Alumina matrix ceramic-nickel composites formed by centrifugal slip casting

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Justyna Zygmuntowicz

2015-12-01

Full Text Available The paper is focused on the possibility of fabricating the alumina matrix ceramic-nickel composites with gradient concentration of metal particles. Centrifugal slip casting method was chosen for the composite fabrication. This method allows fabrication of the graded distribution of nickel particles in the hollow cylinder composites. The horizontal rotation axis was applied. The samples were characterized by XRD, SEM and quantitative description of the microstructure. The macroscopic as well as SEM observations of the prepared composites confirmed the gradient concentration of Ni particles in the composite materials. The application of the centrifugal slip casting method allows for the graded distribution of metal particles in the samples.

Orientation of Al3Ti platelets in Al-Al3Ti functionally graded material manufactured by centrifugal method

International Nuclear Information System (INIS)

Watanabe, Y.; Fukui, Y.

1997-01-01

Al-Al 3 Ti functionally graded materials (FGMs) were manufactured by the centrifugal method with a commercial ingot of Al-5 mass% Ti master alloy. The alloy was melted at a liquid/solid coexisting temperature, at which Al 3 Ti remains as a solid, and then it was cast into a thick-walled ring. It was found that the Al-Al 3 Ti functionally graded material can be successfully fabricated by the centrifugal method. It was also found that the volume fraction of the Al 3 Ti can be increased by repetition of the centrifugal method. Since the shape of Al 3 Ti particles in a commercial alloy ingot is that of a platelet, the Al 3 Ti particles are arranged with their platelet planes nearly perpendicular to the radial direction. The orientation effects become stronger when the G number becomes larger. Although the final centrifugal casting was conducted under a very large centrifugal force for the specimen cast three times, the orientation effects were weaker than those in the specimen cast one time. From these observations, it is concluded that the origin of orientation of Al 3 Ti platelets can be attributed to the angular velocity gradient of the melt along the radial direction produced by the difference in the viscosity. (orig.)

A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections

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Moen, Scott T.; Hatcher, Christopher L.; Singh, Anup K.

2016-01-01

We present a miniaturized centrifugal platform that uses density centrifugation for separation and analysis of biological components in small volume samples (~5 μL). We demonstrate the ability to enrich leukocytes for on-disk visualization via microscopy, as well as recovery of viable cells from each of the gradient partitions. In addition, we simplified the traditional Modified Wright-Giemsa staining by decreasing the time, volume, and expertise involved in the procedure. From a whole blood sample, we were able to extract 95.15% of leukocytes while excluding 99.8% of red blood cells. This platform has great potential in both medical diagnostics and research applications as it offers a simpler, automated, and inexpensive method for biological sample separation, analysis, and downstream culturing. PMID:27054764

Quasi‐steady centrifuge method for unsaturated hydraulic properties

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Caputo, Maria C.; Nimmo, John R.

2005-01-01

We have developed the quasi‐steady centrifuge (QSC) method as a variation of the steady state centrifuge method that can be implemented simply and inexpensively with greater versatility in terms of sample size and other features. It achieves these advantages by somewhat relaxing the criterion for steadiness of flow through the sample. This compromise entails an increase in measurement uncertainty but to a degree that is tolerable in most applications. We have tested this new approach with an easily constructed apparatus to establish a quasi‐steady flow of water in unsaturated porous rock samples spinning in a centrifuge, obtaining measurements of unsaturated hydraulic conductivity and water retention that agree with results of other methods. The QSC method is adaptable to essentially any centrifuge suitable for hydrogeologic applications, over a wide range of sizes and operating speeds. The simplified apparatus and greater adaptability of this method expands the potential for exploring situations that are common in nature but have been the subject of few laboratory investigations.

Quasi-steady centrifuge method for unsaturated hydraulic properties

Science.gov (United States)

Caputo, Maria C.; Nimmo, John R.

2005-11-01

We have developed the quasi-steady centrifuge (QSC) method as a variation of the steady state centrifuge method that can be implemented simply and inexpensively with greater versatility in terms of sample size and other features. It achieves these advantages by somewhat relaxing the criterion for steadiness of flow through the sample. This compromise entails an increase in measurement uncertainty but to a degree that is tolerable in most applications. We have tested this new approach with an easily constructed apparatus to establish a quasi-steady flow of water in unsaturated porous rock samples spinning in a centrifuge, obtaining measurements of unsaturated hydraulic conductivity and water retention that agree with results of other methods. The QSC method is adaptable to essentially any centrifuge suitable for hydrogeologic applications, over a wide range of sizes and operating speeds. The simplified apparatus and greater adaptability of this method expands the potential for exploring situations that are common in nature but have been the subject of few laboratory investigations.

Processing of semen by density gradient centrifugation selects spermatozoa with longer telomeres for assisted reproduction techniques.

Science.gov (United States)

Yang, Qingling; Zhang, Nan; Zhao, Feifei; Zhao, Wanli; Dai, Shanjun; Liu, Jinhao; Bukhari, Ihtisham; Xin, Hang; Niu, Wenbing; Sun, Yingpu

2015-07-01

The ends of eukaryotic chromosomes contain specialized chromatin structures called telomeres, the length of which plays a key role in early human embryonic development. Although the effect of sperm preparation techniques on major sperm characteristics, such as concentration, motility and morphology have been previously documented, the possible status of telomere length and its relation with sperm preparation techniques is not well-known for humans. The aim of this study was to investigate the role of density gradient centrifugation in the selection of spermatozoa with longer telomeres for use in assisted reproduction techniques in 105 samples before and after sperm processing. After density gradient centrifugation, the average telomere length of the sperm was significantly longer (6.51 ± 2.54 versus 5.16 ± 2.29, P average motile sperm rate was significantly higher (77.9 ± 11.8 versus 44.6 ± 11.2, P average DNA fragmentation rate was significantly lower (11.1 ± 5.9 versus 25.9 ± 12.9, P sperm count (rs = 0.58; P sperm with longer telomeres. Copyright © 2015 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

Single-step fabrication of quantum funnels via centrifugal colloidal casting of nanoparticle films

Science.gov (United States)

Kim, Jin Young; Adinolfi, Valerio; Sutherland, Brandon R.; Voznyy, Oleksandr; Kwon, S. Joon; Kim, Tae Wu; Kim, Jeongho; Ihee, Hyotcherl; Kemp, Kyle; Adachi, Michael; Yuan, Mingjian; Kramer, Illan; Zhitomirsky, David; Hoogland, Sjoerd; Sargent, Edward H.

2015-01-01

Centrifugal casting of composites and ceramics has been widely employed to improve the mechanical and thermal properties of functional materials. This powerful method has yet to be deployed in the context of nanoparticles—yet size–effect tuning of quantum dots is among their most distinctive and application-relevant features. Here we report the first gradient nanoparticle films to be constructed in a single step. By creating a stable colloid of nanoparticles that are capped with electronic-conduction-compatible ligands we were able to leverage centrifugal casting for thin-films devices. This new method, termed centrifugal colloidal casting, is demonstrated to form films in a bandgap-ordered manner with efficient carrier funnelling towards the lowest energy layer. We constructed the first quantum-gradient photodiode to be formed in a single deposition step and, as a result of the gradient-enhanced electric field, experimentally measured the highest normalized detectivity of any colloidal quantum dot photodetector. PMID:26165185

Single-step fabrication of quantum funnels via centrifugal colloidal casting of nanoparticle films.

KAUST Repository

Kim, Jin Young; Adinolfi, Valerio; Sutherland, Brandon R; Voznyy, Oleksandr; Kwon, S Joon; Kim, Tae Wu; Kim, Jeongho; Ihee, Hyotcherl; Kemp, Kyle; Adachi, Michael; Yuan, Mingjian; Kramer, Illan; Zhitomirsky, David; Hoogland, Sjoerd; Sargent, Edward H

2015-01-01

Centrifugal casting of composites and ceramics has been widely employed to improve the mechanical and thermal properties of functional materials. This powerful method has yet to be deployed in the context of nanoparticles--yet size-effect tuning of quantum dots is among their most distinctive and application-relevant features. Here we report the first gradient nanoparticle films to be constructed in a single step. By creating a stable colloid of nanoparticles that are capped with electronic-conduction-compatible ligands we were able to leverage centrifugal casting for thin-films devices. This new method, termed centrifugal colloidal casting, is demonstrated to form films in a bandgap-ordered manner with efficient carrier funnelling towards the lowest energy layer. We constructed the first quantum-gradient photodiode to be formed in a single deposition step and, as a result of the gradient-enhanced electric field, experimentally measured the highest normalized detectivity of any colloidal quantum dot photodetector.

Single-step fabrication of quantum funnels via centrifugal colloidal casting of nanoparticle films.

KAUST Repository

Kim, Jin Young

2015-07-13

Centrifugal casting of composites and ceramics has been widely employed to improve the mechanical and thermal properties of functional materials. This powerful method has yet to be deployed in the context of nanoparticles--yet size-effect tuning of quantum dots is among their most distinctive and application-relevant features. Here we report the first gradient nanoparticle films to be constructed in a single step. By creating a stable colloid of nanoparticles that are capped with electronic-conduction-compatible ligands we were able to leverage centrifugal casting for thin-films devices. This new method, termed centrifugal colloidal casting, is demonstrated to form films in a bandgap-ordered manner with efficient carrier funnelling towards the lowest energy layer. We constructed the first quantum-gradient photodiode to be formed in a single deposition step and, as a result of the gradient-enhanced electric field, experimentally measured the highest normalized detectivity of any colloidal quantum dot photodetector.

Fabrication of Al/Diamond Particles Functionally Graded Materials by Centrifugal Sintered-Casting Method

International Nuclear Information System (INIS)

Watanabe, Yoshimi; Shibuya, Masafumi; Sato, Hisashi

2013-01-01

The continuous graded structure of functionally graded materials (FGMs) can be created under a centrifugal force. Centrifugal sintered-casting (CSC) method, proposed by the authors, is one of the fabrication methods of FGM under centrifugal force. This method is a combination of the centrifugal sintering method and centrifugal casting method. In this study, Al/diamond particle FGM was fabricated by the proposed method.

Centrifugal precipitation chromatography

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Ito, Yoichiro; Lin, Qi

2009-01-01

Centrifugal precipitation chromatography separates analytes according their solubility in ammonium sulfate (AS) solution and other precipitants. The separation column is made from a pair of long spiral channels partitioned with a semipermeable membrane. In a typical separation, concentrated ammonium sulfate is eluted through one channel while water is eluted through the other channel in the opposite direction. The countercurrent process forms an exponential AS concentration gradient through the water channel. Consequently, protein samples injected into the water channel is subjected to a steadily increasing AS concentration and at the critical AS concentration they are precipitated and deposited in the channel bed by the centrifugal force. Then the chromatographic separation is started by gradually reducing the AS concentration in the AS channel which lowers the AS gradient concentration in the water channel. This results in dissolution of deposited proteins which are again precipitated at an advanced critical point as they move through the channel. Consequently, proteins repeat precipitation and dissolution through a long channel and finally eluted out from the column in the order of their solubility in the AS solution. The present method has been successfully applied to a number of analytes including human serum proteins, recombinant ketosteroid isomerase, carotenoid cleavage enzymes, plasmid DNA, polysaccharide, polymerized pigments, PEG-protein conjugates, etc. The method is capable to single out the target species of proteins by affinity ligand or immunoaffinity separation. PMID:19541553

Surface Hardening of Composite Material by the Centrifugal-Casting Method

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Eidelman, E. D.; Durnev, M. A.

2018-04-01

The effect of rotation flow emerging under centrifugal casting on the first-order phase transition, i.e., crystallization, has been studied using the example of producing a gradient composite material of AK12 aluminum alloy in a mixture with basalt fibers. It has been shown that a material with a hardened surface can be created. Distribution of admixtures in the main material when there is macroscopic motion has been found.

Experimental study on enriching 12C by centrifuge method

International Nuclear Information System (INIS)

Xiao Huaxian

1994-07-01

The diamond made from the highly enriched 12 C, whose thermal conductivity and electric insulativity are much better than that of natural diamond, has widely uses in new and high technology. In many enriching 12 C methods, the gas centrifuge method is superior to others. After selecting the appropriate process gas and solving key problems, such as feed and extract, the separation experiments are performed by a single stage of centrifuge. To increase the separation capacity of single machine, various parameters in the centrifugal separation are optimized, and appropriate mechanical drive, thermal drive, hold-up and process parameters are selected. The optimal operating condition of single machine is also obtained in the cascade. Thus, highly enriched 12 C is produced in the centrifuge cascade

Comparative validation using quantitative real-time PCR (qPCR and conventional PCR of bovine semen centrifuged in continuous density gradient

Directory of Open Access Journals (Sweden)

M.V. Resende

2011-06-01

Full Text Available The objective of the present study was to determine the sperm enrichment with X-bearing spermatozoa, after one centrifugation in a Percoll or OptiPrep continuous density gradient, using quantitative real-time polymerase chain reaction (qPCR of sperm DNA and resultant in vitro-produced bovine embryos by PCR. Frozen/thawed sperm was layered on density gradients and the tubes were centrifuged. Supernatants were gently aspirated and the sperm recovered from the bottom of the tubes. Cleavage and blastocyst rates were determined through in vitro production of embryos and PCR was performed to identify the embryos' genetic sex. A difference in blastocyst rate was found in the Percoll treatment compared to OptiPrep (P<0.05. The percentage of female embryos in the Percoll and OptiPrep groups was 62.0% and 47.1%, respectively. These results were confirmed by qPCR of spermatozoa DNA and underestimation was seen only in the Percoll group. It was possible to sexing sperm using simple approach.

Microwave assisted centrifuge and related methods

Science.gov (United States)

Meikrantz, David H [Idaho Falls, ID

2010-08-17

Centrifuge samples may be exposed to microwave energy to heat the samples during centrifugation and to promote separation of the different components or constituents of the samples using a centrifuge device configured for generating microwave energy and directing the microwave energy at a sample located in the centrifuge.

Evolutionary Optimization of Centrifugal Nozzles for Organic Vapours

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Persico, Giacomo

2017-03-01

This paper discusses the shape-optimization of non-conventional centrifugal turbine nozzles for Organic Rankine Cycle applications. The optimal aerodynamic design is supported by the use of a non-intrusive, gradient-free technique specifically developed for shape optimization of turbomachinery profiles. The method is constructed as a combination of a geometrical parametrization technique based on B-Splines, a high-fidelity and experimentally validated Computational Fluid Dynamic solver, and a surrogate-based evolutionary algorithm. The non-ideal gas behaviour featuring the flow of organic fluids in the cascades of interest is introduced via a look-up-table approach, which is rigorously applied throughout the whole optimization process. Two transonic centrifugal nozzles are considered, featuring very different loading and radial extension. The use of a systematic and automatic design method to such a non-conventional configuration highlights the character of centrifugal cascades; the blades require a specific and non-trivial definition of the shape, especially in the rear part, to avoid the onset of shock waves. It is shown that the optimization acts in similar way for the two cascades, identifying an optimal curvature of the blade that both provides a relevant increase of cascade performance and a reduction of downstream gradients.

[Effect of Parasep® feces centrifuge tube method on detecting schistosome eggs].

Science.gov (United States)

Ma, Nian; Zhang, Hua-ming; Liu, Xiong; Xiao, Chuan-yun; Wen, Xiao-hong; Li, Xia; Dong, Li-chun; Cui, Cai-xia; Tu, Zu-wu

2014-08-01

To evaluate the effect of the Parasep® feces centrifuge tube method on detecting schistosome eggs. A total of 803 residents aged from 6-65 years were selected in 2 schistosomiasis endemic villages, Jiangling County, Hubei Province, and their stool samples were collected and detected parallelly by the Kato-Katz technique, nylon silk egg hatching method, and Parasep® feces centrifuge tube method at the same time. Among the 803 people, 15 cases were found of schistosome egg positive, and the positive rate was 1.87%. The positive rates of the Kato-Katz technique, nylon silk egg hatching method, and Parasep® feces centrifuge tube method were 0.75%, 1.49% and 1.12%, respectively. The schistosome eggs got with the Parasep® feces centrifuge tube method were clear and easy to identify. In low endemic areas of schistosomiasis, the Parasep® feces centrifuge tube method can be used as schistosomiasis japonica etiology diagnosis method.

Review of noise reduction methods for centrifugal fans

Science.gov (United States)

Neise, W.

1981-11-01

Several methods for the reduction of centrifugal fan noise are presented, the most of which are aimed at a lower blade passage frequency level. The methods are grouped into five categories: casing modifications to increase the distance between impeller and cutoff, the introduction of a phase shift of the source pressure fluctuations, impeller modifications, radial clearance between impeller eye and inlet nozzle, and acoustical measures. Resonators mounted at the cutoff of centrifugal fans appear to be a highly efficient and simple means of reducing the blade passage tone, and the method can be used for new fan construction and existing installations without affecting the aerodynamic performance of the fan.

Separative performance transients in a gas centrifuge

International Nuclear Information System (INIS)

Olander, D.R.

1979-01-01

A general method has been developed to calculate the behavior of the exit compositions from a gas centrifuge under unsteady conditions. The method utilizes the basic enrichment gradient equations derived by Cohen, which, in this case, contain time derivatives of the partial 235 U inventories. These partial differential equations are converted to ordinary differential equations by a linear approximation to the axial concentration distribution for use in the inventory terms only. With this simplification, analytical solution is possible for the feed concentration transient. The transient driven by a change in the feed flow rate, however, requires numerical solution. For analysis of ideal cascades in the unsteady state, the transient flow and separation characteristics of the centrifuge must be combined with total uranium and 235 U material balances on each stage

The standard centrifuge method accurately measures vulnerability curves of long-vesselled olive stems.

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Hacke, Uwe G; Venturas, Martin D; MacKinnon, Evan D; Jacobsen, Anna L; Sperry, John S; Pratt, R Brandon

2015-01-01

The standard centrifuge method has been frequently used to measure vulnerability to xylem cavitation. This method has recently been questioned. It was hypothesized that open vessels lead to exponential vulnerability curves, which were thought to be indicative of measurement artifact. We tested this hypothesis in stems of olive (Olea europea) because its long vessels were recently claimed to produce a centrifuge artifact. We evaluated three predictions that followed from the open vessel artifact hypothesis: shorter stems, with more open vessels, would be more vulnerable than longer stems; standard centrifuge-based curves would be more vulnerable than dehydration-based curves; and open vessels would cause an exponential shape of centrifuge-based curves. Experimental evidence did not support these predictions. Centrifuge curves did not vary when the proportion of open vessels was altered. Centrifuge and dehydration curves were similar. At highly negative xylem pressure, centrifuge-based curves slightly overestimated vulnerability compared to the dehydration curve. This divergence was eliminated by centrifuging each stem only once. The standard centrifuge method produced accurate curves of samples containing open vessels, supporting the validity of this technique and confirming its utility in understanding plant hydraulics. Seven recommendations for avoiding artefacts and standardizing vulnerability curve methodology are provided. © 2014 The Authors. New Phytologist © 2014 New Phytologist Trust.

Method and refrigerants for replacing existing refrigerants in centrifugal compressors

International Nuclear Information System (INIS)

Kopko, W.L.

1991-01-01

This patent describes a method for replacing an existing refrigerant in a centrifugal compressor. It comprises selecting a desired impeller Mach number for the centrifugal compressor; selecting a base refrigerant constituent; combining at least one additive refrigerant constituent with the base refrigerant constituent to form a replacement refrigerant having at least one physical or chemical property different from the existing refrigerant and substantially providing the desired impeller Mach number in the centrifugal compressor; and replacing the existing refrigerant with the replacement refrigerant

Proliferative and phenotypical characteristics of human adipose tissue-derived stem cells: comparison of Ficoll gradient centrifugation and red blood cell lysis buffer treatment purification methods.

Science.gov (United States)

Najar, Mehdi; Rodrigues, Robim M; Buyl, Karolien; Branson, Steven; Vanhaecke, Tamara; Lagneaux, Laurence; Rogiers, Vera; De Kock, Joery

2014-09-01

Adult human subcutaneous adipose tissue harbors a multipotent stem cell population, the so-called human adipose tissue-derived mesenchymal stromal cells (AT-MSCs). These cells are able to differentiate in vitro into various cell types and possess immunomodulatory features. Yet procedures to obtain AT-MSCs can vary significantly. The two most extensively used AT-MSC purification techniques are (i) density gradient centrifugation using Ficoll and (ii) red blood cell (RBC) lysis buffer treatment of the stromal vascular fraction. In the context of potential clinical cell therapy, the stem cell yield after purification and upon consecutive passages, as well as the purity of the obtained cell population, are of utmost importance. We investigated the expansion capacity and purity of AT-MSCs purified by both procedures immediately after isolation and upon consecutive passages. We also investigated possible purification-dependent differences in their expression of immune-inhibitory factors and cell adhesion molecules. We found that RBC lysis buffer treatment is a more robust and easier method to purify AT-MSCs than density gradient fractionation. However, the resulting AT-MSC-RBC population contains a significantly higher number of CD34(+) cells, particularly during the first passages after plating. From passage 4 onward, no significant differences could be observed between both populations with respect to the immunophenotype, expansion capacity and expression of immune inhibitory factors and cell adhesion molecules. Our data show that RBC lysis buffer treatment may be a good alternative to density fractionation, providing a faster, more robust and easier method to purify AT-MSCs with biologically preserved characteristics. Copyright © 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.

Capitalizing Resolving Power of Density Gradient Ultracentrifugation by Freezing and Precisely Slicing Centrifuged Solution: Enabling Identification of Complex Proteins from Mitochondria by Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Directory of Open Access Journals (Sweden)

Haiqing Yu

2016-01-01

Full Text Available Density gradient centrifugation is widely utilized for various high purity sample preparations, and density gradient ultracentrifugation (DGU is often used for more resolution-demanding purification of organelles and protein complexes. Accurately locating different isopycnic layers and precisely extracting solutions from these layers play a critical role in achieving high-resolution DGU separations. In this technique note, we develop a DGU procedure by freezing the solution rapidly (but gently after centrifugation to fix the resolved layers and by slicing the frozen solution to fractionate the sample. Because the thickness of each slice can be controlled to be as thin as 10 micrometers, we retain virtually all the resolution produced by DGU. To demonstrate the effectiveness of this method, we fractionate complex V from HeLa mitochondria using a conventional technique and this freezing-slicing (F-S method. The comparison indicates that our F-S method can reduce complex V layer thicknesses by ~40%. After fractionation, we analyze complex V proteins directly on a matrix assisted laser desorption/ionization, time-of-flight mass spectrometer. Twelve out of fifteen subunits of complex V are positively identified. Our method provides a practical protocol to identify proteins from complexes, which is useful to investigate biomolecular complexes and pathways in various conditions and cell types.

Instability of a Vacuum Arc Centrifuge

International Nuclear Information System (INIS)

Hole, M.J.; Dallaqua, R.S.; Bosco, E. del; Simpson, S.W.

2003-01-01

Ever since conception of the Vacuum Arc Centrifuge (VAC) in 1980, periodic fluctuations in the ion saturation current and floating potential have been observed in Langmuir probe measurements in the rotation region of a VAC. Our theoretical and experimental research suggests that these fluctuations are in fact a pressure-gradient driven drift mode. In this work, we summarise the properties of a theoretical model describing the range of instabilities in the VAC plasma column, present theoretical predictions and compare with detailed experiments conducted on the PCEN centrifuge at the Brazilian National Space Research Institute (INPE). We conclude that the observed instability is a 'universal' instability, driven by the density-gradient, in a plasma with finite conductivity

Design Method for Channel Diffusers of Centrifugal Compressors

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Mykola Kalinkevych

2013-01-01

Full Text Available The design method for channel diffusers of centrifugal compressors, which is based on the solving of the inverse problem of gas dynamics, is presented in the paper. The concept of the design is to provide high pressure recovery of the diffuser by assuming the preseparation condition of the boundary layer along one of the channel surfaces. The channel diffuser was designed with the use of developed method to replace the vaned diffuser of the centrifugal compressor model stage. The numerical simulation of the diffusers was implemented by means of CFD software. Obtained gas dynamic characteristics of the designed diffuser were compared to the base vaned diffuser of the compressor stage.

Vitality of oligozoospermic semen samples is improved by both swim-up and density gradient centrifugation before cryopreservation.

Science.gov (United States)

Counsel, Madeleine; Bellinge, Rhys; Burton, Peter

2004-05-01

To ascertain whether washing sperm from oligozoospermic and normozoospermic samples before cryopreservation improves post-thaw vitality. Normozoospermic (n = 18) and oligozoospermic (n = 16) samples were divided into three aliquots. The first aliquot remained untreated and the second and third aliquots were subjected to the swim-up and discontinuous density gradient sperm washing techniques respectively. Vitality staining was performed, samples mixed with cryopreservation media and frozen. Spermatozoa were thawed, stained, and vitality quantified and expressed as the percentage of live spermatozoa present. Post-thaw vitality in untreated aliquots from normozoospermic samples (24.9% +/- 2.3; mean +/- SEM) was significantly higher (unpaired t-tests; P vitality was significantly higher after swim-up in normozoospermic samples (35.6% +/- 2.1; P vitality in oligozoospermic (22.4% +/- 1.0; P vitality in cryopreserved oligozoospermic samples was improved by both the swim-up and density gradient centrifugation washing techniques prior to freezing.

Centrifugal force: a few surprises

International Nuclear Information System (INIS)

Abramowicz, M.A.; Max-Planck-Institut fuer Physik und Astrophysik, Garching

1990-01-01

The need for a rather fundamental revision in understanding of the nature of the centrifugal force is discussed. It is shown that in general relativity (and contrary to the situation in Newtonian theory) rotation of a reference frame is a necessary but not sufficient condition for the centrifugal force to appear. A sufficient condition for its appearance, in the instantaneously corotating reference frame of a particle, is that the particle motion in space (observed in the global rest frame) differs from a photon trajectory. The direction of the force is the same as that of the gradient of the effective potential for photon motion. In some cases, the centrifugal force will attract towards the axis of rotation. (author)

Plasma instability of a vacuum arc centrifuge

International Nuclear Information System (INIS)

Hole, M.J.; Dallaqua, R.S.; Simpson, S.W.; Del Bosco, E.

2002-01-01

Ever since conception of the vacuum arc centrifuge in 1980, periodic fluctuations in the ion saturation current and floating potential have been observed in Langmuir probe measurements in the rotation region of a vacuum arc centrifuge. In this work we develop a linearized theoretical model to describe a range of instabilities in the vacuum arc centrifuge plasma column, and then test the validity of the description through comparison with experiment. We conclude that the observed instability is a 'universal' instability, driven by the density gradient, in a plasma with finite conductivity

Simulation of ultra-long term behavior in HLW near-field by centrifugal model test. Part 1. Development of centrifugal equipment and centrifuge model test method

International Nuclear Information System (INIS)

Nishimoto, Soshi; Okada, Tetsuji; Sawada, Masataka

2011-01-01

The objective of this paper is to develop a centrifugal equipment which can continuously be run for a long time and a model test method in order to evaluate a long term behavior which is a coupled thermo-hydro-mechanical processes in the high level wastes geological disposal repository and the neighborhood (called 'near-field'). The centrifugal equipment of CRIEPI, 'CENTURY5000-THM', developed in the present study is able to run continuously up to six months. Therefore, a long term behavior in the near-field can be simulated in a short term, for instance, the behavior for 5000 equivalent years can be simulated in six months by centrifugalizing 100 G using a 1/100 size model. We carried out a test using a nylon specimen in a centrifugal force field of 30 G and confirmed the operations of CENTURY5000-THM, control and measurement for 11 days. As the results, it was able to control the stress in the pressure vessel and measure the values of strain, temperature and pressure. And, as a result of scanning the small model of near-field including the metal overpack, bentonite buffer and rock by a medical X-rays CT scanner, the internal structure of the model was able to be evaluated when the metal artifact was reduced. From these results, the evaluation for a long term behavior of a disposal repository by the method of centrifugal model test became possible. (author)

Comparison of the lysis centrifugation method with the conventional blood culture method in cases of sepsis in a tertiary care hospital.

Science.gov (United States)

Parikh, Harshal R; De, Anuradha S; Baveja, Sujata M

2012-07-01

Physicians and microbiologists have long recognized that the presence of living microorganisms in the blood of a patient carries with it considerable morbidity and mortality. Hence, blood cultures have become critically important and frequently performed test in clinical microbiology laboratories for diagnosis of sepsis. To compare the conventional blood culture method with the lysis centrifugation method in cases of sepsis. Two hundred nonduplicate blood cultures from cases of sepsis were analyzed using two blood culture methods concurrently for recovery of bacteria from patients diagnosed clinically with sepsis - the conventional blood culture method using trypticase soy broth and the lysis centrifugation method using saponin by centrifuging at 3000 g for 30 minutes. Overall bacteria recovered from 200 blood cultures were 17.5%. The conventional blood culture method had a higher yield of organisms, especially Gram positive cocci. The lysis centrifugation method was comparable with the former method with respect to Gram negative bacilli. The sensitivity of lysis centrifugation method in comparison to conventional blood culture method was 49.75% in this study, specificity was 98.21% and diagnostic accuracy was 89.5%. In almost every instance, the time required for detection of the growth was earlier by lysis centrifugation method, which was statistically significant. Contamination by lysis centrifugation was minimal, while that by conventional method was high. Time to growth by the lysis centrifugation method was highly significant (P value 0.000) as compared to time to growth by the conventional blood culture method. For the diagnosis of sepsis, combination of the lysis centrifugation method and the conventional blood culture method with trypticase soy broth or biphasic media is advocable, in order to achieve faster recovery and a better yield of microorganisms.

Centrifuge in space fluid flow visualization experiment

Science.gov (United States)

Arnold, William A.; Wilcox, William R.; Regel, Liya L.; Dunbar, Bonnie J.

1993-01-01

A prototype flow visualization system is constructed to examine buoyancy driven flows during centrifugation in space. An axial density gradient is formed by imposing a thermal gradient between the two ends of the test cell. Numerical computations for this geometry showed that the Prandtl number plays a limited part in determining the flow.

Discussion of various flow calculation methods in high-speed centrifuges

International Nuclear Information System (INIS)

Louvet, P.; Cortet, C.

1979-01-01

The flow in high-speed centrifuges for the separation of uranium isotopes has been studied in the frame of linearized theory for long years. Three different methods have been derived for viscous compressible flow with small Ekman numbers and high Mach numbers: - numerical solution of flow equation by finite element method and Gaussian elimination (Centaure Code), - boundary layer theory using matched asymptotic expansions, - the so called eigenfunction method slightly modified. The mathematical assumptions, the easiness and the accuracy of the computations are compared. Numerical applications are performed successively for thermal countercurrent centrifuges with or without injections

Preparation of targets by a centrifugal method

International Nuclear Information System (INIS)

Richaud, J.P.

1979-01-01

For β and γ in-beam spectroscopy measurements, a centrifugal technique has been developed to produce targets of powdered isotopic materials. Plastic or metallic backings and suitable organic solutions are described. With this method, targets in the range 1-50 mg/cm 2 have been obtained. (orig.)

Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma.

Science.gov (United States)

Shin, Hyeok-Soo; Woo, Heung-Myong; Kang, Byung-Jae

2017-06-26

Platelet-rich plasma (PRP) has been expected for regenerative medicine because of its growth factors. However, there is considerable variability in the recovery and yield of platelets and the concentration of growth factors in PRP preparations. The aim of this study was to identify optimal relative centrifugal force and spin time for the preparation of PRP from canine blood using a double-centrifugation tube method. Whole blood samples were collected in citrate blood collection tubes from 12 healthy beagles. For the first centrifugation step, 10 different run conditions were compared to determine which condition produced optimal recovery of platelets. Once the optimal condition was identified, platelet-containing plasma prepared using that condition was subjected to a second centrifugation to pellet platelets. For the second centrifugation, 12 different run conditions were compared to identify the centrifugal force and spin time to produce maximal pellet recovery and concentration increase. Growth factor levels were estimated by using ELISA to measure platelet-derived growth factor-BB (PDGF-BB) concentrations in optimised CaCl 2 -activated platelet fractions. The highest platelet recovery rate and yield were obtained by first centrifuging whole blood at 1000 g for 5 min and then centrifuging the recovered platelet-enriched plasma at 1500 g for 15 min. This protocol recovered 80% of platelets from whole blood and increased platelet concentration six-fold and produced the highest concentration of PDGF-BB in activated fractions. We have described an optimised double-centrifugation tube method for the preparation of PRP from canine blood. This optimised method does not require particularly expensive equipment or high technical ability and can readily be carried out in a veterinary clinical setting.

Gas centrifuge purge method

Science.gov (United States)

Theurich, Gordon R.

1976-01-01

1. In a method of separating isotopes in a high speed gas centrifuge wherein a vertically oriented cylindrical rotor bowl is adapted to rotate about its axis within an evacuated chamber, and wherein an annular molecular pump having an intake end and a discharge end encircles the uppermost portion of said rotor bowl, said molecular pump being attached along its periphery in a leak-tight manner to said evacuated chamber, and wherein end cap closure means are affixed to the upper end of said rotor bowl, and a process gas withdrawal and insertion system enters said bowl through said end cap closure means, said evacuated chamber, molecular pump and end cap defining an upper zone at the discharge end of said molecular pump, said evacuated chamber, molecular pump and rotor bowl defining a lower annular zone at the intake end of said molecular pump, a method for removing gases from said upper and lower zones during centrifuge operation with a minimum loss of process gas from said rotor bowl, comprising, in combination: continuously measuring the pressure in said upper zone, pumping gas from said lower zone from the time the pressure in said upper zone equals a first preselected value until the pressure in said upper zone is equal to a second preselected value, said first preselected value being greater than said second preselected value, and continuously pumping gas from said upper zone from the time the pressure in said upper zone equals a third preselected value until the pressure in said upper zone is equal to a fourth preselected value, said third preselected value being greater than said first, second and fourth preselected values.

Density-Gradient Mediated Band Extraction of Leukocytes from Whole Blood Using Centrifugo-Pneumatic Siphon Valving on Centrifugal Microfluidic Discs

Science.gov (United States)

Kearney, Sinéad M.; Kilcawley, Niamh A.; Early, Philip L.; Glynn, Macdara T.; Ducrée, Jens

2016-01-01

Here we present retrieval of Peripheral Blood Mononuclear Cells by density-gradient medium based centrifugation for subsequent analysis of the leukocytes on an integrated microfluidic “Lab-on-a-Disc” cartridge. Isolation of white blood cells constitutes a critical sample preparation step for many bioassays. Centrifugo-pneumatic siphon valves are particularly suited for blood processing as they function without need of surface treatment and are ‘low-pass’, i.e., holding at high centrifugation speeds and opening upon reduction of the spin rate. Both ‘hydrostatically’ and ‘hydrodynamically’ triggered centrifugo-pneumatic siphon valving schemes are presented. Firstly, the geometry of the pneumatic chamber of hydrostatically primed centrifugo-pneumatic siphon valves is optimised to enable smooth and uniform layering of blood on top of the density-gradient medium; this feature proves to be key for efficient Peripheral Blood Mononuclear Cell extraction. A theoretical analysis of hydrostatically primed valves is also presented which determines the optimum priming pressure for the individual valves. Next, ‘dual siphon’ configurations for both hydrostatically and hydrodynamically primed centrifugo-pneumatic siphon valves are introduced; here plasma and Peripheral Blood Mononuclear Cells are extracted through a distinct siphon valve. This work represents a first step towards enabling on disc multi-parameter analysis. Finally, the efficiency of Peripheral Blood Mononuclear Cells extraction in these structures is characterised using a simplified design. A microfluidic mechanism, which we termed phase switching, is identified which affects the efficiency of Peripheral Blood Mononuclear Cell extraction. PMID:27167376

An Experiment Using Sucrose Density Gradients in the Undergraduate Biochemistry Laboratory.

Science.gov (United States)

Turchi, Sandra L.; Weiss, Monica

1988-01-01

Describes an experiment to be performed in an undergraduate biochemistry laboratory that is based on a gradient centrifugation system employing a simple bench top centrifuge, a freezer, and frozen surcose gradient solution to separate macromolecules and subcellular components. (CW)

Physical simulations using centrifuge techniques

International Nuclear Information System (INIS)

Sutherland, H.J.

1981-01-01

Centrifuge techniques offer a technique for doing physical simulations of the long-term mechanical response of deep ocean sediment to the emplacement of waste canisters and to the temperature gradients generated by them. Preliminary investigations of the scaling laws for pertinent phenomena indicate that the time scaling will be consistent among them and equal to the scaling factor squared. This result implies that this technique will permit accelerated-life-testing of proposed configurations; i.e, long-term studies may be done in relatively short times. Presently, existing centrifuges are being modified to permit scale model testing. This testing will start next year

Centrifugation

International Nuclear Information System (INIS)

Subbaramajer.

1983-01-01

The theoretical analysis of the processes taking place at centrifugal method of isotope separation taking into account the latest investigations, in particular, investigation of velocity field applying the theory of boundary layers in rotating gas is conducted. As a result of using power computers for the solution of hydrodynamics equations by numerical methods sufficiently exact solutions of main hydrodynamic equations, reflecting the real centrifuge construction are derived. The increase of calculation accuracy of the flow field reflected also on the accuracy of the diffusion equation solution. Three parameters of similarity (height of transfer unit, flow, mass transfer coefficient) and their connection with the flow field, elementary separation coefficient in a cetrifugal field and molecular diffusion coefficient is determined. Modified formulas for the separation coefficient and separation centrifuge power taking into account similarity parameter changes over the axis are derived. The possibility of determining the system of controlled parameters optimizing the separation centrifuge power is shown

Detection methods for centrifugal microfluidic platforms

DEFF Research Database (Denmark)

Burger, Robert; Amato, Letizia; Boisen, Anja

2016-01-01

Centrifugal microfluidics has attracted much interest from academia as well as industry, since it potentially offers solutions for affordable, user-friendly and portable biosensing. A wide range of so-called fluidic unit operations, e.g. mixing, metering, liquid routing, and particle separation...... for the centrifugal microfluidics platform and cover optical as well as mechanical and electrical detection principles....

Instabilities expected to exist in a gas centrifuge

International Nuclear Information System (INIS)

Sakurai, Takeo

1977-01-01

A typical counter current type centrifuge of long bowl geometry is schematically shown. At first glance, the main flow field in this centrifuge can be taken as a swirling pipe flow. Taking in mind the operating gas (uranium hexafluoride) the temperature of which is 20 deg C and the peripheral pressure 10 torrs, the density and pressure obey the barometric relation in which the gravity is replaced by the centrifugal acceleration; in a thermally driven centrifuge, an additional weak temperature gradient appears along the axial direction. These situations are similar to those in the earth's atmosphere. So, it is stressed that the interior of a gas centrifuge is a new kind of rotating atmosphere and offers a 'new face' in the field of geophysical fluid dynamics. Instabilities in inviscid case and the destabilizing effects of the diffusivity are thus discussed together with the effects of the mechanical vibrations of the centrifuge, and vortex breakdown phenomena

Numerical study of the effects of curvature on the fluid dynamics of gas centrifuges

International Nuclear Information System (INIS)

Jordan, J.A.; Gunzburger, M.D.; Wood, H.G. III.

1983-06-01

A finite element method for the approximate solution of the flow in rapidly rotating gas centrifuges is presented. The Onsager model, as amended by Maslen, is used in deriving the model equations to be discretized. The pancake effects are not assumed in the model, i.e., curvature terms are retained. To show the effects of these terms on the hydrodynamics of a gas centrifuge, numerical examples done with and without these curvature terms are presented and compared. Two flow models are used for the examples, one for flow driven by a linear temperature gradient along the wall and the other for flow driven by axial mass fluxes through the end caps of the centrifuge

Description of a double centrifugation tube method for concentrating canine platelets.

Science.gov (United States)

Perazzi, Anna; Busetto, Roberto; Martinello, Tiziana; Drigo, Michele; Pasotto, Daniela; Cian, Francesco; Patruno, Marco; Iacopetti, Ilaria

2013-07-22

To evaluate the efficiency of platelet-rich plasma preparations by means of a double centrifugation tube method to obtain platelet-rich canine plasma at a concentration at least 4 times higher than the baseline value and a concentration of white blood cells not exceeding twice the reference range. A complete blood count was carried out for each sample and each concentrate. Whole blood samples were collected from 12 clinically healthy dogs (consenting blood donors). Blood was processed by a double centrifugation tube method to obtain platelet concentrates, which were then analyzed by a flow cytometry haematology system for haemogram. Platelet concentration and white blood cell count were determined in all samples. Platelet concentration at least 4 times higher than the baseline value and a white blood cell count not exceeding twice the reference range were obtained respectively in 10 cases out of 12 (83.3%) and 11 cases out of 12 (91.6%). This double centrifugation tube method is a relatively simple and inexpensive method for obtaining platelet-rich canine plasma, potentially available for therapeutic use to improve the healing process.

Present status of centrifuge method for uranium enrichment and PNC plant

International Nuclear Information System (INIS)

Nishikido, Yoshikazu

1977-01-01

Recentry, the tendency to adopt atomic energy owing to the oil shock, the delay in the construction of nuclear power stations worldwide, the uncertainty in the utilization of plutonium, and the prospect of supplying natural uranium are the situations affecting uranium enrichment. Anyway, the enrichment capacity in the world must be increased by the early years of 1980 s. The uranium enrichment technology by centrifugal method is being developed in various countries under strict control of informations, therefore the details are not known, but the general state in Great Britain, F.R. of Germany, Netherlands, U.S.A. and Japan is explained. The development of the centrifugal enrichment method in Japan was designated in 1972 as the national project aiming at operating the enriching plant with international competitive power by 1985. The PNC undertook the development work, and the research and development include the development of a centrifuge, cascade test, life span test, the development of the mass production technology, and safety test. The especially notable matter in this period was the rapid progress of a supercritical type centrifuge. It is judged that the technical basis for constructing a pilot plant has been established. The site for the pilot plant is being prepared now in the Ningyo Pass Mine, PNC, and the enrichment plant with 7000 centrifuges will be constructed there. The outline of the plant and the schedule for the construction are described. (Kako, I.)

Modified method for labeling human platelets with indium-111 oxine using albumin density-gradient separation

International Nuclear Information System (INIS)

Bunting, R.W.; Callahan, R.J.; Finkelstein, S.; Lees, R.S.; Strauss, H.W.

1982-01-01

When labeling platelets with indium-111 oxine, albumin density-gradient separation minimizes the time spent to resuspend those platelets that have been centrifuged against a hard surface. Labeling efficiency or platelet viability, as measured by platelet survival or aggregation with adenosine diphosphate, are not adversely affected

Devices and methods of operation thereof for providing stable flow for centrifugal compressors

Science.gov (United States)

Skoch, Gary J. (Inventor); Stevens, Mark A. (Inventor); Jett, Thomas A. (Inventor)

2008-01-01

Centrifugal compressor flow stabilizing devices and methods of operation thereof are disclosed that act upon the flow field discharging from the impeller of a centrifugal compressor and modify the flow field ahead of the diffuser vanes such that flow conditions contributing to rotating stall and surge are reduced or even eliminated. In some embodiments, shaped rods and methods of operation thereof are disclosed, whereas in other embodiments reverse-tangent air injection devices and methods are disclosed.

Centrifuge-operated specimen staining method and apparatus

Science.gov (United States)

Clarke, Mark S. F. (Inventor); Feeback, Daniel L. (Inventor)

1999-01-01

A method of staining preselected, mounted specimens of either biological or nonbiological material enclosed within a staining chamber where the liquid staining reagents are applied and removed from the staining chamber using hypergravity as the propelling force. In the preferred embodiment, a spacecraft-operated centrifuge and method of diagnosing biological specimens while in orbit, characterized by hermetically sealing a shell assembly. The assembly contains slide stain apparatus with computer control therefor, the operative effect of which is to overcome microgravity, for example on board an International Space Station.

Runtime and Inversion Impacts on Estimation of Moisture Retention Relations by Centrifuge

Science.gov (United States)

Sigda, J. M.; Wilson, J. L.

2003-12-01

Standard laboratory methods in soil physics for measuring the moisture retention relation (drainage matric potential-volumetric moisture content relation) are each limited to only part of the moisture content range. Centrifuge systems allow intensive accurate measurements across much of the saturation range, and typically require much less time than traditional laboratory methods. An initially liquid-saturated sample is subjected to a stepwise-increasing series of angular velocities while carefully monitoring changes in liquid content. Angular velocity is held constant until the capillary and centrifugal forces equilibrate, forcing liquid flux to zero, and then a final average liquid content is noted. The procedure is repeated after increasing the angular velocity. Centrifuge measurement time is greatly reduced because the centrifugal body force gradient can far exceed the driving forces utilized in standard lab methods. Widely-used in the petroleum industry for decades, centrifuge measurement of moisture retention relations is seldom encountered in the soil physics or vadose hydrology literatures. Yet there is a need to better understand and improve the experimental methodology given the increasing number of centrifuges employed in these fields. Errors in centrifuge measurement of moisture retention relations originate from both experimental protocol and from data inversion. Like standard methods, centrifuge methods assume equilibrium conditions, and so are sensitive to errors introduced by insufficient runtimes. Unlike standard methods, centrifuge experiments require inversion of the angular velocity and average sample moisture content data to a location-specific pair of matric potential and moisture content values, The force balance causes matric potential and moisture content to vary with sample length while the sample is spinning. Numerous data inversion techniques exist, each yielding different moisture retention relations. We present analyses demonstrating

Evaluation of single and double centrifugation tube methods for concentrating equine platelets.

Science.gov (United States)

Argüelles, D; Carmona, J U; Pastor, J; Iborra, A; Viñals, L; Martínez, P; Bach, E; Prades, M

2006-10-01

The aim of this study was to evaluate single and double centrifugation tube methods for concentrating equine platelets. Whole blood samples were collected from clinically normal horses and processed by use of single and double centrifugation tube methods to obtain four platelet concentrates (PCs): PC-A, PC-B, PC-C, and PC-D, which were analyzed using a flow cytometry hematology system for hemogram and additional platelet parameters (mean platelet volume, platelet distribution width, mean platelet component concentration, mean platelet component distribution width). Concentrations of transforming growth factor beta 1 (TGF-beta(1)) were determined in all the samples. Platelet concentrations for PC-A, PC-B, PC-C, and PC-D were 45%, 44%, 71%, and 21% higher, respectively, compared to the same values for citrated whole blood samples. TGF-beta(1) concentrations for PC-A, PC-B, PC-C, and PC-D were 38%, 44%, 44%, and 37% higher, respectively, compared to citrated whole blood sample values. In conclusion, the single and double centrifugation tube methods are reliable methods for concentrating equine platelets and for obtaining potentially therapeutic TGF-beta(1) levels.

Block-conjugate-gradient method

International Nuclear Information System (INIS)

McCarthy, J.F.

1989-01-01

It is shown that by using the block-conjugate-gradient method several, say s, columns of the inverse Kogut-Susskind fermion matrix can be found simultaneously, in less time than it would take to run the standard conjugate-gradient algorithm s times. The method improves in efficiency relative to the standard conjugate-gradient algorithm as the fermion mass is decreased and as the value of the coupling is pushed to its limit before the finite-size effects become important. Thus it is potentially useful for measuring propagators in large lattice-gauge-theory calculations of the particle spectrum

An Optimal Design Method of Centrifugal Compressors in Consideration of the Efficiency and the Noise

International Nuclear Information System (INIS)

Ha, K. G.; Sung, S. M.; Kang, S. H.

2007-01-01

A centrifugal compressor is a principal part of the fuelcell vehicles, aircraft and home appliances. Therefore not only efficiency but also compact size and a low operation RPM for noise reducing turn into important criteria of centrifugal compressors design. But those criteria are in conflict each other often. In the case of a RPM in particular, it is profitable to lower the RPM for a noise reduction and an endurance. But for a compact size and a light weight, the reverse has a beneficial effect undoubtedly. So it is necessary to introduce a new optimization concept in the centrifugal compressor design. An one dimensional optimal design method for the centrifugal compressor considering a impeller, a vaneless diffuser and a volute at a time is described. The new optimization process and underlying design methods of centrifugal compressors and some optimal design results are included in the paper

Dai-Kou type conjugate gradient methods with a line search only using gradient.

Science.gov (United States)

Huang, Yuanyuan; Liu, Changhe

2017-01-01

In this paper, the Dai-Kou type conjugate gradient methods are developed to solve the optimality condition of an unconstrained optimization, they only utilize gradient information and have broader application scope. Under suitable conditions, the developed methods are globally convergent. Numerical tests and comparisons with the PRP+ conjugate gradient method only using gradient show that the methods are efficient.

The study of multicomponent separation of Xe isotope by centrifugal method

International Nuclear Information System (INIS)

Jinyan Wu; Fu Zhuge

1996-01-01

The element Xe has nine isotopes in nature, the separation performance of each component mutually affects the others, so the binary separation theory can't be employed to study the multicomponent separation. Especially, when the molecular wight of a certain component is in the middle of its isotope components, the effect of the others on this component must be considered. In this paper, first, the multicomponent separation of Xe isotopes in a gas centrifuge is studied, with the consideration of the effect of the concentration on the diffusion coefficient and average molecular weight. The multicomponent diffusion equations are solved by the finite difference method. Second, the enrichment of Xe isotopes in a cascade is studied. On the basis of the study of a gas centrifuge, the simplified separation equations of a gas centrifuge for cascade calculation are obtained. Furthermore, the complete equations of the cascade separation are established according to the conservation of mass of each component and solved by a numerical method. The study of this paper can be extended for other isotope separation calculations. (author)

[A thermodynamic study on bovine spermatozoa by microcalorimetry after Percoll density-gradient centrifugation - experimental probe of its utility in andrology].

