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Sample records for globular test protein

  1. Cold gelation of globular proteins

    NARCIS (Netherlands)

    Alting, A.C.

    2003-01-01

    Keywords : globular proteins, whey protein, ovalbumin, cold gelation, disulfide bonds, texture, gel hardnessProtein gelation in food products is important to obtain desirable sensory and textural properties. Cold gelation is a novel method to produce protein-based gels. It is a two step process in

  2. Nanofibers made of globular proteins.

    Science.gov (United States)

    Dror, Yael; Ziv, Tamar; Makarov, Vadim; Wolf, Hila; Admon, Arie; Zussman, Eyal

    2008-10-01

    Strong nanofibers composed entirely of a model globular protein, namely, bovine serum albumin (BSA), were produced by electrospinning directly from a BSA solution without the use of chemical cross-linkers. Control of the spinnability and the mechanical properties of the produced nanofibers was achieved by manipulating the protein conformation, protein aggregation, and intra/intermolecular disulfide bonds exchange. In this manner, a low-viscosity globular protein solution could be modified into a polymer-like spinnable solution and easily spun into fibers whose mechanical properties were as good as those of natural fibers made of fibrous protein. We demonstrate here that newly formed disulfide bonds (intra/intermolecular) have a dominant role in both the formation of the nanofibers and in providing them with superior mechanical properties. Our approach to engineer proteins into biocompatible fibrous structures may be used in a wide range of biomedical applications such as suturing, wound dressing, and wound closure.

  3. Statistical interior properties of globular proteins

    International Nuclear Information System (INIS)

    Zhou-Ting, Jiang; Tai-Quan, Wu; Lin-Xi, Zhang; Ting-Ting, Sun

    2009-01-01

    The character of forming long-range contacts affects the three-dimensional structure of globular proteins deeply. As the different ability to form long-range contacts between 20 types of amino acids and 4 categories of globular proteins, the statistical properties are thoroughly discussed in this paper. Two parameters N C and N D are defined to confine the valid residues in detail. The relationship between hydrophobicity scales and valid residue percentage of each amino acid is given in the present work and the linear functions are shown in our statistical results. It is concluded that the hydrophobicity scale defined by chemical derivatives of the amino acids and nonpolar phase of large unilamellar vesicle membranes is the most effective technique to characterise the hydrophobic behavior of amino acid residues. Meanwhile, residue percentage P i and sequential residue length L i of a certain protein i are calculated under different conditions. The statistical results show that the average value of P i as well as L i of all-α proteins has a minimum among these 4 classes of globular proteins, indicating that all-α proteins are hardly capable of forming long-range contacts one by one along their linear amino acid sequences. All-β proteins have a higher tendency to construct long-range contacts along their primary sequences related to the secondary configurations, i.e. parallel and anti-parallel configurations of β sheets. The investigation of the interior properties of globular proteins give us the connection between the three-dimensional structure and its primary sequence data or secondary configurations, and help us to understand the structure of protein and its folding process well. (cross-disciplinary physics and related areas of science and technology)

  4. Pyroelectricity in globular protein lysozyme films

    Science.gov (United States)

    Stapleton, A.; Noor, M. R.; Haq, E. U.; Silien, C.; Soulimane, T.; Tofail, S. A. M.

    2018-03-01

    Pyroelectricity is the ability of certain non-centrosymmetric materials to generate an electric charge in response to a change in temperature and finds use in a range of applications from burglar alarms to thermal imaging. Some biological materials also exhibit pyroelectricity but the examples of the effect are limited to fibrous proteins, polypeptides, and tissues and organs of animals and plants. Here, we report pyroelectricity in polycrystalline aggregate films of lysozyme, a globular protein.

  5. Computational and theoretical studies of globular proteins

    Science.gov (United States)

    Pagan, Daniel L.

    Protein crystallization is often achieved in experiment through a trial and error approach. To date, there exists a dearth of theoretical understanding of the initial conditions necessary to promote crystallization. While a better understanding of crystallization will help to create good crystals suitable for structure analysis, it will also allow us to prevent the onset of certain diseases. The core of this thesis is to model and, ultimately, understand the phase behavior of protein particles in solution. Toward this goal, we calculate the fluid-fluid coexistence curve in the vicinity of the metastable critical point of the modified Lennard-Jones potential, where it has been shown that nucleation is increased by many orders of magnitude. We use finite-size scaling techniques and grand canonical Monte Carlo simulation methods. This has allowed us to pinpoint the critical point and subcritical region with high accuracy in spite of the critical fluctuations that hinder sampling using other Monte Carlo techniques. We also attempt to model the phase behavior of the gamma-crystallins, mutations of which have been linked to genetic cataracts. The complete phase behavior of the square well potential at the ranges of attraction lambda = 1.15 and lambda = 1.25 is calculated and compared with that of the gammaII-crystallin. The role of solvent is also important in the crystallization process and affects the phase behavior of proteins in solution. We study a model that accounts for the contribution of the solvent free-energy to the free-energy of globular proteins. This model allows us to model phase behavior that includes solvent.

  6. Transiently disordered tails accelerate folding of globular proteins.

    Science.gov (United States)

    Mallik, Saurav; Ray, Tanaya; Kundu, Sudip

    2017-07-01

    Numerous biological proteins exhibit intrinsic disorder at their termini, which are associated with multifarious functional roles. Here, we show the surprising result that an increased percentage of terminal short transiently disordered regions with enhanced flexibility (TstDREF) is associated with accelerated folding rates of globular proteins. Evolutionary conservation of predicted disorder at TstDREFs and drastic alteration of folding rates upon point-mutations suggest critical regulatory role(s) of TstDREFs in shaping the folding kinetics. TstDREFs are associated with long-range intramolecular interactions and the percentage of native secondary structural elements physically contacted by TstDREFs exhibit another surprising positive correlation with folding kinetics. These results allow us to infer probable molecular mechanisms behind the TstDREF-mediated regulation of folding kinetics that challenge protein biochemists to assess by direct experimental testing. © 2017 Federation of European Biochemical Societies.

  7. Microwave-enhanced folding and denaturation of globular proteins

    DEFF Research Database (Denmark)

    Bohr, Henrik; Bohr, Jakob

    2000-01-01

    It is shown that microwave irradiation can affect the kinetics of the folding process of some globular proteins, especially beta-lactoglobulin. At low temperature the folding from the cold denatured phase of the protein is enhanced, while at a higher temperature the denaturation of the protein from...... its folded state is enhanced. In the latter case, a negative temperature gradient is needed for the denaturation process, suggesting that the effects of the microwaves are nonthermal. This supports the notion that coherent topological excitations can exist in proteins. The application of microwaves...

  8. [Study of beta-turns in globular proteins].

    Science.gov (United States)

    Amirova, S R; Milchevskiĭ, Iu V; Filatov, I V; Esipova, N G; Tumanian, V G

    2005-01-01

    The formation of beta-turns in globular proteins has been studied by the method of molecular mechanics. Statistical method of discriminant analysis was applied to calculate energy components and sequences of oligopeptide segments, and after this prediction of I type beta-turns has been drawn. The accuracy of true positive prediction is 65%. Components of conformational energy considerably affecting beta-turn formation were delineated. There are torsional energy, energy of hydrogen bonds, and van der Waals energy.

  9. Dynamic prestress in a globular protein.

    Directory of Open Access Journals (Sweden)

    Scott A Edwards

    Full Text Available A protein at equilibrium is commonly thought of as a fully relaxed structure, with the intra-molecular interactions showing fluctuations around their energy minimum. In contrast, here we find direct evidence for a protein as a molecular tensegrity structure, comprising a balance of tensed and compressed interactions, a concept that has been put forward for macroscopic structures. We quantified the distribution of inter-residue prestress in ubiquitin and immunoglobulin from all-atom molecular dynamics simulations. The network of highly fluctuating yet significant inter-residue forces in proteins is a consequence of the intrinsic frustration of a protein when sampling its rugged energy landscape. In beta sheets, this balance of forces is found to compress the intra-strand hydrogen bonds. We estimate that the observed magnitude of this pre-compression is enough to induce significant changes in the hydrogen bond lifetimes; thus, prestress, which can be as high as a few 100 pN, can be considered a key factor in determining the unfolding kinetics and pathway of proteins under force. Strong pre-tension in certain salt bridges on the other hand is connected to the thermodynamic stability of ubiquitin. Effective force profiles between some side-chains reveal the signature of multiple, distinct conformational states, and such static disorder could be one factor explaining the growing body of experiments revealing non-exponential unfolding kinetics of proteins. The design of prestress distributions in engineering proteins promises to be a new tool for tailoring the mechanical properties of made-to-order nanomaterials.

  10. Globular conformation of some ribosomal proteins in solution

    International Nuclear Information System (INIS)

    Serdyuk, I.N.; Spirin, A.S.

    1978-01-01

    The possibility that such RNA-binding proteins of the 30 S subparticle as S4, S7, S8 and S16 exist in the form of compact globules in solution has been explored experimentally. These proteins have been studied in D 2 O solution by neutron scattering to measure their radii of gyration. This type of radiation using D 2 O as a solvent provides the maximum 'contrast', that is the maximum difference between the scattering of the protein and the solvent. It allowed measurements to be made using protein at <= 1.5 mg/ml. The radii of gyration for the ribosomal proteins S4, S7, S8 and S16 were found to be relatively small corresponding to the radii of gyration of compact globular proteins of the same molecular weights. (Auth.)

  11. Interactions between globular proteins and F-actin in isotonic saline solution.

    Science.gov (United States)

    Lakatos, S; Minton, A P

    1991-10-05

    Solutions of each of three different globular proteins (cytochrome c, chromophorically labeled serum albumin, and chromophorically labeled aldolase), mixed with another unlabeled globular protein or with fibrous actin, were prepared in pH 8.0 Tris-HCl buffer containing 0.15 M NaCl. Each solution was centrifuged at low speed, at 5 degrees C, until unassociated globular protein in solution achieved sedimentation equilibrium. Individual absorbance gradients of both macrosolutes in the mixtures subsequent to centrifugation were obtained via optical scans of the centrifuge tubes at two wavelengths. The gradients of each macrosolute in mixtures of two globular proteins revealed no association of globular proteins under the conditions of these experiments, but perturbation of the gradients of serum albumin, aldolase, and cytochrome c in the presence of F-actin indicated association of all three globular proteins with F-actin. Perturbation of actin gradients in the presence of serum albumin and aldolase suggested partial depolymerization of the F-actin by the globular protein. Analysis of the data with a simple phenomenological model relating free globular protein, bound globular protein, and total actin concentration provided estimates of the respective equilibrium constants for association of serum albumin and aldolase with F-actin, under the conditions of these experiments, of the order of 0.1 microM-1.

  12. Dynamics of proteins at low temperatures: fibrous vs. globular

    Science.gov (United States)

    Foucat, L.; Renou, J.-P.; Tengroth, C.; Janssen, S.; Middendorf, H. D.

    We have measured quasielastic neutron scattering from H2O-hydrated collagen and haemoglobin at Tτ>10 ps. Relative to haemoglobin, the 200-K dynamic transition is shifted upward by 20-25 K in collagen, and the T-dependence of m.-sq. displacements derived from Sqe(Q;T) suggests that in triple-helical systems there are three rather than two regimes: one up to around 120K (probably purely harmonic), an intermediate quasiharmonic region with a linear dependence up to 240K, followed by a steeper nonlinear rise similar to that in globular proteins.

  13. The direct piezoelectric effect in the globular protein lysozyme

    Science.gov (United States)

    Stapleton, A.; Noor, M. R.; Sweeney, J.; Casey, V.; Kholkin, A. L.; Silien, C.; Gandhi, A. A.; Soulimane, T.; Tofail, S. A. M.

    2017-10-01

    Here, we present experimental evidence of the direct piezoelectric effect in the globular protein, lysozyme. Piezoelectric materials are employed in many actuating and sensing applications because they can convert mechanical energy into electrical energy and vice versa. Although originally studied in inorganic materials, several biological materials including amino acids and bone, also exhibit piezoelectricity. The exact mechanisms supporting biological piezoelectricity are not known, nor is it known whether biological piezoelectricity conforms strictly to the criteria of classical piezoelectricity. The observation of piezoelectricity in protein crystals presented here links biological piezoelectricity with the classical theory of piezoelectricity. We quantify the direct piezoelectric effect in monoclinic and tetragonal aggregate films of lysozyme using conventional techniques based on the Berlincourt Method. The largest piezoelectric effect measured in a crystalline aggregate film of lysozyme was approximately 6.5 pC N-1. These findings raise fundamental questions as to the possible physiological significance of piezoelectricity in lysozyme and the potential for technical applications.

  14. Strong Keratin-like Nanofibers Made of Globular Protein

    Science.gov (United States)

    Dror, Yael; Makarov, Vadim; Admon, Arie; Zussman, Eyal

    2008-03-01

    Protein fibers as elementary structural and functional elements in nature inspire the engineering of protein-based products for versatile bio-medical applications. We have recently used the electrospinning process to fabricate strong sub-micron fibers made solely of serum albumin (SA). This raises the challenges of turning a globular non-viscous protein solution into a polymer--like spinnable solution and producing keratin-like fibers enriched in inter S-S bridges. A stable spinning process was achieved by using SA solution in a rich trifluoroethanol-water mixture with β-mercaptoethanol. The breakage of the intra disulfide bridges, as identified by mass spectrometry, together with the denaturing alcohol, enabled a pronounced expansion of the protein. This in turn, affects the rheological properties of the solution. X-ray diffraction pattern of the fibers revealed equatorial orientation, indicating the alignment of structures along the fiber axis. The mechanical properties reached remarkable average values (Young's modulus of 1.6GPa, and max stress of 36MPa) as compared to other fibrous protein nanofibers. These significant results are attributed to both the alignment and inter disulfide bonds (cross linking) that were formed by spontaneous post-spinning oxidation.

  15. The nature of folded states of globular proteins.

    Science.gov (United States)

    Honeycutt, J D; Thirumalai, D

    1992-06-01

    We suggest, using dynamical simulations of a simple heteropolymer modelling the alpha-carbon sequence in a protein, that generically the folded states of globular proteins correspond to statistically well-defined metastable states. This hypothesis, called the metastability hypothesis, states that there are several free energy minima separated by barriers of various heights such that the folded conformations of a polypeptide chain in each of the minima have similar structural characteristics but have different energies from one another. The calculated structural characteristics, such as bond angle and dihedral angle distribution functions, are assumed to arise from only those configurations belonging to a given minimum. The validity of this hypothesis is illustrated by simulations of a continuum model of a heteropolymer whose low temperature state is a well-defined beta-barrel structure. The simulations were done using a molecular dynamics algorithm (referred to as the "noisy" molecular dynamics method) containing both friction and noise terms. It is shown that for this model there are several distinct metastable minima in which the structural features are similar. Several new methods of analyzing fluctuations in structures belonging to two distinct minima are introduced. The most notable one is a dynamic measure of compactness that can in principle provide the time required for maximal compactness to be achieved. The analysis shows that for a given metastable state in which the protein has a well-defined folded structure the transition to a state of higher compactness occurs very slowly, lending credence to the notion that the system encounters a late barrier in the process of folding to the most compact structure. The examination of the fluctuations in the structures near the unfolding----folding transition temperature indicates that the transition state for the unfolding to folding process occurs closer to the folded state.

  16. Microstructure and rheology of globular protein gels in the presence of gelatin

    NARCIS (Netherlands)

    Ersch, C.; Meinders, M/B.J.; Bouwman, W.G.; Nieuwland, M.; Linden, E. van der; Venema, P.; Martin, A.H.

    2016-01-01

    The microstructure and rheological response of globular protein gels (whey protein isolate (WPI) and soy protein isolate (SPI)) in the presence of gelatin (type A, type B and hydrolyzed type A) was investigated. Microstructural information was obtained using a combination of confocal laser scanning

  17. Hydrophobicity diversity in globular and nonglobular proteins measured with the Gini index.

    Science.gov (United States)

    Carugo, Oliviero

    2017-12-01

    Amino acids and their properties are variably distributed in proteins and different compositions determine all protein features, ranging from solubility to stability and functionality. Gini index, a tool to estimate distribution uniformity, is widely used in macroeconomics and has numerous statistical applications. Here, Gini index is used to analyze the distribution of hydrophobicity in proteins and to compare hydrophobicity distribution in globular and intrinsically disordered proteins. Based on the analysis of carefully selected high-quality data sets of proteins extracted from the Protein Data Bank (http://www.rcsb.org) and from the DisProt database (http://www.disprot.org/), it is observed that hydrophobicity is distributed in a more diverse way in intrinsically disordered proteins than in folded and soluble globular proteins. This correlates with the observation that the amino acid composition deviates from the uniformity (estimate with the Shannon and the Gini-Simpson indices) more in intrinsically disordered proteins than in globular and soluble proteins. Although statistical tools tike the Gini index have received little attention in molecular biology, these results show that they allow one to estimate sequence diversity and that they are useful to delineate trends that can hardly be described, otherwise, in simple and concise ways. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  18. Modular organization of proteins containing C1q-like globular domain.

    Science.gov (United States)

    Kishore, U; Reid, K B

    1999-05-01

    The first step in the activation of the classical pathway of complement cascade by immune complexes involves the binding of the six globular heads of C1q to the Fc regions of immunoglobulin G (IgG) or immunoglobulin M (IgM). The globular heads of C1q are located C-terminal to the six triple-helical stalks present in the molecule, each head is considered to be composed of the C-terminal halves (3 x 135 residues) of one A-, one B- and one C-chain. It is not known if the C-terminal globular regions, present in each of the three types of chains, are independently folded modules (with each chain having distinct binding properties towards immunoglobulins) or whether the different binding functions of C1q are dependent upon a globular structure which relies on contributions from all three chains. Recent reports of recombinant production and characterisation of soluble globular head regions of all the three chains indicate that the globular regions of C1q may adopt a modular organization, i.e., each globular head of C1q may be composed of three, structurally and functionally, independent domains, thus retaining multivalency in the form of a heterotrimer. Modules of the same type as the C1q C-terminal module are also found in a variety of noncomplement proteins that include the C-terminal regions of the human type VIII and type X collagens, precerebellin, the chipmunk hibernation proteins, the human endothelial cell protein, multimerin, the serum protein, Acrp-30 which is secreted from mouse adipocytes, and the sunfish inner-ear specific structural protein. The C1q molecule is the only one of these proteins for which, to date, a function has been ascribed to the module. The existence of a shared structural region between C1q and certain collagens may suggest an evolutionarily common ancestral precursor. Various structural and biochemical data suggest that these modules may be responsible for multimerisation through patches of aromatic residues within them.

  19. A KINETIC MODEL FOR MONO-LAYER GLOBULAR PROTEIN ADSORPTION ON SOLID/LIQUID INTERFACES

    Directory of Open Access Journals (Sweden)

    Kamal I. M. Al-Malah

    2012-12-01

    Full Text Available A kinetic model was derived for globular protein adsorption. The model takes into account the three possible scenarios of a protein molecule in solution, being exposed to an interface: adsorption step from the solution to the interface; the possible desorption back into the solution; and the surface-induced unfolding or spreading of the protein unto the substrate surface. A globular protein molecule is visualized as a sphere with radius D. In addition to the general case of protein adsorption, which portrays either the surface coverage (Theta or surface concentration (� as a function of the adsorption time, special cases, like equilibrium condition, lowsurface coverage, irreversible, and Langmuirian were also presented and treated in light of the derived model. The general model was simplified for each of the subset cases. The irreversibility versus reversibility of protein adsorption was discussed. The substrate surface energetics or effects are accounted for via the proposition of the percent relative change in D/V ratio for the adsorbing protein, called (D/VPRC parameter. (D/VPRC is calculated with respect to the monolayer surface concentration of protein, where the latter is given by D/Vratio. This can be used as a landmark to protein adsorption isotherms or even kinetics. This is visualized as an indicator for solid substrate effects on the adsorbing proteins. (D/VPRC can be zero (fresh monolayer, negative (aged monolayer, or positive (multi-layer. The reference surface concentration is reported for some selected proteins.

  20. The small angle x-ray scattering of globular proteins in solution during heat denaturation

    Science.gov (United States)

    Banuelos, Jose; Urquidi, Jacob

    2008-10-01

    The ability of proteins to change their conformation in response to changes in their environment has consequences in biological processes like metabolism, chemical regulation in cells, and is believed to play a role in the onset of several neurodegenerative diseases. Factors such as a change in temperature, pressure, and the introduction of ions into the aqueous environment of a protein can give rise to the folding/unfolding of a protein. As a protein unfolds, the ratio of nonpolar to polar groups exposed to water changes, affecting a protein's thermodynamic properties. Using small angle x-ray scattering (SAXS), we are currently studying the intermediate protein conformations that arise during the folding/unfolding process as a function of temperature for five globular proteins. Trends in the observed intermediate structures of these globular proteins, along with correlations with data on protein thermodynamics may help elucidate shared characteristics between all proteins in the folding/unfolding process. Experimental design considerations will be discussed and preliminary results for some of these systems will be presented.

  1. Co-evolutionary constraints of globular proteins correlate with their folding rates.

    Science.gov (United States)

    Mallik, Saurav; Kundu, Sudip

    2015-08-04

    Folding rates (lnkf) of globular proteins correlate with their biophysical properties, but relationship between lnkf and patterns of sequence evolution remains elusive. We introduce 'relative co-evolution order' (rCEO) as length-normalized average primary chain separation of co-evolving pairs (CEPs), which negatively correlates with lnkf. In addition to pairs in native 3D contact, indirectly connected and structurally remote CEPs probably also play critical roles in protein folding. Correlation between rCEO and lnkf is stronger in multi-state proteins than two-state proteins, contrasting the case of contact order (co), where stronger correlation is found in two-state proteins. Finally, rCEO, co and lnkf are fitted into a 3D linear correlation. Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  2. Infrared and laser-Raman spectroscopic studies of thermally-induced globular protein gels.

    Science.gov (United States)

    Clark, A H; Saunderson, D H; Suggett, A

    1981-03-01

    Infrared and laser-Raman spectroscopy have been used to follow secondary structure changes during the heat-set gelation of a number of aqueous (D2O) globular protein solutions. Measurements of the infrared Amide I' absorption band around 1650 cm-1, for BSA gels of varying clarity and texture, have shown that the very considerable variations in network structure underlying these materials are not reflected in obvious differences in secondary structure. In all cases aggregation is accompanied by development of beta-sheet of a kind common in fibrous protein systems, but for BSA at least this does not appear to vary significantly in amount from one gel type to another. Infrared studies of gels formed from other protein systems have confirmed this tendency for beta-sheet to develop during aggregation, and the tendency is further substantiated by laser-Raman evidence which provides the extra information that in most of the examples studied alpha-helix content simultaneously falls. From these, and other observations, some generalisations are made about the thermally-induced sol-to-gel transformations of globular proteins.

  3. SANS study of understanding mechanism of cold gelation of globular proteins

    International Nuclear Information System (INIS)

    Chinchalikar, A. J.; Kumar, Sugam; Aswal, V. K.; Wagh, A. G.; Kohlbrecher, J.

    2014-01-01

    Small-angle neutron scattering (SANS) has been used to probe the evolution of interaction and the resultant structures in the cold gelation of globular proteins. The cold gelation involves two steps consisting of irreversible protein deformation by heating followed by some means (e.g. increasing ionic strength) to bring them together at room temperature. We have examined the role of different salts in cold gelation of preheated aqueous Bovine Serum Albumin (BSA) protein solutions. The interactions have been modeled by two Yukawa potential combining short-range attraction and long-range repulsion. We show that in step 1 (preheated temperature effect) the deformation of protein increases the magnitude of attractive interaction but not sufficient to induce gel. The attractive interaction is further enhanced in step 2 (salt effect) to result in gel formation. The salt effect is found to be strongly depending on the valency of the counterions. The gel structure has been characterized by the mass fractals

  4. Globular and disordered-the non-identical twins in protein-protein interactions

    DEFF Research Database (Denmark)

    Teilum, Kaare; Olsen, Johan Gotthardt; Kragelund, Birthe Brandt

    2015-01-01

    as a strong determinant for their function. This has fostered the notion that IDP's bind with low affinity but high specificity. Here we have analyzed available detailed thermodynamic data for protein-protein interactions to put to the test if the thermodynamic profiles of IDP interactions differ from those...... of other protein-protein interactions. We find that ordered proteins and the disordered ones act as non-identical twins operating by similar principles but where the disordered proteins complexes are on average less stable by 2.5 kcal mol(-1)....

  5. Globular and disordered – the non-identical twins in protein-protein interactions

    Directory of Open Access Journals (Sweden)

    Kaare eTeilum

    2015-07-01

    Full Text Available In biology proteins from different structural classes interact across and within classes in ways that are optimized to achieve balanced functional outputs. The interactions between intrinsically disordered proteins (IDPs and other proteins rely on changes in flexibility and this is seen as a strong determinant for their function. This has fostered the notion that IDP’s bind with low affinity but high specificity. Here we have analyzed available detailed thermodynamic data for protein-protein interactions to put to the test if the thermodynamic profiles of IDP interactions differ from those of other protein-protein interactions. We find that ordered proteins and the disordered ones act as non identical twins operating by similar principles but where the disordered proteins complexes are on average less stable by 2.5 kcal mol-1.

  6. Omnipresence of the polyproline II helix in fibrous and globular proteins.

    Science.gov (United States)

    Esipova, Natalia G; Tumanyan, Vladimir G

    2017-02-01

    Left-handed helical conformation of a polypeptide chain (PPII) is the third type of the protein backbone structure. This conformation universally exists in fibrous, globular proteins, and biologically active peptides. It has unique physical and chemical properties determining a wide range of biological functions, from the protein folding to the tissue differentiation. New examples of the structure have been appearing in spite of difficulties in their detection and investigation. The annotation and prediction of the PPII was also a challenging task. Recently, many PPII motifs with new and/or unexpected functions are being accumulated in databases. In this review we describe the major structural and dynamic forms of PPII, the diversity of its functions, and the role in different biological processes. Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. The recombinant globular head domain of the measles virus hemagglutinin protein as a subunit vaccine against measles.

    Science.gov (United States)

    Lobanova, Liubov M; Eng, Nelson F; Satkunarajah, Malathy; Mutwiri, George K; Rini, James M; Zakhartchouk, Alexander N

    2012-04-26

    Despite the availability of live attenuated measles virus (MV) vaccines, a large number of measles-associated deaths occur among infants in developing countries. The development of a measles subunit vaccine may circumvent the limitations associated with the current live attenuated vaccines and eventually contribute to global measles eradication. Therefore, the goal of this study was to test the feasibility of producing the recombinant globular head domain of the MV hemagglutinin (H) protein by stably transfected human cells and to examine the ability of this recombinant protein to elicit MV-specific immune responses. The recombinant protein was purified from the culture supernatant of stably transfected HEK293T cells secreting a tagged version of the protein. Two subcutaneous immunizations with the purified recombinant protein alone resulted in the production of MV-specific serum IgG and neutralizing antibodies in mice. Formulation of the protein with adjuvants (polyphosphazene or alum) further enhanced the humoral immune response and in addition resulted in the induction of cell-mediated immunity as measured by the production of MV H-specific interferon gamma (IFN-γ) and interleukin 5 (IL-5) by in vitro re-stimulated splenocytes. Furthermore, the inclusion of polyphosphazene into the vaccine formulation induced a mixed Th1/Th2-type immune response. In addition, the purified recombinant protein retained its immunogenicity even after storage at 37°C for 2 weeks. Copyright © 2012 Elsevier Ltd. All rights reserved.

  8. Effect of a low-frequency magnetic field on the structure of globular blood proteins

    Science.gov (United States)

    Zalesskaya, G. A.; Ulashchik, V. S.; Mit'kovskaya, N. P.; Laskina, O. V.; Kuchinskii, A. V.

    2007-09-01

    We used IR Fourier absorption spectra of blood to study changes in the structure of globular blood proteins with extracorporeal autohemomagnetotherapy, used to treat ischemic heart disease. We compare the spectra of blood before and after magnetotherapy in the regions: amide I (1655 cm-1), amide II (1545 cm-1), amide III (1230-1350 cm-1), amide IV and amide V (400-700 cm-1). We have shown that pronounced changes in the spectra in the indicated regions on direct exposure of blood in vivo to a low-frequency pulsed magnetic field are connected with conformational changes in the secondary structure of globular blood proteins, which are apparent in the increase in the contribution of the α-helix conformation. We discuss the magnetotherapy-initiated appearance of new IR absorption bands at 1018 and 1038 cm-1 and an increase in the intensity of a number of other bands located in the 1000-1200 cm-1 region, which suggests a change in the concentration of some blood components.

  9. CONSTRAINTS ON HELIUM ENHANCEMENT IN THE GLOBULAR CLUSTER M3 (NGC 5272): THE HORIZONTAL BRANCH TEST

    International Nuclear Information System (INIS)

    Catelan, M.; Valcarce, A. A. R.; Cortes, C.; Grundahl, F.; Sweigart, A. V.

    2009-01-01

    It has recently been suggested that the presence of multiple populations showing various amounts of helium enhancement is the rule, rather than the exception, among globular star clusters. An important prediction of this helium enhancement scenario is that the helium-enhanced blue horizontal branch (HB) stars should be brighter than the red HB stars which are not helium enhanced. In this Letter, we test this prediction in the case of the Galactic globular cluster M3 (NGC 5272), for which the helium-enhancement scenario predicts helium enhancements of ∼>0.02 in virtually all blue HB stars. Using high-precision Stroemgren photometry and spectroscopic gravities for blue HB stars, we find that any helium enhancement among most of the cluster's blue HB stars is very likely less than 0.01, thus ruling out the much higher helium enhancements that have been proposed in the literature.

  10. Small-angle X-ray scattering studies of thermally-induced globular protein gels

    International Nuclear Information System (INIS)

    Clark, A.H.; Tuffnell, C.D.

    1980-01-01

    Small-angle X-ray scattering has been applied to gels formed by heating globular proteins, in aqueous solution, above their unfolding temperatures. A number of BSA gels, previously characterised by electron microscopy, have been studied, and by setting up theoretical models for the scattering process, the X-ray data have been shown to be consistent with the microscope conclusions regarding network structure. It is concluded that the networks form by a linearly-directed aggregation of unfolded, disc-like, protein molecules, three-dimensional geometry being achieved by occasional branching, and/or cross-linking. Long-range inhomogeneities in network structure, easily observed by electron microscopy, and correlated with variations in pH or ionic strength, have an effect on X-ray scattering, and hence the X-ray method is sensitive not only to different network strand thicknesses, but to different degrees of uniformity as well. (author)

  11. Testing modified gravity with globular clusters: the case of NGC 2419

    Science.gov (United States)

    Llinares, Claudio

    2018-05-01

    The dynamics of globular clusters has been studied in great detail in the context of general relativity as well as with modifications of gravity that strongly depart from the standard paradigm such as Modified Newtonian Dynamics. However, at present there are no studies that aim to test the impact that less extreme modifications of gravity (e.g. models constructed as alternatives to dark energy) have on the behaviour of globular clusters. This Letter presents fits to the velocity dispersion profile of the cluster NGC 2419 under the symmetron-modified gravity model. The data show an increase in the velocity dispersion towards the centre of the cluster which could be difficult to explain within general relativity. By finding the best-fitting solution associated with the symmetron model, we show that this tension does not exist in modified gravity. However, the best-fitting parameters give a model that is inconsistent with the dynamics of the Solar system. Exploration of different screening mechanisms should give us the chance to understand if it is possible to maintain the appealing properties of the symmetron model when it comes to globular clusters and at the same time recover the Solar system dynamics properly.

  12. Salt-bridge networks within globular and disordered proteins: characterizing trends for designable interactions.

    Science.gov (United States)

    Basu, Sankar; Mukharjee, Debasish

    2017-07-01

    There has been considerable debate about the contribution of salt bridges to the stabilization of protein folds, in spite of their participation in crucial protein functions. Salt bridges appear to contribute to the activity-stability trade-off within proteins by bringing high-entropy charged amino acids into close contacts during the course of their functions. The current study analyzes the modes of association of salt bridges (in terms of networks) within globular proteins and at protein-protein interfaces. While the most common and trivial type of salt bridge is the isolated salt bridge, bifurcated salt bridge appears to be a distinct salt-bridge motif having a special topology and geometry. Bifurcated salt bridges are found ubiquitously in proteins and interprotein complexes. Interesting and attractive examples presenting different modes of interaction are highlighted. Bifurcated salt bridges appear to function as molecular clips that are used to stitch together large surface contours at interacting protein interfaces. The present work also emphasizes the key role of salt-bridge-mediated interactions in the partial folding of proteins containing long stretches of disordered regions. Salt-bridge-mediated interactions seem to be pivotal to the promotion of "disorder-to-order" transitions in small disordered protein fragments and their stabilization upon binding. The results obtained in this work should help to guide efforts to elucidate the modus operandi of these partially disordered proteins, and to conceptualize how these proteins manage to maintain the required amount of disorder even in their bound forms. This work could also potentially facilitate explorations of geometrically specific designable salt bridges through the characterization of composite salt-bridge networks. Graphical abstract ᅟ.

  13. AFM-based identification of the dynamic properties of globular proteins: simulation study

    International Nuclear Information System (INIS)

    Kim, Deok Ho; Park, Jung Yul; Kim, Moon K.; Hong, Keum Shik

    2008-01-01

    Nowadays a mathematical model-based computational approach is getting more attention as an effective tool for understanding the mechanical behaviors of biological systems. To find the mechanical properties of the proteins required to build such a model, this paper investigates a real-time identification method based on an AFM nanomanipulation system. First, an AFM-based bio-characterization system is introduced. Second, a second-order time-varying linear model representing the interaction between an AFM cantilever and globular proteins in a solvent is presented. Finally, we address a real-time estimation method in which the results of AFM experiments are designed to be inputs of the state estimator proposed here. Our attention is restricted to a theoretical feasibility analysis of the proposed methodology. We simply set the mechanical properties of the particular protein such as mass, stiffness, and damping coefficient in the system model prior to running the simulation. Simulation results show very good agreement with the preset properties. We anticipate that the realization of the AFM-based bio-characterization system will also provide an experimental validation of the proposed identification procedure in the future. This methodology can be used to determine a model of protein motion for the purpose of computer simulation and for a real-time modification of protein deformation

  14. Identifying the adaptive mechanism in globular proteins: Fluctuations in densely packed regions manipulate flexible parts

    Science.gov (United States)

    Yilmaz, Lutfu Safak; Atilgan, Ali Rana

    2000-09-01

    A low-resolution structural model based on the packing geometry of α-carbons is utilized to establish a connection between the flexible and rigid parts of a folded protein. The former commonly recognizes a complementing molecule for making a complex, while the latter manipulates the necessary conformational change for binding. We attempt analytically to distinguish this control architecture that intrinsically exists in globular proteins. First with two-dimensional simple models, then for a native protein, bovine pancreatic trypsin inhibitor, we explicitly demonstrate that inserting fluctuations in tertiary contacts supported by the stable core, one can regulate the displacement of residues on loop regions. The positional fluctuations of the flexible regions are annihilated by the rest of the protein in conformity with the Le Chatelier-Braun principle. The results indicate that the distortion of the principal nonbonded contacts between highly packed residues is accompanied by that of the slavery fluctuations that are widely distributed over the native structure. These positional arrangements do not appear in a reciprocal relation between a perturbation and the associated response; the effect of a movement of residue i on residue j is not equal to that of the same movement of residue j on residue i.

  15. Description of atomic burials in compact globular proteins by Fermi-Dirac probability distributions.

    Science.gov (United States)

    Gomes, Antonio L C; de Rezende, Júlia R; Pereira de Araújo, Antônio F; Shakhnovich, Eugene I

    2007-02-01

    We perform a statistical analysis of atomic distributions as a function of the distance R from the molecular geometrical center in a nonredundant set of compact globular proteins. The number of atoms increases quadratically for small R, indicating a constant average density inside the core, reaches a maximum at a size-dependent distance R(max), and falls rapidly for larger R. The empirical curves turn out to be consistent with the volume increase of spherical concentric solid shells and a Fermi-Dirac distribution in which the distance R plays the role of an effective atomic energy epsilon(R) = R. The effective chemical potential mu governing the distribution increases with the number of residues, reflecting the size of the protein globule, while the temperature parameter beta decreases. Interestingly, betamu is not as strongly dependent on protein size and appears to be tuned to maintain approximately half of the atoms in the high density interior and the other half in the exterior region of rapidly decreasing density. A normalized size-independent distribution was obtained for the atomic probability as a function of the reduced distance, r = R/R(g), where R(g) is the radius of gyration. The global normalized Fermi distribution, F(r), can be reasonably decomposed in Fermi-like subdistributions for different atomic types tau, F(tau)(r), with Sigma(tau)F(tau)(r) = F(r), which depend on two additional parameters mu(tau) and h(tau). The chemical potential mu(tau) affects a scaling prefactor and depends on the overall frequency of the corresponding atomic type, while the maximum position of the subdistribution is determined by h(tau), which appears in a type-dependent atomic effective energy, epsilon(tau)(r) = h(tau)r, and is strongly correlated to available hydrophobicity scales. Better adjustments are obtained when the effective energy is not assumed to be necessarily linear, or epsilon(tau)*(r) = h(tau)*r(alpha,), in which case a correlation with hydrophobicity

  16. Chromosomal rearrangements and protein globularity changes in Mycobacterium tuberculosis isolates from cerebrospinal fluid

    Directory of Open Access Journals (Sweden)

    Seow Hoon Saw

    2016-09-01

    Full Text Available Background Meningitis is a major cause of mortality in tuberculosis (TB. It is not clear what factors promote central nervous system invasion and pathology but it has been reported that certain strains of Mycobacterium tuberculosis (Mtb might have genetic traits associated with neurotropism. Methods In this study, we generated whole genome sequences of eight clinical strains of Mtb that were isolated from the cerebrospinal fluid (CSF of patients presenting with tuberculous meningitis (TBM in Malaysia, and compared them to the genomes of H37Rv and other respiratory Mtb genomes either downloaded from public databases or extracted from local sputum isolates. We aimed to find genomic features that might be distinctly different between CSF-derived and respiratory Mtb. Results Genome-wide comparisons revealed rearrangements (translocations, inversions, insertions and deletions and non-synonymous SNPs in our CSF-derived strains that were not observed in the respiratory Mtb genomes used for comparison. These rearranged segments were rich in genes for PE (proline-glutamate/PPE (proline-proline-glutamate, transcriptional and membrane proteins. Similarly, most of the ns SNPs common in CSF strains were noted in genes encoding PE/PPE proteins. Protein globularity differences were observed among mycobacteria from CSF and respiratory sources and in proteins previously reported to be associated with TB meningitis. Transcription factors and other transcription regulators featured prominently in these proteins. Homologs of proteins associated with Streptococcus pneumoniae meningitis and Neisseria meningitidis virulence were identified in neuropathogenic as well as respiratory mycobacterial spp. examined in this study. Discussion The occurrence of in silico genetic differences in CSF-derived but not respiratory Mtb suggests their possible involvement in the pathogenesis of TBM. However, overall findings in this comparative analysis support the postulation that TB

  17. Constraints on helium enhancement in the globular cluster M4 (NGC 6121): The horizontal branch test

    Energy Technology Data Exchange (ETDEWEB)

    Valcarce, A. A. R.; De Medeiros, J. R. [Universidade Federal do Rio Grande do Norte, Departamento de Física, 59072-970 Natal, RN (Brazil); Catelan, M. [Pontificia Universidad Católica de Chile, Centro de Astroingeniería, Av. Vicuña Mackena 4860, 782-0436 Macul, Santiago (Chile); Alonso-García, J. [Pontificia Universidad Católica de Chile, Instituto de Astrofísica, Facultad de Física, Av. Vicuña Mackena 4860, 782-0436 Macul, Santiago (Chile); Cortés, C. [Universidad Metropolitana de Ciencias de la Educación, Facultad de Ciencias Básicas, Departamento de Física, Av. José Pedro Alessandri 774, Santiago (Chile)

    2014-02-20

    Recent pieces of evidence have revealed that most, and possibly all, globular star clusters are composed of groups of stars that formed in multiple episodes with different chemical compositions. In this sense, it has also been argued that variations in the initial helium abundance (Y) from one population to the next are also the rule, rather than the exception. In the case of the metal-intermediate globular cluster M4 (NGC 6121), recent high-resolution spectroscopic observations of blue horizontal branch (HB) stars (i.e., HB stars hotter than the RR Lyrae instability strip) suggest that a large fraction of blue HB stars are second-generation stars formed with high helium abundances. In this paper, we test this scenario by using recent photometric and spectroscopic data together with theoretical evolutionary computations for different Y values. Comparing the photometric data with the theoretically derived color-magnitude diagrams, we find that the bulk of the blue HB stars in M4 have ΔY ≲ 0.01 with respect to the cluster's red HB stars (i.e., HB stars cooler than the RR Lyrae strip)—a result which is corroborated by comparison with spectroscopically derived gravities and temperatures, which also favor little He enhancement. However, the possible existence of a minority population on the blue HB of the cluster with a significant He enhancement level is also discussed.

  18. Constraints on helium enhancement in the globular cluster M4 (NGC 6121): The horizontal branch test

    International Nuclear Information System (INIS)

    Valcarce, A. A. R.; De Medeiros, J. R.; Catelan, M.; Alonso-García, J.; Cortés, C.

    2014-01-01

    Recent pieces of evidence have revealed that most, and possibly all, globular star clusters are composed of groups of stars that formed in multiple episodes with different chemical compositions. In this sense, it has also been argued that variations in the initial helium abundance (Y) from one population to the next are also the rule, rather than the exception. In the case of the metal-intermediate globular cluster M4 (NGC 6121), recent high-resolution spectroscopic observations of blue horizontal branch (HB) stars (i.e., HB stars hotter than the RR Lyrae instability strip) suggest that a large fraction of blue HB stars are second-generation stars formed with high helium abundances. In this paper, we test this scenario by using recent photometric and spectroscopic data together with theoretical evolutionary computations for different Y values. Comparing the photometric data with the theoretically derived color-magnitude diagrams, we find that the bulk of the blue HB stars in M4 have ΔY ≲ 0.01 with respect to the cluster's red HB stars (i.e., HB stars cooler than the RR Lyrae strip)—a result which is corroborated by comparison with spectroscopically derived gravities and temperatures, which also favor little He enhancement. However, the possible existence of a minority population on the blue HB of the cluster with a significant He enhancement level is also discussed.

  19. THE SURFACE-MEDIATED UNFOLDING KINETICS OF GLOBULAR PROTEINS IS DEPENDENT ON MOLECULAR WEIGHT AND TEMPERATURE

    Energy Technology Data Exchange (ETDEWEB)

    Patananan, A.N.; Goheen, S.C.

    2008-01-01

    The adsorption and unfolding pathways of proteins on rigid surfaces are essential in numerous complex processes associated with biomedical engineering, nanotechnology, and chromatography. It is now well accepted that the kinetics of unfolding are characterized by chemical and physical interactions dependent on protein deformability and structure, as well as environmental pH, temperature, and surface chemistry. Although this fundamental process has broad implications in medicine and industry, little is known about the mechanism because of the atomic lengths and rapid time scales involved. Therefore, the unfolding kinetics of myoglobin, β-glucosidase, and ovalbumin were investigated by adsorbing the globular proteins to non-porous cationic polymer beads. The protein fractions were adsorbed at different residence times (0, 9, 10, 20, and 30 min) at near-physiological conditions using a gradient elution system similar to that in high-performance liquid chromatography. The elution profi les and retention times were obtained by ultraviolet/visible spectrophotometry. A decrease in recovery was observed with time for almost all proteins and was attributed to irreversible protein unfolding on the non-porous surfaces. These data, and those of previous studies, fi t a positively increasing linear trend between percent unfolding after a fi xed (9 min) residence time (71.8%, 31.1%, and 32.1% of myoglobin, β-glucosidase, and ovalbumin, respectively) and molecular weight. Of all the proteins examined so far, only myoglobin deviated from this trend with higher than predicted unfolding rates. Myoglobin also exhibited an increase in retention time over a wide temperature range (0°C and 55°C, 4.39 min and 5.74 min, respectively) whereas ovalbumin and β-glucosidase did not. Further studies using a larger set of proteins are required to better understand the physiological and physiochemical implications of protein unfolding kinetics. This study confi rms that surface

  20. Image processing of globular clusters - Simulation for deconvolution tests (GlencoeSim)

    Science.gov (United States)

    Blazek, Martin; Pata, Petr

    2016-10-01

    This paper presents an algorithmic approach for efficiency tests of deconvolution algorithms in astronomic image processing. Due to the existence of noise in astronomical data there is no certainty that a mathematically exact result of stellar deconvolution exists and iterative or other methods such as aperture or PSF fitting photometry are commonly used. Iterative methods are important namely in the case of crowded fields (e.g., globular clusters). For tests of the efficiency of these iterative methods on various stellar fields, information about the real fluxes of the sources is essential. For this purpose a simulator of artificial images with crowded stellar fields provides initial information on source fluxes for a robust statistical comparison of various deconvolution methods. The "GlencoeSim" simulator and the algorithms presented in this paper consider various settings of Point-Spread Functions, noise types and spatial distributions, with the aim of producing as realistic an astronomical optical stellar image as possible.

  1. Toward the description of electrostatic interactions between globular proteins: potential of mean force in the primitive model.

    Science.gov (United States)

    Dahirel, Vincent; Jardat, Marie; Dufrêche, Jean-François; Turq, Pierre

    2007-09-07

    Monte Carlo simulations are used to calculate the exact potential of mean force between charged globular proteins in aqueous solution. The aim of the present paper is to study the influence of the ions of the added salt on the effective interaction between these nanoparticles. The charges of the model proteins, either identical or opposite, are either central or distributed on a discrete pattern. Contrarily to Poisson-Boltzmann predictions, attractive, and repulsive direct forces between proteins are not screened similarly. Moreover, it has been shown that the relative orientations of the charge patterns strongly influence salt-mediated interactions. More precisely, for short distances between the proteins, ions enhance the difference of the effective forces between (i) like-charged and oppositely charged proteins, (ii) attractive and repulsive relative orientations of the proteins, which may affect the selectivity of protein/protein recognition. Finally, such results observed with the simplest models are applied to a more elaborate one to demonstrate their generality.

  2. Cold-set globular protein gels: Interactions, structure and rheology as a function of protein concentration.

    NARCIS (Netherlands)

    Alting, A.C.; Hamer, R.J.; Kruif, de C.G.

    2003-01-01

    We identified the contribution of covalent and noncovalent interactions to the scaling behavior of the structural and rheological properties in a cold gelling protein system. The system we studied consisted of two types of whey protein aggregates, equal in size but different in the amount of

  3. Characterization of dry globular proteins and protein fibrils by synchrotron radiation vacuum UV circular dichroism

    DEFF Research Database (Denmark)

    Nesgaard, Lise W.; Hoffmann, Søren Vrønning; Andersen, Christian Beyschau

    2008-01-01

    Circular dichroism using synchrotron radiation (SRCD) can extend the spectral range down to approximately 130 nm for dry proteins, potentially providing new structural information. Using a selection of dried model proteins, including alpha-helical, beta-sheet, and mixed-structure proteins, we...... with previously published theoretical calculations related to pi-orbital transitions. We also show that drying does not lead to large changes in the secondary structure and does not induce orientational artifacts. In combination with principal component analysis, our SRCD data allow us to distinguish between two...... different types of protein fibrils, highlighting that bona fide fibrils formed by lysozyme are structurally more similar to the nonclassical fibrillar aggregates formed by the SerADan peptide than with the amyloid formed by alpha-synuclein. Thus, despite the lack of direct structural conclusions...

  4. New directions towards structure formation and stability of protein-rich foods from globular proteins

    NARCIS (Netherlands)

    Purwanti, N.; Goot, van der A.J.; Boom, R.M.; Vereijken, J.M.

    2010-01-01

    Concentrated protein-rich foods have strong potential to be developed in terms of health and well-being roles. Unfortunately, limitations in creating products with the rights texture and stability hinder the use of those products by consumers. Main reason is that the formation of micro- and

  5. THE SLUGGS SURVEY: NGC 3115, A CRITICAL TEST CASE FOR METALLICITY BIMODALITY IN GLOBULAR CLUSTER SYSTEMS

    Energy Technology Data Exchange (ETDEWEB)

    Brodie, Jean P.; Conroy, Charlie; Arnold, Jacob A.; Romanowsky, Aaron J. [University of California Observatories and Department of Astronomy and Astrophysics, University of California, Santa Cruz, CA 95064 (United States); Usher, Christopher; Forbes, Duncan A. [Centre for Astrophysics and Supercomputing, Swinburne University, Hawthorn, VIC 3122 (Australia); Strader, Jay, E-mail: brodie@ucolick.org [Department of Physics and Astronomy, Michigan State University, East Lansing, MI 48824 (United States)

    2012-11-10

    Due to its proximity (9 Mpc) and the strongly bimodal color distribution of its spectroscopically well-sampled globular cluster (GC) system, the early-type galaxy NGC 3115 provides one of the best available tests of whether the color bimodality widely observed in GC systems generally reflects a true metallicity bimodality. Color bimodality has alternatively been attributed to a strongly nonlinear color-metallicity relation reflecting the influence of hot horizontal-branch stars. Here, we couple Subaru Suprime-Cam gi photometry with Keck/DEIMOS spectroscopy to accurately measure GC colors and a CaT index that measures the Ca II triplet. We find the NGC 3115 GC system to be unambiguously bimodal in both color and the CaT index. Using simple stellar population models, we show that the CaT index is essentially unaffected by variations in horizontal-branch morphology over the range of metallicities relevant to GC systems (and is thus a robust indicator of metallicity) and confirm bimodality in the metallicity distribution. We assess the existing evidence for and against multiple metallicity subpopulations in early- and late-type galaxies and conclude that metallicity bi/multimodality is common. We briefly discuss how this fundamental characteristic links directly to the star formation and assembly histories of galaxies.

  6. THE SLUGGS SURVEY: NGC 3115, A CRITICAL TEST CASE FOR METALLICITY BIMODALITY IN GLOBULAR CLUSTER SYSTEMS

    International Nuclear Information System (INIS)

    Brodie, Jean P.; Conroy, Charlie; Arnold, Jacob A.; Romanowsky, Aaron J.; Usher, Christopher; Forbes, Duncan A.; Strader, Jay

    2012-01-01

    Due to its proximity (9 Mpc) and the strongly bimodal color distribution of its spectroscopically well-sampled globular cluster (GC) system, the early-type galaxy NGC 3115 provides one of the best available tests of whether the color bimodality widely observed in GC systems generally reflects a true metallicity bimodality. Color bimodality has alternatively been attributed to a strongly nonlinear color-metallicity relation reflecting the influence of hot horizontal-branch stars. Here, we couple Subaru Suprime-Cam gi photometry with Keck/DEIMOS spectroscopy to accurately measure GC colors and a CaT index that measures the Ca II triplet. We find the NGC 3115 GC system to be unambiguously bimodal in both color and the CaT index. Using simple stellar population models, we show that the CaT index is essentially unaffected by variations in horizontal-branch morphology over the range of metallicities relevant to GC systems (and is thus a robust indicator of metallicity) and confirm bimodality in the metallicity distribution. We assess the existing evidence for and against multiple metallicity subpopulations in early- and late-type galaxies and conclude that metallicity bi/multimodality is common. We briefly discuss how this fundamental characteristic links directly to the star formation and assembly histories of galaxies.

  7. Conformational and functional analysis of the C-terminal globular head of the reovirus cell attachment protein.

    Science.gov (United States)

    Duncan, R; Horne, D; Strong, J E; Leone, G; Pon, R T; Yeung, M C; Lee, P W

    1991-06-01

    We have been investigating structure-function relationships in the reovirus cell attachment protein sigma 1 using various deletion mutants and protease analysis. In the present study, a series of deletion mutants were constructed which lacked 90, 44, 30, 12, or 4 amino acids from the C-terminus of the 455-amino acid-long reovirus type 3 (T3) sigma 1 protein. The full-length and truncated sigma 1 proteins were expressed in an in vitro transcription/translation system and assayed for L cell binding activity. It was found that the removal of as few as four amino acids from the C-terminus drastically affected the cell binding function of the sigma 1 protein. The C-terminal-truncated proteins were further characterized using trypsin, chymotrypsin, and monoclonal and polyclonal antibodies. Our results indicated that the C-terminal portions of the mutant proteins were misfolded, leading to a loss in cell binding function. The N-terminal fibrous tail of the proteins was unaffected by the deletions as was sigma 1 oligomerization, further illustrating the discrete structural and functional roles of the N- and C-terminal domains of sigma 1. In an attempt to identify smaller, functional peptides, full-length sigma 1 expressed in vitro was digested with trypsin and subsequently with chymotrypsin under various conditions. The results clearly demonstrated the highly stable nature of the C-terminal globular head of sigma 1, even when separated from the N-terminal fibrous tail. We concluded that: (1) the C-terminal globular head of sigma 1 exists as a compact, protease-resistant oligomeric structure; (2) an intact C-terminus is required for proper head folding and generation of the conformationally dependent cell binding domain.

  8. A single cysteine post-translational oxidation suffices to compromise globular proteins kinetic stability and promote amyloid formation

    Directory of Open Access Journals (Sweden)

    Patrizia Marinelli

    2018-04-01

    Full Text Available Oxidatively modified forms of proteins accumulate during aging. Oxidized protein conformers might act as intermediates in the formation of amyloids in age-related disorders. However, it is not known whether this amyloidogenic conversion requires an extensive protein oxidative damage or it can be promoted just by a discrete, localized post-translational modification of certain residues. Here, we demonstrate that the irreversible oxidation of a single free Cys suffices to severely perturb the folding energy landscape of a stable globular protein, compromise its kinetic stability, and lead to the formation of amyloids under physiological conditions. Experiments and simulations converge to indicate that this specific oxidation-promoted protein aggregation requires only local unfolding. Indeed, a large scale analysis indicates that many cellular proteins are at risk of undergoing this kind of deleterious transition; explaining how oxidative stress can impact cell proteostasis and subsequently lead to the onset of pathological states. Keywords: Protein oxidation, Protein misfolding, Protein aggregation, Oxidative stress, Post-translational modification

  9. Front-face fluorescence spectroscopy study of globular proteins in emulsions: displacement of BSA by a nonionic surfactant.

    Science.gov (United States)

    Rampon, V; Genot, C; Riaublanc, A; Anton, M; Axelos, M A V; McClements, D J

    2003-04-23

    The displacement of a globular protein (bovine serum albumin, BSA) from the surface of oil droplets in concentrated oil-in-water emulsions by a nonionic surfactant (polyoxyethylene sorbitan monolauarate, Tween 20) was studied using front-face fluorescence spectroscopy (FFFS). This method relies on measurement of the change in intensity (I(MAX)) and wavelength (lambda(MAX)) of the maximum in the tryptophan emission spectrum. A series of oil-in-water emulsions (21 wt % n-hexadecane, 0.22 wt % BSA, pH 7.0) containing different molar ratios of Tween 20 to BSA (R = 0-131) were prepared. As the surfactant concentration was increased, the protein was progressively displaced from the droplet surfaces. At R > or = 66, the protein was completely displaced from the droplet surfaces. There was an increase in both I(MAX) and lambda(MAX) with increasing Tween 20 concentration up to R = 66, which correlated with the increase in the ratio of nonadsorbed to adsorbed protein. In contrast, there was a decrease in I(MAX) and lambda(MAX) with Tween 20 concentration in protein solutions and for R > or = 66 in the emulsions, which was attributed to binding of the surfactant to the protein. This study shows that FFFS is a powerful technique for nondestructively providing information about the interfacial composition of droplets in concentrated protein-stabilized emulsions in situ. Nevertheless, in general the suitability of the technique may also depend on protein type and the nature of the physicochemical matrix surrounding the proteins.

  10. Predicting the activity coefficients of free-solvent for concentrated globular protein solutions using independently determined physical parameters.

    Directory of Open Access Journals (Sweden)

    Devin W McBride

    Full Text Available The activity coefficient is largely considered an empirical parameter that was traditionally introduced to correct the non-ideality observed in thermodynamic systems such as osmotic pressure. Here, the activity coefficient of free-solvent is related to physically realistic parameters and a mathematical expression is developed to directly predict the activity coefficients of free-solvent, for aqueous protein solutions up to near-saturation concentrations. The model is based on the free-solvent model, which has previously been shown to provide excellent prediction of the osmotic pressure of concentrated and crowded globular proteins in aqueous solutions up to near-saturation concentrations. Thus, this model uses only the independently determined, physically realizable quantities: mole fraction, solvent accessible surface area, and ion binding, in its prediction. Predictions are presented for the activity coefficients of free-solvent for near-saturated protein solutions containing either bovine serum albumin or hemoglobin. As a verification step, the predictability of the model for the activity coefficient of sucrose solutions was evaluated. The predicted activity coefficients of free-solvent are compared to the calculated activity coefficients of free-solvent based on osmotic pressure data. It is observed that the predicted activity coefficients are increasingly dependent on the solute-solvent parameters as the protein concentration increases to near-saturation concentrations.

  11. Biological assessment of neonicotinoids imidacloprid and its major metabolites for potentially human health using globular proteins as a model.

    Science.gov (United States)

    Ding, Fei; Peng, Wei

    2015-06-01

    The assessment of biological activities of imidacloprid and its two major metabolites, namely 6-chloronicotinic acid and 2-imidazolidone for nontarget organism, by employing essentially functional biomacromolecules, albumin and hemoglobin as a potentially model with the use of circular dichroism (CD), fluorescence, extrinsic 8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence as well as molecular modeling is the theme of this work. By dint of CD spectra and synchronous fluorescence, it was clear that the orderly weak interactions between amino acid residues within globular proteins were disturbed by imidacloprid, and this event led to marginally alterations or self-regulations of protein conformation so as to lodge imidacloprid more tightly. Both steady state and time-resolved fluorescence suggested that the fluorescence of Trp residues in proteins was quenched after the presence of imidacloprid, corresponding to noncovalent protein-imidacloprid complexes formation and, the reaction belongs to moderate association (K=1.888/1.614×10(4)M(-1) for albumin/hemoglobin-imidacloprid, respectively), hydrogen bonds and π stacking performed a vital role in stabilizing the complexes, as derived from thermodynamic analysis and molecular modeling. With the aid of hydrophobic ANS experiments, subdomain IIA and α1β2 interface of albumin and hemoglobin, respectively, were found to be preserved high-affinity for imidacloprid. These results ties in with the subsequently molecular modeling laying imidacloprid in the Sudlow's site I and close to Trp-213 residue on albumin, while settling down B/Trp-37 residue nearby in hemoglobin, and these conclusions further confirmed by site-directed mutagenesis and molecular dynamics simulation. But, at the same time, several crucial noncovalent bonds came from other amino acid residues, e.g. Arg-194 and Arg-198 (albumin) and B/Arg-40, B/Asp-99 and B/Asn-102 (hemoglobin) cannot be ignored completely. Based on the comparative studies of

  12. Proteolytic activities of kiwifruit actinidin (Actinidia deliciosa cv. Hayward) on different fibrous and globular proteins: a comparative study of actinidin with papain.

    Science.gov (United States)

    Chalabi, Maryam; Khademi, Fatemeh; Yarani, Reza; Mostafaie, Ali

    2014-04-01

    Actinidin, a member of the papain-like family of cysteine proteases, is abundant in kiwifruit. To date, a few studies have been provided to investigate the proteolytic activity and substrate specificity of actinidin on native proteins. Herein, the proteolytic activity of actinidin was compared to papain on several different fibrous and globular proteins under neutral, acidic and basic conditions. The digested samples were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and densitometry to assess the proteolytic effect. Furthermore, the levels of free amino nitrogen (FAN) of the treated samples were determined using the ninhydrin colorimetric method. The findings showed that actinidin has no or limited proteolytic effect on globular proteins such as immunoglobulins including sheep IgG, rabbit IgG, chicken IgY and fish IgM, bovine serum albumin (BSA), lipid transfer protein (LTP), and whey proteins (α-lactalbumin and β-lactoglobulin) compared to papain. In contrast to globular proteins, actinidin could hydrolyze collagen and fibrinogen perfectly at neutral and mild basic pHs. Moreover, this enzyme could digest pure α-casein and major subunits of micellar casein especially in acidic pHs. Taken together, the data indicated that actinidin has narrow substrate specificity with the highest enzymatic activity for the collagen and fibrinogen substrates. The results describe the actinidin as a mild plant protease useful for many special applications such as cell isolation from different tissues and some food industries as a mixture formula with other relevant proteases.

  13. TESTING STELLAR POPULATION SYNTHESIS MODELS WITH SLOAN DIGITAL SKY SURVEY COLORS OF M31's GLOBULAR CLUSTERS

    International Nuclear Information System (INIS)

    Peacock, Mark B.; Zepf, Stephen E.; Maccarone, Thomas J.; Kundu, Arunav

    2011-01-01

    Accurate stellar population synthesis models are vital in understanding the properties and formation histories of galaxies. In order to calibrate and test the reliability of these models, they are often compared with observations of star clusters. However, relatively little work has compared these models in the ugriz filters, despite the recent widespread use of this filter set. In this paper, we compare the integrated colors of globular clusters in the Sloan Digital Sky Survey (SDSS) with those predicted from commonly used simple stellar population (SSP) models. The colors are based on SDSS observations of M31's clusters and provide the largest population of star clusters with accurate photometry available from the survey. As such, it is a unique sample with which to compare SSP models with SDSS observations. From this work, we identify a significant offset between the SSP models and the clusters' g - r colors, with the models predicting colors which are too red by g - r ∼ 0.1. This finding is consistent with previous observations of luminous red galaxies in the SDSS, which show a similar discrepancy. The identification of this offset in globular clusters suggests that it is very unlikely to be due to a minority population of young stars. The recently updated SSP model of Maraston and Stroembaeck better represents the observed g - r colors. This model is based on the empirical MILES stellar library, rather than theoretical libraries, suggesting an explanation for the g - r discrepancy.

  14. Protein selectivity with immobilized metal ion-tacn sorbents: chromatographic studies with human serum proteins and several other globular proteins.

    Science.gov (United States)

    Jiang, W; Graham, B; Spiccia, L; Hearn, M T

    1998-01-01

    The chromatographic selectivity of the immobilized chelate system, 1,4,7-triazocyclononane (tacn), complexed with the borderline metal ions Cu2+, Cr3+, Mn2+, Co2+, Zn2+, and Ni2+ has been investigated with hen egg white lysozyme, horse heart cytochrome c, and horse skeletal muscle myoglobin, as well as proteins present in partially fractionated preparations of human plasma. The effects of ionic strength and pH of the loading and elution buffers on protein selectivities of these new immobilized metal ion affinity chromatographic (IMAC) systems have been examined. The results confirm that immobilized Mn;pl-tacn sorbents exhibit a novel type of IMAC behavior with proteins. In particular, the chromatographic properties of these immobilized M(n+)-tacn ligand systems were significantly different compared to the IMAC behavior observed with other types of immobilized tri- and tetradentate chelating ligands, such as iminodiacetic acid, O-phosphoserine, or nitrilotriacetic acid, when complexed with borderline metal ions. The experimental results have consequently been evaluated in terms of the additional contributions to the interactive processes mediated by effects other than solely the conventional lone pair Lewis soft acid-Lewis soft base coordination interactions, typically found for the IMAC of proteins with borderline and soft metal ions, such as Cu2+ or Ni2+.

  15. Self-assembly dynamics for the transition of a globular aggregate to a fibril network of lysozyme proteins via a coarse-grained Monte Carlo simulation

    Directory of Open Access Journals (Sweden)

    R. B. Pandey

    2015-09-01

    Full Text Available The self-organizing dynamics of lysozymes (an amyloid protein with 148 residues with different numbers of protein chains, Nc = 1,5,10, and 15 (concentration 0.004 – 0.063 is studied by a coarse-grained Monte Carlo simulation with knowledge-based residue-residue interactions. The dynamics of an isolated lysozyme (Nc = 1 is ultra-slow (quasi-static at low temperatures and becomes diffusive asymptotically on raising the temperature. In contrast, the presence of interacting proteins leads to concentration induced protein diffusion at low temperatures and concentration-tempering sub-diffusion at high temperatures. Variation of the radius of gyration of the protein with temperature shows a systematic transition from a globular structure (at low T to a random coil (high T conformation when the proteins are isolated. The crossover from globular to random coil becomes sharper upon increasing the protein concentration (i.e. with Nc = 5,10, with larger Rg at higher temperatures and concentration; Rg becomes smaller on adding more protein chains (e.g. Nc = 15 a non-monotonic response to protein concentration. Analysis of the structure factor (S(q provides an estimate of the effective dimension (D ≥ 3, globular conformation at low temperature, and D ∼ 1.7, random coil, at high temperatures of the isolated protein. With many interacting proteins, the morphology of the self-assembly varies with scale, i.e. at the low temperature (T = 0.015, D ∼ 2.9 on the scale comparable to the radius of gyration of the protein, and D ∼ 2.3 at the large scale over the entire sample. The global network of fibrils appears at high temperature (T = 0.021 with D ∼ 1.7 (i.e. a random coil morphology at large scale involving tenuous distribution of micro-globules (at small scales.

  16. Calorimetric and spectroscopic properties of small globular proteins (bovine serum albumin, hemoglobin) after free radical generation

    International Nuclear Information System (INIS)

    Farkas, N.; Belagyi, J.; Lorinczy, D.

    2003-01-01

    Mild oxidation of -SH-containing proteins (serum albumin, hemoglobin) by Ce(IV)-ions in the presence of the spin trap phenyl-tert-butylnitrone (PBN) resulted in the appearance of strongly immobilized nitroxide free radicals which evidences the formation of thiyl radicals on the thiol site of the proteins. In hydroxyl free radical generating system a fraction of strongly immobilized nitroxide radicals was also detected in these proteins, which implies that the oxidation of a fraction of the thiol groups was also involved in the free radical reaction. According to the differential scanning calorimetry (DSC) experiments the melting processes of the proteins were calorimetrically irreversible, therefore the two-state kinetic model was used to evaluate the experiments. The results support the view that site-specific interaction of SH-containing proteins with hydroxyl and thiyl free radicals is able to modify the internal dynamics of proteins and affect the conformation of large molecules

  17. Stability of globular proteins in H2O and D2O

    NARCIS (Netherlands)

    Efimova, Y. M.; Haemers, S.; Wierczinski, B.; Norde, W.; van Well, A. A.

    2007-01-01

    In several experimental techniques D2O rather then H2O is often used as a solvent for proteins. Concerning the influence of the solvent on the stability of the proteins, contradicting results have been reported in literature. In this paper the influence of H2O-D2O solvent substitution on the

  18. Stability of globular proteins in H2O and in D2O

    NARCIS (Netherlands)

    Efimova, Y.M.; Haemers, S.; Wierczinsky, B.; Norde, W.; Well, van A.A.

    2007-01-01

    In several experimental techniques D2O rather then H2O is often used as a solvent for proteins. Concerning the influence of the solvent on the stability of the proteins, contradicting results have been reported in literature. In this paper the influence of H2O-D2O solvent substitution on the

  19. Study of the hydration of globular proteins by broad NMR lines method

    Energy Technology Data Exchange (ETDEWEB)

    Blicharska, B [Uniwersytet Jagiellonski, Krakow (Poland). Instytut Fizyki

    1973-01-01

    Spectra of proteins and polypeptides obtained by means of a NMR broad line spectrometer consist of broad and thin lines. These broad and thin lines are attributed to proteins and to water absorbed on the surfaces of proteins respectively. The behaviour of the thin line in the spectra of lyophilizated albumin of the egg white has been studied in the temperature range from -42 to 20/sup 0/C. The amount of water has been found by the simple method of weighing and has been equal about 7% of the total weight. It has been found that the water absorbed on the surface of the lyophilizated proteins gives a thinner line in comparison to the water absorbed on molecules of proteins in water solutions and that the correlation time is about 10/sup 3/ times greater.

  20. Testing lowered isothermal models with direct N-body simulations of globular clusters - II. Multimass models

    Science.gov (United States)

    Peuten, M.; Zocchi, A.; Gieles, M.; Hénault-Brunet, V.

    2017-09-01

    Lowered isothermal models, such as the multimass Michie-King models, have been successful in describing observational data of globular clusters. In this study, we assess whether such models are able to describe the phase space properties of evolutionary N-body models. We compare the multimass models as implemented in limepy (Gieles & Zocchi) to N-body models of star clusters with different retention fractions for the black holes and neutron stars evolving in a tidal field. We find that multimass models successfully reproduce the density and velocity dispersion profiles of the different mass components in all evolutionary phases and for different remnants retention. We further use these results to study the evolution of global model parameters. We find that over the lifetime of clusters, radial anisotropy gradually evolves from the low- to the high-mass components and we identify features in the properties of observable stars that are indicative of the presence of stellar-mass black holes. We find that the model velocity scale depends on mass as m-δ, with δ ≃ 0.5 for almost all models, but the dependence of central velocity dispersion on m can be shallower, depending on the dark remnant content, and agrees well with that of the N-body models. The reported model parameters, and correlations amongst them, can be used as theoretical priors when fitting these types of mass models to observational data.

  1. Structural features that predict real-value fluctuations of globular proteins.

    Science.gov (United States)

    Jamroz, Michal; Kolinski, Andrzej; Kihara, Daisuke

    2012-05-01

    It is crucial to consider dynamics for understanding the biological function of proteins. We used a large number of molecular dynamics (MD) trajectories of nonhomologous proteins as references and examined static structural features of proteins that are most relevant to fluctuations. We examined correlation of individual structural features with fluctuations and further investigated effective combinations of features for predicting the real value of residue fluctuations using the support vector regression (SVR). It was found that some structural features have higher correlation than crystallographic B-factors with fluctuations observed in MD trajectories. Moreover, SVR that uses combinations of static structural features showed accurate prediction of fluctuations with an average Pearson's correlation coefficient of 0.669 and a root mean square error of 1.04 Å. This correlation coefficient is higher than the one observed in predictions by the Gaussian network model (GNM). An advantage of the developed method over the GNMs is that the former predicts the real value of fluctuation. The results help improve our understanding of relationships between protein structure and fluctuation. Furthermore, the developed method provides a convienient practial way to predict fluctuations of proteins using easily computed static structural features of proteins. Copyright © 2012 Wiley Periodicals, Inc.

  2. Front-face fluorescence spectroscopy study of globular proteins in emulsions: influence of droplet flocculation.

    Science.gov (United States)

    Rampon, V; Genot, C; Riaublanc, A; Anton, M; Axelos, M A V; McClements, D J

    2003-04-23

    Measurement of the intensity (I(MAX)) and/or wavelength (lambda(MAX)) of the maximum in the tryptophan (TRP) emission spectrum using front-face fluorescence spectroscopy (FFFS) can be used to provide information about the molecular environment of proteins in nondiluted emulsions. Many protein-stabilized emulsions in the food industry are flocculated, and therefore, we examined the influence of droplet flocculation on FFFS. Stock oil-in-water emulsions stabilized by bovine serum albumin were prepared by high-pressure valve homogenization (30 wt % n-hexadecane, 0.35 wt % BSA, pH 7). These emulsions were used to create model systems with different degrees of droplet flocculation, either by changing the pH, adding surfactant, or adding xanthan. Emulsions (21 wt % n-hexadecane, 0.22 wt % BSA) with different pH (5 and 7) and molar ratios of Tween 20 to BSA (R = 0-131) were prepared by dilution of the stock emulsion. As the surfactant concentration was increased, the protein was displaced from the droplet surfaces, which caused an increase in both I(MAX) and lambda(MAX), because of the change in TRP environment. The dependence of I(MAX) and lambda(MAX) on surfactant concentration followed a similar pattern in emulsions that were initially flocculated (pH 5) and nonflocculated (pH 7). Relatively small changes in FFFS emission spectra were observed in emulsions (21 wt % n-hexadecane, 0.22 wt % BSA, pH 7) with different levels of depletion flocculation induced by adding xanthan. These results suggested that droplet flocculation did not have a major impact on FFFS. This study shows that FFFS is a powerful technique for nondestructively providing information about the molecular environment of proteins in concentrated and flocculated protein-stabilized emulsions. Nevertheless, in general the suitability of the technique may also depend on protein type and the nature of the physicochemical matrix surrounding the proteins.

  3. In vitro biosynthesis of globular proteins by murine splenic lymphocytes: effect of serum components as supplement

    International Nuclear Information System (INIS)

    Tandon, H.K.L.; Pandey, A.K.; Singh, L.N.

    1991-01-01

    Studies on replacement of foetal calf serum (FCS) with precipitable protein, non precipitable protein, dialysable and non dialysable components of the FCS in media for the growth and proliferation of murine splenic rat lymphocytes have revealed that the whole serum could be completely replaced by either of the components without any appreciable deleterious effect on the mitogenic response but none of these components could offer optimum immune response. These findings establish a covalent association of whole FCS for synthesis and secretion of immunologically important pulsed proteins in terms of turnover rate and quantification by FPLC and suggest an important and yet undefined role of FCS in the process of immunoglobulin synthesis. (author). 10 refs., 2 figs., 1 tab

  4. Zaccai neutron resilience and site-specific hydration dynamics in a globular protein

    Energy Technology Data Exchange (ETDEWEB)

    Miao, Yinglong [Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Hong, Liang [Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Yi, Zheng [Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Smith, Jeremy C. [Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2013-07-16

    A discussion is presented of contributions of the Zaccai group to the understanding of flexibility in biological macromolecules using dynamic neutron scattering. The concept of resilience as introduced by Zaccai is discussed and investigated using molecular dynamics simulation on camphor-bound cytochrome P450. The resilience of hydrophilic residues is found to be more strongly affected by hydration than that of hydrophobic counterparts. The hydration-induced softening of protein propagates from the surface into the dry core. Furthermore, buried hydrophilic residues behave more like those exposed on the protein surface, and are different from their hydrophobic counterparts.

  5. On the effect of hydrostatic pressure on the conformational stability of globular proteins.

    Science.gov (United States)

    Graziano, Giuseppe

    2015-12-01

    The model developed for cold denaturation (Graziano, PCCP 2010, 12, 14245-14252) is extended to rationalize the dependence of protein conformational stability upon hydrostatic pressure, at room temperature. A pressure- volume work is associated with the process of cavity creation for the need to enlarge the liquid volume against hydrostatic pressure. This contribution destabilizes the native state that has a molecular volume slightly larger than the denatured state due to voids existing in the protein core. Therefore, there is a hydrostatic pressure value at which the pressure-volume contribution plus the conformational entropy loss of the polypeptide chain are able to overwhelm the stabilizing gain in translational entropy of water molecules, due to the decrease in water accessible surface area upon folding, causing denaturation. © 2015 Wiley Periodicals, Inc.

