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Sample records for gro gro radi

  1. Myxococcus xanthus DK1622 Coordinates Expressions of the Duplicate groEL and Single groES Genes for Synergistic Functions of GroELs and GroES

    Directory of Open Access Journals (Sweden)

    Yue-zhong Li

    2017-04-01

    Full Text Available Chaperonin GroEL (Cpn60 requires cofactor GroES (Cpn10 for protein refolding in bacteria that possess single groEL and groES genes in a bicistronic groESL operon. Among 4,861 completely-sequenced prokaryotic genomes, 884 possess duplicate groEL genes and 770 possess groEL genes with no neighboring groES. It is unclear whether stand-alone groEL requires groES in order to function and, if required, how duplicate groEL genes and unequal groES genes balance their expressions. In Myxococcus xanthus DK1622, we determined that, while duplicate groELs were alternatively deletable, the single groES that clusters with groEL1 was essential for cell survival. Either GroEL1 or GroEL2 required interactions with GroES for in vitro and in vivo functions. Deletion of groEL1 or groEL2 resulted in decreased expressions of both groEL and groES; and ectopic complementation of groEL recovered not only the groEL but also groES expressions. The addition of an extra groES gene upstream groEL2 to form a bicistronic operon had almost no influence on groES expression and the cell survival rate, whereas over-expression of groES using a self-replicating plasmid simultaneously increased the groEL expressions. The results indicated that M. xanthus DK1622 cells coordinate expressions of the duplicate groEL and single groES genes for synergistic functions of GroELs and GroES. We proposed a potential regulation mechanism for the expression coordination.

  2. Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES

    Science.gov (United States)

    Chi, Haixia; Wang, Xiaoqiang; Li, Jiqiang; Ren, Hao; Huang, Fang

    2015-11-01

    The in vitro folding of newly translated human CC chemokine receptor type 5 (CCR5), which belongs to the physiologically important family of G protein-coupled receptors (GPCRs), has been studied in a cell-free system supplemented with the surfactant Brij-35. The freshly synthesized CCR5 can spontaneously fold into its biologically active state but only slowly and inefficiently. However, on addition of the GroEL-GroES molecular chaperone system, the folding of the nascent CCR5 was significantly enhanced, as was the structural stability and functional expression of the soluble form of CCR5. The chaperonin GroEL was partially effective on its own, but for maximum efficiency both the GroEL and its GroES lid were necessary. These results are direct evidence for chaperone-assisted membrane protein folding and therefore demonstrate that GroEL-GroES may be implicated in the folding of membrane proteins.

  3. GRoW Buffalo Final Report

    Energy Technology Data Exchange (ETDEWEB)

    Bohm, Martha [Univ. at Buffalo, NY (United States)

    2016-04-17

    This document provides final reporting on the GRoW Home, University at Buffalo's entry to the 2015 Solar Decathlon competition in Irvine, CA. The report summarizes fundraising efforts, documents media outreach, lists online presence, analyzes the organizer's communication, describes post-competition life of the house and future employment plans for student team members. Last, it suggests improvements for future decathlons.

  4. Progress in molecular chaperon GroEL%GroEL分子伴侣研究进展

    Institute of Scientific and Technical Information of China (English)

    张经余; 赵志虎; 蔡民华

    2001-01-01

    大肠杆菌的GroEL是同型寡聚复合体,由14个相对分子质量58×103亚基组成背靠背的双环结构。它在新生蛋白质的正确折叠和组装以及在热或化学逆境下变性蛋白质的恢复过程中起重要作用。同时,在大肠杆菌的跨膜转运及插入细胞质膜方面都起重要作用。这些活动依赖于GroEL与底物蛋白的疏水片断的相互作用。综述了Hsp60分子伴侣系统中研究得比较清楚的GroEL的晶体结构、功能及作用机理等方面的研究进展。%Molecular chaperon GroEL of E.coli is a homo-oligomeric complex composed of 14 58kDa subunits arranged in two rings stacked back to back, which is required for correct folding and assambly of newly synthesized proteins and for recovery of the cell after exposure to either thermal or chemical stress .Apart from these functions, GroEL chaperon is able to play a role in protein translocation across or insertion into the cytoplasmic membrane of E.coli. These actions depend on the interaction between GroE chaperionin system and the hydrophobic side chains .

  5. Structural features of the GroEL-GroES nano-cage required for rapid folding of encapsulated protein

    DEFF Research Database (Denmark)

    Tang, Sheila Tuyet; Chang, HC; Roeben, A

    2006-01-01

    GroEL and GroES form a chaperonin nano-cage for proteins up to similar to 60 kDa to fold in isolation. Here we explored the structural features of the chaperonin cage critical for rapid folding of encapsulated substrates. Modulating the volume of the GroEL central cavity affected folding speed....... Additionally, interactions with the C-terminal, mildly hydrophobic Gly-Gly-Met repeat sequences of GroEL protruding into the cavity, and repulsion effects from the negatively charged cavity wall were required for rapid folding of some proteins. We suggest that by combining these features, the chaperonin cage...

  6. Sequence analysis of the Legionella micdadei groELS operon

    DEFF Research Database (Denmark)

    Hindersson, P; Høiby, N; Bangsborg, Jette Marie

    1991-01-01

    shock expression signals were identified upstream of the L. micdadei groEL gene. Further upstream, a poly-T region, also a feature of the sigma 32-regulated Escherichia coli groELS heat shock operon, was found. Despite the high degree of homology of the expression signals in E. coli and L. micdadei...

  7. Public health lives: Gro Harlem Brundtland.

    Science.gov (United States)

    Yach, D; von Schirnding, Y

    2014-02-01

    Health has been a deeply personal, professional and political dimension of Gro Harlem Brundtland's life. Her decision to study breast feeding while an MPH student at Harvard in 1964, or her desire to tackle tobacco being influenced by her father sending her as a 10-year old girl to buy his cigarettes at the local store, or her deeply personal family experience of mental ill health all led her to take actions on the global stage to address these and other issues that evidence showed would have global impact. Her impact on global health started with a commitment to make a difference in the lives of people, particularly those in greatest need. Copyright © 2013 The Royal Society for Public Health. Published by Elsevier Ltd. All rights reserved.

  8. GroEL of the nitrogen-fixing cyanobacterium Anabaena sp. strain L-31 exhibits GroES and ATP-independent refolding activity.

    Science.gov (United States)

    Potnis, Akhilesh A; Rajaram, Hema; Apte, Shree K

    2016-03-01

    The nitrogen-fixing cyanobacterium, Anabaena L-31 has two Hsp60 proteins, 59 kDa GroEL coded by the second gene of groESL operon and 61 kDa Cpn60 coded by cpn60 gene. Anabaena GroEL formed stable higher oligomer (>12-mer) in the presence of K(+) and prevented thermal aggregation of malate dehydrogenase (MDH). Using three protein substrates (MDH, All1541 and green fluorescent protein), it was found that the refolding activity of Anabaena GroEL was lower than that of Escherichia coli GroEL, but independent of both GroES and ATP. This correlated with in vivo data. GroEL exhibited ATPase activity which was enhanced in the presence of GroES and absence of a denatured protein, contrary to that observed for bacterial GroEL. However, a significant role for ATP could not be ascertained during in vitro folding assays. The monomeric Cpn60 exhibited much lower refolding activity than GroEL, unaffected by GroES and ATP. In vitro studies revealed inhibition of the refolding activity of Anabaena GroEL by Cpn60, which could be due to their different oligomeric status. The role of GroES and ATP may have been added during the course of evolution from the ancient cyanobacteria to modern day bacteria enhancing the refolding ability and ensuring wider scope of substrates for GroEL.

  9. Dataset concerning GroEL chaperonin interaction with proteins

    Directory of Open Access Journals (Sweden)

    V.V. Marchenkov

    2016-03-01

    Full Text Available GroEL chaperonin is well-known to interact with a wide variety of polypeptide chains. Here we show the data related to our previous work (http://dx.doi.org/10.1016/j.pep.2015.11.020 [1], and concerning the interaction of GroEL with native (lysozyme, α-lactalbumin and denatured (lysozyme, α-lactalbumin and pepsin proteins in solution. The use of affinity chromatography on the base of denatured pepsin for GroEL purification from fluorescent impurities is represented as well.

  10. Strategisches Informationsmanagement in Großprojekten der Industrie

    OpenAIRE

    Gemmel, Gunter

    2013-01-01

    Unternehmen gründen Projekte, um einmalige, zeitlich begrenzte Vorhaben effizient zu realisieren. Gerade in der Industrie zeigt sich diese Vorgehensweise häufig in Form von Großprojekten. Das IT-Management zur Unterstützung dieser Industrieprojekte steht dabei vor besonderen, charakteristischen Herausforderungen. Diese Arbeit beantwortet die Forschungsfrage, wie die bestehenden Konzepte des strategi- schen Informationsmanagements im speziellen Kontext von Großprojekten der Industrie angewe...

  11. GroEL mediates protein folding with a two successive timer mechanism.

    Science.gov (United States)

    Ueno, Taro; Taguchi, Hideki; Tadakuma, Hisashi; Yoshida, Masasuke; Funatsu, Takashi

    2004-05-21

    GroEL encapsulates nonnative substrate proteins in a central cavity capped by GroES, providing a safe folding cage. Conventional models assume that a single timer lasting approximately 8 s governs the ATP hydrolysis-driven GroEL chaperonin cycle. We examine single molecule imaging of GFP folding within the cavity, binding release dynamics of GroEL-GroES, ensemble measurements of GroEL/substrate FRET, and the initial kinetics of GroEL ATPase activity. We conclude that the cycle consists of two successive timers of approximately 3 s and approximately 5 s duration. During the first timer, GroEL is bound to ATP, substrate protein, and GroES. When the first timer ends, the substrate protein is released into the central cavity and folding begins. ATP hydrolysis and phosphate release immediately follow this transition. ADP, GroES, and substrate depart GroEL after the second timer is complete. This mechanism explains how GroES binding to a GroEL-substrate complex encapsulates the substrate rather than allowing it to escape into solution.

  12. Manuelle Montage von Großgeräten

    Science.gov (United States)

    Lotter, Bruno

    Die manuelle Montage von Großgeräten — wie. z. B. Maschinen, Traktoren, Haushaltsgeräte, PKW-Motoren, LKW-Achsen, Schaltschränke usw. — wird in ihrer Montagestruktur vorrangig durch das Produktgewicht, die Produktabmessung und die Produktionsrate bestimmt.

  13. Probing the dynamic process of encapsulation in Escherichia coli GroEL.

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    Toshifumi Mizuta

    Full Text Available Kinetic analyses of GroE-assisted folding provide a dynamic sequence of molecular events that underlie chaperonin function. We used stopped-flow analysis of various fluorescent GroEL mutants to obtain details regarding the sequence of events that transpire immediately after ATP binding to GroEL and GroEL with prebound unfolded proteins. Characterization of GroEL CP86, a circularly permuted GroEL with the polypeptide ends relocated to the vicinity of the ATP binding site, showed that GroES binding and protection of unfolded protein from solution is achieved surprisingly early in the functional cycle, and in spite of greatly reduced apical domain movement. Analysis of fluorescent GroEL SR-1 and GroEL D398A variants suggested that among other factors, the presence of two GroEL rings and a specific conformational rearrangement of Helix M in GroEL contribute significantly to the rapid release of unfolded protein from the GroEL apical domain.

  14. Differential T-cell recognition of native and recombinant Mycobacterium tuberculosis GroES

    DEFF Research Database (Denmark)

    Rosenkrands, I; Weldingh, K; Ravn, P

    1999-01-01

    Mycobacterium tuberculosis GroES was purified from culture filtrate, and its identity was confirmed by immunoblot analysis and N-terminal sequencing. Comparing the immunological recognition of native and recombinant GroES, we found that whereas native GroES elicited a strong proliferative response...

  15. Chaperonin GroEL/GroES Over-Expression Promotes Aminoglycoside Resistance and Reduces Drug Susceptibilities in Escherichia coli Following Exposure to Sublethal Aminoglycoside Doses

    DEFF Research Database (Denmark)

    Goltermann, Lise; Sarusie, Menachem V; Bentin, Thomas

    2016-01-01

    Antibiotic resistance is an increasing challenge to modern healthcare. Aminoglycoside antibiotics cause translation corruption and protein misfolding and aggregation in Escherichia coli. We previously showed that chaperonin GroEL/GroES depletion and over-expression sensitize and promote short-ter...... mechanism for emergence of antibiotic resistance.......Antibiotic resistance is an increasing challenge to modern healthcare. Aminoglycoside antibiotics cause translation corruption and protein misfolding and aggregation in Escherichia coli. We previously showed that chaperonin GroEL/GroES depletion and over-expression sensitize and promote short......-term tolerance, respectively, to this drug class. Here, we show that chaperonin GroEL/GroES over-expression accelerates acquisition of streptomycin resistance and reduces susceptibility to several other antibiotics following sub-lethal streptomycin antibiotic exposure. Chaperonin buffering could provide a novel...

  16. Chaperonin GroEL/GroES over-expression promotes multi-drug resistance in E. coli following exposure to aminoglycoside antibiotics

    Directory of Open Access Journals (Sweden)

    Lise eGoltermann

    2016-01-01

    Full Text Available Antibiotic resistance is an increasing challenge to modern healthcare. Aminoglycoside antiobiotics cause translation corruption and protein misfolding and aggregation in Escherichia coli. We previously showed that chaperonin GroEL/GroES depletion and overexpression sensitize and promote short-term tolerance, respectively, to this drug class. Here we show that chaperonin GroEL/GroES over-expression accelerates acquisition of aminoglycoside resistance and multi-drug resistance following sub-lethal aminoglycoside antibiotic exposure. Chaperonin buffering could provide a novel mechanism for antibiotic resistance and multi-drug resistance development.

  17. Cloning and characterization of groESL operon in Acetobacter aceti.

    Science.gov (United States)

    Okamoto-Kainuma, Akiko; Yan, Wang; Kadono, Sachiko; Tayama, Kenji; Koizumi, Yukimichi; Yanagida, Fujiharu

    2002-01-01

    The groESL operon of Acetobacter aceti was cloned and sequenced. We observed that GroES and GroEL of A. aceti had high amino acid sequence homologies to GroES and GroEL of Escherichia coli and Bacillus subtilis. The upstream region of the groESL operon contained the heat-shock promoter, which was previously reported in alpha-purple proteobacteria, and the highly conserved inverted repeat sequence. Phylogenetic analysis revealed that the A. aceti GroES and GroEL are very closely related to those of other alpha-purple proteobacteria. Transcription of this operon in A. aceti was induced by heat shock as well as by exposure to ethanol and acetic acid, which are present during fermentation of acetic acid. A. aceti that overexpressed the groESL was more resistant than the control strain to Stressors such as heat, ethanol, or acetic acid, indicating that GroES and GroEL are closely associated with the characteristic nature of Acetobacter and play an important role in acetic acid fermentation.

  18. Mimicking the action of GroEL in molecular dynamics simulations : Application to the refinement of protein structures

    NARCIS (Netherlands)

    Fan, H; Mark, AE

    2006-01-01

    Bacterial chaperonin, GroEL, together with its co-chaperonin, GroES, facilitates the folding of a variety of polypeptides. Experiments suggest that GroEL stimulates protein folding by multiple cycles of binding and release. Misfolded proteins first bind to an exposed hydrophobic surface on GroEL. Gr

  19. The runaway black hole GRO J1655-40

    CERN Document Server

    Mirabel, I F; Rodrigues, I; Combi, J A; Rodríguez, L F; Guglielmetti, F

    2002-01-01

    We have used the Hubble Space Telescope to measure the motion in the sky and compute the galactocentric orbit of the black hole X-ray binary GRO J1655-40. The system moves with a runaway space velocity of $112\\pm 18$ km s$^{-1}$ in a highly eccentric ($e = 0.34\\pm 0.05$) orbit. The black hole was formed in the disk at a distance greater than 3 kpc from the Galactic centre and must have been shot to such an eccentric orbit by the explosion of the progenitor star. The runaway linear momentum and kinetic energy of this black hole binary are comparable to those of solitary neutron stars and millisecond pulsars. GRO J1655-40 is the first black hole for which there is evidence for a runaway motion imparted by a natal kick in a supernova explosion.

  20. Asp-52 in combination with Asp-398 plays a critical role in ATP hydrolysis of chaperonin GroEL.

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    Koike-Takeshita, Ayumi; Mitsuoka, Kaoru; Taguchi, Hideki

    2014-10-24

    The Escherichia coli chaperonin GroEL is a double-ring chaperone that assists protein folding with the aid of GroES and ATP. Asp-398 in GroEL is known as one of the critical residues on ATP hydrolysis because GroEL(D398A) mutant is deficient in ATP hydrolysis (Asp-52 in the corresponding E. coli GroEL, in addition to Asp-398 is also important for ATP hydrolysis. We investigated the role of Asp-52 in GroEL and found that ATPase activity of GroEL(D52A) and GroEL(D52A/D398A) mutants was ∼ 20% and Asp-52 in E. coli GroEL is also involved in the ATP hydrolysis. GroEL(D52A/D398A) formed a symmetric football-shaped GroEL-GroES complex in the presence of ATP, again confirming the importance of the symmetric complex during the GroEL ATPase cycle. Notably, the symmetric complex of GroEL(D52A/D398A) was extremely stable, with a half-time of ∼ 150 h (∼ 6 days), providing a good model to characterize the football-shaped complex. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

  1. [Role of GroEL/GroES chaperonin system and Lon protease in regulation of expression Vibrio fischeri lux genes in Escherichia coli cells].

    Science.gov (United States)

    Manukhov, I V; Kotova, V Iu; Zavil'genskiĭ, G B

    2006-01-01

    It was shown that the chaperonin GroEL/GroES and protease Lon influence the expression of the Vibrio fischeri lux regulon in Escherichia coli cells: E. coli groE mutants bearing hybrid plasmid with the lux regulon were weakly luminescent; cells of the E. coli lon- comprising the entire lux regulon display very intense bioluminescence, with no lag period in the induction curve characteristic of lon+ strains. The luxR gene was cloned from the Vibrio fischeri genome in the pGEX-KG vector. It was shown that the active fusion protein GST-LuxR by affinity chromatography on glutathione-sucrose colony is purified only with proteins GroEL and Lon. The present results showed that the LuxR, transcriptional activator of the V. fischeri lux operon, really complexes with GroEL chaperonin and Lon protease. We suppose, that the GroEL/GroES chaperonin systems is required for the folding of LuxR into an active protein, and the LuxR is the target for the ATP-dependent serine Lon protease of E. coli.

  2. Tannerella forsythia GroEL induces inflammatory bone resorption and synergizes with interleukin-17.

    Science.gov (United States)

    Jung, Y-J; Choi, Y-J; An, S-J; Lee, H-R; Jun, H-K; Choi, B-K

    2016-08-03

    Tannerella forsythia is a major periodontal pathogen, and T. forsythia GroEL is a molecular chaperone homologous to human heat-shock protein 60. Interleukin-17 (IL-17) has been implicated in the pathogenesis of periodontitis and several systemic diseases. This study investigated the potential of T. forsythia GroEL to induce inflammatory bone resorption and examined the cooperative effect of IL-17 and T. forsythia GroEL on inflammatory responses. Human gingival fibroblasts (HGFs) and periodontal ligament (PDL) fibroblasts were stimulated with T. forsythia GroEL and/or IL-17. Gene expression of IL-6, IL-8, and cyclooxygenase-2 (COX-2) and concentrations of IL-6, IL-8, and prostaglandin E2 (PGE2 ) were measured by real-time reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assays, respectively. After stimulation of MG63 cells with T. forsythia GroEL and/or IL-17, gene expression of osteoprotegerin (OPG) was examined. After subcutaneous injection of T. forsythia GroEL and/or IL-17 above the calvaria of BALB/c mice, calvarial bone resorption was assessed by micro-computed tomography and histological examination. Tannerella forsythia GroEL induced IL-6 and IL-8 production in HGFs and PDL cells, and IL-17 further promoted IL-6 and IL-8 production. Both T. forsythia GroEL and IL-17 synergistically increased PGE2 production and inhibited OPG gene expression. Calvarial bone resorption was induced by T. forsythia GroEL injection, and simultaneous injection of T. forsythia GroEL and IL-17 further increased bone resorption. These results suggest that T. forsythia GroEL is a novel virulence factor that can contribute to inflammatory bone resorption caused by T. forsythia and synergizes with IL-17 to exacerbate inflammation and bone resorption.

  3. Induction of heat shock proteins DnaK, GroEL, and GroES by salt stress in Lactococcus lactis

    DEFF Research Database (Denmark)

    Kilstrup, Mogens; Jacobsen, Susanne; Hammer, Karin

    1997-01-01

    laboratory strain MG1363, which was originally derived from a dairy strain, After two-dimensional separation of proteins, the DnaK, GroEL, and GroES heat shock proteins, the HrcA (Orf1) heat shack repressor, and the glycolytic enzymes pyruvate kinase, glyceral-dehyde-3-phosphate dehydrogenase......, and phosphoglycerate kinase were identified by a combination of Western blotting and direct N-terminal amino acid sequencing of proteins from the gels, Of 400 to 500 visible proteins, 17 were induced more than twofold during heat stress, Two classes of heat stress proteins were identified from their temporal induction...... proteins exhibited a gradual increase in the rate of synthesis after the onset of stress, Unlike other organisms, all salt stress-induced proteins in L. lactis were also subjected to heat stress induction. DnaK, GroEL, and GroES showed similar temporal patterns of induction during salt stress, resembling...

  4. Localization of GroEL determined by in vivo incorporation of a fluorescent amino acid

    DEFF Research Database (Denmark)

    Charbon, Godefroid; Wang, Jiangyun; Brustad, Eric

    2011-01-01

    Escherichia coli cells, indicated colocalization with the cell division protein FtsZ at the cleavage furrow, while a second E. coli study of fixed cells indicated more even distribution throughout the cytoplasm. Here, for the first time, we have examined the spatial distribution of GroEL in living cells using......The molecular chaperone GroEL is required for bacterial growth under all conditions, mediating folding assistance, via its central cavity, to a diverse set of cytosolic proteins; yet the subcellular localization of GroEL remains unresolved. An earlier study, using antibody probing of fixed...... incorporation of a fluorescent unnatural amino acid into the chaperone. Fluorescence microscopy indicated that GroEL is diffusely distributed, both under normal and stress conditions. Importantly, the present procedure uses a small, fluorescent unnatural amino acid to visualize GroEL in vivo, avoiding...

  5. COMPTEL observations of GRO J1655-40

    Science.gov (United States)

    vanDijk, R.; Bennett, K.; Winkler, C.; Bloemen, H.; Hermsen, W.; Diehl, R.; Schoenfelder, V.; McConnell, M.; Ryan, J.

    1997-01-01

    The transient black hole candidate GRO J1655-40 was shown elsewhere to eject matter at apparently superluminal velocities. The oriented scintillation spectrometer experiment (OSSE) spectra, measured during the X-ray outbursts, are consistent with a single power law up to 600 keV, with no evidence of a high energy cutoff. The detection of the flaring behavior of this source up to 10 MeV was claimed elsewhere. The Compton telescope (COMPTEL) observations in the 0.75 to 30 MeV band of this source are analyzed. The upper limits on the time averaged emission are presented and gamma ray flares are searched for. It is concluded that the COMPTEL data can neither confirm nor refute the claimed detection of a large gamma ray flare from this source. The gamma ray flare search in five years' worth of data did not reveal evidence for the occurrence of strong flares on timescales of two days.

  6. Probing the functional mechanism of Escherichia coli GroEL using circular permutation.

    Directory of Open Access Journals (Sweden)

    Tomohiro Mizobata

    Full Text Available BACKGROUND: The Escherichia coli chaperonin GroEL subunit consists of three domains linked via two hinge regions, and each domain is responsible for a specific role in the functional mechanism. Here, we have used circular permutation to study the structural and functional characteristics of the GroEL subunit. METHODOLOGY/PRINCIPAL FINDINGS: Three soluble, partially active mutants with polypeptide ends relocated into various positions of the apical domain of GroEL were isolated and studied. The basic functional hallmarks of GroEL (ATPase and chaperoning activities were retained in all three mutants. Certain functional characteristics, such as basal ATPase activity and ATPase inhibition by the cochaperonin GroES, differed in the mutants while at the same time, the ability to facilitate the refolding of rhodanese was roughly equal. Stopped-flow fluorescence experiments using a fluorescent variant of the circularly permuted GroEL CP376 revealed that a specific kinetic transition that reflects movements of the apical domain was missing in this mutant. This mutant also displayed several characteristics that suggested that the apical domains were behaving in an uncoordinated fashion. CONCLUSIONS/SIGNIFICANCE: The loss of apical domain coordination and a concomitant decrease in functional ability highlights the importance of certain conformational signals that are relayed through domain interlinks in GroEL. We propose that circular permutation is a very versatile tool to probe chaperonin structure and function.

  7. Single-step Purification of Molecular Chaperone GroEL by Expanded Bed Chromatography

    Institute of Scientific and Technical Information of China (English)

    佟晓冬; 杨征; 董晓燕; 孙彦

    2003-01-01

    Expanded bed adsorption (EBA) is an integrative downstream processing technique for the purification of biological substances directly from unclarified feedstock. In this study, molecular chaperone GroEL, an important protein folding helper both in vivo and in vitro, was purified by the single-step EBA technique from the unclarified homogenate of recombinant E. coli cells. Compared with packed bed adsorption, the EBA technique provided a single-step approach to yield an electrophoretic purity of GroEL. After the homogenate loading and column washing in the expanded bed mode, the GroEL protein was recovered by stepwise salt-gradient elution in packed-bed or expanded-bed modes, respectively. The expanded-bed elution mode was found as efficient as the packed-bed mode in the purification of GroEL from cell disruptate.

  8. Gro/TLE enables embryonic stem cell differentiation by repressing pluripotent gene expression

    DEFF Research Database (Denmark)

    Laing, Adam F; Lowell, Sally; Brickman, Joshua M

    2015-01-01

    Gro/TLE proteins (TLE1-4) are a family of transcriptional corepressors acting downstream of multiple signalling pathways. Several TLEs are expressed in a dynamic manner throughout embryonic development and at high levels in embryonic stem cells (ESCs). Here we find that Gro/TLE is not required...... in ESC for sustaining pluripotency and suppressing differentiation genes, but rather is important for the shutting down of the pluripotency network in differentiation. Consistent with this view, we found that one of the Gro/TLE family, TLE4 is expressed heterogeneously in ESCs in a population...... that corresponds to a Nanog low subset of ESC culture. TLE4 expression is also increased in response to LIF withdrawal and Fgf/Mek/Erk stimulation. To explore the role of Gro/TLE in more detail we generated an allelic series of knockout ESCs of two TLE genes expressed most dynamically in early differentiation, TLE...

  9. The alr-groEL1 operon in Mycobacterium tuberculosis: an interplay of multiple regulatory elements

    Science.gov (United States)

    Bhat, Aadil H.; Pathak, Deepika; Rao, Alka

    2017-01-01

    Threonylcarbamoyladenosine is a universally conserved essential modification of tRNA that ensures translational fidelity in cellular milieu. TsaD, TsaB and TsaE are identified as tRNA-A37-threonylcarbamoyl (t6A)-transferase enzymes that have been reconstituted in vitro, in few bacteria recently. However, transcriptional organization and regulation of these genes are not known in any of these organisms. This study describes the intricate architecture of a complex multicistronic alr-groEL1 operon, harboring essential genes, namely tsaD, tsaB, tsaE, groES, groEL1, and alr (required for cell wall synthesis), and rimI encoding an N-α- acetyltransferase in Mycobacterium tuberculosis. Using northern blotting, RT-PCR and in vivo fluorescence assays, genes alr to groEL1 were found to constitute an ~6.3 kb heptacistronic operon with multiple internal promoters and an I-shaped intrinsic hairpin-like cis-regulatory element. A strong promoter PtsaD within the coding sequence of rimI gene is identified in M. tuberculosis, in addition. The study further proposes an amendment in the known bicistronic groESL1 operon annotation by providing evidence that groESL1 is co-transcribed as sub-operon of alr-groEL1 operon. The architecture of alr-groEL1 operon, conservation of the genetic context and a mosaic transcriptional profile displayed under various stress conditions convincingly suggest the involvement of this operon in stress adaptation in M. tuberculosis. PMID:28256563

  10. Clon of GroEL gene and construction of eukaryotic expression vector in myobacterium tuberclosis%结核分枝杆菌GroEL基因的克隆及真核表达质粒的构建

    Institute of Scientific and Technical Information of China (English)

    付风云; 张卫英

    2009-01-01

    目的 克隆结核分枝杆菌基因组携带的两组GroEL基因,即GroEL1和GroEL2,它们分别编码热休克蛋白HSP60(cpn60.1)和HSP65(cpn60.2),并构建真核表达质粒.方法 用带有EcoR I和Xba I酶切位点的特异性引物,从结核杆菌H37Rv株中PCR扩增两组GroEL基因,构建重组真核表达质粒pCDNA3.1-GroEL1和pCDNA3.1-GroEL2,并进行酶切和测序鉴定.结果 成功克隆了结核分枝杆菌两组GroEL基因,酶切鉴定pCDNA3.1-GroEL1和pCDNA3.1-GroEL2构建成功,经DNA测序证实,载体上插入的序列与GenBank公布的序列一致.结论 获得了结核分枝杆菌GroEL1和GroEL2基因,成功地构建了含GroEL1和GroEL2基因的真核表达质粒,为研究其作为核酸疫苗在免疫学方面作用提供材料.

  11. BeppoSAX observations of GRO J1744-28

    CERN Document Server

    Doroshenko, R; Doroshenko, V; Suleimanov, V; Piraino, S

    2015-01-01

    We present an analysis of BeppoSAX observations of the unique transient bursting X-ray pulsar GRO J1744-28. The observations took place in March 1997 during the decay phase of the outburst. We find that the persistent broadband X-ray continuum of the source is consistent with a cutoff power law typical for the accreting pulsars. We also detect the fluorescence iron line at 6.7 keV and an absorption feature at ~4.5 keV, which we interpret as a cyclotron line. The corresponding magnetic field strength in the line forming region is ~3.7 x 10^11 G. Neither line is detected in the spectra of the bursts. However, additional soft thermal component with kT ~2 keV was required to describe the burst spectrum. We briefly discuss the nature of this component and argue that among other possibilities it might be connected with thermonuclear flashes at the neutron star surface which accompany the accretion-powered bursts in the source.

  12. Gro2mat: a package to efficiently read gromacs output in MATLAB.

    Science.gov (United States)

    Dien, Hung; Deane, Charlotte M; Knapp, Bernhard

    2014-07-30

    Molecular dynamics (MD) simulations are a state-of-the-art computational method used to investigate molecular interactions at atomic scale. Interaction processes out of experimental reach can be monitored using MD software, such as Gromacs. Here, we present the gro2mat package that allows fast and easy access to Gromacs output files from Matlab. Gro2mat enables direct parsing of the most common Gromacs output formats including the binary xtc-format. No openly available Matlab parser currently exists for this format. The xtc reader is orders of magnitudes faster than other available pdb/ascii workarounds. Gro2mat is especially useful for scientists with an interest in quick prototyping of new mathematical and statistical approaches for Gromacs trajectory analyses. © 2014 Wiley Periodicals, Inc.

  13. Análise funcional das proteínas HrcA, GroES/GroEL e DnaK/DnaJ em Caulobacter crescentus

    OpenAIRE

    Michelle Fernanda Susin

    2005-01-01

    O operon groESL de C. crescentus apresenta dupla regulação. A indução deste operon por choque térmico é dependente do fator sigma de choque térmico σ32. A temperaturas fisiológicas, a expressão de groESL apresenta regulação temporal durante o ciclo celular da bactéria e o controle envolve a proteína repressora HrcA e o elemento CIRCE (controlling inverted repeat of chaperonin expression). Para estudar a atividade da proteína repressora in vitro, produzimos e purificamos de E. coli a Hrc...

  14. Stereoscopic observations of hard x ray sources in solar flares made with GRO and other spacecraft

    Science.gov (United States)

    Kane, S. R.; Hurley, K.; Mctiernan, J. M.; Laros, J. G.

    1992-01-01

    Since the launch of the Gamma Ray Observatory (GRO) in Apr. 1991, the Burst and Transient Source Experiment (BATSE) instrument on GRO has recorded a large number of solar flares. Some of these flares have also been observed by the Gamma-Ray Burst Detector on the Pioneer Venus Orbiter (PVO) and/or by the Solar X-Ray/Cosmic Gamma-Ray Burst Experiment on the Ulysses spacecraft. A preliminary list of common flares observed during the period May-Jun. 1991 is presented and the possible joint studies are indicated.

  15. Bibliothekarische Berufsethik in der Praxis – Ergebnisse eines Studienprojekts aus Deutschland und Großbritannien

    Directory of Open Access Journals (Sweden)

    Jens Boye

    2011-10-01

    Full Text Available Sowohl in Deutschland als auch in Großbritannien existieren seit einigen Jahren Grundsatzpapiere zur Berufsethik im Informations- und Bibliotheksbereich. Neben der bloßen Existenz derartiger Kodizes stellt sich die Frage, welche Relevanz diese Dokumente in der beruflichen Praxis haben. Dieser Frage wurde im Rahmen einer Team-Projektarbeit an der FH Köln nachgegangen. Mithilfe eines standardisierten Fragebogens wurden deutsche Bibliotheksleitungen zu der Thematik befragt. In Großbritannien wurde ein Interview mit Paul Sturges geführt, darüber hinaus erfolgte eine Analyse der Online-Angebote von CILIP, insbesondere des Information Ethics Blog.

  16. Porphyromonas gingivalis GroEL induces osteoclastogenesis of periodontal ligament cells and enhances alveolar bone resorption in rats.

    Directory of Open Access Journals (Sweden)

    Feng-Yen Lin

    Full Text Available Porphyromonas gingivalis is a major periodontal pathogen that contains a variety of virulence factors. The antibody titer to P. gingivalis GroEL, a homologue of HSP60, is significantly higher in periodontitis patients than in healthy control subjects, suggesting that P. gingivalis GroEL is a potential stimulator of periodontal disease. However, the specific role of GroEL in periodontal disease remains unclear. Here, we investigated the effect of P. gingivalis GroEL on human periodontal ligament (PDL cells in vitro, as well as its effect on alveolar bone resorption in rats in vivo. First, we found that stimulation of PDL cells with recombinant GroEL increased the secretion of the bone resorption-associated cytokines interleukin (IL-6 and IL-8, potentially via NF-κB activation. Furthermore, GroEL could effectively stimulate PDL cell migration, possibly through activation of integrin α1 and α2 mRNA expression as well as cytoskeletal reorganization. Additionally, GroEL may be involved in osteoclastogenesis via receptor activator of nuclear factor κ-B ligand (RANKL activation and alkaline phosphatase (ALP mRNA inhibition in PDL cells. Finally, we inoculated GroEL into rat gingiva, and the results of microcomputed tomography (micro-CT and histomorphometric assays indicated that the administration of GroEL significantly increased inflammation and bone loss. In conclusion, P. gingivalis GroEL may act as a potent virulence factor, contributing to osteoclastogenesis of PDL cells and resulting in periodontal disease with alveolar bone resorption.

  17. GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function.

    Directory of Open Access Journals (Sweden)

    Yi-Wen Lin

    Full Text Available The number and function of endothelial progenitor cells (EPCs are sensitive to hyperglycemia, hypertension, and smoking in humans, which are also associated with the development of atherosclerosis. GroEL1 from Chlamydia pneumoniae has been found in atherosclerotic lesions and is related to atherosclerotic pathogenesis. However, the actual effects of GroEL1 on EPC function are unclear. In this study, we investigate the EPC function in GroEL1-administered hind limb-ischemic C57BL/B6 and C57BL/10ScNJ (a toll-like receptor 4 (TLR4 mutation mice and human EPCs. In mice, laser Doppler imaging, flow cytometry, and immunohistochemistry were used to evaluate the degree of neo-vasculogenesis, circulating level of EPCs, and expression of CD34, vWF, and endothelial nitric oxide synthase (eNOS in vessels. Blood flow in the ischemic limb was significantly impaired in C57BL/B6 but not C57BL/10ScNJ mice treated with GroEL1. Circulating EPCs were also decreased after GroEL1 administration in C57BL/B6 mice. Additionally, GroEL1 inhibited the expression of CD34 and eNOS in C57BL/B6 ischemic muscle. In vitro, GroEL1 impaired the capacity of differentiation, mobilization, tube formation, and migration of EPCs. GroEL1 increased senescence, which was mediated by caspases, p38 MAPK, and ERK1/2 signaling in EPCs. Furthermore, GroEL1 decreased integrin and E-selectin expression and induced inflammatory responses in EPCs. In conclusion, these findings suggest that TLR4 and impaired NO-related mechanisms could contribute to the reduced number and functional activity of EPCs in the presence of GroEL1 from C. pneumoniae.

  18. Further observations of GRO J1750-27 (AXJ1749.1-2639) with INTEGRAL

    DEFF Research Database (Denmark)

    Brandt, Søren Kristian; Shaw, S.; Hill, A.;

    2008-01-01

    The transient accreting X-ray pulsar GRO J1750-27 (AX J1749.1-2639), which became active end of January 2008 (ATel #1376), has been repeatedly observed by the INTEGRAL Galactic Bulge monitoring program since mid February (ATel #1385) on 11, 20 and 23 Feb. 2008. During the three observations...

  19. Rapidly evolving circularly polarized emission during the 1994 outburst of GRO J1655-40

    NARCIS (Netherlands)

    Macquart, JP; Wu, K; Sault, RJ; Hannikainen, DC

    2002-01-01

    We report the detection of circular polarization during the 1994 outburst of the Galactic microquasar GRO J1655-40. The circular polarization is clearly detected at 1.4 and 2.4 GHz, but not at 4.8 and 8.4 GHz, where its magnitude never exceeds 5 mJy. Both the sign and magnitude of the circular

  20. Positive selection in the leucine-rich repeat domain of Gro1 genes in Solanum species

    Indian Academy of Sciences (India)

    Valentino Ruggieri; Angelina Nunziata; Amalia Barone

    2014-12-01

    In pathogen resistant plants, solvent-exposed residues in the leucine-rich repeat (LRR) proteins are thought to mediate resistance by recognizing plant pathogen elicitors. In potato, the gene Gro1-4 confers resistance to Globodera rostochiensis. The investigation of variablity in different copies of this gene represents a good model for the verification of positive selection mechanisms. Two datasets of Gro1 LRR sequences were constructed, one derived from the Gro1-4 gene, belonging to different cultivated and wild Solanum species, and the other belonging to paralogues of a resistant genotype. Analysis of non-synonymous to synonymous substitution rates $(K_{a}/K_{s})$ highlighted 14 and six amino acids with $K_{a}/K_{s} \\gt 1$ in orthologue and paralogue datasets, respectively. Selection analysis revealed that the leucine-rich regions accumulate variability in a very specific way, and we found that some combinations of amino acids in these sites might be involved in pathogen recognition. The results confirm previous studies on positive selection in the LRR domain of R protein in Arabidopsis and other model plants and extend these to wild Solanum species. Moreover, positively selected sites in the Gro1 LRR domain show that coevolution mainly occurred in two regions on the internal surface of the three-dimensional horseshoe structure of the domain, albeit with different evolutionary forces between paralogues and orthologues.

  1. The Accretion Powered Spin-up of GRO 1750–27

    DEFF Research Database (Denmark)

    Kretschmar, P.; Shaw, S.; Hill, A. B.

    2009-01-01

    The transient Be X-ray pulsar GRO J1750-27 was originally detected in 1995 by CGRO/BATSE during a giant outburst. After a long period of quiescence the source was detected in another outburst early 2008. Following this outburst with hard X-ray data from INTEGRAL and Swift, the orbital parameters ...

  2. The accretion powered spin-up of GRO J1750-27

    DEFF Research Database (Denmark)

    Shaw, S.E.; Hill, A.B.; Kuulkers, E.

    2009-01-01

    ), respectively. The model is then used to compare the time-resolved variation of the X-ray flux and intrinsic spin-up against the accretion torque model of Ghosh & Lamb; this finds that GRO J1750-27 is likely located 12-22 kpc distant and that the surface magnetic field of the neutron star is similar to 2 x 10...

  3. Computational Approaches for Mining GRO-Seq Data to Identify and Characterize Active Enhancers.

    Science.gov (United States)

    Nagari, Anusha; Murakami, Shino; Malladi, Venkat S; Kraus, W Lee

    2017-01-01

    Transcriptional enhancers are DNA regulatory elements that are bound by transcription factors and act to positively regulate the expression of nearby or distally located target genes. Enhancers have many features that have been discovered using genomic analyses. Recent studies have shown that active enhancers recruit RNA polymerase II (Pol II) and are transcribed, producing enhancer RNAs (eRNAs). GRO-seq, a method for identifying the location and orientation of all actively transcribing RNA polymerases across the genome, is a powerful approach for monitoring nascent enhancer transcription. Furthermore, the unique pattern of enhancer transcription can be used to identify enhancers in the absence of any information about the underlying transcription factors. Here, we describe the computational approaches required to identify and analyze active enhancers using GRO-seq data, including data pre-processing, alignment, and transcript calling. In addition, we describe protocols and computational pipelines for mining GRO-seq data to identify active enhancers, as well as known transcription factor binding sites that are transcribed. Furthermore, we discuss approaches for integrating GRO-seq-based enhancer data with other genomic data, including target gene expression and function. Finally, we describe molecular biology assays that can be used to confirm and explore further the function of enhancers that have been identified using genomic assays. Together, these approaches should allow the user to identify and explore the features and biological functions of new cell type-specific enhancers.

  4. Over expression of GroESL in Cupriavidus necator for heterotrophic and autotrophic isopropanol production.

    Science.gov (United States)

    Marc, Jillian; Grousseau, Estelle; Lombard, Eric; Sinskey, Anthony J; Gorret, Nathalie; Guillouet, Stéphane E

    2017-07-01

    We previously reported a metabolic engineering strategy to develop an isopropanol producing strain of Cupriavidus necator leading to production of 3.4gL(-1) isopropanol. In order to reach higher titers, isopropanol toxicity to the cells has to be considered. A toxic effect of isopropanol on the growth of C. necator has been indeed observed above a critical value of 15gL(-1). GroESL chaperones were first searched and identified in the genome of C. necator. Native groEL and groES genes from C. necator were over-expressed in a strain deleted for PHA synthesis. We demonstrated that over-expressing groESL genes led to a better tolerance of the strain towards exogenous isopropanol. GroESL genes were then over-expressed within the best engineered isopropanol producing strain. A final isopropanol concentration of 9.8gL(-1) was achieved in fed-batch culture on fructose as the sole carbon source (equivalent to 16gL(-1) after taking into account evaporation). Cell viability was slightly improved by the chaperone over-expression, particularly at the end of the fermentation when the isopropanol concentration was the highest. Moreover, the strain over-expressing the chaperones showed higher enzyme activity levels of the 2 heterologous enzymes (acetoacetate carboxylase and alcohol dehydrogenase) of the isopropanol synthetic operon, translating to a higher specific production rate of isopropanol at the expense of the specific production rate of acetone. Over-expressing the native chaperones led to a 9-18% increase in the isopropanol yield on fructose. Copyright © 2017 International Metabolic Engineering Society. All rights reserved.

  5. The morphogenesis of herpes simplex virus type 1 in infected parental mouse L fibroblasts and mutant gro29 cells

    DEFF Research Database (Denmark)

    Jensen, Helle Lone; Norrild, Bodil

    2003-01-01

    Mutants of cell lines and viruses are important biological tools. The pathway of herpesvirus particle maturation and egress are contentious issues. The mutant gro29 line of mouse L cells is defective for egress of herpes simplex virus type 1 (HSV-1) virions, and a candidate for studies of virus......-cell interactions. The properties of uninfected and HSV-1-infected L fibroblasts and gro29 cells investigated by protein assay, immunoblot, titration assay, immunofluorescence light microscopy and immunogold cryosection electron microscopy are reported. The ultrastructure of both HSV-1-infected L and gro29 cells...

  6. Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES

    Directory of Open Access Journals (Sweden)

    Jariyachawalid Kanidtha

    2012-04-01

    Full Text Available Abstract Background D-phenylglycine aminotransferase (D-PhgAT of Pseudomonas stutzeri ST-201 catalyzes the reversible stereo-inverting transamination potentially useful in the application for synthesis of D-phenylglycine and D-4-hydroxyphenylglycine using L-glutamate as a low cost amino donor substrate in one single step. The enzyme is a relatively hydrophobic homodimeric intracellular protein difficult to express in the soluble functionally active form. Over-expression of the dpgA gene in E. coli resulted in the majority of the D-PhgAT aggregated into insoluble inclusion bodies that failed to be re-natured. Expression in Pichia pastoris was explored as an alternative route for high level production of the D-PhgAT. Results Intracellular expression of the codon-optimized synthetic dpgA gene under the PAOX1 promoter in P. pastoris resulted in inactive D-PhgAT associated with insoluble cellular fraction and very low level of D-PhgAT activity in the soluble fraction. Manipulation of culture conditions such as addition of sorbitol to induce intracellular accumulation of osmolytes, addition of benzyl alcohol to induce chaperone expression, or lowering incubation temperature to slow down protein expression and folding rates all failed to increase the active D-PhgAT yield. Co-expression of E. coli chaperonins GroEL-GroES with the D-PhgAT dramatically improved the soluble active enzyme production. Increasing gene dosage of both the dpgA and those of the chaperones further increased functional D-PhgAT yield up to 14400-fold higher than when the dpgA was expressed alone. Optimization of cultivation condition further increased D-PhgAT activity yield from the best co-expressing strain by 1.2-fold. Conclusions This is the first report on the use of bacterial chaperones co-expressions to enhance functional intracellular expression of bacterial enzyme in P. pastoris. Only two bacterial chaperone genes groEL and groES were sufficient for dramatic enhancement of

  7. 利用膨胀床吸附技术单步纯化分子伴侣—GroEL%Single-step Purification of Molecular Chaperone GroEL by Expanded Bed Chromatography

    Institute of Scientific and Technical Information of China (English)

    佟晓冬; 杨征; 董晓燕; 孙彦

    2003-01-01

    Expanded bed adsorption (EBA) is an integrative downstream processing technique for the purificationof biological substances directly from unclarified feedstock. In this study, molecular chaperone GroEL, an importantprotein folding helper both in vivo and in vitro, was purified by the single-step EBA technique from the unclarifiedhomogenate of recombinant E. coli cells. Compared with packed bed adsorption, the EBA technique provideda single-step approach to yield an electrophoretic purity of GroEL. After the homogenate loading and columnwashing in the expanded bed mode, the GroEL protein was recovered by stepwise salt-gradient elution in packed-bed or expanded-bed modes, respectively. The expanded-bed elution mode was found as efficient as the packed-bedmode in the purification of GroEL from cell disruptate.

  8. Induction of heat shock proteins DnaK, GroEL, and GroES by salt stress in Lactococcus lactis

    DEFF Research Database (Denmark)

    Kilstrup, Mogens; Jacobsen, Susanne; Hammer, Karin;

    1997-01-01

    The bacterium Lactococcus lactis has become a model organism in studies of growth physiology and membrane transport, as a result of its simple fermentative metabolism. It is also used as a model for studying the importance of specific genes and functions during lie in excess nutrients, by compari......The bacterium Lactococcus lactis has become a model organism in studies of growth physiology and membrane transport, as a result of its simple fermentative metabolism. It is also used as a model for studying the importance of specific genes and functions during lie in excess nutrients......, by comparison of prototrophic wild-type strains and auxotrophic domesticated (daily) strains. In a study of the capacity of domesticated strains to perform directed responses toward various stress conditions, we have analyzed the heat and salt stress response in the established L,. lactis subsp. cremoris...... laboratory strain MG1363, which was originally derived from a dairy strain, After two-dimensional separation of proteins, the DnaK, GroEL, and GroES heat shock proteins, the HrcA (Orf1) heat shack repressor, and the glycolytic enzymes pyruvate kinase, glyceral-dehyde-3-phosphate dehydrogenase...

  9. Perturbation-based Markovian transmission model for probing allosteric dynamics of large macromolecular assembling: a study of GroEL-GroES.

    Directory of Open Access Journals (Sweden)

    Hsiao-Mei Lu

    2009-10-01

    Full Text Available Large macromolecular assemblies are often important for biological processes in cells. Allosteric communications between different parts of these molecular machines play critical roles in cellular signaling. Although studies of the topology and fluctuation dynamics of coarse-grained residue networks can yield important insights, they do not provide characterization of the time-dependent dynamic behavior of these macromolecular assemblies. Here we develop a novel approach called Perturbation-based Markovian Transmission (PMT model to study globally the dynamic responses of the macromolecular assemblies. By monitoring simultaneous responses of all residues (>8,000 across many (>6 decades of time spanning from the initial perturbation until reaching equilibrium using a Krylov subspace projection method, we show that this approach can yield rich information. With criteria based on quantitative measurements of relaxation half-time, flow amplitude change, and oscillation dynamics, this approach can identify pivot residues that are important for macromolecular movement, messenger residues that are key to signal mediating, and anchor residues important for binding interactions. Based on a detailed analysis of the GroEL-GroES chaperone system, we found that our predictions have an accuracy of 71-84% judged by independent experimental studies reported in the literature. This approach is general and can be applied to other large macromolecular machineries such as the virus capsid and ribosomal complex.

  10. Luminosity dependent accretion state change in GRO J1008–57

    Directory of Open Access Journals (Sweden)

    Kühnel Matthias

    2014-01-01

    Full Text Available In a former publication, we have analyzed the transient neutron star X-ray binary GRO J1008–57 using all available RXTE-, Swift-, and Suzaku-data. As we have found, the source’s spectral components, i.e., a power-law with high exponential cutoff and a black-body, are strongly correlated with the hard X-ray flux (15–50 keV. We update the analytical description of these dependence, including a change in the photon index behaviour from a flat to a logarithmic function. The flux, where the change occurs, is consistent with the onset of the black-body emission. Thus, a change of the accretion state always occurs in GRO J1008–57 at a particular flux level.

  11. Adsorption of a model protein, the GroEL chaperonin, on surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Leung, Carl; Palmer, Richard E [Nanoscale Physics Research Laboratory, School of Physics and Astronomy, University of Birmingham, Edgbaston, Birmingham B15 2TT (United Kingdom)], E-mail: carl.leung@kcl.ac.uk

    2008-09-03

    Understanding and controlling protein adsorption on surfaces is fundamental to many biological processes ranging from cell adhesion to the fabrication of protein biochips. In general, proteins need to retain their 3D conformation to perform their intended functions. However, when they are presented with a solid surface, complex interactions ranging from weak non-covalent binding to strong covalent bonding may occur, which can potentially induce conformational changes within the adsorbed protein. To investigate the surface adsorption process and its effects on a model protein, the chaperonin GroEL, we have applied contact mode atomic force microscopy, in buffer solution to probe the interactions between single proteins and surfaces in real space. We will discuss the adsorption of GroEL molecules on planar surfaces (mica, graphite and gold) and specifically tailored nanostructured surfaces, which present structural features on the size scale of individual biological molecules. (topical review)

  12. Thermonuclear Burning on the Accreting X-Ray Pulsar GRO J1744-28

    CERN Document Server

    Bildsten, L; Bildsten, Lars; Brown, Edward F.

    1996-01-01

    We investigate the thermal stability of nuclear burning on the accreting X-ray pulsar GRO J1744-28. The neutron star's dipolar magnetic field is 50 years. We also discuss the nature of the binary and point out that a velocity measurement of the stellar companion (most likely a Roche-lobe filling giant with m_K>17) will constrain the neutron star mass.

  13. INTEGRAL/XMM views on the MeV source GRO J1411-64

    CERN Document Server

    Torres, D F; Reimer, O; Barcons, X; Corral, A; Bosch-Ramon, V; Paredes, J M; Romero, G E; Qu, J L; Collmar, W; Schönfelder, V; Butt, Y; Torres, Diego F.; Zhang, Shu; Reimer, Olaf; Barcons, Xavier; Corral, Amalia; Bosch-Ramon, Valent\\'{\\i}; Paredes, Josep M.; Romero, Gustavo E.; Qu, Jin Lu; Collmar, Werner; Sch\\"onfelder, Volker; Butt, Yousaf

    2006-01-01

    The COMPTEL unidentified source GRO J 1411-64 was observed by INTEGRAL and XMM-Newton in 2005. The Circinus Galaxy is the only source detected within the 4$\\sigma$ location error of GRO J1411-64, but in here excluded as the possible counterpart. At soft X-rays, 22 reliable and statistically significant sources (likelihood $> 10$) were extracted and analyzed from XMM-Newton data. Only one of these sources, XMMU J141255.6 -635932, is spectrally compatible with GRO J1411-64 although the fact the soft X-ray observations do not cover the full extent of the COMPTEL source position uncertainty make an association hard to quantify and thus risky. At the best location of the source, detections at hard X-rays show only upper limits, which, together with MeV results obtained by COMPTEL suggest the existence of a peak in power output located somewhere between 300-700 keV for the so-called low state. Such a spectrum resembles those in blazars or microquasars, and might suggest at work by a similar scenario. However, an an...

  14. Temporal Variations And Spectral Properties of Be/X-ray Pulsar GRO J1008-57 Studied by INTEGRAL

    CERN Document Server

    Wang, Wei

    2013-01-01

    Spin period variations and hard X-ray spectral properties of Be/X-ray pulsar GRO J1008-57 are studied with INTEGRAL observations. The pulsation periods at 93.66 s in 2004 and at 93.73 s in 2009 are determined. Pulse profiles of GRO J1008-57 during outbursts are energy dependent: double-peak profile in soft bands of 3-7 keV and single-peak profile in hard X-rays above 7 keV. GRO J1008-57 has undergone a spin-down trend from 1993-2009 with a rate of 4.1x10^-5 s day^-1, and might transfer into a spin-up trend after 2009. The spectra from 3-100 keV of GRO J1008-57 during outbursts are fitted with photon index of 1.4 and cutoff energies of 23-29 keV. We find a relatively soft spectrum in early phase of the 2009 outburst with cutoff energy 13 keV. Above a hard X-ray flux of 10^-9 erg cm^-2 s^-1, the spectra of GRO J1008-57 during outbursts need an enhanced hydrogen absorption of column density 6x10^22 cm^-2. The observed dip-like pulse profile of GRO J1008-57 in soft X-ray bands should be caused by this intrinsic a...