Science.gov (United States)

Fischer, C; Scherfer-Brähler, V; Müller-Schlösser, F; Schröder-Printzen, I; Weidner, W

2007-05-01

Microcalorimetric measurements can be used for recording exothermic or endothermic summation effects of a great variety of biological processes. The aim of the present study was to examine the usefullness of the microcalorimetry method to characterise the biological activity of spermatozoa. The heat flow of bovine fresh sperm as well as cryosperm samples were measured after Percoll density-gradient centrifugation in a 4-channel microcalorimeter. Various calibration times, volumes of samples and sperm concentrations were tested and analysed. Sperm concentration was recorded by a computer-assisted, computer-aided software system method (CASA). Using a calibration time of 15 minutes, the heat signal of the fresh and cryosperm samples showed a characteristic peak after 39.5 min and 38.1 min (mean), respectively, with a significant correlation to sample volume and sperm concentration (p < 0.05). For obtaining the best results, a sample volume of 1 ml and a sperm concentration of more than 50 x 10 (6)/mL was used. With microcalorimetric measurements the biological activity of spermatozoa could be recorded for reproducible results, thus opening the way to an automatised ejaculate analysis in the future. More investigations are necessary to correlate microcalorimetric parameters with semen function.

Investigation of CFD calculation method of a centrifugal pump with unshrouded impeller

Science.gov (United States)

Wu, Dazhuan; Yang, Shuai; Xu, Binjie; Liu, Qiaoling; Wu, Peng; Wang, Leqin

2014-03-01

Currently, relatively large errors are found in numerical results in some low-specific-speed centrifugal pumps with unshrouded impeller because the effect of clearances and holes are not accurately modeled. Establishing an accurate analytical model to improve performance prediction accuracy is therefore necessary. In this paper, a three-dimensional numerical simulation is conducted to predict the performance of a low-specific-speed centrifugal pump, and the modeling, numerical scheme, and turbulent selection methods are discussed. The pump performance is tested in a model pump test bench, and flow rate, head, power and efficiency of the pump are obtained. The effect of taking into consideration the back-out vane passage, clearance, and balance holes is analyzed by comparing it with experimental results, and the performance prediction methods are validated by experiments. The analysis results show that the pump performance can be accurately predicted by the improved method. Ignoring the back-out vane passage in the calculation model of unshrouded impeller is found to generate better numerical results. Further, the calculation model with the clearances and balance holes can obviously enhance the numerical accuracy. The application of disconnect interface can reduce meshing difficulty but increase the calculation error at the off-design operating point at the same time. Compared with the standard k-ɛ, renormalization group k-ɛ, and Spalart-Allmars models, the Realizable k-ɛ model demonstrates the fastest convergent speed and the highest precision for the unshrouded impeller flow simulation. The proposed modeling and numerical simulation methods can improve the performance prediction accuracy of the low-specific-speed centrifugal pumps, and the modeling method is especially suitable for the centrifugal pump with unshrouded impeller.

The effects of curvature on the flow field in rapidly rotating gas centrifuges

International Nuclear Information System (INIS)

Wood, H.G.; Jordan, J.A.

1984-01-01

The effects of curvature on the fluid dynamics of rapidly rotating gas centrifuges are studied. A governing system of a linear partial differential equation and boundary conditions is derived based on a linearization of the equations for viscous compressible flow. This system reduces to the Onsager pancake model if the effects of curvature are neglected. Approximations to the solutions of the governing equations with and without curvature terms are obtained via a finite-element method. Two examples are considered: first where the flow is driven by a thermal gradient at the wall of the centrifuge, and then for the flow being driven by the introduction and removal of mass through the ends of the centrifuge. Comparisons of the results obtained show that, especially for the second example, the inclusion of the terms due to curvature in the model can have an appreciable effect on the solution. (author)

Evaluating the efficacy of a centrifugation-flotation method for extracting Ascaris ova from soil

DEFF Research Database (Denmark)

Cranston, Imogen; Teoh, Penelope J.; baker, Sarah M.

2016-01-01

method to extract STH ova from soil makes it challenging to examine whether the use of latrines may or may not have an effect on environmental contamination with ova. The present study evaluated the recovery rate of a method developed to extract STH ova from soil. Methods: The adapted centrifugation...... with increasing soil moisture content, particle size and organic matter content. The association between recovery rate and organic matter content was statistically significant. Conclusions: The present study identified a low recovery rate for an adapted centrifugation-flotation method, although this was similar...

STOCHASTIC GRADIENT METHODS FOR UNCONSTRAINED OPTIMIZATION

Directory of Open Access Journals (Sweden)

Nataša Krejić

2014-12-01

Full Text Available This papers presents an overview of gradient based methods for minimization of noisy functions. It is assumed that the objective functions is either given with error terms of stochastic nature or given as the mathematical expectation. Such problems arise in the context of simulation based optimization. The focus of this presentation is on the gradient based Stochastic Approximation and Sample Average Approximation methods. The concept of stochastic gradient approximation of the true gradient can be successfully extended to deterministic problems. Methods of this kind are presented for the data fitting and machine learning problems.

Centrifugation. A theoretical study of oxygen enrichment by centrifugation

Energy Technology Data Exchange (ETDEWEB)

Kierkegaard, P.; Raetz, E.

1998-12-01

In the present paper we first investigate what happens if we fill a cylinder with air, close it and rotate it. The results show that no matter which peripheral speed is used, it is not possible by means of the radial separation effect alone, to enrich the oxygen concentration from the previous 21% to more then 23.3%, which is of no practical value. In case of a too low enrichment in one centrifuge, the wanted material from this centrifuge can be used as an input for a second centrifuge and so on, in this way forming a cascade of centrifuges. Oxygen will be enriched in each step, until the desired concentration is reached. Cascading was the technology in the very beginning by enrichment plants for uraniumhexaflouride, used for atomic weapons and nuclear power plants. In this study we try to avoid cascading by aiming for higher separation factors. Therefore, we next investigate the possibilities of using a countercurrent centrifuge where in principle the enriched gas is subjected to several centrifugation in the same centrifuge. The calculations show, that in this way it is possible to produce nearly a 100% pure oxygen (polluted with some heavier molecules like argon) in one machine. Our third step was to calculate the amount of oxygen produced per hour. Using a countercurrent centrifuge of the Zippe type, 100 cm high and 20 cm in diameter, it is or will be possible in the near future to produce 17 g enriched air per hour enriched to 50% oxygen. That corresponds to processing 1 m{sup 3} atmospherical air in the period of approximately 24 hours. This is not very impressive. Our fourth step was to estimate the amount of power used for producing this amount of oxygen. A rough, but complicated, estimate shows that the power consumption at the production level will be about the double of the consumption used today. The overall conclusion is, that centrifugation as a production method for oxygen (or nitrogen) will not be competitive with the currently used method in the

Desvio da proporção de sexo e da integridade do DNA dos espermatozóides bovinos centrifugados em gradientes de densidade contínuos Alteration of sex ratio and DNA integrity of bovine sperm centrifuged in continuous density gradients

Directory of Open Access Journals (Sweden)

Alberto Lopes Gusmão

2010-03-01

Full Text Available O objetivo, neste trabalho, foi verificar o desvio da proporção de sexo e a presença de fragmentação do DNA, pela técnica de TUNEL (“In situ terminal deoxinucleotidyl transferase mediated dUTP nick end labeling assay”, em espermatozoides bovinos centrifugados em gradientes de densidade de Percoll ou OptiPrep durante a separação espermática. Doses de sêmen de touros foram descongeladas, e cerca de 40 milhões de espermatozoides foram depositados sobre cada gradiente de densidade compostos por Percoll ou OptiPrep com três camadas entre 1.110g/mL e 1.123g/mL, em tubos de 15mL, em que permaneceram por 24h a 4°C antes da deposição dos espermatozoides. Os tubos foram centrifugados a 500xg por 15min a 22°C. Os sobrenadantes foram aspirados, e os sedimentos, recuperados para verificação da fragmentação do DNA pela técnica de TUNEL. Obteve-se um desvio dos embriões produzidos in vitro para fêmeas no gradiente de Percoll (62% de fêmeas, em relação aos grupos OptiPrep e Controle (47,1 e 48,7% de fêmeas, respectivamente. Não foi detectada fragmentação do DNA dos espermatozoides nas amostras centrifugadas, tanto no gradiente de Percoll quanto de OptiPrep. Dessa forma, foi possível realizar a sexagem espermática, com uma maior porcentagem de espermatozoides X do que o grupo controle, por meio de metodologia mais simples e sem provocar danos ao DNA dos espermatozoides.The objective of the present study was to verify the sex ratio and presence of DNA fragmentation by TUNEL technique (In situ terminal deoxinucleotidyl transferase mediated dUTP nick end labeling assay in bovine spermatozoa centrifuged in density gradients of Percoll or OptiPrep during the sperm separation. Approximately 40 million of frozen/thawed bovine spermatozoa were deposited on each density gradient composed of Percoll or OptiPrep with three layers ranging from 1.110g/mL to 1.123g/mL in polystyrene tubes of 15mL. The tubes were kept at 4°C for 24h before

Centrifuge apparatus

Science.gov (United States)

Sartory, Walter K.; Eveleigh, John W.

1976-01-01

A method and apparatus for operating a continuous flow blood separation centrifuge are provided. The hematocrit of the entrant whole blood is continuously maintained at an optimum constant value by the addition of plasma to the entrant blood. The hematocrit of the separated red cells is monitored to indicate the degree of separation taking place, thereby providing a basis for regulating the flow through the centrifuge.

Large Airborne Full Tensor Gradient Data Inversion Based on a Non-Monotone Gradient Method

Science.gov (United States)

Sun, Yong; Meng, Zhaohai; Li, Fengting

2018-03-01

Following the development of gravity gradiometer instrument technology, the full tensor gravity (FTG) data can be acquired on airborne and marine platforms. Large-scale geophysical data can be obtained using these methods, making such data sets a number of the "big data" category. Therefore, a fast and effective inversion method is developed to solve the large-scale FTG data inversion problem. Many algorithms are available to accelerate the FTG data inversion, such as conjugate gradient method. However, the conventional conjugate gradient method takes a long time to complete data processing. Thus, a fast and effective iterative algorithm is necessary to improve the utilization of FTG data. Generally, inversion processing is formulated by incorporating regularizing constraints, followed by the introduction of a non-monotone gradient-descent method to accelerate the convergence rate of FTG data inversion. Compared with the conventional gradient method, the steepest descent gradient algorithm, and the conjugate gradient algorithm, there are clear advantages of the non-monotone iterative gradient-descent algorithm. Simulated and field FTG data were applied to show the application value of this new fast inversion method.

SEAL FOR HIGH SPEED CENTRIFUGE

Science.gov (United States)

Skarstrom, C.W.

1957-12-17

A seal is described for a high speed centrifuge wherein the centrifugal force of rotation acts on the gasket to form a tight seal. The cylindrical rotating bowl of the centrifuge contains a closure member resting on a shoulder in the bowl wall having a lower surface containing bands of gasket material, parallel and adjacent to the cylinder wall. As the centrifuge speed increases, centrifugal force acts on the bands of gasket material forcing them in to a sealing contact against the cylinder wall. This arrangememt forms a simple and effective seal for high speed centrifuges, replacing more costly methods such as welding a closure in place.

Microfluidic size separation of cells and particles using a swinging bucket centrifuge.

Science.gov (United States)

Yeo, Joo Chuan; Wang, Zhiping; Lim, Chwee Teck

2015-09-01

Biomolecular separation is crucial for downstream analysis. Separation technique mainly relies on centrifugal sedimentation. However, minuscule sample volume separation and extraction is difficult with conventional centrifuge. Furthermore, conventional centrifuge requires density gradient centrifugation which is laborious and time-consuming. To overcome this challenge, we present a novel size-selective bioparticles separation microfluidic chip on a swinging bucket minifuge. Size separation is achieved using passive pressure driven centrifugal fluid flows coupled with centrifugal force acting on the particles within the microfluidic chip. By adopting centrifugal microfluidics on a swinging bucket rotor, we achieved over 95% efficiency in separating mixed 20 μm and 2 μm colloidal dispersions from its liquid medium. Furthermore, by manipulating the hydrodynamic resistance, we performed size separation of mixed microbeads, achieving size efficiency of up to 90%. To further validate our device utility, we loaded spiked whole blood with MCF-7 cells into our microfluidic device and subjected it to centrifugal force for a mere duration of 10 s, thereby achieving a separation efficiency of over 75%. Overall, our centrifugal microfluidic device enables extremely rapid and label-free enrichment of different sized cells and particles with high efficiency.

Multiple-isotope separation in gas centrifuge

International Nuclear Information System (INIS)

Wood, Houston G.; Mason, T.C.; Soubbaramayer

1996-01-01

In previous works, the Onsager's pancake equation was used to provide solution to the internal counter-current flow field, which is necessary to calculate solutions to the isotope transport equation. The diffusion coefficient was assumed to be constant which is a good approximation for gases with large molecular weights, and small differences in the molecular weights of the various isotopes. A new optimization strategy was presented for determining the operating parameters of a gas centrifuge to be used for multiple-component isotope separation. Scoop drag, linear wall temperature gradient, the feed rate ant the cut have been chosen as operating parameters for the optimization. The investigation was restricted to a single centrifuge, and the problem of cascading for multiple-isotope separation was not addressed. The model describing the flow and separation phenomena in centrifuge includes a set of equations describing the fluid dynamics of the counter-current flow and the diffusion equations written for each isotope of the mixture. In this paper, an optimization algorithm is described and applied to an example for the re enrichment of spent reactor uranium

Effect of centrifugation and washing on adipose graft viability: a new method to improve graft efficiency.

Science.gov (United States)

Hoareau, Laurence; Bencharif, Karima; Girard, Anne-Claire; Gence, Lydie; Delarue, Pierre; Hulard, Olivier; Festy, Franck; Roche, Regis

2013-05-01

Adipose tissue grafting is a promising method in the field of surgical filling. We studied the effect of centrifugation on fat grafts, and we propose an optimised protocol for the improvement of adipose tissue viability. Adipose tissue was subjected to different centrifugations, and the volumes of interstitial liquid and oil released were measured to choose the optimal condition. Tissue from this condition was then compared to tissue obtained from two traditional techniques: strong centrifugation (commonly 3 min at 3000 rpm/900 g), and decantation, by injecting into immunodeficient mice. The cytokine interleukin-6 (IL-6) and chemokine monocyte chemotactic protein-1 (MCP-1) were assayed 24 h post-injection, and after 1 month of grafting the state of the lipografts was evaluated through macroscopic and histological analysis, with oil gap area measurement. Strong centrifugation (900 g, 1800 g) is deleterious for adipose tissue because it leads to until threefold more adipocyte death compared to low centrifugation (100 g, 400 g). In addition, mice injected with strong centrifuged and non-centrifuged adipose tissue have higher rates of blood IL-6 and MCP-1, compared to those grafted with soft centrifuged fat. Moreover, extensive lipid vacuoles were detectable on histological sections of the non-centrifuged lipografts, whereas lipografts from soft centrifugation contain a higher amount of connective tissue containing collagen fibres. It is necessary to wash and centrifuge adipose tissue before reinjection in order to remove infiltration liquid and associated toxic molecules, which in the long term are deleterious for the graft. However, strong centrifugation is not recommended since it leads very quickly to greater adipocyte death. Thus, soft centrifugation (400 g/1 min), preceded by washings, seems to be the most appropriate protocol for the reinjection of adipose tissue. Copyright © 2013 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published

Feasibility of a Short-Arm Centrifuge for Mouse Hypergravity Experiments.

Science.gov (United States)

Morita, Hironobu; Obata, Koji; Abe, Chikara; Shiba, Dai; Shirakawa, Masaki; Kudo, Takashi; Takahashi, Satoru

2015-01-01

To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space. The Japan Aerospace Exploration Agency has developed a novel mouse habitat cage unit that can be installed in the Cell Biology Experiment Facility in the Kibo module of the International Space Station. The Cell Biology Experiment Facility has a short-arm centrifuge to produce artificial 1 g gravity in space for mouse experiments. However, the gravitational gradient formed inside the rearing cage is larger when the radius of gyration is shorter; this may have some impact on mice. Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge. Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination. There was no difference in these responses between mice raised in a short-arm centrifuge and those in a long-arm centrifuge. These results demonstrate the feasibility of using a short-arm centrifuge for mouse experiments.

Spectral analysis of surface waves method to assess shear wave velocity within centrifuge models

Science.gov (United States)

Murillo, Carol Andrea; Thorel, Luc; Caicedo, Bernardo

2009-06-01

The method of the spectral analysis of surface waves (SASW) is tested out on reduced scale centrifuge models, with a specific device, called the mini Falling Weight, developed for this purpose. Tests are performed on layered materials made of a mixture of sand and clay. The shear wave velocity VS determined within the models using the SASW is compared with the laboratory measurements carried out using the bender element test. The results show that the SASW technique applied to centrifuge testing is a relevant method to characterize VS near the surface.

Nonlinear conjugate gradient methods in micromagnetics

Directory of Open Access Journals (Sweden)

J. Fischbacher

2017-04-01

Full Text Available Conjugate gradient methods for energy minimization in micromagnetics are compared. The comparison of analytic results with numerical simulation shows that standard conjugate gradient method may fail to produce correct results. A method that restricts the step length in the line search is introduced, in order to avoid this problem. When the step length in the line search is controlled, conjugate gradient techniques are a fast and reliable way to compute the hysteresis properties of permanent magnets. The method is applied to investigate demagnetizing effects in NdFe12 based permanent magnets. The reduction of the coercive field by demagnetizing effects is μ0ΔH = 1.4 T at 450 K.

Modified conjugate gradient method for diagonalizing large matrices.

Science.gov (United States)

Jie, Quanlin; Liu, Dunhuan

2003-11-01

We present an iterative method to diagonalize large matrices. The basic idea is the same as the conjugate gradient (CG) method, i.e, minimizing the Rayleigh quotient via its gradient and avoiding reintroducing errors to the directions of previous gradients. Each iteration step is to find lowest eigenvector of the matrix in a subspace spanned by the current trial vector and the corresponding gradient of the Rayleigh quotient, as well as some previous trial vectors. The gradient, together with the previous trial vectors, play a similar role as the conjugate gradient of the original CG algorithm. Our numeric tests indicate that this method converges significantly faster than the original CG method. And the computational cost of one iteration step is about the same as the original CG method. It is suitable for first principle calculations.

Approximate error conjugation gradient minimization methods

Science.gov (United States)

Kallman, Jeffrey S

2013-05-21

In one embodiment, a method includes selecting a subset of rays from a set of all rays to use in an error calculation for a constrained conjugate gradient minimization problem, calculating an approximate error using the subset of rays, and calculating a minimum in a conjugate gradient direction based on the approximate error. In another embodiment, a system includes a processor for executing logic, logic for selecting a subset of rays from a set of all rays to use in an error calculation for a constrained conjugate gradient minimization problem, logic for calculating an approximate error using the subset of rays, and logic for calculating a minimum in a conjugate gradient direction based on the approximate error. In other embodiments, computer program products, methods, and systems are described capable of using approximate error in constrained conjugate gradient minimization problems.

Preconditioning the modified conjugate gradient method ...

African Journals Online (AJOL)

In this paper, the convergence analysis of the conventional conjugate Gradient method was reviewed. And the convergence analysis of the modified conjugate Gradient method was analysed with our extension on preconditioning the algorithm. Convergence of the algorithm is a function of the condition number of M-1A.

[Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites].

Science.gov (United States)

Inês, Elizabete De Jesus; Pacheco, Flavia Thamiris Figueiredo; Pinto, Milena Carneiro; Mendes, Patrícia Silva de Almeida; Da Costa-Ribeiro, Hugo; Soares, Neci Matos; Teixeira, Márcia Cristina Aquino

2016-12-01

The diagnosis of intestinal parasitic infections depends on the parasite load, the specific gravity density of the parasite eggs, oocysts or cysts, and the density and viscosity of flotation or sedimentation medium where faeces are processed. To evaluate the concordance between zinc sulphate flotation and centrifugal sedimentation in the recovery of parasites in faecal samples of children. Faecal samples of 330 children from day care centers were evaluated by zinc sulphate flotation and centrifugal sedimentation techniques. The frequencies of detection of parasites by each method were determined and the agreement between the diagnostic techniques was evaluated using the kappa index, with 95% confidence intervals. The faecal flotation in zinc sulphate diagnosed significantly more cases of Trichuris trichiura infection when compared to centrifugal sedimentation (39/330; 11.8% vs. 13/330; 3.9%, p<0.001), with low diagnostic concordance between methods (kappa=0.264; 95% CI: 0.102-0.427). Moreover, all positive samples for Enterobius vermicularis eggs (n=5) and Strongyloides stercoralis larvae (n=3) were diagnosed only by zinc sulphate. No statistical differences were observed between methods for protozoa identification. The results showed that centrifugal flotation in zinc sulphate solution was significantly more likely to detect light helminths eggs such as those of T. trichiura and E. vermicularis in faeces than the centrifugal sedimentation process.

New methods and media for the centrifugation of honey bee (Hymenoptera: Apidae) drone semen.

Science.gov (United States)

Wegener, Jakob; May, Tanja; Kamp, Günter; Bienefeld, Kaspar

2014-02-01

Centrifugation of Apis mellifera L. drone semen is a necessary step in the homogenization of semen pools for the enlargement of the effective breeding population, as well as in the collection of semen by the so-called washing technique. It is also of interest for the removal of cryoprotectants after cryopreservation. The adoption of methods involving semen centrifugation has been hampered by their damaging effect to sperm. Here, we tested four new diluents as well as three additives (catalase, hen egg yolk, and a protease inhibitor), using sperm motility and dual fluorescent staining as indicators of semen quality. Three of the new diluents significantly reduced motility losses after centrifugation, as compared with the literature standard. Values of motility and propidium iodide negativity obtained with two of these diluents were not different from those measured with untreated semen. The least damaging diluent, a citrate-HEPES buffer containing trehalose, was then tested in an insemination experiment with centrifuged semen. Most queens receiving this semen produced normal brood, and the number of sperm reaching the storage organ of the queen was not significantly different from that in queens receiving untreated semen. These results could improve the acceptance of techniques involving the centrifugation of drone semen. The diluent used in the insemination experiment could also serve as semen extender for applications not involving centrifugation.

Influence of recovery method and centrifugation on epididymal sperm from collared peccaries (Pecari tajacu Linnaeus, 1758).

Science.gov (United States)

Bezerra, José Artur Brilhante; da Silva, Andréia Maria; Peixoto, Gislayne Christianne Xavier; da Silva, Mariana de Araújo; Franco de Oliveira, Moacir; Silva, Alexandre Rodrigues

2014-05-01

In order to establish protocols for gamete recovery from accidentally killed wild animals, or to take advantage of those slaughtered by captive breeders, we assess the influence of two methods on the recovery of epididymal sperm from collared peccaries, and verify the effect of centrifugation on such gametes. Genitalia from nine animals were used. For each animal, one epididymis was processed by flotation and the other was processed by retrograde flushing, both using a buffered media based on Tris. Following recovery, sperm were evaluated for motility, vigor, viability, functional membrane integrity, and morphology. A 1-mL aliquot of each sample was centrifuged, the supernatant removed, and the pellet suspended and evaluated as fresh samples. The sperm characteristics did not differ between the samples collected by flotation or retrograde flushing (P methods was affected by centrifugation. In conclusion, epididymal sperm from collared peccaries can be efficiently collected through flotation or retrograde flushing, but not when either is followed by centrifugation.

The optimization of low specific speed centrifugal pump based on incomplete sensitivities

International Nuclear Information System (INIS)

Zhang, R H; Zheng, K; Shi, F X; Yao, L H

2012-01-01

In this research, the optimization method for low specific speed centrifugal pump impeller based on incomplete sensitivities was proposed. The main feature of the algorithm is that it avoids solving the flow field repeatedly in one optimization cycle in finite difference method and it avoids solving the adjoint equation in adjoint method. The blade meridional plan is considered as constant, and the blade camber line was parameterize by Taylor function. The coefficients in the Taylor function were taken as the control variable. The moment acting on the blade was considered as the objective function. With the incomplete sensitivities we can get the gradient of the objective function with respect to the control variable easily, and the blade shape can be renewed according to the inverse direction of the gradient. We will find the optimum design when the objective function is minimized. The computational cost is greatly reduced. The calculation cases show that the proposed theory and method is rotational.

Robust design optimization method for centrifugal impellers under surface roughness uncertainties due to blade fouling

Science.gov (United States)

Ju, Yaping; Zhang, Chuhua

2016-03-01

Blade fouling has been proved to be a great threat to compressor performance in operating stage. The current researches on fouling-induced performance degradations of centrifugal compressors are based mainly on simplified roughness models without taking into account the realistic factors such as spatial non-uniformity and randomness of the fouling-induced surface roughness. Moreover, little attention has been paid to the robust design optimization of centrifugal compressor impellers with considerations of blade fouling. In this paper, a multi-objective robust design optimization method is developed for centrifugal impellers under surface roughness uncertainties due to blade fouling. A three-dimensional surface roughness map is proposed to describe the nonuniformity and randomness of realistic fouling accumulations on blades. To lower computational cost in robust design optimization, the support vector regression (SVR) metamodel is combined with the Monte Carlo simulation (MCS) method to conduct the uncertainty analysis of fouled impeller performance. The analyzed results show that the critical fouled region associated with impeller performance degradations lies at the leading edge of blade tip. The SVR metamodel has been proved to be an efficient and accurate means in the detection of impeller performance variations caused by roughness uncertainties. After design optimization, the robust optimal design is found to be more efficient and less sensitive to fouling uncertainties while maintaining good impeller performance in the clean condition. This research proposes a systematic design optimization method for centrifugal compressors with considerations of blade fouling, providing a practical guidance to the design of advanced centrifugal compressors.

A method for easily customizable gradient gel electrophoresis.

Science.gov (United States)

Miller, Andrew J; Roman, Brandon; Norstrom, Eric

2016-09-15

Gradient polyacrylamide gel electrophoresis is a powerful tool for the resolution of polypeptides by relative mobility. Here, we present a simplified method for generating polyacrylamide gradient gels for routine analysis without the need for specialized mixing equipment. The method allows for easily customizable gradients which can be optimized for specific polypeptide resolution requirements. Moreover, the method eliminates the possibility of buffer cross contamination in mixing equipment, and the time and resources saved with this method in place of traditional gradient mixing, or the purchase of pre-cast gels, are noteworthy given the frequency with which many labs use gradient gel SDS-PAGE. Copyright © 2016 Elsevier Inc. All rights reserved.

Isolation of human monocytes by double gradient centrifugation and their differentiation to macrophages in teflon-coated cell culture bags.

Science.gov (United States)

Menck, Kerstin; Behme, Daniel; Pantke, Mathias; Reiling, Norbert; Binder, Claudia; Pukrop, Tobias; Klemm, Florian

2014-09-09

Human macrophages are involved in a plethora of pathologic processes ranging from infectious diseases to cancer. Thus they pose a valuable tool to understand the underlying mechanisms of these diseases. We therefore present a straightforward protocol for the isolation of human monocytes from buffy coats, followed by a differentiation procedure which results in high macrophage yields. The technique relies mostly on commonly available lab equipment and thus provides a cost and time effective way to obtain large quantities of human macrophages. Briefly, buffy coats from healthy blood donors are subjected to a double density gradient centrifugation to harvest monocytes from the peripheral blood. These monocytes are then cultured in fluorinated ethylene propylene (FEP) Teflon-coated cell culture bags in the presence of macrophage colony-stimulating factor (M-CSF). The differentiated macrophages can be easily harvested and used for subsequent studies and functional assays. Important methods for quality control and validation of the isolation and differentiation steps will be highlighted within the protocol. In summary, the protocol described here enables scientists to routinely and reproducibly isolate human macrophages without the need for cost intensive tools. Furthermore, disease models can be studied in a syngeneic human system circumventing the use of murine macrophages.

A New Optimization Method for Centrifugal Compressors Based on 1D Calculations and Analyses

Directory of Open Access Journals (Sweden)

Pei-Yuan Li

2015-05-01

Full Text Available This paper presents an optimization design method for centrifugal compressors based on one-dimensional calculations and analyses. It consists of two parts: (1 centrifugal compressor geometry optimization based on one-dimensional calculations and (2 matching optimization of the vaned diffuser with an impeller based on the required throat area. A low pressure stage centrifugal compressor in a MW level gas turbine is optimized by this method. One-dimensional calculation results show that D3/D2 is too large in the original design, resulting in the low efficiency of the entire stage. Based on the one-dimensional optimization results, the geometry of the diffuser has been redesigned. The outlet diameter of the vaneless diffuser has been reduced, and the original single stage diffuser has been replaced by a tandem vaned diffuser. After optimization, the entire stage pressure ratio is increased by approximately 4%, and the efficiency is increased by approximately 2%.

Gas Centrifuges and Nuclear Proliferation

Energy Technology Data Exchange (ETDEWEB)

Albright, David

2004-09-15

Gas centrifuges have been an ideal enrichment method for a wide variety of countries. Many countries have built gas centrifuges to make enriched uranium for peaceful nuclear purposes. Other countries have secretly sought centrifuges to make highly enriched uranium for nuclear weapons. In more recent times, several countries have secretly sought or built gas centrifuges in regions of tension. The main countries that have been of interest in the last two decades have been Pakistan, Iraq, Iran, and North Korea. Currently, most attention is focused on Iran, Pakistan, and North Korea. These states did not have the indigenous abilities to make gas centrifuges, focusing instead on illicit and questionable foreign procurement. The presentation covered the following main sections: Spread of centrifuges through illicit procurement; Role of export controls in stopping proliferation; Increasing the transparency of gas centrifuge programs in non-nuclear weapon states; and, Verified dismantlement of gas centrifuge programs. Gas centrifuges are important providers of low enriched uranium for civil nuclear power reactors. They also pose special nuclear proliferation risks. We all have special responsibilities to prevent the spread of gas centrifuges into regions of tension and to mitigate the consequences of their spread into the Middle East, South Asia, and North Asia.

Centrifugal Compressor Aeroelastic Analysis Code

Science.gov (United States)

Keith, Theo G., Jr.; Srivastava, Rakesh

2002-01-01

Centrifugal compressors are very widely used in the turbomachine industry where low mass flow rates are required. Gas turbine engines for tanks, rotorcraft and small jets rely extensively on centrifugal compressors for rugged and compact design. These compressors experience problems related with unsteadiness of flowfields, such as stall flutter, separation at the trailing edge over diffuser guide vanes, tip vortex unsteadiness, etc., leading to rotating stall and surge. Considerable interest exists in small gas turbine engine manufacturers to understand and eventually eliminate the problems related to centrifugal compressors. The geometric complexity of centrifugal compressor blades and the twisting of the blade passages makes the linear methods inapplicable. Advanced computational fluid dynamics (CFD) methods are needed for accurate unsteady aerodynamic and aeroelastic analysis of centrifugal compressors. Most of the current day industrial turbomachines and small aircraft engines are designed with a centrifugal compressor. With such a large customer base and NASA Glenn Research Center being, the lead center for turbomachines, it is important that adequate emphasis be placed on this area as well. Currently, this activity is not supported under any project at NASA Glenn.

Feasibility of a Short-Arm Centrifuge for Mouse Hypergravity Experiments.

Directory of Open Access Journals (Sweden)

Hironobu Morita

Full Text Available To elucidate the pure impact of microgravity on small mammals despite uncontrolled factors that exist in the International Space Station, it is necessary to construct a 1 g environment in space. The Japan Aerospace Exploration Agency has developed a novel mouse habitat cage unit that can be installed in the Cell Biology Experiment Facility in the Kibo module of the International Space Station. The Cell Biology Experiment Facility has a short-arm centrifuge to produce artificial 1 g gravity in space for mouse experiments. However, the gravitational gradient formed inside the rearing cage is larger when the radius of gyration is shorter; this may have some impact on mice. Accordingly, biological responses to hypergravity induced by a short-arm centrifuge were examined and compared with those induced by a long-arm centrifuge. Hypergravity induced a significant Fos expression in the central nervous system, a suppression of body mass growth, an acute and transient reduction in food intake, and impaired vestibulomotor coordination. There was no difference in these responses between mice raised in a short-arm centrifuge and those in a long-arm centrifuge. These results demonstrate the feasibility of using a short-arm centrifuge for mouse experiments.

Semiautomated system for the production and analysis of sucrose density gradients

International Nuclear Information System (INIS)

Lange, C.S.; Liberman, D.F.

1974-01-01

A semiautomated system in DNA damage studies permitting considerable accuracy, speed, and reproducibility in the making and fractionation of sucrose density gradients is described. The system consists of a modified Beckman gradient forming device that makes six gradients simultaneously and delivers them into six 12.5 ml polyallomer centrifuge tubes in such a manner that new material is continuously added to the meniscus of the gradient. The gradients are fractionated three at a time and up to 100 fractions per gradient can be collected automatically directly into scintillation vials with a choice of drop counting or time mode with rinse and automatic addition of scintillation fluid to each vial. The system can process up to six gradients per hour but centrifugation time is usually the limiting factor. With neutral sucrose gradients, sharp, reproducible, monodisperse peaks containing up to 100 percent of the gradient radioactivity are usually obtained but a smaller monodisperse peak containing as little as 3.5 percent of the gradient radioactivity can be detected under conditions where some pairs of molecules might tangle or dimerize. The resolution and reproducibility of this system when used with neutral sucrose gradients is at least the equal if not superior to that commonly claimed for alkaline sucrose gradients. (U.S.)

Observations on centrifugation: application to centrifuge development.

Science.gov (United States)

Roberts, T; Smith, M; Roberts, B

1999-11-01

This report outlines the background to the development of an automated, serial, discrete centrifuge, reporting on the criteria considered essential in such an instrument. We established the criteria by examining the detailed logistics of centrifuge operation in a hospital laboratory. The mean sample load per run, using six centrifuges, was 13.6 samples, and the user-selectable cycle time ranged from 00:01:10 to 00:12:33 (hours:minutes:seconds) with a fixed g value of 1050. During the laboratory working window, (0900-1700), only 50% of the centrifuge capacity was utilized and more than one-third of the sample workload was delayed for >5 min because the centrifuges were not emptied promptly. In addition, 35% of the sample workload was centrifuged for less than the time prescribed in the operational specifications. Based on these findings, we designed a new continuous, serial centrifuge to overcome some of the deficiencies noted in the logistics study. The centrifuge operates continuously, nominally treating 150 samples/h, with a cycle time of 5 min at 1,000 g. The cycle time and g value are variable between limits, and their selection governs the throughput rate. Each sample is centrifuged separately in individual rotors mounted in a sturdy carousel with a periphery that traverses a load/unload station. There is no sample delay because of operator absence, and the capacity is fully utilized. The centrifuge can operate in a stand-alone capacity or has the capability of being integrated into a sample preparation system or as a direct front end for high-throughput analyzers.

Spectral analysis of surface waves method to assess shear wave velocity within centrifuge models

OpenAIRE

MURILLO, Carol Andrea; THOREL, Luc; CAICEDO, Bernardo

2009-01-01

The method of the spectral analysis of surface waves (SASW) is tested out on reduced scale centrifuge models, with a specific device, called the mini Falling Weight, developed for this purpose. Tests are performed on layered materials made of a mixture of sand and clay. The shear wave velocity VS determined within the models using the SASW is compared with the laboratory measurements carried out using the bender element test. The results show that the SASW technique applied to centrifuge test...

Comparison of genetic algorithms with conjugate gradient methods

Science.gov (United States)

Bosworth, J. L.; Foo, N. Y.; Zeigler, B. P.

1972-01-01

Genetic algorithms for mathematical function optimization are modeled on search strategies employed in natural adaptation. Comparisons of genetic algorithms with conjugate gradient methods, which were made on an IBM 1800 digital computer, show that genetic algorithms display superior performance over gradient methods for functions which are poorly behaved mathematically, for multimodal functions, and for functions obscured by additive random noise. Genetic methods offer performance comparable to gradient methods for many of the standard functions.

CENTRIFUGE APPARATUS

Science.gov (United States)

Skarstrom, C.; Urey, H.C.; Cohen, K.

1960-08-01

A high-speed centrifuge for the separation of gaseous isotopes is designed comprising a centrifugal pump mounted on the outlet of a centrifuge bowl and arranged to pump the heavy and light fractions out of the centrifuge bowl in two separate streams.

Flow in a centrifugal fan impeller at off-design conditions

Science.gov (United States)

Wright, T.; Tzou, K. T. S.; Madhavan, S.

1984-06-01

A fully three-dimensional finite element analysis of inviscid, incompressible blade channel flow is the basis of the present study of both predicted and measured surface velocity and pressure distributions in the internal flow channels of a centrifugal fan impeller, for volume flow rates of 80-125 percent the design flow rate. The experimental results made extensive use of blade and sidewall surface pressure taps installed in a scale model of an airfoil-bladed centrifugal fan impeller. The results obtained illustrate the ability of both flow analyses to predict the dominant features of the impeller flow field, including peak blade surface velocities and adverse gradients at flows far from the design point. Insight is also gained into the limiting channel diffusion values for typical centrifugal cascade performance, together with the influence of viscous effects, as seen in deviations from ideal flow predictions.

Steady state behavior of rotating plasmas in a vacuum-arc centrifuge

International Nuclear Information System (INIS)

Bittencourt, J.A.; Ludwig, G.O.

1987-01-01

The steady state behaviour of the fully ionized, multiple species, rotating, magnetized plasma in a vacuum-arc plasma centrifuge is described in detail. The analysis is based on a multiple species fluid model which includes electromagnetic, pressure gradient, centrifugal and collisional forces, for each species, in cylindrical geometry. It is shown that there is a family of theoretically possible dynamical equilibrium configurations, which can be achieved by different combinations of ion rotation velocity, radial ion density distribution and radial dependence of internal electric potential. The parametric dependences of the various plasma parameters under equilibrium conditions, including the ion separation factor, are presented for a nickel-copper plasma. The numerical results are analysed and discussed in light of experimentally measured plasma characteristics in a vacuum-arc plasma centrifuge. (author)

Steady state behavior of rotating plasmas in a vacuum-arc centrifuge

International Nuclear Information System (INIS)

Bittencourt, J.A.; Ludwig, G.O.

1986-06-01

The steady state behavior of the fully ionized, multiple species, rotating, magnetized plasma in a vacuum-arc plasma centrifuge is described in detail. The analysis is based on a multiple species fluid model which includes electromagnetic, pressure gradient, centrifugal and collisional forces, for each species, in cylindrical geometry. It is showm that there is a family of theoretically possible dynamical equilibrium configurations, which can be achieved by different combinations of ion rotation velocity, radial ion density distribution and radial dependence of internal electric potential. The parametric dependences of the various plasma parameters under equilibrium conditions, including the ion separation factor, are presented for a nickel-copper plasma. The numerical results are analysed and discussed on light of experimentally measured plasma characteristics in a vacuum-arc plasma centrifuge. (Author) [pt

Influences of centrifugation on cells and tissues in liposuction aspirates: optimized centrifugation for lipotransfer and cell isolation.

Science.gov (United States)

Kurita, Masakazu; Matsumoto, Daisuke; Shigeura, Tomokuni; Sato, Katsujiro; Gonda, Koichi; Harii, Kiyonori; Yoshimura, Kotaro

2008-03-01

Although injective autologous fat transplantation is one of the most attractive options for soft-tissue augmentation, problems such as unpredictability and fibrosis resulting from fat necrosis limit its universal acceptance. Centrifugation is one of most common methods for overcoming these difficulties. This study was performed to investigate quantitatively the effects of centrifugation on liposuction aspirates to optimize centrifugal conditions for fat transplantation and isolation of adipose-derived stem cells. Liposuction aspirates, obtained from eight healthy female donors, were either not centrifuged or centrifuged at 400, 700, 1200, 3000, or 4200 g for 3 minutes. The volumes of the oil, adipose, and fluid portions and numbers of blood cells and adipose-derived cells in each portion were examined. The processed adipose tissues (1 ml) were injected into athymic mice, and grafts were harvested and weighed at 4 weeks. Morphologic alterations were observed using light and scanning electron microscopy. Centrifugation concentrated adipose tissues and adipose-derived stem cells in the adipose portion and partly removed red blood cells from the adipose portion. Centrifugation at more than 3000 g significantly damaged adipose-derived stem cells. Centrifugation enhanced graft take per 1 ml centrifuged adipose but reduced calculated graft take per 1 ml adipose before centrifugation. Excessive centrifugation can destroy adipocytes and adipose-derived stem cells, but appropriate centrifugation concentrates them, resulting in enhanced graft take. The authors tentatively recommend 1200 g as an optimized centrifugal force for obtaining good short- and long-term results in adipose transplantation.

CFD simulation of centrifugal cells washers.

Science.gov (United States)

Kellet, Beth E; Binbing, Han; Dandy, David S; Wickramasinghe, S Ranil

2004-01-01

The feasibility of using computational fluid dynamics to guide the design of better centrifuges for processing shed blood is explored here. The velocity field and the rate of protein removal from the shed blood have been studied. The results indicate that computational fluid dynamics could help screen preliminary centrifuge bowl designs thus reducing the number of initial experimental tests required when developing new centrifuge bowls. Though the focus of this work is on washing shed blood the methods developed here are applicable to the design of centrifuge bowls for other blood processing applications.

Shape optimization of high power centrifugal compressor using multi-objective optimal method

Energy Technology Data Exchange (ETDEWEB)

Kang, Hyun Soo; Lee, Jeong Min; Kim, Youn Jea [School of Mechanical Engineering, Sungkyunkwan University, Seoul (Korea, Republic of)

2015-03-15

In this study, a method for optimal design of impeller and diffuser blades in the centrifugal compressor using response surface method (RSM) and multi-objective genetic algorithm (MOGA) was evaluated. A numerical simulation was conducted using ANSYS CFX with various values of impeller and diffuser parameters, which consist of leading edge (LE) angle, trailing edge (TE) angle, and blade thickness. Each of the parameters was divided into three levels. A total of 45 design points were planned using central composite design (CCD), which is one of the design of experiment (DOE) techniques. Response surfaces that were generated on the basis of the results of DOE were used to determine the optimal shape of impeller and diffuser blade. The entire process of optimization was conducted using ANSYS Design Xplorer (DX). Through the optimization, isentropic efficiency and pressure recovery coefficient, which are the main performance parameters of the centrifugal compressor, were increased by 0.3 and 5, respectively.

Shape optimization of high power centrifugal compressor using multi-objective optimal method

International Nuclear Information System (INIS)

Kang, Hyun Soo; Lee, Jeong Min; Kim, Youn Jea

2015-01-01

In this study, a method for optimal design of impeller and diffuser blades in the centrifugal compressor using response surface method (RSM) and multi-objective genetic algorithm (MOGA) was evaluated. A numerical simulation was conducted using ANSYS CFX with various values of impeller and diffuser parameters, which consist of leading edge (LE) angle, trailing edge (TE) angle, and blade thickness. Each of the parameters was divided into three levels. A total of 45 design points were planned using central composite design (CCD), which is one of the design of experiment (DOE) techniques. Response surfaces that were generated on the basis of the results of DOE were used to determine the optimal shape of impeller and diffuser blade. The entire process of optimization was conducted using ANSYS Design Xplorer (DX). Through the optimization, isentropic efficiency and pressure recovery coefficient, which are the main performance parameters of the centrifugal compressor, were increased by 0.3 and 5, respectively

Kinetically limited differential centrifugation as an inexpensive and readily available alternative to centrifugal elutriation.

Science.gov (United States)

Tan, Jinwang; Lee, Byung-Doo; Polo-Parada, Luis; Sengupta, Shramik

2012-08-01

When separating two species with similar densities but differing sedimentation velocities (because of differences in size), centrifugal elutriation is generally the method of choice. However, a major drawback to this approach is the requirement for specialized equipment. Here, we present a new method that achieves similar separations using standard benchtop centrifuges by loading the seperands as a layer on top of a dense buffer of a specified length, and running the benchtop centrifugation process for a calculated amount of time, thereby ensuring that all faster moving species are collected at the bottom, while all slower moving species remain in the buffer. We demonstrate the use of our procedure to isolate bacteria from blood culture broth (a mixture of bacterial growth media, blood, and bacteria).

Percoll gradient-centrifuged capacitated mouse sperm have increased fertilizing ability and higher contents of sulfogalactosylglycerolipid and docosahexaenoic acid-containing phosphatidylcholine compared to washed capacitated mouse sperm.

Science.gov (United States)

Furimsky, Anna; Vuong, Ngoc; Xu, Hongbin; Kumarathasan, Premkumari; Xu, Min; Weerachatyanukul, Wattana; Bou Khalil, Maroun; Kates, Morris; Tanphaichitr, Nongnuj

2005-03-01

Although Percoll gradient centrifugation has been used routinely to prepare motile human sperm, its use in preparing motile mouse sperm has been limited. Here, we showed that Percoll gradient-centrifuged (PGC) capacitated mouse sperm had markedly higher fertilizing ability (sperm-zona pellucida [ZP] binding and in vitro fertilization) than washed capacitated mouse sperm. We also showed that the lipid profiles of PGC capacitated sperm and washed capacitated sperm differed significantly. The PGC sperm had much lower contents of cholesterol and phospholipids. This resulted in relative enrichment of male germ cell-specific sulfogalactosylglycerolipid (SGG), a ZP-binding ligand, in PGC capacitated sperm, and this would explain, in part, their increased ZP-binding ability compared with that of washed capacitated sperm. Analyses of phospholipid fatty acyl chains revealed that PGC capacitated sperm were enriched in phosphatidylcholine (PC) molecular species containing highly unsaturated fatty acids (HUFAs), with docosahexaenoic acid (DHA; C22: 6n-3) being the predominant HUFA (42% of total hydrocarbon chains of PC). In contrast, the level of PC-HUFAs comprising arachidonic acid (20:4n-6), docosapentaenoic acid (C22:5n-6), and DHA in washed capacitated sperm was only 27%. Having the highest unsaturation degree among all HUFAs in PC, DHA would enhance membrane fluidity to the uppermost. Therefore, membranes of PGC capacitated sperm would undergo fertilization-related fusion events at higher rates than washed capacitated sperm. These results suggested that PGC mouse sperm should be used in fertilization experiments and that SGG and DHA should be considered to be important biomarkers for sperm fertilizing ability.

Particle attraction effects on the centrifugal casting and extrusion of alumina

International Nuclear Information System (INIS)

Schilling, C.H.; Bergstroem, L.; Ker, H.L.; Aksay, I.A.

1993-01-01

Interparticle attraction forces were empirically related to the centrifugal casting and extrusion behavior of flocculated alumina suspensions. Attractive forces were altered by two approaches: Salt flocculation, which entails regulating the electrical double-layer thickness through electrolyte additions; and screening of van der Waals attraction by steric interactions of surface-adsorbed fatty acids. Specimens produced by both compressibility at a critical maximum density that increased with decreasing interparticle attraction. Gradients in packing density during centrifugal casting were alleviated using both methods as long as spatial variations of the effective stress were within the low-compressibility range. We hypothesized that the reduced interparticle attraction in both methods may also raise the threshold packing density at which ductile-to-brittle transitions occur during plastic shear. Specimens prepared with oleic acid adlayers were highly plastic and easily extrudable at solids contents of up to 59 vol%, although salt-flocculated samples at 55 vol% density extruded at creeping flow rates that were insensitive to the applied pressure. Results suggested that particle rearrangement during shear, is a rate-limiting process, with an average relaxation time that is lowered by reducing interparticle attraction

Research on the development of the centrifugal spinning

Directory of Open Access Journals (Sweden)

Zhang Zhiming

2017-01-01

Full Text Available Centrifugal spinning is a new and efficient method to produce nanofibers quickly. It makes use of the centrifugal force instead of high voltage to produce the nanofibers. The centrifugal spinning has many advantages such as no high voltage, high yield, simple structure, no pollution and can be applied to high polymer material, ceramic and metal material. In order to have more understand about this novel nanofibers formation method, this paper introduces the method of centrifugal spinning and the effect of rotation speed, the properties of material such as viscosity and solvent evaporation, collector distance which have an impact on nanofibers morphology and diameter were also analyzed.

Plasma centrifuge

International Nuclear Information System (INIS)

Ikehata, Takashi; Mase, Hiroshi

1998-01-01

The plasma centrifuge is one of statistical isotope separation processes which uses the centrifugal force of a J x B driven rotating plasma in a magnetic field to give rise to the mass-dependent radial transport of isotopic ions. The system has been developed as an alternative to the gas centrifuge because a much higher rotational velocity and separation factor have been achieved. In this review, the physical aspects of the plasma centrifuge followed by the recent experimental achievements are described, especially in comparison with the gas centrifuge. (author)

Extraction of soil solution by drainage centrifugation-effects of centrifugal force and time of centrifugation on soil moisture recovery and solute concentration in soil moisture of loess subsoils.

Science.gov (United States)

Fraters, Dico; Boom, Gerard J F L; Boumans, Leo J M; de Weerd, Henk; Wolters, Monique

2017-02-01

The solute concentration in the subsoil beneath the root zone is an important parameter for leaching assessment. Drainage centrifugation is considered a simple and straightforward method of determining soil solution chemistry. Although several studies have been carried out to determine whether this method is robust, hardly any results are available for loess subsoils. To study the effect of centrifugation conditions on soil moisture recovery and solute concentration, we sampled the subsoil (1.5-3.0 m depth) at commercial farms in the loess region of the Netherlands. The effect of time (20, 35, 60, 120 and 240 min) on recovery was studied at two levels of the relative centrifugal force (733 and 6597g). The effect of force on recovery was studied by centrifugation for 35 min at 117, 264, 733, 2932, 6597 and 14,191g. All soil moisture samples were chemically analysed. This study shows that drainage centrifugation offers a robust, reproducible and standardised way for determining solute concentrations in mobile soil moisture in silt loam subsoils. The centrifugal force, rather than centrifugation time, has a major effect on recovery. The maximum recovery for silt loams at field capacity is about 40%. Concentrations of most solutes are fairly constant with an increasing recovery, as most solutes, including nitrate, did not show a change in concentration with an increasing recovery.