  6. Does the Sauerbrey equation hold true for binding of peptides and globular proteins to a QCM?

    Directory of Open Access Journals (Sweden)

    Jean E. Sohna Sohna

    2016-12-01

    Full Text Available The term ‘microbalance’ arose after Sauerbrey showed a mass per unit area dependence on sensor frequency change for thin, solid films on a QCM. Others have extrapolated this relationship to interactions with biological ‘soft’ matter using acoustic wave devices. We rigorously examined the relationship between QCM frequency change and the molecular weight of protein and peptide analytes on a RAP♦id 4™ system using more than 120 individual assays. A series of amino acid, peptides and proteins with molecular weight from 372 to 150,000 Da constituting a molecular weight ladder were biotinylated with a target biotin/protein ratio close to one to minimize avidity effects. Analyte concentration and contact time were chosen so as to attain near saturation of an anti-biotin antibody surface. The series resistance and resonant frequency changes (dF and dR arising from a 5-parameter fit of the imaginary component of the impedance signal were analysed, giving a linear relationship (R2 = 0.98 between frequency response and analyte molecular weight, even down to level of a single amino-acid. As predicted by theory, there was also a linear relationship between the changes in density and viscosity of the liquid in contact with the sensor and both dF and dR. The resistance and resonance frequency changes recorded for mixtures of deuterium oxide and glycerol were the sum of changes induced by each individual liquid. Hence the Sauerbrey equation for mass per unit area dependence of QCM signal does hold true for peptides and proteins in a liquid. Keywords: Calibration, Quartz crystal microbalance, Thickness shear mode, Biosensor, Microfluidics, Resonant acoustic profiling

  7. Photophysical studies of PET based acridinedione dyes with globular protein: Bovine serum albumin

    International Nuclear Information System (INIS)

    Rajendran, Kumaran; Perumal, Ramamurthy

    2010-01-01

    Interaction of acridinedione dyes with model transport proteins, bovine serum albumin (BSA) in aqueous solution were investigated by fluorescence spectral studies. A fluorescence enhancement was observed on the addition of BSA to photoinduced electron transfer (PET) based acridinedione dyes, which posses C 6 H 4 (p-OCH 3 ) in the 9th position of the basic acridinedione ring. On the contrary, the addition of BSA to non-PET based acridinedione dyes with methyl or phenyl substitution in the 9th position does not result in any fluorescence enhancement. The enhancement in the fluorescence intensity is attributed to the suppression of PET process through space between -OCH 3 group and the acridinedione moiety is elucidated by steady state fluorescence measurements. The fluorescence anisotropy value (r) of 0.40 reveals that the motion of the dye molecule is highly constrained and is largely confined to the rigid microenvironment of the protein molecule. The binding constant (K) was found to be in the order of 6.0x10 3 [M] -1 , which implies the existence of hydrophobic interaction between the PET based dye and BSA. Time resolved fluorescence lifetime measurements reveal that the PET based acridinedione dye preferably binds in the hydrophobic interior of BSA.

  8. Pulsed laser study of excited states of aromatic molecules absorbed in globular proteins

    International Nuclear Information System (INIS)

    Cooper, M.; Thomas, J.K.

    1977-01-01

    Pyrene and several derivatives of pyrene such as pyrene sulfonic acid, and pyrene butyric acid were incorporated into bovine serum albumin (BSA) in aqueous solution. The pyrene chromophore was subsequently excited by a pulse of uv light (lambda = 3471 A) from a Q switched frequency doubled ruby laser. The lifetime of the pyrene excited singlet and triplet states were monitored by time resolved spectrophotometry. Various molecules, such as O 2 and I - , dissolved in the aqueous phase, diffused into the protein and quenched pyrene excited states. The rates of these reactions were followed under a variety of conditions such as pH and temperature and in the presence of inert additives. The rates of pyrene excited-state quenching were often considerably smaller than the rates observed in simple solutions. A comparison of the rates in the protein and homogeneous solutions gives information on the factors such as temperature, charge, and pH that control the movement of small molecules in and into BSA

  9. Acid-Induced Cold Gelation of Globular Proteins: Effects of Protein Aggregate Characteristics and Disulfide Bonding on Rheological Properties

    NARCIS (Netherlands)

    Alting, A.C.; Weijers, M.; Hoog, E.H.A. de; Pijpekamp, A.M. van de; Cohen Stuart, M.A.; Hamer, R.J.; Kruif, C.G. de; Visschers, R.W.

    2004-01-01

    The process of cold gelation of ovalbumin and the properties of the resulting cold-set gels were compared to those of whey protein isolate. Under the chosen heating conditions, most protein was organized in aggregates. For both protein preparations, the aggregates consisted of covalently linked

  10. Structure-volume relationships: singular volume effects produced by cupric ion-globular protein interaction.

    Science.gov (United States)

    Katz, S; Shinaberry, G; Heck, E L; Squire, W

    1980-08-05

    The nature of the volume isotherms produced by the coordination of Cu(II) with ovalbumin and bovine serum albumin differs substantially from the adsorption isotherms produced by these systems. Whereas there was increased binding of Cu(II) associated with a pH increase from pH 5.3 to pH 7.4, the volume isotherms for these systems did not exhibit this type of pH dependence. The volume changes were determined at 30.0 +/- 0.001 degrees C with microdilatometers which could be read to 0.01 muL. The binding isotherms for ovalbumin at pH 5.3 and 7.4 and for bovine serum albumin at pH 5.3 was resolved by a Scatchard plot to yield the appropriate thermodynamic parameters. An algorithm was derived to calculate the distribution of the individual PMi complexes, i.e., PMi-1 + M in equilibrium (Ki) PMi where i equals 1, 2, 3, ..., n moles of cation, M, bound per mole of protein, P, for the above systems. The volume isotherms were then resolved in terms of the constituent delta Vi terms, i.e., the volume change produced by the formation of the individual PMi complexes. These values were verified by an independent graphical differentiation procedure. The coordination of Cu(II) to BSA at pH 7.4 produced a cooperative adsorption isotherm which was not amenable to a Scatchard analysis. The resultant anomalous volume isotherm was resolved into a component related to Cu(II)-site interaction and a negative volume effect attributable to a conformational change induced by complex formation. This structural transition which occurs at physiological pH may constitute a control mechanism for regulating the serum level of Cu(II) and possibly other divalent ions.

  11. Larger red-shift in optical emissions obtained from the thin films of globular proteins (BSA, lysozyme) – polyelectrolyte (PAA) complexes

    Energy Technology Data Exchange (ETDEWEB)

    Talukdar, Hrishikesh [Physical Sciences Division, Institute of Advanced Study in Science and Technology, Vigyan Path, Paschim Boragaon, Garchuk, Guwahati 781035, Assam (India); Kundu, Sarathi, E-mail: sarathi.kundu@gmail.com [Physical Sciences Division, Institute of Advanced Study in Science and Technology, Vigyan Path, Paschim Boragaon, Garchuk, Guwahati 781035, Assam (India); Basu, Saibal [Solid State Physics Division, Bhabha Atomic Research Centre, Mumbai 400 085 (India)

    2016-09-30

    Graphical abstract: Thin films of protein-polyelectrolyte complexes show larger red-shift in optical emission. - Highlights: • Globular proteins (lysozyme and BSA) and polyelectrolyte (sodium polyacrylic acid) are used to form protein-polyelectrolyte complexes (PPC). • Larger red-shift in optical emission is obtained from the thin films of PPC. • Red-shift is not obtained from the solution of PPC and pure protein thin films. • Larger red-shift from PPC films is due to the energy dissipation as non-radiative form through interactions with nearby atoms. • Red-shift in optical emission is independent on the thickness of the PPC film. - Abstract: Globular proteins (lysozyme and BSA) and polyelectrolyte (sodium polyacrylic acid) are used to form protein-polyelectrolyte complexes (PPC). Out-of-plane structures of ≈30–60 nm thick PPC films and their surface morphologies have been studied by using X-ray reflectivity and atomic force microscopy, whereas optical behaviors of PPC and protein conformations have been studied by using UV–vis, photoluminescence and FTIR spectroscopy respectively. Our study reveals that thin films of PPC show a larger red-shift of 23 and 16 nm in the optical emissions in comparison to that of pure protein whereas bulk PPC show a small blue-shift of ≈3 nm. A small amount of peak-shift is found to occur due to the heat treatment or concentration variation of the polyelectrolyte/protein in bulk solution but cannot produce such film thickness independent larger red-shift. Position of the emission peak remains nearly unchanged with the film thickness. Mechanism for such larger red-shift has been proposed.

  12. Urine protein electrophoresis test

    Science.gov (United States)

    Urine protein electrophoresis; UPEP; Multiple myeloma - UPEP; Waldenström macroglobulinemia - UPEP; Amyloidosis - UPEP ... special paper and apply an electric current. The proteins move and form visible bands. These reveal the ...

  13. The central globular domain of the nucleocapsid protein of human immunodeficiency virus type 1 is critical for virion structure and infectivity.

    Science.gov (United States)

    Ottmann, M; Gabus, C; Darlix, J L

    1995-03-01

    The nucleocapsid protein NCp7 of human immunodeficiency virus type 1 (HIV-1) is a 72-amino-acid peptide containing two CCHC-type zinc fingers linked by a short basic sequence, 29RAPRKKG35, which is conserved in HIV-1 and simian immunodeficiency virus. The complete three-dimensional structure of NCp7 has been determined by 1H-nuclear magnetic resonance spectroscopy (N. Morellet, H. de Rocquigny, Y. Mely, N. Jullian, H. Demene, M. Ottmann, D. Gerard, J. L. Darlix, M. C. Fournié-Zaluski, and B. P. Roques, J. Mol. Biol. 235:287-301, 1994) and revealed a central globular domain where the two zinc fingers are brought in close proximity by the RAPRKKG linker. To examine the role of this globular structure and more precisely of the RAPRKKG linker in virion structure and infectivity, we generated HIV-1 DNA mutants in the RAPRKK sequence of NCp7 and analyzed the mutant virions produced by transfected cells. Mutations that probably alter the structure of NCp7 structure led to the formation of very poorly infectious virus (A30P) or noninfectious virus (P31L and R32G). In addition, the P31L mutant did not contain detectable amounts of reverse transcriptase and had an immature core morphology, as determined by electron microscopy. On the other hand, mutations changing the basic nature of NCp7 had poor effect. R29S had a wild-type phenotype, and the replacement of 32RKK34 by SSS (S3 mutant) resulted in a decrease by no more than 100-fold of the virus titer. These results clearly show that the RAPRKKG linker contains residues that are critical for virion structure and infectivity.

  14. Separation of both fibrous and globular proteins on the basis of molecular weight using high-performance size exclusion chromatography.

    Science.gov (United States)

    Barden, J A

    1983-11-01

    A high-performance size exclusion liquid chromatographic system has been used to separate proteins with different shapes solely on the basis of their molecular weights. After the effects of ionic and hydrophobic interactions with the stationary phase have been overcome, protein elution is normally governed by their effective size in solution. Conditions are described under which proteins, with isoelectric points within the normal operating pH range of the columns, are eluted independent of their Stokes' radii. Even fibrous proteins with axial ratios of 50 elute according to their known molecular weights over the range 2000-2,000,000.

  15. Chemical denaturation of globular proteins at the air/water interface: an x-ray and neutron reflectometry study

    International Nuclear Information System (INIS)

    Perriman, A.W.; Henderson, M.J.; White, J.W.

    2003-01-01

    Full text: X-ray and neutron reflectometry has been used to probe the equilibrium surface structure of hen egg white lysozyme (lysozyme) and bovine β -lactoglobulin (β -lactoglobulin) under denaturing conditions at the air-water interface. This was achieved by performing experiments on 10 mg mL -1 protein solutions containing increasing concentrations of the chemical denaturant guanidinium hydrochloride (G.HCl). For solutions containing no G.HCl, the surface structure of the proteins was represented by a two-layer model with total thicknesses of 48 Angstroms and 38 Angstroms for lysozyme and β -lactoglobulin, respectively. The total volume of a single protein molecule and the associated water molecules was evaluated to be approximately 45 (0.3) nm 3 for lysozyme, and 60 (0.3) nm 3 for β-lactoglobulin. The thickness dimensions and the total volumes compared favourably with the crystal dimensions of 45 x 30 x 30 Angstroms (40.5 nm 3 ),1 and 36 x 36 x 36 Angstroms (47 nm 3 ) 2 for lysozyme and β -lactoglobulin, respectively. This comparison suggests that when no denaturant was present, the structures of lysozyme and β -lactoglobulin were near to their native conformations at the air-water interface. The response to the presence of the chemical denaturant was different for each protein. The surface layer of β-lactoglobulin expanded at very low concentrations (0.2 mol dm -3 ) of G.HCl. In contrast, the lysozyme layer contracted. At higher concentrations, unfolding of both the proteins led to the formation of a third diffuse layer. In general, lysozyme appeared to be less responsive to the chemical denaturant, which is most likely a result of the higher disulfide content of lysozyme. A protocol allowing quantitative thermodynamic analysis of the contribution from the air-water interface to the chemical denaturation of a protein was developed

  16. Optimal definition of inter-residual contact in globular proteins based on pairwise interaction energy calculations, its robustness, and applications.

    Science.gov (United States)

    Fačkovec, Boris; Vondrášek, Jiří

    2012-10-25

    Although a contact is an essential measurement for the topology as well as strength of non-covalent interactions in biomolecules and their complexes, there is no general agreement in the definition of this feature. Most of the definitions work with simple geometric criteria which do not fully reflect the energy content or ability of the biomolecular building blocks to arrange their environment. We offer a reasonable solution to this problem by distinguishing between "productive" and "non-productive" contacts based on their interaction energy strength and properties. We have proposed a method which converts the protein topology into a contact map that represents interactions with statistically significant high interaction energies. We do not prove that these contacts are exclusively stabilizing, but they represent a gateway to thermodynamically important rather than geometry-based contacts. The process is based on protein fragmentation and calculation of interaction energies using the OPLS force field and relies on pairwise additivity of amino acid interactions. Our approach integrates the treatment of different types of interactions, avoiding the problems resulting from different contributions to the overall stability and the different effect of the environment. The first applications on a set of homologous proteins have shown the usefulness of this classification for a sound estimate of protein stability.

  17. Optimal Definition of Inter-Residual Contact in Globular Proteins Based on Pairwise Interaction Energy Calculations, Its Robustness, and Applications

    Czech Academy of Sciences Publication Activity Database

    Fačkovec, Boris; Vondrášek, Jiří

    2012-01-01

    Roč. 116, č. 42 (2012), s. 12651-12660 ISSN 1520-6106 R&D Projects: GA ČR GAP208/10/0725; GA MŠk(CZ) LH11020 Institutional support: RVO:61388963 Keywords : egg-white lysozyme * force-field * 3-dimensional structure * thermophilic proteins * thermal-stability * mutant Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 3.607, year: 2012

  18. Globular clusters - Fads and fallacies

    International Nuclear Information System (INIS)

    White, R.E.

    1991-01-01

    The types of globular clusters observed in the Milky Way Galaxy are described together with their known characteristics, with special attention given to correcting the erroneous statements made earlier about globular clusters. Among these are the following statements: the Galaxy is surrounded by many hundreds of globular clusters; all globular clusters are located toward the Galactic center, all globular clusters are metal poor and move about the Galaxy in highly elliptical paths; all globular clusters contain RR Lyrae-type variable stars, and the RR Lyrae stars found outside of globulars have come from cluster dissolution or ejection; all of the stars in a given cluster were born at the same time and have the same chemical composition; X-ray globulars are powered by central black holes; and the luminosity functions for globular clusters are well defined and well determined. Consideration is given to the fact that globular clusters in the Magellanic Clouds differ from those in the Milky Way by their age distribution and that the globulars of the SMC differ from those of the LMC

  19. Globular Clusters - Guides to Galaxies

    CERN Document Server

    Richtler, Tom; Joint ESO-FONDAP Workshop on Globular Clusters

    2009-01-01

    The principal question of whether and how globular clusters can contribute to a better understanding of galaxy formation and evolution is perhaps the main driving force behind the overall endeavour of studying globular cluster systems. Naturally, this splits up into many individual problems. The objective of the Joint ESO-FONDAP Workshop on Globular Clusters - Guides to Galaxies was to bring together researchers, both observational and theoretical, to present and discuss the most recent results. Topics covered in these proceedings are: internal dynamics of globular clusters and interaction with host galaxies (tidal tails, evolution of cluster masses), accretion of globular clusters, detailed descriptions of nearby cluster systems, ultracompact dwarfs, formations of massive clusters in mergers and elsewhere, the ACS Virgo survey, galaxy formation and globular clusters, dynamics and kinematics of globular cluster systems and dark matter-related problems. With its wide coverage of the topic, this book constitute...

  20. Globular clusters, old and young

    International Nuclear Information System (INIS)

    Samus', N.N.

    1984-01-01

    The problem of similarity of and difference in the globular and scattered star clusters is considered. Star clusters in astronomy are related either to globular or to scattered ones according to the structure of Hertzsprung-Russell diagram constructed for star clusters, but not according to the appearance. The qlobular clusters in the Galaxy are composed of giants and subgiants, which testifies to the old age of the globular clusters. The Globular clusters in the Magellanic clouds are classified into ''red'' ones - similar to the globular clusters of the Galaxy, and ''blue'' ones - similar to them in appearance but differing extremely by the star composition and so by the age. The old star clusters are suggested to be called globular ones, while another name (''populous'', for example) is suggested to be used for other clusters similar to globular ones only in appearance

  1. THE METALLICITY BIMODALITY OF GLOBULAR CLUSTER SYSTEMS: A TEST OF GALAXY ASSEMBLY AND OF THE EVOLUTION OF THE GALAXY MASS-METALLICITY RELATION

    International Nuclear Information System (INIS)

    Tonini, Chiara

    2013-01-01

    We build a theoretical model to study the origin of the globular cluster metallicity bimodality in the hierarchical galaxy assembly scenario. The model is based on empirical relations such as the galaxy mass-metallicity relation [O/H]-M star as a function of redshift, and on the observed galaxy stellar mass function up to redshift z ∼ 4. We make use of the theoretical merger rates as a function of mass and redshift from the Millennium simulation to build galaxy merger trees. We derive a new galaxy [Fe/H]-M star relation as a function of redshift, and by assuming that globular clusters share the metallicity of their original parent galaxy at the time of their formation, we populate the merger tree with globular clusters. We perform a series of Monte Carlo simulations of the galaxy hierarchical assembly, and study the properties of the final globular cluster population as a function of galaxy mass, assembly and star formation history, and under different assumptions for the evolution of the galaxy mass-metallicity relation. The main results and predictions of the model are the following. (1) The hierarchical clustering scenario naturally predicts a metallicity bimodality in the galaxy globular cluster population, where the metal-rich subpopulation is composed of globular clusters formed in the galaxy main progenitor around redshift z ∼ 2, and the metal-poor subpopulation is composed of clusters accreted from satellites, and formed at redshifts z ∼ 3-4. (2) The model reproduces the observed relations by Peng et al. for the metallicities of the metal-rich and metal-poor globular cluster subpopulations as a function of galaxy mass; the positions of the metal-poor and metal-rich peaks depend exclusively on the evolution of the galaxy mass-metallicity relation and the [O/Fe], both of which can be constrained by this method. In particular, we find that the galaxy [O/Fe] evolves linearly with redshift from a value of ∼0.5 at redshift z ∼ 4 to a value of ∼0.1 at

  2. MEASURING DETAILED CHEMICAL ABUNDANCES FROM CO-ADDED MEDIUM-RESOLUTION SPECTRA. I. TESTS USING MILKY WAY DWARF SPHEROIDAL GALAXIES AND GLOBULAR CLUSTERS

    International Nuclear Information System (INIS)

    Yang Lei; Peng, Eric W.; Kirby, Evan N.; Guhathakurta, Puragra; Cheng, Lucy

    2013-01-01

    The ability to measure metallicities and α-element abundances in individual red giant branch (RGB) stars using medium-resolution spectra (R ≈ 6000) is a valuable tool for deciphering the nature of Milky Way dwarf satellites and the history of the Galactic halo. Extending such studies to more distant systems like Andromeda is beyond the ability of the current generation of telescopes, but by co-adding the spectra of similar stars, we can attain the necessary signal-to-noise ratio (S/N) to make detailed abundance measurements. In this paper, we present a method to determine metallicities and α-element abundances using the co-addition of medium-resolution spectra. We test the method of spectral co-addition using high-S/N spectra of more than 1300 RGB stars from Milky Way globular clusters and dwarf spheroidal galaxies obtained with the Keck II telescope/DEIMOS spectrograph. We group similar stars using photometric criteria and compare the weighted ensemble average abundances ([Fe/H], [Mg/Fe], [Si/Fe], [Ca/Fe], and [Ti/Fe]) of individual stars in each group with the measurements made on the corresponding co-added spectrum. We find a high level of agreement between the two methods, which permits us to apply this co-added spectra technique to more distant RGB stars, like stars in the M31 satellite galaxies. This paper outlines our spectral co-addition and abundance measurement methodology and describes the potential biases in making these measurements.

  3. Measuring Detailed Chemical Abundances from Co-added Medium-resolution Spectra. I. Tests Using Milky Way Dwarf Spheroidal Galaxies and Globular Clusters

    Science.gov (United States)

    Yang, Lei; Kirby, Evan N.; Guhathakurta, Puragra; Peng, Eric W.; Cheng, Lucy

    2013-05-01

    The ability to measure metallicities and α-element abundances in individual red giant branch (RGB) stars using medium-resolution spectra (R ≈ 6000) is a valuable tool for deciphering the nature of Milky Way dwarf satellites and the history of the Galactic halo. Extending such studies to more distant systems like Andromeda is beyond the ability of the current generation of telescopes, but by co-adding the spectra of similar stars, we can attain the necessary signal-to-noise ratio (S/N) to make detailed abundance measurements. In this paper, we present a method to determine metallicities and α-element abundances using the co-addition of medium-resolution spectra. We test the method of spectral co-addition using high-S/N spectra of more than 1300 RGB stars from Milky Way globular clusters and dwarf spheroidal galaxies obtained with the Keck II telescope/DEIMOS spectrograph. We group similar stars using photometric criteria and compare the weighted ensemble average abundances ([Fe/H], [Mg/Fe], [Si/Fe], [Ca/Fe], and [Ti/Fe]) of individual stars in each group with the measurements made on the corresponding co-added spectrum. We find a high level of agreement between the two methods, which permits us to apply this co-added spectra technique to more distant RGB stars, like stars in the M31 satellite galaxies. This paper outlines our spectral co-addition and abundance measurement methodology and describes the potential biases in making these measurements. Data herein were obtained at the W. M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California, and NASA. The Observatory was made possible by the generous financial support of the W. M. Keck Foundation.

  4. Search for optical millisecond pulsars in globular clusters

    International Nuclear Information System (INIS)

    Middleditch, J.H.; Imamura, J.N.; Steiman-Cameron, T.Y.

    1988-01-01

    A search for millisecond optical pulsars in several bright, compact globular clusters was conducted. The sample included M28, and the X-ray clusters 47 Tuc, NGC 6441, NGC 6624, M22, and M15. The globular cluster M28 contains the recently discovered 327 Hz radio pulsar. Upper limits of 4 sigma to pulsed emission of (1-20) solar luminosities were found for the globular clusters tested, and 0.3 solar luminosity for the M28 pulsar for frequencies up to 500 Hz. 8 references

  5. Parathyroid hormone-related protein blood test

    Science.gov (United States)

    ... ency/article/003691.htm Parathyroid hormone-related protein blood test To use the sharing features on this page, ... measures the level of a hormone in the blood, called parathyroid hormone-related protein. How the Test is Performed A blood sample is needed . How ...

  6. Optics of globular photonic crystals

    International Nuclear Information System (INIS)

    Gorelik, V S

    2007-01-01

    The results of experimental and theoretical studies of the optical properties of globular photonic crystals - new physical objects having a crystal structure with the lattice period exceeding considerably the atomic size, are presented. As globular photonic crystals, artificial opal matrices consisting of close-packed silica globules of diameter ∼200 nm were used. The reflection spectra of these objects characterising the parameters of photonic bands existing in these crystals in the visible spectral region are presented. The idealised models of the energy band structure of photonic crystals investigated in the review give analytic dispersion dependences for the group velocity and the effective photon mass in a globular photonic crystal. The characteristics of secondary emission excited in globular photonic crystals by monochromatic and broadband radiation are presented. The results of investigations of single-photon-excited delayed scattering of light observed in globular photonic crystals exposed to cw UV radiation and radiation from a repetitively pulsed copper vapour laser are presented. The possibilities of using globular photonic crystals as active media for lasing in different spectral regions are considered. It is proposed to use globular photonic crystals as sensitive sensors in optoelectronic devices for molecular analysis of organic and inorganic materials by the modern methods of laser spectroscopy. The results of experimental studies of spontaneous and stimulated globular scattering of light are discussed. The conditions for observing resonance and two-photon-excited delayed scattering of light are found. The possibility of accumulation and localisation of the laser radiation energy inside a globular photonic crystal is reported. (review)

  7. Relativistic Binaries in Globular Clusters

    Directory of Open Access Journals (Sweden)

    Matthew J. Benacquista

    2013-03-01

    Full Text Available Galactic globular clusters are old, dense star systems typically containing 10^4 – 10^6 stars. As an old population of stars, globular clusters contain many collapsed and degenerate objects. As a dense population of stars, globular clusters are the scene of many interesting close dynamical interactions between stars. These dynamical interactions can alter the evolution of individual stars and can produce tight binary systems containing one or two compact objects. In this review, we discuss theoretical models of globular cluster evolution and binary evolution, techniques for simulating this evolution that leads to relativistic binaries, and current and possible future observational evidence for this population. Our discussion of globular cluster evolution will focus on the processes that boost the production of tight binary systems and the subsequent interaction of these binaries that can alter the properties of both bodies and can lead to exotic objects. Direct N-body integrations and Fokker–Planck simulations of the evolution of globular clusters that incorporate tidal interactions and lead to predictions of relativistic binary populations are also discussed. We discuss the current observational evidence for cataclysmic variables, millisecond pulsars, and low-mass X-ray binaries as well as possible future detection of relativistic binaries with gravitational radiation.

  8. IAG ring test animal proteins 2015

    NARCIS (Netherlands)

    Raamsdonk, van L.W.D.; Rhee, van de N.E.; Scholtens-Toma, I.M.J.; Prins, T.W.; Vliege, J.J.M.; Pinckaers, V.G.Z.

    2015-01-01

    A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The organizer of the ring test was RIKILT - Wageningen UR, The

  9. Globular clusters and galaxy halos

    International Nuclear Information System (INIS)

    Van Den Bergh, S.

    1984-01-01

    Using semipartial correlation coefficients and bootstrap techniques, a study is made of the important features of globular clusters with respect to the total number of galaxy clusters and dependence of specific galaxy cluster on parent galaxy type, cluster radii, luminosity functions and cluster ellipticity. It is shown that the ellipticity of LMC clusters correlates significantly with cluster luminosity functions, but not with cluster age. The cluter luminosity value above which globulars are noticeably flattened may differ by a factor of about 100 from galaxy to galaxy. Both in the Galaxy and in M31 globulars with small core radii have a Gaussian distribution over luminosity, whereas clusters with large core radii do not. In the cluster systems surrounding the Galaxy, M31 and NGC 5128 the mean radii of globular clusters was found to increase with the distance from the nucleus. Central galaxies in rich clusters have much higher values for specific globular cluster frequency than do other cluster ellipticals, suggesting that such central galaxies must already have been different from normal ellipticals at the time they were formed

  10. IAG ring test animal proteins 2014

    NARCIS (Netherlands)

    Raamsdonk, van L.W.D.; Pinckaers, V.G.Z.; Scholtens-Toma, I.M.J.; Prins, T.W.; Voet, van der H.; Vliege, J.J.M.

    2014-01-01

    A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG – International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The aim of the ring study was to provide the participants

  11. IAG ring test animal proteins 2013

    NARCIS (Netherlands)

    Raamsdonk, van L.W.D.; Pinckaers, V.G.Z.; Scholtens-Toma, I.M.J.; Prins, T.W.; Vliege, J.J.M.

    2013-01-01

    A ring test was organized for the detection of animal proteins in animal feed by microscopy in the framework of the annual ring tests of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy. The organizer of the the ring study was to provide the

  12. IAG ring test animal proteins 2016

    NARCIS (Netherlands)

    Raamsdonk, van L.W.D.; Rhee, van de N.E.; Scholtens-Toma, I.M.J.; Prins, T.W.; Vliege, J.J.M.; Pinckaers, V.G.Z.

    2016-01-01

    The annual ring test for the detection of animal proteins in animal feed of the IAG - International Association for Feeding stuff Analysis, Section Feeding stuff Microscopy was organized by RIKILT - Wageningen UR, The Netherlands. The aim of the ring study was to provide the participants information

  13. Effects of non-covalent interactions with 5-O-caffeoylquinic acid (chlorogenic acid) on the heat denaturation and solubility of globular proteins

    NARCIS (Netherlands)

    Prigent, S.V.E.; Gruppen, H.; Visser, A.J.W.G.; Koningsveld, G.A. van; Jong, G.A.H. de; Voragen, A.G.J.

    2003-01-01

    The non-covalent interactions between the monomeric phenolic compound chlorogenic acid (5-CQA) and bovine serum albumin (BSA), lysozyme, and α-lactalbumin were characterized, and their effect on protein properties was examined. 5-CQA had a low affinity for all three proteins, and these interactions

  14. The WAGGS project - I. The WiFeS Atlas of Galactic Globular cluster Spectra

    Science.gov (United States)

    Usher, Christopher; Pastorello, Nicola; Bellstedt, Sabine; Alabi, Adebusola; Cerulo, Pierluigi; Chevalier, Leonie; Fraser-McKelvie, Amelia; Penny, Samantha; Foster, Caroline; McDermid, Richard M.; Schiavon, Ricardo P.; Villaume, Alexa

    2017-07-01

    We present the WiFeS Atlas of Galactic Globular cluster Spectra, a library of integrated spectra of Milky Way and Local Group globular clusters. We used the WiFeS integral field spectrograph on the Australian National University 2.3 m telescope to observe the central regions of 64 Milky Way globular clusters and 22 globular clusters hosted by the Milky Way's low-mass satellite galaxies. The spectra have wider wavelength coverage (3300-9050 Å) and higher spectral resolution (R = 6800) than existing spectral libraries of Milky Way globular clusters. By including Large and Small Magellanic Cloud star clusters, we extend the coverage of parameter space of existing libraries towards young and intermediate ages. While testing stellar population synthesis models and analysis techniques is the main aim of this library, the observations may also further our understanding of the stellar populations of Local Group globular clusters and make possible the direct comparison of extragalactic globular cluster integrated light observations with well-understood globular clusters in the Milky Way. The integrated spectra are publicly available via the project website.

  15. Protein in Urine: MedlinePlus Lab Test Information

    Science.gov (United States)

    ... this page: https://medlineplus.gov/labtests/proteininurine.html Protein in Urine To use the sharing features on this page, please enable JavaScript. What is a Protein in Urine Test? A protein in urine test ...

  16. Speckle imaging of globular clusters

    International Nuclear Information System (INIS)

    Sams, B.J. III

    1990-01-01

    Speckle imaging is a powerful tool for high resolution astronomy. Its application to the core regions of globular clusters produces high resolution stellar maps of the bright stars, but is unable to image the faint stars which are most reliable dynamical indicators. The limits on resolving these faint, extended objects are physical, not algorithmic, and cannot be overcome using speckle. High resolution maps may be useful for resolving multicomponent stellar systems in the cluster centers. 30 refs

  17. Globular Clusters for Faint Galaxies

    Science.gov (United States)

    Kohler, Susanna

    2017-07-01

    The origin of ultra-diffuse galaxies (UDGs) has posed a long-standing mystery for astronomers. New observations of several of these faint giants with the Hubble Space Telescope are now lending support to one theory.Faint-Galaxy MysteryHubble images of Dragonfly 44 (top) and DFX1 (bottom). The right panels show the data with greater contrast and extended objects masked. [van Dokkum et al. 2017]UDGs large, extremely faint spheroidal objects were first discovered in the Virgo galaxy cluster roughly three decades ago. Modern telescope capabilities have resulted in many more discoveries of similar faint galaxies in recent years, suggesting that they are a much more common phenomenon than we originally thought.Despite the many observations, UDGs still pose a number of unanswered questions. Chief among them: what are UDGs? Why are these objects the size of normal galaxies, yet so dim? There are two primary models that explain UDGs:UDGs were originally small galaxies, hence their low luminosity. Tidal interactions then puffed them up to the large size we observe today.UDGs are effectively failed galaxies. They formed the same way as normal galaxies of their large size, but something truncated their star formation early, preventing them from gaining the brightness that we would expect for galaxies of their size.Now a team of scientists led by Pieter van Dokkum (Yale University) has made some intriguing observations with Hubble that lend weight to one of these models.Globulars observed in 16 Coma-cluster UDGs by Hubble. The top right panel shows the galaxy identifications. The top left panel shows the derived number of globular clusters in each galaxy. [van Dokkum et al. 2017]Globulars GaloreVan Dokkum and collaborators imaged two UDGs with Hubble: Dragonfly 44 and DFX1, both located in the Coma galaxy cluster. These faint galaxies are both smooth and elongated, with no obvious irregular features, spiral arms, star-forming regions, or other indications of tidal interactions

  18. ω-Turn: a novel β-turn mimic in globular proteins stabilized by main-chain to side-chain C−H···O interaction.

    Science.gov (United States)

    Dhar, Jesmita; Chakrabarti, Pinak; Saini, Harpreet; Raghava, Gajendra Pal Singh; Kishore, Raghuvansh

    2015-02-01

    Mimicry of structural motifs is a common feature in proteins. The 10-membered hydrogen-bonded ring involving the main-chain C − O in a β-turn can be formed using a side-chain carbonyl group leading to Asx-turn. We show that the N − H component of hydrogen bond can be replaced by a C(γ) -H group in the side chain, culminating in a nonconventional C − H···O interaction. Because of its shape this β-turn mimic is designated as ω-turn, which is found to occur ∼ three times per 100 residues. Three residues (i to i + 2) constitute the turn with the C − H···O interaction occurring between the terminal residues, constraining the torsion angles ϕi + 1, ψi + 1, ϕi + 2 and χ'1(i + 2) (using the interacting C(γ) atom). Based on these angles there are two types of ω-turns, each of which can be further divided into two groups. C(β) -branched side-chains, and Met and Gln have high propensities to occur at i + 2; for the last two residues the carbonyl oxygen may participate in an additional interaction involving the S and amino group, respectively. With Cys occupying the i + 1 position, such turns are found in the metal-binding sites. N-linked glycosylation occurs at the consensus pattern Asn-Xaa-Ser/Thr; with Thr at i + 2, the sequence can adopt the secondary structure of a ω-turn, which may be the recognition site for protein modification. Location between two β-strands is the most common occurrence in protein tertiary structure, and being generally exposed ω-turn may constitute the antigenic determinant site. It is a stable scaffold and may be used in protein engineering and peptide design. © 2014 Wiley Periodicals, Inc.

  19. 21 CFR 862.1630 - Protein (fractionation) test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Protein (fractionation) test system. 862.1630... Systems § 862.1630 Protein (fractionation) test system. (a) Identification. A protein (fractionation) test system is a device intended to measure protein fractions in blood, urine, cerebrospinal fluid, and other...

  20. REVIEW: Optics of globular photonic crystals

    Science.gov (United States)

    Gorelik, V. S.

    2007-05-01

    The results of experimental and theoretical studies of the optical properties of globular photonic crystals - new physical objects having a crystal structure with the lattice period exceeding considerably the atomic size, are presented. As globular photonic crystals, artificial opal matrices consisting of close-packed silica globules of diameter ~200 nm were used. The reflection spectra of these objects characterising the parameters of photonic bands existing in these crystals in the visible spectral region are presented. The idealised models of the energy band structure of photonic crystals investigated in the review give analytic dispersion dependences for the group velocity and the effective photon mass in a globular photonic crystal. The characteristics of secondary emission excited in globular photonic crystals by monochromatic and broadband radiation are presented. The results of investigations of single-photon-excited delayed scattering of light observed in globular photonic crystals exposed to cw UV radiation and radiation from a repetitively pulsed copper vapour laser are presented. The possibilities of using globular photonic crystals as active media for lasing in different spectral regions are considered. It is proposed to use globular photonic crystals as sensitive sensors in optoelectronic devices for molecular analysis of organic and inorganic materials by the modern methods of laser spectroscopy. The results of experimental studies of spontaneous and stimulated globular scattering of light are discussed. The conditions for observing resonance and two-photon-excited delayed scattering of light are found. The possibility of accumulation and localisation of the laser radiation energy inside a globular photonic crystal is reported.

  1. Systematic main sequence photometry of globular cluster stars for age determination

    International Nuclear Information System (INIS)

    Alcaino, G.; Liller, W.

    1984-01-01

    The individual photometric study of the coeval stars in globular clusters presents one of the best observational tests of the stellar evolution theory. Our own globular cluster system provides fundamental clues to the dynamical and chemical evolutionary history of the galaxy, and the study of their ages give a lower limit to the age of the galaxy as well as to that of the universe. The authors have undertaken a systematic research program, and discuss the ages deduced by fitting main sequence photometry to theoretical isochrones of six galactic globular clusters: M4, M22, M30, NGC 288, NGC 3201 and NGC 6397. (Auth.)

  2. EVIDENCE FOR AN ACCRETION ORIGIN FOR THE OUTER HALO GLOBULAR CLUSTER SYSTEM OF M31

    International Nuclear Information System (INIS)

    Mackey, A. D.; Huxor, A. P.; Ferguson, A. M. N.; Irwin, M. J.; Chapman, S. C.; Tanvir, N. R.; McConnachie, A. W.; Ibata, R. A.; Lewis, G. F.

    2010-01-01

    We use a sample of newly discovered globular clusters from the Pan-Andromeda Archaeological Survey (PAndAS) in combination with previously cataloged objects to map the spatial distribution of globular clusters in the M31 halo. At projected radii beyond ∼30 kpc, where large coherent stellar streams are readily distinguished in the field, there is a striking correlation between these features and the positions of the globular clusters. Adopting a simple Monte Carlo approach, we test the significance of this association by computing the probability that it could be due to the chance alignment of globular clusters smoothly distributed in the M31 halo. We find that the likelihood of this possibility is low, below 1%, and conclude that the observed spatial coincidence between globular clusters and multiple tidal debris streams in the outer halo of M31 reflects a genuine physical association. Our results imply that the majority of the remote globular cluster system of M31 has been assembled as a consequence of the accretion of cluster-bearing satellite galaxies. This constitutes the most direct evidence to date that the outer halo globular cluster populations in some galaxies are largely accreted.

  3. Protein-Based Urine Test Predicts Kidney Transplant Outcomes

    Science.gov (United States)

    ... News Releases News Release Thursday, August 22, 2013 Protein-based urine test predicts kidney transplant outcomes NIH- ... supporting development of noninvasive tests. Levels of a protein in the urine of kidney transplant recipients can ...

  4. TOWARDS THE CONCEPT OF GLOBULAR PEARLITE

    OpenAIRE

    A. G. Anisovich; M. K. Stepankova; A. A. Andrushevich

    2016-01-01

    Explanatory imprecisions of concept of globular pearlite and ferrite-carbide-mixture are considered. The need of concept binding of globular pearlite to specific grain with 0.8% carbon content is explained with the assistance of exemplary data obtained at the present metallographic equipment. The question of educational material presentation concerning the process of teaching of discipline «Materials and construction materials technology» is discussed in relation to the educational process of...

  5. Gamma-ray Emission from Globular Clusters

    Directory of Open Access Journals (Sweden)

    Pak-Hin T. Tam

    2016-03-01

    Full Text Available Over the last few years, the data obtained using the Large Area Telescope (LAT aboard the Fermi Gamma-ray Space Telescope has provided new insights on high-energy processes in globular clusters, particularly those involving compact objects such as MilliSecond Pulsars (MSPs. Gamma-ray emission in the 100 MeV to 10 GeV range has been detected from more than a dozen globular clusters in our galaxy, including 47 Tucanae and Terzan 5. Based on a sample of known gammaray globular clusters, the empirical relations between gamma-ray luminosity and properties of globular clusters such as their stellar encounter rate, metallicity, and possible optical and infrared photon energy densities, have been derived. The measured gamma-ray spectra are generally described by a power law with a cut-off at a few gigaelectronvolts. Together with the detection of pulsed γ-rays from two MSPs in two different globular clusters, such spectral signature lends support to the hypothesis that γ-rays from globular clusters represent collective curvature emission from magnetospheres of MSPs in the clusters. Alternative models, involving Inverse-Compton (IC emission of relativistic electrons that are accelerated close to MSPs or pulsar wind nebula shocks, have also been suggested. Observations at >100 GeV by using Fermi/LAT and atmospheric Cherenkov telescopes such as H.E.S.S.-II, MAGIC-II, VERITAS, and CTA will help to settle some questions unanswered by current data.

  6. Total Protein and Albumin/Globulin Ratio Test

    Science.gov (United States)

    ... Plasma Free Metanephrines Platelet Count Platelet Function Tests Pleural Fluid Analysis PML-RARA Porphyrin Tests Potassium Prealbumin ... of the various types of proteins in the liquid ( serum or plasma ) portion of the blood. Two ...

  7. The Blue Hook Populations of Massive Globular Clusters

    Science.gov (United States)

    Brown, Thomas

    2006-07-01

    Blue hook stars are a class of hot { 35,000 K} subluminous horizontal branch stars that have been recently discovered using HST ultraviolet images of the globular clusters omega Cen and NGC 2808. These stars occupy a region of the HR diagram that is unexplained by canonical stellar evolution theory. Using new theoretical evolutionary and atmospheric models, we have shown that the blue hook stars are very likely the progeny of stars that undergo extensive internal mixing during a late helium core flash on the white dwarf cooling curve. This "flash mixing" produces an enormous enhancement of the surface helium and carbon abundances, which suppresses the flux in the far ultraviolet. Although flash mixing is more likely to occur in stars that are born with high helium abundances, a high helium abundance, by itself, does not explain the presence of a blue hook population - flash mixing of the envelope is required. We propose ACS ultraviolet {SBC/F150LP and HRC/F250W} observations of the five additional globular clusters for which the presence of blue hook stars is suspected from longer wavelength observations. Like omega Cen and NGC 2808, these five targets are also among the most massive globular clusters, because less massive clusters show no evidence for blue hook stars. Because our targets span 1.5 dex in metallicity, we will be able to test our prediction that flash-mixing should be less drastic in metal-rich blue hook stars. In addition, our observations will test the hypothesis that blue hook stars only form in globular clusters massive enough to retain the helium-enriched ejecta from the first stellar generation. If this hypothesis is correct, then our observations will yield important constraints on the chemical evolution and early formation history in globular clusters, as well as the role of helium self-enrichment in producing blue horizontal branch morphologies and multiple main sequence turnoffs. Finally, our observations will provide new insight into the

  8. C-Reactive Protein (CRP) Test

    Science.gov (United States)

    ... can be caused by a number of different pathological conditions such as arthritis , lupus , or inflammatory bowel ... clinchem.org . Accessed October 2011. Lowry, F. (2010 March 23). CRP Test Guides Antibiotic Prescribing for Respiratory ...

  9. Dinamical properties of globular clusters: Primordial or evolutional?

    Science.gov (United States)

    Surdin, V. G.

    1995-04-01

    Some observable relations between globular cluster parameters appear as a result of dynamical evolution of the cluster system. These relations are inapplicable to the studies of the globular cluster origin

  10. Nova-driven winds in globular clusters

    International Nuclear Information System (INIS)

    Scott, E.H.; Durisen, R.H.

    1978-01-01

    Recent sensitive searches for Hα emission from ionized intracluster gas in globular clusters have set upper limits that conflict with theoretical predictions. We suggest that nova outbursts heat the gas, producing winds that resolve this discrepancy. The incidence of novae in globular clusters, the conversion of kinetic energy of the nova shell to thermal energy of the intracluster gas, and the characteristics of the resultant winds are discussed. Calculated emission from the nova-driven models does not conflict with any observations to date. Some suggestions are made concerning the most promising approaches for future detection of intracluster gas on the basis of these models. The possible relationship of nova-driven winds of globular cluster X-ray sources is also considered

  11. Reconstructing galaxy histories from globular clusters.

    Science.gov (United States)

    West, Michael J; Côté, Patrick; Marzke, Ronald O; Jordán, Andrés

    2004-01-01

    Nearly a century after the true nature of galaxies as distant 'island universes' was established, their origin and evolution remain great unsolved problems of modern astrophysics. One of the most promising ways to investigate galaxy formation is to study the ubiquitous globular star clusters that surround most galaxies. Globular clusters are compact groups of up to a few million stars. They generally formed early in the history of the Universe, but have survived the interactions and mergers that alter substantially their parent galaxies. Recent advances in our understanding of the globular cluster systems of the Milky Way and other galaxies point to a complex picture of galaxy genesis driven by cannibalism, collisions, bursts of star formation and other tumultuous events.

  12. TOWARDS THE CONCEPT OF GLOBULAR PEARLITE

    Directory of Open Access Journals (Sweden)

    A. G. Anisovich

    2016-01-01

    Full Text Available Explanatory imprecisions of concept of globular pearlite and ferrite-carbide-mixture are considered. The need of concept binding of globular pearlite to specific grain with 0.8% carbon content is explained with the assistance of exemplary data obtained at the present metallographic equipment. The question of educational material presentation concerning the process of teaching of discipline «Materials and construction materials technology» is discussed in relation to the educational process of technical universities, in particular, the Belarusian State Agrarian Technical University.

  13. Millisecond radio pulsars in globular clusters

    Science.gov (United States)

    Verbunt, Frank; Lewin, Walter H. G.; Van Paradijs, Jan

    1989-01-01

    It is shown that the number of millisecond radio pulsars, in globular clusters, should be larger than 100, applying the standard scenario that all the pulsars descend from low-mass X-ray binaries. Moreover, most of the pulsars are located in a small number of clusters. The prediction that Teran 5 and Liller 1 contain at least about a dozen millisecond radio pulsars each is made. The observations of millisecond radio pulsars in globular clusters to date, in particular the discovery of two millisecond radio pulsars in 47 Tuc, are in agreement with the standard scenario, in which the neutron star is spun up during the mass transfer phase.

  14. Shaping Globular Clusters with Black Holes

    Science.gov (United States)

    Kohler, Susanna

    2018-03-01

    How many black holes lurk within the dense environments of globular clusters, and how do these powerful objects shape the properties of the cluster around them? One such cluster, NGC 3201, is now helping us to answer these questions.Hunting Stellar-Mass Black HolesSince the detection of merging black-hole binaries by the Laser Interferometer Gravitational-Wave Observatory (LIGO), the dense environments of globular clusters have received increasing attention as potential birthplaces of these compact binary systems.The central region of the globular star cluster NGC 3201, as viewed by Hubble. The black hole is in orbit with the star marked by the blue circle. [NASA/ESA]In addition, more and more stellar-mass black-hole candidates have been observed within globular clusters, lurking in binary pairs with luminous, non-compact companions. The most recent of these detections, found in the globular cluster NGC 3201, stands alone as the first stellar-mass black hole candidate discovered via radial velocity observations: the black holes main-sequence companion gave away its presence via a telltale wobble.Now a team of scientists led by Kyle Kremer (CIERA and Northwestern University) is using models of this system to better understand the impact that black holes might have on their host clusters.A Model ClusterThe relationship between black holes and their host clusters is complicated. Though the cluster environment can determine the dynamical evolution of the black holes, the retention rate of black holes in a globular cluster (i.e., how many remain in the cluster when they are born as supernovae, rather than being kicked out during the explosion) influences how the host cluster evolves.Kremer and collaborators track this complex relationship by modeling the evolution of a cluster similar to NGC 3201 with a Monte Carlo code. The code incorporates physics relevant to the evolution of black holes and black-hole binaries in globular clusters, such as two-body relaxation

  15. Globular adiponectin ameliorates metabolic insulin resistance via AMPK-mediated restoration of microvascular insulin responses

    Science.gov (United States)

    Zhao, Lina; Fu, Zhuo; Wu, Jing; Aylor, Kevin W; Barrett, Eugene J; Cao, Wenhong; Liu, Zhenqi

    2015-01-01

    Abstract Hypoadiponectinaemia is closely associated with endothelial dysfunction and insulin resistance, and microvasculature plays a critical role in the regulation of insulin action in muscle. Here we tested whether adiponectin replenishment could improve metabolic insulin sensitivity in male rats fed a high-fat diet (HFD) via the modulation of microvascular insulin responses. Male Sprague–Dawley rats were fed either a HFD or low-fat diet (LFD) for 4 weeks. Small resistance artery myograph changes in tension, muscle microvascular recruitment and metabolic response to insulin were determined. Compared with rats fed a LFD, HFD feeding abolished the vasodilatory actions of globular adiponectin (gAd) and insulin on pre-constricted distal saphenous arteries. Pretreatment with gAd improved insulin responses in arterioles isolated from HFD rats, which was blocked by AMP-activated protein kinase (AMPK) inhibition. Similarly, HFD abolished microvascular responses to either gAd or insulin and decreased insulin-stimulated glucose disposal by ∼60%. However, supplementing gAd fully rescued insulin’s microvascular action and significantly improved the metabolic responses to insulin in HFD male rats and these actions were abolished by inhibition of either AMPK or nitric oxide production. We conclude that HFD induces vascular adiponectin and insulin resistance but gAd administration can restore vascular insulin responses and improve insulin’s metabolic action via an AMPK- and nitric oxide-dependent mechanism in male rats. Key points Adiponectin is an adipokine with anti-inflammatory and anti-diabetic properties. Hypoadiponectinaemia is closely associated with endothelial dysfunction and insulin resistance in obesity and diabetes. Insulin resistance is present in muscle microvasculature and this may contribute to decreased insulin delivery to, and action in, muscle. In this study we examined whether adiponectin ameliorates metabolic insulin resistance by affecting muscle

  16. Dityrosine, 3,4-Dihydroxyphenylalanine (DOPA), and radical formation from tyrosine residues on milk proteins with globular and flexible structures as a result of riboflavin-mediated photo-oxidation

    DEFF Research Database (Denmark)

    Dalsgaard, Trine Kastrup; Nielsen, Jacob Holm; Brown, Bronwyn

    2011-01-01

    Riboflavin-mediated photo-oxidative damage to protein Tyr residues has been examined to determine whether protein structure influences competing protein oxidation pathways in single proteins and protein mixtures. EPR studies resulted in the detection of Tyr-derived o-semiquione radicals, with thi......Riboflavin-mediated photo-oxidative damage to protein Tyr residues has been examined to determine whether protein structure influences competing protein oxidation pathways in single proteins and protein mixtures. EPR studies resulted in the detection of Tyr-derived o-semiquione radicals...

  17. Photometric studies of globular clusters in the Andromeda Nebula. Luminosity function for old globular clusters

    International Nuclear Information System (INIS)

    Sharov, A.S.; Lyutyj, V.M.

    1989-01-01

    The luminosity function for old globular clusters in M 31 is presented. The objects were selected according to their structural and photometric properties. At the usually accepted normal (Gaussian) distribution, the luminosity function is characterized by the following parameters: the mean magnitude, corrected for the extinction inside M 31, V-bar 0 =16 m ,38±0 m .08, and the absolute magnitude M-bar v =-8 m .29 assuming )m-M) v =23 m .67, standard deviation σ M v =1 m .16±0 m .08 and total object number N=300±17. Old globular clusters in M 31 are in the average about one magnitude more luminous then those in our Galaxy (M v ≅ -7 m .3). Intrinsic luminosity dispersions of globular clusters are nearly the same in both galaxies. Available data on globular clusters in the Local Group galaxies against the universality of globular luminosity function with identical parameters M v and σ M v

  18. BtcA, A class IA type III chaperone, interacts with the BteA N-terminal domain through a globular/non-globular mechanism.

    Directory of Open Access Journals (Sweden)

    Chen Guttman

    Full Text Available Bordetella pertussis, the etiological agent of "whooping cough" disease, utilizes the type III secretion system (T3SS to deliver a 69 kDa cytotoxic effector protein, BteA, directly into the host cells. As with other T3SS effectors, prior to its secretion BteA binds BtcA, a 13.9 kDa protein predicted to act as a T3SS class IA chaperone. While this interaction had been characterized for such effector-chaperone pairs in other pathogens, it has yet to be fully investigated in Bordetella. Here we provide the first biochemical proof that BtcA is indeed a class IA chaperone, responsible for the binding of BteA's N-terminal domain. We bring forth extensive evidence that BtcA binds its substrate effector through a dual-interface binding mechanism comprising of non-globular and bi-globular interactions at a moderate micromolar level binding affinity. We demonstrate that the non-globular interactions involve the first 31 N-terminal residues of BteA287 and their removal leads to destabilization of the effector-chaperone complex and lower binding affinities to BtcA. These findings represent an important first step towards a molecular understanding of BteA secretion and cell entry.

  19. Infrared dust emission from globular clusters

    International Nuclear Information System (INIS)

    Angeletti, L.; Capuzzo-Dolcetta, R.; Giannone, P.; Blanco, A.; Bussoletti, E.

    1982-01-01

    The implications of the presence of a central cloud in the cores of globular clusters were investigated recently. A possible mechanism of confinement of dust in the central region of our cluster models was also explored. The grain temperature and infrared emission have now been computed for rather realistic grain compositions. The grain components were assumed to be graphite and/or silicates. The central clouds turned out to be roughly isothermal. The wavelengths of maximum emission came out to be larger than 20 μm in all studied cases. An application of the theoretical results to five globular clusters showed that the predictable infrared emission for 47 Tuc, M4 and M22 should be detectable by means of present instrumentation aboard flying platforms. (author)

  20. Infrared dust emission from globular clusters

    Energy Technology Data Exchange (ETDEWEB)

    Angeletti, L; Capuzzo-Dolcetta, R; Giannone, P. (Rome Univ. (Italy). Osservatorio Astronomico); Blanco, A; Bussoletti, E [Lecce Univ. (Italy). Ist. di Fisica

    1982-05-01

    The implications of the presence of a central cloud in the cores of globular clusters were investigated recently. A possible mechanism of confinement of dust in the central region of our cluster models was also explored. The grain temperature and infrared emission have now been computed for rather realistic grain compositions. The grain components were assumed to be graphite and/or silicates. The central clouds turned out to be roughly isothermal. The wavelengths of maximum emission came out to be larger than 20 ..mu..m in all studied cases. An application of the theoretical results to five globular clusters showed that the predictable infrared emission for 47 Tuc, M4 and M22 should be detectable by means of present instrumentation aboard flying platforms.

  1. Chemical Abundances of Giants in Globular Clusters

    Science.gov (United States)

    Gratton, Raffaele G.; Bragaglia, Angela; Carretta, Eugenio; D'Orazi, Valentina; Lucatello, Sara

    A large fraction of stars form in clusters. According to a widespread paradigma, stellar clusters are prototypes of single stellar populations. According to this concept, they formed on a very short time scale, and all their stars share the same chemical composition. Recently it has been understood that massive stellar clusters (the globular clusters) rather host various stellar populations, characterized by different chemical composition: these stellar populations have also slightly different ages, stars of the second generations being formed from the ejecta of part of those of an earlier one. Furthermore, it is becoming clear that the efficiency of the process is quite low: many more stars formed within this process than currently present in the clusters. This implies that a significant, perhaps even dominant fraction of the ancient population of galaxies formed within the episodes that lead to formation the globular clusters.

  2. Dynamical Friction in Multi-component Evolving Globular Clusters

    Science.gov (United States)

    Alessandrini, Emiliano; Lanzoni, Barbara; Miocchi, Paolo; Ciotti, Luca; Ferraro, Francesco R.

    2014-11-01

    We use the Chandrasekhar formalism and direct N-body simulations to study the effect of dynamical friction on a test object only slightly more massive than the field stars, orbiting a spherically symmetric background of particles with a mass spectrum. The main goal is to verify whether the dynamical friction time (t DF) develops a non-monotonic radial dependence that could explain the bimodality of the blue straggler radial distributions observed in globular clusters. In these systems, in fact, relaxation effects lead to a mass and velocity radial segregation of the different mass components, so that mass-spectrum effects on t DF are expected to be dependent on radius. We find that in spite of the presence of different masses, t DF is always a monotonic function of radius, at all evolutionary times and independently of the initial concentration of the simulated cluster. This is because the radial dependence of t DF is largely dominated by the total mass density profile of the background stars (which is monotonically decreasing with radius). Hence, a progressive temporal erosion of the blue straggler star (BSS) population at larger and larger distances from the cluster center remains the simplest and the most likely explanation of the shape of the observed BSS radial distributions, as suggested in previous works. We also confirm the theoretical expectation that approximating a multi-mass globular cluster as made of (averaged) equal-mass stars can lead to significant overestimations of t DF within the half-mass radius.

  3. Blind Test of Physics-Based Prediction of Protein Structures

    Science.gov (United States)

    Shell, M. Scott; Ozkan, S. Banu; Voelz, Vincent; Wu, Guohong Albert; Dill, Ken A.

    2009-01-01

    We report here a multiprotein blind test of a computer method to predict native protein structures based solely on an all-atom physics-based force field. We use the AMBER 96 potential function with an implicit (GB/SA) model of solvation, combined with replica-exchange molecular-dynamics simulations. Coarse conformational sampling is performed using the zipping and assembly method (ZAM), an approach that is designed to mimic the putative physical routes of protein folding. ZAM was applied to the folding of six proteins, from 76 to 112 monomers in length, in CASP7, a community-wide blind test of protein structure prediction. Because these predictions have about the same level of accuracy as typical bioinformatics methods, and do not utilize information from databases of known native structures, this work opens up the possibility of predicting the structures of membrane proteins, synthetic peptides, or other foldable polymers, for which there is little prior knowledge of native structures. This approach may also be useful for predicting physical protein folding routes, non-native conformations, and other physical properties from amino acid sequences. PMID:19186130

  4. Close stellar encounters in globular clusters

    International Nuclear Information System (INIS)

    Bailyn, C.D.

    1989-01-01

    Stellar encounters are expected to produce a variety of interesting objects in the cores of globular clusters, either through the formation of binaries by tidal capture, or direct collisions. Here, I describe several attempts to observe the products of stellar encounters. In particular, the use of color maps has demonstrated the existence of a color gradient in the core of M15, which seems to be caused by a population of faint blue objects concentrated towards the cluster center. (author)

  5. Exploring the Internal Dynamics of Globular Clusters

    Science.gov (United States)

    Watkins, Laura L.; van der Marel, Roeland; Bellini, Andrea; Luetzgendorf, Nora; HSTPROMO Collaboration

    2018-01-01

    Exploring the Internal Dynamics of Globular ClustersThe formation histories and structural properties of globular clusters are imprinted on their internal dynamics. Energy equipartition results in velocity differences for stars of different mass, and leads to mass segregation, which results in different spatial distributions for stars of different mass. Intermediate-mass black holes significantly increase the velocity dispersions at the centres of clusters. By combining accurate measurements of their internal kinematics with state-of-the-art dynamical models, we can characterise both the velocity dispersion and mass profiles of clusters, tease apart the different effects, and understand how clusters may have formed and evolved.Using proper motions from the Hubble Space Telescope Proper Motion (HSTPROMO) Collaboration for a set of 22 Milky Way globular clusters, and our discrete dynamical modelling techniques designed to work with large, high-quality datasets, we are studying a variety of internal cluster properties. We will present the results of theoretical work on simulated clusters that demonstrates the efficacy of our approach, and preliminary results from application to real clusters.

  6. In vitro study of proteins surface activity by tritium probe

    International Nuclear Information System (INIS)

    Chernysheva, M.G.; Badun, G.A.

    2010-01-01

    A new technique for in vitro studies of biomacromolecules interactions, their adsorption at aqueous/organic liquid interfaces and distribution in the bulk of liquid/liquid systems was developed. The method includes (1) tritium labeling of biomolecules by tritium thermal activation method and (2) scintillation phase step with organic phase, which can be concerned as a model of cellular membrane. Two globular proteins lysozyme and human serum albumin tested. We have determined the conditions of tritium labeling when labeled by-products can be easy separated by means of dialysis and size-exclusion chromatography. Scintillation phase experiments were conducted for three types of organic liquids. Thus, the influences of the nature of organic phase on proteins adsorption and its distribution in the bulk of aqueous/organic liquid system were determined. It was found that proteins possess high surface activity at aqueous/organic liquid interface. Furthermore, values of hydrophobicity of globular proteins were found by the experiment. (author)

  7. Problem-Solving Test: The Mechanism of Protein Synthesis

    Science.gov (United States)

    Szeberenyi, Jozsef

    2009-01-01

    Terms to be familiar with before you start to solve the test: protein synthesis, ribosomes, amino acids, peptides, peptide bond, polypeptide chain, N- and C-terminus, hemoglobin, [alpha]- and [beta]-globin chains, radioactive labeling, [[to the third power]H] and [[to the fourteenth power]C]leucine, cytosol, differential centrifugation, density…

  8. Problem-Solving Test: Submitochondrial Localization of Proteins

    Science.gov (United States)

    Szeberenyi, Jozsef

    2011-01-01

    Mitochondria are surrounded by two membranes (outer and inner mitochondrial membrane) that separate two mitochondrial compartments (intermembrane space and matrix). Hundreds of proteins are distributed among these submitochondrial components. A simple biochemical/immunological procedure is described in this test to determine the localization of…

  9. Pregalactic formation of globular clusters in cold dark matter

    International Nuclear Information System (INIS)

    Faber, S.M.; Blumenthal, G.R.; Rosenblatt, E.I.

    1988-01-01

    The pregalactic hypothesis for the formation of globular clusters is reconsidered in the light of Zinn's (1985) discovery of a two-component globular population in the Milky Way. For a cold dark matter spectrum, high-sigma fluctuations of 10 to the 5th - 10 to the 6th solar masses are assumed to be the progenitors of the spheroidal population of globular clusters. The mass fraction of globular clusters in galaxies then requires that perturbations above roughly 2.8 sigma survive as globulars, and their observed radii require baryonic collapse factors of order 10. Such an absolute density threshold for globular cluster formation achieves adequate fits to observed cluster radii and densities, the mass fraction of globulars versus Hubble type, the radial density profile of globulars within galaxies, and the globular luminosity function. However, a fixed density threshold criterion for cluster survival lacks convincing physical justification and does not by itself explain the homogeneous metallicities within clusters or the large metallicity variations from cluster to cluster and from galaxy to galaxy. 33 references

  10. CVs and millisecond pulsar progenitors in globular clusters

    Science.gov (United States)

    Grindlay, J. E.; Cool, A. M.; Bailyn, C. D.

    1991-01-01

    The recent discovery of a large population of millisecond pulsars in globular clusters, together with earlier studies of both low luminosity X-ray sources and LMXBs in globulars, suggest there should be significant numbers of CVs in globulars. Although they have been searched for without success in selected cluster X-ray source fields, systematic surveys are lacking and would constrain binary production and both stellar and dynamical evolution in globular clusters. We describe the beginnings of such a search, using narrow band H-alpha imaging, and the sensitivities it might achieve.

  11. 21 CFR 862.1640 - Protein-bound iodine test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Protein-bound iodine test system. 862.1640 Section... Systems § 862.1640 Protein-bound iodine test system. (a) Identification. A protein-bound iodine test system is a device intended to measure protein-bound iodine in serum. Measurements of protein-bound...

  12. THE SIZE DIFFERENCE BETWEEN RED AND BLUE GLOBULAR CLUSTERS IS NOT DUE TO PROJECTION EFFECTS

    International Nuclear Information System (INIS)

    Webb, Jeremy J.; Harris, William E.; Sills, Alison

    2012-01-01

    Metal-rich (red) globular clusters in massive galaxies are, on average, smaller than metal-poor (blue) globular clusters. One of the possible explanations for this phenomenon is that the two populations of clusters have different spatial distributions. We test this idea by comparing clusters observed in unusually deep, high signal-to-noise images of M87 with a simulated globular cluster population in which the red and blue clusters have different spatial distributions, matching the observations. We compare the overall distribution of cluster effective radii as well as the relationship between effective radius and galactocentric distance for both the observed and simulated red and blue sub-populations. We find that the different spatial distributions does not produce a significant size difference between the red and blue sub-populations as a whole or at a given galactocentric distance. These results suggest that the size difference between red and blue globular clusters is likely due to differences during formation or later evolution.

  13. The Size Difference between Red and Blue Globular Clusters is not due to Projection Effects

    Science.gov (United States)

    Webb, Jeremy J.; Harris, William E.; Sills, Alison

    2012-11-01

    Metal-rich (red) globular clusters in massive galaxies are, on average, smaller than metal-poor (blue) globular clusters. One of the possible explanations for this phenomenon is that the two populations of clusters have different spatial distributions. We test this idea by comparing clusters observed in unusually deep, high signal-to-noise images of M87 with a simulated globular cluster population in which the red and blue clusters have different spatial distributions, matching the observations. We compare the overall distribution of cluster effective radii as well as the relationship between effective radius and galactocentric distance for both the observed and simulated red and blue sub-populations. We find that the different spatial distributions does not produce a significant size difference between the red and blue sub-populations as a whole or at a given galactocentric distance. These results suggest that the size difference between red and blue globular clusters is likely due to differences during formation or later evolution.

  14. HUBBLE PINPOINTS WHITE DWARFS IN GLOBULAR CLUSTER

    Science.gov (United States)

    2002-01-01

    Peering deep inside a cluster of several hundred thousand stars, NASA's Hubble Space Telescope uncovered the oldest burned-out stars in our Milky Way Galaxy. Located in the globular cluster M4, these small, dying stars - called white dwarfs - are giving astronomers a fresh reading on one of the biggest questions in astronomy: How old is the universe? The ancient white dwarfs in M4 are about 12 to 13 billion years old. After accounting for the time it took the cluster to form after the big bang, astronomers found that the age of the white dwarfs agrees with previous estimates for the universe's age. In the top panel, a ground-based observatory snapped a panoramic view of the entire cluster, which contains several hundred thousand stars within a volume of 10 to 30 light-years across. The Kitt Peak National Observatory's 0.9-meter telescope took this picture in March 1995. The box at left indicates the region observed by the Hubble telescope. The Hubble telescope studied a small region of the cluster. A section of that region is seen in the picture at bottom left. A sampling of an even smaller region is shown at bottom right. This region is only about one light-year across. In this smaller region, Hubble pinpointed a number of faint white dwarfs. The blue circles pinpoint the dwarfs. It took nearly eight days of exposure time over a 67-day period to find these extremely faint stars. Globular clusters are among the oldest clusters of stars in the universe. The faintest and coolest white dwarfs within globular clusters can yield a globular cluster's age. Earlier Hubble observations showed that the first stars formed less than 1 billion years after the universe's birth in the big bang. So, finding the oldest stars puts astronomers within arm's reach of the universe's age. M4 is 7,000 light-years away in the constellation Scorpius. Hubble's Wide Field and Planetary Camera 2 made the observations from January through April 2001. These optical observations were combined to

  15. Synthetic properties of models of globular clusters

    Energy Technology Data Exchange (ETDEWEB)

    Angeletti, L; Dolcetta, R; Giannone, P. (Rome Univ. (Italy). Osservatorio Astronomico)

    1980-05-01

    Synthetic and projected properties of models of globular clusters have been computed on the basis of stellar evolution and time changes of the dynamical cluster structure. Clusters with five and eight stellar groups (each group consisting of stars with the same mass) were studied. Mass loss from evolved stars was taken into account. Observational features were obtained at ages of 10-19 x 10/sup 9/ yr. The basic importance of the horizontal- and asymptotic-branch stars was pointed out. A comparison of the results with observed data of M3 is discussed with the purpose of obtaining general indications rather than a specific fit.

  16. Synthetic properties of models of globular clusters

    International Nuclear Information System (INIS)

    Angeletti, L.; Dolcetta, R.; Giannone, P.

    1980-01-01

    Synthetic and projected properties of models of globular clusters have been computed on the basis of stellar evolution and time changes of the dynamical cluster structure. Clusters with five and eight stellar groups (each group consisting of stars with the same mass) were studied. Mass loss from evolved stars was taken into account. Observational features were obtained at ages of 10-19 x 10 9 yr. The basic importance of the horizontal- and asymptotic-branch stars was pointed out. A comparison of the results with observed data of M3 is discussed with the purpose of obtaining general indications rather than a specific fit. (orig.)

  17. Deep radio synthesis images of globular clusters

    International Nuclear Information System (INIS)

    Kulkarni, S.R.; Goss, W.M.; Wolszczan, A.; Middleditch, J.

    1990-01-01

    Results are reported from a program of high-resolution and high-sensitivity imaging of globular clusters at 20 cm. The findings indicate that there is not a large number of pulsars in compact binaries which have escaped detection in single-dish pulse searches. Such binaries have been postulated to result from tidal captures of single main-sequence stars. It is suggested that most tidal captures involving neutron stars ultimately result in the formation of a spun-up single pulsar and the complete disruption of the main-sequence star. 27 refs

  18. Near infrared photometry of globular clusters

    International Nuclear Information System (INIS)

    Evans, T.L.; Menzies, J.W.

    1977-01-01

    Photographic photometry on the V, Isub(K) system has been obtained for giant stars in the metal-rich globular clusters NGC 5927, 6171, 6352, 6356, 6388, 6522, 6528, 6712 and 6723. Colour-magnitude diagrams are presented. These data, with earlier observations of NGC 104 (47 Tuc), yield new parameters to describe the giant branch. These are the colour of the red variables, represented by their mean colour (V - Isub(K)) 0 or by the colour (V - Isub(K))sub(BO) of the bluest red variable on the giant branch of a cluster, and ΔV' which is the magnitude difference between the horizontal branch and the highest point on the giant branch. The latter is independent of reddening, since the giant branch of the most metal-rich clusters passes through a maximum in the V, V - Isub(K) plane. These parameters are correlated with the metal content, deduced from integrated photometry: the red variables are redder and the giant branch fainter the higher the metal content. Comparison with theoretical evolutionary tracks suggests that the range in metal content of these clusters is at most a factor of 10, the most metal-rich clusters possibly approaching the solar value. The cluster giant branches and those of open clusters, groups and field stars of the old disk population are compared. The assumption that all the globular clusters have an absolute magnitude on the horizontal branch of Msub(v) = + 0.9, as found recently for 47 Tuc, gives good agreement between the magnitudes of giant stars in the most metal rich of the globular clusters and those of field stars deduced from statistical parallaxes and moving group parallaxes. The values of the parameters ΔV' and (V - Isub(k))sub(BO) also approach those in the moving groups. The globular clusters have a longer horizontal branch, however, and the subgiants are bluer even when the values of ) 7Fe/H{ appear to be the same. (author). )

  19. Tuberculosis skin testing, anergy and protein malnutrition in Peru.

    Science.gov (United States)

    Pelly, T F; Santillan, C F; Gilman, R H; Cabrera, L Z; Garcia, E; Vidal, C; Zimic, M J; Moore, D A J; Evans, C A

    2005-09-01

    Malnutrition and intestinal parasites cause immunosuppression. This may cause false-negative tuberculin skin tests (TST) and failure to identify tuberculosis (TB) infection. To assess factors associated with TST positivity and anergy in disadvantaged communities in Peru. A study of 212 randomly selected adults: 102 in a rural Amazonian village and 110 shanty town residents in urban Lima. Respectively 52% and 53% of urban and rural jungle populations were TST-positive. Using simultaneous tetanus and candida skin tests, 99% had at least one positive skin test. Generalised anergy was therefore rare, despite frequent intestinal parasitic infection, including 34% helminth infection prevalence in the jungle. TST positivity was associated with age (P = 0.001), known TB contact (P = 0.02) and poor household ventilation (P = 0.007). TST positivity was not significantly associated with crowding, reported past TB, single/multiple BCG vaccination, income, intestinal parasites, dietary factors, body mass index or body fat. Individuals with lower anthropometric body protein, as measured by corrected arm muscle area, were less likely to be TST-positive (P = 0.02), implying that protein malnutrition caused tuberculin-specific anergy. These results identify the importance of household ventilation for community TB transmission and add to the evidence that protein malnutrition suppresses TB immunity, causing false-negative TST results.

  20. THE PRODUCTION RATE OF SN Ia EVENTS IN GLOBULAR CLUSTERS

    International Nuclear Information System (INIS)

    Washabaugh, Pearce C.; Bregman, Joel N.

    2013-01-01

    In globular clusters, dynamical evolution produces luminous X-ray emitting binaries at a rate about 200 times greater than in the field. If globular clusters also produce SN Ia at a high rate, it would account for many of the SN Ia production in early-type galaxies and provide insight into their formation. Here we use archival Hubble Space Telescope (HST) images of nearby galaxies that have hosted an SN Ia to examine the rate at which globular clusters produce these events. The location of the SN Ia is registered on an HST image obtained before the event or after the supernova (SN) faded. Of the 36 nearby galaxies examined, 21 had sufficiently good data to search for globular cluster hosts. None of the 21 SNe have a definite globular cluster counterpart, although there are some ambiguous cases. This places an upper limit to the enhancement rate of SN Ia production in globular clusters of about 42 at the 95% confidence level, which is an order of magnitude lower than the enhancement rate for luminous X-ray binaries. Even if all of the ambiguous cases are considered as having a globular cluster counterpart, the upper bound for the enhancement rate is 82 at the 95% confidence level, still a factor of several below that needed to account for half of the SN Ia events. Barring unforeseen selection effects, we conclude that globular clusters are not responsible for producing a significant fraction of the SN Ia events in early-type galaxies.

  1. Analytical Solution for Stellar Density in Globular Clusters MA Sharaf

    Indian Academy of Sciences (India)

    Introduction. A globular cluster is a spherical collection of stars that orbits a galactic core as a satellite. They are generally composed of hundreds of thousands of low-metal, old stars. The types of stars found in a globular cluster are similar to those in the bulge of a spiral galaxy but confined to a volume of only a few cubic ...

  2. NONLINEAR COLOR-METALLICITY RELATIONS OF GLOBULAR CLUSTERS. II. A TEST ON THE NONLINEARITY SCENARIO FOR COLOR BIMODALITY USING THE u-BAND COLORS: THE CASE OF M87 (NGC 4486)

    International Nuclear Information System (INIS)

    Yoon, Suk-Jin; Lee, Sang-Yoon; Kim, Hak-Sub; Cho, Jaeil; Chung, Chul; Sohn, Sangmo T.; Blakeslee, John P.

