6), eupatorin (7), 5-hydroxy-6,7,3',4'-tetramethoxyflavone (8) and 5,3'-dihydroxy-6,7,4',5'-tetramethoxyflavone (9), three known phenylethanoid glycosides, decaffeoylverbascoside (10), acteoside (11) and isoacteoside (12), and two known iridoid glucosides, 8-epiloganin (13) and lamiide (14). Their chemical structures have been determined on the basis of spectroscopic data. Among them, 5, 7, and 9 exhibited almost the same activity as that of alpha-tocopherol, and 10-12 were identified as stronger antioxidants than alpha-tocopherol using the ferric thiocyanate method.

  7. Five rare C32sesquiterpene lactone dimers with anti-inflammation activity from Vladimiria souliei.

    Science.gov (United States)

    Wu, Zhi-Li; Wang, Jin-Xin; Chen, Li-Ping; Dong, Hong-Yuan; Li, Hui-Liang; Zhang, Wei-Dong

    2018-03-01

    Five rare sesquiterpene lactone dimers, vlasouliolides E-I, were isolated from Vladimiria souliei. Their chemical structures were elucidated by spectroscopic analysis. Furthermore, 2 and 4 were unambiguously confirmed by Cu-Kα X-ray crystallographic analysis. Compounds 1, 2, 4 and 5 exhibited significant anti-inflammatory activity against LPS-induced NO production in RAW 264.7 cells with IC 50 values of 1.88, 4.89, 7.24 and 2.46μM, respectively. Additionally, compounds 1 and 2 were revealed with potent inhibitory activity of the phosphorylation progress of NF-κB P65. Copyright © 2018 Elsevier B.V. All rights reserved.

  8. Sesquiterpene Quinones/Hydroquinones from the Marine Sponge Spongia pertusa Esper.

    Science.gov (United States)

    Li, Jing; Gu, Bin-Bin; Sun, Fan; Xu, Jian-Rong; Jiao, Wei-Hua; Yu, Hao-Bing; Han, Bing-Nan; Yang, Fan; Zhang, Xi-Chun; Lin, Hou-Wen

    2017-05-26

    Nine new sesquiterpene quinones/hydroquinones (1-7, 10, and 12), three solvent-generated artifacts (8, 9, and 11), and three known compounds, 5-epi-smenospongine (13), smenospongine (14), and smenospongiadine (15), were isolated from the marine sponge Spongia pertusa Esper. The planar structures of the new compounds were elucidated on the basis of spectroscopic analyses. Their absolute configurations were determined by comparison between the calculated and experimental ECD spectra. In the cytotoxicity bioassay, compounds 13-15 exhibited activities against the human cancer cell lines U937, HeLa, and HepG2, with most potent cytotoxicities to U937 cells with IC 50 values of 2.8, 1.5, and 0.6 μM, respectively. In addition, compound 6 displayed CDK-2 affinity with a K d value of 4.8 μM in a surface plasmon resonance assay.

  9. Sesquiterpene derivatives from marine sponge Smenospongia cerebriformis and their anti-inflammatory activity.

    Science.gov (United States)

    Kiem, Phan Van; Huyen, Le Thi; Hang, Dan Thuy; Nhiem, Nguyen Xuan; Tai, Bui Huu; Anh, Hoang Le Tuan; Cuong, Pham Van; Quang, Tran Hong; Minh, Chau Van; Dau, Nguyen Van; Kim, Young-A; Subedi, Lalita; Kim, Sun Yeou; Kim, Seung Hyun

    2017-04-01

    Using various chromatographic methods, five new sesquiterpene derivatives named smenohaimiens A-E (1-5) and five known, 19-hydroxy-polyfibrospongol B (6), ilimaquinone (7), dictyoceratin C (8), polyfibrospongol A (9), and polyfibrospongol B (10) were isolated from the marine sponge Smenospongia cerebriformis Duchassaing & Michelotti, 1864. Their structures were assigned by 1D, 2D NMR spectroscopic analysis, HR ESI MS, and calculations of the electron circular dichroism spectra. All compounds were evaluated for the inhibitory activity against NO production in lipopolysaccharide-stimulated in BV2 microglia cells. As the results, compound 7 significantly inhibited NO production with the IC 50 value of 10.40±1.28µM. The remaining compounds showed moderate inhibitory NO production activities with IC 50 values ranging from 24.37 to 30.43µM. Copyright © 2017 Elsevier Ltd. All rights reserved.

  10. Stelliosphaerols A and B, Sesquiterpene-Polyol Conjugates from an Ecuadorian Fungal Endophyte.

    Science.gov (United States)

    Forcina, Giovanni C; Castro, Amaya; Bokesch, Heidi R; Spakowicz, Daniel J; Legaspi, Michelle E; Kucera, Kaury; Villota, Stephany; Narváez-Trujillo, Alexandra; McMahon, James B; Gustafson, Kirk R; Strobel, Scott A

    2015-12-24

    Endophytic fungi are plant tissue-associated fungi that represent a rich resource of unexplored biological and chemical diversity. As part of an ongoing effort to characterize Amazon rainforest-derived endophytes, numerous fungi were isolated and cultured from plants collected in the Yasuní National Park in Ecuador. Of these samples, phylogenetic and morphological data revealed a previously undescribed fungus in the order Pleosporales that was cultured from the tropical tree Duroia hirsuta. Extracts from this fungal isolate displayed activity against Staphylococcus aureus and were thus subjected to detailed chemical studies. Two compounds with modest antibacterial activity were isolated, and their structures were elucidated using a combination of NMR spectroscopic analysis, LC-MS studies, and chemical degradation. These efforts led to the identification of stelliosphaerols A (1) and B (2), new sesquiterpene-polyol conjugates that are responsible, at least in part, for the S. aureus inhibitory activity of the fungal extract.

  11. Synthesis and Neurotrophic Activity Studies of Illicium Sesquiterpene Natural Product Analogues.

    Science.gov (United States)

    Richers, Johannes; Pöthig, Alexander; Herdtweck, Eberhardt; Sippel, Claudia; Hausch, Felix; Tiefenbacher, Konrad

    2017-03-02

    Neurotrophic natural products hold potential as privileged structures for the development of therapeutic agents against neurodegeneration. However, only a few studies have been conducted to investigate a common pharmacophoric motif and structure-activity relationships (SARs). Here, an investigation of structurally more simple analogues of neurotrophic sesquiterpenes of the illicium family is presented. A concise synthetic route enables preparation of the carbon framework of (±)-Merrilactone A and (±)-Anislactone A/B on a gram scale. This has allowed access to a series of structural analogues by modification of the core structure, including variation of oxidation levels and alteration of functional groups. In total, 15 derivatives of the natural products have been synthesized and tested for their neurite outgrowth activities. Our studies indicate that the promising biological activity can be retained by structurally simpler natural product analogues, which are accessible by a straightforward synthetic route. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. Antiphytoviral Activity of Sesquiterpene-Rich Essential Oils from Four Croatian Teucrium Species

    Directory of Open Access Journals (Sweden)

    Franko Burčul

    2011-09-01

    Full Text Available The purpose of this study was to compare the essential oil profiles of four Croatian Teucrium species (Lamiaceae, as determined by GC and GC/MS, with their antiphytoviral efficiency. A phytochemical analysis showed that T. polium, T. flavum, T. montanum and T. chamaedrys are characterized by similar essential oil compositions. The investigated oils are characterized by a high proportion of the sesquiterpene hydrocarbons β-caryophyllene (7.1–52.0% and germacrene D (8.7–17.0%. Other important components were β-pinene from T. montanum and α-pinene from T. flavum. The investigated essential oils were proved to reduce lesion number in the local host Chenopodium quinoa Willd. infected with Cucumber Mosaic Virus (CMV, with reductions of 41.4%, 22.9%, 44.3% and 25.7%, respectively.

  13. Covalent Modification of Human Serum Albumin by the Natural Sesquiterpene Lactone Parthenolide

    Directory of Open Access Journals (Sweden)

    Michael Plöger

    2015-04-01

    Full Text Available The reactivity of parthenolide (PRT, a natural sesquiterpene lactone from Tanacetum parthenium (Asteraceae, with human serum albumin (HSA was studied by UHPLC/+ESI-QqTOF MS analysis after tryptic digestion of albumin samples after incubation with this compound. It was found that the single free cysteine residue, C34, of HSA (0.6 mM reacted readily with PRT when incubated at approximately 13-fold excess of PRT (8 mM. Time-course studies with PRT and its 11β,13-dihydro derivative at equimolar ratios of the reactants revealed that PRT under the chosen conditions reacts preferably with C34 and does so exclusively via its α-methylene-γ-lactone moiety, while the epoxide structure is not involved in the reaction.

  14. Distinct sesquiterpene pyridine alkaloids from in Salvadoran and Peruvian Celastraceae species.

    Science.gov (United States)

    Callies, Oliver; Núñez, Marvin J; Perestelo, Nayra R; Reyes, Carolina P; Torres-Romero, David; Jiménez, Ignacio A; Bazzocchi, Isabel L

    2017-10-01

    As part of a bioprospecting program aimed at the discovery of undescribed natural products from Salvadoran and Peruvian flora, the phytochemical investigations of four Celastraceae species, Celastrus vulcanicola, Maytenus segoviarum, Maytenus jeslkii, and Maytenus cuzcoina, were performed. The current study reports the isolation and structural characterization of five previously undescribed macrolide sesquiterpene pyridine alkaloids, named vulcanicoline-A, cuzcoinine, vulcanicoline-B, jelskiine, and vulcanicoline-C, along with sixteen known alkaloids. The structures of the alkaloids were established by spectrometric and extensive 1D and 2D NMR spectroscopic analysis, including COSY, HSQC, HMBC, and ROESY experiments. The absolute configurations of alkaloids were proposed based on optical rotation sign, and biogenetic considerations. This study represents the first phytochemical analysis of Maytenus segoviarum. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Anti-Trypanosomatid Elemanolide Sesquiterpene Lactones from Vernonia lasiopus O. Hoffm

    Directory of Open Access Journals (Sweden)

    Njogu M. Kimani

    2017-04-01

    Full Text Available Sleeping sickness or human African trypanosomiasis (HAT is a neglected tropical disease (NTD threatening millions of peoples’ lives with thousands infected. The disease is endemic in poorly developed regions of sub-Saharan Africa and is caused by the kinetoplastid “protozoan” parasite Trypanosoma brucei. The parasites are transmitted to humans through bites of infected tsetse flies of the genus Glossina. The few available drugs for treatment of this disease are highly toxic, difficult to administer, costly and unavailable to poor rural communities bearing the major burden of this infection. Therefore, the search for new efficacious, safe and affordable drugs is of high importance. Vernonia lasiopus O. Hoffm., an indigenous African plant of the Asteraceae family, has been extensively reported to be used ethno-medicinally as a treatment for malaria. Its crude extracts obtained with solvents of different polarity were screened in vitro for anti-protozoal activity and the dichloromethane extract was found to be particularly active against Trypanosoma brucei rhodesiense (IC50 = 0.17 µg/mL. Bioassay-guided chromatographic fractionation of the dichloromethane extract led to the isolation and identification of six elemanolide type sesquiterpene lactones: 8-desacylvernolide, vernolepin, vernomenin, vernodalol, vernodalin and 11,13-dihydrovernodalin. All these elemanolide sesquiterpene lactones showed in vitro anti-trypanosomal activity. They were also tested for cytotoxicity against mammalian cells (L6 cell line. Vernolepin, the main component in the extract, was also the most potent with an IC50 value of 0.05 µg/mL against T.b. rhodesiense trypomastigotes. This compound showed a selectivity index of 14.5, which makes it an interesting candidate for in vivo tests and determination of its mechanism of action.

  16. Evaluation of mosquitocidal activity of essential oil and sesquiterpenes from leaves of Chloroxylon swietenia DC.

    Science.gov (United States)

    Kiran, S Ravi; Devi, P Sita

    2007-07-01

    Growing awareness in using ecofriendly and biologically compatible phytoconstituents as natural insecticides and repellents for the safety of life and ecological balance led to conscientious efforts by scientists all over the world to search for alternative sources of plant derivatives for effective use as mosquitocides. Encouraged by this, the essential oil and the sesquiterpenes isolated from the leaves of Chloroxylon swietenia DC. were screened for mosquitocidal activity by fumigant toxicity against three mosquito species, Anopheles gambiae, Culex quinquefasciatus and Aedes aegypti. The essential oil had pronounced mosquitocidal activity with LD50 of 1.0, 1.2 and 1.7 x 10(-3) mg/cm(-3), respectively, for the three vector species. Furthermore, the major sesquiterpenes were tested at different doses, which again showed varying levels of toxicity. However, germacrene D performed better and proved to be the potential candidate with LD50 values of 1.8-2.8 x 10(-3) mg/cm(-3) followed by pregeijerene and geijerene. Nevertheless, the oil and the isolated compounds were particularly active against A. gambiae. The essential oil from the leaves was obtained by hydrodistillation, and the chemical composition was determined by GC and GC-MS. The main compounds identified were limonene, germacrene D, geijerene, pregeijerene, trans-beta-ocimene and methyl eugenol. The present study indicates that the oil and the isolated compounds of C. swietenia displayed remarkable mosquitocidal activity suggesting that the method could be extended for future field trials in various mosquito control programmes, and the results are compared with synthetic insecticides.

  17. Sesquiterpene lactones from Gynoxys verrucosa and their anti-MRSA activity.

    Science.gov (United States)

    Ordóñez, Paola E; Quave, Cassandra L; Reynolds, William F; Varughese, Kottayil I; Berry, Brian; Breen, Philip J; Malagón, Omar; Smeltzer, Mark S; Compadre, Cesar M

    2011-09-02

    Because of its virulence and antibiotic resistance, Staphylococcus aureus is a more formidable pathogen now than at any time since the pre-antibiotic era. In an effort to identify and develop novel antimicrobial agents with activity against this pathogen, we have examined Gynoxys verrucosa Wedd (Asteraceae), an herb used in traditional medicine in southern Ecuador for the treatment and healing of wounds. The sesquiterpene lactones leucodine (1) and dehydroleucodine (2) were extracted and purified from the aerial parts of Gynoxys verrucosa, and their structure was elucidated by spectroscopic methods and single-crystal X-ray analysis. The in vitro anti-microbial activity of Gynoxys verrucosa extracts and its purified constituents was determined against six clinical isolates including Staphylococcus aureus and Staphylococcus epidermidis strains with different drug-resistance profiles, using the microtiter broth method. Compound 1 has very low activity, while compound 2 has moderate activity with MIC(50)s between 49 and 195 μg/mL. The extract of Gynoxys verrucosa has weak activity with MIC(50)s between 908 and 3290 μg/mL. We are reporting the full assignment of the (1)H NMR and (13)C NMR of both compounds, and the crystal structure of compound 2, for the first time. Moreover, the fact that compound 2 has antimicrobial activity and compound 1 does not, demonstrates that the exocyclic conjugated methylene in the lactone ring is essential for the antimicrobial activity of these sesquiterpene lactones. However, the weak activity observed for the plant extracts, does not explain the use of Gynoxys verrucosa in traditional medicine for the treatment of wounds and skin infections. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  18. Automating gene library synthesis by structure-based combinatorial protein engineering: examples from plant sesquiterpene synthases.

    Science.gov (United States)

    Dokarry, Melissa; Laurendon, Caroline; O'Maille, Paul E

    2012-01-01

    Structure-based combinatorial protein engineering (SCOPE) is a homology-independent recombination method to create multiple crossover gene libraries by assembling defined combinations of structural elements ranging from single mutations to domains of protein structure. SCOPE was originally inspired by DNA shuffling, which mimics recombination during meiosis, where mutations from parental genes are "shuffled" to create novel combinations in the resulting progeny. DNA shuffling utilizes sequence identity between parental genes to mediate template-switching events (the annealing and extension of one parental gene fragment on another) in PCR reassembly reactions to generate crossovers and hence recombination between parental genes. In light of the conservation of protein structure and degeneracy of sequence, SCOPE was developed to enable the "shuffling" of distantly related genes with no requirement for sequence identity. The central principle involves the use of oligonucleotides to encode for crossover regions to choreograph template-switching events during PCR assembly of gene fragments to create chimeric genes. This approach was initially developed to create libraries of hybrid DNA polymerases from distantly related parents, and later developed to create a combinatorial mutant library of sesquiterpene synthases to explore the catalytic landscapes underlying the functional divergence of related enzymes. This chapter presents a simplified protocol of SCOPE that can be integrated with different mutagenesis techniques and is suitable for automation by liquid-handling robots. Two examples are presented to illustrate the application of SCOPE to create gene libraries using plant sesquiterpene synthases as the model system. In the first example, we outline how to create an active-site library as a series of complex mixtures of diverse mutants. In the second example, we outline how to create a focused library as an array of individual clones to distil minimal combinations of

  19. Anti-Trypanosomatid Elemanolide Sesquiterpene Lactones from Vernonia lasiopus O. Hoffm.

    Science.gov (United States)

    Kimani, Njogu M; Matasyoh, Josphat C; Kaiser, Marcel; Brun, Reto; Schmidt, Thomas J

    2017-04-08

    Sleeping sickness or human African trypanosomiasis (HAT) is a neglected tropical disease (NTD) threatening millions of peoples' lives with thousands infected. The disease is endemic in poorly developed regions of sub-Saharan Africa and is caused by the kinetoplastid "protozoan" parasite Trypanosoma brucei . The parasites are transmitted to humans through bites of infected tsetse flies of the genus Glossina . The few available drugs for treatment of this disease are highly toxic, difficult to administer, costly and unavailable to poor rural communities bearing the major burden of this infection. Therefore, the search for new efficacious, safe and affordable drugs is of high importance. Vernonia lasiopus O. Hoffm., an indigenous African plant of the Asteraceae family, has been extensively reported to be used ethno-medicinally as a treatment for malaria. Its crude extracts obtained with solvents of different polarity were screened in vitro for anti-protozoal activity and the dichloromethane extract was found to be particularly active against Trypanosoma brucei rhodesiense (IC 50 = 0.17 µg/mL). Bioassay-guided chromatographic fractionation of the dichloromethane extract led to the isolation and identification of six elemanolide type sesquiterpene lactones: 8-desacylvernolide, vernolepin, vernomenin, vernodalol, vernodalin and 11,13-dihydrovernodalin. All these elemanolide sesquiterpene lactones showed in vitro anti-trypanosomal activity. They were also tested for cytotoxicity against mammalian cells (L6 cell line). Vernolepin, the main component in the extract, was also the most potent with an IC 50 value of 0.05 µg/mL against T.b. rhodesiense trypomastigotes. This compound showed a selectivity index of 14.5, which makes it an interesting candidate for in vivo tests and determination of its mechanism of action.

  20. Potent immunosuppressive principles, dimeric sesquiterpene thioalkaloids, isolated from nupharis rhizoma, the rhizoma of Nuphar pumilum (nymphaeaceae): structure-requirement of nuphar-alkaloid for immunosuppressive activity.

    Science.gov (United States)

    Yamahara, J; Shimoda, H; Matsuda, H; Yoshikawa, M

    1996-09-01

    Potent immunosuppressants, the dimeric sesquiterpene thioalkaloids, 6-hydroxythiobinupharidine (2), 6,6'-dihydroxythiobinupharidine (3), 6-hydroxythionuphlutine B (5) and 6'-hydroxythionuphlutine B (6), were isolated from a natural medicine, Nupharis Rhizoma, the rhizoma of Nuphar pumilum (TIMM.) DC., through bioassay-guided separation together with five quinolizidine alkaloids (8, 9, 10, 11, 12). Dimeric sesquiterpene thioalkaloids (2, 3, 5, 6) were found to significantly inhibit anti-sheep erythrocyte plaque forming cell formation in mice spleen cells at 10(-6) M concentration. At this concentration, 2, 5 and 6 were found to exhibit no cytotoxicity to mice spleen cells, and 3 also showed only a little cytotoxicity. In addition, the inhibitory activity of several Nuphar alkaloids, dimeric sesquiterpene thioalkaloids (1, 4, 7, 8), and monomeric sesquiterpene alkaloids (9, 10, 11, 12) on anti-sheep erythrocyte plaque forming cell formation was examined and some structural requirement of Nuphar alkaloid for immunosuppressive activity was determined.

  1. Metabolite profiling of sesquiterpene lactones from Lactuca species. Major latex components are novel oxalate and sulfate conjugates of lactucin and its derivatives.

    Science.gov (United States)

    Sessa, R A; Bennett, M H; Lewis, M J; Mansfield, J W; Beale, M H

    2000-09-01

    Wounding leaves or stems of Lactuca species releases a milky latex onto the plant surface. We have examined the constituents of latex from Lactuca sativa (lettuce) cv. Diana. The major components were shown to be novel 15-oxalyl and 8-sulfate conjugates of the guaianolide sesquiterpene lactones, lactucin, deoxylactucin, and lactucopicrin. The oxalates were unstable, reverting to the parent sesquiterpene lactone on hydrolysis. Oxalyl derivatives have been reported rarely from natural sources. The sulfates were stable and are the first reported sesquiterpene sulfates from plants. Unusual tannins based on 4-hydroxyphenylacetyl conjugates of glucose were also identified. Significant qualitative and quantitative variation was found in sesquiterpene lactone profiles in different lettuce varieties and in other Lactuca spp. The proportions of each conjugate in latex also changed depending on the stage of plant development. A similar profile was found in chicory, in which oxalyl conjugates were identified, but the 8-sulfate conjugates were notably absent. The presence of the constitutive sesquiterpene lactones was not correlated with resistance to pathogens but may have a significant bearing on the molecular basis of the bitter taste of lettuce and related species. The induced sesquiterpene lactone phytoalexin, lettucenin A, was found in the Lactuca spp. but not in chicory.

  2. Antiproliferative activity against leukemia cells of sesquiterpene lactones from the Turkish endemic plant Centaurea drabifolia subsp. detonsa.

    Science.gov (United States)

    Formisano, Carmen; Sirignano, Carmina; Rigano, Daniela; Chianese, Giuseppina; Zengin, Gokhan; Seo, Ean-Jeong; Efferth, Thomas; Taglialatela-Scafati, Orazio

    2017-07-01

    The apolar organic extract obtained from aerial parts of Centaurea drabifolia Sibth. & Sm. subsp. detonsa (Bornm.) Wagenitz, growing wild in Turkey, was investigated for the first time for its secondary metabolite composition. Seven sesquiterpene lactones belonging to the guaiane class (1-7), including the new compound 4, along with a fatty acid lactone derivative (8), were isolated. The structures of these compounds were established by spectroscopic analysis, including 2D NMR spectroscopic techniques, with the stereostructure of the new guaiane 4 determined with the help of MTPA derivatization. Cytotoxic activities of compounds 1-7 were evaluated against two cancer cell lines, namely acute lymphoblastic leukemia (CCRF-CEM) and its multidrug-resistant subline CEM/ADR5000. Results showed that aguerin B (1) and cynaropicrin (2) showed a potent activity on both cell lines revealing interesting details about the structure-activity relationships in the class of acylated guaiane sesquiterpenes. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. The role of germacrene D as a precursor in sesquiterpene biosynthesis: investigations of acid catalyzed, photochemically and thermally induced rearrangements.

    Science.gov (United States)

    Bülow, N; Konig, W A

    2000-09-01

    Germacrene D is considered as a precursor of many sesquiterpene hydrocarbons. We have investigated the acid catalyzed as well as the photochemically and thermally induced rearrangement processes of germacrene D isolated from several Solidago species, which contain both enantiomers of germacrene D. Enantiomeric mixtures of sesquiterpenes of the cadinane, eudesmane (selinane), oppositane, axane, isodaucane, and bourbonane group as well as isogermacrene D were identified as main products and made available as reference compounds for structure investigations and stereochemical assignments of plant constituents. Delta-amorphene, one of the rearrangement products, was identified as a natural product for the first time. The absolute configuration of gamma-amorphene was revised by correlation with the absolute configuration of germacrene D. The mechanisms of the rearrangement reactions are discussed.

  4. Two terpene synthases are responsible for the major sesquiterpenes emitted from the flowers of kiwifruit (Actinidia deliciosa)

    Science.gov (United States)

    Nieuwenhuizen, Niels J.; Wang, Mindy Y.; Matich, Adam J.; Green, Sol A.; Chen, Xiuyin; Yauk, Yar-Khing; Beuning, Lesley L.; Nagegowda, Dinesh A.; Dudareva, Natalia; Atkinson, Ross G.

    2009-01-01

    Kiwifruit vines rely on bees for pollen transfer between spatially separated male and female individuals and require synchronized flowering to ensure pollination. Volatile terpene compounds, which are important cues for insect pollinator attraction, were studied by dynamic headspace sampling in the major green-fleshed kiwifruit (Actinidia deliciosa) cultivar ‘Hayward’ and its male pollinator ‘Chieftain’. Terpene volatile levels showed a profile dominated by the sesquiterpenes α-farnesene and germacrene D. These two compounds were emitted by all floral tissues and could be observed throughout the day, with lower levels at night. The monoterpene (E)-β-ocimene was also detected in flowers but was emitted predominantly during the day and only from petal tissue. Using a functional genomics approach, two terpene synthase (TPS) genes were isolated from a ‘Hayward’ petal EST library. Bacterial expression and transient in planta data combined with analysis by enantioselective gas chromatography revealed that one TPS produced primarily (E,E)-α-farnesene and small amounts of (E)-β-ocimene, whereas the second TPS produced primarily (+)-germacrene D. Subcellular localization using GFP fusions showed that both enzymes were localized in the cytoplasm, the site for sesquiterpene production. Real-time PCR analysis revealed that both TPS genes were expressed in the same tissues and at the same times as the corresponding floral volatiles. The results indicate that two genes can account for the major floral sesquiterpene volatiles observed in both male and female A. deliciosa flowers. PMID:19516075

  5. Dihydro-β-agarofuran sesquiterpenes from celastraceae species as anti-tumour-promoting agents: Structure-activity relationship.

    Science.gov (United States)

    Núñez, Marvin J; Jiménez, Ignacio A; Mendoza, Cristina R; Chavez-Sifontes, Marvin; Martinez, Morena L; Ichiishi, Eiichiro; Tokuda, Ryo; Tokuda, Harukuni; Bazzocchi, Isabel L

    2016-03-23

    Inhibition of tumour promotion in multistage chemical carcinogenesis is considered a promising strategy for cancer chemoprevention. In an ongoing investigation of bioactive secondary metabolites from Celastraceae species, five new dihydro-β-agarofuran sesquiterpenes (1-5), named Chiapens A-E, and seventeen known ones, were isolated from Maytenus chiapensis. Their structures were elucidated by extensive NMR spectroscopic and mass spectrometric techniques, and their absolute configurations were determined by circular dichroism studies, chemical correlations and biogenic means. The isolated compounds, along with twenty known sesquiterpenes, previously isolated from Zinowiewia costaricensis, have been tested for their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation induced by 12-O-tetradecanoylphorpol-13-acetate (TPA). Thirty three compounds from this series showed stronger effects than that of β-carotene, the reference inhibitor. The structure-activity relationship (SAR) analysis revealed that the type of substituent, in particular at the C-1 position of the sesquiterpene scaffold, was able to modulate the anti-tumour promoting activity. Compounds 3, 6, and 33 showed significant effects in an in vivo two-stage mouse-skin carcinogenesis model. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  6. Tuberculose infantil: estudo retrospectivo

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    Boaventura Antonio dos Santos

    2011-10-01

    Full Text Available Introdução: A tuberculose (TB infantil permanece como uma das doenças mais prevalentes e preocupantes no mundo, sobretudo em nações em desenvolvimento, onde as taxas são ainda mais elevadas e os casos descritos subestimados pela dificuldade em se estabelecer um diagnóstico definitivo. Dessa forma, este estudo tem como objetivo descrever o perfil clínico e epidemiológico dos pacientes com TB infantil pulmonar e extrapulmonar. Métodos: Foram avaliados retrospectivamente, através de questionário, pacientes com idade de até 15 anos, internados no Serviço de Pediatria do HCPA, no período de janeiro de 2002 a setembro de 2007.   Resultados: Dos 52 pacientes incluídos, 63% apresentavam TB pulmonar. Das formas extra-pulmonares, a meningoencefalite foi a mais prevalente (22%. Comorbidades foram dectadas em 31 (60% pacientes, dos quais 15 (29% apresentavam desnutrição grave, 9 (18% HIV positivo e 7 (13% pneumopatia crônica. Das manifestações clínicas, febre e tosse estavam presentes na maioria dos pacientes. O padrão radiológico predominante foi o de consolidação pulmonar (51%. A maioria dos pacientes referia história de contato com paciente bacilífero (64%. Conclusão: A TB pulmonar representa a principal forma de apresentação clínica da TB, sendo o diagnóstico feito de forma presuntiva na maioria dos casos. O diagnóstico baseado na comprovação bacteriológica foi obtido numa minoria de pacientes, demonstrando a importância dos achados clínico-laboratoriais, história epidemiológica e vacinal para o diagnóstico. Nesse sentido, a criação de escores tem se tornado uma ferramenta de fácil acesso e com razoável acurácia para auxiliar o diagnóstico de TB em serviços de baixa complexidade, especialmente o ambulatorial.

  7. In-situ ambient quantification of monoterpenes, sesquiterpenes, and related oxygenated compounds during BEARPEX 2007: implications for gas- and particle-phase chemistry

    Directory of Open Access Journals (Sweden)

    N. C. Bouvier-Brown

    2009-08-01

    Full Text Available We quantified ambient mixing ratios of 9 monoterpenes, 6 sesquiterpenes, methyl chavicol, the oxygenated terpene linalool, and nopinone using an in-situ gas chromatograph with a quadrupole mass spectrometer (GC-MS. These measurements were a part of the 2007 Biosphere Effects on AeRosols and Photochemistry EXperiment (BEARPEX at Blodgett Forest, a ponderosa pine forest in the Sierra Nevada Mountains of California. To our knowledge, these observations represent the first direct in-situ ambient quantification of the sesquiterpenes α-bergamotene, longifolene, α-farnesene, and β-farnesene. From average diurnal mixing ratio profiles, we show that α-farnesene emissions are dependent mainly on temperature whereas α-bergamotene and β-farnesene emissions are temperature- and light-dependent. The amount of sesquiterpene mass quantified above the canopy was small (averaging a total of 3.3 ppt during the day, but nevertheless these compounds contributed 7.6% to the overall ozone-olefin loss rate above the canopy. Assuming that the monoterpene-to-sesquiterpene emission rate in the canopy is similar to that observed in branch enclosure studies at the site during comparable weather conditions, and the average yield of aerosol mass from these sesquiterpenes is 10–50%, the amount of sesquiterpene mass reacted within the Blodgett Forest canopy alone accounts for 6–32% of the total organic aerosol mass measured during BEARPEX. The oxygenated monoterpene linalool was also quantified for the first time at Blodgett Forest. The linalool mass contribution was small (9.9 ppt and 0.74 ppt within and above the canopy, respectively, but it contributed 1.1% to the total ozone-olefin loss rate above the canopy. Reactive and semi-volatile compounds, especially sesquiterpenes, significantly impact the gas- and particle-phase chemistry of the atmosphere at Blodgett Forest and should be included in both biogenic volatile organic carbon emission and atmospheric chemistry

  8. In-situ ambient quantification of monoterpenes, sesquiterpenes, and related oxygenated compounds during BEARPEX 2007: implications for gas- and particle-phase chemistry

    Science.gov (United States)

    Bouvier-Brown, N. C.; Goldstein, A. H.; Gilman, J. B.; Kuster, W. C.; de Gouw, J. A.

    2009-08-01

    We quantified ambient mixing ratios of 9 monoterpenes, 6 sesquiterpenes, methyl chavicol, the oxygenated terpene linalool, and nopinone using an in-situ gas chromatograph with a quadrupole mass spectrometer (GC-MS). These measurements were a part of the 2007 Biosphere Effects on AeRosols and Photochemistry EXperiment (BEARPEX) at Blodgett Forest, a ponderosa pine forest in the Sierra Nevada Mountains of California. To our knowledge, these observations represent the first direct in-situ ambient quantification of the sesquiterpenes α-bergamotene, longifolene, α-farnesene, and β-farnesene. From average diurnal mixing ratio profiles, we show that α-farnesene emissions are dependent mainly on temperature whereas α-bergamotene and β-farnesene emissions are temperature- and light-dependent. The amount of sesquiterpene mass quantified above the canopy was small (averaging a total of 3.3 ppt during the day), but nevertheless these compounds contributed 7.6% to the overall ozone-olefin loss rate above the canopy. Assuming that the monoterpene-to-sesquiterpene emission rate in the canopy is similar to that observed in branch enclosure studies at the site during comparable weather conditions, and the average yield of aerosol mass from these sesquiterpenes is 10-50%, the amount of sesquiterpene mass reacted within the Blodgett Forest canopy alone accounts for 6-32% of the total organic aerosol mass measured during BEARPEX. The oxygenated monoterpene linalool was also quantified for the first time at Blodgett Forest. The linalool mass contribution was small (9.9 ppt and 0.74 ppt within and above the canopy, respectively), but it contributed 1.1% to the total ozone-olefin loss rate above the canopy. Reactive and semi-volatile compounds, especially sesquiterpenes, significantly impact the gas- and particle-phase chemistry of the atmosphere at Blodgett Forest and should be included in both biogenic volatile organic carbon emission and atmospheric chemistry models.

  9. In-situ ambient quantification of monoterpenes, sesquiterpenes, and related oxygenated compounds during BEARPEX 2007 - implications for gas- and particle-phase chemistry

    Science.gov (United States)

    Bouvier-Brown, N. C.; Goldstein, A. H.; Gilman, J. B.; Kuster, W. C.; de Gouw, J. A.

    2009-04-01

    We quantified ambient mixing ratios of 9 monoterpenes, 6 sesquiterpenes, methyl chavicol, the oxygenated terpene linalool, and nopinone using an in-situ gas chromatograph with a quadrupole mass spectrometer (GC-MS). These measurements were a part of the 2007 Biosphere Effects on AeRosols and Photochemistry EXperiment (BEARPEX) at Blodgett Forest, a ponderosa pine forest in the Sierra Nevada Mountains of California. To our knowledge, these observations represent the first direct in-situ ambient quantification of the sesquiterpenes α-bergamotene, longifolene, α-farnesene, and β-farnesene. From average diurnal mixing ratio profiles, we show that α-farnesene emissions are dependent mainly on temperature whereas α-bergamotene and β-farnesene emissions are temperature- and light-dependent. The amount of sesquiterpene mass quantified above the canopy was small (averaging a total of 3.3 ppt during the day), but nevertheless these compounds contributed 8.5% to the overall ozone reactivity above the canopy. Assuming that the monoterpene-to-sesquiterpene emission rate in the canopy is similar to that observed in branch enclosure studies at the site during comparable weather conditions, and the average yield of aerosol mass from these sesquiterpenes is 10-50%, the amount of sesquiterpene mass reacted within the Blodgett Forest canopy alone accounts for 8-38% of the total organic aerosol mass measured during BEARPEX. The oxygenated monoterpene linalool was also quantified for the first time at Blodgett Forest. The linalool mass contribution was small (9.9 ppt and 0.74 ppt within and above the canopy, respectively), but it contributed 1.2% to the total ozone reactivity above the canopy. Reactive and semi-volatile compounds, especially sesquiterpenes, significantly impact the gas- and particle-phase chemistry of the atmosphere at Blodgett Forest and should be included in both biogenic volatile organic carbon emission and atmospheric chemistry models.

  10. Celastraceae sesquiterpenes as a new class of modulators that bind specifically to human P-glycoprotein and reverse cellular multidrug resistance.

    Science.gov (United States)

    Muñoz-Martínez, Francisco; Lu, Peihua; Cortés-Selva, Fernando; Pérez-Victoria, José María; Jiménez, Ignacio A; Ravelo, Angel G; Sharom, Frances J; Gamarro, Francisco; Castanys, Santiago

    2004-10-01

    Overexpression of ABCB1 (MDR1) P-glycoprotein, a multidrug efflux pump, is one mechanism by which tumor cells may develop multidrug resistance (MDR), preventing the successful chemotherapeutic treatment of cancer. Sesquiterpenes from Celastraceae family are natural compounds shown previously to reverse MDR in several human cancer cell lines and Leishmania strains. However, their molecular mechanism of reversion has not been characterized. In the present work, we have studied the ability of 28 dihydro-beta-agarofuran sesquiterpenes to reverse the P-glycoprotein-dependent MDR phenotype and elucidated their molecular mechanism of action. Cytotoxicity assays using human MDR1-transfected NIH-3T3 cells allowed us to select the most potent sesquiterpenes reversing the in vitro resistance to daunomycin and vinblastine. Flow cytometry experiments showed that the above active compounds specifically inhibited drug transport activity of P-glycoprotein in a saturable, concentration-dependent manner (K(i) down to 0.24 +/- 0.01 micromol/L) but not that of ABCC1 (multidrug resistance protein 1; MRP1), ABCC2 (MRP2), and ABCG2 (breast cancer resistance protein; BCRP) transporters. Moreover, sesquiterpenes inhibited at submicromolar concentrations the P-glycoprotein-mediated transport of [(3)H]colchicine and tetramethylrosamine in plasma membrane from CH(R)B30 cells and P-glycoprotein-enriched proteoliposomes, supporting that P-glycoprotein is their molecular target. Photoaffinity labeling in plasma membrane and fluorescence spectroscopy experiments with purified protein suggested that sesquiterpenes interact with transmembrane domains of P-glycoprotein. Finally, sesquiterpenes modulated P-glycoprotein ATPase-activity in a biphasic, concentration-dependent manner: they stimulated at very low concentrations but inhibited ATPase activity as noncompetitive inhibitors at higher concentrations. Sesquiterpenes from Celastraceae are promising P-glycoprotein modulators with potential

  11. Cholinesterase Inhibition and Molecular Docking Studies of Sesquiterpene Coumarin Ethers from Heptaptera cilicica

    Directory of Open Access Journals (Sweden)

    Zühal Güvenalp

    2017-09-01

    Full Text Available Five sesquiterpene coumarin ethers: umbelliprenin, umbelliprenin-10',11'-monoepoxide, conferone, mogoltacin and feselol were isolated from the fruits of Heptaptera cilicica. Their structures were identified by means of spectroscopic methods. AChE and BuChE inhibitory activities of the compounds were determined by molecular docking method which were confirmed by in vitro experiments. According to molecular docking results, total score of feselol and umbelliprenin were 5.69 and 3.23 kcal/mol against acetylcholinesterase, respectively. Total score for butyrylcholinesterase inhibitory effect of them were 2.76 and 4.99 kcal/mol, respectively. Feselol and umbelliprenin exhibited significantly high inhibitory potency against acetylcholinesterase (IC 50 = 1.26 ± 0.01 and 5.86 ± 0.03 μM, respectively and butyrylcholinesterase (IC 50 = 9.98 ± 0.24 and 1.10 ± 0.19 μM, respectively. This is the first report of isolation of natural bioactives obtained from the chloroform extract of Heptaptera cilicica fruits with anticholinesterase activity.

  12. Antiprotozoal and Antiglycation Activities of Sesquiterpene Coumarins from Ferula narthex Exudate

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    Adnan Amin

    2016-09-01

    Full Text Available The exudate of Ferula narthex Boiss. (Apiaceae is widely used in the Indian subcontinent as a spice and because of its health effects. Six sesquiterpene coumarins have been isolated from this exudate: feselol, ligupersin A, asacoumarin A, 8′-O-acetyl-asacoumarin A, 10′R-karatavacinol and 10′R-acetyl-karatavacinol. Based on its use in infectious and diabetic conditions, the isolated constituents were evaluated for antimicrobial and antiglycation activities. Some compounds showed activity against protozoal parasites, asacoumarin A being the most active one against Plasmodium falciparum K1 (IC50 1.3 μM. With regard to antiglycation activity, in the BSA-glucose test, ligupersin A displayed the highest activity (IC50 0.41 mM, being more active than the positive control aminiguanidine (IC50 1.75 mM. In the BSA-MGO assay, the highest activity was shown by 8′-O-acetyl-asacoumarin A (IC50 1.03 mM, being less active than aminoguanidine (IC50 0.15 mM. Hence, the antiglycation activity of the isolated constituents was due to both oxidative and non-oxidative modes of inhibition.

  13. Sesquiterpene lactones induce distinct forms of cell death that modulate human monocyte-derived macrophage responses.

    Science.gov (United States)

    López-Antón, Nancy; Hermann, Corinna; Murillo, Renato; Merfort, Irmgard; Wanner, Gerhard; Vollmar, Angelika M; Dirsch, Verena M

    2007-01-01

    Sesquiterpene lactones (SQTLs) are shown to possess anti-inflammatory as well as cytotoxic activity. No study, however, links both activities. We, therefore, hypothesized that SQTL-treated, dying cells might induce an anti-inflammatory response in cocultured THP-1 macrophages. Here we show that SQTLs bearing either an alpha,beta-unsaturated cyclopentenone or an alpha-methylene-gamma-lactone induce different forms of cell death. Whereas the cyclopentenone SQTL induced typical apoptosis, the alpha-methylene-gamma-lactone SQTLs-induced cell death lacked partly classical signs of apoptosis, such as DNA fragmentation. All SQTLs, however, activated caspases and the nuclear morphology of cell death was dependent on caspase activation. Most interestingly, alpha-methylene-gamma-lactone SQTLs induced a more pronounced phosphatidylserine (PS) exposure than the cyclopentenone SQTL. Especially, 7-hydroxycostunolide (HC), with an alpha-methylene-gamma-lactone substituted with a hydroxyl group, showed a striking fast and pronounced PS translocation. This result was in agreement with a strong activation of phagocytosis in cocultured THP-1 macrophages. Interestingly, HC-treated Jurkat cells led to an early (3.5 h) but transient increase in TNF-alpha levels in macrophage coculture. Release of TGF-beta remained unaffected after 18 h. We propose that this type of SQTL may influence local inflammation by transiently activating the immune system and help to clear cells by inducing a form of cell death that promotes phagocytosis.

  14. Nerolidol: A Sesquiterpene Alcohol with Multi-Faceted Pharmacological and Biological Activities

    Directory of Open Access Journals (Sweden)

    Weng-Keong Chan

    2016-04-01

    Full Text Available Nerolidol (3,7,11-trimethyl-1,6,10-dodecatrien-3-ol is a naturally occurring sesquiterpene alcohol that is present in various plants with a floral odor. It is synthesized as an intermediate in the production of (3E-4,8-dimethy-1,3,7-nonatriene (DMNT, a herbivore-induced volatile that protects plants from herbivore damage. Chemically, nerolidol exists in two geometric isomers, a trans and a cis form. The usage of nerolidol is widespread across different industries. It has been widely used in cosmetics (e.g., shampoos and perfumes and in non-cosmetic products (e.g., detergents and cleansers. In fact, U.S. Food and Drug Administration (FDA has also permitted the use of nerolidol as a food flavoring agent. The fact that nerolidol is a common ingredient in many products has attracted researchers to explore more medicinal properties of nerolidol that may exert beneficial effect on human health. Therefore, the aim of this review is to compile and consolidate the data on the various pharmacological and biological activities displayed by nerolidol. Furthermore, this review also includes pharmacokinetic and toxicological studies of nerolidol. In summary, the various pharmacological and biological activities demonstrated in this review highlight the prospects of nerolidol as a promising chemical or drug candidate in the field of agriculture and medicine.

  15. Role of needle surface waxes in dynamic exchange of mono- and sesquiterpenes

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    J. Joensuu

    2016-06-01

    Full Text Available Biogenic volatile organic compounds (BVOCs produced by plants have a major role in atmospheric chemistry. The different physicochemical properties of BVOCs affect their transport within and out of the plant as well as their reactions along the way. Some of these compounds may accumulate in or on the waxy surface layer of conifer needles and participate in chemical reactions on or near the foliage surface. The aim of this work was to determine whether terpenes, a key category of BVOCs produced by trees, can be found on the epicuticles of Scots pine (Pinus sylvestris L. and, if so, how they compare with the terpenes found in shoot emissions of the same tree. We measured shoot-level emissions of pine seedlings at a remote outdoor location in central Finland and subsequently analysed the needle surface waxes for the same compounds. Both emissions and wax extracts were clearly dominated by monoterpenes, but the proportion of sesquiterpenes was higher in the wax extracts. There were also differences in the terpene spectra of the emissions and the wax extracts. The results, therefore, support the existence of BVOC associated to the epicuticular waxes. We briefly discuss the different pathways for terpenes to reach the needle surfaces and the implications for air chemistry.

  16. Biotransformation of the sesquiterpene (+)-valencene by cytochrome P450cam and P450BM-3.

    Science.gov (United States)

    Sowden, Rebecca J; Yasmin, Samina; Rees, Nicholas H; Bell, Stephen G; Wong, Luet-Lok

    2005-01-07

    The sesquiterpenoids are a large class of naturally occurring compounds with biological functions and desirable properties. Oxidation of the sesquiterpene (+)-valencene by wild type and mutants of P450cam from Pseudomonas putida, and of P450BM-3 from Bacillus megaterium, have been investigated as a potential route to (+)-nootkatone, a fine fragrance. Wild type P450cam did not oxidise (+)-valencene but the mutants showed activities up to 9.8 nmol (nmol P450)(-1) min(-1), with (+)-trans-nootkatol and (+)-nootkatone constituting >85% of the products. Wild type P450BM-3 and mutants had higher activities (up to 43 min(-1)) than P450cam but were much less selective. Of the many products, cis- and trans-(+)-nootkatol, (+)-nootkatone, cis-(+)-valencene-1,10-epoxide, trans-(+)-nootkaton-9-ol, and (+)-nootkatone-13S,14-epoxide were isolated from whole-cell reactions and characterised. The selectivity patterns suggest that (+)-valencene has one binding orientation in P450cam but multiple orientations in P450BM-3.

  17. Guaiane sesquiterpenes from seaweed Ulva fasciata Delile and their antibacterial properties.

    Science.gov (United States)

    Chakraborty, Kajal; Lipton, A P; Paulraj, R; Chakraborty, Rekha D

    2010-06-01

    Two new guaiane sesquiterpene derivatives, guai-2-en-10alpha-ol (1) and guai-2-en-10alpha-methanol (2), were chromatographically purified as major constituents of the CHCl3/CH3OH (1:1, v/v) soluble fraction of Ulva fasciata. Acetylation of 2 furnished guai-2-en-10alpha-methyl methanoate (3) with acetyl group at C11 position. The structures of the compounds were elucidated using one and two-dimensional NMR and mass spectrometric analysis. Compounds 2 and 3 exhibited significant inhibition to the growth of Vibrio parahaemolyticus with minimum inhibitory concentrations of 25 and 35 microg/mL, respectively. The electronegative C10 acetyl group with high polarisability (7.02x10(-24) cm3) in 3 appeared to withdraw electron cloud from substituted cycloheptyl ring and (R)-3-methylcyclohept-1-ene moiety, thus acting as the nucleophilic center of the molecule resulting in high bioactivity. Copyright (c) 2010 Elsevier Masson SAS. All rights reserved.

  18. Sesquiterpene lactones: Mechanism of antineoplastic activity; relationship of cellular glutathione to cytotoxicity; and disposition

    International Nuclear Information System (INIS)

    Grippo, A.A.

    1987-01-01

    Helenalin, a sesquiterpene lactone, inhibited the growth of P388 lymphocytic and L1210 lymphoid leukemia, and Ehrlich ascites and KB carcinoma cells. The L1210 leukemia cells were most sensitive to the cytotoxic effects of helenalin. Helenalin's antineoplastic effects were due to inhibition of DNA synthesis by suppressing the activities of enzymes involved in this biosynthetic pathway; i.e., IMP dehydrogenase, ribonucleoside diphosphate reductase, thioredoxin complex, GSH disulfide oxidoreductase and DNA polymerase α activities. The relationship of reduced glutathione (GSH) to the cytotoxic effects of helanalin was evaluated. L1210 cells, which were more sensitive to helenalin's toxicity, contained lower basal concentrations of GSH. Helenalin decreased the concentration of reduced glutathione in both L1210 and P388 leukemia cells. Concurrent administration of helanalin with agents reported to raise GSH concentrations did not substantially effect GSH levels, nor were survival times of tumor-bearing mice enhanced. Following intraperitoneal administration of 3 H-plenolin, no radioactive drug and/or metabolite was sequestered in the organs of BDF 1 mice. Approximately 50% of 3 H-plenolin and/or its metabolites were eliminated via urine while lesser amounts of radioactive drug and/or metabolites were eliminated in the feces

  19. Functional identification of valerena-1,10-diene synthase, a terpene synthase catalyzing a unique chemical cascade in the biosynthesis of biologically active sesquiterpenes in Valeriana officinalis.

    Science.gov (United States)

    Yeo, Yun-Soo; Nybo, S Eric; Chittiboyina, Amar G; Weerasooriya, Aruna D; Wang, Yan-Hong; Góngora-Castillo, Elsa; Vaillancourt, Brieanne; Buell, C Robin; DellaPenna, Dean; Celiz, Mary Dawn; Jones, A Daniel; Wurtele, Eve Syrkin; Ransom, Nick; Dudareva, Natalia; Shaaban, Khaled A; Tibrewal, Nidhi; Chandra, Suman; Smillie, Troy; Khan, Ikhlas A; Coates, Robert M; Watt, David S; Chappell, Joe

    2013-02-01

    Valerian is an herbal preparation from the roots of Valeriana officinalis used as an anxiolytic and sedative and in the treatment of insomnia. The biological activities of valerian are attributed to valerenic acid and its putative biosynthetic precursor valerenadiene, sesquiterpenes, found in V. officinalis roots. These sesquiterpenes retain an isobutenyl side chain whose origin has been long recognized as enigmatic because a chemical rationalization for their biosynthesis has not been obvious. Using recently developed metabolomic and transcriptomic resources, we identified seven V. officinalis terpene synthase genes (VoTPSs), two that were functionally characterized as monoterpene synthases and three that preferred farnesyl diphosphate, the substrate for sesquiterpene synthases. The reaction products for two of the sesquiterpene synthases exhibiting root-specific expression were characterized by a combination of GC-MS and NMR in comparison to the terpenes accumulating in planta. VoTPS7 encodes for a synthase that biosynthesizes predominately germacrene C, whereas VoTPS1 catalyzes the conversion of farnesyl diphosphate to valerena-1,10-diene. Using a yeast expression system, specific labeled [(13)C]acetate, and NMR, we investigated the catalytic mechanism for VoTPS1 and provide evidence for the involvement of a caryophyllenyl carbocation, a cyclobutyl intermediate, in the biosynthesis of valerena-1,10-diene. We suggest a similar mechanism for the biosynthesis of several other biologically related isobutenyl-containing sesquiterpenes.