Sperm Na+, K+-ATPase and Ca2+-ATPase activity: A preliminary study of comparison of swim up and density gradient centrifugation methods for sperm preparation

Science.gov (United States)

Lestari, Silvia W.; Larasati, Manggiasih D.; Asmarinah, Mansur, Indra G.

2018-02-01

As one of the treatment for infertility, the success rate of Intrauterine Insemination (IUI) is still relatively low. Several sperm preparation methods, swim-up (SU) and the density-gradient centrifugation (DGC) are frequently used to select for better sperm quality which also contribute to IUI failure. Sperm selection methods mainly separate the motile from the immotile sperm, eliminating the seminal plasma. The sperm motility involves the structure and function of sperm membrane in maintaining the balance of ion transport system which is regulated by the Na+, K+-ATPase, and Ca2+-ATPase enzymes. This study aims to re-evaluate the efficiency of these methods in selecting for sperm before being used for IUI and based the evaluation on sperm Na+,K+-ATPase and Ca2+-ATPase activities. Fourteen infertile men from couples who underwent IUI were involved in this study. The SU and DGC methods were used for the sperm preparation. Semen analysis was performed based on the reference value of World Health Organization (WHO) 2010. After isolating the membrane fraction of sperms, the Na+, K+-ATPase activity was defined as the difference in the released inorganic phosphate (Pi) with and without the existence of 10 mM ouabain in the reaction, while the Ca2+-ATPase was determined as the difference in Pi contents with and without the existence of 55 µm CaCl2. The prepared sperm demonstrated a higher percentage of motile sperm compared to sperm from the whole semen. Additionally, the percentage of motile sperm of post-DGC showed higher result than the sperm from post-SU. The velocity of sperm showed similar pattern with the percentage of motile sperm, in which the velocity of prepared sperm was higher than the sperm from whole semen. Furthermore, the sperm velocity of post-DGC was higher compared to the sperm from post-SU. The Na+, K+-ATPase activity of prepared sperm was higher compared to whole semen, whereas Na+, K+-ATPase activity in the post DGC was higher than post SU. The Ca2

A direct method for determining complete positive and negative capillary pressure curves for reservoir rock using the centrifuge

Energy Technology Data Exchange (ETDEWEB)

Spinler, E.A.; Baldwin, B.A. [Phillips Petroleum Co., Bartlesville, OK (United States)

1997-08-01

A method is being developed for direct experimental determination of capillary pressure curves from saturation distributions produced during centrifuging fluids in a rock plug. A free water level is positioned along the length of the plugs to enable simultaneous determination of both positive and negative capillary pressures. Octadecane as the oil phase is solidified by temperature reduction while centrifuging to prevent fluid redistribution upon removal from the centrifuge. The water saturation is then measured via magnetic resonance imaging. The saturation profile within the plug and the calculation of pressures for each point of the saturation profile allows for a complete capillary pressure curve to be determined from one experiment. Centrifuging under oil with a free water level into a 100 percent water saturated plug results in the development of a primary drainage capillary pressure curve. Centrifuging similarly at an initial water saturation in the plug results in the development of an imbibition capillary pressure curve. Examples of these measurements are presented for Berea sandstone and chalk rocks.

Separative properties of counter-current beams type centrifuge, (2)

International Nuclear Information System (INIS)

Todo, Fukuzo

1975-01-01

One-time through scheme is studied, which would produce the highest overall centrifuge efficiency among the three different flow schemes of enriching, stripping and one-time through. If the ''optimum concentration method'' is applied to the one-time through centrifuge, the machine will be able to obtain a very high efficiency at small gas flow rates. A proposed arrangement of centrifuges for this method is shown. The efficiency of this method will be more than 15--20% higher than obtainable with enriching scheme. When the radial gas flow rate near the end caps in the rotor is increased to about 10% of the total gas feed rate, the efficiency was found to decrease by only 1%. The efficiency appears to be almost independent of small amounts of refluxing gas flow. Since a separation method having a high efficiency at small gas flow rates is required for large-scale gas centrifuge plants, the one-time through centrifuge is promising, provided the optimum concentration method is adopted. (auth.)

Use of short-radius centrifugation to augment ankle-brachial indices.

Science.gov (United States)

Grenon, S Marlene; Mateus, Jaime; Hsiang, York; Sidhu, Ravi; Young, Laurence; Gagnon, Joel

2009-06-01

Peripheral arterial disease is mainly caused by atherosclerosis and is characterized by decreased circulation, lower blood pressure, and insufficient tissue perfusion in the lower extremities. The hemodynamics of standing and altered gravity environments have been well studied relative to arm blood pressures but are less well understood for ankle pressures. Because regional blood pressure depends, in part, on the gravitational pressure gradient, we hypothesized that artificial gravity exposure on a short-arm centrifuge with the center of rotation above the head would increase blood pressure in the lower extremities. Cardiovascular parameters for 12 healthy subjects were measured during exposure to supine short-arm centrifugation at 20, 25, and 30 revolutions per minute (rpm), corresponding to centripetal accelerations of 0.94, 1.47, and 2.11 Gz at the foot level, respectively. Systolic ankle blood pressure significantly increased at all levels of centrifugation. Ankle-brachial indices (the ratio of systolic ankle to arm blood pressures) increased significantly from 1.17 +/- 0.03 to 1.58 +/- 0.03 at 0.94 Gz (P blood pressure significantly increased at 2.11 Gz, but heart rate did not change significantly. All parameters returned to normal after cessation of centrifugation. We demonstrated that short-radius centrifugation leads to an increase in ankle-brachial indices. This could have potential implications for the treatment of peripheral arterial disease.

Engineering design of centrifugal casting machine

Science.gov (United States)

Kusnowo, Roni; Gunara, Sophiadi

2017-06-01

Centrifugal casting is a metal casting process in which metal liquid is poured into a rotating mold at a specific temperature. Given round will generate a centrifugal force that will affect the outcome of the casting. Casting method is suitable in the manufacture of the casting cylinder to obtain better results. This research was performed to design a prototype machine by using the concept of centrifugal casting. The design method was a step-by-step systematic approach in the process of thinking to achieve the desired goal of realizing the idea and build bridges between idea and the product. Design process was commenced by the conceptual design phase and followed by the embodiment design stage and detailed design stage. With an engineering design process based on the method developed by G. E. Dieter, draft prototype of centrifugal casting machine with dimension of 550×450×400 mm, ¼ HP motor power, pulley and belt mechanism, diameter of 120-150mm, simultaneously with the characteristics of simple casting product, easy manufacture and maintenance, and relatively inexpensive, was generated.

The multigrid preconditioned conjugate gradient method

Science.gov (United States)

Tatebe, Osamu

1993-01-01

A multigrid preconditioned conjugate gradient method (MGCG method), which uses the multigrid method as a preconditioner of the PCG method, is proposed. The multigrid method has inherent high parallelism and improves convergence of long wavelength components, which is important in iterative methods. By using this method as a preconditioner of the PCG method, an efficient method with high parallelism and fast convergence is obtained. First, it is considered a necessary condition of the multigrid preconditioner in order to satisfy requirements of a preconditioner of the PCG method. Next numerical experiments show a behavior of the MGCG method and that the MGCG method is superior to both the ICCG method and the multigrid method in point of fast convergence and high parallelism. This fast convergence is understood in terms of the eigenvalue analysis of the preconditioned matrix. From this observation of the multigrid preconditioner, it is realized that the MGCG method converges in very few iterations and the multigrid preconditioner is a desirable preconditioner of the conjugate gradient method.

Centrifugal pumps

CERN Document Server

Anderson, HH

1981-01-01

Centrifugal Pumps describes the whole range of the centrifugal pump (mixed flow and axial flow pumps are dealt with more briefly), with emphasis on the development of the boiler feed pump. Organized into 46 chapters, this book discusses the general hydrodynamic principles, performance, dimensions, type number, flow, and efficiency of centrifugal pumps. This text also explains the pumps performance; entry conditions and cavitation; speed and dimensions for a given duty; and losses. Some chapters further describe centrifugal pump mechanical design, installation, monitoring, and maintenance. The

Quantification of platelets obtained by different centrifugation protocols in SHR rats

OpenAIRE

João Alberto Yazigi Junior; João Baptista Gomes dos Santos; Bruno Rodrigues Xavier; Marcela Fernandes; Sandra Gomes Valente; Vilnei Mattiolli Leite

2015-01-01

ABSTRACT OBJECTIVE: To quantify the platelet concentration in the blood of SHR rats, by means of different centrifugation protocols, and to evaluate what the most effective method for obtaining platelets is. METHODS: We used 40 male rats of the isogenic SHR lineage. The animals were divided into three groups: control, using whole blood without centrifugation; single centrifugation, using whole blood subjected to a single centrifugation at 200 × gand 400 × g; and double centrifugation, usin...

Application of the conjugate-gradient method to ground-water models

Science.gov (United States)

Manteuffel, T.A.; Grove, D.B.; Konikow, Leonard F.

1984-01-01

The conjugate-gradient method can solve efficiently and accurately finite-difference approximations to the ground-water flow equation. An aquifer-simulation model using the conjugate-gradient method was applied to a problem of ground-water flow in an alluvial aquifer at the Rocky Mountain Arsenal, Denver, Colorado. For this application, the accuracy and efficiency of the conjugate-gradient method compared favorably with other available methods for steady-state flow. However, its efficiency relative to other available methods depends on the nature of the specific problem. The main advantage of the conjugate-gradient method is that it does not require the use of iteration parameters, thereby eliminating this partly subjective procedure. (USGS)

Discrete gradient methods for solving variational image regularisation models

International Nuclear Information System (INIS)

Grimm, V; McLachlan, Robert I; McLaren, David I; Quispel, G R W; Schönlieb, C-B

2017-01-01

Discrete gradient methods are well-known methods of geometric numerical integration, which preserve the dissipation of gradient systems. In this paper we show that this property of discrete gradient methods can be interesting in the context of variational models for image processing, that is where the processed image is computed as a minimiser of an energy functional. Numerical schemes for computing minimisers of such energies are desired to inherit the dissipative property of the gradient system associated to the energy and consequently guarantee a monotonic decrease of the energy along iterations, avoiding situations in which more computational work might lead to less optimal solutions. Under appropriate smoothness assumptions on the energy functional we prove that discrete gradient methods guarantee a monotonic decrease of the energy towards stationary states, and we promote their use in image processing by exhibiting experiments with convex and non-convex variational models for image deblurring, denoising, and inpainting. (paper)

Comparison of the different methods for the recovery of suspended matter from estuarine waters: deposition, filtration and centrifugation

Energy Technology Data Exchange (ETDEWEB)

Etcheber, H; Jouanneau, J M

1980-12-01

The advantages and disadvantages of three methods used to recover heavy metals from estuarine waters are compared. Deposition, filtration, and centrifugation were used on samples from the Gironde estuary of France. Recovery by deposition is satisfactory in cases of flocculation and high turbidity. Centrifugation is reliable for the determination of several elements in zones of low turbidity. (12 references, 4 tables)

Gradient-type methods in inverse parabolic problems

International Nuclear Information System (INIS)

Kabanikhin, Sergey; Penenko, Aleksey

2008-01-01

This article is devoted to gradient-based methods for inverse parabolic problems. In the first part, we present a priori convergence theorems based on the conditional stability estimates for linear inverse problems. These theorems are applied to backwards parabolic problem and sideways parabolic problem. The convergence conditions obtained coincide with sourcewise representability in the self-adjoint backwards parabolic case but they differ in the sideways case. In the second part, a variational approach is formulated for a coefficient identification problem. Using adjoint equations, a formal gradient of an objective functional is constructed. A numerical test illustrates the performance of conjugate gradient algorithm with the formal gradient.

Separations by centrifugal phenomena

International Nuclear Information System (INIS)

Hsu, H.W.

1981-01-01

The technical information presented herein emphasizes the uniqueness of the centrifugal separations methodology and pertinent theory for various kinds of centrifugation. The topics are arranged according to gas, liquid, and solid phases, in the order of increasing densities. Much space is devoted to liquid centrifugation because of the importance of this technique in chemical and biological laboratories. Many separational and characterizational examples are illustrated in detail. The material has been divided into 7 chapters entitled: 1) Introduction, 2) Basic Theory of Centrifugation, 3) Gas Centrifuges, 4) Preparative Liquid Centrifuges, 5) Analytical Liquid Centrifuges, 6) Liquid Centrifuges in Practice, and 7) Mechanical Separations by Centrifuges. Separate abstracts have been prepared for each chapter except the introduction

Formation of thin film like assembly of exfoliated C3N4 nanoflakes by solvent non-evaporative method using centrifuge

Science.gov (United States)

Tejasvi, Ravi; Basu, Suddhasatwa

2017-12-01

A simple method for depositing a thin film of nanomaterial on a substrate using centrifugation technique has been developed, whereby solvent evaporation is prevented and solvent reuse is possible. The centrifuge technique of deposition yields uniform, smooth thin film irrespective of substrate surface texture. The deposited TiO2/eC3N4 film studied, through field emission scanning electron microscope, atomic force microscope, and optical surface profilometer, shows variation in surface roughness on the basis of centrifugation speeds. Initially film coverage improves and surface roughness decreases with the increase in rpm of the centrifuge and the surface roughness slightly increases with further increase in rpm. The photoelectrochemical studies of TiO2/eC3N4 films suggest that the centrifuge technique forms better heterojunctions compared to that by spin coating technique leading to enhanced photoelectrochemical water splitting.

First Birth after Sperm Selection through Discontinuous Gradient Centrifugation and Artificial Insemination from a Chromosomal Translocation Carrier

Directory of Open Access Journals (Sweden)

Alexandre Rouen

2014-01-01

Full Text Available Introduction. Balanced chromosomal carriers, though usually healthy, are confronted with recurrent spontaneous abortions and malformations in the offspring. Those are related to the transmission of an abnormal, chromosomally unbalanced genotype. We evidenced that the proportion of unbalanced spermatozoa can be significantly decreased through a sperm preparation process called discontinuous gradient centrifugation (DGC. We therefore started offering intrauterine inseminations with this procedure to couples with a male translocation carriers. Case Presentation. We report the case of a 37-year-old man carrying a t(3;10(q25;p13 reciprocal translocation. He and his partner had had trouble conceiving for ten years and had four spontaneous abortions. DGC in this patient decreased the proportion of unbalanced spermatozoa from 63.6% to 52.3%. They were therefore offered intrauterine insemination with DGC, which eventually led to the birth of a healthy female child carrying the paternal translocation. Conclusion. We showed that translocation carriers could be offered intrauterine inseminations with DGC. Before this, the only two options were natural conception with prenatal diagnosis and termination of chromosomally unbalanced fetuses or preimplantation genetic diagnosis, which is a much heavier and costly procedure. We are currently offering this option through a multicentric program in France, and this is the first birth originating from it.

Sperm quality after swim up and density gradient centrifugation sperm preparation with supplementation of alpha lipoic acid (ALA): A preliminary study

Science.gov (United States)

Lestari, Silvia W.; Lestari, Sarah H.; Pujianto, Dwi A.

2018-02-01

Intra uterine insemination (IUI) as one of the treatment for infertility, persists low success rate. A factor that contributes to the unsuccessful of IUI is sperm preparation, performed through Swim-up (SU) and Density Gradient Centrifugation (DGC) methods. Furthermore, studies have shown that Alpha Lipoic Acid (ALA) is a potent antioxidant that could enhance the sperm motility and protect the DNA integrity of the sperm [1]. This study is aimed to re-evaluate the efficiency of the DGC and SU methods in selecting sperm before being transferred for IUI by the supplementation of ALA based on the sperm DNA integrity. Semen samples were obtained from 13 men from partners of women who are infertile (normozoospermia) and underwent IUI. Semen analysis based on the guideline of World Health Organization (WHO) 2010 was performed to measure the sperm motility and velocity, before and after sperm preparation. Then, samples were incubated with Alpha Lipoic Acid (ALA) in 0.625 mg (ALA 1), 1.25 mg (ALA 2) and 2.5 mg (ALA 3). The Sperm Chromatin Dispersion (SCD) test was performed to evaluate the sperm DNA Fragmentation Index (DFI). The percentage of motile sperm was higher in prepared sperm (post-DGC and post-SU) than in whole semen. Furthermore, the percentage of motile sperm was higher in post-DGC compared to post-SU. The level of DFI after the supplementation of ALA was decreased in prepared sperm compared to the whole semen. ALA was proved capable to select the better sperm quality with decreased sperm DNA fragmentation of prepared sperm in the all of DFI category.

Description of a double centrifugation tube method for concentrating canine platelets

OpenAIRE

Perazzi, Anna; Busetto, Roberto; Martinello, Tiziana; Drigo, Michele; Pasotto, Daniela; Cian, Francesco; Patruno, Marco; Iacopetti, Ilaria

2013-01-01

Background To evaluate the efficiency of platelet-rich plasma preparations by means of a double centrifugation tube method to obtain platelet-rich canine plasma at a concentration at least 4 times higher than the baseline value and a concentration of white blood cells not exceeding twice the reference range. A complete blood count was carried out for each sample and each concentrate. Whole blood samples were collected from 12 clinically healthy dogs (consenting blood donors). Blood was proces...

The gas centrifuge, uranium enrichment and nuclear proliferation

International Nuclear Information System (INIS)

Chapman, A.

1988-01-01

The author considers the consequences for controlling nuclear proliferation of the emergence of the gas centrifuge method for enriching uranium and succeeds in the difficult and delicate task of saying enough about gas centrifuge techniques for readers to judge, what may be involved in fully embracing gas centrifuge enrichment within the constraints of an anti-proliferation strategy, whilst at the same time saying nothing that could be construed as encouraging an interest in the gas centrifuge route to highly enriched uranium where none had before existed. (author)

Theory of uranium enrichment by the gas centrifuge

Energy Technology Data Exchange (ETDEWEB)

Olander, D R [California Univ., Berkeley (USA). Lawrence Berkeley Lab.; California Univ., Berkeley (USA). Dept. of Nuclear Engineering)

1981-01-01

Onsager's analysis of the hydrodynamics of fluid circulation in the boundary layer on the rotor wall of a gas centrifuge is reviewed. The description of the flow in the boundary layers on the top and bottom end caps due to Carrier and Maslen is summarized. The method developed by Wood and Morton of coupling the flow models in the rotor wall and end cap boundary layers to complete the hydrodynamic analysis of the centrifuge is presented. Mechanical and thermal methods of driving the internal gas circulation are described. The isotope enrichment which results from the superposition of the elementary separation effect due to the centrifugal field in the gas and its internal circulation is analyzed by the Onsager-Cohen theory. The performance function representing the optimized separative power of a centrifuge as a function of throughput and cut is calculated for several simplified internal flow models. The use of asymmetric ideal cascades to exploit the distinctive features of centrifuge performance functions is illustrated.

Minimum weight protection - Gradient method; Protection de poids minimum - Methode du gradient

Energy Technology Data Exchange (ETDEWEB)

Danon, R.

1958-12-15

After having recalled that, when considering a mobile installation, total weight has a crucial importance, and that, in the case of a nuclear reactor, a non neglectable part of weight is that of protection, this note presents an iterative method which results, for a given protection, to a configuration with a minimum weight. After a description of the problem, the author presents the theoretical formulation of the gradient method as it is applied to the concerned case. This application is then discussed, as well as its validity in terms of convergence and uniqueness. Its actual application is then reported, and possibilities of practical applications are evoked.

Centrifuge modeling of monopiles

DEFF Research Database (Denmark)

Klinkvort, Rasmus Tofte

2010-01-01

To gain a larger knowledge of the monopile foundation concept, centrifuge modeling is used by the geotechnical group at DTU. The centrifuge operated at DTU is a beam centrifuge and was built in 1976. In the recent years it has been upgraded with onboard data acquisition and control systems....... The capabilities of the centrifuge at DTU makes it possible to obtain a scale factor of 85 in experiments which equals a soil volume in prototype scale of a diameter of 40 meters and a depth of 40 meters. This paper describes centrifuge modeling theory, the centrifuge setup at DTU and as an example show results...... from centrifuge tests performed on large diameter piles installed in dry sand....

Comparison of Influenza Virus Particle Purification Using Magnetic Sulfated Cellulose Particles with an Established Centrifugation Method for Analytics.

Science.gov (United States)

Serve, Anja; Pieler, Michael Martin; Benndorf, Dirk; Rapp, Erdmann; Wolff, Michael Werner; Reichl, Udo

2015-11-03

A method for the purification of influenza virus particles using novel magnetic sulfated cellulose particles is presented and compared to an established centrifugation method for analytics. Therefore, purified influenza A virus particles from adherent and suspension MDCK host cell lines were characterized on the protein level with mass spectrometry to compare the viral and residual host cell proteins. Both methods allowed one to identify all 10 influenza A virus proteins, including low-abundance proteins like the matrix protein 2 and nonstructural protein 1, with a similar impurity level of host cell proteins. Compared to the centrifugation method, use of the novel magnetic sulfated cellulose particles reduced the influenza A virus particle purification time from 3.5 h to 30 min before mass spectrometry analysis.

CENTRIFUGE END CAP

Science.gov (United States)

Beams, J.W.; Snoddy, L.B.

1960-08-01

An end cap for ultra-gas centrifuges is designed to impart or remove angular momentum to or from the gas and to bring the entering gas to the temperature of the gas inside the centrifuge. The end cap is provided with slots or fins for adjusting the temperature and the angular momentum of the entering gas to the temperature and momentum of the gas in the centrifuge and is constructed to introduce both the inner and the peripheral stream into the centrifuge.

Centrifugal compressor design for electrically assisted boost

International Nuclear Information System (INIS)

Yang, M Y; Martinez-Botas, R F; Zhuge, W L; Qureshi, U; Richards, B

2013-01-01

Electrically assisted boost is a prominent method to solve the issues of transient lag in turbocharger and remains an optimized operation condition for a compressor due to decoupling from turbine. Usually a centrifugal compressor for gasoline engine boosting is operated at high rotational speed which is beyond the ability of an electric motor in market. In this paper a centrifugal compressor with rotational speed as 120k RPM and pressure ratio as 2.0 is specially developed for electrically assisted boost. A centrifugal compressor including the impeller, vaneless diffuser and the volute is designed by meanline method followed by 3D detailed design. Then CFD method is employed to predict as well as analyse the performance of the design compressor. The results show that the pressure ratio and efficiency at design point is 2.07 and 78% specifically

A Novel Centrifugation Method Using a Cell Salvage Device Offers an Alternative to the Use of Leukocyte-Depleting Filters for Autologous Blood Transfusions.

Science.gov (United States)

Barchilon, Michael; Gaspar, Cristina; Mexas, Angela; Nieter, Don

2016-12-01

Autotransfusion protocols often use the use of costly filters, such as leukocyte-depleting filters (LDFs), to minimize reinfusion of activated leukocytes and inflammatory mediators associated with reperfusion injury (RI). LDFs are used extensively in hospital settings; however, they represent an additional capital expenditure for hospitals, as well as a constraint on the reinfusion rate of blood products for health-care providers. We compared a commonly used LDF to a novel centrifugation method employing a widely used cell salvage device. Complete blood counts and enzyme-linked immunosorbent assays (ELISAs) measuring tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) were performed to compare the efficacy of these methodologies. The LDF removed, on average, 94% of all leukocytes, including 96% of neutrophils. The centrifugation method removed, on average, 89% of all leukocytes, including 91% of neutrophils and resulted in a highly concentrated red blood cell product. Our results suggest both methods offer equivalent leukocyte reduction. TNF-α was also comparably reduced following our novel centrifugation method and the LDF method and IL-2 levels were undetectable in all samples. These results indicate our novel centrifugation method may preclude the need for a LDF during select autotransfusion applications.

Quantification of platelets obtained by different centrifugation protocols in SHR rats

Directory of Open Access Journals (Sweden)

João Alberto Yazigi Junior

2015-12-01

Full Text Available ABSTRACT OBJECTIVE: To quantify the platelet concentration in the blood of SHR rats, by means of different centrifugation protocols, and to evaluate what the most effective method for obtaining platelets is. METHODS: We used 40 male rats of the isogenic SHR lineage. The animals were divided into three groups: control, using whole blood without centrifugation; single centrifugation, using whole blood subjected to a single centrifugation at 200 × gand 400 × g; and double centrifugation, using whole blood subjected one centrifugation at different rotations, followed by collection of whole plasma subjected to another centrifugation at different rotations: 200 × g+ 200 ×g; 200 × g+ 400 × g; 200 × g+ 800 × g; 400 ×g+ 400 × g; 400 × g+ 800 × g. Samples of 3 ml of blood were drawn from each animal by means of cardiac puncture. The blood was stored in Vacutainer collection tubes containing 3.2% sodium citrate. The blood from the control group animals was analyzed without being subjected to centrifugation. After the blood from the other groups of animals had been subjected to centrifugation, the whole plasma was collected and subjected to platelet counting in the lower third of the sample. RESULTS: We obtained greatest platelet enrichment in the subgroup with two centrifugations comprising 400 × gfor 10 min + 400 ×gfor 10 min, in which the mean platelet concentration was 11.30 times higher than that of the control group. CONCLUSION: It was possible to obtain a high platelet concentration using viable simple techniques, by means of centrifugation of whole blood and use of commonly used materials. The most effective method for obtaining platelet concentrate was found in samples subjected to two centrifugations.

Centrifuge enrichment program

International Nuclear Information System (INIS)

Astley, E.R.

1976-01-01

Exxon Nuclear has been active in privately funded research and development of centrifuge enrichment technology since 1972. In October of 1975, Exxon Nuclear submitted a proposal to design, construct, and operate a 3000-MT SWU/yr centrifuge enrichment plant, under the provisions of the proposed Nuclear Fuel Assurance Act of 1975. The U.S. Energy Research and Development Administration (ERDA) accepted the proposal as a basis for negotiation. It was proposed to build a 1000-MT SWU/yr demonstration increment to be operational in 1982; and after successful operation for about one year, expand the facilities into a 3000-MT SWU/yr plant. As part of the overall centrifuge enrichment plant, a dedicated centrifuge manufacturing plant would be constructed; sized to support the full 3000-MT SWU/yr plant. The selection of the centrifuge process by Exxon Nuclear was based on an extremely thorough evaluation of current and projected enrichment technology; results show that the technology is mature and the process will be cost effective. The substantial savings in energy (about 93%) from utilization of the centrifuge option rather than gaseous diffusion is a compelling argument. As part of this program, Exxon Nuclear has a large hardware R and D program, plus a prototype centrifuge manufacturing capability in Malta, New York. To provide a full-scale machine and limited cascade test capability, Exxon Nuclear is constructing a $4,000,000 Centrifuge Test Facility in Richland, Washington. This facility was to initiate operations in the Fall of 1976. Exxon Nuclear is convinced that the centrifuge enrichment process is the rational selection for emergence of a commercial enrichment industry

Xylem vulnerability to cavitation can be accurately characterised in species with long vessels using a centrifuge method.

Science.gov (United States)

Tobin, M F; Pratt, R B; Jacobsen, A L; De Guzman, M E

2013-05-01

Vulnerability to cavitation curves describe the decrease in xylem hydraulic conductivity as xylem pressure declines. Several techniques for constructing vulnerability curves use centrifugal force to induce negative xylem pressure in stem or root segments. Centrifuge vulnerability curves constructed for long-vesselled species have been hypothesised to overestimate xylem vulnerability to cavitation due to increased vulnerability of vessels cut open at stem ends that extend to the middle or entirely through segments. We tested two key predictions of this hypothesis: (i) centrifugation induces greater embolism than dehydration in long-vesselled species, and (ii) the proportion of open vessels changes centrifuge vulnerability curves. Centrifuge and dehydration vulnerability curves were compared for a long- and short-vesselled species. The effect of open vessels was tested in four species by comparing centrifuge vulnerability curves for stems of two lengths. Centrifuge and dehydration vulnerability curves agreed well for the long- and short-vesselled species. Centrifuge vulnerability curves constructed using two stem lengths were similar. Also, the distribution of embolism along the length of centrifuged stems matched the theoretical pressure profile induced by centrifugation. We conclude that vulnerability to cavitation can be accurately characterised with vulnerability curves constructed using a centrifuge technique, even in long-vesselled species. © 2012 German Botanical Society and The Royal Botanical Society of the Netherlands.

New Conjugacy Conditions and Related Nonlinear Conjugate Gradient Methods

International Nuclear Information System (INIS)

Dai, Y.-H.; Liao, L.-Z.

2001-01-01

Conjugate gradient methods are a class of important methods for unconstrained optimization, especially when the dimension is large. This paper proposes a new conjugacy condition, which considers an inexact line search scheme but reduces to the old one if the line search is exact. Based on the new conjugacy condition, two nonlinear conjugate gradient methods are constructed. Convergence analysis for the two methods is provided. Our numerical results show that one of the methods is very efficient for the given test problems

Modern gas centrifuge and rarefied-gas dynamics

International Nuclear Information System (INIS)

Lowry, R.A.; Halle, E.V.; Wood, H.G. III.

1981-01-01

Today, the modern gas centrifuge appears to be the preferred method for the enrichment of the isotopes of uranium on a commercial scale. That this is the case is the result of diligent development programs pursued in this country as well as in the UK, Germany, and the Netherlands over the several decades since the end of WW II. The theoretical modelling of gas centrifuge performance has made notable advances. However, the theoretical work has been based primarily on continuum fluid dynamics considerations. Centrifuge problems involving rarefied gas dynamics considerations are discussed in this paper

Environmental applications of the centrifugal fast analyzer

International Nuclear Information System (INIS)

Goldstein, G.; Strain, J.E.; Bowling, J.L.

1975-12-01

The centrifugal fast analyzer (GeMSAEC Fast Analyzer) was applied to the analysis of pollutants in air and water. Since data acquisition and processing are computer controlled, considerable effort went into devising appropriate software. A modified version of the standard FOCAL interpreter was developed which includes special machine language functions for data timing, acquisition, and storage, and also permits chaining together of programs stored on a disk. Programs were written and experimental procedures developed to implement spectrophotometric, turbidimetric, kinetic (including initial-rate, fixed-time, and variable-time techniques), and chemiluminescence methods of analysis. Analytical methods were developed for the following elements and compounds: SO 2 , O 3 , Ca, Cr, Cu, Fe, Mg, Se(IV), Zn, Cl - , I - , NO 2 - , PO 4 -3 , S -2 , and SO 4 -2 . In many cases, standard methods could be adapted to the centrifugal analyzer, in others new methods were employed. In general, analyses performed with the centrifugal fast analyzer were faster, more precise, and more accurate than with conventional instrumentation

A new method of determining moisture gradient in wood

Science.gov (United States)

Zhiyong Cai

2008-01-01

Moisture gradient in wood and wood composites is one of most important factors that affects both physical stability and mechanical performance. This paper describes a method for measuring moisture gradient in lumber and engineering wood composites as it varies across material thickness. This innovative method employs a collimated radiation beam (x rays or [gamma] rays...

Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production

Directory of Open Access Journals (Sweden)

A.C Lucio

2012-06-01

Full Text Available The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was performed in in vitro produced embryos. The results were validated by the sex ratio of in vitro produced embryos using PCR by Y- specific sequences present in bovine male genomic DNA. After determining genetic sex of in vitro produced embryos, the results showed difference (P<0.05 in deviation of sex ratio when comparing the control group (45.2% females with the other spermatozoa selection procedures (60.6% females (P<0.05. The sperm selection methods are capable of selecting X-bearing spermatozoa without compromising the spermatozoa fertility (cleavage and blastocyst rates, 70% and 26%, respectively and were considered relevant methods to be introduced in bovine in vitro produced embryo programs.

Gradient High Performance Liquid Chromatography Method ...

African Journals Online (AJOL)

Purpose: To develop a gradient high performance liquid chromatography (HPLC) method for the simultaneous determination of phenylephrine (PHE) and ibuprofen (IBU) in solid ..... nimesulide, phenylephrine. Hydrochloride, chlorpheniramine maleate and caffeine anhydrous in pharmaceutical dosage form. Acta Pol.

Comparison of the Lysis Centrifugation Method with the Conventional Blood Culture Method in Cases of Sepsis in a Tertiary Care Hospital

OpenAIRE

Parikh, Harshal R; De, Anuradha S; Baveja, Sujata M

2012-01-01

Introduction : Physicians and microbiologists have long recognized that the presence of living microorganisms in the blood of a patient carries with it considerable morbidity and mortality. Hence, blood cultures have become critically important and frequently performed test in clinical microbiology laboratories for diagnosis of sepsis. Objectives: To compare the conventional blood culture method with the lysis centrifugation method in cases of sepsis. Materials and Methods: Two hundred ...

Implementation of centrifuge testing of expansive soils for pavement design.

Science.gov (United States)

2017-03-01

The novel centrifuge-based method for testing of expansive soils from project 5-6048-01 was implemented into : use for the determination of the Potential Vertical Rise (PVR) of roadways that sit on expansive subgrades. The : centrifuge method was mod...

75 FR 70300 - USEC, Inc.; American Centrifuge Lead Cascade Facility; American Centrifuge Plant; Notice of...

Science.gov (United States)

2010-11-17

... Centrifuge Lead Cascade Facility; American Centrifuge Plant; Notice of Receipt of a License Transfer... SNM-2011, for the American Centrifuge Lead Cascade Facility and the American Centrifuge Plant... USEC Inc., (the Licensee), for its American Centrifuge Lead Cascade Facility (LCF) and American...

Study on the Performance of a Centrifugal Compressor Using Fluid-Structure Interaction Method

Energy Technology Data Exchange (ETDEWEB)

Lee Horim; Hwang, Yoonjei; Jeong, Jinhee [LG Electronics, Seoul (Korea, Republic of); Kim, Changhee; Yang, Jangsik; Son, Changmin [Pusan Nat’l Univ., Busan (Korea, Republic of)

2016-06-15

In this study, we perform a series of aero-thermo-mechanical analyses to predict the running-tip clearance and the effects of impeller deformation on the performance using a centrifugal compressor. During operation, the impeller deformation due to a combination of the centrifugal force, aerodynamic pressure and the thermal load results in a non-uniform tip clearance profile. For the prediction, we employ the one-way fluid-structure interaction (FSI) method using CFX 14.5 and ANSYS. The predicted running tip clearance shows a non-uniform profile over the entire flow passage. In particular, a significant reduction of the tip clearance height occurred at the leading and trailing edges of the impeller. Because of the reduction of the tip clearance, the tip leakage flow decreased by 19.4% In addition, the polytrophic efficiency under operating conditions increased by 0.72%. These findings confirm that the prediction of the running tip clearance and its impact on compressor performance is an important area that requires further investigation.

Study on the Performance of a Centrifugal Compressor Using Fluid-Structure Interaction Method

International Nuclear Information System (INIS)

Lee Horim; Hwang, Yoonjei; Jeong, Jinhee; Kim, Changhee; Yang, Jangsik; Son, Changmin

2016-01-01

In this study, we perform a series of aero-thermo-mechanical analyses to predict the running-tip clearance and the effects of impeller deformation on the performance using a centrifugal compressor. During operation, the impeller deformation due to a combination of the centrifugal force, aerodynamic pressure and the thermal load results in a non-uniform tip clearance profile. For the prediction, we employ the one-way fluid-structure interaction (FSI) method using CFX 14.5 and ANSYS. The predicted running tip clearance shows a non-uniform profile over the entire flow passage. In particular, a significant reduction of the tip clearance height occurred at the leading and trailing edges of the impeller. Because of the reduction of the tip clearance, the tip leakage flow decreased by 19.4% In addition, the polytrophic efficiency under operating conditions increased by 0.72%. These findings confirm that the prediction of the running tip clearance and its impact on compressor performance is an important area that requires further investigation.

Thermally-controlled centrifuge for isotopic separation

International Nuclear Information System (INIS)

Cenedese, A.; Cunsolo, D.

1976-01-01

Among the various methods proposed to obtain lighter component enrichment in the isotopic separation of uranium, ultracentrifugation is becoming more and more interesting today, as this process becomes a useful alternate method to gaseous diffusion. The ultracentrifuge main gas-dynamic features are investigated in the present study. In particular, the field inside the centrifuge has been subdivided into three axial zones: an internal central zone, characterized by an essentially axial flow; two external zones, near the two caps of the centrifuge; two intermediate zones, of a length of the order of the radius. For the analytical solution the linearized Navier-Stokes equations have been considered. The central zone flow is solved by separating the independent variables; the corresponding eigenvalue problem has been solved numerically. A series of eigensolutions which satisfy boundary conditions at the walls of the cylinder has been calculated. An integral method for the superimposition of the above mentioned eigensolutions is proposed in order to satisfy the conditions at the tops for thermally-controlled centrifuges. (author)

A comparison of two centrifuge techniques for constructing vulnerability curves: insight into the 'open-vessel' artifact.

Science.gov (United States)

Yin, Pengxian; Meng, Feng; Liu, Qing; An, Rui; Cai, Jing; Du, Guangyuan

2018-03-30

A vulnerability curve (VC) describes the extent of xylem cavitation resistance. Centrifuges have been used to generate VCs for decades via static- and flow-centrifuge methods. Recently, the validity of the centrifuge techniques has been questioned. Researchers have hypothesized that the centrifuge techniques might yield unreliable VCs due to the open-vessel artifact. However, other researchers reject this hypothesis. The focus of the dispute is centred on whether exponential VCs are more reliable when the static-centrifuge method is used than with the flow-centrifuge method. To further test the reliability of the centrifuge technique, two centrifuges were manufactured to simulate the static- and flow-centrifuge methods. VCs of three species with open vessels of known lengths were constructed using the two centrifuges. The results showed that both centrifuge techniques produced invalid VCs for Robinia because the water flow through stems under mild tension in centrifuges led to an increasing loss of water conductivity. Additionally, the injection of water in the flow-centrifuge exacerbated the loss of water conductivity. However, both centrifuge techniques yielded reliable VCs for Prunus, regardless of the presence of open vessels in the tested samples. We conclude that centrifuge techniques can be used in species with open vessels only when the centrifuge produces a VC that matches the bench-dehydration VC. This article is protected by copyright. All rights reserved.

Modelling of hydrothermal characteristics of centrifugal nozzles

International Nuclear Information System (INIS)

Yarkho, A.A.; Omelchenko, M.P.; Borshchev, V.A.

1990-01-01

Presented for the first time is a method of recalculating the hydrothermal characteristics of centrifugal nozzles obtained in laboratory conditions for full-scale nozzles. From the experimental hydrothermal characteristics of nozzles observed in the laboratory it is allowed to calculate the hydrothermal characteristics of any other centrifugal nozzle whose diameter and dimensionless geometric characteristic are known

CENTRIFUGE

Science.gov (United States)

Rushing, F.C.

1960-09-01

A vibration damping mechanism for damping vibration forces occurring during the operation of a centrifuge is described. The vibration damping mechanism comprises a plurality of nested spaced cylindrical elements surrounding the rotating shaft of the centrifuge. Some of the elements are held substantially stationary while the others are held with respect to a pair of hearings spaced along the rotating shaft. A fluid is retained about the cylindrical elements.

Modeling centrifugal cell washers using computational fluid dynamics.

Science.gov (United States)

Kellet, Beth E; Han, Binbing; Dandy, David S; Wickramasinghe, S Ranil

2004-11-01

Reinfusion of shed blood during surgery could avoid the need for blood transfusions. Prior to reinfusion of the red blood cells, the shed blood must be washed in order to remove leukocytes, platelets, and other contaminants. Further, the hematocrit of the washed blood must be increased. The feasibility of using computational fluid dynamics (CFD) to guide the design of better centrifuges for processing shed blood is explored here. The velocity field within a centrifuge bowl and the rate of protein removal from the shed blood has been studied. The results obtained indicate that CFD could help screen preliminary centrifuge bowl designs, thus reducing the number of initial experimental tests required when developing new centrifuge bowls. Although the focus of this work is on washing shed blood, the methods developed here are applicable to the design of centrifuge bowls for other blood-processing applications.

On compressible flow in a gas centrifuge and its effect on the maximum separative power

NARCIS (Netherlands)

Brouwers, J.J.H.

1978-01-01

The gas circulation in a gas centrifuge due to temperature differences, differential rotation and injection, and removal of fluid at the ends, as well as due to temperature gradients at the cylinder wall, is treated analytically. The motion consists of a small perturbation on a state of isothermal

RESEARCH CENTRIFUGE- ADVANCED TOOL SEPERATION

OpenAIRE

Mahajan Ashwini; Prof. B.V. Jain; Dr Surajj Sarode

2015-01-01

A centrifuge is a critical piece of equipment for the laboratory. Purpose of this study was to study research centrifuge in detail, its applications, uses in different branches and silent features. Their are two types of research centrifuge study here revolutionary research centrifuge and microprocessor research centrifuge. A centrifuge is a device that separates particles from a solution through use of a rotor. In biology, the particles are usually cells, sub cellular organelles, or large mo...

Pipeline system for gas centrifuge

International Nuclear Information System (INIS)

Masumoto, Tsutomu; Umezawa, Sadao.

1977-01-01

Purpose: To enable effective operation for the gas centrifuge cascade system upon failures in the system not by interrupting the operation of all of the centrifuges in the system but by excluding only the failed centrifuges. Constitution: A plurality of gas centrifuges are connected by way of a pipeline and an abnormal detector for the automatic detection of abnormality such as destruction in a vacuum barrel and loss of vacuum is provided to each of the centrifuges. Bypass lines for short-circuitting adjacent centrifuges are provided in the pipelines connecting the centrifuges. Upon generation of abnormality in a centrifuge, a valve disposed in the corresponding bypass is automatically closed or opened by a signal from the abnormal detector to change the gas flow to thereby exclude the centrifuge in abnormality out of the system. This enables to effectively operate the system without interrupting the operation for the entire system. (Moriyama, K.)

PARAMETRIC DIAGNOSTICS OF THE CENTRIFUGAL SUPERCHARGER'S TECHNICAL CONDITION DURING OPERATION

Directory of Open Access Journals (Sweden)

Regina A. Khuramshina

2017-01-01

Full Text Available Abstract. Objectives The main aim is to develop a mathematical model of a centrifugal compressor and carry out a parametric diagnostics of a centrifugal supercharger's technical condition during operation. Methods  A model is proposed for calculating the thermodynamic properties of natural gas, reducing the parameters of a centrifugal compressor to the initial conditions and to the rotation frequency, as well as the integral indicators of the supercharger's technical state. The technical state of the gas path of the centrifugal supercharger of the compressor unit is determined by the parametric diagnostic method. Results  The software implementation of the mathematical model of centrifugal compressor is carried out using a DVIGwT PC. The analysis of calculations indicates that the model is appropriate, with the error being due to taking into account the properties of iso-butane and i-hexane, in contrast with the VNIIGAZ technique. The evaluation studies of a centrifugal compressor's state are indicative of the presence or absence of its defects. Conclusion  Among a number of the diagnostic methods for evaluating a centrifugal supercharger, the most effective is vibrodiagnostics. However, the search for malfunctions and nascent defects in the flowing part of the centrifugal compressor cannot be limited only to vibrodiagnostic data, which provides about 60% of the reliable information about the state of the gas-air tract. About 20% of the compressor's malfunctions and approximately half of the dangerous modes of the supercharger's flow-through part is detected using thermogasdynamic parametric analysis (parametric diagnostics. The main difficulty of the control over the technical state of the flow-through part of the centrifugal supercharger is in the complication of the quantitative evaluation of the processes taking place in the supercharger, which leads to problems in providing reliable diagnosis during a reasonable period of time.

Plasma centrifuges

International Nuclear Information System (INIS)

Karchevskij, A.I.; Potanin, E.P.

2000-01-01

The review of the most important studies on the isotope separation processes in the rotating plasma is presented. The device is described and the characteristics of operation of the pulse plasma centrifuges with weakly and strongly ionized plasma as well as the stationary plasma centrifuges with the medium weak ionization and devices, applying the stationary vacuum arc with the high ionization rate and the stationary beam-plasma discharge with complete ionization, are presented. The possible mechanisms of the isotope separation in plasma centrifuges are considered. The specific energy consumption for isotope separation in these devices is discussed [ru

Gradient-based methods for production optimization of oil reservoirs

Energy Technology Data Exchange (ETDEWEB)

Suwartadi, Eka

2012-07-01

Production optimization for water flooding in the secondary phase of oil recovery is the main topic in this thesis. The emphasis has been on numerical optimization algorithms, tested on case examples using simple hypothetical oil reservoirs. Gradientbased optimization, which utilizes adjoint-based gradient computation, is used to solve the optimization problems. The first contribution of this thesis is to address output constraint problems. These kinds of constraints are natural in production optimization. Limiting total water production and water cut at producer wells are examples of such constraints. To maintain the feasibility of an optimization solution, a Lagrangian barrier method is proposed to handle the output constraints. This method incorporates the output constraints into the objective function, thus avoiding additional computations for the constraints gradient (Jacobian) which may be detrimental to the efficiency of the adjoint method. The second contribution is the study of the use of second-order adjoint-gradient information for production optimization. In order to speedup convergence rate in the optimization, one usually uses quasi-Newton approaches such as BFGS and SR1 methods. These methods compute an approximation of the inverse of the Hessian matrix given the first-order gradient from the adjoint method. The methods may not give significant speedup if the Hessian is ill-conditioned. We have developed and implemented the Hessian matrix computation using the adjoint method. Due to high computational cost of the Newton method itself, we instead compute the Hessian-timesvector product which is used in a conjugate gradient algorithm. Finally, the last contribution of this thesis is on surrogate optimization for water flooding in the presence of the output constraints. Two kinds of model order reduction techniques are applied to build surrogate models. These are proper orthogonal decomposition (POD) and the discrete empirical interpolation method (DEIM

Several Guaranteed Descent Conjugate Gradient Methods for Unconstrained Optimization

Directory of Open Access Journals (Sweden)

San-Yang Liu

2014-01-01

Full Text Available This paper investigates a general form of guaranteed descent conjugate gradient methods which satisfies the descent condition gkTdk≤-1-1/4θkgk2  θk>1/4 and which is strongly convergent whenever the weak Wolfe line search is fulfilled. Moreover, we present several specific guaranteed descent conjugate gradient methods and give their numerical results for large-scale unconstrained optimization.

Improved quality of cryopreserved cheetah (Acinonyx jubatus) spermatozoa after centrifugation through Accudenz.

Science.gov (United States)

Crosier, Adrienne E; Henghali, Josephine N; Howard, Jogayle; Pukazhenthi, Budhan S; Terrell, Kimberly A; Marker, Laurie L; Wildt, David E

2009-01-01

Sperm cryopreservation, in combination with assisted reproductive techniques, is a valuable tool for the genetic management of endangered felids. However, the acrosome of the cheetah spermatozoon is especially sensitive to cryopreservation, with approximately 40% of spermatozoa experiencing acrosomal damage immediately after thawing and then another approximately 15% loss during the next 4 hours in vitro. Additionally, thawing causes a reduction in sperm motility by approximately 20% with another decrease of approximately 12% during subsequent incubation in vitro. We hypothesized that slow removal of glycerol from cryopreserved cheetah spermatozoa using an Accudenz gradient would improve acrosomal integrity, sperm motility longevity, and structural morphology. Accudenz was compared with traditional cheetah sperm processing methods for glycerol removal that involves washing, multistep resuspension, and swim-up processing. Electroejaculates (n = 21 total from 8 males) were washed in Ham F10 medium, and sperm pellets were resuspended in TEST-yolk buffer with 0% glycerol. Samples were cryopreserved in straws in 4% final glycerol, thawed, and assessed for percent intact acrosomes (% IA), percent motility (% M), and forward progressive status (FPS; scale, 0-5). Sperm motility index (SMI) was calculated as (% M + [FPS x 20]) / 2. In study 1, glycerol removal by centrifugation through an Accudenz gradient (4%, 10%) was compared with traditional sperm washing (control) and multistep resuspension protocols. At each time after centrifugation (hourly for 4 hours), % IA was improved (P cheetah sperm mitigates the significant loss in sperm quality that occurs after freeze-thawing. This alleviation of cellular damage resulting from cryopreservation contributes to a more than 10% improvement in overall sperm motility and, more importantly, allows retention of 40% or more of sperm with intact acrosomes.

Isolation of Mycobacterium chelonei with the lysis-centrifugation blood culture technique.

OpenAIRE

Fojtasek, M F; Kelly, M T

1982-01-01

Mycobacterium chelonei was isolated from a patient by the lysis-centrifugation and the conventional two-bottle blood culture methods. The lysis-centrifugation method was significantly more sensitive and rapid than the conventional method in detecting and isolating this organism; quantitations done by this method were useful for monitoring response to therapy.

M-step preconditioned conjugate gradient methods

Science.gov (United States)

Adams, L.

1983-01-01

Preconditioned conjugate gradient methods for solving sparse symmetric and positive finite systems of linear equations are described. Necessary and sufficient conditions are given for when these preconditioners can be used and an analysis of their effectiveness is given. Efficient computer implementations of these methods are discussed and results on the CYBER 203 and the Finite Element Machine under construction at NASA Langley Research Center are included.