    2011-01-01

    The optical color distributions of globular clusters (GCs) in most large elliptical galaxies are bimodal. Based on the assumed linear relationship between GC colors and their metallicities, the bimodality has been taken as evidence of two GC subsystems with different metallicities in each galaxy and has led to a number of theories in the context of galaxy formation. More recent observations and modeling of GCs, however, suggests that the color-metallicity relations (CMRs) are inflected, and thus colors likely trace metallicities in a nonlinear manner. The nonlinearity could produce bimodal color distributions from a broad underlying metallicity spread, even if it is unimodal. Despite the far-reaching implications, whether CMRs are nonlinear and whether the nonlinearity indeed causes the color bimodality are still open questions. Given that the spectroscopic refinement of CMRs is still very challenging, we here propose a new photometric technique to probe the possible nonlinear nature of CMRs. In essence, a color distribution of GCs is a 'projected' distribution of their metallicities. Since the form of CMRs hinges on which color is used, the shape of color distributions varies depending significantly on the colors. Among other optical colors, the u-band related colors (e.g., u – g and u – z) are theoretically predicted to exhibit significantly less inflected CMRs than other preferred CMRs (e.g., for g – z). As a case study, we performed the Hubble Space Telescope (HST)/WFPC2 archival u-band photometry for the M87 (NGC 4486) GC system with confirmed color bimodality. We show that the u-band color distributions are significantly different from that of g – z and consistent with our model predictions. With more u-band measurements, this method will support or rule out the nonlinear CMR scenario for the origin of GC color bimodality with high confidence. The HST/WFC3 observations in F336W for nearby large elliptical galaxies are highly anticipated in this regard.

  3. The Frequency of Binary Stars in the Globular Cluster M71

    Science.gov (United States)

    Barden, S. C.; Armandroff, T. E.; Pryor, C. P.

    1994-12-01

    The frequency of binary stars is a fundamental property of a stellar population. A comparison of the frequency of binaries in globular clusters with those in the field halo and disk populations tests the similarity of star formation in those environments. Binary stars in globular clusters also act as an energy source which ``heats" the cluster through super-elastic encounters with other stars and binaries. Such encounters can not only profoundly affect the dynamical evolution of the cluster, they can disrupt the widely separated binaries and catalyze the formation of exotic objects such as blue stragglers, x-ray binaries, and milli-second pulsars. We have used the KPNO 4-m and the multi-fiber instruments Nessie and Hydra to measure radial velocities at 4 epochs over two years for a sample of 126 stars in the globular cluster M71. Velocity errors are under 1 km s(-1) for the brighter stars and under 2 km s(-1) for the majority of our data set. These velocities will be valuable for studying the kinematics of M71, but here we focus on searching for binaries. The faintest stars are at V=17, or just above the main sequence turnoff. Our sample is thus deeper than any published globular cluster binary search utilizing spectroscopic techniques. By observing smaller stars, we double the number of decades of binary periods sampled compared to previous studies and come within a factor of 4 of the shortest possible periods for turnoff stars. This wider period window has produced the largest known sample of binaries in a globular cluster. Four stars show velocity ranges larger than 20 km s(-1) , nine have ranges larger than 10 km s(-1) , and others are clearly variable. We will compare the radial distribution of these stars to that predicted by theory and derive the main-sequence binary fraction.

  4. Course 12: Proteins: Structural, Thermodynamic and Kinetic Aspects

    Science.gov (United States)

    Finkelstein, A. V.

    1 Introduction 2 Overview of protein architectures and discussion of physical background of their natural selection 2.1 Protein structures 2.2 Physical selection of protein structures 3 Thermodynamic aspects of protein folding 3.1 Reversible denaturation of protein structures 3.2 What do denatured proteins look like? 3.3 Why denaturation of a globular protein is the first-order phase transition 3.4 "Gap" in energy spectrum: The main characteristic that distinguishes protein chains from random polymers 4 Kinetic aspects of protein folding 4.1 Protein folding in vivo 4.2 Protein folding in vitro (in the test-tube) 4.3 Theory of protein folding rates and solution of the Levinthal paradox

  5. Conformational energy calculations on polypeptides and proteins: use of a statistical mechanical procedure for evaluating structure and properties.

    Science.gov (United States)

    Scheraga, H A; Paine, G H

    1986-01-01

    We are using a variety of theoretical and computational techniques to study protein structure, protein folding, and higher-order structures. Our earlier work involved treatments of liquid water and aqueous solutions of nonpolar and polar solutes, computations of the stabilities of the fundamental structures of proteins and their packing arrangements, conformations of small cyclic and open-chain peptides, structures of fibrous proteins (collagen), structures of homologous globular proteins, introduction of special procedures as constraints during energy minimization of globular proteins, and structures of enzyme-substrate complexes. Recently, we presented a new methodology for predicting polypeptide structure (described here); the method is based on the calculation of the probable and average conformation of a polypeptide chain by the application of equilibrium statistical mechanics in conjunction with an adaptive, importance sampling Monte Carlo algorithm. As a test, it was applied to Met-enkephalin.

  6. Edades relativas de cúmulos globulares

    Science.gov (United States)

    Miller Bertolami, M.; Forte, J. C.

    El trabajo de Rossemberg et al (1999), estudia las edades relativas de cúmulos globulares galácticos mediante el análisis de ciertos parámetros morfológicos de los diagramas color-magnitud de dichos cúmulos. Este trabajo se centra en tres puntos: analizar la consistencia de los resultados obtenidos por Rossemberg et al (1999) al emplear observaciones en el sistema fotométrico de Washington, más precisamente, las magnitudes C y T1 en lugar de las magnitudes V e I utilizadas por dichos autores. De la existencia de colores integrados, metalicidad y edad (relativa) para 21 de los cúmulos utilizados en dicho trabajo, se analiza la consistencia de estos resultados con las dependencias de color integrado como función de la edad y la metalicidad que se desprenden de los modelos teóricos de luz integrada por Worthey (1994), Schulz (2002) y Lee et al (2002). Por último se lleva a cabo una breve comparación de la morfología de los diagramas color-magnitud de los cúmulos globulares y de las isocronas utilizadas, a fin de intentar identificar algunas de las posibles causas de las diferencias observadas en los incisos anteriores.

  7. LISA Sources in Milky Way Globular Clusters

    Science.gov (United States)

    Kremer, Kyle; Chatterjee, Sourav; Breivik, Katelyn; Rodriguez, Carl L.; Larson, Shane L.; Rasio, Frederic A.

    2018-05-01

    We explore the formation of double-compact-object binaries in Milky Way (MW) globular clusters (GCs) that may be detectable by the Laser Interferometer Space Antenna (LISA). We use a set of 137 fully evolved GC models that, overall, effectively match the properties of the observed GCs in the MW. We estimate that, in total, the MW GCs contain ˜21 sources that will be detectable by LISA. These detectable sources contain all combinations of black hole (BH), neutron star, and white dwarf components. We predict ˜7 of these sources will be BH-BH binaries. Furthermore, we show that some of these BH-BH binaries can have signal-to-noise ratios large enough to be detectable at the distance of the Andromeda galaxy or even the Virgo cluster.

  8. Constraints on H(0) from globular clusters

    International Nuclear Information System (INIS)

    Vandenberg, D.A.

    1988-01-01

    On the basis of canonical stellar evolutionary computations, the maximum age of the globular clusters is suggested to be near either 14 Gyr or 18 Gyr, depending on how (O/Fe) varies with (Fe/H) in the cluster stars. The lower estimate requires that H(0) = 65 km/s/Mpc or less, for all Omega(0) = O or greater, if the standard Big-Bang cosmological theory is correct - while the higher age value similarly constrains the Hubble constant to be smaller than 46 km/s/Mpc. Some reduction in the upper limit to cluster ages and a consequent increase in H(0) may be expected if helium diffusion is important in Population II stars; nevertheless, values of H(0) greater than 75 km/s/Mpc still appear to be precluded unless the cosmological constant is nonzero. 51 refs

  9. Extragalactic globular clusters. I. The metallicity calibration

    International Nuclear Information System (INIS)

    Brodie, J.P.; Huchra, J.P.

    1990-01-01

    The ability of absorption-line strength indices, measured from integrated globular cluster spectra, to predict mean cluster metallicity is explored. Statistical criteria, are used to identify the six best indices out of about 20 measured in a large sample of Galactic and M31 cluster spectra. Linear relations between index and metallicity have been derived along with new calibrations of infrared colors (V - K, J - K, and CO) versus Fe/H. Estimates of metallicity from the six spectroscopic index-metallicity relations have been combined in three different ways to identify the most efficient estimator and the minimum bias estimator of Fe/H - the weighted mean. This provides an estimate of Fe/H accurate to about 15 percent. 37 refs

  10. Individual globular domains and domain unfolding visualized in overstretched titin molecules with atomic force microscopy.

    Directory of Open Access Journals (Sweden)

    Zsolt Mártonfalvi

    Full Text Available Titin is a giant elastomeric protein responsible for the generation of passive muscle force. Mechanical force unfolds titin's globular domains, but the exact structure of the overstretched titin molecule is not known. Here we analyzed, by using high-resolution atomic force microscopy, the structure of titin molecules overstretched with receding meniscus. The axial contour of the molecules was interrupted by topographical gaps with a mean width of 27.7 nm that corresponds well to the length of an unfolded globular (immunoglobulin and fibronectin domain. The wide gap-width distribution suggests, however, that additional mechanisms such as partial domain unfolding and the unfolding of neighboring domain multimers may also be present. In the folded regions we resolved globules with an average spacing of 5.9 nm, which is consistent with a titin chain composed globular domains with extended interdomain linker regions. Topographical analysis allowed us to allocate the most distal unfolded titin region to the kinase domain, suggesting that this domain systematically unfolds when the molecule is exposed to overstretching forces. The observations support the prediction that upon the action of stretching forces the N-terminal ß-sheet of the titin kinase unfolds, thus exposing the enzyme's ATP-binding site and hence contributing to the molecule's mechanosensory function.

  11. LITHIUM-RICH GIANTS IN GLOBULAR CLUSTERS

    Energy Technology Data Exchange (ETDEWEB)

    Kirby, Evan N.; Cohen, Judith G. [California Institute of Technology, 1200 E. California Boulevard, MC 249-17, Pasadena, CA 91125 (United States); Guhathakurta, Puragra [UCO/Lick Observatory and Department of Astronomy and Astrophysics, University of California, 1156 High Street, Santa Cruz, CA 95064 (United States); Zhang, Andrew J. [The Harker School, 500 Saratoga Avenue, San Jose, CA 95129 (United States); Hong, Jerry [Palo Alto High School, 50 Embarcadero Road, Palo Alto, CA, 94301 (United States); Guo, Michelle [Stanford University, 450 Serra Mall, Stanford, CA 94305 (United States); Guo, Rachel [Irvington High School, 41800 Blacow Road, Fremont, CA 94538 (United States); Cunha, Katia [Observatório Nacional, São Cristóvão Rio de Janeiro (Brazil)

    2016-03-10

    Although red giants deplete lithium on their surfaces, some giants are Li-rich. Intermediate-mass asymptotic giant branch (AGB) stars can generate Li through the Cameron–Fowler conveyor, but the existence of Li-rich, low-mass red giant branch (RGB) stars is puzzling. Globular clusters are the best sites to examine this phenomenon because it is straightforward to determine membership in the cluster and to identify the evolutionary state of each star. In 72 hours of Keck/DEIMOS exposures in 25 clusters, we found four Li-rich RGB and two Li-rich AGB stars. There were 1696 RGB and 125 AGB stars with measurements or upper limits consistent with normal abundances of Li. Hence, the frequency of Li-richness in globular clusters is (0.2 ± 0.1)% for the RGB, (1.6 ± 1.1)% for the AGB, and (0.3 ± 0.1)% for all giants. Because the Li-rich RGB stars are on the lower RGB, Li self-generation mechanisms proposed to occur at the luminosity function bump or He core flash cannot explain these four lower RGB stars. We propose the following origin for Li enrichment: (1) All luminous giants experience a brief phase of Li enrichment at the He core flash. (2) All post-RGB stars with binary companions on the lower RGB will engage in mass transfer. This scenario predicts that 0.1% of lower RGB stars will appear Li-rich due to mass transfer from a recently Li-enhanced companion. This frequency is at the lower end of our confidence interval.

  12. Most Massive Globular Cluster in Our Galaxy

    Science.gov (United States)

    1994-05-01

    Far down in the southern sky, in the constellation of Centaurus, a diffuse spot of light can be perceived with the unaided eye. It may be unimpressive, but when seen through a telescope, it turns out to be a beautiful, dense cluster of innumerable stars [1]. Omega Centauri, as this object is called, is the brightest of its type in the sky. We refer to it as a "globular cluster", due to its symmetric form. It belongs to our Milky Way galaxy and astrophysical investigations have shown that it is located at a distance of about 16,500 light-years (1 light-year = 9,460,000,000,000 km). Nobody knows for sure how many individual stars it contains, but recent estimates run into the millions. Most of these stars are more than 10,000 million years old and it is generally agreed that Omega Centauri has a similar age. Measurements of its motion indicate that Omega Centauri plows through the Milky Way in an elongated orbit. It is not easy to understand how it has managed to keep its stars together during such an extended period. MEASURING STELLAR VELOCITIES IN OMEGA CENTAURI A group of astronomers [2] have recently carried through a major investigation of Omega Centauri. After many nights of observations at the ESO La Silla observatory, they now conclude that not only is this globular cluster the brightest, it is indeed by far the most massive known in the Milky Way. The very time-consuming observations were made during numerous observing sessions over a period of no less than 13 years (1981-1993), with the photoelectric spectrometer CORAVEL mounted on the 1.5-m Danish telescope at La Silla. The CORAVEL instrument (COrelation RAdial VELocities) was built in a joint effort between the Geneva (Switzerland) and Marseilles (France) observatories. It functions according to the cross-correlation technique, by means of which the spectrum of the observed star is compared with a "standard stellar spectrum" [3]. HOW HEAVY IS OMEGA CENTAURI? In the present study, a total of 1701

  13. Observations of CO and OI in stars in globular clusters

    International Nuclear Information System (INIS)

    Wallerstein, G.; Pilachowski, C.

    1978-01-01

    Since studies at classification dispersion and early analyses of high dispersion spectra have yielded little quantitative data on the abundances of C, N, and O in globular clusters the authors have been endeavoring to establish their abundances in stars in several clusters. The problem has been approached in two ways, by observing the 2.3 micron CO bands and the 6300 A [OI] line in individual stars in globular clusters. (Auth.)

  14. GLOBULAR CLUSTERS AS CRADLES OF LIFE AND ADVANCED CIVILIZATIONS

    Energy Technology Data Exchange (ETDEWEB)

    Stefano, R. Di [Harvard-Smithsonian Center for Astrophysics (United States); Ray, A., E-mail: rdistefano@cfa.harvard.edu, E-mail: akr@tifr.res.in [Tata Institute of Fundamental Research (India)

    2016-08-10

    Globular clusters are ancient stellar populations in compact dense ellipsoids. There is no star formation and there are no core-collapse supernovae, but several lines of evidence suggest that globular clusters are rich in planets. If so, and if advanced civilizations can develop there, then the distances between these civilizations and other stars would be far smaller than typical distances between stars in the Galactic disk, facilitating interstellar communication and travel. The potent combination of long-term stability and high stellar densities provides a globular cluster opportunity. Yet the very proximity that promotes interstellar travel also brings danger, as stellar interactions can destroy planetary systems. We find, however, that large portions of many globular clusters are “sweet spots,” where habitable-zone planetary orbits are stable for long times. Globular clusters in our own and other galaxies are, therefore, among the best targets for searches for extraterrestrial intelligence (SETI). We use the Drake equation to compare the likelihood of advanced civilizations in globular clusters to that in the Galactic disk. We also consider free-floating planets, since wide-orbit planets can be ejected to travel through the cluster. Civilizations spawned in globular clusters may be able to establish self-sustaining outposts, reducing the probability that a single catastrophic event will destroy the civilization. Although individual civilizations may follow different evolutionary paths, or even be destroyed, the cluster may continue to host advanced civilizations once a small number have jumped across interstellar space. Civilizations residing in globular clusters could therefore, in a sense, be immortal.

  15. Effect of radiation pressure in the cores of globular clusters

    Energy Technology Data Exchange (ETDEWEB)

    Angeletti, L; Capuzzo-Dolcetta, R; Giannone

    1981-10-01

    The possible effects of a presence of a dust cloud in the cores of globular clusters was investigated. Two cluster models were considered together with various models of clouds. The problem of radiation transfer was solved under some simplifying assumptions. Owing to a differential absorption of the star light in the cloud, radiation pressure turned out be inward-directed in some cloud models. This fact may lead to a confinement of some dust in the central regions of globular clusters.

  16. Globular cluster metallicity scale: evidence from stellar models

    International Nuclear Information System (INIS)

    Demarque, P.; King, C.R.; Diaz, A.

    1982-01-01

    Theoretical giant branches have been constructed to determine their relative positions for metallicities in the range -2.3 0 )/sub 0,g/ based on these models is presented which yields good agreement over the observed range of metallicities for galactic globular clusters and old disk clusters. The metallicity of 47 Tuc and M71 given by this calibration is about -0.8 dex. Subject headings: clusters, globular: stars: abundances: stars: interiors

  17. GLOBULAR CLUSTERS AS CRADLES OF LIFE AND ADVANCED CIVILIZATIONS

    International Nuclear Information System (INIS)

    Stefano, R. Di; Ray, A.

    2016-01-01

    Globular clusters are ancient stellar populations in compact dense ellipsoids. There is no star formation and there are no core-collapse supernovae, but several lines of evidence suggest that globular clusters are rich in planets. If so, and if advanced civilizations can develop there, then the distances between these civilizations and other stars would be far smaller than typical distances between stars in the Galactic disk, facilitating interstellar communication and travel. The potent combination of long-term stability and high stellar densities provides a globular cluster opportunity. Yet the very proximity that promotes interstellar travel also brings danger, as stellar interactions can destroy planetary systems. We find, however, that large portions of many globular clusters are “sweet spots,” where habitable-zone planetary orbits are stable for long times. Globular clusters in our own and other galaxies are, therefore, among the best targets for searches for extraterrestrial intelligence (SETI). We use the Drake equation to compare the likelihood of advanced civilizations in globular clusters to that in the Galactic disk. We also consider free-floating planets, since wide-orbit planets can be ejected to travel through the cluster. Civilizations spawned in globular clusters may be able to establish self-sustaining outposts, reducing the probability that a single catastrophic event will destroy the civilization. Although individual civilizations may follow different evolutionary paths, or even be destroyed, the cluster may continue to host advanced civilizations once a small number have jumped across interstellar space. Civilizations residing in globular clusters could therefore, in a sense, be immortal.

  18. The nature of the globular- to fibrous-actin transition.

    Science.gov (United States)

    Oda, Toshiro; Iwasa, Mitsusada; Aihara, Tomoki; Maéda, Yuichiro; Narita, Akihiro

    2009-01-22

    Actin plays crucial parts in cell motility through a dynamic process driven by polymerization and depolymerization, that is, the globular (G) to fibrous (F) actin transition. Although our knowledge about the actin-based cellular functions and the molecules that regulate the G- to F-actin transition is growing, the structural aspects of the transition remain enigmatic. We created a model of F-actin using X-ray fibre diffraction intensities obtained from well oriented sols of rabbit skeletal muscle F-actin to 3.3 A in the radial direction and 5.6 A along the equator. Here we show that the G- to F-actin conformational transition is a simple relative rotation of the two major domains by about 20 degrees. As a result of the domain rotation, the actin molecule in the filament is flat. The flat form is essential for the formation of stable, helical F-actin. Our F-actin structure model provides the basis for understanding actin polymerization as well as its molecular interactions with actin-binding proteins.

  19. Metallicity Spreads in M31 Globular Clusters

    Science.gov (United States)

    Bridges, Terry

    2003-07-01

    Our recent deep HST photometry of the M31 halo globular cluster {GC} Mayall II, also called G1, has revealed a red-giant branch with a clear spread that we attribute to an intrinsic metallicity dispersion of at least 0.4 dex in [Fe/H]. The only other GC exhibiting such a metallicity dispersion is Omega Centauri, the brightest and most massive Galactic GC, whose range in [Fe/H] is about 0.5 dex. These observations are obviously linked to the fact that both G1 and Omega Cen are bright and massive GC, with potential wells deep enough to keep part of their gas, which might have been recycled, producing a metallicity scatter among cluster stars. These observations dramatically challenge the notion of chemical homogeneity as a defining characteristic of GCs. It is critically important to find out how common this phenomenon is and how it can constrain scenarios/models of GC formation. The obvious targets are other bright and massive GCs, which exist in M31 but not in our Galaxy where Omega Cen is an isolated giant. We propose to acquire, with ACS/HRC, deep imaging of 3 of the brightest M31 GCs for which we have observed velocity dispersion values similar to those observed in G1 and Omega Cen. A sample of GCs with chemical abundance dispersions will provide essential information about their formation mechanism. This would represent a major step for the studies of the origin and evolution of stellar populations.

  20. CENTRAL ROTATIONS OF MILKY WAY GLOBULAR CLUSTERS

    International Nuclear Information System (INIS)

    Fabricius, Maximilian H.; Rukdee, Surangkhana; Saglia, Roberto P.; Bender, Ralf; Hopp, Ulrich; Thomas, Jens; Williams, Michael J.; Noyola, Eva; Opitsch, Michael

    2014-01-01

    Most Milky Way globular clusters (GCs) exhibit measurable flattening, even if on a very low level. Both cluster rotation and tidal fields are thought to cause this flattening. Nevertheless, rotation has only been confirmed in a handful of GCs, based mostly on individual radial velocities at large radii. We are conducting a survey of the central kinematics of Galactic GCs using the new Integral Field Unit instrument VIRUS-W. We detect rotation in all 11 GCs that we have observed so far, rendering it likely that a large majority of the Milky Way GCs rotate. We use published catalogs of GCs to derive central ellipticities and position angles. We show that in all cases where the central ellipticity permits an accurate measurement of the position angle, those angles are in excellent agreement with the kinematic position angles that we derive from the VIRUS-W velocity fields. We find an unexpected tight correlation between central rotation and outer ellipticity, indicating that rotation drives flattening for the objects in our sample. We also find a tight correlation between central rotation and published values for the central velocity dispersion, most likely due to rotation impacting the old dispersion measurements

  1. Central Rotations of Milky Way Globular Clusters

    Science.gov (United States)

    Fabricius, Maximilian H.; Noyola, Eva; Rukdee, Surangkhana; Saglia, Roberto P.; Bender, Ralf; Hopp, Ulrich; Thomas, Jens; Opitsch, Michael; Williams, Michael J.

    2014-06-01

    Most Milky Way globular clusters (GCs) exhibit measurable flattening, even if on a very low level. Both cluster rotation and tidal fields are thought to cause this flattening. Nevertheless, rotation has only been confirmed in a handful of GCs, based mostly on individual radial velocities at large radii. We are conducting a survey of the central kinematics of Galactic GCs using the new Integral Field Unit instrument VIRUS-W. We detect rotation in all 11 GCs that we have observed so far, rendering it likely that a large majority of the Milky Way GCs rotate. We use published catalogs of GCs to derive central ellipticities and position angles. We show that in all cases where the central ellipticity permits an accurate measurement of the position angle, those angles are in excellent agreement with the kinematic position angles that we derive from the VIRUS-W velocity fields. We find an unexpected tight correlation between central rotation and outer ellipticity, indicating that rotation drives flattening for the objects in our sample. We also find a tight correlation between central rotation and published values for the central velocity dispersion, most likely due to rotation impacting the old dispersion measurements. This Letter includes data taken at The McDonald Observatory of The University of Texas at Austin.

  2. Endotoxin testing of proteins for parenteral administration using the Mono Mac 6 assay

    DEFF Research Database (Denmark)

    Moesby, Lise; Hansen, E W; Christensen, J D

    2000-01-01

    Pharmaceutical products containing proteins cause problems in testing for endotoxin and pyrogens. Many proteins interfere with the LAL test and the proteins are immunogenic in rabbits. The monocytic cell line Mono Mac 6 is an alternative assay for detection of endotoxin and other pyrogens....

  3. HORIZONTAL BRANCH MORPHOLOGY OF GLOBULAR CLUSTERS: A MULTIVARIATE STATISTICAL ANALYSIS

    International Nuclear Information System (INIS)

    Jogesh Babu, G.; Chattopadhyay, Tanuka; Chattopadhyay, Asis Kumar; Mondal, Saptarshi

    2009-01-01

    The proper interpretation of horizontal branch (HB) morphology is crucial to the understanding of the formation history of stellar populations. In the present study a multivariate analysis is used (principal component analysis) for the selection of appropriate HB morphology parameter, which, in our case, is the logarithm of effective temperature extent of the HB (log T effHB ). Then this parameter is expressed in terms of the most significant observed independent parameters of Galactic globular clusters (GGCs) separately for coherent groups, obtained in a previous work, through a stepwise multiple regression technique. It is found that, metallicity ([Fe/H]), central surface brightness (μ v ), and core radius (r c ) are the significant parameters to explain most of the variations in HB morphology (multiple R 2 ∼ 0.86) for GGC elonging to the bulge/disk while metallicity ([Fe/H]) and absolute magnitude (M v ) are responsible for GGC belonging to the inner halo (multiple R 2 ∼ 0.52). The robustness is tested by taking 1000 bootstrap samples. A cluster analysis is performed for the red giant branch (RGB) stars of the GGC belonging to Galactic inner halo (Cluster 2). A multi-episodic star formation is preferred for RGB stars of GGC belonging to this group. It supports the asymptotic giant branch (AGB) model in three episodes instead of two as suggested by Carretta et al. for halo GGC while AGB model is suggested to be revisited for bulge/disk GGC.

  4. Nanoparticle-protein corona in invertebrate in vitro testing

    DEFF Research Database (Denmark)

    Hayashi, Yuya; Miclaus, Teodora; Scavenius, Carsten

    2013-01-01

    , and the primary cells were thus exposed to silver nanoparticles with pre-formed corona of serum albumin (a major serum protein). Here we have profiled proteins forming the hard corona around silver nanoparticles (OECD reference materials, 15 nm and 75 nm) using gel electrophoresis techniques to identify proteins...... that strongly interact with the nanoparticles. This study was accompanied by multi-parametric flow-cytometry analysis of the cellular responses, in particular nanoparticle accumulation and cytotoxicity. The formation of and differential cellular responses to nanoparticle-protein complexes underscore the need...

  5. Globular Clusters Shine in a Galaxy Lacking Dark Matter

    Science.gov (United States)

    Kohler, Susanna

    2018-04-01

    You may have seen recent news about NGC 1052DF2, a galaxy that was discovered to have little or no dark matter. Now, a new study explores what NGC 1052DF2 does have: an enigmatic population of unusually large and luminous globular clusters.Keck/LRIS spectra (left and right) and HST images (center) of the 11 clusters associated with NGC 1052DF2. The color images each span 1 1. [van Dokkum et al. 2018]An Unusual DwarfThe ultra-diffuse galaxy NGC 1052DF2, originally identified with the Dragonfly Telescope Array, has puzzled astronomers since the discovery that its dynamical mass determined by the motions of globular-cluster-like objects spotted within it is essentially the same as its stellar mass. This equivalence implies that the galaxy is strangely lacking dark matter; the upper limit set on its dark matter halo is 400 times smaller than what we would expect for such a dwarf galaxy.Led by Pieter van Dokkum (Yale University), the team that made this discovery has now followed up with detailed Hubble Space Telescope imaging and Keck spectroscopy. Their goal? To explore the objects that allowed them to make the dynamical-mass measurement: the oddly bright globular clusters of NGC 1052DF2.Sizes (circularized half-light radii) vs. absolute magnitudes for globular clusters in NGC1052DF2 (black) and the Milky Way (red). [Adapted from van Dokkum et al. 2018]Whats Up with the Globular Clusters?Van Dokkum and collaborators spectroscopically confirmed 11 compact objects associated with the faint galaxy. These objects are globular-cluster-like in their appearance, but the peak of their luminosity distribution is offset by a factor of four from globular clusters of other galaxies; these globular clusters are significantly brighter than is typical.Using the Hubble imaging, the authors determined that NGC 1052DF2s globular clusters are more than twice the size of the Milky Ways globular clusters in the same luminosity range. As is typical for globular clusters, they are an old

  6. Photometric studies of globular clusters in the Andromeda nebula

    International Nuclear Information System (INIS)

    Sharov, A.S.; Lyutyj, V.M.

    1983-01-01

    The comparison of the frequency distribution of Bergh Q and Racine R metallicity parameters for globular clusters in the Galaxy and M31 is given. Mean values of the parameters are: in the Galaxy anti Q=-0.31 and anti R=0.40, in M31 anti Q=-0.32 and anti R=0.42. Hence the mean metallicity of globular clusters in two galaxies is identical. The differences in the observed frequency distribution of the parameters, in particular in the limits of general metallicity, are related to the random errors of photometrical measurements of globular clusters, considerably greater in the case of M31. Thereby the preference should be given to Hanes conclusion that globular clusters form a uniform population at least in two close systems. It should not be excepted that in other galaxies mean colour characteristics and hence metallicity of clusters may be of other type. Thus globular clusters related to the M31-NGC 205 satellite have somewhat minor B-V colour factors

  7. Young globular clusters in NGC 1316

    Science.gov (United States)

    Sesto, Leandro A.; Faifer, Favio R.; Smith Castelli, Analía V.; Forte, Juan C.; Escudero, Carlos G.

    2018-05-01

    We present multi-object spectroscopy of the inner zone of the globular cluster (GC) system associated with the intermediate-age merger remnant NGC 1316. Using the multi-object mode of the GMOS camera, we obtained spectra for 35 GCs. We find pieces of evidence that the innermost GCs of NGC 1316 rotate almost perpendicular to the stellar component of the galaxy. In a second stage, we determined ages, metallicities and α-element abundances for each GC present in the sample, through the measurement of different Lick/IDS indices and their comparison with simple stellar population models. We confirmed the existence of multiple GC populations associated with NGC 1316, where the presence of a dominant subpopulation of very young GCs, with an average age of 2.1 Gyr, metallicities between -0.5 < [Z/H] < 0.5 dex and α-element abundances in the range -0.2 < [α/Fe] < 0.3 dex, stands out. Several objects in our sample present subsolar values of [α/Fe] and a large spread of [Z/H] and ages. Some of these objects could actually be stripped nuclei, possibly accreted during minor merger events. Finally, the results have been analyzed with the aim of describing the different episodes of star formation and thus provide a more complete picture about the evolutionary history of the galaxy. We conclude that these pieces of evidence could indicate that this galaxy has cannibalized one or more gas-rich galaxies, where the last fusion event occurred about 2 Gyr ago.

  8. Evaluación de proteína C reactiva, velocidad de sedimentación globular, reticulocitos y ancho de distribución eritrocitaria, en niños menores de 5 años con anemia por déficit de hierro, en Cumaná, Venezuela | Evaluation of C reactive protein, erythrocyte sedimentation rate, reticulocytes and erithrocyte distribution width, in children under 5 years old with iron deficiency anemia, in Cumaná, Venezuela

    Directory of Open Access Journals (Sweden)

    Erika Hannaoui

    2017-11-01

    Full Text Available In order to evaluate C-reactive protein, rate of globular sedimentation, reticulocytes and erythrocyte distribution width, in infants with iron deficiency anemia, 114 children of both genders were studied, aged 0 to 5 years, who attended the pediatric consultation of three health centers in the city of Cumana, Sucre state. The infants were divided into three groups: 35 apparently healthy children formed the control group, 59 children enrolled in iron deficiency anemia and 20 children with anemia from other causes. Each infant was determined the levels of serum iron, ferritin, C-reactive protein (CRP, hemoglobin, hematocrit, hematimetric indices (MCHC and MCV, red cell distribution width (ADE, erythrocyte sedimentation rate (ESR and percentage reticulocyte. An ANOVA statistical analysis was applied to determine possible differences between the evaluated parameters. There were highly significant differences in the levels of iron, hemoglobin, hematocrit, MCHC, MCV and ADE among the three groups studied; while ferritin variables, reticulocytes, CRP and ESR showed no significant differences among these groups. The results allow to conclude that the determination of iron and hematological parameters are useful tools for diagnosis and for monitoring patients with iron deficiency anemia. It is recommended that the ADE be included within the profile of exams for such patients in order to assess the degree of anisocitosis in iron deficiency anemia, and thus lead doctors to a quick and accurate diagnosis as quickly as possible.

  9. Pulsar-irradiated stars in dense globular clusters

    Science.gov (United States)

    Tavani, Marco

    1992-01-01

    We discuss the properties of stars irradiated by millisecond pulsars in 'hard' binaries of dense globular clusters. Irradiation by a relativistic pulsar wind as in the case of the eclipsing millisecond pulsar PSR 1957+20 alter both the magnitude and color of the companion star. Some of the blue stragglers (BSs) recently discovered in dense globular clusters can be irradiated stars in binaries containing powerful millisecond pulsars. The discovery of pulsar-driven orbital modulations of BS brightness and color with periods of a few hours together with evidence for radio and/or gamma-ray emission from BS binaries would valuably contribute to the understanding of the evolution of collapsed stars in globular clusters. Pulsar-driven optical modulation of cluster stars might be the only observable effect of a new class of binary pulsars, i.e., hidden millisecond pulsars enshrouded in the evaporated material lifted off from the irradiated companion star.

  10. A new impedance based approach to test the activity of recombinant protein--Semaphorins as a test case.

    Science.gov (United States)

    Birger, Anastasya; Besser, Elazar; Reubinoff, Benjamin; Behar, Oded

    2015-10-01

    The biological activity of a recombinant protein is routinely measured using a bioassay such as an enzyme assay. However, many proteins have no enzymatic activity and in many cases it is difficult to devise a simple and reliable approach to test their activity. Semaphorins, Ephrins, Slits, Netrins or amylin-assisted proteins have numerous activities affecting many systems and cell types in the human body. Most of them are also able to induce rapid cytoskeleton changes at least in some cell types. We assumed therefore, that such proteins might be tested based on their ability to modulate the cytoskeleton. Here we tested a number of semaphorins in an impedance based label-free platform that allows for dynamic monitoring of subtle morphological and adhesive changes. This system has proved to be a very fast, sensitive and effective way to monitor and determine the activity of such proteins. Furthermore we showed that it is possible to customize a cell-protein system by transfecting the cells with specific receptors and test the cell response following the addition of the recombinant ligand protein. Since other protein families such as Ephrins and Netrins can also influence the cytoskeleton of some cells, this approach may be applicable to a large number of proteins. Copyright © 2015 Elsevier GmbH. All rights reserved.

  11. Seeing deconvolution of globular clusters in M31

    International Nuclear Information System (INIS)

    Bendinelli, O.; Zavatti, F.; Parmeggiani, G.; Djorgovski, S.

    1990-01-01

    The morphology of six M31 globular clusters is examined using seeing-deconvolved CCD images. The deconvolution techniques developed by Bendinelli (1989) are reviewed and applied to the M31 globular clusters to demonstrate the methodology. It is found that the effective resolution limit of the method is about 0.1-0.3 arcsec for CCD images obtained in FWHM = 1 arcsec seeing, and sampling of 0.3 arcsec/pixel. Also, the robustness of the method is discussed. The implications of the technique for future studies using data from the Hubble Space Telescope are considered. 68 refs

  12. Access to gram scale amounts of functional globular adiponectin from E. coli inclusion bodies by alkaline-shock solubilization.

    Science.gov (United States)

    Heiker, John T; Klöting, Nora; Blüher, Matthias; Beck-Sickinger, Annette G

    2010-07-16

    The adipose tissue derived protein adiponectin exerts anti-diabetic, anti-inflammatory and anti-atherosclerotic effects. Adiponectin serum concentrations are in the microgram per milliliter range in healthy humans and inversely correlate with obesity and metabolic disorders. Accordingly, raising circulating adiponectin levels by direct administration may be an intriguing strategy in the treatment of obesity-related metabolic disorders. However production of large amounts of recombinant adiponectin protein is a primary obstacle so far. Here, we report a novel method for large amount production of globular adiponectin from E. coli inclusion bodies utilizing an alkaline-shock solubilization method without chaotropic agents followed by precipitation of the readily renaturing protein. Precipitation of the mildly solubilized protein capitalizes on advantages of inclusion body formation. This approach of inclusion body protein recovery provides access to gram scale amounts of globular adiponectin with standard laboratory equipment avoiding vast dilution or dialysis steps to neutralize the pH and renature the protein, thus saving chemicals and time. The precipitated protein is readily renaturing in buffer, is of adequate purity without a chromatography step and shows biological activity in cultured MCF7 cells and significantly lowered blood glucose levels in mice with streptozotocin induced type 1 diabetes. Copyright 2010 Elsevier Inc. All rights reserved.

  13. Testing of disease-resistance of pokeweed antiviral protein gene ...

    African Journals Online (AJOL)

    Transformation of pokeweed antiviral protein gene (PAP) into plants was shown to improve plant resistance to several viruses or fungi pathogens with no much negative effect on plant growth. The non-virulent defective PAP inhibits only the virus but does not interfere with the host. A non-virulent defective PAP gene ...

  14. Possible systematic decreases in the age of globular clusters

    Energy Technology Data Exchange (ETDEWEB)

    Shi, X. [Univ. of Chicago, Chicago, IL (United States); Schramm, D. N. [Fermi National Accelerator Laboratory (FNAL), Batavia, IL (United States); Univ. of Chicago, Chicago, IL (United States); Dearborn, D. S.P. [Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States); Truran, J. W. [Univ. of Chicago, Chicago, IL (United States)

    1994-03-01

    The ages of globular clusters inferred from observations depends sensitively on assumptions like the initial helium abundance and the mass loss rate. A high helium abundance (e.g., Y\\approx0.28) or a mass loss rate of \\sim10^{-11}M_\\odot yr^{-1} near the main sequence turn-off region lowers the current age estimate from 14 Gyr to about 10--12 Gyr, significantly relaxing the constraints on the Hubble constant, allowing values as high as 60km/sec/Mpc for a universe with the critical density and 90km/sec/Mpc for a baryon-only universe. Possible mechanisms for the helium enhancement in globular clusters are discussed, as are arguments for an instability strip induced mass loss near the turn-off. Ages lower than 10 Gyr are not possible even with the operation of both of these mechanisms unless the initial helium abundance in globular clusters is >0.30, which would conflict with indirect measurements of helium abundances in globular clusters.

  15. Trazando la materia oscura con cúmulos globulares

    Science.gov (United States)

    Forte, J. C.

    Se describe la estrategia adoptada para mapear la distribución de materia oscura y bariónica en galaxias elípticas cuyos cúmulos globulares están siendo observados con los telescopios VLT y Gemini. Se ejemplifican los resultados con los datos obtenidos en el cúmulo de Fornax.

  16. BVRI CCD photometry of the globular cluster NGC 2808

    International Nuclear Information System (INIS)

    Alcaino, G.; Liller, W.; Alvarado, F.; Wenderoth, E.

    1990-01-01

    As a part of a continuing program, CCD color-magnitude diagrams are presented for the bright globular cluster NGC 2808 in the four colors comprising BVRI. From a comparison of four different CMDs with theoretical isochrones, an age of 16 + or - 2 Gyr is obtained, assuming a value for Fe/H near -1.3. 28 refs

  17. Supra-galactic colour patterns in globular cluster systems

    Science.gov (United States)

    Forte, Juan C.

    2017-07-01

    An analysis of globular cluster systems associated with galaxies included in the Virgo and Fornax Hubble Space Telescope-Advanced Camera Surveys reveals distinct (g - z) colour modulation patterns. These features appear on composite samples of globular clusters and, most evidently, in galaxies with absolute magnitudes Mg in the range from -20.2 to -19.2. These colour modulations are also detectable on some samples of globular clusters in the central galaxies NGC 1399 and NGC 4486 (and confirmed on data sets obtained with different instruments and photometric systems), as well as in other bright galaxies in these clusters. After discarding field contamination, photometric errors and statistical effects, we conclude that these supra-galactic colour patterns are real and reflect some previously unknown characteristic. These features suggest that the globular cluster formation process was not entirely stochastic but included a fraction of clusters that formed in a rather synchronized fashion over large spatial scales, and in a tentative time lapse of about 1.5 Gy at redshifts z between 2 and 4. We speculate that the putative mechanism leading to that synchronism may be associated with large scale feedback effects connected with violent star-forming events and/or with supermassive black holes.

  18. Integrated photometry of globular star clusters in the Vilnius system

    International Nuclear Information System (INIS)

    Zdanavichyus, K.V.

    1983-01-01

    Integrated colour indices in the Vilnius photometric system and newly determined colour excesses Esub(B-V) for 39 globular clusters are presented. It is shown that the coincidence of integrated spectral types are not a sufficient criterion for the identity of intrinsic colour indices of globular clusters. Relation of integrated colour indices with the slope of the giant branch S and with the horizontal branch morphological type D is investigated. Integrated colour indices of clusters with a blue horizontal branch show no correlation with either D or S. The increase of colour indices of the clusters of types D >= 4 correlates with the distribution of stars along the horizontal branch. Integrated photometry of globular star clusters in the Vilnius multicoloured photometric system permits to determine their colour excesses from some Q diagrams and normal colour index. Integral normal colour indexes and Q parameters for I globular star clusters of the Mironov group display small changes as compared to clusters of group 2. Colour indexes among star clusters having only red horizontal branches (D=7) change most considerably

  19. DARK MATTER HALOS IN GALAXIES AND GLOBULAR CLUSTER POPULATIONS

    Energy Technology Data Exchange (ETDEWEB)

    Hudson, Michael J.; Harris, Gretchen L. [Department of Physics and Astronomy, University of Waterloo, Waterloo, ON N2L 3G1 (Canada); Harris, William E., E-mail: mjhudson@uwaterloo.ca [Department of Physics and Astronomy, McMaster University, Hamilton, ON L8S 4M1 (Canada)

    2014-05-20

    We combine a new, comprehensive database for globular cluster populations in all types of galaxies with a new calibration of galaxy halo masses based entirely on weak lensing. Correlating these two sets of data, we find that the mass ratio η ≡ M {sub GCS}/M {sub h} (total mass in globular clusters, divided by halo mass) is essentially constant at (η) ∼ 4 × 10{sup –5}, strongly confirming earlier suggestions in the literature. Globular clusters are the only known stellar population that formed in essentially direct proportion to host galaxy halo mass. The intrinsic scatter in η appears to be at most 0.2 dex; we argue that some of this scatter is due to differing degrees of tidal stripping of the globular cluster systems between central and satellite galaxies. We suggest that this correlation can be understood if most globular clusters form at very early stages in galaxy evolution, largely avoiding the feedback processes that inhibited the bulk of field-star formation in their host galaxies. The actual mean value of η also suggests that about one-fourth of the initial gas mass present in protogalaxies collected into giant molecular clouds large enough to form massive, dense star clusters. Finally, our calibration of (η) indicates that the halo masses of the Milky Way and M31 are (1.2 ± 0.5) × 10{sup 12} M {sub ☉} and (3.9 ± 1.8) × 10{sup 12} M {sub ☉}, respectively.

  20. RETENTION OF STELLAR-MASS BLACK HOLES IN GLOBULAR CLUSTERS

    International Nuclear Information System (INIS)

    Morscher, Meagan; Umbreit, Stefan; Farr, Will M.; Rasio, Frederic A.

    2013-01-01

    Globular clusters should be born with significant numbers of stellar-mass black holes (BHs). It has been thought for two decades that very few of these BHs could be retained through the cluster lifetime. With masses ∼10 M ☉ , BHs are ∼20 times more massive than an average cluster star. They segregate into the cluster core, where they may eventually decouple from the remainder of the cluster. The small-N core then evaporates on a short timescale. This is the so-called Spitzer instability. Here we present the results of a full dynamical simulation of a globular cluster containing many stellar-mass BHs with a realistic mass spectrum. Our Monte Carlo simulation code includes detailed treatments of all relevant stellar evolution and dynamical processes. Our main finding is that old globular clusters could still contain many BHs at present. In our simulation, we find no evidence for the Spitzer instability. Instead, most of the BHs remain well mixed with the rest of the cluster, with only the innermost few tens of BHs segregating significantly. Over the 12 Gyr evolution, fewer than half of the BHs are dynamically ejected through strong binary interactions in the cluster core. The presence of BHs leads to long-term heating of the cluster, ultimately producing a core radius on the high end of the distribution for Milky Way globular clusters (and those of other galaxies). A crude extrapolation from our model suggests that the BH-BH merger rate from globular clusters could be comparable to the rate in the field.

  1. A New Globular Cluster in the Area of VVVX

    Science.gov (United States)

    Bica, E.; Minniti, D.; Bonatto, C.; Hempel, M.

    2018-06-01

    We communicate the discovery of a new globular cluster in the Galaxy that was first detected on WISE/2MASS images and is now confirmed with VVVX photometry. It is a Palomar-like cluster projected at ℓ = 359.15°, b = 5.73°, and may be related to the bulge. We derive an absolute magnitude of MV ≈ -3.3, thus being an underluminous globular cluster. Our analyses provide a reddening of E(B - V) = 1.08 ± 0.18 and a distance to the Sun d⊙ = 6.3 ± 1 kpc, which implies a current position in the bulge volume. The estimated metallicity is [Fe/H] = -1.5 ± 0.25. It adds to the recently discovered faint globular cluster (Minniti 22) and candidates found with VVV, building up expectations of ≈50 globular clusters yet to be discovered in the bulge. We also communicate the discovery of an old open cluster in the same VVVX tile as the globular cluster. The VVVX photometry provided E(B - V) = 0.62 ± 0.1, d⊙ = 7.6 ± 1 kpc, and an age of 1.5 ± 0.3 Gyr. With a height from the plane of ≈0.8 kpc, it adds to nine Gyr-class clusters recently discovered within 0.8 ⩽ Z ⩽ 2.2 kpc, as recently probed in the single VVV tile b201. We suggest that these findings may be disclosing the thick disk at the bulge, which so far has no open cluster counterpart, and hardly any individual star. Thus, the VVV and VVVX surveys are opening new windows for follow-up studies, to employ present and future generations of large aperture telescopes.

  2. The globular cluster systems of 54 Coma ultra-diffuse galaxies: statistical constraints from HST data

    Science.gov (United States)

    Amorisco, N. C.; Monachesi, A.; Agnello, A.; White, S. D. M.

    2018-04-01

    We use data from the HST Coma Cluster Treasury program to assess the richness of the globular cluster systems (GCSs) of 54 Coma ultra-diffuse galaxies (UDGs), 18 of which have a half-light radius exceeding 1.5 kpc. We use a hierarchical Bayesian method tested on a large number of mock data sets to account consistently for the high and spatially varying background counts in Coma. These include both background galaxies and intra-cluster globular clusters (ICGCs), which are disentangled from the population of member globular clusters (GCs) in a probabilistic fashion. We find no candidate for a GCS as rich as that of the Milky Way, our sample has GCSs typical of dwarf galaxies. For the standard relation between GCS richness and halo mass, 33 galaxies have a virial mass Mvir ≤ 1011 M⊙ at 90 per cent probability. Only three have Mvir > 1011 M⊙ with the same confidence. The mean colour and spread in colour of the UDG GCs are indistinguishable from those of the abundant population of ICGCs. The majority of UDGs in our sample are consistent with the relation between stellar mass and GC richness of `normal' dwarf galaxies. Nine systems, however, display GCSs that are richer by a factor of 3 or more (at 90 per cent probability). Six of these have sizes ≲1.4 kpc. Our results imply that the physical mechanisms responsible for the extended size of the UDGs and for the enhanced GC richness of some cluster dwarfs are at most weakly correlated.

  3. Understanding the central kinematics of globular clusters with simulated integrated-light IFU observations

    Science.gov (United States)

    Bianchini, Paolo; Norris, Mark A.; van de Ven, Glenn; Schinnerer, Eva

    2015-10-01

    The detection of intermediate-mass black holes in the centres of globular clusters is highly controversial, as complementary observational methods often deliver significantly different results. In order to understand these discrepancies, we develop a procedure to simulate integral field unit (IFU) observations of globular clusters: Simulating IFU Star Cluster Observations (SISCO). The inputs of our software are realistic dynamical models of globular clusters that are then converted in a spectral data cube. We apply SISCO to Monte Carlo cluster simulations with a realistic number of stars and concentrations. Using independent realizations of a given simulation we are able to quantify the stochasticity intrinsic to the problem of observing a partially resolved stellar population with integrated-light spectroscopy. We show that the luminosity-weighted IFU observations can be strongly biased by the presence of a few bright stars that introduce a scatter in the velocity dispersion measurements up to ≃40 per cent around the expected value, preventing any sound assessment of the central kinematic and a sensible interpretation of the presence/absence of an intermediate-mass black hole. Moreover, we illustrate that, in our mock IFU observations, the average kinematic tracer has a mass of ≃0.75 M⊙, only slightly lower than the mass of the typical stars examined in studies of resolved line-of-sight velocities of giant stars. Finally, in order to recover unbiased kinematic measurements we test different masking techniques that allow us to remove the spaxels dominated by bright stars, bringing the scatter down to a level of only a few per cent. The application of SISCO will allow us to investigate state-of-the-art simulations as realistic observations.

  4. Model test on the relationship feed energy and protein ratio to the production and quality of milk protein

    Science.gov (United States)

    Hartanto, R.; Jantra, M. A. C.; Santosa, S. A. B.; Purnomoadi, A.

    2018-01-01

    The purpose of this research was to find an appropriate relationship model between the feed energy and protein ratio with the amount of production and quality of milk proteins. This research was conducted at Getasan Sub-district, Semarang Regency, Central Java Province, Indonesia using 40 samples (Holstein Friesian cattle, lactation period II-III and lactation month 3-4). Data were analyzed using linear and quadratic regressions, to predict the production and quality of milk protein from feed energy and protein ratio that describe the diet. The significance of model was tested using analysis of variance. Coefficient of determination (R2), residual variance (RV) and root mean square prediction error (RMSPE) were reported for the developed equations as an indicator of the goodness of model fit. The results showed no relationship in milk protein (kg), milk casein (%), milk casein (kg) and milk urea N (mg/dl) as function of CP/TDN. The significant relationship was observed in milk production (L or kg) and milk protein (%) as function of CP/TDN, both in linear and quadratic models. In addition, a quadratic change in milk production (L) (P = 0.003), milk production (kg) (P = 0.003) and milk protein concentration (%) (P = 0.026) were observed with increase of CP/TDN. It can be concluded that quadratic equation was the good fitting model for this research, because quadratic equation has larger R2, smaller RV and smaller RMSPE than those of linear equation.

  5. A simple model for electrical charge in globular macromolecules and linear polyelectrolytes in solution

    Science.gov (United States)

    Krishnan, M.

    2017-05-01

    We present a model for calculating the net and effective electrical charge of globular macromolecules and linear polyelectrolytes such as proteins and DNA, given the concentration of monovalent salt and pH in solution. The calculation is based on a numerical solution of the non-linear Poisson-Boltzmann equation using a finite element discretized continuum approach. The model simultaneously addresses the phenomena of charge regulation and renormalization, both of which underpin the electrostatics of biomolecules in solution. We show that while charge regulation addresses the true electrical charge of a molecule arising from the acid-base equilibria of its ionizable groups, charge renormalization finds relevance in the context of a molecule's interaction with another charged entity. Writing this electrostatic interaction free energy in terms of a local electrical potential, we obtain an "interaction charge" for the molecule which we demonstrate agrees closely with the "effective charge" discussed in charge renormalization and counterion-condensation theories. The predictions of this model agree well with direct high-precision measurements of effective electrical charge of polyelectrolytes such as nucleic acids and disordered proteins in solution, without tunable parameters. Including the effective interior dielectric constant for compactly folded molecules as a tunable parameter, the model captures measurements of effective charge as well as published trends of pKa shifts in globular proteins. Our results suggest a straightforward general framework to model electrostatics in biomolecules in solution. In offering a platform that directly links theory and experiment, these calculations could foster a systematic understanding of the interrelationship between molecular 3D structure and conformation, electrical charge and electrostatic interactions in solution. The model could find particular relevance in situations where molecular crystal structures are not available or

  6. Surface Compositions of Red Giant Stars in Globular Clusters

    Science.gov (United States)

    Cheng, Eric; Lau, Marie; Smith, Graeme; Chen, Brian

    2018-01-01

    Globular clusters (GCs) are excellent “laboratories” to study the formation and evolution of our galaxy. In order to understand, more specifically, the chemical compositions and stellar evolution of the stars in GCs, we ask whether or not deep internal mixing occurs in red giants or if in fact the compositions come from the primordial interstellar medium or previous generations of stars. It has been discovered that as a star evolves up the red giant branch, the surface carbon abundance decreases, which is evidence of deep internal mixing. We questioned whether these processes also affect O or Na abundance as a star evolves. We collected measurement data of red giants from GCs out of academic journals and sorted the data into catalogs. Then, we plotted the catalogs into figures, comparing surface O and Na each with stellar luminosity. Statistical tests were ran to quantify the amount of correlation between the variables. Out of 27 GCs, we concluded that eight show a positive correlation between Na and luminosity, and two show a negative correlation between O and luminosity. Properties of GCs were compared to determine if chemical distribution in stars depends on GCs as the self-enrichment scenario suggests. We created histograms of sodium distribution to test for bimodality to examine if there are separate trends in each GC. In six GCs, two different sequences of red giants appear for Na versus luminosity, suggesting evidence that the depth of mixing may differ among each red giant in a GC. This study has provided new evidence that the changing chemical abundances on the surfaces of red giants can be due to stellar evolutionary effects and deep internal mixing, which may not necessarily depend on the GC and may differ in depth among each red giant. Through this study, we learn more about stellar evolution which will eventually help us understand the origins of our universe. Most of this work was carried out by high school students working under the auspices of

  7. Ruprecht 106 - A young metal-poor Galactic globular cluster

    International Nuclear Information System (INIS)

    Buonanno, R.; Buscema, G.; Fusi Pecci, F.; Richer, H.B.; Fahlman, G.G.

    1990-01-01

    The first CCD photometric survey in the Galactic globular cluster Ruprecht 106 has been performed. The results show that Ruprecht 106 is a metal-poor cluster with (Fe/H) about -2 located at about 25 kpc from the Galactic center. A sizable, high centrally concentrated population of blue stragglers was detected. Significant differences in the positions of the turnoffs in the color-magnitude diagram are found compared to those in metal-poor clusters. The cluster appears younger than other typical metal-poor Galactic globulars by about 4-5 Gyr; if true, this object would represent the first direct proof of the existence of a significant age spread among old, very metal-poor clusters. 51 refs

  8. Is age really the second parameter in globular clusters?

    International Nuclear Information System (INIS)

    Vandenberg, D.A.; Durrell, P.R.

    1990-01-01

    From the close similarity of the magnitude difference between the tip of the red giant branch and the turnoff in the Fe/H = about -1.3 globular cluster NGC 288, NGC 362, and M5, it is inferred that the ages of these three systems (and Palomar 5, whose horizonal branch is used to define its distance relative to the others) are not detectably different. An identical conclusion, by similar means, is reached for the Fe/H = about -2.1 globular clusters M15, M30, M68, and M92. Several recent claims that age is responsible for the wide variation in horizontal-branch morphology among clusters of the same metal abundance are not supported. 73 refs

  9. Globular cluster seeding by primordial black hole population

    Energy Technology Data Exchange (ETDEWEB)

    Dolgov, A. [ITEP, Bol. Cheremushkinsaya ul., 25, 117218 Moscow (Russian Federation); Postnov, K., E-mail: dolgov@fe.infn.it, E-mail: kpostnov@gmail.com [Sternberg Astronomical Institute, Moscow M.V. Lomonosov State University, Universitetskij pr., 13, Moscow 119234 (Russian Federation)

    2017-04-01

    Primordial black holes (PBHs) that form in the early Universe in the modified Affleck-Dine (AD) mechanism of baryogenesis should have intrinsic log-normal mass distribution of PBHs. We show that the parameters of this distribution adjusted to provide the required spatial density of massive seeds (≥ 10{sup 4} M {sub ⊙}) for early galaxy formation and not violating the dark matter density constraints, predict the existence of the population of intermediate-mass PBHs with a number density of 0∼ 100 Mpc{sup −3}. We argue that the population of intermediate-mass AD PBHs can also seed the formation of globular clusters in galaxies. In this scenario, each globular cluster should host an intermediate-mass black hole with a mass of a few thousand solar masses, and should not obligatorily be immersed in a massive dark matter halo.

  10. On tidal radius determination for a globular cluster

    International Nuclear Information System (INIS)

    Ninkovic, S.

    1985-01-01

    A tidal radius determination for a globular cluster based on its density minimum, which is caused by the galactic tidal forces and derivable from a model of the Galaxy, is proposed. Results obtained on the basis of the Schmidt model for two clusters are in a satisfactory agreement with those obtained earlier by means of other methods. A mass determination for the clusters through the tidal radius, when the latter one is identified with the cluster perigalactic distance, yields unusually large mass values. Probably, the tidal radius should be identified with the instantaneous galactocentric distance. Use of models more recent than the Schmidt one indicates that a globular cluster may contain a significant portion of an invisible interstellar matter. (author)

  11. Rates of collapse and evaporation of globular clusters

    Science.gov (United States)

    Hut, Piet; Djorgovski, S.

    1992-01-01

    Observational estimates of the dynamical relaxation times of Galactic globular clusters are used here to estimate the present rate at which core collapse and evaporation are occurring in them. A core collapse rate of 2 +/- 1 per Gyr is found, which for a Galactic age of about 12 Gyr agrees well with the fact that 27 clusters have surface brightness profiles with the morphology expected for the postcollapse phase. A destruction and evaporation rate of 5 +/- 3 per Gyr is found, suggesting that a significant fraction of the Galaxy's original complement of globular clusters have perished through the combined effects of mechanisms such as relaxation-driven evaporation and shocking due to interaction with the Galactic disk and bulge.

  12. Color maps of X-ray globular clusters

    International Nuclear Information System (INIS)

    Bailyn, C.D.; Grindlay, J.E.; Cohn, H.; Lugger, P.M.

    1988-01-01

    The results of a search for optical counterparts to X-ray sources in six globular clusters, 47 Tuc, NGC 1851, NGC 6441, NGC 6624, NGC 6712, and M15, are reported. Maps of the U-B color of the central regions of the clusters were prepared. A candidate for the optical counterpart of the source in NGC 6712 was found, along with a blue region near the X-ray source in 47 Tuc. Upper limits on the colors and magnitudes of possible optical counterparts are reported for the other three clusters. The use of color maps to determine color gradients in globular clusters is explored. It is found that, while such gradients do exist and vary from cluster to cluster, they can be explained by crowding effects. Crude limits are placed on the excess populations of blue objects such as CVs, which have been postulated to be concentrated in the centers of dense clusters. 32 references

  13. MORE REMOTE GLOBULAR CLUSTERS IN THE OUTER HALO OF M31

    International Nuclear Information System (INIS)

    Di Tullio Zinn, Graziella; Zinn, Robert

    2013-01-01

    We searched the Sloan Digital Sky Survey for outer halo globular clusters (GCs) around M31. Our search of non-stellar objects, within the limits of 0.3 ≤ (g – i) 0 ≤ 1.5 and 14.0 ≤ r 0 ≤ 19.0 concentrated in some remote areas of the extended halo, to a maximum projected distance of 240 kpc, for a total of approximately 200 deg 2 . Another ∼50 deg 2 , ∼5-75 kpc from M31, were surveyed as test areas. In these areas, we identified 39 GCs and 2 GC candidates, 84% of the previously known GCs (93% of the 'classical GCs' and 40% of the 'halo extended clusters', on the cluster classification scheme of Huxor et al.). For the entire survey, we visually inspected 78,516 objects for morphological evidence of cluster status, and we identified 18 new clusters, and 75 candidate clusters. The new clusters include 15 classical globulars and 3 clusters of lower density. Six of the clusters reside in the remote areas of the outer halo, beyond projected distances of 100 kpc. Previously, only MGC1 was found beyond this limit at 117 kpc. The farthest cluster discovered in this survey lies at a projected radius of 158 kpc from M31, assuming that the M31 distance is 780 kpc.

  14. An observational study of disk-population globular clusters

    International Nuclear Information System (INIS)

    Armandroff, T.E.

    1988-01-01

    Integrated-light spectroscopy was obtained for twenty-seven globular clusters at the Ca II infrared triplet. Line strengths and radial velocities were measured from the spectra. For the well-studied clusters in the sample, the strength of the CA II lines is very well correlated with previous metallicity estimates obtained using a variety of techniques. The greatly reduced effect of interstellar extinction at these wavelengths compared to the blue region of the spectrum has permitted observations of some of the most heavily reddened clusters in the Galaxy. For several such clusters, the Ca II triplet metallicities are in poor agreement with metallicity estimates from infrared photometry by Malkan. Color-magnitude diagrams were constructed for six previously unstudied metal-rich globular clusters and for the well-studied cluster 47 Tuc. The V magnitudes of the horizontal branch stars in the six clusters are in poor agreement with previous estimates based on secondary methods. The horizontal branch morphologies and reddenings of the program clusters were also determined. Using the improved set of metallicities, radial velocities, and distance moduli, the spatial distribution, kinematics, and metallicity distribution of the Galactic globulars were analyzed. The revised data supports Zinn's conclusion that the metal-rich clusters form a highly flattened, rapidly rotating disk system, while the metal-poor clusters make up the familiar, spherically distributed, slowly rotating halo population. The scale height, metallicity distribution, and kinematics of the metal-rich globulars are in good agreement with those of the stellar thick disk. Luminosity functions were constructed, and no significant difference is found between disk and halo samples. Metallicity gradients seem to be present in the disk cluster system. The implications of these results for the formation and evol

  15. Evolution of redback radio pulsars in globular clusters

    Science.gov (United States)

    Benvenuto, O. G.; De Vito, M. A.; Horvath, J. E.

    2017-02-01

    Context. We study the evolution of close binary systems composed of a normal, intermediate mass star and a neutron star considering a chemical composition typical of that present in globular clusters (Z = 0.001). Aims: We look for similarities and differences with respect to solar composition donor stars, which we have extensively studied in the past. As a definite example, we perform an application on one of the redbacks located in a globular cluster. Methods: We performed a detailed grid of models in order to find systems that represent the so-called redback binary radio pulsar systems with donor star masses between 0.6 and 2.0 solar masses and orbital periods in the range 0.2-0.9 d. Results: We find that the evolution of these binary systems is rather similar to those corresponding to solar composition objects, allowing us to account for the occurrence of redbacks in globular clusters, as the main physical ingredient is the irradiation feedback. Redback systems are in the quasi-RLOF state, that is, almost filling their corresponding Roche lobe. During the irradiation cycle the system alternates between semi-detached and detached states. While detached the system appears as a binary millisecond pulsar, called a redback. Circumstellar material, as seen in redbacks, is left behind after the previous semi-detached phase. Conclusions: The evolution of binary radio pulsar systems considering irradiation successfully accounts for, and provides a way for, the occurrence of redback pulsars in low-metallicity environments such as globular clusters. This is the case despite possible effects of the low metal content of the donor star that could drive systems away from redback configuration.

  16. Globular Cluster Candidates for Hosting a Central Black Hole

    Science.gov (United States)

    Noyola, Eva

    2009-07-01

    We are continuing our study of the dynamical properties of globular clusters and we propose to obtain surface brightness profiles for high concentration clusters. Our results to date show that the distribution of central surface brightness slopes do not conform to standard models. This has important implications for how they form and evolve, and suggest the possible presence of central intermediate-mass black holes. From our previous archival proposals {AR-9542 and AR-10315}, we find that many high concentration globular clusters do not have flat cores or steep central cusps, instead they show weak cusps. Numerical simulations suggest that clusters with weak cusps may harbor intermediate-mass black holes and we have one confirmation of this connection with omega Centauri. This cluster shows a shallow cusp in its surface brightness profile, while kinematical measurements suggest the presence of a black hole in its center. Our goal is to extend these studies to a sample containing 85% of the Galactic globular clusters with concentrations higher than 1.7 and look for objects departing from isothermal behavior. The ACS globular cluster survey {GO-10775} provides enough objects to have an excellent coverage of a wide range of galactic clusters, but it contains only a couple of the ones with high concentration. The proposed sample consists of clusters whose light profile can only be adequately measured from space-based imaging. This would take us close to completeness for the high concentration cases and therefore provide a more complete list of candidates for containing a central black hole. The dataset will also be combined with our existing kinematic measurements and enhanced with future kinematic studies to perform detailed dynamical modeling.

  17. Imprint of galaxy formation and evolution on globular cluster properties

    OpenAIRE

    Bekki, Kenji

    2006-01-01

    We discuss the origin of physical properties of globular cluster systems (GCSs) in galaxies in terms of galaxy formation and evolution processes. Based on numerical simulations of dynamical evolution of GCSs in galaxies, we particularly discuss (1) the origin of radial density profiles of GCSs, (2) kinematics of GCSs in elliptical galaxies, (3) transformation from nucleated dwarf galaxies into GCs (e.g., omega Centauri), and (4) the origin of GCSs in the Large Magellanic Cloud (LMC).

  18. Globular adiponectin controls insulin-mediated vasoreactivity in muscle through AMPKα2

    DEFF Research Database (Denmark)

    de Boer, Michiel P; Meijer, Rick I; Richter, Erik

    2016-01-01

    Decreased tissue perfusion increases the risk of developing insulin resistance and cardiovascular disease in obesity, and decreased levels of globular adiponectin (gAdn) have been proposed to contribute to this risk. We hypothesized that gAdn controls insulin's vasoactive effects through AMP......-activated protein kinase (AMPK), specifically its α2 subunit, and studied the mechanisms involved. In healthy volunteers, we found that decreased plasma gAdn levels in obese subjects associate with insulin resistance and reduced capillary perfusion during hyperinsulinemia. In cultured human microvascular...... endothelial cells (HMEC), gAdn increased AMPK activity. In isolated muscle resistance arteries gAdn uncovered insulin-induced vasodilation by selectively inhibiting insulin-induced activation of ERK1/2, and the AMPK inhibitor compound C as well as genetic deletion of AMPKα2 blunted insulin...

  19. Theoretical stellar luminosity functions and globular cluster ages and compositions

    International Nuclear Information System (INIS)

    Ratcliff, S.J.

    1985-01-01

    The ages and chemical compositions of the stars in globular clusters are of great interest, particularly because age estimates from the well-known exercise of fitting observed color-magnitude diagrams to theoretical predictions tend to yield ages in excess of the Hubble time (an estimate to the age of the Universe) in standard cosmological models, for currently proposed high values of Hubble's constant (VandenBerg 1983). Relatively little use has been made of stellar luminosity functions of the globular clusters, for which reliable observations are now becoming available, to constrain the ages or compositions. The comparison of observed luminosity functions to theoretical ones allows one to take advantage of information not usually used, and has the advantage of being relatively insensitive to our lack of knowledge of the detailed structure of stellar envelopes and atmospheres. A computer program was developed to apply standard stellar evolutionary theory, using the most recently available input physics (opacities, nuclear reaction rates), to the calculation of the evolution of low-mass Population II stars. An algorithm for computing luminosity functions from the evolutionary tracks was applied to sets of tracks covering a broad range of chemical compositions and ages, such as may be expected for globular clusters

  20. MOCK OBSERVATIONS OF BLUE STRAGGLERS IN GLOBULAR CLUSTER MODELS

    International Nuclear Information System (INIS)

    Sills, Alison; Glebbeek, Evert; Chatterjee, Sourav; Rasio, Frederic A.

    2013-01-01

    We created artificial color-magnitude diagrams of Monte Carlo dynamical models of globular clusters and then used observational methods to determine the number of blue stragglers in those clusters. We compared these blue stragglers to various cluster properties, mimicking work that has been done for blue stragglers in Milky Way globular clusters to determine the dominant formation mechanism(s) of this unusual stellar population. We find that a mass-based prescription for selecting blue stragglers will select approximately twice as many blue stragglers than a selection criterion that was developed for observations of real clusters. However, the two numbers of blue stragglers are well-correlated, so either selection criterion can be used to characterize the blue straggler population of a cluster. We confirm previous results that the simplified prescription for the evolution of a collision or merger product in the BSE code overestimates their lifetimes. We show that our model blue stragglers follow similar trends with cluster properties (core mass, binary fraction, total mass, collision rate) as the true Milky Way blue stragglers as long as we restrict ourselves to model clusters with an initial binary fraction higher than 5%. We also show that, in contrast to earlier work, the number of blue stragglers in the cluster core does have a weak dependence on the collisional parameter Γ in both our models and in Milky Way globular clusters

  1. Interdependence of the rad50 hook and globular domain functions.

    Science.gov (United States)

    Hohl, Marcel; Kochańczyk, Tomasz; Tous, Cristina; Aguilera, Andrés; Krężel, Artur; Petrini, John H J

    2015-02-05

    Rad50 contains a conserved Zn(2+) coordination domain (the Rad50 hook) that functions as a homodimerization interface. Hook ablation phenocopies Rad50 deficiency in all respects. Here, we focused on rad50 mutations flanking the Zn(2+)-coordinating hook cysteines. These mutants impaired hook-mediated dimerization, but recombination between sister chromatids was largely unaffected. This may reflect that cohesin-mediated sister chromatid interactions are sufficient for double-strand break repair. However, Mre11 complex functions specified by the globular domain, including Tel1 (ATM) activation, nonhomologous end joining, and DNA double-strand break end resection were affected, suggesting that dimerization exerts a broad influence on Mre11 complex function. These phenotypes were suppressed by mutations within the coiled-coil and globular ATPase domains, suggesting a model in which conformational changes in the hook and globular domains are transmitted via the extended coils of Rad50. We propose that transmission of spatial information in this manner underlies the regulation of Mre11 complex functions. Copyright © 2015 Elsevier Inc. All rights reserved.

  2. Implications of protein polymorphism on protein phase behaviour

    NARCIS (Netherlands)

    Stegen, J.; Schoot, van der P.P.A.M.

    2015-01-01

    The phase behaviour of small globular proteins is often modeled by approximating them as spherical particles with fixed internal structure. However, changes in the local environment of a protein can lead to changes in its conformation rendering this approximation invalid. We present a simple

  3. Protein scissors: Photocleavage of proteins at specific locations

    Indian Academy of Sciences (India)

    Unknown

    Binding of ligands to globular proteins at hydrophobic cavities while making specific ... ched to a PTI model A1010 monochromator. UV cut-off filter ..... >1:1 stoichiometry (protein to ligand), the binding equilibrium favors the thermo- dynamically ...

  4. Globular adiponectin induces a pro-inflammatory response in human astrocytic cells

    International Nuclear Information System (INIS)

    Wan, Zhongxiao; Mah, Dorrian; Simtchouk, Svetlana; Klegeris, Andis; Little, Jonathan P.

    2014-01-01

    Highlights: • Adiponectin receptors are expressed in human astrocytes. • Globular adiponectin induces secretion of IL-6 and MCP-1 from cultured astrocytes. • Adiponectin may play a pro-inflammatory role in astrocytes. - Abstract: Neuroinflammation, mediated in part by activated brain astrocytes, plays a critical role in the development of neurodegenerative disorders, including Alzheimer’s disease (AD). Adiponectin is the most abundant adipokine secreted from adipose tissue and has been reported to exert both anti- and pro-inflammatory effects in peripheral tissues; however, the effects of adiponectin on astrocytes remain unknown. Shifts in peripheral concentrations of adipokines, including adiponectin, could contribute to the observed link between midlife adiposity and increased AD risk. The aim of the present study was to characterize the effects of globular adiponectin (gAd) on pro-inflammatory cytokine mRNA expression and secretion in human U373 MG astrocytic cells and to explore the potential involvement of nuclear factor (NF)-κB, p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK)1/2, c-Jun N-terminal kinase (JNK) and phosphatidylinositide 3-kinases (PI3 K) signaling pathways in these processes. We demonstrated expression of adiponectin receptor 1 (adipoR1) and adipoR2 in U373 MG cells and primary human astrocytes. gAd induced secretion of interleukin (IL)-6 and monocyte chemoattractant protein (MCP)-1, and gene expression of IL-6, MCP-1, IL-1β and IL-8 in U373 MG cells. Using specific inhibitors, we found that NF-κB, p38MAPK and ERK1/2 pathways are involved in gAd-induced induction of cytokines with ERK1/2 contributing the most. These findings provide evidence that gAd may induce a pro-inflammatory phenotype in human astrocytes

  5. Globular adiponectin induces a pro-inflammatory response in human astrocytic cells

    Energy Technology Data Exchange (ETDEWEB)

    Wan, Zhongxiao; Mah, Dorrian; Simtchouk, Svetlana [School of Health and Exercise Sciences, University of British Columbia Okanagan, Kelowna, BC (Canada); Klegeris, Andis [Department of Biology, University of British Columbia Okanagan, Kelowna, BC (Canada); Little, Jonathan P., E-mail: jonathan.little@ubc.ca [School of Health and Exercise Sciences, University of British Columbia Okanagan, Kelowna, BC (Canada)

    2014-03-28

    Highlights: • Adiponectin receptors are expressed in human astrocytes. • Globular adiponectin induces secretion of IL-6 and MCP-1 from cultured astrocytes. • Adiponectin may play a pro-inflammatory role in astrocytes. - Abstract: Neuroinflammation, mediated in part by activated brain astrocytes, plays a critical role in the development of neurodegenerative disorders, including Alzheimer’s disease (AD). Adiponectin is the most abundant adipokine secreted from adipose tissue and has been reported to exert both anti- and pro-inflammatory effects in peripheral tissues; however, the effects of adiponectin on astrocytes remain unknown. Shifts in peripheral concentrations of adipokines, including adiponectin, could contribute to the observed link between midlife adiposity and increased AD risk. The aim of the present study was to characterize the effects of globular adiponectin (gAd) on pro-inflammatory cytokine mRNA expression and secretion in human U373 MG astrocytic cells and to explore the potential involvement of nuclear factor (NF)-κB, p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK)1/2, c-Jun N-terminal kinase (JNK) and phosphatidylinositide 3-kinases (PI3 K) signaling pathways in these processes. We demonstrated expression of adiponectin receptor 1 (adipoR1) and adipoR2 in U373 MG cells and primary human astrocytes. gAd induced secretion of interleukin (IL)-6 and monocyte chemoattractant protein (MCP)-1, and gene expression of IL-6, MCP-1, IL-1β and IL-8 in U373 MG cells. Using specific inhibitors, we found that NF-κB, p38MAPK and ERK1/2 pathways are involved in gAd-induced induction of cytokines with ERK1/2 contributing the most. These findings provide evidence that gAd may induce a pro-inflammatory phenotype in human astrocytes.

  6. On the Absolute Age of the Globular Cluster M92

    Science.gov (United States)

    Di Cecco, A.; Becucci, R.; Bono, G.; Monelli, M.; Stetson, P. B.; Degl'Innocenti, S.; Prada Moroni, P. G.; Nonino, M.; Weiss, A.; Buonanno, R.; Calamida, A.; Caputo, F.; Corsi, C. E.; Ferraro, I.; Iannicola, G.; Pulone, L.; Romaniello, M.; Walker, A. R.