  20. Functional Identification of Valerena-1,10-diene Synthase, a Terpene Synthase Catalyzing a Unique Chemical Cascade in the Biosynthesis of Biologically Active Sesquiterpenes in Valeriana officinalis*

    Science.gov (United States)

    Yeo, Yun-Soo; Nybo, S. Eric; Chittiboyina, Amar G.; Weerasooriya, Aruna D.; Wang, Yan-Hong; Góngora-Castillo, Elsa; Vaillancourt, Brieanne; Buell, C. Robin; DellaPenna, Dean; Celiz, Mary Dawn; Jones, A. Daniel; Wurtele, Eve Syrkin; Ransom, Nick; Dudareva, Natalia; Shaaban, Khaled A.; Tibrewal, Nidhi; Chandra, Suman; Smillie, Troy; Khan, Ikhlas A.; Coates, Robert M.; Watt, David S.; Chappell, Joe

    2013-01-01

    Valerian is an herbal preparation from the roots of Valeriana officinalis used as an anxiolytic and sedative and in the treatment of insomnia. The biological activities of valerian are attributed to valerenic acid and its putative biosynthetic precursor valerenadiene, sesquiterpenes, found in V. officinalis roots. These sesquiterpenes retain an isobutenyl side chain whose origin has been long recognized as enigmatic because a chemical rationalization for their biosynthesis has not been obvious. Using recently developed metabolomic and transcriptomic resources, we identified seven V. officinalis terpene synthase genes (VoTPSs), two that were functionally characterized as monoterpene synthases and three that preferred farnesyl diphosphate, the substrate for sesquiterpene synthases. The reaction products for two of the sesquiterpene synthases exhibiting root-specific expression were characterized by a combination of GC-MS and NMR in comparison to the terpenes accumulating in planta. VoTPS7 encodes for a synthase that biosynthesizes predominately germacrene C, whereas VoTPS1 catalyzes the conversion of farnesyl diphosphate to valerena-1,10-diene. Using a yeast expression system, specific labeled [13C]acetate, and NMR, we investigated the catalytic mechanism for VoTPS1 and provide evidence for the involvement of a caryophyllenyl carbocation, a cyclobutyl intermediate, in the biosynthesis of valerena-1,10-diene. We suggest a similar mechanism for the biosynthesis of several other biologically related isobutenyl-containing sesquiterpenes. PMID:23243312

  1. Biosynthesis of mono- and sesquiterpenes in strawberry fruits and foliage: 2H labeling studies.

    Science.gov (United States)

    Hampel, Daniela; Mosandl, Armin; Wüst, Matthias

    2006-02-22

    The biosynthesis of the monoterpene (S)-linalool and the sesquiterpene trans-(S)-nerolidol in fruits of Fragaria x ananassa Duch. cv. Eros and Florence and of the monoterpene (-)-alpha-pinene in Fragaria vesca was investigated by in vivo feeding experiments with [5,5-2H2]mevalonic acid lactone (d2-MVL) and [5,5-2H2]-1-deoxy-d-xylulose (d2-DOX). The feeding experiments indicate that (S)-linalool and trans-(S)-nerolidol in Fragaria x ananassa Duch. and (-)-alpha-pinene in F. vesca are exclusively synthesized via the cytosolic mevalonic acid pathway without any contribution from the plastidial 1-deoxy-D-xylulose/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) route. Inhibition experiments revealed that even the presence of mevastatin, an export of plastid-derived isopentyl diphosphate/dimethylallyl diphosphate, cannot be induced. However, the enantioselective analysis shows that in Fragaria x ananassa Duch. cv. Eros and Florence both linalool enantiomers are present and that only (S)-linalool is labeled after administration of d2-MVL. Therefore, the origin of (R)-linalool in these fruits remains unknown. Contrarily, in Fragaria x ananassa Duch. foliage (R)-linalool is the dominant enantiomer. Feeding experiments revealed an incorporation of d2-MVL and d2-DOX at equal rates exclusively into (S)-linalool. Only in F. vesca foliage, where (R)-linalool is present at high enantiomeric purity (ee > 90%), is a de novo biosynthesis of the (R)-enantiomer via the DOXP/MEP pathway detectable. These results demonstrate a complex intraplant variation of (R)- and (S)-linalool biosynthesis via the cytosolic and plastidial route.

  2. Assessment of sesquiterpene lactones isolated from Mikania plants species for their potential efficacy against Trypanosoma cruzi and Leishmania sp.

    Science.gov (United States)

    Laurella, Laura C; Cerny, Natacha; Bivona, Augusto E; Sánchez Alberti, Andrés; Giberti, Gustavo; Malchiodi, Emilio L; Martino, Virginia S; Catalan, Cesar A; Alonso, María Rosario; Cazorla, Silvia I; Sülsen, Valeria P

    2017-09-01

    Four sesquiterpene lactones, mikanolide, deoxymikanolide, dihydromikanolide and scandenolide, were isolated by a bioassay-guided fractionation of Mikania variifolia and Mikania micrantha dichloromethane extracts. Mikanolide and deoxymikanolide were the major compounds in both extracts (2.2% and 0.4% for Mikania variifolia and 21.0% and 6.4% for Mikania micrantha respectively, calculated on extract dry weight). Mikanolide, deoxymikanolide and dihydromikanolide were active against Trypanosoma cruzi epimastigotes (50% inhibitory concentrations of 0.7, 0.08 and 2.5 μg/mL, for each compound respectively). These sesquiterpene lactones were also active against the bloodstream trypomastigotes (50% inhibitory concentrations for each compound were 2.1, 1.5 and 0.3 μg/mL, respectively) and against amastigotes (50% inhibitory concentrations for each compound were 4.5, 6.3 and 8.5 μg/mL, respectively). By contrast, scandenolide was not active on Trypanosoma cruzi. Besides, mikanolide and deoxymikanolide were also active on Leishmania braziliensis promastigotes (50% inhibitory concentrations of 5.1 and 11.5 μg/mL, respectively). The four sesquiterpene lactones were tested for their cytotoxicity on THP 1 cells. Deoxymikanolide presented the highest selectivity index for trypomastigotes (SI = 54) and amastigotes (SI = 12.5). In an in vivo model of Trypanosoma cruzi infection, deoxymikanolide was able to decrease the parasitemia and the weight loss associated to the acute phase of the parasite infection. More importantly, while 100% of control mice died by day 22 after receiving a lethal T. cruzi infection, 70% of deoxymikanolide-treated mice survived. We also observed that this compound increased TNF-α and IL-12 production by macrophages, which could contribute to control T. cruzi infection.

  3. Irreversible impacts of heat on the emissions of monoterpenes, sesquiterpenes, phenolic BVOC and green leaf volatiles from several tree species

    Directory of Open Access Journals (Sweden)

    E. Kleist

    2012-12-01

    Full Text Available Climate change will induce extended heat waves to parts of the vegetation more frequently. High temperatures may act as stress (thermal stress on plants changing emissions of biogenic volatile organic compounds (BVOCs. As BVOCs impact the atmospheric oxidation cycle and aerosol formation, it is important to explore possible alterations of BVOC emissions under high temperature conditions. Applying heat to European beech, Palestine oak, Scots pine, and Norway spruce in a laboratory setup either caused the well-known exponential increases of BVOC emissions or induced irreversible changes of BVOC emissions. Considering only irreversible changes of BVOC emissions as stress impacts, we found that high temperatures decreased the de novo emissions of monoterpenes, sesquiterpenes and phenolic BVOC. This behaviour was independent of the tree species and whether the de novo emissions were constitutive or induced by biotic stress.

    In contrast, application of thermal stress to conifers amplified the release of monoterpenes stored in resin ducts of conifers and induced emissions of green leaf volatiles. In particular during insect attack on conifers, the plants showed de novo emissions of sesquiterpenes and phenolic BVOCs, which exceeded constitutive monoterpene emissions from pools. The heat-induced decrease of de novo emissions was larger than the increased monoterpene release caused by damage of resin ducts. For insect-infested conifers the net effect of thermal stress on BVOC emissions could be an overall decrease.

    Global change-induced heat waves may put hard thermal stress on plants. If so, we project that BVOC emissions increase is more than predicted by models only in areas predominantly covered with conifers that do not emit high amounts of sesquiterpenes and phenolic BVOCs. Otherwise overall effects of high temperature stress will be lower increases of BVOC emissions than predicted by algorithms that do

  4. Accumulation of the sesquiterpenes nootkatone and valencene by callus cultures of Citrus paradisi, Citrus limonia and Citrus aurantium.

    Science.gov (United States)

    Del Río, J A; Ortuño, A; Puig, D G; Iborra, J L; Sabater, F

    1991-10-01

    The production of the sesquiterpenes nootkatone and valencene by callus cultures of Citrus species is described. The levels of these compounds were examined by gas chromatography-mass spectrometry and their yields were compared with the amounts found in mature fruits. A simultaneous increase and decrease in the levels of nootkatone and valencene, respectively, were observed with the aging of callus cultures of Citrus paradisi. These results suggest that valencene might be a possible precursor of nootkatone in this species. The high level of nootkatone detected in 9-month-old callus cultures of Citrus paradisi might be associated with the corresponding cell morphological changes observed.

  5. Functional expression and characterization of sesquiterpene synthases from Artemisia annua L. using transient expression system in Nicotiana benthamiana.

    Science.gov (United States)

    Kanagarajan, Selvaraju; Muthusamy, Saraladevi; Gliszczyńska, Anna; Lundgren, Anneli; Brodelius, Peter E

    2012-07-01

    Artemisia annua L. produces a number of sesquiterpene synthases, which catalyze the conversion of farnesyl diphosphate to various sesquiterpenes. The cDNAs encoding amorpha-4,11-diene synthase (ADS), a key enzyme in the artemisinin biosynthesis, and epi-cedrol synthase (ECS), a complex sesquiterpene cyclization synthase, were cloned into Cowpea mosaic virus-based viral vector (pEAQ-HT) with Kozak consensus motif and C-terminal histidine tag. The plasmids were transformed into Agrobacterium LBA4404 and, agroinfiltrated into Nicotiana benthamiana leaves along with vector (pJL3:p19) containing Tomato bushy stunt virus post-transcriptional gene silencing suppressor. Quantitative PCR was carried out to measure the transcript levels at 0, 3, 6, 9, 12 and 15 days post-infiltration (dpi). The highest relative expression was observed at 9 dpi for both genes. Transiently expressed recombinant proteins of ADS and ECS were confirmed by SDS-PAGE and western blot. Recombinant proteins were extracted from 9 dpi leaves and purified by immobilized metal ion affinity chromatography using histidine tag, which produced yields of 90 and 96 mg kg⁻¹ fresh weight of leaves for ADS and ECS, respectively. Activities of the purified enzymes were assayed using gas chromatography-mass spectrometry for product identification and quantification using valencene as internal standard. The recombinant ADS and ECS converted farnesyl diphosphate into amorpha-4,11-diene (97 %) and epi-cedrol (96 %) as the major products, respectively. The purified enzymes exhibited the specific activity of 0.002 and 0.01 μmol min⁻¹ mg⁻¹ protein for ADS and ECS, respectively. The apparent k(cat) values were 2.1 × 10⁻³ s⁻¹ and 11 × 10⁻³ s⁻¹ for ADS and ECS, respectively. Agroinfiltration of leaves of Nicotiana bentamiana can be used to produce recombinant biosynthetic enzymes as exemplified by two sesquiterpene synthases from Artemisia annua in relatively high yields.

  6. Ptaquiloside Z, a new toxic unstable sesquiterpene glucoside from the neotropical bracken fern Pteridium aquilinum var. caudatum.

    Science.gov (United States)

    Castillo, U F; Ojika, M; Alonso-Amelot, M; Sakagami, Y

    1998-11-01

    Reversed-phase HPLC fractionation, monitored by brine shrimp bioassay, led to the isolation of a new illudane-type sesquiterpene glucoside, ptaquiloside Z (2), as well as the known bracken carcinogen ptaquiloside (1), from a bioactive aqueous extract of the neotropical bracken fern Pteridium aquilinum var. caudatum (Pteridaceae). The structure of ptaquiloside Z (2) was confirmed by spectroscopic analyses and chemical degradation. Both compounds exhibited similar toxicity (LC50 62.5 micrograms/ml at 24 h and LC50 7.8 micrograms/ml at 48 h) toward brine shrimp.

  7. Enhancement of Farnesyl Diphosphate Pool as Direct Precursor of Sesquiterpenes Through Metabolic Engineering of the Mevalonate Pathway in Saccharomyces cerevisiae

    DEFF Research Database (Denmark)

    Asadollahi, Mohammadali; Maury, Jerome; Schalk, M.

    2010-01-01

    , resulted in higher production of cubebol, a plant originating sesquiterpene, and increased squalene accumulation. Down-regulation of ERG9 by replacing its native promoter with the regulatable MET3 promoter, enhanced cubebol titers but simultaneous overexpression of tHMG1 and repression of ERG9 did...... not further improve cubebol production. Furtheremore, the concentrations of squalene and ergosterol were measured in the engineered strains. Unexpectedly, significant accumulation of squalene and restoring the ergosterol biosynthesis were observed in the ERG9 repressed strains transformed with the plasmids...

  8. Role of the Colletotrichum acutatum sesquiterpene synthase CaTPS in the biosynthesis of sesquiterpenoids

    DEFF Research Database (Denmark)

    Amby, Daniel Buchvaldt; Manczak, Tom; Petersen, Mikael Agerlin

    2016-01-01

    biosynthesis is performed by sesquiterpene synthases (TPS). Only a few TPSs have been functionally characterized from filamentous fungi and none from the genus Colletotrichum. Despite being an important fungal pathogen to agriculture, it is poorly understood at the molecular and chemical levels. The terpenoid......-humulene as one of the minor sesquiterpenoid products. These products were also secreted by the fungus in strawberry fruit medium along with several other sesquiterpenoids indicating other TPSs are active during in vitro growth. b-Caryophyllene and a-humulene are known cytotoxic products important for ecological...

  9. Biosynthesis of sesquiterpenes in grape berry exocarp of Vitis vinifera L.: evidence for a transport of farnesyl diphosphate precursors from plastids to the cytosol.

    Science.gov (United States)

    May, Bianca; Lange, B Markus; Wüst, Matthias

    2013-11-01

    The participation of the mevalonic acid (MVA) and 1-deoxy-d-xylulose 5-phosphate/2-C-methyl-d-erythritol-4-phosphate (DOXP/MEP) pathways in sesquiterpene biosynthesis of grape berries was investigated. There is an increasing interest in this class of terpenoids, since the oxygenated sesquiterpene rotundone was identified as the peppery aroma impact compound in Australian Shiraz wines. To investigate precursor supply pathway utilization, in vivo feeding experiments were performed with the deuterium labeled, pathway specific, precursors [5,5-(2)H2]-1-deoxy-d-xylulose and [5,5-(2)H2]-mevalonic acid lactone. Head Space-Solid Phase Micro Extraction-Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS) analysis of the generated volatile metabolites demonstrated that de novo sesquiterpene biosynthesis is mainly located in the grape berry exocarp (skin), with no detectable activity in the mesocarp (flesh) of the Lemberger variety. Interestingly, precursors from both the (primarily) cytosolic MVA and plastidial DOXP/MEP pathways were incorporated into grape sesquiterpenes in the varieties Lemberger, Gewürztraminer and Syrah. Our labeling data provide evidence for a homogenous, cytosolic pool of precursors for sesquiterpene biosynthesis, indicating that a transport of precursors occurs mostly from plastids to the cytosol. The labeling patterns of the sesquiterpene germacrene D were in agreement with a cyclization mechanism analogous to that of a previously cloned enantioselective (R)-germacrene D synthase from Solidago canadensis. This observation was subsequently confirmed by enantioselective GC-MS analysis demonstrating the exclusive presence of (R)-germacrene D, and not the (S)-enantiomer, in grape berries. Copyright © 2013 Elsevier Ltd. All rights reserved.

  10. Os estudos latinos no Brasil

    Directory of Open Access Journals (Sweden)

    Eduardo Tuffani

    2001-12-01

    Full Text Available Trata-se da importância do ensino do Latim no periodo colonial, enfatizando o papel da Companhia de Jesus. É discutido o longo processo da reorganização do ensino secundário e da implantação dos estudos superiores. Apresenta-se a época que vai do fim do secundário de tradição literária à fundação dos primeiros cursos de Letras e Filosofia. Faz-se um balanco dos estudos latinos de 1940 a 1970, período de sua maior vitalidade no Brasil, tratando da Lei Capanema de 1942 e da antiga Lei de Diretrizes e Bases da Educação Nacional. É fechado o panorama com o relato das atividades ate 1996, considerando o quadro por ocasião da nova LDB.

  11. The impact of sesquiterpene lactones and phenolics on sensory attributes: An investigation of a curly endive and escarole germplasm collection.

    Science.gov (United States)

    Filippo D'Antuono, L; Ferioli, Federico; Manco, Manuela Agata

    2016-05-15

    In the present study, curly endive (Cichorium endivia L. var. crispum) and escarole (Cichorium endivia L. var. latifolium) accessions were investigated for their sensory characters (bitterness, astringency and herbaceous flavour) and acceptance in relation to sesquiterpene lactone and phenolic content. Different facets of the perception of these sensory traits in relation to lactones and phenolics were brought out. Lactucopicrin and kaempferol malonyl glucoside were consistently related to bitterness, astringency and herbaceous flavour perceptions. Overall acceptance was significantly and inversely related mainly to bitterness. The generic statement that sesquiterpene lactones and phenolic compounds are determinants of bitterness and other related sensory characters does not seem to be fully consistent with our data, that indicated how the balance of different compounds affects these traits individually, in a rather complex manner, with a prevailing negative impact of phenolics. Bitter, astringent, and herbaceous perceptions were significantly affected by variety, with curly endive showing on average higher scores in comparison to escarole, with particular respect to bitterness. Copyright © 2015 Elsevier Ltd. All rights reserved.

  12. Further Study on Chemical Constituents of Parnassia wightiana Wall: Four New Dihydro-β-agarofuran Sesquiterpene Polyesters

    Directory of Open Access Journals (Sweden)

    Zhao-Feng Gao

    2015-04-01

    Full Text Available Four new (1–4, along with six known (5–10 dihydro-β-agarofuran sesquiterpene polyesters were isolated from the whole plants of Parnassia wightiana. The new compounds were structurally elucidated through spectroscopic analysis including UV (Ultraviolet Spectrum, IR (Infrared Spectrum, 1H-NMR (1Hydrogen-Nuclear Magnetic Resonance, 13C-NMR (13Carbon-Nuclear Magnetic Resonance, DEPT (Distortionless Enhancement by Polarization Transfer, 1H-1H COSY (1H-1H Correlation Spectroscopy, HSQC (Heteronuclear Single Quantum Coherence, HMBC (Heteronuclear Multiple Bond Correlation, NOESY (Nuclear Overhauser Enhancement Spectroscopy and HR-MS (High Resolution Mass Specttrum and their absolute configurations were proposed by comparison of NOESY spectra and specific optical rotations with those of known compounds and biosynthesis grounds. Compound 2 is the first sesquiterpene alkaloid isolated from this plant. New compounds 1–4 exhibited some cytotoxic activities against NB4, MKN-45 and MCF-7 cells at 20 μM and of which 4 showed the highest activity against NB4 and MKN-45 cells with inhibition rates of 85.6% and 30.5%, respectively.

  13. Non-Oxygenated Sesquiterpenes in the Essential Oil of Copaifera langsdorffii Desf. Increase during the Day in the Dry Season.

    Science.gov (United States)

    de Almeida, Luiz Fernando Rolim; Portella, Roberto de Oliveira; Bufalo, Jennifer; Marques, Márcia Ortiz Mayo; Facanali, Roselaine; Frei, Fernando

    2016-01-01

    The present study aimed to evaluate the effect of seasonal and diurnal events on the chemical profile of the essential oil obtained from the leaves of Copaifera langsdorffii Desf. This study was performed in a Brazilian savanna named Cerrado. We identified the best harvesting period for obtaining the highest amount of compounds used for commercial and industrial purposes. The chemical profile of the essential oils was evaluated by GC-FID and GC-MS, and the results were assessed through multivariate analyses. The data showed that the time of day and seasonal variations affect the quality of the essential oil obtained. Leaves harvested at the end of the day (5:00 pm) in the dry season resulted in richer essential oils with higher amounts of non-oxygenated sesquiterpenes. To the best of our knowledge, environmental conditions induce metabolic responses in the leaves of C. langsdorffii, which changes the patterns of sesquiterpene production. Therefore, these factors need to be considered to obtain better concentrations of bioactive compounds for pharmacological studies.

  14. The Effects of Selected Sesquiterpenes from Myrica rubra Essential Oil on the Efficacy of Doxorubicin in Sensitive and Resistant Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    Martin Ambrož

    2017-06-01

    Full Text Available β-caryophyllene oxide (CAO, α-humulene (HUM, trans-nerolidol (NER and valencene (VAL are constituents of the essential oil of Myrica rubra (MEO, which has significant antiproliferative effect in various cancer cell lines. In the present study, we compared the antiproliferative effect of these sesquiterpenes alone and in combination with the cytostatic drug doxorubicin (DOX in cancer cell lines with different sensitivity to DOX. Two ovarian cancer cell lines (sensitive A2780 and partly resistant SKOV3 and two lymphoblast cancer cell lines (sensitive CCRF/CEM and completely resistant CEM/ADR were used. The observed effects varied among sesquiterpenes and also differed in individual cell lines, with only VAL being effective in all the cell lines. A strong synergism of DOX with NER was found in the A2780 cells, while DOX acted synergistically with HUM and CAO in the SKOV3 cells. In the CCRF/CEM cells, a synergism of DOX with CAO and NER was observed. In resistant CEM/ADR cells, sesquiterpenes did not increase DOX efficacy, although they significantly increased accumulation of DOX (up to 10-times and rhodamine-123 (substrate of efflux transporter ABCB1 within cancer cells. In conclusion, the tested sesquiterpenes were able to improve DOX efficacy in the sensitive and partly resistant cancer cells, but not in cells completely resistant to DOX.

  15. Germacrene C synthase from Lycopersicon esculentum cv. VFNT cherry tomato: cDNA isolation, characterization, and bacterial expression of the multiple product sesquiterpene cyclase.

    Science.gov (United States)

    Colby, S M; Crock, J; Dowdle-Rizzo, B; Lemaux, P G; Croteau, R

    1998-03-03

    Germacrene C was found by GC-MS and NMR analysis to be the most abundant sesquiterpene in the leaf oil of Lycopersicon esculentum cv. VFNT Cherry, with lesser amounts of germacrene A, guaia-6,9-diene, germacrene B, beta-caryophyllene, alpha-humulene, and germacrene D. Soluble enzyme preparations from leaves catalyzed the divalent metal ion-dependent cyclization of [1-3H]farnesyl diphosphate to these same sesquiterpene olefins, as determined by radio-GC. To obtain a germacrene synthase cDNA, a set of degenerate primers was constructed based on conserved amino acid sequences of related terpenoid cyclases. With cDNA prepared from leaf epidermis-enriched mRNA, these primers amplified a 767-bp fragment that was used as a hybridization probe to screen the cDNA library. Thirty-one clones were evaluated for functional expression of terpenoid cyclase activity in Escherichia coli by using labeled geranyl, farnesyl, and geranylgeranyl diphosphates as substrates. Nine cDNA isolates expressed sesquiterpene synthase activity, and GC-MS analysis of the products identified germacrene C with smaller amounts of germacrene A, B, and D. None of the expressed proteins was active with geranylgeranyl diphosphate; however, one truncated protein converted geranyl diphosphate to the monoterpene limonene. The cDNA inserts specify a deduced polypeptide of 548 amino acids (Mr = 64,114), and sequence comparison with other plant sesquiterpene cyclases indicates that germacrene C synthase most closely resembles cotton delta-cadinene synthase (50% identity).

  16. Ectopic Terpene Synthase Expression Enhances Sesquiterpene Emission in Nicotiana attenuata without Altering Defense or Development of Transgenic Plants or Neighbors1[W

    Science.gov (United States)

    Schuman, Meredith C.; Palmer-Young, Evan C.; Schmidt, Axel; Gershenzon, Jonathan; Baldwin, Ian T.

    2014-01-01

    Sesquiterpenoids, with approximately 5,000 structures, are the most diverse class of plant volatiles with manifold hypothesized functions in defense, stress tolerance, and signaling between and within plants. These hypotheses have often been tested by transforming plants with sesquiterpene synthases expressed behind the constitutively active 35S promoter, which may have physiological costs measured as inhibited growth and reduced reproduction or may require augmentation of substrate pools to achieve enhanced emission, complicating the interpretation of data from affected transgenic lines. Here, we expressed maize (Zea mays) terpene synthase10 (ZmTPS10), which produces (E)-α-bergamotene and (E)-β-farnesene, or a point mutant ZmTPS10M, which produces primarily (E)-β-farnesene, under control of the 35S promoter in the ecological model plant Nicotiana attenuata. Transgenic N. attenuata plants had specifically enhanced emission of target sesquiterpene(s) with no changes detected in their emission of any other volatiles. Treatment with herbivore or jasmonate elicitors induces emission of (E)-α-bergamotene in wild-type plants and also tended to increase emission of (E)-α-bergamotene and (E)-β-farnesene in transgenics. However, transgenics did not differ from the wild type in defense signaling or chemistry and did not alter defense chemistry in neighboring wild-type plants. These data are inconsistent with within-plant and between-plant signaling functions of (E)-β-farnesene and (E)-α-bergamotene in N. attenuata. Ectopic sesquiterpene emission was apparently not costly for transgenics, which were similar to wild-type plants in their growth and reproduction, even when forced to compete for common resources. These transgenics would be well suited for field experiments to investigate indirect ecological effects of sesquiterpenes for a wild plant in its native habitat. PMID:25187528

  17. Citrus fruit flavor and aroma biosynthesis: isolation, functional characterization, and developmental regulation of Cstps1, a key gene in the production of the sesquiterpene aroma compound valencene.

    Science.gov (United States)

    Sharon-Asa, Liat; Shalit, Moshe; Frydman, Ahuva; Bar, Einat; Holland, Doron; Or, Etti; Lavi, Uri; Lewinsohn, Efraim; Eyal, Yoram

    2003-12-01

    Citrus fruits possess unique aromas rarely found in other fruit species. While fruit flavor is composed of complex combinations of soluble and volatile compounds, several low-abundance sesquiterpenes, such as valencene, nootkatone, alpha-sinensal, and beta-sinensal, stand out in citrus as important flavor and aroma compounds. The profile of terpenoid volatiles in various citrus species and their importance as aroma compounds have been studied in detail, but much is still lacking in our understanding of the physiological, biochemical, and genetic regulation of their production. Here, we report on the isolation, functional expression, and developmental regulation of Cstps1, a sesquiterpene synthase-encoding gene, involved in citrus aroma formation. The recombinant enzyme encoded by Cstps1 was shown to convert farnesyl diphosphate to a single sesquiterpene product identified as valencene by gas chromatography-mass spectrometry (GC-MS). Phylogenetic analysis of plant terpene synthase genes localized Cstps1 to the group of angiosperm sesquiterpene synthases. Within this group, Cstps1 belongs to a subgroup of citrus sesquiterpene synthases. Cstps1 was found to be developmentally regulated: transcript was found to accumulate only towards fruit maturation, corresponding well with the timing of valencene accumulation in fruit. Although citrus fruits are non-climacteric, valencene accumulation and Cstps1 expression were found to be responsive to ethylene, providing further evidence for the role of ethylene in the final stages of citrus fruit ripening. Isolation of the gene encoding valencene synthase provides a tool for an in-depth study of the regulation of aroma compound biosynthesis in citrus and for metabolic engineering for fruit flavor characteristics.

  18. Estudo multicasos sobre atividades inovativas

    Directory of Open Access Journals (Sweden)

    Sonia Regina Hierro Parolin

    2013-09-01

    Full Text Available Em estudos sobre competitividade argumenta-se que as empresas necessitam de estratégias baseadas em inovações e de capacidades internas em constantes e intensas transformações, não somente nos padrões tecnológicos, mas também no encadeamento do processo de gestão das atividades inovativas, como processos dinâmicos, não lineares e diversificados, em função de múltiplos fatores dos ambientes interno e externo. Para contribuir com essas discussões, neste artigo apresenta-se um estudo multicasos em quatro indústrias de médio e grande portes, de segmentos, densidades tecnológicas e históricos com inovações diferentes entre si. Como principais resultados, salientam-se o encadeamento das atividades inovativas como parte de uma estratégia organizacional para obter resultados com inovação e a afluência de todas as pessoas, e não somente as alocadas em pesquisa e desenvolvimento, para o cumprimento dessa estratégia.

  19. In vitro effect of essential oils and isolated mono- and sesquiterpenes on Leishmania major and Trypanosoma brucei.

    Science.gov (United States)

    Mikus, J; Harkenthal, M; Steverding, D; Reichling, J

    2000-05-01

    The effect of different essential oils as well as of isolated mono- and sesquiterpenes on the viability of bloodstream forms of Trypanosoma brucei, promastigotes of Leishmania major and human HL-60 cells was evaluated using the Almar Blue assay. Of the 12 essential oils and 8 terpenes investigated, only three essential oils, Melissa officinalis (balmmint) oil, Thymus vulgaris (thyme) oil, and Melaleuca alternifolia (tea tree) oil were about 50-fold and 80-fold more toxic to bloodstream forms of T. brucei than to HL-60 cells, respectively. Terpinen-4-ol, the main compound of the Australian tea tree oil, was even 1000-fold more toxic to trypanosomes than to the human cells. On the other hand, none of the essential oils and terpenes tested were more toxic to promastigotes of L. major than to HL-60 cells.

  20. Sesquiterpenes from the essential oil of Laurencia dendroidea (Ceramiales, Rhodophyta: isolation, biological activities and distribution among seaweeds

    Directory of Open Access Journals (Sweden)

    Vanessa Gressler

    2011-04-01

    Full Text Available Two known sesquiterpenes (1R*,2S*,3R*,5S*,8S*,9R*-2,3,5,9-tetramethyltricyclo[6.3.0.0(1,5]undecan-2-ol and (1S*,2S*,3S*,5S*,8S*,9S*-2,3,5,9-tetramethyltricyclo-[6.3.0.0(1,5]undecan-2-ol were isolated for the first time from the essential oil of the red seaweed Laurencia dendroidea collected in the Brazilian coast. These compounds were not active against eight bacteria strains and the yeast Candida albicans, but showed some antioxidant activity. Both compounds were also found in other seaweed species showing that they are not exclusive taxonomic markers to the genus Laurencia.

  1. 3-Ishwarone, a Rare Ishwarane Sesquiterpene from Peperomia scandens Ruiz & Pavon: Structural Elucidation through a Joint Experimental and Theoretical Study

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    Fernando M. dos S.

    2013-10-01

    Full Text Available 3-Ishwarone, (1, a sesquiterpene with a rare ishwarane skeleton, was isolated from Peperomia scandens Ruiz & Pavon (Piperaceae. Its structure was unambiguously determined by 1D- and 2D-NMR and infrared analyses, as well as by comparative theoretical studies which involved calculations of 13C-NMR chemical shifts, using the Density Functional Theory (DFT with the mPW1PW91 hybrid functional and Pople’s 6-31G(d basis set, and of vibrational frequencies, using the B3LYP hybrid functional and triple ζ Dunning’s correlation consistent basis set (cc-pVTZ, of (1 and three of its possible diastereomers, compounds 2–4.

  2. Computer-Aided Drug Design Using Sesquiterpene Lactones as Sources of New Structures with Potential Activity against Infectious Neglected Diseases

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    Chonny Herrera Acevedo

    2017-01-01

    Full Text Available This review presents an survey to the biological importance of sesquiterpene lactones (SLs in the fight against four infectious neglected tropical diseases (NTDs—leishmaniasis, schistosomiasis, Chagas disease, and sleeping sickness—as alternatives to the current chemotherapies that display several problems such as low effectiveness, resistance, and high toxicity. Several studies have demonstrated the great potential of some SLs as therapeutic agents for these NTDs and the relationship between the protozoal activities with their chemical structure. Recently, Computer-Aided Drug Design (CADD studies have helped increase the knowledge of SLs regarding their mechanisms, the discovery of new lead molecules, the identification of pharmacophore groups and increase the biological activity by employing in silico tools such as molecular docking, virtual screening and Quantitative-Structure Activity Relationship (QSAR studies.

  3. SF002-96-1, a new drimane sesquiterpene lactone from an Aspergillus species, inhibits survivin expression

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    Silke Felix

    2013-12-01

    Full Text Available Survivin, a member of the IAP (inhibitor of apoptosis gene family, is overexpressed in virtually all human cancers and is functionally involved in the inhibition of apoptosis, regulation of cell proliferation, metastasis and resistance to therapy. Because of its upregulation in malignancy, survivin has currently attracting considerable interest as a new target for anticancer therapy. In a screening of approximately 200 strains of imperfect fungi for the production of inhibitors of survivin promoter activity, a new drimane sesquiterpene lactone, SF002-96-1, was isolated from fermentations of an Aspergillus species. The compound inhibited survivin promoter activity in transiently transfected Colo 320 cells in a dose dependent manner with IC50 values of 3.42 µM (1.3 µg/mL. Moreover, it also reduced mRNA levels and protein synthesis of survivin and triggered apoptosis.

  4. Purpurides B and C, two new sesquiterpene esters from the aciduric fungus Penicillium purpurogenum JS03-21.

    Science.gov (United States)

    Wang, Hui; Wang, Yi; Liu, Peipei; Wang, Wei; Fan, Yaqin; Zhu, Weiming

    2013-07-01

    Purpurides B and C (1 and 2, resp.), two new sesquiterpene alcohol esters generated from a drimane-type sesquiterpenoid lactone and an amino acid, together with two known analogs, berkedrimane B (3) and purpuride (4), were isolated from the aciduric fungus Penicillium purpurogenum JS03-21. Their structures were elucidated by spectroscopic analysis, X-ray single-crystal diffraction, and application of Marfey's method. Compounds 1-4 showed modest antifungal activities against Candida albicans with MIC values in the range of 1.2-3.3 μM. Compounds 1 and 2 showed moderate antimicrobial activities against Enterobacter aerogenes and Pseudomonas aeruginosa with MIC values in the range of 1.2-2.6 μM. Copyright © 2013 Verlag Helvetica Chimica Acta AG, Zürich.

  5. Gas-phase rate coefficients of the reaction of ozone with four sesquiterpenes at 295 ± 2 K.

    Science.gov (United States)

    Richters, Stefanie; Herrmann, Hartmut; Berndt, Torsten

    2015-05-07

    The rate coefficients of the reaction of ozone with the four atmospherically relevant sesquiterpenes β-caryophyllene, α-humulene, α-cedrene and isolongifolene were investigated at 295 ± 2 K and atmospheric pressure by at least two independent experimental investigations for each reaction. Relative rate experiments were carried out in a flow tube using two different experimental approaches with GC-MS detection (RR 1) and PTR-MS analysis (RR 2) as the analytical techniques. Absolute rate coefficients were determined in a stopped-flow experiment following the ozone depletion by means of UV spectroscopy. The average rate coefficients from the combined investigations representing the mean values of the different experimental methods are (unit: cm(3) molecule(-1) s(-1)): k(O3+β-caryophyllene) = (1.1 ± 0.3) × 10(-14) (methods: RR 1, RR 2, absolute), k(O3+α-humulene) = (1.2 ± 0.3) × 10(-14) (RR 1, RR 2), k(O3+α-cedrene) = (1.7 ± 0.5) × 10(-16) (RR 2, absolute) and k(O3+isolongifolene) = (1.1 ± 0.5) × 10(-17) (RR 2, absolute). The high ozonolysis rate coefficients for β-caryophyllene and α-humulene agree well with the results by Shu and Atkinson (Int. J. Chem. Kinet., 1994, 26) and lead to short atmospheric lifetimes of about two minutes with respect to the ozone reaction. The relatively small rate coefficients for α-cedrene and isolongifolene differ from the available literature values by a factor of about 2.5-6. Possible reasons for the deviations are discussed. Finally, calibrated sesquiterpene FT-IR spectra were recorded for the first time.

  6. Use of self-organizing maps and molecular descriptors to predict the cytotoxic activity of sesquiterpene lactones.

    Science.gov (United States)

    Fernandes, Mariane B; Scotti, Marcus T; Ferreira, Marcelo J P; Emerenciano, Vicente P

    2008-10-01

    Some sesquiterpene lactones (SLs) are the active compounds of a great number of traditionally medicinal plants from the Asteraceae family and possess considerable cytotoxic activity. Several studies in vitro have shown the inhibitory activity against cells derived from human carcinoma of the nasopharynx (KB). Chemical studies showed that the cytotoxic activity is due to the reaction of alpha,beta-unsaturated carbonyl structures of the SLs with thiols, such as cysteine. These studies support the view that SLs inhibit tumour growth by selective alkylation of growth-regulatory biological macromolecules, such as key enzymes, which control cell division, thereby inhibiting a variety of cellular functions, which directs the cells into apoptosis. In this study we investigated a set of 55 different sesquiterpene lactones, represented by 5 skeletons (22 germacranolides, 6 elemanolides, 2 eudesmanolides, 16 guaianolides and nor-derivatives and 9 pseudoguaianolides), in respect to their cytotoxic properties. The experimental results and 3D molecular descriptors were submitted to Kohonen self-organizing map (SOM) to classify (training set) and predict (test set) the cytotoxic activity. From the obtained results, it was concluded that only the geometrical descriptors showed satisfactory values. The Kohonen map obtained after training set using 25 geometrical descriptors shows a very significant match, mainly among the inactive compounds (approximately 84%). Analyzing both groups, the percentage seen is high (83%). The test set shows the highest match, where 89% of the substances had their cytotoxic activity correctly predicted. From these results, important properties for the inhibition potency are discussed for the whole dataset and for subsets of the different structural skeletons.

  7. Colestase neonatal prolongada: estudo prospectivo

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    PRADO Elizabeth Teixeira Mendes Livramento

    1999-01-01

    Full Text Available Em razão da urgência de se decidir por um tratamento clínico ou por uma intervenção cirúrgica imediata, o estudo da colestase neonatal prolongada envolve dois objetivos básicos: o diagnóstico diferencial entre atresia biliar e hepatite neonatal e a pesquisa dos agentes etiológicos associados. Desta maneira, através de estudo prospectivo desenvolvido na década de 1970, foram avaliadas 77 crianças portadoras de colestase neonatal prolongada para estabelecer o diagnóstico diferencial entre atresia biliar e hepatite neonatal e, numa segunda fase, 108 crianças, visando esclarecer a etiopatogenia da colestase neonatal prolongada. Os resultados do diagnóstico diferencial revelaram que, dos 18 atributos avaliados, apenas oito mostraram-se bons indicadores de atresia biliar, em ordem decrescente: ductos proliferados (espaço-porta, fibrose (espaço-porta, colestase (espaço-porta, cor das fezes -- acolia, hepatomegalia, colestase canalicular (lóbulo, infiltrado (espaço-porta, células gigantes (lóbulo. Estes oito atributos foram então compostos, mediante uma ponderação, em um único indicador de grande poder discriminativo, capaz de decidir o diagnóstico diferencial em 99% dos casos. Quanto à etiopatogenia, registrou-se: vírus rubéola 0%, vírus herpes simples 0%, listeriose 0%, citomegalovirose 2,2%, vírus hepatite B 2,4%, toxoplasmose 2,8%, deficiência de alfa-1-antitripsina 13,1%, sífilis 21,1 %, auto-anticorpos hepáticos 58,4%. O trabalho desenvolvido mostra que as 8 variáveis mais decisivas, como indicadoras diferenciais entre atresia biliar e hepatite neonatal, permanecem como índices fundamentais, auxiliando, em conjunto com novos métodos diagnósticos, na composição de uma estratégia multifatorial cada vez menos invasiva e mais precisa. O estudo da etiopatogenia, dependente das condições epidemiológicas locais e da época, com a introdução de novos métodos diagnósticos, torna-se atualmente cada vez mais

  8. In vitro analysis of the anthelmintic activity of forage chicory (Cichorium intybus L.) sesquiterpene lactones against a predominantly Haemonchus contortus egg population.

    Science.gov (United States)

    Foster, Joyce G; Cassida, Kimberly A; Turner, Kenneth E

    2011-08-25

    The anthelmintic activity of chicory (Cichorium intybus L.) herbage has been attributed to sesquiterpene lactones. Chicory leaves contain significant amounts of lactucin (LAC), 8-deoxylactucin (DOL), and lactucopicrin (LPIC), but the proportions of these three sesquiterpene lactones vary among forage chicory cultivars. To determine whether the individual compounds differ in anthelmintic activity, we prepared sesquiterpene lactone-enriched extracts from leaves of two forage chicory cultivars, 'Grasslands Puna' (Puna) and 'Forage Feast', and tested their effects on the hatching of a predominantly Haemonchus contortus egg population. The dominant constituents in the Puna and Forage Feast extracts were DOL and LAC, respectively; LPIC concentrations in the two extracts were similar. Extracts from both cultivars inhibited egg hatching at all concentrations tested (P<0.001), but there were significant differences in egg responses to the two extracts (P<0.001). With Puna, egg hatching decreased sharply in a linear fashion when the combined LAC, DOL, and LPIC concentrations ranged from 0 to 5.0mg/ml. A biphasic effect on egg hatching occurred with the Forage Feast extract. The fraction of eggs that hatched decreased gradually to 65% as the sesquiterpene lactone concentrations increased from 0 to 6.7 mg/ml. Treatment with higher concentrations resulted in a sharp decline in egg hatchability. Concentrations of sesquiterpene lactones required for 50% lethality were determined by probit dose-effect analysis to be 2.6 mg/ml (95% confidence interval: 2.4-2.8 mg/ml) for the Puna extract and 6.4 mg/ml (95% confidence interval: 5.9-7.2mg/ml) for the Forage Feast extract (P<0.0001). These concentrations provided 1.3 and 1.5mg/ml of DOL and 0.8 and 3.9 mg/ml of LAC for Puna and Forage Feast extracts, respectively. Results suggest that LAC has minimal effect on egg hatching and that DOL or other constituent(s) in the extracts is inhibitory. Quantitative analyses of free sesquiterpene

  9. Sesquiterpenes and other constituents from leaves of Pterodon pubescens Benth (Leguminosae); Sesquiterpenos e outros constituintes das folhas de Pterodon pubescens Benth (Leguminosae)

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, Mayker Lazaro Dantas; Garcez, Fernanda Rodrigues; Garcez, Walmir Silva, E-mail: walmir.garcez@ufms.br [Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS (Brazil). Instituto de Quimica; Abot, Alfredo Raul [Universidade Estadual de Mato Grosso do Sul (UEMS), Aquidauana, MS (Brazil)

    2014-05-15

    In addition to β-sitosterol, stigmasterol, phaeophitin A, luteolin, kaempferol, quercetin, (+)-catechin, quercetin-3-O-α-L-rhamnopyranoside, rutin, and p-hydroxy-benzoic acid, six known sesquiterpenes, namely (rel)-2β,6β-epoxy-5β-hydroxy-isodaucane, oplopanone, 1β,6α-dihydroxy-4(15)-eudesmene, caryophyllene oxide, α-cadinol, and spathulenol, were isolated from the leaves of Pterodon pubescens (Leguminosae) growing in the Cerrado of Mato Grosso do Sul, Brazil. The (rel)-2β,6β-epoxy-5β-hydroxy-isodaucane corresponds to the correct structure of homalomenol D. The sesquiterpene oplopanone, which bears a modified cadinane skeleton, is being reported for the first time in this genus. The structures of the compounds were determined on the basis of spectral data (MS, IR, and NMR-1D and 2D) and subsequent comparison with data reported in the literature. (author)

  10. Temporal analysis of sesquiterpene emissions from manuka and phoebe oil lures and efficacy for attraction of Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae).

    Science.gov (United States)

    Kendra, Paul E; Niogret, Jerome; Montgomery, Wayne S; Sanchez, Jorge S; Deyrup, Mark A; Pruett, Grechen E; Ploetz, Randy C; Epsky, Nancy D; Heath, Robert R

    2012-04-01

    Redbay ambrosia beetle, Xyleborus glabratus Eichhoff, is an exotic wood-borer that vectors the fungal agent (Raffaelea lauricola) responsible for laurel wilt. Laurel wilt has had severe impact on forest ecosystems in the southeastern United States, killing a large proportion of native Persea trees, particularly redbay (P. borbonia) and swampbay (P. palustris), and currently poses an economic threat to avocado (P. americana) in Florida. To control the spread of this lethal disease, effective attractants are needed for early detection of the vector. Two 12-wk field tests were conducted in Florida to evaluate efficacy and longevity of manuka and phoebe oil lures, and to relate captures of X. glabratus to release rates of putative sesquiterpene attractants. Two trap types were also evaluated, Lindgren funnel traps and sticky panel traps. To document lure emissions over time, a separate set of lures was aged outdoors for 12 wk and sampled periodically to quantify volatile sesquiterpenes using super-Q adsorbant and gas chromatography-mass spectroscopy analysis. Phoebe lures captured significantly more X. glabratus than manuka lures, and sticky traps captured more beetles than funnel traps. Phoebe lures captured X. glabratus for 10-12 wk, but field life of manuka lures was 2-3 wk. Emissions of alpha-copaene, alpha-humulene, and cadinene were consistently higher from phoebe lures, particularly during the 2-3 wk window when manuka lures lost efficacy, suggesting that these sesquiterpenes are primary kairomones used by host-seeking females. Results indicate that the current monitoring system is suboptimal for early detection of X. glabratus because of rapid depletion of sesquiterpenes from manuka lures.

  11. Understanding the Constitutive and Induced Biosynthesis of Mono- and Sesquiterpenes in Grapes (Vitis vinifera): A Key to Unlocking the Biochemical Secrets of Unique Grape Aroma Profiles.

    Science.gov (United States)

    Schwab, Wilfried; Wüst, Matthias

    2015-12-16

    The present review integrates current knowledge on mono- and sesquiterpenes in grapes with a special focus on biochemical and physiological aspects. Recent research has impressively shown the prominence of terpenoid metabolism in grapevine (Vitis sp). The 69 putatively functional mono- and sesquiterpene synthases that were identified by the analysis of the updated 12-fold sequencing and assembly of the grapevine genome deliver the scaffolds for structural diversity and display a surprising expansion of the terpene synthase (TPS) gene family in grapevine when compared to other plants like Arabidopsis thaliana (32 TPS). While monoterpenes occur as highly functionalized compounds and are stored as their corresponding glycoconjugates in berry tissues, sesquiterpenes are mainly present as unsaturated hydrocarbons and accumulate in the epicuticular wax layer of intact berries. Interestingly, both groups of terpenes appear to be involved as volatile organic compounds in plant defense and their biosynthesis is enhanced via the jasmonic acid signaling pathway. These novel aspects will help to understand how environmental cues affect the genes and enzymes of various metabolic pathways of relevant wine aroma compounds with numerous links to enology and wine flavor chemistry.

  12. Sesquiterpene Lactone Composition and Cellular Nrf2 Induction of Taraxacum officinale Leaves and Roots and Taraxinic Acid β-d-Glucopyranosyl Ester.

    Science.gov (United States)

    Esatbeyoglu, Tuba; Obermair, Betina; Dorn, Tabea; Siems, Karsten; Rimbach, Gerald; Birringer, Marc

    2017-01-01

    Taraxacum officinale, the common dandelion, is a plant of the Asteraceae family, which is used as a food and medical herb. Various secondary plant metabolites such as sesquiterpene lactones, triterpenoids, flavonoids, phenolic acids, coumarins, and steroids have been described to be present in T. officinale. Dandelion may exhibit various health benefits, including antioxidant, anti-inflammatory, and anticarcinogenic properties. We analyzed the leaves and roots of the common dandelion (T. officinale) using high-performance liquid chromatography/mass spectrometry to determine its sesquiterpene lactone composition. The main compound of the leaf extract taraxinic acid β-d-glucopyranosyl ester (1), a sesquiterpene lactone, was isolated and the structure elucidation was conducted by nuclear magnetic resonance spectrometry. The leaf extract and its main compound 1 activated the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) in human hepatocytes more significantly than the root extract. Furthermore, the leaf extract induced the Nrf2 target gene heme oxygenase 1. Overall, present data suggest that compound 1 may be one of the active principles of T. officinale.

  13. Virtual Screening of Plant Volatile Compounds Reveals a High Affinity of Hylamorpha elegans (Coleoptera: Scarabaeidae) Odorant-Binding Proteins for Sesquiterpenes From Its Native Host

    Science.gov (United States)

    Palma-Millanao, Rubén; Yáñez, Osvaldo; Rojas, Maximiliano; Mutis, Ana; Venthur, Herbert; Quiroz, Andrés; Ramírez, Claudio C.

    2016-01-01

    Hylamorpha elegans (Burmeister) is a native Chilean scarab beetle considered to be a relevant agricultural pest to pasture and cereal and small fruit crops. Because of their cryptic habits, control with conventional methods is difficult; therefore, alternative and environmentally friendly control strategies are highly desirable. The study of proteins that participate in the recognition of odorants, such as odorant-binding proteins (OBPs), offers interesting opportunities to identify new compounds with the potential to modify pest behavior and computational screening of compounds, which is commonly used in drug discovery, may help to accelerate the discovery of new semiochemicals. Here, we report the discovery of four OBPs in H. elegans as well as six new volatiles released by its native host Nothofagus obliqua (Mirbel). Molecular docking performed between OBPs and new and previously reported volatiles from N. obliqua revealed the best binding energy values for sesquiterpenic compounds. Despite remarkable divergence at the amino acid level, three of the four OBPs evaluated exhibited the best interaction energy for the same ligands. Molecular dynamics investigation reinforced the importance of sesquiterpenes, showing that hydrophobic residues of the OBPs interacted most frequently with the tested ligands, and binding free energy calculations demonstrated van der Waals and hydrophobic interactions to be the most important. Altogether, the results suggest that sesquiterpenes are interesting candidates for in vitro and in vivo assays to assess their potential application in pest management strategies. PMID:27012867

  14. The Influence of Sesquiterpenes from Myrica rubra on the Antiproliferative and Pro-Oxidative Effects of Doxorubicin and Its Accumulation in Cancer Cells

    Directory of Open Access Journals (Sweden)

    Martin Ambrož

    2015-08-01

    Full Text Available The sesquiterpenes β-caryophyllene, β-caryophyllene oxide (CAO, α-humulene (HUM, trans-nerolidol (NER, and valencene (VAL are substantial components of the essential oil from Myrica rubra leaves which has exhibited significant antiproliferative effects in several intestinal cancer cell lines, with CaCo-2 cells being the most sensitive. The present study was designed to evaluate the effects of these sesquiterpenes on the efficacy and toxicity of the anticancer drug doxorubicin (DOX in CaCo-2 cancer cells and in primary culture of rat hepatocytes. Our results showed that HUM, NER, VAL and CAO inhibited proliferation of CaCo-2 cancer cells but they did not affect the viability of hepatocytes. CAO, NER and VAL synergistically potentiated the efficacy of DOX in cancer cells killing. All sesquiterpenes exhibited the ability to selectively increase DOX accumulation in cancer cells and did not affect DOX concentration in hepatocytes. Additionally, CAO and VAL were able to increase the pro-oxidative effect of DOX in CaCo-2 cells. Moreover, CAO mildly ameliorated DOX toxicity in hepatocytes. Based on all results, CAO seems to be the most promising compound for further testing.

  15. The plant sesquiterpene germacrene D specifically activates a major type of antennal receptor neuron of the tobacco budworm moth Heliothis virescens.

    Science.gov (United States)

    Røstelien, T; Borg-Karlson, A K; Fäldt, J; Jacobsson, U; Mustaparta, H

    2000-04-01

    Plants release hundreds of volatiles that are important in interactions with insects or other organisms. However, knowledge is scarce as to which of the compounds are detected by the organism's olfactory receptor neurons. In the present study, single receptor neurons on the antennae of the tobacco budworm moth, Heliothis virescens, were screened for their sensitivities to naturally produced plant volatiles by the use of gas chromatography linked to electrophysiological recordings from single cells (GC-SCR). Plant volatiles, collected by aeration of host and non-host plants, were tested on each receptor neuron via parallel GC-columns. Thus, simultaneous recordings of the gas chromatogram and the neuron responses to each component were obtained. One type of receptor neuron, appearing in 80% of all experiments, responded with high sensitivity and selectivity to one particular component, present in host as well as non-host mixtures. The component, identified as a sesquiterpene hydrocarbon by linked gas chromatography-mass spectrometry, was isolated from a sesquiterpene fraction of cubebe oil and identified by NMR as germacrene D. The purified compound was then re-tested via gas chromatography on the same receptor neuron type, verifying the identification. A weaker response to another sesquiterpene hydrocarbon was also recorded.

