WorldWideScience

Sample records for gua1 genes involved

  1. Aproveitamento de águas pluviais e o reúso de águas cinzas em edifícios residenciais de Brasília – parte 1: reduções no consumo de água

    Directory of Open Access Journals (Sweden)

    Daniel Sant'Ana

    2013-12-01

    Full Text Available Sistemas de aproveitamento de águas pluviais(AAP e de reúso de águas cinzas (RAC sãoestratégias de gestão descentralizadas capazesde promover reduções na demanda urbanade água. No entanto, pouco se sabe sobre suaviabilidade para uma implementação estratégicaem edificações. Para avaliar a viabilidadedesses sistemas hidráulicos em edificações residenciais,é fundamental compreender o usodesagregado de água para estimar o potencialde redução no consumo de água e verificar aaplicabilidade, os custos e os benefícios deadaptação predial. O presente artigo foca nacaracterização dos usos-finais de água do estoquede edificações residenciais de Brasília, e naidentificação do potencial de redução do consumode água promovido por diferentes sistemasAAP e RAC. Em geral, resultados demonstramum alto índice de consumo de água em chuveiros(23,0%, máquinas de lavar roupa (21,4% edescargas sanitárias (15,3%. Já os usos externosde água representam uma pequena fraçãodo consumo predial total (3,5%. Em média, resultadosindicam que as áreas de cobertura sãocapazes de fornecer águas pluviais para suprir48% da demanda em lavagem de roupas, 60%em descargas sanitárias e 100% em lavagem depisos e irrigação. Sistemas de reúso de águascinzas apresentaram maiores valores de economiade água em lavagem de roupas (2.541m³/ano e descarga sanitárias (1.815 m³/ano.Ambos sistemas AAP e RAC são capazes depromover reduções equivalente a 811 m³/ano.

  2. Aproveitamento de águas pluviais e o reúso de águas cinzas em edifícios residenciais de Brasília – parte 1: reduções no consumo de água

    OpenAIRE

    Daniel Sant'Ana; Louise Boeger; Lilian Monteiro

    2013-01-01

    Sistemas de aproveitamento de águas pluviais(AAP) e de reúso de águas cinzas (RAC) sãoestratégias de gestão descentralizadas capazesde promover reduções na demanda urbanade água. No entanto, pouco se sabe sobre suaviabilidade para uma implementação estratégicaem edificações. Para avaliar a viabilidadedesses sistemas hidráulicos em edificações residenciais,é fundamental compreender o usodesagregado de água para estimar o potencialde redução no consumo de água e verificar aaplicabilidade, os cu...

  3. Diversitas Kelelawar (Chiroptera Penghuni Gua, Studi Gua Ngerong di Kawasan Karst Tuban Jawa Timur

    Directory of Open Access Journals (Sweden)

    Tatag Bagus Putra Prakarsa

    2013-12-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui diversitas kelelawar penghuni gua di gua Ngerong. Penelitian ini merupakan penelitian Nature Snapshop Experiment (NSE. Penelitian ini dilaksanakan pada bulan November - Desember 2011 di gua Ngerong, Desa Rengel, Kecamatan Rengel, Kabupaten Tuban, Jawa Timur. Penangkapan dilakukan dengan metode tangkap langsung. Penangkapan dilakukan dengan menggunakan misnet dan handnet. Kelelawar diidentifikasi berdasarkan pengukuran morfometri dan ciri morfologi mengacu kunci identifikasi Suyanto, 2001 dan Payne et al., 2000. Seluruh data dianalisis secara deskriptif. Di gua Ngerong terdapat 9 spesies dari 4 famili atau 60% dari total spesies kelelawar penghuni gua di kawasan karst Tuban. Enam spesies anggota Subordo Microchiroptera yang merupakan insectivor dan 3 spesies anggota Subordo Megachiroptera yang merupakan frugivor dan nictivor. Keanekaragaman di gua Ngerong tergolong tinggi dengan nilai Simpson's Diversity Index sebesar 0,76. Tingginya diversitas kelelawar penghuni gua Ngerong berbanding lurus dengan panjang lorong gua Ngerong. gua Ngerong merupakan gua terpanjang di kawasan karst Tuban, dengan panjang lorong mencapai 1800m.kata kunci: Kelelawar (Chiroptera, Diversitas, Gua Ngerong, Biospeleologi, Karst

  4. WRKY transcription factors involved in PR-1 gene expression in Arabidopsis

    NARCIS (Netherlands)

    Hussain, Rana Muhammad Fraz

    2012-01-01

    Salicylic acid (SA) is involved in mediating defense against biotrophic pathogens. The current knowledge of the SA-mediated signaling pathway and its effect on the transcriptional regulation of defense responses are reviewed in this thesis. PR-1 is a marker gene for systemic acquired resistance

  5. Viabilidade de Vibrio cholerae O1 em diferentes tipos de águas em condições experimentais

    Directory of Open Access Journals (Sweden)

    Nogueira Joseli Maria da Rocha

    2002-01-01

    Full Text Available A natureza endêmica e sazonal da cólera depende da sobrevivência de Vibrio cholerae O1 em estado viável, mas não necessariamente cultivável em nichos ambientais aquáticos durante períodos interepidêmicos, sendo de suma importância o estudo da sobrevivência deste microrganismo nesses locais. Para tal, foram coletadas, semanalmente, alíquotas de água pertencentes a duas lagoas e dois rios do Estado do Rio de Janeiro. Esses volumes foram divididos em duas porções idênticas, uma das quais foi autoclavada. Uma diluição padronizada de V. cholerae O1 Inaba e de V. cholerae O1 Ogawa, foi inoculada em três alíquotas de 100ml dessas diferentes águas e mantidas em diferentes temperaturas. A sobrevivência desses microorganismos no âmbito aquático sob esses diferentes fatores foi então analisada. Os resultados demonstraram que o V. cholerae sorogrupo O1, independente do sorotipo, é capaz de se manter em água com salinidade abaixo de 0,5? e em diferentes temperaturas, por períodos suficientes para sua disseminação através de "corpos d'água", demonstrando a necessidade de monitoramento constante em áreas de possível contaminação, principalmente onde a água é utilizada para o consumo, evitando assim, a disseminação da doença para as populações próximas a esses ambientes.

  6. Screen for genes involved in radiation survival of Escherichia coli and construction of a reference database

    Energy Technology Data Exchange (ETDEWEB)

    Sargentini, Neil J., E-mail: nsargentini@atsu.edu; Gularte, Nicholas P.; Hudman, Deborah A.

    2016-11-15

    Highlights: • 3907 Keio knockout mutants of E. coli screened for UV and X-radiation sensitivity. • 76 mutants showed significantly increased radiation sensitivity. • A database of 9 screening studies listed 352 genes only once; 103 genes, 2–7 times. • 33 genes from this study are uncommon and potentially novel. • Common and uncommon genes differ in gene function profile. - Abstract: A set of 3907 single-gene knockout (Keio collection) strains of Escherichia coli K-12 was examined for strains with increased susceptibility to killing by X- or UV-radiation. After screening with a high-throughput resazurin-based assay and determining radiation survival with triplicate clonogenic assays, we identified 76 strains (and associated deleted genes) showing statistically-significant increased radiation sensitivity compared to a control strain. To determine gene novelty, we constructed a reference database comprised of genes found in nine similar studies including ours. This database contains 455 genes comprised of 103 common genes (found 2–7 times), and 352 uncommon genes (found once). Our 76 genes includes 43 common genes and 33 uncommon (potentially novel) genes, i.e., appY, atoS, betB, bglJ, clpP, cpxA, cysB, cysE, ddlA, dgkA, dppF, dusB, elfG, eutK, fadD, glnA, groL, guaB, intF, prpR, queA, rplY, seqA, sufC,yadG, yagJ, yahD, yahO, ybaK, ybfA, yfaL, yhjV, and yiaL. Of our 33 uncommon gene mutants, 4 (12%) were sensitive only to UV-radiation, 10 (30%) only to X-radiation, and 19 (58%) to both radiations. Our uncommon mutants vs. our common mutants showed more radiation specificity, i.e., 12% vs. 9% (sensitive only to UV-); 30% vs. 16% (X-) and 58% vs. 74% (both radiations). Considering just our radiation-sensitive mutants, the median UV-radiation survival (75 J m{sup −2}) for 23 uncommon mutants was 6.84E-3 compared to 1.85E-3 for 36 common mutants (P = 0.025). Similarly, the average X-radiation survival for 29 uncommon mutants was 1.08E-2, compared to 6.19E

  7. Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis

    International Nuclear Information System (INIS)

    Kibe, A.; Shimada, K.; Tagaki, Y.

    1979-01-01

    Hybrid ColE1 plasmids called ColE1-cos lambda-guaA or ColE1-cos lambda-gal can be efficiently transduced into various E.coli K-12 cells through packaging into lambda phage particles. Using these plasmids, repair of ultraviolet-light (UV) damaged ColE1 DNAs was studied in various UV sensitive E.coli K-12 mutants. The host mutations uvrA and uvrB markedly reduced host-cell reactivation of UV-irradiated ColE1-cos lambda-guaA. Pre-existing hybrid ColE1 plasmids had no effect on the frequency of lambda phage-mediated transduction of another differentially marked hybrid ColE1 DNAs. ColE1-cos lambda-guaA and ColE1-cos lambda-gal DNAs could temporarily but not stably co-exist in E.coli K-12 recA cells. The presence of ColE1-cos lambda-gal in uvrB cells promoted the repair of super-infected UV-irradiated ColE1-cos lambda-guaA about 7-fold. The same ColE1-cos lambda-gal plasmid in a uvrB recA double mutant did not have this promoting effect. These results indicate that the effect of resident hybrid ColE1 plasmids is manifested by the host recA + gene function(s) and suggest that ColE1 plasmit itself provides no recA + -like functions. (orig.) [de

  8. Repair of ultraviolet-light damaged ColE1 factor carrying Escherichia coli genes for guanine synthesis

    Energy Technology Data Exchange (ETDEWEB)

    Kibe, A; Shimada, K; Tagaki, Y [Kyushu Univ., Fukuoka (Japan). Dept. of Biochemistry

    1979-01-01

    Hybrid ColE1 plasmids called ColE1-cos lambda-guaA or ColE1-cos lambda-gal can be efficiently transduced into various E.coli K-12 cells through packaging into lambda phage particles. Using these plasmids, repair of ultraviolet-light (UV) damaged ColE1 DNAs was studied in various UV sensitive E.coli K-12 mutants. The host mutations uvrA and uvrB markedly reduced host-cell reactivation of UV-irradiated ColE1-cos lambda-guaA. Pre-existing hybrid ColE1 plasmids had no effect on the frequency of lambda phage-mediated transduction of another differentially marked hybrid ColE1 DNAs. ColE1-cos lambda-guaA and ColE1-cos lambda-gal DNAs could temporarily but not stably co-exist in E.coli K-12 recA cells. The presence of ColE1-cos lambda-gal in uvrB cells promoted the repair of super-infected UV-irradiated ColE1-cos lambda-guaA about 7-fold. The same ColE1-cos lambda-gal plasmid in a uvrB recA double mutant did not have this promoting effect. These results indicate that the effect of resident hybrid ColE1 plasmids is manifested by the host recA/sup +/ gene function(s) and suggest that ColE1 plasmit itself provides no recA/sup +/-like functions.

  9. The BTB and CNC homology 1 (BACH1) target genes are involved in the oxidative stress response and in control of the cell cycle.

    Science.gov (United States)

    Warnatz, Hans-Jörg; Schmidt, Dominic; Manke, Thomas; Piccini, Ilaria; Sultan, Marc; Borodina, Tatiana; Balzereit, Daniela; Wruck, Wasco; Soldatov, Alexey; Vingron, Martin; Lehrach, Hans; Yaspo, Marie-Laure

    2011-07-01

    The regulation of gene expression in response to environmental signals and metabolic imbalances is a key step in maintaining cellular homeostasis. BTB and CNC homology 1 (BACH1) is a heme-binding transcription factor repressing the transcription from a subset of MAF recognition elements at low intracellular heme levels. Upon heme binding, BACH1 is released from the MAF recognition elements, resulting in increased expression of antioxidant response genes. To systematically address the gene regulatory networks involving BACH1, we combined chromatin immunoprecipitation sequencing analysis of BACH1 target genes in HEK 293 cells with knockdown of BACH1 using three independent types of small interfering RNAs followed by transcriptome profiling using microarrays. The 59 BACH1 target genes identified by chromatin immunoprecipitation sequencing were found highly enriched in genes showing expression changes after BACH1 knockdown, demonstrating the impact of BACH1 repression on transcription. In addition to known and new BACH1 targets involved in heme degradation (HMOX1, FTL, FTH1, ME1, and SLC48A1) and redox regulation (GCLC, GCLM, and SLC7A11), we also discovered BACH1 target genes affecting cell cycle and apoptosis pathways (ITPR2, CALM1, SQSTM1, TFE3, EWSR1, CDK6, BCL2L11, and MAFG) as well as subcellular transport processes (CLSTN1, PSAP, MAPT, and vault RNA). The newly identified impact of BACH1 on genes involved in neurodegenerative processes and proliferation provides an interesting basis for future dissection of BACH1-mediated gene repression in neurodegeneration and virus-induced cancerogenesis.

  10. The rice YABBY1 gene is involved in the feedback regulation of gibberellin metabolism.

    Science.gov (United States)

    Dai, Mingqiu; Zhao, Yu; Ma, Qian; Hu, Yongfeng; Hedden, Peter; Zhang, Qifa; Zhou, Dao-Xiu

    2007-05-01

    Gibberellin (GA) biosynthesis is regulated by feedback control providing a mechanism for GA homeostasis in plants. However, regulatory elements involved in the feedback control are not known. In this report, we show that a rice (Oryza sativa) YABBY1 (YAB1) gene had a similar expression pattern as key rice GA biosynthetic genes GA3ox2 and GA20ox2. Overexpression of YAB1 in transgenic rice resulted in a semidwarf phenotype that could be fully rescued by applied GA. Quantification of the endogenous GA content revealed increases of GA(20) and decreases of GA(1) levels in the overexpression plants, in which the transcripts of the biosynthetic gene GA3ox2 were decreased. Cosuppression of YAB1 in transgenic plants induced expression of GA3ox2. The repression of GA3ox2 could be obtained upon treatment by dexamethasone of transgenic plants expressing a YAB1-glucocorticoid receptor fusion. Importantly, we show that YAB1 bound to a GA-responsive element within the GA3ox2 promoter. In addition, the expression of YAB1 was deregulated in GA biosynthesis and signaling mutants and could be either transiently induced by GA or repressed by a GA inhibitor. Finally, either overexpression or cosuppression of YAB1 impaired GA-mediated repression of GA3ox2. These data together suggest that YAB1 is involved in the feedback regulation of GA biosynthesis in rice.

  11. MONITORAMENTO DA QUALIDADE DA ÁGUA TRATADA POR SISTEMAS DE DESFLUORETAÇÃO DE ÁGUAS SUBTERRÂNEAS

    Directory of Open Access Journals (Sweden)

    Gisele Steil Rodrigues

    2015-12-01

    Full Text Available O principal objetivo deste estudo foi avaliar a eficiência de sistemas de desfluoretação de águas para abastecimento público, com o propósito de contribuir para a redução da ocorrência de fluorose dental. Assim, foram construídos e instalados cinco filtros, com seis quilogramas de carvão ativado de osso, os quais foram monitorados durante dez meses. Os resultados indicaram que os filtros se mantiveram eficientes durante sete meses, tratando 4.715 ± 881 litros de água (n=5, mantendo a concentração de íons fluoretos em níveis inferiores a 1,5 mgL-1 conforme do especificado pela Portaria nº 2914, Ministério da Saúde (a concentração original da água bruta era da ordem de 3,2 mgL-1. Estes resultados demostram, assim, o potencial deste sistema para atender as necessidades de abastecimento coletivo, como em escolas, universidades e estabelecimentos comerciais, por exemplo.

  12. PLANO DE SEGURANÇA DA ÁGUA EM MANANCIAIS DE ABASTECIMENTO DE ÁGUA PARA CONSUMO HUMANO

    Directory of Open Access Journals (Sweden)

    José Manuel Pereira Vieira

    2013-03-01

    Full Text Available O progresso no acesso a água potável a nível mundial tem-se traduzido num incremento de infraestruturas que necessitam de ser adequadamente geridas por forma a garantir água segura às populações. Embora a qualidade da água para consumo humano seja um factor fundamental para a protecção da saúde pública, a sua garantia revela-se de uma grande dificuldade por razões técnicas e económicas. A implementação de planos de segurança da água, estabelecendo um processo sistemático para a identificação de perigos e procedimentos de gestão efectivos para o seu controlo em sistemas de abastecimento de água, constitui uma nova abordagem a esta problemática. No presente trabalho, apresenta-se uma proposta metodológica para a implementação de planos de segurança da água em mananciais de abastecimento, onde se estabelecem os aspectos mais relevantes a considerar, nomeadamente as limitações e necessidades relacionadas com modelos institucionais e organizacionais.

  13. The endogenous retroviral locus ERVWE1 is a bona fide gene involved in hominoid placental physiology

    Science.gov (United States)

    Mallet, François; Bouton, Olivier; Prudhomme, Sarah; Cheynet, Valérie; Oriol, Guy; Bonnaud, Bertrand; Lucotte, Gérard; Duret, Laurent; Mandrand, Bernard

    2004-01-01

    The definitive demonstration of a role for a recently acquired gene is a difficult task, requiring exhaustive genetic investigations and functional analysis. The situation is indeed much more complicated when facing multicopy gene families, because most or portions of the gene are conserved among the hundred copies of the family. This is the case for the ERVWE1 locus of the human endogenous retrovirus W family (HERV-W), which encodes an envelope glycoprotein (syncytin) likely involved in trophoblast differentiation. Here we describe, in 155 individuals, the positional conservation of this locus and the preservation of the envelope ORF. Sequencing of the critical elements of the ERVWE1 provirus showed a striking conservation among the 48 alleles of 24 individuals, including the LTR elements involved in the transcriptional machinery, the splice sites involved in the maturation of subgenomic Env mRNA, and the Env ORF. The functionality and tissue specificity of the 5′ LTR were demonstrated, as well as the fusogenic activity of the envelope polymorphic variants. Such functions were also shown to be preserved in the orthologous loci isolated from chimpanzee, gorilla, orangutan, and gibbon. This functional preservation among humans and during evolution strongly argued for the involvement of this recently acquired retroviral envelope glycoprotein in hominoid placental physiology. PMID:14757826

  14. Aging-dependent DNA hypermethylation and gene expression of GSTM1 involved in T cell differentiation.

    Science.gov (United States)

    Yeh, Shu-Hui; Liu, Cheng-Ling; Chang, Ren-Chieh; Wu, Chih-Chiang; Lin, Chia-Hsueh; Yang, Kuender D

    2017-07-25

    This study investigated whether aging was associated with epigenetic changes of DNA hypermethylation on immune gene expression and lymphocyte differentiation. We screened CG sites of methylation in blood leukocytes from different age populations, picked up genes with age-related increase of CG methylation content more than 15%, and validated immune related genes with CG hypermethylation involved in lymphocyte differentiation in the aged population. We found that 12 genes (EXHX1、 IL-10、 TSP50、 GSTM1、SLC5A5、SPI1、F2R、LMO2、PTPN6、FGFR2、MMP9、MET) were associated with promoter or exon one DNA hypermethylation in the aged group. Two immune related genes, GSTM1 and LMO2, were chosen to validate its aging-related CG hypermethylation in different leukocytes. We are the first to validate that GSTM1_P266 and LMO2_E128 CG methylation contents in T lymphocytes but not polymorphonuclear cells (PMNs) or mononuclear cells (MNCs) were significantly increased in the aged population. The GSTM1 mRNA expression in T lymphocytes but not PMNs or MNCs was inversely associated with the GSTM1 CG hypermethylation levels in the aged population studied. Further studies showed that lower GSTM1 CG methylation content led to the higher GSTM1 mRNA expression in T cells and knockdown of GSTM1 mRNA expression decreased type 1 T helper cell (Th1) differentiation in Jurkat T cells and normal adult CD4 T cells. The GSTM1_P266 hypermethylation in the aged population associated with lower GSTM1 mRNA expression was involved in Th1 differentiation, highlighting that modulation of aging-associated GSTM1 methylation may be able to enhance T helper cell immunity in the elders.

  15. APROVEITAMENTO IMEDIATO DA ÁGUA DE CHUVA

    Directory of Open Access Journals (Sweden)

    Cícero Onofre de Andrade Neto

    2013-03-01

    Full Text Available A água de chuva será, em breve, valorizada como a água mais segura que se obterá para consumo humano e com boa qualidade para diversos fins, necessitando, em muitos casos, apenas de tratamento simplificado para purificação. No Brasil, o uso de água de chuva ocorre há séculos, mas a captação imediata no meio urbano, visando ao aproveitamento em usos diversos, ainda é insipiente, e no meio rural somente na última década é que a construção de cisternas vem se disseminando para abastecimento difuso de água. Existe amplo conhecimento tecnológico internacional e nacional que deve ser utilizado de forma mais competente para o aproveitamento imediato da água de chuva, sobretudo para maximizar a relação benefício/custo, aumentar a segurança sanitária dos sistemas e assegurar a qualidade da água. Este trabalho procura mostrar as vantagens do aproveitamento imediato da água de chuva para diversos usos, apresenta e comenta avanços, e avalia e discute a segurança da água e a proteção sanitária do sistema de captação e armazenamento. Destaca a atenção que deve ser dispensada aos primeiros milímetros da cada chuva, pois estes têm grande influência sobre a qualidade da água captada em cisternas; já que esta quantidade é, geralmente, suficiente para lavar a atmosfera e a superfície de captação. Se esta água for descartada, o restante da água da chuva tem boa qualidade para diversos usos, e existem dispositivos simples, de baixo custo e de eficiência comprovada para o descarte automático das águas do primeiro milímetro de cada chuva. Quanto às ações de governo das últimas décadas para implantação de cisternas no meio rural no Brasil, é necessário avançar no desenvolvimento e adequação da tecnologia, e manter ou acelerar o ritmo das ações, mas sem perder as diretrizes políticas conquistadas, voltadas para a justiça social e a cidadania.

  16. Águas urbanas Urban waters

    Directory of Open Access Journals (Sweden)

    Carlos E. M. Tucci

    2008-01-01

    Full Text Available As águas urbanas geralmente incluem abastecimento de água e saneamento. Nessa perspectiva, saneamento envolve a coleta de tratamento de efluentes domésticos e industriais, não inclui drenagem urbana, gestão dos resíduos sólidos, porque ainda perdura uma visão desatualizada da gestão das águas urbanas da cidade. Águas urbanas envolvem componentes que permitem o desenvolvimento ambiental sustentável e utilizam os conceitos da gestão integrada dos recursos hídricos (GIRH, necessários para planejamento, implementação e manutenção da infra-estrutura da cidade. Nesse contexto, ficam denominados Gestão Integrada das Águas Urbanas. Neste artigo, analisam-se o desenvolvimento urbano e suas relações com as águas urbanas no Brasil. A gestão dos recursos hídricos no Brasil é realizada por bacias hidrográficas, e o domínio é federal ou estadual. Examinam-se as possibilidades de gestão da água na cidade e na bacia hidrográfica no contexto institucional brasileiro.Urban Waters systems generally include both water supply & sanitation facilities (WSS. Sanitation refers to domestic and industrial sewage collecting and treatment; it does not include urban stormwater or solid waste management systems. Urban water form components of a sustainable urban environment and the use of the integrated water resource management (IWRM concepts are needed for planning, implementation and maintenance of urban infrastructure. In urban environment, IWRM is referred to specifically as Integrated Urban Water Management (IUWM. In this paper urban development and its relations with urban waters in Brazil are assessed. Management of Water Resources in Brazil is developed by basins and the administration is Federal or from the state. This article assess the alternatives of water management in the city and the basin in the Brazilian institutional environment.

  17. Complementation of Saccharomyces cerevisiae mutations in genes involved in translation and protein folding (EFB1 and SSB1) with Candida albicans cloned genes.

    Science.gov (United States)

    Maneu, V; Roig, P; Gozalbo, D

    2000-11-01

    We have demonstrated that the expression of Candida albicans genes involved in translation and protein folding (EFB1 and SSB1) complements the phenotype of Saccharomyces cerevisiae mutants. The elongation factor 1beta (EF-1beta) is essential for growth and efb1 S. cerevisiae null mutant cells are not viable; however, viable haploid cells, carrying the disrupted chromosomal allele of the S. cerevisiae EFB1 gene and pEFB1, were isolated upon sporulation of a diploid strain which was heterozygous at the EFB1 locus and transformed with pEFB1 (a pEMBLYe23 derivative plasmid containing an 8-kb DNA fragment from the C. albicans genome which contains the EFB1 gene). This indicates that the C. albicans EFB1 gene encodes a functional EF-1beta. Expression of the SSB1 gene from C. albicans, which codes for a member of the 70-kDa heat shock protein family, in S. cerevisiae ssb1 ssb2 double mutant complements the mutant phenotype (poor growth particularly at low temperature, and sensitivity to certain protein synthesis inhibitors, such as paromomycin). This complementation indicates that C. albicans Ssbl may function as a molecular chaperone on the translating ribosomes, as described in S. cerevisiae. Northern blot analysis showed that SSB mRNA levels increased after mild cold shift (28 degrees C to 23 degrees C) and rapidly decreased after mild heat shift (from 28 degrees C to 37 degrees C, and particularly to 42 degrees C), indicating that SSB1 expression is regulated by temperature. Therefore, Ssb1 may be considered as a molecular chaperone whose pattern of expression is similar to that found in ribosomal proteins, according to its common role in translation.

  18. IGF-1 modulates gene expression of proteins involved in inflammation, cytoskeleton, and liver architecture.

    Science.gov (United States)

    Lara-Diaz, V J; Castilla-Cortazar, I; Martín-Estal, I; García-Magariño, M; Aguirre, G A; Puche, J E; de la Garza, R G; Morales, L A; Muñoz, U

    2017-05-01

    Even though the liver synthesizes most of circulating IGF-1, it lacks its receptor under physiological conditions. However, according to previous studies, a damaged liver expresses the receptor. For this reason, herein, we examine hepatic histology and expression of genes encoding proteins of the cytoskeleton, extracellular matrix, and cell-cell molecules and inflammation-related proteins. A partial IGF-1 deficiency murine model was used to investigate IGF-1's effects on liver by comparing wild-type controls, heterozygous igf1 +/- , and heterozygous mice treated with IGF-1 for 10 days. Histology, microarray for mRNA gene expression, RT-qPCR, and lipid peroxidation were assessed. Microarray analyses revealed significant underexpression of igf1 in heterozygous mice compared to control mice, restoring normal liver expression after treatment, which then normalized its circulating levels. IGF-1 receptor mRNA was overexpressed in Hz mice liver, while treated mice displayed a similar expression to that of the controls. Heterozygous mice showed overexpression of several genes encoding proteins related to inflammatory and acute-phase proteins and underexpression or overexpression of genes which coded for extracellular matrix, cytoskeleton, and cell junction components. Histology revealed an altered hepatic architecture. In addition, liver oxidative damage was found increased in the heterozygous group. The mere IGF-1 partial deficiency is associated with relevant alterations of the hepatic architecture and expression of genes involved in cytoskeleton, hepatocyte polarity, cell junctions, and extracellular matrix proteins. Moreover, it induces hepatic expression of the IGF-1 receptor and elevated acute-phase and inflammation mediators, which all resulted in liver oxidative damage.

  19. CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of "Wuzishatangju" (Citrus reticulata Blanco).

    Science.gov (United States)

    Li, Peng; Miao, Hongxia; Ma, Yuewen; Wang, Lu; Hu, Guibing; Ye, Zixing; Zhao, Jietang; Qin, Yonghua

    2015-09-09

    Plant S-phase kinase-associated protein 1 (SKP1) genes play crucial roles in plant development and differentiation. However, the role of SKP1 in citrus is unclear. Herein, we described a novel SKP1-like gene, designated as CrWSKP1, from "Wuzishatangju" (Citrus reticulata Blanco). The cDNA sequence of CrWSKP1 is 779 base pairs (bp) and contains an open reading frame (ORF) of 477 bp. The genomic sequence of the CrWSKP1 gene is 1296 bp with two exons and one intron. CrWSKP1 has high identity with SKP1-like genes from other plant species within two conserved regions. Approximately 85% of pollen tubes of self-pollinated CrWSKP1 transgenic tobaccos became twisted at four days after self-pollination. Pollen tube numbers of self-pollinated CrWSKP1 transformants entering into ovules were significantly fewer than that of the control. Seed number of self-pollinated CrWSKP1 transformants was significantly reduced. These results suggested that the CrWSKP1 is involved in the self-incompatibility (SI) reaction of "Wuzishatangju".

  20. Reúso de água na agricultura

    Directory of Open Access Journals (Sweden)

    H. M. M. Barros

    2015-12-01

    Full Text Available A fixação do homem nas regiões do planeta tem acontecido em função de disponibilidade de elementos necessários à sua sobrevivência. Nesse sentido, a água, tem sido a forma de energia essencial à vida e a manutenção dos ecossistemas. No entanto sua escassez tem se tornado um problema de ordem global de forma que os governantes têm cada vez mais se preocupados em gerenciar de forma responsável tal recurso. A demanda crescente por água tem feito do reuso planejado um tema atual e de grande importância. Neste sentido, considera-se o reuso de água como parte de uma atividade mais abrangente que é o uso racional ou eficiente da água, o qual compreende também o controle de perdas e desperdícios, e a minimização da produção de resíduos e do consumo de água. A utilização de águas residuárias para os mais diversos fins já é uma realidade em diversos países do mundo. No Brasil, dentre os principais fatores que contribuíram para o aumentasse o interesse pela irrigação com efluentes, se encontram: a escassez de água, o avanço técnico-científico, a legislação ambiental mais rigorosa e atuante, o maior controle da poluição ambiental, a diminuição dos custos de tratamento com solo devido à atuação, disposição e fornecimento de nutrientes, e matéria orgânica às plantas, reduzindo os custos com fertilizantes químicos comerciais. Com isso, é possível concluir que o reuso de água é importante instrumento de gestão racional dos recursos hídricos, no que diz respeito ao reuso de água para fins agrícolas.

  1. Teores de metais pesados em cambissolo irrigado com água residuária doméstica e água de poço

    Directory of Open Access Journals (Sweden)

    Adriana de Fátima Mendes Oliveira

    2015-04-01

    Full Text Available O aporte de nutrientes das águas residuárias doméstica estimula a prática do reuso na produção agrícola, porém a presença de metais pesados, mesmo que em baixas concentrações, pode acarretar problemas ambientais no longo prazo. O presente trabalho objetivou analisar os efeitos da aplicação da água residuária doméstica e água de poço na alteração dos teores de metais pesados em um cambissolo cultivado com pimenta malagueta. Para isso, uma área experimental de 744 m² foi montada no assentamento Milagres em Apodi-RN. Essa área é composta de estação de tratamento de esgoto e de sistema de irrigação por gotejamento. Os tratamentos utilizados foram T1 - 100% de aplicação água residuária doméstica tratada (ART e 0% de água de poço (AP, T2 - 75% de ART e 25% de AP, T3 - 50% de ART e 50% de AP, T4 - 25% de ART e 75% de AP e T5 - 0% de ART e 100% de AP. No período experimental foram realizadas análises químicas da ART e AP, enquanto do solo foram coletadas amostras nas camadas de 0 a 0,20 m, 0,20 a 0,40 m e 0,40 a 0,60 m para determinação dos valores de pH e do conteúdo de cobre, zinco, ferro e manganês. Os resultados indicaram que os teores de cobre, zinco, ferro e manganês do cambissolo não foram influenciados pelas proporções de água residuária doméstica tratada e de água de poço. Os valores de pH do solo apresentaram tendência de redução nas proporções com mais água residuária doméstica tratada em relação a água de poço.

  2. Trpac1, a pH response transcription regulator, is involved in cellulase gene expression in Trichoderma reesei.

    Science.gov (United States)

    He, Ronglin; Ma, Lijuan; Li, Chen; Jia, Wendi; Li, Demao; Zhang, Dongyuan; Chen, Shulin

    2014-12-01

    Fungi grow over a relatively wide pH range and adapt to extracellular pH through a genetic regulatory system mediated by a key component PacC, which is a pH transcription regulator. The cellulase production of the filamentous fungi Trichoderma reesei is sensitive to ambient pH. To investigate the connection between cellulase expression regulation and ambient pH, an ortholog of Aspergillus nidulans pacC, Trpac1, was identified and functionally characterized using a target gene deletion strategy. Deleting Trpac1 dramatically increased the cellulase production and the transcription levels of the major cellulase genes at neutral pH, which suggested Trpac1 is involved in the regulation of cellulase production. It was further observed that the expression levels of transcription factors xyr1 and ace2 also increased in the ΔTrpac1 mutant at neutral pH. In addition, the ΔTrpac1 mutant exhibited conidiation defects under neutral and alkaline pH. These results implied that Trpac1 in involved in growth and development process and cellulase gene expression in T. reesei. Copyright © 2014 Elsevier Inc. All rights reserved.

  3. Expression profiles of genes involved in tanshinone biosynthesis of ...

    Indian Academy of Sciences (India)

    Expression profiles of genes involved in tanshinone biosynthesis of two. Salvia miltiorrhiza genotypes with different tanshinone contents. Zhenqiao Song, Jianhua Wang and Xingfeng Li. J. Genet. 95, 433–439. Table 1. S. miltiorrhiza genes and primer pairs used for qRT-PCR. Gene. GenBank accession. Primer name.

  4. Identification of genes potentially involved in solute stress response in Sphingomonas wittichii RW1 by transposon mutant recovery

    Directory of Open Access Journals (Sweden)

    Edith eCoronado

    2014-11-01

    Full Text Available The term water stress refers to the effects of low water availability on microbial growth and physiology. Water availability has been proposed as a major constraint for the use of microorganisms in contaminated sites with the purpose of bioremediation. Sphingomonas wittichii RW1 is a bacterium capable of degrading the xenobiotic compounds dibenzofuran and dibenzo-p-dioxin, and has potential to be used for targeted bioremediation. The aim of the current work was to identify genes implicated in water stress in RW1 by means of transposon mutagenesis and mutant growth experiments. Conditions of low water potential were mimicked by adding NaCl to the growth media. Three different mutant selection or separation method were tested, which, however recovered different mutants. Recovered transposon mutants with poorer growth under salt-induced water stress carried insertions in genes involved in proline and glutamate biosynthesis, and further in a gene putatively involved in aromatic compound catabolism. Transposon mutants growing poorer on medium with lowered water potential also included ones that had insertions in genes involved in more general functions such as transcriptional regulation, elongation factor, cell division protein, RNA polymerase β or an aconitase.

  5. Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes.

    Science.gov (United States)

    Gomes, Eriston V; Ulhoa, Cirano J; Cardoza, Rosa E; Silva, Roberto N; Gutiérrez, Santiago

    2017-01-01

    Several Trichoderma spp. are well known for their ability to: (i) act as important biocontrol agents against phytopathogenic fungi; (ii) function as biofertilizers; (iii) increase the tolerance of plants to biotic and abiotic stresses; and (iv) induce plant defense responses via the production and secretion of elicitor molecules. In this study, we analyzed the gene-regulation effects of Trichoderma harzianum Epl-1 protein during the interactions of mutant Δ epl-1 or wild-type T. harzianum strains with: (a) the phytopathogen Botrytis cinerea and (b) with tomato plants, on short (24 h hydroponic cultures) and long periods (4-weeks old plants) after Trichoderma inoculation. Our results indicate that T. harzianum Epl-1 protein affects the in vitro expression of B. cinerea virulence genes, especially those involved in the botrydial biosynthesis ( BcBOT genes), during the mycoparasitism interaction. The tomato defense-related genes were also affected, indicating that Epl-1 is involved in the elicitation of the salicylic acid pathway. Moreover, Epl-1 also regulates the priming effect in host tomato plants and contributes to enhance the interaction with the host tomato plant during the early stage of root colonization.

  6. Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes

    Directory of Open Access Journals (Sweden)

    Eriston V. Gomes

    2017-05-01

    Full Text Available Several Trichoderma spp. are well known for their ability to: (i act as important biocontrol agents against phytopathogenic fungi; (ii function as biofertilizers; (iii increase the tolerance of plants to biotic and abiotic stresses; and (iv induce plant defense responses via the production and secretion of elicitor molecules. In this study, we analyzed the gene-regulation effects of Trichoderma harzianum Epl-1 protein during the interactions of mutant Δepl-1 or wild-type T. harzianum strains with: (a the phytopathogen Botrytis cinerea and (b with tomato plants, on short (24 h hydroponic cultures and long periods (4-weeks old plants after Trichoderma inoculation. Our results indicate that T. harzianum Epl-1 protein affects the in vitro expression of B. cinerea virulence genes, especially those involved in the botrydial biosynthesis (BcBOT genes, during the mycoparasitism interaction. The tomato defense-related genes were also affected, indicating that Epl-1 is involved in the elicitation of the salicylic acid pathway. Moreover, Epl-1 also regulates the priming effect in host tomato plants and contributes to enhance the interaction with the host tomato plant during the early stage of root colonization.

  7. Larvicultura de trairão (Hoplias lacerdae em água doce e água salinizada

    Directory of Open Access Journals (Sweden)

    Ronald Kennedy Luz

    2002-04-01

    Full Text Available O experimento foi conduzido com o objetivo de desenvolver técnicas de manejo durante a larvicultura de trairão, realizando cultivos em água doce ou ligeiramente salinizada, utilizando náuplios de Artemia como alimento vivo. No início da alimentação exógena, oito dias após a eclosão, as larvas foram contadas e estocadas em 12 recipientes com volume útil de 1,5 L cada, dotados de sistema de aeração, numa densidade de 10 larvas/L, distribuídos dentro de três tanques de 130 L, contendo água com temperatura controlada (29,5ºC. Os tanques foram totalmente cobertos com lona plástica preta, mantendo o ambiente interno escuro, e descobertos somente para os manejos diários. As larvas foram submetidas a três tratamentos: cultivo em água doce; em água a 2‰ de salinidade; e em água a 4‰ de salinidade. Cada tratamento teve quatro repetições. A alimentação foi fornecida nas proporções diárias de 300 náuplios de Artemia/larva, do primeiro ao quinto dia, de 600 náuplios de Artemia/larva, do sexto ao décimo dia e de 900 náuplios de Artemia/larva, do décimo primeiro ao décimo quinto dia, divididos em três refeições. Ao final do experimento, foram avaliadas a sobrevivência, o crescimento (comprimento e peso, a taxa de crescimento específico e a taxa de resistência ao estresse. Após 15 dias de tratamento, não foram verificadas diferenças significativas entre os resultados das variáveis analisadas. As condições de cultivo mostraram-se eficientes, possibilitando altas taxas de sobrevivência (valores médios superiores a 91,6%, sendo a Artemia um alimento atrativo. Os níveis de salinidade utilizados não afetaram o desenvolvimento das larvas e alevinos.

  8. Pré-resfriamento em água de lichia 'B3' mantida em armazenamento refrigerado

    OpenAIRE

    del Aguila,Juan Saavedra; Hofman,Peter; Campbell,Terrence; Marques,José Roberto; Heiffig-del Aguila,Lília Sichmann; Kluge,Ricardo Alfredo

    2009-01-01

    Este experimento visou a estudar o efeito de diferentes temperaturas e tempos de pré-resfriamento em água sobre a qualidade de lichias 'B3', após a frigoconservação. Os tratamentos foram: T1 = controle (sem pré-resfriamento); T2 = imersão por cinco minutos em água a 2,5°C; T3 = imersão por 10 minutos em água a 2,5°C; T4 = imersão por 20 minutos em água a 2,5°C; T5 = imersão por sete minutos em água a 6°C; T6 = imersão por 20 minutos em água a 8°C e T7 = imersão por 10 minu...

  9. Caracterização, tratamento e reúso de águas cinzas e aproveitamento de águas pluviais em edificações.

    OpenAIRE

    Simone May

    2009-01-01

    não potáveis vêm ao encontro das premissas de sustentabilidade e ao conceito de conservação de água. Sistemas de reúso de águas cinzas e sistemas de coleta e aproveitamento de águas pluviais devem seguir quatro critérios: segurança higiênica, estética, proteção ambiental e viabilidade técnica e econômica. As águas cinzas e as águas pluviais devidamente tratadas podem ser utilizadas no consumo não-potável em edificações como em bacias sanitárias, em torneiras de jardins, na irrigação de gramad...

  10. Expression profiling identifies genes involved in emphysema severity

    Directory of Open Access Journals (Sweden)

    Bowman Rayleen V

    2009-09-01

    Full Text Available Abstract Chronic obstructive pulmonary disease (COPD is a major public health problem. The aim of this study was to identify genes involved in emphysema severity in COPD patients. Gene expression profiling was performed on total RNA extracted from non-tumor lung tissue from 30 smokers with emphysema. Class comparison analysis based on gas transfer measurement was performed to identify differentially expressed genes. Genes were then selected for technical validation by quantitative reverse transcriptase-PCR (qRT-PCR if also represented on microarray platforms used in previously published emphysema studies. Genes technically validated advanced to tests of biological replication by qRT-PCR using an independent test set of 62 lung samples. Class comparison identified 98 differentially expressed genes (p p Gene expression profiling of lung from emphysema patients identified seven candidate genes associated with emphysema severity including COL6A3, SERPINF1, ZNHIT6, NEDD4, CDKN2A, NRN1 and GSTM3.

  11. Cloning and Characterizing Genes Involved in Monoterpene Induced Mammary Tumor Regression.

    Science.gov (United States)

    1996-10-01

    AD GRANT NUMBER DAMDI7-94-J-4041 TITLE: Cloning and Characterizing Genes Involved in Monoterpene Induced Mammary Tumor Regression PRINCIPAL...October 1996 Annual (1 Sep 95 - 31 Aug 96) 4. TITLE AND SUBTITLE 5. FUNDING NUMBERS Cloning and Characterizing Genes Involved in Monoterpene Induced... Monoterpene -induced/repressed genes were identified in regressing rat mammary carcinomas treated with dietary limonene using a newly developed method

  12. PROFIL RESISTIVITAS 2D PADA GUA BAWAH TANAH DENGAN METODE GEOLISTRIK KONFIGURASI WENNER-SCHLUMBERGER (STUDI KASUS GUA DAGO PAKAR, BANDUNG

    Directory of Open Access Journals (Sweden)

    Nanang Dwi Ardi

    2015-01-01

    Full Text Available Makalah ini menampilkan sebuah pendekatan dalam penyelidikan rongga-rongga di bawah tanah. Akuisisi data resistivitas telah dilakukan di Gua Dago Pakar, Bandung. Lintasan resistivitas telah diukur dan dilanjutkan dengan pengukuran secara manual dengan menggunakan tali serta GPS untuk memperkirakan bentuk gua secara akurat. Sistem konfigurasi lintasan resistivitas menggunakan konfigurasi elektroda WennerSchlumberger. Pemrosesan dan pemodelan data menggunakan perangkat lunak Res2DInv dengan metode komputasi kuadrat terkecil. Hasil dari pemodelan inverse 2D menunjukkan bahwa bentuk tubuh gua berlokasi sekitar 10 – 12,4 m di bawah lintasan resistivitas dengan rentang nilai resistivitas 540-600 Ohm meter. Hasil ini dikorelasikan dengan data geologi dan pengukuran secara manual di dalam gua. Kata kunci:  Rongga, Resistivitas, Wenner-Schlumberger, Inversi

  13. Variable phenotypes associated with 10q23 microdeletions involving the PTEN and BMPR1A genes.

    NARCIS (Netherlands)

    Menko, F.H.; Kneepkens, C.M.; Leeuw, N. de; Peeters, E.A.; Maldergem, L. van; Kamsteeg, E.J.; Davidson, R.; Rozendaal, L.; Lasham, C.A.; Peeters-Scholte, C.M.; Jansweijer, M.C.E.; Hilhorst-Hofstee, Y.; Gille, J.J.P.; Heins, Y.M.; Nieuwint, A.W.; Sistermans, E.A.

    2008-01-01

    Infantile juvenile polyposis is a rare disease with severe gastrointestinal symptoms and a grave clinical course. Recently, 10q23 microdeletions involving the PTEN and BMPR1A genes were found in four patients with infantile juvenile polyposis. It was hypothesized that a combined and synergistic

  14. Mipu1, a novel direct target gene, is involved in hypoxia inducible factor 1-mediated cytoprotection.

    Directory of Open Access Journals (Sweden)

    Kangkai Wang

    Full Text Available Mipu1 (myocardial ischemic preconditioning up-regulated protein 1, recently identified in our lab, is a novel zinc-finger transcription factor which is up-regulated during ischemic preconditioning. However, it is not clear what transcription factor contributes to its inducible expression. In the present study, we reported that HIF-1 regulates the inducible expression of Mipu1 which is involved in the cytoprotection of HIF-1α against oxidative stress by inhibiting Bax expression. Our results showed that the inducible expression of Mipu1 was associated with the expression and activation of transcription factor HIF-1 as indicated by cobalt chloride (CoCl2 treatment, HIF-1α overexpression and knockdown assays. EMSA and luciferase reporter gene assays showed that HIF-1α bound to the hypoxia response element (HRE within Mipu1 promoter region and promoted its transcription. Moreover, our results revealed that Mipu1 inhibited the expression of Bax, an important pro-apoptosis protein associated with the intrinsic pathway of apoptosis, elevating the cytoprotection of HIF-1 against hydrogen peroxide (H2O2-mediated injury in H9C2 cells. Our findings implied that Bax may be a potential target gene of transcription factor Mipu1, and provided a novel insight for understanding the cytoprotection of HIF-1 and new clues for further elucidating the mechanisms by which Mipu1 protects cell against pathological stress.

  15. Genes involved in Beauveria bassiana infection to Galleria mellonella.

    Science.gov (United States)

    Chen, Anhui; Wang, Yulong; Shao, Ying; Zhou, Qiumei; Chen, Shanglong; Wu, Yonghua; Chen, Hongwei; Liu, Enqi

    2018-05-01

    The ascomycete fungus Beauveria bassiana is a natural pathogen of hundreds of insect species and is commercially produced as an environmentally friendly mycoinsecticide. Many genes involved in fungal insecticide infection have been identified but few have been further explored. In this study, we constructed three transcriptomes of B. bassiana at 24, 48 and 72 h post infection of insect pests (BbI) or control (BbC). There were 3148, 3613 and 4922 genes differentially expressed at 24, 48 and 72 h post BbI/BbC infection, respectively. A large number of genes and pathways involved in infection were identified. To further analyze those genes, expression patterns across different infection stages (0, 12, 24, 36, 48, 60, 72 and 84 h) were studied using quantitative RT-PCR. This analysis showed that the infection-related genes could be divided into four patterns: highly expressed throughout the whole infection process (thioredoxin 1); highly expressed during early stages of infection but lowly expressed after the insect death (adhesin protein Mad1); lowly expressed during early infection but highly expressed after insect death (cation transporter, OpS13); or lowly expressed across the entire infection process (catalase protein). The data provide novel insights into the insect-pathogen interaction and help to uncover the molecular mechanisms involved in fungal infection of insect pests.

  16. APROVEITAMENTO IMEDIATO DA ÁGUA DE CHUVA

    OpenAIRE

    Cícero Onofre de Andrade Neto

    2013-01-01

    A água de chuva será, em breve, valorizada como a água mais segura que se obterá para consumo humano e com boa qualidade para diversos fins, necessitando, em muitos casos, apenas de tratamento simplificado para purificação. No Brasil, o uso de água de chuva ocorre há séculos, mas a captação imediata no meio urbano, visando ao aproveitamento em usos diversos, ainda é insipiente, e no meio rural somente na última década é que a construção de cisternas vem se disseminando para abastecimento difu...

  17. Água: direito fundamental

    OpenAIRE

    AMIN, Aleph Hassan Costa

    2013-01-01

    A poluição e o uso desordenado dos recursos hídricos, aos poucos, estão tornando a água imprópria para o consumo humano. Além disto, tanto o crescimento demográfico quanto o econômico multiplicam os usos das águas e fazem crescer sua demanda, diante de uma oferta inelástica. A junção destes fatores leva a inferir que este recurso não pode mais ser entendido como um bem infinito, pois a confrontação de sua disponibilidade com suas demandas tende a acarretar sua escassez. Neste contexto, perceb...

  18. Relatório final do ensaio de proficiência de água - 4ª rodada : medição de ânions em água mineral

    OpenAIRE

    Inmetro, Instituto Nacional de Metrologia, Qualidade e Tecnologia; Santos, Damares da Silva; Santos, Paulo Roberto da Fonseca; Sena, Rodrigo Caciano de; Cunha, Valnei Smarçaro da; Andrade, Joyce Costa; Silva, Lilian da; Almeida, Marcelo Dominguez de; Silva, Renata Souza e

    2011-01-01

    O Brasil é um país rico em águas subterrâneas, inclusive em regiões afetadas pela seca. É o sétimo produtor mundial de água mineral, com cerca de 10 bilhões de litros em 2007. Dados de 1995 mostraram 319 concessões de lavras existentes para exploração de fontes de água mineral em todo o território, obtidas junto ao Departamento Nacional de Produção Mineral do Ministério das Minas e Energia, órgão responsável pela regulamentação [1] e que em 2006, elevou-se para 814 [2]. Segundo a Agência Naci...

  19. The PIN1 family gene PvPIN1 is involved in auxin-dependent root emergence and tillering in switchgrass

    Directory of Open Access Journals (Sweden)

    Kaijie Xu

    2016-03-01

    Full Text Available Abstract Switchgrass (Panicum virgatum L.; family Poaceae is a warm-season C4 perennial grass. Tillering plays an important role in determining the morphology of aboveground parts and the final biomass yield of switchgrass. Auxin distribution in plants can affect a variety of important growth and developmental processes, including the regulation of shoot and root branching, plant resistance and biological yield. Auxin transport and gradients in plants are mediated by influx and efflux carriers. PvPIN1, a switchgrass PIN1-like gene that is involved in regulating polar transport, is a putative auxin efflux carrier. Neighbor-joining analysis using sequences deposited in NCBI databases showed that the PvPIN1gene belongs to the PIN1 family and is evolutionarily closer to the Oryza sativa japonica group. Tiller emergence and development was significantly promoted in plants subjected toPvPIN1 RNA interference (RNAi, which yielded a phenotype similar to that of wild-type plants treated with the auxin transport inhibitor TIBA (2,3,5-triiodobenzoic acid. A transgenic approach that inducedPvPIN1 gene overexpression or suppression altered tiller number and the shoot/root ratio. These data suggest that PvPIN1plays an important role in auxin-dependent adventitious root emergence and tillering.

  20. Desenvolvimento de mudas de pinhão-manso irrigadas com água salina

    Directory of Open Access Journals (Sweden)

    Fábio Santos Matos

    2013-08-01

    Full Text Available O uso de água salina na irrigação torna-se importante alternativa diante daescassez de água de boa qualidade em todo o mundo. O pinhão-manso (Jatropha curcas L. possui baixa exigência hídrica, sobrevive e apresenta produção satisfatória em solos de baixa fertilidade. No entanto, a sua produção é maior em cultivos irrigados, o que reforça a necessidade de desenvolvimento de pesquisas para uso de água salina. Este trabalho objetivou avaliar o efeito de diferentes níveis de salinidade da água de irrigação nas características morfofisiológicas de mudas de pinhão-manso. Para isso, foi conduzido experimento em casa de vegetação com interceptação de 50 % da radiação solar, localizada na Universidade Estadual de Goiás, Ipameri, Goiás. O experimento foi conduzido em vasos com capacidade de 4 L de solo, utilizando-se o delineamento inteiramente casualizado, com quatro tratamentos e cinco repetições. As plantas foram irrigadas diariamente com 150 mL de água não salina, durante os 30 primeiros dias após a germinação das sementes. Do 31º ao 50º dia, as plantas foram submetidas a quatro tratamentos: plantas diariamente irrigadas com água de condutividade elétrica igual a 0,5; 8; 16 e 24 dS m-1. Aos 50 dias após a germinação, analisaram-se as seguintes características nas mudas de pinhão-manso: número de folhas; altura de planta; diâmetro de ramo; teor relativo de água; área foliar; clorofila total; razões de massa radicular, massa caulinar, massa foliar e parte aérea/sistema radicular; e biomassa total. Os resultados evidenciaram que as mudas de pinhão-manso irrigadas com água de condutividade elétrica 8 dS m-1 não apresentaram redução do crescimento vegetativo. Todavia, a água de irrigação com condutividade elétrica 16 dS m-1 causou redução no crescimento vegetativo e elevou a senescência e abscisão foliar. Água com condutividade elétrica elétrica de pinhão-manso na fase de mudas.

  1. ESKIMO1 is a key gene involved in water economy as well as cold acclimation and salt tolerance

    Directory of Open Access Journals (Sweden)

    Yu Agnes

    2008-12-01

    Full Text Available Abstract Background Drought is a major social and economic problem resulting in huge yield reduction in the field. Today's challenge is to develop plants with reduced water requirements and stable yields in fluctuating environmental conditions. Arabidopsis thaliana is an excellent model for identifying potential targets for plant breeding. Drought tolerance in the field was successfully conferred to crops by transferring genes from this model species. While involved in a plant genomics programme, which aims to identify new genes responsible for plant response to abiotic stress, we identified ESKIMO1 as a key gene involved in plant water economy as well as cold acclimation and salt tolerance. Results All esk1 mutants were more tolerant to freezing, after acclimation, than their wild type counterpart. esk1 mutants also showed increased tolerance to mild water deficit for all traits measured. The mutant's improved tolerance to reduced water supply may be explained by its lower transpiration rate and better water use efficiency (WUE, which was assessed by carbon isotope discrimination and gas exchange measurements. esk1 alleles were also shown to be more tolerant to salt stress. Transcriptomic analysis of one mutant line and its wild-type background was carried out. Under control watering conditions a number of genes were differentially expressed between the mutant and the wild type whereas under mild drought stress this list of genes was reduced. Among the genes that were differentially expressed between the wild type and mutant, two functional categories related to the response to stress or biotic and abiotic stimulus were over-represented. Under salt stress conditions, all gene functional categories were represented equally in both the mutant and wild type. Based on this transcriptome analysis we hypothesise that in control conditions the esk1 mutant behaves as if it was exposed to drought stress. Conclusion Overall our findings suggest that the

  2. O contexto global e nacional frente aos desafios do acesso adequado à água para consumo humano The global and national context regarding the challenges involved in ensuring adequate access to water for human consumption

    Directory of Open Access Journals (Sweden)

    Lia Giraldo da Silva Augusto

    2012-06-01

    Full Text Available Este artigo tem como objetivo analisar os desafios do acesso à água para consumo humano, considerando-se o contexto internacional e nacional. A partir da deliberação da ONU de que o acesso à água limpa e segura é um direito fundamental de todo ser humano são identificadas vulnerabilidades que podem se constituir em restrições ao acesso. A distribuição da água e das populações no planeta, a poluição, as políticas e gestões inadequadas produzem injustiça ambiental. A iniquidade de acesso a água constitui-se na crise contemporânea da água. A partir da década de 1980, emerge o mercado transnacional de água pelo controle privado que ocorre em três níveis principais: de mananciais superficiais e subterrâneos; de água engarrafada; e dos serviços públicos de abastecimento. Os conflitos dos usos múltiplos dos recursos hídricos, do mercado e dos problemas ambientais têm contribuído para a vulnerabilização da saúde das populações e dos ecossistemas. São necessárias políticas públicas adequadas ao exercício do direito humano fundamental de acesso a água com qualidade.The scope of this article is to analyze the challenges involved in ensuring access to water for human consumption taking the international and national context into consideration. Based on the UN declaration that access to safe and clean drinking water is a fundamental human right, vulnerabilities are identified that can consist in restrictions to access to adequate supplies. The distribution of water and the population across the planet, pollution, inadequate policies and management lead to environmental injustice. The iniquity of access to water constitutes the contemporary water crisis. From the 1980s onwards, the transnational water market emerged for private control that occurs at three main levels: surface and underground water sources; bottled water; and public water supply services. The conflicts of the multiple uses of water resources, the

  3. Água levemente salinizada aumenta a eficiência da larvicultura de peixes neotropicais

    Directory of Open Access Journals (Sweden)

    Rosângela Kiyoko Jomori

    2013-08-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito de água levemente salinizada sobre a larvicultura intensiva do tambaqui, matrinxã, apaiari e piau, durante os dias iniciais de alimentação. As larvas foram mantidas em água artificialmente salinizada, em concentrações de 0 (água doce a 14 g L-1 de NaCl (intervalo de 2,0 g L-1, e foram alimentadas com duas porções diárias de náuplios de Artemia, de acordo com protocolo para cada espécie, em delineamento inteiramente casualizado, com três repetições. Larvas de tambaqui, matrinxã e apaiari podem ser cultivadas em até 2 g L-1 de concentração salina, sem prejuízos ao crescimento e à sobrevivência. Larvas de piau foram mais tolerantes e suportaram até 4 g L-1 de concentração salina. Acima disto, a mortalidade dos peixes aumentou e chegou a 100% à concentração de 6 g L-1 (matrinxã e apaiari e de 10 g L-1 (tambaqui. A salinização da água a 2 g L-1 proporcionou maior taxa de sobrevivência a larvas de matrinxã e maior crescimento a larvas de tambaqui, apaiari e piau. Estas duas últimas espécies apresentaram melhor crescimento com a maior quantidade de náuplios. Água salinizada a 2 g L-1 é benéfica para as espécies estudadas, pois otimiza o potencial de crescimento das larvas e o uso de náuplios de Artemia como alimento vivo.

  4. An ethylene-responsive enhancer element is involved in the senescence-related expression of the carnation glutathione-S-transferase (GST1) gene.

    OpenAIRE

    Itzhaki, H; Maxson, J M; Woodson, W R

    1994-01-01

    The increased production of ethylene during carnation petal senescence regulates the transcription of the GST1 gene encoding a subunit of glutathione-S-transferase. We have investigated the molecular basis for this ethylene-responsive transcription by examining the cis elements and trans-acting factors involved in the expression of the GST1 gene. Transient expression assays following delivery of GST1 5' flanking DNA fused to a beta-glucuronidase receptor gene were used to functionally define ...

  5. Potencial de economia de água em duas escolas em Florianópolis, SC

    Directory of Open Access Journals (Sweden)

    Gabriel Balparda Fasola

    Full Text Available O objetivo deste artigo é apresentar os usos finais de água potável stimados e o potencial de economia de água potável obtido por meio e um sistema de aproveitamento de água pluvial, reúso de águas inzas, equipamentos economizadores, ou combinação deles, em duas nicípio de Florianópolis, Santa Catarina. Para isso, foi necessário obter informações sobre os hábitos de consumo dos ocupantes e realizar medições de vazão e levantamento de dados e aparelhos sanitários das escolas (uma estadual e outra municipal. Para o cálculo do potencial de economia obtido através do aproveitamento de água pluvial utilizou-se o programa Netuno 2.1. No reúso de águas cinzas, consideraram-se como oferta de água para a escola municipal os efluentes provenientes das torneiras de banheiros e da máquina de lavar roupas, e para a escola estadual foram considerados apenas os efluentes provenientes das torneiras de banheiros. Por último, as reduções de consumo de água potável, decorrentes da instalação de equipamentos economizadores, foram avaliadas para bacias sanitárias, mictórios, torneiras comuns e torneiras de fechamento automático. Como resultado, obteve-se um consumo de 28,8 litros/pessoa.dia na escola municipal, e de 25,3 litros/pessoa.dia na escola estadual. Com relação aos usos finais, as torneiras da cozinha e os mictórios destacaram-se como os maiores responsáveis pelo consumo de água. No que se refere ao potencial de economia de água, o resultado mais expressivo foi de 27,8% para a escola municipal e de 72,7% para a escola estadual, combinando equipamentos economizadores e aproveitamento de água pluvial.

  6. LMI1-like genes involved in leaf margin development of Brassica napus.

    Science.gov (United States)

    Ni, Xiyuan; Liu, Han; Huang, Jixiang; Zhao, Jianyi

    2017-06-01

    In rapeseed (Brassica napus L.), leaf margins are variable and can be entire, serrate, or lobed. In our previous study, the lobed-leaf gene (LOBED-LEAF 1, BnLL1) was mapped to a 32.1 kb section of B. napus A10. Two LMI1-like genes, BnaA10g26320D and BnaA10g26330D, were considered the potential genes that controlled the lobed-leaf trait in rapeseed. In the present study, these two genes and another homologous gene (BnaC04g00850D) were transformed into Arabidopsis thaliana (L.) Heynh. plants to identify their functions. All three LMI1-like genes of B. napus produced serrate leaf margins. The expression analysis indicated that the expression level of BnaA10g26320D determined the difference between lobed- and entire-leaved lines in rapeseed. Therefore, it is likely that BnaA10g26320D corresponds to BnLL1.

  7. Identification of genes involved in DNA replication of the Autographa californica baculovirus

    NARCIS (Netherlands)

    Kool, M.; Ahrens, C. H.; Goldbach, R. W.; Rohrmann, G. F.; Vlak, J. M.

    1994-01-01

    By use of a transient replication assay, nine genes involved in DNA replication were identified in the genome of the Autographa californica baculovirus. Six genes encoding helicase, DNA polymerase, IE-1, LEF-1, LEF-2, and LEF-3 are essential for DNA replication while three genes encoding P35, IE-2,

  8. Salinidade da Água e Biofertilizante Bovino: Efeito sobre a Biometria do Pimentão

    Directory of Open Access Journals (Sweden)

    Vinícius Batista Campos

    2009-10-01

    Full Text Available Objetivou-se avaliar o efeito da salinidade da água de irrigação e biofertilizante bovino no comportamento vegetativo do pimentão, cultivar “All Big”. O experimento foi conduzido em abrigo telado, utilizando como substrato material dos primeiros 10 cm de um Latossolo Amarelo Distrófico, acondicionado em vasos de 3 L, adotando-se o delineamento inteiramente casualizado, em esquema fatorial 5x2 correspondente a condutividade elétrica da água (0,5; 1,0; 2,0; 3,0 e 4,0 dS m-1, na ausência e presença de biofertilizante bovino aplicado ao solo na forma líquida, diluído em água na proporção de 1:1. Aos 60 dias após a semeadura, a salinidade da água de irrigação influenciou negativamente a altura das plantas, número de folhas e área foliar do pimentão, contudo, em menor intensidade às plantas submetidas ao biofertilizante bovino. Apenas o diâmetro do caule não foi afetado pelos sais presentes na água de irrigação, mas essas plantas apresentaram diâmetro superior quando tratadas com o insumo.

  9. Apolipoprotein gene involved in lipid metabolism

    Science.gov (United States)

    Rubin, Edward; Pennacchio, Len A.

    2007-07-03

    Methods and materials for studying the effects of a newly identified human gene, APOAV, and the corresponding mouse gene apoAV. The sequences of the genes are given, and transgenic animals which either contain the gene or have the endogenous gene knocked out are described. In addition, single nucleotide polymorphisms (SNPs) in the gene are described and characterized. It is demonstrated that certain SNPs are associated with diseases involving lipids and triglycerides and other metabolic diseases. These SNPs may be used alone or with SNPs from other genes to study individual risk factors. Methods for intervention in lipid diseases, including the screening of drugs to treat lipid-related or diabetic diseases are also disclosed.

  10. RESPOSTA DO TOMATEIRO A DIFERENTES TENSÕES DE ÁGUA NO SOLO

    Directory of Open Access Journals (Sweden)

    Rogério Rangel Rodrigues

    2017-01-01

    Full Text Available Objetivou-se com esse trabalho avaliar a resposta do tomateiro (Solanum lycopersicon L., híbrido Dominador F1, a diferentes tensões de água no solo. O experimento foi conduzido em casa de vegetação na Universidade Federal de Lavras, Lavras, MG. Os tratamentos constituíram-se de seis tensões de água no solo (20, 45, 70, 95, 120 e 145 kPa, com quatro repetições, em um delineamento em blocos casualizados. As variáveis avaliadas foram: produção total, altura da planta, diâmetro do caule, acidez titulável e lâmina aplicada. Os resultados demonstraram que, com exceção da acidez titulável, as demais variáveis avaliadas apresentaram menores valores com o aumento da tensão de água no solo. Para a obtenção de maior produção de frutos do tomateiro Dominador F1, as irrigações devem ser realizadas quando a tensão de água no solo, a 0,20 cm de profundidade, estiver em torno de 45 kPa.

  11. Involvement of aberrant DNA methylation on reduced expression of lysophosphatidic acid receptor-1 gene in rat tumor cell lines

    International Nuclear Information System (INIS)

    Tsujiuchi, Toshifumi; Shimizu, Kyoko; Onishi, Mariko; Sugata, Eriko; Fujii, Hiromasa; Mori, Toshio; Honoki, Kanya; Fukushima, Nobuyuki

    2006-01-01

    Lysophosphatidic acid (LPA) is a bioactive phospholipid that stimulates cell proliferation, migration, and protects cells from apoptosis. It interacts with specific G protein-coupled transmembrane receptors. Recently, it has been reported that alterations of LPA receptor expression might be important in the malignant transformation of tumor cells. Therefore, to assess an involvement of DNA methylation in reduced expression of the LPA receptor-1 (lpa1) gene, we investigated the expression of the lpa1 gene and its DNA methylation patterns in rat tumor cell lines. Both rat brain-derived neuroblastoma B103 and liver-derived hepatoma RH7777 cells used in this study indicated no expression of lpa1. For the analysis of methylation status, bisulfite sequencing was performed with B103 and RH7777 cells, comparing with other lpa1 expressed cells and normal tissues of brain and liver. The lpa1 expressed cells and tissues were all unmethylated in this region of lpa1. In contrast, both B103 and RH7777 cells were highly methylated, correlating with reduced expression of the lpa1. Treatment with 5-aza 2'-deoxycytidine induced expression of lpa1 gene in B103 and RH7777 cells after 24 h. In RH7777 cells treated with 5-aza 2'-deoxycytidine, stress fiber formation was also observed in response to LPA in RH7777 cells, but not in untreated RH7777 cells. These results suggest that aberrant DNA methylation of the lpa1 gene may be involved in its reduced expression in rat tumor cells

  12. MC1R gene variants involvement in human OCA phenotype

    OpenAIRE

    Saleha Shamim; Khan Taj Ali; Zafar Shaista

    2016-01-01

    Oculocutaneous albinism (OCA) is a genetic disorder of melanin synthesis that results in hypopigmentation in hair, skin and eyes. OCA has been reported in individuals from all ethnic backgrounds but it is more common among those with Europeans ancestry. OCA is heterogeneous group of disorders and seven types of OCA are caused by mutations in TYR (OCA1), OCA2 (OCA2), TYRP1 (OCA3), SLC45A2 (OCA4), SLC24A5 (OCA6) and C10oRF11 (OCA7) genes. However, MC1R gene variants have been reported that modi...

  13. Monitoramento da qualidade da água tratada por sistema de desfluoretação de águas subterrâneas do campus da Universidade de Santa Cruz do Sul (UNISC, cidade de Santa Cruz do Sul, RS, Brasil

    Directory of Open Access Journals (Sweden)

    Cristiane Pappis

    2017-01-01

    Full Text Available O consumo da água com níveis de flúor acima do permitido pela Portaria nº 2914/2011 do Ministério da Saúde (1,5 mg L-1, é um dos principais fatores que levam à fluorose dental, principalmente em comunidades mais distantes, onde o acesso a água potável de boa qualidade é restrito. Neste contexto, este trabalho teve como objetivo avaliar a eficiência do carvão ativado de osso bovino (capacidade de adsorção de 1000 mg g-1, na adsorção de íons fluoreto de águas subterrâneas que são usadas para o abastecimento público. Amostras da água de poços artesianos localizados no campus da UNISC com uma concentração de fluoretos entre 2,3 e 3,4 mg L-1 foram coletadas e analisadas semanalmente, considerando as seguintes variáveis: condutividade elétrica, pH, turbidez, e seus fluoretos. Os resultados indicaram que os cinco filtros foram altamente eficientes para remover parcialmente o flúor, tratando em média 15.398 ± 3.487 litros de água (Coeficiente de Variação, CV=22,6%, dentro do especificado pela portaria nº 2914/2011, a qual determina que a concentração de íons fluoretos na água para o consumo humano seja menor ou igual a 1,5 mg L-1. Desta forma, provamos a eficiência do carvão ativado de osso na adsorção dos íons fluoretos da água, destacando que o sistema de desfluoretação pode ser utilizado para uso coletivo, tanto em escolas como universidades.

  14. Transcriptome analyses reveal the involvement of both C and N termini of cryptochrome 1 in its regulation of phytohormone-responsive gene expression in Arabidopsis

    Directory of Open Access Journals (Sweden)

    Wenxiu eWang

    2016-03-01

    Full Text Available Cryptochromes (CRY are blue-light photoreceptors that mediate various light responses in plants and animals. It has long been demonstrated that Arabidopsis CRY (CRY1 and CRY2 C termini (CCT1 and CCT2 mediate light signaling through direct interaction with COP1. Most recently, CRY1 N terminus (CNT1 has been found to be involved in CRY1 signaling independent of CCT1, and implicated in the inhibition of gibberellin acids (GA/brassinosteroids (BR/auxin-responsive gene expression. Here, we performed RNA-Seq assay using transgenic plants expressing CCT1 fused to β-glucuronidase (GUS-CCT1, abbreviated as CCT1, which exhibit a constitutively photomorphogenic phenotype, and compared the results with those obtained previously from cry1cry2 mutant and the transgenic plants expressing CNT1 fused to nuclear localization signal sequence (NLS-tagged YFP (CNT1-NLS-YFP, abbreviated as CNT1, which display enhanced responsiveness to blue light. We found that 2,903 (67.85% of the CRY-regulated genes are regulated by CCT1 and that 1,095 of these CCT1-regulated genes are also regulated by CNT1. After annotating the gene functions, we found that CCT1 is involved in mediating CRY1 regulation of phytohormone-responsive genes, like CNT1, and that about half of the up-regulated genes by GA/BR/auxin are down-regulated by CCT1 and CNT1, consistent with the antagonistic role for CRY1 and these phytohormones in regulating hypocotyl elongation. Physiological studies showed that both CCT1 and CNT1 are likely involved in mediating CRY1 reduction of seedlings sensitivity to GA under blue light. Furthermore, protein expression studies demonstrate that the inhibition of GA promotion of HY5 degradation by CRY1 is likely mediated by CCT1, but not by CNT1. These results give genome-wide transcriptome information concerning the signaling mechanism of CRY1, unraveling possible involvement of its C and N termini in its regulation of response of GA and likely other phytohormones.

  15. APROVEITAMENTO DE ÁGUA DE CHUVA SEM TRATAMENTO EM UMA RESIDÊNCIA.

    OpenAIRE

    Roberval Bresolin

    2010-01-01

    Água fonte da vida! Pesquisas realizadas, até o momento, sobre a vida na terra são provadas e comprovadas que não existiríamos como somos hoje se não houvesse água. Qualquer forma de vida depende da água para a sua sobrevivência e para o seu desenvolvimento; mesmo formas de vidas por mais primitivas dependem da água para sobreviver. Somente 2,8%, ou seja, um percentual muito baixo diante do universo da Água do Planeta Terra é doce e grande parte desta é imprópria para o consumo humano s...

  16. Wheat F-Box Protein Gene TaFBA1 Is Involved in Plant Tolerance to Heat Stress

    Directory of Open Access Journals (Sweden)

    Qinxue Li

    2018-04-01

    Full Text Available Adverse environmental conditions, including high temperature, often affect the growth and production of crops worldwide. F-box protein, a core component of the Skp1-Cullin-F-box (SCF E3 ligase complex, plays an important role in abiotic stress responses. A previously cloned gene from wheat, TaFBA1, encodes a homologous F-box protein. A Yeast two-Hybrid (Y2H assay showed that TaFBA1 interacted with other SCF proteins. We found that the expression of TaFBA1 could be induced by heat stress (45°C. Overexpression of TaFBA1 enhanced heat stress tolerance in transgenic tobacco, because growth inhibition was reduced and photosynthesis increased as compared with those in the wild type (WT plants. Furthermore, the accumulation of H2O2, O2-, and carbonyl protein decreased and cell damage was alleviated in transgenic plants under heat stress, which resulted in less oxidative damage. However, the transgenic plants contained more enzymatic antioxidants after heat stress, which might be related to the regulation of some antioxidant gene expressions. The qRT-PCR analysis showed that the overexpression of TaFBA1 upregulated the expression of genes involved in reactive oxygen species (ROS scavenging, proline biosynthesis, and abiotic stress responses. We identified the interaction of TaFBA1 with Triticum aestivum stress responsive protein 1 (TaASRP1 by Y2H assay and bimolecular fluorescence complementation (BiFC assay. The results suggested that TaFBA1 may improve enzymatic antioxidant levels and regulate gene expression by interacting with other proteins, such as TaASRP1, which leads to the enhanced heat stress tolerance seen in the transgenic plants.

  17. Polymorphisms of genes involved in polycyclic aromatic hydrocarbons’ biotransformation and atherosclerosis

    Science.gov (United States)

    Marinković, Natalija; Pašalić, Daria; Potočki, Slavica

    2013-01-01

    Polycyclic aromatic hydrocarbons (PAHs) are among the most prevalent environmental pollutants and result from the incomplete combustion of hydrocarbons (coal and gasoline, fossil fuel combustion, byproducts of industrial processing, natural emission, cigarette smoking, etc.). The first phase of xenobiotic biotransformation in the PAH metabolism includes activities of cytochrome P450 from the CYP1 family and microsomal epoxide hydrolase. The products of this biotransformation are reactive oxygen species that are transformed in the second phase through the formation of conjugates with glutathione, glucuronate or sulphates. PAH exposure may lead to PAH-DNA adduct formation or induce an inflammatory atherosclerotic plaque phenotype. Several genetic polymorphisms of genes encoded for enzymes involved in PAH biotransformation have been proven to lead to the development of diseases. Enzyme CYP P450 1A1, which is encoded by the CYP1A1 gene, is vital in the monooxygenation of lipofilic substrates, while GSTM1 and GSTT1 are the most abundant isophorms that conjugate and neutralize oxygen products. Some single nucleotide polymorphisms of the CYP1A1 gene as well as the deletion polymorphisms of GSTT1 and GSTM1 may alter the final specific cellular inflammatory respond. Occupational exposure or conditions from the living environment can contribute to the production of PAH metabolites with adverse effects on human health. The aim of this study was to obtain data on biotransformation and atherosclerosis, as well as data on the gene polymorphisms involved in biotransformation, in order to better study gene expression and further elucidate the interaction between genes and the environment. PMID:24266295

  18. REUTILIZAÇÃO DA SOBRA DE ÁGUA PERMEADA E DE REJEITO DE UMA CENTRAL DE TRATAMENTO DE ÁGUA POR OSMOSE REVERSA DE UMA UNIDADE DE HEMODIÁLISE HOSPITALAR

    Directory of Open Access Journals (Sweden)

    L. Ribeiro

    2016-09-01

    Full Text Available Esse trabalho trata da possibilidade de reutilizar a água do rejeito e a sobra da água permeada proveniente de um sistema de Osmose Reversa, OR, da unidade de hemodiálise do Hospital das Clínicas de Ribeirão Preto (HCRP-USP. O estudo foi feito por meio de levantamento estrutural com plantas físicas e ‘layout’ do local, fazendo visitas a esse local para analisar a qualidade e verificar a quantidade de água utilizada no mesmo e, principalmente, do estudo das características bacteriológicas e físico-químicas da água a ser reaproveitada. O resultado obtido das análises da sobra de água permeada e do rejeito mostrou que é possível reutilizar a sobra de água permeada diretamente na caixa central do HCRP-USP, pois esse procedimento não altera a qualidade da água de abastecimento do hospital, sendo somente de 0,73 % de diluição. Com a instalação do sistema de reaproveitamento da sobra de água permeada o HCRP-USP passou a ter uma economia em volume de água de 575 m3/mês. O custo total de implantação do sistema não passou de 5.809,43 reais, ou seja, com pouquíssimo recurso financeiro o hospital obteve uma grande economia no insumo água

  19. Mapping hisS, the structural gene for histidyl-transfer ribonucleic acid synthetase, in Escherichia coli.

    Science.gov (United States)

    Parker, J; Fishman, S E

    1979-04-01

    The structural gene for histidyl-tRNA synthetase was localized to 53.8 min on the Escherichia coli genome. The gene order in this region was determined to be dapE-purC-upp-purG-(guaA, guaB)-hisS-glyA.