    2010-09-01

    We present precise and deep optical photometry of the globular M92. Data were collected in three different photometric systems: Sloan Digital Sky Survey (g‧, r‧, i‧, and z‧ MegaCam at CFHT), Johnson-Kron-Cousins (B, V, and I; various ground-based telescopes), and Advanced Camera for Surveys (ACS) Vegamag (F475W, F555W, and F814W; Hubble Space Telescope). Special attention was given to the photometric calibration, and the precision of the ground-based data is generally better than 0.01 mag. We computed a new set of α-enhanced evolutionary models accounting for the gravitational settling of heavy elements at fixed chemical composition ([α/Fe] = +0.3, [Fe/H] = -2.32 dex, and Y = 0.248). The isochrones—assuming the same true distance modulus (μ = 14.74 mag), the same reddening [E(B - V) = 0.025 ± 0.010 mag], and the same reddening law—account for the stellar distribution along the main sequence and the red giant branch in different color-magnitude diagrams (i‧, g‧ - i‧ i‧, and g‧ - r‧ i‧, g‧ - z‧ I, and B - I and F814W and F475W-F814W). The same outcome applies to the comparison between the predicted zero-age horizontal-branch (ZAHB) and the HB stars. We also found a cluster age of 11 ± 1.5 Gyr, in good agreement with previous estimates. The error budget accounts for uncertainties in the input physics and the photometry. To test the possible occurrence of CNO-enhanced stars, we also computed two sets of α- and CNO-enhanced (by a factor of 3) models, both at fixed total metallicity ([M/H] = -2.10 dex) and at fixed iron abundance. We found that the isochrones based on the former set give the same cluster age (11 ± 1.5 Gyr) as the canonical α-enhanced isochrones. The isochrones based on the latter set also give a similar cluster age (10 ± 1.5 Gyr). These findings support previous results concerning the weak sensitivity of cluster isochrones to CNO-enhanced chemical mixtures. This paper makes use of data obtained from the Isaac Newton

  7. Effect of the horizontal branch on the colours of globular clusters

    Energy Technology Data Exchange (ETDEWEB)

    Sil' chenko, O K [Moskovskij Gosudarstvennyj Univ. (USSR). Gosudarstvennyj Astronomicheskij Inst. ' ' GAISh' '

    1963-05-01

    The influence of the horizontal branch (HB) on the integral UBV colours of globular clusters is studied by means of statistical analysis of the colour-magnitude diagram catalogue for globular clusters of our Galaxy. The colour correction for HB is shown to be always negative. It turns out to be small for m. tal-rich globular clusters ((Fe/H)>-1.1) and independent on the HB shape for metal-poor ones.

  8. Effect of the horizontal branch on the colours of globular clusters

    International Nuclear Information System (INIS)

    Sil'chenko, O.K.

    1963-01-01

    The influence of the horizontal branch (HB) on the integral UBV colours of globular clusters is studied by means of statistical analysis of the colour-magnitude diagram catalogue for globular clusters of our Galaxy. The colour correction for HB is shown to be always negative. It turns out to be small for m. tal-rich globular clusters ([Fe/H]>-1.1) and independent on the HB shape for metal-poor ones

  9. Statistical mechanics of protein solutions

    NARCIS (Netherlands)

    Prinsen, P.

    2007-01-01

    We study theoretically thermodynamic properties of spherical globular proteins in aqueous solution with added monovalent salt. We show how one can determine an effective interaction potential between the proteins from experimental data as a function of salt concentration and we apply this to the

  10. Use of recombinant purified protein derivative (PPD) antigens as specific skin test for tuberculosis.

    Science.gov (United States)

    Stavri, Henriette; Bucurenci, Nadia; Ulea, Irina; Costache, Adriana; Popa, Loredana; Popa, Mircea Ioan

    2012-11-01

    Purified protein derivative (PPD) is currently the only available skin test reagent used worldwide for the diagnosis of tuberculosis (TB). The aim of this study was to develop a Mycobacterium tuberculosis specific skin test reagent, without false positive results due to Bacillus Calmette-Guerin (BCG) vaccination using recombinant antigens. Proteins in PPD IC-65 were analyzed by tandem mass spectrometry and compared to proteins in M. tuberculosis culture filtrate; 54 proteins were found in common. Top candidates MPT64, ESAT 6, and CFP 10 were overexpressed in Escherichia coli expression strains and purified as recombinant proteins. To formulate optimal immunodiagnostic PPD cocktails, the antigens were evaluated by skin testing guinea pigs sensitized with M. tuberculosis H37Rv and BCG. For single antigens and a cocktail mixture of these antigens, best results were obtained using 3 μg/0.1 ml, equivalent to 105 TU (tuberculin units). Each animal was simultaneously tested with PPD IC-65, 2 TU/0.1 ml, as reference. Reactivity of the multi-antigen cocktail was greater than that of any single antigen. The skin test results were between 34.3 and 76.6 per cent the level of reactivity compared to that of the reference when single antigens were tested and 124 per cent the level of reactivity compared to the reference for the multi-antigen cocktail. Our results showed that this specific cocktail could represent a potential candidate for a new skin diagnostic test for TB.

  11. SEARCH FOR PULSED {gamma}-RAY EMISSION FROM GLOBULAR CLUSTER M28

    Energy Technology Data Exchange (ETDEWEB)

    Wu, J. H. K.; Kong, A. K. H.; Huang, R. H. H.; Tam, P. H. T. [Institute of Astronomy and Department of Physics, National Tsing Hua University, Hsinchu, Taiwan (China); Hui, C. Y. [Department of Astronomy and Space Science, Chungnam National University, Daejeon (Korea, Republic of); Wu, E. M. H.; Takata, J.; Cheng, K. S., E-mail: wuhkjason@gmail.com, E-mail: cyhui@cnu.ac.kr [Department of Physics, University of Hong Kong, Pokfulam Road (Hong Kong)

    2013-03-10

    Using the data from the Large Area Telescope on board the Fermi Gamma-ray Space Telescope, we have searched for {gamma}-ray pulsations from the direction of the globular cluster M28 (NGC 6626). We report the discovery of a signal with a frequency consistent with that of the energetic millisecond pulsar (MSP) PSR B1821-24 in M28. A weighted H-test test statistic of 28.8 is attained, which corresponds to a chance probability of {approx}10{sup -5} (4.3{sigma} detection). With a phase-resolved analysis, the pulsed component is found to contribute {approx}25% of the total observed {gamma}-ray emission from the cluster. However, the unpulsed level provides a constraint for the underlying MSP population and the fundamental plane relations for the scenario of inverse Compton scattering. Follow-up timing observations in radio/X-ray are encouraged to further investigate this periodic signal candidate.

  12. Anti-helium flux as a signature for antimatter globular clusters in our galaxy

    International Nuclear Information System (INIS)

    Belotskij, K.M.; Golubkov, Yu.A.; Khlopov, M.Yu.; Konoplich, R.V.; Sakharov, A.S.

    2000-01-01

    The alpha magnetic spectrometer experiment is shown to be sensitive to test the hypothesis on the existence of antimatter globular cluster in our Galaxy. The hypothesis follows from the analysis of possible tests for the mechanisms of baryosynthesis and uses antimatter domain in the matter domain Universe as the probe for the physics underlaying the origin of the matter. The interval of masses for the antimatter in our Galaxy is fixed from below by the condition of antimatter domain survival in the matter dominated Universe and from above by the observed gamma-ray flux. For this interval the expected fluxes of anti-helium-3 and anti-helium-4 are calculated with the account of their interaction with the matter in the Galaxy [ru

  13. Anti-helium flux as a signature for antimatter globular clusters in our galaxy

    International Nuclear Information System (INIS)

    Belotsky, K.M.; Golubkov, Yu.A.; Khlopov, M.Yu.; Konoplich, R.V.; Sakharov, A.S.

    2000-01-01

    The Alpha Magnetic Spectrometer experiment is shown to be sensitive to test the hypothesis on the existence of antimatter globular cluster in our Galaxy. The hypothesis follows from the analysis of possible tests for the mechanisms of baryosynthesis and uses antimatter domains in the matter-dominated Universe as the probe for the physics underlying the origin of matter. The interval of masses for the antimatter in our Galaxy is fixed from below by the condition of antimatter domain survival in the matter-dominated Universe and from above by the observed gamma-ray flux. For this interval, the expected fluxes of anti-helium-3 and anti-helium-4 are calculated with account for their interaction with the matter in the Galaxy

  14. Self-consistent field theory of protein adsorption in a non-Gaussian polyelectrolyte brush

    NARCIS (Netherlands)

    Biesheuvel, P.M.; Leermakers, F.A.M.; Stuart, M.A.C.

    2006-01-01

    To describe adsorption of globular protein molecules in a polyelectrolyte brush we use the strong-stretching approximation of the Edwards self-consistent field equation, combined with corrections for a non-Gaussian brush. To describe chemical potentials in this mixture of (globular) species of

  15. Problem-Solving Test: RNA and Protein Synthesis in Bacteriophage-Infected "E. coli" Cells

    Science.gov (United States)

    Szeberenyi, Jozsef

    2008-01-01

    The classic experiment presented in this problem-solving test was designed to identify the template molecules of translation by analyzing the synthesis of phage proteins in "Escherichia coli" cells infected with bacteriophage T4. The work described in this test led to one of the most seminal discoveries of early molecular biology: it dealt a…

  16. Problem-Solving Test: Analysis of DNA Damage Recognizing Proteins in Yeast and Human Cells

    Science.gov (United States)

    Szeberenyi, Jozsef

    2013-01-01

    The experiment described in this test was aimed at identifying DNA repair proteins in human and yeast cells. Terms to be familiar with before you start to solve the test: DNA repair, germline mutation, somatic mutation, inherited disease, cancer, restriction endonuclease, radioactive labeling, [alpha-[superscript 32]P]ATP, [gamma-[superscript…

  17. A dip-slide test for bacteriuria in protein-calorie malnutrition ...

    African Journals Online (AJOL)

    A simple dip-slide test was used to determine the incidence of bacteriuria in children suffering from protein-calorie malnutrition. Bacteriuria was found to be equally common in patients suffering from kwashiorkor and atrophic malnutrition and in a control group with normal nutritional status. The test is inexpensive and has a ...

  18. Immunization with influenza virus hemagglutinin globular region containing the receptor-binding pocket.

    Science.gov (United States)

    Jeon, Sung Ho; Arnon, Ruth

    2002-01-01

    The globular region of hemagglutinin (residues 91-261) membrane glycoprotein of influenza virus that encompasses the binding zone to the oligosaccharide receptor of target cells has been cloned by reverse transcriptase-polymerase chain reaction (RT-PCR). This protein segment (denoted HA91-261 peptide) induced significant immune response in mice. The serum antibodies and lung homogenates from the immunized mice cross-reacted with native virus particles. The cellular immunity was manifested by proliferative splenocyte responses and cytokine release indicating T helper type 1 activity. The plasmid DNA containing this segment (denoted pHA91-261) provoked, in addition, a significant cytotoxic T lymphocyte (CTL) response, whereas the HA91-261 protein fragment led to no such response. Both the DNA and the protein fragment of HA91-261 induced significant protection against viral challenge, although the immune response they induce might be along different pathways. Interestingly, the combined DNA priming-protein boosting immunization regimen did not induce protection against viral challenges even though it led to significant humoral immune responses similar to that induced by the peptide vaccine.

  19. An AO-assisted Variability Study of Four Globular Clusters

    Science.gov (United States)

    Salinas, R.; Contreras Ramos, R.; Strader, J.; Hakala, P.; Catelan, M.; Peacock, M. B.; Simunovic, M.

    2016-09-01

    The image-subtraction technique applied to study variable stars in globular clusters represented a leap in the number of new detections, with the drawback that many of these new light curves could not be transformed to magnitudes due to severe crowding. In this paper, we present observations of four Galactic globular clusters, M 2 (NGC 7089), M 10 (NGC 6254), M 80 (NGC 6093), and NGC 1261, taken with the ground-layer adaptive optics module at the SOAR Telescope, SAM. We show that the higher image quality provided by SAM allows for the calibration of the light curves of the great majority of the variables near the cores of these clusters as well as the detection of new variables, even in clusters where image-subtraction searches were already conducted. We report the discovery of 15 new variables in M 2 (12 RR Lyrae stars and 3 SX Phe stars), 12 new variables in M 10 (11 SX Phe and 1 long-period variable), and 1 new W UMa-type variable in NGC 1261. No new detections are found in M 80, but previous uncertain detections are confirmed and the corresponding light curves are calibrated into magnitudes. Additionally, based on the number of detected variables and new Hubble Space Telescope/UVIS photometry, we revisit a previous suggestion that M 80 may be the globular cluster with the richest population of blue stragglers in our Galaxy. Based on observations obtained at the Southern Astrophysical Research (SOAR) telescope, which is a joint project of the Ministério da Ciência, Tecnologia, e Inovação (MCTI) da República Federativa do Brasil, the U.S. National Optical Astronomy Observatory (NOAO), the University of North Carolina at Chapel Hill (UNC), and Michigan State University (MSU).

  20. The Age of the Inner Halo Globular Cluster NGC 6652

    OpenAIRE

    Chaboyer, Brian; Sarajedini, Ata; Armandroff, Taft E.

    2000-01-01

    HST (V,I) photometry has been obtained for the inner halo globular cluster NGC 6652. The photometry reaches approximately 4 mag below the turn-off and includes a well populated horizontal branch. This cluster is located close to the Galactic center at a galactocentric distance of approximately 2.0 kpc with a reddening of E(V-I) = 0.15 +/- 0.02 and has a metallicity of [Fe/H] approximately -0.85. Based upon Delta(V) between the point on the sub-giant branch which is 0.05 mag redder than the tu...

  1. The properties of the disk system of globular clusters

    Science.gov (United States)

    Armandroff, Taft E.

    1989-01-01

    A large refined data sample is used to study the properties and origin of the disk system of globular clusters. A scale height for the disk cluster system of 800-1500 pc is found which is consistent with scale-height determinations for samples of field stars identified with the Galactic thick disk. A rotational velocity of 193 + or - 29 km/s and a line-of-sight velocity dispersion of 59 + or - 14 km/s have been found for the metal-rich clusters.

  2. Properties of the disk system of globular clusters

    International Nuclear Information System (INIS)

    Armandroff, T.E.

    1989-01-01

    A large refined data sample is used to study the properties and origin of the disk system of globular clusters. A scale height for the disk cluster system of 800-1500 pc is found which is consistent with scale-height determinations for samples of field stars identified with the Galactic thick disk. A rotational velocity of 193 + or - 29 km/s and a line-of-sight velocity dispersion of 59 + or - 14 km/s have been found for the metal-rich clusters. 70 references

  3. The globular cluster ω Centauri and its RR Lyrae variables

    International Nuclear Information System (INIS)

    Dickens, R.J.

    1989-07-01

    The significance of some of the unusual characteristics of the globular cluster ωCentauri in various fundamental problems is explored. Interest is centred on the properties of the cluster RR Lyraes, and what they can contribute to studies of early cluster chemical enrichment, stellar pulsation, the distance scale, stellar evolution, stellar ages and the Oosterhoff period-shift problem. This article, which is intended to highlight problems and progress rather than give a comprehensive review, includes new results based on photometry of the RR Lyraes, red giants, subgiants, horizontal-branch and main sequence stars in the cluster. (author)

  4. Blue straggler stars in the globular cluster NGC 5053

    International Nuclear Information System (INIS)

    Nemec, J.M.; Cohen, J.G.

    1989-01-01

    A study of the low central concentration globular cluster NGC 5053 based on photometry to 23 mag is reported. Deep C-M diagrams are presented, a mean metal abundance for the cluster is derived from the color of the RGB at the level of the horizontal branch, and theoretical isochrones are used to derive a distance modulus of (m - M0) = 16.05 + or - 0.14 mag and an age of 18 + or - 3 Gyr. A luminosity function based on subgiant and upper main-sequence stars is also constructed. A total of 24 blue stragglers in NGC 5053 are identified and their properties are studied. 65 references

  5. The gravitational waveforms of white dwarf collisions in globular clusters

    International Nuclear Information System (INIS)

    Loren-Aguilar, P; Garcia-Berro, E; Lobo, J A; Isern, J

    2009-01-01

    In the dense central regions of globular clusters close encounters of two white dwarfs are relatively frequent. The estimated frequency is one or more strong encounters per star in the lifetime of the cluster. Such encounters should be then potential sources of gravitational wave radiation. Thus, it is foreseeable that these collisions could be either individually detected by LISA or they could contribute significantly to the background noise of the detector. We compute the pattern of gravitational wave emission from these encounters for a sufficiently broad range of system parameters, namely the masses, the relative velocities and the distances of the two white dwarfs involved in the encounter.

  6. A fast pulsar candidate in the globular cluster M28

    International Nuclear Information System (INIS)

    Mahoney, M.J.; Erickson, W.C.

    1985-01-01

    Recent work on radio sources in globular clusters, using the very large Array telescope at 1,465 MHz, revealed a source within the core of M28. Observations of this source at 30.9 and 57.5 MHz have also been carried out, by the authors, using the Clark lake TPT synthesis telescope. The observations show that the source has a spectral index of -2.44. Only pulsars have well-documented spectra which are as steep as this. (U.K.)

  7. Structure and Dynamics of the Globular Cluster Palomar 13

    Science.gov (United States)

    Bradford, J. D.; Geha, M.; Muñoz, R. R.; Santana, F. A.; Simon, J. D.; Côté, P.; Stetson, P. B.; Kirby, E.; Djorgovski, S. G.

    2011-12-01

    We present Keck/DEIMOS spectroscopy and Canada-France-Hawaii Telescope/MegaCam photometry for the Milky Way globular cluster Palomar 13. We triple the number of spectroscopically confirmed members, including many repeat velocity measurements. Palomar 13 is the only known globular cluster with possible evidence for dark matter, based on a Keck/High Resolution Echelle Spectrometer 21 star velocity dispersion of σ = 2.2 ± 0.4 km s-1. We reproduce this measurement, but demonstrate that it is inflated by unresolved binary stars. For our sample of 61 stars, the velocity dispersion is σ = 0.7+0.6 -0.5 km s-1. Combining our DEIMOS data with literature values, our final velocity dispersion is σ = 0.4+0.4 -0.3 km s-1. We determine a spectroscopic metallicity of [Fe/H] = -1.6 ± 0.1 dex, placing a 1σ upper limit of σ[Fe/H] ~ 0.2 dex on any internal metallicity spread. We determine Palomar 13's total luminosity to be MV = -2.8 ± 0.4, making it among the least luminous known globular clusters. The photometric isophotes are regular out to the half-light radius and mildly irregular outside this radius. The outer surface brightness profile slope is shallower than typical globular clusters (Σvpropr η, η = -2.8 ± 0.3). Thus at large radius, tidal debris is likely affecting the appearance of Palomar 13. Combining our luminosity with the intrinsic velocity dispersion, we find a dynamical mass of M 1/2 = 1.3+2: 7 -1.3 × 103 M ⊙ and a mass-to-light ratio of M/LV = 2.4+5.0 -2.4 M ⊙/L ⊙. Within our measurement errors, the mass-to-light ratio agrees with the theoretical predictions for a single stellar population. We conclude that, while there is some evidence for tidal stripping at large radius, the dynamical mass of Palomar 13 is consistent with its stellar mass and neither significant dark matter, nor extreme tidal heating, is required to explain the cluster dynamics. The data presented herein were obtained at the W. M. Keck Observatory, which is operated as a

  8. A spectroscopic study of chemical abundances in the globular cluster Omega Centauri

    International Nuclear Information System (INIS)

    Caldwell, S.P.

    1987-10-01

    Blue spectra at a resolution of 0.5 A of red giants in the globular clusters Omega Centauri and NGCs 288, 362, 6397 and 6809 (M55) have been obtained with the Anglo-Australian Telescope. The observations were made to test Sweigart and Mengel's [Astrophy S. J. 229, 624] theory of mixing of nuclearly-processed material to the star's surface, and to elucidate the relationship between primordial and evolutionary origins for the range in abundance within Omega Cen. The Omega Cen stars were chosen in two groups either side of the giant branch, covering the luminosity range where the onset of mixing was predicted to occur. Abundances of C, N, Fe and other heavy elements have been determined by fitting synthetic spectra, calculated from model atmospheres, to the observational data. (author)

  9. Discovery of a ~205 Hz X-ray pulsar in the globular cluster NGC 6440

    NARCIS (Netherlands)

    Altamirano, D.; Strohmayer, T.E.; Heinke, C.O.; Markwardt, C.B.; Swank, J.H.; Pereira, D.; Smith, E.; Wijnands, R.; Linares, M.; Patruno, A.; Casella, P.; van der Klis, M.

    2009-01-01

    Discovery of a 205 Hz X-ray pulsar in the globular cluster NGC 6440 The globular cluster NGC 6440 was observed by the PCA instrument aboard RXTE on August 30, 2009 at 01:42 (UTC). The observation lasted for approximately 3000 seconds and the source was detected with an intensity of ~7 mCrab (2-10

  10. A stochastic finite element model for the dynamics of globular macromolecules

    Science.gov (United States)

    Oliver, Robin C.; Read, Daniel J.; Harlen, Oliver G.; Harris, Sarah A.

    2013-04-01

    We describe a novel coarse-grained simulation method for modelling the dynamics of globular macromolecules, such as proteins. The macromolecule is treated as a continuum that is subject to thermal fluctuations. The model includes a non-linear treatment of elasticity and viscosity with thermal noise that is solved using finite element analysis. We have validated the method by demonstrating that the model provides average kinetic and potential energies that are in agreement with the classical equipartition theorem and that the nodal velocities have the correct Gaussian distribution. In addition, we have performed Fourier analysis on the simulation trajectories obtained for a series of linear beams to confirm that the correct average energies are present in the first two Fourier bending modes and that the probability distribution of the amplitudes of the first two Fourier modes match the theoretical results. We demonstrate spatial convergence of the model by showing that the anisotropy of the inertia tensor for a cubic mesh converges as a function of the mesh resolution. We have then used the new modelling method to simulate the thermal fluctuations of a representative protein over 500 ns timescales. Using reasonable parameters for the material properties, we have demonstrated that the overall deformation of the biomolecule is consistent with the results obtained for proteins in general from atomistic molecular dynamics simulations.

  11. On the evolution of globular clusters and the origin of galactic halo stars

    International Nuclear Information System (INIS)

    Surdin, V.G.

    1978-01-01

    Evolution of globular clusters of galactic halo is considered. It is shown that evolution of massive globular clusters with a greater degree of probability takes place under the effect of dynamic friction, which brings about the cluster fall on the center of galactic and their destruction by tidal forces. Evolution of small massive cluster takes place under the effect of dissipation. All the other reasons, causing the destruction of globular clusters (gravitational tidal forces, mutual cluster collision, outflow of gas from red gigant atmospheres, the change of the radius of the cluster orbit at the expense of the change of the galaxy mass inside the cluster orbit) play a secondary role. The whole mass of the stars lost by globular clusters does not exceed 10 7 M sun. It is concluded that the origin of the star population of galactic halo field can not be explained by destruction of already formed only astral globular clusters

  12. New VVV Survey Globular Cluster Candidates in the Milky Way Bulge

    Energy Technology Data Exchange (ETDEWEB)

    Minniti, Dante; Gómez, Matías [Departamento de Física, Facultad de Ciencias Exactas, Universidad Andrés Bello, Av. Fernandez Concha 700, Las Condes, Santiago (Chile); Geisler, Douglas; Fernández-Trincado, Jose G. [Departamento de Astronomía, Casilla 160-C, Universidad de Concepción, Casilla 160-C, Concepción (Chile); Alonso-García, Javier; Beamín, Juan Carlos; Borissova, Jura; Catelan, Marcio; Ramos, Rodrigo Contreras; Kurtev, Radostin; Pullen, Joyce [Instituto Milenio de Astrofísica, Santiago (Chile); Palma, Tali; Clariá, Juan J. [Observatorio Astronómico, Universidad Nacional de Córdoba, Laprida 854, Córdoba (Argentina); Cohen, Roger E. [Space Telescope Science Institute, 2700 San Martin Drive, Baltimore (United States); Dias, Bruno [European Southern Observatory, Alonso de Cordova 3107, Vitacura, Santiago (Chile); Hempel, Maren [Pontificia Universidad Católica de Chile, Instituto de Astrofísica, Av. Vicuña Mackenna 4860, Santiago (Chile); Ivanov, Valentin D. [European Southern Observatory, Karl-Schwarszchild-Str. 2, D-85748 Garching bei Muenchen (Germany); Lucas, Phillip W. [Dept. of Astronomy, University of Hertfordshire, Hertfordshire (United Kingdom); Moni-Bidin, Christian; Alegría, Sebastian Ramírez [Instituto de Astronomía, Universidad Católica del Norte, Antofagasta (Chile); and others

    2017-11-10

    It is likely that a number of Galactic globular clusters remain to be discovered, especially toward the Galactic bulge. High stellar density combined with high and differential interstellar reddening are the two major problems for finding globular clusters located toward the bulge. We use the deep near-IR photometry of the VISTA Variables in the Vía Láctea (VVV) Survey to search for globular clusters projected toward the Galactic bulge, and hereby report the discovery of 22 new candidate globular clusters. These objects, detected as high density regions in our maps of bulge red giants, are confirmed as globular cluster candidates by their color–magnitude diagrams. We provide their coordinates as well as their near-IR color–magnitude diagrams, from which some basic parameters are derived, such as reddenings and heliocentric distances. The color–magnitude diagrams reveal well defined red giant branches in all cases, often including a prominent red clump. The new globular cluster candidates exhibit a variety of extinctions (0.06 < A {sub Ks} < 2.77) and distances (5.3 < D < 9.5 kpc). We also classify the globular cluster candidates into 10 metal-poor and 12 metal-rich clusters, based on the comparison of their color–magnitude diagrams with those of known globular clusters also observed by the VVV Survey. Finally, we argue that the census for Galactic globular clusters still remains incomplete, and that many more candidate globular clusters (particularly the low luminosity ones) await to be found and studied in detail in the central regions of the Milky Way.

  13. SEARCH FOR RED DWARF STARS IN GLOBULAR CLUSTER NGC 6397

    Science.gov (United States)

    2002-01-01

    Left A NASA Hubble Space Telescope image of a small region (1.4 light-years across) in the globular star cluster NGC 6397. Simulated stars (diamonds) have been added to this view of the same region of the cluster to illustrate what astronomers would have expected to see if faint red dwarf stars were abundant in the Milky Way Galaxy. The field would then contain 500 stars, according to theoretical calculations. Right The unmodified HST image shows far fewer stars than would be expected, according to popular theories of star formation. HST resolves about 200 stars. The stellar density is so low that HST can literally see right through the cluster and resolve far more distant background galaxies. From this observation, scientists have identified the surprising cutoff point below which nature apparently doesn't make many stars smaller that 1/5 the mass of our Sun. These HST findings provide new insights into star formation in our Galaxy. Technical detail:The globular cluster NGC 6397, one of the nearest and densest agglomerations of stars, is located 7,200 light-years away in the southern constellation Ara. This visible-light picture was taken on March 3, 1994 with the Wide Field Planetary Camera 2, as part the HST parallel observing program. Credit: F. Paresce, ST ScI and ESA and NASA

  14. The Age of the Inner Halo Globular Cluster NGC 6652

    Science.gov (United States)

    Chaboyer, Brian; Sarajedini, Ata; Armandroff, Taft E.

    2000-01-01

    Hubble Space Telescope (HST) (V,I) photometry has been obtained for the inner halo globular cluster NGC 6652. The photometry reaches approximately 4 mag below the turn-off and includes a well populated horizontal branch (HB). This cluster is located close to the Galactic center at RGC approximately equal to 2.0 kpc with a reddening of E(V-I) = 0.15 +/- 0.02 and has a metallicity of [Fe/H] approximately equal to -0.85. Based upon DELTA V (sup SGB) (sub HB), NGC 6652 is 11.7 plus or minus 1.6 Gyr old. Using A HB precise differential ages for 47 Tuc (a thick disk globular), M107 and NGC 1851 (both halo clusters) were obtained. NGC 6652 appears to be the same age as 47 Tuc and NGC 1851 (within +/- 1.2 Gyr), while there is a slight suggestion that M107 is older than NGC 6652 by 2.3 +/- 1.5 Gyr. As this is a less than 2 sigma result, this issue needs to be investigated further before a definitive statement regarding the relative age of M107 and NGC 6652 may be made.

  15. An Archival Search For Young Globular Clusters in Galaxies

    Science.gov (United States)

    Whitmore, Brad

    1995-07-01

    One of the most intriguing results from HST has been the discovery of ultraluminous star clusters in interacting and merging galaxies. These clusters have the luminosities, colors, and sizes that would be expected of young globular clusters produced by the interaction. We propose to use the data in the HST Archive to determine how prevalent this phenomena is, and to determine whether similar clusters are produced in other environments. Three samples will be extracted and studied in a systematic and consistent manner: 1} interacting and merging galaxies, 2} starburst galaxies, 3} a control sample of ``normal'' galaxies. A preliminary search of the archives shows that there are at least 20 galaxies in each of these samples, and the number will grow by about 50 observations become available. The data will be used to determine the luminosity function, color histogram , spatial distribution, and structural properties of the clusters using the same techniques employed in our study of NGC 7252 {``Atoms -for-Peace'' galaxy} and NGC 4038/4039 {``The Antennae''}. Our ultimate goals are: 1} to understand how globular clusters form, and 2} to use the clusters as evolutionary tracers to unravel the histories of interacting galaxies.

  16. Deep CCD photometry in globular clusters III. M15

    International Nuclear Information System (INIS)

    Fahlman, G.G.; Richer, H.B.; Vandenberg, D.A.

    1985-01-01

    CCD photometry in U, B, and V is presented for a 5' x 3' field in the globular cluster M15. The location of the main sequence in the color-magnitude diagram is found here to be significantly bluer than previous studies have indicated. The luminosity function of the cluster is studied down to V = 22.8 (Mroughly-equal7.5) and shown to be consistent with a power-law mass function, n(M) = QM/sup -alpha/ with α = 2.5 +- 1.0, to the limit of our data. The field star population brighter than V = 21.5, is examined in some detail. There appears to be about 50% more stars belonging to the disk in the field as compared with the Bahcall-Soneira standard galaxy model. The reddening to the cluster is found to be E(B-V) = 0.11 +- 0.04 from nine bright field stars. A new value for the ultraviolet excess of the cluster main-sequence stars is obtained, delta(0.6) = 0.25 +- 0.02, and confirms the well-known fact that M15 is among the metal poorest of the globular clusters

  17. Brain asymmetry in the white matter making and globularity

    Directory of Open Access Journals (Sweden)

    Constantina eTheofanopoulou

    2015-09-01

    Full Text Available Recent studies from the field of language genetics and evolutionary anthropology have put forward the hypothesis that the emergence of our species-specific brain is to be understood not in terms of size, but in light of developmental changes that gave rise to a more globular braincase configuration after the split from Neanderthals-Denisovans. On the grounds that (i white matter myelination is delayed relative to other brain structures and in humans is protracted compared with other primates and (ii neural connectivity is linked genetically to our brain/skull morphology and language-ready brain, I take it that one significant evolutionary change in Homo sapiens’ lineage is the interhemispheric connectivity mediated by the Corpus Callosum. The size, myelination and fiber caliber of the Corpus Callosum presents an anterior-to-posterior increase, in a way that inter-hemispheric connectivity is more prominent in the sensory motor areas, whereas high- order areas are more intra-hemispherically connected. Building on evidence from language-processing studies that account for this asymmetry (‘lateralization’ in terms of brain rhythms, I present an evo-devo hypothesis according to which the myelination of the Corpus Callosum, Brain Asymmetry and Globularity are conjectured to make up the angles of a co-evolutionary triangle that gave rise to our language-ready brain.

  18. Medium Resolution Spectroscopy and Chemical Composition of Galactic Globular Clusters

    Directory of Open Access Journals (Sweden)

    Khamidullina D. A.

    2014-12-01

    Full Text Available We used integrated-light medium-resolution spectra of six Galactic globular clusters and model stellar atmospheres to carry out population synthesis and to derive chemical composition and age of the clusters. We used medium-resolution spectra of globular clusters published by Schiavon et al. (2005, as well as our long-slit observations with the 1.93 m telescope of the Haute Provence Observatory. The observed spectra were fitted to the theoretical ones interactively. As an initial approach, we used masses, radii and log g of stars in the clusters corresponding to the best fitting isochrones in the observed color-magnitude diagrams. The computed synthetic blanketed spectra of stars were summed according to the Chabrier mass function. To improve the determination of age and helium content, the shape and depth of the Balmer absorption lines was analysed. The abundances of Mg, Ca, C and several other elements were derived. A reasonable agreement with the literature data both in chemical composition and in age of the clusters is found. Our method might be useful for the development of stellar population models and for a better understanding of extragalactic star clusters.

  19. The fragmentation of proto-globular clusters. I. Thermal instabilities

    International Nuclear Information System (INIS)

    Murray, S.D.; Lin, D.N.C.

    1989-01-01

    The metal abundances among the stars within a typical globular cluster are remarkably homogeneous. This indicates that star formation in these systems was a globally coordinated event which occurred over a time span less than or comparable to the collapse time scale of the cluster. This issue is addressed by assuming that the fragmentation of a proto-globular cluster cloud proceeded in two steps. In the first step, thermal instability led to the rapid growth of initial fluctuations. This led to a large contrast in the dynamical time scales between the perturbations and the parent cloud, and the perturbations then underwent gravitational instabilities on short time scales. This process is modeled using one-dimensional hydrodynamic simulations of clouds both with and without external heat sources and self-gravity. The models include the effects of a non-equilibrium H2 abundance. The results indicate that fragmentation can occur on time scales significantly less than the dynamical time scale of the parent cloud. 21 refs

  20. Comparing Chemical Compositions of Dwarf Elliptical Galaxies and Globular Clusters

    Science.gov (United States)

    Chu, Jason; Sparkman, Lea; Toloba, Elisa; Guhathakurta, Puragra

    2015-01-01

    Because of their abundance in cluster environments and fragility due to their low mass, dwarf elliptical galaxies (dEs) are excellent specimens for studying the physical processes that occur inside galaxy clusters. These studies can be used to expand our understanding of the process of galaxy (specifically dE) formation and the role of dark matter in the Universe. To move closer to better understanding these topics, we present a study of the relationship between dEs and globular clusters (GCs) by using the largest sample of dEs and GC satellites to date. We focus on comparing the ages and chemical compositions of dE nuclei with those of satellite GCs by analyzing absorption lines in their spectra. To better view the spectral features of these relatively dim objects, we employ a spectral co-addition process, where we add the fluxes of several objects to produce a single spectrum with high signal-to-noise ratio. Our finding that dE nuclei are younger and more metal rich than globular clusters establishes important benchmarks that future dE formation theories will consider. We also establish a means to identify GCs whose parent galaxies are uncertain, which allows us to make comparisons between this GC group and the satellite GCs.

  1. Variable Stars in Large Magellanic Cloud Globular Clusters. III. Reticulum

    Science.gov (United States)

    Kuehn, Charles A.; Dame, Kyra; Smith, Horace A.; Catelan, Márcio; Jeon, Young-Beom; Nemec, James M.; Walker, Alistair R.; Kunder, Andrea; Pritzl, Barton J.; De Lee, Nathan; Borissova, Jura

    2013-06-01

    This is the third in a series of papers studying the variable stars in old globular clusters in the Large Magellanic Cloud. The primary goal of this series is to look at how the characteristics and behavior of RR Lyrae stars in Oosterhoff-intermediate systems compare to those of their counterparts in Oosterhoff-I/II systems. In this paper we present the results of our new time-series BVI photometric study of the globular cluster Reticulum. We found a total of 32 variables stars (22 RRab, 4 RRc, and 6 RRd stars) in our field of view. We present photometric parameters and light curves for these stars. We also present physical properties, derived from Fourier analysis of light curves, for some of the RR Lyrae stars. We discuss the Oosterhoff classification of Reticulum and use our results to re-derive the distance modulus and age of the cluster. Based on observations taken with the SMARTS 1.3 m telescope operated by the SMARTS Consortium and observations taken at the Southern Astrophysical Research (SOAR) telescope, which is a joint project of the Ministério da Ciência, Tecnologia, e Inovação (MCTI) da República Federativa do Brasil, the U.S. National Optical Astronomy Observatory (NOAO), the University of North Carolina at Chapel Hill (UNC), and Michigan State University (MSU).

  2. Color Gradients Within Globular Clusters: Restricted Numerical Simulation

    Directory of Open Access Journals (Sweden)

    Young-Jong Sohn

    1997-06-01

    Full Text Available The results of a restricted numerical simulation for the color gradients within globular clusters have been presented. The standard luminosity function of M3 and Salpeter's initial mass functions were used to generate model clusters as a fundamental population. Color gradients with the sample clusters for both King and power law cusp models of surface brightness distributions are discussed in the case of using the standard luminosity function. The dependence of color gradients on several parameters for the simulations with Salpeter's initial mass functions, such as slope of initial mass functions, cluster ages, metallicities, concentration parameters of King model, and slopes of power law, are also discussed. No significant radial color gradients are shown to the sample clusters which are regenerated by a random number generation technique with various parameters in both of King and power law cusp models of surface brightness distributions. Dynamical mass segregation and stellar evolution of horizontal branch stars and blue stragglers should be included for the general case of model simulations to show the observed radial color gradients within globular clusters.

  3. Medium resolution spectroscopy and chemical composition of Galactic globular clusters

    Science.gov (United States)

    Khamidullina, D. A.; Sharina, M. E.; Shimansky, V. V.; Davoust, E.

    We used integrated-light medium-resolution spectra of six Galactic globular clusters and model stellar atmospheres to carry out population synthesis and to derive chemical composition and age of the clusters. We used medium-resolution spectra of globular clusters published by Schiavon et al. (2005), as well as our long-slit observations with the 1.93 m telescope of the Haute Provence Observatory. The observed spectra were fitted to the theoretical ones interactively. As an initial approach, we used masses, radii and log g of stars in the clusters corresponding to the best fitting isochrones in the observed color-magnitude diagrams. The computed synthetic blanketed spectra of stars were summed according to the Chabrier mass function. To improve the determination of age and helium content, the shape and depth of the Balmer absorption lines was analysed. The abundances of Mg, Ca, C and several other elements were derived. A reasonable agreement with the literature data both in chemical composition and in age of the clusters is found. Our method might be useful for the development of stellar population models and for a better understanding of extragalactic star clusters.

  4. Ages of Globular Clusters from HIPPARCOS Parallaxes of Local Subdwarfs

    Science.gov (United States)

    Gratton, Raffaele G.; Fusi Pecci, Flavio; Carretta, Eugenio; Clementini, Gisella; Corsi, Carlo E.; Lattanzi, Mario

    1997-12-01

    We report here initial but strongly conclusive results for absolute ages of Galactic globular clusters (GGCs). This study is based on high-precision trigonometric parallaxes from the HIPPARCOS satellite coupled with accurate metal abundances ([Fe/H], [O/Fe], and [α/Fe]) from high-resolution spectroscopy for a sample of about thirty subdwarfs. Systematic effects due to star selection (Lutz-Kelker corrections to parallaxes) and the possible presence of undetected binaries in the sample of bona fide single stars are examined, and appropriate corrections are estimated. They are found to be small for our sample. The new data allow us to reliably define the absolute location of the main sequence (MS) as a function of metallicity. These results are then used to derive distances and ages for a carefully selected sample of nine globular clusters having metallicities determined from high-dispersion spectra of individual giants according to a procedure totally consistent with that used for the field subdwarfs. Very precise and homogeneous reddening values have also been independently determined for these clusters. Random errors for our distance moduli are +/-0.08 mag, and systematic errors are likely of the same order of magnitude. These very accurate distances allow us to derive ages with internal errors of ~12% (+/-1.5 Gyr). The main results are: 1. HIPPARCOS parallaxes are smaller than corresponding ground-based measurements, leading, in turn, to longer distance moduli (~0.2 mag) and younger ages (~2.8 Gyr). 2. The distance to NGC 6752 derived from our MS fitting is consistent with that determined using the white dwarf cooling sequence. 3. The relation between the zero-age HB (ZAHB) absolute magnitude and metallicity for the nine program clusters is MV(ZAHB)=(0.22+/-0.09)([Fe/H]+1.5)+(0.49+/-0.04) . This relation is fairly consistent with some of the most recent theoretical models. Within quoted errors, the slope is in agreement with that given by the Baade-Wesselink (BW

  5. MONTE CARLO SIMULATIONS OF GLOBULAR CLUSTER EVOLUTION. V. BINARY STELLAR EVOLUTION

    International Nuclear Information System (INIS)

    Chatterjee, Sourav; Umbreit, Stefan; Rasio, Frederic A.; Fregeau, John M.

    2010-01-01

    We study the dynamical evolution of globular clusters containing primordial binaries, including full single and binary stellar evolution using our Monte Carlo cluster evolution code updated with an adaptation of the single and binary stellar evolution codes SSE and BSE from Hurley et al. We describe the modifications that we have made to the code. We present several test calculations and comparisons with existing studies to illustrate the validity of the code. We show that our code finds very good agreement with direct N-body simulations including primordial binaries and stellar evolution. We find significant differences in the evolution of the global properties of the simulated clusters using stellar evolution compared with simulations without any stellar evolution. In particular, we find that the mass loss from the stellar evolution acts as a significant energy production channel simply by reducing the total gravitational binding energy and can significantly prolong the initial core contraction phase before reaching the binary-burning quasi-steady state of the cluster evolution. We simulate a large grid of models varying the initial cluster mass, binary fraction, and concentration parameter, and we compare properties of the simulated clusters with those of the observed Galactic globular clusters (GGCs). We find that simply including stellar evolution in our simulations and assuming the typical initial cluster half-mass radius is approximately a few pc independent of mass, our simulated cluster properties agree well with the observed GGC properties such as the core radius and the ratio of the core radius to the half-mass radius. We explore in some detail qualitatively different clusters in different phases of their evolution and construct synthetic Hertzsprung-Russell diagrams for these clusters.

  6. Statistical method for determining ages of globular clusters by fitting isochrones

    International Nuclear Information System (INIS)

    Flannery, B.P.; Johnson, B.C.

    1982-01-01

    We describe a statistical procedure to compare models of stellar evolution and atmospheres with color-magnitude diagrams of globular clusters. The isochrone depends on five parameters: m-M, age, [Fe/H], Y, and α, but in practice we can only determine m-M and age for an assumed composition. The technique allows us to determine parameters of the model, their uncertainty, and to assess goodness of fit. We test the method, and evaluate the effect of assumptions on an extensive set of Monte Carlo simulations. We apply the method to extensive observations of NGC 6752 and M5, and to smaller data sets for the clusters M3, M5, M15, and M92. We determine age and m-M for two assumed values of helium Y = (0.2, 0.3), and three values of metallicity with a spread in [Fe/H] of +- 0.3 dex. These result in a spread in age of 5-8 Gyr (1 Gyr = 10 9 yr), and a spread in m-M of 0.5 mag. The mean age is generally younger by 2-3 Gyr than previous estimates. Likely uncertainty associated with an individual fit can be small as 0.4 Gyr. Most importantly, we find that two uncalibratable sources of systematic error make the results suspect. These are uncertainty in the stellar temperatures induced by choice of mixing length, and known errors in stellar atmospheres. These effects could reduce age estimates by an additional 5 Gyr. We conclude that observations do not preclude ages as young as 10 Gyr for globular clusters

  7. FURTHER DEFINITION OF THE MASS-METALLICITY RELATION IN GLOBULAR CLUSTER SYSTEMS AROUND BRIGHTEST CLUSTER GALAXIES

    International Nuclear Information System (INIS)

    Cockcroft, Robert; Harris, William E.; Wehner, Elizabeth M. H.; Whitmore, Bradley C.; Rothberg, Barry

    2009-01-01

    We combine the globular cluster (GC) data for 15 brightest cluster galaxies and use this material to trace the mass-metallicity relations (MMRs) in their globular cluster systems (GCSs). This work extends previous studies which correlate the properties of the MMR with those of the host galaxy. Our combined data sets show a mean trend for the metal-poor subpopulation that corresponds to a scaling of heavy-element abundance with cluster mass Z ∼ M 0.30±0.05 . No trend is seen for the metal-rich subpopulation which has a scaling relation that is consistent with zero. We also find that the scaling exponent is independent of the GCS specific frequency and host galaxy luminosity, except perhaps for dwarf galaxies. We present new photometry in (g',i') obtained with Gemini/GMOS for the GC populations around the southern giant ellipticals NGC 5193 and IC 4329. Both galaxies have rich cluster populations which show up as normal, bimodal sequences in the color-magnitude diagram. We test the observed MMRs and argue that they are statistically real, and not an artifact caused by the method we used. We also argue against asymmetric contamination causing the observed MMR as our mean results are no different from other contamination-free studies. Finally, we compare our method to the standard bimodal fitting method (KMM or RMIX) and find our results are consistent. Interpretation of these results is consistent with recent models for GC formation in which the MMR is determined by GC self-enrichment during their brief formation period.

  8. THE HUBBLE SPACE TELESCOPE UV LEGACY SURVEY OF GALACTIC GLOBULAR CLUSTERS. VIII. PRELIMINARY PUBLIC CATALOG RELEASE

    Energy Technology Data Exchange (ETDEWEB)

    Soto, M.; Bellini, A.; Anderson, J.; Van der Marel, R. P.; Brown, T. M. [Space Telescope Science Institute, San Martin Drive 3700, Baltimore, MD 21218 (United States); Piotto, G.; Granata, V.; Ortolani, S.; Nardiello, D. [Dipartimento di Fisica e Astronomia Galileo Galilei, Università di Padova, Vicolo dell’Osservatorio 3, I-35122 Padova (Italy); Bedin, L. R. [INAF-Osservatorio Astronomico di Padova, Vicolo dell’Osservatorio 5, I-35122 Padova (Italy); Milone, A. P. [Research School of Astronomy and Astrophysics, The Australian National University, Cotter Road, Weston, ACT, 2611 (Australia); Cool, A. M. [Department of Physics and Astronomy, San Francisco State University, 1600 Holloway Avenue, San Francisco, CA 94132 (United States); King, I. R. [Department of Astronomy, University of Washington, Box 351580, Seattle, WA 98195-1580 (United States); Sarajedini, A. [Department of Astronomy, University of Florida, 211 Bryant Space Science Center, Gainesville, FL 32611 (United States); Cassisi, S. [Osservatorio Astronomico di Teramo, Via Mentore Maggini s.n.c., I-64100 Teramo (Italy); Aparicio, A.; Hidalgo, S., E-mail: mario.soto@uda.cl [Instituto de Astrofísica de Canarias, E-38200 La Laguna, Tenerife, Canary Islands (Spain)

    2017-01-01

    The Hubble Space Telescope (HST) UV Legacy Survey of Galactic Globular Clusters (GO-13297) has been specifically designed to complement the existing F606W and F814W observations of the Advanced Camera for Surveys (ACS) Globular Cluster Survey (GO-10775) by observing the most accessible 47 of the previous survey’s 65 clusters in three WFC3/UVIS filters F275W, F336W, and F438W. The new survey also adds super-solar metallicity open cluster NGC 6791 to increase the metallicity diversity. The combined survey provides a homogeneous 5-band data set that can be used to pursue a broad range of scientific investigations. In particular, the chosen UV filters allow the identification of multiple stellar populations by targeting the regions of the spectrum that are sensitive to abundance variations in C, N, and O. In order to provide the community with uniform preliminary catalogs, we have devised an automated procedure that performs high-quality photometry on the new UV observations (along with similar observations of seven other programs in the archive). This procedure finds and measures the potential sources on each individual exposure using library point-spread functions and cross-correlates these observations with the original ACS-Survey catalog. The catalog of 57 clusters we publish here will be useful to identify stars in the different stellar populations, in particular for spectroscopic follow-up. Eventually, we will construct a more sophisticated catalog and artificial-star tests based on an optimal reduction of the UV survey data, but the catalogs presented here give the community the chance to make early use of this HST Treasury survey.

  9. Determination of immune status in dogs against CPV-2 by recombinant protein based latex agglutination test.

    Science.gov (United States)

    Thomas, Jobin; Singh, Mithilesh; Goswami, T K; Glora, Philma; Chakravarti, Soumendu; Chander, Vishal; Upmanyu, Vikramaditya; Verma, Suman; Sharma, Chhavi; Mahendran, K

    2017-09-01

    Canine parvoviral enteritis is a highly contagious viral illness caused by canine parvovirus-2 (CPV-2) which affects puppies of mainly 6-20 weeks of age. Vaccination is pivotal in preventing and controlling CPV-2 infection. Determination of antibody status is a critical determinant for successful vaccination. The hemagglutination inhibition (HI) test is 'gold standard' test for quantification of antibodies specific to CPV-2, although the execution of this test is not feasible under field conditions. The present study was undertaken to develop a point of care testing to determine immune status prior to CPV-2 vaccination or to detect seroconversion in immunized dogs by latex agglutination test (LAT) using recombinant antigen. Truncated portion of VP2 protein (tVP2) of CPV-2 was selected on the basis of antigenic indices, overexpressed the recombinant protein in E. coli system and was subsequently used in development of LAT. A total of 59 serum samples obtained from vaccinated (n = 54) and healthy unvaccinated (n = 5) dogs were tested. The positivity was observed in 85% (46/54) of these dogs with varying agglutination pattern. The overall sensitivity and specificity of latex agglutination test in comparison to HI test was recorded as 90% and 88% respectively with an agreement value of 90% (CI = 95%). Copyright © 2017 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.

  10. Intraperitoneal administration of the globular adiponectin gene ameliorates diabetic nephropathy in Wistar rats.

    Science.gov (United States)

    Yuan, Fang; Liu, Ying-Hong; Liu, Fu-You; Peng, You-Ming; Tian, Jun-Wei

    2014-06-01

    The present study investigated the potential effects of the long-term expression of exogenous adiponectin (ADPN) on normal and diabetic kidneys. Type 2 diabetes mellitus models were induced by high-lipid and high-sucrose feeding plus intraperitoneal injection of streptozotocin. The recombinant plasmid pIRES2-EGFP-gAd, which is able to co-express globular ADPN (gAd) and enhanced green fluorescent protein (EGFP), was intraperitoneally injected into rat models mediated by Lipofectamine. In total, 32 Wistar rats were randomly assigned into four groups: the normal control group, the diabetes group, the diabetes group treated with pIRES2-EGFP-gAd and the diabetes group treated with pIRES2-EGFP. After 12 weeks, serum biochemistry and urine albumin levels were measured. The kidneys were collected to assess the generation of reactive oxygen species (ROS) and the renal pathological changes were observed by light microcopy. The protein expression of endothelial nitric oxide synthase (eNOS), transforming growth factor-β1 (TGF-β1) and phosphorylated adenosine monophosphate-activated protein kinase (p-AMPK) were determined by an immunohistochemical staining method and western blot analysis. Intraperitoneal injection of the human gAd gene via Lipofectamine resulted in abundant ADPN protein in the kidney. In the diabetic rats, the delivery of the exogenous gAd gene ameliorated the progression of diabetic nephropathy (DN). ADPN attenuated urine albumin excretion in the diabetic rats. ADPN also mitigated glomerular mesangial expansion, reduced the generation of ROS and prevented interstitial fibrosis. In addition, the expression of gAd inhibited the renal expression of TGF-β1, promoted the protein expression of eNOS and activated the opening of the AMPK signaling pathway in the renal tissues of the diabetic rats. Despite the effects of ADPN on DN being controversial, these observations indicate that the supplementation of ADPN is beneficial in ameliorating DN in rats.

  11. High dose brain CT with ioxaglate and diatrizoate adverse reactions and effects on urine protein tests

    International Nuclear Information System (INIS)

    Raininko, R.; Laivola, J.; Irjala, K.; Turku Univ. Central Hospital

    1988-01-01

    Sodium meglumine ioxaglate (320 or 306 mg I/ml) and meglumine diatrizoate (306 mg I/ml) in an intravenous dose of 2 ml/kg were compared in a randomized double-blind test on the brain CT of 209 patients. Side effects were noted in 56% of the ioxaglate group and 90% of the diatrizoate group. Diatrizoate caused a sensation of heat significantly more often and more intensely, but the frequencies of other side effects did not differ significantly. No severe reactions occurred. The quality of the CT scans was equal. Neither ioxaglate nor diatrizoate impaired renal function. False-positive strip tests and falsely elevated protein values measured by the biuretic method were found in particular in the ioxaglate group. The results of urine protein measurements and strip tests are misleading on the day of the examination with both ioxaglate and diatrizoate. (orig.)

  12. Testing of gastric contents for peanut proteins in a 13-year old anaphylaxis victim.

    Science.gov (United States)

    Beavers, Charles; Stauble, M Elaine; Jortani, Saeed A

    2014-02-15

    We report the case of a 13-y female who went into anaphylactic shock following the ingestion of a meal suspected to be contaminated by peanuts. The teenager had a known sensitivity to peanuts, however, the restaurant claimed that no peanut products were used in the preparation of her meal. The gastric contents of the decedent were retained and tested for peanut proteins due to the possible legal liability of the proprietor. Using antibodies against peanut proteins (roasted and unroasted), we optimized a method to detect total soluble peanut proteins by Western-blot analysis in gastric contents. In addition, we validated two commercially available tests which were originally intended for detection of peanut proteins in food matrices to examine the same gastric sample. One was an enzyme-linked immunosorbent assay (ELISA) that utilized polyclonal antibodies against Ara h 1 (Tepnel Life Sciences). The other was a laminar-flow assay directed against Ara h 1, Ara h 2 and Ara h 3 (R-Biopharm). A positive food-based control was created by reducing bread and peanuts (1:1, w/w) with water (1:1, w/v) using a mortar and pestle. A food-based negative food control was created similar to the positive control, except the peanuts were omitted and the amount of bread was doubled. The Western-blot assay was sensitive down to 2.5ng/ml of total peanut protein. The laminar flow was the most rapid and least complex. The ELISA was the most analytically sensitive with a cut-off of 1ng/ml of Ara h 1 protein compared to the laminar flow which had a cut-off of 4ng/ml Ara h 1 equivalent. Both ELISA and laminar flow assays were able to detect peanut proteins in the food matrices and positive controls, and not in negative controls. No peanut related proteins were detected in the decedent's gastric sample. The gastric sample spiked with peanuts was reliably detectable. The anaphylaxis patient had no peanut allergens detected in her gastric contents by any of the three methods employed. Both

  13. The gamma-ray pulsar population of globular clusters: implications for the GeV excess

    Energy Technology Data Exchange (ETDEWEB)

    Hooper, Dan [Fermi National Accelerator Laboratory, Center for Particle Astrophysics, Batavia, IL 60510 (United States); Linden, Tim, E-mail: dhooper@fnal.gov, E-mail: linden.70@osu.edu [Ohio State University, Center for Cosmology and AstroParticle Physcis (CCAPP), Columbus, OH 43210 (United States)

    2016-08-01

    It has been suggested that the GeV excess, observed from the region surrounding the Galactic Center, might originate from a population of millisecond pulsars that formed in globular clusters. With this in mind, we employ the publicly available Fermi data to study the gamma-ray emission from 157 globular clusters, identifying a statistically significant signal from 25 of these sources (ten of which are not found in existing gamma-ray catalogs). We combine these observations with the predicted pulsar formation rate based on the stellar encounter rate of each globular cluster to constrain the gamma-ray luminosity function of millisecond pulsars in the Milky Way's globular cluster system. We find that this pulsar population exhibits a luminosity function that is quite similar to those millisecond pulsars observed in the field of the Milky Way (i.e. the thick disk). After pulsars are expelled from a globular cluster, however, they continue to lose rotational kinetic energy and become less luminous, causing their luminosity function to depart from the steady-state distribution. Using this luminosity function and a model for the globular cluster disruption rate, we show that millisecond pulsars born in globular clusters can account for only a few percent or less of the observed GeV excess. Among other challenges, scenarios in which the entire GeV excess is generated from such pulsars are in conflict with the observed mass of the Milky Way's Central Stellar Cluster.

  14. A catalogue of masses, structural parameters and velocity dispersion profiles of 112 Milky Way globular clusters

    Science.gov (United States)

    Baumgardt, H.; Hilker, M.

    2018-05-01

    We have determined masses, stellar mass functions and structural parameters of 112 Milky Way globular clusters by fitting a large set of N-body simulations to their velocity dispersion and surface density profiles. The velocity dispersion profiles were calculated based on a combination of more than 15,000 high-precision radial velocities which we derived from archival ESO/VLT and Keck spectra together with ˜20, 000 published radial velocities from the literature. Our fits also include the stellar mass functions of the globular clusters, which are available for 47 clusters in our sample, allowing us to self-consistently take the effects of mass segregation and ongoing cluster dissolution into account. We confirm the strong correlation between the global mass functions of globular clusters and their relaxation times recently found by Sollima & Baumgardt (2017). We also find a correlation of the escape velocity from the centre of a globular cluster and the fraction of first generation stars (FG) in the cluster recently derived for 57 globular clusters by Milone et al. (2017), but no correlation between the FG star fraction and the global mass function of a globular cluster. This could indicate that the ability of a globular cluster to keep the wind ejecta from the polluting star(s) is the crucial parameter determining the presence and fraction of second generation stars and not its later dynamical mass loss.

  15. Profile of Students' Creative Thinking Skills on Quantitative Project-Based Protein Testing using Local Materials

    Directory of Open Access Journals (Sweden)

    D. K. Sari

    2017-04-01

    Full Text Available The purpose of this study is to obtain a profile of students’ creative thinking skills on quantitative project-based protein testing using local materials. Implementation of the research is using quasi-experimental method pre-test post-test control group design with 40 students involved in Biochemistry lab. The research instrument is pre-test and post-test using creative thinking skills in the form of description and students’ questionnaire. The analysis was performed with SPSS 22.0 program to see the significance normality, U Mann-Whitney test for nonparametric statistics, N-Gain score, and the percentage of student responses to the practicum performed. The research result shows that the pretest rate in the experimental group is 8.25 while in the control group is 6.90. After attending a project-based practicum with local materials, the experimental group obtained the mean of posttest is 37.55 while in control class is 11.18. The students’ improvement on creative thinking skills can be seen from the average of N-Gain in the experimental class with 0.32 (medium category and in the control category with 0.05 (low category. The experimental and control class have different creative thinking skills significantly different fluency, flexibility, novelty, and detail. It can be concluded that quantitative project-based protein testing using local materials can improve students’ creative thinking skills. 71% of total students feel that quantitative project-based protein testing using local materials make them more creative in doing a practicum in the laboratory.

  16. Cyanogen strengths of globular cluster post-main-sequence stars

    International Nuclear Information System (INIS)

    Hesser, J.E.; Hartwick, F.D.A.; McClure, R.D.

    1976-01-01

    CN strengths in the peculiar clusters ω Cen and M22 and the metal-rich clusters 47 Tuc, M71, and NGC 6352 are found to vary markedly from star to star. The strong variations in CN strength found earlier for ω Cen by Norris and Bessell and by Dickens and Bell are shown to extend to fainter stars, although expected correlations of CN strength with position in the color-magnitude (C-M) diagram are less evident in our sample. Several CN and metal-strong stars were also observed in M22. We conclude that CN, once it appears in globular clusters, can vary much more than it does in equivalent Population I samples, a result we briefly examine in light of current understanding regarding physical processes in the stars themselves and of models of galactic chemical evolution

  17. Chemical abundances of globular clusters in NGC 5128 (Centaurus A)

    Science.gov (United States)

    Hernandez, Svea; Larsen, Søren; Trager, Scott; Kaper, Lex; Groot, Paul

    2018-06-01

    We perform a detailed abundance analysis on integrated-light spectra of 20 globular clusters (GCs) in the early-type galaxy NGC 5128 (Centaurus A). The GCs were observed with X-Shooter on the Very Large Telescope (VLT). The cluster sample spans a metallicity range of -1.92 poor GCs in NGC 5128 is genuine, it could hint at a chemical enrichment history different than that experienced by the MW. We also measure Na abundances in 9 out of 20 GCs. We find evidence for intracluster abundance variations in six of these clusters where we see enhanced [Na/Fe] > +0.25 dex. We obtain the first abundance measurements of Cr, Mn, and Ni for a sample of the GC population in NGC 5128 and find consistency with the overall trends observed in the MW, with a slight enhancement (<0.1 dex) in the Fe-peak abundances measured in the NGC 5128.

  18. LISA Sources in Milky Way Globular Clusters.

    Science.gov (United States)

    Kremer, Kyle; Chatterjee, Sourav; Breivik, Katelyn; Rodriguez, Carl L; Larson, Shane L; Rasio, Frederic A

    2018-05-11

    We explore the formation of double-compact-object binaries in Milky Way (MW) globular clusters (GCs) that may be detectable by the Laser Interferometer Space Antenna (LISA). We use a set of 137 fully evolved GC models that, overall, effectively match the properties of the observed GCs in the MW. We estimate that, in total, the MW GCs contain ∼21 sources that will be detectable by LISA. These detectable sources contain all combinations of black hole (BH), neutron star, and white dwarf components. We predict ∼7 of these sources will be BH-BH binaries. Furthermore, we show that some of these BH-BH binaries can have signal-to-noise ratios large enough to be detectable at the distance of the Andromeda galaxy or even the Virgo cluster.

  19. SHRINKING THE BRANEWORLD: BLACK HOLE IN A GLOBULAR CLUSTER

    International Nuclear Information System (INIS)

    Gnedin, Oleg Y.; Maccarone, Thomas J.; Psaltis, Dimitrios; Zepf, Stephen E.

    2009-01-01

    Large extra dimensions have been proposed as a possible solution to the hierarchy problem in physics. In one of the suggested models, the RS2 braneworld model, black holes may evaporate by Hawking radiation faster than in general relativity, on a timescale that depends on the black hole mass and on the asymptotic radius of curvature of the extra dimensions. Thus the size of the extra dimensions can be constrained by astrophysical observations. Here we point out that the black hole, recently discovered in an extragalactic globular cluster, places the strongest upper limit on the size of the extra dimensions in the RS2 model, L ∼< 0.003 mm. This black hole has the virtues of old age and relatively small mass. The derived upper limit is within an order of magnitude of the absolute limit afforded by astrophysical observations of black holes.

  20. Search for Formation Criteria for Globular Cluster Systems

    Science.gov (United States)

    Nuritdinov, S. N.; Mirtadjieva, K. T.; Tadjibaev, I. U.

    2005-01-01

    Star cluster formation is a major mode of star formation in the extreme conditions of interacting galaxies and violent starbursts. By studying ages and metallicities of young metal-enhanced star clusters in mergers / merger remnants we can learn about the violent star formation history of these galaxies and eventually about galaxy formation and evolution. We will present a new set of evolutionary synthesis models of our GALEV code specially developed to account for the gaseous emission of presently forming star clusters and an advanced tool to compare large model grids with multi-color broad-band observations becoming presently available in large amounts. Such observations are an ecomonic way to determine the parameters of young star clusters as will be shown in the presentation. First results of newly-born clusters in mergers and starburst galaxies are presented and compared to the well-studied old globulars and interpreted in the framework of galaxy formation / evolution.

  1. Neutron star/red giant encounters in globular clusters

    International Nuclear Information System (INIS)

    Bailyn, C.D.

    1988-01-01

    The author presents a simple expression for the amount by which xsub(crit) is diminished as a star evolves xsub(crit) Rsub(crit)/R*, where Rsub(crit) is the maximum distance of closest approach between two stars for which the tidal energy is sufficient to bind the system, and R* is the radius of the star on which tides are being raised. Also it is concluded that tidal capture of giants by neutron stars resulting in binary systems is unlikely in globular clusters. However, collisions between neutron stars and red giants, or an alternative process involving tidal capture of a main-sequence star into an initially detached binary system, may result either in rapidly rotating neutron stars or in white dwarf/neutron star binaries. (author)

  2. Giant Rapid X-ray Flares in Extragalactic Globular Clusters

    Science.gov (United States)

    Irwin, Jimmy

    2018-01-01

    There is only one known class of non-destructive, highly energetic astrophysical object in the Universe whose energy emission varies by more than a factor of 100 on time scales of less than a minute -- soft gamma repeaters/anomalous X-ray pulsars, whose flares are believed to be caused by the energy release from the cracking of a neutron star's surface by very strong magnetic fields. All other known violent, rapid explosions, including gamma-ray bursts and supernovae, are believed to destroy the object in the process. Here, we report the discovery of a second class of non-destructive, highly energetic rapidly flaring X-ray object located within two nearby galaxies with fundamentally different properties than soft gamma repeaters/anomalous X-ray pulsars. One source is located within a suspected globular cluster of the host galaxy and flared one time, while the other source is located in either a globular cluster of the host galaxy or the core of a stripped dwarf companion galaxy that flared on six occasions over a seven year time span. When not flaring, the sources appear as normal accreting neutron star or black hole X-ray binaries, indicating that the flare event does not significantly disrupt the host system. While the nature of these sources is still unclear, the discovery of these sources in decade-old archival Chandra X-ray Observatory data illustrates the under-utilization of X-ray timing as a means to discover new classes of explosive events in the Universe.

  3. An updated survey of globular clusters in M 31. III. A spectroscopic metallicity scale for the Revised Bologna Catalog

    Science.gov (United States)

    Galleti, S.; Bellazzini, M.; Buzzoni, A.; Federici, L.; Fusi Pecci, F.

    2009-12-01

    Aims. We present a new homogeneous set of metallicity estimates based on Lick indices for the old globular clusters of the M 31 galaxy. The final aim is to add homogeneous spectroscopic metallicities to as many entries as possible of the Revised Bologna Catalog of M 31 clusters, by reporting Lick index measurements from any source (literature, new observations, etc.) on the same scale. Methods: New empirical relations of [Fe/H] as a function of [MgFe] and Mg2 indices are based on the well-studied galactic globular clusters, complemented with theoretical model predictions for -0.2≤ [Fe/H]≤ +0.5. Lick indices for M 31 clusters from various literature sources (225 clusters) and from new observations by our team (71 clusters) have been transformed into the Trager et al. system, yielding new metallicity estimates for 245 globular clusters of M 31. Results: Our values are in good agreement with recent estimates based on detailed spectral fitting and with those obtained from color magnitude diagrams of clusters imaged with the Hubble Space Telescope. The typical uncertainty on individual estimates is ≃±0.25 dex, as resulted from the comparison with metallicities derived from color magnitude diagrams of individual clusters. Conclusions: The metallicity distribution of M 31 globular cluster is briefly discussed and compared with that of the Milky Way. Simple parametric statistical tests suggest that the distribution is probably not unimodal. The strong correlation between metallicity and kinematics found in previous studies is confirmed. The most metal-rich GCs tend to be packed into the center of the system and to cluster tightly around the galactic rotation curve defined by the HI disk, while the velocity dispersion about the curve increases with decreasing metallicity. However, also the clusters with [Fe/H]<-1.0 display a clear rotation pattern, at odds with their Milky Way counterparts. Based on observations made at La Palma, at the Spanish Observatorio del Roque

  4. Evaluation of canine adverse food reactions by patch testing with single proteins, single carbohydrates and commercial foods.

    Science.gov (United States)

    Johansen, Cornelia; Mariani, Claire; Mueller, Ralf S

    2017-10-01

    Adverse food reaction (AFR) is an important differential diagnosis for the pruritic dog. It is usually diagnosed by feeding an elimination diet with a novel protein and carbohydrate source for eight weeks followed by subsequent food provocation. A previous study demonstrated that patch testing dogs with foods had a high sensitivity and negative predictability for selection of elimination diet ingredients. The aim of this study was to investigate patch testing with proteins, carbohydrates and dry commercial dog food in dogs to determine whether there was value in patch testing to aid the diagnosis of canine adverse food reaction. Twenty five privately owned dogs, with confirmed AFR, underwent provocation trials with selected food antigens and patch testing. For proteins, carbohydrates and dry dog food the sensitivity of patch testing was 100%, 70% and 22.2%, respectively; the negative predictive values of patch testing were 100%, 79% and 72%. The positive predictive values of patch testing for proteins and carbohydrates were 75% and 74%, respectively. This study confirmed that patch testing may be useful for the selection of a suitable protein source for an elimination diet in dogs with suspected AFR, but not as a diagnostic tool for canine AFR. Results for proteins are more reliable than for carbohydrates and the majority of positive patch test reactions were observed with raw protein. Patch testing with commercial dog food does not seem to be useful. © 2017 ESVD and ACVD.

  5. Measuring age differences among globular clusters having similar metallicities - A new method and first results

    International Nuclear Information System (INIS)

    Vandenberg, D.A.; Bolte, M.; Stetson, P.B.

    1990-01-01

    A color-difference technique for estimating the relative ages of globular clusters with similar chemical compositions on the basis of their CM diagrams is described and demonstrated. The theoretical basis and implementation of the procedure are explained, and results for groups of globular clusters with m/H = about -2, -1.6, and -1.3, and for two special cases (Palomar 12 and NGC 5139) are presented in extensive tables and graphs and discussed in detail. It is found that the more metal-deficient globular clusters are nearly coeval (differences less than 0.5 Gyr), whereas the most metal-rich globular clusters exhibit significant age differences (about 2 Gyr). This result is shown to contradict Galactic evolution models postulating halo collapse in less than a few times 100 Myr. 77 refs

  6. Globular bodies: a primary cause of the opacity in senile and diabetic posterior cortical subcapsular cataracts?

    Science.gov (United States)

    Creighton, M O; Trevithick, J R; Mousa, G Y; Percy, D H; McKinna, A J; Dyson, C; Maisel, H; Bradley, R

    1978-07-01

    We examined 9 cataracts from maturity onset diabetics and 4 senile posterior subcapsular cataracts by scanning electron microscopy, transmission electron microscopy, immunofluorescence for crystallin proteins and actin, histochemical methods and x-ray diffraction. The cataractous regions contained spherical globules up to 20 mu in diameter, often in a fibrous matrix. Some were extracellular Morgagnian globules, apparently formed by blebbing from the cell surface; others appeared to have been formed intracellularly. The area of globular degeneration was usually 300 mu deep, but had deeper fusiform extensions. Morphological changes in the cell cytoplasm varied according to their depth in the cataract. Electron microscopy showed intracellular and extracellular globules, many of them were bounded by lipid bilayer membranes. Immunofluorescent staining showed that all the globules contained gamma-crystallin; some contained alpha- and beta-crystallins and actin. All the globules contained higher concentrations of cysteine or cystine than the surrounding lens tissue but they did not react to stains for carbohydrate or calcium. X-ray diffraction studies showed that crystalline calcium salts were absent. Globules and cavities averaged 45% of the total area in cross section. Assuming an area of cataract to be 300 micron thick and that globules 1 mu in diameter scattered, while 2--20 mu in diameter reflected light, we calculated that light passing through such a thickness would be reduced by 65%. Thus the globules could account for most of the opacity of the cataractous area. Presumably the fibrous degeneration of the cells causes enough light scattering to account for the remainder of the reduction. Cataract patients complain of decreased visual acuity, a golden halo around objects, and difficulties when driving while facing oncoming traffic at night. These probably result from light scattering. In our previous experiments, globular bodies containing gamma-crystallin were

  7. A spectroscopic and photometric study of MSP companions in Galactic Globular Clusters

    OpenAIRE

    Cocozza, Gabriele

    2008-01-01

    This Thesis is devoted to the study of the optical companions of Millisecond Pulsars in Galactic Globular Clusters (GCs) as a part of a large project started at the Department of Astronomy of the Bologna University, in collaboration with other institutions (Astronomical Observatory of Cagliari and Bologna, University of Virginia), specifically dedicated to the study of the environmental effects on passive stellar evolution in galactic GCs. Globular Clusters are very efficien...

  8. Globular clusters as a source of X-ray emission from the neighbourhood of M87

    International Nuclear Information System (INIS)

    Fabian, A.C.; Pringle, J.E.; Rees, M.J.

    1976-01-01

    It is stated that the X-ray emission from globular clusters may be attributable to accretion on to compact objects, the accreting material being supplied from binary companions, or gas trapped in the potential well of the cluster. Counts of objects in the vicinity of the M87 have revealed that it has an extensive halo of globular clusters, the number of which may exceed 10,000 within a radius of 23 arc min. Most of these clusters may be explicable as a population effect, and the similarity of their optical properties to those of cluster in our own Galaxy suggests that they may also contain X-ray sources. The brighter globular clusters in M87 may, however, be substantially more X-ray luminous, and there may be proportionally more gas available in globular clusters in M87 compared with our Galaxy. The average X-ray luminosity of individual globular clusters may be of the order of 10 38 erg/sec., which raises the possibility that the integrated globular cluster emission may account for a substantial fraction of the X-ray emission observed from the region of M87. In support of this it is noted that the extended X-ray emission from the Virgo cluster is centered on M87, which lies approximately 45 arc min from the cluster centroid, and it is expected that the general X-ray emission from the globular cluster will appear to be smoothly and symmetrically distributed about M87 at moderate spatial resolution. A similar situation may apply to the elliptical galaxy NGC 3311 in Abell 1060 which, as a cluster, has been suggested as the identification for the X-ray source 3 U 1044-40, and it seems possible that that galaxy is surrounded by a similar globular cluster population to that of M87. (U.K.)