Simulation of a Centrifugal Pump by Using the Harmonic Balance Method

Directory of Open Access Journals (Sweden)

Franco Magagnato

2015-01-01

Full Text Available The harmonic balance method was used for the flow simulation in a centrifugal pump. Independence studies have been done to choose proper number of harmonic modes and inlet eddy viscosity ratio value. The results from harmonic balance method show good agreements with PIV experiments and unsteady calculation results (which is based on the dual time stepping method for the predicted head and the phase-averaged velocity. A detailed analysis of the flow fields at different flow rates shows that the flow rate has an evident influence on the flow fields. At 0.6Qd, some vortices begin to appear in the impeller, and at 0.4Qd some vortices have blocked the flow passage. The flow fields at different positions at 0.6Qd and 0.4Qd show how the complicated flow phenomena are forming, developing, and even disappearing. The harmonic balance method can be used for the flow simulation in pumps, showing the same accuracy as unsteady methods, but is considerably faster.

The effect of centrifugation at various g force levels on rheological properties of rat, dog, pig and human red blood cells.

Science.gov (United States)

Kiss, Ferenc; Toth, Eniko; Miszti-Blasius, Kornel; Nemeth, Norbert

2016-01-01

Laboratory investigations often require centrifugation of blood samples for various erythrocyte tests. Although there is a lack of data about the effect of centrifugation at various g force levels on erythrocyte rheological properties. We aimed to investigate the effect of a 10-minute centrifugation at 500, 1000 or 1500 g at 15°C of rat, dog, pig and human venous (K3-EDTA, 1.5 mg/ml) blood samples. Hematological parameters, erythrocyte deformability, cell membrane stability, osmotic gradient ektacytometry (osmoscan) and erythrocyte aggregation were determined. Hematological and erythrocyte deformability parameters showed interspecies differences, centrifugation caused no significant alterations. Cell membrane stability for human erythrocytes centrifuged at higher g level showed less decrease in deformability. Osmoscan O min parameter showed slight elevation in dog centrifuged aliquots. Erythrocyte aggregation parameters changed unexpectedly. Rat and dog erythrocyte aggregation indices significantly dropped in centrifuged aliquots. Pig erythrocyte aggregation indices increased significantly after centrifugation. Human erythrocyte aggregation was the most stable one among the investigated species. The used centrifugation protocols caused the largest alterations in erythrocyte aggregation in a controversial way among the investigated species. On the other hand, erythrocyte deformability parameters were stable, cell membrane stability and osmoscan data show minor shifts.

Numerical simulation of the unsteady progress in centrifuge

International Nuclear Information System (INIS)

Wei Chunlin; Zeng Shi

2006-01-01

Unsteady flow equations for the centrifuge are solved on a staggered grid by a finite volume method. The transient process that the axial flow in the centrifuge is established under a steady thermal driving. It can be concluded that the influence which causes the perturbing fluid is different at the beginning and the end of the processing. The flow is caused by the imbalance of temperature which turns to be caused by the imbalance of pressure. The results show that the numerical simulation is effective at the unsteady fluid in a centrifuge. (authors)

76 FR 9613 - USEC Inc. (American Centrifuge Lead Cascade Facility and American Centrifuge Plant); Order...

Science.gov (United States)

2011-02-18

... NUCLEAR REGULATORY COMMISSION [EA-11-013] USEC Inc. (American Centrifuge Lead Cascade Facility and American Centrifuge Plant); Order Approving Direct Transfer of Licenses and Conforming Amendment I USEC... Centrifuge Lead Cascade Facility (Lead Cascade) and American Centrifuge Plant (ACP), respectively, which...

77 FR 9273 - USEC Inc. (American Centrifuge Lead Cascade Facility and American Centrifuge Plant); Direct...

Science.gov (United States)

2012-02-16

... NUCLEAR REGULATORY COMMISSION [NRC-2010-0355] USEC Inc. (American Centrifuge Lead Cascade Facility and American Centrifuge Plant); Direct Transfer of Licenses In the Matter of USEC INC. (American Centrifuge Lead Cascade Facility and American Centrifuge Plant); Order EA-12- [[Page 9274

Accelerated gradient methods for total-variation-based CT image reconstruction

Energy Technology Data Exchange (ETDEWEB)

Joergensen, Jakob H.; Hansen, Per Christian [Technical Univ. of Denmark, Lyngby (Denmark). Dept. of Informatics and Mathematical Modeling; Jensen, Tobias L.; Jensen, Soeren H. [Aalborg Univ. (Denmark). Dept. of Electronic Systems; Sidky, Emil Y.; Pan, Xiaochuan [Chicago Univ., Chicago, IL (United States). Dept. of Radiology

2011-07-01

Total-variation (TV)-based CT image reconstruction has shown experimentally to be capable of producing accurate reconstructions from sparse-view data. In particular TV-based reconstruction is well suited for images with piecewise nearly constant regions. Computationally, however, TV-based reconstruction is demanding, especially for 3D imaging, and the reconstruction from clinical data sets is far from being close to real-time. This is undesirable from a clinical perspective, and thus there is an incentive to accelerate the solution of the underlying optimization problem. The TV reconstruction can in principle be found by any optimization method, but in practice the large scale of the systems arising in CT image reconstruction preclude the use of memory-intensive methods such as Newton's method. The simple gradient method has much lower memory requirements, but exhibits prohibitively slow convergence. In the present work we address the question of how to reduce the number of gradient method iterations needed to achieve a high-accuracy TV reconstruction. We consider the use of two accelerated gradient-based methods, GPBB and UPN, to solve the 3D-TV minimization problem in CT image reconstruction. The former incorporates several heuristics from the optimization literature such as Barzilai-Borwein (BB) step size selection and nonmonotone line search. The latter uses a cleverly chosen sequence of auxiliary points to achieve a better convergence rate. The methods are memory efficient and equipped with a stopping criterion to ensure that the TV reconstruction has indeed been found. An implementation of the methods (in C with interface to Matlab) is available for download from http://www2.imm.dtu.dk/~pch/TVReg/. We compare the proposed methods with the standard gradient method, applied to a 3D test problem with synthetic few-view data. We find experimentally that for realistic parameters the proposed methods significantly outperform the standard gradient method. (orig.)

Theoretical prediction and validation of cell recovery rates in preparing platelet-rich plasma through a centrifugation.

Science.gov (United States)

Piao, Linfeng; Park, Hyungmin; Jo, Chris Hyunchul

2017-01-01

In the present study, we propose a theoretical framework to predict the recovery rates of platelets and white blood cells in the process of centrifugal separation of whole blood contained in a tube for the preparation of platelet-rich plasma. Compared to previous efforts to optimize or standardize the protocols of centrifugation, we try to further the physical background (i.e., based on the multiphase flow phenomena) of analysis to develop a universal approach that can be applied to widely different conditions. That is, one-dimensional quasi-linear partial differential equation to describe the centrifugal sedimentation of dispersed phase (red and white blood cells) in continuous phase (plasma) is derived based on the kinematic-wave theory. With the information of whole blood volume and tube geometry considered, it is possible to determine the positions of interfaces between supernatant/suspension and suspension/sediment, i.e., the particle concentration gradient in a tube, for a wide range of centrifugation parameters (time and acceleration). While establishing a theory to predict the recovery rates of the platelet and white blood cell from the pre-determined interface positions, we also propose a new correlation model between the recovery rates of plasma and platelets, which is found to be a function of the whole blood volume, centrifugal time and acceleration, and tube geometry. The present predictions for optimal condition show good agreements with available human clinical data, obtained from different conditions, indicating the universal applicability of our method. Furthermore, the dependence of recovery rates on centrifugal conditions reveals that there exist a different critical acceleration and time for the maximum recovery rate of platelets and white blood cells, respectively. The other parameters such as hematocrit, whole blood volume and tube geometry are also found to strongly affect the maximum recovery rates of blood cells, and finally, as a strategy

Analytical Energy Gradients for Excited-State Coupled-Cluster Methods

Science.gov (United States)

Wladyslawski, Mark; Nooijen, Marcel

The equation-of-motion coupled-cluster (EOM-CC) and similarity transformed equation-of-motion coupled-cluster (STEOM-CC) methods have been firmly established as accurate and routinely applicable extensions of single-reference coupled-cluster theory to describe electronically excited states. An overview of these methods is provided, with emphasis on the many-body similarity transform concept that is the key to a rationalization of their accuracy. The main topic of the paper is the derivation of analytical energy gradients for such non-variational electronic structure approaches, with an ultimate focus on obtaining their detailed algebraic working equations. A general theoretical framework using Lagrange's method of undetermined multipliers is presented, and the method is applied to formulate the EOM-CC and STEOM-CC gradients in abstract operator terms, following the previous work in [P.G. Szalay, Int. J. Quantum Chem. 55 (1995) 151] and [S.R. Gwaltney, R.J. Bartlett, M. Nooijen, J. Chem. Phys. 111 (1999) 58]. Moreover, the systematics of the Lagrange multiplier approach is suitable for automation by computer, enabling the derivation of the detailed derivative equations through a standardized and direct procedure. To this end, we have developed the SMART (Symbolic Manipulation and Regrouping of Tensors) package of automated symbolic algebra routines, written in the Mathematica programming language. The SMART toolkit provides the means to expand, differentiate, and simplify equations by manipulation of the detailed algebraic tensor expressions directly. The Lagrangian multiplier formulation establishes a uniform strategy to perform the automated derivation in a standardized manner: A Lagrange multiplier functional is constructed from the explicit algebraic equations that define the energy in the electronic method; the energy functional is then made fully variational with respect to all of its parameters, and the symbolic differentiations directly yield the explicit

Thermal hydrodynamic analysis of a countercurrent gas centrifuge

International Nuclear Information System (INIS)

Andrade, Delvonei Alves de

1999-01-01

The influence of the thermal countercurrent on the separative performance of countercurrent centrifuges is treated in this work. The methodology used consists in modeling the gas flow inside the rotor under thermal boundary conditions supplied by the structural thermal model. The gas flow model, also called hydrodynamical model, is based on the Finite Volume Method for cylindrical geometry with azimuthal symmetry. The structural thermal model is based on the Nodal Method and take into account simultaneously, the conduction convection and radiation phenomena. The procedure adopted for this study consisted in the definition of the operational and geometric conditions of a centrifuge which was used as a pattern to the accomplished analysis. This configuration, called 'Standard Centrifuge', was used for the accomplishment of several simulations where the importance of the realistic boundary thermal conditions for the numerical evaluation of the centrifuge separative capacity was evidenced. A selective alteration for the optical properties based on simple engineering procedures was proposed. An improvement of 5% was obtained with this alteration. (author)

Gradient porous hydroxyapatite ceramics fabricated by freeze casting method

International Nuclear Information System (INIS)

Zuo Kaihui; Zhang Yuan; Jiang Dongliang; Zeng Yuping

2011-01-01

By controlling the cooling rates and the composition of slurries, the gradient porous hydroxyapatite ceramics are fabricated by the freeze casting method. According to the different cooling rate, the pores of HAP ceramics fabricated by gradient freeze casting are divided into three parts: one is lamellar pores, another is column pore and the last one is fine round pores. The laminated freeze casting is in favour of obtaining the gradient porous ceramics composed of different materials and the ceramics have unclear interfaces.

Sand characterization by combined centrifuge and laboratory tests

OpenAIRE

GAUDIN, C; SCHNAID, F; GARNIER, J

2005-01-01

The purpose of this paper is to evaluate new methods of interpretation of in situ tests in sand from correlations established from centrifuge and laboratory data. Emphasis is given to methods that are based on the combination of measurements from independent tests, such as the ratio of the elastic stiffness to ultimate strenght and the ratio of cone resistance and limit pressure. For that purpose, a series of centrifuge tests using a cone penetrometer and a cone pressuremeter was carried out ...

Comparison of PCR-Based Diagnosis with Centrifuged-Based Enrichment Method for Detection of Borrelia persica in Animal Blood Samples.

Science.gov (United States)

Naddaf, S R; Kishdehi, M; Siavashi, Mr

2011-01-01

The mainstay of diagnosis of relapsing fever (RF) is demonstration of the spirochetes in Giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by microscopy. This study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spirochetemia. Animal blood samples with low degrees of spirochetemia were tested with two PCRs and a nested PCR targeting flaB, GlpQ, and rrs genes. Also, a centrifuged-based enrichment method and Giemsa staining were performed on blood samples with various degrees of spirochetemia. The flaB-PCR and nested rrs-PCR turned positive with various degrees of spirochetemia including the blood samples that turned negative with dark-field microscopy. The GlpQ-PCR was positive as far as at least one spirochete was seen in 5-10 microscopic fields. The sensitivity of GlpQ-PCR increased when DNA from Buffy Coat Layer (BCL) was used as template. The centrifuged-based enrichment method turned positive with as low concentration as 50 bacteria/ml blood, while Giemsa thick staining detected bacteria with concentrations ≥ 25000 bacteria/ml. Centrifuged-based enrichment method appeared as much as 500-fold more sensitive than thick smears, which makes it even superior to some PCR assays. Due to simplicity and minimal laboratory requirements, this method can be considered a valuable tool for diagnosis of RF in rural health centers.

Developing an industrial centrifugal fan as prototype using an experiment series and finite volume method

OpenAIRE

GÜRSEL, Onur; ERKEK, Mehmet

2012-01-01

Centrifugal fans are used in most of the manufacturing processes for ventilating and/or air conditioning the manufacturing areas. Because of relatively limited documentation on design of these fans, new designs are developed by experimental method. This method does not only take a lot of time but also increases the costs considerably. Nowadays, most of the companies create 3D models and then conduct analyses by the CFD (computational fluid dynamics) programs and perform some optimizations bef...

Centrifugal pump handbook

CERN Document Server

Pumps, Sulzer

2010-01-01

This long-awaited new edition is the complete reference for engineers and designers working on pump design and development or using centrifugal pumps in the field. This authoritative guide has been developed with access to the technical expertise of the leading centrifugal pump developer, Sulzer Pumps. In addition to providing the most comprehensive centrifugal pump theory and design reference with detailed material on cavitation, erosion, selection of materials, rotor vibration behavior and forces acting on pumps, the handbook also covers key pumping applications topics and operational

A gradient-based method for segmenting FDG-PET images: methodology and validation

International Nuclear Information System (INIS)

Geets, Xavier; Lee, John A.; Gregoire, Vincent; Bol, Anne; Lonneux, Max

2007-01-01

A new gradient-based method for segmenting FDG-PET images is described and validated. The proposed method relies on the watershed transform and hierarchical cluster analysis. To allow a better estimation of the gradient intensity, iteratively reconstructed images were first denoised and deblurred with an edge-preserving filter and a constrained iterative deconvolution algorithm. Validation was first performed on computer-generated 3D phantoms containing spheres, then on a real cylindrical Lucite phantom containing spheres of different volumes ranging from 2.1 to 92.9 ml. Moreover, laryngeal tumours from seven patients were segmented on PET images acquired before laryngectomy by the gradient-based method and the thresholding method based on the source-to-background ratio developed by Daisne (Radiother Oncol 2003;69:247-50). For the spheres, the calculated volumes and radii were compared with the known values; for laryngeal tumours, the volumes were compared with the macroscopic specimens. Volume mismatches were also analysed. On computer-generated phantoms, the deconvolution algorithm decreased the mis-estimate of volumes and radii. For the Lucite phantom, the gradient-based method led to a slight underestimation of sphere volumes (by 10-20%), corresponding to negligible radius differences (0.5-1.1 mm); for laryngeal tumours, the segmented volumes by the gradient-based method agreed with those delineated on the macroscopic specimens, whereas the threshold-based method overestimated the true volume by 68% (p = 0.014). Lastly, macroscopic laryngeal specimens were totally encompassed by neither the threshold-based nor the gradient-based volumes. The gradient-based segmentation method applied on denoised and deblurred images proved to be more accurate than the source-to-background ratio method. (orig.)

A Spectral Conjugate Gradient Method for Unconstrained Optimization

International Nuclear Information System (INIS)

Birgin, E. G.; Martinez, J. M.

2001-01-01

A family of scaled conjugate gradient algorithms for large-scale unconstrained minimization is defined. The Perry, the Polak-Ribiere and the Fletcher-Reeves formulae are compared using a spectral scaling derived from Raydan's spectral gradient optimization method. The best combination of formula, scaling and initial choice of step-length is compared against well known algorithms using a classical set of problems. An additional comparison involving an ill-conditioned estimation problem in Optics is presented

Application of preconditioned conjugate gradient-like methods to reactor kinetics

International Nuclear Information System (INIS)

Yang, D.Y.; Chen, G.S.; Chou, H.P.

1993-01-01

Several conjugate gradient-like (CG-like) methods are applied to solve the nonsymmetric linear systems of equations derived from the time-dependent two-dimensional two-energy-group neutron diffusion equations by a finite difference approximation. The methods are: the generalized conjugate residual method; the generalized conjugate gradient least-square method; the generalized minimal residual method (GMRES); the conjugate gradient square method; and a variant of bi-conjugate gradient method (Bi-CGSTAB). In order to accelerate these methods, six preconditioning techniques are investigated. Two are based on pointwise incomplete factorization: the incomplete LU (ILU) and the modified incomplete LU (MILU) decompositions; two, based on the block tridiagonal structure of the coefficient matrix, are blockwise and modified blockwise incomplete factorizations, BILU and MBILU; two are the alternating-direction implicit and symmetric successive overrelaxation (SSOR) preconditioners, derived from the basic iterative schemes. Comparisons are made by using CG-like methods combined with different preconditioners to solve a sequence of time-step reactor transient problems. Numerical tests indicate that preconditioned BI-CGSTAB with the preconditioner MBILU requires less CPU time and fewer iterations than other methods. The preconditioned CG-like methods are less sensitive to the time-step size used than the Chebyshev semi-iteration method and line SOR method. The indication is that the CGS, Bi-CGSTAB and GMRES methods are, on average, better than the other methods in reactor kinetics computation and that a good preconditioner is more important than the choice of CG-like methods. The MILU decomposition based on the conventional row-sum criterion has difficulty yielding a convergent solution and an improved version is introduced. (author)

A feasible DY conjugate gradient method for linear equality constraints

Science.gov (United States)

LI, Can

2017-09-01

In this paper, we propose a feasible conjugate gradient method for solving linear equality constrained optimization problem. The method is an extension of the Dai-Yuan conjugate gradient method proposed by Dai and Yuan to linear equality constrained optimization problem. It can be applied to solve large linear equality constrained problem due to lower storage requirement. An attractive property of the method is that the generated direction is always feasible and descent direction. Under mild conditions, the global convergence of the proposed method with exact line search is established. Numerical experiments are also given which show the efficiency of the method.

Centrifugation: an important pre-analytic procedure that influences plasma microRNA quantification during blood processing.

Science.gov (United States)

Zheng, Xiao-Hui; Cui, Cui; Zhou, Xin-Xi; Zeng, Yi-Xin; Jia, Wei-Hua

2013-12-01

Circulating microRNAs are robustly present in plasma or serum and have become a research focus as biomarkers for tumor diagnosis and prognosis. Centrifugation is a necessary procedure for obtaining high-quality blood supernatant. Herein, we investigated one-step and two-step centrifugations, two centrifugal methods routinely used in microRNA study, to explore their effects on plasma microRNA quantification. The microRNAs obtained from one-step and two-step centrifugations were quantified by microarray and TaqMan-based real-time quantitative polymerase chain reaction (Q-PCR). Dynamic light scattering was performed to explore the difference underlying the two centrifugal methods. The results from the microarray containing 1,347 microRNAs showed that the signal detection rate was greatly decreased in the plasma sample prepared by two-step centrifugation. More importantly, the microRNAs missing in this plasma sample could be recovered and detected in the precipitate generated from the second centrifugation. Consistent with the results from microarray, a marked decrease of three representative microRNAs in two-step centrifugal plasma was validated by Q-PCR. According to the size distribution of all nanoparticles in plasma, there were fewer nanoparticles with size >1,000 nm in two-step centrifugal plasma. Our experiments directly demonstrated that different centrifugation methods produced distinct quantities of plasma microRNAs. Thus, exosomes or protein complexes containing microRNAs may be involved in large nanoparticle formation and may be precipitated after two-step centrifugation. Our results remind us that sample processing methods should be first considered in conducting research.

Quantification of platelets obtained by different centrifugation protocols in SHR rats.

Science.gov (United States)

Yazigi Junior, João Alberto; Dos Santos, João Baptista Gomes; Xavier, Bruno Rodrigues; Fernandes, Marcela; Valente, Sandra Gomes; Leite, Vilnei Mattiolli

2015-01-01

To quantify the platelet concentration in the blood of SHR rats, by means of different centrifugation protocols, and to evaluate what the most effective method for obtaining platelets is. We used 40 male rats of the isogenic SHR lineage. The animals were divided into three groups: control, using whole blood without centrifugation; single centrifugation, using whole blood subjected to a single centrifugation at 200 × g and 400 × g; and double centrifugation, using whole blood subjected one centrifugation at different rotations, followed by collection of whole plasma subjected to another centrifugation at different rotations: 200 × g + 200 × g; 200 × g + 400 × g; 200 × g + 800 × g; 400 × g + 400 × g; 400 × g + 800 × g. Samples of 3 ml of blood were drawn from each animal by means of cardiac puncture. The blood was stored in Vacutainer collection tubes containing 3.2% sodium citrate. The blood from the control group animals was analyzed without being subjected to centrifugation. After the blood from the other groups of animals had been subjected to centrifugation, the whole plasma was collected and subjected to platelet counting in the lower third of the sample. We obtained greatest platelet enrichment in the subgroup with two centrifugations comprising 400 × g for 10 min + 400 × g for 10 min, in which the mean platelet concentration was 11.30 times higher than that of the control group. It was possible to obtain a high platelet concentration using viable simple techniques, by means of centrifugation of whole blood and use of commonly used materials. The most effective method for obtaining platelet concentrate was found in samples subjected to two centrifugations.

Enrichment: centrifuge process

International Nuclear Information System (INIS)

Soubbaramayer.

1989-01-01

This short course is divided into three sections devoted respectively to the physics of the process, some practical problems raised by the design of a centrifuge and the present situation of centrifugation in the World. 31 figs., 18 refs

Model Reference Adaptive Control of the Air Flow Rate of Centrifugal Compressor Using State Space Method

International Nuclear Information System (INIS)

Han, Jaeyoung; Jung, Mooncheong; Yu, Sangseok; Yi, Sun

2016-01-01

In this study, a model reference adaptive controller is developed to regulate the outlet air flow rate of centrifugal compressor for automotive supercharger. The centrifugal compressor is developed using the analytical based method to predict the transient behavior of operating and the designed model is validated with experimental data to confirm the system accuracy. The model reference adaptive control structure consists of a compressor model and a MRAC(model reference adaptive control) mechanism. The feedback control do not robust with variation of system parameter but the applied adaptive control is robust even if the system parameter is changed. As a result, the MRAC was regulated to reference air flow rate. Also MRAC was found to be more robust control compared with the feedback control even if the system parameter is changed.

Model Reference Adaptive Control of the Air Flow Rate of Centrifugal Compressor Using State Space Method

Energy Technology Data Exchange (ETDEWEB)

Han, Jaeyoung; Jung, Mooncheong; Yu, Sangseok [Chungnam Nat’l Univ., Daejeon (Korea, Republic of); Yi, Sun [North Carolina A and T State Univ., Raleigh (United States)

2016-08-15

In this study, a model reference adaptive controller is developed to regulate the outlet air flow rate of centrifugal compressor for automotive supercharger. The centrifugal compressor is developed using the analytical based method to predict the transient behavior of operating and the designed model is validated with experimental data to confirm the system accuracy. The model reference adaptive control structure consists of a compressor model and a MRAC(model reference adaptive control) mechanism. The feedback control do not robust with variation of system parameter but the applied adaptive control is robust even if the system parameter is changed. As a result, the MRAC was regulated to reference air flow rate. Also MRAC was found to be more robust control compared with the feedback control even if the system parameter is changed.

Compound drum for a centrifugal separator

International Nuclear Information System (INIS)

1972-01-01

This invention concerns a method for centrifugal separation of UF 6 . The invention provides a composite drum capable of rapid rotation for use in a centrifugal separating arrangement for gaseous materials. The drum is provided with a first drum section comprised of a metal and a second drum section comprised of a fiber-reinforced synthetic material. The second drum section is applied on the outside peripheral surface of the first drum section, where the second drum section is provided with a number of annular components, each of which is shorter than the first drum section

Basic characteristics of centrifuges, (4)

International Nuclear Information System (INIS)

Kai, Tsunetoshi

1977-01-01

A method of numerically solving the convection-diffusion equation is presented for a binary isotopic gas mixture in the axisymmetric velocity field. A modified Newton's method is employed to perform the numerical integration without the assumptions that the pressure can be estimated from the rigid rotation model and the temperature of gas is uniform. A suitable form of the finite difference equation gives a computationally stable integration with reasonable representation of the molar concentration distribution of isotopic molecules in a rotating cylinder. The method includes a Gaussian elimination procedure which consists of the transformation of the Jacobian matrix to a triangular matrix followed by the backward elimination. Computations are made on UF 6 gas in various centrifuges which have the openings for feed, product and waste on the end plates. Discussions are also presented on gas flows and separative efficiencies for the centrifuges which have baffle plates, skirting plates and bellows. (auth.)

Microfluidic devices, systems, and methods for quantifying particles using centrifugal force

Science.gov (United States)

Schaff, Ulrich Y.; Sommer, Gregory J.; Singh, Anup K.

2015-11-17

Embodiments of the present invention are directed toward microfluidic systems, apparatus, and methods for measuring a quantity of cells in a fluid. Examples include a differential white blood cell measurement using a centrifugal microfluidic system. A method may include introducing a fluid sample containing a quantity of cells into a microfluidic channel defined in part by a substrate. The quantity of cells may be transported toward a detection region defined in part by the substrate, wherein the detection region contains a density media, and wherein the density media has a density lower than a density of the cells and higher than a density of the fluid sample. The substrate may be spun such that at least a portion of the quantity of cells are transported through the density media. Signals may be detected from label moieties affixed to the cells.

Assessing geotechnical centrifuge modelling in addressing variably saturated flow in soil and fractured rock.

Science.gov (United States)

Jones, Brendon R; Brouwers, Luke B; Van Tonder, Warren D; Dippenaar, Matthys A

2017-05-01

The vadose zone typically comprises soil underlain by fractured rock. Often, surface water and groundwater parameters are readily available, but variably saturated flow through soil and rock are oversimplified or estimated as input for hydrological models. In this paper, a series of geotechnical centrifuge experiments are conducted to contribute to the knowledge gaps in: (i) variably saturated flow and dispersion in soil and (ii) variably saturated flow in discrete vertical and horizontal fractures. Findings from the research show that the hydraulic gradient, and not the hydraulic conductivity, is scaled for seepage flow in the geotechnical centrifuge. Furthermore, geotechnical centrifuge modelling has been proven as a viable experimental tool for the modelling of hydrodynamic dispersion as well as the replication of similar flow mechanisms for unsaturated fracture flow, as previously observed in literature. Despite the imminent challenges of modelling variable saturation in the vadose zone, the geotechnical centrifuge offers a powerful experimental tool to physically model and observe variably saturated flow. This can be used to give valuable insight into mechanisms associated with solid-fluid interaction problems under these conditions. Findings from future research can be used to validate current numerical modelling techniques and address the subsequent influence on aquifer recharge and vulnerability, contaminant transport, waste disposal, dam construction, slope stability and seepage into subsurface excavations.

A Modified Conjugacy Condition and Related Nonlinear Conjugate Gradient Method

Directory of Open Access Journals (Sweden)

Shengwei Yao

2014-01-01

Full Text Available The conjugate gradient (CG method has played a special role in solving large-scale nonlinear optimization problems due to the simplicity of their very low memory requirements. In this paper, we propose a new conjugacy condition which is similar to Dai-Liao (2001. Based on this condition, the related nonlinear conjugate gradient method is given. With some mild conditions, the given method is globally convergent under the strong Wolfe-Powell line search for general functions. The numerical experiments show that the proposed method is very robust and efficient.

A gradient activation method for direct methanol fuel cells

International Nuclear Information System (INIS)

Liu, Guicheng; Yang, Zhaoyi; Halim, Martin; Li, Xinyang; Wang, Manxiang; Kim, Ji Young; Mei, Qiwen; Wang, Xindong; Lee, Joong Kee

2017-01-01

Highlights: • A gradient activation method was reported firstly for direct methanol fuel cells. • The activity recovery of Pt-based catalyst was introduced into the novel activation process. • The new activation method led to prominent enhancement of DMFC performance. • DMFC performance was improved with the novel activation step by step within 7.5 h. - Abstract: To realize gradient activation effect and recover catalytic activity of catalyst in a short time, a gradient activation method has firstly been proposed for enhancing discharge performance and perfecting activation mechanism of the direct methanol fuel cell (DMFC). This method includes four steps, i.e. proton activation, activity recovery activation, H_2-O_2 mode activation and forced discharging activation. The results prove that the proposed method has gradually realized replenishment of water and protons, recovery of catalytic activity of catalyst, establishment of transfer channels for electrons, protons, and oxygen, and optimization of anode catalyst layer for methanol transfer in turn. Along with the novel activation process going on, the DMFC discharge performance has been improved, step by step, to more than 1.9 times higher than that of the original one within 7.5 h. This method provides a practicable activation way for the real application of single DMFCs and stacks.

Flow and separation in gas centrifuge with Beams type circulation

International Nuclear Information System (INIS)

Ajsen, Eh.M.; Borisevich, V.D.; Levin, E.V.

1992-01-01

Structure of the secondary circulation flows in the working chamber of gas centrifuge for uranium isotope separation is studied using the numerical methods. Influence of the circulation thermal component on the centrifuge efficiency is analyzed. The contribution of useful component concentration difference of binary isotope mixture in feeding flows to the centrifuge efficiency is determined. Dependence of concentration optimal difference, whereby the maximum efficiency is achieved, on temperature distribution on the rotor side wall is found

Separation parameters of gas centrifuges

International Nuclear Information System (INIS)

May, W.G.

1977-01-01

Early work on development of the gas centrifuge for separation of uranium isotopes has recently been reviewed. Several configurations were investigated. The preferred configuration eventually turned out to be a countercurrent centrifuge. In this form, an internal circulation is set up, and as a consequence, light isotope concentrates at one end of the centrifuge, heavy isotope at the other. In many ways the effect resembles the separation obtained in packed columns in the chemical and petroleum industries. It is the purpose of this paper to develop this analogy between countercurrent gas centrifuges and packed towers and to illustrate its usefulness in understanding the separation process in the centrifuge. 8 figures

An approach using centrifugation for the extraction of the soil solution and its usefulness in studies of radionuclide speciation in soils - Approach using centrifugation for extraction of soil solution and its study for uranium speciation

Energy Technology Data Exchange (ETDEWEB)

Medeiros, Adriana S. [CAPES Foundation, Ministry of Education of Brazil, 70040-020, Brasilia, Brazil, Proc.BEX 1958/13-5 (Brazil); Lozano, J.C.; Prieto, C. [Universidad de Salamanca, 37008, Salamanca (Spain); Blanco Rodriguez, P.; Vera Tome, F. [Universidad de Extremadura, 06006, Badajoz (Spain)

2014-07-01

The centrifugation technique is tested as a methodology for extraction of soil solution, for further characterization, in order to elucidate its contribution to the speciation of radionuclides, particularly uranium, in radioactively contaminated soils, as well as the determination of its availability for vegetation. Centrifugation of a previously saturated soil core provides the soil solution with a specific origin inside the soil sample. In such way that the different soil solution origin, associate to the effective pressure applied to the soil core, will reflect different distribution coefficients which affect the radionuclide availability definition. Speciation of radionuclides in the soil solution can be also conditioned by this water origin. The development of this methodology relating to technical challenges faces materials suitable for the centrifugation process, both in terms of mechanical properties and chemical inertness. This paper reports the preparation of ceramic pellets of perlite produced with the intention of replacing glass pellets, used inserts in support to soils coupled with centrifuges. The characterization of porosity and the test of its chemical inertness and mechanical strength to the centrifugation process have been performed. Porosity characterization is required to control the saturation gradient, which conditions the flow of water from the soil. Its mechanical adequacy was tested by subjecting the pellets to the centrifugation process and assessing its integrity end. Chemical inertia was measured by placing the tablets in aqueous solutions of known composition and then evaluating the presence or absence of elements in this solution, after on time of contact between them. (authors)

New, rapid method to measure dissolved silver concentration in silver nanoparticle suspensions by aggregation combined with centrifugation

International Nuclear Information System (INIS)

Dong, Feng; Valsami-Jones, Eugenia; Kreft, Jan-Ulrich

2016-01-01

It is unclear whether the antimicrobial activities of silver nanoparticles (AgNPs) are exclusively mediated by the release of silver ions (Ag"+) or, instead, are due to combined nanoparticle and silver ion effects. Therefore, it is essential to quantify dissolved Ag in nanosilver suspensions for investigations of nanoparticle toxicity. We developed a method to measure dissolved Ag in Ag"+/AgNPs mixtures by combining aggregation of AgNPs with centrifugation. We also describe the reproducible synthesis of stable, uncoated AgNPs. Uncoated AgNPs were quickly aggregated by 2 mM Ca"2"+, forming large clusters that could be sedimented in a low-speed centrifuge. At 20,100g, the sedimentation time of AgNPs was markedly reduced to 30 min due to Ca"2"+-mediated aggregation, confirmed by the measurements of Ag content in supernatants with graphite furnace atomic absorption spectrometry. No AgNPs were detected in the supernatant by UV–Vis absorption spectra after centrifuging the aggregates. Our approach provides a convenient and inexpensive way to separate dissolved Ag from AgNPs, avoiding long ultracentrifugation times or Ag"+ adsorption to ultrafiltration membranes.

New, rapid method to measure dissolved silver concentration in silver nanoparticle suspensions by aggregation combined with centrifugation

Energy Technology Data Exchange (ETDEWEB)

Dong, Feng, E-mail: fengdongub@gmail.com [University of Birmingham, Institute of Microbiology and Infection, School of Biosciences (United Kingdom); Valsami-Jones, Eugenia [University of Birmingham, School of Geography, Earth and Environmental Sciences (United Kingdom); Kreft, Jan-Ulrich [University of Birmingham, Institute of Microbiology and Infection, School of Biosciences (United Kingdom)

2016-09-15

It is unclear whether the antimicrobial activities of silver nanoparticles (AgNPs) are exclusively mediated by the release of silver ions (Ag{sup +}) or, instead, are due to combined nanoparticle and silver ion effects. Therefore, it is essential to quantify dissolved Ag in nanosilver suspensions for investigations of nanoparticle toxicity. We developed a method to measure dissolved Ag in Ag{sup +}/AgNPs mixtures by combining aggregation of AgNPs with centrifugation. We also describe the reproducible synthesis of stable, uncoated AgNPs. Uncoated AgNPs were quickly aggregated by 2 mM Ca{sup 2+}, forming large clusters that could be sedimented in a low-speed centrifuge. At 20,100g, the sedimentation time of AgNPs was markedly reduced to 30 min due to Ca{sup 2+}-mediated aggregation, confirmed by the measurements of Ag content in supernatants with graphite furnace atomic absorption spectrometry. No AgNPs were detected in the supernatant by UV–Vis absorption spectra after centrifuging the aggregates. Our approach provides a convenient and inexpensive way to separate dissolved Ag from AgNPs, avoiding long ultracentrifugation times or Ag{sup +} adsorption to ultrafiltration membranes.

Application of Conjugate Gradient methods to tidal simulation

Science.gov (United States)

Barragy, E.; Carey, G.F.; Walters, R.A.

1993-01-01

A harmonic decomposition technique is applied to the shallow water equations to yield a complex, nonsymmetric, nonlinear, Helmholtz type problem for the sea surface and an accompanying complex, nonlinear diagonal problem for the velocities. The equation for the sea surface is linearized using successive approximation and then discretized with linear, triangular finite elements. The study focuses on applying iterative methods to solve the resulting complex linear systems. The comparative evaluation includes both standard iterative methods for the real subsystems and complex versions of the well known Bi-Conjugate Gradient and Bi-Conjugate Gradient Squared methods. Several Incomplete LU type preconditioners are discussed, and the effects of node ordering, rejection strategy, domain geometry and Coriolis parameter (affecting asymmetry) are investigated. Implementation details for the complex case are discussed. Performance studies are presented and comparisons made with a frontal solver. ?? 1993.

Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer

Science.gov (United States)

Burtis, C.A.; Johnson, W.F.; Walker, W.A.

1985-08-05

A rotor and disc assembly for use in a centrifugal fast analyzer. The assembly is designed to process multiple samples of whole blood followed by aliquoting of the resultant serum into precisely measured samples for subsequent chemical analysis. The assembly requires minimal operator involvement with no mechanical pipetting. The system comprises: (1) a whole blood sample disc; (2) a serum sample disc; (3) a sample preparation rotor; and (4) an analytical rotor. The blood sample disc and serum sample disc are designed with a plurality of precision bore capillary tubes arranged in a spoked array. Samples of blood are loaded into the blood sample disc by capillary action and centrifugally discharged into cavities of the sample preparation rotor where separation of serum and solids is accomplished. The serum is loaded into the capillaries of the serum sample disc by capillary action and subsequently centrifugally expelled into cuvettes of the analyticaly rotor for conventional methods. 5 figs.

Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer

Science.gov (United States)

Burtis, Carl A.; Johnson, Wayne F.; Walker, William A.

1988-01-01

A rotor and disc assembly for use in a centrifugal fast analyzer. The assembly is designed to process multiple samples of whole blood followed by aliquoting of the resultant serum into precisely measured samples for subsequent chemical analysis. The assembly requires minimal operator involvement with no mechanical pipetting. The system comprises (1) a whole blood sample disc, (2) a serum sample disc, (3) a sample preparation rotor, and (4) an analytical rotor. The blood sample disc and serum sample disc are designed with a plurality of precision bore capillary tubes arranged in a spoked array. Samples of blood are loaded into the blood sample disc in capillary tubes filled by capillary action and centrifugally discharged into cavities of the sample preparation rotor where separation of serum and solids is accomplished. The serum is loaded into the capillaries of the serum sample disc by capillary action and subsequently centrifugally expelled into cuvettes of the analytical rotor for analysis by conventional methods.

Differential white cell count by centrifugal microfluidics.

Energy Technology Data Exchange (ETDEWEB)

Sommer, Gregory Jon; Tentori, Augusto M.; Schaff, Ulrich Y.

2010-07-01

We present a method for counting white blood cells that is uniquely compatible with centrifugation based microfluidics. Blood is deposited on top of one or more layers of density media within a microfluidic disk. Spinning the disk causes the cell populations within whole blood to settle through the media, reaching an equilibrium based on the density of each cell type. Separation and fluorescence measurement of cell types stained with a DNA dye is demonstrated using this technique. The integrated signal from bands of fluorescent microspheres is shown to be proportional to their initial concentration in suspension. Among the current generation of medical diagnostics are devices based on the principle of centrifuging a CD sized disk functionalized with microfluidics. These portable 'lab on a disk' devices are capable of conducting multiple assays directly from a blood sample, embodied by platforms developed by Gyros, Samsung, and Abaxis. [1,2] However, no centrifugal platform to date includes a differential white blood cell count, which is an important metric complimentary to diagnostic assays. Measuring the differential white blood cell count (the relative fraction of granulocytes, lymphocytes, and monocytes) is a standard medical diagnostic technique useful for identifying sepsis, leukemia, AIDS, radiation exposure, and a host of other conditions that affect the immune system. Several methods exist for measuring the relative white blood cell count including flow cytometry, electrical impedance, and visual identification from a stained drop of blood under a microscope. However, none of these methods is easily incorporated into a centrifugal microfluidic diagnostic platform.

A centrifuge CO2 pellet cleaning system

International Nuclear Information System (INIS)

Foster, C.A.; Fisher, P.W.; Nelson, W.D.; Schechter, D.E.

1993-01-01

Centrifuge-based cryogenic pellet accelerator technology, originally developed at Oak Ridge National Laboratory (ORNL) for the purpose of refueling fusion reactors with high-speed pellets of frozen deuterium/tritium,is now being developed as a method of cleaning without the use of conventional solvents. In these applications large quantities of pellets made of frozen CO 2 or argon are accelerated in a high-speed rotor. The accelerated pellet stream is used to clean or etch surfaces. The advantage of this system is that the spent pellets and debris resulting from the cleaning process can be filtered leaving only the debris for disposal. This paper discusses the centrifuge CO 2 pellet cleaning system, the physics model of the pellet impacting the surface, the centrifuge apparatus, and some initial cleaning and etching tests

Comparison of PCR-Based Diagnosis with Centrifuged-Based Enrichment Method for Detection of Borrelia Persica in Animal Blood Samples

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SR Naddaf

2011-06-01

Full Text Available Background: The mainstay of diagnosis of relapsing fever (RF is demonstration of the spirochetes in Giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by mi­cros­copy. This study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spi­ro­chetemia. Methods: Animal blood samples with low degrees of spirochetemia were tested with two PCRs and a nested PCR tar­get­ing flaB, GlpQ, and rrs genes. Also, a centrifuged-based enrichment method and Giemsa staining were per­formed on blood samples with various degrees of spirochetemia. Results: The flaB-PCR and nested rrs-PCR turned positive with various degrees of spirochetemia including the blood samples that turned negative with dark-field microscopy. The GlpQ-PCR was positive as far as at least one spi­ro­chete was seen in 5-10 microscopic fields. The sensitivity of GlpQ-PCR increased when DNA from Buffy Coat Layer (BCL was used as template. The centrifuged-based enrichment method turned positive with as low concentra­tion as 50 bacteria/ml blood, while Giemsa thick staining detected bacteria with concentrations ≥ 25000 bacteria/ml. Conclusion: Centrifuged-based enrichment method appeared as much as 500-fold more sensitive than thick smears, which makes it even superior to some PCR assays. Due to simplicity and minimal laboratory requirements, this method can be considered a valuable tool for diagnosis of RF in rural health centers.

Comparison of PCR-Based Diagnosis with Centrifuged-Based Enrichment Method for Detection of Borrelia persica in Animal Blood Samples

Directory of Open Access Journals (Sweden)

SR Naddaf

2011-06-01

Background: The mainstay of diagnosis of relapsing fever (RF is demonstration of the spirochetes in Giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by mi­cros­copy. This study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spi­ro­chetemia. Methods: Animal blood samples with low degrees of spirochetemia were tested with two PCRs and a nested PCR tar­get­ing flaB, GlpQ, and rrs genes. Also, a centrifuged-based enrichment method and Giemsa staining were per­formed on blood samples with various degrees of spirochetemia. Results: The flaB-PCR and nested rrs-PCR turned positive with various degrees of spirochetemia including the blood samples that turned negative with dark-field microscopy. The GlpQ-PCR was positive as far as at least one spi­ro­chete was seen in 5-10 microscopic fields. The sensitivity of GlpQ-PCR increased when DNA from Buffy Coat Layer (BCL was used as template. The centrifuged-based enrichment method turned positive with as low concentra­tion as 50 bacteria/ml blood, while Giemsa thick staining detected bacteria with concentrations ≥ 25000 bacteria/ml.  Conclusion: Centrifuged-based enrichment method appeared as much as 500-fold more sensitive than thick smears, which makes it even superior to some PCR assays. Due to simplicity and minimal laboratory requirements, this method can be considered a valuable tool for diagnosis of RF in rural health centers.  

New centrifugation blood culture device.

Science.gov (United States)

Dorn, G L; Smith, K

1978-01-01

A single-tube blood culture device designed for centrifugation in a tabletop centrifuge is described. Reconstruction experiments using 21 different organisms and human donor blood indicate that excellent recovery can be obtained by centrifugation for 30 min at 3,000 X g. PMID:342539

Uranium enrichment in Europe by the gas centrifuge process

International Nuclear Information System (INIS)

Severin, D.J.E.

1975-01-01

To begin with, this lesson gives an outline of the expected energy demand of the Western World and the concentration of the European companies participating in uranium enrichment by the gas centrifuge method. Next, a) the principles of the gas centrifuge method are outlined, b) its advantages over other industrial processes are stressed, and c) the characteristic data of complete plants are given. The existing German, Dutch, and British pilot plants are mentioned as examples for the perfected state of the process. The Capenhurst (UK) and Almedo (NL) demonstration plants, each with a capacity of 200 t SW/a, will have been extended to 2 x 1.000 t SW/a by 1982. Finally, economic data of the gas centrifuge process are given. The term 'separative work' is explained in an annex. (GG) [de

Enrichment technology. Dependable vendor of gas centrifuges

International Nuclear Information System (INIS)

Anon.

2011-01-01

Enrichment Technology is an innovative, high-tech company that develops, manufactures and installs gas centrifuges for enriching uranium. In addition, Enrichment Technology designs enrichment plants that use gas centrifuge technology. This technology offers the most efficient and cost-effective method for enriching uranium yet: high-performance, safe technology that dominates the market with a global share of 45 percent. A determining factor in Enrichment Technology's success is its mission: supplying its customers with safe, reliable technology. Production of the centrifuges requires versatile know-how and collaboration between different departments as well as interdisciplinary teams at the various sites. More than 2000 operators at 8 sites in 5 countries contribute their individual knowledge and personal skills in order to produce this exceptional technology. The head office is in Beaconsfield near London and the operational headquarters are in Almelo in the Netherlands. There are other sites in Germany (Juelich und Gronau), Great Britain (Capenhurst) as well as project sites in the USA and France. Capenhurst is where experienced engineers design new enrichment plants and organise their construction. Centrifuge components are manufactured in Almelo and Juelich, while the pipework needed to connect up the centrifuges is produced at the site in Gronau. In Juelich, highly qualified scientists in interdisciplinary teams are continuously researching ways of improving the current centrifuges. Communication between specialists in the fields of chemistry, physics and engineering forms the basis for the company's success and the key to extending this leading position in the global enrichment market. (orig.)

The commercial role for centrifuge enrichment

International Nuclear Information System (INIS)

Readle, P.H.; Wilcox, P.

1987-01-01

The enrichment market is extremely competitive and capacity greatly exceeds demand. BNFL [British Nuclear Fuels Ltd.] is in a unique position in having commercial experience of the two enrichment technologies currently used industrially: diffusion, and centrifuge enrichment through its associate company Urenco. In addition, BNFL is developing laser enrichment techniques as part of a UK development programme. The paper describes the enrichment market, briefly discusses the relative merits of the various methods of uranium enrichment and concludes that the gas centrifuge will be best able to respond to market needs for at least the remainder of the century. (author)

PRECONDITIONED BI-CONJUGATE GRADIENT METHOD FOR RADIATIVE TRANSFER IN SPHERICAL MEDIA

International Nuclear Information System (INIS)

Anusha, L. S.; Nagendra, K. N.; Paletou, F.; Leger, L.

2009-01-01

A robust numerical method called the Preconditioned Bi-Conjugate Gradient (Pre-BiCG) method is proposed for the solution of the radiative transfer equation in spherical geometry. A variant of this method called Stabilized Preconditioned Bi-Conjugate Gradient (Pre-BiCG-STAB) is also presented. These are iterative methods based on the construction of a set of bi-orthogonal vectors. The application of the Pre-BiCG method in some benchmark tests shows that the method is quite versatile, and can handle difficult problems that may arise in astrophysical radiative transfer theory.

76 FR 50767 - In the Matter of USEC Inc., American Centrifuge Lead Cascade Facility, and American Centrifuge...

Science.gov (United States)

2011-08-16

...; License Nos. SNM-7003, SNM-2011] In the Matter of USEC Inc., American Centrifuge Lead Cascade Facility, and American Centrifuge Plant; Order Extending the Date by Which the Direct Transfer of Licenses Is To... American Centrifuge Lead Cascade Facility (Lead Cascade) and American Centrifuge Plant (ACP), respectively...

Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human and Amphiuma red blood cells.

Science.gov (United States)

Hoffman, Joseph F; Inoué, Shinya

2006-02-21

This paper describes changes that occur in human and Amphiuma red blood cells observed during centrifugation with a special microscope. Dilute suspensions of cells were layered, in a centrifuge chamber, above an osmotically matched dense solution, containing Nycodenz, Ficoll, or Percoll (Pharmacia) that formed a density gradient that allowed the cells to slowly settle to an equilibrium position. Biconcave human red blood cells moved downward at low forces with minimum wobble. The cells oriented vertically when the force field was increased and Hb sedimented as the lower part of each cell became bulged and assumed a "bag-like" shape. The upper centripetal portion of the cell became thinner and remained biconcave. These changes occurred rapidly and were completely reversible upon lowering the centrifugal force. Bag-shaped cells, upon touching red cells in rouleau, immediately reverted to biconcave disks as they flipped onto a stack. Amphiuma red cells displayed a different type of reversible stratification and deformation at high force fields. Here the cells became stretched, with the nucleus now moving centrifugally, the Hb moving centripetally, and the bottom of the cells becoming thinner and clear. Nevertheless, the distribution of the marginal bands at the cells' rim was unchanged. We conclude that centrifugation, per se, while changing a red cell's shape and the distribution of its intracellular constituents, does so in a completely reversible manner. Centrifugation of red cells harboring altered or missing structural elements could provide information on shape determinants that are still unexplained.