  20. Association analysis of schizophrenia on 18 genes involved in neuronal migration

    DEFF Research Database (Denmark)

    Kähler, Anna K; Djurovic, Srdjan; Kulle, Bettina

    2008-01-01

    neuronal function, morphology, and formation of synaptic connections. We have investigated the putative association between SZ and gene variants engaged in the neuronal migration process, by performing an association study on 839 cases and 1,473 controls of Scandinavian origin. Using a gene-wide approach......Several lines of evidence support the theory of schizophrenia (SZ) being a neurodevelopmental disorder. The structural, cytoarchitectural and functional brain abnormalities reported in patients with SZ, might be due to aberrant neuronal migration, since the final position of neurons affects......, tagSNPs in 18 candidate genes have been genotyped, with gene products involved in the neuron-to-glial cell adhesion, interactions with the DISC1 protein and/or rearrangements of the cytoskeleton. Of the 289 markers tested, 19 markers located in genes MDGA1, RELN, ITGA3, DLX1, SPARCL1, and ASTN1...

  1. Cold shock protein YB-1 is involved in hypoxia-dependent gene transcription

    International Nuclear Information System (INIS)

    Rauen, Thomas; Frye, Bjoern C.; Wang, Jialin; Raffetseder, Ute; Alidousty, Christina; En-Nia, Abdelaziz; Floege, Jürgen; Mertens, Peter R.

    2016-01-01

    Hypoxia-dependent gene regulation is largely orchestrated by hypoxia-inducible factors (HIFs), which associate with defined nucleotide sequences of hypoxia-responsive elements (HREs). Comparison of the regulatory HRE within the 3′ enhancer of the human erythropoietin (EPO) gene with known binding motifs for cold shock protein Y-box (YB) protein-1 yielded strong similarities within the Y-box element and 3′ adjacent sequences. DNA binding assays confirmed YB-1 binding to both, single- and double-stranded HRE templates. Under hypoxia, we observed nuclear shuttling of YB-1 and co-immunoprecipitation assays demonstrated that YB-1 and HIF-1α physically interact with each other. Cellular YB-1 depletion using siRNA significantly induced hypoxia-dependent EPO production at both, promoter and mRNA level. Vice versa, overexpressed YB-1 significantly reduced EPO-HRE-dependent gene transcription, whereas this effect was minor under normoxia. HIF-1α overexpression induced hypoxia-dependent gene transcription through the same element and accordingly, co-expression with YB-1 reduced HIF-1α-mediated EPO induction under hypoxic conditions. Taken together, we identified YB-1 as a novel binding factor for HREs that participates in fine-tuning of the hypoxia transcriptome. - Highlights: • Hypoxia drives nuclear translocation of cold shock protein YB-1. • YB-1 physically interacts with hypoxia-inducible factor (HIF)-1α. • YB-1 binds to the hypoxia-responsive element (HRE) within the erythropoietin (EPO) 3′ enhancer. • YB-1 trans-regulates transcription of hypoxia-dependent genes such as EPO and VEGF.

  2. Mapping hisS, the structural gene for histidyl-transfer ribonucleic acid synthetase, in Escherichia coli.

    Science.gov (United States)

    Parker, J; Fishman, S E

    1979-01-01

    The structural gene for histidyl-tRNA synthetase was localized to 53.8 min on the Escherichia coli genome. The gene order in this region was determined to be dapE-purC-upp-purG-(guaA, guaB)-hisS-glyA. PMID:374370

  3. Screening key candidate genes and pathways involved in insulinoma by microarray analysis.

    Science.gov (United States)

    Zhou, Wuhua; Gong, Li; Li, Xuefeng; Wan, Yunyan; Wang, Xiangfei; Li, Huili; Jiang, Bin

    2018-06-01

    Insulinoma is a rare type tumor and its genetic features remain largely unknown. This study aimed to search for potential key genes and relevant enriched pathways of insulinoma.The gene expression data from GSE73338 were downloaded from Gene Expression Omnibus database. Differentially expressed genes (DEGs) were identified between insulinoma tissues and normal pancreas tissues, followed by pathway enrichment analysis, protein-protein interaction (PPI) network construction, and module analysis. The expressions of candidate key genes were validated by quantitative real-time polymerase chain reaction (RT-PCR) in insulinoma tissues.A total of 1632 DEGs were obtained, including 1117 upregulated genes and 514 downregulated genes. Pathway enrichment results showed that upregulated DEGs were significantly implicated in insulin secretion, and downregulated DEGs were mainly enriched in pancreatic secretion. PPI network analysis revealed 7 hub genes with degrees more than 10, including GCG (glucagon), GCGR (glucagon receptor), PLCB1 (phospholipase C, beta 1), CASR (calcium sensing receptor), F2R (coagulation factor II thrombin receptor), GRM1 (glutamate metabotropic receptor 1), and GRM5 (glutamate metabotropic receptor 5). DEGs involved in the significant modules were enriched in calcium signaling pathway, protein ubiquitination, and platelet degranulation. Quantitative RT-PCR data confirmed that the expression trends of these hub genes were similar to the results of bioinformatic analysis.The present study demonstrated that candidate DEGs and enriched pathways were the potential critical molecule events involved in the development of insulinoma, and these findings were useful for better understanding of insulinoma genesis.

  4. AVALIAÇÃO DA NECESSIDADE DO REÚSO DE ÁGUAS RESIDUAIS

    Directory of Open Access Journals (Sweden)

    Djeson Mateus Alves da Costa

    2007-12-01

    Full Text Available O objetivo desse trabalho foi, por meio uma pesquisa bibliográfica, apresentar um relato sobre a importância do racionamento do consumo de água através de seu reúso, descrever o ciclo global da água e quantificar seus principais reservatórios na terra, identificar as fontes potencialmente poluidoras das reservas hídricas, apresentar os aspectos críticos sobre a disponibilidade e o consumo de água no mundo, mencionar as tecnologias aplicadas no tratamento para posterior reúso das águas residuais. Nesse sentido, são apresentadas formas de reúso direto, indireto, planejado e de reciclagem das águas residuais aplicáveis na indústria, na agricultura, em edifícios, na recarga de aqüíferos e em usos urbanos para fins potáveis. Palavras-chave: Água, residuais, reúso, ciclo e reciclagem.

  5. Potabilização de água da chuva por ultrafiltração

    Directory of Open Access Journals (Sweden)

    Taizi Miorando

    Full Text Available RESUMO O objetivo deste trabalho foi utilizar a ultrafiltração (UF para o tratamento de água de chuva, ajustando seus parâmetros físico-químicos e microbiológicos para a potabilidade. Para tal, duas membranas de UF foram testadas em duas pressões diferentes para tratar água de chuva coletada em um prédio da Universidade de Passo Fundo (UPF. A água de chuva apresentou uma moderada concentração de sólidos suspensos, cor, turbidez, matéria orgânica e contaminação por coliformes e uma baixa concentração de nitritos, nitratos e sulfatos. As duas membranas de UF utilizadas (com diâmetro de corte de 4 e 50 kDa permitiram o ajuste desses parâmetros à potabilidade, sendo que a membrana de 50 kDa de fibra oca apresentou fluxos superiores a 130 L.h-1.m-2 (a 2 bar. Logo, a captação de água de chuva e sua UF é uma promissora alternativa para um abastecimento descentralizado de água potável.

  6. The WSB1 gene is involved in pancreatic cancer progression.

    Directory of Open Access Journals (Sweden)

    Cendrine Archange

    Full Text Available BACKGROUND: Pancreatic cancer cells generate metastases because they can survive the stress imposed by the new environment of the host tissue. To mimic this process, pancreatic cancer cells which are not stressed in standard culture conditions are injected into nude mice. Because they develop xenografts, they should have developed adequate stress response. Characterizing that response might provide new strategies to interfere with pancreatic cancer metastasis. METHODOLOGY/PRINCIPAL FINDINGS: In the human pancreatic cancer cell lines Panc-1, Mia-PaCa2, Capan-1, Capan-2 and BxPC3, we used Affymetrix DNA microarrays to compare the expressions of 22.000 genes in vitro and in the corresponding xenografts. We identified 228 genes overexpressed in xenografts and characterized the implication of one of them, WSB1, in the control of apoptosis and cell proliferation. WSB1 generates 3 alternatively spliced transcripts encoding distinct protein isoforms. In xenografts and in human pancreatic tumors, global expression of WSB1 mRNA is modestly increased whereas isoform 3 is strongly overexpressed and isoforms 1 and 2 are down-regulated. Treating Mia-PaCa2 cells with stress-inducing agents induced similar changes. Whereas retrovirus-forced expression of WSB1 isoforms 1 and 2 promoted cell growth and sensitized the cells to gemcitabine- and doxorubicin-induced apoptosis, WSB1 isoform 3 expression reduced cell proliferation and enhanced resistance to apoptosis, showing that stress-induced modulation of WSB1 alternative splicing increases resistance to apoptosis of pancreatic cancer cells. CONCLUSIONS/SIGNIFICANCE: Data on WSB1 regulation support the hypothesis that activation of stress-response mechanisms helps cancer cells establishing metastases and suggest relevance to cancer development of other genes overexpressed in xenografts.

  7. Characterization of two candidate genes, NCoA3 and IRF8, potentially involved in the control of HIV-1 latency

    Directory of Open Access Journals (Sweden)

    Gumez Audrey

    2005-11-01

    Full Text Available Abstract Background The persistence of latent HIV-1 reservoirs is the principal barrier preventing the eradication of HIV-1 infection in patients by current antiretroviral therapy. It is thus crucial to understand the molecular mechanisms involved in the establishment, maintenance and reactivation of HIV-1 latency. Since chromatin remodeling has been implicated in the transcriptional reactivation of the HIV-1 promoter, we assessed the role of the histone deacetylase inhibitor sodium butyrate (NaB on two HIV-1 latently infected cell lines (U1 and ACH-2 gene expression. Results Analysis of microarrays data led us to select two candidate genes: NCoA3 (Nuclear Receptor Coactivator 3, a nuclear receptor coactivator and IRF8 (Interferon Regulatory Factor 8, an interferon regulatory factor. NCoA3 gene expression is upregulated following NaB treatment of latently infected cells whereas IRF8 gene expression is strongly downregulated in the promonocytic cell line following NaB treatment. Their differential expressions were confirmed at the transcriptional and translational levels. Moreover, NCoA3 gene expression was also upregulated after treatment of U1 and ACH-2 cells with phorbol myristyl acetate (PMA but not trichostatin A (TSA and after treatment with NaB of two others HIV-1 latently infected cell lines (OM10.1 and J1.1. IRF8 gene is only expressed in U1 cells and was also downregulated after treatment with PMA or TSA. Functional analyses confirmed that NCoA3 synergizes with Tat to enhance HIV-1 promoter transcription and that IRF8 represses the IRF1-mediated activation through the HIV-1 promoter Interferon-stimulated response element (ISRE. Conclusion These results led us to postulate that NCoA3 could be involved in the transcriptional reactivation of the HIV-1 promoter from latency and that IRF8 may contribute to the maintenance of the latent state in the promonocytic cell line. Implication of these factors in the maintenance or reactivation of the

  8. Exploring genes and pathways involved in migraine

    NARCIS (Netherlands)

    Eising, E.

    2017-01-01

    The research in this thesis was aimed at identifying genes and molecular pathways involved in migraine. To this end, two gene expression analyses were performed in brain tissue obtained from transgenic mouse models for familial hemiplegic migraine (FHM), a monogenic subtype of migraine with aura.

  9. Gestão das perdas de água e energia em sistema de abastecimento de água da Embasa: um estudo dos fatores intervenientes na RMS

    Directory of Open Access Journals (Sweden)

    Renavan Andrade Sobrinho

    Full Text Available RESUMO No Brasil, os sistemas de abastecimento de água ainda apresentam perdas de água e baixa eficiência energética. O presente trabalho objetiva analisar os fatores que têm influenciado na efetividade da gestão das perdas de água e energia em sistemas de abastecimento operados pela Empresa Baiana de Águas e Saneamento (Embasa na região metropolitana de Salvador (RMS. A pesquisa documental e a avaliação dos dados permitiram verificar o comportamento dos indicadores e os resultados dos programas implementados. Os dados evidenciaram que, apesar dos programas, as perdas de água se mantiveram elevadas, e as ações de eficiência energética foram mais eficazes. Os programas implementados foram pontuais, voltados prioritariamente para ações técnico-operacionais, carecendo de um planejamento mais estratégico por parte da empresa. Do estudo, concluiu-se que o sucesso das ações para o uso eficiente da água e energia, obrigatoriamente, exige a superação das abordagens parciais e voltadas para a dimensão técnico-operacional, devendo-se incorporar a noção da complexidade e o caráter intersetorial e integrado.

  10. Epigenetic involvement of Alien/ESET complex in thyroid hormone-mediated repression of E2F1 gene expression and cell proliferation

    International Nuclear Information System (INIS)

    Hong, Wei; Li, Jinru; Wang, Bo; Chen, Linfeng; Niu, Wenyan; Yao, Zhi; Baniahmad, Aria

    2011-01-01

    Highlights: ► Corepressor Alien interacts with histone methyltransferase ESET in vivo. ► Alien/ESET complex is recruited to nTRE of T3-responsive gene by liganded TRβ1. ► ESET-mediated H3K9 methylation is required for liganded TRβ1-repressed transcription. ► ESET is involved in T3-repressed G1/S phase transition and proliferation. -- Abstract: The ligand-bound thyroid hormone receptor (TR) is known to repress via a negative TRE (nTRE) the expression of E2F1, a key transcription factor that controls the G1/S phase transition. Alien has been identified as a novel interacting factor of E2F1 and acts as a corepressor of E2F1. The detailed molecular mechanism by which Alien inhibits E2F1 gene expression remains unclear. Here, we report that the histone H3 lysine 9 (H3K9) methyltransferase (HMT) ESET is an integral component of the corepressor Alien complex and the Alien/ESET complex is recruited to both sites, the E2F1 and the nTRE site of the E2F1 gene while the recruitment to the negative thyroid hormone response element (nTRE) is induced by the ligand-bound TRβ1 within the E2F1 gene promoter. We show that, overexpression of ESET promotes, whereas knockdown of ESET releases, the inhibition of TRβ1-regulated gene transcription upon T3 stimulation; and H3K9 methylation is required for TRβ1-repressed transcription. Furthermore, depletion of ESET impairs thyroid hormone-repressed proliferation as well as the G1/S transition of the cell cycle. Taken together, our data indicate that ESET is involved in TRβ1-mediated transcription repression and provide a molecular basis of thyroid hormone-induced repression of proliferation.

  11. Aquecimento e resfriamento da água, aproximados à forma real

    Directory of Open Access Journals (Sweden)

    Reynaldo Lopes de Oliveira Jr.

    2016-04-01

    Full Text Available http://dx.doi.org/10.5007/2175-7941.2016v33n1p306 O objetivo deste trabalho é mostrar que quando não fazemos muitas simplificações em uma demonstração experimental de calorimetria, podemos levantar em sala de aula questões interessantes. Se aquecermos, com um ebulidor de imersão, uma porção não muito grande de água, observaremos que a temperatura da água continuará aumentando mesmo depois do desligamento da fonte térmica. Se desligamos o ebulidor, que é a fonte de energia térmica deste sistema, como pode a temperatura da água continuar subindo? Esta foi a questão geradora deste trabalho. A partir desta questão e dos dados experimentais coletados no laboratório, elaboramos um modelo matemático que explicasse os dados obtidos. Esta investigação ocorreu em um grupo de seis alunos do ensino médio mediados por um professor de física.

  12. Host genes involved in Agrobacterium-mediated transformation

    NARCIS (Netherlands)

    Soltani, Jalal

    2009-01-01

    Agrobacterium is the nature’s genetic engineer that can transfer genes across the kingdom barriers to both prokaryotic and eukaryotic host cells. The host genes which are involved in Agrobacterium-mediated transformatiom (AMT) are not well known. Here, I studied in a systematic way to identify the

  13. AVALIAÇÃO DA NECESSIDADE DO REÚSO DE ÁGUAS RESIDUAIS

    OpenAIRE

    Costa, Djeson Mateus Alves da; Barros Júnior, Antônio Carlos de

    2007-01-01

    O objetivo desse trabalho foi, por meio uma pesquisa bibliográfica, apresentar um relato sobre a importância do racionamento do consumo de água através de seu reúso, descrever o ciclo global da água e quantificar seus principais reservatórios na terra, identificar as fontes potencialmente poluidoras das reservas hídricas, apresentar os aspectos críticos sobre a disponibilidade e o consumo de água no mundo, mencionar as tecnologias aplicadas no tratamento para posterior reúso das águas residua...

  14. MONITORAMENTO DE SAIS NA ÁGUA E NOS SOLOS IRRIGADOS DO PROJETO VEREDA GRANDE, PB

    Directory of Open Access Journals (Sweden)

    Ladilson de Souza Macêdo

    1998-04-01

    Full Text Available RESUMO Avaliaram-se a variabilidade temporal dos sais na água de irrigação e seus efeitos sobre os solos irrigados do Projeto Vereda Grande, em Boqueirão, PB, através de amostragem aleatória estratificada em três lotes de agricultores. Foram coletadas 66 amostras de solo e 33 de água, no período de janeiro de 1987 a dezembro de 1989, sendo que a água apresentou crescente risco de salinização ao longo do tempo, com elevados níveis de sódio e cloreto - 8,6 e 10,5 mmolc.L-1, respectivamente. A presença de solos Bruno Não Cálcico e Litólicos na área de captação do manancial impede a melhoria da qualidade da água, predispondo os solos irrigados à alcalinização. Dentre as lavouras exploradas pelos irrigantes, apenas as capineiras são moderadamente tolerantes à concentração salina na zona radicular. A presença significativa de bicarbonato (1 a 3,2 mmolc.L-1 na água restringe a prática da irrigação por aspersão devido, principalmente, às condições de alta evaporação, aridez do local e toxidez específica do sódio e cloreto em plantas sensíveis.

  15. Antimicrobial, antiviral and antioxidant activities of "água-mel" from Portugal.

    Science.gov (United States)

    Miguel, Maria G; Faleiro, Leonor; Antunes, Maria D; Aazza, Smail; Duarte, Joana; Silvério, Ana R

    2013-06-01

    gua-mel" is a honey-based product produced in Portugal for ancient times. Several attributes have been reported to "água-mel" particularly in the alleviation of simple symptoms of upper respiratory tract. Samples of "água-mel" from diverse beekeepers from different regions of Portugal were studied in what concerns antimicrobial, antioxidant and antiviral properties. The amounts of phenol and brown pigment were also evaluated and correlated with the antioxidant activities. A great variability on the levels of these compounds was found among samples which were responsible for the variability detected also on the antioxidant activities, independent on the method used. Generally, antioxidant activity correlated better with brown pigments' amount than with phenols' content. The antimicrobial activity found for "água-mel" samples confirm the virtues reported by popular findings. In addition, this work also reveals the antiviral properties of "água-mel" evidenced by a decrease on the infectivity of the Qβ bacteriophage. Copyright © 2013 Elsevier Ltd. All rights reserved.

  16. Global Developmental Gene Programing Involves a Nuclear Form of Fibroblast Growth Factor Receptor-1 (FGFR1.

    Directory of Open Access Journals (Sweden)

    Christopher Terranova

    Full Text Available Genetic studies have placed the Fgfr1 gene at the top of major ontogenic pathways that enable gastrulation, tissue development and organogenesis. Using genome-wide sequencing and loss and gain of function experiments the present investigation reveals a mechanism that underlies global and direct gene regulation by the nuclear form of FGFR1, ensuring that pluripotent Embryonic Stem Cells differentiate into Neuronal Cells in response to Retinoic Acid. Nuclear FGFR1, both alone and with its partner nuclear receptors RXR and Nur77, targets thousands of active genes and controls the expression of pluripotency, homeobox, neuronal and mesodermal genes. Nuclear FGFR1 targets genes in developmental pathways represented by Wnt/β-catenin, CREB, BMP, the cell cycle and cancer-related TP53 pathway, neuroectodermal and mesodermal programing networks, axonal growth and synaptic plasticity pathways. Nuclear FGFR1 targets the consensus sequences of transcription factors known to engage CREB-binding protein, a common coregulator of transcription and established binding partner of nuclear FGFR1. This investigation reveals the role of nuclear FGFR1 as a global genomic programmer of cell, neural and muscle development.

  17. Involvement of Resveratrol and ω-3 Polyunsaturated Fatty Acids on Sirtuin 1 Gene Expression in THP1 Cells.

    Science.gov (United States)

    Tsuchiya, Takafumi; Endo, Ayano; Tsujikado, Kyoko; Inukai, Toshihiko

    2017-10-01

    Resveratrol, a kind of polyphenol, has the potential to activate the longevity gene in several cells, in the same manner as calorie restriction. We investigated the effect of resveratrol and ω-3-line polyunsaturated fatty acid on surtuin 1 (SIRT1) gene expression in human monocytes (THP1) cells. We examined the gene expression of THP1 cells using real-time polymerase chain reaction and Western blotting analysis. Resveratol, eicosapentaenoic acid (EPA) and docosahexaeanoic acid (DHA) as n-3 polyunsaturated fatty acid were added on THP1 cells. We observed the changes in the SIRT1 gene expression in those cells, under various doses of agents and in time courses. Then, we examined the interaction of glucose and mannitol on those agents׳ effect of the gene expression. The concentration range of glucose and mannitol was from 5-20mM, respectively. The SIRT1 gene expression could be defined in 24 and 48 hours both in real-time polymerase chain reaction analysis and in Western blotting. Resveratrol showed SIRT1 gene expression in a dose-dependent manner in the range of 0-20μM in both analyses. Although EPA at 10μM showed marked increase in SIRT1 gene expression compared to control condition in Western blotting, this phenomenon was not in dose-dependent manner. DHA did not exhibit any augmentation of SIRT1 gene expression in a dose-dependent manner in the range of 0-20μM in both analyses. We refined the dose-dependent inhibition of the SIRT1 gene expression within 20mM glucose medium. Although 20mM did not exhibit any inhibition, 10μM resveratrol induced the gene expression compared to control medium. Both 5 and 15mM mannitol medium did not significantly alter basic gene expression and 10μM resveratrol-induced gene expression. The present results suggest that resveratrol and EPA, but not DHA, markedly activated the SIRT1 gene expression in THP1 cells, and that high glucose medium could inhibit the basic gene expression, but not powerful resveratrol-induced gene

  18. Estudo comparativo do resfriamento de laranja valência com ar forçado e com água

    OpenAIRE

    Teruel,Bárbara; Cortez,Luís; Neves Filho,Lincoln

    2003-01-01

    Este trabalho apresenta um estudo para comparar o resfriamento rápido de laranja Valência (Citrus sinensis O.), com ar forçado e com água gelada. O sistema de resfriamento rápido com ar forçado operou com um fluxo de ar de 1.933m³/h (3 l/s por kg de produto resfriado), a uma temperatura de 1ºC e UR=88,4 ± 2,0%, com uma velocidade do ar em torno de 1m/s. Nos experimentos com água gelada foi utilizado um sistema de imersão, com uma capacidade de 0,23m³ de água a uma temperatura de aproximadamen...

  19. Acetate alters expression of genes involved in beige adipogenesis in 3T3-L1 cells and obese KK-Ay mice

    Science.gov (United States)

    Hanatani, Satoko; Motoshima, Hiroyuki; Takaki, Yuki; Kawasaki, Shuji; Igata, Motoyuki; Matsumura, Takeshi; Kondo, Tatsuya; Senokuchi, Takafumi; Ishii, Norio; Kawashima, Junji; Kukidome, Daisuke; Shimoda, Seiya; Nishikawa, Takeshi; Araki, Eiichi

    2016-01-01

    The induction of beige adipogenesis within white adipose tissue, known as “browning”, has received attention as a novel potential anti-obesity strategy. The expression of some characteristic genes including PR domain containing 16 is induced during the browning process. Although acetate has been reported to suppress weight gain in both rodents and humans, its potential effects on beige adipogenesis in white adipose tissue have not been fully characterized. We examined the effects of acetate treatment on 3T3-L1 cells and in obese diabetic KK-Ay mice. The mRNA expression levels of genes involved in beige adipocyte differentiation and genes selectively expressed in beige adipocytes were significantly elevated in both 3T3-L1 cells incubated with 1.0 mM acetate and the visceral white adipose tissue from mice treated with 0.6% acetate for 16 weeks. In KK-Ay mice, acetate reduced the food efficiency ratio and increased the whole-body oxygen consumption rate. Additionally, reduction of adipocyte size and uncoupling protein 1-positive adipocytes and interstitial areas with multilocular adipocytes appeared in the visceral white adipose tissue of acetate-treated mice, suggesting that acetate induced initial changes of “browning”. In conclusion, acetate alters the expression of genes involved in beige adipogenesis and might represent a potential therapeutic agent to combat obesity. PMID:27895388

  20. A genetic screen for modifiers of Drosophila caspase Dcp-1 reveals caspase involvement in autophagy and novel caspase-related genes

    Directory of Open Access Journals (Sweden)

    Ahnn Joohong

    2010-01-01

    Full Text Available Abstract Background Caspases are cysteine proteases with essential functions in the apoptotic pathway; their proteolytic activity toward various substrates is associated with the morphological changes of cells. Recent reports have described non-apoptotic functions of caspases, including autophagy. In this report, we searched for novel modifiers of the phenotype of Dcp-1 gain-of-function (GF animals by screening promoter element- inserted Drosophila melanogaster lines (EP lines. Results We screened ~15,000 EP lines and identified 72 Dcp-1-interacting genes that were classified into 10 groups based on their functions and pathways: 4 apoptosis signaling genes, 10 autophagy genes, 5 insulin/IGF and TOR signaling pathway genes, 6 MAP kinase and JNK signaling pathway genes, 4 ecdysone signaling genes, 6 ubiquitination genes, 11 various developmental signaling genes, 12 transcription factors, 3 translation factors, and 11 other unclassified genes including 5 functionally undefined genes. Among them, insulin/IGF and TOR signaling pathway, MAP kinase and JNK signaling pathway, and ecdysone signaling are known to be involved in autophagy. Together with the identification of autophagy genes, the results of our screen suggest that autophagy counteracts Dcp-1-induced apoptosis. Consistent with this idea, we show that expression of eGFP-Atg5 rescued the eye phenotype caused by Dcp-1 GF. Paradoxically, we found that over-expression of full-length Dcp-1 induced autophagy, as Atg8b-GFP, an indicator of autophagy, was increased in the eye imaginal discs and in the S2 cell line. Taken together, these data suggest that autophagy suppresses Dcp-1-mediated apoptotic cell death, whereas Dcp-1 positively regulates autophagy, possibly through feedback regulation. Conclusions We identified a number of Dcp-1 modifiers that genetically interact with Dcp-1-induced cell death. Our results showing that Dcp-1 and autophagy-related genes influence each other will aid future

  1. The Hypocholesterolemic Effect of Germinated Brown Rice Involves the Upregulation of the Apolipoprotein A1 and Low-Density Lipoprotein Receptor Genes

    Directory of Open Access Journals (Sweden)

    Mustapha Umar Imam

    2013-01-01

    Full Text Available Germinated brown rice (GBR is rich in bioactive compounds, which confer GBR with many functional properties. Evidence of its hypocholesterolemic effects is emerging, but the exact mechanisms of action and bioactive compounds involved have not been fully documented. Using type 2 diabetic rats, we studied the effects of white rice, GBR, and brown rice (BR on lipid profile and on the regulation of selected genes involved in cholesterol metabolism. Our results showed that the upregulation of apolipoprotein A1 and low-density lipoprotein receptor genes was involved in the hypocholesterolemic effects of GBR. Additionally, in vitro studies using HEPG2 cells showed that acylated steryl glycoside, gamma amino butyric acid, and oryzanol and phenolic extracts of GBR contribute to the nutrigenomic regulation of these genes. Transcriptional and nontranscriptional mechanisms are likely involved in the overall hypocholesterolemic effects of GBR suggesting that it may have an impact on the prevention and/or management of hypercholesterolemia due to a wide variety of metabolic perturbations. However, there is need to conduct long-term clinical trials to determine the clinical relevance of the hypocholesterolemic effects of GBR determined through animal studies.

  2. The role of genes involved in neuroplasticity and neurogenesis in the observation of a gene-environment interaction (GxE) in schizophrenia.

    Science.gov (United States)

    Le Strat, Yann; Ramoz, Nicolas; Gorwood, Philip

    2009-05-01

    Schizophrenia is a multifactorial disease characterized by a high heritability. Several candidate genes have been suggested, with the strongest evidences for genes encoding dystrobrevin binding protein 1 (DTNBP1), neuregulin 1 (NRG1), neuregulin 1 receptor (ERBB4) and disrupted in schizophrenia 1 (DISC1), as well as several neurotrophic factors. These genes are involved in neuronal plasticity and play also a role in adult neurogenesis. Therefore, the genetic basis of schizophrenia could involve different factors more or less specifically required for neuroplasticity, including the synapse maturation, potentiation and plasticity as well as neurogenesis. Following the model of Knudson in tumors, we propose a two-hit hypothesis of schizophrenia. In this model of gene-environment interaction, a variant in a gene related to neurogenesis is transmitted to the descent (first hit), and, secondarily, an environmental factor occurs during the development of the central nervous system (second hit). Both of these vulnerability and trigger factors are probably necessary to generate a deficit in neurogenesis and therefore to cause schizophrenia. The literature supporting this gene x environment hypothesis is reviewed, with emphasis on some molecular pathways, raising the possibility to propose more specific molecular medicine.

  3. APROVEITAMENTO, CAPTAÇÃO E (RE USO DAS ÁGUAS PLUVIAIS NA ARQUITETURA

    Directory of Open Access Journals (Sweden)

    Melhores Trabalhos .

    2015-02-01

    Full Text Available O presente artigo tem a intenção de abordar a captação e o (re uso das águas pluviais com ênfase na arquitetura sustentável, tendo como finalidade básica a conscientização do consumo exagerado de água potável. O aproveitamento da água da chuva é uma das alternativas principais para a redução da escassez da água, conservação dos lençóis freáticos e para a colaboração no sistema de drenagem urbana. Dessa forma, a pesquisa apresentará informações referentes às várias formas e sistemas de captação de água pluvial no intuito de fortalecer a responsabilidade ambiental e social de cada usuário junto ao meio ambiente através de alternativas arquitetônicas eficientes no aproveitamento dessas águas.

  4. Avaliação da concentração de flúor e do consumo de água mineral

    Directory of Open Access Journals (Sweden)

    Ramires Irene

    2004-01-01

    Full Text Available OBJETIVO: Considerando a água como importante fonte de ingestão de flúor, o aumento da prevalência de fluorose dentária e do consumo de água mineral, o estudo realizado teve como objetivo avaliar o consumo de água mineral e sua concentração de flúor. MÉTODOS: A pesquisa foi realizada nos bairros do município de Bauru, SP. Foram visitadas mil residências que compunham uma amostra estratificada, obtida por conglomerados. Cada conglomerado correspondia a um quarteirão. Para efeito de sorteio os quarteirões foram numerados dentro das 17 regiões estabelecidas no plano diretor do município. Para coleta das amostras de água mineral foram usados frascos plásticos de 50 ml, previamente identificados. Na análise de flúor utilizou-se eletrodo íon sensível (Orion 9609 após tamponamento com TISAB II. Informações a respeito do consumo de água mineral foram obtidas com a aplicação de um questionário. RESULTADOS: Constatou-se que cerca de 29,7% da população do município consome água mineral. Nas 260 amostras analisadas, das 29 diferentes águas, a concentração de flúor variou de 0,045 a 1,515 mg/l. Em uma das águas, constava no rótulo o valor de 0,220 mg/l, mas na análise constatou-se 1,515 mg/l de flúor. Além disso, algumas águas não traziam especificado a concentração de flúor; e, na análise constataram-se valores variando entre 0,049 e 0,924 mg/l. CONCLUSÕES: Os resultados mostraram uma grande variação na concentração de flúor e reforçam a importância do controle dessas águas por parte da vigilância sanitária.

  5. Differential Gene Expression by Lactobacillus plantarum WCFS1 in Response to Phenolic Compounds Reveals New Genes Involved in Tannin Degradation.

    Science.gov (United States)

    Reverón, Inés; Jiménez, Natalia; Curiel, José Antonio; Peñas, Elena; López de Felipe, Félix; de Las Rivas, Blanca; Muñoz, Rosario

    2017-04-01

    Lactobacillus plantarum is a lactic acid bacterium that can degrade food tannins by the successive action of tannase and gallate decarboxylase enzymes. In the L. plantarum genome, the gene encoding the catalytic subunit of gallate decarboxylase ( lpdC , or lp_2945 ) is only 6.5 kb distant from the gene encoding inducible tannase ( L. plantarum tanB [ tanB Lp ], or lp_2956 ). This genomic context suggests concomitant activity and regulation of both enzymatic activities. Reverse transcription analysis revealed that subunits B ( lpdB , or lp_0271 ) and D ( lpdD , or lp_0272 ) of the gallate decarboxylase are cotranscribed, whereas subunit C ( lpdC , or lp_2945 ) is cotranscribed with a gene encoding a transport protein ( gacP , or lp_2943 ). In contrast, the tannase gene is transcribed as a monocistronic mRNA. Investigation of knockout mutations of genes located in this chromosomal region indicated that only mutants of the gallate decarboxylase (subunits B and C), tannase, GacP transport protein, and TanR transcriptional regulator ( lp_2942 ) genes exhibited altered tannin metabolism. The expression profile of genes involved in tannin metabolism was also analyzed in these mutants in the presence of methyl gallate and gallic acid. It is noteworthy that inactivation of tanR suppresses the induction of all genes overexpressed in the presence of methyl gallate and gallic acid. This transcriptional regulator was also induced in the presence of other phenolic compounds, such as kaempferol and myricetin. This study complements the catalog of L. plantarum expression profiles responsive to phenolic compounds, which enable this bacterium to adapt to a plant food environment. IMPORTANCE Lactobacillus plantarum is a bacterial species frequently found in the fermentation of vegetables when tannins are present. L. plantarum strains degrade tannins to the less-toxic pyrogallol by the successive action of tannase and gallate decarboxylase enzymes. The genes encoding these enzymes are

  6. Bioinformatics Analysis Reveals Genes Involved in the Pathogenesis of Ameloblastoma and Keratocystic Odontogenic Tumor.

    Science.gov (United States)

    Santos, Eliane Macedo Sobrinho; Santos, Hércules Otacílio; Dos Santos Dias, Ivoneth; Santos, Sérgio Henrique; Batista de Paula, Alfredo Maurício; Feltenberger, John David; Sena Guimarães, André Luiz; Farias, Lucyana Conceição

    2016-01-01

    Pathogenesis of odontogenic tumors is not well known. It is important to identify genetic deregulations and molecular alterations. This study aimed to investigate, through bioinformatic analysis, the possible genes involved in the pathogenesis of ameloblastoma (AM) and keratocystic odontogenic tumor (KCOT). Genes involved in the pathogenesis of AM and KCOT were identified in GeneCards. Gene list was expanded, and the gene interactions network was mapped using the STRING software. "Weighted number of links" (WNL) was calculated to identify "leader genes" (highest WNL). Genes were ranked by K-means method and Kruskal-Wallis test was used (Preview data was used to corroborate the bioinformatics data. CDK1 was identified as leader gene for AM. In KCOT group, results show PCNA and TP53 . Both tumors exhibit a power law behavior. Our topological analysis suggested leader genes possibly important in the pathogenesis of AM and KCOT, by clustering coefficient calculated for both odontogenic tumors (0.028 for AM, zero for KCOT). The results obtained in the scatter diagram suggest an important relationship of these genes with the molecular processes involved in AM and KCOT. Ontological analysis for both AM and KCOT demonstrated different mechanisms. Bioinformatics analyzes were confirmed through literature review. These results may suggest the involvement of promising genes for a better understanding of the pathogenesis of AM and KCOT.

  7. Epigenetic involvement of Alien/ESET complex in thyroid hormone-mediated repression of E2F1 gene expression and cell proliferation

    Energy Technology Data Exchange (ETDEWEB)

    Hong, Wei, E-mail: hongwei@tijmu.edu.cn [Department of Immunology, Tianjin Medical University, 300070 Tianjin (China); College of Basic Medicine, Tianjin Medical University, 300070 Tianjin (China); Li, Jinru; Wang, Bo [College of Basic Medicine, Tianjin Medical University, 300070 Tianjin (China); Chen, Linfeng [Department of Medical Oncology, Harvard Medical School, Dana Farber Cancer Institute, Boston, 02115 MA (United States); Niu, Wenyan; Yao, Zhi [Department of Immunology, Tianjin Medical University, 300070 Tianjin (China); Baniahmad, Aria, E-mail: aban@mti.uni-jena.de [Institute for Human Genetics, Jena University Hospital, 07740 Jena (Germany)

    2011-12-02

    Highlights: Black-Right-Pointing-Pointer Corepressor Alien interacts with histone methyltransferase ESET in vivo. Black-Right-Pointing-Pointer Alien/ESET complex is recruited to nTRE of T3-responsive gene by liganded TR{beta}1. Black-Right-Pointing-Pointer ESET-mediated H3K9 methylation is required for liganded TR{beta}1-repressed transcription. Black-Right-Pointing-Pointer ESET is involved in T3-repressed G1/S phase transition and proliferation. -- Abstract: The ligand-bound thyroid hormone receptor (TR) is known to repress via a negative TRE (nTRE) the expression of E2F1, a key transcription factor that controls the G1/S phase transition. Alien has been identified as a novel interacting factor of E2F1 and acts as a corepressor of E2F1. The detailed molecular mechanism by which Alien inhibits E2F1 gene expression remains unclear. Here, we report that the histone H3 lysine 9 (H3K9) methyltransferase (HMT) ESET is an integral component of the corepressor Alien complex and the Alien/ESET complex is recruited to both sites, the E2F1 and the nTRE site of the E2F1 gene while the recruitment to the negative thyroid hormone response element (nTRE) is induced by the ligand-bound TR{beta}1 within the E2F1 gene promoter. We show that, overexpression of ESET promotes, whereas knockdown of ESET releases, the inhibition of TR{beta}1-regulated gene transcription upon T3 stimulation; and H3K9 methylation is required for TR{beta}1-repressed transcription. Furthermore, depletion of ESET impairs thyroid hormone-repressed proliferation as well as the G1/S transition of the cell cycle. Taken together, our data indicate that ESET is involved in TR{beta}1-mediated transcription repression and provide a molecular basis of thyroid hormone-induced repression of proliferation.

  8. The YJR127C/ZMS1 gene product is involved in glycerol-based respiratory growth of the yeast Saccharomyces cerevisiae.

    Science.gov (United States)

    Lu, Lin; Roberts, George G; Oszust, Cynthia; Hudson, Alan P

    2005-10-01

    A putative yeast mitochondrial upstream activating sequence (UAS) was used in a one-hybrid screening procedure that identified the YJR127C ORF on chromosome X. This gene was previously designated ZMS1 and is listed as a transcription factor on the SGD website. Real time RT-PCR assays showed that expression of YJR127C/ZMS1 was glucose-repressible, and a deletion mutant for the gene showed a growth defect on glycerol-based but not on glucose- or ethanol-based medium. Real time RT-PCR analyses identified severely attenuated transcript levels from GUT1 and GUT2 to be the source of that growth defect, the products of GUT1 and GUT2 are required for glycerol utilization. mRNA levels from a large group of mitochondria- and respiration-related nuclear genes also were shown to be attenuated in the deletion mutant. Importantly, transcript levels from the mitochondrial OLI1 gene, which has an associated organellar UAS, were attenuated in the DeltaYJR127C mutant during glycerol-based growth, but those from COX3 (OXI2), which lacks an associated mitochondrial UAS, were not. Transcriptome analysis of the glycerol-grown deletion mutant showed that genes in several metabolic and other categories are affected by loss of this gene product, including protein transport, signal transduction, and others. Thus, the product of YJR127C/ZMS1 is involved in transcriptional control for genes in both cellular genetic compartments, many of which specify products required for glycerol-based growth, respiration, and other functions.

  9. Sistema alternativo de tratamento de água em escala descentralizada

    OpenAIRE

    Franco, Camila Silva

    2015-01-01

    A inexistência de um sistema de tratamento e distribuição de água em áreas remotas leva a população ao consumo de água de baixa qualidade, em função das ações antrópicas no nível das bacias hidrográficas. O desenvolvimento de sistemas descentralizados, que contemplem as distâncias entre residências em áreas escassamente povoadas pode proporcionar água potável, de maneira fácil, autossustentável e a baixo custo. Nesse contexto, objetivou-se o desenvolvimento de uma Estação de Tratamento de Águ...

  10. Polymorphisms in genes involved in GH1 release and their association with breast cancer risk.

    Science.gov (United States)

    Wagner, Kerstin; Hemminki, Kari; Grzybowska, Ewa; Klaes, Rüdiger; Burwinkel, Barbara; Bugert, Peter; Schmutzler, Rita K; Wappenschmidt, Barbara; Butkiewicz, Dorota; Pamula, Jolanta; Pekala, Wioletta; Försti, Asta

    2006-09-01

    The regulation of growth hormone 1 (GH1) and insulin-like-growth factor-1 (IGF-1) release is under the influence of three pituitary hormones [growth hormone releasing hormone (GHRH), ghrelin (GHRL) and somatostatin (SST)], which act in an autocrine/paracrine fashion in the breast. By binding to their respective receptors, they control cell proliferation, differentiation and apoptosis in a GH1/IGF-1-dependent manner. We investigated single nucleotide polymorphisms (SNPs) in the GHRH, GHRHR, GHRL, GHSR, SST and SSTR2 gene regions in a Polish and a German cohort of 798 breast cancer cases and 1011 controls. Our study revealed an association of a novel TC repeat polymorphism in the SST promoter with a decreased breast cancer risk in the Polish study population [odds ratio (OR), 0.65; 95% confidence interval (CI), 0.44-0.96]. The closely linked SNP IVS1 A+46G showed the same trend. For both polymorphisms the association was stronger in women above the age of 50 (OR, 0.33; 95% CI, 0.14-0.76 and OR, 0.39; 95% CI, 0.18-0.87, respectively). The protective effect of these polymorphisms was confirmed in a haplotype analysis among women above 50 years of age and carrying the two variant alleles (OR, 0.37; 95% CI, 0.17-0.80). In the independent German population, we observed slightly decreased ORs among women above the age of 50 years. In the SSTR2 gene, carriers of the promoter 21/21 TG repeat genotype were at a decreased breast cancer risk (OR, 0.62; 95% CI, 0.41-0.94) compared to carriers of the other genotypes in the Polish population. Furthermore, we identified a protective effect of the GHRHR C-261T SNP in both populations (joint analysis CT+TT versus CC: OR, 0.80; 95% CI, 0.65-0.99). This effect was carried by a haplotype containing the protective allele. Thus, our study concludes a possible protective influence of distinct polymorphisms in genes involved in GH1 release on breast cancer risk.

  11. Fox (forkhead) genes are involved in the dorso-ventral patterning of the Xenopus mesoderm.

    Science.gov (United States)

    El-Hodiri, H; Bhatia-Dey, N; Kenyon, K; Ault, K; Dirksen, M; Jamrich, M

    2001-01-01

    Fox (forkhead/winged helix) genes encode a family of transcription factors that are involved in embryonic pattern formation, regulation of tissue specific gene expression and tumorigenesis. Several of them are transcribed during Xenopus embryogenesis and are important for the patterning of ectoderm, mesoderm and endoderm. We have isolated three forkhead genes that are activated during gastrulation and play an important role in the dorso-ventral patterning of the mesoderm. XFKH1 (FoxA4b), the first vertebrate forkhead gene to be implicated in embryonic pattern formation, is expressed in the Spemann-Mangold organizer region and later in the embryonic notochord. XFKH7, the Xenopus orthologue of the murine Mfh1(Foxc2), is expressed in the presomitic mesoderm, but not in the notochord or lateral plate mesoderm. Finally, XFD-13'(FoxF1b)1 is expressed in the lateral plate mesoderm, but not in the notochord or presomitic mesoderm. Expression pattern and functional experiments indicate that these three forkhead genes are involved in the dorso-ventral patterning of the mesoderm.

  12. A Morpholino-based screen to identify novel genes involved in craniofacial morphogenesis

    Science.gov (United States)

    Melvin, Vida Senkus; Feng, Weiguo; Hernandez-Lagunas, Laura; Artinger, Kristin Bruk; Williams, Trevor

    2014-01-01

    BACKGROUND The regulatory mechanisms underpinning facial development are conserved between diverse species. Therefore, results from model systems provide insight into the genetic causes of human craniofacial defects. Previously, we generated a comprehensive dataset examining gene expression during development and fusion of the mouse facial prominences. Here, we used this resource to identify genes that have dynamic expression patterns in the facial prominences, but for which only limited information exists concerning developmental function. RESULTS This set of ~80 genes was used for a high throughput functional analysis in the zebrafish system using Morpholino gene knockdown technology. This screen revealed three classes of cranial cartilage phenotypes depending upon whether knockdown of the gene affected the neurocranium, viscerocranium, or both. The targeted genes that produced consistent phenotypes encoded proteins linked to transcription (meis1, meis2a, tshz2, vgll4l), signaling (pkdcc, vlk, macc1, wu:fb16h09), and extracellular matrix function (smoc2). The majority of these phenotypes were not altered by reduction of p53 levels, demonstrating that both p53 dependent and independent mechanisms were involved in the craniofacial abnormalities. CONCLUSIONS This Morpholino-based screen highlights new genes involved in development of the zebrafish craniofacial skeleton with wider relevance to formation of the face in other species, particularly mouse and human. PMID:23559552

  13. Gestão das perdas de água e energia em sistemas de abastecimento de água da Embasa: Um estudo dos fatores intervenientes na RMS

    Directory of Open Access Journals (Sweden)

    Renavan Andrade Sobrinho

    2014-08-01

    Full Text Available No Brasil, apesar das experiências de diversos programas e projetos para o controle das perdas de água e de eficiência energética, os prestadores de serviços públicos de abastecimento de água e de esgotamento sanitário ainda apresentam números elevados em relação aos padrões internacionais de países desenvolvidos. A Embasa como um dos principais prestadores de serviços públicos do País, também apresenta essa dura realidade, principalmente considerando os seus grandes centros urbanos, e, em especial, Salvador, a capital do estado. A presente pesquisa objetivou analisar os fatores técnicos, operacionais, administrativos e gerenciais que têm influenciado na efetividade da gestão das perdas de água e energia em sistemas de abastecimento operados pela Embasa na RMS. A investigação foi guiada por um marco conceitual de referência que possibilitou a identificação de seis categorias analíticas, a saber: fatores técnicos, operacionais, administrativos, gerenciais, ambientais e sociais. Para cada categoria foram identificadas variáveis capazes de auxiliar no estudo dos fenômenos da gestão eficiente da água e energia. Para o desenvolvimento da presente pesquisa foram utilizadas técnicas de investigação tanto qualitativas como quantitativas, valendo-se das possibilidades da triangulação de informações e dados que esse procedimento possibilita. A análise documental dos programas de controle de perdas de água e eficiência energética já implementados pela Embasa na RMS permitiu entender as ações planejadas ao longo do tempo e seus reflexos na gestão das perdas. Foram avaliados durante o período de 1996-2011 todos os programas e projetos para controle das perdas de água desenvolvidos para a Região Metropolitana de Salvador e, também, o programa de eficientização energética da Estação Elevatória Teodoro Sampaio/R1 Duna. Foiavaliado o planejamento estratégico da Embasa e verificado os objetivos, metas

  14. Bioinformatic analysis of Msx1 and Msx2 involved in craniofacial development.

    Science.gov (United States)

    Dai, Jiewen; Mou, Zhifang; Shen, Shunyao; Dong, Yuefu; Yang, Tong; Shen, Steve Guofang

    2014-01-01

    Msx1 and Msx2 were revealed to be candidate genes for some craniofacial deformities, such as cleft lip with/without cleft palate (CL/P) and craniosynostosis. Many other genes were demonstrated to have a cross-talk with MSX genes in causing these defects. However, there is no systematic evaluation for these MSX gene-related factors. In this study, we performed systematic bioinformatic analysis for MSX genes by combining using GeneDecks, DAVID, and STRING database, and the results showed that there were numerous genes related to MSX genes, such as Irf6, TP63, Dlx2, Dlx5, Pax3, Pax9, Bmp4, Tgf-beta2, and Tgf-beta3 that have been demonstrated to be involved in CL/P, and Fgfr2, Fgfr1, Fgfr3, and Twist1 that were involved in craniosynostosis. Many of these genes could be enriched into different gene groups involved in different signaling ways, different craniofacial deformities, and different biological process. These findings could make us analyze the function of MSX gens in a gene network. In addition, our findings showed that Sumo, a novel gene whose polymorphisms were demonstrated to be associated with nonsyndromic CL/P by genome-wide association study, has protein-protein interaction with MSX1, which may offer us an alternative method to perform bioinformatic analysis for genes found by genome-wide association study and can make us predict the disrupted protein function due to the mutation in a gene DNA sequence. These findings may guide us to perform further functional studies in the future.

  15. Contributos para a Gestão de Energia em Serviços de Águas

    OpenAIRE

    Boavida, Sara Maria Quaresma

    2016-01-01

    O nexo água-energia reveste-se de grande importância nos serviços de águas. A água é o seu core business e a energia é essencial às suas atividades. O crescente ritmo de urbanização, a escassez de água e a legislação mais exigente no tratamento de águas residuais fazem prever um aumento das necessidades energéticas das entidades gestoras. O aumento da fatura energética nas entidades gestoras compromete a sua sustentabilidade financeira e as emissões de gases de efeito de estufa decorrentes do...

  16. Stably Expressed Genes Involved in Basic Cellular Functions.

    Directory of Open Access Journals (Sweden)

    Kejian Wang

    Full Text Available Stably Expressed Genes (SEGs whose expression varies within a narrow range may be involved in core cellular processes necessary for basic functions. To identify such genes, we re-analyzed existing RNA-Seq gene expression profiles across 11 organs at 4 developmental stages (from immature to old age in both sexes of F344 rats (n = 4/group; 320 samples. Expression changes (calculated as the maximum expression / minimum expression for each gene of >19000 genes across organs, ages, and sexes ranged from 2.35 to >109-fold, with a median of 165-fold. The expression of 278 SEGs was found to vary ≤4-fold and these genes were significantly involved in protein catabolism (proteasome and ubiquitination, RNA transport, protein processing, and the spliceosome. Such stability of expression was further validated in human samples where the expression variability of the homologous human SEGs was significantly lower than that of other genes in the human genome. It was also found that the homologous human SEGs were generally less subject to non-synonymous mutation than other genes, as would be expected of stably expressed genes. We also found that knockout of SEG homologs in mouse models was more likely to cause complete preweaning lethality than non-SEG homologs, corroborating the fundamental roles played by SEGs in biological development. Such stably expressed genes and pathways across life-stages suggest that tight control of these processes is important in basic cellular functions and that perturbation by endogenous (e.g., genetics or exogenous agents (e.g., drugs, environmental factors may cause serious adverse effects.

  17. Genes related to antioxidant metabolism are involved in Methylobacterium mesophilicum-soybean interaction.

    Science.gov (United States)

    Araújo, Welington Luiz; Santos, Daiene Souza; Dini-Andreote, Francisco; Salgueiro-Londoño, Jennifer Katherine; Camargo-Neves, Aline Aparecida; Andreote, Fernando Dini; Dourado, Manuella Nóbrega

    2015-10-01

    The genus Methylobacterium is composed of pink-pigmented methylotrophic bacterial species that are widespread in natural environments, such as soils, stream water and plants. When in association with plants, this genus colonizes the host plant epiphytically and/or endophytically. This association is known to promote plant growth, induce plant systemic resistance and inhibit plant infection by phytopathogens. In the present study, we focused on evaluating the colonization of soybean seedling-roots by Methylobacterium mesophilicum strain SR1.6/6. We focused on the identification of the key genes involved in the initial step of soybean colonization by methylotrophic bacteria, which includes the plant exudate recognition and adaptation by planktonic bacteria. Visualization by scanning electron microscopy revealed that M. mesophilicum SR1.6/6 colonizes soybean roots surface effectively at 48 h after inoculation, suggesting a mechanism for root recognition and adaptation before this period. The colonization proceeds by the development of a mature biofilm on roots at 96 h after inoculation. Transcriptomic analysis of the planktonic bacteria (with plant) revealed the expression of several genes involved in membrane transport, thus confirming an initial metabolic activation of bacterial responses when in the presence of plant root exudates. Moreover, antioxidant genes were mostly expressed during the interaction with the plant exudates. Further evaluation of stress- and methylotrophic-related genes expression by qPCR showed that glutathione peroxidase and glutathione synthetase genes were up-regulated during the Methylobacterium-soybean interaction. These findings support that glutathione (GSH) is potentially a key molecule involved in cellular detoxification during plant root colonization. In addition to methylotrophic metabolism, antioxidant genes, mainly glutathione-related genes, play a key role during soybean exudate recognition and adaptation, the first step in

  18. Occupational Styrene Exposure Induces Stress-Responsive Genes Involved in Cytoprotective and Cytotoxic Activities

    Science.gov (United States)

    Strafella, Elisabetta; Bracci, Massimo; Staffolani, Sara; Manzella, Nicola; Giantomasi, Daniele; Valentino, Matteo; Amati, Monica; Tomasetti, Marco; Santarelli, Lory

    2013-01-01

    Objective The aim of this study was to evaluate the expression of a panel of genes involved in toxicology in response to styrene exposure at levels below the occupational standard setting. Methods Workers in a fiber glass boat industry were evaluated for a panel of stress- and toxicity-related genes and associated with biochemical parameters related to hepatic injury. Urinary styrene metabolites (MA+PGA) of subjects and environmental sampling data collected for air at workplace were used to estimate styrene exposure. Results Expression array analysis revealed massive upregulation of genes encoding stress-responsive proteins (HSPA1L, EGR1, IL-6, IL-1β, TNSF10 and TNFα) in the styrene-exposed group; the levels of cytokines released were further confirmed in serum. The exposed workers were then stratified by styrene exposure levels. EGR1 gene upregulation paralleled the expression and transcriptional protein levels of IL-6, TNSF10 and TNFα in styrene exposed workers, even at low level. The activation of the EGR1 pathway observed at low-styrene exposure was associated with a slight increase of hepatic markers found in highly exposed subjects, even though they were within normal range. The ALT and AST levels were not affected by alcohol consumption, and positively correlated with urinary styrene metabolites as evaluated by multiple regression analysis. Conclusion The pro-inflammatory cytokines IL-6 and TNFα are the primary mediators of processes involved in the hepatic injury response and regeneration. Here, we show that styrene induced stress responsive genes involved in cytoprotection and cytotoxicity at low-exposure, that proceed to a mild subclinical hepatic toxicity at high-styrene exposure. PMID:24086524

  19. Identification and analysis of novel genes involved in gravitropism of Arabidopsis thaliana.

    Science.gov (United States)

    Morita, Miyo T.; Tasaka, Masao; Masatoshi Taniguchi, .

    2012-07-01

    Gravitropism is a continuous control with regard to the orientation and juxtaposition of the various parts of the plant body in response to gravity. In higher plants, the relative directional change of gravity is mainly suscepted in specialized cells called statocytes, followed by signal conversion from physical information into physiological information within the statocytes. We have studied the early process of shoot gravitropism, gravity sensing and signaling process, mainly by molecular genetic approach. In Arabidopsis shoot, statocytes are the endodermal cells. sgr1/scarcrow (scr) and sgr7/short-root (shr) mutants fail to form the endodermis and to respond to gravity in their inflorescence stems. Since both SGR1/SCR and SGR7/SHR are transcriptional factors, at least a subset of their downstream genes can be expected to be involved in gravitropism. In addition, eal1 (endodermal-amyloplast less 1), which exhibits no gravitropism in inflorescence stem but retains ability to form endodermis, is a hypomorphic allele of sgr7/shr. Take advantage of these mutants, we performed DNA microarray analysis and compared gene expression profiles between wild type and the mutants. We found that approx. 40 genes were commonly down-regulated in these mutants and termed them DGE (DOWN-REGULATED GENE IN EAL1) genes. DGE1 has sequence similarity to Oryza sativa LAZY1 that is involved in shoot gravitropism of rice. DGE2 has a short region homologous to DGE1. DTL (DGE TWO-LIKE}) that has 54% identity to DGE2 is found in Arabidopsis genome. All three genes are conserved in angiosperm but have no known functional domains or motifs. We analyzed T-DNA insertion for these genes in single or multiple combinations. In dge1 dge2 dtl triple mutant, gravitropic response of shoot, hypocotyl and root dramatically reduced. Now we are carrying out further physiological and molecular genetic analysis of the triple mutant.

  20. Coverage analysis of lists of genes involved in heterogeneous ...

    Indian Academy of Sciences (India)

    Genes involved in myopathies: 82 genes, based on the disease groups ... 605517 Muscular dystrophy-dystroglycanopathy (congenital with brain and eye ..... Epilepsy, X-linked, with variable learning disabilities and behavior disorders. 300491.

  1. Viabilidade de Vibrio cholerae O1 em diferentes tipos de águas em condições experimentais Viability of Vibrio cholerae O1 in different types of water under experimental conditions

    Directory of Open Access Journals (Sweden)

    Joseli Maria da Rocha Nogueira

    2002-10-01

    Full Text Available A natureza endêmica e sazonal da cólera depende da sobrevivência de Vibrio cholerae O1 em estado viável, mas não necessariamente cultivável em nichos ambientais aquáticos durante períodos interepidêmicos, sendo de suma importância o estudo da sobrevivência deste microrganismo nesses locais. Para tal, foram coletadas, semanalmente, alíquotas de água pertencentes a duas lagoas e dois rios do Estado do Rio de Janeiro. Esses volumes foram divididos em duas porções idênticas, uma das quais foi autoclavada. Uma diluição padronizada de V. cholerae O1 Inaba e de V. cholerae O1 Ogawa, foi inoculada em três alíquotas de 100ml dessas diferentes águas e mantidas em diferentes temperaturas. A sobrevivência desses microorganismos no âmbito aquático sob esses diferentes fatores foi então analisada. Os resultados demonstraram que o V. cholerae sorogrupo O1, independente do sorotipo, é capaz de se manter em água com salinidade abaixo de 0,5‰ e em diferentes temperaturas, por períodos suficientes para sua disseminação através de "corpos d'água", demonstrando a necessidade de monitoramento constante em áreas de possível contaminação, principalmente onde a água é utilizada para o consumo, evitando assim, a disseminação da doença para as populações próximas a esses ambientes.The endemic and seasonal nature of cholera depends upon the survival of Vibrio cholerae O1 in a viable but not necessarily culturable state in ecological niches in aquatic environments during inter-epidemic periods, and investigation on the survival of this microorganism in such sites is therefore of the utmost importance. Weekly water aliquots were thus taken from 2 ponds and 2 rivers in the State of Rio de Janeiro. The samples were divided into two identical portions, one of which was autoclaved. A standardized dilution of V. cholerae O1 Inaba and of V. cholerae O1 Ogawa was inoculated in three aliquots of 100ml of these different water samples and

  2. Água virtual, escassez e gestão: o Brasil como grande "exportador" de água Virtual water, scarcity and management: Brazil as a large water exporter

    Directory of Open Access Journals (Sweden)

    Roberto Luiz do Carmo

    2007-12-01

    Full Text Available Recentemente, a valoração econômica da água tem sido discutida no cenário político brasileiro. Faz parte dessa discussão a incorporação da água nos produtos que circulam no mercado, especialmente através de commodities, utilizando o conceito de "água virtual". Este trabalho avalia o impacto da utilização do conceito de água virtual no cenário brasileiro, ao mesmo tempo em que discute as decorrências dessa exportação.The economic valuation of water has recently been discussed in the Brazilian political scenario. Incorporation of water in trade and commerce, especially in commodities is part of the proposed discussion , using the "virtual water" concept. In this article we search for the impact that the utilization of the "virtual water" concept may have in the Brazilian scenario and discuss, at the same time, the consequences of this exportation.

  3. APROVEITAMENTO, CAPTAÇÃO E (RE) USO DAS ÁGUAS PLUVIAIS NA ARQUITETURA

    OpenAIRE

    Melhores Trabalhos .

    2015-01-01

    O presente artigo tem a intenção de abordar a captação e o (re) uso das águas pluviais com ênfase na arquitetura sustentável, tendo como finalidade básica a conscientização do consumo exagerado de água potável. O aproveitamento da água da chuva é uma das alternativas principais para a redução da escassez da água, conservação dos lençóis freáticos e para a colaboração no sistema de drenagem urbana. Dessa forma, a pesquisa apresentará informações referentes às várias formas e sistemas de captaç...

  4. Aproveitamento de águas pluviais e o reúso de águas cinzas em edifícios residenciais de Brasília – parte 2: viabilidade técnica e econômica

    Directory of Open Access Journals (Sweden)

    Daniel Sant'Ana

    2013-12-01

    Full Text Available A viabilidade da implementação de sistemas deaproveitamento águas pluviais (AAP e de reúsode águas cinzas (RAC em edifícios residenciaisé analisada e os resultados são apresentadosem dois artigos. A primeira parte caracteriza osusos-finais do consumo doméstico de água deedificações residenciais de Brasília e identifica opotencial de redução no consumo de água promovidopor diferentes sistemas AAP e RAC. Asegunda parte aponta possíveis soluções paraadaptação predial das tipologias residenciais deBrasília e faz uma avaliação financeira dos custos-benefícios para diferentes tipos de sistemasAAP e RAC. Em geral, resultados indicam quea adaptação predial voltada ao aproveitamentode águas pluviais em demandas externas é umasolução simples e rentável. Em alguns casos,reservatórios inferiores de água fria podem sermodificados para servir como cisternas de águapluvial, reduzindo custos de capital. Adaptaçãopredial voltada ao aproveitamento de águaspluviais em demandas internas demonstrou seruma solução inviável devido aos altos custosde reforma. Por outro lado, sistemas de reúsode águas cinzas em demandas internas promovembenefícios financeiros significativos, o queacaba compensando os custos de reforma. Oreúso de águas cinzas em demandas externasdemonstrou ser uma alternativa inviável devidoaos altos custos do sistema e aos baixos benefíciosfinanceiros.

  5. Gene-gene interactions of IRF5, STAT4, IKZF1 and ETS1 in systemic lupus erythematosus.

    Science.gov (United States)

    Dang, J; Shan, S; Li, J; Zhao, H; Xin, Q; Liu, Y; Bian, X; Liu, Q

    2014-06-01

    Interferon (IFN) activation signaling and T helper 17 (Th17)-cell/B-cell regulation play a critical role in the pathogenesis of systemic lupus erythematosus (SLE). Several studies have provided convincing evidence that polymorphisms in IRF5, STAT4, IKZF1 and ETS1 from these pathways may be involved in SLE by affecting gene expression or epistasis. We analyzed the genetic interaction in known SLE susceptibility loci from the four genes in northern Han Chinese. A total of 946 northern Han Chinese participated in this study (370 unrelated SLE patients and 576 healthy controls). Subjects underwent genotyping for the single-nucleotide polymorphisms (SNPs) rs2004640 in IRF5, rs7574865 in STAT4, rs4917014 in IKZF1 and rs1128334 in ETS1 by use of a TaqMan SNP genotyping assay and direct sequencing. Gene-gene interaction analysis involved direct counting, multifactor dimensionality reduction (MDR) and linear regression analysis. SLE patients and controls differed in allele frequencies of rs7574865, rs1128334 (P < 0.001) and rs4917014 (P < 0.01). Direct counting revealed that the frequency of risk homozygote combinations was higher for SLE patients than controls (P < 0.01). Furthermore, 2-, 3- and 4-way gene-gene epistasis in SLE was confirmed by parametric methods and MDR analysis. Gene expression analysis partially supported the findings. Our study confirmed the association of the IFN pathway or Th17/B-cells and the pathogenesis of SLE, and gene-gene interaction in this pathway may increase the risk of SLE. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  6. Tendências no processamento de frangos de corte: uso racional da água

    Directory of Open Access Journals (Sweden)

    Ricardo Lacava Bailone

    Full Text Available RESUMO Atualmente o uso racional da água vem sendo tema de discussão perante órgãos governamentais e não governamentais ao redor do mundo. Seguindo essa tendência, leis cada vez mais severas devem ser implantadas em relação ao uso desse bem natural. Em um frigorífico de frango, o uso da água ocorre de forma generalizada ao longo de todo o processo industrial. Nesse contexto, o atual estudo teve o objetivo de comparar o consumo de água em um frigorífico brasileiro de frangos de corte (grande porte com modelos já propostos e utilizados em outros países com o intuito de identificar soluções para mitigar perdas provenientes de sua má gestão. Concluiu-se que alterações em alguns pontos-chave do processamento possibilitariam à indústria o uso mais sustentável da água, como substituição da eletronarcose por insensibilização a gás, diminuição da dimensão do tanque de escaldagem ou mudança no processo, substituição do pré-resfriamento por imersão em água por pré-resfriamento a ar, redução da renovação de água nos tanques resfriadores, alterações no sistema de geração do frio em ambientes climatizados, assim como o reúso total ou parcial da água.

  7. Tendências no processamento de frangos de corte: uso racional da água

    Directory of Open Access Journals (Sweden)

    Ricardo Lacava Bailone

    2016-10-01

    Full Text Available RESUMO Atualmente o uso racional da água vem sendo tema de discussão perante órgãos governamentais e não governamentais ao redor do mundo. Seguindo essa tendência, leis cada vez mais severas devem ser implantadas em relação ao uso desse bem natural. Em um frigorífico de frango, o uso da água ocorre de forma generalizada ao longo de todo o processo industrial. Nesse contexto, o atual estudo teve o objetivo de comparar o consumo de água em um frigorífico brasileiro de frangos de corte (grande porte com modelos já propostos e utilizados em outros países com o intuito de identificar soluções para mitigar perdas provenientes de sua má gestão. Concluiu-se que alterações em alguns pontos-chave do processamento possibilitariam à indústria o uso mais sustentável da água, como substituição da eletronarcose por insensibilização a gás, diminuição da dimensão do tanque de escaldagem ou mudança no processo, substituição do pré-resfriamento por imersão em água por pré-resfriamento a ar, redução da renovação de água nos tanques resfriadores, alterações no sistema de geração do frio em ambientes climatizados, assim como o reúso total ou parcial da água.

  8. Bactérias do gênero Aeromonas em água de matadouro bovino Aeromonas bacteria in beef slaughtering water

    Directory of Open Access Journals (Sweden)

    O.D. Rossi Jr

    2000-10-01

    Full Text Available Verificou-se a ocorrência de bactérias do gênero Aeromonas em amostras de água (abastecimento/residuária obtidas em matadouro bovino. Analisaram-se a água utilizada nas dependências internas, a água dos currais, utilizada na dessedentação, pré-higienização e tranqüilização dos animais e a água residuária da lavagem das carcaças. Das 30 amostras representativas de cada tipo, bactérias do gênero Aeromonas foram isoladas em 10 (33,3% amostras da água dos currais e em 10 (33,3% amostras da água residuária da lavagem de carcaças. Nenhuma das amostras da água tratada de abastecimento das instalações revelou-se positiva no isolamento. As espécies isoladas foram Aeromonas hydrophila em duas (2,2% e Aeromonas caviae em 19 (21,1% amostras. Uma cepa considerada atípica foi isolada da água dos currais. Os resultados evidenciaram que a água dos currais pode ser uma importante fonte de contaminação, principalmente para a pele, e através dela as Aeromonas sp. podem chegar à sala de matança.The aim of this work was to verify the occurrence of Aeromonas bacteria in samples of water (supply and residual collected at beef slaughterhouse. Water used at the internal facilities, water from corrals, drinking water, water for pre-hygiene and tranquilization of the animals and residual water from carcasses’ wash, were analyzed. From those 30 samples of each type, Aeromonas bacteria were isolated in 10 (33.3% samples of corral water and in 10 (33.3% samples of residual water from carcasses’ wash. None of the facilities treated water supply samples showed positive in isolation. The isolated species were Aeromonas hydrophila in two (2.2% and Aeromonas caviae in 19 (21.1% of the samples. One non-typical strain was isolated from corral water. The results demonstrated that corral water may be an important contamination source, mainly to the hide and, through it, Aeromonas sp. can reach the slaughter room.

  9. Preservation of genes involved in sterol metabolism in cholesterol auxotrophs: facts and hypotheses.

    Directory of Open Access Journals (Sweden)

    Giovanna Vinci

    Full Text Available BACKGROUND: It is known that primary sequences of enzymes involved in sterol biosynthesis are well conserved in organisms that produce sterols de novo. However, we provide evidence for a preservation of the corresponding genes in two animals unable to synthesize cholesterol (auxotrophs: Drosophila melanogaster and Caenorhabditis elegans. PRINCIPAL FINDINGS: We have been able to detect bona fide orthologs of several ERG genes in both organisms using a series of complementary approaches. We have detected strong sequence divergence between the orthologs of the nematode and of the fruitfly; they are also very divergent with respect to the orthologs in organisms able to synthesize sterols de novo (prototrophs. Interestingly, the orthologs in both the nematode and the fruitfly are still under selective pressure. It is possible that these genes, which are not involved in cholesterol synthesis anymore, have been recruited to perform different new functions. We propose a more parsimonious way to explain their accelerated evolution and subsequent stabilization. The products of ERG genes in prototrophs might be involved in several biological roles, in addition to sterol synthesis. In the case of the nematode and the fruitfly, the relevant genes would have lost their ancestral function in cholesterogenesis but would have retained the other function(s, which keep them under pressure. CONCLUSIONS: By exploiting microarray data we have noticed a strong expressional correlation between the orthologs of ERG24 and ERG25 in D. melanogaster and genes encoding factors involved in intracellular protein trafficking and folding and with Start1 involved in ecdysteroid synthesis. These potential functional connections are worth being explored not only in Drosophila, but also in Caenorhabditis as well as in sterol prototrophs.

  10. Aproveitamento de águas pluviais e o reúso de águas cinzas em edifícios residenciais de Brasília – parte 2: viabilidade técnica e econômica

    OpenAIRE

    Sant'Ana, Daniel; Boeger, Louise; Monteiro, Lilian

    2013-01-01

    A viabilidade da implementação de sistemas deaproveitamento águas pluviais (AAP) e de reúsode águas cinzas (RAC) em edifícios residenciaisé analisada e os resultados são apresentadosem dois artigos. A primeira parte caracteriza osusos-finais do consumo doméstico de água deedificações residenciais de Brasília e identifica opotencial de redução no consumo de água promovidopor diferentes sistemas AAP e RAC. Asegunda parte aponta possíveis soluções paraadaptação predial das tipologias residenciai...

  11. Involvement of the VEP1 gene in vascular strand development in Arabidopsis thaliana.

    Science.gov (United States)

    Jun, Ji Hyung; Ha, Chan Man; Nam, Hong Gil

    2002-03-01

    A dominant mutant line characterized by abnormal leaf venation pattern was isolated from a transgenic Arabidopsis plant pool that was generated with Agrobacterium culture harboring an Arabidopsis antisense cDNA library. In the mutant line, the phenotype was due to antisense suppression of a gene we named VEP1 (Vein Patterning). The predicted amino acid sequence of the gene contained a motif related to the mammalian death domain that is found in the apoptotic machinery. Reduced expression of the VEP1 gene resulted in the reduced complexity of the venation pattern of the cotyledons and foliar leaves, which was mainly due to the reduced number of the minor veins and their incomplete connection. The analysis of mutant embryos indicated that the phenotype was originated, at least in part, from a defect in the procambium patterning. In the mutant, the stem and root were thinner than those in wild type. This phenotype was associated with reduced vascular development. The promoter activity of the VEP1 gene was detected preferentially in the vascular regions. We propose that the death domain-containing protein VEP1 functions as a positive element required for vascular strand development in Arabidopsis thaliana.

  12. Prediction of novel target genes and pathways involved in bevacizumab-resistant colorectal cancer

    Science.gov (United States)

    Makondi, Precious Takondwa; Lee, Chia-Hwa; Huang, Chien-Yu; Chu, Chi-Ming; Chang, Yu-Jia

    2018-01-01

    Bevacizumab combined with cytotoxic chemotherapy is the backbone of metastatic colorectal cancer (mCRC) therapy; however, its treatment efficacy is hampered by therapeutic resistance. Therefore, understanding the mechanisms underlying bevacizumab resistance is crucial to increasing the therapeutic efficacy of bevacizumab. The Gene Expression Omnibus (GEO) database (dataset, GSE86525) was used to identify the key genes and pathways involved in bevacizumab-resistant mCRC. The GEO2R web tool was used to identify differentially expressed genes (DEGs). Functional and pathway enrichment analyses of the DEGs were performed using the Database for Annotation, Visualization, and Integrated Discovery(DAVID). Protein–protein interaction (PPI) networks were established using the Search Tool for the Retrieval of Interacting Genes/Proteins database(STRING) and visualized using Cytoscape software. A total of 124 DEGs were obtained, 57 of which upregulated and 67 were downregulated. PPI network analysis showed that seven upregulated genes and nine downregulated genes exhibited high PPI degrees. In the functional enrichment, the DEGs were mainly enriched in negative regulation of phosphate metabolic process and positive regulation of cell cycle process gene ontologies (GOs); the enriched pathways were the phosphoinositide 3-kinase-serine/threonine kinase signaling pathway, bladder cancer, and microRNAs in cancer. Cyclin-dependent kinase inhibitor 1A(CDKN1A), toll-like receptor 4 (TLR4), CD19 molecule (CD19), breast cancer 1, early onset (BRCA1), platelet-derived growth factor subunit A (PDGFA), and matrix metallopeptidase 1 (MMP1) were the DEGs involved in the pathways and the PPIs. The clinical validation of the DEGs in mCRC (TNM clinical stages 3 and 4) revealed that high PDGFA expression levels were associated with poor overall survival, whereas high BRCA1 and MMP1 expression levels were associated with favorable progress free survival(PFS). The identified genes and pathways

  13. Prediction of novel target genes and pathways involved in bevacizumab-resistant colorectal cancer.

    Directory of Open Access Journals (Sweden)

    Precious Takondwa Makondi

    Full Text Available Bevacizumab combined with cytotoxic chemotherapy is the backbone of metastatic colorectal cancer (mCRC therapy; however, its treatment efficacy is hampered by therapeutic resistance. Therefore, understanding the mechanisms underlying bevacizumab resistance is crucial to increasing the therapeutic efficacy of bevacizumab. The Gene Expression Omnibus (GEO database (dataset, GSE86525 was used to identify the key genes and pathways involved in bevacizumab-resistant mCRC. The GEO2R web tool was used to identify differentially expressed genes (DEGs. Functional and pathway enrichment analyses of the DEGs were performed using the Database for Annotation, Visualization, and Integrated Discovery(DAVID. Protein-protein interaction (PPI networks were established using the Search Tool for the Retrieval of Interacting Genes/Proteins database(STRING and visualized using Cytoscape software. A total of 124 DEGs were obtained, 57 of which upregulated and 67 were downregulated. PPI network analysis showed that seven upregulated genes and nine downregulated genes exhibited high PPI degrees. In the functional enrichment, the DEGs were mainly enriched in negative regulation of phosphate metabolic process and positive regulation of cell cycle process gene ontologies (GOs; the enriched pathways were the phosphoinositide 3-kinase-serine/threonine kinase signaling pathway, bladder cancer, and microRNAs in cancer. Cyclin-dependent kinase inhibitor 1A(CDKN1A, toll-like receptor 4 (TLR4, CD19 molecule (CD19, breast cancer 1, early onset (BRCA1, platelet-derived growth factor subunit A (PDGFA, and matrix metallopeptidase 1 (MMP1 were the DEGs involved in the pathways and the PPIs. The clinical validation of the DEGs in mCRC (TNM clinical stages 3 and 4 revealed that high PDGFA expression levels were associated with poor overall survival, whereas high BRCA1 and MMP1 expression levels were associated with favorable progress free survival(PFS. The identified genes and pathways

  14. Cokrigagem colocada aplicada ao mapeamento do nível de água subterrânea

    OpenAIRE

    Boezio,Maria Noel Morales; Costa,João Felipe C. L.; Koppe,Jair C.