  9. Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions

    Science.gov (United States)

    Almer, Gunter; Saba-Lepek, Matthias; Haj-Yahya, Samih; Rohde, Eva; Strunk, Dirk; Fröhlich, Eleonore; Prassl, Ruth; Mangge, Harald

    2011-01-01

    Background: Adiponectin, an adipocyte-specific plasma protein, has been shown to accumulate in injured endothelial cells during development of atherosclerotic lesions. In this study, we investigated the potential of different adiponectin subfractions with special emphasis on globular adiponectin (gAd) to recognize and visualize atherosclerotic lesions. Methods: Recombinant mouse gAd and subfractions of full-length adiponectin (ie, trimeric, hexameric, and oligomeric forms) were fluorescence-labeled. Aortas of wild-type and apoprotein E-deficient mice fed a high cholesterol diet were dissected and incubated with the labeled biomarkers. Imaging was performed using confocal laser scanning microscopy. Results: Confocal laser scanning microscopic images showed that gAd binds more strongly to atherosclerotic plaques than full-length adiponectin subfractions. Further, we showed that gAd accumulates preferentially in endothelial cells and the fibrous cap area of plaques. Here we demonstrate for the first time that gAd recognizes atherosclerotic plaques on aortic sections of apoprotein E-deficient mice. Conclusion: These results suggest that gAd, in addition to its physiological properties, is also suitable as a target molecule for prospective diagnostic strategies in imaging atherosclerotic lesions. PMID:22022204

  10. Functional properties and sensory testing of whey protein concentrate sweetened with rebaudioside A

    Directory of Open Access Journals (Sweden)

    Paula Gimenez MILANI

    2016-02-01

    Full Text Available ABSTRACT Objective: To develop a natural dietary product with functional benefits for diabetic patients. Whey protein concentrate was obtained through the separation membrane processes and sweetened with rebaudioside A. This product was submitted to sensory testing in humans and used to evaluate possible functional properties in male Wistar rats models with diabetesMellitus induced by streptozotocin. Methods: Two concentrates were produced. Only the second showed protein content of 74.3 and 17.3% of lactose was used as supplementation in induced diabetic rats. This concentrate was obtained from the concentration by reverse osmosis system (180 k Daltons, followed by nanofiltration in a 500 k Daltons membrane and spray drying at 5.0% solution of the first concentrate developed. The concentrate was sweetened with rebaudioside A (rebaudioside A 26 mg/100 g concentrate. All procedures were performed at the Center for Studies in Natural Products, at the Universidade Estadual de Maringá. Three experimental groups were established (n=6: two groups of diabetic animals, one control group and one supplemented group; and a control group of normal mice (non-diabetic. The supplemented group received concentrates sweetened with rebaudioside A in a dose of 100 mg/kg bw/day by an esophageal tube for 35 days. Fasting, the fed state and body weight were assessed weekly for all groups. At the end of the supplementation period, the following were analyzed: plasma parameters of glucose, total cholesterol, triglycerides and fructosamine; the serum levels of aspartate aminotransferase and alanine aminotransferase, water and food intake. Organs and tissues were removed and weighed to assess mass and anatomical changes. Results: The product presented 74% of proteins and 17% of lactose and showed satisfactory sensory testing by the addition of 26 mg of rebaudioside A/100 g concentrate. Supplementation of the product reduced hyperglycemia, plasma fructosamine levels

  11. Fluorescence Microspectroscopy for Testing the Dimerization Hypothesis of BACE1 Protein in Cultured HEK293 Cells

    Science.gov (United States)

    Gardeen, Spencer; Johnson, Joseph L.; Heikal, Ahmed A.

    2016-06-01

    Alzheimer's Disease (AD) is a neurodegenerative disorder that results from the formation of beta-amyloid plaques in the brain that trigger the known symptoms of memory loss in AD patients. The beta-amyloid plaques are formed by the proteolytic cleavage of the amyloid precursor protein (APP) by the proteases BACE1 and gamma-secretase. These enzyme-facilitated cleavages lead to the production of beta-amyloid fragments that aggregate to form plaques, which ultimately lead to neuronal cell death. Recent detergent protein extraction studies suggest that BACE1 protein forms a dimer that has significantly higher catalytic activity than its monomeric counterpart. In this contribution, we examine the dimerization hypothesis of BACE1 in cultured HEK293 cells using complementary fluorescence spectroscopy and microscopy methods. Cells were transfected with a BACE1-EGFP fusion protein construct and imaged using confocal, and differential interference contrast to monitor the localization and distribution of intracellular BACE1. Complementary fluorescence lifetime and anisotropy measurements enabled us to examine the conformational and environmental changes of BACE1 as a function of substrate binding. Using fluorescence correlation spectroscopy, we also quantified the diffusion coefficient of BACE1-EGFP on the plasma membrane as a means to test the dimerization hypothesis as a fucntion of substrate-analog inhibitition. Our results represent an important first towards examining the substrate-mediated dimerization hypothesis of BACE1 in live cells.

  12. Sulphur in the metal poor globular cluster NGC 6397

    Science.gov (United States)

    Koch, A.; Caffau, E.

    2011-10-01

    Sulphur (S) is a non-refractory α-element that is not locked into dust grains in the interstellar medium. Thus no correction to the measured, interstellar sulphur abundance is needed and it can be readily compared to the S content in stellar photospheres. Here we present the first measurement of sulphur in the metal poor globular cluster (GC) NGC 6397, as detected in a MIKE/Magellan high signal-to-noise, high-resolution spectrum of one red giant star. While abundance ratios of sulphur are available for a larger number of Galactic stars down to an [Fe/H] of ~ -3.5 dex, no measurements in globular clusters more metal poor than -1.5 dex have been reported so far. We find aNLTE, 3-D abundance ratio of [S/Fe] = +0.52 ± 0.20 (stat.) ± 0.08 (sys.), based on theS I, Multiplet 1 line at 9212.8 Å. This value is consistent with a Galactic halo plateau as typical of other α-elements in GCs and field stars, but we cannot rule out its membership with a second branch of increasing [S/Fe] with decreasing [Fe/H], claimed in the literature, which leads to a large scatter at metallicities around - 2 dex. The [S/Mg] and [S/Ca] ratios in this star are compatible with a Solar value to within the (large) uncertainties. Despite the very large scatter in these ratios across Galactic stars between literature samples, this indicates that sulphur traces the chemical imprints of the other α-elements in metal poor GCs. Combined with its moderate sodium abundance ([S/Na]NLTE = 0.48), the [S/Fe] ratio in this GC extends a global, positive S-Na correlation that is not seen in field stars and might indicate that proton-capture reactions contributed to the production of sulphur in the (metal poor) early GC environments. This paper includes data gathered with the 6.5 m Magellan Telescopes located at Las Campanas Observatory, Chile.

  13. Identification of antigenic Sarcoptes scabiei proteins for use in a diagnostic test and of non-antigenic proteins that may be immunomodulatory

    Science.gov (United States)

    Morgan, Marjorie S.; Rider, S. Dean; Arlian, Larry G.

    2017-01-01

    Background Scabies, caused by the mite, Sarcoptes scabiei, infects millions of humans, and many wild and domestic mammals. Scabies mites burrow in the lower stratum corneum of the epidermis of the skin and are the source of substances that are antigenic or modulate aspects of the protective response of the host. Ordinary scabies is a difficult disease to diagnose. Objective The goal of this project was to identify S. scabiei proteins that may be candidate antigens for use in a diagnostic test or may be used by the mite to modulate the host’s protective response. Methods An aqueous extract of S. scabiei was separated by 2-dimensional electrophoresis and proteins were identified by mass spectrometry. A parallel immunoblot was probed with serum from patients with ordinary scabies to identify IgM and/or IgG-binding antigens. The genes coding for 23 selected proteins were cloned into E. coli and the expressed recombinant proteins were screened with serum from patients with confirmed ordinary scabies. Results We identified 50 different proteins produced by S. scabiei, 34 of which were not previously identified, and determined that 66% were recognized by patient IgM and/or IgG. Fourteen proteins were screened for use in a diagnostic test but none possessed enough sensitivity and specificity to be useful. Six of the 9 proteins selected for the possibility that they may be immunomodulatory were not recognized by antibodies in patient serum. Conclusions Thirty-three proteins that bound IgM and/or IgG from the serum of patients with ordinary scabies were identified. None of the 14 tested were useful for inclusion in a diagnostic test. The identities of 16 proteins that are not recognized as antigens by infected patients were also determined. These could be among the molecules that are responsible for this mite’s ability to modulate its host’s innate and adaptive immune responses. PMID:28604804

  14. Identification of antigenic Sarcoptes scabiei proteins for use in a diagnostic test and of non-antigenic proteins that may be immunomodulatory.

    Directory of Open Access Journals (Sweden)

    Marjorie S Morgan

    2017-06-01

    Full Text Available Scabies, caused by the mite, Sarcoptes scabiei, infects millions of humans, and many wild and domestic mammals. Scabies mites burrow in the lower stratum corneum of the epidermis of the skin and are the source of substances that are antigenic or modulate aspects of the protective response of the host. Ordinary scabies is a difficult disease to diagnose.The goal of this project was to identify S. scabiei proteins that may be candidate antigens for use in a diagnostic test or may be used by the mite to modulate the host's protective response.An aqueous extract of S. scabiei was separated by 2-dimensional electrophoresis and proteins were identified by mass spectrometry. A parallel immunoblot was probed with serum from patients with ordinary scabies to identify IgM and/or IgG-binding antigens. The genes coding for 23 selected proteins were cloned into E. coli and the expressed recombinant proteins were screened with serum from patients with confirmed ordinary scabies.We identified 50 different proteins produced by S. scabiei, 34 of which were not previously identified, and determined that 66% were recognized by patient IgM and/or IgG. Fourteen proteins were screened for use in a diagnostic test but none possessed enough sensitivity and specificity to be useful. Six of the 9 proteins selected for the possibility that they may be immunomodulatory were not recognized by antibodies in patient serum.Thirty-three proteins that bound IgM and/or IgG from the serum of patients with ordinary scabies were identified. None of the 14 tested were useful for inclusion in a diagnostic test. The identities of 16 proteins that are not recognized as antigens by infected patients were also determined. These could be among the molecules that are responsible for this mite's ability to modulate its host's innate and adaptive immune responses.

  15. Roles of beta-turns in protein folding: from peptide models to protein engineering.

    Science.gov (United States)

    Marcelino, Anna Marie C; Gierasch, Lila M

    2008-05-01

    Reverse turns are a major class of protein secondary structure; they represent sites of chain reversal and thus sites where the globular character of a protein is created. It has been speculated for many years that turns may nucleate the formation of structure in protein folding, as their propensity to occur will favor the approximation of their flanking regions and their general tendency to be hydrophilic will favor their disposition at the solvent-accessible surface. Reverse turns are local features, and it is therefore not surprising that their structural properties have been extensively studied using peptide models. In this article, we review research on peptide models of turns to test the hypothesis that the propensities of turns to form in short peptides will relate to the roles of corresponding sequences in protein folding. Turns with significant stability as isolated entities should actively promote the folding of a protein, and by contrast, turn sequences that merely allow the chain to adopt conformations required for chain reversal are predicted to be passive in the folding mechanism. We discuss results of protein engineering studies of the roles of turn residues in folding mechanisms. Factors that correlate with the importance of turns in folding indeed include their intrinsic stability, as well as their topological context and their participation in hydrophobic networks within the protein's structure.

  16. Diagnosis of protein-losing gastroenteropathy by 111In-transferrin scanning and fecal excretion test

    International Nuclear Information System (INIS)

    Urita, Yoshihisa

    1991-01-01

    A total of 12 patients with gastroenteropathy were studied for possible protein loss. The underlying diseases were intestinal lymphangiectasia, ulcerative colitis and Crohn's disease in 2 patients each; and Menetrier's disease, Cronkhite-Canada syndrome, postgastrectomy syndrome, severe atrophic gastritis, gastric cancer and gastroenteropathy of unknown origin in one patient each. The controls were four healthy volunteers and 9 patients with benign disease not accompanied by lesions in the digestive tract and protein loss, 111 In-transferrin (Tf) was prepared by incubating 2-3 mCi of 111 In-chloride with 15-20 ml of each patient's plasma in a sterilized vial at room temperature for 1 hour. After i.v. injection of 111 In-Tf, the patients were scanned at given intervals of time for 72 hours to localize protein loss. Seventy-two hour fecal 111 In-Tf excretion was also measured. 111 In-Tf was detected in the feces of all patients. The recovery rate in the patient group was 7.95±3.65%, being significantly higher than 0.48±0.26% in the control group. However, 111 In-Tf scanning showed the site of protein loss only in 6 patients. Negative scan in six other patients was thought to be associated with extensive inflammation and severe diarrhea. Five of the 12 patients were observed before and after treatment. A decrease in fecal 111 In-Tf excretion with clinical improvement was noted in the 5 patients after treatment. The site of protein loss could be demonstrated in 3 of the 5 patients before treatment. Its site in 1 of the 3 patients was the small intestine. However, accumulation of 111 In-Tf was found there in the 3 patients after treatment. The site of protein loss in one of three patients was the small intestine. 111 In-Tf scanning is particularly useful when the small intestine is involved, because surgery is contraindicated in this case. Fecal 111 In-Tf excretion test is mandatory when the site of protein loss cannot be imaged. (author)

  17. Platelet crossmatch tests using radiolabelled staphylococcal protein A or peroxidase anti-peroxidase in alloimmunised patients

    International Nuclear Information System (INIS)

    Yam, P.; Petz, L.D.; Scott, E.P.; Santos, S.

    1984-01-01

    Refractoriness to random-donor platelets as a result of alloimmunization remains a major problem in long-term platelet transfusion therapy despite the use of HLA-matched platelets. A study has been made of two methods for detection of platelet associated IgG as platelet crossmatch tests for the selection of platelet donors. These methods use radiolabelled staphylococcal protein A( 125 I-SPA) and peroxidase anti-peroxidase (PAP), respectively. One hundred and ten crossmatch tests using 125 I-SPA were performed retrospectively in 18 alloimmunized patients. The results indicated that the predictive value of a positive or a negative test was 87%; the sensitivity was 73% and the specificity was 95%. Results with the PAP test were similar. The HLA types were known for 48 donor-recipient pairs. With few exceptions, there was a correlation between the results of the platelet crossmatch tests and the effectiveness of platelet transfusion regardless of the degree of HLA match. These results indicate that platelet crossmatch tests may be valuable even when closely HLA matched donors are not available. A large-scale prospective study is warranted, particularly in highly immunized patients. (author)

  18. Acute Heat Stress Changes Protein Expression in the Testes of a Broiler-Type Strain of Taiwan Country Chickens.

    Science.gov (United States)

    Wang, Shih-Han; Cheng, Chuen-Yu; Chen, Chao-Jung; Chan, Hong-Lin; Chen, Hsin-Hsin; Tang, Pin-Chi; Chen, Chih-Feng; Lee, Yen-Pai; Huang, San-Yuan

    2018-03-19

    Heat stress leads to decreased fertility in roosters. This study investigated the global protein expression in response to acute heat stress in the testes of a broiler-type strain of Taiwan country chickens (TCCs). Twelve 45-week-old roosters were randomly allocated to the control group maintained at 25°C, and three groups subjected to acute heat stress at 38°C for 4 h, with 0, 2, and 6 h of recovery, respectively. Testis samples were collected for hematoxylin and eosin staining, apoptosis assay, and protein analysis. The results revealed 101 protein spots that differed significantly from the control following exposure to acute heat stress. The proteins that were differentially expressed participated mainly in protein metabolism and other metabolic processes, responses to stimuli, apoptosis, cellular organization, and spermatogenesis. Proteins that negatively regulate apoptosis were downregulated and proteins involved in autophagy and major heat shock proteins (HSP90α, HSPA5, and HSPA8) were upregulated in the testes of heat-stressed chickens. In conclusion, acute heat stress causes a change in protein expression in the testes of broiler-type B strain TCCs and may thus impair cell morphology, spermatogenesis, and apoptosis. The expression of heat shock proteins increased to attenuate the testicular injury induced by acute heat stress.

  19. A binary origin for 'blue stragglers' in globular clusters.

    Science.gov (United States)

    Knigge, Christian; Leigh, Nathan; Sills, Alison

    2009-01-15

    Blue stragglers in globular clusters are abnormally massive stars that should have evolved off the stellar main sequence long ago. There are two known processes that can create these objects: direct stellar collisions and binary evolution. However, the relative importance of these processes has remained unclear. In particular, the total number of blue stragglers found in a given cluster does not seem to correlate with the predicted collision rate, providing indirect support for the binary-evolution model. Yet the radial distributions of blue stragglers in many clusters are bimodal, with a dominant central peak: this has been interpreted as an indication that collisions do dominate blue straggler production, at least in the high-density cluster cores. Here we report that there is a clear, but sublinear, correlation between the number of blue stragglers found in a cluster core and the total stellar mass contained within it. From this we conclude that most blue stragglers, even those found in cluster cores, come from binary systems. The parent binaries, however, may themselves have been affected by dynamical encounters. This may be the key to reconciling all of the seemingly conflicting results found to date.

  20. THE DISCOVERY OF REMOTE GLOBULAR CLUSTERS IN M33

    International Nuclear Information System (INIS)

    Huxor, A.; Ferguson, A. M. N.; Barker, M. K.; Tanvir, N. R.; Irwin, M. J.; Chapman, S. C.; Ibata, R.; Lewis, G.

    2009-01-01

    We present the discovery of four remote star clusters in M33, one of which is of an extended nature. Three of the clusters were discovered using survey data from the Isaac Newton Telescope Wide-Field Camera while one was discovered serendipitously in a deep image taken with the Hubble Space Telescope's Advanced Camera for Surveys. With projected radii of 38-113 arcmin (9.6-28.5 kpc for an assumed M33 distance of 870 kpc), these clusters lie significantly beyond all but one of the currently confirmed clusters in M33. The clusters have magnitudes and colors consistent with their being old to intermediate-age globular clusters (GCs). Indeed, they bear a strong resemblance to the outer halo GC population of the Milky Way and M31 in terms (V - I) 0 color. The three outermost clusters are projected on the far side of M33 with respect to M31, an asymmetry that could suggest tidal interactions have affected M33's GC distribution at large radii.

  1. The X-ray globular cluster NGC 1851

    CERN Document Server

    Alcaino, G

    1976-01-01

    A BV photometric investigation of the Southern Globular Cluster NGC 1851, was carried out using the 1 m telescope of Cerro La Silla (ESO) for the photoelectric work and the 1 m telescope of Cerro Las Campanas (CARSO) for the photographic work. Nineteen stars were observed photoelectrically, the limiting magnitude being V=16.18. Using this sequence, 156 stars were measured photographically. The derived apparent distance modulus is (m-M)/sub app/=15/sup m/.50. The reddening is E(B-V)=0/sup m/.10. The true distance modulus is (m-M) /sub 0/=15/sup m/.20. The distance is 11 kpc from the sun, 6 kpc from the galactic plane and 17 kpc from the galactic centre. The main features of the colour-magnitude diagram are: a well defined horizontal branch abundant in red stars and deficient in blue stars, a rich subgiant and asymptotic branch and a moderately populated red giant branch of medium steepness rising to Delta V=2/sup m/.5 at (B-V) /sub 0/=1.4. At the distance of 11 kpc the maximum observed luminosity of the X-ray ...

  2. BVI CCD photometry of the globular cluster M4

    International Nuclear Information System (INIS)

    Alcaino, G.; Liller, W.; Alvarado, F.

    1988-01-01

    CCD BV1 main-sequence (MS) photometry of M4, the globular cluster closest to the sun, is presented. The photometry is matched to the BVI isochrones of VandenBerg and Bell (1985). The MS turnoffs are found to be at V = 16.90 + or - 0.05, B-V = 0.81 + or - 0.02, V-I = 0.96 + or - 0.02, and B - I = 1.77 + or - 0.02. The magnitude difference between the MS turnoff and the horizontal branch is Delta M(V) = 3.52 + or - 0.1 for all three color indices. Using Y = 0.2, (Fe/H) = - 1.27, and alpha = 1.65, with a distance modulus of (m-M)V = 12.7 and E(B-V) = 0.41, a consistent age for M4 is deduced in all three color indices of 17 + or - 1.5 Gyr. 34 references

  3. Deep CCD photometry in globular clusters. VII. M30

    International Nuclear Information System (INIS)

    Richer, H.B.; Fahlman, G.G.; Vandenberg, D.A.

    1988-01-01

    New UBV CCD photometry in a single field of the globular cluster M30 was obtained, and the data were used to obtain the color magnitude diagram (CMD) of the cluster, its luminosity function, and to derive fundamental cluster parameters. No blue stragglers were found, nor any evidence of a binary sequence in the data even though the field under study is only 21 core radii from the cluster center. The cluster reddening is observed to be 0.068 + or - 0.035, significantly higher than that adopted in most current papers on M30. An intercomparison of the CMDs of three very metal-poor clusters clearly shows that there is no evidence for any age difference between them. The age of M30 itself is found to be about 14 Gyr. The luminosity function of M30 is determined to be M(V) = 8. Comparison of this function with one found by Bolte (1987) at 65 core radii shows clear evidence of mass segregation in the low-mass stars. 44 references

  4. Evolution of highly compact binary stellar systems in globular clusters

    International Nuclear Information System (INIS)

    Krolik, J.H.; Meiksin, A.; Joss, P.C.

    1984-01-01

    We have calculated the secular evolution of a highly compact binary stellar system, composed of a collapsed object and a low-mass secondary star, in the core of a globular cluster. The binary evolves under the combined influences of (i) gravitational radiation losses from the system, (ii) the evolution of the secondary star, (iii) the resultant gradual mass transfer, if any, from the secondary to the collapsed object, and (iv) occasional encounters with passing field stars. We calculate all these effects in detail, utilizing some simplifying approximations appropriate to low-mass secondaries. The times of encounters with field stars, and the initial parameter specifying those encounters, were chosen by use of a Monte Carlo technique; the subsequent gravitational interactions were calculated utilzing a three-body integrator, and the changes in the binary orbital parmeters were thereby determined. We carried out a total of 20 such evolutionary calculations for each of two cluster core densities (1 and 3 x 10 3 stars pc -3 ). Each calculation was continued until the binary was disrupted or until 2 x 10 10 yr had elapsed

  5. KINEMATICS OF OUTER HALO GLOBULAR CLUSTERS IN M31

    International Nuclear Information System (INIS)

    Veljanoski, J.; Ferguson, A. M. N.; Bernard, E. J.; Peñarrubia, J.; Mackey, A. D.; Huxor, A. P.; Irwin, M. J.; Chapman, S. C.; Côté, P.; Tanvir, N. R.; McConnachie, A.; Ibata, R. A.; Martin, N. F.; Fardal, M.; Lewis, G. F.

    2013-01-01

    We present the first kinematic analysis of the far outer halo globular cluster (GC) population in the Local Group galaxy M31. Our sample contains 53 objects with projected radii of ∼20-130 kpc, 44 of which have no previous spectroscopic information. GCs with projected radii ∼> 30 kpc are found to exhibit net rotation around the minor axis of M31, in the same sense as the inner GCs, albeit with a smaller amplitude of 79 ± 19 km s –1 . The rotation-corrected velocity dispersion of the full halo GC sample is 106 ± 12 km s –1 , which we observe to decrease with increasing projected radius. We find compelling evidence for kinematic coherence among GCs that project on top of halo substructure, including a clear signature of infall for GCs lying along the northwest stream. Using the tracer mass estimator, we estimate the dynamical mass of M31 within 200 kpc to be M M31 = (1.2-1.5) ± 0.2 × 10 12 M ☉ . This value is highly dependent on the chosen model and assumptions within.

  6. Multicolor photometric study of M31 globular clusters

    International Nuclear Information System (INIS)

    Fan Zhou; Ma Jun; Zhou Xu

    2009-01-01

    We present the photometry of 30 globular clusters (GCs) and GC candidates in 15 intermediate-band filters covering the wavelength region from ∼3000 to ∼10000 A using the archival CCD images of M31 observed as part of the Beijing - Arizona - Taiwan - Connecticut (BATC) Multicolor Sky Survey. We transform these intermediate-band photometric data into the photometry in the standard U BV RI broad-bands. These M31 GC candidates are selected from the Revised Bologna Catalog (RBC V.3.5), and most of these candidates do not have any photometric data. Therefore, the presented photometric data are a supplement to the RBC V.3.5. We find that 4 out of 61 GCs and GC candidates in RBC V.3.5 do not show any signal on the BATC images at their locations. By applying a linear fit of the distribution in the color-magnitude diagram of blue GCs and GC candidates using data from the RBC V.3.5, in this study, we find the 'blue-tilt' of blue M31 GCs with a high confidence at 99.95% or 3.47σ for the confirmed GCs, and > 99.99% or 4.87σ for GCs and GC candidates. (research papers)

  7. Study of Remote Globular Cluster Satellites of M87

    Science.gov (United States)

    Sahai, Arushi; Shao, Andrew; Toloba, Elisa; Guhathakurta, Puragra; Peng, Eric W.; Zhang, Hao

    2017-01-01

    We present a sample of “orphan” globular clusters (GCs) with previously unknown parent galaxies, which we determine to be remote satellites of M87, a massive elliptical galaxy at the center of the Virgo Cluster of Galaxies. Because GCs were formed in the early universe along with their original parent galaxies, which were cannibalized by massive galaxies such as M87, they share similar age and chemical properties. In this study, we first confirm that M87 is the adoptive parent galaxy of our orphan GCs using photometric and spectroscopic data to analyze spatial and velocity distributions. Next, we increase the signal-to-noise ratio of our samples’ spectra through a process known as coaddition. We utilize spectroscopic absorption lines to determine the age and metallicity of our orphan GCs through comparison to stellar population synthesis models, which we then relate to the GCs’ original parent galaxies using a mass-metallicity relation. Our finding that remote GCs of M87 likely developed in galaxies with ~1010 solar masses implies that M87’s outer halo is formed of relatively massive galaxies, serving as important parameters for developing theories about the formation and evolution of massive galaxies.This research was funded in part by NASA/STScI and the National Science Foundation. Most of this work was carried out by high school students working under the auspices of the Science Internship Program at UC Santa Cruz.

  8. The luminosity function for globular clusters, 4: M3

    International Nuclear Information System (INIS)

    Simoda, Mahiro; Fukuoka, Takashi

    1976-01-01

    The subgiant-turnoff portion (V = 17.2 - 20.0 mag) of the luminosity function for the globular cluster M3 has been determined from photometry of the stars within the annuli 3'-8' and 6'-8' for V = 17.2 - 19.0 mag and 19.0 - 20.0 mag, respectively, by using plates taken with the Kitt Peak 2.1-m reflector. Our result shows that the luminosity function for M3 has a similar steep rise in the subgiant portion as other clusters so far studied (M5, M13, and M92), in direct conflict with the result by SANDAGE (1954, 1957). A probable cause of this discrepancy is given. Comparison with theoretical luminosity functions by SIMODA and IBEN (1970) suggests that theory and observation are not inconsistent if the initial helium abundance of M3 stars is taken to be about 20 percent. It is suggested that M13 has a larger helium abundance than M3 and M92 from the intercomparison of their luminosity functions and color-magnitude diagrams. (auth.)

  9. HUBBLE SPACE TELESCOPE PHOTOMETRY OF GLOBULAR CLUSTERS IN M81

    International Nuclear Information System (INIS)

    Nantais, Julie B.; Huchra, John P.; Zezas, Andreas; Gazeas, Kosmas; Strader, Jay

    2011-01-01

    We perform aperture photometry and profile fitting on 419 globular cluster (GC) candidates with m V ≤ 23 mag identified in Hubble Space Telescope/Advanced Camera for Surveys BVI imaging, and estimate the effective radii of the clusters. We identify 85 previously known spectroscopically confirmed clusters, and newly identify 136 objects as good cluster candidates within the 3σ color and size ranges defined by the spectroscopically confirmed clusters, yielding a total of 221 probable GCs. The luminosity function peak for the 221 probable GCs with estimated total dereddening applied is V ∼ (20.26 ± 0.13) mag, corresponding to a distance of ∼3.7 ± 0.3 Mpc. The blue and red GC candidates, and the metal-rich and metal-poor spectroscopically confirmed clusters, respectively, are similar in half-light radius. Red confirmed clusters are about 6% larger in median half-light radius than blue confirmed clusters, and red and blue good GC candidates are nearly identical in half-light radius. The total population of confirmed and 'good' candidates shows an increase in half-light radius as a function of galactocentric distance.

  10. Color Gradient in the King Type Globular Cluster NGC 7089

    Directory of Open Access Journals (Sweden)

    Young-Jong Sohn

    1999-12-01

    Full Text Available We use BV CCD images to investigate the reality of the color gradient within a King type globular cluster NGC 7089. Surface photometry shows that there is a strong radial color gradient in the central region of the cluster in the sense of bluer center with the amplitude of -0.39 +/- 0.07 mag/arcsec2 in (B - V. In the outer region of the cluster, however, the radial color gradient shows a reverse case, i.e., redder toward the center. (B - V color profile which was derived from resolved stars in VGC 7089 field also shows a significant color gradient in the central region of the clusters, indicating that lights from the combination of red giant stars and blue horizontal branch stars cause the radial color gradient. Color gradient of the outer region of NGC 7089 may be due to the unresolved background of the cluster. Similar color gradients in the central area of clusters have been previously observed exserved exclusively in highly concentrated systems classified as post core collapse clusters. We caution, however, to confirm the reality of the color gradient from resolved stars, we need more accurate imaging data of the cluster with exceptional seeing condition because the effect of completeness correlates with local density of stars.

  11. Spectroscopy of chromospheric lines of giants in the globular cluster

    Science.gov (United States)

    Dupree, A. K.; Hartmann, Lee; Smith, Graeme H.; Rodgers, A. W.; Roberts, W. H.; Zucker, D. B.

    1994-01-01

    Spectroscopic observations of chromospheric transitions (Mg II, H-alpha, and Ca II K) from two red giants (A31 and A59) in the globular cluster NGC 6572 were made with the Goddard High Resolution Spectrograph on the Hubble Space Telescope and the coude spectrograph of the 1.9 m telescope at the Mount Stromlo Observatory. These measurements give evidence for chromospheric activity and outward motions within the atmospheres. The surface flux of the Mg II emission is comparable to that in disk population giants of similar (B-V) color. The Mg II profiles are asymmetric, which is most likely caused by absorption in an expanding stellar atmosphere and/or by possible interstellar features. Notches are found in the core of the H-alpha line of A59, which are similar to those found in Cepheids. This suggests that shocks are present in the atmosphere of A59 and indicates that hydrodynamic phenomena are influencing the levvel of chromospheric emission and producing upper atmospheric motions which may lead to mass loss.

  12. Peculiarities of dynamic evaluation of globular formation outlines of the lungs with multislice computed tomography

    Directory of Open Access Journals (Sweden)

    Vladimir G. Kolmogorov

    2017-01-01

    Full Text Available Background. Visualization of infiltration in lung tissue surrounding the globular formation of the lungs (GFL determined by X-ray is one of the important points in the differential diagnosis of primary lung cancer, specific and non-specific inflammatory processes. At CT gauge body phantoms test facilities are widely used for evaluating the performance of scanners that allow the evaluation of scanner characteristics : noise, contrast sensitivity, positioning accuracy, stiffness of the radiation beam, the layer thickness, spatial resolution, etc.Aim. To develop a methodology for assessing the GFL outlines of the dynamics of multislice computed tomography (MSCT by selecting the optimal image processing algorithms.Materials and methods. The visual analysis of two- component physical model images of the electronic window level (WL and electronic window width (WW was installed on the basis of the best conditions for studying a specific group of tissues. In the case of indistinct, poorly defined outlines of globular formations, visual assessment is operator-dependent and requires development and application of quantitative methods of analysis. For a quantitative description of the outlines of the image of the GFL model, a vector in a polar coordinate system coming from the center of the figure mass bounded by the outline was used. The following outline complexity measures were adopted: modified Shannon information entropy H(S(k for k harmonics of the normalized spectral power density S(k of the length of oscillation of loop radius vector R(n; the number of local maxima L of signature radius vector R(n; the maximum value of the normalized power spectral density S(k; product (multiplicity of the entropy H(S and the number of local maxima L.Results. “Multiplicity”, “the number of local maxima” of the outline depend on the GFL geometric dimensions and cannot be used for diagnosis without first normalizing for GFL outline length. The parameters

  13. COMPARISON OF ALPHA-ELEMENT-ENHANCED SIMPLE STELLAR POPULATION MODELS WITH MILKY WAY GLOBULAR CLUSTERS

    International Nuclear Information System (INIS)

    Lee, Hyun-chul; Worthey, Guy; Dotter, Aaron

    2009-01-01

    We present simple stellar population (SSP) models with scaled-solar and α-element-enhanced abundances. The SSP models are based on the Dartmouth Stellar Evolution Database, our library of synthetic stellar spectra, and a detailed systematic variation of horizontal-branch (HB) morphology with age and metallicity. In order to test the relative importance of a variety of SSP model ingredients, we compare our SSP models with integrated spectra of 41 Milky Way globular clusters (MWGCs) from Schiavon et al. Using the Mg b and Ca4227 indices, we confirm that Mg and Ca are enhanced by about +0.4 and +0.2 dex, respectively, in agreement with results from high-resolution spectra of individual stars in MWGCs. Balmer lines, particularly Hγ and Hδ, of MWGCs are reproduced by our α-enhanced SSP models not only because of the combination of isochrone and spectral effects but also because of our reasonable HB treatment. Moreover, it is shown that the Mg abundance significantly influences Balmer and iron line indices. Finally, the investigation of power-law initial mass function (IMF) variations suggests that an IMF much shallower than Salpeter is unrealistic because the Balmer lines are too strong on the metal-poor side to be compatible with observations.

  14. Improving students’ creative thinking skill through local material-based experiment (LMBE) on protein qualitative test

    Science.gov (United States)

    Supriyanti, F. M. T.; Halimatul, H. S.

    2018-05-01

    This study aims to enhance chemistry students’ creative thinking skills using material from local resources on protein qualitative test experiment (LMBE). In this study, a quasi experiment method using one group pretest-postest non-equivalen control group design was carried out on the effectiveness of local material-based experiment approach. The data was collected using the test consists of five assay test and student work sheet (LKM). The effectiveness of the local material-based experiment was tested by means of percentage of normalized gain and score percentage of students’ worksheet. Comparison of creative thinking skills pretest and postest scores showed that the implementation of local material- based experiment (LMBE) enhanced students’s creative thinking skills in experiment class with the value of normalized gain (=0,77) at high category, while in control class reached to =0,44 at medium category. In addition, the LKM shown the enhancements of all aspect of creative thinking skills, including fluency, flexibility, and elaboration skills with the score of in experiment class are 0.79; 0.75; and 0.87 at high category, respectively. In contrast, the only the elaboration skill of control group was improved at high category (=0.76), while fluency and flexibility indicators enhanced at medium category (=0.48 and 0.56, respectively).

  15. PRED-CLASS: cascading neural networks for generalized protein classification and genome-wide applications.

    Science.gov (United States)

    Pasquier, C; Promponas, V J; Hamodrakas, S J

    2001-08-15

    A cascading system of hierarchical, artificial neural networks (named PRED-CLASS) is presented for the generalized classification of proteins into four distinct classes-transmembrane, fibrous, globular, and mixed-from information solely encoded in their amino acid sequences. The architecture of the individual component networks is kept very simple, reducing the number of free parameters (network synaptic weights) for faster training, improved generalization, and the avoidance of data overfitting. Capturing information from as few as 50 protein sequences spread among the four target classes (6 transmembrane, 10 fibrous, 13 globular, and 17 mixed), PRED-CLASS was able to obtain 371 correct predictions out of a set of 387 proteins (success rate approximately 96%) unambiguously assigned into one of the target classes. The application of PRED-CLASS to several test sets and complete proteomes of several organisms demonstrates that such a method could serve as a valuable tool in the annotation of genomic open reading frames with no functional assignment or as a preliminary step in fold recognition and ab initio structure prediction methods. Detailed results obtained for various data sets and completed genomes, along with a web sever running the PRED-CLASS algorithm, can be accessed over the World Wide Web at http://o2.biol.uoa.gr/PRED-CLASS.

  16. Rapid and quantitative detection of C-reactive protein using quantum dots and immunochromatographic test strips

    Directory of Open Access Journals (Sweden)

    Cheng X

    2014-12-01

    Full Text Available Xianglin Cheng,1,* Xu Pu,2,* Pen Jun,3 XiaoBo Zhu,3 Di Zhu,4 Ming Chen1 1Department of Laboratory Medicine, First Affiliated Hospital of Yangtze University, Jingzhou, 2Department of Laboratory Medicine, RenMin Hospital of Wuhan University, Wuhan, 3Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, People’s Republic of China; 4Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA *These authors contributed equally to this study and share first authorship Background: Rapid immunochromatographic tests can detect disease markers in 10–15 minutes, which facilitates clinical diagnosis and treatment programs. However, most immunochromatographic tests employ gold nanoparticles as reporters, and these have only moderate sensitivity and act as qualitative methods for analyzing high biomarker concentrations. Methods: In this study, we introduce quantum dots (QDs as fluorescent probes and immunochromatographic strips to develop quantitative fluorescence point-of-care tests (QF-POCT to analyze C-reactive protein (CRP levels. Goat anti-rabbit IgG and rabbit IgG were used as control antibodies, and mouse monoclonal CRP antibody pairs were used for disease marker detection. One monoclonal CRP antibody was conjugated with QDs and served as a signal antibody, and the other monoclonal CRP antibody was dispensed onto the nitrocellulose membrane and served as a capturing antibody. In the presence of CRP, the fluorescence intensity of the monoclonal antibody-CRP-monoclonal antibody sandwich complex captured on the nitrocellulose membrane was determined using the fluorescence strip reader. Results: QF-POCT assays could quantitatively analyze the concentration of CRP in 15 minutes had a detection limit of 0.25 mg/L, and had a wide detection linearity range (0.5–300 mg/L. The intra-assay and interassay

  17. Human diploid fibroblasts have receptors for the globular domain of C1Q

    International Nuclear Information System (INIS)

    Bordin, S.; Page, R.C.

    1986-01-01

    The authors showed that mass cultures of fibroblasts grown from gingival explants in DB medium with 10% human serum are enriched in a phenotype that binds C1q with an affinity much higher than the rest of the population. Because of potential biologic importance of C1q receptors, the authors studied whether the interaction between C1q and this phenotype was mediated by the globular or collagenous domains of the molecule. Globular fragments were prepared by digesting C1q with collagenase, and collagenous fragments obtained after pepsin treatment. C1q binding on cells in suspension was determined by reaction with 125 I-C1q as reported. Competition experiments were performed under conditions in which intact 125 I-C1q binding saturated all available receptors. The results showed that collagenous fragments inhibited 20% of the 125 I-C1q binding to high affinity receptors, whereas inhibition by globular fragments was 70%. Unlabeled intact C1q and collagen type 1 were used as controls, and inhibited 92% and 17% of C1q binding, respectively. These studies show that C1q interacts with the fibroblast phenotype expressing high affinity receptors through its globular domain. The authors suggest that at sites of trauma, native C1 may bind to the surface of these cells via the globular domain of C1q, and that this unique phenotype may play an important role in tissue repair

  18. Two stellar-mass black holes in the globular cluster M22.

    Science.gov (United States)

    Strader, Jay; Chomiuk, Laura; Maccarone, Thomas J; Miller-Jones, James C A; Seth, Anil C

    2012-10-04

    Hundreds of stellar-mass black holes probably form in a typical globular star cluster, with all but one predicted to be ejected through dynamical interactions. Some observational support for this idea is provided by the lack of X-ray-emitting binary stars comprising one black hole and one other star ('black-hole/X-ray binaries') in Milky Way globular clusters, even though many neutron-star/X-ray binaries are known. Although a few black holes have been seen in globular clusters around other galaxies, the masses of these cannot be determined, and some may be intermediate-mass black holes that form through exotic mechanisms. Here we report the presence of two flat-spectrum radio sources in the Milky Way globular cluster M22, and we argue that these objects are black holes of stellar mass (each ∼10-20 times more massive than the Sun) that are accreting matter. We find a high ratio of radio-to-X-ray flux for these black holes, consistent with the larger predicted masses of black holes in globular clusters compared to those outside. The identification of two black holes in one cluster shows that ejection of black holes is not as efficient as predicted by most models, and we argue that M22 may contain a total population of ∼5-100 black holes. The large core radius of M22 could arise from heating produced by the black holes.

  19. Images in the rocket ultraviolet - UV fluxes of M31 globular clusters

    International Nuclear Information System (INIS)

    Bohlin, R.C.; Cornett, R.H.; Hill, J.K.; Hill, R.S.; Stecher, T.P.

    1988-01-01

    Images obtained by a rocket-borne UV imaging telescope are used here to determine near-UV fluxes for 17 sources in M31 that are optical globular-cluster candidates and for the bright open cluster vdB0 in M31. Far-UV fluxes or flux limits are determined for the same clusters. The m(NUV)-V colors for M31 clusters are similar to those of Galactic clusters, except for the high-metallicity M31 cluster Bo 171. Four of the detected clusters have optical, m(NUV) - V, and m(FUV) - V colors indicating ages of about 100 million years. These four clusters are probably similar to the so-called 'blue globular' clusters of the LMC. The existence of young LMC-type blue globulars and the possible existence of middle-aged metal-rich globulars may indicate that M31 has continued to form globular clusters throughout its life. 39 references

  20. Identification of Hard X-ray Sources in Galactic Globular Clusters: Simbol-X Simulations

    Science.gov (United States)

    Servillat, M.

    2009-05-01

    Globular clusters harbour an excess of X-ray sources compared to the number of X-ray sources in the Galactic plane. It has been proposed that many of these X-ray sources are cataclysmic variables that have an intermediate magnetic field, i.e. intermediate polars, which remains to be confirmed and understood. We present here several methods to identify intermediate polars in globular clusters from multiwavelength analysis. First, we report on XMM-Newton, Chandra and HST observations of the very dense Galactic globular cluster NGC 2808. By comparing UV and X-ray properties of the cataclysmic variable candidates, the fraction of intermediate polars in this cluster can be estimated. We also present the optical spectra of two cataclysmic variables in the globular cluster M 22. The HeII (4868 Å) emission line in these spectra could be related to the presence of a magnetic field in these objects. Simulations of Simbol-X observations indicate that the angular resolution is sufficient to study X-ray sources in the core of close, less dense globular clusters, such as M 22. The sensitivity of Simbol-X in an extended energy band up to 80 keV will allow us to discriminate between hard X-ray sources (such as magnetic cataclysmic variables) and soft X-ray sources (such as chromospherically active binaries).

  1. Spectroscopic confirmation of the first symbiotic star in a globular cluster

    Science.gov (United States)

    Zurek, David

    2013-10-01

    We have recently discovered an 18-minute period in the ultraviolet of a star in the globular cluster NGC 1851. In the redder optical bands, this star is red and bright, while it shows a clear UV excess relative to other stars at similar positions in the HR diagram. The system is most likely a symbiotic binary, composed of a cool evolved star and a white dwarf, with an 18 minute spin period, accreting the cool star's wind. The binary would be the first such object ever found in a globular cluster, and only the third in the Galaxy where the white dwarf spin period is measured. The only viable alternatives are that the two components are a chance superposition - something with a nontrivial chance of happening in a globular cluster core. In such a case, the 18 minute period would most likely be the spin period of a magnetic white dwarf in an intermediate polar cataclysmic variable {this would be the first confirmed magnetic CV in a globular cluster}, or the orbital period of a double-degenerate AM CVn binary. Each of these three possibilities show unique {and very different} emission line spectra in the blue wavelength range. Two orbits of HST with STIS/G430L will produce a spectrum of sufficient signal-to-noise to distinguish between these 3 scenarios. The result will be an important constraint on N-body models of globular clusters.

  2. Why fibrous proteins are romantic.

    Science.gov (United States)

    Cohen, C

    1998-01-01

    Here I give a personal account of the great history of fibrous protein structure. I describe how Astbury first recognized the essential simplicity of fibrous proteins and their paradigmatic role in protein structure. The poor diffraction patterns yielded by these proteins were then deciphered by Pauling, Crick, Ramachandran and others (in part by model building) to reveal alpha-helical coiled coils, beta-sheets, and the collagen triple helical coiled coil-all characterized by different local sequence periodicities. Longer-range sequence periodicities (or "magic numbers") present in diverse fibrous proteins, such as collagen, tropomyosin, paramyosin, myosin, and were then shown to account for the characteristic axial repeats observed in filaments of these proteins. More recently, analysis of fibrous protein structure has been extended in many cases to atomic resolution, and some systems, such as "leucine zippers," are providing a deeper understanding of protein design than similar studies of globular proteins. In the last sections, I provide some dramatic examples of fibrous protein dynamics. One example is the so-called "spring-loaded" mechanism for viral fusion by the hemagglutinin protein of influenza. Another is the possible conformational changes in prion proteins, implicated in "mad cow disease," which may be related to similar transitions in a variety of globular and fibrous proteins. Copyright 1998 Academic Press.

  3. The globular cluster-dark matter halo connection

    Science.gov (United States)

    Boylan-Kolchin, Michael

    2017-12-01

    I present a simple phenomenological model for the observed linear scaling of the stellar mass in old globular clusters (GCs) with z = 0 halo mass in which the stellar mass in GCs scales linearly with progenitor halo mass at z = 6 above a minimum halo mass for GC formation. This model reproduces the observed MGCs-Mhalo relation at z = 0 and results in a prediction for the minimum halo mass at z = 6 required for hosting one GC: Mmin(z = 6) = 1.07 × 109 M⊙. Translated to z = 0, the mean threshold mass is Mhalo(z = 0) ≈ 2 × 1010 M⊙. I explore the observability of GCs in the reionization era and their contribution to cosmic reionization, both of which depend sensitively on the (unknown) ratio of GC birth mass to present-day stellar mass, ξ. Based on current detections of z ≳ 6 objects with M1500 10 are strongly disfavoured; this, in turn, has potentially important implications for GC formation scenarios. Even for low values of ξ, some observed high-z galaxies may actually be GCs, complicating estimates of reionization-era galaxy ultraviolet luminosity functions and constraints on dark matter models. GCs are likely important reionization sources if 5 ≲ ξ ≲ 10. I also explore predictions for the fraction of accreted versus in situ GCs in the local Universe and for descendants of systems at the halo mass threshold of GC formation (dwarf galaxies). An appealing feature of the model presented here is the ability to make predictions for GC properties based solely on dark matter halo merger trees.

  4. The Globular Cluster NGC 6402 (M14). II. Variable Stars

    Science.gov (United States)

    Contreras Peña, C.; Catelan, M.; Grundahl, F.; Stephens, A. W.; Smith, H. A.

    2018-03-01

    We present time-series BVI photometry for the Galactic globular cluster NGC 6402 (M14). The data consist of ∼137 images per filter, obtained using the 0.9 and 1.0 m SMARTS telescopes at the Cerro Tololo Inter-American Observatory. The images were obtained during two observing runs in 2006–2007. The image-subtraction package ISIS, along with DAOPHOT II/ALLFRAME, was used to perform crowded-field photometry and search for variable stars. We identified 130 variables, eight of which are new discoveries. The variable star population is comprised of 56 ab-type RR Lyrae stars, 54 c-type RR Lyrae, 6 type II Cepheids, 1 W UMa star, 1 detached eclipsing binary, and 12 long-period variables. We provide Fourier decomposition parameters for the RR Lyrae, and discuss the physical parameters and photometric metallicity derived therefrom. The M14 distance modulus is also discussed, based on different approaches for the calibration of the absolute magnitudes of RR Lyrae stars. The possible presence of second-overtone RR Lyrae in M14 is critically addressed, with our results arguing against this possibility. By considering all of the RR Lyrae stars as members of the cluster, we derive =0.589 {{d}}{{a}}{{y}}{{s}}. This, together with the position of the RR Lyrae stars of both Bailey types in the period–amplitude diagram, suggests an Oosterhoff-intermediate classification for the cluster. Such an intermediate Oosterhoff type is much more commonly found in nearby extragalactic systems, and we critically discuss several other possible indications that may point to an extragalactic origin for this cluster. Based on observations obtained with the 0.9 m and 1 m telescopes at the Cerro Tololo Inter-American Observatory, Chile, operated by the SMARTS consortium.

  5. THE DYNAMICAL EVOLUTION OF STELLAR BLACK HOLES IN GLOBULAR CLUSTERS

    Energy Technology Data Exchange (ETDEWEB)

    Morscher, Meagan; Pattabiraman, Bharath; Rodriguez, Carl; Rasio, Frederic A.; Umbreit, Stefan, E-mail: m.morscher@u.northwestern.edu [Center for Interdisciplinary Exploration and Research in Astrophysics (CIERA), Northwestern University, Evanston, IL (United States)

    2015-02-10

    Our current understanding of the stellar initial mass function and massive star evolution suggests that young globular clusters (GCs) may have formed hundreds to thousands of stellar-mass black holes (BHs), the remnants of stars with initial masses from ∼20-100 M {sub ☉}. Birth kicks from supernova explosions may eject some BHs from their birth clusters, but most should be retained. Using a Monte Carlo method we investigate the long-term dynamical evolution of GCs containing large numbers of stellar BHs. We describe numerical results for 42 models, covering a broad range of realistic initial conditions, including up to 1.6 × 10{sup 6} stars. In almost all models we find that significant numbers of BHs (up to ∼10{sup 3}) are retained all the way to the present. This is in contrast to previous theoretical expectations that most BHs should be ejected dynamically within a few gigayears The main reason for this difference is that core collapse driven by BHs (through the Spitzer {sup m}ass segregation instability{sup )} is easily reverted through three-body processes, and involves only a small number of the most massive BHs, while lower-mass BHs remain well-mixed with ordinary stars far from the central cusp. Thus the rapid segregation of stellar BHs does not lead to a long-term physical separation of most BHs into a dynamically decoupled inner core, as often assumed previously. Combined with the recent detections of several BH X-ray binary candidates in Galactic GCs, our results suggest that stellar BHs could still be present in large numbers in many GCs today, and that they may play a significant role in shaping the long-term dynamical evolution and the present-day dynamical structure of many clusters.

  6. Spatial Substructure in the M87 Globular Cluster System

    Science.gov (United States)

    Feng, Yuting; Zhang, Yunhao; Guhathakurta, Puragra; Peng, Eric; Lim, Sungsoon

    2018-01-01

    Based on the observation of Next Generation Virgo Cluster Survey (NGVS) project, we obtained the u,g,r,i,z and Ks band photometric information of all the objects in the 2 degree × 2 degree area (Pilot Region) around M87, the major subcluster of Virgo. By adapting an Extreme Deconvolution method, which classifies objects into Globular Clusters (GCs), galaxies and foreground stars with their color and morphology data, we got a purer-than-ever GC distribution map with a depth to gmag=25 in Pilot Region. After masking galaxy GCs, smoothing with a 10arcmin Gaussian kernel and performing a flat field correction, we show the GC density map of M87, and got a good sersic fitting of GC radial distribution with a sersic index~2.2 in the central ellipse part (45arcmin semi major axis area of M87). We quantitatively compared our GC sample with a substructure-free mock data set, which was generated from the smoothed density map as well as the sersic fitting, by calculating the 2 point correlation function (TPCF) value in different parts of the map. After separately performing such comparison with mocks based on different galaxy masking radii which vary from 4 times g band effective radius to 10, we found signals of remarkable spatial enhancement in certain directions in the central ellipse of M87, as well as halo substructures shown as lumpiness and holes in the outer region. We present the estimated scales of these substructures from the TPCF results, and, managed to locate them with a statistical analysis of the pixelized GC map. Apart from all results listed above, we discuss the constant, extra-galactic substructure signal at a scale of ~3kpc, which does not diminish with masking sizes, as the evidence of merging and accretion history of M87.

  7. BVRI CCD photometry of the globular cluster NGC 6362

    International Nuclear Information System (INIS)

    Alcaino, G.; Liller, W.

    1986-01-01

    We have obtained 78 BVRI CCD frames with the 1.54 m Danish telescope at ESO, La Silla, and have constructed V vs B-V, V vs V-R, V vs R-I, V vs V-I, and V vs B-I color-magnitude diagrams in a 4' x 2X5 field of the globular cluster NGC 6362. From these five CMDs we find that the main-sequence turnoffs are all close to the same magnitude, namely V/sub TO/ = 18.75 +- 0.1, and the color turn- offs at B-V = 0.50 +- 0.02, V-R = 0.31 +- 0.02, R-I = 0.35 +- 0.02, V-I = 0.68 +- 0.02, and B-I = 1.18 +- 0.03. The magnitude difference between the turnoff and the horizontal branch for the five diagrams is ΔM/sub V/ = 3.40 +- 0.15 in excellent agreement with the value given by Sandage (1982). Using Y = 0.2, Z = 0.001 ([Fe/H] = -1.27), α = 1.65, a distance modulus of (m-M)/sub V/ = 14.74, and E(B-V) = 0.10, we find that the VandenBerg and Bell isochrones (1985) yield a consistent age for NGC 6362 in all colors indexes of 16 +- 1.5 x 10 9 yr. The solar distance to the cluster is 7.7 kpc and the galactic distance is 5.6 kpc assuming R 0 = 9 kpc

  8. Globular Clusters: Absolute Proper Motions and Galactic Orbits

    Science.gov (United States)

    Chemel, A. A.; Glushkova, E. V.; Dambis, A. K.; Rastorguev, A. S.; Yalyalieva, L. N.; Klinichev, A. D.

    2018-04-01

    We cross-match objects from several different astronomical catalogs to determine the absolute proper motions of stars within the 30-arcmin radius fields of 115 Milky-Way globular clusters with the accuracy of 1-2 mas yr-1. The proper motions are based on positional data recovered from the USNO-B1, 2MASS, URAT1, ALLWISE, UCAC5, and Gaia DR1 surveys with up to ten positions spanning an epoch difference of up to about 65 years, and reduced to Gaia DR1 TGAS frame using UCAC5 as the reference catalog. Cluster members are photometrically identified by selecting horizontal- and red-giant branch stars on color-magnitude diagrams, and the mean absolute proper motions of the clusters with a typical formal error of about 0.4 mas yr-1 are computed by averaging the proper motions of selected members. The inferred absolute proper motions of clusters are combined with available radial-velocity data and heliocentric distance estimates to compute the cluster orbits in terms of the Galactic potential models based on Miyamoto and Nagai disk, Hernquist spheroid, and modified isothermal dark-matter halo (axisymmetric model without a bar) and the same model + rotating Ferre's bar (non-axisymmetric). Five distant clusters have higher-than-escape velocities, most likely due to large errors of computed transversal velocities, whereas the computed orbits of all other clusters remain bound to the Galaxy. Unlike previously published results, we find the bar to affect substantially the orbits of most of the clusters, even those at large Galactocentric distances, bringing appreciable chaotization, especially in the portions of the orbits close to the Galactic center, and stretching out the orbits of some of the thick-disk clusters.

  9. GLOBULAR CLUSTERS INDICATE THAT ULTRA-DIFFUSE GALAXIES ARE DWARFS

    Energy Technology Data Exchange (ETDEWEB)

    Beasley, Michael A.; Trujillo, Ignacio, E-mail: beasley@iac.es [Instituto de Astrofisica de Canarias, Calle Via Láctea, La Laguna, Tenerife (Spain)

    2016-10-10

    We present an analysis of archival HST /ACS imaging in the F475W ( g {sub 475}), F606W ( V {sub 606}), and F814W ( I {sub 814}) bands of the globular cluster (GC) system of a large (3.4 kpc effective radius) ultra-diffuse galaxy (DF17) believed to be located in the Coma Cluster of galaxies. We detect 11 GCs down to the 5 σ completeness limit of the imaging ( I {sub 814} = 27 mag). Correcting for background and our detection limits yields a total population of GCs in this galaxy of 27 ± 5 and a V -band specific frequency S {sub N} = 28 ± 5. Based on comparisons to the GC systems of local galaxies, we show that both the absolute number and the colors of the GC system of DF17 are consistent with the GC system of a dark-matter-dominated dwarf galaxy with virial mass ∼9.0 × 10{sup 10} M {sub ⊙} and a dark-to-stellar mass ratio M {sub vir}/ M {sub star} ∼ 1000. Based on the stellar mass growth of the Milky Way, we show that DF17 cannot be understood as a failed Milky-Way-like system, but is more similar to quenched Large-Magellanic-Cloud-like systems. We find that the mean color of the GC population, g {sub 475}– I {sub 814} = 0.91 ± 0.05 mag, coincides with the peak of the color distribution of intracluster GCs and is also similar to those of the blue GCs in the outer regions of massive galaxies. We suggest that both the intracluster GC population in Coma and the blue peak in the GC populations of massive galaxies may be fed—at least in part—by the disrupted equivalents of systems such as DF17.

  10. Protein hydrolysates are avoided by herbivores but not by omnivores in two-choice preference tests.

    Directory of Open Access Journals (Sweden)

    Kristin L Field

    Full Text Available The negative sensory properties of casein hydrolysates (HC often limit their usage in products intended for human consumption, despite HC being nutritious and having many functional benefits. Recent, but taxonomically limited, evidence suggests that other animals also avoid consuming HC when alternatives exist.We evaluated ingestive responses of five herbivorous species (guinea pig, mountain beaver, gopher, vole, and rabbit and five omnivorous species (rat, coyote, house mouse, white-footed mouse, and deer mouse; N = 16-18/species using solid foods containing 20% HC in a series of two-choice preference tests that used a non-protein, cellulose-based alternative. Individuals were also tested with collagen hydrolysate (gelatin; GE to determine whether it would induce similar ingestive responses to those induced by HC. Despite HC and GE having very different nutritional and sensory qualities, both hydrolysates produced similar preference score patterns. We found that the herbivores generally avoided the hydrolysates while the omnivores consumed them at similar levels to the cellulose diet or, more rarely, preferred them (HC by the white-footed mouse; GE by the rat. Follow-up preference tests pairing HC and the nutritionally equivalent intact casein (C were performed on the three mouse species and the guinea pigs. For the mice, mean HC preference scores were lower in the HC v C compared to the HC v Cel tests, indicating that HC's sensory qualities negatively affected its consumption. However, responses were species-specific. For the guinea pigs, repeated exposure to HC or C (4.7-h sessions; N = 10 were found to increase subsequent HC preference scores in an HC v C preference test, which was interpreted in the light of conservative foraging strategies thought to typify herbivores.This is the first empirical study of dietary niche-related taxonomic differences in ingestive responses to protein hydrolysates using multiple species under comparable

  11. [Lymphocyte transformation test following stimulation with a protein factor from neutrophilic granulocytes (PMNL) in psoriasis patients].

    Science.gov (United States)

    Ruszczak, Z; Ciborska, L; Kaszuba, A

    1988-12-01

    The lymphocyte transformation test (LTT) was given to 20 healthy subjects and 43 patients with generalized psoriasis vulgaris: it was given right after stimulation with PHA (spontaneous) and after stimulation with allogenic and autogenic protein factor (NPF). NPF was isolated from secondary lysosome granules of peripheral blood neutrophils. The results were analyzed using computer statistic tests. No distinct differences were noticed between the spontaneous transformation test in psoriatic patients compared to the controls. After stimulation with PHA, the percentage of blast cells was significantly lower in patients with psoriasis. When allogenic and autogenic NPF was used for stimulation, the LTT values were significantly higher in the psoriasis group than in the control subjects. This fact points out the increase in sensitivity of lymphocytes to NPF in active psoriasis and the possibility of abnormal neutrophil-lymphocyte interactions in vivo. This phenomenon may be intensified when under the influence of bacterial or viral agents, or medicaments; the degranulation of secondary lysosome granules of neutrophils occurs, causing the release of NPF. These investigations support our opinion that psoriasis is a systemic disease and that NPF plays a considerable role in the psoriatic reaction.

  12. Detection of anti neutrophil antibodies by radio-iodinated protein A binding test

    International Nuclear Information System (INIS)

    Cartron, J.; Muller, J.Y.; Tchernia, G.; Paule, B.; Varet, B.

    1983-01-01

    The granulocyte associated IgG in normal and neutropenic subjects has been determined by a direct quantitative assay using radiolabeled staphylococcal protein A. This assay allows to postulate an immunological mechanism to explain the neutropenia in 19 cases of neutropenias associated with malfunctions of the immune system and in 4 cases of idiopathic neutropenias. Discussed in this report is the possible interaction of immune complexes bound in vivo to the granulocytes. By an immunofluorescence test, it has been possible to detect IgG or IgM antibodies in only 52% of patients with a positive direct assay. The determination of granulocyte-associated IgG is therefore a better indicator for defining an auto-immune neutropenia than the detection of free antibodies in the sera [fr

  13. On the incidence of close binary stars in globular clusters and the nature of the cluster X-ray sources

    International Nuclear Information System (INIS)

    Trimble, V.

    1977-01-01

    Recent calculations suggest that the globular clusters could not have formed with more than 20 per cent of the normal Population I fraction of their stars in binary systems. The fact that the clusters have more than their fair share of novae and U Geminorum stars (three each out of approximately 200 of each known, while the clusters contain only about 10 -4 of the mass and 10 -3 of the luminosity of the galaxy) therefore becomes surprising. The hypothesis of binary capture within cluster cores suggested to account for the clusters' high X-ray luminosity provides a few extra systems, but neither it nor any of the similar encounter or capture mechanisms suggested can account for the novae and U Gen stars, which remain puzzling. The number of Algol-type and W UMa eclipsing binaries predicted by these hypotheses do not conflict with data presently available, but careful searches for them would constitute a critical test of the theories. (author)

  14. Binary Black Hole Mergers from Globular Clusters: Implications for Advanced LIGO.

    Science.gov (United States)

    Rodriguez, Carl L; Morscher, Meagan; Pattabiraman, Bharath; Chatterjee, Sourav; Haster, Carl-Johan; Rasio, Frederic A

    2015-07-31

    The predicted rate of binary black hole mergers from galactic fields can vary over several orders of magnitude and is extremely sensitive to the assumptions of stellar evolution. But in dense stellar environments such as globular clusters, binary black holes form by well-understood gravitational interactions. In this Letter, we study the formation of black hole binaries in an extensive collection of realistic globular cluster models. By comparing these models to observed Milky Way and extragalactic globular clusters, we find that the mergers of dynamically formed binaries could be detected at a rate of ∼100 per year, potentially dominating the binary black hole merger rate. We also find that a majority of cluster-formed binaries are more massive than their field-formed counterparts, suggesting that Advanced LIGO could identify certain binaries as originating from dense stellar environments.

  15. A NEW DISTANT MILKY WAY GLOBULAR CLUSTER IN THE PAN-STARRS1 3π SURVEY

    International Nuclear Information System (INIS)

    Laevens, Benjamin P. M.; Martin, Nicolas F.; Sesar, Branimir; Rix, Hans-Walter; Schlafly, Edward F.; Bernard, Edouard J.; Ferguson, Annette M. N.; Slater, Colin T.; Bell, Eric F.; Burgett, William S.; Chambers, Kenneth C.; Denneau, Larry; Kaiser, Nicholas; Kudritzki, Rolf-Peter; Magnier, Eugene A.; Morgan, Jeffrey S.; Sweeney, William E.; Draper, Peter W.; Metcalfe, Nigel; Price, Paul A.

    2014-01-01

    We present a new satellite in the outer halo of the Galaxy, the first Milky Way satellite found in the stacked photometric catalog of the Panoramic Survey Telescope and Rapid Response System 1 (Pan-STARRS1) Survey. From follow-up photometry obtained with WFI on the MPG/ESO 2.2 m telescope, we argue that the object, located at a heliocentric distance of 145 ± 17 kpc, is the most distant Milky Way globular cluster yet known. With a total magnitude of M V = –4.3 ± 0.2 and a half-light radius of 20 ± 2 pc, it shares the properties of extended globular clusters found in the outer halo of our Galaxy and the Andromeda galaxy. The discovery of this distant cluster shows that the full spatial extent of the Milky Way globular cluster system has not yet been fully explored

  16. The ellipticities of a sample of globular clusters in M31

    International Nuclear Information System (INIS)

    Lupton, R.H.

    1989-01-01

    Images for a sample of 18 globular clusters in M31 have been obtained. The mean ellipticity on the sky in the range 7-14 pc (2-4 arcsec) is 0.08 + or - 0.02 and 0.12 + or - 0.01 in the range 14-21 pc (4-6 arcsec), with corresponding true ellipticities of 0.12 and 0.18. The difference between the inner and outer parts is significant at a 99 percent level. The flattening of the inner parts is statistically indistinguishable from that of the Galactic globular clusters, while the outer parts are flatter than the Galactic clusters at a 99.8 percent confidence level. There is a significant anticorrelation of ellipticity with line strength; such a correlation may in retrospect also be seen in the Galactic globular cluster system. For the M31 data, this anticorrelation is stronger in the inner parts of the galaxy. 30 refs

  17. NO EVIDENCE FOR INTERMEDIATE-MASS BLACK HOLES IN GLOBULAR CLUSTERS: STRONG CONSTRAINTS FROM THE JVLA

    International Nuclear Information System (INIS)

    Strader, Jay; Chomiuk, Laura; Maccarone, Thomas J.; Miller-Jones, James C. A.; Seth, Anil C.; Heinke, Craig O.; Sivakoff, Gregory R.

    2012-01-01

    With a goal of searching for accreting intermediate-mass black holes (IMBHs), we report the results of ultra-deep Jansky Very Large Array radio continuum observations of the cores of three Galactic globular clusters: M15, M19, and M22. We reach rms noise levels of 1.5-2.1 μJy beam –1 at an average frequency of 6 GHz. No sources are observed at the center of any of the clusters. For a conservative set of assumptions about the properties of the accretion, we set 3σ upper limits on IMBHs from 360 to 980 M ☉ . These limits are among the most stringent obtained for any globular cluster. They add to a growing body of work that suggests either (1) IMBHs ∼> 1000 M ☉ are rare in globular clusters or (2) when present, IMBHs accrete in an extraordinarily inefficient manner.

  18. No Evidence for Intermediate-mass Black Holes in Globular Clusters: Strong Constraints from the JVLA

    Science.gov (United States)

    Strader, Jay; Chomiuk, Laura; Maccarone, Thomas J.; Miller-Jones, James C. A.; Seth, Anil C.; Heinke, Craig O.; Sivakoff, Gregory R.

    2012-05-01

    With a goal of searching for accreting intermediate-mass black holes (IMBHs), we report the results of ultra-deep Jansky Very Large Array radio continuum observations of the cores of three Galactic globular clusters: M15, M19, and M22. We reach rms noise levels of 1.5-2.1 μJy beam-1 at an average frequency of 6 GHz. No sources are observed at the center of any of the clusters. For a conservative set of assumptions about the properties of the accretion, we set 3σ upper limits on IMBHs from 360 to 980 M ⊙. These limits are among the most stringent obtained for any globular cluster. They add to a growing body of work that suggests either (1) IMBHs >~ 1000 M ⊙ are rare in globular clusters or (2) when present, IMBHs accrete in an extraordinarily inefficient manner.

  19. Changes in the protein profile of Habanero pepper ( Capsicum ...

    African Journals Online (AJOL)

    Protein profile was studied during the development of Capsicum chinense somatic embryos. The total protein content and profile of polypeptides (by sodium dodecyl sulfate polyacrylamide gel electrophoresis) of somatic embryos at different developmental stages (globular, heart-shaped, torpedo and cotyledonary stages) ...

  20. The Most Massive Star Clusters: Supermassive Globular Clusters or Dwarf Galaxy Nuclei?

    Science.gov (United States)

    Harris, William

    2004-07-01

    Evidence is mounting that the most massive globular clusters, such as Omega Centauri and M31-G1, may be related to the recently discovered "Ultra-Compact Dwarfs" and the dense nuclei of dE, N galaxies. However, no systematic imaging investigation of these supermassive globular clusters - at the level of Omega Cen and beyond - has been done, and we do not know what fraction of them might bear the signatures {such as large effective radii or tidal tails} of having originated as dE nuclei. We propose to use the ACS/WFC to obtain deep images of 18 such clusters in NGC 5128 and M31, the two nearest rich globular cluster systems. These globulars are the richest star clusters that can be found in nature, the biggest of them reaching 10^7 Solar masses, and they are likely to represent the results of star formation under the densest and most extreme conditions known. Using the profiles of the clusters including their faint outer envelopes, we will carry out state-of-the-art dynamical modelling of their structures, and look for any clear evidence which would indicate that they are associated with stripped satellites. This study will build on our previous work with STIS and WFPC2 imaging designed to study the 'Fundamental Plane' of globular clusters. When our new work is combined with Archival WFPC2, STIS, and ACS material, we will also be able to construct the definitive mapping of the Fundamental Plane of globular clusters at its uppermost mass range, and confirm whether or not the UCD and dE, N objects occupy a different structural parameter space.

  1. The Age of Globular Clusters in Light of Hipparcos: Resolving the Age Problem?

    Science.gov (United States)

    Chaboyer, Brian; Demarque, P.; Kernan, Peter J.; Krauss, Lawrence M.

    1998-02-01

    We review five independent techniques that are used to set the distance scale to globular clusters, including subdwarf main-sequence fitting utilizing the recent Hipparcos parallax catalog. These data together all indicate that globular clusters are farther away than previously believed, implying a reduction in age estimates. We now adopt a best-fit value Mv (RR Lyrae stars) = 0.39 +/- 0.08 (statistical) at [Fe/H] = -1.9 with an additional uniform systematic uncertainty of +0.13-0.18. This new distance scale estimate is combined with a detailed numerical Monte Carlo study (previously reported by Chaboyer et al.) designed to assess the uncertainty associated with the theoretical age-turnoff luminosity relationship in order to estimate both the absolute age and uncertainty in age of the oldest globular clusters. Our best estimate for the mean age of the oldest globular clusters is now 11.5 +/- 1.3 Gyr, with a one-sided 95% confidence level lower limit of 9.5 Gyr. This represents a systematic shift of over 2 σ compared to our earlier estimate, owing completely to the new distance scale--a shift which we emphasize results not only from the Hipparcos data. This now provides a lower limit on the age of the universe that is consistent with either an open universe or with a flat matter-dominated universe (the latter requiring H0 explicitly quantifies how remaining uncertainties in the distance scale and stellar evolution models translate into uncertainties in the derived globular cluster ages. Simple formulae are provided that can be used to update our age estimate as improved determinations for various quantities become available. Formulae are also provided that can be used to derive the age and its uncertainty for a globular cluster, given the absolute magnitude of the turnoff or the point on the subgiant branch 0.05 mag redder than the turnoff.

  2. Assessment of a Protein Cocktail-Based Skin Test for Bovine Tuberculosis in a Double-Blind Field Test in Cattle

    Science.gov (United States)

    Xin, Ting; Jia, Hong; Ding, Jiabo; Li, Pingjun; Yang, Hongjun; Hou, Shaohua; Yuan, Weifeng; Guo, Xiaoyu; Wang, Haichun; Liang, Qianqian; Li, Ming

    2013-01-01

    Bovine tuberculosis (bTB) is a worldwide zoonosis caused mainly by Mycobacterium bovis. The traditional diagnostic method used often is the tuberculin skin test, which uses bovine purified protein derivatives (PPD-B). However, it is difficult to maintain uniformity of PPD-B from batch to batch, and it shares common antigens with nonpathogenic environmental mycobacteria. To overcome these problems, M. bovis-specific antigens that showed good T cell stimulation, such as CFP-10, ESAT-6, Rv3615c, etc., have been used in the skin test, but there have been no large-scale clinical studies on these antigens. In this study, two combinations (CFP-10/ESAT-6/TB10.4 protein cocktail and CFP-10/ESAT-6/Rv3872/MPT63 protein cocktail) were developed and used as stimuli in the skin test. Cattle were double-blind tested to assess the efficiency of the protein cocktail-based skin tests. The results showed that the CFP-10/ESAT-6/TB10.4 protein cocktail-based skin test can differentiate TB-infected cattle from Mycobacterium avium-infected ones and that it shows a high degree of agreement with the traditional tuberculin skin test (κ = 0.8536) and gamma interferon (IFN-γ) release assay (κ = 0.8154). Compared to the tuberculin skin test, the relative sensitivity and relative specificity of the CFP-10/ESAT-6/TB10.4-based skin test were 87% and 97%, respectively., The relative sensitivity and relative specificity of the CFP-10/ESAT-6/TB10.4-based skin test were 93% and 92%, respectively, on comparison with the IFN-γ release assay. The correlation between the increases in skin thickness observed after the inoculation of stimuli was high (PPD-B versus CFP-10/ESAT-6/TB10.4, Spearman r of 0.8435). The correlation between the optical density at 450 nm (OD450) obtained after blood stimulation with PPD-B and the increase in skin thickness observed after inoculation of the CFP-10/ESAT-6/TB10.4 protein cocktail was high (Spearman r = 0.7335). Therefore, the CFP-10/ESAT-6/TB10.4-based skin test

  3. Keck Spectroscopy of Globular Clusters in the Elliptical Galaxy NGC 3610

    OpenAIRE

    Strader, Jay; Brodie, Jean P.; Schweizer, Francois; Larsen, Soeren S.; Seitzer, Patrick

    2002-01-01

    We present moderate-resolution Keck spectra of nine candidate globular clusters in the possible merger-remnant elliptical galaxy NGC 3610. Eight of the objects appear to be bona fide globular clusters of NGC 3610. We find that two of the clusters belong to an old metal-poor population, five to an old metal-rich population, and only one to an intermediate-age metal-rich population. The estimated age of the intermediate-age cluster is 1-5 Gyr, which is in agreement with earlier estimates of the...

  4. The Luminosity Functions of Old and Intermediate-Age Globular Clusters in NGC 3610

    OpenAIRE

    Whitmore, B. C.; Schweizer, F.; Kundu, A.; Miller, B. W.

    2002-01-01

    The WFPC2 Camera on board HST has been used to obtain high-resolution images of NGC 3610, a dynamically young elliptical galaxy. These observations supersede shorter, undithered HST observations where an intermediate-age population of globular clusters was first discovered. The new observations show the bimodal color distribution of globular clusters more clearly, with peaks at (V-I)o = 0.95 and 1.17. The luminosity function (LF) of the blue, metal-poor population of clusters in NGC 3610 turn...

  5. Constraints from stellar models on mixing as a viable explanation of abundance anomalies in globular clusters

    International Nuclear Information System (INIS)

    Vandenberg, D.A.; Smith, G.H.

    1988-01-01

    Published observational data on changes in the surface abundances of evolving stars in globular clusters are compiled and compared with the predictions of theoretical evolutionary sequences (for stars of mass 0.8 solar mass and metallicity Z = 0.0001 or mass 0.9 solar mass and Z = 0.006) and of models incorporating enhanced envelope-interior mixing at various evolutionary phases. The results are presented in graphs and characterized in detail. It is found that mixing models of CN bimodality in globular-cluster stars can encounter difficulties when abundance anomalies appear early in the evolution of the star. 63 references

  6. Engineering Protein Hydrogels Using SpyCatcher-SpyTag Chemistry.

    Science.gov (United States)

    Gao, Xiaoye; Fang, Jie; Xue, Bin; Fu, Linglan; Li, Hongbin

    2016-09-12

    Constructing hydrogels from engineered proteins has attracted significant attention within the material sciences, owing to their myriad potential applications in biomedical engineering. Developing efficient methods to cross-link tailored protein building blocks into hydrogels with desirable mechanical, physical, and functional properties is of paramount importance. By making use of the recently developed SpyCatcher-SpyTag chemistry, we successfully engineered protein hydrogels on the basis of engineered tandem modular elastomeric proteins. Our resultant protein hydrogels are soft but stable, and show excellent biocompatibility. As the first step, we tested the use of these hydrogels as a drug carrier, as well as in encapsulating human lung fibroblast cells. Our results demonstrate the robustness of the SpyCatcher-SpyTag chemistry, even when the SpyTag (or SpyCatcher) is flanked by folded globular domains. These results demonstrate that SpyCatcher-SpyTag chemistry can be used to engineer protein hydrogels from tandem modular elastomeric proteins that can find applications in tissue engineering, in fundamental mechano-biological studies, and as a controlled drug release vehicle.

  7. Metallicity Variations in the Type II Globular Cluster NGC 6934

    Science.gov (United States)

    Marino, A. F.; Yong, D.; Milone, A. P.; Piotto, G.; Lundquist, M.; Bedin, L. R.; Chené, A.-N.; Da Costa, G.; Asplund, M.; Jerjen, H.