Designing and analysis study of uranium enrichment with gas centrifuge

International Nuclear Information System (INIS)

Tsunetoshi Kai

2006-01-01

This note concerns a designing and analysis study of uranium enrichment with a gas centrifuge. At first, one dimensional model is presented and a conventional analytical method is applied to grasp the general idea of a centrifuge performance. Secondly, two-dimensional numerical method is adopted to describe the diffusion phenomena with assumption of simple flow patterns. Parametric surveys are made on the dimension of a centrifuge rotor, the gas feed, withdrawal and circulation system, and operation variables such as feed flow rate, cut and so on. Thirdly, full numerical solutions are obtained for the flow and diffusion equations in static state, using a modified version of the Newton method without neglect of any non-linear term. The numerical results are compared with the experimental data made by Beams et al. and Zippe, and found to be in good agreement. Further, the theoretical pressure and separative power are compared respectively with experimental ones on a comparatively recent centrifuge. The results reveal that the characteristics of separation performance of a centrifuge can be fully described by the present method. Some of inevitable problems are tackled regarding UF 6 gas isotope separation by centrifugation. To examine the influence of the extraneous light gas, the diffusion equations for ternary mixture are solved and also the flow field of binary mixture with large mass difference is obtained to simultaneously solve the Navier-Stokes equations and the diffusion equation.for binary case. Since the gas in the interior region of the rotor is so rarefied that the Navier-Stokes equations cease to be valid, the Burnett equations are solved.for gas flow in a rotating cylinder. Considering that the uranium recovered at a reprocessing plant includes 236 U besides 235 U and 238 U, the concentration distributions of the ternary gas isotopes are determined and a value function is defined for the evaluation of separative work for the multi-component mixture

A hybrid optimization method for biplanar transverse gradient coil design

International Nuclear Information System (INIS)

Qi Feng; Tang Xin; Jin Zhe; Jiang Zhongde; Shen Yifei; Meng Bin; Zu Donglin; Wang Weimin

2007-01-01

The optimization of transverse gradient coils is one of the fundamental problems in designing magnetic resonance imaging gradient systems. A new approach is presented in this paper to optimize the transverse gradient coils' performance. First, in the traditional spherical harmonic target field method, high order coefficients, which are commonly ignored, are used in the first stage of the optimization process to give better homogeneity. Then, some cosine terms are introduced into the series expansion of stream function. These new terms provide simulated annealing optimization with new freedoms. Comparison between the traditional method and the optimized method shows that the inhomogeneity in the region of interest can be reduced from 5.03% to 1.39%, the coil efficiency increased from 3.83 to 6.31 mT m -1 A -1 and the minimum distance of these discrete coils raised from 1.54 to 3.16 mm

Fabrication of silk fibroin film using centrifugal casting technique for corneal tissue engineering.

Science.gov (United States)

Lee, Min Chae; Kim, Dong-Kyu; Lee, Ok Joo; Kim, Jung-Ho; Ju, Hyung Woo; Lee, Jung Min; Moon, Bo Mi; Park, Hyun Jung; Kim, Dong Wook; Kim, Su Hyeon; Park, Chan Hum

2016-04-01

Films prepared from silk fibroin have shown potential as biomaterials in tissue engineering applications for the eye. Here, we present a novel process for fabrication of silk fibroin films for corneal application. In this work, fabrication of silk fibroin films was simply achieved by centrifugal force. In contrast to the conventional dry casting method, we carried out the new process in a centrifuge with a rotating speed of 4000 rpm, where centrifugal force was imposed on an aluminum tube containing silk fibroin solution. In the present study, we also compared the surface roughness, mechanical properties, transparency, and cell proliferation between centrifugal and dry casting method. In terms of surface morphology, films fabricated by the centrifugal casting have less surface roughness than those by the dry casting. For elasticity and transparency, silk fibroin films obtained from the centrifugal casting had favorable results compared with those prepared by dry casting. Furthermore, primary human corneal keratocytes grew better in films prepared by the centrifugal casting. Therefore, our results suggest that this new fabrication process for silk fibroin films offers important potential benefits for corneal tissue regeneration. © 2015 Wiley Periodicals, Inc.

A novel technique using the Hendrickx centrifuge for extracting winter sporangia of Synchytrium endobioticum from soil

NARCIS (Netherlands)

Wander, J.G.N.; Berg, van den W.; Boogert, van den P.H.J.F.; Lamers, J.G.; Leeuwen, van G.C.M.; Hendrickx, G.; Bonants, P.J.M.

2007-01-01

A zonal centrifugation method, known as the Hendrickx centrifuge technique, was tested for routine detection of winter sporangia of Synchytrium endobioticum in soil. In four experiments the ability of the Hendrickx centrifuge to extract the sporangia from soil was compared with a method used by the

Preflight screening techniques for centrifuge-simulated suborbital spaceflight.

Science.gov (United States)

Pattarini, James M; Blue, Rebecca S; Castleberry, Tarah L; Vanderploeg, James M

2014-12-01

Historically, space has been the venue of the healthy individual. With the advent of commercial spaceflight, we face the novel prospect of routinely exposing spaceflight participants (SPFs) with multiple comorbidities to the space environment. Preflight screening procedures must be developed to identify those individuals at increased risk during flight. We examined the responses of volunteers to centrifuge accelerations mimicking commercial suborbital spaceflight profiles to evaluate how potential SFPs might tolerate such forces. We evaluated our screening process for medical approval of subjects for centrifuge participation for applicability to commercial spaceflight operations. All registered subjects completed a medical questionnaire, physical examination, and electrocardiogram. Subjects with identified concerns including cardiopulmonary disease, hypertension, and diabetes were required to provide documentation of their conditions. There were 335 subjects who registered for the study, 124 who completed all prescreening, and 86 subjects who participated in centrifuge trials. Due to prior medical history, five subjects were disqualified, most commonly for psychiatric reasons or uncontrolled medical conditions. Of the subjects approved, four individuals experienced abnormal physiological responses to centrifuge profiles, including one back strain and three with anxiety reactions. The screening methods used were judged to be sufficient to identify individuals physically capable of tolerating simulated suborbital flight. Improved methods will be needed to identify susceptibility to anxiety reactions. While severe or uncontrolled disease was excluded, many subjects successfully participated in centrifuge trials despite medical histories of disease that are disqualifying under historical spaceflight screening regimes. Such screening techniques are applicable for use in future commercial spaceflight operations.

Methods for Fabricating Gradient Alloy Articles with Multi-Functional Properties

Science.gov (United States)

Hofmann, Douglas C. (Inventor); Borgonia, John Paul C. (Inventor); Dillon, Robert P. (Inventor); Suh, Eric J. (Inventor); Mulder, Jerry L. (Inventor); Gardner, Paul B. (Inventor)

2015-01-01

Systems and methods for fabricating multi-functional articles comprised of additively formed gradient materials are provided. The fabrication of multi-functional articles using the additive deposition of gradient alloys represents a paradigm shift from the traditional way that metal alloys and metal/metal alloy parts are fabricated. Since a gradient alloy that transitions from one metal to a different metal cannot be fabricated through any conventional metallurgy techniques, the technique presents many applications. Moreover, the embodiments described identify a broad range of properties and applications.

The Effect of Moment of Inertia on the Liquids in Centrifugal Microfluidics

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Esmail Pishbin

2016-12-01

Full Text Available The flow of liquids in centrifugal microfluidics is unidirectional and dominated by centrifugal and Coriolis forces (i.e., effective only at T-junctions. Developing mechanisms and discovering efficient techniques to propel liquids in any direction other than the direction of the centrifugal force has been the subject of a large number of studies. The capillary force attained by specific surface treatments, pneumatic energy, active and passive flow reciprocation and Euler force have been previously introduced in order to manipulate the liquid flow and push it against the centrifugal force. Here, as a new method, the moment of inertia of the liquid inside a chamber in a centrifugal microfluidic platform is employed to manipulate the flow and propel the liquid passively towards the disc center. Furthermore, the effect of the moment of inertia on the liquid in a rectangular chamber is evaluated, both in theory and experiments, and the optimum geometry is defined. As an application of the introduced method, the moment of inertia of the liquid is used in order to mix two different dyed deionized (DI waters; the mixing efficiency is evaluated and compared to similar mixing techniques. The results show the potential of the presented method for pumping liquids radially inward with relatively high flow rates (up to 23 mm3/s and also efficient mixing in centrifugal microfluidic platforms.

Water Drainage from Unsaturated Soils in a Centrifuge Permeameter

Science.gov (United States)

Ornelas, G.; McCartney, J.; Zhang, M.

2013-12-01

This study involves an analysis of water drainage from an initially saturated silt layer in a centrifuge permeameter to evaluate the hydraulic properties of the soil layer in unsaturated conditions up to the point where the water phase becomes discontinuous. These properties include the soil water retention curve (SWRC) and the hydraulic conductivity function (HCF). The hydraulic properties of unsaturated silt are used in soil-atmosphere interaction models that take into account the role of infiltration and evaporation of water from soils due to atmospheric interaction. These models are often applied in slope stability analyses, landfill cover design, aquifer recharge analyses, and agricultural engineering. The hydraulic properties are also relevant to recent research concerning geothermal heating and cooling, as they can be used to assess the insulating effects of soil around underground heat exchangers. This study employs a high-speed geotechnical centrifuge to increase the self-weight of a compacted silt specimen atop a filter plate. Under a centrifuge acceleration of N times earth's gravity, the concept of geometric similitude indicates that the water flow process in a small-scale soil layer will be similar to those in a soil layer in the field that is N times thicker. The centrifuge acceleration also results in an increase in the hydraulic gradient across the silt specimen, which causes water to flow out of the pores following Darcy's law. The drainage test was performed until the rate of liquid water flow out of the soil layer slowed to a negligible level, which corresponds to the transition point at which further water flow can only occur due to water vapor diffusion following Fick's law. The data from the drainage test in the centrifuge were used to determine the SWRC and HCF at different depths in the silt specimen, which compared well with similar properties defined using other laboratory tests. The transition point at which liquid water flow stopped (and

Isolation of human salivary extracellular vesicles by iodixanol density gradient ultracentrifugation and their characterizations

Directory of Open Access Journals (Sweden)

Kazuya Iwai

2016-05-01

Full Text Available Diagnostic methods that focus on the extracellular vesicles (EVs present in saliva have been attracting great attention because of their non-invasiveness. EVs contain biomolecules such as proteins, messenger RNA (mRNA and microRNA (miRNA, which originate from cells that release EVs, making them an ideal source for liquid biopsy. Although there have been many reports on density-based fractionation of EVs from blood and urine, the number of reports on EVs from saliva has been limited, most probably because of the difficulties in separating EVs from viscous saliva using density gradient centrifugation. This article establishes a protocol for the isolation of EVs from human saliva using density gradient centrifugation. The fractionated salivary EVs were characterized by atomic force microscopy, western blot and reverse transcription polymerase chain reaction. The results indicate that salivary EVs have a smaller diameter (47.8±12.3 nm and higher density (1.11 g/ml than EVs isolated from conditioned cell media (74.0±23.5 nm and 1.06 g/ml, respectively. Additionally, to improve the throughput of density-based fractionation of EVs, the original protocol was further modified by using a fixed angle rotor instead of a swinging rotor. It was also confirmed that several miRNAs were expressed strongly in the EV-marker-expressing fractions.

Centrifuge design and development

International Nuclear Information System (INIS)

Edwards, T.T.; Holmes, M.D.

1987-01-01

BNFL has been enriching uranium on an industrial scale using the centrifuge process for over a decade. Together with its Urenco partners, a joint development programme has been and is being vigorously pursued to reduce specific costs, increase output and maintain competitiveness throughout the 1990s. The paper summarises the development of the centrifuge from its earliest concepts through to the centrifuges of today which are jointly designed by the Urenco partners. The potential for further development is also examined. (author)

A rapid method for estimation of Pu-isotopes in urine samples using high volume centrifuge.

Science.gov (United States)

Kumar, Ranjeet; Rao, D D; Dubla, Rupali; Yadav, J R

2017-07-01

The conventional radio-analytical technique used for estimation of Pu-isotopes in urine samples involves anion exchange/TEVA column separation followed by alpha spectrometry. This sequence of analysis consumes nearly 3-4 days for completion. Many a times excreta analysis results are required urgently, particularly under repeat and incidental/emergency situations. Therefore, there is need to reduce the analysis time for the estimation of Pu-isotopes in bioassay samples. This paper gives the details of standardization of a rapid method for estimation of Pu-isotopes in urine samples using multi-purpose centrifuge, TEVA resin followed by alpha spectrometry. The rapid method involves oxidation of urine samples, co-precipitation of plutonium along with calcium phosphate followed by sample preparation using high volume centrifuge and separation of Pu using TEVA resin. Pu-fraction was electrodeposited and activity estimated using 236 Pu tracer recovery by alpha spectrometry. Ten routine urine samples of radiation workers were analyzed and consistent radiochemical tracer recovery was obtained in the range 47-88% with a mean and standard deviation of 64.4% and 11.3% respectively. With this newly standardized technique, the whole analytical procedure is completed within 9h (one working day hour). Copyright © 2017 Elsevier Ltd. All rights reserved.

Simulations of overall flow in gas centrifuge considering feed jet

International Nuclear Information System (INIS)

He Liang; Jiang Dongjun; Ying Chuntong

2010-01-01

A coupled method for the numerical solution of the flow in rapidly rotating gas centrifuge was presented. An iteration process of DSMC and CFD was performed to analyze the overall flow in radial direction, in which DSMC was adopted to simulate the rarefied region, and CFD was adopted to the counter-current of gas centrifuge to discrete the model equations. It was applied to simulate the 2D symmetrical flow model considering the rarefied region with the feed jet flow. A series of illustrative numerical examples were given. The flow structures of the feed jet in the rarefied gas flow region were shown. The results suggest that DSMC CFD coupled method is competent to the simulations of overall flow in a gas centrifuge. (authors)

Centrifugal washing and recovery as an improved method for obtaining lignin precipitated from South African kraft mill black liquor

CSIR Research Space (South Africa)

Namane, M

2015-10-01

Full Text Available This study describes centrifugal recovery as an improved method for collection of lignin isolated from black liquor obtained from a South African kraft mill. Precipitation of lignin was achieved by utilising 6 M sulphuric acid. Recovery...

Vacuum chamber-free centrifuge with magnetic bearings.

Science.gov (United States)

Park, Cheol Hoon; Kim, Soohyun; Kim, Kyung-Soo

2013-09-01

Centrifuges are devices that separate particles of different densities and sizes through the application of a centrifugal force. If a centrifuge could be operated under atmospheric conditions, all vacuum-related components such as the vacuum chamber, vacuum pump, diffusion pump, and sealing could be removed from a conventional centrifuge system. The design and manufacturing procedure for centrifuges could then be greatly simplified to facilitate the production of lightweight centrifuge systems of smaller volume. Furthermore, the maintenance costs incurred owing to wear and tear due to conventional ball bearings would be eliminated. In this study, we describe a novel vacuum chamber-free centrifuge supported by magnetic bearings. We demonstrate the feasibility of the vacuum chamber-free centrifuge by presenting experimental results that verify its high-speed support capability and motoring power capacity.

Flow control arrangements for centrifuges

International Nuclear Information System (INIS)

Alderton, G.W.; Davidge, P.C.

1983-01-01

In a centrifuge plant for the separation of uranium isotopes, when a centrifuge machine breaks down, light gas is produced. This gas can cause adjacent machines to break down, so propagating the fault. The present invention provides flow control arrangements in gas pipes to the centrifuge, whereby sudden egress of gas from a failed machine is inhibited. (author)

Monte Carlo method for polarized radiative transfer in gradient-index media

International Nuclear Information System (INIS)

Zhao, J.M.; Tan, J.Y.; Liu, L.H.

2015-01-01

Light transfer in gradient-index media generally follows curved ray trajectories, which will cause light beam to converge or diverge during transfer and induce the rotation of polarization ellipse even when the medium is transparent. Furthermore, the combined process of scattering and transfer along curved ray path makes the problem more complex. In this paper, a Monte Carlo method is presented to simulate polarized radiative transfer in gradient-index media that only support planar ray trajectories. The ray equation is solved to the second order to address the effect induced by curved ray trajectories. Three types of test cases are presented to verify the performance of the method, which include transparent medium, Mie scattering medium with assumed gradient index distribution, and Rayleigh scattering with realistic atmosphere refractive index profile. It is demonstrated that the atmospheric refraction has significant effect for long distance polarized light transfer. - Highlights: • A Monte Carlo method for polarized radiative transfer in gradient index media. • Effect of curved ray paths on polarized radiative transfer is considered. • Importance of atmospheric refraction for polarized light transfer is demonstrated

Evaluation of a reduced centrifugation time and higher centrifugal force on various general chemistry and immunochemistry analytes in plasma and serum.

Science.gov (United States)

Møller, Mette F; Søndergaard, Tove R; Kristensen, Helle T; Münster, Anna-Marie B

2017-09-01

Background Centrifugation of blood samples is an essential preanalytical step in the clinical biochemistry laboratory. Centrifugation settings are often altered to optimize sample flow and turnaround time. Few studies have addressed the effect of altering centrifugation settings on analytical quality, and almost all studies have been done using collection tubes with gel separator. Methods In this study, we compared a centrifugation time of 5 min at 3000 ×  g to a standard protocol of 10 min at 2200 ×  g. Nine selected general chemistry and immunochemistry analytes and interference indices were studied in lithium heparin plasma tubes and serum tubes without gel separator. Results were evaluated using mean bias, difference plots and coefficient of variation, compared with maximum allowable bias and coefficient of variation used in laboratory routine quality control. Results For all analytes except lactate dehydrogenase, the results were within the predefined acceptance criteria, indicating that the analytical quality was not compromised. Lactate dehydrogenase showed higher values after centrifugation for 5 min at 3000 ×  g, mean bias was 6.3 ± 2.2% and the coefficient of variation was 5%. Conclusions We found that a centrifugation protocol of 5 min at 3000 ×  g can be used for the general chemistry and immunochemistry analytes studied, with the possible exception of lactate dehydrogenase, which requires further assessment.

A critical analysis of some popular methods for the discretisation of the gradient operator in finite volume methods

Science.gov (United States)

Syrakos, Alexandros; Varchanis, Stylianos; Dimakopoulos, Yannis; Goulas, Apostolos; Tsamopoulos, John

2017-12-01

Finite volume methods (FVMs) constitute a popular class of methods for the numerical simulation of fluid flows. Among the various components of these methods, the discretisation of the gradient operator has received less attention despite its fundamental importance with regards to the accuracy of the FVM. The most popular gradient schemes are the divergence theorem (DT) (or Green-Gauss) scheme and the least-squares (LS) scheme. Both are widely believed to be second-order accurate, but the present study shows that in fact the common variant of the DT gradient is second-order accurate only on structured meshes whereas it is zeroth-order accurate on general unstructured meshes, and the LS gradient is second-order and first-order accurate, respectively. This is explained through a theoretical analysis and is confirmed by numerical tests. The schemes are then used within a FVM to solve a simple diffusion equation on unstructured grids generated by several methods; the results reveal that the zeroth-order accuracy of the DT gradient is inherited by the FVM as a whole, and the discretisation error does not decrease with grid refinement. On the other hand, use of the LS gradient leads to second-order accurate results, as does the use of alternative, consistent, DT gradient schemes, including a new iterative scheme that makes the common DT gradient consistent at almost no extra cost. The numerical tests are performed using both an in-house code and the popular public domain partial differential equation solver OpenFOAM.

Method to stimulate dose gradient in liquid media

International Nuclear Information System (INIS)

Scarlat, F.

1993-01-01

The depth absorbed dose from electrons with energy higher than 10 MeV shows a distribution with a big-percentage absorbed dose at the entrance surface and a small dose gradient. This is due to the big distance between the virtual focus and irradiated liquid medium. In order to stimulate dose gradient and decrease the surface dose, this paper presents a method for obtaining the second focus by means of a magnetostatic planar wiggler. Preliminary calculations indicated that the absorbed dose rate increases two-three times at the reference plane in the irradiated liquid medium. (Author)

Performance/Noise Optimization of Centrifugal Fan Using Response Surface Method

Energy Technology Data Exchange (ETDEWEB)

Shin, Donghui; Cheong, Cheolung [Pusan Nat’l Univ., Busan (Korea, Republic of); Heo Seung [Korea Aerospace Industries, Sacheon (Korea, Republic of); Kim, Tae-Hoon; Jung, Jiwon [LG Electronics, Seoul (Korea, Republic of)

2017-03-15

In this study, centrifugal fan blades used to circulate cold air inside a household refrigerator were optimized to achieve high performance and low noise by using the response surface method, which is frequently employed as an optimization algorithm when multiple independent variables affect one dependent variable. The inlet and outlet blade angles, and the inner radius, were selected as the independent variables. First, the fan blades were optimized to achieve the maximum volume flow rate. Based on this result, a prototype fan blade was manufactured using a 3-D printer. The measured P-Q curves confirmed the increased volume flow rate of the proposed fan. Then, the rotation speed of the new fan was decreased to match the P-Q curve of the existing fan. It was found that a noise reduction of 1.7 dBA could be achieved using the new fan at the same volume flow rate.

Performance/Noise Optimization of Centrifugal Fan Using Response Surface Method

International Nuclear Information System (INIS)

Shin, Donghui; Cheong, Cheolung; Heo Seung; Kim, Tae-Hoon; Jung, Jiwon

2017-01-01

In this study, centrifugal fan blades used to circulate cold air inside a household refrigerator were optimized to achieve high performance and low noise by using the response surface method, which is frequently employed as an optimization algorithm when multiple independent variables affect one dependent variable. The inlet and outlet blade angles, and the inner radius, were selected as the independent variables. First, the fan blades were optimized to achieve the maximum volume flow rate. Based on this result, a prototype fan blade was manufactured using a 3-D printer. The measured P-Q curves confirmed the increased volume flow rate of the proposed fan. Then, the rotation speed of the new fan was decreased to match the P-Q curve of the existing fan. It was found that a noise reduction of 1.7 dBA could be achieved using the new fan at the same volume flow rate.

A three-term conjugate gradient method under the strong-Wolfe line search

Science.gov (United States)

Khadijah, Wan; Rivaie, Mohd; Mamat, Mustafa

2017-08-01

Recently, numerous studies have been concerned in conjugate gradient methods for solving large-scale unconstrained optimization method. In this paper, a three-term conjugate gradient method is proposed for unconstrained optimization which always satisfies sufficient descent direction and namely as Three-Term Rivaie-Mustafa-Ismail-Leong (TTRMIL). Under standard conditions, TTRMIL method is proved to be globally convergent under strong-Wolfe line search. Finally, numerical results are provided for the purpose of comparison.

Centrifugal compressor fault diagnosis based on qualitative simulation and thermal parameters

Science.gov (United States)

Lu, Yunsong; Wang, Fuli; Jia, Mingxing; Qi, Yuanchen

2016-12-01

This paper concerns fault diagnosis of centrifugal compressor based on thermal parameters. An improved qualitative simulation (QSIM) based fault diagnosis method is proposed to diagnose the faults of centrifugal compressor in a gas-steam combined-cycle power plant (CCPP). The qualitative models under normal and two faulty conditions have been built through the analysis of the principle of centrifugal compressor. To solve the problem of qualitative description of the observations of system variables, a qualitative trend extraction algorithm is applied to extract the trends of the observations. For qualitative states matching, a sliding window based matching strategy which consists of variables operating ranges constraints and qualitative constraints is proposed. The matching results are used to determine which QSIM model is more consistent with the running state of system. The correct diagnosis of two typical faults: seal leakage and valve stuck in the centrifugal compressor has validated the targeted performance of the proposed method, showing the advantages of fault roots containing in thermal parameters.

Centrifugal pump’s impeller optimization using methods of calculation hydrodynamics

Science.gov (United States)

Grigoriev, S.; Mayorov, S.; Polyakov, R.

2017-08-01

The paper features the results of the fluid flow calculation in the channels of varying geometry of the centrifugal pump for the service water in the methanol production chain. Modeling of the flow in ANSYS CFX allowed developing recommendations on adjusting the impeller’s profile, significantly decrease the cavitation wear and increase the lifetime by several times.

Cardiac arrhythmias during aerobatic flight and its simulation on a centrifuge.

Science.gov (United States)

Zawadzka-Bartczak, Ewelina K; Kopka, Lech H

2011-06-01

It is well known that accelerations during centrifuge training and during flight can provoke cardiac arrhythmias. Our study was designed to investigate both the similarities and differences between heart rhythm disturbances during flights and centrifuge tests. There were 40 asymptomatic, healthy pilots who performed two training flights and were also tested in a human centrifuge according to a program of rapid onset rate acceleration (ROR) and of centrifuge simulation of the actual acceleration experienced in flight (Simulation). During the flight and centrifuge tests ECG was monitored with the Holter method. ECG was examined for heart rhythm changes and disturbances. During flights, premature ventricular contractions (PVCs) were found in 25% of the subjects, premature supraventricular contractions (PSVCs) and PVCs with bigeminy in 5%, and pairs of PVCs in 2.5% of subjects. During the centrifuge tests, PVCs were experienced by 45% of the subjects, PSVCs and pairs of PVCs by 7.5%, and PVCs with bigeminy by 2.5%. Sinus bradycardia was observed during flights and centrifuge tests in 7.5% of subjects. Comparative evaluation of electrocardiographic records in military pilots during flights and centrifuge tests demonstrated that: 1) there were no clinically significant arrhythmias recorded; and 2) the frequency and kind of heart rhythm disturbances during aerobatic flight and its simulation on a centrifuge were not identical and did not occur repetitively in the same persons during equal phases of the tests.

Centrifuge separation effect on bacterial indicator reduction in dairy manure.

Science.gov (United States)

Liu, Zong; Carroll, Zachary S; Long, Sharon C; Roa-Espinosa, Aicardo; Runge, Troy

2017-04-15

Centrifugation is a commonly applied separation method for manure processing on large farms to separate solids and nutrients. Pathogen reduction is also an important consideration for managing manure. Appropriate treatment reduces risks from pathogen exposure when manure is used as soil amendments or the processed liquid stream is recycled to flush the barn. This study investigated the effects of centrifugation and polymer addition on bacterial indicator removal from the liquid fraction of manure slurries. Farm samples were taken from a manure centrifuge processing system. There were negligible changes of quantified pathogen indicator concentrations in the low-solids centrate compared to the influent slurry. To study if possible improvements could be made to the system, lab scale experiments were performed investigating a range of g-forces and flocculating polymer addition. The results demonstrated that polymer addition had a negligible effect on the indicator bacteria levels when centrifuged at high g forces. However, the higher g force centrifugation was capable of reducing bacterial indicator levels up to two-log 10 in the liquid stream of the manure, although at speeds higher than typical centrifuge operations currently used for manure processing applications. This study suggests manure centrifuge equipment could be redesigned to provide pathogen reduction to meet emerging issues, such as zoonotic pathogen control. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

The magnetic centrifugal mass filter

International Nuclear Information System (INIS)

Fetterman, Abraham J.; Fisch, Nathaniel J.

2011-01-01

Mass filters using rotating plasmas have been considered for separating nuclear waste and spent nuclear fuel. We propose a new mass filter that utilizes centrifugal and magnetic confinement of ions in a way similar to the asymmetric centrifugal trap. This magnetic centrifugal mass filter is shown to be more proliferation resistant than present technology. This filter is collisional and produces well confined output streams, among other advantages.

The magnetic centrifugal mass filter

Energy Technology Data Exchange (ETDEWEB)

Fetterman, Abraham J.; Fisch, Nathaniel J. [Department of Astrophysical Sciences, Princeton University, Princeton, New Jersey 08540 (United States)

2011-09-15

Mass filters using rotating plasmas have been considered for separating nuclear waste and spent nuclear fuel. We propose a new mass filter that utilizes centrifugal and magnetic confinement of ions in a way similar to the asymmetric centrifugal trap. This magnetic centrifugal mass filter is shown to be more proliferation resistant than present technology. This filter is collisional and produces well confined output streams, among other advantages.

Comparison of preconditioned generalized conjugate gradient methods to two-dimensional neutron and photon transport equation

International Nuclear Information System (INIS)

Chen, G.S.

1997-01-01

We apply and compare the preconditioned generalized conjugate gradient methods to solve the linear system equation that arises in the two-dimensional neutron and photon transport equation in this paper. Several subroutines are developed on the basis of preconditioned generalized conjugate gradient methods for time-independent, two-dimensional neutron and photon transport equation in the transport theory. These generalized conjugate gradient methods are used. TFQMR (transpose free quasi-minimal residual algorithm), CGS (conjuage gradient square algorithm), Bi-CGSTAB (bi-conjugate gradient stabilized algorithm) and QMRCGSTAB (quasi-minimal residual variant of bi-conjugate gradient stabilized algorithm). These sub-routines are connected to computer program DORT. Several problems are tested on a personal computer with Intel Pentium CPU. (author)

Sperm fractions obtained following density gradient centrifugation in human ejaculates show differences in sperm DNA longevity

Directory of Open Access Journals (Sweden)

Jaime Gosálvez

2014-06-01

Conclusion: 1 Unnecessary incubation of spermatozoa prior to artificial insemination or in vitro fertilization, should be avoided, since sperm DNA longevity is significantly reduced after ex vivo sperm handling and 2 Although sperm selection by DCG significantly reduces the baseline levels of SDF of sperm in Fraction 3, sperm DNA longevity in this fraction was ultimately lower following 24 h incubation when compared to sperm recovered from non-centrifuged NSS.

Numerical optimization for separation power of gas centrifuge

International Nuclear Information System (INIS)

Jiang Dongjun; Zeng Shi; Liu Bing

2012-01-01

In order to obtain higher separation power of the gas centrifuge, the code was developed to solve the flow-field of the counter-current to acquire the separation power, which was integrated with the iSight software, so a numerical optimization model for separation power was presented, in which the driver conditions and the geometry parameters of the waste baffle were optimized to get the maximum separation power using the sequential quadratic programming arithmetic, and the 12% higher results was acquired, which shows the feasibility of this method. The results also note that the separation power of gas centrifuge is sensitive to the driver conditions and the structure parameters of the waste baffle, so it is necessary to perform the optimization calculation for the certain gas centrifuge model. (authors)

Plane Couette flow in the presence of a strong centrifugal field

International Nuclear Information System (INIS)

Johnson, E.A.

1982-05-01

The Pomraning problem of plane Couette flow in a strong centrifugal field is studied by several methods: a half-range polynomial expansion of the linearized BGK equation; the Liu-Lees method; and a new matching approximation constructed to give the correct solution in the free-molecule limit. The matching approximation, which appears valid for strong enough centrifugal field, predicts major differences from hydrodynamic behaviour, and suggests ways in which the lack of convergence of one method studied may be corrected. (author)

Design of ideal cascades of gas centrifuges with variable separation factors

International Nuclear Information System (INIS)

Olander, D.R.

1976-01-01

A method of designing ideal cascades in which the separation factor varies with stage number is presented and applied to centrifuges as separating units. The centrifuge is characterized by a performance function, which gives the separative power, optimized with respect to all internal variables, as a function of cut and throughput. For centrifuges with certain types of performance functions, variable-α ideal cascades can provide a product at a lower cost than the conventional ideal cascade in which the separation factor is independent of stage number

Centrifugal compressor shape modification using a proposed inverse design method

International Nuclear Information System (INIS)

Niliahmadabadi, Mahdi; Poursadegh, Farzad

2013-01-01

This paper is concerned with a quasi-3D design method for the radial and axial diffusers of a centrifugal compressor on the meridional plane. The method integrates a novel inverse design algorithm, called ball-spine algorithm (BSA), and a quasi-3D analysis code. The Euler equation is solved on the meridional plane for a numerical domain, of which unknown boundaries (hub and shroud) are iteratively modified under the BSA until a prescribed pressure distribution is reached. In BSA, unknown walls are composed of a set of virtual balls that move freely along specified directions called spines. The difference between target and current pressure distributions causes the flexible boundary to deform at each modification step. In validating the quasi-3D analysis code, a full 3D Navier-Stokes code is used to analyze the existing and designed compressors numerically. Comparison of the quasi-3D analysis results with full 3D analysis results shows viable agreement. The 3D numerical analysis of the current compressor shows a huge total pressure loss on the 90 .deg. bend between the radial and axial diffusers. Geometric modification of the meridional plane causes the efficiency to improve by about 10%.

Centrifugal compressor shape modification using a proposed inverse design method

Energy Technology Data Exchange (ETDEWEB)

Niliahmadabadi, Mahdi [Isfahan University of Technology, Isfahan (Iran, Islamic Republic of); Poursadegh, Farzad [Sharif University of Technology, Tehran (Iran, Islamic Republic of)

2013-03-15

This paper is concerned with a quasi-3D design method for the radial and axial diffusers of a centrifugal compressor on the meridional plane. The method integrates a novel inverse design algorithm, called ball-spine algorithm (BSA), and a quasi-3D analysis code. The Euler equation is solved on the meridional plane for a numerical domain, of which unknown boundaries (hub and shroud) are iteratively modified under the BSA until a prescribed pressure distribution is reached. In BSA, unknown walls are composed of a set of virtual balls that move freely along specified directions called spines. The difference between target and current pressure distributions causes the flexible boundary to deform at each modification step. In validating the quasi-3D analysis code, a full 3D Navier-Stokes code is used to analyze the existing and designed compressors numerically. Comparison of the quasi-3D analysis results with full 3D analysis results shows viable agreement. The 3D numerical analysis of the current compressor shows a huge total pressure loss on the 90 .deg. bend between the radial and axial diffusers. Geometric modification of the meridional plane causes the efficiency to improve by about 10%.

Moving force identification based on modified preconditioned conjugate gradient method

Science.gov (United States)

Chen, Zhen; Chan, Tommy H. T.; Nguyen, Andy

2018-06-01

This paper develops a modified preconditioned conjugate gradient (M-PCG) method for moving force identification (MFI) by improving the conjugate gradient (CG) and preconditioned conjugate gradient (PCG) methods with a modified Gram-Schmidt algorithm. The method aims to obtain more accurate and more efficient identification results from the responses of bridge deck caused by vehicles passing by, which are known to be sensitive to ill-posed problems that exist in the inverse problem. A simply supported beam model with biaxial time-varying forces is used to generate numerical simulations with various analysis scenarios to assess the effectiveness of the method. Evaluation results show that regularization matrix L and number of iterations j are very important influence factors to identification accuracy and noise immunity of M-PCG. Compared with the conventional counterpart SVD embedded in the time domain method (TDM) and the standard form of CG, the M-PCG with proper regularization matrix has many advantages such as better adaptability and more robust to ill-posed problems. More importantly, it is shown that the average optimal numbers of iterations of M-PCG can be reduced by more than 70% compared with PCG and this apparently makes M-PCG a preferred choice for field MFI applications.

A penalized linear and nonlinear combined conjugate gradient method for the reconstruction of fluorescence molecular tomography.

Science.gov (United States)

Shang, Shang; Bai, Jing; Song, Xiaolei; Wang, Hongkai; Lau, Jaclyn

2007-01-01

Conjugate gradient method is verified to be efficient for nonlinear optimization problems of large-dimension data. In this paper, a penalized linear and nonlinear combined conjugate gradient method for the reconstruction of fluorescence molecular tomography (FMT) is presented. The algorithm combines the linear conjugate gradient method and the nonlinear conjugate gradient method together based on a restart strategy, in order to take advantage of the two kinds of conjugate gradient methods and compensate for the disadvantages. A quadratic penalty method is adopted to gain a nonnegative constraint and reduce the illposedness of the problem. Simulation studies show that the presented algorithm is accurate, stable, and fast. It has a better performance than the conventional conjugate gradient-based reconstruction algorithms. It offers an effective approach to reconstruct fluorochrome information for FMT.

Subspace Barzilai-Borwein Gradient Method for Large-Scale Bound Constrained Optimization

International Nuclear Information System (INIS)

Xiao Yunhai; Hu Qingjie

2008-01-01

An active set subspace Barzilai-Borwein gradient algorithm for large-scale bound constrained optimization is proposed. The active sets are estimated by an identification technique. The search direction consists of two parts: some of the components are simply defined; the other components are determined by the Barzilai-Borwein gradient method. In this work, a nonmonotone line search strategy that guarantees global convergence is used. Preliminary numerical results show that the proposed method is promising, and competitive with the well-known method SPG on a subset of bound constrained problems from CUTEr collection

Nanoliter Centrifugal Liquid Dispenser Coupled with Superhydrophobic Microwell Array Chips for High-Throughput Cell Assays

Directory of Open Access Journals (Sweden)

Yuyi Wang

2018-06-01

Full Text Available Microfluidic systems have been regarded as a potential platform for high-throughput screening technology in drug discovery due to their low sample consumption, high integration, and easy operation. The handling of small-volume liquid is an essential operation in microfluidic systems, especially in investigating large-scale combination conditions. Here, we develop a nanoliter centrifugal liquid dispenser (NanoCLD coupled with superhydrophobic microwell array chips for high-throughput cell-based assays in the nanoliter scale. The NanoCLD consists of a plastic stock block with an array of drilled through holes, a reagent microwell array chip (reagent chip, and an alignment bottom assembled together in a fixture. A simple centrifugation at 800 rpm can dispense ~160 nL reagents into microwells in 5 min. The dispensed reagents are then delivered to cells by sandwiching the reagent chip upside down with another microwell array chip (cell chip on which cells are cultured. A gradient of doxorubicin is then dispensed to the cell chip using the NanoCLD for validating the feasibility of performing drug tests on our microchip platform. This novel nanoliter-volume liquid dispensing method is simple, easy to operate, and especially suitable for repeatedly dispensing many different reagents simultaneously to microwells.

Theoretical investigations on plasma centrifuges

International Nuclear Information System (INIS)

Hong, S.H.

1978-01-01

The theoretical analysis of the steady-state dynamics of plasma centrifuges is dealt with to understand the physics of rotating plasmas and their feasibility for isotope separation. The centrifuge systems under consideration employ cylindrical gas discharge chambers with externally-applied axial magnetic fields. The cathode and anode are symmetric about the cylinder axis and arranged in such a way for each system, i.e., (1) two ring electrodes of different radii in the chamber end plates or (2) two ring electrodes embedded in the mantle of the cylinder. They produce converging and/or diverging current density field lines, which intersect the external magnetic field under a nonvanishing angle. The associated Lorentz forces set the plasma, which is produced through an electrical discharge, into rotation around the cylinder axis. Three boundary-value problems for the coupled partial differential equations of the centrifuge fields are formulated, respectively, on the basis of the magnetogasdynamic equations. The electric field, electrostatic potential, current density, induced magnetic field, and velocity distributions are discussed in terms of the Hartmann number, the Hall coefficient, and the magnetic Reynolds number. The plasma centrifuge analyses presented show that the speeds of plasma rotation up to the order of 10 4 m/sec are achievable at typical conditions. The associated centrifugal forces produce a significant spatial isotope separation, which is somewhat reduced in the viscous boundary layers at the centrifuge walls. The speeds of plasma rotation increase with increasing Hartmann number and Hall coefficient. For small Hall coefficient, the induced azimuthal magnetic field does not affect the plasma rotation. For large volumes of rotating isotope mixtures, a multidischarge centrifuge can be constructed by setting up a large number of centrifuge systems in series

2SD numerical study of feed-jet flow in gas centrifuge

International Nuclear Information System (INIS)

Jiang Dongjun; Zeng Shi

2008-01-01

Computational Fluid Dynamics (CFD) method was adopted to simulate the 2D symmetrical feed-jet flow-field in Iguacu gas centrifuge, in order to study the influence of feed-jet to counter-current. The data acquired from calculation were used to modify the feed boundary condition in counter-current calculation, and the stream lines distribution was got considering the effect o f the feed-jet. Finite volume method and 2-order implicit scheme were adopted to solve Navier-Stokes (N-S) equations in cylinder coordinates to simulate the feed-jet flow. Finite difference method was used to solve centrifuge fluid dynamics equations. The result s indicate that the feed-jet flow affects the countercurrent observably, the results of feed-jet flow simulation can be used to modify the conditions to calculate the counter-current in the real centrifuge. (authors)

21 CFR 864.5350 - Microsedimentation centrifuge.

Science.gov (United States)

2010-04-01

... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Microsedimentation centrifuge. 864.5350 Section 864.5350 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... § 864.5350 Microsedimentation centrifuge. (a) Identification. A microsedimentation centrifuge is a...

Spectroscopy of molecules in very high rotational states using an optical centrifuge.

Science.gov (United States)

Yuan, Liwei; Toro, Carlos; Bell, Mack; Mullin, Amy S

2011-01-01

We have developed a high power optical centrifuge for measuring the spectroscopy of molecules in extreme rotational states. The optical centrifuge has a pulse energy that is more than 2 orders of magnitude greater than in earlier instruments. The large pulse energy allows us to drive substantial number densities of molecules to extreme rotational states in order to measure new spectroscopic transitions that are not accessible with traditional methods. Here we demonstrate the use of the optical centrifuge for measuring IR transitions of N2O from states that have been inaccessible until now. In these studies, the optical centrifuge drives N2O molecules into states with J ~ 200 and we use high resolution transient IR probing to measure the appearance of population in states with J = 93-99 that result from collisional cooling of the centrifuged molecules. High resolution Doppler broadened line profile measurements yield information about the rotational and translational energy distributions in the optical centrifuge.

Uranium enrichment by gas centrifuge

International Nuclear Information System (INIS)

Heriot, I.D.

1988-01-01

After recalling the physical principles and the techniques of centrifuge enrichment the report describes the centrifuge enrichment programmes of the various countries concerned and compares this technology with other enrichment technologies like gaseous diffusion, laser, aerodynamic devices and chemical processes. The centrifuge enrichment process is said to be able to replace with advantage the existing enrichment facilities in the short and medium term. Future prospects of the process are also described, like recycled uranium enrichment and economic improvements; research and development needs to achieve the economic prospects are also indicated. Finally the report takes note of the positive aspect of centrifuge enrichment as far as safeguards and nuclear safety are concerned. 27 figs, 113 refs

Centrifugation speed affects light transmission aggregometry.

Science.gov (United States)

Merolla, M; Nardi, M A; Berger, J S

2012-02-01

Light transmission aggregometry (LTA) is considered the gold standard for investigating platelet activity ex vivo. However, LTA protocols are not standardized, and differences in LTA procedure are a potential source of variance in results. Centrifugation speed is an essential component of platelet preparation in LTA, has yet to be standardized, and may affect platelet aggregation results. We sought to investigate the effect of relative centrifugal force (RCF) intensity on LTA results. Ten healthy controls had venous blood drawn and centrifuged at 150, 200, 300, and 500 g for 10 min. Cell counts in whole blood and platelet-rich plasma (PRP) were measured using a hematology analyzer. LTA was performed using 1.0 μm adenosine diphosphate (ADP) and 0.4 μm epinephrine as an agonist. Aggregation (%) was compared at 60, 120, 180, and 300 s and at maximum aggregation. Centrifugation speed was associated with decreasing platelet count (P centrifuge RCF at 60, 120, 180, 300 s and at maximum aggregation (P centrifugation speed in the interpretation of LTA results, supporting the need for standardization of centrifugation RCF in LTA protocols. © 2011 Blackwell Publishing Ltd.

Coalescence and compression in centrifuged emulsions studied with in situ optical microscopy

NARCIS (Netherlands)

Krebs, T.; Ershov, D.S.; Schroën, C.G.P.H.; Boom, R.M.

2013-01-01

We report an experimental method to investigate droplet dynamics in centrifuged emulsions and its application to study droplet compression and coalescence. The experimental setup permits in situ monitoring of an ensemble of droplets in a centrifuged monolayer of monodisperse emulsion droplets using

The potential of centrifugal casting for the production of near net shape uranium parts

International Nuclear Information System (INIS)

Robertson, E.

1993-09-01

This report was written to provide a detailed summary of a literature survey on the near net shape casting process of centrifugal casting. Centrifugal casting is one potential casting method which could satisfy the requirements of the LANL program titled Near Net Shape Casting of Uranium for Reduced Environmental, Safety and Health Impact. In this report, centrifugal casting techniques are reviewed and an assessment of the ability to achieve the near net shape and waste minimization goals of the LANL program by using these techniques is made. Based upon the literature reviewed, it is concluded that if properly modified for operation within a vacuum, vertical or horizontal centrifugation could be used to safely cast uranium for the production of hollow, cylindrical parts. However, for the production of components of geometries other than hollow tubes, vertical centrifugation could be combined with other casting methods such as semi-permanent mold or investment casting

Solving Optimal Control Problem of Monodomain Model Using Hybrid Conjugate Gradient Methods

Directory of Open Access Journals (Sweden)

Kin Wei Ng

2012-01-01

Full Text Available We present the numerical solutions for the PDE-constrained optimization problem arising in cardiac electrophysiology, that is, the optimal control problem of monodomain model. The optimal control problem of monodomain model is a nonlinear optimization problem that is constrained by the monodomain model. The monodomain model consists of a parabolic partial differential equation coupled to a system of nonlinear ordinary differential equations, which has been widely used for simulating cardiac electrical activity. Our control objective is to dampen the excitation wavefront using optimal applied extracellular current. Two hybrid conjugate gradient methods are employed for computing the optimal applied extracellular current, namely, the Hestenes-Stiefel-Dai-Yuan (HS-DY method and the Liu-Storey-Conjugate-Descent (LS-CD method. Our experiment results show that the excitation wavefronts are successfully dampened out when these methods are used. Our experiment results also show that the hybrid conjugate gradient methods are superior to the classical conjugate gradient methods when Armijo line search is used.

A density gradient theory based method for surface tension calculations

DEFF Research Database (Denmark)

Liang, Xiaodong; Michelsen, Michael Locht; Kontogeorgis, Georgios

2016-01-01

The density gradient theory has been becoming a widely used framework for calculating surface tension, within which the same equation of state is used for the interface and bulk phases, because it is a theoretically sound, consistent and computationally affordable approach. Based on the observation...... that the optimal density path from the geometric mean density gradient theory passes the saddle point of the tangent plane distance to the bulk phases, we propose to estimate surface tension with an approximate density path profile that goes through this saddle point. The linear density gradient theory, which...... assumes linearly distributed densities between the two bulk phases, has also been investigated. Numerical problems do not occur with these density path profiles. These two approximation methods together with the full density gradient theory have been used to calculate the surface tension of various...

Theoretical analysis on the refractive-index distribution and bandwidth of gradient-index polymer optical fibers from a centrifugal field.

Science.gov (United States)

Wei, Ming-Hsin; Chen, Wen-Chang

2003-04-20

Theoretical analysis was applied to analyze the refractive-index distribution (RID) and bandwidth (BW) of gradient-index polymer optical fibers (GI POFs) prepared by a centrifugal field process. The RID of the prepared GI POF could be represented by the equation of n(r) = n1[1 - 2delta(r/alpha)g](1/2). The studied material systems were poly(hexafluoroisopropyl 2-fluoroacrylate) (PHFIP 2-FA)/dibutyl phthalate (DBP) and poly(methyl methacrylate) (PMMA)/benzyl benzoate (BEN). The RID and the BW were significantly affected by an essential parameter k, which was related to thematerial properties (density difference and molecular weight) and processing properties (rotating speed, temperature, and radius). As k increased, the characteristic constant of RID, g, decreased to a minimum and then increased sharply, owing to the separation of the polymer and the dopant. On the other hand, the relative refractive-index difference of RID, delta, increased to a steady value after k increased to a certain value. The variation of RID with k resulted in a local minimum of intermodal dispersion, and thus a maximum bandwidth was obtained. The maximum BW of the PHFIP 2-FA/DBP and PMMA/BEN systems at 1550 nm (100-m fiber length and 2-nm spectral width) for the case of k not equal to 0 were 6.7 and 3.2 Gb/s, respectively. The wavelength of light source affects the BW significantly only at k around zero because of the importance of the intramodal dispersion in this case.

[A comparative study of blood culture conventional method vs. a modified lysis/centrifugation technique for the diagnosis of fungemias].

Science.gov (United States)

Santiago, Axel Rodolfo; Hernández, Betsy; Rodríguez, Marina; Romero, Hilda

2004-12-01

The purpose of this work was to compare the efficacy of blood culture conventional method vs. a modified lysis/centrifugation technique. Out of 450 blood specimens received in one year, 100 where chosen for this comparative study: 60 from patients with AIDS, 15 from leukemic patients, ten from febrile neutropenic patients, five from patients with respiratory infections, five from diabetics and five from septicemic patients. The specimens were processed, simultaneously, according to the above mentioned methodologies with daily inspections searching for fungal growth in order to obtain the final identification of the causative agent. The number (40) of isolates recovered was the same using both methods, which included; 18 Candida albicans (45%), ten Candida spp. (25%), ten Histoplasma capsulatum (25%), and two Cryptococcus neoformans (5%). When the fungal growth time was compared by both methods, growth was more rapid when using the modified lysis/centrifugation technique than when using the conventional method. Statistical analysis revealed a significant difference (pcentrifugation technique showed to be more efficacious than the conventional one, and therefore the implementation of this methodology is highly recommended for the isolation of fungi from blood.

Comparison of preconditioned generalized conjugate gradient methods to two-dimensional neutron and photon transport equation

International Nuclear Information System (INIS)

Chen, G.S.; Yang, D.Y.