    2006-01-01

    A mineração subterrânea pode afetar o ciclo hidrológico, especialmente a circulação da água de infiltração de uma dada região. Em particular, o nível de água pode ser modificado, refletindo-se no meio ambiente circundante. Em programas de monitoramento de água subterrânea, o mapeamento do nível de água é parte fundamental. O mesmo ajuda na determinação da direção de fluxo da água subterrânea, identificação de zonas de recarga e avaliação da dispersão de contaminantes. A posição do nível de ág...

  15. Estudo comparativo do resfriamento de laranja valência com ar forçado e com água

    Directory of Open Access Journals (Sweden)

    Teruel Bárbara

    2003-01-01

    Full Text Available Este trabalho apresenta um estudo para comparar o resfriamento rápido de laranja Valência (Citrus sinensis O., com ar forçado e com água gelada. O sistema de resfriamento rápido com ar forçado operou com um fluxo de ar de 1.933m³/h (3 l/s por kg de produto resfriado, a uma temperatura de 1ºC e UR=88,4 ± 2,0%, com uma velocidade do ar em torno de 1m/s. Nos experimentos com água gelada foi utilizado um sistema de imersão, com uma capacidade de 0,23m³ de água a uma temperatura de aproximadamente 1ºC. Foi determinando o tempo meio e de sete oitavos do resfriamento, partindo das leituras de temperatura, perfazendo-se duas repetições em cada experimento. Os resultados mostraram que, o resfriamento do leito de frutas com ar forçado não é homogêneo, obtendo-se um tempo de sete oitavos do resfriamento que variaram de 107min a 170min, dependendo da posição do fruto no interior da embalagem. Já no sistema com água gelada, o resfriamento acontece uniformemente em todo em todo o leito de frutos, sendo o tempo médio de resfriamento de 57min.

  16. IMPLANTAÇÃO DE SISTEMA DE CONSERVAÇÃO E REUSO DE ÁGUA EM TERMINAL PORTUÁRIO DE SALVADOR

    OpenAIRE

    Moura, Adriana Correia Calmon; Linhares, Camila Chamusca; Grillo, Caroline Gualberto; Viana, Clarissa Meira; Costa, Fransival Pereira

    2018-01-01

    O Terminal de Contêineres Salvador (TECON), dentre diversas atividades realizadas no empreendimento, a água é um insumo de grande importância haja vista o volume mensal médio demandado pela TECON, atingindo o valor de 1.627 m³, ou seja, 1.627.000 (um milhão e seiscentos e vinte sete mil) litros de água potável por mês. O projeto de conservação e reuso de água do Tecon Salvador contou com a instalação de hidrômetros individualizados por edificação e pontos de consumo, implantação de sistema de...

  17. A genome-wide search for genes involved in the radiation-induced gastroschisis

    International Nuclear Information System (INIS)

    Hillebrandt, S.; Streffer, C.

    1997-01-01

    Whole genome linkage analysis of gastroschisis (abdominal wall defect) using geno-typing with micro-satellites of affected BC1 mice [(HLGxC57BL/6J)xHLG] was performed. The HLG inbred strain shows an increased risk in gastroschisis after irradiation of embryos in the 1-cell stage. Previous studies demonstrated, that gastroschisis is a poly-genic trait with a recessive mode of inheritance. Since a recessive inheritance of gastroschisis is assumed, the involved genes must be linked to markers showing a high level of homozygosity in the affected animals. For marker loci on the chromosome 13 and 19 a significantly increased number of homozygotes has been found in mice with gastroschisis comparing to mice without this malformation. The linkage analysis performed by us allowed determining intervals likely to contain genes related to gastroschisis on these two chromosomes. The highest lod score value has been found for the marker locus D19MIT27 very close to Pax2 (lod score=1.23; p=0.017). For the marker D13MIT99 a lod score of 0.85 (p=0.047) was calculated. However, markers more close to the homeo-box gene Msx-2 on the chromosome 13 show lower lod score values than D13MIT99, suggesting that this homeo-box gene is probably not involved in gastroschisis. According to the classification of results of the linkage analysis of complex traits described by Lander and Kruglyak (1995), our data provide a suggestive evidence for the involvement of the analyzed intervals on the chromosomes 19 and 13 to gastroschisis. Further studies are necessary to prove this linkage. (authors)

  18. Diferenciação genética entre populações de Pseudoplatystoma corruscans (Agassiz, 1829 (Osteichthyes, Pimelodidae isoladas pelos saltos de Guaíra do rio Paraná Genetic differentiation among populations of Pseudoplatystoma corruscans (Agassiz, 1829 (Osteichthyes, Pimelodidae isolated by the Guaíra Falls in the Paraná River

    Directory of Open Access Journals (Sweden)

    Sônia Maria Alves Pinto Prioli

    2002-05-01

    Full Text Available Os saltos de Guaíra, também denominados Sete Quedas, constituíam até 1982 uma barreira para a dispersão de peixes migradores. Este trabalho teve por objetivo verificar se populações de Pseudoplatystoma corruscans (Osteichthyes, Pimelodidae eram isoladas pelos saltos de Guaíra. Amostras provenientes da planície de inundação do alto rio Paraná (PL, do reservatório Itaipu (IT e de jusante de Yacyretá (YA, foram comparadas por RAPD. O FST de Lynch foi significativo entre PL e IT (0,090 e PL e YA (0,112. O fluxo gênico estimado (Nm variou de 2,0 entre PL e YA a 8,1 entre IT e YA, e a distância genética de Nei de D = 0,0638 entre PL e YA a D = 0,0174 entre IT e YA. Estes resultados indicam existência de diferenciação genética e que, possivelmente, Sete Quedas isolavam reprodutivamente as populações. Sugerem, também, a possibilidade de áreas diferentes de desova, impedindo parcialmente a homogeneização genética das populações IT e PL.Until 1982, the Guaíra Falls, also named the Seven Falls, constituted a barrier to the dispersion of migratory fish in the Paraná River. The objective of this work was to verify if populations of Pseudoplatystoma corruscans (Osteichthyes, Pimelodidae were isolated by the Guaíra Falls. Samples from the Upper Paraná River floodplain (PL, the Itaipu reservoir (IT, and downstream Yacyretá reservoir (YA were compared by RAPD markers. Lynch's FST was significant between PL and IT (0.090, and PL and YA (0.112. Estimated gene flow (Nm varied from 2.0 between PL and YA to 8.1 between IT and YA. Nei's genetic distance varied from D = 0.0638 between PL and YA to D = 0.0174 between IT and YA. These results indicate the existence of genetic differentiation and that, possibly, the Guaíra Falls isolated the populations reproductively. They also suggest the possibility of different spawning areas, partially avoiding the genetic homogenization of the IT and PL populations.

  19. Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata

    Directory of Open Access Journals (Sweden)

    Hien P. Nguyen

    2017-12-01

    Full Text Available The establishment of a root nodule symbiosis between a leguminous plant and a rhizobium requires complex molecular interactions between the two partners. Compatible interactions lead to the formation of nitrogen-fixing nodules, however, some legumes exhibit incompatibility with specific rhizobial strains and restrict nodulation by the strains. Bradyrhizobium elkanii USDA61 is incompatible with mung bean (Vigna radiata cv. KPS1 and soybean cultivars carrying the Rj4 allele. Here, we explored genetic loci in USDA61 that determine incompatibility with V. radiata KPS1. We identified five novel B. elkanii genes that contribute to this incompatibility. Four of these genes also control incompatibility with soybean cultivars carrying the Rj4 allele, suggesting that a common mechanism underlies nodulation restriction in both legumes. The fifth gene encodes a hypothetical protein that contains a tts box in its promoter region. The tts box is conserved in genes encoding the type III secretion system (T3SS, which is known for its delivery of virulence effectors by pathogenic bacteria. These findings revealed both common and unique genes that are involved in the incompatibility of B. elkanii with mung bean and soybean. Of particular interest is the novel T3SS-related gene, which causes incompatibility specifically with mung bean cv. KPS1.

  20. Carbon: Nitrogen Interaction Regulates Expression of Genes Involved in N-Uptake and Assimilation in Brassica juncea L.

    Science.gov (United States)

    Goel, Parul; Bhuria, Monika; Kaushal, Mamta

    2016-01-01

    In plants, several cellular and metabolic pathways interact with each other to regulate processes that are vital for their growth and development. Carbon (C) and Nitrogen (N) are two main nutrients for plants and coordination of C and N pathways is an important factor for maintaining plant growth and development. In the present work, influence of nitrogen and sucrose (C source) on growth parameters and expression of genes involved in nitrogen transport and assimilatory pathways was studied in B. juncea seedlings. For this, B. juncea seedlings were treated with four combinations of C and N source viz., N source alone (-Suc+N), C source alone (+Suc-N), with N and C source (+Suc+N) or without N and C source (-Suc-N). Cotyledon size and shoot length were found to be increased in seedlings, when nitrogen alone was present in the medium. Distinct expression pattern of genes in both, root and shoot tissues was observed in response to exogenously supplied N and C. The presence or depletion of nitrogen alone in the medium leads to severe up- or down-regulation of key genes involved in N-uptake and transport (BjNRT1.1, BjNRT1.8) in root tissue and genes involved in nitrate reduction (BjNR1 and BjNR2) in shoot tissue. Moreover, expression of several genes, like BjAMT1.2, BjAMT2 and BjPK in root and two genes BjAMT2 and BjGS1.1 in shoot were found to be regulated only when C source was present in the medium. Majority of genes were found to respond in root and shoot tissues, when both C and N source were present in the medium, thus reflecting their importance as a signal in regulating expression of genes involved in N-uptake and assimilation. The present work provides insight into the regulation of genes of N-uptake and assimilatory pathway in B. juncea by interaction of both carbon and nitrogen. PMID:27637072

  1. Carbon: Nitrogen Interaction Regulates Expression of Genes Involved in N-Uptake and Assimilation in Brassica juncea L.

    Directory of Open Access Journals (Sweden)

    Parul Goel

    Full Text Available In plants, several cellular and metabolic pathways interact with each other to regulate processes that are vital for their growth and development. Carbon (C and Nitrogen (N are two main nutrients for plants and coordination of C and N pathways is an important factor for maintaining plant growth and development. In the present work, influence of nitrogen and sucrose (C source on growth parameters and expression of genes involved in nitrogen transport and assimilatory pathways was studied in B. juncea seedlings. For this, B. juncea seedlings were treated with four combinations of C and N source viz., N source alone (-Suc+N, C source alone (+Suc-N, with N and C source (+Suc+N or without N and C source (-Suc-N. Cotyledon size and shoot length were found to be increased in seedlings, when nitrogen alone was present in the medium. Distinct expression pattern of genes in both, root and shoot tissues was observed in response to exogenously supplied N and C. The presence or depletion of nitrogen alone in the medium leads to severe up- or down-regulation of key genes involved in N-uptake and transport (BjNRT1.1, BjNRT1.8 in root tissue and genes involved in nitrate reduction (BjNR1 and BjNR2 in shoot tissue. Moreover, expression of several genes, like BjAMT1.2, BjAMT2 and BjPK in root and two genes BjAMT2 and BjGS1.1 in shoot were found to be regulated only when C source was present in the medium. Majority of genes were found to respond in root and shoot tissues, when both C and N source were present in the medium, thus reflecting their importance as a signal in regulating expression of genes involved in N-uptake and assimilation. The present work provides insight into the regulation of genes of N-uptake and assimilatory pathway in B. juncea by interaction of both carbon and nitrogen.

  2. Characterization of Genes Encoding Key Enzymes Involved in Anthocyanin Metabolism of Kiwifruit during Storage Period.

    Science.gov (United States)

    Li, Boqiang; Xia, Yongxiu; Wang, Yuying; Qin, Guozheng; Tian, Shiping

    2017-01-01

    'Hongyang' is a red fleshed kiwifruit with high anthocyanin content. In this study, we mainly investigated effects of different temperatures (25 and 0°C) on anthocyanin biosynthesis in harvested kiwifruit, and characterized the genes encoding key enzymes involved in anthocyanin metabolism, as well as evaluated the mode of the action, by which low temperature regulates anthocyanin accumulation in 'Hongyang' kiwifruit during storage period. The results showed that low temperature could effectively enhance the anthocyanin accumulation of kiwifruit in the end of storage period (90 days), which related to the increase in mRNA levels of ANS1, ANS2, DRF1, DRF2 , and UGFT2 . Moreover, the transcript abundance of MYBA1-1 and MYB5-1 , the genes encoding an important component of MYB-bHLH-WD40 (MBW) complex, was up-regulated, possibly contributing to the induction of specific anthocyanin biosynthesis genes under the low temperature. To further investigate the roles of AcMYB5-1/5-2/A1-1 in regulation of anthocyanin biosynthesis, genes encoding the three transcription factors were transiently transformed in Nicotiana benthamiana leaves. Overexpression of AcMYB5-1/5-2/A1-1 activated the gene expression of NtANS and NtDFR in tobacco. Our results suggested that low temperature storage could stimulate the anthocyanin accumulation in harvested kiwifruit via regulating several structural and regulatory genes involved in anthocyanin biosynthesis.

  3. Molecular characterization of genes encoding leucoanthocyanidin reductase involved in proanthocyanidin biosynthesis in apple

    Directory of Open Access Journals (Sweden)

    Yuepeng eHan

    2015-04-01

    Full Text Available Proanthocyanidins (PAs are the major component of phenolics in apple, but mechanisms involved in PA biosynthesis remain unclear. Here, the relationship between the PA biosynthesis and the expression of genes encoding leucoanthocyanidin reductase (LAR and anthocyanidin reductase (ANR was investigated in fruit skin of one apple cultivar and three crabapples. Transcript levels of LAR1 and ANR2 genes were significantly correlated with the contents of catechin and epicatechin, respectively, which suggests their active roles in PA synthesis. Surprisingly, transcript levels for both LAR1 and LAR2 genes were almost undetectable in two crabapples that accumulated both flavan-3-ols and PAs. This contradicts the previous finding that LAR1 gene is a strong candidate regulating the accumulation of metabolites such as epicatechin and PAs in apple. Ectopic expression of apple MdLAR1 gene in tobacco suppresses expression of the late genes in anthocyanin biosynthetic pathway, resulting in loss of anthocyanin in flowers. Interestingly, a decrease in PA biosynthesis was also observed in flowers of transgenic tobacco plants overexpressing the MdLAR1 gene, which could be attributed to decreased expression of both the NtANR1 and NtANR2 genes. Our study not only confirms the in vivo function of apple LAR1 gene, but it is also helpful for understanding the mechanism of PA biosynthesis.

  4. Evaluation of common variants in 16 genes involved in the regulation of neurotransmitter release in ADHD.

    Science.gov (United States)

    Sánchez-Mora, Cristina; Cormand, Bru; Ramos-Quiroga, Josep Antoni; Hervás, Amaia; Bosch, Rosa; Palomar, Glòria; Nogueira, Mariana; Gómez-Barros, Núria; Richarte, Vanesa; Corrales, Montse; Garcia-Martinez, Iris; Corominas, Roser; Guijarro, Silvina; Bigorra, Aitana; Bayés, Mònica; Casas, Miguel; Ribasés, Marta

    2013-06-01

    Attention-deficit hyperactivity disorder (ADHD) is a neurobehavioral disorder characterized by inappropriate difficulties to sustain attention, control impulses and modulate activity level. Although ADHD is one of the most prevalent childhood psychiatric disorders, it also persists into adulthood in around 30-50% of the cases. Based on the effect of psychostimulants used in the pharmacological treatment of ADHD, dysfunctions in neuroplasticity mechanisms and synapses have been postulated to be involved in the pathophysiology of ADHD. With this background, we evaluated, both in childhood and adulthood ADHD, the role of several genes involved in the control of neurotransmitter release through synaptic vesicle docking, fusion and recycling processes by means of a population-based association study. We analyzed single nucleotide polymorphisms across 16 genes in a clinical sample of 950 ADHD patients (506 adults and 444 children) and 905 controls. Single and multiple-marker analyses identified several significant associations after correcting for multiple testing with a false discovery rate (FDR) of 15%: (i) the SYT2 gene was strongly associated with both adulthood and childhood ADHD (p=0.001, OR=1.49 (1.18-1.89) and p=0.007, OR=1.37 (1.09-1.72), respectively) and (ii) STX1A was found associated with ADHD only in adults (p=0.0041; OR=1.28 (1.08-1.51)). These data provide preliminary evidence for the involvement of genes that participate in the control of neurotransmitter release in the genetic predisposition to ADHD through a gene-system association study. Further follow-up studies in larger cohorts and deep-sequencing of the associated genomic regions are required to identify sequence variants directly involved in ADHD. Copyright © 2012 Elsevier B.V. and ECNP. All rights reserved.

  5. Tris(1,3-dichloro-2-propyl) phosphate perturbs the expression of genes involved in immune response and lipid and steroid metabolism in chicken embryos

    International Nuclear Information System (INIS)

    Farhat, Amani; Buick, Julie K.; Williams, Andrew; Yauk, Carole L.; O'Brien, Jason M.; Crump, Doug; Williams, Kim L.; Chiu, Suzanne; Kennedy, Sean W.

    2014-01-01

    We previously demonstrated that in ovo exposure to the flame retardant tris(1,3-dichloro-2-propyl) phosphate (TDCPP) decreased plasma thyroxine levels, reduced growth parameters, and decreased gallbladder size in chicken embryos. In the current study DNA microarrays were used to evaluate global mRNA expression in liver tissue of male chicken embryos that exhibited the above mentioned effects. Injected doses were dimethyl sulfoxide vehicle control, 7.6 or 45 μg TDCPP/g egg. TDCPP caused significant changes in the expression of five genes at the low dose and 47 genes at the high dose (False Discovery Rate p ≤ 0.1, fold change ≥ 1.5). The gene expression analysis suggested a compromised immune function, a state of cholestatic liver/biliary fibrosis, and disrupted lipid and steroid metabolism. Circulating bile acid levels were elevated, which is an indication of liver dysfunction, and plasma cholesterol levels were reduced; however, hepatic bile acid and cholesterol levels were unaltered. Interactome analyses identified apolipoprotein E, hepatocyte nuclear factor 4 alpha, and peroxisome proliferator-activated receptor alpha as key regulatory molecules involved in the effects of TDCPP. Our results demonstrate a targeted effect of TDCPP toxicity on lipid metabolism, including cholesterol, that helps explain the aforementioned phenotypic effects, as chicken embryos are highly dependent on yolk lipids for growth and maintenance throughout development. Finally, our results are in concordance with the literature that describes TDCPP as a cancer-causing agent, since the majority of dysregulated genes were involved in cancer pathways. - Highlights: • TDCPP dysregulates genes involved in immune function and lipid metabolism. • A targeted effect of TDCPP toxicity on cholesterol metabolism is apparent. • A state of cholestatic liver fibrosis is suggested by the expression profile. • Elevated plasma bile acids suggest that TDCPP causes liver dysfunction

  6. Tris(1,3-dichloro-2-propyl) phosphate perturbs the expression of genes involved in immune response and lipid and steroid metabolism in chicken embryos

    Energy Technology Data Exchange (ETDEWEB)

    Farhat, Amani [Department of Biology, University of Ottawa, Ottawa, ON K1N 6N5 (Canada); National Wildlife Research Centre, Environment Canada, Ottawa, ON K1A 0H3 (Canada); Buick, Julie K.; Williams, Andrew; Yauk, Carole L.; O' Brien, Jason M. [Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON K1A 0K9 (Canada); Crump, Doug; Williams, Kim L.; Chiu, Suzanne [National Wildlife Research Centre, Environment Canada, Ottawa, ON K1A 0H3 (Canada); Kennedy, Sean W., E-mail: sean.kennedy@ec.gc.ca [Department of Biology, University of Ottawa, Ottawa, ON K1N 6N5 (Canada); National Wildlife Research Centre, Environment Canada, Ottawa, ON K1A 0H3 (Canada)

    2014-03-01

    We previously demonstrated that in ovo exposure to the flame retardant tris(1,3-dichloro-2-propyl) phosphate (TDCPP) decreased plasma thyroxine levels, reduced growth parameters, and decreased gallbladder size in chicken embryos. In the current study DNA microarrays were used to evaluate global mRNA expression in liver tissue of male chicken embryos that exhibited the above mentioned effects. Injected doses were dimethyl sulfoxide vehicle control, 7.6 or 45 μg TDCPP/g egg. TDCPP caused significant changes in the expression of five genes at the low dose and 47 genes at the high dose (False Discovery Rate p ≤ 0.1, fold change ≥ 1.5). The gene expression analysis suggested a compromised immune function, a state of cholestatic liver/biliary fibrosis, and disrupted lipid and steroid metabolism. Circulating bile acid levels were elevated, which is an indication of liver dysfunction, and plasma cholesterol levels were reduced; however, hepatic bile acid and cholesterol levels were unaltered. Interactome analyses identified apolipoprotein E, hepatocyte nuclear factor 4 alpha, and peroxisome proliferator-activated receptor alpha as key regulatory molecules involved in the effects of TDCPP. Our results demonstrate a targeted effect of TDCPP toxicity on lipid metabolism, including cholesterol, that helps explain the aforementioned phenotypic effects, as chicken embryos are highly dependent on yolk lipids for growth and maintenance throughout development. Finally, our results are in concordance with the literature that describes TDCPP as a cancer-causing agent, since the majority of dysregulated genes were involved in cancer pathways. - Highlights: • TDCPP dysregulates genes involved in immune function and lipid metabolism. • A targeted effect of TDCPP toxicity on cholesterol metabolism is apparent. • A state of cholestatic liver fibrosis is suggested by the expression profile. • Elevated plasma bile acids suggest that TDCPP causes liver dysfunction.

  7. Examination of Signatures of Recent Positive Selection on Genes Involved in Human Sialic Acid Biology.

    Science.gov (United States)

    Moon, Jiyun M; Aronoff, David M; Capra, John A; Abbot, Patrick; Rokas, Antonis

    2018-03-28

    Sialic acids are nine carbon sugars ubiquitously found on the surfaces of vertebrate cells and are involved in various immune response-related processes. In humans, at least 58 genes spanning diverse functions, from biosynthesis and activation to recycling and degradation, are involved in sialic acid biology. Because of their role in immunity, sialic acid biology genes have been hypothesized to exhibit elevated rates of evolutionary change. Consistent with this hypothesis, several genes involved in sialic acid biology have experienced higher rates of non-synonymous substitutions in the human lineage than their counterparts in other great apes, perhaps in response to ancient pathogens that infected hominins millions of years ago (paleopathogens). To test whether sialic acid biology genes have also experienced more recent positive selection during the evolution of the modern human lineage, reflecting adaptation to contemporary cosmopolitan or geographically-restricted pathogens, we examined whether their protein-coding regions showed evidence of recent hard and soft selective sweeps. This examination involved the calculation of four measures that quantify changes in allele frequency spectra, extent of population differentiation, and haplotype homozygosity caused by recent hard and soft selective sweeps for 55 sialic acid biology genes using publicly available whole genome sequencing data from 1,668 humans from three ethnic groups. To disentangle evidence for selection from confounding demographic effects, we compared the observed patterns in sialic acid biology genes to simulated sequences of the same length under a model of neutral evolution that takes into account human demographic history. We found that the patterns of genetic variation of most sialic acid biology genes did not significantly deviate from neutral expectations and were not significantly different among genes belonging to different functional categories. Those few sialic acid biology genes that

  8. Plant Genes Involved in Symbiotic Sinal Perception/Signal Transduction

    DEFF Research Database (Denmark)

    Binder, A; Soyano, T; Hayashi, H

    2014-01-01

    to nodule primordia formation, and the infection thread initiation in the root hairs guiding bacteria towards dividing cortical cells. This chapter focuses on the plant genes involved in the recognition of the symbiotic signal produced by rhizobia, and the downstream genes, which are part of a complex...... symbiotic signalling pathway that leads to the generation of calcium spiking in the nuclear regions and activation of transcription factors controlling symbiotic genes induction...

  9. Transcriptome analysis identifies genes involved in ethanol response of Saccharomyces cerevisiae in Agave tequilana juice.

    Science.gov (United States)

    Ramirez-Córdova, Jesús; Drnevich, Jenny; Madrigal-Pulido, Jaime Alberto; Arrizon, Javier; Allen, Kirk; Martínez-Velázquez, Moisés; Alvarez-Maya, Ikuri

    2012-08-01

    During ethanol fermentation, yeast cells are exposed to stress due to the accumulation of ethanol, cell growth is altered and the output of the target product is reduced. For Agave beverages, like tequila, no reports have been published on the global gene expression under ethanol stress. In this work, we used microarray analysis to identify Saccharomyces cerevisiae genes involved in the ethanol response. Gene expression of a tequila yeast strain of S. cerevisiae (AR5) was explored by comparing global gene expression with that of laboratory strain S288C, both after ethanol exposure. Additionally, we used two different culture conditions, cells grown in Agave tequilana juice as a natural fermentation media or grown in yeast-extract peptone dextrose as artificial media. Of the 6368 S. cerevisiae genes in the microarray, 657 genes were identified that had different expression responses to ethanol stress due to strain and/or media. A cluster of 28 genes was found over-expressed specifically in the AR5 tequila strain that could be involved in the adaptation to tequila yeast fermentation, 14 of which are unknown such as yor343c, ylr162w, ygr182c, ymr265c, yer053c-a or ydr415c. These could be the most suitable genes for transforming tequila yeast to increase ethanol tolerance in the tequila fermentation process. Other genes involved in response to stress (RFC4, TSA1, MLH1, PAU3, RAD53) or transport (CYB2, TIP20, QCR9) were expressed in the same cluster. Unknown genes could be good candidates for the development of recombinant yeasts with ethanol tolerance for use in industrial tequila fermentation.

  10. Genome-wide identification of KANADI1 target genes.

    Directory of Open Access Journals (Sweden)

    Paz Merelo

    Full Text Available Plant organ development and polarity establishment is mediated by the action of several transcription factors. Among these, the KANADI (KAN subclade of the GARP protein family plays important roles in polarity-associated processes during embryo, shoot and root patterning. In this study, we have identified a set of potential direct target genes of KAN1 through a combination of chromatin immunoprecipitation/DNA sequencing (ChIP-Seq and genome-wide transcriptional profiling using tiling arrays. Target genes are over-represented for genes involved in the regulation of organ development as well as in the response to auxin. KAN1 affects directly the expression of several genes previously shown to be important in the establishment of polarity during lateral organ and vascular tissue development. We also show that KAN1 controls through its target genes auxin effects on organ development at different levels: transport and its regulation, and signaling. In addition, KAN1 regulates genes involved in the response to abscisic acid, jasmonic acid, brassinosteroids, ethylene, cytokinins and gibberellins. The role of KAN1 in organ polarity is antagonized by HD-ZIPIII transcription factors, including REVOLUTA (REV. A comparison of their target genes reveals that the REV/KAN1 module acts in organ patterning through opposite regulation of shared targets. Evidence of mutual repression between closely related family members is also shown.

  11. The space (reorganization in Guaíra after the end of “Sete Quedas”

    Directory of Open Access Journals (Sweden)

    Edson Belo Clemente de Souza

    2007-12-01

    Full Text Available This article analyses the space (re organization in themunicipality of Guaíra – PR after the formation of Itaipu Lakewith consequent immersions of the “Sete Quedas”. Due tothe socio-environmental impact caused by the flood, a newphase of development is going to be implemented in Guaírabased on the Directory Plans of 1980 and 1992, reorganizingthe geographic area of Guaíra in a corollary way.

  12. Determinação de contaminantes emergentes em mananciais de água bruta e na água para consumo humano do Distrito Federal

    OpenAIRE

    Santana, Joyce da Silva

    2013-01-01

    Contaminantes emergentes referem-se às substâncias químicas cujo interesse por estudos associados à ocorrência e efeitos no ambiente é recente. Ainda há uma escassez de dados relacionados à ocorrência destes contaminantes no Brasil, o que motivou a realização do presente trabalho. Foram selecionadas amostras de águas superficiais do Lago Paranoá e de dois mananciais de água do Distrito Federal (DF): Rio Descoberto e Córrego Cabeça de Veado. Também foram analisadas ...

  13. Regional assignment of seven genes on chromosome 1 of man by use of man-Chinese hamster somatic cell hybrids. I. Results obtained after hybridization of human cells carrying reciprocal translocations involving chromosome 1.

    Science.gov (United States)

    Jongsma, A P; Burgerhout, W G

    1977-01-01

    Regional localization studies of genes coding for human PGD, PPH1, PGM1, UGPP, GuK1, Pep-C, and FH, which have been assigned to chromosome 1, were performed with man-Chinese hamster somatic cell hybrids, Informative hybrids that retained fragments of the human chromosome 1 were produced by fusion of hamster cells with human cells carrying reciprocal translocations involving chromosome 1. Analysis of the hybrids that retained one of the translocation chromosomes or de novo rearrangements involving the human 1 revealed the following gene positions: PGD and PPH1 in 1pter leads to 1p32, PGM1 in 1p32 leads to 1p22, UGPP and GuK1 in 1q21 leads to 1q42, FH in 1qter leads to 1q42, and Pep-C probably in 1q42.

  14. Monitoramento de problemas com plantas aquáticas e caracterização da qualidade de água e sedimento na UHE Mogi-Guaçu Monitoring problems with aquatic plants and characterization of water and sediment quality at UHE Mogi-Guaçu

    Directory of Open Access Journals (Sweden)

    A.L. Cavenaghi

    2005-06-01

    Full Text Available Este trabalho foi realizado na UHE Mogi-Guaçu, pertencente à AES Tietê S.A. Levantamento de plantas aquáticas e amostragem de água e sedimento foram realizados em julho de 2001 (estação seca, novembro de 2001 (início da estação chuvosa e março de 2002 (final da estação chuvosa. Foram feitas 846 análises de água e 516 de sedimento, avaliando-se 47 características da água e 41 do sedimento. Espécies marginais e flutuantes foram as principais infestantes do reservatório, merecendo destaque as espécies Brachiaria subquadripara, Eichornia crassipes, Polygonum lapathifolium, Panicum rivulare, Salvinia auriculata e Pistia stratiotes. A turbidez e conseqüente baixa transmissão de luz pela coluna d'água impossibilitaram o desenvolvimento de plantas submersas. Teores de fósforo e nitrogênio encontrados tanto na água quanto no sedimento mostraram-se suficientes para suportar o crescimento de plantas aquáticas. A ocorrência de plantas marginais e flutuantes estava associada ao intenso processo de sedimentação observado no reservatório.This research was carried out at the Mogi Guaçu reservoir owned by AES Tiete. Assessment of aquatic plants and water and sediment sampling were carried out in July 2001 (dry season, November 2001 (start of rainy season and March 2002 (end of rainy season. A total of 846 water and 516 sediment analyses were performed, with 47 water and 41 sediment characteristics being evaluated. Marginal and floating species were the main weeds in the reservoir, the most outstanding being Brachiaria subquadripara, Eichornia crassipes, Polygonum lapathifolium, Panicum rivulare, Salvinia auriculata and Pistia stratiotes. Turbidity and low light transmission by water column made the growth of the submerged plants impossible. Phosphorus and nitrogen concentration both in the water and sediment was adequate to support aquatic plant growth. The occurrence of marginal and floating plants was associated with the intense

  15. BraLTP1, a lipid transfer protein gene involved in epicuticular wax deposition, cell proliferation and flower development in Brassica napus.

    Directory of Open Access Journals (Sweden)

    Fang Liu

    Full Text Available Plant non-specific lipid transfer proteins (nsLTPs constitute large multigene families that possess complex physiological functions, many of which remain unclear. This study isolated and characterized the function of a lipid transfer protein gene, BraLTP1 from Brassica rapa, in the important oilseed crops Brassica napus. BraLTP1 encodes a predicted secretory protein, in the little known VI Class of nsLTP families. Overexpression of BnaLTP1 in B. napus caused abnormal green coloration and reduced wax deposition on leaves and detailed wax analysis revealed 17-80% reduction in various major wax components, which resulted in significant water-loss relative to wild type. BnaLTP1 overexpressing leaves exhibited morphological disfiguration and abaxially curled leaf edges, and leaf cross-sections revealed cell overproliferation that was correlated to increased cytokinin levels (tZ, tZR, iP, and iPR in leaves and high expression of the cytokinin biosynthsis gene IPT3. BnaLTP1-overexpressing plants also displayed morphological disfiguration of flowers, with early-onset and elongated carpel development and outwardly curled stamen. This was consistent with altered expression of a a number of ABC model genes related to flower development. Together, these results suggest that BraLTP1 is a new nsLTP gene involved in wax production or deposition, with additional direct or indirect effects on cell division and flower development.

  16. Qualidade da água para consumo humano e concentração de fluoreto

    Directory of Open Access Journals (Sweden)

    Paulo Frazão

    2011-10-01

    Full Text Available O artigo visa analisar a concentração de fluoreto na água para consumo humano, considerando o balanço entre benefícios e riscos à saúde, e produzir subsídios para atualização da legislação brasileira. Estudos de revisão sistemática, documentos oficiais e dados meteorológicos foram examinados. As temperaturas nas capitais brasileiras indicam que o fluoreto deveria variar de 0,6 a 0,9 mg/L para prevenir cárie dentária. Concentração de fluoreto natural de 1,5 mg/L é tolerável para consumo no Brasil se não houver tecnologia de custo-benefício aceitável para ajuste/remoção do seu excesso. A ingestão diária de água com fluoreto em concentração > 0,9 mg/L representa risco à dentição em menores de oito anos de idade e os consumidores deveriam ser expressamente informados desse risco. Considerando a expansão do programa nacional de fluoretação da água para regiões de clima tipicamente tropical, deve-se revisar a Portaria 635/75, relacionada ao fluoreto adicionado às águas de abastecimento público.

  17. Aspectos qualitativos da água do Rio Poty na região de Teresina, PI

    Directory of Open Access Journals (Sweden)

    Lisânea Mycheline Oliveira Damasceno

    Full Text Available Resumo O presente trabalho objetivou monitorar e avaliar a qualidade da água do Rio Poty, em Teresina (PI para fins agrícolas em períodos extremos de temperatura e de precipitação. Coletaram-se amostras de água no rio em 10 pontos georreferenciados, da curva do conjunto residencial São Paulo (zona sudeste até sua foz no Rio Parnaíba (zona norte, em um percurso aproximado de 23,7 km, de junho a dezembro de 2004. Determinou-se as variáveis físico-químicas: CEa, RAS e pH, além das concentrações de Cl-, HCO3-, CO3 2-, Na+, Ca2+ e Mg2+. Os maiores valores da CEa (0,26 dS m-1 e da RAS (1,90 mmolc L-10,5 foram registradas no mês de outubro, devido ao baixo índice pluviométrico, período seco e eventual poluição antrópica. Pelo Teste de Kruskal-Wallis, constataram-se oscilações nas concentrações de HCO3- (1,68 a 1,91 mmolc L-1 ao longo dos pontos amostrais. O carbonato de sódio residual (CSR indicou que a água é apropriada para fins agrícolas (< 1,25 mmolcL-1.

  18. Sediment pollution in margins of the Lake Guaíba, Southern Brazil.

    Science.gov (United States)

    de Andrade, Leonardo Capeleto; Tiecher, Tales; de Oliveira, Jessica Souza; Andreazza, Robson; Inda, Alberto Vasconcellos; de Oliveira Camargo, Flávio Anastácio

    2017-12-02

    Sediments are formed by deposition of organic and inorganic particles on depth of water bodies, being an important role in aquatic ecosystems, including destination and potential source of essential nutrients and heavy metals, which may be toxic for living organisms. The Lake Guaíba supplies water for approximately two million people and it is located in the metropolitan region of Porto Alegre, Rio Grande do Sul State, Brazil. Thus, the aim of this study was to evaluate the sediment pollution in the margins of Lake Guaíba in the vicinity of Porto Alegre city. Surface sediment was sampled in 12 sites to assess the concentration of several elements (C, N, P, Fe, Al, Ca, Mg, Na, K, Mn, Ba, Zn, V, Pb, Cu, Cr, Ni, Cd, Mo, and Se) and the mineralogical composition. Sediment in margins of Lake Guaíba presented predominantly (> 95%) sandy fraction in all samples, but with significant differences between evaluated sites. Sediments in the margins of Lake Guaíba showed indications of punctual water pollution with Pb, Cu, Cr, Ni, TOC, TKN, and P, mainly derived from urban streams that flow into the lake. In order to solve these environmental liabilities, public actions should not focus only on Guaíba, but also in the streams that flow into the lake.

  19. The N-Myc down regulated Gene1 (NDRG1) Is a Rab4a effector involved in vesicular recycling of E-cadherin.

    Science.gov (United States)

    Kachhap, Sushant K; Faith, Dennis; Qian, David Z; Shabbeer, Shabana; Galloway, Nathan L; Pili, Roberto; Denmeade, Samuel R; DeMarzo, Angelo M; Carducci, Michael A

    2007-09-05

    Cell to cell adhesion is mediated by adhesion molecules present on the cell surface. Downregulation of molecules that form the adhesion complex is a characteristic of metastatic cancer cells. Downregulation of the N-myc down regulated gene1 (NDRG1) increases prostate and breast metastasis. The exact function of NDRG1 is not known. Here by using live cell confocal microscopy and in vitro reconstitution, we report that NDRG1 is involved in recycling the adhesion molecule E-cadherin thereby stabilizing it. Evidence is provided that NDRG1 recruits on recycling endosomes in the Trans Golgi network by binding to phosphotidylinositol 4-phosphate and interacts with membrane bound Rab4aGTPase. NDRG1 specifically interacts with constitutively active Rab4aQ67L mutant protein and not with GDP-bound Rab4aS22N mutant proving NDRG1 as a novel Rab4a effector. Transferrin recycling experiments reveals NDRG1 colocalizes with transferrin during the recycling phase. NDRG1 alters the kinetics of transferrin recycling in cells. NDRG1 knockdown cells show a delay in recycling transferrin, conversely NDRG1 overexpressing cells reveal an increase in rate of transferrin recycling. This novel finding of NDRG1 as a recycling protein involved with recycling of E-cadherin will aid in understanding NDRG1 role as a metastasis suppressor protein.

  20. The N-Myc down regulated Gene1 (NDRG1 Is a Rab4a effector involved in vesicular recycling of E-cadherin.

    Directory of Open Access Journals (Sweden)

    Sushant K Kachhap

    2007-09-01

    Full Text Available Cell to cell adhesion is mediated by adhesion molecules present on the cell surface. Downregulation of molecules that form the adhesion complex is a characteristic of metastatic cancer cells. Downregulation of the N-myc down regulated gene1 (NDRG1 increases prostate and breast metastasis. The exact function of NDRG1 is not known. Here by using live cell confocal microscopy and in vitro reconstitution, we report that NDRG1 is involved in recycling the adhesion molecule E-cadherin thereby stabilizing it. Evidence is provided that NDRG1 recruits on recycling endosomes in the Trans Golgi network by binding to phosphotidylinositol 4-phosphate and interacts with membrane bound Rab4aGTPase. NDRG1 specifically interacts with constitutively active Rab4aQ67L mutant protein and not with GDP-bound Rab4aS22N mutant proving NDRG1 as a novel Rab4a effector. Transferrin recycling experiments reveals NDRG1 colocalizes with transferrin during the recycling phase. NDRG1 alters the kinetics of transferrin recycling in cells. NDRG1 knockdown cells show a delay in recycling transferrin, conversely NDRG1 overexpressing cells reveal an increase in rate of transferrin recycling. This novel finding of NDRG1 as a recycling protein involved with recycling of E-cadherin will aid in understanding NDRG1 role as a metastasis suppressor protein.

  1. Modelação da dinâmica da água e dos sais num Aluviossolo regado com águas de diferente qualidade: ensaio de validação do modelo HYDRUS-1D com observações em monólitos Water and solute transport simulation in a Fluvisol irrigated with waters of different quality: testing the HYDRUS-1D model with observations in soil monoliths

    Directory of Open Access Journals (Sweden)

    M. C. Gonçalves

    2007-07-01

    Full Text Available Entre 2001 e 2004 procedeu-se à monitorização dos teores de água e dos catiões solúveis Na+, Ca2+ e Mg2+, às profundidades de 10, 30, 50 e 70 cm, em três monólitos de solo (1.2 m² x 1.0 m de profundidade, construídos num Aluviossolo de textura mediana, situado em Alvalade de Sado, visando o estudo da evolução da salinização e da sodicização do solo em função de diferentes qualidades de água de rega aplicadas. Os monólitos, cobertos com vegetação natural, foram regados manualmente (10 mm por aplicação, durante o ciclo de rega da região (500 mm e sujeito à lava-gem pela chuva (entre 388 e 570 mm/ano durante o período remanescente do ano. As águas de rega inicialmente aplicadas apresentavam uma CE de 0.4, 0.8 e 1.6 dS m-1 e um valor de SAR de 1, 3 e 6 (meq L-10,5, designadas de A, B e C, respectivamente, mantendo sempre uma relação Ca:Mg = 1:2. No 3º e 4º anos optou-se por aumentar a CE das águas de rega para 0.8, 1,6 e 3.2 dS m-1, mantendo o respectivo equilíbrio entre os catiões. O teor de água no solo foi monitorizado às 4 profundidades por TDR e a solução de solo foi amostrada recorrendo a cápsulas porosas instaladas às mesmas profundidades. Foi monitorizada a evolução dos teores de água e de Na+, Ca2+ e Mg2+ solúveis, da condutividade eléctrica (CE, da razão de adsorção de sódio (SAR e da percentagem de sódio de troca (ESP nos três monólitos, às quatro profundidades e ao longo dos 4 anos de ensaio. A modelação dos processos envolvidos foi efectuada recorrendo ao modelo HYDRUS-1D, o qual se mostrou adequado à simulação dos resultados experimentais. A análise entre os valores medidos e simulados foi feita com recurso à análise de regressão simples, forçada à origem. As equações obtidas apresentaram coeficientes de regressão próximos de 1 e coeficientes de determinação elevados, variando entre 0.985 para o teor de água e 0.797 para o cálcio solúvel. Pode-se considerar que

  2. Temporal expression of genes involved in the biosynthesis of ...

    African Journals Online (AJOL)

    Gibberellins (GAs) are a large family of endogenous plant growth regulators. Bioactive GAs influence nearly all processes during plant growth and development. In the present study, we cloned and identified 10 unique genes that are potentially involved in the biosynthesis of GAs, including one BpGGDP gene, two BpCPS ...

  3. Modelagem da intrusão da água de um rio com diferentes concentrações de sais

    Directory of Open Access Journals (Sweden)

    Gabriel C. Marino

    2015-01-01

    Full Text Available Para considerações de intrusão de água em meio subterrâneo a capilaridade, também entendida como força de sucção, é uma grandeza física que influencia a dinâmica do processo. Neste trabalho é apresentada uma investigação do comportamento do escoamento subterrâneo e as interações entre água doce e água salinizada na área de descarga de um rio. Objetivou-se realizar uma modelagem preditiva para identificar e representar o processo de intrusão das águas de um rio meio ao lençol freático comparando diferentes concentrações de sais nas águas do rio e águas subterrâneas. Como modelo conceitual foi definido um rio em região de descarga. A modelagem foi realizada no software SUTRA sendo o método de solução por elementos finitos. Observou-se a formação de uma camada de água doce sobre o lençol freático salinizado quando considerada intrusão de água doce. Para as considerações com intrusão de água salinizada foi identificada a formação de fingers. Os resultados do modelo possibilitaram identificar que a intrusão de água doce no lençol freático salinizado é dificultada pela presença de sais. A intrusão é mais difícil quanto maior a concentração de sais nas águas subterrâneas.

  4. Genes involved in translation of Mycoplasma hyopneumoniae and Mycoplasma synoviae

    Directory of Open Access Journals (Sweden)

    Mônica de Oliveira Santos

    2007-01-01

    Full Text Available This is a report on the analysis of genes involved in translation of the complete genomes of Mycoplasma hyopneumoniae strain J and 7448 and Mycoplasma synoviae. In both genomes 31 ORFs encoding large ribosomal subunit proteins and 19 ORFs encoding small ribosomal subunit proteins were found. Ten ribosomal protein gene clusters encoding 42 ribosomal proteins were found in M. synoviae, while 8 clusters encoding 39 ribosomal proteins were found in both M. hyopneumoniae strains. The L33 gene of the M. hyopneumoniae strain 7448 presented two copies in different locations. The genes encoding initiation factors (IF-1, IF-2 and IF-3, elongation factors (EF-G, EF-Tu, EF-Ts and EF-P, and the genes encoding the ribosome recycling factor (frr and one polypeptide release factor (prfA were present in the genomes of M. hyopneumoniae and M. synoviae. Nineteen aminoacyl-tRNA synthases had been previously identified in both mycoplasmas. In the two strains of M. hyopneumoniae, J and 7448, only one set of 5S, 16S and 23S rRNAs had been identified. Two sets of 16S and 23S rRNA genes and three sets of 5S rRNA genes had been identified in the M. synoviae genome.

  5. Serviços logísticos como diferencial competitivo: o caso da água mineral

    OpenAIRE

    Vontobel, Otto

    2007-01-01

    Esta dissertação apresenta um estudo sobre o mercado de consumo residencial de água mineral, em embalagem retornável de 20 litros, na cidade de Porto Alegre, tendo como foco o setor de distribuição de água e os serviços logísticos associados. Este tipo de embalagem tem aumentado sua participação, que já representa mais de 50% no crescente volume de água mineral comercializado no Brasil. O crescimento da população em áreas metropolitanas dificulta o tratamento da água pública, e em busca de ág...

  6. Discovery of a novel gene involved in autolysis of Clostridium cells.

    Science.gov (United States)

    Yang, Liejian; Bao, Guanhui; Zhu, Yan; Dong, Hongjun; Zhang, Yanping; Li, Yin

    2013-06-01

    Cell autolysis plays important physiological roles in the life cycle of clostridial cells. Understanding the genetic basis of the autolysis phenomenon of pathogenic Clostridium or solvent producing Clostridium cells might provide new insights into this important species. Genes that might be involved in autolysis of Clostridium acetobutylicum, a model clostridial species, were investigated in this study. Twelve putative autolysin genes were predicted in C. acetobutylicum DSM 1731 genome through bioinformatics analysis. Of these 12 genes, gene SMB_G3117 was selected for testing the in tracellular autolysin activity, growth profile, viable cell numbers, and cellular morphology. We found that overexpression of SMB_G3117 gene led to earlier ceased growth, significantly increased number of dead cells, and clear electrolucent cavities, while disruption of SMB_G3117 gene exhibited remarkably reduced intracellular autolysin activity. These results indicate that SMB_G3117 is a novel gene involved in cellular autolysis of C. acetobutylicum.

  7. Qualidade de águas residuárias e salobra utilizadas no cultivo hidropônico de três cultivares de alface crespa

    Directory of Open Access Journals (Sweden)

    José Emídio de Albuquerque Júnior

    2016-04-01

    Full Text Available O reúso de efluentes tratados não é uma prática nova, no entanto, há um interesse crescente em relação à necessidade de sua reutilização. Objetivou-se avaliar a caraterização das águas residuárias domestica e do reator UASB e água salobra de poço para o cultivo hidropônico de alface crespa. O experimento foi conduzido em ambiente protegido com sistema hidropônico. Delineamento experimental foi em blocos casualizados com esquema fatorial 7 x 3, três repetições sendo, 7 soluções hidropônicase três cultivares de alface. A parcela experimental, S1=solução de Furlani; S2=água residuária domestica; S3=água residuária domestica otimizada; S4=água de poço; S5=água de poço otimizada; S6=água residuária (UASB e S7=água residuária (UASB otimizada, subparcela três cultivares de alface. O pH das águas residuárias foram de 7,7 e 7,4 (Poço; 7,4 e 8,0 (Esgoto bruto e, 7,2 e 8,2 (Extrabes. A (CE foram 0,957 e 1,002 (Poço; 2,133 e 2,368 (Esgoto bruto e (Extrabes 2,502 e 2,4 09 dS m-1. O uso de soluções minerais nutritivas utilizando água residuária é viável quando utilizado em sistemas hidropônicos, sendo indicado para principalmente para região do semiárido brasileiro e quando se faz uso de água de irrigação de qualidade inferior na produção de hortaliças.Quality of wastewater and brackish water used in the hydroponic cultivation of three varieties of curly lettuce Abstract: The reuse of treated wastewater is not a new practice, however, there is a growing interest in the need for re-use. The objective was to evaluate the Characterisation of domestic wastewater and UASB and brackish well for hydroponic lettuce. The experiment was conducted in a protected environment with hydroponically. Experimental design was a randomized block with factorial 7 x 3, three replications and 7 hydroponic solutions and three lettuce cultivars. The experimental portion, S1 = Furlani solution; S2 = domestic wastewater; S3

  8. Dessalinização da água do mar

    OpenAIRE

    Diogo, Maria Alice Antunes Pinto

    2013-01-01

    Através deste relatório de estágio, pretende-se apresentar uma alternativa para a escassez de água potável, um problema com que a humanidade atualmente se debate. A dessalinização da água do mar apresenta-se como uma das soluções possíveis para a humanidade vencer mais esta crise que já se pronuncia. Para melhor compreensão do tema e esclarecimento dos fatos, foi elaborada uma pesquisa bibliográfica, em livros, artigos, teses, internet, entre outros. Este trabalho propõe-se fornecer inform...

  9. Avaliação da nanofiltração e da osmose inversa na remoção de carbofurano em águas de abastecimento

    Directory of Open Access Journals (Sweden)

    Marcelo Zawadzki Bueno

    Full Text Available RESUMO: A complexidade físico-química de micropoluentes, como os defensivos agrícolas, exige o uso de tecnologias avançadas de tratamento de água para abastecimento. O objetivo deste estudo foi avaliar a remoção de carbofurano em nível de bancada (batelada utilizando membranas comerciais de nanofiltração (NF90 - DowFilmtec (r e osmose inversa (HR - Koch Membrane Systems(r . Nos experimentos foi avaliada a influência da qualidade da matriz de alimentação (água ultrapura, bruta e pré-tratada contendo carbofurano (50 µgL-1, e da pressão de operação (8 e 15 bar para a NF90 e 15 e 30 bar para a HR. Para a NF90, com a matriz de água ultrapura, as eficiências de remoção foram inferiores (89,8%, porém o fluxo permeado foi superior (132,1 Lm-2h-1 àquelas obtidas com a matriz de água bruta (98,4% - 94,7 Lm-2h-1 e água pré-tratada (95,2% - 95,3 Lm-2h-1 para a pressão de 15 bar. Para a membrana HR, remoções relativamente superiores foram observadas para a pressão de 30 bar para as três matrizes, com a água bruta apresentando os melhores resultados (99,7% - 49,7 Lm-2h-1. Concluiu-se, portanto, que o pré-tratamento da água bruta não acarretou diferença significativa na remoção do carbofurano e não influenciou no fluxo de permeado de acordo com a metodologia adotada (baixo tempo de separação em batelada com recirculação.

  10. Qualidade da água da Lagoa do Josino, em Formiga (MG

    Directory of Open Access Journals (Sweden)

    Joaquim Cordeiro Neto

    2017-07-01

    Full Text Available A água é o recurso natural mais importante da Terra, sendo indispensável para a vida, assim, sua qualidade deve ser preservada. A partir disso, este trabalho tem por objetivo analisar a qualidade da água da Lagoa do Josino, localizada no município de Formiga (MG, a partir de análises físicas, químicas e microbiológicas. Para tal, foram coletadas amostras de água em diversos pontos da Lagoa do Josino, que apresenta diferentes formas de uso e ocupação em suas imediações. Foram analisados os seguintes parâmetros, entre os meses de agosto e outubro de 2015, pH, condutividade elétrica, temperatura, oxigênio dissolvido, turbidez, nitrato, nitrito, amônia, nitrogênio total, potássio, fósforo, ferroII, ferroIII, demanda bioquímica de oxigênio (DBO, sólidos totais, sólidos dissolvidos totais, sólidos sedimentáveis, coliformes termotolerantes e totais, em cinco repetições. Os resultados obtidos para DBO, fósforo total, coliformes termotolerantes e totais não estão de acordo com o preconizado pela Resolução 357/2005 do CONAMA para Corpos Hídricos Classe 1 e pela Resolução 274/2000, do mesmo conselho.

  11. CCDB: a curated database of genes involved in cervix cancer.

    Science.gov (United States)

    Agarwal, Subhash M; Raghav, Dhwani; Singh, Harinder; Raghava, G P S

    2011-01-01

    The Cervical Cancer gene DataBase (CCDB, http://crdd.osdd.net/raghava/ccdb) is a manually curated catalog of experimentally validated genes that are thought, or are known to be involved in the different stages of cervical carcinogenesis. In spite of the large women population that is presently affected from this malignancy still at present, no database exists that catalogs information on genes associated with cervical cancer. Therefore, we have compiled 537 genes in CCDB that are linked with cervical cancer causation processes such as methylation, gene amplification, mutation, polymorphism and change in expression level, as evident from published literature. Each record contains details related to gene like architecture (exon-intron structure), location, function, sequences (mRNA/CDS/protein), ontology, interacting partners, homology to other eukaryotic genomes, structure and links to other public databases, thus augmenting CCDB with external data. Also, manually curated literature references have been provided to support the inclusion of the gene in the database and establish its association with cervix cancer. In addition, CCDB provides information on microRNA altered in cervical cancer as well as search facility for querying, several browse options and an online tool for sequence similarity search, thereby providing researchers with easy access to the latest information on genes involved in cervix cancer.

  12. Análise dos fatores que influenciam o consumo de água mineral

    OpenAIRE

    Pitaluga, Christiane Marques

    2006-01-01

    A água mineral foi o produto que mais apresentou crescimento em consumo entre as famílias brasileiras nos últimos trinta anos, ultrapassando 0,320 litros per capita/ano em 1974/1975 e alcançando a marca de 18,541 litros em 2002/2003. Dentre os produtos de maior consumo alimentar, somente o leite fica à frente da água mineral. No ambito mundial, o consumo de água envasada continuará crescendo nos próximos anos, passando de um volume de 155 bilhões de litros em 2003 para 206 bilhões em 2008, se...

  13. Glosa especulação bastarda ao redor da água

    Directory of Open Access Journals (Sweden)

    Leopoldo Waizbort

    2000-11-01

    Full Text Available Debruço-me sobre um artigo anteriormente publicado em Tempo social, de autoria de José Carlos Bruni, na tentativa de "compreender a água enquanto água" e as implicações de tal empresa.I dwell upon an article previously published in Tempo Social, by José Carlos Bruni, in the hope of "understanding water as water" and the implications of such an edeavour.

  14. Qualidade da água no consórcio de peixes com arroz irrigado

    Directory of Open Access Journals (Sweden)

    Golombieski Jaqueline Ineu

    2005-01-01

    Full Text Available O objetivo deste trabalho foi avaliar a qualidade da água utilizada no consórcio de peixes com arroz irrigado. O experimento foi conduzido em área de várzea, com sistema pré-germinado de cultivo de arroz associado ao cultivo de carpas e jundiá, onde semanalmente foram monitorados os níveis de oxigênio dissolvido, temperatura, pH, dureza, alcalinidade total, amônia, nitrito e transparência da água. As características físico-químicas da água (temperatura, pH, dureza, alcalinidade,amônia e nitrito não afetam o crescimento e o desenvolvimento dos peixes no consórcio com arroz irrigado para as espécies cultivadas (60% de carpa húngara, 20% de carpa capim, 10% de jundiá, 5% de carpa prateada e 5% de carpa cabeça grande. Embora as densidades de peixes utilizadas e as épocas de colocação dos alevinos afetem certos parâmetros de qualidade da água (oxigênio dissolvido e transparência da água em alguns períodos, em geral eles se mantêm em níveis adequados de acordo com os padrões para as espécies.

  15. O reúso da água e sua possibilidade na região metropolitana de São Paulo

    OpenAIRE

    Pedro Caetano Sanches Mancuso

    1992-01-01

    Inicialmente este trabalho conceitua reuso de água, apresentando os critérios e padrões de qualidade de água, para suas várias modalidades. Em seguida estuda a oferta e a demanda de água na Região Metropolitana de São Paulo, bem como apresenta uma retrospectiva das principais crises no abastecimento da região, em termos de quantidade e qualidade de água. Finalmente, depois de resumir os principais casos de reuso de água em outros países e em São Paulo, o trabalho sugere uma estratégia para o ...

  16. Avaliação da qualidade da água proveniente de estações de tratamento de água salobra na zona rural do município de Mossoró, RN

    OpenAIRE

    Cosme, Christiano Rebouças

    2011-01-01

    No Brasil, principalmente na região semiárida, um grande desafio é promover o abastecimento de água às famílias residentes nas áreas rurais. As águas subterrâneas são apontadas como uma alternativa viável para garantir o acesso das comunidades rurais do nordeste à água, a partir de investimentos públicos na perfuração de poços tubulares. Entretanto, essas fontes hídricas apresentam na maioria dos casos restrições de uso para dessedentamento humano, por apresentarem problemas de...

  17. Aproveitamento de Água de Chuva em unidades Educacionais do Rio Grande do Norte, Brasil

    Directory of Open Access Journals (Sweden)

    Xaila Sant Anna Amaral

    2017-12-01

    Full Text Available Com este estudo objetivou-se avaliar os sistemas de aproveitamento de água de chuva em unidades educacionais do Rio Grande do Norte. Inicialmente, foi realizado um levantamento de informações sobre os sistemas de aproveitamento de água de chuva dos campi do IFRN. Em seguida, os campi foram visitados para validação das informações colhidas e verificação de outros elementos pertinentes (áreas da superfície de captação, tipos e volumes de reservatórios, estado físico das instalações, condições operacionais e destino da água armazenada. Posteriormente, 4 unidades educacionais foram selecionadas e monitoradas mensalmente entre março e setembro de 2014 a fim de caracterizar a água de chuva armazenada, físico-química e microbiologicamente. Os resultados revelam que a água armazenada apresentou qualidade compatível para irrigação de áreas verdes, apesar de descuidos operacionais e sanitários. Conclui-se que sistemas de captação e armazenamento podem compatibilizar o uso racional, eficiente e sanitário da água de chuva no Semiárido brasileiro.

  18. Estimativa da capacidade de campo pela curva de retenção e pela densidade de fluxo da água

    Directory of Open Access Journals (Sweden)

    Alexsandro dos Santos Brito

    2011-12-01

    Full Text Available A capacidade de campo é um parâmetro de inegável relevância para o manejo adequado da irrigação. A partir da determinação confiável do conteúdo de água no solo na capacidade de campo, pode-se otimizar a produtividade das culturas agrícolas, maximizando a eficiência do uso da água pelas plantas e evitando a contaminação do lençol freático por lixiviação de fertilizantes e agroquímicos. Nesse sentido, o trabalho teve como objetivo avaliar a capacidade de campo (a pelo cálculo da densidade de fluxo da água durante o processo de redistribuição no experimento clássico de determinação desse parâmetro e (b a partir de uma dada tensão da água na curva de retenção, em um Latossolo Vermelho-Amarelo textura média, localizado em área experimental da Escola Superior de Agricultura Luiz de Queiroz, município de Piracicaba, Estado de São Paulo (Brasil. Para isso, instalaram-se no solo, até a profundidade de 1,9 m, 60 tubos de acesso a uma sonda de nêutrons, numa rede de malha quadrada de 5 m (6 x 10 pontos amostrais, e, adjacente a cada tubo, dois tensiômetros, um a 0,75 m e outro a 0,85 m de profundidade. Nos 60 pontos (locais amostrais, desenvolveu-se um experimento similar ao experimento clássico de determinação da capacidade de campo, no qual o solo foi devidamente submetido a uma lâmina de infiltração visando à saturação do seu perfil; sua superfície, coberta com lona plástica para evitar fluxo de água através dela; e a redistribuição da água, monitorada até a profundidade de 0,8 m durante 20 dias. Observou-se que o conteúdo de água correspondente a uma densidade de fluxo de 1,0 mm dia-1 é a melhor estimativa da capacidade de campo para esse solo, uma vez que as densidades de fluxo de 0,1 e 0,01 mm dia-1, também recomendadas para estimar a capacidade de campo, são muito baixas, a ponto de não terem sido alcançadas neste estudo. Quanto aos resultados obtidos pelo método baseado na curva de

  19. Gene expression profile analysis of Ligon lintless-1 (Li1) mutant reveals important genes and pathways in cotton leaf and fiber development.

    Science.gov (United States)

    Ding, Mingquan; Jiang, Yurong; Cao, Yuefen; Lin, Lifeng; He, Shae; Zhou, Wei; Rong, Junkang

    2014-02-10

    Ligon lintless-1 (Li1) is a monogenic dominant mutant of Gossypium hirsutum (upland cotton) with a phenotype of impaired vegetative growth and short lint fibers. Despite years of research involving genetic mapping and gene expression profile analysis of Li1 mutant ovule tissues, the gene remains uncloned and the underlying pathway of cotton fiber elongation is still unclear. In this study, we report the whole genome-level deep-sequencing analysis of leaf tissues of the Li1 mutant. Differentially expressed genes in leaf tissues of mutant versus wild-type (WT) plants are identified, and the underlying pathways and potential genes that control leaf and fiber development are inferred. The results show that transcription factors AS2, YABBY5, and KANDI-like are significantly differentially expressed in mutant tissues compared with WT ones. Interestingly, several fiber development-related genes are found in the downregulated gene list of the mutant leaf transcriptome. These genes include heat shock protein family, cytoskeleton arrangement, cell wall synthesis, energy, H2O2 metabolism-related genes, and WRKY transcription factors. This finding suggests that the genes are involved in leaf morphology determination and fiber elongation. The expression data are also compared with the previously published microarray data of Li1 ovule tissues. Comparative analysis of the ovule transcriptomes of Li1 and WT reveals that a number of pathways important for fiber elongation are enriched in the downregulated gene list at different fiber development stages (0, 6, 9, 12, 15, 18dpa). Differentially expressed genes identified in both leaf and fiber samples are aligned with cotton whole genome sequences and combined with the genetic fine mapping results to identify a list of candidate genes for Li1. Copyright © 2013 Elsevier B.V. All rights reserved.

  20. Absorção de água pelas plantas: água disponível versus extraível e a produtividade das culturas Water absorption by plants: available versus extractable soil water and crop production

    Directory of Open Access Journals (Sweden)

    Reimar Carlesso

    1995-01-01

    Full Text Available Um aspecto importante, no entendimento da relação entre a absorção de água e a produção das culturas, é como ocorre o balanço no suprimento de água do solo para satisfazer a demanda evaporativa da atmosfera. O suprimento de água é determinado pela habilidade da cultura em utilizar a água armazenada no solo e, a demanda, por outro lado, é determinada pela combinação dos fatores meteorológicos interagindo com o "dossel" vegetativo da cultura. Apesar da abundante literatura disponível, diferentes critérios tem sido utilizados na determinação do momento de ocorrência e da duração de um déficit hídrico. Muita ênfase tem sido dada para a quantificação do consumo de água pelas plantas nos diferentes subperíodos de desenvolvimento e reduzida importância tem sido conferida a quantificação da quantidade de água disponível (extraível e seus efeitos no crescimento e desenvolvimento das plantas. Na discussão sobre a extração de água pelas plantas deve-se considerar, primeiramente, o problema da precisão na definição dos limites de disponibilidade de água. Esta publicação apresenta uma análise crítica das limitações normalmente encontradas na utilização dos limites de disponibilidade de água. A relação entre a água disponível e a água extraível do solo é analisada a partir de resultados sobre a taxa de transpiração e a tração de água extraível, a profundidade efetiva de extração do sistema radicular e, a extração de água por unidade de profundidade.An important issue to understanding the effect of water shortage on crop yield is how crops balance the supply of water from the soil to match the demand for water from the atmosphere. Supply is determined by the crop ability to utilise the stored soil water and, demand, on the other hand, is determined by a combination of metereological factors interacting with the crop canopy. Different criteria have been used to determine the timing and

  1. Alu Elements as Novel Regulators of Gene Expression in Type 1 Diabetes Susceptibility Genes?

    Science.gov (United States)

    Kaur, Simranjeet; Pociot, Flemming

    2015-07-13

    Despite numerous studies implicating Alu repeat elements in various diseases, there is sparse information available with respect to the potential functional and biological roles of the repeat elements in Type 1 diabetes (T1D). Therefore, we performed a genome-wide sequence analysis of T1D candidate genes to identify embedded Alu elements within these genes. We observed significant enrichment of Alu elements within the T1D genes (p-value genes harboring Alus revealed significant enrichment for immune-mediated processes (p-value genes harboring inverted Alus (IRAlus) within their 3' untranslated regions (UTRs) that are known to regulate the expression of host mRNAs by generating double stranded RNA duplexes. Our in silico analysis predicted the formation of duplex structures by IRAlus within the 3'UTRs of T1D genes. We propose that IRAlus might be involved in regulating the expression levels of the host T1D genes.

  2. Análise econômica de um sistema de aproveitamento de água de chuva em unidade hospitalar

    OpenAIRE

    Fonseca, Márcio de Castro

    2016-01-01

    O uso de tecnologias de aproveitamento de água de chuva vem se consolidando cada vez mais como fonte alternativa de suprimento de água em diversos tipos de usos. A vantagem desta tecnologia se baseia na acessibilidade da água durante a estação seca, com a possibilidade de substituição da água proveniente do abastecimento público. O objetivo deste estudo consistiu na análise financeira de dois projetos piloto de aproveitamento de água de chuva para o abastecimento de lavanderia em unidade hosp...

  3. Método computacional para correção da alcalinidade de águas para fertirrigação

    Directory of Open Access Journals (Sweden)

    F. B. Egreja Filho

    1999-06-01

    Full Text Available A alcalinidade excessiva das águas destinadas à fertirrigação pode criar uma série de inconvenientes, que vão desde o entupimento dos emissores, pela precipitação de carbonatos, até a redução da disponibilidade dos micronutrientes para as culturas. Com o propósito de auxiliar a tomada de decisão quanto ao manejo da alcalinidade dessas águas, desenvolveu-se o ALKA 1.0, um software em CLIPPER 5.2, que pode ser rodado em microcomputadores IBM-PC/XT/AT/PENTIUM ou outros 100% compatíveis. A partir do fornecimento do pH original da água de irrigação, da sua alcalinidade total ou subdividida em CO3(2- e HCO3- (em mmol L-1, e da lâmina de irrigação utilizada durante o ciclo da cultura, o ALKA 1.0 fornece as quantidades de HCl, HNO3, H2SO4 e H3PO4 que devem ser adicionadas à água de irrigação para que o seu pH atinja o valor estipulado pelo usuário. Além do volume de cada ácido a ser adicionado, o programa fornece a quantidade de nutrientes introduzidas pelos ácidos, na dosagem recomendada, bem como o custo total de cada uma das possíveis escolhas. Para tal, cadastram-se, inicialmente, a densidade e a pureza (%p/p de cada ácido, bem como seu custo por litro. O ALKA 1.0 oferece, ainda, a opção de adições mistas dos diversos ácidos, visando não só à remoção da alcalinidade excessiva da água, mas também a um balanço adequado à cultura em questão dos nutrientes (N, P, S adicionados via ácido. Por fim, o ALKA 1.0 oferece a possibilidade de trabalhar-se com bancos de dados, pré-cadastrados, que contêm informações sobre as origens (localidades e fontes (rios, poços, lagos, açudes e outros de diversas águas, além do seu pH e alcalinidade total. Desta forma, podem-se conhecer as características de manejo da alcalinidade dessas águas em função de características definidas pelo usuário, tais como um município em particular, determinada fonte, pH, e outros. O ALKA 1.0 foi validado com uma mistura sint

  4. QUALIDADE BACTERIOLÓGICA DE ÁGUA MINERAL COMERCIALIZADA EM TANGARÁ DA SERRA-MT

    OpenAIRE

    Bruno Wagner ZAGO; Ilio Fealho de CARVALHO; Maurecilne Lemes da Silva CARVALHO

    2013-01-01

    Diante da constante preocupação com a qualidade de água distribuída para a população, a água mineral se tornou o alvo de pessoas que procuram um estilo de vida saudável, tornando-se então um interessante objeto de pesquisa. Nestas condições, para uma verificação da qualidade da água mineral comercializada na cidade de Tangará da Serra - MT, foi realizada a quantificação de coliformes totais, termotolerantes e bactérias aeróbias mesófilas, além da medição do pH e temper...

  5. Consumos de água em edifícios universitários: caso do DECivil da UA

    OpenAIRE

    Gonçalves, Pedro Jorge Pereira Amaral

    2014-01-01

    Segundo o Plano Nacional para o Uso Eficiente da Água em Portugal (PNUEA, 2012), existe a possibilidade de ocorrerem situações de stress hídrico se não se melhorar o uso da água no sentido de uma conservação dos recursos hídricos. Assim, é necessário estudar os usos de água para se saber onde intervir por forma a preservar os recursos hídricos. Parte da água é consumida em meio urbano pelo que, para aumentar a eficiência hídrica das cidades, é necessário conhecer primeiro as característica...

  6. Population genetics and comparative genetics of CLDN1, a gene involved in hepatitis C virus entry

    NARCIS (Netherlands)

    Bekker, Vincent; O'Brien, Thomas R.; Chanock, Stephen

    2009-01-01

    The claudin-1 gene (CLDN1) is a member of a family of genes that encodes proteins found in tight junctions and it has recently been implicated as one of several receptors for late stage binding of hepatitis C virus (HCV). Exploration of the population genetics of this gene could be informative,

  7. Naringenin Regulates Expression of Genes Involved in Cell Wall Synthesis in Herbaspirillum seropedicae▿

    Science.gov (United States)

    Tadra-Sfeir, M. Z.; Souza, E. M.; Faoro, H.; Müller-Santos, M.; Baura, V. A.; Tuleski, T. R.; Rigo, L. U.; Yates, M. G.; Wassem, R.; Pedrosa, F. O.; Monteiro, R. A.

    2011-01-01

    Five thousand mutants of Herbaspirillum seropedicae SmR1 carrying random insertions of transposon pTnMod-OGmKmlacZ were screened for differential expression of LacZ in the presence of naringenin. Among the 16 mutants whose expression was regulated by naringenin were genes predicted to be involved in the synthesis of exopolysaccharides, lipopolysaccharides, and auxin. These loci are probably involved in establishing interactions with host plants. PMID:21257805

  8. Naringenin regulates expression of genes involved in cell wall synthesis in Herbaspirillum seropedicae.

    Science.gov (United States)

    Tadra-Sfeir, M Z; Souza, E M; Faoro, H; Müller-Santos, M; Baura, V A; Tuleski, T R; Rigo, L U; Yates, M G; Wassem, R; Pedrosa, F O; Monteiro, R A

    2011-03-01

    Five thousand mutants of Herbaspirillum seropedicae SmR1 carrying random insertions of transposon pTnMod-OGmKmlacZ were screened for differential expression of LacZ in the presence of naringenin. Among the 16 mutants whose expression was regulated by naringenin were genes predicted to be involved in the synthesis of exopolysaccharides, lipopolysaccharides, and auxin. These loci are probably involved in establishing interactions with host plants.

  9. Efeito da fotossíntese na alcalinidade da água de cultivo da tilápia do Nilo

    Directory of Open Access Journals (Sweden)

    Davi de Holanda Cavalcante

    Full Text Available Resumo As características físicas e químicas da água de cultivo afetam fortemente o crescimento, a reprodução, a saúde e a sobrevivência dos peixes. Por isso, delineou-se o presente trabalho com o objetivo de avaliar os efeitos da atividade fotossintética na alcalinidade total da água de cultivo de alevinos de tilápia do Nilo Oreochromis niloticus mantidos em laboratório. Havia 18 aquários de polietileno com capacidade total para 25 L e volume útil de 20 L, distribuídos igualmente em dois sistemas de cultivo (interno e externo. No início do experimento, quinze alevinos de 0,52 ± 0,06 g foram estocados em cada aquário de cultivo, tanto no sistema interno como externo. O sistema de cultivo externo (águas verdes foi considerado o tratamento experimental do trabalho, tendo o sistema de cultivo interno (águas claras como controle experimental. O experimento teve a duração de 6 semanas. Semanalmente, coletaram-se amostras de água de todas as unidades experimentais para determinação da alcalinidade total e leitura do pH. Os resultados foram submetidos à análise de variância (ANOVA para experimentos inteiramente casualizados, adotando-se 5% como nível de significância. Em média, o pH da água nos aquários sem e com fitoplâncton foi de 7,32 ± 0,39 e 8,45 ± 0,40, respectivamente, havendo diferença significativa entre estes resultados (P < 0,001. A alcalinidade total média no sistema de aquários com águas claras e verdes foi de 50,49 ± 6,07 e 63,52 ± 2,12 mg L-1 CaCO3 , respectivamente, havendo diferença significativa entre estes resultados (P < 0,001.

  10. Hit discovery of Mycobacterium tuberculosis inosine 5'-monophosphate dehydrogenase, GuaB2, inhibitors.

    Science.gov (United States)

    Sahu, Niteshkumar U; Singh, Vinayak; Ferraris, Davide M; Rizzi, Menico; Kharkar, Prashant S

    2018-04-18

    Tuberculosis remains a global concern. There is an urgent need of newer antitubercular drugs due to the development of resistant forms of Mycobacterium tuberculosis (Mtb). Inosine 5'-monophosphate dehydrogenase (IMPDH), guaB2, of Mtb, required for guanine nucleotide biosynthesis, is an attractive target for drug development. In this study, we screened a focused library of 73 drug-like molecules with desirable calculated/predicted physicochemical properties, for growth inhibitory activity against drug-sensitive MtbH37Rv. The eight hits and mycophenolic acid, a prototype IMPDH inhibitor, were further evaluated for activity on purified Mtb-GuaB2 enzyme, target selectivity using a conditional knockdown mutant of guaB2 in Mtb, followed by cross-resistance to IMPDH inhibitor-resistant SRMV2.6 strain of Mtb, and activity on human IMPDH2 isoform. One of the hits, 13, a 5-amidophthalide derivative, has shown growth inhibitory potential and target specificity against the Mtb-GuaB2 enzyme. The hit, 13, is a promising molecule with potential for further development as an antitubercular agent. Copyright © 2018 Elsevier Ltd. All rights reserved.