    2018-06-01

    The Hubble Space Telescope photometric survey of Galactic globular clusters (GCs) has revealed a peculiar “chromosome map” for NGC 6934. In addition to a typical sequence, similar to that observed in Type I GCs, NGC 6934 displays additional stars on the red side, analogous to the anomalous Type II GCs, as defined in our previous work. We present a chemical abundance analysis of four red giants in this GC. Two stars are located on the chromosome map sequence common to all GCs, and another two lie on the additional sequence. We find (i) star-to-star Fe variations, with the two anomalous stars being enriched by ∼0.2 dex. Because of our small-size sample, this difference is at the ∼2.5σ level. (ii) There is no evidence for variations in the slow neutron-capture abundances over Fe, at odds with what is often observed in anomalous Type II GCs, e.g., M 22 and ω Centauri (iii) no large variations in light elements C, O, and Na, compatible with locations of the targets on the lower part of the chromosome map where such variations are not expected. Since the analyzed stars are homogeneous in light elements, the only way to reproduce the photometric splits on the sub-giant (SGB) and the red giant (RGB) branches is to assume that red RGB/faint SGB stars are enhanced in [Fe/H] by ∼0.2. This fact corroborates the spectroscopic evidence of a metallicity variation in NGC 6934. The observed chemical pattern resembles only partially the other Type II GCs, suggesting that NGC 6934 might belong either to a third class of GCs, or be a link between normal Type I and anomalous Type II GCs. Based on observations with the NASA/ESA Hubble Space Telescope, obtained at the Space Telescope Science Institute, which is operated by AURA, Inc., under NASA contract NAS 5-26555. This paper includes data gathered with the 6.5 m Magellan Telescopes located at Las Campanas Observatory, Chile, and Gemini Telescope at Canada–France–Hawaii Telescope.

  8. THE ORIGIN OF GAMMA RAYS FROM GLOBULAR CLUSTERS

    International Nuclear Information System (INIS)

    Cheng, K. S.; Chernyshov, D. O.; Dogiel, V. A.; Hui, C. Y.; Kong, A. K. H.

    2010-01-01

    Fermi has detected gamma-ray emission from eight globular clusters (GCs). It is commonly believed that the energy sources of these gamma rays are millisecond pulsars (MSPs) inside GCs. Also it has been standard to explain the spectra of most Fermi Large Area Telescope pulsars including MSPs resulting from the curvature radiation (CR) of relativistic electrons/positrons inside the pulsar magnetosphere. Therefore, gamma rays from GCs are expected to be the collection of CR from all MSPs inside the clusters. However, the angular resolution is not high enough to pinpoint the nature of the emission. In this paper, we calculate the gamma rays produced by the inverse Compton (IC) scattering between relativistic electrons/positrons in the pulsar wind of MSPs in the GCs and background soft photons including cosmic microwave/relic photons, background star lights in the clusters, the galactic infrared photons, and the galactic star lights. We show that the gamma-ray spectrum from 47 Tucanae can be explained equally well by upward scattering of either the relic photons, the galactic infrared photons, or the galactic star lights, whereas the gamma-ray spectra from the other seven GCs are best fitted by the upward scattering of either the galactic infrared photons or the galactic star lights. We also find that the observed gamma-ray luminosity is correlated better with the combined factor of the encounter rate and the background soft photon energy density. Therefore, the IC scattering may also contribute to the observed gamma-ray emission from GCs detected by Fermi in addition to the standard CR process. Furthermore, we find that the emission region of high-energy photons from GCs produced by the IC scattering is substantially larger than the cores of GCs with a radius >10 pc. The diffuse radio and X-rays emitted from GCs can also be produced by the synchrotron radiation and IC scattering, respectively. We suggest that future observations including radio, X-rays, and gamma rays

  9. Galactic evolution of sulphur as traced by globular clusters

    Science.gov (United States)

    Kacharov, N.; Koch, A.; Caffau, E.; Sbordone, L.

    2015-05-01

    Context. Sulphur is an important volatile α element, but its role in the Galactic chemical evolution is still uncertain, and more observations constraining the sulphur abundance in stellar photospheres are required. Aims: We derive the sulphur abundances in red giant branch (RGB) stars in three Galactic halo globular clusters (GC) that cover a wide metallicity range (-2.3 noise (S/N ~ 200 per px) spectra in the region of the S I multiplet 3 at 1045 nm for 15 GC stars selected from the literature (six stars in M 4,six stars in M 22, and three stars in M 30). Multiplet 3 is better suited for S abundance derivation than the more commonly used lines of multiplet 1 at 920 nm, since its lines are not blended by telluric absorption or other stellar features at low metallicity. Results: We used spectral synthesis to derive the [S/Fe] ratio of the stars assuming local thermodynamic equilibrium (LTE). We find mean [S/Fe]LTE = 0.58 ± 0.01 ± 0.20 dex (statistical and systematic error) for M 4, [S/Fe]LTE = 0.57 ± 0.01 ± 0.19 dex for M 22, and [S/Fe]LTE = 0.55 ± 0.02 ± 0.16 dex for M 30. The negative NLTE corrections are estimated to be in the order of the systematic uncertainties. We do not detect star-to-star variations of the S abundance in any of the observed GCs, with the possible exception of two individual stars, one in M 22 and one in M 30, which appear to be highly enriched in S. Conclusions: With the tentative exception of two stars with measured high S abundances, we conclude that sulphur behaves like a typical α element in the studied Galactic GCs, showing enhanced abundances with respect to the solar value at metallicities below [Fe/H]-1.0 dex without a considerable spread. Based on observations made with ESO telescopes at the La Silla Paranal Observatory under programmes ID 091.B-0171(A).The reduced spectra and the best fit synthetic models are available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc

  10. Systematic discovery of new recognition peptides mediating protein interaction networks

    DEFF Research Database (Denmark)

    Neduva, Victor; Linding, Rune; Su-Angrand, Isabelle

    2005-01-01

    Many aspects of cell signalling, trafficking, and targeting are governed by interactions between globular protein domains and short peptide segments. These domains often bind multiple peptides that share a common sequence pattern, or "linear motif" (e.g., SH3 binding to PxxP). Many domains...... by interactions between globular protein domains and short peptide segments. These domains often bind multiple peptides that share a common sequence pattern, or "linear motif" (e.g., SH3 binding to PxxP). Many domains are known, though comparatively few linear motifs have been discovered. Their short length...

  11. Depletion-induced instability in protein-DNA mixtures: Influence of protein charge and size

    NARCIS (Netherlands)

    Vries, de R.J.

    2006-01-01

    While there is abundant experimental and theoretical work on polymer-induced DNA condensation, it is still unclear whether globular proteins can condense linear DNA or not. We develop a simple analytical approximation for the depletion attraction between rodlike segments of semiflexible

  12. Electrorheological properties of suspensions of hollow globular titanium oxide/polypyrrole particles

    Czech Academy of Sciences Publication Activity Database

    Sedlačík, M.; Mrlík, M.; Pavlínek, V.; Sáha, P.; Quadrat, Otakar

    2012-01-01

    Roč. 290, č. 1 (2012), s. 41-48 ISSN 0303-402X R&D Projects: GA ČR GA202/09/1626 Institutional research plan: CEZ:AV0Z40500505 Keywords : electrorheology * titanium oxide * hollow globular clusters Subject RIV: JI - Composite Materials Impact factor: 2.161, year: 2012

  13. VLT/UVES abundances of individual stars in the Fornax dwarf spheroidal globular clusters

    NARCIS (Netherlands)

    Letarte, B.; Hill, V.; Jablonka, P.; Tolstoy, E.; Randich, S; Pasquini, L

    2006-01-01

    We present high resolution abundance analysis of nine stars belonging to three of the five globular clusters (GCs) of the Fornax dwarf galaxy. The spectra were taken with UVES at a resolution of 43 000. We find them to be slightly more metal-poor than what was previously calculated with other

  14. Integrated-light spectroscopy of globular clusters at the infrared Ca II lines

    Science.gov (United States)

    Armandroff, Taft E.; Zinn, Robert

    1988-01-01

    Integrated-light spectroscopy has been obtained for 27 globular clusters at the Ca II IR triplet. Line strengths and radial velocities have been measured from the spectra. For the well-studied clusters in the sample, the strength of the Ca II lines is very well correlated with previous metallicity estimates. Thus, the triplet is useful as a metallicity indicator in globular cluster integrated-light spectra. The greatly reduced effect of interstellar extinction at these wavelengths (compared to the blue region of the spectrum) has permitted observations of some of the most heavily reddened clusters in the Galaxy. For several such clusters, the Ca II triplet metallicities are in poor agreement with metallicity estimates from IR photometry by Malkan (1981). The strength of an interstellar band at 8621A has been used to estimate the amount of extinction towards these clusters. Using the new metallicity and radial-velocity data, the metallicity distribution, kinematics, and spatial distribution of the disk globular cluster system have been analyzed. Results very similar to those of Zinn (1985) have been found. The relation of the disk globulars to the stellar thick disk is discussed.

  15. On the blind use of statistical tools in the analysis of globular cluster stars

    Science.gov (United States)

    D'Antona, Francesca; Caloi, Vittoria; Tailo, Marco

    2018-04-01

    As with most data analysis methods, the Bayesian method must be handled with care. We show that its application to determine stellar evolution parameters within globular clusters can lead to paradoxical results if used without the necessary precautions. This is a cautionary tale on the use of statistical tools for big data analysis.

  16. Dynamics of globular molecules: moisture effect on the Rayleigh scattering spectrum of the Moessbauer radiation

    International Nuclear Information System (INIS)

    Chesskaya, T.Yu.

    1998-01-01

    The Rayleigh scattering spectrum of the Moessbauer radiation is plotted on the model simulating globular macromolecules. The modeling results are compared with experimental data on the spectra of the Rayleigh scattering of the Moessbauer radiation for various moisture content and hydratation dependence of the elastic scattering portion

  17. THE OBSERVATIONAL AND THEORETICAL TIDAL RADII OF GLOBULAR CLUSTERS IN M87

    International Nuclear Information System (INIS)

    Webb, Jeremy J.; Sills, Alison; Harris, William E.

    2012-01-01

    Globular clusters have linear sizes (tidal radii) which theory tells us are determined by their masses and by the gravitational potential of their host galaxy. To explore the relationship between observed and expected radii, we utilize the globular cluster population of the Virgo giant M87. Unusually deep, high signal-to-noise images of M87 are used to measure the effective and limiting radii of approximately 2000 globular clusters. To compare with these observations, we simulate a globular cluster population that has the same characteristics as the observed M87 cluster population. Placing these simulated clusters in the well-studied tidal field of M87, the orbit of each cluster is solved and the theoretical tidal radius of each cluster is determined. We compare the predicted relationship between cluster size and projected galactocentric distance to observations. We find that for an isotropic distribution of cluster velocities, theoretical tidal radii are approximately equal to observed limiting radii for R gc < 10 kpc. However, the isotropic simulation predicts a steep increase in cluster size at larger radii, which is not observed in large galaxies beyond the Milky Way. To minimize the discrepancy between theory and observations, we explore the effects of orbital anisotropy on cluster sizes, and suggest a possible orbital anisotropy profile for M87 which yields a better match between theory and observations. Finally, we suggest future studies which will establish a stronger link between theoretical tidal radii and observed radii.

  18. Photoelectric UBVRI sequences in the Galactic globular clusters NGC 6752 and NGC 6864

    International Nuclear Information System (INIS)

    Alvarado, F.; Wenderoth, E.; Alcaino, G.; Liller, W.

    1990-01-01

    UBVRI photoelectric sequences for the Galactic globular clusters NGC 6752 and NGC 6864 are presented. Both of them include fields suitable for CCD exposures. From five UBV sequences in NGC 6572, only five stars are in common with the previous works. 15 refs

  19. A Fossil Bulge Globular Cluster Revealed by very Large Telescope Multi-conjugate Adaptive Optics

    Czech Academy of Sciences Publication Activity Database

    Ortolani, S.; Barbuy, B.; Momany, Y.; Saviane, I.; Bica, E.; Jílková, L.; Salerno, G.M.; Jungwiert, Bruno

    2011-01-01

    Roč. 737, č. 1 (2011), 31/1-31/9 ISSN 0004-637X Institutional research plan: CEZ:AV0Z10030501 Keywords : galaxy * globular clusters Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 6.024, year: 2011

  20. The Case of the Missing Cyanogen-rich AGB Stars in Galactic Globular Clusters

    DEFF Research Database (Denmark)

    Campbell, S. W.; Yong, D.; Wylie-de Boer, E. C.

    2012-01-01

    The handful of available observations of AGB stars in Galactic Globular Clusters suggest that the GC AGB populations are dominated by cyanogen-weak stars (eg. Norris et al. 1981; Sneden et al. 2000). This contrasts strongly with the distributions on the RGB (and other) populations, which generall...

  1. The deposition of globular polypyrrole and polypyrrole nanotubes on cotton textile

    Czech Academy of Sciences Publication Activity Database

    Bober, Patrycja; Stejskal, Jaroslav; Šeděnková, Ivana; Trchová, Miroslava; Martinková, L.; Marek, J.

    2015-01-01

    Roč. 356, 30 November (2015), s. 737-741 ISSN 0169-4332 R&D Projects: GA TA ČR(CZ) TE01020022 Institutional support: RVO:61389013 Keywords : conducting textile * cotton * globular polypyrrole Subject RIV: CG - Electrochemistry Impact factor: 3.150, year: 2015

  2. Curve-of-growth analysis of a red giant in the globular cluster M13

    International Nuclear Information System (INIS)

    Griffin, R.

    1979-01-01

    A coude spectrogram of a red giant (L973) in the globular cluster M13 is analysed, with respect to α Boo, by the differential curve-of-growth technique. The overall metal abundance is found to be approximately one-tenth of that of α Boo, or one-fortieth that of the Sun. (author)

  3. Three Ancient Halo Subgiants: Precise Parallaxes, Compositions, Ages, and Implications for Globular Clusters

    DEFF Research Database (Denmark)

    VandenBerg, Don A.; Bond, Howard E.; Nelan, Edmund P.

    2014-01-01

    very soon after the Big Bang. (Stellar models that neglect diffusive processes seem to be ruled out as they would predict that HD 140283 is ~1.5 Gyr older than the universe.) The field halo subgiants appear to be older than globular clusters of similar metallicities: if distances close to those implied...

  4. Dating the Tidal Disruption of Globular Clusters with GAIA Data on Their Stellar Streams

    Science.gov (United States)

    Bose, Sownak; Ginsburg, Idan; Loeb, Abraham

    2018-05-01

    The Gaia mission promises to deliver precision astrometry at an unprecedented level, heralding a new era for discerning the kinematic and spatial coordinates of stars in our Galaxy. Here, we present a new technique for estimating the age of tidally disrupted globular cluster streams using the proper motions and parallaxes of tracer stars. We evolve the collisional dynamics of globular clusters within the evolving potential of a Milky Way-like halo extracted from a cosmological ΛCDM simulation and analyze the resultant streams as they would be observed by Gaia. The simulations sample a variety of globular cluster orbits, and account for stellar evolution and the gravitational influence of the disk of the Milky Way. We show that a characteristic timescale, obtained from the dispersion of the proper motions and parallaxes of stars within the stream, is a good indicator for the time elapsed since the stream has been freely expanding away due to the tidal disruption of the globular cluster. This timescale, in turn, places a lower limit on the age of the cluster. The age can be deduced from astrometry using a modest number of stars, with the error on this estimate depending on the proximity of the stream and the number of tracer stars used.

  5. The SLUGGS survey: globular cluster stellar population trends from weak absorption lines in stacked spectra

    Science.gov (United States)

    Usher, Christopher; Forbes, Duncan A.; Brodie, Jean P.; Romanowsky, Aaron J.; Strader, Jay; Conroy, Charlie; Foster, Caroline; Pastorello, Nicola; Pota, Vincenzo; Arnold, Jacob A.

    2015-01-01

    As part of the SAGES Legacy Unifying Globulars and GalaxieS (SLUGGS) survey, we stack 1137 Keck DEIMOS (Deep Imaging Multi-Object Spectrograph) spectra of globular clusters from 10 galaxies to study their stellar populations in detail. The stacked spectra have median signal-to-noise ratios of ˜90 Å-1. Besides the calcium triplet, we study weaker sodium, magnesium, titanium and iron lines as well as the Hα and higher order Paschen hydrogen lines. In general, the stacked spectra are consistent with old ages and a Milky Way-like initial mass function. However, we see different metal line index strengths at fixed colour and magnitude, and differences in the calcium triplet-colour relation from galaxy to galaxy. We interpret this as strong evidence for variations in the globular cluster colour-metallicity relation between galaxies. Two possible explanations for the colour-metallicity relation variations are that the average ages of globular clusters vary from galaxy to galaxy or that the average abundances of light elements (i.e. He, C, N and O) differ between galaxies. Stacking spectra by magnitude, we see that the colours become redder and metal line indices stronger with brighter magnitudes. These trends are consistent with the previously reported `blue tilts' being mass-metallicity relations.

  6. The Observational and Theoretical Tidal Radii of Globular Clusters in M87

    Science.gov (United States)

    Webb, Jeremy J.; Sills, Alison; Harris, William E.

    2012-02-01

    Globular clusters have linear sizes (tidal radii) which theory tells us are determined by their masses and by the gravitational potential of their host galaxy. To explore the relationship between observed and expected radii, we utilize the globular cluster population of the Virgo giant M87. Unusually deep, high signal-to-noise images of M87 are used to measure the effective and limiting radii of approximately 2000 globular clusters. To compare with these observations, we simulate a globular cluster population that has the same characteristics as the observed M87 cluster population. Placing these simulated clusters in the well-studied tidal field of M87, the orbit of each cluster is solved and the theoretical tidal radius of each cluster is determined. We compare the predicted relationship between cluster size and projected galactocentric distance to observations. We find that for an isotropic distribution of cluster velocities, theoretical tidal radii are approximately equal to observed limiting radii for R gc < 10 kpc. However, the isotropic simulation predicts a steep increase in cluster size at larger radii, which is not observed in large galaxies beyond the Milky Way. To minimize the discrepancy between theory and observations, we explore the effects of orbital anisotropy on cluster sizes, and suggest a possible orbital anisotropy profile for M87 which yields a better match between theory and observations. Finally, we suggest future studies which will establish a stronger link between theoretical tidal radii and observed radii.

  7. La galaxia NGC 6876 y su sistema de cúmulos globulares

    Science.gov (United States)

    Ennis, A. I.; Bassino, L. P.; Caso, J. P.

    2017-10-01

    We present preliminary results of the deep photometric study of the elliptical galaxy NGC6876, located at the center of the Pavo group, and its globular cluster system. We use images obtained with the GMOS camera mounted on the Gemini South telescope, in the and bands, with the purpose of disentangling the evolutionary history of the galaxy on the basis of their characteristics.

  8. Multiple populations along the asymptotic giant branch of the globular cluster M 4

    NARCIS (Netherlands)

    Lardo, C.; Salaris, M.; Savino, A.; Donati, P.; Stetson, P. B.; Cassisi, S.

    2016-01-01

    Nearly all Galactic globular clusters host stars that display characteristic abundance anti-correlations, like the O-rich/Na-poor pattern typical of field halo stars, together with O-poor/Na-rich additional components. A recent spectroscopic investigation questioned the presence of O-poor/Na-rich

  9. Disrupted globular clusters and the gamma-ray excess in the Galactic Centre

    Science.gov (United States)

    Fragione, Giacomo; Antonini, Fabio; Gnedin, Oleg Y.

    2018-04-01

    The Fermi Large Area Telescope has provided the most detailed view towards the Galactic Centre (GC) in high-energy gamma-rays. Besides the interstellar emission and point source contributions, the data suggest a residual diffuse gamma-ray excess. The similarity of its spatial distribution with the expected profile of dark matter has led to claims that this may be evidence for dark matter particle annihilation. Here, we investigate an alternative explanation that the signal originates from millisecond pulsars (MSPs) formed in dense globular clusters and deposited at the GC as a consequence of cluster inspiral and tidal disruption. We use a semi-analytical model to calculate the formation, migration, and disruption of globular clusters in the Galaxy. Our model reproduces the mass of the nuclear star cluster and the present-day radial and mass distribution of globular clusters. For the first time, we calculate the evolution of MSPs from disrupted globular clusters throughout the age of the Galaxy and consistently include the effect of the MSP spin-down due to magnetic-dipole braking. The final gamma-ray amplitude and spatial distribution are in good agreement with the Fermi observations and provide a natural astrophysical explanation for the GC excess.

  10. Search for continuous gravitational waves from neutron stars in globular cluster NGC 6544

    NARCIS (Netherlands)

    Abbott, B. P.; Abbott, R.; Abbott, T. D.; Abernathy, M. R.; Acernese, F.; Ackley, K.; Adams, C.; Phythian-Adams, A.T.; Addesso, P.; Adhikari, R. X.; Adya, V. B.; Affeldt, C.; Agathos, M.; Agatsuma, K.; Aggarwal, N.T.; Aguiar, O. D.; Aiello, L.; Ain, A.; Allen, B.; Allocca, A.; Altin, P. A.; Anderson, S. B.; Anderson, W. G.; Arai, K.; Araya, M. C.; Arceneaux, C. C.; Areeda, J. S.; Arnaud, N.; Arun, K. G.; Ascenzi, S.; Ashton, G.; Ast, M.; Aston, S. M.; Astone, P.; Aufmuth, P.; Aulbert, C.; Babak, S.; Bacon, P.; Bader, M. K. M.; Baker, P. T.; Baldaccini, F.; Ballardin, G.; Ballmer, S. W.; Barayoga, J. C.; Barclay, S. E.; Barish, B. C.; Barker, R.D.; Barone, F.; Barr, B.; Barsotti, L.; Barsuglia, M.; Barta, D.; Bartlett, J.; Bartos, I.; Bassiri, R.; Basti, A.; Batch, J. C.; Baune, C.; Bavigadda, V.; Bazzan, M.; Bejger, M.; Bell, A. S.; Berger, B. K.; Bergmann, G.; Berry, C. P. L.; Bersanetti, D.; Bertolini, A.; Betzwieser, J.; Bhagwat, S.; Bhandare, R.; Bilenko, I. A.; Billingsley, G.; Birch, M.J.; Birney, R.; Biscans, S.; Bisht, A.; Bitossi, M.; Biwer, C.; Bizouard, M. A.; Blackburn, J. K.; Blair, C. D.; Blair, D. G.; Blair, R. M.; Bloemen, A.L.S.; Bock, O.; Boer, M.; Bogaert, J.G.; Bogan, C.; Bohe, A.; Bond, T.C; Bondu, F.; Bonnand, R.; Boom, B. A.; Bork, R.; Boschi, V.; Bose, S.; Bouffanais, Y.; Bozzi, A.; Bradaschia, C.; Brady, P. R.; Braginsky, V. B.; Branchesi, M.; Brau, J. E.; Briant, T.; Brillet, A.; Brinkmann, M.; Brisson, V.; Brockill, P.; Broida, J. E.; Brooks, A. F.; Brown, A.D.; Brown, D.; Brown, N. M.; Brunett, S.; Buchanan, C. C.; Buikema, A.; Bulik, T.; Bulten, H. J.; Buonanno, A.; Buskulic, D.; Buy, C.; Byer, R. L.; Cabero, M.; Cadonati, L.; Cagnoli, G.; Cahillane, C.; Bustillo, J. Calderon; Callister, T. A.; Calloni, E.; Camp, J. B.; Cannon, K. C.; Cao, J.; Capano, C. D.; Capocasa, E.; Carbognani, F.; Caride, S.; Diaz, J. Casanueva; Casentini, C.; Caudill, S.; Cavaglia, M.; Cavalier, F.; Cavalieri, R.; Cella, G.; Cepeda, C. B.; Baiardi, L. Cerboni; Cerretani, G.; Cesarini, E.; Chamberlin, S. J.; Chan, M.; Chao, D. S.; Charlton, P.; Chassande-Mottin, E.; Cheeseboro, B. D.; Chen, H. Y.; Chen, Y; Cheng, C.; Chincarini, A.; Chiummo, A.; Cho, H. S.; Cho, M.; Chow, J. H.; Christensen, N.; Chu, Qian; Chua, S. S. Y.; Chung, E.S.; Ciani, G.; Clara, F.; Clark, J. A.; Cleva, F.; Coccia, E.; Cohadon, P. -F.; Colla, A.; Collette, C. G.; Cominsky, L.; Constancio, M., Jr.; Conte, A.; Conti, L.; Cook, D.; Corbitt, T. R.; Cornish, N.; Corsi, A.; Cortese, S.; Costa, A.C.; Coughlin, M. W.; Coughlin, S. B.; Coulon, J. -P.; Countryman, S. T.; Couvares, P.; Cowan, E. E.; Coward, D. M.; Cowart, M. J.; Coyne, D. C.; Coyne, R.; Craig, K.; Creighton, J. D. E.; Creighton, T. D.; Cripe, J.; Crowder, S. G.; Cumming, A.; Cunningham, Laura; Cuoco, E.; Dal Canton, T.; Danilishin, S. L.; D'Antonio, S.; Danzmann, K.; Darman, N. S.; Dasgupta, A.; Costa, C. F. Da Silva; Dattilo, V.; Dave, I.; Davier, M.; Davies, G. S.; Daw, E. J.; Day, R.; De, S.; Debra, D.; Debreczeni, G.; Degallaix, J.; De laurentis, M.; Deleglise, S.; Del Pozzo, W.; Denker, T.; Dent, T.; Dergachev, V.A.; Rosa, R.; DeRosa, R. T.; DeSalvo, R.; Devine, R. C.; Dhurandhar, S.; Diaz, M. C.; Di Fiore, L.; Giovanni, M. Di; Di Girolamo, T.; Di Lieto, A.; Di Pace, S.; Di Palma, I.; Di Virgilio, A.; Dolique, V.; Donovan, F.; Dooley, K. L.; Doravari, S.; Douglas, R.; Downes, T. P.; Drago, M.; Drever, R. W. P.; Driggers, J. C.; Ducrot, M.; Dwyer, S. E.; Edo, T. B.; Edwards, M. C.; Effler, A.; Eggenstein, H. -B.; Ehrens, P.; Eichholz, J.; Eikenberry, S. S.; Engels, W.; Essick, R. C.; Etzel, T.; Evans, T. M.; Evans, T. M.; Everett, R.; Factourovich, M.; Fafone, V.; Fair, H.; Fan, X.M.; Fang, Q.; Farinon, S.; Farr, B.; Farr, W. M.; Favata, M.; Fays, M.; Fehrmann, H.; Fejer, M. M.; Fenyvesi, E.; Ferrante, I.; Ferreira, E. C.; Ferrini, F.; Fidecaro, F.; Fiori, I.; Fiorucci, D.; Fisher, R. P.; Flaminio, R.; Fletcher, M; Fournier, J. -D.; Frasca, S.; Frasconi, F.; Frei, Z.; Freise, A.; Frey, R.; Frey, V.; Fritschel, P.; Frolov, V. V.; Fulda, P.; Fyffe, M.; Gabbard, H. A. G.; Gair, J. R.; Gammaitoni, L.; Gaonkar, S. G.; Garufi, F.; Gaur, G.; Gehrels, N.; Gemme, G.; Geng, P.; Genin, E.; Gennai, A.; George, J.; Gergely, L.; Germain, V.; Ghosh, Abhirup; Ghosh, Archisman; Ghosh, S.; Giaime, J. A.; Giardina, K. D.; Giazotto, A.; Gill, K.P.; Glaefke, A.; Goetz, E.; Goetz, R.; Gondan, L.; Gonzalez, Idelmis G.; Castro, J. M. Gonzalez; Gopakumar, A.; Gordon, N. A.; Gorodetsky, M. L.; Gossan, S. E.; Lee-Gosselin, M.; Gouaty, R.; Grado, A.; Graef, C.; Graff, P. B.; Granata, M.; Grant, A.; Gras, S.; Gray, C.M.; Greco, G.; Green, A. C.; Groot, P.; Grote, H.; Grunewald, S.; Guidi, G. M.; Guo, X.; Gupta, A.; Gupta, M. K.; Gushwa, K. E.; Gustafson, E. K.; Gustafson, R.; Hacker, J. J.; Buffoni-Hall, R.; Hall, E. D.; Hammond, G.L.; Haney, M.; Hanke, M. M.; Hanks, J.; Hanna, C.; Hanson, P.J.; Hardwick, T.; Harms, J.; Harry, G. M.; Harry, I. W.; Hart, M. J.; Hartman, M. T.; Haster, C. -J.; Haughian, K.; Heidmann, A.; Heintze, M. C.; Heitmann, H.; Hello, P.; Hemming, G.; Hendry, M.; Heng, I. S.; Hennig, J.; Henry, J.A.; Heptonstall, A. W.; Heurs, M.; Hild, S.; Hoak, D.; Hofman, D.; Holt, K.; Holz, D. E.; Hopkins, P.; Hough, J.; Houston, E. A.; Howell, E. J.; Hu, Y. M.; Huang, S.; Huerta, E. A.; Huet, D.; Hughey, B.; Husa, S.; Huttner, S. H.; Huynh-Dinh, T.; Indik, N.; Ingram, D. R.; Inta, R.; Isa, H. N.; Isac, J. -M.; Isi, M.; Isogai, T.; Iyer, B. R.; Izumi, K.; Jacqmin, T.; Jang, D.H.; Jani, K.; Jaranowski, P.; Jawahar, S.; Jian, L.; Jimenez-Forteza, F.; Johnson, W.; Jones, I.D.; Jones, R.; Jonker, R. J. G.; Ju, L.; Haris, K.; Kalaghatgi, C. V.; Kalogera, V.; Kandhasamy, S.; Kang, G.H.; Kanner, J. B.; Kapadia, S. J.; Karki, S.; Karvinen, K. S.; Kasprzack, M.; Katsavounidis, E.; Katzman, W.; Kaufer, S.; Kaur, T.; Kawabe, K.; Kefelian, F.; Kehl, M. S.; Keitel, D.; Kelley, D. B.; Kells, W.; Kennedy, R.E.; Key, J. S.; Khalili, F. Y.; Khan, I.; Khan., S.; Khan, Z.; Khazanov, E. A.; Kijbunchoo, N.; Kim, Chi-Woong; Kim, Chunglee; Kim, J.; Kim, K.; Kim, Namjun; Kim, W.; Kim, Y.M.; Kimbrell, S. J.; King, E. J.; King, P. J.; Kissel, J. S.; Klein, B.; Kleybolte, L.; Klimenko, S.; Koehlenbeck, S. M.; Koley, S.; Kondrashov, V.; Kontos, A.; Korobko, M.; Korth, W. Z.; Kowalska, I.; Kozak, D. B.; Kringel, V.; Krishnan, B.; Krolak, A.; Krueger, C.; Kuehn, G.; Kumar, P.; Kumar, R.; Kuo, L.; Kutynia, A.; Lackey, B. D.; Landry, M.; Lange, J.; Lantz, B.; Lasky, P. D.; Laxen, M.; Lazzaro, C.; Leaci, P.; Leavey, S.; Lebigot, E. O.; Lee, C.H.; Lee, K.H.; Lee, M.H.; Lee, K.; Lenon, A.; Leonardi, M.; Leong, J. R.; Leroy, N.; Letendre, N.; Levin, Y.; Lewis, J. B.; Li, T. G. F.; Libson, A.; Littenberg, T. B.; Lockerbie, N. A.; Lombardi, A. L.; London, L. T.; Lord, J. E.; Lorenzini, M.; Loriette, V.; Lormand, M.; Losurdo, G.; Lough, J. D.; Lueck, H.; Lundgren, A. P.; Lynch, R.; Ma, Y.; Machenschalk, B.; MacInnis, M.; Macleod, D. M.; Magana-Sandoval, F.; Zertuche, L. Magana; Magee, R. M.; Majorana, E.; Maksimovic, I.; Malvezzi, V.; Man, N.; Mandic, V.; Mangano, V.; Mansell, G. L.; Manske, M.; Mantovani, M.; Marchesoni, F.; Marion, F.; Marka, S.; Marka, Z.; Markosyan, A. S.; Maros, E.; Martelli, F.; Martellini, L.; Martin, I. W.; Martynov, D. V.; Marx, J. N.; Mason, K.; Masserot, A.; Massinger, T. J.; Masso-Reid, M.; Mastrogiovanni, S.; Matichard, F.; Matone, L.; Mavalvala, N.; Mazumder, N.; McCarthy, R.; McClelland, D. E.; McCormick, S.; McGuire, S. C.; McIntyre, G.; McIver, J.; McManus, D. J.; McRae, T.; McWilliams, S. T.; Meacher, D.; Meadors, G. D.; Meidam, J.; Melatos, A.; Mendell, G.; Mercer, R. A.; Merilh, E. L.; Merzougui, M.; Meshkov, S.; Messenger, C.; Messick, C.; Metzdorff, R.; Meyers, P. M.; Mezzani, F.; Miao, H.; Michel, C.; Middleton, H.; Mikhailov, E. E.; Milano, L.; Miller, A. L.; Miller, A. L.; Miller, B.; Miller, J.; Millhouse, M.; Minenkov, Y.; Ming, J.; Mirshekari, S.; Mishra, C.; Mitra, S.; Mitrofanov, V. P.; Mitselmakher, G.; Mittleman, R.; Moggi, A.; Mohan, M.; Mohapatra, S. R. P.; Montani, M.; Moore, B.C.; Moore, J.C.; Moraru, D.; Gutierrez Moreno, M.; Morriss, S. R.; Mossavi, K.; Mours, B.; Mow-Lowry, C. M.; Mueller, G.; Muir, A. W.; Mukherjee, Arunava; Mukherjee, S.D.; Mukherjee, S.; Mukund, N.; Mullavey, A.; Munch, J.; Murphy, D. J.; Murray, P.G.; Mytidis, A.; Nardecchia, I.; Naticchioni, L.; Nayak, R. K.; Nedkova, K.; Nelemans, G.; Nelson, T. J. N.; Gutierrez-Neri, M.; Neunzert, A.; Newton-Howes, G.; Nguyen, T. T.; Nielsen, A. B.; Nissanke, S.; Nitz, A.; Nocera, F.; Nolting, D.; Normandin, M. E. N.; Nuttall, L. K.; Oberling, J.; Ochsner, E.; O'Dell, J.; Oelker, E.; Ogin, G. H.; Oh, J.; Oh, S. H.; Ohme, F.; Oliver, M. B.; Oppermann, P.; Oram, Richard J.; O'Reilly, B.; O'Shaughnessy, R.; Ottaway, D. J.; Overmier, H.; Owen, B. J.; Pai, A.; Pai, S. A.; Palamos, J. R.; Palashov, O.; Palomba, C.; Pal-Singh, A.; Pan, H.; Pankow, C.; Pannarale, F.; Pant, B. C.; Paoletti, F.; Paoli, A.; Papa, M. A.; Paris, H. R.; Parker, W.S; Pascucci, D.; Pasqualetti, A.; Passaquieti, R.; Passuello, D.; Patel, P.; Patricelli, B.; Patrick, Z.; Pearlstone, B. L.; Pedraza, M.; Pedurand, R.; Pekowsky, L.; Pele, A.; Penn, S.; Perreca, A.; Perri, L. M.; Phelps, M.; Piccinni, O. J.; Pichot, M.; Piergiovanni, F.; Pierro, V.; Pillant, G.; Pinard, L.; Pinto, I. M.; Pitkin, M.; Poe, M.; Poggiani, R.; Popolizio, P.; Post, A.; Powell, J.; Prasad, J.; Predoi, V.; Prestegard, T.; Price, L. R.; Prijatelj, M.; Principe, M.; Privitera, S.; Prix, R.; Prodi, G. A.; Prokhorov, L. G.; Puncken, O.; Punturo, M.; Puppo, P.; Puerrer, M.; Qi, H.; Qin, J.; Qiu, S.; Quetschke, V.; Quintero, E. A.; Quitzow-James, R.; Raab, F. J.; Rabeling, D. S.; Radkins, H.; Raffai, P.; Raja, S.; Rajan, C.; Rakhmanov, M.; Rapagnani, P.; Raymond, V.; Razzano, M.; Re, V.; Read, J.; Reed, C. M.; Regimbau, T.; Rei, L.; Reid, S.; Reitze, D. H.; Rew, H.; Reyes, S. D.; Ricci, F.; Riles, K.; Rizzo, D.M.; Robertson, N. A.; Robie, R.; Robinet, F.; Rocchi, A.; Rolland, L.; Rollins, J. G.; Roma, V. J.; Romano, R.; Romanov, G.; Romie, J. H.; Rosinska, D.; Rowan, S.; Ruediger, A.; Ruggi, P.; Ryan, K.A.; Sachdev, Perminder S; Sadecki, T.; Sadeghian, L.; Sakellariadou, M.; Salconi, L.; Saleem, M.; Salemi, F.; Samajdar, A.; Sammut, L.; Sanchez, E. J.; Sandberg, V.; Sandeen, B.; Sanders, J. R.; Sassolas, B.; Saulson, P. R.; Sauter, O. E. S.; Savage, R. L.; Sawadsky, A.; Schale, P.; Schilling, R.; Schmidt, J; Schmidt, P.; Schnabel, R.B.; Schofield, R. M. S.; Schoebeck, A.; Schreiber, K.E.C.; Schuette, D.; Schutz, B. F.; Scott, J.; Scott, M.S.; Sellers, D.; Sengupta, A. S.; Sentenac, D.; Sequino, V.; Sergeev, A.; Setyawati, Y.; Shaddock, D. A.; Shaffer, T. J.; Shahriar, M. S.; Shaltev, M.; Shapiro, B.; Shawhan, P.; Sheperd, A.; Shoemaker, D. H.; Shoemaker, D. M.; Siellez, K.; Siemens, X.; Sieniawska, M.; Sigg, D.; Silva, António Dias da; Singer, A; Singer, L. P.; Singh, A.; Singh, R.; Singhal, A.; Sintes, A. M.; Slagmolen, B. J. J.; Smith, R. J. E.; Smith, N.D.; Smith, R. J. E.; Son, E. J.; Sorazu, B.; Sorrentino, F.; Souradeep, T.; Srivastava, A. K.; Staley, A.; Steinke, M.; Steinlechner, J.; Steinlechner, S.; Steinmeyer, D.; Stephens, B. C.; Stone, J.R.; Strain, K. A.; Straniero, N.; Stratta, G.; Strauss, N. A.; Strigin, S. E.; Sturani, R.; Stuver, A. L.; Summerscales, T. Z.; Sun, L.; Sunil, S.; Sutton, P. J.; Swinkels, B. L.; Szczepanczyk, M. J.; Tacca, M.D.; Talukder, D.; Tanner, D. B.; Tapai, M.; Tarabrin, S. P.; Taracchini, A.; Taylor, W.R.; Theeg, T.; Thirugnanasambandam, M. P.; Thomas, E. G.; Thomas, M.; Thomas, P.; Thorne, K. A.; Thrane, E.; Tiwari, S.; Tiwari, V.; Tokmakov, K. V.; Toland, K.; Tomlinson, C.; Tonelli, M.; Tornasi, Z.; Torres, C. V.; Torrie, C. I.; Toyra, D.; Travasso, F.; Traylor, G.; Trifiro, D.; Tringali, M. C.; Trozzo, L.; Tse, M.; Turconi, M.; Tuyenbayev, D.; Ugolini, D.; Unnikrishnan, C. S.; Urban, A. L.; Usman, S. A.; Vahlbruch, H.; Vajente, G.; Valdes, G.; van Bakel, N.; Van Beuzekom, Martin; van den Brand, J. F. J.; Van Den Broeck, C.F.F.; Vander-Hyde, D. C.; van der Schaaf, L.; van Heijningen, J. V.; van Veggel, A. A.; Vardaro, M.; Vass, S.; Vasuth, M.; Vaulin, R.; Vecchio, A.; Vedovato, G.; Veitch, J.; Veitch, P.J.; Venkateswara, K.; Verkindt, D.; Vetrano, F.; Vicere, A.; Vinciguerra, S.; Vine, D. J.; Vinet, J. -Y.; Vitale, S.; Vo, T.; Vocca, H.; Vorvick, C.; Voss, D. V.; Vousden, W. D.; Vyatchanin, S. P.; Wade, A. R.; Wade, L. E.; Wade, MT; Walker, M.; Wallace, L.; Walsh, S.; Wang, G.; Wang, H.; Wang, M.; Wang, X.; Wang, Y.; Ward, R. L.; Warner, J.; Was, M.; Weaver, B.; Wei, L. -W.; Weinert, M.; Weinstein, A. J.; Weiss, R.; Wen, L.M.; Wessels, P.; Westphal, T.; Wette, K.; Whelan, J. T.; Whiting, B. F.; Williams, D.R.; Williamson, A. R.; Willis, J. L.; Willke, B.; Wimmer, M. H.; Winkler, W.; Wipf, C. C.; Wittel, H.; Woan, G.; Woehler, J.; Worden, J.; Wright, J.L.; Wu, D.S.; Wu, G.; Yablon, J.; Yam, W.; Yamamoto, H.; Yancey, C. C.; Yu, H.; Yvert, M.; Zadrozny, A.; Zangrando, L.; Zanolin, M.; Zendri, J. -P.; Zevin, M.; Zhang, L.; Zhang, M.; Zhang, Y.; Zhao, C.; Zhou, M.; Zhou, Z.; Zhu, X. J.; Zucker, M. E.; Zuraw, S. E.; Zweizig, J.; Sigurdsson, S.

    2017-01-01

    We describe a directed search for continuous gravitational waves in data from the sixth initial LIGO science run. The target was the nearby globular cluster NGC 6544 at a distance of approximate to 2.7 kpc. The search covered a broad band of frequencies along with first and second frequency

  11. Cloning and expression of adiponectin and its globular domain, and measurement of the biological activity in vivo.

    Science.gov (United States)

    Hu, Xiao-Bo; Zhang, Yu-Jian; Zhang, Hui-Tang; Yang, Sheng-Li; Gong, Yi

    2003-11-01

    3T3-L1-adipocytes produce the adipocyte complement related protein of 30 kD (ACRP30), which is exclusively expressed in differentiated adipocytes. Decreased expression of ACRP30 correlates with insulin resistance in mouse models of altered insulin sensitivity. Adiponectin, the human homologue of ACRP30, circulates in human plasma at high levels. Plasma adiponectin levels have been reported to be decreased in some insulin-resistant states, such as obesity and type II diabetes mellitus. Here, full-length adiponectin and its C-terminal globular head domain (gAdiponectin) were expressed in Escherichia coli and gAdiponectin was used to immunize a rabbit to obtain polyclonal antiserum with titer of 10,000. Adiponectin was detected in human plasma with the use of gAdiponectin anti-serum by Western blot analysis, which was also detected by gACRP30 anti-serum. Injection in alloxan-treated rats with purified recombinant fusion adiponectin or gAdiponectin transiently abolished hyperglycemia. So adiponectin and gAdiponectin might have activity as a glucose lowering agent and potentially as a therapeutic for metabolic disease. All these results suggested that the recombinant protein had biological activity, and provided a useful tool in further studies.

  12. Electrophoretic characterization of protein interactions suggesting limited feasibility of accelerated shelf-life testing of ultra-high temperature milk.

    Science.gov (United States)

    Grewal, Manpreet Kaur; Chandrapala, Jayani; Donkor, Osaana; Apostolopoulos, Vasso; Vasiljevic, Todor

    2017-01-01

    Accelerated shelf-life testing is applied to a variety of products to estimate keeping quality over a short period of time. The industry has not been successful in applying this approach to ultra-high temperature (UHT) milk because of chemical and physical changes in the milk proteins that take place during processing and storage. We investigated these protein changes, applying accelerated shelf-life principles to UHT milk samples with different fat levels and using native- and sodium dodecyl sulfate-PAGE. Samples of UHT skim and whole milk were stored at 20, 30, 40, and 50°C for 28d. Irrespective of fat content, UHT treatment had a similar effect on the electrophoretic patterns of milk proteins. At the start of testing, proteins were bonded mainly through disulfide and noncovalent interactions. However, storage at and above 30°C enhanced protein aggregation via covalent interactions. The extent of aggregation appeared to be influenced by fat content; whole milk contained more fat than skim milk, implying aggregation via melted or oxidized fat, or both. Based on reduction in loss in absolute quantity of individual proteins, covalent crosslinking in whole milk was facilitated mainly by products of lipid oxidation and increased access to caseins for crosslinking reactions. Maillard and dehydroalanine products were the main contributors involved in protein changes in skim milk. Protein crosslinking appeared to follow a different pathway at higher temperatures (≥40°C) than at lower temperatures, making it very difficult to extrapolate these changes to protein interactions at lower temperatures. Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  13. Changes in protein expression in testes of L2 strain Taiwan country chickens in response to acute heat stress.

    Science.gov (United States)

    Wang, Shih-Han; Cheng, Chuen-Yu; Chen, Chao-Jung; Chen, Hsin-Hsin; Tang, Pin-Chi; Chen, Chih-Feng; Lee, Yen-Pai; Huang, San-Yuan

    2014-07-01

    Heat stress causes a decrease of fertility in roosters. Yet, the way acute heat stress affects protein expression remains poorly understood. This study investigated differential protein expression in testes of the L2 strain of Taiwan country chickens following acute heat stress. Twelve 45-week-old roosters were allocated into four groups, including control roosters kept at 25 °C, roosters subjected to 38 °C acute heat stress for 4 hours without recovery, with 2 hours of recovery, and with 6 hours of recovery. Testis samples were collected for morphologic assay and protein analysis. Some of the differentially expressed proteins were validated by Western blot and immunohistochemistry. Abnormal and apoptotic spermatogenic cells were observed at 2 hours of recovery after acute heat stress, especially among the spermatocytes. Two-dimensional difference gel electrophoresis revealed that 119 protein spots were differentially expressed in chicken testes following heat stress, and peptide mass fingerprinting revealed that these spots contained 92 distinct proteins. In the heat-stressed samples, the heat shock proteins, chaperonin containing t-complex, and proteasome subunits were downregulated, and glutathione S-transferase, transgelin, and DJ-1 were upregulated. Our results demonstrate that acute heat stress impairs the processes of translation, protein folding, and protein degradation, and thus results in apoptosis and interferes with spermatogenesis. On the other hand, the increased expression of antioxidant enzymes, including glutathione S-transferase and DJ-1, may attenuate heat-induced damage. These findings may have implications for breeding chickens that can tolerate more extreme conditions. Copyright © 2014 Elsevier Inc. All rights reserved.

  14. Modeling the formation of globular cluster systems in the Virgo cluster

    International Nuclear Information System (INIS)

    Li, Hui; Gnedin, Oleg Y.

    2014-01-01

    The mass distribution and chemical composition of globular cluster (GC) systems preserve fossil record of the early stages of galaxy formation. The observed distribution of GC colors within massive early-type galaxies in the ACS Virgo Cluster Survey (ACSVCS) reveals a multi-modal shape, which likely corresponds to a multi-modal metallicity distribution. We present a simple model for the formation and disruption of GCs that aims to match the ACSVCS data. This model tests the hypothesis that GCs are formed during major mergers of gas-rich galaxies and inherit the metallicity of their hosts. To trace merger events, we use halo merger trees extracted from a large cosmological N-body simulation. We select 20 halos in the mass range of 2 × 10 12 to 7 × 10 13 M ☉ and match them to 19 Virgo galaxies with K-band luminosity between 3 × 10 10 and 3 × 10 11 L ☉ . To set the [Fe/H] abundances, we use an empirical galaxy mass-metallicity relation. We find that a minimal merger ratio of 1:3 best matches the observed cluster metallicity distribution. A characteristic bimodal shape appears because metal-rich GCs are produced by late mergers between massive halos, while metal-poor GCs are produced by collective merger activities of less massive hosts at early times. The model outcome is robust to alternative prescriptions for cluster formation rate throughout cosmic time, but a gradual evolution of the mass-metallicity relation with redshift appears to be necessary to match the observed cluster metallicities. We also affirm the age-metallicity relation, predicted by an earlier model, in which metal-rich clusters are systematically several billion younger than their metal-poor counterparts.

  15. RADIAL VELOCITIES FROM VLT-KMOS SPECTRA OF GIANT STARS IN THE GLOBULAR CLUSTER NGC 6388

    International Nuclear Information System (INIS)

    Lapenna, E.; Mucciarelli, A.; Lanzoni, B.; Ferraro, F. R.; Dalessandro, E.; Origlia, L.; Valenti, E.; Cirasuolo, M.

    2015-01-01

    We present new radial velocity measurements for 82 stars, members of the Galactic globular cluster (GC) NGC 6388, obtained from ESO-VLT K-band Multi Object Spectrograph (KMOS) spectra acquired during the instrument Science Verification. The accuracy of the wavelength calibration is discussed and a number of tests of the KMOS response are presented. The cluster systemic velocity obtained (81.3 ± 1.5 km s –1 ) is in very good agreement with previous determinations. While a hint of ordered rotation is found between 9'' and 20'' from the cluster center, where the distribution of radial velocities is clearly bimodal, more data are needed before drawing any firm conclusions. The acquired sample of radial velocities has also been used to determine the cluster velocity dispersion (VD) profile between ∼9'' and 70'', supplementing previous measurements at r < 2'' and r > 60'' obtained with ESO-SINFONI and ESO-FLAMES spectroscopy, respectively. The new portion of the VD profile nicely matches the previous ones, better defining the knee of the distribution. The present work clearly shows the effectiveness of a deployable integral field unit in measuring the radial velocities of individual stars for determining the VD profile of Galactic GCs. It represents the pilot project for an ongoing large program with KMOS and FLAMES at the ESO-VLT, aimed at determining the next generation of VD and rotation profiles for a representative sample of GCs

  16. Concurrent formation of supermassive stars and globular clusters: implications for early self-enrichment

    Science.gov (United States)

    Gieles, Mark; Charbonnel, Corinne; Krause, Martin G. H.; Hénault-Brunet, Vincent; Agertz, Oscar; Lamers, Henny J. G. L. M.; Bastian, Nathan; Gualandris, Alessia; Zocchi, Alice; Petts, James A.

    2018-04-01

    We present a model for the concurrent formation of globular clusters (GCs) and supermassive stars (SMSs, ≳ 103 M⊙) to address the origin of the HeCNONaMgAl abundance anomalies in GCs. GCs form in converging gas flows and accumulate low-angular momentum gas, which accretes onto protostars. This leads to an adiabatic contraction of the cluster and an increase of the stellar collision rate. A SMS can form via runaway collisions if the cluster reaches sufficiently high density before two-body relaxation halts the contraction. This condition is met if the number of stars ≳ 106 and the gas accretion rate ≳ 105 M⊙/Myr, reminiscent of GC formation in high gas-density environments, such as - but not restricted to - the early Universe. The strong SMS wind mixes with the inflowing pristine gas, such that the protostars accrete diluted hot-hydrogen burning yields of the SMS. Because of continuous rejuvenation, the amount of processed material liberated by the SMS can be an order of magnitude higher than its maximum mass. This `conveyor-belt' production of hot-hydrogen burning products provides a solution to the mass budget problem that plagues other scenarios. Additionally, the liberated material is mildly enriched in helium and relatively rich in other hot-hydrogen burning products, in agreement with abundances of GCs today. Finally, we find a super-linear scaling between the amount of processed material and cluster mass, providing an explanation for the observed increase of the fraction of processed material with GC mass. We discuss open questions of this new GC enrichment scenario and propose observational tests.

  17. CHEMICAL ABUNDANCES IN NGC 5053: A VERY METAL-POOR AND DYNAMICALLY COMPLEX GLOBULAR CLUSTER

    Energy Technology Data Exchange (ETDEWEB)

    Boberg, Owen M.; Friel, Eileen D.; Vesperini, Enrico [Astronomy Department, Indiana University, Bloomington, IN 47405 (United States)

    2015-05-10

    NGC 5053 provides a rich environment to test our understanding of the complex evolution of globular clusters (GCs). Recent studies have found that this cluster has interesting morphological features beyond the typical spherical distribution of GCs, suggesting that external tidal effects have played an important role in its evolution and current properties. Additionally, simulations have shown that NGC 5053 could be a likely candidate to belong to the Sagittarius dwarf galaxy (Sgr dSph) stream. Using the Wisconsin–Indiana–Yale–NOAO–Hydra multi-object spectrograph, we have collected high quality (signal-to-noise ratio ∼ 75–90), medium-resolution spectra for red giant branch stars in NGC 5053. Using these spectra we have measured the Fe, Ca, Ti, Ni, Ba, Na, and O abundances in the cluster. We measure an average cluster [Fe/H] abundance of −2.45 with a standard deviation of 0.04 dex, making NGC 5053 one of the most metal-poor GCs in the Milky Way (MW). The [Ca/Fe], [Ti/Fe], and [Ba/Fe] we measure are consistent with the abundances of MW halo stars at a similar metallicity, with alpha-enhanced ratios and slightly depleted [Ba/Fe]. The Na and O abundances show the Na–O anti-correlation found in most GCs. From our abundance analysis it appears that NGC 5053 is at least chemically similar to other GCs found in the MW. This does not, however, rule out NGC 5053 being associated with the Sgr dSph stream.

  18. Chemical Abundances in NGC 5053: A Very Metal-poor and Dynamically Complex Globular Cluster

    Science.gov (United States)

    Boberg, Owen M.; Friel, Eileen D.; Vesperini, Enrico

    2015-05-01

    NGC 5053 provides a rich environment to test our understanding of the complex evolution of globular clusters (GCs). Recent studies have found that this cluster has interesting morphological features beyond the typical spherical distribution of GCs, suggesting that external tidal effects have played an important role in its evolution and current properties. Additionally, simulations have shown that NGC 5053 could be a likely candidate to belong to the Sagittarius dwarf galaxy (Sgr dSph) stream. Using the Wisconsin-Indiana-Yale-NOAO-Hydra multi-object spectrograph, we have collected high quality (signal-to-noise ratio ˜ 75-90), medium-resolution spectra for red giant branch stars in NGC 5053. Using these spectra we have measured the Fe, Ca, Ti, Ni, Ba, Na, and O abundances in the cluster. We measure an average cluster [Fe/H] abundance of -2.45 with a standard deviation of 0.04 dex, making NGC 5053 one of the most metal-poor GCs in the Milky Way (MW). The [Ca/Fe], [Ti/Fe], and [Ba/Fe] we measure are consistent with the abundances of MW halo stars at a similar metallicity, with alpha-enhanced ratios and slightly depleted [Ba/Fe]. The Na and O abundances show the Na-O anti-correlation found in most GCs. From our abundance analysis it appears that NGC 5053 is at least chemically similar to other GCs found in the MW. This does not, however, rule out NGC 5053 being associated with the Sgr dSph stream.

  19. Galactic Pal-eontology: abundance analysis of the disrupting globular cluster Palomar 5

    Science.gov (United States)

    Koch, Andreas; Côté, Patrick

    2017-05-01

    We present a chemical abundance analysis of the tidally disrupted globular cluster (GC) Palomar 5. By co-adding high-resolution spectra of 15 member stars from the cluster's main body, taken at low signal-to-noise with the Keck/HIRES spectrograph, we were able to measure integrated abundance ratios of 24 species of 20 elements including all major nucleosynthetic channels (namely the light element Na; α-elements Mg, Si, Ca, Ti; Fe-peak and heavy elements Sc, V, Cr, Mn, Co, Ni, Cu, Zn; and the neutron-capture elements Y, Zr, Ba, La, Nd, Sm, Eu). The mean metallicity of -1.56 ± 0.02 ± 0.06 dex (statistical and systematic errors) agrees well with the values from individual, low-resolution measurements of individual stars, but it is lower than previous high-resolution results of a small number of stars in the literature. Comparison with Galactic halo stars and other disrupted and unperturbed GCs renders Pal 5 a typical representative of the Milky Way halo population, as has been noted before, emphasizing that the early chemical evolution of such clusters is decoupled from their later dynamical history. We also performed a test as to the detectability of light element variations in this co-added abundance analysis technique and found that this approach is not sensitive even in the presence of a broad range in sodium of 0.6 dex, a value typically found in the old halo GCs. Thus, while methods of determining the global abundance patterns of such objects are well suited to study their overall enrichment histories, chemical distinctions of their multiple stellar populations is still best obtained from measurements of individual stars. Full Table 3 is is only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/601/A41

  20. MASSIVE+: The Growth Histories of MASSIVE Survey Galaxies from their Globular Cluster Colors

    Science.gov (United States)

    Blakeslee, John

    2017-08-01

    The MASSIVE survey is targeting the 100 most massive galaxies within 108 Mpc that are visible in the northern sky. These most massive galaxies in the present-day universe reside in a surprisingly wide variety of environments, from rich clusters to fossil groups to near isolation. We propose to use WFC3/UVIS and ACS to carry out a deep imaging study of the globular cluster populations around a selected subset of the MASSIVE targets. Though much is known about GC systems of bright galaxies in rich clusters, we know surprisingly little about the effects of environment on these systems. The MASSIVE sample provides a golden opportunity to learn about the systematics of GC systems and what they can tell us about environmental drivers on the evolution of the highest mass galaxies. The most pressing questions to be addressed include: (1) Do isolated giants have the same constant mass fraction of GCs to total halo mass as BCGs of similar luminosity? (2) Do their GC systems show the same color (metallicity) distribution, which is an outcome of the mass spectrum of gas-rich halos during hierarchical growth? (3) Do the GCs in isolated high-mass galaxies follow the same radial distribution versus metallicity as in rich environments (a test of the relative importance of growth by accretion)? (4) Do the GCs of galaxies in sparse environments follow the same mass function? Our proposed second-band imaging will enable us to secure answers to these questions and add enormously to the legacy value of existing HST imaging of the highest mass galaxies in the universe.

  1. A Science Portal and Archive for Extragalactic Globular Cluster Systems Data

    Science.gov (United States)

    Young, Michael; Rhode, Katherine L.; Gopu, Arvind

    2015-01-01

    For several years we have been carrying out a wide-field imaging survey of the globular cluster populations of a sample of giant spiral, S0, and elliptical galaxies with distances of ~10-30 Mpc. We use mosaic CCD cameras on the WIYN 3.5-m and Kitt Peak 4-m telescopes to acquire deep BVR imaging of each galaxy and then analyze the data to derive global properties of the globular cluster system. In addition to measuring the total numbers, specific frequencies, spatial distributions, and color distributions for the globular cluster populations, we have produced deep, high-quality images and lists of tens to thousands of globular cluster candidates for the ~40 galaxies included in the survey.With the survey nearing completion, we have been exploring how to efficiently disseminate not only the overall results, but also all of the relevant data products, to the astronomical community. Here we present our solution: a scientific portal and archive for extragalactic globular cluster systems data. With a modern and intuitive web interface built on the same framework as the WIYN One Degree Imager Portal, Pipeline, and Archive (ODI-PPA), our system will provide public access to the survey results and the final stacked mosaic images of the target galaxies. In addition, the astrometric and photometric data for thousands of identified globular cluster candidates, as well as for all point sources detected in each field, will be indexed and searchable. Where available, spectroscopic follow-up data will be paired with the candidates. Advanced imaging tools will enable users to overlay the cluster candidates and other sources on the mosaic images within the web interface, while metadata charting tools will allow users to rapidly and seamlessly plot the survey results for each galaxy and the data for hundreds of thousands of individual sources. Finally, we will appeal to other researchers with similar data products and work toward making our portal a central repository for data

  2. Identification of antigenic Sarcoptes scabiei proteins for use in a diagnostic test and of non-antigenic proteins that may be immunomodulatory.

    OpenAIRE

    Marjorie S Morgan; S Dean Rider; Larry G Arlian

    2017-01-01

    Background Scabies, caused by the mite, Sarcoptes scabiei, infects millions of humans, and many wild and domestic mammals. Scabies mites burrow in the lower stratum corneum of the epidermis of the skin and are the source of substances that are antigenic or modulate aspects of the protective response of the host. Ordinary scabies is a difficult disease to diagnose. Objective The goal of this project was to identify S. scabiei proteins that may be candidate antigens for use in a diagnostic test...

  3. THE HELIUM CONTENT OF GLOBULAR CLUSTERS: NGC 6121 (M4)

    International Nuclear Information System (INIS)

    Villanova, S.; Geisler, D.; Piotto, G.; Gratton, R. G.

    2012-01-01

    In the context of the multiple stellar population scenario in globular clusters, helium (He) has been proposed as a key element to interpret the observed multiple main sequences, subgiant branches, and red giant branches, as well as the complex horizontal branch (HB) morphology. In particular, second-generation stars belonging to the bluer part of the HB are thought to be more He-rich (ΔY = 0.03 or more) but also more Na-rich/O-poor than those located in the redder part that should have Y equal to the cosmological value. Up to now this hypothesis was only partially confirmed in NGC 6752, where stars of the redder zero-age HB showed an He content of Y = 0.25 ± 0.01, fully compatible with the primordial He content of the universe, and were all Na-poor/O-rich. Here we study hot blue horizontal branch (BHB) stars in the GC NGC 6121 (M4) to measure their He plus O/Na content. Our goal is to complete the partial results obtained for NGC 6752, focusing our attention on targets located on the bluer part of the HB of M4. We observed six BHB stars using the VLT2/UVES spectroscopic facility. Spectra of signal-to-noise ratio ∼ 150 were obtained and the very weak He line at 5875 Å measured for all our targets. We compared this feature with synthetic spectra to obtain He abundances. In addition O, Na, and Fe abundances were estimated. Stars turned out to be all Na-rich and O-poor and to have a homogeneous He content with a mean value of Y = 0.29 ± 0.01(random) ± 0.01(systematic), which is enhanced by ΔY ∼ 0.04 with respect to the most recent measurements of the primordial He content of the universe (Y ∼ 0.24/0.25). The high He content of blue HB stars in M4 is also confirmed by the fact that they are brighter than red HB stars (RHB). Theoretical models suggest the BHB stars are He-enhanced by Δ(Y) = 0.02/0.03 with respect to the RHB stars. The whole sample of stars has a metallicity of [Fe/H] = –1.06 ± 0.02 (internal error), in agreement with other studies

  4. The Helium Content of Globular Clusters: NGC 6121 (M4)

    Science.gov (United States)

    Villanova, S.; Geisler, D.; Piotto, G.; Gratton, R. G.

    2012-03-01

    In the context of the multiple stellar population scenario in globular clusters, helium (He) has been proposed as a key element to interpret the observed multiple main sequences, subgiant branches, and red giant branches, as well as the complex horizontal branch (HB) morphology. In particular, second-generation stars belonging to the bluer part of the HB are thought to be more He-rich (ΔY = 0.03 or more) but also more Na-rich/O-poor than those located in the redder part that should have Y equal to the cosmological value. Up to now this hypothesis was only partially confirmed in NGC 6752, where stars of the redder zero-age HB showed an He content of Y = 0.25 ± 0.01, fully compatible with the primordial He content of the universe, and were all Na-poor/O-rich. Here we study hot blue horizontal branch (BHB) stars in the GC NGC 6121 (M4) to measure their He plus O/Na content. Our goal is to complete the partial results obtained for NGC 6752, focusing our attention on targets located on the bluer part of the HB of M4. We observed six BHB stars using the VLT2/UVES spectroscopic facility. Spectra of signal-to-noise ratio ~ 150 were obtained and the very weak He line at 5875 Å measured for all our targets. We compared this feature with synthetic spectra to obtain He abundances. In addition O, Na, and Fe abundances were estimated. Stars turned out to be all Na-rich and O-poor and to have a homogeneous He content with a mean value of Y = 0.29 ± 0.01(random) ± 0.01(systematic), which is enhanced by ΔY ~ 0.04 with respect to the most recent measurements of the primordial He content of the universe (Y ~ 0.24/0.25). The high He content of blue HB stars in M4 is also confirmed by the fact that they are brighter than red HB stars (RHB). Theoretical models suggest the BHB stars are He-enhanced by Δ(Y) = 0.02/0.03 with respect to the RHB stars. The whole sample of stars has a metallicity of [Fe/H] = -1.06 ± 0.02 (internal error), in agreement with other studies available in

  5. TRDistiller: a rapid filter for enrichment of sequence datasets with proteins containing tandem repeats.

    Science.gov (United States)

    Richard, François D; Kajava, Andrey V

    2014-06-01

    The dramatic growth of sequencing data evokes an urgent need to improve bioinformatics tools for large-scale proteome analysis. Over the last two decades, the foremost efforts of computer scientists were devoted to proteins with aperiodic sequences having globular 3D structures. However, a large portion of proteins contain periodic sequences representing arrays of repeats that are directly adjacent to each other (so called tandem repeats or TRs). These proteins frequently fold into elongated fibrous structures carrying different fundamental functions. Algorithms specific to the analysis of these regions are urgently required since the conventional approaches developed for globular domains have had limited success when applied to the TR regions. The protein TRs are frequently not perfect, containing a number of mutations, and some of them cannot be easily identified. To detect such "hidden" repeats several algorithms have been developed. However, the most sensitive among them are time-consuming and, therefore, inappropriate for large scale proteome analysis. To speed up the TR detection we developed a rapid filter that is based on the comparison of composition and order of short strings in the adjacent sequence motifs. Tests show that our filter discards up to 22.5% of proteins which are known to be without TRs while keeping almost all (99.2%) TR-containing sequences. Thus, we are able to decrease the size of the initial sequence dataset enriching it with TR-containing proteins which allows a faster subsequent TR detection by other methods. The program is available upon request. Copyright © 2014 Elsevier Inc. All rights reserved.

  6. Total protein concentration and diagnostic test results for gray wolf (Canis lupus) serum using Nobuto filter paper strips

    Science.gov (United States)

    Jara, Rocio F.; Sepúlveda, Carolina; Ip, Hon S.; Samuel, Michael D.

    2015-01-01

    Nobuto filter paper strips are widely used for storing blood-serum samples, but the recovery of proteins from these strips following rehydration is unknown. Poor recovery of proteins could reduce the concentration of antibodies and antigens and reduce the sensitivity of diagnostic assays. We compared the protein concentration, and its association with test sensitivity, of eluted Nobuto strip samples with paired sera. We collected and froze serum from five gray wolves (Canis lupus) for 8 mo. When thawed, we used a spectrophotometer (absorbance 280 nm) to determine the serum protein concentration for paired sera and Nobuto eluates for each animal in 2-fold serial dilutions. Total protein concentration was similar for both sample storage methods (Nobuto eluates and control sera), except for the undiluted samples in which Nobuto eluates had higher total protein concentrations. Both sample storage methods appear to produce similar results using the SNAP® 4Dx® Test to detect antibodies against pathogens causing Lyme disease, anaplasmosis, and ehrlichiosis as well as antigen for canine heartworm disease.

  7. Tidal stripping stellar substructures around four metal-poor globular clusters in the galactic bulge

    International Nuclear Information System (INIS)

    Chun, Sang-Hyun; Kang, Minhee; Jung, DooSeok; Sohn, Young-Jong

    2015-01-01

    We investigate the spatial density configuration of stars around four metal-poor globular clusters (NGC 6266, NGC 6626, NGC 6642, and NGC 6723) in the Galactic bulge region using wide-field deep J, H, and K imaging data obtained with the Wide Field Camera near-infrared array on the United Kingdom Infrared Telescope. A statistical weighted filtering algorithm for the stars on the color–magnitude diagram is applied in order to sort cluster member candidates from the field star contamination. In two-dimensional isodensity contour maps of the clusters, we find that all four of the globular clusters exhibit strong evidence of tidally stripped stellar features beyond the tidal radius in the form of tidal tails or small density lobes/chunks. The orientations of the extended stellar substructures are likely to be associated with the effect of dynamic interaction with the Galaxy and the cluster's space motion. The observed radial density profiles of the four globular clusters also describe the extended substructures; they depart from theoretical King and Wilson models and have an overdensity feature with a break in the slope of the profile at the outer region of clusters. The observed results could imply that four globular clusters in the Galactic bulge region have experienced strong environmental effects such as tidal forces or bulge/disk shocks of the Galaxy during the dynamical evolution of globular clusters. These observational results provide further details which add to our understanding of the evolution of clusters in the Galactic bulge region as well as the formation of the Galaxy.

  8. The evoluation of the galactic globular clusters; I Metal abundance calibrations

    International Nuclear Information System (INIS)

    Lee, S.W.; Park, N.K.

    1984-01-01

    Five different calibrations of metal abundances of globular clusters are examined and these are compared with metallicity ranking parameters such as (Sp)sub(c), , Q39 and IR-indices. Except for the calibration *(Fe/H*)sub(H) by the high dispersion echelle analysis, the other calibration scales are correlated with the morphological parameters of red giant branch. In the *(Fe/H*)sub(Hsup(-))scale, the clusters later than approx.F8 have nearly a constant metal abundance, *(Fe/H*)sub(H)approx.-1.05, regardless of morphological characteristics of horizontal branch and red giant branch. By the two fundamental calibration scales of *(Fe/H*)sub(L) (derived by the low dispersion analysis), and *(Fe/H*)sub(delta S) (derived by the spectral analysis of RR Lyrae stars), the globular clusters are divided into the halo clusters with *(Fe/H*)<-1.0 and the disk clusters confined within the galactocentric distance rsub(G)=10 kpc and galactic plane distance absolute z=3 kpc. In this case the abundance gradient is given by d*(Fe/H*)/drsub(G)approx.-0.05kpcsup(-1) and d*(Fe/H*)/d absolute z approx. -0.08 kpcsup(-1) within rsub(G)=20 kpc and absolute z=10 kpc, respectively. According to these characteristics of the spatial distribution of globular clusters, the chemical evolution of the galactic globular clusters can be accounted for by the two-zone (disk-halo) slow collapse model when the *(Fe/H*)sub(Lsup(-)) or *(Fe/H*)sub(DELTA Ssup(-))scale is applied. In the case of *(Fe/H*)sub(Hsup(-))scale, the one-zone fast collapse model is preferred for the evolution of globular clusters. (Author)

  9. Identification and monitoring of host cell proteins by mass spectrometry combined with high performance immunochemistry testing.

    Directory of Open Access Journals (Sweden)

    Katrin Bomans

    Full Text Available Biotherapeutics are often produced in non-human host cells like Escherichia coli, yeast, and various mammalian cell lines. A major focus of any therapeutic protein purification process is to reduce host cell proteins to an acceptable low level. In this study, various E. coli host cell proteins were identified at different purifications steps by HPLC fractionation, SDS-PAGE analysis, and tryptic peptide mapping combined with online liquid chromatography mass spectrometry (LC-MS. However, no host cell proteins could be verified by direct LC-MS analysis of final drug substance material. In contrast, the application of affinity enrichment chromatography prior to comprehensive LC-MS was adequate to identify several low abundant host cell proteins at the final drug substance level. Bacterial alkaline phosphatase (BAP was identified as being the most abundant host cell protein at several purification steps. Thus, we firstly established two different assays for enzymatic and immunological BAP monitoring using the cobas® technology. By using this strategy we were able to demonstrate an almost complete removal of BAP enzymatic activity by the established therapeutic protein purification process. In summary, the impact of fermentation, purification, and formulation conditions on host cell protein removal and biological activity can be conducted by monitoring process-specific host cell proteins in a GMP-compatible and high-throughput (> 1000 samples/day manner.

  10. Neutron-Capture Nucleosynthesis and the Chemical Evolution of Globular Clusters

    Science.gov (United States)

    Shingles, Luke J.

    2015-09-01

    Elements heavier than iron are almost entirely produced in stars through neutron captures and radioactive decays. Of these heavy elements, roughly half are produced by the slow neutron-capture process (s-process), which takes place under extended exposure to low neutron densities. Most of the s-process production occurs in stars with initial masses between roughly 0.8 and 8 solar masses (Msun), which evolve through the Asymptotic Giant Branch (AGB) phase. This thesis explores several topics related to AGB stars and the s-process, with a focus on comparing theoretical models to observations in the literature on planetary nebulae, post-AGB stars, and globular cluster stars. A recurring theme is the uncertainty of carbon-13-pocket formation, which is crucial for building accurate models of s-process nucleosynthesis. We first investigated whether neutron-capture reactions in AGB stars are the cause of the low sulphur abundances in planetary nebulae and post-AGB stars relative to the interstellar medium. Accounting for uncertainties in the size of the partial mixing zone that forms carbon-13 pockets and the rates of neutron-capture and neutron-producing reactions, our models failed to reproduce the observed levels of sulphur destruction. From this, we concluded that AGB nucleosynthesis is not the cause of the sulphur anomaly. We also discovered a new method to constrain the extent of the partial mixing zone using neon abundances in planetary nebulae. We next aimed to discover the stellar sites of the s-process enrichment in globular clusters that have inter- and intra-cluster variation, with the examples of M4 (relative to M5) and M22, respectively. Using a new chemical evolution code developed by the candidate, we tested models with stellar yields from rotating massive stars and AGB stars. We compared our model predictions for the production of s-process elements with abundances from s-poor and s-rich populations. We found that rotating massive stars alone do not

  11. Short-term X-ray variability of the globular cluster source 4U 1820 - 30 (NGC 6624)

    Science.gov (United States)

    Stella, L.; Kahn, S. M.; Grindlay, J. E.

    1984-01-01

    Analytical techniques for improved identification of the temporal and spectral variability properties of globular cluster and galactic bulge X-ray sources are described in terms of their application to a large set of observations of the source 4U 1820 - 30 in the globular cluster NGC 6624. The autocorrelation function, cross-correlations, time skewness function, erratic periodicities, and pulse trains are examined. The results are discussed in terms of current models with particular emphasis on recent accretion disk models. It is concluded that the analyzed observations provide the first evidence for shot-noise variability in a globular cluster X-ray source.

  12. Predicting protein folding rate change upon point mutation using residue-level coevolutionary information.

    Science.gov (United States)

    Mallik, Saurav; Das, Smita; Kundu, Sudip

    2016-01-01

    Change in folding kinetics of globular proteins upon point mutation is crucial to a wide spectrum of biological research, such as protein misfolding, toxicity, and aggregations. Here we seek to address whether residue-level coevolutionary information of globular proteins can be informative to folding rate changes upon point mutations. Generating residue-level coevolutionary networks of globular proteins, we analyze three parameters: relative coevolution order (rCEO), network density (ND), and characteristic path length (CPL). A point mutation is considered to be equivalent to a node deletion of this network and respective percentage changes in rCEO, ND, CPL are found linearly correlated (0.84, 0.73, and -0.61, respectively) with experimental folding rate changes. The three parameters predict the folding rate change upon a point mutation with 0.031, 0.045, and 0.059 standard errors, respectively. © 2015 Wiley Periodicals, Inc.

  13. Gastric clearance of alpha-1-antitrypsin under cimetidine perfusion. New test to detect protein-losing gastropathy

    International Nuclear Information System (INIS)

    Florent, C.; Vidon, N.; Flourie, B.; Carmantrand, A.; Zerbani, A.; Maurel, M.; Bernier, J.J.