1998-01-01

We apply and compare the preconditioned generalized conjugate gradient methods to solve the linear system equation that arises in the two-dimensional neutron and photon transport equation in this paper. Several subroutines are developed on the basis of preconditioned generalized conjugate gradient methods for time-independent, two-dimensional neutron and photon transport equation in the transport theory. These generalized conjugate gradient methods are used: TFQMR (transpose free quasi-minimal residual algorithm) CGS (conjugate gradient square algorithm), Bi-CGSTAB (bi-conjugate gradient stabilized algorithm) and QMRCGSTAB (quasi-minimal residual variant of bi-conjugate gradient stabilized algorithm). These subroutines are connected to computer program DORT. Several problems are tested on a personal computer with Intel Pentium CPU. The reasons to choose the generalized conjugate gradient methods are that the methods have better residual (equivalent to error) control procedures in the computation and have better convergent rate. The pointwise incomplete LU factorization ILU, modified pointwise incomplete LU factorization MILU, block incomplete factorization BILU and modified blockwise incomplete LU factorization MBILU are the preconditioning techniques used in the several testing problems. In Bi-CGSTAB, CGS, TFQMR and QMRCGSTAB method, we find that either CGS or Bi-CGSTAB method combined with preconditioner MBILU is the most efficient algorithm in these methods in the several testing problems. The numerical solution of flux by preconditioned CGS and Bi-CGSTAB methods has the same result as those from Cray computer, obtained by either the point successive relaxation method or the line successive relaxation method combined with Gaussian elimination

W-Cu gradient materials - processing, properties and application possibilities

International Nuclear Information System (INIS)

Joensson, M.; Kieback, B.

2001-01-01

The functionally graded material (FGM) of tungsten with its high thermal and mechanical resistance and copper with its very high thermal and electrical conductivity and ductility expands the application fields of this material in the direction of extreme demands such as plasma facing components in fusion reactors. The PM-production of W-Cu-gradients recommends itself because of the possibility to form the gradient by the mixing of powder components, but is also demanding because of the differences in their sintering behavior and thermal expansions. W-Gu-gradient samples of different concentration profiles have been formed in layers by powder stacking in a die and continuously by centrifugal powder forming. The consolidation routes were determined by the concentration areas of the gradients and encompass liquid phase sintering, pressure assisted solid phase sintering and the application of coated Tungsten powder and sintering additives. The microstructure and the concentration profiles of the samples have been investigated metaliographically and by EDX. The influence of processing and the gradient profile of the properties have been characterized by TRS and the investigation of residual thermal stresses by neutron diffraction. (author)

Differential centrifugation of leucocytes and platelets applied to 111In-leucocyte labelling

International Nuclear Information System (INIS)

Mountford, P.J.

1986-01-01

Platelet contamination of 111 In-leucocytes can be minimized by centrifugation of leucocyte-rich plasma before labelling. The differential recovery of leucocytes and platelets from centrifugation depends on various biological and physical factors. Experimental measurements have been made of the effect of some of these factors. The ratio of leucocyte-to-platelet recovery was increased by using more than one centrifugation, and by using a low relative centrifugal force (RCF). To extend the study, a method of calculating cell recovery from centrifugation was developed, based on the experimental results. Calculations indicated that this ratio was at a maximum at around 85g RCF, and was least affected by changes in the suspension medium viscosity. At 85g, leucocyte recovery was incomplete, but calculations indicated that there was a preferential recovery of granulocytes which was subsequently verified by experiment. (author)

Hydraulic and separation characteristics of an industrial gas centrifuge calculated with neural networks

Science.gov (United States)

Butov, Vladimir; Timchenko, Sergey; Ushakov, Ivan; Golovkov, Nikita; Poberezhnikov, Andrey

2018-03-01

Single gas centrifuge (GC) is generally used for the separation of binary mixtures of isotopes. Processes taking place within the centrifuge are complex and non-linear. Their characteristics can change over time with long-term operation due to wear of the main structural elements of the GC construction. The paper is devoted to the determination of basic operation parameters of the centrifuge with the help of neural networks. We have developed a method for determining the parameters of the industrial GC operation by processing statistical data. In this work, we have constructed a neural network that is capable of determining the main hydraulic and separation characteristics of the gas centrifuge, depending on the geometric dimensions of the gas centrifuge, load value, and rotor speed.

An optimized target-field method for MRI transverse biplanar gradient coil design

International Nuclear Information System (INIS)

Zhang, Rui; Xu, Jing; Huang, Kefu; Zhang, Jue; Fang, Jing; Fu, Youyi; Li, Yangjing

2011-01-01

Gradient coils are essential components of magnetic resonance imaging (MRI) systems. In this paper, we present an optimized target-field method for designing a transverse biplanar gradient coil with high linearity, low inductance and small resistance, which can well satisfy the requirements of permanent-magnet MRI systems. In this new method, the current density is expressed by trigonometric basis functions with unknown coefficients in polar coordinates. Following the standard procedures, we construct an objective function with respect to the total square errors of the magnetic field at all target-field points with the penalty items associated with the stored magnetic energy and the dissipated power. By adjusting the two penalty factors and minimizing the objective function, the appropriate coefficients of the current density are determined. Applying the stream function method to the current density, the specific winding patterns on the planes can be obtained. A novel biplanar gradient coil has been designed using this method to operate in a permanent-magnet MRI system. In order to verify the validity of the proposed approach, the gradient magnetic field generated by the resulted current density has been calculated via the Biot–Savart law. The results have demonstrated the effectiveness and advantage of this proposed method

Problem of unstable pivots in the incomplete LU-conjugate gradient method

International Nuclear Information System (INIS)

Kershaw, D.S.

1978-01-01

Incomplete LU and incomplete-Cholesky conjugate gradient methods are becoming widely used in both laser and magnetic fusion research. In my original presentation of these methods, the problem of what to do if a pivot [L/sub ii/U/sub ii/) becomes very small or zero was raised and only partially answered by the suggestion that it be arbitrarily set to some non-zero value. In what follows it will be shown precisely how small the pivot can become before it must be fixed and precisely what value it should be set to in order to minimize the error in LU. Numerical examples will be given to show that not only does this prescription improve incomplete LU-conjugate gradient methods , but exact LU decomposition carried out with this prescription for handling small pivots and followed by a few linear or conjugate gradient iterations can be much faster than the permutations of rows and columns usually employed to circumvent small pivot problems

Progress in ultra-centrifuge enrichment technology

International Nuclear Information System (INIS)

Paul Dawson

2006-01-01

Urenco have undertaken a continuous development programme in centrifuge technology for over 35 years. This has seen development from sub-critical machines in the mid 1970's through to the company's world leading TC12 supercritical centrifuge, which has been deployed on a large-scale basis over the last decade. The latest centrifuge to emerge from this programme is Urenco's sixth generation centrifuge, the TC21, which will be commercially deployed from mid-2007 onwards. In recent times Urenco has vested its centrifuge technology in Enrichment Technology Company (ETC) as a vehicle to enable the use of this advanced technology by other operators for commercial purposes. This paper reviews why Urenco and ETC believe this technology represents the best choice for creating new global commercial enrichment capacity and its future development prospects. (author)

A vibration model for centrifugal contactors

International Nuclear Information System (INIS)

Leonard, R.A.; Wasserman, M.O.; Wygmans, D.G.

1992-11-01

Using the transfer matrix method, we created the Excel worksheet ''Beam'' for analyzing vibrations in centrifugal contactors. With this worksheet, a user can calculate the first natural frequency of the motor/rotor system for a centrifugal contactor. We determined a typical value for the bearing stiffness (k B ) of a motor after measuring the k B value for three different motors. The k B value is an important parameter in this model, but it is not normally available for motors. The assumptions that we made in creating the Beam worksheet were verified by comparing the calculated results with those from a VAX computer program, BEAM IV. The Beam worksheet was applied to several contactor designs for which we have experimental data and found to work well

Simulation model for centrifugal pump in flow networks based on internal characteristics

International Nuclear Information System (INIS)

Sun, Ji-Lin; Xue, Ruo-Jun; Peng, Min-Jun

2018-01-01

For the simulation of centrifugal pump in flow network system, in general three approaches can be used, the fitting model, the numerical method and the internal characteristics model. The fitting model is simple and rapid thus widely used. The numerical method can provide more detailed information in comparison with the fitting model, but increases implementation complexity and computational cost. In real-time simulations of flow networks, to simulate the condition out of the rated condition, especially for the volume flow rate, which the accuracy of fitting model is incredible, a new method for simulating centrifugal pumps was proposed in this research. The method based on the theory head and hydraulic loss in centrifugal pumps, and cavitation is also to be considered. The simulation results are verified with experimental benchmark data from an actual pump. The comparison confirms that the proposed method could fit the flow-head curves well, and the responses of main parameters in dynamic-state operations are consistent with theoretical analyses.

Development and industrial application of gas centrifuges to uranium enrichment in the USSR

International Nuclear Information System (INIS)

Abbakumov, E.I.; Bazhenov, V.A.; Verbin, Yu.V.

1989-01-01

Review of state and studies in the field of gaseous diffusion technology and centrifugal method of uranium enrichment in the USSR is given. Domestic industrial gas centrifuges, forming to-day the main part of separation capacities in the USSR, are noted for low specific energy consumption and high reliability. Centrifugal technology in the USSR is applied both to uranium enrichment (including one for export) and to separation of isotopes of other chemical elements

Empirical Design Considerations for Industrial Centrifugal Compressors

Directory of Open Access Journals (Sweden)

Cheng Xu

2012-01-01

Full Text Available Computational Fluid Dynamics (CFD has been extensively used in centrifugal compressor design. CFD provides further optimisation opportunities for the compressor design rather than designing the centrifugal compressor. The experience-based design process still plays an important role for new compressor developments. The wide variety of design subjects represents a very complex design world for centrifugal compressor designers. Therefore, some basic information for centrifugal design is still very important. The impeller is the key part of the centrifugal stage. Designing a highly efficiency impeller with a wide operation range can ensure overall stage design success. This paper provides some empirical information for designing industrial centrifugal compressors with a focus on the impeller. A ported shroud compressor basic design guideline is also discussed for improving the compressor range.

Direct method of design and stress analysis of rotating disks with temperature gradient

Science.gov (United States)

Manson, S S

1950-01-01

A method is presented for the determination of the contour of disks, typified by those of aircraft gas turbines, to incorporate arbitrary elastic-stress distributions resulting from either centrifugal or combined centrifugal and thermal effects. The specified stress may be radial, tangential, or any combination of the two. Use is made of the finite-difference approach in solving the stress equations, the amount of computation necessary in the evolution of a design being greatly reduced by the judicious selection of point stations by the aid of a design chart. Use of the charts and of a preselected schedule of point stations is also applied to the direct problem of finding the elastic and plastic stress distribution in disks of a given design, thereby effecting a great reduction in the amount of calculation. Illustrative examples are presented to show computational procedures in the determination of a new design and in analyzing an existing design for elastic stress and for stresses resulting from plastic flow.

A Breast Cell Atlas: Organelle analysis of the MDA-MB-231 cell line by density-gradient fractionation using isotopic marking and label-free analysis

Directory of Open Access Journals (Sweden)

Marianne Sandin

2015-09-01

Full Text Available Protein translocation between organelles in the cell is an important process that regulates many cellular functions. However, organelles can rarely be isolated to purity so several methods have been developed to analyse the fractions obtained by density gradient centrifugation. We present an analysis of the distribution of proteins amongst organelles in the human breast cell line, MDA-MB-231 using two approaches: an isotopic labelling and a label-free approach.

Centrifuge Health Monitoring of the 50gTon beam centrifuge at the University of Sheffield

OpenAIRE

Cox, C.M.; Black, J.A.; Hakhamanshi, M.; Baker, N.

2016-01-01

In order to fully understand scientific test data it is crucial that we first understand the back-ground centrifuge operational environment and its variation with time and centrifugal acceleration. For exam-ple, changes in ambient air temperature or relative humidity in the centrifuge chamber during operation can have a significant impact on the evaporation levels of water from the surface of a clay model. It is vital to un-derstand these temporal changes in order to mitigate drying out of th...

Perceived radial translation during centrifugation

NARCIS (Netherlands)

Bos, J.E.; Correia Grácio, B.J.

2015-01-01

BACKGROUND: Linear acceleration generally gives rise to translation perception. Centripetal acceleration during centrifugation, however, has never been reported giving rise to a radial, inward translation perception. OBJECTIVE: To study whether centrifugation can induce a radial translation

Surge control of the electrically driven centrifugal compressor

NARCIS (Netherlands)

Boinov, K.O.; Lomonova, E.A.; Vandenput, A.J.A.; Tyagounov, A.

2006-01-01

This paper presents a method of the energy efficiency and the operational performance improvement of the electrically driven air compression system. The key innovation of the proposed method-the active surge suppression of the centrifugal compressor by means of the speed control of the electrical

Momentum-weighted conjugate gradient descent algorithm for gradient coil optimization.

Science.gov (United States)

Lu, Hanbing; Jesmanowicz, Andrzej; Li, Shi-Jiang; Hyde, James S

2004-01-01

MRI gradient coil design is a type of nonlinear constrained optimization. A practical problem in transverse gradient coil design using the conjugate gradient descent (CGD) method is that wire elements move at different rates along orthogonal directions (r, phi, z), and tend to cross, breaking the constraints. A momentum-weighted conjugate gradient descent (MW-CGD) method is presented to overcome this problem. This method takes advantage of the efficiency of the CGD method combined with momentum weighting, which is also an intrinsic property of the Levenberg-Marquardt algorithm, to adjust step sizes along the three orthogonal directions. A water-cooled, 12.8 cm inner diameter, three axis torque-balanced gradient coil for rat imaging was developed based on this method, with an efficiency of 2.13, 2.08, and 4.12 mT.m(-1).A(-1) along X, Y, and Z, respectively. Experimental data demonstrate that this method can improve efficiency by 40% and field uniformity by 27%. This method has also been applied to the design of a gradient coil for the human brain, employing remote current return paths. The benefits of this design include improved gradient field uniformity and efficiency, with a shorter length than gradient coil designs using coaxial return paths. Copyright 2003 Wiley-Liss, Inc.

Effects of centrifugal modification of magnetohydrodynamic equilibrium on resistive wall mode stability

International Nuclear Information System (INIS)

Shiraishi, J.; Aiba, N.; Miyato, N.; Yagi, M.

2014-01-01

Toroidal rotation effects are self-consistently taken into account not only in the linear magnetohydrodynamic (MHD) stability analysis but also in the equilibrium calculation. The MHD equilibrium computation is affected by centrifugal force due to the toroidal rotation. To study the toroidal rotation effects on resistive wall modes (RWMs), a new code has been developed. The RWMaC modules, which solve the electromagnetic dynamics in vacuum and the resistive wall, have been implemented in the MINERVA code, which solves the Frieman–Rotenberg equation that describes the linear ideal MHD dynamics in a rotating plasma. It is shown that modification of MHD equilibrium by the centrifugal force significantly reduces growth rates of RWMs with fast rotation in the order of M 2  = 0.1 where M is the Mach number. Moreover, it can open a stable window which does not exist under the assumption that the rotation affects only the linear dynamics. The rotation modifies the equilibrium pressure gradient and current density profiles, which results in the change of potential energy including rotational effects. (paper)

Application of the gradient method to Hartree-Fock-Bogoliubov theory

International Nuclear Information System (INIS)

Robledo, L. M.; Bertsch, G. F.

2011-01-01

A computer code is presented for solving the equations of the Hartree-Fock-Bogoliubov (HFB) theory by the gradient method, motivated by the need for efficient and robust codes to calculate the configurations required by extensions of the HFB theory, such as the generator coordinate method. The code is organized with a separation between the parts that are specific to the details of the Hamiltonian and the parts that are generic to the gradient method. This permits total flexibility in choosing the symmetries to be imposed on the HFB solutions. The code solves for both even and odd particle-number ground states, with the choice determined by the input data stream. Application is made to the nuclei in the sd shell using the universal sd-shell interaction B (USDB) shell-model Hamiltonian.

Enriched surface acidity for surfactant-free suspensions of carboxylated carbon nanotubes purified by centrifugation

Directory of Open Access Journals (Sweden)

Elizabeth I. Braun

2016-06-01

Full Text Available It is well known that surfactant-suspended carbon nanotube (CNT samples can be purified by centrifugation to decrease agglomerates and increase individually-dispersed CNTs. However, centrifugation is not always part of protocols to prepare CNT samples used in biomedical applications. Herein, using carboxylated multi-walled CNTs (cMWCNTs suspended in water without a surfactant, we developed a Boehm titrimetric method for the analysis of centrifuged cMWCNT suspensions and used it to show that the surface acidity of oxidized carbon materials in aqueous cMWCNT suspensions was enriched by ∼40% by a single low-speed centrifugation step. This significant difference in surface acidity between un-centrifuged and centrifuged cMWCNT suspensions has not been previously appreciated and is important because the degree of surface acidity is known to affect the interactions of cMWCNTs with biological systems.

Accelerated gradient methods for constrained image deblurring

International Nuclear Information System (INIS)

Bonettini, S; Zanella, R; Zanni, L; Bertero, M

2008-01-01

In this paper we propose a special gradient projection method for the image deblurring problem, in the framework of the maximum likelihood approach. We present the method in a very general form and we give convergence results under standard assumptions. Then we consider the deblurring problem and the generality of the proposed algorithm allows us to add a energy conservation constraint to the maximum likelihood problem. In order to improve the convergence rate, we devise appropriate scaling strategies and steplength updating rules, especially designed for this application. The effectiveness of the method is evaluated by means of a computational study on astronomical images corrupted by Poisson noise. Comparisons with standard methods for image restoration, such as the expectation maximization algorithm, are also reported.

The Advanced Gas Centrifuge program

International Nuclear Information System (INIS)

Riepe, R.

1984-01-01

Although the gas centrifuge process for uranium enrichment is often referred to as a ''new technology,'' it has been under development for approximately 25 years to bring it to its current state of deployment. Centrifuges are now being installed in a new gas centrifuge enrichment plant (GCEP) at Portsmouth, Ohio. The objective of this new plant was to provide additional U.S. uranium enrichment capacity at a production cost comparable to the U.S. diffusion process but requiring much less power per separative work unit (SWU) produced. The current, commercial scale centrifuge technology being installed meets that objective. The objective for new U.S. enrichment capacity has changed. The objective is not to provide more SWUs but to provide cheaper SWUs. The objective is to make the U.S. uranium enrichment enterprise competitive on the international market. Where the U.S. at one time supplied virtually all of the free world SWU demand, the U.S. market share has now dropped to approximately 35% of the foreign free world market. The Advanced Gas Centrifuge (AGC) program provides an avenue for making the U.S. the economically attractive, reliable enrichment supplier

Application of conjugate gradient method to Commix-1B three-dimensional momentum equation

International Nuclear Information System (INIS)

King, J.B.; Domanus, H.

1987-01-01

Conjugate gradient method which is a special case of the variational method was implemented in the momentum section of the COMMIX-1B thermal hydraulics code. The comparisons between this method and the conventional iterative method of Successive Over Relation (S.O.R.) were made. Using COMMIX-1B, three steady state problems were analyzed. These problems were flow distribution in a scaled model of the Clinch River Fast Breeder Reactor outlet plenum, flow of coolant in the cold leg and downcomer of a PWR and isothermal air flow through a partially blocked pipe. It was found that if the conjugate gradient method is used, the execution time required to solve the resulting COMMIX-1B system of equations can be reduced by a factor of about 2 for the first two problems. For the isothermal air flow problem, the conjugate gradient method did not improve the execution time

Effects of different centrifugation conditions on clinical chemistry and Immunology test results

Directory of Open Access Journals (Sweden)

Nesic Predrag

2011-05-01

Full Text Available Abstract Background The effect of centrifugation time of heparinized blood samples on clinical chemistry and immunology results has rarely been studied. WHO guideline proposed a 15 min centrifugation time without citing any scientific publications. The centrifugation time has a considerable impact on the turn-around-time. Methods We investigated 74 parameters in samples from 44 patients on a Roche Cobas 6000 system, to see whether there was a statistical significant difference in the test results among specimens centrifuged at 2180 g for 15 min, at 2180 g for 10 min or at 1870 g for 7 min, respectively. Two tubes with different plasma separators (both Greiner Bio-One were used for each centrifugation condition. Statistical comparisons were made by Deming fit. Results Tubes with different separators showed identical results in all parameters. Likewise, excellent correlations were found among tubes to which different centrifugation conditions were applied. Fifty percent of the slopes lay between 0.99 and 1.01. Only 3.6 percent of the statistical tests results fell outside the significance level of p Conclusion A centrifugation time of either 7 or 10 min provided identical test results compared to the time of 15 min as proposed by WHO under the conditions used in our study.

Conjugate Gradient like methods and their application to fixed source neutron diffusion problems

International Nuclear Information System (INIS)

Suetomi, Eiichi; Sekimoto, Hiroshi

1989-01-01

This paper presents a number of fast iterative methods for solving systems of linear equations appearing in fixed source problems for neutron diffusion. We employed the conjugate gradient and conjugate residual methods. In order to accelerate the conjugate residual method, we proposed the conjugate residual squared method by transforming the residual polynomial of the conjugate residual method. Since the convergence of these methods depends on the spectrum of coefficient matrix, we employed the incomplete Choleski (IC) factorization and the modified IC (MIC) factorization as preconditioners. These methods were applied to some neutron diffusion problems and compared with the successive overrelaxation (SOR) method. The results of these numerical experiments showed superior convergence characteristics of the conjugate gradient like method with MIC factorization to the SOR method, especially for a problem involving void region. The CPU time of the MICCG, MICCR and MICCRS methods showed no great difference. In order to vectorize the conjugate gradient like methods based on (M)IC factorization, the hyperplane method was used and implemented on the vector computers, the HITAC S-820/80 and ETA10-E (one processor mode). Significant decrease of the CPU times was observed on the S-820/80. Since the scaled conjugate gradient (SCG) method can be vectorized with no manipulation, it was also compared with the above methods. It turned out the SCG method was the fastest with respect to the CPU times on the ETA10-E. These results suggest that one should implement suitable algorithm for different vector computers. (author)

Valve for gas centrifuges

Science.gov (United States)

Hahs, Charles A.; Burbage, Charles H.

1984-01-01

The invention is a pneumatically operated valve assembly for simultaneously (1) closing gas-transfer lines connected to a gas centrifuge or the like and (2) establishing a recycle path between two of the lines so closed. The valve assembly is especially designed to be compact, fast-acting, reliable, and comparatively inexpensive. It provides large reductions in capital costs for gas-centrifuge cascades.

Ionospheric forecasting model using fuzzy logic-based gradient descent method

Directory of Open Access Journals (Sweden)

D. Venkata Ratnam

2017-09-01

Full Text Available Space weather phenomena cause satellite to ground or satellite to aircraft transmission outages over the VHF to L-band frequency range, particularly in the low latitude region. Global Positioning System (GPS is primarily susceptible to this form of space weather. Faulty GPS signals are attributed to ionospheric error, which is a function of Total Electron Content (TEC. Importantly, precise forecasts of space weather conditions and appropriate hazard observant cautions required for ionospheric space weather observations are limited. In this paper, a fuzzy logic-based gradient descent method has been proposed to forecast the ionospheric TEC values. In this technique, membership functions have been tuned based on the gradient descent estimated values. The proposed algorithm has been tested with the TEC data of two geomagnetic storms in the low latitude station of KL University, Guntur, India (16.44°N, 80.62°E. It has been found that the gradient descent method performs well and the predicted TEC values are close to the original TEC measurements.

A vibration model for centrifugal contactors

Energy Technology Data Exchange (ETDEWEB)

Leonard, R.A.; Wasserman, M.O.; Wygmans, D.G.

1992-11-01

Using the transfer matrix method, we created the Excel worksheet ``Beam`` for analyzing vibrations in centrifugal contactors. With this worksheet, a user can calculate the first natural frequency of the motor/rotor system for a centrifugal contactor. We determined a typical value for the bearing stiffness (k{sub B}) of a motor after measuring the k{sub B} value for three different motors. The k{sub B} value is an important parameter in this model, but it is not normally available for motors. The assumptions that we made in creating the Beam worksheet were verified by comparing the calculated results with those from a VAX computer program, BEAM IV. The Beam worksheet was applied to several contactor designs for which we have experimental data and found to work well.

Centrifuge facilities at Technical University of Denmark

DEFF Research Database (Denmark)

Leth, Caspar Thrane; Krogsbøll, Anette Susanne; Hededal, Ole

2008-01-01

The geotechnical group at the Danish Technical University (DTU) operates a geotechnical beam centrifuge. The centrifuge was build in 1976 and has been upgraded through the years, latest with onboard data and control systems. The centrifuge concept involves an increased gravity field in which...... the physical model is placed and tested. The capabilities of the centrifuge at DTU makes it possible to obtain a scale factor of 75-85 in the tests which equals a soil volume in prototype scale of ø40m and a depth of 36 m. The centrifuge facilities at DTU have through the years been used for testing various...... geotechnical issues, such as suction anchors, tension piles in clay, active earth pressures on sheet piles and group effects for lateral loaded piles. The paper describes physical modelling in general, the centrifuge, present setups and shows samples of obtained results....

Analysis of Vaneless Diffuser Stall Instability in a Centrifugal Compressor

Directory of Open Access Journals (Sweden)

Elias Sundström

2017-11-01

Full Text Available Numerical simulations based on the large eddy simulation approach were conducted with the aim to explore vaneless diffuser rotating stall instability in a centrifugal compressor. The effect of the impeller blade passage was included as an inlet boundary condition with sufficiently low flow angle relative to the tangent to provoke the instability and cause circulation in the diffuser core flow. Flow quantities, velocity and pressure, were extracted to accumulate statistics for calculating mean velocity and mean Reynolds stresses in the wall-to-wall direction. The paper focuses on the assessment of the complex response of the system to the velocity perturbations imposed, the resulting pressure gradient and flow curvature effects.

Ultrasonic wave propagation through aberrating layers: experimental verification of the conjugate gradient Rayleigh method

NARCIS (Netherlands)

Ledoux, L.A.F.; Berkhoff, Arthur P.; Thijssen, J.M.

The Conjugate Gradient Rayleigh method for the calculation of acoustic reflection and transmission at a rough interface between two media was experimentally verified. The method is based on a continuous version of the conjugate gradient technique and plane-wave expansions. We measured the beam

Necessary and sufficient conditions for the existence of a conjugate gradient method

International Nuclear Information System (INIS)

Faber, V.; Manteuffel, T.

1984-01-01

The authors characterize the class CG(s) of matrices A for which the linear system Ax = b can be solved by an s-term conjugate gradient method. The authors show that, except for a few anomalies, the class CG(s) consists of matrices A for which conjugate gradient methods are already known. These matrices are the Hermitian matrices, A* = A, and the matrices of the form A = e/sup i theta/(dI + B), with B* = -B. 7 references

Centrifugal pumps and allied machinery

CERN Document Server

Anderson, HH

1994-01-01

This book will be of vital interest to all engineers and designers concerned with centrifugal pumps and turbines. Including statistical information derived from 20000 pumps and 700 turbines with capacities of 5gpm to 5000000gpm, this book offers the widest range and scope of information currently available. Statistical analyses suggest practical methods of increasing pump performance and provide valuable data for new design aspects.

Some engineering considerations when designing centrifuge enrichment plants

International Nuclear Information System (INIS)

Edwards, T.T.

1982-01-01

A review is given of the three main areas where flexibility is needed in the design of centrifuge enrichment plants. These are: the need to cope with market requirements, the limitations imposed by currently available centrifuges and ever advancing centrifuge technology. Details of BNFL's experience with centrifuge enrichment at Capenhurst are presented. (U.K.)

Flow behaviour and robustness of non-segregating tailings made from filtered/centrifuged MFT

Energy Technology Data Exchange (ETDEWEB)

Nik, R.M.; Sego, D.C.; Morgenstern, N.R. [Alberta Univ., Edmonton, AB (Canada). Geotechnical Center

2010-07-01

This PowerPoint presentation described an experimental study of a centrifugal dewatering filtration process for mature fine tailings (MFT). Various MFT samples from different oil sands operations were pumped into a filtering centrifuge that produced cake, thin tailings, and filtrate. The MFT was then transformed into non-segregated tailings (NST) and composite tailings (CT). The depositional characteristics of the CT-NST samples were evaluated in a series of flume tests. Flow profiles were presented for various samples. Vane shear tests were also conducted. The yield stress of each sample was compared with its flow duration and solids content. The results of the experimental tests demonstrated that the centrifugal filtration process can be considered as the initial stage of a multi-stage tailings management plan. The filtering centrifuge method can be used to produce robust CTs with higher solids content. Use of the method can decrease the amount of coagulants or flocculants required for further treatment. tabs., figs.

A centrifuge-less plasma separation method from whole blood anticoagulated with EDTA-2K for the use of clinical laboratory tests.

Science.gov (United States)

Tatsumi, N; Tsuda, I; Fukumori, T; Hino, M; Takubo, T; Kondo, H

2001-12-01

In the modern medical laboratory system, simple and rapid processing of specimens are required. In the current system with the transportation line, its centrifugation part would disturb smooth flow of the testing because it needs much time for the centrifugation. To solve the problems, a serum separation method was tried for the whole blood specimen using poly-L-lysine, concanavalin A and phyto-hemoagglutinin. Ploy-L-lysine with molecular weight 130,000 to 210,000 in a final concentration of 0.1% could accelerate blood sedimentation, although its supernatant contaminated platelets. Concanavalin and phytohemoagulutinin could accelerate the sedimentation and obtained plasma, but the method could yield enough amount of supernatant by 1 hour standing. As the purpose of this study is to develop a centrifugeless method, a sieve method using a steel mesh and a magnet was applied to the mixture of EDTA blood, red-cell adhesives and thrombin. The method was unique to separate plasma, but the yield was not so high and chemistry data were not fitted with serum data in some of tests. Thus, the trial would be a new technology, but it was judged that some further improvement will be needed technically.

SEPARATION OF X-BEARING BOVINE SPERM BY CENTRIFUGATION IN CONTINUOUS PERCOLL AND OPTIPREP DENSITY GRADIENT: EFFECT IN SPERM VIABILITY AND IN VITRO EMBRYO PRODUCTION SEPARAÇÃO DE ESPERMATOZOIDES PORTADORES DO CROMOSSOMO X BOVINO POR CENTRIFUGAÇÃO EM GRADIENTE DE DENSIDADE CONTÍNUO DE PERCOLL E OPTIPREP: EFEITO SOBRE A VIABILIDADE ESPERMÁTICA E NA PRODUÇÃO IN VITRO DE EMBRIÕES

Directory of Open Access Journals (Sweden)

Aline Costa Lucio

2009-07-01

Full Text Available

The aim of this study was to separate X-bearing bovine sperm by continuous Percoll and OptiPrep density gradients and to validate the sexing of resultant in vitro produced embryos by Polimerase Chain Reaction (PCR. Frozen/thawed sperm was layered on density gradients which were previously prepared in polystyrene tubes, 24 h before procedures and maintained at 4 °C. The tubes were centrifuged at 500 x g for 15 min at 22 °C. Supernatants were gently aspirated and the sperm recovered from the bottom of the tubes. Viability and integrity of sperm were evaluated by Trypan Blue/Giemsa stain. Cleavage and blastocyst rates were determined by in vitro production of embryos and PCR was performed for identification of the embryos’ genetic sex. No damage in viability and acrossomal integrity and in cleavage and blastocyst rates was found in the Percoll and OptiPrep treatment compared to the non-centrifuged group (P>0.05. The percentage of female embryos in the Percoll and OptiPrep group was 63.0 and 47.6%, respectively. The female embryos in control group were 48.7%. A sexual deviation in the Percoll density gradient was achieved without reduction of sperm viability and in vitro production rates.

KEY WORDS: Bovine, centrifugation, in vitro production of embryos, PCR, X-bearing sperm.

A new smoothing modified three-term conjugate gradient method for [Formula: see text]-norm minimization problem.

Science.gov (United States)

Du, Shouqiang; Chen, Miao

2018-01-01

We consider a kind of nonsmooth optimization problems with [Formula: see text]-norm minimization, which has many applications in compressed sensing, signal reconstruction, and the related engineering problems. Using smoothing approximate techniques, this kind of nonsmooth optimization problem can be transformed into a general unconstrained optimization problem, which can be solved by the proposed smoothing modified three-term conjugate gradient method. The smoothing modified three-term conjugate gradient method is based on Polak-Ribière-Polyak conjugate gradient method. For the Polak-Ribière-Polyak conjugate gradient method has good numerical properties, the proposed method possesses the sufficient descent property without any line searches, and it is also proved to be globally convergent. Finally, the numerical experiments show the efficiency of the proposed method.

Unsteady flow measurements in centrifugal compressors

International Nuclear Information System (INIS)

Bammert, K.; Mobarak, A.; Rautenberg, M.

1976-01-01

Centrifugal compressors and blowers are often used for recycling the coolant gas in gas-cooled reactors. To achieve the required high pressure ratios, highly loaded centrifugal compressors are built. The paper deals with unsteady flow measurements on highly loaded centrifugal impellers. Measurements of the approaching flow have been done with hot wires. The method of measurement enabled us to get the velocity distribution across the pitch ahead of the inducer. The static pressure signals along the shroud line has been discussed on the basis of some theoretical considerations. Accordingly the form of flow in the impeller and the wave flow or separation zones in the impeller can now be better interpreted. The importance of the unsteady nature of the relative flow, especially at impeller exit, is clearly demonstrated. Measurements with high responsive total pressure probes in the vicinity of impeller exit and the subsequent calculations have shown, that the instantaneous energy transfer at a certain point after the impeller may differ by more than 30% from the Euler work. Lastly, unsteady pressure measurements along the shroud line have been performed during surge and rotating stall. The surge signal have been analyzed in more detail and the mechanism of flow rupture and pressure recovery during a surge cycle is thoroughly discussed. (orig.) [de

Comparison of DNA double-strand break rejoining as measured by pulsed field gel electrophoresis, neutral sucrose gradient centrifugation and non-unwinding filter elution in irradiated plateau-phase CHO cells

International Nuclear Information System (INIS)

Iliakis, G.; Metzger, L.; Pantelias, G.

1991-01-01

The initial (up to 30 min) rate of DNA double-strand break (dsb) rejoining was measured in irradiated plateau-phase CHO cells, in a set of parallel experiments using the same cell suspension, by means of non-unwinding filter elution, neutral sucrose gradient centrifugation, and two pulsed-field gel electrophoresis assays: asymmetric field inversion gel electrophoresis (AFIGE) and clamped homogeneous electric field (CHEF) gel electrophoresis. The rate of DNA dsb rejoining was compared to the rate of rejoining of chromatin breaks measured, also in the same cell population, using the technique of premature chromosome condensation (PCC). Two radiation exposures, 25 Gy and/or 50 Gy, were used and applied to the individual parts of the experiments according to the sensitivity of the assay under investigation. The results suggest all major techniques currently used for assaying rejoining of DNA dsb give similar results, and indicate that more information is required before a direct correlation between rejoining of DNA dsb and rejoining of chromatin breaks can be established. (author)

Numerical analysis of the internal flow field in screw centrifugal blood pump based on CFD

Science.gov (United States)

Han, W.; Han, B. X.; Y Wang, H.; Shen, Z. J.

2013-12-01

As to the impeller blood pump, the high speed of the impeller, the local high shear force of the flow field and the flow dead region are the main reasons for blood damage. The screw centrifugal pump can effectively alleviate the problems of the high speed and the high shear stress for the impeller. The softness and non-destructiveness during the transfer process can effectively reduce the extent of the damage. By using CFD software, the characteristics of internal flow are analyzed in the screw centrifugal pump by exploring the distribution rules of the velocity, pressure and shear deformation rate of the blood when it flows through the impeller and the destructive effects of spiral blades on blood. The results show that: the design of magnetic levitation solves the sealing problems; the design of regurgitation holes solves the problem of the flow dead zone; the magnetic levitated microcirculation screw centrifugal pump can effectively avoid the vortex, turbulence and high shear forces generated while the blood is flowing through the pump. Since the distribution rules in the velocity field, pressure field and shear deformation rate of the blood in the blood pump are comparatively uniform and the gradient change is comparatively small, the blood damage is effectively reduced.

Centrifuge treatment of coal tar

Energy Technology Data Exchange (ETDEWEB)

L.A. Kazak; V.Z. Kaidalov; L.F. Syrova; O.S. Miroshnichenko; A.S. Minakov [Giprokoks, the State Institute for the Design of Coke-Industry Enterprises, Kharkov (Ukraine)

2009-07-15

New technology is required for the removal of water and heavy fractions from regular coal tar. Centrifuges offer the best option. Purification of coal tar by means of centrifuges at OAO NLMK permits the production of pitch coke or electrode pitch that complies with current standards.

Centrifuges and inertial shear forces

NARCIS (Netherlands)

Loon, van J.J.W.A.; Folgering, H.T.E.; Bouten, C.V.C.; Smit, T.H.

2004-01-01

Centrifuges are often used in biological studies for 1xg control samples in space flight microgravity experiments as well as in ground based research. Using centrifugation as a tool to generate an Earth like acceleration introduces unwanted inertial shear forces to the sample. Depending on the

Seismic soil-structure interaction: Analysis and centrifuge model studies

International Nuclear Information System (INIS)

Finn, W.D.L.; Ledbetter, R.H.; Beratan, L.L.

1986-01-01

A method for nonlinear dynamic effective stress analysis applicable to soil-structure interaction problems is introduced. Full interaction including slip between structure and foundation is taken into account and the major factors that must be considered when computing dynamic soil response are included. An experimental investigation using simulated earthquake tests on centrifuged geotechnical models was conducted to obtain prototype response data of foundation soils carrying both surface and embedded structures and to validate the dynamic effective stress analysis. The centrifuge tests were conducted in the Geotechnical Centrifuge at Cambridge University, England. Horizontal and vertical accelerations were measured at various points on structures and in the sand foundation. Seismically induced pore water pressure changes were also measured at various locations in the foundation. Computer plots of the data were obtained while the centrifuge was in flight and representative samples are presented. The results clearly show the pronounced effect of increasing pore water pressures on dynamic response. It is demonstrated that a coherent picture of dynamic response of soil-structure systems is provided by dynamic effective stress nonlinear analysis. On the basis of preliminary results, it appears that the effects of pore water pressure can be predicted. (orig.)

HIRFL-SSC trim coil currents calculation by conjugate gradients method

International Nuclear Information System (INIS)

Liu, W.

2005-01-01

For accelerating different kinds of ions to various energies, the HIRFL-SSC should form the corresponding isochronous magnetic field by its main coil and trim coils. Previously, there were errors in fitting the theoretical isochronous magnetic field in the small radius region, which led to some operation difficulties for ion acceleration in the inject region. After further investigation of the restrictive condition of the maximum current limitation, the trim coil currents for fitting the theoretical isochronous magnetic field were recalculated by the conjugate gradients method. Better results were obtained in the operation of HIRFL-SSC. This article introduces the procedure to calculate the trim coil currents. The calculation method of conjugate gradients is introduced and the fitting error is analysed. (author)

Design of Structural Parameters for Centrifugal Elevator Overspeed Governors

Directory of Open Access Journals (Sweden)

Song Yunpu

2014-01-01

Full Text Available As an important part of overspeed and fail-safe protection for elevators, the centrifugal elevator overspeed governor is a device for limiting overspeed of elevator cars. This paper researches on the vibration of the centrifugal block, which plays a key role in the performance of this overspeed governor. By performing dynamics analysis on the centrifugal block, the differential equation on the vibration of the centrifugal block is established. Based on this, the paper performs simulation analysis on the influence of systematic parameters such as the speed of the overspeed governor sheave, the mass of centrifugal block, the turning radius of the centrifugal block, the position where the spring acts, and the stiffness of the centrifugal block spring, on the vibration of the centrifugal block, and finds out their specific influence relationship.

Theoretical considerations in solid bowl centrifugation

International Nuclear Information System (INIS)

Hamilton, R.T.

1979-01-01

A combination of literature survey and independent analysis determined three relationships for the prediction of the critical (or minimum recoverable) particle size in a solid bowl centrifuge. The relationships were derived based on three different theories of fluid behavior within the centrifuge; (1) laminar film flow (laminar film model), (2) plug flow (Sharples Model), and parabolic flow (modified Sharples Model). The critical particle size for the centrifuge used in Cs-PTA recovery in the CAW process predicted by the three relationships range from 0.19 to 0.34 μm (1 μm = 10 -6 m). The laminar film model gives the most conservative estimate of critical particle size (0.34 μm) and the resulting relationship is recommended for use to predict solid bowl centrifuge performance. Three correction factors are incorporated into the predictive equations to account for the effects of fluid turbulence near the centrifuge feed point, fluid lag and hindered settling. Of these factors, turbulence near the feed point (which is accounted for by using an effective centrifuge length) has the greatest impact, increasing the predicted critical particle size by 15%, while the combination of fluid lag and hindered settling factors increase the recoverable particle size by 4%. The overall effect of the correction factors is an approximately 20% decrease in centrifuge effectivity. The fraction of solids smaller than the critical size range has not been reliably determined for laboratory or plant prepared Cs-PTA. In addition, the density of Cs-PTA crystals is reported to vary from 3.2 to 12 grams per cubic centimeter

Accurate conjugate gradient methods for families of shifted systems

NARCIS (Netherlands)

Eshof, J. van den; Sleijpen, G.L.G.

We present an efficient and accurate variant of the conjugate gradient method for solving families of shifted systems. In particular we are interested in shifted systems that occur in Tikhonov regularization for inverse problems since these problems can be sensitive to roundoff errors. The

Use of a preconditioned Bi-conjugate gradient method for hybrid plasma stability analysis

International Nuclear Information System (INIS)

Mikic, Z.; Morse, E.C.

1985-01-01

The numerical stability analysis of compact toroidal plasmas using implicit time differencing requires the solution of a set of coupled, 2-dimensional, elliptic partial differential equations for the field quantities at every timestep. When the equations are spatially finite-differenced and written in matrix form, the resulting matrix is large, sparse, complex, non-Hermitian, and indefinite. The use of the preconditioned bi-conjugate gradient method for solving these equations is discussed. The effect of block-diagonal preconditioning and incomplete block-LU preconditionig on the convergence of the method is investigated. For typical matrices arising in our studies, the eigenvalue spectra of the original and preconditioned matrices are calculated as an illustration of the effectiveness of the preconditioning. We show that the preconditioned bi-conjugate gradient method coverages more rapidly than the conjugate gradient method applied to the normal equations, and that it is an effective iterative method for the class of non-Hermitian, indefinite problems of interest

Root resistance to cavitation is accurately measured using a centrifuge technique.

Science.gov (United States)

Pratt, R B; MacKinnon, E D; Venturas, M D; Crous, C J; Jacobsen, A L

2015-02-01

Plants transport water under negative pressure and this makes their xylem vulnerable to cavitation. Among plant organs, root xylem is often highly vulnerable to cavitation due to water stress. The use of centrifuge methods to study organs, such as roots, that have long vessels are hypothesized to produce erroneous estimates of cavitation resistance due to the presence of open vessels through measured samples. The assumption that roots have long vessels may be premature since data for root vessel length are sparse; moreover, recent studies have not supported the existence of a long-vessel artifact for stems when a standard centrifuge technique was used. We examined resistance to cavitation estimated using a standard centrifuge technique and compared these values with native embolism measurements for roots of seven woody species grown in a common garden. For one species we also measured vulnerability using single-vessel air injection. We found excellent agreement between root native embolism and the levels of embolism measured using a centrifuge technique, and with air-seeding estimates from single-vessel injection. Estimates of cavitation resistance measured from centrifuge curves were biologically meaningful and were correlated with field minimum water potentials, vessel diameter (VD), maximum xylem-specific conductivity (Ksmax) and vessel length. Roots did not have unusually long vessels compared with stems; moreover, root vessel length was not correlated to VD or to the vessel length of stems. These results suggest that root cavitation resistance can be accurately and efficiently measured using a standard centrifuge method and that roots are highly vulnerable to cavitation. The role of root cavitation resistance in determining drought tolerance of woody species deserves further study, particularly in the context of climate change. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

MALDI-TOF identification of Gram-negative bacteria directly from blood culture bottles containing charcoal: Sepsityper® kits versus centrifugation-filtration method.

Science.gov (United States)

Riederer, Kathleen; Cruz, Kristian; Shemes, Stephen; Szpunar, Susan; Fishbain, Joel T

2015-06-01

Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry has dramatically altered the way microbiology laboratories identify clinical isolates. Direct blood culture (BC) detection may be hampered, however, by the presence of charcoal in BC bottles currently in clinical use. This study evaluates an in-house process for extraction and MALDI-TOF identification of Gram-negative bacteria directly from BC bottles containing charcoal. Three hundred BC aliquots were extracted by a centrifugation-filtration method developed in our research laboratory with the first 96 samples processed in parallel using Sepsityper® kits. Controls were colonies from solid media with standard phenotypic and MALDI-TOF identification. The identification of Gram-negative bacteria was successful more often via the in-house method compared to Sepsityper® kits (94.7% versus 78.1%, P≤0.0001). Our in-house centrifugation-filtration method was further validated for isolation and identification of Gram-negative bacteria (95%; n=300) directly from BC bottles containing charcoal. Copyright © 2015 Elsevier Inc. All rights reserved.

Prediction and Reduction of Aerodynamic Noise of the Multiblade Centrifugal Fan

Directory of Open Access Journals (Sweden)

Shuiqing Zhou

2014-08-01

Full Text Available An aerodynamic and aeroacoustic investigation of the multiblade centrifugal fan is proposed in this paper, and a hybrid technique of combining flow field calculation and acoustic analysis is applied to solve the aeroacoustic problem of multiblade centrifugal fan. The unsteady flow field of the multiblade centrifugal fan is predicted by solving the incompressible Reynolds-averaged Navier-Stokes (RANS equations with conventional computing techniques for fluid dynamics. The principal noise source induced is extracted from the calculation of the flow field by using acoustic principles, and the modeled sources on inner and outer surfaces of the volute are calculated with multiregional boundary element method (BEM. Through qualitative analysis, the sound pressure amplitude distribution of the multiblade centrifugal fan in near field is given and the sound pressure level (SPL spectrum diagram of monitoring points in far field is obtained. Based on the analysis results, the volute tongue structure is adjusted and then a low-noise design for the centrifugal fan is proposed. The comparison of noise tests shows the noise reduction of improved fan model is more obvious, which is in good agreement with the prediction using the hybrid techniques.

Gas dynamics in strong centrifugal fields

OpenAIRE

Bogovalov, S. V.; Kislov, V. A.; Tronin, I. V.

2017-01-01

Dynamics of waves generated by scopes in gas centrifuges (GC) for isotope separation is considered. The centrifugal acceleration in the GC reaches values of the order of $10^6$g. The centrifugal and Coriolis forces modify essentially the conventional sound waves. Three families of the waves with different polarisation and dispersion exist in these conditions. Dynamics of the flow in the model GC Iguasu is investigated numerically. Comparison of the results of the numerical modelling of the wa...

Guided imagery, anxiety, heart rate, and heart rate variability during centrifuge training.

Science.gov (United States)

Jing, Xiaolu; Wu, Ping; Liu, Fang; Wu, Bin; Miao, Danmin

2011-02-01

Centrifuge training is an important method of improving the hypergravity tolerance of pilots, cosmonauts, and Chinese astronauts. However, the concomitants of tension or anxiety often impede training. Guided imagery (GI), a mind-body relaxation technique, provides a behavioral and cognitive means whereby individuals are able to exert control over the focus of attention. This study aims to investigate the immediate effects of GI for reducing stress in centrifuge training. There were 12 healthy young men who were randomly assigned to a GI group or music group. We measured changes in heart rate during centrifuge training, in heart rate variability before and after centrifuge training, and also evaluated relaxation and anxiety in three phases: before intervention, after intervention, and following centrifuge training. The change in the pattern of anxiety was different in the two groups over the three phases. Anxiety (measured by State Anxiety Inventory) in the GI group changed from 31.7 +/- 5.9 to 26.8 +/- 2.6 and 27.8 +/- 4.1, whereas for the music group this changed from 32.2 +/- 7.6 to 31.2 +/- 8.3 and 26.8 +/- 6.8. During centrifuge training, the maximal HR for the GI group (101.2 +/- 8.8) was lower than that of the music group (123.0 +/- 19.1). In addition GI showed a decrease in low frequency (LF, 0.04-0.15 Hz) components and an increase in high frequency (HF, 0.15-0.4 Hz) components before and after centrifuge training. GI was capable of decreasing tension, anxiety, and sympathetic nervous system activity pre- or post-centrifugation.

Return to Flying Duties Following Centrifuge or Vibration Exposures

Science.gov (United States)

Scheuring, Richard A.; Clarke, Jonathan; Jones, Jeffrey A.

2009-01-01

Introduction: In an effort to determine the human performance limits for vibration in spacecraft being developed by NASA, astronauts were evaluated during a simulated launch profile in a centrifuge/vibration environment and separate vibration-only simulation. Current USAF and Army standards for return to flight following centrifuge exposures require 12-24 hours to pass before a crewmember may return to flying duties. There are no standards on vibration exposures and return to flying duties. Based on direct observation and provocative neurological testing of the astronauts, a new standard for return to flying duties following centrifuge and/or vibration exposures was established. Methods: 13 astronaut participants were exposed to simulated launch profiles in a + 3.5 Gx bias centrifuge/vibration environment and separately on a vibration table at the NASA-Ames Research Center. Each subject had complete neurological evaluations pre- and post-exposure for the centrifuge/vibration runs with the NASA neurological function rating scale (NFRS). Subjects who participated in the vibration-only exposures had video oculography performed with provocative maneuvers in addition to the NFRS. NFRS evaluations occurred immediately following each exposure and at 1 hour post-run. Astronauts who remained symptomatic at 1 hour had repeat NFRS performed at 1 hour intervals until the crewmember was asymptomatic. Results: Astronauts in the centrifuge/vibration study averaged a 3-5 point increase in NFRS scores immediately following exposure but returned to baseline 3 hours post-run. Subjects exposed to the vibration-only simulation had a 1-3 point increase following exposure and returned to baseline within 1-2 hours. Pre- and post- vibration exposure video oculography did not reveal any persistent ocular findings with provocative testing 1 hour post-exposure. Discussion: Based on direct observations and objective measurement of neurological function in astronauts following simulated launch

An Application of the Acoustic Similarity Law to the Numerical Analysis of Centrifugal Fan Noise

Science.gov (United States)

Jeon, Wan-Ho; Lee, Duck-Joo; Rhee, Huinam

Centrifugal fans, which are frequently used in our daily lives and various industries, usually make severe noise problems. Generally, the centrifugal fan noise consists of tones at the blade passing frequency and its higher harmonics. These tonal sounds come from the interaction between the flow discharged from the impeller and the cutoff in the casing. Prediction of the noise from a centrifugal fan becomes more necessary to optimize the design to meet both the performance and noise criteria. However, only some limited studies on noise prediction method exist because there are difficulties in obtaining detailed information about the flow field and casing effect on noise radiation. This paper aims to investigate the noise generation mechanism of a centrifugal fan and to develop a prediction method for the unsteady flow and acoustic pressure fields. In order to do this, a numerical analysis method using acoustic similarity law is proposed, and it is verified that the method can predict the noise generation mechanism very well by comparing the predicted results with available experimental results.