  15. Intrinsic unfoldase/foldase activity of the chaperonin GroEL directly demonstrated using multinuclear relaxation-based NMR

    Science.gov (United States)

    Libich, David S.; Tugarinov, Vitali; Clore, G. Marius

    2015-01-01

    The prototypical chaperonin GroEL assists protein folding through an ATP-dependent encapsulation mechanism. The details of how GroEL folds proteins remain elusive, particularly because encapsulation is not an absolute requirement for successful re/folding. Here we make use of a metastable model protein substrate, comprising a triple mutant of Fyn SH3, to directly demonstrate, by simultaneous analysis of three complementary NMR-based relaxation experiments (lifetime line broadening, dark state exchange saturation transfer, and Carr–Purcell–Meinboom–Gill relaxation dispersion), that apo GroEL accelerates the overall interconversion rate between the native state and a well-defined folding intermediate by about 20-fold, under conditions where the “invisible” GroEL-bound states have occupancies below 1%. This is largely achieved through a 500-fold acceleration in the folded-to-intermediate transition of the protein substrate. Catalysis is modulated by a kinetic deuterium isotope effect that reduces the overall interconversion rate between the GroEL-bound species by about 3-fold, indicative of a significant hydrophobic contribution. The location of the GroEL binding site on the folding intermediate, mapped from 15N, 1HN, and 13Cmethyl relaxation dispersion experiments, is composed of a prominent, surface-exposed hydrophobic patch. PMID:26124125

  16. Increased expression and purification of soluble iron-regulatory protein 1 from Escherichia coli co-expressing chaperonins GroES and GroEL

    Directory of Open Access Journals (Sweden)

    H. Carvalho

    2008-04-01

    Full Text Available Iron is an essential metal for all living organisms. However, iron homeostasis needs to be tightly controlled since iron can mediate the production of reactive oxygen species, which can damage cell components and compromise the integrity and/or cause DNA mutations, ultimately leading to cancer. In eukaryotes, iron-regulatory protein 1 (IRP1 plays a central role in the control of intracellular iron homeostasis. This occurs by interaction of IRP1 with iron-responsive element regions at 5' of ferritin mRNA and 3' of transferrin mRNA which, respectively, represses translation and increases mRNA stability. We have expressed IRP1 using the plasmid pT7-His-hIRP1, which codifies for human IRP1 attached to an NH2-terminal 6-His tag. IRP1 was expressed in Escherichia coli using the strategy of co-expressing chaperonins GroES and GroEL, in order to circumvent inclusion body formation and increase the yield of soluble protein. The protein co-expressed with these chaperonins was obtained mostly in the soluble form, which greatly increased the efficiency of protein purification. Metal affinity and FPLC ion exchange chromatography were used in order to obtain highly purified IRP1. Purified protein was biologically active, as assessed by electrophoretic mobility shift assay, and could be converted to the cytoplasmic aconitase form. These results corroborate previous studies, which suggest the use of folding catalysts as a powerful strategy to increase protein solubility when expressing heterologous proteins in E. coli.

  17. GroEL to DnaK chaperone network behind the stability modulation of σ(32) at physiological temperature in Escherichia coli.

    Science.gov (United States)

    Patra, Monobesh; Roy, Sourav Singha; Dasgupta, Rakhi; Basu, Tarakdas

    2015-12-21

    The stability of heat-shock transcription factor σ(32) in Escherichia coli has long been known to be modulated only by its own transcribed chaperone DnaK. Very few reports suggest a role for another heat-shock chaperone, GroEL, for maintenance of cellular σ(32) level. The present study demonstrates in vivo physical association between GroEL and σ(32) in E. coli at physiological temperature. This study further reveals that neither DnaK nor GroEL singly can modulate σ(32) stability in vivo; there is an ordered network between them, where GroEL acts upstream of DnaK.

  18. Effects of the chaperonin GroE on the refolding of tryptophanase from Escherichia coli. Refolding is enhanced in the presence of ADP.

    Science.gov (United States)

    Mizobata, T; Akiyama, Y; Ito, K; Yumoto, N; Kawata, Y

    1992-09-01

    The refolding of the tetrameric enzyme tryptophanase was facilitated by the chaperonin GroE. Maximum refolding yield of tryptophanase molecules (about 80%) was attained in the presence of a 15-fold excess of GroE 21-mer over tryptophanase monomer. The GroEL subunit was required for this improvement in refolding yield, whereas the GroES subunit was not. Light scattering experiments of the refolding reaction revealed that GroE bound to tryptophanase folding intermediates and suppressed their aggregation. The presence of ATP was required for the efficient dissociation of tryptophanase from GroEL. However, our experiments indicated that tryptophanase dissociated readily from GroEL in the presence of not only ATP, but also in the presence of non-hydrolyzable ATP analogues such as ATP gamma S (adenosine 5'-O-(3-thiotriphosphate)) and AMP-PNP (adenyl-5'-yl imidodiphosphate) as well. Surprisingly, the release of tryptophanase from GroEL was facilitated in the presence of ADP as well. We concluded that the binding of nucleotides such as ATP and ADP changed the conformation of GroEL and facilitated the dissociation of tryptophanase molecules. The conformation formed in the presence of ADP was distinct from the conformation formed in the presence of ATP, as shown by the selective dissociation of various folding proteins from the two conformations.

  19. An insecticidal GroEL protein with chitin binding activity from Xenorhabdus nematophila.

    Science.gov (United States)

    Joshi, Mohan Chandra; Sharma, Animesh; Kant, Sashi; Birah, Ajanta; Gupta, Gorakh Prasad; Khan, Sharik R; Bhatnagar, Rakesh; Banerjee, Nirupama

    2008-10-17

    Xenorhabdus nematophila secretes insecticidal proteins to kill its larval prey. We have isolated an approximately 58-kDa GroEL homolog, secreted in the culture medium through outer membrane vesicles. The protein was orally insecticidal to the major crop pest Helicoverpa armigera with an LC50 of approximately 3.6 microg/g diet. For optimal insecticidal activity all three domains of the protein, apical, intermediate, and equatorial, were necessary. The apical domain alone was able to bind to the larval gut membranes and manifest low level insecticidal activity. At equimolar concentrations, the apical domain contained approximately one-third and the apical-intermediate domain approximately one-half bioactivity of that of the full-length protein. Interaction of the protein with the larval gut membrane was specifically inhibited by N-acetylglucosamine and chito-oligosaccharides. Treatment of the larval gut membranes with chitinase abolished protein binding. Based on the three-dimensional structural model, mutational analysis demonstrated that surface-exposed residues Thr-347 and Ser-356 in the apical domain were crucial for both binding to the gut epithelium and insecticidal activity. Double mutant T347A,S356A was 80% less toxic (p activity with Kd approximately 0.64 microm and Bmax approximately 4.68 micromol/g chitin. The variation in chitin binding activity of the mutant proteins was in good agreement with membrane binding characteristics and insecticidal activity. The less toxic double mutant XnGroEL showed an approximately 8-fold increase of Kd in chitin binding assay. Our results demonstrate that X. nematophila secretes an insecticidal GroEL protein with chitin binding activity.

  20. INTEGRAL and XMM-Newton observations towards the unidentified MeV source GRO J1411-64

    CERN Document Server

    Torres, D F; Reimer, O; Barcons, X; Corral, A; Bosch-Ramon, V; Paredes, J M; Romero, G E; Qu, J L; Collmar, W; Schönfelder, V; Butt, Y; Torres, Diego F.; Zhang, Shu; Reimer, Olaf; Barcons, Xavier; Corral, Amalia; Bosch-Ramon, Valenti; Paredes, Josep M.; Romero, Gustavo E.; Qu, Jin Lu; Collmar, Werner; Butt, Yousaf

    2006-01-01

    The COMPTEL unidentified source GRO J1411-64 was observed by INTEGRAL, and its central part, also by XMM-Newton. The data analysis shows no hint for new detections at hard X-rays. The upper limits in flux herein presented constrain the energy spectrum of whatever was producing GRO J1411-64, imposing, in the framework of earlier COMPTEL observations, the existence of a peak in power output located somewhere between 300-700 keV for the so-called low state. The Circinus Galaxy is the only source detected within the 4$\\sigma$ location error of GRO J1411-64, but can be safely excluded as the possible counterpart: the extrapolation of the energy spectrum is well below the one for GRO J1411-64 at MeV energies. 22 significant sources (likelihood $> 10$) were extracted and analyzed from XMM-Newton data. Only one of these sources, XMMU J141255.6-635932, is spectrally compatible with GRO J1411-64 although the fact the soft X-ray observations do not cover the full extent of the COMPTEL source position uncertainty make an...

  1. Eine CarSharing-Zielgruppenanalyse der großen SrV-Vergleichsstädte

    OpenAIRE

    Hahn, Christoph

    2015-01-01

    Due to enormous growth rates during the last couple of years CarSharing has become an interesting field of research. This Master Thesis focuses on the analysis of CarSharing users. For this purpose more than 37.000 participants of the "Große SrV-Vergleichsstädte"-Survey were taken into account. After a short literature review and some general information about the statistical methods, the thesis tries to work out some major characteristics of the typical CarSharing user. It will be shown, tha...

  2. NuSTAR Observations of the Be/X-ray Binary GRO J1008-57 in Outburst

    Science.gov (United States)

    Bellm, Eric; Fuerst, F.; Pottschmidt, K.; Wilms, J.; Boggs, S. E.; Christensen, F.; Craig, W. W.; Hailey, C. J.; Harrison, F.; Stern, D.; Tomsick, J.; Zhang, W.; NuSTAR Science Team

    2013-04-01

    GRO J1008-57 is an HMXB that exhibits regular outbursts due to orbitally-driven accretion onto the neutron star from the Be companion. Spectral analysis of previous bursts have suggested the existence of a cyclotron resonant scattering feature at 88 keV, which, if confirmed as the fundamental line, would indicate the presence of one of the most highly-magnetized accreting neutron stars. In November 2012, GRO J1008 produced a Type II ("giant") burst with peak flux near 1 Crab. NuSTAR observed the outburst for 12 ksec near the peak of emission. We present spectral and timing analysis of GRO J1008 over NuSTAR's 3-79 keV energy band. Considering that the putative 88 keV line could be a harmonic rather than the fundamental, we search for cyclotron lines in the NuSTAR band and discuss implications for models of the system's magnetic field.

  3. Confirmation of a high magnetic field in GRO J1008–57

    Energy Technology Data Exchange (ETDEWEB)

    Bellm, Eric C.; Fürst, Felix; Harrison, Fiona A.; Walton, Dominic J. [Cahill Center for Astronomy and Astrophysics, California Institute of Technology, Pasadena, CA 91125 (United States); Pottschmidt, Katja [Center for Space Science and Technology, University of Maryland Baltimore County, Baltimore, MD 21250 (United States); Tomsick, John A.; Boggs, Steven E.; Craig, William W. [Space Sciences Laboratory, University of California, Berkeley, CA 94720 (United States); Chakrabarty, Deepto [Kavli Institute for Astrophysics and Space Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Christensen, Finn E. [DTU Space-National Space Institute, Technical University of Denmark, Elektrovej 327, DK-2800 Lyngby (Denmark); Hailey, Charles J. [Columbia Astrophysics Laboratory, Columbia University, New York, NY 10027 (United States); Stern, Daniel [Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109 (United States); Wilms, Jörn [Dr. Karl-Remeis-Sternwarte and ECAP, Sternwartstr. 7, D-96049 Bamberg (Germany); Zhang, William W., E-mail: ebellm@caltech.edu [NASA Goddard Space Flight Center, Greenbelt, MD 20771 (United States)

    2014-09-10

    GRO J1008–57 is a high-mass X-ray binary for which several claims of a cyclotron resonance scattering feature near 80 keV have been reported. We use NuSTAR, Suzaku, and Swift data from its giant outburst of 2012 November to confirm the existence of the 80 keV feature and perform the most sensitive search to date for cyclotron scattering features at lower energies. We find evidence for a 78{sub −2}{sup +3} keV line in the NuSTAR and Suzaku data at >4σ significance, confirming the detection using Suzaku alone by Yamamoto et al. A search of both the phase-averaged and phase-resolved data rules out a fundamental at lower energies with optical depth larger than 5% of the 78 keV line. These results indicate that GRO J1008–57 has a magnetic field of 6.7 × 10{sup 12}(1 + z) G, the highest among known accreting pulsars.

  4. Induction of DnaK and GroEL heat shock proteins by fluoroquinolones in Escherichia coli.

    OpenAIRE

    Mizushima, T.; Matsuo, M.; Sekimizu, K

    1997-01-01

    Various fluoroquinolones (norfloxacin, enoxacin, ofloxacin, levofloxacin, and sparfloxacin) induce DnaK and GroEL heat shock proteins in Escherichia coli. The induction is transient, consistent with the kinetics of cellular DNA relaxation. The concentrations of fluoroquinolones required for induction are similar to those required for DNA relaxation and much higher than those required for cell death.

  5. Predominant recognition of species-specific determinants of the GroES homologues from Mycobacterium leprae and M. tuberculosis

    NARCIS (Netherlands)

    Chua-Intra, B.; Ivanyi, J.; Hills, A.; Thole, J.; Moreno, C.; Vordermeier, H.M.

    1998-01-01

    The Mycobacterium leprae and M. tuberculosis 10000 MW heat-shock protein homologues of GroES have previously been identified as major immunogens for human T cells. We used synthetic peptides to characterize the determinants recognized by murine T cells. The findings suggest that, despite 90% sequenc

  6. Simultaneous NuSTAR/Chandra Observations of The Bursting Pulsar GRO J1744-28 During Its Third Reactivation

    DEFF Research Database (Denmark)

    Younes, G.; Kouveliotou, C.; Grefenstette, B. W.

    2015-01-01

    We report on a 10 ks simultaneous Chandra/High Energy Transmission Grating (HETG)-Nuclear Spectroscopic Telescope Array (NuSTAR) observation of the Bursting Pulsar, GRO J1744-28, during its third detected outburst since discovery and after nearly 18 yr of quiescence. The source is detected up to ...

  7. Assessing Reliability and Validity of the "GroPromo" Audit Tool for Evaluation of Grocery Store Marketing and Promotional Environments

    Science.gov (United States)

    Kerr, Jacqueline; Sallis, James F.; Bromby, Erica; Glanz, Karen

    2012-01-01

    Objective: To evaluate reliability and validity of a new tool for assessing the placement and promotional environment in grocery stores. Methods: Trained observers used the "GroPromo" instrument in 40 stores to code the placement of 7 products in 9 locations within a store, along with other promotional characteristics. To test construct validity,…

  8. Discovery of a non-blazar $\\gamma$-ray transient near the Galactic plane GRO J1838-04

    CERN Document Server

    Tavani, M; Mattox, J R; Halpern, J; Thompson, D J; Kanbach, G; Hermsen, W; Zhang, S N; Foster, R S

    1997-01-01

    We report the discovery of a remarkable gamma-ray transient source near the Galactic plane, GRO J1838-04. This source was serendipitously discovered by EGRET in June 1995 with a peak intensity of 4 E(-6) ph/cm/cm/s (for photon energies larger than 100 MeV) and a 5.9 sigma significance. At that time, GRO J1838-04 was the second brightest gamma-ray source in the sky. A subsequent EGRET pointing in late September 1995 detected the source at a flux smaller than its peak value by a factor of 7. We determined that no radio-loud spectrally-flat blazar is within the error box of GRO J1838-04. We discuss the origin of the \\ggg-ray transient source and show that interpretations in terms of AGNs or isolated pulsars are highly problematic. GRO J1838-04 provides strong evidence for the existence of a new class of variable gamma-ray sources.

  9. Effects of the prebiotics GroBiotic-A and inulin on the intestinal microbiota of red drum, Sciaenops ocellatus

    Science.gov (United States)

    Two separate feeding trials examined the effects of dietary supplementation of the prebiotics GroBiotic®-A and inulin on growth performance and gastrointestinal tract microbiota of the red drum Sciaenops ocellatus. In the first feeding trial, fishmeal-based diets without prebiotics or supplemented ...

  10. SIMULTANEOUS FITS IN ISIS ON THE EXAMPLE OF GRO J1008–57

    Directory of Open Access Journals (Sweden)

    M. Kühnel

    2015-04-01

    Full Text Available Parallel computing and steadily increasing computation speed have led to a new tool for analyzing multiple datasets and datatypes: fitting several datasets simultaneously.  With this technique, physically connected parameters of individual data can be treated as a single parameter by implementing this connection directly into the fit. We discuss the terminology, implementation, and possible issues of simultaneous fits based on the Interactive Spectral Interpretation System (ISIS X-ray data analysis tool. While all data modeling tools in X-ray astronomy in principle allow data to be fitted individually from multiple data sets, the syntax used in these tools is not often well suited for this task. Applying simultaneous fits to the transient X-ray binary GRO J1008–57, we find that the spectral shape is only dependent on X-ray flux. We determine time independent parameters e.g., the folding energy Efold, with unprecedented precision.

  11. Simultaneous fits in ISIS on the example of GRO J1008-57

    CERN Document Server

    Kühnel, Matthias; Kreykenbohm, Ingo; Schwarm, Fritz-Walter; Grossberger, Christoph; Dauser, Thomas; Pottschmidt, Katja; Ferrigno, Carlo; Rothschild, Richard E; Klochkov, Dmitry; Staubert, Rüdiger; Wilms, Jörn

    2016-01-01

    Parallel computing and steadily increasing computation speed have led to a new tool for analyzing multiple datasets and datatypes: fitting several datasets simultaneously. With this technique, physically connected parameters of individual data can be treated as a single parameter by implementing this connection into the fit directly. We discuss the terminology, implementation, and possible issues of simultaneous fits based on the X-ray data analysis tool Interactive Spectral Interpretation System (ISIS). While all data modeling tools in X-ray astronomy allow in principle fitting data from multiple data sets individually, the syntax used in these tools is not often well suited for this task. Applying simultaneous fits to the transient X-ray binary GRO J1008-57, we find that the spectral shape is only dependent on X-ray flux. We determine time independent parameters such as, e.g., the folding energy E_fold, with unprecedented precision.

  12. Abstracts of the 28. annual meeting of the Austrian Radiation Oncology, Radiation Biology and Medical Radiation Physics Society (OeGRO 2011); Abstracts der 28. Jahrestagung der Oesterreichischen Gesellschaft fuer Radioonkologie, Radiobiologie und Medizinische Radiophysik (OeGRO 2011)

    Energy Technology Data Exchange (ETDEWEB)

    Anon.

    2012-06-15

    The second part of the volume includes the abstracts of the 28th annual meeting of the Austrian Radiation Oncology, Radiation Biology and Medical Radiation Physics Society (OeGRO 2011), covering the following topics: extracranial stereotactic radiotherapy; brachytherapy, hyperthermia; radiotherapy side effects; psycho-oncology in radiotherapy; head-neck carcinomas; radiation source implants for carcinoma irradiation; MRI-supported adaptive radiotherapy; CT-guided radiotherapy; mammary carcinomas; prostate carcinomas; magnetic nanoparticles for future medical applications.

  13. The lesional skin of linear IgA bullous dermatosis expresses growth-regulated peptide (GRO)-alpha.

    Science.gov (United States)

    Tsunemi, Yuichiro; Ihn, Hironobu; Saeki, Hidehisa; Tamaki, Kunihiko

    2004-07-01

    The patient was a 62-year-old man with erythema with tense vesiculobullae and erosions on the bilateral elbows, right knee, and one buttock. A skin biopsy specimen revealed subepidermal blister formation with a predominant infiltration of neutrophils and papillary neutrophilic microabscesses. Direct immunofluorescence study showed linear deposition of IgA and weak deposition of IgG at the basement membrane zone of the lesional skin, and indirect immunofluorescence study showed linear deposition of IgA at the epidermal side of the 1M NaCl-separated normal skin. He was diagnosed with linear IgA bullous dermatosis. Immunohistochemical study revealed that the lesional and perilesional keratinocytes expressed growth-regulated peptide (GRO) -alpha, a potent chemoattractant for neutrophils. This suggests that GRO-alpha plays a role in the infiltration of neutrophils into the lesional skin and in bulla formation in linear IgA bullous dermatosis.

  14. GRO J1655-40: Early Stages of the 2005 Outburst

    CERN Document Server

    Shaposhnikov, N; Shrader, C R; Rupen, M; Beckmann, V; Markwardt, C B; Smith, D A

    2006-01-01

    The black-hole X-ray binary transient GRO J1655-40 underwent an outburst beginning in early 2005. We present the results of our multi-wavelength observational campaign to study the early outburst spectral and temporal evolution, which combines data from X-ray (RXTE, INTEGRAL), radio (VLA) and optical (ROTSE, SMARTS) instruments. During the reported period the source left quiescence and went through four major accreting black hole states: low-hard, hard intermediate, soft intermediate and high-soft. We investigated dipping behavior in the RXTE band and compare our results to the 1996-1997 case, when the source was predominantly in the high-soft state, finding significant differences. We consider the evolution of the low frequency quasi-periodic oscillations and find that the frequency strongly correlates with the spectral characteristics, before shutting off prior to the transition to the high-soft state. We model the broad-band high-energy spectrum in the context of empirical models, as well as more physicall...

  15. Allosteric transitions of supramolecular systems explored by network models: application to chaperonin GroEL.

    Directory of Open Access Journals (Sweden)

    Zheng Yang

    2009-04-01

    Full Text Available Identification of pathways involved in the structural transitions of biomolecular systems is often complicated by the transient nature of the conformations visited across energy barriers and the multiplicity of paths accessible in the multidimensional energy landscape. This task becomes even more challenging in exploring molecular systems on the order of megadaltons. Coarse-grained models that lend themselves to analytical solutions appear to be the only possible means of approaching such cases. Motivated by the utility of elastic network models for describing the collective dynamics of biomolecular systems and by the growing theoretical and experimental evidence in support of the intrinsic accessibility of functional substates, we introduce a new method, adaptive anisotropic network model (aANM, for exploring functional transitions. Application to bacterial chaperonin GroEL and comparisons with experimental data, results from action minimization algorithm, and previous simulations support the utility of aANM as a computationally efficient, yet physically plausible, tool for unraveling potential transition pathways sampled by large complexes/assemblies. An important outcome is the assessment of the critical inter-residue interactions formed/broken near the transition state(s, most of which involve conserved residues.

  16. The quiescent light curve and orbital period of GRO$\\sim$J0422+32

    CERN Document Server

    Chevalier, C; Chevalier, Claude; Ilovaisky, Sergio A

    1996-01-01

    CCD photometry of the black hole candidate GRO~J0422+32 in quiescence, obtained at Haute-Provence from 1994 November to 1995 February, reveals a double-wave modulation at a period close to the value we found during outbursts and also close to one of the possible periods derived by Filippenko, Matheson and Ho (1995) from spectroscopic observations with the W. M. Keck 10-m telescope. A period of 0.212140 \\pm 0.000003 d (5.09136 \\pm 0.00007 h) fits all our photometric data from 1993 January to 1995 February and yields a minimum in our light curves at the inferior conjunction of the M star, as determined from the radial velocity data of Filippenko et al. (1995). The quiescent R_{c} band light curve exhibits a changing asymmetry of shape and a variable amplitude. On two consecutive nights the source was found constant to within \\pm 0.05 mag, suggesting an upper limit on the ellipsoidal effect in this band.

  17. Vanadyl ions binding to GroEL (HSP60) and inducing its depolymerization

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Several vanadium compounds have been known for the hypoglycemic and anticancer effects. However, the mechanisms of the pharmacological and toxicological effects were not clear. In this work, we investigated the potential targets of vanadium in mitochondria. Vanadyl ions were found to bind to mitochondria from rat liver with a stoichiometry of 244±58 nmol/mg protein and an apparent dissocia- tion constant (Kd) of (2.0±0.8)×10-16 mol/L. Using size exclusion chromatography, a vanadium-binding protein was isolated and identified to be the 60-kDa heat shock protein (HSP60) by mass spectrometry analysis and immunoassays. Additionally, binding of vanadyl ions was found to result in depolymerization of homo-oligomeric HSP60 (GroEL). HSP60 is an indispensable molecular chaperone and involved in many kinds of pathogenesis of inflammatory and autoimmune diseases, e.g. type 1 diabetes. Our results suggested that HSP60 could be a novel important target involved in the biological and/or toxicological effects of vanadium compounds.

  18. Spectral and Timing evolution of GRO J1655-40 during its outburst of 2005

    CERN Document Server

    Debnath, D; Nandi, A; Mandal, S

    2009-01-01

    In a recent outburst which lasted for 260 days, the black hole candidate GRO J1655-40 exhibited a behaviour similar to its last outburst observed almost eight years ago. We analyze a total of 150 observational spells in 122 days of data spreaded over the entire outburst phase of Feb. 2005 to Oct. 2005. From our study, a comprehensive understanding of the detailed behaviour of this black hole candidate has emerged. Based on the degree of importance of the black body and the power-law components we divide the entire episode in four spectral states, namely, hard, soft, very soft and intermediate. Quasi-Periodic oscillations (QPOs) were found in two out of these four states, namely, in the hard and the intermediate states. In the hard state, at the rising phase of the outburst, QPO frequency ranged from 0.034 - 17.78Hz and the spectra was fitted by a disk black body, power-law and iron emission line at 6.2 - 6.5 keV. In the intermediate state, QPOs vary from 13.17Hz to 19.04Hz and the QPO frequency modulation in ...

  19. High-Resolution X-Ray Spectroscopy of the Bursting Pulsar GRO J1744-28

    CERN Document Server

    Degenaar, N; Harrison, F A; Kennea, J A; Kouveliotou, C; Younes, G

    2014-01-01

    The bursting pulsar GRO J1744-28 is a Galactic low-mass X-ray binary that distinguishes itself by displaying type-II X-ray bursts: brief, bright flashes of X-ray emission that likely arise from spasmodic accretion. Combined with its coherent 2.1 Hz X-ray pulsations and relatively high estimated magnetic field, it is a particularly interesting source to study the physics of accretion flows around neutron stars. Here we report on Chandra/HETG observations obtained near the peak of its bright 2014 accretion outburst. Spectral analysis suggests the presence of a broad iron emission line centered at E_l ~ 6.7 keV. Fits with a disk reflection model yield an inclination angle of i ~ 52 degrees and an inner disk radius of R_in ~ 85 GM/c^2, which is much further out than typically found for neutron star low-mass X-ray binaries. Assuming that the disk is truncated at the magnetospheric radius of the neutron star, we estimate a magnetic field strength of B ~ (2-6)E10 G. Furthermore, we identify an absorption feature nea...

  20. A Super-Eddington Compton-Thick Wind in GRO J1655-40?

    CERN Document Server

    Neilsen, J; Homan, J; Buxton, M

    2016-01-01

    During its 2005 outburst, GRO J1655-40 was observed at high spectral resolution with the Chandra HETGS, revealing a spectrum rich with blueshifted absorption lines indicative of an accretion disk wind -- apparently too hot, too dense, and too close to the black hole to be driven by radiation pressure or thermal pressure (Miller et al.). But this exotic wind represents just one piece of the puzzle in this outburst, as its presence coincides with an extremely soft and curved X-ray continuum spectrum, remarkable X-ray variability (Uttley & Klein-Wolt), and a bright, unexpected optical/infrared blackbody component that varies on the orbital period. Focusing on the X-ray continuum and the optical/infrared/UV spectral energy distribution, we argue that the unusual features of this "hypersoft state" are natural consequences of a super-Eddington Compton-thick wind from the disk: the optical/infrared blackbody represents the cool photosphere of a dense, extended outflow, while the X-ray emission is explained as Co...

  1. 表达大肠杆菌分子伴侣GroEL、 GroES和GrpE载体的构建及其应用%Construction of the Vector Expressing GroEL 、 GroES and GrpE from Escherchia coli and Its Application

    Institute of Scientific and Technical Information of China (English)

    许鹏; 王蕾蕾; 程备久; 范军

    2012-01-01

    Coexpression system in Escherichia coli often requires the different plasmids with different antibiotic resistance genes and replicons. Using sticky-end PCR cloning technology, two independent,PCR reactions were performed for amplifying the artificial operon from pR-GESP plasmid, which contains the artificial polycistron of GroEL, GroES and GrpE genes from E. coli encoding three molecular chaperones. The cohesive end of the PCR products allowed to be inserted into pACYCDuet-1 plasmid treated with Ncol and Xhol. The constructed plasmid named pA-GESP contained the artificial operon, pl5A replicon and chloramphenicol resistance gene, compatible with pET-28b plasmid including ColEl replicon and kanamycin resistance gene. The SDS-PAGE analysis showed that three chaperones were expressed in soluble form from the induced E. coli cells carrying pA-GESP plasmid, similar to the cells harboring pR-GESP plasmid. Co-expression of three molecular chaperones partly improved the soluble expression of recombinant maize serine racemase, but did not have an affect on the expression of maize ferrodioxin reductase. Co-expression of three molecular chaperones, yeast 5-aminolevulinate synthase and Bacillus subtilis uroporphyrinogen methyltransferase from three plasmids containing the different antibiotics resistance gene increased the accumulation of intracellular red fluorescent products catalyzed by two enzymes successively. The production was estimated about 562. 13 ±3. 17/OD600 , compared with the production of 457. 66 ±4. 98/OD600 without function of the recombinant chaperones. The results suggested three chaperones partly improved the soluble expression of some proteins and catalytic activity in E. coli.%大肠杆菌(Escherichia coli)共表达系统常要求质粒具有不同抗生素抗性以及不同的复制子.利用粘性末端PCR技术,以含有大肠杆菌分子伴侣基因GroEL、GroES和GrpE的pR-GESP质粒为模板,设计两对引物,通

  2. Multiplex PCR for the detection and differentiation of Vibrio parahaemolyticus strains using the groEL, tdh and trh genes.

    Science.gov (United States)

    Hossain, Muhammad Tofazzal; Kim, Young-Ok; Kong, In-Soo

    2013-01-01

    Vibrio parahaemolyticus is a significant cause of human gastrointestinal disorders worldwide, transmitted primarily by ingestion of raw or undercooked contaminated seafood. In this study, a multiplex PCR assay for the detection and differentiation of V. parahaemolyticus strains was developed using primer sets for a species-specific marker, groEL, and two virulence markers, tdh and trh. Multiplex PCR conditions were standardised, and extracted genomic DNA of 70 V. parahaemolyticus strains was used for identification. The sensitivity and efficacy of this method were validated using artificially inoculated shellfish and seawater. The expected sizes of amplicons were 510 bp, 382 bp, and 171 bp for groEL, tdh and trh, respectively. PCR products were sufficiently different in size, and the detection limits of the multiplex PCR for groEL, tdh and trh were each 200 pg DNA. Specific detection and differentiation of virulent from non-virulent strains in shellfish homogenates and seawater was also possible after artificial inoculation with various V. parahaemolyticus strains. This newly developed multiplex PCR is a rapid assay for detection and differentiation of pathogenic V. parahaemolyticus strains, and could be used to prevent disease outbreaks and protect public health by helping the seafood industry maintain a safe shellfish supply.

  3. Making a friend from a foe: expressing a GroEL gene from the whitefly Bemisia tabaci in the phloem of tomato plants confers resistance to tomato yellow leaf curl virus.

    Science.gov (United States)

    Akad, F; Eybishtz, A; Edelbaum, D; Gorovits, R; Dar-Issa, O; Iraki, N; Czosnek, H

    2007-01-01

    Some (perhaps all) plant viruses transmitted in a circulative manner by their insect vectors avoid destruction in the haemolymph by interacting with GroEL homologues, ensuring transmission. We have previously shown that the phloem-limited begomovirus tomato yellow leaf curl virus (TYLCV) interacts in vivo and in vitro with GroEL produced by the whitefly vector Bemisia tabaci. In this study, we have exploited this phenomenon to generate transgenic tomato plants expressing the whitefly GroEL in their phloem. We postulated that following inoculation, TYLCV particles will be trapped by GroEL in the plant phloem, thereby inhibiting virus replication and movement, thereby rendering the plants resistant. A whitefly GroEL gene was cloned in an Agrobacterium vector under the control of an Arabidopsis phloem-specific promoter, which was used to transform two tomato genotypes. During three consecutive generations, plants expressing GroEL exhibited mild or no disease symptoms upon whitefly-mediated inoculation of TYLCV. In vitro assays indicated that the sap of resistant plants contained GroEL-TYLCV complexes. Infected resistant plants served as virus source for whitefly-mediated transmission as effectively as infected non-transgenic tomato. Non-transgenic susceptible tomato plants grafted on resistant GroEL-transgenic scions remained susceptible, although GroEL translocated into the grafted plant and GroEL-TYLCV complexes were detected in the grafted tissues.

  4. CcpA affects expression of the groESL and dnaK operons in Lactobacillus plantarum

    Directory of Open Access Journals (Sweden)

    Marasco Rosangela

    2006-11-01

    Full Text Available Abstract Background Lactic acid bacteria (LAB are widely used in food industry and their growth performance is important for the quality of the fermented product. During industrial processes changes in temperature may represent an environmental stress to be overcome by starters and non-starters LAB. Studies on adaptation to heat shock have shown the involvement of the chaperon system-proteins in various Gram-positive bacteria. The corresponding operons, namely the dnaK and groESL operons, are controlled by a negative mechanism involving the HrcA repressor protein binding to the cis acting element CIRCE. Results We studied adaptation to heat shock in the lactic acid bacterium Lactobacillus plantarum. The LM3-2 strain, carrying a null mutation in the ccpA gene, encoding the catabolite control protein A (CcpA, showed a lower percent of survival to high temperature with respect to the LM3 wild type strain. Among proteins differentially expressed in the two strains, the GroES chaperon was more abundant in the wild type strain compared to the mutant strain under standard growth conditions. Transcriptional studies showed that class I heat shock operons were differentially expressed upon heat shock in both strains. Indeed, the dnaK and groESL operons were induced about two times more in the LM3 strain compared to the LM3-2 strain. Analysis of the regulatory region of the two operons showed the presence of cre sequences, putative binding sites for the CcpA protein. Conclusion The L. plantarum dnaK and groESL operons are characterized by the presence of the cis acting sequence CIRCE in the promoter region, suggesting a negative regulation by the HrcA/CIRCE system, which is a common type of control among the class I heat shock operons of Gram-positive bacteria. We found an additional system of regulation, based on a positive control exerted by the CcpA protein, which would interact with cre sequences present in the regulatory region of the dnaK and gro

  5. Gene cloning and expression analysis of Gro/KC by primary hepatocytes

    Directory of Open Access Journals (Sweden)

    GH. Hassanshahi

    2007-01-01

    Full Text Available Abstract Background and purpose: It is now well established that several environmental stresses lead to activation of p38 MAP kinase and JNK in various cell systems which is followed by chemokine producion. We investigated the expression of both CXC chemokines SDF-1a (ELR- and Gro/KC (ELR+ in rat H4 hepatoma cells in response to heat shock, hyper-osmolarity and oxidative stresses. The pattern of expression of these chemokines by hepatoma cells in response to stress conditions was also studied.Materials and Methods: Hepatoma cells were maintained in MEM medium. Cells were subjected to different stresses [(H2O2 0.15% (w/v, manitol and NaCl (160 mM and heat shock [(42 °C for 20 minutes]. At the indicated time points, cells were harvested and RNA was extracted, purified and expression of the chemokines were analysed by RT-PCR. cDNA was separated by gel electrophoresis on a 1% (w/v agarose gel and visualised on a UV transilluminator.Results: Results obtained in this report showed that there was detectable but low expression of chemokines in H4 hepatoma cells. Heat shock failed to induce expression of chemokines in H4 rat hepatoma cells. Hyper-osmolarity also has not stimulated Chemokines expression. In this study we have also shown that oxidative stress did not induce expression of chemokines. Overall, although detection is possible but regulatory responses were not observed in H4 hepatoma cells.Conclusion: Several known injurious conditions cause recruitment of macrophages, neutrophils and other immune cells to the liver. Immune cells are recruited to the hepatic vasculature following local liver injury and consequent chemokine production. Our results demonstrated that failure in production of these chemokines by Hepatoma cells may be a way to escape from immune surveillance.

  6. HIGH-RESOLUTION X-RAY SPECTROSCOPY OF THE BURSTING PULSAR GRO J1744-28

    Energy Technology Data Exchange (ETDEWEB)

    Degenaar, N.; Miller, J. M. [Department of Astronomy, University of Michigan, 1085 South University Avenue, Ann Arbor, MI 48109 (United States); Harrison, F. A. [Cahill Center for Astronomy and Astrophysics, California Institute of Technology, Pasadena, CA 91125 (United States); Kennea, J. A. [Department of Astronomy and Astrophysics, 525 Davey Lab, Pennsylvania State University, University Park, PA 16802 (United States); Kouveliotou, C. [Space Science Office, ZP12, NASA Marshall Space Flight Center, Huntsville, AL 35812 (United States); Younes, G., E-mail: degenaar@umich.edu [Universities Space Research Association, 6767 Old Madison Pike, Suite 450, Huntsville, AL 35806 (United States)

    2014-11-20

    The bursting pulsar GRO J1744-28 is a Galactic low-mass X-ray binary that distinguishes itself by displaying type-II X-ray bursts: brief, bright flashes of X-ray emission that likely arise from spasmodic accretion. Combined with its coherent 2.1 Hz X-ray pulsations and relatively high estimated magnetic field, it is a particularly interesting source to study the physics of accretion flows around neutron stars. Here we report on Chandra/High Energy Transmission Grating observations obtained near the peak of its bright 2014 accretion outburst. Spectral analysis suggests the presence of a broad iron emission line centered at E {sub l} ≅ 6.7 keV. Fits with a disk reflection model yield an inclination angle of i ≅ 52° and an inner disk radius of R {sub in} ≅ 85 GM/c {sup 2}, which is much further out than typically found for neutron star low-mass X-ray binaries. Assuming that the disk is truncated at the magnetospheric radius of the neutron star, we estimate a magnetic field strength of B ≅ (2-6) × 10{sup 10} G. Furthermore, we identify an absorption feature near ≅ 6.85 keV that could correspond to blue-shifted Fe XXV and point to a fast disk wind with an outflow velocity of v {sub out} ≅ (7.5-8.2) × 10{sup 3} km s{sup –1} (≅ 0.025c-0.027c). If the covering fraction and filling factor are large, this wind could be energetically important and perhaps account for the fact that the companion star lost significant mass while the magnetic field of the neutron star remained strong.

  7. Activation of phagocytic cells by Staphylococcus epidermidis biofilms: effects of extracellular matrix proteins and the bacterial stress protein GroEL on netosis and MRP-14 release.

    Science.gov (United States)

    Dapunt, Ulrike; Gaida, Matthias M; Meyle, Eva; Prior, Birgit; Hänsch, Gertrud M

    2016-07-01

    The recognition and phagocytosis of free-swimming (planktonic) bacteria by polymorphonuclear neutrophils have been investigated in depth. However, less is known about the neutrophil response towards bacterial biofilms. Our previous work demonstrated that neutrophils recognize activating entities within the extracellular polymeric substance (EPS) of biofilms (the bacterial heat shock protein GroEL) and that this process does not require opsonization. Aim of this study was to evaluate the release of DNA by neutrophils in response to biofilms, as well as the release of the inflammatory cytokine MRP-14. Neutrophils were stimulated with Staphylococcus epidermidis biofilms, planktonic bacteria, extracted EPS and GroEL. Release of DNA and of MRP-14 was evaluated. Furthermore, tissue samples from patients suffering from biofilm infections were collected and evaluated by histology. MRP-14 concentration in blood samples was measured. We were able to show that biofilms, the EPS and GroEL induce DNA release. MRP-14 was only released after stimulation with EPS, not GroEL. Histology of tissue samples revealed MRP-14 positive cells in association with neutrophil infiltration and MRP-14 concentration was elevated in blood samples of patients suffering from biofilm infections. Our data demonstrate that neutrophil-activating entities are present in the EPS and that GroEL induces DNA release by neutrophils.

  8. Simultaneous NuSTAR/Chandra Observations of The Bursting Pulsar GRO J1744-28 During Its Third Reactivation

    DEFF Research Database (Denmark)

    Younes, G.; Kouveliotou, C.; Grefenstette, B. W.;

    2015-01-01

    keV with an Eddington persistent flux level. Seven bursts, followed by dips, are seen with Chandra, three of which are also detected with NuSTAR. Timing analysis reveals a slight increase in the persistent emission pulsed fraction with energy (from 10% to 15%) up to 10 keV, above which it remains......We report on a 10 ks simultaneous Chandra/High Energy Transmission Grating (HETG)-Nuclear Spectroscopic Telescope Array (NuSTAR) observation of the Bursting Pulsar, GRO J1744-28, during its third detected outburst since discovery and after nearly 18 yr of quiescence. The source is detected up to 60...

  9. Increased function of the TRPV1 channel in small sensory neurons after local inflammation or in vitro exposure to the pro-inflammatory cytokine GRO/KC

    Institute of Scientific and Technical Information of China (English)

    Fei Dong; Yi-Ru Du; Wenrui Xie; Judith A.Strong; Xi-Jing He; Jun-Ming Zhang

    2012-01-01

    Objective Inflammation at the level of the sensory dorsal root ganglia (DRGs) leads to robust mechanical pain behavior and the local inflammation has direct excitatory effects on sensory neurons including small,primarily nociceptive,neurons.These neurons express the transient receptor potential vanilloid-1 (TRPV 1) channel,which integrates multiple signals of pain and inflammation.The aim of this study was to characterize the regulation of the TRPV1 channel by local DRG inflammation and by growth-related oncogene (GRO/KC,systemic name:CXCL1),a cytokine known to be upregulated in inflamed DRGs.Methods Activation of the TRPV1 receptor with capsaicin was studied with patch clamp methods in acutely isolated small-diameter rat sensory neurons in primary culture.In vivo,behavioral effects of TRPV1 and GRO/KC were examined by paw injections.Results Neurons isolated from lumbar DRGs 3 days after local inflammation showed enhanced TRPV1 function:tachyphylaxis (the decline in response to repeated applications of capsaicin) was significantly reduced.A similar effect on tachyphylaxis was observed in neurons pre-treated for 4 h in vitro with GRO/KC.This effect was blocked by H-89,a protein kinase A inhibitor.Consistent with the in vitro results,in vivo behavioral responses to paw injection of capsaicin were enhanced and prolonged by pre-injecting the paw with GRO/KC 4 h before the capsaicin injection.GRO/KC paw injections alone did not elicit pain behaviors.Conclusion Function of the TRPV 1 channel is enhanced by DRG inflammation and these effects are preserved in vitro during short-term culture.The effects (decreased tachyphylaxis) are mimicked by incubation with GRO/KC,which has previously been found to be strongly upregulated in this and other pain models.

  10. INTEGRAL detection of the multi-peaked emission from the Be/X-ray binary pulsar GRO J1008-57

    DEFF Research Database (Denmark)

    Fiocchi, M.; Sguera, A.; Chenevez, Jérôme

    2014-01-01

    Recent observations from the on-going INTEGRAL Galactic Plane Scanning programme (PI: A. Bazzano) have detected increasing X-ray flux from the Be/X-ray binary pulsar GRO J1008-57, confirming the re-brightening detected by MAXI/GSC (ATel #6819). The source was in the field of view of the IBIS...

  11. Electron microscopy and image analysis of the GroEL-like protein and its complexes with glutamine synthetase from pea leaves

    NARCIS (Netherlands)

    Tsuprun, Vladimir L.; Boekema, Egbert J.; Pushkin, Alexander V.; Tagunova, Irina V.

    1992-01-01

    The molecular structure of groEL-like protein from pea leaves has been studied by electron microscopy and image analysis of negatively stained particles. Over 1500 molecular projections were selected and classified by multivariate statistical analysis. It was shown that the molecule consists of 14 s

  12. Growth performance and resistance to Streptococcus iniae of juvenile Nile tilapia (Oreochromis niloticus) fed diets supplemented with GroBiotic - A and Brewtech Dried Brewers Yeast

    Science.gov (United States)

    This study was conducted to evaluate the effect of dietary levels of Brewtech® dried brewers yeast (BY) and GroBiotic®-A (GB) on growth performance, proximate body composition, immune response and resistance of juvenile Nile tilapia to Streptococcus iniae challenge. A practical basal (control) diet ...

  13. Detection of the Host Immune Response to Burkholderia mallei Heat-Shock Proteins GroEL and DnaK in a Glanders Patient and Infected Mice

    Science.gov (United States)

    2007-01-01

    infected animal are the most common methods of acquiring glanders because the organism can be trans- mitted through droplets or saliva . In horses...host cell. A GroEL-like protein in Actinobacillus actinomycetemcomitans, a patho- gen associated with periodontal disease, was found in extracellular

  14. Detection of the host immune response to Burkholderia mallei heat-shock proteins GroEL and DnaK in a glanders patient and infected mice.

    Science.gov (United States)

    Amemiya, Kei; Meyers, Jennifer L; Deshazer, David; Riggins, Renaldo N; Halasohoris, Stephanie; England, Marilyn; Ribot, Wilson; Norris, Sarah L; Waag, David M

    2007-10-01

    We examined, by enzyme-linked immunosorbent assay and Western blot analysis, the host immune response to 2 heat-shock proteins (hsps) in a patient and mice previously infected with Burkholderia mallei. The patient was the first reported human glanders case in 50 years in the United States. The expression of the groEL and dnaK operons appeared to be dependent upon a sigma(32) RNA polymerase as suggested by conserved heat-shock promoter sequences, and the groESL operon may be negatively regulated by a controlling invert repeat of chaperone expression (CIRCE) site. In the antisera, the GroEL protein was found to be more immunoreactive than the DnaK protein in both a human patient and mice previously infected with B. mallei. Examination of the supernatant of a growing culture of B. mallei showed that more GroEL protein than DnaK protein was released from the cell. This may occur similarly within an infected host causing an elevated host immune response to the B. mallei hsps.

  15. Nucleolin inhibitor GroA triggers reduction in epidermal growth factor receptor activation: Pharmacological implication for glial scarring after spinal cord injury.

    Science.gov (United States)

    Goldshmit, Yona; Schokoroy Trangle, Sari; Afergan, Fabian; Iram, Tal; Pinkas-Kramarski, Ronit

    2016-09-01

    Glial scarring, formed by reactive astrocytes, is one of the major impediments for regeneration after spinal cord injury (SCI). Reactive astrocytes become hypertrophic, proliferate and secrete chondroitin sulphate proteoglycans into the extracellular matrix (ECM). Many studies have demonstrated that epidermal growth factor receptors (EGFR) can mediate astrocyte reactivity after neurotrauma. Previously we showed that there is crosstalk between nucleolin and EGFR that leads to increased EGFR activation followed by increased cell proliferation. Treatment with the nucleolin inhibitor GroA (AS1411) prevented these effects in vitro and in vivo. In this study, we hypothesized that similar interactions may mediate astrogliosis after SCI. Our results demonstrate that nucleolin and EGFR interaction may play a pivotal role in mediating astrocyte proliferation and reactivity after SCI. Moreover, we demonstrate that treatment with GroA reduces EGFR activation, astrocyte proliferation and chondroitin sulphate proteoglycans secretion, therefore promoting axonal regeneration and sprouting into the lesion site. Our results identify, for the first time, a role for the interaction between nucleolin and EGFR in astrocytes after SCI, indicating that nucleolin inhibitor GroA may be used as a novel treatment after neurotrauma. A major barrier for axonal regeneration after spinal cord injury is glial scar created by reactive and proliferating astrocytes. EGFR mediate astrocyte reactivity. We showed that inhibition of nucleolin by GroA, reduces EGFR activation, which results in attenuation of astrocyte reactivity and proliferation in vivo and in vitro. EGFR, epidermal growth factor receptor.

  16. Bone marrow mast cell density correlates with serum levels of VEGF and CXC chemokines ENA-78 and GRO-α in multiple myeloma.

    Science.gov (United States)

    Pappa, C A; Tsirakis, G; Devetzoglou, M; Zafeiri, M; Vyzoukaki, R; Androvitsanea, A; Xekalou, A; Sfiridaki, K; Alexandrakis, M G

    2014-06-01

    Angiogenesis is a crucial process in growth and progression of multiple myeloma (MM). Mast cells (MCs) play an important role in MM angiogenesis. Various angiogenic mediators secreted by MCs regulate endothelial cell proliferation and function. Among them, ELR(+) CXC chemokines, such as growth-related oncogen-alpha (GRO-α) and epithelial neutrophil activating protein-78 (ENA-78), have been described as potential mediators in regulation of angiogenesis. The purpose of the study was to quantify MCs in bone marrow (BM) biopsies of MM patients, expressed as MC density (MCD), and correlate it with serum concentrations of vascular endothelial factor (VEGF), GRO-α, ENA-78. Fifty-four newly diagnosed MM patients and 22 healthy controls were studied. Tryptase was used for the immunohistochemical stain of MCs. VEGF, GRO-α, and ENA-78 were measured in sera by ELISA. MCD and serum levels of GRO-α, ENA-78, and VEGF were significantly higher in MM patients compared to controls (pENA-78. These findings support that MCs participate in the pathophysiology of MM and is implicated in the angiogenic process and disease progression.

  17. The htpAB operon of Legionella pneumophila cannot be deleted in the presence of the groE chaperonin operon of Escherichia coli.

    Science.gov (United States)

    Nasrallah, Gheyath K; Gagnon, Elizabeth; Orton, Dennis J; Garduño, Rafael A

    2011-11-01

    HtpB, the chaperonin of the intracellular bacterial pathogen Legionella pneumophila , displays several virulence-related functions in vitro. To confirm HtpB's role in vivo, host infections with an htpB deletion mutant would be required. However, we previously reported that the htpAB operon (encoding co-chaperonin and chaperonin) is essential. We attempted here to delete htpAB in a L. pneumophila strain carrying the groE operon (encoding the Escherichia coli co-chaperonin and chaperonin). The groE operon was inserted into the chromosome of L. pneumophila Lp02, and then allelic replacement of htpAB with a gentamicin resistance cassette was attempted. Although numerous potential postallelic replacement transformants showed a correct selection phenotype, we still detected htpAB by PCR and full-size HtpB by immunoblot. Southern blot and PCR analysis indicated that the gentamicin resistance cassette had apparently integrated in a duplicated htpAB region. However, we showed by Southern blot that strain Lp02, and the Lp02 derivative carrying the groE operon, have only one copy of htpAB. These results confirmed that the htpAB operon cannot be deleted, not even in the presence of the groE operon, and suggested that attempts to delete htpAB under strong phenotypic selection result in aberrant genetic recombinations that could involve duplication of the htpAB locus.

  18. Von Nobel bis Viagra: Die Geschichte eines kleinen Moleküls mit großer Wirkung: Stickstoffmonoxyd (NO

    Directory of Open Access Journals (Sweden)

    Philipp T

    2010-01-01

    Full Text Available Es wird kurzgefasst die Geschichte des Nitroglyzerins und von Alfred Nobel dargestellt. Nitroglyzerin war als explosiver Bestandteil von Dynamit der gewaltigste Sprengstoff seiner Zeit und wurde von Alfred Nobel weiterentwickelt und kommerzialisiert, der später den Nobelpreis stiftete. Gleichermaßen ist Nitroglyzerin in öliger Dispersion und in geringsten Mengen seit 100 Jahren als „Koronardilatator“ eingeführt und weiterhin nahezu unersetzlich. Der Mechanismus, mit dem Nitroglyzerin Gefäße erweitert, wurde erst vor 25 Jahren von Furchgott, Ignarro und Murad vollständig aufgeklärt, indem sie die große Bedeutung des Endothels und des gefäßerweiternden Stickstoffmonoxyds beschrieben. Die 3 Forscher erhielten 1998 den Nobelpreis für Medizin zu gleichen Teilen "für die Entdeckung des NOs als Signalmolekül für das kardiovaskuläre System".

  19. Chaperonin GroEL a Brucella immunodominant antigen identified using Nanobody and MALDI-TOF-MS technologies.

    Science.gov (United States)

    Abbady, A Q; Al-Daoude, A; Al-Mariri, A; Zarkawi, M; Muyldermans, S

    2012-05-15

    The deployment of today's antibodies that are able to distinguish Brucella from the closely similar pathogens, such as Yersinia, is still considered a great challenge since both pathogens share identical LPS (lipopolysaccharide) O-ring epitopes. In addition, because of the great impact of Brucella on health and economy in many countries including Syria, much effort is going to the development of next generation vaccines, mainly on the identification of new immunogenic proteins of this pathogen. In this context, Brucella-specific nanobodies (Nbs), camel genetic engineered heavy-chain antibody fragments, could be of great value. Previously, a large Nb library was constructed from a camel immunized with heat-killed Brucella. Phage display panning of this 'immune' library with Brucella total lysate resulted in a remarkable fast enrichment for a Nb referred to as NbBruc02. In the present work, we investigated the main characteristics of this Nb that can efficiently distinguish under well-defined conditions the Brucella from other bacteria including Yersinia. NbBruc02 showed a strong and specific interaction with its antigen within the crude lysate as tested by a surface plasmon resonance (SPR) biosensor and it was also able to pull down its cognate antigen from such lysate by immuno-capturing. Using matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS), NbBruc02 specific antigen was identified as chaperonin GroEL, also known as heat shock protein of 60 kDa (HSP-60), which represents a Brucella immunodominant antigen responsible of maintaining proteins folding during stress conditions. Interestingly, the antigen recognition by NbBruc02 was found to be affected by the state of GroEL folding. Thus, the Nb technology applied in the field of infectious diseases, e.g. brucellosis, yields two outcomes: (1) it generates specific binders that can be used for diagnosis, and perhaps treatment, and (2) it identifies the immunogenic candidate

  20. Enhanced translocation and growth of Rhodococcus erythropolis PR4 in the alkane phase of aqueous-alkane two phase cultures were mediated by GroEL2 overexpression.

    Science.gov (United States)

    Takihara, Hayato; Ogihara, Jun; Yoshida, Takao; Okuda, Shujiro; Nakajima, Mutsuyasu; Iwabuchi, Noriyuki; Sunairi, Michio

    2014-01-01

    We previously reported that R. erythropolis PR4 translocated from the aqueous to the alkane phase, and then grew in two phase cultures to which long-chain alkanes had been added. This was considered to be beneficial for bioremediation. In the present study, we investigated the proteins involved in the translocation of R. erythropolis PR4. The results of our proteogenomic analysis suggested that GroEL2 was upregulated more in cells that translocated inside of the pristane (C19) phase than in those located at the aqueous-alkane interface attached to the n-dodecane (C12) surface. PR4 (pK4-EL2-1) and PR4 (pK4-ΔEL2-1) strains were constructed to confirm the effects of the upregulation of GroEL2 in translocated cells. The expression of GroEL2 in PR4 (pK4-EL2-1) was 15.5-fold higher than that in PR4 (pK4-ΔEL2-1) in two phase cultures containing C12. The growth and cell surface lipophilicity of PR4 were enhanced by the introduction of pK4-EL2-1. These results suggested that the plasmid overexpression of groEL2 in PR4 (pK4-EL2-1) led to changes in cell localization, enhanced growth, and increased cell surface lipophilicity. Thus, we concluded that the overexpression of GroEL2 may play an important role in increasing the organic solvent tolerance of R. erythropolis PR4 in aqueous-alkane two phase cultures.

  1. Binding of Haemophilus ducreyi to carbohydrate receptors is mediated by the 58.5-kDa GroEL heat shock protein.

    Science.gov (United States)

    Pantzar, Martina; Teneberg, Susann; Lagergård, Teresa

    2006-08-01

    The bacterium Haemophilus ducreyi causes the sexually transmitted disease chancroid, which is characterized by the appearance of mucocutaneous, persistent ulcers on the external genitals. To identify carbohydrate receptors that mediate the attachment of this pathogen to host cells, we investigated the binding of 35S-methionine-labeled H. ducreyi strains to a panel of defined glycosphingolipids that were separated on thin layer chromatography plates. H. ducreyi bound to lactosylceramide, gangliotriaosylceramide, gangliotetraosylceramide, neolactotetraosylceramide, the GM3 ganglioside, and sulfatide. To elucidate the role of the surface-located 58.5-kDa GroEL heat shock protein (HSP) of H. ducreyi in attachment, we investigated the binding of purified HSP to the same panel of glycosphingolipids. Our results suggest that the 58.5-kDa GroEL HSP of H. ducreyi is responsible for the attachment of this bacterium to the majority of the tested glycosphingolipids, and thus represents a potential bacterial adhesin.