  11. Potencial da economia de água potável pelo uso de água pluvial: análise de 40 cidades da Amazônia Potential for potable water savings by using rainwater: an analysis over 40 cities in Amazon

    Directory of Open Access Journals (Sweden)

    Jeferson Alberto de Lima

    2011-09-01

    Full Text Available A escassez de água é um problema cada vez mais severo em todo o mundo devido a fatores como o consumo excessivo de água bruta, as mudanas climáticas, a poluição da água e o consumo insustentável dos recursos hídricos. Sob essas condições, formas tradicionais ou alternativas de recursos hídricos, tais como a água pluvial, estão sendo consideradas como opções atrativas para reduzir o consumo de água potável. Neste contexto, este artigo descreve o cenário de disponibilidade de água na região Amazõnica, Noroeste do Brasil, e avalia o potencial da economia de água potável para o setor residencial em 40 cidades da região. Os resultados indicam que o potencial da economia de água potável varia entre 21 e 100%, dependendo da demanda de água potável verificada nas 40 cidades, com potencial médio de 76%. A prisncipal conclusão desta pesquisa é que, se houvesse um programa do governo para promover a economia de água potável por meio da utilização da água pluvial, haveria significativa economia de água potável e, consequentemente, a preservação dos recursos hídricos na Amazõnia.Water scarcity is an increasingly severe problem worldwide due to some factors, such as the excessive consumption of raw water, climate changes, water pollution, and unsustainable water resource consumption. Under these conditions, traditional or alternative forms of water resource, such as rainwater, are being considered as attractive options to reduce potable water consumption. In this context, this paper describes the water availability scenario in the Amazon region, Brazilian Northeast, and it evaluates the potential for potable water savings estimated for the residential sector of 40 cities in the region. Results indicate that the potential for potable water savings range from 21 to 100% depending on the potable water demand verified in the 40 cities, with an average potential for potable water savings of 76%. The main conclusion is

  12. Promoter polymorphisms in genes involved in porcine myogenesis influence their transcriptional activity.

    Science.gov (United States)

    Bongiorni, Silvia; Tilesi, Francesca; Bicorgna, Silvia; Iacoponi, Francesca; Willems, Daniela; Gargani, Maria; D'Andrea, MariaSilvia; Pilla, Fabio; Valentini, Alessio

    2014-11-07

    Success of meat production and selection for improvement of meat quality is among the primary aims in animal production. Meat quality traits are economically important in swine; however, the underlying genetic nature is very complex. Therefore, an improved pork production strongly depends on identifying and studying how genetic variations contribute to modulate gene expression. Promoters are key regions in gene modulation as they harbour several binding motifs to transcription regulatory factors. Therefore, polymorphisms in these regions are likely to deeply affect RNA levels and consequently protein synthesis. In this study, we report the identification of single nucleotide polymorphisms (SNPs) in promoter regions of candidate genes involved in development, cellular differentiation and muscle growth in Sus scrofa. We identified SNPs in the promoter regions of genes belonging to the Myogenic Regulatory Factors (MRF) gene family (the Myogenic Differentiation gene, MYOD1) and to Growth and Differentiation Factors (GDF) gene family (Myostatin gene, MSTN, GDF8), in Casertana and Large White breeds. The purpose of this study was to investigate if polymorphisms in the promoters could affect the transcriptional activity of these genes. With this aim, we evaluated in vitro the functional activity of the luciferase reporter gene luc2 activity, driven by two constructs carrying different promoter haplotypes. We tested the effects of the G302A (U12574) transition on the promoter efficiency in MYOD1 gene. We ascertained a difference in transcription efficiency for the two variants. A stronger activity of the A-carrying construct is more evident in C2C12. The luciferase expression driven by the MYOD1-A allelic variant displayed a 3.8-fold increased transcriptional activity. We investigated the activity of two haplotype variants (AY527152) in the promoter of GDF8 gene. The haploptype-1 (A435-A447-A879) up-regulated the expression of the reporter gene by a two-fold increase, and

  13. Genes involved in immunity and apoptosis are associated with human presbycusis based on microarray analysis.

    Science.gov (United States)

    Dong, Yang; Li, Ming; Liu, Puzhao; Song, Haiyan; Zhao, Yuping; Shi, Jianrong

    2014-06-01

    Genes involved in immunity and apoptosis were associated with human presbycusis. CCR3 and GILZ played an important role in the pathogenesis of presbycusis, probably through regulating chemokine receptor, T-cell apoptosis, or T-cell activation pathways. To identify genes associated with human presbycusis and explore the molecular mechanism of presbycusis. Hearing function was tested by pure-tone audiometry. Microarray analysis was performed to identify presbycusis-correlated genes by Illumina Human-6 BeadChip using the peripheral blood samples of subjects. To identify biological process categories and pathways associated with presbycusis-correlated genes, bioinformatics analysis was carried out by Gene Ontology Tree Machine (GOTM) and database for annotation, visualization, and integrated discovery (DAVID). Quantitative RT-PCR (qRT-PCR) was used to validate the microarray data. Microarray analysis identified 469 up-regulated genes and 323 down-regulated genes. Both the dominant biological processes by Gene Ontology (GO) analysis and the enriched pathways by Kyoto encyclopedia of genes and genomes (KEGG) and BIOCARTA showed that genes involved in immunity and apoptosis were associated with presbycusis. In addition, CCR3, GILZ, CXCL10, and CX3CR1 genes showed consistent difference between groups for both the gene chip and qRT-PCR data. The differences of CCR3 and GILZ between presbycusis patients and controls were statistically significant (p < 0.05).

  14. Reservatório de ardósia para sistemas de aproveitamento de água de chuva

    Directory of Open Access Journals (Sweden)

    Marcio Antonio Nogueira Andrade

    Full Text Available RESUMO No contexto da crescente pressão exercida no mundo todo sobre os recursos hídricos, qualitativa e quantitativamente, o desenvolvimento de tecnologias apropriadas para sistemas de aproveitamento de água de chuva surge como alternativa promissora. Este trabalho tem como principal objetivo apresentar uma tecnologia inovadora para armazenamento de água de chuva, trazendo como modelo a construção de um reservatório executado com placas de ardósia, com capacidade para 250 m3, que faz parte de um sistema de aproveitamento de água de chuva implantado em uma instalação suinícola no Oeste Catarinense. Nesse sistema, a água de chuva é destinada para dessedentação animal e higienização das instalações. Também são apresentados, porém com menos enfoque, os dispositivos de tratamento da água de chuva desenvolvidos no projeto. Realizou-se uma análise comparativa de custos entre esse reservatório e os comumente utilizados na região de estudo. Também foram feitas análises físico-químicas e bacteriológicas da água de chuva em diferentes pontos do sistema, a fim de verificar se a qualidade da água é adequada para os usos pretendidos. A tecnologia de construção de reservatórios de ardósia apresentou rapidez e facilidade de execução e baixo custo de construção. A ardósia, além de servir de forma para a moldagem da armadura e para a argamassagem, é um material natural e abundante na região central do Estado de Santa Catarina. As análises físico-químicas e bacteriológicas mostraram a eficiência dos dispositivos de tratamento da água de chuva aplicados neste trabalho. Por fim, observou-se que a água de chuva é adequada para dessedentação animal, já que atende às condições e padrões para as águas de Classe 3 da Resolução CONAMA 357. Deste modo, a nova tecnologia apresenta grande potencial de aplicação e viabilidade econômica para a região de estudo.

  15. Modelagem da curva de retenção de água de Latossolos utilizando a Equação Duplo Van Genuchten

    Directory of Open Access Journals (Sweden)

    Carla Eloize Carducci

    2011-02-01

    Full Text Available Os Latossolos da região do Cerrado brasileiro, em função da mineralogia da fração argila e estrutura granular, apresentam elevado volume de poros grandes e de poros extremamente pequenos, não mostrando significativo volume de poros intermediários, o que faz com que neles a disponibilidade de água para as plantas seja baixa. Este trabalho objetivou caracterizar e modelar o comportamento da retenção de água em Latossolos oxídicos da região do Cerrado, pertencentes a diferentes classes texturais. Foram coletadas amostras do horizonte Bw de 10 Latossolos oxídicos sob vegetação nativa. A retenção de água nos potenciais matriciais de 1, 2, 4, 6, 8 e 10 kPa foi obtida em unidade de sucção nos potenciais de 33, 60, 100, 500 e 1.500 kPa no extrator de Richards, e a água retida sob potenciais de 1.500 a 300.000 kPa foi quantificada utilizando o psicrômetro de termopar WP4-T. O modelo duplo van Genuchten foi proposto para ajustar os dados experimentais de retenção de água por meio de procedimentos de ajuste de modelos não lineares do software R 2.10.1; também foi avaliada a relação entre as estimativas dos parâmetros do modelo, bem como a inclinação do ponto de inflexão com as propriedades texturais dos solos, aplicando o teste de correlação de Pearson. Os resultados mostraram o bom ajuste do modelo e alto poder de predição, sendo observada correlação do conteúdo de argila do solo com os parâmetros da equação (Usat, Upmp, Ures, assim como, com a inclinação do segundo ponto de inflexão (Itex. A textura dos Latossolos influenciou o comportamento das curvas de retenção de água. As curvas de retenção de água dos Latossolos em estudo apresentaram comportamento bimodal no intervalo de potencial matricial estudado.

  16. Dicer and Argonaute Genes Involved in RNA Interference in the Entomopathogenic Fungus Metarhizium robertsii.

    Science.gov (United States)

    Meng, Huimin; Wang, Zhangxun; Wang, Yulong; Zhu, Hong; Huang, Bo

    2017-04-01

    RNA interference (RNAi) is a gene-silencing mechanism that plays an important role in gene regulation in a number of eukaryotic organisms. Two core components, Dicer and Argonaute, are central in the RNAi machinery. However, the physiological roles of Dicer and Argonaute in the entomopathogenic fungus Metarhizium robertsii have remained unclear. Here, the roles of genes encoding Dicer ( M. robertsii dcl1 [ Mrdcl1 ] and Mrdcl2 ) and Argonaute ( Mrago1 and Mrago2 ) proteins in M. robertsii were investigated. The results showed that the Dicer-like protein MrDCL2 and Argonaute protein MrAGO1 are the major components of the RNAi process occurring in M. robertsii The Dicer and Argonaute genes were not involved in the regulation of growth and diverse abiotic stress response in M. robertsii under the tested conditions. Moreover, our results showed that the Dicer and Argonaute gene mutants demonstrated reduced abilities to produce conidia, compared to the wild type (WT) and the gene-rescued mutant. In particular, the conidial yields in the Δ dcl2 and Δ ago1 mutants were reduced by 55.8% and 59.3%, respectively, compared with those from the control strains. Subsequently, for the WT and Δ dcl2 mutant strains, digital gene expression (DGE) profiling analysis of the stage of mycelium growth and conidiogenesis revealed that modest changes occur in development or metabolism processes, which may explain the reduction in conidiation in the Δ dcl2 mutant. In addition, we further applied high-throughput sequencing technology to identify small RNAs (sRNAs) that are differentially expressed in the WT and the Δ dcl2 mutant and found that 4 known microRNA-like small RNAs (milRNAs) and 8 novel milRNAs were Mrdcl2 dependent in M. robertsii IMPORTANCE The identification and characterization of components in RNAi have contributed significantly to our understanding of the mechanism and functions of RNAi in eukaryotes. Here, we found that Dicer and Argonaute genes play an important role

  17. Integrative analysis for finding genes and networks involved in diabetes and other complex diseases

    DEFF Research Database (Denmark)

    Bergholdt, R.; Størling, Zenia, Marian; Hansen, Kasper Lage

    2007-01-01

    We have developed an integrative analysis method combining genetic interactions, identified using type 1 diabetes genome scan data, and a high-confidence human protein interaction network. Resulting networks were ranked by the significance of the enrichment of proteins from interacting regions. We...... identified a number of new protein network modules and novel candidate genes/proteins for type 1 diabetes. We propose this type of integrative analysis as a general method for the elucidation of genes and networks involved in diabetes and other complex diseases....

  18. Identification and characterization of nuclear genes involved in photosynthesis in Populus

    Science.gov (United States)

    2014-01-01

    Background The gap between the real and potential photosynthetic rate under field conditions suggests that photosynthesis could potentially be improved. Nuclear genes provide possible targets for improving photosynthetic efficiency. Hence, genome-wide identification and characterization of the nuclear genes affecting photosynthetic traits in woody plants would provide key insights on genetic regulation of photosynthesis and identify candidate processes for improvement of photosynthesis. Results Using microarray and bulked segregant analysis strategies, we identified differentially expressed nuclear genes for photosynthesis traits in a segregating population of poplar. We identified 515 differentially expressed genes in this population (FC ≥ 2 or FC ≤ 0.5, P photosynthesis by the nuclear genome mainly involves transport, metabolism and response to stimulus functions. Conclusions This study provides new genome-scale strategies for the discovery of potential candidate genes affecting photosynthesis in Populus, and for identification of the functions of genes involved in regulation of photosynthesis. This work also suggests that improving photosynthetic efficiency under field conditions will require the consideration of multiple factors, such as stress responses. PMID:24673936

  19. The genomic structure of the DMBT1 gene

    DEFF Research Database (Denmark)

    Mollenhauer, J; Holmskov, U; Wiemann, S

    1999-01-01

    Increasing evidence has accumulated for an involvement of the inactivation of tumour suppressor genes at chromosome 10q in the carcinogenesis of brain tumours, melanomas, and carcinomas of the lung, the prostate, the pancreas, and the endometrium. The gene DMBT1 (Deleted in Malignant Brain Tumours...... 1) is located at chromosome 10q25.3-q26.1, within one of the putative intervals for tumour suppressor genes. DMBT1 is a member of the scavenger-receptor cysteine-rich (SRCR) superfamily and displays homozygous deletions or lack of expression in glioblastoma multiforme, medulloblastoma......, and in gastrointestinal and lung cancers. Based on these properties, DMBT1 has been proposed to be a candidate tumour suppressor gene. We have determined the genomic sequence of DMBT1 to allow analyses of mutations. The gene has at least 54 exons that span a genomic region of about 80 kb. We have identified a putative...

  20. Identification of new genes involved in human adipogenesis and fat storage.

    Directory of Open Access Journals (Sweden)

    Jörn Söhle

    Full Text Available Since the worldwide increase in obesity represents a growing challenge for health care systems, new approaches are needed to effectively treat obesity and its associated diseases. One prerequisite for advances in this field is the identification of genes involved in adipogenesis and/or lipid storage. To provide a systematic analysis of genes that regulate adipose tissue biology and to establish a target-oriented compound screening, we performed a high throughput siRNA screen with primary (preadipocytes, using a druggable siRNA library targeting 7,784 human genes. The primary screen showed that 459 genes affected adipogenesis and/or lipid accumulation after knock-down. Out of these hits, 333 could be validated in a secondary screen using independent siRNAs and 110 genes were further regulated on the gene expression level during adipogenesis. Assuming that these genes are involved in neutral lipid storage and/or adipocyte differentiation, we performed InCell-Western analysis for the most striking hits to distinguish between the two phenotypes. Beside well known regulators of adipogenesis and neutral lipid storage (i.e. PPARγ, RXR, Perilipin A the screening revealed a large number of genes which have not been previously described in the context of fatty tissue biology such as axonemal dyneins. Five out of ten axonemal dyneins were identified in our screen and quantitative RT-PCR-analysis revealed that these genes are expressed in preadipocytes and/or maturing adipocytes. Finally, to show that the genes identified in our screen are per se druggable we performed a proof of principle experiment using an antagonist for HTR2B. The results showed a very similar phenotype compared to knock-down experiments proofing the "druggability". Thus, we identified new adipogenesis-associated genes and those involved in neutral lipid storage. Moreover, by using a druggable siRNA library the screen data provides a very attractive starting point to identify anti

  1. Cloning of the rat Waf1/Cip1 gene

    International Nuclear Information System (INIS)

    Belinsky, S.A.; Middleton, S.K.

    1994-01-01

    The progression of eukaryotic cells through the cell cycle involves the sequential expression of specific genes. This process is regulated by both external and internal stimuli that prevent the cell from prematurely entering the next phase before all macromolecular events have been completed. The activation and subsequent inactivation of cyclin dependent kinases (Cdks) represent one internal stimuli required to regulate the transit of cells from one stage of the cell cycle to the next. Another member of this regulatory cascade is the p53 tumor suppressor gene, which controls a G 1 checkpoint at which the cell cycle can be arrested prior to the initiation of DNA synthesis. Following DNA damage, p53 protein levels rise, and entry into S phase is delayed, presumably to allow time for repair of the lesions. When p53 function is lost, cells containing damaged DNA template enter S phase leading to fixation and propagation of genetic alterations. Recently, evidence linking the growth-suppressing activity of p53 and inactivation of Cdks has been provided by the cloning of the Waf1/Cip1 gene. Waf1/Cip1 encodes a protein of M r 21,000 (p21), which inhibits Cdks in vitro. The overexpression of Waf1/Cip1 in cells inhibits cell growth, suggesting that p21 is a downstream mediator of p53 function. Loss of Waf1/Cip1 gene function could lead to deregulation of the cell cycle and contribute to the development of the neoplastic phenotype in tumors that do not contain mutations in the p53 gene. The purpose of the present investigation was to clone the rat Waf1/Cip1 gene,then determine the frequency for alteration of this gene in lung tumors induced by X-rays

  2. Desinfecção de água cinza por fotocatálise heterogênea

    Directory of Open Access Journals (Sweden)

    Anderson Teodoro

    2017-07-01

    Full Text Available RESUMO A desinfecção adequada de águas cinzas é necessária para garantir a segurança de sua reutilização, principalmente em aplicações com potencial de exposição humana. Diversos processos oxidativos avançados têm sido empregados nos últimos anos para a degradação de contaminantes orgânicos, bem como para desinfecção de águas e efluentes. O objetivo deste trabalho foi testar TiO2 suportado em microtubos para desinfetar águas cinzas por meio da fotocatálise heterogênea, visando ao reúso hídrico em bacia sanitária. A água cinza utilizada nos experimentos foi coletada após passar por um sistema de tratamento composto de um tanque de evapotranspiração seguido de banhado construído de fluxo horizontal. Foram realizados testes em batelada utilizando-se um reator fotoquímico cilíndrico de 1,0 L (volume total do reator, preenchido com pequenos cilindros de vidro do tipo Pyrex com TiO2 suportado. Para os testes de desinfecção, foram utilizados os processos UV, H2O2, UV/TiO2, UV/H2O2 e UV/TiO2/H2O2. Foi possível obter uma camada homogênea de TiO2 depositada nos pequenos tubos de vidro Pyrex, com espessura média de 35,3 µm, capaz de promover um incremento na desinfecção de águas cinzas. Porém, mesmo com um maior poder de desinfecção do TiO2 - se comparado com a fotólise (UV -, os processos em que se empregou o peróxido de hidrogênio foram bem mais eficientes, tanto na desinfecção (inativação total de coliformes totais, enterococos e Pseudomonas aeruginosa quanto na remoção de matéria orgânica em termos de demanda química de oxigênio (em torno de 60%. As amostras mantidas em temperatura ambiente e envolvidas por plástico escuro não apresentaram recrescimento bacteriano com 24h de armazenamento após os experimentos, mostrando assim a viabilidade da água cinza tratada em reúso doméstico.

  3. Caenorhabditis elegans expressing the Saccharomyces cerevisiae NADH alternative dehydrogenase Ndi1p, as a tool to identify new genes involved in complex I related diseases

    Directory of Open Access Journals (Sweden)

    Raynald eCossard

    2015-06-01

    Full Text Available Isolated complex I deficiencies are one of the most commonly observed biochemical features in patients suffering from mitochondrial disorders. In the majority of these clinical cases the molecular bases of the diseases remain unknown suggesting the involvement of unidentified factors that are critical for complex I function.The Saccharomyces cerevisiae NDI1 gene, encoding the mitochondrial internal NADH dehydrogenase was previously shown to complement a complex I deficient strain in Caenorhabitis elegans with notable improvements in reproduction, whole organism respiration. These features indicate that Ndi1p can functionally integrate the respiratory chain, allowing complex I deficiency complementation. Taking into account the Ndi1p ability to bypass complex I, we evaluate the possibility to extend the range of defects/mutations causing complex I deficiencies that can be alleviated by NDI1 expression.We report here that NDI1 expressing animals unexpectedly exhibit a slightly shortened lifespan, a reduction in the progeny and a depletion of the mitochondrial genome. However, Ndi1p is expressed and targeted to the mitochondria as a functional protein that confers rotenone resistance to those animals and without affecting their respiration rate and ATP content.We show that the severe embryonic lethality level caused by the RNAi knockdowns of complex I structural subunit encoding genes (e.g. NDUFV1, NDUFS1, NDUFS6, NDUFS8 or GRIM-19 human orthologs in wild type animals is significantly reduced in the Ndi1p expressing worm.All together these results open up the perspective to identify new genes involved in complex I function, assembly or regulation by screening an RNAi library of genes leading to embryonic lethality that should be rescued by NDI1 expression.

  4. ESKIMO1 is a key gene involved in water economy as well as cold acclimation and salt tolerance

    DEFF Research Database (Denmark)

    Bouchabke-Coussa, O.; Quashie, M.L.; Seoane, Jose Miguel

    2008-01-01

    's improved tolerance to reduced water supply may be explained by its lower transpiration rate and better water use efficiency (WUE), which was assessed by carbon isotope discrimination and gas exchange measurements. esk1 alleles were also shown to be more tolerant to salt stress. Transcriptomic analysis......Background: Drought is a major social and economic problem resulting in huge yield reduction in the field. Today's challenge is to develop plants with reduced water requirements and stable yields in fluctuating environmental conditions. Arabidopsis thaliana is an excellent model for identifying...... as a key gene involved in plant water economy as well as cold acclimation and salt tolerance. Results: All esk1 mutants were more tolerant to freezing, after acclimation, than their wild type counterpart. esk1 mutants also showed increased tolerance to mild water deficit for all traits measured. The mutant...

  5. Mensuração do potencial erosivo de balas dissolvidas em água e saliva artificial

    Directory of Open Access Journals (Sweden)

    Brenda BONVINI

    Full Text Available Resumo Introdução O consumo excessivo e frequente de balas ácidas pode estar associado à etiologia da erosão dental. Objetivo Mensurar e comparar o pH e a acidez titulável de balas dissolvidas em água e saliva artificial. Material e método Foram utilizadas as balas Tic Tac® sabores laranja, cereja/maracujá e morango, agrupadas em dois grupos: G-1: balas dissolvidas em água; G-2: balas dissolvidas em saliva artificial. As balas foram trituradas e, do pó resultante de cada sabor, foram pesadas duas amostras de 20 g, sendo então dissolvidas em 120 mL de água destilada ou 120 mL de saliva artificial. Destas soluções, obtiveram-se três amostras de 30 mL para cada um dos sabores, permitindo a leitura em triplicata do pH e da acidez titulável. O pH foi mensurado utilizando-se um potenciômetro e eletrodo combinado de vidro, previamente calibrado com soluções padrão pH 7,0 e pH 4,0. Para a verificação da acidez titulável, adicionaram-se alíquotas de 100 μL NaOH 1M, até o pH alcançar 5,5. Submeteram-se os resultados à Análise de Variância (ANOVA. As comparações das médias foram realizadas pelo Teste Tukey, em um nível de 5% de significância (p<0,05. Resultado Todas as balas apresentaram pH abaixo do crítico para dissolução do esmalte, quando dissolvidas em água e saliva artificial. Na comparação entre os grupos, o G-2, mostrou um pH mais elevado e menor acidez titulável, diferindo significantemente do G-1. Conclusão Todas as balas dissolvidas em água e saliva artificial mostraram-se potencialmente erosivas, podendo contribuir para a etiologia da erosão dental.

  6. Comportamento do alumínio num sistema público de água para consumo humano

    OpenAIRE

    Nunes, Marisa de Lurdes Pedrosa Venâncio

    2010-01-01

    Por forma a satisfazer os requisitos regulamentares e as necessidades dos consumidores, as entidades gestoras de sistemas de abastecimento de água têm necessidade de compreender melhor os movimentos e transformações a que a água, destinada ao consumo humano, está sujeita ao longo dos sistemas de distribuição, de modo a proteger a sua qualidade em sistemas públicos de abastecimento. Os modelos de simulação para sistemas de transporte e distribuição de água constituem instr...

  7. The evolutionary history of the SAL1 gene family in eutherian mammals

    Directory of Open Access Journals (Sweden)

    Callebaut Isabelle

    2011-05-01

    Full Text Available Abstract Background SAL1 (salivary lipocalin is a member of the OBP (Odorant Binding Protein family and is involved in chemical sexual communication in pig. SAL1 and its relatives may be involved in pheromone and olfactory receptor binding and in pre-mating behaviour. The evolutionary history and the selective pressures acting on SAL1 and its orthologous genes have not yet been exhaustively described. The aim of the present work was to study the evolution of these genes, to elucidate the role of selective pressures in their evolution and the consequences for their functions. Results Here, we present the evolutionary history of SAL1 gene and its orthologous genes in mammals. We found that (1 SAL1 and its related genes arose in eutherian mammals with lineage-specific duplications in rodents, horse and cow and are lost in human, mouse lemur, bushbaby and orangutan, (2 the evolution of duplicated genes of horse, rat, mouse and guinea pig is driven by concerted evolution with extensive gene conversion events in mouse and guinea pig and by positive selection mainly acting on paralogous genes in horse and guinea pig, (3 positive selection was detected for amino acids involved in pheromone binding and amino acids putatively involved in olfactory receptor binding, (4 positive selection was also found for lineage, indicating a species-specific strategy for amino acid selection. Conclusions This work provides new insights into the evolutionary history of SAL1 and its orthologs. On one hand, some genes are subject to concerted evolution and to an increase in dosage, suggesting the need for homogeneity of sequence and function in certain species. On the other hand, positive selection plays a role in the diversification of the functions of the family and in lineage, suggesting adaptive evolution, with possible consequences for speciation and for the reinforcement of prezygotic barriers.

  8. PREDIÇÃO DA ATIVIDADE DE ÁGUA E RELAÇÃO ENTRE A ATIVIDADE DE ÁGUA E A DEPRESSÃO DO PONTO DE CONGELAMENTO DE SUCOS DE FRUTAS

    Directory of Open Access Journals (Sweden)

    MOURA Silvia Cristina Sobottka Rolim de

    1998-01-01

    Full Text Available O presente trabalho se propôs a determinar a atividade de água e a depressão do ponto de congelamento dos suco de tangerina, abacaxi e limão a várias concentrações (10-55oBrix e obter uma correlação entre as duas propriedades. A depressão do ponto de congelamento é determinada experimentalmente com auxílio do crioscópio de marca LAKTRON e de equipamentos comuns de laboratório. A atividade de água foi determinada pelo higrômetro marca DECAGON CX-2 na faixa de temperaturas de 15 a 30oC. Com os resultados obtidos, verificou-se o ajuste à equação para predição de atividade de água de CHEN (1987 para misturas de não eletrólitos, através do cálculo do coeficiente da variação do ajuste (CV. Sendo este menor que 3% para o modelo proposto, pode-se dizer que os dados experimentais se ajustaram bem à equação de predição. A atividade de água e a depressão do ponto de congelamento são correlacionadas, para os sucos de tangerina, abacaxi e limão com valor de r2 maior que 96% sendo, portanto, possível obter a atividade de água através do conhecimento da depressão do ponto de congelamento dos sucos estudados.

  9. ÁGUA E SEUS INSTRUMENTOS LEGAIS DE PROTEÇÃO / WATER AND YOUR PROTECTION LEGAL INSTRUMENTS

    Directory of Open Access Journals (Sweden)

    U. G. F. Tamarindo

    2015-03-01

    Full Text Available O Brasil é naturalmente privilegiado com grandes rios e vegetações em seu território, todavia, ao longo das últimas décadas, o seu maior patrimônio, a água doce, está sendo seriamente comprometido. Nessa ordem, é fato que a água nunca foi muito respeitada, talvez por ser gratuita e abundante. Entretanto, a situação atual de escassez de água em muitas regiões do País somada ao mau uso da terra e demais recursos naturais têm produzido, além de fortes alterações climáticas, importantes mudanças na economia e na sociedade em geral. A grave seca hoje vivenciada principalmente no Sudeste e Centro-Oeste do Brasil, bem como o excesso de chuvas e enchentes em outras partes do País deixa evidente tal advertência. O Brasil está vulnerável a todas essas alterações climáticas. E em relação à água, se não houver mudanças concretas no uso o resultado em pouco tempo será catastrófico para todos. Não se pode conceber a permanência desse estado atual de inércia ambiental em relação às águas. As gerações atuais têm a obrigação de conhecer e, sobretudo colocar em prática as tutelas legais de proteção aos ecossistemas, notadamente das águas. E como afirmam Maude Barlow e Tony Clarke, “se não mudarmos logo nossa relação com a água e com os ecossistemas que a mantêm, toda a nossa riqueza e conhecimento não terão sentido algum”. A legislação nacional e internacional estabelecem meios de proteção à água, notadamente sob a perspectiva de que esta pode ser sujeito de direitos. Este artigo, portanto, apresenta uma visão geral das tutelas legais de proteção à água.

  10. Determinação de coliformes na água e no tecido mole das ostras extraídas em Cananéia, São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Sonia Assami Doi

    2015-03-01

    Full Text Available Este estudo objetivou avaliar a qualidade microbiológica da água de várias localidades em que são produzidas ostras Crassostrea sp. no estuário de Cananéia, tanto como nos bivalves, por meio dos valores de coliformes baseados na Técnica dos Tubos Múltiplos. Foram realizadas coletas de água em dez localidades durante os anos de 2005 a 2011. A análise da água apresentou média geométrica de coliformes termotolerantes de 24,67 NMP 100 mL-1 e, nos tecidos das ostras, de 15,53 NMP g-1. Verificou-se uma diferença significativa entre as localidades analisadas quanto à densidade de coliformes totais e termotolerantes na água. Analisaram-se as variações das contagens bacterianas em relação à sazonalidade, verificando-se que o outono apresentou a maior média de coliformes nas ostras, sendo os maiores dados coletados em abril. Houve correlação positiva entre coliformes na água e na ostra.

  11. Growth rate regulated genes and their wide involvement in the Lactococcus lactis stress responses

    Directory of Open Access Journals (Sweden)

    Redon Emma

    2008-07-01

    Full Text Available Abstract Background The development of transcriptomic tools has allowed exhaustive description of stress responses. These responses always superimpose a general response associated to growth rate decrease and a specific one corresponding to the stress. The exclusive growth rate response can be achieved through chemostat cultivation, enabling all parameters to remain constant except the growth rate. Results We analysed metabolic and transcriptomic responses of Lactococcus lactis in continuous cultures at different growth rates ranging from 0.09 to 0.47 h-1. Growth rate was conditioned by isoleucine supply. Although carbon metabolism was constant and homolactic, a widespread transcriptomic response involving 30% of the genome was observed. The expression of genes encoding physiological functions associated with biogenesis increased with growth rate (transcription, translation, fatty acid and phospholipids metabolism. Many phages, prophages and transposon related genes were down regulated as growth rate increased. The growth rate response was compared to carbon and amino-acid starvation transcriptomic responses, revealing constant and significant involvement of growth rate regulations in these two stressful conditions (overlap 27%. Two regulators potentially involved in the growth rate regulations, llrE and yabB, have been identified. Moreover it was established that genes positively regulated by growth rate are preferentially located in the vicinity of replication origin while those negatively regulated are mainly encountered at the opposite, thus indicating the relationship between genes expression and their location on chromosome. Although stringent response mechanism is considered as the one governing growth deceleration in bacteria, the rigorous comparison of the two transcriptomic responses clearly indicated the mechanisms are distinct. Conclusion This work of integrative biology was performed at the global level using transcriptomic analysis

  12. Global Identification of EVI1 Target Genes in Acute Myeloid Leukemia.

    Directory of Open Access Journals (Sweden)

    Carolyn Glass

    Full Text Available The ecotropic virus integration site 1 (EVI1 transcription factor is associated with human myeloid malignancy of poor prognosis and is overexpressed in 8-10% of adult AML and strikingly up to 27% of pediatric MLL-rearranged leukemias. For the first time, we report comprehensive genomewide EVI1 binding and whole transcriptome gene deregulation in leukemic cells using a combination of ChIP-Seq and RNA-Seq expression profiling. We found disruption of terminal myeloid differentiation and cell cycle regulation to be prominent in EVI-induced leukemogenesis. Specifically, we identified EVI1 directly binds to and downregulates the master myeloid differentiation gene Cebpe and several of its downstream gene targets critical for terminal myeloid differentiation. We also found EVI1 binds to and downregulates Serpinb2 as well as numerous genes involved in the Jak-Stat signaling pathway. Finally, we identified decreased expression of several ATP-dependent P2X purinoreceptors genes involved in apoptosis mechanisms. These findings provide a foundation for future study of potential therapeutic gene targets for EVI1-induced leukemia.

  13. CARACTERIZAÇÃO E TRATAMENTO DE ÁGUAS CINZAS VISANDO REÚSO DOMÉSTICO

    OpenAIRE

    Geraldo Rampelotto

    2014-01-01

    A produção e disseminação do conhecimento para as questões ambientais, considerando os aspectos sociais e econômicos, entre eles, o reúso de águas, aplicado para fins não potáveis vem ao encontro das premissas de planeta sustentável. As águas cinzas, quando devidamente tratadas, podem ser utilizadas para consumo não potável em edificações como, por exemplo, descarga de vasos sanitários, lavagem de pisos, lavagem de calçadas, irrigação de jardins, usos ornamentais como espelhos dágua e chafari...

  14. Desinfecção de água cinza por fotocatálise heterogênea

    OpenAIRE

    Anderson Teodoro; Marc Árpad Boncz; Paula Loureiro Paulo; Amilcar Machulek Junior

    2017-01-01

    RESUMO A desinfecção adequada de águas cinzas é necessária para garantir a segurança de sua reutilização, principalmente em aplicações com potencial de exposição humana. Diversos processos oxidativos avançados têm sido empregados nos últimos anos para a degradação de contaminantes orgânicos, bem como para desinfecção de águas e efluentes. O objetivo deste trabalho foi testar TiO2 suportado em microtubos para desinfetar águas cinzas por meio da fotocatálise heterogênea, visando ao reúso hídric...

  15. Cryptochrome 1 regulates growth and development in Brassica through alteration in the expression of genes involved in light, phytohormone and stress signalling.

    Science.gov (United States)

    Sharma, Pooja; Chatterjee, Mithu; Burman, Naini; Khurana, Jitendra P

    2014-04-01

    The blue light photoreceptors cryptochromes are ubiquitous in higher plants and are vital for regulating plant growth and development. In spite of being involved in controlling agronomically important traits like plant height and flowering time, cryptochromes have not been extensively characterized from agriculturally important crops. Here we show that overexpression of CRY1 from Brassica napus (BnCRY1), an oilseed crop, results in short-statured Brassica transgenics, likely to be less prone to wind and water lodging. The overexpression of BnCRY1 accentuates the inhibition of cell elongation in hypocotyls of transgenic seedlings. The analysis of hypocotyl growth inhibition and anthocyanin accumulation responses in BnCRY1 overexpressors substantiates that regulation of seedling photomorphogenesis by cry1 is dependent on light intensity. This study highlights that the photoactivated cry1 acts through coordinated induction and suppression of specific downstream genes involved in phytohormone synthesis or signalling, and those involved in cell wall modification, during de-etiolation of Brassica seedlings. The microarray-based transcriptome profiling also suggests that the overexpression of BnCRY1 alters abiotic/biotic stress signalling pathways; the transgenic seedlings were apparently oversensitive to abscisic acid (ABA) and mannitol. © 2013 John Wiley & Sons Ltd.

  16. Banana ethylene response factors are involved in fruit ripening through their interactions with ethylene biosynthesis genes.

    Science.gov (United States)

    Xiao, Yun-yi; Chen, Jian-ye; Kuang, Jiang-fei; Shan, Wei; Xie, Hui; Jiang, Yue-ming; Lu, Wang-jin

    2013-05-01

    The involvement of ethylene response factor (ERF) transcription factor (TF) in the transcriptional regulation of ethylene biosynthesis genes during fruit ripening remains largely unclear. In this study, 15 ERF genes, designated as MaERF1-MaERF15, were isolated and characterized from banana fruit. These MaERFs were classified into seven of the 12 known ERF families. Subcellular localization showed that MaERF proteins of five different subfamilies preferentially localized to the nucleus. The 15 MaERF genes displayed differential expression patterns and levels in peel and pulp of banana fruit, in association with four different ripening treatments caused by natural, ethylene-induced, 1-methylcyclopropene (1-MCP)-delayed, and combined 1-MCP and ethylene treatments. MaERF9 was upregulated while MaERF11 was downregulated in peel and pulp of banana fruit during ripening or after treatment with ethylene. Furthermore, yeast-one hybrid (Y1H) and transient expression assays showed that the potential repressor MaERF11 bound to MaACS1 and MaACO1 promoters to suppress their activities and that MaERF9 activated MaACO1 promoter activity. Interestingly, protein-protein interaction analysis revealed that MaERF9 and -11 physically interacted with MaACO1. Taken together, these results suggest that MaERFs are involved in banana fruit ripening via transcriptional regulation of or interaction with ethylene biosynthesis genes.

  17. Involvement of Multiple Gene-Silencing Pathways in a Paramutation-like Phenomenon in Arabidopsis

    Directory of Open Access Journals (Sweden)

    Zhimin Zheng

    2015-05-01

    Full Text Available Paramutation is an epigenetic phenomenon that has been observed in a number of multicellular organisms. The epigenetically silenced state of paramutated alleles is not only meiotically stable but also “infectious” to active homologous alleles. The molecular mechanism of paramutation remains unclear, but components involved in RNA-directed DNA methylation (RdDM are required. Here, we report a multi-copy pRD29A-LUC transgene in Arabidopsis thaliana that behaves like a paramutation locus. The silent state of LUC is induced by mutations in the DNA glycosylase gene ROS1. The silent alleles of LUC are not only meiotically stable but also able to transform active LUC alleles into silent ones, in the absence of ros1 mutations. Maintaining silencing at the LUC gene requires action of multiple pathways besides RdDM. Our study identified specific factors that are involved in the paramutation-like phenomenon and established a model system for the study of paramutation in Arabidopsis.

  18. The light gene of Drosophila melanogaster encodes a homologue of VPS41, a yeast gene involved in cellular-protein trafficking.

    Science.gov (United States)

    Warner, T S; Sinclair, D A; Fitzpatrick, K A; Singh, M; Devlin, R H; Honda, B M

    1998-04-01

    Mutations in a number of genes affect eye colour in Drosophila melanogaster; some of these "eye-colour" genes have been shown to be involved in various aspects of cellular transport processes. In addition, combinations of viable mutant alleles of some of these genes, such as carnation (car) combined with either light (lt) or deep-orange (dor) mutants, show lethal interactions. Recently, dor was shown to be homologous to the yeast gene PEP3 (VPS18), which is known to be involved in intracellular trafficking. We have undertaken to extend our earlier work on the lt gene, in order to examine in more detail its expression pattern and to characterize its gene product via sequencing of a cloned cDNA. The gene appears to be expressed at relatively high levels in all stages and tissues examined, and shows strong homology to VPS41, a gene involved in cellular-protein trafficking in yeast and higher eukaryotes. Further genetic experiments also point to a role for lt in transport processes: we describe lethal interactions between viable alleles of lt and dor, as well as phenotypic interactions (reductions in eye pigment) between allels of lt and another eye-colour gene, garnet (g), whose gene product has close homology to a subunit of the human adaptor complex, AP-3.

  19. ISOLAMENTO E IDENTIFICAÇÃO DE MICOBACTÉRIAS EM ÁGUAS TRATADAS PROVENIENTES DO SISTEMA DE ABASTECIMENTO DE ARARAQUARA-SP

    Directory of Open Access Journals (Sweden)

    Josiane Aparecida GASPAR-GRILLO

    2012-01-01

    Full Text Available A qualidade da água é muito importante para a saúde e bem-estar do ser humano e o sistema de abastecimento público deve fornecer água de qualidade e em quantidade suficiente para toda a população. As estações de tratamento de água constituem o principal caminho para obtenção de água de qualidade. Quando isso não ocorre vários problemas podem afetar a população que passa a consumir água com qualidade inadequada e com o risco constante de surgimento de várias doenças. A eliminação de micro-organismos em água tratada reduz a competição, favorecendo a multiplicação de bactérias resistentes ao cloro como as do gênero Mycobacterium frequentemente isoladas de águas tratadas e cloradas. Considerando a não indicação da pesquisa de micobactérias nos exames laboratoriais de rotina para controle de qualidade de água para consumo humano e outros usos, o objetivo deste trabalho foi verificar a presença, isolar e identificar as micobactérias ambientais no sistema de abastecimento de água de origem superficial da cidade de Araraquara – SP. Foram analisadas 40 amostras de águas, assim distribuídas: dez de água bruta colhidas na Estação de Tratamento de Água (ETA, dez colhidas após filtração, dez colhidas no reservatório após cloração e dez na rede de distribuição. Foram recuperados 43 isolados de micobactérias. Todos os isolados foram submetidos ao PCR-PRA. As espécies de micobactérias identificadas foram M. lentiflavum, M. parafortuitum, M. genavense, M. gordonae, M. fortuitum, M. confluentis, M. duvalii, M. avium subespécie paratuberculose e M. szulgai. Com esses resultados, concluiu-se que a água é importante fonte de micobactérias ambientais provavelmente relacionadas a várias doenças humanas, sugerindo-se a realização de acompanhamento contínuo desses micro-organismos no sistema de água potável.

  20. The Pic19 NBS-LRR gene family members are closely linked to Scmv1, but not involved in maize resistance to sugarcane mosaic virus

    DEFF Research Database (Denmark)

    Jiang, Lu; Ingvardsen, Christina Rønn; Lübberstedt, Thomas

    2008-01-01

    the isolation and characterization of the Pic19R gene family members from the inbred line FAP1360A, which shows complete resistance to SCMV. Two primer pairs were designed based on the conserved regions among the known Pic19 paralogs and used for rapid amplification of cDNA ends of FAP1360A. Six full-length c...... of the Pic19R family indicated that the Pic19R-1 paralog is identical to the known Rxo1 gene conferring resistance to rice bacterial streak disease and none of the other Pic19R paralogs seems to be involved in resistance to SCMV...

  1. Isolation and characterization of the betalain biosynthesis gene involved in hypocotyl pigmentation of the allotetraploid Chenopodium quinoa.

    Science.gov (United States)

    Imamura, Tomohiro; Takagi, Hiroki; Miyazato, Akio; Ohki, Shinya; Mizukoshi, Hiroharu; Mori, Masashi

    2018-02-05

    In quinoa seedlings, the pigment betalain accumulates in the hypocotyl. To isolate the genes involved in betalain biosynthesis in the hypocotyl, we performed ethyl methanesulfonate (EMS) mutagenesis on the CQ127 variety of quinoa seedlings. While putative amaranthin and celosianin II primarily accumulate in the hypocotyls, this process produced a green hypocotyl mutant (ghy). This MutMap+ method using the quinoa draft genome revealed that the causative gene of the mutant is CqCYP76AD1-1. Our results indicated that the expression of CqCYP76AD1-1 was light-dependent. In addition, the transient expression of CqCYP76AD1-1 in Nicotiana benthamiana leaves resulted in the accumulation of betanin but not isobetanin, and the presence of a polymorphism in CqCYP76A1-2 in the CQ127 variety was shown to have resulted in its loss of function. These findings suggested that CqCYP76AD1-1 is involved in betalain biosynthesis during the hypocotyl pigmentation process in quinoa. To our knowledge, CqCYP76AD1-1 is the first quinoa gene identified by EMS mutagenesis using a draft gene sequence. Copyright © 2018. Published by Elsevier Inc.

  2. Study of the Genes and Mechanism Involved in the Radioadaptive Response

    Science.gov (United States)

    Dasgupta, Pushan R.

    2009-01-01

    The radioadaptive response is a phenomenon where exposure to a prior low dose of radiation reduces the level of damage induced by a subsequent high radiation dose. The molecular mechanism behind this is still not well understood. Learning more about the radioadaptive response is critical for long duration spaceflight since astronauts are exposed to low levels of cosmic radiation. The micronucleus assay was used to measure the level of damage caused by radiation. Although cells which were not washed with phosphate buffered saline (PBS) after a low priming dose of 5cGy did not show adaptation to the challenge dose, washing the cells with PBS and giving the cells fresh media after the low dose did allow radioadaptation to occur. This is consistent with the results of a previous publication by another research group. In the present study, genes involved in DNA damage signaling and the oxidative stress response were studied using RT PCR techniques in order to look at changes in expression level after the low dose with or without washing. Our preliminary results indicate that upregulation of oxidative stress response genes ANGPTL7, NCF2, TTN, and SRXN1 may be involved in the radioadaptive response. The low dose of radiation alone was found to activate the oxidative stress response genes GPR156 and MTL5, whereas, washing the cells alone caused relatively robust upregulation of the oxidative stress response genes DUSP1 and PTGS2. Washing after the priming dose showed some changes in the expression level of several DNA damage signaling genes. In addition, we studied whether washing the cells after the priming dose has an effect on the level of nitric oxide in both the media and cells, since nitric oxide levels are known to increase in the media of the cells after a high dose of radiation only if the cells were already exposed to a low priming dose. Based on this preliminary study, we propose that washing the cells after priming exposure actually eliminates some factor

  3. Imidacloprid does not induce Cyp genes involved in insecticide resistance of a mutant Drosophila melanogaster line.

    Science.gov (United States)

    Kalajdzic, Predrag; Markaki, Maria; Oehler, Stefan; Savakis, Charalambos

    2013-10-01

    Certain xenobiotics have the capacity to induce the expression of genes involved in various biological phenomena, including insecticide resistance. The induction potential of different chemicals, among them different insecticides, has been documented for a number of insect species. In this study, we have analyzed the induction potential of Imidacloprid, a widely used member of the neonicotinoid insecticide family. Genes Cyp6g1 and Cyp6a2, known to be involved in the resistance of mutant Drosophila melanogaster line MiT[W⁻]3R2 to Imidacloprid and DDT were included in the analyzed sample. We find that Imidacloprid does not induce expression of the analyzed genes. Copyright © 2013 Elsevier Ltd. All rights reserved.

  4. Estratégias de uso de água salobra na produção de alface em hidroponia NFT Strategies for use of brackish water in NFT hydroponic lettuce production

    Directory of Open Access Journals (Sweden)

    Márcio S. Alves

    2011-05-01

    Full Text Available Plantas de alface crespa 'Verônica' foram cultivadas em condições hidropônicas, objetivando avaliar três diferentes estratégias de emprego de águas salobras: 1 águas salobras para reposição das perdas por evapotranspiração (ETc e água doce para o preparo da solução nutritiva (SN; 2 águas salobras para o preparo da SN e água doce para reposição da ETc; 3 águas salobras para o preparo da SN e reposição da ETc. Os níveis de salinidade da água foram obtidos pela adição de NaCl à água doce (0,27 dS m-1: 1,45; 2,51; 3,6; 5,41 e 7,5 dS m-1. O experimento foi conduzido em quatro blocos aleatorizados, com quatro repetições por tratamento. Uma estrutura de pesquisa foi construída com 72 parcelas que simulam a técnica do fluxo laminar de nutrientes (NFT. O uso de água salobra apenas para repor a ETc não produziu efeito sobre a produção da alface. Por outro lado, o uso de águas salobras para o preparo da SN e água doce para reposição da ETc, reduziu o rendimento da alface (massa de matéria fresca da parte aérea em 4,99% por (dS m-1. O rendimento foi reduzido em 7% por dS m-1 quando águas salobras foram usadas tanto para o preparo da SN quanto para reposição da ETc. Apesar da redução linear da produção da alface com o aumento da salinidade da água, sintomas depreciativos para a qualidade da alface hidropônica não foram registrados.Plants of crisphead lettuce cv. 'Verônica' were grown under hydroponic conditions aiming to evaluate three different strategies of brackish waters utilization: 1 brackish waters to replace the evapotranspiration loss (ETc and fresh water to prepare nutrient solution (NS; 2 brackish waters to prepare NS and fresh water to replace the ETc; 3 brackish waters to prepare NS and replace ETc. The levels of water salinity were obtained by addition of NaCl to fresh water (0.27 dS m-1: 1.45; 2.51; 3.6; 5.41 and 7.5 dS m-1. The experiment was conducted in randomized blocks with four

  5. An ethylene-responsive enhancer element is involved in the senescence-related expression of the carnation glutathione-S-transferase (GST1) gene.

    Science.gov (United States)

    Itzhaki, H; Maxson, J M; Woodson, W R

    1994-09-13

    The increased production of ethylene during carnation petal senescence regulates the transcription of the GST1 gene encoding a subunit of glutathione-S-transferase. We have investigated the molecular basis for this ethylene-responsive transcription by examining the cis elements and trans-acting factors involved in the expression of the GST1 gene. Transient expression assays following delivery of GST1 5' flanking DNA fused to a beta-glucuronidase receptor gene were used to functionally define sequences responsible for ethylene-responsive expression. Deletion analysis of the 5' flanking sequences of GST1 identified a single positive regulatory element of 197 bp between -667 and -470 necessary for ethylene-responsive expression. The sequences within this ethylene-responsive region were further localized to 126 bp between -596 and -470. The ethylene-responsive element (ERE) within this region conferred ethylene-regulated expression upon a minimal cauliflower mosaic virus-35S TATA-box promoter in an orientation-independent manner. Gel electrophoresis mobility-shift assays and DNase I footprinting were used to identify proteins that bind to sequences within the ERE. Nuclear proteins from carnation petals were shown to specifically interact with the 126-bp ERE and the presence and binding of these proteins were independent of ethylene or petal senescence. DNase I footprinting defined DNA sequences between -510 and -488 within the ERE specifically protected by bound protein. An 8-bp sequence (ATTTCAAA) within the protected region shares significant homology with promoter sequences required for ethylene responsiveness from the tomato fruit-ripening E4 gene.

  6. Estudo comparativo da qualidade da água da chuva coletada em telhado com telhas de concreto e em telhado verde para usos não potáveis

    Directory of Open Access Journals (Sweden)

    Celimar Azambuja Teixeira

    Full Text Available Resumo Este artigo apresenta uma comparação da qualidade da água da chuva coletada em telhado inclinado com telhas de concreto (residência 1 e cobertura plana e vegetada (residência 2, indicada como telhado verde. Os objetivos desta investigação foram: (1 analisar a qualidade da água da chuva captada nas duas coberturas, com base nos parâmetros da NBR 15527 (ABNT, 2007 e (2 fazer acompanhamento da qualidade da água armazenada para verificar eficiência e comportamento dos dois telhados. Este estudo foi desenvolvido em Curitiba, PR, onde é obrigatório o aproveitamento da água da chuva em novas construções desde o ano de 2007. A água da chuva captada no telhado com telhas de concreto apresentou menores valores para turbidez e demanda química de oxigênio na maioria dos resultados (oito e sete amostras, respectivamente. O telhado verde contribuiu para a diminuição da acidez natural da água da chuva. Não foram atendidos os parâmetros microbiológicos estabelecidos na NBR 15527 (ABNT, 2007 em nenhuma das amostras coletadas. Apesar de a qualidade da água da chuva não atender todos os parâmetros indicados na NBR 15527 (ABNT, 2007, essa água vem sendo aproveitada nas residências para limpeza de calçadas, irrigação de jardim e descarga em bacias sanitárias, sem indicação de inconvenientes pelos moradores.

  7. Identification of Phytophthora sojae genes involved in asexual ...

    Indian Academy of Sciences (India)

    ual sporulation or germination. But molecular details about asexual spore development in P. sojae are limited (Tyler et al. 2006). In the present study, to understand the molecular basis of asexual spore development in P. sojae, we investigated gene expression changes involved in asexual sporulation after ul- traviolet (UV) ...

  8. Emergência e crescimento inicial de plântulas de albízia submetidas à irrigação com água salina

    Directory of Open Access Journals (Sweden)

    Mayky F. P. de Lima

    2015-02-01

    Full Text Available Objetivou-se, com este trabalho, avaliar o efeito de diferentes concentrações de sais na água de irrigação sob a emergência e o crescimento inicial de plântulas de albízia (Albizia lebbeck (L. Benth.. O experimento foi desenvolvido em casa de vegetação do departamento de Ciências Vegetais da Universidade Federal Rural do Semiárido, Mossoró, RN, usando-se o delineamento inteiramente casualizado, com seis tratamentos e quatro repetições, com 24 sementes em cada parcela. Os tratamentos foram constituídos das concentrações salinas 0,68 (água de abastecimento; 1,5; 3,0; 4,5; 6,0 e 7,5 dS m-1, obtidas através da adição de NaCl em água. As variáveis avaliadas foram: porcentagem de emergência de plântulas, índice de velocidade de emergência, comprimento da parte aérea, comprimento de raiz, número de folíolos, condutividade elétrica acumulada do substrato, índice de conteúdo de clorofila, massa seca da raiz, do caule, das folhas e total. O aumento da salinidade na água de irrigação interfere negativamente na emergência e no crescimento inicial de plântulas de albízia, sendo recomendado água com concentração salina até 1,5 dS m-1, caracterizando esta espécie como glicófita, sensível à salinidade.

  9. Genes and Gut Bacteria Involved in Luminal Butyrate Reduction Caused by Diet and Loperamide.

    Science.gov (United States)

    Hwang, Nakwon; Eom, Taekil; Gupta, Sachin K; Jeong, Seong-Yeop; Jeong, Do-Youn; Kim, Yong Sung; Lee, Ji-Hoon; Sadowsky, Michael J; Unno, Tatsuya

    2017-11-28

    Unbalanced dietary habits and gut dysmotility are causative factors in metabolic and functional gut disorders, including obesity, diabetes, and constipation. Reduction in luminal butyrate synthesis is known to be associated with gut dysbioses, and studies have suggested that restoring butyrate formation in the colon may improve gut health. In contrast, shifts in different types of gut microbiota may inhibit luminal butyrate synthesis, requiring different treatments to restore colonic bacterial butyrate synthesis. We investigated the influence of high-fat diets (HFD) and low-fiber diets (LFD), and loperamide (LPM) administration, on key bacteria and genes involved in reduction of butyrate synthesis in mice. MiSeq-based microbiota analysis and HiSeq-based differential gene analysis indicated that different types of bacteria and genes were involved in butyrate metabolism in each treatment. Dietary modulation depleted butyrate kinase and phosphate butyryl transferase by decreasing members of the Bacteroidales and Parabacteroides . The HFD also depleted genes involved in succinate synthesis by decreasing Lactobacillus . The LFD and LPM treatments depleted genes involved in crotonoyl-CoA synthesis by decreasing Roseburia and Oscilllibacter . Taken together, our results suggest that different types of bacteria and genes were involved in gut dysbiosis, and that selected treatments may be needed depending on the cause of gut dysfunction.

  10. Gene expression profiling following maternal deprivation: Involvement of the brain renin-angiotensin system

    Directory of Open Access Journals (Sweden)

    Claudia Liebl

    2009-05-01

    Full Text Available The postnatal development of the mouse is characterized by a stress hyporesponsive period (SHRP, where basal corticosterone levels are low and responsiveness to mild stressors is reduced. Maternal separation is able to disrupt the SHRP and is widely used to model early trauma. In this study we aimed at identifying of brain systems involved in acute and possible long-term effects of maternal separation. We conducted a microarray-based gene expression analysis in the hypothalamic paraventricular nucleus after maternal separation, which revealed 52 differentially regulated genes compared to undisturbed controls, among them are 37 up-regulated and 15 down-regulated genes. One of the prominently up-regulated genes, angiotensinogen, was validated using in-situ hybridization. Angiotensinogen is the precursor of angiotensin II, the main effector of the brain renin-angiotensin system (RAS, which is known to be involved in stress system modulation in adult animals. Using the selective angiotensin type I receptor (AT(1 antagonist candesartan we found strong effects on CRH and GR mRNA expression in the brain a nd ACTH release following maternal separation. AT(1 receptor blockade appears to enhance central effects of maternal separation in the neonate, suggesting a suppressing function of brain RAS during the SHRP. Taken together, our results illustrate the molecular adaptations that occur in the paraventricular nucleus following maternal separation and contribute to identifying signaling cascades that control stress system activity in the neonate.

  11. Identification of Plagl1/Zac1 binding sites and target genes establishes its role in the regulation of extracellular matrix genes and the imprinted gene network.

    Science.gov (United States)

    Varrault, Annie; Dantec, Christelle; Le Digarcher, Anne; Chotard, Laëtitia; Bilanges, Benoit; Parrinello, Hugues; Dubois, Emeric; Rialle, Stéphanie; Severac, Dany; Bouschet, Tristan; Journot, Laurent

    2017-10-13

    PLAGL1/ZAC1 undergoes parental genomic imprinting, is paternally expressed, and is a member of the imprinted gene network (IGN). It encodes a zinc finger transcription factor with anti-proliferative activity and is a candidate tumor suppressor gene on 6q24 whose expression is frequently lost in various neoplasms. Conversely, gain of PLAGL1 function is responsible for transient neonatal diabetes mellitus, a rare genetic disease that results from defective pancreas development. In the present work, we showed that Plagl1 up-regulation was not associated with DNA damage-induced cell cycle arrest. It was rather associated with physiological cell cycle exit that occurred with contact inhibition, growth factor withdrawal, or cell differentiation. To gain insights into Plagl1 mechanism of action, we identified Plagl1 target genes by combining chromatin immunoprecipitation and genome-wide transcriptomics in transfected cell lines. Plagl1-elicited gene regulation correlated with multiple binding to the proximal promoter region through a GC-rich motif. Plagl1 target genes included numerous genes involved in signaling, cell adhesion, and extracellular matrix composition, including collagens. Plagl1 targets also included 22% of the 409 genes that make up the IGN. Altogether, this work identified Plagl1 as a transcription factor that coordinated the regulation of a subset of IGN genes and controlled extracellular matrix composition. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  12. Trocas gasosas e conteúdo de carboidratos e compostos nitrogenados em pinhão-manso irrigado com águas residuária e salina

    Directory of Open Access Journals (Sweden)

    Antonio Evami Cavalcante Sousa

    2012-10-01

    Full Text Available O objetivo deste trabalho foi avaliar alterações nas trocas gasosas e nos conteúdos de carboidratos e compostos nitrogenados, em pinhão-manso (Jatropha curcas irrigado com águas residuária e salina. Empregou-se o delineamento em blocos ao acaso, com quatro tratamentos e quatro repetições: irrigação plena com água de abastecimento a 0,6 dS m-1 (controle; irrigação plena com água salinizada a 2,4 dS m-1 (SAL; e irrigação com água residuária de esgoto, plena (R100 e a 50% da capacidade de campo (R50. O tratamento R50 reduziu fortemente as trocas gasosas e a área foliar, comparado ao controle, seguido pelos tratamentos SAL e R100. A redução na fotossíntese diminuiu o nível de sacarose nas folhas, nos quatro tratamentos. Os teores de açúcares solúveis aumentaram nos tratamentos R50, SAL e R100, enquanto o conteúdo de amido permaneceu praticamente inalterado. Os conteúdos de prolina e glicina betaína aumentaram nos três tratamentos, mas a última foi mais importante, em termos quantitativos, como protetor celular e osmótico. A irrigação plena com água residuária induz efeitos similares aos causados pela irrigação com água salina. A irrigação limitada com água residuária causa estresse agudo às plantas, provavelmente pela combinação de deficiência hídrica e acúmulo de solutos no solo.

  13. Transcriptome profiling of the Plutella xylostella (Lepidoptera: Plutellidae) ovary reveals genes involved in oogenesis.

    Science.gov (United States)

    Peng, Lu; Wang, Lei; Yang, Yi-Fan; Zou, Ming-Min; He, Wei-Yi; Wang, Yue; Wang, Qing; Vasseur, Liette; You, Min-Sheng

    2017-12-30

    As a specialized organ, the insect ovary performs valuable functions by ensuring fecundity and population survival. Oogenesis is the complex physiological process resulting in the production of mature eggs, which are involved in epigenetic programming, germ cell behavior, cell cycle regulation, etc. Identification of the genes involved in ovary development and oogenesis is critical to better understand the reproductive biology and screening for the potential molecular targets in Plutella xylostella, a worldwide destructive pest of economically major crops. Based on transcriptome sequencing, a total of 7.88Gb clean nucleotides was obtained, with 19,934 genes and 1861 new transcripts being identified. Expression profiling indicated that 61.7% of the genes were expressed (FPKM≥1) in the P. xylostella ovary. GO annotation showed that the pathways of multicellular organism reproduction and multicellular organism reproduction process, as well as gamete generation and chorion were significantly enriched. Processes that were most likely relevant to reproduction included the spliceosome, ubiquitin mediated proteolysis, endocytosis, PI3K-Akt signaling pathway, insulin signaling pathway, cAMP signaling pathway, and focal adhesion were identified in the top 20 'highly represented' KEGG pathways. Functional genes involved in oogenesis were further analyzed and validated by qRT-PCR to show their potential predominant roles in P. xylostella reproduction. Our newly developed P. xylostella ovary transcriptome provides an overview of the gene expression profiling in this specialized tissue and the functional gene network closely related to the ovary development and oogenesis. This is the first genome-wide transcriptome dataset of P. xylostella ovary that includes a subset of functionally activated genes. This global approach will be the basis for further studies on molecular mechanisms of P. xylostella reproduction aimed at screening potential molecular targets for integrated pest

  14. Crescimento, consumo hídrico e composição mineral de alface cultivada em hidroponia com águas salinas

    Directory of Open Access Journals (Sweden)

    Dalva Paulus

    2012-02-01

    Full Text Available Objetivou-se, com este trabalho, avaliar o crescimento, o consumo hídrico e a extração de nutrientes, por alface (Lactuca sativa L. em sistema hidropônico NFT (fluxo laminar de nutrientes, com a utilização de águas salinas no preparo da solução nutritiva e reposição da lâmina diária evapotranspirada. Foram conduzidos dois experimentos, em ambiente protegido. O delineamento experimental foi em blocos ao acaso, sendo estudados os efeitos de cinco níveis de salinidade da água, utilizando-se NaCl. O aumento da salinidade da água reduziu, de forma linear, o crescimento e o consumo hídrico da alface. A salinidade reduziu a concentração de macronutrientes na parte aérea, mas não se observaram sintomas de deficiência nutricional. O rendimento (massa de matéria fresca da parte aérea foi reduzido entre 6 e 6,5% por dS m-1, no primeiro e segundo cultivo, respectivamente. Os resultados obtidos em sistema de cultivo NFT podem indicar a possibilidade do uso da água salina como alternativa para produção de hortaliças, para produtores que têm disponibilidade restrita de água doce, porém, com redução de produtividade.

  15. Água viva: orienta(lização modernista

    Directory of Open Access Journals (Sweden)

    Larissa Costa da Mata

    2007-01-01

    Full Text Available The Brazilian modernist canon is usually associated to the writers who took part in the Week of Modern Art in 1922, whose works are analyzed considering structure as the main feature. This paper aims to analyze Água viva (1973, written by Clarice Lispector, a dissident modernist poet, by means of Taoism, philosophy that proposes the Tao, a heterogeneous unity, reminding a cyclical culture producer of strain. In Taoism, as in Chinese calligraphy, the shape is undone, giving back strength to the shapeless matter; features that enable a change in the Western way of dealing with art; emphasizing strength, instead of shape. This “Eastern heritage” may be found in the Brazilian artist Maria Martins (1894-1973, who wrote Ásia Maior: o planeta China (1958 and as in the Mexican writer Octavio Paz (1914-1998, author of Conjunciones y disyunciones (1969; works which will be essential to consider the East’s presence in Água viva. The increasing interest in the East influences on the cultural studies and it becomes a tool to the post- structuralist theories deal with post-modern hybridism. In this paper, the East will be useful to analyze Água viva, producing new connections with Brazilian modernism allowing different means of dissemination of this work and of this literary movement.

  16. Identification of transcription coactivator OCA-B-dependent genes involved in antigen-dependent B cell differentiation by cDNA array analyses.

    Science.gov (United States)

    Kim, Unkyu; Siegel, Rachael; Ren, Xiaodi; Gunther, Cary S; Gaasterland, Terry; Roeder, Robert G

    2003-07-22

    The tissue-specific transcriptional coactivator OCA-B is required for antigen-dependent B cell differentiation events, including germinal center formation. However, the identity of OCA-B target genes involved in this process is unknown. This study has used large-scale cDNA arrays to monitor changes in gene expression patterns that accompany mature B cell differentiation. B cell receptor ligation alone induces many genes involved in B cell expansion, whereas B cell receptor and helper T cell costimulation induce genes associated with B cell effector function. OCA-B expression is induced by both B cell receptor ligation alone and helper T cell costimulation, suggesting that OCA-B is involved in B cell expansion as well as B cell function. Accordingly, several genes involved in cell proliferation and signaling, such as Lck, Kcnn4, Cdc37, cyclin D3, B4galt1, and Ms4a11, have been identified as OCA-B-dependent genes. Further studies on the roles played by these genes in B cells will contribute to an understanding of B cell differentiation.

  17. Involvement of interleukin-1 in lead nitrate-induced hypercholesterolemia in mice.

    Science.gov (United States)

    Kojima, Misaki; Ashino, Takashi; Yoshida, Takemi; Iwakura, Yoichiro; Degawa, Masakuni

    2012-01-01

    Hepatic 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and cholesterol 7α-hydroxylase (Cyp7a1) are rate-limiting enzymes for cholesterol biosynthesis and catabolism, respectively. Involvement of inflammatory cytokines, particularly interleukin-1 (IL-1), in alterations of HMGR and Cyp7a1 gene expression during development of lead nitrate (LN)-induced hypercholesterolemia was examined in IL-1α/β-knockout (IL-1-KO) and wild-type (WT) mice. Lead nitrate treatment of WT mice led to not only a marked downregulation of the Cyp7a1 gene at 6-12 h, but also a significant upregulation of the HMGR gene at 12 h. However, such changes were not observed at significant levels in IL-1-KO mice, although a slight, transient downregulation of the Cyp7a1 gene and a minimal upregulation of the HMGR gene occurred at 6 h and 24 h, respectively. Consequently, LN treatment led to development of hypercholesterolemia at 24 h in WT mice, but not in IL-1-KO mice. Furthermore, in WT mice, significant LN-mediated increases were observed at 3-6 h in hepatic IL-1 levels, which can modulate gene expression of Cyp7a1 and HMGR. These findings indicate that, in mice, LN-mediated increases in hepatic IL-1 levels contribute, at least in part, to altered expressions of Cyp7a1 and HMGR genes, and eventually to hypercholesterolemia development.

  18. Analysis of gene expression of myo1c and inpp5k genes involved in endometrial adenocarcinoma

    International Nuclear Information System (INIS)

    Koul, A.M.; Nadeem, A.; Baryalai, P.

    2012-01-01

    Abstract: Inpp5k gene encodes a protein which plays a very vital role in a number of metabolic pathways. It is very significant in the glucose metabolism where it regulates the signalling of the insulin pathway. But the full molecular details of the pathways regulated by Inpp5k encoded protein are not known. It is speculated that Inpp5k gene expression is altered in case of endometrial adenocarcinoma. Myolc gene encodes for a protein called Myosin-lc which acts an actin-based molecular motor in the cells. II has been studied that this gene down-regulates during endometrial adenocarcinoma and colorectal cancers. In this study the expression analysis of these two was carried out using multiplex PCR. An endogenous control was used for this PCR. ACTS gene served as the endogenous control because of it being a house keeping gene. It thus shows a universal expression in all cells. Thus in this study the gene expression of Inpp5k and Myulc genes was comparatively analysed with ACTS gene. The results that came out of this study showed an over-expression of Inpp5k gene and down-regulation of myolc gene with respect to ACTS gene in cancer cell lines as was indicated by the previous studies with these genes. Expression of both genes i.e. Inpp5k and Myolc was statistically compared between normal and cancerous cell lines and was found statistically significant at a value of P< O.O I in most of the cases. (author)

  19. GESTÃO DA ÁGUA EM USINA DE CONCRETO: ANALISE DO RISCO DAS ATIVIDADES E MONITORAMENTO DA QUALIDADE DA ÁGUA RESIDUÁRIA PARA FINS DE REUSO (DOI.:10.5216/reec.V10i1.32759

    Directory of Open Access Journals (Sweden)

    Heber Martins de Paula

    2015-07-01

    Full Text Available RESUMO: As usinas de concreto vêm enfrentando o desafio de aumentar a produção, reduzindo o volume de resíduos gerados. Suas principais atividades demandam de volumes consideráveis de água que, ao mesmo tempo, não devem ser descartadas no solo ou redes coletoras, pois podem gerar riscos ao meio ambiente. Assim, este artigo tem como objetivo avaliar a frequência e o risco das atividades da usina quanto ao uso da água. Além disso, monitorar a qualidade da água do sistema de tratamento quanto ao pH e a turbidez. Para tanto, foi aplicado o método de Avaliação de Risco da Atividade aliado a observações realizadas in loco para melhor representar as ações de um dia convencional de produção. O levantamento foi realizado por seis dias aleatórios por três semanas consecutivas. Para avaliação da qualidade da água residuária foram monitorados o pH e a turbidez, sempre no último dia de produção da usina, representando a pior situação. Os resultados mostraram que a lavagem dos caminhões é a atividade que apresenta maior risco ao meio ambiente, pois acontece com maior frequência dentro do espaço de produção. O sistema atual não consegue remover a turbidez para destinação da água ao reuso, mantendo o pH acima de 12. ABSTRACT: The concrete plants are facing the challenge of increasing production, reducing the volume of waste generated. Its main activities require considerable volumes of water at the same time, should not be dismissed on the ground or collecting networks, they could generate risks to the environment. Thus, this article aims to evaluate the frequency and risk of the activities of the concrete plant on the use of water. In addition, monitor the quality of water treatment system for pH and turbidity. To this end, we applied the method of the Activity Risk Assessment together with in situ observations to better represent the actions of a conventional production day. The survey was conducted by six random days

  20. Desenvolvimento vegetativo do pepino enxertado irrigado com água salina Vegetative development on grafted cucumber plants irrigated with saline water

    Directory of Open Access Journals (Sweden)

    Marcos Vinícius Folegatti

    2000-09-01

    Full Text Available A salinização dos solos em ambiente protegido devido ao excesso de fertilizantes e falta de lixiviação tem resultado na redução da produtividade das culturas. Este trabalho teve o objetivo de avaliar os efeitos da irrigação com água salina no desenvolvimento vegetativo do pepino enxertado cultivado em ambiente protegido. Foram utilizadas águas de diferentes salinidades (S1=1,58; S2=3,08 e S3=5,13 dS m-1, lâminas de água de irrigação (L0=1,00 x ETc e L1=1,25 x ETc e freqüências de aplicação da lâmina L1 (F1=em todas as irrigações e F2=quando a lâmina de água de irrigação acumulada em L0 atingia 100 mm. Os resultados demonstraram que a altura das plantas, área foliar unitária e índice de área foliar foram afetados linearmente pela salinidade da água, não apresentando diferença para as diferentes lâminas e frequências de aplicação de L1.Greenhouse soil salinization by excessive fertilization and lack of leaching has been a common cause of cucumber yield reduction in Brazil. The aim of this work was to evaluate the effects of irrigation with saline water on the vegetative development of grafted cucumber plants in a greenhouse. Three water salinities (S1=1.58; S2=3.08 e S3=5.13 dS m-1, two irrigation water depths (L0=1.00 x ETc e L1=1.25 x ETc and two application frequencies of L1 (F1=in all irrigations and F2=when the irrigation water depth of L0 reached 100 mm were used. Irrigation water depths and frequencies of L1 were grouped and, therefore, the experimental design was in a factorial scheme 3x3, with randomized blocks. Results showed that plant height, unit leaf area and leaf area index were linearly affected by water salinity. No differences were observed for the various irrigation water depths and frequencies of L1 application.

  1. Resposta de plantas de melancieira irrigadas com água salina ao tratamento pré-germinativo da semente com H2O2

    Directory of Open Access Journals (Sweden)

    Auderlan de Macena Pereira

    2013-10-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE O manejo da água é um dos fatores mais importantes para uma efetiva absorção de nutrientes, uma vez que esta pode apresentar-se com elevadas concentrações de sais, o que pode afetar o processo de absorção, bem como causar estresse oxidativo nos vegetais, devido as espécies reativas de oxigênios (EROs, principalmente H2O2. As plantas evoluíram um complexo sistema antioxidante para conter os efeitos deletérios das EROs, é possível que a pré-exposição das plantas ao H2O2 possibilite uma adaptação das mesmas. Estudando-se uma alternativa para amenizar os efeitos deletérios das EROs,objetivou-se, neste trabalho, avaliar a resposta de plantas de melancieira, irrigadas com água salina, ao tratamento pré-germinativo da semente com H2O2. O experimento foi conduzido no Centro de Ciências e Tecnologia Agroalimentar-CCTA, da UFCG-Pombal – PB. O delineamento experimental utilizado foi de blocos casualizados em parcelas subdivididas 2x5, com 4 repetições, com a parcela constando de dois níveis de salinidade da água de irrigação (S1 = água de abastecimento local, 0,32 dSm-1; e S2 =  água salina, 2,0 dS m-1 e a subparcela de sementes de melancieira tratadas com solução de H2O2 em cinco concentrações (0, 5, 10, 15 e 20 µmol L-1. As aplicações foram feitas via água de irrigação, junto à solução nutritiva a 100%, utilizada como fonte de nutrientes. O experimento foi conduzido até o início da floração (45 DAS, quando foram avaliadas as seguintes características de crescimento: Massa Fresca da Parte Aérea, Massa Seca da Parte Aérea, Número de Folhas por Planta e Comprimento da Haste Principal. Avalio-use também as características fisiológicas: Taxa de Fotossíntese, Transpiração, Condutância Estomática e Concentração de CO2 intracelular. Os dados foram submetidos à análise de variância ao nível de 5 % de probabilidade. Os níveis de salinidades da água

  2. Avaliação energética de um sistema integrado de abastecimento de água

    Directory of Open Access Journals (Sweden)

    Ana Luiza Rezende Guanais

    2017-07-01

    Full Text Available RESUMO Os encargos energéticos decorrentes do Sistema Integrado de Abastecimento de Água de Feira de Santana (SIAA-FSA foram analisados utilizando o método de avaliação do ciclo de vida. Foram identificadas, na cadeia de abastecimento de água, as etapas do sistema com maiores encargos energéticos, e propostos cenários de melhoria. Cadeias de produção dos insumos químicos, transporte dos insumos, energia elétrica e substituição dos tubos na rede para a manutenção do SIAA-FSA foram consideradas. A demanda energética acumulada do SIAA-FSA foi de 3,51 kWh.m-3 de água consumida. As etapas de captação e distribuição de água apresentaram os maiores consumos energéticos, e a eletricidade do bombeamento representou 86% da demanda energética acumulada. Os cenários propostos para o SIAA-FSA apresentaram melhorias significativas em relação ao cenário atual, com usos mais racionais de insumos químicos, eletricidade e água.

  3. The involvement of wheat F-box protein gene TaFBA1 in the oxidative stress tolerance of plants.

    Directory of Open Access Journals (Sweden)

    Shu-Mei Zhou

    Full Text Available As one of the largest gene families, F-box domain proteins have been found to play important roles in abiotic stress responses via the ubiquitin pathway. TaFBA1 encodes a homologous F-box protein contained in E3 ubiquitin ligases. In our previous study, we found that the overexpression of TaFBA1 enhanced drought tolerance in transgenic plants. To investigate the mechanisms involved, in this study, we investigated the tolerance of the transgenic plants to oxidative stress. Methyl viologen was used to induce oxidative stress conditions. Real-time PCR and western blot analysis revealed that TaFBA1 expression was up-regulated by oxidative stress treatments. Under oxidative stress conditions, the transgenic tobacco plants showed a higher germination rate, higher root length and less growth inhibition than wild type (WT. The enhanced oxidative stress tolerance of the transgenic plants was also indicated by lower reactive oxygen species (ROS accumulation, malondialdehyde (MDA content and cell membrane damage under oxidative stress compared with WT. Higher activities of antioxidant enzymes, including superoxide dismutase (SOD, catalase (CAT, ascorbate peroxidase (APX and peroxidase (POD, were observed in the transgenic plants than those in WT, which may be related to the upregulated expression of some antioxidant genes via the overexpression of TaFBA1. In others, some stress responsive elements were found in the promoter region of TaFBA1, and TaFBA1 was located in the nucleus, cytoplasm and plasma membrane. These results suggest that TaFBA1 plays an important role in the oxidative stress tolerance of plants. This is important for understanding the functions of F-box proteins in plants' tolerance to multiple stress conditions.