Synchronization of Budding Yeast by Centrifugal Elutriation.

Science.gov (United States)

Rosebrock, Adam P

2017-01-03

In yeast, cell size is normally tightly linked to cell cycle progression. Centrifugal elutriation is a method that fractionates cells based on the physical properties of cell size-fluid drag and buoyant density. Using a specially modified centrifuge and rotor system, cells can be physically separated into one or more cohorts of similar size and therefore cell cycle position. Small G 1 daughters are collected first, followed by successively larger cells. Elutriated populations can be analyzed immediately or can be returned to medium and permitted to synchronously progress through the cell cycle. This protocol describes two different elutriation methods. In the first, one or more fractions of synchronized cells are obtained from an asynchronous starting population, reincubated, and followed prospectively across a time series. In the second, an asynchronous starting population is separated into multiple fractions of similarly sized cells, and each cohort of similarly sized cells can be analyzed separately without further growth. © 2017 Cold Spring Harbor Laboratory Press.

Liquid centrifugation for nuclear waste partitioning

International Nuclear Information System (INIS)

Bowman, C.D.

1992-01-01

The performance of liquid centrifugation for nuclear waste partitioning is examined for the Accelerator Transmutation of Waste Program currently under study at the Los Alamos National Laboratory. Centrifugation might have application for the separation of the LiF-BeF 2 salt from heavier radioactive materials fission product and actinides in the separation of fission product from actinides, in the isotope separation of fission-product cesium before transmutation of the 137 Cs and 135 Cs, and in the removal of spallation product from the liquid lead target. It is found that useful chemical separations should be possible using existing materials for the centrifuge construction for all four cases with the actinide fraction in fission product perhaps as low as 1 part in 10 7 and the fraction of 137 CS in 133 Cs being as low as a few parts in 10 5 . A centrifuge cascade has the advantage that it can be assembled and operated as a completely closed system without a waste stream except that associated with maintenance or replacement of centrifuge components

Radial loads and axial thrusts on centrifugal pumps

International Nuclear Information System (INIS)

Anon.

1986-01-01

The proceedings of a seminar organised by the Power Industries Division of the IMechE are presented in this text. Complete contents: Review of parameters influencing hydraulic forces on centrifugal impellers; The effect of fluid forces at various operation conditions on the vibrations of vertical turbine pumps; A review of the pump rotor axial equilibrium problem - some case studies; Dynamic hydraulic loading on a centrifugal pump impeller; Experimental research on axial thrust loads of double suction centrifugal pumps; A comparison of pressure distribution and radial loads on centrifugal pumps; A theoretical and experimental investigation of axial thrusts within a multi-stage centrifugal pump

Analysis of static characteristic roots and propagation of disturbance of adjustable centrifuge cascade

International Nuclear Information System (INIS)

Li Weijie; Wu Zhongdi; Nong Guowei; Zeng Shi

2014-01-01

The hydraulic characteristic roots of a centrifuge cascade represent an important property of the cascade performance. Regulators and centrifuges are the key components that have a significant influence on the cascade hydraulic performance. The method used in diffusion cascades was adopted to obtain the static characteristic roots by solving the small disturbance equation for an adjustable centrifuge cascade in which all stages have the same fluid parameters. As the light stream flowrate of a centrifuge is irrelevant to the pressure at the outlet of the light flow, and the heavy stream flows at the speed of sound, there are only 2 static characteristic roots in the centrifuge cascade: the first root Z_1 is the main characteristic root and the second one Z_2 comes into play only when there exists a feed. The value of the main characteristic root is influenced by the amplification factor of the regulators, the fluid resistance in the main feed pipe and other factors. When Z_1 is smaller than 1, it increases with the fluid resistance. A large enough amplification factor has little impact on Z_1. The same distribution of the relative changes of the light fraction along the cascade is obtained by an analytical and a numerical method. (authors)

Centrifuge modeling of one-step outflow tests for unsaturated parameter estimations

Directory of Open Access Journals (Sweden)

H. Nakajima

2006-01-01

Full Text Available Centrifuge modeling of one-step outflow tests were carried out using a 2-m radius geotechnical centrifuge, and the cumulative outflow and transient pore water pressure were measured during the tests at multiple gravity levels. Based on the scaling laws of centrifuge modeling, the measurements generally showed reasonable agreement with prototype data calculated from forward simulations with input parameters determined from standard laboratory tests. The parameter optimizations were examined for three different combinations of input data sets using the test measurements. Within the gravity level examined in this study up to 40g, the optimized unsaturated parameters compared well when accurate pore water pressure measurements were included along with cumulative outflow as input data. With its capability to implement variety of instrumentations under well controlled initial and boundary conditions and to shorten testing time, the centrifuge modeling technique is attractive as an alternative experimental method that provides more freedom to set inverse problem conditions for the parameter estimation.

Centrifuge modeling of one-step outflow tests for unsaturated parameter estimations

Science.gov (United States)

Nakajima, H.; Stadler, A. T.

2006-10-01

Centrifuge modeling of one-step outflow tests were carried out using a 2-m radius geotechnical centrifuge, and the cumulative outflow and transient pore water pressure were measured during the tests at multiple gravity levels. Based on the scaling laws of centrifuge modeling, the measurements generally showed reasonable agreement with prototype data calculated from forward simulations with input parameters determined from standard laboratory tests. The parameter optimizations were examined for three different combinations of input data sets using the test measurements. Within the gravity level examined in this study up to 40g, the optimized unsaturated parameters compared well when accurate pore water pressure measurements were included along with cumulative outflow as input data. With its capability to implement variety of instrumentations under well controlled initial and boundary conditions and to shorten testing time, the centrifuge modeling technique is attractive as an alternative experimental method that provides more freedom to set inverse problem conditions for the parameter estimation.

Centrifugal separator cascade connected in zigzag manner

International Nuclear Information System (INIS)

Kai, Tsunetoshi; Inoue, Yoshiya; Oya, Akio; Nagakura, Masaaki.

1974-01-01

Object: To effectively accommodate centrifugal separators of the entire cascade within the available space in a plant by freely selecting perpendicular direction of connection of the centrifugal separator. Structure: Centrifugal separators are connected in zigzag fashion by using a single header for each stage so that in a rectangular shape the entire cascade is arranged. (Kamimura, M.)

Mathematical and physical modeling of rainfall in centrifuge

OpenAIRE

CAICEDO, Bernardo; THOREL, Luc; TRISTANCHO, Julian

2015-01-01

Rainfall simulation in centrifuge models is important for modelling soil-atmosphere interactions. However, the presence of Coriolis force, drag forces, evaporation and wind within the centrifuge may affect the distribution of rainfall over the model. As a result, development of appropriate centrifuge rain simulators requires a demanding process of experimental trial and error. This paper highlights the key factors involved in controlling rainfall in centrifuge simulations, develops a mathemat...

Centrifuge advances using HTS magnetic bearings

Science.gov (United States)

Werfel, F. N.; Flögel-Delor, U.; Rothfeld, R.; Wippich, D.; Riedel, T.

2001-05-01

Passive magnetic bearings are of increasing technical interest. We performed experiments with centrifugal rotors to analyze gyroscopic forces in terms imbalance, rotor elasticity and damping. Centrifuge rotors need to be operated soft and stable without whirling the sediments. In order to evaluate optimal parameters critical and resonance behaviors are investigated. Eccentricities up 2 mm are safely passed by accelerating test wheels. In a simple model we describe the effect of passing critical rotational speeds. Measurements of bearing properties and wheel performance are presented. We have constructed a first prototype centrifuge designed with a HTS double bearing which operates a titanium rotor safely up to 30 000 rpm. A 15 W Stirling cooler serves cryogenics of the YBCO stators. From the experiments design guidelines for centrifugal applications with HTS bearings are given.

THE EFFICIENCY OF YARN WOUND FILTERS IN GIARDIA CYSTS REMOVAL USING THE IMPROVED SUCROSE GRADIENT

Directory of Open Access Journals (Sweden)

M.R SHAH MANSOURI

2001-09-01

89.95±4.25 . Discussion. The comparison of the efficiency obtained with those obtained across the world from the application of other techniques associated with this method indicated that this filter was advantages to similar filters, keeping in mind also that this filter can be used in field situations. It is essential to modify in each region the method of cyst removal from sediments using Sucrose gradient according to the particular morphological characteristics and density of cystes. The optimization of Sucrose gradient and the centrifugation duration and speed seems equally important.

Centrifuge workers study. Phase II, completion report

International Nuclear Information System (INIS)

Wooten, H.D.

1994-09-01

Phase II of the Centrifuge Workers Study was a follow-up to the Phase I efforts. The Phase I results had indicated a higher risk than expected among centrifuge workers for developing bladder cancer when compared with the risk in the general population for developing this same type of cancer. However, no specific agent could be identified as the causative agent for these bladder cancers. As the Phase II Report states, Phase I had been limited to workers who had the greatest potential for exposure to substances used in the centrifuge process. Phase II was designed to expand the survey to evaluate the health of all employees who had ever worked in Centrifuge Program Departments 1330-1339 but who had not been interviewed in Phase I. Employees in analytical laboratories and maintenance departments who provided support services for the Centrifuge Program were also included in Phase II. In December 1989, the Oak Ridge Associated Universities (ORAU), now known as Oak Ridge Institute for Science and Education (ORISE), was contracted to conduct a follow-up study (Phase II). Phase H of the Centrifuge Workers Study expanded the survey to include all former centrifuge workers who were not included in Phase I. ORISE was chosen because they had performed the Phase I tasks and summarized the corresponding survey data therefrom

Centrifuge workers study. Phase II, completion report

Energy Technology Data Exchange (ETDEWEB)

Wooten, H.D.

1994-09-01

Phase II of the Centrifuge Workers Study was a follow-up to the Phase I efforts. The Phase I results had indicated a higher risk than expected among centrifuge workers for developing bladder cancer when compared with the risk in the general population for developing this same type of cancer. However, no specific agent could be identified as the causative agent for these bladder cancers. As the Phase II Report states, Phase I had been limited to workers who had the greatest potential for exposure to substances used in the centrifuge process. Phase II was designed to expand the survey to evaluate the health of all employees who had ever worked in Centrifuge Program Departments 1330-1339 but who had not been interviewed in Phase I. Employees in analytical laboratories and maintenance departments who provided support services for the Centrifuge Program were also included in Phase II. In December 1989, the Oak Ridge Associated Universities (ORAU), now known as Oak Ridge Institute for Science and Education (ORISE), was contracted to conduct a follow-up study (Phase II). Phase H of the Centrifuge Workers Study expanded the survey to include all former centrifuge workers who were not included in Phase I. ORISE was chosen because they had performed the Phase I tasks and summarized the corresponding survey data therefrom.

Gradient augmented level set method for phase change simulations

Science.gov (United States)

Anumolu, Lakshman; Trujillo, Mario F.

2018-01-01

A numerical method for the simulation of two-phase flow with phase change based on the Gradient-Augmented-Level-set (GALS) strategy is presented. Sharp capturing of the vaporization process is enabled by: i) identification of the vapor-liquid interface, Γ (t), at the subgrid level, ii) discontinuous treatment of thermal physical properties (except for μ), and iii) enforcement of mass, momentum, and energy jump conditions, where the gradients of the dependent variables are obtained at Γ (t) and are consistent with their analytical expression, i.e. no local averaging is applied. Treatment of the jump in velocity and pressure at Γ (t) is achieved using the Ghost Fluid Method. The solution of the energy equation employs the sub-grid knowledge of Γ (t) to discretize the temperature Laplacian using second-order one-sided differences, i.e. the numerical stencil completely resides within each respective phase. To carefully evaluate the benefits or disadvantages of the GALS approach, the standard level set method is implemented and compared against the GALS predictions. The results show the expected trend that interface identification and transport are predicted noticeably better with GALS over the standard level set. This benefit carries over to the prediction of the Laplacian and temperature gradients in the neighborhood of the interface, which are directly linked to the calculation of the vaporization rate. However, when combining the calculation of interface transport and reinitialization with two-phase momentum and energy, the benefits of GALS are to some extent neutralized, and the causes for this behavior are identified and analyzed. Overall the additional computational costs associated with GALS are almost the same as those using the standard level set technique.

Boundary layers affected by different pressure gradients investigated computationally by a zonal RANS-LES method

International Nuclear Information System (INIS)

Roidl, B.; Meinke, M.; Schröder, W.

2014-01-01

Highlights: • Reformulated synthetic turbulence generation method (RSTGM) is applied. • Zonal RANS-LES method is applied to boundary layers at pressure gradients. • Good agreement with the pure LES and other reference data is obtained. • The RSTGM is applicable to pressure gradient flows without modification. • RANS-to-LES boundary should be located where -1·10 6 6 is satisfied. -- Abstract: The reformulated synthetic turbulence generation (RSTG) method is used to compute by a fully coupled zonal RANS-LES approach turbulent non-zero-pressure gradient boundary layers. The quality of the RSTG method, which is based on the same shape functions and length scale distributions as in zero-pressure gradient flow, is discussed by comparing the zonal RANS-LES findings with pure LES, pure RANS, direct numerical simulation (DNS), and experimental data. For the favorable pressure gradient (FPG) simulation the RANS-to-LES transition occurs in the accelerated flow region and for the adverse pressure gradient (APG) case it is located in the decelerated flow region. The results of the time and spanwise averaged skin-friction distributions, velocity profiles, and Reynolds stress distributions of the zonal RANS-LES simulation show a satisfactory to good agreement with the pure LES, reference DNS, and experimental data. The quality of the findings shows that the rigorous formulation of the synthetic turbulence generation makes the RSTG method applicable without a priori knowledge of the flow properties but those determined by the RANS solution and without using additional control planes to regulate the shear stress budget to a wide range of Reynolds numbers and pressure gradients. The method is a promising approach to formulate embedded RANS-to-LES boundaries in flow regions where the Pohlhausen or acceleration parameter satisfies -1·10 -6 ⩽K⩽2·10 -6

Development of a 3D circular microfluidic centrifuge for the separation of mixed particles by using their different centrifuge times

International Nuclear Information System (INIS)

Jeon, H J; Kim, D I; Kim, M J; Nguyen, X D; Park, D H; Go, J S

2015-01-01

This paper presents a circular microfluidic centrifuge with two inlets and two outlets to separate mixed microparticles with a specially designed sample injection hole. To separate the mixed particles, it uses a rotational flow, generated in a chamber by counter primary flows in the microchannels. The shape and sizes of the circular microfluidic centrifuge have been designed through numerical evaluation to have a large relative centrifugal force. The difference of centrifuge times of the mixed particles of 1 μm and 6 μm was determined to be 8.2 s at an inlet Reynolds number of 500 and a sample Reynolds number of 20. In the experiment, this was measured to be about 10 s. From the separation of the two polymer particles analogous to the representative sizes of platelets and red blood cells, the circular microfluidic centrifuge shows a potential to separate human blood cells size-selectively by using the difference of centrifuge times. (paper)

Waves in Strong Centrifugal Field

Science.gov (United States)

Bogovalov, S. V.; Kislov, V. A.; Tronin, I. V.

Dynamics of waves generated by scopes in gas centrifuges (GC) for isotope separation is considered. The centrifugal acceleration in the GC reaches values of the order of 106g. The centrifugal and Coriolis forces modify essentially the conventional sound waves. Three families of the waves with different polarization and dispersion exist in these conditions. Dynamics of the flow in the model GC Iguasu is investigated numerically. Comparison of the results of the numerical modeling of the wave dynamics with the analytical predictions is performed. New phenomena of the resonances in the GC is found. The resonances occur for the waves polarized along the rotational axis having the smallest dumping due to the viscosity.

Simplified simulation of multicomponent isotope separation by gas centrifuge

International Nuclear Information System (INIS)

Guo Zhixiong; Ying Chuntong

1995-01-01

The expressions of diffusion equation for multicomponent isotope separation by gas centrifuge are derived by utilizing the simplified diffusion transport vector. A method of radial averaging which was restricted to a binary mixture is extended to multicomponent isotope mixtures by using an iterative scheme. A numerical analysis of tetradic UF 6 or SF 6 gas isotope separation by centrifuge is discussed when a special model of velocity distribution is given. The dependence of mutual separation factor for the components on their molecular weights' difference is obtained. Some aspects of the given model of gas fluid are also discussed

Review of the gas centrifuge until 1962. Part I: Principles of separation physics

International Nuclear Information System (INIS)

Whitley, S.

1984-01-01

There are two sets of principles involved in the development of the gas centrifuge, the internal separation physics and the external means of spinning a rotor at very high speeds. Only the first aspect is discussed in this part of the review. First, the industrial requirement for the separation of the uranium isotopes is defined so that the separation history can be put in a modern perspective. The history of separation physics itself is then traced back to the theory of centrifugal force by Huygens and the equivalence of this force to that of gravity. The barometric equation giving the variation of atmospheric pressure with height and the law of partial pressures can then be adapted to the centrifuge to give the steady-state theory of separation. This work was completed in the last century but was not confirmed in its application to isotope separation until 1936. The detailed separation physics for non-steady-state conditions required for a production centrifuge was developed during the American wartime Manhattan Project. During this work the theory giving the maximum output of a centrifuge was developed by Dirac, and soon afterwards Cohen and Kaplan showed that the best method of operation for a production centrifuge is in a countercurrent mode of operation. This method gives a large separation factor at relatively small flow rates through the rotor. The theory of how to set up an internal countercurrent was given by Martin during an equivalent wartime German project, and refinements to the theory, showing how the countercurrent persists along a centrifuge rotor, was given by Dirac and Steenbeck, the latter during a postwar Russian project. This theory was extended by Parker, Ging, and Mayo of the University of Virginia, whose work was completed by 1962, the limit of this review

Boundedness and convergence of online gradient method with penalty for feedforward neural networks.

Science.gov (United States)

Zhang, Huisheng; Wu, Wei; Liu, Fei; Yao, Mingchen

2009-06-01

In this brief, we consider an online gradient method with penalty for training feedforward neural networks. Specifically, the penalty is a term proportional to the norm of the weights. Its roles in the method are to control the magnitude of the weights and to improve the generalization performance of the network. By proving that the weights are automatically bounded in the network training with penalty, we simplify the conditions that are required for convergence of online gradient method in literature. A numerical example is given to support the theoretical analysis.

NASA low speed centrifugal compressor

Science.gov (United States)

Hathaway, Michael D.

1990-01-01

The flow characteristics of a low speed centrifugal compressor were examined at NASA Lewis Research Center to improve understanding of the flow in centrifugal compressors, to provide models of various flow phenomena, and to acquire benchmark data for three dimensional viscous flow code validation. The paper describes the objectives, test facilities' instrumentation, and experiment preliminary comparisons.

An M-step preconditioned conjugate gradient method for parallel computation

Science.gov (United States)

Adams, L.

1983-01-01

This paper describes a preconditioned conjugate gradient method that can be effectively implemented on both vector machines and parallel arrays to solve sparse symmetric and positive definite systems of linear equations. The implementation on the CYBER 203/205 and on the Finite Element Machine is discussed and results obtained using the method on these machines are given.

Separation of uranium isotopes by gas centrifugation

International Nuclear Information System (INIS)

Jordan, I.

1980-05-01

The uranium isotope enrichment is studied by means of the countercurrent gas centrifuge driven by thermal convection. A description is given of (a) the transfer and purification of the uranium hexafluoride used as process gas in the present investigation; (b) the countercurrent centrifuge ZG3; (c) the system designed for the introduction and extraction of the process gas from the centrifuge; (d) the measurement of the process gas flow rate through the centrifuge; (e) the determination of the uranium isotopic abundance by mass spectrometry; (f) the operation and mechanical behavior of the centrifuge and (g) the isotope separation experiments, performed, respectively, at total reflux and with production of enriched material. The results from the separation experiments at total reflux are discussed in terms of the enrichment factor variation with the magnitude and flow profile of the countercurrent given by the temperature difference between the rotor covers. As far as the separation experiments with production are concerned, the discussion of their results is presented through the variation of the enrichment factor as a function of the flow rate, the observed asymmetry of the process and the calculated separative power of the centrifuge. (Author) [pt

Unexpected properties of the centrifugal force

International Nuclear Information System (INIS)

Abramowicz, M.A.

1990-01-01

Contrary to what is stated in the Newtonian dynamics, rotation of a reference frame is not sufficient for the occurrence of the centrifugal force. Instead, the necessary and sufficient condition is a motion along a path different from that of a photon trajectory in space. This calls for a rather fundamental change in understanding of the very nature of the centrifugal force. It also has important practical physical consequences: in a strong gravitational field, where light trajectories are substantially curved, centrifugal force is much weaker than the Newtonian theory predicts. In addition, when there are closed (circular) photon trajectories in space, the centrifugal force may reverse its direction - it attracts towards the rotation axis!. The weakening of the centrifugal force in strong gravitational fields and the reversal of its direction in the neighbourhood of close photon trajectories in space fully and clearly explain puzzling examples of counter intuitive behaviour of dynamical effects of rotation found previously by several authors: e.g. reversal of the ellipticity behaviour of the relativistic Maclaurin spheroids (Chandrasekhar and Miller, 1974), reversal of the viscous torque action (Anderson and Lemos, 1988), or the fact that rotation increases internal pressure of a sufficiently compact star (Abramowicz and Wagoner, 1974). Weakening of the centrifugal force implies that rotating neutron stars are less oblate (and probably more stable) than the Newtonian theory predicts. This is important for the recently discussed question of how fast can pulsars spin. (author). 23 refs, 3 figs

Unexpected properties of the centrifugal force

International Nuclear Information System (INIS)

Abramowicz, M.A.

1990-01-01

Contrary to what is stated in the Newtonian dynamics, rotation of a reference frame is not sufficient for the occurrence of the centrifugal force. Instead, the necessary and sufficient condition is a motion along a path different from that of a photon trajectory in space. This calls for a rather fundamental change in understanding of the very nature of the centrifugal force. It also has important practical physical consequences: in a strong gravitational field, where light trajectories are substantially curved, centrifugal force is much weaker than the Newtonian theory predicts. In addition, when there are closed (circular) photon trajectories in space, the centrifugal force may reverse its direction - it attracts towards the rotation axis. The weakening of the centrifugal force in strong gravitational fields and the reversal of its direction in the neighborhood of close photon trajectories in space fully and clearly explain puzzling examples of counter intuitive behaviour of dynamical effects of rotation found previously by several authors: e.g. reversal of the ellipticity behaviour of the relativistic Maclaurin spheroids (Chandrasekhar and Miller, 1974), reversal of the viscous torque action (Anderson and Lemos, 1988) or the fact that rotation increases internal pressure of a sufficiently compact star (Abramowicz and Wagoner, 1974). Weakening of the centrifugal force implies that rotating neutron stars are less oblate (and probably more stable) than the Newtonian theory predicts. This is important for the recently discussed question of how fast can pulsars spins. (author). 31 refs, 3 figs

Blood culture bottles are superior to lysis-centrifugation tubes for bacteriological diagnosis of spontaneous bacterial peritonitis.

OpenAIRE

Siersema, P D; de Marie, S; van Zeijl, J H; Bac, D J; Wilson, J H

1992-01-01

The conventional method of ascitic fluid culturing was compared with the bedside inoculation of ascites into blood culture bottles and into lysis-centrifugation tubes. The conventional culture method was compared with the blood culture bottle method in 31 episodes of spontaneous bacterial peritonitis (SBP). Cultures were positive with the conventional culture method in 11 (35%) episodes and with the blood culture bottle method in 26 (84%) episodes (P less than 0.001). The lysis-centrifugation...

Bacterial Cell Surface Damage Due to Centrifugal Compaction

NARCIS (Netherlands)

Peterson, Brandon W.; Sharma, Prashant K.; van der Mei, Henny C.; Busscher, Henk J.

Centrifugal damage has been known to alter bacterial cell surface properties and interior structures, including DNA. Very few studies exist on bacterial damage caused by centrifugation because of the difficulty in relating centrifugation speed and container geometry to the damage caused. Here, we

Centrifuge Facility for the International Space Station Alpha

Science.gov (United States)

Johnson, Catherine C.; Hargens, Alan R.

1994-01-01

The Centrifuge Facility planned for the International Space Station Alpha has under-one considerable redesign over the past year, primarily because the Station is now viewed as a 10 year mission rather than a 30 year mission and because of the need to simply the design to meet budget constraints and a 2000 launch date. The basic elements of the Centrifuge Facility remain the same, i.e., a 2.5 m diameter centrifuge, a micro-g holding unit, plant and animal habitats, a glovebox and a service unit. The centrifuge will still provide the full range of artificial gravity from 0.01 a to 2 - as originally planned; however, the extractor to permit withdrawal of habitats from the centrifuge without stopping the centrifuge has been eliminated. The specimen habitats have also been simplified and are derived from other NASA programs. The Plant Research Unit being developed by the Gravitational Biology Facility will be used to house plants in the Centrifuge Facility. Although not as ambitious as the Centrifuge Facility plant habitat, it will provide much better environmental control and lighting than the current Shuttle based Plant Growth Facility. Similarly, rodents will be housed in the Advanced Animal Habitat being developed for the Shuttle program. The Centrifuge Facility and ISSA will provide the opportunity to perform repeatable, high quality science. The long duration increments available on the Station will permit multigeneration studies on both plants and animals which have not previously been possible. The Centrifuge Facility will accommodate sufficient number of specimens to permit statistically significant sampling of specimens to investigate the time course of adaptation to altered gravity environments. The centrifuge will for the first time permit investigators to use gravity itself as a tool to investigate fundamental processes, to investigate the intensity and duration of gravity to maintain normal structure and function, to separate the effects of micro-g from

Conjugate-gradient preconditioning methods for shift-variant PET image reconstruction.

Science.gov (United States)

Fessler, J A; Booth, S D

1999-01-01

Gradient-based iterative methods often converge slowly for tomographic image reconstruction and image restoration problems, but can be accelerated by suitable preconditioners. Diagonal preconditioners offer some improvement in convergence rate, but do not incorporate the structure of the Hessian matrices in imaging problems. Circulant preconditioners can provide remarkable acceleration for inverse problems that are approximately shift-invariant, i.e., for those with approximately block-Toeplitz or block-circulant Hessians. However, in applications with nonuniform noise variance, such as arises from Poisson statistics in emission tomography and in quantum-limited optical imaging, the Hessian of the weighted least-squares objective function is quite shift-variant, and circulant preconditioners perform poorly. Additional shift-variance is caused by edge-preserving regularization methods based on nonquadratic penalty functions. This paper describes new preconditioners that approximate more accurately the Hessian matrices of shift-variant imaging problems. Compared to diagonal or circulant preconditioning, the new preconditioners lead to significantly faster convergence rates for the unconstrained conjugate-gradient (CG) iteration. We also propose a new efficient method for the line-search step required by CG methods. Applications to positron emission tomography (PET) illustrate the method.

Enhanced infectivity of bluetongue virus in cell culture by centrifugation.

OpenAIRE

Sundin, D R; Mecham, J O

1989-01-01

The effects of centrifugation of the infection of cell culture with bluetongue virus (BTV) were investigated. Baby hamster kidney cells were infected with BTV with or without centrifugation. Viral antigen was detected by immunofluorescence at 24 h in both centrifuged and noncentrifuged cultures. However, after 24 h of infection, the production of PFU in centrifuged cell cultures was 10- to 20-fold greater than that seen in cultures not centrifuged. In addition, centrifugation enhanced the dir...

An Improved Local Gradient Method for Sea Surface Wind Direction Retrieval from SAR Imagery

Directory of Open Access Journals (Sweden)

Lizhang Zhou

2017-06-01

Full Text Available Sea surface wind affects the fluxes of energy, mass and momentum between the atmosphere and ocean, and therefore regional and global weather and climate. With various satellite microwave sensors, sea surface wind can be measured with large spatial coverage in almost all-weather conditions, day or night. Like any other remote sensing measurements, sea surface wind measurement is also indirect. Therefore, it is important to develop appropriate wind speed and direction retrieval models for different types of microwave instruments. In this paper, a new sea surface wind direction retrieval method from synthetic aperture radar (SAR imagery is developed. In the method, local gradients are computed in frequency domain by combining the operation of smoothing and computing local gradients in one step to simplify the process and avoid the difference approximation. This improved local gradients (ILG method is compared with the traditional two-dimensional fast Fourier transform (2D FFT method and local gradients (LG method, using interpolating wind directions from the European Centre for Medium-Range Weather Forecast (ECMWF reanalysis data and the Cross-Calibrated Multi-Platform (CCMP wind vector product. The sensitivities to the salt-and-pepper noise, the additive noise and the multiplicative noise are analyzed. The ILG method shows a better performance of retrieval wind directions than the other two methods.

Assessment of parameters of gas centrifuge and separation cascade basing on integral characteristics of separation plant

Energy Technology Data Exchange (ETDEWEB)

Borisevich, Valentin, E-mail: VDBorisevich@mephi.ru [National Research Nuclear University MEPhI, Kashirskoe Shosse 31, Moscow 115409 (Russian Federation); National Research Center “Kurchatov Institute”, Kurchatov Square 1, Moscow 123182 (Russian Federation); Borshchevskiy, Michael, E-mail: Michael_mephi@mail.ru [National Research Nuclear University MEPhI, Kashirskoe Shosse 31, Moscow 115409 (Russian Federation); Andronov, Igor, E-mail: andronov@imp.kiae.ru [National Research Center “Kurchatov Institute”, Kurchatov Square 1, Moscow 123182 (Russian Federation); Senchenkov, Sergey, E-mail: senchenkov@imp.kiae.ru [National Research Center “Kurchatov Institute”, Kurchatov Square 1, Moscow 123182 (Russian Federation)

2013-12-15

Highlights: • We developed the calculation method to assess a feed flow rate into a gas centrifuge. • It is based on the knowledge of the integral characteristics of a separation plant. • Our method is verified by comparison with the results of the independent one. • The method also allows to specify other features of the separation cascade work. - Abstract: A calculation technique to assess a feed flow rate into a single GC, a total number of centrifuges in a separation cascade and to determine its likely configurations basing on the known integral characteristics of a centrifugal plant is developed. Evaluation of characteristics of the industrial gas centrifuge TC-12 and separation cascades of the NEF plant performed by two independent calculation techniques demonstrates their satisfactory agreement. This methodology would help to some extent the nuclear inspectors in evaluating and assessing the capability of an enrichment facility, and discovering any use for undeclared purposes.

Method of operating a centrifugal plasma arc furnace

International Nuclear Information System (INIS)

Kujawa, S.T.; Battleson, D.M.; Rademacher, E.L. Jr.; Cashell, P.V.; Filius, K.D.; Flannery, P.A.; Whitworth, C.G.

1998-01-01

A centrifugal plasma arc furnace is used to vitrify contaminated soils and other waste materials. An assessment of the characteristics of the waste is performed prior to introducing the waste into the furnace. Based on the assessment, a predetermined amount of iron is added to each batch of waste. The waste is melted in an oxidizing atmosphere into a slag. The added iron is oxidized into Fe 3 O 4 . Time of exposure to oxygen is controlled so that the iron does not oxidize into Fe 2 O 3 . Slag in the furnace remains relatively non-viscous and consequently it pours out of the furnace readily. Cooled and solidified slag produced by the furnace is very resistant to groundwater leaching. The slag can be safely buried in the earth without fear of contaminating groundwater. 3 figs

Conjugate gradient type methods for linear systems with complex symmetric coefficient matrices

Science.gov (United States)

Freund, Roland

1989-01-01

We consider conjugate gradient type methods for the solution of large sparse linear system Ax equals b with complex symmetric coefficient matrices A equals A(T). Such linear systems arise in important applications, such as the numerical solution of the complex Helmholtz equation. Furthermore, most complex non-Hermitian linear systems which occur in practice are actually complex symmetric. We investigate conjugate gradient type iterations which are based on a variant of the nonsymmetric Lanczos algorithm for complex symmetric matrices. We propose a new approach with iterates defined by a quasi-minimal residual property. The resulting algorithm presents several advantages over the standard biconjugate gradient method. We also include some remarks on the obvious approach to general complex linear systems by solving equivalent real linear systems for the real and imaginary parts of x. Finally, numerical experiments for linear systems arising from the complex Helmholtz equation are reported.

Performance prediction of industrial centrifuges using scale-down models.

Science.gov (United States)

Boychyn, M; Yim, S S S; Bulmer, M; More, J; Bracewell, D G; Hoare, M

2004-12-01

Computational fluid dynamics was used to model the high flow forces found in the feed zone of a multichamber-bowl centrifuge and reproduce these in a small, high-speed rotating disc device. Linking the device to scale-down centrifugation, permitted good estimation of the performance of various continuous-flow centrifuges (disc stack, multichamber bowl, CARR Powerfuge) for shear-sensitive protein precipitates. Critically, the ultra scale-down centrifugation process proved to be a much more accurate predictor of production multichamber-bowl performance than was the pilot centrifuge.

Cardiovascular Responses of Snakes to Gravitational Gradients

Science.gov (United States)

Hsieh, Shi-Tong T.; Lillywhite, H. B.; Ballard, R. E.; Hargens, A. R.; Holton, Emily M. (Technical Monitor)

1998-01-01

Snakes are useful vertebrates for studies of gravitational adaptation, owing to their elongate body and behavioral diversification. Scansorial species have evolved specializations for regulating hemodynamics during exposure to gravitational stress, whereas, such adaptations are less well developed in aquatic and non-climbing species. We examined responses of the amphibious snake,\\italicize (Nerodia rhombifera), to increments of Gz (head-to-tail) acceleration force on both a short- and long-arm centrifuge (1.5 vs. 3.7 m radius, from the hub to tail end of snake). We recorded heart rate, dorsal aortic pressure, and carotid arterial blood flow during stepwise 0.25 G increments of Gz force (referenced at the tail) in conscious animals. The Benz tolerance of a snake was determined as the Gz level at which carotid blood flow ceased and was found to be significantly greater at the short- than long-arm centrifuge radius (1.57 Gz vs. 2.0 Gz, respectively; P=0.016). A similar pattern of response was demonstrated in semi-arboreal rat snakes,\\italicize{Elaphe obsoleta}, which are generally more tolerant of Gz force (2.6 Gz at 1.5m radius) than are water snakes. The tolerance differences of the two species reflected cardiovascular responses, which differed quantitatively but not qualitatively: heart rates increased while arterial pressure and blood flow decreased in response to increasing levels of Gz. Thus, in both species of snakes, a reduced gradient of Gz force (associated with greater centrifuge radius) significantly decreases the Gz level that can be tolerated.

Headlines... Areva on the way toward centrifugation

International Nuclear Information System (INIS)

Anon.

2004-01-01

The French industrial group Areva, that gathers Cogema and Framatome-ANP, has entered into a partnership with the British nuclear consortium Urenco for creating ETC (enrichment technology company) in order to replace its uranium enrichment facility (Georges-Besse-I) that is planned to close in 2012 by a new one (George-Besse-II) that will enter into service as early as 2007. The new facility will be based on the centrifugation technique developed by Urenco, this technique will cut the consumption of electricity by 3 in comparison with the gaseous diffusion technique used in the Georges-Besse-I facility. The other asset of the centrifugation technique is that the facility can grow with the number of centrifuges that are set. In 2007 only 7% of the total number of centrifuges will be installed, which will sufficient to satisfy the demand for enriched uranium. The full size of the facility will be reached in 2016 through gradual steps of 10% more centrifuges set every year. (A.C.)

Plasma centrifugation does not influence thrombin-antithrombin and plasmin-antiplasmin levels but determines platelet microparticles count.

Science.gov (United States)

Stępień, Ewa; Gruszczyński, Krzysztof; Kapusta, Przemysław; Kowalik, Artur; Wybrańska, Iwona

2015-01-01

Centrifugation is an essential step for plasma preparation to remove residual elements in plasma, especially platelets and platelet-derived microparticles (PMPs). Our working hypothesis was that centrifugation as a preanalytical step may influence some coagulation parameters. Healthy young men were recruited (N=17). For centrifugation, two protocols were applied: (A) the first centrifugation at 2500xg for 15 min and (B) at 2500xg for 20 min at room temperature with a light brake. In protocol (A), the second centrifugation was carried out at 2500xg for 15 min, whereas in protocol (B), the second centrifugation involved a 10 min spin at 13,000 x g. Thrombin-antithrombin (TAT) and plasmin-antiplasmin (PAP) complexes concentrations were determined by enzyme-linked immunosorbent assays. PMPs were stained with CD41 antibody and annexin V, and analyzed by flow cytometry method. Procoagulant activity was assayed by the Calibrated Automated Thrombogram method as a slope of thrombin formation (CAT velocity). Median TAT and PAP concentrations did not differ between the centrifugation protocols. The high speed centrifugation reduced the median (IQR) PMP count in plasma from 1291 (841-1975) to 573 (391-1010) PMP/µL (P=0.001), and CAT velocity from 2.01 (1.31-2.88) to 0.97 (0.82-1.73) nM/min (P=0.049). Spearman's rank correlation analysis showed correlation between TAT and PMPs in the protocol A plasma which was (rho=0.52, PCentrifugation protocols do not influence the markers of plasminogen (PAP) and thrombin (TAT) generation but they do affect the PMP count and procoagulant activity.

Efficient two-level preconditionined conjugate gradient method on the GPU

NARCIS (Netherlands)

Gupta, R.; Van Gijzen, M.B.; Vuik, K.

2011-01-01

We present an implementation of Two-Level Preconditioned Conjugate Gradient Method for the GPU. We investigate a Truncated Neumann Series based preconditioner in combination with deflation and compare it with Block Incomplete Cholesky schemes. This combination exhibits fine-grain parallelism and

A numerically research on energy loss evaluation in a centrifugal pump system based on local entropy production method

Directory of Open Access Journals (Sweden)

Hou Hucan

2017-01-01

Full Text Available Inspired by wide application of the second law of thermodynamics to flow and heat transfer devices, local entropy production analysis method was creatively introduced into energy assessment system of centrifugal water pump. Based on Reynolds stress turbulent model and energy equation model, the steady numerical simulation of the whole flow passage of one IS centrifugal pump was carried out. The local entropy production terms were calculated by user defined functions, mainly including wall entropy production, turbulent entropy production, and viscous entropy production. The numerical results indicated that the irreversible energy loss calculated by the local entropy production method agreed well with that calculated by the traditional method but with some deviations which were probably caused by high rotatability and high curvature of impeller and volute. The wall entropy production and turbulent entropy production took up large part of the whole entropy production about 48.61% and 47.91%, respectively, which indicated that wall friction and turbulent fluctuation were the major factors in affecting irreversible energy loss. Meanwhile, the entropy production rate distribution was discussed and compared with turbulent kinetic energy dissipation rate distribution, it showed that turbulent entropy production rate increased sharply at the near wall regions and both distributed more uniformly. The blade region in leading edge near suction side, trailing edge and volute tongue were the main regions to generate irreversible exergy loss. This research broadens a completely new view in evaluating energy loss and further optimizes pump using entropy production minimization.

Experimental Investigation on Centrifugal Compressor Blade Crack Classification Using the Squared Envelope Spectrum

Directory of Open Access Journals (Sweden)

Xuefeng Zhang

2013-09-01

Full Text Available Centrifugal compressors are a key piece of equipment for modern production. Among the components of the centrifugal compressor, the impeller is a pivotal part as it is used to transform kinetic energy into pressure energy. Blade crack condition monitoring and classification has been broadly investigated in the industrial and academic area. In this research, a pressure pulsation (PP sensor arranged in close vicinity to the crack area and the corresponding casing vibration signals are used to monitor blade crack information. As these signals cannot directly demonstrate the blade crack, the method employed in this research is based on the extraction of weak signal characteristics that are induced by blade cracking. A method for blade crack classification based on the signals monitored by using a squared envelope spectrum (SES is presented. Experimental investigations on blade crack classification are carried out to verify the effectiveness of this method. The results show that it is an effective tool for blade crack classification in centrifugal compressors.

Experimental investigation on centrifugal compressor blade crack classification using the squared envelope spectrum.

Science.gov (United States)

Li, Hongkun; Zhang, Xuefeng; Xu, Fujian

2013-09-18

Centrifugal compressors are a key piece of equipment for modern production. Among the components of the centrifugal compressor, the impeller is a pivotal part as it is used to transform kinetic energy into pressure energy. Blade crack condition monitoring and classification has been broadly investigated in the industrial and academic area. In this research, a pressure pulsation (PP) sensor arranged in close vicinity to the crack area and the corresponding casing vibration signals are used to monitor blade crack information. As these signals cannot directly demonstrate the blade crack, the method employed in this research is based on the extraction of weak signal characteristics that are induced by blade cracking. A method for blade crack classification based on the signals monitored by using a squared envelope spectrum (SES) is presented. Experimental investigations on blade crack classification are carried out to verify the effectiveness of this method. The results show that it is an effective tool for blade crack classification in centrifugal compressors.

Performance and internal flow condition of mini centrifugal pump with splitter blades

International Nuclear Information System (INIS)

Shigemitsu, T; Fukutomi, J; Kaji, K; Wada, T

2012-01-01

Mini centrifugal pumps having a diameter smaller than 100mm are employed in many fields. But the design method for the mini centrifugal pump is not established because the internal flow condition for these small-sized fluid machines is not clarified and conventional theory is not suitable for small-sized pumps. Therefore, mini centrifugal pumps with simple structure were investigated by this research. Splitter blades were adopted in this research to improve the performance and the internal flow condition of mini centrifugal pump which had large blade outlet angle. The original impeller without the splitter blades and the impeller with the splitter blades were prepared for an experiment. The performance tests are conducted with these rotors in order to investigate the effect of the splitter blades on performance and internal flow condition of mini centrifugal pump. On the other hand, a three dimensional steady numerical flow analysis is conducted with the commercial code (ANSYS-CFX) to investigate the internal flow condition in detail. It is clarified from the experimental results that the performance of the mini centrifugal pump is improved by the effect of the splitter blades. The blade-to-blade low velocity regions are suppressed in the case with the splitter blades and the total pressure loss regions are decreased. The effects of the splitter blades on the performance and the internal flow condition are discussed in this paper.

Wave-driven countercurrent plasma centrifuge

Energy Technology Data Exchange (ETDEWEB)

Fetterman, Abraham J; Fisch, Nathaniel J [Department of Astrophysical Sciences, Princeton University, Princeton, NJ 08540 (United States)

2009-11-15

A method for driving rotation and a countercurrent flow in a fully ionized plasma centrifuge is described. The rotation is produced by radiofrequency waves near the cyclotron resonance. The wave energy is transferred into potential energy in a manner similar to the {alpha} channeling effect. The countercurrent flow may also be driven by radiofrequency waves. By driving both the rotation and the flow pattern using waves instead of electrodes, physical and engineering issues may be avoided.

Wave-driven countercurrent plasma centrifuge

International Nuclear Information System (INIS)

Fetterman, Abraham J; Fisch, Nathaniel J

2009-01-01

A method for driving rotation and a countercurrent flow in a fully ionized plasma centrifuge is described. The rotation is produced by radiofrequency waves near the cyclotron resonance. The wave energy is transferred into potential energy in a manner similar to the α channeling effect. The countercurrent flow may also be driven by radiofrequency waves. By driving both the rotation and the flow pattern using waves instead of electrodes, physical and engineering issues may be avoided.

Wave-driven Countercurrent Plasma Centrifuge

International Nuclear Information System (INIS)

Fetterman, A.J.; Fisch, N.J.

2009-01-01

A method for driving rotation and a countercurrent flow in a fully ionized plasma centrifuge is described. The rotation is produced by radiofrequency waves near the cyclotron resonance. The wave energy is transferred into potential energy in a manner similar to the α channeling effect. The countercurrent flow may also be driven by radiofrequency waves. By driving both the rotation and the flow pattern using waves instead of electrodes, physical and engineering issues may be avoided

Two-level preconditioned conjugate gradient methods with applications to bubbly flow problems

NARCIS (Netherlands)

Tang, J.M.

2008-01-01

The Preconditioned Conjugate Gradient (PCG) method is one of the most popular iterative methods for solving large linear systems with a symmetric and positive semi-definite coefficient matrix. However, if the preconditioned coefficient matrix is ill-conditioned, the convergence of the PCG method

Laser-based data acquisition in gas centrifuge environments using optical fibers

International Nuclear Information System (INIS)

Cates, M.R.; Allison, S.W.; Marshall, B.; Davies, T.J.; Franks, L.A.; Nelson, M.A.; Noel, B.W.

1984-01-01

The operating environment of gas centrifuges poses three basic experimental problems: rotating reference frame, corrosive effects of UF 6 gas, and vacuum coupling. Diagnostic experiments in this environment effectively use fiber optics as laser transport systems and data extraction channels. Access to the interior of rotating centrifuges is only from a central nonrotating column assembly. Optical paths are often long and difficult to measure in static conditions with precision necessary in operating conditions. Residual traces of HF gas, from UF 6 , damage exposed optical components over time. Diagnostic measurements requiring pulsed laser sources and analysis of fluorescence emissions, both from UF 6 gas and from temperature-sensitive phosphor are described, with emphasis on optical fiber components and experimental design configurations. The studies were done at Oak Ridge Gaseous Diffusion Plant through the Centrifuge Physics Department of the Centrifuge Division. The advantages of fiber optics methods include: optical path flexibility, small and adaptable size of components, utility in connection with moveable assemblies, and relative ease of vacuum isolation. 3 references, 6 figures

A projection gradient method for computing ground state of spin-2 Bose–Einstein condensates

Energy Technology Data Exchange (ETDEWEB)

Wang, Hanquan, E-mail: hanquan.wang@gmail.com [School of Statistics and Mathematics, Yunnan University of Finance and Economics, Kunming, Yunnan Province, 650221 (China); Yunnan Tongchang Scientific Computing and Data Mining Research Center, Kunming, Yunnan Province, 650221 (China)

2014-10-01

In this paper, a projection gradient method is presented for computing ground state of spin-2 Bose–Einstein condensates (BEC). We first propose the general projection gradient method for solving energy functional minimization problem under multiple constraints, in which the energy functional takes real functions as independent variables. We next extend the method to solve a similar problem, where the energy functional now takes complex functions as independent variables. We finally employ the method into finding the ground state of spin-2 BEC. The key of our method is: by constructing continuous gradient flows (CGFs), the ground state of spin-2 BEC can be computed as the steady state solution of such CGFs. We discretized the CGFs by a conservative finite difference method along with a proper way to deal with the nonlinear terms. We show that the numerical discretization is normalization and magnetization conservative and energy diminishing. Numerical results of the ground state and their energy of spin-2 BEC are reported to demonstrate the effectiveness of the numerical method.

A projection gradient method for computing ground state of spin-2 Bose–Einstein condensates

International Nuclear Information System (INIS)

Wang, Hanquan

2014-01-01

In this paper, a projection gradient method is presented for computing ground state of spin-2 Bose–Einstein condensates (BEC). We first propose the general projection gradient method for solving energy functional minimization problem under multiple constraints, in which the energy functional takes real functions as independent variables. We next extend the method to solve a similar problem, where the energy functional now takes complex functions as independent variables. We finally employ the method into finding the ground state of spin-2 BEC. The key of our method is: by constructing continuous gradient flows (CGFs), the ground state of spin-2 BEC can be computed as the steady state solution of such CGFs. We discretized the CGFs by a conservative finite difference method along with a proper way to deal with the nonlinear terms. We show that the numerical discretization is normalization and magnetization conservative and energy diminishing. Numerical results of the ground state and their energy of spin-2 BEC are reported to demonstrate the effectiveness of the numerical method

The centrifugal sudden distorted wave method for chemical reactions: Application to Cl+HCl → ClH+Cl

International Nuclear Information System (INIS)

Amaee, B.; Connor, J.N.L.; Schatz, G.C.

1987-01-01

The authors describe a method for calculating cross sections for atom plus diatom reactive collisions based on the centrifugal sudden distorted wave (CSDW) approximation. This method is nearly exact at low energies where reactive cross sections are small. Representative CPU times are given for applications of CSDW method to the Cl + HCl → ClH + Cl reaction using CDC 7600, Cyber 176, Cyber 205, Cray XMP and Cray 2 computers. Calculations show that the product HCl molecule is highly rotationally excited, (receiving 40-50% of the available energy) and that the shape of the product rotational distribution is nearly independent of reagent rotational state. The authors also calculated product differential cross sections and find them to be backward peaked at low energies

Separative power of an optimised concurrent gas centrifuge

Energy Technology Data Exchange (ETDEWEB)

Bogovalov, Sergey; Boman, Vladimir [National Research Nuclear University (MEPHI), Moscow (Russian Federation)

2016-06-15

The problem of separation of isotopes in a concurrent gas centrifuge is solved analytically for an arbitrary binary mixture of isotopes. The separative power of the optimised concurrent gas centrifuges for the uranium isotopes equals to δU = 12.7 (V/700 m/s)2(300 K/T)(L/1 m) kg·SWU/yr, where L and V are the length and linear velocity of the rotor of the gas centrifuge and T is the temperature. This equation agrees well with the empirically determined separative power of optimised counter-current gas centrifuges.