  16. Coupling gene regulatory patterns to bioprocess conditions to optimize synthetic metabolic modules for improved sesquiterpene production in yeast.

    Science.gov (United States)

    Peng, Bingyin; Plan, Manuel R; Carpenter, Alexander; Nielsen, Lars K; Vickers, Claudia E

    2017-01-01

    Assembly of heterologous metabolic pathways is commonly required to generate microbial cell factories for industrial production of both commodity chemicals (including biofuels) and high-value chemicals. Promoter-mediated transcriptional regulation coordinates the expression of the individual components of these heterologous pathways. Expression patterns vary during culture as conditions change, and this can influence yeast physiology and productivity in both positive and negative ways. Well-characterized strategies are required for matching transcriptional regulation with desired output across changing culture conditions. Here, constitutive and inducible regulatory mechanisms were examined to optimize synthetic isoprenoid metabolic pathway modules for production of trans -nerolidol, an acyclic sesquiterpene alcohol, in yeast. The choice of regulatory system significantly affected physiological features (growth and productivity) over batch cultivation. Use of constitutive promoters resulted in poor growth during the exponential phase. Delaying expression of the assembled metabolic modules using the copper-inducible CUP1 promoter resulted in a 1.6-fold increase in the exponential-phase growth rate and a twofold increase in productivity in the post-exponential phase. However, repeated use of the CUP1 promoter in multiple expression cassettes resulted in genetic instability. A diauxie-inducible expression system, based on an engineered GAL regulatory circuit and a set of four different GAL promoters, was characterized and employed to assemble nerolidol synthetic metabolic modules. Nerolidol production was further improved by 60% to 392 mg L -1 using this approach. Various carbon source systems were investigated in batch/fed-batch cultivation to regulate induction through the GAL system; final nerolidol titres of 4-5.5 g L -1 were achieved, depending on the conditions. Direct comparison of different transcriptional regulatory mechanisms clearly demonstrated that

  17. Disturbios da olfacao: estudo retrospectivo

    Directory of Open Access Journals (Sweden)

    Luciano Lobato Gregorio

    2014-01-01

    Full Text Available Introdução: O olfato, fenômeno subjetivo de grande importância, é pouco compreendido e estudado no ser humano. Médicos com maior conhecimento sobre os distúrbios desse sentido tendem a considerar a doença mais importante e manejar melhor o diagnóstico e o tratamento. Objetivo: Descrever a amostra dos pacientes com queixa principal de distúrbios do olfato e mostrar a experiência do serviço no manejo e tratamento. Delineamento: Estudo retrospectivo de coorte histórica com corte transversal. Materiais e métodos: Descrição da amostra e avaliação de resposta ao tratamento de pacientes com queixa principal de hiposmia ou anosmia atendidos no ambulatório de Rinologia no período de janeiro de 2005 a outubro de 2011. Resultados: Dos 38 pacientes com distúrbio da olfação, 68,4% dos pacientes apresentaram queixa de hiposmia e 31,5% de anosmia, com duração média de 30,8 meses. Os diagnósticos etiológicos principais foram idiopática (31,5%, rinopatia alérgica (28,9% e RSC com pólipos (10,5%. As respostas ao tratamento com corticosteroide tópico e ácido alfa-lipoico foram variáveis, assim como na literatura. Conclusão: Maior importância deve ser dada aos distúrbios do olfato na prática do otorrinolaringologista, uma vez que o diagnóstico diferencial é amplo e pode trazer grande morbidade ao paciente, com impacto na sua qualidade de vida.

  18. Um estudo sobre o processo administrativo

    OpenAIRE

    Blasi, Ana Cristina Ferro

    1995-01-01

    Dissertação(mestrado) - Universidade Federal de Santa Catarina, Centro de Ciencias Juridicas Análise do fenômeno da processualização da atividade administrativa, com ênfase ao estudo do processo administrativo e dos princípios a ele inerentes, além do estudo da possibilidade de aplicação do princípio da legitimidade como norteador do exercício da atividade administrativa.

  19. The in vitro antileishmanial activity of essential oil from Aloysia gratissima and guaiol, its major sesquiterpene against Leishmania amazonensis.

    Science.gov (United States)

    Garcia, Maria Carolina Freitas; Soares, Deivid Costa; Santana, Raissa Couto; Saraiva, Elvira Maria; Siani, Antonio Carlos; Ramos, Mônica Freiman S; Danelli, Maria das Graças Miranda; Souto-Padron, Thaïs Cristina; Pinto-da-Silva, Lucia H

    2018-01-21

    Leishmaniases is a tropical disease caused by protozoa of the genus Leishmania for which the current treatment is expensive, besides increasing reports of parasite resistance. This study investigated the anti-Leishmania amazonensis activity of the essential oil from Aloysia gratissima (AgEO) and guaiol, the major sesquiterpene constituent in the oil. Our results showed that AgEO killed promastigotes and intracellular amastigotes at an IC50 of 25 and 0·16 µg mL-1, respectively, while guaiol killed amastigotes at an IC50 of 0·01 µg mL-1. Both AgEO and guaiol were safe for macrophages up to 100 µg mL-1, as evaluated by the dehydrogenase activity, membrane integrity and phagocytic capacity. AgEO and guaiol did not induce nitrite oxide (NO) in resting macrophages and inhibited the production of NO in lipopolysaccharide-stimulated macrophages. The ultrastructural analysis suggested that AgEO and guaiol act directly on parasites, affecting promastigotes kinetoplast, mitochondrial matrix and plasma membrane. Together, these results pointed out that AgEO and guaiol could be promising candidates to develop anti-Leishmania drugs.

  20. Isolation and validated HPTLC analysis of four cytotoxic compounds, including a new sesquiterpene from aerial parts of Plectranthus cylindraceus.

    Science.gov (United States)

    Amina, Musarat; Alam, Perwez; Parvez, Mohammad K; Al-Musayeib, Nawal M; Al-Hwaity, Samerah A; Al-Rashidi, Nada S; Al-Dosari, Mohammed S

    2018-04-01

    We report isolation of a new sesquiterpene (compound 2: plectranol A) along with three known compounds (1: maaliol, 3: penduletin and 4: chrysosplenol D) from the aerial parts of Plectranthus cylindraceus Hoechst. Ex. Benth ethyl acetate extract (PCEAE). Their structures were established by UV, IR, NMR ( 1 H & 13 C), HRESIMS and HSQC methods. The MTT cell proliferation assay of the compounds and the extract performed on human breast, skin and cervical cancer cell lines showed high toxicity, and their IC 50 (μg/mL) values were determined. The validated HPTLC analyses of maaliol and PCEAE (method I) and, penduletin, chrysosplenol D and PCEAE (method II) furnished sharp and compact peaks. The estimated high contents (μg/mg) of maaliol (17.06), penduletin (39.36) and chrysosplenol D (31.66) in PCEAE approved the plant's cytotoxic property. Since, P. cylindraceus contains a variety of cytotoxic terpenoids and flavonoids; our data warrant its further phytochemical and biological characterisation towards developing promising anti-cancer drugs.

  1. Quantitative HPLC analysis of sesquiterpene lactones and determination of chemotypes in Eremanthus seidelii MacLeish and Schumacher (Asteraceae)

    Energy Technology Data Exchange (ETDEWEB)

    Sakamoto, Humberto T. [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Filosofia, Ciencias e Letras. Dept. de Quimica; Gobbo-Neto, Leonardo; Lopes, Norberto P.; Lopes, Joao L.C. [Sao Paulo Univ., Ribeirao Preto, SP (Brazil). Faculdade de Ciencias Farmaceuticas. Dept. de Fisica e Quimica]. E-mail: joaoluis@usp.br; npelopes@fcfrp.usp.br; Cavalheiro, Alberto J. [UNESP, Araraquara, SP (Brazil). Inst. de Quimica

    2005-11-15

    anthus seidelii MacLeish and Schumacher has a restricted occurrence to the Brazilian 'cerrado' surrounding the Furnas (MG) reservoir, in environments that have been seriously damaged by human activity. The present phytochemical investigation reveals that the sesquiterpene lactones (SL) 4{beta},5-dihydro-2',3'-dihydroxy-15-desoxy-goyazensolide (1) and 4{beta},5-dihydro-1',2'-epoxy-eremantholide-C (2) are the major secondary metabolites in E. seidelii leaves, and an HPLC method was developed for their quantitative analysis. HPLC analysis showed no significant seasonal variation in the concentrations of both SL. No qualitative differences were found in the SL patterns of all individuals sampled. However, there is a different SL quantitative pattern among the plants analyzed, pointing to the existence of three quantitative chemotypes of this species, with differences possibly originating from the activity of the enzymes that cyclize the goyazensolide type SL (1) to a eremantholide type SL (2). (author)

  2. Structure and cytotoxic activity of sesquiterpene glycoside esters from Calendula officinalis L.: Studies on the conformation of viridiflorol.

    Science.gov (United States)

    D'Ambrosio, Michele; Ciocarlan, Alexandru; Colombo, Elisa; Guerriero, Antonio; Pizza, Cosimo; Sangiovanni, Enrico; Dell'Agli, Mario

    2015-09-01

    Topic applications of Calendula officinalis L. lipophilic extracts are used in phytotherapy to relieve skin inflammatory conditions whereas infusions are used as a remedy for gastric complaints. Such a different usage might be explained by some cytotoxicity of lipophilic extracts at gastric level but little is known about this. Therefore, we screened the CH2Cl2 extract from the flowers of C. officinalis by MTT and LDH assays in human epithelial gastric cells AGS. This bioassay-oriented approach led to the isolation of several sesquiterpene glycosides which were structurally characterized by spectroscopic measurements, chemical reactions and MM calculations. The conformational preferences of viridiflorol fucoside were established and a previously assigned stereochemistry was revised. The compounds 1a, 2a and 3f showed comparably high cytotoxicity in the MTT assays, whereas the effect on LDH release was lower. Our study provides new insights on the composition of C. officinalis extracts of medium polarity and identifies the main compounds that could be responsible for cytotoxic effects at gastric level. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Actions of Artemisia vulgaris extracts and isolated sesquiterpene lactones against receptors mediating contraction of guinea pig ileum and trachea.

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    Natividad, Gaudencio M; Broadley, Kenneth J; Kariuki, Benson; Kidd, Emma J; Ford, William R; Simons, Claire

    2011-09-01

    The present study evaluates the Philippine medicinal plant Artemisia vulgaris for antagonistic activity at selected biogenic amine receptors on smooth muscle of the airways and gastrointestinal tract in order to explain its traditional use in asthma and hyperactive gut. The antagonistic activity of chloroform crude extract (AV-CHCl(3)) and methanol crude extract (AV-MeOH) of Artemisia vulgaris was studied against concentration-response curves for contractions of the guinea pig ileum and trachea to 5-hydroxytrptamine (5-HT(2) receptors), methacholine (M(3) muscarinic receptors), histamine (H(1) receptors) and β-phenylethylamine (trace amine-associated receptors, TAAR1). The Artemisia vulgaris chloroform (AV-CHCl(3)) and methanol (AV-MeOH) extract showed histamine H1 antagonism in the ileum and trachea. Further analysis of AV-CHCl(3) isolated two major components, yomogin and 1,2,3,4-diepoxy-11(13)-eudesmen-12,8-olide. Yomogin, a sesquiterpene lactone, exhibited a novel histamine H1 receptor antagonism in the ileum. The presence of a specific, competitive histamine receptor antagonist and smooth muscle relaxant activity in Artemisia vulgaris extracts on the smooth muscle in ileum and trachea explains its traditional use in the treatment of asthma and hyperactive gut. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  4. Bisthiodiketopiperazines and Acorane Sesquiterpenes Produced by the Marine-Derived Fungus Penicillium adametzioides AS-53 on Different Culture Media.

    Science.gov (United States)

    Liu, Yang; Li, Xiao-Ming; Meng, Ling-Hong; Jiang, Wen-Li; Xu, Gang-Ming; Huang, Cai-Guo; Wang, Bin-Gui

    2015-06-26

    Chemical investigation of the marine-sponge-derived fungus Penicillium adametzioides AS-53 resulted in the identification of two new bisthiodiketopiperazine derivatives, adametizines A (1) and B (2), from cultivation in a liquid potato-dextrose broth (PDB) culture medium, whereas two new acorane sesquiterpenes, adametacorenols A (3) and B (4), were isolated from a rice solid culture medium. The structures of these compounds were elucidated on the basis of spectroscopic analysis. The absolute configuration of compound 1 was determined by X-ray crystallographic analysis, and that of 3 was determined by modified Mosher's method. Compound 1 exhibited lethality against brine shrimp (Artemia salina) with an LD50 value of 4.8 μM and inhibitory activities against Staphyloccocus aureus, Aeromonas hydrophilia, Vibrio spp. V. harveyi and V. parahaemolyticus, and Gaeumannomyces graminis with minimum inhibitory concentration values of 8, 8, 32, 8, and 16 μg/mL, respectively. Chlorination at C-7 significantly increased the brine shrimp lethality and antimicrobial activity of the bisthiodiketopiperazines.

  5. Skin penetration behaviour of sesquiterpene lactones from different Arnica preparations using a validated GC-MSD method.

    Science.gov (United States)

    Wagner, Steffen; Merfort, Irmgard

    2007-01-04

    Preparations of Arnica montana L. are widely used for the topical treatment of inflammatory diseases. The anti-inflammatory activity is mainly attributed to their sesquiterpene lactones (SLs) from the helenalin and 11alpha,13-dihydrohelenalin type. To study the penetration kinetics of SLs in Arnica preparations, a stripping method with adhesive tape and pig skin as a model was used. For the determination of SLs in the stripped layers of the stratum corneum (SC), a gas chromatography/mass spectrometry method was developed and validated. Thereby the amount of helenalin derivatives was calculated as helenalin isobutyrate, and 11alpha,13-dihydrohelenalin derivatives as 11alpha,13-dihydrohelenalin methacrylate. This GC-MSD method is suitable also to determine low amounts of SLs in Arnica preparations. The penetration behaviour of one gel preparation and two ointment preparations was investigated. The SLs of all preparations show a comparable penetration in and a permeation through the stratum corneum, the uppermost part of the skin. Interestingly, the gel preparation showed a decrease of the penetration rate over 4h, whereas the penetration rate of ointments kept constant over time. Moreover, we could demonstrate that the totally penetrated amount of SLs only depends on the kind of the formulation and of the SLs-content in the formulation but not on the SLs composition or on the used extraction agent.

  6. Semisynthesis and in vitro cytotoxic evaluation of new analogues of 1-O-acetylbritannilactone, a sesquiterpene from Inula britannica.

    Science.gov (United States)

    Dong, Shuai; Tang, Jiang-Jiang; Zhang, Cheng-Chen; Tian, Jun-Mian; Guo, Jun-Tao; Zhang, Qiang; Li, He; Gao, Jin-Ming

    2014-06-10

    Semisynthetic analogues of the natural product 1-O-acetylbritannilactone (ABL), a sesquiterpene isolated from the medicinal plant Inula britannica, have been prepared and exhibited significant in vitro cytotoxic activities against four cell lines including three human cancer cell lines (HCT116, HEp-2 and HeLa) and one normal hamster cell line (CHO). Structure-activity relationships indicate that esterification of 6-OH (enhanced lipophilicity) and α-methylene-γ-lactone functionalities play important roles in conferring cytotoxicity. Among the tested compounds, 14 bearing a lauroyl group (12C) at the 6-OH position displayed most potent in vitro cytotoxic activity, with IC50 values between 2.91 and 6.78 μM, comparable to the positive control etoposide (VP-16, IC50 values between 2.13 and 4.79 μM). Moreover, the compound 14 triggered remarkable apoptosis at a low concentration, and induced cell cycle arrest in G2/M phase in HCT116 cells. The biological assays conducted with normal cells (CHO) revealed that all the synthetic compounds are no selective against cancer cell lines tested. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  7. AaPDR3, a PDR Transporter 3, Is Involved in Sesquiterpene β-Caryophyllene Transport in Artemisia annua

    Science.gov (United States)

    Fu, Xueqing; Shi, Pu; He, Qian; Shen, Qian; Tang, Yueli; Pan, Qifang; Ma, Yanan; Yan, Tingxiang; Chen, Minghui; Hao, Xiaolong; Liu, Pin; Li, Ling; Wang, Yuliang; Sun, Xiaofen; Tang, Kexuan

    2017-01-01

    Artemisinin, a sesquiterpenoid endoperoxide, isolated from the plant Artemisia annua L., is widely used in the treatment of malaria. Another sesquiterpenoid, β-caryophyllene having antibiotic, antioxidant, anticarcinogenic and local anesthetic activities, is also presented in A. annua. The role played by sesquiterpene transporters in trichomes and accumulation of these metabolites is poorly understood in A. annua and in trichomes of other plant species. We identified AaPDR3, encoding a pleiotropic drug resistance (PDR) transporter located to the plasma membrane from A. annua. Expression of AaPDR3 is tissue-specifically and developmentally regulated in A. annua. GUS activity is primarily restricted to T-shaped trichomes of old leaves and roots of transgenic A. annua plants expressing proAaPDR3: GUS. The level of β-caryophyllene was decreased in transgenic A. annua plants expressing AaPDR3-RNAi while transgenic A. annua plants expressing increased levels of AaPDR3 accumulated higher levels of β-caryophyllene. When AaPDR3 was expressed in transformed yeast, yeasts expressing AaPDR3 accumulated more β-caryophyllene, rather than germacrene D and β-farnesene, compared to the non-expressing control. PMID:28533790

  8. Enzymatic synthesis of valerena-4,7(11)-diene by a unique sesquiterpene synthase from the valerian plant (Valeriana officinalis).

    Science.gov (United States)

    Pyle, Bryan W; Tran, Hue T; Pickel, Benjamin; Haslam, Tegan M; Gao, Zhizeng; MacNevin, Gillian; Vederas, John C; Kim, Soo-Un; Ro, Dae-Kyun

    2012-09-01

    Valerian (Valeriana officinalis) is a popular medicinal plant in North America and Europe. Its root extract is commonly used as a mild sedative and anxiolytic. Among dozens of chemical constituents (e.g. alkaloids, iridoids, flavonoids, and terpenoids) found in valerian root, valerena-4,7(11)-diene and valerenic acid (C15 sesquiterpenoid) have been suggested as the active ingredients responsible for the sedative effect. However, the biosynthesis of the valerena-4,7(11)-diene hydrocarbon skeleton in valerian remains unknown to date. To identify the responsible terpene synthase, next-generation sequencing (Roche 454 pyrosequencing) was used to generate ∼ 1 million transcript reads from valerian root. From the assembled transcripts, two sesquiterpene synthases were identified (VoTPS1 and VoTPS2), both of which showed predominant expression patterns in root. Transgenic yeast expressing VoTPS1 and VoTPS2 produced germacrene C/germacrene D and valerena-4,7(11)-diene, respectively, as major terpene products. Purified VoTPS1 and VoTPS2 recombinant enzymes confirmed these activities in vitro, with competent kinetic properties (K(m) of ∼ 10 μm and k(cat) of 0.01 s(-1) for both enzymes). The structure of the valerena-4,7(11)-diene produced from the yeast expressing VoTPS2 was further substantiated by (13) C-NMR and GC-MS in comparison with the synthetic standard. This study demonstrates an integrative approach involving next-generation sequencing and metabolically engineered microbes to expand our knowledge of terpenoid diversity in medicinal plants. © 2012 The Authors Journal compilation © 2012 FEBS.

  9. NF-κB p65 repression by the sesquiterpene lactone, Helenalin, contributes to the induction of autophagy cell death

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    Lim Chuan

    2012-07-01

    Full Text Available Abstract Background Numerous studies have demonstrated that autophagy plays a vital role in maintaining cellular homeostasis. Interestingly, several anticancer agents were found to exert their anticancer effects by triggering autophagy. Emerging data suggest that autophagy represents a novel mechanism that can be exploited for therapeutic benefit. Pharmacologically active natural compounds such as those from marine, terrestrial plants and animals represent a promising resource for novel anticancer drugs. There are several prominent examples from the past proving the success of natural products and derivatives exhibiting anticancer activity. Helenalin, a sesquiterpene lactone has been demonstrated to have potent anti-inflammatory and antitumor activity. Albeit previous studies demonstrating helenalin’s multi modal action on cellular proliferative and apoptosis, the mechanisms underlying its action are largely unexplained. Methods To deduce the mechanistic action of helenalin, cancer cells were treated with the drug at various concentrations and time intervals. Using western blot, FACS analysis, overexpression and knockdown studies, cellular signaling pathways were interrogated focusing on apoptosis and autophagy markers. Results We show here that helenalin induces sub-G1 arrest, apoptosis, caspase cleavage and increases the levels of the autophagic markers. Suppression of caspase cleavage by the pan caspase inhibitor, Z-VAD-fmk, suppressed induction of LC3-B and Atg12 and reduced autophagic cell death, indicating caspase activity was essential for autophagic cell death induced by helenalin. Additionally, helenalin suppressed NF-κB p65 expression in a dose and time dependent manner. Exogenous overexpression of p65 was accompanied by reduced levels of cell death whereas siRNA mediated suppression led to augmented levels of caspase cleavage, autophagic cell death markers and increased cell death. Conclusions Taken together, these results show

  10. trans-Caryophyllene, a Natural Sesquiterpene, Causes Tracheal Smooth Muscle Relaxation through Blockade of Voltage-Dependent Ca2+ Channels

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    Jader Santos Cruz

    2012-10-01

    Full Text Available trans-Caryophyllene is a major component in the essential oils of various species of medicinal plants used in popular medicine in Brazil. It belongs to the chemical class of the sesquiterpenes and has been the subject of a number of studies. Here, we evaluated the effects of this compound in airway smooth muscle. The biological activities of trans-caryophyllene were examined in isolated bath organs to investigate the effect in basal tonus. Electromechanical and pharmacomechanical couplings were evaluated through the responses to K+ depolarization and exposure to acetylcholine (ACh, respectively. Isolated cells of rat tracheal smooth muscle were used to investigate trans-caryophyllene effects on voltage-dependent Ca2+ channels by using the whole-cell voltage-clamp configuration of the patch-clamp technique. trans-Caryophyllene showed more efficiency in the blockade of electromechanical excitation-contraction coupling while it has only minor inhibitory effect on pharmacomechanical coupling. Epithelium removal does not modify tracheal smooth muscle response elicited by trans-caryophyllene in the pharmacomechanical coupling. Under Ca2+-free conditions, pre-exposure to trans-caryophyllene did not reduce the contraction induced by ACh in isolated rat tracheal smooth muscle, regardless of the presence of intact epithelium. In the whole-cell configuration, trans-caryophyllene (3 mM, inhibited the inward Ba2+ current (IBa to approximately 50% of control levels. Altogether, our results demonstrate that trans-caryophyllene has anti-spasmodic activity on rat tracheal smooth muscle which could be explained, at least in part, by the voltage-dependent Ca2+ channels blockade.

  11. Preventive and therapeutic anti-inflammatory properties of the sesquiterpene alpha-humulene in experimental airways allergic inflammation.

    Science.gov (United States)

    Rogerio, Alexandre P; Andrade, Edinéia L; Leite, Daniela F P; Figueiredo, Cláudia P; Calixto, João B

    2009-10-01

    alpha-Humulene and trans-caryophyllene are plant sesquiterpenes with pronounced anti-inflammatory properties. Here, we evaluated the effects of these compounds in an experimental model of airways allergic inflammation. Female BALB/c mice, sensitized to and challenged with ovalbumin received daily alpha-humulene or trans-caryophyllene (50 mg.kg(-1), orally) or alpha-humulene (1 mg.mL(-1), by aerosol) as either a preventive (for 22 days) or therapeutic (from the 18th to the 22nd day) treatment. Dexamethasone or budesonide was used as a positive control drug. Inflammation was determined on day 22 post-immunization by leukocyte recruitment, interleukin-5 (IL-5), CCL11, interferon-gamma (IFN-gamma) and leukotriene (LT)B(4) levels in bronchoalveolar lavage fluid (BALF). In addition, transcription factors [nuclear factor kappaB (NF-kappaB), activator protein 1 (AP-1)] and P-selectin in lung tissue were measured by immunohistochemistry and mucus secretion by histochemistry. Preventive or therapeutic treatments with alpha-humulene, but not with trans-caryophyllene, significantly reduced the eosinophil recruitment to the BALF. In addition, alpha-humulene recovery INF-gamma and reduced the IL-5, CCL11 and LTB(4) levels in BALF, as well as the IL-5 production in mediastinal lymph nodes (in vitro assay). Furthermore, alpha-humulene decreased the NF-kB and the AP-1 activation, the expression of P-selectin and the increased mucus secretion in the lung. alpha-Humulene, given either orally or by aerosol, exhibited marked anti-inflammatory properties in a murine model of airways allergic inflammation, an effect that seemed to be mediated via reduction of inflammatory mediators, adhesion molecule expression and transcription factors activation.

  12. The sesquiterpene lactone eupatolide sensitizes breast cancer cells to TRAIL through down-regulation of c-FLIP expression.

    Science.gov (United States)

    Lee, Jongkyu; Hwangbo, Cheol; Lee, Jung Joon; Seo, Juhee; Lee, Jeong-Hyung

    2010-01-01

    Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising candidate for cancer therapeutics due to its ability to induce apoptosis selectively in cancer cells. However, sensitivity of cancer cells for induction of apoptosis by TRAIL varies considerably. Therefore, it is important to develop agents that overcome this resistance. We show, for the first time, that eupatolide, the sesquiterpene lactone isolated from the medicinal plant Inula britannica, sensitizes human breast cancer cells to TRAIL-induced apoptosis. Treatment with TRAIL in combination with subtoxic concentrations of eupatolide enhanced the TRAIL-induced cytotoxicity in MCF-7, MDA-MB-231 and MDA-MB-453 breast cancer cells, whereas each reagent alone slightly induced cell death. The combination induced sub-G1 phase DNA content and annexin V-staining in MCF-7 cells, which are major features of apoptosis. Apoptotic characteristics induced by the combined treatment were significantly inhibited by a pan-caspase inhibitor. The sensitization to TRAIL-induced apoptosis was accompanied by the activation of caspase-8 and was concomitant with Bid and poly(ADP-ribose) polymerase (PARP) cleavage. Treatment of eupatolide alone significantly down-regulated the expression of cellular FLICE inhibitory protein (c-FLIP) in MCF-7 cells. Furthermore, enforced expression of c-FLIP significantly attenuated the apoptosis induced by this combination in MCF-7 cells, suggesting a key role for c-FLIP down-regulation in these events. We also observed that euaptolide inhibited AKT phosphorylation in a dose- and time-dependent manner. Moreover, inhibition of Akt by LY294002, a specific PI3K inhibitor, down-regulated c-FLIP expression in MCF-7 cells. Taken together, these results indicate that eupatolide could augment TRAIL-induced apoptosis in human breast cancer cells by down-regulating c-FLIP expression through the inhibition of AKT phosphorylation and be a valuable compound to overcome TRAIL resistance in

  13. Self-organizing maps of molecular descriptors for sesquiterpene lactones and their application to the chemotaxonomy of the Asteraceae family.

    Science.gov (United States)

    Scotti, Marcus T; Emerenciano, Vicente; Ferreira, Marcelo J P; Scotti, Luciana; Stefani, Ricardo; da Silva, Marcelo S; Mendonça Junior, Francisco Jaime B

    2012-04-20

    The Asteraceae, one of the largest families among angiosperms, is chemically characterised by the production of sesquiterpene lactones (SLs). A total of 1,111 SLs, which were extracted from 658 species, 161 genera, 63 subtribes and 15 tribes of Asteraceae, were represented and registered in two dimensions in the SISTEMATX, an in-house software system, and were associated with their botanical sources. The respective 11 block of descriptors: Constitutional, Functional groups, BCUT, Atom-centred, 2D autocorrelations, Topological, Geometrical, RDF, 3D-MoRSE, GETAWAY and WHIM were used as input data to separate the botanical occurrences through self-organising maps. Maps that were generated with each descriptor divided the Asteraceae tribes, with total index values between 66.7% and 83.6%. The analysis of the results shows evident similarities among the Heliantheae, Helenieae and Eupatorieae tribes as well as between the Anthemideae and Inuleae tribes. Those observations are in agreement with systematic classifications that were proposed by Bremer, which use mainly morphological and molecular data, therefore chemical markers partially corroborate with these classifications. The results demonstrate that the atom-centred and RDF descriptors can be used as a tool for taxonomic classification in low hierarchical levels, such as tribes. Descriptors obtained through fragments or by the two-dimensional representation of the SL structures were sufficient to obtain significant results, and better results were not achieved by using descriptors derived from three-dimensional representations of SLs. Such models based on physico-chemical properties can project new design SLs, similar structures from literature or even unreported structures in two-dimensional chemical space. Therefore, the generated SOMs can predict the most probable tribe where a biologically active molecule can be found according Bremer classification.

  14. Bioactivity of the extracts and isolation of lignans and a sesquiterpene from the aerial parts of Centaurea pamphylica (Asteraceae

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    Shoeb M.

    2007-06-01

    Full Text Available Centaurea pamphylica Boiss. & Heldr. (Family: Asteraceae, commonly known as ‘pamphylia daisy', is a Turkish endemic species of the genus Centaurea that comprises ca. 500 species, many of which have been used as traditional medicines.  The n-hexane, dichloromethane (DCM and methanol (MeOH extracts of the aerial parts of C. pamphylica were assessed for antioxidant activity and general toxicity using, respectively, the 2,2-diphenyl-1-picrylhydrazyl (DPPH, and the brine shrimp lethality assays. The reversed-phase preparative HPLC and PTLC were used to isolate compounds from the extracts.  The structures of these compounds [1-4] were elucidated by spectroscopic means, and also by direct comparison with the respective published data.  Both the DCM and the MeOH extract showed significant levels of antioxidant activities with the RC50 values of 72.6 x 10-2 and 47.3 ´ 10-2 mg/mL, respectively. The MeOH extract exhibited low levels of toxicity towards brine shrimps (LD50 = 125.0 ´ 10-2 mg/mL. Three major bioactive components of the MeOH extract were matairesinoside [1], arctiin [2] and matairesinol [3]. An eudesmane-type sesquiterpene, pterodontriol [4], was also isolated from the DCM extract.  Since reactive oxygen species are important contributors to various ailments, the antioxidant properties of the extracts as well as the isolated compounds may be of medicinal significance. This is the first report on the occurrence of 1-4 in C. pamphylica, and 4 in the genus Centaurea.  

  15. Norisoprenoids, sesquiterpenes and terpenoids content of Valpolicella wines during ageing: investigating aroma potential in relationship to evolution of tobacco and balsamic aroma in aged wine

    Science.gov (United States)

    Slaghenaufi, Davide; Ugliano, Maurizio

    2018-03-01

    During wine ageing, tobacco and balsamic aroma notes appear. In this paper, volatile compounds directly or potentially related to those aromas have been investigated in Corvina and Corvinone wines during aging. Corvina and Corvinone are two northern-Italy autochthonous red grape varieties, used to produce Valpolicella Classico and Amarone wines, both characterized by tobacco and balsamic aroma notes. Wines were analysed shortly after bottling or following model ageing at 60 °C for 48, 72, and 168 hours. Volatile compounds were analysed by HS-SPME-GC-MS. Results showed that compounds related to tobacco aroma (β-damascenone, 3-oxo-α-ionol, (E)-1-(2,3,6-Trimethylphenyl)-buta-1,3-diene (TPB) and megastigmatrienones) increased in relationship to storage time with different patterns. β-Damascenone and 3-oxo-α-ionol rapidly increased to reach a plateau in the first 48-72 hours of model ageing. Instead, TPB and megastigmatrienones concentration showed a linear correlation with ageing time. During model ageing, several cyclic terpenes tended to increase. Among them 1,8-cineole and 1,4-cineole, previously reported to contribute to red wine eucalyptus notes increased proportionally to storage time, and this behavior was clearly associated with reactions involving α-terpineol, limonene and terpinolene, as confirmed by studies with model wine solutions. Among other relevant volatile compounds, sesquiterpenes appear to contribute potentially balsamic and spicy aroma notes. In this study, linear sesquiterpenes (nerolidol, farnesol) underwent acid hydrolysis during long wine ageing, while cyclic sesquiterpenes seemed to increase with time. The chemical pathways associated with evolution of some of the compounds investigated have been studied in model wine.

  16. Norisoprenoids, Sesquiterpenes and Terpenoids Content of Valpolicella Wines During Aging: Investigating Aroma Potential in Relationship to Evolution of Tobacco and Balsamic Aroma in Aged Wine.

    Science.gov (United States)

    Slaghenaufi, Davide; Ugliano, Maurizio

    2018-01-01

    During wine aging, tobacco and balsamic aroma notes appear. In this paper, volatile compounds directly or potentially related to those aromas have been investigated in Corvina and Corvinone wines during aging. Corvina and Corvinone are two northern-Italy autochthonous red grape varieties, used to produce Valpolicella Classico and Amarone wines, both characterized by tobacco and balsamic aroma notes. Wines were analyzed shortly after bottling or following model aging at 60°C for 48, 72, and 168 h. Volatile compounds were analyzed by HS-SPME-GC-MS. Results showed that compounds related to tobacco aroma [β-damascenone, 3-oxo-α-ionol, (E)-1-(2,3,6-Trimethylphenyl)-buta-1,3-diene (TPB), and megastigmatrienones] increased in relationship to storage time with different patterns. β-Damascenone and 3-oxo-α-ionol rapidly increased to reach a plateau in the first 48-72 h of model aging. Instead, TPB and megastigmatrienones concentration showed a linear correlation with aging time. During model aging, several cyclic terpenes tended to increase. Among them 1,8-cineole and 1,4-cineole, previously reported to contribute to red wine eucalyptus notes increased proportionally to storage time, and this behavior was clearly associated with reactions involving α-terpineol, limonene, and terpinolene, as confirmed by studies with model wine solutions. Among other relevant volatile compounds, sesquiterpenes appear to contribute potentially balsamic and spicy aroma notes. In this study, linear sesquiterpenes (nerolidol, farnesol) underwent acid hydrolysis during long wine aging, while cyclic sesquiterpenes seemed to increase with time. The chemical pathways associated with evolution of some of the compounds investigated have been studied in model wine.

  17. Norisoprenoids, Sesquiterpenes and Terpenoids Content of Valpolicella Wines During Aging: Investigating Aroma Potential in Relationship to Evolution of Tobacco and Balsamic Aroma in Aged Wine

    Science.gov (United States)

    Slaghenaufi, Davide; Ugliano, Maurizio

    2018-01-01

    During wine aging, tobacco and balsamic aroma notes appear. In this paper, volatile compounds directly or potentially related to those aromas have been investigated in Corvina and Corvinone wines during aging. Corvina and Corvinone are two northern-Italy autochthonous red grape varieties, used to produce Valpolicella Classico and Amarone wines, both characterized by tobacco and balsamic aroma notes. Wines were analyzed shortly after bottling or following model aging at 60°C for 48, 72, and 168 h. Volatile compounds were analyzed by HS-SPME-GC-MS. Results showed that compounds related to tobacco aroma [β-damascenone, 3-oxo-α-ionol, (E)-1-(2,3,6-Trimethylphenyl)-buta-1,3-diene (TPB), and megastigmatrienones] increased in relationship to storage time with different patterns. β-Damascenone and 3-oxo-α-ionol rapidly increased to reach a plateau in the first 48–72 h of model aging. Instead, TPB and megastigmatrienones concentration showed a linear correlation with aging time. During model aging, several cyclic terpenes tended to increase. Among them 1,8-cineole and 1,4-cineole, previously reported to contribute to red wine eucalyptus notes increased proportionally to storage time, and this behavior was clearly associated with reactions involving α-terpineol, limonene, and terpinolene, as confirmed by studies with model wine solutions. Among other relevant volatile compounds, sesquiterpenes appear to contribute potentially balsamic and spicy aroma notes. In this study, linear sesquiterpenes (nerolidol, farnesol) underwent acid hydrolysis during long wine aging, while cyclic sesquiterpenes seemed to increase with time. The chemical pathways associated with evolution of some of the compounds investigated have been studied in model wine. PMID:29616214

  18. Norisoprenoids, Sesquiterpenes and Terpenoids Content of Valpolicella Wines During Aging: Investigating Aroma Potential in Relationship to Evolution of Tobacco and Balsamic Aroma in Aged Wine

    Directory of Open Access Journals (Sweden)

    Davide Slaghenaufi

    2018-03-01

    Full Text Available During wine aging, tobacco and balsamic aroma notes appear. In this paper, volatile compounds directly or potentially related to those aromas have been investigated in Corvina and Corvinone wines during aging. Corvina and Corvinone are two northern-Italy autochthonous red grape varieties, used to produce Valpolicella Classico and Amarone wines, both characterized by tobacco and balsamic aroma notes. Wines were analyzed shortly after bottling or following model aging at 60°C for 48, 72, and 168 h. Volatile compounds were analyzed by HS-SPME-GC-MS. Results showed that compounds related to tobacco aroma [β-damascenone, 3-oxo-α-ionol, (E-1-(2,3,6-Trimethylphenyl-buta-1,3-diene (TPB, and megastigmatrienones] increased in relationship to storage time with different patterns. β-Damascenone and 3-oxo-α-ionol rapidly increased to reach a plateau in the first 48–72 h of model aging. Instead, TPB and megastigmatrienones concentration showed a linear correlation with aging time. During model aging, several cyclic terpenes tended to increase. Among them 1,8-cineole and 1,4-cineole, previously reported to contribute to red wine eucalyptus notes increased proportionally to storage time, and this behavior was clearly associated with reactions involving α-terpineol, limonene, and terpinolene, as confirmed by studies with model wine solutions. Among other relevant volatile compounds, sesquiterpenes appear to contribute potentially balsamic and spicy aroma notes. In this study, linear sesquiterpenes (nerolidol, farnesol underwent acid hydrolysis during long wine aging, while cyclic sesquiterpenes seemed to increase with time. The chemical pathways associated with evolution of some of the compounds investigated have been studied in model wine.

  19. Sesquiterpenes produced by endophytic fungus Phomopsis cassiae with antifungal and acetylcholinesterase inhibition activities; Sesquiterpenos produzidos pelo fungo endofitico Phomopsis cassiae com atividade antifungica e inibidora de acetilcolinesterase

    Energy Technology Data Exchange (ETDEWEB)

    Zanardi, Lisineia M.; Bolzani, Vanderlan da S.; Cavalheiro, Alberto J.; Silva, Dulce H. Siqueira; Trevisan, Henrique C.; Araujo, Angela R. [UNESP, Araraquara, SP (Brazil). Inst. de Quimica; Silva, Geraldo H. [Universidade Federal de Sergipe (UFS), Aracaju, SE (Brazil). Centro de Ciencias Exatas e Tecnologia; Teles, Helder L. [Universidade Federal do Mato Grosso (UFMT), Rondonopolis, MT (Brazil). Dept. de Ciencias Biologicas; Young, Maria Claudia M., E-mail: araujoar@iq.unesp.br [Instituto de Botanica, Sao Paulo, SP (Brazil). Seccao de Fisiologia e Bioquimica de Plantas

    2012-07-01

    Two new diastereoisomeric cadinanes sesquiterpenes 3,9-dihydroxycalamenene (1-2), along with the known 3-hydroxycalamen-8-one (3) and aristelegone-A (4), were isolated from ethyl acetate extract of Phomopsis cassiae, an endophytic fungus in Cassia spectabilis. Their structures, including relative stereochemistry, were determined on the basis of detailed interpretation of 2D NMR spectra and comparison with related known compounds. Compounds 1-4 displayed antifungal activity against the phytopathogenic fungi Cladosporium cladosporioides and C. sphaerospermum, as well as inhibition of acetylcholinesterase. (author)

  20. Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus) show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi

    DEFF Research Database (Denmark)

    Pena-Espinoza, Miguel Angel; Boas, Ulrik; Williams, Andrew

    2015-01-01

    The study investigated direct anthelmintic effects of sesquiterpene lactones (SL)-containing extracts from forage chicory against free-living and parasitic stages of Ostertagia ostertagi. Freeze-dried leaves from chicory cultivars ‘Spadona’ and ‘Puna II’ were extracted using methanol/water. Total...... in worms exposed to any of the extracts. We have demonstrated that SL-containing extracts from forage chicory can inhibit feeding of free-living larvae and exert direct effects against parasitic stages of O. ostertagi. Our results may contribute to the identification of natural anti-parasitic compounds...

  1. ESTUDOS ESTILÍSTICOS NO BRASIL

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Falcão Uchôa

    2013-06-01

    Full Text Available Este artigo objetiva mostrar os inícios e a progressão dos estudos estilísticos no Brasil. Após uma introdução sobre o surgimento, na Europa, da Estilística como disciplina de pesquisa no campo da linguagem, detém-se, sucessivamente, nas contribuições de filólogos, linguistas, teóricos da literatura e gramáticos para o estudo dos mais variados recursos estilísticos ocorrentes sobretudo em autores brasileiros.

  2. O desamparo aprendido revisitado: estudos com animais

    Directory of Open Access Journals (Sweden)

    Maria Helena Leite Hunziker

    Full Text Available O desamparo aprendido tem sido definido como a dificuldade de aprendizagem apresentada por indivíduos que tiveram experiência prévia com estímulos aversivos incontroláveis. O objetivo deste trabalho é fazer uma revisão crítica dos estudos sobre o desamparo aprendido, com animais. Nessa análise, são considerados aspectos conceituais e metodológicos dos estudos em questão e as interpretações teóricas sobre esse efeito comportamental. Aborda-se a evolução histórica desses estudos, bem como alguns aspectos controversos das publicações que se acumularam ao longo de quatro décadas de pesquisa. A associação do desamparo aprendido com a depressão clínica é analisada criticamente, destacando-se a necessidade de maior rigor metodológico e conceitual nos estudos da área.

  3. Isolation of three sesquiterpene lactones from the roots of Cichorium glandulosum Boiss. et Huet. by high-speed counter-current chromatography.

    Science.gov (United States)

    Wu, Hankui; Su, Zhen; Yang, Yi; Ba, Hang; Aisa, Haji Akber

    2007-12-28

    Because of the skeletal complexity and similarity of the polarity, little research was reported on the isolation of sesquiterpene lactones by high-speed counter-current chromatography (HSCCC). Herein, three sesquiterpene lactones were successfully purified from the ethyl acetate extract of the roots of the traditional Uyghur medicinal plant Cichorium glandulosum Boiss. et Huet. by HSCCC. The separation was performed in two steps with two solvent systems: n-hexane-ethyl acetate-methanol-water (1.5:5:2.75:5, v/v/v/v) and ethyl acetate-methanol-water (20:1:20, v/v/v). From 166 mg of the ethyl acetate extract, 19 mg of lactucopicrin was isolated with the first solvent system and 10 mg of 11beta,13-dihydrolactucin and 16 mg of lactucin were obtained with the second solvent system. All purified compounds were over 94% purity as determined by HPLC analysis, and these chemical structures were confirmed by (1)H NMR and (13)C NMR.

  4. The bouquet of grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) flowers arises from the biosynthesis of sesquiterpene volatiles in pollen grains

    Science.gov (United States)

    Martin, Diane M.; Toub, Omid; Chiang, Angela; Lo, Bernard C.; Ohse, Sebastian; Lund, Steven T.; Bohlmann, Jörg

    2009-01-01

    Terpenoid volatiles are important information molecules that enable pollinators to locate flowers and may protect reproductive tissues against pathogens or herbivores. Inflorescences of grapevine (Vitis vinifera L.) are composed of tiny green flowers that produce an abundance of sesquiterpenoid volatiles. We demonstrate that male flower parts of grapevines are responsible for sesquiterpenoid floral scent formation. We describe temporal and spatial patterns of biosynthesis and release of floral volatiles throughout the blooming of V. vinifera L. cv. Cabernet Sauvignon. The biosynthesis of sesquiterpene volatiles, which are emitted with a light-dependent diurnal pattern early in the morning at prebloom and bloom, is localized to anthers and, more specifically, within the developing pollen grains. Valencene synthase (VvValCS) enzyme activity, which produces the major sesquiterpene volatiles of grapevine flowers, is present in anthers. VvValCS transcripts are most abundant in flowers at prebloom stages. Western blot analysis identified VvValCS protein in anthers, and in situ immunolabeling located VvValCS protein in pollen grains during bloom. Histochemical staining, as well as immunolabeling analysis by fluorescent microscopy and transmission electron microscopy, indicated that VvValCS localizes close to lipid bodies within the maturing microspore. PMID:19359488

  5. The mono - and sesquiterpene content of aphid-induced galls on Pistacia palaestina is not a simple reflection of their composition in intact leaves.

    Science.gov (United States)

    Rand, Karin; Bar, Einat; Ben-Ari, Matan; Lewinsohn, Efraim; Inbar, Moshe

    2014-06-01

    Pistacia palaestina Boiss. (Anacardiaceae), a sibling species of P. terebinthus also known as turpentine tree or terebinth tree, is common in the Levant region. The aphid Baizongia pistaciae L. manipulates the leaves of the plant to form large galls, which provide both food and protection for its developing offspring. We analyzed the levels and composition of mono-and sesquiterpenes in both leaves and galls of ten naturally growing trees. Our results show that monoterpene hydrocarbons are the main constituents of P. palaestina leaves and galls, but terpene levels and composition vary among trees. Despite this inter-tree variation, terpene levels and compositions in galls from different trees resemble each other more than the patterns displayed by leaves from the same trees. Generally, galls contain 10 to 60 fold higher total terpene amounts than leaves, especially of the monoterpenes α-pinene and limonene. Conversely, the leaves generally accumulate more sesquiterpenes, in particular E-caryophyllene, germacrene D and δ-cadinene, in comparison to galls. Our results clearly show that the terpene pattern in the galls is not a simple reflection of that of the leaves and suggest that aphids have a strong impact on the metabolism of their host plant, possibly for their own defense.

  6. New sesquiterpene dimers from Inula britannica inhibit NF-kappaB activation and NO and TNF-alpha production in LPS-stimulated RAW264.7 cells.

    Science.gov (United States)

    Jin, Hui Zi; Lee, Dongho; Lee, Jeong Hyung; Lee, Kyeong; Hong, Young-Soo; Choung, Dong-Ho; Kim, Young Ho; Lee, Jung Joon

    2006-01-01

    A bioassay-guided isolation of an ethyl acetate-soluble extract of the aerial parts of Inula britannica var. chinensis (Rupr.) Regel, using an in vitro NF-kappaB reporter gene assay, led to the isolation of four new sesquiterpene dimers bearing a norbornene moiety, inulanolides A-D, and three known sesquiterpenes, 1,6alpha-dihydroxyeriolanolide, 1-acetoxy-6alpha-hydroxyeriolanolide, and eupatolide. The structures of the new compounds were elucidated by spectroscopic methods. Among these compounds, inulanolides B and D and eupatolide, exhibited potent inhibitory activity on the LPS-induced NF-kappaB activation with IC50 values of 0.49 microM, 0.48 microM, and 1.54 microM, respectively. Consistent with their inhibitory effect on NF-kappaB activation, compounds and also strongly inhibited the production of NO and TNF-alpha in the LPS-stimulated RAW264.7 cells with IC50 values in the range of 2 microM.

  7. In vivo assessment of plant extracts for control of plant diseases: A sesquiterpene ketolactone isolated from Curcuma zedoaria suppresses wheat leaf rust.

    Science.gov (United States)

    Han, Jae Woo; Shim, Sang Hee; Jang, Kyoung Soo; Choi, Yong Ho; Dang, Quang Le; Kim, Hun; Choi, Gyung Ja

    2018-02-01

    As an alternative to synthetic pesticides, natural materials such as plant extracts and microbes have been considered to control plant diseases. In this study, methanol extracts of 120 plants were explored for in vivo antifungal activity against Rhizoctonia solani, Botrytis cinerea, Phytophthora infestans, Puccinia triticina, and Blumeria graminis f. sp. hordei. Of the 120 plant extracts, eight plant extracts exhibited a disease control efficacy of more than 90% against at least one of five plant diseases. In particular, a methanol extract of Curcuma zedoaria rhizomes exhibited strong activity against wheat leaf rust caused by P. triticina. When the C. zedoaria methanol extracts were partitioned with various solvents, the layers of n-hexane, methylene chloride, and ethyl acetate showed disease control values of 100, 80, and 43%, respectively, against wheat leaf rust. From the C. zedoaria rhizome extracts, an antifungal substance was isolated and identified as a sesquiterpene ketolactone based on the mass and nuclear magnetic resonance spectral data. The active compound controlled the development of rice sheath blight, wheat leaf rust, and tomato late blight. Considering the in vivo antifungal activities of the sesquiterpene ketolactone and the C. zedoaria extracts, these results suggest that C. zedoaria can be used as a potent fungicide in organic agriculture.

  8. Activity studies of sesquiterpene oxides and sulfides from the plant Hyptis suaveolens (Lamiaceae) and its repellency on Ixodes ricinus (Acari: Ixodidae).

    Science.gov (United States)

    Ashitani, T; Garboui, S S; Schubert, F; Vongsombath, C; Liblikas, I; Pålsson, K; Borg-Karlson, A-K

    2015-12-01

    Hyptis suaveolens (Lamiaceae), a plant traditionally used as a mosquito repellent, has been investigated for repellent properties against nymphs of the tick Ixodes ricinus. Essential oils and volatile compounds of fresh and dried leaves, from plants originating from Laos and Guinea-Bissau, were identified by GC-MS and tested in a tick repellency bioassay. All the essential oils were strongly repellent against the ticks, even though the main volatile constituents differed in their proportions of potentially tick repellent chemicals. (+)/(-)-sabinene were present in high amounts in all preparations, and dominated the emission from dry and fresh leaves together with 1,8-cineol and α-phellandrene. 1,8-Cineol and sabinene were major compounds in the essential oils from H. suaveolens from Laos. Main compounds in H. suaveolens from Guinea-Bissau were (-)-sabinene, limonene and terpinolene. Among the sesquiterpene hydrocarbons identified, α-humulene exhibited strong tick repellency (96.8 %). Structure activity studies of oxidation or sulfidation products of germacrene D, α-humulene and β-caryophyllene, showed increased tick repellent activity: of mint sulfide (59.4 %), humulene-6,7-oxide (94.5 %) and caryophyllene-6,7-oxide (96.9 %). The substitution of oxygen with sulfur slightly lowered the repellency. The effects of the constituents in the oils can then be regarded as a trade off between the subsequently lower volatility of the sesquiterpene derivatives compared to the monoterpenes and may thus increase their potential usefulness as tick repellents.

  9. Biosynthesis of Costunolide, Dihydrocostunolide, and Leucodin. Demonstration of Cytochrome P450-Catalyzed Formation of the Lactone Ring Present in Sesquiterpene Lactones of Chicory

    Science.gov (United States)

    de Kraker, Jan-Willem; Franssen, Maurice C.R.; Joerink, Maaike; de Groot, Aede; Bouwmeester, Harro J.