  4. O AQÜÍFERO GUARANI E SEUS PARÂMETROS JURÍDICOS: PERSPECTIVAS E LÓGICAS DA ESCASSEZ DE ÁGUA DOCE

    Directory of Open Access Journals (Sweden)

    Christian Guy Caubet

    2012-08-01

    Full Text Available O Aqüífero Guarani não é um tanque de água subterrâneo. É um conjunto fraturado de eventuais oportunidades de obter água potável em diversos lugares. Quando usadas suas águas, cria-se espaço para receber águas superficiais eventualmente contaminadas. O balanço atual do uso das águas de superfície e subterrâneas, no mundo todo, evidencia que a mercantilização substitui rapidamente as antigas obrigações de políticas públicas de providenciar água de qualidade em quantidades suficientes para o conjunto da população. As normas nacionais e internacionais recém adotadas não são suficientes para reverter o quadro atual.

  5. Metemoglobinemia e nitrato nas águas Methemoglobin and nitrate levels in drinking water

    Directory of Open Access Journals (Sweden)

    Nilda Gallego Gándara de Fernícola

    1981-04-01

    Full Text Available Foram determinados os níveis de metemoglobina em 116 crianças (idade média de 15 meses do Estado de São Paulo (Brasil. Dois grupos foram formados: Grupo 1, por 92 crianças que consumiam água com teores de nitrato inferiores ao limite de 10 mg N/litro; e Grupo 2, por 24 crianças que bebiam água de poço com níveis de nitratos superiores ao permitido. A metemoglobinemia média do Grupo 1 (0,56% mostrou-se estatisticamente diferente e inferior (alfa = 0,05 à do Grupo 2 (0,76%.Methemoglobin levels were determined in the blood samples of 116 children (average age, 15 months living in the State of S. Paulo (Brazil. Blood from two groups was analyzed: Group 1 was formed by 92 children who drank water with nitrate levels below 10 mg N/liter; whereas Group 2 was formed by 24 children who drank well water with nitrate above the level permitted. The methemoglobin average for Group 1 (0.56% was statistically different and less that for Group 2 (0.76%.

  6. Resveratrol stimulates c-Fos gene transcription via activation of ERK1/2 involving multiple genetic elements.

    Science.gov (United States)

    Thiel, Gerald; Rössler, Oliver G

    2018-06-05

    The polyphenol resveratrol is found in many plant and fruits and is a constituent of our diet. Resveratrol has been proposed to have chemopreventive and anti-inflammatory activities. On the cellular level, resveratrol activates stimulus-regulated transcription factors. To identify resveratrol-responsive elements within a natural gene promoter, the molecular pathway leading to c-Fos gene expression by resveratrol was dissected. The c-Fos gene encodes a basic region leucine zipper transcription factor and is a prototype of an immediate-early gene that is regulated by a wide range of signaling molecules. We analyzed chromatin-integrated c-Fos promoter-luciferase reporter genes where transcription factor binding sites were destroyed by point mutations or deletion mutagenesis. The results show that mutation of the binding sites for serum response factor (SRF), activator protein-1 (AP-1) and cAMP response element binding protein (CREB) significantly reduced reporter gene transcription following stimulation of the cells with resveratrol. Inactivation of the binding sites for signal transducer and activator of transcription (STAT) or ternary complex factors did not influence resveratrol-regulated c-Fos promoter activity. Thus, the c-Fos promoter contains three resveratrol-responsive elements, the cAMP response element (CRE), and the binding sites for SRF and AP-1. Moreover, we show that the transcriptional activation potential of the c-Fos protein is increased in resveratrol-stimulated cells, indicating that the biological activity of c-Fos is elevated by resveratrol stimulation. Pharmacological and genetic experiments revealed that the protein kinase ERK1/2 is the signal transducer that connects resveratrol treatment with the c-Fos gene. Copyright © 2018 Elsevier B.V. All rights reserved.

  7. Carboxylesterase 1 genes

    DEFF Research Database (Denmark)

    Rasmussen, Henrik Berg; Madsen, Majbritt Busk

    2018-01-01

    The carboxylesterase 1 gene (CES1) encodes a hydrolase that metabolizes commonly used drugs. The CES1-related pseudogene, carboxylesterase 1 pseudogene 1 (CES1P1), has been implicated in gene exchange with CES1 and in the formation of hybrid genes including the carboxylesterase 1A2 gene (CES1A2...

  8. Reuso de água na lavagem de veículos.

    OpenAIRE

    Eduardo Bronzatti Morelli

    2005-01-01

    O presente trabalho apresenta, em primeiro lugar, um levantamento bibliográfico sobre a conceituação de “reuso de água” em todas as suas modalidades. A seguir, uma análise da situação atual desta prática na Região Metropolitana de São Paulo, apresentando estudos em andamento na Companhia de Saneamento Básico do Estado de São Paulo – Sabesp e os aspectos relativos à elaboração de legislação específica sobre o assunto no país. Dando ênfase à reutilização das águas para lavagem de veículos, apre...

  9. Guanine limitation results in CodY-dependent and -independent alteration of Staphylococcus aureus physiology and gene expression.

    Science.gov (United States)

    King, Alyssa N; Borkar, Samiksha; Samuels, David J; Batz, Zachary; Bulock, Logan; Sadykov, Marat R; Bayles, Kenneth W; Brinsmade, Shaun R

    2018-04-30

    In Staphylococcus aureus , the global transcriptional regulator CodY modulates the expression of hundreds of genes in response to the availability of GTP and the branched-chain amino acids isoleucine, leucine, and valine (ILV). CodY DNA-binding activity is high when GTP and ILV are abundant. When GTP and ILV are limited, CodY's affinity for DNA drops, altering expression of CodY regulated targets. In this work, we investigated the impact of guanine nucleotides on S. aureus physiology and CodY activity by constructing a guaA null mutant (Δ guaA ). De novo biosynthesis of guanine monophosphate is abolished due to the guaA mutation; thus, the mutant cells require exogenous guanosine for growth. We also found that CodY activity was reduced when we knocked out guaA , activating the Agr two-component system and increasing secreted protease activity. Notably, in a rich, complex medium, we detected an increase in alternative sigma factor B activity in the Δ guaA mutant, which results in a 5-fold increase in production of the antioxidant pigment staphyloxanthin. Under biologically relevant flow conditions, Δ guaA cells failed to form robust biofilms when limited for guanine or guanosine. RNA-seq analysis of S. aureus transcriptome during growth in guanosine-limited chemostats revealed substantial CodY-dependent and -independent alteration of gene expression profiles. Importantly, these changes increase production of proteases and δ-toxin, suggesting that S. aureus exhibits a more invasive lifestyle when limited for guanosine. Further, gene-products upregulated under GN limitation, including those necessary for lipoic acid biosynthesis and sugar transport, may prove to be useful drug targets for treating Gram-positive infections. Importance Staphylococcus aureus infections impose a serious economic burden on healthcare facilities and patients because of the emergence of strains resistant to last-line antibiotics. Understanding the physiological processes governing

  10. SLC6A1 gene involvement in susceptibility to attention-deficit/hyperactivity disorder: A case-control study and gene-environment interaction.

    Science.gov (United States)

    Yuan, Fang-Fen; Gu, Xue; Huang, Xin; Zhong, Yan; Wu, Jing

    2017-07-03

    Attention-deficit/hyperactivity disorder (ADHD) is an early onset childhood neurodevelopmental disorder with an estimated heritability of approximately 76%. We conducted a case-control study to explore the role of the SLC6A1 gene in ADHD. The genotypes of eight variants were determined using Sequenom MassARRAY technology. The participants in the study were 302 children with ADHD and 411 controls. ADHD symptoms were assessed using the Conners Parent Symptom Questionnaire. In our study, rs2944366 was consistently shown to be associated with the ADHD risk in the dominant model (odds ratio [OR]=0.554, 95% confidence interval [CI]=0.404-0.760), and nominally associated with Hyperactive index score (P=0.027). In addition, rs1170695 has been found to be associated with the ADHD risk in the addictive model (OR=1.457, 95%CI=1.173-1.809), while rs9990174 was associated with the Hyperactive index score (P=0.010). Intriguingly, gene-environmental interactions analysis consistently revealed the potential interactions of rs1170695 with blood lead (P mul =0.044) to modify the ADHD risk. Expression quantitative trait loci analysis suggested that these positive single nucleotide polymorphisms (SNPs) may mediate SLC6A1 gene expression. Therefore, our results suggest that selected SLC6A1 gene variants may have a significant effect on the ADHD risk. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. Effects of long-term football training on the expression profile of genes involved in muscle oxidative metabolism

    DEFF Research Database (Denmark)

    Alfieri, A; Martone, D; Randers, Morten Bredsgaard

    2015-01-01

    and a muscle biopsy from the vastus lateralis were collected at T0 (pre intervention) and at T1 (post intervention). Gene expression was measured by RTqPCR on RNA extracted from muscle biopsies. The expression levels of the genes principally involved in energy metabolism (PPARγ, adiponectin, AMPKα1/α2, TFAM...... to improve the expression of muscle molecular biomarkers that are correlated to oxidative metabolism in healthy males....... are directly or indirectly involved in the glucose and lipid oxidative metabolism. Multiple linear regression analysis revealed that fat percentage was independently associated with NAMPT, PPARγ and adiponectin expression. In conclusion, long-term recreational football training could be a useful tool...

  12. Systematic study of association of four GABAergic genes: glutamic acid decarboxylase 1 gene, glutamic acid decarboxylase 2 gene, GABA(B) receptor 1 gene and GABA(A) receptor subunit beta2 gene, with schizophrenia using a universal DNA microarray.

    Science.gov (United States)

    Zhao, Xu; Qin, Shengying; Shi, Yongyong; Zhang, Aiping; Zhang, Jing; Bian, Li; Wan, Chunling; Feng, Guoyin; Gu, Niufan; Zhang, Guangqi; He, Guang; He, Lin

    2007-07-01

    Several studies have suggested the dysfunction of the GABAergic system as a risk factor in the pathogenesis of schizophrenia. In the present study, case-control association analysis was conducted in four GABAergic genes: two glutamic acid decarboxylase genes (GAD1 and GAD2), a GABA(A) receptor subunit beta2 gene (GABRB2) and a GABA(B) receptor 1 gene (GABBR1). Using a universal DNA microarray procedure we genotyped a total of 20 SNPs on the above four genes in a study involving 292 patients and 286 controls of Chinese descent. Statistically significant differences were observed in the allelic frequencies of the rs187269C/T polymorphism in the GABRB2 gene (P=0.0450, chi(2)=12.40, OR=1.65) and the -292A/C polymorphism in the GAD1 gene (P=0.0450, chi(2)=14.64 OR=1.77). In addition, using an electrophoretic mobility shift assay (EMSA), we discovered differences in the U251 nuclear protein binding to oligonucleotides representing the -292 SNP on the GAD1 gene, which suggests that the -292C allele has reduced transcription factor binding efficiency compared with the 292A allele. Using the multifactor-dimensionality reduction method (MDR), we found that the interactions among the rs187269C/T polymorphism in the GABRB2 gene, the -243A/G polymorphism in the GAD2 gene and the 27379C/T and 661C/T polymorphisms in the GAD1 gene revealed a significant association with schizophrenia (Pschizophrenia in the Chinese population.

  13. Construction Of An Optimized Lentiviral Vector Containing Pdx-1 Gene For Transduction Of Stem Cells Towards Gene Therapy Diabetes Type 1

    Directory of Open Access Journals (Sweden)

    S Rahmati

    2013-02-01

    Full Text Available Abstract Background & aim: Nowadays, most of gene therapy protocols are performed by lentiviral vectors. One of the most important factors which is involved in pancreas development and transcription of insulin gene is pancreatic & duodenal homeobox 1 (PDX-1 transcription factor. The goal of this study was to optimize a lentiviral construct, containing pdx-1 gene, to transfect stem cells towards gene therapy of type-1 diabetes. Methods: In this experimental study, first, the pdx-1 gene was multiplied by PCR from pcDNA3.1-pdx-1 and cloned into pTG19-T vector. Then, pdx-1 was subcloned on upstream of IRES-EGFP gene into IRES2-EGFP vector. At the next step, the cloned parts of IRES-EGFP and pdx-1 were isolated and cloned into the lentiviral expression vector pSINTREM in upstream of TRE-CMV gene. After sequencing, final construct was transfected into HEK 293 cells and gene expression of pdx-1 was evaluated using flow cytometry analysis and reverse fluorescent microscopy. Results: Flow cytometry results and inverted fluorescent microscopy observing showed that pdx-1 and GFP genes are expressed in cells transfected with final recombinant construct. Conclusion: Regarding the design of this construct, to ensure long time expression with higher in vivo and in vitro expression efficiency for stem cells and also use of Tet on induced optimized system, it seems that the current construct can be among the best ones to transfect stem cells. Key words: Gene therapy, Diabetes, Stem cells

  14. QUALIDADE DA ÁGUA ARMAZENADA EM RESERVATÓRIOS DOMICILIARES: PARÂMETROS FÍSICO-QUÍMICOS E MICROBIOLÓGICOS

    Directory of Open Access Journals (Sweden)

    Juliana Alvares Duarte Bonini CAMPOS

    2009-07-01

    Full Text Available

    Considerando-se que a água é um nutriente fundamental para manutenção da vida e reconhecendo-se a interferência dos diversos processos utilizados no tratamento da água na qualidade final do produto o presente estudo foi realizado visando verificar os efeitos dos reservatórios domiciliares nas características físico-químicas e microbiológicas da água utilizada para consumo humano na cidade de Araraquara (SP. Os resultados encontrados demonstraram discrepância nos teores de ferro e turbidez para amostras coletadas nos reservatórios domiciliares, teores de cloro abaixo do preconizado em 27% das amostras e contaminação por coliformes totais em 18,9% delas, monstrando que o reservatório domiciliar é fator deteriorador da água nele armazenada. PALAVRAS-CHAVE: Reservatórios domiciliares; água; análise microbiológica; cloro.

  15. The adaptor SASH1 acts through NOTCH1 and its inhibitor DLK1 in a 3D model of lumenogenesis involving CEACAM1.

    Science.gov (United States)

    Stubblefield, Kandis; Chean, Jennifer; Nguyen, Tung; Chen, Charng-Jui; Shively, John E

    2017-10-15

    CEACAM1 transfection into breast cancer cells restores lumen formation in a 3D culture model. Among the top up-regulated genes that were associated with restoration of lumen formation, the adaptor protein SASH1 was identified. Furthermore, SASH1 was shown to be critical for lumen formation by RNAi inhibition. Upon analyzing the gene array from CEACAM1/MCF7 cells treated with SASH1 RNAi, DLK1, an inhibitor of NOTCH1 signaling, was found to be down-regulated to the same extent as SASH1. Subsequent treatment of CEACAM1/MCF7 cells with RNAi to DLK1 also inhibited lumen formation, supporting its association with SASH1. In agreement with the role of DLK1 as a NOTCH1 inhibitor, NOTCH1, as well as its regulated genes HES1 and HEY1, were down-regulated in CEACAM1/MCF7 cells by the action of DLK1 RNAi, and up-regulated by SASH1 RNAi. When CEACAM1/MCF7 cells were treated with a γ-secretase inhibitor known to inhibit NOTCH signaling, lumen formation was inhibited. We conclude that restoration of lumen formation by CEACAM1 regulates the NOTCH1 signaling pathway via the adaptor protein SASH1 and the NOTCH1 inhibitor DLK1. These data suggest that the putative involvement of NOTCH1 as a tumor-promoting gene in breast cancer may depend on its lack of regulation in cancer, whereas its involvement in normal lumen formation requires activation of its expression, and subsequently, inhibition of its signaling. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. Modelagem da qualidade da água na bacia hidrográfica do Rio Piracicaba (MG

    Directory of Open Access Journals (Sweden)

    Maytê Maria Abreu Pires de Melo Silva

    Full Text Available RESUMO Este trabalho tem como objetivo aplicar um modelo de qualidade da água, o Sistema de Apoio à Decisão/Instituto de Pesquisas Hidráulicas (SAD-IPH, na bacia hidrográfica do Rio Piracicaba. Os parâmetros de qualidade modelados foram a demanda bioquímica de oxigênio (DBO, oxigênio dissolvido (OD, fósforo total, nitrogênio e coliformes termotolerantes. Os dados de vazão foram obtidos através da aplicação do modelo hidrológico Modelo de Grandes Bacias/IPH (MGB-IPH. O modelo de qualidade foi calibrado utilizando dados dos postos de monitoramento da qualidade da água do Instituto Mineiro de Gestão das Águas (IGAM. As curvas de DBO, OD e fósforo total apresentaram boa aderência aos dados das campanhas de monitoramento. Os parâmetros nitrogênio (orgânico, amoniacal, nitrito, nitrato e coliformes termotolerantes foram subestimados pelo modelo. A maioria dos cursos d’água apresentam a qualidade de suas águas compatíveis com a classe 4 de enquadramento, entretanto as concentrações poluentes atuais estão acima do esperado para a referida bacia, já que o seu enquadramento atual é classe 2.

  17. Hyphomycetes isolados da água e do solo da Reserva Florestal de Dois Irmãos, Recife, PE, Brasil

    OpenAIRE

    Cavalcanti,Marilene da Silva; Milanez,Adauto Ivo

    2007-01-01

    (Hyphomycetes isolados da água e do solo, da Reserva Florestal de Dois Irmãos, Recife, PE, Brasil). Visando ampliar o conhecimento sobre a diversidade de fungos em ambientes aquáticos, coletas da água e do solo das margens dos açudes do Vale do Prata e do Meio foram efetuadas na Reserva Florestal de Dois Irmãos, Recife, Estado de Pernambuco. Dentre outras, foram isoladas cinco espécies pouco comuns de Hyphomycetes. As amostras de água foram coletadas abaixo da lâmina d'água e as de solo nas m...

  18. Genes Involved in Human Ribosome Biogenesis areTranscriptionally Upregulated in Colorectal Cancer

    DEFF Research Database (Denmark)

    Mansilla, Francisco; Lamy, Philippe; Ørntoft, Torben Falck

    2009-01-01

    Microarray gene expression profiling comprising 168 colorectal adenocarcinomas and 10 normal mucosas showed that over 79% of the genes involved in human ribosome biogenesis are significantly upregulated (log2>0.5, p<10-3) when compared to normal mucosa. Overexpression was independent of microsate......Microarray gene expression profiling comprising 168 colorectal adenocarcinomas and 10 normal mucosas showed that over 79% of the genes involved in human ribosome biogenesis are significantly upregulated (log2>0.5, p... of microsatellite status. The promoters of the genes studied showed a significant enrichment for several transcription factor binding sites. There was a significant correlation between the number of binding site targets for these transcription factors and the observed gene transcript upregulation. The upregulation...

  19. A pomegranate (Punica granatum L.) WD40-repeat gene is a functional homologue of Arabidopsis TTG1 and is involved in the regulation of anthocyanin biosynthesis during pomegranate fruit development.

    Science.gov (United States)

    Ben-Simhon, Zohar; Judeinstein, Sylvie; Nadler-Hassar, Talia; Trainin, Taly; Bar-Ya'akov, Irit; Borochov-Neori, Hamutal; Holland, Doron

    2011-11-01

    Anthocyanins are the major pigments responsible for the pomegranate (Punica granatum L.) fruit skin color. The high variability in fruit external color in pomegranate cultivars reflects variations in anthocyanin composition. To identify genes involved in the regulation of anthocyanin biosynthesis pathway in the pomegranate fruit skin we have isolated, expressed and characterized the pomegranate homologue of the Arabidopsis thaliana TRANSPARENT TESTA GLABRA1 (TTG1), encoding a WD40-repeat protein. The TTG1 protein is a regulator of anthocyanins and proanthocyanidins (PAs) biosynthesis in Arabidopsis, and acts by the formation of a transcriptional regulatory complex with two other regulatory proteins: bHLH and MYB. Our results reveal that the pomegranate gene, designated PgWD40, recovered the anthocyanin, PAs, trichome and seed coat mucilage phenotype in Arabidopsis ttg1 mutant. PgWD40 expression and anthocyanin composition in the skin were analyzed during pomegranate fruit development, in two accessions that differ in skin color intensity and timing of appearance. The results indicate high positive correlation between the total cyanidin derivatives quantity (red pigments) and the expression level of PgWD40. Furthermore, strong correlation was found between the steady state levels of PgWD40 transcripts and the transcripts of pomegranate homologues of the structural genes PgDFR and PgLDOX. PgWD40, PgDFR and PgLDOX expression also correlated with the expression of pomegranate homologues of the regulatory genes PgAn1 (bHLH) and PgAn2 (MYB). On the basis of our results we propose that PgWD40 is involved in the regulation of anthocyanin biosynthesis during pomegranate fruit development and that expression of PgWD40, PgAn1 and PgAn2 in the pomegranate fruit skin is required to regulate the expression of downstream structural genes involved in the anthocyanin biosynthesis.

  20. POLYGALACTURONASE INVOLVED IN EXPANSION1 functions in cell elongation and flower development in Arabidopsis.

    Science.gov (United States)

    Xiao, Chaowen; Somerville, Chris; Anderson, Charles T

    2014-03-01

    Pectins are acidic carbohydrates that comprise a significant fraction of the primary walls of eudicotyledonous plant cells. They influence wall porosity and extensibility, thus controlling cell and organ growth during plant development. The regulated degradation of pectins is required for many cell separation events in plants, but the role of pectin degradation in cell expansion is poorly defined. Using an activation tag screen designed to isolate genes involved in wall expansion, we identified a gene encoding a putative polygalacturonase that, when overexpressed, resulted in enhanced hypocotyl elongation in etiolated Arabidopsis thaliana seedlings. We named this gene POLYGALACTURONASE INVOLVED IN EXPANSION1 (PGX1). Plants lacking PGX1 display reduced hypocotyl elongation that is complemented by transgenic PGX1 expression. PGX1 is expressed in expanding tissues throughout development, including seedlings, roots, leaves, and flowers. PGX1-GFP (green fluorescent protein) localizes to the apoplast, and heterologously expressed PGX1 displays in vitro polygalacturonase activity, supporting a function for this protein in apoplastic pectin degradation. Plants either overexpressing or lacking PGX1 display alterations in total polygalacturonase activity, pectin molecular mass, and wall composition and also display higher proportions of flowers with extra petals, suggesting PGX1's involvement in floral organ patterning. These results reveal new roles for polygalacturonases in plant development.

  1. Aproveitamento de águas de chuva por telhados: aspectos quantitativos e qualitativos

    OpenAIRE

    Farias, Maria Mariah Monteiro Wanderley Estanislau Costa de

    2012-01-01

    Nos últimos anos tem-se observado, em várias localidades, um crescimento populacional acelerado. A maior parte da população vive em áreas urbanas e enfrenta diversos problemas relacionados ao abastecimento precário de água e à ocorrência de eventos extremos, tais como estiagem prolongada e enchentes. Nesse contexto, se insere o aproveitamento de águas de chuva, que deverão ser captadas e acumuladas em reservatórios, de modo que a mesma possa ser consumida, de imediato ou após algum tempo, em ...

  2. The c.IVS1+1G>A mutation inthe GJB2 gene is prevalent and large ...

    Indian Academy of Sciences (India)

    IVS1+1G>A mutation inthe GJB2 gene is prevalent and large deletions involving the GJB6 gene are not present in the Turkish population. ASLI SIRMACI, DUYGU AKCAYOZ-DUMAN and MUSTAFA TEKIN∗. Division of Pediatric Molecular Genetics, Ankara University School of Medicine, Ankara 06100, Turkey. Introduction.

  3. Genes affecting β-cell function in type 1 diabetes

    DEFF Research Database (Denmark)

    Fløyel, Tina; Kaur, Simranjeet; Pociot, Flemming

    2015-01-01

    Type 1 diabetes (T1D) is a multifactorial disease resulting from an immune-mediated destruction of the insulin-producing pancreatic β cells. Several environmental and genetic risk factors predispose to the disease. Genome-wide association studies (GWAS) have identified around 50 genetic regions...... that affect the risk of developing T1D, but the disease-causing variants and genes are still largely unknown. In this review, we discuss the current status of T1D susceptibility loci and candidate genes with focus on the β cell. At least 40 % of the genes in the T1D susceptibility loci are expressed in human...... islets and β cells, where they according to recent studies modulate the β-cell response to the immune system. As most of the risk variants map to noncoding regions of the genome, i.e., promoters, enhancers, intergenic regions, and noncoding genes, their possible involvement in T1D pathogenesis as gene...

  4. Thioridazine affects transcription of genes involved in cell wall biosynthesis in methicillin-resistant Staphylococcus aureus

    DEFF Research Database (Denmark)

    Bonde, Mette; Højland, Dorte Heidi; Kolmos, Hans Jørn

    2011-01-01

    have previously shown that the expression of some resistance genes is abolished after treatment with thioridazine and oxacillin. To further understand the mechanism underlying the reversal of resistance, we tested the expression of genes involved in antibiotic resistance and cell wall biosynthesis...... in response to thioridazine in combination with oxacillin. We observed that the oxacillin-induced expression of genes belonging to the VraSR regulon is reduced by the addition of thioridazine. The exclusion of such key factors involved in cell wall biosynthesis will most likely lead to a weakened cell wall...... reversal of resistance by thioridazine relies on decreased expression of specific genes involved in cell wall biosynthesis....

  5. Involvement of co-repressor LUH and the adapter proteins SLK1 and SLK2 in the regulation of abiotic stress response genes in Arabidopsis.

    Science.gov (United States)

    Shrestha, Barsha; Guragain, Bhuwan; Sridhar, Vaniyambadi V

    2014-02-24

    During abiotic stress many genes that are important for growth and adaptation to stress are expressed at elevated levels. However, the mechanisms that keep the stress responsive genes from expressing under non stress conditions remain elusive. Recent genetic characterization of the co-repressor LEUNIG_HOMOLOG (LUH) and transcriptional adaptor proteins SEUSS-LIKE1 (SLK1) and SLK2 have been proposed to function redundantly in diverse developmental processes; however their function in the abiotic stress response is unknown. Moreover, the molecular functions of LUH, SLK1 and SLK2 remain obscure. Here, we show the molecular function of LUH, SLK1 and SLK2 and the role of this complex in the abiotic stress response. The luh, slk1 and slk2 mutant plants shows enhanced tolerance to salt and osmotic stress conditions. SLK1 and SLK2 interact physically with the LUFS domain in LUH forming SLK1-LUH and SLK2-LUH co-repressor complexes to inhibit the transcription. LUH has repressor activity, whereas SLK1 and SLK2 function as adaptors to recruit LUH, which in turn recruits histone deacetylase to the target sequences to repress transcription. The stress response genes RD20, MYB2 and NAC019 are expressed at elevated levels in the luh, slk1 and slk2 mutant plants. Furthermore, these stress response genes are associated with decreased nucleosome density and increased acetylation levels at H3K9 and H3K14 in the luh, slk1 and slk2 mutant plants. Our results indicate that SLK1, SLK2 and LUH form a co-repressor complex. LUH represses by means of an epigenetic process involving histone modification to facilitate the condensation of chromatin thus preventing transcription at the target genes.

  6. TAXA DE GESTAÇÃO EM ÉGUAS DA RAÇA CRIOULA APÓS ASPIRAÇÃO FOLICULAR GUIADA POR ULTRASSOM

    Directory of Open Access Journals (Sweden)

    Vivian Campos Laia Franco

    2014-09-01

    Full Text Available Há poucos estudos sobre aspiração folicular transvaginal guiada por ultrassom na medicina equina abordando complicações futuras na fertilidade das éguas aspiradas. Com o objetivo de avaliar o efeito da aspiração folicular na fertilidade das éguas, foram conduzidos dois experimentos. No experimento I, 15 éguas da raça Crioula foram distribuídas em três grupos de acordo com o diâmetro do folículo aspirado durante o estro: 25-29mm (n=4; grupo 1; 30-34mm (n=6; grupo 2;  35mm (n=5; grupo 3 e grupo controle (n=15; grupo 4. No experimento II, a aspiração folicular foi realizada em 25 éguas durante o diestro quando pelo menos 4 folículos (>5mm foram observados na ultrassonografia transretal em ambos os ovários. Foram aspirados todos os folículos visíveis, entre 4 e 8 mm. Trinta e uma éguas serviram como controle. No experimento I, a taxa de prenhez no ciclo seguinte a aspiração foi de 75% (grupo 1, 83,3% (grupo 2, 60% (grupo 3, e 73,3% (grupo 4. No experimento II foi de 76% no grupo aspirado e 77,4% no grupo controle (não aspirado. Em ambos os experimentos, as taxas de prenhez foram similares (P>0,05. Os resultados mostram que a taxa de concepção no primeiro ciclo após a aspiração folicular não é afetada pelo procedimento.

  7. Diminuição do pico de cheia na rede de drenagem de águas pluviais através do sistema de aproveitamento de água da chuva

    OpenAIRE

    Pomares, Rui Miguel Cândido

    2010-01-01

    Dissertação de mest., Engenharia de Recursos Hídricos, Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2010 A água tem-se tornado um recurso da maior importância, devido não só ao crescimento demográfico, mas fundamentalmente, ao desenvolvimento económico e ao nosso estilo de vida. A água potável é hoje um recurso a preservar que de bem comunitário e patrimonial, se transformou ao longo das últimas décadas num bem económico. De acordo com as previsões do World Water Counc...

  8. Characterization of the ptr5+ gene involved in nuclear mRNA export in fission yeast

    International Nuclear Information System (INIS)

    Watanabe, Nobuyoshi; Ikeda, Terumasa; Mizuki, Fumitaka; Tani, Tokio

    2012-01-01

    Highlights: ► We cloned the ptr5 + gene involved in nuclear mRNA export in fission yeast. ► The ptr5 + gene was found to encode nucleoporin 85 (Nup85). ► Seh1p and Mlo3p are multi-copy suppressors for the ptr5 mutation. ► Ptr5p/Nup85p functions in nuclear mRNA export through the mRNA export factor Rae1p. ► Ptr5p/Nup85p interacts genetically with pre-mRNA splicing factors. -- Abstract: To analyze the mechanisms of mRNA export from the nucleus to the cytoplasm, we have isolated eleven mutants, ptr [poly(A) + RNA transport] 1 to 11, which accumulate poly(A) + RNA in the nucleus at a nonpermissive temperature in Schizosaccharomyces pombe. Of those, the ptr5–1 mutant shows dots- or a ring-like accumulation of poly(A) + RNA at the nuclear periphery after shifting to the nonpermissive temperature. We cloned the ptr5 + gene and found that it encodes a component of the nuclear pore complex (NPC), nucleoporin 85 (Nup85). The ptr5–1 mutant shows no defects in protein transport, suggesting the specific involvement of Ptr5p/Nup85p in nuclear mRNA export in S. pombe. We identified Seh1p, a nucleoporin interacting with Nup85p, an mRNA-binding protein Mlo3p, and Sac3p, a component of the TREX-2 complex involved in coupling of nuclear mRNA export with transcription, as multi-copy suppressors for the ptr5–1 mutation. In addition, we found that the ptr5–1 mutation is synthetically lethal with a mutation of the mRNA export factor Rae1p, and that the double mutant exaggerates defective nuclear mRNA export, suggesting that Ptr5p/Nup85p is involved in nuclear mRNA export through Rae1p. Interestingly, the ptr5–1 mutation also showed synthetic effects with several prp pre-mRNA splicing mutations, suggesting a functional linkage between the NPCs and the splicing apparatus in the yeast nucleus.

  9. Água, saúde e territórios: uma breve discussão

    Directory of Open Access Journals (Sweden)

    Helen C. Gurgel

    2013-12-01

    Full Text Available A água e o meio ambiente, recursos vitais, interagemde várias maneiras com a saúde humana:vetor de agente microbiano ou tóxicos,ambiente de vida de vetores biológicos ou aindaimportante meio de favorecimento de desenvolvimentode epidemias durante e após eventosextremos. Todas essas dimensões de interaçãoentre a água e a saúde humana são tema depolíticas públicas diferenciadas, em escalasque variam da supranacional ao local nos territóriosque se apresentam hora como locais deapropriação e de vida e hora como lugar de expressãodas políticas. Após uma apresentaçãodas principais doenças de maior preocupaçãopara a saúde pública global relacionada à água(amebíase (diarreia, esquistossomose e malária,apresentamos a situação mais específicado território brasileiro, principalmente no quediz respeito à diarreia infantil, a contaminaçãopor cianobactérias e as doenças vetoriais: malária,dengue, esquistossomose e leptospirose,apresentamos algumas medidas recentementeimplementadas pelo governo brasileiro para tentarsuplantar as questões ligadas à relação entreágua e a saúde.

  10. Microcistinas: risco de contaminação em águas eutróficas

    Directory of Open Access Journals (Sweden)

    Marcia Kamogae

    2000-05-01

    Full Text Available O aumento populacional, aliado à modernização da sociedade, incrementou o consumo de água, tornando o represamento recurso imprescindível para atender à demanda. Inúmeros fatores inter-relacionados contribuem para agravar o risco da deterioração da água, dentre os quais se destacam as microcistinas. Estas hepatotoxinas, produzidas por cianobactérias dos gêneros Anabaena, Microcystis, Nostoc e Oscillatorias, consistem de heptapeptídio de estrutura monocíclica cujo risco iminente da constante ingestão se deve ao efeito de potente promotor de tumor. A produção de microcistinas é favorecida por fatores ambientais onde existe elevada concentração de nutrientes e luminosidade, fatores estes que se tornaram inevitáveis no atual ecossistema. A crescente eutroficação de água ocorre em ritmo acelerado, decorrente da poluição pela industrialização, lixo doméstico, práticas agrícolas, além da espoliação de recursos naturais. Com a finalidade de alertar a comunidade e despertar seu interesse, este trabalho discorre sobre microcistinas e fatores de risco - perspectivas de sua contaminação, objetivando ainda estabelecer medidas preventivas que minimizem a deterioração das águas.

  11. Gene-environment interactions involving functional variants

    DEFF Research Database (Denmark)

    Barrdahl, Myrto; Rudolph, Anja; Hopper, John L

    2017-01-01

    .36, 95% CI: 1.16-1.59, pint  = 1.9 × 10(-5) ) in relation to ER- disease risk. The remaining two gene-environment interactions were also identified in relation to ER- breast cancer risk and were found between 3p21-rs6796502 and age at menarche (ORint  = 1.26, 95% CI: 1.12-1.43, pint =1.8 × 10...... epidemiological breast cancer risk factors in relation to breast cancer. Analyses were conducted on up to 58,573 subjects (26,968 cases and 31,605 controls) from the Breast Cancer Association Consortium, in one of the largest studies of its kind. Analyses were carried out separately for estrogen receptor (ER......) positive (ER+) and ER negative (ER-) disease. The Bayesian False Discovery Probability (BFDP) was computed to assess the noteworthiness of the results. Four potential gene-environment interactions were identified as noteworthy (BFDP 

  12. Differential transactivation by orphan nuclear receptor NOR1 and its fusion gene product EWS/NOR1: possible involvement of poly(ADP-ribose) polymerase I, PARP-1.

    Science.gov (United States)

    Ohkura, Naganari; Nagamura, Yuko; Tsukada, Toshihiko

    2008-10-15

    In extraskeletal myxoid chondrosarcoma, a chromosomal translocation creates a gene fusion between EWS and an orphan nuclear receptor, NOR1. The resulting fusion protein EWS/NOR1 has been believed to lead to malignant transformation by functioning as a transactivator for NOR1-target genes. By comparing the gene expression profiles of NOR1- and EWS/NOR1-overexpressing cells, we found that they largely shared up-regulated genes, but no significant correlation was observed with respect to the transactivation levels of each gene. In addition, the proteins associated with NOR1 and EWS/NOR1 were mostly the same in these cells. The results suggest that these proteins differentially transactivate overlapping target genes through a similar transcriptional machinery. To clarify the mechanisms underlying the transcriptional divergence between NOR1 and EWS/NOR1, we searched for alternatively associated proteins, and identified poly(ADP-ribose) polymerase I (PARP-1) as an NOR1-specific binding protein. Consistent with its binding properties, PARP-1 acted as a transcriptional repressor of NOR1, but not EWS/NOR1, in a luciferase reporter assay employing PARP-1(-/-) fibroblasts. Interestingly, suppressive activity of PARP-1 was observed in a DNA response element-specific manner, and in a subtype-specific manner toward the NR4A family (Nur77, Nurr1, and NOR1), suggesting that PARP-1 plays a role in the diversity of transcriptional regulation mediated by the NR4A family in normal cells. Altogether, our findings suggest that NOR1 and EWS/NOR1 regulate overlapping target genes differently by utilizing associated proteins, including PARP-1; and that EWS/NOR1 may acquire oncogenic activities by avoiding (or gaining) transcription factor-specific modulation by the associated proteins. (c) 2008 Wiley-Liss, Inc.

  13. Possible gene dosage effect of glutathione-S-transferases on atopic asthma: using real-time PCR for quantification of GSTM1 and GSTT1 gene copy numbers

    DEFF Research Database (Denmark)

    Brasch-Andersen, Charlotte; Christiansen, L; Tan, Q

    2004-01-01

    -S-transferase (GST) involved in the antioxidant defense were tested for association to asthma using 246 Danish atopic families in a family-based transmission disequilibrium test (TDT) design. A real-time PCR assay for relative quantification of gene copy number of GSTM1 and GSTT1 was developed. The assay made......Asthma is a complex genetic disorder characterized by chronic inflammation in the airways. As oxidative stress is a key component of inflammation, variations in genes involved in antioxidant defense could therefore be likely candidates for asthma. Three enzymes from the superfamily glutathione...

  14. Mechanisms Involved in Acquisition of blaNDM Genes by IncA/C2 and IncFIIY Plasmids.

    Science.gov (United States)

    Wailan, Alexander M; Sidjabat, Hanna E; Yam, Wan Keat; Alikhan, Nabil-Fareed; Petty, Nicola K; Sartor, Anna L; Williamson, Deborah A; Forde, Brian M; Schembri, Mark A; Beatson, Scott A; Paterson, David L; Walsh, Timothy R; Partridge, Sally R

    2016-07-01

    blaNDM genes confer carbapenem resistance and have been identified on transferable plasmids belonging to different incompatibility (Inc) groups. Here we present the complete sequences of four plasmids carrying a blaNDM gene, pKP1-NDM-1, pEC2-NDM-3, pECL3-NDM-1, and pEC4-NDM-6, from four clinical samples originating from four different patients. Different plasmids carry segments that align to different parts of the blaNDM region found on Acinetobacter plasmids. pKP1-NDM-1 and pEC2-NDM-3, from Klebsiella pneumoniae and Escherichia coli, respectively, were identified as type 1 IncA/C2 plasmids with almost identical backbones. Different regions carrying blaNDM are inserted in different locations in the antibiotic resistance island known as ARI-A, and ISCR1 may have been involved in the acquisition of blaNDM-3 by pEC2-NDM-3. pECL3-NDM-1 and pEC4-NDM-6, from Enterobacter cloacae and E. coli, respectively, have similar IncFIIY backbones, but different regions carrying blaNDM are found in different locations. Tn3-derived inverted-repeat transposable elements (TIME) appear to have been involved in the acquisition of blaNDM-6 by pEC4-NDM-6 and the rmtC 16S rRNA methylase gene by IncFIIY plasmids. Characterization of these plasmids further demonstrates that even very closely related plasmids may have acquired blaNDM genes by different mechanisms. These findings also illustrate the complex relationships between antimicrobial resistance genes, transposable elements, and plasmids and provide insights into the possible routes for transmission of blaNDM genes among species of the Enterobacteriaceae family. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  15. Combinação de águas doce e salobra para produção de alface hidropônica Combination of fresh and brackish waters for hydroponic lettuce production

    Directory of Open Access Journals (Sweden)

    Tales M. Soares

    2010-07-01

    Full Text Available Plantas de alface crespa cv. Verônica foram cultivadas em condição hidropônica objetivando-se avaliar dois tipos de emprego combinado de águas doce e salobras, quais sejam: usar as águas salobras para preparar a solução nutritiva (SN e a água doce para repor a evapotranspiração da cultura (ETc (Experimento I e usar a água doce para preparar a SN e as salobras para a reposição da ETc (Experimento II. Uma estrutura de pesquisa com 40 parcelas que simulam a técnica do fluxo laminar de nutrientes (NFT foi construída. Os níveis de salinidade da água testados foram: 0,585; 1,17; 1,755; 2,925 e 4,095 g L-1 de NaCl. A salinidade diminuiu a produtividade da alface (massa de matéria fresca da parte aérea em 10,9 e 3,91% para cada acréscimo unitário na salinidade da água (g L-1 de NaCl, considerando-se os Experimentos I e II, respectivamente. De acordo com os resultados obtidos com os dois tipos de combinação de águas doce e salobras, o uso de águas salobras para repor a ETc pode aumentar a produtividade da alface em comparação com o uso dessas águas para preparar a SN.Plants of crisphead lettuce cv. Verônica were grown under hydroponic conditions aiming to evaluate two types of combination of brackish and fresh waters: using the brackish waters to prepare nutrient solution (NS and fresh water to replace the evapotranspiration loss (ETc (Experiment I and using the fresh water to prepare NS and the brackish waters to replace the ETc loss (Experiment II. A research structure was built up with 40 experimental units which simulated the nutrient film technique (NFT. The levels of water salinity were 0.585; 1.17; 1.755; 2.925 and 4.095 g L-1 NaCl. The lettuce yield (fresh shoot matter decreased 10.9 and 3.91% for each unit increase in water salinity (g L-1 NaCl in Experiments I and II, respectively. According to results obtained with the two types of combination of fresh and brackish waters, the use of brackish waters to replace

  16. Identification of key genes involved in polysaccharide bioflocculant synthesis in Bacillus licheniformis.

    Science.gov (United States)

    Chen, Zhen; Liu, Peize; Li, Zhipeng; Yu, Wencheng; Wang, Zhi; Yao, Haosheng; Wang, Yuanpeng; Li, Qingbiao; Deng, Xu; He, Ning

    2017-03-01

    The present study reports the sequenced genome of Bacillus licheniformis CGMCC 2876, which is composed of a 4,284,461 bp chromosome that contains 4,188 protein-coding genes, 72 tRNA genes, and 21 rRNA genes. Additional analysis revealed an eps gene cluster with 16 open reading frames. Conserved Domains Database analysis combined with qPCR experiments indicated that all genes in this cluster were involved in polysaccharide bioflocculant synthesis. Phosphoglucomutase and UDP-glucose pyrophosphorylase were supposed to be key enzymes in polysaccharide secretion in B. licheniformis. A biosynthesis pathway for the production of polysaccharide bioflocculant involving the integration of individual genes was proposed based on functional analysis. Overexpression of epsDEF from the eps gene cluster in B. licheniformis CGMCC 2876 increased the flocculating activity of the recombinant strain by 90% compared to the original strain. Similarly, the crude yield of polysaccharide bioflocculant was enhanced by 27.8%. Overexpression of the UDP-glucose pyrophosphorylase gene not only increased the flocculating activity by 71% but also increased bioflocculant yield by 13.3%. Independent of UDP-N-acetyl-D-mannosamine dehydrogenase gene, flocculating activity, and polysaccharide yield were negatively impacted by overexpression of the UDP-N-acetylglucosamine 2-epimerase gene. Overall, epsDEF and gtaB2 were identified as key genes for polysaccharide bioflocculant synthesis in B. licheniformis. These results will be useful for further engineering of B. licheniformis for industrial bioflocculant production. Biotechnol. Bioeng. 2017;114: 645-655. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  17. Otimização de sistema de aquecimento solar de água em edificações residenciais unifamiliares utilizando o programa TRNSYS.

    OpenAIRE

    Juliana Benoni Arruda Lima

    2003-01-01

    Os sistemas de aquecimento solar de água têm sido amplamente utilizados no Brasil para uso doméstico em residências unifamiliares, aproveitando a energia potencial da água do reservatório superior e o fenômeno da termossifonagem para a circulação da água quente. Entretanto estes sistemas de aquecimento de água normalmente são dimensionados através de tabelas fornecidas pelos fabricantes que indicam o número de placas em função do tamanho da família e do número de pontos de utilização de água ...

  18. Crescimento de porta-enxerto de goiabeira sob águas salinizadas e doses de nitrogênio

    Directory of Open Access Journals (Sweden)

    Leandro de Pádua Souza

    2015-09-01

    Full Text Available A salinidade da água de irrigação pode afetar a exploração de cultivos principalmente nas regiões áridas e semiáridas, entretanto, algumas alternativas podem favorecer a exploração agrícola nestas condições. Desse modo, objetivou-se com este trabalho, avaliar a interação entre adubação nitrogenada e a salinidade da água de irrigação sob o crescimento de porta-enxerto de goiabeira ‘Crioula’ em condição de semiárido do nordeste do Brasil. O experimento foi conduzido em tubetes sob condições de ambiente protegido no CCTA/UFCG. Utilizou-se o delineamento experimental em blocos casualizados, com os tratamentos dispostos em esquema fatorial 5 x 4, correspondentes a  cinco níveis de condutividade elétrica da água – CEa (0,3; 1,1; 1,9; 2,7 e 3,5 dS m-1 e quatro doses de nitrogênio (70, 100, 130 e 160% de N da dose recomendada para a cultura, com 4 repetições e 5 plantas por parcela. A dose de 70% de N (541,1 mg de N dm-1 de solo proporciona maior diâmetro de caule de porta-enxerto de goiabeira crioula aos 70 e 145 dias após a emergência (DAE, sobre a área foliar e altura de planta aos 145 DAE e na taxa de crescimento absoluto diâmetro do caule dos 25 a 145 DAE. A irrigação com água cuja salinidade era acima de 0,3 dS m-1 afeta positivamente a razão de área foliar e de forma negativa, o número de folhas, a área foliar e o diâmetro do caule. Houve interação significativa entre os fatores adubação nitrogenada e salinidade da água de irrigação apenas para a variável número de folhas aos 145 DAE.Guava rootstocks growth in salted water and nitrogen levelsAbstract: Salt stress suffered by plants due to concentration of irrigation water salts reduces the ability of exploitation of cultures especially in arid and semiarid regions. Thus, the aim of this study was to evaluate the effect of salinity of irrigation water on the growth of rootstock of guava 'Crioula' associated with increasing doses of

  19. The Gly482Ser genotype at the PPARGC1A gene and elevated blood pressure: a meta-analysis involving 13,949 individuals

    DEFF Research Database (Denmark)

    Vimaleswaran, Karani Santhanakrishnan; Luan, Jian'an; Andersen, Gitte

    2008-01-01

    The protein encoded by the PPARGC1A gene is expressed at high levels in metabolically active tissues and is involved in the control of oxidative stress via reactive oxygen species detoxification. Several recent reports suggest that the PPARGC1A Gly482Ser (rs8192678) missense polymorphism may relate...... inversely with blood pressure. We used conventional meta-analysis methods to assess the association between Gly482Ser and systolic (SBP) or diastolic blood pressures (DBP) or hypertension in 13,949 individuals from 17 studies, of which 6,042 were previously unpublished observations. The studies comprised...

  20. Estudo para proposta de critérios de qualidade da água para reúso urbano.

    OpenAIRE

    Vanessa Dias da Cunha

    2008-01-01

    A cada dia a disputa pelo uso da água entre os diversos setores aumenta gerando sérios conflitos entre os usuários. Para minimizar a crise da água, tem-se desenvolvido técnicas, estudos, projetos e medidas que visem a proteção e conservação dos recursos disponíveis. Neste contexto, a possibilidade de substituição de parte da água potável, para usos menos restritivos, por uma de qualidade inferior está cada vez mais em evidência. No entanto, a falta de regulamentação específica sobre o assunto...

  1. Marfan syndrome with a complex chromosomal rearrangement including deletion of the FBN1 gene

    Directory of Open Access Journals (Sweden)

    Colovati Mileny ES

    2012-01-01

    Full Text Available Abstract Background The majority of Marfan syndrome (MFS cases is caused by mutations in the fibrillin-1 gene (FBN1, mapped to chromosome 15q21.1. Only few reports on deletions including the whole FBN1 gene, detected by molecular cytogenetic techniques, were found in literature. Results We report here on a female patient with clinical symptoms of the MFS spectrum plus craniostenosis, hypothyroidism and intellectual deficiency who presents a 1.9 Mb deletion, including the FBN1 gene and a complex rearrangement with eight breakpoints involving chromosomes 6, 12 and 15. Discussion This is the first report of MFS with a complex chromosome rearrangement involving a deletion of FBN1 and contiguous genes. In addition to the typical clinical findings of the Marfan syndrome due to FBN1 gene haploinsufficiency, the patient presents features which may be due to the other gene deletions and possibly to the complex chromosome rearrangement.

  2. Effects of Gua-Sha therapy on breast engorgement: a randomized controlled trial.

    Science.gov (United States)

    Chiu, Jin-Yu; Gau, Meei-Ling; Kuo, Shu-Yu; Chang, Yung-Hsien; Kuo, Su-Chen; Tu, Hui-Chuan

    2010-03-01

    Breast engorgement is a common problem that affects the initiation and duration of breastfeeding. Limited solutions are available to relieve the discomfort associated with breast engorgement. Thus, further investigation of methods to achieve effective relief of symptoms is critical to promote breastfeeding success. : The purpose of this study was to determine the effects of two breast care methods, that is, scraping (Gua-Sha) therapy (administered to the experimental group) and traditional breast care (i.e., massage and heating; administered to the control group). A randomized controlled trial was conducted on 54 postpartum women at a Level III medical teaching hospital. Participant inclusion criteria included postpartum breastfeeding women (a) who had an uncomplicated delivery and (b) who were experiencing breast engorgement problems. The Gua-Sha protocol selected appropriate acupoint positions, which included ST16, ST18, SP17, and CV17. Each position was lightly scraped seven times in two cycles. For the control group, we used hot packs and massage for 20 min in accordance with recommendations given in an obstetrical technique textbook. Results showed no statistical differences between the two groups at baseline. Body temperature, breast temperature, breast engorgement, pain levels, and discomforting levels were statistically different between the two groups at 5 and 30 min after intervention (p account. Our findings provided empirical evidence supporting that Gua-Sha therapy may be used as an effective technique in the management of breast engorgement. By using Gua-Sha therapy, nurses can handle breast engorgement problems more effectively in primary care and hence help patients both physically and psychologically.

  3. Active coping of prenatally stressed rats in the forced swimming test: involvement of the Nurr1 gene.

    Science.gov (United States)

    Montes, Pedro; Ruiz-Sánchez, Elizabeth; Calvillo, Minerva; Rojas, Patricia

    2016-09-01

    Depending on genetic predisposition, prenatal stress may result in vulnerability or resilience to develop psychiatric disorders in adulthood. Nurr1 is an immediate early gene, important in the brain for the stress response. We tested the hypothesis that prenatal stress and the decrease of hippocampal Nurr1 alter offspring behavioral responses in the forced swimming test (FST). Pregnant Wistar rats were exposed to restraint stress (45 min, thrice daily) from gestation day 14. Prenatally stressed (PS) and non-prenatally stressed (NPS) male offspring were treated bilaterally with a Nurr1 antisense oligodeoxynucleotide (ODN; or control) into the hippocampus at 97 d of age. After 1 h, the rats were exposed to the FST (acute stressor) to analyze their behavioral responses. Thirty minutes after the FST, we analyzed the gene expression of Nurr1, Bdnf and Nr3c1 (genes for Nurr1, brain-derived neurotrophic factor (BDNF) and glucocorticoid receptor (GR), respectively) in the hippocampus, prefrontal cortex (PFC) and hypothalamus. Results showed that the decrease of hippocampal Nurr1 after the antisense ODN in adult NPS rats induces immobility (indicating depressive-like behavior). The PS adult rats, including the group with decreased hippocampal Nurr1, presented low immobility in the FST. This low immobility was concordant with maintenance of Nurr1 and Bdnf expression levels in the three analyzed brain regions; Nr3c1 gene expression was also maintained in the PFC and hypothalamus. These findings suggest that Nurr1 and associated genes could participate in the brain modifications induced by prenatal stress, allowing active coping (resilience) with acute stress in adulthood.

  4. Aspectos legais para a água de reúso

    Directory of Open Access Journals (Sweden)

    Rodrigo Gomes de Almeida

    2011-11-01

    Full Text Available No Brasil, a disponibilidade de água é abundante, por isso não existe por parte da população e do poder público uma preocupação em relação ao consumo racional deste recurso. A urbanização e industrialização crescentes geraram um problema de oferta e demanda de recursos hídricos. Uma das possíveis soluções para a resolução deste impasse é o reúso de águas residuárias. No entanto, é necessário regulamentar o reúso fazendo com que essa prática se desenvolva de acordo com princípios legais e técnicos adequados, de forma a assegurar a saúde do usuário e promover a preservação do meio ambiente.

  5. Effect of TPA and HTLV-1 Tax on BRCA1 and ERE controlled genes expression.

    Science.gov (United States)

    Jabareen, Azhar; Abu-Jaafar, Aya; Abou-Kandil, Ammar; Huleihel, Mahmoud

    2017-07-18

    Interference with the expression and/or functions of the multifunctional tumor suppressor BRCA1 leads to a high risk of breast and ovarian cancers. BRCA1 expression is usually activated by the estrogen (E2) liganded ERα receptor. Activated ERα is considered as a potent transcription factor which activates various genes expression by 2 pathways. A classical pathway, ERα binds directly to E2-responsive elements (EREs) in the promoters of the responsive genes and a non-classical pathway where ERα indirectly binds with the appropriate gene promoter. In our previous study, HTLV-1Tax was found to strongly inhibit ERα induced BRCA1 expression while stimulating ERα induced ERE dependent genes. TPA is a strong PKC activator which found to induce the expression of HTLV-1. Here we examined the effect of TPA on the expression of BRCA1 and genes controlled by ERE region in MCF-7 cells and on Tax activity on these genes. Our results showed strong stimulatory effect of TPA on both BRCA1 and ERE expression without treatment with E2. Tax did not show any significant effect on these TPA activities. It seems that TPA activation of BRCA1 and ERE expression is dependent on PKC activity but not through the NFκB pathway. However, 53BP1 may be involved in this TPA activity because its overexpression significantly reduced the TPA stimulatory effect on BRCA1 and ERE expression. Additionally, our Chip assay results probably exclude possible involvement of ERα pathway in this TPA activity because TPA did not interfere with the binding of ERα to both BRCA1 promoter and ERE region.

  6. Gu??a de autoempleo en Logopedia

    OpenAIRE

    Planells del Pozo, Elena Mar??a; Molina Cantero, Camila; Jacobe Mart??nez, Soledad; Jim??nez Rodr??guez, Jorge; Herrera Torres, Fuensanta

    2016-01-01

    Esta Gu??a de Autoempleo en Logopedia es un documento que pretende aportar informaci??n y generar motivaci??n al reci??n titulado en Logopedia, demostrando que dise??ar y crear un centro profesional propio es un camino posible y no supone tanta dificultad como se piensa. Este peque??o manual permitir?? recoger informaci??n dispersa referente al tema del autoempleo en Logopedia en un documento ??nico y asequible al titulado en Logopedia que se plantee y decida crear su propia empresa, apoya...

  7. Identification of genes involved in the biology of atypical teratoid/rhabdoid tumours using Drosophila melanogaster

    Science.gov (United States)

    Jeibmann, Astrid; Eikmeier, Kristin; Linge, Anna; Kool, Marcel; Koos, Björn; Schulz, Jacqueline; Albrecht, Stefanie; Bartelheim, Kerstin; Frühwald, Michael C.; Pfister, Stefan M.; Paulus, Werner; Hasselblatt, Martin

    2014-06-01

    Atypical teratoid/rhabdoid tumours (AT/RT) are malignant brain tumours. Unlike most other human brain tumours, AT/RT are characterized by inactivation of one single gene, SMARCB1. SMARCB1 is a member of the evolutionarily conserved SWI/SNF chromatin remodelling complex, which has an important role in the control of cell differentiation and proliferation. Little is known, however, about the pathways involved in the oncogenic effects of SMARCB1 inactivation, which might also represent targets for treatment. Here we report a comprehensive genetic screen in the fruit fly that revealed several genes not yet associated with loss of snr1, the Drosophila homologue of SMARCB1. We confirm the functional role of identified genes (including merlin, kibra and expanded, known to regulate hippo signalling pathway activity) in human rhabdoid tumour cell lines and AT/RT tumour samples. These results demonstrate that fly models can be employed for the identification of clinically relevant pathways in human cancer.

  8. Genes involved in cell division in mycoplasmas

    Directory of Open Access Journals (Sweden)

    Frank Alarcón

    2007-01-01

    Full Text Available Bacterial cell division has been studied mainly in model systems such as Escherichia coli and Bacillus subtilis, where it is described as a complex process with the participation of a group of proteins which assemble into a multiprotein complex called the septal ring. Mycoplasmas are cell wall-less bacteria presenting a reduced genome. Thus, it was important to compare their genomes to analyze putative genes involved in cell division processes. The division and cell wall (dcw cluster, which in E. coli and B. subtilis is composed of 16 and 17 genes, respectively, is represented by only three to four genes in mycoplasmas. Even the most conserved protein, FtsZ, is not present in all mycoplasma genomes analyzed so far. A model for the FtsZ protein from Mycoplasma hyopneumoniae and Mycoplasma synoviae has been constructed. The conserved residues, essential for GTP/GDP binding, are present in FtsZ from both species. A strong conservation of hydrophobic amino acid patterns is observed, and is probably necessary for the structural stability of the protein when active. M. synoviae FtsZ presents an extended amino acid sequence at the C-terminal portion of the protein, which may participate in interactions with other still unknown proteins crucial for the cell division process.

  9. Comparative Transcriptome Analysis Reveal Candidate Genes Potentially Involved in Regulation of Primocane Apex Rooting in Raspberry (Rubus spp.).

    Science.gov (United States)

    Liu, Jianfeng; Ming, Yuetong; Cheng, Yunqing; Zhang, Yuchu; Xing, Jiyang; Sun, Yuqi

    2017-01-01

    Raspberries ( Rubus spp.) exhibit a unique rooting process that is initiated from the stem apex of primocane, conferring an unusual asexual mode of reproduction to this plant. However, the full complement of genes involved in this process has not been identified. To this end, the present study analyzed the transcriptomes of the Rubus primocane and floricane stem apex at three developmental stages by Digital Gene Expression profiling to identify genes that regulate rooting. Sequencing and de novo assembly yielded 26.82 Gb of nucleotides and 59,173 unigenes; 498, 7,346, 4,110, 7,900, 9,397, and 4,776 differently expressed genes were identified in paired comparisons of SAF1 (floricane at developmental stage 1) vs. SAP1 (primocane at developmental stage 1), SAF2 vs. SAP2, SAF3 vs. SAP3, SAP1 vs. SAP2, SAP1 vs. SAP3, and SAP2 vs. SAP3, respectively. SAP1 maintains an extension growth pattern; SAP2 then exhibits growth arrest and vertical (downward) gravitropic deflection; and finally, short roots begin to form on the apex of SAP3. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis of SAP1 vs. SAP2 revealed 12 pathways that were activated in response to shoot growth arrest and root differentiation, including circadian rhythm-plant (ko04712) and plant hormone signal transduction (ko04075). Our results indicate that genes related to circadian rhythm, ethylene and auxin signaling, shoot growth, and root development are potentially involved in the regulation of primocane apex rooting in Rubus . These findings provide a basis for elucidating the molecular mechanisms of primocane apex rooting in this economically valuable crop.

  10. A R2R3-MYB transcription factor gene in common wheat (namely TaMYBsm1) involved in enhancement of drought tolerance in transgenic Arabidopsis.

    Science.gov (United States)

    Li, Meng-Jun; Qiao, Yu; Li, Ya-Qing; Shi, Zhan-Liang; Zhang, Nan; Bi, Cai-Li; Guo, Jin-Kao

    2016-11-01

    We isolated the TaMYBsm1 genes, encoding R2R3-type MYB proteins in common wheat, aimed to uncover the possible molecular mechanisms related to drought response. The TaMYBsm1 genes, TaMYBsm1-A, TaMYBsm1-B and TaMYBsm1-D, were isolated and analyzed from the common wheat cultivar Shimai 15. Their expression patterns under PEG 6000 and mannitol were monitored by semi-quantitative RT-PCR and β-glucuronidase (Gus) assay. The function of TaMYBsm1-D under drought stress in transgenic Arabidopsis plants was investigated, and the germination rate, water loss rate, as well as the proline and malondialdehyde (MDA) content were compared with that in wild type (WT) plants. The expression of three downstream genes (DREB2A, P5CS1 and RD29A) in TaMYBsm1-D transgenic plants was analyzed. The R2R3-MYB domains of the MYBsm1 proteins were highly conserved in plants. In addition, the TaMYBsm1 proteins were targeted to the nucleus and contained transcriptional activation domains (TADs). Gus assay and semi-quantitative RT-PCR analysis demonstrated that the TaMYBsm1 genes were up-regulated when the wheat was treated by PEG and mannitol. Compared with WT plants, the germination rates were much higher, but the water loss rates were much lower in TaMYBsm1-D overexpression plants. TaMYBsm1-D transgenic plants showed distinct higher proline contents but a lower MDA content than the WT plants. The three downstream genes were highly expressed in TaMYBsm1-D transgenic plants. We concluded from these results that TaMYBsm1 genes play an important role in plant drought stress tolerance through up-regulation of DREB2A, P5CS1 and RD29A. The increase of proline content and decrease of MDA content may also be involved in the drought response.

  11. Mutations in the SRY, DAX1, SF1 and WNT4 genes in Brazilian sex-reversed patients

    Directory of Open Access Journals (Sweden)

    S. Domenice

    2004-01-01

    Full Text Available In most mammals, male development is triggered by the transient expression of the SRY gene, which initiates a cascade of gene interactions ultimately leading to the formation of a testis from the indifferent fetal gonad. Mutation studies have identified several genes essential for early gonadal development. We report here a molecular study of the SRY, DAX1, SF1 and WNT4 genes, mainly involved in sexual determination, in Brazilian 46,XX and 46,XY sex-reversed patients. The group of 46,XX sex-reversed patients consisted of thirteen 46,XX true hermaphrodites and four 46,XX males, and was examined for the presence of the SRY gene and for the loss of function (inactivating mutations and deletions of DAX1 and WNT4 genes. In the second group consisting of thirty-three 46,XY sex-reversed patients we investigated the presence of inactivating mutations in the SRY and SF1 genes as well as the overexpression (duplication of the DAX1 and WNT4 genes. The SRY gene was present in two 46,XX male patients and in none of the true hermaphrodites. Only one mutation, located outside homeobox domain of the 5' region of the HMG box of SRY (S18N, was identified in a patient with 46,XY sex reversal. A novel 8-bp microdeletion of the SF1 gene was identified in a 46,XY sex-reversed patient without adrenal insufficiency. The dosage of DAX1 and WNT4 was normal in the sex-reversed patients studied. We conclude that these genes are rarely involved in the etiology of male gonadal development in sex-reversed patients, a fact suggesting the presence of other genes in the sex determination cascade.

  12. Germinação da vinagreira em função de cinco níveis de salinidade da água de irrigação

    Directory of Open Access Journals (Sweden)

    Mariana Alexandre de Lima Sales

    2014-04-01

    Full Text Available Normalmente, problemas de salinidade surgem quando os sais contidos na água de irrigação se acumulam na região do solo onde se encontra o sistema radicular das culturas e, com isto, seus rendimentos são afetados negativamente. Este ensaio teve por objetivo avaliar a germinação de vinagreira em função de cinco níveis de salinidade da água de irrigação. O trabalho foi realizado Laboratório de Análises de Solos e Água e em casa de vegetação, ambos localizados no IFCE, Campus de Sobral. No Ensaio I realizou-se testes preliminares que possibilitaram a construção de curvas artificiais de salinidade e no Ensaio II estudou-se os efeitos destes diferentes níveis de salinidade da água de irrigação, (T1 = testemunha – 0,17; T2=1,5; T3=2,5; T4=3,5; T5=4,5 e T6=5,5 dS m-1. As variáveis analisadas foram primeira contagem da germinação (PCG, porcentagem de germinação (%Germ, índice de velocidade de germinação (IVG e tempo médio de germinação (TMG. De posse dos resultados verifica-se que a elevação da condutividade elétrica da água de irrigação, reduziu o vigor da germinação das sementes de vinagreira; a condutividade elétrica do substrato apresentou aumento crescente com os níveis de salinidade da água de irrigação. Normal 0 21 false false false PT-BR X-NONE X-NONE

  13. Assignment of CSF-1 to 5q33.1: evidence for clustering of genes regulating hematopoiesis and for their involvement in the deletion of the long arm of chromosome 5 in myeloid disorders

    International Nuclear Information System (INIS)

    Pettenati, M.J.; Le Beau, M.M.; Lemons, R.S.; Shima, E.A.; Kawasaki, E.S.; Larson, R.A.; Sherr, C.J.; Diaz, M.O.; Rowley, J.D.

    1987-01-01

    The CSF-1 gene encodes a hematopoietic colony-stimulating factor (CSF) that promotes growth, differentiation, and survival of mononuclear phagocytes. By using somatic cell hybrids and in situ hybridization, the authors localized this gene to human chromosome 5 at bands q31 to q35, a chromosomal region that is frequently deleted [del(5q)] in patients with myeloid disorders. By in situ hybridization, the CSF-1 gene was found to be deleted in the 5q- chromosome of a patient with refractory anemia who had a del(5) (q15q33.3) and in that of a second patient with acute nonlymphocytic leukemia de novo who had a similar distal breakpoint [del(5)(q13q33.3)]. The gene was present in the deleted chromosome of a third patient, with therapy-related acute nonlymphocytic leukemia, who had a more proximal breakpoint in band q33 [del(5)(q22q33.1)]. Hybridization of the CSF-1 probe to metaphase cells of a fourth patient, with acute nonlymphocytic leukemia de novo, who had a rearrangement of chromosomes 5 and 21 resulted in labeling of the breakpoint junctions of both rearranged chromosomes; this suggested that CSF-1 is located at 5q33.1. Thus, a small segment of chromosome 5 contains GM-CSF (the gene encoding the granulocyte-macrophage CSF), CSF-1, and FMS, which encodes the CSF-1 receptor, in that order from the centromere; this cluster of genes may be involved in the altered hematopoiesis associated with a deletion of 5q

  14. Pore size distribution in soils irrigated with sodic water and wastewater Distribuição de poros em solos irrigados com água salina e com água residuária

    Directory of Open Access Journals (Sweden)

    Roberta Alessandra Bruschi Gonçalves

    2010-06-01

    Full Text Available Soil porosity, especially pore size distribution, is an important controlling factor for soil infiltration, hydraulic conductivity, and water retention. This study aimed to verify the effect of secondary-treated domestic wastewater (STW on the porosity of a sandy loam Oxisol in the city of Lins, state of São Paulo, Brazil. The two-year experiment was divided into three plots: soil cultivated with corn and sunflower and irrigated with STW, soil cultivated and irrigated with sodic groundwater, and non-irrigated and non-cultivated soil (control. At the end of the experiment, undisturbed core samples were sampled from 0 to 2.0 m (8 depths. The water retention curves were obtained by tension plates and Richard's pressure plate apparatus, and the pore size distribution inferred from the retention curves. It was found that irrigation with treated wastewater and treated groundwater led to a decrease in microporosity (V MI, defined as the pore class ranging from 0.2 to 50 μm diameter. On the other hand, a significant increase in cryptoporosity (V CRI (A porosidade do solo, principalmente a distribuição dos poros, é um fator importante que controla a infiltração de água, condutividade hidráulica e retenção da água no solo. Este estudo teve como objetivo verificar os efeitos do efluente de estação de tratamento de esgoto (TSE na porosidade de um Latossolo de textura média. A área experimental foi dividida em três parcelas: solo cultivado com milho e girassol e irrigado com TSE (STW; solo cultivado e irrigado com água subterrânea sódica (W; e solo não cultivado e não irrigado (C-controle. No final de dois anos de experimento, amostras não deformadas de solo foram coletadas de 0 a 2,0 m (oito amostras. As curvas de retenção de água no solo foram obtidas com mesas de tensão e câmara de Richards, e a distribuição de poros no solo foi calculada a partir da derivação dessas curvas. Foi observado decréscimo da microporosidade V MI

  15. ANÁLISE DAS PRÁTICAS DE REUSO DE ÁGUA RESIDUAL: ESTUDO DE CASOS EM LAVANDERIAS INDUSTRIAIS

    OpenAIRE

    Souza, Mariane Cásseres; Vargas, Andrea; Saueressig, Gislaine Gabriele; Luchese, Juliane; Bauer, Jéssica Mariella; Viegas, Cláudia Viviane

    2016-01-01

    Água é um insumo de uso abundante em processos de lavanderias industriais. O reuso da água em Estação de Tratamento de Efluente reduz custos e traz benefícios ambientais, ou seja, é um fator de ecoeficiência. Esta pesquisa analisa duas empresas do ramo de lavanderias industriais, de médio porte, localizadas na região Sul do Brasil, e identifica melhorias quanto ao reúso da água. O método de pesquisa foi o estudo de caso múltiplo. Os resultados obtidos identificaram a diminuição da carga polui...

  16. Analysis of BRCA1 involvement in breast cancer in Indian women

    Indian Academy of Sciences (India)

    The involvement of the familial breast-ovarian cancer gene (BRCA1) in the molecular pathogenesis of breast cancer among Indian women is unknown. We have used a set of microsatellite polymorphisms to examine the frequency of allele loss at the BRCA1 region on chromosome 17q21, in a panel of 80 human breast ...

  17. Role of NPR1 dependent and NPR1 independent genes in response to Salicylic acid

    Directory of Open Access Journals (Sweden)

    Neha Agarwal

    2017-10-01

    Full Text Available NPR1 (Nonexpressor of pathogenesis-related gene is a transcription coactivator and central regulator of systemic acquired resistance (SAR pathway. It controls wide range of pathogenesis related genes involved in various defense responses, acts by sensing SAR signal molecule, Salicylic acid (SA. Mutation in NPR1 results in increased susceptibility to pathogen infection and less expression of pathogenesis related genes. The present study aimed to identify the role of NPR1 in gene expression after the Salicylic acid induction. For this RNA-seq was performed in Arabidopsis thaliana Col-0 and npr1-1 in response to Salicylic acid. RNA-seq analysis revealed a total of 3811 differentially expressed gene in which 2109 genes are up-regulated and 1702 genes are down-regulated. We have divided these genes in 6 categories SA induced (SI, SA repressed (SR, NPR1 dependent SI (ND-SI, NPR1 dependent SR (ND-SR, NPR1 independent SI (NI-SI, NPR1 independent SR (NI-SR. Further, Gene ontology and MapMan pathway analysis of differentially expressed genes suggested variety of biological processes and metabolic pathways that are enriched during SAR defense pathway. These results contribute to shed light on importance of both NPR1-dependent (ND and NPR1-independent (NI gene acting downstream to Salicylic acid induction in SAR pathway. The present study aimed to identify the role of NPR1 in gene expression after the Salicylic acid induction.

  18. AVALIAÇAO DA QUALIDADE DAS ÁGUAS SUBTERRÂNEAS DA BACIA HIDROGRÁFICA DO RIO PARDO, SISTEMA AQUÍFERO GUARANI, RS, BRASIL

    Directory of Open Access Journals (Sweden)

    Marluce Purper

    2011-08-01

    Full Text Available O objetivo desta pesquisa foi avaliar a qualidade das águas subterrâneas em áreas de preservação permanente (Sistema Aquífero Guarani – SAG da Bacia Hidrográfica do Rio Pardo, RS, Brasil, através de variáveis físicas, químicas e microbiológicas. Nove pontos de coleta foram distribuídos ao longo da bacia, nos quais as águas subterrâneas foram classificadas quanto aos íons de maior ocorrência quantitativa. Os resultados indicaram que a maioria dos poços avaliados enquadraram-se na Classe 4 de usos da água, correspondendo a águas de usos menos restritivos (como recreação de contato secundário. Entretanto, deve-se considerar que os aquíferos são caracterizados por diferentes condições geológicas, com características físicas, químicas e biológicas intrínsecas, e também variações hidrogeoquímicas, sendo necessário que as suas classes de qualidade sejam determinadas com base nessas especificidades. As amostras P1, P2, P3, P5 e P9 classificaram-se como bicarbonatadas cálcicas; as águas dos pontos P4, P6, P7 como bicarbonatadas sódicas; e P8 como sulfatada. Verificou-se que a qualidade das águas de poços com profundidade inferior a 6 m está mais vulnerável, devido a alterações antrópicas em função da concentração de nitrato, coliformes totais e termotolerantes, enquanto a qualidade das águas de poços mais profundos depende basicamente de suas características hidrogeológicas e hidrogeoquímicas naturais, em função das variáveis sulfato e sódio.

  19. ESTs analysis reveals putative genes involved in symbiotic seed germination in Dendrobium officinale.

    Science.gov (United States)

    Zhao, Ming-Ming; Zhang, Gang; Zhang, Da-Wei; Hsiao, Yu-Yun; Guo, Shun-Xing

    2013-01-01

    Dendrobiumofficinale (Orchidaceae) is one of the world's most endangered plants with great medicinal value. In nature, D. officinale seeds must establish symbiotic relationships with fungi to germinate. However, the molecular events involved in the interaction between fungus and plant during this process are poorly understood. To isolate the genes involved in symbiotic germination, a suppression subtractive hybridization (SSH) cDNA library of symbiotically germinated D. officinale seeds was constructed. From this library, 1437 expressed sequence tags (ESTs) were clustered to 1074 Unigenes (including 902 singletons and 172 contigs), which were searched against the NCBI non-redundant (NR) protein database (E-value cutoff, e(-5)). Based on sequence similarity with known proteins, 579 differentially expressed genes in D. officinale were identified and classified into different functional categories by Gene Ontology (GO), Clusters of orthologous Groups of proteins (COGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The expression levels of 15 selected genes emblematic of symbiotic germination were confirmed via real-time quantitative PCR. These genes were classified into various categories, including defense and stress response, metabolism, transcriptional regulation, transport process and signal transduction pathways. All transcripts were upregulated in the symbiotically germinated seeds (SGS). The functions of these genes in symbiotic germination were predicted. Furthermore, two fungus-induced calcium-dependent protein kinases (CDPKs), which were upregulated 6.76- and 26.69-fold in SGS compared with un-germinated seeds (UGS), were cloned from D. officinale and characterized for the first time. This study provides the first global overview of genes putatively involved in D. officinale symbiotic seed germination and provides a foundation for further functional research regarding symbiotic relationships in orchids.

  20. ESTs analysis reveals putative genes involved in symbiotic seed germination in Dendrobium officinale.

    Directory of Open Access Journals (Sweden)

    Ming-Ming Zhao

    Full Text Available Dendrobiumofficinale (Orchidaceae is one of the world's most endangered plants with great medicinal value. In nature, D. officinale seeds must establish symbiotic relationships with fungi to germinate. However, the molecular events involved in the interaction between fungus and plant during this process are poorly understood. To isolate the genes involved in symbiotic germination, a suppression subtractive hybridization (SSH cDNA library of symbiotically germinated D. officinale seeds was constructed. From this library, 1437 expressed sequence tags (ESTs were clustered to 1074 Unigenes (including 902 singletons and 172 contigs, which were searched against the NCBI non-redundant (NR protein database (E-value cutoff, e(-5. Based on sequence similarity with known proteins, 579 differentially expressed genes in D. officinale were identified and classified into different functional categories by Gene Ontology (GO, Clusters of orthologous Groups of proteins (COGs and Kyoto Encyclopedia of Genes and Genomes (KEGG pathways. The expression levels of 15 selected genes emblematic of symbiotic germination were confirmed via real-time quantitative PCR. These genes were classified into various categories, including defense and stress response, metabolism, transcriptional regulation, transport process and signal transduction pathways. All transcripts were upregulated in the symbiotically germinated seeds (SGS. The functions of these genes in symbiotic germination were predicted. Furthermore, two fungus-induced calcium-dependent protein kinases (CDPKs, which were upregulated 6.76- and 26.69-fold in SGS compared with un-germinated seeds (UGS, were cloned from D. officinale and characterized for the first time. This study provides the first global overview of genes putatively involved in D. officinale symbiotic seed germination and provides a foundation for further functional research regarding symbiotic relationships in orchids.