  2. Arsenophonus GroEL interacts with CLCuV and is localized in midgut and salivary gland of whitefly B. tabaci.

    Directory of Open Access Journals (Sweden)

    Vipin Singh Rana

    Full Text Available Cotton leaf curl virus (CLCuV (Gemininiviridae: Begomovirus is the causative agent of leaf curl disease in cotton plants (Gossypium hirsutum. CLCuV is exclusively transmitted by the whitefly species B. tabaci (Gennadius (Hemiptera: Alerodidae. B. tabaci contains several biotypes which harbor dissimilar bacterial endo-symbiotic community. It is reported that these bacterial endosymbionts produce a 63 kDa chaperon GroEL protein which binds to geminivirus particles and protects them from rapid degradation in gut and haemolymph. In biotype B, GroEL protein of Hamiltonella has been shown to interact with Tomato yellow leaf curl virus (TYLCV. The present study was initiated to find out whether endosymbionts of B. tabaci are similarly involved in CLCuV transmission in Sriganganagar (Rajasthan, an area endemic with cotton leaf curl disease. Biotype and endosymbiont diversity of B. tabaci were identified using MtCO1 and 16S rDNA genes respectively. Analysis of our results indicated that the collected B. tabaci population belong to AsiaII genetic group and harbor the primary endosymbiont Portiera and the secondary endosymbiont Arsenophonus. The GroEL proteins of Portiera and Arsenophonus were purified and in-vitro interaction studies were carried out using pull down and co-immunoprecipitation assays. In-vivo interaction was confirmed using yeast two hybrid system. In both in-vitro and in-vivo studies, the GroEL protein of Arsenophonus was found to be interacting with the CLCuV coat protein. Further, we also localized the presence of Arsenophonus in the salivary glands and the midgut of B. tabaci besides the already reported bacteriocytes. These results suggest the involvement of Arsenophonus in the transmission of CLCuV in AsiaII genetic group of B. tabaci.

  3. Arsenophonus GroEL interacts with CLCuV and is localized in midgut and salivary gland of whitefly B. tabaci.

    Science.gov (United States)

    Rana, Vipin Singh; Singh, Shalini Thakur; Priya, Natarajan Gayatri; Kumar, Jitendra; Rajagopal, Raman

    2012-01-01

    Cotton leaf curl virus (CLCuV) (Gemininiviridae: Begomovirus) is the causative agent of leaf curl disease in cotton plants (Gossypium hirsutum). CLCuV is exclusively transmitted by the whitefly species B. tabaci (Gennadius) (Hemiptera: Alerodidae). B. tabaci contains several biotypes which harbor dissimilar bacterial endo-symbiotic community. It is reported that these bacterial endosymbionts produce a 63 kDa chaperon GroEL protein which binds to geminivirus particles and protects them from rapid degradation in gut and haemolymph. In biotype B, GroEL protein of Hamiltonella has been shown to interact with Tomato yellow leaf curl virus (TYLCV). The present study was initiated to find out whether endosymbionts of B. tabaci are similarly involved in CLCuV transmission in Sriganganagar (Rajasthan), an area endemic with cotton leaf curl disease. Biotype and endosymbiont diversity of B. tabaci were identified using MtCO1 and 16S rDNA genes respectively. Analysis of our results indicated that the collected B. tabaci population belong to AsiaII genetic group and harbor the primary endosymbiont Portiera and the secondary endosymbiont Arsenophonus. The GroEL proteins of Portiera and Arsenophonus were purified and in-vitro interaction studies were carried out using pull down and co-immunoprecipitation assays. In-vivo interaction was confirmed using yeast two hybrid system. In both in-vitro and in-vivo studies, the GroEL protein of Arsenophonus was found to be interacting with the CLCuV coat protein. Further, we also localized the presence of Arsenophonus in the salivary glands and the midgut of B. tabaci besides the already reported bacteriocytes. These results suggest the involvement of Arsenophonus in the transmission of CLCuV in AsiaII genetic group of B. tabaci.

  4. "Kein Zeugnis abzulegen, wäre Verrat": Holocaustausstellungen an Stätten des Gedenkens im internationalen Vergleich – Deutschland, Frankreich, Großbritannien und Israel

    OpenAIRE

    Hofer-Englmaier, Iris Pamela Anna

    2015-01-01

    Die Studie zielt auf eine vergleichende Analyse der ausstellerischen Diskurse von Stätten des Gedenkens an den Holocaust in den Ländern Großbritannien, Frankreich, Israel und Deutschland. Hierbei wird der Begriff des Holocaust so weit gefasst, wie es im englischsprachigen Diskurs üblich ist. Er beinhaltet neben den Juden auch andere Bevölkerungsgruppen, die von den Nationalsozialisten verfolgt wurden. Untersucht werden konkret das Imperial War Museum, London's Museum of Jewish Life und d...

  5. Controversy of the GRO J1655-40 black hole mass and spin estimates and its possible solutions

    CERN Document Server

    Stuchlík, Zdeněk

    2016-01-01

    Estimates of the black hole mass $M$ and dimensionless spin $a$ in the microquasar GRO J1655-40 implied by strong gravity effects related to the timing and spectral measurements are controversial, if the mass restriction determined by the dynamics related to independent optical measurements, $M_{\\rm opt}=(5.4\\pm0.3) M_{\\odot}$, are applied. The timing measurements of twin high-frequency (HF) quasiperiodic oscillations (QPOs) with frequency ratio $3:2$ and the simultaneously observed low-frequency (LF) QPO imply the spin in the range $a\\in(0.27-0.29)$ if models based on the frequencies of the geodesic epicyclic motion are used to fit the timing measurements, and correlated creation of the twin HF QPOs and the LF QPO at a common radius is assumed. On the other hand, the spectral continuum method implies $a\\in(0.65-0.75)$, and the Fe-line-profile method implies $a\\in(0.94-0.98)$. This controversy can be cured, if we abandon the assumption of the occurrence of the twin HF QPOs and the simultaneously observed LF Q...

  6. Simultaneous NuSTAR/Chandra observations of the Bursting Pulsar GRO J1744-28 during its third reactivation

    CERN Document Server

    Younes, G; Grefenstette, B W; Tomsick, J A; Tennant, A; Finger, M H; Furst, F; Pottschmidt, K; Bhalerao, V; Boggs, S E; Boirin, L; Chakrabarty, D; Christensen, F E; Craig, W W; Degenaar, N; Fabian, A C; Gandhi, P; Gogus, E; Hailey, C J; Harrison, F A; Kennea, J A; Miller, J M; Stern, D; Zhang, W W

    2015-01-01

    We report on a 10 ks simultaneous Chandra/HETG-NuSTAR observation of the Bursting Pulsar, GRO J1744-28, during its third detected outburst since discovery and after nearly 18 years of quiescence. The source is detected up to 60 keV with an Eddington persistent flux level. Seven bursts, followed by dips, are seen with Chandra, three of which are also detected with NuSTAR. Timing analysis reveals a slight increase in the persistent emission pulsed fraction with energy (from 10% to 15%) up to 10 keV, above which it remains constant. The 0.5-70 keV spectra of the persistent and dip emission are the same within errors, and well described by a blackbody (BB), a power-law with an exponential rolloff, a 10 keV feature, and a 6.7 keV emission feature, all modified by neutral absorption. Assuming that the BB emission originates in an accretion disc, we estimate its inner (magnetospheric) radius to be about 4x10^7 cm, which translates to a surface dipole field B~9x10^10 G. The Chandra/HETG spectrum resolves the 6.7 keV ...

  7. Primjena nove tehnike prosušivanja bobica grožđa za proizvodnju prošeka

    OpenAIRE

    Sito, Stjepan; Peršurić Bernobić, Katarina; Bilandžija, Nikolina; Kraljević, Ante; Sinković, Ana; Sinković, Petra

    2013-01-01

    U radu su prikazani rezultati pokusnog prosušivanja grožđa sorti Muškat žuti i Muškat ruža crni dopremljenih iz vinograda blizu Poreča s OPG-a Peršurić Đordano. Uzorci prije sušenja sorte Muškat žuti imali su sadržaj šećera 87ºOe (186 g/L), a Muškat ruža crni 91ºOe (200 g/L). Nakon sušenja sadržaj šećera u soku bobica bio je oko 173ºOe (378 g/L). Proces prosušivanja uzoraka sorte Muškat žuti trajao je 120 sati, a kod sorte Muškat ruža crni 108 sati pri temperaturi zraka za sušenje od 35-38°C ...

  8. Die Facharztweiterbildung in Großbritannien oder die "Modernisierung der ärztlichen Karrieren" [Modernising Medical Careers

    Directory of Open Access Journals (Sweden)

    du Moulin, Marcel

    2007-05-01

    Full Text Available [english] This paper analyses the reform of postgraduate medical education introduced in the United Kingdom in 2003 under the heading "Modernising Medical Careers“. This nationally standardised and structured postgraduate medical training was started in 2005. The analysis includes the transition from undergraduate to postgraduate education, the new responsibilities in postgraduate education, the application process and the mode of distribution of the positions and the relationship of posts with and without postgraduate training opportunities. Possible consequences for postgraduate training and for the trainees are discussed. [german] Die Arbeit beschreibt und analysiert die Reform der Facharztweiterbildung in Großbritannien, die 2003 unter dem Titel "Modernising Medical Careers“ eingeführt wurde und seit 2005 umgesetzt wird. Vorgestellt werden der Übergang von der Ausbildung zur Weiterbildung, die neuen Zuständigkeiten für die Weiterbildung, das Auswahlverfahren und der Verteilungsmodus der Weiterbildungsstellen sowie das Verhältnis von Stellen mit und ohne Facharztperspektive. Die potenziellen Folgen für die Facharztweiterbildung und für die Weitergebildeten werden diskutiert.

  9. A preliminary in vitro assessment of GroBiotic-A, brewer's yeast and fructooligosaccharide as prebiotics for the red drum Sciaenops ocellatus.

    Science.gov (United States)

    Burr, Gary; Hume, Michael; Ricke, Steven; Nisbet, David; Gatlin, Delbert

    2008-01-01

    This study examined the effects of brewers yeast, fructooligosaccharide (FOS), and GroBiotic-A, a mixture of partially autolyzed brewers yeast, dairy components and dried fermentation products, on the intestinal microbial community of red drum, Sciaenops ocellatus. Gastrointestinal (GI) tracts were aseptically removed from three sub-adult red drum previously maintained on a commercial diet and placed in an anaerobic chamber. Intestinal contents were removed, diluted and incubated in vitro in one of four liquid media: normal diet alone, diet + 2% (w/w) GroBiotic-A, diet + 2% brewers yeast, and diet + 2% FOS. After 24 and 48 h of incubation at 25 degrees C, supernatants were removed for volatile fatty acid (VFA) analysis and DNA was extracted for denaturing gradient gel electrophoresis (DGGE) analysis. Polymerase chain reaction (PCR) was performed on a highly conserved region of M 16S rDNA and the amplicons were subjected to DGGE. The microbial community (MC) fingerprint was used to distinguish microbial populations. The intestinal contents incubated with GroBiotic-A had significantly (Pyeast.

  10. The Effects of Heterologous Expression of groEL from Arthrobacter simplex on the Resistance Performance of Escherichia coli%简单节杆菌groEL基因表达对大肠杆菌抗逆性能的影响

    Institute of Scientific and Technical Information of China (English)

    王艳霞; 王红玲; 王敏; 骆健美

    2016-01-01

    Arthrobacter simplex,a strain with steroid C1,2 dehydrogenation ability,has been widely used in the steroid industry, usually adding ethanol in the transforming system may catalyze the dissolution of hydrophobic substrates. Our prior work by LC-MS/MS and bioinformatics analysis found that heat shock protein(HSP)GroEL was closely correlated to the cell adaptive behavior under ethanol stress condition. Based on this concept,groEL gene was cloned by PCR while usingA. simplexTCCC 11037 genome as a template. The results showed that the homologies of nucleotide and amino acid sequence of GroEL in the strain were the highest with that fromNocardioides sp. JS614 by the ratio of 89% and 95%,respectively. Then its heterologous expression was conducted in Escherichia coli BL21(DE3)with the vector pET-22b (+),and the results showed that the recombinant strain presented the significant increase on the survival performance under the shock condition with high-concentration of methanol,hypertonicity and superacid,as well as the growth performance under the proper stresses of them. The study was the first report on the cloning and expression of genegroEL fromA. simplex,and also provided fundamental data for exploring the biological function of HSP protein GroEL from A. simplex.%以简单节杆菌(Arthrobacter simplex)TCCC 11037基因组为模板,通过PCR方法进行groEL基因克隆,结果表明,该菌株中GroEL的核苷酸和氨基酸序列与Nocardioidessp. JS614来源的GroEL同源性最高,分别为89%和95%。通过pET-22b(+)将其在大肠杆菌BL21(DE3)中进行异源表达,结果表明,重组菌株在甲醇、高渗和强酸这3种压力高浓度冲击条件下的存活能力和适当压力条件下的生长性能均明显提高。

  11. ThegroE operon ofStreptococcus mutans with its expression and regulation%变异链球菌groE操纵子及其表达与调控

    Institute of Scientific and Technical Information of China (English)

    王一舟; 张雅琪; 牛雪微; 张志民

    2016-01-01

    变异链球菌耐受口腔内多种环境的变化,主要依赖于多种热休克蛋白基因的表达。其中,groE操纵子表达的GroES-GroEL蛋白可辅助新合成的以及变性的蛋白质折叠、组装、转运和降解,从而影响细胞的代谢。变异链球菌groE操纵子位于1 834 692~1 832 649位点,包括σA型启动子、分子伴侣表达反向重复序列(CIRCE)、groES和groEL及终止子,在进化上高度保守,其表达受热、酸、乙醇和过氧化氢等多种应激环境的诱导,受CtsR和HrcA蛋白的双重负性调节。groE操纵子的调控有HrcA-CIRCE系统负调控假说和CtsR的负调控两种方式,但其具体调控机制尚未在变异链球菌中得到充分证实。从分子水平上研究变异链球菌groE操纵子的结构和调控机制,有助于进一步阐明细胞的生理过程,为了解细胞在应激和病变状态下的分子调节机制打下基础。%Streptococcus mutans(S.mutans), as one of the primary cariogenic bacteria, can respond to several environmental stresses. This ability mainly depends on the translation and expression of variety of heat shock protein genes. groE operon, one of the best-studied heat shock genes, affects the metabolism of cells by translating the heat shock proteins, groES-groEL, which can mediate the folding, assembly, transport, and degradation of new or misfolding proteins. The groE operon locates in 1 834 692-1 832 649 sites, including a σA promoter, inverted repeat sequence(CIRCE), groES, groEL and a terminator. It is highly conserved, and can be induced to express by stress environment including heat, acid, ethanol and hydrogen peroxide. Both HrcA-CIRCE system and CtsR play a negative regulation role, without a clear mechanism. Studies, about the structure and regulation mechanism of S.mutans groE operon in a molecular level, help to further clarify the physiological process of cells, and lay the foundation for understanding the molecular

  12. Controversy of the GRO J1655-40 Black Hole Mass and Spin Estimates and Its Possible Solutions

    Science.gov (United States)

    Stuchlík, Z.; Kološ, M.

    2016-07-01

    Estimates of the black hole mass M and the dimensionless spin a in the microquasar GRO J1655-40 implied by strong gravity effects related to the timing and spectral measurements are controversial, if the mass restriction determined by the dynamics related to independent optical measurements, M opt = (5.4 ± 0.3) M ⊙, is applied. The timing measurements of twin high-frequency (HF) quasiperiodic oscillations (QPOs) with the frequency ratio 3:2 and the simultaneously observed low-frequency (LF) QPO imply spin in the range a\\in (0.27{--}0.29) if models based on the frequencies of geodesic epicyclic motion are used to fit the timing measurements, and the correlated creation of the twin HF QPOs and the LF QPO at a common radius is assumed. On the other hand, the spectral continuum method implies a\\in (0.65{--}0.75), and the Fe-line-profile method implies a\\in (0.94{--}0.98). This controversy can be cured if we abandon the assumption of the occurrence of the twin HF QPOs and the simultaneously observed LF QPO at a common radius. We demonstrate that the epicyclic resonance model of the twin HF QPOs is able to predict the spin in agreement with the Fe-profile method, but no model based on the geodesic epicyclic frequencies can be in agreement with the spectral continuum method. We also show that the non-geodesic string loop oscillation model of twin HF QPOs predicts spin a > 0.3 under the optical measurement limit on the black hole mass, which is in agreement with both the spectral continuum and Fe-profile methods.

  13. Simultaneous NuSTAR/Chandra Observations of the Bursting Pulsar GRO J1744-28 during Its Third Reactivation

    Science.gov (United States)

    Younes, G.; Kouveliotou, C.; Grefenstette, B. W.; Tomsick, J. A.; Tennant, A.; Finger, M. H.; Fürst, F.; Pottschmidt, K.; Bhalerao, V.; Boggs, S. E.; Boirin, L.; Chakrabarty, D.; Christensen, F. E.; Craig, W. W.; Degenaar, N.; Fabian, A. C.; Gandhi, P.; Göğüş, E.; Hailey, C. J.; Harrison, F. A.; Kennea, J. A.; Miller, J. M.; Stern, D.; Zhang, W. W.

    2015-05-01

    We report on a 10 ks simultaneous Chandra/High Energy Transmission Grating (HETG)-Nuclear Spectroscopic Telescope Array (NuSTAR) observation of the Bursting Pulsar, GRO J1744-28, during its third detected outburst since discovery and after nearly 18 yr of quiescence. The source is detected up to 60 keV with an Eddington persistent flux level. Seven bursts, followed by dips, are seen with Chandra, three of which are also detected with NuSTAR. Timing analysis reveals a slight increase in the persistent emission pulsed fraction with energy (from 10% to 15%) up to 10 keV, above which it remains constant. The 0.5-70 keV spectra of the persistent and dip emission are the same within errors and well described by a blackbody (BB), a power-law (PL) with an exponential rolloff, a 10 keV feature, and a 6.7 keV emission feature, all modified by neutral absorption. Assuming that the BB emission originates in an accretion disk, we estimate its inner (magnetospheric) radius to be about 4 × 107 cm, which translates to a surface dipole field B ≈ 9 × 1010 G. The Chandra/HETG spectrum resolves the 6.7 keV feature into (quasi-)neutral and highly ionized Fe xxv and Fe xxvi emission lines. XSTAR modeling shows these lines to also emanate from a truncated accretion disk. The burst spectra, with a peak flux more than an order of magnitude higher than Eddington, are well fit with a PL with an exponential rolloff and a 10 keV feature, with similar fit values compared to the persistent and dip spectra. The burst spectra lack a thermal component and any Fe features. Anisotropic (beamed) burst emission would explain both the lack of the BB and any Fe components.

  14. SIMULTANEOUS NuSTAR/CHANDRA OBSERVATIONS OF THE BURSTING PULSAR GRO J1744-28 DURING ITS THIRD REACTIVATION

    Energy Technology Data Exchange (ETDEWEB)

    Younes, G.; Finger, M. H. [Universities Space Research Association, 6767 Old Madison Pike, Suite 450, Huntsville, AL 35806 (United States); Kouveliotou, C.; Tennant, A. [Astrophysics Office, ZP 12, NASA-Marshall Space Flight Center, Huntsville, AL 35812 (United States); Grefenstette, B. W.; Fürst, F. [Cahill Center for Astrophysics, 1216 East California Boulevard, California Institute of Technology, Pasadena, CA 91125 (United States); Tomsick, J. A.; Boggs, S. E.; Craig, W. W. [Space Sciences Laboratory, 7 Gauss Way, University of California, Berkeley, CA 94720-7450 (United States); Pottschmidt, K. [Center for Space Science and Technology, University of Maryland Baltimore County, Baltimore, MD 21250 (United States); Bhalerao, V. [Inter-University Center for Astronomy and Astrophysics, Post Bag 4, Ganeshkhind, Pune 411007 (India); Boirin, L. [Observatoire Astronomique de Strasbourg, 11 Rue de l' Université, F-67000 Strasbourg (France); Chakrabarty, D. [Kavli Institute for Astrophysics and Space Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Christensen, F. E. [DTU Space, National Space Institute, Technical University of Denmark, Elektrovej 327, DK-2800 Lyngby (Denmark); Degenaar, N. [Department of Astronomy, University of Michigan, 1085 South University Avenue, Ann Arbor, MI 48109 (United States); Fabian, A. C. [Institute of Astronomy, Madingley Road, Cambridge CB3 0HA (United Kingdom); Gandhi, P. [Department of Physics, Durham University, South Road, Durham DH1 3LE (United Kingdom); Göğüş, E. [Sabancı University, Orhanlı-Tuzla, İstanbul 34956 (Turkey); and others

    2015-05-01

    We report on a 10 ks simultaneous Chandra/High Energy Transmission Grating (HETG)–Nuclear Spectroscopic Telescope Array (NuSTAR) observation of the Bursting Pulsar, GRO J1744-28, during its third detected outburst since discovery and after nearly 18 yr of quiescence. The source is detected up to 60 keV with an Eddington persistent flux level. Seven bursts, followed by dips, are seen with Chandra, three of which are also detected with NuSTAR. Timing analysis reveals a slight increase in the persistent emission pulsed fraction with energy (from 10% to 15%) up to 10 keV, above which it remains constant. The 0.5–70 keV spectra of the persistent and dip emission are the same within errors and well described by a blackbody (BB), a power-law (PL) with an exponential rolloff, a 10 keV feature, and a 6.7 keV emission feature, all modified by neutral absorption. Assuming that the BB emission originates in an accretion disk, we estimate its inner (magnetospheric) radius to be about 4 × 10{sup 7} cm, which translates to a surface dipole field B ≈ 9 × 10{sup 10} G. The Chandra/HETG spectrum resolves the 6.7 keV feature into (quasi-)neutral and highly ionized Fe xxv and Fe xxvi emission lines. XSTAR modeling shows these lines to also emanate from a truncated accretion disk. The burst spectra, with a peak flux more than an order of magnitude higher than Eddington, are well fit with a PL with an exponential rolloff and a 10 keV feature, with similar fit values compared to the persistent and dip spectra. The burst spectra lack a thermal component and any Fe features. Anisotropic (beamed) burst emission would explain both the lack of the BB and any Fe components.

  15. INTEGRAL Galactic bulge monitoring observations of GRO J1750-27 (AX J1749.1-2639), H1743-322 and SLX 1746-331

    DEFF Research Database (Denmark)

    Kuulkers, E.; Beckmann, V.; Shaw, S.;

    2008-01-01

    A new season of the INTEGRAL Galactic Bulge monitoring program (see ATels #438, #874, #1005; Kuulkers et al. 2007, A&A 466, 595) started, with observations on UT 11 Feb 2008, 16:33-18:07. We here report on results from three currently active transient sources. The IBIS/ISGRI and JEM-X1 images sho...... a bright source near GX 3+1, coincident with the position of the transient accreting X-ray pulsar GRO J1750-27 (AX J1749.1-2639)....

  16. Strontium-Substituted Bioceramics Particles: A New Way to Modulate MCP-1 and Gro-α Production by Human Primary Osteoblastic Cells

    Directory of Open Access Journals (Sweden)

    Julien Braux

    2016-12-01

    Full Text Available Background: To avoid morbidity and limited availability associated with autografts, synthetic calcium phosphate (CaP ceramics were extensively developed and used as bone filling materials. Controlling their induced-inflammatory response nevertheless remained a major concern. Strontium-containing CaP ceramics were recently demonstrated for impacting cytokines’ secretion pattern of human primary monocytes. The present study focuses on the ability of strontium-containing CaP to control the human primary bone cell production of two major inflammatory and pro-osteoclastogenic mediators, namely MCP-1 and Gro-α, in response to ceramics particles. Methods: This in vitro study was performed using human primary osteoblasts in which their response to ceramics was evaluated by PCR arrays, antibody arrays were used for screening and real-time PCR and ELISA for more focused analyses. Results: Study of mRNA and protein expression highlights that human primary bone cells are able to produce these inflammatory mediators and reveal that the adjunction of CaP in the culture medium leads to their enhanced production. Importantly, the current work determines the down-regulating effect of strontium-substituted CaP on MCP-1 and Gro-α production. Conclusion: Our findings point out a new capability of strontium to modulate human primary bone cells’ communication with the immune system.

  17. INTEGRAL detection of the on-going outbursts from 1RXS J180408.9-342058 and GRO J1750-27

    DEFF Research Database (Denmark)

    Boissay, R.; Chenevez, J.; Wilms, J.

    2015-01-01

    During the observations of the Galactic Center (from 2015 February 16 12:46 to February 17 at 4:25 UTC), INTEGRAL detected the two sources 1RXS J180408.9-342058 (Atel #6997, #7008, #7039) and GRO J1750-27 (Atel #1385, #1400, #6839) which were recently reported to undergo new outbursts. 1RXS J180408.......9-342058 was observed in both the IBIS/ISGRI and the JEM-X FoV. A preliminary analysis of the ISGRI and JEM-X data revealed that the source broad-band spectrum (effective exposure time 34.2 ks for ISGRI and 9.3 ks for the two JEM-X) could be described by using a cut-off power-law model. We measured a power-law photon...... of the source. GRO J1750-27 was observed by IBIS/ISGRI for an effective exposure time 34.2 ks and by the two JEM-X for a total effective exposure time 9 ks. The ISGRI spectrum of the source could be roughly described with a soft power-law of photon index ~4. The estimated source flux from the spectral fit is 7...

  18. Application of a relativistic accretion disc model to X-ray spectra of LMC X-1 and GRO J1655-40

    CERN Document Server

    Gierlinski, M; Ebisawa, K; Gierlinski, Marek; Maciolek-Niedzwiecki, Andrzej; Ebisawa, Ken

    2001-01-01

    We present a general relativistic accretion disc model and its application to the soft-state X-ray spectra of black hole binaries. The model assumes a flat, optically thick disc around a rotating Kerr black hole. The disc locally radiates away the dissipated energy as a blackbody. Special and general relativistic effects influencing photons emitted by the disc are taken into account. The emerging spectrum, as seen by a distant observer, is parametrized by the black hole mass and spin, the accretion rate, the disc inclination angle and the inner disc radius. We fit the ASCA soft state X-ray spectra of LMC X-1 and GRO J1655-40 by this model. We find that having additional limits on the black hole mass and inclination angle from optical/UV observations, we can constrain the black hole spin from X-ray data. In LMC X-1 the constrain is weak, we can only rule out the maximally rotating black hole. In GRO J1655-40 we can limit the spin much better, and we find 0.68 < a < 0.88. Accretion discs in both sources a...

  19. Development of PCR primers and a DNA macroarray for the simultaneous detection of major Staphylococcus species using groESL gene.

    Science.gov (United States)

    Chiang, Yu-Cheng; Lu, Hsi-Chi; Li, Sheng-Chih; Chang, Yu-Hsin; Chen, Hsin-Yen; Lin, Chia-Wei; Tsen, Hau-Yang

    2012-03-01

    Staphylococcus spp., including S. aureus, S. intermedius, S. hyicus, S. epidermidis, S. saprophyticus, S. haemolyticus, S. xylosus, and S. carnosus, are major bacterial species associated with food poisoning, and human and veterinary clinics. Traditional methods for the identification of these staphylococci are time-consuming, laborious, or inaccurate. Therefore, rapid and accurate diagnostic methods are needed. In this study, we designed the DNA probes and polymerase chain reaction (PCR) primers for the detection of the aforementioned Staphylococcus species. These primers were proved to be specific for the detection of their corresponding target strains. Furthermore, by using a consensus primer pair, we were able to co-amplify the intergenic region of groES-groEL for these staphylococci. Followed by a chromogenic macroarray system with the specific probes on the plastic chips, these staphylococci in milk products or clinical samples could be simultaneously detected. When the system was used for the inspection of milk or urine samples containing N × 10⁰ target cells per milliliter of the sample, all these staphylococcal species could be identified after an 8-h pre-enrichment step. This system also allowed the adequate diagnosis of bacteremia, since N × 10⁰ target cells per milliliter of the blood samples could be detected after a 12-h pre-enrichment. Compared to the multiplex PCR method, this approach has the additional advantage that it allowed the discrimination of more bacterial strains-even some bacterial strains that may generate PCR products with the same molecular sizes.

  20. A key role for early growth response-1 and nuclear factor-kappaB in mediating and maintaining GRO/CXCR2 proliferative signaling in esophageal cancer.

    Science.gov (United States)

    Wang, Bo; Khachigian, Levon M; Esau, Luke; Birrer, Michael J; Zhao, Xiaohang; Parker, M Iqbal; Hendricks, Denver T

    2009-05-01

    Although early growth response-1 (EGR-1) has been shown as a key transcription factor in controlling cell growth, proliferation, differentiation, and angiogenesis, its role in the development of esophageal cancer is poorly understood despite the high frequency of this disease in many parts of the world. Here, immunohistochemistry showed that EGR-1 is overexpressed in 80% of esophageal tumor tissues examined. Furthermore, EGR-1 is constitutively expressed in all esophageal cancer cell lines analyzed. Esophageal squamous carcinoma WHCO1 cells stably transfected with EGR-1 short hairpin RNA displayed a 55% reduction in EGR-1 protein levels, 50% reduction in cell proliferation, a 50% reduction in cyclin-dependent kinase 4 levels, and a 2-fold induction in p27(Kip1) levels associated with a G(2)-M cell cycle arrest. EGR-1 knockdown also caused a marked induction in IkappaBalpha expression, an effect also observed in GRObeta RNA interference-expressing WHCO1 cells, because EGR-1 lies downstream of GRO/CXCR2 signaling. Furthermore, p65 mRNA levels were also reduced in cells treated with either short hairpin RNA EGR-1 or small interfering RNA EGR-1. Immunohistochemical analysis indicated that p65 is elevated in 78% (n = 61) of esophageal tumor sections analyzed. Moreover, nuclear factor-kappaB inhibition with either sodium salicylate or p65 RNA interference led to a significant reduction in GROalpha and GRObeta expression. These results indicate that EGR-1 and nuclear factor-kappaB mediate GRO/CXCR2 proliferative signaling in esophageal cancer and may represent potential target molecules for therapeutic intervention.

  1. The immobilization of heavy metals in soil by bioaugmentation of a UV-mutant Bacillus subtilis 38 assisted by NovoGro biostimulation and changes of soil microbial community

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Ting [MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin 300071 (China); Urban Transport Emission Control Research Centre, College of Environmental Science and Engineering, Nankai University, Tianjin 300071 (China); Sun, Hongwen, E-mail: sunhongwen@nankai.edu.cn [MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin 300071 (China); Mao, Hongjun [Urban Transport Emission Control Research Centre, College of Environmental Science and Engineering, Nankai University, Tianjin 300071 (China); Zhang, Yanfeng; Wang, Cuiping; Zhang, Zhiyuan; Wang, Baolin; Sun, Lei [MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin 300071 (China)

    2014-08-15

    Highlights: • A UV-mutated species, Bacillus subtilis 38, is a good sorbent for multi-metals (Cd, Cr, Hg and Pb). • B38 mixed with NovoGro exhibited a synergetic effect on the immobilization of heavy metals in soil. • DTPA, M3 and BCR were suitable for predicting metal bioavailability for specific classes of plant. • The NovoGro could enhance the proliferation of both exotic B38 and native microbes. • It's a practical strategy for the remediation of actual farmland polluted by multi-heavy metals. - Abstract: Bacillus subtilis 38 (B38) is a mutant species of Bacillus subtilis acquired by UV irradiation with high cadmium tolerance. This study revealed that B38 was a good biosorbent for the adsorption of multiple heavy metals (cadmium, chromium, mercury, and lead). Simultaneous application of B38 and NovoGro (SNB) exhibited a synergetic effect on the immobilization of heavy metals in soil. The heavy metal concentrations in the edible part of the tested plants (lettuce, radish, and soybean) under SNB treatment decreased by 55.4–97.9% compared to the control. Three single extraction methods, diethylenetriaminepentaacetic acid (DTPA), Mehlich 3 (M3), and the first step of the Community Bureau of Reference method (BCR1), showed good predictive capacities for metal bioavailability to leafy, rhizome, and leguminous plant, respectively. The polymerase chain reaction–denaturing gradient gel electrophoresis (PCR–DGGE) profiles revealed that NovoGro could enhance the proliferation of both exotic B38 and native microbes. Finally, the technology was checked in the field, the reduction in heavy metal concentrations in the edible part of radish was in the range between 30.8% and 96.0% after bioremediation by SNB treatment. This study provides a practical strategy for the remediation of farmland contaminated by multiple heavy metals.

  2. Wind, jet, hybrid corona and hard X-ray flares: multiwavelength evolution of GRO J1655-40 during the 2005 outburst rise

    CERN Document Server

    Kalemci, E; Maccarone, T J; Dincer, T; Russell, T D; Bailyn, C; Tomsick, J A

    2016-01-01

    We have investigated the complex multiwavelength evolution of GRO J1655-40 during the rise of its 2005 outburst. We detected two hard X-ray flares, the first one during the transition from the soft state to the ultra-soft state, and the second one in the ultra-soft state. The first X-ray flare coincided with an optically thin radio flare. We also observed a hint of increased radio emission during the second X-ray flare. To explain the hard flares without invoking a secondary emission component, we fit the entire data set with the eqpair model. This single, hybrid Comptonization model sufficiently fits the data even during the hard X-ray flares if we allow reflection fractions greater than unity. In this case, the hard X-ray flares correspond to a Comptonizing corona dominated by non-thermal electrons. The fits also require absorption features in the soft and ultra-soft state which are likely due to a wind. In this work we show that the wind and the optically thin radio flare co-exist. Finally, we have also in...

  3. Using GRO J1655-40 to test Swift/BAT as a monitor for bright hard X-ray sources

    CERN Document Server

    Senziani, F; De Luca, A; Caraveo, P A; Belloni, T; Bignami, Giovanni Fabrizio

    2007-01-01

    While waiting for new gamma-ray burst detections, the Burst Alert Telescope (BAT) on board Swift covers each day ~50% of the sky in the hard X-ray band (``Survey data''). The large field of view (FOV), high sensitivity and good angular resolution make BAT a potentially powerful all-sky hard X-ray monitor, provided that mask--related systematics can be properly accounted for. We have developed and tested a complete procedure entirely based on public Swift/BAT software tools to analyse BAT Survey data, aimed at assessing the flux and spectral variability of bright sources in the 15-150 keV energy range. Detailed tests of the capabilities of our procedure were performed focusing, in particular, on the reliability of spectral measurements over the entire BAT FOV. First, we analyzed a large set of Crab observations, spread over ~7 months. Next, we studied the case of GRO J1655-40, a strongly variable source, which experienced a 9-month long outburst, beginning on February 2005. Such an outburst was systematically ...

  4. A target-oriented magnetotelluric inversion approach for characterizing the low enthalpy Groß Schönebeck geothermal reservoir

    Science.gov (United States)

    Muñoz, G.; Ritter, O.; Moeck, I.

    2010-12-01

    Electrical conductivity is a key parameter for the exploration and characterization of geothermal reservoirs as hot mineralized formation water of active geothermal areas usually exhibits significantly higher conductivity than the surrounding host rock. Here we present results of a magnetotelluric (MT) exploration experiment carried out in the vicinity of the Groß Schönebeck geothermal test site in Northern Germany, where a doublet system of two 4.3-km deep boreholes was drilled to establish an in situ laboratory to investigate the potential for geothermal energy production. Classical 2-D smooth inversion of the MT data, recorded along two profiles, reveals a shallow conductive structure in good agreement with information from regional geology and seismic images. However, at the northernmost part of the profiles, the conductivity models reveal deep-reaching conductive structures, which appear uncorrelated with existing (geophysical or geological) data. Incorporating information from seismics as independent constraints for MT inversions allows us to examine the model space rigorously but target oriented. Employing so-called tear-zone inversions we can effectively derive an alternative class of models, which are consistent with the MT observations but also with the other data sets. We speculate that the zones of high conductivity imaged at reservoir depth are related areas of reduced thickness of the overlaying evapourite layer. The enhanced conductivity can be explained by a higher fracture density in anhydritic layers and/or generally lower resistivity of the pore fluid.

  5. Evidence for a change in the radiation mechanism in the hard state of GRO J1655-40. Hysteresis in the broad-band noise components

    CERN Document Server

    Reig, P; Sobolewska, M A; Malzac, J

    2013-01-01

    We have analysed RXTE archival data to study the aperiodic variability of the black-hole binary GRO J1655-40 during the hard state of the 2005 outburst. We computed the 0.008-64 Hz power spectral density during the rise and decay of the 2005 outburst, and we found that they were reasonably well modelled by the sum of two, broad Lorenztian functions in most cases (plus a narrow QPO) which correspond to three different variability components. Our aim is to study the evolution of the timing properties of the source during the outburst, by studying the correlation between the characteristics of the broad-band noise components in the power spectra and the source luminosity. Our results suggest that the whole power spectrum shifts to high (low) frequencies as the source luminosity increases (decreases), in agreement with previous studies of other black hole binaries. However, we also detect a strong "hysteresis" pattern in the "frequency-luminosity" plots, and show that the "critical" luminosity limit, above which ...

  6. Wind, jet, hybrid corona and hard X-ray flares: multiwavelength evolution of GRO J1655-40 during the 2005 outburst rise

    Science.gov (United States)

    Kalemci, E.; Begelman, M. C.; Maccarone, T. J.; Dinçer, T.; Russell, T. D.; Bailyn, C.; Tomsick, J. A.

    2016-11-01

    We have investigated the complex multiwavelength evolution of GRO J1655-40 during the rise of its 2005 outburst. We detected two hard X-ray flares, the first one during the transition from the soft state to the ultra-soft state, and the second one in the ultra-soft state. The first X-ray flare coincided with an optically thin radio flare. We also observed a hint of increased radio emission during the second X-ray flare. To explain the hard flares without invoking a secondary emission component, we fit the entire data set with the eqpair model. This single, hybrid Comptonization model sufficiently fits the data even during the hard X-ray flares if we allow reflection fractions greater than unity. In this case, the hard X-ray flares correspond to a Comptonizing corona dominated by non-thermal electrons. The fits also require absorption features in the soft and ultra-soft state which are likely due to a wind. In this work we show that the wind and the optically thin radio flare co-exist. Finally, we have also investigated the radio to optical spectral energy distribution, tracking the radio spectral evolution through the quenching of the compact jet and rise of the optically thin flare, and interpreted all data using state transition models.

  7. Extended abstracts from the eight international meeting on progress in radio-oncology (ICRO/OeGRO 8), Salzburg, May 16-19, 2007

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2007-12-15

    This part of the journal includes 25 extended abstracts from the Eight International Meeting on Progress in Radio-Oncology ICRO/OeGRO8 in Salzburg, Austria (May 16-19, 2007): adaptive radiotherapy of prostate cancer; homoradiation of prostate cancer; radiation therapy, chemotherapy and combined chemoradiation in the management of malignant glioma; return of high-dose per fraction radiotherapy: paradigm change, lesson from the past; from bench to bedside: enhancing the effects of radiotherapy through biological escalation; gastric cancer: MAGIC or Macdonald; analysis of set-up errors in 70 consecutive patients treated for rectal carcinoma; molecular targeted therapy; teleradiooncology: telematics applications in therapeutic radiology and oncology; in-room guidance technologies for adaptive radiotherapy; dose escalation with photons - emerging technologies; late side-effects after pelvic irradiation; a frameless robotic stereotactic system for highly focused extracranial radiotherapy; altered fractionation in head and neck tumors: an alternative to chemotherapy; integration of molecular targeting with radiotherapy; OGRT pf prostate cancer patients based on CBCT and kV images; hyperfractionation in medulloblastoma; re-irradiation of recurrent prostate cancer; dose reduction of radiotherapy in early-stage testicular semimoma; photons versus protons; randomized clinical trials on postoperative radiotherapy for high-risk head and neck cancer; cardiac risk in multimodal breast cancer treatment; the use of hyperthermia treatment planning in clinical practice; presentation of the ICRU-IAEA Joint Report 'prescribing, recording, and reporting proton-beam therapy'.

  8. Co-administration of rIpaB domain of Shigella with rGroEL of S. Typhi enhances the immune responses and protective efficacy against Shigella infection.

    Science.gov (United States)

    Chitradevi, Sekar Tamil Selvi; Kaur, Gurpreet; Uppalapati, Sivaramakrishna; Yadav, Anandprakash; Singh, Dependrapratap; Bansal, Anju

    2015-11-01

    Shigella species cause severe bacillary dysentery in humans and are associated with high morbidity and mortality. The Invasion plasmid antigen (IpaB) protein, which is conserved across all Shigella spp., induces macrophage cell death and is required to invade host cells. The present study evaluates the immunogenicity and protective efficacy of the recombinant (r) domain region of IpaB (rIpaB) of S. flexneri. rIpaB was administered either alone or was co-administered with the rGroEL (heat shock protein 60) protein from S. Typhi as an adjuvant in a mouse model of intranasal immunization. The IpaB domain region (37 kDa) of S. flexneri was amplified from an invasion plasmid, cloned, expressed in BL21 Escherichia coli cells and purified. Immunization with the rIpaB domain alone stimulated both humoral and cell-mediated immune responses. Furthermore, robust antibody (IgG, IgA) and T-cell responses were induced when the rIpaB domain was co-administered with rGroEL. Antibody isotyping revealed higher IgG1 and IgG2a antibody titers and increased interferon-gamma (IFN-γ) secretion in the co-administered group. Immunization of mice with the rIpaB domain alone protected 60%-70% of the mice from lethal infection by S. flexneri, S. boydii and S. sonnei, whereas co-administration with rGroEL increased the protective efficacy to 80%-85%. Organ burden and histopathological studies also revealed a significant reduction in lung infection in the co-immunized mice compared with mice immunized with the rIpaB domain alone. This study emphasizes that the co-administration of the rIpaB domain and rGroEL protein improves immune responses in mice and increases protective efficacy against Shigella infection. This is also the first report to evaluate the potential of the GroEL (Hsp 60) protein of S. Typhi as an adjuvant molecule, thereby overcoming the need for commercial adjuvants.

  9. Thermo-poroelastic numerical modelling for enhanced geothermal system performance: Case study of the Groß Schönebeck reservoir

    Science.gov (United States)

    Jacquey, Antoine B.; Cacace, Mauro; Blöcher, Guido; Watanabe, Norihiro; Huenges, Ernst; Scheck-Wenderoth, Magdalena

    2016-08-01

    Significant pressure and temperature changes can occur within geothermal reservoirs caused by injection and production of fluid which can affect transport properties of the rocks and therefore alter reservoir performance and sustainability. To understand the coupling between transport properties evolution and state variable changes, a complete description of the mechanical behavior of the reservoir is required which should consider thermo- and poroelastic effects. This study aims to integrate transport properties evolution for coupled thermo-hydro-mechanical (THM) process modelling of fluid-bearing reservoirs. This approach is here applied to the geothermal research site of Groß Schönebeck (40 km north of Berlin, Germany) which consists of a doublet system at a target depth of about - 4100 m in which both injection and production wells have been hydraulically stimulated. A 3D reservoir model including the main geological units, major natural fault zones and hydraulic fractures is integrated in the finite-element method-based simulator OpenGeoSys for modelling coupled THM processes during geothermal activity. One challenge of this study is to integrate both hydro-geological and physical complexity to better describe the dynamic behavior of the geothermal reservoir. From the results of the simulation, thermal breakthrough is observed after 18 years of injection and life time of the system has been evaluated as 50 years. Furthermore, a 5.5% increase of porosity around the injection well is observed as well as an increase of the anisotropy ratio for permeability (kz/kxy) of about 2%. These transport properties enhancements lead to a decrease of the thermal breakthrough time (around - 8%) and life time of the system (- 14%) compared to classic thermo-hydro simulations with constant transport properties. The results presented here provide therefore valuable insights for understanding porosity and permeability distributions and evolutions during injection and production

  10. Comparison of the nucleotide sequences of 16S rRNA, 444 Ep-ank, and groESL heat shock operon genes in naturally occurring Ehrlichia equi and human granulocytic ehrlichiosis agent isolates from Northern California.

    Science.gov (United States)

    Chae, J S; Foley, J E; Dumler, J S; Madigan, J E

    2000-04-01

    We examined 11 naturally occurring isolates of Ehrlichia equi in horses and two human granulocytic ehrlichiosis agent isolates in California for sequence diversity in three genes. Ehrlichia equi isolates were from Sierra (n = 6), Mendocino (n = 3), Sonoma (n = 1), and Marin (n = 1) counties, and human granulocytic ehrlichiosis (HGE) agent isolates were obtained from Humboldt county. PCR with specific primers for 16S rRNA, 444 Ep-ank and groESL heat shock operon genes successfully produced amplicons for all 13 clinical samples. The 444 Ep-ank gene of the HGE agent and E. equi isolates from northern California is different from the eastern U.S. isolates BDS and USG3. The translated amino acid sequence of the groESL heat shock operon gene fragment is identical among E. equi, the HGE agent, and E. phagocytophila, with the exception of the northern Californian equine CASOLJ isolate. Microheterogeneity was observed in the 16S rRNA gene sequences of HGE agent and E. equi isolates from northern California. These results suggest that E. equi and the HGE agent found in California are similar or identical but may differ from the isolates of equine and human origin found in the eastern United States.

  11. The immobilization of heavy metals in soil by bioaugmentation of a UV-mutant Bacillus subtilis 38 assisted by NovoGro biostimulation and changes of soil microbial community.

    Science.gov (United States)

    Wang, Ting; Sun, Hongwen; Mao, Hongjun; Zhang, Yanfeng; Wang, Cuiping; Zhang, Zhiyuan; Wang, Baolin; Sun, Lei

    2014-08-15

    Bacillus subtilis 38 (B38) is a mutant species of Bacillus subtilis acquired by UV irradiation with high cadmium tolerance. This study revealed that B38 was a good biosorbent for the adsorption of multiple heavy metals (cadmium, chromium, mercury, and lead). Simultaneous application of B38 and NovoGro (SNB) exhibited a synergetic effect on the immobilization of heavy metals in soil. The heavy metal concentrations in the edible part of the tested plants (lettuce, radish, and soybean) under SNB treatment decreased by 55.4-97.9% compared to the control. Three single extraction methods, diethylenetriaminepentaacetic acid (DTPA), Mehlich 3 (M3), and the first step of the Community Bureau of Reference method (BCR1), showed good predictive capacities for metal bioavailability to leafy, rhizome, and leguminous plant, respectively. The polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profiles revealed that NovoGro could enhance the proliferation of both exotic B38 and native microbes. Finally, the technology was checked in the field, the reduction in heavy metal concentrations in the edible part of radish was in the range between 30.8% and 96.0% after bioremediation by SNB treatment. This study provides a practical strategy for the remediation of farmland contaminated by multiple heavy metals. Copyright © 2014 Elsevier B.V. All rights reserved.

  12. Phospholipase D from Loxosceles laeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration

    Directory of Open Access Journals (Sweden)

    José M. Rojas

    2017-04-01

    Full Text Available Cutaneous loxoscelism envenomation by Loxosceles spiders is characterized by the development of a dermonecrotic lesion, strong inflammatory response, the production of pro-inflammatory mediators, and leukocyte migration to the bite site. The role of phospholipase D (PLD from Loxosceles in the recruitment and migration of monocytes to the envenomation site has not yet been described. This study reports on the expression and production profiles of cytokines and chemokines in human skin fibroblasts treated with catalytically active and inactive recombinant PLDs from Loxosceles laeta (rLlPLD and lipid inflammatory mediators ceramide 1-phosphate (C1P and lysophosphatidic acid (LPA, and the evaluation of their roles in monocyte migration. Recombinant rLlPLD1 (active and rLlPLD2 (inactive isoforms induce interleukin (IL-6, IL-8, CXCL1/GRO-α, and CCL2/monocyte chemoattractant protein-1 (MCP-1 expression and secretion in fibroblasts. Meanwhile, C1P and LPA only exhibited a minor effect on the expression and secretion of these cytokines and chemokines. Moreover, neutralization of both enzymes with anti-rLlPLD1 antibodies completely inhibited the secretion of these cytokines and chemokines. Importantly, conditioned media from fibroblasts, treated with rLlPLDs, stimulated the transmigration of THP-1 monocytes. Our data demonstrate the direct role of PLDs in chemotactic mediator synthesis for monocytes in human skin fibroblasts and indicate that inflammatory processes play an important role during loxoscelism.

  13. Acetobacter sicerae sp. nov., isolated from cider and kefir, and identification of species of the genus Acetobacter by dnaK, groEL and rpoB sequence analysis.

    Science.gov (United States)

    Li, Leilei; Wieme, Anneleen; Spitaels, Freek; Balzarini, Tom; Nunes, Olga C; Manaia, Célia M; Van Landschoot, Anita; De Vuyst, Luc; Cleenwerck, Ilse; Vandamme, Peter

    2014-07-01

    Five acetic acid bacteria isolates, awK9_3, awK9_4 ( = LMG 27543), awK9_5 ( = LMG 28092), awK9_6 and awK9_9, obtained during a study of micro-organisms present in traditionally produced kefir, were grouped on the basis of their MALDI-TOF MS profile with LMG 1530 and LMG 1531(T), two strains currently classified as members of the genus Acetobacter. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences as well as on concatenated partial sequences of the housekeeping genes dnaK, groEL and rpoB indicated that these isolates were representatives of a single novel species together with LMG 1530 and LMG 1531(T) in the genus Acetobacter, with Acetobacter aceti, Acetobacter nitrogenifigens, Acetobacter oeni and Acetobacter estunensis as nearest phylogenetic neighbours. Pairwise similarity of 16S rRNA gene sequences between LMG 1531(T) and the type strains of the above-mentioned species were 99.7%, 99.1%, 98.4% and 98.2%, respectively. DNA-DNA hybridizations confirmed that status, while amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) data indicated that LMG 1531(T), LMG 1530, LMG 27543 and LMG 28092 represent at least two different strains of the novel species. The major fatty acid of LMG 1531(T) and LMG 27543 was C18 : 1ω7c. The major ubiquinone present was Q-9 and the DNA G+C contents of LMG 1531(T) and LMG 27543 were 58.3 and 56.7 mol%, respectively. The strains were able to grow on D-fructose and D-sorbitol as a single carbon source. They were also able to grow on yeast extract with 30% D-glucose and on standard medium with pH 3.6 or containing 1% NaCl. They had a weak ability to produce acid from d-arabinose. These features enabled their differentiation from their nearest phylogenetic neighbours. The name Acetobacter sicerae sp. nov. is proposed with LMG 1531(T) ( = NCIMB 8941(T)) as the type strain. © 2014 IUMS.

  14. Simulation of Effect of Irrigation with Reclaimed Water on Soil Water-Salt Movement by ENVIRO-GRO Model%模型模拟再生水灌溉对土壤水盐运动的影响

    Institute of Scientific and Technical Information of China (English)

    吕斯丹; 陈卫平; 王美娥

    2012-01-01

    As the conflict between water supply and demand,wastewater reuse has become an important measure,which can relieve the water shortage in Beijing.In order to promote safe irrigation with reclaimed water and prevent soil salinisation,the dynamic transport of salts in urban soils of Beijing,a city of water shortage,under irrigation of reclaimed water was simulated by ENVIRO-GRO model in this research.The accumulation trends of soil salinity were predicted.Simultaneously,it investigated the effects of different irrigation practices on soil water-salt movement and salt accumulation.Results indicated that annual averages of soil salinity(ECe) increased 29.5%,97.2%,197.8% respectively,with the higher irrigation,normal irrigation,and low irrigation under equilibrium conditions.Irrigation frequency had little effect on soil salt-water movement,and soil salt accumulation was in a downward trend with low frequency of irrigation.Under equilibrium conditions,annual averages of ECe increased 23.7%,97.2%,208.5% respectively,with irrigation water salinity(ECw) 0.6,1.2,2.4 dS·m-1.Soil salinity increased slightly with ECw=0.6 dS·m-1,while soil salinization did not appear.Totally,the growth of Blue grass was not influenced by soil salinity under equilibrium conditions with the regular irrigation in Beijing,but mild soil salinization appeared.%随着水资源供需矛盾日益尖锐,污水再生利用成为了缓解北京水资源紧缺的重要举措.为推动北京再生水安全灌溉,本研究运用ENVIRO-GRO模型模拟缺水城市北京再生水灌溉土壤水盐运动规律,并对土壤盐分累积进行预测,探讨了不同灌溉措施对土壤水盐运动规律和盐分累积的影响.结果表明,高水量、常规、低水量灌溉,平衡时土壤含盐量(ECe)年均值分别增加了29.5%、97.2%、197.8%;灌溉频率对土壤水盐运动规律影响不大,但低频灌溉下土壤中盐分的累积有减少的趋势;灌溉水含盐量(ECw)为0.6、1.2、2.4 d

  15. Perspectiva de género para el análisis de la participación femenina en organizaciones ambientalistas. El caso de la organización de mujeres ecologistas de la Sierra de Petatlán, Gro

    Directory of Open Access Journals (Sweden)

    Josu00E9 Maru00EDa Aranda Su00E1nchez

    2007-01-01

    Full Text Available Con base en el análisis empírico de la Organización de Mujeres Ecologistas de la Sierra de Petatlán, Gro., este artículo propone un esquema de análisis de la participación de mujeres en organizaciones no gubernamentales ambientalistas, desde una perspectiva de género, haciendo énfasis en el estudio de las relaciones más significativas, no siempre visibles, que las mujeres establecen como activistas realizando determinadas acciones colectivas a favor del ambiente y la ecología. Esas relaciones se expresan como: a Trabajo extradoméstico; b Situación social de las mujeres; c Relaciones de las mujeres con la naturaleza, y d Relaciones de las mujeres con las instituciones.

  16. Co-overexpression of bacterial GroESL chaperonins partly overcomes non-productive folding and tetramer assembly of E. coli-expressed human medium-chain acyl-CoA dehydrogenase (MCAD) carrying the prevalent disease-causing K304E mutation

    DEFF Research Database (Denmark)

    Bross, P; Andresen, B S; Winter, V;

    1993-01-01

    underlying MCAD deficiency caused by the prevalent K304E mutation. Depending on which of the three amino acids--lysine (wild-type), glutamic acid (K304E) or glutamine (K304Q) are present at position 304 of the mature polypeptide, three different patterns were observed in our assay system: (i) solubility...... and the enzyme activity measured as observed for the wild-type protein. (iii) Solubility of the K304E mutant is in a similar fashion GroESL responsive as the K304Q mutant, but the amount of tetramer observed and the enzyme activity measured do not correlate with the amount of soluble K304E MCAD protein detected...... in Western blotting. In a first attempt to estimate the specific activity, we show that tetrameric K304E and K304Q mutant MCAD display a specific activity in the range of the wild-type enzyme. Taken together, our results strongly suggest, that the K304E mutation primarily impairs the rate of folding...