    2002-01-01

    Chicory (Cichorium intybus) is known to contain guaianolides, eudesmanolides, and germacranolides. These sesquiterpene lactones are postulated to originate from a common germacranolide, namely (+)-costunolide. Whereas a pathway for the formation of germacra-1(10),4,11(13)-trien-12-oic acid from farnesyl diphosphate had previously been established, we now report the isolation of an enzyme activity from chicory roots that converts the germacrene acid into (+)-costunolide. This (+)-costunolide synthase catalyzes the last step in the formation of the lactone ring present in sesquiterpene lactones and is dependent on NADPH and molecular oxygen. Incubation of the germacrene acid in the presence of 18O2 resulted in the incorporation of one atom of 18O into (+)-costunolide. The label was situated at the ring oxygen atom. Hence, formation of the lactone ring most likely occurs via C6-hydroxylation of the germacrene acid and subsequent attack of this hydroxyl group at the C12-atom of the carboxyl group. Blue light-reversible CO inhibition and experiments with cytochrome P450 inhibitors demonstrated that the (+)-costunolide synthase is a cytochrome P450 enzyme. In addition, enzymatic conversion of (+)-costunolide into 11(S),13-dihydrocostunolide and leucodin, a guaianolide, was detected. The first-mentioned reaction involves an enoate reductase, whereas the formation of leucodin from (+)-costunolide probably involves more than one enzyme, including a cytochrome P450 enzyme. PMID:12011356

  10. Insights into the molecular mechanism of action of Celastraceae sesquiterpenes as specific, non-transported inhibitors of human P-glycoprotein.

    Science.gov (United States)

    Muñoz-Martínez, Francisco; Reyes, Carolina P; Pérez-Lomas, Antonio L; Jiménez, Ignacio A; Gamarro, Francisco; Castanys, Santiago

    2006-01-01

    Dihydro-beta-agarofuran sesquiterpenes from Celastraceae have been recently shown to bind to human P-glycoprotein (Pgp), functioning as specific, mixed-type inhibitors of its drug transport activity, as well as multidrug resistance (MDR) modulators in vitro. However, nothing is known about whether such compounds are themselves transported by Pgp, or whether they affect Pgp expression as well as its activity, or about the location of their binding site within the protein. We performed transport experiments with a newly synthesized fluorescent sesquiterpene derivative, which retains the anti-Pgp activity of its natural precursor. This probe was poorly transported by Pgp, MRP1, MRP2 and BCRP transporters, compared with classical MDR substrates. Moreover, Pgp did not confer cross-resistance to the most potent dihydro-beta-agarofurans, which did not affect Pgp expression levels in several MDR cell lines. Finally, we observed competitive and non-competitive interactions between one of such dihydro-beta-agarofurans (Mama12) and classical Pgp modulators such as cyclosporin A, verapamil, progesterone, vinblastine and GF120918. These findings suggest that multidrug ABC transporters do not confer resistance to dihydro-beta-agarofurans and could not affect their absorption and biodistribution in the body. Moreover, we mapped their binding site(s) within Pgp, which may prove useful for the rational design of improved modulators based on the structure of dihydro-beta-agarofurans.

  11. Sesquiterpene lactones isolated from indigenous Middle Eastern plants inhibit tumor promoter-induced transformation of JB6 cells

    Directory of Open Access Journals (Sweden)

    Saikali Melody

    2012-07-01

    Full Text Available Abstract Background Sesquiterpene lactones (SL are plant secondary metabolites that are known for their anti-fungal, anti-bacterial, anti-inflammatory, and anti-tumor properties. Considering that several SL-derived drugs are currently in cancer clinical trials, we have tested two SL molecules, 3-β-methoxy-iso-seco-tanapartholide (β-tan isolated from Achillea falcata and salograviolide A (Sal A isolated from Centaurea ainetensis, for their anti-tumor properties. We used the mouse epidermal JB6P + cells as a model for tumor promotion and cellular transformation. Key players that are involved in cellular transformation and tumorigenesis are the AP-1 and NF-κB transcription factors; therefore, we assessed how β-tan and Sal A modulate their signaling pathways in JB6P + cells. Methods The effects of β-tan and Sal A on the growth of normal and neoplastic keratinocytes and on the tumor promotion-responsive JB6P + cells were determined using the MTT assay. Anchorage-independent cell growth transformation assays were used to evaluate the anti-tumor promoting properties of these SL molecules in JB6P + cells and dual luciferase reporter assays and western blot analysis were used to investigate their effects on tumor promoter-induced AP-1 and NF-κB activities and protein levels of key AP-1 and NF-кB target genes. Results β-tan and Sal A selectively inhibited tumor promoter-induced cell growth and transformation of JB6P + cells at concentrations that do not affect JB6P + and primary keratinocytes basal cell growth. In addition, both molecules reduced basal and tumor promoter-induced NF-κB transcriptional activities, differentially regulated basal and tumor promoter-induced AP-1 transcriptional activities, and modulated key players of the AP-1 and NF-κB signaling pathways. Conclusions These results highlight the anti-tumor promoting properties of β-tan and Sal A. These SL molecules isolated from two plant species native to

  12. Sesquiterpene lactones isolated from indigenous Middle Eastern plants inhibit tumor promoter-induced transformation of JB6 cells.

    Science.gov (United States)

    Saikali, Melody; Ghantous, Akram; Halawi, Racha; Talhouk, Salma N; Saliba, Najat A; Darwiche, Nadine

    2012-07-09

    Sesquiterpene lactones (SL) are plant secondary metabolites that are known for their anti-fungal, anti-bacterial, anti-inflammatory, and anti-tumor properties. Considering that several SL-derived drugs are currently in cancer clinical trials, we have tested two SL molecules, 3-β-methoxy-iso-seco-tanapartholide (β-tan) isolated from Achillea falcata and salograviolide A (Sal A) isolated from Centaurea ainetensis, for their anti-tumor properties. We used the mouse epidermal JB6P + cells as a model for tumor promotion and cellular transformation. Key players that are involved in cellular transformation and tumorigenesis are the AP-1 and NF-κB transcription factors; therefore, we assessed how β-tan and Sal A modulate their signaling pathways in JB6P + cells. The effects of β-tan and Sal A on the growth of normal and neoplastic keratinocytes and on the tumor promotion-responsive JB6P + cells were determined using the MTT assay. Anchorage-independent cell growth transformation assays were used to evaluate the anti-tumor promoting properties of these SL molecules in JB6P + cells and dual luciferase reporter assays and western blot analysis were used to investigate their effects on tumor promoter-induced AP-1 and NF-κB activities and protein levels of key AP-1 and NF-кB target genes. β-tan and Sal A selectively inhibited tumor promoter-induced cell growth and transformation of JB6P + cells at concentrations that do not affect JB6P + and primary keratinocytes basal cell growth. In addition, both molecules reduced basal and tumor promoter-induced NF-κB transcriptional activities, differentially regulated basal and tumor promoter-induced AP-1 transcriptional activities, and modulated key players of the AP-1 and NF-κB signaling pathways. These results highlight the anti-tumor promoting properties of β-tan and Sal A. These SL molecules isolated from two plant species native to the Middle East may provide opportunities for complementary

  13. Preventive and therapeutic anti-inflammatory properties of the sesquiterpene α-humulene in experimental airways allergic inflammation

    Science.gov (United States)

    Rogerio, Alexandre P; Andrade, Edinéia L; Leite, Daniela FP; Figueiredo, Cláudia P; Calixto, João B

    2009-01-01

    Background and purpose: α-Humulene and trans-caryophyllene are plant sesquiterpenes with pronounced anti-inflammatory properties. Here, we evaluated the effects of these compounds in an experimental model of airways allergic inflammation. Experimental approach: Female BALB/c mice, sensitized to and challenged with ovalbumin received daily α-humulene or trans-caryophyllene (50 mg·kg−1, orally) or α-humulene (1 mg·mL−1, by aerosol) as either a preventive (for 22 days) or therapeutic (from the 18th to the 22nd day) treatment. Dexamethasone or budesonide was used as a positive control drug. Inflammation was determined on day 22 post-immunization by leukocyte recruitment, interleukin-5 (IL-5), CCL11, interferon-γ (IFN-γ) and leukotriene (LT)B4 levels in bronchoalveolar lavage fluid (BALF). In addition, transcription factors [nuclear factor κB (NF-κB), activator protein 1 (AP-1)] and P-selectin in lung tissue were measured by immunohistochemistry and mucus secretion by histochemistry. Key results: Preventive or therapeutic treatments with α-humulene, but not with trans-caryophyllene, significantly reduced the eosinophil recruitment to the BALF. In addition, α-humulene recovery INF-γ and reduced the IL-5, CCL11 and LTB4 levels in BALF, as well as the IL-5 production in mediastinal lymph nodes (in vitro assay). Furthermore, α-humulene decreased the NF-kB and the AP-1 activation, the expression of P-selectin and the increased mucus secretion in the lung. Conclusions and implications: α-Humulene, given either orally or by aerosol, exhibited marked anti-inflammatory properties in a murine model of airways allergic inflammation, an effect that seemed to be mediated via reduction of inflammatory mediators, adhesion molecule expression and transcription factors activation. This article is part of a themed issue on Mediators and Receptors in the Resolution of Inflammation. To view this issue visit http://www3.interscience.wiley.com/journal/121548564/issueyear

  14. SHORT COMMUNICATION CUAUTHEMONE SESQUITERPENES ...

    African Journals Online (AJOL)

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    chemotaxonomic relationship of the species within the genus Laggera, and between L. tomentosa and Pluchea species. EXPERIMENTAL. General. 1H, 13C, and 2D NMR spectra were recorded on a Bruker Avance 400MHz spectrometer with TMS as internal standard. The ultraviolet and visible (UV-Vis) spectra were.

  15. Effects of gamma irradiation and comparison of different extraction methods on sesquiterpene lactone yields from the medicinal plant Thapsia garganica L. (Apiaceae)

    DEFF Research Database (Denmark)

    Mohamed Ibrahim, Abir M.; Martinez-Swatson, Karen A.; Benkaci-Ali, Farid

    2017-01-01

    methods Quantification of the compounds of interest was done using an HPLC. The antioxidant activity extracts was determined using the two free radical scavenging assays: the 2,2-diphenyl-picryl-hydrazyl (DPPH) and the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS). Results......: It was found that location and extraction method had significant impact on the phytochemical composition of extracts. Gamma irradiation was found to have no effect on the phytochemical composition of the plant extracts or on their antioxidant properties. Conclusion: The study has shown that microwave assisted......, and with the direct application of root sections for the soothing of dental pains. Aim of the study: The objective of this study was to evaluate the combined effect of microwave assisted extraction and gamma irradiation on sesquiterpene lactones in T. garganica extracts. Materials and methods: To evaluate...

  16. The inflammatory and tumor-promoting sesquiterpene lactone, thapsigargin, activates platelets by selective mobilization of calcium as shown by protein phosphorylations

    DEFF Research Database (Denmark)

    Thastrup, Ole; Linnebjerg, H; Bjerrum, P J

    1987-01-01

    , raised cytoplasmic free calcium level and phosphorylation of platelet proteins was examined in platelet-rich plasma and washed platelet suspension. In contrast to A23187 and thrombin, the platelet activation induced by thapsigargin developed slowly, with maximal response obtained after 2-3 min. Both......We have studied the activation of human blood platelets by the inflammatory and tumor-promoting sesquiterpene lactone, thapsigargin. The effect of thapsigargin was compared with other common agonists (calcium ionophore A23187, phorbol ester TPA and thrombin). Platelet aggregation, serotonin release...... the thapsigargin- and the A23187-induced serotonin releases were synergistically increased by TPA. Studies of the phosphorylation of platelet proteins revealed that thapsigargin and A23187 equally well induced a selective phosphorylation of two proteins with apparent molecular masses of 20 kDa and 47 k...

  17. Shizukaol D, a Dimeric Sesquiterpene Isolated from Chloranthus serratus, Represses the Growth of Human Liver Cancer Cells by Modulating Wnt Signalling Pathway.

    Directory of Open Access Journals (Sweden)

    Lisha Tang

    Full Text Available Natural products have become sources of developing new drugs for the treatment of cancer. To seek candidate compounds that inhibit the growth of liver cancer, components of Chloranthus serratus were tested. Here, we report that shizukaol D, a dimeric sesquiterpene from Chloranthus serratus, exerted a growth inhibition effect on liver cancer cells in a dose- and time-dependent manner. We demonstrated that shizukaol D induced cells to undergo apoptosis. More importantly, shizukaol D attenuated Wnt signalling and reduced the expression of endogenous Wnt target genes, which resulted in decreased expression of β-catenin. Collectively, this study demonstrated that shizukaol D inhibited the growth of liver cancer cells by modulating Wnt pathway.

  18. A Sesquiterpene Quinone, 5-Epi-smenospongine, Promotes TNF-α Production in LPS-stimulated RAW 264.7 Cells

    Directory of Open Access Journals (Sweden)

    Masataka Mochizuki

    2007-10-01

    Full Text Available Eight sesquiterpene quinones: ilimaquinone (1, smenospongidine (3,smenospongiarine (5, smenospongine (7, and their corresponding 5-epimers 2, 4, 6, and 8,isolated from the Palauan marine sponge Hippospongia sp., were examined regarding theireffects on TNF-α production in LPS-stimulated RAW 264.7 cells. 5-Epi-smenospongine(8 promoted the production of TNF-α to a level three times greater than the control at10 μM, but compounds 1-7 did not show apparent activity. The results suggest that thecis-decaline ring and a primary amine in the benzoquinone ring are necessary for activity.This is the first study to report the modulation of TNF-α production by a sesquiterpenequinone.

  19. Theoretical and experimental analysis of the reaction mechanism of MrTPS2, a triquinane-forming sesquiterpene synthase from chamomile.

    Science.gov (United States)

    Hong, Young J; Irmisch, Sandra; Wang, Selina C; Garms, Stefan; Gershenzon, Jonathan; Zu, Liansuo; Köllner, Tobias G; Tantillo, Dean J

    2013-09-27

    Terpene synthases, as key enzymes of terpene biosynthesis, have garnered the attention of chemists and biologists for many years. Their carbocationic reaction mechanisms are responsible for the huge variety of terpene structures in nature. These mechanisms are amenable to study by using classical biochemical approaches as well as computational analysis, and in this study we combine quantum-chemical calculations and deuterium-labeling experiments to elucidate the reaction mechanism of a triquinane forming sesquiterpene synthase from chamomile. Our results suggest that the reaction from farnesyl diphosphate to triquinanes proceeds through caryophyllyl and presilphiperfolanyl cations and involves the protonation of a stable (-)-(E)-β-caryophyllene intermediate. A tyrosine residue was identified that appears to be involved in the proton-transfer process. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Two Novel Tyrosinase Inhibitory Sesquiterpenes Induced by CuCl2 from a Marine-Derived Fungus Pestalotiopsis sp. Z233

    Directory of Open Access Journals (Sweden)

    Min Sun

    2013-08-01

    Full Text Available Two new sesquiterpenes, 1β,5α,6α,14-tetraacetoxy-9α-benzoyloxy-7β H-eudesman-2β,11-diol (1 and 4α,5α-diacetoxy-9α-benzoyloxy-7βH-eudesman-1β,2β,11, 14-tetraol (2, were produced as stress metabolites in the cultured mycelia of Pestalotiopsis sp. Z233 isolated from the algae Sargassum horneri in response to abiotic stress elicitation by CuCl2. Their structures were established by spectroscopic means. New compounds 1 and 2 showed tyrosinase inhibitory activities with IC50 value of 14.8 µM and 22.3 µM.

  1. Germacrane sesquiterpenes isolated from the rhizome of Curcuma xanthorrhiza Roxb. inhibit UVB-induced upregulation of MMP-1, -2, and -3 expression in human keratinocytes.

    Science.gov (United States)

    Park, Ji-Hae; Mohamed, Mohamed Antar Aziz; Jung, Ye-Jin; Shrestha, Sabina; Lee, Tae Hoon; Lee, Chang-Ho; Han, Daeseok; Kim, Jiyoung; Baek, Nam-In

    2015-10-01

    Four sesquiterpenes were isolated from the rhizome of Curcuma xanthorrhiza Roxb.: furanodiene (1), germacrone (2), furanodienone (3), and 13-hydroxygermacrone (4). Importantly, this was the first time compounds 1 and 4 were isolated from this plant. The chemical structures of these compounds were determined using 1D- and 2D-nuclear magnetic resonance, infrared spectroscopy, and electron ionization mass spectrometry analyses. Among the isolated compounds, compounds 2 and 4 inhibited UVB-induced upregulation of the mRNA and protein expression levels of MMP-1, MMP-2, and MMP-3 in human keratinocytes (HaCaT). Moreover, this upregulation occurred in a dose-dependent manner over the range of 1-10 μM for each compound.

  2. Acanthamoeba castellanii Neff: In vitro activity against the trophozoite stage of a natural sesquiterpene and a synthetic cobalt(II)-lapachol complex.

    Science.gov (United States)

    Martín-Navarro, Carmen M; López-Arencibia, Atteneri; Lorenzo-Morales, Jacob; Oramas-Royo, Sandra; Hernández-Molina, Rita; Estévez-Braun, Ana; Ravelo, Angel G; Valladares, Basilio; Piñero, José E

    2010-09-01

    In this study, the in vitro activities of a natural sesquiterpene, alpha-cyperotundone, isolated from the root bark of Maytenus retusa and a cobalt(II)-complex of a natural occurring prenyl hydroxynaphthoquinone (lapachol) were evaluated against the trophozoite stage of Acanthamoeba castellanii Neff using a previously developed colorimetric 96-well microtiter plate assay, based on the oxido-reduction of Alamar Blue(R). The obtained activities showed that these two compounds were able to inhibit the in vitro growth of the amoebae at relatively low concentrations. Further identification of the molecular targets of these products and their effects on acanthamoebae should be determined to evaluate their possible therapeutic use. Copyright 2010. Published by Elsevier Inc.

  3. Gerencia de Informacao em Estudos Multicentricos: o Estudo Longitudinal de Saude do Adulto

    OpenAIRE

    Bruce Bartholow Duncan; Alvaro Vigo; Emerson Hernandez; Vivian Cristine Luft; Hubert Ahlert; Kaiser Bergmann; Eduardo Mota

    2013-01-01

    A gerência da informação em estudos multicêntricos de grande porte requer uma abordagem especializada. O Estudo Longitudinal da Saúde do Adulto (ELSA-Brasil) criou um Centro de Dados para delinear e gerenciar seu sistema de dados. O objetivo do artigo foi descrever os passos envolvidos, incluindo os métodos de entrada, transmissão e gerência de informações. Foi desenvolvido um sistema web que permitiu, de forma segura e confidencial, a entrada online, verificação e edição, bem como incorporaç...

  4. The floral transcriptome of ylang ylang (Cananga odorata var. fruticosa) uncovers biosynthetic pathways for volatile organic compounds and a multifunctional and novel sesquiterpene synthase.

    Science.gov (United States)

    Jin, Jingjing; Kim, Mi Jung; Dhandapani, Savitha; Tjhang, Jessica Gambino; Yin, Jun-Lin; Wong, Limsoon; Sarojam, Rajani; Chua, Nam-Hai; Jang, In-Cheol

    2015-07-01

    The pleasant fragrance of ylang ylang varieties (Cananga odorata) is mainly due to volatile organic compounds (VOCs) produced by the flowers. Floral scents are a key factor in plant-insect interactions and are vital for successful pollination. C. odorata var. fruticosa, or dwarf ylang ylang, is a variety of ylang ylang that is popularly grown in Southeast Asia as a small shrub with aromatic flowers. Here, we describe the combined use of bioinformatics and chemical analysis to discover genes for the VOC biosynthesis pathways and related genes. The scented flowers of C. odorata var. fruticosa were analysed by gas chromatography/mass spectrometry and a total of 49 VOCs were identified at four different stages of flower development. The bulk of these VOCs were terpenes, mainly sesquiterpenes. To identify the various terpene synthases (TPSs) involved in the production of these essential oils, we performed RNA sequencing on mature flowers. From the RNA sequencing data, four full-length TPSs were functionally characterized. In vitro assays showed that two of these TPSs were mono-TPSs. CoTPS1 synthesized four products corresponding to β-thujene, sabinene, β-pinene, and α-terpinene from geranyl pyrophosphate and CoTPS4 produced geraniol from geranyl pyrophosphate. The other two TPSs were identified as sesqui-TPSs. CoTPS3 catalysed the conversion of farnesyl pyrophosphate to α-bergamotene, whereas CoTPS2 was found to be a multifunctional and novel TPS that could catalyse the synthesis of three sesquiterpenes, β-ylangene, β-copaene, and β-cubebene. Additionally, the activities of the two sesqui-TPSs were confirmed in planta by transient expression of these TPS genes in Nicotiana benthamiana leaves by Agrobacterium-mediated infiltration. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  5. Chemically Induced Cryptic Sesquiterpenoids and Expression of Sesquiterpene Cyclases in Botrytis cinerea Revealed New Sporogenic (+)-4-Epieremophil-9-en-11-ols.

    Science.gov (United States)

    Pinedo, Cristina; Moraga, Javier; Barua, Javier; González-Rodríguez, Victoria E; Aleu, Josefina; Durán-Patrón, Rosa; Macías-Sánchez, Antonio J; Hanson, James R; Viaud, Muriel; Hernández-Galán, Rosario; Garrido, Carlos; Collado, Isidro G

    2016-05-20

    The sequencing of the genomes of the B05.10 and T4 strains of the fungus Botrytis cinerea revealed an abundance of novel biosynthetic gene clusters, the majority of which were unexpected on the basis of the previous analyses of the fermentation of these and closely related species. By systematic alteration of easy accessible cultivation parameters, using chemical induction with copper sulfate, we have found a cryptic sesquiterpenoid family with new structures related to eremophil-9-ene, which had the basic structure of the sesquiterpene (+)-5-epiaristolochene ((+)-4-epieremophil-9-ene). An expression study of the sesquiterpene cyclase genes present in the Botrytis cinerea genome, under culture conditions, is reported. In general, a 3 day delay and a higher BcSTC genes expression were observed when copper (5 ppm) was fed to the fermentation broth. In addition, to the observed effect on the BcBOT2 (BcSTC1) gene, involved in the biosynthesis of the botrydial toxin, a higher expression level for BcSTC3 and BcSTC4 was observed with respect to the control in the strain B05.10. Interestingly, under copper conditions, the BcSTC4 gene was the most expressed gene in the Botrytis cinerea UCA992 strain. In vitro evaluation of the biological role of these metabolites indicates that they contributed to the conidial development in B. cinerea and appear to be involved in self-regulation of the production of asexual spores. Furthermore, they promoted the formation of complex appressoria or infection cushions.

  6. Evaluation of sesquiterpene coumarins from Ferula assa-foetida on VEGF, MMP9, MMP2 and study of biding modes using computational methods

    Directory of Open Access Journals (Sweden)

    Kh. Almasi

    2017-11-01

    Full Text Available Background and objectives: Ferula assafoetida of Apiaceae family bears sesquiterpene coumarins from phenolic class. Studies have shown that phenolic compounds at physiological concentration can inhibit two groups of gelatinase matrix metalloproteinases. So, the ability of compounds of this plant to inhibit the enzymes mentioned above seems to be useful. Methods: Acetone extract of plant was prepared and sesquiterpene coumarins were purified using column chromatography and HPLC preparative analyses and their structures were elucidated. After culturing the cell to proper confluence, the cells were isolated and the supernatant was removed. The pure substances were applied on cell lines U87MG and WEHI. In the computational part, the structure has been docked in the active site of metalloproteinase, and significant interactions were determined. Subsequently, ligand-protein complexes were subjected to molecular dynamics simulation in water and thermodynamic properties were calculated. Results: In the phytochemistry field galbanic acid, mogoltadone, kellerin, polyanthin and polyanthinin were extracted from F. assafoetida. Biological investigation demonstrated significant changes in the amount and activity of matrix metalloproteinase and vascular endothelial growth factor. Ligand binding to the active site of the protein was studied in computational causing conformational changes in the active site of the protein. Conclusion: Investigation revealed that the coumrins have inhibitory effects on the content and activity of MMP 2.9 and showed anti-angiogenetic effect. So, they can be potentially effective in the treatment of cancer. Interactive and competitive binding between MMP-9 and galbanic acid were studied with FT-IR, UV-Vis and fluorescence methods and MMP-9 structure was changed in these interactions.

  7. Eudesmane-Type Sesquiterpene Lactones Inhibit Nuclear Translocation of the Nuclear Factor κB Subunit RelB in Response to a Lymphotoxin β Stimulation.

    Science.gov (United States)

    Quach, Hue Tu; Kondo, Tetsuya; Watanabe, Megumi; Tamura, Ryuichi; Yajima, Yoshiki; Sayama, Shinsei; Ando, Masayoshi; Kataoka, Takao

    2017-01-01

    The transcription factor nuclear factor κB (NF-κB) regulates various biological processes, including inflammatory responses. We previously reported that eudesmane-type sesquiterpene lactones inhibited multiple steps in the canonical NF-κB signaling pathway induced by tumor necrosis factor-α and interleukin-1α. In contrast, the biological activities of eudesmane-type sesquiterpene lactones on the non-canonical NF-κB signaling pathway remain unclear. In the present study, we found that (11S)-2α-bromo-3-oxoeudesmano-12,6α-lactone, designated santonin-related compound 2 (SRC2), inhibited NF-κB luciferase reporter activity induced by lymphotoxin β (LTβ) in human lung carcinoma A549 cells. Although SRC2 did not prevent the processing of the NF-κB subunit p100 induced by LTβ, it inhibited the nuclear translocation of RelB and p52 in response to the LTβ stimulation. In contrast to (-)-dehydroxymethylepoxyquinomicin, SRC2 inhibited the LTβ-induced nuclear translocation of the RelB (C144S) mutant in a manner similar to wild-type RelB. While eudesmane derivatives possessing an α-bromoketone moiety or α,β-unsaturated carbonyl moieties inhibited LTβ-induced NF-κB luciferase reporter activity, eudesmane derivatives possessing an α-bromoketone moiety exhibited stronger inhibitory activity on the LTβ-induced nuclear translocation of RelB than those possessing a single α-methylene-γ-lactone moiety. The results of the present study revealed that SRC2 inhibits the nuclear translocation of RelB in the non-canonical NF-κB signaling pathway induced by LTβ.

  8. Pensamento, Teoria e Estudos Latino-americanos

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    Jacques de Novion

    2014-12-01

    Full Text Available RESUMO O presente trabalho configura como artigo inicial do Dossiê Especial – Pensamento, Teoria e Estudos Latino-americanos, organizado conjuntamente por nós, a pedido da Revista de Estudos e Pesquisas sobre as Américas (CEPPAC, do Instituto de Ciências Sociais (ICS, da Universidade de Brasília (UnB. De forma breve, este artigo apresenta a importância alcançada pelo Pensamento, pela Teoria, e, sobre tudo, pelos Estudos Latino-americanos nas últimas décadas. Em seguida, o artigo apresenta os quatorze trabalhos e uma resenha que compõem este Dossiê, resultado da contribuição de diferentes pesquisadores de distintas localidades da região, organizados em três blocos: Ciências Sociais Latino-americanas, Pensamento e Estudos. PALAVRAS CHAVE: Ciências Sociais Latino-americanas; Pensamento Latino-americanos; Teoria Latino-americana; Estudos Latino-americanos. --- RESUMEN El presente trabajo configura como articulo inicial del Dossier Especial – Pensamiento Teoría y Estudios Latinoamericanos, organizado conjuntamente por nosotros, a pedido de la Revista de Estudos e Pesquisas sobre as Américas (CEPPAC, del Instituto de Ciências Sociais (ICS, de la Universidade de Brasília (UnB. De forma breve, este articulo presenta la importancia alcanzada por el Pensamiento, por la Teoría, y, sobre todo, por los Estudios Latinoamericanos en las últimas décadas. En seguida, el articulo presenta los catorce trabajos e una reseña que componen este Dossier, resultado de la contribución de diferentes investigadores de distintas localidades de la región, organizados en tres bloques: Ciencias Sociales Latinoamericanas, Pensamiento y Estudios. PALABRAS CLAVE: Ciencias Sociales Latinoamericanas; Pensamiento Latinoamericano; Teoría Latinoamericana; Estudios Latinoamericanos. --- ABSTRACT This paper is set up as the initial article of this Special Dossier - Thought, Theory and Latin American Studies, which we organized collectively, at the request

  9. EMPREENDEDORISMO E VALORES HUMANOS: UM ESTUDO CONCEITUAL

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    Luciano Vignochi

    2013-09-01

    Full Text Available O objetivo principal deste artigo foi identificar conceitos de valores humanos em publicações científicas sobre empreendedorismo. Não há um único conceito de valores humanos, gerando diversas abordagens e aplicações do termo em estudos sobre empreendedorismo. Para identificar diferenças conceituais e aplicações práticas, optou-se por realizar uma análise de conteúdo em um portfólio de artigos selecionados em bases de dados. O principal critério de seleção das publicações foi aderência ao tema. Os resultados mostram que valores podem ser definidos como: critérios de julgamento da realidade; aspectos abstratos, cognitivos e afetivos ou características determinantes da personalidade e do comportamento de empreendedores. Valores influenciam a sobrevivência do indivíduo, o crescimento do empreendimento e podem nivelar a congruência do empreendedor e seu propósito de empreendimento com os critérios de convivência social. Ainda foram discriminados resultados de estudos aplicados com vistas a destacar evidências de contribuições práticas de pesquisas sobre valores humanos em empreendedorismo. Futuras investigações para aprofundar e replicar as análises realizadas neste estudo podem contribuir com a formação de empreendedores, a realização de diagnóstico empresarial e com avanços para a consolidação um modelo de desenvolvimento de empresas baseado em valores humanos.

  10. Novas tecnologias no estudo de ondas sonoras

    Directory of Open Access Journals (Sweden)

    Marisa Almeida Cavalcante

    2013-10-01

    Full Text Available http://dx.doi.org/10.5007/2175-7941.2013v30n3p579 O presente trabalho propõe a construção de um Tubo de Kundt adaptado às novas tecnologias disponíveis para o ensino de física. Tem como principal objetivo utilizar a placa Arduino em experimentos didáticos envolvendo o estudo de ondas sonoras. O Arduino é uma placa de controle I/O baseada no micro-controlador Atmega (Atmel e foi projetado inicialmente para fins didáticos. O fato da linguagem de programação utilizada e hardware serem do tipo open source (código aberto possibilitou sua ampla difusão em diversas áreas. Uma das intenções deste projeto é difundir o uso deste recurso para fins educacionais e particularmente no estudo de ondas sonoras estacionarias em tubos, contribuindo com a melhora na abordagem deste conteúdo no ensino e aprendizagem de Física.

  11. Determinantes da qualidade dos estudos de impacto ambiental

    OpenAIRE

    Almeida, Alexandre Nascimento de; Junior, Luiz Honorato da Silva; Nunes, Andre; Angelo, Humberto

    2015-01-01

    No Brasil, a realização de atividade que cause impacto ambiental significativo necessita licença ambiental. A obtenção dessa licença é vinculada a apresentação dos Estudos de Impacto Ambiental – EIA. O problema é que esses estudos tem apresentado uma baixa qualidade, contribuindo para tornar o processo de licenciamento menos efetivo. O objetivo do estudo é analisar os determinantes da qualidade dos Estudos de Impacto Ambiental. Para tanto, a partir das respostas de 74 questionários aplicados ...

  12. ESTUDO PROSPECTIVO SOBRE ENCAPSULAMENTO DE COMPOSTOS BIOATIVOS

    Directory of Open Access Journals (Sweden)

    Larissa Assunção

    2014-12-01

    Full Text Available Com o advento da nanotecnologia, houve o desenvolvimento de técnicas envolvendo processamento, fabricação e aplicação de estruturas, com forma e tamanho controlados, sendo que na indústria de alimentos, estas formulações, tanto na faixa micrométrica como nanométrica, vêm sendo utilizadas com o objetivo de favorecer o produto final. Tendo em vista a importância tecnológica dessa técnica e do grande interesse nacional e internacional, principalmente no ramo da indústria alimentícia, sobre os benefícios do encapsulamento, o presente estudo prospectivo teve como objetivo avaliar o panorama mundial relativo ao tema em questão, correlacionando-o com os documentos de patentes depositados. Para tanto, realizou-se uma pesquisa das patentes na base de dados europeia Espacenet®, utilizando os descritores do tema em estudo associados aos códigos de classificação. As informações encontradas foram compiladas em gráficos e discutidas. Pôde-se observar que o maior número de patentes referiu-se ao código A23L1/00 - alimentos ou produtos alimentícios: a sua preparação ou tratamento e houve um crescimento exponencial em relação ao depósito de patentes nos últimos 10 anos, sendo os Estados Unidos o país líder em número de patentes depositadas (60%. A Firmenich e CIE lidera o ranking com mais patentes depositadas. Em relação à área de atuação, predomina a indústria alimentícia (69%. No que diz respeito ao Brasil, o país aparece com apenas 1 patente detentora desta tecnologia, sendo um número bastante reduzido o que mostra a falta de incentivo neste setor. Diante dos resultados obtidos, concluiu-se que, apesar de ser uma tecnologia recente, observa-se uma tendência ao crescimento de depósito de patentes.

  13. Multi-analytical strategy for unassigned peaks using physical/mathematical separation, fragmental rules and retention index prediction: An example of sesquiterpene metabolites characterization in Cyperus rotundus.

    Science.gov (United States)

    He, Min; Yan, Pan; Yang, Zhiyu; Ye, Ying; Cao, Dongsheng; Hong, Liang; Yang, Tianbiao; Pei, Rui

    2018-05-30

    Comprehensive two-dimensional gas chromatography- mass spectrometry (GC × GC-qMS) can provide powerful physical separation, signal enhancement, and spectral identification for analytes in complex samples. Unassigned peaks are commonly presented in the untargeted profile after a single run with EI-MS spectral matching and retention index (RI) confirmation. The procedure proposed in this work can be applied as a general method for suggesting or narrowing down the candidates of unassigned GC × GC-qMS peaks. To begin, peak purity detection and chemometric resolution are employed to acquire pure mass spectra. In addition, the fragmental rules and in-silico spectra from structures are available for annotating certain unassigned peaks with reference spectra that are not observed in commercial databases. Furthermore, the procedure proposed in this work allows for in silico RI calculation by means of random forest (RF) analysis based on the retention data under the same chromatographic conditions. The calculated RIs can aid in analysis when the RI information of peaks of interest is not available in retention data libraries. Using the proposed strategy, certain unassigned peaks can be attributed to sesquiterpene metabolites in an in-house database for Cyperus rotundus. Copyright © 2018 Elsevier B.V. All rights reserved.

  14. Sesquiterpene lactone content and overall quality of fresh-cut witloof chicory (Cichorium intybus L. var. foliosum Hegi) as affected by different washing procedures.

    Science.gov (United States)

    Wulfkuehler, Sabine; Gras, Claudia; Carle, Reinhold

    2013-08-14

    Fresh-cut chicory was produced by four different processing lines testing cold and warm water (4/45 °C, 120 s) and cold water containing cysteine (0.5 mM) against an unwashed control. Levels of sesquiterpene lactones (SLs), sensory, and color attributes as well as O2 and CO2 levels in the modified atmosphere of the fresh-cut produce stored in consumer-sized bags (4 °C, 10 days) were monitored. All washing procedures applied significantly reduced the SL contents and bitterness in fresh-cut chicory. Warm water washing was most effective, reducing the total SL levels by 60.9-64.5%. Sensory and color attributes were also retained best by warm water washing, suggesting such treatments to be suitable measures to improve the quality and reduce the bitterness of fresh-cut chicory. SL profiles changed substantially in all samples during storage, revealing lactucopicrin to be the major SL directly after processing, whereas 11(S),13-dihydrolactuin prevailed at the end of storage.

  15. Differential effects of Helenalin, an anti-inflammatory sesquiterpene lactone, on the proteome, metabolome and the oxidative stress response in several immune cell types.

    Science.gov (United States)

    Zwicker, Paula; Schultze, Nadin; Niehs, Sarah; Albrecht, Dirk; Methling, Karen; Wurster, Martina; Wachlin, Gerhild; Lalk, Michael; Lindequist, Ulrike; Haertel, Beate

    2017-04-01

    Extracts of Arnica spp. are traditionally used due to their anti-inflammatory effects for the topical treatment of e.g. haematoma or muscle distortions. One of the main active compounds is Helenalin, a sesquiterpene lactone that can be found in various Asteraceae. However, immunotoxic effects of the compound are only poorly analysed. In this study, a 2D gel electrophoresis based proteomic approach together with a membrane based proteomic assay, metabolomics and the detection of intracellular reactive oxygen species (iROS) were used to investigate potential immunotoxic properties of Helenalin on the human immune cell lines Jurkat and THP-1 and on human peripheral blood mononuclear cells (PBMC). The study revealed a dose-dependent cytotoxicity towards both tested cell lines and the PBMC. However, the cell lines were less sensitive to the Helenalin treatment than the PBMC. The proteomic assays showed strong effects on the carbohydrate metabolism and the protein folding in THP-1 cells but only weak impact on Jurkat cells. Metabolomic studies as well as iROS detection in THP-1 cells verified the results of the proteomic analysis. In summary, the approaches used in this study were able to identify target pathways of Helenalin especially in THP-1 monocytes and thus enable a risk assessment of the substance. Copyright © 2016. Published by Elsevier Ltd.

  16. A dimeric urea of the bisabolene sesquiterpene from the Okinawan marine sponge Axinyssa sp. inhibits protein tyrosine phosphatase 1B activity in Huh-7 human hepatoma cells.

    Science.gov (United States)

    Abdjul, Delfly B; Kanno, Syu-Ichi; Yamazaki, Hiroyuki; Ukai, Kazuyo; Namikoshi, Michio

    2016-01-15

    Protein tyrosine phosphatase 1B (PTP1B) plays an important role as a negative regulator of the insulin and leptin signaling pathways. Therefore, this enzyme is regarded as an attractive therapeutic target for the treatment of type 2 diabetes and obesity. Our screening program for PTP1B inhibitors led to the isolation of four sesquiterpenes and sterol: N,N'-bis[(6R,7S)-7-amino-7,8-dihydro-α-bisabolen-7-yl]urea (1), (6R,7S)-7-amino-7,8-dihydro-α-bisabolene (2), (1R,6S,7S,10S)-10-isothiocyanato-4-amorphene (3), axinisothiocyanate J (4), and axinysterol (5) from the marine sponge Axinyssa sp. collected at Iriomote Island. Of these, compound 1 was the most potent inhibitor of PTP1B activity (IC50=1.9μM) without cytotoxicity at 50μM in two human cancer cell lines, hepatoma Huh-7 and bladder carcinoma EJ-1 cells. Compound 1 also moderately enhanced the insulin-stimulated phosphorylation levels of Akt in Huh-7 cells. Therefore, compound 1 has potential as a new type of anti-diabetic drug candidate possessing PTP1B inhibitory activity. Copyright © 2015 Elsevier Ltd. All rights reserved.

  17. Extracts of three Laserpitium L. species and their principal components laserpitine and sesquiterpene lactones inhibit microbial growth and biofilm formation by oral Candida isolates.

    Science.gov (United States)

    Popović, Višnja; Stojković, Dejan; Nikolić, Miloš; Heyerick, Arne; Petrović, Silvana; Soković, Marina; Niketić, Marjan

    2015-04-01

    Antimicrobial properties of extracts of underground parts of three Laserpitium L. (Apiaceae) species, namely Laserpitium latifolium L., Laserpitium zernyi Hayek and Laserpitium ochridanum Micevski, were investigated. The investigated species are widely used as functional foods, as spices and for preparations in traditional medicine for treating complaints connected with infection and inflammation. Furthermore, antimicrobial and antibiofilm effects of laserpitine, the most abundant compound in the chloroform extract of L. latifolium, and guaianolide sesquiterpene lactones, such as, isomontanolide, montanolide and tarolide, principal components of the extracts of L. zernyi and L. ochridanum were assessed. The antimicrobial activity was tested using the microdilution method against five pathogenic bacteria and five fungi, as well as in the microplate biofilm assay on two Candida clinical isolates (C. albicans and C. krusei). Among the extracts, L. latifolium showed the most prominent activity. Isolated metabolites exerted higher effects against fungal than against bacterial strains, isomontanolide being the most active. Interestingly, all constituents showed higher potential on inhibition of biofilm formation than fluconazole, a reference compound. Tested metabolites may be good novel agents with high antifungal and antibacterial potential that might find practical applications in food industry as food preservatives in order to retard the growth of food spoiling microbes, but only after detailed safety assessments.

  18. Induction of G2/M arrest and apoptosis through mitochondria pathway by a dimer sesquiterpene lactone from Smallanthus sonchifolius in HeLa cells.

    Science.gov (United States)

    Kitai, Yurika; Zhang, Xia; Hayashida, Yushi; Kakehi, Yoshiyuki; Tamura, Hirotoshi

    2017-07-01

    Dimer sesquiterpene lactones (SLs), uvedafolin and enhydrofolin, against four monomer SLs isolated from yacon, Smallanthus sonchifolius, leaf were the most cytotoxic substances on HeLa cells (IC 50 values 2.96-3.17 μM at 24 hours). However, the cytotoxic mechanism of dimer SL has not been elucidated yet. Therefore, in this study, we clarified the in vitro cytotoxic mechanism of uvedafolin on the HeLa cells, and evaluated the cytotoxicity against NIH/3T3 cells which were used as normal cells. In consequence, the dimer SLs had low toxicity for the NIH/3T3 cells (IC 50 4.81-4.98 μM at 24 hours) and then the uvedafolin mediated cell cycle arrest at the G 2 /M phase and induced apoptosis on the HeLa cells evidenced by appearance of a subG1 peak. Uvedafolin induced apoptosis was attributed to caspase-9 and caspase-3/7 activities. An effectively induced apoptosis pathway was demonstrated from mitochondria membrane potential change and cytochrome c release to cytosol. These results reveal that uvedafolin induced apoptosis via the mitochondria pathway. The present results indicate the potential of uvedafolin as a leading compound of new anticancer agents. Copyright © 2016. Published by Elsevier B.V.

  19. Induction of G2/M arrest and apoptosis through mitochondria pathway by a dimer sesquiterpene lactone from Smallanthus sonchifolius in HeLa cells

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    Yurika Kitai

    2017-07-01

    Full Text Available Dimer sesquiterpene lactones (SLs, uvedafolin and enhydrofolin, against four monomer SLs isolated from yacon, Smallanthus sonchifolius, leaf were the most cytotoxic substances on HeLa cells (IC50 values 2.96–3.17 μM at 24 hours. However, the cytotoxic mechanism of dimer SL has not been elucidated yet. Therefore, in this study, we clarified the in vitro cytotoxic mechanism of uvedafolin on the HeLa cells, and evaluated the cytotoxicity against NIH/3T3 cells which were used as normal cells. In consequence, the dimer SLs had low toxicity for the NIH/3T3 cells (IC50 4.81–4.98 μM at 24 hours and then the uvedafolin mediated cell cycle arrest at the G2/M phase and induced apoptosis on the HeLa cells evidenced by appearance of a subG1 peak. Uvedafolin induced apoptosis was attributed to caspase-9 and caspase-3/7 activities. An effectively induced apoptosis pathway was demonstrated from mitochondria membrane potential change and cytochrome c release to cytosol. These results reveal that uvedafolin induced apoptosis via the mitochondria pathway. The present results indicate the potential of uvedafolin as a leading compound of new anticancer agents.

  20. Paralisia periodica familiar: estudo de oito casos

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    José Lamartine De Assis

    1980-12-01

    Full Text Available Foram estudados oito pacientes com Paralisia Periódica Familiar sob os aspectos clínico, hidro-eletrolítico, eletrofisiológico, histológico e terapêutico. Houve predomínio significante em pacientes do sexo masculino. A idade média do início da moléstia foi de 15 anos. As manifestações clínicas foram concor-dantes com as referidas na literatura. Todos os pacientes tinham a forma hipocalêmica exceto um que evoluiu com hipercalemia. Alguns pacientes (21,4% tinham natremias no limite superior da normalidade enquanto em um número igual de pacientes as natremias estavam elevadas, o que sugeria liberação de aldosterona. Os achados eletrofisiológicos foram concordantes com os da literatura. O estudo histológico de músculos realizado em cinco pacientes foi normal em 30% deles e, naqueles com crises repetidas e freqüentes, foram encontradas atrofias de fibras musculares. Foram feitos tratamentos preventivos, e nos pacientes com hipocalemia foram administradas doses suplementares de potássio em solução aquosa de 10-25% diariamente.

  1. Esclerose tuberosa: estudo multidisciplinar de 15 casos

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    E. M. T. Yacubian

    1983-06-01

    Full Text Available São apresentados os resultados de estudo multidisciplinar de 15 pacientes com esclerose tuberosa, 8 do sexo masculino e 7 do sexo feminino, com idades entre 19 meses e 23 anos; 11 foram considerados como casos esporádicos e, em 4, não foi possível precisar os antecedentes familiares. Foram observadas as seguintes manifestações cutâneas: adenoma de Pringle em 9 casos; manchas acrômicas em 9 casos; fibroma periungueal em 3 casos. Dos 15 casos 13 apresentaram D.M., deficiência mental, enquanto os outros 2 tinham inteligência limítrofe. Com exceção de 2 pacientes, todos tinham crises epilépticas e os tipos de crises mais frequentemente observados foram as ausências atípicas e as crises tônicas. Espasmos infantis estavam presentes em 3 casos e em apenas um caso o eletrencefalograma apresentou aspecto hipsarrítmico. A tomografia axial computadorizada, realizada em 7 casos, revelou calcificações periventriculares em todos, mesmo naqueles que apresentaram RX simples de crânio normal (4 casos.

  2. Bibliotecas domiciliares: um estudo de caso

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    Adalberto Rego Maciel Filho

    Full Text Available Pesquisa empírica sobre o papel da biblioteca domiciliar e do hábito de leitura dos pais dos professores da Universidade de Pernambuco, na escolaridade dos educadores. 107 professores foram entrevistados (24 especialistas, 60 mestres, 25 doutores. Constatou-se que a residência na infância dos professores da UPE, na média, teria uma biblioteca domiciliar de 119,7 volumes (no Fundamental I; e seus pais, na maioria, liam e tinham quase 11,1 anos de estudo. Simulações também foram realizadas, analisando as relações entre o tamanho das bibliotecas domiciliares e desempenho escolar com dados do PISA, referentes a 27 países e, no caso do Brasil, com 2000 alunos. Os resultados acompanham as conclusões apresentadas na revisão da literatura, que apontam uma correlação positiva entre as duas variáveis. São sugeridas políticas que poderiam influenciar programas já existentes e novos projetos, ou, ainda, incentivar novas pesquisas na área de bibliotecas domiciliares e públicas.

  3. Legendagem comercial e lengendagem pirata: um estudo comparado

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    Marcos Pereira Feitosa

    2009-01-01

    Este trabalho situa-se no campo disciplinar dos Estudos da Tradução, mais especificamente, dos Estudos da Tradução Audiovisual, com um corpus composto de fragmentos iniciais de dez filmes de terror/suspense produzidos em língua inglesa na década atual com suas respectivas legendagens a) comerciais e b) piratas (fansubs, fantitles ou "legendas de fãs para fãs") em português. Foram utilizadas categorias i) dos estudos de aspectos descritivos de modalidades da Tradução Audiovisual (DÍAZ-CINTAS, ...

  4. Marketing do mercado de luxo: Estudo de caso Victoria's Secret

    OpenAIRE

    Ana Francisca Giuliani

    2013-01-01

    Este artigo teve por objetivo identificar quais são as estratégias de marketing utilizadas pela empresa varejista Victoria’s Secret para a fidelização do consumidor de luxo em nível global. O estudo caracterizou-se como uma pesquisa qualitativa, utilizando-se como estratégia de campo o estudo de caso de caráter ilustrativo. Para o alcance do objetivo proposto, dividiu-se o estudo em duas fases. Na primeira, denominada exploratória, buscou-se em dados secundários o embasamento para a construçã...

  5. Reticulossarcomas epidurais intraespinais: estudo de 34 casos

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    Lígia M. B. Coutinho

    1974-12-01

    Full Text Available Foi realizado um estudo de 34 casos de reticulossarcomas localizados no tecido epidural intraespinal. Assinala-se sua maior freqüência entre homens com mais de 50 anos, embora pacientes mais jovens possam ser acometidos, principalmente entre 15 e 29 anos. A distribuição segmentar dos tumores permite um diagnóstico diferencial, sendo a região torácica a sede mais freqüentes de sarcomas. O principal sintoma encontrado nos pacientes com reticulossarcoma foi a dor, do tipo radicular, que serve para diferenciar os tumores intra e extra-medulares, tendo sido de aparecimento precoce em 15 dos pacientes estudados, precedendo em muitos meses e até anos os demais sintomas. Classificou-se os tumores reticulohistiocitários epidurais do canal vertebral em três grupos, de acordo com seu aspecto tecidual e celular e, principalmente, com a presença e distribuição da reticulina. Relacionou-se o tempo de evolução desses tumores com seu aspecto histopatológico, chegando-se à conclusão que os tumores classificados no grupo III apresentam um período evolutivo maior que os demais, o que nos levou a pensar que crescem mais lentamente, apresentando, portanto, melhor prognóstico que os demais tipos, muito embora todos os tipos de reticulossarcomas possam apresentar um prognóstico mais favorável quando precocemente diagnosticados e tratados adequadamente.

  6. Histerectomias: estudo retrospectivo de 554 casos

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    Eddie Fernando Cândido Murta

    Full Text Available OBJETIVO: A histerectomia é uma operação muito realizada, entretanto há poucos trabalhos na literatura nacional sobre suas indicações, técnica e complicações. O objetivo deste trabalho é avaliar estes procedimentos realizados na Disciplina de Ginecologia e Obstetrícia da Faculdade de Medicina do Triângulo Mineiro. MÉTODO: Estudo retrospectivo de 470 histerectomias abdominais e 84 vaginais foi conduzido analisando as indicações, tempo de cirurgia e internação, tipo de incisão e morbidez. RESULTADOS: As principais indicações foram o mioma uterino e o prolapso uterino para as histerectomias abdominais e vaginais, respectivamente. As complicações intra-operatórias aconteceram em 3,4% e as pós-operatórias em 2,4% do total de casos. Nenhuma diferença estatística foi encontrada no número de complicações em relação ao tipo de incisão (vertical ou transversal. O tempo de cirurgia e o de hospitalização foram estatisticamente maiores nas incisões verticais. A hemorragia foi a mais freqüente complicação intra-operatória e a infecção da incisão operatória foi a mais freqüente no pós-operatório. CONCLUSÕES: A histerectomia é um procedimento de baixo risco, no entanto, a realização de revisões sobre indicações e complicações, e a pesquisa de melhores técnicas cirúrgicas são necessárias para torná-la cada vez mais segura.