  1. ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale

    Science.gov (United States)

    Zhao, Ming-Ming; Zhang, Gang; Zhang, Da-Wei; Hsiao, Yu-Yun; Guo, Shun-Xing

    2013-01-01

    Dendrobium officinale (Orchidaceae) is one of the world’s most endangered plants with great medicinal value. In nature, D . officinale seeds must establish symbiotic relationships with fungi to germinate. However, the molecular events involved in the interaction between fungus and plant during this process are poorly understood. To isolate the genes involved in symbiotic germination, a suppression subtractive hybridization (SSH) cDNA library of symbiotically germinated D . officinale seeds was constructed. From this library, 1437 expressed sequence tags (ESTs) were clustered to 1074 Unigenes (including 902 singletons and 172 contigs), which were searched against the NCBI non-redundant (NR) protein database (E-value cutoff, e-5). Based on sequence similarity with known proteins, 579 differentially expressed genes in D . officinale were identified and classified into different functional categories by Gene Ontology (GO), Clusters of orthologous Groups of proteins (COGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The expression levels of 15 selected genes emblematic of symbiotic germination were confirmed via real-time quantitative PCR. These genes were classified into various categories, including defense and stress response, metabolism, transcriptional regulation, transport process and signal transduction pathways. All transcripts were upregulated in the symbiotically germinated seeds (SGS). The functions of these genes in symbiotic germination were predicted. Furthermore, two fungus-induced calcium-dependent protein kinases (CDPKs), which were upregulated 6.76- and 26.69-fold in SGS compared with un-germinated seeds (UGS), were cloned from D . officinale and characterized for the first time. This study provides the first global overview of genes putatively involved in D . officinale symbiotic seed germination and provides a foundation for further functional research regarding symbiotic relationships in orchids. PMID:23967335

  2. Caracterização de águas residuárias de suinocultura provenientes de dois sistemas de tratamento

    Directory of Open Access Journals (Sweden)

    Tatiane Cristina Dal Bosco

    2015-12-01

    Full Text Available As águas residuárias de suinocultura apresentam características físico-químicas que variam de acordo com o sistema de higienização das baias, estádio de desenvolvimento dos animais, alimentação, condições climáticas, genética, entre outros fatores. No Brasil, o tratamento dessas águas residuárias, com elevada carga poluente, ocorre, geralmente, em esterqueiras e biodigestores. Na maioria das propriedades rurais, o destino final dos efluentes tratados é o solo. Pratica-se, portanto, o reúso de água na agricultura, que pode interferir na dinâmica de diversos poluentes no solo, como é o caso dos pesticidas. As águas residuárias apresentam-se na forma total e dissolvida, sendo esta a fração mais investigada atualmente na dinâmica desses poluentes. Neste sentido, objetivou-se utilizar a técnica de espectroscopia na região do infravermelho médio com transformada de Fourier na caracterização da matéria orgânica total e dissolvida proveniente de águas residuárias de suinocultura tratadas em biodigestor e esterqueira. Nos resultados, não foi possível observar diferenças na composição das águas residuárias pela espectroscopia infravermelha com transformada de Fourier. Entretanto, pode-se identificar a presença de materiais ainda pouco degradados e a ocorrência de grupos funcionais nitrogenados, que apontam para a liberação do nitrogênio no solo quando degradados, evidenciando o potencial de reúso dessas águas residuárias na agricultura como forma de fertirrigação. Palavras-chave: Infravermelho. Esterqueira. Biodigestor. Efluentes.

  3. Influência da salinidade da água no crescimento de seis genótipos de helicônias

    Directory of Open Access Journals (Sweden)

    Helder M. M. Barros

    2010-12-01

    Full Text Available No Nordeste brasileiro a qualidade das águas utilizadas em irrigação é muito variável tanto em termos geográficos como ao longo do ano, principalmente em pequenos açudes. Com a utilização de águas com altos níveis de salinidade aliada às condições climáticas, comumente encontradas no final do período de estiagem, há riscos de salinização dos solos irrigados com prejuízos, também, para as culturas. Para helicônias, não existem quaisquer indicativos relacionados ao efeito da salinidade da água no desenvolvimento. Propôs-se, neste trabalho, avaliar o crescimento na altura de planta mediante altura, número de folhas, teor de água na planta e fitomassa seca de seis genótipos de helicônias irrigados com águas salinas. O experimento foi conduzido em casa de vegetação pertencente à UFCG/Campina Grande, PB, compondo um esquema fatorial 6 x 6 em delineamento experimental de blocos ao acaso, com três repetições, cada um com dois vasos. O crescimento das helicônias foi afetado significativamente pelos níveis de salinidade. A H. latispatha cv. Red-Yellow Gyro e a H. psittacorum cv. Red Opal foram as helicônias menos afetadas pela salinidade. O teor de água na planta diminuiu com o aumento da CE da água de irrigação. Com o aumento da salinidade, ocorreu decréscimo na fitomassa seca. A H. x nickeriensis apresentou os menores índices em comparação com as demais helicônias, sendo mais sensível à salinidade.

  4. Fitotoxicidade e citogenotoxicidade da água e sedimento de córrego urbano em bioensaio com Lactuca sativa

    Directory of Open Access Journals (Sweden)

    Luiz C. de A. Rodrigues

    2013-10-01

    Full Text Available Objetivou-se, com este trabalho, avaliar a influência espacial e temporal da fitotoxicidade e da citogenotoxicidade da água e do sedimento de córrego urbano quanto às características germinativas e de crescimento inicial de Lactuca sativa. Amostras de água e de sedimento foram coletadas em 5 pontos do Córrego do Pântano (Alfenas, Minas Gerais, no período de outubro de 2010 a julho de 2011 e as concentrações dos metais Cd, Pb e Zn foram quantificadas. Os ensaios de fitotoxicidade e de citogenotoxicidade foram realizados com as amostras de água e extratos aquosos dos sedimentos. Os parâmetros avaliados foram taxa de germinação, comprimento de raízes, biomassa fresca e seca, índice mitótico e a frequência de anormalidades cromossômicas. Constataram-se maiores concentrações de Cd e Pb nas amostras de água coletadas nos meses com a ocorrência de precipitações pluviométricas. Água e sedimento apresentaram efeito fitotóxico sobre germinação, biomassa fresca e seca de Lactuca sativa. O comprimento de raízes foi estimulado e apenas as amostras de água reduziram o índice mitótico. Evidenciou-se, também, variação temporal significativa relacionada com o regime pluviométrico apenas para o teste de fitotoxicidade.

  5. Fatores determinantes das florações de cianobactérias na água do Rio Doce, Minas Gerais, Brasil

    Directory of Open Access Journals (Sweden)

    Fernando Antônio Jardim

    2014-09-01

    Full Text Available A partir de 2005 ocorreram sucessivas florações de cianobactérias tóxicas e não tóxicas na água do Rio Doce. Naquela época, foram identificadas as possíveis causas dessas florações e dentre elas se destacou principalmente o extenso período estival na região da bacia hidrográfica. O principal objetivo desse trabalho foi o de associar estatisticamente alguns parâmetros ambientais e de qualidade da água do rio em um período de cinco anos, para encontrar e explicar as possíveis correlações existentes. As cianobactérias estiveram presentes na água do Rio Doce nos 10 pontos de coleta avaliados pelo Instituto Mineiro de Gestão das Águas, no período de 2008 a 2012. Na análise de agrupamentos entre as estações de coleta, o ponto RD44 se destacou isoladamente e apresentou maiores densidades de termotolerantes durante o inverno. Por meio da análise de componentes principais, verificouse que as maiores densidades de cianobactérias estiveram associadas a reduções da temperatura da água, da turbidez, da concentração de fósforo total e condições características do período estival (inverno seco na água de rios em Minas Gerais. Nesse mesmo período, ocorreram elevações nas concentrações de clorofila a, oxigênio dissolvido, nitratos, da relação estequiométrica N/P e do Índice de Qualidade das Águas. Após 2009 as florações se tornaram sucessivas. Isso reforça a teoria de que o represamento da água do rio exerceu uma influência nas crescentes florações de cianobactérias, pois podem ter contribuído para a redução da turbidez da água do rio, para o aumento da concentração de nitratos e da densidade de termotolerantes.

  6. Uso de águas salinas como alternativa na irrigação e produção de forragem no semiárido nordestino

    Directory of Open Access Journals (Sweden)

    José L. de A. Silva

    Full Text Available RESUMO A produção de forragem no semiárido brasileiro é limitada, em virtude da variação da quantidade e qualidade da água, e ainda dos solos encontrados na região. Nesse contexto foi desenvolvido um experimento na Universidade Federal Rural do Semi-Árido - UFERSA, em Mossoró-RN. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 2 x 3, com três repetições. Os tratamentos resultaram da combinação de dois tipos de solos, um Argissolo Vermelho-Amarelo eutrófico (PVAe e um Cambissolo Háplico Ta eutrófico (CXve, com três níveis de salinidade da água de irrigação (A1 - 0,59; A2 - 2,75 e A3 - 5,00 dS m-1. As culturas utilizadas no experimento foram milho e sorgo. As variáveis analisadas ao final do experimento foram: área foliar, matéria seca e teor de proteína bruta. A resposta das culturas à salinidade é dependente das características físicas do solo, sendo mais tolerantes em solo com menor teor de argila. A mistura de águas salinas possibilita a produção satisfatória de forragem sem reduzir o teor protéico. A mistura de águas até se obter condutividade elétrica de 2,75 dS m-1 possibilita economia de 43,3% na água de boa qualidade, com perda de aproximadamente 22% na produção de biomassa.

  7. Transcriptome analysis reveals key differentially expressed genes involved in wheat grain development

    Directory of Open Access Journals (Sweden)

    Yonglong Yu

    2016-04-01

    Full Text Available Wheat seed development is an important physiological process of seed maturation and directly affects wheat yield and quality. In this study, we performed dynamic transcriptome microarray analysis of an elite Chinese bread wheat cultivar (Jimai 20 during grain development using the GeneChip Wheat Genome Array. Grain morphology and scanning electron microscope observations showed that the period of 11–15 days post-anthesis (DPA was a key stage for the synthesis and accumulation of seed starch. Genome-wide transcriptional profiling and significance analysis of microarrays revealed that the period from 11 to 15 DPA was more important than the 15–20 DPA stage for the synthesis and accumulation of nutritive reserves. Series test of cluster analysis of differential genes revealed five statistically significant gene expression profiles. Gene ontology annotation and enrichment analysis gave further information about differentially expressed genes, and MapMan analysis revealed expression changes within functional groups during seed development. Metabolic pathway network analysis showed that major and minor metabolic pathways regulate one another to ensure regular seed development and nutritive reserve accumulation. We performed gene co-expression network analysis to identify genes that play vital roles in seed development and identified several key genes involved in important metabolic pathways. The transcriptional expression of eight key genes involved in starch and protein synthesis and stress defense was further validated by qRT-PCR. Our results provide new insight into the molecular mechanisms of wheat seed development and the determinants of yield and quality.

  8. Systematic analysis of DEMETER-like DNA glycosylase genes shows lineage-specific Smi-miR7972 involved in SmDML1 regulation in Salvia miltiorrhiza.

    Science.gov (United States)

    Li, Jiang; Li, Caili; Lu, Shanfa

    2018-05-08

    DEMETER-like DNA glycosylases (DMLs) initiate the base excision repair-dependent DNA demethylation to regulate a wide range of biological processes in plants. Six putative SmDML genes, termed SmDML1-SmDML6, were identified from the genome of S. miltiorrhiza, an emerging model plant for Traditional Chinese Medicine (TCM) studies. Integrated analysis of gene structures, sequence features, conserved domains and motifs, phylogenetic analysis and differential expression showed the conservation and divergence of SmDMLs. SmDML1, SmDML2 and SmDML4 were significantly down-regulated by the treatment of 5Aza-dC, a general DNA methylation inhibitor, suggesting involvement of SmDMLs in genome DNA methylation change. SmDML1 was predicted and experimentally validated to be target of Smi-miR7972. Computational analysis of forty whole genome sequences and almost all of RNA-seq data from Lamiids revealed that MIR7972s were only distributed in some plants of the three orders, including Lamiales, Solanales and Boraginales, and the number of MIR7972 genes varied among species. It suggests that MIR7972 genes underwent expansion and loss during the evolution of some Lamiids species. Phylogenetic analysis of MIR7972s showed closer evolutionary relationships between MIR7972s in Boraginales and Solanales in comparison with Lamiales. These results provide a valuable resource for elucidating DNA demethylation mechanism in S. miltiorrhiza.

  9. QUALIDADE DA ÁGUA ARMAZENADA EM RESERVATÓRIOS DOMICILIARES: PARÂMETROS FÍSICO-QUÍMICOS E MICROBIOLÓGICOS

    OpenAIRE

    Juliana Alvares Duarte Bonini CAMPOS; Adalberto FARACHE FILHO; João Bosco FARIA

    2009-01-01

    Considerando-se que a água é um nutriente fundamental para manutenção da vida e reconhecendo-se a interferência dos diversos processos utilizados no tratamento da água na qualidade final do produto o presente estudo foi realizado visando verificar os efeitos dos reservatórios domiciliares nas características físico-químicas e microbiológicas da água utilizada para consumo humano na cidade de Araraquara (SP). Os resultados encontrados demonstraram discrepância nos teor...

  10. Crescimento e fitomassa da beterraba sob irrigação suplementar com água de diferentes concentrações salinas

    Directory of Open Access Journals (Sweden)

    Daniella Pereira dos Santos

    Full Text Available RESUMO O uso da água salina na agricultura é uma alternativa viável, tendo-se em vista o aumento da demanda de água doce. Objetivou-se, com este trabalho, avaliar o crescimento e a produção de fitomassa da beterraba, sob irrigação com água de diferentes concentrações salinas, em experimento em condição de campo, no Campus da Universidade Federal de Alagoas, em Arapiraca. Os tratamentos foram cinco níveis de condutividade elétrica (1,0; 2,0; 3,0; 4,0 e 5,0 dS m-1. O delineamento foi em blocos casualizados, com quatro repetições. O máximo rendimento da beterraba aos 27 dias após aplicação dos tratamentos salinos foi obtido com uma salinidade de 3,0 dS m-1, para as variáveis altura de planta (AP, diâmetro do caule (DC, comprimento da raiz (CR, fitomassa seca da parte aérea (FSPA e fitomassa seca total (FST. Aos 42 dias após aplicação dos tratamentos salinos, as variáveis fitomassa fresca da parte aérea (FFPA, fitomassa fresca da raiz (FFR, fitomassa fresca total (FFT, fitomassa seca da parte aérea (FSPA e fitomassa seca total (FST aumentaram com o aumento da salinidade da água. A chuva pode ter influenciado os resultados obtidos para as avaliações, realizadas aos 42 dias após aplicação dos tratamentos salinos.

  11. Viabilidade da irrigação do meloeiro com águas salinas em diferentes fases fenológicas Feasibility of irrigation of musk melon with salinity water in different phenological stages

    Directory of Open Access Journals (Sweden)

    Francisco de Queiroz Porto Filho

    2006-04-01

    Full Text Available Com o objetivo de estudar os efeitos da aplicação de águas de irrigação de diferentes salinidades no rendimento do melão irrigado por gotejamento e de associar a produção obtida com o custo da água utilizada, desenvolveu-se este trabalho em Mossoró-RN. Águas de diferentes salinidades (S1=0,6, S2=1,9, S3=3,2 e S4=4,5dS m-1, utilizadas de forma incremental em três estádios de desenvolvimento ou sem variar durante o ciclo da cultura, formaram dez tratamentos arranjados em blocos inteiramente casualizados com quatro repetições. O uso de águas salinas por longos períodos afetou a produção de melão. Substituições tardias na salinidade da água tenderam a não exercer efeito significativo sobre a produção do meloeiro. O tratamento irrigado com a água de menor salinidade durante todo ciclo apresentou, simultaneamente, o maior custo com água de irrigação e o maior lucro na produção de melão.This study was carried out in Mossoró, RN, Brazil, to evaluate the effects of different irrigation water salinity levels on yield of drip irrigated melon, and to relate yield with the cost of water. The waters of different salinities (S1=0.6, S2=1.9, S3=3.2 e S4=4.5dS m-1 were used both in incremental way in three different phenological stages and without replacement during the crop cycle totalizing ten treatments arranged in a completely randomized block design with four repetitions. The use of saline waters without substitutions affected melon production. The treatments irrigated with low salinity water presented simultaneously the higher cost of irrigation water and higher profits of melon cultivation.

  12. Identifying genes and gene networks involved in chromium metabolism and detoxification in Crambe abyssinica

    International Nuclear Information System (INIS)

    Zulfiqar, Asma; Paulose, Bibin; Chhikara, Sudesh; Dhankher, Om Parkash

    2011-01-01

    Chromium pollution is a serious environmental problem with few cost-effective remediation strategies available. Crambe abyssinica (a member of Brassicaseae), a non-food, fast growing high biomass crop, is an ideal candidate for phytoremediation of heavy metals contaminated soils. The present study used a PCR-Select Suppression Subtraction Hybridization approach in C. abyssinica to isolate differentially expressed genes in response to Cr exposure. A total of 72 differentially expressed subtracted cDNAs were sequenced and found to represent 43 genes. The subtracted cDNAs suggest that Cr stress significantly affects pathways related to stress/defense, ion transporters, sulfur assimilation, cell signaling, protein degradation, photosynthesis and cell metabolism. The regulation of these genes in response to Cr exposure was further confirmed by semi-quantitative RT-PCR. Characterization of these differentially expressed genes may enable the engineering of non-food, high-biomass plants, including C. abyssinica, for phytoremediation of Cr-contaminated soils and sediments. - Highlights: → Molecular mechanism of Cr uptake and detoxification in plants is not well known. → We identified differentially regulated genes upon Cr exposure in Crambe abyssinica. → 72 Cr-induced subtracted cDNAs were sequenced and found to represent 43 genes. → Pathways linked to stress, ion transport, and sulfur assimilation were affected. → This is the first Cr transcriptome study in a crop with phytoremediation potential. - This study describes the identification and isolation of differentially expressed genes involved in chromium metabolism and detoxification in a non-food industrial oil crop Crambe abyssinica.

  13. Identifying genes and gene networks involved in chromium metabolism and detoxification in Crambe abyssinica

    Energy Technology Data Exchange (ETDEWEB)

    Zulfiqar, Asma, E-mail: asmazulfiqar08@yahoo.com [Department of Plant, Soil, and Insect Sciences, 270 Stockbridge Road, University of Massachusetts Amherst, MA 01003 (United States); Paulose, Bibin, E-mail: bpaulose@psis.umass.edu [Department of Plant, Soil, and Insect Sciences, 270 Stockbridge Road, University of Massachusetts Amherst, MA 01003 (United States); Chhikara, Sudesh, E-mail: sudesh@psis.umass.edu [Department of Plant, Soil, and Insect Sciences, 270 Stockbridge Road, University of Massachusetts Amherst, MA 01003 (United States); Dhankher, Om Parkash, E-mail: parkash@psis.umass.edu [Department of Plant, Soil, and Insect Sciences, 270 Stockbridge Road, University of Massachusetts Amherst, MA 01003 (United States)

    2011-10-15

    Chromium pollution is a serious environmental problem with few cost-effective remediation strategies available. Crambe abyssinica (a member of Brassicaseae), a non-food, fast growing high biomass crop, is an ideal candidate for phytoremediation of heavy metals contaminated soils. The present study used a PCR-Select Suppression Subtraction Hybridization approach in C. abyssinica to isolate differentially expressed genes in response to Cr exposure. A total of 72 differentially expressed subtracted cDNAs were sequenced and found to represent 43 genes. The subtracted cDNAs suggest that Cr stress significantly affects pathways related to stress/defense, ion transporters, sulfur assimilation, cell signaling, protein degradation, photosynthesis and cell metabolism. The regulation of these genes in response to Cr exposure was further confirmed by semi-quantitative RT-PCR. Characterization of these differentially expressed genes may enable the engineering of non-food, high-biomass plants, including C. abyssinica, for phytoremediation of Cr-contaminated soils and sediments. - Highlights: > Molecular mechanism of Cr uptake and detoxification in plants is not well known. > We identified differentially regulated genes upon Cr exposure in Crambe abyssinica. > 72 Cr-induced subtracted cDNAs were sequenced and found to represent 43 genes. > Pathways linked to stress, ion transport, and sulfur assimilation were affected. > This is the first Cr transcriptome study in a crop with phytoremediation potential. - This study describes the identification and isolation of differentially expressed genes involved in chromium metabolism and detoxification in a non-food industrial oil crop Crambe abyssinica.

  14. Manejo do solo, água e nitrogênio no cultivo de feijão

    Directory of Open Access Journals (Sweden)

    Arf Orivaldo

    2004-01-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito do preparo e não preparo do solo, de doses de N aplicadas em cobertura e da irrigação na produtividade do feijoeiro cv. IAC Carioca Eté. O delineamento experimental foi o de blocos casualizados em esquema fatorial com quatro repetições. Os tratamentos constituíram-se da combinação de três lâminas de água, com preparo do solo com arado de aiveca, com grade pesada e sem preparo (plantio direto e com 0, 30, 60, 90 e 120 kg ha-1 de N aplicados em cobertura aos 15 dias após a emergência das plantas. A irrigação foi realizada com um sistema fixo de irrigação convencional por aspersão e no manejo de água foram utilizados diferentes coeficientes de cultura (Kc distribuídos em períodos compreendidos entre a emergência e a colheita. A menor lâmina de água propiciou produtividade de grãos semelhante à das outras lâminas, mas com menor custo. O preparo do solo com arado de aiveca não diferenciou do preparo com grade aradora e proporcionou maior produtividade de grãos em relação ao plantio direto. A adubação nitrogenada não afetou a produtividade de grãos.

  15. Modelo para estimativa da infiltração de água e perfil de umidade do solo

    Directory of Open Access Journals (Sweden)

    Roberto Avelino Cecílio

    2013-04-01

    Full Text Available A adequada modelagem da infiltração de água no solo é fundamental para estimação do movimento de água, erosão hídrica, recarga e contaminação de aquíferos. Este trabalho apresenta um modelo para estimativa da infiltração de água no solo (GAML-c, com base no modelo de Green-Ampt-Mein-Larson, que provê descrição da geometria e do deslocamento da frente de umedecimento no solo. Testes experimentais foram conduzidos em Latossolo Vermelho-Amarelo para avaliar o GAML-c, usando-se quatro diferentes cenários: considerando a condutividade hidráulica do solo igual à taxa de infiltração estável (Tie e a umidade máxima do solo igual ao teor de água na zona de transmissão (θw (TW; condutividade hidráulica do solo igual à do solo saturado (K0 e a umidade máxima do solo igual θw (KW; condutividade hidráulica do solo igual à Tie e a umidade máxima do solo igual ao teor de água na saturação (θs (TS; e condutividade hidráulica do solo igual a K0 e a umidade máxima do solo igual θs (KS. Verificou-se que o GAML-c no cenário TW foi o melhor estimador do perfil de umidade do solo, resultando em aceitáveis estimativas da infiltração de água.

  16. Bactérias na água de abastecimento da cidade de Piracicaba

    OpenAIRE

    Marina Gumiere Alves

    2008-01-01

    Espera-se que a água esteja dentro de um padrão de qualidade após passar pelos processos de tratamento, que deve ser mantido durante a distribuição chegando aos pontos de consumo em condições de jamais prejudicar a saúde humana e animal. Contudo, há vários problemas de saúde que mundialmente vêm sendo associados ao suprimento público de água, fato que tem exigido um controle de qualidade cada vez mais rigoroso. Apesar dos recursos analíticos disponíveis, que facilitam a detecção e quantificaç...

  17. Resíduo agroindustrial na formação de mudas ornamentais irrigadas com água residuária

    Directory of Open Access Journals (Sweden)

    Aline Costa Ferreira

    2014-06-01

    Full Text Available O reuso de água de esgoto tratada juntamente com o uso de resíduos industriais para a formação de mudas de girassol ornamental vem sendo uma alternativa econômica para o estado da Paraíba. Nesse contexto, a pesquisa foi realizada objetivando-se a utilização de resíduo agroindustrial na formação de mudas ornamentais irrigadas com água residuária. Desenvolvido em ambiente protegido na Universidade Federal de Campina Grande – UFCG, localizado no Estado da Paraíba-PB, utilizaram-se os seguintes tratamentos: 100% substrato comercial (SC, 100% solo (S, 100% fibra de coco (FC e 50% (SC misturado a 50% (FC, combinados com 2 tipos de água (A1 – abastecimento; A2 – residuária. O delineamento experimental foi em blocos ao acaso, num esquema fatorial de 4 x 2, com 3 repetições e 3 plantas por repetição. Foram avaliados a altura de planta (AP, o número de folhas (NF e o diâmetro do caule (DC, comprimento radicular (CR, fitomassa fresca da parte aérea (FFPA e da raiz (FFR, fitomassa seca da parte aérea (FSPA e da raiz (FSR.  As maiores médias foram relacionadas à utilização de água residuária e resultados obtidos pelo uso dos diversos substratos, os quais tiveram significância para algumas variáveis, em pelo menos uma época de avaliação. Percebe-se a importância do uso de água residuária como fertirrigação das mudas e o reaproveitamento de resíduos industriais como substrato, sendo uma alternativa com grande potencial e viável para os pequenos agricultores.

  18. Characterization of the ptr5{sup +} gene involved in nuclear mRNA export in fission yeast

    Energy Technology Data Exchange (ETDEWEB)

    Watanabe, Nobuyoshi; Ikeda, Terumasa; Mizuki, Fumitaka [Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto 860-8555 (Japan); Tani, Tokio, E-mail: ttani@sci.kumamoto-u.ac.jp [Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kurokami, Kumamoto 860-8555 (Japan)

    2012-02-03

    Highlights: Black-Right-Pointing-Pointer We cloned the ptr5{sup +} gene involved in nuclear mRNA export in fission yeast. Black-Right-Pointing-Pointer The ptr5{sup +} gene was found to encode nucleoporin 85 (Nup85). Black-Right-Pointing-Pointer Seh1p and Mlo3p are multi-copy suppressors for the ptr5 mutation. Black-Right-Pointing-Pointer Ptr5p/Nup85p functions in nuclear mRNA export through the mRNA export factor Rae1p. Black-Right-Pointing-Pointer Ptr5p/Nup85p interacts genetically with pre-mRNA splicing factors. -- Abstract: To analyze the mechanisms of mRNA export from the nucleus to the cytoplasm, we have isolated eleven mutants, ptr [poly(A){sup +} RNA transport] 1 to 11, which accumulate poly(A){sup +} RNA in the nucleus at a nonpermissive temperature in Schizosaccharomyces pombe. Of those, the ptr5-1 mutant shows dots- or a ring-like accumulation of poly(A){sup +} RNA at the nuclear periphery after shifting to the nonpermissive temperature. We cloned the ptr5{sup +} gene and found that it encodes a component of the nuclear pore complex (NPC), nucleoporin 85 (Nup85). The ptr5-1 mutant shows no defects in protein transport, suggesting the specific involvement of Ptr5p/Nup85p in nuclear mRNA export in S. pombe. We identified Seh1p, a nucleoporin interacting with Nup85p, an mRNA-binding protein Mlo3p, and Sac3p, a component of the TREX-2 complex involved in coupling of nuclear mRNA export with transcription, as multi-copy suppressors for the ptr5-1 mutation. In addition, we found that the ptr5-1 mutation is synthetically lethal with a mutation of the mRNA export factor Rae1p, and that the double mutant exaggerates defective nuclear mRNA export, suggesting that Ptr5p/Nup85p is involved in nuclear mRNA export through Rae1p. Interestingly, the ptr5-1 mutation also showed synthetic effects with several prp pre-mRNA splicing mutations, suggesting a functional linkage between the NPCs and the splicing apparatus in the yeast nucleus.

  19. Identification of gene expression patterns crucially involved in experimental autoimmune encephalomyelitis and multiple sclerosis

    Directory of Open Access Journals (Sweden)

    Martin M. Herrmann

    2016-10-01

    Full Text Available After encounter with a central nervous system (CNS-derived autoantigen, lymphocytes leave the lymph nodes and enter the CNS. This event leads only rarely to subsequent tissue damage. Genes relevant to CNS pathology after cell infiltration are largely undefined. Myelin-oligodendrocyte-glycoprotein (MOG-induced experimental autoimmune encephalomyelitis (EAE is an animal model of multiple sclerosis (MS, a chronic autoimmune disease of the CNS that results in disability. To assess genes that are involved in encephalitogenicity and subsequent tissue damage mediated by CNS-infiltrating cells, we performed a DNA microarray analysis from cells derived from lymph nodes and eluted from CNS in LEW.1AV1 (RT1av1 rats immunized with MOG 91-108. The data was compared to immunizations with adjuvant alone or naive rats and to immunizations with the immunogenic but not encephalitogenic MOG 73-90 peptide. Here, we show involvement of Cd38, Cxcr4 and Akt and confirm these findings by the use of Cd38-knockout (B6.129P2-Cd38tm1Lnd/J mice, S1P-receptor modulation during EAE and quantitative expression analysis in individuals with MS. The hereby-defined underlying pathways indicate cellular activation and migration pathways mediated by G-protein-coupled receptors as crucial events in CNS tissue damage. These pathways can be further explored for novel therapeutic interventions.

  20. Estudo sobre Reabilitação Interior de Reservatórios para Água Potável

    OpenAIRE

    Guedes, Daniela Sofia Gomes Tavares

    2014-01-01

    No presente relatório é apresentado um estudo, realizado na forma de estágio curricular, na empresa Águas do Douro e Paiva, S.A., doravante AdDP, entre 31 de Janeiro e 31 de Julho de 2014, sobre reabilitação interior de reservatórios para água potável. Inicialmente é feito um enquadramento ao tema, com uma abordagem às características genéricas dos reservatórios de água potável e às principais patologias que se verificam no interior desses reservatórios. De seguida, são detalhadas as prin...

  1. The ipdC, hisC1 and hisC2 genes involved in indole-3-acetic production used as alternative phylogenetic markers in Azospirillum brasilense.

    Science.gov (United States)

    Jijón-Moreno, Saúl; Marcos-Jiménez, Cynthia; Pedraza, Raúl O; Ramírez-Mata, Alberto; de Salamone, I García; Fernández-Scavino, Ana; Vásquez-Hernández, Claudia A; Soto-Urzúa, Lucia; Baca, Beatriz E

    2015-06-01

    Plant growth-promoting bacteria of the genus Azospirillum are present in the rhizosphere and as endophytes of many crops. In this research we studied 40 Azospirillum strains isolated from different plants and geographic regions. They were first characterized by 16S rDNA restriction analysis, and their phylogenetic position was established by sequencing the genes 16S rDNA, ipdC, hisC1, and hisC2. The latter three genes are involved in the indole-3-pyruvic acid (IPyA) biosynthesis pathway of indole-3-acetic acid (IAA). Furthermore, the suitability of the 16S-23S rDNA intergenic spacer sequence (IGS) for the differentiation of closely related Azospirillum taxa and development of PCR protocols allows for specific detection of strains. The IGS-RFLP analysis enabled intraspecies differentiation, particularly of Azospirillum brasilense and Azospirillum lipoferum strains. Results demonstrated that the ipdC, hisC1, and hisC2 genes are highly conserved in all the assessed A. brasilense isolates, suggesting that these genes can be used as an alternative phylogenetic marker. In addition, IAA production determined by HPLC ranged from 0.17 to 98.2 μg mg(-1) protein. Southern hybridization with the A. brasilense ipdC gene probe did not show, a hybridization signal with A. lipoferum, Azospirillum amazonense, Azospirillum halopreferans and Azospirillum irakense genomic DNA. This suggests that these species produce IAA by other pathways. Because IAA is mainly synthesized via the IPyA pathway in A. brasilense strains, a species that is used worldwide in agriculture, the identification of ipdC, hisC1, and hisC2 genes by PCR may be suitable for selecting exploitable strains.

  2. Uso de água residual do ar condicionado e de agua pluvial como gestão da oferta em uma edificação comercial: estudo de caso

    Directory of Open Access Journals (Sweden)

    Celso Silva Bastos

    2013-12-01

    Full Text Available O conceito do uso racional para a conservação de água consiste na gestão da demanda e da oferta de água, com ações voltadas para o fornecimento de fontes alternativas visando suprir o uso em áreas menos nobres por águas de qualidade inferior. Este trabalho tem por objetivo mostrar a aplicação de um sistema que aproveita a água drenada dos evaporadores de ar-condicionado e das águas pluviais como fontes alternativas. Verificou-se que cada evaporador produz 4,8 litros/água/hora, considerando 10 horas de uso diário, o volume acumulado chegaria a 48 litros/dia por aparelho que multiplicado pelo total de 137 unidades chega-se ao volume diário de 4.298 litros o que representa 77,72% da demanda das descargas sanitárias que é de 5.530 litros/dia. Em relação ao aproveitamento de águas pluviais, a previsão de acumulação refere-se a 10% da área do terreno que multiplicado pelo índice de 0,05 e pela retenção de 1 hora, obtém o volume de 14,8 m3 . Pretende-se com essa prática, alcançar uma redução significativa do volume de água potável, hoje utilizada praticamente para a realização de todas as atividades, contribuindo assim para uma obra mais sustentável, minimizando os impactos ambientais causados pela construção.

  3. Colistin-Resistant, Lipopolysaccharide-Deficient Acinetobacter baumannii Responds to Lipopolysaccharide Loss through Increased Expression of Genes Involved in the Synthesis and Transport of Lipoproteins, Phospholipids, and Poly-β-1,6-N-Acetylglucosamine

    Science.gov (United States)

    Henry, Rebekah; Vithanage, Nuwan; Harrison, Paul; Seemann, Torsten; Coutts, Scott; Moffatt, Jennifer H.; Nation, Roger L.; Li, Jian; Harper, Marina; Adler, Ben

    2012-01-01

    We recently demonstrated that colistin resistance in Acinetobacter baumannii can result from mutational inactivation of genes essential for lipid A biosynthesis (Moffatt JH, et al., Antimicrob. Agents Chemother. 54:4971–4977). Consequently, strains harboring these mutations are unable to produce the major Gram-negative bacterial surface component, lipopolysaccharide (LPS). To understand how A. baumannii compensates for the lack of LPS, we compared the transcriptional profile of the A. baumannii type strain ATCC 19606 to that of an isogenic, LPS-deficient, lpxA mutant strain. The analysis of the expression profiles indicated that the LPS-deficient strain showed increased expression of many genes involved in cell envelope and membrane biogenesis. In particular, upregulated genes included those involved in the Lol lipoprotein transport system and the Mla-retrograde phospholipid transport system. In addition, genes involved in the synthesis and transport of poly-β-1,6-N-acetylglucosamine (PNAG) also were upregulated, and a corresponding increase in PNAG production was observed. The LPS-deficient strain also exhibited the reduced expression of genes predicted to encode the fimbrial subunit FimA and a type VI secretion system (T6SS). The reduced expression of genes involved in T6SS correlated with the detection of the T6SS-effector protein AssC in culture supernatants of the A. baumannii wild-type strain but not in the LPS-deficient strain. Taken together, these data show that, in response to total LPS loss, A. baumannii alters the expression of critical transport and biosynthesis systems associated with modulating the composition and structure of the bacterial surface. PMID:22024825

  4. Colistin-resistant, lipopolysaccharide-deficient Acinetobacter baumannii responds to lipopolysaccharide loss through increased expression of genes involved in the synthesis and transport of lipoproteins, phospholipids, and poly-β-1,6-N-acetylglucosamine.

    Science.gov (United States)

    Henry, Rebekah; Vithanage, Nuwan; Harrison, Paul; Seemann, Torsten; Coutts, Scott; Moffatt, Jennifer H; Nation, Roger L; Li, Jian; Harper, Marina; Adler, Ben; Boyce, John D

    2012-01-01

    We recently demonstrated that colistin resistance in Acinetobacter baumannii can result from mutational inactivation of genes essential for lipid A biosynthesis (Moffatt JH, et al., Antimicrob. Agents Chemother. 54:4971-4977). Consequently, strains harboring these mutations are unable to produce the major Gram-negative bacterial surface component, lipopolysaccharide (LPS). To understand how A. baumannii compensates for the lack of LPS, we compared the transcriptional profile of the A. baumannii type strain ATCC 19606 to that of an isogenic, LPS-deficient, lpxA mutant strain. The analysis of the expression profiles indicated that the LPS-deficient strain showed increased expression of many genes involved in cell envelope and membrane biogenesis. In particular, upregulated genes included those involved in the Lol lipoprotein transport system and the Mla-retrograde phospholipid transport system. In addition, genes involved in the synthesis and transport of poly-β-1,6-N-acetylglucosamine (PNAG) also were upregulated, and a corresponding increase in PNAG production was observed. The LPS-deficient strain also exhibited the reduced expression of genes predicted to encode the fimbrial subunit FimA and a type VI secretion system (T6SS). The reduced expression of genes involved in T6SS correlated with the detection of the T6SS-effector protein AssC in culture supernatants of the A. baumannii wild-type strain but not in the LPS-deficient strain. Taken together, these data show that, in response to total LPS loss, A. baumannii alters the expression of critical transport and biosynthesis systems associated with modulating the composition and structure of the bacterial surface.

  5. Fungos filamentosos na água e formando biofilmes na rede de distribuição de água potável do Sistema Alto do Céu, Recife-PE/

    OpenAIRE

    OLIVEIRA, Helena Maria Bezerra de

    2010-01-01

    As várias questões em potencial associadas aos fungos em água de consumo incluem obstruções da canalização, alterações como odor, sabor, pigmentos, formação de biofilmes, disseminação de fungos patogênicos e produção de micotoxinas. As bactérias e os fungos têm sido bem sucedidos nesse meio por possuírem estratégias metabólicas que lhes permitem sobreviver em ambientes oligotróficos como água potável. Assim, algumas espécies de fungos podem ser encontradas habitando sistemas de distribuição d...

  6. Polymorphisms in genes involved in the estrogen pathway and mammographic density

    Directory of Open Access Journals (Sweden)

    Dumas Isabelle

    2010-11-01

    Full Text Available Abstract Background Single nucleotide polymorphisms (SNPs in genes involved in the estrogen pathway appear to be associated with breast cancer risk and possibly with mammographic density (MD, but little is known of these associations among premenopausal women. This study examines the association of 11 polymorphisms in five estrogen-related genes (estrogen receptors alpha and beta (ERα, ERβ, 17β-hydroxysteroid dehydrogenase 1 (HSD17B1, catechol-O-methyltransferase (COMT, cytochrome P450 1B1 (CYP1B1 with premenopausal MD. Effect modification of four estrogen-related factors (parity, age at menarche, hormonal derivatives use and body mass index (BMI on this relation is also assessed. Methods Polymorphisms were genotyped in 741 premenopausal Caucasian women whose MD was measured in absolute density (AD, cm2 and percent density using a computer-assisted method. Multivariate linear models were used to examine the associations (Ptrend and interactions (Pi. Results None of the SNPs showed a statistically significant association with AD. However, each additional rare allele of rs1056836 CYP1B1 was associated with a reduction in AD among nulliparous women (Ptrend = 0.004, while no association was observed among parous women (Ptrend = 0.62; Pi = 0.02. An increase in the number of rare alleles of the HSD17B1 SNP (rs598126 and rs2010750 was associated with an increase in AD among women who never used hormonal derivatives (Ptrend = 0.06 and Ptrend = 0.04, respectively, but with a decrease in AD among past hormonal derivatives users (Ptrend = 0.04; Pi = 0.02 and Ptrend = 0.08; Pi = 0.01, respectively. Moreover, a negative association of rs598126 HSD17B1 SNP with AD was observed among women with higher BMI (>median (Ptrend = 0.01; Pi = 0.02. A negative association between an increased number of rare alleles of COMT rs4680 SNP and AD was limited to women who never used hormonal derivatives (Ptrend = 0.02; Pi = 0.03 or with late age at menarche (>median

  7. Polymorphisms in genes involved in the estrogen pathway and mammographic density

    International Nuclear Information System (INIS)

    Dumas, Isabelle; Diorio, Caroline

    2010-01-01

    Single nucleotide polymorphisms (SNPs) in genes involved in the estrogen pathway appear to be associated with breast cancer risk and possibly with mammographic density (MD), but little is known of these associations among premenopausal women. This study examines the association of 11 polymorphisms in five estrogen-related genes (estrogen receptors alpha and beta (ERα, ERβ), 17β-hydroxysteroid dehydrogenase 1 (HSD17B1), catechol-O-methyltransferase (COMT), cytochrome P450 1B1 (CYP1B1)) with premenopausal MD. Effect modification of four estrogen-related factors (parity, age at menarche, hormonal derivatives use and body mass index (BMI)) on this relation is also assessed. Polymorphisms were genotyped in 741 premenopausal Caucasian women whose MD was measured in absolute density (AD, cm 2 ) and percent density using a computer-assisted method. Multivariate linear models were used to examine the associations (P trend ) and interactions (P i ). None of the SNPs showed a statistically significant association with AD. However, each additional rare allele of rs1056836 CYP1B1 was associated with a reduction in AD among nulliparous women (P trend = 0.004), while no association was observed among parous women (P trend = 0.62; P i = 0.02). An increase in the number of rare alleles of the HSD17B1 SNP (rs598126 and rs2010750) was associated with an increase in AD among women who never used hormonal derivatives (P trend = 0.06 and P trend = 0.04, respectively), but with a decrease in AD among past hormonal derivatives users (P trend = 0.04; P i = 0.02 and P trend = 0.08; P i = 0.01, respectively). Moreover, a negative association of rs598126 HSD17B1 SNP with AD was observed among women with higher BMI (>median) (P trend = 0.01; P i = 0.02). A negative association between an increased number of rare alleles of COMT rs4680 SNP and AD was limited to women who never used hormonal derivatives (P trend = 0.02; P i = 0.03) or with late age at menarche (>median) (P trend = 0.03; P i

  8. Investigation of associations between NR1D1, RORA and RORB genes and bipolar disorder.

    Directory of Open Access Journals (Sweden)

    Yin-Chieh Lai

    Full Text Available Several genes that are involved in the regulation of circadian rhythms are implicated in the susceptibility to bipolar disorder (BD. The current study aimed to investigate the relationships between genetic variants in NR1D1 RORA, and RORB genes and BD in the Han Chinese population. We conducted a case-control genetic association study with two samples of BD patients and healthy controls. Sample I consisted of 280 BD patients and 200 controls. Sample II consisted of 448 BD patients and 1770 healthy controls. 27 single nucleotide polymorphisms in the NR1D1, RORA, and RORB genes were genotyped using GoldenGate VeraCode assays in sample I, and 492 markers in the three genes were genotyped using Affymetrix Genome-Wide CHB Array in sample II. Single marker and gene-based association analyses were performed using PLINK. A combined p-value for the joining effects of all markers within a gene was calculated using the rank truncated product method. Multifactor dimensionality reduction (MDR method was also applied to test gene-gene interactions in sample I. All markers were in Hardy-Weinberg equilibrium (P>0.001. In sample I, the associations with BD were observed for rs4774388 in RORA (OR = 1.53, empirical p-value, P = 0.024, and rs1327836 in RORB (OR = 1.75, P = 0.003. In Sample II, there were 45 SNPs showed associations with BD, and the most significant marker in RORA was rs11639084 (OR = 0.69, P = 0.002, and in RORB was rs17611535 (OR = 3.15, P = 0.027. A combined p-value of 1.6×10-6, 0.7, and 1.0 was obtained for RORA, RORB and NR1D1, respectively, indicting a strong association for RORA with the risk of developing BD. A four way interaction was found among markers in NR1D1, RORA, and RORB with the testing accuracy 53.25% and a cross-validation consistency of 8 out of 10. In sample II, 45 markers had empirical p-values less than 0.05. The most significant markers in RORA and RORB genes were rs11639084 (OR = 0.69, P = 0.002, and rs17611535 (OR = 3

  9. Isoeugenol monooxygenase and its putative regulatory gene are located in the eugenol metabolic gene cluster in Pseudomonas nitroreducens Jin1.

    Science.gov (United States)

    Ryu, Ji-Young; Seo, Jiyoung; Unno, Tatsuya; Ahn, Joong-Hoon; Yan, Tao; Sadowsky, Michael J; Hur, Hor-Gil

    2010-03-01

    The plant-derived phenylpropanoids eugenol and isoeugenol have been proposed as useful precursors for the production of natural vanillin. Genes involved in the metabolism of eugenol and isoeugenol were clustered in region of about a 30 kb of Pseudomonas nitroreducens Jin1. Two of the 23 ORFs in this region, ORFs 26 (iemR) and 27 (iem), were predicted to be involved in the conversion of isoeugenol to vanillin. The deduced amino acid sequence of isoeugenol monooxygenase (Iem) of strain Jin1 had 81.4% identity to isoeugenol monooxygenase from Pseudomonas putida IE27, which also transforms isoeugenol to vanillin. Iem was expressed in E. coli BL21(DE3) and was found to lead to isoeugenol to vanillin transformation. Deletion and cloning analyses indicated that the gene iemR, located upstream of iem, is required for expression of iem in the presence of isoeugenol, suggesting it to be the iem regulatory gene. Reverse transcription, real-time PCR analyses indicated that the genes involved in the metabolism of eugenol and isoeugenol were differently induced by isoeugenol, eugenol, and vanillin.

  10. Identification of new developmentally regulated genes involved in Streptomyces coelicolor sporulation.

    Science.gov (United States)

    Salerno, Paola; Persson, Jessica; Bucca, Giselda; Laing, Emma; Ausmees, Nora; Smith, Colin P; Flärdh, Klas

    2013-12-05

    The sporulation of aerial hyphae of Streptomyces coelicolor is a complex developmental process. Only a limited number of the genes involved in this intriguing morphological differentiation programme are known, including some key regulatory genes. The aim of this study was to expand our knowledge of the gene repertoire involved in S. coelicolor sporulation. We report a DNA microarray-based investigation of developmentally controlled gene expression in S. coelicolor. By comparing global transcription patterns of the wild-type parent and two mutants lacking key regulators of aerial hyphal sporulation, we found a total of 114 genes that had significantly different expression in at least one of the two mutants compared to the wild-type during sporulation. A whiA mutant showed the largest effects on gene expression, while only a few genes were specifically affected by whiH mutation. Seven new sporulation loci were investigated in more detail with respect to expression patterns and mutant phenotypes. These included SCO7449-7451 that affect spore pigment biogenesis; SCO1773-1774 that encode an L-alanine dehydrogenase and a regulator-like protein and are required for maturation of spores; SCO3857 that encodes a protein highly similar to a nosiheptide resistance regulator and affects spore maturation; and four additional loci (SCO4421, SCO4157, SCO0934, SCO1195) that show developmental regulation but no overt mutant phenotype. Furthermore, we describe a new promoter-probe vector that takes advantage of the red fluorescent protein mCherry as a reporter of cell type-specific promoter activity. Aerial hyphal sporulation in S. coelicolor is a technically challenging process for global transcriptomic investigations since it occurs only as a small fraction of the colony biomass and is not highly synchronized. Here we show that by comparing a wild-type to mutants lacking regulators that are specifically affecting processes in aerial hypha, it is possible to identify previously

  11. Integração de métodos para o uso eficiente de água em ambientes urbanos

    OpenAIRE

    Silva, Marcos Dornelas Freitas Machado e

    2014-01-01

    Os problemas relacionados à escassez hídrica estão em evidência e a busca por tecnologias para redução do consumo de água e por fontes alternativas de abastecimento é incentivada. Varias ações têm sido exploradas como a melhoria do monitoramento do consumo, identificação de vazamentos, instalação de equipamentos economizadores e uso de fontes alternativas, como água cinza e água pluvial. Esta última é o foco de vários estudos, devido aos inúmeros serviços sociais, ambientais e econômicos que ...

  12. Comparison of gene expression profiles of HepG2 cells exposed to Crambescins C1 and A1

    Directory of Open Access Journals (Sweden)

    María R. Sánchez

    2014-06-01

    Full Text Available Crambescins are guanidine alkaloids firstly isolated in the early 90s from the encrusting Mediterranean sponge Crambe crambe (Schmidt, 1862 (Bondu et al., 2012, Laville et al., 2009, Berlinck et al., 1990. C. crambe derivatives are divided in two families named crambescins and crambescidins (Gerlinck et al., 1992. Although data on the bioactivity of these compounds is scarce, crambescidins have recognized cytotoxic, antifungal, antioxidant, antimicrobial and antiviral activities (Buscema and Van de Vyver, 1985, Jares-Erijman., 1998, Olszewski et al., 2004, Lazaro et al., 2006, Suna et al., 2007, AOKI et al., 2004. Recently we have carefully evaluated the cytotoxic activity of C816 over several human tumor cell types and characterized some of the cellular mechanisms responsible of the anti-proliferative effect of this compound on human liver-derived tumor cells (Rubiolo et al., 2013. Taking this into account, and to better understand the mechanism of action of crambescins and their potential as therapeutic agents, we made a comparative gene expression profiling of HepG2 cells after crambescin C1 (C1 and crambescin A1 (CA1 exposures. Results have shown that C1 induces genes involved in sterol and glucose metabolisms and metabolism involving growth factors. It also down regulates genes mainly involved in cell cycle control, DNA replication, recombination and repair, and drug metabolism. Flow cytometry assays revealed that C1 produces a G0/G1 arrest in HepG2 cell cycle progression. CA1 also down-regulates genes involved in cell cycle regulation, DNA recombination and pathways related to tumor cells proliferation with lower potency when compared to C1.

  13. The hnRNP 2H9 gene, which is involved in the splicing reaction, is a multiply spliced gene

    DEFF Research Database (Denmark)

    Honoré, B

    2000-01-01

    The hnRNP 2H9 gene products are involved in the splicing process and participate in early heat shock-induced splicing arrest. By combining low/high stringency hybridisation, database search, Northern and Western blotting it is shown that the gene is alternatively spliced into at least six...

  14. Eficiência do uso da água na produção do pinhão-manso no semiárido nordestino

    Directory of Open Access Journals (Sweden)

    Clayton Moura de Carvalho

    2015-06-01

    Full Text Available O governo do estado do Ceará tem buscado, sem muito sucesso, revitalizar a cultura do algodão, entretanto, os seus elevados custos fitossanitários têm impedido a permanência da área cultivada. Um das alternativas viáveis para a ocupação de espaços provenientes da ausência do algodão é o cultivo do pinhão manso visando à produção do biodiesel. Face ao exposto, este trabalho objetivou avaliar os efeitos de diferentes níveis de lâminas de água e adubação nitrogenada no uso eficiente da água na produção de frutos, sementes e albúmen do pinhão manso. O trabalho foi desenvolvido na área experimental da Fazenda Bandeira pertencente à Agroempresa Brasil Ecodiesel, situada em Crateús – CE, localizada em 05˚23’25’’S, 40˚57’38’’W e 717 m de altitude. O delineamento experimental adotado foi o de blocos ao acaso no esquema de parcelas subdivididas. Os tratamentos foram compostos a partir da combinação de cinco lâminas de água (50%, 75%, 100%, 125% e 150% da evaporação do Tanque Classe “A”, e quatro níveis de adubação nitrogenada (N1 = 0 kg ha-1 de N; N2 = 25 kg ha-1 de N; N3 = 50 kg ha-1 de N; N4 = 75 kg ha-1 de N, com três repetições. As variáveis analisadas foram a eficiência do uso da água na produção de frutos, sementes e albúmen. Os maiores valores da eficiência do uso da água na produção de frutos, sementes e albúmen foram encontrados com a menor lâmina aplicada. Como o semiárido cearense apresenta naturalmente condições de escassez hídrica torna-se viável o cultivo do pinhão manso com a lâmina de 735 mm, representando uma economia de água de 124% em relação à maior lâmina aplicada. No que se refere às doses de adubação, as dosagens utilizadas neste experimento não influenciaram nos resultados das variáveis estudadas.

  15. Análise De Oportunidades E Desafios Para O Nexo Água-energia

    OpenAIRE

    Mariani; L; Guarenghi; MM; Mito; JYL; Cavaliero; CKN; Galvao; RRD

    2016-01-01

    O desenvolvimento econômico, o crescimento da população mundial e o aumento da urbanização, tendem a aumentar a demanda mundial por água e energia, intensificando os conflitos entre os setores hídrico e energético e gerando impactos sobre o meio ambiente. Além da interferência direta dos seres humanos sobre esses recursos, as mudanças climáticas e eventos climáticos extremos têm afetado a disponibilidade de água e, consequentemente, a geração de energia de forma significativa. Este trabalho t...

  16. Sistema detector de vazamentos em instalações prediais de água fria

    OpenAIRE

    Araújo, Ícaro Bezerra Queiroz de

    2015-01-01

    Instalações prediais de água fria são partes fundamentais em qualquer modelo de habitação, sejam residências ou condomínios. Porém, tais sistemas estão sujeitos a falhas, que podem variar desde um vazamento em um aparelho, até falhas na estrutura dos reservatórios de água e sistema de distribuição. Estas falhas são responsáveis por altos custos econômicos e ambientais. Visando reduzir estas perdas, este trabalho propõe o desenvolvimento de um sistema capaz de detectar a presenç...

  17. Assessment of "2"2"6Ra, "2"2"8Ra and "2"1"0Pb concentration in mineral waters from Parque das Águas de Lambari and Contendas, MG and evaluation of the committed effective doses

    International Nuclear Information System (INIS)

    Wakasugi, Denise S.M.; Damatto, Sandra R.

    2017-01-01

    The exploration of several Brazilian hydromineral resorts, that have springs of radioactive mineral water consumed by the population support the relevance of the present work. This study is being developed in the IPEN with the aid of the responsible for Parque das Águas de Lambari and Contendas. Thereby, this work aims to determine the activity concentrations of "2"2"6Ra, "2"2"8Ra and "2"1"0Pb to verify the influence of seasonality in these water parks springs. Thereunto, gross alpha and beta measurements were used after radiochemical separation for these radionuclides determination and measurement in a low background proportional detector. The results showed that Parque das Águas de Lambari presented highest concentration values when compared with Contendas. In Parque das Águas de Contendas , the highest concentration values were 77 ± 5 mBq/L and 129 ± 5 mBq/L for "2"2"6Ra for Gasosa II springs and "2"2"8Ra for Gasosa I spring, respectively, which correspond to the spring season; for "2"1"0Pb, 27 ± 2 mBq/L for Magnesiana spring in the same season. In Parque das Águas de Lambari, the highest concentration values for "2"2"6Ra was 177 ± 2 mBq/L and for "2"1"0Pb, 36 ± 2 mBq/L, both in the Magnesiana spring, for the summer season and 135 ± 5 mBq/L for "2"2"8Ra, in Magnesiana spring, for the spring season. The data were analyzed through descriptive and correlational statistical techniques. Therefore, it was possible to evaluate the committed effective doses due to the consumption of these waters and to assess the radiological risk for the radionuclides studied. (author)

  18. Estudo do reúso não potável de água de processo e efluente tratado em indústria de bebidas

    OpenAIRE

    Galvão, Michel Ribas

    2011-01-01

    Resumo:O rigor da legislação atual sobre o processo de outorga e cobrança de captação de água e lançamento de efluente tratado, além da perda e desperdício de água durante o processo produtivo, exige das indústrias soluções alternativas para o reúso de água e efluentes, visando a redução do uso de recursos naturais. O reúso não potável da água de processo possibilita uma menor dependência da utilização da água captada pela concessionária pública responsável pelo abastecimento da região. Uma d...

  19. Identification of Genes Upregulated by the Transcription Factor Bcr1 That Are Involved in Impermeability, Impenetrability, and Drug Resistance of Candida albicans a/α Biofilms

    Science.gov (United States)

    Srikantha, Thyagarajan; Daniels, Karla J.; Pujol, Claude; Kim, Elena

    2013-01-01

    Candida albicans forms two types of biofilm, depending upon the configuration of the mating type locus. Although architecturally similar, a/α biofilms are impermeable, impenetrable, and drug resistant, whereas a/a and α/α biofilms lack these traits. The difference appears to be the result of an alternative matrix. Overexpression in a/a cells of BCR1, a master regulator of the a/α matrix, conferred impermeability, impenetrability, and drug resistance to a/a biofilms. Deletion of BCR1 in a/α cells resulted in the loss of these a/α-specific biofilm traits. Using BCR1 overexpression in a/a cells, we screened 107 genes of interest and identified 8 that were upregulated by Bcr1. When each was overexpressed in a/a biofilms, the three a/α traits were partially conferred, and when each was deleted in a/α cells, the traits were partially lost. Five of the eight genes have been implicated in iron homeostasis, and six encode proteins that are either in the wall or plasma membrane or secreted. All six possess sites for O-linked and N-linked glycosylation that, like glycosylphosphatidylinositol (GPI) anchors, can cross-link to the wall and matrix, suggesting that they may exert a structural role in conferring impermeability, impenetrability, and drug resistance, in addition to their physiological functions. The fact that in a screen of 107 genes, all 8 of the Bcr1-upregulated genes identified play a role in impermeability, impenetrability, and drug resistance suggests that the formation of the a/α matrix is highly complex and involves a larger number of genes than the initial ones identified here. PMID:23563485

  20. Genes Involved in Initial Follicle Recruitment May Be Associated with Age at Menopause

    NARCIS (Netherlands)

    Voorhuis, Marlies; Broekmans, Frank J.; Fauser, Bart C. J. M.; Onland-Moret, N. Charlotte; van der Schouw, Yvonne T.

    Context: Timing of menopause is largely influenced by genetic factors. Because menopause occurs when the follicle pool in the ovaries has become exhausted, genes involved in primordial follicle recruitment can be considered as candidate genes for timing of menopause. Objective: The aim was to study

  1. Oxidação de microcistinas-LR em águas pelo íon ferrato(VI Aqueous oxidation of microcystin-LR by ferrate(VI

    Directory of Open Access Journals (Sweden)

    Sérgio João de Luca

    2010-03-01

    Full Text Available Toxinas de cianobactérias têm se tornado um grave problema na produção segura de água para consumo humano e animal. Técnicas convencionais de tratamento falham em atingir padrões de potabilidade. O ferrato(VI de potássio, um composto oxidante e coagulante, mostra potencialidade no tratamento de águas contaminadas. Neste trabalho, são apresentados resultados da oxidação pelo ferrato(VI de uma toxina gerada por cianobactérias, a microcistina-LR. Ensaios de cinética de oxidação e de teste de jarros mostram um valor médio de 0,012 min-1 para a constante de taxa de reação de pseudoprimeira ordem, para concentrações de MC-LR de 100 a 200 µg.L-1 na água bruta. Dosagens de 1,6 a 5,0 mg.L-1 de ferrato(VI sugerem o atendimento ao padrão de potabilidade para microcistinas, mostrando que o oxidante poderá ser empregado como coadjuvante no tratamento de água.Algae toxins are becoming a severe problem in the water treatment industry, especially for human and animal consumption. Traditional treatment processes have failed in complying with water supply standards. Potassium ferrate(VI is a powerful oxidant, disinfectant and, also, a coagulant. In this paper, the results of microcystin-LR oxidation by ferrate(VI ion are presented. Kinetic and jar tests showed a average value of 0,012 min-1 for the pseudo first order reaction rate constant, for 100 and 200 µg.L-1 concentration of MC-LR. Ferrate(VI dosages between 1.6 and 5.0 mg.L-1 suggest that water supply standards for MC-LR can be reached, which means that the oxidant may be employed as coadjuvant in water treatment.

  2. Aplicação para o estudo do aproveitamento de águas pluviais para usos urbanos não potáveis

    OpenAIRE

    Oliveira, F. S. B. F.; Almeida, M. C.; Leitão, J. P.

    2012-01-01

    A recolha e utilização das águas pluviais embora tenha sido utilizada em Portugal, em diversas zonas do país, foi sendo abandonada devido à expansão generalizada dos sistemas de abastecimento de água. No entanto, num enquadramento de uso mais eficiente da água, considerando a adequação da qualidade ao tipo de uso, o aproveitamento local da água pluvial é cada vez mais encarado como uma medida adequada. Neste artigo é apresentada uma aplicação para avaliar os benefícios da insta...

  3. QUALIDADE DA ÁGUA DE CHUVA ARMAZENADA EM CISTERNAS RURAIS E AS MODIFICAÇÕES DECORRENTES DO MANUSEIO NA REGIÃO DE SERRINHA-BA.

    Directory of Open Access Journals (Sweden)

    Maria Auxiliadora Freitas dos Santos

    2015-01-01

    Full Text Available No nordeste brasileiro, em especial, nas áreas áridas, a escassez de água para o consumohumano constitui-se uma realidade comum. Dentre as propostas de aproveitamento,utilização e armazenamento da água da chuva, tem-se as cisternas rurais. Este trabalhoteve como objetivo avaliar a qualidade da água da chuva armazenada em cisternas ruraisassociada às práticas de manuseio realizadas pelas famílias no município deSerrinha/BA. No período de outubro/2007 a janeiro/2008 foram realizadas análisesbacteriológicas e físico-químicas das amostras de água armazenadas nas cisternas,associando-as aos dados relacionados sobre captação armazenamento, uso e manuseiodesta água através da aplicação de formulário. Os resultados demonstraram que, emrelação aos parâmetros bacteriológicos, todas as amostras apresentaram E.c. Quanto aosparâmetros físico-químicos, aqueles que estavam fora dos limites estabelecidos pelaportaria n518/04, foram: 27,8% em relação a cor, 4,2% em relação a turbidez. Emrelação aos parâmetros pH, sulfato, dureza, alcalinidade, cloreto, sólidos dissolvidos,nitrito e nitrato as amostras estão em conformidade com a portaria citada. Foramidentificadas algumas práticas de armazenamento e manuseio que proporcionam acontaminação da água, dentre elas: 44,4% das famílias entrevistadas misturam a água dachuva com água de outras fontes, 11,2% não desviam a primeira água da chuva, 27,8 %insere peixes na água e deste percentual, 20% fornece alimento. Para captar a água,70,8% das famílias utilizam o balde. A utilização de práticas inadequadas possibilitou oaumento da concentração de E.c e entre as famílias que apenas utilizam a bombamanual foram encontrados os menores valores de E.c. As cisternas constituem umatecnologia viável para o abastecimento de água no nordeste brasileiro, entretanto, asfamílias devem aperfeiçoar as técnicas de manejo, dentre elas realizar a desinfecção daágua da chuva

  4. Genes involved in meso-diaminopimelate synthesis in Bacillus subtilis: identification of the gene encoding aspartokinase I.

    Science.gov (United States)

    Roten, C A; Brandt, C; Karamata, D

    1991-04-01

    Thermosensitive mutants of Bacillus subtilis deficient in peptidoglycan synthesis were screened for mutations in the meso-diaminopimelate (LD-A2pm) metabolic pathway. Mutations in two out of five relevant linkage groups, lssB and lssD, were shown to induce, at the restrictive temperature, a deficiency in LD-A2pm synthesis and accumulation of UDP-MurNAc-dipeptide. Group lssB is heterogeneous; it encompasses mutations that confer deficiency in the deacylation of N-acetyl-LL-A2pm and accumulation of this precursor. Accordingly, these mutations are assigned to the previously identified locus dapE. Mutations in linkage group lssD entail a thermosensitive aspartokinase 1. Therefore, they are most likely to affect the structural gene of this enzyme, which we propose to designate dapG. Mutation pyc-1476, previously reported to affect the pyruvate carboxylase, was shown to confer a deficiency in aspartokinase 1, not in the carboxylase, and to belong to the dapG locus, dapG is closely linked to spoVF, the putative gene of dipicolinate synthase. In conclusion, mutations affecting only two out of eight steps known to be involved in LD-A2pm synthesis were uncovered in a large collection of thermosensitive mutants obtained by indirect selection. We propose that this surprisingly restricted distribution of the thermosensitive dap mutations isolated so far is due to the existence, in each step of the pathway, of isoenzymes encoded by separate genes. The biological role of different aspartokinases was investigated with mutants deficient in dapE and dapG genes. Growth characteristics of these mutants in the presence of various combinations of aspartate family amino acids allow a reassessment of a metabolic channel hypothesis, i.e. the proposed existence of multienzyme complexes, each specific for a given end product.

  5. Análise sensorial da água de coco anão verde processada e comercializada no sertão paraibano e cearense

    Directory of Open Access Journals (Sweden)

    Suziane Alves Josino Lima

    2015-06-01

    Full Text Available O Brasil, atualmente, possui uma grande tendência de crescimento do cultivo do coqueiro anão verde, onde o principal objetivo deste se da para a produção da água de coco, sua cultura esta distribuída em todo o território nacional, destacadamente na região Nordeste onde se concentram os maiores plantios do país. A água de coco é um produto natural que vem ganhando espaço no mercado como uma bebida de vasto potencial comercial, pois além de ser um produto natural é praticamente livre de contaminações, possui baixo teor calórico, considerável valor nutricional e apresenta aroma e sabor suaves e agradáveis. Neste contexto se faz necessário a avaliação da qualidade sensorial da água de coco industrializada e comercializada de forma a garantir a qualidade do produto para o consumidor. Com isso este trabalho objetivou avaliar sensorialmente á água de coco anão verde, produzidas comercialmente e envasadas, por indústrias do Sertão Paraibano e do Ceará. As amostras de água de coco proveniente das indústrias, foram analisadas sensorialmente no laboratório de microbiologia do CVT – UFCG - Pombal, utilizando o teste de comparação múltiplas utilizando uma escala hedônica de 9 pontos (9 = gostei extremamente, até 1 desgostei, também foi aplicado o teste de intenção de compra utilizando-se uma escala estruturada de 5 pontos (1 = certamente compraria, até 5 = Certamente compraria, conforme metodologia do Instituto Adolfo Lutz. Os resultados dos testes somente se deram até o 15 dias de armazenamento, onde 70% dos copos de água de coco armazenados, apresentaram-se turvos e com produção de gás, demonstrando apartir deste ponto impróprios para o consumo. Com 30 dias de armazenamento todas as amostras apresentaram insatisfatórios para consumo, demonstrando resultados preocupantes quanto a estabilidade da qualidade do produto durante sua validade comercial. Sugere-se como forma de garantia e manutenção da qualidade

  6. Avaliação integrada da qualidade química e da genotoxicidade da água do arroio Luiz Rau, no trecho inferior da Bacia do Rio dos Sinos, no Sul do Brasil

    OpenAIRE

    Petry, Camila Tamires; Costa, Gustavo Marques da; Benvenuti, Tatiane; Rodrigues, Marco Antônio Siqueira; Droste, Annette

    2016-01-01

    A qualidade química e a genotoxicidade da água do arroio Luiz Rau em Novo Hamburgo (Rio Grande do Sul, Brasil) e a relação entre os danos genéticos observados em Tradescantia pallida var. purpurea e parâmetros químicos foram analisados. Amostras de água foram coletadas bimestralmente, entre setembro de 2012 e março de 2013 em dois sítios: próximo à nascente (S1) e próximo à foz (S2) do arroio. Ramos com botões florais foram expostos à água dos sítios e à água destilada (controle negativo). Fr...

  7. sugE: A gene involved in tributyltin (TBT) resistance of Aeromonas molluscorum Av27.

    Science.gov (United States)

    Cruz, Andreia; Micaelo, Nuno; Félix, Vitor; Song, Jun-Young; Kitamura, Shin-Ichi; Suzuki, Satoru; Mendo, Sónia

    2013-01-01

    The mechanism of bacterial resistance to tributyltin (TBT) is still unclear. The results herein presented contribute to clarify that mechanism in the TBT-resistant bacterium Aeromonas molluscorum Av27. We have identified and cloned a new gene that is involved in TBT resistance in this strain. The gene is highly homologous (84%) to the Aeromonas hydrophila-sugE gene belonging to the small multidrug resistance gene family (SMR), which includes genes involved in the transport of lipophilic drugs. In Av27, expression of the Av27-sugE was observed at the early logarithmic growth phase in the presence of a high TBT concentration (500 μM), thus suggesting the contribution of this gene for TBT resistance. E. coli cells transformed with Av27-sugE become resistant to ethidium bromide (EtBr), chloramphenicol (CP) and tetracycline (TE), besides TBT. According to the Moriguchi logP (miLogP) values, EtBr, CP and TE have similar properties and are substrates for the sugE-efflux system. Despite the different miLogP of TBT, E. coli cells transformed with Av27-sugE become resistant to this compound. So it seems that TBT is also a substrate for the SugE protein. The modelling studies performed also support this hypothesis. The data herein presented clearly indicate that sugE is involved in TBT resistance of this bacterium.

  8. Avaliação da concentração de flúor e do consumo de água mineral

    OpenAIRE

    Ramires, Irene; Grec, Roberto Henrique da Costa; Cattan, Lourenço; Moura, Patrícia Gomes de; Lauris, José Roberto Pereira; Buzalaf, Marília Afonso Rabelo

    2004-01-01

    OBJETIVO: Considerando a água como importante fonte de ingestão de flúor, o aumento da prevalência de fluorose dentária e do consumo de água mineral, o estudo realizado teve como objetivo avaliar o consumo de água mineral e sua concentração de flúor. MÉTODOS: A pesquisa foi realizada nos bairros do município de Bauru, SP. Foram visitadas mil residências que compunham uma amostra estratificada, obtida por conglomerados. Cada conglomerado correspondia a um quarteirão. Para efeito de sorteio os ...

  9. Uso do res?duo da esta??o de tratamento de ?gua de Ji-Paran?/RO como material ciment?cio

    OpenAIRE

    Rocha, Maria Angelica Froes da

    2016-01-01

    O lodo ? um subproduto inevit?vel no tratamento da ?gua a ser fornecida a uma popula??o, sendo que a sua disposi??o final muitas vezes ? realizada de forma inadequada e n?o sustent?vel. A argamassa ? uma mistura de cimento, areia e ?gua. Este trabalho tem o objetivo de avaliar a substitui??o parcial do agregado mi?do pelo lodo produzido na esta??o de tratamento de ?gua de Ji-Paran? na produ??o de argamassa. A metodologia utilizada no presente estudo consistiu na coleta e secagem do lodo, cara...

  10. Surto de reações hemolíticas associado a residuais de cloro e cloraminas na água de hemodiálise

    Directory of Open Access Journals (Sweden)

    Calderaro Rachel VV

    2001-01-01

    Full Text Available OBJETIVO: Relatar o processo de investigação da contaminação da água e a conseqüente avaliação do surto ocorrido no serviço de hemodiálise. MÉTODOS: Em setembro de 2000, 16 pacientes sob terapia de hemodiálise de um hospital em Minas Gerais apresentaram reações hemolíticas compatíveis a sintomas de intoxicação por cloro e cloramina em água. Foi feita a medição das concentrações de cloro e cloramina em amostras coletadas em diversos pontos do sistema de tratamento e distribuição de água do serviço inspecionado. A identificação dos casos ocorridos durante o período de estudo foi feita pela revisão das anotações de prontuários dos pacientes. Foi feita a revisão dos procedimentos da equipe técnica, médica e de enfermagem por meio de entrevistas. RESULTADOS: A taxa de sintomas foi significativamente alta (p<0,028 durante o período epidêmico em relação ao período pré-epidêmico. Os pacientes afetados fizeram hemodiálise com água tratada por osmose reversa e usaram capilares dialisadores reprocessados manualmente. A água e os capilares apresentaram, durante o período epidêmico, concentrações residuais acima dos padrões desejáveis impostos pela Portaria 82 do Ministério da Saúde/GM, que exige o máximo de 0,5 mg/l para cloro e 0,1 mg/l para cloramina. Risco relativo de 2,58 (1,06 a 6,28 caracteriza elevadas concentrações de cloro livre e cloramina quanto à apresentação dos sintomas nos expostos. CONCLUSÃO: A vigilância dos procedimentos é necessária para que a água utilizada no processo de hemodiálise atenda aos padrões mínimos recomendados.

  11. Surto de reações hemolíticas associado a residuais de cloro e cloraminas na água de hemodiálise

    Directory of Open Access Journals (Sweden)

    Rachel VV Calderaro

    2001-10-01

    Full Text Available OBJETIVO: Relatar o processo de investigação da contaminação da água e a conseqüente avaliação do surto ocorrido no serviço de hemodiálise. MÉTODOS: Em setembro de 2000, 16 pacientes sob terapia de hemodiálise de um hospital em Minas Gerais apresentaram reações hemolíticas compatíveis a sintomas de intoxicação por cloro e cloramina em água. Foi feita a medição das concentrações de cloro e cloramina em amostras coletadas em diversos pontos do sistema de tratamento e distribuição de água do serviço inspecionado. A identificação dos casos ocorridos durante o período de estudo foi feita pela revisão das anotações de prontuários dos pacientes. Foi feita a revisão dos procedimentos da equipe técnica, médica e de enfermagem por meio de entrevistas. RESULTADOS: A taxa de sintomas foi significativamente alta (p<0,028 durante o período epidêmico em relação ao período pré-epidêmico. Os pacientes afetados fizeram hemodiálise com água tratada por osmose reversa e usaram capilares dialisadores reprocessados manualmente. A água e os capilares apresentaram, durante o período epidêmico, concentrações residuais acima dos padrões desejáveis impostos pela Portaria 82 do Ministério da Saúde/GM, que exige o máximo de 0,5 mg/l para cloro e 0,1 mg/l para cloramina. Risco relativo de 2,58 (1,06 a 6,28 caracteriza elevadas concentrações de cloro livre e cloramina quanto à apresentação dos sintomas nos expostos. CONCLUSÃO: A vigilância dos procedimentos é necessária para que a água utilizada no processo de hemodiálise atenda aos padrões mínimos recomendados.

  12. Early-Onset Alzheimer Disease and Candidate Risk Genes Involved in Endolysosomal Transport.

    Science.gov (United States)

    Kunkle, Brian W; Vardarajan, Badri N; Naj, Adam C; Whitehead, Patrice L; Rolati, Sophie; Slifer, Susan; Carney, Regina M; Cuccaro, Michael L; Vance, Jeffery M; Gilbert, John R; Wang, Li-San; Farrer, Lindsay A; Reitz, Christiane; Haines, Jonathan L; Beecham, Gary W; Martin, Eden R; Schellenberg, Gerard D; Mayeux, Richard P; Pericak-Vance, Margaret A

    2017-09-01

    gene RIN3 showed suggestive evidence of association with EOAD after Bonferroni correction (OR, 4.56; 95% CI, 1.26-16.48; P = .02, BP = 0.091). In addition, a missense variant in RUFY1 identified in 2 NHW EOAD cases showed suggestive evidence of an association with EOAD as well (OR, 18.63; 95% CI, 1.62-213.45; P = .003; BP = 0.129). The genes PSD2, TCIRG1, RIN3, and RUFY1 all may be involved in endolysosomal transport-a process known to be important to development of AD. Furthermore, this study identified shared risk genes between EOAD and LOAD similar to previously reported genes, such as SORL1, PSEN2, and TREM2.

  13. Non-canonical PRC1.1 Targets Active Genes Independent of H3K27me3 and Is Essential for Leukemogenesis

    Directory of Open Access Journals (Sweden)

    Vincent van den Boom

    2016-01-01

    Full Text Available Polycomb proteins are classical regulators of stem cell self-renewal and cell lineage commitment and are frequently deregulated in cancer. Here, we find that the non-canonical PRC1.1 complex, as identified by mass-spectrometry-based proteomics, is critically important for human leukemic stem cells. Downmodulation of PRC1.1 complex members, like the DNA-binding subunit KDM2B, strongly reduces cell proliferation in vitro and delays or even abrogates leukemogenesis in vivo in humanized xenograft models. PRC1.1 components are significantly overexpressed in primary AML CD34+ cells. Besides a set of genes that is targeted by PRC1 and PRC2, ChIP-seq studies show that PRC1.1 also binds a distinct set of genes that are devoid of H3K27me3, suggesting a gene-regulatory role independent of PRC2. This set encompasses genes involved in metabolism, which have transcriptionally active chromatin profiles. These data indicate that PRC1.1 controls specific genes involved in unique cell biological processes required for leukemic cell viability.