    1986-01-01

    Gastric losses of plasma are usually measured with radiolabeled macromolecules. This method is expensive and cumbersome. Direct measurement of exudated plasma proteins are ineffective since proteins are denaturated by acidic gastric juice and pepsin. It was recently shown that albumin measurement after immediate neutralization allowed detection of gastric protein losses, but this method is quite complex and time consuming. We studied alpha 1-antitrypsin and 51Cr-labeled protein clearance in gastric juice during normal saline and cimetidine (1.5 mg/kg/hr) infusion in six healthy volunteers and six patients with exudative gastropathy. alpha 1-Antitrypsin was measurable in all samples during cimetidine infusion: alpha 1-AT and 51Cr losses were significantly correlated (P less than 0.001). The upper limit of gastric alpha 1-AT clearance in controls was 0.86 ml/hr (mean + 2 SD). Using this value, there was no overlapping between patients and controls. The upper limit of 51Cr test was 1.87 ml/hr (mean + 2 SD) in controls but gastric clearance of 51Cr was below this value in one patient. This suggests that the measurement of alpha 1-AT gastric clearance during cimetidine perfusion is a good test to detect an exudative gastropathy. This test is inexpensive and lasts only 3 hr

  14. Protein misfolding and disease: from the test tube to the organism.

    Science.gov (United States)

    Luheshi, Leila M; Crowther, Damian C; Dobson, Christopher M

    2008-02-01

    Protein misfolding is the underlying cause of many highly debilitating disorders ranging from Alzheimer's Disease to Cystic Fibrosis. Great strides have been made recently in understanding what causes proteins to misfold, primarily through the use of biophysical and computational techniques that enable systematic and quantitative analysis of the effects of a range of different perturbations in proteins. Correlation of the results of such analyses with observations made in animal models of disease has however been limited by their seemingly irreconcilable differences in methodology and scope. Several recent studies have however begun to overcome this limitation by combining the two approaches. This strategy has made it possible to investigate many of the consequences of protein misfolding in vivo, ranging from disease pathogenesis to epigenetic regulation, in the context of the fundamental physico-chemical principles derived from extensive and highly detailed studies undertaken in vitro.

  15. TIME-SERIES PHOTOMETRY OF GLOBULAR CLUSTERS: M62 (NGC 6266), THE MOST RR LYRAE-RICH GLOBULAR CLUSTER IN THE GALAXY?

    International Nuclear Information System (INIS)

    Contreras, R.; Catelan, M.; Smith, H. A.; Kuehn, C. A.; Pritzl, B. J.; Borissova, J.

    2010-01-01

    We present new time-series CCD photometry, in the B and V bands, for the moderately metal-rich ([Fe/H] ≅ -1.3) Galactic globular cluster M62 (NGC 6266). The present data set is the largest obtained so far for this cluster and consists of 168 images per filter, obtained with the Warsaw 1.3 m telescope at the Las Campanas Observatory and the 1.3 m telescope of the Cerro Tololo Inter-American Observatory, in two separate runs over the time span of 3 months. The procedure adopted to detect the variable stars was the optimal image subtraction method (ISIS v2.2), as implemented by Alard. The photometry was performed using both ISIS and Stetson's DAOPHOT/ALLFRAME package. We have identified 245 variable stars in the cluster fields that have been analyzed so far, of which 179 are new discoveries. Of these variables, 133 are fundamental mode RR Lyrae stars (RRab), 76 are first overtone (RRc) pulsators, 4 are type II Cepheids, 25 are long-period variables (LPVs), 1 is an eclipsing binary, and 6 are not yet well classified. Such a large number of RR Lyrae stars places M62 among the top two most RR Lyrae-rich (in the sense of total number of RR Lyrae stars present) globular clusters known in the Galaxy, second only to M3 (NGC 5272) with a total of 230 known RR Lyrae stars. Since this study covers most but not all of the cluster area, it is not unlikely that M62 is in fact the most RR Lyrae-rich globular cluster in the Galaxy. In like vein, thanks to the time coverage of our data sets, we were also able to detect the largest sample of LPVs known so far in a Galactic globular cluster. We analyze a variety of Oosterhoff type indicators for the cluster, including mean periods, period distribution, Bailey diagrams, and Fourier decomposition parameters (as well as the physical parameters derived therefrom). All of these indicators clearly show that M62 is an Oosterhoff type I system. This is in good agreement with the moderately high metallicity of the cluster, in spite of its

  16. Use of recombinant CFP-10 protein for a skin test specific for ...

    African Journals Online (AJOL)

    Jane

    2010-10-18

    Oct 18, 2010 ... molecular masses of the protein standards ranged from 6 to 97.4. kDa (Sigma, St Louis, MO, USA). Gels were stained with. Coomassie blue. The molecular mass and purity of rCFP-10 protein were analyzed and calculated with Biologic software TotalLab v1.11. The molecular mass of purified rCFP-10 ...

  17. Preclinical Testing of a Translocator Protein Ligand for the Treatment of Amyotrophic Lateral Sclerosis

    Science.gov (United States)

    2017-03-01

    investigated the neuroprotective effect and therapeutic value of a drug : the translocator protein (TSPO) ligand PK11195 (PK), which we found to be...Translocator protein (TSPO) ligand Neuroprotective drugs In vitro models of neurodegeneration Axon preservation Neuromuscular junctions 5 3...pharmacokinetic data of the JNK study were showing that daily gavage (30 mg/kg/day) allowed an efficient delivery of the drugs in the brain and the spinal

  18. Testing the utility of fluorescent proteins in Mimulus lewisii by an Agrobacterium-mediated transient assay.

    Science.gov (United States)

    Ding, Baoqing; Yuan, Yao-Wu

    2016-04-01

    The Agrobacterium -mediated transient expression assay by leaf infiltration in Mimulus lewisii is robust. Fluorescent proteins EGFP, EYFP and DsRed give bright fluorescence signals in the infiltrated tissue. Mimulus lewisii is an emerging developmental genetic model system. Recently developed genomic and genetic resources and a stable transformation protocol have greatly facilitated the identification and functional characterization of genes controlling the development of ecologically important floral traits using this species. To further expedite gene and protein function analyses in M. lewisii, we adopted and simplified the Agrobacterium-mediated transient gene expression method routinely used in tobacco plants. With the validated transient assay, we examined the performance of fluorescent proteins EGFP, EYFP and DsRed in M. lewisii. All three proteins gave bright fluorescence signals when transiently expressed in agroinfiltrated leaves. Furthermore, we demonstrated the utility of fluorescent proteins in M. lewisii by showing the nuclear localization of Reduced Carotenoid Pigmentation 1 (RCP1), a recently discovered R2R3-MYB transcription factor that regulates carotenoid pigmentation during flower development. Both the transient assay and the fluorescent proteins are valuable additions to the M. lewisii toolbox, making this emerging genetic and developmental model system even more powerful.

  19. A period-luminosity relation for Mira variables in globular clusters and its impact on the distance scale

    International Nuclear Information System (INIS)

    Menzies, J.W.; Whitelock, P.A.

    1985-01-01

    JHKL photometry is presented for 31 red variables in 15 galactic globular clusters. The photometry of the Mira variables is used to find absolute bolometric magnitudes and an Msub(bol)-log P relation which differs from the one found for LMC Miras. This can be understood only if there is some systematic error in the globular cluster and/or LMC distance scales or if there is some fundamental difference between the cluster Miras and those in the LMC. (author)

  20. A mode of error: Immunoglobulin binding protein (a subset of anti-citrullinated proteins can cause false positive tuberculosis test results in rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    Maria Greenwald

    2017-12-01

    Full Text Available Citrullinated Immunoglobulin Binding Protein (BiP is a newly described autoimmune target in rheumatoid arthritis (RA, one of many cyclic citrullinated peptides(CCP or ACPA. BiP is over-expressed in RA patients causing T cell expansion and increased interferon levels during incubation for the QuantiFERON-Gold tuberculosis test (QFT-G TB. The QFT-G TB has never been validated where interferon is increased by underlying disease, as for example RA.Of ACPA-positive RA patients (n = 126, we found a 13% false-positive TB test rate by QFT-G TB. Despite subsequent biologic therapy for 3 years of all 126 RA patients, none showed evidence of TB without INH. Most of the false-positive RA patients after treatment with biologic therapy reverted to a negative QFT-G test. False TB tests correlated with ACPA level (p < 0.02.Three healthy women without arthritis or TB exposure had negative QFT-G TB. In vitro, all three tested positive every time for TB correlating to the dose of BiP or anti-BiP added, at 2 ug/ml, 5 ug/ml, 10 ug/ml, and 20 ug/ml.BiP naturally found in the majority of ACPA-positive RA patients can result in a false positive QFT-G TB. Subsequent undertreatment of RA, if biologic therapy is withheld, and overtreatment of presumed latent TB may harm patients. Keywords: Tuberculosis, IGRA, Rheumatoid arthritis, Interferon, Anti-citrullinated peptide antibody (ACPA, Immunoglobulin binding protein (BiP

  1. Expected packing density allows prediction of both amyloidogenic and disordered regions in protein chains

    Energy Technology Data Exchange (ETDEWEB)

    Galzitskaya, Oxana V; Garbuzynskiy, Sergiy O; Lobanov, Michail Yu [Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region (Russian Federation)

    2007-07-18

    The determination of factors that influence conformational changes in proteins is very important for the identification of potentially amyloidogenic and disordered regions in polypeptide chains. In our work we introduce a new parameter, mean packing density, to detect both amyloidogenic and disordered regions in a protein sequence. It has been shown that regions with strong expected packing density are responsible for amyloid formation. Our predictions are consistent with known disease-related amyloidogenic regions for 9 of 12 amyloid-forming proteins and peptides in which the positions of amyloidogenic regions have been revealed experimentally. Our findings support the concept that the mechanism of formation of amyloid fibrils is similar for different peptides and proteins. Moreover, we have demonstrated that regions with weak expected packing density are responsible for the appearance of disordered regions. Our method has been tested on datasets of globular proteins and long disordered protein segments, and it shows improved performance over other widely used methods. Thus, we demonstrate that the expected packing density is a useful value for predicting both disordered and amyloidogenic regions of a protein based on sequence alone. Our results are important for understanding the structural characteristics of protein folding and misfolding.

  2. Effect of soy protein isolate in the diet on retention by the rat of iron from radiolabeled test meals

    International Nuclear Information System (INIS)

    Thompson, D.B.; Erdman, J.W. Jr.

    1984-01-01

    The influence of soy protein isolate (SPI) in the diet on whole-body retention of extrinsically radiolabeled iron from test meals containing or not containing SPI was evaluated in marginally iron-deficient weanling rats. In experiment 1 SPI was compared with casein in a 2 X 2 factorial design: diets and test meals were either SPI-based or casein-based. Diets were fed for 13 days prior to the test meal and for 7 days subsequent to the test meal. Rats fed the SPI-based diet retained less iron from test meals than did rats fed the casein-based diet (66.1 vs. 74.8%, P less than 0.01). Experiment 2 showed that an SPI-based diet fed during the final 4 days of a 14-day pre-test meal period and subsequent to the test meal led to less iron retention compared to a casein-based diet. In addition to the observed diet effect, experiment 1 showed that iron retention was less from an SPI-based test meal than from a casein-based test meal, confirming previous reports of adverse effects of SPI on iron retention. The present experiments show that SPI can adversely affect from retention in two ways: by its presence in the diet before and after a test meal, and by its presence in a test meal

  3. Extraction methods and test techniques for detection of vegetable proteins in meat products. I. Qualitative detection of soya derivatives.

    Science.gov (United States)

    Hyslop, N S

    1976-06-01

    Extracts of 3 soya bean preparations, used commercially in certain countries to replace part of the meat in popular meat products, were made by treatment with (i) sodium dodecyl sulphate, (ii) Triton-X100 or (iii) n-Butanol. Similar extracts were made from beef and pork. All extracts were examined by electrophoretic and immunological techniques. Stained polyacrylamide gels revealed distinctive protein bands after electrophoresis. The migration rates of corresponding bands differed between beef and pork extracts. However, the migration rates of vegetable bands revealed certain similarities, but differed very greatly from those of animal origin. Characteristic fast-migrating S-bands were distinguishable only in extracts of vegetable protein. Immunodiffusion tests, using antisera produced in rabbits against each extract, revealed varying degrees of similarity between extracts of vegetable origin, but the antisera were specific for either vegetable or animal protein.

  4. New bound on neutrino dipole moments from globular-cluster stars

    Science.gov (United States)

    Raffelt, Georg G.

    1990-01-01

    Neutrino dipole moments mu(nu) would increase the core mass of red giants at the helium flash by delta(Mc) = 0.015 solar mass x mu(nu)/10 to the -12th muB (where muB is the Bohr magneton) because of enhanced neutrino losses. Existing measurements of the bolometric magnitudes of the brightest red giants in 26 globular clusters, number counts of horizontal-branch stars and red giants in 15 globular clusters, and statistical parallax determinations of field RR Lyr luminosities yield delta(Mc) = 0.009 + or - 0.012 solar mass, so that conservatively mu(nu) is less than 3 x 10 to the -12th muB.

  5. THE IMPACT OF CONTAMINATED RR LYRAE/GLOBULAR CLUSTER PHOTOMETRY ON THE DISTANCE SCALE

    Energy Technology Data Exchange (ETDEWEB)

    Majaess, D.; Turner, D.; Lane, D. [Department of Astronomy and Physics, Saint Mary' s University, Halifax, NS B3H 3C3 (Canada); Gieren, W., E-mail: dmajaess@ap.smu.ca [Departamento de Astronomia, Universidad de Concepcion, Casilla 160-C, CL Concepcion (Chile)

    2012-06-10

    RR Lyrae variables and the stellar constituents of globular clusters are employed to establish the cosmic distance scale and age of the universe. However, photometry for RR Lyrae variables in the globular clusters M3, M15, M54, M92, NGC 2419, and NGC 6441 exhibit a dependence on the clustercentric distance. For example, variables and stars positioned near the crowded high-surface brightness cores of the clusters may suffer from photometric contamination, which invariably affects a suite of inferred parameters (e.g., distance, color excess, absolute magnitude, etc.). The impetus for this study is to mitigate the propagation of systematic uncertainties by increasing awareness of the pernicious impact of contaminated and radial-dependent photometry.

  6. THE INFLUENCE OF POTASSIUM DICHROMATE Cr (VI ADMINISTRATION DURATION ON GLOBULAR RESISTANCE IN FEMALE RATS

    Directory of Open Access Journals (Sweden)

    LETIŢIA STANA

    2009-05-01

    Full Text Available The „in vivo” experiment has had as aim the study of different Cr(VI doses administration on globular resistance in female rats related to administration duration. Study was carried out on 56 female rats divided in 8 groups, 6 experimental and 2 control that received potassium dichromate in drinking water in doses of 25 ppm, 50 ppm and 75ppm Cr(VI, for 3 months, respectively, 6 months. Decrease of globular resistance (in terms of haemolysis degree in hypotonic solutions at increasing dose (up to 0.8% NaCl at 75 ppm dose in all experimental groups, in direct relation with the duration of administration was registered. Control groups were in physiological limits. The results of the present study revealed the affecting of erythrocyte membrane in function of administration duration and chromium intake level, because of oxidative lesions produced by it.

  7. Another non-segregated Blue Straggler population in a globular cluster: the case of NGC 2419.

    Science.gov (United States)

    Dalessandro, E.; Lanzoni, B.; Ferraro, F. R.; Vespe, F.; Bellazzini, M.; Rood, R. T.

    We have used a combination of ACS-HST high-resolution and wide-field SUBARU data in order to study the Blue Straggler Star (BSS) population over the entire extension of the remote Galactic globular cluster NGC 2419. The radial distribution of the selected BSS is the same as that of the other cluster stars. In this sense the BSS radial distribution is like that of omega Centauri and unlike that of all Galactic globular clusters studied to date, which have highly centrally segregated distributions and in most cases a pronounced upturn in the external regions. As in the case of omega Centauri, this evidence indicates that NGC 2419 is not yet relaxed even in the central regions. This observational fact is in agreement with estimated half-mass relaxation time, which is of the order of the cluster age.

  8. Globular cluster neutron stars and the determination of the dense matter equation of state

    Science.gov (United States)

    Guillot, Sebastien

    2016-09-01

    Combining measurements of the mass and radius of multiple neutron stars (NSs) represents the most promising way to determine the equation of state of dense NS matter. NSs in quiescent low-mass x-ray binaries (qLMXB) located in globular clusters have placed useful constraints on the equation of state. The statistical approaches combining measurements from multiple NSs can be further improved by the addition of more NS observations. We propose here to obtain a high signal to noise spectrum of the qLMXB in M30, the only low-absorption globular cluster qLMXBs that does not have deep X-ray observations, and which requires Chandra unmatched angular resolution. The 300 ks proposed observation will permit measurement of the NS radius with 12-15% uncertainties.

  9. Intrachromosomal exchange aberrations predicted on the basis of globular interphase chromosome model

    International Nuclear Information System (INIS)

    Andreev, S.G.; Eidelman, Yu.A.

    2002-01-01

    One of the key questions in understanding mechanisms of chromosome aberration production is how does interphase chromosome structure affect aberration formation. To explore this a modelling approach is presented which combines Monte Carlo simulation of both a particle track and interphase chromosome structure. The structural state of interphase chromosome influences a dose-effect relationship for intrachromosomal exchange aberrations (intrachanges). It is shown that intrachanges are induced frequently by both X rays and a particles if the chromosome is in the condensed globular but not in the decondensed coiled state. Truly simple intra-arm intrachanges induced by X rays are dose squared in coiled chromosomes, but exhibit linear dose dependence in globular chromosomes. Experimental data on interarm intrachanges obtained by dual arm chromosome painting are analysed by means of the technique presented. Results of analysis support the conclusion about the arms proximity of chromosome 1 in human lymphocytes. (author)

  10. Transcription and expression of Plasmodium falciparum histidine-rich proteins in different stages and strains: implications for rapid diagnostic tests.

    Directory of Open Access Journals (Sweden)

    Joanne Baker

    Full Text Available BACKGROUND: Although rapid diagnostic tests (RDTs for Plasmodium falciparum infection that target histidine rich protein 2 (PfHRP2 are generally sensitive, their performance has been reported to be variable. One possible explanation for variable test performance is differences in expression level of PfHRP in different parasite isolates. METHODS: Total RNA and protein were extracted from synchronised cultures of 7 P. falciparum lines over 5 time points of the life cycle, and from synchronised ring stages of 10 falciparum lines. Using quantitative real-time polymerase chain reaction, Western blot analysis and ELISA we investigated variations in the transcription and protein levels of pfhrp2, pfhrp3 and PfHRP respectively in the different parasite lines, over the parasite intraerythrocytic life cycle. RESULTS: Transcription of pfhrp2 and pfhrp3 in different parasite lines over the parasite life cycle was observed to vary relative to the control parasite K1. In some parasite lines very low transcription of these genes was observed. The peak transcription was observed in ring-stage parasites. Pfhrp2 transcription was observed to be consistently higher than pfhrp3 transcription within parasite lines. The intraerythrocytic lifecycle stage at which the peak level of protein was present varied across strains. Total protein levels were more constant relative to total mRNA transcription, however a maximum 24 fold difference in expression at ring-stage parasites relative to the K1 strain was observed. CONCLUSIONS: The levels of transcription of pfhrp2 and pfhrp3, and protein expression of PfHRP varied between different P. falciparum strains. This variation may impact on the detection sensitivity of PfHRP2-detecting RDTs.

  11. Changes in the protein profile of Habanero pepper (Capsicum ...

    African Journals Online (AJOL)

    2012-06-12

    Jun 12, 2012 ... (Capsicum chinense J.) somatic embryos during ... molecular weights to those reported for storage proteins in other .... were isolated by cutting with a razor blade. ... electrophoresis (SDS-PAGE; 5% stacking gel [pH 6.8], 15% running ... Developmental stages correspond to C) globular, D) heart-shaped, ...

  12. Structure and dynamics of interfacial water. Role of hydratation water in the globular proteins dynamics

    International Nuclear Information System (INIS)

    Zanotti, J.M.

    1997-01-01

    This memoir includes five chapters. In the first chapter, are given the elements of the neutrons scattering theory that is used in this study. the second chapter is devoted to a general presentation of the interaction between biological macro molecule and water. The third part is dedicated to the study of the structure and the dynamics of interfacial water in the neighbouring of model systems, the vycor and the amorphous carbon. The results presented in this part are compared with these one relative to water dynamics at the C-phycocyanin surface. This study makes the object of the fourth chapter. Then, in the fifth and last chapter are discussed the results relative to the role of hydratation on the parv-albumin dynamics for which have been combined the neutron quasi elastic incoherent scattering and the nuclear magnetic resonance of the carbon 13 solid in natural abundance

  13. Temperature-dependent dynamical transitions of different classes of amino acid residue in a globular protein.

    Science.gov (United States)

    Miao, Yinglong; Yi, Zheng; Glass, Dennis C; Hong, Liang; Tyagi, Madhusudan; Baudry, Jerome; Jain, Nitin; Smith, Jeremy C

    2012-12-05

    The temperature dependences of the nanosecond dynamics of different chemical classes of amino acid residue have been analyzed by combining elastic incoherent neutron scattering experiments with molecular dynamics simulations on cytochrome P450cam. At T = 100-160 K, anharmonic motion in hydrophobic and aromatic residues is activated, whereas hydrophilic residue motions are suppressed because of hydrogen-bonding interactions. In contrast, at T = 180-220 K, water-activated jumps of hydrophilic side chains, which are strongly coupled to the relaxation rates of the hydrogen bonds they form with hydration water, become apparent. Thus, with increasing temperature, first the hydrophobic core awakens, followed by the hydrophilic surface.

  14. First-Principles Predictions of Vibrational Raman Optical Activity of Globular Proteins

    Czech Academy of Sciences Publication Activity Database

    Kessler, Jiří; Kapitán, J.; Bouř, Petr

    2015-01-01

    Roč. 6, č. 16 (2015), s. 3314-3319 ISSN 1948-7185 R&D Projects: GA ČR GAP208/11/0105; GA ČR GA15-09072S Grant - others:GA MŠk(CZ) LM2010005; GA MŠk(CZ) ED3.2.00/08.0144 Institutional support: RVO:61388963 Keywords : molecular property tensors * activity spectra * spectroscopy Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 8.539, year: 2015 http://pubs.acs.org/doi/pdf/10.1021/acs.jpclett.5b01500

  15. The pulsation mode and period-luminosity relationship of cool variables in globular clusters

    International Nuclear Information System (INIS)

    Whitelock, P.A.

    1986-01-01

    The period-luminosity-temperature relationship for globular cluster red and yellow variables is examined. The results suggest that the higher temperature, more metal-deficient cluster variables pulsate in the fundamental mode, while the lower temperature more metal-rich variables pulsate in the first overtone. On the assumption that this is correct, a relationship between fundamental period and bolometric magnitude is derived for cluster variables with observed periods of between 1 and 300 days. (author)

  16. Globular hepatic amyloid is highly sensitive and specific for LECT2 amyloidosis.

    Science.gov (United States)

    Chandan, Vishal S; Shah, Sejal S; Lam-Himlin, Dora M; Petris, Giovanni De; Mereuta, Oana M; Dogan, Ahmet; Torbenson, Michael S; Wu, Tsung-Teh

    2015-04-01

    Globular hepatic amyloid (GHA) is rare, and its clinical significance remains unclear. Recently, leukocyte chemotactic factor-associated amyloidosis (ALECT2) has been reported to involve the liver, showing a globular pattern. We reviewed 70 consecutive cases of hepatic amyloidosis to determine the prevalence and morphology of hepatic amyloid subtypes, especially ALECT2 and its association with GHA. Each case was reviewed for amyloid subtype (immunohistochemistry and/or mass spectrometry), its pattern (linear or globular), and distribution (vascular, perisinusoidal, or stromal). In addition, 24 cases of confirmed hepatic ALECT2 on mass spectrometry from our consultation files were also reviewed. LECT2 immunostaining was performed in 49 cases. Of the 70 cases, immunoglobulin light chain (AL) type was most common with 41 cases (59%), followed by transthyretin (ATTR) 15 cases (22%), 3 cases each of fibrinogen A (AFib) (4%), serum amyloid A (AA) (4%), and ALECT2 (4%), 2 cases of apolipoproteins (AApoA1) (3%), and 3 cases (4%) were unclassified. Three of our 70 cases (4%), with ALECT2, and all 24 cases (100%) of mass spectrometry-confirmed hepatic ALECT2 showed only GHA deposits in the hepatic sinusoids and portal tracts. Three (4%) other cases of AL type showed a focal globular pattern admixed with prominent linear amyloid. None of the other amyloid subtypes showed GHA. LECT2 immunostain was positive in all 27 cases (100%) of ALECT2 and negative in the other 22 non-ALECT2 cases (100%) (14 AL, 5 ATTR, 1 AA, 1 AFib, 1 AApoA1). Pure GHA is uncommon (4%) but is highly specific for ALECT2, and LECT2 immunostain is helpful in confirming this amyloid type.

  17. Parâmetros astrofísicos de estrelas gigantes do aglomerado globular 47 Tucanae

    Science.gov (United States)

    Alves-Brito, A.; Barbuy, B.

    2003-08-01

    Os aglomerados globulares são considerados laboratórios astrofísicos para a verificação da teoria de evolução estelar, bem como a trajetória químio-dinâmica das galáxias hospedeiras. Em particular, 47 Tucanae (NGC 104) configura-se como um dos mais extensivamente estudados aglomerados globulares da Galáxia devido a relativa proximidade ao Sol (R¤ = 4.5 kpc) e alta latitute galáctica (b = -44°,89). Neste trabalho, apresentamos a velocidade radial heliocêntrica e os parâmetros atmosféricos (Teff, logg, [Fe/H]) de 5 estrelas gigantes do aglomerado globular 47 Tucanae. Os espectros foram obtidos pelo espectrógrafo UVES (Ultaviolet Visual Echelle Spectrograph) de alta resolução (R = 60000) e alta razão sinal-ruído (S/N > 200), acoplado ao telescópio de 8,2m Kueyen do VLT (Very Large Telescope). Nós encontramos = -22,43 +/- 3,97 km/s, [Fe/H] ~ -0.7, 1,2 estrelas cobrem um intervalo de magnitude 12,2 < V < 14,2. Os parâmetros atmosféricos são fundamentais para a construção de espectros sintéticos de outros aglomerados globulares ricos em metais. Trabalho financiado pela FAPESP e pelo CNPq.

  18. BVRI CCD photometry of the metal-poor globular cluster M68 (NGC 4590)

    International Nuclear Information System (INIS)

    Alcaino, G.; Liller, W.; Alvarado, F.; Wenderoth, E.

    1990-01-01

    BVRI photometry of the low metallicity globular cluster M68 (NGC 4590) was obtained with a CCD camera and the 2.2-m ESO telescope. The resulting BV color-magnitude diagrams are compared with the observations of McClure et al. (1987). The observations are also compared with theoretical isochrones, yielding a cluster age of 13 Gyr with a likely external uncertainty of 2 or 3 Gyr. 25 refs

  19. NGC 6273: Towards Defining A New Class of Galactic Globular Clusters?

    Science.gov (United States)

    Johnson, Christian I.; Rich, Robert Michael; Pilachowski, Catherine A.; Caldwell, Nelson; Mateo, Mario L.; Ira Bailey, John; Crane, Jeffrey D.

    2016-01-01

    A growing number of observations have found that several Galactic globular clusters exhibit abundance dispersions beyond the well-known light element (anti-)correlations. These clusters tend to be very massive, have >0.1 dex intrinsic metallicity dispersions, have complex sub-giant branch morphologies, and have correlated [Fe/H] and s-process element enhancements. Interestingly, nearly all of these clusters discovered so far have [Fe/H]~-1.7. In this context, we have examined the chemical composition of 18 red giant branch (RGB) stars in the massive, metal-poor Galactic bulge globular cluster NGC 6273 using high signal-to-noise, high resolution (R~27,000) spectra obtained with the Michigan/Magellan Fiber System (M2FS) and MSpec spectrograph mounted on the Magellan-Clay 6.5m telescope at Las Campanas Observatory. We find that the cluster exhibits a metallicity range from [Fe/H]=-1.80 to -1.30 and is composed of two dominant populations separated in [Fe/H] and [La/Fe] abundance. The increase in [La/Eu] as a function of [La/H] suggests that the increase in [La/Fe] with [Fe/H] is due to almost pure s-process enrichment. The most metal-rich star in our sample is not strongly La-enhanced, but is α-poor and may belong to a third "anomalous" stellar population. The two dominant populations exhibit the same [Na/Fe]-[Al/Fe] correlation found in other "normal" globular clusters. Therefore, NGC 6273 joins ω Centauri, M 22, M 2, and NGC 5286 as a possible new class of Galactic globular clusters.

  20. The SUMO project I. A survey of multiple populations in globular clusters

    Science.gov (United States)

    Monelli, M.; Milone, A. P.; Stetson, P. B.; Marino, A. F.; Cassisi, S.; del Pino Molina, A.; Salaris, M.; Aparicio, A.; Asplund, M.; Grundahl, F.; Piotto, G.; Weiss, A.; Carrera, R.; Cebrián, M.; Murabito, S.; Pietrinferni, A.; Sbordone, L.

    2013-05-01

    We present a general overview and the first results of the SUMO project (a SUrvey of Multiple pOpulations in Globular Clusters). The objective of this survey is the study of multiple stellar populations in the largest sample of globular clusters homogeneously analysed to date. To this aim we obtained high signal-to-noise (S/N > 50) photometry for main sequence stars with mass down to ˜0.5 M⊙ in a large sample of clusters using both archival and proprietary U, B, V and I data from ground-based telescopes. In this paper, we focus on the occurrence of multiple stellar populations in 23 clusters. We define a new photometric index, cU, B, I = (U - B) - (B - I), which turns out to be very effective for identifying multiple sequences along the red giant branch (RGB). We found that in the V-cU, B, I diagram all clusters presented in this paper show broadened or multimodal RGBs, with the presence of two or more components. We found a direct connection with the chemical properties of different sequences, which display different abundances of light elements (O, Na, C, N and Al). The cU, B, I index is also a powerful tool for identifying distinct sequences of stars along the horizontal branch and, for the first time in the case of NGC 104 (47 Tuc), along the asymptotic giant branch. Our results demonstrate that (i) the presence of more than two stellar populations is a common feature amongst globular clusters, as already highlighted in previous work; (ii) multiple sequences with different chemical contents can be easily identified by using standard Johnson photometry obtained with ground-based facilities; (iii) in the study of globular cluster multiple stellar populations the cU, B, I index is an alternative to spectroscopy, and has the advantage of larger statistics.

  1. HST Proper Motions of Distant Globular Clusters: Constraining the Formation & Mass of the Milky Way

    Science.gov (United States)

    Sohn, S. Tony; van der Marel, Roeland P.; Deason, Alis; Bellini, Andrea; Besla, Gurtina; Watkins, Laura

    2018-04-01

    Proper motions (PMs) are required to calculate accurate orbits of globular clusters (GCs) in the Milky Way (MW) halo. We present our HST program to create a PM database for 20 GCs at distances of R GC = 10-100 kpc. Targets are discussed along with PM measurement methods. We also describe how our PM results can be used for Gaia as an external check, and discuss the synergy between HST and Gaia as astrometric instruments in the coming years.

  2. Distribución en gran escala de los cúmulos globulares en Fornax

    Science.gov (United States)

    Ostrov, P. G.

    Para analizar los cúmulos globulares azules y rojos de NGC 1399 asociados con NGC 1399 en particular, o si los cúmulos azules representaban un sistema asociado con el cúmulo de Fornax en general, se obtuvieron imágenes CCD de gran formato con el telescopio de 4m del CTIO, en las bandas C y T1. Se describe el método empleado y lo encontrado.

  3. Storage of the complement components C4, C3, and C 3-activator in the human liver as PAS-negative globular hyaline bodies.

    Science.gov (United States)

    Storch, W; Riedel, H; Trautmann, B; Justus, J; Hiemann, D

    1982-01-01

    Liver biopsies of a 58-year-old clinically healthy patient with a hepatomegaly and intracisternal PAS-negative globular hyaline bodies were immunofluorescent-optically examined for the content of the complement components C 1 q, C 4, C 9, C 1-inactivator, C 3-activator. Further examinations were performed for fibrinogen, IgG, IgA, IgM, IgD, IgE, L-chain (type chi and lambda), alpha 1-antitrypsin, alpha 1-fetoprotein, alpha 1- and alpha 2-glycoprotein, cholinesterase, ceruloplasmin, myoglobin, hemopexin, HBsAg and HBsAg. Th inclusion bodies reacted with antisera against the complement components C 4, C 3 and C 3-activator, as also identified by double immunofluorescence. Probably this is a disturbance of the protein metabolism of the liver cell with abnormal complement storage in the presence of normal total complement and normal complement components in the serum.

  4. Glove powder's carrying capacity for latex protein: analysis using the ASTM ELISA test.

    Science.gov (United States)

    Beezhold, D; Horton, K; Hickey, V; Daddona, J; Kostyal, D

    2003-01-01

    Glove donning powders carry latex proteins and disperse them into the workplace environment. We have used the ASTM D6499 ELISA to quantify the amount of latex antigen bound to and carried by glove powders. We could differentiate between a small amount of protein actually bound to the powders and a larger amount carried by the powder. Enhanced binding of a major allergen, Hev b 5, to the starch powders was demonstrated by Western blot. The D6499 ELISA is able to measure total latex antigen, soluble and powder bound, simultaneously without the need to centrifuge the samples.

  5. Evidence from stellar abundances for a large age difference between two globular clusters

    International Nuclear Information System (INIS)

    Dickens, R.J.; Croke, B.F.W.; Cannon, R.D.; Bell, R.A.

    1991-01-01

    The globular clusters NGC288 and NGC362 are central to recent claims of large age differences (∼3 Gyr) between globular clusters associated with our Galaxy. According to standard models for the formation of the Galaxy, the system of globular clusters formed during the dynamical collapse of the protogalactic cloud, a process which should have lasted no more than 1 Gyr. But the claimed age differences are derived from stellar evolution models using assumed CNO abundances, and uncertainty in the actual CNO abundances of about a factor of three could account for an apparent 2-Gyr age difference. We have accurately measured abundances in red giants in NGC288 and NGC362, and find that the Fe abundance and the sum of the C, N and O abundances are essentially the same in every star studied. By eliminating compositional differences and thus confirming the reality of the age difference, these results imply a cluster formation period that is hard to reconcile with the standard collapse model. (author)

  6. Chemical analysis of eight giant stars of the globular cluster NGC 6366

    Science.gov (United States)

    Puls, Arthur A.; Alves-Brito, Alan; Campos, Fabíola; Dias, Bruno; Barbuy, Beatriz

    2018-05-01

    The metal-rich Galactic globular cluster NGC 6366 is the fifth closest to the Sun. Despite its interest, it has received scarce attention, and little is known about its internal structure. Its kinematics suggests a link to the halo, but its metallicity indicates otherwise. We present a detailed chemical analysis of eight giant stars of NGC 6366, using high-resolution and high-quality spectra (R > 40 000, S/N > 60) obtained at the VLT (8.2 m) and CFHT (3.6 m) telescopes. We attempted to characterize its chemistry and to search for evidence of multiple stellar populations. The atmospheric parameters were derived using the method of excitation and ionization equilibrium of Fe I and Fe II lines and from those atmospheric parameters we calculated the abundances for other elements and found that none of the elements measured presents star-to-star variation greater than the uncertainties. We compared the derived abundances with those of other globular clusters and field stars available in the literature. We determined a mean [Fe/H] = -0.60 ± 0.03 for NGC 6366 and found some similarity of this object with M 71, another inner halo globular cluster. The Na-O anticorrelation extension is short and no star-to-star variation in Al is found. The presence of second generation stars is not evident in NGC 6366.

  7. From Globular Clusters to Tidal Dwarfs: Structure Formation in Tidal Tails

    Science.gov (United States)

    Knierman, K.; Hunsberger, S.; Gallagher, S.; Charlton, J.; Whitmore, B.; Hibbard, J.; Kundu, A.; Zaritsky, D.

    1999-12-01

    Galaxy interactions trigger star formation in tidal debris. How does this star formation depend on the local and global physical conditions? Using WFPC2/HST images, we investigate the range of structure within tidal tails of four classic ``Toomre Sequence'' mergers: NGC 4038/9 (``Antennae''), NGC 7252 (``Atoms for Peace''), NGC 3921, and NGC 3256. These tails contain a variety of stellar associations with sizes from globular clusters up to dwarf Irregulars. We explore whether there is a continuum between the two extremes. Our eight fields sample seven tidal tails at a variety of stages in the evolutionary sequence. Some of these tails are rich in HI while others are HI poor. Large tidal dwarfs are embedded in three of the tails. Using V and I WFPC2 images, we measure luminosities and colors of substructures within the tidal tails. The properties of globular cluster candidates in the tails will be contrasted with those of the hundreds of young clusters in the central regions of these mergers. We address whether globular clusters form and survive in the tidal tails and whether tidal dwarfs are composed of only young stars. By comparing the properties of structures in the tails of the four mergers with different ages, we examine systematic evolution of structure along the evolutionary sequence and as a function of HI content. We acknowledge support from NASA through STScI, and from NSF for an REU supplement for Karen Knierman.

  8. VARIABLE STARS IN LARGE MAGELLANIC CLOUD GLOBULAR CLUSTERS. II. NGC 1786

    International Nuclear Information System (INIS)

    Kuehn, Charles A.; Smith, Horace A.; De Lee, Nathan; Catelan, Márcio; Pritzl, Barton J.; Borissova, Jura

    2012-01-01

    This is the second in a series of papers studying the variable stars in Large Magellanic Cloud globular clusters. The primary goal of this series is to study how RR Lyrae stars in Oosterhoff-intermediate systems compare to their counterparts in Oosterhoff I/II systems. In this paper, we present the results of our new time-series B–V photometric study of the globular cluster NGC 1786. A total of 65 variable stars were identified in our field of view. These variables include 53 RR Lyraes (27 RRab, 18 RRc, and 8 RRd), 3 classical Cepheids, 1 Type II Cepheid, 1 Anomalous Cepheid, 2 eclipsing binaries, 3 Delta Scuti/SX Phoenicis variables, and 2 variables of undetermined type. Photometric parameters for these variables are presented. We present physical properties for some of the RR Lyrae stars, derived from Fourier analysis of their light curves. We discuss several different indicators of Oosterhoff type which indicate that the Oosterhoff classification of NGC 1786 is not as clear cut as what is seen in most globular clusters.

  9. Gravitational microlensing by low-mass objects in the globular cluster M22.

    Science.gov (United States)

    Sahu, K C; Casertano, S; Livio, M; Gilliland, R L; Panagia, N; Albrow, M D; Potter, M

    2001-06-28

    Gravitational microlensing offers a means of determining directly the masses of objects ranging from planets to stars, provided that the distances and motions of the lenses and sources can be determined. A globular cluster observed against the dense stellar field of the Galactic bulge presents ideal conditions for such observations because the probability of lensing is high and the distances and kinematics of the lenses and sources are well constrained. The abundance of low-mass objects in a globular cluster is of particular interest, because it may be representative of the very early stages of star formation in the Universe, and therefore indicative of the amount of dark baryonic matter in such clusters. Here we report a microlensing event associated with the globular cluster M22. We determine the mass of the lens to be 0.13(+0.03)(-0.02) solar masses. We have also detected six events that are unresolved in time. If these are also microlensing events, they imply that a non-negligible fraction of the cluster mass resides in the form of free-floating planetary-mass objects.

  10. No Evidence for Multiple Stellar Populations in the Low-mass Galactic Globular Cluster E 3

    Science.gov (United States)

    Salinas, Ricardo; Strader, Jay

    2015-08-01

    Multiple stellar populations are a widespread phenomenon among Galactic globular clusters. Even though the origin of the enriched material from which new generations of stars are produced remains unclear, it is likely that self-enrichment will be feasible only in clusters massive enough to retain this enriched material. We searched for multiple populations in the low mass (M˜ 1.4× {10}4 {M}⊙ ) globular cluster E3, analyzing SOAR/Goodman multi-object spectroscopy centered on the blue cyanogen (CN) absorption features of 23 red giant branch stars. We find that the CN abundance does not present the typical bimodal behavior seen in clusters hosting multistellar populations, but rather a unimodal distribution that indicates the presence of a genuine single stellar population, or a level of enrichment much lower than in clusters that show evidence for two populations from high-resolution spectroscopy. E3 would be the first bona fide Galactic old globular cluster where no sign of self-enrichment is found. Based on observations obtained at the Southern Astrophysical Research (SOAR) Telescope, which is a joint project of the Ministério da Ciência, Tecnologia, e Inovação (MCTI) da República Federativa do Brasil, the US National Optical Astronomy Observatory (NOAO), the University of North Carolina at Chapel Hill (UNC), and Michigan State University (MSU).

  11. Chemical Abundances of Red Giant Branch Stars in the Globular Clusters NGC 6333 and NGC 6366

    Science.gov (United States)

    Johnson, Christian I.; Rich, R. M.; Pilachowski, C. A.; Kunder, A. M.

    2013-01-01

    We present chemical abundances and radial velocities for >20 red giant branch (RGB) stars in the Galactic globular clusters NGC 6333 ([Fe/H]≈-1.8) and NGC 6366 ([Fe/H]≈-0.6). The results are based on moderate resolution (R=18,000), high signal-to-noise ratio (>100) spectra obtained with the Hydra multifiber positioner and bench spectrograph on the WIYN 3.5m telescope at Kitt Peak National Observatory. Both objects are likely associated with the Galactic bulge globular cluster system, and we therefore compare the cluster abundance patterns with those of nearby bulge field stars. Additionally, we investigate differences in the O-Na anticorrelation and neutron-capture element dispersion between the two clusters, and compare their abundance patterns with those of similar metallicity halo globular clusters. This material is based upon work supported by the National Science Foundation under award No. AST-1003201 to C.I.J. C.A.P. gratefully acknowledges support from the Daniel Kirkwood Research Fund at Indiana University. R.M.R. acknowledges support from NSF grant AST-0709479 and AST-121120995.

  12. Globular Cluster Variable Stars—Atlas and Coordinate Improvement using AAVSOnet Telescopes (Abstract)

    Science.gov (United States)

    Welch, D.; Henden, A.; Bell, T.; Suen, C.; Fare, I.; Sills, A.

    2015-12-01

    (Abstract only) The variable stars of globular clusters have played and continue to play a significant role in our understanding of certain classes of variable stars. Since all stars associated with a cluster have the same age, metallicity, distance and usually very similar (if not identical reddenings), such variables can produce uniquely powerful constraints on where certain types of pulsation behaviors are excited. Advanced amateur astronomers are increasingly well-positioned to provide long-term CCD monitoring of globular cluster variable star but are hampered by a long history of poor or inaccessible finder charts and coordinates. Many of variable-rich clusters have published photographic finder charts taken in relatively poor seeing with blue-sensitive photographic plates. While useful signal-to-noise ratios are relatively straightforward to achieve for RR Lyrae, Type 2 Cepheids, and red giant variables, correct identification remains a difficult issue—particularly when images are taken at V or longer wavelengths. We describe the project and report its progress using the OC61, TMO61, and SRO telescopes of AAVSOnet after the first year of image acquisition and demonstrate several of the data products being developed for globular cluster variables.

  13. A 5.75-millisecond pulsar in the globular cluster 47 Tucanae

    International Nuclear Information System (INIS)

    Manchester, R.N.; Lyne, A.G.; Johnston, S.; D'Amico, N.; Lim, J.; Kniffen, D.A.

    1990-01-01

    Millisecond pulsars are generally believed to be old pulsars that have been spun up ('recycled') as a result of accretion of matter from a companion in a low-mass X-ray binary system. As there is a high incidence of such systems in globular clusters, these are good places to search for millisecond pulsars; so far, ten globular-cluster pulsars have been detected unambiguously. Using the Parkes radiotelescope in Australia, we have found a pulsar with a period of 5.75 ms and a dispersion measure of 25 cm -3 pc in the direction of 47 Tucanae. Despite its probable origin as a member of a binary system, timing measurements show that the pulsar is now single. The observed dispersion measure is consistent with the pulsar lying outside the galactic electron layer and within 47 Tucanae; but it is very different from the value of 67 cm -3 pc for the pulsars that were reported recently as being in this globular cluster, and we suggest that the latter pulsars probably do not in fact lie within 47 Tucanae. (author)

  14. ON THE SERENDIPITOUS DISCOVERY OF A Li-RICH GIANT IN THE GLOBULAR CLUSTER NGC 362

    Energy Technology Data Exchange (ETDEWEB)

    D’Orazi, Valentina; Gratton, Raffaele G.; Lucatello, Sara; Momany, Yazan [INAF—Osservatorio Astronomico di Padova, Vicolo dell’Osservatorio 5, I-35122, Padova (Italy); Angelou, George C. [Max Planck Institut für Sonnensystemforschung, Justus-von-Liebig-Weg 3, D-37077 Göttingen (Germany); Bragaglia, Angela; Carretta, Eugenio; Sollima, Antonio [INAF—Osservatorio Astronomico di Bologna, via Ranzani 1, I-40127, Bologna (Italy); Lattanzio, John C., E-mail: valentina.dorazi@oapd.inaf.it [Monash Centre for Astrophysics (MoCA), Monash University, Melbourne, VIC 3800 (Australia)

    2015-03-10

    We have serendipitously identified the first lithium-rich giant star located close to the red giant branch bump in a globular cluster. Through intermediate-resolution FLAMES spectra we derived a lithium abundance of A(Li) = 2.55 (assuming local thermodynamical equilibrium), which is extremely high considering the star’s evolutionary stage. Kinematic and photometric analysis confirm the object as a member of the globular cluster NGC 362. This is the fourth Li-rich giant discovered in a globular cluster, but is the only one known to exist at a luminosity close to the bump magnitude. The three previous detections are clearly more evolved, located close to, or beyond, the tip of their red giant branch. Our observations are able to discard the accretion of planets/brown dwarfs, as well as an enhanced mass-loss mechanism as a formation channel for this rare object. While the star sits just above the cluster bump luminosity, its temperature places it toward the blue side of the giant branch in the color–magnitude diagram. We require further dedicated observations to unambiguously identify the star as a red giant: we are currently unable to confirm whether Li production has occurred at the bump of the luminosity function or if the star is on the pre-zero-age horizontal branch. The latter scenario provides the opportunity for the star to have synthesized Li rapidly during the core helium flash or gradually during its red giant branch ascent via some extra mixing process.

  15. Study of the spray to globular transition in gas metal arc welding: a spectroscopic investigation

    International Nuclear Information System (INIS)

    Valensi, F; Pellerin, S; Castillon, Q; Zielinska, S; Boutaghane, A; Dzierzega, K; Pellerin, N; Briand, F

    2013-01-01

    The gas metal arc welding (GMAW) process is strongly influenced by the composition of the shielding gas. In particular, addition of CO 2 increases the threshold current for the transition from unstable globular to more stable spray transfer mode. We report on the diagnostics—using optical emission spectroscopy—of a GMAW plasma in pure argon and in mixtures of argon, CO 2 and N 2 while operated in spray and globular transfer modes. The spatially resolved plasma parameters are obtained by applying the Abel transformation to laterally integrated emission data. The Stark widths of some iron lines are used to determine both electron density and temperature, and line intensities yield relative contents of neutral and ionized iron to argon. Our experimental results indicate a temperature drop on the arc axis in the case of spray arc transfer. This drop reduces with addition of N 2 and disappears in globular transfer mode when CO 2 is added. Despite the temperature increase, the electron density decreases with CO 2 concentration. The highest concentration of iron is observed in the plasma column upper part (close to the anode) and for GMAW with CO 2 . Our results are compared with recently published works where the effect of non-homogeneous metal vapour concentration has been taken into account. (paper)

  16. Testing for adaptive evolution of the female reproductive protein ZPC in mammals, birds and fishes reveals problems with the M7-M8 likelihood ratio test.

    Science.gov (United States)

    Berlin, Sofia; Smith, Nick G C

    2005-11-10

    Adaptive evolution appears to be a common feature of reproductive proteins across a very wide range of organisms. A promising way of addressing the evolutionary forces responsible for this general phenomenon is to test for adaptive evolution in the same gene but among groups of species, which differ in their reproductive biology. One can then test evolutionary hypotheses by asking whether the variation in adaptive evolution is consistent with the variation in reproductive biology. We have attempted to apply this approach to the study of a female reproductive protein, zona pellucida C (ZPC), which has been previously shown by the use of likelihood ratio tests (LRTs) to be under positive selection in mammals. We tested for evidence of adaptive evolution of ZPC in 15 mammalian species, in 11 avian species and in six fish species using three different LRTs (M1a-M2a, M7-M8, and M8a-M8). The only significant findings of adaptive evolution came from the M7-M8 test in mammals and fishes. Since LRTs of adaptive evolution may yield false positives in some situations, we examined the properties of the LRTs by several different simulation methods. When we simulated data to test the robustness of the LRTs, we found that the pattern of evolution in ZPC generates an excess of false positives for the M7-M8 LRT but not for the M1a-M2a or M8a-M8 LRTs. This bias is strong enough to have generated the significant M7-M8 results for mammals and fishes. We conclude that there is no strong evidence for adaptive evolution of ZPC in any of the vertebrate groups we studied, and that the M7-M8 LRT can be biased towards false inference of adaptive evolution by certain patterns of non-adaptive evolution.

  17. Very Broad [O III] λλ4959, 5007 Emission from the NGC 4472 Globular Cluster RZ 2109 and Implications for the Mass of Its Black Hole X-Ray Source

    Science.gov (United States)

    Zepf, Stephen E.; Stern, Daniel; Maccarone, Thomas J.; Kundu, Arunav; Kamionkowski, Marc; Rhode, Katherine L.; Salzer, John J.; Ciardullo, Robin; Gronwall, Caryl

    2008-08-01

    We present Keck LRIS spectroscopy of the black hole-hosting globular cluster RZ 2109 in the Virgo elliptical galaxy NGC 4472. We find that this object has extraordinarily broad [O III] λ5007 and [O III] λ4959 emission lines, with velocity widths of approximately 2000 km s-1. This result has significant implications for the nature of this accreting black hole system and the mass of the globular cluster black hole. We show that the broad [O III] λ5007 emission must arise from material driven at high velocity from the black hole system. This is because the volume available near the black hole is too small by many orders of magnitude to have enough [O III]-emitting atoms to account for the observed L([O III] λ5007) at high velocities, even if this volume is filled with oxygen at the critical density for [O III] λ5007. The Balmer emission is also weak, indicating the observed [O III] is not due to shocks. We therefore conclude that the [O III] λλ4959, 5007 is produced by photoionization of material driven across the cluster. The only known way to drive significant material at high velocity is for a system accreting mass near or above its Eddington limit, which indicates a stellar-mass black hole. Since it is dynamically implausible to form an accreting stellar-mass black hole system in a globular cluster with an intermediate-mass black hole (IMBH), it appears this massive globular cluster does not have an IMBH. We discuss further tests of this conclusion, and its implications for the MBH - Mstellar and MBH - σ relations. Based on observations made at the W. M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California, and the National Aeronautics and Space Administration. The Observatory was made possible by the generous financial support of the W. M. Keck Foundation.

  18. Blind testing of routine, fully automated determination of protein structures from NMR data.

    NARCIS (Netherlands)

    Rosato, A.; Aramini, J.M.; Arrowsmith, C.; Bagaria, A.; Baker, D.; Cavalli, A.; Doreleijers, J.; Eletsky, A.; Giachetti, A.; Guerry, P.; Gutmanas, A.; Guntert, P.; He, Y.; Herrmann, T.; Huang, Y.J.; Jaravine, V.; Jonker, H.R.; Kennedy, M.A.; Lange, O.F.; Liu, G.; Malliavin, T.E.; Mani, R.; Mao, B.; Montelione, G.T.; Nilges, M.; Rossi, P.; Schot, G. van der; Schwalbe, H.; Szyperski, T.A.; Vendruscolo, M.; Vernon, R.; Vranken, W.F.; Vries, S.D. de; Vuister, G.W.; Wu, B.; Yang, Y.; Bonvin, A.M.

    2012-01-01

    The protocols currently used for protein structure determination by nuclear magnetic resonance (NMR) depend on the determination of a large number of upper distance limits for proton-proton pairs. Typically, this task is performed manually by an experienced researcher rather than automatically by

  19. Blind Testing of Routine, Fully Automated Determination of Protein Structures from NMR Data

    NARCIS (Netherlands)

    Rosato, A.; Aramini, J.M.; van der Schot, G.; de Vries, S.J.|info:eu-repo/dai/nl/304837717; Bonvin, A.M.J.J.|info:eu-repo/dai/nl/113691238

    2012-01-01

    The protocols currently used for protein structure determination by nuclear magnetic resonance (NMR) depend on the determination of a large number of upper distance limits for proton-proton pairs. Typically, this task is performed manually by an experienced researcher rather than automatically by

  20. Quantifying Protein Concentrations Using Smartphone Colorimetry: A New Method for an Established Test

    Science.gov (United States)

    Gee, Clifford T.; Kehoe, Eric; Pomerantz, William C. K.; Penn, R. Lee

    2017-01-01

    Proteins are involved in nearly every biological process, which makes them of interest to a range of scientists. Previous work has shown that hand-held cameras can be used to determine the concentration of colored analytes in solution, and this paper extends the approach to reactions involving a color change in order to quantify protein…

  1. Contribution to the study of proteins and peptides structure by hydrogen isotopic exchange

    International Nuclear Information System (INIS)

    Nabedryk-Viala, Eliane.

    1978-01-01

    Development of hydrogen exchange measurement methods to study the structure and the molecular interaction of globular protein molecules in aqueous solution (ribonuclease A, cytochrome c, coupling factors of chloroplasts), in peptide hormones in trifluoroethanol solution (angiotensin II, corticotropin) and in proteins of membranes (rhodopsin) [fr

  2. Trigonellae Semen Enhances Sperm Motility and the Expression of the Cation Sperm Channel Proteins in Mouse Testes

    Directory of Open Access Journals (Sweden)

    Do Rim Kim

    2015-01-01

    Full Text Available Genetic defects during spermatogenesis can lead to a reduction in sperm motility and cause male infertility. The cation channels of sperm (CatSper play a role in the regulation of hyperactivated sperm motility in mouse testes. The effect of Trigonellae Semen (TS on the male reproductive system and CatSper protein in mouse testes during spermatogenesis was examined. C57BL/c mice were divided into the following five groups: normal, cyclophosphamide- (CP- only treated (control group, and three groups treated with varying concentrations of TS with CP (100, 500, and 1000 mg/kg TS and 100 mg/kg CP. Real-time PCR, western blot analysis, and a testosterone immunoassay were performed to assess CatSper protein levels in the five groups. Additionally, sperm cell counts and motility were examined. Results indicate that sperm motility and sperm counts increased in the TS treated groups in a dose-dependent manner (p<0.01. CatSper levels were also significantly higher in the TS treated groups compared to that of the control group (p<0.001. Therefore, TS treatment could enhance sperm function by promoting spermatogenesis and the expression of CatSper proteins in mouse testes.

  3. Chemical abundances in the globular clusters NGC6229 and NGC6779

    Science.gov (United States)

    Khamidullina, D. A.; Sharina, M. E.; Shimansky, V. V.; Davoust, E.

    2014-10-01

    Long-slit medium-resolution spectra of the Galactic globular clusters (GCs) NGC6229 and NGC6779, obtained with the CARELEC spectrograph at the 1.93-m telescope of the Haute-Provence observatory, have been used to determine the age, helium abundance (Y), and metallicity [Fe/H] as well as the first estimate of the abundances of C, N, O, Mg, Ca, Ti, and Cr for these objects. We solved this task by comparing the observed spectra and the integrated synthetic spectra, calculated with the use of the stellar atmosphere models with the parameters preset for the stars from these clusters. The model mass estimates, T eff, and log g were derived by comparing the observed "color-magnitude" diagrams and the theoretical isochrones. The summing-up of the synthetic blanketed stellar spectra was conducted according to the Chabrier mass function. To test the accuracy of the results, we estimated the chemical abundances, [Fe/H], log t, and Y for the NGC5904 and NGC6254 clusters, which, according to the literature, are considered to be the closest analogues of the two GCs of our study. Using the medium-resolution spectra from the library of Schiavon et al., we obtained for these two clusters a satisfactory agreement with the reported estimates for all the parameters within the errors. We derived the following cluster parameters. NGC6229: [Fe/H] = -1.65 dex, t = 12.6 Gyr, Y = 0.26, [ α/Fe] = 0.28 dex; NGC6779: [Fe/H] = -1.9 dex, t = 12.6 Gyr, Y = 0.23, [ α/Fe] = 0.08 dex; NGC5904: [Fe/H] = -1.6 dex, t = 12.6 Gyr, Y = 0.30, [ α/Fe] = 0.35 dex; NGC6254: [Fe/H] = -1.52 dex, t = 11.2 Gyr, Y = 0.30, [ α/Fe] = 0.025 dex. The value [ α/Fe] denotes the average of the Ca and Mg abundances.

  4. Detailed abundances from integrated-light spectroscopy: Milky Way globular clusters

    Science.gov (United States)

    Larsen, S. S.; Brodie, J. P.; Strader, J.

    2017-05-01

    Context. Integrated-light spectroscopy at high spectral resolution is rapidly maturing as a powerful way to measure detailed chemical abundances for extragalactic globular clusters (GCs). Aims: We test the performance of our analysis technique for integrated-light spectra by applying it to seven well-studied Galactic GCs that span a wide range of metallicities. Methods: Integrated-light spectra were obtained by scanning the slit of the UVES spectrograph on the ESO Very Large Telescope across the half-light diameters of the clusters. We modelled the spectra using resolved Hubble Space Telescope colour-magnitude diagrams (CMDs), as well as theoretical isochrones, in combination with standard stellar atmosphere and spectral synthesis codes. The abundances of Fe, Na, Mg, Ca, Ti, Cr, and Ba were compared with literature data for individual stars in the clusters. Results: The typical differences between iron abundances derived from our integrated-light spectra and those compiled from the literature are less than 0.1 dex. A larger difference is found for one cluster (NGC 6752), and is most likely caused primarily by stochastic fluctuations in the numbers of bright red giants within the scanned area. As expected, the α-elements (Ca, Ti) are enhanced by about 0.3 dex compared to the Solar-scaled composition, while the [Cr/Fe] ratios are close to Solar. When using up-to-date line lists, our [Mg/Fe] ratios also agree well with literature data. Our [Na/Fe] ratios are, on average, 0.08-0.14 dex lower than average values quoted in the literature, and our [Ba/Fe] ratios may be overestimated by 0.20-0.35 dex at the lowest metallicities. We find that analyses based on theoretical isochrones give very similar results to those based on resolved CMDs. Conclusions: Overall, the agreement between our integrated-light abundance measurements and the literature data is satisfactory. Refinements of the modelling procedure, such as corrections for stellar evolutionary and non-LTE effects

  5. Recombinant Protein Truncation Strategy for Inducing Bactericidal Antibodies to the Macrophage Infectivity Potentiator Protein of Neisseria meningitidis and Circumventing Potential Cross-Reactivity with Human FK506-Binding Proteins

    OpenAIRE

    Bielecka, Magdalena K.; Devos, Nathalie; Gilbert, Mélanie; Hung, Miao-Chiu; Weynants, Vincent; Heckels, John E.; Christodoulides, Myron

    2014-01-01

    A recombinant macrophage infectivity potentiator (rMIP) protein of Neisseria meningitidis induces significant serum bactericidal antibody production in mice and is a candidate meningococcal vaccine antigen. However, bioinformatics analysis of MIP showed some amino acid sequence similarity to human FK506-binding proteins (FKBPs) in residues 166 to 252 located in the globular domain of the protein. To circumvent the potential concern over generating antibodies that could recognize human protein...

  6. Strategy to Identify and Test Putative Light-Sensitive Non-Opsin G-Protein-Coupled Receptors: A Case Study.

    Science.gov (United States)

    Faggionato, Davide; Serb, Jeanne M

    2017-08-01

    The rise of high-throughput RNA sequencing (RNA-seq) and de novo transcriptome assembly has had a transformative impact on how we identify and study genes in the phototransduction cascade of non-model organisms. But the advantage provided by the nearly automated annotation of RNA-seq transcriptomes may at the same time hinder the possibility for gene discovery and the discovery of new gene functions. For example, standard functional annotation based on domain homology to known protein families can only confirm group membership, not identify the emergence of new biochemical function. In this study, we show the importance of developing a strategy that circumvents the limitations of semiautomated annotation and apply this workflow to photosensitivity as a means to discover non-opsin photoreceptors. We hypothesize that non-opsin G-protein-coupled receptor (GPCR) proteins may have chromophore-binding lysines in locations that differ from opsin. Here, we provide the first case study describing non-opsin light-sensitive GPCRs based on tissue-specific RNA-seq data of the common bay scallop Argopecten irradians (Lamarck, 1819). Using a combination of sequence analysis and three-dimensional protein modeling, we identified two candidate proteins. We tested their photochemical properties and provide evidence showing that these two proteins incorporate 11-cis and/or all-trans retinal and react to light photochemically. Based on this case study, we demonstrate that there is potential for the discovery of new light-sensitive GPCRs, and we have developed a workflow that starts from RNA-seq assemblies to the discovery of new non-opsin, GPCR-based photopigments.

  7. Tracing dust in old stellar populations : the mid-infrared spectrum of globular cluster AGB stars

    International Nuclear Information System (INIS)

    Richter, H.

    2010-01-01

    Asymptotic Giant Branch (AGB) stars are considered to be the main stellar dust producers in the universe. Their dusty circumstellar shells leave fingerprints in the mid-infrared (MIR) spectra of AGB stars and in unresolved old stellar populations. Bressan et al. (2007) showed that co-added MIR-spectra of AGB stars of known luminosity, metallicity and age (like those found in the Galactic globular cluster NGC104) can be used to model the dust excess in early-type galaxies. This work aims to improve our understanding of the MIR-spectra of old stellar populations with respect to their metallicities by studying a large sample of AGB stars in Galactic globular clusters. A sample of AGB stars (taken from Lebzelter et al. 2006 and Sloan et al. 2010) is used to produce co-added MIR-spectra of globular cluster combinations for three metallicity groups. Each group consists of several globular clusters with similar age and metallicity. Combining the clusters leads to a higher number of AGB stars with available Spitzer spectra in each group. The low metallicity group (Z=0.0038) consists of five globular clusters with 18 AGB star spectra, the intermediate (Z=0.0058) and high (Z=0.01) metallicity groups both include three clusters with eight and seven available MIR-spectra, respectively. Stars within the 90% mass radius of each globular cluster are used to generate 2MASS Color- Magnitude diagrams (CMDs) of each cluster combination. Binning the stars in the CMDs with respect to their MK-values results in Luminosity Functions (LFs) for the cluster combinations. The LFs based on 2MASS data are compared to LFs obtained using theoretical isochrones from the Padova group (Bertelli et al. 2008, Marigo et al. 2008). Using the 2MASS LFs integrated MIR-spectra of the three globular cluster combinations are derived by weighting the existing spectra with the total number of AGB stars within each MK-bin of the LFs along the upper giant branch. This relies on the assumption that stars that

  8. Blood serum components and serum protein test of Hybro-PG broilers of different ages

    Directory of Open Access Journals (Sweden)

    PRL Silva

    2007-12-01

    Full Text Available Blood serum samples of HYBRO PG broilers were analyzed, with 30 samples collected from 21-day-old broilers (G1, 30 from 35-day-old birds (G2, and 30 from 42-day-old birds (G3, with the aim of establishing normal values of some blood serum parameters. The activities of the enzymes gamma-glutamyl-transferase (GGT, aspartate aminotransferase (AST, creatine kinase (CK, alkaline phosphatase (ALP, and lactate dehydrogenase (LDH, serum levels of total calcium, calcium ion, phosphorus, sodium, potassium, magnesium, chlorides, creatinine, uric acid, triglycerides, cholesterol, total protein, albumin, total and indirect and direct bilirubin, and electrophoretic profile of serum proteins in acrylamide (SDS-PAGE and agarose gel were determined. There was no influence of age on total bilirubin and albumin levels. All the other evaluated parameters presented differences in at least one age group. Protein electrophoretic profile also changed as a function of age. The obtained results can be considered as normal for the studied ages, and therefore be used as references for the interpretation of laboratory exams of broilers of this genetic line in the evaluated ages.

  9. Structural test of the parameterized-backbone method for protein design.

    Science.gov (United States)

    Plecs, Joseph J; Harbury, Pehr B; Kim, Peter S; Alber, Tom

    2004-09-03

    Designing new protein folds requires a method for simultaneously optimizing the conformation of the backbone and the side-chains. One approach to this problem is the use of a parameterized backbone, which allows the systematic exploration of families of structures. We report the crystal structure of RH3, a right-handed, three-helix coiled coil that was designed using a parameterized backbone and detailed modeling of core packing. This crystal structure was determined using another rationally designed feature, a metal-binding site that permitted experimental phasing of the X-ray data. RH3 adopted the intended fold, which has not been observed previously in biological proteins. Unanticipated structural asymmetry in the trimer was a principal source of variation within the RH3 structure. The sequence of RH3 differs from that of a previously characterized right-handed tetramer, RH4, at only one position in each 11 amino acid sequence repeat. This close similarity indicates that the design method is sensitive to the core packing interactions that specify the protein structure. Comparison of the structures of RH3 and RH4 indicates that both steric overlap and cavity formation provide strong driving forces for oligomer specificity.

  10. Free Fatty Acid Concentration and Carboxy methyl cellulase Activity of Some Formulas of Protected Fat-proteins Tested In Vitro

    Directory of Open Access Journals (Sweden)

    Lilis Hartati

    2015-05-01

    Full Text Available The aim of this study was to determine the levels of free fatty acids and carboxymethylcellulase activity (cmc-ase activity of some protected fat-proteins base on in vitro Tilley and Terry method. Two sources of fat, i.e. crude palm oil and fish oil and three sources of protein i.e. skim milk, soybean flour and soybean meal were used in the formulation of protected fat-protein, and thus there were six treatment combinations. The filtrate from the in vitro test was analyzed for the levels of free fatty acids and  cmcase activity. The result of this research indicates that different combinations of feed materials and fat give different content of free fatty acid in first stage and second stage in vitro, with the best results in the combination treatment of skim milk and palm oil that give the lowest result of  free fatty acid concentration in fisrt stage in vitro (0.168% and the highest result free fatty acid concentration in second stage in vitro ( 4.312% . The activity of CMC-ase was not influenced by different  sources of fat and protein. It can be concluded was that the protection of the combination between skim milk and CPO gives the highest protection results.

  11. The nature of the progenitor of the M31 north-western stream: globular clusters as milestones of its orbit

    Science.gov (United States)

    Kirihara, T.; Miki, Y.; Mori, M.

    2017-08-01

    We examine the nature, possible orbits and physical properties of the progenitor of the north-western stellar stream (NWS) in the halo of the Andromeda galaxy (M31). The progenitor is assumed to be an accreting dwarf galaxy with globular clusters (GCs). It is, in general, difficult to determine the progenitor's orbit precisely because of many necessary parameters. Recently, Veljanoski et al. reported five GCs whose positions and radial velocities suggest an association with the stream. We use these data to constrain the orbital motions of the progenitor using test-particle simulations. Our simulations split the orbit solutions into two branches according to whether the stream ends up in the foreground or in the background of M31. Upcoming observations that will determine the distance to the NWS will be able to reject one of the two branches. In either case, the solutions require that the pericentric radius of any possible orbit be over 2 kpc. We estimate the efficiency of the tidal disruption and confirm the consistency with the assumption for the progenitor being a dwarf galaxy. The progenitor requires the mass ≳ 2 × 106 M⊙ and half-light radius ≳ 30 pc. In addition, N-body simulations successfully reproduce the basic observed features of the NWS and the GCs' line-of-sight velocities.

  12. DERIVING METALLICITIES FROM THE INTEGRATED SPECTRA OF EXTRAGALACTIC GLOBULAR CLUSTERS USING THE NEAR-INFRARED CALCIUM TRIPLET

    International Nuclear Information System (INIS)

    Foster, Caroline; Forbes, Duncan A.; Proctor, Robert N.; Spitler, Lee R.; Strader, Jay; Brodie, Jean P.

    2010-01-01

    The Ca II triplet (CaT) feature in the near-infrared has been employed as a metallicity indicator for individual stars as well as integrated light of Galactic globular clusters (GCs) and galaxies with varying degrees of success, and sometimes puzzling results. Using the DEIMOS multi-object spectrograph on Keck we obtain a sample of 144 integrated light spectra of GCs around the brightest group galaxy NGC 1407 to test whether the CaT index can be used as a metallicity indicator for extragalactic GCs. Different sets of single stellar population models make different predictions for the behavior of the CaT as a function of metallicity. In this work, the metallicities of the GCs around NGC 1407 are obtained from CaT index values using an empirical conversion. The measured CaT/metallicity distributions show unexpected features, the most remarkable being that the brightest red and blue GCs have similar CaT values despite their large difference in mean color. Suggested explanations for this behavior in the NGC 1407 GC system are (1) the CaT may be affected by a population of hot blue stars, (2) the CaT may saturate earlier than predicted by the models, and/or (3) color may not trace metallicity linearly. Until these possibilities are understood, the use of the CaT as a metallicity indicator for the integrated spectra of extragalactic GCs will remain problematic.

  13. Hemoglobin interactions with αB crystallin: a direct test of sensitivity to protein instability.

    Directory of Open Access Journals (Sweden)

    Tyler J W Clark

    Full Text Available As a small stress response protein, human αB crystallin, detects protein destabilization that can alter structure and function to cause self assembly of fibrils or aggregates in diseases of aging. The sensitivity of αB crystallin to protein instability was evaluated using wild-type hemoglobin (HbA and hemoglobin S (HbS, the glutamate-6-valine mutant that forms elongated, filamentous aggregates in sickling red blood cells. The progressive thermal unfolding and aggregation of HbA and HbS in solution at 37°C, 50°C and 55°C was measured as increased light scattering. UV circular dichroism (UVCD was used to evaluate conformational changes in HbA and HbS with time at the selected temperatures. The changes in interactions between αB crystallin and HbA or HbS with temperature were analyzed using differential centrifugation and SDS PAGE at 37°C, 50°C and 55°C. After only 5 minutes at the selected temperatures, differences in the aggregation or conformation of HbA and HbS were not observed, but αB crystallin bound approximately 6% and 25% more HbS than HbA at 37°C, and 50°C respectively. The results confirmed (a the remarkable sensitivity of αB crystallin to structural instabilities at the very earliest stages of thermal unfolding and (b an ability to distinguish the self assembling mutant form of HbS from the wild type HbA in solution.

  14. Systematic Testing of Belief-Propagation Estimates for Absolute Free Energies in Atomistic Peptides and Proteins.

    Science.gov (United States)

    Donovan-Maiye, Rory M; Langmead, Christopher J; Zuckerman, Daniel M

    2018-01-09

    Motivated by the extremely high computing costs associated with estimates of free energies for biological systems using molecular simulations, we further the exploration of existing "belief propagation" (BP) algorithms for fixed-backbone peptide and protein systems. The precalculation of pairwise interactions among discretized libraries of side-chain conformations, along with representation of protein side chains as nodes in a graphical model, enables direct application of the BP approach, which requires only ∼1 s of single-processor run time after the precalculation stage. We use a "loopy BP" algorithm, which can be seen as an approximate generalization of the transfer-matrix approach to highly connected (i.e., loopy) graphs, and it has previously been applied to protein calculations. We examine the application of loopy BP to several peptides as well as the binding site of the T4 lysozyme L99A mutant. The present study reports on (i) the comparison of the approximate BP results with estimates from unbiased estimators based on the Amber99SB force field; (ii) investigation of the effects of varying library size on BP predictions; and (iii) a theoretical discussion of the discretization effects that can arise in BP calculations. The data suggest that, despite their approximate nature, BP free-energy estimates are highly accurate-indeed, they never fall outside confidence intervals from unbiased estimators for the systems where independent results could be obtained. Furthermore, we find that libraries of sufficiently fine discretization (which diminish library-size sensitivity) can be obtained with standard computing resources in most cases. Altogether, the extremely low computing times and accurate results suggest the BP approach warrants further study.

  15. Use of test day milk fat and milk protein to detect subclinical ketosis in dairy cattle in Ontario.

    Science.gov (United States)

    Duffield, T F; Kelton, D F; Leslie, K E; Lissemore, K D; Lumsden, J H

    1997-01-01

    Serum beta-hydroxybutyrate (BHB) levels were determined for 1333 dairy cows in various stages of lactation and parity on 93 dairy farms in Ontario. The data were collected in a cross-sectional manner, as part of the 1992 Ontario Dairy Monitoring and Analysis Program. The median serum BHB was 536 mumol/L for all cows, with a range of 0 to 5801 mumol/L. When subclinical ketosis was defined as a serum BHB level of 1200 mumol/L or higher, the prevalence of ketosis for cows in early lactation ( 149 DIM), and dry cows were 5.3%, 3.2%, and 1.6%, respectively. The mean serum BHB was significantly higher in the early group compared with each of the other 3 groups (P ketosis. However, test-day fat percent and test-day protein percent, used alone or in combination, were not useful screening tests for identifying cows with subclinical ketosis. PMID:9360791

  16. C-reactive protein testing in patients with acute rhinosinusitis leads to a reduction in antibiotic use

    DEFF Research Database (Denmark)

    Llor, Carl; Bjerrum, Lars; Arranz, Javier

    2012-01-01

    OBJECTIVE: To evaluate the effect of C-reactive protein (CRP) testing on the antibiotic prescribing in patients with acute rhinosinusitis. METHODS: Audit-based study carried out in primary care centres in Spain. GPs registered episodes of rhinosinusitis during 3-week period before and after...... an intervention. Two types of intervention were considered: full intervention group (FIG) consisting in individual feedback based on results from the first registry, courses in rational antibiotic prescribing, guidelines, patient information leaflets, workshops on rapid tests and use of the CRP test. GPs...... in the partial intervention group (PIG) underwent all the above intervention except for the workshop and they did not have access to CRP. Multilevel logistic regression analysis was performed considering the prescription of antibiotics as the dependent variable. RESULTS: Two hundred and ten physicians were...

  17. Correlating labeling chemistry and in-vitro test results with the biological behavior of radiolabeled proteins

    International Nuclear Information System (INIS)

    Srivastava, S.C.; Meinken, G.E.