  17. GroFi: Large-scale fiber placement research facility

    Directory of Open Access Journals (Sweden)

    Christian Krombholz

    2016-03-01

    and processes for large-scale composite components. Due to the use of coordinated and simultaneously working layup units a high exibility of the research platform is achieved. This allows the investigation of new materials, technologies and processes on both, small coupons, but also large components such as wing covers or fuselage skins.

  18. GRO J0422+32 = GRS 0417+335

    DEFF Research Database (Denmark)

    Castro-Tirado, A. J.; Brandt, Søren; Lund, Niels

    1992-01-01

    , and three times brighter than, the black-hole candidate Cygnus X-1 as observed by OSSE in 1991 June. Significant emission is observed up to about 600 keV, with an average source flux of 1.8 photons cmE-2 sE-1 MeVE-1 at 100 keV. The continuum spectrum between 60 and 600 keV is not compatible with a single...

  19. Identifying Protein Stabilizing Ligands Using GroEL

    OpenAIRE

    Naik, Subhashchandra; Haque, Inamul; Degner, Nick; Kornilayev, Boris; Bomhoff, Gregory; Hodges,Jacob; Khorassani, Ara-Azad; Katayama, Hiroo; Morris, Jill; Kelly, Jeffery; Seed, John; Fisher, Mark T.

    2010-01-01

    Over the past five years, it has become increasingly apparent to researchers that the initial promise and excitement of using gene replacement therapies to ameliorate folding diseases are still far from being broadly or easily applicable. Because a large number of human diseases are protein folding diseases (~30 to 50%), many researchers now realize that more directed approaches to target and reverse the fundamental misfolding reactions preceding disease are highly feasible and offer the pote...

  20. Identifying Protein Stabilizing Ligands Using GroEL

    Science.gov (United States)

    Naik, Subhashchandra; Haque, Inamul; Degner, Nick; Kornilayev, Boris; Bomhoff, Gregory; Hodges, Jacob; Khorassani, Ara-Azad; Katayama, Hiroo; Morris, Jill; Kelly, Jeffery; Seed, John; Fisher, Mark T.

    2010-01-01

    Over the past five years, it has become increasingly apparent to researchers that the initial promise and excitement of using gene replacement therapies to ameliorate folding diseases are still far from being broadly or easily applicable. Because a large number of human diseases are protein folding diseases (~30 to 50%), many researchers now realize that more directed approaches to target and reverse the fundamental misfolding reactions preceding disease are highly feasible and offer the potential of developing more targeted drug therapies. This is also true with a large number of so called “orphan protein folding diseases”. The development of a broad-based general screening array method using the chaperonin as a detection platform will enable us to screen large chemical combinatorial libraries for specific ligands against the elusive transient, primary reactions that often lead to protein misfolding. This development will provide a highly desirable tool for the pharmaceutical, academic and medical professions. PMID:19802819

  1. GroPBS: Fast Solver for Implicit Electrostatics of Biomolecules

    Directory of Open Access Journals (Sweden)

    Franziska eBertelshofer

    2015-11-01

    Full Text Available Knowledge about the electrostatic potential on the surface of biomolecules or biomembranes under physiological conditions is an important step in the attempt to characterize the physico-chemical properties of these molecules and in particular also their interactions with each other. Additionally, knowledge about solution electrostatics may guide also the design of molecules with specified properties. However, explicit water models come at a high computational cost, rendering them unsuitable for large design studies or for docking purposes. Implicit models with the water phase treated as a continuum require the numerical solution of the Poisson-Boltzmann Equation (PBE. Here, we present a new flexible program for the numerical solution of the PBE, allowing for different geometries, and the explicit and implicit inclusion of membranes. It involves a discretization of space and the computation of the molecular surface. The PBE is solved using finite differences, the resulting set of equations is solved using a Gauss-Seidel method. It is shown for the example of the sucrose transporter ScrY that the implicit inclusion of a surrounding membrane has a strong effect also on the electrostatics within the pore region and thus need to be carefully considered e.g. in design studies on membrane proteins.

  2. Very High Energy Gamma Ray Extension of GRO Observations

    Science.gov (United States)

    Weekes, Trevor C.

    1994-01-01

    The membership, progress, and invited talks, publications, and proceedings made by the Whipple Gamma Ray Collaboration is reported for june 1990 through May 1994. Progress was made in the following areas: the May 1994 Markarian Flare at Whipple and EGRET (Energetic Gamma Ray Experiment Telescope) energies; AGN's (Active Galactic Nuclei); bursts; supernova remnants; and simulations and energy spectra.

  3. Visualisierung großer Datenmengen im Raum

    OpenAIRE

    Polowinski, Jan

    2013-01-01

    Large, strongly connected amounts of data, as collected in knowledge bases or those occurring when describing software, are often read slowly and with difficulty by humans when they are represented as spreadsheets or text. Graphical representations can help people to understand facts more intuitively and offer a quick overview. The electronic representation offers means that are beyond the possibilities of print such as unlimited zoom and hyperlinks. This paper addresses a framework for vi...

  4. GroPBS: Fast Solver for Implicit Electrostatics of Biomolecules.

    Science.gov (United States)

    Bertelshofer, Franziska; Sun, Liping; Greiner, Günther; Böckmann, Rainer A

    2015-01-01

    Knowledge about the electrostatic potential on the surface of biomolecules or biomembranes under physiological conditions is an important step in the attempt to characterize the physico-chemical properties of these molecules and, in particular, also their interactions with each other. Additionally, knowledge about solution electrostatics may also guide the design of molecules with specified properties. However, explicit water models come at a high computational cost, rendering them unsuitable for large design studies or for docking purposes. Implicit models with the water phase treated as a continuum require the numerical solution of the Poisson-Boltzmann equation (PBE). Here, we present a new flexible program for the numerical solution of the PBE, allowing for different geometries, and the explicit and implicit inclusion of membranes. It involves a discretization of space and the computation of the molecular surface. The PBE is solved using finite differences, the resulting set of equations is solved using a Gauss-Seidel method. It is shown for the example of the sucrose transporter ScrY that the implicit inclusion of a surrounding membrane has a strong effect also on the electrostatics within the pore region and, thus, needs to be carefully considered, e.g., in design studies on membrane proteins.

  5. GRO J0422+32 = GRS 0417+335

    DEFF Research Database (Denmark)

    Castro-Tirado, A. J.; Brandt, Søren; Lund, Niels;

    1992-01-01

    A. J. Castro-Tirado, S. Brandt, and N. Lund, Granat WATCH team (Danish Space Research Institute, Lyngby; and Space Research Institute, Moscow), report: "The hard x-ray transient reported by Paciesas et al. on IAUC 5580 has been observed by the WATCH all-sky monitor on Granat. The source intensity...

  6. 模型模拟土壤性质和植被种类对再生水灌溉水盐运移的影响%Simulation of Effects of Soil Properties and Plants on Soil Water-salt Movement with Reclaimed Water Irrigation by ENVIRO-GRO Model

    Institute of Scientific and Technical Information of China (English)

    吕斯丹; 陈卫平; 王美娥

    2012-01-01

    为推动再生水安全灌溉,本研究运用ENVIRO-GRO模型模拟了不同土壤性质、植被条件下土壤盐分的分布规律及累积趋势,探讨了土壤性质和植被种类对再生水灌溉水盐运移的影响.研究发现,不同土壤初始含盐量下,经多年重复模拟后最终达到统一的平衡状态;而不同土壤质地下,随着灌溉年份的增加,壤土、黏壤土中的土壤ECe年均值逐渐增加直到平衡,砂壤土中的土壤ECe年均值逐渐减少直到平衡,平衡时土层中的ECe值砂壤土〈壤土〈黏壤土.3种植被下,大叶黄杨、油松的土壤ECe值增加量小于早熟禾;土壤盐分在土层中的空间、时间分布也均有所不同.此外,不同模拟情景下,土壤盐分累积都不会影响植被的生长(黏壤土除外),但均出现轻度盐渍化(砂壤土除外).%In order to promote safe irrigation with reclaimed water and prevent soil salinisation,the dynamic transport of salts in urban soils of Beijing under irrigation of reclaimed water was simulated by ENVIRO-GRO model in this study.The accumulation trends and profile distribution of soil salinity were predicted.Simultaneously,the effects of different soil properties and plants on soil water-salt movement and salt accumulation were investigated.Results indicated that soil salinity in the profiles reached uniform equilibrium conditions by repeated simulation,with different initial soil salinity.Under the conditions of loam and clay loam soil,salinity in the profiles increased over time until reaching equilibrium conditions,while under the condition of sandy loam soil,salinity in the profiles decreased over time until reaching equilibrium conditions.The saturated soil salinity(ECe) under equilibrium conditions followed an order of sandy loamloamclay loam.Salt accumulations in Japan euonymus and Chinese pine were less than that in Blue grass.The temporal and spatial distributions of soil salinity were also different in these three

  7. Große statistische Unsicherheit beim Anteil der Top-Vermögenden in Deutschland

    OpenAIRE

    Westermeier, Christian; Grabka, Markus M.

    2015-01-01

    Analysen zur Vermögensungleichheit auf Basis von Bevölkerungserhebungen untererfassen tendenziell die Top-Vermögenden. Gerade dieser Personenkreis ist aber von besonderer Bedeutung, weil er einen beträchtlichen Teil des Gesamtvermögens besitzt. Offizielle Registerdaten zur Vermögenssituation liegen für Deutschland nicht vor, die Top-Vermögen lassen sich lediglich an Hand von "Reichen-Listen" simulieren. Kombiniert man etwa die Forbes-Liste, die rund 50 Dollar-Milliardäre mit deutscher Staatsb...

  8. La arquitectura de la Groβstadt : Berlín, 1900-1945

    Directory of Open Access Journals (Sweden)

    Mónica Vázquez Astorga

    2011-01-01

    Full Text Available El presente artículo recoge las conclusiones más importantes obtenidas durante mi estancia de investigación realizada en Berlín en el verano de 2009, que tuvo por objeto el estudio y análisis de la actividad arquitectónica desarrollada en Berlín antes y después de la Segunda Guerra Mundial y, en concreto, entre 1900 y 1945. Se valora también el contexto existente en esta ciudad tras las destrucciones ocasionadas por el segundo conflicto mundial, aludiendo a las diversas propuestas ideadas para su reconstrucción. Asimismo, se ha buscado establecer paralelismos con el panorama arquitectónico europeo.The following article includes the most important conclusions drawn from my research stay in Berlin during the summer of 2009. The main aim of my research was to study and analyse the architectural development carried out in Berlin between 1900 and 1945, before and after the II World War, taking also into account the prevailing context of the city due to the damages caused by this War, the numerous reconstruction projects put forward in that moment, together with an attempt to establish parallelisms with the European architectural prospect of that historical moment.

  9. Wissenschaftler wollen mit dem Großen Hadronenbeschleuniger bei Genf Welträtsel knacken

    CERN Multimedia

    2008-01-01

    The way to the Big Bang is 27 kilometers long. The ring of the new particle accelerator of CERN has this extent; from May protons - which is positively charged parts of the atomic nucleus - will start collide almost with speed of light hit one on the other.

  10. 76 FR 39812 - Scotts Miracle-Gro Co.; Regulatory Status of Kentucky Bluegrass Genetically Engineered for...

    Science.gov (United States)

    2011-07-07

    ... Internet at http://www.aphis.usda.gov/biotechnology/news.shtml . FOR FURTHER INFORMATION CONTACT: Dr. Andrea Huberty, Branch Chief, Regulatory and Environmental Analysis Branch, Biotechnology Regulatory... restriction is necessary to prevent the introduction of a plant pest or noxious weed into the United States or...

  11. Relativität, Quanten theorie und Große Vereinigung

    CERN Multimedia

    2005-01-01

    Albert Einstein's general relativity theory is so successful - It remains a classic description, that considers no quantum effects. In t his interview, Hermann Nicolai, Director of the Max Planck Institute for gravitationnal physics in Postdam, describe possible ways to come to a standardized theory

  12. The large Nuremberg clock (German Title: Die große Nürnbergische Uhr)

    Science.gov (United States)

    Gaab, Hans

    The great Nuremberg Clock denotes a specialty in the way of counting hours in the city of Nuremberg, which was in use for a long time. Hours of equal length, as they are also indicated by wheel clocks, were used, the partition into daily and nightly hours was kept, however, the number of these (daily and nightly) hours must be an integer value. Accordingly, the length of the day had to be increased or shortened by an hour in regular intervals. Tables were calculated for this purpose, which also listed the ringing times of church bells.

  13. Eval dome Makur uation estic an rdi met n of gro nd irrig tropoli ...

    African Journals Online (AJOL)

    sunny

    dwater abstracted through hand-dug wells and boreholes as their source of ... and environs and evaluate its suitability for drinking, domestic and ... reous and non-calcareous shale, sandy or shally lime- stone and .... 2) showing the relationship between the measured parameters. ..... HCO3 in order of increasing dominance.

  14. Gutshof Dagö-Großenhof/Hiiu-Suuremõisa mõis / Arne von Maydell

    Index Scriptorium Estoniae

    Maydell, Arne von

    2014-01-01

    Ühest parimast säilinud hilisbaroki näitest Eestis - Hiiu-Suuremõisast. Mõisapark on suurim Hiiumaal, 17. sajandi algusest kuni 19. sajandini oli mõis üks Hiiumaa majanduskeskustest. Mõisa omanikest. Härrastemajaga seotud müstikast

  15. Die Suche nach dem verschollenen Großsteingrab G4 'Onner es' (Onnen, Prov. Groningen)

    NARCIS (Netherlands)

    Groenendijk, Hendrik; Lanting, JN; Woldring, H

    2014-01-01

    On the eastern hillside of the Hondsrug near the hamlet Onnen (Gr.), a scatter of surface finds led to the belief that a megalithic grave (hunebed) must have been present on the Onner es. Through soil radar, test excavation, pollen analysis and coring, the former location of the presumed hunebed was

  16. A Research of 140-GHz Folded Rectangular Gro ove Waveguide Traveling-Wave Tub e

    Institute of Scientific and Technical Information of China (English)

    ZHANG Minghao; WEI Yanyu; YUE Lingna; GUO Guo; WANG Yuanyuan; SHI Xianbao; WANG Wenxiang

    2015-01-01

    A two-section Folded rectangular groove waveguide (FRGWG) Slow wave structure (SWS) Travel-ing wave tube (TWT) with large dimension of beam tunnel is studied. Compared with the Folded waveguide (FWG) under the same size parameters conditions, the interac-tion impedance and center frequency of the FRGWG are higher. The advantage is that a beam tunnel with large dimension can be applied to the FRGWG without the influence caused by signal decrease, reflection and oscil-lation. The microwave amplification capability based on beam-wave interaction is obtained through the particle-in-cell method. This circuit structure can produce an output power of over 100W ranging from 136 to 142GHz when the operation voltage and beam current are set as 18.4kV and 150mA, respectively, for a 95mm long circuit.

  17. Bodensee-Oberschwaben. Eine erfolgreiche Region fernab der Großstädte

    Science.gov (United States)

    Danielzyk, Rainer; Köhler, Stefan; Friedsmann, Philipp

    2017-09-01

    Bodensee-Oberschwaben is representative of a type of region that does not receive much attention in regional research. The region is located peripherally, away from the metropolises and urban agglomeration areas. Despite seemingly poor initial conditions, the region is economically very successful. A polycentric structure of many small and medium-sized towns characterize Bodensee-Oberschwaben as well as other attributes, which do not allow for classification into traditional categories such as rural or urban. Friedrichshafen, the largest city and the industrial core of the region, contributes significantly to its prosperity. The international high technology companies, which are located here, have their origins in the activities of Graf Zeppelin in the early 20th century. Regional universities and the strong network between actors both within the region as well as between Bodensee-Oberschwaben and the neighboring regions have also had a positive impact on region's development.

  18. OMC camera experiment for INTEGRAL and search for Compton GRO BATSE LOCBURST optical transients

    Science.gov (United States)

    Rezek, Tomáš; Hudec, René; Hroch, Filip; Soldán, Jan; Mas-Hesse, Miguel; Giménez, Alvaro

    1998-05-01

    The test camera of the Optical Monitoring Camera (OMC) experiment for INTEGRAL spacecraft achieving an angular pixel size of 18 arcsec and a field of view 7.5°×5.1° has been successfully developed and tested at the Astronomical Institute Ondřejov. The test camera is able to provide imaging down to 15 mag over the whole field of view within one exposure of 300 seconds. Although developed primarily to test the OMC performance and help with software development, this device is ideally suited for use as a ground-based camera for sites where Compton Gamma Ray Observatory BATSE Locburst triggers are followed-up in the optical waveband and also for wide-field sky monitoring in general. The low cost of this camera makes it possible to duplicate the system at a number of observing sites. A chart and a corresponding CCD-image for the BACODINE Locburst Position 6368 taken with the OMC test camera at Ondřejov observatory are also presented. The image taken 18 hours after the trigger was computer-blinked with the frame taken 30 days later. No optical activity has been found down to 13.5 mag.

  19. Das Augustusnomen Karls des Großen und das karolingische Imperium

    OpenAIRE

    Strothmann, Jürgen

    2015-01-01

    The Nomen Augusti of Charlemagne and the Carolingian imperium. –The Nomen Augusti of Charlemagne is on a first view taken from the byzantine custom and in this respect is a pure title for an emperor. But for the Carolingians and Frankish elites it does mean more than this. The regular title for an emperor there is caesar or imperator, but augustus evokes remembering Octavian-Augustus himself too. That is shown by the Carolingian poets. The key for this conviction lies in the selfconsciousness...

  20. Authormagic: Ein Konzept zur Autorenidentifikation in Großen Digitalen Bibliotheken

    CERN Document Server

    Weiler, Henning

    2012-01-01

    Author name ambiguities distort the quality of information discovery in digital libraries. These ambiguities also contribute to the inaccurate attribution of authorship to individual researchers. The latter is especially delicate in research evaluation. To solve this issue, many algorithmic bulk disambiguation approaches have been proposed in the literature. However, no algorithmic approach can solve author ambiguities with an accuracy of 100%. Some online projects allow users to manually create publication lists, which are then regarded as profiles of the researchers. The tedious work to manually assemble such publication lists and the unavailability of scientific material in these projects limit the success of these projects. The “Authormagic” concept is developed in this thesis to address the author ambiguity issue with a hybrid approach of combining algorithmic and human intelligence. A customized agglomerative clustering approach first determines publication clusters by comparing available metadata. ...

  1. Confirmation of a high magnetic field in GRO J1008-57

    DEFF Research Database (Denmark)

    Bellm, Eric C.; Fuerst, Felix; Pottschmidt, Katja

    2014-01-01

    search to date for cyclotron scattering features at lower energies. We find evidence for a 78(-2)(+3) keV line in the NuSTAR and Suzaku data at >4 sigma significance, confirming the detection using Suzaku alone by Yamamoto et al. A search of both the phase-averaged and phase-resolved data rules out...

  2. Fourteen Years of Glory——An Interview With NPC Vice-Chairman Radi

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    Editor's Note: Radi, a Tibetan, was born in August 1938 in Biru County, Tibet. He is a vice-chairman of the NPC Standing Committee.A serf in old Tibet, he was a Tibetan leader from 1975 to 2003. We interviewed him at the end of 2003,and he talked about changes that have taken place in Tibet in the past 14 years.

  3. A Study of Postmodern Narrative in Akbar Radi's Khanomche and Mahtabi

    Directory of Open Access Journals (Sweden)

    Hajar Abbasi Narinabad

    2012-09-01

    Full Text Available This project aims to explore representation of narratology based on some recent postmodern theories backed up by the ideas of some postmodern discourses including Roland Barthes, Jean-François Lyotard, Julia Kristeva and William James with an especial focus on Akbar Radi-the Persian Playwright- drama Khanomche and Mahtabi .The article begins by providing a terminological view of narrative in general and postmodern narrative in specific. As postmodern narrative elements are too general and the researcher could not cover them all, she has gone through the most eminent elements: intertextuality, stream of consciousness style, fragmentation and representation respectively which are delicately utilized in Khanomche and Mahtabi. The review then critically applies the theories on the mentioned drama. The article concludes by recommending a few directions for the further research.

  4. Rapid, Sensitive Detection of Bartonella quintana by Loop-Mediated Isothermal Amplification of the groEL Gene

    Science.gov (United States)

    Hu, Shoukui; Niu, Lina; Luo, Lijuan; Song, Xiuping; Sun, Jimin; Liu, Qiyong

    2016-01-01

    Trench fever, caused by Bartonella quintana, is recognized as a re-emerging and neglected disease. Rapid and sensitive detection approaches are urgently required to monitor and help control B. quintana infections. Here, loop-mediated isothermal amplification (LAMP), which amplifies target DNA at a fixed temperature with high sensitivity, specificity and rapidity, was employed to detect B. quintana. Thirty-six strains, including 10 B. quintana, 13 other Bartonella spp., and 13 other common pathogens, were applied to verify and evaluate the LAMP assay. The specificity of the LAMP assay was 100%, and the limit of detection was 125 fg/reaction. The LAMP assay was compared with qPCR in the examination of 100 rhesus and 20 rhesus-feeder blood samples; the diagnostic accuracy was found to be 100% when LAMP was compared to qPCR, but the LAMP assay was significantly more sensitive (p < 0.05). Thus, LAMP methodology is a useful for diagnosis of trench fever in humans and primates, especially in low-resource settings, because of its rapid, sensitive detection that does not require sophisticated equipment. PMID:27916953

  5. Rapid, Sensitive Detection of Bartonella quintana by Loop-Mediated Isothermal Amplification of the groEL Gene

    Directory of Open Access Journals (Sweden)

    Shoukui Hu

    2016-12-01

    Full Text Available Trench fever, caused by Bartonella quintana, is recognized as a re-emerging and neglected disease. Rapid and sensitive detection approaches are urgently required to monitor and help control B. quintana infections. Here, loop-mediated isothermal amplification (LAMP, which amplifies target DNA at a fixed temperature with high sensitivity, specificity and rapidity, was employed to detect B. quintana. Thirty-six strains, including 10 B. quintana, 13 other Bartonella spp., and 13 other common pathogens, were applied to verify and evaluate the LAMP assay. The specificity of the LAMP assay was 100%, and the limit of detection was 125 fg/reaction. The LAMP assay was compared with qPCR in the examination of 100 rhesus and 20 rhesus-feeder blood samples; the diagnostic accuracy was found to be 100% when LAMP was compared to qPCR, but the LAMP assay was significantly more sensitive (p < 0.05. Thus, LAMP methodology is a useful for diagnosis of trench fever in humans and primates, especially in low-resource settings, because of its rapid, sensitive detection that does not require sophisticated equipment.

  6. "Groβes zärtliches Brasilien": Das Brasilienbild in den Werken von Heinrich Eduard Jacob

    Directory of Open Access Journals (Sweden)

    Marlen Eckl

    2009-12-01

    Full Text Available Embora tendo viajado pelo Brasil em 1932 e depois escrito vários artigos sobre essa viagem assim como três livros de temática brasileira, Heinrich Eduard Jacob é quase desconhecido em Brasil. Por isso neste artigo são apresentados a trajetória do jornalista e escritor e as suas obras brasileiras Treibhaus Südamerika (1934, Saga e marcha triunfal do café (1934 e Estrangeiro. Einwandererschicksal in Brasilien (1951. Ademais Jacob será contextualizado atraves da conecção com os outros autores que, nos anos 30 e 40, escreveram sobre o país. Estes, na maioria, foram refugiados do nazismo como entre outros Stefan Zweig, Richard Katz, Wolfgang Hoffmann-Harnisch, Frank Arnau e Marte Brill. Quanto ao imaginário acerca do Brasil descrito por Jacob percebe-se que ele como tantos outros autores também viu no Brasil um "país de um futuro" (como o chamou e uma "democracia racial" pretensa existente no país. Apesar disso, não desistiu de desmascarar a cordialidade brasileira proverbial como sendo apenas disfarce e de indicar problemas existentes como a questão da política quanto ao café e as consequencias negativas do nacionalismo, fomentado pelo regime Vargas. Assim ele defendeu um ponto de vista que foi contrário àquele do seu amigo Stefan Zweig.

  7. The rule-based language XL and the modelling environment GroIMP illustrated with simulated tree competition

    NARCIS (Netherlands)

    Hemmerling, R.; Kniemeyer, O.; Lanwert, D.; Kurth, W.; Buck-Sorlin, G.H.

    2008-01-01

    The programming language XL ('eXtended L-system language') is an extension of Java, which supports the specification and execution of relational growth grammars, a variant of parallel graph grammars. XL is a powerful generalisation of the well-known L-system approach to functional-structural plant m

  8. Basisindikatoren der ökonomischen Entwicklung in Deutschland, Großbritannien, Schweden, USA - 1950 bis 2000

    OpenAIRE

    Thome, Helmut; Birkel, Christoph

    2005-01-01

    Die Vergleichsstudie präsentiert Daten zur ökonomischen Entwicklung in Deutschland, England, Schweden und den USA im Zeitraum 1950-2000. Dabei gliedern sich die Ausführungen in folgende ökonomische Kenngrößen: (1) Wachstum und Niveau des Volkseinkommens, (2) Produktivitätsentwicklung, (3) Arbeitslosigkeit, (4) Beschäftigungsstrukturen (Arbeitszeitvolumen und Beschäftigungsquoten, sektorale Entwicklung, öffentlicher und privater Sektor, Frauenerwerbsquote, Selbständigenquote) (5) Entwicklung d...

  9. GRO/EGRET data analysis software: An integrated system of custom and commercial software using standard interfaces

    Science.gov (United States)

    Laubenthal, N. A.; Bertsch, D.; Lal, N.; Etienne, A.; Mcdonald, L.; Mattox, J.; Sreekumar, P.; Nolan, P.; Fierro, J.

    1992-01-01

    The Energetic Gamma Ray Telescope Experiment (EGRET) on the Compton Gamma Ray Observatory has been in orbit for more than a year and is being used to map the full sky for gamma rays in a wide energy range from 30 to 20,000 MeV. Already these measurements have resulted in a wide range of exciting new information on quasars, pulsars, galactic sources, and diffuse gamma ray emission. The central part of the analysis is done with sky maps that typically cover an 80 x 80 degree section of the sky for an exposure time of several days. Specific software developed for this program generates the counts, exposure, and intensity maps. The analysis is done on a network of UNIX based workstations and takes full advantage of a custom-built user interface called X-dialog. The maps that are generated are stored in the FITS format for a collection of energies. These, along with similar diffuse emission background maps generated from a model calculation, serve as input to a maximum likelihood program that produces maps of likelihood with optional contours that are used to evaluate regions for sources. Likelihood also evaluates the background corrected intensity at each location for each energy interval from which spectra can be generated. Being in a standard FITS format permits all of the maps to be easily accessed by the full complement of tools available in several commercial astronomical analysis systems. In the EGRET case, IDL is used to produce graphics plots in two and three dimensions and to quickly implement any special evaluation that might be desired. Other custom-built software, such as the spectral and pulsar analyses, take advantage of the XView toolkit for display and Postscript output for the color hard copy. This poster paper outlines the data flow and provides examples of the user interfaces and output products. It stresses the advantages that are derived from the integration of the specific instrument-unique software and powerful commercial tools for graphics and statistical evaluation. This approach has several proven advantages including flexibility, a minimum of development effort, ease of use, and portability.

  10. Época de recolecta en Palo Morado (Peltogyne mexicana M., en Acapulco Gro., México

    Directory of Open Access Journals (Sweden)

    Alejandro Juárez-Agis

    2013-02-01

    Full Text Available En seis arboles de Peltogyne mexicana M. se evaluó el efecto de las fechas sobre variables de germinación, Se utilizó un diseño de bloques al azar con parcelas divididas con cinco bloques (repeticiones, en las parcelas grandes se colocaron los árboles (cinco y en las parcelas chicas las fechas de recolecta (seis. Cada parcela pequeña tuvo cinco bolsas, estas a su vez tuvieron cinco semillas, usándose 25 semillas de cada fecha de su respectivo árbol (parcela pequeña en cada parcela grande (fecha de recolecta. Los mejores resultados para germinación fueron para la fecha uno con un valor de 72.48% y la fecha dos con valor de 61.12%, el valor mínimo fue de 24.48 para la fecha seis. Así las semillas que se recolectan de manera temprana tienen porcentajes de germinación mayor

  11. Constraints of age, distance and progenitor of the supernova remnant RX J0852-4622/GRO J0852-4642

    OpenAIRE

    Aschenbach, Bernd; Iyudin, Anatoli F.; Schoenfelder, Volker

    1999-01-01

    The discovery of the nearest young supernova remnant RXJ0852-4622/GROJ0852-4642 in the Galaxy by ROSAT and COMPTEL has been reported recently. Age and distance are determined to ~680 years and ~200 pc by the X-ray diameter and the gamma-ray line flux of radioactive 44Ti. Here we discuss the implications of the X-ray spectra and of the fact that 1.8 MeV gamma-ray line emission from the decay of 26Al has been measured from the Vela region with a certain fraction possibly associated with the new...

  12. Klinische Ergebnisse nach stereotaktischer Präzisionsbestrahlung großer uvealer Melanome mit dem Gamma-Knife

    OpenAIRE

    Schonenbach, Sabine

    2006-01-01

    Von Juni 1997 bis April 2001 wurden 97 Patienten, die an einem unilateralen uvealen Melanom litten, mit dem Gamma-Knife radiochirurgisch behandelt. Die Melanome aller 97 Patienten waren aufgrund der Lokalisation oder der Größe für eine Therapie mit Ruthenium-Applikatoren nicht geeignet. Alle 97 Patienten, die sich der Therapie mit dem Gamma-Knife unterzogen, wurden in eine engmaschige Nachsorge aufgenommen. Bei der Nachsorge wurden in regelmäßigen Abständen klinische, echographische und n...

  13. Le CERN et l'embargo des Nations Unies envers la Serbie et le Monténégro

    CERN Document Server

    CERN Press Office. Geneva

    1993-01-01

    CERN, the European Laboratory of Particle Physics, in reply to questions raised by the media would like to clarify its position with regard to the resolution No 757 of the U.N. Security Council of 30 May 1992 imposing an embargo on the Federal Republic of Yugoslavia (Serbia and Montenegro). This position was adopted immediately after the embargo was announced.

  14. Die Messung der Gluonpolarisation durch die Produktion von Hadronpaaren mit großen Transversalimpulsen in tiefinelastischer Myonstreuung am Nukleon

    CERN Document Server

    Hermann, Roman

    2009-01-01

    The spin structure of the nucleon is investigated at the COMPASS experiment at the CERN-SPS using polarized muons scattering off polarized nucleons. The contribution of the quarks to nucleon spin, as measured in the inclusive deep-inelastic scattering, is not sufficient to explain the spin of the nucleon. Thus it has to be clarified how the gluon polarization and the angular momenta of quarks and gluons contribute to the spin of the nucleon. Since the gluon polarization can only be estimated from the $Q^{2}$-dependence of inclusive deep inelastic asymmetries, a direct measurement of the gluon polarization is mandatory. The COMPASS collaboration determines the gluon polarization from cross section asymmetries in photon-gluon fusion processes using open charm production or the production of hadron pairs with large transverse momenta. This thesis presents a measurement of the gluon polarization using the COMPASS data of the years 2003 and 2004. The events with large virtuality, $Q^{2}>1$ $GeV^{2}/c^{2}$ and hadr...

  15. Untersuchungen zur Populationsdynamik von Flöhen auf Hunden und Katzen im Großraum Regensburg

    OpenAIRE

    Biebel, Sandra

    2007-01-01

    Investigations on the Population Dynamics of Fleas in Dogs and Cats in the Region of Regensburg, Germany The objective of this study was to compile epidemiological data about the occurrence and distribution of flea infestation by parasitological screening of dogs and cats in the area of Regensburg. Simultaneously, pet owners have been interviewed by use of a questionnaire regarding their experience with flea infestation of their animals and in the environment. The mean infestation rate...

  16. „Vater einer großen Nachkommenschaft von Forschungsreisenden ...“ Ehrungen Alexander von Humboldts im Jahre 1869

    Directory of Open Access Journals (Sweden)

    Ilse Jahn

    2004-04-01

    Full Text Available Article in German, Abstracts in English and GermanIn 1869 the “Naturwissenschaftlicher Verein” was founded in Magdeburg (Saxony Anhalt. That was the time when the scientific world celebrated Alexander von Humboldt’s 100th birthday. In this context, Humboldt’s achievements were remembered during the founding ceremony of that society. The paper which was read in September 2003 on a meeting of this organisation, focuses on three famous naturalists who celebrated Humboldt because they owed him much help in their own professional careers. The botanist Matthias Jacob Schleiden gave a memorial speech in 1869 in Dresden. The physiologist and pioneer of electro-biology Emil du Bois-Reymond honoured his mentor A. v. Humboldt on the occasion of the dedication of the statues of Wilhelm and Alexander in 1883 in Berlin. Charles Darwin expressed his gratitude to Humboldt by sending him in 1839 the report of his travels and received an interesting reply. These three scholars represented a new epoch in the development of the natural sciences. The paper includes a recently re-discovered letter from Humboldt to Schleiden, dated April 13, 1842.

  17. Intracellular delivery of recombinant arginine deiminase (rADI) by heparin-binding hemagglutinin adhesion peptide restores sensitivity in rADI-resistant cancer cells.

    Science.gov (United States)

    Wu, Fe-Lin Lin; Yeh, Tzyy-Harn; Chen, Ying-Luen; Chiu, Yu-Chin; Cheng, Ju-Chen; Wei, Ming-Feng; Shen, Li-Jiuan

    2014-08-04

    Recombinant arginine deiminase (rADI) has been used in clinical trials for arginine-auxotrophic cancers. However, the emergence of rADI resistance, due to the overexpression of argininosuccinate synthetase (AS), has introduced an obstacle in its clinical application. Here, we have proposed a strategy for the intracellular delivery of rADI, which depletes both extracellular and intracellular arginine, to restore the sensitivity of rADI-resistant cancer cells. In this study, the C terminus of heparin-binding hemagglutinin adhesion protein from Mycobacterium tuberculosis (HBHAc), which contains 23 amino acids, was used to deliver rADI into rADI-resistant human breast adenocarcinoma cells (MCF-7). Chemical conjugates (l- and d-HBHAc-SPDP-rADI) and a recombinant fusion protein (rHBHAc-ADI) were produced. l- and d-HBHAc-SPDP-rADI showed a significantly higher cellular uptake of rADI by MCF-7 cells compared to that of rADI alone. Cell viability was significantly decreased in a dose-dependent manner in response to l- and d-HBHAc-SPDP-rADI treatments. In addition, the ratio of intracellular concentration of citrulline to arginine in cells treated with l- and d-HBHAc-SPDP-rADI was significantly increased by 1.4- and 1.7-fold, respectively, compared with that obtained in cells treated with rADI alone (p < 0.001). Similar results were obtained with the recombinant fusion protein rHBHAc-ADI. Our study demonstrates that the increased cellular uptake of rADI by HBHAc modification can restore the sensitivity of rADI treatment in MCF-7 cells. rHBHAc-ADI may represent a novel class of antitumor enzyme with an intracellular mechanism that is independent of AS expression.

  18. RNA interference of argininosuccinate synthetase restores sensitivity to recombinant arginine deiminase (rADI in resistant cancer cells

    Directory of Open Access Journals (Sweden)

    Yo Hao-Hsin

    2011-04-01

    Full Text Available Abstract Background Sensitivity of cancer cells to recombinant arginine deiminase (rADI depends on expression of argininosuccinate synthetase (AS, a rate-limiting enzyme in synthesis of arginine from citrulline. To understand the efficiency of RNA interfering of AS in sensitizing the resistant cancer cells to rADI, the down regulation of AS transiently and permanently were performed in vitro, respectively. Methods We studied the use of down-regulation of this enzyme by RNA interference in three human cancer cell lines (A375, HeLa, and MCF-7 as a way to restore sensitivity to rADI in resistant cells. The expression of AS at levels of mRNA and protein was determined to understand the effect of RNA interference. Cell viability, cell cycle, and possible mechanism of the restore sensitivity of AS RNA interference in rADI treated cancer cells were evaluated. Results AS DNA was present in all cancer cell lines studied, however, the expression of this enzyme at the mRNA and protein level was different. In two rADI-resistant cell lines, one with endogenous AS expression (MCF-7 cells and one with induced AS expression (HeLa cells, AS small interference RNA (siRNA inhibited 37-46% of the expression of AS in MCF-7 cells. ASsiRNA did not affect cell viability in MCF-7 which may be due to the certain amount of residual AS protein. In contrast, ASsiRNA down-regulated almost all AS expression in HeLa cells and caused cell death after rADI treatment. Permanently down-regulated AS expression by short hairpin RNA (shRNA made MCF-7 cells become sensitive to rADI via the inhibition of 4E-BP1-regulated mTOR signaling pathway. Conclusions Our results demonstrate that rADI-resistance can be altered via AS RNA interference. Although transient enzyme down-regulation (siRNA did not affect cell viability in MCF-7 cells, permanent down-regulation (shRNA overcame the problem of rADI-resistance due to the more efficiency in AS silencing.

  19. RADI's Airborne X-SAR with High Resolution: Performance, Characterization and Verification

    Science.gov (United States)

    Shen, T.; Li, J.; Wang, Z. R.; Huang, L.

    2016-11-01

    X-SAR is an airborne multi-mode synthetic aperture radar (SAR) system with high- resolution, interferometer and full-polarization, developed by the Institute of Remote Sensing and Digital Earth (RADI), Chinese Academy of Sciences (CAS), funded by the CAS Large Research Infrastructures. Since 2009, the first developed stage of X-SAR system was successfully implemented to an operational SAR with high resolution (up to 0.5 meter). In May 2013, the imaging verification on flights test was carried out. The data calibration on the laboratory measurements were completed at the end of 2015. Many valuable results of imaging verification and data calibration have emphasized the quantitative microwave measurement capabilities. This paper presents the results of X-SAR system performance, characterization, optimization, and verification as carried out during the flight trials and laboratory measurement. The system performance and calibration parameters are presented such as transmitter amplitude accuracy, phase noise, system gain change with temperature variation, long-term radiometric stability. The imaging verification of the key performance parameters is discussed, including target-response function, target pairs discrimination, image noise and radiometric resolution. The example imagery of radiometric enhanced products for intensity change detection is also described.

  20. On the reducible character of Haldane-Radić enzyme kinetics to conventional and logistic Michaelis-Menten models.

    Science.gov (United States)

    Putz, Mihai V

    2011-04-13

    The conceptual and practical issues regarding the reduction of the Haldane-Radić enzymic mechanism, specific for cholinesterase kinetics, to the consecrated or logistically modified Michaelis-Menten kinetics, specific for some mutant enzymes, are here clarified as due to the limited initial substrate concentration, through detailed initial rate and progress curve analysis, even when other classical conditions for such equivalence are not entirely fulfilled.

  1. On the Reducible Character of Haldane-Radić Enzyme Kinetics to Conventional and Logistic Michaelis-Menten Models

    Directory of Open Access Journals (Sweden)

    Mihai V. Putz

    2011-04-01

    Full Text Available The conceptual and practical issues regarding the reduction of the Haldane-Radić enzymic mechanism, specific for cholinesterase kinetics, to the consecrated or logistically modified Michaelis-Menten kinetics, specific for some mutant enzymes, are here clarified as due to the limited initial substrate concentration, through detailed initial rate and progress curve analysis, even when other classical conditions for such equivalence are not entirely fulfilled.

  2. Different shapes of modernism/neoclassicism: Case study of Dušan Radić’s creative output

    Directory of Open Access Journals (Sweden)

    Mikić Vesna

    2006-01-01

    Full Text Available This paper deals with musical neoclassicism in the context of modernism, and shows that some neoclassical features, usually considered to be reactionary and retrograde, could and should be understood as modernist. This kind of contextualization is especially important when Serbian postwar music is in question. Neoclassicism in Serbian music began in a specific ideological milieu. Paradoxically, and despite its "call for order" characteristics, it turned out to be a kind of modernistic phenomenon. On one hand it received all typical shapes of European modernism, while on the other it remains as a typical idiom of postwar Serbian musical production, blending in perfectly with the postmodern. These issues are argued and proved through analysis of some pieces written by Dušan Radić during the 1950’s. We see a kind of "disguised" modernism in the composer's subversion of the norms of national romanticism and socialist realism in the neoclassical choir piece Gungulice (1953, a modernism that is based on Freud's "reaction-formation" notion. Then, a more radical kind of modernism is followed in the piece The List for chamber ensemble (1952– 1954, arguing the notion of radical in the context of Serbian music, and coming to the conclusion that different interpretations of this piece as avant-garde, radical, or neoclassical/modernist can occur as a consequence of the listener's position. In the case of The Tower of Sculls we observe the same kind of "disguised" modernism, but now directly interwoven with proto-postmodern elements, coming from popular music practices, which is also the case in other pieces by Dušan Radić. The composer’s nonconformity, lack of pretentiousness, and inclination toward the simplicity of everyday things form his modernistic identity.

  3. Radioactive contamination mapping of northeastern and eastern Japan by a car-borne survey system, Radi-Probe.

    Science.gov (United States)

    Kobayashi, Shingo; Shinomiya, Takayuki; Kitamura, Hisashi; Ishikawa, Takahiro; Imaseki, Hitoshi; Oikawa, Masakazu; Kodaira, Satoshi; Miyaushiro, Norihiro; Takashima, Yoshio; Uchihori, Yukio

    2015-01-01

    We constructed a new car-borne survey system called Radi-Probe with a portable germanium gamma-ray spectrometer onboard a cargo truck, to identify radionuclides and quantify surface contamination from the accident at Fukushima Dai-ichi Nuclear Power Station. The system can quickly survey a large area and obtain ambient dose equivalent rates and gamma-ray energy spectra with good energy resolution. We also developed a new calibration method for the system to deal with an actual nuclear disaster, and quantitative surface deposition densities of radionuclides, such as (134)Cs and (137)Cs, and kerma rates of each radionuclide can be calculated. We carried out car-borne survey over northeastern and eastern Japan (Tohoku and Kanto regions of Honshu) from 25 September through 7 October 2012. We discuss results of the distribution of ambient dose equivalent rate H(∗)(10), (134)Cs and (137)Cs surface deposition densities, spatial variation of (134)Cs/(137)Cs ratio, and the relationship between surface deposition densities of (134)Cs/(137)Cs and H(∗)(10). The ratio of (134)Cs/(137)Cs was nearly constant within our measurement precision, with average 1.06 ± 0.04 in northeastern and eastern Japan (decay-corrected to 11 March, 2011), although small variations from the average were observed.

  4. 成纤维细胞生长因子受体的结构与功能%Structure and Function of Fibroblast Gro wth Factor Receptor

    Institute of Scientific and Technical Information of China (English)

    叶展; 赵涵芳; 钱关祥

    2003-01-01

    @@ 目前已发现的成纤维细胞生长因子(fibroblast growth factor, FGF)家族至少有19个成员(FGF1-19)[1],它们在发育、血管生成、创伤愈合中有重要作用.而它们的功能是通过与成纤维细胞生长因子受体(fibroblast growth factor receptor, FGFR)结合后,经过信号传递系统,激活相应的基因而实现的.本文主要介绍FGFR结构和功能的基础知识与研究近况.

  5. Fritz Hochwälders Großstadtroman «Donnerstag» oder Wie sich Schnitzler-Szenarien mit Horváth-Figuren erden lassen

    Directory of Open Access Journals (Sweden)

    Evelyne Polt-Heinzl

    2012-06-01

    Full Text Available The 155 scenes of Fritz Hochwälder’s posthumous novel group around one central idea: each of the characters expects a final decision from the coming Thursday. What was read as a loosely connected series of expressionistic short scenes reveals itself as a carefully constructed whole. The narrative cement is provided by erotic relationships, by the visualization of metropolitan patterns of motion, and by a tightly woven texture of motives. Through emotional and economic treason, Hochwälder puts Schnitzler’s language of love in the mouths of characters from Horváth’s narrative worlds, and he exploits an up to now overlooked criminal plot.

  6. Fritz Hochwälders Großstadtroman «Donnerstag» oder Wie sich Schnitzler-Szenarien mit Horváth-Figuren erden lassen

    OpenAIRE

    Evelyne Polt-Heinzl

    2012-01-01

    The 155 scenes of Fritz Hochwälder’s posthumous novel group around one central idea: each of the characters expects a final decision from the coming Thursday. What was read as a loosely connected series of expressionistic short scenes reveals itself as a carefully constructed whole. The narrative cement is provided by erotic relationships, by the visualization of metropolitan patterns of motion, and by a tightly woven texture of motives. Through emotional and economic treason, Hochwälder puts...

  7. Effects of dietary prebiotic GroBiotic®- A on growth performance, plasma thyroid hormones and mucosal immunity of great sturgeon, Huso huso (Linnaeus, 1758)

    DEFF Research Database (Denmark)

    Adel, M.; Nayak, S.; Lazado, Carlo Cabacang

    2016-01-01

    different levels (i.e. 0.0% as control, 0.5%, 1% and 2%, in three replicates, 20 fish per replicate) and fed to the fish for an 8-week period wherein 240 fish were cultured in 1,800-L fiberglass tanks that formed part of a flow-through system. Water temperature was maintained at 20.4 ± 1.5°C. Significant...

  8. Die Regenwurmfauna (Oligochaeta: Lumbricidae der Aue der Großen Kokel als Abhängige der Böden im Flusseinzugsgebiet und der Auengradienten

    Directory of Open Access Journals (Sweden)

    Höser, N.

    2011-06-01

    Full Text Available In five floodplain transects along the course of the river Târnava Mare in Rumania, 17 earthworm species werecounted. The species spectrum of the earthworm fauna of the studied floodplains is dependent on the provenance of thefloodplain sediments. On the basis of this provenance, the fauna of three groups of soil localities on the floodplain aredifferentiated: in the upper reaches of the river, the fauna of the floodplain soils derived from brown forest soils and podzolicsoils (near Sub Cetate; on the eastern side of the middle reaches, the fauna of the soils mainly derived from gray-brown podzolicsoils (near Porumbenii Mari, Hoghilag; and on the western side of the middle reaches and the lower reaches, the fauna of thesoils principally derived from planosols (near Alma, Valea Lungă. Proctodrilus tuberculatus occurs in the floodplain sedimentspredominantly derived from the gray-brown podzolic soils, and in those parts of the floodplain derived from planosols P.opisthoductus and Aporrectodea georgii are found. Ap. georgii and Allolobophora leoni are mostly mutually exclusive in thefloodplain transect, were they are apparently indicator species of sediment deposition close to the river and/or the effect of highwater on the soil chemistry. P. tuberculatus and P. opisthoductus occur as vicarious species in the soil profile of the area wheretheir distribution overlaps. Three soil factors that are significant in determining earthworm distribution are emphasized:lessivage, or translocation of clay; the pervection of colloidal organic matter (humus into the soil, and the texture of the soillayers resulting from sedimentation. An additional record of the new species P. thaleri is given.

  9. "Da wurden diese zwei großen Länder zu einem Land". Die Beziehungen zwischen Hattusa und Ägypten im Lichte ihrer diplomatischen Korrespondenz

    OpenAIRE

    Quack, Joachim Friedrich

    2002-01-01

    Der ägyptisch-hethitische Friedensvertrag wurde nach der Schlacht bei Kadesch und jahrelangen Grenzstreitigkeiten im Jahr 21 der Regierungszeit Ramses II. zwischen Ramses II. und dem Hethiterkönig Hattusili III. geschlossen. Er gilt als ältester bekannter schriftlicher Friedensschluss.

  10. Evaluation of the dairy/yeast prebiotic, GroBiotic-A, in the diet of the juvenile Nile tilapia, Oreochromis niloticus

    Science.gov (United States)

    Tilapia production ranks among the highest worldwide due to their fast growth and ability to thrive under various culture conditions. However, tilapias are still susceptible to outbreaks of systemic disease caused by opportunistic bacterial pathogens such as Streptococcus inaie. These epizootic ev...

  11. Propiedades mecánicas de la mampostería de tabique rojo recocido utilizada en Chilpancingo, Gro (México)

    OpenAIRE

    V. Flores; Sánchez-Tizapa, S.; Arroyo, R.; Barragán, R.

    2013-01-01

    Experimental results of developed tests in solid clay brick masonry specimens are reported. This masonry type is largely used to build the confined masonry walls in Chilpancingo City (Guerrero State, Mexico). Sixty eight tests of masonry specimens, prisms and walls, were developed. Mean values of compressive and shear strength design, elasticity and shear modulus and joint mortar adherence were measured. Additionally, stress-strain relationship of compressive prisms and walls were recorded. C...

  12. Propiedades mecánicas de la mampostería de tabique rojo recocido utilizada en Chilpancingo, Gro (México

    Directory of Open Access Journals (Sweden)

    Flores, V.

    2013-09-01

    Full Text Available Experimental results of developed tests in solid clay brick masonry specimens are reported. This masonry type is largely used to build the confined masonry walls in Chilpancingo City (Guerrero State, Mexico. Sixty eight tests of masonry specimens, prisms and walls, were developed. Mean values of compressive and shear strength design, elasticity and shear modulus and joint mortar adherence were measured. Additionally, stress-strain relationship of compressive prisms and walls were recorded. Comparison among experimental results and standardized values of Mexico City Code, normally used to the design process, shows a large difference for some of them.Este documento presenta los resultados de ensayes en especímenes de mampostería de tabique rojo recocido comúnmente utilizado para la construcción de muros de mampostería confinada en Chilpancingo (Estado de Guerrero, México. Un total de 68 ensayes, entre pilas y muretes, fueron realizados. Los valores medidos fueron la resistencia de diseño a compresión y cortante, los módulos de elasticidad y corte y la cohesión de la junta. También se obtuvieron expresiones para definir la curva esfuerzo-deformación en las pruebas de pilas y muretes. La comparación de los valores respecto a la norma de la ciudad de México, utilizada como referencia en el diseño y análisis, concluye que hay diferencias significativas para algunos de ellos.

  13. Application of a relativistic accretion disc model to X-ray spectra of LMC X-1 and GRO J1655-40.

    OpenAIRE

    Gierliński, M.; Maciołek-Niedźwiecki, A.; Ebisawa, K.

    2001-01-01

    We present a general relativistic accretion disc model and its application to the soft-state X-ray spectra of black hole binaries. The model assumes a flat, optically thick disc around a rotating Kerr black hole. The disc locally radiates away the dissipated energy as a blackbody. Special and general relativistic effects influencing photons emitted by the disc are taken into account. The emerging spectrum, as seen by a distant observer, is parametrized by the black hole mass and spin, the acc...

  14. Einstein erlangt Weltruhm – Eine vergleichende Rezeptionsgeschichte der allgemeinen Relativitätstheorie in Großbritannien und Deutschland 1919-1920

    Directory of Open Access Journals (Sweden)

    Maik Hollinger

    2014-05-01

    Full Text Available Nowadays Albert Einstein is the archetype of a scientist. His world fame has its roots in the revolutionary corroboration of his general theory of relativity during the sun eclipse in May 1919 and the eventual presentation of the obtained results. Having a strong resonance in the scientific community as well as in public, the coverage in the press can be traced back to be the main factor that shaped Einstein’s picture in the past – reverberating even today. The political situation immediately after the First World War puts this episode in the history of science in a setting where many insights into the interactions between science, politics, the press and international relations at the shift from 1919–1920 can be found.

  15. Effects of dietary prebiotic GroBiotic®- A on growth performance, plasma thyroid hormones and mucosal immunity of great sturgeon, Huso huso (Linnaeus, 1758)

    DEFF Research Database (Denmark)

    Adel, M.; Nayak, S.; Lazado, Carlo Cabacang

    2016-01-01

    The present study was conducted to evaluate the effects of Grobiotic®-A, a commercial prebiotics, when administered in feed on the growth performance, plasma thyroid hormones and mucosal immunity of great sturgeon (Huso huso). The commercial prebiotic mixture was supplemented in the diets at four...... changes in growth performance parameters were observed, but only in those groups fed with 1% and 2% prebiotics. Specifically, marked improvements relative to the control group were observed in percentage weight gain, body weight gain, feed conversion ratio and specific growth rate in prebiotic-fed fish....... The levels of plasma thyroid hormones, specifically thyroxine and thyroid stimulating hormones were significantly elevated in the group receiving 2% prebiotics. Activities of lysozyme and alkaline phosphatase in skin mucus were significantly enhanced in prebiotics-fed groups, particularly at an inclusion...

  16. "Ein großer Vorsprung gegenüber Deutschland". Die niederländischen Architekten auf der Bauhausausstellung von 1923 in Weimar

    Directory of Open Access Journals (Sweden)

    van Bergeijk, Herman

    2013-01-01

    Full Text Available This article discusses the Dutch contribution to the International Architecture Exhibition that was part of the Bauhaus Manifestation in 1923. The Dutch architect J.J.P. Oud, together with the director of the school in Weimar, Walter Gropius, decided which architects would be asked to participate and send in works. Oud selected the works of these architects according to his own criteria. He had a specific agenda that was dictated by the position he had in the Netherlands and abroad as an advocate of modernist architecture. His interest was not to show what was really going on in general in the Netherlands but to illustrate that there was a strong movement in the country towards the development of a new architecture based on the use of new materials. The article positions this radical attitude of Oud and confronts it with how German architects, who were particularly informed, looked at the situation in the neighbouring country.

  17. Radi On Tibet

    Institute of Scientific and Technical Information of China (English)

    1994-01-01

    Radi On TibetRadiOnTibetWhatisthecurrentsituationinTibet?WillTibetenjoypeaceandstabilityforthelongterm?Ourstaffreportmoreduri...