  14. FoxO1 and HNF-4 are involved in regulation of hepatic glucokinase gene expression by resveratrol.

    Science.gov (United States)

    Ganjam, Goutham Kumar; Dimova, Elitsa Y; Unterman, Terry G; Kietzmann, Thomas

    2009-11-06

    Resveratrol, a polyphenol derived from grapes, exerts important effects on glucose and lipid metabolism, yet detailed mechanisms mediating these effects remain unknown. The liver plays a central role in energy homeostasis, and glucokinase (GK) is a key enzyme involved in glucose utilization. Resveratrol activates SIRT1 (sirtuin 1), which promotes deacetylation of the forkhead transcription factor FoxO1. Previously, we reported that FoxO1 can suppress and that HNF-4 can stimulate GK expression in the liver. Here, we examined the role of FoxO1 and HNF-4 in mediating resveratrol effects on liver GK expression. Resveratrol suppressed hepatic GK expression in vivo and in isolated hepatocytes, and knocking down FoxO1 with shRNAs disrupted this effect. Reporter gene, gel shift, supershift assay, and chromatin immunoprecipitation studies show that FoxO1 binds to the GK promoter and that the interplay between FoxO1 and HNF-4 within the GK promoter is essential for mediating the effects of resveratrol. Resveratrol promotes deacetylation of FoxO1 and enhances its recruitment to the FoxO-binding element. Conversely, resveratrol suppresses recruitment of HNF-4 to its binding site, and knockdown of FoxO1 blocks this effect of resveratrol. Coprecipitation and chromatin immunoprecipitation studies show that resveratrol enhances interaction between FoxO1 and HNF-4, reduces binding of HNF-4 to its own site, and promotes its recruitment to the FoxO site in a FoxO1-dependent manner. These results provide the first evidence that resveratrol represses GK expression via FoxO1 and that the interaction between FoxO1 and HNF-4 contributes to these effects of resveratrol.

  15. A human repair gene ERCC5 is involved in group G xeroderma pigmentosum

    International Nuclear Information System (INIS)

    Shiomi, Tadahiro

    1994-01-01

    In E. coli, ultraviolet-induced DNA damage is removed by the coordinated action of UVR A, B, C, and D proteins (1). In Saccharomyces cerevisiae, more than ten genes have been reported to be involved in excision repair (2). The nucleotide excision repair pathway has been extensively studied in these organisms. To facilitate studying nucleotide excision repair in mammalian cells. Ultraviolet-sensitive rodent cell mutants have been isolated and classified into 11 complementation groups (9,10). The human nucleotide excision repair genes which complement the defects of the mutants have been designated as the ERCC (excision repair cross-complementing) genes; a number is added to refer to the particular rodent complementation group that is corrected by the gene. Recently, several human DNA repair genes have been cloned using rodent cell lines sensitive to ultraviolet. These include ERCC2 (3), ERCC3 (4), and ERCC6 (5), which correspond to the defective genes in the ultraviolet-sensitive human disorders xeroderma pigmentosum (XP) group D (6) and group B (4), and Cockayne's syndrome (CS) group B (7), respectively. The human excision repair gene ERCC5 was cloned after DNA-mediated gene transfer of human HeLa cell genomic DNA into the ultraviolet-sensitive mouse mutant XL216, a member of rodent complementation group 5 (11,12) and the gene was mapped on human chromosome 13q32.3-q33.1 by the replication R-banding fluorescence in situ hybridization method (13). The ERCC5 cDNA encodes a predicted 133 kDa nuclear protein that shares some homology with product of the yeast DNA repair gene RAD 2. Transfection with mouse ERCC5 cDNA restored normal levels of ultraviolet-resistance to XL216 cells. Microinjection of ERCC5 cDNA specifically restored the defect of XP group G cells (XP-G) as measured by unscheduled DNA synthesis (UDS), and XP-G cells stably transformed with ERCC5 cDNA showed nearly normal ultraviolet resistance. (J.P.N.)

  16. Identification of a novel gene in ROD9 island of Salmonella Enteritidis involved in the alteration of virulence-associated genes expression.

    Science.gov (United States)

    Das, Susmita; Ray, Shilpa; Ryan, Daniel; Sahu, Bikash; Suar, Mrutyunjay

    2018-01-01

    Salmonella enterica subsp. I serovar Enteritidis (S. Enteritidis), one of the causative agents for non-typhoidal gastrointestinal diseases in humans is an intracellular bacterium and mechanism for its invasion into host cells is critical to cause infection. The virulence of the pathogen is explained by the expression of genes located on its pathogenicity islands, mostly encoded under SPI-1 and SPI-2. However, S. Typhimurium SL1344, despite sharing ∼98% of its genome with S. Enteritidis P125109, lacks few regions of differences (ROD) that are hypothesized to impart virulence potential to S. Enteritidis. In this study, we created different mutants in the ROD9 island of S. Enteritidis, also referred as SPI-19 and identified a novel locus, SEN1005, encoding a hypothetical protein that is involved in its pathogenesis. ΔSEN1005 displayed significantly reduced entry into cultured epithelial cells as well as uptake by macrophages and failed to cause acute colitis in C57BL/6 mice at day 3 post-infection (p.i.). Additionally, the global transcriptome analysis revealed a highly repressed SPI-1 and other down-regulated genes responsible for flagellar assembly, chemotaxis and motility in the mutant which correlated with decreased invasion and abated inflammation as compared to the wild-type. Therefore, our findings revealed that ΔSEN1005 was attenuated in vitro as well as in vivo and we propose this hypothetical protein to play a role in altering the expression of genes involved in Salmonella virulence.

  17. Potencialidade de uso de fontes alternativas de água para fins não potáveis em uma unidade residencial

    OpenAIRE

    Peters, Madelon Rebelo

    2006-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pos-Graduação em Engenharia Ambiental. O uso racional da água compreende o controle de desperdícios e uma re-educação de hábitos e costumes. Esta re-educação está associada ao uso de fontes alternativas como, por exemplo, os efluentes tratados gerados na própria residência e a captação de águas pluviais, em substituição a água potável para fins que não seja o consumo humano. O uso de águas de c...

  18. Phenotypic Involvement in Females with the FMR1 Gene Mutation.

    Science.gov (United States)

    Riddle, J. E.; Cheema, A.; Sobesky, W. E.; Gardner, S. C.; Taylor, A. K.; Pennington, B. F.; Hagerman, R. J.

    1998-01-01

    A study investigated phenotypic effects seen in 114 females with premutation and 41 females (ages 18-58) with full Fragile X mental retardation gene mutation. Those with the full mutation had a greater incidence of hand-flapping, eye contact problems, special education help for reading and math, and grade retention. (Author/CR)

  19. Gene-environment interaction involving recently identified colorectal cancer susceptibility loci

    Science.gov (United States)

    Kantor, Elizabeth D.; Hutter, Carolyn M.; Minnier, Jessica; Berndt, Sonja I.; Brenner, Hermann; Caan, Bette J.; Campbell, Peter T.; Carlson, Christopher S.; Casey, Graham; Chan, Andrew T.; Chang-Claude, Jenny; Chanock, Stephen J.; Cotterchio, Michelle; Du, Mengmeng; Duggan, David; Fuchs, Charles S.; Giovannucci, Edward L.; Gong, Jian; Harrison, Tabitha A.; Hayes, Richard B.; Henderson, Brian E.; Hoffmeister, Michael; Hopper, John L.; Jenkins, Mark A.; Jiao, Shuo; Kolonel, Laurence N.; Le Marchand, Loic; Lemire, Mathieu; Ma, Jing; Newcomb, Polly A.; Ochs-Balcom, Heather M.; Pflugeisen, Bethann M.; Potter, John D.; Rudolph, Anja; Schoen, Robert E.; Seminara, Daniela; Slattery, Martha L.; Stelling, Deanna L.; Thomas, Fridtjof; Thornquist, Mark; Ulrich, Cornelia M.; Warnick, Greg S.; Zanke, Brent W.; Peters, Ulrike; Hsu, Li; White, Emily

    2014-01-01

    BACKGROUND Genome-wide association studies have identified several single nucleotide polymorphisms (SNPs) that are associated with risk of colorectal cancer (CRC). Prior research has evaluated the presence of gene-environment interaction involving the first 10 identified susceptibility loci, but little work has been conducted on interaction involving SNPs at recently identified susceptibility loci, including: rs10911251, rs6691170, rs6687758, rs11903757, rs10936599, rs647161, rs1321311, rs719725, rs1665650, rs3824999, rs7136702, rs11169552, rs59336, rs3217810, rs4925386, and rs2423279. METHODS Data on 9160 cases and 9280 controls from the Genetics and Epidemiology of Colorectal Cancer Consortium (GECCO) and Colon Cancer Family Registry (CCFR) were used to evaluate the presence of interaction involving the above-listed SNPs and sex, body mass index (BMI), alcohol consumption, smoking, aspirin use, post-menopausal hormone (PMH) use, as well as intake of dietary calcium, dietary fiber, dietary folate, red meat, processed meat, fruit, and vegetables. Interaction was evaluated using a fixed-effects meta-analysis of an efficient Empirical Bayes estimator, and permutation was used to account for multiple comparisons. RESULTS None of the permutation-adjusted p-values reached statistical significance. CONCLUSIONS The associations between recently identified genetic susceptibility loci and CRC are not strongly modified by sex, BMI, alcohol, smoking, aspirin, PMH use, and various dietary factors. IMPACT Results suggest no evidence of strong gene-environment interactions involving the recently identified 16 susceptibility loci for CRC taken one at a time. PMID:24994789

  20. Wounding coordinately induces cell wall protein, cell cycle and pectin methyl esterase genes involved in tuber closing layer and wound periderm development.

    Science.gov (United States)

    Neubauer, Jonathan D; Lulai, Edward C; Thompson, Asunta L; Suttle, Jeffrey C; Bolton, Melvin D

    2012-04-15

    Little is known about the coordinate induction of genes that may be involved in agriculturally important wound-healing events. In this study, wound-healing events were determined together with wound-induced expression profiles of selected cell cycle, cell wall protein, and pectin methyl esterase genes using two diverse potato genotypes and two harvests (NDTX4271-5R and Russet Burbank tubers; 2008 and 2009 harvests). By 5 d after wounding, the closing layer and a nascent phellogen had formed. Phellogen cell divisions generated phellem layers until cessation of cell division at 28 d after wounding for both genotypes and harvests. Cell cycle genes encoding epidermal growth factor binding protein (StEBP), cyclin-dependent kinase B (StCDKB) and cyclin-dependent kinase regulatory subunit (StCKS1At) were induced by 1 d after wounding; these expressions coordinated with related phellogen formation and the induction and cessation of phellem cell formation. Genes encoding the structural cell wall proteins extensin (StExt1) and extensin-like (StExtlk) were dramatically up-regulated by 1-5 d after wounding, suggesting involvement with closing layer and later phellem cell layer formation. Wounding up-regulated pectin methyl esterase genes (StPME and StPrePME); StPME expression increased during closing layer and phellem cell formation, whereas maximum expression of StPrePME occurred at 5-14 d after wounding, implicating involvement in later modifications for closing layer and phellem cell formation. The coordinate induction and expression profile of StTLRP, a gene encoding a cell wall strengthening "tyrosine-and lysine-rich protein," suggested a role in the formation of the closing layer followed by phellem cell generation and maturation. Collectively, the genes monitored were wound-inducible and their expression profiles markedly coordinated with closing layer formation and the index for phellogen layer meristematic activity during wound periderm development; results were more

  1. Maturidade de potros nascidos de éguas com placentite

    Directory of Open Access Journals (Sweden)

    L.S. Feijó

    2014-12-01

    Full Text Available Objetivou-se descrever a maturidade neonatal através da resposta clínica, comportamental e hematológica de potros nascidos de éguas com placentite. Participaram do estudo seis potros nascidos de éguas submetidas à indução experimental de placentite ascendente através da infusão intracervical de Streptococcus equi subespécie zooepidemicus e tratadas com Sulfa-trimetoprim e Flunixin meglumine. A formação dos grupos neonatais foi realizada de acordo com o grau de viabilidade e sobrevivência até 60 horas: Grupo Não Sobreviventes (n=2; Grupo Debilitados (n=2; Grupo Saudáveis (n=2. Foi considerado o tempo de gestação, período de intervalo inoculação-parto, avaliação comportamental, clínica e hematológica. O Grupo dos potros Saudáveis apresentou maior tempo de gestação (320±2 dias e maior intervalo inoculação-parto (20,5±2,5 dias. Os Grupos Não Sobreviventes e Debilitados apresentaram atraso para decúbito esternal e reflexo de sucção. Foi observada bradicardia e hipotermia com 48h de vida no Grupo Não Sobreviventes. Os potros do Grupo Não Sobreviventes e Saudáveis apresentaram leucopenia no nascimento com discretas variações até as 48h. Os potros nascidos de éguas com placentite ascendente e tratadas demonstraram evolução clínica e respostas neonatais distintas. Conclui-se que, quanto maior o tempo de manutenção da gestação após a injúria placentária, melhor será a maturação fetal, o que refletirá em viabilidade e melhor capacidade de resposta neonatal.

  2. Involvement of Three Esterase Genes from Panonychus citri (McGregor in Fenpropathrin Resistance

    Directory of Open Access Journals (Sweden)

    Xiao-Min Shen

    2016-08-01

    Full Text Available The citrus red mite, Panonychus citri (McGregor, is a major citrus pest with a worldwide distribution and an extensive record of pesticide resistance. However, the underlying molecular mechanism associated with fenpropathrin resistance in this species have not yet been reported. In this study, synergist triphenyl phosphate (TPP dramatically increased the toxicity of fenpropathrin, suggesting involvement of carboxylesterases (CarEs in the metabolic detoxification of this insecticide. The subsequent spatiotemporal expression pattern analysis of PcE1, PcE7 and PcE9 showed that three CarEs genes were all over-expressed after insecticide exposure and higher transcripts levels were observed in different field resistant strains of P. citri. Heterologous expression combined with 3-(4,5-dimethyl-thiazol-2-yl-2,5-diphenyltetra-zolium bromide (MTT cytotoxicity assay in Spodoptera frugiperda (Sf9 cells revealed that PcE1-, PcE7- or PcE9-expressing cells showed significantly higher cytoprotective capability than parental Sf9 cells against fenpropathrin, demonstrating that PcEs probably detoxify fenpropathrin. Moreover, gene silencing through the method of leaf-mediated dsRNA feeding followed by insecticide bioassay increased the mortalities of fenpropathrin-treated mites by 31% (PcE1, 27% (PcE7 and 22% (PcE9, respectively, after individual PcE gene dsRNA treatment. In conclusion, this study provides evidence that PcE1, PcE7 and PcE9 are functional genes mediated in fenpropathrin resistance in P. citri and enrich molecular understanding of CarEs during the resistance development of the mite.

  3. An ortholog of farA of Aspergillus nidulans is implicated in the transcriptional activation of genes involved in fatty acid utilization in the yeast Yarrowia lipolytica

    International Nuclear Information System (INIS)

    Poopanitpan, Napapol; Kobayashi, Satoshi; Fukuda, Ryouichi; Horiuchi, Hiroyuki; Ohta, Akinori

    2010-01-01

    Research highlights: → POR1 is a Yarrowia lipolytica ortholog of farA involved in fatty acid response in A. nidulans. → Deletion of POR1 caused growth defects on fatty acids. → Δpor1 strain exhibited defects in the induction of genes involved in fatty acid utilization. -- Abstract: The yeast Yarrowia lipolytica effectively utilizes hydrophobic substrates such as fatty acids and n-alkanes. To identify a gene(s) regulating fatty acid utilization in Y. lipolytica, we first studied homologous genes to OAF1 and PIP2 of Saccharomyces cerevisiae, but their disruption did not change growth on oleic acid at all. We next characterized a Y. lipolytica gene, POR1 (primary oleate regulator 1), an ortholog of farA encoding a transcriptional activator that regulates fatty acid utilization in Aspergillus nidulans. The deletion mutant of POR1 was defective in the growth on various fatty acids, but not on glucose, glycerol, or n-hexadecane. It exhibited slight defect on n-decane. The transcriptional induction of genes involved in β-oxidation and peroxisome proliferation by oleate was distinctly diminished in the Δpor1 strains. These data suggest that POR1 encodes a transcriptional activator widely regulating fatty acid metabolism in Y. lipolytica.

  4. Estudo do resfriamento de carcaças de frango em chiller de imersão em água

    OpenAIRE

    Carciofi, Bruno Augusto Mattar

    2005-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia de Alimentos O resfriamento industrial de carcaças de frango é normalmente efetuado em tanques de imersão em água (chillers). Nesta etapa, além da redução da temperatura, ocorre absorção de água pelas carcaças. Neste trabalho foram estudados diversos aspectos relacionados com a redução da temperatura e com a absorção de água por carcaças resfriadas por imersão, além ...

  5. O CICLO DAS ÁGUAS DE MOACYR SCLIAR À LUZ DA HERMENÊUTICA SIMBÓLICA O CICLO DAS ÁGUAS BY MOACYR SCLIAR IN THE LIGHT OF SYMBOLIC HERMENEUTICS

    Directory of Open Access Journals (Sweden)

    Suzana Yolanda Lenhardt Machado Cánovas

    2011-02-01

    Full Text Available Este trabalho tem como objetivo estudar as imagens da água na narrativa O ciclo das águas, de Moacyr Scliar. Buscamos comprovar que o texto apresenta componentes míticos análogos aos das sociedades primitivas e arcaicas, ainda que pertença a um contexto realista. Isso permite que o aproximemos de poemas e relatos antigos. Mas como a obra de Scliar pertence a um mundo desprovido de significação religiosa, é frequente a desconstrução dos mitos, fato que é responsável pela natureza parodística do texto. Utilizamos concepções de Mircea Eliade, Northrop Frye e José Lorite Mena.This article aims at studying the cyclical images of water in the novel O ciclo das Águas, by Moacyr Scliar. We intend to prove that, even though the work belongs to a realistic context, it presents mythical components similar to primitive and archaic societies, which allows us to approach the novel to poems and ancient reports. However, as the novel by Scliar belongs to a world without religious meaning, it is frequent to observe the deconstruction of myths, which is responsible for the parody intrinsic to the text.

  6. Molecular characterization of the porcine deleted in malignant brain tumors 1 gene (DMBT1)

    DEFF Research Database (Denmark)

    Haase, Bianca; Humphray, Sean J; Lyer, Stefan

    2006-01-01

    The human gene deleted in malignant brain tumors 1 (DMBT1) is considered to play a role in tumorigenesis and pathogen defense. It encodes a protein with multiple scavenger receptor cysteine-rich (SRCR) domains, which are involved in recognition and binding of a broad spectrum of bacterial pathogens...

  7. Caracterização e aproveitamento de água cinza para uso não-potável em edificações

    OpenAIRE

    Bazzarella, Bianca Barcellos

    2005-01-01

    Uma das formas de se conservar água nas residências é utilizando fontes alternativas de suprimento. O reúso de águas cinza para fins não-potáveis, tais como lavagem de veículos, rega de jardins e descarga de vasos sanitários, é apenas um exemplo. Este trabalho tem como objetivo pesquisar alternativas para redução do consumo de água potável e da produção de esgotos sanitários em edificações, a partir do aproveitamento do tratamento e reúso de águas cinza em descargas de vasos sanitários. Pa...

  8. Accumulation of catechins in tea in relation to accumulation of mRNA from genes involved in catechin biosynthesis.

    Science.gov (United States)

    Eungwanichayapant, P D; Popluechai, S

    2009-02-01

    Catechins are a group of polyphenols found in tea (Camellia sinensis var. sinensis) at high levels. They are beneficial for health. From the study on accumulation of catechins in shoots and mature leaves of a tea cultivar, Oolong No. 17, using high-performance liquid chromatography (HPLC), it was found that the amounts of most catechins in the shoots were higher than those in the mature leaves, with an exception of catechins gallate (CG) that was found in trace amounts in both the shoots and mature leaves. mRNA accumulation of genes involved in catechin synthesis was studied using reverse transcriptase-polymerase chain reaction (RT-PCR). The results showed that the mRNA accumulation of the genes were higher in the shoots than in the mature leaves. These genes included genes of phenylalanine ammonia-lyase 1 (PAL1; EC 4.3.1.5), chalcone synthase (CHS; EC 2.3.1.74), dihydroflavonol 4-reductase (DFR; EC 1.1.1.219), leucoanthocyanidin reductase (LCR; EC 1.17.1.3), and flavanone 3-hydroxylase (F3H; EC 1.14.11.9).

  9. Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes.

    Science.gov (United States)

    Welker, Noah C; Habig, Jeffrey W; Bass, Brenda L

    2007-07-01

    We describe the first microarray analysis of a whole animal containing a mutation in the Dicer gene. We used adult Caenorhabditis elegans and, to distinguish among different roles of Dicer, we also performed microarray analyses of animals with mutations in rde-4 and rde-1, which are involved in silencing by siRNA, but not miRNA. Surprisingly, we find that the X chromosome is greatly enriched for genes regulated by Dicer. Comparison of all three microarray data sets indicates the majority of Dicer-regulated genes are not dependent on RDE-4 or RDE-1, including the X-linked genes. However, all three data sets are enriched in genes important for innate immunity and, specifically, show increased expression of innate immunity genes.

  10. Águas minerais de algumas fontes naturais brasileiras Mineral waters from brazilian natural sources

    Directory of Open Access Journals (Sweden)

    M. A. P. Rebelo

    1999-07-01

    Full Text Available Paciente de litíase renal é estimulado a aumentar a ingestão hídrica, principalmente na forma de água tratada da torneira, pela irrelevante biodisponibilidade de minerais em sua composição. O benefício é atribuído à redução da saturação dos sais urinários. Não está claro se as águas minerais naturais comercialmente disponíveis também é benéfica. OBJETIVO. Divulgar as informações sobre as características químicas e físico-químicas de águas minerais naturais de algumas das diversas fontes brasileiras. Essas informações serão de utilidade na análise de protocolos de pesquisa e na orientação do paciente. MÉTODOS. Foi feito uma coletânea das informações que constam nos rótulos de garrafas de águas minerais naturais, predominantemente não-gasosas, acessíveis ao consumo carioca. A partir da composição salina provável, foi calculada a concentração iônica de cada mineral. RESULTADOS. Foram estudadas as informações de 36 fontes situadas em alguns estados brasileiros. O pH, a 25oC, variou de 4,1 a 9,3 na dependência da fonte e foi linearmente correlacionado com as concentrações dos cátions cálcio, magnésio, sódio e do anion bicarbonato. Isto foi atribuído à alta alcalinidade desses sais, que contêm cerca de 70% de bicarbonato na molécula. Os teores de cálcio (0,3 a 42 mg/l, de magnésio (0,0 a 18 mg/l e de bicarbonato (4 a 228 mg/l foram considerados relativamente baixos. CONCLUSÃO. O teor mineral das fontes brasileiras que compuseram esse levantamento é baixo, com cerca de 70% delas apresentando teores de cálcio e de magnésio abaixo de 10 mg/l e 1 mg/l, respectivamente, semelhantes ao da água tratada da torneira.PURPOSE. To divulge information on the chemical composition and physical-chemical features of some mineral waters from Brazilian natural sources that will be of useful protocol in vestigation and patient advice. METHODS. The survey was based on bottle labels of non

  11. Genetic disruption of the KLF1 gene to overexpress the γ-globin gene using the CRISPR/Cas9 system.

    Science.gov (United States)

    Shariati, Laleh; Khanahmad, Hossein; Salehi, Mansoor; Hejazi, Zahra; Rahimmanesh, Ilnaz; Tabatabaiefar, Mohammad Amin; Modarressi, Mohammad Hossein

    2016-10-01

    β-thalassemia comprises a major group of human genetic disorders involving a decrease in or an end to the normal synthesis of the β-globin chains of hemoglobin. KLF1 is a key regulatory molecule involved in the γ- to β-globin gene switching process directly inducing the expression of the β-globin gene and indirectly repressing γ-globin. The present study aimed to investigate the ability of an engineered CRISPR/Cas9 system with respect to disrupting the KLF1 gene to inhibit the γ- to β-hemoglobin switching process in K562 cells. We targeted three sites on the KLF1 gene, two of which are upstream of codon 288 in exon 2 and the other site being in exon 3. The average indel percentage in the cells transfected with CRISPR a, b and c was approximately 24%. Relative quantification was performed for the assessment of γ-globin expression. The levels of γ-globin mRNA on day 5 of differentiation were 8.1-, 7.7- and 1.8-fold in the cells treated with CRISPR/Cas9 a, b and c, respectively,compared to untreated cells. The measurement of HbF expression levels confirmed the same results. The findings obtained in the present study support the induction of an indel mutation in the KLF1 gene leading to a null allele. As a result, the effect of KLF1 on the expression of BCL11A is decreased and its inhibitory effect on γ-globin gene expression is removed. Application of CRISPR technology to induce an indel in the KLF1 gene in adult erythroid progenitors may provide a method for activating fetal hemoglobin expression in individuals with β-thalassemia or sickle cell disease. Copyright © 2016 John Wiley & Sons, Ltd.

  12. Variation in the PTEN-induced putative kinase 1 gene associated ...

    Indian Academy of Sciences (India)

    with the increase risk of type 2 diabetes in northern Chinese. YANCHUN QU1†∗ ... and genetic variation analysis have indicated the involvement of PINK1 gene in the ... Qualitative variables were analysed by a chi-squared test. The level of ...

  13. Microbiological quality of drinking water of urban and rural communities, Brazil Qualidade microbiológica de água potável de comunidades urbanas e rurais, Paraná

    Directory of Open Access Journals (Sweden)

    Giovani Nogueira

    2003-04-01

    Full Text Available OBJECTIVE: To evaluate the microbiological quality of treated and untreated water samples came from urban and rural communities and to examine the relationship between coliforms occurrence and average water temperature, and a comparison of the rainfall levels. METHODS: A sample of 3,073 untreated and treated (chlorinated water from taps (1,594, reservoir used to store treated water (1,033, spring water (96 and private well (350 collected for routine testing between 1996 and 1999 was analyzed by the multiple dilution tube methods used to detect the most probable number of total and fecal coliforms. These samples were obtained in the region of Maringá, state of Paraná, Brazil. RESULTS: The highest numbers water samples contaminated by TC (83% and FC (48% were found in the untreated water. TC and FC in samples taken from reservoirs used to store treated water was higher than that from taps midway along distribution lines. Among the treated water samples examined, coliform bacteria were found in 171 of the 1,033 sampling reservoirs. CONCLUSIONS: Insufficient treatment or regrowth is suggested by the observation that more than 17% of these treated potable water contained coliform. TC and FC positive samples appear to be similar and seasonally influenced in treated water. Two different periods must be considered for the occurrence of both TC and FC positive samples: (i a warm-weather period (September-March with high percentage of contaminated samples; and (ii cold-weather period (April-August were they are lower. Both TC and TF positive samples declined with the decreased of water temperature.OBJETIVO: Avaliar a qualidade microbiológica de amostras de água tratada e não-tratada proveniente de comunidades urbanas e rurais e examinar a relação entre ocorrência de coliformes e a média de temperatura da água, e uma comparação dos níveis de precipitação de chuva. MÉTODOS: No período de 1996 a 1999, foram analisadas 3.073 amostras de água

  14. Uso do rejeito da dessalinização de água salobra no cultivo da alface (Lactuca sativa L. em sistema hidropônico NFT Use of the desalted wastewater for lettuce (Lactuca sativa L. production in NFT hydroponic system

    Directory of Open Access Journals (Sweden)

    Rafaelly Suzanye da Silva Santos

    2010-08-01

    Full Text Available A dessalinização por osmose reversa tem sido bastante utilizada para o tratamento de água salobra, possibilitando a sua utilização para o consumo humano, especialmente em regiões semiáridas. Esse processo tem um fator limitante que é a produção de um rejeito de água com alta salinidade. Com o objetivo de avaliar os efeitos da utilização de água de rejeito da dessalinização no cultivo de alface cultivar Vera (Lactuca sativa L. em sistema hidropônico NFT foi conduzido um experimento em casa de vegetação no departamento de ciências ambientais da UFERSA, em delineamento em blocos casualizados, com seis tratamentos e quatro repetições. Os níveis de salinidade da água entre os tratamentos foram obtidos com, ou sem a necessidade de diluição da água de rejeito da dessalinização acrescido da solução nutritiva com salinidade de 1 dS m-1 (100% de água potável de abastecimento - 1,4 dS m-1; 85% abastecimento e 15% água de rejeito - 1,9 dS m-1; 75 % água de rejeito + 25 % água de abastecimento - 2,7 dS m-1; 50% água de rejeito e 50% água de abastecimento - 3,8 dS m-1; 25 % água de rejeito e 75 % água de abastecimento - 4,9 dS m-1 e água de rejeito coletada no dessalinizador - 5,5 dS m-1. Os parâmetros analisados foram área foliar, diâmetro do caule, número de folhas, peso de matéria fresca e o peso de matéria seca. Todas as variáveis estudadas foram influenciadas negativamente de forma linear pelo efeito da concentração de sais na água de irrigação, sendo que a área foliar e a matéria fresca da parte aérea são as que melhor representam a sensibilidade ao estresse salino na cultivar estudada, tendo a produção em termos de matéria fresca uma redução de 94,83%, ao se elevar a CEa de 1,4 a 5,5 dS m-1.The desalination process by reverse osmosis was used for the treatment of brackish water, making possible the usage human consume in semiarid zones. This process has a limiting factor: it produces

  15. Effects of Radiation and Dietary Iron on Expression of Genes and Proteins Involved in Drug Metabolism

    Science.gov (United States)

    Faust, K. M.; Wotring, V. E.

    2014-01-01

    + rad group. Metallothionein is involved in the regulation of physiological metals and also has antioxidant activities. Among the drug metabolism genes examined, ATP binding cassette subfamily B (Abcb1b) gene expression increased more than 10-fold in both groups that received radiation treatments. This increased expression was also seen at the protein level. This ABC transporter carries many different compounds across cell membranes, including administered medications. The cytochrome P450 2E1 enzyme, a mixed-function oxidase that deactivates some medications and activates others, showed about a 2-fold increase in gene expression in both radiation-treated groups, with a trend toward increased expression at the protein level. Expression of epoxide hydrolase, which detoxifies polycyclic aromatic hydrocarbons, showed similar 2-fold increases. Among the DNA repair genes examined, expression of RAD51 was significantly down regulated (1.5 fold) in the radiation treated group. RAD51 is involved in repair of double-stranded DNA breaks. CONCLUSION This experiment used 2 different sources of physiological oxidative stress, administered separately and together, and examined their impacts on liver gene and protein expression. It is clear that significant changes occurred in expression of several genes and proteins in the radiation-treated animals. If the results from this ground analog of portions of the spaceflight environment hold true for the spaceflight environment itself, the physiological roles of the affected enzymes (drug transport and metabolism, redox homeostasis) could mean consequences in redox homeostasis or the pharmacokinetics of administered medications

  16. Presença da toxina microcistina em água, impactos na saúde pública e medidas de controle

    Directory of Open Access Journals (Sweden)

    Sergio Marcos Sanches

    2012-04-01

    Full Text Available As cianobactérias constituem um grupo de microorganismos procariontes encontrado em ambientes aquáticos, os quais vêm sendo pesquisado devido à capacidade de produzir toxinas que causam grandes impactos à saúde pública e ambiental. A ocorrência de florações desses micro-organismos em reservatórios de água utilizada para abastecimento público tem sido frequente e prejudicado os usos múltiplos da água. Algumas cepas produzem florações nocivas liberando compostos neurotóxicos, hepatotóxicos ou irritantes à pele de seres humanos e animais, podendo ocasionar intoxicação e morte. A literatura reporta a ocorrência de um acidente em Caruaru, Pernambuco, onde faleceram 65 pacientes que faziam hemodiálise, devido à presença de microcistina-LR na água que consumiram. Esse estudo objetivou realizar uma revisão sobre a presença da toxina microcistina em água, os impactos e medidas de controle. Foram relatados os principais métodos utilizados para a remoção e quantificação da microcistina em água, bem como sua relação com a saúde humana.

  17. Análise microbiana e do teor de flúor adicionado à água de abastecimento em um estado do Nordeste do Brasil

    OpenAIRE

    Guacyra Machado Lisboa; Taynah Rabelo; Alcides José Ramos Sales; Edna Maria da Silveira Monteiro; Eurípedes Alves da Silva Filho

    2015-01-01

    Objetivo: Analisar os teores de íons de fluoreto e o padrão microbiológico encontrado nas águas de abastecimento público dos municípios alagoanos que fluoretam suas águas. Métodos: Estudo transversal descritivo realizado no período de 2012 a 2014, no qual se analisaram todos os laudos emitidos pelo Laboratório Central de Saúde Pública de Alagoas (LACEN-AL) com os resultados da análise da qualidade da água para consumo humano dos onze municípios alagoanos que fluoretaram suas águas, totalizand...

  18. Avaliação do consumo de água em torneiras em ambiente universitário

    Directory of Open Access Journals (Sweden)

    Antônio Carlos Demanboro

    Full Text Available organizações ao redor do mundo. Além dos benefícios ambientais atualmente, o consumo de água está na agenda da maioria das advindos do uso dos recursos naturais, há também motivações financeiras para continuamente reduzir os custos operacionais. Neste artigo são apresentados os resultados das medições feitas em torneiras na Pontifícia Universidade Católica de Campinas. As medições foram obtidas automaticamente através de uma rede de sensores sem fio, mediante o uso de dispositivos Rfbee e Xbee, especificamente desenvolvidos para coletar os dados. Os principais resultados mostram que o consumo de água é alto e que medidas devem ser tomadas para reduzir o consumo de água para menos de 5 litros por minuto.

  19. Monitoramento sanitário de um sistema integrado de tratamento de águas residuárias da suinocultura

    Directory of Open Access Journals (Sweden)

    Ludmilla S. S. Barros

    2003-12-01

    Full Text Available OBJETIVO: Avaliar o potencial de contaminação de águas residuárias da suinocultura. MÉTODOS: As águas residuárias da suinocultura foram estocadas em um tanque. Depois de 0, 30, 60, 90 e 120 dias de retenção hidráulica, foram incorporadas em lisímetros preenchidos com solos argilosos, arenosos e médios. Esses lisímetros foram então submetidos à simulação de duas condições climáticas: estação de chuvas e estiagem. O número de unidades formadoras de colônias de coliformes totais, coliformes fecais e estreptococos fecais foi medido nos efluentes do tanque de estocagem (para os diversos períodos de retenção hidráulica, nos percolados dos lisímetros e nos três tipos de solo. As análises microbiológicas foram realizadas através da técnica de membrana filtrante. As análises de pH foram realizadas potenciometricamente. RESULTADOS: Para os três microrganismos, as reduções bacterianas máximas no tanque de estocagem foram obtidas a partir de 90 dias de retenção. A quantificação bacteriana nos percolados dos três solos foi caracterizada por reduções elevadas. Em relação aos três tipos de solo, os maiores valores de redução bacteriana foram verificados nos solos médios, devido à sua acidez (pH < 7,0. Para os coliformes fecais a retenção hidráulica não foi suficiente para permitir a adequação sanitária das águas residuárias e sua utilização na irrigação (valores acima de 1 000 UFC.100 mL-1. Nesse caso, a incorporação desses resíduos nos solos foi caracterizada como uma segunda etapa de tratamento. CONCLUSÕES: A retenção da água residuária, seguida da aplicação no solo, mostrou-se eficiente na minimização do efeito contaminante dos resíduos da suinocultura. Recomenda-se uma diminuição no tempo de estocagem das águas residuárias da suinocultura, de 120 para 90 dias.

  20. Cloning of 1,2-Dichloroethane Degradation Genes of Xanthobacter autotrophicus GJ10 and Expression and Sequencing of the dhlA Gene

    NARCIS (Netherlands)

    Janssen, Dick B.; Pries, Frens; Ploeg, Jan van der; Kazemier, Bert; Terpstra, Peter; Witholt, Bernard

    1989-01-01

    A gene bank from the chlorinated hydrocarbon-degrading bacterium Xanthobacter autotrophicus GJ10 was prepared in the broad-host-range cosmid vector pLAFR1. By using mutants impaired in dichloroethane utilization and strains lacking dehalogenase activities, several genes involved in

  1. An Evolutionary Genomic Approach to Identify Genes Involved in Human Birth Timing

    Science.gov (United States)

    Orabona, Guilherme; Morgan, Thomas; Haataja, Ritva; Hallman, Mikko; Puttonen, Hilkka; Menon, Ramkumar; Kuczynski, Edward; Norwitz, Errol; Snegovskikh, Victoria; Palotie, Aarno; Fellman, Vineta; DeFranco, Emily A.; Chaudhari, Bimal P.; McGregor, Tracy L.; McElroy, Jude J.; Oetjens, Matthew T.; Teramo, Kari; Borecki, Ingrid; Fay, Justin; Muglia, Louis

    2011-01-01

    Coordination of fetal maturation with birth timing is essential for mammalian reproduction. In humans, preterm birth is a disorder of profound global health significance. The signals initiating parturition in humans have remained elusive, due to divergence in physiological mechanisms between humans and model organisms typically studied. Because of relatively large human head size and narrow birth canal cross-sectional area compared to other primates, we hypothesized that genes involved in parturition would display accelerated evolution along the human and/or higher primate phylogenetic lineages to decrease the length of gestation and promote delivery of a smaller fetus that transits the birth canal more readily. Further, we tested whether current variation in such accelerated genes contributes to preterm birth risk. Evidence from allometric scaling of gestational age suggests human gestation has been shortened relative to other primates. Consistent with our hypothesis, many genes involved in reproduction show human acceleration in their coding or adjacent noncoding regions. We screened >8,400 SNPs in 150 human accelerated genes in 165 Finnish preterm and 163 control mothers for association with preterm birth. In this cohort, the most significant association was in FSHR, and 8 of the 10 most significant SNPs were in this gene. Further evidence for association of a linkage disequilibrium block of SNPs in FSHR, rs11686474, rs11680730, rs12473870, and rs1247381 was found in African Americans. By considering human acceleration, we identified a novel gene that may be associated with preterm birth, FSHR. We anticipate other human accelerated genes will similarly be associated with preterm birth risk and elucidate essential pathways for human parturition. PMID:21533219

  2. Characterization of differentially expressed genes involved in pathways associated with gastric cancer.

    Directory of Open Access Journals (Sweden)

    Hao Li

    Full Text Available To explore the patterns of gene expression in gastric cancer, a total of 26 paired gastric cancer and noncancerous tissues from patients were enrolled for gene expression microarray analyses. Limma methods were applied to analyze the data, and genes were considered to be significantly differentially expressed if the False Discovery Rate (FDR value was 2. Subsequently, Gene Ontology (GO categories were used to analyze the main functions of the differentially expressed genes. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG database, we found pathways significantly associated with the differential genes. Gene-Act network and co-expression network were built respectively based on the relationships among the genes, proteins and compounds in the database. 2371 mRNAs and 350 lncRNAs considered as significantly differentially expressed genes were selected for the further analysis. The GO categories, pathway analyses and the Gene-Act network showed a consistent result that up-regulated genes were responsible for tumorigenesis, migration, angiogenesis and microenvironment formation, while down-regulated genes were involved in metabolism. These results of this study provide some novel findings on coding RNAs, lncRNAs, pathways and the co-expression network in gastric cancer which will be useful to guide further investigation and target therapy for this disease.

  3. PROSPECÇÃO TECNOLÓGICA DO USO DA PRATA CONTRA MICROORGANISMOS NOCIVOS EM SISTEMAS DE ÁGUA

    Directory of Open Access Journals (Sweden)

    Lucindo José Quintans Júnior

    2011-08-01

    Full Text Available A água, apesar de hoje ser um elemento indispensável à vida, é também uma fonte de contaminação e veículo de transmissão de doenças. As pesquisas e tecnologias realizadas para tratamento de água são inúmeras e tornam-se uma preocupação constante dos pesquisadores. Nesse contexto, um elemento estudado e com seus efeitos antimicrobianos bem conhecidos é a prata (Ag. Assim, esse estudo objetivou a realização de uma prospecção tecnológica nas bases patentearias nacional e mundial, a fim de caracterizar as pesquisas que estão sendo realizadas com o uso da prata nesse meio. O estudo mostrou um acentuado avanço tecnológico na área de tratamento de água, sendo os Estados Unidos o país que mais deposita patentes. Entretanto, quando a pesquisa refere-se à tratamento de água com prata, o número de patentes é extremamente reduzido, tornando o seu uso uma lacuna tecnológica a ser explorada.

  4. Avaliação da concentração de flúor em águas minerais comercializadas no Brasil

    Directory of Open Access Journals (Sweden)

    Rita S. Villena

    1996-12-01

    Full Text Available A utilização de flúor sistêmico deve ser controlada em termos de risco/benefício, pois enquanto a subdosagem não trouxer benefício anticárie, a sobredosagem estará associada com fluorose dental. Assim, uma das preocupações é o consumo de águas minerais, o qual tem aumentado nos últimos anos. Deste modo, foi realizado estudo com o objetivo de analisar a concentração de flúor em águas minerais comercializadas no Brasil. Foram analisadas 104 marcas comerciais, de diferentes regiões do País. Utilizou-se eletrodo específico Orion 96-09 e analisador de íons Orion EA 940, previamente calibrados com soluções padrões de flúor. Foram observadas concentrações de flúor variando de 0,0 a 4,4 ppm. Constataram-se: 1 Concentrações significativas de flúor não relatadas pelo produtor; 2 Concentrações de flúor sem significado preventivo anti-cárie, embora o produtor destaque na embalagem "Água Mineral Fluoretada"; 3 Concentrações de flúor capazes de provocar fluorose dental severa, embora o produtor não faça nenhuma referência que o mesmo existe. Conclui-se ser necessário um Sistema de Vigilância Sanitária para o controle de flúor nas águas minerais oferecidas à população brasileira, quer seja em termos de benefício como de risco.

  5. Hereditary Ovarian Cancer: Not Only BRCA 1 and 2 Genes

    Directory of Open Access Journals (Sweden)

    Angela Toss

    2015-01-01

    Full Text Available More than one-fifth of ovarian tumors have hereditary susceptibility and, in about 65–85% of these cases, the genetic abnormality is a germline mutation in BRCA genes. Nevertheless, several other suppressor genes and oncogenes have been associated with hereditary ovarian cancers, including the mismatch repair (MMR genes in Lynch syndrome, the tumor suppressor gene, TP53, in the Li-Fraumeni syndrome, and several other genes involved in the double-strand breaks repair system, such as CHEK2, RAD51, BRIP1, and PALB2. The study of genetic discriminators and deregulated pathways involved in hereditary ovarian syndromes is relevant for the future development of molecular diagnostic strategies and targeted therapeutic approaches. The recent development and implementation of next-generation sequencing technologies have provided the opportunity to simultaneously analyze multiple cancer susceptibility genes, reduce the delay and costs, and optimize the molecular diagnosis of hereditary tumors. Particularly, the identification of mutations in ovarian cancer susceptibility genes in healthy women may result in a more personalized cancer risk management with tailored clinical and radiological surveillance, chemopreventive approaches, and/or prophylactic surgeries. On the other hand, for ovarian cancer patients, the identification of mutations may provide potential targets for biologic agents and guide treatment decision-making.

  6. Control of energy balance by hypothalamic gene circuitry involving two nuclear receptors, neuron-derived orphan receptor 1 and glucocorticoid receptor.

    Science.gov (United States)

    Kim, Sun-Gyun; Lee, Bora; Kim, Dae-Hwan; Kim, Juhee; Lee, Seunghee; Lee, Soo-Kyung; Lee, Jae W

    2013-10-01

    Nuclear receptors (NRs) regulate diverse physiological processes, including the central nervous system control of energy balance. However, the molecular mechanisms for the central actions of NRs in energy balance remain relatively poorly defined. Here we report a hypothalamic gene network involving two NRs, neuron-derived orphan receptor 1 (NOR1) and glucocorticoid receptor (GR), which directs the regulated expression of orexigenic neuropeptides agouti-related peptide (AgRP) and neuropeptide Y (NPY) in response to peripheral signals. Our results suggest that the anorexigenic signal leptin induces NOR1 expression likely via the transcription factor cyclic AMP response element-binding protein (CREB), while the orexigenic signal glucocorticoid mobilizes GR to inhibit NOR1 expression by antagonizing the action of CREB. Also, NOR1 suppresses glucocorticoid-dependent expression of AgRP and NPY. Consistently, relative to wild-type mice, NOR1-null mice showed significantly higher levels of AgRP and NPY and were less responsive to leptin in decreasing the expression of AgRP and NPY. These results identify mutual antagonism between NOR1 and GR to be a key rheostat for peripheral metabolic signals to centrally control energy balance.

  7. FoxO1 and HNF-4 Are Involved in Regulation of Hepatic Glucokinase Gene Expression by Resveratrol*

    Science.gov (United States)

    Ganjam, Goutham Kumar; Dimova, Elitsa Y.; Unterman, Terry G.; Kietzmann, Thomas

    2009-01-01

    Resveratrol, a polyphenol derived from grapes, exerts important effects on glucose and lipid metabolism, yet detailed mechanisms mediating these effects remain unknown. The liver plays a central role in energy homeostasis, and glucokinase (GK) is a key enzyme involved in glucose utilization. Resveratrol activates SIRT1 (sirtuin 1), which promotes deacetylation of the forkhead transcription factor FoxO1. Previously, we reported that FoxO1 can suppress and that HNF-4 can stimulate GK expression in the liver. Here, we examined the role of FoxO1 and HNF-4 in mediating resveratrol effects on liver GK expression. Resveratrol suppressed hepatic GK expression in vivo and in isolated hepatocytes, and knocking down FoxO1 with shRNAs disrupted this effect. Reporter gene, gel shift, supershift assay, and chromatin immunoprecipitation studies show that FoxO1 binds to the GK promoter and that the interplay between FoxO1 and HNF-4 within the GK promoter is essential for mediating the effects of resveratrol. Resveratrol promotes deacetylation of FoxO1 and enhances its recruitment to the FoxO-binding element. Conversely, resveratrol suppresses recruitment of HNF-4 to its binding site, and knockdown of FoxO1 blocks this effect of resveratrol. Coprecipitation and chromatin immunoprecipitation studies show that resveratrol enhances interaction between FoxO1 and HNF-4, reduces binding of HNF-4 to its own site, and promotes its recruitment to the FoxO site in a FoxO1-dependent manner. These results provide the first evidence that resveratrol represses GK expression via FoxO1 and that the interaction between FoxO1 and HNF-4 contributes to these effects of resveratrol. PMID:19740748

  8. Potencial da água na folha como um indicador de déficit hídrico em milho Leaf water potential as an indicator of water deficit in maize

    Directory of Open Access Journals (Sweden)

    JOÃO ITO BERGONCI

    2000-08-01

    Full Text Available Este trabalho foi desenvolvido na Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul, localizada no município de Eldorado do Sul, nos anos agrícolas de 1993/94 e 1994/95. O objetivo foi avaliar o potencial da água na folha como indicador do déficit hídrico, em milho (Zea mays L., relacionando-o ao potencial da água no solo. O experimento constou de três níveis de irrigação, desde a capacidade de campo até a ausência de irrigação. Os valores do potencial mínimo da água na folha foram desde -1,2 a -1,5 MPa em plantas irrigadas (na capacidade de campo e de -1,6 a -2,0 MPa em plantas não irrigadas. O potencial mínimo da água na folha correlacionou-se com o potencial matricial da água no solo a 45 cm de profundidade (r² = 0,73, e mostrou ser um indicador adequado de déficit hídrico. O potencial da água na folha ao entardecer mostrou relação com o potencial mínimo da água na folha, indicando, assim, que pode ser utilizado como indicador de déficit hídrico. O potencial foliar de base apresentou diferenças evidentes entre os tratamentos extremos, mas não teve relação consistente com o potencial mínimo da água na folha.This study was carried out at the Agronomic Experimental Station of the Federal University of Rio Grande do Sul, in Eldorado do Sul, RS, Brazil, during the agricultural seasons of 1993/94 and 1994/95. The objective was to evaluate the leaf water potential as an indicator of the water deficit in maize (Zea mays L., and its relation with the soil water potential. The experiment comprised three levels of irrigation, from field capacity to absence of irrigation. The values of the minimum leaf water potential ranged from -1.2 to -1.5 MPa in irrigated plants (field capacity and from -1.6 to -2.0 MPa in nonirrigated plants. The minimum leaf water potential was well correlated to the matric water potential measured at 45 cm deep (r² = 0.73. The sunset leaf water potential showed

  9. Overexpression of transcription factor Sp1 leads to gene expression perturbations and cell cycle inhibition.

    Directory of Open Access Journals (Sweden)

    Emmanuelle Deniaud

    Full Text Available BACKGROUND: The ubiquitous transcription factor Sp1 regulates the expression of a vast number of genes involved in many cellular functions ranging from differentiation to proliferation and apoptosis. Sp1 expression levels show a dramatic increase during transformation and this could play a critical role for tumour development or maintenance. Although Sp1 deregulation might be beneficial for tumour cells, its overexpression induces apoptosis of untransformed cells. Here we further characterised the functional and transcriptional responses of untransformed cells following Sp1 overexpression. METHODOLOGY AND PRINCIPAL FINDINGS: We made use of wild-type and DNA-binding-deficient Sp1 to demonstrate that the induction of apoptosis by Sp1 is dependent on its capacity to bind DNA. Genome-wide expression profiling identified genes involved in cancer, cell death and cell cycle as being enriched among differentially expressed genes following Sp1 overexpression. In silico search to determine the presence of Sp1 binding sites in the promoter region of modulated genes was conducted. Genes that contained Sp1 binding sites in their promoters were enriched among down-regulated genes. The endogenous sp1 gene is one of the most down-regulated suggesting a negative feedback loop induced by overexpressed Sp1. In contrast, genes containing Sp1 binding sites in their promoters were not enriched among up-regulated genes. These results suggest that the transcriptional response involves both direct Sp1-driven transcription and indirect mechanisms. Finally, we show that Sp1 overexpression led to a modified expression of G1/S transition regulatory genes such as the down-regulation of cyclin D2 and the up-regulation of cyclin G2 and cdkn2c/p18 expression. The biological significance of these modifications was confirmed by showing that the cells accumulated in the G1 phase of the cell cycle before the onset of apoptosis. CONCLUSION: This study shows that the binding to DNA

  10. Diversification of the insulin-like growth factor 1 gene in mammals.

    Directory of Open Access Journals (Sweden)

    Peter Rotwein

    Full Text Available Insulin-like growth factor 1 (IGF1, a small, secreted peptide growth factor, is involved in a variety of physiological and patho-physiological processes, including somatic growth, tissue repair, and metabolism of carbohydrates, proteins, and lipids. IGF1 gene expression appears to be controlled by several different signaling cascades in the few species in which it has been evaluated, with growth hormone playing a major role by activating a pathway involving the Stat5b transcription factor. Here, genes encoding IGF1 have been evaluated in 25 different mammalian species representing 15 different orders and ranging over ~180 million years of evolutionary diversification. Parts of the IGF1 gene have been fairly well conserved. Like rat Igf1 and human IGF1, 21 of 23 other genes are composed of 6 exons and 5 introns, and all 23 also contain recognizable tandem promoters, each with a unique leader exon. Exon and intron lengths are similar in most species, and DNA sequence conservation is moderately high in orthologous exons and proximal promoter regions. In contrast, putative growth hormone-activated Stat5b-binding enhancers found in analogous locations in rodent Igf1 and in human IGF1 loci, have undergone substantial variation in other mammals, and a processed retro-transposed IGF1 pseudogene is found in the sloth locus, but not in other mammalian genomes. Taken together, the fairly high level of organizational and nucleotide sequence similarity in the IGF1 gene among these 25 species supports the contention that some common regulatory pathways had existed prior to the beginning of mammalian speciation.

  11. Indução da ovulação com gonadotrofina coriônica humana em éguas Crioulas

    Directory of Open Access Journals (Sweden)

    Jordana Beal

    2011-08-01

    Full Text Available O efeito da idade, diâmetro folicular e mês da estação de monta (setembro a janeiro na indução da ovulação com hCG foi avaliado em 123 éguas Crioulas. A idade das éguas variou entre dois e 24 anos e os animais foram examinados diariamente por palpação retal e ultrassonografia com transdutor linear de 5 MHz. Quando os folículos ovarianos atingiram diâmetro de 30 a 35 milímetros aplicou-se uma injeção intravenosa com 1000 UI (n = 39; 1500 UI (n = 41 ou 2000 UI (n = 43 de hCG. As éguas foram cobertas no dia seguinte e examinadas diariamente até a detecção da ovulação. O percentual de éguas que ovularam antes de 24 h da injeção de hCG foi de 10,3%, 7,3% e 4,7%, até 48h após a injeção foi de 92,3%, 85,3% e 86,0%, nos grupos com 1000, 1500 e 2000 UI de hCG, respectivamente. O mês da estação de monta, a idade das éguas ou o diâmetro folicular não influenciaram a resposta ovulatória. As três doses de hCG utilizadas em éguas Crioulas (P >; 0,05 resultaram na indução da ovulação dentro de 48h após a aplicação, quando foi identificado um folículo pré-ovulatório de 30 a 35 mm de diâmetro. Uma única dose de 1000 UI de hCG é eficiente para induzir a ovulação em éguas Crioulas.

  12. Análise da concentração de flúor em águas minerais na região de Araraquara, Brasil

    OpenAIRE

    Brandão Ioneide Maria Gomes; Valsecki Junior Aylton

    1998-01-01

    Está comprovado que indivíduos de todas as idades podem ser beneficiados pelos efeitos tópicos e sistêmicos da fluoretação da água de consumo. Entretanto, o aumento no consumo de água mineral comercial, em substituição ou em adição à água de abastecimento público, pode trazer implicações para a suplementação segura deste íon. Partindo-se disto, em 1995 analisamos a concentração de íon flúor presente em 31 marcas comerciais de água mineral na região de Araraquara, estado de São Paulo, Brasil. ...

  13. Wound healing genes and susceptibility to cutaneous leishmaniasis in Brazil: Role of COL1A1

    Science.gov (United States)

    Almeida, Lucas; Oliveira, Joyce; Guimarães, Luiz Henrique; Carvalho, Edgar M; Blackwell, Jenefer M

    2015-01-01

    Previous studies have demonstrated a role for wound healing genes in resolution of cutaneous lesions caused by Leishmania spp. in both mice and humans, including the gene FLI1 encoding Friend leukaemia virus integration 1. Reduction of Fli1 expression in mice has been shown to result in up-regulation of collagen type I alpha 1 (Col1a1) and alpha 2 (Col1a2) genes and, conversely, in down-regulation of the matrix metalloproteinase 1 (Mmp1) gene, suggesting that Fli1 suppression is involved in activation of the profibrotic gene program. Here we examined single nucleotide polymorphisms (SNPs) in these genes as risk factors for cutaneous (CL) and mucosal leishmaniasis (ML), and leishmaniasis per se, caused by L. braziliensis in humans. SNPs were genotyped in 168 nuclear families (250 CL; 87 ML cases) and replicated in 157 families (402 CL; 39 ML cases). Family-based association tests (FBAT) showed the strongest association between SNPs rs1061237 (combined P=0.002) and rs2586488 (combined P=0.027) at COL1A1 and CL disease. This contributes to our further understanding of the role of wound healing in the resolution of CL disease, providing potential for therapies modulating COL1A1 via drugs acting on FLI1. PMID:25562121

  14. Influência de sais e do pH da água na eficiência de imazethapyr + imazapic no controle de arroz-vermelho Influence of salts and water pH on the efficiency of imazethapyr + imazapic for red rice control

    Directory of Open Access Journals (Sweden)

    D.M. Sanchotene

    2007-01-01

    Full Text Available Na safra agrícola de 2004/2005, em Santa Maria/RS, foi realizado um experimento com o objetivo de avaliar a influência de sais e do pH da água de diferentes fontes de abastecimento de pulverizadores (água de fonte mineral, água de açude e água de poço artesiano sobre a eficiência de imazethapyr (75 g L-1 + imazapic (25 g L-1 no controle de arroz-vermelho (Oryza sativa. No preparo da calda para pulverização do herbicida, foi utilizada água na sua condição original e água acidificada com ácido cítrico até pH 4,5. Foi verificado que os tratamentos com água de pH 4,5 proporcionaram maior eficiência da mistura (imazethapyr + imazapic no controle do arroz-vermelho do que com águas alcalinas (pH 9,4 e 8,7. Todos os tratamentos herbicidas causaram intoxicação às plantas do arroz cultivado. Entretanto, nos tratamentos em que se utilizou água alcalina, foi observada recuperação mais rápida das plantas de arroz, cultivar IRGA 422 CL.This research was carried out in Santa Maria/RS during the 2004/2005 crop season to evaluate the influence of salts and water pH on different sources of spray suppliers (mineral water, dam water and artesian well water on imazethapyr (75 g L-1 + imazapic (25 g L-1 efficiency in red rice control. Thus, water under original condition and acidified water with citric acid to pH 4,5 have been used to prepare the herbicide solutions. It has been verified that the treated plots with water at pH 4,5 showed better action of imazethapyr (75 g L-1 + imazapic (25 g L-1 in the red rice control than alkaline water with pH 9,4 and 8,7. All the treatments with herbicide presented intoxication in the rice. Nevertheless, the treatments with alkaline water showed a fast recuperation in rice plants (IRGA 422CL.

  15. Manejo de água para estabelecimento de alfafa (Medicago sativa L. Water management for alfalfa (Medicago sativa L. establishment

    Directory of Open Access Journals (Sweden)

    Joaquim Bartolomeu Rassini

    2001-04-01

    Full Text Available O manejo de água para o desenvolvimento da alfafa cv. Crioula foi avaliado a campo, com a finalidade de verificar seus efeitos no estabelecimento da planta, até o 1º corte, em 1997. Para isso, verificou-se o comportamento em três estádios de desenvolvimento (E1 = início do estádio vegetativo, E2 = vegetativo pleno e E3 = reprodutivo até colheita em quatro níveis de água (A = 100%, B = 80%, C = 45-50% e D = 20-25% de uma lâmina de irrigação por aspersão. Foram avaliados oito experimentos: 111, 110, 100, 101, 001, 011, 010, 000, em que o primeiro dígito se refere a E1; o segundo, a E2; e o terceiro, a E3. O valor 0 representa a época em que o estádio não recebeu água, coberto por uma estrututura móvel, que deslizava sobre trilhos, e também protegia contra precipitações pluviais casuais. O valor 1 refere-se à época em que houve aplicação dos níveis de água de irrigação. Os estádios de desenvolvimento da planta, em termos de rendimento de matéria seca, responderam de maneira diferente às condições hídricas, sendo E2 e E3 (planta estabelecida os mais sensíveis e E1 (início do estádio vegetativo, o menos sensível, no qual a água foi prejudicial ao estabelecimento e rendimento da planta.The water management for growth of Crioula alfalfa cultivar was evaluated in a field study, in order to assess the effects in the plant establishment up to the first cut, at 1977. The study was carried out in 1997. So that, the behaviour at three growth stages (E1 = early vegetative stage, E2 = full vegetative stage, and E3 = reproductive stage until harvest in the presence of four water levels (A = 100%, B = 80%, C = 45-50%, and D = 20-25% from a sprinkler irrigation was studied. Eight combinations were evaluated: 111, 110, 100, 101, 001, 011, 010, 000, where the first digit is E1, the second is E2 and the third is E3. The number 0 in the figures represents the period in which growth stage did not receive water, cover by a

  16. The effects of calcium on the expression of genes involved in ...

    African Journals Online (AJOL)

    Calcium regulation of the genes involved in ethylene biosynthesis and ethylene receptors in flower abscission zones (AZ) of wild-type tomato (Lycopersicon esculentum Mill.) was investigated in this study. Calcium treatment delayed abscission of pedicel explants. However, verapamil (VP, calcium inhibitor) treatments ...

  17. A L-type lectin gene is involved in the response to hormonal treatment and water deficit in Volkamer lemon.

    Science.gov (United States)

    Vieira, Dayse Drielly Sousa Santana; Emiliani, Giovanni; Bartolini, Paola; Podda, Alessandra; Centritto, Mauro; Luro, François; Carratore, Renata Del; Morillon, Raphaël; Gesteira, Abelmon; Maserti, Biancaelena

    2017-11-01

    Combination of biotic and abiotic stress is a major challenge for crop and fruit production. Thus, identification of genes involved in cross-response to abiotic and biotic stress is of great importance for breeding superior genotypes. Lectins are glycan-binding proteins with a functions in the developmental processes as well as in the response to biotic and abiotic stress. In this work, a lectin like gene, namely ClLectin1, was characterized in Volkamer lemon and its expression was studied in plants exposed to either water stress, hormonal elicitors (JA, SA, ABA) or wounding to understand whether this gene may have a function in the response to multiple stress combination. Results showed that ClLectin1 has 100% homology with a L-type lectin gene from C. sinensis and the in silico study of the 5'UTR region showed the presence of cis-responsive elements to SA, DRE2 and ABA. ClLectin1 was rapidly induced by hormonal treatments and wounding, at local and systemic levels, suggesting an involvement in defence signalling pathways and a possible role as fast detection biomarker of biotic stress. On the other hand, the induction of ClLectin1 by water stress pointed out a role of the gene in the response to drought. The simultaneous response of ClLectin1 expression to water stress and SA treatment could be further investigated to assess whether a moderate drought stress may be useful to improve citrus performance by stimulating the SA-dependent response to biotic stress. Copyright © 2017 Elsevier GmbH. All rights reserved.

  18. Respiratory syncytial virus and TNFalpha induction of chemokine gene expression involves differential activation of Rel A and NF-kappaB1

    Directory of Open Access Journals (Sweden)

    Roebuck Kenneth A

    2002-03-01

    Full Text Available Abstract Background Respiratory syncytial virus (RSV infection of airway epithelial cells stimulates the expression and secretion of a variety of cytokines including the chemotactic cytokines interleukin-8 (IL-8, monocyte chemoattractant protein-1 (MCP-1, and RANTES (regulated upon activation, normal T cell expressed and secreted. Chemokines are important chemoattractants for the recruitment of distinct sets of leukocytes to airway sites of inflammation. Results We have shown previously that chemokine expression is regulated in airway epithelial cells (A549 in a stimulus-specific manner in part through the redox-responsive transcription factors AP-1 and NF-κB. In this study, we examined the NF-κB-mediated effects of RSV and the proinflammatory cytokine TNFα on the induction of IL-8, MCP-1 and RANTES chemokine gene expression in A549 epithelial cells. The results demonstrate that RSV induces chemokine expression with distinct kinetics that is associated with a specific pattern of NF-κB binding activity. This distinction was further demonstrated by the differential effects of the NF-κB inhibitors dexamethasone (DEX and N-acetyl-L-cysteine (NAC. NAC preferentially inhibited RSV induced chemokine expression, whereas DEX preferentially inhibited TNFα induced chemokine expression. DNA binding studies using NF-κB subunit specific binding ELISA demonstrated that RSV and TNFα induced different NF-κB binding complexes containing Rel A (p65 and NF-κB1 (p50. Both TNFα and RSV strongly induced Rel A the activation subunit of NF-κB, whereas only TNFα was able to substantially induce the p50 subunit. Consistent with the expression studies, RSV but not TNFα induction of Rel A and p50 were markedly inhibited by NAC, providing a mechanism by which TNFα and RSV can differentially activate chemokine gene expression via NF-κB. Conclusions These data suggest that RSV induction of chemokine gene expression, in contrast to TNFα, involves redox

  19. DNA glycosylases involved in base excision repair may be associated with cancer risk in BRCA1 and BRCA2 mutation carriers.

    Directory of Open Access Journals (Sweden)

    Ana Osorio

    2014-04-01

    Full Text Available Single Nucleotide Polymorphisms (SNPs in genes involved in the DNA Base Excision Repair (BER pathway could be associated with cancer risk in carriers of mutations in the high-penetrance susceptibility genes BRCA1 and BRCA2, given the relation of synthetic lethality that exists between one of the components of the BER pathway, PARP1 (poly ADP ribose polymerase, and both BRCA1 and BRCA2. In the present study, we have performed a comprehensive analysis of 18 genes involved in BER using a tagging SNP approach in a large series of BRCA1 and BRCA2 mutation carriers. 144 SNPs were analyzed in a two stage study involving 23,463 carriers from the CIMBA consortium (the Consortium of Investigators of Modifiers of BRCA1 and BRCA2. Eleven SNPs showed evidence of association with breast and/or ovarian cancer at p<0.05 in the combined analysis. Four of the five genes for which strongest evidence of association was observed were DNA glycosylases. The strongest evidence was for rs1466785 in the NEIL2 (endonuclease VIII-like 2 gene (HR: 1.09, 95% CI (1.03-1.16, p = 2.7 × 10(-3 for association with breast cancer risk in BRCA2 mutation carriers, and rs2304277 in the OGG1 (8-guanine DNA glycosylase gene, with ovarian cancer risk in BRCA1 mutation carriers (HR: 1.12 95%CI: 1.03-1.21, p = 4.8 × 10(-3. DNA glycosylases involved in the first steps of the BER pathway may be associated with cancer risk in BRCA1/2 mutation carriers and should be more comprehensively studied.

  20. Confirmation of RAX gene involvement in human anophthalmia.

    Science.gov (United States)

    Lequeux, L; Rio, M; Vigouroux, A; Titeux, M; Etchevers, H; Malecaze, F; Chassaing, N; Calvas, P

    2008-10-01

    Microphthalmia and anophthalmia are at the severe end of the spectrum of abnormalities in ocular development. Mutations in several genes have been involved in syndromic and non-syndromic anophthalmia. Previously, RAX recessive mutations were implicated in a single patient with right anophthalmia, left microphthalmia and sclerocornea. In this study, we report the findings of novel compound heterozygous RAX mutations in a child with bilateral anophthalmia. Both mutations are located in exon 3. c.664delT is a frameshifting deletion predicted to introduce a premature stop codon (p.Ser222ArgfsX62), and c.909C>G is a nonsense mutation with similar consequences (p.Tyr303X). This is the second report of a patient with anophthalmia caused by RAX mutations. These findings confirm that RAX plays a major role in the early stages of eye development and is involved in human anophthalmia.

  1. QUALIDADE MICROBIOLÓGICA DA ÁGUA DE CHUVA ARMAZENADA EM CISTERNAS NA ÁREA RURAL DE INHAMBUPE, NO SEMIÁRIDO BAIANO, E SEUS FATORES INTERVENIENTES

    Directory of Open Access Journals (Sweden)

    Nara de Melo Dantas Silva

    2014-09-01

    Full Text Available Com a existência da problemática de carência hídrica no Semiárido Brasileiro devido aos fatores climáticos e edáficos, o sistema de captação e armazenamento de água de chuva, mostra-se como uma solução para o abastecimento de água, melhorando a qualidade de vida dos habitantes das regiões semiáridas, inclusive dos moradores de Inhambupe. Ao estudar os fatores intervenientes da água de chuva armazenada em cisternas da zona rural do município de Inhambupe, pode-se concluir que existe a possibilidade dos microrganismos, bioindicadores e/ou patogênicos, e a matéria orgânica adentra nas cisternas por meio: da ausência do desvio da primeira água, da não existência de calha de proteção das cisternas, da forma inadequada de retirada de água do interior da cisterna, da existência de árvores próximas à superfície de coleta e da criação de animais, como porcos, galinhas e a presença de curral, ocorrendo a entrada de impurezas, estas podem permanecer no interior da cisterna, caso a família não realize sua limpeza periódica e dos seus componentes. Na avaliação da qualidade microbiológica da água de chuva armazenada nas cisternas estudadas, a maioria das amostras analisadas (78,1% apresentaram coliformes termotolerantes. Com relação a presença de bactérias heterotróficas se faz presente em todas as análises de amostras de água de todas as cisternas estudadas. Aplicando-se o planejamento fatorial para identificar os fatores intervenientes da qualidade microbiológica da água das cisternas, tendo como variável resposta ‘coliformes termotolerantes’, e levando-se em consideração a presença dos fatores analisados a um nível de confiança de 10%, os fatores que apresentaram efeitos significativos foram: Balde*Limpeza*Falta Desvio, Falta Desvio, Árvores, a interação dos quatros fatores estudados - Balde*Limpeza*Árvores*Falta Desvio e Árvores*Falta Desvio. Para a variável resposta bactérias heterotr

  2. Genaesthics : Breast Surgery in BRCA1/2 Gene Mutation Carriers

    NARCIS (Netherlands)

    V.M.T. van Verschuer (Victorien)

    2017-01-01

    markdownabstractThe present thesis focuses on breast surgery in BRCA1/2 gene mutation carriers. The topics that are studied vary broadly, representing the multiple disciplines that are involved in the diagnostic work-up and treatment of BRCA1/2-associated breast cancer. The first part contains

  3. Análise microbiológica e bromatológica da água em bebedouros de escolas públicas em Belém do Brejo do Cruz-PB

    Directory of Open Access Journals (Sweden)

    Franklis B. F. Santana

    2015-08-01

    Full Text Available A água é um recurso essencial à sobrevivência de todos os seres vivos e o seu fornecimento em quantidade e qualidade é fundamental para a perfeita manutenção da vida humana. O presente trabalho teve como objetivo realizar a análise microbiológica e bromatológica da água em bebedouros de escolas públicas na cidade de Belém do Brejo do Cruz – PB. Para as análises físico-químicas foram coletadas 100 ml de cada amostra e distribuídas entre as seguintes análises: cor, turbidez e pH, enquanto para a análise microbiológica foi utilizada kit Colilert® (técnica de substrato cromogênio. Foi constatado que para a análise microbiológica as amostra E1, E3, E4 e E5 os resultados foram insatisfatório, pois a portaria vigente estabelece que para a água tratada não se admite a presença de coliformes totais e Escherichia coli em amostra de 100 ml. Para a amostra E2 foi observado resultado satisfatório, fator importante para o ambiente escolar. A amostra E6, não se aplica a análise em virtude da água não ser tratada, ou seja, trata-se de água de poço, caracterizando a mesma como imprópria. Quanto a análise físico-química, de amostras coletadas de bebedouros e caixa d’água pode-se observar que a conclusão dos resultados estão todos satisfatória de acordo com as normas estabelecidas pela Portaria 2.914/11 do Ministério da Saúde. Para que a água chegue às residências em boas condições, é necessário que a Estação de Tratamento da Água mantenha os reservatórios em boas condições para que não ocorram alterações na qualidade da água.

  4. Sistemas de manejo em plantios florestais de eucalipto e perdas de solo e água na região do Vale do Rio Doce, MG

    Directory of Open Access Journals (Sweden)

    Mayesse Aparecida da Silva

    2011-12-01

    Full Text Available A erosão hídrica é a principal responsável pela perda da capacidade produtiva dos solos sob florestas plantadas e alteração da quantidade e qualidade da água em sub-bacias. Assim, este trabalho objetivou avaliar a influência dos sistemas de manejo adotados nos plantios florestais com eucalipto (eucalipto em nível - EN, eucalipto em desnível - ED e eucalipto em desnível com queima - EDQ sobre as perdas de solo e água por erosão hídrica em relação às perdas em floresta nativa (FN, pastagem (PP e solo descoberto (SD, num Latossolo Vermelho (LV e Latossolo Vermelho-Amarelo (LVA, e indicar qual o período mais crítico no manejo do solo, em relação à erosão hídrica, durante o ciclo de cultivo do eucalipto. O estudo foi conduzido em dois municípios: Belo Oriente (LVA e Guanhães (LV, situados no Vale do Rio Doce, região Centro-Leste do estado de Minas Gerais. Com exceção do sistema SD no LVA, as perdas de solo foram abaixo do limite de tolerância admissível para essas classes de solos, nestas regiões, que são de 11,22 Mg ha-1 ano-1 no LV e de 7,17 Mg ha-1 ano-1 para o LVA. As perdas de água nos sistemas com eucalipto foram menores no plantio em nível, no LVA. Nos sistemas com eucalipto, os períodos iniciais após o plantio apresentam as maiores perdas de solo e água com redução ao final do ciclo. Por outro lado, no LVA os sistemas com solo descoberto e floresta nativa apresentaram aumento das perdas de água com o decorrer do tempo avaliado, indicando adequação dos sistemas com eucalipto.

  5. Effect of DGAT1 gene mutation in sows of dam-line on the ...

    African Journals Online (AJOL)

    Diacylglycerol acyltransferase 1 gene (DGAT1) involved in the synthesis and transport of triglycerides is located on chromosome 4 in pigs, in the region with about 200 QTLs responsible among other things for: fat thickness, daily gains, fat content and composition of fatty acids. It is thus probable that the gene polymorphism ...

  6. Vascularização uterina de éguas prenhes de jumento e de garanhão monitoradas por ultrassonografia Doppler

    Directory of Open Access Journals (Sweden)

    Sabina G.C. Lemos

    Full Text Available RESUMO: Este estudo teve como objetivo analisar e descrever os achados ultrassonográficos do útero de éguas gestantes de diferentes idades, de jumento e garanhão, utilizando a técnica de ultrassonografia Doppler Espectral, visando caracterizar os índices de resistência (RI e pulsatilidade (PI até 154 dias de gestação. Foram utilizadas 20 éguas em idade reprodutiva, sem raça definida. As avaliações foram realizadas nas artérias uterinas. Não foi constatada interação entre os valores de RI e PI obtidos nos cornos uterinos (que iniciaram ou não a gestação com o tempo da gestação (P>0,05, desta forma os dados foram discutidos separadamente. Não foram encontradas diferenças entre as avaliações de RI e PI quando comparado os cornos que iniciaram ou não a gestação (P>0,05. Verificou-se efeito de tempo (P0,05. As éguas gestantes de jumento apresentaram maior vascularização uterina no corno não gestante, que as éguas gestantes do garanhão. Com exceção do RI no lado não gravídico, foi verificado efeito de idade da fêmea sobre o lado da gestação (P<0,05, onde as éguas idosas, tanto no corno que iniciou a gestação e o contralateral, tiveram PI e RI mais altos, ou seja, menos vascularizados. Concluiu-se que a vascularização uterina não apresentou diferença entre os cornos uterinos. Há um aumento da vascularização uterina no 70º dia de gestação. Éguas gestantes de jumentos apresentaram maior vascularização uterina, quando comparada às éguas gestantes de garanhões. Éguas com idade acima de 15 anos apresentaram menor vascularização uterina, quando comparadas com idade inferior a 15 anos.

  7. Uso da água por plantas híbridas ou convencionais de arroz irrigado Water use by hybrid and conventional rice plants

    Directory of Open Access Journals (Sweden)

    G. Concenço

    2009-01-01

    Full Text Available Objetivou-se com este trabalho avaliar as características associadas à eficiência do uso da água em plantas de arroz irrigado, híbridas ou convencionais. O experimento foi instalado em casa de vegetação, em delineamento experimental de blocos casualizados em esquema fatorial 2 x 6, com quatro repetições. Os tratamentos constaram de uma planta de arroz da variedade BRS Pelota (convencional ou Inov (híbrida no centro da unidade experimental, que competia com 0, 1, 2, 3, 4 ou 5 plantas da variedade BRS Pelota na periferia da unidade experimental, de acordo com o tratamento. Aos 50 DAE, foram realizadas as avaliações de massa seca da parte aérea, condutância estomática de vapores de água, temperatura da folha e taxa de transpiração, sendo calculada ainda a eficiência do uso da água pela relação entre quantidade de CO2 fixado pela fotossíntese e quantidade de água transpirada. Plantas da variedade híbrida foram superiores ou não diferiram das convencionais quanto à eficiência do uso da água.The objective of this work was to evaluate the characteristics associated to water use efficiency in hybrid or conventional rice plants. The trial was installed under greenhouse conditions, in a completely randomized block design and factorial scheme 2 x 6, with four replications. The treatments consisted of one hybrid (Inov or conventional (BRS Pelota rice plant at the center of the plot surrounded by 0, 1, 2, 3, 4, or 5 conventional (BRS Pelota plants. Fifty days after emergence, shoot dry mass, stomatal conductance of water vapor, leaf temperature and transpiration rates were evaluated, as well as water use efficiency through the relation between the photosynthesis and transpiration rates. Hybrid rice plants were superior to, or did not differ from, the conventional plants in relation to water use efficiency.