  7. Estudos sobre a Esquistosomose em Pernambuco, Brasil

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    Aggeu Magalhães

    1940-01-01

    Full Text Available Os autores referem os resultados dos estudos sobre esquistosomose realisados, em Pernambuco, de Novembro de 1938 a Dezembro de 1939. Não se extendem em discussão, nem comentarios, tampouco, relacionam suas verificações com o que já foi relatado sobre o assunto em trabalhos nacionais e estrangeiros, pelo fato de que, não se trata de um resultado definitivo e sim de atividades que irão proseguir. Os estudos sistematicos em torno da endemia esquistosomica foram executados em duas localidades diversas pela população e pela situação geografica e ecologica. Pontezinha é uma povoação de 1200 habitantes localizada proximo a um conjunto de charcos e lagõas de agua dôce onde pululam caramujos do tipo olivaceus de Spix, e apresenta uma taxa de infestação de 21,4% para individuos do sexo masculino, e de 14,7% para os do sexo feminino. A distribuição por grupos de idade mostra que a incidencia cresce até o grupo de 16 a 20 anos de idade, quando atinge a percentagem de 37,8%, para decrescer em seguida. Em Vitoria a incidencia foi maior nos indivíduos do sexo feminino, atingindo a taxa de 41,4%, dando o sexo masculino a taxa de 36,9%. Por idade a percentagem maxima é atingida pelo grupo de 11 a 15 anos. Vitoria é uma cidade de 1500 habitantes, construida á margem do rio Tapacurá, onde se encontram numerosos caramujos do tipo centimetralis Lutz. A divergencia de incidencia corre por conta dos habitos da população e pela utilização que ela faz do rio, sendo maior em Vitoria porque a proximidade do rio facilita o uso de suas aguas para banho e serviços domesticos, condicionando este ultimo fato, a maior infestação das mulheres. O estudo da frequencia da infestação dos caramujos por cercarias de diversos trematodios, permite suspeitar uma relação inversa entre a taxa de infestação e o diametro maximo atingido pelos caramujos, parecendo este fato confirmar os trabalhos de Vianna Martins sobre a identidade dos hospedeiros

  8. Gerencia de Informacao em Estudos Multicentricos: o Estudo Longitudinal de Saude do Adulto

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    Bruce Bartholow Duncan

    2013-06-01

    Full Text Available A gerência da informação em estudos multicêntricos de grande porte requer uma abordagem especializada. O Estudo Longitudinal da Saúde do Adulto (ELSA-Brasil criou um Centro de Dados para delinear e gerenciar seu sistema de dados. O objetivo do artigo foi descrever os passos envolvidos, incluindo os métodos de entrada, transmissão e gerência de informações. Foi desenvolvido um sistema web que permitiu, de forma segura e confidencial, a entrada online, verificação e edição, bem como incorporação de dados coletados em papel. Além disso, foi implantado e personalizado um sistema de armazenamento e comunicação de imagens (Picture Arquiving and Communication System para ecocardiografia e retinografia que armazena as imagens recebidas dos Centros de Investigação e as torna acessíveis nos Centros de Leitura. Finalmente, foram desenvolvidos processos de extração e limpeza de dados para criação de bases de dados em formatos que permitam análises em múltiplos pacotes estatísticos.

  9. Two sesquiterpene aminoquinones protect against oxidative injury in HaCaT keratinocytes via activation of AMPKα/ERK-Nrf2/ARE/HO-1 signaling.

    Science.gov (United States)

    Liu, Li; Wu, Wei; Li, Jing; Jiao, Wei-Hua; Liu, Li-Yun; Tang, Jie; Liu, Lei; Sun, Fan; Han, Bing-Nan; Lin, Hou-Wen

    2018-04-01

    To investigate the cytoprotective effects of two sesquiterpene aminoquinones isolated from the marine sponge Dysidea fragilis, Dysidaminone H (DA8) and 3'-methylamino-avarone (DA14), we examined their effects against hydrogen peroxide (H 2 O 2 )-induced oxidative injury in human keratinocyte cell line and elucidated the underlying mechanisms. Cell viability was detected using a CCK-8 assay kit. Intracellular reactive oxygen species (ROS) production was measured by fluorescence of 2, 7-Dichlorodi-hydrofluorescein diacetate (DCFH-DA). Messenger RNA and protein expression were measured by real-time quantitative PCR and western blotting analysis. Immunocytochemistry was performed to determine the intracellular location of nuclear factorerythroid 2 p45 related factor 2 (Nrf2). The antioxidant response element (ARE)-luciferase reporter gene assay and RNA interference were used to establish the role of ARE and Nrf2. DA8 and DA14 (DAs) resisted H 2 O 2 induced decline of cell viability by inhibiting the accumulation of ROS. Meanwhile, DAs increased HO-1 expression and ARE activity and induced Nrf2 expression, as well as the accumulation of Nrf2 in the cell nucleus. However, silencing of Nrf2 abolished DAs-induced HO-1 expression and ARE luciferase activation. In addition, DAs induced the phosphorylation of both cyclic AMP-activated protein kinase-α (AMPKα) and extracellular signal-regulated kinase (ERK), while specific inhibitors of AMPKα and ERK abrogated HO1 upregulation and Nrf2 activation. DAs provided cytoprotective effects against H 2 O 2 -induced cytotoxicity by activation of the Nrf2/ARE/HO-1 pathway via phosphorylation of AMPKα and ERK. The findings suggested that DA8 and DA14 might be the candidate therapeutic agents for skin diseases caused by oxidative injury. Copyright © 2018 Elsevier Masson SAS. All rights reserved.

  10. Anti-ulcerogenic mechanisms of the sesquiterpene lactone onopordopicrin-enriched fraction from Arctium lappa L. (Asteraceae): role of somatostatin, gastrin, and endogenous sulfhydryls and nitric oxide.

    Science.gov (United States)

    de Almeida, Ana Beatriz Albino; Luiz-Ferreira, Anderson; Cola, Maíra; Di Pietro Magri, Luciana; Batista, Leonia Maria; de Paiva, Joseilson Alves; Trigo, José Roberto; Souza-Brito, Alba R M

    2012-04-01

    Arctium lappa L. has been used in folk medicine as a diuretic, depurative, and digestive stimulant and in dermatological conditions. The mechanisms involved in the anti-ulcerogenic activity of the sesquiterpene onopordopicrin (ONP)-enriched fraction (termed the ONP fraction), obtained from A. lappa leaves, were studied. The gastroprotective mechanism of the ONP fraction was evaluated in experimental in vivo models in rodents, mimicking this disease in humans. ONP fraction (50 mg/kg, p.o.) significantly inhibited the mucosal injury induced by ethanol/HCl solution (75%), indomethacin/bethanecol (68.9%), and stress (58.3%). When the ONP fraction was investigated in pylorus ligature, it did not induce alteration in the gastric volume but did modify the pH and total acid concentration of gastric juice. ONP fraction significantly increased serum somatostatin levels (82.1±4.1 vs. control group 12.7±4 pmol/L) and decreased serum gastrin levels (62.6±6.04 vs. control group 361.5±8.2 μU/mL). Mucus production was not significantly altered by the ONP fraction. Gastroprotection by the ONP fraction was completely inhibited by N-ethylmaleimide treatment and did not modify the effect in the animals pretreated with l-N(G)-nitroarginine methyl ester. These results suggest an antisecretory mechanism involved with the antiulcerogenic effect of the ONP fraction. However, only endogenous sulfhydryls play an important role in gastroprotection of the ONP fraction.

  11. Effects of some sesquiterpenes on the stored-product insect Tenebrio molitor (Coleoptera: Tenebrionidae Efectos de algunos sesquiterpenos sobre el insecto de productos almacenados, Tenebrio molitor (Coleoptera: Tenebrionidae

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    Matías García

    2003-12-01

    Full Text Available In order to evaluate the allelochemical activity of some sesquiterpenes isolated from the native plant Tessaria absinthioides (Hook. et Arn. DC, and some semi synthetic derivatives against Tenebrio molitor L. larvae, we have developed bioassays directed to quantify repellency, larval mortality, and its effects on the development. Although costic aldehyde caused the maximum repellent effect, all the compounds showed a significant effect at some dose or time, indicating behavioral avoidance. The topical application of costic aldehyde produced the largest increase on the duration of the pupal stage. Tessaric acid exhibited the highest toxicity by topical application at the experiment closure. Both eremophilane-1(10,2,11(13-triene-12-oic, and -costic acids induced some morphological deformities.Con el objeto de evaluar sesquiterpenos aislados de la planta nativa Tessaria absinthioides (Hook et Arn y algunos derivados semisintéticos frente a larvas de Tenebrio molitor L., se desarrollaron bioensayos orientados a la cuantificación de la repelencia, mortalidad de larvas y efectos sobre el desarrollo. Aldehído cóstico produjo el mayor incremento en la duración del estado pupal por aplicación tópica. Acido tessárico exhibió el más alto porcentaje de mortalidad al finalizar el período de experimentación. Los productos eremophilan-1(10,2, 11(13-trien-12-oico y ácido -cóstico dieron lugar al mayor número de malformaciones. Si bien aldehído cóstico mostró la máxima actividad de repelencia, todos los compuestos evaluados produjeron efectos significativos en el ensayo de elección.

  12. Foliar mono- and sesquiterpene contents in relation to leaf economic spectrum in native and alien species in Oahu (Hawai'i).

    Science.gov (United States)

    Sardans, Jordi; Llusià, Joan; Niinemets, Ulo; Owen, Sue; Peñuelas, Josep

    2010-02-01

    Capacity for terpene production may confer advantage in protection against abiotic stresses such as heat and drought, and also against herbivore and pathogen attack. Plant invasive success has been intense in the Hawaiian islands, but little is known about terpene content in native and alien plant species on these islands. We conducted a screening of leaf terpene concentrations in 35 native and 38 alien dominant plant species on Oahu island. Ten (29%) of the 35 native species and 15 (39%) of the 38 alien species contained terpenes in the leaves. This is the first report of terpene content for the ten native species, and for 10 of the 15 alien species. A total of 156 different terpenes (54 monoterpenes and 102 sesquiterpenes) were detected. Terpene content had no phylogenetic significance among the studied species. Alien species contained significantly more terpenes in leaves (average+/-SE=1965+/-367 microg g(-1)) than native species (830+/-227 microg g(-1)). Alien species showed significantly higher photosynthetic capacity, N content, and lower Leaf Mass Area (LMA) than native species, and showed higher total terpene leaf content per N and P leaf content. Alien species, thus, did not follow the expected pattern of "excess carbon" in comparison with native species. Instead, patterns were consistent with the "nutrient driven synthesis" hypothesis. Comparing alien and native species, the results also support the modified Evolution of Increased Competitive Ability (EICA) hypothesis that suggests that alien success may be favored by a defense system based on an increase in concentrations of less costly defenses (terpenes) against generalist herbivores.

  13. The sesquiterpene (-)-α-bisabolol is active against the causative agents of Old World cutaneous leishmaniasis through the induction of mitochondrial-dependent apoptosis.

    Science.gov (United States)

    Corpas-López, V; Merino-Espinosa, G; Díaz-Sáez, V; Morillas-Márquez, F; Navarro-Moll, M C; Martín-Sánchez, J

    2016-10-01

    Cutaneous leishmaniasis treatment remains challenging due to the absence of a satisfactory treatment. The screening of natural compounds is a valuable strategy in the search of new drugs against leishmaniasis. The sesquiterpene (-)-α-bisabolol is effective in vivo against visceral leishmaniasis due to Leishmania infantum, but its mechanism of action remains elusive. The aim of this study is to validate this promising compound against the causative species of Old World cutaneous leishmaniasis and to get an insight into its antileishmanial mode of action. The compound was evaluated on L. tropica promastigotes and intracellular amastigotes using bone marrow-derived macrophages and its cytotoxicity was evaluated on L929 fibroblasts. The reactive oxygen species generation was evaluated using a sensitive probe. Mitochondrial depolarization was assessed evaluating the fluorescence due to rhodamine 123 in a flow cytometer. Apoptosis was investigated by measuring the fluorescence due to annexin V and propidium iodide in a flow cytometer. The ultrastructure of treated promastigotes and intracellular amastigotes was analysed through transmission electron microscopy. (-)-α-Bisabolol was active against L. tropica intracellular amastigotes displaying an inhibitory concentration 50 % of 25.2 µM and showing low cytotoxicity. This compound induced time and dose-dependent oxidative stress, mitochondrial depolarization and phosphatidilserine externalization (a marker of apoptosis). These effects were noticed at a low concentration and short exposure time. In the ultrastructural analyses, the treated parasites showed mitochondrial disruption, presence of electron-dense structures and chromatin condensation. These results suggest that this natural compound induces oxidative stress and mitochondrial-dependent apoptosis on Leishmania without disturbing the plasma membrane.

  14. Cytochrome P450s from Cynara cardunculus L. CYP71AV9 and CYP71BL5, catalyze distinct hydroxylations in the sesquiterpene lactone biosynthetic pathway.

    Science.gov (United States)

    Eljounaidi, Kaouthar; Cankar, Katarina; Comino, Cinzia; Moglia, Andrea; Hehn, Alain; Bourgaud, Frédéric; Bouwmeester, Harro; Menin, Barbara; Lanteri, Sergio; Beekwilder, Jules

    2014-06-01

    Cynara cardunculus (Asteraceae) is a cross pollinated perennial crop which includes the two cultivated taxa globe artichoke and cultivated cardoon. The leaves of these plants contain high concentrations of sesquiterpene lactones (STLs) among which cynaropicrin is the most represented, and has recently attracted attention because of its therapeutic potential as anti-tumor and anti-photoaging agent. Costunolide is considered the common precursor of the STLs and three enzymes are involved in its biosynthetic pathway: i.e. the germacrene A synthase (GAS), the germacrene A oxidase (GAO) and the costunolide synthase (COS). Here we report on the isolation of two P450 genes, (i.e. CYP71AV9 and CYP71BL5), in a set of ∼19,000 C. cardunculus unigenes, and their functional characterization in yeast and in planta. The metabolite analyses revealed that the co-expression of CYP71AV9 together with GAS resulted in the biosynthesis of germacra-1(10),4,11(13)-trien-12-oic acid in yeast. The co-expression of CYP71BL5 and CYP71AV9 with GAS led to biosynthesis of the free costunolide in yeast and costunolide conjugates in Nicotiana benthamiana, demonstrating their involvement in STL biosynthesis as GAO and COS enzymes. The substrate specificity of CYP71AV9 was investigated by testing its ability to convert amorpha-4,11-diene, (+)-germacrene D and cascarilladiene to their oxidized products when co-expressed in yeast with the corresponding terpene synthases. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  15. ESTUDOS NUTRICIONAIS COM ARROZ (Oryza sativa, L.

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    Alfredo LAM-SÁNCHEZ

    2009-07-01

    Full Text Available

    RESUMO: Estudos com arroz (Oryza sativa, L. foram realizados a fim de determinar a variabilidade do teor de proteína em coleções de cultivares e a possibilidade de utilizar as camadas periféricas do grão na alimentação humana. Foram analisadas 626 linhagens e cultivares de arroz que mostraram uma variação quanto ao teor de proteína de 4.00 a 14.31 g/100 g de amostra. Para se extrair as camadas periféricas do grão, foi desenvolvida uma máquina experimental, que removia as camadas superficiais dos grãos de arroz integral (inteiros somente com a casca removida. Verificou-se que a quantidade de proteína do grão diminui de fora para dentro. O resíduo, que era constituído de grande parte de restos de endosperma com um teor protéico menor mas incluía o embrião, que é a primeira estrutura a se deslocar de grão no beneficiamento e contém proteínas, vitaminas, sais minerais e lípides, tinha um bom valor nutritivo. Desenvolveu-se também uma fórmula para alimentação infantil a partir da mistura de leite de vaca com arroz, com composição semelhante à do leite materno, a ser utilizada sob a forma de bebida ou mingau. Formulações com 40%-20% arroz + 60%-80% leite de vaca proporcionaram valores de Coeficiente de Eficiência Protéica (2,39 e 2,38, respectivamente que não diferiam estatisticamente do valor de 2,43 obtido na fórmula com 100% de leite de vaca. Seus preços foram 59% e 65% do obtido para o leite de vaca integral. PALAVRAS-CHAVE: Arroz; qualidade do grão; proteína; camadas periféricas; misturas arroz-leite; nutrição humana.

  16. Estudos organizacionais, (descolonialidade e estudos da dependência: as contribuições da Cepal

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    Sergio Wanderley

    Full Text Available O objetivo deste artigo é (revisitar o conceito de dependência como categoria de investigação da (e a partir da América Latina, por meio de uma perspectiva histórica crítica descolonial, a fim de ampliar o espaço de debates em estudos organizacionais e promover alternativas à ordem neoliberal. Este ensaio terá como foco os estudos da dependência realizados pela Comissão Econômica para a América Latina e o Caribe (Cepal durante a década de 1950 e o início dos anos 1960. A perspectiva descolonial é um constructo teórico de autores latino-americanos que se consideram herdeiros da longa tradição do pensamento social crítico da região, na qual a teoria da dependência está inserida. As propostas dos autores da dependência confrontaram as principais teorias ortodoxas do Norte produzidas à época. A partir do conceito de centro-periferia, da denúncia de assimetrias nas relações entre essas regiões, do reconhecimento da interdependência entre desenvolvimento e subdesenvolvimento, o conceito de dependência foi sendo (reelaborado nas décadas de 1950 e 1960 por diversos autores latino-americanos como uma categoria de investigação da realidade da (e a partir da região. Essas investigações tinham por objetivo não somente elaborar constructos teóricos, mas, também, transformar a realidade por meio da criação de diversas organizações e instituições que deveriam servir ao propósito de superar o subdesenvolvimento. A denúncia da historicidade da situação de subdesenvolvimento desnudou o caráter de neutralidade do economicismo das teorias produzidas no Norte e promoveu um encontro teórico entre economia e política que muito tem a contribuir com a área dos estudos organizacionais.

  17. Sesquiterpene dimmer (DSF-27) inhibits the release of neuroinflammatory mediators from microglia by targeting spleen tyrosine kinase (Syk) and Janus kinase 2 (Jak2): Two major non-receptor tyrosine signaling proteins involved in inflammatory events

    Energy Technology Data Exchange (ETDEWEB)

    Zeng, Ke-Wu [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191 (China); Wang, Shu [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191 (China); Department of Medicinal Chemistry and Pharmaceutical Analysis, Logistics College of Chinese People' s Armed Police Forces, Tianjin 300162 (China); Dong, Xin; Jiang, Yong; Jin, Hong-Wei [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191 (China); Tu, Peng-Fei, E-mail: pengfeitu@vip.163.com [State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191 (China)

    2014-03-15

    Non-receptor protein tyrosine kinases (NRPTKs)-dependent inflammatory signal transduction cascades play key roles in immunoregulation. However, drug intervention through NRPTKs-involved immunoregulation mechanism in microglia (the major immune cells of the central nervous system) has not been widely investigated. A main aim of the present study is to elucidate the contribution of two major NRPTKs (Syk and Jak2) in neuroinflammation suppression by a bioactive sesquiterpene dimmer (DSF-27). We found that LPS-stimulated BV-2 cells activated Syk and further initiated Akt/NF-κB inflammatory pathway. This Syk-dependent Akt/NF-κB inflammatory pathway can be effectively ameliorated by DSF-27. Moreover, Jak2 was activated by LPS, which was followed by transcriptional factor Stat3 activation. The Jak2/Stat3 signal was suppressed by DSF-27 through inhibition of Jak2 and Stat3 phosphorylation, promotion of Jak/Stat3 inhibitory factors PIAS3 expression, and down-regulation of ERK and p38 MAPK phosphorylation. Furthermore, DSF-27 protected cortical and mesencephalic dopaminergic neurons against neuroinflammatory injury. Taken together, our findings indicate NRPTK signaling pathways including Syk/NF-κB and Jak2/Stat3 cascades are potential anti-neuroinflammatory targets in microglia, and may also set the basis for the use of sesquiterpene dimmer as a therapeutic approach for neuroinflammation via interruption of these pathways. - Highlights: • Sesquiterpene dimmer DSF-27 inhibits inflammatory mediators' production in microglia. • Syk-dependent Akt/NF-κB pathway is important for DSF-27's anti-inflammation activity. • Jak2/Stat3 pathway is important for DSF-27's anti-inflammation activity. • Jak2/Stat3 signaling pathway is partly regulated by ERK and p38 MAPKs and PIAS3. • DSF-27 protects neurons against microglia-mediated neuroinflammatory injury.

  18. Sesquiterpene dimmer (DSF-27) inhibits the release of neuroinflammatory mediators from microglia by targeting spleen tyrosine kinase (Syk) and Janus kinase 2 (Jak2): Two major non-receptor tyrosine signaling proteins involved in inflammatory events

    International Nuclear Information System (INIS)

    Zeng, Ke-Wu; Wang, Shu; Dong, Xin; Jiang, Yong; Jin, Hong-Wei; Tu, Peng-Fei

    2014-01-01

    Non-receptor protein tyrosine kinases (NRPTKs)-dependent inflammatory signal transduction cascades play key roles in immunoregulation. However, drug intervention through NRPTKs-involved immunoregulation mechanism in microglia (the major immune cells of the central nervous system) has not been widely investigated. A main aim of the present study is to elucidate the contribution of two major NRPTKs (Syk and Jak2) in neuroinflammation suppression by a bioactive sesquiterpene dimmer (DSF-27). We found that LPS-stimulated BV-2 cells activated Syk and further initiated Akt/NF-κB inflammatory pathway. This Syk-dependent Akt/NF-κB inflammatory pathway can be effectively ameliorated by DSF-27. Moreover, Jak2 was activated by LPS, which was followed by transcriptional factor Stat3 activation. The Jak2/Stat3 signal was suppressed by DSF-27 through inhibition of Jak2 and Stat3 phosphorylation, promotion of Jak/Stat3 inhibitory factors PIAS3 expression, and down-regulation of ERK and p38 MAPK phosphorylation. Furthermore, DSF-27 protected cortical and mesencephalic dopaminergic neurons against neuroinflammatory injury. Taken together, our findings indicate NRPTK signaling pathways including Syk/NF-κB and Jak2/Stat3 cascades are potential anti-neuroinflammatory targets in microglia, and may also set the basis for the use of sesquiterpene dimmer as a therapeutic approach for neuroinflammation via interruption of these pathways. - Highlights: • Sesquiterpene dimmer DSF-27 inhibits inflammatory mediators' production in microglia. • Syk-dependent Akt/NF-κB pathway is important for DSF-27's anti-inflammation activity. • Jak2/Stat3 pathway is important for DSF-27's anti-inflammation activity. • Jak2/Stat3 signaling pathway is partly regulated by ERK and p38 MAPKs and PIAS3. • DSF-27 protects neurons against microglia-mediated neuroinflammatory injury

  19. Simultaneous determination of sesquiterpenes and pyrrolizidine alkaloids from the rhizomes of Petasites hybridus (L.) G.M. et Sch. and dietary supplements using UPLC-UV and HPLC-TOF-MS methods.

    Science.gov (United States)

    Avula, Bharathi; Wang, Yan-Hong; Wang, Mei; Smillie, Troy J; Khan, Ikhlas A

    2012-11-01

    UPLC-UV and HPLC-TOF-MS methods have been developed for the analysis of major sesquiterpenes and pyrrolizidine alkaloids from rhizomes of Petasites hybridus (L.) G.M. et Sch. (Family, Asteracea) and dietary supplements claiming to contain P. hybridus. The best results were obtained with Acquity UPLC™ HSS T3 (100 mm × 2.1 mm, I.D., 1.8 μm) column system using a gradient elution with a mobile phase consisting of ammonium formate (50mM) and acetonitrile (0.05% formic acid) at a constant flow rate of 0.25 mL/min via UPLC-UV. The newly developed method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy and precision. The limits of detection were found to be 5 μg/mL and 0.1 μg/mL for pyrrolizidine alkaloids and sesquiterpenes, respectively by UPLC-UV and 0.001 and 0.01 μg/mL, respectively using HPLC-TOF-MS. The methods were successfully used to analyze different P. hybridus market products, as well as to distinguish between two other Petasites species. The total content of petasins was found to be in the range of 0.02-11.6 mg/dosage form for 15 dietary supplements and no petasins were detected in an additional six dietary supplements. Additionally, pyrrolizidine alkaloids, which are considered to be toxic for the liver, were detected in seven dietary supplements. The amount of petasin in seven dietary supplements was found to be within limits of label claim and no pyrrolizidine alkaloids were detected. HPLC-mass spectrometry coupled with electrospray ionization (ESI) interface method is described for the identification and confirmation of sesquiterpenes and pyrrolizidine alkaloids from plant extracts and dietary supplements that claim to contain P. hybridus as well as different species of Petasites. Copyright © 2012 Elsevier B.V. All rights reserved.

  20. Isolation and structural elucidation of secondary metabolites from plants of the family Flacourtiaceae and Asclepiadaceae, and evaluation of biological activity of the sesquiterpene lactones and the diterpenes of Casearia sp

    International Nuclear Information System (INIS)

    Binns Quiros, Franklin

    2012-01-01

    A phytochemical study was realized of the plants Casearia aculeata, Casearia nitida and Asclepias verticillata, using experiments of nuclear magnetic resonance (NMR) of one and two dimensions. Ten secondary metabolites are isolated from C. aculeata and C. nitida. Three of the secondary metabolites have presented a structure known: a diterpene type kaurane: ent-kaurenic acid, a diterpene type pimarane: oxide of 3β-hydroximanoil and a steroid: 4-stigmastene-3-ona. Seven remaining compounds have corresponded to diterpene type clerodane of novel structure. Eight glycosides of poly oxidized pregnanes of novel structure are isolated from A. verticillata. In vitro tests of cytotoxicity and induction of caspase-3 are performed on leukemia cells type Jurkat T. These tests were performed at fifteen sesquiterpene lactones and at four diterpenes. The tests developed have had the purpose to describe structure-activity relationships that can be linked with the capacity to inhibit the factor NF-κB (sesquiterpene lactones) described in the literature and with the known mechanism of action induction of apoptosis in diterpenes type clerodane. A clear relationship between the capacity (high, intermediate or low) to inhibit the factor NF-κB and the capacity to induce to the caspase-3 has remained without observation in the sesquiterpene lactones. Some structural comparisons related with the cytotoxic capacity and the induction of the caspase-3 have been described for the series of LSs with carbon structure of pseudoguianolides. Diterpenes with carbon structure of diterpenes type clerodane have had greater cytotoxic activity with respect to without carbon structure. Diterpenes type clerodane isolated from the family Flacourtiaceae have been cytotoxics, their capacity to induce to the caspase-3 has remained without be nearby to induction realized by the actinomycin D (pure inducer of the caspase-3). (author) [es

  1. Unidade de Estudos de Mastites em Pequenos Ruminantes - estudos em ovinos de regime extensivo

    OpenAIRE

    Queiroga, Cristina; Potes, Maria Eduarda; Duarte, Elsa Maria; Marinho, Artur; Bettencourt, Carlos Manuel; Matos, Claudino; Belo, Carlos Carmona; Ribeiro, José Manuel; Vilela, Cristina Lobo

    2007-01-01

    A “Unidade de Estudo de Mastites em Pequenos Ruminantes”, formalmente constituída no âmbito do Programa Nacional de Re-equipamento Científico, da Fundação para a Ciência e a Tecnologia, vem dar corpo ao trabalho de colaboração de diversas equipas de investigação que se têm debruçado sobre a problemática das infecções intramamárias em pequenos ruminantes, nos seus aspectos de saúde animal, saúde pública veterinária e qualidade e segurança alimentar. Tem como principal objectivo criar condições...

  2. Panorama de Estudos Linguísticos sobre o Suporte

    Directory of Open Access Journals (Sweden)

    Alex Caldas Simões

    2016-07-01

    Full Text Available Teóricos do campo da linguagem (Cf. MARCUSCHI, 2003, 2008; BEZERRA, 2006, 2007; TÁVORA, 2008 tem indicado que, de alguma forma, o gênero é afetado pelo suporte e vice-versa. Diante dessa questão de pesquisa, nos propomos a resenhar alguns conceitos sobre o suporte em diferentes perspectivas linguísticas, a saber: (a o estudo do suporte na perspectiva textual, conforme postula Marcuschi; (b o estudo do suporte na perspectiva sócio-retórica, conforme postula Bonini e Távora; (c o estudo do suporte na perspectiva da Análise do Discurso, como postula Maingueneau; e (d o estudo do suporte na perspectiva da Linguística Sistêmico- Funcional, conforme propõe Simões (2010. Por fim, concluímos que a categoria teórica de suporte ainda carece de estudos linguísticos, em especial, os aplicados. Ao suporte, apesar das recentes teorizações realizadas, ainda não lhe foi dado um caráter de configuração, assim como se faz com os gêneros do discurso.

  3. Uma ferramenta para planejamento de estudos para concursos

    Directory of Open Access Journals (Sweden)

    Leonardo Pimentel

    2015-11-01

    Full Text Available De acordo com a constituição brasileira, a única forma de se tornar um empregado do governo é passar em um concurso público. O exame de conhecimento do cargo é um dos componentes mais importantes de um concurso. Estudar para esse exame é uma tarefa que requer planejamento. Um meio de obter um bom planejamento é construir uma grade de horários que relacione as matérias a serem estudadas com os respectivos horários disponíveis para o estudo. Esse tipo de problema se assemelha bastante à gerência de projetos, em que se procura estabelecer a distribuição das atividades ao longo do tempo disponível. Assim, torna-se interessante explorar a dinâmica de estudos para concursos como um projeto. O presente trabalho apresenta a ferramenta de apoio ao estudos - Passe-me, baseada no ciclo PDCA (do inglês, Plan-Do-Check-Act. Por se tratar de uma ferramenta web, o tempo de resposta para produzir uma grade de estudos é fundamental. A ferramenta faz uso de algoritmo genético para calcular a grade de horários priorizando a velocidade de resposta. A ferramenta foi avaliada em diferentes cenários de uso e obteve desempenho satisfatório durante a produção da grade de estudos.

  4. Desenvolvimento da Tanatologia: estudos sobre a morte e o morrer

    Directory of Open Access Journals (Sweden)

    Maria Julia Kovács

    2008-12-01

    Full Text Available Este artigo discute os principais temas e pesquisas na área da Tanatologia, estudos sobre a morte e o morrer. São apresentados os autores pioneiros que escreveram as primeiras obras de sistematização da Tanatologia entre os quais: Herman Feifel, Robert Kastenbaum e Elizabeth Kübler-Ross, e os principais temas de estudo: luto, violência e guerra, a morte e a TV, cuidados a pacientes gravemente enfermos, além da formação de profissionais da área de saúde e educação para lidar com pessoas vivendo situações de perdas e morte. São feitas propostas de estudos para o futuro desenvolvimento da Tanatologia em nosso país.

  5. Estudo comparado das sensilas nas antenas de abelha

    OpenAIRE

    Matiello, Cirlei Pereira Guss

    2008-01-01

    Sensilas são as estruturas básicas da percepção dos estímulos ambientais cujas funções mecanorreceptora, quimiorreceptora, termorreceptora e higrorreceptora estão relacionadas à sua morfologia. Abelhas, pelos diferentes graus de sociabilidade e hábitos de forrageamento, são um modelo interessante no estudo da distribuição e tipos de sensilas antenais associadas ao comportamento. Este estudo identificou os tipos de sensilas nas antenas de espécies de abelhas com diferentes graus de sociabilida...

  6. Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi

    Directory of Open Access Journals (Sweden)

    Miguel Peña-Espinoza

    2015-12-01

    Full Text Available The study investigated direct anthelmintic effects of sesquiterpene lactones (SL-containing extracts from forage chicory against free-living and parasitic stages of Ostertagia ostertagi. Freeze-dried leaves from chicory cultivars ‘Spadona’ and ‘Puna II’ were extracted using methanol/water. Total SL were further fractionated by solid-phase extraction and resulting extracts were characterised by high-performance liquid chromatography (HPLC. O. ostertagi eggs from faeces of mono-infected calves were hatched and L1 were used in a larval feeding inhibition assay (LFIA, while cultured L3 were used in a larval exsheathment inhibition assay (LEIA. Adult worms were immediately recovered after slaughter and used for motility inhibition assays (AMIA. Electron microscopy (EM was performed on adult O. ostertagi exposed to 1000 μg extract mL−1 of both chicory cultivars. In all assays, decreasing concentrations of SL-containing extracts in PBS (1% DMSO were tested in replicates with 1% DMSO in PBS as negative controls. HPLC demonstrated similar concentrations of most SL in both extracts. However, Spadona-extract contained significantly higher concentrations of 11, 13-dihydro-8-deoxylactucin (P = 0.01, while Puna II-extract had increased levels of 11, 13-dihydrolactucin (P  0.05. In the AMIA, both SL-containing extracts induced a dose-dependent effect but Spadona-extract showed greater activity and exerted faster worm paralysis than Puna II-extract with significantly lower EC50 (P < 0.0001. No cuticular damage was observed by EM in worms exposed to any of the extracts. We have demonstrated that SL-containing extracts from forage chicory can inhibit feeding of free-living larvae and exert direct effects against parasitic stages of O. ostertagi. Our results may contribute to the identification of natural anti-parasitic compounds and to interpret the in vivo anthelmintic effects of forage chicory.

  7. Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus) show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi.

    Science.gov (United States)

    Peña-Espinoza, Miguel; Boas, Ulrik; Williams, Andrew R; Thamsborg, Stig M; Simonsen, Henrik T; Enemark, Heidi L

    2015-12-01

    The study investigated direct anthelmintic effects of sesquiterpene lactones (SL)-containing extracts from forage chicory against free-living and parasitic stages of Ostertagia ostertagi. Freeze-dried leaves from chicory cultivars 'Spadona' and 'Puna II' were extracted using methanol/water. Total SL were further fractionated by solid-phase extraction and resulting extracts were characterised by high-performance liquid chromatography (HPLC). O. ostertagi eggs from faeces of mono-infected calves were hatched and L1 were used in a larval feeding inhibition assay (LFIA), while cultured L3 were used in a larval exsheathment inhibition assay (LEIA). Adult worms were immediately recovered after slaughter and used for motility inhibition assays (AMIA). Electron microscopy (EM) was performed on adult O. ostertagi exposed to 1000 μg extract mL(-1) of both chicory cultivars. In all assays, decreasing concentrations of SL-containing extracts in PBS (1% DMSO) were tested in replicates with 1% DMSO in PBS as negative controls. HPLC demonstrated similar concentrations of most SL in both extracts. However, Spadona-extract contained significantly higher concentrations of 11, 13-dihydro-8-deoxylactucin (P = 0.01), while Puna II-extract had increased levels of 11, 13-dihydrolactucin (P LFIA, both extracts reduced larval feeding at increasing concentrations, but Spadona-extract showed higher potency confirmed by significantly lower EC50 (P  0.05). In the AMIA, both SL-containing extracts induced a dose-dependent effect but Spadona-extract showed greater activity and exerted faster worm paralysis than Puna II-extract with significantly lower EC50 (P < 0.0001). No cuticular damage was observed by EM in worms exposed to any of the extracts. We have demonstrated that SL-containing extracts from forage chicory can inhibit feeding of free-living larvae and exert direct effects against parasitic stages of O. ostertagi. Our results may contribute to the identification of natural

  8. ACREDITAÇÃO HOSPITALAR: UM ESTUDO DE CASO

    Directory of Open Access Journals (Sweden)

    Felipe Antunes

    2005-06-01

    Full Text Available Este artigo aborda a qualidade e acreditação hospitalar através do Manual das Organizações Prestadoras de Serviços Hospitalares, desenvolvido pela Organização Nacional de Acreditação (ONA. O objetivo geral deste trabalho é avaliar o processo de acreditação, identificando dificuldades e benefícios advindos da implantação deste  Manual. Após a realização de uma revisão bibliográfica sobre qualidade em hospitais e normas da qualidade aplicáveis a hospitais, é feita uma análise crítica teórica do Manual das Organizações Prestadoras de Serviços Hospitalares. Tal análise crítica inclui um estudo comparativo entre os requisitos do Manual e da norma NBR ISO 9001/2000. A seguir, a análise teórica é complementada através de um estudo de caso contemplando a implantação do Manual junto a um hospital de grande porte. Finalmente, são apresentadas as conclusões, baseadas tanto no estudo teórico como no estudo prático.

  9. Pragmatismo e os pragmáticos nos estudos organizacionais

    Directory of Open Access Journals (Sweden)

    Francis Kanashiro Meneghetti

    Full Text Available O objetivo central deste ensaio teórico é avaliar as contribuições do pragmatismo nos Estudos Organizacionais e, os específicos, avaliar se as contribuições teóricas de Charles Peirce e John Dewey possibilitam afirmar o pragmatismo como senso comum, ciência ou teoria do conhecimento; analisar as contribuições - possibilidadese limites - do pragmatismo como conhecimento científico; compreender os percursos do pragmatismo nos Estudos Organizacionais. Chaga-se a conclusão de que ser pragmático é diferente de conceber estudos articulados no campo epistemológico do pragmatismo. O sujeito pragmático vê na sua utilidade e na necessidade deação, o ponto de tensão que coloca em dúvida a veracidade dos pressupostos do pragmatismo como ciência. O que ocorre nos Estudos Organizacionais éa conversão do pragmatismo como conhecimento científico emsubordinação irracional à utilidade e à ação prática, sobretudo, no campo da economia, da política, da ciência, da cultura, da educação, e assim por diante.

  10. Dehydrobrachylaenolide: an eudesmanetype sesquiterpene lactone

    CSIR Research Space (South Africa)

    Rademeyer, M

    2009-01-01

    Full Text Available and trans-annelated with an -methylene -lactone. The absolute structure was assigned by optical rotation measurements compared to those from the synthetic compound with known stereochemistry. The crystal packing is consolidated by C-HO interactions...

  11. Estudos de artes x estudos culturais: o ensino de artes para além dos muros da escola*

    Directory of Open Access Journals (Sweden)

    Edgar Cézar Nolasco

    2010-10-01

    Full Text Available Mediante as discussões acerca das Metodologias de ensino de Artes, nossa Pesquisa Estudos de Artes x Estudos Culturais: o ensino de Artes para além dos muros da escola propõe uma análise crítico-histórica sobre as tendências metodológicas utilizadas para o ensino de Artes nas Escolas, além de apresentar como escopo para tal problemática o que postulam os Estudos Culturais sobre um ensino que faça relações entre e a partir de conceitos como multiculturalidade, educação multicultural, pluralidade ou diversidade cultural. Partindo de uma identificação histórica dos Estudos Culturais, bem como de uma análise sobre as Metodologias do ensino de Artes no Brasil, nossa pesquisa procura fazer tal reflexão crítico-histórica e formular prováveis possibilidades de ganhos reflexivos entre as seguintes disciplinas: Artes e Estudos Culturais. Tais possibilidades foram antes vislumbradas na etapa de observação, vivência e coleta de dados das disciplinas de Estágio Supervisionado I e II, durante a realização do curso de graduação em Artes Visuais – licenciatura – habilitação em Artes Plásticas da UFMS, e acabaram resultando nesta pesquisa, que pretende pensar um ensino de Artes para além dos muros da escola.

  12. Os estudos anatómicos de Leonardo da Vinci

    OpenAIRE

    Ferreira, Ricardo Jorge de Sá

    2015-01-01

    Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas Leonardo da Vinci foi um dos maiores génios de todos os tempos, considerando-se que dentro dos seus variadíssimos estudos realizados, dedicou-se ao estudo da anatomia do corpo humano, com vista a saber a sua constituição e entender o seu funcionamento. Esta dissertação sustenta-se numa revisão bibliográfica, recorrendo ao mét...

  13. Grupo de estudos corpo e cultura de movimento - GEPEC

    Directory of Open Access Journals (Sweden)

    José Pereira de Melo

    2002-01-01

    Full Text Available O presente texto tem o propósito de apresentar a comunidade acadêmica da educação física brasileira, o Grupo de Estudos Corpo e Cultura de Movimento -GEPEC, vinculado ao Departamento de Educação Física da Universidade Federal do Rio Grande do Norte, cuja principal finalidade é desenvolver estudos e pesquisas sobre o corpo e a cultura de movimento, numa interface corn a educação física e a educação. Fundado em 2000 e consolidado pela Pró-reitoria de Pesquisa e Pós-graduação da UFRN em 2001, o GEPEC mostra-se como um campo privilegiado para as reflexões sobre corpo na educação física, bem como para possíveis intervenções na cultura de movimento.

  14. Enfermeiros e clientela com bulimia e anorexia: estudo de caso

    Directory of Open Access Journals (Sweden)

    Claudia Regina Carvalho Martins

    2012-06-01

    Full Text Available Investigação que objetivou identificar a interação de enfermeiros e clientes portadores de bulimia e anorexia. Utilizamos o estudo de caso como recurso, para o qual coletamos dados de prontuários e fizemos entrevistas com oito de quatorze enfermeiros, que foram nossos sujeitos-objeto. De acordo com os enfermeiros, os clientes se isolavam do mundo, vivendo num mundo sem fome e de espelhos que refletiam um corpo sempre acima do "peso ideal"; eram pessoas solitárias, que perderam o brilho pessoal, emitindo sinais de que podiam extinguir a vida deles a qualquer momento. Com o estudo, foi possível identificar a maneira pela qual os enfermeiros interagiam e percebiam os clientes e, em decorrência disso, explicitamos uma prática de cuidar peculiar na enfermagem.

  15. Ana Carolina Escosteguy: Cenários dos estudos culturais

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    Thaisa Cristina Bueno

    2015-12-01

    Full Text Available Autora do livro “Cartografia dos Estudos Culturais”, “Comunicação e recepção” (em coautoria com Nilda Jacks, “Leituras em comunicação, cultura e tecnologia”, além de coautora e organizadora em outras obras de referência sobre o tema cultura e comunicação no Brasil, Ana Carolina Escosteguy é hoje um dos nomes mais importantes quando se pensa em Estudos Culturais no país. Doutora em Ciências da Comunicação pela Universidade de São Paulo (2000, com pós-doutorado no CAMRI (Communication and Media Research Institute, associado ao Department of Journalism and Mass Communication da School of Media, Art and Design da University of Westminster (UK, Ana Carolina Escosteguy é professora titular da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS e bolsista produtividade em pesquisa do CNPq, desde março 2001. Nesta entrevista ela discute pontos sensíveis da área, entre eles o pouco destaque das pesquisas desse campo têm tido no Jornalismo, o fato de a base teórica ser bem mais ampla que os tradicionais estudos de recepção a que são comumente aproximados, não se nega a tratar das confusões com a folkcomunicação, bem como as próprios limites e intersecções desse campo de estudos. Durante esta conversa, aproveita para apontar as obras que considera essenciais para adentrar na área e mostra coragem e segurança para se colocar como uma pesquisadora que busca entender o tempo atual, as tecnologias e as rupturas a partir da perspectiva do usuário. Confiram:

  16. Marketing do mercado de luxo: Estudo de caso Victoria's Secret

    Directory of Open Access Journals (Sweden)

    Ana Francisca Giuliani

    2013-12-01

    Full Text Available Este artigo teve por objetivo identificar quais são as estratégias de marketing utilizadas pela empresa varejista Victoria’s Secret para a fidelização do consumidor de luxo em nível global. O estudo caracterizou-se como uma pesquisa qualitativa, utilizando-se como estratégia de campo o estudo de caso de caráter ilustrativo. Para o alcance do objetivo proposto, dividiu-se o estudo em duas fases. Na primeira, denominada exploratória, buscou-se em dados secundários o embasamento para a construção do referencial teórico. Na segunda, adotou-se a técnica de pesquisa por observação, utilizando-se os sites da Victoria’s Secret, e analisou-se o composto de marketing aplicado ao mercado de luxo com base no modelo proposto por Nahás (2009. Concluiu-se que as estratégias da referida empresa se baseiam no luxo acessível, de modo que, além de conquistar o consumidor, a Victoria’s Secret consegue manter sua fidelidade por apresentar estratégias de forte apelo emocional.

  17. A sociologia funcionalista nos estudos organizacionais: foco em Durkheim

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    Augusto Cabral

    Full Text Available O tema que estrutura este ensaio é o da sociologia funcionalista, particularmente em termos de sua influência nos estudos organizacionais. O seu foco de análise é duplo. Em um primeiro momento, as obras de Durkheim. Em um segundo momento, o impacto e a supremacia dos enfoques funcionalistas nos estudos organizacionais. Embora seus segmentos estejam inter-relacionados, ele é composto de quatro partes. Na primeira, é feita uma análise histórica da emergência e sistematização das ciências sociais, de modo a traçar sua evolução até o surgimento dos trabalhos de Durkheim. Na segunda, tomando como referencial a obra de Burrell e Morgan (1979, é estudado o paradigma funcionalista em termos de sua origem e expoentes, com destaque para Comte e Spencer, visando chegar a uma compreensão inicial do pensamento de Durkheim e de sua sociologia. Na terceira, a vida e as principais obras de Durkheim são sumariadas e discutidas. Por fim, o status atual dos estudos organizacionais -vistos como um conjunto impreciso, elástico e eclético de teorias e pesquisas sociais voltados para os problemas das organizações -é analisado em termos de seus pressupostos epistemológicos e ontológicos subjacentes, como meio de explicitar a supremacia dos enfoques positivistas-funcionalistas.

  18. Estudos organizacionais, (des)colonialidade e estudos da dependência: as contribuições da Cepal

    OpenAIRE

    Wanderley, Sergio

    2015-01-01

    O objetivo deste artigo é (re)visitar o conceito de dependência como categoria de investigação da (e a partir da) América Latina, por meio de uma perspectiva histórica crítica descolonial, a fim de ampliar o espaço de debates em estudos organizacionais e promover alternativas à ordem neoliberal. Este ensaio terá como foco os estudos da dependência realizados pela Comissão Econômica para a América Latina e o Caribe (Cepal) durante a década de 1950 e o início dos anos 1960. A perspectiva descol...

  19. UM ESTUDO SOBRE O SISTEMA DE CONTROLE DE GESTÃO EM EMPRESAS INTERNACIONALIZADAS: estudos de casos

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    Sidmar Roberto Vieira Almeida

    2014-05-01

    Full Text Available O presente estudo se propõe analisar as práticas de controle gerencial de três empresas brasileiras explorando a relação entre os sistemas de controle gerencial destas com o plano de referência extraído da revisão da literatura, buscando evidenciar suas possíveis modificações em função do processo de internacionalização. A metodologia empregada foi a de estudo de casos. Os principais resultados indicam que os impactos da internacionalização nos sistemas de controle gerencial representaram de maneira geral, uma experiência de desafios e aprendizado.

  20. CULTURA ORGANIZACIONAL: UM ESTUDO DE CASO NA EMPRESA MGO

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    Vagner Luís Wentz

    2012-07-01

    Full Text Available O tema cultura tem sido objeto de estudo há muitos anos, e as literaturas existentes apontam uma grande diversidade de conceitos, o que torna as organizações grandes fontes de pesquisa. A MGO, uma multinacional fabricante de produtos agrícolas, convive constantemente com mudanças de seus procedimentos, de suas tarefas do cotidiano e de comportamentos por parte de seus colaboradores, uma relação entre “novatos” e “veteranos” que, em alguns casos, é geradora de conflitos, o que pode vir a prejudicar a imagem da companhia. Diante disso, este estudo tem como objetivo geral analisar a cultura organizacional a partir da percepção das lideranças de uma unidade da empresa MGO situada em Montenegro/RS. Esta pesquisa se classifica, quanto aos procedimentos, como bibliográfica e estudo de caso; quanto aos objetivos, como descritiva; e quanto ao problema, esta pesquisa é classificada como qualitativa. A partir da análise dos dados coletados através de entrevistas semiestruturadas com as lideranças de departamentos diferentes, análise de documentos, observação participante e aplicação do teste para identificação da tipologia de cultura, pode-se identificar a cultura da organização e sugerir algumas melhorias que podem ajudar na harmonia do clima da organização. Palavras-chave: Cultura. Clima. Organização.

  1. Redes e Responsabilidade Social Corporativa: Um Estudo de Caso

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    Adriana Teixeira Bastos

    2015-01-01

    Full Text Available  O presente estudo visa a contribuir para a elucidação de questões relacionadas aos temas responsabilidade social corporativa (RSC e redes de cooperação entre empresas. Com este intento busca-se, através de um estudo de caso, compreender quais os impactos das redes sobre as estratégias de RSC. O estudo de caso em questão está baseado nas ações de Desenvolvimento Regional Sustentável (DRS de uma instituição financeira, que considera esta prática como uma forma dela e de parceiros realizarem suas ações de RSC. O artigo está estruturado considerando-se inicialmente a introdução, a discussão teórica sobre os dois temas que se pretende relacionar (redes e RSC e, após a elucidação sobre a metodologia do trabalho, é apresentada a metodologia do programa DRS da instituição financiadora (Banco Digama e a descrição da aplicação prática do programa para um grupo de parceiros da rede. Dando continuidade, são apresentadas as análises e discussões e, por último, as considerações finais. Entre os principais itens da conclusão, observa-se que os objetivos das organizações, relacionados às RSC, podem ser diferentes entre si, assim como os resultados obtidos, permitindo, inclusive, a coexistência na rede de atores que não alcançaram, na totalidade, seus principais objetivos, embora realizem atividades relacionadas à responsabilidade social. 

  2. Contribuições da fenomenologia para os estudos organizacionais

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    Diego Luiz Teixeira Boava

    Full Text Available Este artigo discute elementos que viabilizam outra possibilidade de compreensão dos fenômenos administrativos, através de uma descrição propedêutica da fenomenologia e do método fenomenológico. Objetiva-se, assim, apresentar as principais contribuições e possibilidades da fenomenologia para os estudos organizacionais. Aprofunda-se em considerações sobre a fenomenologia em geral e os diversos tipos de métodos fenomenológicos em particular, buscando esclarecer sobre a filosofia existente nesses temas, para auxiliar os pesquisadores em seu labor diário. Discorre-se também sobre a redução, que na verdade são duas: a eidética e a fenomenológica. Tais reduções são vitais para retirar o homem de sua atitude natural. Outro assunto aqui abordado é a transposição do método fenomenológico do campo filosófico para o campo da pesquisa empírica. Diante dessa contextualização, tem-se o último assunto a ser abordado, que vai diretamente ao encontro do objetivo desta pesquisa, discutindo-se a utilização da fenomenologia no contexto da administração. A título de ilustração, são apresentados diversos estudos que têm a fenomenologia como tema, bem como um método fenomenológico empírico específico da área de estudos organizacionais. Dessa maneira, a temática neste artigo é situada, sendo preciso agora avançar em direção a outras possibilidades.

  3. Autoconceito em idosos homossexuais: um estudo exploratório

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    Kelly Cristina Santiago Abreu Pereira

    2015-05-01

    Full Text Available Dentro da temática da sexualidade, destaca-se, neste estudo, que a homossexualidade na velhice, apesar de ser tema relevante nos dias atuais, ainda é, porém, pouco explorado na literatura científica; e que não se pode estudar a velhice e a homossexualidade, separando o indivíduo da sociedade em que vive ou da família em que está inserido; dessa forma, deve-se conhecer o individual e o coletivo separadamente, para que se possa realmente compreender esse aspecto na vida de uma pessoa e contribuir para um envelhecimento bem-sucedido.

  4. Dançaterapia no autismo: um estudo de caso

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    Teixeira-Machado, Lavinia

    2015-01-01

    O autismo é uma das desordens neurológicas mais comuns que afetam o desenvolvimento neuropsicomotor de crianças. Intervenções terapêuticas podem intervir nos transtornos de comunicação e nos comportamentos estereotipados, desse modo a dança, como terapia, pode ativar vias sensoriais que viabilizam o aprimoramento do gesto. Este estudo visou observar os efeitos da dançaterapia no desempenho motor e gestual, no equilíbrio corporal e na marcha, bem como na qualidade de vida de um adolescente com...