  18. DEGRO 2006: 12. annual congress of the German Radiation Oncology Society, 10. annual meeting of SASRO and 22. annual meeting of OeGRO. Abstracts; DEGRO 2006 - 12. Jahreskongress der Deutschen Gesellschaft fuer Radioonkologie, 10. Jahrestagung der SASRO und 22. Jahrestagung der OeGRO. Abstracts

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2006-05-15

    The volume displays the abstracts of the meetings of the German, Swiss, and Austrian societies for radiooncology. Issues of the lectures, posters, and symposia were: new technological developments, head-neck tumors, radiobiology, prostate carcinomas, radiotherapy in palliative treatment, hematological malignomas, tumors of gastrointestinal tract, bladder, brain, breast, or bronchi, carrier planning, women in the field of radiooncology, image-guided radiotherapy, sexuality, fertility and pregnancy with gynecological tumors, psychooncology, pediatric oncology and radiotherapy, sex-dependent radiosensitivity, dosimetry, irradiation planning, quality of life in radiooncology, radiooncological information systems. (uke)

  19. Voina radi jevro / Jevgenija Garanzha

    Index Scriptorium Estoniae

    Garanža, Jevgenija, 1979-

    2004-01-01

    Alanud aastal kavatseb Eesti valitsus tõsiselt tegelda jooksevkonto defitsiidiga, kuna majandusliku arengu pidurdamise kõrval takistab see ka meie ühinemist ühtse Euroopa valuutaga. Diagrammid: Majandusnäitajad 2003. a. - majanduskasv, inflatsioon, jooksevkonto defitsiit

  20. Leitsymptomvorlesungen im klinischen Studienabschnitt - Effekte evaluationsbasierter Interventionen auf eine Großgruppen-Lehrveranstaltung [Lectures based on cardinal symptoms in undergraduate medicine - effects of evaluation-based interventions on teaching large groups

    Directory of Open Access Journals (Sweden)

    Kuhnigk, Olaf

    2011-02-01

    Full Text Available [english] Despite critical voices lectures are still an important teaching format in current medical curricula. With the curricular reform at Hamburg Medical Faculty in the year 2004, all subject specific lectures were replaced by cardinal symptom oriented lectures (LSV in the new clinical curriculum. LSVs are taught throughout all six thematic blocks in years three to five. Since regular student evaluations after each thematic block seemed to demand improvement of the LSVs, this study was carried out using evaluations of individual LSVs by the participating students and by trained auditors (final year students and academic staff. Based on these evaluations feedback containing the individual evaluation data was given in written form to the lecturers combined with information material on planning an LSV using modern didactic techniques. In a second evaluation period, the effects of this intervention were studied. Only small improvements in the LSVs’ quality were noted regarding the level of marks achieved. When individual items were evaluated, especially the didactic quality, significant improvements were noticeable. Overall, on the basis of individual items students ranked the quality of the LSVs significantly higher than trained auditors during the first evaluation period. This effect was no longer seen after the second evaluation period. The inter rater reliability among the auditors was very good. This study shows that regular quality assurance is needed on the structural levels and for staff to accompany the process of embedding teaching formats into curricular concepts. Further investigation is needed to determine the adequate frequency of evaluation and the format of feedback to guarantee sustainable effects of the didactic quality of lectures. [german] In der medizinischen Hochschulausbildung ist das Veranstaltungsformat „Vorlesung“ trotz vielfacher Kritik weiterhin ein wesentliches Element bestehender Curricula. Im Rahmen der Studienreform an der Medizinischen Fakultät der Universität Hamburg im Jahr 2004 wurden im reformierten klinischen Curriculum die fachspezifischen Vorlesungen durch Leitsymptomvorlesungen (LSV ersetzt, die sich durch alle sechs Themenblöcke der Studienjahre drei bis fünf ziehen. Da die regelmäßigen Trimesterabschlussevaluationen der Studierenden auf einen Verbesserungsbedarf der LSV hindeuteten, wurde in dieser Studie die LSV mit Terminevaluationen durch anwesende Studierende und durch geschulte Auditoren (PJ-Studierende und wissenschaftliche Mitarbeiter untersucht. Auf der Basis dieser Ergebnisse erfolgte ein schriftliches Feedback der Evaluationsdaten an die Lehrenden in Kombination mit Informationsmaterial über eine optimale Gestaltung der LSV nach modernen didaktischen Gesichtspunkten. In einer zweiten Erhebung wurden die Effekte der Intervention untersucht. Es zeigte sich, dass auf der Ebene von Schulnoten nach der Intervention nur geringe Verbesserungen der Qualität der LSV bemerkbar waren. In der Bewertung der Einzelmerkmale, insbesondere zur didaktischen Qualität, ließen sich hingegen signifikante Verbesserungen aufzeigen. Insgesamt bewerteten Studierende die LSV in der ersten Erhebungsphase bezogen auf die Einzelmerkmale signifikant positiver als die geschulten Auditoren. Dieser Effekt war in der zweiten Erhebungsphase nicht mehr nachweisbar. Unter den Auditoren bestand eine gute Inter-Rater-Reliabilität. Durch diese Untersuchung wurde insbesondere deutlich, dass die Einbettung von Lehrveranstaltungen auf struktureller und personeller Ebene in curriculare Gesamtkonzepte regelmäßig durch Qualitätssicherungsmaßnahmen zu begleiten ist. Wie häufig und in welcher Form strukturierte Rückmeldungen nach Evaluationen erfolgen müssen, um nachhaltige Effekte auf die didaktische Qualität der Lehrveranstaltungen zu haben, müssen zukünftige Studien zeigen.

  1. Wprowadzenie tzw. specustawy dotyczącej kierowania groźnych przestępców do ośrodków terapii osobowości – kontekst społeczno-prawny

    Directory of Open Access Journals (Sweden)

    Błażej Kmieciak

    2013-12-01

    Full Text Available Ustawa o postępowaniu wobec osób z zaburzeniami psychicznymi stwarzających zagrożenie życia, zdrowia lub wolności seksualnej innych osób to jeden z najbardziej krytykowanych w ostatnich miesiącach aktów prawnych. Liczne grono prawników oraz psychiatrów wskazuje, iż proponowane rozwiązania są sprzeczne ze standardami ochrony praw człowieka, które wiele lat temu zostały przyjęte przez Polskę. Podkreśla się ponadto, że przyjęcie ustawy otworzy drogę do wykorzystywania psychiatrii do celów politycznych. Z drugiej strony osoby broniące przywołaną ustawę twierdzą, że część osadzonych osób, z powodu doświadczania zaburzeń osobowości bądź zaburzeń preferencji seksualnych, zamierza powrócić do działań przestępczych, skierowanych często przeciwko życiu i zdrowiu dzieci. Należy wspomnieć, iż pod koniec lat 80. XX wieku podobne działania doprowadziły do orzeczenia kary śmierci wobec ww. osób. Co istotne, przez ponad 20 lat nie podejmowano tematu sytuacji więźniów, którym karę śmierci następnie zamieniono na karę 25 lat pozbawienia wolności. Czy prawdą jest zatem, iż omawiana ustawa łamie fundamentalne prawa i wolności człowieka? Czy istnieje realna obawa wykorzystywania przywołanego aktu prawnego do celów politycznych? Czy psychiatrzy mogą zostać zobowiązani do wykonywania zadań wyłącznie o politycznym charakterze? Czy obecnie nie ma innego rozwiązania zapewniającego poszanowanie bezpieczeństwa publicznego? W prezentowanym artykule odpowiedzi na powyższe pytania będą udzielane na podstawie analizy oficjalnych materiałów prawnych oraz medialnych informacji i komentarzy odnoszących się do omawianej ustawy.

  2. Groin sentinel node biopsy and (18)F-FDG PET/CT-supported preoperative lymph node assessment in cN0 patients with vulvar cancer currently unfit for minimally invasive inguinal surgery: The GroSNaPET study.

    Science.gov (United States)

    Garganese, G; Collarino, A; Fragomeni, S M; Rufini, V; Perotti, G; Gentileschi, S; Evangelista, M T; Ieria, F P; Zagaria, L; Bove, S; Giordano, A; Scambia, G

    2017-09-01

    The study aims were: 1) to verify the role of sentinel node biopsy (SNB) in a subset of patients with clinical N0 (cN0) invasive vulvar cancer (VC) who were still candidates for radical inguinal surgery according to the current guidelines; 2) to investigate whether a preoperative (18)F-FDG PET/CT (PET/CT) evaluation could improve the selection of node negative patients. From July 2013 to July 2016, all patients with VC admitted to our Division were evaluated by standard imaging and clinical exam. Among the patients assessed as cN0 we enrolled those unsuitable for SNB, due to: T > 4 cm, multifocal tumors, complete tumor diagnostic excision, contralateral nodal involvement and local recurrence. A preoperative PET/CT was performed. For each patient surgery included SNB, performed using a combined technique (radiotracer plus blue dye), followed by standard inguino-femoral lymphadenectomy. The reference standard was histopathology. Forty-seven patients entered the study for a total of 73 groins. Histopathology revealed 12 metastatic SNs in 9 groins. No false negative SNs were found (NPV 100%). PET/CT showed a negative predictive value of 93%. Our data suggest that SNB is accurate and safe even in cN0 patients currently excluded from this procedure, providing that a careful preoperative selection is performed. PET/CT allows a reliable assessment of LN status and may be an effective support for the selection of patients who are safe candidates for SNB. Copyright © 2017 Elsevier Ltd, BASO ~ The Association for Cancer Surgery, and the European Society of Surgical Oncology. All rights reserved.

  3. Protein (Cyanobacteria): 497242008 [PGDBj - Ortholog DB

    Lifescience Database Archive (English)

    Full Text Available 2:1589 ... Co-chaperonin GroES Oscillatoriales cyanobacterium JSC-12 MNFQSHALLFHFCRILSSPHPPKERRGAKPFQSPSNKLGEGLRARAKVKCTRTDNGIFLPASVQEKPQVGEITAISSGKFSKNGSGQQADVKVGDNILYSMYRGTEIYLGNERM

  4. FORMATION AND QUANTIFICATION OF PROTEIN COMPLEXES BETWEEN PEROXISOMAL ALCOHOL OXIDASE AND GROEL

    NARCIS (Netherlands)

    EVERS, ME; LANGER, T; HARDER, W; HARTL, FU; VEENHUIS, M; Hartl, Franz-Ulrich

    1992-01-01

    We have studied the use of yeast peroxisomal alcohol oxidase (AO) as a model protein for in vitro binding by GroEL. Dilution of denatured AO in neutral buffer leads to aggregation of the protein, which is prevented by the addition of GroEL. Formation of complexes between GroEL and denatured AO was d

  5. Using a field radiometer to estimate instantaneous sky clearness Radiômetro de campo para cálculo da clareza instantânea do céu

    Directory of Open Access Journals (Sweden)

    Eduardo G. Souza

    2006-06-01

    Full Text Available Reflectance measurements of crop plants and canopies show promise for guiding within-season, variable-rate nitrogen (N application. Most research results have been obtained around solar noon with clear skies. However, for practical application, the system must work under cloudy skies or away from solar noon. The objective of this work was to assess the effect of cloud conditions on reflectance measurements of a corn canopy. The approach was to estimate an instantaneous sky clearness index (ICI which could be used to correct field radiometer data for variations in cloud cover, such that the same reflectance reading would be obtained (and the same N recommendation made for the same plants regardless of cloud conditions. Readings were taken from morning until night over 11 days with a range of sky conditions (sunny, overcast, partly cloudy. Data from clear days were used to estimate the theoretical expected spectral global radiation incident on a horizontal surface. The ICI was calculated as the ratio between the actual spectral global radiation and the corresponding theoretical global radiation. Analysis of the ICI for each band showed that the influence of cloudiness was different for each band. Thus, the cloud effect could not be compensated by the use of a band ratio or vegetation index.Medidas da reflectância das folhas das plantas mostram-se promissoras para a aplicação de nitrogênio a taxa variável; entretanto, a maioria dos resultados de pesquisa foi obtida ao redor do meio-dia solar e com céu aberto, porém para aplicações práticas um sistema tem que trabalhar debaixo de céu nublado e fora do meio-dia solar. O objetivo deste trabalho foi avaliar o efeito de condições de nuvem em medidas de reflectância de milho. A abordagem foi calcular um índice instantâneo de clareza do céu (ICI que pode ser usado para corrigir dados de radiômetros de campo para variações em cobertura de nuvem, tal que essas reflectâncias seriam

  6. In-situ X-ray CT results of damage evolution in L6 ordinary chondrite meteorites

    Energy Technology Data Exchange (ETDEWEB)

    Cuadra, Jefferson A. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Hazeli, Kavan [Univ. of Alabama, Huntsville, AL (United States); Ramesh, K. T. [Johns Hopkins Univ., Baltimore, MD (United States). Hopkins Extreme Materials Inst.; Martz, Harry [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Nondestructive Characterization Inst.

    2016-06-17

    These are slides about in-situ X-ray CT results of damage evolution in L6 ordinary chondrite meteorites. The following topics are covered: mechanical and thermal damage characterization, list of Grosvenor Mountain (GRO) meteorite samples, in-situ x-ray compression test setup, GRO-chipped reference at 0 N - existing cracks, GRO-chipped loaded at 1580 N, in-situ x-ray thermal fatigue test setup, GRO-B14 room temperature reference, GRO-B14 Cycle 47 at 200°C, GRO-B14 Cycle 47 at room temperature, conclusions from qualitative analysis, future work and next steps. Conclusions are the following: Both GRO-Chipped and GRO-B14 had existing voids and cracks within the volume. These sites with existing damage were selected for CT images from mechanically and thermally loaded scans since they are prone to damage initiation. The GRO-Chipped sample was loaded to 1580 N which resulted in a 14% compressive engineering strain, calculated using LVDT. Based on the CT cross sectional images, the GRO-B14 sample at 200°C has a thermal expansion of approximately 96 μm in height (i.e. ~1.6% engineering strain).

  7. Multiple Beneficial Health Effects of Natural Alkylglycerols from Shark Liver Oil

    Directory of Open Access Journals (Sweden)

    Alain B. Legrand

    2010-07-01

    Full Text Available Alkylglycerols (alkyl-Gro are ether lipids abundant in the liver of some elasmobranch fish species such as ratfishes and some sharks. Shark liver oil from Centrophorus squamosus (SLO, or alkyl-Gro mix from this source, have several in vivo biological activities including stimulation of hematopoiesis and immunological defences, sperm quality improvement, or anti-tumor and anti-metastasis activities. Several mechanisms are suggested for these multiple activities, resulting from incorporation of alkyl-Gro into membrane phospholipids, and lipid signaling interactions. Natural alkyl-Gro mix from SLO contains several alkyl-Gro, varying by chain length and unsaturation. Six prominent constituents of natural alkyl-Gro mix, namely 12:0, 14:0, 16:0, 18:0, 16:1 n-7, and 18:1 n-9 alkyl-Gro, were synthesized and tested for anti-tumor and anti-metastatic activities on a model of grafted tumor in mice (3LL cells. 16:1 and 18:1 alkyl-Gro showed strong activity in reducing lung metastasis number, while saturated alkyl-Gro had weaker (16:0 or no (12:0, 14:0, 18:0 effect. Multiple compounds and mechanisms are probably involved in the multiple activities of natural alkyl-Gro.

  8. Characterization of the transcriptional regulation of the tariJKL locus involved in ribitol-containing wall teichoic acid biosynthesis in Lactobacillus plantarum

    NARCIS (Netherlands)

    Tomita, S.; Lee, I.C.; Swam, van I.I.; Boeren, S.; Vervoort, Jacques; Bron, P.A.; Kleerebezem, M.

    2016-01-01

    Lactobacillus plantarum strains produce either glycerol (Gro)- or ribitol (Rbo)-backbone wall teichoic acid (WTA) (Gro-WTA and Rbo-WTA, respectively). The strain WCFS1 has been shown to be able to activate the tarIJKL locus involved in Rbo-WTA synthesis when the tagD1F1F2 locus for Gro-WTA synthe

  9. Interfacial air/water proton conduction from long distances by sulfolobus solfataricus archaeal bolaform lipids.

    Science.gov (United States)

    Vilalta, I; Gliozzi, A; Prats, M

    1996-08-15

    The stability, structural organization, and the ability to transfer protons long distances have been investigated in monolayers formed from archael bolaform lipids at the air/water interface. The lipids employed were the fractions GroR2Gro (R represents an acyl group with variable chain length typically consisting of 0-4 cyclopentane rings and 40 isoprenoid residues) and GroR2GroNon-Ol (Non-ol represents nonitol) extracted from Sulfolobus solfataricus by hydrolysis of the cytoplasmic membrane. GroR2-GroNon-ol films exhibit a very peculiar behaviour: the monolayer surface pressure increases with time, regardless of its low or high initial value. This finding is related to the possibility of GroR2GroNon-ol molecules to assume an upright (a metastable) or a U-shaped (stable) configuration. In the gaseous state and in the collapsed state of the film, no lateral proton conduction was observed. However, in the pressure range 0 < pi < 25 mN/m for GroR2Gro and 0 < pi < 30 mN/m for GroR2GroNon-ol monolayers, a lateral proton conduction at the air/water interface was observed. The structural organization of these bipolar lipids at the air/water interface can be related to the lateral proton conduction; it is possible to conclude that whatever configuration these lipids may adopt, they are able to structure the air/water interface in a hydrogen bond network that supports lateral proton conduction. This process may be ascribed to a percolation phenomenon occurring when the polar lipid head groups form a structured lattice of hydrogen bonds.

  10. The clonal antibody response to Pseudomonas aeruginosa heat shock protein is highly diverse in cystic fibrosis patients

    DEFF Research Database (Denmark)

    Ulanova, M; Petersen, T D; Ciofu, O;

    1997-01-01

    The GroEL protein of Pseudomonas aeruginosa belongs to the bacterial 60-65 kDa heat shock protein family. A strong antibody response to GroEL has been found in cystic fibrosis (CF) patients with chronic pulmonary infection caused by P. aeruginosa. Clonotypes of IgG1 and IgG2 antibodies against Gro...... antibody clones against GroEL. The appearance of new clones with time reflected the long duration of the chronic infection. A striking addition of new clonotypes during the observation period occurred when a new unrelated bacterium (Burkholderia cepacia) had become established as a cause of the pulmonary...

  11. Ultrasound irradiation: a robust approach for direct functionalization of graphene oxide with thermal and antimicrobial aspects.

    Science.gov (United States)

    Maktedar, Shrikant S; Mehetre, Shantilal S; Singh, Man; Kale, R K

    2014-07-01

    Sonochemical waves as mechanochemical energy was employed to exfoliate graphite oxide and functionalized graphene oxide (GrO), through a reaction of solvent and accountable for top-down and bottom-up approach respectively. The in situ formation of ester intermediate was inferred and a polymeric surface of GrO was further functionalized with 6-Aminoindazole (6-AIND) through sonochemical nucleophilic substitution reaction. As compared to conventional method the effect of ultrasound was verified for the direct functionalization of GrO. The conventional hazardous acylation step for functionalization of GrO was deleted in ultrasound assisted formation of f-(6-AIND) GrO nanocomposite, prepared by stereoselective exploitation of carboxyl groups at edges of GrO. The characterization has ascertained a covalent attachment of 6-AIND onto GrO surface with ATR-FTIR, XPS, SSNMR, TGA, DSC, XRD, AFM, RAMAN, EDX, SEM, BET and elemental analyzer. A weight loss in TGA depicts enhanced thermal stability of f-(6-AIND) GrO and a thermally sensitive behavior. The f-(6-AIND) GrO was studied for in vitro antimicrobial activity to ensure health and environmental safety. Antibacterial activity was identified against human pathogenic gram-positive (Staphylococcus aureus; ATCC 25923) and gram-negative bacteria (Escherichia coli; ATCC 25922). The antifungal activity was observed against Candida albicans (ATCC 10231).

  12. Efecto del hidrogel sobre el rendimiento de semilla en tres cultivares de Brachiaria spp en el valle de Iguala, gro., México (Efect of hidrogel on yield of seed of three cultivars of Brachiaria ssp in the valley of Iguala guerrero, México

    Directory of Open Access Journals (Sweden)

    Bartolo Hernández Santana

    2007-09-01

    Full Text Available ResumenBajo un diseño de bloques completamente al azar en un arreglo de parcelas divididas, se evaluó el efecto de tres niveles de hidrogel sobre el rendimiento de semilla de tres cultivares de Brachiaria ssp . Los niveles fueron 0,10 y 20 Kg ha-¹ de hidrogel, y los cultivares; Insurgente, Mulato y Toledo. Las variables consideradas fueron: altura de planta al 100% de floración (APF,densidad de tallos (DT, densidad de tallos reproductivos primarios (DTRP,densidad de tallos reproductivos secundarios (DTRS, porcentaje de acame (PA, rendimiento de semilla bruta (RSBR, rendimiento de semilla ventilada (RSVE y rendimiento de semilla pura (RSPU. En el análisis estadístico a todas las variables se les aplicó el análisis de varianza para evaluar el efecto del hidrogel y el comportamiento de los cultivares en el rendimiento de semilla. Se efectuaron las pruebas de comparación múltiple de medias de Tukey para conocer si existió asociación de las variables de estudio. El análisis de varianza mostró que existió diferencias significativas (P <0.05 para el factor cultivar en las variables: (RSVE, (RSPU, (DT, (DTRP y (PA y altamente significativas (P<0.01 en (RSBR, (APF y (DTRS. En relación al factor hidrogel no se encontraron diferencias estadísticas en ninguna de las variables estudiadas. En tanto que en la interacción cultivar por hidrogel sólo la variable (DT presentó diferencias significativas (P<0.05. La comparación múltiple de medias evidenció que existió diferencias estadísticas entre cultivares en todas las variables de estudio, destacando que en la variable (RSBR, el cv Mulato fue el que presentó la mejor media con 516.06 Kg de semilla ha-¹ mientras que para las variables (RSVE y (RSPU fue el cv Toledo, con 88.36 y 82.45 Kg ha-¹ respectivamente. En relación a los niveles de hidrogel no se encontró diferencias estadísticas en ninguna de las variables de estudio.AbstractIt was evaluated the effect of three hidrogel levels on the yield of seed of three cultivars of Brachiaria ssp. This experiment was done in a random design in blocks. The hidrogel levels were 0, 10, and 20 kg ha-¹ and cultivars were Insurgente, Mulato and Toledo. The variables considered for yield were: plant height is when 100% blooming, stem density, primary reproductive stem density, secondary reproductive stem density, fallen plants porcentage, gross seed yield, ventilated seed yield and pure seed yield. An ANOVA was applied to the variables to evaluate the effect of the hidrogel in the cultivars measuring the yield and seed.Tukey multiple comparison test were done to know the variables relation in this study. The ANOVA showed significative differences (P < 0.05 for the cultivar in the variables: ventilated seed yield, pure seed yield, stem density, primary reproductive stem density, fallen plants porcentage, also highly significative (P<0.01 for gross seed yield, height plant in 100% blooming, secundary reproductive stem density. The variable stem density showed significative differences with the hidrogel – cultivar interaction. Tukey multiple comparison showed the differences in the cultivar variables indicating that the variable gross seed yield in the Mulato cultivar presented the best mean with 516.06 Kg ha-¹ of seed and the variables ventilated seed yield and pure seed yield for Toledo cultivar presented the best means with 88.36 and 82.45 Kg ha-¹ of seed respectively. The hidrogel levels not showed significative differences (P<0.05 on the variables considered.

  13. Wirklich wie im wirklichen Leben. Sarah Kember untersucht die (Re-Produktion des kleinen Unterschieds und seiner großen Folgen in Theorie und Praxis von Artificial Life Real as in real life. Sarah Kember examines the (reproduction of ‘the small difference and its big consequences’ in the theory and practice of artificial life.

    Directory of Open Access Journals (Sweden)

    Verena Kuni

    2004-03-01

    Full Text Available Wie greifen diese ‚virtuellen Realitäten‘ in unsere Körper- und Identitätskonzepte, unsere Subjekt- und Geschlechtervorstellungen ein? Sarah Kembers Buch verspricht, erhellende Schneisen durch das Dickicht der definitionsmächtigen Diskurse, Konzepte und Konstruktionen zu schlagen und neue Wege für feministische Interventionen in die Auseinandersetzungen um ‚Artificial Life‘ aufzuzeigen.ow a way through the labyrinth of discourse, concepts and constructions, and to open the door for feminist interpretations in debates about artificial life.

  14. Ernst Robert Curtius: Evropska literatura in latinski srednji vek. Prevedel Tomo Virk, latinske in grške citate prevedla Nada Grošelj. Ljubljana: Literarno-umetniško društvo Literatura, 2002. (Zbirka Labirinti 582 str. (review

    Directory of Open Access Journals (Sweden)

    Nada Grošelj

    2003-07-01

    Full Text Available Po več kot pol stoletja smo dočakali slovenski prevod enega temeljnih literarnovednih del 20. stoletja, Europaische Literatur und lateinisches Mittelalter (1948. Njegov avtor Ernst Robert Curtius (1886-1956 je bil v prvi vrsti romanist, vendar sta mu širina intelektualnih zanimanj in erudicija omogočali, daje v svoje interpretacije zajemal poleg romanskih tudi angloameriške in nemške sodobnike ter srednjeveške in antične pisce.

  15. 麻疯树(Jatrophacurcas)叶浸提液对万寿菊(TageteserectaL.)幼苗生长和抗氧化酶活性的影响%Allelopathic Effects of Jatropha curcas Leaf Leachates on Seedling Gro wth and Antioxidative Enzymes in Tagetes erecta L.

    Institute of Scientific and Technical Information of China (English)

    张红; 王进闯

    2011-01-01

    [目的]研究麻疯树叶浸提液对万寿菊幼苗生长和抗氧化酶活性的化感影响,揭示麻疯树对林下植物的化感生理机制。[方法]对麻疯树叶浸提液对万寿菊幼苗的生长、丙二醛(MDA)、脯氨酸(Proline)和抗氧化酶系统的影响进行研究和分析。[结果]与对照相比,麻疯树叶浸提液加入土壤后,明显抑制了万寿菊根和茎的生长;同时,在叶浸提液中,万寿菊根中的O2-、H2O2、丙二醛和脯氨酸的含量升高,且浸提液浓度的增加而增加;另外,麻疯树叶浸提液在较低浓度时,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性无显著变化,而在较高浓度时,SOD酶活性明显增加;所有浓度的叶浸提液都显著增加了万寿菊根中过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,且随着浓度的增加而增加。[结论]麻疯树能释放化感物质进入土壤,抑制植物的生长和生理,改变植物群落组成和结构。%[Objective] This study was to investigate the allelopathic effects of J.curcas leaf leachates on seedling growth and antioxidative enzymes,and thus to reveal physiologically allelopathic effects of J.curcas trees on undergrowth plants.[Method] The allelopathic effects of leaf leachates of Jatropha curcas amended into soil were determined on the growth,proline and malondialdehyde(MDA) contentas well as superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX),guaiacol peroxidase(POD) in roots of marigold(Tagetes erecta) seedlings.[Results] The application of leaf leachates of J.curcas in the soil significantly inhibited the shoot and root length of marigold compared to un-amended soils.The leaf leachates increased the O-2,H2O2,MDA and proline content in the roots of marigold seedlings.The effects increased with the ascending of leaf leachates concentration.In addition,superoxide dismutase(SOD) and guaiacol peroxidase(POD) were not remarkably affected by the leachates at lower concentrations,but at higher concentration,SOD activity was ascended.Similarly,the activities of CAT and APX were also significantly increased with the increase of leachates concentrations.[Conclusion] These results show that J.curcas can release the phytotoxic compound(s) into soil,which are inhibitory to certain test species by interfering with physiological process.

  16. Effect of p erio dic gro oving top ography on dynamics of Insoluble surfactant-laden thin film flow%连续凹槽基底对含非溶性活性剂薄液膜流动特性的影响

    Institute of Scientific and Technical Information of China (English)

    李春曦; 陈朋强; 叶学民

    2014-01-01

    针对连续凹槽基底上含非溶性活性剂液膜的流动过程,采用润滑理论建立了液膜厚度和活性剂浓度演化模型,利用PDECOL程序数值模拟得到了液膜流动的动力学特征及基底结构参数的影响。研究表明:活性剂液膜流经连续凹槽时,负向台阶处形成凹陷,正向台阶处形成隆起,且随时间逐渐增大;与平整基底相比,连续凹槽下的活性剂液膜铺展速度加快;基底凹槽的高度增加或斜度减小可加速液膜破断的可能性;增大凹槽宽度可促进液膜流动;减小斜度会使液膜进入第一凹槽前形成隆起特征;重力在液膜的爬坡和下坡过程中具有相反的作用,但均使得流动稳定性变差;分子间作用力形成的结合压可加速液膜流动,进而引发去湿润现象,分离压则与之相反。%Targeting the flowing of insoluble surfactant-laden film over topography substrate, the lubrication theory is adopted to derive the evolution equations of thin liquid film thickness and interfacial surfactant concentration. The flowing characteristics of the film on topography surfaces, and the influence of topography structure are examined based on the numerical simulation with PDECOL code. The results show that when the thin film of insoluble surfactant flows over periodic grooving topography, the depression appears at the negative step, while the ridge is shaped at the positive step, both of which increase gradually with time going by. Compared with the case of the flat base, the surfactant-laden film spreading speed is enhanced. Increasing the groove depth or reducing the groove steepness leads to the increase of the rupture possibility of the film. Improving the groove width promotes the film flowing. The decrease of the steepness can cause the film to form a ridge feature before entering into the first groove. Gravity has the opposite effects on the up-hilling and down-hilling processes of liquid film flow, which causes the flow stability to deteriorate. Conjoining pressure induced by intermolecular forces can accelerate the film, giving rise to a notable dewetting phenomenon, while disjoining pressure has an adverse effect.

  17. Effect of curve gro ove on the sp ectral resolution for soft X-ray holographic flat-field gratings%软X射线全息平焦场光栅的条纹弯曲现象及其对光谱分辨率的影响∗

    Institute of Scientific and Technical Information of China (English)

    陈火耀; 刘正坤; 王庆博; 易涛; 杨国洪; 洪义麟; 付绍军

    2014-01-01

    软X射线平焦场光栅光谱仪是等离子体诊断的重要仪器,其核心光学元件全息平焦场光栅通常采用非球面波记录光路制作,因此光栅条纹存在弯曲的现象.光栅条纹的弯曲会影响光谱成像质量,从而影响系统的光谱分辨率.记录光路的优化,只保证光栅子午面的线密度分布,因此优化的记录光路并不是惟一的,所以在保证子午面的线密度分布的同时能制作具有不同弯曲程度条纹的光栅.针对应用于0.8—6 nm的全息平焦场光栅,利用光线追迹方法分析了不同弯曲程度条纹光栅的光谱成像,发现采用柱面反射镜制作的接近于直条纹的光栅具有较好的光谱成像质量.相对于弯曲条纹的光栅,接近于直条纹的光栅理论光谱分辨率有明显的提高,入射波长为3 nm时,光谱分辨率从626提升到953,入射波长为5 nm时,光谱分辨率从635提高到1222.%The soft X-ray spectrograph is an important instrument for plasma diagnostics. As the core optical element of spectrograph, holographic flat-field grating is fabricated by aspheric wave-front recording optics, so grooves on the surface are curve. The curve grooves of the grating would affect the spectral image properties, thus influencing spectral resolutions. In the design of recording optics, only the groove density distribution on the surface in meridian line should be guaranteed, so optimized recording optics is not unique. Thus gratings with different curvatures of grooves but with expected groove density distribution could be obtained. For holographic flat-field gratings used in a 0.8–6 nm region, we analyze the influences of different curve grooves on the spectral image by ray tracing, and find that the almost straight grooves which are obtained by means of cylinder mirror can obtain the better spectral images. The theoretical results show that the spectral resolutions of grating with almost straight grooves are obviously improved compared with curve grooves, the theoretical spectral resolutions increase from 626 to 953 at 3 nm and from 635 to 1222 at 5 nm, respectively.

  18. Engineering a nanopore with co-chaperonin function

    NARCIS (Netherlands)

    Ho, Ching-Wen; Meervelt, Veerle; Tsai, Keng-Chang; De Temmerman, Pieter-Jan; Mast, Jan; Maglia, Giovanni

    2015-01-01

    The emergence of an enzymatic function can reveal functional insights and allows the engineering of biological systems with enhanced properties. We engineered an alpha hemolysin nanopore to function as GroES, a protein that, in complex with GroEL, forms a two-stroke protein-folding nanomachine. The

  19. Molecular and biochemical characterization of a new thermostable bacterial laccase from Meiothermus ruber DSM 1279

    DEFF Research Database (Denmark)

    Kalyani, D. C.; Munk, L.; Mikkelsen, J. D.

    2016-01-01

    A new laccase gene (mrlac) from Meiothermus ruber DSM 1279 was successfully overexpressed to produce a laccase (Mrlac) in soluble form in Escherichia coli during simultaneous overexpression of a chaperone protein (GroEL/ES). Without the GroEL/ES protein, the Mrlac overexpressed in E. coli...

  20. NCBI nr-aa BLAST: CBRC-PTRO-10-0009 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-10-0009 ref|YP_981446.1| Alcohol dehydrogenase GroES domain protein [Polar...omonas naphthalenivorans CJ2] gb|ABM36525.1| Alcohol dehydrogenase GroES domain protein [Polaromonas naphthalenivorans CJ2] YP_981446.1 4.6 33% ...

  1. Die grossen Physiker und ihre Entdeckungen von den fallenden Körpern zu den Quarks

    CERN Document Server

    Segrè, Emilio

    1998-01-01

    Von Galileo Galilei bis zu Richard Feynman und Murray Gell-Mann - von den fallenden Körpern zu den Quarks: Der Physiknobelpreisträger Emilio Segre hat seine ganz persönliche Geschichte der Physik geschrieben. Er erzählt von den großen Gestalten und deren wichtigen Entdeckungen mit großer Anschaulichkeit und Lebendigkeit.

  2. Dicty_cDB: Contig-U15272-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .0 2 ( AB242233 ) Lettuce yellows phytoplasma groES, groEL, amp, na... 34 7.0 2 ( AB124810 ) Paulownia witch...Clover phyllody phytoplasma immunodominant membra... 34 7.4 2 ( DQ515794 ) Paulow

  3. Teilansicht der Universitätssternwarte Wien: Die Wiener Sternwarte

    OpenAIRE

    Kerschbaum, Franz

    2005-01-01

    Die Detailansicht zeigt den Eingangsbereich mit seinem großen Glasdach. Süden ist rechts. Unter dm Glasdach befindet sich die große Treppe, über die man in die eigentliche Sternwarte gelangt. Das Foto wurde vom weinhauser Kirchturm aufgenommen. Fotograf: Prof. Franz Kerschbaum

  4. Synthesis and folding of a mirror-image enzyme reveals ambidextrous chaperone activity

    National Research Council Canada - National Science Library

    Matthew T. Weinstock; Michael T. Jacobsen; Michael S. Kay

    2014-01-01

    .... Impressively, GroEL/ES folds both L-and D-DapA. This work extends the limits of chemical protein synthesis, reveals ambidextrous GroEL/ES folding activity, and provides a valuable tool to fold D-proteins for drug development and mirror-image synthetic biology applications.

  5. Dopustimo li ubistvo radi iskusstva? / Jüri Arrak

    Index Scriptorium Estoniae

    Arrak, Jüri, 1936-

    2005-01-01

    Artiklite "Kas kunsti nimel võib tappa?", "Sõnavõtt Eesti kultuurikonverentsil" ning "Muust ja sitast" tõlked Jüri Arraku esseekogumikust "Võsa, aas ja mägi" (Tallinn, 2003). Mõtteid kunsti nimel tapmise lubatavusest seoses taani shokikunstniku B. Norgaardi näitusega "Uus Euroopa" Tallinnas. Usu osast ja vaimsusest kunstis. Kriitikat seoses ekskrementide demonstreerimisega kunstina

  6. Podaite, Hrista radi, 150 mln. kron / Artur Tooman

    Index Scriptorium Estoniae

    Tooman, Artur, 1971-

    2006-01-01

    Keskkonnaministeerium valmistab ette seaduseprojekti nn "nafta fondi" loomiseks võimaliku naftareostuse likvideerimise finantseerimiseks. Sadamaoperaatorid on nördinud riigi kavatsusest lahendada oma probleemid ettevõtjate arvel, keskkonnaministeeriumi esindaja sõnul ei ole veel otsustatud, kes ja kui palju peaks maksma hakkama

  7. Understanding the mechanism of surface modification through enhanced thermal and electrochemical stabilities of N-doped graphene oxide

    Energy Technology Data Exchange (ETDEWEB)

    Mehetre, Shantilal S., E-mail: shantilalmehetre@gmail.com; Maktedar, Shrikant S., E-mail: shrikantmaktedar@gmail.com; Singh, Man, E-mail: mansingh50@hotmail.com

    2016-03-15

    Highlights: • The N-doped graphene oxide was confirmed with FTIR, EDS, HR-TEM, SAED, HRXPS, UV, TGA, DSC and CV. • The N-doped graphene oxide was found suitable for thermal and electrochemical applications. • The proposed mechanisms of thermal and electrochemical stabilities were experimentally verified. • The cyclic voltammetry N-doped graphene oxide implies its potential for manifold electrochemical applications. - Abstract: The kinetically active two dimensional surface of graphene oxide (GrO) plays an important role in understanding the chemistry of graphene. The GrO is comprises of carbon and oxygen while the f-(6-AIND) GrO contains nitrogen along with carbon and oxygen. The prominent thermal instability of GrO is widely explored. However, due to the synergistic impact of their constituting elements, the thermal and electrochemical stability of f-(6-AIND) GrO enhances after N-doping with nitrogen containing heterocycles like 6-Aminoindazole. Hence it is essential to probe the mutual impact of various functionalities present over the surface of GrO, to understand the mechanism of direct functionalization of GrO with thermal and electrochemical stabilities. Therefore, the decomposition kinetics of discrete atomic domains and their effect on thermal stability of f-(6-AIND) GrO was revealed with spectroscopic analysis and thermal assessment. Additionally, the mechanism of thermal transformation is precisely developed to demonstrate the impact of heat on weight loss due to the mass transfer. Likewise, the electrochemical properties can be well understood with the help of mechanism of electrochemical activity and cyclic voltammetry experiments. Also, the f-(6-AIND) GrO is confirmed with the help of various surface analysis techniques like FTIR, EDS, HR-XPS, HR-TEM, CV, SAED, TGA, DSC and UV-vis.

  8. The phylogenetic position of Anaplasma bovis, and inferences on the phylogeny of the genus Anaplasma.

    OpenAIRE

    YBAÑEZ, Adrian Patalinghug; Sashika, Mariko; Inokuma, Hisashi; 猪熊, 壽

    2014-01-01

    The present study aimed to determine the complete citrate synthase (gltA) and heat-shock protein (groEL) gene sequences of Anaplasma bovis and to infer phylogenetic relationships within the genus Anaplasma. Multiple alignments from single and concatenated sequences of the 16S rRNA, gltA and groEL genes of the genus Anaplasma were subjected to phylogenetic analyses. Percent identities of A. bovis nucleotide sequences were found highest with A. phagocytophilum in gltA (65.4%) and groEL (79.8%)....

  9. The Phylogenetic Position of Anaplasma bovis and Inferences on the Phylogeny of the Genus Anaplasma

    OpenAIRE

    YBAÑEZ, Adrian Patalinghug; Sashika, Mariko; Inokuma, Hisashi

    2013-01-01

    ABSTRACT The present study aimed to determine the complete citrate synthase (gltA) and heat-shock protein (groEL) gene sequences of Anaplasma bovis and to infer phylogenetic relationships within the genus Anaplasma. Multiple alignments from single and concatenated sequences of the 16S rRNA, gltA and groEL genes of the genus Anaplasma were subjected to phylogenetic analyses. Percent identities of A. bovis nucleotide sequences were found highest with A. phagocytophilum in gltA (65.4%) and groEL...

  10. Scented males and choosy females: does male odor influence female mate choice in the Mediterranean fruit fly?

    Science.gov (United States)

    Shelly, Todd E; Edu, James; Pahio, Elaine; Nishimoto, Jon

    2007-12-01

    The Mediterranean fruit fly, Ceratitis capitata (Wiedemann), displays a lek mating system characterized by a high level of female discrimination among potential mates. The basis of female choice is not understood, but recent studies indicate that male exposure to the aroma of certain plant structures or essential oils may increase mating success. In particular, exposure to the aroma of ginger root oil (GRO) enhances male mating frequency, and several sterile-male release programs against C. capitata have incorporated 'aromatherapy' (large-scale exposure of pre-release insects to GRO) to increase the effectiveness of control efforts. We investigated the mechanism underlying female preference for GRO-exposed males. Two sets of experiments were conducted. In the first, we monitored female attraction to (1) freshly killed flies, or (2) paper discs that contained hexane extracts from varying treatments. In these tests, females were sighted more often (1) near GRO-exposed than non-exposed males (even when the males were visually concealed) and (2) near extracts from GRO-exposed than non-exposed males. These findings suggest a 'perfume effect', whereby female mate choice is mediated by olfactory differences. In the second set, we compared (1) mate choice between intact females and females from which both antennae had been surgically removed, and (2) mating success between intact males and males from which both antennae had been surgically removed before GRO exposure. Intact females preferred GRO-exposed males, whereas females lacking both antennae rarely mated and showed no preference between GRO-exposed and non-exposed males. In the opposite treatment (intact females but surgically altered males), GRO-exposed males lacking both antennae mated as frequently as GRO-exposed intact males. These data suggest that female choice was dependent on olfactory perception of male odor but that male mating success did not depend on olfactory perception of GRO aroma, suggesting, in

  11. Characterization of Lactococcus lactis mutants with improved performance at high temperatures and potential dairy applications

    DEFF Research Database (Denmark)

    Chen, Jun

    (TM29) capable of growing at 40ºC was successfully isolated. By determining the temperature dependent growth rate profile, a shift of the optimum temperature from 30ºC to 36ºC was observed for TM29. Meanwhile, metabolic flux analysis revealed that TM29 was able to hold higher glucose consumption...... microarray analysis revealed apparent differences in the transcriptional response to heat between the mutant and parent. It was found that SNPs preceding gene groESL and ribU resulted in over-expression of chaperone proteins GroES-GroEL, and membrane associated riboflavin transporter protein RibU in TM29...

  12. Development of Solar Powered Feeding Scheme for Wireless Sensor Networks in low Solar Density Conditions / Bezvadu Sensoru Tīklu Elektroapgādes Sistēmas Izstrāde, Kas Izmanto Saules Paneļus Un Darbojas Pazeminātas Saules Radiācijas Apstākļos

    Science.gov (United States)

    Kondratjevs, K.; Zabasta, A.; Selmanovs-Pless, V.

    2015-08-01

    kontekstā ar reģionālajiem apstākļiem un aprēķināt darba režīmus bezvadu tīkla komponentēm vai pieņemt lēmumus par to funkcionalitātes pielāgošanu. Izstrādātais vadības modulis sastāv no saules paneļa fotoelementu moduļa, uzglabāšanas risinājuma (litija vai līdzvērtīgas baterijas) un elektroapgādes pārvaldības moduļa. Pētījuma novitāte ir elektroapgādes pārvaldības modulis, kas nodrošina stabilu un nepārtrauktu elektronisko iekārto darbību dažādos barošanas režīmos, dažādās situācijās, vienlaikus nodrošinot enerģijas saglabāšanu un moduļa sastāvdaļu ilgtspēju. Izstrādātais risinājums nodrošina nepārtrauktu 5V barošanu elektronikas shēmām bez strāvas pārtraukuma, kad notiek komutācija starp barošanas avotiem un enerģijas plūsmām dažādos virzienos. Elektroapgādes pārvaldības modulis nodrošina stabilu spriegumu mainīgos saules radiācijas apstākļos.

  13. The Aqueous Alteration of CR Chondrites: Experiments and Geochemical Modeling

    Science.gov (United States)

    Perronnet, M.; Berger, G.; Zolensky, M. E.; Toplis, M. J.; Kolb, V. M.; Bajagic, M.

    2007-03-01

    Laboratory alteration experiments were performed on mineralogical assemblages having the unaltered CR composition. The mineralogy of reaction products was compared to that of Renazzo and GRO 95577 and to predictions of geochemical modeling.

  14. CAPSULE REPORT - MANAGING CYANIDE IN METAL FINISHING

    Science.gov (United States)

    The purpose of this document is to provide guidance to surface finishing manufacturers, metal finishing decision maker and regulators on management practices and control technologies for managing cyanide in the workplace. This information can benefit key industry stakeholder gro...

  15. Yeast Interacting Proteins Database: YMR316W, YER125W [Yeast Interacting Proteins Database

    Lifescience Database Archive (English)

    Full Text Available YMR316W DIA1 Protein of unknown function, involved in invasive and pseudohyphal gro... of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion p

  16. Groundwater Management for Agriculture and Nature : an Economic Analysis

    NARCIS (Netherlands)

    Hellegers, P.

    2001-01-01

    Key words: desiccation of nature, economics of water management, groundwater extraction, groundwater level management, ecohydrology, agriculture, policy instruments.As a result of declining groundwater levels, nature in the Netherlands is suffering from desiccation. Since measures taken to raise gro

  17. 17α-Ethyl-5β-estrane-3α,17β-diol, a biological marker for the abuse of norethandrolone and ethylestrenol in slaughter cattle

    NARCIS (Netherlands)

    Puymbroeck, M. van; Kuilman, M.E.M.; Maas, R.F.M.; Witkamp, R.F.; Leyssens, L.; Miert, A.S.J.P.A.M. van; Hendriks, L.; Zande, D.J.M. van der; Adriaensens, P.; Jacobs, M.-P.; Raus, J.

    1999-01-01

    The metabolism of the illegal growth promoter ethylestrenol (EES) was evaluated in bovine liver cells and subcellular fractions of bovine liver preparations. Incubations with bovine microsomal preparations revealed that EES is extensively biotransformed into norethandrolone (NE), another illegal gro

  18. The United South and Eastern Tribe (USET) Proper Functioning Condition (PFC) and Tribal Focused Environmental Risk and Sustainability Tool (Tribal-FERST) Train the Trainers Workshop.October 6-9, 2014, in Nashville, Tennessee: PFC Assessment for Management and Monitoring

    Science.gov (United States)

    The maintenance of wildlife and aquatic habitat is dependent on the development of a riparian area management strategy, which considers and adapts to certain basic ecological and economic relationships. These relationships are functions of riparian and terrestrial ecosystems, gro...

  19. THE ROLE OF BIOTECH:'\\OLOGY IN CROP IMPROVEMENT ...

    African Journals Online (AJOL)

    BSN

    due to high population increase has lead to the grO\\\\ ing concern about loss in genetic diversity. under ... Biotechnology is indeed a frontier technology fast emerging from the laborator) to ...... projectile for delivering nucleic acid into living cell.

  20. Experiment list: SRX186644 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available stone deacetylase 1 (HDAC1) is a class I histone deacetylase that catalyzes the removal of the acetyl group ...C1) is a class I histone deacetylase that catalyzes the removal of the acetyl gro

  1. Experiment list: ERX402106 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available fection || ENA-CHECKLIST=ERC000011 http://dbarchive.bios...phila Kc167 and S2R+ cell lines organism=Drosophila melanogaster || cell line=Kc167 || genotype=gro RNA || genetic modification=trans

  2. Experiment list: ERX402124 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available sfection || ENA-CHECKLIST=ERC000011 http://dbarchive.bio...ophila Kc167 and S2R+ cell lines organism=Drosophila melanogaster || cell line=Kc167 || genotype=gro RNA || genetic modification=tran

  3. Experiment list: ERX402125 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available sfection || ENA-CHECKLIST=ERC000011 http://dbarchive.bio...ophila Kc167 and S2R+ cell lines organism=Drosophila melanogaster || cell line=Kc167 || genotype=gro RNA || genetic modification=tran

  4. Experiment list: ERX402107 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available sfection || ENA-CHECKLIST=ERC000011 http://dbarchive.bio...ophila Kc167 and S2R+ cell lines organism=Drosophila melanogaster || cell line=Kc167 || genotype=gro RNA || genetic modification=tran

  5. Experiment list: ERX402139 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ila Kc167 and S2R+ cell lines organism=Drosophila melanogaster || cell line=Kc167 || genotype=gro RNA || genetic modification=transfe...ction || ENA-CHECKLIST=ERC000011 http://dbarchive.biosci

  6. Effects of vasopressin and adrenaline on cerebral resuscitation in rats undergone cardiopulmonary resuscitation%血管加压素与肾上腺素对大鼠脑复苏效果的影响

    Institute of Scientific and Technical Information of China (English)

    杨伟伟; 彭鹏

    2011-01-01

    Objective To compare the effects of vasopressin and adrenaline on cerebral resuscitation in rats. Method Sixty male SD rats were used to set the animal model of cardiac arrest and were randomly (random number) divided into 4 groups (n = 15/group): sham operation group (sham-gro), negative control group (neg-gro), vasopressin group (vas-gro) and adrenaline group (adr-gro). Blood pressure was recorded, and brain tissue samples were obtained. Results There was no significant difference in the recovery rate between vas-gro and adr-gro 30 min after resuscitation (P <0.05). The mean blood pressure in vasgro was higher than that in adr-gro within 30 min (1,2, 5, 10, 20, 30 min) after restoration of spontaneous circulation. The measured MDA, SOD and GSH were significantly different (P < 0.05) between each two groups among vas-gro, sham-gro and adr-gro. Conclusions The recovery rate was similar between vasopressin group and adrenaline group during cardiopulmonary resuscitation in rats. However, vasopressin can to maintain the mean arterial pressure at a higher level after resuscitation, which can the increase the cerebral perfusion and reduce brain cell damage.%目的 比较血管加压素与肾上腺素对大鼠脑复苏效果的研究.方法 60只雄性SD大鼠建立心搏骤停模型,随机(随机数字法)分为4组(n=15/组):假手术组(sham operated group,shamgro)、阴性对照组(neg-gro)、血管加压素组(vas-gro)、肾上腺素组(adr-gro).分别记录血压变化,并取大鼠脑组织标本.结果 在复苏30 min后vas-gro与adr-gro复苏成功率差异无统计学意义(P>0.05);在自主循环恢复的30 min内(1,2,5,10,20,30 min)vas-gro的平均动脉压均高于adr-gro;测得MDA,SOD,GSH的量vas-gro,sham-gro,adr-gro互相之间差异有统计学意义(P<0.05).结论 血管加压素与肾上腺素在大鼠心肺复苏过程中成功率相近,但血管加压素能维持复苏后平均动脉压在较高水平,继而会更好的增加脑灌注,减轻脑细胞损伤.

  7. Feste - Opern - Prozessionen, hrg. von Katharina Hottmann und Christine Siegert, Hildesheim: Olms 2008

    National Research Council Canada - National Science Library

    Sarah Manthey

    2010-01-01

    ...)Theaterwissenschaft und der Performance-Forschung einiger Popularität erfreut, und so wird auch von den Herausgeberinnen Katharina Hottmann und Christine Siegert interdisziplinäre Öffnung groß geschrieben...

  8. Health for all or riches for some: WHO's responsible?

    Science.gov (United States)

    Motchane, Jean-Loup

    2003-01-01

    Can we still rely on the World Health Organization? It has not openly opposed the greed of the major global pharmaceutical companies, and its director-general, Gro Harlem Brundtland, has deferred to them.

  9. Effects of Temperature, Salinity and Seed Age on Induction of Zostera japonica Germination in North America, USA

    Science.gov (United States)

    Seagrasses can colonize unstructured mudflats either through clonal growth or seed germination and survival. Zostera japonica is an introduced seagrass in North America that has rapidly colonized mudflats along the Pacific Coast, leading to active management of the species. Gro...

  10. Price-predicting ability of farm managers : empirical findings with flower producers in the Netherlands

    NARCIS (Netherlands)

    Trip, G.; Huirne, R.B.M.; Renkema, J.A.

    2000-01-01

    Differences in income among horticultural growers producing under similar conditions are known to be substantial. Production policy, including cultivar choice, plays an important role. Both price variation over time and price differences among cultivars provide valuable management information to gro

  11. Ethnic Group Identification and Group Evaluation Among Minority and Majority Groups : Testing the Multiculturalism Hypothesis

    NARCIS (Netherlands)

    Verkuyten, Maykel J. A. M.

    2005-01-01

    Following social identity theory, the author hypothesized that members of minority groups are more likely than majority group members to endorse multiculturalism more strongly and assimilationist thinking less strongly. In addition, the multiculturalism hypothesis proposes that the more minority gro

  12. Dicty_cDB: Contig-U04916-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 034718 ) Gracilaria robusta plasmid Gro4970, complete sequ... 44 5.9 1 ( DL064443 ) Cancer Gene Determination... and Therapeutic Screeni... 44 5.9 1 ( DL064206 ) Cancer Gene Determination and Therapeutic Screeni... 44 5

  13. Chaperonins fight aminoglycoside-induced protein misfolding and promote short-term tolerance in Escherichia coli

    DEFF Research Database (Denmark)

    Goltermann, Lise; Good, Liam; Bentin, Thomas

    2013-01-01

    For almost half of a century, we have known that aminoglycoside antibiotics corrupt ribosomes, causing translational misreading, yet it remains unclear whether or not misreading triggers protein misfolding, and possible effects of chaperone action on drug susceptibilities are poorly understood....... Here, we show that aminoglycosides cause cytosolic protein misfolding and that chaperonin GroEL/GroES overexpression counters this defect. During aminoglycoside exposure to exponential cultures, chaperonin overexpression protected the bacterial membrane potential, rescued cell growth, and facilitated...... as measured by reduced minimum inhibitory concentrations, whereas GroEL/GroES overexpression did not increase minimum inhibitory concentrations. Our observations establish misfolding of cytosolic proteins as an effect of aminoglycoside action and reveal that chaperones, chaperonins in particular, help...

  14. Effects of Temperature, Salinity and Seed Age on Induction of Zostera japonica Germination in North America, USA

    Science.gov (United States)

    Seagrasses can colonize unstructured mudflats either through clonal growth or seed germination and survival. Zostera japonica is an introduced seagrass in North America that has rapidly colonized mudflats along the Pacific Coast, leading to active management of the species. Gro...

  15. The United South and Eastern Tribe (USET) Proper Functioning Condition (PFC) and Tribal Focused Environmental Risk and Sustainability Tool (Tribal-FERST) Train the Trainers Workshop.October 6-9, 2014, in Nashville, Tennessee: PFC Assessment for Management and Monitoring

    Science.gov (United States)

    The maintenance of wildlife and aquatic habitat is dependent on the development of a riparian area management strategy, which considers and adapts to certain basic ecological and economic relationships. These relationships are functions of riparian and terrestrial ecosystems, gro...

  16. Development of iron and zinc enriched mungbean (Vigna radiata L.) cultivars with agronomic traits in consideration

    NARCIS (Netherlands)

    Singh, R.

    2013-01-01

    Malnutrition in India, particularly among women, children and adolescents is an emergency that needs immediate attention in this fast growing and developing country. Micronutrient deficiencies are threatening public health in India more and more. Deficiencies of micronutrients drastically affect gro

  17. Wutz Handbuch Vakuumtechnik

    CERN Document Server

    2012-01-01

    Dieses Standardwerk gibt dem Leser umfassend Auskunft über Theorie und Praxis der Vakuumtechnik. Eine große Anzahl von numerischen Beispielen sowie aussagekräftigen Abbildungen erläutert und visualisiert die theoretischen Sachverhalte.

  18. Inhibitory Effects of Cytosolic Ca2+ Concentration by Ginsenoside Ro Are Dependent on Phosphorylation of IP3RI and Dephosphorylation of ERK in Human Platelets

    Directory of Open Access Journals (Sweden)

    Hyuk-Woo Kwon

    2015-01-01

    Full Text Available Intracellular Ca2+ ([Ca2+]i is platelet aggregation-inducing molecule and is involved in activation of aggregation associated molecules. This study was carried out to understand the Ca2+-antagonistic effect of ginsenoside Ro (G-Ro, an oleanane-type saponin in Panax ginseng. G-Ro, without affecting leakage of lactate dehydrogenase, dose-dependently inhibited thrombin-induced platelet aggregation, and the half maximal inhibitory concentration was approximately 155 μM. G-Ro inhibited strongly thrombin-elevated [Ca2+]i, which was strongly increased by A-kinase inhibitor Rp-8-Br-cAMPS compared to G-kinase inhibitor Rp-8-Br-cGMPS. G-Ro increased the level of cAMP and subsequently elevated the phosphorylation of inositol 1, 4, 5-triphosphate receptor I (IP3RI (Ser1756 to inhibit [Ca2+]i mobilization in thrombin-induced platelet aggregation. Phosphorylation of IP3RI (Ser1756 by G-Ro was decreased by PKA inhibitor Rp-8-Br-cAMPS. In addition, G-Ro inhibited thrombin-induced phosphorylation of ERK 2 (42 kDa, indicating inhibition of Ca2+ influx across plasma membrane. We demonstrate that G-Ro upregulates cAMP-dependent IP3RI (Ser1756 phosphorylation and downregulates phosphorylation of ERK 2 (42 kDa to decrease thrombin-elevated [Ca2+]i, which contributes to inhibition of ATP and serotonin release, and p-selectin expression. These results indicate that G-Ro in Panax ginseng is a beneficial novel Ca2+-antagonistic compound and may prevent platelet aggregation-mediated thrombotic disease.