Dynamics and stability of a tethered centrifuge in low earth orbit

Science.gov (United States)

Quadrelli, B. M.; Lorenzini, E. C.

1992-01-01

The three-dimensional attitude dynamics of a spaceborne tethered centrifuge for artificial gravity experiments in low earth orbit is analyzed using two different methods. First, the tethered centrifuge is modeled as a dumbbell with a straight viscoelastic tether, point tip-masses, and sophisticated environmental models such as nonspherical gravity, thermal perturbations, and a dynamic atmospheric model. The motion of the centrifuge during spin-up, de-spin, and steady-rotation is then simulated. Second, a continuum model of the tether is developed for analyzing the stability of lateral tether oscillations. Results indicate that the maximum fluctuation about the 1-g radial acceleration level is less than 0.001 g; the time required for spin-up and de-spin is less than one orbit; and lateral oscillations are stable for any practical values of the system parameters.

Review on stress corrosion and corrosion fatigue failure of centrifugal compressor impeller

Science.gov (United States)

Sun, Jiao; Chen, Songying; Qu, Yanpeng; Li, Jianfeng

2015-03-01

Corrosion failure, especially stress corrosion cracking and corrosion fatigue, is the main cause of centrifugal compressor impeller failure. And it is concealed and destructive. This paper summarizes the main theories of stress corrosion cracking and corrosion fatigue and its latest developments, and it also points out that existing stress corrosion cracking theories can be reduced to the anodic dissolution (AD), the hydrogen-induced cracking (HIC), and the combined AD and HIC mechanisms. The corrosion behavior and the mechanism of corrosion fatigue in the crack propagation stage are similar to stress corrosion cracking. The effects of stress ratio, loading frequency, and corrosive medium on the corrosion fatigue crack propagation rate are analyzed and summarized. The corrosion behavior and the mechanism of stress corrosion cracking and corrosion fatigue in corrosive environments, which contain sulfide, chlorides, and carbonate, are analyzed. The working environments of the centrifugal compressor impeller show the behavior and the mechanism of stress corrosion cracking and corrosion fatigue in different corrosive environments. The current research methods for centrifugal compressor impeller corrosion failure are analyzed. Physical analysis, numerical simulation, and the fluid-structure interaction method play an increasingly important role in the research on impeller deformation and stress distribution caused by the joint action of aerodynamic load and centrifugal load.

Autobalancing and FDIR for a space-based centrifuge prototype

Science.gov (United States)

Wilson, Edward; Mah, Robert W.

2005-01-01

This report summarizes centrifuge-related work performed at the Smart Systems Research Laboratory at NASA Ames Research Center's Computational Sciences Division from 1995 through 2003. The goal is to develop an automated system that will sense an imbalance (both static and dynamic3) in a centrifuge and issue control commands to drive counterweights to eliminate the effects of the imbalance. This autobalancing development began when the ISS centrifuge design was not yet finalized, and was designed to work with the SSRL Centrifuge laboratory prototype, constructed in 1993-1995. Significant differences between that prototype and the current International Space Station (ISS) Centrifuge design are that: the spin axis for the SSRL Centrifuge prototype can translate freely in x and y, but not wobble, whereas the ISS centrifuge spin axis has 3 translational and two rotational degrees of freedom, supported by a vibration 34. The imbalance sensors are strained gauges both in the rotor and the stator, measuring the imbalance forces, whereas the ISS centrifuge uses eddy current displacement sensors to measure the displacements resulting from imbalance. High fidelity autobalancing and FDIR systems (for both counterweights and strain gauges) are developed and tested in MATLAB simulation, for the SSRL Centrifuge configuration. Hardware implementation of the autobalancing technology was begun in 1996, but was terminated due to lack of funding. The project lay dormant until 2001-2002 when the FDIR capability was added.

High frequency dynamics in centrifugal compressors

NARCIS (Netherlands)

Twerda, A.; Meulendijks, D.; Smeulers, J.P.M.; Handel, R. van den; Lier, L.J. van

2008-01-01

Problems with centrifugal compressors relating to high frequency, i.e. Blade passing frequency (BPF) are increasing. Pulsations and vibrations generated in centrifugal compressors can lead to nuisance, due to strong tonal noise, and even breakdown. In several cases the root cause of a failure or a

Thermal analysis of a gas centrifuge

International Nuclear Information System (INIS)

Andrade, D.A.; Bastos, J.L.F.; Maiorino, J.R.

1996-01-01

The centrifuge separation efficiency is the result of the composition of the centrifuge field to the secondary flow in the axial direction near to the rotor wall. For a given machine, the centrifuge field can not be altered and the effort to augment the separation efficiency should be concentrated on the secondary flow. The secondary flow has a mechanical and a thermal component. The mechanical component is due to the deceleration of the gas at the scoop region. The thermal component is due to the temperature differences at the rotor. This paper presents a thermal model of a centrifuge in order to understand the main heat transfer mechanisms and to establish the boundary conditions for a fluid flow computer code. The heat transfer analysis takes into account conduction at the structure parts of the rotor and shell, radiation with multi-reflections between the rotor and the shell, and convection to the ambient. (author)

Centrifugal trapping in the magnetotail

Directory of Open Access Journals (Sweden)

D. C. Delcourt

1995-03-01

Full Text Available Particles leaving the neutral sheet in the distant magnetotail at times display adiabatic trajectory sequences characterized by an inflection toward the equator and subsequent mirroring in its vicinity. We demonstrate that this low-latitude mirroring results primarily from a centrifugal deceleration due to the fast direction-changing E×B drift. This effect which we refer to as "centrifugal trapping" appears both in guiding centre and full particle treatments. It thus does not directly relate to nonadiabatic motion. However, pitch angle scattering due to nonadiabatic neutral sheet interaction does play a role in reducing the parallel speed of the particles. We show that centrifugal trapping is an important mechanism for the confinement of the slowest (typically below the equatorial E×B drift speed plasma sheet populations to the midplane vicinity.

Accelerated gradient methods for total-variation-based CT image reconstruction

DEFF Research Database (Denmark)

Jørgensen, Jakob Heide; Jensen, Tobias Lindstrøm; Hansen, Per Christian

2011-01-01

incorporates several heuristics from the optimization literature such as Barzilai-Borwein (BB) step size selection and nonmonotone line search. The latter uses a cleverly chosen sequence of auxiliary points to achieve a better convergence rate. The methods are memory efficient and equipped with a stopping...... reconstruction can in principle be found by any optimization method, but in practice the large scale of the systems arising in CT image reconstruction preclude the use of memory-demanding methods such as Newton’s method. The simple gradient method has much lower memory requirements, but exhibits slow convergence...

Safety aspects of gas centrifuge enrichment plants

International Nuclear Information System (INIS)

Hansen, A.H.

1987-01-01

Uranium enrichment by gas centrifuge is a commercially proven, viable technology. Gas centrifuge enrichment plant operations pose hazards that are also found in other industries as well as unique hazards as a result of processing and handling uranium hexafluoride and the handling of enriched uranium. Hazards also found in other industries included those posed by the use of high-speed rotating equipment and equipment handling by use of heavy-duty cranes. Hazards from high-speed rotating equipment are associated with the operation of the gas centrifuges themselves and with the operation of the uranium hexafluoride compressors in the tail withdrawal system. These and related hazards are discussed. It is included that commercial gas centrifuge enrichment plants have been designed to operate safely

On the solution of the Hartree-Fock-Bogoliubov equations by the conjugate gradient method

International Nuclear Information System (INIS)

Egido, J.L.; Robledo, L.M.

1995-01-01

The conjugate gradient method is formulated in the Hilbert space for density and non-density dependent Hamiltonians. We apply it to the solution of the Hartree-Fock-Bogoliubov equations with constraints. As a numerical application we show calculations with the finite range density dependent Gogny force. The number of iterations required to reach convergence is reduced by a factor of three to four as compared with the standard gradient method. (orig.)

Centrifugal Casting Features/Metallurgical Characterization of Aluminum Alloys

International Nuclear Information System (INIS)

Chirita, G.; Soares, D.; Cruz, D.; Silva, F. S.; Stefanescu, I.

2008-01-01

This paper deals with the study of centrifugal effects on aluminium castings under high G values. Most of the studies in this domain (FGMs obtained by centrifugal casting) deal with functionally graded composites reinforced with a solid phase such as silicon particles or others. However, in this study it will be shown that unreinforced aluminium alloys may be significantly influenced by the centrifugal effect and that functionally graded castings are also obtained. It has been observed that the centrifugal effect may increase in some alloys, depending on the relative position in the castings, the rupture strength by approx. 50%, and rupture strain by about 300%, as compared to the gravity casting technique. The Young's modulus may also increase by about 20%. It has also been reported that in vertical centrifugal castings there are mainly three aspects that affect the components thus obtained, namely: fluid dynamics; vibration (inherent to the system); and centrifugal force. These features have a different effect on the castings depending on the aluminium alloy. In this paper, an analysis of the most important effects of the centrifugal casting process on metallurgical features is conducted. A solidification characterization at several points along the mould will be made in order to have an accurate idea of both the fluid dynamics inside the mould during the casting and the solidification behavior in different parts of the component. These two analyses will be related to the metallurgical properties (phase distribution; SDAS; eutectic silicon content and shape, pores density and shape) along the component and mainly along the direction of the centrifugal pressure. A comparison between castings obtained by both centrifugal casting technique and gravity casting technique is made for reference (gravity casting)

Improved g-level calculations for coil planet centrifuges.

Science.gov (United States)

van den Heuvel, Remco N A M; König, Carola S

2011-09-09

Calculation of the g-level is often used to compare CCC centrifuges, either against each other or to allow for comparison with other centrifugal techniques. This study shows the limitations of calculating the g-level in the traditional way. Traditional g-level calculations produce a constant value which does not accurately reflect the dynamics of the coil planet centrifuge. This work has led to a new equation which can be used to determine the improved non-dimensional values. The new equations describe the fluctuating radial and tangential g-level associated with CCC centrifuges and the mean radial g-level value. The latter has been found to be significantly different than that determined by the traditional equation. This new equation will give a better understanding of forces experienced by sample components and allows for more accurate comparison between centrifuges. Although the new equation is far better than the traditional equation for comparing different types of centrifuges, other factors such as the mixing regime may need to be considered to improve the comparison further. Copyright © 2011 Elsevier B.V. All rights reserved.

Why have we stopped research on liquid centrifugal separation

International Nuclear Information System (INIS)

Li, N.

1996-01-01

Using high-temperature high-speed liquid centrifuges for lanthanides and actinides separation was originally proposed as a physical separation method in the Los Alamos ADTT/ATW concept [C. Bowman, LA-UR-92-1065 (1992)]. The authors investigated centrifugal separation in a concerted effort of experiments, theoretical analysis and numerical simulations. They discovered that owing to the ionic-composition-dependence of the sedimentation coefficients for the fission products and actinides, separation by grouping of molecular densities would not work in general in the molten salt environment. Alternatively the lanthanides and actinides could be transferred to a liquid metal carrier (e.g. bismuth) via reductive extraction and then separated by liquid centrifuges, but the material and technical challenges are severe. Meanwhile the authors have established that the reductive extraction procedure itself can be used for desired separations. Unlike conventional aqueous-based reprocessing technologies, reductive extraction separation uses only reagent (Li) that reconstitutes carrier salts (LiF-BeF 2 ) and a processing medium (Bi) that can be continuously recycled and reused, with a nearly-pure fission products waste stream. The processing units are compact and reliable, and can be built at relatively low cost while maintaining high throughput. Therefore the research effort on developing liquid centrifuges for separations in ADTT/ATW was terminated in late 1995. This paper will discuss the various aspects involved in reaching this decision

Analysis and performance estimation of the Conjugate Gradient method on multiple GPUs

NARCIS (Netherlands)

Verschoor, M.; Jalba, A.C.

2012-01-01

The Conjugate Gradient (CG) method is a widely-used iterative method for solving linear systems described by a (sparse) matrix. The method requires a large amount of Sparse-Matrix Vector (SpMV) multiplications, vector reductions and other vector operations to be performed. We present a number of

Proposal of method for quantifying uranium weight in the interior of gas centrifuge, and performance evaluation of IF7 treatment reaction product separation and refinement

International Nuclear Information System (INIS)

Ema, Akira; Kado, Kazumi; Suzuki, Kazuhiko

2011-01-01

In uranium enrichment plant decommissioning, it is important to treat and dispose the gas centrifuge reasonably. At the Ningyo-Toge Environmental Engineering Center of JAEA, it has been planned to carry out IF 7 treatment for the gas centrifuge in the uranium enrichment plant for decontamination before dismantling. In order to carry out this IF 7 treatment, a nondestructive measurement technology to estimate the uranium weight in the interior of the gas centrifuge was needed. However, it was difficult to estimate exactly uranium weight using former methods. Therefore, in this study, we investigated a new nondestructive measurement. As a result, we succeeded in constructing theoretically the methodology using γ-ray intensity measured outside the gas centrifuge and MCNP code. Furthermore, the UF 6 and IF 5 mixture was recovered by IF 7 treatment. A technology to separate and refine IF 5 from this mixture was needed in order to use as a material of IF 7 gas. Therefore, in this study, we carried out IF 5 separation and refinement examination. This paper shows the investigation result of reasonable separation condition and the full-scale examination result. As a result, it could be proved that 86% of IF 5 was separated and refined from UF 6 and IF 5 mixture. (author)

[The analytical setting of rotary speed of centrifuge rotor and centrifugation time in chemical, biochemical and microbiological practice].

Science.gov (United States)

Zolotarev, K V

2012-08-01

The researchers happen to face with suspensions in their chemical, biochemical and microbiological practice. The suspensions are the disperse systems with solid dispersed phase and liquid dispersion medium and with dispersed phase particle size > 100 nm (10-7 m). Quite often the necessity occurs to separate solid particles from liquid. To use for this purpose the precipitation in gravitation field can make the process to progress too long. In this respect an effective mode is the precipitation in the field of centrifugal forces--the centrifugation. The rotary speed of centrifuge rotor and centrifugation time can be set analytically using regularities of general dynamics and hydrodynamics. To this effect, should be written and transformed the equation of First and Second Newton Laws for suspension particle being in the field of centrifugal forces and forces of resistance of liquid and vessel wall. The force of liquid resistance depends on particle motion condition in liquid. To determine the regimen the Archimedes and Reynolds numerical dimensionless criteria are to be applied. The article demonstrates the results of these transformations as analytical inverse ratio dependence of centrifugation time from rotary speed. The calculation of series of "rate-time" data permits to choose the optimal data pair on the assumption of centrifuge capacity and practical reasonability. The results of calculations are validated by actual experimental data hence the physical mathematical apparatus can be considered as effective one. The setting progress depends both from parameter (Reynolds criterion) and data series calculation. So, the most convenient way to apply this operation is the programming approach. The article proposes to use the program Microsoft Excel and VBA programming language for this purpose. The possibility to download the file from Internet to use it for fast solution is proposed.

Achieving an ever-improving centrifuge

International Nuclear Information System (INIS)

Edwards, T.T.; Wilcox, P.

1988-01-01

To ensure that the latest technical innovations can be rapidly incorporated, centrifuge development in the Urenco organization is carried out in different phases simultaneously on different generations of machines. This system has led to progressively increased outputs and reduced specific costs, and with the further known potential available, is expected to maintain Urenco's competitiveness throughout the 1990s. The process of separating isotopes by centrifuge is described. (author)

Water isotope partitioning and ecohydrologic separation in mixed conifer forest explored with a centrifugation water extraction method

Science.gov (United States)

Bowers, W.; Mercer, J.; Pleasants, M.; Williams, D. G.

2017-12-01

Isotopic partitioning of water within soil into tightly and loosely bound fractions has been proposed to explain differences between isotopic water sources used by plants and those that contribute to streams and ground water, the basis for the "two water worlds" hypothesis. We examined the isotope ratio values of water in trees, bulk soil, mobile water collected from soil lysimeters, stream water, and GW at three different hillslopes in a mixed conifer forest in southeastern Wyoming, USA. Hillslopes differed in aspect and topographic position with corresponding differences in surface energy balance, snowmelt timing, and duration of soil moisture during the dry summer. The isotopic results support the partitioning of water within the soil; trees apparently used a different pool of water for transpiration than that recovered from soil lysimeters and the source was not resolved with the isotopic signature of the water that was extracted from bulk soil via cryogenic vacuum distillation. Separating and measuring the isotope ratios values in these pools would test the assumption that the tightly bound water within the soil has the same isotopic signature as the water transpired by the trees. We employed a centrifugation approach to separate water within the soil held at different tensions by applying stepwise increases in rotational velocity and pressures to the bulk soil samples. Effluent and the remaining water (cryogenically extracted) at each step were compared. We first applied the centrifugation method in a simple lab experiment using sandy loam soil and separate introductions of two isotopically distinct waters. We then applied the method to soil collected from the montane hillslopes. For the lab experiment, we predicted that effluents would have distinct isotopic signatures, with the last effluent and extracted water more closely representing the isotopic signature of the first water applied. For our field samples, we predicted that the isotopic signature of the

Numerical investigation of a new LH2 centrifugal pump concept used in space propulsion

Directory of Open Access Journals (Sweden)

Ion MALAEL

2018-06-01

Full Text Available The present study deals with efficiency increase of a centrifugal pump for liquid rocket propulsion by using an innovative concept. With this new pump design the axial length will be reduced by 60% and 20% mass weight towards a classic two stage centrifugal pump. To estimate the performances, the CFD methods were used. The CFD analysis will be performed on 3D domains with the CFD commercial code ANSYS CFX. The numerical solvers used are pressure based with the SIMPLE method for RANS. The domain discretization was done by using dedicated grid generators like TurboGrid and ICEM CFD. The results were compared with a classic configuration with two stages in series. Centrifugal pump characteristics, such as pressure inlet-outlet variation, velocity and streamline patterns are presented in the paper.

Rhie-Chow interpolation in strong centrifugal fields

Science.gov (United States)

Bogovalov, S. V.; Tronin, I. V.

2015-10-01

Rhie-Chow interpolation formulas are derived from the Navier-Stokes and continuity equations. These formulas are generalized to gas dynamics in strong centrifugal fields (as high as 106 g) occurring in gas centrifuges.

Centrifuge modelling of offshore monopile foundation

DEFF Research Database (Denmark)

Klinkvort, Rasmus Tofte; Hededal, Ole

2010-01-01

centrifuge tests on a laterally loaded monopile in dry sand. The prototype dimension of the piles was modelled to a diameter of 1 meter and penetration depth on 6 meter. The test series were designed in order to investigate the scaling laws in the centrifuge both for monotonic and cyclic loading...

Deflation in preconditioned conjugate gradient methods for Finite Element Problems

NARCIS (Netherlands)

Vermolen, F.J.; Vuik, C.; Segal, A.

2002-01-01

We investigate the influence of the value of deflation vectors at interfaces on the rate of convergence of preconditioned conjugate gradient methods applied to a Finite Element discretization for an elliptic equation. Our set-up is a Poisson problem in two dimensions with continuous or discontinuous

Decontamination of nuclear fuels with centrifugal separation

International Nuclear Information System (INIS)

Ning, Li; Camassa, R.; Ecke, R.; Venneri, F.

1995-01-01

The treatment and disposal of nuclear material is a crucial element in today's nuclear power industry. We present a physical process of centrifugal separation that has potential to deal with existing waste and provide opportunities for realizing advanced accelerator-driven power generation. In our proposed process a liquid metal solution containing actinides and fission products is fed through a series of continuous flow centrifuges. We show fundamentals of centrifugation including theory and experiments and estimate how the processing can be optimized

Decontamination of nuclear fuels with centrifugal separation

International Nuclear Information System (INIS)

Ning, L.; Camassa, R.; Ecke, R.; Venneri, F.

1995-01-01

The treatment and disposal of nuclear material is a crucial element in today's nuclear power industry. We present a physical process of centrifugal separation that has potential to deal with existing waste and provide opportunities for realizing advanced accelerator driven power generation. In our proposed process a liquid metal solution containing actinides and fission products is fed through a series of continuous flow centrifuges. We show fundamentals of centrifugation including theory and experiments and estimate how the processing can be optimized. (authors)

Experimental study of multi-component separation by gas centrifuge

International Nuclear Information System (INIS)

Zhou, M.S.; Liang, X.W.; Chen, W.N.; Yin, Y.T.

2006-01-01

Stable isotopes are applied in many areas and most stable isotopes are multi-component, This paper presents experimental results of several stable isotopes separation conducted in Tsinghua University by using ultra-speed gas centrifuges. Xe, WF 6 , TeF 6 , SiHCl 3 , SiF 4 were chosen as the process gases. By adjusting some of the centrifuge's parameters, the suitable centrifuge parameters for different process gas separations were found and the overall unit separation factors γ 0 were obtained by means of single gas centrifuge separation. The experimental results show that with appropriate process gases, stable isotope separation by gas centrifuge was effective. (authors)

Gradient Calculation Methods on Arbitrary Polyhedral Unstructured Meshes for Cell-Centered CFD Solvers

Science.gov (United States)

Sozer, Emre; Brehm, Christoph; Kiris, Cetin C.

2014-01-01

A survey of gradient reconstruction methods for cell-centered data on unstructured meshes is conducted within the scope of accuracy assessment. Formal order of accuracy, as well as error magnitudes for each of the studied methods, are evaluated on a complex mesh of various cell types through consecutive local scaling of an analytical test function. The tests highlighted several gradient operator choices that can consistently achieve 1st order accuracy regardless of cell type and shape. The tests further offered error comparisons for given cell types, leading to the observation that the "ideal" gradient operator choice is not universal. Practical implications of the results are explored via CFD solutions of a 2D inviscid standing vortex, portraying the discretization error properties. A relatively naive, yet largely unexplored, approach of local curvilinear stencil transformation exhibited surprisingly favorable properties

Extension of Modified Polak-Ribière-Polyak Conjugate Gradient Method to Linear Equality Constraints Minimization Problems

Directory of Open Access Journals (Sweden)

Zhifeng Dai

2014-01-01

Full Text Available Combining the Rosen gradient projection method with the two-term Polak-Ribière-Polyak (PRP conjugate gradient method, we propose a two-term Polak-Ribière-Polyak (PRP conjugate gradient projection method for solving linear equality constraints optimization problems. The proposed method possesses some attractive properties: (1 search direction generated by the proposed method is a feasible descent direction; consequently the generated iterates are feasible points; (2 the sequences of function are decreasing. Under some mild conditions, we show that it is globally convergent with Armijio-type line search. Preliminary numerical results show that the proposed method is promising.

Axial and Centrifugal Compressor Mean Line Flow Analysis Method

Science.gov (United States)

Veres, Joseph P.

2009-01-01

This paper describes a method to estimate key aerodynamic parameters of single and multistage axial and centrifugal compressors. This mean-line compressor code COMDES provides the capability of sizing single and multistage compressors quickly during the conceptual design process. Based on the compressible fluid flow equations and the Euler equation, the code can estimate rotor inlet and exit blade angles when run in the design mode. The design point rotor efficiency and stator losses are inputs to the code, and are modeled at off design. When run in the off-design analysis mode, it can be used to generate performance maps based on simple models for losses due to rotor incidence and inlet guide vane reset angle. The code can provide an improved understanding of basic aerodynamic parameters such as diffusion factor, loading levels and incidence, when matching multistage compressor blade rows at design and at part-speed operation. Rotor loading levels and relative velocity ratio are correlated to the onset of compressor surge. NASA Stage 37 and the three-stage NASA 74-A axial compressors were analyzed and the results compared to test data. The code has been used to generate the performance map for the NASA 76-B three-stage axial compressor featuring variable geometry. The compressor stages were aerodynamically matched at off-design speeds by adjusting the variable inlet guide vane and variable stator geometry angles to control the rotor diffusion factor and incidence angles.

Centrifugation-free washing: A novel approach for removing immunoglobulin A from stored red blood cells.

Science.gov (United States)

Vörös, Eszter; Piety, Nathaniel Z; Strachan, Briony C; Lu, Madeleine; Shevkoplyas, Sergey S

2018-08-01

Washed red blood cells (RBCs) are indicated for immunoglobulin A (IgA) deficient recipients. Centrifugation-based cell processors commonly used by hospital blood banks cannot consistently reduce IgA below the recommended levels, hence double washing is frequently required. Here, we describe a prototype of a simple, portable, disposable system capable of washing stored RBCs without centrifugation, while reducing IgA below 0.05 mg/dL in a single run. Samples from RBC units (n = 8, leukoreduced, 4-6 weeks storage duration) were diluted with normal saline to a hematocrit of 10%, and then washed using either the prototype washing system, or via conventional centrifugation. The efficiency of the two washing methods was quantified and compared by measuring several key in vitro quality metrics. The prototype of the washing system was able to process stored RBCs at a rate of 300 mL/hour, producing a suspension of washed RBCs with 43 ± 3% hematocrit and 86 ± 7% cell recovery. Overall, the two washing methods performed similarly for most measured parameters, lowering the concentration of free hemoglobin by >4-fold and total free protein by >10-fold. Importantly, the new washing system reduced the IgA level to 0.02 ± 0.01 mg/mL, a concentration 5-fold lower than that produced by conventional centrifugation. This proof-of-concept study showed that centrifugation may be unnecessary for washing stored RBCs. A simple, disposable, centrifugation-free washing system could be particularly useful in smaller medical facilities and resource limited settings that may lack access to centrifugation-based cell processors. © 2017 Wiley Periodicals, Inc.

Centrifugal trapping in the magnetotail

Directory of Open Access Journals (Sweden)

D. C. Delcourt

Full Text Available Particles leaving the neutral sheet in the distant magnetotail at times display adiabatic trajectory sequences characterized by an inflection toward the equator and subsequent mirroring in its vicinity. We demonstrate that this low-latitude mirroring results primarily from a centrifugal deceleration due to the fast direction-changing E×B drift. This effect which we refer to as "centrifugal trapping" appears both in guiding centre and full particle treatments. It thus does not directly relate to nonadiabatic motion. However, pitch angle scattering due to nonadiabatic neutral sheet interaction does play a role in reducing the parallel speed of the particles. We show that centrifugal trapping is an important mechanism for the confinement of the slowest (typically below the equatorial E×B drift speed plasma sheet populations to the midplane vicinity.

Full magnetic gradient tensor from triaxial aeromagnetic gradient measurements: Calculation and application

Science.gov (United States)

Luo, Yao; Wu, Mei-Ping; Wang, Ping; Duan, Shu-Ling; Liu, Hao-Jun; Wang, Jin-Long; An, Zhan-Feng

2015-09-01

The full magnetic gradient tensor (MGT) refers to the spatial change rate of the three field components of the geomagnetic field vector along three mutually orthogonal axes. The tensor is of use to geological mapping, resources exploration, magnetic navigation, and others. However, it is very difficult to measure the full magnetic tensor gradient using existing engineering technology. We present a method to use triaxial aeromagnetic gradient measurements for deriving the full MGT. The method uses the triaxial gradient data and makes full use of the variation of the magnetic anomaly modulus in three dimensions to obtain a self-consistent magnetic tensor gradient. Numerical simulations show that the full MGT data obtained with the proposed method are of high precision and satisfy the requirements of data processing. We selected triaxial aeromagnetic gradient data from the Hebei Province for calculating the full MGT. Data processing shows that using triaxial tensor gradient data allows to take advantage of the spatial rate of change of the total field in three dimensions and suppresses part of the independent noise in the aeromagnetic gradient. The calculated tensor components have improved resolution, and the transformed full tensor gradient satisfies the requirement of geological mapping and interpretation.

Purification of Sarcocystis neurona sporocysts from opossum (Didelphis virginiana) using potassium bromide discontinuous density gradient centrifugation.

Science.gov (United States)

Elsheikha, Hany M; Murphy, Alice J; Fitzgerald, Scott D; Mansfield, Linda S; Massey, Jeffrey P; Saeed, Mahdi A

2003-06-01

This report describes a new, inexpensive procedure for the rapid and efficient purification of Sarcocystis neurona sporocysts from opossum small intestine. S. neurona sporocysts were purified using a discontinuous potassium bromide density gradient. The procedure provides a source of sporocyst wall and sporozoites required for reliable biochemical characterization and for immunological studies directed at characterizing antigens responsible for immunological responses by the host. The examined isolates were identified as S. neurona using random amplified polymorphic DNA primers and restriction endonuclease digestion assays. This method allows the collection of large numbers of highly purified S. neurona sporocysts without loss of sporocyst viability as indicated by propidium iodide permeability and cell culture infectivity assays. In addition, this technique might also be used for sporocyst purification of other Sarcocystis spp.

Advancing haemostasis automation--successful implementation of robotic centrifugation and sample processing in a tertiary service hospital.

Science.gov (United States)

Sédille-Mostafaie, Nazanin; Engler, Hanna; Lutz, Susanne; Korte, Wolfgang

2013-06-01

Laboratories today face increasing pressure to automate operations due to increasing workloads and the need to reduce expenditure. Few studies to date have focussed on the laboratory automation of preanalytical coagulation specimen processing. In the present study, we examined whether a clinical chemistry automation protocol meets the preanalytical requirements for the analyses of coagulation. During the implementation of laboratory automation, we began to operate a pre- and postanalytical automation system. The preanalytical unit processes blood specimens for chemistry, immunology and coagulation by automated specimen processing. As the production of platelet-poor plasma is highly dependent on optimal centrifugation, we examined specimen handling under different centrifugation conditions in order to produce optimal platelet deficient plasma specimens. To this end, manually processed models centrifuged at 1500 g for 5 and 20 min were compared to an automated centrifugation model at 3000 g for 7 min. For analytical assays that are performed frequently enough to be targets for full automation, Passing-Bablok regression analysis showed close agreement between different centrifugation methods, with a correlation coefficient between 0.98 and 0.99 and a bias between -5% and +6%. For seldom performed assays that do not mandate full automation, the Passing-Bablok regression analysis showed acceptable to poor agreement between different centrifugation methods. A full automation solution is suitable and can be recommended for frequent haemostasis testing.

Optimizing platelet-rich plasma gel formation by varying time and gravitational forces during centrifugation.

Science.gov (United States)

Jo, Chris H; Roh, Young Hak; Kim, Ji Eun; Shin, Sue; Yoon, Kang Sup

2013-10-01

Despite the increasing clinical use of topical platelet-rich plasma (PRP) to enhance tissue healing and regeneration, there is no properly standardized method of autologous PRP gel preparation. This study examined the effect of the centrifugation time and gravitational force (g) on the platelet recovery ratio of PRP and determined the most effective centrifugation conditions for preparing PRP. Two-step centrifugations for preparing PRP were used in 39 subjects who had consented prior to the study's start. The separating centrifugation (Step 1, used to separate whole blood into its two main components: red blood cells and plasma) was tested from 500g to 1900g at 200g increments for 5 minutes (min), and from 100g to 1300g at 200g increments for 10 minutes. After separating centrifugation, upper plasma layer was transferred to another plain tube for the condensation centrifugation and remaining lower cell layer was discarded. The condensation centrifugation (Step 2, used to condense the platelets in the separated plasma) was tested at 1000g for 15 min, 1500g for 15 min, 2000g for 5 min and 3000g for 5 min, additionally at 1000g for 10 min and 1500g for 10 min. Platelet gelation was induced by adding 10% calcium gluconate to final PRP with volume ratio of 1:10. The optimal separating centrifugation conditions were followed by 900g for 5 minutes and the condensation conditions were followed by 1500g for 15 minutes, of which recovery ratios were 92.0 ± 3.1% and 84.3 ± 10.0%, respectively.

A nonsmooth nonlinear conjugate gradient method for interactive contact force problems

DEFF Research Database (Denmark)

Silcowitz, Morten; Abel, Sarah Maria Niebe; Erleben, Kenny

2010-01-01

of a nonlinear complementarity problem (NCP), which can be solved using an iterative splitting method, such as the projected Gauss–Seidel (PGS) method. We present a novel method for solving the NCP problem by applying a Fletcher–Reeves type nonlinear nonsmooth conjugate gradient (NNCG) type method. We analyze...... and present experimental convergence behavior and properties of the new method. Our results show that the NNCG method has at least the same convergence rate as PGS, and in many cases better....

HNO3 fluxes to a deciduous forest derived using gradient and REA methods

DEFF Research Database (Denmark)

Pryor, S.C.; Barthelmie, R.J.; Jensen, B.

2002-01-01

Summertime nitric acid concentrations over a deciduous forest in the midwestern United States are reported, which range between 0.36 and 3.3 mug m(-3). Fluxes to the forest are computed using the relaxed eddy accumulation technique and gradient methods. In accord with previous studies, the results...... indicate substantial uncertainties in the gradient-based calculations. The relaxed eddy accumulation (REA) derived fluxes are physically reasonable and are shown to be of similar magnitude to dry deposition estimates from gradient sampling. The REA derived mean deposition velocity is approximately 3 cm s......(-1), which is also comparable to growing season estimates derived by Meyers et al. for a similar deciduous forest. Occasional inverted concentration gradients and fluxes are observed but most are not statistically significant. Data are also presented that indicate substantial through canopy...

Effect of Centrifuge Temperature on Routine Coagulation Tests.

Science.gov (United States)

Yazar, Hayrullah; Özdemir, Fatma; Köse, Elif

2018-01-01

This study investigated the effects of cooled and standard centrifuges on the results of coagulation tests to examine the effects of centrifugation temperature. Equal-volume blood samples from each patient were collected at the same time intervals and subjected to standard (25°C) and cooled centrifugation (2-4°C). Subsequently, the prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (aPTT), fibrinogen, and D-dimer values were determined in runs with the same lot numbers in the same coagulation device using the Dia-PT R (PT and INR), Dia-PTT-liquid (aPTT), Dia-FIB (fibrinogen), and Dia-D-dimer kits, respectively. The study enrolled 771 participants. The PT was significantly (p centrifuges were as follows: PT 10.30 versus 10.50 s; PT (INR) 1.04 versus 1.09 s; APTT 28.90 versus 29.40 s; fibrinogen 321.5 versus 322.1 mg/dL; and D-dimer 179.5 versus 168.7 µg FEU/mL. There were significant differences (p centrifuges. Centrifuge temperature can have a significant effect on the results of coagulation tests. However, broad and specific disease-based studies are needed. © 2018 S. Karger AG, Basel.

Development of uranium enrichment technology by gas centrifugation

International Nuclear Information System (INIS)

Sibata, Tomofumi; Kai, Tsunetoshi

1996-01-01

The development of a gas-centrifuge for uranium enrichment has been conducted by Power Reactor and Nuclear Fuel Development Corporation in Japan after the first several years' fruitless works, the R and D works came to the point and continuing rapid improvements of centrifuges have started, Cascade tests were given with C-1 and C-2 cascade experimental facilities. Life, reliability and feasibility tests were given with the pilot plant and the demonstration plant. As a result of these works, the private commercial plant has started the operation. Although the main efforts were devoted to the development of metal rotor centrifuges in the course mentioned above, composite material rotor centrifuges have also been developed in parallel to achieve higher performance. Promising results have been being obtained with cascade test facilities on the pilot plant scale. Furthermore, R and D works are being proceeded on more excellent and advanced centrifuges. (author)

Isotopic enrichment in a plasma centrifuge

International Nuclear Information System (INIS)

Del Bosco, E.; Dallaqua, R.S.; Ludwig, G.O.; Bittencourt, J.A.

1987-01-01

High rotational velocity and centrifugal isotopic separation of carbon in a vacuum-arc plasma centrifuge are presented. Enrichments of up to 390% for 13 C are measured at 6 cm radius with angular rotation frequencies in excess of 1.0 x 10 5 rad/s in an axial magnetic field of 0.12 T

The use of DNase I, buffer gradient gel, and 35S label for DNA sequencing

International Nuclear Information System (INIS)

Hong, G.F.

1987-01-01

The use of microcentrifuge tubes and mixing of sequencing reactions and brief centrifugation in racks rather than the original capillary tube method has made sequencing reactions relatively simple. Buffer gradient gels and 15 S label are simple means of increasing the rate of sequence analysis; they add little time to that required for determining the sequences of a given number of clones, need no elaborate equipment, and increase the amount of useful data per gel. The standard approach of running 2- and 4-hr gels generates about 300 bases of sequence. The above improvements allow the same number of bases to be read with more confidence from a single 50-cm gel for each clone sequenced due to the changed spacing between sharpened bands

Gas dynamics in strong centrifugal fields

Energy Technology Data Exchange (ETDEWEB)

Bogovalov, S.V.; Kislov, V.A.; Tronin, I.V. [National research nuclear university “MEPhI”, Kashirskoje shosse, 31,115409, Moscow (Russian Federation)

2015-03-10

Dynamics of waves generated by scopes in gas centrifuges (GC) for isotope separation is considered. The centrifugal acceleration in the GC reaches values of the order of 106g. The centrifugal and Coriolis forces modify essentially the conventional sound waves. Three families of the waves with different polarisation and dispersion exist in these conditions. Dynamics of the flow in the model GC Iguasu is investigated numerically. Comparison of the results of the numerical modelling of the wave dynamics with the analytical predictions is performed. New phenomena of the resonances in the GC is found. The resonances occur for the waves polarized along the rotational axis having the smallest dumping due to the viscosity.

The American Gas Centrifuge Past, Present, and Future

Energy Technology Data Exchange (ETDEWEB)

Waters, Dean

2004-09-15

The art of gas centrifugation was born in 1935 at the University of Virginia when Dr. Jesse Beams demonstrated experimentally the separation of chlorine isotopes using an ultra-high speed centrifuge. Dr. Beam’s experiment initiated work that created a rich history of scientific and engineering accomplishment in the United States in the art of isotope separation and even large scale biological separation by centrifugation. The early history of the gas centrifuge development was captured in a lecture and documented by Dr. Jesse Beams in 1975. Much of Dr. Beams lecture material is used in this paper up to the year 1960. Following work by Dr. Gernot Zippe at the University of Virginia between 1958 and 1960, the US government embarked on a centrifuge development program that ultimately led to the start of construction of the Gas Centrifuge Enrichment Plant in Piketon Ohio in the late 1970’s. The government program was abandoned in 1985 after investing in the construction of two of six planned process buildings, a complete supply chain for process and centrifuge parts, and the successful manufacture and brief operation of an initial complement of production machines that would have met 15 percent of the planned capacity of the constructed process buildings. A declining market for enriched uranium, a glut of uranium enrichment capacity worldwide, and the promise of a new laser based separation process factored in the decision to stop the government program. By the late 1990’s it had become evident that gas centrifugation held the best promise to produce enriched uranium at low cost. In1999, the United States Enrichment Corporation undertook an initiative to revive the best of the American centrifuge technology that had been abandoned fourteen years earlier. This is an exciting story and one that when complete will enable the United States to maintain its domestic supply and to be highly competitive in the world market for this important energy commodity. (auth)

Protein removal from waste brines generated during ham salting through acidification and centrifugation.

Science.gov (United States)

Gutiérrez-Martínez, Maria del Rosario; Muñoz-Guerrero, Hernán; Alcaína-Miranda, Maria Isabel; Barat, José Manuel

2014-03-01

The salting step in food processes implies the production of large quantities of waste brines, having high organic load, high conductivity, and other pollutants with high oxygen demand. Direct disposal of the residual brine implies salinization of soil and eutrophication of water. Since most of the organic load of the waste brines comes from proteins leaked from the salted product, precipitation of dissolved proteins by acidification and removal by centrifugation is an operation to be used in waste brine cleaning. The aim of this study is optimizing the conditions for carrying out the separation of proteins from waste brines generated in the pork ham salting operation, by studying the influence of pH, centrifugal force, and centrifugation time. Models for determining the removal of proteins depending on the pH, centrifugal force, and time were obtained. The results showed a high efficacy of the proposed treatment for removing proteins, suggesting that this method could be used for waste brine protein removal. The best pH value to be used in an industrial process seems to be 3, while the obtained results indicate that almost 90% of the proteins from the brine can be removed by acidification followed by centrifugation. A further protein removal from the brine should have to be achieved using filtrating techniques, which efficiency could be highly improved as a consequence of the previous treatment through acidification and centrifugation. Waste brines from meat salting have high organic load and electrical conductivity. Proteins can be removed from the waste brine by acidification and centrifugation. The total protein removal can be up to 90% of the initial content of the waste brine. Protein removal is highly dependent on pH, centrifugation rate, and time. © 2014 Institute of Food Technologists®

Centrifugal extraction of highly enriched tin isotopes and increase of specific activity of the radionuclide 119mSn on the gas centrifuge cascade

International Nuclear Information System (INIS)

Suvorov, I.A.; Tcheltsov, A.N.; Sosnin, L.Yu.; Sazikin, A.A.; Rudnev, A.I.

2002-01-01

This work contains the results of research on centrifugal enrichment of 118 Sn isotope followed by irradiation and, finally, a second centrifugal enrichment to produce high specific activity 119m Sn. Non-steady-state separation methods were used for the effective extraction of the radionuclide 119m Sn from the irradiated target. As a result of this work, radiation sources based on 119m Sn were obtained with a specific activity of 500 mCi/g. This is 100 times greater than the specific activity obtained after irradiation in the reactor alone. In addition, the sources had an previously unattainable radio-purity ratio of 113 Sn/ 119m Sn of approximately 10 -6

AIChe equipment testing procedure centrifugal compressors : a guide to performance evaluation and site testing

CERN Document Server

AIChE

2013-01-01

With its engineer-tested procedures and thorough explanations, Centrifugal Compressors is an essential text for anyone engaged in implementing new technology in equipment design, identifying process problems, and optimizing equipment performance.  This condensed book presents a step by step approach to preparing for, planning, executing, and analyzing tests of centrifugal compressors, with an emphasis on methods that can be conducted on-site and with an acknowledgement of the strengths and limitations of these methods. The book opens with an extensive and detailed section offering definitions

$L_{0}$ Gradient Projection.

Science.gov (United States)

Ono, Shunsuke

2017-04-01

Minimizing L 0 gradient, the number of the non-zero gradients of an image, together with a quadratic data-fidelity to an input image has been recognized as a powerful edge-preserving filtering method. However, the L 0 gradient minimization has an inherent difficulty: a user-given parameter controlling the degree of flatness does not have a physical meaning since the parameter just balances the relative importance of the L 0 gradient term to the quadratic data-fidelity term. As a result, the setting of the parameter is a troublesome work in the L 0 gradient minimization. To circumvent the difficulty, we propose a new edge-preserving filtering method with a novel use of the L 0 gradient. Our method is formulated as the minimization of the quadratic data-fidelity subject to the hard constraint that the L 0 gradient is less than a user-given parameter α . This strategy is much more intuitive than the L 0 gradient minimization because the parameter α has a clear meaning: the L 0 gradient value of the output image itself, so that one can directly impose a desired degree of flatness by α . We also provide an efficient algorithm based on the so-called alternating direction method of multipliers for computing an approximate solution of the nonconvex problem, where we decompose it into two subproblems and derive closed-form solutions to them. The advantages of our method are demonstrated through extensive experiments.

New generalized conjugate gradient methods for the non-quadratic model in unconstrained optimization

International Nuclear Information System (INIS)

Al-Bayati, A.

2001-01-01

This paper present two new conjugate gradient algorithms which use the non-quadratic model in unconstrained optimization. The first is a new generalized self-scaling variable metric algorithm based on the sloboda generalized conjugate gradient method which is invariant to a nonlinear scaling of a stricity convex quadratic function; the second is an interleaving between the generalized sloboda method and the first algorithm; all these algorithm use exact line searches. Numerical comparisons over twenty test functions show that the interleaving algorithm is best overall and requires only about half the function evaluations of the Sloboda method: interleaving algorithms are likely to be preferred when the dimensionality of the problem is increased. (author). 29 refs., 1 tab

Single-layer centrifugation through colloid selects improved quality of epididymal cat sperm.

Science.gov (United States)

Chatdarong, K; Thuwanut, P; Morrell, J M

2010-06-01

The objectives were to determine the: 1) extent of epithelial and red blood cell contamination in epididymal cat sperm samples recovered by the cutting method; 2) efficacy of simple washing, single-layer centrifugation (SLC), and swim-up for selecting epididymal cat sperm; and 3) effects of freezing and thawing on cat sperm selected by various techniques. Ten unit samples were studied; each contained sperm from the cauda epididymides of four cats (total, approximately 200 x 10(6) sperm) and was equally allocated into four treatments: 1) simple washing, 2) single-layer centrifugation through colloid prior to cryopreservation (SLC-PC), 3) single-layer centrifugation through colloid after cryopreservation (SLC-AC), and 4) swim-up. Centrifugation (300 x g for 20 min) was done for all methods. The SLC-PC had a better recovery rate than the SLC-AC and swim-up methods (mean+/-SD of 16.4+/-8.7, 10.7+/-8.9, and 2.3+/-1.7%, respectively; Pblood cell contamination than simple washed samples (0.02+/-0.01, 0.02+/-0.04, 0.03+/-0.04, and 0.44+/-0.22 x 10(6) cells/mL, respectively; P0.05), SLC-PC yielded the highest percentage of sperm with normal midpieces and tails (P0.05). In conclusion, both SLC-PC and swim-up improved the quality of epididymal cat sperm, including better morphology, membrane and DNA integrity, and removal of cellular contamination. However, SLC had a better sperm recovery rate than swim-up. 2010 Elsevier Inc. All rights reserved.

Subjective stress factors in centrifuge training for military aircrews.

Science.gov (United States)

Lin, Pei-Chun; Wang, Jenhung; Li, Shih-Chin

2012-07-01

This study investigates stress-influence factors perceived by military aircrews undergoing centrifuge training, which lowers the incidence of G-induced loss of consciousness (G-LOC) for the crews of high-performance combat aircrafts. We used questionnaires to assess the subjective stress-influence factors of crews undergoing centrifuge training. Professionals in aviation physiology identified attributes measuring the perceived stress induced by centrifuge training, which were segmented into three constructs by factor analysis, theory lecture, centrifuge equipment, and physical fitness. Considerable interpenetration was discernible between these factors and military rank, age, length of service, flight hours accrued, and type of aircraft piloted. Identifying and quantifying the perceived stressors experienced in human-use centrifuge training enables aviators, astronauts, and air forces of the world to determine which constructs perceptibly increase or alleviate the perceived stress undergone by trainees when partaking in centrifuge training. Copyright © 2011 Elsevier Ltd and The Ergonomics Society. All rights reserved.

A family of conjugate gradient methods for large-scale nonlinear equations.

Science.gov (United States)

Feng, Dexiang; Sun, Min; Wang, Xueyong

2017-01-01

In this paper, we present a family of conjugate gradient projection methods for solving large-scale nonlinear equations. At each iteration, it needs low storage and the subproblem can be easily solved. Compared with the existing solution methods for solving the problem, its global convergence is established without the restriction of the Lipschitz continuity on the underlying mapping. Preliminary numerical results are reported to show the efficiency of the proposed method.

Fluorimetric methods for the measurement of intermediate metabolites (lactate, pyruvate, alanine, β-hydroxybutyrate, glycerol) using a COBAS FARA centrifugal analyser

OpenAIRE

Monti, L. D.; Sandoli, P. E.; Costa, S.; Phan, V. C.; Piatti, P. M.

1993-01-01

Intermediate products of the metabolism of glucose, fat and amino-acid are important in the evaluation of such metabolic disorders as diabetes mellitus, liver disease and metabolic acidosis. In the present study, methods for the measurement of intermediate metabolites (lactate, pyruvate, alanine, β-hydroxybutyrate and glycerol) have been adapted to a fast centrifugal analyzer: the COBAS FARA. Correlation coeffcients rangedfrom 0.90 to 0.99, compared to established manual spectrophotometric me...

Bifurcated equilibria in centrifugally confined plasma

International Nuclear Information System (INIS)

Shamim, I.; Teodorescu, C.; Guzdar, P. N.; Hassam, A. B.; Clary, R.; Ellis, R.; Lunsford, R.

2008-01-01

A bifurcation theory and associated computational model are developed to account for abrupt transitions observed recently on the Maryland Centrifugal eXperiment (MCX) [R. F. Ellis et al. Phys. Plasmas 8, 2057 (2001)], a supersonically rotating magnetized plasma that relies on centrifugal forces to prevent thermal expansion of plasma along the magnetic field. The observed transitions are from a well-confined, high-rotation state (HR-mode) to a lower-rotation, lesser-confined state (O-mode). A two-dimensional time-dependent magnetohydrodynamics code is used to simulate the dynamical equilibrium states of the MCX configuration. In addition to the expected viscous drag on the core plasma rotation, a momentum loss term is added that models the friction of plasma on the enhanced level of neutrals expected in the vicinity of the insulators at the throats of the magnetic mirror geometry. At small values of the external rotation drive, the plasma is not well-centrifugally confined and hence experiences the drag from near the insulators. Beyond a critical value of the external drive, the system makes an abrupt transition to a well-centrifugally confined state in which the plasma has pulled away from the end insulator plates; more effective centrifugal confinement lowers the plasma mass near the insulators allowing runaway increases in the rotation speed. The well-confined steady state is reached when the external drive is balanced by only the viscosity of the core plasma. A clear hysteresis phenomenon is shown.

Centrifugal acceleration in the magnetotail lobes