    1985-01-01

    Monoclonal antibodies possess enormous potential for delivery of therapeutic amounts of radionuclides to target antigens in vivo, in particular for tumor imaging and therapy. Translation of this concept into practice has encountered numerous problems. Specifically whereas general protein radiolabeling methods are applicable to antibodies, immunological properties of the antibodies are often compromised resulting in reduced in-vivo specificity for the target antigens. The bifunctional chelating agent approach shows the most promise, however, development of other agents will be necessary for widespread usefulness of this technique. The effects of labeling chemistry on the in-vivo behavior of several monoclonal antibodies are described. 30 refs., 4 figs., 10 tabs

  18. Probing amyloid protein aggregation with optical superresolution methods: from the test tube to models of disease.

    Science.gov (United States)

    Kaminski, Clemens F; Kaminski Schierle, Gabriele S

    2016-10-01

    The misfolding and self-assembly of intrinsically disordered proteins into insoluble amyloid structures are central to many neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. Optical imaging of this self-assembly process in vitro and in cells is revolutionizing our understanding of the molecular mechanisms behind these devastating conditions. In contrast to conventional biophysical methods, optical imaging and, in particular, optical superresolution imaging, permits the dynamic investigation of the molecular self-assembly process in vitro and in cells, at molecular-level resolution. In this article, current state-of-the-art imaging methods are reviewed and discussed in the context of research into neurodegeneration.

  19. Solution structure of the human signaling protein RACK1

    Directory of Open Access Journals (Sweden)

    Papa Priscila F

    2010-06-01

    Full Text Available Abstract Background The adaptor protein RACK1 (receptor of activated kinase 1 was originally identified as an anchoring protein for protein kinase C. RACK1 is a 36 kDa protein, and is composed of seven WD repeats which mediate its protein-protein interactions. RACK1 is ubiquitously expressed and has been implicated in diverse cellular processes involving: protein translation regulation, neuropathological processes, cellular stress, and tissue development. Results In this study we performed a biophysical analysis of human RACK1 with the aim of obtaining low resolution structural information. Small angle X-ray scattering (SAXS experiments demonstrated that human RACK1 is globular and monomeric in solution and its low resolution structure is strikingly similar to that of an homology model previously calculated by us and to the crystallographic structure of RACK1 isoform A from Arabidopsis thaliana. Both sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation techniques showed that RACK1 is predominantly a monomer of around 37 kDa in solution, but also presents small amounts of oligomeric species. Moreover, hydrodynamic data suggested that RACK1 has a slightly asymmetric shape. The interaction of RACK1 and Ki-1/57 was tested by sedimentation equilibrium. The results suggested that the association between RACK1 and Ki-1/57(122-413 follows a stoichiometry of 1:1. The binding constant (KB observed for RACK1-Ki-1/57(122-413 interaction was of around (1.5 ± 0.2 × 106 M-1 and resulted in a dissociation constant (KD of (0.7 ± 0.1 × 10-6 M. Moreover, the fluorescence data also suggests that the interaction may occur in a cooperative fashion. Conclusion Our SAXS and analytical ultracentrifugation experiments indicated that RACK1 is predominantly a monomer in solution. RACK1 and Ki-1/57(122-413 interact strongly under the tested conditions.

  20. pH dependence of the kinetics of interfacial tension changes during protein adsorption from sessile droplets on FEP-Teflon

    NARCIS (Netherlands)

    VanderVegt, W; Norde, W; VanderMei, HC; Busscher, HJ

    Interfacial tension changes during protein adsorption at both the solid-liquid and the liquid-vapor interface were measured simultaneously by ADSA-P from sessile droplets of protein solutions on fluoroethylenepropylene-Teflon. Four globular proteins of similar size, viz. lysozyme, ribonuclease,

  1. Protein fiber linear dichroism for structure determination and kinetics in a low-volume, low-wavelength couette flow cell.

    Science.gov (United States)

    Dafforn, Timothy R; Rajendra, Jacindra; Halsall, David J; Serpell, Louise C; Rodger, Alison

    2004-01-01

    High-resolution structure determination of soluble globular proteins relies heavily on x-ray crystallography techniques. Such an approach is often ineffective for investigations into the structure of fibrous proteins as these proteins generally do not crystallize. Thus investigations into fibrous protein structure have relied on less direct methods such as x-ray fiber diffraction and circular dichroism. Ultraviolet linear dichroism has the potential to provide additional information on the structure of such biomolecular systems. However, existing systems are not optimized for the requirements of fibrous proteins. We have designed and built a low-volume (200 microL), low-wavelength (down to 180 nm), low-pathlength (100 microm), high-alignment flow-alignment system (couette) to perform ultraviolet linear dichroism studies on the fibers formed by a range of biomolecules. The apparatus has been tested using a number of proteins for which longer wavelength linear dichroism spectra had already been measured. The new couette cell has also been used to obtain data on two medically important protein fibers, the all-beta-sheet amyloid fibers of the Alzheimer's derived protein Abeta and the long-chain assemblies of alpha1-antitrypsin polymers.

  2. The structure of the Myo4p globular tail and its function in ASH1 mRNA localization.

    Science.gov (United States)

    Heuck, Alexander; Fetka, Ingrid; Brewer, Daniel N; Hüls, Daniela; Munson, Mary; Jansen, Ralf-Peter; Niessing, Dierk

    2010-05-03

    Type V myosin (MyoV)-dependent transport of cargo is an essential process in eukaryotes. Studies on yeast and vertebrate MyoV showed that their globular tails mediate binding to the cargo complexes. In Saccharomyces cerevisiae, the MyoV motor Myo4p interacts with She3p to localize asymmetric synthesis of HO 1 (ASH1) mRNA into the bud of dividing cells. A recent study showed that localization of GFP-MS2-tethered ASH1 particles does not require the Myo4p globular tail, challenging the supposed role of this domain. We assessed ASH1 mRNA and Myo4p distribution more directly and found that their localization is impaired in cells expressing globular tail-lacking Myo4p. In vitro studies further show that the globular tail together with a more N-terminal linker region is required for efficient She3p binding. We also determined the x-ray structure of the Myo4p globular tail and identify a conserved surface patch important for She3p binding. The structure shows pronounced similarities to membrane-tethering complexes and indicates that Myo4p may not undergo auto-inhibition of its motor domain.

  3. Diagnosis of protein-losing gastroenteropathy by sup 111 In-transferrin scanning and fecal excretion test

    Energy Technology Data Exchange (ETDEWEB)

    Urita, Yoshihisa (Toho Univ., Tokyo (Japan). School of Medicine)

    1991-10-01

    A total of 12 patients with gastroenteropathy were studied for possible protein loss. The underlying diseases were intestinal lymphangiectasia, ulcerative colitis and Crohn's disease in 2 patients each and Menetrier's disease, Cronkhite-Canada syndrome, postgastrectomy syndrome, severe atrophic gastritis, gastric cancer and gastroenteropathy of unknown origin in one patient each. The controls were four healthy volunteers and 9 patients with benign disease not accompanied by lesions in the digestive tract and protein loss {sup 111}In-transferrin Tf was prepared by incubating 2-3 mCi of {sup 111}In-chloride with 15-20 ml of each patient's plasma in a sterilized vial at room temperature for 1 hour. After iv injection of {sup 111}In-Tf the patients were scanned at given intervals of time for 72 hours to localize protein loss. Seventy-two hour fecal {sup 111}In-Tf excretion was also measured. {sup 111}In-Tf was detected in the feces of all patients. The recovery rate in the patient group was 7.95{+-}3.65%, being significantly higher than 0.48{+-}0.26% in the control group. However {sup 111}In-Tf scanning showed the site of protein loss only in 6 patients. Negative scan in six other patients was thought to be associated with extensive inflammation and severe diarrhea. Five of the 12 patients were observed before and after treatment. A decrease in fecal {sup 111}In-Tf excretion with clinical improvement was noted in the 5 patients after treatment. The site of protein loss could be demonstrated in 3 of the 5 patients before treatment. Its site in 1 of the 3 patients was the small intestine. However accumulation of {sup 111}In-Tf was found there in the 3 patients after treatment. The site of protein loss in 1 of 3 patients was the small intestine. {sup 111}In-Tf scanning is particularly useful when the small intestine is involved, because surgery is contraindicated in this case. Fecal {sup 111}In-Tf excretion test is mandatory when the site of protein loss

  4. Meals based on vegetable protein sources (beans and peas) are more satiating than meals based on animal protein sources (veal and pork) – a randomized cross-over meal test study

    Science.gov (United States)

    Kristensen, Marlene D.; Bendsen, Nathalie T.; Christensen, Sheena M.; Astrup, Arne; Raben, Anne

    2016-01-01

    Background Recent nutrition recommendations advocate a reduction in protein from animal sources (pork, beef) because of environmental concerns. Instead, protein from vegetable sources (beans, peas) should be increased. However, little is known about the effect of these vegetable protein sources on appetite regulation. Objective To examine whether meals based on vegetable protein sources (beans/peas) are comparable to meals based on animal protein sources (veal/pork) regarding meal-induced appetite sensations. Design In total, 43 healthy, normal-weight, young men completed this randomized, double-blind, placebo-controlled, three-way, cross-over meal test. The meals (all 3.5 MJ, 28 energy-% (E%) fat) were either high protein based on veal and pork meat, HP-Meat (19 E% protein, 53 E% carbohydrate, 6 g fiber/100 g); high protein based on legumes (beans and peas), HP-Legume (19 E% protein, 53 E% carbohydrate, 25 g fiber/100 g); or low-protein based on legumes, LP-Legume (9 E% protein, 62 E% carbohydrate, 10 g fiber/100 g). Subjective appetite sensations were recorded at baseline and every half hour using visual analog scales until the ad libitum meal 3 h after the test meal. Repeated measurements analyses and summary analyses were performed using ANCOVA (SAS). Results HP-Legume induced lower composite appetite score, hunger, prospective food consumption, and higher fullness compared to HP-Meat and LP-Legume (ppeas) influenced appetite sensations favorably compared to animal-based meals (pork/veal) with similar energy and protein content, but lower fiber content. Interestingly, a vegetable-based meal with low protein content was as satiating and palatable as an animal-based meal with high protein content. PMID:27765144

  5. Physics of protein folding

    Science.gov (United States)

    Finkelstein, A. V.; Galzitskaya, O. V.

    2004-04-01

    Protein physics is grounded on three fundamental experimental facts: protein, this long heteropolymer, has a well defined compact three-dimensional structure; this structure can spontaneously arise from the unfolded protein chain in appropriate environment; and this structure is separated from the unfolded state of the chain by the “all-or-none” phase transition, which ensures robustness of protein structure and therefore of its action. The aim of this review is to consider modern understanding of physical principles of self-organization of protein structures and to overview such important features of this process, as finding out the unique protein structure among zillions alternatives, nucleation of the folding process and metastable folding intermediates. Towards this end we will consider the main experimental facts and simple, mostly phenomenological theoretical models. We will concentrate on relatively small (single-domain) water-soluble globular proteins (whose structure and especially folding are much better studied and understood than those of large or membrane and fibrous proteins) and consider kinetic and structural aspects of transition of initially unfolded protein chains into their final solid (“native”) 3D structures.

  6. Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression

    Directory of Open Access Journals (Sweden)

    Perera Rajika L

    2004-12-01

    Full Text Available Abstract Background In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote high yields of soluble protein. Proteins studied included cell surface receptors (Ephrins and Eph receptors, CD44, kinases (EGFR-cytoplasmic domain, CDK2 and 4, proteases (MMP1, CASP2, signal transduction proteins (GRB2, RAF1, HRAS and transcription factors (GATA2, Fli1, Trp53, Mdm2, JUN, FOS, MAD, MAX. Over 400 experiments were performed where expression of 30 full-length proteins and protein domains were evaluated with 6 different N-terminal and 8 C-terminal fusion partners. Expression of an additional set of 95 mammalian proteins was also performed to test the conclusions of this study. Results Several protein features correlated with soluble protein expression yield including molecular weight and the number of contiguous hydrophobic residues and low complexity regions. There was no relationship between successful expression and protein pI, grand average of hydropathicity (GRAVY, or sub-cellular location. Only small globular cytoplasmic proteins with an average molecular weight of 23 kDa did not require a solubility enhancing tag for high level soluble expression. Thioredoxin (Trx and maltose binding protein (MBP were the best N-terminal protein fusions to promote soluble expression, but MBP was most effective as a C-terminal fusion. 63 of 95 mammalian proteins expressed at soluble levels of greater than 1 mg/l as N-terminal H10-MBP fusions and those that failed possessed, on average, a higher molecular weight and greater number of contiguous hydrophobic amino acids and low complexity regions. Conclusions By analysis of the protein features identified here, this study will help predict which mammalian proteins and domains can be successfully expressed in E. coli as soluble product and also which are best targeted for a eukaryotic expression system. In some cases

  7. Analysis of long-range correlation in sequences data of proteins

    OpenAIRE

    ADRIANA ISVORAN; LAURA UNIPAN; DANA CRACIUN; VASILE MORARIU

    2007-01-01

    The results presented here suggest the existence of correlations in the sequence data of proteins. 32 proteins, both globular and fibrous, both monomeric and polymeric, were analyzed. The primary structures of these proteins were treated as time series. Three spatial series of data for each sequence of a protein were generated from numerical correspondences between each amino acid and a physical property associated with it, i.e., its electric charge, its polar character and its dipole moment....

  8. New cataclysmic variables and other exotic binaries in the globular cluster 47 Tucanae*

    Science.gov (United States)

    Rivera Sandoval, L. E.; van den Berg, M.; Heinke, C. O.; Cohn, H. N.; Lugger, P. M.; Anderson, J.; Cool, A. M.; Edmonds, P. D.; Wijnands, R.; Ivanova, N.; Grindlay, J. E.

    2018-04-01

    We present 22 new (+3 confirmed) cataclysmic variables (CVs) in the non-core-collapsed globular cluster 47 Tucanae (47 Tuc). The total number of CVs in the cluster is now 43, the largest sample in any globular cluster so far. For the identifications we used near-ultraviolet (NUV) and optical images from the Hubble Space Telescope, in combination with X-ray results from the Chandra X-ray Observatory. This allowed us to build the deepest NUV CV luminosity function of the cluster to date. We found that the CVs in 47 Tuc are more concentrated towards the cluster centre than the main-sequence turn-off stars. We compared our results to the CV populations of the core-collapsed globular clusters NGC 6397 and NGC 6752. We found that 47 Tuc has fewer bright CVs per unit mass than those two other clusters. That suggests that dynamical interactions in core-collapsed clusters play a major role creating new CVs. In 47 Tuc, the CV population is probably dominated by primordial and old dynamically formed systems. We estimated that the CVs in 47 Tuc have total masses of ˜1.4 M⊙. We also found that the X-ray luminosity function of the CVs in the three clusters is bimodal. Additionally, we discuss a possible double degenerate system and an intriguing/unclassified object. Finally, we present four systems that could be millisecond pulsar companions given their X-ray and NUV/optical colours. For one of them we present very strong evidence for being an ablated companion. The other three could be CO or He white dwarfs.

  9. BVRI main-sequence photometry of the globular cluster M4

    International Nuclear Information System (INIS)

    Alcaino, G.; Liller, W.

    1984-01-01

    We present BV and RI photographic photometry of 1421 and 189 stars, respectively, in the intermediate metallicity globular cluster M4 (NGC 6121). This investigation includes the first results of RI main-sequence photometry of a globular cluster. The use of longer wavelengths and longer color baselines provides the potential of improved isochrone fittings and underscores the urgent need for calculations of RI synthetic isochrones to be compared with observations. The Pickering-Racine wedge was used with the ESO 3.6 m telescope, the Las Campanas 2.5 m du Pont telescope, and the CTIO 1 m Yale telescope to extend the photoelectric limit from Vroughly-equal16.1 to Vroughly-equal19.1. We have determined the position of the main-sequence turnoff to lie at V = 16.6 +- 0.2 (m.e.) and B-V = 0.80 +- 0.03 (m.e.). A comparison of our BV observations with the CCD data of Richer and Fahlman shows excellent agreement: the two fifucial main sequences agree at all points to within 0.025 mag and, on average, to 0.013 mag. For the cluster we derive a distance modulus (m-M)/sub V/ = 12.52 +- 0.2 and reddening E(B-V) = 0.44 +- 0.03, results which confirm that at a distance of 2 kpc, M4 is the closest globular clusters to the Sun. Using the isochrones of VandenBerg, we deduce an age 13 +- 2 Gyr. As noted in several other investigations, there is a striking deficiency of stars in certain parts of the color-magnitude diagram; in M4 we find a pronounced gap over approx.0.6 mag at the base of the subgiant branch

  10. Hubble Space Telescope Observations of cD Galaxies and Their Globular Cluster Systems

    Science.gov (United States)

    Jordán, Andrés; Côté, Patrick; West, Michael J.; Marzke, Ronald O.; Minniti, Dante; Rejkuba, Marina

    2004-01-01

    We have used WFPC2 on the Hubble Space Telescope (HST) to obtain F450W and F814W images of four cD galaxies (NGC 541 in Abell 194, NGC 2832 in Abell 779, NGC 4839 in Abell 1656, and NGC 7768 in Abell 2666) in the range 5400 km s-1cluster (GC) systems reveals no anomalies in terms of specific frequencies, metallicity gradients, average metallicities, or the metallicity offset between the globular clusters and the host galaxy. We show that the latter offset appears roughly constant at Δ[Fe/H]~0.8 dex for early-type galaxies spanning a luminosity range of roughly 4 orders of magnitude. We combine the globular cluster metallicity distributions with an empirical technique described in a series of earlier papers to investigate the form of the protogalactic mass spectrum in these cD galaxies. We find that the observed GC metallicity distributions are consistent with those expected if cD galaxies form through the cannibalism of numerous galaxies and protogalactic fragments that formed their stars and globular clusters before capture and disruption. However, the properties of their GC systems suggest that dynamical friction is not the primary mechanism by which these galaxies are assembled. We argue that cD's instead form rapidly, via hierarchical merging, prior to cluster virialization. Based on observations with the NASA/ESA Hubble Space Telescope obtained at the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-26555 Based in part on observations obtained at the European Southern Observatory, for VLT program 68.D-0130(A).

  11. Variable Stars in Large Magellanic Cloud Globular Clusters. II. NGC 1786

    Science.gov (United States)

    Kuehn, Charles A.; Smith, Horace A.; Catelan, Márcio; Pritzl, Barton J.; De Lee, Nathan; Borissova, Jura

    2012-12-01

    This is the second in a series of papers studying the variable stars in Large Magellanic Cloud globular clusters. The primary goal of this series is to study how RR Lyrae stars in Oosterhoff-intermediate systems compare to their counterparts in Oosterhoff I/II systems. In this paper, we present the results of our new time-series B-V photometric study of the globular cluster NGC 1786. A total of 65 variable stars were identified in our field of view. These variables include 53 RR Lyraes (27 RRab, 18 RRc, and 8 RRd), 3 classical Cepheids, 1 Type II Cepheid, 1 Anomalous Cepheid, 2 eclipsing binaries, 3 Delta Scuti/SX Phoenicis variables, and 2 variables of undetermined type. Photometric parameters for these variables are presented. We present physical properties for some of the RR Lyrae stars, derived from Fourier analysis of their light curves. We discuss several different indicators of Oosterhoff type which indicate that the Oosterhoff classification of NGC 1786 is not as clear cut as what is seen in most globular clusters. Based on observations taken with the SMARTS 1.3 m telescope operated by the SMARTS Consortium and observations taken at the Southern Astrophysical Research (SOAR) telescope, which is a joint project of the Ministério da Ciência, Tecnologia, e Inovação (MCTI) da República Federativa do Brasil, the U.S. National Optical Astronomy Observatory (NOAO), the University of North Carolina at Chapel Hill (UNC), and Michigan State University (MSU).

  12. Covariance functions across herd production levels for test day records on milk, fat, and protein yields

    NARCIS (Netherlands)

    Veerkamp, R.F.; Goddard, M.E.

    1998-01-01

    Multiple-trait BLUP evaluations of test day records require a large number of genetic parameters. This study estimated covariances with a reduced model that included covariance functions in two dimensions (stage of lactation and herd production level) and all three yield traits. Records came from

  13. Problem-Solving Test: Nucleocytoplasmic Shuttling of Pre-mRNA Binding Proteins

    Science.gov (United States)

    Szeberenyi, Jozsef

    2012-01-01

    Terms to be familiar with before you start to solve the test: transcription, pre-mRNA, RNA processing, RNA transport, RNA polymerase II, direct and indirect immunofluorescence staining, cell fractionation by centrifugation, oligo(dT)-cellulose chromatography, washing and elution of the column, ribonuclease, SDS-polyacrylamide gel electrophoresis,…

  14. ALMA reveals sunburn: CO dissociation around AGB stars in the globular cluster 47 Tucanae

    OpenAIRE

    McDonald, Iain; Zijlstra, Albert A.; Lagadec, Eric; Sloan, Gregory C.; Boyer, Martha L.; Matsuura, Mikako; Smith, Rowan J.; Smith, Christina L.; Yates, Jeremy A.; van Loon, Jacco Th.; Jones, Olivia C.; Ramstedt, Sofia; Avison, Adam; Justtanont, Kay; Olofsson, Hans

    2015-01-01

    Atacama Large Millimetre Array observations show a non-detection of carbon monoxide around the four most luminous asymptotic giant branch (AGB) stars in the globular cluster 47 Tucanae. Stellar evolution models and star counts show that the mass-loss rates from these stars should be similar to 1.2-3.5x10(-7) M-circle dot yr(-1). We would naively expect such stars to be detectable at this distance (4.5 kpc). By modelling the ultraviolet radiation field from post-AGB stars and white dwarfs in 4...

  15. M31 GLOBULAR CLUSTER STRUCTURES AND THE PRESENCE OF X-RAY BINARIES

    International Nuclear Information System (INIS)

    Agar, J. R. R.; Barmby, P.

    2013-01-01

    The Andromeda galaxy, M31, has several times the number of globular clusters found in the Milky Way. It contains a correspondingly larger number of low-mass X-ray binaries (LMXBs) associated with globular clusters, and as such can be used to investigate the cluster properties that lead to X-ray binary formation. The best tracer of the spatial structure of M31 globulars is the high-resolution imaging available from the Hubble Space Telescope (HST), and we have used HST data to derive structural parameters for 29 LMXB-hosting M31 globular clusters. These measurements are combined with structural parameters from the literature for a total of 41 (of 50 known) LMXB clusters and a comparison sample of 65 non-LMXB clusters. Structural parameters measured in blue bandpasses are found to be slightly different (smaller core radii and higher concentrations) than those measured in red bandpasses; this difference is enhanced in LMXB clusters and could be related to stellar population differences. Clusters with LMXBs show higher collision rates for their mass compared to clusters without LMXBs, and collision rates estimated at the core radius show larger offsets than rates estimated at the half-light radius. These results are consistent with the dynamical formation scenario for LMXBs. A logistic regression analysis finds that, as expected, the probability of a cluster hosting an LMXB increases with increasing collision rate and proximity to the galaxy center. The same analysis finds that probability of a cluster hosting an LMXB decreases with increasing cluster mass at a fixed collision rate, although we caution that this could be due to sample selection effects. Metallicity is found to be a less important predictor of LMXB probability than collision rate, mass, or distance, even though LMXB clusters have a higher metallicity on average. This may be due to the interaction of location and metallicity: a sample of M31 LMXBs with a greater range in galactocentric distance would

  16. Deep and accurate near-infrared photometry of the Galactic globular cluster omega Cen .

    Science.gov (United States)

    Calamida, A.; Bono, G.; Corsi, C. E.; Stetson, P. B.; Prada Moroni, P. G.; Degl'Innocenti, S.; Marchetti, E.; Amico, P.; Ferraro, I.; Iannicola, G.; Monelli, M.; Buonanno, R.; Caputo, F.; Dall'Ora, M.; Freyhammer, L. M.; Koester, D.; Nonino, M.; Piersimoni, A. M.; Pulone, L.; Romaniello, M.

    We present deep and accurate Near-Infrared (NIR) photometry of the Galactic Globular Cluster omega Cen . Data were collected using the Multi-Conjugate Adaptive Optics Demonstrator (MAD) mounted on the VLT (ESO). We combined the NIR photometry with optical space data collected with the Advanced Camera for Surveys (ACS) for the same region of the cluster. Our deep optical-NIR CMD indicates that the spread in age among the different stellar populations in omega Cen is at most of the order of 2 Gyr.

  17. On the radial distribution of white dwarfs in the Galactic globular cluster omega Cen

    Science.gov (United States)

    Calamida, A.; Corsi, C. E.; Bono, G.; Stetson, P. B.; Prada Moroni, P. G.; Degl'Innocenti, S.; Ferraro, I.; Iannicola, G.; Koester, D.; Pulone, L.; Monelli, M.; Amico, P.; Buonanno, R.; Freyhammer, L. M.; Marchetti, E.; Nonino, M.; Romaniello, M.

    We present deep and accurate photometry (F435W, F625W, F658N) of the Galactic Globular Cluster omega Cen collected with the Advanced Camera for Surveys (ACS) on board the Hubble Space Telescope (HST). We identified ≈ 6,500 white dwarf (WD) candidates and compared their radial distribution with that of Main Sequence (MS) stars. We found a mild evidence that young WDs ( 0.1 ≲ t ≲ 0.6 Gyr) are less centrally concentrated when compared to MS stars in the magnitude range 25 < F435W < 26.5.

  18. Modeling and Analysis of a Spectrum of the Globular Cluster NGC 2419

    OpenAIRE

    Sharina, M. E.; Shimansky, V. V.; Davoust, E.

    2012-01-01

    NGC 2419 is the most distant massive globular cluster in the outer Galactic halo. It is unusual also due to the chemical peculiarities found in its red giant stars in recent years. We study the stellar population of this unusual object using spectra obtained at the 1.93-m telescope of the Haute-Provence Observatory. At variance with commonly used methods of high-resolution spectroscopy applicable only to bright stars, we employ spectroscopic information on the integrated light of the cluster....

  19. The gravitational wave emission from white dwarf interactions in globular clusters

    International Nuclear Information System (INIS)

    Loren-Aguilar, P; Garcia-Berro, E; Lobo, J A; Isern, J

    2009-01-01

    In the dense central regions of globular clusters close encounters of two white dwarfs are relatively frequent. The estimated frequency is one or more strong encounters per star in the lifetime of the cluster. Such encounters should be then potential sources of gravitational wave radiation. Thus, it is foreseeable that these collisions could be either individually detected by LISA or they could contribute significantly to the background noise of the detector. We compute the pattern of gravitational wave emission from these encounters for a sufficiently broad range of system parameters, namely the masses, the relative velocities and the distances of the two white dwarfs involved in the encounter.

  20. Cytotoxicity Test Based on Human Cells Labeled with Fluorescent Proteins: Fluorimetry, Photography, and Scanning for High-Throughput Assay.

    Science.gov (United States)

    Kalinina, Marina A; Skvortsov, Dmitry A; Rubtsova, Maria P; Komarova, Ekaterina S; Dontsova, Olga A

    2018-06-01

    High- and medium-throughput assays are now routine methods for drug screening and toxicology investigations on mammalian cells. However, a simple and cost-effective analysis of cytotoxicity that can be carried out with commonly used laboratory equipment is still required. The developed cytotoxicity assays are based on human cell lines stably expressing eGFP, tdTomato, mCherry, or Katushka2S fluorescent proteins. Red fluorescent proteins exhibit a higher signal-to-noise ratio, due to less interference by medium autofluorescence, in comparison to green fluorescent protein. Measurements have been performed on a fluorescence scanner, a plate fluorimeter, and a camera photodocumentation system. For a 96-well plate assay, the sensitivity per well and the measurement duration were 250 cells and 15 min for the scanner, 500 cells and 2 min for the plate fluorimeter, and 1000 cells and less than 1 min for the camera detection. These sensitivities are similar to commonly used MTT (tetrazolium dye) assays. The used scanner and the camera had not been previously applied for cytotoxicity evaluation. An image processing scheme for the high-resolution scanner is proposed that significantly diminishes the number of control wells, even for a library containing fluorescent substances. The suggested cytotoxicity assay has been verified by measurements of the cytotoxicity of several well-known cytotoxic drugs and further applied to test a set of novel bacteriotoxic compounds in a medium-throughput format. The fluorescent signal of living cells is detected without disturbing them and adding any reagents, thus allowing to investigate time-dependent cytotoxicity effects on the same sample of cells. A fast, simple and cost-effective assay is suggested for cytotoxicity evaluation based on mammalian cells expressing fluorescent proteins and commonly used laboratory equipment.

  1. X-ray tests of microfocusing mono-capillary optic for protein crystallography

    CERN Document Server

    Bilderback, D H

    2001-01-01

    A single, borosilicate-glass capillary was drawn into a 30.5 cm long elliptical shape. The inside diameter was 0.40 mm at the large base end and 0.13 mm at the tip. With 12 keV X-rays from the CHESS D1 bending magnet, the single-bounce capillary produced a focus of better than 18 mu m in diameter (FHWM) at a 3 cm distance from the capillary tip. A flux gain of 110 in the focus position was observed along with a total flux in the spot of 4x10 sup 1 sup 0 X-rays/s (conditions: 5.3 GeV, 182 mA, 1.5% bandwidth multilayer, 12 keV X-rays). A measurement of the far field focus ring diameter yielded a divergence of 3.8 mrad, in good agreement with the 4 mrad design of the optic for protein crystallography. Using a small 25 mu m square beam, we measured the local reflectivity to be greater than 95% and the inner slope errors of the capillary to average about +-150 mu rad, both from raw and elliptically shaped tubing. Our conclusion is that more perfect starting tubing (i.e. one with lower slope errors) is needed to ma...

  2. The globularization hypothesis of the language-ready brain as a developmental frame for prosodic bootstrapping theories of language acquisition

    Directory of Open Access Journals (Sweden)

    Aritz eIrurtzun

    2015-12-01

    Full Text Available In recent research Boeckx & Benítez-Burraco (2014a,b have advanced the hypothesis that our species-specific language-ready brain should be understood as the outcome of developmental changes that occurred in our species after the split from Neanderthals-Denisovans, which resulted in a more globular braincase configuration in comparison to our closest relatives, who had elongated endocasts. According to these authors, the development of a globular brain is an essential ingredient for the language faculty and in particular, it is the centrality occupied by the thalamus in a globular brain that allows its modulatory or regulatory role, essential for syntactico-semantic computations. Their hypothesis is that the syntactico-semantic capacities arise in humans as a consequence of a process of globularization, which significantly takes place postnatally (cf. Neubauer et al. (2010. In this paper, I show that Boeckx & Benítez-Burraco’s hypothesis makes an interesting developmental prediction regarding the path of language acquisition: it teases apart the onset of phonological acquisition and the onset of syntactic acquisition (the latter starting significantly later, after globularization. I argue that this hypothesis provides a developmental rationale for the prosodic bootstrapping hypothesis of language acquisition (cf. i.a. Gleitman & Wanner (1982; Mehler et al. (1988, et seq.; Gervain & Werker (2013, which claim that prosodic cues are employed for syntactic parsing. The literature converges in the observation that a large amount of such prosodic cues (in particular, rhythmic cues are already acquired before the completion of the globularization phase, which paves the way for the premises of prosodic bootstrapping hypothesis, allowing babies to have a rich knowledge of the prosody of their target language before they can start parsing the primary linguistic data syntactically.

  3. The "Globularization Hypothesis" of the Language-ready Brain as a Developmental Frame for Prosodic Bootstrapping Theories of Language Acquisition.

    Science.gov (United States)

    Irurtzun, Aritz

    2015-01-01

    In recent research (Boeckx and Benítez-Burraco, 2014a,b) have advanced the hypothesis that our species-specific language-ready brain should be understood as the outcome of developmental changes that occurred in our species after the split from Neanderthals-Denisovans, which resulted in a more globular braincase configuration in comparison to our closest relatives, who had elongated endocasts. According to these authors, the development of a globular brain is an essential ingredient for the language faculty and in particular, it is the centrality occupied by the thalamus in a globular brain that allows its modulatory or regulatory role, essential for syntactico-semantic computations. Their hypothesis is that the syntactico-semantic capacities arise in humans as a consequence of a process of globularization, which significantly takes place postnatally (cf. Neubauer et al., 2010). In this paper, I show that Boeckx and Benítez-Burraco's hypothesis makes an interesting developmental prediction regarding the path of language acquisition: it teases apart the onset of phonological acquisition and the onset of syntactic acquisition (the latter starting significantly later, after globularization). I argue that this hypothesis provides a developmental rationale for the prosodic bootstrapping hypothesis of language acquisition (cf. i.a. Gleitman and Wanner, 1982; Mehler et al., 1988, et seq.; Gervain and Werker, 2013), which claim that prosodic cues are employed for syntactic parsing. The literature converges in the observation that a large amount of such prosodic cues (in particular, rhythmic cues) are already acquired before the completion of the globularization phase, which paves the way for the premises of the prosodic bootstrapping hypothesis, allowing babies to have a rich knowledge of the prosody of their target language before they can start parsing the primary linguistic data syntactically.

  4. Efficiency of histidine rich protein II-based rapid diagnostic tests for monitoring malaria transmission intensities in an endemic area

    Science.gov (United States)

    Modupe, Dokunmu Titilope; Iyabo, Olasehinde Grace; Oladoke, Oladejo David; Oladeji, Olanrewaju; Abisola, Akinbobola; Ufuoma, Adjekukor Cynthia; Faith, Yakubu Omolara; Humphrey, Adebayo Abiodun

    2018-04-01

    In recent years there has been a global decrease in the prevalence of malaria due to scaling up of control measures, hence global control efforts now target elimination and eradication of the disease. However, a major problem associated with elimination is asymptomatic reservoir of infection especially in endemic areas. This study aims to determine the efficiency of histidine rich protein II (HRP-2) based rapid diagnostic tests (RDT) for monitoring transmission intensities in an endemic community in Nigeria during the pre-elimination stage. Plasmodium falciparum asymptomatic malaria infection in healthy individuals and symptomatic cases were detected using HRP-2. RDT negative tests were re-checked by microscopy and by primer specific PCR amplification of merozoite surface protein 2 (msp-2) for asexual parasites and Pfs25 gene for gametocytes in selected samples to detect low level parasitemia undetectable by microscopy. The mean age of the study population (n=280) was 6.12 years [95% CI 5.16 - 7.08, range 0.5 - 55], parasite prevalence was 44.6% and 36.3% by microscopy and RDT respectively (p =0.056). The parasite prevalence of 61.5% in children aged >2 - 10 years was significantly higher than 3.7% rate in adults >18years (p malaria in endemic areas.

  5. The combination of heteroduplex analysis and protein truncation test for exact detection of the APC gene mutations

    International Nuclear Information System (INIS)

    Tomka, M.; Kirchhoff, T.; Stefurkova, V.; Zajac, V.; Kulcsar, L.

    1998-01-01

    Familial adenomatous polyposis (FAP) is usually associated with mutation in the adenomatous polyposis coli (APC) gene. To examine the occurrence of these mutations in the number of FAP suspected families from the whole Slovakia effectively, we have applied heteroduplex analysis (HDA) and protein truncation test (PTT) for the analyses of 2-5 base pair deletions and point mutations of the APC gene. In the analyzed exon 15 of the APC gene determined by the primers 15Efor-15Grev for HDA and 15ET7-15J3 for PTT more than 70% of mutations should be deletions [3, 12], which are detectable by HDA. In our collection of 5 FAP families mutations in the APC gene were found in families 10, 27 and 41 using HDA. By PTT test the formation of truncated APC protein in FAP families 2, 10, 16 and 27 were revealed. The necessity of combination of at least HDA and PTT techniques for exact detection of APC mutations in analyzed APC region is discussed. (authors)

  6. Testing and analysis on total protein, albumin and A/G of salivary in radiation exposure persons

    International Nuclear Information System (INIS)

    Wang Jun; Zhang Yan; Li Guangwen; Li Gang; Guo Jing; Li Hui; Wang Yuxin; Li Cuixia

    2012-01-01

    Objective: To study the oral health effect of long term low dose radiation on exposure personnel and to provide a basis for further improving the protection ability. Methods: Testing method, which was based on APT and HSA interactions induced by synchronous fluorescence specific changes, and intensity and concentrations of HSA in the solution in the system of synchronous fluorescence showed a good linear relations. the establishment of a APT as a molecular probe was used to test concentration of salivary total protein (TP), albumin (ALB), globulin (GLO) and albumin by synchronous fluorescence spectrum analysis. The information was analyzed in Foxpro 6.0 and SPSS 16.0 software. Result: Protein (TP) Mean Value was 3.904 ±1.369 g/L, Minimum Value was 0.30 g/L and Maximum Value was 7.50 g/L. Albumin (ALB) Mean Value was 0.965±0.665 g/L, Minimum Value was 0.09 g/L and Maximum Value was 3.98 g/L. Globulin (GLO) Mean Value was 2.895±0.947 g/L, Minimum Value was 0.01 g/L and Maximum Value was 5.81 g/L. A/G Mean Value was 0.327. Conclusion: Long term and low dose of radiation would break the chronic physiological balance and concentration of salivary total protein (TP), albumin (ALB), globulin (GLO) and albumin and globulin ratio (A/G) changed obviously. It was necessary to do more special oral health care, further improve the individual protection consciousness, strengthen the radiation monitoring and protection measures, improve the regulation system, and reduce radiation damage on special personnel health significantly. (authors)

  7. Experimental and clinical studies on simultaneous fat and protein tolerance digestion-absorption test using 131I-triolein and 125I-RISA

    International Nuclear Information System (INIS)

    Tokura, Yasunobu

    1979-01-01

    Simultaneous fat and protein tolerance digestion-absorption test using 131 I-triolein and 125 I-RISA was investigated experimentally in rats and clinically in 148 inpatients with various diseases. The results were as follows: Animal experiments. Fat absorption was markedly impaired in the exclusion of bile from the intestine. Each function of fat and protein absorption was independently detected. These animal experiments gave the clinical evaluation of this test for fat and protein digestion-absorption function. Clinical investigations. The test meal was prepared without using commercial Tween 80 as an emulgent. Either fat or protein absorption was markedly impaired in some patients with various diseases. Inpatients with various diseases investigated were divided into 3 groups by correlation coefficient between fecal excretion ratios of fat and protein. Each function of fat and protein digestion-absorption was able to be detected independently by this test simultaneously. This test can further be used as a labolatory aid in evaluating fat and protein digestion-absorption function in patients with malabsorption syndrome. (author)

  8. Synthetic horizontal branch models for globular clusters - the luminosity of the horizontal branch and the Oosterhoff effect

    International Nuclear Information System (INIS)

    Lee, Y.W.; Demarque, P.; Zinn, R.

    1987-01-01

    The variation of horizontal-branch (HB) luminosities with metal abundances is analyzed on the basis of HB models synthesized from theoretical HB evolutionary tracks. The focus is on the Oosterhoff effect, as related to period shifts in globular-cluster RR Lyr variables. The construction of the models and the Oosterhoff period groups is explained in detail, and the implications for globular-cluster ages are considered. The ratio of Delta M(bol) (RR) to Delta Fe/H for the HB is calculated as 0.24, slightly steeper than that found by Sandage (1981 and 1982). 35 references

  9. Foaming and adsorption behavior of bovine and camel proteins mixed layers at the air/water interface.

    Science.gov (United States)

    Lajnaf, Roua; Picart-Palmade, Laetitia; Attia, Hamadi; Marchesseau, Sylvie; Ayadi, M A

    2017-03-01

    The aim of this work was to examine foaming and interfacial behavior of three milk protein mixtures, bovine α-lactalbumin-β-casein (M1), camel α-lactalbumin-β-casein (M2) and β-lactoglobulin-β-casein (M3), alone and in binary mixtures, at the air/water interface in order to better understand the foaming properties of bovine and camel milks. Different mixture ratios (100:0; 75:25; 50:50; 25:75; 0:100) were used during foaming tests and interfacial protein interactions were studied with a pendant drop tensiometer. Experimental results evidenced that the greatest foam was obtained with a higher β-casein amount in all camel and bovine mixtures. Good correlation was observed with the adsorption and the interfacial rheological properties of camel and bovine protein mixtures. The proteins adsorbed layers are mainly affected by the presence of β-casein molecules, which are probably the most abundant protein at interface and the most efficient in reducing the interfacial properties. In contrast of, the globular proteins, α-lactalbumin and β-lactoglobulin that are involved in the protein layer composition, but could not compact well at the interface to ensure foams creation and stabilization because of their rigid molecular structure. Copyright © 2016 Elsevier B.V. All rights reserved.

  10. Cost-effectiveness of point-of-care C-reactive protein testing to inform antibiotic prescribing decisions

    Science.gov (United States)

    Oppong, Raymond; Jit, Mark; Smith, Richard D; Butler, Christopher C; Melbye, Hasse; Mölstad, Sigvard; Coast, Joanna

    2013-01-01

    Background Point-of-care C-reactive protein (POCCRP) is a biomarker of inflammation that offers clinicians a rapid POC test to guide antibiotic prescribing decisions for acute cough and lower respiratory tract infections (LRTI). However, evidence that POCCRP is cost-effective is limited, particularly outside experimental settings. Aim To assess the cost-effectiveness of POCCRP as a diagnostic tool for acute cough and LRTI from the perspective of the health service. Design and setting Observational study of the presentation, management, and outcomes of patients with acute cough and LRTI in primary care settings in Norway and Sweden. Method Using hierarchical regression, data were analysed in terms of the effect on antibiotic use, cost, and patient outcomes (symptom severity after 7 and 14 days, time to recovery, and EQ-5D), while controlling for patient characteristics (self-reported symptom severity, comorbidities, and health-related quality of life) at first attendance. Results POCCRP testing is associated with non-significant positive reductions in antibiotic prescribing (P = 0.078) and increased cost (P = 0.092). Despite the uncertainty, POCCRP testing is also associated with a cost per quality-adjusted life year (QALY) gain of €9391. At a willingness-to-pay threshold of €30 000 per QALY gained, there is a 70% probability of CRP being cost-effective. Conclusion POCCRP testing is likely to provide a cost-effective diagnostic intervention both in terms of reducing antibiotic prescribing and in terms of QALYs gained. PMID:23834883

  11. A Visual Language for Protein Design

    KAUST Repository

    Cox, Robert Sidney

    2017-02-08

    As protein engineering becomes more sophisticated, practitioners increasingly need to share diagrams for communicating protein designs. To this end, we present a draft visual language, Protein Language, that describes the high-level architecture of an engineered protein with easy-to draw glyphs, intended to be compatible with other biological diagram languages such as SBOL Visual and SBGN. Protein Language consists of glyphs for representing important features (e.g., globular domains, recognition and localization sequences, sites of covalent modification, cleavage and catalysis), rules for composing these glyphs to represent complex architectures, and rules constraining the scaling and styling of diagrams. To support Protein Language we have implemented an extensible web-based software diagram tool, Protein Designer, that uses Protein Language in a

  12. A Visual Language for Protein Design

    KAUST Repository

    Cox, Robert Sidney; McLaughlin, James Alastair; Grunberg, Raik; Beal, Jacob; Wipat, Anil; Sauro, Herbert M.

    2017-01-01

    As protein engineering becomes more sophisticated, practitioners increasingly need to share diagrams for communicating protein designs. To this end, we present a draft visual language, Protein Language, that describes the high-level architecture of an engineered protein with easy-to draw glyphs, intended to be compatible with other biological diagram languages such as SBOL Visual and SBGN. Protein Language consists of glyphs for representing important features (e.g., globular domains, recognition and localization sequences, sites of covalent modification, cleavage and catalysis), rules for composing these glyphs to represent complex architectures, and rules constraining the scaling and styling of diagrams. To support Protein Language we have implemented an extensible web-based software diagram tool, Protein Designer, that uses Protein Language in a

  13. The social role of C-reactive protein point-of-care testing to guide antibiotic prescription in Northern Thailand.

    Science.gov (United States)

    Haenssgen, Marco J; Charoenboon, Nutcha; Althaus, Thomas; Greer, Rachel C; Intralawan, Daranee; Lubell, Yoel

    2018-04-01

    New and affordable point-of-care testing (POCT) solutions are hoped to guide antibiotic prescription and to help limit antimicrobial resistance (AMR)-especially in low- and middle-income countries where resource constraints often prevent extensive diagnostic testing. Anthropological and sociological research has illuminated the role and impact of rapid point-of-care malaria testing. This paper expands our knowledge about the social implications of non-malarial POCT, using the case study of a C-reactive-protein point-of-care testing (CRP POCT) clinical trial with febrile patients at primary-care-level health centres in Chiang Rai province, northern Thailand. We investigate the social role of CRP POCT through its interactions with (a) the healthcare workers who use it, (b) the patients whose routine care is affected by the test, and (c) the existing patient-health system linkages that might resonate or interfere with CRP POCT. We conduct a thematic analysis of data from 58 purposively sampled pre- and post-intervention patients and healthcare workers in August 2016 and May 2017. We find widespread positive attitudes towards the test among patients and healthcare workers. Patients' views are influenced by an understanding of CRP POCT as a comprehensive blood test that provides specific diagnosis and that corresponds to notions of good care. Healthcare workers use the test to support their negotiations with patients but also to legitimise ethical decisions in an increasingly restrictive antibiotic policy environment. We hypothesise that CRP POCT could entail greater patient adherence to recommended antibiotic treatment, but it could also encourage riskier health behaviour and entail potentially adverse equity implications for patients across generations and socioeconomic strata. Our empirical findings inform the clinical literature on increasingly propagated point-of-care biomarker tests to guide antibiotic prescriptions, and we contribute to the anthropological and

  14. THE DYNAMICAL EFFECTS OF WHITE DWARF BIRTH KICKS IN GLOBULAR STAR CLUSTERS

    International Nuclear Information System (INIS)

    Fregeau, John M.; Richer, Harvey B.; Rasio, Frederic A.; Hurley, Jarrod R.

    2009-01-01

    Recent observations of the white dwarf (WD) populations in the Galactic globular cluster NGC 6397 suggest that WDs receive a kick of a few km s -1 shortly before they are born. Using our Monte Carlo cluster evolution code, which includes accurate treatments of all relevant physical processes operating in globular clusters, we study the effects of the kicks on their host cluster and on the WD population itself. We find that in clusters whose velocity dispersion is comparable to the kick speed, WD kicks are a significant energy source for the cluster, prolonging the initial cluster core contraction phase significantly so that at late times the cluster core-to-half-mass radius ratio is a factor of up to ∼10 larger than in the no-kick case. WD kicks thus represent a possible resolution of the large discrepancy between observed and theoretically predicted values of this key structural parameter. Our modeling also reproduces the observed trend for younger WDs to be more extended in their radial distribution in the cluster than older WDs.

  15. THE CENTRAL BLUE STRAGGLER POPULATION IN FOUR OUTER-HALO GLOBULAR CLUSTERS

    Energy Technology Data Exchange (ETDEWEB)

    Beccari, Giacomo; Luetzgendorf, Nora [European Southern Observatory, Karl-Schwarzschild-Strasse 2, 85748 Garching bei Muenchen (Germany); Olczak, Christoph [Astronomisches Rechen-Institut (ARI), Zentrum fuer Astronomie Universitaet Heidelberg, Moenchhofstrasse 1214, 69120 Heidelberg (Germany); Ferraro, Francesco R.; Lanzoni, Barbara [Dipartimento di Astronomia, Universita degli Studi di Bologna, via Ranzani 1, I-40127 Bologna (Italy); Carraro, Giovanni; Boffin, Henri M. J. [European Southern Observatory, Alonso de Cordova 3107, Santiago de Chile (Chile); Stetson, Peter B. [National Research Council of Canada, Herzberg Institute of Astrophysics, 5071 West Saanich Road, Victoria, BC V9E 2E7 (Canada); Sollima, Antonio [INAF-Osservatorio Astronomico di Padova, Vicolo dell' Osservatorio 5, 35122 Padova (Italy)

    2012-08-01

    Using Hubble Space Telescope/Wide Field Planetary Camera 2 data, we have performed a comparative study of the Blue Straggler Star (BSS) populations in the central regions of the globular clusters (GCs) AM 1, Eridanus, Palomar 3, and Palomar 4. Located at distances R{sub GC} > 50 kpc from the Galactic center, these are (together with Palomar 14 and NGC 2419) the most distant clusters in the halo. We determine their color-magnitude diagrams and centers of gravity. The four clusters turn out to have similar ages (10.5-11 Gyr), significantly smaller than those of the inner-halo globulars, and similar metallicities. By exploiting wide-field ground-based data, we build the most extended radial density profiles from resolved star counts ever published for these systems. These are well reproduced by isotropic King models of relatively low concentration. BSSs appear to be significantly more centrally segregated than red giants in all GCs, in agreement with the estimated core and half-mass relaxation times which are smaller than the cluster ages. Assuming that this is a signature of mass segregation, we conclude that AM 1 and Eridanus are slightly dynamically more evolved than Pal 3 and Pal 4.

  16. NEAR-IR PHOTOMETRIC PROPERTIES OF HB, MSTO, AND SGB FOR METAL POOR GALACTIC GLOBULAR CLUSTERS

    Directory of Open Access Journals (Sweden)

    J.-W. Kim

    2007-03-01

    Full Text Available We report photometric features of the HB, MSTO, and SGB for a set of metal-poor Galactic globular clusters on the near-IR CMDs. The magnitude and color of the MSTO and SGB are measured on the fiducial normal points of the CMDs by applying a polynomial fit. The near-IR luminosity functions of horizontal branch stars in the classical second parameter pair M3 and M13 indicate that HB stars in M13 are dominated by hot stars that are rotatively faint in the infrared, whereas HB stars in M3 are brighter than those in M13. The luminosity functions of HB stars in the observed bulge clusters, except for NGC 6717, show a trend that the fainter hot HB stars are dominated in the relatively metal-poor clusters while the relatively metal-rich clusters contain the brighter HB stars. It is suggestive that NGC 6717 would be an extreme example of the second-parameter phenomenon for the bulge globular clusters.

  17. The Hot Stellar Content and HB morphology of the massive globular cluster G1

    Science.gov (United States)

    Rich, R.

    2010-09-01

    We propose to obtain deep WFC3 imagery of the Local Group's most luminous globular cluster, G1. Our primary aim is to define the hot stellar content and the extent of what appears to be a multimodal horizontal branch, analogous to those known in Omega Cen and NGC 2808. G1 is 40 kpc distant in the M31, and it would have been highly unlikely that collision with a giant molecular clould would be responsible for the complex populations which must therefore be the result of self-enrichment. We will obtain data very similar to those obtained for the known Galactic multimodal globular clusters NGC 6388 and 6441, and compare the stellar distribution on the horizontal branch with models. We can constrain the fraction of helium-enriched stars, if present, and search for supra-horizontal branch and other anomalous hot, evolved, stars. Parallel ACS observations will be the deepest ever obtained in the adjacnt field to G1, and will help to constrain whether G1 was the nucleus of a now disrupted galaxy.

  18. Effects of main-sequence mass loss on the turnoff ages of globular clusters

    International Nuclear Information System (INIS)

    Guzik, J.A.

    1989-01-01

    Willson, Bowen, and Struck-Marcell have proposed that globular cluster main-sequence turnoff ages can be reconciled with the lower ages of the Galaxy and universe deduced from other methods by incorporating an epoch of early main-sequence mass-loss by stars of spectral types A through early-F. The proposed mass loss is pulsation-driven, and facilitated by rapid rotation. This paper presents stellar evolution calculations of Pop. II (Z = 0.001) mass-losing stars of initial mass 0.8 to 1.6 M circle dot , with exponentially-decreasing mass loss rates of e-folding times 0.5 to 2.0 Gyr, evolving to a final mass of 0.7 M circle dot . The calculations indicate that a globular cluster with apparent turnoff age 18 Gyr could have an actual age as low as ∼12 Gyr. Observational implications that may help to verify the hypothesis, e.g. low C/N abundance ratios among red giants following first dredge-up, blue stragglers, red giant deficiencies, and signatures in cluster mass/luminosity functions, are also discussed.25 refs., 4 figs., 3 tabs

  19. Chemical Abundances of Red Giant Branch Stars in the Globular Cluster NGC 288

    Science.gov (United States)

    Hsyu, Tiffany; Johnson, C. I.; Pilachowski, C. A.; Lee, Y.; Rich, R. M.

    2013-01-01

    We present chemical abundances and radial velocities for ~30 red giant branch (RGB) stars in the globular cluster NGC 288. The results are based on moderate resolution (R≈18,000) and moderate signal-to-noise ratio 50-75) obtained with the Hydra multi-object spectrograph on the Blanco 4m telescope. NGC 288 has been shown to exhibit two separate RGBs and we investigate possible differences in metallicity and/or light element abundances between stars on each branch. We present a new filter tracing for the CTIO Calcium HK narrow band filter and explore its effects on previous globular cluster color-magnitude diagrams. We also compare the light element abundance patterns of NGC 288 to those of other similar metallicity halo clusters. This material is based upon work supported by the National Science Foundation under award No.AST-1003201 to C.I.J. C.A.P. gratefully acknowledges support from the Daniel Kirkwood Research Fund at Indiana University. R.M.R. acknowledges support from NSF grants AST-0709479 and AST-121120995.

  20. Photometric analyses of abundances in dwarf spheroidal galaxies and globular clusters

    International Nuclear Information System (INIS)

    Light, R.M.

    1988-01-01

    This study investigated the abundance characteristics of several dwarf spheroidal galaxies. The chemical properties of stars in these galaxies are tracers of the origin and evolution of their stellar populations, and thus can provide constraints on the theories of their formation. To derive this abundance information, photometric observations of stars in a sample of globular clusters, covering a large range in metallicity, were analyzed. Parameters describing the position of the red giant branch were found to correlate very well with cluster metallicity over a large range in abundance. These measurements, made in the Thuan-Gunn photometry system, provide ranking schemes which are, with accurate photometry, more sensitive to changes in metallicity than similar broadband BV parameters. The relations were used to derive an improved estimate of the metallicity of cluster NGC 5053. These metallicity relations were used to analyze the Thuan-Gunn system photometry produced for the Sculptor, Ursa Minor, and Carina galaxies. The excellent agreement between their metallicities and those from other previous studies indicates that globular cluster red giant branch parameters are very useful in ranking dwarf spheroidal populations by metallicity. Together with other galaxian data, strong correlations can be seen between the mean metallicities and dispersions in metallicity and the luminosities of the dwarf spheroidal galaxies. These trends also seem to apply to members of the dwarf elliptical class of galaxies. The ramifications that these correlations and the existence of a metallicity gradient in Sculptor have on the formation of the dwarf spheroidals are discussed

  1. VizieR Online Data Catalog: Tidal radii of 7 globular clusters (Lehmann+ 1997)

    Science.gov (United States)

    Lehmann, I.; Scholz, R.-D.

    1998-02-01

    We present new tidal radii for seven Galactic globular clusters using the method of automated star counts on Schmidt plates of the Tautenburg, Palomar and UK telescopes. The plates were fully scanned with the APM system in Cambridge (UK). Special account was given to a reliable background subtraction and the correction of crowding effects in the central cluster region. For the latter we used a new kind of crowding correction based on a statistical approach to the distribution of stellar images and the luminosity function of the cluster stars in the uncrowded area. The star counts were correlated with surface brightness profiles of different authors to obtain complete projected density profiles of the globular clusters. Fitting an empirical density law (King 1962AJ.....67..471K) we derived the following structural parameters: tidal radius rt, core radius rc and concentration parameter c. In the cases of NGC 5466, M 5, M 12, M 13 and M 15 we found an indication for a tidal tail around these objects (cf. Grillmair et al., 1995AJ....109.2553G). (1 data file).

  2. Sugar and protein digestion in flowerpiercers and hummingbirds: a comparative test of adaptive convergence.

    Science.gov (United States)

    Schondube, J E; Martinez del Rio, C

    2004-04-01

    Flowerpiercers are the most specialized nectar-feeding passerines in the Neotropics. They are nectar robbers that feed on the sucrose-rich diet of hummingbirds. To test the hypothesis that flowerpiercers have converged with hummingbirds in digestive traits, we compared the activity of intestinal enzymes and the gut nominal area of cinnamon-bellied flowerpiercers (Diglossa baritula) with those of eleven hummingbird species. We measured sucrase, maltase, and aminopeptidase-N activities. To provide a comparative context, we also compared flowerpiercers and hummingbirds with 29 species of passerines. We analyzed enzyme activity using both standard allometric analyses and phylogenetically independent contrasts. Both approaches revealed the same patterns. With the exception of sucrase activity, hummingbirds' digestive traits were indistinguishable from those of passerines. Sucrase activity was ten times higher in hummingbirds than in passerines. Hummingbirds and passerines also differed in the relationship between intestinal maltase and sucrase activities. Maltase activity was two times higher per unit of sucrase activity in passerines than in hummingbirds. The sucrase activity of D. baritula was much lower than that of hummingbirds, and not unlike that expected for a passerine of its body mass. With the exception of aminopeptidase-N activity, the digestive traits of D. baritula were not different from those of other passerines. Copyright 2004 Springer-Verlag

  3. Dynamics of proteins and of their hydration layer studied by neutron scattering and additional biophysical methods

    International Nuclear Information System (INIS)

    Gallat, Francois-Xavier

    2011-01-01

    This thesis work focused on the dynamics of proteins, surrounded by their hydration layer, a water shell around the protein vital for its biological function. Each of these components is accompanied by a specific dynamics which union reforms the complex energy landscape of the system. The joint implementation of selective deuteration, incoherent neutron scattering and tera-hertz spectroscopy allowed to explore the dynamics of proteins and that of the hydration shell. The influence of the folding state of protein on its dynamics has been studied by elastic neutron scattering. Globular proteins were less dynamic than its intrinsically disordered analogues. Themselves appear to be stiffer than non-physiological unfolded proteins. The oligomerization state and the consequences on the dynamics were investigated. Aggregates of a globular protein proved to be more flexible than the soluble form. In contrast, aggregates of a disordered protein showed lower average dynamics compared to the soluble form. These observations demonstrate the wide range of dynamics among the proteome. Incoherent neutron scattering experiences on the hydration layer of globular and disordered proteins have yielded information on the nature of water motion around these proteins. The measurements revealed the presence of translational motions concomitant with the onset of the transition dynamics of hydration layers, at 220 K. Measurements have also shown a stronger coupling between a disordered protein and its hydration water, compared to a globular protein and its hydration shell. The nature of the hydration layer and its influence on its dynamics has been explored with the use of polymers that mimic the water behavior and that act as a source of flexibility for the protein. Eventually, the dynamics of methyl groups involved in the dynamical changes observed at 150 and 220 K, was investigated. (author) [fr

  4. Total protein

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/003483.htm Total protein To use the sharing features on this page, please enable JavaScript. The total protein test measures the total amount of two classes ...

  5. Motor and memory testing of long-lived pregnancy-associated plasma protein--a knock-out mice.

    Science.gov (United States)

    Mason, Emily J; Grell, Jacquelyn A; West, Sally A; Conover, Cheryl A

    2014-12-01

    Mice deficient in pregnancy-associated plasma protein-A (PAPP-A), an IGF binding protein protease, have been shown to be resistant to experimentally induced atherosclerosis and diabetic nephropathy, and, in the laboratory environment, live 30-40% longer than wild-type littermates in association with delayed incidence and occurrence of age-related neoplasms and degenerative diseases. PAPP-A is highly expressed in the cerebellum and hippocampus of the mouse brain. Therefore, the studies presented here were aimed at determining motor behavior, learning and retention in PAPP-A knock-out (KO) mice compared to wild-type (WT) littermates with age. Balance and coordination were assessed using an accelerating rotarod; learning and memory were assessed in a Stone T-maze. Time on the rotarod decreased with age but there was no significant difference between PAPP-A KO and WT mice at any of the testing ages. Latency to reach the goal box and number of errors committed in the Stone T-maze did not change with age and there were no significant differences between PAPP-A KO and WT mice. Lack of PAPP-A in mice did not impact central regulation of coordination, learning or memory. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. On the importance of the distance measures used to train and test knowledge-based potentials for proteins.

    Directory of Open Access Journals (Sweden)

    Martin Carlsen

    Full Text Available Knowledge-based potentials are energy functions derived from the analysis of databases of protein structures and sequences. They can be divided into two classes. Potentials from the first class are based on a direct conversion of the distributions of some geometric properties observed in native protein structures into energy values, while potentials from the second class are trained to mimic quantitatively the geometric differences between incorrectly folded models and native structures. In this paper, we focus on the relationship between energy and geometry when training the second class of knowledge-based potentials. We assume that the difference in energy between a decoy structure and the corresponding native structure is linearly related to the distance between the two structures. We trained two distance-based knowledge-based potentials accordingly, one based on all inter-residue distances (PPD, while the other had the set of all distances filtered to reflect consistency in an ensemble of decoys (PPE. We tested four types of metric to characterize the distance between the decoy and the native structure, two based on extrinsic geometry (RMSD and GTD-TS*, and two based on intrinsic geometry (Q* and MT. The corresponding eight potentials were tested on a large collection of decoy sets. We found that it is usually better to train a potential using an intrinsic distance measure. We also found that PPE outperforms PPD, emphasizing the benefits of capturing consistent information in an ensemble. The relevance of these results for the design of knowledge-based potentials is discussed.

  7. My 65 years in protein chemistry.

    Science.gov (United States)

    Scheraga, Harold A

    2015-05-01

    This is a tour of a physical chemist through 65 years of protein chemistry from the time when emphasis was placed on the determination of the size and shape of the protein molecule as a colloidal particle, with an early breakthrough by James Sumner, followed by Linus Pauling and Fred Sanger, that a protein was a real molecule, albeit a macromolecule. It deals with the recognition of the nature and importance of hydrogen bonds and hydrophobic interactions in determining the structure, properties, and biological function of proteins until the present acquisition of an understanding of the structure, thermodynamics, and folding pathways from a linear array of amino acids to a biological entity. Along the way, with a combination of experiment and theoretical interpretation, a mechanism was elucidated for the thrombin-induced conversion of fibrinogen to a fibrin blood clot and for the oxidative-folding pathways of ribonuclease A. Before the atomic structure of a protein molecule was determined by x-ray diffraction or nuclear magnetic resonance spectroscopy, experimental studies of the fundamental interactions underlying protein structure led to several distance constraints which motivated the theoretical approach to determine protein structure, and culminated in the Empirical Conformational Energy Program for Peptides (ECEPP), an all-atom force field, with which the structures of fibrous collagen-like proteins and the 46-residue globular staphylococcal protein A were determined. To undertake the study of larger globular proteins, a physics-based coarse-grained UNited-RESidue (UNRES) force field was developed, and applied to the protein-folding problem in terms of structure, thermodynamics, dynamics, and folding pathways. Initially, single-chain and, ultimately, multiple-chain proteins were examined, and the methodology was extended to protein-protein interactions and to nucleic acids and to protein-nucleic acid interactions. The ultimate results led to an understanding

  8. VLT/UVES spectroscopy of individual stars in three globular clusters in the Fornax dwarf spheroidal galaxy

    NARCIS (Netherlands)

    Letarte, B; Hill, [No Value; Jablonka, P; Tolstoy, E; Francois, P; Meylan, G

    We present a high resolution ( R similar to 43 000) abundance analysis of a total of nine stars in three of the five globular clusters associated with the nearby Fornax dwarf spheroidal galaxy. These three clusters ( 1, 2 and 3) trace the oldest, most metal-poor stellar populations in Fornax. We

  9. X-Ray and optical study of low core density globular clusters NGC6144 and E3

    NARCIS (Netherlands)

    Lan, S.-H.; Kong, A.K.H.; Verbunt, F.W.M.; Lewin, W.H.G.; Bassa, C.G.; Anderson, S.F.; Pooley, D.

    2010-01-01

    We report on the Chandra X-ray Observatory and Hubble Space Telescope (HST) observations of two low coredensity globular clusters, NGC6144 and E3. By comparing the number of X-ray sources inside the half-mass radius to those outside, we found six X-ray sources within the half-mass radius of NGC6144,

  10. Data Analysis of Globular Cluster Harris Catalogue in View of the King Models and Their Dynamical Evolution. I. Theoretical model

    Directory of Open Access Journals (Sweden)

    Marco Merafina

    2014-12-01

    Full Text Available We discuss the possibility to analyze the problem of gravothermal catastrophe in a new way, by obtaining thermodynamical equations to apply to a selfgravitating system. By using the King distribution function in the framework of statistical mechanics we treat the globular clusters evolution as a sequence of quasi-equilibrium thermodynamical states.

  11. The first two transient supersoft X-ray sources in M 31 globular clusters and the connection to classical novae

    Czech Academy of Sciences Publication Activity Database

    Henze, M.; Pietsch, W.; Haberl, F.; Sala, G.; Quimby, R.; Hernanz, M.; Della Valle, M.; Milne, P.; Williams, G.G.; Burwitz, V.; Greiner, J.; Stiele, H.; Hartmann, D. H.; Kong, A. K. H.; Hornoch, Kamil

    2009-01-01

    Roč. 500, č. 2 (2009), s. 769-779 ISSN 0004-6361 Institutional research plan: CEZ:AV0Z10030501 Keywords : M 31 * novae * globular clusters Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 4.179, year: 2009

  12. Discovery of a 205.89 Hz accreting millisecond X-ray pulsar in the globular cluster NGC 6440

    NARCIS (Netherlands)

    Altamirano, D.; Patruno, A.; Heinke, C.O.; Markwardt, C.; Strohmayer, T.E.; Linares, M.; Wijnands, R.; van der Klis, M.; Swank, J.H.

    2010-01-01

    We report on the discovery of the second accreting millisecond X-ray pulsar (AMXP) in the globular cluster NGC 6440. Pulsations with a frequency of 205.89 Hz were detected with RXTE on 2009 August 30, October 1 and October 28, during the decays of less than or similar to 4 day outbursts of a newly

  13. Nonlinear Color–Metallicity Relations of Globular Clusters. VII. Nonlinear Absorption-line Index versus Metallicity Relations and Bimodal Index Distributions of NGC 5128 Globular Clusters

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Sooyoung; Yoon, Suk-Jin, E-mail: sjyoon0691@yonsei.ac.kr [Department of Astronomy and Center for Galaxy Evolution Research, Yonsei University, Seoul 120-749 (Korea, Republic of)

    2017-07-01

    Spectroscopy on the globular cluster (GC) system of NGC 5128 revealed bimodality in absorption-line index distributions of its old GCs. GC division is a widely observed and studied phenomenon whose interpretation has depicted host galaxy formation and evolution such that it harbors two distinct metallicity groups. Such a conventional view of GC bimodality has mainly been based on photometry. The recent GC photometric data, however, presented an alternative perspective in which the nonlinear metallicity-to-color transformation is responsible for color bimodality of GC systems. Here we apply the same line of analysis to the spectral indices and examine the absorption-line index versus metallicity relations for the NGC 5128 GC system. NGC 5128 GCs display nonlinearity in the metallicity-index planes, most prominently for the Balmer lines and by a non-negligible degree for the metallicity-sensitive magnesium line. We demonstrate that the observed spectroscopic division of NGC 5128 GCs can be caused by the nonlinear nature of the metallicity-to-index conversions and thus one does not need to resort to two separate GC subgroups. Our analysis incorporating this nonlinearity provides a new perspective on the structure of NGC 5128's GC system, and a further piece to the global picture of the formation of GC systems and their host galaxies.

  14. Fluid-crystal coexistence for proteins and inorganic nanocolloids : Dependence on ionic strength

    NARCIS (Netherlands)

    Prinsen, P.; Odijk, T.

    2006-01-01

    We investigate theoretically the fluid-crystal coexistence of solutions of globular charged nanoparticles such as proteins and inorganic colloids. The thermodynamic properties of the fluid phase are computed via the optimized Baxter model P. Prinsen and T. Odijk [J. Chem. Phys. 121, 6525 (2004)].

  15. Sequential unfolding of the two-domain protein Pseudomonas stutzeri cytochrome c(4)

    DEFF Research Database (Denmark)

    Andersen, Niels Højmark; Jensen, Thomas Jon; Nørgaard, Allan

    2002-01-01

    F stutzeri cytochrome c. is a di-haem protein, composed of two globular domains each with His-Met coordinated haem. and a hydrogen bond network between the domains. The domain foldings are highly symmetric but with specific differences including structural differences of ligand coordination, and ...

  16. Test

    DEFF Research Database (Denmark)

    Bendixen, Carsten

    2014-01-01

    Bidrag med en kortfattet, introducerende, perspektiverende og begrebsafklarende fremstilling af begrebet test i det pædagogiske univers.......Bidrag med en kortfattet, introducerende, perspektiverende og begrebsafklarende fremstilling af begrebet test i det pædagogiske univers....

  17. THE HUBBLE SPACE TELESCOPE UV LEGACY SURVEY OF GALACTIC GLOBULAR CLUSTERS. III. A QUINTUPLE STELLAR POPULATION IN NGC 2808

    International Nuclear Information System (INIS)

    Milone, A. P.; Marino, A. F.; Jerjen, H.; Piotto, G.; Renzini, A.; Bedin, L. R.; Anderson, J.; Bellini, A.; Cassisi, S.; Pietrinferni, A.; D’Antona, F.; Ventura, P.

    2015-01-01

    In this study we present the first results from multi-wavelength Hubble Space Telescope (HST) observations of the Galactic globular cluster (GC) NGC 2808 as an extension of the Hubble Space Telescope UV Legacy Survey of Galactic GCs (GO-13297 and previous proprietary and HST archive data). Our analysis allowed us to disclose a multiple-stellar-population phenomenon in NGC 2808 even more complex than previously thought. We have separated at least five different populations along the main sequence and the red giant branch (RGB), which we name A, B, C, D, and E (though an even finer subdivision may be suggested by the data). We identified the RGB bump in four out of the five RGBs. To explore the origin of this complex color–magnitude diagram, we have combined our multi-wavelength HST photometry with synthetic spectra, generated by assuming different chemical compositions. The comparison of observed colors with synthetic spectra suggests that the five stellar populations have different contents of light elements and helium. Specifically, if we assume that NGC 2808 is homogeneous in [Fe/H] (as suggested by spectroscopy for Populations B, C, D, E, but lacking for Population A) and that population A has a primordial helium abundance, we find that populations B, C, D, E are enhanced in helium by ΔY ∼ 0.03, 0.03, 0.08, 0.13, respectively. We obtain similar results by comparing the magnitude of the RGB bumps with models. Planned spectroscopic observations will test whether Population A also has the same metallicity, or whether its photometric differences with Population B can be ascribed to small [Fe/H] and [O/H] differences rather than to helium

  18. To the Edge of M87 and Beyond: Spectroscopy of Intracluster Globular Clusters and Ultracompact Dwarfs in the Virgo Cluster

    Energy Technology Data Exchange (ETDEWEB)

    Ko, Youkyung; Lee, Myung Gyoon; Jang, In Sung [Astronomy Program, Department of Physics and Astronomy, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826 (Korea, Republic of); Hwang, Ho Seong [Quantum Universe Center, Korea Institute for Advanced Study, 85 Hoegiro, Dongdaemun-gu, Seoul 02455 (Korea, Republic of); Park, Hong Soo; Hwang, Narae; Park, Byeong-Gon [Korea Astronomy and Space Science Institute, 776 Daedeokdae-Ro, Yuseong-Gu, Daejeon 34055 (Korea, Republic of); Lim, Sungsoon [Department of Astronomy, Peking University, Beijing 100871 (China); Sohn, Jubee, E-mail: ykko@astro.snu.ac.kr, E-mail: mglee@astro.snu.ac.kr [Smithsonian Astrophysical Observatory, 60 Garden Street, Cambridge, MA 02138 (United States)

    2017-02-01

    We present the results of a wide-field spectroscopic survey of globular clusters (GCs) in the Virgo cluster. We obtain spectra for 201 GCs and 55 ultracompact dwarfs (UCDs) using Hectospec on the Multiple-Mirror Telescope and derive their radial velocities. We identify 46 genuine intracluster GCs (IGCs), not associated with any Virgo galaxies, using the 3D GMM test on the spatial and radial velocity distribution. They are located at a projected distance 200 kpc≲R ≲500 kpc from the center of M87. The radial velocity distribution of these IGCs shows two peaks, one at v{sub r} = 1023 km s{sup −1}, associated with the Virgo main body, and another at v {sub r}=36 km s{sup −1}, associated with the infalling structure. The velocity dispersion of the IGCs in the Virgo main body is σ{sub GC}∼314 km s{sup −1}, which is smoothly connected to the velocity dispersion profile of M87 GCs but is much lower than that of dwarf galaxies in the same survey field, σ {sub dwarf}∼608 km s{sup −1}. The UCDs are more centrally concentrated on massive galaxies - M87, M86, and M84. The radial velocity dispersion of the UCD system is much smaller than that of dwarf galaxies. Our results confirm the large-scale distribution of Virgo IGCs indicated by previous photometric surveys. The color distribution of the confirmed IGCs shows a bimodality similar to that of M87 GCs. This indicates that most IGCs are stripped off dwarf galaxies and some off massive galaxies in the Virgo.

  19. Protein fiber linear dichroism for structure determination and kinetics in a low-volume, low-wavelength couette flow cell

    OpenAIRE

    Dafforn, Tim; Rajendra, Jacindra; Halsall, David J.; Serpell, Louise C.; Rodger, Alison

    2004-01-01

    High-resolution structure determination of soluble globular proteins relies heavily on x-ray crystallography techniques. Such an approach is often ineffective for investigations into the structure of fibrous proteins as these proteins generally do not crystallize. Thus investigations into fibrous protein structure have relied on less direct methods such as x-ray fiber diffraction and circular dichroism. Ultraviolet linear dichroism has the potential to provide additional information on the st...

  20. Interpretation of NMR relaxation properties of Pin1, a two-domain protein, based on Brownian dynamic simulations

    International Nuclear Information System (INIS)

    Bernado, Pau; Fernandes, Miguel X.; Jacobs, Doris M.; Fiebig, Klaus; Garcia de la Torre, Jose; Pons, Miquel

    2004-01-01

    Many important proteins contain multiple domains connected by flexible linkers. Inter-domain motion is suggested to play a key role in many processes involving molecular recognition. Heteronuclear NMR relaxation is sensitive to motions in the relevant time scales and could provide valuable information on the dynamics of multi-domain proteins. However, the standard analysis based on the separation of global tumbling and fast local motions is no longer valid for multi-domain proteins undergoing internal motions involving complete domains and that take place on the same time scale than the overall motion.The complexity of the motions experienced even for the simplest two-domain proteins are difficult to capture with simple extensions of the classical Lipari-Szabo approach. Hydrodynamic effects are expected to dominate the motion of the individual globular domains, as well as that of the complete protein. Using Pin1 as a test case, we have simulated its motion at the microsecond time scale, at a reasonable computational expense, using Brownian Dynamic simulations on simplified models. The resulting trajectories provide insight on the interplay between global and inter-domain motion and can be analyzed using the recently published method of isotropic Reorientational Mode Dynamics which offer a way of calculating their contribution to heteronuclear relaxation rates. The analysis of trajectories computed with Pin1 models of different flexibility provides a general framework to understand the dynamics of multi-domain proteins and explains some of the observed features in the relaxation rate profile of free Pin1