  19. Molekulargenetische Optimierung der Sphingoidbasen-Produktion der nicht-konventionellen Hefe Pichia ciferrii

    OpenAIRE

    Schorsch, Christoph

    2012-01-01

    Die nicht-konventionelle Hefe P. ciferrii produziert große Mengen der tetra-acetylierten Sphingoidbase Phytosphingosin (TAPS). Sphingoidbasen sind essentielle Komponenten des stratum corneums, der multilamellaren Barriere der menschlichen Haut, und daher in der Kosmetik-Industrie von großem Interesse. Im Rahmen dieser Arbeit sollte die biotechnologische Produktion der Sphingoidbasen Phytosphingosin, Sphinganin und Sphingosin auf molekularbiologischer Ebene in P. ciferrii charakterisiert und o...

  20. Heterologous expression, chaperone mediated solubilization and purification of parasitic nematode-specific growth factor-like protein of Setaria digitata

    Institute of Scientific and Technical Information of China (English)

    W WP Rodrigo; R S Dassanayake; E H Karunanayake; YIN Silva Gunawardene; O VDS J Weerasena

    2014-01-01

    Objective:To clone, express and purify a putative parasitic nematode specific protein of Setaria digitata (S. digitata), filarial nematode that infects livestock and cause significant economic losses inFarEast andAsia to be used for structural and functional analyses. Methods:To characterize uncharacterized gene ofS. digitata(SDUG), the herterologous expression ofSDUG was carried out in the pET [cloned into pET45b(+)] expression system initially and co-expression ofSDUG using chaperone plasmids pG-KJE8, pGro7, pKJE7, pG-Tf2 and pTf16 containing chaperone proteins of dnaK-dnaJ-grpE-groES-gro-E, groES-groEL, dnaK-dnaJ-grpE, groES-groEL-tig, and tig respectively, was carried out subsequently.Results:Expression ofSDUG was seen whenEscherichia coli strainBL21(DE3) is used, while concentrating protein largely into the insoluble fraction.The co-expression ofSDUG using chaperone plasmid mediated system indicated a significant increase of the protein in the soluble fraction.Of the chaperon plasmid sets, the highest amount of recombinantSDUP in the soluble fraction was seen when pGro7 was used in the presence of 2 mg/mLL-arabinose and0.6MIPTG concentration in the culture medium and for3 h of incubation at the temperature of28 ℃.RecombinantSDUG was purified both from soluble and insoluble fractions usingNi affinity chromatography.SDS-PAGE and western blot analyses of these proteins revealed a single band having expected size of ~24 kDa.Conclusions:SDUG seems to be more aggregate-prone and hydrophobic in nature and such protein can make soluble by correct selecting the inducer concentrations and induction temperature and its duration.

  1. Tissue MicroArray (TMA) analysis of normal and persistent Chlamydophila pneumoniae infection.

    Science.gov (United States)

    Borel, Nicole; Mukhopadhyay, Sanghamitra; Kaiser, Carmen; Sullivan, Erin D; Miller, Richard D; Timms, Peter; Summersgill, James T; Ramirez, Julio A; Pospischil, Andreas

    2006-10-19

    Chlamydophila pneumoniae infection has been implicated as a potential risk factor for atherosclerosis, however the mechanism leading to persistent infection and its role in the disease process remains to be elucidated. We validated the use of tissue microarray (TMA) technology, in combination with immunohistochemistry (IHC), to test antibodies (GroEL, GroES, GspD, Ndk and Pyk) raised against differentially expressed proteins under an interferon-gamma (IFN-gamma) induced model of chlamydial persistence. In the cell pellet array, we were able to identify differences in protein expression patterns between untreated and IFN-gamma treated samples. Typical, large chlamydial inclusions could be observed in the untreated samples with all antibodies, whereas the number of inclusions were decreased and were smaller and atypical in shape in the IFN-gamma treated samples. The staining results obtained with the TMA method were generally similar to the changes observed between normal and IFN-gamma persistence using proteomic analysis. Subsequently, it was shown in a second TMA including archival atheromatous heart tissues from 12 patients undergoing heart transplantation, that GroEL, GroES, GspD and Pyk were expressed in atheromatous heart tissue specimens as well, and were detectable morphologically within lesions by IHC. TMA technology proved useful in documenting functional proteomics data with the morphologic distribution of GroEL, GroES, GspD, Ndk and Pyk within formalin-fixed, paraffin-embedded cell pellets and tissues from patients with severe coronary atherosclerosis. The antibodies GroEL and GroES, which were upregulated under persistence in proteomic analysis, displayed positive reaction in atheromatous heart tissue from 10 out of 12 patients. These may be useful markers for the detection of persistent infection in vitro and in vivo.

  2. Tissue MicroArray (TMA analysis of normal and persistent Chlamydophila pneumoniae infection

    Directory of Open Access Journals (Sweden)

    Timms Peter

    2006-10-01

    Full Text Available Abstract Background Chlamydophila pneumoniae infection has been implicated as a potential risk factor for atherosclerosis, however the mechanism leading to persistent infection and its role in the disease process remains to be elucidated. Methods We validated the use of tissue microarray (TMA technology, in combination with immunohistochemistry (IHC, to test antibodies (GroEL, GroES, GspD, Ndk and Pyk raised against differentially expressed proteins under an interferon-gamma (IFN-γ induced model of chlamydial persistence. Results In the cell pellet array, we were able to identify differences in protein expression patterns between untreated and IFN-γ treated samples. Typical, large chlamydial inclusions could be observed in the untreated samples with all antibodies, whereas the number of inclusions were decreased and were smaller and atypical in shape in the IFN-γ treated samples. The staining results obtained with the TMA method were generally similar to the changes observed between normal and IFN-γ persistence using proteomic analysis. Subsequently, it was shown in a second TMA including archival atheromatous heart tissues from 12 patients undergoing heart transplantation, that GroEL, GroES, GspD and Pyk were expressed in atheromatous heart tissue specimens as well, and were detectable morphologically within lesions by IHC. Conclusion TMA technology proved useful in documenting functional proteomics data with the morphologic distribution of GroEL, GroES, GspD, Ndk and Pyk within formalin-fixed, paraffin-embedded cell pellets and tissues from patients with severe coronary atherosclerosis. The antibodies GroEL and GroES, which were upregulated under persistence in proteomic analysis, displayed positive reaction in atheromatous heart tissue from 10 out of 12 patients. These may be useful markers for the detection of persistent infection in vitro and in vivo.

  3. Quasistatic and Dynamic Growth of Microscale Spherical Voids (Preprint)

    Science.gov (United States)

    2008-01-01

    function of gro\\ VIh rate. Eq. (56) is solved numerically for specified material data. Results of crossover growlh rate, v·, and pressure, P’, are shown...At a pressure P < p’, (or v < v"), growth rate is lower for materials with high yield stress. Conversely. at a pressure P > p •. (or v > v·), gro\\ VIh

  4. [Host factors in the regulation of the Vibrio fischeri lux operon in Escherichia coli cells].

    Science.gov (United States)

    Manukhov, I V; Kotova, V Iu; Zavil'gel'skiĭ, G B

    2006-01-01

    It has been shown that the chaperonin GroEL, together with GroES co-chaperonin and Lon ATP-dependent protease are involved in the regulation of expression of the Vibrio fischeri lux operon in Escherichia coli cells. The cells of E. coli groE (pF1)- bearing a plasmid with the complete V. fischeri lux regulon were weakly luminescent. The cells of E. coli lonA (pF1) displayed intense bioluminescence. The same effects also occurred in mutant E. coli strains bearing a hybrid plasmid pVFR1, where the luxR gene and the regulatory region of the V. fischeri lux operon were inserted before the Photorhabdus luminescens luxCDABE cassette. The V. fischeri luxR gene was cloned in the pGEX-KG vector with the formation of a hybrid gene gst-luxR. It was shown that affinity chromatography of the product of expression, the chimeric protein GST-LuxR, on a column with glutathione-agarose resulted in its copurification with the proteins GroEL and Lon. Consequently, LuxR, the transcription activator of the lux operon, forms complexes with these proteins. It is supposed that GroEL/GroES is responsible for the folding of the LuxR protein, and Lon protease degrades the LuxR protein either before its folding into an active globule or at denaturing.

  5. Non-structural proteins P17 and P33 are involved in the assembly of the internal membrane-containing virus PRD1

    Energy Technology Data Exchange (ETDEWEB)

    Karttunen, Jenni; Mäntynen, Sari [Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science and Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014 Jyväskylä (Finland); Ihalainen, Teemu O. [Stem Cells in Neurological Applications Group, BioMediTech, University of Tampere, Tampere (Finland); Bamford, Jaana K.H. [Centre of Excellence in Biological Interactions, Department of Biological and Environmental Science and Nanoscience Center, University of Jyväskylä, P.O. Box 35, 40014 Jyväskylä (Finland); Oksanen, Hanna M., E-mail: hanna.oksanen@helsinki.fi [Institute of Biotechnology and Department of Biosciences, University of Helsinki, Biocenter 2, P.O. Box 56 (Viikinkaari 5), FIN-00014 Helsinki (Finland)

    2015-08-15

    Bacteriophage PRD1, which has been studied intensively at the structural and functional levels, still has some gene products with unknown functions and certain aspects of the PRD1 assembly process have remained unsolved. In this study, we demonstrate that the phage-encoded non-structural proteins P17 and P33, either individually or together, complement the defect in a temperature-sensitive GroES mutant of Escherichia coli for host growth and PRD1 propagation. Confocal microscopy of fluorescent fusion proteins revealed co-localisation between P33 and P17 as well as between P33 and the host chaperonin GroEL. A fluorescence recovery after photobleaching assay demonstrated that the diffusion of the P33 fluorescent fusion protein was substantially slower in E. coli than theoretically calculated, presumably resulting from intermolecular interactions. Our results indicate that P33 and P17 function in procapsid assembly, possibly in association with the host chaperonin complex GroEL/GroES. - Highlights: • Two non-structural proteins of PRD1 are involved in the virus assembly. • P17 and P33 complement the defect in GroES of Escherichia coli. • P33 co-localises with GroEL and P17 in the bacterium. • Slow motion of P33 in the bacterium suggests association with cellular components.

  6. Comparison of Oncentra® Brachy IPSA and graphical optimisation techniques: a case study of HDR brachytherapy head and neck and prostate plans

    Energy Technology Data Exchange (ETDEWEB)

    Jameson, Michael G, E-mail: michael.jameson@sswahs.nsw.gov.au [Liverpool and Macarthur Cancer Therapy Centres, Liverpool, New South Wales (Australia); Centre for Medical Radiation Physics, University of Wollongong, Wollongong, New South Wales (Australia); Ingham Institute of Applied Medical Research, Liverpool, New South Wales (Australia); Ohanessian, Lucy [Liverpool and Macarthur Cancer Therapy Centres, Liverpool, New South Wales (Australia); Batumalai, Vikneswary [Liverpool and Macarthur Cancer Therapy Centres, Liverpool, New South Wales (Australia); Ingham Institute of Applied Medical Research, Liverpool, New South Wales (Australia); South Western Sydney Clinical School, School of Medicine, University of New South Wales (Australia); Patel, Virendra [Liverpool and Macarthur Cancer Therapy Centres, Liverpool, New South Wales (Australia); Holloway, Lois C [Liverpool and Macarthur Cancer Therapy Centres, Liverpool, New South Wales (Australia); Centre for Medical Radiation Physics, University of Wollongong, Wollongong, New South Wales (Australia); Ingham Institute of Applied Medical Research, Liverpool, New South Wales (Australia); South Western Sydney Clinical School, School of Medicine, University of New South Wales (Australia); Institute of Medical Physics, School of Physics, University of Sydney, Sydney, New South Wales (Australia)

    2015-06-15

    There are a number of different dwell positions and time optimisation options available in the Oncentra® Brachy (Elekta Brachytherapy Solutions, Veenendaal, The Netherlands) brachytherapy treatment planning system. The purpose of this case study was to compare graphical (GRO) and inverse planning by simulated annealing (IPSA) optimisation techniques for interstitial head and neck (HN) and prostate plans considering dosimetry, modelled radiobiology outcome and planning time. Four retrospective brachytherapy patients were chosen for this study, two recurrent HN and two prostatic boosts. Manual GRO and IPSA plans were generated for each patient. Plans were compared using dose–volume histograms (DVH) and dose coverage metrics including; conformity index (CI), homogeneity index (HI) and conformity number (CN). Logit and relative seriality models were used to calculate tumour control probability (TCP) and normal tissue complication probability (NTCP). Approximate planning time was also recorded. There was no significant difference between GRO and IPSA in terms of dose metrics with mean CI of 1.30 and 1.57 (P > 0.05) respectively. IPSA achieved an average HN TCP of 0.32 versus 0.12 for GRO while for prostate there was no significant difference. Mean GRO planning times were greater than 75 min while average IPSA planning times were less than 10 min. Planning times for IPSA were greatly reduced compared to GRO and plans were dosimetrically similar. For this reason, IPSA makes for a useful planning tool in HN and prostate brachytherapy.

  7. Extending the evaluation of Genia Event task toward knowledge base construction and comparison to Gene Regulation Ontology task.

    Science.gov (United States)

    Kim, Jin-Dong; Kim, Jung-Jae; Han, Xu; Rebholz-Schuhmann, Dietrich

    2015-01-01

    The third edition of the BioNLP Shared Task was held with the grand theme "knowledge base construction (KB)". The Genia Event (GE) task was re-designed and implemented in light of this theme. For its final report, the participating systems were evaluated from a perspective of annotation. To further explore the grand theme, we extended the evaluation from a perspective of KB construction. Also, the Gene Regulation Ontology (GRO) task was newly introduced in the third edition. The final evaluation of the participating systems resulted in relatively low performance. The reason was attributed to the large size and complex semantic representation of the ontology. To investigate potential benefits of resource exchange between the presumably similar tasks, we measured the overlap between the datasets of the two tasks, and tested whether the dataset for one task can be used to enhance performance on the other. We report an extended evaluation on all the participating systems in the GE task, incoporating a KB perspective. For the evaluation, the final submission of each participant was converted to RDF statements, and evaluated using 8 queries that were formulated in SPARQL. The results suggest that the evaluation may be concluded differently between the two different perspectives, annotation vs. KB. We also provide a comparison of the GE and GRO tasks by converting their datasets into each other's format. More than 90% of the GE data could be converted into the GRO task format, while only half of the GRO data could be mapped to the GE task format. The imbalance in conversion indicates that the GRO is a comprehensive extension of the GE task ontology. We further used the converted GRO data as additional training data for the GE task, which helped improve GE task participant system performance. However, the converted GE data did not help GRO task participants, due to overfitting and the ontology gap.

  8. A comparison of inverse optimization algorithms for HDR/PDR prostate brachytherapy treatment planning.

    Science.gov (United States)

    Dinkla, Anna M; van der Laarse, Rob; Kaljouw, Emmie; Pieters, Bradley R; Koedooder, Kees; van Wieringen, Niek; Bel, Arjan

    2015-01-01

    Graphical optimization (GrO) is a common method for high-dose-rate/pulsed-dose-rate (PDR) prostate brachytherapy treatment planning. New methods performing inverse optimization of the dose distribution have been developed over the past years. The purpose is to compare GrO and two established inverse methods, inverse planning simulated annealing (IPSA) and hybrid inverse treatment planning and optimization (HIPO), and one new method, enhanced geometric optimization-interactive inverse planning (EGO-IIP), in terms of speed and dose-volume histogram (DVH) parameters. For 26 prostate cancer patients treated with a PDR brachytherapy boost, an experienced treatment planner optimized the dose distributions using four different methods: GrO, IPSA, HIPO, and EGO-IIP. Relevant DVH parameters (prostate-V100%, D90%, V150%; urethra-D(0.1cm3) and D(1.0cm3); rectum-D(0.1cm3) and D(2.0cm3); bladder-D(2.0cm3)) were evaluated and their compliance to the constraints. Treatment planning time was also recorded. All inverse methods resulted in shorter planning time (mean, 4-6.7 min), as compared with GrO (mean, 7.6 min). In terms of DVH parameters, none of the inverse methods outperformed the others. However, all inverse methods improved on compliance to the planning constraints as compared with GrO. On average, EGO-IIP and GrO resulted in highest D90%, and the IPSA plans resulted in lowest bladder D2.0cm3 and urethra D(1.0cm3). Inverse planning methods decrease planning time as compared with GrO for PDR/high-dose-rate prostate brachytherapy. DVH parameters are comparable for all methods. Copyright © 2015 American Brachytherapy Society. Published by Elsevier Inc. All rights reserved.

  9. Identification of a mammalian glycerol-3-phosphate phosphatase: Role in metabolism and signaling in pancreatic β-cells and hepatocytes.

    Science.gov (United States)

    Mugabo, Yves; Zhao, Shangang; Seifried, Annegrit; Gezzar, Sari; Al-Mass, Anfal; Zhang, Dongwei; Lamontagne, Julien; Attane, Camille; Poursharifi, Pegah; Iglesias, José; Joly, Erik; Peyot, Marie-Line; Gohla, Antje; Madiraju, S R Murthy; Prentki, Marc

    2016-01-26

    Obesity, and the associated disturbed glycerolipid/fatty acid (GL/FA) cycle, contribute to insulin resistance, islet β-cell failure, and type 2 diabetes. Flux through the GL/FA cycle is regulated by the availability of glycerol-3-phosphate (Gro3P) and fatty acyl-CoA. We describe here a mammalian Gro3P phosphatase (G3PP), which was not known to exist in mammalian cells, that can directly hydrolyze Gro3P to glycerol. We identified that mammalian phosphoglycolate phosphatase, with an uncertain function, acts in fact as a G3PP. We found that G3PP, by controlling Gro3P levels, regulates glycolysis and glucose oxidation, cellular redox and ATP production, gluconeogenesis, glycerolipid synthesis, and fatty acid oxidation in pancreatic islet β-cells and hepatocytes, and that glucose stimulated insulin secretion and the response to metabolic stress, e.g., glucolipotoxicity, in β-cells. In vivo overexpression of G3PP in rat liver lowers body weight gain and hepatic glucose production from glycerol and elevates plasma HDL levels. G3PP is expressed at various levels in different tissues, and its expression varies according to the nutritional state in some tissues. As Gro3P lies at the crossroads of glucose, lipid, and energy metabolism, control of its availability by G3PP adds a key level of metabolic regulation in mammalian cells, and G3PP offers a potential target for type 2 diabetes and cardiometabolic disorders.

  10. Translocation boost protein-folding efficiency of double-barreled chaperonins.

    Science.gov (United States)

    Coluzza, Ivan; van der Vies, Saskia M; Frenkel, Daan

    2006-05-15

    Incorrect folding of proteins in living cells may lead to malfunctioning of the cell machinery. To prevent such cellular disasters from happening, all cells contain molecular chaperones that assist nonnative proteins in folding into the correct native structure. One of the most studied chaperone complexes is the GroEL-GroES complex. The GroEL part has a "double-barrel" structure, which consists of two cylindrical chambers joined at the bottom in a symmetrical fashion. The hydrophobic rim of one of the GroEL chambers captures nonnative proteins. The GroES part acts as a lid that temporarily closes the filled chamber during the folding process. Several capture-folding-release cycles are required before the nonnative protein reaches its native state. Here we report molecular simulations that suggest that translocation of the nonnative protein through the equatorial plane of the complex boosts the efficiency of the chaperonin action. If the target protein is correctly folded after translocation, it is released. However, if it is still nonnative, it is likely to remain trapped in the second chamber, which then closes to start a reverse translocation process. This shuttling back and forth continues until the protein is correctly folded. Our model provides a natural explanation for the prevalence of double-barreled chaperonins. Moreover, we argue that internal folding is both more efficient and safer than a scenario where partially refolded proteins escape from the complex before being recaptured.

  11. Eesti Post sokrashtshajet ljudei radi povõshenija effektivnosti / Jüri Ehasalu ; interv. Dmitri Zvjagintsev

    Index Scriptorium Estoniae

    Ehasalu, Jüri, 1953-

    2003-01-01

    Eesti Posti nõukogu esimees vastab küsimustele, mis puudutavad töötajate koondamist Eesti Postis, ettevõtte erastamise mõttekust, hinnangut olukorrale postiteenuste turul, uute postioperaatorite tuleku võimalust Eesti liitumisel EL-iga

  12. Chaperonin function depends on structure and disorder in co-chaperonin mobile loops.

    Science.gov (United States)

    Landry, S J; Steede, N K; Garaudy, A M; Maskos, K; Viitanen, P V

    1999-01-01

    Co-chaperonins from diverse organisms exhibit mobile loops which fold into a beta hairpin conformation upon binding to the chaperonin. GroES, Gp31, and human Hsp10 mobile loops exhibit a preference for the beta hairpin conformation in the free co-chaperonins, and the conformational dynamics of the human Hsp10 mobile loop appear to be restricted by nascent hairpin formation. Backbone conformational entropy must weigh against binding of co-chaperonins to chaperonins, and thus the conformational preferences of the loops may strongly influence chaperonin-binding affinity. Indeed, subtle mutations in the loops change GroEL-binding affinity and cause defects in chaperonin function, and these defects can be suppressed by mutations in GroEL which compensate for the changes in affinity. The fact that high-affinity co-chaperonin binding impairs chaperonin function has implications for the mechanism of chaperonin-assisted protein folding.

  13. The phylogenetic position of Anaplasma bovis and inferences on the phylogeny of the genus Anaplasma.

    Science.gov (United States)

    Ybañez, Adrian Patalinghug; Sashika, Mariko; Inokuma, Hisashi

    2014-03-01

    The present study aimed to determine the complete citrate synthase (gltA) and heat-shock protein (groEL) gene sequences of Anaplasma bovis and to infer phylogenetic relationships within the genus Anaplasma. Multiple alignments from single and concatenated sequences of the 16S rRNA, gltA and groEL genes of the genus Anaplasma were subjected to phylogenetic analyses. Percent identities of A. bovis nucleotide sequences were found highest with A. phagocytophilum in gltA (65.4%) and groEL (79.8%). Single gene phylogenetic tree results assumed similar phylogenetic positions within the genus Anaplasma, except for A. bovis. However, consensus and concatenated sequence phylogenetic trees showed similar results, revealing 2 subgroups within the genus.

  14. Instaliacija "Nusipuošimas"

    OpenAIRE

    Pociūtė, Gintarė

    2013-01-01

    Visuomenėje vyraujantys grožio standartai virsta mėginimu tiražuoti moterų išorę. Mane supa grožis, mano šeimos moterys taip auklėtos, joms tai svarbu. Svarbu ir man, nes siekiu pripažinimo ir slepiuosi. Visada slėpdavausi, užsidengdama kitų žmonių istorijomis, išgyvendama panašius jausmus. Atėjo laikas save apnuoginti. Plaukai man - vienas pagrindinių moteriškumo simbolių ir akcentų. Netekusi jų, netenku dalies savęs. Veidas tampa neatpažįstamas. Tai istorija ne apie ligą, tačiau grožio sie...

  15. Agonists and inverse agonists for the herpesvirus 8-encoded constitutively active seven-transmembrane oncogene product, ORF-74

    DEFF Research Database (Denmark)

    Rosenkilde, M M; Kledal, T N; Bräuner-Osborne, Hans

    1999-01-01

    A number of CXC chemokines competed with similar, nanomolar affinity against 125I-interleukin-8 (IL-8) binding to ORF-74, a constitutively active seven-transmembrane receptor encoded by human herpesvirus 8. However, in competition against 125I-labeled growth-related oncogene (GRO)-alpha, the ORF-74...... receptor was highly selective for GRO peptides, with IL-8 being 10,000-fold less potent. The constitutive stimulating activity of ORF-74 on phosphatidylinositol turnover was not influenced by, for example, IL-8 binding. In contrast, GRO peptides acted as potent agonists in stimulating ORF-74 signaling......, whereas IP-10 and stromal cell-derived factor-1alpha surprisingly acted as inverse agonists. These peptides had similar pharmacological properties with regard to enhancing or inhibiting, respectively, the stimulatory effect of ORF-74 on NIH-3T3 cell proliferation. Construction of a high affinity zinc...

  16. Expression, modulation and signalling of IL-17 receptor in fibroblast-like synoviocytes of patients with rheumatoid arthritis.

    Science.gov (United States)

    Kehlen, A; Thiele, K; Riemann, D; Langner, J

    2002-03-01

    Interleukin-17 (IL-17) has been characterized as a proinflammatory cytokine produced by CD4+ CD45RO+ memory T cells. Overproduction of IL-17 was detected in the synovium of patients with rheumatoid arthritis (RA) compared to patients with osteoarthritis. In contrast to the restricted expression of IL-17, the IL-17 receptor (IL-17R/CDw217) is expressed ubiquitously. Using a real-time RT-PCR assay, we detected similar absolute levels of IL-17R mRNA expression in fibroblast-like synoviocytes (SFC) from patients with RA (mean 9 pg/microg total RNA; ranged from 0.1 pg to 96 pg IL-17R mRNA/microg total RNA) compared to synoviocytes of non-RA patients. Analysis of the IL-17R surface expression confirmed the results obtained for IL-17R mRNA expression. Exposure of SFC to IL-17 led to a mRNA induction of CXC chemokines IL-8, GRO-alpha and GRO-beta. An anti-IL-17 antibody blocked these effects of IL-17. The MAPK p38 appears necessary for the regulation of IL-8, GRO-alpha and GRO-beta expression as shown by inhibition with SB203580. The inhibitors genistein (tyrosine kinase inhibitor) and calphostin C (inhibitor of protein kinase C) reduced significantly the IL-17-stimulated mRNA expression of IL-8, GRO-alpha and GRO-beta in SFC, whereas PD98059 (inhibitor of MEK-1/2) was without effect. Pharmacological drugs used in therapy of RA, such as cyclosporin and methotrexate, induced a fourfold increase of IL-17R mRNA expression and augmented the IL-17-stimulated IL-8 expression. Our results support the hypothesis that IL-17/IL-17R may play a significant role in the pathogenesis of RA contributing to an unbalanced production of cytokines as well as participating in connective tissue remodelling.

  17. Improved 1, 2, 4-butanetriol production from an engineered Escherichia coli by co-expression of different chaperone proteins.

    Science.gov (United States)

    Lu, Xinyao; He, Shuying; Zong, Hong; Song, Jian; Chen, Wen; Zhuge, Bin

    2016-09-01

    1, 2, 4-Butanetriol (BT) is a high-value non-natural chemical and has important applications in polymers, medical production and military industry. In the constructed BT biosynthesis pathway from xylose in Escherichia coli, the xylose dehydrogenase (Xdh) and the benzoylformate decarboxylase (MdlC) are heterologous enzymes and the activity of MdlC is the key limiting factor for BT production. In this study, six chaperone protein systems were introduced into the engineered E. coli harboring the recombinant BT pathway. The chaperone GroES-GroEL was beneficial to Xdh activity but had a negative effect on MdlC activity and BT titer. The plasmid pTf16 containing the tig gene (trigger factor) was beneficial to Xdh and MdlC activities and improved the BT titer from 0.42 to 0.56 g/l from 20 g/l xylose. However, co-expression of trigger factor and GroES-GroEL simultaneously reduced the activity of MdlC and had no effect on the BT production. The plasmid pKJE7 harboring dnaK-dnaJ-grpE showed significant negative effects on these enzyme activities and cell growth, leading to completely restrained the BT production. Similarly, co-expression of DnaKJ-GrpPE and GroES-GroEL simultaneously reduced Xdh and MdlC activities and decreased the BT titer by 45.2 %. The BT production of the engineered E. coli harboring pTf16 was further improved to the highest level at 1.01 g/l under pH control (pH 7). This work showed the potential application of chaperone proteins in microorganism engineering to get high production of target compounds as an effective and valuable tool.

  18. Developing principles of sustainability and stakeholder engagement for "gentle" remediation approaches: the European context.

    Science.gov (United States)

    Cundy, A B; Bardos, R P; Church, A; Puschenreiter, M; Friesl-Hanl, W; Müller, I; Neu, S; Mench, M; Witters, N; Vangronsveld, J

    2013-11-15

    Gentle Remediation Options (GRO) are risk management strategies or techniques for contaminated sites that result in no gross reduction in soil functionality (or a net gain) as well as risk management. Intelligently applied GROs can provide: (a) rapid risk management via pathway control, through containment and stabilisation, coupled with a longer term removal or immobilisation/isolation of the contaminant source term; and (b) a range of additional economic (e.g. biomass generation), social (e.g. leisure and recreation) and environmental (e.g. CO2 sequestration) benefits. In order for these benefits to be optimised or indeed realised, effective stakeholder engagement is required. This paper reviews current sector practice in stakeholder engagement and its importance when implementing GRO and other remediation options. From this, knowledge gaps are identified, and strategies to promote more effective stakeholder engagement during GRO application are outlined. Further work is required on integrating stakeholder engagement strategies into decision support systems and tools for GRO (to raise the profile of the benefits of effective stakeholder engagement and participation, particularly with sector professionals), and developing criteria for the identification of different stakeholder profiles/categories. Demonstrator sites can make a significant contribution to stakeholder engagement via providing evidence on the effectiveness of GRO under varying site contexts and conditions. Effective and sustained engagement strategies however will be required to ensure that site risk is effectively managed over the longer-term, and that full potential benefits of GRO (e.g. CO2 sequestration, economic returns from biomass generation and "leverage" of marginal land, amenity and educational value, ecosystem services) are realised and communicated to stakeholders.

  19. Wielcy tego świata w twórczości poetyckiej Wisławy Szymborskiej

    OpenAIRE

    Dworakowska, Dominika

    2005-01-01

    Dieser Artikel zeigt die „großen Leute” in dichterischem Schaffen von Wisława Szymborska, ln den Kreis der Betrachtung zieht die Autorin vom Referat die Integration des polonistischen Wissen mit der Musik, der Malerei und der Philosophie. Die Verfasserin vom Artikel betonte auch, daß diese Integration in gleichzeitiger Oberschule die große Role spielt. Zadanie pt. „Digitalizacja i udostępnienie w Cyfrowym Repozytorium Uniwersytetu Łódzkiego kolekcji czasopism naukowych wydawanych ...

  20. OMX - a novel high speed and high resolution microscope and its application to nuclear and chromosomal structure analysis

    OpenAIRE

    Haase, Sebastian

    2008-01-01

    Im Rahmen dieser Arbeit wurde ein neuartiges 3D Fluoreszenz Mikroskop, OMX gennant, entworfen und gebaut. Ein umfassender Design-Neuansatz erlaubt es den neuen Anforderungen der aktuellen Biologie bezüglich erhöhter Auflösung in Zeit und Raum Rechnung zu tragen. Mit Ausnahme vom Auflegen des Objektträgers sind alle Aspekte des Mikroskops Computer-gesteuert. Einen Großteil der Software floß in ein neues, eigenständiges Open-Source Projekt ein. Es erlaubt die Verarbeitung sehr großer, ...

  1. Mollusken im Auengrünland des Biosphärenreservates Mittelelbe vor und nach dem extremen Sommerhochwasser 2002

    OpenAIRE

    2013-01-01

    Hochwasserereignisse sind von besonderer Bedeutung, da sie die Auenlandschaft räumlich und zeitlich strukturieren und so eine große Vielfalt an Habitaten schaffen. Mollusken sind von großem Artenreichtum, der in den mitteleuropäischen Flussauen am höchsten ist. Sie sind relativ leicht zu determinieren und besitzen zudem eine geringe Mobilität und dementsprechend kleine Minimalareale. Darüber hinaus sind Ökologie und Habitatansprüche der meisten Arten gut bekannt. Dadurch eignen sich Mollusken...

  2. Improving cellular robustness and butanol titers of Clostridium acetobutylicum ATCC824 by introducing heat shock proteins from an extremophilic bacterium.

    Science.gov (United States)

    Liao, Zhengping; Zhang, Yanan; Luo, Sheng; Suo, Yukai; Zhang, Shaozhi; Wang, Jufang

    2017-06-20

    In recent years, increasing concerns over environment, energy and climate have renewed interest in biotechnological production of butanol. However, growth inhibition by fermentation products and inhibitory components from raw biomass has hindered the development of acetone-butanol-ethanol (ABE) fermentation. Improving the cellular robustness of Clostridium acetobutylicum is of great importance for efficient ABE production. In this study, we attempted to improve the robustness and butanol titers of C. acetobutylicum ATCC824 by overexpressing GroESL and DnaK from the extremely radioresistant bacterium Deinococcus wulumuqiensis R12 and from C. acetobutylicum ATCC824 itself. Three recombinant strains were obtained and designated 824(dnaK R12), 824(groESL R12) and 824(groESL824). These three recombinants were found to have significantly improved tolerances to stresses including butanol, furfural, oxidation and acid. Meanwhile, the butanol titers increased to 13.0g/L, 11.2g/L and 10.7g/L, which were 49.4%, 28.7% and 23.0% higher than that from the wild-type strain (8.7g/L), respectively. For 824(dnaK R12), the production of acetic and butyric acids decreased by 97.1% (1.4g/L vs. 0.04g/L) and 100% (0.3g/L vs. 0g/L), respectively, compared with the wild-type strain. Overexpressing GroESL and DnaK from D. wulumuqiensis R12 also resulted in better growth and ABE production than the wild-type strain on fermentation in the presence of 2.5g/L furfural. Strain 824(groESL R12) was superior to 824(groESL 824) in diverse types of stress-tolerance and butanol titer, indicating that GroESL from the extremophilic bacterium could perform its function more efficiently in the heterologous host than native GroESL. Our study provides evidence that extremophilic bacteria can be excellent resources for engineering C. acetobutylicum to improve its robustness and butanol titer. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Herausforderungen durch die deutsche Wiedervereinigung

    Science.gov (United States)

    Stäglin, Reiner

    Die Wiedervereinigung stellte auch die Statistik vor große Aufgaben. Die als Organ der staatlichen Planung staatsnah orientierte Statistik der DDR musste auf das zur Neutralität und wissenschaftlichen Unabhängigkeit verpflichtete System der Bundesrepublik umgestellt werden. Ebenso verlangten die Universitäten eine Neuorientierung. Die Deutsche Statistische Gesellschaft hat sich vor allem dreier Aufgaben mit großem Engagement, aber auch mit Bedachtsamkeit angenommen: Aufnahme und Integration der Statistiker aus den neuen Bundesländern in die Gesellschaft, Begleitung der Neuausrichtung des Faches Statistik an deren Hochschulen und Sicherung sowie Nutzung von Datenbeständen der ehemaligen DDR.

  4. Die „Neue Ökonomie“ des industriellen Kapitalismus

    DEFF Research Database (Denmark)

    Reckendrees, Alfred

    2015-01-01

    Als die Industrialisierung um 1840 in den meisten deutschen Regionen begann, arbeiteten in Aachen bereits mehr als die Hälfte aller Beschäftigten in großen Fabriken. Dampfmaschinen und rauchende Schornsteine prägten das Bild der Stadt. Wie kam es dazu? Welche Veränderungen brachte die Industriewi......Als die Industrialisierung um 1840 in den meisten deutschen Regionen begann, arbeiteten in Aachen bereits mehr als die Hälfte aller Beschäftigten in großen Fabriken. Dampfmaschinen und rauchende Schornsteine prägten das Bild der Stadt. Wie kam es dazu? Welche Veränderungen brachte die...

  5. Einleitung

    Science.gov (United States)

    Walther-Klaus, Ellen

    Deutschland hat sich in den letzten Jahrzehnten den internationalen Ruf erworben, auf dem Gebiet der Technologie führend zu sein. Große, weitreichende Erfindungen sind in Deutschland gemacht worden. Viele Nobelpreise gehen nach Deutschland: Robert Bosch, Nikolaus Otto und Konrad Zuse gehören genauso zu den großen Erfindern, wie Melitta Bentz und Emmy Noether. 85 % aller Nobelpreise, die seit 1901 nach Deutschland gingen, sind in den Kategorien Chemie, Physik und Medizin vergeben worden. Allein im Jahr 2007 wurden in Deutschland fast 50.000 Patente angemeldet.

  6. Gamma ray observatory productivity showcase

    Science.gov (United States)

    Davis, R. L.; Molgaard, D. A.

    1985-01-01

    The Gamma Ray Observatory (GRO) Program has been proclaimed to be the showcase productivity program for NASA and TRW. Among the multiple disciplines of a large-scale program, there is opportunity and need for improved efficiency, effectiveness, and reduction in the cost of doing business. The efforts and tools that will or have been implemented to achieve this end are described. Since the GRO Program is mainly an engineering program with the build of one satellite, the primary emphasis is placed on improving the efficiency and quality of management and engineering.

  7. Rezension zu: İpek Çalışlar: Mrs. Atatürk. Berlin: Orlanda Verlag 2008

    Directory of Open Access Journals (Sweden)

    Susanne Benöhr-Laqueur

    2009-07-01

    Full Text Available Die 2006 erschienene Biographie über Latife Uşşaki, die geschiedene Ehefrau Mustafa Kemal Atatürks, erregte in der Türkei große Aufmerksamkeit. Das hochgelobte Buch rekonstruierte anhand zahlreicher Fakten die Ehe zweier starker Persönlichkeiten, die nach kurzer Zeit zerbrach. Die stark gekürzte deutsche Übersetzung ist sowohl aufgrund diverser formeller als auch inhaltlicher Defizite nur mit großen Einschränkungen zu empfehlen.

  8. Detection of Bartonella spp. DNA in clinical specimens using an internally controlled real-time PCR assay

    NARCIS (Netherlands)

    Bergmans, Anneke M C; Rossen, John W A

    2013-01-01

    Bartonella henselae is the causative agent of cat-scratch disease (CSD), usually presenting itself as a -self-limiting lymphadenopathy. In this chapter an internally controlled Taqman probe-based real-time PCR targeting the groEL gene of Bartonella spp. is described. This assay allows for the rapid,

  9. Examining growth, yield and bean quality of Ethiopian coffee trees

    NARCIS (Netherlands)

    Bote, Adugna

    2016-01-01

    Coffee (Coffeaarabica L.)bean production and quality are determined by a diversity of interacting factors (e.g. shade, nitrogen, crop traits). Bean yield increases with increase in radiation, but adequate fertilizer suppliesare needed to sustain the productivity. This thesis analysed coffee tree gro

  10. Heritability of cortisol response to confinement stress in European sea bass dicentrarchus labrax

    NARCIS (Netherlands)

    Volckaert, F.A.M.; Hellemans, B.; Batargias, C.; Louro, B.; Massault, C.; Houdt, Van J.K.J.; Haley, C.; Koning, de D.J.; Canario, A.V.M.

    2012-01-01

    Background: In fish, the most studied production traits in terms of heritability are body weight or growth, stress or disease resistance, while heritability of cortisol levels, widely used as a measure of response to stress, is less studied. In this study, we have estimated heritabilities of two gro

  11. NCBI nr-aa BLAST: CBRC-DNOV-01-2844 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-2844 gb|AAI20749.1| Growth hormone releasing hormone receptor [Mus musculus] gb|AAI20775.1| Gro...wth hormone releasing hormone receptor [Mus musculus] AAI20749.1 1e-114 53% ...

  12. Glucoamylase production in batch, chemostat and fed-batch cultivations by an industrial strain of Aspergillus niger

    DEFF Research Database (Denmark)

    Pedersen, Henrik; Beyer, Michael; Nielsen, Jens

    2000-01-01

    The Aspergillus niger strain BO-1 was grown in batch, continuous (chemostat) and fed-batch cultivations in order to study the production of the extracellular enzyme glucoamylase under different growth conditions. In the pH range 2.5-6.0, the specific glucoamylase productivity and the specific gro...

  13. Active Vision for Humanoid Robots

    NARCIS (Netherlands)

    Wang, X.

    2015-01-01

    Human perception is an active process. By altering its viewpoint rather than passively observing surroundings and by operating on sequences of images rather than on a single frame, the human visual system has the ability to explore the most relevant information based on knowledge, therefore when gro

  14. Monitoring the activity variations of galactic X-ray sources with WATCH on EURECA

    DEFF Research Database (Denmark)

    Brandt, Søren; Lund, N.

    1995-01-01

    sources the observation periods extended over more than 100 days. A number of X-ray transients with durations between one and five days were discovered, and, additionally two long duration X-ray transients (GRS 1915+10 and GRO J0422+32) were active and could be monitored. Towards the end of the mission...

  15. NCBI nr-aa BLAST: CBRC-MMUS-06-0057 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-06-0057 gb|AAI20749.1| Growth hormone releasing hormone receptor [Mus musculus] gb|AAI20775.1| Gro...wth hormone releasing hormone receptor [Mus musculus] AAI20749.1 0.0 100% ...

  16. NCBI nr-aa BLAST: CBRC-RNOR-04-0139 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-04-0139 gb|AAI20749.1| Growth hormone releasing hormone receptor [Mus musculus] gb|AAI20775.1| Gro...wth hormone releasing hormone receptor [Mus musculus] AAI20749.1 0.0 85% ...

  17. Environ: E00843 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00843 Coffee Coffea Beverage of Rubiaceae (madder family) Coffea seed roasted, gro...und, and extracted with hot water or cold water CYP1A2 inhibitor [HSA:1544] (Coffee contains Caffeine [DR:D00528] that inhibits CYP1A2.) ...

  18. Molecular cloning of porcine chemokine CXC motif ligand 2 (CXCL2) and mapping to the SSC8

    Science.gov (United States)

    Maternal recognition of pregnancy is accompanied by inflammatory responses with leukocytosis and increased levels of cytokines and chemokines. Human trophoblast cells secrete chemokine CXC motif ligand 1 (CXCL1)/Gro-a and other chemotactic proteins, while monocytes co-cultured with trophoblast cells...

  19. Kuidas õppida säilitama vanu tõuge? / Silvia Lotman

    Index Scriptorium Estoniae

    Lotman, Silvia, 1980-

    2006-01-01

    Norra teadlase Gro Bjırnstadti doktoritööst "Genetic Diversity of Norwegian Horses with Emphasis on Native Breeds" (Norra hobusetõugude geneetiline mitmekesisus rõhuasetusega kohalikel tõugudel). Ettekanne konverentsil "Milleks ja kuidas säilitada põliseid hobusetõuge?" Penijõel 15. novembril 2003. aastal

  20. The effects of infant orthopaedics on speech and language development in children with unilateral cleft lip and palate

    NARCIS (Netherlands)

    Konst, Emmy Maria

    2002-01-01

    This thesis describes an investigation into the effects of infant orthopaedics (IO) on speech and language development in children with unilateral cleft lip and palate (UCLP). The study was performed within the framework of the three-centre prospective randomised clinical trial 'Dutchcleft'. Two gro

  1. Response of strawberry cvs. Ventana and Camarosa to salinity and specific ion composition of irrigation water

    Science.gov (United States)

    Strawberry is considered to be one of the most salt sensitive of all crops. Strawberries grown in southern and central California are located primarily in the coastal valleys. There is recent interest in developing production in inland valleys, due to their potential for early market production. Gro...

  2. Long-Term Cost-Effectiveness and Return-on-Investment of a Mindfulness-Based Worksite Intervention

    NARCIS (Netherlands)

    Dongen, J.M. van; Berkel, J. van; Boot, C.R.L.; Bosmans, J.E.; Proper, K.I.; Bongers, P.M.; Beek, A.J. van der; Tulder, M.W. van; Wier, M.F. van

    2016-01-01

    Objectives: The aim of this study was to conduct a cost-effectiveness and return-on-investment analysis comparing a mindfulness-based worksite intervention to usual practice. Methods: Two hundred fifty-seven governmental research institute employees were randomized to the intervention or control gro

  3. the effect of milk supplementation on the development and ...

    African Journals Online (AJOL)

    BSN

    produce lactic acid varies depending on the strain and conditions for growth. ... The milk industries are often faced with the problem of variation of milk compost io11. ... milk wilh yeast extracts on lhe gro"'th and acidification of lactic bacteria.

  4. Sie lebt! Zur Verbraucherforschung im deutschsprachigen Raum

    DEFF Research Database (Denmark)

    Oehler, Andreas; Reisch, Lucia

    2012-01-01

    in der Zukunft) führt mit großem Abstand der Themenbereich „Nachhaltigkeit/Ressourcenschonung/nachhaltiger, ökologisch-sozialer Konsum“ an (62 %). Insbesondere bei „Finanzdienstleistungen“, den „Märkten der sozialen Sicherung“ und dem „Datenschutz“ geben deutlich mehr Forscher eine Dringlichkeit an als...

  5. Der Shakeout im Industrielebenszyklus

    DEFF Research Database (Denmark)

    Croonenbroeck, Carsten; Grimpe, Christoph; Stadtmann, Georg

    In vormals aufstrebenden Branchen wie z. B. der Solarindustrie kommt es plötzlich zu einem massiven Austritt selbst großer Marktteilnehmer. Warum? Dieser Artikel zeigt, wie mit mikroökonomischen Überlegungen argumentiert werden kann, um die Anzahl an Unternehmen in einem Markt zu bestimmen. Es wi...

  6. Wortbildungsangaben als Hilfe für den Zugriff auf andere Datentypen und als Hilfe bei kommunikativen und kognitiven Informationsbedürfnissen

    DEFF Research Database (Denmark)

    Bergenholtz, Henning

    2013-01-01

    "Grundsätzlich können unendlich umfangreiche Mengen" von Daten eingespeist werden, schreiben Ulrike Haß und Ulrich Schmitz in der Einleitung zum Themaheft "Lexikographie im Internet" (Lexicographica 26, 2010). Das ist zwar nicht korrekt, da man zwar enorm große Mengen in einer lexikographischen D...

  7. Das Tibetische Totenbuch : Vom tibetischen Ritualtext zum spirituellen Klassiker

    NARCIS (Netherlands)

    Rakow, K.

    2008-01-01

    Der Artikel befasst sich mit der Transformation tibetisch-buddhistischer Ritualtexte in einen universellen, spirituellen Klassiker, der unter dem Titel Das Tibetische Totenbuch große Bekanntheit erlangte und den westlichen Menschen die ‚Kunst des Lebens und des Sterbens’ lehren soll. Den Ausgangspun

  8. Modelling of LEG tilting pad journal bearings with active lubrication

    DEFF Research Database (Denmark)

    Cerda Varela, Alejandro Javier; García, Asier Bengoechea; Santos, Ilmar

    2017-01-01

    This work constitutes the first step in a research effort aimed at studying the feasibility of introducing an active lubrication concept in tilting pad journal bearings (TPJBs) that feature the leading edge groove (LEG) lubrication system. The modification of the oil flow into each pad supply gro...

  9. InterProScan Result: CN374573 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available CN374573 CN374573_1_ORF2 E80B182A29F5AA6F PANTHER PTHR23256:SF268 TYROSINE PROTEIN KINASE FES (FPS) (PTK GRO...UP IV) 9.9e-17 T IPR020764 Tyrosine-protein kinase, non-receptor Fes Molecular Func

  10. InterProScan Result: FS761468 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS761468 FS761468_2_ORF1 3407DC4A7F2A2F17 PANTHER PTHR23256:SF268 TYROSINE PROTEIN KINASE FES (FPS) (PTK GRO...UP IV) 7.9e-33 T IPR020764 Tyrosine-protein kinase, non-receptor Fes Molecular Func

  11. InterProScan Result: FS761468 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS761468 FS761468_2_ORF1 3407DC4A7F2A2F17 PANTHER PTHR23256:SF268 TYROSINE PROTEIN KINASE FES (FPS) (PTK GRO...UP IV) NA ? IPR020764 unintegrated Molecular Function: non-membrane spanning protei

  12. Kuidas õppida säilitama vanu tõuge? / Silvia Lotman

    Index Scriptorium Estoniae

    Lotman, Silvia, 1980-

    2006-01-01

    Norra teadlase Gro Bjırnstadti doktoritööst "Genetic Diversity of Norwegian Horses with Emphasis on Native Breeds" (Norra hobusetõugude geneetiline mitmekesisus rõhuasetusega kohalikel tõugudel). Ettekanne konverentsil "Milleks ja kuidas säilitada põliseid hobusetõuge?" Penijõel 15. novembril 2003. aastal

  13. Gait and upper limb variability in Parkinson's disease patients with and without freezing of gait

    NARCIS (Netherlands)

    Barbe, M.T.; Amarell, M.; Snijders, A.H.; Florin, E.; Quatuor, E.L.; Schonau, E.; Fink, G.R.; Bloem, B.R.; Timmermann, L.

    2014-01-01

    Patients with Parkinson's disease (PD) and freezing of gait (FOG) (freezers) demonstrate high gait variability. The objective of this study was to determine whether freezers display a higher variability of upper limb movements and elucidate if these changes correlate with gait. We were the first gro

  14. The influence of motor cortical stimulus intensity on the relaxation rate of human lower limb muscles

    NARCIS (Netherlands)

    McNeil, C.J.; Bredius, M.S.; Molenaar, J.P.F.; Gandevia, S.C.

    2013-01-01

    Transcranial magnetic stimulation (TMS) allows an in vivo assessment of the rate of muscle relaxation during a voluntary contraction. It is unknown if this method can be applied to lower limb muscles, and the effect of stimulus intensity on relaxation rate has not been investigated in any muscle gro

  15. Pursuing sustainable development in Norway: the challenge of living up to Brundtland at home

    NARCIS (Netherlands)

    Lafferty, William M.; Knudsen, Jørgen; Larsen, Olav Mosvold

    2007-01-01

    With the Norwegian Prime Minister, Gro Harlem Brundtland, as chair of the World Commission on Environment and Development, Norway became an early mover in politics for sustainable development (SD). The pursuit of SD goals has been expressed in several national policy documents, though it was not unt

  16. An evaluation of multiplex bead-based analysis of cytokines and soluble proteins in archived lithium heparin plasma, EDTA plasma and serum samples

    DEFF Research Database (Denmark)

    Brøndum, Line; Sørensen, Brita Singers; Eriksen, Jesper Grau

    2016-01-01

    in plasma and serum from 86 head and neck cancer patients and 33 controls were evaluated: EGFR, leptin, OPN, VEGFR-1, VEGFR-2, IL-2, IL-13, PDGF-bb, TNF, PAI-1, SDF-1a, IL-4, IL-6, IL-8, eotaxin, G-CSF, VEGF, GRO-a, and HGF. RESULTS: The correlation between measurements of the same samples analyzed...

  17. SwissProt search result: AK120503 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK120503 J013122K17 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-141 ...

  18. SwissProt search result: AK063576 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK063576 001-117-H02 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 3e-85 ...

  19. SwissProt search result: AK069617 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK069617 J023019K12 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 0.0 ...

  20. SwissProt search result: AK060117 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK060117 006-308-E09 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 2e-71 ...

  1. SwissProt search result: AK073999 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK073999 J033073C08 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-177 ...

  2. SwissProt search result: AK070127 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK070127 J023045O03 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-157 ...

  3. SwissProt search result: AK062098 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK062098 001-045-A02 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-137 ...

  4. SwissProt search result: AK070603 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK070603 J023059D06 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-150 ...

  5. SwissProt search result: AK119217 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK119217 001-046-E10 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-113 ...

  6. SwissProt search result: AK100602 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK100602 J023107D12 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-108 ...

  7. SwissProt search result: AK101537 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK101537 J033048E22 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-151 ...

  8. SwissProt search result: AK060773 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK060773 001-033-B08 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 2e-53 ...

  9. SwissProt search result: AK101334 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK101334 J033034I03 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-142 ...

  10. SwissProt search result: AK061901 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK061901 001-041-H05 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 7e-75 ...

  11. SwissProt search result: AK119223 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK119223 001-100-D06 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 5e-71 ...

  12. SwissProt search result: AK109517 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK109517 002-109-A02 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 0.0 ...

  13. SwissProt search result: AK061410 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK061410 006-306-B08 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 2e-63 ...

  14. SwissProt search result: AK120234 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK120234 J013043L17 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-13 ...

  15. SwissProt search result: AK068562 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK068562 J013152E19 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-127 ...

  16. SwissProt search result: AK119623 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK119623 002-117-H08 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 0.0 ...

  17. SwissProt search result: AK068277 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK068277 J013146G10 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 2e-77 ...

  18. SwissProt search result: AK108892 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK108892 002-152-E06 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 1e-139 ...

  19. SwissProt search result: AK101042 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK101042 J033003J10 (P35635) 60 kDa chaperonin (Protein Cpn60) (groEL protein) (Immunoreactive... protein Bb65) (Immunoreactive protein Bb63) (Heat shock protein 60) (HSP 60) CH60_BARBA 2e-20 ...

  20. Statins affect the presentation of endothelial chemokines by targeting to multivesicular bodies.

    Directory of Open Access Journals (Sweden)

    Johanna Hol

    Full Text Available BACKGROUND: In addition to lowering cholesterol, statins are thought to beneficially modulate inflammation. Several chemokines including CXCL1/growth-related oncogene (GRO-α, CXCL8/interleukin (IL-8 and CCL2/monocyte chemoattractant protein (MCP-1 are important in the pathogenesis of atherosclerosis and can be influenced by statin-treatment. Recently, we observed that atorvastatin-treatment alters the intracellular content and subcellular distribution of GRO-α in cultured human umbilical vein endothelial cells (HUVECs. The objective of this study was to investigate the mechanisms involved in this phenomenon. METHODOLOGY/ PRINCIPAL FINDINGS: The effect of atorvastatin on secretion levels and subcellular distribution of GRO-α, IL-8 and MCP-1 in HUVECs activated by interleukin (IL-1β were evaluated by ELISA, confocal microscopy and immunoelectron microscopy. Atorvastatin increased the intracellular contents of GRO-α, IL-8, and MCP-1 and induced colocalization with E-selectin in multivesicular bodies. This effect was prevented by adding the isoprenylation substrate GGPP, but not the cholesterol precursor squalene, indicating that atorvastatin exerts these effects by inhibiting isoprenylation rather than depleting the cells of cholesterol. CONCLUSIONS/ SIGNIFICANCE: Atorvastatin targets inflammatory chemokines to the endocytic pathway and multivesicular bodies and may contribute to explain the anti-inflammatory effect of statins at the level of endothelial cell function.