  5. Marketing educacional: um estudo comparativo das atividades de marketing

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    Marta Maria Cunha Carneiro

    2015-01-01

    A revisão teórica apontou uma visão geral dos temas de marketing e educação por meio da cronologia histórica, da discussão conceitual, da relevância teórica e prática de cada assunto, dos principais desafios para pesquisa e o desenvolvimento do tema marketing educacional. Identificou-se e discutiu-se as atividades de marketing desenvolvidas nas instituições de ensino superior à luz dos fundamentos teóricos. A metodologia abordou os aspectos relacionados ao protocolo do estudo de caso e uma pe...

  6. Estudo de estabilidade do antibiótico meropenem

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    Andreas Sebastian Loureiro Mendez

    2007-01-01

    A estabilidade de medicamentos constitui um tema atual e de grande importância no meio científico. Tem sido constantemente abordada em estudos direcionados à avaliação da qualidade das preparações farmacêuticas. O meropenem, um antibiótico carbapenêmico de amplo espectro de ação, é utilizado como um importante recurso terapêutico para o tratamento de infecções graves. Por ser um agente antimicrobiano ativo e eficaz, de uso clínico em muitos países, tornase necessária a pesquisa mais aprofunda...

  7. Encefalopatia hepática: estudo de 50 casos

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    Luiz Antonio de Lima Resende

    1984-12-01

    Full Text Available Os autores apresentam estudo retrospectivo de 50 pacientes com encefalopatia hepática atendidos no Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto a partir de 1959 e discutem os aspectos clínicos, laboratoriais e anátomo-patológicos. Expõem os diagnósticos das hepatopatias, os fatores precipitantes dos episódios de coma e pré-coma hepático e enfatizam o papel da gasometria arterial no estabelecimento do diagnóstico.

  8. Abandono em Psicoterapia Psicanalítica : estudo qualitativo

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    Simone Isabel Jung

    2013-01-01

    Esta tese teve como objetivo geral analisar o fenômeno do abandono em psicoterapia psicanalítica (PP) através de metodologia qualitativa. Para tanto, três artigos foram realizados identificando características de inicio e término de tratamento de pacientes adultos classificados por seus psicoterapeutas como pacientes que abandonaram a PP, em um serviço de atendimento da cidade de Porto Alegre/Brasil, cujo objetivo principal é a formação de especialistas em PP. Foi utilizado em todos os estudo...

  9. Estudo de desempenho do aquecedor solar de baixo custo

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    Siqueira, Débora Abrahão

    2009-01-01

    Uma das alternativas para diminuir o consumo de energia elétrica para aquecimento de água será popularizar o uso da energia solar. Este trabalho vem a contribuir com estudos sobre o Aquecedor Solar de Baixo Custo, ASBC, que é um novo conceito de aquecedor solar de água para residência, todo feito de materiais poliméricos, com investimento relativamente pequeno e que pode ser construído manualmente. A placa do coletor, que absorve a energia solar e a transfere para a água na for...

  10. Obesidade e Saúde Oral Infantil: estudo piloto

    OpenAIRE

    Fidalgo, Ana Marta Saraiva Gouveia

    2016-01-01

    Trabalho final do 5º ano com vista à atribuição do grau de mestre no âmbito do ciclo de estudos de Mestrado Integrado em Medicina Dentária apresentado à Faculdade de Medicina da Universidade de Coimbra. Introdução: A obesidade infantil (OI) é uma epidemia emergente com consequências psicossociais e físicas, incluindo risco de desenvolvimento de patologias metabólicas, cardiovasculares, músculo-esqueléticas e respiratórias. A relação potencial entre a OI e a patologia oral tem sido crescent...

  11. "Buzz marketing" : estudo de caso da marca Red Bull

    OpenAIRE

    Conceição, Miguel David

    2012-01-01

    Este relatório é baseado no estágio efetuado na agência de publicidade Ogilvy & Mather e é dedicado à temática do Buzz Marketing. De modo a poder explorar a fundo as dinâmicas deste tema foi feita uma análise à estratégia de marketing da marca Red Bull, considerada como um dos principais estudos de caso no âmbito do Buzz Marketing. Este tema pode ser definido como uma estratégia de marketing inovadora cujo objetivo principal é a criação de conversas entre os consumidores, colocando-os a falar...

  12. Perspectivas psicossociais para o estudo do cotidiano de trabalho

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    Fábio de Oliveira

    2014-04-01

    Full Text Available Este estudo teve por objetivo analisar o debate e o aprofundamento teórico e metodológico desenvolvido nos estudos sobre a vida cotidiana no trabalho. A investigação partiu do diálogo interdisciplinar entre a antropologia do trabalho, a ergologia e a psicologia social do trabalho e sistematizou as contribuições mútuas entre esses campos científicos, suas aproximações e seus afastamentos da tematização e da apropriação da atividade humana no trabalho sob a perspectiva do cotidiano. A análise comparativa destacou eixos que diferenciam as formas de aproximação ao objeto, que se referem às relações estabelecidas entre saberes práticos e técnico-científicos e aos modos de intervenção sobre as situações de trabalho. As abordagens comparativas compartilham o interesse em conhecer o trabalho real, o valor conferido aos conhecimentos dos trabalhadores e a sensibilidade às dimensões micropolíticas do cotidiano. Conclui-se que a localização em cada um desses eixos depende da maior ou menor filiação às tradições da antropologia ou da ergonomia.

  13. GESTÃO DE MARCAS: UM ESTUDO SOBRE A UNIMED

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    Hellen Taís Zanóbio

    2018-03-01

    Full Text Available O crescimento da demanda por serviços de saúde suplementar somado às mudanças da relação marca versus consumidor tornam esse mercado cada vez mais competitivo. Esses fatores implicam na movimentação das prestadoras deste tipo de serviço em relação à necessidade de identificar e implantar a melhor forma de gestão de marca, com o objetivo de torná-la conhecida e diferenciada. A empresa estudada foi a Unimed, a maior Cooperativa de Trabalho Médico do Brasil, onde se aplica o seguinte problema de pesquisa: quais as estratégias utilizadas pela Unimed na gestão de sua marca? Este estudo tem o objetivo de estudar as estratégias aplicadas pela Unimed em estudos que as exploram utilizando a metodologia de pesquisa de Revisão Bibliográfica. Os resultados obtidos foram o destaque da importância dada pela Unimed à gestão de sua marca onde a centraliza como diferencial competitivo, e o sucesso do uso das estratégias de gestão de marca como a identidade, imagem, personalidade, arquitetura e valor, tornando-a uma marca forte e consolidada no mercado.

  14. Estudos in vitro e in vivo em peixes da Ria de Aveiro

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    Ferreira, Carla Sofia dos Santos

    2007-01-01

    Dado a importância e complementaridade existente entre estudos de campo e estudos de laboratório, o presente trabalho engloba ambos os tipos de estudo. Os peixes desempenham um papel ecológico bastante relevante ao nível das cadeias tróficas aquáticas, pelo que são frequentemente usados como organismos sentinela em estudos de biomonitorização. Assim, para o presente trabalho, foram adoptadas duas espécies representativas da ictiofauna da Ria de Aveiro: a tainha garrento (Liz...

  15. Sintomas depressivos em crianças: estudos com duas versões do CDI

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    Miriam Cruvinel

    Full Text Available O objetivo deste estudo foi identificar o número de crianças com sintomas depressivos em duas amostras de estudantes da cidade de campinas. Utilizou-se, no estudo 1, a versão brasileira do Inventário de Depressão Infantil (CDI e, no estudo 2, a versão original. Do estudo 1, realizado em 2001, participaram 169 estudantes de 3ª, 4ª e 5ª séries, e, do estudo 2, conduzido em 2005, 157 alunos de 3ª e 4ª séries. A prevalência de sintomatologia depressiva no estudo 1 foi de 3,55%, e, no estudo 2, de 17%. Em ambos os estudos, não foram encontradas diferenças significativas entre a média no CDI em relação às variáveis demográficas. conclui-se pela necessidade de mais estudos nacionais de prevalência no ensino fundamental e de esforços direcionados não só para a identificação dos fatores de risco, de proteção e das variáveis associadas mas também do refinamento dos instrumentos de medidas da depressão infantil.

  16. Medidas de rede e apoio social no Estudo Pró-Saúde: pré-testes e estudo piloto

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    Dóra Chor

    Full Text Available Neste artigo, relatamos a metodologia de seleção de perguntas sobre rede e apoio social, incluídas em um estudo de coorte de 4.030 funcionários de uma universidade pública no Rio de Janeiro. Em primeiro lugar, a adequação de conceitos foi explorada em discussões de grupos de voluntários. Em seguida, o questionário do Medical Outcomes Study foi submetido a procedimentos padronizados de tradução e versão. As perguntas foram a seguir avaliadas em cinco etapas de pré-testes e estudo piloto. Nenhuma pergunta apresentou proporção de não-resposta acima de 5%. Os coeficientes de correlação de Pearson entre os itens foram distantes de zero e da unidade; a correlação entre cada item e o escore de sua dimensão foi superior a 0,80 em quase todos os casos. Finalmente, os coeficientes Alpha de Cronbach foram superiores a 0,70 em todas as dimensões. Os resultados sugerem que aspectos de rede e apoio social serão mensurados adequadamente, permitindo a investigação de suas associações com desenlaces relacionados à saúde em um grupo populacional no Brasil.

  17. Otomycosis: a retrospective study Otomicoses: um estudo retrospectivo

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    Zélia Braz Vieira da Silva Pontes

    2009-06-01

    Full Text Available Otomycosis is a fungal infection of the external ear canal with only a few studies about its real frequence in Brazil. AIM: to evaluate otomycosis frequence and characteristics in patients with clinical suspicion of external otitis. STUDY DESIGN: Retrospective study with transversal cohort (2000-2006. MATERIALS AND METHODS:103 patients were assigned to mycological diagnosis (direct microscopic examination and culture. RESULTS: Otomycosis was diagnosed in 19.4% of the patients. Patient age varied from 2 to 66 years (an average of 23.5 years of age, and 60% of otomycosis cases were seen in women between 2 to 20 years of age. Chronic otitis, previous antibiotic therapy and the lack of cerumen were predisposing factors; itching, otalgia, otorrhea and hypoacusis were the symptoms reported by the patients. The most frequently isolated species were C. albicans (30%, C. parapsilosis (20%, A. niger (20%, A. flavus (10%, A. fumigatus (5%, C. tropicalis (5%, Trichosporon asahii (5% and Scedosporium apiospermum (5%. CONCLUSIONS: Otomycosis is endemic in João Pessoa-PB. Clinical exam and mycological studies are important for diagnostic purposes because otomycosis symptoms are not specific.Otomicose é uma infecção fúngica do conduto auditivo externo com poucos estudos sobre sua real frequência no Brasil. OBJETIVO: Avaliar a frequência e características das otomicoses em pacientes com suspeita clínica de otite externa. DESENHO DO ESTUDO: Estudo retrospectivo com corte transversal (2000-2006. MATERIAL E MÉTODOS: 103 pacientes foram atendidos para diagnóstico micológico (exame microscópico direto e cultivo. RESULTADOS: Otomicoses foram diagnosticadas em 19,4% dos pacientes. A idade desses pacientes variou de 2 a 66 anos (média de 23,5 anos e 60% das otomicoses foram observadas em mulheres entre 2 a 20 anos de idade. Otite crônica, antibioticoterapia prévia e ausência de cerume foram os fatores predisponentes e prurido otológico, otalgia

  18. História e oralidade nos estudos comunicacionais

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    Juniele Rabêlo Almeida

    2016-05-01

    Full Text Available Concepções, métodos e formas do conhecimento histórico são expressos em diversos estudos na área de comunicação social. Buscamos analisar, na interface história e narrativa oral, aspectos da chamada mediação cultural, na qual a comunicação está para além da condição de canal. Acreditamos que os estudos comunicacionais não recaem, necessariamente, sobre especificidades técnicas, mas sobre a posição que a comunicação assume no campo histórico-cultural. Interações, expressas nos trabalhos de história oral, se configuram (e resultam em processos comunicacionais que indicam construções sócio históricas e diferentes referenciais de pertencimento. Palavras-chave: Estudos comunicacionais, História, Narrativas Orais.   Abstract: Conceptions, methods and historical knowledge forms are expressed in several studies in the area of social communication. We analyze, history and oral narrative interface, called aspects of cultural mediation, in which the communication is in addition to the channel condition. We believe that communication studies do not fall necessarily on technicalities, but on the position that the communication is in the historical-cultural field. Interactions, expressed in the works of oral history, are configured (and result in communication processes that indicate historical social constructions and different frames of belonging. Keywords: communication Studies, History, Oral Narratives.   Resumen: Conceptos, métodos y formas de conocimiento histórico se expresan en varios estudios en el área de la comunicación social. Analizamos, la historia y la interfaz de la narrativa oral, llamados aspectos de mediación cultural, en el que la comunicación es, además de la condición de canal. Creemos que los estudios de comunicación no caen necesariamente en técnicos, sino a la posición de que la comunicación es en el campo histórico-cultural. Interacciones, expresados en las obras de la historia oral, est

  19. Estudos de Comunidade e ciências sociais no Brasil

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    Nemuel da Silva Oliveira

    2011-12-01

    Full Text Available O objetivo deste artigo é fazer um balanço da literatura sobre os Estudos de Comunidade (EC no Brasil, destacando o contexto intelectual em que foram produzidos e os debates que suscitaram, especialmente entre as décadas de 1940 e 1960. Método de pesquisa em comunidades, oriundo da Antropologia, em investigações sobre o processo de mudança social, os EC estiveram em voga nos Estados Unidos entre os anos 1920 e 1950, tendo papel fundamental na institucionalização das Ciências Sociais no Brasil. Destacamos três aspectos na produção desses estudos no país, iniciando com uma abordagem conceitual: definições, origem e sua relação com temáticas marcantes nos anos 1950. Em seguida, privilegiamos os aspectos relacionados ao papel dos EC na história das Ciências Sociais no Brasil, com destaque para o processo de institucionalização das Ciências Sociais. Por fim, revisitamos os debates que tais estudos geraram entre os cientistas sociais quanto ao padrão de trabalho sociológico a ser desenvolvido naquele momento.The purpose of this article is to examine the literature about Community Studies(CS in Brazil, highlighting their intellectual context of production and the debates evoked by them, especially between the 1940's and 1960's. As a research method arising from Anthropology on communities in social change process, CS was used in United States between 1920's and 1950's, and played a fundamental role in the institutionalization of Social Sciences in Brazil. Three aspects are highlighted on CS production: first, in a conceptual approach, we discuss their definitions, origins and relation to main topics in the 1950's. The second aspect is related to the role of CS in the history of Social Sciences, especially their institutionalization process. By the end, we revisit the debates between social scientists about the sociological work standard to be developed at that time.

  20. Estudos sobre a toxicologia da ε-caprolactama

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    Marcus Vinicius Justo Bomfim

    2009-03-01

    Full Text Available A ε-caprolactama (CAP é um monômero precursor de polímeros denominados nylon 6. Esses polímeros destinam-se à produção de tapetes, vestuário e materiais plásticos tais como equipamentos, sistemas e componentes automotivos, conectores, além de embalagens plásticas. Resíduos de CAP podem migrar de embalagens plásticas de nylon 6 para os alimentos. Diante disso, foi de interesse realizar uma revisão dos efeitos relativos à exposição à CAP e o seu impacto sobre a saúde humana. Estudos epidemiológicos indicam a possibilidade da CAP causar inflamações oculares e cutâneas, além de irritações no sistema respiratório. Pode ocorrer ainda hipotensão, taquicardia, palpitações, rinorréia, ressecamento nasal, efeitos geniturinários e sobre a reprodução como distúrbios nas funções menstrual e ovariana, e complicações no parto; além de problemas neurológicos e hematológicos. Estudos com animais são consistentes com tais relatos. Os estudos de genotoxicidade in vitro e in vivo por via oral e intraperitoneal mostram em sua grande maioria, resultados negativos, bem como ausência de efeitos carcinogênicos em ratos e camundongos e sobre o desenvolvimento e reprodução em ratos e coelhos.ε -Caprolactam (CAP is a precursor monomer of nylon 6 polymers. Nylon 6 is used in the manufacture of carpets, clothes and plastic materials, such as equipment, systems and automotive components, connectors and plastic packaging. CAP residues can migrate from nylon 6 plastic packaging to foods. Given this fact, this review was realized concerning the effects of CAP exposure and its impact on human health. Epidemiological studies indicate that CAP could cause ocular, cutaneous and respiratory irritations, as well as hypotension, tachycardia, palpitations, rhinorrhea, nose dryness, neurological and blood problems, and genitourinary and reproductive effects, such as alterations in ovarian-menstrual functions and pregnancy

  1. Estudo sociocultural alimentício dos alunos da UNILA

    OpenAIRE

    Arcain, Beatriz Mitidiero Stachissini; Reyes, Patricio David; Perez, Cecilio Luis Correa; Campos, Sandra Regina Carneiro de; Brito, Gleisson Alisson Pereira de; Furtado, Danúbia Frasson

    2015-01-01

    Anais do IV Encontro de Iniciação Científica da Unila - “UNILA 5 anos: Integração em Ciência, Tecnologia e Cultura na Tríplice Fronteira” - 05 e 06 de novembro de 2015 – Sessão Ciências Biológicas e Saúde Coletiva : Alterações ambientais e comportamentais podem causar interferências no peso através de alterações da massa adiposa, ocasionadas por modificações dos hábitos alimentares. As modificações do ambiente geram um estresse no indivíduo sendo que, segundo estudos, há diferente...

  2. Estudo da fonoforese de ciclofenaco dietilamonio em voluntarios sadios

    OpenAIRE

    Claudio Fusaro

    2006-01-01

    Resumo: O transporte transdérmico de substâncias com o ultra-som terapêutico, método denominado fonoforese, oferece muitas vantagens quando comparado as outras vias de administração de drogas. O objetivo deste estudo foi verificar a influência do ultra-som na absorção de diclofenaco dietilamônio (Cataflam Emulgel®, Novartis) após fonoforese em voluntários sadios. Doze voluntários sadios foram submetidos à aplicação tópica de Cataflam Emulgel® em uma área de 150cm2 na região do dorso. No grupo...

  3. CAPITAL INTELECTUAL COMO VANTAGEM COMPETITIVA: UM ESTUDO BIBLIOGRÁFICO

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    Fabiano Greter Moreira

    2015-01-01

    Full Text Available Opresente artigo tem o objetivo de contribuir para o entendimento da importânciado capital intelectual como vantagem competitiva para o sucesso das empresas,por meio de sua gestão estratégica, em seu repertório de práticasadministrativas e desenvolver seu potencial inovador, representados pelasdiferentes formas de conhecimento. Neste sentido, obteve como método um estudode caráter exploratório, com o intuito de conceituar o capital intelectual,como vantagem competitiva e sustentável para as organizações. Foram apresentados diversos estudos que demonstram como oconhecimento é considerado uma das principais fontes essenciais para avitalidade das organizações e a competitividade dos negócios.

  4. Interdisciplinaridade nos Estudos de Tradução

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    José Lambert

    2017-05-01

    Full Text Available http://dx.doi.org/10.5007/2175-7968.2017v37n2p246 Desde 1992 (Snell-Hornby et al. 1994 os Estudos de Tradução* (doravante EdT reivindicaram o estatuto de  “interdisciplina”.  Aliás, ainda há dúvidas sobre seu estatuto acadêmico que é mais recente em relação à profissão. Desde Holmes 1988 [1972], o desenvolvimento dos EdT foi, várias vezes, considerado uma história de sucesso; embora não se saiba claramente se os resultados acadêmicos (argumentos e componentes peculiares vinculados ao seu prestígio, tais como livros, sociedades, estudiosos reconhecidos, congressos, foram mais decisivos do que o mercado da tradução no progressivo reconhecimento dos EdT.

  5. A Fluvialidade no Rio Pinheiros: um projeto de estudo

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    Jorge Hajime Oseki

    2000-12-01

    Full Text Available Este texto tratava originariamente de um projeto de estudos e reconstitui a trajetória teórica que representou a mudança da problemática da produção do ambiente construído para aquela da produção do espaço - na concepção lefebvriana - cujo conceito e exposição engloba além da crítica ao econômico, uma crítica ao político, ao cultural, ao cotidiano e ao urbano podendo, portanto, ser útil para esclarecer a dimensão natural e social do espaço e da paisagem

  6. Planeamento de uma estrada utilizando CCS Candy - Caso de estudo

    OpenAIRE

    Oliveira, Sara Maria de

    2013-01-01

    O tema desta dissertação foi escolhido tendo em consideração a crescente necessidade de planeamento de empreitadas de construção de estradas, sendo um dos objetivos desta dissertação o de oferecer uma visão global do processo de planeamento da construção de uma estrada, focado apenas no fator tempo, recorrendo a ferramentas informáticas e utilizando um caso de estudo de uma estrada real e já construída. Inicialmente, faz-se uma breve descrição dos métodos de planeamento mais aplicados em p...

  7. Estudo das ausências da equipe de enfermagem

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    Janaina Haidê Rodrigues Belem

    1998-12-01

    Full Text Available As ausências dos elementos da equipe de enfermagem comprometem a organização do trabalho causando sobrecarga dessa equipe, e queda na qualidade da assistência de enfermagem. O presente estudo caracteriza o tipo e distribuição das ausências da equipe de enfermagem em um hospital geral de grande porte, propondo um índice de Segurança Técnica para cobertura das ausências a partir da realidade investigada. Foram estudadas 8 tipos de ausências entre previstas e não previstas. O maior número de tipos de ausências encontradas foram aquelas referentes à ausências previstas, em seqüência encontramos licença maternidade representando o maior número de ausências não previstas.

  8. Mobilidade e affordance como cerne dos Estudos Pessoa-Ambiente

    OpenAIRE

    Günther,Hartmut

    2003-01-01

    Partindo de uma reflexão sobre a multiplicidade disciplinar, teórica, metodológica e terminológica e utilizando uma analogia com o modelo multivariado da correlação canônica, propõe-se o rótulo Estudos Pessoa-Ambiente para englobar as diferentes sub-áreas disciplinares e temáticas que lidam com as relações recíprocas pessoa-espaço físico. Segue uma discussão de quatro conceitos do espaço (espaço pessoal, territorialidade, apinhamento e privacidade) e sua relação com mobilidade. Após algumas c...

  9. Estudo FitoquÃmico de Pilocarpus sulcatus Skorupa (Rutaceae)

    OpenAIRE

    AntÃnio HonÃrio de Sousa

    2009-01-01

    O presente trabalho descreve o estudo fitoquÃmico de Pilocarpus sulcatus Skorupa e o levantamento acerca dos metabÃlitos secundÃrios jà isolados do tÃxon Pilocarpus. O trabalho envolveu a extraÃÃo e identificaÃÃo dos constituintes volÃteis das folhas, casca do caule, lenho do caule, cascas das raÃzes e lenho das raÃzes alÃm do isolamento e determinaÃÃo estrutural dos constituintes fixos das folhas e lenho do caule de P. sulcatus. Trata-se de uma espÃcie com descriÃÃo botÃnica recente, ainda s...

  10. (Sem saber e (com poder nos estudos organizacionais

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    José Henrique de Faria

    Full Text Available Bacon afirma que saber é poder. Tragtenberg contesta. Essa discussão, na contemporaneidade, faz-se mais importante do que se possa imaginar. Por isso, o objetivo central deste artigo é verificar as relações entre saber e poder, na atualidade, levando em consideração o papel da ciência e dos elementos imediatos a ela relacionados. Quanto aos objetivos específicos deste estudo, destacam-se: · compreender o sentido da filosofia e da ciência e suas relações com a ideologia; · verificar como o discurso da neutralidade axiológica da ciência se apresenta como mito da modernidade e como se dá a presença da "fé" na filosofia e na ciência, na contemporaneidade; · refletir sobre a consolidação da ciência como força produtiva e/ou como mercadoria no atual sistema econômico; · destacar a importância do complexo industrial militar como financiador de grande parte dos atuais estudos científicos; · entender o processo de racionalização, avaliando a importância do pragmatismo e da burocracia universitária como afirmação da ciência na atualidade. O texto conclui que tanto é possível a existência de saber como poder (Bacon como a de não saber, mas com poder (Tragtenberg para compreendermos a relação entre saber e poder.

  11. Estudo bacteriológico de lesões periapicais

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    ROCHA Márcia Maria de Negreiros Pinto

    1998-01-01

    Full Text Available O tecido perirradicular de 30 casos cirúrgicos foi submetido a exame microbiológico e histopatológico. Foram isoladas 137 cepas bacterianas das 30 lesões periapicais estudadas. Do total de bactérias isoladas, 90 (65,7% foram caracterizadas como anaeróbias estritas, 40 (29,2% como anaeróbias facultativas e 7 (5,1% como aeróbias estritas. Fusobacterium nucleatum, bacilos Gram-negativos pigmentados anaeróbios estritos, Peptostreptococcus sp, Streptococcus mitis e bacilos Gram-positivos não esporulados anaeróbios estritos foram, em ordem decrescente de freqüência, as bactérias mais comumente isoladas das lesões periapicais. A análise histopatológica demonstrou prevalência de granuloma periapical. O estudo de sensibilidade a antimicrobianos foi realizado em 16 cepas de Fusobacterium nucleatum. Os antimicrobianos utilizados foram penicilina, cefoxitina, eritromicina, metronidazol e tetraciclina. Todas as amostras apresentaram suscetibilidade à cefoxitina, tetraciclina e metronidazol; os percentuais de resistência à penicilina e à eritromicina foram 12,5 e 68,8, respectivamente. Os percentuais de resistência de Fusobacterium nucleatum, uma espécie bacteriana importante na patogênese das lesões periapicais, à penicilina e à eritromicina, enfatizam a necessidade de outros estudos de resistência a antimicrobianos de bactérias isoladas em infecções odontogênicas.

  12. AS RELAÇÕES DE PODER EM ESTUDO OBSERVACIONAL

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    Rafael Fernandes de Mesquita

    2016-01-01

    Full Text Available Este trabalho objetiva analisar como se desenvolvem as relações de poder no filme “Django Livre” (2013. Assim, atende-se a este objetivo a partir de um estudo observacional, de abordagem metodológica qualitativa, em linguagem fílmica com observador não participante, e caráter teórico-empírico. Toma-se por base, principalmente, a abordagem sobre poder de Weber (1991, Lukes (1980, Foucault (2012 e Hard e Clegg (2001, destacando que as relações sociais estão permeadas por lutas pelo poder, sobretudo a dominação tradicional, o cerceamento e a disciplina. O tema foi escolhido a partir do filme “Django Livre”, em que a escravatura é palco para o estudo das relações de poder naquela sociedade. Considera-se, finalmente, que o poder se manifesta, principalmente, através da tradição e poder econômico. A obediência não era a um estatuto, mas sim pessoal ao senhor. As relações estabelecidas se davam através do costume e que, ao tentar mudar a dinâmica do sistema, Django sofre violência física e a liberdade acontece diante da não mais aceitação daquela situação de disciplina e obediência.

  13. Estudos cronotópicos em narrativas audiovisuais

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    Egle Müller Spinelli

    2008-11-01

    Full Text Available O presente trabalho pretende aplicar o conceito de cronotopia de Bakhtin à análise de narrativas audiovisuais. Para tanto, será discutido o conceito de cronotopo bakhtiniano e sua relação com os estudos de Saussure, com o intuito de mostrar como os elementos cronotópicos podem estar ligados a contextos que vão além do enunciado, remetendo a mecanismos da enunciação. No estudo são analisados filmes que apresentam determinadas características semelhantes às dos principais tipos de cronotopia estabelecidos por Bakhtin: a da aventura, a do cotidiano e a autobiográfica, respectivamente correspondentes aos filmes Corra Lola, Corra (Tom Tykwer, 1998, Boca de Ouro (Nelson Pereira dos Santos, 1962 e Rashomon (Akira Kurosawa, 1950. Palavras-chave: cronotopia; enunciação; cinema; Bakhtin Abstract Chronotopic studies in audiovisual narratives - This paper proposes to apply Bakhtin's concept of chronotopia in an analysis of audiovisual discourse. To this end, it discusses the Bakhtin's postulation of the chronotope and its relation with the studies of Saussure in order to show how chronotopic elements may be connected to contexts that go beyond simple statements, suggesting mechanisms of enunciation. The films Run Lola, Run (Tom Tykwer, 1998, Boca de Ouro (Nelson Pereira dos Santos, 1962 and Rashomon (Akira Kurosawa, 1950 are used here to illustrate the principal chronotopic types established by Bakhtin, namely, adventure, everyday life and autobiography. Key words: chronotope; enunciation; cinema; Bakhtin

  14. O Zulliger no sistema compreensivo: um estudo de fidedignidade

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    Anna Elisa de Villemor-Amaral

    Full Text Available Os testes psicológicos têm passado por verificações criteriosas de validade e precisão a fim de atender às exigências éticas e científicas. O objetivo desta pesquisa foi averiguar a precisão do Teste Zulliger no sistema compreensivo por meio do teste-reteste. Participaram do estudo 25 sujeitos, do sexo masculino, não pacientes, estudantes de teologia do interior de São Paulo. Utilizou-se um questionário para dados socioeconômico-familiar e o Zulliger. A aplicação dos instrumentos foi individual, durando cerca de 40 minutos, com intervalo de 5 meses entre teste-reteste. Dos protocolos, 25% foram recodificados por um juiz às cegas, obtendo-se índices satisfatórios de precisão. Foram selecionados 16 indicadores para se realizar as estatísticas descritivas e estudos de correlação com método de Pearson. Desses, dez alcançaram índices de precisão satisfatórios, entre 0,60-0,99; quatro tiveram uma precisão entre 0,40-0,60, todos com nível de significância entre 0,01 e 0,05. Os resultados foram satisfatórios e contribuíram para demonstrar a precisão do instrumento.

  15. VIDA COTIDIANA, AFETIVIDADE E ESQUIZOFRENIA: UM ESTUDO EM ADULTOS PORTUGUESES

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    Teresa Freire

    2014-12-01

    Full Text Available O presente estudo, realizado em Portugal, teve como objetivo analisar as experiências da vida diária de pessoas com diagnóstico de esquizofrenia, com particular ênfase na dimensão afetiva da experiência. Recorreu-se ao Experience Sampling Method (ESM para recolha de dados relativos à experiência diária ao longo de uma semana. Participaram neste estudo 14 pessoas com pelos menos três anos de diagnóstico de esquizofrenia, estáveis do ponto de vista médico, a viver em contexto extra-hospitalar e alvo de projetos de reabilitação profissional. Os resultados indicam que os participantes passam grande parte do tempo em casa, sozinhos ou com a família, a realizar atividades de tempo livre. Os aspectos afetivos positivos (emoções e sentimentos predominam na sua vida diária, porém esta situação se altera quando os indivíduos estão sozinhos, havendo um decréscimo acentuado da afetividade positiva e um aumento significativo da afetividade negativa. Estes resultados reforçam a perspectiva de integração comunitária e a necessidade de esta ser acompanhada da criação de estruturas comunitárias que promovam atividades integradas de reabilitação, estruturadas e promotoras do desenvolvimento e das interações sociais.

  16. The Chemopreventive Effect of Tanacetum Polycephalum Against LA7-Induced Breast Cancer in Rats and the Apoptotic Effect of a Cytotoxic Sesquiterpene Lactone in MCF7 Cells: A Bioassay-Guided Approach

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    Hamed Karimian

    2015-06-01

    Full Text Available Background: Tanacetum polycephalum L. Schultz-Bip is a member of the Asteraceae family. This study evaluated the chemopreventive effect of a T. polycephalum hexane extract (TPHE using in in vivo and in vitro models. Methods and Results: Five groups of rats: normal control, cancer control, TPHE low dose, TPHE high dose and positive control (tamoxifen were used for the in vivo study. Histopathological examination showed that TPHE significantly suppressed the carcinogenic effect of LA7 tumour cells. The tumour sections from TPHE-treated rats demonstrated significantly reduced expression of Ki67 and PCNA compared to the cancer control group. Using a bioassay-guided approach, the cytotoxic compound of TPHE was identified as a tricyclic sesquiterpene lactone, namely, 8β- hydroxyl- 4β, 15- dihydrozaluzanin C (HDZC. Signs of early and late apoptosis were observed in MCF7 cells treated with HDZC and were attributed to the mitochondrial intrinsic pathway based on the up-regulation of Bax and the down-regulation of Bcl-2. HDZC induced cell cycle arrest in MCF7 cells and increased the expression of p21 and p27 at the mRNA and protein levels. Conclusion: This results of this study substantiate the anticancer effect of TPHE and highlight the involvement of HDZC as one of the contributing compounds that act by initiating mitochondrial-mediated apoptosis.

  17. The Chemopreventive Effect of Tanacetum Polycephalum Against LA7-Induced Breast Cancer in Rats and the Apoptotic Effect of a Cytotoxic Sesquiterpene Lactone in MCF7 Cells: A Bioassay-Guided Approach.

    Science.gov (United States)

    Karimian, Hamed; Fadaeinasab, Mehran; Moghadamtousi, Soheil Zorofchian; Hajrezaei, Maryam; Zahedifard, Maryam; Razavi, Mahboubeh; Safi, Sher Zaman; Mohan, Syam; Khalifa, Shaden A M; El-Seedi, Hesham R; Abdulla, Mahmood Amin; Ali, Hapipah Mohd; Noordin, Mohamad Ibrahim

    2015-01-01

    Tanacetum polycephalum L. Schultz-Bip is a member of the Asteraceae family. This study evaluated the chemopreventive effect of a T. polycephalum hexane extract (TPHE) using in in vivo and in vitro models. Five groups of rats: normal control, cancer control, TPHE low dose, TPHE high dose and positive control (tamoxifen) were used for the in vivo study. Histopathological examination showed that TPHE significantly suppressed the carcinogenic effect of LA7 tumour cells. The tumour sections from TPHE-treated rats demonstrated significantly reduced expression of Ki67 and PCNA compared to the cancer control group. Using a bioassay-guided approach, the cytotoxic compound of TPHE was identified as a tricyclic sesquiterpene lactone, namely, 8β- hydroxyl- 4β, 15- dihydrozaluzanin C (HDZC). Signs of early and late apoptosis were observed in MCF7 cells treated with HDZC and were attributed to the mitochondrial intrinsic pathway based on the up-regulation of Bax and the down-regulation of Bcl-2. HDZC induced cell cycle arrest in MCF7 cells and increased the expression of p21 and p27 at the mRNA and protein levels. This results of this study substantiate the anticancer effect of TPHE and highlight the involvement of HDZC as one of the contributing compounds that act by initiating mitochondrial-mediated apoptosis. © 2015 S. Karger AG, Basel.

  18. Biosynthesis of Germacrene A Carboxylic Acid in Chicory Roots. Demonstration of a Cytochrome P450 (+)-Germacrene A Hydroxylase and NADP+-Dependent Sesquiterpenoid Dehydrogenase(s) Involved in Sesquiterpene Lactone Biosynthesis

    Science.gov (United States)

    de Kraker, Jan-Willem; Franssen, Maurice C. R.; Dalm, Marcella C. F.; de Groot, Aede; Bouwmeester, Harro J.

    2001-01-01

    Sprouts of chicory (Cichorium intybus), a vegetable grown in the dark, have a slightly bitter taste associated with the presence of guaianolides, eudesmanolides, and germacranolides. The committed step in the biosynthesis of these compounds is catalyzed by a (+)-germacrene A synthase. Formation of the lactone ring is the postulated next step in biosynthesis of the germacrene-derived sesquiterpene lactones. The present study confirms this hypothesis by isolation of enzyme activities from chicory roots that introduce a carboxylic acid function in the germacrene A isopropenyl side chain, which is necessary for lactone ring formation. (+)-Germacrene A is hydroxylated to germacra-1(10),4,11(13)-trien-12-ol by a cytochrome P450 enzyme, and is subsequently oxidized to germacra-1(10),4,11(13)-trien-12-oic acid by NADP+-dependent dehydrogenase(s). Both oxidized germacrenes were detected as their Cope-rearrangement products elema-1,3,11(13)-trien-12-ol and elema-1,3,11(13)-trien-12-oic acid, respectively. The cyclization products of germacra-1(10),4,11(13)-trien-12-ol, i.e. costol, were also observed. The (+)-germacrene A hydroxylase is inhibited by carbon monoxide (blue-light reversible), has an optimum pH at 8.0, and hydroxylates β-elemene with a modest degree of enantioselectivity. PMID:11299372

  19. Estudo comparativo entre duas técnicas radiográficas transcranianas para estudo da ATM

    OpenAIRE

    Oliveira, Marcus Vinícius Linhares de; Campos, Paulo Sérgio Flores

    2012-01-01

    p. 189-193 Introdução: Estudos através das imagens da ATM apresentam-se como auxílio ao diagnóstico especifico de determinadas patologias relacionadas a essa estrutura anatômica. De modo a oferecer mais informações possíveis, visualização mais clara, redução das dificuldades de interpretação radiográfica, é imperativa a boa execução das técnicas radiográficas e o desenvolvimento de novos métodos de exposição. Objetivo: Avaliar duas incidências radiográficas convencionais que po...

  20. Estudos de medicamentos biosimilares Studies on biosimilar medications

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    Winston Bonetti Yoshida

    2010-09-01

    Full Text Available No Brasil, o registro de novos medicamentos é feito apenas quando a agência reguladora - Agência Nacional de Vigilância Sanitária (Anvisa - se satisfaz plenamente com as evidências de sua qualidade, eficácia e segurança, apresentadas por uma indústria farmacêutica que pleiteie esse registro. Com o vencimento de patentes, empresas farmacêuticas se sentem atraídas pela produção medicamentos biológicos chamados de biosimilares ou biogenéricos ou simplesmente genéricos, cuja aprovação pode resultar em redução de custos de tratamento. Mas é preciso que o biosimilar seja, pelo menos, igualmente eficaz e seguro e sem contaminantes em relação ao original. Consensos recentes apontam diretrizes para estabelecer critérios de eficácia e segurança desses medicamentos. Estudos pré-clínicos in vitro e in vivo, procedência da matéria-prima e estudos clínicos fase I, II e III são preconizados para registro do produto biosimilar no mercado internacional. As heparinas de baixo peso molecular encontram-se nessa situação. Nesta revisão, abordamos especificamente esse tipo de medicamento, o que pode servir de parâmetro para outros biosimilares.In Brazil, the registration of new drugs is carried out only when the regulatory agency (Anvisa, acronym in Portuguese is fully satisfied with the evidence of their quality, efficacy and safety, presented by a pharmaceutical industry that strive for this registration. With the patent expiration, pharmaceutical companies are attracted to produce biological medicines called biosimilar or biogenerics or simply generics, whose approval may result in reduced treatment costs. But it is necessary that the biosimilar be, at least, equally effective and safe and without contaminants in relation to the original. Recent consensus guidelines aim to establish criteria for efficacy and safety of these medicines. Preclinical studies in vitro and in vivo, the origin of raw materials and clinical studies

  1. Imagem organizacional: um estudo de caso sobre a PUC Minas.

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    Alexandre de Pádua Carrieri

    2008-06-01

    enquanto objeto de estudo, utilizou-se como estratégia metodológica a pesquisa qualitativa, com a análise de conteúdo. O trabalho foi realizado em duas etapas: entrevistas com os gestores da Universidade e pesquisa de campo com segmentos de públicos de interesse tais como: professores e alunos de escolas de segundo grau, alunos de graduação e profissionais de recursos humanos. Observou-se que novas formas de relacionamento entre a organização e seus públicos de interesse foram criadas. Foram também implantados novos meios de comunicação – mais modernos, ágeis e editorialmente trabalhados – e estruturados novos processos de comunicação e interlocução, oportunizando melhor controle para a alta administração, da imagem da sua Universidade. Palavras-chave: Estudos organizacionais; imagem organizacional; universidade Abstract This research has purpose know a little more about corporative image, considering its importance to management of organizations in current context of higher competition and greater demand. This study came from the configuration of the culture and identity of organization, foreseeing those items as fundamentals to definition of any institution’s image. Taking the Pontifícia Universidade Católica de Minas Gerais - PUC Minas – as object of study, the content analysis was carried out through a qualitative research - the employed methodological strategy. The work was conducted in two stages: (I interviews with managers, and (II field research with public segments, such as professors and high school students, undergraduate students, and HR professionals. By way of installing innovative, modern, agile and skillfully edited communication channels, new means of relationship were created among the organization and its stakeholders. Those original processes of communication and dialogue further enhanced the control of the university’s public image by its higher administration. Keywords: Organizational studies, organizational

  2. Cuidados paliativos e luto: um estudo bibliométrico

    Directory of Open Access Journals (Sweden)

    Maria Andréa Fernandes

    Full Text Available RESUMO Analisar a produção científica, publicada em periódicos online, sobre os cuidados paliativos e luto. Trata-se de um estudo bibliométrico, realizado com 48 artigos, publicados entre 2005 e 2014, selecionados nas bases de dados LILACS, MEDLINE, IBECS, PubMed, SciELO e DOAJ. Os anos de 2012 e 2013 apresentaram um quantitativo maior de publicações. Foram identificados 37 veículos de divulgação, dos quais o periódico Medicina Paliativa Journal foi o que mais publicou sobre a temática, e o American Journal of Psychiatry apresentou o maior fator de impacto. A maioria das publicações foi produzida por pesquisadores da área de Medicina e predominaram autores com o título de doutor. Espera-se ter contribuído para estimular as produções científicas nessa temática e subsidiar na difusão desse conhecimento específico e auxiliar na preparação e formação dos profissionais para cuidado ao paciente sob cuidados paliativos no enfrentamento do luto.

  3. O trabalho e o alcoolismo: estudo com trabalhadores

    Directory of Open Access Journals (Sweden)

    Verginia Medianeira Dallago Rossato

    2004-06-01

    Full Text Available Esta investigação foi realizada com trabalhadores de uma instituição pública, tendo por objetivos analisar a influência do trabalho na produção/reprodução e transformação do comportamento alcoolista. Metodologicamente este estudo se caracteriza como exploratório e qualitativo, tendo por técnicas de coleta de dados a análise documental e a observação participante. Entrevistas foram realizadas quando se fizeram necessárias para complementar a informação. A identificação de nexos de produção e reprodução do comportamento alcoolista no contexto institucional possibilitou propostas para sua transformação, bem como mostrou a necessidade de se fazer um trabalho integrado entre os serviços que tratam do alcoolista, ao se buscar outros investimentos para mudanças gradativas deste comportamento.

  4. ESTUDO DE EVENTOS CO-SISMOGÊNICOS BRASILEIROS

    Directory of Open Access Journals (Sweden)

    Thiago de Almeida Santos

    2017-02-01

    Full Text Available Este trabalho, relacionado com o evento sísmico de 16 de Setembro de 2015, em Illapel, Chile, foca na análise de variações geomagnéticas, principalmente na caracterização e situação em que se dão, a fim de contribuir para uma melhor compreensão dos processos físicos envolvidos no acoplamento Litosfera-Atmosfera-Ionosfera (LAI. Para isto, foram utilizados registros de magnetômetros fluxgate medidos em superfície fornecido pela rede EMBRACE. A metodologia de análise consiste na decomposição do sinal por bandas de frequência, isto é, Decomposição de Modo Empírico (EMD. O resultado alcançado no momento é a caracterização de efeitos magnéticos co-sismogênicos em território brasileiro causados pelo evento sísmico citado. Portanto, estudos como este se mostram indispensáveis e importantes não apenas no ponto de vista científico, mas também pela possibilidade de contribuição para uma futura condição de previsão e de potencial de alerta de catástrofes.

  5. Conhecimento organizacional em escolas de governo: um estudo comparado

    Directory of Open Access Journals (Sweden)

    Anderson Macedo de Jesus

    2012-08-01

    Full Text Available Como reflexo de uma tendência que tem ganhado importância crescente nas organizações públicas, privadas e não governamentais do Brasil e do mundo, a temática gestão do conhecimento motivou a realização desta pesquisa, cujo foco imediato é fornecer subsídios para auxiliar organizações públicas como escolas de governo em seus processos de desenvolvimento de conhecimento organizacional. Este conhecimento é criado mediante a interação dos conhecimentos tácito e explícito. A interação entre eles pode ser verificada por meio de quatro conversões, as quais formam um ciclo do conhecimento organizacional: a socialização, a externalização, a combinação e a internalização. O estudo em questão visa apresentar um conjunto de critérios representantes do ciclo de desenvolvimento do conhecimento organizacional e, mediante análise comparada entre sete escolas de governo internacionais e uma nacional, a Fundação Escola Nacional de Administração Pública (Enap, elaborar o desenho de uma estrutura organizacional que maximize o desenvolvimento do conhecimento organizacional em escolas de governo.

  6. Negociação empresarial: um estudo multicasos

    Directory of Open Access Journals (Sweden)

    Harrison Bachion Ceribeli

    2016-03-01

    Full Text Available Este trabalho objetivou analisar como diferentes fatores influenciam os resultados de uma negociação. A metodologia empregada foi o desenvolvimento de dois estudos de caso, com o intuito de analisar dois processos de negociação entre funcionários do nível operacional e da alta gerência. Os dados foram coletados por meio de observação direta e entrevistas em profundidade. Como conclusões, pode-se verificar que a comunicação efetiva entre as partes é fundamental, e que elementos como respeito mútuo e utilização de informações não apenas numéricas são essenciais para garantir que as partes entendam as necessidades do outro. Também se constatou que os negociadores devem se mostrar abertos à negociação e que esta se torna mais efetiva à medida que os envolvidos sentem que contribuíram para o acordo. Finalmente, destaca-se a importância da confiança mútua e da ética por parte de todos, aumentando a possibilidade de se chegar a um acordo favorável aos interessados.

  7. Do Antissocialismo ao Anticapitalismo: um estudo sobre a Rerum Novarum

    Directory of Open Access Journals (Sweden)

    Claudinei Magno Magre Mendes

    2016-05-01

    Full Text Available O objetivo deste artigo é examinar o processo de constituição do anticapitalismo por meio da análise da Rerum Novarum, a primeira encíclica social. Comparando esta com as encíclicas anteriores do século XIX, pudemos estabelecer o que ela trouxe de novidade no que diz respeito ao combate que a Igreja travava contra o socialismo. Com essa comparação, foi possível caracterizar a postura anticapitalista, confrontando a nossa maneira de concebê-la com as demais. Para o exame da Rerum Novarum foi igualmente importante analisar alguns estudos referentes a ela, tanto os que apareceram logo após a sua publicação como os que foram realizados em décadas mais recentes. Como conclusão, definimos o anticapitalismo como uma atitude que somente poderia ter surgido em condições sociais e políticas bastante particulares e que apenas apareceram em fins do século XIX.

  8. Dermatotilexomania em estudantes de medicina: um estudo piloto

    Directory of Open Access Journals (Sweden)

    Ferrão Ygor Arzeno

    1999-01-01

    Full Text Available INTRODUÇÃO: Dermatotilexomania pode ser classificada, segundo o DSM-IV, dentro do capítulo de transtornos de controle de impulsos não classificados em outro lugar. Autores têm incluído o distúrbio dentro do espectro obsessivo-compulsivo. O objetivo deste trabalho é identificar a prevalência de dermatotilexomania em estudantes de medicina de uma faculdade de Porto Alegre (RS. MATERIAL E MÉTODO: Os autores realizaram um levantamento das características clínicas e demográficas dessa população. Um questionário adaptado foi aplicado a 144 estudantes de medicina. RESULTADOS: Destes, 89 (61,8% já haviam causado ou agravado lesões à pele no passado ou ainda o fazem; 12 (8,3% responderam que lesionam mais a pele do que a maioria das pessoas e 1 (0,7% classificou sua dermatotilexomania como extrema. Mais da metade dos estudantes (n=80, 55,6% consideraram o ato como um mau hábito. CONCLUSÕES: O estudo sugere que a o ato de causar ou agravar lesões à pele parece ser comum entre jovens, mas não necessariamente deve ser considerado como patológico. Para os casos mais graves, entretanto, a condição poderia ser classificada como um transtorno: dermatotilexomania.

  9. ESTUDO DA MATURIDADE DE PROCESSOS COBIT NA ELETROBRÁS

    Directory of Open Access Journals (Sweden)

    Fernando Cesar Almeida Silva

    2017-09-01

    Full Text Available Este artigo tem como objetivo descrever como foi realizado um estudo da maturidade dos processos CobiT em uma empresa do setor de energia elétrica do Brasil. Foi feita uma pesquisa onde os integrantes do Comitê de Tecnologia de Informação desta empresa informam sua percepção do nível de maturidade dos diversos processos existentes no CobiT. É realizada uma análise dos resultados onde é considerado o contexto no qual a empresa se insere. Para finalizar é proposto um sistema baseado no PDCA com o objetivo de aprimorar os processos que receberam classificação de avaliação mais baixa. A priorização de quais processos devem ser aprimorados primeiro, é baseada em critérios que levam em consideração os principais controles de ambiente do PCAOB, uma vez que esta empresa é auditada regularmente devido às obrigatoriedades da lei Sarbanes-Oxley.

  10. Trabalho e riscos de adoecimento: um estudo entre policiais civis

    Directory of Open Access Journals (Sweden)

    Vânia Cristine Cavalcante Anchieta

    Full Text Available Este estudo teve como objetivo avaliar a percepção que os policiais civis do DF têm sobre o seu contexto de trabalho, suas exigências, assim como as vivências e os problemas físicos, psicológicos e sociais causados pelo trabalho, procurando fazer inferências sobre as estratégias de mediação utilizadas para evitar o sofrimento e os riscos de adoecimento. Foi aplicado o Inventário do Trabalho e Riscos de Adoecimento (ITRA em 160 policiais civis, homens e mulheres, recém empossados na Instituição (tempo médio de oito meses de ingresso na Instituição. Os resultados indicaram que, apesar de não ficarem evidentes danos graves à saúde do policial novato, há riscos de acontecerem falhas nas estratégias de mediação em relação a fatores que levam ao adoecimento.

  11. Desordens alimentares e perfeccionismo: um estudo com atletas portugueses

    Directory of Open Access Journals (Sweden)

    Rui Gomes

    2010-12-01

    Full Text Available Este trabalho analisa a relação entre desordens alimentares e tendência para o perfeccionismo no desporto. Participaram no estudo 299 atletas a competirem nas principais divisões de diferentes modalidades. Foi aplicado um protocolo de avaliação com o Questionário de Avaliação das Desordens Alimentares (Fairburn & Beglin, 1994 e a Escala Multidimensional de Perfeccionismo no Desporto (Dunn et alii, 2006. Aplicaram-se procedimentos de análise de variância multivariada (Manovas e análises de regressão hierárquica no tratamento dos dados. Dois resultados devem ser salientados: i atletas mais novos e atletas com maior tendência para as desordens alimentares evidenciaram maior perfeccionismo; ii a dimensão de preocupação com os erros foi a principal variável preditora das desordens alimentares. Concluindo, observou-se uma relação entre a tendência para as desordens alimentares e a orientação para a exigência pessoal e preocupação com os erros por parte dos atletas

  12. Raiva humana: estudo neuropatológico de trinta casos

    Directory of Open Access Journals (Sweden)

    R. V. C. Assis

    1984-12-01

    Full Text Available O estudo epidemiológico e neuropatológico concernente a trinta autópsias de encefalite rábica foi realizado, tendo sido os dados obtidos comparados com aqueles da literatura. Não houve relação estatística entre a topografia lesional e o local da mordida, entre o período de incubação ou a duração da doença e a presença ou ausência de inclusões virais (IV assim como entre a intensidade do processo inflamatorio (PI e a presença de IV. A dispersão do PI e das IV através o SNC foi diretamente proporcional à sua intensidade. A estrutura mais freqüentemente comprometida pelo PI foi o mesencéfalo, seguido pelo bulbo, ponte e medula espinhal. A estrutura mais intensamente afetada foi o bulbo. As IV foram particularmente proeminentes no hipocampo e cerebelo.