  8. Aplicação de boro e reposição de água no solo na cultura do milho forrageiro

    Directory of Open Access Journals (Sweden)

    Márcio José de Santana

    2014-06-01

    Full Text Available Dentre os tratos culturais, a irrigação e a adubação tornam-se imprescindíveis durante a condução da cultura do milho. Dentre os nutrientes o boro exerce um papel importante no crescimento das plantas. Objetivou-se avaliar o desempenho do milho forrageiro (até a fase de corte para volumoso, submetido a diferentes concentrações de boro e níveis de reposição de água. O delineamento experimental foi inteiramente casualizado com 16 tratamentos (4x4, sendo testados quatro reposições de água no solo (70%, 100%, 130% e 160% da evapotranspiração diária e quatro doses de boro (0 mg dm-3; 0,5 mg dm-3; 1,0 mg dm-3 e 1,5 mg dm-3 com quatro repetições. As variáveis avaliadas foram matéria verde e seca aos 35 dias após semeadura (DAS, 55DAS e 90 DAS e a eficiência do uso da água (E.U.A.. Pode-se concluir que a cultura do milho cultivar 30F90 é sensível tanto ao déficit quando ao excesso de água de irrigação; quando irrigou-se com 70% de reposição, houve maior E.U.A.; aos 90 DAS a reposição estimada que proporcionou maior  média de matéria seca foi de 113,6% com um dose de boro ótima de 0,69 mg dm-3.

  9. Daily Rhythms of the Expression of Key Genes Involved in Steroidogenesis and Gonadal Function in Zebrafish.

    Directory of Open Access Journals (Sweden)

    Viviana Di Rosa

    Full Text Available Fish present daily and seasonal rhythms in spawning and plasmatic levels of steroids that control reproduction. However, the existence of the rhythms of expression of the genes that underlie the endocrine mechanisms responsible for processes such as steroidogenesis and reproduction in fish have still been poorly explored to date. Here we investigated the daily pattern of the expression of key genes involved in sex steroid production that ultimately set the sex ratio in fish. Adult zebrafish were maintained under a 12:12 h light-dark cycle at a constant temperature of 27°C and were sampled every 4 h during a 24-hour cycle. The expression of key genes in the gonads and brains of female and male individuals were analyzed. In gonads, the expression of aromatase (cyp19a1a, ovarian aromatase and the antimüllerian hormone (amh, testis was rhythmic, with almost opposite acrophases: ZT 5:13 h (in the light phase and ZT 15:39 h (at night, respectively. The expression of foxl2 (forkhead box L2 was also rhythmic in the ovary (acrophase located at ZT 5:02 h and the expression of dmrt1 (doublesex and mab-3-related transcription factor 1 was rhythmic in testes (acrophase at ZT 18:36 h. In the brain, cyp19a1b (brain aromatase and cyp11b (11beta-hydroxylase presented daily differences, especially in males, where the expression peaked at night. These results provide the first evidence for marked time-of-the-day-dependent differences in the expression of the genes involved in sex ratio control, which should be considered when investigating processes such as reproduction, sex differentiation and steroidogenesis in fish.

  10. Novel mutation in forkhead box G1 (FOXG1) gene in an Indian patient with Rett syndrome.

    Science.gov (United States)

    Das, Dhanjit Kumar; Jadhav, Vaishali; Ghattargi, Vikas C; Udani, Vrajesh

    2014-03-15

    Rett syndrome (RTT) is a severe neurodevelopmental disorder characterized by the progressive loss of intellectual functioning, fine and gross motor skills and communicative abilities, deceleration of head growth, and the development of stereotypic hand movements, occurring after a period of normal development. The classic form of RTT involves mutation in MECP2 while the involvement of CDKL5 and FOXG1 genes has been identified in atypical RTT phenotype. FOXG1 gene encodes for a fork-head box protein G1, a transcription factor acting primarily as transcriptional repressor through DNA binding in the embryonic telencephalon as well as a number of other neurodevelopmental processes. In this report we have described the molecular analysis of FOXG1 gene in Indian patients with Rett syndrome. FOXG1 gene mutation analysis was done in a cohort of 34 MECP2/CDKL5 mutation negative RTT patients. We have identified a novel mutation (p. D263VfsX190) in FOXG1 gene in a patient with congenital variant of Rett syndrome. This mutation resulted into a frameshift, thereby causing an alteration in the reading frames of the entire coding sequence downstream of the mutation. The start position of the frameshift (Asp263) and amino acid towards the carboxyl terminal end of the protein was found to be well conserved across species using multiple sequence alignment. Since the mutation is located at forkhead binding domain, the resultant mutation disrupts the secondary structure of the protein making it non-functional. This is the first report from India showing mutation in FOXG1 gene in Rett syndrome. Copyright © 2014 Elsevier B.V. All rights reserved.

  11. Expression of gentisate 1,2-dioxygenase (gdoA) genes involved in aromatic degradation in two haloarchaeal genera.

    Science.gov (United States)

    Fairley, D J; Wang, G; Rensing, C; Pepper, I L; Larkin, M J

    2006-12-01

    Gentisate-1,2-dioxygenase genes (gdoA), with homology to a number of bacterial dioxygenases, and genes encoding a putative coenzyme A (CoA)-synthetase subunit (acdB) and a CoA-thioesterase (tieA) were identified in two haloarchaeal isolates. In Haloarcula sp. D1, gdoA was expressed during growth on 4-hydroxybenzoate but not benzoate, and acdB and tieA were not expressed during growth on any of the aromatic substrates tested. In contrast, gdoA was expressed in Haloferax sp. D1227 during growth on benzoate, 3-hydroxybenzoate, cinnamate and phenylpropionate, and both acdB and tieA were expressed during growth on benzoate, cinnamate and phenylpropionate, but not on 3-hydroxybenzoate. This pattern of induction is consistent with these genes encoding steps in a CoA-mediated benzoate pathway in this strain.

  12. BIOMETRIA DE MUDAS DE CAJUEIRO ANÃO IRRIGADAS COM ÁGUAS SALINAS E USO DE ATENUADORES DO ESTRESSE SALINO

    Directory of Open Access Journals (Sweden)

    Eliésia Carla de Medeiros Torres

    2014-06-01

    Full Text Available A salinidade hídrica é fator de estresse que altera o crescimento de mudas de frutíferas. Objetivou-se avaliar o crescimento de mudas de cajueiro anão precoce em substratos irrigados com águas salinas e adição de biofertilizante bovino e cobertura com biomassa vegetal no Seridó Paraibano. O experimento foi instalado em delineamento em blocos casualizados, com três repetições, em arranjo fatorial 5 x 2 x 2, correspondente a cinco níveis de salinidade da água de irrigação (0,5; 3,5; 6,5; 9,5 e 12,5 dS m-1, no solo sem e com biofertilizante bovino, numa alíquota de 0,2 dm-3 por planta, sem e com cobertura morta com 3 cm de casca de arroz. As taxas de crescimento absoluto e relativo em altura, em diâmetro caulinar, número de folhas, área foliar, comprimento da raiz e fitomassa fresca do cajueiro anão precoce foram influenciados negativamente pela salinidade da água de irrigação. O biofertilizante mitigou os efeitos depressivos dos sais no crescimento absoluto das plantas irrigadas com água de salinidade até 3,5 dS m-1. O uso simultâneo do biofertilizante e cobertura morta foi eficiente na redução dos efeitos dos sais nas plantas irrigadas com águas de 3,5 e 6,5 dS m-1, promovendo aumento no número de folhas. O biofertilizante incrementou a fitomassa fresca total das plantas irrigadas com água de maior condutividade elétrica.Palavra-chave: agroecologia, biofertilizante, crescimento, fruticultura, salinidade. SEEDLING OF DWARF CASHEW BIOMETRY IRRIGATED WITH SALINE WATER AND SALT STRESS ATTENUATORS USE   ABSTRACTThe water salinity is a stress factor that alters the growth of fruit seedlings. This study aimed to evaluate the growth of dwarf cashew seedlings in substrates irrigated with saline water and bovine bio fertilizer addition and cover with vegetable biomass in Seridó, Paraiba. The experiment was arranged in a randomized block design with three replications in a factorial 5 x 2 x 2, corresponding to five

  13. Remoção de nitrato da água utilizando bagaço de caju quimicamente modificado

    Directory of Open Access Journals (Sweden)

    Raimundo Renato Melo Neto

    2013-09-01

    Full Text Available O ânion nitrato (NO-3, devido sua alta solubilidade em água, é um contaminante de difícil remoção. Os métodos atualmente empregados para tratamento de água contaminada por nitrato possuem um elevado custo e não são muito eficientes, já que em alguns casos são gerados resíduos mais tóxicos que o próprio nitrato. Tecnologias contemporâneas empregadas para tratamento de efluentes aquosos têm seus mecanismos baseados no fenômeno de adsorção, em que um sólido poroso é capaz de adsorver determinadas substâncias, quando estas estão em contato. Estas tecnologias são economicamente rentáveis quando são empregados rejeitos agrícolas como adsorventes. Baseado no exposto este trabalho tem como objetivo avaliar a eficiência de remoção de nitrato de água, utilizando bagaço de caju quimicamente modificado. Os estudos foram realizados em batelada. Os ensaios de adsorção evidenciaram que o tratamento do bagaço de caju com NaOH 0,1 mol.L-1  alcançou uma eficiência de remoção de 96%, enquanto que o bagaço sem tratamento obteve remoção de 22%. O mecanismo de adsorção modelou-se a uma cinética de pseudo-segunda ordem e foi mais bem correlacionado com a isoterma de Freundlich.  

  14. Domestication-driven Gossypium profilin 1 (GhPRF1) gene transduces early flowering phenotype in tobacco by spatial alteration of apical/floral-meristem related gene expression.

    Science.gov (United States)

    Pandey, Dhananjay K; Chaudhary, Bhupendra

    2016-05-13

    Plant profilin genes encode core cell-wall structural proteins and are evidenced for their up-regulation under cotton domestication. Notwithstanding striking discoveries in the genetics of cell-wall organization in plants, little is explicit about the manner in which profilin-mediated molecular interplay and corresponding networks are altered, especially during cellular signalling of apical meristem determinacy and flower development. Here we show that the ectopic expression of GhPRF1 gene in tobacco resulted in the hyperactivation of apical meristem and early flowering phenotype with increased flower number in comparison to the control plants. Spatial expression alteration in CLV1, a key meristem-determinacy gene, is induced by the GhPRF1 overexpression in a WUS-dependent manner and mediates cell signalling to promote flowering. But no such expression alterations are recorded in the GhPRF1-RNAi lines. The GhPRF1 transduces key positive flowering regulator AP1 gene via coordinated expression of FT4, SOC1, FLC1 and FT1 genes involved in the apical-to-floral meristem signalling cascade which is consistent with our in silico profilin interaction data. Remarkably, these positive and negative flowering regulators are spatially controlled by the Actin-Related Protein (ARP) genes, specifically ARP4 and ARP6 in proximate association with profilins. This study provides a novel and systematic link between GhPRF1 gene expression and the flower primordium initiation via up-regulation of the ARP genes, and an insight into the functional characterization of GhPRF1 gene acting upstream to the flowering mechanism. Also, the transgenic plants expressing GhPRF1 gene show an increase in the plant height, internode length, leaf size and plant vigor. Overexpression of GhPRF1 gene induced early and increased flowering in tobacco with enhanced plant vigor. During apical meristem determinacy and flower development, the GhPRF1 gene directly influences key flowering regulators through ARP-genes

  15. A compendium and functional characterization of mammalian genes involved in adaptation to Arctic or Antarctic environments.

    Science.gov (United States)

    Yudin, Nikolay S; Larkin, Denis M; Ignatieva, Elena V

    2017-12-28

    Many mammals are well adapted to surviving in extremely cold environments. These species have likely accumulated genetic changes that help them efficiently cope with low temperatures. It is not known whether the same genes related to cold adaptation in one species would be under selection in another species. The aims of this study therefore were: to create a compendium of mammalian genes related to adaptations to a low temperature environment; to identify genes related to cold tolerance that have been subjected to independent positive selection in several species; to determine promising candidate genes/pathways/organs for further empirical research on cold adaptation in mammals. After a search for publications containing keywords: "whole genome", "transcriptome or exome sequencing data", and "genome-wide genotyping array data" authors looked for information related to genetic signatures ascribable to positive selection in Arctic or Antarctic mammalian species. Publications related to Human, Arctic fox, Yakut horse, Mammoth, Polar bear, and Minke whale were chosen. The compendium of genes that potentially underwent positive selection in >1 of these six species consisted of 416 genes. Twelve of them showed traces of positive selection in three species. Gene ontology term enrichment analysis of 416 genes from the compendium has revealed 13 terms relevant to the scope of this study. We found that enriched terms were relevant to three major groups: terms associated with collagen proteins and the extracellular matrix; terms associated with the anatomy and physiology of cilium; terms associated with docking. We further revealed that genes from compendium were over-represented in the lists of genes expressed in the lung and liver. A compendium combining mammalian genes involved in adaptation to cold environment was designed, based on the intersection of positively selected genes from six Arctic and Antarctic species. The compendium contained 416 genes that have been

  16. Uso de destiladores solares para fornecer água potável no semiárido Paraibano

    Directory of Open Access Journals (Sweden)

    José Adailton Lima Silva

    2016-08-01

    Full Text Available A escassez de água advinda das condições climáticas do semiárido paraibano tem forçado inúmeras famílias rurais a consumirem água de má qualidade, o que tem contribuído para o aumento de casos de doenças transmitidas pela água. Tendo em vista esta problemática, objetivou-se analisar como o uso de destiladores solares pode proporcionar a obtenção de água potável, e avaliar os benefícios socioeconômicos e ambientais advindos do uso desta tecnologia. Após os estudos, observou-se que os destiladores solares são uma tecnologia de baixo custo econômico, de fácil disseminação social, utilizam energia solar (limpa e renovável, e são capazes de fornecer água potável para atender as demandas hídricas de famílias que convivem com a escassez hídrica em regiões semiáridas.Use of solar distillers to provide drinking water in semiarid ParaibanoAbstract: The scarcity of water arising from climatic conditions of semi-arid Paraiba has forced many rural families to consume poor quality water, which has contributed to the increase in cases of waterborne diseases. In view of this problem, the objective was to examine how the use of solar distillers can provide the production of drinking water, and assess the socio-economic and environmental benefits from the use of this technology. After the studies, it was observed that solar distillers are a low economic cost technology, easy social diffusion, uses solar energy (clean, renewable, and are able to provide drinking water to meet the water demands of families living with the shortage of water in semi-arid regions

  17. Gene-gene interaction between MSX1 and TP63 in Asian case-parent trios with nonsyndromic cleft lip with or without cleft palate.

    Science.gov (United States)

    Liu, Dongjing; Schwender, Holger; Wang, Mengying; Wang, Hong; Wang, Ping; Zhu, Hongping; Zhou, Zhibo; Li, Jing; Wu, Tao; Beaty, Terri H

    2018-03-01

    Small ubiquitin-like modification, also known as sumoylation, is a crucial post-translational regulatory mechanisms involved in development of the lip and palate. Recent studies reported two sumoylation target genes, MSX1 and TP63, to have achieved genome-wide level significance in tests of association with nonsyndromic clefts. Here, we performed a candidate gene analysis considering gene-gene and gene-environment interaction for SUMO1, MSX1, and TP63 to further explore the etiology of nonsyndromic cleft lip with or without cleft palate (NSCL/P). A total of 130 single-nucleotide polymorphisms (SNPs) in or near SUMO1, MSX1, and TP63 was analyzed among 1,038 Asian NSCL/P trios ascertained through an international consortium. Conditional logistic regression models were used to explore gene-gene (G × G) and gene-environment (G × E) interaction involving maternal environmental tobacco smoke and multivitamin supplementation. Bonferroni correction was used for G × E analysis and permutation tests were used for G × G analysis. While transmission disequilibrium tests and gene-environment interaction analysis showed no significant results, we did find signals of gene-gene interaction between SNPs near MSX1 and TP63. Three pairwise interactions yielded significant p values in permutation tests (rs884690 and rs9290890 with p = 9.34 × 10 -5 and empirical p = 1.00 × 10 -4 , rs1022136 and rs4687098 with p = 2.41 × 10 -4 and empirical p = 2.95 × 10 -4 , rs6819546 and rs9681004 with p = 5.15 × 10 -4 and empirical p = 3.02 × 10 -4 ). Gene-gene interaction between MSX1 and TP63 may influence the risk of NSCL/P in Asian populations. Our study provided additional understanding of the genetic etiology of NSCL/P and underlined the importance of considering gene-gene interaction in the etiology of this common craniofacial malformation. © 2018 Wiley Periodicals, Inc.

  18. Modelagem da qualidade da água do rio Poti em Teresina (PI

    Directory of Open Access Journals (Sweden)

    Antônio Alves de Oliveira Filho

    2017-07-01

    Full Text Available RESUMO O crescimento desordenado da capital piauiense - marcado, sobretudo, pela ocupação habitacional às margens do rio Poti e pela existência de ligações clandestinas de esgoto bruto nas tubulações de drenagem pluvial - tem contribuído significativamente para a poluição das águas da bacia hidrográfica do rio Parnaíba (região semiárida do Brasil. A presente pesquisa tem como objetivo realizar a modelagem matemática da qualidade da água em um trecho de 36,8 km de extensão do rio Poti, com base na plataforma QUAL-UFMG. A pesquisa apresenta-se como o primeiro estudo envolvendo modelagem da qualidade da água no referido rio. Os componentes modelados foram: oxigênio dissolvido (OD, demanda bioquímica de oxigênio (DBO e coliformes termotolerantes (CT. Os resultados das medições de campo indicaram desconformidades do parâmetro CT com relação à Resolução CONAMA nº 357/2005. A calibração dos coeficientes de decaimento para cada parâmetro resultou em desvios médios entre dados medidos e modelados de até 20% e coeficientes de eficiência de Nash-Sutcliffe superiores a 0,75, o que indica que o QUAL-UFMG pode ser utilizado como base para predição da qualidade da água em rios localizados em regiões semiáridas. O modelo calibrado também foi comparado aos dados de campo obtidos na literatura. Finalmente, foram realizadas simulações do modelo para diferentes cenários de vazão (mínimas e máximas, apresentando resultados coerentes e que podem ser utilizados para a gestão dos recursos hídricos do estado do Piauí.

  19. Indicadores de eficiência do uso da água e de nutrientes de clones de palma forrageira em condições de sequeiro no Semiárido brasileiro

    Directory of Open Access Journals (Sweden)

    Thieres George Freire Silva

    2014-06-01

    Full Text Available Objetivou-se avaliar a eficiência produtiva de três clones de palma forrageira resistentes à Cochonilha do Carmim, cultivados em condições de sequeiro no Semiárido brasileiro. Os clones avaliados foram IPA-Sertânia (IPA, Miúda (MIU e Orelha de Elefante Mexicana (OEM. Foram calculados os indicadores de eficiência do uso da água: produtividade da água da cultura (PA C, produtividade econômica da água (PEA e eficiência do uso de nutrientes (EUN, todos com base na água precipitada (Prec. e na evapotranspiração real da cultura (ETr. A ETr foi quantificada por meio do método do balanço de água no solo. Verificou-se que não houve diferença estatística entre a PA C em base seca (MS nos clones avaliados tanto em termos de Prec. como de ETr, mas eles diferiram em base fresca. A OEM foi o clone mais eficiente em base fresca (PA C(Prec. ~ 104,8 kg MV ha-1 mm-1; PA C(ETr. ~ 112,1 kg MV ha-1 mm-1, seguido pelo clone IPA, que também se mostrou eficiente quanto a ETr (PA C(ETr. ~ 101,1 kg MV ha-1 mm-1. A MIU foi o clone menos eficiente para o Semiárido brasileiro. Não houve diferença da PEA (PEA(Prec. ~ 35,4 R$ ha-1 mm-1; PEA(ETr. ~ 39,5 R$ ha-1 mm-1 e da EUN, com exceção da eficiência do uso do magnésio com base na ETr, que foi maior para OEM (EUN(Mg ~ 111,4 g ha-1 mm-1 e IPA (EUN(Mg ~ 77,4 g ha-1 mm-1, e do sódio com base na precipitação, que foi superior para os clones OEM (EUN(Mg ~ 4854,3 mg ha-1 mm-1 e MIU (EUN(Mg ~ 3383,6 mg ha-1 mm-1.

  20. Mutational analysis of two structural genes of the remperate lactococcal bacteriophage TP901-1 involved in tail length determination and baseplate assembly

    DEFF Research Database (Denmark)

    Pedersen, Margit; Østergaard, Solvej; Bresciani, José

    2000-01-01

    Two putative structural genes, orf tmp (tape measure protein) and orf bpp (baseplate protein), of the temperate lactococcal phage TP901-1 were examined by introduction of specific mutations in the prophage strain Lactococcus lactic ssp. cremoris 901-1. The adsorption efficiencies of the mutated...... or duplication of 29% in orf tmp was shown to shorten or lengthen the phage tail by approximately 30%, respectively. The orf tmp is proposed to function as a tape measure protein, TMP, important for assembly of the TP901-1 phage tail and involved in tail length determination. Specific mutations in orf bpp...... produced phages which were unable to adsorb to the indicator strain and electron microscopy revealed particles lacking the baseplate structure. The orf bpp is proposed to encode a highly immunogenic structural baseplate protein, BPP, important for assembly of the baseplate. Finally, an assembly pathway...

  1. An in silico analysis of the key genes involved in flavonoid biosynthesis in Citrus sinensis

    Directory of Open Access Journals (Sweden)

    Adriano R. Lucheta

    2007-01-01

    Full Text Available Citrus species are known by their high content of phenolic compounds, including a wide range of flavonoids. In plants, these compounds are involved in protection against biotic and abiotic stresses, cell structure, UV protection, attraction of pollinators and seed dispersal. In humans, flavonoid consumption has been related to increasing overall health and fighting some important diseases. The goals of this study were to identify expressed sequence tags (EST in Citrus sinensis (L. Osbeck corresponding to genes involved in general phenylpropanoid biosynthesis and the key genes involved in the main flavonoids pathways (flavanones, flavones, flavonols, leucoanthocyanidins, anthocyanins and isoflavonoids. A thorough analysis of all related putative genes from the Citrus EST (CitEST database revealed several interesting aspects associated to these pathways and brought novel information with promising usefulness for both basic and biotechnological applications.

  2. Low-intensity laser irradiation at 660 nm stimulates transcription of genes involved in the electron transport chain.

    Science.gov (United States)

    Masha, Roland T; Houreld, Nicolette N; Abrahamse, Heidi

    2013-02-01

    Low-intensity laser irradiation (LILI) has been shown to stimulate cellular functions leading to increased adenosine triphosphate (ATP) synthesis. This study was undertaken to evaluate the effect of LILI on genes involved in the mitochondrial electron transport chain (ETC, complexes I-IV) and oxidative phosphorylation (ATP synthase). Four human skin fibroblast cell models were used in this study: normal non-irradiated cells were used as controls while wounded, diabetic wounded, and ischemic cells were irradiated. Cells were irradiated with a 660 nm diode laser with a fluence of 5 J/cm(2) and gene expression determined by quantitative real-time reverse transcription (RT) polymerase chain reaction (PCR). LILI upregulated cytochrome c oxidase subunit VIb polypeptide 2 (COX6B2), cytochrome c oxidase subunit VIc (COX6C), and pyrophosphatase (inorganic) 1 (PPA1) in diabetic wounded cells; COX6C, ATP synthase, H+transporting, mitochondrial Fo complex, subunit B1 (ATP5F1), nicotinamide adenine dinucleotide (NADH) dehydrogenase (ubiquinone) 1 alpha subcomplex, 11 (NDUFA11), and NADH dehydrogenase (ubiquinone) Fe-S protein 7 (NDUFS7) in wounded cells; and ATPase, H+/K+ exchanging, beta polypeptide (ATP4B), and ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C2 (subunit 9) (ATP5G2) in ischemic cells. LILI at 660 nm stimulates the upregulation of genes coding for subunits of enzymes involved in complexes I and IV and ATP synthase.

  3. Possível associação entre tipos de hemoglobina e problemas reprodutivos em éguas Mangalarga brasileiras

    Directory of Open Access Journals (Sweden)

    Rejane de Lima e Silva

    1996-09-01

    Full Text Available Foram estudadas as hemoglobinas de 100 éguas da raça Mangalarga, em idade de reprodução, provenientes da Fazenda Santa Fé, situada na região de Botucatu. Esses animais foram divididos em 2 grupos, de acordo com o histórico reprodutivo de cada animal, sendo um formado por éguas reprodutivamente normais e o segundo por éguas portadoras de problemas reprodutivos. Foram colhidas amostras de 15 ml de sangue com anticoagulante. As hemoglobinas foram identificadas por meio de eletroforese em gel de poliacrilamida em placa vertical, a 7% em pH 8.6, segundo Davis11 (1964. Quanto ao sistema de hemoglobinas, foram encontrados os seguintes fenótipos para o grupo de éguas reprodutivamente normais:A¹--- (2,0%, A¹, A²m+m+ (21,0% e A¹A² m+m (27,0%; para o grupo de éguas com problemas reprodutivos: A¹--- (10,0%, A¹A² m+m+ (12,0% e A¹A²m+m+ (28,0%. A diferença na freqüência do fenótipo A¹--- entre os grupos pode ter ocorrido devido ã existência da ligação do loco hemoglobina a outro que controlaria características de produção. Além disso, pode estar ocorrendo influência do tipo de clima existente nos trópicos.

  4. The landscape of human genes involved in the immune response to parasitic worms

    Directory of Open Access Journals (Sweden)

    Fumagalli Matteo

    2010-08-01

    Full Text Available Abstract Background More than 2 billion individuals worldwide suffer from helminth infections. The highest parasite burdens occur in children and helminth infection during pregnancy is a risk factor for preterm delivery and reduced birth weight. Therefore, helminth infections can be regarded as a strong selective pressure. Results Here we propose that candidate susceptibility genes for parasitic worm infections can be identified by searching for SNPs that display a strong correlation with the diversity of helminth species/genera transmitted in different geographic areas. By a genome-wide search we identified 3478 variants that correlate with helminth diversity. These SNPs map to 810 distinct human genes including loci involved in regulatory T cell function and in macrophage activation, as well as leukocyte integrins and co-inhibitory molecules. Analysis of functional relationships among these genes identified complex interaction networks centred around Th2 cytokines. Finally, several genes carrying candidate targets for helminth-driven selective pressure also harbour susceptibility alleles for asthma/allergy or are involved in airway hyper-responsiveness, therefore expanding the known parallelism between these conditions and parasitic infections. Conclusions Our data provide a landscape of human genes that modulate susceptibility to helminths and indicate parasitic worms as one of the major selective forces in humans.

  5. Evapotranspiração da cultura da melancia irrigada com água de diferentes salinidades Evapotranspiration of watermelon irrigated with different salinity waters

    Directory of Open Access Journals (Sweden)

    Vladimir B. Figueirêdo

    2009-06-01

    Full Text Available Sabe-se que a determinação precisa da evapotranspiração da cultura (ETc é de grande importância para o uso eficiente da água, principalmente em regiões áridas e semiáridas onde se faz necessária a utilização de água salina para irrigação. O objetivo deste trabalho foi determinar a evapotranspiração da melancia, cultivar Mickylee, com o uso de diferentes níveis de salinidade da água de irrigação. O experimento foi conduzido na Fazenda Experimental da Alagoinha, pertencente à Universidade Federal Rural do Semiárido, Mossoró - RN. Os níveis de salinidade da água de irrigação foram: S1 = 0,55; S2 = 1,65; S3 = 2,35; S4 = 3,5, e S5 = 4,5 dS m-1, sendo os tratamentos dispostos no delineamento de blocos ao acaso, com quatro repetições. A determinação da evapotranspiração de referência (ETo foi realizada pelo método FAO-Penmam-Monteith, e a ETc, pelas leituras obtidas em lisímetros de pesagem, instalados nos tratamentos S1 e S5. Os resultados mostraram que a ETc da melancia diminui com o aumento da salinidade da água aplicada e que a evapotranspiração total durante o ciclo foi de 245 e 214 mm, respectivamente, para os tratamentos S1 e S5. Os valores médios de Kc obtidos para cada fase fenológica foram 0,23; 0,68; 1,12; 0,90 e 0,24; 0,61; 0,98 e 0,78, respectivamente, para as águas S1 e S5.The knowledge of crop evapotranspiration (ETc is very important for efficient water use in irrigated crops, mainly in arid and semi-arid regions, where the use of saline water is common. The objective of this study was to determine watermelon crop evapotranspiration (cv. Mickylee in plants submitted to different irrigation water salinity levels. The experiment was carried out at the Experimental field of the Semi-Arid Federal Rural University, Mossoró, RN, Brazil. The irrigation water salinity levels were of S1 = 0.55; S2 = 1.65; S3 = 2.35; S4 = 3.5 and S5 = 4.5 dS m-1, with the treatments set up in randomized blocks with

  6. REGIME TÉRMICO EM ÁGUAS CORRENTES E SUA IMPORTÂNCIA NA ESTRUTURA DO HABITAT E NA BIOLOGIA DE ORGANISMOS AQUÁTICOS

    Directory of Open Access Journals (Sweden)

    Ibraim Fantin-Cruz

    2011-01-01

    Full Text Available O regime térmico em águas correntes e os processos responsáveis por sua variabilidade, são de grande importância ecológica. Uma vez que a temperatura da água influencia a distribuição, reprodução, crescimento e desenvolvimento dos organismos aquáticos, além de produzir efeitos sobre o metabolismo ecossistêmico. Nesta perspectiva, este trabalho revisou os aspectos relevantes à variabilidade térmica de águas correntes, os fatores que os influenciam e sua importância na estrutura do habitat e na biologia de organismos aquáticos. Com este propósito, observou-se que flutuações na temperatura da água podem ocorrer naturalmente devido às condições atmosféricas, topografia, descarga e condições do leito ou por perturbações antrópicas, tais como, represamento, desmatamento e mudanças climáticas. A heterogeneidade térmica ocorre desde escalas locais em gradientes verticais de temperatura da coluna de água, bem como em escalas regionais ao longo do gradiente longitudinal. Flutuações temporais da temperatura também são evidentes e ocorrem diariamente (hora do dia e sazonalmente (estações do ano. Tanto espacial quanto temporalmente, a estabilidade (ou variabilidade da temperatura depende da descarga do rio. A temperatura da água influi diretamente nas condições do habitat provocando alterações nas reações químicas, reduzindo as concentrações de oxigênio na água, formando barreiras térmicas, entre outros efeitos. Tais condições podem causar elevação da taxa de crescimento de plantas aquáticas, desequilíbrio no período de eclosão de ovos (principalmente de peixes, aumento à suscetibilidade a doenças, alterando, desta forma, o funcionamento do ecossistema. Portanto, torna-se evidente a importância da temperatura da água como fator relevante no controle ambiental de águas superficiais.

  7. Association between Age at Diagnosis of Graves' Disease and Variants in Genes Involved in Immune Response

    Science.gov (United States)

    Jurecka-Lubieniecka, Beata; Ploski, Rafal; Kula, Dorota; Krol, Aleksandra; Bednarczuk, Tomasz; Kolosza, Zofia; Tukiendorf, Andrzej; Szpak-Ulczok, Sylwia; Stanjek-Cichoracka, Anita; Polanska, Joanna; Jarzab, Barbara

    2013-01-01

    Background Graves' disease (GD) is a complex disease in which genetic predisposition is modified by environmental factors. The aim of the study was to examine the association between genetic variants in genes encoding proteins involved in immune response and the age at diagnosis of GD. Methods 735 GD patients and 1216 healthy controls from Poland were included into the study. Eight genetic variants in the HLA-DRB1, TNF, CTLA4, CD40, NFKb, PTPN22, IL4 and IL10 genes were genotyped. Patients were stratified by the age at diagnosis of GD and the association with genotype was analysed. Results Polymorphism in the HLA-DRB1, TNF and CTLA4 genes were associated with GD. The carriers of the HLA DRB1*03 allele were more frequent in patients with age at GD diagnosis ≤30 years than in patients with older age at GD diagnosis. Conclusions HLADRB1*03 allele is associated with young age at diagnosis of Graves' disease in polish population. PMID:23544060

  8. O COMPORTAMENTO DO CONSUMIDOR DE ÁGUA MINERAL: UM ESTUDO EXPLORATÓRIO SOBRE AS VARIÁVEIS QUE INFLUENCIAM O CONSUMO.

    OpenAIRE

    Anonymous

    2006-01-01

    A água mineral foi o produto que mais apresentou crescimento em consumo entre as famílias brasileiras nos últimos trinta anos, ultrapassando 0,320 litros per capita/ano em 1974/1975 e alcançando a marca de 18,541 litros em 2002/2003. Dentre os produtos de maior consumo alimentar, somente o leite fica à frente da água mineral. No âmbito mundial, o consumo de água envasada deverá continuar crescendo nos próximos anos, passando de um volume de 155 bilhões de litros em 2003 para 206 bilhões em 20...

  9. AVALIAÇÃO DAS CONCENTRAÇÕES DE NITRATO NAS ÁGUAS MINERAIS PRODUZIDAS NA REGIÃO DA GRANDE NATAL

    OpenAIRE

    Nóbrega, Mariana Magna Santos da; Araújo, André Luis Calado; Santos, Jerônimo Pereira dos

    2009-01-01

    RESUMO A Região Metropolitana de Natal, no estado do Rio Grande do Norte, é detentora de grandes reservas de águas subterrâneas pertencentes ao aqüífero Dunas/Barreiras. Essas águas são utilizadas pela população para seu abastecimento e consumo industrial. Além disso, essas reservas possuem características de águas minerais, localizando-se principalmente nos municípios de Parnamirim e Macaíba na região sul e no município de Extremoz, na parte norte. Em contrapartida, o crescimento urbano acel...

  10. Uso eficiente da água : uma contribuição para o desenvolvimento sustentável da agropecuária

    OpenAIRE

    Faggion, Francisco; Oliveira, Carlos Alberto da Silva; Christofidis, Demétrios

    2009-01-01

    Na maioria das áreas agrícolas irrigadas o volume de água utilizado é superior ao realmente necessário para a produção satisfatória de alimentos. A eficiência no uso da água pode auxiliar na resolução dos problemas de escassez, diminuir conflitos entre usuários e aumentar, estabilizar e garantir a produção. Este trabalho mostra possíveis alternativas para o uso eficiente da água na agropecuária baseado em diretrizes desenvolvidas na Europa e Estados Unidos, procurando estabelecer critérios de...

  11. Unidades de produção agrícola controlada no semiárido para o tratamento de água cinza

    Directory of Open Access Journals (Sweden)

    Aline Costa Ferreira

    2013-10-01

    Full Text Available As reservas de água doce, juntamente com as limitações de lançamento de efluentes no meio ambiente, culminam para a necessidade do uso racional dos recursos hídricos de modo a reduzir os impactos negativos da geração de efluentes. Nesse contexto, o trabalho foi realizado objetivando-se implantar e monitorar eficiência do tratamento da água, para o semiárido, de unidades de produção agrícola controladas UPAC’s utilizando as águas cinzas de uma lavanderia comunitária.  A pesquisa foi desenvolvida nas instalações da Lavanderia Pública do Distrito de Ribeira no município de Cabaceiras no Estado da Paraíba. Foram implantadas oito unidades de produção agrícola controlada e os parâmetros analisados das águas cinza coletadas dos pontos de observação das unidades foram: Condutividade Elétrica (CE, pH, e Oxigênio Dissolvido (OD.  No sistema foram plantadas duas culturas com destinação à alimentação animal as quais são a mucuna-preta (Mucuna pruriens (L. e o capim elefante (Pennisetum purpureum e uma para consumo humano, o maracujá (Passiflora sp. O tratamento das águas cinzas nas Unidades de Produção Agrícola Controladas promoveu uma diminuição da CE, pH e OD das águas oriundas da lavanderia, tornando uma alternativa viável e sustentável para o tratamento de água de lavanderias como também para produzir alimentos para fins animais e humanos.

  12. Microarray analysis identified Puccinia striiformis f. sp. tritici genes involved in infection and sporulation.

    Science.gov (United States)

    Puccinia striiformis f. sp. tritici (Pst) causes stripe rust, one of the most important diseases of wheat worldwide. To identify Pst genes involved in infection and sporulation, a custom oligonucleotide Genechip was made using sequences of 442 genes selected from Pst cDNA libraries. Microarray analy...

  13. Activation of the human beta interferon gene by the adenovirus type 12 E1B gene

    International Nuclear Information System (INIS)

    Shiroki, K.; Toth, M.

    1988-01-01

    The transcription of endogenous beta interferon mRNA was activated in human embryo kidney (HEK) cells infected with adenovirus 12 (Ad12) but was activated only inefficiently or not at all in HEK cells infected with Ad5 and rc-1 (Ad5 dl312 containing the Ad12 E1A region). The analysis with Ad12 mutants showed that Ad12 E1B products, especially the 19K protein, were important for the expression of the endogenous beta interferon gene and Ad12 E1A products were not involved in the expression. The expression of exogeneously transfected pIFN-CAT (a hybrid plasmid having the human beta interferon promoter fused with the CAT gene) was activated in HEK and chicken embryo fibroblast (CEF) cells infected with either Ad12 or Ad5. The analysis of cotransfection of CEF cells with pIFN-CAT and plasmids containing fragments of Ad12 or Ad5 DNA showed that Ad12 or Ad5 E1B (possibly the 19K protein) was and E1A was not involved in the expression of the exogenous pIFN-CAT

  14. AVALIAÇÃO SANITÁRIA E FÍSICO-QUÍMICA DAS ÁGUAS PARA IRRIGAÇÃO DE HORTALIÇAS NO AGRESTE E BREJO PARAIBANOS

    Directory of Open Access Journals (Sweden)

    Aldre Jorge Morais Barros

    1999-12-01

    Full Text Available RESUMO Foi avaliada a qualidade sanitária e físico-química das águas utilizadas na irrigação de hortaliças em cinco hortas localizadas em dois municípios paraibanos. As águas de irrigação apresentaram elevada contaminação fecal (CF: 104 UFC 100 mL-1 infectando os vegetais; esta contaminação teve como origem as descargas de esgotos domésticos nos córregos e açudes utilizados na irrigação, resultando no reúso indireto de esgotos na agricultura. As alfaces (Lactuca sativa L. irrigadas com tais águas apresentaram concentrações de coliformes fecais entre 1,0 x 103 e 1,6 x 106 e de E. coli entre 2,5 x 102 e 2,5 x 104 NMP g-1, indicando riscos para a saúde dos consumidores, porém as alfaces provenientes dos mercados públicos apresentaram carga fecal entre 7,5 x 103 e 3,8 x 104 NMP g-1, não havendo diferenças significativas com aquelas das hortas. A elevada carga fecal do adubo orgânico de origem animal (valor médio entre 1,3 x 105 e 2,6 x 106 NMP g-1 comumente utilizada nas hortas também contribuiu na contaminação das alfaces. Do ponto de vista físico-químico, as águas de irrigação continham apreciáveis concentrações de sais, porém não apresentaram altos risco de salinização ou de sodificação do solo.

  15. Involvement of the ornithine decarboxylase gene in acid stress response in probiotic Lactobacillus delbrueckii UFV H2b20.

    Science.gov (United States)

    Ferreira, A B; Oliveira, M N V de; Freitas, F S; Paiva, A D; Alfenas-Zerbini, P; Silva, D F da; Queiroz, M V de; Borges, A C; Moraes, C A de

    2015-01-01

    Amino acid decarboxylation is important for the maintenance of intracellular pH under acid stress. This study aims to carry out phylogenetic and expression analysis by real-time PCR of two genes that encode proteins involved in ornithine decarboxylation in Lactobacillus delbrueckii UFV H2b20 exposed to acid stress. Sequencing and phylogeny analysis of genes encoding ornithine decarboxylase and amino acid permease in L. delbrueckii UFV H2b20 showed their high sequence identity (99%) and grouping with those of L. delbrueckii subsp. bulgaricus ATCC 11842. Exposure of L. delbrueckii UFV H2b20 cells in MRS pH 3.5 for 30 and 60 min caused a significant increase in expression of the gene encoding ornithine decarboxylase (up to 8.1 times higher when compared to the control treatment). Increased expression of the ornithine decarboxylase gene demonstrates its involvement in acid stress response in L. delbrueckii UFV H2b20, evidencing that the protein encoded by that gene could be involved in intracellular pH regulation. The results obtained show ornithine decarboxylation as a possible mechanism of adaptation to an acidic environmental condition, a desirable and necessary characteristic for probiotic cultures and certainly important to the survival and persistence of the L. delbrueckii UFV H2b20 in the human gastrointestinal tract.

  16. Fitomassa e produção de algodoeiro cv. BRS Jady cultivado com águas salinas e doses de esterco bovino

    Directory of Open Access Journals (Sweden)

    Leandro de Pádua Souza

    2016-11-01

    Full Text Available A produção de algodoeiro de fibra naturalmente colorido, na região semiárida do nordeste brasileiro, onde as águas nem sempre são de boa qualidade, está na dependência do uso de técnicas que viabilizem o manejo do solo e da água com teor elevado de sais. Diante do exposto, objetivou-se avaliar fitomassa e produção do algodoeiro cv. BRS Jady submetido a níveis crescentes de salinidade da água de irrigação e doses de esterco bovino. O experimento foi conduzido em ambiente protegido em um Neossolo Regolítico Eutrófico de textura franco-arenosa no município de Campina Grande, Paraíba. Usou-se o delineamento experimental de blocos ao acaso, em esquema fatorial 4 x 4, com três repetições, sendo os tratamentos compostos de quatro níveis de condutividade elétrica da água (CEa (1,7; 3,4; 5,1 e 6,8 dS m-1 e quatro doses de esterco bovino (0; 2,5; 3,5 e 4,5% em base do volume do solo. Níveis crescentes de salinidade da água de irrigação com CEa superior a 1,7 dS m-1 reduziu a formação de fitomassa seca de folha, entretanto o aumento nas doses de esterco bovino promoveu acréscimos nesta variável. A adubação com esterco bovino promove incremento na produção de número de sementes totais e massa de cem sementes. Houve interação entre os fatores níveis de condutividade elétrica da água de irrigação e doses de esterco bovino para fitomassa seca e caule e raiz do algodoeiro cv. ‘BRS Jady’.Fitomassa and cotton production cv. BRS Jady cultivated with salt waters and esterco bovine dosesAbstract: The production of naturally colored fiber cotton in the semi-arid region of northeastern Brazil, where water is not always of good quality, is dependent on the use of techniques that make it possible to manage soil and water with high salt content. In view of the above, the objective was to evaluate phytomass and cotton production cv. BRS Jady subjected to increasing levels of salinity of irrigation water and doses of bovine

  17. Análise microbiana e do teor de flúor adicionado à água de abastecimento em um estado do Nordeste do Brasil

    Directory of Open Access Journals (Sweden)

    Guacyra Machado Lisboa

    2015-06-01

    Full Text Available Objetivo: Analisar os teores de íons de fluoreto e o padrão microbiológico encontrado nas águas de abastecimento público dos municípios alagoanos que fluoretam suas águas. Métodos: Estudo transversal descritivo realizado no período de 2012 a 2014, no qual se analisaram todos os laudos emitidos pelo Laboratório Central de Saúde Pública de Alagoas (LACEN-AL com os resultados da análise da qualidade da água para consumo humano dos onze municípios alagoanos que fluoretaram suas águas, totalizando 3.089 laudos. Avaliou-se o número total de amostras enviadas para análise da qualidade da água, a quantidade de amostras em que foi solicitada a análise de fluoreto, teores e variações dos íons de fluoreto e o padrão microbiológico das amostras fluoretadas. Realizou-se a análise descritiva dos dados, obtendo-se as frequências absolutas e relativas percentuais. Resultados: Foram encontradas 429 (83,9% amostras coletadas no intervalo 0,0-0,5 mgF/L, número considerado abaixo do recomendável, além de grande variação na concentração dos íons de fluoreto e 128 (26,3% amostras de água fluoretada fora dos padrões de potabilidade. Conclusão: Os dados mostraram uma grande variação e um alto percentual de amostras com baixas concentrações de fluoreto, assim como a necessidade de melhoria da qualidade da água ofertada, de modo a garantir para a população o acesso contínuo à água potável.

  18. Reconstruction of the gene regulatory network involved in the sonic hedgehog pathway with a potential role in early development of the mouse brain.

    Directory of Open Access Journals (Sweden)

    Jinhua Liu

    2014-10-01

    Full Text Available The Sonic hedgehog (Shh signaling pathway is crucial for pattern formation in early central nervous system development. By systematically analyzing high-throughput in situ hybridization data of E11.5 mouse brain, we found that Shh and its receptor Ptch1 define two adjacent mutually exclusive gene expression domains: Shh+Ptch1- and Shh-Ptch1+. These two domains are associated respectively with Foxa2 and Gata3, two transcription factors that play key roles in specifying them. Gata3 ChIP-seq experiments and RNA-seq assays on Gata3-knockdown cells revealed that Gata3 up-regulates the genes that are enriched in the Shh-Ptch1+ domain. Important Gata3 targets include Slit2 and Slit3, which are involved in the process of axon guidance, as well as Slc18a1, Th and Qdpr, which are associated with neurotransmitter synthesis and release. By contrast, Foxa2 both up-regulates the genes expressed in the Shh+Ptch1- domain and down-regulates the genes characteristic of the Shh-Ptch1+ domain. From these and other data, we were able to reconstruct a gene regulatory network governing both domains. Our work provides the first genome-wide characterization of the gene regulatory network involved in the Shh pathway that underlies pattern formation in the early mouse brain.

  19. Avaliação de um sistema de tratamento de águas cinzas em edificação de campus universitário

    OpenAIRE

    Mariana Cardoso Chrispim

    2014-01-01

    O reúso de água é um instrumento importante que contribui para a redução da pressão sobre os mananciais e para redução do consumo de água potável para fins que não requeiram água de padrão potável. Neste contexto, o presente estudo teve como objetivo principal analisar um sistema de coleta e tratamento de águas cinzas provenientes de pias de banheiro, chuveiros e máquina de lavar roupas tendo em vista o reúso não-potável em edificações. Os objetivos específicos foram: caracterizar qualitativa...

  20. Isolation and expression pattern of COR15b and KIN1 genes in ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-11-02

    Nov 2, 2009 ... COR15b and KIN1 (COR 6.5) genes encode polypeptides of 15 KDa and 6.5 KDa, respectively. They are involved in the dehydration tolerance mechanisms and play important role under cold stress. cDNA sequences of COR15b and KIN1 genes were firstly isolated from leaves of watermelon (Citrullus.

  1. E2F1-mediated transcriptional inhibition of the plasminogen activator inhibitor type 1 gene

    DEFF Research Database (Denmark)

    Koziczak, M; Müller, H; Helin, K

    2001-01-01

    but independent of binding to pocket-binding proteins, suggesting a novel mechanism for E2F-mediated negative gene regulation [Koziczak, M., Krek, W. & Nagamine, Y. (2000) Mol. Cell. Biol. 20, 2014-2022]. However, it remains to be seen whether endogenous E2F can exert a similar effect. We report here that down....... These results all indicate that endogenous E2F can directly repress the PAI-1 gene. DNase I hypersensitive-site analysis of the PAI-1 promoter suggested the involvement of conformation changes in chromatin structure of the PAI-1 promoter. 5' deletion analysis of the PAI-1 promoter showed that multiple sites...

  2. Identification of Candidate Genes Involved in the Salt Tolerance of Date Palm (Phoenix dactylifera L.) Based on a Yeast Functional Bioassay.

    Science.gov (United States)

    Patankar, Himanshu V; Al-Harrasi, Ibtisam; Al-Yahyai, Rashid; Yaish, Mahmoud W

    2018-06-01

    Although date palm is a relatively salt-tolerant plant, the molecular basis of this tolerance is complex and poorly understood. Therefore, this study aimed to identify the genes involved in salinity tolerance using a basic yeast functional bioassay. To achieve this, a date palm cDNA library was overexpressed in Saccharomyces cerevisiae cells. The expression levels of selected genes that make yeast cells tolerant to salt were subsequently validated in the leaf and root tissues of date palm seedlings using a quantitative PCR method. About 6000 yeast transformant cells were replica printed and screened on a synthetic minimal medium containing 1.0 M of NaCl. The screening results showed the presence of 62 salt-tolerant transformant colonies. Sequence analysis of the recombinant yeast plasmids revealed the presence of a group of genes with potential salt-tolerance functions, such as aquaporins (PIP), serine/threonine protein kinases (STKs), ethylene-responsive transcription factor 1 (ERF1), and peroxidases (PRX). The expression pattern of the selected genes endorsed the hypothesis that these genes may be involved in salinity tolerance, as they showed a significant (p < 0.05) overexpression trend in both the leaf and root tissues in response to salinity. The genes identified in this project are suitable candidates for the further functional characterization of date palms.

  3. Água de Coco comercializadas no Sertão do Ceará e Paraíba: Imprópria ao consumo

    Directory of Open Access Journals (Sweden)

    Suziane Alves Josino Lima

    2014-10-01

    Full Text Available A água de coco é considerada um isotônico natural, rica em nutrientes e altamente atrativa devido a seu valor nutricional e sabor, com a busca por alimentos saudáveis nos tempos atuais seu consumo tem aumentado consideravelmente principalmente na forma industrializada no Nordeste do Brasil onde se concentram os maiores plantios do coqueiro anão verde, tornando assim indispensável a avaliação da qualidade da água de coco comercializada nesta região. Neste sentido, este trabalho objetivou avaliar a qualidade microbiológica de amostras de água de coco, industrializada e comercializada por duas empresas no sertão paraibano e cearense. Como parâmetros da qualidade microbiológica foram realizadas as seguintes análises: Coliformes Termotolerantes,Salmonella spp/25g, Bolores e Leveduras e contagem de Microrganismos Aeróbios Psicrotróficos nas amostras. A água de coco anão verde comercializada pela indústria A, apresentou resultados de coliformes totais variando de 7 NMP/mL a 1,5 x 102 NMP/mL e < 3 NMP/mL a 7 NMP/mL para coliformes termotolerantes, estes valores oscilaram durante os trintas dias de armazenamento tempo este de validade do produto industrializado. Também foram detectados nas amostras analisadas uma alta contagem de bactérias, nas amostras da água de coco da indústria A armazenada sob-refrigeração, indicando assim possivelmente o uso de matéria prima contaminada, falhas no processamento distribuição e ou armazenamento, estas diretamente relacionadas ao binômio tempo/temperatura insatisfatórios. Os valores encontrados para Bolores e Leveduras variaram de 5,05x102 UFC/mL a 7,6x105 UFC/mL, valores estes considerados elevados, caracterizando a água de coco como imprópria para o consumo quando comparadas á legislação vigente. De acordo com os resultados sugere-se que esta contaminação tenha ocorrido durante a manipulação e ou processamento, uma vez que esta apresenta-se estéril dentro do seu próprio inv

  4. Reator anaeróbio compartimentado para o tratamento de águas residuárias de suinocultura

    Directory of Open Access Journals (Sweden)

    Roberto Alves de Oliveira

    2014-12-01

    Full Text Available A poluição da água e do solo causada pela suinocultura provoca impactos ambientais severos nas regiões produtoras. Uma alternativa eficiente para atenuar esse problema pode ser a utilização de reatores anaeróbios de alta taxa. Neste trabalho, avaliou-se o efeito das águas residuárias de suinocultura com concentrações de sólidos suspensos totais em torno de 6.000 mg.L−1 no desempenho do reator anaeróbio compartimentado, com três câmaras. O volume da primeira câmara foi de 210 L e da segunda e terceira de 160 L. O reator anaeróbio compartimentado foi submetido a tempos de detenção hidráulica de 56, 28 e 18 horas e a cargas orgânicas volumétricas de 5,0 a 10,1 g DQOtotal (L.d−1. As maiores remoções de demanda química de oxigênio total, de 42 a 68%, e das produções volumétricas de metano, de 0,261 a 0,454 L CH4 (L reator d−1, ocorreram na segunda câmara.

  5. The transcriptional repressor DREAM is involved in thyroid gene expression

    International Nuclear Information System (INIS)

    D'Andrea, Barbara; Di Palma, Tina; Mascia, Anna; Motti, Maria Letizia; Viglietto, Giuseppe; Nitsch, Lucio; Zannini, Mariastella

    2005-01-01

    Downstream regulatory element antagonistic modulator (DREAM) was originally identified in neuroendocrine cells as a calcium-binding protein that specifically binds to downstream regulatory elements (DRE) on DNA, and represses transcription of its target genes. To explore the possibility that DREAM may regulate the endocrine activity of the thyroid gland, we analyzed its mRNA expression in undifferentiated and differentiated thyroid cells. We demonstrated that DREAM is expressed in the normal thyroid tissue as well as in differentiated thyroid cells in culture while it is absent in FRT poorly differentiated cells. In the present work, we also show that DREAM specifically binds to DRE sites identified in the 5' untranslated region (UTR) of the thyroid-specific transcription factors Pax8 and TTF-2/FoxE1 in a calcium-dependent manner. By gel retardation assays we demonstrated that thapsigargin treatment increases the binding of DREAM to the DRE sequences present in Pax8 and TTF-2/Foxe1 5' UTRs, and this correlates with a significant reduction of the expression of these genes. Interestingly, in poorly differentiated thyroid cells overexpression of exogenous DREAM strongly inhibits Pax8 expression. Moreover, we provide evidence that a mutated form of DREAM unable to bind Ca 2+ interferes with thyroid cell proliferation. Therefore, we propose that in thyroid cells DREAM is a mediator of the calcium-signaling pathway and it is involved in the regulation of thyroid cell function

  6. Genes involved in protein metabolism of the probiotic lactic acid bacterium Lactobacillus delbrueckii UFV H2b20.

    Science.gov (United States)

    Do Carmo, A P; da Silva, D F; De Oliveira, M N V; Borges, A C; De Carvalho, A F; De Moraes, C A

    2011-09-01

    A basic requirement for the prediction of the potential use of lactic acid bacteria (LAB) in the dairy industry is the identification of specific genes involved in flavour-forming pathways. The probiotic Lactobacillus delbrueckii UFV H2b20 was submitted to a genetic characterisation and phylogenetic analysis of genes involved in protein catabolism. Eight genes belonging to this system were identified, which possess a closely phylogenetic relationship to NCFM strains representative, as it was demonstrated for oppC and oppBII, encoding oligopeptide transport system components. PepC, PepN, and PepX might be essential for growth of LAB, probiotic or not, since the correspondent genes are always present, including in L. delbrueckii UFV H2b20 genome. For pepX gene, a probable link between carbohydrate catabolism and PepX expression may exists, where it is regulated by PepR1/CcpA-like, a common feature between Lactobacillus strains and also in L. delbrueckii UFV H2b20. The well conserved evolutionary history of the ilvE gene is evidence that the pathways leading to branched-chain amino acid degradation, such as isoleucine and valine, are similar among L. delbrueckii subsp. bulgaricus strains and L. delbrueckii UFV H2b20. Thus, the involvement of succinate in flavour formation can be attributed to IlvE activity. The presence of aminopeptidase G in L. delbrueckii UFV H2b20 genome, which is absent in several strains, might improve the proteolytic activity and effectiveness. The nucleotide sequence encoding PepG revealed that it is a cysteine endopeptidase, belonging to Peptidase C1 superfamily; sequence analysis showed 99% identity with L. delbrueckii subsp. bulgaricus ATCC 11842 pepG, whereas protein sequence analysis revealed 100% similarity with PepG from the same organism. The present study proposes a schematic model to explain how the proteolytic system of the probiotic L. delbrueckii UFV H2b20 works, based on the components identified so far.

  7. Evaluation of NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 genes in familial colorectal cancer predisposition

    International Nuclear Information System (INIS)

    Broderick, Peter; Bagratuni, Tina; Vijayakrishnan, Jairam; Lubbe, Steven; Chandler, Ian; Houlston, Richard S

    2006-01-01

    The observation that germline mutations in the oxidative DNA damage repair gene MUTYH cause colorectal cancer (CRC) provides strong evidence that dysregulation of the base excision repair (BER) pathway influences disease susceptibility. It is conceivable that germline sequence variation in other BER pathway genes such as NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 also contribute to CRC susceptibility. To evaluate whether sequence variants of NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 genes might act as CRC susceptibility alleles, we screened the coding sequence and intron-exon boundaries of these genes in 94 familial CRC cases in which involvement of known genes had been excluded. Three novel missense variants were identified NEIL2 C367A, TDG3 A196G and UNG2 C262T in patients, which were not observed in 188 healthy control DNAs. We detected novel germline alterations in NEIL2, TDG and UNG patients with CRC. The results suggest a limited role for NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 in development of CRC

  8. Evaluation of NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 genes in familial colorectal cancer predisposition

    Energy Technology Data Exchange (ETDEWEB)

    Broderick, Peter; Bagratuni, Tina; Vijayakrishnan, Jairam; Lubbe, Steven; Chandler, Ian; Houlston, Richard S [Section of Cancer Genetics, Brookes Lawley Building, Institute of Cancer Research, 15 Cotswold Road, Sutton, Surrey, SM2 5NG (United Kingdom)

    2006-10-09

    The observation that germline mutations in the oxidative DNA damage repair gene MUTYH cause colorectal cancer (CRC) provides strong evidence that dysregulation of the base excision repair (BER) pathway influences disease susceptibility. It is conceivable that germline sequence variation in other BER pathway genes such as NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 also contribute to CRC susceptibility. To evaluate whether sequence variants of NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 genes might act as CRC susceptibility alleles, we screened the coding sequence and intron-exon boundaries of these genes in 94 familial CRC cases in which involvement of known genes had been excluded. Three novel missense variants were identified NEIL2 C367A, TDG3 A196G and UNG2 C262T in patients, which were not observed in 188 healthy control DNAs. We detected novel germline alterations in NEIL2, TDG and UNG patients with CRC. The results suggest a limited role for NTHL1, NEIL1, NEIL2, MPG, TDG, UNG and SMUG1 in development of CRC.

  9. AVALIAÇÃO DA QUALIDADE SANITÁRIA DA ÁGUA NA BACIA HIDROGRÁFICA DO RIO ALMADA – BA

    Directory of Open Access Journals (Sweden)

    Gustavo Barreto Franco

    2015-07-01

    Full Text Available A qualidade das águas superficiais pode refletir deficiências no saneamento básico de sua bacia de drenagem, bem como impactos resultantes da dinâmica de ocupação do solo e das atividades econômicas ali desenvolvidas. O objetivo desse trabalho foi avaliar a qualidade sanitária da água superficial na bacia hidrográfica do rio Almada, relacionando os resultados ao uso e ocupação do solo. Para tal, foram quantificados os parâmetros coliformes totais e Escherichia coli em amostras de água coletadas em doze pontos de amostragem da bacia, durante cinco campanhas realizadas em 2009 e 2010. Grande parte dos pontos apresentou resultados fora dos padrões estabelecidos pela Resolução CONAMA 357/05, referente a classe 2 para água doce, destacando-se os resultados obtidos nos trechos à jusante de centros urbanos. Os resultados demonstram a necessidade da melhoria da infraestrutura de saneamento e de um planejamento agroambiental nos municípios da bacia.

  10. Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration

    Directory of Open Access Journals (Sweden)

    Jessica A. Simpkins

    2016-06-01

    Full Text Available Cystine and cysteine are important molecules for pathways such as redox signaling and regulation, and thus identifying cellular deficits upon deletion of the Saccharomyces cerevisiae cystine transporter Ers1p allows for a further understanding of cystine homeostasis. Previous complementation studies using the human ortholog suggest yeast Ers1p is a cystine transporter. Human CTNS encodes the protein Cystinosin, a cystine transporter that is embedded in the lysosomal membrane and facilitates the export of cystine from the lysosome. When CTNS is mutated, cystine transport is disrupted, leading to cystine accumulation, the diagnostic hallmark of the lysosomal storage disorder cystinosis. Here, we provide biochemical evidence for Ers1p-dependent cystine transport. However, the accumulation of intracellular cystine is not observed when the ERS1 gene is deleted from ers1-Δ yeast, supporting the existence of modifier genes that provide a mechanism in ers1-Δ yeast that prevents or corrects cystine accumulation. Upon comparison of the transcriptomes of isogenic ERS1+ and ers1-Δ strains of S. cerevisiae by DNA microarray followed by targeted qPCR, sixteen genes were identified as being differentially expressed between the two genotypes. Genes that encode proteins functioning in sulfur regulation, cellular respiration, and general transport were enriched in our screen, demonstrating pleiotropic effects of ers1-Δ. These results give insight into yeast cystine regulation and the multiple, seemingly distal, pathways that involve proper cystine recycling.

  11. A novel contiguous deletion involving NDP, MAOB and EFHC2 gene ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Genetics; Volume 96; Issue 6. A novel contiguous deletion involving NDP, MAOBand EFHC2gene in a patient with familial Norrie disease: bilateral blindness and leucocoria without other deficits. BEI JIA LIPING HUANG YAOYU CHEN SIPING LIU CUIHUA CHEN KE XIONG LANLIN SONG YULAI ...

  12. A novel contiguous deletion involving NDP, MAOB and EFHC2 gene ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Genetics; Volume 96; Issue 6. A novel contiguous deletion involving NDP, MAOBitalic> and EFHC2italic> gene in a patient with familial Norrie disease: bilateral blindness and leucocoria without other deficits. BEI JIA LIPING HUANG YAOYU CHEN SIPING LIU CUIHUA CHEN KE XIONG LANLIN ...

  13. Identification and Heterologous Expression of Genes Involved in Anaerobic Dissimilatory Phosphite Oxidation by Desulfotignum phosphitoxidans▿

    Science.gov (United States)

    Simeonova, Diliana Dancheva; Wilson, Marlena Marie; Metcalf, William W.; Schink, Bernhard

    2010-01-01

    Desulfotignum phosphitoxidans is a strictly anaerobic, Gram-negative bacterium that utilizes phosphite as the sole electron source for homoacetogenic CO2 reduction or sulfate reduction. A genomic library of D. phosphitoxidans, constructed using the fosmid vector pJK050, was screened for clones harboring the genes involved in phosphite oxidation via PCR using primers developed based on the amino acid sequences of phosphite-induced proteins. Sequence analysis of two positive clones revealed a putative operon of seven genes predicted to be involved in phosphite oxidation. Four of these genes (ptxD-ptdFCG) were cloned and heterologously expressed in Desulfotignum balticum, a related strain that cannot use phosphite as either an electron donor or as a phosphorus source. The ptxD-ptdFCG gene cluster was sufficient to confer phosphite uptake and oxidation ability to the D. balticum host strain but did not allow use of phosphite as an electron donor for chemolithotrophic growth. Phosphite oxidation activity was measured in cell extracts of D. balticum transconjugants, suggesting that all genes required for phosphite oxidation were cloned. Genes of the phosphite gene cluster were assigned putative functions on the basis of sequence analysis and enzyme assays. PMID:20622064

  14. Identification and heterologous expression of genes involved in anaerobic dissimilatory phosphite oxidation by Desulfotignum phosphitoxidans.

    Science.gov (United States)

    Simeonova, Diliana Dancheva; Wilson, Marlena Marie; Metcalf, William W; Schink, Bernhard

    2010-10-01

    Desulfotignum phosphitoxidans is a strictly anaerobic, Gram-negative bacterium that utilizes phosphite as the sole electron source for homoacetogenic CO2 reduction or sulfate reduction. A genomic library of D. phosphitoxidans, constructed using the fosmid vector pJK050, was screened for clones harboring the genes involved in phosphite oxidation via PCR using primers developed based on the amino acid sequences of phosphite-induced proteins. Sequence analysis of two positive clones revealed a putative operon of seven genes predicted to be involved in phosphite oxidation. Four of these genes (ptxD-ptdFCG) were cloned and heterologously expressed in Desulfotignum balticum, a related strain that cannot use phosphite as either an electron donor or as a phosphorus source. The ptxD-ptdFCG gene cluster was sufficient to confer phosphite uptake and oxidation ability to the D. balticum host strain but did not allow use of phosphite as an electron donor for chemolithotrophic growth. Phosphite oxidation activity was measured in cell extracts of D. balticum transconjugants, suggesting that all genes required for phosphite oxidation were cloned. Genes of the phosphite gene cluster were assigned putative functions on the basis of sequence analysis and enzyme assays.

  15. Deficiência hídrica no consumo de água de dois cultivares de milho crescidos em solução nutritiva

    Directory of Open Access Journals (Sweden)

    Eduardo Caruso Machado

    1985-01-01

    Full Text Available Estudaram-se no presente trabalho os efeitos da deficiência hídrica no crescimento e consumo de água de plantas dos cultivares de milho IAC-Maya e IAC-Maya latente crescidos em casa de vegetação e em solução nutritiva em recipientes cilíndricos de PVC (diâmetro = 15cm, altura = 100cm, com capacidade para 15 litros. A solução nutritiva, composta de (mg/litro: Ca (284,N-NO3 (297, N-NH4 (37, K (140, P (6, Mg (41, Fe (7,5, B (0,5, Cu (0,08, Mn (1,0, Mo (0,16 e Zn (0,3, foi renovada a cada 30 dias. Nos estádios vegetativo e de florescimento, as plantas foram submetidas à deficiência hídrica através de a adição de polietileno glicol e b suspensão da reposição da água evapotranspirada pelas plantas. O delineamento estatístico foi o de blocos ao acaso com cinco repetições. A deficiência hídrica ocasionou, em ambos os cultivares, redução no consumo de água e na área foliar. Nos tratamentos onde a deficiência hídrica foi induzida pela suspensão da reposição da água evapotranspirada, observou-se aumento das raízes de ambos os cultivares. Os resultados sugerem que a técnica de cultivo por hidroponia associada ao uso de agentes osmóticos e/ou suspensão da reposição da água evapotranspirada, pode ser utilizada em estudos de deficiência hídrica em plantas.

  16. Tendências temporais e espaciais da qualidade das águas superficiais da sub-bacia do Rio das Velhas, estado de Minas Gerais

    Directory of Open Access Journals (Sweden)

    Ana Laura Cerqueira Trindade

    Full Text Available RESUMO Este artigo apresenta uma análise da tendência temporal e espacial da qualidade das águas superficiais da sub-bacia do Rio das Velhas, inserida na bacia do Rio São Francisco, em Minas Gerais, Brasil. Foram analisados 16.625 dados coletados no período de 2002 a 2011 pelo programa de monitoramento de qualidade das águas superficiais efetuado pelo Instituto Mineiro de Gestão das Águas (Igam. Testes estatísticos, multivariados e não paramétricos foram utilizados para avaliar 11 variáveis físicas, químicas e microbiológicas de 29 estações de monitoramento. Os resultados das análises de tendência Mann-Kendall/Sazonal de Mann-Kendall sugeriram que a maioria dos cursos d'água da região apresentam valores estáveis das variáveis ao longo do período estudado, com maiores alterações associadas a coliformes termotolerantes, demanda bioquímica de oxigênio (DBO, nitrato e índice de qualidade das águas (IQA, principalmente nas proximidades dos grandes centros urbanos. A análise de Cluster definiu três grandes grupos de estações de monitoramento, agrupadas segundo a qualidade de suas águas, correspondentes aos níveis de alta poluição, poluição moderada e baixa poluição. No entanto, ao longo de toda a sub-bacia, foi observada a degradação da qualidade da água durante o período estudado, principalmente relacionada ao lançamento de esgotos domésticos. Uma importante constatação foi a alteração da qualidade da água no baixo Rio das Velhas, apontada pela tendência de redução do IQA em estações localizadas nessa região, o que merece atenção dos órgãos governamentais para ações de manejo.

  17. Tendências temporais e espaciais da qualidade das águas superficiais da sub-bacia do Rio das Velhas, estado de Minas Gerais

    Directory of Open Access Journals (Sweden)

    Ana Laura Cerqueira Trindade

    2016-10-01

    Full Text Available RESUMO Este artigo apresenta uma análise da tendência temporal e espacial da qualidade das águas superficiais da sub-bacia do Rio das Velhas, inserida na bacia do Rio São Francisco, em Minas Gerais, Brasil. Foram analisados 16.625 dados coletados no período de 2002 a 2011 pelo programa de monitoramento de qualidade das águas superficiais efetuado pelo Instituto Mineiro de Gestão das Águas (Igam. Testes estatísticos, multivariados e não paramétricos foram utilizados para avaliar 11 variáveis físicas, químicas e microbiológicas de 29 estações de monitoramento. Os resultados das análises de tendência Mann-Kendall/Sazonal de Mann-Kendall sugeriram que a maioria dos cursos d'água da região apresentam valores estáveis das variáveis ao longo do período estudado, com maiores alterações associadas a coliformes termotolerantes, demanda bioquímica de oxigênio (DBO, nitrato e índice de qualidade das águas (IQA, principalmente nas proximidades dos grandes centros urbanos. A análise de Cluster definiu três grandes grupos de estações de monitoramento, agrupadas segundo a qualidade de suas águas, correspondentes aos níveis de alta poluição, poluição moderada e baixa poluição. No entanto, ao longo de toda a sub-bacia, foi observada a degradação da qualidade da água durante o período estudado, principalmente relacionada ao lançamento de esgotos domésticos. Uma importante constatação foi a alteração da qualidade da água no baixo Rio das Velhas, apontada pela tendência de redução do IQA em estações localizadas nessa região, o que merece atenção dos órgãos governamentais para ações de manejo.

  18. CRTC1 Nuclear Translocation Following Learning Modulates Memory Strength via Exchange of Chromatin Remodeling Complexes on the Fgf1 Gene.

    Science.gov (United States)

    Uchida, Shusaku; Teubner, Brett J W; Hevi, Charles; Hara, Kumiko; Kobayashi, Ayumi; Dave, Rutu M; Shintaku, Tatsushi; Jaikhan, Pattaporn; Yamagata, Hirotaka; Suzuki, Takayoshi; Watanabe, Yoshifumi; Zakharenko, Stanislav S; Shumyatsky, Gleb P

    2017-01-10

    Memory is formed by synapse-to-nucleus communication that leads to regulation of gene transcription, but the identity and organizational logic of signaling pathways involved in this communication remain unclear. Here we find that the transcription cofactor CRTC1 is a critical determinant of sustained gene transcription and memory strength in the hippocampus. Following associative learning, synaptically localized CRTC1 is translocated to the nucleus and regulates Fgf1b transcription in an activity-dependent manner. After both weak and strong training, the HDAC3-N-CoR corepressor complex leaves the Fgf1b promoter and a complex involving the translocated CRTC1, phosphorylated CREB, and histone acetyltransferase CBP induces transient transcription. Strong training later substitutes KAT5 for CBP, a process that is dependent on CRTC1, but not on CREB phosphorylation. This in turn leads to long-lasting Fgf1b transcription and memory enhancement. Thus, memory strength relies on activity-dependent changes in chromatin and temporal regulation of gene transcription on specific CREB/CRTC1 gene targets. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

  19. The novel lipopeptide poaeamide of the endophyte Pseudomonas poae RE*1-1-14 is involved in pathogen suppression and root colonization

    NARCIS (Netherlands)

    Zachow, C.; Jahanshah, G.; Bruijn, de I.; Song, C.; Ianni, F.; Pataj, Z.; Gerhardt, H.; Pianet, I.; Lämmerhofer, M.; Berg, G.; Gross, H.; Raaijmakers, J.M.

    2015-01-01

    Endophytic Pseudomonas poae strain RE*1-1-14 was originally isolated from internal root tissue of sugar beet plants and shown to suppress growth of the fungal pathogen Rhizoctonia solani both in vitro and in the field. To identify genes involved in its biocontrol activity, RE*1-1-14 random

  20. The novel lipopeptide Poaeamide of the endophyte Pseudomonas poae RE*1-1-14 is involved in pathogen suppression and root colonization

    NARCIS (Netherlands)

    Zachow, Christin; Jahanshah, Ghazaleh; de Bruijn, Irene; Song, Chunxu; Ianni, Federica; Pataj, Zoltán; Gerhardt, Heike; Pianet, Isabelle; Lämmerhofer, Michael; Berg, Gabriele; Gross, Harald; Raaijmakers, Jos M.

    2015-01-01

    Endophytic Pseudomonas poae strain RE*1-1-14 was originally isolated from internal root tissue of sugar beet plants and shown to suppress growth of the fungal pathogen Rhizoctonia solani both in vitro and in the field. To identify genes involved in its biocontrol activity, RE*1-1-14 random

  1. Regulation of UGT1A1 and HNF1 transcription factor gene expression by DNA methylation in colon cancer cells

    Directory of Open Access Journals (Sweden)

    Harvey Mario

    2010-01-01

    Full Text Available Abstract Background UDP-glucuronosyltransferase 1A1 (UGT1A1 is a pivotal enzyme involved in metabolism of SN-38, the active metabolite of irinotecan commonly used to treat metastatic colorectal cancer. We previously demonstrated aberrant methylation of specific CpG dinucleotides in UGT1A1-negative cells, and revealed that methylation state of the UGT1A1 5'-flanking sequence is negatively correlated with gene transcription. Interestingly, one of these CpG dinucleotides (CpG -4 is found close to a HNF1 response element (HRE, known to be involved in activation of UGT1A1 gene expression, and within an upstream stimulating factor (USF binding site. Results Gel retardation assays revealed that methylation of CpG-4 directly affect the interaction of USF1/2 with its cognate sequence without altering the binding for HNF1-alpha. Luciferase assays sustained a role for USF1/2 and HNF1-alpha in UGT1A1 regulation in colon cancer cells. Based on the differential expression profiles of HNF1A gene in colon cell lines, we also assessed whether methylation affects its expression. In agreement with the presence of CpG islands in the HNF1A promoter, treatments of UGT1A1-negative HCT116 colon cancer cells with a DNA methyltransferase inhibitor restore HNF1A gene expression, as observed for UGT1A1. Conclusions This study reveals that basal UGT1A1 expression in colon cells is positively regulated by HNF1-alpha and USF, and negatively regulated by DNA methylation. Besides, DNA methylation of HNF1A could also play an important role in regulating additional cellular drug metabolism and transporter pathways. This process may contribute to determine local inactivation of drugs such as the anticancer agent SN-38 by glucuronidation and define tumoral response.

  2. Efeito da adubação orgânica sobre a matéria seca do girassol irrigado com água residuária

    Directory of Open Access Journals (Sweden)

    Tainara Tâmara Santiago Silva

    2016-08-01

    Full Text Available Objetivou-se com este trabalho avaliar os efeitos da irrigação com água residuária doméstica tratada e da adubação com composto de resíduo sólido urbano na matéria seca de plantas de girassol. O experimento foi conduzido em casa de vegetação na Universidade Federal de Campina Grande – PB; a cultura utilizada foi o Girassol (Helianthus annuus L., variável EMBRAPA 122/V2000, cultivadas em vasos preenchidos com solo classificado como Neossolo Regolítico eutrófico. O delineamento experimental foi em blocos inteiramente casualizados em esquema fatorial 6 x 2, com 3 repetições, que foram 6 doses de nitrogênio disponível em composto de resíduo sólido (0, 60, 100, 140, 180, 220 kg. N. ha-1 e 2 tipos de águagua de abastecimento e água residuária doméstica tratada. A água residuária foi tratada por meio de reator UASB (Reator anaeróbico de fluxo ascendente e a água de abastecimento foi proveniente do sistema de abastecimento público municipal. Verificou-se que a qualidade de ág