  1. The Indirect Approach to Counterterrorism

    Science.gov (United States)

    2008-01-01

    and societal deterioration. l1 Today, Somalia continues to degenerate into a breeding groUnd and safe haven for ten-orist organizations while local...people and Zimbabwe has become a transit point for cannabis , heroin, and 9 methamphetamines destined for South Africa?O Additionally, the expansion of

  2. Inclusion of Pedigree-analysis (NUSAP) in undergraduate science education: An example

    DEFF Research Database (Denmark)

    Dam, Marie Inger; Kjær Jensen, Jon; Jónsson, Jóhann Gunnar

    2009-01-01

    Aalborg University applies a problem-based learning model. Each semester all students form groups. Group members formulate and agree upon a problem they want to address, within their course of study, and write a long report on their findings (length depends on the year of study, the number of gro...

  3. Economic values of growth and feed efficiency for fish farming in recirculating aquaculture system with density and nitrogen output limitations: a case study with African catfish (Clarias gariepinus)

    NARCIS (Netherlands)

    Besson, M.; Komen, H.; Aubin, J.; Boer, de I.J.M.; Poelman, M.; Quillet, E.; Vancoillie, C.; Vandeputte, M.; Arendonk, van J.A.M.

    2014-01-01

    In fish farming, economic values (EV) of breeding goal traits are lacking, even though they are key parameters when defining selection objectives. The aim of this study was to develop a bioeconomic model to estimate EV of 2 traits representing production performances in fish farming: the thermal gro

  4. Polymerisation of Beta-alanine through catalytic ester-amide exchange

    NARCIS (Netherlands)

    Steunenberg, P.; Könst, P.M.; Scott, E.L.; Franssen, M.C.R.; Zuilhof, H.; Sanders, J.P.M.

    2013-01-01

    Herein we present the use of group (IV) metal alkoxides as catalysts for the polymerisation of esters of p-alanine and its derivatives. The influence of different group (IV) metal alkoxides, different esters, temperature and solvents on the polymerisation are investigated. The order in which the gro

  5. The rotator cuff: from bench to bedside. Developments in tissue engineering, surgical techniques and pathogenetic factors

    NARCIS (Netherlands)

    Longo, U.G.

    2012-01-01

    This thesis originates from the difficulties in the management of patients with rotator cuff tears. Since tendon healing rate is relatively slow compared with other connective tissues, we reviewed the available literature on tissue engineered biological augmentation for tendon healing, including gro

  6. An ultra-dense SNP linkage map for the octoploid, cultivated strawberry and its application in genetic research

    Science.gov (United States)

    We will present an ultra-dense genetic linkage map for the octoploid, cultivated strawberry (Fragaria x ananassa) consisting of over 13K Axiom® based SNP markers and 150 previously mapped reference SSR loci. The high quality of the map is demonstrated by the short sizes of each of the 28 linkage gro...

  7. (YIP 09) Domain Engineered Magnetoelectric Thin Films for High Sensitivity Resonant Magnetic Field Sensors

    Science.gov (United States)

    2011-12-01

    hybrid te can be ac ia optimize hybrid depo ature, micr . BaTiO3(BTO nd microstru r deposition for as-gro dopted the s with (111 re also fou he...coupling. F ure and its f deposition ( addition, hig ited PZT fi 100) highly ized silicon tion of micr restingly, th pyramid to was incre the

  8. Molecular analyses of a potentially novel Anaplasma species closely related to Anaplasma phagocytophilum detected in sika deer (Cervus nippon yesoensis) in Japan.

    Science.gov (United States)

    Ybañez, Adrian P; Matsumoto, Kotaro; Kishimoto, Toshio; Inokuma, Hisashi

    2012-05-25

    An Anaplasma species closely related to Anaplasma phagocytophilum detected in sika deer in Hokkaido, Japan was molecularly analyzed using 16S rRNA, citrate synthase (gltA), and heat-shock operon (groEL) gene sequences. Genome walking was performed to determine its complete gltA and groEL sequences (1233 bp and 1650 bp, respectively). Percent identities to the closest A. phagocytophilum sequences from the US and European strains were 98.6-98.8%, 76.5%, and 80.3-80.8% for 16S rRNA, gltA, and groEL genes, respectively. For deduced amino acid sequences, percent identities to the closest A. phagocytophilum sequences were 66.7% and 97.6% for gltA and groEL genes, respectively. Phylogenetic analyses revealed divergence from any known A. phagocytophilum strain. The lower identities and the divergent phylogenetic position of the Anaplasma sp. detected from sika deer in Japan with established A. phagocytophilum strains provide evidence of its potential novelty. Copyright © 2011 Elsevier B.V. All rights reserved.

  9. Referral Directory System Specification

    Science.gov (United States)

    1990-03-30

    Center, Ship Materiall I IDavid W. Taylor Naval Ship Research and Development Center, Technical Infi *Defense Audiovisual Agency, Still Photographic...and gro und life support, aerospace medicine, human performance , medical equipment, dental service operations, bioeffe cts-radiation and chemical...Combined Arms Support Laboratory 990756 Army Infantry School Library 990920 Army Communications Systems Center 990785 Army Institute of Dental Research

  10. Injuries in Children with Extra Physical Education in Primary Schools

    DEFF Research Database (Denmark)

    Rexen, Christina; Andersen, Lars Bo; Ersbøll, Annette Kjær

    2014-01-01

    (1) Examine the influence of extra physical education (EPE) on the number of musculoskeletal injuries in public schools accounting for organized sports participation (OSP) outside school. (2) Examine the major injury subgroup: growth-related overuse (GRO) through the overuse-related injury group....

  11. A Multi-Walled Carbon Nanotube-based Biosensor for Monitoring Microcystin-LR in Sources of Drinking Water Supplies

    Science.gov (United States)

    A multi-walled carbon nanotube-based electrochemical biosensor is developed for monitoring microcystin-LR (MC-LR), a toxic cyanobacterial toxin, in sources of drinking water supplies. The biosensor electrodes are fabricated using dense, mm-long multi-walled CNT (MWCNT) arrays gro...

  12. Trust : A Partnership's Must

    NARCIS (Netherlands)

    Kolsteeg, Johan; Hagoort, Giep

    2016-01-01

    How to realize effective cooperations and spillovers (Schramme, 2014) between creative and non-creative firms has been a subject of political debate in the Netherlands for years. Several subsequent governmental programs have attempted to ignite the sequence of creativity, innovation and economic gro

  13. Cross-cultural perception and power dynamics across changing organizational and national contexts : Curaçao and the Netherlands

    NARCIS (Netherlands)

    Heijes, Cornelis

    2011-01-01

    In this article we study the role of power and power differences in cross-cultural perception. We do so by way of exploratory case studies in the Internal Revenue Service (IRS) and the police in the Netherlands and Curacao. We demonstrate how cross-cultural perception between two specific ethnic gro

  14. of the kudu, Tragelaphus strepsiceros, in an agricultural

    African Journals Online (AJOL)

    Rome, Food and Agricultural Organization of the United Nations, ... observed on the Springbok Flats study area between March and October. 1985. III. N iii. 11-. :;) 0. II: CI. 7.0. 6.0 ... Tooth eruption, groW1h and ageing criteria in greater kudu ...

  15. 78 FR 29388 - Notice of Intent To Grant Exclusive License

    Science.gov (United States)

    2013-05-20

    ... Case No. MSC-25633-1, to GRoK Technologies, LLC, having its principal place of business in Houston... represented by the Administrator of the National Aeronautics and Space Administration. The prospective... prospective exclusive license may be granted unless within fifteen (15) days from the date of this published...

  16. Generalized Fourier transforms Fk,a

    DEFF Research Database (Denmark)

    Salem, Ben Said; Kobayashi, Toshiyuki; Ørsted, Bent

    2009-01-01

    We construct a two-parameter family of actions ωk,a of the Lie algebra by differential-difference operators on . Here, k is a multiplicity-function for the Dunkl operators, and a>0 arises from the interpolation of the Weil representation and the minimal unitary representation of the conformal gro...

  17. Of yeast and mushrooms : Patterns of industry-level productivity growth

    NARCIS (Netherlands)

    Inklaar, Robert; Timmer, Marcel P.

    2007-01-01

    In this paper we analyse labour productivity growth in the United States, four European countries (France, Germany, the Netherlands and United Kingdom), Australia and Canada between 1987 and 2003 from an industry perspective. Rather than analysing broad industry groups, we compare the pattern of gro

  18. Does a smoking prevention program in elementary schools prepare children for secondary school?

    NARCIS (Netherlands)

    Crone, M.R.; Spruijt, R.; Dijkstra, N.S.; Willemsen, M.C.; Paulussen, T.G.W.M.

    2011-01-01

    A smoking prevention program was developed to prepare children in elementary school for secondary school. This study assessed the effects on smoking in secondary school. Methods: In 2002, 121 schools in The Netherlands were randomly assigned to the intervention or control group. The intervention gro

  19. A controlled clinical trial of implantretained mandibular overdentures : 10 years’ results of clinical aspects and aftercare of IMZ implants and Bra°nemark implants

    NARCIS (Netherlands)

    Meijer, Henny J.A.; Raghoebar, Gerry M.; Van’t Hof, Martin A.; Visser, Anita

    2004-01-01

    The aim of this prospective randomized controlled clinical trial was to evaluate the clinical outcomes and prosthetic aftercare of edentulous patients with a mandibular overdenture retained by two IMZ implants or two Bra°nemark implants during a 10-year period. Patients were allocated to the IMZ gro

  20. A caudal proliferating growth center contributes to both poles of the forming heart tube

    NARCIS (Netherlands)

    van den Berg, G.; Abu-Issa, R.; de Boer, B.A.; Hutson, M.R.; de Boer, P.A.J.; Soufan, A.T.; Ruijter, J.M.; Kirby, M.L.; van den Hoff, M.J.B.; Moorman, A.F.M.

    2009-01-01

    Recent studies have shown that the primary heart tube continues to grow by addition of cells from the coelomic wall. This growth occurs concomitantly with embryonic folding and formation of the coelomic cavity, making early heart formation morphologically complex. A scarcity of data on localized gro

  1. Home care in Europe: growing interest among decision makers, but little information available for policy development.

    NARCIS (Netherlands)

    Genet, N.

    2010-01-01

    Research problem: Integrated systems of home care are assumed to be an adequate response to current and future challenges to health and social services that result from demographic and social developments. The interest in home care systems and the willingness to learn from foreign experiences is gro

  2. Ongelijkheid in Nederland

    NARCIS (Netherlands)

    Salverda, W.

    2014-01-01

    Een goede vergelijking van de ongelijkheid in Nederland met de uitkomsten van Piketty’s onderzoek is niet goed mogelijk, al was het maar omdat onze cijfers over vermogens niet verder teruggaan dan de jaren negentig. Maar duidelijk is dat de Nederlandse vermogensongelijkheid internationaal gezien gro

  3. Randomized Trial on the Effectiveness of Dexamethasone in TMJ Arthrocentesis

    NARCIS (Netherlands)

    Huddleston-Slater, J.J.R.; Vos, L.M.; Stroy, L.P.P.; Stegenga, B.

    2012-01-01

    The aim of this study was to compare the effectiveness of dexamethasone administration following arthrocentesis of the temporomandibular joint (TMJ) with a placebo (saline). Twenty-eight participants with TMJ arthralgia were randomly assigned to two groups of a parallel double-blind RCT. In both gro

  4. DMPD: Convergence of the NF-kappaB and IRF pathways in the regulation of the innateantiviral response. [Dynamic Macrophage Pathway CSML Database

    Lifescience Database Archive (English)

    Full Text Available 17706453 Convergence of the NF-kappaB and IRF pathways in the regulation of the innateanti... (.png) (.svg) (.html) (.csml) Show Convergence of the NF-kappaB and IRF pathways in the regulation of the innateanti... IRF pathways in the regulation of the innateantiviral response. Authors Hiscott J. Publication Cytokine Gro

  5. Recent advances in alveolar biology: Evolution and function of alveolar proteins

    NARCIS (Netherlands)

    Orgeig, S.; Hiemstra, P.S.; Veldhuizen, E.J.A.; Casals, C.; Clark, H.W.; Hackzu, A.; Knudsen, L.; Possmayer, F.

    2010-01-01

    This review is focused on the evolution and function of alveolar proteins. The lung faces physical and environmental challenges, due to changing pressures/volumes and foreign pathogens, respectively. The pulmonary surfactant system is integral in protecting the lung from these challenges via two gro

  6. Helmut Thiel zum 70. Geburtstag

    OpenAIRE

    Musche, Bernd

    2013-01-01

    Am 18.03.2005 beging der Naturschutzbeauftragte des Landkreises Bernburg, Herr Helmut Thiel, seinen 70. Geburtstag, ein willkommener Anlass, den jahrzehntelangen unermüdlichen Einsatz des Jubilars für den Natur- und Umweltschutz zu würdigen und ihm vor allem für das große Engagement in der ehrenamtlichen Arbeit ganz herzlich zu danken.

  7. Restrictive dermopathy and fetal behaviour

    NARCIS (Netherlands)

    Mulder, EJH; Beemer, FA; Stoutenbeek, P

    2001-01-01

    We report three siblings from consecutive pregnancies affected with restrictive dermopathy (RD). During the second pregnancy, fetal behavioural development and growth were studied extensively using ultrasound at 1-4 week intervals. Dramatic and sudden changes occurred in fetal body movements and gro

  8. Serum cytokine/chemokine profiles in patients with dengue fever (DF) and dengue hemorrhagic fever (FHD) by using protein array.

    Science.gov (United States)

    Oliveira, Renato Antonio Dos Santos; Cordeiro, Marli Tenório; Moura, Patrícia Muniz Mendes Freire de; Baptista Filho, Paulo Neves Bapti; Braga-Neto, Ulisses de Mendonça; Marques, Ernesto Torres de Azevedo; Gil, Laura Helena Vega Gonzales

    2017-04-01

    DENV infection can induce different clinical manifestations varying from mild forms to dengue fever (DF) or the severe hemorrhagic fever (DHF). Several factors are involved in the progression from DF to DHF. No marker is available to predict this progression. Such biomarker could allow a suitable medical care at the beginning of the infection, improving patient prognosis. The aim of this study was to compare the serum expression levels of acute phase proteins in a well-established cohort of dengue fever (DF) and dengue hemorrhagic fever (DHF) patients, in order to individuate a prognostic marker of diseases severity. The serum levels of 36 cytokines, chemokines and acute phase proteins were determined in DF and DHF patients and compared to healthy volunteers using a multiplex protein array and near-infrared (NIR) fluorescence detection. Serum levels of IL-1ra, IL-23, MIF, sCD40 ligand, IP-10 and GRO-α were also determined by ELISA. At the early stages of infection, GRO-α and IP-10 expression levels were different in DF compared to DHF patients. Besides, GRO-α was positively correlated with platelet counts and IP-10 was negatively correlated with total protein levels. These findings suggest that high levels of GRO-α during acute DENV infection may be associated with a good prognosis, while high levels of IP-10 may be a warning sign of infection severity. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Assessing microsatellite linkage disequilibrium in wild, cultivated, and mapping populations of Theobroma cacao L and its impact on association mapping

    Science.gov (United States)

    Linkage disequilibrium (LD) is the nonrandom association of alleles and loci within sets of genetic data and when measured over the genomes of a species can provide important indications for how future association analyses should proceed. This information can be advantageous especially for slow-gro...

  10. Lipoprotein lipase S447X variant associated with VLDL, LDL and HDL diameter clustering in the MetS

    Science.gov (United States)

    Previous analysis clustered 1,238 individuals from the general population Genetics of Lipid Lowering Drugs Network (GOLDN) study by the size of their fasting very low-density, low-density and high-density lipoproteins (VLDL, LDL, HDL) using latent class analysis. From two of the eight identified gro...

  11. Nutrition of People with Spinal Cord Injuries

    Science.gov (United States)

    This conference proceeding summarizes current knowledge about the nutritional status and needs of the spinal cord injured patient. Topics covered include the aspects of spinal cord injury that influence nutrient intakes and status, and the nutrients most likely to be problematic in this diverse gro...

  12. Notizen zur Fortpflanzungsbiologie und Jugendentwicklung zweier Grubenottern (Serpentes: Crotalidae: Crotalus atrox und Agkistrodon p. piscivorus)

    NARCIS (Netherlands)

    Petzold, Hans Günter

    1963-01-01

    Über die postembryonale Entwicklung — besonders die Gewichtszunahme — junger Giftschlangen (Viperiden, Crotaliden, Elapiden) gibt es aus naheliegenden Gründen nur wenige eingehendere Untersuchungen. KLAUBER (1956) bringt an Hand eines großen Materials eine Zusammenfassung einschlägiger Freilandbeoba

  13. Ein Leben in Worten

    DEFF Research Database (Denmark)

    Siegumfeldt, Inge Birgitte; Auster, Paul

    Gesprächen über Austers Bücher. In einem intensiven, persönlichen Dialog erkunden sie seine großen Romane und die autobiographischen Texte. Auster gibt dabei einen intimen Einblick in seine Arbeit, erzählt amüsante Anekdoten und spricht offen wie selten über Inspirationsquellen und Motivation. Die...

  14. Setting the chaperonin timer: the effects of K+ and substrate protein on ATP hydrolysis.

    Science.gov (United States)

    Grason, John P; Gresham, Jennifer S; Widjaja, Lusiana; Wehri, Sarah C; Lorimer, George H

    2008-11-11

    The effects of potassium ion on the nested allostery of GroEL are due to increases in the affinity for nucleotide. Both positive allosteric transitions, TT-TR and TR-RR, occur at lower [ATP] as [K(+)] is increased. Negative cooperativity in the double-ringed system is also due to an increase in the affinity of the trans ring for the product ADP as [K(+)] is increased. Consequently, (i) rates of ATP hydrolysis are inversely proportional to [K(+)] and (ii) the residence time of GroES bound to the cis ring is prolonged and the hemicycle time extended. Substrate protein suppresses negative cooperativity by decreasing the affinity of the trans ring for ADP, reducing the hemicycle time to a constant minimum. The trans ring thus serves as a variable timer. ATP added to the asymmetric GroEL-GroES resting-state complex lacking trans ring ADP is hydrolyzed in the newly formed cis ring with a presteady-state burst of approximately 6 mol of Pi per mole of 14-mer. No burst is observed when the trans ring contains ADP. The amplitude and kinetics of ATP hydrolysis in the cis ring are independent of the presence or absence of encapsulated substrate protein and independent of K(+) at concentrations where there are profound effects on the linear steady-state rate. The hydrolysis of ATP by the cis ring constitutes a second, nonvariable timer of the chaperonin cycle.

  15. Parameterizing the soil - water - plant root system

    NARCIS (Netherlands)

    Feddes, R.A.; Raats, P.A.C.

    2004-01-01

    Root water uptake is described from the local scale, to the field scale and to the regional and global scales. The local macroscopic model can be incorporated in Soil-Plant-Atmosphere Continuum (SPAC) numerical models, like the SWAP, HYSWASOR, HYDRUS, ENVIRO-GRO and FUSSIM models. These SPAC models

  16. Diabetes at work. Fatigue in relation to job characteristics, diabetes symptoms and self-management

    NARCIS (Netherlands)

    Weijman, Iris

    2005-01-01

    The focus of this thesis is on employees with diabetes. Fatigue, work characteristics and diabetes self-management are core aspects. Diabetes may have many consequences in the working situation and is expected to become an even bigger health problem, because the number of people with diabetes is gro

  17. Mehrsprachigkeit - Language Awareness – Sprachbewusstheit. Einführung

    Directory of Open Access Journals (Sweden)

    Monika Angela Budde

    2016-10-01

    Ich bedanke mich bei allen AutorInnen für ihre Beiträge und für die überaus große Kooperationsbereitschaft. Diese habe ich ebenso im Herausgeberteam erfahren und auch diesem möchte ich herzlich danken.

  18. Cultural Difference and Human Rights : A Philosophical-Anthropological Approach

    NARCIS (Netherlands)

    J. Kloeg (Julien)

    2014-01-01

    textabstractIn ‘Cultural Difference and Human Rights’, Julien Kloeg claims, with Pablo Gilabert, that theoretical attempts to justify human rights should move beyond the dichotomy of providing either a humanist or a political justification. Kloeg demonstrates how philosophical anthropology could gro

  19. Stratigraphy of the type Maastrichtian – a synthesis

    NARCIS (Netherlands)

    Jagt, J.W.M.; Jagt-Yazykova, E.A.

    2012-01-01

    A synthesis of the stratigraphy of the Maastrichtian Stage in its extended type area, that is, southern Limburg (the Netherlands), and adjacent Belgian and German territories, is presented with a brief historical overview. Quarrying activities at the large quarry complex of ENCI-HeidelbergCement Gro

  20. Substrate utilization and respiration in relation to growth and maintenance in higher plants

    NARCIS (Netherlands)

    Penning de Vries, F.W.T.

    1973-01-01

    De betekenis en regulering van ademhalingsprocessen in planten zijn bestudeerd om vast te stellen hoeveel van de koolstof die door fotosynthese is vastgelegd door ademhaling weer verloren gaat. Het grootste deel van de ademhaling van veldgewassen is het gevolg van groeiprocessen. Op theoretische gro

  1. Honey bee (Apis mellifera) intracolonial genetic diversity influences worker nutritional status

    Science.gov (United States)

    Honey bee queens mate with multiple males - a reproductive strategy known as polyandry - that results in colonies comprised of high intracolonial genetic diversity among nestmates. Several studies have demonstrated the adaptive significance of polyandry for overall colony performance and colony gro...

  2. Dicty_cDB: Contig-U09246-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ) Neurospora crassa DNA linkage gro... 34 5.7 AY775061_1( AY775061 |pid:none) Copadichromis borleyi short wa...tName: Full=Blue ... 33 9.8 AY775065_1( AY775065 |pid:none) Copadichromis borleyi short wavele... 33 9.8 AY7

  3. The Radius-Luminosity Relationship for Active Galactic Nuclei: The Effect of Host-Galaxy Starlight on Luminosity Measurements. II. The Full Sample of Reverberation-Mapped AGNs

    DEFF Research Database (Denmark)

    Bentz, Misty C.; Peterson, Bradley M.; Netzer, Hagai

    2009-01-01

    We present high-resolution Hubble Space Telescope images of all 35 active galactic nuclei (AGNs) with optical reverberation-mapping results, which we have modeled to create a nucleus-free image of each AGN host galaxy. From the nucleus-free images, we determine the host-galaxy contribution to gro...

  4. Experiment list: SRX344978 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available urce_name=Input_ING1_flag_myoblasts || cell line=C2C12 || genotype/variation=ectopically expressing Flag-tagged ING1 || cell type=Gro...wing C1C12 myoblasts || chromatin preparation method=Son

  5. Abpfiff für das Fußball-Universum

    Science.gov (United States)

    Bartelmann, Matthias

    2005-03-01

    Aus Beobachtungen der kosmischen Hintergrundstrahlung lassen sich zwar Rückschlüsse auf die Größe und die Topologie des Universums ziehen. Die Behauptung, aus den Daten des Satelliten WMap würde folgen, dass das Universum endlich groß sei und die Topologie eines Dodekaeders aufweise, wurde jedoch widerlegt.

  6. Written online situational feedback via mobile phone to support self-management of chronic widespread pain: a usability study of a web-based intervention.

    NARCIS (Netherlands)

    Kristjánsdóttir, O.B.; Fors, E.A.; Eide, E.; Finset, A.; Dulmen, S. van; Wigers, S.H.; Eide, H.

    2011-01-01

    BACKGROUND: This pretrial study aimed to develop and test the usability of a four-week Internet intervention delivered by a Web-enabled mobile phone to support self-management of chronic widespread pain. METHODS: The intervention included daily online entries and individualized written feedback, gro

  7. Industrial high-rate (~14 nm/s) deposition of low resistive and transparent ZnOx:Al films on glass

    NARCIS (Netherlands)

    Illiberi, A.; Kniknie, B.; Deelen, J. van; Steijvers, H.L.A.H.; Habets, D.; Simons, P.J.P.M.; Janssen, A.C.; Beckers, E.H.A.

    2011-01-01

    Aluminum doped ZnOx (ZnOx:Al) films have been deposited on glass in an in-line industrial-type reactor by a metalorganic chemical vapor deposition process at atmospheric pressure. Tertiary-butanol has been used as oxidant for diethylzinc and trimethylaluminium as dopant gas. ZnOx:Al films can be gro

  8. Very few indications justify early treatment for severe Class II malocclusions

    NARCIS (Netherlands)

    Ren, Yijin

    2004-01-01

    DESIGN: This was a single-centre two-phased, parallel, randomised clinical trial (RCT) conducted over a period of more than 10 years. INTERVENTION: Children were enrolled who had severe (=7 mm overjet) Class II malocclusions and who were developmentally at least a year before their peak pubertal gro

  9. Scandinavian NATO Policy: The Next Five Years

    Science.gov (United States)

    1990-05-01

    leadership of Gro Harlem Brundtland , who grew in confidence and political stature as she survived and governed better than most observers in Norway expected...From 1986 to 1989, Brundtland headed a minority Labor government with the de facto support of the Center and Christian People’s parties in the center

  10. The Fundamental Issues Study within the British BMD Review

    Science.gov (United States)

    1998-02-01

    towards ’Outer Space’: a realm identified in the evocative terminology of the 1987 Brundtland report as one of the three ’global commons’, the other...New Scientist, vol.149, no.2020, 9 March 1996, p.6. 14. Gro Harlem Brundtland , Chair, Our Common Future, Oxford University Press for World Commission

  11. Empowering Women.

    Science.gov (United States)

    Brundtland, Gro Harlem

    1994-01-01

    An edited version of Prime Minister of Norway Gro Harlem Brundtland's keynote address to the International Conference on Population and Development in September 1994. This address focuses on the role played by women in population growth and emphasizes the urgency of economic and political rights and education for women. (LZ)

  12. Malaria. Can WHO roll back malaria?

    Science.gov (United States)

    Balter, M

    2000-10-20

    In October 1998, World Health Organization Director-General Gro Harlem Brundtland announced Roll Back Malaria, a multiagency crusade that aims to cut malaria mortality in half over the next 10 years. Brundtland might just be the one to pull it off, say numerous public health experts, although some researchers question whether the goal is realistic.

  13. Pursuing sustainable development in Norway: The challenge of living up to Brundtland at home

    NARCIS (Netherlands)

    Lafferty, W.M.; Knudsen, Jørgen; Larsen, Olav Mosvold

    2007-01-01

    With the Norwegian Prime Minister, Gro Harlem Brundtland, as chair of the World Commission on Environment and Development, Norway became an early mover in politics for sustainable development (SD). The pursuit of SD goals has been expressed in several national policy documents, though it was not

  14. A Multi-Walled Carbon Nanotube-based Biosensor for Monitoring Microcystin-LR in Sources of Drinking Water Supplies

    Science.gov (United States)

    A multi-walled carbon nanotube-based electrochemical biosensor is developed for monitoring microcystin-LR (MC-LR), a toxic cyanobacterial toxin, in sources of drinking water supplies. The biosensor electrodes are fabricated using dense, mm-long multi-walled CNT (MWCNT) arrays gro...

  15. Research brief : Serving Bowl Selection Biases the Amount of Food Served

    NARCIS (Netherlands)

    Kleef, van E.; Shimizu, M.; Wansink, B.

    2012-01-01

    Objective: To determine how common serving bowls containing food for multiple persons influence serving behavior and consumption and whether they do so independently of satiation and food evaluation. Methods: In this between-subjects experiment, 68 participants were randomly assigned to either a gro

  16. The evolution of Chinese policies and governance structures on environment, energy and climate

    NARCIS (Netherlands)

    S. Tsang; A. Kolk

    2010-01-01

    Although a successor to the 1997 Kyoto Protocol has not materialised yet, the 2009 Copenhagen meeting underlined the importance of China in international debates on climate and energy. This is not only based on China’s current climate emissions, but also on its expected energy use and economic gro

  17. Technological spillovers and industrial growth in Chinese regions

    NARCIS (Netherlands)

    Wang, L.; Meijers, H.H.M.; Szirmai, A.

    2013-01-01

    This paper focuses on the role of interregional technology spillovers in the process of industrial growth in Chinese regions in the period 1990-2005. Inflows of FDI increased rapidly from 1990 till 1998, slowing down thereafter. Domestic R&D investment accelerated after 1998. Regional industrial gro

  18. Model uncertainty in growth empirics

    NARCIS (Netherlands)

    Prüfer, P.

    2008-01-01

    This thesis applies so-called Bayesian model averaging (BMA) to three different economic questions substantially exposed to model uncertainty. Chapter 2 addresses a major issue of modern development economics: the analysis of the determinants of pro-poor growth (PPG), which seeks to combine high gro

  19. Gardens of Situations

    DEFF Research Database (Denmark)

    Boris, Stefan Darlan

    2011-01-01

    Neither globally, nor in Denmark are city and landscape any longer chracterized as each others opposites. Instead they appear as a diverse constellation of "fragmented urban landscapes". The city is no longer constituted by a hierarchical order with a unifies centrality from where development gro...

  20. Serological response to administration of Brucella abortus strain RB51 vaccine in beef and dairy heifers, using needle-free and standard needle-based injection systems

    Science.gov (United States)

    The purpose of this study was to compare immunologic responses of heifers vaccinated with 10**10 colony-forming units (CFU) of Brucella abortus strain RB51 (SRB51) by standard needle-and-syringe system or a needle-free injection system. Heifers were randomly assigned to control and vaccination gro...

  1. Flux growth and liquid-phase epitaxy of $Mn{6+}$-doped barium sulfate

    NARCIS (Netherlands)

    Ehrentraut, D.; Romanyuk, Y.E.; Pollnau, M.; Shim, K. B.

    2004-01-01

    We investigated the conditions for the growth of $Mn^{6+}$-doped from the ternary eutectic NaCl-KCl-CsCl solvent at temperatures of 480-600 $^{o}$C. The doping complex ion $MnO^{2-}_{4}$ can easily substitute the $SO^{2-}_{4}$ complex ion in $BaSO_{4}$ with its orthorhombic space group Pnma. The gro

  2. Ungarn seit der Wahl: Demokratie unter Druck

    NARCIS (Netherlands)

    A. Freyberg-Inan; M. Varga

    2011-01-01

    Die Verfasser zeigen, dass die Orban-Regierung legitime Probleme benennt, wie etwa Ungarns extrem niedrige Beschäftigungsrate, die mit zu den großen wirtschaftliche Schwierigkeiten des Landes beiträgt. Doch packt sie die identifizierten Probleme zu oft auf eine Weise an, die ganz nebenbei dem nation

  3. From Cultural Tourism to Creative Tourism : European Perspectives

    NARCIS (Netherlands)

    Richards, G.W.

    2002-01-01

    Cultural tourism is seen as a major growth market in Europe, although empirical evidence is still relatively sparse. An analysis is presented of the ATLAS survey data for 2002 as well as research conducted at the Rotterdam Cultural Capital Event in 2001. These data indicate that cultural tourism gro

  4. The last mile in analysing well-being and poverty: Indices of Social Development

    NARCIS (Netherlands)

    A. de Haan (Arjan); I.P. van Staveren (Irene); E. Webbink (Ellen); R. Foa (Roberto)

    2011-01-01

    textabstractDevelopment practitioners worldwide increasingly recognize the importance of informal institutions -- such as norms of cooperation, non-discrimination, or the role of community oversight in the management of investment activities -- in affecting well-being, poverty, and even economic gro

  5. Decreased bone density and treatment in patients with autosomal recessive cutis laxa.

    NARCIS (Netherlands)

    Noordam, C.; Funke, S.; Slobbe-Knoers, V.V.A.M.; Jira, P.E.; Wevers, R.A.; Urban, Z.; Morava, E.

    2009-01-01

    AIM: Due to the occasional association pathological fractures and osteoporosis we evaluated four patients with cutis laxa syndrome for skeletal anomalies. PATIENT/METHODS: We prospectively evaluated four patients, a male and a female child and a brother-sister sib pair, with dysmorphic features, gro

  6. Hypoalbuminemia in Donors with Twin-Twin Transfusion Syndrome

    NARCIS (Netherlands)

    Verbeek, L.; Middeldorp, J. M.; Hulzebos, C. V.; Oepkes, D.; Walther, F. J.; Lopriore, E.

    2013-01-01

    Objective: To estimate the differences in albumin levels between donors and recipients with twin-twin transfusion syndrome (TTTS). Methods: We performed a matched case-control study including twin pairs with TTTS treated conservatively (conservative group) or with fetoscopic laser surgery (laser gro

  7. Swallowing a Surgeon: Toward Clinical Nanorobots.

    Science.gov (United States)

    Sim, Seunghyun; Aida, Takuzo

    2017-03-21

    In this Account, "a step toward clinical nanorobots" is proposed as one of the Holy Grails in chemistry, which could lead to a great leap in the field of biomedicines when accomplished. We review our preliminary contributions to this challenge by engineering chaperonin protein GroEL to generate de novo structures and functions.

  8. Evaluating Hydrological Response of Future Land Cover Change Scenarios in the San Pedro River (U.S./Mexico) with the Automated Geospatial Watershed Assessment (AGWA) Tool.

    Science.gov (United States)

    Long-term land-use and land cover change and their associated impacts pose critical challenges to sustaining vital hydrological ecosystem services for future generations. In this study, a methodology was developed to characterize potential hydrologic impacts from future urban gro...

  9. Patterns of International Capital Raisings

    OpenAIRE

    Gozzi, Juan Carlos; Levine, Ross; Schmukler, Sergio L.

    2008-01-01

    This paper documents several new patterns associated with firms issuing securities in foreign markets that motivate the need for and help guide future research. Besides noting that these international capital raisings grew almost four-fold from 1991 to 2005, accounting for 35 percent of all capital raised through security issuances, the paper has three main findings. First, a large and gro...

  10. United States Files Complaint Against Volkswagen, Audi and Porsche for Alleged Clean Air Act Violations

    Science.gov (United States)

    WASHINGTON - The U.S. Department of Justice, on behalf of the U.S. Environmental Protection Agency, today filed a civil complaint in federal court in Detroit, Michigan against Volkswagen AG, Audi AG, Volkswagen Group of America, Inc., Volkswagen Gro

  11. Effect of an experimental zirconia-silica coating technique on micro tensile bond strength of zirconia in different priming conditions

    NARCIS (Netherlands)

    Chen, C.; Kleverlaan, C.J.; Feilzer, A.J.

    2012-01-01

    Objectives This study aimed to evaluate the adhesive properties of a MDP-containing resin cement to a colored zirconia ceramic, using an experimental zirconia-silica coating technique with different priming conditions. Methods 18 zirconia ceramic discs (Cercon base colored) were divided into two gro

  12. Tired Children : Burnout or Chronic Fatigue Syndrome? Literature Study.

    NARCIS (Netherlands)

    Nijboer, J.M.

    2006-01-01

    This literature study is a preparation on a traineeship and master thesis research at the Northern Center for Health Care research. The topic of this traineeship and research will be burnout in children. A question from pedagogical practice to the science shop of the University Medical Centre in Gro

  13. The phase lags of high-frequency quasi-periodic oscillations in four black-hole candidates

    CERN Document Server

    Mendez, Mariano; Belloni, Tomaso; Sanna, Andrea

    2013-01-01

    We measured the phase-lag spectrum of the high-frequency quasi-periodic oscillations (QPO) in the black hole systems (at QPO frequencies) GRS 1915+105 (35 Hz and 67 Hz), GRO J1655-40 (300 Hz and 450 Hz), XTE J1550-564 (180 Hz and 280 Hz), and IGR J17091-3624 (67 Hz). The lag spectra of the 67-Hz QPO in, respectively, GRS 1915+105 and IGR J17091-3624, and the 450-Hz QPO in GRO J1655-40 are hard (hard photons lag the soft ones) and consistent with each other, with the hard lags increasing with energy. On the contrary, the lags of the 35-Hz QPO in GRS 1915+105 are soft, with the lags becoming softer as the energy increases; the lag spectrum of the 35-Hz QPO is inconsistent with that of the 67-Hz QPO. The lags of the 300-Hz QPO in GRO J1655-40, and the 180-Hz and the 280-Hz QPO in XTE J1550-564 are independent of energy, consistent with each other and with being zero or slightly positive (hard lags). For GRO J1655-40 the lag spectrum of the 300-Hz QPO differs significantly from that of the 450-Hz QPOs. The simila...

  14. Overnight levels of luteinizing hormone, follicle-stimulating hormone and growth hormone before and during gonadotropin-releasing hormone analogue treatment in short boys born small for gestational age

    NARCIS (Netherlands)

    D.C.M. van der Kaay (Danielle); F.H. de Jong (Frank); S.R. Rose (Susan); R.J.H. Odink (Roelof); W.M. Bakker-Van Waarde (Willie); E.J. Sulkers (Eric); A.C.S. Hokken-Koelega (Anita)

    2009-01-01

    textabstractAims: To evaluate if 3 months of gonadotropin-releasing hormone analogue (GnRHa) treatment results in sufficient suppression of pubertal luteinizing hormone (LH) and follicle-stimulating hormone (FSH) profile patterns in short pubertal small for gestational age (SGA) boys. To compare gro

  15. The genetics of East African populations: a Nilo-Saharan component in the African genetic landscape

    NARCIS (Netherlands)

    Dobon, B.; Hassan, H.Y.; Laayouni, H.; Luisi, P.; Ricano-Ponce, I.; Zhernakova, A.; Wijmenga, C.; Tahir, H.; Comas, D.; Netea, M.G.; Bertranpetit, J.

    2015-01-01

    East Africa is a strategic region to study human genetic diversity due to the presence of ethnically, linguistically, and geographically diverse populations. Here, we provide new insight into the genetic history of populations living in the Sudanese region of East Africa by analysing nine ethnic gro

  16. Physiological and molecular characterization of lucerne (Medicago sativa L.) germplasm with improved seedling freezing tolerance

    Science.gov (United States)

    We conducted greenhouse experiments to compare 14 alfalfa germplasms for their survival following freezing. Among these germplasms are collections adapted to the Grand River National Grasslands in South Dakota. Our hypothesis was that these collections developed tolerance to survive the frigid gro...

  17. Prebiotic effects of non-digestible oligo- and polysaccharides

    NARCIS (Netherlands)

    Hartemink, R.

    1999-01-01

    This thesis is the result of work carried out within a four-year multi-disciplined program, entitled ' Non-digestible oligosaccharides in foods and feed'. Within the project, four Ph.D. students were employed at the Food Chemistry, Food Microbiology, Human Nutrition and Animal Nutrition gro

  18. Stratigraphy of the type Maastrichtian – a synthesis

    NARCIS (Netherlands)

    Jagt, J.W.M.; Jagt-Yazykova, E.A.

    2012-01-01

    A synthesis of the stratigraphy of the Maastrichtian Stage in its extended type area, that is, southern Limburg (the Netherlands), and adjacent Belgian and German territories, is presented with a brief historical overview. Quarrying activities at the large quarry complex of ENCI-HeidelbergCement Gro

  19. Past, Present and Future Challenges To Science and Sustainability At EPA: A Review

    Science.gov (United States)

    This paper reviews critical actions taken by EPA since the 1992 Rio Earth Summit to overcome its single media approach to environmental protection and advance the concept and practice of sustainability. Sustainability is defined as both a goal and a process that link economic gro...

  20. Doelgroepsegmentatie in de primaire preventie van cardiovasculaire ziekten

    NARCIS (Netherlands)

    Kok, F.J.

    1982-01-01

    Profiles of target groups (target group segmentation) in the primary prevention of cardiovascular disease (CVD), can lead to an effective target group-directed approach in health education. Objectives in this thesis are: 1) To identify demographic and socioeconomic characteristics of such target gro

  1. Taxonomic revision of the afrotropical genus Megatrigon Johnson, 1898 (Diptera: Syrphidae)

    DEFF Research Database (Denmark)

    Doczkal, Dieter; Radenković, Snežana; Lyneborg, Leif

    2016-01-01

    The genus-group taxon Megatrigon Johnson, 1898, stat. nov., is revised and treated as a valid genus within the Merodontini (= Eumerini). Extensive diagnoses are given for the genus and for its three constituent species groups: argenteus group [11 spp.], nivalis group [monotypic], sexfasciatus gro...

  2. Plasmonic nano-antenna a-Si:H solar cell

    NARCIS (Netherlands)

    Di Vece, M.; Kuang, Y; Duren, S.; Charry, J.; van Dijk, L.; Schropp, R.E.I.

    2013-01-01

    In this work the effects of plasmonics, nano-focusing, and orthogonalization of carrier and photon pathways are simultaneously explored by measuring the photocurrents in an elongated nano-scale solar cell with a silver nanoneedle inside. The silver nanoneedles formed the support of a conformally gro

  3. Watertightness of concrete tunnel structures

    NARCIS (Netherlands)

    Glerum, A.

    1982-01-01

    The Netherlands are situated in the delta. of the rivers Rhine, Meuse and Scheldt. Therefore the ground mainly consists.of sediments, such as sand, clay and silt. In certain regions peat layers of varying thickness are found. The high permeability of some of these materials and the fact that the gro

  4. GEO 600 spitzt die Ohren. Der deutsche Gravitationswellendetektor erreicht seine geplante Empfindlichkeit

    Science.gov (United States)

    Borgeest, Ulf

    2005-04-01

    Die Physiker und Astronomen betreiben weltweit fünf große Laserinterferometer zur Messung von Gravitationswellen, darunter GEO 600 bei Hannover. Die hochempfindlichen Detektoren sollen helfen, die energiereichsten Vorgänge im Weltall aufzuklären und fundamentale physikalische Probleme zu lösen. Zur Zeit beginnen die ersten Messungen mit einer Empfindlichkeit, die astronomische Beobachtungen erlaubt.

  5. S-nitrosylation of the Mitochondrial Chaperone TRAP1 Sensitizes Hepatocellular Carcinoma Cells to Inhibitors of Succinate Dehydrogenase

    DEFF Research Database (Denmark)

    Rizza, Salvatore; Montagna, Costanza; Cardaci, Simone;

    2016-01-01

    S-nitrosoglutathione reductase (GSNOR) represents the best-documented denitrosylase implicated in regulating the levels of proteins posttranslationally modified by nitric oxide on cysteine residues by S-nitrosylation. GSNOR controls a diverse array of physiologic functions, including cellular gro...

  6. Von niederer Herkunft

    DEFF Research Database (Denmark)

    Schmidt, Kjeld

    2015-01-01

    Was man heute normalerweise unter «Computing» versteht, nämlich «Personal Computing», entwickelte sich nicht aus einer ‹Turingmaschine›, sondern als Technologie zur Erleichterung großangelegter kooperativer Arbeit. Es entstanden daraus interaktive Mensch-Maschine-Systeme, etwa Laptops und...

  7. The effects of diabetes and low blood sugar levels on driving behaviour : Comparison of diabetics and non-diabetics

    NARCIS (Netherlands)

    Martens, M.; Janssen, W.H.; Stork, A.

    2001-01-01

    Under contract with the University Medical Centre, Utrecht, TNO Human Factors has conducted research to investigate the effect of diabetes on driving behaviour. In a driving simulator experiment, patients with diabetes and non-diabetics were confronted with various trafßc situations. The subject gro

  8. ‘Charleston Scarlet’ Sweetpotato

    Science.gov (United States)

    The sweetpotato [Ipomoea batatas (L.) Lam.] cultivar, ‘Charleston Scarlet’ was developed by the U.S. Department of Agriculture, Agricultural Research Service, Charleston, SC. ‘Charleston Scarlet’ produces orange-fleshed, sweet storage roots with attractive scarlet-colored skin (periderm). Vine gro...

  9. Regieren und Parteienwettbewerb in einem nivellierten Vielparteiensystem: was erwartet die deutschen Parteien? Eine Antwort aus den Niederlanden

    NARCIS (Netherlands)

    Nijhuis, T.; Korte, K.-R.

    2010-01-01

    Das Zeitalter der großen Volksparteien scheint sich in den meisten europäischen Ländern dem Ende zu zuneigen. Obwohl in den Niederlanden die gleiche Tendenz zu beobachten ist, verläuft der Prozess der Aushöhlung der christdemokratischen und sozialdemokratischen Volksparteien dort schneller und radik

  10. General classification of maturation reaction-norm shape from size-based processes

    DEFF Research Database (Denmark)

    Christensen, Asbjørn; Andersen, Ken Haste

    2011-01-01

    Phenotypic plasticity of size at maturation is commonly described using size–age maturation reaction norms (MRNs). MRNs for age and size at maturation are analyzed and classified into three general categories related to different size scalings of growth and mortality. The underlying model for gro...

  11. Planck intermediate results: XLV. Radio spectra of northern extragalactic radio sources

    DEFF Research Database (Denmark)

    Ade, P. A R; Aghanim, N.; Aller, H. D.;

    2016-01-01

    Continuum spectra covering centimetre to submillimetre wavelengths are presented for a northern sample of 104 extragalactic radio sources, mainly active galactic nuclei, based on four-epoch Planck data. The nine Planck frequencies, from 30 to 857 GHz, are complemented by a set of simultaneous gro...

  12. How to design and evaluate interventions to improve outcomes for patients with multimorbidity

    DEFF Research Database (Denmark)

    Smith, Susan M.; Bayliss, Elizabeth A.; Mercer, Stewart W.

    2014-01-01

    Multimorbidity is a major challenge for patients and healthcare providers. The limited evidence of the effectiveness of interventions for people with multimorbidity means that there is a need for much more research and trials of potential interventions. Here we present a consensus view from a gro...

  13. The Future of Digital Music Services in Three Stereotypes; How Focus Groups of End Users See the New Business Models

    NARCIS (Netherlands)

    Bookholt, Erik; Spil, Ton A.M.; Katsma, C.P.

    2013-01-01

    “I am just a stereotype” sang Terry Hall in 1980. Ariola records took them in and made the band The Specials a world success. How will that process go in 2014? Will they put it on You Tube for free? Do they need a record company? Will they have less or more fans, earn less or more money? Focus gro

  14. Einführung in die Kernphysik

    CERN Document Server

    Friedmann, Harry

    2014-01-01

    Das Buch deckt einen sehr großen Bereich der Kernphysik ab, d.h. es werden sowohl experimentelle als auch theoretische Aspekte beleuchtet sowie Anwendungen (Kernspaltung, Kernfusion, medizinischen Anwendungen, Strahlenschutz) ausführlich behandelt. Der Aufbau folgt der historischen Entwicklung. Schließlich wird auch Basiswissen aus der Teilchenphysik kurz angesprochen.

  15. Use of GIS-based Site-specific Nitrogen Management for Improving Energy Efficiency

    Science.gov (United States)

    To our knowledge, geographical information system (GIS)-based site-specific nitrogen management (SSNM) techniques have not been used to assess agricultural energy costs and efficiency. This chapter uses SSNM case studies for corn (Zea mays L.) grown in Missouri and cotton (Gossypium hirsutum L.) gro...

  16. Rat models in obesity research : functions for PMCH and LPIN1 in energy homeostasis regulation

    NARCIS (Netherlands)

    Mul, J.D.

    2010-01-01

    The number of people that are overweight or obese has shown an alarming increase worldwide in past three decades. Good news however is that, at least in the US , this increase seems to have stalled. As mentioned in chapter 1, the obese population can be roughly divided into two groups. The first gro

  17. Fracture strength of direct versus indirect laminates with and without fiber application at the cementation interface

    NARCIS (Netherlands)

    Gresnigt, Marco M. M.; Ozcan, Mutlu

    2007-01-01

    Objectives. This study compared the fracture strength of direct and indirect resin composite laminate veneers and evaluated the effect of a bidirectional E-glass woven fiber application at different locations at the cementation interface. Methods. Standard preparations on canines (N = 50, 10 per gro

  18. Von Hippel-Lindau disease (vHL)

    DEFF Research Database (Denmark)

    Binderup, Marie Louise Mølgaard; Bisgaard, Søs Marie Luise; Harbud, Vibeke

    2013-01-01

    These clinical guidelines outline the criteria and recommendations for diagnostic and genetic work-up of families suspected of von Hippel-Lindau disease (vHL), as well as recommendations for prophylactic surveillance for vHL patients. The guideline has been composed by the Danish Coordination Gro...

  19. Geographische Revue: Zeitschrift für Literatur und Diskussion = Migration

    OpenAIRE

    2009-01-01

    Inhalt: Essays: Hartmut Esser: Ist das Konzept der Assimilation überholt? Ludger Pries: Transnationalismus, Migration und Inkorporation. Herausforderungen an Raum und Sozialwissenschaften Michael Bommes: Migration in der modernen Gesellschaft Diskussion: Christoph Scheuplein: Der Paradigmenwechsel als große Erzählung Harald Bathelt und Johannes Glückler: Plädoyer für eine relationale Wirtschaftsgeographie Einzelrezensionen

  20. Nutrition of red drum, Sciaenops ocellatus L.: An additional evaluation of the effects of soya-based diets and supplemental prebiotic

    Science.gov (United States)

    We assessed the effects of a fishmeal- (FM) and two soybean- [SOY = soybean meal (SBM) + soy protein concentrate (SPC)] based diets [with or without prebiotic (GroBiotic® -A; GBA) supplementation] on the production performance, non-specific immunity, blood parameters, and gut microbiota diversity of...

  1. Extreme flooding tolerance in Rorippa

    NARCIS (Netherlands)

    Akman, M.; Bhikharie, A.; Mustroph, A.; Sasidharan, Rashmi

    2014-01-01

    Low oxygen stress imposed by floods creates a strong selection force shaping plant ecosystems in flood-prone areas. Plants inhabiting these environments adopt various adaptations and survival strategies to cope with increasing water depths. Two Rorippa species, R. sylvestris and R. amphibia that gro

  2. Interspecific amphiploid-derived alloplasmic male sterility with defective anthers, narrow disk florets, and small ray flowers in sunflower

    Science.gov (United States)

    The cytoplasmic male-sterility (CMS)/fertility-restoration system is important for hybrid sunflower (Helianthus annuus L.) seed production. Two novel alloplasmic CMSs, designated CMS GRO1 and CMS MAX3 with defective anthers, narrow disk florets with no swollen corolla, and short, narrow ray flowers,...

  3. The Development and Evaluation of an Innovative Internet-Based Breast Cancer Psychosocial Intervention

    Science.gov (United States)

    2002-10-01

    Weinberg, Schmale, Uken, & Wessel, 1996), Sucht (King, 1994), Traumatisierung aufgrund sexueller Gewalterfahrung (Finn & Lavitt, 1994...Lebens umgesetzt werden. Georg W. Alpers Wie die oben geschilderte Initiative CHESS legen viele Angebote im Internet gro~en Wert auf die Bereitstellung...zu analysierenden Texte konnen nach einer Fehlerkorrektur als einfache Textdateien verarbeitet werden. Wir haben uns fUr die Werte der Kategorien

  4. Het arrest Residex: terugvordering moet, nietigverklaring mag

    NARCIS (Netherlands)

    Montijn, J.; Saanen, N.

    2012-01-01

    Het recente arrest Residex van het Hof van Justitie geeft inzicht in de maatregelen die de nationale rechterlijke instanties kunnen en moeten treffen indien sprake is van onwettige steun. Deze maatregelen zijn niet altijd eenvoudig in het nationale recht te passen. Ook zijn de maatregelen die op gro

  5. Translation as the Cultural-Historical Problem (Domestic Discussions 1930-1950 and the Present)

    NARCIS (Netherlands)

    Shchedrina, T. G.

    2010-01-01

    The issue of the cultural-historical relativity of translation is considered in this article. The author analyses the positions of the participants of modern discussions on problems of translation in the context of the history of discussion of translation strategies in Russia (1930-1950-ies) and gro

  6. Cell division activity during apical hook development

    NARCIS (Netherlands)

    Raz, V.; Koornneef, M.

    2001-01-01

    Growth during plant development is predominantly governed by the combined activities of cell division and cell elongation. The relative contribution of both activities controls the growth of a tissue. A fast change in growth is exhibited at the apical hypocotyl of etiolated seedlings where cells gro

  7. The musk rat (Ondatra zibethicus) as intermediate host of cestodes in the Netherlands

    NARCIS (Netherlands)

    Borgsteede, F.H.M.; Tibben, J.H.; Giessen, van der J.W.B.

    2003-01-01

    An investigation on the presence of larval cestodes in musk rats (Ondatra zibethicus) was carried out in two regions of the Netherlands (east Groningen and south Limburg) where in a earlier study foxes with Echinococcus multilocularis were found. A total of 1726 musk rats were dissected (1200 in Gro

  8. Politik und Sprachpolitik in der Lexikographie

    DEFF Research Database (Denmark)

    Bergenholtz, Henning; Tarp, Sven

    2007-01-01

    Lexikographie und Sprachpolitik haben gemeinsam, dass beide Bereiche eine lange Geschichte aufweisen können, und dass sie seit etwa 25 Jahren ein zunehmendes akademisches Interesse gewonnen haben. Die Sprachpolitik hat darüber hinaus in den letzten Jahren ein großes öffentliches Interesse gefunde...

  9. 12. Benga New.pmd

    African Journals Online (AJOL)

    chifaou.amzat

    10 août 2011 ... mondiaux s'intéressaient tous aux arts africains et négro-africains, dans les ... et social, risquait de faire passer au second plan les problèmes de ..... daté du 12 septembre 2007 portant publication de la liste des sites et.

