**Figure 2.**

**Metabolome-Wide Association Study (MWAS) of plasma metabolites correlated with age.** (**A**) Type 1 Manhattan plot showing -log_{10} *p* for correlation of each metabolite plotted by *m/z* (mass-to-charge ratio) and type 2 Manhattan plot showing -log_{10} *p* for correlation of each metabolite plotted by chromatographic retention time (RT) in seconds, as separated the C18 column. Plots are shown with significance (n = 1505, *p* = 0.05) and false discovery rate (n= 140, FDR = 0.2) thresholds by dashed lines, and the detailed information of metabolic features is provided in **B**) Plot of acylcarnitine correlation strength and direction (Spearman ρ) by –Log10 *p*. Acylcarnitines with *p* < 0.05 are labeled by the chain length, saturation and modification of the acyl group (see *p* = 0.05) and false discovery rate (n= 4, FDR = 0.2) thresholds by dashed lines In all plots, significant negative correlations are shown in blue, and significant positive correlations are shown in red.