  13. Atitudes do professor em sala de aula – um estudo

    Directory of Open Access Journals (Sweden)

    Maria Cristina Marcelino Bento

    2014-01-01

    Full Text Available A maneira como o professor se comporta, e a maneira do médico podem ser comparadas. Um médico que fica nervoso na frente de seu paciente, que treme ao lidar com agulhas ou, desmaia ao ver sangue, e o professor que não sabe lidar com alunos que choram, alunos que possuem uma cultura muito diferente dos demais e do próprio professor, estão despreparados para a sua profissão. Torna-se pois, questionável se o professor sabe da importância que seus atos têm sobre seus alunos.Têm-se como objetivos: verificar na literatura a importância da linguagem corporal para o processo de aprendizagem; verificar se docentes de uma escola pública percebem a importância dos gestos e/ou atitudes para o processo de aprendizagem dos alunos. Pesquisa de natureza exploratória, com delineamento em estudo de caso e análise qualitativa, foi realizada em uma escola pública do Vale do Paraíba, Estado de São Paulo, com docentes do Ensino Fundamental I. Conclui-se que os professores precisam estar atentos a suas atitudes e sua maneira de agir, pois uma palavra do professor pode resultar tanto em uma ferida para o resto da vida, quanto para um avanço em aprendizagem escolar e desenvolvimento do aluno.

  14. Estudo da auditoria de contas em um hospital de ensino

    Directory of Open Access Journals (Sweden)

    Gabriela Favaro Faria Guerrer

    2015-06-01

    Full Text Available RESUMOObjetivos:verificar os itens componentes das contas hospitalares, conferidos por enfermeiros auditores, que mais recebem ajustes no momento da pré-análise; identificar o impacto dos ajustes no faturamento das contas analisadas por enfermeiros e médicos auditores e identificar as glosas relacionadas aos itens conferidos pela equipe de auditoria.Método:pesquisa quantitativa exploratória, descritiva, do tipo estudo de caso único.Resultados:após a análise de 2.613 contas constatou-se que o item mais incluído por enfermeiros foram gases (90,5% e o mais excluído medicamentos de internação (41,2%. Materiais de hemodinâmica; gases e equipamentos foram os que mais impactaram nos ajustes positivos. Os ajustes negativos decorreram de lançamentos indevidos nas contas e não geraram prejuízos de faturamento. Do total de glosas 52,24% referiu-se à pré-análise dos enfermeiros e 47,76% a dos médicos.Conclusão:a presente investigação do processo de pré-análise fornece subsídios que contribuem para o avanço no conhecimento sobre a auditoria de contas hospitalares.

  15. Estudo de usuários on line

    Directory of Open Access Journals (Sweden)

    Bruno Macedo Nathansohn

    2005-07-01

    Full Text Available Trata-se de um estudo de usuários baseado na perspectiva da responsabilidade social da Ciência da Informação, considerando um agregado de informação disponível na Internet. O objetivo foi o levantamento do perfil dos usuários, para detectar suas preferências em relação ao sítio, assim como a investigação sobre o uso que se faz de um espaço que produz informação política, econômica e social. Para tanto, foram desenvolvidos mecanismos de interatividade para que, ao mesmo tempo em que houvesse a coleta de dados por parte do profissional da informação proporcionasse a participação, por parte dos usuários, para a possibilidade de transformar a estrutura desse agregado. Processo que se baseou na metodologia da Pesquisa-Ação, identificando e apresentando as barreiras na comunicação, como resultado final do processo de intervenção científica.

  16. Aprendizagem Organizacional, Teoria Ator-Rede e Estudos Foucaultianos: Tempo, Espaço e Poder

    OpenAIRE

    Bruno Luiz Américo

    2016-01-01

    Esse trabalho baseia-se no mapeamento de lacunas encontradas nos desenvolvimentos dos estudos organizacionais de conhecimento e aprendizagem, e busca contribuir para a consolidação da aprendizagem organizacional como uma teoria do movimento organizacional/social. Para isso, propõe diálogo entre a Perspectiva Social-Política da Aprendizagem social, a Teoria Ator-Rede e os estudos foucaultianos. O trabalho conclui destacando o potencial da teoria ator-rede e dos estudos foucaultianos para anali...

  17. Estudos taxonômicos e filogenéticos em Miconia sect. Discolor (Melastomataceae, Miconieae)

    OpenAIRE

    Mayara Krasinski Caddah

    2013-01-01

    Resumo: Miconia é um é um dos maiores gêneros de angiospermas. Tradicionalmente, tem sido considerado complexo e mal circumscrito, assim como outros gêneros aparentados. Estudos filogenéticos recentes, baseados em marcadores moleculares, têm atestado a natureza polifilética de Miconia e suas onze seções. Neste trabalho, é apresentado um estudo filogenético-molecular aprofundado de um dos grupos naturais encontrados em estudos anteriores, o "subclado Miconia discolor", do "clado Miconia IV". P...

  18. Auditoria interna de sistema de gestão ambiental: estudo de caso no Verdegreen Hotel

    OpenAIRE

    Adriano Lucena da Silva; Isaiane Vieira de Melo; Diego Rodrigues de Lucena

    2017-01-01

    Esta pesquisa analisa as contribuições e os desafios associados às auditorias internas no Verdegreen Hotel, empreendimento hoteleiro de grande destaque na cidade de João Pessoa-PB, selecionado como unidade de estudo por dispor de um Sistema de Gestão Ambiental (SGA) ISO 14001 implantado e certificado. Este estudo trata de uma pesquisa descritiva, baseada em um estudo de caso qualitativo. As técnicas utilizadas na metodologia foram: levantamento bibliográfico sobre as normas ISO que abordam as...

  19. Um estudo comparativo entre dados sociodemográficos e neuroticismo

    OpenAIRE

    Mansueto Dal Maso; Fabio Biasotto Feitosa

    2013-01-01

    O neuroticismo tem sido referido como um importante indicador de saúde mental. O estudo do grau de neuroticismo em uma dada população pode ser estratégico para o planejamento de políticas e programas de saúde coletiva. O objetivo do presente estudo foi avaliar o neuroticismo em estudantes universitários do Estado de Rondônia. Participaram como sujeitos deste estudo de levantamento 1.031 universitários de ambos os sexos, entre 18 e 75 anos, que autoavaliaram seu grau de neuroticismo. Os dados ...

  20. Aumento da prematuridade no Brasil: revisão de estudos de base populacional

    OpenAIRE

    Silveira,Mariângela F; Santos,Iná S; Barros,Aluísio J D; Matijasevich,Alicia; Barros,Fernando C; Victora,Cesar G

    2008-01-01

    OBJETIVO: A maior causa de mortalidade infantil no Brasil são condições perinatais, associadas em sua maioria à prematuridade. O objetivo do estudo foi avaliar a evolução das taxas de prematuridade no Brasil. MÉTODOS: Foi realizada revisão nas bases de dados Medline e Lilacs, incluindo estudos publicados em periódicos, teses e dissertações, desde 1950. Os critérios de exclusão foram: estudos que se referiam a temas clínicos, com complicações da prematuridade e gestação, bem como cuidados com ...

  1. Sesquiterpene dimer (DSF-52) from Artemisia argyi inhibits microglia-mediated neuroinflammation via suppression of NF-κB, JNK/p38 MAPKs and Jak2/Stat3 signaling pathways.

    Science.gov (United States)

    Zeng, Ke-Wu; Wang, Shu; Dong, Xin; Jiang, Yong; Tu, Peng-Fei

    2014-02-15

    Microglia-involved neuroinflammation is thought to promote brain damage in various neurodegenerative disorders. Therefore, novel therapeutics suppressing microglia over-activation could prove useful for neuroprotection in inflammation-mediated neurodegenerative diseases. DSF-52 is a novel sesquiterpene dimer compound isolated from medical plant Artemisia argyi by our group. In this study, we investigated whether DSF-52 inhibited the neuroinflammatory responses in lipopolysaccharide (LPS)-activated microglia. Our findings showed that DSF-52 inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), as well as mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), interleukin-1β (IL-1β), granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage inflammatory protein-1α (MIP-1α) in LPS-activated BV-2 microglia. Moreover, DSF-52 markedly up-regulated mRNA levels of anti-inflammatory cytokine IL-10. Mechanism study indicated that DSF-52 suppressed Akt/IκB/NF-κB inflammation pathway against LPS treatment. Also, DSF-52 down-regulated the phosphorylation levels of JNK and p38 MAPKs, but not ERK. Furthermore, DSF-52 blocked Jak2/Stat3 dependent inflammation pathway through inhibiting Jak2 and Stat3 phosphorylation, as well as Stat3 nuclear translocation. We concluded that the inhibitory ability of DSF-52 on microglia-mediated neuroinflammation may offer a novel neuroprotective modality and could be potentially useful in inflammation-mediated neurodegenerative diseases. Copyright © 2013 Elsevier GmbH. All rights reserved.

  2. Gerência de Informação em Estudos Multicêntricos: o Estudo Longitudinal de Saúde do Adulto

    OpenAIRE

    Duncan, Bruce Bartholow; Vigo, Álvaro; Hernandez, Émerson; Luft, Vivian Cristine; Ahlert, Hubert; Bergmann, Kaiser; Mota, Eduardo

    2013-01-01

    A gerência da informação em estudos multicêntricos de grande porte requer uma abordagem especializada. O Estudo Longitudinal da Saúde do Adulto (ELSA-Brasil) criou um Centro de Dados para delinear e gerenciar seu sistema de dados. O objetivo do artigo foi descrever os passos envolvidos, incluindo os métodos de entrada, transmissão e gerência de informações. Foi desenvolvido um sistema web que permitiu, de forma segura e confidencial, a entrada online, verificação e edição, bem como incorpo...

  3. Gerência de informação em estudos multicêntricos: o estudo longitudinal de saúde do adulto

    OpenAIRE

    Duncan, Bruce Bartholow; Vigo, Álvaro; Hernandez, Émerson; Luft, Vivian Cristine; Ahlert, Hubert; Bergmann, Kaiser; Mota, Eduardo

    2013-01-01

    P. 95-104 A gerência da informação em estudos multicêntricos de grande porte requer uma abordagem especializada. O Estudo Longitudinal da Saúde do Adulto (ELSA-Brasil) criou um Centro de Dados para delinear e gerenciar seu sistema de dados. O objetivo do artigo foi descrever os passos envolvidos, incluindo os métodos de entrada, transmissão e gerência de informações. Foi desenvolvido um sistema web que permitiu, de forma segura e confidencial, a entrada online, verificação e edição, bem co...

  4. Gerência de Informação em Estudos Multicêntricos: o Estudo Longitudinal de Saúde do Adulto

    OpenAIRE

    Duncan,Bruce Bartholow; Vigo,Álvaro; Hernandez,Émerson; Luft,Vivian Cristine; Ahlert,Hubert; Bergmann,Kaiser; Mota,Eduardo

    2013-01-01

    A gerência da informação em estudos multicêntricos de grande porte requer uma abordagem especializada. O Estudo Longitudinal da Saúde do Adulto (ELSA-Brasil) criou um Centro de Dados para delinear e gerenciar seu sistema de dados. O objetivo do artigo foi descrever os passos envolvidos, incluindo os métodos de entrada, transmissão e gerência de informações. Foi desenvolvido um sistema web que permitiu, de forma segura e confidencial, a entrada online, verificação e edição, bem como incorporaç...

  5. Lesões esportivas: um estudo com atletas do basquetebol bauruense

    Directory of Open Access Journals (Sweden)

    Bruno Estevan Siqueira Dario

    2010-05-01

    Full Text Available Este estudo teve como objetivo identificar as principais lesões no basquetebol e suas incidências. Participaram do estudo vinte jogadores de basquetebol do sexo masculino com idade variando entre 13 e 15 anos. O instrumento utilizado foi o questionário e seus resultados mostraram que, dos atletas participantes do estudo, todos relataram algum tipo de lesão. Foram identificadas 26 lesões, sendo 13 nos membros inferiores (50% e 13 nos membros superiores (50%. No joelho, as lesões mais frequentes foram a tendinite patelar - sete casos (53,85%. Nos membros superiores, destacaram-se as entorses nos dedos das mãos - cinco casos (38,46%. Com este estudo, percebemos a necessidade de se desenvolver um trabalho preventivo no intuito de diminuir a incidência dessas lesões.

  6. ESTUDOS MEDIEVALISTAS DO NORTE EUROPEU E O ESTADO DA ARTE NO BRASIL

    Directory of Open Access Journals (Sweden)

    Amanda Basilio Santos

    2018-02-01

    Full Text Available Este artigo pretende abordar o estado da questão dos estudos medievais que se dedicam à análise histórica do norte europeu no período medieval, assim como discutir o aprofundamento do entendimento destas regiões através da ampliação das fontes utilizadas pelos historiadores. Faremos um breve apanhado do desenvolvimento da historiografia europeia neste sentido, assim como discutiremos a ampliação do interesse dos pesquisadores brasileiros por esta área em questão nos últimos anos, destacando a criação de núcleos de pesquisas direcionados ao estudo do norte da Europa na Idade Média, em especial o NEVE (Núcleo de Estudos Vikings e Escandinavos e o NEIBRAM (Núcleo de Estudos Interdisciplinares das Ilhas Britânicas: Antiguidade e Medievo.

  7. COMPORTAMENTO DO CONSUMIDOR ENDIVIDADO: UM ESTUDO EXPLORATÓRIO COM INDIVÍDUOS DE BAIXA RENDA

    Directory of Open Access Journals (Sweden)

    Celso Augusto de Matos

    2015-02-01

    Full Text Available O presente estudo teve como objetivo geral analisar os fatores associados ao superendividamento de consumidores de baixa renda. Foi realizado um estudo exploratório, de cunho qualitativo, por meio de entrevistas individuais com consumidores das classes D e E. Dentre os principais fatores associados ao acúmulo de dívidas pelos entrevistados, destacam-se as compras por impulso, o pagamento do valor mínimo das faturas do cartão de crédito, o uso de financiamentos e empréstimos para aquisição de bens ou até mesmo para pagar outra dívida e fatores imprevistos. O estudo termina com uma discussão teórica destes resultados e sugestões de pesquisa para estudos futuros.

  8. Atitude religiosa e sentido da vida: um estudo correlacional

    Directory of Open Access Journals (Sweden)

    Thiago Antônio Avellar de Aquino

    Full Text Available A presente pesquisa abordou o sentido da vida (Viktor Frankl e a atitude religiosa. Para a logoterapia, a religiosidade não significa necessariamente a opção por uma crença religiosa, mas pode ser uma das possíveis maneiras de o homem encontrar sentido para a vida. O objetivo deste estudo foi investigar a relação entre o sentido de vida e a atitude religiosa de forma transversal e correlacional. A amostra foi composta por 300 sujeitos de ambos os sexos, 37% homens e 63% mulheres, com idade média de 42 anos. Os instrumentos utilizados foram o Teste Propósito de Vida (PIL-Test, de James C. Crumbauch e Leonard T. Maholick, e a Escala de Atitude Religiosa/Espiritualidade, de Aquino. Os resultados apontam correlações positivas entre a atitude religiosa e a realização existencial, a atitude religiosa e a idade, o desespero existencial e o vazio existencial e a realização existencial e a idade, havendo também correlações negativas entre a atitude religiosa e o desespero existencial, a atitude religiosa e o vazio existencial, o desespero existencial e a realização existencial e o vazio existencial e a realização existencial. Concluiu-se que a atitude religiosa é uma forma de encontro de sentido de vida bem como um elemento de prevenção do vazio existencial e do desespero existencial.

  9. Apoiando estudos cientométricos com Linked Open Data

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    Sandro Rautenberg

    Full Text Available RESUMO Na Cientometria, mensurar indicadores é uma tarefa complexa devido aos desafios em coletar, organizar e relacionar dados, sobretudo, na web, onde os dados são distribuídos em várias fontes e formatos incompatíveis. Esses problemas podem ser resolvidos com o emprego de tecnologias e metodologias baseadas nos princípios Linked Open Data. Tais princípios são fundamentados num conjunto de melhores práticas de Web Semântica e Dados Abertos para organizar, publicar e conectar dados na web. Por meio destes, os dados são acessados e consumidos sem restrições, em diversas aplicações. No presente trabalho, relata-se a experiência na disponibilização do histórico do índice Qualis conforme o Linked Open Data. Pressupõe-se que tal empreendimento é importante nas atividades de coleta de dados primários em pesquisas bibliométricas/cientométricas, servindo para: mensurar a evolução dos periódicos científicos; auxiliar na aferição de medidas qualitativas e quantitativas de publicações científicas; ou obter informações relevantes a partir do cruzamento com outros indicadores cientométricos. A disponibilização do índice Qualis é verificada em três estudos de caso. Como resultado, têm-se o compartilhamento do índice Qualis (série histórica 2005-2013 por uma interface web para: (i facilitar o reuso e a integração dos dados; e (ii suportar a interoperabilidade e a processabilidade computacional dos recursos disponibilizados.

  10. ACREDITAÇÃO HOSPITALAR: ESTUDO DE CASOS NO BRASIL

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    Luci Emidio

    2013-06-01

    Full Text Available A contínua introdução de novas tecnologias no ambiente hospitalar exige processos educacionais permanentes tanto na área técnica quanto gerencial. A qualidade dos serviços de saúde e seu contínuo aperfeiçoamento são de interesse público no qual representam um indicador do atendimento de demandas sociais e da eficiência do Estado. No setor empresarial representam um diferencial na escolha de hospitais. O objetivo da pesquisa foi descrever o processo de implantação da gestão da qualidade dos processos assistenciais, envolvendo a gestão clínica e a gestão administrativa de modo particular, para constituir um mapeamento das ações rotineiras para manter o selo de hospital acreditado. O método foi o estudo de casos múltiplos e a unidade de análise a acreditação hospitalar. Participaram cinco hospitais acreditados pela Organização Nacional de Acreditação e pelo Consórcio Brasileiro de Acreditação, são provenientes das cidades de Brasília, São Paulo e Porto Alegre. Os resultados demonstram as melhorias obtidas, os fatores facilitadores e dificultadores do processo de acreditação nas instituições hospitalares. As evidencias investigadas levam a concluir, dentre outros, que os processos de relacionamento da instituição com o paciente; pode garantir maior segurança ao paciente e ao público interno dos hospitais, o mapeamento e aperfeiçoamento dos processos de assistência tendo por base a inserção da acreditação como incremento qualitativo dos hospitais.

  11. Toxoplasmose em primatas neotropicais: estudo retrospectivo de sete casos

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    Renata A. Casagrande

    2013-01-01

    Full Text Available A toxoplasmose é considerada uma doença parasitária fatal em primatas neotropicais. O objetivo deste trabalho foi descrever, através de um estudo retrospectivo, os casos de toxoplasmose em primatas neotropicais. No período de 1999-2009 foram realizados 86 exames anatomopatológicos em primatas e a toxoplasmose foi a enfermidade mais comum (7/86, relatando-se um caso em sagui-do-tufo-preto (Callithrix penicillata e seis em bugio-ruivo (Alouatta guariba. Dois animais foram encontrados mortos e cinco morreram em poucos dias. Os sinais clínicos mais frequentes foram apatia e anorexia (5/7, distensão abdominal (4/7 e febre (3/7. Na necropsia observou-se esplenomegalia (4/7, hemorragia do trato digestório, linfonodos e bexiga (4/7, pulmões avermelhados (3/7 e hepatomegalia (2/7. No exame histopatológico evidenciou-se hepatite (7/7, esplenite (3/7, miocardite (2/7, enterite (2/7, linfadenite (1/7 e sialite (1/7 necróticas e, pneumonia intersticial (4/7. Em fígado, pulmões, baço, coração, linfonodos e glândula salivar havia taquizoítos de Toxoplasma gondii que foram também detectados pelo exame de imuno-histoquímica anti-T. gondii em fígado, baço e pulmões (5/7. A toxoplasmose pode causar alta mortalidade em colônias de primatas neotropicais e representar mais uma ameaça à conversação dessas espécies em cativeiro. Sendo assim, medidas preventivas devem ser tomadas para evitar a contaminação desses animais.

  12. Agostinho e a "descoberta" da vontade: primeiro estudo: continuação e fim

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    Pich, Roberto Hofmeister

    2005-01-01

    Full Text Available Este é um estudo sobre o conceito de vontade na história da filosofia. O centro de interesse está na obra De libero arbitrio, de Agostinho. Tanto se procura descrever a suposta "descoberta" da vontade por Agostinho quanto analisar a coerência do conceito obtido. Trata-se do primeiro de dois estudos sobre a vontade e a liberdade em De libero arbitrio I

  13. Abcesso Pulmonar – Estudo Retrospectivo

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    M. José Augusto

    1995-11-01

    Full Text Available RESUMO: O abcesso pulmonar é definido como uma supuração colectada numa cavidade neoformada no pulmão, por uma inflamação aguda não tuberculosa. Por esta definição, estão excluídas as supuraçãoes desenvolvidas em cavidades pré existentes (quistos aereos, carcinoma escavado, bronquiectasias e as resultantes de processos bacilares (1,2,4.Os autores apresentam os resultados de uma revisão clínica de 24 casos correspondentes aos doentes internados com aquele diagnóstico desde 1 de Janeiro de 1990 a 31 de Dezembro de 1994 no Serviço de Medicina Interna do Hospital Distrital de Aveiro.O estudo incidiu na análise dos processos clínicos e radiológicos avaliando a distribuição por sexo, faixa etária, factores predisponentes, tempo de internamento, expressão clínica, radiológica, bacteriológica e finalizando com a terapêutica e evolução. SUMMARY: Lung abcess is defined as a collected suppuration in a neoformed cavity in the lung, caused by a non tuberculous inflammation. From this definition, supurations developed in pre-existant cavities (aerial cysts, escavated carcinoma, bronchiectasis and those resulting from bacillary process, are excluded.The authors submit the results of a clinical revision of 24 cases corresponding to resident patients with that diagnosis from 1st. January 1990 to 31st December 1994 in the Internal Medicine at R.D. Aveiro.The study rests on the analysis of the radiologic and clinical processes taking into account distribution by age and sex, predisposing factors, lenght of internment, radiological and clinical expression, bacteriological profile, therapy and evolution. Palavras-chave: Abcesso Pulmonar, terapêutica, radiologia, Key-Words: Lung abcess, therapy, radiology

  14. Estudo da Secagem de Frutos Tropicais do Nordeste

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    Lucas de Souza Barbosa

    2014-06-01

    Full Text Available A fruticultura é um dos segmentos mais importantes na agricultura nacional, porém um grande problema enfrentado pelos fruticultores é a conservação dos frutos maduros. As frutas tropicais caracterizam-se pelo fato de ser sazonais, com produção restrita a determinadas épocas do ano, podendo se verificar o aparente fenômeno da super produção, isto é, a quantidade oferecida na safra é maior do que a quantidade absorvida pelo mercado consumidor. A falta de tecnologias adequadas para o melhor aproveitamento dos frutos tem contribuído para um alto índice de desperdício cerca de centenas de toneladas ano. A desidratação de frutas vem sendo objeto de muitas pesquisas com o propósito de se obter técnicas que proporcionem, além de baixo custo, a manutenção da qualidade dos produtos desidratados. Neste sentido o presente trabalho objetivou o estudo da secagem convencional dos frutos tropicais (banana, abacaxi, manga e caju visando à obtenção dos frutos desidratados e a sua caracterização físico-química. Após a obtenção das amostras essas foram submetidas à secagem convencional e posteriormente realizado às analises de pH, sólidos solúveis, acidez total titulável, vitamina C e açúcares totais no Laboratório de Tecnologia de Alimentos da UFERSA e UFCG. De acordo com os resultados obtidos, os frutos desidratados apresentaram teores de nutrientes próximos aos relatados na literatura demonstrando que a secagem é uma excelente alternativa para a sua conservação, contribuindo assim para redução das perdas pós-colheita e com a agregação de valor ao produto seco.

  15. Estudos sôbre índice de siclemia

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    Ernani Martins da Silva

    1945-04-01

    Full Text Available O índice de sinclêmicos encontrado foi de 10%, examinando-se uma amostra heterógenea de 1130 indivíduos de grupo étnico negróide e mestiços desta etnia. Foi negativa a pesquisa em 120 indivíduos caucasóides e 30 mongolóides. O "índice familiar de siclêmicos" - a percentagem desses indivíduos em um grupo de famílias - fornece um resultado mais proximo do verdadeiro número de siclêmicos em uma amostra de população. O método clássico fornece um número inferior ao existente na amostra estudada. A aplicação do teste de siclemia em Jurisprudência, Antropologia e Etnografia, poderá contribuir, na qualidade de método auxiliar, no estudo de vários problemas. São indicados como métodos de escolha: o de Emmel e o de maceração de tecidos conservados em solução de formol ou alcool. Êste último permite estudos retrospectivos. A estase circulatória parece não ser fator suficiente para a passagem da condição de siclêmico para anemico. O parasitismo da hemátia pelo Plasmodium vivax não impede o fenômeno da siclisação. Infecção por plasmodídeos (P. falciparum e P. vivax não é fator suficiente para desencadear a doença. A importância da descoberta de Emmel (descoberta do fenômeno da siclisação reside no conhecimento em doença heredofamiliar de fato novo de máxima significação: a possibilidade de identificar os indivíduos ainda na fase de latência, ou seja, no período que medeia entre o nascimento e a instalação dos primeiros sintomas da doença. A relação entre casos de anemia falciforme ocorridos em gerações por vêzes muito afastadas (fenômeno denominado "skipping" - salto - pelos autores de lingua inglêsa é mantida através dos siclêmicos. Êsses indivíduos podem ser comparados aos portadores de germe no sentido empregado na profilaxia das doenças infectuosas. Exames periódicos dos siclêmicos poderiam contribuir para esclarecimento da patogenia e forneceriam dados úteis para a

  16. Contribuição ao estudo da Tripanosomiase Americana

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    Bichat de Almeida Rodrigues

    1942-01-01

    Full Text Available 1 Em trabalho sistematizado, foi estudada pela primeira vez a epidemiologia da doença de Chagas, em um trecho da região do estuário do Amazonas, cujas condições ecológicas diferem das encontradas em outras zonas onde teem sido feitas pesquisas semelhantes. 2 No local escolhido para estudo – Aura – uma localidade distante cerca de 10 km. de Belém, (Pará, não foi encontrada nenhuma infecção humana pelo S. cruzi, quer pelo exame de sangue, quer pelo xenodiagnóstico, ambos feitos em todos os habitantes (117 indivíduos. Não foi encontrada também sintomatologia atribuível à doença. 3 Em 6 xenodiagnósticos feitos em animais domésticos (5 cães e 1 gato, foi encontrado um cão infectado. Este animal pertencia á casa onde foi verificada maior infestação por Triatomídeos, com índice de infecção elevado. Pelas razões expostas no texto, porem, foi sugerida a hipótese do mesmo se ter infectado pela ingestão de vísceras de animais silvestres contaminados. 4 De 115 animais silvestres, cujo sangue foi examinado em gota espessa, 9 mostraram-se parasitados por Schizotrypanum (7,8%. Em 47 verificações pelo xenodiagnóstico, 15 animais silvestres (11 mucuras, 3 tamanduás e 1 tatú se apresentaram positivos (32,6% . O tamanduá (T. tetradactylus foi pela primeira vez assinalado como depositário natural do Schizotrypanum. 5 Como resultado de buscas feitas durante 11 meses, sendo cada domicílio inspecionado pelo menos de 15 em 15 dias, foram encontrados Triatomídeos em 7 das 36 casas existentes no povoado. Todos os 39 exemplares capturados eram adultos; a procura exaustiva não revelou a existência de formas jovens no interior das habitações. Na ordem de freqüência, as espécies encontradas foram as seguintes: P. geniculatus (29 exemplares, dos quais 7 infectados; R. pictipes ( 9 exemplares, dos quais 2 infectados; E. mucronatus ( 1 exemplar, não infectado . A grande maioria dos insetos foi capturada na segunda metade do

  17. Binding and molecular dynamic studies of sesquiterpenes (2R-acetoxymethyl-1,3,3-trimethyl-4t-(3-methyl-2-buten-1-yl)-1t-cyclohexanol) derived from marine Streptomyces sp. VITJS8 as potential anticancer agent.

    Science.gov (United States)

    Naine, S Jemimah; Devi, C Subathra; Mohanasrinivasan, V; Doss, C George Priya; Kumar, D Thirumal

    2016-03-01

    ) analysis showed that protein-ligand complex reaches equilibration state around 10 ns that illustrates the docked complex is stable. We propose the possible mechanism of sesquiterpenes to play a significant role in antitumor cascade. Hence, our studies open up a new facet for a potent drug as an anticancer agent.

  18. Ergolide, sesquiterpene lactone from Inula britannica, inhibits inducible nitric oxide synthase and cyclo-oxygenase-2 expression in RAW 264.7 macrophages through the inactivation of NF-κB

    Science.gov (United States)

    Whan Han, Jeung; Gon Lee, Byeong; Kee Kim, Yong; Woo Yoon, Jong; Kyoung Jin, Hye; Hong, Sungyoul; Young Lee, Hoi; Ro Lee, Kang; Woo Lee, Hyang

    2001-01-01

    We investigated the mechanism of suppression of inducible nitric oxide synthase (iNOS) and cyclo-oxygenase-2 (COX-2) by ergolide, sesquiterpene lactone from Inula britannica.iNOS activity in cell-free extract of LPS/IFN-γ-stimulated RAW 264.7 macrophages was markedly attenuated by the treatment with ergolide. Its inhibitory effect on iNOS was paralleled by decrease in nitrite accumulation in culture medium of LPS/IFN-γ-stimulated RAW 264.7 macrophages in a concentration-dependent manner. However, its inhibitory effect does not result from direct inhibition of the catalytic activity of NOS.Ergolide markedly decreased the production of prostaglandin E2 (PGE2) in cell-free extract of LPS/IFN-γ-stimulated RAW 264.7 macrophages in a concentration-dependent manner, without alteration of the catalytic activity of COX-2 itself.Ergolide decreased the level of iNOS and COX-2 protein, and iNOS mRNA caused by stimulation of LPS/IFN-γ in a concentration-dependent manner, as measured by Western blot and Northern blot analysis, respectively.Ergolide inhibited nuclear factor-κB (NF-κB) activation, a transcription factor necessary for iNOS and COX-2 expression in response to LPS/IFN-γ. This effect was accompanied by the parallel reduction of nuclear translocation of subunit p65 of NF-κB as well as IκB-α degradation. In addition, these effects were completely blocked by treatment of cysteine, indicating that this inhibitory effect of ergolide could be mediated by alkylation of NF-κB itself or an upstream molecule of NF-κB.Ergolide also directly inhibited the DNA-binding activity of active NF-κB in LPS/IFN-γ-pretreated RAW 264.7 macrophages.These results demonstrate that the suppression of NF-κB activation by ergolide might be attributed to the inhibition of nuclear translocation of NF-κB resulted from blockade of the degradation of IκB and the direct modification of active NF-κB, leading to the suppression of the expression of iNOS and COX-2, which play important

  19. Ergolide, sesquiterpene lactone from Inula britannica, inhibits inducible nitric oxide synthase and cyclo-oxygenase-2 expression in RAW 264.7 macrophages through the inactivation of NF-kappaB.

    Science.gov (United States)

    Whan Han, J; Gon Lee, B; Kee Kim, Y; Woo Yoon, J; Kyoung Jin, H; Hong, S; Young Lee, H; Ro Lee, K; Woo Lee, H

    2001-06-01

    We investigated the mechanism of suppression of inducible nitric oxide synthase (iNOS) and cyclo-oxygenase-2 (COX-2) by ergolide, sesquiterpene lactone from Inula britannica. iNOS activity in cell-free extract of LPS/IFN-gamma-stimulated RAW 264.7 macrophages was markedly attenuated by the treatment with ergolide. Its inhibitory effect on iNOS was paralleled by decrease in nitrite accumulation in culture medium of LPS/IFN-gamma-stimulated RAW 264.7 macrophages in a concentration-dependent manner. However, its inhibitory effect does not result from direct inhibition of the catalytic activity of NOS. Ergolide markedly decreased the production of prostaglandin E(2) (PGE(2)) in cell-free extract of LPS/IFN-gamma-stimulated RAW 264.7 macrophages in a concentration-dependent manner, without alteration of the catalytic activity of COX-2 itself. Ergolide decreased the level of iNOS and COX-2 protein, and iNOS mRNA caused by stimulation of LPS/IFN-gamma in a concentration-dependent manner, as measured by Western blot and Northern blot analysis, respectively. Ergolide inhibited nuclear factor-kappaB (NF-kappaB) activation, a transcription factor necessary for iNOS and COX-2 expression in response to LPS/IFN-gamma. This effect was accompanied by the parallel reduction of nuclear translocation of subunit p65 of NF-kappaB as well as IkappaB-alpha degradation. In addition, these effects were completely blocked by treatment of cysteine, indicating that this inhibitory effect of ergolide could be mediated by alkylation of NF-kappaB itself or an upstream molecule of NF-kappaB. Ergolide also directly inhibited the DNA-binding activity of active NF-kappaB in LPS/IFN-gamma-pretreated RAW 264.7 macrophages. These results demonstrate that the suppression of NF-kappaB activation by ergolide might be attributed to the inhibition of nuclear translocation of NF-kappaB resulted from blockade of the degradation of IkappaB and the direct modification of active NF-kappaB, leading to the

  20. Aspectos eticos em estudos longitudinais: o caso do ELSA-Brasil

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    Estela M L Aquino

    2013-06-01

    Full Text Available Historicamente a discussão acerca da eticidade dos atos em pesquisas com seres humanos privilegiou os estudos experimentais, pelo maior potencial de danos aos sujeitos envolvidos. Todavia, os estudos observacionais também envolvem riscos e suscitam questões relevantes. Neste artigo pretende-se apresentar e discutir aspectos éticos do desenvolvimento do ELSA-Brasil, um estudo longitudinal e multicêntrico, com financiamento público, no qual os sujeitos da pesquisa e pesquisadores pertencem às mesmas instituições. São descritos os procedimentos adotados para atender às exigências e compromissos éticos e a casuística que orientou as ações segundo seus princípios norteadores (beneficência, autonomia e justiça social. São apresentados alguns problemas morais que exigiram ponderação sobre riscos e benefícios na confluência com os objetivos do estudo e comentam-se peculiaridades de um estudo longitudinal e seus potenciais benefícios.

  1. O alcoolismo em Pernambuco: Estudo estatístico

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    Walderedo Ismael de Oliveira

    1945-03-01

    Full Text Available O autor procedeu ao levantamento estatístico de tôdas as admissões no Hospital de Alienados de Recife (Estado de Pernambuco, no período que vai de 1916 a 1930, chegando à conclusão que a percentagem de alcoolistas, em relação ao total das entradas, é de 12,4%. As percentagens mais baixas correspondem aos anos de 1925 (6,91% e 1920 (8,15%. O maior contingente de alcoolistas entrou nos anos de 1922 (22,67% e 1923 (17,72%. Em seguida fêz um estudo comparativo entre os dados verificados no Estado de Pernambuco e a incidência de psicoses alcoólicas em outros países. As estatísticas do período 1931-1935 permitiram chegar a conclusões mais minuciosas e elucidativas. No que diz respeito ao sexo, o autor assinala uma elevada proporção de alcoolistas do sexo feminino, em contraste com o habitualmente observado nos outros países. A incidência de alcoolistas, em relação à idade, é mais elevada entre os 31-35 anos para os homens e 26-30 anos para as mulheres. A maioria dos alcoolistas é recrutada entre os elementos nacionais. A maior contribuição de enfermos, no que toca à raça, é fornecida pelo elemento negro. É a comprovação de fenômeno já assinalado também nos Estados Unidos, onde é grande o predomínio dos alcoolistas de raça negra. Entre os casados há mais alcoolistas que entre solteiros e viúvos. A grande maioria dos enfermos provém dos homens de profissões humildes: operários, agricultores, comerciários, etc. A capital contribui com 46,6% do total das internações, fato curioso e digno de nota, porque tal percentagem provém de uma população 6 vezes menor que a do resto do Estado. O autor aventa algumas hipóteses explicativas de tal diferença, salietando as facilidades de hospitalização no centro urbano, as condições do homem da cidade e a situação alimentar do operário no Recife. É carregada a ascendência psicopática dos alcoolistas. No que diz respeito à maneira de como é usado o t

  2. Estudo de caso: neuropatia auditiva Auditory neuropathy: a study case

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    Viviane M. Parra

    2003-03-01

    Full Text Available Atualmente, podemos complementar os resultados encontrados na audiometria convencional com os obtidos nos exames eletrofisiológicos, o que nos permite diagnosticar não apenas uma perda auditiva periférica, mas também diferenciar uma perda auditiva coclear da neural, ou central. Isto nos conduziu a um grupo novo de pacientes que apresentava alteração na sincronia neural com função normal das células ciliadas externas. Esta patologia é conhecida como Neuropatia Auditiva. O objetivo deste estudo é descrever as características audiológicas de um paciente com Neuropatia Auditiva atendido no Centro de Docência e Pesquisa em Fonoaudiologia da Faculdade de Medicina da Universidade de São Paulo (FMUSP, correlacionando os achados com os encontrados na literatura. O caso descrito é de um indivíduo de 24 anos de idade, com o diagnóstico de Neuropatia Auditiva, atendido no ano de 2001 no Centro de Docência e Pesquisa em Fonoaudiologia da FMUSP. Foram realizados anamnese, audiometria tonal e vocal, medidas de imitância acústica, Emissões Otoacústicas (EOAs e Audiometria de Tronco Encefálico (ABR. Observamos no caso estudado a incompatibilidade de resultados entre audiometria tonal e testes de inteligibilidade de fala, com os exames objetivos, EOAs e ABR, onde observou-se perda auditiva com importante alteração nos testes de inteligibilidade de fala, EOAs presentes e ABR anormal. Estes dados sugerem função coclear normal e alteração da sincronia neural.Nowadays, in our clinic, we can complement the conventional audiometry results with electrophysiologic exams. Not only does allow us to diagnose a peripheral hearing loss but also to differentiate cochlear, neural or central hearing loss. It led us to a new group of patients that presented changes in neural synchrony, with normal functions of the outer ear hair cells. This pathology is known as Auditory Neuropathy. Our study purpose is describe the audiologist characteristic of a

  3. Uso do conceito de aprendizagem em estudos relacionados ao trabalho e organizações

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    Francisco Antonio Coelho Junior

    2008-01-01

    Full Text Available A literatura em comportamento organizacional apresenta diversos estudos referenciando o conceito aprendizagem aplicado no âmbito das organizações. Porém, verifica-se o uso pouco crítico ou sistemático deste conceito. O objetivo do presente artigo consiste em realizar uma análise conceitual do termo aprendizagem, justificando sua transposição para estudos organizacionais. Aplicaram-se técnicas de análise conceitual propostas por Ryle. Como resultado, verificou-se que a natureza multifacetada do conceito de aprendizagem favorece o advento das diversas definições associadas a ele. Espera-se que haja maior sistematicidade acerca do uso do termo aprendizagem aplicado em estudos organizacionais, considerando seus níveis de análise e as dimensões presentemente discutidas.

  4. Bem-estar no trabalho: estudo de revisão

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    Gustavo Barreto Santos

    2013-06-01

    Full Text Available O objetivo do presente estudo é apresentar uma revisão de literatura científica sobre o bem-estar no trabalho (BET e mostrar a produção científica na área de modo a sistematizar a condução de novos estudos. Realizou-se, entre os meses de junho e setembro de 2012, uma busca de publicações sobre o tema utilizando-se as bases Biblioteca Virtual em Saúde (Bireme, Scientific Eletronic Library Online (Scielo, PubMed e Science Direct, nas quais foram recuperados dezoito artigos para análise. Os resultados mostram a recente preocupação em definir/conceituar nos estudos brasileiros a diversidade de instrumentos e medidas utilizados e os fatores positivos e negativos que interferem no BET e, consequentemente, na saúde do trabalhador.

  5. Caso de estudo: a entrada do azeite Gallo no mercado chinês

    OpenAIRE

    Costa, Miguel Maria Portugal Martins

    2014-01-01

    Mestrado em Gestão 7 Classificação JEL: M16; F23 A elaboração desta dissertação consiste na criação de um caso de estudo pedagógico com o objectivo de tirar ilações sobre a entrada da marca “Azeite Gallo” no mercado Chinês. Optamos por desenvolver este caso de estudo através de um estudo qualitativo, suportado por um referencial teórico. O intuito do projecto é de documentar um exemplo paradigmático de um produto consumível de marca portuguesa que sucedeu em ultrapassar as barreiras ...

  6. Dança circular e qualidade de vida em mulheres mastectomizadas: um estudo piloto

    OpenAIRE

    Frison,Fernanda Sucasas; Shimo,Antonieta Keiko Kakuda; Gabriel,Mairany

    2014-01-01

    Avaliou-se a qualidade de vida de mulheres mastectomizadas que participaram do grupo de estudo e do grupo de controle antes e após a prática da dança circular. Trata-se de estudo de intervenção com duração de três meses e amostragem de 35 mulheres. Aplicaram-se os questionários World Health Organization Quality of Life-bref para avaliar a qualidade de vida e outros para caracterização da amostra. Após a intervenção, análises estatísticas mostraram diferenças significativas no grupo de estudo ...

  7. Léxico e música popular: um estudo que dá samba

    Directory of Open Access Journals (Sweden)

    Flávio de Aguiar Barbosa

    2011-06-01

    Full Text Available Como contribuição para os estudos sobre as variedades populares do português do Brasil, compilei neste estudo um corpus de sambas de três autores atuantes nas primeiras décadas do século XX, na cidade do Rio de Janeiro: Ismael Silva, Cartola e Paulo da Portela. Esses são compositores considerados modelares para o samba carioca do período, constituindo lideranças de três escolas de samba históricas da cidade: Deixa Falar, Mangueira e Portela. Neste artigo, além de apresentar brevemente o percurso da pesquisa e os resultados obtidos na análise do corpus, enfatizarei o estudo das unidades lexicais complexas e do vocabulário de especialidade do samba.

  8. Margem crítica e legitimação nos estudos para a paz

    Directory of Open Access Journals (Sweden)

    José Manuel Pureza

    2012-10-01

    Full Text Available Os Estudos para a Paz, constituídos historicamente como conhecimento crítico e, portanto, alternativo à ciência normal em Relações Internacionais, vieram, nos anos noventa, a ser cooptados pelas estruturas de regulação do sistema internacional como fundamento de muitas das opções postas em prática sobretudo no quadro dos processos de reconstrução pós-bélica. Neste contexto, o resgate da linhagem crítica dos Estudos para a Paz implica hoje duas opções radicais. A primeira é a da qualificação da paz pretendida como paz sustentável. A segunda é a da descolonização epistemológica dos Estudos para a Paz.

  9. Aprendizagem Organizacional, Teoria Ator-Rede e Estudos Foucaultianos: Tempo, Espaço e Poder

    Directory of Open Access Journals (Sweden)

    Bruno Luiz Américo

    2016-12-01

    Full Text Available Esse trabalho baseia-se no mapeamento de lacunas encontradas nos desenvolvimentos dos estudos organizacionais de conhecimento e aprendizagem, e busca contribuir para a consolidação da aprendizagem organizacional como uma teoria do movimento organizacional/social. Para isso, propõe diálogo entre a Perspectiva Social-Política da Aprendizagem social, a Teoria Ator-Rede e os estudos foucaultianos. O trabalho conclui destacando o potencial da teoria ator-rede e dos estudos foucaultianos para analisar os processos de aprendizagem organizacional, considerando-a de uma forma mais ampla e relacional, envolta em uma realidade organizacional onde há dinâmica e movimento implícito.

  10. Reflexões sobre fatos e fe(itiches no estudo das religiões

    Directory of Open Access Journals (Sweden)

    Ricardo Vieiralves-Castro

    2008-06-01

    Full Text Available Trata-se de um estudo teórico sobre o pensamento do cientista humano B. Latour e sua crítica à visão moderna sobre as religiões. Procuramos aqui discutir o conceito de simetria relacionado ao estudo das experiências religiosas, abordando especificamente a doutrina do Santo Daime, religião brasileira que utiliza um chá psicoativo em seus rituais. Relacionamos Latour às pesquisas sobre estados alterados de consciência do psiquiatra S. Grof. A busca de um olhar simétrico no estudo desta religião tem como objetivo a busca de novas maneiras de realizar pesquisas no âmbito da Psicologia Social, onde desenvolvemos uma dissertação sobre aspectos psicossociais da doutrina.

  11. Prêmio de liquidez de ações : um estudo para o mercado brasileiro

    OpenAIRE

    Chiara, Marco

    2014-01-01

    Existe uma divergência nos estudos de mercados emergentes sobre a relação entre retorno e liquidez. Enquanto nos mercados desenvolvidos é consenso que o retorno cresce com a falta de liquidez, em mercados como o Brasil concorda-se com a sua influência, mas não é unânime o crescimento da rentabilidade diante da iliquidez. Assim, este estudo busca definir as variáveis que representam o conceito de liquidez e se elas possuem influência no retorno das ações brasileiras, além de entender se existe...

  12. Homoconjugalidade masculina, revelação e redes sociais: um estudo de caso

    OpenAIRE

    Defendi, Edson Luiz

    2010-01-01

    Este estudo teve como objetivo compreender como o processo de revelação da conjugalidade homossexual masculina reflete na construção da rede social do casal e na dinâmica de seus relacionamentos. Para a construção deste estudo realizamos uma pesquisa qualitativa e interpretativa enfatizando a natureza socialmente construída da realidade e a importância de compreender o fenômeno de forma contextualizada e sistêmica. A estratégia metodológica adotada para este trabalho foi o estu...

  13. Revista Estudos Feministas Is Ten Years Old - A Brief History of Feminism in Brazil

    Directory of Open Access Journals (Sweden)

    Mirian Pillar Grossi

    2004-01-01

    Full Text Available This article is a report on the activities of Revista Estudos Feministas during its first phase at UFSC (1999-2002. It is divided into three parts: the first presents a brief review of literature about feminism and gender studies in Brazil; the second covers the move of Estudos Feministas from Rio de Janeiro to Santa Catarina, and the third presents some considerations on the role of this periodical in relation to the movement and to the field of gender, feminist and women’s studies in Brazil.

  14. Estudo bibliométrico do descritor “Ergonomia” na base LISA

    OpenAIRE

    Eliana Maria dos Santos Bahia; Raimundo Nonato dos Santos Macedo; Ursula Blattmann

    2011-01-01

    Objetivo: O estudo analisa a produção científica sobre ergonomia utilizando a base de dados Library and Information Science Abstracts (LISA). A base de dados LISA, iniciada em 1969, mantém sua cobertura temática na área de Biblioteconomia e Ciência da Informação.Método: No estudo utilizou-se o método da bibliometria aplicada para verificar a produção científica indexada.Resultados: Entre os resultados da busca de termos indexados na base de dados LISA, encontraram-se 503 artigos com o termo “...

  15. Plataforma de força instrumentada: uma ferramenta aplicada a estudos de posturologia

    OpenAIRE

    Rodowanski, Ivanoé João

    2011-01-01

    O trabalho aqui apresentado faz uma abordagem das tecnologias envolvidas e descreve o projeto, construção e testes de validação de uma plataforma de força que é um equipamento utilizado por profissionais das áreas de saúde que trabalham com estudo de comportamento biomecânico humano tais como: posturologia, equilíbriometria, estabilometria, etc., como exemplo fisioterapeutas no acompanhamento da reabilitação de pacientes com problemas locomotores, estudos de comportamentos posturais ou anális...

  16. O desenvolvimento da teoria da mente em psicoterapeutas : um estudo empírico

    OpenAIRE

    Lopes, Rodrigo da Cunha Teixeira

    2009-01-01

    Dissertação de mestrado em Psicologia (área de especialização em Psicologia Clínica) Já é bastante reconhecida a importância das características pessoais do psicoterapeuta no processo e resultado da psicoterapia. Por essa razão, justificam-se os estudos voltados para o conhecimento de quem é a pessoa do psicoterapeuta. É também reconhecida a importância que a empatia do psicoterapeuta tem para o processo terapêutico. Notamos estudos que apontam para uma alta associação entre...

  17. Estudo de ligações em estruturas mistas de concreto-madeira

    OpenAIRE

    Elias Antonio Nicolas

    2001-01-01

    Resumo: Esta pesquisa se refere ao estudo do comportamento mecânico de conectores empregados em estruturas mistas de concreto e madeira, estruturas estas normalmente utilizadas em construções civis tais como pontes, pisos e coberturas residenciais. Neste sentido, foi realizado um estudo teórico-experimental sobre os conectores onde se determinou o valor do módulo de deslizamento (K) através de curvas cargas x deslizamentos obtidas a partir ensaios de laboratório. Os corpos-de-prova foram mold...

  18. Um estudo das relações interpessoais em famílias com farmacodependentes

    OpenAIRE

    Silva, Jair Lourenço; Macedo, Rosa Maria S.; Derntl, Alice Moreira; Bergami, Nancy Benedita Berrueso

    2007-01-01

    Este estudo tem como objetivo evidenciar as implicações dos padrões de humor na manutenção do ciclo da drogadicção nos sistemas familiares com dependentes químicos, tendo como pressupostos as correlações entre drogadicção e transtornos de humor. Consideramos importante investigar como os padrões de depressão e mania afetam e são afetados, recursivamente, pelo sistema familiar com membro adicto. Apresentamos um estudo de caso de terapia familiar estrutural e estratégica de uma família com um m...

  19. Prevalência dos sintomas otológicos na desordem temperomandibular: estudo de 126 casos

    OpenAIRE

    Pascoal Maria I. N.; Rapoport Abrão; Chagas José F. S.; Pascoal Maria B.N.; Costa Claudiney C.; Magna Luis Antonio

    2001-01-01

    Introdução: A presença de sintomas otológicos associados à desordem temporomandibular (DTM) é discutida há seis décadas; entretanto, sua etiologia ainda permanece obscura. Forma de estudo: Prospectivo clínico randomizado. Objetivo: Neste estudo foram avaliadas a prevalência de sintomas otológicos na DTM, sua correlação com a dor muscular e a ausência de dentes posteriores. Material e Método: Foram avaliados 126 pacientes portadores de DTM, através de questionário subjetivo dos sintomas, palpa...

  20. GEOSSISTEMAS E CLIMATOLOGIA GEOGRÁFICA: ESTUDOS INTEGRADOS DO CLIMA EM BACIAS HIDROGRÁFICAS

    OpenAIRE

    Wesley da Silva Belizário

    2015-01-01

    Os estudos do ambiente têm sido cada vez mais frequentes, sobretudo aqueles relacionados aos impactos negativos ao ambiente natural, seja no espaço rural ou urbano. Várias contribuições teóricas têm surgido para dar sentido e embasamento para tais análises, principalmente aquelas que tentam relacionar todas as possibilidades presentes no meio, a fim de que se efetive o chamado estudo integrado do ambiente, sendo uma dessas contribuições a teoria dos geossistemas. O presente trabalho tem como ...