  10. Hypothesis of demodicidosis rosacea flushing etiopathogenesis.

    Science.gov (United States)

    Robledo, Mary Ann; Orduz, Mariana

    2015-04-01

    Most of the patients with erythematotelangiectatic rosacea are characterized by flushing, oedema and telangiectasia. The etiopathogenesis of the flushing in rosacea patients is unknown. Clinically the flushing in rosacea is similar to the "Asian flushing syndrome". Most Asians have an overactive alcohol dehydrogenase (ADH) that tends to break down alcohol into acetaldehyde faster. People with "Asians flushing syndrome" have a genetic disorder with the Aldehyde Dehydrogenase 2(∗)2 (ALDH2(∗)2) allele. This is the reason why they do not metabolize very well the acetaldehyde that comes from the alcohol, which means that acetaldehyde takes much longer to clear from their blood. ALDH2 enzyme is primarily responsible for oxidation of acetaldehyde derived from ethanol metabolism, as well as oxidation of various other endogenous and exogenous aldehydes. Acetaldehyde produces the vasodilatation in the "Asian flushing syndrome". The antibodies against the GroEl chaperonin protein, a 62-kDa heat shock protein were found in the Bacillus oleronius isolated from Demodex mites, in rosacea patients. The GroEl chaperonin protein is a protein that plays a key role in normal folding of ALDH2. If the GroEl chaperonin antibodies found in patients with rosacea, cross react with the human GroEl chaperonin protein, they will not fold normally the ALDH2, and then the enzyme will not metabolize the acetaldehyde. Many of the patients with rosacea have a concomitant infection with Helicobacter pylori in their stomach. The H.pylori produces high amounts of acetaldehyde, which comes from their metabolism of ethanol or carbohydrates. As a result, high amounts of acetaldehyde will circulate for longer time in the blood, until the liver CYP2E1(p450) enzyme system finally metabilizes the acetaldehyde, during that period of time the patients will experience a flushing as well as the people with the "Asian flushing syndrome" suffer when they drink ethanol. To prove the hypothesis it is necessary

  11. Application of Nutrient Enriched Biochar to Grow Bananas at the Plantation Scale

    Science.gov (United States)

    Nzengung, Valentine

    2017-04-01

    The majority of soils in Cameroon consist of varying laterites derived from granites. The lateritic soils are generally depleted in nutrients. The most fertile soils in Cameroon are young soils that have formed from volcanic rocks of the Cameroon volcanic line (CVL). The richer volcanic soils which are found in the southwest region and the western regions are used to grow the major cash crops, including cocoa, coffee, rubber, banana, tea, and palm fruits. The government owned Cameroon Development Corporation (CDC) and private commercial farmers in the country have resorted to the heavy use of imported agrochemicals to mitigate the serious and persistent soil fertility challenges. Cameroon is the third largest biomass producer in Africa. This means that Cameroon has a high biomass production potential. Among the many types of biomass available for biochar production in Cameroon, empty fruit bunches (EFB) from the many palm oil plantations offer one of the largest concentration of biomass. CDC alone produces over 200,000 tons of EFB biomass each year. The corporation uses less than half of the EFB it produces in its palm oil processing mills for mulching. The remaining EFB are disposed by open burning leading to significant air pollution. In 2015, we entered into a collaborative understanding with CDC to dispose some of its EFB by pyrolysis to produce biochar. The produced biochar is enriched with natural plant nutrients obtained from animal waste (poultry chicken manure) and volcanic lava dust from the 2001 eruption of Mount Cameroon. The biochar, chicken litter and volcanic rock dust is aged for 14 - 21 days to produce a 100% natural soil enhancer commercialized under the name "QwikGro". The QwikGro is undergoing field evaluation on three hectares of banana plantation owned by CDC. The field trial began in June 2016. Of the three hectares, one hectare of the bananas was planted using 100% (only) QwikGro, the second hectare was planted with 50% QwikGro and received

  12. Expression of soluble, biologically active recombinant human endostatin in Escherichia coli.

    Science.gov (United States)

    Xu, Han-Mei; Zhang, Guo-Yuan; Ji, Xiao-Dan; Cao, Lin; Shu, Luan; Hua, Zi-Chun

    2005-06-01

    Endostatin, a 20kDa C-terminal fragment of collagen XVIII, is a potent anti-angiogenic protein and inhibitor of tumor growth. Recombinant endostatin was prepared from Escherichia coli deposited as insoluble, inactive inclusion bodies. In the present study, we produced soluble and biologically active recombinant human endostatin (rhEndostatin) in E. coli by employing both co-expression of the molecular chaperones and lower temperature fermentation. Two groups of chaperones Trigger factor and GroEL-GroES (GroEL/ES), DnaK-DnaJ-GrpE and GroEL/ES, were co-expressed, respectively, with rhEndostatin at different temperatures (37, 25, and 16 degrees C). It revealed that low temperature or molecular chaperones alone could enhance the production of active rhEndostatin; meanwhile, combinational employment of low temperature cultivation (16 degrees C) together with co-expression of DnaK-DnaJ-GrpE and GroEL/ES was more effective to prevent aggregation of rhEndostatin. The production of soluble rhEndostatin was about 36 mg/L, and at least 16 mg of rhEndostatin was purified from 1L flask culture. The purified rhEndostatin specifically inhibited the proliferation of endothelial cell-bovine capillary endothelial cell in a dose-dependent manner, and it showed potent anti-angiogenic capability on the chorioallantoic membrane of chick embryo in vivo. Our study provides a feasible and convenient approach to produce soluble and biologically active rhEndostatin.

  13. Chemokines influence the migration and fate of neural precursor cells from the young adult and middle-aged rat subventricular zone.

    Science.gov (United States)

    Gordon, R J; Mehrabi, N F; Maucksch, C; Connor, B

    2012-01-01

    We have previously demonstrated a role for the chemokines MCP-1, MIP-1α and GRO-α in directing subventricular zone (SVZ)-derived neural precursor cell migration towards the site of cell death in the adult rodent brain. However the influence of chemokines such as MCP-1, MIP-1α and GRO-α on the differentiation of adult neural precursor cells has not previously been investigated. Further, as the majority of neurological disorders and injuries occur during ageing, it is important to investigate the effect of chemokines on adult neural precursor cell cultures obtained from the ageing brain. This study therefore examined the effect of MCP-1, MIP-1α and GRO-α on SVZ-derived neural precursor cell differentiation in vitro, and assessed whether precursor cells from the middle-aged rat brain (13 months old) follow the same migratory and differential profile as neural precursor cells obtained from the young adult rat brain (2 months old). We observed that each of the chemokines examined generated differing effects in regards to neuronal or glial differentiation. Further, both MIP-1α and GRO-α increased total cell number, suggesting an effect on precursor cell proliferation and/or survival. In agreement with cultures obtained from young adult brains, SVZ-derived neural precursor cells cultured from the middle-aged brain exhibited chemotactic migration in response to a concentration gradient. These results indicate that the chemokines MCP-1, MIP-1α and GRO-α can influence both the migration and fate choice of SVZ-derived neural precursor cells, as well as promoting cell viability. While a response to each of these chemokines is maintained in the middle-aged brain, a distinct age-related alteration in differential fate can be identified.

  14. A comparison of anatomy-based inverse planning with simulated annealing and graphical optimization for high-dose-rate prostate brachytherapy.

    Science.gov (United States)

    Morton, Gerard C; Sankreacha, Raxa; Halina, Patrick; Loblaw, Andrew

    2008-01-01

    Dose distribution in a high-dose-rate (HDR) brachytherapy implant is optimized by adjusting source dwell positions and dwell times along the implanted catheters. Inverse planning with fast simulated annealing (IPSA) is a recently developed algorithm for anatomy-based inverse planning, capable of generating an optimized plan in less than 1min. The purpose of this study is to compare dose distributions achieved using IPSA to those obtained with a graphical optimization (GrO) algorithm for prostate HDR brachytherapy. This is a retrospective study of 63 consecutive prostate HDR brachytherapy implants planned and treated using on-screen GrO to a dose of 10Gy per implant. All plans were then recalculated using IPSA, without changing any parameters (contours, catheters, number, or location of dwell positions). The IPSA and GrO plans were compared with respect to target coverage, conformality, dose homogeneity, and normal tissue dose. The mean volume of target treated to 100% of prescription dose (V(100)) was 97.1% and 96.7%, and mean Conformal Index 0.71 and 0.68 with GrO and IPSA, respectively. IPSA plans had a higher mean homogeneity index (0.69 vs. 0.63, p<0.001) and lower volume of target receiving 150% (30.2% vs. 35.6%, p<0.001) and 200% (10.7% vs. 12.7%, p<0.001) of the prescription dose. Mean dose to urethra, rectum, and bladder were all significantly lower with IPSA (p<0.001). IPSA plans tended to be more reproducible, with smaller standard deviations for all measured parameters. Plans generated using IPSA provide similar target coverage to those obtained using GrO but with lower dose to normal structures and greater dose homogeneity.

  15. Correlation of measurable serum markers of inflammation with lung levels following bilateral femur fracture in a rat model

    Directory of Open Access Journals (Sweden)

    Benjamin W Sears

    2010-08-01

    Full Text Available Benjamin W Sears1, Dustin Volkmer1, Sherri Yong2, Ryan D Himes1, Kristen Lauing1, Michele Morgan1, Michael D Stover1, John J Callaci11Department of Orthopaedics, 2Department of Pathology, Loyola University Medical Center, Maywood, IL, USAIntroduction: Evaluation of the systemic inflammatory status following major orthopedic trauma has become an important adjunct in basing post-injury clinical decisions. In the present study, we examined the correlation of serum and lung inflammatory marker levels following bilateral femur fracture.Materials and methods: 45 Sprague Dawley rats underwent sham operation or bilateral femoral intramedullary pinning and mid-diaphyseal closed fracture via blunt guillotine. Animals were euthanized at specific time points after injury. Serum and lung tissue were collected, and 24 inflammatory markers were analyzed by immunoassay. Lung histology was evaluated by a blinded pathologist.Results: Bilateral femur fracture significantly increased serum markers of inflammation including interleukin (IL-2, IL-6, IL-10, GM-CSF, KC/GRO, MCP-1, and WBC. Femur fracture ­significantly increased serum and lung levels of IL-1a and KC/GRO at 6 hours. Lung levels of IL-6 ­demonstrated a trend towards significance. Histologic changes in pulmonary tissue after fracture included pulmonary edema and bone elements including cellular hematopoietic cells, bone fragments and marrow emboli.Discussion and conclusion: Our results indicate that bilateral femur fracture with fixation in rats results in increases in serum markers of inflammation. Among the inflammatory markers measured, rise in the serum KC/GRO (CINC-1, a homolog to human IL-8, correlated with elevated levels of lung KC/GRO. Ultimately, analysis of serum levels of KC/GRO (CINC-1, or human IL-8, may be a useful adjunct to guide clinical decisions regarding surgical timing.Keywords: blunt trauma, injury, cytokine, IL-8, bone marrow emboli

  16. Observations of Accreting Pulsars

    Science.gov (United States)

    Bildsten, Lars; Chakrabarty, Deepto; Chiu, John; Finger, Mark H.; Koh, Danny T.; Nelson, Robert W.; Prince, Thomas A.; Rubin, Bradley C.; Scott, D. Matthew; Stollberg, Mark; Vaughan, Brian A.; Wilson, Colleen A.; Wilson, Robert B.

    1997-01-01

    We summarize 5 years of continuous monitoring of accretion-powered pulsars with the Burst and Transient Source Experiment (BATSE) on the Compton Gamma Ray Observatory. Our 20-70 keV observations have determined or refined the orbital parameters of 13 binaries, discovered five new transient accreting pulsars, measured the pulsed flux history during outbursts of 12 transients (GRO J1744-28, 4U 0115+634, GRO J1750-27, GS 0834-430, 2S 1417-624, GRO J1948+32, EXO 2030+375, GRO J1008-57, A0535+26, GRO J2058+42, 4U 1145-619, and A1118-616), and also measured the accretion torque history during outbursts of six of those transients whose orbital param- eters were also known. We have also continuously measured the pulsed flux and spin frequency for eiaht persistently accreting pulsars (Her X-1, Cen X-3, Vela X-1, OAO 1657-415, GX 301-2, 4U 1626-67, 4U 1538-52, and GX 1+4). Because of their continuity and uniformity over a long baseline, BATSE observations have provided new insights into the long-term behavior of accreting magnetic neutron stars. We have found that all accreting pulsars show stochastic variations in their spin frequencies and luminosities, including those displaying secular spin-up or spin-down on long timescales, which blurs the con- ventional distinction between disk-fed and wind-fed binaries. Pulsed flux and accretion torque are strongly correlated in outbursts of transient accreting pulsars but are uncorrelated, or even anti- correlated, in persistent sources. We describe daily folded pulse profiles, frequency, and flux measurements that are available through the Compton Observatory Science Support Center at NASA/Goddard Space Flight Center.

  17. Malformaciones vasculares: claves diagnósticas para el radiólogo Vascular malformations: diagnostic clues for the radiologist

    Directory of Open Access Journals (Sweden)

    Ricardo García Mónaco

    2012-12-01

    Full Text Available Las malformaciones vasculares son enfermedades raras ocasionadas por errores en el desarrollo embriológico de los vasos sanguíneos. Estas lesiones, junto con los tumores vasculares, constituyen las anomalías vasculares. En el caso de las malformaciones vasculares, éstas se denominan de acuerdo al vaso que conforma la lesión y se clasifican según las características del flujo en malformaciones de bajo o alto flujo. Si bien la mayor parte son diagnosticadas según criterios clínicos, algunas lesiones requieren de estudios complementarios tanto para el establecimiento de un correcto diagnóstico como para la evaluación de su extensión. La ecografía Doppler suele ser el estudio inicial para conocer las características hemodinámicas y contribuir al diagnóstico diferencial entre un tumor y una malformación vascular. La resonancia magnética (RM es el mejor método para evaluar la extensión de las lesiones y su relación con estructuras adyacentes. El diagnóstico preciso del tipo de malformación vascular es importante por su pronóstico y tratamiento diferentes. La Radiología Intervencionista constituye uno de los pilares fundamentales en el manejo terapéutico. Es altamente recomendable un abordaje multidisciplinario dada la complejidad de estas lesiones, particularmente en la elección de las opciones terapéuticas y en el seguimiento clínico de los pacientes.Vascular malformations are rare conditions resulting from the abnormal development of blood vessels. These lesions, together with vascular tumors, represent the vascular anomalies. Vascular malformations are named according to which type of vessel is affected and are classified into high- or low-flow malformations on the basis of flow characteristics. Most vascular malformations are diagnosed according to clinical criteria. However, some require imaging studies to confirm the diagnosis or assess its extension. Doppler ultrasound is the usual initial study for hemodynamic assessment and for its contribution to differential diagnosis between vascular tumors and malformations. Magnetic resonance imaging (MRI is the best technique for evaluating the extent of the lesions and their relationship with adjacent structures. An accurate diagnosis of the type of vascular malformation is of importance because of the different prognosis and treatment. Interventional radiology is one of the cornerstones in the therapeutic management. A multidisciplinary approach is highly recommended given the complexity of these lesions, particularly for selecting therapeutic options and for the patient clinical follow-up.

  18. THE CHAPTERS ON GRAMMAR IN THE “ALFAVITAR RADI UČENIJA MALYCH DETEJ”: STRUCTURE, CONTENTS, SOURCES

    Directory of Open Access Journals (Sweden)

    I. A. Podtergera

    2015-01-01

    Full Text Available The paper is devoted to a 17th century primer whose author is currently considered to be Evfimij Čudovskij. It provides a detailed analysis of the structure and content of the first sections of that part of the book which is dedicated to grammar, commenting on the way in which the author revised the grammar textbooks that he used as source material. The analysis demonstrates that he was an experienced educator who had a professional knowledge of didactics and knew the principles that the school curriculum was based on. It also becomes clear that the author was acquainted with the contemporary West European theories of grammar. All this casts doubt on the presumed authorship of Evfimij Čudovskij, who received no systematic school education.Further linguistic and textual analysis shows that Aldus Manutius’ Latin commentary on the Greek alphabet De literis Graecis, ac diphthongis, et quemadmodum ad nos ueniant, which accompanied the editions of Constantine Laskaris’ Greek grammar, was used as a source. In 1687 the Lichudes brothers compiled an abridged version of Laskaris’ grammar offering their own explanations of certain linguistic phenomena and whole sentences of these are reproduced nearly verbatim in the primer. The paper concludes by arguing that the conception of this primer belonged to the Lichudes, that it was conceived as they were studying Old Church Slavonic using Smotryckyj’s grammar, possibly with the help of Evfimij Čudovskij. I suggest that Evfimij was a source of the Slavic data for the book, but not its author.

  19. Određivanje buke radi sertifikacije mlaznih aviona / Procedures in the noise certification of jet aircraft

    Directory of Open Access Journals (Sweden)

    Gordana Jurin

    2005-01-01

    Full Text Available U radu su prikazane osnovne teorijske postavke i matematički modeli koji su značajni za obradu akustičkih i neakustičkih podataka u procesu određivanja buke mlaznih aviona prema međunarodnom standardu ICAO, Annex 16, za potrebe sertiflkacije. Proces obuhvata i postupak eliminacije efekata ambijentalnog šuma iz signala avionske buke. Kao primer, data je praktična primena navedenog postupka na rezultate merenja za avion G-2A. / This paper presents a basic theoretical scheme and a mathematical model that is used to calculate jet aircraft noise level for certification purposes in accordance with the international standard ICAO Annex 16. In this process acoustic and nonacoustic data are used. The process also includes the procedure for eliminating ambient noise effects out of the aircraft noise signal. This procedure was applied in the case of noise measurement results for the G-2Ajet aircraft.

  20. The chemokine CXCL1/growth related oncogene increases sodium currents and neuronal excitability in small diameter sensory neurons

    Directory of Open Access Journals (Sweden)

    Wick Dayna M

    2008-09-01

    Full Text Available Abstract Background Altered Na+ channel expression, enhanced excitability, and spontaneous activity occur in nerve-injury and inflammatory models of pathological pain, through poorly understood mechanisms. The cytokine GRO/KC (growth related oncogene; CXCL1 shows strong, rapid upregulation in dorsal root ganglion in both nerve injury and inflammatory models. Neurons and glia express its receptor (CXCR2. CXCL1 has well-known effects on immune cells, but little is known about its direct effects on neurons. Results We report that GRO/KC incubation (1.5 nM, overnight caused marked upregulation of Na+ currents in acutely isolated small diameter rat (adult sensory neurons in vitro. In both IB4-positive and IB4-negative sensory neurons, TTX-resistant and TTX-sensitive currents increased 2- to 4 fold, without altered voltage dependence or kinetic changes. These effects required long exposures, and were completely blocked by co-incubation with protein synthesis inhibitor cycloheximide. Amplification of cDNA from the neuronal cultures showed that 3 Na channel isoforms were predominant both before and after GRO/KC treatment (Nav 1.1, 1.7, and 1.8. TTX-sensitive isoforms 1.1 and 1.7 significantly increased 2 – 3 fold after GRO/KC incubation, while 1.8 showed a trend towards increased expression. Current clamp experiments showed that GRO/KC caused a marked increase in excitability, including resting potential depolarization, decreased rheobase, and lower action potential threshold. Neurons acquired a striking ability to fire repetitively; IB4-positive cells also showed marked broadening of action potentials. Immunohistochemical labelling confirmed that the CXCR2 receptor was present in most neurons both in dissociated cells and in DRG sections, as previously shown for neurons in the CNS. Conclusion Many studies on the role of chemokines in pain conditions have focused on their rapid and indirect effects on neurons, via release of inflammatory mediators

  1. Apparent total tract energy and macronutrient digestibility of one- to three-day-old, adult ground, extruded, and canned chicken-based diets in domestic cats (Felis silvestris catus).

    Science.gov (United States)

    Kerr, K R; Morris, C L; Burke, S L; Swanson, K S

    2014-08-01

    There has been a recent increase in the popularity of feeding unconventional diets, including whole prey diets, to domestic cats (Felis silvestris catus). Data are needed that allow animal caretakers to choose and formulate diets that meet the nutritional requirements of their cats. Our objective was to evaluate the effects of feeding 1- to 3-d-old whole chicks (WHO), ground adult chicken product (GRO), a chicken-based canned diet (CAN), and a chicken-based extruded diet (EXT) on apparent total tract energy and macronutrient digestibility, N balance, and blood metabolites of domestic cats (n = 11). Macronutrient, energy, and moisture concentrations of diets varied greatly (e.g., CP: 35 to 72% DM); however, cats fed all diets maintained BW and N balance. In general, cats fed WHO had lower nutrient digestibility than those fed CAN and EXT. Cats fed GRO had greater nutrient digestibility than cats fed commercial diets. For example, apparent OM and GE digestibility coefficients were greater (P ≤ 0.05) for cats fed CAN (86 and 88%, respectively), EXT (88 and 88%), and GRO (94 and 95%) compared with those fed WHO (83 and 83%) and greater (P ≤ 0.05) for cats fed GRO compared with those fed CAN and EXT. Many blood metabolites were modified by diet, but most remained within reference ranges for domestic cats. Serum cholesterol was elevated above the reference range for all treatments and greater (P ≤ 0.05) for cats fed WHO compared with those fed CAN, EXT, and GRO. Serum creatinine concentrations were above the reference range for all treatments and greater (P ≤ 0.05) for cats fed GRO compared with those fed CAN or WHO. These data indicate that the whole prey tested herein maintained short-term health and are adequately digestible for use in companion animal diets. Research is needed to determine the global and long-term health implications of feeding whole or ground diets to domestic cats, which may be different in terms of macronutrient, energy, and moisture

  2. Sterile medfly males of the tsl Vienna 8 genetic sexing strain display improved mating performance with ginger root oil

    Energy Technology Data Exchange (ETDEWEB)

    Paranhos, Beatriz Jordao; Alves, Renata Morelli, E-mail: bjordao@cpatsa.embrapa.b [EMBRAPA Semi-Arido, Petrolina, PE (Brazil); McInnis, Donald [U.S. Department of Agriculture (USDA/ARS/PBARC), Honolulu, HI (United States). Agricultural Research Service. Pacific Basin Agricultural Research Center; Uramoto, Keiko [Universidade de Sao Paulo (USP), SP (Brazil); Damasceno, Itala; Malavasi, Aldo [Biofabrica Moscamed Brasil, Juazeiro, BA (Brazil); Goncalves, Nilmara [Valexport, Petrolina, PE (Brazil); Costa, Maria de Lourdes; Walder, Julio [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil); Nascimento, Antonio [EMBRAPA Mandioca e Fruticultura, Cruz das Almas, BA (Brazil)

    2006-07-01

    A key point of the sterile insect technique applied to the medfly, Ceratitis capitata, is that the sterile males produced in the laboratory should have at least a minimal sexual compatibility with wild females. Among several genetic sexing tsl (Temperature Sensitive Lethal) strains of C. capitata mass-reared around the world, the Biofabrica Moscamed Brasil has chosen the most recent mass produced tsl strain, Vienna 8 (V8), which has been evaluated in the San Francisco River Valley, Brazil, since April, 2005. The tests were accomplished in field cages, with different treatments for V8 males, sterile or fertile, exposed to the aroma of ginger root oil (GRO) or not, versus wild males and females. Males of one strain (V8 or wild) were painted white on the thorax the day before the mating tests. All the insects were virgin, and early in the morning (7-8 A.M.) males were released inside the field cages, 10 min. before females. Mating pairs were collected in glass vials, until early afternoon. From this raw data, both the type of male mating and the time in copula were recorded for each pair. Then, the total percentage of mated females, the RSI (Relative Sterility Index), and Fried's competitiveness values (C), were calculated for each field cage. The percentage of females mated was statistically higher to sterile males exposed to GRO than to non exposed to GRO. Time in copula was significantly higher for wild flies than for laboratory flies, except for the case of fertile V8 males exposed to GRO x wild females. The RSI and C values were significantly higher for V8 males (irradiated and fertile) treated with GRO than for V8 males not treated with GRO. The results indicate that there is adequate sexual compatibility between sterile males of the tsl Vienna 8 strain and wild C. capitata females from the San Francisco River Valley, Brazil. Also, the radiation dose of 95 Gy, used to sterilize the males, did not affect their sexual activity. Ginger root oil acted as a

  3. Uncertainty Comparison of Visual Sensing in Adverse Weather Conditions

    Directory of Open Access Journals (Sweden)

    Shi-Wei Lo

    2016-07-01

    Full Text Available This paper focuses on flood-region detection using monitoring images. However, adverse weather affects the outcome of image segmentation methods. In this paper, we present an experimental comparison of an outdoor visual sensing system using region-growing methods with two different growing rules—namely, GrowCut and RegGro. For each growing rule, several tests on adverse weather and lens-stained scenes were performed, taking into account and analyzing different weather conditions with the outdoor visual sensing system. The influence of several weather conditions was analyzed, highlighting their effect on the outdoor visual sensing system with different growing rules. Furthermore, experimental errors and uncertainties obtained with the growing rules were compared. The segmentation accuracy of flood regions yielded by the GrowCut, RegGro, and hybrid methods was 75%, 85%, and 87.7%, respectively.

  4. Topoisomerase activity during the heat shock response in Escherichia coli K-12.

    Science.gov (United States)

    Camacho-Carranza, R; Membrillo-Hernández, J; Ramírez-Santos, J; Castro-Dorantes, J; Chagoya de Sánchez, V; Gómez-Eichelmann, M C

    1995-01-01

    During the upshift of temperature from 30 to 42, 45, 47, or 50 degrees C, an increase in the level of supercoiling of a reporter plasmid was observed. This increase was present in groE and dnaK mutants but was inhibited in cells treated with chloramphenicol and novobiocin. The intracellular [ATP]/[ADP] ratio increased rapidly after an upshift in temperature from 30 to 47 degrees C and then decreased to reach a level above that observed at 30 degrees C. These results suggest that gyrase and proteins synthesized during heat shock are responsible for the changes seen in plasmid supercoiling. Proteins GroE and DnaK are probably not involved in this phenomenon. PMID:7768879

  5. Testing gravity of a regular and slowly rotating phantom black hole by quasi-periodic oscillations

    Science.gov (United States)

    Chen, Songbai; Wang, Mei; Jing, Jiliang

    2016-10-01

    We extend firstly the regular phantom black hole solution to a slowly rotating black hole case and find that the phantom field depresses the angular velocity of the event horizon and suppresses the super-radiation of the black hole. We also probe the dependence of quasi-periodic oscillations frequencies in a relativistic precession model on the phantom parameter. With the observation data of GRO J1655-40, we make a constraint on the parameters of the regular and slowly rotating phantom black hole. Our results show that although the best-fit value of the phantom parameter b is small, the allowed value of b in the 1σ region is b\\lt 0.619, which means that the phantom theoretical model cannot be excluded by the constraint from quasi-periodic oscillations with the observation data of GRO J1655-40.

  6. Role for macrophage inflammatory protein-2 in lipopolysaccharide-induced lung injury in rats

    DEFF Research Database (Denmark)

    Schmal, H; Shanley, T P; Jones, M L;

    1996-01-01

    Macrophage inflammatory protein-2 (MIP-2) is a C-X-C chemokine that possesses chemotactic activity for neutrophils. Rat MIP-2 was cloned and expressed as a 7.9-kDa peptide that exhibited dose-dependent neutrophil chemotactic activity at concentrations from 10 to 250 nM. Rabbit polyclonal Ab...... to the 7.9-kDa peptide showed reactivity by western blot analysis and suppressed its in vitro chemotactic activity. Cross-desensitization chemotaxis experiments suggested that the chemotactic responses elicited by MIP-2 and the related chemokine, cytokine-induced neutrophil chemoattractant, may be mediated...... through a common receptor. Also, chemotactic responses to human GRO-alpha were blocked by exposure of human neutrophils to either GRO-alpha or rat MIP-2, suggesting conservation of this receptor-mediated response. After LPS instillation into rat lung, mRNA for MIP-2 was up-regulated in a time...

  7. Efficient expression and purification of biologically active human cystatin proteins.

    Science.gov (United States)

    Chauhan, Sakshi; Tomar, Raghuvir S

    2016-02-01

    Cystatins are reversible cysteine protease inhibitor proteins. They are known to play important roles in controlling cathepsins, neurodegenerative disease, and in immune system regulation. Production of recombinant cystatin proteins is important for biochemical and function characterization. In this study, we cloned and expressed human stefin A, stefin B and cystatin C in Escherichia coli. Human stefin A, stefin B and cystatin C were purified from soluble fraction. For cystatin C, we used various chaperone plasmids to make cystatin C soluble, as it is reported to localize in inclusion bodies. Trigger factor, GroES-GroEL, DnaK-DnaJ-GrpE chaperones lead to the presence of cystatin C in the soluble fraction. Immobilized metal affinity chromatography, glutathione sepharose and anion exchange chromatography techniques were employed for efficient purification of these proteins. Their biological activities were tested by inhibition assays against cathepsin L and H3 protease.

  8. The Comptel project and Logica's involvement

    Science.gov (United States)

    McCormack, A.; Bains, N.; Jenkins, C.; Hall, R.; Jeanes, A.

    1991-01-01

    The scientific objectives of the application software development of the Compton Imaging Telescope (Comptel) project is described and Logica's specific involvement is detailed with respect to scientific, technical, and managerial aspects. Comptel is one of four instruments onboard the Gamma Ray Observatory (GRO), a NASA project, to be launched in March 1991. It will study gamma ray characteristics of point sources, diffuse emission from the galaxy, cosmic diffusion flux and broadened line emissions. It will also determine the direction of arrival as well as the energy of gamma ray photons from 1 to 30 MeV. Logica has played an active part in the writing of software requirements for the subsystems of GRO and subsequently the detailed design, code, test, and integration of these subsystems. Logica's roles include the development of a module calibration subsystem which includes the development of gamma ray burst software and pulsar analysis software both of which are discussed in detail.

  9. Chaperonin Structure - The Large Multi-Subunit Protein Complex

    Directory of Open Access Journals (Sweden)

    Irena Roterman

    2009-03-01

    Full Text Available The multi sub-unit protein structure representing the chaperonins group is analyzed with respect to its hydrophobicity distribution. The proteins of this group assist protein folding supported by ATP. The specific axial symmetry GroEL structure (two rings of seven units stacked back to back - 524 aa each and the GroES (single ring of seven units - 97 aa each polypeptide chains are analyzed using the hydrophobicity distribution expressed as excess/deficiency all over the molecule to search for structure-to-function relationships. The empirically observed distribution of hydrophobic residues is confronted with the theoretical one representing the idealized hydrophobic core with hydrophilic residues exposure on the surface. The observed discrepancy between these two distributions seems to be aim-oriented, determining the structure-to-function relation. The hydrophobic force field structure generated by the chaperonin capsule is presented. Its possible influence on substrate folding is suggested.

  10. İpek Çalışlar: Mrs. Atatürk. Berlin: Orlanda Verlag 2008

    Directory of Open Access Journals (Sweden)

    Hans-Peter Laqueur

    2009-07-01

    Full Text Available Die 2006 erschienene Biographie über Latife Uşşaki, die geschiedene Ehefrau Mustafa Kemal Atatürks, erregte in der Türkei große Aufmerksamkeit. Das hochgelobte Buch rekonstruierte anhand zahlreicher Fakten die Ehe zweier starker Persönlichkeiten, die nach kurzer Zeit zerbrach. Die stark gekürzte deutsche Übersetzung ist sowohl aufgrund diverser formeller als auch inhaltlicher Defizite nur mit großen Einschränkungen zu empfehlen.The 2006 biography of Latife Uşşaki, the divorced wife of Mustafa Kemal Atatürk, incited much attention in Turkey. The highly praised book reconstructed the short-lived marriage of two strong personalities on the basis of numerous facts. The strongly abridged German translation can only be recommended with great reservation, for it exhibits many deficits with regard to form and content.

  11. Die Radziwiłł-Karte Polen-Litauens von 1613

    Directory of Open Access Journals (Sweden)

    Christian Schumacer

    2015-12-01

    Full Text Available Am Beispiel einer Karte des Großfürstentums Litauen zeigt Christian Schumacher, inwieweit kartografische Darstellungsweisen die Wahrnehmung derselben beeinflussen. Die 1585 vom litauischen Magnat Krzysztof »Sierotka« Radziwiłł in Auftrag gegebene Karte wird aus der Perspektive der Kritischen Kartographie und der Historischen Kulturwissenschaft betrachtet und mittels interdisziplinär angelegten Methoden untersucht. Sein Ergebnis ist klar: „Insgesamt sind die Ereignisse, Darstellungen, Verhältnisse und räumlichen Konzepte so gewählt, dass die Wahrnehmung des Betrachters zu einem katholischen, militärisch und wirtschaftlich potenten und autonomen Großfürstentum Litauen gelenkt wird.“ – Die Frage, ob diese Deutung den historischen Gegebenheiten entsprach, kann durch die Lektüre seiner Bachelorarbeit „Die Radziwiłł-Karte Polen-Litauens von 1613“ ebenfalls beantwortet werden.

  12. Die transvesikale Prostataadenomenukleation – heute noch zeitgemäß?

    Directory of Open Access Journals (Sweden)

    Atanassov G

    2014-01-01

    Full Text Available Die offene Prostatektomie stellt die älteste operative Methode zur Therapie der benignen Prostatahyperplasie (BPH dar. Die chirurgische Therapie großer Adenome rückt in neuerer Zeit wieder in den Fokus, da bei Patienten mit leichten Symptomen der unteren Harnwege (LUTS die orale Medikation zu erhöhten Inzidenzraten von großen Prostataadenomen führt. Seit Mitte der 1990er-Jahre etabliert sich zunehmend die Holmium-Laser-Enukleation der Prostata, kurz HoLEP. Eine Literaturrecherche und der Vergleich mit einer retrospektiven Analyse von 80 Patienten mit offener Adenomenukleation eines Krankenhauses der Grund- und Regelversorgung soll Aufschluss darüber geben, ob die offene Operationsvariante heute noch zeitgemäß ist.

  13. Synthesis of silver nanoparticles in an aqueous suspension of graphene oxide sheets and its antimicrobial activity.

    Science.gov (United States)

    Das, Manash R; Sarma, Rupak K; Saikia, Ratul; Kale, Vinayak S; Shelke, Manjusha V; Sengupta, Pinaki

    2011-03-01

    A solution-based approach to the synthesis of silver (Ag) nanoparticles by chemical reduction of AgNO(3) in a graphene oxide (GrO) suspension is demonstrated. X-ray diffraction and transmission electron microscopy indicate that the Ag nanoparticles, of size range 5-25nm, were decorated on the GrO sheets. The size and shape of the Ag nanoparticles are dependent on the concentration of the AgNO(3) solution. Antimicrobial activity of such hybrids materials is investigated against the Gram negative bacteria Escherichia coli and Pseudomonous aeruginosa. The bacterial growth kinetics was monitored in nutrient broth supplemented with the Ag nanoparticle-GrO suspension at different conditions. It was observed that P. aeruginosa is comparatively more sensitive to the Ag nanoparticle-GrO suspension. 2010 Elsevier B.V. All rights reserved.

  14. Testing gravity of a regular and slowly rotating phantom black hole by quasi-periodic oscillations

    CERN Document Server

    Chen, Songbai; Jing, Jiliang

    2016-01-01

    We extend firstly the regular phantom black hole solution to a slowly rotating black hole case and find that the phantom field depresses the angular velocity of the event horizon and suppresses the super-radiation of black hole. We also probe the dependence of quasi-periodic oscillations frequencies in relativistic precession model on the phantom parameter. With the observation data of GRO J1655-40, we make a constraint on the parameters of the regular and slowly rotating phantom black hole. Our results show that although the best-fit value of the phantom parameter $b$ is small, the allowed value of $b$ in the $1\\sigma$ region is $b<0.619$, which means that the phantom theoretical model can not be excluded by the constraint from quasi-periodic oscillations with the observation data of GRO J1655-40.

  15. Rezension von: Thomas Martinec, Claudia Nitschke (Hg.: Familie und Identität in der deutschen Literatur. Frankfurt am Main u.a.: Peter Lang Verlag 2009. — Ariane Eichenberg: Familie – Ich – Nation. Göttingen: V&R unipress 2009.

    Directory of Open Access Journals (Sweden)

    Christine Kanz

    2010-06-01

    Full Text Available Die Themen Generation und Familie gehören zu den wichtigsten Sujets literarischer Texte, insbesondere wenn diese die Herausbildung oder die Zerstörung von Identität problematisieren. Ein neuer, von Claudia Nitschke und Thomas Martinec herausgegebener Tagungsband ist der literatur- und kulturwissenschaftlichen Auseinandersetzung mit diesen Themen gewidmet. Sechs Jahrzehnte nach dem Ende des Zweiten Weltkrieges hat vor allem das Genre des Generationenromans Hochkonjunktur, insbesondere Texte der dritten Generation zum Umgang der Großeltern-Generation mit dem Nationalsozialismus. Den Verfasser/-innen dieses neuen, in sich heterogenen Genres wird in der Studie von Ariane Eichenberg allerdings keine allzu große Wirkmächtigkeit bescheinigt. Im Gegenteil nährten die modischen Texte mit den Funden aus der Vergangenheit vor allem das Ich der Erzähler/-innen, deren eigene Gefühle und Selbstvergewisserung in einer von Unsicherheiten geprägten Gegenwart.

  16. Expression and Purification of ST14, a Tumor Metastasis-associated Protein, and Its Activity Assay%肿瘤转移相关蛋白ST14的表达、纯化及活性鉴定

    Institute of Scientific and Technical Information of China (English)

    葛维挺; 郑树; 孙立峰; 史影; 胡涵光; 丁克峰

    2004-01-01

    ST14 is one of the type Ⅱ transmembrane serine proteases that correlates with the process of tumor metastasis. The C-terminal catalytic region (900 bp) of ST14 was cloned into the expression vector pGEX-4T-2 and the positive plasmid pGEX-4T-2-STI4 was transformed into E. coli BL21, then cultured and induced with IPTG. The chaperonin GroEL was found to be tightly associated with the fusion protein and co-purified with it by regular GST affinity chromatography. A method for the removal of contaminating GroEL from GST-ST14 fusion protein was described, the purity of product was 96.2%. Enzyme activity assay indicated that this fusion protein had serine protease activity.

  17. Auf den Schultern von Riesen und Zwergen Einsteins unvollendete Revolution

    CERN Document Server

    Renn, Jürgen

    2006-01-01

    Dies ist die Geschichte von Einsteins unvollendeter Revolution, einer tiefgreifenden Veränderung unserer Begriffe von Raum, Zeit, Materie und Strahlung. Diese Revolution begann in Einsteins Wunderjahr 1905, wurde durch seine allgemeine Relativitätstheorie aus dem Jahre 1915 fortgesetzt und wirkt in den heutigen Versuchen der Wissenschaft, die Entstehung und das Schicksal des Universums zu verstehen, weiter. Vor dem Hintergrund einer historischen Theorie des wissenschaftlichen Fortschritts wird Einsteins bis heute nicht abgeschlossene Revolution als das Ergebnis einer langfristigen Entwicklung des Wissens verständlich. Anhand der spannenden Geschichte von Einsteins Entdeckungen wird nachvollziehbar, warum große Denker wie Einstein weiter gesehen haben als ihre Vorgänger. Sie standen nicht nur auf den Schultern von Riesen, also den wissenschaftlichen Leistungen einzelner großer Vorgänger wie Newton, sondern auch auf den Schultern von "Zwergen", dem wissenschaftlichen Wissen, dem technischen Wissen, und d...

  18. 26 S proteasomes function as stable entities

    DEFF Research Database (Denmark)

    Hendil, Klavs B; Hartmann-Petersen, Rasmus; Tanaka, Keiji

    2002-01-01

    Most proteins in eukaryotic cells are degraded by 26-S proteasomes, usually after being conjugated to ubiquitin. In the absence of ATP, 26-S proteasomes fall apart into their two sub-complexes, 20-S proteasomes and PA700, which reassemble upon addition of ATP. Conceivably, 26-S proteasomes...... dissociate and reassemble during initiation of protein degradation in a ternary complex with the substrate, as in the dissociation-reassembly cycles found for ribosomes and the chaperonin GroEL/GroES. Here we followed disassembly and assembly of 26-S proteasomes in cell extracts as the exchange of PA700...... subunits between mouse and human 26-S proteasomes. Compared to the rate of proteolysis in the same extract, the disassembly-reassembly cycle was much too slow to present an obligatory step in a degradation cycle. It has been suggested that subunit S5a (Mcb1, Rpn10), which binds poly-ubiquitin substrates...

  19. Efficient expression of Human papillomavirus 16 E7 oncoprotein fused to C-terminus of Tobacco mosaic virus (TMV) coat protein using molecular chaperones in Escherichia coli.

    Science.gov (United States)

    Folwarczna, Jitka; Moravec, Tomas; Plchova, Helena; Hoffmeisterova, Hana; Cerovska, Noemi

    2012-09-01

    The Human papillomavirus 16 (HPV16) E7 oncoprotein is a promising candidate for development of anti-cancer therapeutic vaccine. We have prepared the expression construct carrying mutagenized E7 oncoprotein fused to the C-terminus of Tobacco mosaic virus (TMV) coat protein via 15 amino acids β-sheet linker. The fusion protein was expressed in Escherichia coli MC 1061 cells. We have obtained high level expression, but most of the protein remained in insoluble inclusion bodies. To increase the ratio of soluble protein various molecular chaperones (TF, DnaK-DnaJ-GrpE, GroEL-GroES) were used. The immunological reactivity of expressed recombinant protein was evaluated with anti-E7 and anti-TMV antibodies. The distribution of expressed product during ultracentrifugation on sucrose gradient was studied. Copyright © 2012 Elsevier Inc. All rights reserved.

  20. (Keine Strategie für Medizinbibliotheken? [(No strategy for medical libraries?

    Directory of Open Access Journals (Sweden)

    Obst, Oliver

    2013-09-01

    Full Text Available [english] Strategy is a big word. Which medical library does have a logical, let alone sustainable strategy? The following article is trying to break down this big word on a tradable level, to make it usable for everyday practice. In the sea of information, libraries and their areas of expertise could be small, agile speedboats compared to the overpowering ocean liners.[german] Strategie ist ein großes Wort. Welche Medizinbibliothek hat schon eine logische, geschweige denn nachhaltige Strategie aufzuweisen? Im folgenden Artikel wird versucht, dieses große Wort auf ein handelbares Level herunter zu brechen, um es für die alltägliche Praxis nutzbar zu machen. Bibliotheken und deren Spezialgebiete können als kleine, wendige Schnellboote auf dem Meer der Information durchaus den übermächtigen Ozeandampfern Konkurrenz machen.

  1. New records of the rare glacial relict Eurytemora lacustris (Poppe 1887 (Copepoda; Calanoida in atypical lake habitats of northern Germany

    Directory of Open Access Journals (Sweden)

    Peter KASPRZAK

    2011-02-01

    Full Text Available During monitoring investigations of lakes in Schleswig-Holstein (northern Germany in 2000 and 2008, the calanoid copepod Eurytemora lacustris (POPPE 1887 was found in three lakes of the Ratzeburger Lake complex: the Kleine Küchensee, the Große Küchensee and the Große Ratzeburger See. The species has a broad geographic distribution but has become rare and endangered by eutrophication and global change. The lakes are mesotrophic (Gr. Ratzeburger See and eutrophic (Kl. Küchensee, Gr. Küchensee with cool (1 mg O2 L-1. Food and physical conditions seem to be favourable enough to permit survival and reproduction of the species at least in spring. It remains unclear, however, whether the populations in the studied lakes are autochthonous. Eurytemora lacustris possibly invaded the lakes or was transported via a canal from the Schaalsee upstream, where a viable population exists.

  2. Functional analysis of High-Throughput data for dynamic modeling in eukaryotic systems

    OpenAIRE

    Flöttmann, Max

    2013-01-01

    Das Verhalten Biologischer Systeme wird durch eine Vielzahl regulatorischer Prozesse beeinflusst, die sich auf verschiedenen Ebenen abspielen. Die Forschung an diesen Regulationen hat stark von den großen Mengen von Hochdurchsatzdaten profitiert, die in den letzten Jahren verfügbar wurden. Um diese Daten zu interpretieren und neue Erkenntnisse aus ihnen zu gewinnen, hat sich die mathematische Modellierung als hilfreich erwiesen. Allerdings müssen die Daten vor der Integration in Modelle agg...

  3. Vom Kataster in die Fahrsimulation: OpenDRIVE aus Geodaten

    OpenAIRE

    Scholz, Michael

    2014-01-01

    Bei der Entwicklung von Fahrerassistenzsystemen spielt die Simulation eine große Rolle. Urbane Szenarien spielen immer eine wichtigere Rolle, vor allem im Kontext zukünftiger Car2X-Kommunikation. Im Projekt "Virtuelle Welt" wurde eine Werkzeugkette zur automatischen Generierung von logischen Straßenbeschreibungen aus weiträumig verfügbaren Geodaten (Katasterdaten, OSM, Navteq) entwickelt. Dieses Poster beschreibt die grundlegenden Prozessschritte bis hin zu einer 3D-Representation, welche zus...

  4. Important role of class I heat shock genes hrcA and dnaK in the heat shock response and the response to pH and NaCl stress of group I Clostridium botulinum strain ATCC 3502.

    Science.gov (United States)

    Selby, Katja; Lindström, Miia; Somervuo, Panu; Heap, John T; Minton, Nigel P; Korkeala, Hannu

    2011-05-01

    Class I heat shock genes (HSGs) code for molecular chaperones which play a major role in the bacterial response to sudden increases of environmental temperature by assisting protein folding. Quantitative reverse transcriptase real-time PCR gene expression analysis of the food-borne pathogen Clostridium botulinum grown at 37°C showed that the class I HSGs grpE, dnaK, dnaJ, groEL, and groES and their repressor, hrcA, were expressed at constant levels in the exponential and transitional growth phases, whereas strong downregulation of all six genes was observed during stationary phase. After heat shock from 37 to 45°C, all HSGs were transiently upregulated. A mutant with insertionally inactivated hrcA expressed higher levels of class I HSGs during exponential growth than the wild type, followed by upregulation of only groES and groES after heat shock. Inactivation of hrcA or of dnaK encoding a major chaperone resulted in lower maximum growth temperatures than for the wild type and reduced growth rates under optimal conditions compared to the wild type. The dnaK mutant showed growth inhibition under all tested temperature, pH, and NaCl stress conditions. In contrast, the growth of an hrcA mutant was unaffected by mild temperature or acid stress compared to the wild-type strain, indicating that induced class I HSGs support growth under moderately nonoptimal conditions. We show that the expression of class I HSGs plays a major role for survival and growth of C. botulinum under the stressful environmental conditions that may be encountered during food processing or growth in food products, in the mammalian intestine, or in wounds.

  5. CULTURAL TOURISM MOTIVATION – THE CASE OF ROMANIAN YOUTHS

    OpenAIRE

    Adina Negrusa; Medet Yolal

    2012-01-01

    Cultural attractions have become particularly important and play an important role in tourism at all levels. They are also increasingly being placed at the centre of urban and rural development and constitute an important aspect of social and cultural lives of the residents. Cultural tourism is defined as ‘visits by persons from outside the host community motivated wholly or in part by interest in the historical, artistic, scientific or lifestyle/heritage offerings of a community, region, gro...

  6. Hacia un modelo de análisis de políticas públicas operativo. un enfoque basado en los actores, sus recursos y las instituciones

    OpenAIRE

    Knoepfel, Peter; Larrue, Corinne; Varone, Frédéric; Hinojosa Dieck, Miriam

    2010-01-01

    Towards an operative analysis of public policies: An approach focused on actors, resources and institutions. This article develops an analytical model which is centred on the individual and collective behaviour of actors involved during different stages of public policy. We postulate that the content and institutional characteristics of public action (dependent variable) are the result of interactions between political-administrative authorities, on the one hand, and, on the other, social gro...

  7. START-UP AND GROWTH OF IMMIGRANT SMALL BUSINESSES: THE IMPACT OF SOCIAL AND HUMAN CAPITAL

    OpenAIRE

    JENNIFER M. SEQUEIRA; Abdul A Rasheed

    2006-01-01

    Networks, and their resulting social capital, can be key determinants of successful business start-up for immigrant entrepreneurs. Historically, immigrants have settled in communities characterized by networks that consist of strong ties. Network theory suggests that in addition to strong ties, success also requires the development of weak ties. In this paper, we develop a model of the relationships between strong and weak ties, and the likelihood of a business start-up and its subsequent gro...

  8. Jeschke, Lebrecht; Lenschow, Uwe; Zimmermann, Horst (Red.): Die Naturschutzgebiete in Mecklenburg-Vorpommern

    OpenAIRE

    Funkel, Christiane

    2014-01-01

    Mecklenburg-Vorpommern ist eines der reichsten Bundesländer Deutschlands, reich an einmaligen Landschaften mit einer großartigen, in weiten Teilen noch gut erhaltenen Naturausstattung. Namen wie Dars, Hiddensee, Schaalsee oder Muritz sprechen für sich. Daraus erwächst nahezu die Verpflichtung, diese wertvollen Landschaften, die überwiegend nach Naturschutzrecht geschützt sind, in einer Übersicht vorzustellen. Das nun nach mehreren Jahren Bearbeitungszeit vorliegende Werk beeindruckt auf den „...

  9. Kinderarmut. Über ihre Bedeutung für Bildungsprozesse Heranwachsender und die Grenzen der Pädagogik

    OpenAIRE

    Liesner, Andrea; Wischmann, Anke

    2010-01-01

    Die Verfasserinnen setzen sich mit den Fragen auseinander, was Armut für die von ihr Betroffenen bedeutet, was die Pädagogik zur Verbesserung ihrer Situation beitragen kann und welche Herausforderung Armut für eine erziehungswissenschaftliche Forschung bedeutet, die sich für die heterogenen gesellschaftlichen Bedingungen von Bildung und Erziehung interessiert. Die empirische Basis der Untersuchung bildet Kinderarmut in Hamburg, in einer der reichsten Großstädte Deutschlands. Die Verfasserinne...

  10. Are Private Firms Really More Tax Aggressive Than Public Firms ?

    OpenAIRE

    Pierk, Jochen

    2016-01-01

    This paper tests the notion that private firms are more tax aggressive than public firms. Tax avoidance measures, e.g. effective tax rates, cannot be used to compare private and public firms when private and public firms have different levels of importance on financial accounting earnings (Hanlon and Heitzman 2010). To disentangle financial reporting incentives from tax aggressiveness, I use the fact that European groups must prepare two sets of financial statements: first, gro...

  11. Der Neokortex der Säugetiere : Evolution und Funktion

    OpenAIRE

    Kern, Alexander

    2012-01-01

    In der Neurobiologie nimmt die Untersuchung der Großhirnrinde (Neokortex) eine gewisse Sonderstellung ein, weil das Verständnis dieser hierarchisch übergeordneten Region für die Analyse bzw. Rekonstruktion der Hirnfunktionen insgesamt von entscheidender Bedeutung ist. Dabei macht Folgendes die bedeutungsvolle Stellung des Neokortex aus: seine späte stammesgeschichtliche und ontogenetische Entwicklung, welche bei mehreren Säugetiergruppen mit einer ungewöhnlichen Massenzunahme und Plastizit...

  12. PENERAPAN METODE ANALISIS VIDEO SOFTWARE TRACKER DALAM PEMBELAJARAN FISIKA KONSEP GERAK JATUH BEBAS UNTUK MENINGKATKAN KETERAMPILAN PROSES SISWA KELAS X SMAN 1 SOOKO MOJOKERTO

    OpenAIRE

    Muhammad Habibbulloh; Madlazim

    2014-01-01

    Has been done research for knowing skills process which owned by students after applying method video analysis software Tracker in grade X SMAN 1 Sooko Mojokerto. Purpose of this research was describe enforceability of learning, skills science process which owned students after applying method of video analysis software Tracker, students activity , the response of students, and constraints which faced when learning process. Type of this research is Descriptive Quantitative with design one gro...

  13. Wuchsdepressionen von Gehölzarten im Baumschulbereich

    OpenAIRE

    Spethmann, Wolfgang; Wilstermann, Maike

    2003-01-01

    Im Rahmen des Projektes wurden wissenschaftliche und populäre Veröffentlichungen zum Themenbereich Wachstumsdepressionen, Nachbauprobleme, Bodenmüdigkeit und Allelopathie recherchiert und ausgewertet. Es wurden Praktiker, Berater und Wissenschaftler befragt und die jeweiligen Ergebnisse in 3 Workshops diskutiert. Rosaceae sind von den Nachbauproblemen am stärksten betroffen. Die größte wirtschaftliche Bedeutung haben dabei Rose und Apfel. Mit großer Sicherheit kann ein Zusammenhang zwischen e...

  14. Die familiäre Bewältigung einer Krebserkrankung im Kindes- und Jugendalter - Möglichkeiten der standardisierten Erfassung mit Selbstbeurteilungsverfahren: Ergebnisse einer empirischen Vorstudie

    OpenAIRE

    Goldbeck, Lutz

    1998-01-01

    In einer schriftlichen Befragung gaben 44 Eltern pädiatrisch-onkologischer Patienten während der Therapiephase Auskunft über ihre eigene Krankheitsbewältigung sowie über die Krankheitsbewältigung und die körperlichen Beschwerden ihrer erkrankten Kinder. Die große Mehrzahl der untersuchten Familien zeigte sich aus Elternsicht den mit der Krankheit und Behandlung verbundenen Belastungen gewachsen. Aktive, problemorientierte Bewältigungssstrategien in Verbindung mit einer optimistischen Grundein...

  15. JobTIPS: A Transition to Employment Program for Individuals with Autism Spectrum Disorders

    OpenAIRE

    Strickland, Dorothy C.; Coles, Claire D.; Southern, Louise B.

    2013-01-01

    This study evaluated the effectiveness of an Internet accessed training program that included Theory of Mind-based guidance, video models, visual supports, and virtual reality practice sessions in teaching appropriate job interview skills to individuals with high functioning ASD. In a randomized study, twenty two youth, ages 16 to 19, were evaluated during two employment interviews. Half received a training intervention following the initial interview and the half who served as a contrast gro...

  16. Effects of continuous midwifery labour support for women with severe fear of childbirth

    OpenAIRE

    Sydsjö, Gunilla; Blomberg, Marie; Palmquist, Sofie; Angerbjörn, Louise; Bladh, Marie; Josefsson, Ann

    2015-01-01

    Background: Continuous support by a midwife during childbirth has shown positive effects on the duration of active labour, use of pain relief and frequency of caesarean section (CS) in women without fear of childbirth (FOC). We have evaluated how continuous support by a specially assigned midwife during childbirth affects birth outcome and the subjective experience of women with severe FOC. Methods: A case-control pilot study with an index group of 14 women with severe FOC and a reference gro...

  17. Psychological intervention reduces self-reported performance anxiety in high school music students

    OpenAIRE

    Braden, Alice M.; Margaret Sarah Osborne; Wilson, Sarah J.

    2015-01-01

    Music performance anxiety (MPA) can be distressing for many young people studying music, and may negatively impact upon their ability to cope with the demands and stressors of music education. It can also lead young people to give up music or to develop unhealthy coping habits in their adult music careers. Minimal research has examined the effectiveness of psychological programs to address MPA in young musicians. Sixty-two adolescents were pseudo-randomised to a cognitive behavioural (CB) gro...

  18. Erfolgsfaktoren des virtuellen Settings in der psychologischen Internet-Beratung

    Directory of Open Access Journals (Sweden)

    Nils Günther Schulze

    2007-04-01

    Full Text Available Es werden einige besondere Wirkfaktoren von Internet-Beratung, die gegenüber der Face-to-face-Beratung Vorteile darstellen, aus den Erfahrungen eines mehrjährigen Großprojekts zur Internet-Beratung aufgezeigt. Insbesondere werden begünstigende internetbedingte Settingeigenschaften beschrieben, und die für Prozess und Ergebnisse der psychologischen Beratung vorteilhaften psychischen Effekte identifiziert, die diese auf Seiten von Klient und Berater hervorrufen.

  19. Psychological intervention reduces self-reported performance anxiety in high school music students

    OpenAIRE

    Braden, Alice M.; Margaret Sarah Osborne; Sarah J Wilson

    2015-01-01

    Music performance anxiety (MPA) can be distressing for many young people studying music, and may negatively impact upon their ability to cope with the demands and stressors of music education. It can also lead young people to give up music or to develop unhealthy coping habits in their adult music careers. Minimal research has examined the effectiveness of psychological programs to address MPA in young musicians. Sixty-two adolescents were pseudo-randomised to a cognitive behavioural (CB) gro...

  20. Anti-Stigma Projekte in der Psychiatrie

    OpenAIRE

    2006-01-01

    Die Stigmatisierung psychiatrischer Patienten und die daraus entstehenden negativen Folgen für Früherkennung, Behandlung und Compliance sind ein großes Problem für die Betroffenen und für das gesamte psychiatrische Versorgungssystem. Die World Psychiatric Association hat deshalb weltweit Anti-Stigma Projekte ins Leben gerufen und auch andere Institutionen versuchen auf verschiedenste Weise, die Stigmatisierung und Diskriminierung psychisch Kranker zu bekämpfen. Ziel der vorliegenden Arbeit wa...