  1. Neoplasias oculares em cães e gatos : estudo retrospetivo 2001-2012

    OpenAIRE

    Silva, Beatriz Rosa Fernandes Duarte da

    2013-01-01

    Dissertação de Mestrado Integrado em Medicina Veterinária Apresenta-se um estudo sobre os casos de neoplasias oculares, em cães e gatos, analisados pelo Laboratório de Anatomia Patológica da Faculdade de Medicina Veterinária da Universidade Técnica de Lisboa (FMV-UTL), entre 2001 e 2012. Este estudo pretende contribuir para o conhecimento da prevalência das neoplasias oculares em cães e gatos na região de Lisboa, auxiliando no diagnóstico e na terapêutica de futuros casos de neoplasias ...

  2. Estudo de mercado: "A satisfação dos franchisados em Portugal"

    OpenAIRE

    Fialho, Maria Cristina Grilo

    2013-01-01

    Este relatório de estágio foi realizado no âmbito do Mestrado em Matemática e Aplicações, com especialização em Estatística e Investigação Operacional, da Universidade de Aveiro, e o estágio foi realizado no GEMEO (Gabinete de Estudos de Mercado e Opinião do IPAM). Foram desenvolvidas várias atividades relacionadas com os Estudos de Mercado, e uma dessas atividades é sobre o Franchising, onde se pretende avaliar o perfil e satisfação do Franchisado em Portugal. Para isso, fo...

  3. Estudo da sintomatologia depressiva e ansiosa em crianças com e sem obesidade

    OpenAIRE

    Morgado, Joana Alexandra da Silva

    2013-01-01

    A obesidade infantil constitui um problema de saúde pública, o que motivou a sua classificação pela WHO como “epidemia do seculo XXI”. Como se não bastasse, surge sempre associada ao aumento quer da mortalidade, quer da morbilidade. Este estudo procura perceber em que medida as crianças com obesidade infantil diferem das crianças sem obesidade em termos de sintomatologia depressiva e ansiosa. O desenho do estudo é de natureza transversal e comparativa. A amostra é composta por 85 crianças, co...

  4. Estilos educativos parentais, valores e autoconceito de adolescentes : um estudo transcultural

    OpenAIRE

    Iglesia, Yara Rodrigues de la

    2017-01-01

    Tese de doutoramento, Educação (Psicologia da Educação), Universidade de Lisboa, Instituto de Educação, 2017 O presente estudo teve como objetivo principal investigar, a partir de uma perspectiva transcultural, como se caracterizam os estilos educativos parentais, o autoconceito e os valores na adolescência, como se relacionam estas variáveis entre si e como se diferenciam, em função de variáveis específicas. Em relação à metodologia, foi realizado um estudo quantitativo, de tipo transvers...

  5. Um estudo sobre a esclerose multipla a luz da teoria winnicottiana

    OpenAIRE

    Nogueira, Giuliana Gomes

    2010-01-01

    Esta dissertação busca construir um trajeto que possibilite discutir a Esclerose Múltipla no hall das doenças psicossomáticas tomando como referencial a relação psicossomática, abordada sob a ótica de D. W. Winnicott. Assim, realiza-se um estudo que permite ao leitor conhecer de forma ampla a Esclerose Múltipla detendo-se em seus 4 aspectos históricos; sua etiologia; diagnóstico; tratamentos; prognósticos; aspectos psicológicos. Este estudo se preocupa, ainda, em abordar os conceitos fu...

  6. O impacto dos acontecimentos significativos no processo terapêutico: Um estudo de caso de sucesso

    OpenAIRE

    Corrêa, Andriza; Ribeiro, Eugénia; Costa, Sara

    2016-01-01

    Este estudo teve como objetivo descrever os impactos associados aos acontecimentos significativos, identificados pelo cliente e terapeuta, ao longo de um caso de sucesso clínico. Utilizámos a metodologia de estudo de caso, com um cliente adulto, diagnosticado com perturbação de pânico e acompanhado em terapia comportamental e cognitiva. A versão portuguesa do questionário Helpful Aspects of Therapy foi administrada em todas as sessões para a recolha dos acontecimentos significa...

  7. Estudo químico e biológico de Genipa americana L. (Jenipapo)

    OpenAIRE

    Alves, Jovelina Samara Ferreira

    2014-01-01

    Genipa americana Linnaeus (Rubiaceae), ocorre amplamente na regiãoNordeste e em outras regiões do Brasil, como também em outros países. Sob oponto de vista medicinal, a espécie é usada pela população para diferentes finscomo: catártico, antidiarréico, antigonorréico, antiulceroso, analgésico, emcasos de sífilis, anemia, icterícia, asma, dentre outros. Devido ao reconhecidouso popular e a escassez de estudos químicos e farmacológicos, o principalobjetivo deste estudo foi caracterizar os marcad...

  8. Cultura e enoturismo: um estudo da Região Demarcada do Douro

    OpenAIRE

    Brambilla, Adriana

    2015-01-01

    O presente trabalho propôs-se a estudar as relações entre a cultura e o enoturismo praticado nas quintas da Região Demarcada do Douro. Neste sentido, partimos em busca de um referencial teórico que pudesse nos orientar na compreensão das relações entre cultura e turismo a partir das questões identitárias e, por isso, procuramos investigar epistemologias de diálogo entre os Estudos Culturais e os Estudos do Turismo. Após a primeira fase da pesquisa bibliográfica, procedemos a uma pesquisa empí...

  9. COMPORTAMENTO DO CONSUMIDOR ENDIVIDADO: UM ESTUDO EXPLORATÓRIO COM INDIVÍDUOS DE BAIXA RENDA

    OpenAIRE

    Celso Augusto de Matos; Kátia Bonfanti; Frederike Monika Budiner Mette

    2015-01-01

    O presente estudo teve como objetivo geral analisar os fatores associados ao superendividamento de consumidores de baixa renda. Foi realizado um estudo exploratório, de cunho qualitativo, por meio de entrevistas individuais com consumidores das classes D e E. Dentre os principais fatores associados ao acúmulo de dívidas pelos entrevistados, destacam-se as compras por impulso, o pagamento do valor mínimo das faturas do cartão de crédito, o uso de financiamentos e empréstimos para aquisição de ...

  10. Potencial antioxidante do baru (dipteryx alata vog.) : um estudo in vitro e in vivo

    OpenAIRE

    Marins, Alinne Martins Ferreira

    2012-01-01

    O efeito protetor de alimentos de origem vegetal tem sido atribuído a presença de compostos bioativos encontrados em todas as partes da estrutura das plantas. Estudos anteriores retrataram uma alta concentração de sais minerais, taninos e ácido fítico na amêndoa de baru (Dipteryx alata Vog.), fruta típica do Cerrado brasileiro. O presente estudo investigou a atividade antioxidante (AA) dos extratos aquoso e acetato de etila da amêndoa do baru in vitro bem como os efeitos do consumo desta amên...

  11. MARCAS DE ORALIDADE NO CADERNO KZUKA – UM OLHAR SOB A PERSPECTIVA DOS ESTUDOS CULTURAIS

    Directory of Open Access Journals (Sweden)

    Angela Dillmann Nunes Bicca

    2016-04-01

    Full Text Available Neste estudo, buscamos compreender como as marcas de oralidade presentes no jornalismo estão implicadas na constituição de identidades culturais juvenis. Sob a perspectiva dos Estudos Culturais, discutimos, a partir da abordagem discursiva da Representação Cultural, os modos como o Caderno Kzuka – periódico semanal do jornal gaúcho Zero Hora – promove uma forma de Pedagogia Cultural, ao incorporar expressões e elementos tipicamente juvenis ao texto escrito no semanário.

  12. Efeitos antimicrobianos de cetamina em combinação com propofol: um estudo in vitro

    OpenAIRE

    Begec, Zekine; Yucel, Aytac; Yakupogulları, Yusuf; Erdogan, Mehmet Ali; Duman, Yucel; Durmus, Mahmut; Ersoy, M. Ozcan

    2013-01-01

    EXPERIÊNCIA E OBJETIVOS: Cetamina e propofol são os anestésicos gerais que também exibem efeitos antimicrobianos e promotores do crescimento microbiano, respectivamente. Embora esses agentes sejam frequentemente aplicados em combinação durante o uso clínico, não há dados sobre seu efeito total no crescimento microbiano na administração combinada. Nesse estudo, investigamos o crescimento de alguns microrganismos em uma mistura de cetamina e propofol. MÉTODO: Nesse estudo, utilizamos cepas padr...

  13. Sindrome de Landau Kleffner aspectos clinicos, eletrencefalograficos e psicossociais : estudo de seguimento a longo prazo

    OpenAIRE

    Marcos Henrique Coelho Duran

    2008-01-01

    Resumo: Objetivos: A Síndrome de Landau-Kleffner (SLK) é uma entidade rara, caracterizada por epilepsia e afasia. Ocorre em crianças com aquisição normal da linguagem, geralmente, entre 3 e 7 anos de idade. A evolução a longo prazo é controversa e pouco estudada. O presente estudo tem como objetivo verificar a evolução em uma série de sujeitos com diagnóstico de SLK, enfocando os aspectos clínicos, eletrencefalográficos, psicossociais e da qualidade de vida. Métodos: Trata-se de um estudo tra...

  14. Estudo de ligas de ouro por XRF e SEM-EDS

    OpenAIRE

    Coxito, Afonso Maria Teixeira

    2016-01-01

    Serve o presente estudo para testar uma metodologia para análise de ligas metálicas, em particular ligas de ouro, utilizando as técnicas de fluorescência de raios-X e microscopia electrónica de varrimento. No contexto do presente estudo, foi identificada a composição das folhas de ouro utilizadas em obras de arte. As técnicas laboratoriais utilizadas foram: Microscopia óptica; Espectroscopia de fluorescência de raios-X com dispersão de energia (EDXRF); Microscopi...

  15. Índice de mortalidade durante procedimentos anestésicos: estudo retrospectivo (1996-2006)

    OpenAIRE

    Corrêa, André Luís; Oleskovicz, Nilson; Moraes, Aury Nunes de

    2009-01-01

    O objetivo deste estudo é reportar a incidência de óbitos anestésico-cirúrgicos durante um período de 11 anos (1996-2006), no qual não eram realizados exames complementares e/ou laboratoriais previamente ao encaminhamento para anestesia. Dessa forma, realizou-se um estudo retrospectivo, avaliando-se as fichas anestésicas utilizadas durante os procedimentos realizados no Hospital de Clínica Veterinária (HCV) da Universidade do Estado de Santa Catarina (UDESC). Analisaram-se 7.012 procedimentos...

  16. ESTUDO DE ETNOECODESIGN DE IMAGENS SACRAS EM GESSO DANIFICADAS E DESCARTADAS PELO USUÁRIO. ESTUDO DE MODELO SUSTENTÁVEL DE NICHO DE DESCARTE.

    Directory of Open Access Journals (Sweden)

    Paulo Sergio de Sena

    2016-10-01

    Full Text Available O Etnoecodesingn trata-se de um estudo interdisciplinar onde o dialogo envolve os critérios culturais, sociais e ambientais. Os componentes etnoecológicos do estudo das Imagens Sacras de Gesso se materializam no grupo de artesãos tradicionais locais (mais de 100 anos de exploração da técnica e recurso natural gesso. Na produção de imagens sacras, como souvenir, em Aparecida- SP, que agrega processos e materiais que contribuem significativamente para contaminação do ambiente, durante a pré-produção, produção, pós-produção e descarte, principalmente quando houve constatação, no projeto anterior, de matéria prima contaminada por metais pesados. Este trabalho trata dos possíveis impactos causados pelo descarte das imagens, uma vez danificadas, diretamente no ambiente por conta de uma prática cultural denominada de “Santa Cruz” e vem contribuir para o estudo comparado dos atuais modelos de nicho de descarte e a proposta de um modelo mais sustentável para a Santa Cruz.

  17. Debates críticos: os Estudos de Segurança e o futuro dos Estudos da Paz e dos Conflitos 10.5102/uri.v11i2.2526

    Directory of Open Access Journals (Sweden)

    André Barrinha

    2013-11-01

    Full Text Available Irremediavelmente ligados pela proximidade dos respetivos objetos de estudo, Estudos de Segurança e Estudos da Paz e dos Conflitos constituíram-se e evoluíram ao longo da Guerra Fria como disciplinas antagónicas. Essa divisão operou-se sobretudo na Europa, onde Galtung e seus discípulos encaminharam o estudo das questões da guerra e da paz para uma agenda claramente normativa e crítica, enquanto o estudo das questões de segurança se centrava numa abordagem orientada para o policy-making. Como é defendido neste artigo, o final do conflito bipolar correspondeu, até certo ponto, a uma inversão de papéis, com os Estudos da Paz e dos Conflitos acomodados a um empirismo desprovido de normatividade e os Estudos de Segurança, pelo menos na Europa, a abrirem-se a novas abordagens de cariz mais crítico. É aqui sugerido que esta inversão contém, em si, importantes lições para os Estudos da Paz e da Segurança, nomeadamente sobre a centralidade da teoria na definição de uma nova agenda crítica que permita uma aproximação entre as duas disciplinas.

  18. Antimalarial sesquiterpene lactones from oncosiphon piluliferum

    CSIR Research Space (South Africa)

    Pillay, P

    2006-02-01

    Full Text Available to the marked instability of the germacranolide (1), the structural elucidation and bioassaying was conducted on the acetylated derivative (1a). Mosher’s method was applied to determine the absolute stereochemistry of the major compound (5), identifying..., Botanic Gardens Road, Durban, 4001 3Department of Pharmacology, University of Cape Town, Observatory, 7925 4Department of Chemistry, University of Pretoria, Pretoria, 0002 1Email: ppillay@csir.co.za K-4570 [ww w .kashangroup.com ] Figure 1: X...

  19. Systemic allergic dermatitis caused by sesquiterpene lactones

    DEFF Research Database (Denmark)

    Paulsen, Evy

    2017-01-01

    allergens such as those of lettuce. Other Compositae species suspected of causing systemic reactions are artichoke, mugwort, yarrow, dandelion, feverfew, and elecampane. Some Compositae vegetables and teas, such as lettuce and chamomile tea, may induce systemic reactions through both humoral and cell...

  20. Three new sesquiterpene lactones from Inula britannica.

    Science.gov (United States)

    Zhang, Xu-Feng; Du, Jing-Ling; Ren, Jie; Ye, Feng-Mei; Xie, Yang-Guo; Cheng, Xiang-Rong; Yan, Shi-Kai; Jin, Hui-Zi

    2015-01-01

    One new 1,10-secoeudesmanolide (1), two eudesmanolides (2 and 3), together with nine known compounds (4-12) were isolated from the aerial parts of Inula britannica. The structures of the new compounds were elucidated by detailed spectroscopic analysis, including HRESIMS and 2D-NMR spectroscopic method. In addition, compounds 1-4 were tested for their inhibitory effects against LPS-induced NO production in RAW264.7 macrophages.

  1. A NEW BENZOPYRANYL SESQUITERPENE ISOLATED FROM ...

    African Journals Online (AJOL)

    dell

    Isoschefflerin: Prenylated Chalcones and other Constituents of Uvaria scheffleri,. Phytochemistry, 29:1261-1264. Nkunya MHH, Weenen H, Bray DH, Mgani. QA, Mwasumbi LB 1991 Antimalarial. Activities of Tanzanian Medicinal Plants and their Active Constituents, Planta. Med. 57:341-343. Okokon JE, Ita BN, Udokpoh AE ...

  2. Uvarisesquiterpene D: A New Benzopyranyl Sesquiterpene Isolated ...

    African Journals Online (AJOL)

    ... and NMR) and comparison of its spectral data with the known related compounds uvarisesquiterpene B and selin-11(12)-4α-ol. The latter compound was co-isolated with the new natural product as well as caryophyline oxide and lucidine. Key words: Annonaceae, uvaria, bezopyranylsesquiterpenes, uvarisesquiterpenes ...

  3. COMO OLHAMOS E SOMOS OLHADOS PELAS IMAGENS? ESTUDOS CRÍTICOS DOS ARTEFATOS DA CULTURA VISUAL

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    João Paulo Baliscei

    2015-06-01

    Full Text Available A intensidade com que os indivíduos pós-modernos se relacionam com a visualidade incentiva-nos a refletir sobre os modos pedagógicos de estudar os artefatos culturais do cotidiano, chamados de artefatos da Cultura Visual. Neste artigo nosso objetivo é investigar as aproximações e convergências entre pesquisadores/as dos Estudos Culturais e o Estudo da Cultura Visual no que diz respeito aos estudos de artefatos visuais. Para isso, desenvolvemos uma pesquisa bibliográfica em que discutimos sobre quatro perspectivas que demonstram posicionamentos pedagógicos sobre a Cultura Visual. Além disso, respaldamo-nos sobre a perspectiva autorreflexiva para estudar imagens e discursos oferecidos por meio da boneca Barbie. Em nossas considerações, evidenciamos oito estratégias de estudos de artefatos visuais que comungam com os princípios das vertentes teóricas citadas.

  4. Auditoria interna de sistema de gestão ambiental: estudo de caso no Verdegreen Hotel

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    Adriano Lucena da Silva

    2017-06-01

    Full Text Available Esta pesquisa analisa as contribuições e os desafios associados às auditorias internas no Verdegreen Hotel, empreendimento hoteleiro de grande destaque na cidade de João Pessoa-PB, selecionado como unidade de estudo por dispor de um Sistema de Gestão Ambiental (SGA ISO 14001 implantado e certificado. Este estudo trata de uma pesquisa descritiva, baseada em um estudo de caso qualitativo. As técnicas utilizadas na metodologia foram: levantamento bibliográfico sobre as normas ISO que abordam as temáticas SGA e auditoria; questionários aplicados aos membros do comitê verde; e análise documental do manual do SGA. Para este estudo, três categorias foram selecionadas: i instrumentos, ii procedimentos e iii recursos humanos. A partir da análise de conteúdo das respostas dos questionários aplicados e do manual do SGA, foi constatado que as auditorias internas do Verdegreen Hotel ocorrem baseadas em métodos bem definidos de coleta e tratamento de informações e que o hotel mantém programas de capacitação para seus colaboradores. Com base nos resultados da pesquisa, tornou-se possível compreender como as questões relacionadas à aplicação da auditoria interna são oportunidades de aprimoramento do SGA implantado.

  5. GEOSSISTEMAS E CLIMATOLOGIA GEOGRÁFICA: ESTUDOS INTEGRADOS DO CLIMA EM BACIAS HIDROGRÁFICAS

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    Wesley da Silva Belizário

    2014-12-01

    Full Text Available Os estudos do ambiente têm sido cada vez mais frequentes, sobretudo aqueles relacionados aos impactos negativos ao ambiente natural, seja no espaço rural ou urbano. Várias contribuições teóricas têm surgido para dar sentido e embasamento para tais análises, principalmente aquelas que tentam relacionar todas as possibilidades presentes no meio, a fim de que se efetive o chamado estudo integrado do ambiente, sendo uma dessas contribuições a teoria dos geossistemas. O presente trabalho tem como objetivos refletir sobre a teoria geossitêmica, seu surgimento, seus referenciais, suas bases, e suas contribuições para a análise e gestão de bacias hidrográficas. Da mesma forma, conceituar climatologia geográfica, relacionar com a abordagem geossitêmica e conflitar esses conceitos no sentido de seguir para a determinação das bacias hidrográficas como áreas propícias aos estudos em climatologia, tendo em vista que as bacias são unidades que englobam todos os fenômenos possíveis, espaciais, temporais, físicos, naturais, sociais, territoriais e econômicos. Nesse aspecto, as bacias hidrográficas são ambientes completos, do ponto de vista físico e social para estudos climatológicos.

  6. OS SISTEMAS AGROFLORESTAIS COMO ALTERNATIVA ECONÔMICA EM PEQUENAS PROPRIEDADES RURAIS: ESTUDO DE CASO

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    Mário Jorge Campos dos Santos

    2002-05-01

    Full Text Available Os Sistemas Agroflorestais (SAFs constituem-se em uma alternativa de uso da terra para aliar a estabilidade do ecossistema visando à eficiência e otimização de recursos naturais na produção de forma integrada e sustentada. O objetivo deste estudo de caso foi avaliar a viabilidade econômica de um sistema agroflorestal localizado na região do Pontal do Paranapanema. O SAF em estudo é formada por espécies agrícolas: milho (Zea maiz, feijão guandu (Cajanus cajan, carioquinha, (Vigna unguiculata, preto (Phaseolus vulgaris e mandioca (Manihot esculenta com espécies florestais: Eucalyptus citriodora e Eucalyptus camaldulensis. A rentabilidade econômica do sistema foi mensurada, utilizando Valor Presente Líquido (VPL, Razão Benefício/Custo (RB/C para as culturas agrícolas anuais e Valor Esperado da terra (VET para os componentes madeireiros. Para tais cálculos foi escolhida uma taxa de desconto de 6%. O estudo demonstrou que o sistema agroflorestal apresentou rentabilidade econômica positiva, e que podem ser adotados por pequenos produtores. Assim, pode-se concluir que os sistemas agroflorestais são economicamente viáveis para pequenos produtores rurais trabalhando nas mesmas condições apresentadas neste estudo.

  7. IMPACTOS MICROCLIMÁTICOS DO DESENHO URBANO: ESTUDOS REALIZADOS EM CURITIBA

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    Eduardo KRUGER

    2011-04-01

    Full Text Available O artigo busca compreender as relações entre atributos da geometria urbana e alterações no microclima. São apresentados dois estudos distintos conduzidos na mesma área central de Curitiba, PR, a partir de medições de campo realizadas em 2009 e de simulações de clima urbano no software ENVI-met. Os estudos, embora utilizem a mesma campanha de coleta de dados, possuem objetivos e metodologias diferentes. O primeiro estudo foca no impacto da geometria urbana, na temperatura ambiente e nos níveis de conforto em ruas de pedestre no período diurno, sendo o fator de visão do céu utilizado como indicador da complexa geometria urbana. O segundo estudo trata do impacto da orientação das vias em relação ao vento dominante, quanto às taxas de ventilação resultantes (velocidade do ar e distribuição espacial, visando à dispersão de poluentes gerados pelo tráfego, no contexto urbano. Os resultados auferidos evidenciam a influência da forma urbana para a determinação do conforto térmico em ruas de pedestres, restringindo-se a um período diurno específico, e para a criação de cenários de poluição.

  8. Os sistemas agroflorestais como alternativa econômica em pequenas propriedades rurais: estudo de caso.

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    Mário Jorge Campos dos Santos

    2010-08-01

    Full Text Available Os Sistemas Agroflorestais (SAFs constituem-se em uma alternativa de uso da terra para aliar a estabilidade do ecossistema visando à eficiência e otimização de recursos naturais na produção de forma integrada e sustentada. O objetivo deste estudo de caso foi avaliar a viabilidade econômica de um sistema agroflorestal localizado na região do Pontal do Paranapanema. O SAF em estudo é formada por espécies agrícolas: milho (Zea maiz, feijão guandu (Cajanus cajan, carioquinha, (Vigna unguiculata, preto (Phaseolus vulgaris e mandioca (Manihot esculenta com espécies florestais: Eucalyptus citriodora e Eucalyptus camaldulensis. A rentabilidade econômica do sistema foi mensurada, utilizando Valor Presente Líquido (VPL, Razão Benefício/Custo (RB/C para as culturas agrícolas anuais e Valor Esperado da terra (VET para os componentes madeireiros. Para tais cálculos foi escolhida uma taxa de desconto de 6%. O estudo demonstrou que o sistema agroflorestal apresentou rentabilidade econômica positiva, e que podem ser adotados por pequenos produtores. Assim, pode-se concluir que os sistemas agroflorestais são economicamente viáveis para pequenos produtores rurais trabalhando nas mesmas condições apresentadas neste estudo.

  9. APLICAÇÃO DA FERRAMENTA FMEA: ESTUDO DE CASO EM UMA EMPRESA DO SETOR DE

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    Leandro Cantorski da Rosa

    2007-06-01

    Full Text Available Este trabalho apresenta um estudo de caso em que se implantou a ferramenta Failure Modes and Effects Analysis (FMEA emuma empresa atuante no segmento de transporte terrestre e aéreo de passageiros e cargas. O objetivo deste estudo de caso foideterminar, com a ferramenta FMEA, ações que minimizem ou eliminem modos de falha em potencial em um dos desdobramentos deserviços prestados pela empresa. O ponto específico onde se implantou a ferramenta foi na gestão dos pneus dos veículos de transporteterrestre de passageiros, pois falhas relacionadas a este componente aumentam os custos de manutenção da empresa e tendem a gerarinsatisfações aos clientes. Também foram utilizadas, incorporadas à ferramenta FMEA, outras ferramentas da qualidade como oBrainstorming, Diagrama de Ishikawa e Gráfico de Pareto. Os resultados do trabalho foram alcançados ao se determinar ações quetrazem consigo o objetivo principal deste estudo, isto é, o aumento de confiabilidade e qualidade do serviço prestado. Deste modo, arealização do presente estudo de caso proporcionou um maior entendimento acerca da temática proposta, além de mostrar aimportância da gestão da qualidade nos dias atuais e frente as crescentes exigências dos clientes.

  10. Políticas de inclusão escolar: um estudo sobre a classe hospitalar no Brasil

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    Ricardo Antônio Gonçalves Teixeira

    2017-09-01

    Full Text Available Este estudo objetiva apresentar um quadro teórico, metodológico e documental das produções científicas sobre classes hospitalares no Brasil. Caracteriza-se como estudo bibliográfico, valendo-se do método de Revisão Sistemática. Como resultados, apresenta a predominância da pesquisa qualitativa como metodologia de estudo; a proveniência de publicações vinculadas a instituições federais de ensino; recorrência de estudos focados em atividades de formação docente e sondagem sobre percepção de acompanhantes a respeito de atendimentos pedagógicos realizados no âmbito hospitalar. Contribui com uma matriz de referência teórico-documental, servindo de base para estudos sobre o tema.

  11. Religiosidade e problemas com o álcool: um estudo de caso

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    Marlon Xavier

    Full Text Available Este estudo buscou analisar a relação entre os fatores problemas com o álcool e religiosidade. Para tanto, foi utilizado um estudo de caso único, composto por narrativas biográficas e sonhos do sujeito, um homem de mais de 50 anos. A partir desse material, buscou-se verificar como os fatores se manifestavam consciente e inconscientemente, seguindo a teoria e a metodologia de interpretação de C. G. Jung. Conscientemente, a religiosidade manifestou-se através da crença no espiritismo e no que Jung chama de religio; inconscientemente, através de uma religiosidade que chamamos de dionisíaca. O estudo demonstra que as diferentes formas de religiosidade manifestas pelo sujeito apresentaram relações complexas com os problemas com o álcool - desde incitação ao beber e acirramento dos problemas relacionados até possibilidades de resolução dos problemas. As relações do sujeito com os valores coletivos que incentivam o consumo de álcool também foram objeto de estudo. Conclui-se que a religiosidade, de maneira geral, contribuiu para uma relativização na percepção desses valores, fortalecendo uma compreensão mais individual do problema com o álcool. O estudo propõe, ainda, maior atenção ao fator religiosidade na pesquisa sobre álcool.

  12. Estudos transcriptômicos no contexto da conectividade perturbada em esquizofrenia

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    Andrea Schmitt

    2013-01-01

    Full Text Available Esquizofrenia é uma severa doença neurobiológica com fatores genéticos e ambientais desempenhando um papel na fisiopatologia. Diversas regiões cerebrais têm sido implicadas no processo da doença e estão conectadas em complexos circuitos neuronais. Nos níveis molecular e celular, a conectividade afetada entre essas regiões, envolvendo mielinização disfuncional dos axônios neuronais, bem como as alterações no nível sináptico e metabolismo energético levando a distúrbios na plasticidade sináptica, são os maiores achados em estudos post-mortem. Estudos de microarranjos investigando a expressão gênica contribuíram para os achados de alterações em vias complexas em regiões cerebrais relevantes na esquizofrenia. Além disso, estudos utilizando microdissecção e captura a laser permitiram a investigação da expressão gênica em grupos específicos de neurônios. Entretanto, deve ser mantido em mente que em estudos post-mortem, confusos efeitos de medicação, qualidade de RNAm, bem como capacidade de mecanismos regenerativos neuroplásticos do cérebro em indivíduos com história de vida de esquizofrenia, podem influenciar o complexo padrão de alterações no nível molecular. Apesar dessas limitações, estudos transcriptômicos livres de hipóteses em tecido cerebral de pacientes esquizofrênicos oferecem uma possibilidade única para aprender mais sobre os mecanismos subjacentes, levando a novas ópticas da fisiopatologia da doença.

  13. INTRA-EMPREENDEDORISMO E DIMENSÃO CULTURAL: UM ESTUDO COM TRABALHADORES DE UMA EMPRESA CEARENSEl: um estudo com trabalhadores de uma empresa cearense

    OpenAIRE

    Artilene Barros Rabelo Estevam

    2012-01-01

    Trata-se de um estudo sobre práticas de intra-empreendedorismo em um grupo de trabalhadores de uma empresa cearense, considerada de grande porte, com atuação nacional e internacional, localizada na região metropolitana de Fortaleza. Os objetivos da pesquisa foram identificar e descrever práticas intra-empreendedoras; relacioná-las com o comportamento da alta direção; relacionar o comprometimento dos colaboradores com essas práticas e identificar o estilo de liderança predominante na empresa. ...

  14. Estudo sobre os Processos de Recrutamento e Seleção em Agroindústrias: uma análise em dois estudos de casos

    OpenAIRE

    Araujo, Geraldino Carneiro; Dias, Edilaine Mendes

    2008-01-01

    O objetivo do trabalho é analisar e avaliar as formas de recrutar e selecionar pessoas, visto que os recursos humanos são fatores importantíssimos para o funcionamento de qualquer organização. Esse estudo de caso foi feito em duas agroindústrias verificando o seu processo de recrutamento e seleção de pessoal. O tipo de pesquisa é descritivo que expõe características de determinada população ou de determinado fenômeno. A análise foi feita em cima das respostas obtidas na entrevista das duas em...

  15. Computador-internet nas Práticas de Estudo Contemporâneas: uma Pesquisa com Estudantes de Psicologia

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    Beatriz Sancovschi

    2015-03-01

    Full Text Available O artigo objetiva investigar como está sendo realizado o estudo na contemporaneidade. Segue dois caminhos. Inicialmente opera uma inflexão no campo dos estudos sobre o estudo. Ao invés de conceber o estudo como entidade objetiva e naturalizada propõe, com a ajuda das abordagens construtivistas da cognição, como as de J. Piaget, L.Vigotski, F. Varela e H. Maturana e dos trabalhos de M. Foucault e R. Chartier, analisar o problema a partir da noção de práticas de estudo. O estudo passa a ser resultado das ações concretas dos estudantes, deixando de ser concebido como atividade mental supostamente invariante, voltada para aquisição de um saber. Em seguida, apresenta e discute uma pesquisa de campo, na qual foram entrevistados 15 estudantes de Psicologia do primeiro ao nono período de quatro instituições de ensino superior do município do Rio de Janeiro, sendo duas públicas e duas privadas. As entrevistas seguiram a técnica da entrevista de explicitação. A conclusão aponta para a importância do uso do computador-internet nas práticas de estudo contemporâneas e analisa alguns de seus efeitos, como a grande quantidade de informação, o estudo em circulação, a fragmentação e composição de partes e a coexistência do estudo com o texto digital e impresso.

  16. Construindo pontes: a prática da interdisciplinaridade. Estudo PopTrans: um estudo com travestis e mulheres transexuais em Salvador, Bahia, Brasil

    Directory of Open Access Journals (Sweden)

    Ines Dourado

    Full Text Available Resumo: Pessoas trans, incluindo travestis, mulheres transexuais, apresentam taxas desproporcionalmente elevadas de HIV/AIDS em comparação com o restante da população. Entretanto, são poucos os estudos quantitativos/qualitativos com pessoas trans no Brasil. Assim, uma equipe de pesquisadores de diferentes áreas desenvolve um projeto de pesquisa interdisciplinar com o objetivo de conhecer as condições e os modos de vida, e ao mesmo tempo investigar fatores determinantes da infecção pelo HIV, sífilis e hepatites B e C entre travestis e mulheres transexuais. Neste artigo, pretende-se descrever a experiência de implantação e desenvolvimento do estudo com uma abordagem etnoepidemiológica em Salvador, Bahia, Brasil. O mapeamento da população começou com uma pesquisa formativa que foi crucial para orientar o inquérito epidemiológico. A produção de dados etnoepidemiológicos é um desafio cotidiano para os pesquisadores, produzindo uma série de reflexões sobre os limites de nossos conceitos e categorias para traduzir a diversidade de práticas e experiências das participantes da pesquisa.

  17. Estudo comparado entre os programas de transferência condicionada de renda no Brasil e México

    OpenAIRE

    Marcelo Driusso

    2010-01-01

    A dissertação Estudo comparado entre os Programas de Transferência Condicionada de Renda no Brasil e no México tem como objetivo aprofundar o estudo dos dois principais programas desse tipo nos países citados a fim de identificar suas virtudes e limitações. Considerando que na América Latina os desafios para redução da pobreza e da desigualdade de renda continuam sendo enormes, com necessidade de ampla discussão entre sociedade civil e governos para superação dessas barreiras, os estudos dess...

  18. Narrativas pessoais midiatizadas: uma proposta para o estudo de práticas orientadas pela mídia

    OpenAIRE

    Escosteguy, Ana Carolina; PUCRS

    2011-01-01

    Os estudos de mídia têm estado dominados por explícitas ou implícitas noções de comunicação linear, isto é, pela ideia de que os textos midiáticos movem-se dos produtores aos consumidores. Em princípio, os estudos culturais rejeitam tal concepção unidirecional e compartimentada. No entanto, grande parte da pesquisa empírica, associada a esse campo, ora privilegia o texto, ora as audiências. Com o objetivo de descartar essa fragmentação e direcionalidade, é explorada a perspectiva de estudo de...

  19. O uso da eletroglotografia, eletromiografia, espectografia e ultrassom nos estudos de fala - revisão teórica

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    Fernanda Wiethan

    2015-03-01

    Full Text Available O emprego de novas tecnologias na avaliação e terapia de fala a partir de uma revisão de artigos publicados nos últimos 5 anos é o tema deste estudo, que tem por objetivo realizar uma revisão bibliográfica dos estudos nacionais e internacionais que utilizaram os recursos: eletroglotografia, espectrografia, ultrassonografia e eletromiografia na avaliação e terapia das alterações de fala. Existe um crescente interesse da inserção desses recursos nos estudos de fala, contudo, os trabalhos existentes que os correlacionam ainda são escassos.

  20. Lésbicas, família de origem e família escolhida: um estudo de caso

    OpenAIRE

    Cancissu, Cynthia Regina Pemberton

    2007-01-01

    O presente estudo discute a rede de relacionamento lésbico, a presença de uma família escolhida e como se constrói esta rede de relacionamentos. Para chegar a uma possível compreensão deste fenômeno, realizou-se uma pesquisa qualitativa com delineamento de estudo de caso e entrevista semi-estruturada. O caso escolhido para o estudo foi o de uma mulher homossexual assumida, com mais de 30 anos de idade e com a vivência de mais de um relacionamento amoroso homossexual signific...

  1. Os significados do trabalho para a juventude: um estudo sócio-histórico com adolescentes ricos

    OpenAIRE

    Oliveira, Liara Rodrigues de

    2011-01-01

    Este estudo teve como interesse, investigar a relação que o jovem adolescente rico possui com o trabalho e a escolha profissional. A escolha por essa população como objeto de estudo, partiu do trabalho de orientação profissional desenvolvido com adolescentes de escolas particulares que possuem uma condição socioeconômica e cultural favorecida que nos permitiu um contato espontâneo com os sentidos do trabalho gerando interesse pelo estudo. Como o jovem entende o trabalho, o que ...

  2. A oralidade no ensino de língua portuguesa: um estudo da fala como prática discursiva

    OpenAIRE

    Morales, Vanessa Paternostro

    2009-01-01

    Esta dissertação de mestrado, realizada no Programa de Estudos Pós- Graduados da Pontifícia Universidade Católica de São Paulo, faz um estudo da organização e funcionamento da oralidade como modalidade de língua e da importância de sua aplicação pedagógica. Tradicionalmente, a escola tem privilegiado a escrita como objeto de estudo e de ensino. Nos últimos anos, no entanto, o desenvolvimento de linhas de investigação lingüística tem possibilitado maior reconhecimento da oral...

  3. Unidade de estudos de mastites em pequenos ruminantes: estudos em ovinos de regime extensivo Small ruminant mastitis unity: studies on grazing ewes

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    M.C. Queiroga

    2007-01-01

    Full Text Available A “Unidade de Estudo de Mastites em Pequenos Ruminantes”, formalmente constituída no âmbito do Programa Nacional de Re-equipamento Científico, da Fundação para a Ciência e a Tecnologia, vem dar corpo ao trabalho de colaboração de diversas equipas de investigação que se têm debruçado sobre a problemática das infecções intramamárias em pequenos ruminantes, nos seus aspectos de saúde animal, saúde pública veterinária e qualidade e segurança alimentar. Tem como principal objectivo criar condições para o desenvolvimento de trabalhos de investigação e divulgação científica que contribuam para reduzir a prevalência de mastites em pequenos ruminantes e assim melhorar a produção e a qualidade do leite, favorecendo produtores de leite, produtores de queijo e consumidores. Entre os trabalhos já realizados pela equipa científica, destacam-se o isolamento e identificação de agentes etiológicos de mastite em ovelhas, o estudo de factores de virulência nas bactérias mais relevantes, o estudo dos mecanismos de infecção e a resposta imunológica local e sistémica do hospedeiro e outros estudos na área da epidemiologia. O estudo da etiologia e da fisiopatologia da mastite ovina, com o objectivo de compreender a modulação da resposta imunitária da glândula mamária, poderá contribuir para o desenvolvimento de métodos de controlo com base na estimulação da resposta imunitária, alternativos ao uso de antibióticos.The “Small Ruminant Mastitis Unit”, formally constituted in the scope of the Programa Nacional de Re-equipamento Científico, Science and Technology Foundation, gathers the collaborative work of several research groups that have been addressing the issue of small ruminants intramammary infections, related to animal health, veterinary public health and food safety and quality. Its main purpose is to generate an adequate environment for research and scientific communication that may contribute to

  4. Gênero e feminismo no Brasil: uma análise da Revista Estudos Feministas Gender and feminist studies in Brazil: an analysis of Revista Estudos Feministas

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    Debora Diniz

    2004-12-01

    Full Text Available A Revista Estudos Feministas (REF é uma das mais importantes publicações sobre gênero e feminismo no Brasil. Este artigo descreve o perfil das autoras e dos temas dos artigos publicados na REF de 1992 até 2002. Foram analisados os títulos, os resumos e as palavras-chaves dos artigos avulsos e os temas dos dossiês, em um total de 20 editoriais, 105 artigos avulsos e 20 dossiês. A classificação e indexação temática do material seguiram o vocabulário controlado do "Tesauro para Estudos de Gênero e sobre Mulheres", da Fundação Carlos Chagas. Os resultados da análise mostram que 95% das autoras são mulheres e que 54% são oriundos das Ciências Sociais. Há artigos em todas as áreas temáticas previstas no Tesauro, exceto em "Ciência e Tecnologia" e "Educação", havendo uma concentração de publicações nas áreas temáticas "Ciências Sociais e Cultura" (26%, "Linguagem, Literatura, Religião e Filosofia" (17% e "História e Mudança Social" (17%. Os assuntos mais discutidos foram nesta ordem: divisão sexual do trabalho, análise literária, reprodução e teoria feminista. A análise mostra ainda que o trabalho de pesquisa em gênero e feminismo é solitário, havendo poucos artigos em co-autoria (12% e a metodologia de pesquisa preferencial é a qualitativa, em 92%. A freqüência com que a mesma autora publica na REF é bastante baixa, havendo uma alta rotatividade de autoras.. A expectativa é que os resultados desta análise facilitem a definição de prioridades temáticas, favoreçam a criação de estratégias de pesquisa em áreas pouco exploradas, cooperando para a consolidação do trabalho educativo e político da revista, o que fortalecerá os estudos e pesquisas em gênero e feminismo no Brasil.Revista Estudos Feministas (REF is one of the most important gender and feminist journals in Brazil. This article describes the author background and the subjects of the articles published in REF from 1992 to 2002. It

  5. BRASIL E CHILE: UM ESTUDO COMPARADO SOBRE O RECONHECIMENTO DA UNIÃO ESTÁVEL

    OpenAIRE

    Nascimento, Grasiele Augusta Ferreira; Pardo, Francisco Javier Talep

    2017-01-01

    O presente trabalho tem como objetivo realizar um estudo comparado sobre o reconhecimento da união estável no Brasil e no Chile, através da análise da Constituição da República Federativa do Brasil, do Código Civil e jurisprudência dos tribunais brasileiros, e da Lei 20.830, de 21/04/2015, que criou o Acordo de União Civil no Chile. O estudo é realizado por meio do método teórico-documental, numa perspectiva comparada, no intuito de perceber as semelhanças e diferenças do instituto da união e...

  6. Marketing 3.0 : aplicação no mercado : estudo de caso da Nissan

    OpenAIRE

    Bastos, Maria Catarina de Albuquerque Franco

    2014-01-01

    A ciência do marketing está a entrar numa nova Era à qual vários autores dão o nome de marketing 3.0. Optámos por adotar este termo em todo o nosso relatório, a fim de estudar esta nova aplicação do marketing em geral e dentro da empresa Nissan. O presente estudo tem como objetivo a compreensão das recentes evoluções e motivações que deram origem a esta nova abordagem. A nossa investigação ambiciona ainda revelar através do estudo de caso da Nissan, se a empresa está consciente da existência ...

  7. Estudo morfométrico de rins de primatas Callithrix jacchus em cativeiro

    OpenAIRE

    Rafael Garabet Agopian

    2010-01-01

    Atualmente, os primatas de pequeno e médio porte, em especial os sagüis, têm grande destaque em estudos experimentais, pois são usados frequentemente como modelo para estudos comparativos de várias doenças em humanos, entretanto vários aspectos básicos de sua anatomia ainda estão por serem estudados. Com esta motivação, rins de 20 Callithrix jacchus da colônia do DPZ (Centro de Primatas da Alemanha), foram utilizados para sua caracterização morfométrica macro e microscópica, onde os parâmetro...

  8. Disposição de efluentes da suinocultura em solo : estudo de caso

    OpenAIRE

    Bruno Marchi

    2010-01-01

    Resumo: O presente trabalho discorre sobre o estudo de caso realizado em propriedade suinícola, situada no município de Descalvado – S.P., onde, desenvolveu-se em várias frentes, compreendendo-se em um estudo qualitativo dos efluentes provindos da produção zootécnica de suínos através de analises físico-químicas. Posteriormente compararam-se os parâmetros qualitativos dos efluentes de suinocultura com os parâmetros exigidos pelo Artigo 18 do decreto nº8. 468, de 8 de Setembro de 1976 do Estad...

  9. Estudo in vitro do metabolismo microssomal hepático de agentes tripanossomicidas

    OpenAIRE

    Jean Francisco Rosa Ribeiro

    2013-01-01

    Em face das recentes exigências das agências regulatórias quanto à aprovação de novos fármacos, os estudos de biotransformação têm-se tornado uma etapa indispensável para a identificação e otimização de compostos bioativos. O objetivo desses estudos é identificar, já nas fases iniciais da descoberta de fármacos, candidatos que apresentam propriedades indesejáveis como a (i) presença de metabólitos ativos ou tóxicos; (ii) inibição de enzimas metabolizadoras; (iii) depuração metabólica inadequa...

  10. A PERCEPÇÃO DE VALOR DOS PROGRAMAS DE MILHAGENS: UM ESTUDO EXPLORATÓRIO

    Directory of Open Access Journals (Sweden)

    Lunie Imamura de Lima

    2015-07-01

    Full Text Available Este estudo propôs a avaliação da percepção de valor de programas de milhagens atribuída por participantes destes programas da cidade de Curitiba – PR. A pesquisa partiu do seguinte problema de pesquisa: “Quais são os aspectos relevantes à avaliação dos participantes de programas de milhagens para compor o valor percebido destes?” Identificaram-se dois perfis de participantes de programas de milhagens, caracterizados como “Assíduos” e “Emergentes”. Uma série de aspectos percebidos como dificuldades foram identificados bem como aspectos percebidos como benefícios. Este estudo busca contribuir com o tema de programas de lealdade, em especial os programas de milhagens propondo quatro hipóteses explicativas.

  11. Auto cuidado no tratamento pelo método de Ilizarov: um estudo de caso

    Directory of Open Access Journals (Sweden)

    Marisa Toshiko Ono Tashiro

    1995-03-01

    Full Text Available As autoras realizaram um estudo de caso com aplicação do modelo de auto cuidado de Dorothea Orem, no tratamento pelo método de Ilizarov, utilizando cuidados e orientações específicas para a recuperação e prevenção de complicações. A operacionalização do estudo constou de procedimentos relativos às demandasdecuida dos universais e terapêuticos, durante a hospitalização. As demandas foram detectadas no pré-operatório e os cuidados desenvolvidos no pós-operatório, facilitando ao paciente o aprendizado e a realização dos procedimentos necessários a dar continuidade dos mesmos no seu domicílio.

  12. Estudo da cisteína com espectroscopia de massa por tempo de voo

    OpenAIRE

    Araújo, Oscar Cardoso

    2015-01-01

    A cisteína (C3H7NO2S) é um dos aminoácidos que compõem as proteínas dos seres vivos e junto com a metionina são os únicos que tem enxofre em sua composição. Devido a grande importância dos aminoácidos, as suas propriedades físico-químicas se tornaram objetos de estudos, e são de interesse da bioquímica, da astroquímica, da astrofísica, biotecnologia, entre outras áreas. Neste trabalho fizemos o estudo da fragmentação da cisteína na região de valência. Utilizamos a técnica de Espectroscopia de...

  13. Psicossomática um estudo histórico e epistemológico

    Directory of Open Access Journals (Sweden)

    Ednéia Albino Nunes Cerchiari

    Full Text Available Artigo originado da Dissertação de Mestrado “ Uma Contribuição ao Estudo da Relação Câncer de Mama e Alexitimia”1. Trata-se de um detalhado estudo teórico sobre o tema, abrangendo a psicossomática desde suas origens às abordagens atuais. Procura fazer um levantamento de quais doenças que se encontram sob esta denominação e qual o fator etiológico predominante se biológico ou psíquico. Analisa também a alexitimia em sua relação com a psicossomática. Sugerir ainda o termo somatopsicose como o mais indicado para referenciar as doenças designadas psicossomáticas.

  14. Psicossomática um estudo histórico e epistemológico

    Directory of Open Access Journals (Sweden)

    Ednéia Albino Nunes Cerchiari

    Full Text Available Artigo originado da Dissertação de Mestrado “ Uma Contribuição ao Estudo da Relação Câncer de Mama e Alexitimia”¹. Trata-se de um detalhado estudo teórico sobre o tema, abrangendo a psicossomática desde suas origens às abordagens atuais. Procura fazer um levantamento de quais doenças que se encontram sob esta denominação e qual o fator etiológico predominante se biológico ou psíquico. Analisa também a alexitimia em sua relação com a psicossomática. Sugerir ainda o termo somatopsicose como o mais indicado para referenciar as doenças designadas psicossomáticas.

  15. O RISCO COMO ELEMENTO PARA O ESTUDO DO COMPORTAMENTO HUMANO NAS ORGANIZAÇÕES

    Directory of Open Access Journals (Sweden)

    André Luis Silva

    2011-12-01

    Full Text Available As organizações contemporaneas se caracterizam pelos processos de transformação que fazem surgir maneiras alternativas de interpretar e agir nos espaços organizacionais. Então, o que, essencialmente, têm se buscado compreender por meio do estudo do comportamento organizacional? Este estudo, de caráter bibliográfico, argumenta que a constante tensão entre organizações e as transformações do ambiente, que, por sua vez, impactam as pessoas envolvidas na situação, está norteada pelo elemento risco. Assim, o que estudamos em comportamento organizacional é a impermanência da realidade que torna o risco um valor substancial para compreender o comportamento humano quando no trato das incertezas em contextos organizacionais.

  16. O comportamento de compra compulsiva: um estudo com métodos neurocientíficos.

    OpenAIRE

    Magalhães, Maria dos Remédios Antunes

    2013-01-01

    O objetivo deste estudo foi analisar a associação entre a tomada de decisão e as reações emocionais em compradores compulsivos (CCs) e em compradores não compulsivos (CNCs). Até o momento, são desconhecidos estudos que comparem CCs a um grupo de CNCs, em relação ao impulso de comprar, à avaliação da tomada de decisão e à associação entre as reações emocionais envolvidas. A amostra foi composta por 35 consumidores, divididos entre 25 compradores compulsivos (CCs) e 10 compradores não compulsiv...

  17. Interfaces entre a Pedagogia do Teatro e os Estudos da Performance

    Directory of Open Access Journals (Sweden)

    Luciana Hartmann

    2014-11-01

    Full Text Available http://dx.doi.org/10.5902/1984644413816Este texto propõe o debate sobre pontos de contato entre a nova terminologia utilizada nos processos de ensino-aprendizagem em teatro, a Pedagogia do Teatro, e os Estudos da Performance. Partindo de uma análise dos Parâmetros Curriculares Nacionais (PCN Arte I e II e das perspectivas histórico-críticas trazidas por alguns dos principais pesquisadores brasileiros que tem se dedicado ao tema, busca-se, ao longo do texto, refletir sobre as contribuições que os Estudos da Performance podem trazer para o desenvolvimento do campo da Pedagogia do Teatro.

  18. A resposta broncodilatadora em doentes com DPOC (dados do estudo UPLIFT

    Directory of Open Access Journals (Sweden)

    D. Tashkin

    2008-07-01

    Full Text Available Resumo: No início do estudo, foi realizada espirometria antes e depois de 80 μg de ipratrópio e 400 μg de salbutamol, administrados 90 e 30 minutos antes, respectivamente. A administração de dois broncodilatadores e a sua temporalidade teve como objectivo atingir a broncodilatação máxima ou quase máxima. O FEV1 basal após esta broncodilatação, no estudo UPLIFT, permitiu: - examinar a resposta broncodilatadora aguda numa grande coorte de doentes com DPOC moderada a muito grave; - determinar o que pode ser considerado critério de resposta usando vários critérios de reversibilidade; - avaliar as características dos doentes com e sem reversibilidade.

  19. Asma e poluentes ambientais: um estudo de séries temporais

    OpenAIRE

    Amâncio,Camila Trolez; Nascimento,Luiz Fernando Costa

    2012-01-01

    OBJETIVO: O objetivo deste estudo foi estimar o risco para internação por asma em crianças após exposição a poluentes do ar em uma cidade de porte médio do Sudeste do Brasil. MÉTODOS: Foi realizado um estudo ecológico de séries temporais com dados de internação por asma em crianças com até 10 anos de idade residentes em São José dos Campos, SP, e concentrações de material particulado com diâmetro aerodinâmico inferior a 10 micra, dióxido de enxofre e ozônio; foram obtidos dados sobre umidade ...

  20. Estudo da bioatividade e compostos fitoqímicos de plantas medicinais africanas: ochlospermum angolensis

    OpenAIRE

    José, Herculano Hossi

    2017-01-01

    A Cochlospermum angolensis (Borututu) é uma planta com propriedades medicinais utilizada para o tratamento de patologia hepática e na profilaxia contra a malária. No entanto, são necessários mais estudos para comprovar a sua composição química e a sua atividade biológica. Como a utilização desta planta é recorrente no seio da população angolana, o objetivo deste estudo foi descrever o estado de arte para a sua utilização, através do conhecimento popular e revisão bibliográfi...